PMID- 42959076 OWN - NLM STAT- Publisher LR - 20261030 IS - 2434-9194 (Electronic) IS - 2434-9186 (Linking) VI - 8 IP - 5 DP - 2026 Oct 31 TI - Association of NCCRP1 expression with T category and in vitro growth-related characteristics of head and neck squamous cell carcinoma FaDu cells. PG - 355-362 LID - 10.35772/ghm.2026.01070 [doi] AB - Nonspecific cytotoxic cell receptor protein 1 (NCCRP1), which is expressed in normal squamous epithelium, has been reported as a prognostic marker for esophageal squamous cell carcinoma and triple-negative breast cancer. Here, we evaluated expression of NCCRP1 in head and neck squamous cell carcinoma (HNSCC) and adjacent normal mucosa, its association with clinicopathological features, and in vitro growth-related characteristics following forced NCCRP1 expression. In the present study, we evaluated expression levels of NCCRP1 in formalin-fixed, paraffin-embedded samples of specimens collected by biopsy and resection from 43 patients with HNSCC and examined association of NCCRP1 expression with clinicopathological characteristics. Human HNSCC FaDu cells with forced NCCRP1 expression were assessed using the MTT assay and the colony formation assay. We found that NCCRP1 was expressed in noncancerous spinosum and superficial epithelial layers of the oral cavity, nasopharynx, mesopharynx, hypopharynx, and larynx. NCCRP1 expression in HNSCC tissues showed considerable variation, contrasting to relatively uniform expression in noncancerous surrounding mucosa. Based on NCCRP1 staining proportion in tumor, 31 of the 43 patients (72.1%) were classified as NCCRP1-low and the remaining 12 (27.9%) patients as NCCRP1-high. NCCRP1 expression in HNSCC tissue showed an exploratory association with T category (p = 0.0215). HNSCC FaDu cells with forced NCCRP1 expression exhibited enhanced in vitro growth-related characteristics measured by the MTT and colony formation assays. NCCRP1 expression was associated with T category in this selected HNSCC cohort, and forced NCCRP1 overexpression in FaDu cells was associated with increased in vitro growth-related measurements. Nevertheless, these exploratory findings require validation in independent clinical cohorts and experimental models. CI - 2026, National Center for Global Health and Medicine. FAU - Koda, Hanako AU - Koda H AD - Department of Surgery, National Center for Global Health and Medicine, Japan Institute for Health Security, Tokyo, Japan. AD - Clinical Research Advancement Section, National Institute of Global Health and Medicine, Japan Institute for Health Security, Tokyo, Japan. AD - Course of Advanced and Specialized Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan. FAU - Hagiwara, Teruki AU - Hagiwara T AD - Clinical Research Advancement Section, National Institute of Global Health and Medicine, Japan Institute for Health Security, Tokyo, Japan. FAU - Yamada, Kazuhiko AU - Yamada K AD - Department of Surgery, National Center for Global Health and Medicine, Japan Institute for Health Security, Tokyo, Japan. AD - Course of Advanced and Specialized Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan. FAU - Yagi, Shusuke AU - Yagi S AD - Department of Surgery, National Center for Global Health and Medicine, Japan Institute for Health Security, Tokyo, Japan. AD - Clinical Research Advancement Section, National Institute of Global Health and Medicine, Japan Institute for Health Security, Tokyo, Japan. AD - Course of Advanced and Specialized Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan. FAU - Igari, Toru AU - Igari T AD - Pathology Division of Clinical Laboratory, National Center for Global Health and Medicine, Japan Institute for Health Security, Tokyo, Japan. FAU - Kawamura, Yuki I AU - Kawamura YI AD - Clinical Research Advancement Section, National Institute of Global Health and Medicine, Japan Institute for Health Security, Tokyo, Japan. AD - Course of Advanced and Specialized Medicine, Juntendo University Graduate School of Medicine, Tokyo, Japan. LA - eng PT - Journal Article PL - Japan TA - Glob Health Med JT - Global health & medicine JID - 101771579 PMC - PMC13716684 OTO - NOTNLM OT - T category OT - growth-related characteristics OT - head and neck squamous cell carcinoma OT - nonspecific cytotoxic cell receptor protein 1 COIS- The authors have no conflicts of interest to disclose. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 05:02 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/06/10 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/30 05:02 [entrez] AID - 10.35772/ghm.2026.01070 [doi] PST - ppublish SO - Glob Health Med. 2026 Oct 31;8(5):355-362. doi: 10.35772/ghm.2026.01070. PMID- 42959073 OWN - NLM STAT- Publisher LR - 20261030 IS - 2297-055X (Print) IS - 2297-055X (Linking) VI - 13 DP - 2026 TI - Coronary artery compression in percutaneous pulmonary valve implantation: a case series. PG - 1917017 LID - 10.3389/fcvm.2026.1917017 [doi] AB - Catastrophic complications of coronary artery compression after percutaneous pulmonary valve implantation (PPVI) are uncommon in patients with a dysfunctional native right ventricular outflow tract after Tetralogy of Fallot repair (rTOF). Nonetheless, we report three rTOFs with a high risk of compromising coronary blood flow for PPVI. This risk has been elucidated by multimodal assessments, including robust pre-procedural cardiac computed tomography and meticulous balloon interrogation with selective coronary angiography. CI - (c) 2026 Buntharikpornpun, Roymanee, Promphan and Duong. FAU - Buntharikpornpun, Rujira AU - Buntharikpornpun R AD - Division of Pediatric Cardiology, Department of Pediatrics, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand. FAU - Roymanee, Supaporn AU - Roymanee S AD - Division of Pediatric Cardiology, Department of Pediatrics, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand. FAU - Promphan, Worakan AU - Promphan W AD - Pediatric Heart Center, Department of Medical Services, Ministry of Public Health, Queen Sirikit National Institute of Child Health, Bangkok, Thailand. FAU - Duong, Phuoc AU - Duong P AD - Alder Hey Children's Hospital, Liverpool, United Kingdom. LA - eng PT - Case Reports PT - Journal Article DEP - 20261015 PL - Switzerland TA - Front Cardiovasc Med JT - Frontiers in cardiovascular medicine JID - 101653388 PMC - PMC13716547 OTO - NOTNLM OT - case report OT - coronary compression OT - percutaneous OT - pulmonary valve replacement OT - transcatheter COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 05:02 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/06/23 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/30 05:02 [entrez] AID - 10.3389/fcvm.2026.1917017 [doi] PST - epublish SO - Front Cardiovasc Med. 2026 Oct 15;13:1917017. doi: 10.3389/fcvm.2026.1917017. eCollection 2026. PMID- 42959063 OWN - NLM STAT- Publisher LR - 20261030 IS - 1664-1078 (Print) IS - 1664-1078 (Linking) VI - 17 DP - 2026 TI - Association between infant crawling characteristics and fundamental movement skill development in preschool children. PG - 1944438 LID - 10.3389/fpsyg.2026.1944438 [doi] AB - This study examined the associations between infant crawling experience and crawling characteristics and fundamental movement skill (FMS) development in preschool children to characterize population-level associations and generate hypotheses for prospective research. A retrospective cross-sectional design was used, including 557 children aged 3-6 years from kindergartens in Qufu, Shandong Province, and the surrounding areas. Infant crawling experience and crawling characteristics were collected retrospectively through guardian questionnaires, and FMS was assessed using the Test of Gross Motor Development-Third Edition (TGMD-3). Children with a total FMS score below the 25th percentile were classified as having relatively insufficient FMS development, and a multivariable logistic regression analysis was performed to examine these associations. Compared with children with relatively sufficient crawling experience, those with insufficient crawling experience had lower locomotor skill and ball skill scores, as well as lower total FMS scores. These findings suggest that insufficient crawling experience and selected crawling characteristics, particularly daily crawling duration and crawling-posture diversity, are statistically associated with relatively insufficient FMS development in preschool children; however, their significance requires further confirmation in prospective studies. CI - Copyright (c) 2026 Zhou, Yin, Fang, Chen, Wu and Yuan. FAU - Zhou, Yuehui AU - Zhou Y AD - School of Sport Science, Qufu Normal University, Qufu, Shandong, China. FAU - Yin, Huizi AU - Yin H AD - School of Physical Education, Northeast Normal University, Jilin, China. FAU - Fang, Yingxiang AU - Fang Y AD - Yantai Development Zone Experimental Middle School, Shandong, China. FAU - Chen, Bingao AU - Chen B AD - School of Sports,Yunnan Normal University, Kunming, China. FAU - Wu, Juhua AU - Wu J AD - School of Sports, Guangxi University of Science and Technology, Liuzhou, Guangxi, China. FAU - Yuan, Yang AU - Yuan Y AD - School of Sport Science, Qufu Normal University, Qufu, Shandong, China. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Front Psychol JT - Frontiers in psychology JID - 101550902 PMC - PMC13716658 OTO - NOTNLM OT - crawling experience OT - fundamental movement skills OT - infant crawling OT - insufficient crawling experience OT - preschool children COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 05:00 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/07/21 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/30 05:00 [entrez] AID - 10.3389/fpsyg.2026.1944438 [doi] PST - epublish SO - Front Psychol. 2026 Oct 15;17:1944438. doi: 10.3389/fpsyg.2026.1944438. eCollection 2026. PMID- 42959051 OWN - NLM STAT- Publisher LR - 20261030 IS - 1664-2392 (Print) IS - 1664-2392 (Linking) VI - 17 DP - 2026 TI - A predictive model for osteoporosis in frail elderly patients with cerebral infarction: an integrated analysis based on machine learning and care-sensitive indicators. PG - 1771964 LID - 10.3389/fendo.2026.1771964 [doi] AB - OBJECTIVE: To identify key risk factors for osteoporosis in elderly patients with frailty and cerebral infarction using three machine learning algorithms-Least Absolute and Selective Operator (LASSO) regression, Extreme Gradient Boosting (XGBoost), and Random Forest (RF)-and establish a risk prediction model to provide data support for clinical prevention. METHODS: A retrospective study was conducted using clinical data from 120 elderly frail patients with cerebral infarction admitted to our hospital between April 2024 and October 2025. Patients were categorized into an osteoporosis occurrence group (n=41) and a non-occurrence group (n=79) based on medical records indicating osteoporosis development. Clinical data were collected and compared. Risk factors were screened using "overlap-covering" screening with three machine learning methods: LASSO regression, XGBoost, and RF. The intersecting variables were incorporated into a logistic regression model to analyze risk factors for osteoporosis in elderly frail patients with cerebral infarction. Data analysis was performed using R software. RF to screen risk factors using "overlap coverage." Intersecting variables were incorporated into logistic regression to analyze factors influencing osteoporosis in elderly patients with frailty and cerebral infarction. A nomogram prediction model was developed using R software, internally validated via Bootstrap resampling (1000 iterations), and its predictive accuracy assessed through calibration curves. Clinical utility was evaluated using decision curves. RESULTS: Significant differences (P < 0.05) were observed between the occurrence and non-occurrence groups in gender, age, affected circulatory system, disease severity, Tilburg Frailty Inventory (TFI) score, Global Nutritional Risk Index (GNRI), Barthel Index, and Self-Rating Anxiety Scale (SAS) score. Through machine learning screening using LASSO regression, XGBoost, and RF, five common risk factors for osteoporosis in elderly frail patients with cerebral infarction were identified: age, TFI score, GNRI, Barthel Index, and SAS score as five common risk factors for osteoporosis in elderly frail patients with cerebral infarction. These were incorporated into a logistic regression model. Analysis revealed that age, TFI score, and SAS score were risk factors for osteoporosis in this population (OR = 1.102, 2.876, 1.177, P<0.05). GNRI and Barthel Index were protective factors (OR = 0.913, 0.838, P<0.05). The Hosmer-Lemeshow goodness-of-fit test yielded chi(2)=2.345, P = 0.969>0.05, indicating acceptable model fit. Based on the logistic regression results, a risk prediction model for osteoporosis in elderly frail patients with cerebral infarction was constructed using a nomogram. The AUC was 0.955 (95% CI: 0.920-0.991). Calibration curves showed Cox-Snell R(2) = 0.755 and Nagelkerke R(2) = 0.546, indicating strong explanatory power and high calibration of the model. The clinical decision curve generally exceeded both extreme curves, demonstrating that the nomogram effectively captured the risk distribution. Nagelkerke R(2): 0.546, indicating strong explanatory power and high calibration for the dependent variable. The clinical decision curve generally exceeded both extreme curves, demonstrating that the factors included in the nomogram provide high net benefit for predicting osteoporosis in patients. CONCLUSION: Key factors influencing osteoporosis development in frail elderly patients with cerebral infarction include age, TFI score, GNRI, Barthel Index, and SAS score. The risk prediction model constructed using these factors demonstrates predictive value, high calibration accuracy, and clinical utility, providing theoretical guidance for clinical practice. CI - Copyright (c) 2026 Liu, Wen, Chen, Li and Xie. FAU - Liu, Yi AU - Liu Y AD - Department of Orthopedics, Zhenjiang Hospital Affiliated to Nanjing University of Chinese Medicine, Zhenjiang Hospital of Traditional Chinese Medicine, Zhenjiang, Jiangsu, China. FAU - Wen, Jing AU - Wen J AD - Department of Geriatrics, Zhenjiang Hospital Affiliated to Nanjing University of Chinese Medicine, Zhenjiang Hospital of Traditional Chinese Medicine, Zhenjiang, Jiangsu, China. FAU - Chen, Yanxia AU - Chen Y AD - Department of Orthopedics, Zhenjiang Hospital Affiliated to Nanjing University of Chinese Medicine, Zhenjiang Hospital of Traditional Chinese Medicine, Zhenjiang, Jiangsu, China. FAU - Li, Hui AU - Li H AD - Department of Nursing, Zhenjiang Hospital Affiliated to Nanjing University of Chinese Medicine, Zhenjiang Hospital of Traditional Chinese Medicine, Zhenjiang, Jiangsu, China. FAU - Xie, Panpan AU - Xie P AD - Department of Nephrology, Zhenjiang Hospital Affiliated to Nanjing University of Chinese Medicine, Zhenjiang Hospital of Traditional Chinese Medicine, Zhenjiang, Jiangsu, China. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Front Endocrinol (Lausanne) JT - Frontiers in endocrinology JID - 101555782 SB - IM PMC - PMC13716607 OTO - NOTNLM OT - cerebral infarction OT - elderly OT - frailty OT - osteoporosis OT - prediction model COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 04:59 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2025/12/20 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/30 04:59 [entrez] AID - 10.3389/fendo.2026.1771964 [doi] PST - epublish SO - Front Endocrinol (Lausanne). 2026 Oct 15;17:1771964. doi: 10.3389/fendo.2026.1771964. eCollection 2026. PMID- 42959042 OWN - NLM STAT- Publisher LR - 20261030 IS - 2297-7775 (Electronic) IS - 2297-7775 (Linking) VI - 11 DP - 2026 TI - Interdependent urban economies: a network analysis of socio-economic relationships between street vendors and formal businesses in Ratnapura, Sri Lanka. PG - 1882601 LID - 10.3389/fsoc.2026.1882601 [doi] AB - INTRODUCTION: The struggle between street vendors and authorities over access to public spaces in urban areas has drawn the attention of scholars for a long time. In the case study of Ratnapura in Sri Lanka, we aim to make visible the social network infrastructure of street vendors with a focus on two major objectives: (1) Exploration of the nature of the relationship between street vendors and formal urban space, and (2) Evaluation of the importance of street vendors for the functions of formal urban spaces. METHODS: The primary data collection methods were systematic observations and semi-structured interviews with 30 participants selected through snowball sampling. The collected data were used to develop activity maps by adapting Hakansson's Economic Network Model (ENM) and Social Network Analysis (SNA). RESULTS: The results revealed four types of relationships among the street vendors and formal shop owners-direct, indirect, uni-directional, and bi-directional-based on the three dimensions of actor bonds, activity links, and resource ties. The findings further demonstrate that street vendors interact directly with a wide range of actors and serve as bridges or intermediaries within the urban economic network. Moreover, the results indicate that street vendors do not merely survive in public spaces; they also generate indirect benefits, provide essential services, and support the functioning of other actors within the formal urban system. DISCUSSION: These findings contribute novel insights to social network analysis research by demonstrating the patterns of socio-economic relationships between street vendors and formal urban actors. Moreover, this research encourages urban planners and policymakers to recognize the contribution of street vendors to urban economic development. CI - Copyright (c) 2026 Kularathne, Meetiyagoda, Sooriyagoda and Akalanka. FAU - Kularathne, Oshini AU - Kularathne O AD - Department of Town and Country Planning, Faculty of Architecture, University of Moratuwa, Moratuwa, Sri Lanka. FAU - Meetiyagoda, Lakshika AU - Meetiyagoda L AD - Department of Town and Country Planning, Faculty of Architecture, University of Moratuwa, Moratuwa, Sri Lanka. FAU - Sooriyagoda, Kokila AU - Sooriyagoda K AD - Department of Town and Country Planning, Faculty of Architecture, University of Moratuwa, Moratuwa, Sri Lanka. FAU - Akalanka, Nimesh AU - Akalanka N AD - Institute for Geoinformatics, University of Munster, Munster, Germany. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Front Sociol JT - Frontiers in sociology JID - 101777459 PMC - PMC13716598 OTO - NOTNLM OT - economic network OT - inequality OT - public space OT - social network OT - street vendors OT - urban economy COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 04:58 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/15 00:00 [received] PHST- 2026/09/07 00:00 [revised] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/30 04:58 [entrez] AID - 10.3389/fsoc.2026.1882601 [doi] PST - epublish SO - Front Sociol. 2026 Oct 15;11:1882601. doi: 10.3389/fsoc.2026.1882601. eCollection 2026. PMID- 42959034 OWN - NLM STAT- Publisher LR - 20261030 IS - 2234-943X (Print) IS - 2234-943X (Linking) VI - 16 DP - 2026 TI - Case Report: Improvement in neurologic symptoms and remission following non-operative management of locally advanced cutaneous squamous cell carcinoma of the head and neck following cemiplimab therapy: two cases. PG - 1975694 LID - 10.3389/fonc.2026.1975694 [doi] AB - BACKGROUND: Cutaneous squamous cell carcinoma is a common skin malignancy in aging populations. Surgical resection and radiotherapy can result in significant morbidity, as well as functional and cosmetic impairments. PD-1 antibodies are promising treatments for locally advanced disease. CASE PRESENTATION: Two elderly men (aged 71 and 73) with locoregionally advanced head and neck tumors received neoadjuvant treatment with cemiplimab (350 mg IV every 3 weeks). Both patients presented with neurologic symptoms concerning for nerve involvement; however, perineural spread could not be identified radiographically or by limited biopsies. Both patients achieved a significant clinical response after only four doses of cemiplimab. After 4 additional doses, both patients underwent elective treatment discontinuation and surgery and radiotherapy were omitted. With over 12-month follow-up, both patients remained free of recurrence. Treatment was well-tolerated with only Grade 1 pruritus. CONCLUSION: These observations are promising and suggest the feasibility of response-adapted management following cemiplimab therapy in selected patients with advanced squamous cell skin cancer with resolution of neurologic symptoms. This approach may allow for omission of surgery and radiotherapy in selected patients. Further investigation to define appropriate candidates for this approach appears warranted. CI - Copyright (c) 2026 Lee, Samlowski, Reynolds and Sadrolashrafi. FAU - Lee, Elijah AU - Lee E AD - Kirk Kerkorian School of Medicine at University of Nevada (Las Vegas UNLV), Las Vegas, NV, United States. FAU - Samlowski, Wolfram AU - Samlowski W AD - Kirk Kerkorian School of Medicine at University of Nevada (Las Vegas UNLV), Las Vegas, NV, United States. AD - Nevada Oncology Specialists, Las Vegas, NV, United States. FAU - Reynolds, Brandon AU - Reynolds B AD - Reynolds Plastic Surgery, Las Vegas, NV, United States. FAU - Sadrolashrafi, Kaviyon AU - Sadrolashrafi K AD - Division of Dermatology, Department of Medicine, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA, United States. LA - eng PT - Case Reports PT - Journal Article DEP - 20261015 PL - Switzerland TA - Front Oncol JT - Frontiers in oncology JID - 101568867 PMC - PMC13716587 OTO - NOTNLM OT - checkpoint inhibitor OT - keratinocyte carcinoma OT - neoadjuvant therapy OT - perineural extension OT - skin cancer COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 04:57 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/08/22 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/30 04:57 [entrez] AID - 10.3389/fonc.2026.1975694 [doi] PST - epublish SO - Front Oncol. 2026 Oct 15;16:1975694. doi: 10.3389/fonc.2026.1975694. eCollection 2026. PMID- 42959028 OWN - NLM STAT- Publisher LR - 20261030 IS - 2296-858X (Print) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Ultrasound cycloplasty: dynamic efficacy and long-term safety in glaucoma patients from northwest China. PG - 1819637 LID - 10.3389/fmed.2026.1819637 [doi] AB - PURPOSE: To comprehensively evaluate the dynamic efficacy of UCP in treating different types of glaucoma patients in Northwest China, systematically analyze treatment outcome variations across follow-up time points, and assess long-term safety profiles. METHODS: In a 1-year follow-up study, 24 patients (30 eyes) with various types of glaucoma were enrolled and underwent UCP treatment (10 sectors or 8 sectors). Follow-up visits were conducted at different postoperative time points (1 day, 1 week, 1 month, 3 months, 6 months, and 1 year). Follow-up assessments consisted of IOP measurements, IOP reduction from baseline, surgical success rate, 24-hour IOP fluctuation, pain score improvement, and complications. Furthermore, the correlation between IOP at the final follow-up and baseline IOP was evaluated. RESULTS: 1. The overall IOP in all patients significantly decreased after UCP compared to preoperative levels (P < 0.01). 2. UCP demonstrated significant long-term IOP-lowering effects in patients with various types of glaucoma, with a 59.91% reduction at 1 year postoperatively. The efficacy was particularly notable in patients with primary angle-closure glaucoma, achieving a surgical success rate of 96.67% at 1 year. 3. Preoperative baseline IOP showed a positive correlation with IOP reduction at 1 year postoperatively (r (s) = 0.641, P < 0.01), indicating that higher baseline IOP was associated with greater IOP reduction. 4. UCP significantly improved IOP fluctuation, with the mean 24-hour IOP fluctuation decreasing from 10.6 preoperatively to 5.3 postoperatively (P = 0.001). 5. UCP exhibited acceptable safety, with significant reductions in pain scores (P < 0.05) and no severe complications such as hypotony or macular edema observed during follow-up. CONCLUSION: 1. In glaucoma patients in the Northwest population, UCP demonstrates significant long-term and dynamic efficacy-the higher the baseline IOP, the more pronounced the IOP-lowering effect, with outstanding performance in stabilizing IOP fluctuations. 2. UCP exhibits acceptable safety and controllable complications. Postoperative IOP stabilizes by 3 months, allowing for gradual withdrawal of medications, making it a reliable treatment option for glaucoma. CI - (c) 2026 Ge, Shi, Mu, Zhang, Yang and He. FAU - Ge, Ping AU - Ge P AD - Department of Ophthalmology, The Second Affiliated Hospital of Xi'an Medical University, Xi'an, China. AD - Department of Ophthalmology, The 986th Hospital of the Air Force, Xi'an, China. FAU - Shi, Rui AU - Shi R AD - Department of Ophthalmology, Shaanxi Provincial People's Hospital, Xi'an, China. FAU - Mu, Qing-Qing AU - Mu QQ AD - Department of Ophthalmology, The Second Affiliated Hospital of Xi'an Medical University, Xi'an, China. FAU - Zhang, Rui-Xue AU - Zhang RX AD - Department of Ophthalmology, The Second Affiliated Hospital of Xi'an Medical University, Xi'an, China. FAU - Yang, Xiang-Xiang AU - Yang XX AD - Department of Ophthalmology, The Second Affiliated Hospital of Xi'an Medical University, Xi'an, China. FAU - He, Yuan AU - He Y AD - Department of Ophthalmology, The Second Affiliated Hospital of Xi'an Medical University, Xi'an, China. LA - eng PT - Clinical Trial PT - Journal Article DEP - 20261015 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13716567 OTO - NOTNLM OT - Northwest China OT - UCP OT - dynamic efficacy OT - glaucoma OT - minimally invasive glaucoma surgery COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 04:56 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/02/28 00:00 [received] PHST- 2026/05/20 00:00 [revised] PHST- 2026/09/14 00:00 [accepted] PHST- 2026/10/30 04:56 [entrez] AID - 10.3389/fmed.2026.1819637 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 15;13:1819637. doi: 10.3389/fmed.2026.1819637. eCollection 2026. PMID- 42959019 OWN - NLM STAT- Publisher LR - 20261030 IS - 1664-2392 (Print) IS - 1664-2392 (Linking) VI - 17 DP - 2026 TI - Novel ABCC2 mutations in a neonate with Dubin-Johnson syndrome: a case report. PG - 1913891 LID - 10.3389/fendo.2026.1913891 [doi] AB - BACKGROUND: Dubin-Johnson syndrome (DJS) is a rare autosomal recessive disorder caused by mutations in the ABCC2 gene, leading to impaired excretion of conjugated bilirubin. Neonatal or infantile onset is uncommon and easily misdiagnosed as biliary atresia or progressive familial intrahepatic cholestasis. CASE PRESENTATION: We report a female infant who presented with persistent jaundice since birth (onset in the neonatal period, T (p.R815X, nonsense) inherited from the mother and c.4465_4473delinsGGCCCACAG (p.1489_1491delinsGPQ, in-frame) inherited from the father. Both mutations were classified as pathogenic/likely pathogenic according to ACMG guidelines (c.2443C>T: PVS1, PM2, PM3; c.4465_4473delinsGGCCCACAG: PM2, PM3, PM4) and have not been previously reported. Ursodeoxycholic acid therapy was administered, and bilirubin levels showed progressive decline over time; however, this improvement may also reflect the spontaneous trend toward resolution that is characteristic of the natural history of DJS. CONCLUSION: This case expands the mutational spectrum of ABCC2 gene and highlights the critical role of genetic testing in early diagnosis of infantile DJS, thereby avoiding unnecessary invasive procedures. The favorable prognosis with conservative management further supports the benign nature of this condition. CI - Copyright (c) 2026 An, Zhang and Zhang. FAU - An, Lijun AU - An L AD - Department of Nutrition, Baoding Hospital, Beijing Children's Hospital Affiliated to Capital Medical University, Baoding, Hebei, China. FAU - Zhang, Yuanda AU - Zhang Y AD - Department of Nutrition, Baoding Hospital, Beijing Children's Hospital Affiliated to Capital Medical University, Baoding, Hebei, China. FAU - Zhang, Sisi AU - Zhang S AD - Department of Gastroenterology, Baoding Hospital, Beijing Children's Hospital Affiliated to Capital Medical University, Baoding, Hebei, China. LA - eng PT - Case Reports PT - Journal Article DEP - 20261015 PL - Switzerland TA - Front Endocrinol (Lausanne) JT - Frontiers in endocrinology JID - 101555782 SB - IM PMC - PMC13716550 OTO - NOTNLM OT - ABCC2 gene OT - Dubin-Johnson syndrome OT - neonatal-onset cholestasis OT - next-generation sequencing OT - novel mutation COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 04:54 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/06/19 00:00 [received] PHST- 2026/08/20 00:00 [revised] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/30 04:54 [entrez] AID - 10.3389/fendo.2026.1913891 [doi] PST - epublish SO - Front Endocrinol (Lausanne). 2026 Oct 15;17:1913891. doi: 10.3389/fendo.2026.1913891. eCollection 2026. PMID- 42959018 OWN - NLM STAT- Publisher LR - 20261030 IS - 2234-943X (Print) IS - 2234-943X (Linking) VI - 16 DP - 2026 TI - LGALS3BP is associated with selective cargo enrichment in neuroblastoma extracellular vesicles. PG - 1966526 LID - 10.3389/fonc.2026.1966526 [doi] AB - AIM: LGALS3BP (also known as Galectin-3-binding protein, Gal-3BP, or 90 K) is a multifunctional secreted glycoprotein involved in tumor progression and has emerged as a potential biomarker and therapeutic target in several malignancies, including neuroblastoma (NB). Increasing evidence suggests that LGALS3BP contributes to shaping the NB tumor microenvironment (TME), particularly through its enrichment on tumor-derived extracellular vesicles (EVs), where it may regulate tumor-stroma interplay. We investigated whether LGALS3BP contributes to stromal cell modulation in NB and is associated with changes in EV cargo composition. METHODS: A patient-derived NB cell line naturally lacking LGALS3BP (hNB CTRL) and a matched counterpart ectopically re-expressing LGALS3BP (hNB LGALS3BP) were used. Bone marrow-derived mesenchymal stem cells (BMMSCs) were exposed to NB-derived conditioned media (CM) to assess cytokine secretion and LGALS3BP detection in recipient cells. EV cargo was analyzed by microRNA (miRNA) and proteomic profiling. Selected candidates were validated by qPCR and immunoblotting on NB-derived EVs. RESULTS: LGALS3BP-containing CM was associated with an altered BMMSCs secretory profile, while tumor-derived extracellular LGALS3BP was detected in BMMSCs. Seven miRNAs were enriched in LGALS3BP-EVs and reduced in donor cells; four were increased in BMMSCs following EV exposure. Proteomic analysis revealed enrichment of RNA-binding proteins, including SRSF1, in LGALS3BP-EVs. CONCLUSIONS: LGALS3BP is associated with altered EV cargo composition and BMMSCs secretory responses, potentially contributing to tumor-stroma communication within the NB TME. CI - Copyright (c) 2026 Cela, Konstantinidou, Amodei, Mercatelli, Piermatteo, Santoro, Pece, Staropoli, Lamolinara, Capone, Gatta and Sala. FAU - Cela, Ilaria AU - Cela I AD - Department of Innovative Technologies in Medicine and Dentistry, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. AD - Center for Advanced Studies and Technology (CAST), "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. FAU - Konstantinidou, Fani AU - Konstantinidou F AD - Department of Neuroscience, Imaging and Clinical Sciences, School of Medicine and Health Sciences, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. AD - Unit of Molecular Genetics, Center for Advanced Studies and Technology (CAST), "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. FAU - Amodei, Laura AU - Amodei L AD - Department of Innovative Technologies in Medicine and Dentistry, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. AD - Center for Advanced Studies and Technology (CAST), "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. FAU - Mercatelli, Arianna AU - Mercatelli A AD - Department of Innovative Technologies in Medicine and Dentistry, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. AD - Center for Advanced Studies and Technology (CAST), "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. FAU - Piermatteo, Francesca AU - Piermatteo F AD - Department of Innovative Technologies in Medicine and Dentistry, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. AD - Center for Advanced Studies and Technology (CAST), "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. FAU - Santoro, Sara AU - Santoro S AD - Department of Innovative Technologies in Medicine and Dentistry, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. AD - Center for Advanced Studies and Technology (CAST), "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. FAU - Pece, Asia AU - Pece A AD - Department of Innovative Technologies in Medicine and Dentistry, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. AD - Center for Advanced Studies and Technology (CAST), "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. FAU - Staropoli, Maria Clara AU - Staropoli MC AD - Department of Innovative Technologies in Medicine and Dentistry, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. AD - Center for Advanced Studies and Technology (CAST), "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. FAU - Lamolinara, Alessia AU - Lamolinara A AD - Department of Innovative Technologies in Medicine and Dentistry, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. AD - Department of Neuroscience, Imaging and Clinical Sciences, School of Medicine and Health Sciences, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. FAU - Capone, Emily AU - Capone E AD - Center for Advanced Studies and Technology (CAST), "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. AD - Department of Science, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. FAU - Gatta, Valentina AU - Gatta V AD - Department of Neuroscience, Imaging and Clinical Sciences, School of Medicine and Health Sciences, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. AD - Unit of Molecular Genetics, Center for Advanced Studies and Technology (CAST), "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. FAU - Sala, Gianluca AU - Sala G AD - Department of Innovative Technologies in Medicine and Dentistry, "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. AD - Center for Advanced Studies and Technology (CAST), "G. d'Annunzio" University of Chieti-Pescara, Chieti, Italy. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Front Oncol JT - Frontiers in oncology JID - 101568867 PMC - PMC13716570 OTO - NOTNLM OT - LGALS3BP OT - SRSF1 OT - extracellular vesicles OT - miRNA cargo enrichment OT - neuroblastoma tumor microenvironment COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 04:54 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/08/13 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/30 04:54 [entrez] AID - 10.3389/fonc.2026.1966526 [doi] PST - epublish SO - Front Oncol. 2026 Oct 15;16:1966526. doi: 10.3389/fonc.2026.1966526. eCollection 2026. PMID- 42959017 OWN - NLM STAT- Publisher LR - 20261030 IS - 2398-502X (Print) IS - 2398-502X (Linking) VI - 11 DP - 2026 TI - Incidence and Outcomes of Acute Kidney Injury in Critically Ill Patients: A Retrospective Study at a Tertiary Care Hospital. PG - 161 LID - 10.12688/wellcomeopenres.25698.2 [doi] AB - BACKGROUND: Acute kidney injury is common among critically ill patients and is associated with longer ICU stay, higher resource use and increased mortality. Evidence from low- and middle-income countries, including Nepal, remains limited. This study describes the incidence, severity distribution and clinical outcomes of AKI in a tertiary care ICU. METHODS: We conducted a hospital based retrospective observational study at Dhulikhel Hospital, Nepal. All adults admitted to the ICU from 1 (st) may 2023 to 30 (th) April 2024 were screened using ICU registry. AKI was defined and staged using KDIGO serum criteria. Demographic data, comorbidities, ICU interventions and outcomes were extracted. Associations were examined using appropriate statistical tests. RESULTS: Among 1113 patients, 1064 were enrolled for analysis. The incidence of AKI was 29.6%. Patients with AKI were older (median age 60 vs 55 years, p < 0.001), had longer ICU stays (median 3 vs 2 days, p < 0.001), were more frequently on invasive mechanical ventilation (39.6%vs 26%, p < 0.001). Type 2 diabetes mellitus was significantly associated with AKI (39.0% vs 28.0%, p = 0.001). AKI severity correlated with worse outcomes including higher mortality and adverse outcomes increasing from 12.7% in patients without AKI to 38% in stage 3 AKI. Renal replacement therapy was required in 2.3% of patients, mainly in stage 2 and 3 AKI. CONCLUSION: Acute kidney injury is a common and a serious complication among critically ill patients, associated with increased mortality and prolonged ICU stay. Early recognition and targeted management strategies, particularly for high-risk groups, are needed in resource limited settings. CI - Copyright: (c) 2026 Kharbuja k et al. FAU - Kharbuja, Kalpana AU - Kharbuja K AUID- ORCID: 0000-0002-6127-5556 AD - Department of Anaesthesia & critical care, Dhulikhel Hospital, Dhulikhel, Central Development Region, 45210, Nepal. AD - Nepal Intensive Care Research Foundation, Kathmandu, Central Development Region, Nepal. FAU - Ranjit, Sangina AU - Ranjit S AD - Department of Anaesthesia & critical care, Dhulikhel Hospital, Dhulikhel, Central Development Region, 45210, Nepal. AD - Nepal Intensive Care Research Foundation, Kathmandu, Central Development Region, Nepal. FAU - Aryal, Diptesh AU - Aryal D AD - Nepal Intensive Care Research Foundation, Kathmandu, Central Development Region, Nepal. AD - Faculty of Tropical Medicine, Mahidol University,, Mahidol Oxford Tropical Medicine Research Unit, Bangkok, Bangkok, Thailand. AD - D'Or Institute for Research and Education, Rio de Janeiro, State of Rio de Janeiro, Brazil. FAU - Luitel, Subekshya AU - Luitel S AUID- ORCID: 0009-0005-0423-8926 AD - Nepal Intensive Care Research Foundation, Kathmandu, Central Development Region, Nepal. FAU - Pandey, Ajit AU - Pandey A AUID- ORCID: 0009-0002-1667-7908 AD - Department of Anaesthesia & critical care, Dhulikhel Hospital, Dhulikhel, Central Development Region, 45210, Nepal. FAU - Timilsina, Pratikshya AU - Timilsina P AD - Department of Anaesthesia & critical care, Dhulikhel Hospital, Dhulikhel, Central Development Region, 45210, Nepal. FAU - Bajracharya, Alisha AU - Bajracharya A AD - Department of Anaesthesia & critical care, Dhulikhel Hospital, Dhulikhel, Central Development Region, 45210, Nepal. AD - Nepal Intensive Care Research Foundation, Kathmandu, Central Development Region, Nepal. FAU - Pasachhen, Sujata AU - Pasachhen S AD - Department of Anaesthesia & critical care, Dhulikhel Hospital, Dhulikhel, Central Development Region, 45210, Nepal. FAU - Bhusal, Surendra AU - Bhusal S AD - Nepal Intensive Care Research Foundation, Kathmandu, Central Development Region, Nepal. AD - Department of Anaesthesia and Critical Care, Bir Hospital, Kathmandu, Central Development Region, Nepal. LA - eng SI - figshare/10.6084/m9.figshare.30962276.V1 PT - Journal Article DEP - 20261013 PL - England TA - Wellcome Open Res JT - Wellcome open research JID - 101696457 PMC - PMC13714418 OTO - NOTNLM OT - Acute Kidney Injury (AKI) OT - critically ill patient OT - morbidity OT - outcomes COIS- No competing interests were disclosed. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 04:54 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/30 04:54 [entrez] AID - 10.12688/wellcomeopenres.25698.2 [doi] PST - epublish SO - Wellcome Open Res. 2026 Oct 13;11:161. doi: 10.12688/wellcomeopenres.25698.2. eCollection 2026. PMID- 42959016 OWN - NLM STAT- Publisher LR - 20261030 IS - 2673-253X (Electronic) IS - 2673-253X (Linking) VI - 8 DP - 2026 TI - Integration of IoT-based healthcare devices within the smart grid architecture model: a conceptual framework with economic and energy assessment for elderly care in Europe. PG - 1897840 LID - 10.3389/fdgth.2026.1897840 [doi] AB - INTRODUCTION: This study investigates the integration of IoT-enabled medical devices within the Smart Grid Architecture Model (SGAM) to support elderly home-based care in Europe. The study develops a conceptual SGAM-IoT framework that maps healthcare devices, actors, communication pathways, and functions across the SGAM interoperability layers, domains, and zones, with particular attention to interoperability, data exchange and integration between the home environment, healthcare services, and energy-aware infrastructure. METHODS: The conceptual framework is complemented by a quantitative analytical assessment of the economic and energy implications of five commonly used home-monitoring devices: a pulse oximeter, blood pressure monitor, digital thermometer, smart ECG monitor, and blood glucose monitor. The assessment relies on secondary European healthcare data, literature, manufacturer information, and market-based estimates rather than primary empirical measurements or experimental deployment. Economic feasibility is examined through Benefit-Cost Ratio (BCR), Net Present Value (NPV), and a three-scenario sensitivity analysis, while electricity consumption is estimated from rated device power and assumed operating time. RESULTS: Under the reference-scenario assumptions, the analysis produces a BCR of 2.75 and a positive NPV, while the sensitivity analysis indicates that the economic result remains positive under conservative assumptions. The revised energy assessment estimates a combined electricity consumption of approximately 0.12 kWh per month, confirming that the direct energy demand of the selected devices is marginal. DISCUSSION: These findings suggest that the main value of SGAM in this context lies not in device-level energy savings, but in providing a structured framework for interoperability, communication, data exchange, and integration with wider home-healthcare infrastructure. Potential benefits for continuity of care, autonomy and elderly well-being are discussed as expected implications requiring future empirical validation. CI - (c) 2026 Aghajani, Liscio, Scalise, Sospiro. FAU - Aghajani, Azad AU - Aghajani A AD - EUAbout, Bruxelles, Belgium. AD - Marche Polytechnic University, DIISM, Ancona, Italy. FAU - Liscio, Marco Ciro AU - Liscio MC AD - EUAbout, Bruxelles, Belgium. AD - Marche Polytechnic University, DII, Ancona, Italy. AD - Universita Telematica E Campus, DISTA, Novedrate, Italy. FAU - Scalise, Lorenzo AU - Scalise L AD - Marche Polytechnic University, DIISM, Ancona, Italy. FAU - Sospiro, Paolo AU - Sospiro P AD - EUAbout, Bruxelles, Belgium. AD - Marche Polytechnic University, DII, Ancona, Italy. AD - Universita Telematica E Campus, DISTA, Novedrate, Italy. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Front Digit Health JT - Frontiers in digital health JID - 101771889 PMC - PMC13716548 OTO - NOTNLM OT - Smart Grid Architecture Model (SGAM) OT - cost-benefit analysis (CBA) OT - digital medical devices OT - elderly healthcare OT - internet of things (IoT) COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 04:53 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/06/02 00:00 [received] PHST- 2026/08/18 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/30 04:53 [entrez] AID - 10.3389/fdgth.2026.1897840 [doi] PST - epublish SO - Front Digit Health. 2026 Oct 15;8:1897840. doi: 10.3389/fdgth.2026.1897840. eCollection 2026. PMID- 42959013 OWN - NLM STAT- Publisher LR - 20261030 IS - 1664-1078 (Print) IS - 1664-1078 (Linking) VI - 17 DP - 2026 TI - Long-term sport participation is associated with a selective auditory-musical performance profile. PG - 1758520 LID - 10.3389/fpsyg.2026.1758520 [doi] AB - Long-term sport participation has been associated with selective differences in temporal and perceptual-cognitive processing, but it remains unclear whether such differences extend to auditory-musical perception. We examined overall and component-level performance on the Montreal Battery of Evaluation of Amusia (MBEA) in 83 participants: 31 sport-trained participants and 52 psychology-student controls. Between-group differences were tested using ordinary least-squares regression with HC3 robust standard errors, adjusting for age and years of formal musical education; Holm correction was applied across the six MBEA subtests. Sport-trained participants obtained higher Total MBEA scores than controls (B = 15.34, p = 0.002). At the component level, they scored higher on the a priori temporal outcomes Rhythm (B = 2.87, Holm-adjusted p = 0.030) and Meter (B = 3.72, Holm-adjusted p = 0.012). An exploratory difference was also observed for Memory (B = 4.20, Holm-adjusted p = 0.002), whereas Contour did not remain significant after correction and no significant differences were found for Scale or Interval. Supplementary d' models and their leave-one-out sensitivity analyses converged on the same pattern for Rhythm, Meter, and Memory. Within the sport-trained group, years of sport participation were not positively associated with Total MBEA performance after adjustment for age (B = -0.78, p = 0.108). These findings suggest that long-term sport participation is associated with a selective rather than generalized profile of auditory-musical differences, with the clearest group differences in temporal processing and an exploratory difference in recognition memory. The cross-sectional design does not permit causal attribution to sport training, and the observed profile may reflect training-related adaptations, pre-existing individual differences, or both. CI - Copyright (c) 2026 Swebodzinski, Korta and Lantowski. FAU - Swebodzinski, Bartlomiej Jacek AU - Swebodzinski BJ AD - Institute of Psychology, Cardinal Stefan Wyszynski University in Warsaw, Warsaw, Poland. FAU - Korta, Kuba AU - Korta K AD - Institute of Psychology, Cardinal Stefan Wyszynski University in Warsaw, Warsaw, Poland. FAU - Lantowski, Jacek AU - Lantowski J AD - Institute of Psychology, Cardinal Stefan Wyszynski University in Warsaw, Warsaw, Poland. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Front Psychol JT - Frontiers in psychology JID - 101550902 PMC - PMC13716566 OTO - NOTNLM OT - Montreal Battery of Evaluation of Amusia OT - long-term sport participation OT - musical perception OT - perceptual-cognitive expertise OT - recognition memory OT - rhythm perception OT - temporal processing COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 04:53 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2025/12/01 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/30 04:53 [entrez] AID - 10.3389/fpsyg.2026.1758520 [doi] PST - epublish SO - Front Psychol. 2026 Oct 15;17:1758520. doi: 10.3389/fpsyg.2026.1758520. eCollection 2026. PMID- 42958975 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1660-9379 (Print) IS - 1660-9379 (Linking) VI - 22 IP - 981 DP - 2026 Oct 28 TI - [Subcutaneous administration in geriatrics : current evidence and recommendations]. PG - 1749-1753 LID - 10.53738/REVMED.2026.22.981.49160 [doi] AB - Several medications used in geriatrics are approved exclusively for intravenous administration. However, some of these agents may also be administered subcutaneously in specific clinical situations, particularly in patients with difficult venous access or when maintaining intravenous access is inappropriate. Frequently used in geriatrics and palliative care, the subcutaneous route represents a simple, minimally invasive, and generally well-tolerated alternative. In the absence of formal marketing authorization for this route of administration, its use remains the responsibility of the prescribing physician, who should rely on the available scientific evidence, assess the benefit-risk ratio for each patient, and ensure appropriate clinical monitoring. FAU - Perriard, Claire AU - Perriard C AD - Service de geriatrie, Clinique de St-Ame, Centre hospitalier du Valais romand, 1890 St-Maurice. FAU - Ancay, Xavier AU - Ancay X AD - Service de geriatrie, Clinique de St-Ame, Centre hospitalier du Valais romand, 1890 St-Maurice. FAU - Jean, Michele AU - Jean M AD - Service de geriatrie, Hopital de Martigny, Centre hospitalier du Valais romand, 1920 Martigny. FAU - Socasau, Camille AU - Socasau C AD - Service de geriatrie, Clinique de St-Ame, Centre hospitalier du Valais romand, 1890 St-Maurice. FAU - Moro, Davide AU - Moro D AD - Service de geriatrie, Hopital de Sierre, Centre hospitalier du Valais romand, 3960 Sierre. LA - fre PT - English Abstract PT - Journal Article TT - Recourir a la voie sous-cutanee en geriatrie : etat des lieux et recommandations. PL - Switzerland TA - Rev Med Suisse JT - Revue medicale suisse JID - 101219148 SB - IM MH - Humans MH - Injections, Subcutaneous MH - Aged MH - *Geriatrics MH - Practice Guidelines as Topic MH - Evidence-Based Medicine COIS- les auteur-es n'ont declare aucun conflit d'interets en relation avec cet article. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 04:33 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/10/30 04:33 [entrez] AID - RMS0981-003 [pii] AID - 10.53738/REVMED.2026.22.981.49160 [doi] PST - ppublish SO - Rev Med Suisse. 2026 Oct 28;22(981):1749-1753. doi: 10.53738/REVMED.2026.22.981.49160. PMID- 42958957 OWN - NLM STAT- Publisher LR - 20261030 IS - 1365-2036 (Electronic) IS - 0269-2813 (Linking) DP - 2026 Oct 30 TI - Letter: Temporal Misalignment and Selective Adjustment in PPI Risk Estimates After FMT. LID - 10.1111/apt.71084 [doi] FAU - Zhang, Zhanna AU - Zhang Z AUID- ORCID: 0009-0008-8520-0808 AD - Department of Hematology, The Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, China. FAU - Hu, Beili AU - Hu B AD - Department of Hematology, The Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, China. LA - eng PT - Letter DEP - 20261030 PL - England TA - Aliment Pharmacol Ther JT - Alimentary pharmacology & therapeutics JID - 8707234 SB - IM EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 04:13 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/10/25 00:00 [revised] PHST- 2026/10/25 00:00 [received] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/30 04:13 [entrez] AID - 10.1111/apt.71084 [doi] PST - aheadofprint SO - Aliment Pharmacol Ther. 2026 Oct 30. doi: 10.1111/apt.71084. PMID- 42958956 OWN - NLM STAT- Publisher LR - 20261030 IS - 1462-0332 (Electronic) IS - 1462-0324 (Linking) DP - 2026 Oct 29 TI - Serological and clinical outcomes of rituximab in antiphospholipid syndrome with or without SLE: a retrospective single-centre cohort. LID - keag609 [pii] LID - 10.1093/rheumatology/keag609 [doi] AB - OBJECTIVES: To assess the dynamics of aPL titres after rituximab treatment, durability of serological responses, recurrent thrombosis, and safety in patients with APS. METHODS: This retrospective study included 54 patients, predominantly with SLE-associated APS (n = 43), who received at least one course of rituximab. aCL and anti-beta2-glycoprotein I (abeta2GPI) IgG/IgM were evaluated at baseline and 6-12 months after treatment. Long-term serological, clinical and safety outcomes were analysed over a median follow-up period of 4.7 years. RESULTS: At 6-12 months, positivity rates decreased significantly for aCL IgG (59.3% to 24.1%), aCL IgM (24.1% to 5.6%), abeta2GPI IgG (42.6% to 16.7%), and abeta2GPI IgM (20.4% to 5.6%) (all P <0.05). Long-term serological data were available for 31 patients. At last follow-up, abeta2GPI IgG positivity remained lower than baseline (P = 0.039), whereas quantitative titres were no longer significantly different. Long-term serological changes did not differ according to rituximab cycle number. Thrombotic recurrence occurred in five patients, four of whom were either off anticoagulation or had subtherapeutic INR values. Fifteen patients developed bacterial infections, seven SARS-CoV-2 infections, and five patients died during follow-up, including one who died from sepsis. CONCLUSION: Rituximab was associated with marked short-term reductions in aPL titres and positivity, although quantitative suppression was not sustained long term. Changes in aCL/abeta2GPI did not consistently reflect thrombotic risk. These findings suggest that rituximab may be a valuable adjunctive therapy in selected patients with difficult-to-treat APS, although causality cannot be established from this retrospective study. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the British Society for Rheumatology. FAU - Uludag, Omer AU - Uludag O AUID- ORCID: 0000-0001-9928-7766 AD - Division of Rheumatology, Department of Internal Medicine, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. FAU - Kemec, Gamze AU - Kemec G AD - Division of Rheumatology, Department of Internal Medicine, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. FAU - Altin, Soner AU - Altin S AD - Department of Internal Medicine, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. FAU - Ince, Burak AU - Ince B AD - Division of Rheumatology, Department of Internal Medicine, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. FAU - Yalcinkaya, Yasemin AU - Yalcinkaya Y AD - Division of Rheumatology, Department of Internal Medicine, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. FAU - Kamali, Sevil AU - Kamali S AD - Division of Rheumatology, Department of Internal Medicine, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. FAU - Gul, Ahmet AU - Gul A AD - Division of Rheumatology, Department of Internal Medicine, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. FAU - Inanc, Murat AU - Inanc M AD - Division of Rheumatology, Department of Internal Medicine, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. FAU - Artim-Esen, Bahar AU - Artim-Esen B AD - Division of Rheumatology, Department of Internal Medicine, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Turkey. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Rheumatology (Oxford) JT - Rheumatology (Oxford, England) JID - 100883501 SB - IM OTO - NOTNLM OT - Antiphospholipid Syndrome OT - B-cell Targets OT - Rituximab OT - Systemic Lupus Erythematosus OT - Thrombosis EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 04:13 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/05/04 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/30 04:13 [entrez] AID - 9005079 [pii] AID - 10.1093/rheumatology/keag609 [doi] PST - aheadofprint SO - Rheumatology (Oxford). 2026 Oct 29:keag609. doi: 10.1093/rheumatology/keag609. PMID- 42958955 OWN - NLM STAT- Publisher LR - 20261030 IS - 1943-3530 (Electronic) IS - 0003-7028 (Linking) DP - 2026 Oct 30 TI - EXPRESS: Early Detection of Huanglongbing in Citrus Trees using Raw Raman Spectra and Deep Residual Networks. PG - 37028261506406 LID - 10.1177/00037028261506406 [doi] AB - Huanglongbing (HLB) is a devastating citrus disease that requires rapid, non-invasive detection methods, especially during its asymptomatic phase. In this study is evaluated the performance of machine learning models and with 1D ResNet-50 and ResNet-152 models were trained from scratch and via transfer learning, for HLB detection using Raman spectroscopy by comparing raw versus pre-processed spectral data to classify Raman spectra from control, symptomatic, and asymptomatic citrus leaves employing raw data and pre-processed spectral data.The ResNet-152 architecture, which was trained from scratch on raw spectra, exhibited superior performance in comparison to all other models, achieving a value of 0.836 +/- 0.025 in the balanced accuracy metric and an area under the ROC curve of 0.945 +/- 0.011. Additionally, it attained values of 0.848 +/- 0.034 for precision and 0.836 +/- 0.025 for recall. It is of particular significance that raw data, incorporating biological fluorescence, yielded superior diagnostic markers in comparison to pre-processed spectra, thereby significantly enhancing the identification of asymptomatic samples.In the context of machine learning models, Random Forest demonstrated optimal performance, attaining a balanced accuracy of 0.842 +/- 0.029, an F1-measure of 0.852 +/- 0.028, an area under the ROC curve of 0.957 +/- 0.003, and precision and recall values of 0.873 +/- 0.022 and 0.842 +/- 0.029, respectively when is evaluated with raw spectra. It is noteworthy that raw data, incorporating biological fluorescence, yielded superior diagnostic markers in comparison to pre-processed spectra. This finding significantly enhances the identification of asymptomatic samples.Machine learning and deep learning architectures trained on raw Raman signals provide a high-precision, non-invasive tool for early detection of HLB. These architectures are competitive with gold standard and superior to the metrics obtained by an expert in the field. This approach eliminates the need for complex signal pre-processing, facilitating the development of real-time, cost-effective diagnostic platforms for sustainable citrus management. FAU - Torres-Galvan, Juan Carlos AU - Torres-Galvan JC FAU - Ramirez-Elias, Miguel G AU - Ramirez-Elias MG FAU - Perez, Moises Roberto Vallejo AU - Perez MRV FAU - Contreras, Hugo Ricardo Navarro AU - Contreras HRN LA - eng PT - Journal Article DEP - 20261030 PL - United States TA - Appl Spectrosc JT - Applied spectroscopy JID - 0372406 SB - IM OTO - NOTNLM OT - Huanglongbing OT - Raman spectroscopy OT - Random Forest OT - ResNet152 OT - ResNet18 EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 04:13 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/10/30 04:13 [entrez] AID - 10.1177/00037028261506406 [doi] PST - aheadofprint SO - Appl Spectrosc. 2026 Oct 30:37028261506406. doi: 10.1177/00037028261506406. PMID- 42958947 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1365-2214 (Electronic) IS - 0305-1862 (Linking) VI - 52 IP - 6 DP - 2026 Nov TI - Parent's and Service Provider's Perceptions of Family Involvement in Inclusive Early Childhood Education: Adaptation and Psychometric Validation of the Arabic Family Involvement Questionnaire. LID - e70369 [pii] LID - 10.1111/cch.70369 [doi] AB - OBJECTIVES: This study aimed to adapt the Family Involvement Questionnaire (FIQ) into Arabic for use in the Saudi research context. The adaptation involved translating the instrument, producing the Arabic version (FIQ-A) and culturally modifying selected items. The study then examined the psychometric properties and suitability of the FIQ-A for exploring parents' and service providers' perceptions of family involvement in inclusive early childhood education settings in Saudi Arabia. MATERIALS AND METHODS: The FIQ-A instrument created for this study was administered to participants from inclusive early childhood settings in Riyadh. The sample consisted of 100 parents and 35 service providers. The instrument measured three dimensions of family involvement: school-based involvement, home-based involvement and home-school conferencing. Descriptive statistics and reliability analyses were conducted to evaluate the psychometric properties of the scale and participants' perceptions of family involvement. RESULTS: Cronbach's alpha coefficients were alpha = 0.73 for school-based involvement, alpha = 0.89 for home-based involvement and alpha = 0.86 for home-school conferencing, demonstrating acceptable to high reliability across the three dimensions. Parents reported higher levels of home-based activities (M = 3.43 vs. 2.64) and home-school conferencing (M = 3.40 vs. 2.66) compared with school-based involvement (M = 3.07 vs. 2.56) than service providers (all p < 0.001). CONCLUSION: The FIQ-A instrument demonstrated suitable psychometric properties, and the findings indicated that families were willing and prepared to engage in their children's education. Service providers were also ready to facilitate and support this engagement. Overall, these findings underscore the importance of strengthening multiple forms of family involvement to promote inclusive practices and support the participation of children with disabilities in high-quality early childhood education settings. CI - (c) 2026 The Author(s). Child: Care, Health and Development published by John Wiley & Sons Ltd. FAU - Alotaibi, Wadha AU - Alotaibi W AD - Assistant Professor at Special Education Department, Shaqra University, Riyadh, Saudi Arabia. FAU - O'Donovan, Mary-Ann AU - O'Donovan MA AD - Conjoint Associate Professor Public Health, Centre for Disability Studies, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia. FAU - Evans, David AU - Evans D AD - Professor, Special Education Designation Coordinator, Special and Inclusive Education, Faculty of Arts and Social Sciences, The University of Sydney, Sydney, Australia. LA - eng PT - Journal Article PT - Validation Study PL - England TA - Child Care Health Dev JT - Child: care, health and development JID - 7602632 SB - IM MH - Humans MH - Psychometrics MH - Male MH - Saudi Arabia MH - Female MH - Surveys and Questionnaires/standards MH - Reproducibility of Results MH - *Parents/psychology MH - Child, Preschool MH - Adult MH - *Mainstreaming, Education OTO - NOTNLM OT - FIQ OT - Saudi Arabia OT - family involvement questionnaire OT - inclusive early childhood education OT - psychometric validation OT - scale adaptation EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 04:02 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/09/21 00:00 [revised] PHST- 2025/09/25 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/30 04:02 [entrez] AID - e70369 [pii] AID - 10.1111/cch.70369 [doi] PST - ppublish SO - Child Care Health Dev. 2026 Nov;52(6):e70369. doi: 10.1111/cch.70369. PMID- 42958928 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1759-7714 (Electronic) IS - 1759-7706 (Linking) VI - 17 IP - 21 DP - 2026 Nov TI - Integrating Machine Learning to Identify Prognostic Subtypes and the Cisplatin Resistance Gene RRM2 in Lung Adenocarcinoma. LID - e70407 [pii] LID - 10.1111/1759-7714.70407 [doi] AB - Lung adenocarcinoma (LUAD) is a highly invasive malignancy associated with poor prognosis. However, reliable molecular subtypes and prognostic signatures for LUAD, particularly their associations with tumor immunity and treatment response, remain poorly characterized. This study aimed to identify distinct molecular subtypes and establish a robust prognostic signature for LUAD, while exploring potential therapeutic targets associated with cisplatin resistance. In this study, we integrated ten clustering algorithms with 101 machine learning methods to identify two distinct molecular subtypes of LUAD and established a robust consensus machine learning-derived signature (CMLS). Patients belonging to the Cluster A subtype exhibited a more favorable prognosis and an enhanced immune response. Furthermore, a CMLS model constructed from 19 genes exhibited strong predictive power. Individuals with high CMLS scores displayed reduced CD4 + T-cell infiltration, poorer survival outcomes, and diminished responses to immunotherapy, whereas those with the low CMLS scores showed improved clinical outcomes, better immunotherapy responsiveness, and features of an immunologically "hot" tumor phenotype. Among the identified genes, ribonucleotide reductase subunit M2 (RRM2) emerged as a core regulator associated with LUAD prognosis. RRM2 was markedly upregulated in lung cancer tissues and the LUAD cell lines and was associated with the cisplatin (DDP) resistance. Suppression of RRM2 expression significantly enhanced the cisplatin sensitivity of A549/DDP cells. In summary, our findings establish a molecular classifier that stratifies LUAD patients into distinct risk categories and highlight RRM2 as a promising biomarker and therapeutic target associated with cisplatin resistance. CI - (c) 2026 The Author(s). Thoracic Cancer published by John Wiley & Sons Australia, Ltd. FAU - Huang, Huirong AU - Huang H AUID- ORCID: 0000-0002-5935-8459 AD - Department of Pulmonary and Critical Care Medicine, the Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, Gansu Province, China. AD - Department of Thoracic Surgery, the Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, Gansu Province, China. FAU - Tao, Ting AU - Tao T AUID- ORCID: 0000-0002-3709-1722 AD - Department of Nephrology, The Affiliated Dazu's Hospital of Chongqing Medical University, Chongging, China. FAU - Li, Zhenqing AU - Li Z AUID- ORCID: 0000-0003-0173-3700 AD - Department of Thoracic Surgery, the Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, Gansu Province, China. FAU - Zhai, Kerong AU - Zhai K AUID- ORCID: 0000-0002-7800-4947 AD - Department of Thoracic Surgery, the Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, Gansu Province, China. FAU - Cui, Baiqiang AU - Cui B AUID- ORCID: 0009-0009-7965-4704 AD - Department of Thoracic Surgery, the Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, Gansu Province, China. FAU - Feng, Haiming AU - Feng H AUID- ORCID: 0000-0002-6345-6393 AD - Department of Thoracic Surgery, the Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, Gansu Province, China. FAU - Li, Peiqiang AU - Li P AUID- ORCID: 0000-0001-9433-6629 AD - Department of Genetics, School of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu Province, China. FAU - Li, Bin AU - Li B AUID- ORCID: 0009-0008-3791-0936 AD - Department of Thoracic Surgery, the Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, Gansu Province, China. LA - eng GR - CY2023-MS-B04/Cuiying Scientific and Technological Innovation Program of The Second Hospital & Clinical Medical School, Lanzhou University/ GR - 23JRRA1046/Gansu Provincial Natural Science Foundation of China/ PT - Journal Article PL - Singapore TA - Thorac Cancer JT - Thoracic cancer JID - 101531441 RN - Q20Q21Q62J (Cisplatin) RN - EC 1.17.4.1 (Ribonucleoside Diphosphate Reductase) RN - EC 1.17.4.- (ribonucleotide reductase M2) RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - *Cisplatin/pharmacology/therapeutic use MH - *Ribonucleoside Diphosphate Reductase/genetics/metabolism MH - Prognosis MH - *Adenocarcinoma of Lung/genetics/drug therapy/pathology MH - *Drug Resistance, Neoplasm/genetics MH - *Lung Neoplasms/genetics/drug therapy/pathology MH - *Machine Learning MH - Female MH - Biomarkers, Tumor/genetics MH - Male MH - Gene Expression Regulation, Neoplastic OTO - NOTNLM OT - Muti-omics OT - RRM2 OT - cisplatin resistance OT - lung adenocarcinoma OT - machine learning OT - molecular subtypes OT - prognosis EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 03:52 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/09/02 00:00 [revised] PHST- 2025/11/14 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/30 03:52 [entrez] AID - e70407 [pii] AID - 10.1111/1759-7714.70407 [doi] PST - ppublish SO - Thorac Cancer. 2026 Nov;17(21):e70407. doi: 10.1111/1759-7714.70407. PMID- 42958927 OWN - NLM STAT- Publisher LR - 20261030 IS - 1699-5848 (Electronic) IS - 0213-3911 (Linking) DP - 2026 Oct 30 TI - FOXD3 promotes the transcription of RGS13 to inhibit colorectal cancer malignant progression and CD8(+) T cell apoptosis. PG - 25165 LID - 10.14670/HH-25-165 [doi] AB - BACKGROUND: Regulator of G-protein signaling 13 (RGS13) plays a tumor suppressor role in a variety of cancers, but its expression, function, and regulatory mechanism in colorectal cancer (CRC) are still unclear. METHODS: RGS13 expression in CRC was verified by bioinformatics analysis (GEO, GEPIA, and TNMplot) and clinical samples (qRT-PCR and western blot). In vitro, the effects of RGS13 overexpression on CRC cell proliferation, angiogenesis, metastasis, and glycolysis were evaluated using colony formation, tube formation, Transwell, and wound healing assays, and a glycolysis detection kit. The effect of RGS13 on apoptosis of CD8(+) T cells was analyzed by a co-culture system and flow cytometry. In vivo, the tumor suppressive effect of RGS13 was verified by a xenograft tumor model in nude mice. Bioinformatics prediction, dual-luciferase reporter assay, and the rescue experiment were used to explore the transcriptional regulation of FOXD3 on RGS13. RESULTS: RGS13 was downregulated in CRC tissues and cells, and its low expression was associated with poor prognosis of patients with CRC. Overexpression of RGS13 significantly inhibited the proliferation, angiogenesis, invasion, migration, and glycolysis of CRC cells, as well as suppressed the apoptosis of CD8(+) T cells in the co-culture system. In vivo experiments confirmed that RGS13 overexpression reduced the growth of transplanted CRC tumors. FOXD3 directly bound to the promoter region of RGS13 to activate its transcription. In addition, FOXD3 played a role in inhibiting the malignant progression of CRC and CD8(+) T-cell apoptosis by upregulating RGS13. CONCLUSION: The FOXD3/RGS13 axis not only directly inhibits the malignant behavior of CRC cells but also represses CD8(+) T-cell apoptosis by regulating the tumor microenvironment. CI - (c)The Author(s) 2026. Open Access. This article is licensed under a Creative Commons CC-BY International License. FAU - Meng, Lei AU - Meng L AD - Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Xingtai Medical University, Xingtai, China. 19831919560@163.com. FAU - He, Xiaoqing AU - He X AD - Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Xingtai Medical University, Xingtai, China. FAU - Fan, Xiaoye AU - Fan X AD - Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Xingtai Medical University, Xingtai, China. FAU - Zheng, Song AU - Zheng S AD - Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Xingtai Medical University, Xingtai, China. FAU - Zhang, Ranxing AU - Zhang R AD - Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Xingtai Medical University, Xingtai, China. LA - eng PT - Journal Article DEP - 20261030 PL - Spain TA - Histol Histopathol JT - Histology and histopathology JID - 8609357 SB - IM EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 03:52 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/10/30 03:52 [entrez] AID - HH-25-165 [pii] AID - 10.14670/HH-25-165 [doi] PST - aheadofprint SO - Histol Histopathol. 2026 Oct 30:25165. doi: 10.14670/HH-25-165. PMID- 42958919 OWN - NLM STAT- Publisher LR - 20261030 IS - 1469-445X (Electronic) IS - 0958-0670 (Linking) DP - 2026 Oct 30 TI - Photobiomodulation as a contemporary antioxidant intervention in myocardial infarction. LID - 10.1113/EP093042 [doi] AB - Myocardial infarction (MI) triggers a complex cascade of structural and functional alterations that frequently culminate in adverse cardiac remodelling and heart failure. Central to this process is the excessive production of reactive oxygen species (ROS), which disrupt mitochondrial function, impair calcium handling, and activate pro-apoptotic and pro-fibrotic pathways. Photobiomodulation (PBM) has emerged as a non-invasive therapeutic approach capable of modulating several key biological processes involved in post-infarction remodelling. Preclinical studies consistently demonstrate that PBM reduces infarct size, attenuates ventricular dilation, and improves both systolic and diastolic function. Through the absorption of red to near-infrared light by mitochondrial chromophores, particularly cytochrome c oxidase, PBM enhances cellular bioenergetics, modulates ROS production and activates redox-sensitive signalling pathways. These effects promote a shift from detrimental oxidative stress toward controlled ROS signalling and, in turn, facilitate cytoprotective, anti-inflammatory and pro-angiogenic responses. In addition, PBM promotes the mobilization of progenitor cells, contributing to tissue repair and microvascular restoration. Although still limited, the available clinical evidence suggests that PBM may confer modest improvements in cardiac function. However, variability in treatment protocols, delivery methods and study designs currently precludes definitive conclusions regarding its clinical efficacy. In summary, PBM represents a promising strategy for modulating oxidative stress after MI, integrating metabolic, redox and signalling pathways to attenuate cardiac remodelling. Further well-designed clinical studies are required to establish standardized protocols and to clarify its therapeutic potential in cardiovascular medicine. CI - (c) 2026 The Author(s). Experimental Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society. FAU - Batista, Gabriel Matheus Da Silva AU - Batista GMDS AUID- ORCID: 0000-0002-1542-5242 AD - Programa de Pos-graduacao em Cardiologia, Universidade Federal de Sao Paulo, Sao Paulo, Brazil. FAU - Teixeira, Ighor Luiz Azevedo AU - Teixeira ILA AD - Programa de Pos-graduacao em Cardiologia, Universidade Federal de Sao Paulo, Sao Paulo, Brazil. FAU - Antonio, Ednei Luiz AU - Antonio EL AD - Programa de Pos-graduacao em Cardiologia, Universidade Federal de Sao Paulo, Sao Paulo, Brazil. FAU - Serra, Andrey Jorge AU - Serra AJ AUID- ORCID: 0000-0002-5407-8183 AD - Programa de Pos-graduacao em Cardiologia, Universidade Federal de Sao Paulo, Sao Paulo, Brazil. LA - eng GR - 300199/2025-2/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ PT - Journal Article PT - Review DEP - 20261030 PL - England TA - Exp Physiol JT - Experimental physiology JID - 9002940 SB - IM OTO - NOTNLM OT - heart failure OT - myocardial infarction OT - photobiomodulation EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 03:34 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/04/13 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/30 03:34 [entrez] AID - 10.1113/EP093042 [doi] PST - aheadofprint SO - Exp Physiol. 2026 Oct 30. doi: 10.1113/EP093042. PMID- 42958916 OWN - NLM STAT- Publisher LR - 20261030 IS - 1467-1107 (Electronic) IS - 1047-9511 (Linking) DP - 2026 Oct 30 TI - Analysis of early surgical outcomes in adolescent and adult patients undergoing repair for tetralogy of Fallot. PG - 1-6 LID - 10.1017/S1047951126124925 [doi] AB - BACKGROUND: Tetralogy of Fallot is the most common cyanotic CHD and is ideally repaired during infancy. However, delayed diagnosis and limited access to congenital cardiac care may result in presentation during adolescence or adulthood. This study evaluated outcomes of tetralogy of Fallot repair in patients aged >/= 13 years. PATIENTS AND METHODS: This retrospective cohort study included patients aged >/= 13 years undergoing primary tetralogy of Fallot repair between June 2016 and June 2025. Demographic, operative, and post-operative data, including early mortality and reintervention, were analysed. RESULTS: Ninety-four patients underwent repair at a median age of 15 years (interquartile range: 3) and weight of 36.5 kg (interquartile range: 12.2). Pre-operative coil embolisation of aortopulmonary collaterals was required in 26.6%. Mean cardiopulmonary bypass and aortic cross-clamp times were 114.8 +/- 49.8 and 82.4 +/- 35.6 minutes, respectively. Mean ventilation duration was 7.7 +/- 7.1 hours, ICU stay 3.9 +/- 1.0 days, and post-operative hospital stay 4.6 +/- 1.3 days. Transannular patch repair was performed in 65 patients (69%), while 29 (31%) underwent pulmonary valve-preserving repair, with comparable early outcomes. Thirty-day mortality was 1.06%, and reintervention occurred in 2.1% during a mean follow-up of 38.4 months. CONCLUSIONS: Primary repair of tetralogy of Fallot in late-presenting adolescents and adults can be performed with low early mortality and acceptable morbidity. Careful pre-operative assessment, including identification and management of significant aortopulmonary collaterals, and individualised right ventricular outflow tract reconstruction are important components of management. These findings support definitive repair in appropriately selected late-presenting patients. FAU - Das, Debasis AU - Das D AUID- ORCID: 0000-0001-8281-4400 AD - Cardiac Surgery, Narayana Superspeciality Hospital - Howrah, India. ROR: https://ror.org/05kx1ke03 FAU - Barsainyan, Kajal P AU - Barsainyan KP AD - Cardiac Surgery, Narayana Superspeciality Hospital - Howrah, India. ROR: https://ror.org/05kx1ke03 FAU - Sarkar, Tanulina AU - Sarkar T AD - Cardiac Surgery, Narayana Superspeciality Hospital - Howrah, India. ROR: https://ror.org/05kx1ke03 FAU - Malladi, Varsha AU - Malladi V AD - Cardiac Surgery, Narayana Superspeciality Hospital - Howrah, India. ROR: https://ror.org/05kx1ke03 FAU - Das, Shubhadeep AU - Das S AUID- ORCID: 0000-0003-1996-2800 AD - Pediatric Cardiac Intensive Care/ PICU, Narayana Superspeciality Hospital - Howrah, India. FAU - Sharma, Manish Kumar AU - Sharma MK AD - Cardiac Anesthesia, Narayana Superspeciality Hospital - Howrah, India. FAU - Gajpal, Shivani AU - Gajpal S AD - Cardiac Anesthesia, Narayana Superspeciality Hospital - Howrah, India. FAU - Chattopadhyay, Amitabha AU - Chattopadhyay A AUID- ORCID: 0000-0002-0236-8357 AD - Pediatric Cardiology, Narayana Superspeciality Hospital - Howrah, India. LA - eng PT - Journal Article DEP - 20261030 PL - England TA - Cardiol Young JT - Cardiology in the young JID - 9200019 SB - IM OTO - NOTNLM OT - adult OT - mortality OT - outcomes OT - surgery OT - tetralogy of Fallot EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 03:33 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/10/30 03:33 [entrez] AID - S1047951126124925 [pii] AID - 10.1017/S1047951126124925 [doi] PST - aheadofprint SO - Cardiol Young. 2026 Oct 30:1-6. doi: 10.1017/S1047951126124925. PMID- 42958913 OWN - NLM STAT- Publisher LR - 20261030 IS - 1759-6653 (Electronic) IS - 1759-6653 (Linking) DP - 2026 Oct 30 TI - Naturally arising de novo open reading frames as potential zinc chelators in Drosophila melanogaster. LID - evag274 [pii] LID - 10.1093/gbe/evag274 [doi] AB - A central open problem in the study of de novo gene origination is that the molecular mechanisms and functions driving the emergence of such evolutionarily young, de novo protein-coding genes remain poorly understood. Metal chelation, a simple, directly selectable activity that both requires no specific interaction partners and is also compatible with intrinsic disorder, is one possible function. This possibility was tested using sequence signatures in 7,849 transcriptionally supported, still-segregating Drosophila melanogaster de novo open reading frames. Interestingly, these new open reading frames (neORFs) are enriched for bis-histidine motifs at the metal-coordination-competent spacings H-x-H and H-x-x-x-H and show no enrichment at the incompatible even spacings relative to repeat-masked intergenic ORFs. Notably, these neORFs were also found to lack the C-x-x-C grammar of canonical metal-binding proteins. I report that this H-x-H bis-histidine signal is generated by translation of (CA)n microsatellites into His-Thr-His in Drosophila melanogaster, as evidenced by a CAC-codon bias within H-x-H motifs and a fourfold threonine enrichment at the central position. I propose that recurrent microsatellite expansion supplies Drosophila with a distributed, independently originated class of candidate metal-binding de novo peptides. I then trace one neORF (ZMEG) from conserved ancestral non-coding sequence to a transcribed, melanogaster-lineage open reading frame whose (CA)9-derived His run presents a candidate His32-His36 bis-histidine site. I propose that such de novo proteins may constitute a class of molecules united not by common descent but by their shared origin in evolvable repeat sequence, highlighting the importance of emergence bias in molecular evolution. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution. FAU - Lee, UnJin AU - Lee U AD - Laboratory of Evolutionary Genetics and Genomics, The Rockefeller University, New York, NY, 10065, USA. LA - eng PT - Journal Article DEP - 20261030 PL - England TA - Genome Biol Evol JT - Genome biology and evolution JID - 101509707 SB - IM OTO - NOTNLM OT - METTL9 post-translational regulation OT - bis-histidine metal chelation OT - de novo gene origination OT - microsatellite emergence bias and evolvability OT - zinc homeostasis in Drosophila EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 03:33 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/08/27 00:00 [received] PHST- 2026/10/20 00:00 [revised] PHST- 2026/10/30 03:33 [entrez] AID - 9005044 [pii] AID - 10.1093/gbe/evag274 [doi] PST - aheadofprint SO - Genome Biol Evol. 2026 Oct 30:evag274. doi: 10.1093/gbe/evag274. PMID- 42958909 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1365-313X (Electronic) IS - 0960-7412 (Linking) VI - 128 IP - 3 DP - 2026 Nov TI - Natural variation in the evolutionarily conserved F-box gene SDEF promotes seed dormancy in rice. LID - e71203 [pii] LID - 10.1111/tpj.71203 [doi] AB - Insufficient seed dormancy causes pre-harvest sprouting (PHS) and severe yield losses in rice; however, genetic resources with practical breeding value remain limited. Here, we isolated a major dormancy gene, SDEF, via map-based cloning using chromosomal single segment substitution lines (SSSLs), identifying a functional SNP (Arg-to-Trp) as the causal mutation. CRISPR/Cas9-mediated knockout of SDEF led to PHS susceptibility, while overexpression significantly enhanced dormancy. Importantly, SDEF enhanced seed dormancy without compromising grain yield in SSSLs, confirming it as a promising breeding target. Moreover, SDEF encodes an angiosperm-conserved, nuclear-localized F-box protein and is preferentially expressed in the embryo, with expression increasing during seed maturation and declining upon germination. Mechanistically, protein interaction assays confirmed that SDEF recruits OSK1, with the Trp-type SDEF(K) exhibiting a more stable interaction than the Arg-type SDEF(H). SDEF is induced by abscisic acid (ABA) and appears to promote seed dormancy through the ABA pathway by activating ABA signaling genes and repressing alpha-amylase genes, as evidenced by the reduced ABA sensitivity of sdef mutants. Additionally, haplotype analysis revealed that natural variation in SDEF is associated with divergent dormancy levels, and reduced nucleotide diversity in cultivated rice indicates that SDEF was selected during domestication. Collectively, our results provide insights into the molecular basis of SDEF-mediated dormancy, providing an ideal target for breeding PHS-resistant rice. CI - (c) 2026 The Author(s). The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd. FAU - Zhou, Yuliang AU - Zhou Y AUID- ORCID: 0000-0002-9211-6064 AD - Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. FAU - Wang, Yueping AU - Wang Y AUID- ORCID: 0009-0006-3267-0035 AD - Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. FAU - Zhao, Guangmiao AU - Zhao G AD - Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. FAU - Liu, Chunbao AU - Liu C AD - Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. FAU - Zeng, Ruizhen AU - Zeng R AD - Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. FAU - Liang, Wenxuan AU - Liang W AD - Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. FAU - Han, Ru AU - Han R AD - Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. FAU - Kong, Hui AU - Kong H AUID- ORCID: 0009-0003-6873-2160 AD - Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. FAU - Cao, Xiaofan AU - Cao X AD - Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. FAU - Li, Minshi AU - Li M AD - Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. FAU - Chen, Huhui AU - Chen H AD - Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210014, China. FAU - Tan, Bin AU - Tan B AD - Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. FAU - Liu, Hong AU - Liu H AD - Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. FAU - Xu, Zhenjiang AU - Xu Z AD - Guangdong Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China. LA - eng GR - 32472032/National Natural Science Foundation of China/ GR - 31771888/National Natural Science Foundation of China/ GR - 31271804/National Natural Science Foundation of China/ GR - 2021B10564001/The Double First-Class Discipline Promotion Project/ GR - 2022A1515010843/Natural Science Foundation of Guangdong Province/ PT - Journal Article PL - England TA - Plant J JT - The Plant journal : for cell and molecular biology JID - 9207397 RN - 0 (Plant Proteins) RN - 72S9A8J5GW (Abscisic Acid) RN - 0 (F-Box Proteins) SB - IM MH - *Oryza/genetics/physiology MH - *Plant Dormancy/genetics/physiology MH - *Plant Proteins/genetics/metabolism MH - Abscisic Acid/metabolism MH - *F-Box Proteins/genetics/metabolism MH - Seeds/genetics/physiology MH - Germination/genetics MH - Gene Expression Regulation, Plant MH - Plants, Genetically Modified MH - CRISPR-Cas Systems MH - Genetic Variation OTO - NOTNLM OT - ABA signaling OT - CRISPR/Cas9 OT - Oryza sativa OT - SCF complex OT - domestication OT - seed dormancy EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 03:32 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/08/29 00:00 [revised] PHST- 2026/05/19 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/30 03:32 [entrez] AID - e71203 [pii] AID - 10.1111/tpj.71203 [doi] PST - ppublish SO - Plant J. 2026 Nov;128(3):e71203. doi: 10.1111/tpj.71203. PMID- 42958905 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1440-1827 (Electronic) IS - 1320-5463 (Linking) VI - 76 IP - 10 DP - 2026 Oct TI - Association of CAF-Related Stromal Markers and IMP3 Expression With Lymph Node Metastasis in Early Colorectal Cancer. LID - e70202 [pii] LID - 10.1111/pin.70202 [doi] AB - Accurate risk stratification for lymph node metastasis (LNM) is needed after endoscopic resection of early-stage colorectal cancer (CRC). We explored the associations of cancer-associated fibroblast (CAF)-related markers and insulin-like growth factor 2 mRNA-binding protein 3 (IMP3) expression with LNM. We analyzed data from 155 patients with pT1 CRC who were treated by primary or additional surgery after endoscopic resection. A matched case-control cohort comprising 18 patients with LNM and 29 LNM-negative controls was immunohistochemically analyzed. The area fraction and staining intensity of podoplanin (D2-40), hydrogen peroxide-inducible clone-5 (Hic-5), alpha-smooth muscle actin (alphaSMA), and IMP3 were assessed using image analysis. We evaluated CAF-related stromal markers in the superficial, middle, and invasive front regions and IMP3 in tumor areas. Decreased D2-40 expression at the invasive front showed a nominal association with LNM. Associations between LNM and each of Hic-5, alphaSMA, and IMP3 were not statistically significant. These hypothesis-generating findings suggest an association between reduced invasive-front D2-40 expression and LNM in pT1 CRC. CI - (c) 2026 Japanese Society of Pathology and John Wiley & Sons Australia, Ltd. FAU - Takano, Yumika AU - Takano Y AUID- ORCID: 0009-0006-8193-3014 AD - Digestive Diseases Center, Gastroenterological Surgery, Showa Medical University Koto Toyosu Hospital, Koto-ku, Tokyo, Japan. AD - Department of Pathology, Showa Medical University Koto Toyosu Hospital, Koto-ku, Tokyo, Japan. FAU - Nagumo, Tasuku AU - Nagumo T AUID- ORCID: 0009-0001-3894-4969 AD - Department of Pathology, Showa Medical University Koto Toyosu Hospital, Koto-ku, Tokyo, Japan. FAU - Terunuma, Takuya AU - Terunuma T AUID- ORCID: 0009-0003-8175-8613 AD - Digestive Diseases Center, Gastroenterological Surgery, Showa Medical University Koto Toyosu Hospital, Koto-ku, Tokyo, Japan. AD - Department of Pathology, Showa Medical University School of Medicine, Shinagawa-ku, Tokyo, Japan. FAU - Tanabe, Taro AU - Tanabe T AUID- ORCID: 0000-0001-8128-5519 AD - Digestive Diseases Center, Gastroenterological Surgery, Showa Medical University Koto Toyosu Hospital, Koto-ku, Tokyo, Japan. FAU - Tanabe, Mayo AU - Tanabe M AUID- ORCID: 0000-0001-6126-5684 AD - Digestive Diseases Center, Gastroenterology, Showa Medical University Koto Toyosu Hospital, Koto-ku, Tokyo, Japan. FAU - Hobo, Takahiro AU - Hobo T AD - Digestive Diseases Center, Gastroenterological Surgery, Showa Medical University Koto Toyosu Hospital, Koto-ku, Tokyo, Japan. FAU - Uragami, Naoyuki AU - Uragami N AUID- ORCID: 0000-0003-2974-8250 AD - Digestive Diseases Center, Gastroenterology, Showa Medical University Koto Toyosu Hospital, Koto-ku, Tokyo, Japan. FAU - Yamochi, Toshiko AU - Yamochi T AUID- ORCID: 0009-0006-5610-752X AD - Department of Pathology, Showa Medical University School of Medicine, Shinagawa-ku, Tokyo, Japan. FAU - Kushima, Miki AU - Kushima M AUID- ORCID: 0000-0002-1642-0478 AD - Department of Pathology, Showa Medical University Koto Toyosu Hospital, Koto-ku, Tokyo, Japan. FAU - Yokoyama, Noboru AU - Yokoyama N AUID- ORCID: 0000-0003-1882-0018 AD - Digestive Diseases Center, Gastroenterological Surgery, Showa Medical University Koto Toyosu Hospital, Koto-ku, Tokyo, Japan. LA - eng PT - Journal Article PL - Australia TA - Pathol Int JT - Pathology international JID - 9431380 RN - 0 (Biomarkers, Tumor) RN - 0 (RNA-Binding Proteins) RN - 0 (IMP3 protein, human) RN - 0 (IGF2BP3 protein, human) RN - 0 (Ribonucleoproteins, Small Nucleolar) SB - IM MH - Humans MH - *Colorectal Neoplasms/pathology/metabolism MH - *Lymphatic Metastasis/pathology MH - Female MH - *Biomarkers, Tumor/metabolism/analysis MH - Male MH - Middle Aged MH - *RNA-Binding Proteins/metabolism MH - Aged MH - *Cancer-Associated Fibroblasts/pathology/metabolism MH - Case-Control Studies MH - Adult MH - Aged, 80 and over MH - Ribonucleoproteins, Small Nucleolar OTO - NOTNLM OT - D2-40 OT - Hic-5 OT - IMP3 OT - cancer-associated fibroblasts OT - lymph node metastasis OT - pT1 colorectal cancer EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 03:23 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/10/08 00:00 [revised] PHST- 2026/06/27 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/30 03:23 [entrez] AID - e70202 [pii] AID - 10.1111/pin.70202 [doi] PST - ppublish SO - Pathol Int. 2026 Oct;76(10):e70202. doi: 10.1111/pin.70202. PMID- 42958903 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1478-5153 (Electronic) IS - 1362-1017 (Linking) VI - 31 IP - 6 DP - 2026 Nov TI - The Experiences and Needs of Nursing Staff During Patients' ICU-To-Ward Transition: A Meta-Synthesis of Qualitative Studies. LID - e70762 [pii] LID - 10.1111/nicc.70762 [doi] AB - BACKGROUND: Intensive care unit (ICU) transitional care ensures continuous care for patients transferring from the ICU to general wards. Relevant qualitative studies show that nurses play a vital role here, so exploring their real experiences and needs is essential for nursing development. AIM: To summarise the current qualitative evidence on the experiences and needs of nursing staff during ICU transitional care. STUDY DESIGN: A meta-synthesis of qualitative studies retrieved through a search conducted in China National Knowledge Infrastructure (CNKI), Wanfang, VIP Database, Cochrane Library, PubMed, Web of Science, CINAHL and Embase from inception to June 2025. All studies that presented qualitative findings on the experiences, feelings and needs of nursing staff, based on participants' reports, were included. Data extracted included publication year, location, sample size, study design, data collection method, phenomenon of interest and all qualitative themes identified. FINDINGS: Thirteen studies were selected for inclusion, resulting in four synthesised findings: the practical challenges faced by nursing staff during the ICU transition period, their perceptions and attitudes towards transitional care, their supportive needs for transitional care and recommendations for improving transitional care. CONCLUSIONS: The provision of transitional care services by nursing staff is influenced by factors such as existing medical resources, psychological support, workflows and organisational structures. These can guide nursing administrators and healthcare institutions in addressing the occupational needs of nursing personnel, optimising transitional care interventions and promoting the high-quality development of transitional care practices. RELEVANCE TO CLINICAL PRACTICE: Despite cultural differences, the experiences and needs of nursing staff during the transition period in the ICU reflect commonalities across cultures. The review gives specific advice for ICU transition care, including establishing effective communication models, psychological support and professional training, establishing ICU liaison nurses and formulating standardised ICU transition procedures. CI - (c) 2026 British Association of Critical Care Nurses. FAU - Ge, Shuchang AU - Ge S AUID- ORCID: 0009-0004-0152-5770 AD - School of Nursing, Bengbu Medical University, Bengbu, China. FAU - Zhang, Xuekun AU - Zhang X AUID- ORCID: 0009-0006-9690-4742 AD - The First Affiliated Hospital of Bengbu Medical University, Bengbu, China. FAU - Wang, Ying AU - Wang Y AD - The First Affiliated Hospital of Bengbu Medical University, Bengbu, China. FAU - Yang, Congyan AU - Yang C AUID- ORCID: 0009-0002-1269-9704 AD - The First Affiliated Hospital of Bengbu Medical University, Bengbu, China. LA - eng PT - Journal Article PT - Review PL - England TA - Nurs Crit Care JT - Nursing in critical care JID - 9808649 SB - IM MH - Humans MH - Qualitative Research MH - *Intensive Care Units/organization & administration MH - *Nursing Staff, Hospital/psychology MH - Attitude of Health Personnel MH - *Transitional Care MH - Continuity of Patient Care MH - *Patient Transfer OTO - NOTNLM OT - critical care OT - intensive care units OT - nursing staff OT - qualitative study OT - transitional care EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 03:23 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/10/08 00:00 [revised] PHST- 2026/01/19 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/30 03:23 [entrez] AID - e70762 [pii] AID - 10.1111/nicc.70762 [doi] PST - ppublish SO - Nurs Crit Care. 2026 Nov;31(6):e70762. doi: 10.1111/nicc.70762. PMID- 42958870 OWN - NLM STAT- Publisher LR - 20261030 IS - 1744-7666 (Electronic) IS - 1465-6566 (Linking) DP - 2026 Oct 29 TI - Rusfertide for the treatment of polycythemia vera. LID - 10.1080/14656566.2026.2757984 [doi] AB - INTRODUCTION: The US Food and Drug Administration recently approved rusfertide, a hepcidin mimetic, for the treatment of patients with polycythemia vera (PV) whose disease is inadequately controlled with available therapies. AREAS COVERED: Hepcidin is a liver-produced hormone that regulates systemic iron availability. By restricting iron supply to erythroid progenitor cells in PV, hepcidin reduces red blood cell production and erythrocytosis. In the double-blind, placebo-controlled, phase 3 VERIFY study, clinical response during weeks 20-32 was achieved by 76.9% of patients receiving rusfertide compared with 32.9% receiving placebo, with marked reductions in phlebotomy requirements. Rusfertide is administered weekly with subcutaneous injections, with dose titration from 20 mg, to maintain hematocrit levels below 45%. The most common adverse events were injection-site reactions, anemia, and fatigue. A targeted literature search was conducted in PubMed/MEDLINE through October 2026, supplemented by ClinicalTrials.gov, regulatory documents, and abstracts from major hematology meetings. EXPERT OPINION: Rusfertide is the first of a novel class of agents targeting the hepcidin regulatory axis. Following its approval, it may become part of the therapeutic armamentarium for selected patients. Future studies are needed to confirm its efficacy and to further characterize its safety and its potential impact on thrombosis, clonal evolution, and disease progression. FAU - Vannucchi, Alessandro Maria AU - Vannucchi AM AD - Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy. AD - CRIMM & Hematology Unit, University Hospital Careggi, Florence, Italy. FAU - Boldrini, Valentina AU - Boldrini V AD - Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy. AD - CRIMM & Hematology Unit, University Hospital Careggi, Florence, Italy. FAU - Loscocco, Giuseppe Gaetano AU - Loscocco GG AD - Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy. AD - CRIMM & Hematology Unit, University Hospital Careggi, Florence, Italy. FAU - Capecchi, Giulio AU - Capecchi G AD - Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy. AD - CRIMM & Hematology Unit, University Hospital Careggi, Florence, Italy. FAU - Guglielmelli, Paola AU - Guglielmelli P AD - Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy. AD - CRIMM & Hematology Unit, University Hospital Careggi, Florence, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - Expert Opin Pharmacother JT - Expert opinion on pharmacotherapy JID - 100897346 SB - IM OTO - NOTNLM OT - hepcidin OT - iron OT - myeloproliferative neoplasms OT - polycythemia vera OT - rusfertide EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 03:03 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/30 03:03 [entrez] AID - 10.1080/14656566.2026.2757984 [doi] PST - aheadofprint SO - Expert Opin Pharmacother. 2026 Oct 29. doi: 10.1080/14656566.2026.2757984. PMID- 42958868 OWN - NLM STAT- Publisher LR - 20261030 IS - 2379-3694 (Electronic) IS - 2379-3694 (Linking) DP - 2026 Oct 30 TI - Dual-Modal Localized Surface Plasmon Resonance Biosensor for Rapid Bacterial Gram-Typing and Species Identification in Bronchoalveolar Lavage Fluid. LID - 10.1021/acssensors.6c04217 [doi] AB - Rapid pathogen identification is critical for timely antibiotic therapy in patients with pneumonia, yet current methods remain too slow or complex for routine clinical use. Here, we describe a localized surface plasmon resonance (LSPR)-based biosensor platform that detects Gram-positive and Gram-negative bacteria directly in bronchoalveolar lavage fluid (BALF) without nucleic acid extraction or amplification. Gold nano-islands (AuNIs) functionalized with antibodies against lipoteichoic acid (LTA) or lipopolysaccharide (LPS) enabled selective bacterial capture. The sensor achieved a refractive index resolution of 8.52 x 10-8 RIU with good reusability and dynamic range. Detection limits reached the concentration of 0.027 muM for purified LTA and 0.208 endotoxin units (EU) for LPS. When tested with Escherichia coli in artificial samples, the LPS sensor showed a reproducibility standard deviation (RSD) of 3.14%, detecting bacterial growth significantly earlier than conventional OD600 measurement. In 33 clinical BALF samples, sensor results correlated strongly with standard diagnostics (Pearson r = 0.963) and distinguished infected from noninfected cases using cutoff values of 0.060 rad (Gram-positive) and 0.083 rad (Gram-negative). The platform detects polymyxin B susceptibility 8 h earlier than conventional OD600 measurements, and a proof-of-concept sandwich immunoassay enables species-level discrimination between E. coli and Klebsiella pneumoniae. With excellent reproducibility (RSD = 3.14%), reusability over six cycles (84.21% +/- 2.03% signal retention), and four-week storage stability (98.63% +/- 5.42%), this LSPR platform offers a practical, rapid approach for frontline bacterial screening and accelerated antibiotic stewardship in pneumonia. CI - (c) 2026 American Chemical Society. FAU - Xu, Bin AU - Xu B AD - Innovative institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine , Jinan250300, China. FAU - Jing, Lihua AU - Jing L AD - Department of Cardiac and Vascular Ultrasound, Jinan Central Hospital, Jinan250013, China. FAU - Hou, Huinan AU - Hou H AD - Innovative institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine , Jinan250300, China. FAU - Sun, Lu AU - Sun L AD - Innovative institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine , Jinan250300, China. FAU - Yue, Haoran AU - Yue H AD - Innovative institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine , Jinan250300, China. FAU - Zhang, Zixuan AU - Zhang Z AD - Innovative institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine , Jinan250300, China. FAU - Gao, Wenhao AU - Gao W AD - Innovative institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine , Jinan250300, China. FAU - Sun, Dongfeng AU - Sun D AD - Department of Thoracic Surgery, Shandong Key Laboratory of Digital Diagnosis and Treatment of Thoracic Oncology, Shandong Engineering Research Center of Intelligent Surgery, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan250014, China. FAU - Xin, Junhong AU - Xin J AD - Innovative institute of Chinese Medicine and Pharmacy, Shandong University of Traditional Chinese Medicine , Jinan250300, China. FAU - Gui, Ting AU - Gui T AD - Medical College of Shandong University of Traditional Chinese Medicine , Jinan250300, China. FAU - Visentin, Michele AU - Visentin M AD - Department of Clinical Pharmacology and Toxicology, University Hospital Zurich, University of Zurich, Zurich8006, Switzerland. FAU - Chen, Qingfa AU - Chen Q AUID- ORCID: 0000-0002-3502-0234 AD - Basic Medicine Research Centre, Jinan Central Hospital, Jinan250013, China. FAU - Gai, Zhibo AU - Gai Z AD - Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan250300, China. AD - Key Laboratory of Traditional Chinese Medicine Classical Theory, Ministry of Education, Shandong University of Traditional Chinese Medicine, Jinan250300, China. LA - eng GR - ZR2025MS1345/Natural Science Foundation of Shandong Province/ PT - Journal Article DEP - 20261030 PL - United States TA - ACS Sens JT - ACS sensors JID - 101669031 SB - IM OTO - NOTNLM OT - bacterial detection OT - biosensor OT - bronchoalveolar lavage fluid (BALF) OT - localized surface plasmon resonance (LSPR) OT - pneumonia EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 03:03 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/09/04 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/30 03:03 [entrez] AID - 5461339 [pii] AID - 10.1021/acssensors.6c04217 [doi] PST - aheadofprint SO - ACS Sens. 2026 Oct 30. doi: 10.1021/acssensors.6c04217. PMID- 42958867 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2053-1095 (Electronic) IS - 2053-1095 (Linking) VI - 12 IP - 6 DP - 2026 Nov TI - Neglected but Not Harmless: Characterising Nonantibiotic Antimicrobial Use in Small Animal Practices in Colombia. LID - e71303 [pii] LID - 10.1002/vms3.71303 [doi] AB - BACKGROUND: Antimicrobial resistance (AMR) is a growing global threat, yet stewardship efforts have largely overlooked nonantibiotic antimicrobials (NAAs), despite their widespread use and potential role in resistance selection and environmental persistence. Evidence on NAA use in small animal practice, particularly in low- and middle-income countries, remains scarce. OBJECTIVE: To characterise patterns of NAA use and assess prescription quality in dogs, cats, and other small animal species treated at a university veterinary hospital in Colombia. METHODS: A retrospective cross-sectional study was conducted using veterinary prescription records from January to December 2022. A systematic sampling approach (first four prescriptions per day) yielded 1460 prescriptions, of which 731 involved NAAs. Data on animal characteristics, consultation type, and prescription details were extracted, and NAAs were classified by pharmacological group, pharmaceutical form, and route of administration. Bivariate analyses were performed to identify stewardship-relevant patterns. RESULTS: NAAs accounted for a substantial proportion of prescriptions, predominantly in dogs (91.5%). Topical antiseptics (49.9%), external antiparasitics (21.3%), and internal antiparasitics (17.1%) were the most frequently used classes. Cutaneous (58.7%) and oral (41.3%) routes predominated, with chlorhexidine as the most common active ingredient (22%). Dermatology consultations were more likely to involve topical antiseptics (55.0% vs. 45.5%; OR = 1.47; 95% CI 1.06-2.02), whereas general consultations showed substantially higher odds of internal antiparasitic use (OR = 5.58; 95% CI 2.93-10.62). While 67.6% of treatments adhered to standard duration, only 38% matched recommended dosing, and key prescription elements were frequently missing, including International Nonproprietary Names (7%), concentration (12.2%), and complete administration instructions (5.5%). CONCLUSIONS: NAAs are widely used in small animal practice but are frequently prescribed with incomplete documentation and suboptimal dosing practices. These findings highlight a critical and underrecognised gap in antimicrobial stewardship and support the need to formally integrate NAAs into surveillance, regulatory frameworks, and stewardship interventions under a One Health approach. CI - (c) 2026 The Author(s). Veterinary Medicine and Science published by John Wiley & Sons Ltd. FAU - Correa-Valencia, Nathalia M AU - Correa-Valencia NM AUID- ORCID: 0000-0001-8836-8827 AD - CENTAURO Research Group, Faculty of Agricultural Sciences, School of Veterinary Medicine, Universidad de Antioquia (UdeA), Medellin, Antioquia, Colombia. FAU - Arango-Arcila, Susana AU - Arango-Arcila S AUID- ORCID: 0009-0006-2153-2526 AD - Faculty of Agricultural Sciences, School of Veterinary Medicine, Universidad de Antioquia (UdeA), Medellin, Antioquia, Colombia. FAU - Mendoza, Nicolle Kamel AU - Mendoza NK AUID- ORCID: 0009-0003-2566-5295 AD - Faculty of Agricultural Sciences, School of Veterinary Medicine, Universidad de Antioquia (UdeA), Medellin, Antioquia, Colombia. FAU - Sierra-Zapata, Diego Alejandro AU - Sierra-Zapata DA AUID- ORCID: 0009-0008-4851-7080 AD - Faculty of Agricultural Sciences, School of Veterinary Medicine, Universidad de Antioquia (UdeA), Medellin, Antioquia, Colombia. FAU - Guzman-Mejia, Victoria A AU - Guzman-Mejia VA AUID- ORCID: 0009-0003-5613-1202 AD - Faculty of Agricultural Sciences, School of Veterinary Medicine, Universidad de Antioquia (UdeA), Medellin, Antioquia, Colombia. FAU - Carmona Ramirez, Alejandra AU - Carmona Ramirez A AUID- ORCID: 0009-0008-1302-7591 AD - Faculty of Agricultural Sciences, School of Veterinary Medicine, Universidad de Antioquia (UdeA), Medellin, Antioquia, Colombia. FAU - Restrepo Salazar, Juan Gonzalo AU - Restrepo Salazar JG AUID- ORCID: 0000-0003-1645-6558 AD - CENTAURO Research Group, Faculty of Agricultural Sciences, School of Veterinary Medicine, Universidad de Antioquia (UdeA), Medellin, Antioquia, Colombia. LA - eng PT - Journal Article PL - England TA - Vet Med Sci JT - Veterinary medicine and science JID - 101678837 RN - 0 (Anti-Infective Agents) SB - IM MH - Animals MH - Colombia MH - Dogs MH - Cross-Sectional Studies MH - Retrospective Studies MH - Cats MH - *Anti-Infective Agents/therapeutic use MH - *Antimicrobial Stewardship/statistics & numerical data MH - Dog Diseases/drug therapy MH - Hospitals, Animal/statistics & numerical data OTO - NOTNLM OT - Colombia OT - One Health OT - antimicrobial stewardship OT - nonantibiotic antimicrobials OT - small animal practice OT - veterinary prescribing EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 03:03 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/08/31 00:00 [revised] PHST- 2025/12/28 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/30 03:03 [entrez] AID - e71303 [pii] AID - 10.1002/vms3.71303 [doi] PST - ppublish SO - Vet Med Sci. 2026 Nov;12(6):e71303. doi: 10.1002/vms3.71303. PMID- 42958856 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 2288-5919 (Print) IS - 2288-5919 (Linking) VI - 45 IP - 6 DP - 2026 Nov TI - Ultrasound-guided thermal ablation for T1b and selected T2 papillary thyroid carcinoma: current evidence, evolving guideline recommendations, and an ultrasound-based clinical workflow. PG - 529-548 LID - 10.14366/usg.26199 [doi] AB - Thermal ablation (TA) is established for benign thyroid nodules, recurrent thyroid carcinoma, and low-risk papillary thyroid microcarcinoma. Its role in papillary thyroid carcinoma (PTC) remains unsettled. This review examines evidence and guideline recommendations for TA in T1b and selected T2 PTC. T1b studies suggest encouraging local control and low permanent morbidity, but retrospective, geographically concentrated, heterogeneous evidence leaves surgery as the reference treatment. Four retrospective T2 reports indicate a narrower procedural safety margin. The same anatomical selection criteria apply across T1a, T1b, and T2 disease; however, as tumors enlarge, fewer patients meet oncological and technical requirements, and incomplete staging or margins have greater consequences. FAU - Lu, Chia Yin AU - Lu CY AD - Department of Diagnostic Radiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan. AD - Department of Diagnostic Radiology, Kaohsiung Municipal Fong Shan Hospital-under the management of Chang Gung Medical Foundation, Kaohsiung, Taiwan. AD - Department of Diagnostic Radiology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan. AD - Thyroid Head and Neck Ablation Center, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan. FAU - Wang, Cheng-Kang AU - Wang CK AD - Department of Diagnostic Radiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan. AD - Department of Diagnostic Radiology, Kaohsiung Municipal Fong Shan Hospital-under the management of Chang Gung Medical Foundation, Kaohsiung, Taiwan. AD - Department of Diagnostic Radiology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan. AD - Thyroid Head and Neck Ablation Center, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan. AD - School of Medicine and Doctoral Program of Clinical and Experimental Medicine, College of Medicine, National Sun Yat-sen University, Kaohsiung, Taiwan. AD - Department of Diagnostic Radiology, Jen-Ai Hospital, Taichung, Taiwan. FAU - Chang-Chien, Guo-Ping AU - Chang-Chien GP AD - Center for Environmental Toxin and Emerging-Contaminant Research, Cheng Shiu University, Kaohsiung, Taiwan. AD - Super Micro Mass Research and Technology Center, Cheng Shiu University, Kaohsiung, Taiwan. AD - Institute of Environmental Toxin and Emerging-Contaminant Research, Cheng Shiu University, Kaohsiung, Taiwan. FAU - Lin, Wei-Che AU - Lin WC AD - Department of Diagnostic Radiology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan. AD - Department of Diagnostic Radiology, Kaohsiung Municipal Fong Shan Hospital-under the management of Chang Gung Medical Foundation, Kaohsiung, Taiwan. AD - Department of Diagnostic Radiology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan. AD - Thyroid Head and Neck Ablation Center, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan. AD - School of Medicine, College of Medicine, National Sun Yat-sen University, Kaohsiung, Taiwan. LA - eng PT - Journal Article DEP - 20261027 PL - Korea (South) TA - Ultrasonography JT - Ultrasonography (Seoul, Korea) JID - 101626019 OTO - NOTNLM OT - Microwave ablation OT - Papillary thyroid carcinoma OT - Radiofrequency ablation OT - Thermal ablation OT - Thyroid nodule EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 03:00 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/06/26 00:00 [received] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/30 03:00 [entrez] AID - usg.26199 [pii] AID - 10.14366/usg.26199 [doi] PST - ppublish SO - Ultrasonography. 2026 Nov;45(6):529-548. doi: 10.14366/usg.26199. Epub 2026 Oct 27. PMID- 42958853 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1439-0507 (Electronic) IS - 0933-7407 (Linking) VI - 69 IP - 11 DP - 2026 Nov TI - Azole-Resistant Aspergillosis: An Emerging Diagnostic and Therapeutic Challenge in Paediatrics. LID - e70206 [pii] LID - 10.1111/myc.70206 [doi] AB - INTRODUCTION: Invasive aspergillosis (IA) represents one of the most frequent invasive fungal infections in paediatric patients with risk factors. An increase in cases of azole-resistant IA has been reported in adult patients. The aim of this study is to present a series of 11 cases of paediatric patients with this pathology. METHODS: A retrospective descriptive study including paediatric patients with probable or proven azole-resistant IA treated between January 2019 and June 2024 in four tertiary hospitals in Spain. RESULTS: Eleven cases are reported, all patients with risk factors for IA, mainly with haematopoietic stem cell transplantation (HCT) due to haematological malignancies and heart transplant in the early post-transplant period. These patients represent 22% of all proven or probable paediatric IA cases (n = 50) diagnosed in the study centres during the period. Five were previously receiving azole-based antifungal prophylaxis. The most frequently isolated species was Aspergillus fumigatus. Despite receiving at least partially active antifungals plus adjunctive treatment therapy from diagnosis, attributable mortality was high (8/11 patients). CONCLUSION: Azole-resistant IA should be suspected in paediatric patients with risk factors, especially if they show poor clinical course or have received azole prophylaxis. The performance of invasive tests, as well as species identification and resistance testing, is essential to optimise treatment. In breakthrough IA it should be considered empirical treatment with an antifungal from a different class than the one used for prophylaxis, alone or in combination. CI - (c) 2026 The Author(s). Mycoses published by Wiley-VCH GmbH. FAU - Mendoza-Palomar, Natalia AU - Mendoza-Palomar N AUID- ORCID: 0000-0002-2035-8291 AD - Paediatric Infectious Diseases and Immunodeficiencies Unit, Children's Hospital, Vall D'hebron Barcelona Hospital Campus, Barcelona, Catalonia, Spain. AD - Infection and Immunity in Children, Vall D'hebron Institut de Recerca (VHIR), Vall D'hebron Barcelona Hospital Campus, Barcelona, Spain. FAU - Grasa Lozano, Carlos AU - Grasa Lozano C AUID- ORCID: 0000-0002-9323-2024 AD - Paediatric Infectious Diseases Department, Hospital Universitario La Paz (Madrid), Madrid, Spain. AD - Instituto de Investigacion del Hospital Universitario La Paz (IdiPAZ), Madrid, Spain. AD - Centro de Investigacion en Red en Enfermedades Infecciosas (CIBERINFEC), Madrid, Spain. FAU - Carazo-Gallego, Begona AU - Carazo-Gallego B AUID- ORCID: 0000-0003-4807-436X AD - Paediatric Infectious Diseases and Immunodeficiencies, Malaga Regional University Hospital, Malaga, Spain. FAU - Rincon-Lopez, Elena Maria AU - Rincon-Lopez EM AUID- ORCID: 0000-0002-0982-1636 AD - Paediatric Infectious Diseases Unit, Gregorio Maranon General Hospital, Madrid, Spain. AD - Centro de Investigacion Biomedica en Red del Instituto de Salud Carlos III (CIBERINFEC), Madrid, Spain. AD - Instituto de Investigacion Sanitaria Gregorio Maranon (IISGM), Madrid, Spain. FAU - Pinyol-Sarrias, Maria AU - Pinyol-Sarrias M AUID- ORCID: 0009-0007-5643-507X AD - Paediatric Infectious Diseases and Immunodeficiencies Unit, Children's Hospital, Vall D'hebron Barcelona Hospital Campus, Barcelona, Catalonia, Spain. AD - Infection and Immunity in Children, Vall D'hebron Institut de Recerca (VHIR), Vall D'hebron Barcelona Hospital Campus, Barcelona, Spain. FAU - Creus-Costa, Anna AU - Creus-Costa A AUID- ORCID: 0000-0002-9861-5550 AD - Paediatric Infectious Diseases and Immunodeficiencies Unit, Children's Hospital, Vall D'hebron Barcelona Hospital Campus, Barcelona, Catalonia, Spain. AD - Infection and Immunity in Children, Vall D'hebron Institut de Recerca (VHIR), Vall D'hebron Barcelona Hospital Campus, Barcelona, Spain. FAU - Mendez-Echevarria, Ana AU - Mendez-Echevarria A AUID- ORCID: 0000-0002-7455-9080 AD - Paediatric Infectious Diseases Department, Hospital Universitario La Paz (Madrid), Madrid, Spain. AD - Instituto de Investigacion del Hospital Universitario La Paz (IdiPAZ), Madrid, Spain. AD - Centro de Investigacion en Red en Enfermedades Infecciosas (CIBERINFEC), Madrid, Spain. FAU - Maldonado-Barrueco, Alfredo AU - Maldonado-Barrueco A AUID- ORCID: 0000-0002-1856-1725 AD - Instituto de Investigacion del Hospital Universitario La Paz (IdiPAZ), Madrid, Spain. AD - Centro de Investigacion en Red en Enfermedades Infecciosas (CIBERINFEC), Madrid, Spain. AD - Clinical Microbiology Department, Hospital Universitario La Paz (Madrid), Madrid, Spain. FAU - Mozo, Yasmina AU - Mozo Y AUID- ORCID: 0000-0002-5666-1911 AD - Paediatric Hemato-Oncology Department, Hospital Universitario La Paz (Madrid), Madrid, Spain. FAU - Lozano-Duran, Diego AU - Lozano-Duran D AD - Paediatric Department, Hospital Costa del Sol, Malaga, Spain. FAU - Navarro Gomez, Maria Luisa AU - Navarro Gomez ML AUID- ORCID: 0000-0002-1234-6936 AD - Paediatric Infectious Diseases Unit, Gregorio Maranon General Hospital, Madrid, Spain. AD - Centro de Investigacion Biomedica en Red del Instituto de Salud Carlos III (CIBERINFEC), Madrid, Spain. AD - Instituto de Investigacion Sanitaria Gregorio Maranon (IISGM), Madrid, Spain. AD - Universidad Complutense de Madrid (UCM), Madrid, Spain. FAU - Guinea, Jesus AU - Guinea J AUID- ORCID: 0000-0002-7901-8355 AD - Instituto de Investigacion Sanitaria Gregorio Maranon (IISGM), Madrid, Spain. AD - Clinical Microbiology and Infectious Diseases Department, Hospital General Universitario Gregorio Maranon, Madrid, Spain. AD - Faculty of Health Sciences - HM Hospitals, Universidad Camilo Jose Cela, Madrid, Spain. AD - CIBER Enfermedades Respiratorias-CIBERES (CB06/06/0058), Madrid, Spain. FAU - Mediavilla Gradolph, Maria Concepcion AU - Mediavilla Gradolph MC AUID- ORCID: 0000-0001-7660-4386 AD - Microbiology Department, Malaga Regional University Hospital, Malaga, Spain. FAU - Soler-Palacin, Pere AU - Soler-Palacin P AUID- ORCID: 0000-0002-0346-5570 AD - Paediatric Infectious Diseases and Immunodeficiencies Unit, Children's Hospital, Vall D'hebron Barcelona Hospital Campus, Barcelona, Catalonia, Spain. AD - Infection and Immunity in Children, Vall D'hebron Institut de Recerca (VHIR), Vall D'hebron Barcelona Hospital Campus, Barcelona, Spain. AD - Universitat Autonoma de Barcelona (UAB), Bellaterra, Catalonia, Spain. FAU - Martin-Gomez, Maria Teresa AU - Martin-Gomez MT AUID- ORCID: 0000-0001-7679-1054 AD - Microbiology Department, Vall D'hebron Hospital Campus, Barcelona, Catalonia, Spain. AD - Microbiology, Vall D'hebron Institut de Recerca (VHIR), Vall D'hebron Barcelona Hospital Campus, Barcelona, Spain. LA - eng GR - Gilead Sciences/ PT - Journal Article PL - Germany TA - Mycoses JT - Mycoses JID - 8805008 RN - 0 (Antifungal Agents) RN - 0 (Azoles) SB - IM MH - Humans MH - *Antifungal Agents/therapeutic use/pharmacology MH - *Azoles/pharmacology/therapeutic use MH - Retrospective Studies MH - *Drug Resistance, Fungal MH - Female MH - Male MH - Child MH - Spain MH - Child, Preschool MH - Aspergillus fumigatus/drug effects/isolation & purification MH - Risk Factors MH - Adolescent MH - *Aspergillosis/drug therapy/diagnosis MH - Hematopoietic Stem Cell Transplantation/adverse effects MH - Infant OTO - NOTNLM OT - fungal drug resistance OT - invasive pulmonary aspergillosis OT - paediatrics EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 02:53 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/06/10 00:00 [revised] PHST- 2026/02/11 00:00 [received] PHST- 2026/06/19 00:00 [accepted] PHST- 2026/10/30 02:53 [entrez] AID - e70206 [pii] AID - 10.1111/myc.70206 [doi] PST - ppublish SO - Mycoses. 2026 Nov;69(11):e70206. doi: 10.1111/myc.70206. PMID- 42958850 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1875-5704 (Electronic) IS - 1567-2018 (Linking) VI - 23 IP - 8 DP - 2026 TI - Enhancing Solubility and alpha-Glucosidase Inhibition of Mangosteen Peel Extract via Amorphous Solid Dispersion: A Comparative Study of Solvent Evaporation and Fluidized Bed Drying Methods. PG - 984-995 LID - 10.2174/0115672018422136260130000617 [doi] AB - INTRODUCTION: Type 2 diabetes mellitus represents a growing global health burden, with current alpha-glucosidase inhibitors such as acarbose and miglitol demonstrating limited efficacy and notable gastrointestinal side effects. alpha-Mangostin, a bioactive compound from mangosteen peel, shows promise as a natural alpha-glucosidase inhibitor but suffers from poor aqueous solubility. This study aimed to enhance the solubility and enzyme inhibitory activity of mangosteen peel extract through amorphous solid dispersion using two manufacturing approaches: solvent evaporation and fluidized bed drying. METHODS: Mangosteen peel extract was formulated into amorphous solid dispersions using polyvinylpyrrolidone K30 at a 1:3 polymer ratio via solvent evaporation and fluidized bed drying methods. Solubility enhancement was evaluated under standardized conditions, and alpha-glucosidase inhibitory activity was assessed through IC50 determination. Comparative analysis against acarbose and quercetin standards was performed. RESULTS: Solvent evaporation achieved 2.1-fold solubility enhancement, while fluidized bed drying yielded 1.8-fold improvement. The mangosteen peel extract demonstrated superior alpha-glucosidase inhibition (IC(50) = 20.13 +/- 0.02 mug/mL) compared to acarbose (IC(50) = 379.75 +/- 0.57 mug/mL) but remained less potent than quercetin (IC(50) = 2.97 +/- 0.05 mug/mL). When normalized to equivalent alpha- mangostin concentrations, fluidized bed drying preserved inhibitory activity more effectively (IC(50) = 112.87 +/- 0.06 mug/mL) than solvent evaporation (IC50 = 1238.48 +/- 0.51 mug/mL). DISCUSSION: The manufacturing method significantly influenced both solubility and biological activity. Solvent evaporation maximized solubility enhancement but compromised enzyme inhibitory potency, whereas fluidized bed drying maintained superior inhibitory activity despite moderate solubility improvement. This trade-off suggests that formulation strategy selection should prioritize the desired therapeutic outcome. CONCLUSION: Amorphous solid dispersion successfully enhanced both solubility and alpha-glucosidase inhibitory activity of mangosteen peel extract. Fluidized bed drying represents the optimal manufacturing approach for preserving enzyme inhibition while achieving meaningful solubility enhancement, positioning alpha-mangostin as a promising candidate for natural antidiabetic drug development. CI - Copyright(c) Bentham Science Publishers; For any queries, please email at epub@benthamscience.net. FAU - Maulana, Ahmad Fariz AU - Maulana AF AD - Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang, Bandung, 45363, Indonesia. FAU - Aisyah, Ranya Nur AU - Aisyah RN AD - Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang, Bandung, 45363, Indonesia. FAU - Sriwidodo, Sriwidodo AU - Sriwidodo S AD - Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Padjadjaran, Bandung, 45363, Indonesia. FAU - Maksum, Iman Permana AU - Maksum IP AUID- ORCID: 0000-0001-8166-8421 AD - Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang, Bandung, 45363, Indonesia. FAU - Rukayadi, Yaya AU - Rukayadi Y AD - Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. LA - eng PT - Comparative Study PT - Journal Article PL - United Arab Emirates TA - Curr Drug Deliv JT - Current drug delivery JID - 101208455 RN - 0 (Glycoside Hydrolase Inhibitors) RN - 0 (Solvents) RN - 0 (Plant Extracts) RN - EC 3.2.1.20 (alpha-Glucosidases) RN - U6RIV93RU1 (mangostin) RN - 0 (Xanthones) RN - T58MSI464G (Acarbose) RN - FZ989GH94E (Povidone) SB - IM MH - *Glycoside Hydrolase Inhibitors/chemistry/pharmacology MH - Solubility MH - *Garcinia mangostana/chemistry MH - Solvents/chemistry MH - *Plant Extracts/chemistry/pharmacology MH - alpha-Glucosidases/metabolism MH - *Xanthones/chemistry MH - Acarbose/chemistry MH - Povidone/chemistry MH - Desiccation OTO - NOTNLM OT - and solvent evaporation OT - bioavailability OT - fluidized bed drying (FBD) OT - formulation strategy OT - alpha-glucosidase inhibition OT - alpha-mangostin EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 02:53 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2025/06/21 00:00 [received] PHST- 2025/10/19 00:00 [revised] PHST- 2025/10/31 00:00 [accepted] PHST- 2026/10/30 02:53 [entrez] AID - CDD-EPUB-154758 [pii] AID - 10.2174/0115672018422136260130000617 [doi] PST - ppublish SO - Curr Drug Deliv. 2026;23(8):984-995. doi: 10.2174/0115672018422136260130000617. PMID- 42958847 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1875-6220 (Electronic) IS - 1570-1638 (Linking) VI - 23 IP - 5 DP - 2026 TI - Artificial Intelligence-powered Advances in Phytomedicine: A Systematic Review on Plant-based Drug Discovery Software. PG - 56-80 LID - 10.2174/0115701638388155251020065128 [doi] AB - INTRODUCTION: Herbal medicines contain complex phytochemicals that offer therapeutic potential, but their long-term adverse effects remain challenging to determine. Traditional herbal medicine preparation methods are often labor-intensive, non-standardized, and face difficulties in identifying active ingredients. By improving medication development, optimization, and discovery procedures, Artificial Intelligence (AI) provides innovative ways to get beyond these restrictions. This systematic review aims to evaluate the role of AI-based software such as LEAFSNAP, Deep- Chem, PlantNet, Autodock, MestReNova, XCMS, ADMETlab, SynergyFinder, KEGG, TCMID, and Reactome in enhancing phytomedicine research by streamlining plant identification, bioactive compound discovery, molecular modeling, and pharmacokinetic profiling, thereby advancing drug discovery and development processes. METHODS: A systematic review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA guidelines) to explore the integration of AI-based tools in phytomedicine research and drug discovery. Literature searches were performed in Scopus, PubMed, and Google Scholar databases, focusing on studies published between January 2015 and January 2025. Only English-language articles focusing on AI-based software for drug discovery from plant-derived compounds were reviewed. Studies discussing synthetic drug development were excluded; this, along with non-English language, acted as a literature review limitation. Two reviewers independently screened the titles, abstracts, and full texts of the retrieved articles. Any discrepancies in the screening, study selection, and data extraction processes were resolved through discussion and consensus. RESULTS: Through the effective identification of bioactive chemicals and the simplification of intricate studies, the combination of artificial intelligence and computer tools has completely transformed the process of discovering and developing herbal drugs. DISCUSSION: Artificial intelligence tools like ADMETlab for pharmacokinetics and toxicity assessments, DeepChem for molecular activity modelling, and AlphaFold for protein structure prediction have greatly improved the search for new herbal drugs. While AutoDock improves molecular docking simulations by automating ligand-receptor binding predictions for bioactive chemicals, LeafSnap and PlantNet make plant identification simpler. Drug development workflows are improved by databases such as KEGG and TCMID, which combine pathway analysis with conventional medical knowledge. CONCLUSION: Research on phytomedicine may reach its full potential if AI techniques are improved and traditional knowledge and contemporary technology are better integrated. CI - Copyright(c) Bentham Science Publishers; For any queries, please email at epub@benthamscience.net. FAU - Vig, Himangi AU - Vig H AD - Department of Pharmacy, PSIT-Pranveer Singh Institute of (Pharmacy) NH 19, Kanpur Agra Highway, Bhauti, Kanpur, India. FAU - Wal, Ankita AU - Wal A AUID- ORCID: 0000-0002-5563-1382 AD - Department of Pharmacy, PSIT-Pranveer Singh Institute of (Pharmacy) NH 19, Kanpur Agra Highway, Bhauti, Kanpur, India. FAU - Khanam, Zia AU - Khanam Z AD - Department of Pharmacy, PSIT-Pranveer Singh Institute of (Pharmacy) NH 19, Kanpur Agra Highway, Bhauti, Kanpur, India. FAU - Nigam, Aditi AU - Nigam A AD - Department of Pharmacy, PSIT-Pranveer Singh Institute of (Pharmacy) NH 19, Kanpur Agra Highway, Bhauti, Kanpur, India. FAU - Pandey, Virat Kumar AU - Pandey VK AD - Department of Pharmacy, PSIT-Pranveer Singh Institute of (Pharmacy) NH 19, Kanpur Agra Highway, Bhauti, Kanpur, India. LA - eng PT - Journal Article PT - Systematic Review PL - United Arab Emirates TA - Curr Drug Discov Technol JT - Current drug discovery technologies JID - 101157212 RN - 0 (Phytochemicals) SB - IM MH - *Artificial Intelligence MH - *Drug Discovery/methods MH - Humans MH - *Software MH - Plants, Medicinal/chemistry MH - *Phytochemicals/chemistry MH - *Phytotherapy/methods OTO - NOTNLM OT - Autodock OT - DeepChem OT - LeafSnap OT - Phytomedicine OT - SynergyFinder OT - artificial intelligence OT - drug discovery EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 02:53 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2025/01/24 00:00 [received] PHST- 2025/04/07 00:00 [revised] PHST- 2025/04/21 00:00 [accepted] PHST- 2026/10/30 02:53 [entrez] AID - CDDT-EPUB-151785 [pii] AID - 10.2174/0115701638388155251020065128 [doi] PST - ppublish SO - Curr Drug Discov Technol. 2026;23(5):56-80. doi: 10.2174/0115701638388155251020065128. PMID- 42958840 OWN - NLM STAT- Publisher LR - 20261030 IS - 1521-3773 (Electronic) IS - 1433-7851 (Linking) DP - 2026 Oct 29 TI - Meta-Selective C horizontal line H Monofluoroalkylation of Pyridines With Difluoroalkyl Radicals. LID - e1059611 [pii] LID - 10.1002/anie.1059611 [doi] AB - C horizontal line H monofluoroalkylation of pyridines is a highly desirable transformation in medicinal chemistry because the monofluoroalkyl group is known to impart favorable properties to drug candidates, and pyridine rings are prevalent in drug molecules. However, only a few methods have been reported, and they are limited to ortho-selective reactions. Herein, we report an unprecedented method for meta-selective C horizontal line H monofluoroalkylation of pyridines via reactions between difluoroalkyl radicals and dihydropyridines generated in situ. During the reactions, a defluorination/olefin isomerization cascade converts initially formed difluoroalkylated dihydropyridines to monofluoroalkylated pyridines. The method accommodates a broad range of pyridine substrates, as well as other N-heteroarenes and fluoroalkylating reagents, and shows promise for late-stage functionalization of pyridine-containing drugs. CI - (c) 2026 Wiley-VCH GmbH. FAU - Zhang, Ming AU - Zhang M AD - State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Haihe Laboratory of Sustainable Chemical Transformations, Frontiers Science Center for New Organic Matter, Nankai University, Tianjin, China. FAU - Luo, Heng AU - Luo H AD - State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Haihe Laboratory of Sustainable Chemical Transformations, Frontiers Science Center for New Organic Matter, Nankai University, Tianjin, China. FAU - Chang, Yi AU - Chang Y AD - State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Haihe Laboratory of Sustainable Chemical Transformations, Frontiers Science Center for New Organic Matter, Nankai University, Tianjin, China. FAU - Hao, Sheng-Shi AU - Hao SS AD - State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Haihe Laboratory of Sustainable Chemical Transformations, Frontiers Science Center for New Organic Matter, Nankai University, Tianjin, China. FAU - Shi, Zhu-Jun AU - Shi ZJ AUID- ORCID: 0009-0004-2463-8030 AD - State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Haihe Laboratory of Sustainable Chemical Transformations, Frontiers Science Center for New Organic Matter, Nankai University, Tianjin, China. FAU - Xie, Jian-Hua AU - Xie JH AUID- ORCID: 0000-0003-3907-2530 AD - State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Haihe Laboratory of Sustainable Chemical Transformations, Frontiers Science Center for New Organic Matter, Nankai University, Tianjin, China. FAU - Wang, Xiao-Chen AU - Wang XC AUID- ORCID: 0000-0001-5863-0804 AD - State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Haihe Laboratory of Sustainable Chemical Transformations, Frontiers Science Center for New Organic Matter, Nankai University, Tianjin, China. LA - eng GR - 2021YFA1500200/National Key R&D Program of China/ GR - 22525104/National Natural Science Foundation of China/ GR - 22461160280/National Natural Science Foundation of China/ GR - 22371141/National Natural Science Foundation of China/ GR - 22221002/National Natural Science Foundation of China/ GR - 25ZXZSSS00700/Tianjin Major Science and Technology Project for National Key Laboratories/ GR - ZYTS202101/Haihe Laboratory of Sustainable Chemical Transformations/ GR - 63181206/Frontiers Science Center for New Organic Matter at Nankai University/ GR - Fundamental Research Funds for the Central Universities/ PT - Journal Article DEP - 20261029 PL - Germany TA - Angew Chem Int Ed Engl JT - Angewandte Chemie (International ed. in English) JID - 0370543 SB - IM OTO - NOTNLM OT - C horizontal line H functionalization OT - boron OT - fluorine OT - nitrogen heterocycles OT - radical reactions EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 02:53 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/25 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/30 02:53 [entrez] AID - e1059611 [pii] AID - 10.1002/anie.1059611 [doi] PST - aheadofprint SO - Angew Chem Int Ed Engl. 2026 Oct 29:e1059611. doi: 10.1002/anie.1059611. PMID- 42958829 OWN - NLM STAT- Publisher LR - 20261030 IS - 2287-285X (Electronic) IS - 2287-2728 (Linking) DP - 2026 Oct 29 TI - A step forward in predicting HCC recurrence after LT by integrating clinical factors and cell-free DNA-derived copy number alterations: Editorial on "Pre-operative risk assessment of HCC recurrence in liver transplant recipients by non-invasive detection of pre-existing genetic lesions". LID - 10.3350/cmh.2026.0325 [doi] FAU - Lee, Soon Kyu AU - Lee SK AD - Division of gastroenterology and hepatology, Department of Internal Medicine, Incheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea. blackiqq@gmail.com. LA - eng PT - Editorial DEP - 20261029 PL - Korea (South) TA - Clin Mol Hepatol JT - Clinical and molecular hepatology JID - 101586730 SB - IM OTO - NOTNLM OT - cell-free DNA OT - hepatocellular carcinoma OT - liver transplantation EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 02:45 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/03/16 00:00 [received] PHST- 2026/03/29 00:00 [accepted] PHST- 2026/10/30 02:45 [entrez] AID - cmh.2026.0325 [pii] AID - 10.3350/cmh.2026.0325 [doi] PST - aheadofprint SO - Clin Mol Hepatol. 2026 Oct 29. doi: 10.3350/cmh.2026.0325. PMID- 42958812 OWN - NLM STAT- Publisher LR - 20261030 IS - 1521-3773 (Electronic) IS - 1433-7851 (Linking) DP - 2026 Oct 29 TI - Spin Polarized Chiral Co(3)O(4) Boosts High-Pressure CO(2) Electroreduction to Ethanol With 90% Selectivity. LID - e1640517 [pii] LID - 10.1002/anie.1640517 [doi] AB - Electrocatalytic CO(2) reduction (CO(2)RR) to value-added multicarbon products using non-copper-based catalysts remains a major challenge, especially for selective ethanol production. Herein, we report that chiral Co(3)O(4) featuring strong electron spin polarization achieves a maximum ethanol Faradaic efficiency of 90% at -0.9 V versus NHE in KHCO(3) electrolytes under a 30 bar CO(2) pressure, substantially outperforming both racemic Co(3)O(4) and previously reported non-copper-based catalysts. The enhanced spin polarization in chiral Co(3)O(4) facilitates electron transfer and promotes the activation of the adsorbed CO(2), while also lowering the free energy change for C-C coupling to form *COCHO intermediate, thereby promoting ethanol production. Our work establishes spin polarization as a key determinant for efficient C-C coupling on non-copper-based catalysts and provides mechanistic principles for the rational design of CO(2)RR electrocatalyst. CI - (c) 2026 Wiley-VCH GmbH. FAU - Hu, Mingliang AU - Hu M AD - State Key Laboratory of Advanced Materials For Intelligent Sensing, Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, China. FAU - Sun, Mingzi AU - Sun M AUID- ORCID: 0000-0001-5136-7265 AD - Department of Chemistry, City University of Hong Kong, Kowloon, Hong Kong SAR, China. FAU - Zhao, Runyao AU - Zhao R AD - Beijing National Laboratory For Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China. FAU - Li, Junjun AU - Li J AD - State Key Laboratory of Advanced Materials For Intelligent Sensing, Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, China. AD - Department of Chemistry, Tsinghua University, Beijing, China. FAU - Ye, Yike AU - Ye Y AUID- ORCID: 0000-0001-6454-3483 AD - Nanyang Environment and Water Research Institute (NEWRI), Interdisciplinary Graduate School, Nanyang Technological University, 1 Cleantech Loop, Singapore, Singapore. FAU - Zhang, Yu AU - Zhang Y AD - State Key Laboratory of Advanced Materials For Intelligent Sensing, Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, China. FAU - Fu, Jiaju AU - Fu J AUID- ORCID: 0000-0002-0825-2566 AD - Beijing National Laboratory For Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China. FAU - Tang, Bing AU - Tang B AUID- ORCID: 0009-0002-4637-3809 AD - Department of Chemistry, Tsinghua University, Beijing, China. FAU - Yu, Can AU - Yu C AUID- ORCID: 0000-0002-1059-2180 AD - National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui, China. FAU - Ke, Yunzhe AU - Ke Y AD - State Key Laboratory of Advanced Materials For Intelligent Sensing, Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, China. FAU - Ding, Shuaishuai AU - Ding S AUID- ORCID: 0000-0003-1999-5127 AD - State Key Laboratory of Advanced Materials For Intelligent Sensing, Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, China. FAU - Ni, Bing AU - Ni B AUID- ORCID: 0000-0001-9657-6933 AD - College of Chemistry, Beijing Normal University, Beijing, China. FAU - Wang, Bo AU - Wang B AD - Tianjin Key Laboratory of Low Dimensional Materials Physics and Processing Technology, School of Science, Tianjin University, Tianjin, China. FAU - Jin, Chao AU - Jin C AUID- ORCID: 0000-0003-4731-7542 AD - Tianjin Key Laboratory of Low Dimensional Materials Physics and Processing Technology, School of Science, Tianjin University, Tianjin, China. FAU - Ye, Ruquan AU - Ye R AUID- ORCID: 0000-0002-2543-9090 AD - Department of Chemistry and State Key Laboratory of Marine Pollution, City University of Hong Kong, Hong Kong, China. FAU - Liu, Zhimin AU - Liu Z AUID- ORCID: 0000-0001-7953-7414 AD - Beijing National Laboratory For Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China. FAU - Xu, Zhichuan J AU - Xu ZJ AUID- ORCID: 0000-0001-7746-5920 AD - School of Materials Science and Engineering, Nanyang Technological University, Singapore, Singapore. FAU - Huang, Bolong AU - Huang B AUID- ORCID: 0000-0002-2526-2002 AD - Department of Chemistry, City University of Hong Kong, Kowloon, Hong Kong SAR, China. FAU - Zhang, Zhicheng AU - Zhang Z AUID- ORCID: 0000-0002-2487-4250 AD - State Key Laboratory of Advanced Materials For Intelligent Sensing, Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, China. FAU - Hu, Wenping AU - Hu W AUID- ORCID: 0000-0001-5686-2740 AD - State Key Laboratory of Advanced Materials For Intelligent Sensing, Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, China. LA - eng GR - 2023YFA1507904/National Key Research and Development Program of China/ GR - 2023YFA1507903/National Key Research and Development Program of China/ GR - U24B20190/National Natural Science Foundation of China/ GR - 22375142/National Natural Science Foundation of China/ GR - 2022DQ02-0408/Innovation Funding Project of Science and Technology, China National Petroleum Corporation/ GR - GZB20250243/Postdoctoral Fellowship Program of CPSF/ PT - Journal Article DEP - 20261029 PL - Germany TA - Angew Chem Int Ed Engl JT - Angewandte Chemie (International ed. in English) JID - 0370543 SB - IM OTO - NOTNLM OT - carbon dioxide electroreduction OT - chirality-induced spin polarization OT - ethanol electrosynthesis OT - high-pressure electrocatalysis OT - non‑Cu catalysts EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 02:43 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/10/12 00:00 [revised] PHST- 2026/08/26 00:00 [received] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/30 02:43 [entrez] AID - e1640517 [pii] AID - 10.1002/anie.1640517 [doi] PST - aheadofprint SO - Angew Chem Int Ed Engl. 2026 Oct 29:e1640517. doi: 10.1002/anie.1640517. PMID- 42958786 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 2287-9714 (Print) IS - 2287-9714 (Linking) VI - 42 IP - 5 DP - 2026 Oct TI - Comments on "Mini-invasive J-colpo-perineo-rectopexy with titanized polypropylene mesh for global perineal descent: a technical note". PG - 568-570 LID - 10.3393/ac.2026.00472.0067 [doi] FAU - Wadees, Alaa Abood Al AU - Wadees AAA AD - Department of General Surgery, Faculty of Medicine, Jabir Ibn Hayyan University for Medical and Pharmaceutical Sciences, Najaf, Iraq. LA - eng PT - Comment PT - Journal Article DEP - 20261015 PL - Korea (South) TA - Ann Coloproctol JT - Annals of coloproctology JID - 101605121 CON - Ann Coloproctol. 2026 Feb;42(1):141-144. doi: 10.3393/ac.2025.01214.0173. PMID: 41802314 EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 02:32 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/04/08 00:00 [received] PHST- 2026/08/19 00:00 [accepted] PHST- 2026/10/30 02:32 [entrez] AID - ac.2026.00472.0067 [pii] AID - 10.3393/ac.2026.00472.0067 [doi] PST - ppublish SO - Ann Coloproctol. 2026 Oct;42(5):568-570. doi: 10.3393/ac.2026.00472.0067. Epub 2026 Oct 15. PMID- 42958780 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 2287-9714 (Print) IS - 2287-9714 (Linking) VI - 42 IP - 5 DP - 2026 Oct TI - Oncological outcomes of robotic versus laparoscopic colectomy for colon cancer: a propensity score-matched study focusing on peritoneal recurrence. PG - 524-533 LID - 10.3393/ac.2026.00213.0030 [doi] AB - PURPOSE: To compare perioperative and oncologic outcomes between robotic and laparoscopic colectomy for colon cancer, with a focus on peritoneal recurrence, using a propensity score-matched analysis. METHODS: We retrospectively reviewed 591 patients who underwent minimally invasive colectomy for colon cancer between 2016 and 2024. After 1:1 propensity score matching based on age, sex, body mass index, American Society of Anesthesiologists physical status, clinical stage, and procedure, 90 patients in each group were analyzed. Survival outcomes were assessed using Kaplan-Meier methods and competing risk analysis with the Fine-Gray model. RESULTS: Robotic colectomy showed lower blood loss (median, 10 mL vs. 20 mL; P<0.001), shorter postoperative hospital stay (median, 9 days vs. 11.5 days, P<0.001), and a higher rate of intracorporeal anastomosis (92.2% vs. 24.4%, P<0.001), without prolonging operative time. Disease-free survival was comparable (P=0.519). Locoregional/peritoneal recurrence-free survival was higher in the robotic group (P=0.037); however, competing risk analysis using the Fine-Gray model showed no significant difference in the cumulative incidence of locoregional/peritoneal recurrence between groups. Multivariable analysis identified high-grade histology as an independent predictor of peritoneal recurrence, whereas surgical approach was not. CONCLUSION: Robotic colectomy provides favorable perioperative outcomes while maintaining oncologic safety comparable to laparoscopic colectomy. Peritoneal recurrence appears to be driven primarily by tumor biology, and intracorporeal anastomosis does not increase recurrence risk. FAU - Nonaka, Takashi AU - Nonaka T AD - Division of Surgical Oncology, Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. AD - Division of Colorectal Surgery, Department of Surgery, Nagasaki University Hospital, Nagasaki, Japan. AD - Minimally Invasive Surgery Center, Nagasaki University Hospital, Nagasaki, Japan. FAU - Noda, Keisuke AU - Noda K AD - Division of Surgical Oncology, Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. AD - Division of Colorectal Surgery, Department of Surgery, Nagasaki University Hospital, Nagasaki, Japan. FAU - Tominaga, Tetsuro AU - Tominaga T AD - Division of Surgical Oncology, Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. AD - Division of Colorectal Surgery, Department of Surgery, Nagasaki University Hospital, Nagasaki, Japan. FAU - Katayama, Hiroki AU - Katayama H AD - Division of Surgical Oncology, Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. AD - Division of Colorectal Surgery, Department of Surgery, Nagasaki University Hospital, Nagasaki, Japan. FAU - Hashimoto, Shintaro AU - Hashimoto S AD - Division of Surgical Oncology, Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. AD - Division of Colorectal Surgery, Department of Surgery, Nagasaki University Hospital, Nagasaki, Japan. FAU - Hisanaga, Makoto AU - Hisanaga M AD - Department of Surgery, Sasebo City General Hospital, Nagasaki, Japan. FAU - Takeshita, Hiroaki AU - Takeshita H AD - Department of Surgery, National Hospital Organization Nagasaki Medical Center, Nagasaki, Japan. FAU - To, Kazuo AU - To K AD - Department of Surgery, Ureshino Medical Center, Ureshino, Japan. FAU - Fukuoka, Hidetoshi AU - Fukuoka H AD - Department of Surgery, Isahaya General Hospital, Nagasaki, Japan. FAU - Matsumoto, Keitaro AU - Matsumoto K AD - Division of Surgical Oncology, Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. LA - eng PT - Journal Article DEP - 20261001 PL - Korea (South) TA - Ann Coloproctol JT - Annals of coloproctology JID - 101605121 OTO - NOTNLM OT - Local neoplasm recurrence OT - Robotic surgical procedures OT - Surgical anastomosis EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 02:32 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/01/31 00:00 [received] PHST- 2026/05/20 00:00 [accepted] PHST- 2026/10/30 02:32 [entrez] AID - ac.2026.00213.0030 [pii] AID - 10.3393/ac.2026.00213.0030 [doi] PST - ppublish SO - Ann Coloproctol. 2026 Oct;42(5):524-533. doi: 10.3393/ac.2026.00213.0030. Epub 2026 Oct 1. PMID- 42958777 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 2287-9714 (Print) IS - 2287-9714 (Linking) VI - 42 IP - 5 DP - 2026 Oct TI - Clinicopathologic characteristics and molecular landscape for pseudomyxoma peritonei of appendiceal origin. PG - 492-504 LID - 10.3393/ac.2026.00031.0004 [doi] AB - Pseudomyxoma peritonei (PMP) of appendiceal origin presents a unique challenge in oncology owing to its rarity, varied progression, and complex molecular characteristics. The current standard treatment using cytoreductive surgery combined with hyperthermic intraperitoneal chemotherapy has shown some success in extending survival rates; however, it is associated with significant physical burdens and high recurrence rates. Advances in understanding the molecular and genetic features, particularly mutations in genes such as KRAS, GNAS, and BRAF, have led to a promising shift toward more targeted and less invasive treatment options, potentially moving beyond the limitations of current therapies. Additionally, novel approaches targeting the tumor microenvironment in PMP have gained traction, given the role of factors such as mucin accumulation, hypoxia, and inflammatory cytokines in sustaining tumor persistence and resistance. Emerging personalized therapies and immunotherapies, particularly targeted treatments and peptide vaccine development for GNAS-mutated cancers, offer the potential to enhance treatment specificity and effectiveness. The challenges of multiomics approaches to capture the complex genomic and epigenomic landscape of PMP highlight the need for individualized optimal treatment in patients with PMP. FAU - Yoon, Taehan AU - Yoon T AD - Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea. FAU - Shin, Su-Jin AU - Shin SJ AD - Department of Pathology, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Korea. FAU - Lee, Ji Min AU - Lee JM AD - Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea. FAU - Park, Eun Jung AU - Park EJ AD - Division of Colon and Rectal Surgery, Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. LA - eng GR - RS-2022-NR069827/Ministry of Science and ICT/ GR - RS-2024-00407383/Ministry of Science and ICT/ GR - National Research Foundation of Korea/ GR - 2024IF0030/Department of Surgery, Asan Medical Center/ GR - 2025IP0041/Asan Institute for Life Sciences, Asan Medical Center/ GR - 2025IP0028/Asan Institute for Life Sciences, Asan Medical Center/ PT - Journal Article PT - Review DEP - 20261015 PL - Korea (South) TA - Ann Coloproctol JT - Annals of coloproctology JID - 101605121 OTO - NOTNLM OT - Appendiceal neoplasms OT - Hypoxia OT - Mucins OT - Pseudomyxoma peritonei OT - Tumor microenvironment EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 02:32 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/01/02 00:00 [received] PHST- 2026/05/09 00:00 [accepted] PHST- 2026/10/30 02:32 [entrez] AID - ac.2026.00031.0004 [pii] AID - 10.3393/ac.2026.00031.0004 [doi] PST - ppublish SO - Ann Coloproctol. 2026 Oct;42(5):492-504. doi: 10.3393/ac.2026.00031.0004. Epub 2026 Oct 15. PMID- 42958763 OWN - NLM STAT- Publisher LR - 20261030 IS - 2198-3844 (Electronic) IS - 2198-3844 (Linking) DP - 2026 Oct 29 TI - A Damage-Gated Small-Molecule Chimera Selectively Enhances Mitophagy by Coupling Dysfunctional Mitochondria to Autophagy. LID - e78570 [pii] LID - 10.1002/advs.78570 [doi] AB - Mitophagy is a central mitochondrial quality-control pathway, and its pharmacological activation is considered to be geroprotective. However, its small-molecule activators act indirectly by perturbing metabolism or mitochondrial function, limiting selectivity and increasing toxicity. Here, we describe a chemical biology strategy for activating damage-responsive mitophagy using bifunctional small molecules that couple mitochondrial recognition to the recruitment of the autophagic machinery. We designed and synthesized a focused library of chimeric molecules and identified MG-1 as a lead compound. It had minimal effects on basal autophagy but markedly enhanced autophagic flux and mitochondrial clearance under conditions of mitochondrial stress. Biochemical analyses, together with fluorescence and electron microscopy, showed that MG-1 promotes lysosomal degradation of damaged mitochondria without acting as a direct mitochondrial uncoupler. MG-1 was well tolerated across experimental systems and improved locomotor performance in a zebrafish model of MPTP-induced mitochondrial dysfunction. These findings establish damage-gated small-molecule chimeras as a promising approach for selective control of mitochondrial quality. CI - (c) 2026 The Author(s). Advanced Science published by Wiley-VCH GmbH. FAU - Ozhiganov, Ratislav M AU - Ozhiganov RM AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia. AD - Chemical Department, Lomonosov Moscow State University, Moscow, Russia. FAU - Guseva, Ekaterina capital A, Cyrillic AU - Guseva Ecapital A, Cyrillic AUID- ORCID: 0000-0002-1818-6590 AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia. AD - Chemical Department, Lomonosov Moscow State University, Moscow, Russia. FAU - Rudenko, Alexander Yu AU - Rudenko AY AUID- ORCID: 0000-0002-8320-0539 AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia. AD - Chemical Department, Lomonosov Moscow State University, Moscow, Russia. FAU - Lyamzaev, Konstantin G AU - Lyamzaev KG AUID- ORCID: 0000-0001-9921-8031 AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia. FAU - Sokolskaya, Sofya Y AU - Sokolskaya SY AD - Faculty of Fundamental Medicine, M.V. Lomonosov Moscow State University, Moscow, Russia. FAU - Golubeva, Julia A AU - Golubeva JA AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia. AD - Chemical Department, Lomonosov Moscow State University, Moscow, Russia. FAU - Belopolskaya, Maria V AU - Belopolskaya MV AUID- ORCID: 0000-0002-6772-2580 AD - Institute of Mitoengineering, Lomonosov Moscow State University, Moscow, Russia. FAU - Andrianova, Nadezda V AU - Andrianova NV AUID- ORCID: 0000-0003-2720-4575 AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia. FAU - Fadeeva, Olga V AU - Fadeeva OV AUID- ORCID: 0000-0001-6833-8313 AD - Institute of Mitoengineering, Lomonosov Moscow State University, Moscow, Russia. FAU - Morgun, Elena capital O, Cyrillic AU - Morgun Ecapital O, Cyrillic AD - Pirogov Russian National Research Medical University, Moscow, Russia. FAU - Tukhovskaya, Elena A AU - Tukhovskaya EA AUID- ORCID: 0000-0001-6174-4052 AD - Shemyakin-Ovchinnicov Institute of Bioorganic Chemistry (branch), Russian Academy of Sciences, Pushchino, Russia. FAU - Kazakova, Marina S AU - Kazakova MS AD - Shemyakin-Ovchinnicov Institute of Bioorganic Chemistry (branch), Russian Academy of Sciences, Pushchino, Russia. FAU - Pavlov, Vladimir M AU - Pavlov VM AUID- ORCID: 0009-0003-8949-5600 AD - Shemyakin-Ovchinnicov Institute of Bioorganic Chemistry (branch), Russian Academy of Sciences, Pushchino, Russia. FAU - Yagoda, Daria K AU - Yagoda DK AD - Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia. FAU - Marusich, Elena I AU - Marusich EI AD - Moscow Center for Advanced Studies, Moscow, Russia. FAU - Zaranaina, Rose-Lys AU - Zaranaina RL AUID- ORCID: 0009-0000-3444-7351 AD - Peoples' Friendship University of Russia named After Patrice Lumumba, Moscow, Russia. FAU - Vays, Valeriya B AU - Vays VB AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia. FAU - Vangeli, Irina M AU - Vangeli IM AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia. FAU - Plotnikov, Egor Y AU - Plotnikov EY AUID- ORCID: 0000-0003-2838-3704 AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia. FAU - Lovat, Maxim L AU - Lovat ML AD - Institute of Mitoengineering, Lomonosov Moscow State University, Moscow, Russia. AD - Biological Faculty, Lomonosov Moscow State University, Moscow, Russia. FAU - Bakeeva, Lora E AU - Bakeeva LE AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia. FAU - Murashev, Arkady N AU - Murashev AN AD - Shemyakin-Ovchinnicov Institute of Bioorganic Chemistry (branch), Russian Academy of Sciences, Pushchino, Russia. FAU - Polshakov, Vladimir I AU - Polshakov VI AUID- ORCID: 0000-0002-3216-5737 AD - Chemical Department, Lomonosov Moscow State University, Moscow, Russia. FAU - Dontsova, Olga A AU - Dontsova OA AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia. AD - Chemical Department, Lomonosov Moscow State University, Moscow, Russia. AD - Shemyakin-Ovchinnicov Institute of Bioorganic Chemistry (branch), Russian Academy of Sciences, Pushchino, Russia. FAU - Sergiev, Peter V AU - Sergiev PV AUID- ORCID: 0000-0001-8866-1863 AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia. AD - Chemical Department, Lomonosov Moscow State University, Moscow, Russia. LA - eng GR - numero sign 25-74-31004/Russian Science Foundation/ PT - Journal Article DEP - 20261029 PL - Germany TA - Adv Sci (Weinh) JT - Advanced science (Weinheim, Baden-Wurttemberg, Germany) JID - 101664569 SB - IM OTO - NOTNLM OT - autophagy OT - bifunctional molecules OT - damage-responsive autophagy OT - drug discovery OT - medicinal chemistry OT - mitophagy OT - neurodegeneration OT - small-molecule chimeras EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 02:23 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/10/19 00:00 [revised] PHST- 2026/04/28 00:00 [received] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/30 02:23 [entrez] AID - e78570 [pii] AID - 10.1002/advs.78570 [doi] PST - aheadofprint SO - Adv Sci (Weinh). 2026 Oct 29:e78570. doi: 10.1002/advs.78570. PMID- 42958759 OWN - NLM STAT- Publisher LR - 20261030 IS - 2198-3844 (Electronic) IS - 2198-3844 (Linking) DP - 2026 Oct 29 TI - An Epidermal Sweat Patch for Tracking Metabolite Levels and Correlation Analysis in Biofluids. LID - e78544 [pii] LID - 10.1002/advs.78544 [doi] AB - Continuous, in situ monitoring of sweat biomarkers offers a promising approach for non-invasive metabolic assessment, but its practical implementation remains limited by sensing stability and the insufficiently defined physiological relationship between sweat and systemic biofluids. Here, we develop an epidermal sweat patch that integrates a non-enzymatic electrochemical interface based on Au-Ni(OH)(2) dual aerogels for glucose monitoring. The complementary roles of Ni(OH)(2)-mediated glucose adsorption and Au-assisted electrooxidation enable sensitive and stable sensing, with a calculated detection limit of 0.1 microM and >95% signal retention after 5 weeks. Combined with microfluidic sweat sampling, signal processing and wireless communication, the patch enables real-time on-body tracking of sweat glucose during daily activities. In human studies, sweat glucose shows positive associations with interstitial fluid and blood glucose, with Pearson correlation coefficients of up to 0.828, while subgroup analyses further reveal interindividual variability. These results support the physiological relevance of sweat glucose as a complementary signal for metabolic monitoring. Overall, this work integrates non-enzymatic sensing, wearable system design and cross-biofluid analysis within a single platform, providing a practical framework for physiologically interpretable sweat-based glucose monitoring. CI - (c) 2026 The Author(s). Advanced Science published by Wiley-VCH GmbH. FAU - Chen, Yao AU - Chen Y AUID- ORCID: 0009-0007-3772-2866 AD - Department of Hepatobiliary Surgery and Liver Transplantation, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of China. AD - State Key Laboratory of Solidification Processing, School of Materials Science and Engineering, Northwestern Polytechnical University, Xi'an, People's Republic of China. FAU - Wan, Xinhao AU - Wan X AUID- ORCID: 0009-0008-7720-9917 AD - State Key Laboratory of Solidification Processing, School of Materials Science and Engineering, Northwestern Polytechnical University, Xi'an, People's Republic of China. FAU - Fan, Haoxin AU - Fan H AUID- ORCID: 0009-0000-1139-5827 AD - State Key Laboratory of Solidification Processing, School of Materials Science and Engineering, Northwestern Polytechnical University, Xi'an, People's Republic of China. FAU - Lou, Hengwei AU - Lou H AUID- ORCID: 0009-0003-8518-5007 AD - State Key Laboratory of Solidification Processing, School of Materials Science and Engineering, Northwestern Polytechnical University, Xi'an, People's Republic of China. FAU - Wang, Jialu AU - Wang J AUID- ORCID: 0009-0000-8534-335X AD - State Key Laboratory of Solidification Processing, School of Materials Science and Engineering, Northwestern Polytechnical University, Xi'an, People's Republic of China. FAU - Gao, Jie AU - Gao J AUID- ORCID: 0000-0003-1405-511X AD - School of Life Sciences, Northwestern Polytechnical University, Xi'an, People's Republic of China. FAU - Wen, Dan AU - Wen D AUID- ORCID: 0000-0001-6879-7982 AD - State Key Laboratory of Solidification Processing, School of Materials Science and Engineering, Northwestern Polytechnical University, Xi'an, People's Republic of China. LA - eng GR - 22374119/National Natural Science Foundation of China/ GR - 22504112/National Natural Science Foundation of China/ GR - JYB2025XDXM406/Fundamental and Interdisciplinary Disciplines Breakthrough Plan of the Ministry of Education of China/ GR - 2023-JC-ZD-06/Key Project of Natural Science Fund of Shaanxi Province/ GR - 2025M781013/China Postdoctoral Science Foundation/ PT - Journal Article DEP - 20261029 PL - Germany TA - Adv Sci (Weinh) JT - Advanced science (Weinheim, Baden-Wurttemberg, Germany) JID - 101664569 SB - IM OTO - NOTNLM OT - biofluids OT - correlation analysis OT - glucose monitoring OT - metal aerogels OT - wearable biosensors EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 02:23 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/08 00:00 [revised] PHST- 2026/08/13 00:00 [received] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/30 02:23 [entrez] AID - e78544 [pii] AID - 10.1002/advs.78544 [doi] PST - aheadofprint SO - Adv Sci (Weinh). 2026 Oct 29:e78544. doi: 10.1002/advs.78544. PMID- 42958750 OWN - NLM STAT- Publisher LR - 20261030 IS - 1526-4998 (Electronic) IS - 1526-498X (Linking) DP - 2026 Oct 29 TI - The ecological adaptability of pinewood nematode populations: survival strategies, mechanisms and co-evolution. LID - 10.1002/ps.71433 [doi] AB - BACKGROUND: Bursaphelenchus xylophilus (pinewood nematode, PWN) is the causal agent of pine wilt disease and ranks among the most destructive invasive pests threatening global forest ecosystems. Since its introduction to China, this nematode has triggered massive ecological and economic losses, while its distribution range continues to expand northward. The association between the widespread dispersal of B. xylophilus in invaded areas and the ecological adaptability of its populations has drawn considerable attention. RESULTS: This review systematically synthesizes recent research advances regarding the ecological adaptability of PWN populations, elaborating on molecular mechanisms and ecological strategies that enable the nematode to colonize novel environments. In terms of thermal adaptation, differentially expressed genes, small heat shock protein families and the accumulation of key metabolites collectively shape adaptive pathways to resist extreme high and low temperature stress. For host adaptation, PWN displays polyphagous feeding habits and relies on diverse arboreal fungi as nutritional resources when pines were weakened or died. Detoxification enzyme cascades and effector proteins enable the nematode to counteract host defensive metabolites. Specifically, effectors suppress host peroxidase activity, thereby quenching reactive oxygen species bursts and disrupting plant hormone regulatory pathways to attenuate pine immune responses. Co-evolutionary interactions between PWN and vector beetles manifest across multiple dimensions, including synchronized life cycles, specialized dispersal-stage juveniles, and infochemical-mediated interspecies communication. PWN also forms intricate interaction networks with associated fungi and bacteria, which exert vital functions in nutrient acquisition, pathogenicity enhancement and terpenoid degradation. Notably, ascaroside pheromones serve as central signaling molecules that modulate interspecific competition and reproductive phenotypic plasticity. Population genetic analyses have uncovered shifts in genetic diversity, regulatory pathways governing population structure and reproductive efficiency, as well as synergistic evolutionary correlations between invasive population genetics and pathogenicity. Multi-omics analytical frameworks provide novel perspectives for dissecting the adaptive evolution of PWN. CONCLUSION: The invasive success and widespread dispersal of B. xylophilus are fundamentally driven by its strong population ecological adaptability. Finally, this review summarizes the existing research gaps and proposes future research priorities alongside targeted pest management strategies. (c) 2026 Society of Chemical Industry. CI - (c) 2026 Society of Chemical Industry. FAU - Ying, Chen-Xi AU - Ying CX AD - Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China. AD - College of Forestry, Nanjing Forestry University, Nanjing, China. FAU - Wen, Tong-Yue AU - Wen TY AUID- ORCID: 0000-0002-1889-1388 AD - Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China. AD - College of Forestry, Nanjing Forestry University, Nanjing, China. FAU - Ding, Xiao-Lei AU - Ding XL AD - Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China. AD - College of Forestry, Nanjing Forestry University, Nanjing, China. FAU - Wu, Xiao-Qin AU - Wu XQ AD - Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China. AD - College of Forestry, Nanjing Forestry University, Nanjing, China. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - Pest Manag Sci JT - Pest management science JID - 100898744 SB - IM OTO - NOTNLM OT - associated microorganisms OT - host adaptation OT - pinewood nematode OT - population genetics OT - temperature stress OT - vector insects EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 02:13 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/09/28 00:00 [revised] PHST- 2026/04/19 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/30 02:13 [entrez] AID - 10.1002/ps.71433 [doi] PST - aheadofprint SO - Pest Manag Sci. 2026 Oct 29. doi: 10.1002/ps.71433. PMID- 42958748 OWN - NLM STAT- Publisher LR - 20261030 IS - 2198-3844 (Electronic) IS - 2198-3844 (Linking) DP - 2026 Oct 29 TI - A UBE3A-UTX Proteostasis-Epigenetic Axis Governs Hypothalamic Energy Homeostasis. LID - e78567 [pii] LID - 10.1002/advs.78567 [doi] AB - Energy homeostasis requires integration of metabolic and stress signals within hypothalamic circuits, yet the role of neuronal proteostasis remains unclear. Ubiquitin protein ligase E3A (UBE3A) regulates the stability of lysine demethylase 6A (KDM6A), also known as ubiquitously transcribed tetratricopeptide repeat containing, X chromosome (UTX). Hypothalamic neuronal deletion of Ube3a results in hyperphagia, obesity, hyperleptinemia, and impaired glucose homeostasis under high-fat diet conditions; selective deletion in agouti-related peptide (AgRP) neurons is sufficient to recapitulate these phenotypes. UBE3A promotes UTX degradation, whereas UBE3A loss stabilizes UTX and decreases repressive histone H3 lysine 27 trimethylation (H3K27me3). Integrated transcriptomic and epigenomic analyses implicate UTX and activating transcription factor 4 (ATF4) in altered endoplasmic reticulum (ER) stress-responsive transcriptional programs, although their causal contribution to the metabolic phenotype remains to be established. Treatment with GSK-J4, an inhibitor of KDM6-family demethylases, increases hypothalamic H3K27me3 levels and improves metabolic outcomes in Ube3a-deficient mice, although the specific contribution of hypothalamic UTX inhibition to these effects remains to be established. These findings support a link between neuronal proteostasis, chromatin regulation, and systemic metabolic homeostasis and warrant further investigation of the relevance of UTX-associated pathways to metabolic dysfunction in a subset of individuals with Angelman syndrome. CI - (c) 2026 The Author(s). Advanced Science published by Wiley-VCH GmbH. FAU - Lee, Junekyoung AU - Lee J AUID- ORCID: 0009-0003-4985-3696 AD - College of Pharmacy, Seoul National University, Seoul, Republic of Korea. FAU - Yoo, Jaeyoung AU - Yoo J AUID- ORCID: 0009-0002-5740-5327 AD - College of Pharmacy, Seoul National University, Seoul, Republic of Korea. FAU - Kim, Young A AU - Kim YA AD - College of Pharmacy, Seoul National University, Seoul, Republic of Korea. FAU - Wei, Zhuodu AU - Wei Z AUID- ORCID: 0000-0003-4344-6427 AD - College of Pharmacy, Seoul National University, Seoul, Republic of Korea. FAU - Lee, Yunseo AU - Lee Y AD - College of Pharmacy, Seoul National University, Seoul, Republic of Korea. FAU - Shon, Woo-Jeong AU - Shon WJ AUID- ORCID: 0000-0002-5521-5768 AD - Department of Biopharmaceutical Sciences, Cheongju University, Cheongju, Republic of Korea. FAU - Seong, Je Kyung AU - Seong JK AD - Korea Mouse Phenotyping Center, Seoul National University, Seoul, Republic of Korea. FAU - Lee, Seunghee AU - Lee S AUID- ORCID: 0000-0001-5483-0016 AD - College of Pharmacy, Seoul National University, Seoul, Republic of Korea. AD - Research Institute of Pharmaceutical Sciences and Natural Products Research Institute, Seoul National University, Seoul, Republic of Korea. AD - Seoul National University Energy Initiative, Seoul National University, Seoul, Republic of Korea. LA - eng GR - RS-2024-00453874/National Research Foundation of Korea/ GR - 2013M3A9D5072550/Korea Mouse Phenotyping Project/ GR - Ministry of Science, ICT and Future Planning/ PT - Journal Article DEP - 20261029 PL - Germany TA - Adv Sci (Weinh) JT - Advanced science (Weinheim, Baden-Wurttemberg, Germany) JID - 101664569 SB - IM OTO - NOTNLM OT - ER stress OT - UBE3A OT - UTX OT - hypothalamic neurons OT - obesity EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 02:13 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/16 00:00 [revised] PHST- 2026/05/28 00:00 [received] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/30 02:13 [entrez] AID - e78567 [pii] AID - 10.1002/advs.78567 [doi] PST - aheadofprint SO - Adv Sci (Weinh). 2026 Oct 29:e78567. doi: 10.1002/advs.78567. PMID- 42958737 OWN - NLM STAT- Publisher LR - 20261030 IS - 1097-0010 (Electronic) IS - 0022-5142 (Linking) DP - 2026 Oct 29 TI - Chemical composition and antimicrobial potential of part-specific essential oils from Eucalyptus tereticornis: insights from molecular docking and network pharmacology. LID - 10.1002/jsfa.71189 [doi] AB - BACKGROUND: Growing concerns over synthetic antimicrobials have increased interest in plant-derived essential oils as safer alternatives. Eucalyptus tereticornis Sm. (Myrtaceae) is known for its bioactivity; however, variations in chemical composition and antimicrobial potential among plant parts remain poorly understood. RESULTS: Essential oils (EOs) were extracted from leaves, fruits, and stems and characterized by gas chromatography-mass spectrometry (GC-MS), revealing 24 phytoconstituents with distinct part-specific profiles. Leaf EO was dominated by alpha-phellandrene (49.26%). Fruit EO exhibited stronger antibacterial activity against gram-positive than gram-negative strains, with Micrococcus luteus showing the largest inhibition zone (27.86 +/- 0.18 mm) and a minimum inhibitory concentration (MIC) of 2.5 mg mL(-1). Stem EO demonstrated good antibiofilm activity, disrupting 64.08% of the biofilm produced by M. luteus. Among the tested fungi, Fusarium graminearum showed the highest susceptibility to stem EO, with an inhibition zone of 22.43 +/- 0.24 mm, an MIC of 1.25 mg mL(-1), and a minimum fungicidal concentration (MFC) of 5 mg mL(-1) (MFC/MIC ratio = 4), confirming its fungicidal effect. Molecular docking identified alpha-eudesmol as the strongest binder to the fungal target (-8.29 kcal mol(-1)), exceeding fluconazole (-7.64 kcal mol(-1)) and carbendazim (-7.10 kcal mol(-1)). Network pharmacology suggested the involvement of the PI3K-Akt, IL-17, FoxO, and Th17 signaling pathways. Absorption, distribution, metabolism, excretion, and toxicity (ADMET) analysis predicted favorable physicochemical properties for most constituents. CONCLUSION: Part-specific E. tereticornis essential oils exhibit selective antimicrobial effects with potential applications in food preservation and sustainable crop protection. Molecular docking, network pharmacology, and ADMET analyses provided complementary mechanistic insights; however, these computational predictions require further experimental and in vivo validation. (c) 2026 Society of Chemical Industry. CI - (c) 2026 Society of Chemical Industry. FAU - Sharma, Ritesh AU - Sharma R AUID- ORCID: 0009-0009-6900-7802 AD - Chemical Technology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, India. AD - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India. FAU - Sharma, Pallavi AU - Sharma P AD - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India. AD - Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, India. FAU - Dev, Kapil AU - Dev K AD - Chemical Technology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, India. AD - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India. FAU - Sharma, Sanjeev Kumar AU - Sharma SK AD - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India. AD - Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, India. FAU - Pati, Aparna Maitra AU - Pati AM AD - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India. AD - Biotechnology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, India. FAU - Agnihotri, Vijai K AU - Agnihotri VK AD - Chemical Technology Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, India. AD - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India. LA - eng GR - National Laboratories Scheme under the ULIP sub scheme (MLP-002619) and CRISP sub-scheme (HCP-0007)/ PT - Journal Article DEP - 20261029 PL - England TA - J Sci Food Agric JT - Journal of the science of food and agriculture JID - 0376334 SB - IM OTO - NOTNLM OT - Eucalyptus tereticornis OT - antimicrobial OT - essential oil OT - gas chromatography OT - molecular docking OT - network pharmacology EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 02:04 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/08/15 00:00 [revised] PHST- 2026/02/10 00:00 [received] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/30 02:04 [entrez] AID - 10.1002/jsfa.71189 [doi] PST - aheadofprint SO - J Sci Food Agric. 2026 Oct 29. doi: 10.1002/jsfa.71189. PMID- 42958709 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1868-1751 (Electronic) IS - 1868-1743 (Linking) VI - 45 IP - 11 DP - 2026 Nov TI - Interpretable Drug-Target Prediction via Geometric Alignment: A Linear Alternative to Deep Architectural Complexity. LID - e70060 [pii] LID - 10.1002/minf.70060 [doi] AB - Designing selective kinase inhibitors is a multiobjective optimization problem constrained by the structural conservation of the human kinome, along with the lack of high-quality interaction data. Traditional deep learning methods often train complex neural networks directly on small datasets and fail to generalize to new chemical scaffolds. To this end, we present Geometric Manifold Alignment, a novel linear approach that shifts away from architectural complexity. We introduce Multi-View Linear Manifold (MVLM), which aligns the latent spaces of ChemBERTa (small molecules) and ESM-2 (protein sequences) into a shared geometric interaction space. We pretrain MVLM on the Universal Foundation Set of 473 923 high-confidence interactions, where binding affinity is represented as geometric proximity between drug and target embeddings. Achieving an AUROC of 0.936 on KIBA and 0.928 on Davis, MVLM establishes the new state-of-the-art on industry-standard benchmarks, outperforming existing methods (p < 0.001). In this work, interpretability is geometric where interaction scores are cosine similarities in a shared manifold, and the learned structure recovers kinase phylogenetic relationships without supervision. We also demonstrate the model's utility as a transparent discovery engine by retrieving known clinical targets of FDA-approved drugs. Our findings suggest that aligning pretrained chemical and biological representations provides a scalable and interpretable approach to advancing computational drug discovery. CI - (c) 2026 The Author(s). Molecular Informatics published by Wiley-VCH GmbH. FAU - Ghimire, Aashutosh AU - Ghimire A AUID- ORCID: 0000-0003-1864-9636 AD - Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana, USA. FAU - Alvin, Chris AU - Alvin C AUID- ORCID: 0000-0001-6044-2159 AD - Department of Computer Science, Furman University, Greenville, South Carolina, USA. FAU - Bess, Adam AU - Bess A AUID- ORCID: 0000-0003-1156-076X AD - Department of Computer Science, Louisiana State University, Baton Rouge, Louisiana, USA. FAU - Mukhopadhyay, Supratik AU - Mukhopadhyay S AUID- ORCID: 0000-0003-0839-1133 AD - Department of Environmental Sciences, Louisiana State University, Baton Rouge, Louisiana, USA. LA - eng PT - Journal Article PL - Germany TA - Mol Inform JT - Molecular informatics JID - 101529315 RN - 0 (Protein Kinase Inhibitors) RN - EC 2.7.- (Protein Kinases) SB - IM MH - *Protein Kinase Inhibitors/chemistry/pharmacology MH - Humans MH - *Deep Learning MH - *Drug Discovery/methods MH - Protein Kinases/chemistry OTO - NOTNLM OT - computational drug discovery OT - contrastive learning OT - drug-target interaction OT - foundation models OT - interpretable machine learning OT - kinase inhibitor selectivity OT - latent space alignment EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:43 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/09/01 00:00 [revised] PHST- 2026/06/29 00:00 [received] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/30 01:43 [entrez] AID - e70060 [pii] AID - 10.1002/minf.70060 [doi] PST - ppublish SO - Mol Inform. 2026 Nov;45(11):e70060. doi: 10.1002/minf.70060. PMID- 42958694 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1742-6723 (Electronic) IS - 1742-6723 (Linking) VI - 38 IP - 6 DP - 2026 Dec TI - Vaping and E-Cigarettes: An Emergency Department Survey. LID - e70367 [pii] LID - 10.1111/1742-6723.70367 [doi] AB - OBJECTIVE: To characterise vaping practices and perceptions in a non-consecutive cohort across four Sydney Emergency Departments (EDs) prior to 2024 Australian legislative changes restricting disposable vapes. METHODS: An anonymous digital survey of up to 40 questions was conducted from May 2023 to January 2024 in patients and visitors aged > 14 years. It assessed demographics, vaping and smoking practices, perceived addiction, side effects and attitudes. Participants were recruited via posters and distribution of linked QR codes. RESULTS: Of 1263 surveys initiated, 1049 (83.1%) were completed. Among respondents who answered the vaping-status question 51.1% (614/1202) reported ever vaping with 34.4% (211/614) vaping daily. Respondents were more often able to report the puff count and duration of their most recently purchased device (> 85%) than puffs per day or sessions per day ( approximately 60%). Disposable vapes and fruit/candy flavours were most reportedly used. Addiction and anxiety relief were frequently selected reasons for vaping, and daily vapers reported higher perceived addiction scores. Symptoms occurring during or shortly after vaping were commonly reported, most frequently cough (174/308, 56.5%), breathlessness (165/308, 53.6%) and chest pain (98/308, 31.8%). Daily vapers more commonly perceived vaping as safer than cigarettes (101/198, 51.0% vs. 88/568, 15.5% in never vapers) and as an effective smoking cessation aid (105/197, 53.3% vs. 83/571, 14.5%). CONCLUSION: In this multicentre, non-consecutive survey, vaping was commonly reported, particularly among younger respondents, with frequent daily use and perceived addiction. These findings highlight the importance of asking about vaping and awareness of patterns of use, perceived addiction and reported symptoms. CI - (c) 2026 The Author(s). Emergency Medicine Australasia published by John Wiley & Sons Australia, Ltd on behalf of Australasian College for Emergency Medicine. FAU - Lycett, Christopher AU - Lycett C AUID- ORCID: 0009-0004-7131-141X AD - Department of Emergency Medicine, Prince of Wales Hospital, Sydney, New South Wales, Australia. AD - Department of Emergency Medicine, St George Hospital, Sydney, New South Wales, Australia. AD - University of New South Wales, Sydney, New South Wales, Australia. FAU - Jagarlamudi, Bhashita AU - Jagarlamudi B AD - Department of Emergency Medicine, Prince of Wales Hospital, Sydney, New South Wales, Australia. AD - Department of Emergency Medicine, St Vincents Hospital, Sydney, New South Wales, Australia. FAU - Moore, Allison AU - Moore A AUID- ORCID: 0009-0009-7407-6044 AD - Department of Emergency Medicine, Sutherland Hospital, Sydney, New South Wales, Australia. FAU - Minhas, Sanjeet AU - Minhas S AUID- ORCID: 0009-0000-4908-7125 AD - Department of Emergency Medicine, St George Hospital, Sydney, New South Wales, Australia. FAU - Briggs, Nancy AU - Briggs N AUID- ORCID: 0000-0002-1134-0807 AD - University of New South Wales, Sydney, New South Wales, Australia. FAU - Chiew, Angela AU - Chiew A AUID- ORCID: 0000-0002-0079-5056 AD - Department of Emergency Medicine, Prince of Wales Hospital, Sydney, New South Wales, Australia. AD - University of New South Wales, Sydney, New South Wales, Australia. LA - eng PT - Journal Article PL - Australia TA - Emerg Med Australas JT - Emergency medicine Australasia : EMA JID - 101199824 SB - IM MH - Humans MH - *Vaping/epidemiology/psychology/adverse effects MH - Female MH - Emergency Service, Hospital/organization & administration/statistics & numerical data MH - Adult MH - *Electronic Nicotine Delivery Systems/statistics & numerical data MH - Surveys and Questionnaires MH - Male MH - Middle Aged MH - Australia/epidemiology MH - Adolescent OTO - NOTNLM OT - emergency department OT - e-cigarette OT - perceptions OT - vape OT - vaping EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:33 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/09/10 00:00 [revised] PHST- 2026/05/06 00:00 [received] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/30 01:33 [entrez] AID - e70367 [pii] AID - 10.1111/1742-6723.70367 [doi] PST - ppublish SO - Emerg Med Australas. 2026 Dec;38(6):e70367. doi: 10.1111/1742-6723.70367. PMID- 42958681 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2589-451X (Electronic) IS - 0255-2922 (Linking) VI - 46 IP - 5 DP - 2026 Oct TI - Pharmacological advances of alkaloids from Traditional Chinese Medicine against pulmonary fibrosis: focus on anti-inflammatory mechanisms. PG - 1307-1322 LID - 10.19852/j.cnki.jtcm.2026.05.023 [doi] AB - Pulmonary fibrosis (PF) is a fatal respiratory disease characterized by abnormal repair of the pulmonary interstitium and scar formation. Currently, only nintedanib and pirfenidone are approved for clinical use, but their efficacy is limited, and they have significant side effects. The sustained inflammatory microenvironment promotes fibroblast activation and collagen deposition via unresolved inflammation, representing a central pathological mechanism in PF progression. Therefore, anti-inflammatory effects are key in relieving and treating PF. Alkaloids derived from Chinese herbal medicine have become a new hotspot in anti-PF research because of their good anti-inflammatory properties and advantages in multitarget and multipathway approaches. This review elucidates that PF is triggered by multiple factors, such as genetics, the environment, drugs, and infection, and reveals the core mechanism of its pathogenesis from the perspective of the roles of various cell types involved in PF. In this review, we focus on the experimental efficacy and mechanisms of alkaloid drugs that mitigate pulmonary fibrosis through anti-inflammatory actions. We review the research progress of experimental models and signaling pathway regulatory mechanisms to provide a reference for subsequent research. Finally, this review analyzes the transformation bottlenecks faced by alkaloids when applied to PF, such as low active ingredient content, poor target selectivity, and insufficient toxicological and clinical data. Strategies such as structural optimization, nanodelivery, multiomics validation, and real-world research are proposed to overcome these challenges, providing a reference for the development of highly efficient and low-toxicity anti-PF drugs. FAU - Jiamei, Dong AU - Jiamei D AD - Faculty of Chinese Medicine and State Key Laboratory of Mechanism and Quality of Chinese Medicine, Macau University of Science and Technology, Macau 999078, China. AD - Department of Pharmacy, Zhuhai People's Hospital (The Affiliated Hospital of Beijing Institute of Technology, Zhuhai Clinical Medical College of Jinan University), Zhuhai 519000, China. FAU - Jieren, Zhong AU - Jieren Z AD - Department of Radiation Oncology, Zhuhai Clinical Medical College of Jinan University (Zhuhai People's Hospital, The Affiliated Hospital of Beijing Institute of Technology), Zhuhai 519000, China. FAU - Mingqi, Yang AU - Mingqi Y AD - Faculty of Medicine, Macau University of Science and Technology, Macau 999078, China. AD - Outpatient Department, Zhuhai People's Hospital (the Affiliated Hospital of Beijing Institute of Technology, Zhuhai Clinical Medical College of Jinan University), Zhuhai 519000, China. FAU - Jiangkun, Y U AU - Jiangkun YU AD - Department of Nuclear Medicine, Zhuhai People's Hospital (The Affiliated Hospital of Beijing Institute of Technology, Zhuhai Clinical Medical College of Jinan University), Zhuhai 519000, China. FAU - Zhuoling, Zhou AU - Zhuoling Z AD - School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. FAU - Yuhong, Luo AU - Yuhong L AD - Department of Pharmacy, Zhuhai People's Hospital (The Affiliated Hospital of Beijing Institute of Technology, Zhuhai Clinical Medical College of Jinan University), Zhuhai 519000, China. FAU - Ruiqi, Wang AU - Ruiqi W AD - Department of Pharmacy, Zhuhai People's Hospital (The Affiliated Hospital of Beijing Institute of Technology, Zhuhai Clinical Medical College of Jinan University), Zhuhai 519000, China. FAU - Yang, Yang AU - Yang Y AD - Department of Pharmacy, Zhuhai People's Hospital (The Affiliated Hospital of Beijing Institute of Technology, Zhuhai Clinical Medical College of Jinan University), Zhuhai 519000, China. FAU - Haikui, Yang AU - Haikui Y AD - Department of Pharmacy, Zhuhai People's Hospital (The Affiliated Hospital of Beijing Institute of Technology, Zhuhai Clinical Medical College of Jinan University), Zhuhai 519000, China. FAU - Yanyan, Liu AU - Yanyan L AD - Guangdong Provincial Key Laboratory of Tumor Interventional Diagnosis and Treatment, Zhuhai People's Hospital (The Affiliated Hospital of Beijing Institute of Technology, Zhuhai Clinical Medical College of Jinan University), Zhuhai 519000, China. FAU - Jiehua, X U AU - Jiehua XU AD - Faculty of Chinese Medicine and State Key Laboratory of Mechanism and Quality of Chinese Medicine, Macau University of Science and Technology, Macau 999078, China. AD - Department of Nuclear Medicine, Zhuhai People's Hospital (The Affiliated Hospital of Beijing Institute of Technology, Zhuhai Clinical Medical College of Jinan University), Zhuhai 519000, China. FAU - Qibiao, W U AU - Qibiao WU AD - Faculty of Chinese Medicine and State Key Laboratory of Mechanism and Quality of Chinese Medicine, Macau University of Science and Technology, Macau 999078, China. AD - Zhuhai M.U.S.T. Science and Technology Research Institute, Guangdong-Macao In-Depth Cooperation Zone in Hengqin, Zhuhai 519000, China. LA - eng GR - 0048/2023/AFJ/Science and Technology Development Fund, Macau Special Administrative Region: the Molecular Mechanisms of High Metastasis Propensity in Colorectal Cancer with Spleen Deficiency, Damp-Heat, and Blood Stasis Syndrome and the Preventive Role of Xianlian Jiedu Decoction in Preventing Metastasis/ GR - 0164/2023/RIA3/Science and Technology Development Fund, Macau Special Administrative Region: Identification of the Anti-Depressive Mechanism of Ginsenoside by Exploring Its Interaction with Kynurenine 3-Monooxygenase and Effects on the Kynurenine Pathway/ GR - 2021KYQD-03/Zhuhai People's Hospital: Positron Emission Tomography/Computed Tomography Scintigraphy of Hepatocellular Carcinoma with Aptamer JHIT2e And Its Targeted Molecular/ GR - DDYL-A-KT-20250519-0058/Beijing Dadi Medical Charity Foundation: Population Pharmacokinetics of Meropenem in Patients with SevereInfections/ GR - 2024A1515220042/Basic and Applied Basic Research Project of Guangdong Province: Application Research of Multiple Biomarker Detection Based on Electrode Array and Electrochemiluminescence Technology in Early Diagnosis of Liver Cancer/ GR - 2024A03J0377/Science and Technology Planning Project of Guangzhou: the Role and Mechanism of Asproin in Liver Fibrosis Associated with Non-Alcoholic Fatty Liver Disease/ GR - 82304589/National Natural Science Foundation of China: Asporin Promotes Hepatic Fibrosis by Activating Cyclin-dependent Kinase 1 Related Mitochondrial Homeostasis Imbalance of Hepatic Stellate Cells/ PT - Journal Article PT - Review PL - China TA - J Tradit Chin Med JT - Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan JID - 8211546 RN - 0 (Alkaloids) RN - 0 (Drugs, Chinese Herbal) RN - 0 (Anti-Inflammatory Agents) SB - IM MH - Humans MH - *Alkaloids/chemistry/administration & dosage/therapeutic use MH - Animals MH - *Pulmonary Fibrosis/drug therapy/immunology/genetics MH - *Drugs, Chinese Herbal/chemistry/administration & dosage/therapeutic use MH - *Anti-Inflammatory Agents/administration & dosage/chemistry/therapeutic use MH - Medicine, Chinese Traditional MH - Signal Transduction/drug effects OTO - NOTNLM OT - antifibrosis OT - inflammation OT - mechanism of action OT - pulmonary fibrosis OT - review OT - traditional Chinese medicinal alkaloids EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:24 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/10/30 01:24 [entrez] AID - 1792400688970-1466439118 [pii] AID - 10.19852/j.cnki.jtcm.2026.05.023 [doi] PST - ppublish SO - J Tradit Chin Med. 2026 Oct;46(5):1307-1322. doi: 10.19852/j.cnki.jtcm.2026.05.023. PMID- 42958679 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2589-451X (Electronic) IS - 0255-2922 (Linking) VI - 46 IP - 5 DP - 2026 Oct TI - Assessment of damp-heat impeding syndrome in rheumatoid arthritis: development and validation of a diagnostic scale. PG - 1286-1294 LID - 10.19852/j.cnki.jtcm.2026.05.021 [doi] AB - OBJECTIVE: To develop a novel quantitative scale for Damp-Heat Impeding Syndrome (DHIS) in rheumatoid arthritis (RA) and to enhance diagnostic reliability through a combined consensus- and data-driven approach grounded in Traditional Chinese Medicine (TCM) principles, thereby addressing the limitations of existing expert-consensus-based tools. METHODS: Scale items were selected from the China Rheumatoid Arthritis Registry of Patients with Chinese Medicine (CERTAIN) database, a prospective, multicenter, observational disease registry for RA patients receiving TCM treatment, and refined through expert consultation. Based on TCM theory and clinical guidance, items were categorized into three domains: disease-related, heat-related, and dampness-related. A two-stage statistical method was applied to estimate item scores and determine cutoff values for each domain. According to TCM diagnostic principles, DHIS is identified only when the scores in all three domains exceed their respective thresholds. To enhance clinical applicability, two versions of the scale were developed: one including pulse manifestations and one excluding them. RESULTS: The dataset was divided based on follow-up time into a development set (n = 6680) and a validation set (n = 3882), both from the CERTAIN database. A DHIS diagnostic scale was developed with two versions: Version 1 included pulse manifestations (11 items), and Version 2 excluded them (10 items). The scale comprised three domains: disease-related, heat-related, and dampness-related. Key symptoms included joint pain as well as tongue and pulse features such as yellow tongue coating and slippery pulse. Secondary symptoms included sticky stool and greasy tongue coating. In the development set, Version 1 achieved a Youden index of 0.83 and sensitivity of 0.89; in the validation set, it achieved 0.78 and 0.86, respectively. Cronbach's alpha was 0.78 for Version 1 and 0.75 for Version 2. CONCLUSIONS: Both versions of the DHIS diagnostic scale demonstrated good internal consistency and strong diagnostic performance, supporting their utility in identifying DHIS among RA patient. FAU - Yanting, L I AU - Yanting LI AD - Department of Rheumatology, Guang'anmen Hospital, Beijing 100053, China. FAU - Ao, Sun AU - Ao S AD - Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China. FAU - Xun, Gong AU - Xun G AD - Department of Rheumatology, Guang'anmen Hospital, Beijing 100053, China. FAU - Xiaopo, Tang AU - Xiaopo T AD - Department of Rheumatology, Guang'anmen Hospital, Beijing 100053, China. FAU - Weixiang, Liu AU - Weixiang L AD - Department of Rheumatology, Guang'anmen Hospital, Beijing 100053, China. FAU - Congmin, Xia AU - Congmin X AD - Department of Rheumatology, Guang'anmen Hospital, Beijing 100053, China. FAU - Jian, Wang AU - Jian W AD - Department of Rheumatology, Guang'anmen Hospital, Beijing 100053, China. FAU - Mengge, Song AU - Mengge S AD - Department of Rheumatology, Guang'anmen Hospital, Beijing 100053, China. FAU - Qiuwei, Peng AU - Qiuwei P AD - Department of Rheumatology, Guang'anmen Hospital, Beijing 100053, China. FAU - Xieli, M A AU - Xieli MA AD - Department of Rheumatology, Guang'anmen Hospital, Beijing 100053, China. FAU - Xiaohua, Zhou AU - Xiaohua Z AD - Beijing International Center for Mathematical Research, Peking University, Beijing 100871, China. FAU - Quan, Jiang AU - Quan J AD - Department of Rheumatology, Guang'anmen Hospital, Beijing 100053, China. LA - eng GR - HLCMHPP2023087/High Level Chinese Medical Hospital Promotion Project: Pattern-based Prescription Rules and Optimal Herbal Formulas in Chinese Medicine for the Treatment of Bone Erosion in Rheumatoid Arthritis: a Systematic Study/ PT - Journal Article PT - Validation Study PL - China TA - J Tradit Chin Med JT - Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan JID - 8211546 SB - IM MH - Humans MH - *Arthritis, Rheumatoid/diagnosis/physiopathology MH - Female MH - Middle Aged MH - Male MH - Medicine, Chinese Traditional MH - Adult MH - Aged MH - Prospective Studies MH - Hot Temperature OTO - NOTNLM OT - arthritis, rheumatoid OT - damp-heat impeding syndrome OT - diagnostic scale OT - sensitivity and specificity EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:24 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/10/30 01:24 [entrez] AID - 1792400675983-607972479 [pii] AID - 10.19852/j.cnki.jtcm.2026.05.021 [doi] PST - ppublish SO - J Tradit Chin Med. 2026 Oct;46(5):1286-1294. doi: 10.19852/j.cnki.jtcm.2026.05.021. PMID- 42958677 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2589-451X (Electronic) IS - 0255-2922 (Linking) VI - 46 IP - 5 DP - 2026 Oct TI - Effect of Fuyuan Tongluo acupuncture on neurological function and serum chitinase-3-like protein 1, platelet-activating factor and osteopontin in patients with acute cerebral infarction and its prognostic factors. PG - 1266-1275 LID - 10.19852/j.cnki.jtcm.2026.05.019 [doi] AB - OBJECTIVE: To analyze the effect of Fuyuan Tongluo acupuncture on neurological function, serum chitinase-3-like protein 1 (YKL-40), platelet-activating factor (PAF) and osteopontin (OPN) in patients with acute cerebral infarction and its prognostic factors, so as to provide a scientific basis for clinical judgment of disease condition and optimization of treatment plan. METHODS: A total of 102 patients with acute cerebral infarction (ACI) who were hospitalized in the Department of Neurology of Hai'an Hospital of Traditional Chinese Medicine between January 2021 and June 2022 and diagnosed by head computed tomography (CT) and/or magnetic resonance imaging (MRI) were selected as the ACI group, and 40 healthy subjects were selected as the healthy control group. According to the treatment methods documented in the medical records, 102 patients with ACI were divided into group A (50 cases, treated with conventional Western Medicine combined with Fuyuan Tongluo acupuncture) and group B (52 cases, treated with conventional Western Medicine). The clinical efficacy, serum levels of YKL-40, PAF, OPN, and carotid intima-media thickness (IMT) were compared, and the correlation between the levels of the above serum indexes and IMT was analyzed. According to the modified Rankin scale score at 90 d after treatment, the combined group was divided into good prognosis group and poor prognosis group. The baseline data, serum YKL-40, PAF and OPN levels were compared between the two groups, the influencing factors of poor prognosis of ACI were explored, and the predictive value of serum YKL-40, PAF and OPN levels for poor short-term prognosis of ACI was evaluated. RESULTS: The short-term total effective rate of group A was 90.00%, which was significantly higher than that of group B (69.23%) (P <0.05). After treatment, the National Institutes of Health Stroke Scale (NIHSS) score, Mini-Mental State Examination (MMSE) score and Barthel index of the two groups were significantly higher than those before treatment, and the scores in group A were higher than those in group B (P <0.05). After treatment, the serum levels of YKL-40, PAF and OPN in the two groups were significantly lower than those before treatment, and those in group A were lower than those in group B (P <0.05). The levels of serum YKL-40, PAF, OPN and IMT in ACI group were higher than those in healthy control group (P <0.05). Serum YKL-40 (r = 0.426, P <0.001), PAF (r = 0.588, P <0.001), and OPN (r = 0.474, P <0.001) levels were positively correlated with IMT in ACI patients, as determined by Pearson correlation analysis. In the poor prognosis group, the levels of IMT, the proportion of unstable plaques, and the concentrations of YKL-40, PAF, and OPN were significantly higher than those in the good prognosis group (P <0.05). Multivariate logistic regression analysis showed that serum YKL-40 and PAF were independently associated with poor short-term prognosis, whereas IMT and OPN did not reach statistical significance. receiver operating characteristic (ROC) analysis demonstrated that the area under the curve (AUC) values for YKL-40, PAF, and OPN were 0.833, 0.972, and 0.893 respectively. A combined biomarker model showed improved discriminative performance for short-term prognosis. CONCLUSION: The combination of Fuyuan Tongluo acupuncture and conventional Western Medicine was associated with improvements in the rehabilitation of patients with acute cerebral infarction, as evidenced by reduced serum levels of YKL-40, PAF, and OPN, along with improvements in neurofunctional, cognitive, and daily living abilities. However, these observed changes may not be solely attributable to acupuncture alone and could also be associated with natural disease recovery and standard treatment. Therefore, further validation through large-sample, multi-center studies is warranted. FAU - Xiaoming, X U AU - Xiaoming XU AD - Hai'an Hospital of Traditional Chinese Medicine, Haian 226600, China. FAU - Xiaoping, M A AU - Xiaoping MA AD - Hai'an Hospital of Traditional Chinese Medicine, Haian 226600, China. LA - eng GR - TZYK202407/Clinical Study on the Treatment of Erectile Dysfunction in Young Stroke Patients Using Deep Acupuncture at Ciliao Point Combined with Sexual Rehabilitation Therapy Nantong City Traditional Chinese Medicine Medical Alliance Research Project/ PT - Journal Article PL - China TA - J Tradit Chin Med JT - Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan JID - 8211546 RN - 0 (Chitinase-3-Like Protein 1) RN - 106441-73-0 (Osteopontin) RN - 0 (Platelet Activating Factor) RN - 0 (CHI3L1 protein, human) SB - IM MH - Humans MH - *Chitinase-3-Like Protein 1/blood MH - Male MH - Female MH - *Cerebral Infarction/therapy/blood/physiopathology/diagnosis MH - Middle Aged MH - *Osteopontin/blood MH - Aged MH - Prognosis MH - *Platelet Activating Factor/metabolism MH - Adult OTO - NOTNLM OT - cerebral infarction OT - chitinase protein-40 OT - osteopontin OT - platelet activating factor OT - poor prognosis EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:24 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/10/30 01:24 [entrez] AID - 1792400662348-1610925486 [pii] AID - 10.19852/j.cnki.jtcm.2026.05.019 [doi] PST - ppublish SO - J Tradit Chin Med. 2026 Oct;46(5):1266-1275. doi: 10.19852/j.cnki.jtcm.2026.05.019. PMID- 42958672 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2589-451X (Electronic) IS - 0255-2922 (Linking) VI - 46 IP - 5 DP - 2026 Oct TI - Integrative network pharmacology and experimental validation to decipher the anti-angiogenic mechanisms of Xuandan Sanjie decotion in adenomyosis. PG - 1218-1229 LID - 10.19852/j.cnki.jtcm.20260514.001 [doi] AB - OBJECTIVE: To investigate the mechanisms by which Xuandan Sanjie decoction (, XSD) inhibits angiogenesis in adenomyosis (AM), using both AM-derived primary endometrial stromal cells (ESCs) and a mouse model. METHODS: Core components and targets of XSD for AM treatment were predicted using multiple databases. Gene ontology and kyoto encyclopedia of genes and genomes pathway analyses were used to elucidate the potential mechanisms. Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was applied to identify chemical constituents of XSD. A cobalt (II) chloride (CoCl(2))-induced hypoxia-mimicking ESC model and an AM mouse model were established to experimentally validate the anti-angiogenic effects of XSD. RESULTS: According to bioinformatics analysis, hypoxia signaling and angiogenesis are crucial for AM progression. Twenty chemical constituents of the 106 compounds identified from the Traditional Chinese Medicine systems pharmacology database and analysis platform database were validated by UHPLC-MS/MS. Network pharmacology identified protein kinase B alpha, epidermal growth factor receptor, hypoxia inducible factor-1alpha (HIF-1alpha), and estrogen receptor 1 (ESR1) as core targets. According to in vitro and in vivo experiments, XSD exhibits anti-angiogenic properties through ESR1/G protein-coupled estrogen receptor (GPER)-mediated HIF-1alpha signaling. CONCLUSION: Our study confirms that XSD inhibits angiogenesis in AM viaESR1/GPER-mediated HIF-1alpha signaling. These findings elucidate the molecular mechanisms underlying XSD's therapeutic effects for abnormal uterine bleeding associated with adenomyosis, offering new therapeutic strategies for this condition. FAU - Lin, Gan AU - Lin G AD - Department of Obstetrics and Gynecology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China. AD - Department of Gynecology, Affiliated Jiangning Hospital of Chinese Medicine, China Pharmaceutical University, Nanjing 211100, China. FAU - Fangfang, Zhou AU - Fangfang Z AD - Department of Obstetrics and Gynecology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China. FAU - Yingying, Qiu AU - Yingying Q AD - Department of Gynecology, Affiliated Xuzhou Municipal Hospital of Xuzhou Medical University, Xuzhou 221112, China. FAU - Meihua, Huang AU - Meihua H AD - Department of Obstetrics and Gynecology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China. FAU - Zhenli, L I AU - Zhenli LI AD - Department of Obstetrics and Gynecology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China. FAU - Tao, Gui AU - Tao G AD - Department of Obstetrics and Gynecology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China. LA - eng GR - 81403321/National Natural Science Foundation of China: To Explore the Effect and Mechanism of Yiqi Sanjie Decoction in Relieving Pain of Adenomyosis Based on the Nerve Growth Factor/Extracellular Regulated Protein Kinases pathway/ GR - MS2023039/Science and Technology Project of Jiangsu Provincial Administration of Traditional Chinese Medicine: Study on the Mechanism of Xuandan Sanjie Decoction Regulating the Estradiol/G-protein Coupled Estrogen Receptor 1 /Hypoxia Inducible Factor-1alpha Pathway of Endometrial Mesenchymal Stem Cells to Inhibit Angiogenesis in Uterine Adenomyosis/ GR - MS2023079/Science and Technology Project of Jiangsu Provincial Administration of Traditional Chinese Medicine: Study on the Mechanism of the Formula of Tonifying Kidney and Activating Blood Circulation and Dissipating Blood Stasis in Regulating Macrophage Polarization and Improving Endometrial Receptivity Based on the miRNA-103a-3p/Dual Specificity Protein Phosphatase 2/Extracellular Regulated Protein Kinases Axis/ GR - ZD202419/Science and Technology Project of Jiangsu Provincial Administration of Traditional Chinese Medicine: Based on the Study of Inducing Macrophage Polarization by Endometrial Mesenchymal Stem Cells, Exploring the Mechanism of the "Supplement Kidney and Activate Blood, Dispel Stasis" Formula in Inhibiting the Invasion and Migration of Endometrium in Uterine Adenomyosis/ GR - 2024003S/Nanjing Jiangning District Science and Technology Benefit People Plan Project: In vitro Mechanism of Xuandan Sanjie Decoction Inhibiting Adenomyosis Angiogenesis through the Estradiol/Hypoxia Inducible Factor-1alpha Pathway/ GR - CYTF2024025/Jiangsu Chinese Medicine Society: Mechanistic Study of Xuandan Sanjie Decoction in the Treatment of Adenomyosis via Inhibiting Transferrin Receptor Protein 1-mediated Ferroptosis/ GR - YJZD2025001/Research Project Funding from Affiliated Jiangning Hospital of Chinese Medicine, China Pharmaceutical University: Xuandan Sanjie Decoction Suppresses Adenomyosis by Modulating Ferroptosis through Transferrin Receptor/Nuclear Receptor Coactivator 4-mediated Ferritinophagy/ GR - KC25074/Science and Technology Program of Xuzhou: Research and Development of Intervention Technology for Embryo Implantation Disorders in Adenomyosis Based on a 3D Endometrium-Embryo Co-culture Platform/ PT - Journal Article PL - China TA - J Tradit Chin Med JT - Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan JID - 8211546 RN - 0 (Drugs, Chinese Herbal) RN - 0 (Angiogenesis Inhibitors) RN - 0 (Hypoxia-Inducible Factor 1, alpha Subunit) RN - 0 (Receptors, G-Protein-Coupled) SB - IM MH - *Adenomyosis/drug therapy/genetics/metabolism/physiopathology MH - Animals MH - Female MH - *Drugs, Chinese Herbal/administration & dosage/chemistry MH - Humans MH - Mice MH - *Angiogenesis Inhibitors/administration & dosage/chemistry MH - Network Pharmacology MH - Tandem Mass Spectrometry MH - Angiogenesis/drug effects MH - Hypoxia-Inducible Factor 1, alpha Subunit/metabolism/genetics MH - Signal Transduction/drug effects MH - Receptors, G-Protein-Coupled/metabolism/genetics MH - *Neovascularization, Pathologic/genetics/drug therapy/metabolism OTO - NOTNLM OT - Xuandan Sanjie decoction OT - adenomyosis OT - angiogenesis OT - endometrial stromal cells OT - network pharmacology EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:24 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/10/30 01:24 [entrez] AID - 1792400626133-989170880 [pii] AID - 10.19852/j.cnki.jtcm.20260514.001 [doi] PST - ppublish SO - J Tradit Chin Med. 2026 Oct;46(5):1218-1229. doi: 10.19852/j.cnki.jtcm.20260514.001. PMID- 42958656 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1941-5923 (Electronic) IS - 1941-5923 (Linking) VI - 27 DP - 2026 Oct 30 TI - Tibial Post Fracture in a Posterior-Stabilized Knee Prosthesis: A Case Report and Review of the Literature. PG - e954794 LID - 10.12659/AJCR.954794 [doi] AB - BACKGROUND Tibial post fracture is an uncommon but likely underrecognized complication of posterior-stabilized (PS) total knee arthroplasty (TKA) that often remains undiagnosed because conventional radiographs may be unrevealing. We report a late tibial post fracture in a previously well-functioning PS TKA, highlighting the role of dynamic fluoroscopy in raising preoperative suspicion and the successful use of isolated polyethylene insert exchange in a carefully selected patient. CASE REPORT A 92-year-old man presented with acute mechanical pain, recurrent clicking, and subjective instability 16 years after primary PS TKA following a low-energy flexion-rotation movement. Plain radiographs showed no evidence of periprosthetic fracture, component loosening, or implant failure. Dynamic fluoroscopy during flexion, which reproduced the patient's symptoms, demonstrated abnormal anterior translation of the femoral component relative to the tibial insert during knee flexion, raising suspicion of cam-post mechanism failure. Revision surgery confirmed a fracture of the polyethylene tibial post with chronic wear at its base; the metallic components remained well fixed, without gross malposition. Isolated polyethylene insert exchange with a thicker insert restored intraoperative stability. At 4.5 months postoperatively, the patient demonstrated complete resolution of mechanical symptoms, with full extension, 100 degrees to 110 degrees of flexion, and preserved varus-valgus and anteroposterior stability. CONCLUSIONS This case illustrates how dynamic fluoroscopy may raise preoperative suspicion of tibial post fracture in selected patients with symptomatic late PS TKA and inconclusive conventional radiographs. In our patient, isolated polyethylene insert exchange was effective after confirmation of preserved component fixation, alignment, and overall stability, supporting its use in appropriately selected cases. FAU - Avila Lozano, Cesar Abraham AU - Avila Lozano CA AD - ABC Medical Centre, Sports Medicine, Adult Hip & Knee Reconstruction, Mexico City, Mexico. FAU - Jimenez-Aroche, Cesar Alejandro AU - Jimenez-Aroche CA AUID- ORCID: 0000-0001-5233-2662 AD - ABC Medical Centre, Sports Medicine, Adult Hip & Knee Reconstruction, Mexico City, Mexico. FAU - Kerbel Sutton, Jacobo AU - Kerbel Sutton J AD - ABC Medical Centre, Sports Medicine, Adult Hip & Knee Reconstruction, Mexico City, Mexico. FAU - Ponce de Leon Sandoval, Iturbide Andres AU - Ponce de Leon Sandoval IA AD - ABC Medical Centre, Sports Medicine, Adult Hip & Knee Reconstruction, Mexico City, Mexico. FAU - Miranda-Mayen, Jose Eduardo AU - Miranda-Mayen JE AUID- ORCID: 0009-0000-1672-8491 AD - ABC Medical Centre, Sports Medicine, Adult Hip & Knee Reconstruction, Mexico City, Mexico. FAU - Kahana-Rojkind, Ady Haim AU - Kahana-Rojkind AH AD - ABC Medical Centre, Sports Medicine, Adult Hip & Knee Reconstruction, Mexico City, Mexico. FAU - Gomez Amezcua, Juan Jose AU - Gomez Amezcua JJ AD - ABC Medical Centre, Sports Medicine, Adult Hip & Knee Reconstruction, Mexico City, Mexico. LA - eng PT - Case Reports PT - Journal Article PT - Review DEP - 20261030 PL - United States TA - Am J Case Rep JT - The American journal of case reports JID - 101489566 SB - IM MH - Humans MH - Male MH - *Arthroplasty, Replacement, Knee/adverse effects MH - Aged, 80 and over MH - *Knee Prosthesis/adverse effects MH - *Tibial Fractures/etiology/surgery/diagnostic imaging MH - Reoperation MH - Prosthesis Failure MH - Fluoroscopy EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:24 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/10/30 01:24 [entrez] AID - 954794 [pii] AID - 10.12659/AJCR.954794 [doi] PST - epublish SO - Am J Case Rep. 2026 Oct 30;27:e954794. doi: 10.12659/AJCR.954794. PMID- 42958650 OWN - NLM STAT- Publisher LR - 20261030 IS - 2753-670X (Electronic) IS - 2753-670X (Linking) DP - 2026 Oct 29 TI - Postoperative Vocal fold mobility impairment after thoracic aortic surgery: A systematic review and meta-analysis of incidence, anatomy, and ascertainment. LID - ivag291 [pii] LID - 10.1093/icvts/ivag291 [doi] AB - OBJECTIVES: Postoperative vocal fold mobility impairment (VFMI) after thoracic aortic surgery is reported inconsistently because operative anatomy, outcome definitions and postoperative ascertainment vary substantially. We evaluated variation in observed VFMI, its relationship with anatomical exposure and ascertainment, and its reported clinical consequences. METHODS: PubMed, Embase and MEDLINE (Ovid) were searched from inception to July 2026 for adult studies reporting VFMI after open or hybrid thoracic aortic surgery. Random-effects meta-analysis was restricted to symptom-triggered, instrumentally confirmed cohorts undergoing direct open thoracic-aortic reconstruction. Risk of bias was assessed using QUIPS and JBI guidance, and certainty using GRADE. RESULTS: Thirty-nine observational studies were included. Eleven symptom-triggered, instrumentally confirmed cohorts (2,082 patients; 263 events) contributed to the primary synthesis. Pooled observed VFMI was 14.9% (95% CI 9.4-21.5%), but heterogeneity was substantial (I(2) 92.7%, 95% CI 89.7-94.6%) and the 95% prediction interval was 0.3-43.3%. Study-level incidence ranged from 4.0% to 38.8%. Greater anatomical extent, particularly distal arch or descending exposure, was consistently associated with higher observed VFMI, whereas specific left-subclavian-proximate estimates were based on limited observational evidence. VFMI was also associated with pulmonary morbidity, airway intervention and prolonged hospitalisation. CONCLUSION: A single pooled incidence does not adequately describe postoperative VFMI after thoracic aortic surgery. Observed risk varies markedly with anatomical exposure and ascertainment strategy. The evidence supports targeted postoperative laryngeal assessment in symptomatic patients and highlights the need to evaluate routine screening in selected high-risk distal arch and descending procedures. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. FAU - Farooq, Omar AU - Farooq O AD - Royal Blackburn Teaching Hospital, East Lancashire Hospitals NHS Trust, Blackburn, England, UK. FAU - Jamileh, Eyad AU - Jamileh E AD - School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, Northern Ireland, UK. AD - Furness General Hospital, University Hospitals of Morecambe Bay NHS Foundation Trust, Cumbria, UK. FAU - Muhammad, Fahad AU - Muhammad F AD - School of Medicine and Dentistry, Barts and The London, Queen Mary University of London, London, UK. FAU - Nawaz, Alishba AU - Nawaz A AD - Maidstone and Tunbridge Wells Hospital Trust, Maidstone, Kent, England, UK. FAU - Abbas, Zaheer AU - Abbas Z AD - School of Medicine, University of Central Lancashire, School of Medicine, UK. FAU - Layton, Georgia AU - Layton G AD - Department of Cardiac Surgery, University Hospitals of Leicester NHS Trust, Leicester, England, UK. AD - Division of Cardiovascular Sciences, Clinical Science Wing, University of Leicester, Glenfield Hospital, Leicester, UK. FAU - Zakkar, Mustafa AU - Zakkar M AD - Department of Cardiac Surgery, University Hospitals of Leicester NHS Trust, Leicester, England, UK. AD - Division of Cardiovascular Sciences, Clinical Science Wing, University of Leicester, Glenfield Hospital, Leicester, UK. FAU - Antoun, Ibrahim AU - Antoun I AD - Division of Cardiovascular Sciences, Clinical Science Wing, University of Leicester, Glenfield Hospital, Leicester, UK. AD - Department of Cardiology, University Hospitals of Leicester NHS Trust, Glenfield Hospital, Leicester, UK. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Interdiscip Cardiovasc Thorac Surg JT - Interdisciplinary cardiovascular and thoracic surgery JID - 9918540787006676 SB - IM OTO - NOTNLM OT - Vocal fold mobility impairment OT - aortic arch surgery OT - ascertainment OT - recurrent laryngeal nerve OT - thoracic aortic surgery EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:23 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/09/12 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/30 01:23 [entrez] AID - 9004986 [pii] AID - 10.1093/icvts/ivag291 [doi] PST - aheadofprint SO - Interdiscip Cardiovasc Thorac Surg. 2026 Oct 29:ivag291. doi: 10.1093/icvts/ivag291. PMID- 42958648 OWN - NLM STAT- Publisher LR - 20261030 IS - 1557-7740 (Electronic) IS - 1557-7740 (Linking) DP - 2026 Oct 29 TI - Intensity of End-of-Life Care Among Patients with Cerebral Palsy: A 12-Year Nationwide Study. PG - 10966218261502535 LID - 10.1177/10966218261502535 [doi] AB - BACKGROUND: For noncancer neurological conditions, uncertain trajectories complicate prognostication and end-of-life (EOL) decision-making. Similar challenges may arise in some patients with cerebral palsy (CP), yet population-level evidence on EOL care remains limited. OBJECTIVES: To characterize EOL care among patients with CP across the lifespan by describing in-hospital deaths, examining care intensity during the last 7 days of life, and identifying associated patient-level and hospital-level factors. DESIGN: Nationwide retrospective cohort study using the Japanese Diagnosis Procedure Combination/Per-Diem Payment System inpatient database, April 2010-March 2022. SETTING/SUBJECTS: Patients aged >/=1 year with CP who died in participating hospitals in Japan, categorized as children (1-18 years), young adults (19-39 years), and adults (>/=40 years). MEASUREMENTS: Intensive EOL care during the last 7 days of life was assessed using seven interventions. High-intensity care was defined as >/=3 interventions. Multivariable analyses focused on children. RESULTS: We analyzed 2482 patients; median age was 49 years. EOL care intensity was highest among children; 47.7% received cardiopulmonary resuscitation (CPR) and 52.3% received high-intensity care. Among children, shorter hospital stays were associated with CPR and high-intensity care, and nonpediatric departments with CPR. Deaths were widely distributed across hospitals; in each age group, more than half of hospitals experienced only one death over 12 years. CONCLUSIONS: EOL care intensity was highest among children, with shorter hospital stays and treatment in nonpediatric departments associated with selected intensive interventions. Deaths were widely distributed across hospitals. These findings highlight the importance of continuity of decision-making support and information sharing across departments and institutions. FAU - Kuratani, Saori AU - Kuratani S AUID- ORCID: 0009-0007-8697-5157 AD - Division of Palliative Care, Department of General Pediatrics and Interdisciplinary Medicine, National Center for Child Health and Development, Tokyo, Japan. AD - Department of Pediatrics, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan. FAU - Yotani, Nobuyuki AU - Yotani N AD - Division of Palliative Care, Department of General Pediatrics and Interdisciplinary Medicine, National Center for Child Health and Development, Tokyo, Japan. FAU - Shinjo, Daisuke AU - Shinjo D AD - Department of Health Policy and Informatics, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Tokyo, Japan. FAU - Fushimi, Kiyohide AU - Fushimi K AD - Department of Health Policy and Informatics, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Tokyo, Japan. FAU - Miyakoshi, Chisato AU - Miyakoshi C AD - Department of Research Support, Center for Clinical Research and Innovation, Kobe City Medical Center General Hospital, Kobe, Japan. FAU - Hamazaki, Takashi AU - Hamazaki T AD - Department of Pediatrics, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Palliat Med JT - Journal of palliative medicine JID - 9808462 SB - IM OTO - NOTNLM OT - advance care planning OT - cardiopulmonary resuscitation OT - cerebral palsy OT - end-of-life care OT - neurological disorders OT - palliative care EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:23 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/10/30 01:23 [entrez] AID - 10.1177/10966218261502535 [doi] PST - aheadofprint SO - J Palliat Med. 2026 Oct 29:10966218261502535. doi: 10.1177/10966218261502535. PMID- 42958643 OWN - NLM STAT- Publisher LR - 20261030 IS - 1432-2323 (Electronic) IS - 0364-2313 (Linking) DP - 2026 Oct 29 TI - Development of a Prognostic Score for Papillary Thyroid Carcinoma Incorporating Ki-67 Labeling Index: MASSIN-K. LID - 10.1002/wjs.70637 [doi] AB - BACKGROUND: We previously demonstrated that the Ki-67 labeling index (LI), a marker of cell proliferation, strongly predicts prognosis in patients with papillary thyroid carcinoma (PTC). We subsequently developed a prognostic scoring system incorporating Ki-67 LI. METHODS: We enrolled 8540 patients with PTC who underwent curative surgery (median follow-up, 8.7 years). Multivariate Cox regression analysis identified prognostic factors, and regression coefficients from the final models were rounded to generate integer-based scores. RESULTS: To date, 368, 125, and 22 patients experienced local recurrence (LR), distant recurrence (DR), and carcinoma-related death, respectively. Scores were assigned for age, tumor size, N factor, M factor, Ki-67 LI, and extrathyroidal/extranodal invasion (Ex). The LR score comprised sex (0 or 1), age (0, 1, or 2), T (0 or 2), N (0, 2, or 4), Ki-67 (0 or 4), and Ex (0, 1, or 2). Ten-year LR-free survival rates for low (/= 5) scores were 98.1%, 95.2%, and 84.5%, respectively (p < 0.001). The DR score comprised sex (0 or 1), age (0 or 3), T (0 or 3), N (0, 2, or 4), Ki-67 (0 or 5), and Ex (0, 3, or 6). Ten-year DR-free survival rates for low (/= 10) scores were 99.6%, 97.9%, and 85.8%, respectively (p < 0.01). The cause-specific survival (CSS) score for the entire series comprised sex (0 or 2), age (0 or 4), N (0, 1, or 2), M (0 or 4), Ki-67 (0 or 3), and Ex (0, 1, or 2). Ten-year CSS rates for low (/= 6) scores were 100%, 99.8%, and 97.1%, respectively (p < 0.01). In M-negative patients, the CSS score comprised sex (0 or 1), age (0 or 3), N (0, 1, or 2), Ki-67 (0 or 3), and Ex (0, 1, or 2). The 10-year CSS rate for high-score (>/= 6) patients was 96.9%, significantly lower than those for low- and intermediate-score patients (100% and 99.8%, respectively; p < 0.01). CONCLUSIONS: MASSIN-K, a prognostic scoring system incorporating Ki-67 LI, showed good prognostic discrimination in patients with PTC. External validation is required as a further study. CI - (c) 2026 The Author(s). World Journal of Surgery published by John Wiley & Sons Ltd on behalf of International Society of Surgery/Societe Internationale de Chirurgie (ISS/SIC). FAU - Ito, Yasuhiro AU - Ito Y AUID- ORCID: 0000-0002-2069-4658 AD - Department of Surgery, Kuma Hospital, Kobe, Hyogo, Japan. FAU - Yamamoto, Masashi AU - Yamamoto M AUID- ORCID: 0000-0002-9447-6602 AD - Department of Head and Neck Surgery, Kuma Hospital, Kobe, Hyogo, Japan. FAU - Hirokawa, Mitsuyoshi AU - Hirokawa M AUID- ORCID: 0000-0001-5558-7142 AD - Department of Diagnostic Pathology, Kuma Hospital, Kobe, Hyogo, Japan. FAU - Masuoka, Hiroo AU - Masuoka H AUID- ORCID: 0000-0001-9850-4159 AD - Department of Surgery, Kuma Hospital, Kobe, Hyogo, Japan. FAU - Kihara, Minoru AU - Kihara M AUID- ORCID: 0000-0003-1778-5177 AD - Department of Surgery, Kuma Hospital, Kobe, Hyogo, Japan. FAU - Miya, Akihiro AU - Miya A AUID- ORCID: 0000-0003-1891-7135 AD - Department of Surgery, Kuma Hospital, Kobe, Hyogo, Japan. FAU - Miyauchi, Akira AU - Miyauchi A AUID- ORCID: 0000-0003-0041-197X AD - Department of Surgery, Kuma Hospital, Kobe, Hyogo, Japan. FAU - Akamizu, Takashi AU - Akamizu T AUID- ORCID: 0000-0003-4960-8271 AD - Department of Internal Medicine, Kuma Hospital, Kobe, Hyogo, Japan. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - World J Surg JT - World journal of surgery JID - 7704052 SB - IM OTO - NOTNLM OT - Ki-67 OT - papillary thyroid carcinoma OT - prognosis OT - scoring system EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:23 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/09/30 00:00 [revised] PHST- 2026/06/25 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/30 01:23 [entrez] AID - 10.1002/wjs.70637 [doi] PST - aheadofprint SO - World J Surg. 2026 Oct 29. doi: 10.1002/wjs.70637. PMID- 42958641 OWN - NLM STAT- Publisher LR - 20261030 IS - 1537-2995 (Electronic) IS - 0041-1132 (Linking) DP - 2026 Oct 29 TI - Malaria in a US resident blood donor following a 3-month travel deferral. LID - 10.1111/trf.70452 [doi] AB - BACKGROUND: The United States defers blood donations from people who have recently traveled to or lived in malaria-endemic areas. While transfusion-transmitted malaria is rare, it primarily occurs when the donor is a previous resident of a malaria-endemic area infected with Plasmodium parasites. In 2020, the travel deferral period was revised for US residents from 1 year to 3 months after leaving a malaria-endemic area. CASE REPORT: A single unit of packed red blood cells (pRBC) was produced from a whole blood donation collected from an asymptomatic donor 109 days after return from travel to East Africa. The donor was born and resided in the United States but repeatedly traveled to the region, reportedly took malaria chemoprophylaxis, and denied previous malaria illness. Eleven days post-donation, the donor presented to a healthcare provider with flu-like symptoms and was diagnosed with Plasmodium ovale malaria 18 days after donation. Four days after diagnosis, the blood establishment was notified of the donor's malaria diagnosis; however, the pRBC had been transfused the previous day. The recipient of the pRBC was placed on treatment, repeatedly tested negative for Plasmodium parasites, and remained asymptomatic. CONCLUSIONS: We describe a near-miss of transfusion-transmitted P. ovale malaria associated with a US-resident donor despite a 3-month travel deferral. P. ovale and Plasmodium vivax forms of malaria can cause disease relapse months to years after initial infection. Additional strategies for enhancing blood safety include testing of donors with molecular assays, pathogen reduction of select blood components, and a longer travel deferral window. CI - (c) 2026 AABB. FAU - Boop, Sarah AU - Boop S AD - Tennessee Department of Health, Johnson City, Tennessee, USA. FAU - Finnis, Matthew C AU - Finnis MC AD - East Tennessee State University, College of Medicine, Johnson City, Tennessee, USA. FAU - Kirschke, David AU - Kirschke D AD - Tennessee Department of Health, Johnson City, Tennessee, USA. FAU - Moncayo, Abelardo AU - Moncayo A AD - Tennessee Department of Health, Johnson City, Tennessee, USA. FAU - Sundin, Ashley AU - Sundin A AUID- ORCID: 0009-0008-1797-8255 AD - East Tennessee State University, College of Medicine, Johnson City, Tennessee, USA. FAU - Thompson, Jefferson AU - Thompson J AD - East Tennessee State University, College of Medicine, Johnson City, Tennessee, USA. FAU - Vedantam, Neethu AU - Vedantam N AD - East Tennessee State University, College of Medicine, Johnson City, Tennessee, USA. FAU - Vedantam, Venkata AU - Vedantam V AD - East Tennessee State University, College of Medicine, Johnson City, Tennessee, USA. FAU - Annambhotla, Pallavi AU - Annambhotla P AUID- ORCID: 0000-0001-5564-8785 AD - Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. FAU - Lester, Keith AU - Lester K AUID- ORCID: 0009-0008-6469-3893 AD - Marsh Regional Blood Center, Kingsport, Tennessee, USA. FAU - Sukel-Hanlon, Steffanie AU - Sukel-Hanlon S AD - Marsh Regional Blood Center, Kingsport, Tennessee, USA. FAU - Galel, Susan A AU - Galel SA AUID- ORCID: 0009-0008-9791-6379 AD - Roche Diagnostics, USA. FAU - Williams, Seymour AU - Williams S AUID- ORCID: 0000-0003-2631-2216 AD - Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. FAU - Basavaraju, Sridhar V AU - Basavaraju SV AUID- ORCID: 0000-0002-8376-0499 AD - Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. FAU - Ridpath, Alison D AU - Ridpath AD AUID- ORCID: 0000-0002-4109-3464 AD - Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. FAU - Mace, Kimberly E AU - Mace KE AD - Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. FAU - Kracalik, Ian AU - Kracalik I AUID- ORCID: 0000-0003-3233-9466 AD - Division of Healthcare Quality Promotion, Centers for Disease Control and Prevention, Atlanta, Georgia, USA. LA - eng PT - Case Reports PT - Journal Article DEP - 20261029 PL - United States TA - Transfusion JT - Transfusion JID - 0417360 SB - IM OTO - NOTNLM OT - Plasmodium ovale OT - blood donation OT - malaria OT - near-miss OT - relapse OT - travel deferral EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:23 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/10/02 00:00 [revised] PHST- 2026/09/08 00:00 [received] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/30 01:23 [entrez] AID - 10.1111/trf.70452 [doi] PST - aheadofprint SO - Transfusion. 2026 Oct 29. doi: 10.1111/trf.70452. PMID- 42958640 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2050-4527 (Electronic) IS - 2050-4527 (Linking) VI - 14 IP - 10 DP - 2026 Oct TI - Analysis of Factors Associated With Interstitial Lung Disease in Treatment-Naive Rheumatoid Arthritis Patients and Development of a Classification Nomogram. LID - e70571 [pii] LID - 10.1002/iid3.70571 [doi] AB - BACKGROUND: Interstitial lung disease (ILD) is a serious extra-articular manifestation of rheumatoid arthritis (RA) and contributes substantially to morbidity and mortality. Early identification of RA-associated ILD (RA-ILD) remains challenging, and reliable biomarkers for risk stratification are lacking. OBJECTIVE: This study aimed to identify cytokine-related factors associated with RA-ILD and to develop an exploratory classification model for assessing the presence of ILD in patients with newly diagnosed, treatment-naive RA. METHODS: A total of 66 newly diagnosed, treatment-naive RA patients were enrolled, including individuals with and without ILD confirmed by high-resolution computed tomography (HRCT). All patients were disease-modifying antirheumatic drug (DMARD)-naive and had not received glucocorticoids or biologics prior to enrollment. Plasma IL-22 concentration was measured by ELISA; other cytokines (IL-2, IL-4, IL-6, IL-10, IL-12p70, IL-17A, IFN-gamma, and TNF-alpha) were quantified using a multiplex bead-based immunoassay. Clinical characteristics, laboratory parameters, and radiological findings were collected. Feature selection was performed using least absolute shrinkage and selection operator (LASSO) regression and Boruta algorithms, followed by multivariable logistic regression to identify independent factors associated with RA-ILD. A nomogram was constructed based on selected predictors, and its performance was evaluated using receiver operating characteristic analysis and cross-validation. RESULTS: Patients with RA-ILD exhibited distinct cytokine profiles compared with those without ILD. Multivariable analysis identified age, smoking, lower mean corpuscular hemoglobin concentration (MCHC), higher plasma TNF-alpha levels and lower plasma IL-22 levels as independent factors associated with RA-ILD. The nomogram exhibited good discriminative performance, with a fivefold cross-validated AUC of 0.851 (95% CI: 0.755-0.947); the apparent AUC in the full sample was 0.87. Internal validation indicated acceptable model stability. CONCLUSION: Age, smoking, MCHC, higher plasma TNF-alpha levels and lower plasma IL-22 levels were associated with the presence of ILD in newly diagnosed, treatment-naive RA patients. The proposed cytokine-based nomogram may serve as a hypothesis-generating tool for early identification of RA-ILD. Further validation in larger, independent cohorts is warranted before clinical application. CI - (c) 2026 The Author(s). Immunity, Inflammation and Disease published by John Wiley & Sons Ltd. FAU - Lu, Li AU - Lu L AD - Department of Rheumatology and Immunology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. FAU - Chen, Yongkang AU - Chen Y AD - Department of Rheumatology and Immunology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. FAU - Zhang, Ting AU - Zhang T AD - Department of Rheumatology and Immunology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. FAU - Cao, Lina AU - Cao L AD - Department of Rheumatology and Immunology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. FAU - Ma, Hua AU - Ma H AD - Department of Rheumatology and Immunology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. FAU - Song, Yuanyuan AU - Song Y AD - Department of Rheumatology and Immunology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. FAU - Zhou, Dongmei AU - Zhou D AUID- ORCID: 0000-0003-0163-8164 AD - Department of Rheumatology and Immunology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. LA - eng GR - 2021ZA17/Affiliated Hospital of Xuzhou Medical University/ PT - Journal Article PL - England TA - Immun Inflamm Dis JT - Immunity, inflammation and disease JID - 101635460 RN - 0 (Biomarkers) RN - 0 (Cytokines) RN - 0 (Interleukin-22) RN - 0 (Interleukins) SB - IM MH - Humans MH - *Arthritis, Rheumatoid/complications/blood MH - *Lung Diseases, Interstitial/diagnosis/blood/etiology MH - Female MH - Male MH - Middle Aged MH - *Nomograms MH - Biomarkers/blood MH - Cytokines/blood MH - Tomography, X-Ray Computed MH - Aged MH - Interleukin-22 MH - Risk Factors MH - Adult MH - Interleukins/blood MH - ROC Curve OTO - NOTNLM OT - IL-22 OT - TNF-alpha OT - cytokines OT - interstitial lung disease OT - nomogram OT - rheumatoid arthritis EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:23 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/09/08 00:00 [revised] PHST- 2025/12/27 00:00 [received] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/30 01:23 [entrez] AID - e70571 [pii] AID - 10.1002/iid3.70571 [doi] PST - ppublish SO - Immun Inflamm Dis. 2026 Oct;14(10):e70571. doi: 10.1002/iid3.70571. PMID- 42958637 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1738-8872 (Electronic) IS - 1017-7825 (Linking) VI - 36 DP - 2026 Oct 13 TI - Elevated Level of Fannyhessea vaginae Correlated with Implantation Outcomes in Women undergoing in vitro fertilization Treatment. PG - e2606022 LID - 10.4014/jmb.2606.06022 [doi] AB - The vaginal microbiome plays a significant role in reproductive health. While maintaining a balanced vaginal microbiome is crucial for successful in vitro fertilization (IVF) outcomes, the specific bacteria associated with adverse outcomes may vary by region and are largely unknown. This study aims to investigate the bacterial profiles and their influence on embryo implantation, thereby supporting the development of microbiome-based therapies. One hundred women undergoing IVF provided vaginal swabs at three stages: oocyte retrieval, embryo transfer, and hCG pregnancy testing. An in-house 16S rRNA gene amplicon sequencing and bioinformatics pipeline was used to profile the vaginal microbiota. Bacterial communities were constructed, and the transitions across IVF stages were analyzed. We also used custom quantitative PCR assays to validate NGS results and provide species-level resolution for selected bacterial species. Unlike other regions where Lactobacillus crispatus predominates, we found that Lactobacillus iners is the main vaginal microbiota across all IVF stages in Taiwan. Nevertheless, significant shifts in bacterial community types were correlated with hormonal changes during IVF treatment. Importantly, while Lactobacillus levels didn't predict pregnancy success, higher levels of bacteria associated with bacterial vaginosis, particularly Fannyhessea vaginae, significantly correlated with implantation failure and early embryonic loss. The strong association between elevated F. vaginae levels and adverse implantation rate in Taiwanese women highlights the potential benefit of incorporating routine screening for this pathogen. Personalized microbiome interventions targeting F. vaginae may improve embryo implantation for women undergoing assisted reproductive technologies. FAU - Paramita, Liya AU - Paramita L AD - Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan. AD - Center for Genomic Medicine, Research and Service Headquarter, National Cheng Kung University, Tainan, Taiwan. FAU - Teng, Ching-Hao AU - Teng CH AD - Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan. FAU - Ma, Mi-Chia AU - Ma MC AD - Department of Statistics, National Cheng Kung University, Tainan, Taiwan. FAU - Hashimoto, Masayuki AU - Hashimoto M AD - Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan. FAU - Tang, Yen-An AU - Tang YA AD - Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan. AD - Center for Genomic Medicine, Research and Service Headquarter, National Cheng Kung University, Tainan, Taiwan. FAU - Pan, Hsien-An AU - Pan HA AD - An-An Women and Children Clinic, Tainan, Taiwan. AD - Department of Obstetrics and Gynecology, College of Medicine, National Cheng Kung University, Tainan, Taiwan. FAU - Sun, H Sunny AU - Sun HS AD - Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan. AD - Center for Genomic Medicine, Research and Service Headquarter, National Cheng Kung University, Tainan, Taiwan. LA - eng PT - Journal Article DEP - 20261013 PL - Korea (South) TA - J Microbiol Biotechnol JT - Journal of microbiology and biotechnology JID - 9431852 RN - 0 (RNA, Ribosomal, 16S) RN - Lactobacillus iners SB - IM MH - Female MH - Humans MH - *Fertilization in Vitro MH - *Vagina/microbiology MH - *Embryo Implantation MH - Pregnancy MH - RNA, Ribosomal, 16S/genetics MH - *Microbiota/genetics MH - Adult MH - Embryo Transfer MH - Taiwan MH - Bacteria/classification/genetics/isolation & purification MH - Lactobacillus/isolation & purification/genetics MH - Vaginosis, Bacterial/microbiology OTO - NOTNLM OT - Embryonic loss OT - Fannyhessea vaginae OT - Implantation failure OT - Infertility OT - NGS OT - Vaginal microbiota EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:23 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/06/16 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/30 01:23 [entrez] AID - jmb.2606.06022 [pii] AID - 10.4014/jmb.2606.06022 [doi] PST - epublish SO - J Microbiol Biotechnol. 2026 Oct 13;36:e2606022. doi: 10.4014/jmb.2606.06022. PMID- 42958635 OWN - NLM STAT- Publisher LR - 20261030 IS - 1708-8240 (Electronic) IS - 1496-4155 (Linking) DP - 2026 Oct 29 TI - Substrate-Driven Adhesive Strategies for Durable Bonding to Coronal and Radicular Dentin Following Endodontic Procedures: A Systematic Review of In Vitro Evidence. LID - 10.1111/jerd.70288 [doi] AB - OBJECTIVES: To evaluate substrate-directed strategies affecting the durability of adhesive bonding to coronal and radicular dentin following endodontic procedures based on in vitro evidence. MATERIALS AND METHODS: A systematic search (PubMed, Scopus, Web of Science, EMBASE) identified in vitro studies assessing bond strength to human permanent coronal or radicular dentin following documented endodontic intervention. Eligible studies evaluated radicular dentin for fiber-post bonding or coronal/pulp-chamber dentin for direct restorative bonding under artificial aging conditions. Data on dentin pretreatment, adhesive systems, resin cements, and bond strength outcomes were extracted. Risk of bias was evaluated using ROBDEMat. Due to methodological heterogeneity, a narrative synthesis was performed. RESULTS: Twenty-four studies were included (21 fiber post; 3 coronal dentin). Risk of bias was low in 42%, moderate in 54%, and high in 4% of studies. In radicular dentin, chlorhexidine (CHX), ethanol wet bonding, EDTA conditioning, and collagen cross-linkers (carbodiimide (EDC) and dimethyl sulfoxide (DMSO)) generally improved aged bond durability, particularly in cervical regions. Ultrasonic and laser-assisted activation enhanced smear layer removal and interfacial adaptation. Resin cement performance varied with substrate condition, with 10-MDP-containing systems showing superior stability. Limited coronal/pulp-chamber evidence suggested potential benefits of short-term CHX and mild chelating/acidic pretreatments, with more consistent aging performance observed for two-step self-etch approaches in selected experimental conditions. CONCLUSIONS: Bond durability following endodontic procedures is strongly substrate dependent. Stable adhesion is promoted by collagen preservation, optimized smear layer management, and chemically interactive adhesive systems. However, substantial heterogeneity among in vitro studies, together with the exclusively in vitro nature of the evidence, limits direct comparability and precludes clinical standardization. CLINICAL SIGNIFICANCE: Substrate-directed pretreatment strategies, when combined with appropriate adhesive selection, may enhance the longevity of bonded restorations following endodontic procedures by reducing degradation at the resin-dentin interface. CI - (c) 2026 Wiley Periodicals LLC. FAU - Holiel, Ahmed A AU - Holiel AA AUID- ORCID: 0000-0002-3907-7879 AD - Department of Conservative Dentistry, Faculty of Dentistry, Alexandria University, Alexandria, Egypt. AD - Department of Restorative Sciences, Faculty of Dentistry, Beirut Arab University, Beirut, Lebanon. FAU - Nakouzi, Mounir M Al AU - Nakouzi MMA AUID- ORCID: 0009-0003-8084-5854 AD - Department of Restorative Sciences, Faculty of Dentistry, Beirut Arab University, Beirut, Lebanon. FAU - Cuevas-Suarez, Carlos Enrique AU - Cuevas-Suarez CE AUID- ORCID: 0000-0002-2759-8984 AD - Dental Materials Laboratory, Academic Area of Dentistry, Autonomous University of Hidalgo State, San Agustin Tlaxiaca, Mexico. AD - Dental Materials and Biomaterials Laboratory Faculty of Stomatology, Meritorious Autonomous University of Puebla, Puebla, Mexico. FAU - Breschi, Lorenzo AU - Breschi L AUID- ORCID: 0000-0001-7621-226X AD - Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy. FAU - Maravic, Tatjana AU - Maravic T AUID- ORCID: 0000-0003-1335-2817 AD - Department of Biomedical and Neuromotor Sciences, University of Bologna, Bologna, Italy. FAU - Bourgi, Rim AU - Bourgi R AUID- ORCID: 0000-0002-8003-2664 AD - Department of Restorative and Esthetic Dentistry, Faculty of Dental Medicine, Saint-Joseph University of Beirut, Beirut, Lebanon. AD - Department of Biomaterials and Bioengineering, INSERM UMR_S 1121, University of Strasbourg, Strasbourg, France. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - J Esthet Restor Dent JT - Journal of esthetic and restorative dentistry : official publication of the American Academy of Esthetic Dentistry ... [et al.] JID - 101096515 SB - IM OTO - NOTNLM OT - adhesive systems OT - chlorhexidine OT - coronal dentin OT - dentin bonding OT - endodontic procedures OT - fiber posts OT - radicular dentin OT - resin cements OT - surface pretreatment EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 01:18 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/09/11 00:00 [revised] PHST- 2026/05/17 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/30 01:18 [entrez] AID - 10.1111/jerd.70288 [doi] PST - aheadofprint SO - J Esthet Restor Dent. 2026 Oct 29. doi: 10.1111/jerd.70288. PMID- 42958633 OWN - NLM STAT- Publisher LR - 20261030 IS - 1526-4998 (Electronic) IS - 1526-498X (Linking) DP - 2026 Oct 29 TI - Silencing RpadOrco disrupts host seeking, semiochemical avoidance and reproductive fitness in Rhopalosiphum padi. LID - 10.1002/ps.71449 [doi] AB - BACKGROUND: The odorant receptor co-receptor (Orco) is an important component of the insect olfactory system. However, the biological functions of Orco in the bird cherry-oat aphid, Rhopalosiphum padi, remain unclear, particularly its roles in olfactory perception, host-plant selection and aphid fitness. RESULTS: RpadOrco was predominantly expressed in the antennae. RNA interference-mediated knockdown of RpadOrco significantly reduced electroantennogram responses to 11 behaviorally relevant odorants, including plant volatiles, the repellent methyl salicylate (MeSA) and the alarm pheromone (E)-beta-farnesene (EBF). In Y-tube olfactometer assays, RpadOrco-silenced aphids no longer showed significant orientation toward wheat and exhibited attenuated avoidance responses to MeSA and EBF. Silencing RpadOrco also increased the proportion of aphids that made no choice in the Y-tube olfactometer assays and significantly reduced body weight and fecundity in the F(0) generation, without affecting survival. However, no significant changes were detected in developmental duration, longevity or life-table parameters in the F(1) generation. CONCLUSION: RpadOrco plays an important role in host-plant orientation, semiochemical perception and reproductive fitness in R. padi. Its simultaneous effects on olfactory behavior and fecundity identify RpadOrco as a potential molecular target for developing behavior-disrupting and RNAi-based approaches to cereal aphid management. (c) 2026 Society of Chemical Industry. CI - (c) 2026 Society of Chemical Industry. FAU - Zhang, Ting AU - Zhang T AD - College of Plant Protection, Jilin Agricultural University, Jilin, China. FAU - Liu, Hanyu AU - Liu H AD - College of Plant Protection, Jilin Agricultural University, Jilin, China. FAU - Sui, Mingxuan AU - Sui M AD - College of Plant Protection, Jilin Agricultural University, Jilin, China. FAU - Xu, Yingyue AU - Xu Y AD - College of Plant Protection, Jilin Agricultural University, Jilin, China. FAU - Wang, Yan AU - Wang Y AD - College of Plant Protection, Jilin Agricultural University, Jilin, China. FAU - Wang, Shengdi AU - Wang S AD - College of Plant Protection, Jilin Agricultural University, Jilin, China. FAU - Zhao, Yunpeng AU - Zhao Y AD - College of Plant Protection, Jilin Agricultural University, Jilin, China. FAU - Jia, Ze AU - Jia Z AD - College of Plant Protection, Jilin Agricultural University, Jilin, China. FAU - Liu, Shuai AU - Liu S AUID- ORCID: 0009-0009-8472-720X AD - College of Plant Protection, Jilin Agricultural University, Jilin, China. FAU - Yuan, Haibin AU - Yuan H AD - College of Plant Protection, Jilin Agricultural University, Jilin, China. LA - eng GR - Jilin Province Youth Science and Technology Talent Support Project, China (QT202307)/ GR - U23A6001/National Natural Science Foundation of China/ GR - 32202311/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261029 PL - England TA - Pest Manag Sci JT - Pest management science JID - 100898744 SB - IM OTO - NOTNLM OT - Orco OT - RNA interference OT - Rhopalosiphum padi OT - fecundity OT - olfactory perception OT - potential molecular target EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 01:18 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/10 00:00 [revised] PHST- 2026/07/26 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/30 01:18 [entrez] AID - 10.1002/ps.71449 [doi] PST - aheadofprint SO - Pest Manag Sci. 2026 Oct 29. doi: 10.1002/ps.71449. PMID- 42958632 OWN - NLM STAT- Publisher LR - 20261030 IS - 1557-7732 (Electronic) IS - 1080-7683 (Linking) DP - 2026 Oct 29 TI - Emerging Ocular-Surface Antimicrobial Delivery Strategies for Infectious Keratitis. PG - 10807683261501328 LID - 10.1177/10807683261501328 [doi] AB - PURPOSE: Infectious keratitis remains a leading cause of corneal blindness worldwide despite effective antimicrobial agents. Conventional topical therapy is limited by rapid precorneal clearance, restricted corneal penetration, and inadequate stromal drug exposure, resulting in subtherapeutic drug concentrations. These pharmacokinetic barriers are particularly important in fungal, protozoal, deep stromal, and antimicrobial-resistant keratitis, where prolonged or targeted exposure is essential for successful treatment. This review evaluates emerging ocular antimicrobial delivery strategies through a pharmacokinetic framework, emphasizing the relationship among delivery platform, depth of corneal involvement, pathogen-specific requirements, and clinical feasibility. METHODS: We searched PubMed and Scopus for literature published from 2017 through August 31, 2026, including preclinical studies, clinical trials, and regulatory records. Platforms covered include polymeric nanoparticles, solid lipid nanoparticles, nanostructured lipid carriers, dendrimers, hybrid nanocarriers, mucoadhesive formulations, hydrogels, drug-eluting contact lenses, ocular inserts, microneedles, intrastromal delivery, photoactivated chromophore for keratitis corneal cross-linking (PACK-CXL), and amniotic membrane. Quantitative pharmacokinetic outcomes were compared with those of conventional formulations. RESULTS: Each platform addresses a distinct pharmacokinetic challenges, so platforms function as complementary options. Quantitative pharmacokinetic data are compared to assess improvements in ocular residence, corneal exposure, and sustained drug delivery relative to conventional formulations. A decision-tree framework is proposed to guide platform selection by drug lipophilicity, corneal permeability, and dosing requirements. Newer systemic anti-infective agents with potential for ocular reformulation are also identified. Hybrid systems increasingly target multiple limitations simultaneously, but most evidence remains preclinical, and human corneal pharmacokinetic data are limited. CONCLUSIONS: Future studies should prioritize direct comparisons of novel platforms with standard therapy and establish whether improved delivery maintains concentrations above pathogen-specific inhibitory thresholds and translates into superior clinical outcomes. FAU - Stark, Rain AU - Stark R AD - Department of Biomedical Sciences, Heatherington College of Osteopathic Medicine, Western University of Health Sciences, Lebanon, Oregon, USA. FAU - Tice, Andrew AU - Tice A AD - Department of Biomedical Sciences, Heatherington College of Osteopathic Medicine, Western University of Health Sciences, Lebanon, Oregon, USA. FAU - Tiwari, Rakesh Kumar AU - Tiwari RK AUID- ORCID: 0000-0003-3701-4548 AD - Department of Biomedical Sciences, Heatherington College of Osteopathic Medicine, Western University of Health Sciences, Lebanon, Oregon, USA. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - United States TA - J Ocul Pharmacol Ther JT - Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics JID - 9511091 SB - IM OTO - NOTNLM OT - amniotic membrane OT - antimicrobial therapy OT - hydrogels OT - infectious keratitis OT - nanocarriers OT - ocular drug delivery EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 01:18 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/30 01:18 [entrez] AID - 10.1177/10807683261501328 [doi] PST - aheadofprint SO - J Ocul Pharmacol Ther. 2026 Oct 29:10807683261501328. doi: 10.1177/10807683261501328. PMID- 42958629 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2045-7634 (Electronic) IS - 2045-7634 (Linking) VI - 15 IP - 11 DP - 2026 Nov TI - Further Insights Into the Mechanisms of Lymph Node Metastasis. LID - e72404 [pii] LID - 10.1002/cam4.72404 [doi] AB - Metastasis of malignant tumors arising from epithelial organs to sentinel lymph nodes has important implications for prognosis, staging, and therapeutic decision-making. Recent insights into anatomic pathways, pathogenesis, molecular interactions, and genetic factors have encouraged scientists and clinicians to develop drugs that target mechanisms of lymph node metastasis. We summarize important aspects of the vast literature that pertains to these mechanisms, focusing on five phases that implicate invasion through the epithelial basement membrane and tumor microenvironment, into initial lymphatics, and, after flow through lymphatic trunks, into the sentinel lymph node. Valuable information regarding tumor cell-directed motility, lymphangiogenesis, host cells that enhance metastasis, immune responses to tumor antigens, and tumor-induced changes in sentinel lymph node(s) provides a promising avenue for the continued development of new therapeutic approaches to prevent and treat metastasis. This article reviews these aspects by synthesizing current scientific and clinical advances and translating them for medical professionals. CI - (c) 2026 The Author(s). Cancer Medicine published by John Wiley & Sons Ltd. FAU - Nathanson, S David AU - Nathanson SD AUID- ORCID: 0000-0002-5254-9789 AD - Department of Surgery, Henry Ford Cancer Institute, Henry Ford Health, Detroit, Michigan, USA. FAU - O'Melia, Meghan J AU - O'Melia MJ AUID- ORCID: 0000-0003-2223-1849 AD - Edwin L. Steele Laboratories for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital Cancer Center, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA. FAU - Rosso, Kelly C AU - Rosso KC AD - Banner MD Anderson Cancer Center, Sun City West, Arizona, USA. FAU - Wood, Ian S AU - Wood IS AD - Department of Surgery, Henry Ford Cancer Institute, Henry Ford Health, Detroit, Michigan, USA. AD - Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, British Columbia, Canada. FAU - Lall, Drishti AU - Lall D AD - Department of Surgery, Henry Ford Cancer Institute, Henry Ford Health, Detroit, Michigan, USA. FAU - Bui, Jenny T AU - Bui JT AD - Department of Surgery, Henry Ford Cancer Institute, Henry Ford Health, Detroit, Michigan, USA. LA - eng GR - J98004/Henry Ford Health/ PT - Journal Article PT - Review PL - United States TA - Cancer Med JT - Cancer medicine JID - 101595310 SB - IM MH - Humans MH - *Lymphatic Metastasis/pathology MH - Tumor Microenvironment MH - Lymphangiogenesis MH - Animals MH - Sentinel Lymph Node/pathology MH - *Neoplasms/pathology MH - Cell Movement MH - Lymph Nodes/pathology OTO - NOTNLM OT - invasion OT - lymphatics OT - metastasis OT - sentinel lymph node OT - tumor microenvironment EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:18 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/06 00:00 [revised] PHST- 2026/06/02 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/30 01:18 [entrez] AID - e72404 [pii] AID - 10.1002/cam4.72404 [doi] PST - ppublish SO - Cancer Med. 2026 Nov;15(11):e72404. doi: 10.1002/cam4.72404. PMID- 42958625 OWN - NLM STAT- Publisher LR - 20261030 IS - 1558-9455 (Electronic) IS - 1558-9447 (Linking) DP - 2026 Oct 29 TI - Surgical Management of Unstable Distal Ulnar Fractures With Concomitant Distal Radius Fractures: A Systematic Review. PG - 15589447261496464 LID - 10.1177/15589447261496464 [doi] AB - Unstable distal ulnar fractures (DUFs) often accompany distal radius fractures (DRFs) and can cause pain, functional impairment, and long-term instability if not managed appropriately. Given the lack of consensus regarding the optimal surgical technique, we conducted a systematic review to compare outcomes of different surgical treatments for unstable DUFs associated with DRFs. A systematic review was conducted in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines from inception to July 16, 2025. English studies involving adult patients with surgically treated unstable DUFs concomitant with DRFs were included (ulnar styloid-only fractures excluded). Two independent reviewers screened studies and extracted data on demographics, fracture characteristics, treatment methods, and outcomes. The outcomes were postoperative range of motion, pain, complications, and reoperation rates. Twenty-four studies (608 patients) met inclusion criteria. The mean age was 65 years, 80% were women, and the mean follow-up was 22 months. Surgical techniques included plate fixation (58%), pin and wire fixation (22%), Darrach resection (19%), and screws only (2%). Reoperation occurred in 39 patients (5.5%). Time to union, reported in four studies, averaged 11 weeks. Meta-analyses showed no significant differences among techniques in treatment outcomes. Overall, no fixation technique demonstrated superiority in outcomes, suggesting that multiple surgical strategies can yield satisfactory outcomes when appropriately selected. FAU - den Hengst, Stella AU - den Hengst S AUID- ORCID: 0009-0006-7164-5430 AD - Massachusetts General Hospital, Boston, USA. AD - Erasmus University Medical Centre Rotterdam, The Netherlands. AD - Yawkey Center for Outpatient Care, Boston, MA, USA. FAU - Ghaderi, Navid AU - Ghaderi N AD - University of Toronto, ON, Canada. FAU - Adams, Alex J AU - Adams AJ AUID- ORCID: 0000-0002-7911-4021 AD - Massachusetts General Hospital, Boston, USA. FAU - Wijffels, Mathieu M E AU - Wijffels MME AD - Erasmus University Medical Centre Rotterdam, The Netherlands. FAU - Bhashyam, Abhiram R AU - Bhashyam AR AD - Massachusetts General Hospital, Boston, USA. AD - Harvard Medical School, Boston, MA, USA. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - United States TA - Hand (N Y) JT - Hand (New York, N.Y.) JID - 101264149 SB - IM OTO - NOTNLM OT - distal radius fracture OT - distal ulnar fracture OT - open reduction and internal fixation (ORIF) OT - surgical treatment OT - systematic review EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 01:17 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/30 01:17 [entrez] AID - 10.1177/15589447261496464 [doi] PST - aheadofprint SO - Hand (N Y). 2026 Oct 29:15589447261496464. doi: 10.1177/15589447261496464. PMID- 42958621 OWN - NLM STAT- Publisher LR - 20261030 IS - 1349-7006 (Electronic) IS - 1347-9032 (Linking) DP - 2026 Oct 29 TI - ADAM19 Promotes EMT-Related Changes and Serves as a Prognostic Marker in Circulating Tumor Cells of Renal Cell Carcinoma. LID - 10.1111/cas.70571 [doi] AB - Detection of circulating tumor cells (CTCs), particularly characterization of their epithelial-mesenchymal transition (EMT) phenotypes, may improve prognostic accuracy for malignancies. Identification of novel biomarkers for CTC classification may improve the characterization of tumor progression and provide potential therapeutic insights for renal cell carcinoma (RCC). Herein, we identified that a disintegrin and metalloproteinase domain-containing protein 19 (ADAM19) was associated with EMT phenotypes of CTCs. In vitro functional experiments, Western blotting, and RNA sequencing demonstrated that ADAM19 promoted RCC malignant progression and EMT-related changes and was associated with NOTCH and NF-kappaB signaling pathways. Our findings further suggested potential bidirectional association between ADAM19 and these signaling pathways. CTCs isolated from xenograft mouse models exhibited enhanced proliferative, migratory, and invasive abilities, accompanied by increased ADAM19 expression compared with parental tumor cells. Clinical CTC analysis further revealed that ADAM19-Low-CTCs were associated with unfavorable clinical outcomes in RCC patients. Notably, higher time-updated ADAM19-Low-CTC counts were identified as an independent risk factor for progression-free survival (HR = 1.243, 95% CI: 1.006-1.536). CI - (c) 2026 The Author(s). Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. FAU - He, Minxin AU - He M AD - Department of Urology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. FAU - Li, Mingrui AU - Li M AD - Department of Urology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. FAU - Ma, Yifei AU - Ma Y AD - Department of Urology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. FAU - Chen, Zhenzhuo AU - Chen Z AD - Department of Urology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. FAU - Guo, Lingyu AU - Guo L AD - Department of Urology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. FAU - Wang, Xiangyu AU - Wang X AD - Department of Urology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. FAU - Wang, Wenjing AU - Wang W AD - Department of Pathology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. FAU - Chong, Tie AU - Chong T AUID- ORCID: 0000-0002-2035-6544 AD - Department of Urology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. FAU - Wang, Zhenlong AU - Wang Z AUID- ORCID: 0009-0002-9203-7777 AD - Department of Urology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. LA - eng GR - 320.6750.2025-16-19/Wu Jieping Medical Foundation Scientific Research Special Project/ GR - z014/Key Project of IIT Clinical Research Fund, The Second Affiliated Hospital of Xi‧an Jiaotong University/ PT - Journal Article DEP - 20261029 PL - England TA - Cancer Sci JT - Cancer science JID - 101168776 SB - IM OTO - NOTNLM OT - ADAM19 OT - circulating tumor cells OT - epithelial-mesenchymal transition OT - prognostic marker OT - renal cell carcinoma EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 01:17 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/09/16 00:00 [revised] PHST- 2026/06/06 00:00 [received] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/30 01:17 [entrez] AID - 10.1111/cas.70571 [doi] PST - aheadofprint SO - Cancer Sci. 2026 Oct 29. doi: 10.1111/cas.70571. PMID- 42958617 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1526-9914 (Electronic) IS - 1526-9914 (Linking) VI - 27 IP - 11 DP - 2026 Nov TI - A multicenter end-to-end postal audit of skin markerless surface-guided radiation therapy. LID - e70893 [pii] LID - 10.1002/acm2.70893 [doi] AB - BACKGROUND: Surface-guided radiation therapy (SGRT) enables skin markerless positioning, but its accuracy may depend on the reference surface, hardware configuration, commissioning, and institutional workflow. Two reference surfaces are commonly used: a DICOM reference generated from planning CT images and an SGRT reference acquired in the treatment room. Multicenter end-to-end data across commercial systems remain limited. PURPOSE: To evaluate static irradiation accuracy and setup time with two reference surfaces in a nationwide multicenter skin markerless SGRT postal audit. METHODS: A prospective end-to-end postal audit was conducted from October 2023 to July 2025 at 40 Japanese institutions using five commercial SGRT systems (AlignRT, Catalyst, ExacTrac Dynamic, IDENTIFY, and VOXELAN), eight per system. Each institution received a common phantom package: a breast-shaped surface phantom, two cubic phantoms containing gold markers, and radiochromic films. Markerless setup and irradiation were performed with both the DICOM and SGRT references; ExacTrac Dynamic institutions performed the DICOM reference setup only. Static irradiation accuracy was the difference between the film-derived radiation center and the intended center in the DICOM RT Plan; the primary metric was the three-dimensional translational vector error (3D vector error). Paired comparisons used the Wilcoxon signed-rank test, and multivariable linear regression evaluated factors associated with both endpoints. RESULTS: Critical setup errors exceeding 5 mm occurred at four institutions initially (institution-level error rate, 10.0%; three with the DICOM and one with the SGRT reference); one DICOM reference setup was not remeasured and remained out of tolerance, and was excluded as an outlier, leaving 39 DICOM and 31 SGRT reference setups for analysis. Excluding ExacTrac Dynamic, the DICOM reference showed a significantly larger 3D vector error than the SGRT reference (1.54 +/- 0.91 vs. 1.20 +/- 0.61 mm, p = 0.043). Setup time did not differ between references (86.19 +/- 43.57 vs. 81.61 +/- 37.09 s, p = 0.804). Axis-specifically, the difference between references was confined to the anterior-posterior direction (mean absolute difference 0.36 mm, p = 0.002), whereas a caudal superior-inferior offset was present with both (-0.50 +/- 1.29 and -0.37 +/- 0.87 mm; both p < 0.01) and did not differ between them (p = 0.854). The 1-camera condition was independently associated with a larger 3D vector error than the 3-camera condition (beta = 0.90 mm; 95% CI, 0.17-1.63 mm; p = 0.017); no factor was associated with setup time, which showed no correlation with 3D vector error. CONCLUSIONS: Skin markerless SGRT achieved millimeter-level static irradiation accuracy but carried a non-negligible risk of critical setup errors. The difference between the two reference workflows was confined to the anterior-posterior direction, whereas a superior-inferior offset common to both indicates a residual uncertainty in the audit chain itself. Reference surface selection and camera configuration influenced accuracy, whereas setup time was not a reliable surrogate. These findings highlight the importance of commissioning, standardized reference surface acquisition, routine QA, and independent third-party audits. CI - (c) 2026 The Author(s). Journal of Applied Clinical Medical Physics published by Wiley Periodicals, LLC on behalf of The American Association of Physicists in Medicine. FAU - Saito, Masahide AU - Saito M AUID- ORCID: 0000-0002-8636-4045 AD - Department of Therapeutic Radiology, University of Yamanashi, Yamanashi, Japan. FAU - Yamauchi, Ryohei AU - Yamauchi R AUID- ORCID: 0000-0001-7719-143X AD - Department of Radiation Oncology, St. Luke's International Hospital, Tokyo, Japan. FAU - Kojima, Hironori AU - Kojima H AUID- ORCID: 0000-0001-9605-2742 AD - Department of Radiology, Kanazawa University Hospital, Ishikawa, Japan. FAU - Shimohigashi, Yoshinobu AU - Shimohigashi Y AUID- ORCID: 0000-0003-2948-0733 AD - Department of Radiological Technology, Kumamoto University Hospital. FAU - Ono, Takeshi AU - Ono T AUID- ORCID: 0000-0002-5946-389X AD - Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan. FAU - Oku, Yoshifumi AU - Oku Y AD - Department of Radiology, Kagoshima University Hospital, Kagoshima, Japan. FAU - Kumagai, Shinobu AU - Kumagai S AD - Department of Radiology, Teikyo University Hospital, Tokyo, Japan. FAU - Tohyama, Naoki AU - Tohyama N AD - Department of Radiological Sciences, Komazawa University, Tokyo, Japan. FAU - Kurooka, Masahiko AU - Kurooka M AD - Department of Radiation Therapy, Tokyo Medical University Hospital, Tokyo, Japan. FAU - Saito, Eriko AU - Saito E AD - Department of Radiation Oncology, Chiba Tokushukai Hospital, Chiba, Japan. FAU - Sakamoto, Masataka AU - Sakamoto M AD - Department of Radiology, Hamamatsu University School of Medicine, Shizuoka, Japan. FAU - Ueshima, Yusuke AU - Ueshima Y AUID- ORCID: 0009-0006-6256-3249 AD - Regional Creative Education Center, Hamamatsu University School of Medicine, Shizuoka, Japan. FAU - Kanai, Takayuki AU - Kanai T AD - Department of Radiation Oncology, Tokyo Women's Medical University, Tokyo, Japan. FAU - Hayashi, Naoki AU - Hayashi N AUID- ORCID: 0000-0002-1720-3651 AD - Division of Medical Physics, School of Medical Sciences, Fujita Health University, Aichi, Japan. FAU - Saito, Tatsunori AU - Saito T AD - Department of Radiology, Seirei Hamamatsu General Hospital, Shizuoka, Japan. FAU - Kitagawa, Tomoki AU - Kitagawa T AD - Department of Radiation Oncology, Aichi Cancer Center, Aichi, Japan. FAU - Sasaki, Makoto AU - Sasaki M AD - Division of clinical radiology service, Kyoto University Hospital, Kyoto, Japan. FAU - Katayama, Hiroki AU - Katayama H AUID- ORCID: 0000-0002-5836-0004 AD - Division of Clinical Radiology, Department of Medical Technology, Kagawa University Hospital, Kagawa, Japan. FAU - Suzuki, Hidekazu AU - Suzuki H AUID- ORCID: 0009-0007-4412-0409 AD - Department of Radiological Technology, Faculty of Medical Technology, Teikyo University, Tokyo, Japan. FAU - Kurokawa, Chie AU - Kurokawa C AD - Faculty of Health Science, Juntendo University, Tokyo, Japan. FAU - Onishi, Hiroshi AU - Onishi H AD - Department of Therapeutic Radiology, University of Yamanashi, Yamanashi, Japan. LA - eng GR - Grant-in-aid for research on radiation oncology JASTRO 2024-2025/ PT - Journal Article PT - Multicenter Study PL - United States TA - J Appl Clin Med Phys JT - Journal of applied clinical medical physics JID - 101089176 SB - IM MH - Humans MH - *Phantoms, Imaging MH - *Radiotherapy Planning, Computer-Assisted/methods/standards MH - Radiotherapy Dosage MH - *Radiotherapy, Image-Guided/methods/standards MH - Prospective Studies MH - Radiotherapy, Intensity-Modulated/methods MH - *Quality Assurance, Health Care/standards MH - *Neoplasms/radiotherapy OTO - NOTNLM OT - breast cancer OT - postal audit OT - surface-guided radiation therapy EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:17 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/19 00:00 [revised] PHST- 2026/06/26 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/30 01:17 [entrez] AID - e70893 [pii] AID - 10.1002/acm2.70893 [doi] PST - ppublish SO - J Appl Clin Med Phys. 2026 Nov;27(11):e70893. doi: 10.1002/acm2.70893. PMID- 42958610 OWN - NLM STAT- Publisher LR - 20261030 IS - 1938-2707 (Electronic) IS - 0009-9228 (Linking) DP - 2026 Oct 29 TI - Factors Associated With Invasive Mechanical Ventilation in Pediatric Drowning Patients Admitted to Intensive Care: A 14-Year Retrospective Study. PG - 99228261493363 LID - 10.1177/00099228261493363 [doi] AB - Drowning is a major cause of morbidity and mortality in children, primarily due to respiratory and neurological injury. This study aimed to describe the epidemiological, clinical, and outcome characteristics of pediatric drowning and identify factors associated with invasive mechanical ventilation. We conducted a 14-year retrospective descriptive and analytical study including all children admitted to a pediatric intensive care unit for drowning. Thirty-one patients were included, with a median age of 30 months, and 61% were male. The median immersion time was 5 minutes, and 13% of children were submerged for more than 10 minutes. Respiratory distress occurred in 71% of cases, while 41.9% required invasive mechanical ventilation. Prolonged immersion, SpO(2) <90%, respiratory distress, neurological impairment, advanced clinical stage, and biological abnormalities were associated with mechanical ventilation. Mortality was 9.7% and was higher among ventilated patients. Early recognition and appropriate respiratory support may improve outcomes. FAU - Gargouri, Marouen AU - Gargouri M AUID- ORCID: 0009-0000-7237-494X AD - Department of Family Medicine, Faculty of Medicine, University of Sfax, Tunisia. FAU - Elleuch, Amal AU - Elleuch A AUID- ORCID: 0000-0002-0897-8930 AD - Department of Pediatric Reanimation, Hedi Chaker University Hospital, University of Sfax, Tunisia. AD - Faculty of Medicine of Sfax, University of Sfax, Tunisia. FAU - Ben Halima, Asma AU - Ben Halima A AD - Department of Pediatric Reanimation, Hedi Chaker University Hospital, University of Sfax, Tunisia. AD - Faculty of Medicine of Sfax, University of Sfax, Tunisia. FAU - Feki, Yessine AU - Feki Y AD - Department of Family Medicine, Faculty of Medicine, University of Sfax, Tunisia. FAU - Elleuch, Imen AU - Elleuch I AD - Department of Pediatric Reanimation, Hedi Chaker University Hospital, University of Sfax, Tunisia. AD - Faculty of Medicine of Sfax, University of Sfax, Tunisia. FAU - Safi, Faiza AU - Safi F AD - Department of Pediatric Reanimation, Hedi Chaker University Hospital, University of Sfax, Tunisia. AD - Faculty of Medicine of Sfax, University of Sfax, Tunisia. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Clin Pediatr (Phila) JT - Clinical pediatrics JID - 0372606 SB - IM OTO - NOTNLM OT - acute respiratory failure OT - associated factors OT - children OT - drowning OT - mechanical ventilation OT - pediatric intensive care OT - prognosis EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 01:17 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/30 01:17 [entrez] AID - 10.1177/00099228261493363 [doi] PST - aheadofprint SO - Clin Pediatr (Phila). 2026 Oct 29:99228261493363. doi: 10.1177/00099228261493363. PMID- 42958597 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1751-0147 (Electronic) IS - 0044-605X (Linking) VI - 68 IP - 1 DP - 2026 Oct 29 TI - Curcumin extract addition to bull semen does not adversely affect in vitro fertilization. LID - 47 [pii] LID - 10.1186/s13028-026-00890-5 [doi] AB - BACKGROUND: Curcumin extract has demonstrated antioxidant and antibacterial properties in bull semen samples at a concentration of 5%. Nevertheless, its impact on fertilization outcomes remains largely unexplored. This study aimed to evaluate if curcumin extract added to bull semen had an effect on in vitro fertilization (IVF) results. Bovine ovaries were collected from an abattoir, cumulus-oocyte complexes (COCs) were aspirated, graded, and randomly assigned to treatment groups before maturation. The COCs were matured for 22 hours and fertilized using semen from one bull with verified fertility. Semen samples were thawed and treated with 5% curcumin extract for 30 minutes at room temperature, and IVF was conducted at a reduced sperm concentration (0.5 x 10(6)/mL) to better assess treatment effects. Cleavage was evaluated, 44 hours post-fertilization, and embryo development was assessed on day 8. Sperm quality parameters were evaluated using Computer-Assisted Sperm Analysis for motility and kinematics, and Flow Cytometry for viability markers, including membrane integrity, mitochondrial membrane potential (MMP), and chromatin integrity. RESULTS: No statistically significant differences were observed between groups for any IVF outcomes. A trend toward significance was observed for total motility and immotile spermatozoa, with higher motility and lower immotility in the curcumin-treated group compared to controls. CONCLUSION: The results demonstrated that curcumin treatment does not seem to have an effect on in vitro fertilization (IVF) outcomes since no statistically significant differences were observed between the curcumin-treated and control groups in cleavage rate, the proportion of oocytes reaching more than two cells, or blastocyst formation on day 8. These findings indicate that, under the experimental conditions of this study, curcumin does not impair the fertilization capacity of bull sperm. CI - (c) 2026. The Author(s). FAU - Cojkic, Aleksandar AU - Cojkic A AD - Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, 75007, Sweden. aleksandar.cojkic@slu.se. FAU - Sajecka, Olga AU - Sajecka O AD - Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, 75007, Sweden. FAU - Sjunnesson, Ylva AU - Sjunnesson Y AD - Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, 75007, Sweden. FAU - Morrell, Jane M AU - Morrell JM AD - Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Uppsala, 75007, Sweden. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Acta Vet Scand JT - Acta veterinaria Scandinavica JID - 0370400 RN - IT942ZTH98 (Curcumin) SB - IM MH - Animals MH - *Curcumin/pharmacology MH - *Fertilization in Vitro/veterinary/drug effects MH - Male MH - Cattle MH - *Semen/drug effects MH - Spermatozoa/drug effects MH - Female MH - Sperm Motility/drug effects MH - Semen Analysis/veterinary OTO - NOTNLM OT - Bull semen OT - Curcumin OT - IVF COIS- Declarations. Ethical approval and consent to participate: Ethical approval was not required for the study involving animals in accordance with the local legislation and institutional requirements because bull semen collection is a recognized husbandry procedure and does not require ethical approval in Sweden. No experimental or invasive procedures were performed in vivo, and animals were not slaughtered for the purpose of this study. The study was conducted in accordance with European Union legislation on the protection of animals during transport (Council Regulation (EC) No. 1/2005) and at the time of slaughter (Council Regulation (EC) No. 1099/2009). Consequently, the study did not fall within the scope of Directive 2010/63/EU on the protection of animals used for scientific purposes and this practice aligns with local regulations, which consider such materials as non-animal subjects for ethical review. The materials used in this study were not obtained from client-owned animals; therefore no consent was needed. Semen sample used in this study was commercially available semen from an Artificial Insemination centre. Bovine ovaries were obtained from cattle slaughtered for human consumption at an accredited commercial abattoir. Animals originated from standard commercial production systems and were handled and slaughtered following routine industry practices. All sample collection was carried out under standard commercial conditions. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:16 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/15 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/30 01:16 [entrez] AID - 10.1186/s13028-026-00890-5 [pii] AID - 10.1186/s13028-026-00890-5 [doi] PST - epublish SO - Acta Vet Scand. 2026 Oct 29;68(1):47. doi: 10.1186/s13028-026-00890-5. PMID- 42958593 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 2524-4655 (Electronic) IS - 2524-4655 (Linking) VI - 8 IP - 1 DP - 2026 Jul 30 TI - One Health investigation of anthrax outbreaks in Uganda, 2022-2024: real-time PCR detection of Bacillus anthracis virulence-associated targets. LID - 53 [pii] LID - 10.1186/s42522-026-00231-6 [doi] AB - BACKGROUND: Anthrax remains an important zoonotic disease in Uganda, where recurrent outbreaks affect livestock-dependent communities and require coordinated human, animal, environmental, and laboratory response. Although real-time polymerase chain reaction (PCR) is increasingly used for outbreak confirmation, multi-year evidence on the distribution and concordance of virulence-associated molecular targets among outbreak-investigation specimens remains limited. This study characterised detection of the pagA and capC targets among specimens generated during One Health anthrax outbreak investigations in Uganda between 2022 and 2024. METHODS: A retrospective descriptive analysis of secondary epidemiological and laboratory data from 194 specimens collected opportunistically during anthrax outbreak investigations was conducted. Real-time PCR assays targeted pagA, associated with plasmid pXO1, and capC, associated with plasmid pXO2. The primary outcome was dual-target positivity, defined as detection of both targets in the same specimen. Descriptive analyses characterised target detection and positivity patterns. Exploratory univariable modified Poisson regression with robust standard errors estimated crude prevalence ratios (CPRs) and 95% confidence intervals. RESULTS: Of the 194 specimens tested, 31 (16.0%) were positive for pagA and 29 (14.9%) for capC. Twenty-nine specimens (14.9%) were positive for both targets and met the dual-target positivity criterion. Two specimens were pagA-positive/capC-negative, while none were pagA-negative/capC-positive. Annual dual-target positivity was 33.3% in 2022, 11.8% in 2023, and 13.4% in 2024. No statistically significant differences were observed by species, specimen type, region, or selected district. Compared with 2022, dual-target positivity was lower in 2024 (CPR = 0.40; p = 0.021), whereas the difference observed in 2023 was not statistically significant (CPR = 0.35; p = 0.071). CONCLUSION AND RECOMMENDATIONS: Dual-target real-time PCR identified specimens containing both pagA and capC during anthrax outbreak investigations in Uganda, with limited single-target discordance. Findings reflect patterns among opportunistically collected outbreak-investigation specimens and should not be interpreted as population-level prevalence or evidence of geographic hotspots. Strengthening quality-assured molecular confirmation, specimen referral, standardised outbreak documentation, and cross-sector One Health coordination remains important for anthrax surveillance and response. CI - (c) 2026. The Author(s). FAU - Omodo, Michael AU - Omodo M AUID- ORCID: 0000-0003-4961-1980 AD - Department of Disease Control and Environmental Health, School of Public Health, Makerere University, Kampala, P. O. Box 7072, Uganda. FAU - Lemba, Heri AU - Lemba H AUID- ORCID: 0009-0003-6288-0808 AD - Department of Disease Control and Environmental Health, School of Public Health, Makerere University, Kampala, P. O. Box 7072, Uganda. lembaheri387@gmail.com. FAU - Buregyeya, Esther AU - Buregyeya E AUID- ORCID: 0000-0002-4410-8027 AD - Department of Disease Control and Environmental Health, School of Public Health, Makerere University, Kampala, P. O. Box 7072, Uganda. FAU - Isingoma, Emmanuel AU - Isingoma E AD - National Animal Disease Diagnostics and Epidemiology Centre (NADDEC), Ministry of Agriculture, Animal Industry and Fisheries, Kampala, Uganda. FAU - Kayondo, Andrew AU - Kayondo A AD - Department of Disease Control and Environmental Health, School of Public Health, Makerere University, Kampala, P. O. Box 7072, Uganda. FAU - Muleme, James AU - Muleme J AUID- ORCID: 0000-0001-8967-7031 AD - Department of Disease Control and Environmental Health, School of Public Health, Makerere University, Kampala, P. O. Box 7072, Uganda. FAU - Musoke, David AU - Musoke D AUID- ORCID: 0000-0003-3262-3918 AD - Department of Disease Control and Environmental Health, School of Public Health, Makerere University, Kampala, P. O. Box 7072, Uganda. FAU - Kiggundu, Gladys Nakanjako AU - Kiggundu GN AD - National Animal Disease Diagnostics and Epidemiology Centre (NADDEC), Ministry of Agriculture, Animal Industry and Fisheries, Kampala, Uganda. LA - eng PT - Journal Article DEP - 20260730 PL - England TA - One Health Outlook JT - One health outlook JID - 101769253 OTO - NOTNLM OT - Anthrax OT - Bacillus anthracis OT - One Health OT - Outbreak investigation OT - Real-time PCR OT - Uganda OT - capC OT - pagA COIS- Declarations. Ethical approval: This study, titled "One Health investigation of anthrax outbreaks in Uganda, 2022-2024: real-time PCR detection of Bacillus anthracis virulence-associated targets" received ethical approval from the Ethics Committee of the Ministry of Agriculture, Animal Industry and Fisheries (MAAIF), Uganda. Given that anthrax is a notifiable disease and considered a public health priority in Uganda, the research was conducted in accordance with national regulations and guidelines governing zoonotic disease outbreak response and control. All necessary permissions and approvals were obtained before the commencement of sample collection, laboratory investigations and data analysis. Consent for publication: The authors declare consent for publication of this article in the One Health Outlook Journal. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:16 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/12/15 00:00 [received] PHST- 2026/07/13 00:00 [accepted] PHST- 2026/10/30 01:16 [entrez] AID - 10.1186/s42522-026-00231-6 [pii] AID - 10.1186/s42522-026-00231-6 [doi] PST - epublish SO - One Health Outlook. 2026 Jul 30;8(1):53. doi: 10.1186/s42522-026-00231-6. PMID- 42958588 OWN - NLM STAT- In-Process LR - 20261030 IS - 1466-609X (Electronic) IS - 1364-8535 (Linking) VI - 30 IP - 1 DP - 2026 Oct 29 TI - ICU-led remote vital sign trends monitoring of selected non-ICU-patients: a first year experience. LID - 552 [pii] LID - 10.1186/s13054-026-06426-7 [doi] FAU - Koning, Mark V AU - Koning MV AUID- ORCID: 0000-0002-5465-3504 AD - Anaesthesiologist-Intensivist, DESA, EDIC, Rijnstate Hospital, Arnhem, The Netherlands. Markkoning66@hotmail.com. FAU - Peelen, Roel V AU - Peelen RV AUID- ORCID: 0000-0002-6073-540X AD - Military Physician, Ministery of Defence, The Hague, The Netherlands. LA - eng PT - Letter DEP - 20261029 PL - England TA - Crit Care JT - Critical care (London, England) JID - 9801902 SB - IM COIS- Declarations. Ethics approval and consent to participate: Not applicable, because this paper involves an evaluation and not a study, it does not fall under the merit of the Dutch Medical Research Involving Human Subjects Act (WMO). Consent for the use of patient data and publication was part of the informed consent for clinical application of remote monitoring. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 01:16 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/09 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/30 01:16 [entrez] AID - 10.1186/s13054-026-06426-7 [pii] AID - 10.1186/s13054-026-06426-7 [doi] PST - epublish SO - Crit Care. 2026 Oct 29;30(1):552. doi: 10.1186/s13054-026-06426-7. PMID- 42958585 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2045-7634 (Electronic) IS - 2045-7634 (Linking) VI - 15 IP - 11 DP - 2026 Nov TI - Surgical Management and Outcomes of Relapsed and Refractory Hepatoblastoma: An Institutional Case Series. LID - e72453 [pii] LID - 10.1002/cam4.72453 [doi] AB - Relapse occurs in 10%-20% of children with hepatoblastoma and is associated with inferior outcomes, yet standardized management remains limited. Defining risk factors and relapse patterns, as well as the role of surgery, particularly transplantation, is critical to optimizing survival. A retrospective review was performed of patients aged 0-18 years diagnosed with hepatoblastoma from 2007 to 2024. Demographics, tumor characteristics, treatment course, relapse patterns, and outcomes were collected. Analyses included univariable and multivariable logistic regression, Kaplan-Meier survival estimation, and log-rank testing. Among 135 patients, 98 (72.6%) achieved remission without relapse, 32 (23.7%) relapsed, and 5 (3.7%) experienced primary progression without remission. Older age, PRETEXT (pretreatment extent of disease) IV, metastatic disease at diagnosis, and macrovascular invasion were associated with relapse on univariable analysis, but only older age remained independently associated with relapse (adjusted odds ratio 1.27 per year, 95% confidence interval 1.05-1.53); PRETEXT IV and metastatic disease were not significantly associated with overall survival. Lung-only relapse demonstrated favorable survival (100% alive), whereas combined liver and lung or extrahepatic relapse had poorer outcomes (40%-44% alive), although survival did not differ significantly across relapse sites (p = 0.15). Of 54 liver transplant recipients, 44 underwent upfront transplantation, and 10 underwent salvage transplantation, with no significant difference in overall survival (p = 0.90), although the salvage group was small. Relapse or progression after upfront transplantation occurred in 27% of recipients; lung-only relapse again conferred favorable survival. Long-term survival is achievable in relapsed hepatoblastoma, particularly for isolated pulmonary relapse. Older age, likely a surrogate for adverse tumor biology, was associated with relapse but did not preclude survival when aggressive multimodal therapy was used. Salvage transplantation achieved durable survival in selected patients and should be considered for unresectable intrahepatic relapse when extrahepatic disease is absent or controlled. CI - (c) 2026 The Author(s). Cancer Medicine published by John Wiley & Sons Ltd. FAU - Ray, Marissa AU - Ray M AUID- ORCID: 0009-0007-4345-9531 AD - Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA. AD - Department of Surgery, University of Cincinnati Medical Center, Cincinnati, Ohio, USA. FAU - Stetson, Alyssa AU - Stetson A AD - Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA. AD - Department of Surgery, Brigham and Women's Hospital, Boston, Massachusetts, USA. FAU - Noriega, Nicolas AU - Noriega N AUID- ORCID: 0000-0001-6573-1611 AD - Department of Surgery, University of Cincinnati Medical Center, Cincinnati, Ohio, USA. FAU - Hartman, Stephen AU - Hartman S AUID- ORCID: 0000-0001-5732-5832 AD - Department of Surgery, University of Cincinnati Medical Center, Cincinnati, Ohio, USA. FAU - Brown, Colton AU - Brown C AUID- ORCID: 0009-0004-4331-8825 AD - Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA. AD - Department of Surgery, University of Cincinnati Medical Center, Cincinnati, Ohio, USA. FAU - Schepers, Emily AU - Schepers E AUID- ORCID: 0000-0001-5567-262X AD - Department of Surgery, University of Cincinnati Medical Center, Cincinnati, Ohio, USA. FAU - Farooqui, Zishaan AU - Farooqui Z AUID- ORCID: 0000-0001-7914-0425 AD - Department of Surgery, University of Cincinnati Medical Center, Cincinnati, Ohio, USA. FAU - Towbin, Alexander J AU - Towbin AJ AUID- ORCID: 0000-0003-1729-5071 AD - Department of Radiology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA. AD - Department of Radiology, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA. FAU - Somers, Katherine M AU - Somers KM AUID- ORCID: 0000-0001-8683-9488 AD - Cancer and Blood Diseases Institute, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA. FAU - Ranganathan, Sarangarajan AU - Ranganathan S AUID- ORCID: 0000-0001-6214-9167 AD - Department of Pathology and Laboratory Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA. FAU - Geller, James AU - Geller J AUID- ORCID: 0000-0001-5181-116X AD - Peckham Center for Cancer and Blood Disorders, Rady Children's Hospital, San Diego, California, USA. FAU - Evans, Sue AU - Evans S AUID- ORCID: 0000-0001-8342-8472 AD - Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA. FAU - Merola, Jonathan AU - Merola J AD - Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA. FAU - Bondoc, Alexander J AU - Bondoc AJ AUID- ORCID: 0000-0002-9802-5948 AD - Department of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA. FAU - Tiao, Greg AU - Tiao G AUID- ORCID: 0000-0001-8099-2596 AD - Division of Abdominal Transplantation, Stanford Medicine Children's Health, Palo Alto, California, USA. LA - eng PT - Journal Article PL - United States TA - Cancer Med JT - Cancer medicine JID - 101595310 SB - IM MH - Humans MH - *Hepatoblastoma/surgery/mortality/pathology MH - Female MH - Child, Preschool MH - Male MH - *Liver Neoplasms/surgery/pathology/mortality MH - Infant MH - *Neoplasm Recurrence, Local/surgery/pathology/mortality MH - Child MH - Retrospective Studies MH - Adolescent MH - Infant, Newborn MH - Treatment Outcome MH - Liver Transplantation MH - Salvage Therapy MH - Risk Factors OTO - NOTNLM OT - hepatoblastoma OT - metastasectomy OT - relapse OT - salvage transplantation OT - surgical outcomes OT - survival analysis EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:16 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/19 00:00 [revised] PHST- 2026/06/14 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/30 01:16 [entrez] AID - e72453 [pii] AID - 10.1002/cam4.72453 [doi] PST - ppublish SO - Cancer Med. 2026 Nov;15(11):e72453. doi: 10.1002/cam4.72453. PMID- 42958581 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1756-0500 (Electronic) IS - 1756-0500 (Linking) VI - 19 IP - 1 DP - 2026 Sep 9 TI - Optimizing a human forensic STR panel for non-invasive genetic monitoring of chimpanzees (Pan troglodytes). LID - 388 [pii] LID - 10.1186/s13104-026-07997-y [doi] AB - OBJECTIVE: Accurate molecular genotyping is essential for conservation genetics, population monitoring, pedigree reconstruction, and wildlife forensic investigations in endangered primates. Although SNP-based approaches are increasingly available, microsatellites remain among the most practical tools for non-invasive individual identification because of their high allelic diversity and strong discriminatory power from relatively few loci. Here, we present a standardized optimization framework for adapting the human forensic GlobalFiler PCR Amplification Kit to non-invasive chimpanzee genotyping. RESULTS: Using blood and fecal DNA from captive and wild chimpanzees in Cameroon, we integrated DNA quality screening, chimpanzee-specific allele binning, replicate consensus genotyping, statistical evaluation of locus performance, and composite marker ranking to develop a robust 16-locus panel for individual identification. Estimated marginal means were calculated from the fitted beta regression models to assess amplification success, allelic dropout, and false allele rates across sample types and loci. These metrics, combined with measures of discriminatory power, identified D10S1248, FGA, D2S441, and D3S1358 as the highest-performing loci. The optimized panel achieved 95.4% amplification success with low allelic dropout (0.08) and false allele (0.02) rates. It identified 64 unique individuals (39 males and 25 females) and showed high discriminatory power (P(ID(sibs)) = 1.2 x 10(-)(6)). This workflow is easily transferable and can complement SNP genotyping, metabarcoding, microbiome analyses, and other molecular approaches in a wide range of non-invasive genetic studies. CI - (c) 2026. The Author(s). FAU - Paul, Shrutarshi AU - Paul S AUID- ORCID: 0000-0002-3950-7525 AD - Department of Ecology and Conservation Biology, Texas A&M University, College Station, TX, USA. shrutarshi@tamu.edu. FAU - Kentatchime, Fabrice AU - Kentatchime F AD - Department of Ecology and Conservation Biology, Texas A&M University, College Station, TX, USA. FAU - Andres-Bray, Tyler AU - Andres-Bray T AD - Department of Ecology and Evolutionary Biology, University of California, Los Angeles, CA, USA. FAU - Ghosh, Tista AU - Ghosh T AD - Department of Ecosystem Science and Management, University of Northern British Columbia, Prince George, British Columbia, Canada. FAU - Couto, Giovana Naira Pereira AU - Couto GNP AD - Department of Ecology and Conservation Biology, Texas A&M University, College Station, TX, USA. FAU - Njabo, Kevin AU - Njabo K AD - Department of Ecology and Conservation Biology, Texas A&M University, College Station, TX, USA. FAU - Gonder, Mary Katherine AU - Gonder MK AUID- ORCID: 0000-0003-4190-4682 AD - Department of Ecology and Conservation Biology, Texas A&M University, College Station, TX, USA. katy.gonder@ag.tamu.edu. LA - eng PT - Journal Article DEP - 20260909 PL - England TA - BMC Res Notes JT - BMC research notes JID - 101462768 RN - 9007-49-2 (DNA) SB - IM MH - Animals MH - *Pan troglodytes/genetics MH - *Microsatellite Repeats/genetics MH - Female MH - Humans MH - Male MH - *Forensic Genetics/methods MH - *Genotyping Techniques/methods MH - Genotype MH - Cameroon MH - DNA/genetics MH - Polymerase Chain Reaction/methods MH - Alleles MH - Feces/chemistry OTO - NOTNLM OT - Cameroon OT - Chimpanzee genetics OT - Conservation genetics OT - Fecal DNA OT - Genotyping error OT - GlobalFiler OT - Microsatellites OT - Non-invasive sampling OT - STRs OT - wildlife forensics COIS- Declarations. Ethics approval and consent to participate: All animal procedures were conducted in accordance with applicable national regulations and institutional guidelines for the ethical use of animals in research. Blood and fecal sample collection was carried out under permits issued by the Ministry of Forestry and Wildlife (MINFOF) (Permit No: 2306) and the Ministry of Scientific Research and Innovation (MINRESI) (Permint No: 000096), Cameroon. Sample importation into the United States was authorized by the U.S. Centers for Disease Control and Prevention (CDC). Consent to participate was not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. Artificial intelligence statement: During the preparation of this manuscript, the authors used OpenAI ChatGPT to assist with language editing, manuscript organization, and development of a preliminary graphical abstract concept. All data collection, statistical analyses, interpretation, and final figure preparation were conducted by the authors without the use of AI-generated analyses. The authors reviewed, revised, and approved all content and take full responsibility for the published work. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:15 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/07/10 00:00 [received] PHST- 2026/08/25 00:00 [accepted] PHST- 2026/10/30 01:15 [entrez] AID - 10.1186/s13104-026-07997-y [pii] AID - 10.1186/s13104-026-07997-y [doi] PST - epublish SO - BMC Res Notes. 2026 Sep 9;19(1):388. doi: 10.1186/s13104-026-07997-y. PMID- 42958575 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1749-8090 (Electronic) IS - 1749-8090 (Linking) VI - 21 IP - 1 DP - 2026 Aug 3 TI - PSMD12 is a novel potential biomarker for lung adenocarcinoma and associated with prognosis in lung adenocarcinoma patients. LID - 864 [pii] LID - 10.1186/s13019-026-04601-z [doi] AB - BACKGROUND: This study aims to comprehensively evaluate the expression pattern of PSMD12 in lung adenocarcinoma (LUAD) and its impact on prognosis, and to explore its potential application value in the diagnosis and treatment of LUAD. METHODS: This study employed bioinformatics analysis and molecular biology experiments to comprehensively investigate the expression pattern of the PSMD12 gene in LUAD and its association with LUAD patients' clinical features. First, we evaluated the differential expression of PSMD12 in 44 different tumor types and its relationship with the prognosis of LUAD patients. GO, KEGG, and GSEA were used to analyze the function of PSMD12. Secondly, TIMER was applied to evaluated the relationship between PSMD12 gene expression and immune infiltration characteristics in LUAD patients. Finally, RT-qPCR, CCK-8, clone formation assay, wound healing assay, and Transwell assay were used to verify the effects of knockdown PSMD12 expression on the proliferation, migration, and invasion abilities of LUAD cells. RESULTS: Survival analysis results showed that high PSMD12 gene expression levels were significantly correlated with the poor prognosis of LUAD patients. The importance of PSMD12 in key metabolic pathways and disease processes such as neuroactive ligand-receptor interaction, taste and olfactory transduction, and steroid hormone biosynthesis was revealed. Immune infiltration analysis results showed that PSMD12 expression was negatively correlated with M2 macrophages, revealing the potential role of PSMD12 in regulating the immune microenvironment of LUAD. In vitro experiments verified the functional role of the PSMD12 in LUAD cells, indicating that knockdown of PSMD12 weakened the proliferation, migration, and invasion abilities of LUAD A549 and H1299 cells. CONCLUSIONS: High expression of PSMD12 may be closely related to poor prognosis in LUAD patients and promotes the malignant progression of LUAD by enhancing the proliferation, migration, and invasion of LUAD cells. CI - (c) 2026. The Author(s). FAU - Lu, Shuyu AU - Lu S AD - Department of Cardiothoracic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China. FAU - Li, Yue AU - Li Y AUID- ORCID: 0009-0007-6994-5693 AD - Department of Cardiothoracic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China. FAU - Peng, Huajian AU - Peng H AD - Department of Cardiothoracic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China. FAU - Gao, Xiang AU - Gao X AD - Department of Cardiothoracic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China. FAU - Xu, Zhanyu AU - Xu Z AD - Department of Cardiothoracic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China. FAU - Zhou, Huafu AU - Zhou H AUID- ORCID: 0000-0002-2267-3164 AD - Department of Cardiothoracic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China. FAU - Guo, Jianji AU - Guo J AD - Department of Cardiothoracic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China. guojianji@163.com. FAU - Yang, Nuo AU - Yang N AUID- ORCID: 0009-0006-4623-8913 AD - Department of Cardiothoracic Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, Guangxi, China. yangnuo@gxmu.edu.cn. LA - eng GR - Z20211032/Self-financed scientific research of Guangxi Zhuang Autonomous Region Health Commission/ GR - 2024GXNSFAA010101/Joint Project on Regional High Incidence Diseases Research of Guangxi Natural Science Foundation/ PT - Journal Article DEP - 20260803 PL - England TA - J Cardiothorac Surg JT - Journal of cardiothoracic surgery JID - 101265113 RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - Prognosis MH - *Biomarkers, Tumor/genetics/biosynthesis MH - *Lung Neoplasms/genetics/metabolism/diagnosis/mortality MH - *Adenocarcinoma of Lung/genetics/metabolism/diagnosis/mortality MH - Female MH - Male MH - Gene Expression Regulation, Neoplastic MH - Cell Proliferation MH - Cell Movement OTO - NOTNLM OT - Cell migration and invasion OT - Immune infiltration OT - Lung adenocarcinoma OT - PSMD12 OT - Prognosis COIS- Declarations. Ethics approval and consent to participate: This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of First Affiliated Hospital of Guangxi Medical University [No.2024-E495-01]. Informed consent was obtained from all individual participants included in the study. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:15 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/11/15 00:00 [received] PHST- 2026/07/11 00:00 [accepted] PHST- 2026/10/30 01:15 [entrez] AID - 10.1186/s13019-026-04601-z [pii] AID - 10.1186/s13019-026-04601-z [doi] PST - epublish SO - J Cardiothorac Surg. 2026 Aug 3;21(1):864. doi: 10.1186/s13019-026-04601-z. PMID- 42958566 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 2052-1847 (Print) IS - 2052-1847 (Linking) VI - 18 IP - 1 DP - 2026 Aug 3 TI - Trends and fluctuations in technical performance in the 2010-2022 FIBA women's basketball world cup. LID - 537 [pii] LID - 10.1186/s13102-026-01939-8 [doi] AB - This study selected the quarterfinals, semifinals, and finals of the FIBA Women's Basketball World Cup in 2010, 2014, 2018, and 2022, totaling 32 games, and aimed to examine the trends, fluctuations, and differences in technical performances of the Women's Basketball World Cup from 2010 to 2022 using the Grey Model (GM), Kruskal-Wallis test, and coefficient of variation (CV). The results showed that two-point field goal attempts, two-point field goals made, and offensive rebounds exhibited a declining trend with low fluctuation. Assists and blocks both showed an increasing trend and high fluctuation. Three-point field goal attempts and three-point field goals both showed an increasing trend, although the former had moderate fluctuation, while the latter had high fluctuation. Steals showed an increasing trend with low fluctuation, while turnovers showed a declining trend with high fluctuation, while turnovers showed a declining trend with high fluctuation. However, the GM (1,1) models for steals and turnovers exhibited suboptimal fitting performance; consequently, their trend directions are presented as provisional reference estimates rather than definitive conclusions. Furthermore, free throw attempts, free throws made, and defensive rebounds showed an increasing trend with moderate fluctuation. Finally, significant differences were found between game editions in terms of assists (p < 0.001, large effect) and blocks (p < 0.05, medium effect). Overall, the study results indicate that technical performance in the Women's Basketball World Cup exhibit both upward and downward trends. The significant changes occurred between different years of games in terms of team coordination and shot-blocking ability. Additionally, techniques showed three different levels of fluctuation: low, moderate, and high. This study provided a theoretical support for the related longitudinal analysis of basketball techniques. In addition, it can also serve as a potential reference for coaches to optimize training programs and game strategies. CI - (c) 2026. The Author(s). FAU - Zhang, Yihong AU - Zhang Y AD - School of Athletic Performance, Shanghai University of Sport, Shanghai, China. FAU - Wang, Jie AU - Wang J AD - School of Athletic Performance, Shanghai University of Sport, Shanghai, China. 153627313@qq.com. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - BMC Sports Sci Med Rehabil JT - BMC sports science, medicine & rehabilitation JID - 101605016 OTO - NOTNLM OT - Basketball OT - Grey Model OT - Longitudinal analysis OT - Technical Performance OT - Trends COIS- Declarations. Ethics approval to consent to participate: Not applicable. Consent for publication: Not applicable. Competing interest: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:15 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/06/08 00:00 [received] PHST- 2026/07/29 00:00 [accepted] PHST- 2026/10/30 01:15 [entrez] AID - 10.1186/s13102-026-01939-8 [pii] AID - 10.1186/s13102-026-01939-8 [doi] PST - epublish SO - BMC Sports Sci Med Rehabil. 2026 Aug 3;18(1):537. doi: 10.1186/s13102-026-01939-8. PMID- 42958562 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1752-1947 (Electronic) IS - 1752-1947 (Linking) VI - 20 IP - 1 DP - 2026 Aug 2 TI - Bone-preserving ankle arthrodesis after infected pilon fracture nonunion: lessons from tapered distal docking and fibular-supported reconstruction. LID - 664 [pii] LID - 10.1186/s13256-026-06436-7 [doi] AB - BACKGROUND: Infected nonunion after severe pilon fracture remains a major challenge in limb reconstruction, particularly when chronic infection and repeated debridement result in severe metaphyseal bone loss and compromised distal tibial morphology. In such cases, conventional resection to create a flat arthrodesis surface may enlarge the defect and substantially prolong bone transport duration. We report a bone-preserving reconstructive strategy using tapered distal docking and fibular-assisted ankle arthrodesis during bone transport for infected pilon fracture nonunion. CASE PRESENTATION: A 25-year-old man developed chronic infected nonunion after a severe AO/OTA type 43-C3 pilon fracture treated with multiple operations at another institution. Methicillin-sensitive Staphylococcus aureus infection, repeated debridement procedures, and implant removal resulted in persistent nonunion with progressive tapering of the distal tibia. Computed tomography demonstrated a markedly cone-shaped distal tibial morphology with an extremely limited anticipated tibiotalar contact area, while the residual cortex retained structural continuity. Conventional resection to obtain a flat docking surface would have required approximately 40 mm of additional tibial shortening, substantially prolonging the planned bone transport phase. Because the residual tapered cortex appeared biologically viable and mechanically reconstructable with planned augmentation, we intentionally preserved the native distal tibial morphology rather than sacrificing additional bone for geometric simplification. Limb salvage using bone transport and ankle arthrodesis with circular external fixation was performed. To compensate for the limited docking area, fibular osteotomy and fibular-assisted ankle arthrodesis were incorporated as a lateral load-sharing support. After staged debridement, antibiotic spacer placement, proximal tibial corticotomy, gradual bone transport, and fibular-supported docking, successful union and ankle arthrodesis were achieved without recurrent infection. At final follow-up 6 years postoperatively, durable union and maintained alignment were confirmed radiographically without adjacent joint degeneration or recurrent infection. The patient reported no pain (VAS 0), achieved an AOFAS ankle-hindfoot score of 92, and returned to physically demanding construction work without orthotic support. CONCLUSIONS: This report highlights an important educational principle in limb-salvage reconstruction after infected pilon fracture nonunion. In highly selected patients with controlled infection, viable residual cortex, favorable computed tomography morphology, and feasible mechanical augmentation, severely tapered distal tibial cortex may be preserved and incorporated into ankle arthrodesis rather than routinely resected for geometric simplification. This strategy should not be regarded as broadly applicable, but as a selected bone-preserving option within individualized limb-salvage reconstruction. CI - (c) 2026. The Author(s). FAU - Nozaka, Koji AU - Nozaka K AUID- ORCID: 0000-0003-0238-8929 AD - Department of Orthopaedic Surgery, Akita University Graduate School of Medicine, Akita, Japan. kk-nozaka@mue.biglobe.ne.jp. FAU - Shirahata, Tsuyoshi AU - Shirahata T AD - Department of Orthopaedic Surgery, Akita University Graduate School of Medicine, Akita, Japan. FAU - Yuasa, Yusuke AU - Yuasa Y AD - Department of Orthopaedic Surgery, Akita University Graduate School of Medicine, Akita, Japan. FAU - Harata, Shuntaro AU - Harata S AD - Department of Orthopaedic Surgery, Akita University Graduate School of Medicine, Akita, Japan. FAU - Morishita, Y O AU - Morishita YO AD - Department of Orthopaedic Surgery, Akita University Graduate School of Medicine, Akita, Japan. FAU - Miyakoshi, Naohisa AU - Miyakoshi N AD - Department of Orthopaedic Surgery, Akita University Graduate School of Medicine, Akita, Japan. LA - eng PT - Case Reports PT - Journal Article DEP - 20260802 PL - England TA - J Med Case Rep JT - Journal of medical case reports JID - 101293382 SB - IM MH - Humans MH - Male MH - *Arthrodesis/methods MH - Adult MH - *Tibial Fractures/surgery/diagnostic imaging/complications MH - Fibula/surgery MH - *Fractures, Ununited/surgery/diagnostic imaging MH - *Ankle Joint/surgery/diagnostic imaging MH - Hardware Removal MH - Debridement MH - *Staphylococcal Infections/surgery MH - Treatment Outcome MH - Tomography, X-Ray Computed MH - *Plastic Surgery Procedures/methods OTO - NOTNLM OT - Ankle arthrodesis OT - Bone transport OT - Circular external fixation OT - Docking site OT - Fibular support OT - Ilizarov technique OT - Infected nonunion OT - Limb salvage OT - Pilon fracture OT - Tapered distal tibia COIS- Declarations. Ethics approval and consent to participate: Ethical approval for this study was obtained from the Ethics Committee of Akita University Graduate School of Medicine (Approval No. 3403). The study was conducted in accordance with the Declaration of Helsinki. Consent for publication: Written informed consent was obtained from the patient for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal. Competing interest: The authors declare that they have no competing interest. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:14 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/05/19 00:00 [received] PHST- 2026/07/23 00:00 [accepted] PHST- 2026/10/30 01:14 [entrez] AID - 10.1186/s13256-026-06436-7 [pii] AID - 10.1186/s13256-026-06436-7 [doi] PST - epublish SO - J Med Case Rep. 2026 Aug 2;20(1):664. doi: 10.1186/s13256-026-06436-7. PMID- 42958536 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1479-5876 (Electronic) IS - 1479-5876 (Linking) VI - 24 IP - 1 DP - 2026 Oct 21 TI - Angiotensinogen as a molecular hub in interorgan communication. LID - 1369 [pii] LID - 10.1186/s12967-026-09074-1 [doi] AB - Angiotensinogen (AGT) is the sole precursor of angiotensin peptides, its physiological relevance is not determined solely by precursor abundance. Although the liver supplies the bulk of circulating AGT, the protein detected in peripheral tissues may arise from local synthesis, vascular retention, glomerular filtration, barrier crossing, or receptor-mediated uptake. Moreover, the molecular form in which AGT exists carries functional implications: intact AGT and des(Ang I)AGT produce effects that are distinct from-and under certain conditions, opposing to-those of angiotensin II (Ang II). This review repositions AGT as a context-sensitive mediator of interorgan crosstalk, with a clear demarcation between preclinical models and human data. Studies using source-specific knockout and lineage tracing in mice offer the strongest causal evidence for liver-to-kidney AGT trafficking; most other proposed axes lack such granular resolution. Human genetic loss-of-function studies have definitively established AGT's indispensability for renal morphogenesis and blood-pressure regulation, while observational cohorts and biomarker studies rarely determine its tissue origin or delivery route. Ultimately, the functional output of AGT across organ systems depends on its source, molecular state, local accessibility, enzymatic processing, and the receptor environment. Therapeutic agents that target hepatocytes, such as zilebesiran and tonlamarsen, produce durable AGT suppression and could potentially ease treatment burden relative to conventional daily angiotensin-converting enzyme (ACE) inhibitors or angiotensin receptor blockers (ARBs). Their slow offset of action, combined with a current lack of solid cardiovascular and renal outcome data, raises an unresolved but critical question: what is the safety margin for AGT reduction before renal self-regulation, fluid-electrolyte balance, or pressor responses to physiological stress become compromised? Overall, AGT does not function as a ubiquitous interorgan signaling molecule; instead, it functions as an upstream determinant whose impact is highly context-dependent, shaped by its site of production, route of dissemination, and mode of processing. This review systematically differentiates experimentally established source-to-target circuits from plausible but as-yet-unconfirmed pathways and outlines the mechanistic and clinical benchmarks that must be met for the safe, tissue-selective application of AGT-targeted therapy. CI - (c) 2026. The Author(s). FAU - Zhou, Jianan AU - Zhou J AD - Department of Cardiology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China. AD - State Key Laboratory of Transvascular Implantation Devices, Hangzhou, China. AD - Heart Regeneration and Repair Key Laboratory of Zhejiang Province, Hangzhou, China. AD - Transvascular Implantation Devices Research Institute, Hangzhou, China. AD - Binjiang Institute of Zhejiang University, Hangzhou, China. FAU - Ye, Ximing AU - Ye X AD - Department of Cardiology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China. AD - State Key Laboratory of Transvascular Implantation Devices, Hangzhou, China. AD - Heart Regeneration and Repair Key Laboratory of Zhejiang Province, Hangzhou, China. AD - Transvascular Implantation Devices Research Institute, Hangzhou, China. AD - Binjiang Institute of Zhejiang University, Hangzhou, China. FAU - Wang, Yuying AU - Wang Y AD - Department of Cardiology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China. AD - State Key Laboratory of Transvascular Implantation Devices, Hangzhou, China. AD - Heart Regeneration and Repair Key Laboratory of Zhejiang Province, Hangzhou, China. AD - Transvascular Implantation Devices Research Institute, Hangzhou, China. AD - Binjiang Institute of Zhejiang University, Hangzhou, China. FAU - Hong, Min AU - Hong M AD - Department of Cardiology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China. AD - State Key Laboratory of Transvascular Implantation Devices, Hangzhou, China. AD - Heart Regeneration and Repair Key Laboratory of Zhejiang Province, Hangzhou, China. AD - Transvascular Implantation Devices Research Institute, Hangzhou, China. AD - Binjiang Institute of Zhejiang University, Hangzhou, China. FAU - Rong, Jiabing AU - Rong J AUID- ORCID: 0000-0002-6804-3408 AD - Department of Cardiology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China. rjb@zju.edu.cn. AD - State Key Laboratory of Transvascular Implantation Devices, Hangzhou, China. rjb@zju.edu.cn. AD - Heart Regeneration and Repair Key Laboratory of Zhejiang Province, Hangzhou, China. rjb@zju.edu.cn. AD - Transvascular Implantation Devices Research Institute, Hangzhou, China. rjb@zju.edu.cn. AD - Binjiang Institute of Zhejiang University, Hangzhou, China. rjb@zju.edu.cn. FAU - Wang, Jianan AU - Wang J AUID- ORCID: 0000-0002-4583-3204 AD - Department of Cardiology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China. wangjianan111@zju.edu.cn. AD - State Key Laboratory of Transvascular Implantation Devices, Hangzhou, China. wangjianan111@zju.edu.cn. AD - Heart Regeneration and Repair Key Laboratory of Zhejiang Province, Hangzhou, China. wangjianan111@zju.edu.cn. AD - Transvascular Implantation Devices Research Institute, Hangzhou, China. wangjianan111@zju.edu.cn. AD - Binjiang Institute of Zhejiang University, Hangzhou, China. wangjianan111@zju.edu.cn. FAU - Xu, Yinchuan AU - Xu Y AUID- ORCID: 0000-0001-9472-7507 AD - Department of Cardiology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310009, China. 2314029@zju.edu.cn. AD - State Key Laboratory of Transvascular Implantation Devices, Hangzhou, China. 2314029@zju.edu.cn. AD - Heart Regeneration and Repair Key Laboratory of Zhejiang Province, Hangzhou, China. 2314029@zju.edu.cn. AD - Transvascular Implantation Devices Research Institute, Hangzhou, China. 2314029@zju.edu.cn. AD - Binjiang Institute of Zhejiang University, Hangzhou, China. 2314029@zju.edu.cn. LA - eng GR - 82270385/National Natural Science Foundation of China/ GR - 81971860/National Natural Science Foundation of China/ GR - 82202358/National Natural Science Foundation of China/ GR - 226-2024-998 00026/Fundamental Research Funds for the Central Universities/ GR - XY2025080/Scientific Research Foundation of Zhejiang A and F University/ PT - Journal Article PT - Review DEP - 20261021 PL - England TA - J Transl Med JT - Journal of translational medicine JID - 101190741 RN - 11002-13-4 (Angiotensinogen) SB - IM MH - Humans MH - Animals MH - *Angiotensinogen/metabolism MH - Liver/metabolism MH - Kidney/metabolism MH - *Organ Specificity OTO - NOTNLM OT - Angiotensinogen OT - Interorgan communication OT - Liver-derived angiotensinogen OT - RNA therapeutics OT - Renin-angiotensin system COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Declaration of generative AI and AI-assisted technologies in the writing process: During the preparation of this manuscript, an AI tool was utilized to assist in the summary of provided reference materials and to refine the manuscript's language, including checking grammar, style, and syntax. The author was responsible for critically evaluating all sources, drafting the entire manuscript, and verifying the final content for accuracy and originality. The author takes full responsibility for the intellectual content of the work presented. Competing interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:13 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/08/04 00:00 [received] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/30 01:13 [entrez] AID - 10.1186/s12967-026-09074-1 [pii] AID - 10.1186/s12967-026-09074-1 [doi] PST - epublish SO - J Transl Med. 2026 Oct 21;24(1):1369. doi: 10.1186/s12967-026-09074-1. PMID- 42958535 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2474 (Electronic) IS - 1471-2474 (Linking) VI - 27 IP - 1 DP - 2026 Aug 4 TI - Biomechanical comparative analysis of minimally invasive decompression and minimally invasive fusion with internal fixation in the treatment of lumbar spinal stenosis with degenerative spondylolisthesis: a finite element study. LID - 938 [pii] LID - 10.1186/s12891-026-10304-2 [doi] AB - BACKGROUND: This study aimed to analyze and compare the biomechanical effects of four different minimally invasive surgical procedures for lumbar spinal stenosis complicated by degenerative spondylolisthesis. METHODS: A static finite element analysis was performed using data from a single healthy volunteer. A validated three-dimensional L1-S1 finite element model was constructed from CT scans of a 26‑year‑old healthy adult male. Based on the health model, the following finite element models were created: lumbar spinal stenosis with degenerative spondylolisthesis (LSS-DS), unilateral biportal endoscopy with ipsilateral decompression only (UBE‑IDO), unilateral biportal endoscopy lumbar interbody fusion (ULIF), minimally invasive transforaminal lumbar interbody fusion (Mis-TLIF), and endoscopic transforaminal lumbar interbody fusion (Endo-TLIF). Using the Hyperwork software, flexion-extension, lateral bending and lateral rotation loading conditions were simulated to compare the biomechanical effects among the models. RESULTS: Compared with the LSS-DS, the UBE‑IDO model showed changes in peak von Mises stress of the L4-L5 intervertebral disc and articular cartilage not exceeding 0.21 MPa. The ULIF model exhibited the highest bone graft stress among the fusion groups. Under the left lateral rotation, the Mis-TLIF demonstrated the highest peak von Mises stress in the internal fixation system(113.62 MPa). Under the right lateral bending, the Mis-TLIF showed the highest cage peak von Mises stress(25.12 MPa). Under the flexion, extension, and rotation, the peak von Mises stress of the L5-S1 articular cartilage in the Endo-TLIF group was the highest. Under the left bending and right rotation conditions, the Mis-TLIF had the highest peak von Mises stress at the L5-S1 articular cartilage. CONCLUSIONS: From a biomechanical perspective, the UBE‑IDO surgery exerts a relatively minor impact on the lumbar spine. ULIF provides a more favorable biomechanical environment for intervertebral fusion. In the fusion model, Endo-TLIF provides a relatively favorable biomechanical environment for the internal fixation. Among the three fusion models, ULIF has the least impact on the biomechanics of adjacent segments. The absolute stress values presented herein are intended only for hypothesis generation, and inferences should be limited to relative trends among surgical models under identical assumptions. CI - (c) 2026. The Author(s). FAU - Liu, Hanqiang AU - Liu H AD - Department of Spinal Surgery, Affiliated Hospital of Shandong Second Medical University, Weifang, Shandong, China. FAU - Xie, Yuliang AU - Xie Y AD - Department of Spinal Surgery, Affiliated Hospital of Shandong Second Medical University, Weifang, Shandong, China. FAU - Hu, Hongtao AU - Hu H AD - Department of Spinal Surgery, Affiliated Hospital of Shandong Second Medical University, Weifang, Shandong, China. FAU - Sun, Jianwei AU - Sun J AD - Department of Spinal Surgery, Affiliated Hospital of Shandong Second Medical University, Weifang, Shandong, China. FAU - Yang, Aijun AU - Yang A AD - Department of Operating Room, Affiliated Hospital of Shandong Second Medical University, Weifang, Shandong, China. FAU - Zhao, Sishun AU - Zhao S AD - Department of Spinal Surgery, Affiliated Hospital of Shandong Second Medical University, Weifang, Shandong, China. FAU - Wang, XiuRong AU - Wang X AD - Department of Operating Room, Affiliated Hospital of Shandong Second Medical University, Weifang, Shandong, China. FAU - Liu, Hongbo AU - Liu H AD - Department of Emergency, Affiliated Hospital of Shandong Second Medical University, Weifang, Shandong, China. FAU - Wu, Changqing AU - Wu C AD - Department of Operating Room, Affiliated Hospital of Shandong Second Medical University, Weifang, Shandong, China. wcq5215@163.com. FAU - Wang, Sheng AU - Wang S AD - Department of Spinal Surgery, Affiliated Hospital of Shandong Second Medical University, Weifang, Shandong, China. fywangsheng@sdsmu.edu.cn. LA - eng GR - 2022YX091/Weifang Science and Technology Development Program Projects/ GR - SDYAL2024122/Shandong Province Education Department Project/ PT - Comparative Study PT - Journal Article DEP - 20260804 PL - England TA - BMC Musculoskelet Disord JT - BMC musculoskeletal disorders JID - 100968565 SB - IM MH - Humans MH - *Finite Element Analysis MH - Male MH - *Spondylolisthesis/surgery/diagnostic imaging/complications/physiopathology MH - *Lumbar Vertebrae/surgery/diagnostic imaging/physiopathology MH - *Spinal Fusion/methods/instrumentation MH - *Decompression, Surgical/methods MH - Biomechanical Phenomena MH - *Spinal Stenosis/surgery/diagnostic imaging/physiopathology/complications MH - *Minimally Invasive Surgical Procedures/methods MH - Adult OTO - NOTNLM OT - Biomechanical effects OT - Degenerative spondylolisthesis OT - Finite element analysis OT - Lumbar spinal stenosis OT - Minimally invasive lumbar fusion surgery COIS- Declarations. Ethics approval and consent to participate: This study has been reviewed by the ethics committee of Affiliated Hospital of Shandong Second Medical University and have been performed in accordance with the ethical standards laid down in an appropriate version of the 1964 Declaration of Helsinki. This study has obtained the informed consent of all participants. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:13 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/03/23 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/30 01:13 [entrez] AID - 10.1186/s12891-026-10304-2 [pii] AID - 10.1186/s12891-026-10304-2 [doi] PST - epublish SO - BMC Musculoskelet Disord. 2026 Aug 4;27(1):938. doi: 10.1186/s12891-026-10304-2. PMID- 42958524 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2458 (Electronic) IS - 1471-2458 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Effectiveness of health mediation to promote organised cancer screening among underserved and under-screened populations in Marseille, France: findings from a repeated cross-sectional survey. LID - 3129 [pii] LID - 10.1186/s12889-026-28710-5 [doi] AB - BACKGROUND: Since 2022, the "13 en Sante" health mediation program has been implemented in Marseille, France, to reduce social health inequalities, in socioeconomically disadvantage neighbourhoods, with a particular emphasis on promoting participation in population-based organised cancer screening programs. Led by multidisciplinary teams including health mediators, nurses, coordinators, and volunteers, the intervention is structured around three core components: (i) territory exploration to develop knowledge of local communities and resources; (ii) culturally appropriate health education delivered through outreach activities to reach underserved populations; and (iii) individualised navigation support tailored to needs and level of autonomy. This study evaluates the effectiveness of this program in promoting breast, colorectal, and cervical cancer screening. METHODS: A controlled before-after design based on two cross-sectional knowledge-attitude-practice surveys was conducted in 2022 and 2024 in intervention or control neighbourhoods. Individuals aged 18-74 were randomly selected and interviewed via door-to-door questionnaires. Weighting was applied to account for stratified sampling and to align age and sex distributions with census data. For each cancer, weighted logistic regression models assessed associations between health mediation exposure and screening uptake and awareness at both individual and population levels. RESULTS: Overall, 4,523 individuals were included across the two cross-sectional surveys. The program successfully reached individuals facing cumulative socioeconomic barriers to healthcare access. No significant population-level association was observed between health mediation and cancer screening uptake or awareness. At the individual level, declared exposure to health mediation was associated with significantly higher uptakes of breast and colorectal cancer screenings (breast: 74% vs. 54%, OR = 2.3 [1.1-4.5]; colorectal: 50% vs. 30%, OR = 2.8 [1.3-5.8]). In addition, colorectal cancer screening awareness was significantly higher among exposed participants (93% vs. 83%, OR = 8.1 [2.1-31]). CONCLUSIONS: This study provides the first evidence that reported individual exposure to a multicomponent health mediation intervention was significantly associated with an important increase in breast and colorectal cancer screening uptake in disadvantaged European neighbourhoods. Although the limitations of this observational study preclude causal conclusions regarding the intervention's effectiveness, these findings suggest that health mediation may be a promising strategy for reducing inequalities in cancer prevention and warrant further investigation. It also highlights screening-specific mechanisms of action that should be considered to further optimise intervention effectiveness. CI - (c) 2026. The Author(s). FAU - Legendre, Eva AU - Legendre E AD - Hopital Europeen, Marseille, France. eva.legendre25@hotmail.fr. FAU - Dutrey-Kaiser, Anne AU - Dutrey-Kaiser A AD - Prospective et Cooperation, Marseille, France. FAU - Attalah, Yazid AU - Attalah Y AD - Association Sante Environnement pour Tous (SEPT), Marseille, France. FAU - Boyer, Ghislaine AU - Boyer G AD - Prospective et Cooperation, Marseille, France. FAU - Nauleau, Steve AU - Nauleau S AD - Agence Regionale de Sante Provence-Alpes Cote d'Azur, Marseille, France. FAU - Gaudart, Jean AU - Gaudart J AD - Prospective et Cooperation, Marseille, France. AD - Aix Marseille Univ, IRD, INSERM, UMR1252, SESSTIM, APHM, ISSPAM, BioSTIC, Marseille, France. FAU - Kelly, David AU - Kelly D AD - Sante publique France Provence-Alpes-Cote d'Azur et Corse, Marseille, France. FAU - Caserio-Schonemann, Celine AU - Caserio-Schonemann C AD - Sante publique France Provence-Alpes-Cote d'Azur et Corse, Marseille, France. FAU - Malfait, Philippe AU - Malfait P AD - Sante publique France Provence-Alpes-Cote d'Azur et Corse, Marseille, France. FAU - Chaud, Pascal AU - Chaud P AD - Sante publique France Provence-Alpes-Cote d'Azur et Corse, Marseille, France. FAU - Ramalli, Lauriane AU - Ramalli L AD - Sante publique France Provence-Alpes-Cote d'Azur et Corse, Marseille, France. FAU - Gastaldi, Chrystelle AU - Gastaldi C AD - Agence Regionale de Sante Provence-Alpes Cote d'Azur, Marseille, France. FAU - Franke, Florian AU - Franke F AD - Sante publique France Provence-Alpes-Cote d'Azur et Corse, Marseille, France. FAU - Rebaudet, Stanislas AU - Rebaudet S AD - Hopital Europeen, Marseille, France. AD - SESSTIM, Aix Marseille Univ, IRD, INSERM, ISSPAM, Marseille, France. LA - eng GR - no. 23DIRA031-0/Sante publique France/ GR - INCa_19519/Institut National Du Cancer/ GR - no. C2022000/Agence Regionale de Sante Provence-Alpes Cote d'Azur/ PT - Journal Article DEP - 20260803 PL - England TA - BMC Public Health JT - BMC public health JID - 100968562 SB - IM MH - Humans MH - Cross-Sectional Studies MH - Female MH - Middle Aged MH - France MH - *Early Detection of Cancer/statistics & numerical data MH - Adult MH - Aged MH - Male MH - *Health Promotion/methods/organization & administration MH - Adolescent MH - Young Adult MH - Colorectal Neoplasms/diagnosis MH - Uterine Cervical Neoplasms/diagnosis MH - *Vulnerable Populations/statistics & numerical data MH - Breast Neoplasms/diagnosis MH - Health Knowledge, Attitudes, Practice MH - Program Evaluation OTO - NOTNLM OT - Breast cancer OT - Cancer screening OT - Cervical smear OT - Colorectal cancer OT - Faecal immunochemical test OT - HPV OT - Health mediation OT - Mammography OT - Patient navigation OT - Uterine cervical cancer COIS- Declarations. Ethics approval and consent to participate: The anonymised questionnaires were validated by the Data protection officer of Sante publique France (SpF) prior to the survey in accordance with the European General Data Protection Regulation. The study was approved by the Hopital Europeen ethics committee (No. 2025-08-05-13 S). Participants provided oral informed consent in accordance with French regulations. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:12 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/04/01 00:00 [received] PHST- 2026/07/21 00:00 [accepted] PHST- 2026/10/30 01:12 [entrez] AID - 10.1186/s12889-026-28710-5 [pii] AID - 10.1186/s12889-026-28710-5 [doi] PST - epublish SO - BMC Public Health. 2026 Aug 3;26(1):3129. doi: 10.1186/s12889-026-28710-5. PMID- 42958521 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1477-7819 (Electronic) IS - 1477-7819 (Linking) VI - 24 IP - 1 DP - 2026 Aug 3 TI - Clinical outcomes of first-line nivolumab plus chemotherapy in patients aged >/= 75 years with unresectable advanced or recurrent gastric cancer: a retrospective study. LID - 413 [pii] LID - 10.1186/s12957-026-04525-x [doi] AB - BACKGROUND: Older patients with unresectable advanced or recurrent gastric cancer remain underrepresented in clinical trials of immune checkpoint inhibitors. This study assessed the clinical feasibility of first-line nivolumab plus chemotherapy in patients aged >/= 75 years treated at our institutions. METHODS: This retrospective study included 82 patients with HER2-negative unresectable advanced or recurrent gastric cancer who received first-line nivolumab-containing chemotherapy between January 2022 and February 2025. Patients were divided into two groups: those aged < 75 years (n = 52) and those aged >/= 75 years (n = 30). The primary endpoint was progression-free survival (PFS); secondary endpoints included overall survival (OS), objective response rate (ORR), disease control rate (DCR), and adverse events (AEs). RESULTS: Patients aged >/= 75 years more frequently had comorbidities, hypertension, and initial dose reductions. Median follow-up was 27.7 months in the < 75 years group and 24.3 months in the >/= 75 years group. Median PFS was 7.2 months in the < 75 years group and 5.6 months in the >/= 75 years group (P = 0.779). Median OS was 12.5 and 13.3 months, respectively (P = 0.852). No significant differences were observed in ORR, DCR, or AEs between the two groups. CONCLUSIONS: These findings suggest that first-line nivolumab plus chemotherapy may represent a clinically feasible treatment option for selected patients aged >/= 75 years with HER2-negative, unresectable, advanced or recurrent gastric cancer. Nevertheless, given the limited sample size, these results should be interpreted with caution and should not be construed as evidence of equivalent efficacy or safety between age groups. CI - (c) 2026. The Author(s). FAU - Yoshimatsu, Masaki AU - Yoshimatsu M AUID- ORCID: 0009-0001-4863-7452 AD - Department of Upper Gastrointestinal Surgery, Dokkyo Medical University, 880 Kitakobayashi, Mibu, Tochigi, 321-0293, Japan. my-1157@dokkyomed.ac.jp. FAU - Morita, Shinji AU - Morita S AD - Department of Upper Gastrointestinal Surgery, Dokkyo Medical University, 880 Kitakobayashi, Mibu, Tochigi, 321-0293, Japan. FAU - Ueta, Yu AU - Ueta Y AD - Department of Upper Gastrointestinal Surgery, Dokkyo Medical University, 880 Kitakobayashi, Mibu, Tochigi, 321-0293, Japan. FAU - Mise, Minori AU - Mise M AD - Department of Surgery, Dokkyo Medical University Nikko Medical Center, Tochigi, Japan. FAU - Inoue, Noboru AU - Inoue N AD - Department of Surgery, Dokkyo Medical University Nikko Medical Center, Tochigi, Japan. FAU - Ochiai, Takahiro AU - Ochiai T AD - Department of Upper Gastrointestinal Surgery, Dokkyo Medical University, 880 Kitakobayashi, Mibu, Tochigi, 321-0293, Japan. FAU - Takise, Shuhei AU - Takise S AD - Department of Upper Gastrointestinal Surgery, Dokkyo Medical University, 880 Kitakobayashi, Mibu, Tochigi, 321-0293, Japan. FAU - Fujita, Junki AU - Fujita J AD - Department of Upper Gastrointestinal Surgery, Dokkyo Medical University, 880 Kitakobayashi, Mibu, Tochigi, 321-0293, Japan. FAU - Muroi, Hiroto AU - Muroi H AD - Department of Surgery, Dokkyo Medical University Nikko Medical Center, Tochigi, Japan. FAU - Nakagawa, Masatoshi AU - Nakagawa M AD - Department of Upper Gastrointestinal Surgery, Dokkyo Medical University, 880 Kitakobayashi, Mibu, Tochigi, 321-0293, Japan. FAU - Nakajima, Masanobu AU - Nakajima M AD - Department of Upper Gastrointestinal Surgery, Dokkyo Medical University, 880 Kitakobayashi, Mibu, Tochigi, 321-0293, Japan. FAU - Yamaguchi, Satoru AU - Yamaguchi S AD - Department of Surgery, Dokkyo Medical University Nikko Medical Center, Tochigi, Japan. FAU - Kojima, Kazuyuki AU - Kojima K AD - Department of Upper Gastrointestinal Surgery, Dokkyo Medical University, 880 Kitakobayashi, Mibu, Tochigi, 321-0293, Japan. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - World J Surg Oncol JT - World journal of surgical oncology JID - 101170544 RN - 31YO63LBSN (Nivolumab) SB - IM MH - Humans MH - *Stomach Neoplasms/drug therapy/pathology/mortality MH - *Nivolumab/administration & dosage/therapeutic use MH - Female MH - Retrospective Studies MH - Aged MH - *Neoplasm Recurrence, Local/drug therapy/pathology/mortality MH - *Antineoplastic Combined Chemotherapy Protocols/therapeutic use MH - Male MH - Aged, 80 and over MH - Prognosis MH - Survival Rate MH - Follow-Up Studies MH - Middle Aged MH - Progression-Free Survival MH - Age Factors OTO - NOTNLM OT - Aged OT - Immune checkpoint inhibitors OT - Nivolumab OT - Retrospective studies OT - Stomach neoplasms COIS- Declarations. Ethics approval and consent to participate: The study protocol was approved by the Institutional Review Board of Dokkyo Medical University (approval number: R-105-4 J). This study was conducted in accordance with the Declaration of Helsinki. The requirement for informed consent was waived owing to the retrospective nature of the study, and information about the study was disclosed using an opt-out approach. Consent for publication: Not applicable because this study does not contain any individually identifiable patient data. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:12 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/06/01 00:00 [received] PHST- 2026/07/29 00:00 [accepted] PHST- 2026/10/30 01:12 [entrez] AID - 10.1186/s12957-026-04525-x [pii] AID - 10.1186/s12957-026-04525-x [doi] PST - epublish SO - World J Surg Oncol. 2026 Aug 3;24(1):413. doi: 10.1186/s12957-026-04525-x. PMID- 42958516 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2105 (Electronic) IS - 1471-2105 (Linking) VI - 27 IP - 1 DP - 2026 Aug 3 TI - Extracting structure-activity relationships from patents with large language models. LID - 251 [pii] LID - 10.1186/s12859-026-06587-w [doi] AB - BACKGROUND: Persistent clinical failures in drug development arise from multiple factors, including imbalanced molecular properties, underscoring the need for systematic structure-activity relationship analysis to link structural modifications with optimized efficacy, selectivity, and safety of small-molecule drugs. Compared with academic literature, drug-related patent documents contain earlier and richer structure-activity relationship information that can support preclinical decision making. However, extracting structure-activity relationship information from patents is technically challenging due to ambiguous terminology, fragmented tables, dispersed compound descriptions, and the need to correctly link compound identities with activity endpoints. To address these issues, we developed PatSAR, a prompt-engineering-centered LLM workflow implemented through the ChatGPT web interface, in which finalized task-specific prompts guide document-by-document extraction of SAR information from patent PDF documents. RESULTS: When evaluated on 214 PARP-related patents containing 8226 expert-annotated SAR entities, PatSAR achieved a recall of 79.05%, a precision of 99.65%, and an F1 score of 88.16%. In a multi-target generalization test set of 200 recent patents across ten non-PARP small-molecule targets, PatSAR achieved a recall of 85.25%, a precision of 98.97%, and an F1 score of 91.60%. In an external comparison on DocSAR-Patent-96, PatSAR achieved a Table Recall of 81.86%, compared with the reported Doc2SAR result of 74.09% on the patent subset. CONCLUSIONS: These results support the potential of large language models for patent information extraction and highlight the role of prompt engineering in improving task-specific extraction performance. The proposed PatSAR framework illustrates the promise of hybrid human-AI workflows for SAR curation and patent analysis. CI - (c) 2026. The Author(s). FAU - Zheng, Shaolong AU - Zheng S AD - State Key Laboratory of Mechanism and Quality of Chinese Medicine, University of Macau, Room 1049, E12, Macao SAR, 999078, China. FAU - Yao, Gengyuan AU - Yao G AD - State Key Laboratory of Mechanism and Quality of Chinese Medicine, University of Macau, Room 1049, E12, Macao SAR, 999078, China. FAU - Cai, Hong AU - Cai H AD - State Key Laboratory of Mechanism and Quality of Chinese Medicine, University of Macau, Room 1049, E12, Macao SAR, 999078, China. FAU - Ma, Ningxuan AU - Ma N AD - Faculty of Computing, School of Computer Science and Technology (Shenzhen), Harbin Institute of Technology, Building L, HIT Campus, Shenzhen UniversityTown, Xili, Shenzhen, China. FAU - Zhang, Xiaofeng AU - Zhang X AD - Faculty of Computing, School of Computer Science and Technology (Shenzhen), Harbin Institute of Technology, Building L, HIT Campus, Shenzhen UniversityTown, Xili, Shenzhen, China. zhangxiaofeng@hit.edu.cn. FAU - Hu, Yuanjia AU - Hu Y AD - State Key Laboratory of Mechanism and Quality of Chinese Medicine, University of Macau, Room 1049, E12, Macao SAR, 999078, China. yuanjiahu@um.edu.mo. AD - Centre for Pharmaceutical Regulatory Sciences, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China. yuanjiahu@um.edu.mo. AD - DPM, Faculty of Health Sciences, University of Macau, Macao SAR, China. yuanjiahu@um.edu.mo. LA - eng GR - No.: 0002/2025/NRP, 0008/2025/EQP, and 0049/2024/AGJ/the Science and Technology Development Fund of Macau SAR/ GR - (No.: 0002/2025/NRP, 0008/2025/EQP, and 0049/2024/AGJ/the Science and Technology Development Fund of Macau SAR/ GR - No.: MYRG-CRG2023-00007-ICMS-IAS, MYRG-GRG2024-00268-ICMS-UMDF, UMDF-TISF/2025/011/ICMS, and SHMDF-AI/2026/003/the University of Macau, University of Macau Development Foundation (UMDF), and Dr. Stanley Ho Medical Development Foundation/ PT - Journal Article DEP - 20260803 PL - England TA - BMC Bioinformatics JT - BMC bioinformatics JID - 100965194 SB - IM MH - *Large Language Models MH - *Patents as Topic MH - Structure-Activity Relationship MH - *Data Mining/methods MH - Humans OTO - NOTNLM OT - Large language models OT - Lead optimization OT - Pharmaceutical patent OT - Prompt engineering OT - Structure-activity relationships COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:11 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/01/23 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/30 01:11 [entrez] AID - 10.1186/s12859-026-06587-w [pii] AID - 10.1186/s12859-026-06587-w [doi] PST - epublish SO - BMC Bioinformatics. 2026 Aug 3;27(1):251. doi: 10.1186/s12859-026-06587-w. PMID- 42958515 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-230X (Electronic) IS - 1471-230X (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - A fatty liver index-based sequential strategy for identifying hepatic steatosis in individuals with suspected steatotic liver disease. LID - 712 [pii] LID - 10.1186/s12876-026-05173-x [doi] AB - OBJECTIVE: Fatty liver index (FLI) is widely used as a non-invasive tool for identifying hepatic steatosis but performs poorly in lean individuals and leaves a substantial proportion of individuals within an indeterminate range. This cross-sectional study evaluated whether combination strategies could improve identification of hepatic steatosis in a selected referred population with suspected steatotic liver disease (SLD). METHODS: In a derivation cohort of 1648 subjects with suspected SLD who underwent MRI-PDFF assessment between January 2015 and January 2022, we evaluated five non-invasive indices (FLI, hepatic steatosis index [HSI], liver fat score [LFS], visceral adiposity index [VAI], and triglyceride-glucose index [TyG]). Four FLI-based sequential strategies were developed and further validated in an independent biopsy-based cohort of 426 patients from five tertiary centers in South China. RESULTS: FLI performed best in identifying hepatic steatosis among the five non-invasive indices, with an AUROC of 0.75, but showed a high missed-identification rate exceeding 90% in individuals with BMI < 23 kg/m(2). In Strategy 4, the FLI-LFS sequential algorithm improved identification performance in lean individuals. In the derivation cohort, sensitivity increased from 9.4% to 78.6%, the missed-identification rate decreased from 90.6% to 21.4%, and accuracy increased from 30.0% to 73.1%. A similar improvement in missed identification was observed in the validation cohort. CONCLUSIONS: Compared with FLI alone, the sequential FLI-LFS algorithm substantially improved the identification of hepatic steatosis among lean individuals with suspected SLD. The strategy may help identify individuals who require further imaging and etiological evaluation but should not be regarded as a stand-alone tool for determining the subtype or cause of SLD. CI - (c) 2026. The Author(s). FAU - Yang, Yan AU - Yang Y AD - Department of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan II Road, Yuexiu District, Guangzhou, 510000, Guangdong, China. FAU - Feng, Xiongcai AU - Feng X AD - Department of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan II Road, Yuexiu District, Guangzhou, 510000, Guangdong, China. FAU - Deng, Hong AU - Deng H AD - Department of Infectious Diseases, The Third Affiliated Hospital, Sun Yat-sen University, No.600 Tianhe Road, Tianhe District, Guangzhou, 510080, China. FAU - Dong, Zhiyong AU - Dong Z AD - Department of Bariatric Surgery, The First Affiliated Hospital, Jinan University, No. 613 West Huangpu Avenue, Tianhe District, Guangzhou, 510080, China. FAU - Xu, Lishu AU - Xu L AD - Department of Geriatrics, Guangdong Provincial People's Hospital, No. 106 Zhongshan II Road, Yuexiu District, Guangzhou, 510000, Guangdong, China. FAU - Feng, Shiting AU - Feng S AD - Department of Radiology, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan II Road, Yuexiu District, Guangzhou, 510000, Guangdong, China. FAU - Liao, Bing AU - Liao B AD - Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan II Road, Yuexiu District, Guangzhou, 510000, Guangdong, China. FAU - Shao, Congxiang AU - Shao C AD - Department of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan II Road, Yuexiu District, Guangzhou, 510000, Guangdong, China. FAU - Feng, Pinning AU - Feng P AD - Department of Clinical Laboratory, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan II Road, Yuexiu District, Guangzhou, China. fengpn@mail.sysu.edu.cn. FAU - Zhong, Bihui AU - Zhong B AD - Department of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan II Road, Yuexiu District, Guangzhou, 510000, Guangdong, China. zhongbh@mail.sysu.edu.cn. FAU - Ye, Junzhao AU - Ye J AD - Department of Gastroenterology, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan II Road, Yuexiu District, Guangzhou, 510000, Guangdong, China. yejzh@mail2.sysu.edu.cn. LA - eng GR - 82400663/National Natural Science Foundation of China/ GR - 82370587/National Natural Science Foundation of China/ GR - 82100648/National Natural Science Foundation of China/ PT - Journal Article PT - Multicenter Study DEP - 20260803 PL - England TA - BMC Gastroenterol JT - BMC gastroenterology JID - 100968547 RN - 0 (Triglycerides) SB - IM MH - Humans MH - Female MH - Cross-Sectional Studies MH - Male MH - Middle Aged MH - *Fatty Liver/diagnosis/pathology/diagnostic imaging MH - Magnetic Resonance Imaging MH - Adult MH - Algorithms MH - Liver/pathology/diagnostic imaging MH - Triglycerides/blood MH - Sensitivity and Specificity MH - Body Mass Index MH - *Non-alcoholic Fatty Liver Disease/diagnosis MH - Severity of Illness Index MH - Intra-Abdominal Fat/diagnostic imaging MH - Biopsy OTO - NOTNLM OT - Fatty liver index OT - Hepatic steatosis OT - Lean individuals OT - Liver fat score OT - Steatotic liver disease COIS- Declarations. Ethics approval and consent to participate: This study performed in accordance with the Declaration of Helsinki and have approved by the Clinical Research Ethics Committee of the First Affiliated Hospital of Sun Yat-sen University (ethical number: [2020]187). Consent for publication: Not applicable. Competing interest: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:11 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/04 00:00 [received] PHST- 2026/07/23 00:00 [accepted] PHST- 2026/10/30 01:11 [entrez] AID - 10.1186/s12876-026-05173-x [pii] AID - 10.1186/s12876-026-05173-x [doi] PST - epublish SO - BMC Gastroenterol. 2026 Aug 3;26(1):712. doi: 10.1186/s12876-026-05173-x. PMID- 42958511 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1477-3155 (Electronic) IS - 1477-3155 (Linking) VI - 24 IP - 1 DP - 2026 Aug 1 TI - Bletilla striata-derived extracellular vesicles armed probiotic by supramolecular assembly ameliorates colitis via macrophage modulation and microbiota restoration. LID - 1000 [pii] LID - 10.1186/s12951-026-04866-5 [doi] AB - BACKGROUND: Inflammatory bowel disease (IBD) represents major global health challenges, but current treatments often are limited by efficacy loss, infection risks, and disruption of gut homeostasis. Novel therapies are needed to actively promote mucosal repair and restore immune balance. RESULTS: This study develops a nano-biotherapeutic by conjugating extracellular vesicles derived from Bletilla striata (BEVs) with the probiotic Escherichia coli Nissle 1917 (EcN) via supramolecular assembly. BEVs contained protocatechualdehyde as a key bioactive metabolite to suppress M1 macrophage polarization and reactive oxygen species (ROS) production. EcN@BEVs treatment significantly restored epithelial barrier integrity with the upregulated expression of ZO-1 and Occludin, and reversed microbiota dysbiosis by increasing the Firmicutes/Bacteroidota ratio. single-cell RNA sequencing (scRNA-seq) and invasion-adhesion-directed expression sequencing (INVADE-seq) analyses confirmed that EcN@BEVs promoted a shift from pro-inflammatory to anti-inflammatory macrophage phenotypes and reduced intracellular bacterial invasion. CONCLUSIONS: The EcN@BEVs complex demonstrates significant therapeutic potential for colitis through a multi-faceted mechanism involving enhanced probiotic delivery, direct anti-inflammatory action, gut microbiota restoration, and mucosal immunity reprogramming. This study presents a promising integrated nanotherapeutic strategy for IBD treatment under cross-species collaboration. CI - (c) 2026. The Author(s). FAU - Hou, Weiliang AU - Hou W AD - Department of Gastroenterology, Shanghai Institute of Pancreatic Diseases, Shanghai Key Laboratory of Nautical Medicine and Translation of Drugs and Medical Devices, National Key Laboratory of Immunity and Inflammation, Changhai Clinical Research Unit, Changhai Hospital, Naval Medical University, Shanghai, 200433, China. FAU - Sheng, Kaiwen AU - Sheng K AD - Department of Colorectal Surgery, The First Affiliated Hospital (Changhai Hospital), Naval Medical University, Shanghai, 200433, China. FAU - Gao, Minxin AU - Gao M AD - Department of Colorectal Surgery, The First Affiliated Hospital (Changhai Hospital), Naval Medical University, Shanghai, 200433, China. FAU - Liu, Bing AU - Liu B AD - Department of Colorectal Surgery, The First Affiliated Hospital (Changhai Hospital), Naval Medical University, Shanghai, 200433, China. FAU - Wang, Hao AU - Wang H AD - Department of Colorectal Surgery, The First Affiliated Hospital (Changhai Hospital), Naval Medical University, Shanghai, 200433, China. wanghaohh@vip.126.com. FAU - Li, Xu AU - Li X AD - Department of Colorectal Surgery, The First Affiliated Hospital (Changhai Hospital), Naval Medical University, Shanghai, 200433, China. xuli_ch@163.com. FAU - Shi, Yunjie AU - Shi Y AD - Department of Colorectal Surgery, The First Affiliated Hospital (Changhai Hospital), Naval Medical University, Shanghai, 200433, China. shiyunpengjie@126.com. LA - eng GR - 2025M784489/China Postdoctoral Science Foundation/ PT - Journal Article DEP - 20260801 PL - England TA - J Nanobiotechnology JT - Journal of nanobiotechnology JID - 101152208 RN - 0 (Reactive Oxygen Species) SB - IM MH - *Probiotics/pharmacology/chemistry MH - Animals MH - *Macrophages/drug effects/metabolism MH - *Colitis/drug therapy MH - Mice MH - *Extracellular Vesicles/chemistry/metabolism MH - *Orchidaceae/chemistry MH - Escherichia coli MH - *Gastrointestinal Microbiome/drug effects MH - Mice, Inbred C57BL MH - Humans MH - Reactive Oxygen Species/metabolism OTO - NOTNLM OT - Bletilla striata OT - Colitis OT - Macrophage polarization OT - Probiotic delivery OT - Supramolecular assembly COIS- Declarations. Ethics approval and consent to participate: This study was approved by the Ethics Committee of the Naval Medical University (Reference: NMUMREC-2021-005, Research Proposal No.: 20210310005, Conference Date: March 10, 2021) per the Declaration of Helsinki. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:11 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/06 00:00 [received] PHST- 2026/07/24 00:00 [accepted] PHST- 2026/10/30 01:11 [entrez] AID - 10.1186/s12951-026-04866-5 [pii] AID - 10.1186/s12951-026-04866-5 [doi] PST - epublish SO - J Nanobiotechnology. 2026 Aug 1;24(1):1000. doi: 10.1186/s12951-026-04866-5. PMID- 42958507 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1479-5876 (Electronic) IS - 1479-5876 (Linking) VI - 24 IP - 1 DP - 2026 Jul 31 TI - Targeting tetrahydrobiopterin metabolism in diabetic neuropathic pain: mechanisms, implications, and therapeutic strategies. LID - 1365 [pii] LID - 10.1186/s12967-026-08733-7 [doi] AB - BACKGROUND: Diabetic neuropathic pain (DNP) is a debilitating complication affecting up to 50% of diabetic patients and severely impairing their quality of life. Current treatments provide limited relief and carry considerable side effects, underscoring the urgent need for novel, mechanism-based analgesics. Recent studies have identified tetrahydrobiopterin (BH4) as a crucial modulator of pain sensitivity. After nerve injury, its levels surge in injured neurons and infiltrating immune cells, amplifying pain signals via nitric oxide (NO) production, neuroinflammation, and ion channel sensitisation. MAIN TEXT: This review addresses the critical translational challenge posed by the BH4 paradox in DNP: although systemic BH4 is essential for vascular and metabolic integrity and is depleted under chronic hyperglycaemia, its localised overproduction in the nervous system drives neuropathic hypersensitivity. Non-selective inhibition of the key upstream enzyme, GTP cyclohydrolase-1 (GCH1), risks systemic BH4 deficiency, impairing neurotransmitter biosynthesis and causing severe vascular and metabolic side effects. Attention has therefore turned to sepiapterin reductase (SPR), the terminal enzyme in BH4 de novo biosynthesis, as a more selective and safer target. SPR inhibition suppresses pathological BH4 overproduction in nociceptive and immune tissues while preserving physiological levels through the dihydrofolate reductase (DHFR)-mediated salvage pathway. Preclinical studies of SPR inhibitors such as sulfasalazine show analgesia with limited central nervous system side effects due to their restricted blood-brain barrier (BBB) permeability. To guide clinical application, we synthesise the biochemical, genetic, and physiological roles of BH4 in DNP, and propose a translational biomarker framework for monitoring target engagement and systemic safety in future clinical trials. CONCLUSION: We outline the mechanistic rationale and translational considerations for developing highly selective SPR inhibitors as a potentially better-tolerated, more selective approach for DNP and other BH4-driven chronic pain conditions. This rationale currently rests largely on genetic, preclinical, and surrogate-biomarker evidence, and no completed human trial has evaluated SPR inhibition for chronic pain. The proposed biomarker framework is therefore intended to inform trial design and dose optimisation rather than to imply current clinical readiness, as one step toward a future precision-medicine strategy for pain. CI - (c) 2026. The Author(s). FAU - Wei, Zhaoyinling AU - Wei Z AD - Department of Endocrinology, Renmin Hospital of Wuhan University, Jiefang RD #238, Wuhan, 430060, China. FAU - Amarthya, Dey AU - Amarthya D AD - Department of Endocrinology, Renmin Hospital of Wuhan University, Jiefang RD #238, Wuhan, 430060, China. FAU - Tesfaye, Solomon AU - Tesfaye S AD - Sheffield Teaching Hospitals and the University of Sheffield, Sheffield, UK. FAU - Gao, Ling AU - Gao L AUID- ORCID: 0000-0002-5033-0433 AD - Department of Endocrinology, Renmin Hospital of Wuhan University, Jiefang RD #238, Wuhan, 430060, China. ling.gao@whu.edu.cn. LA - eng GR - 82270861/National Natural Science Foundation of China/ GR - 2042020kf1079/Fundamental Research Funds for the Central Universities/ PT - Journal Article PT - Review DEP - 20260731 PL - England TA - J Transl Med JT - Journal of translational medicine JID - 101190741 RN - 0 (Biopterins) RN - EGX657432I (sapropterin) RN - EC 3.5.4.16 (GTP Cyclohydrolase) RN - EC 1.1.1.153 (sepiapterin reductase) RN - EC 1.1.- (Alcohol Oxidoreductases) SB - IM MH - *Biopterins/analogs & derivatives/metabolism MH - Humans MH - *Diabetic Neuropathies/metabolism/therapy/drug therapy MH - Animals MH - *Neuralgia/metabolism/therapy/drug therapy MH - GTP Cyclohydrolase/metabolism MH - *Molecular Targeted Therapy MH - Alcohol Oxidoreductases OTO - NOTNLM OT - Diabetic neuropathy OT - Guanosine-triphosphate-cyclohydrolase-1 (GCH1) OT - Neuropathic pain OT - Sepiapterin reductase (SPR) inhibition OT - Targeted pain management OT - Tetrahydrobiopterin (BH4) metabolism COIS- Declarations. Ethics approval and consent to participate: Not applicable. Declaration of generative AI and AI-assisted technologies in the writing process: During the preparation of this work, the authors used ChatGPT-4 to improve the readability and language of the manuscript. The AI tool was not involved in the scientific decision-making, data analysis, or the generation of any scientific conclusions. The authors have reviewed the final text and assume full responsibility for the accuracy and integrity of the manuscript. Consent for publication: Not applicable. Competing interests: The authors declare that there are no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:11 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/12/06 00:00 [received] PHST- 2026/07/25 00:00 [accepted] PHST- 2026/10/30 01:11 [entrez] AID - 10.1186/s12967-026-08733-7 [pii] AID - 10.1186/s12967-026-08733-7 [doi] PST - epublish SO - J Transl Med. 2026 Jul 31;24(1):1365. doi: 10.1186/s12967-026-08733-7. PMID- 42958500 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2466 (Electronic) IS - 1471-2466 (Linking) VI - 26 IP - 1 DP - 2026 Aug 4 TI - Association between platelet-to-white blood cell ratio trajectories and all-cause mortality in critically ill patients with acute exacerbation of chronic obstructive pulmonary disease: a retrospective cohort study. LID - 513 [pii] LID - 10.1186/s12890-026-04551-1 [doi] AB - BACKGROUND: AECOPD is a leading cause of ICU admission and death in patients with COPD, yet readily accessible biomarkers for dynamic risk stratification remain scarce. The platelet-to-white blood cell ratio (PWR) is a promising inflammatory marker reflecting inflammatory burden and regenerative capacity. However, no studies have investigated PWR in AECOPD, and single-time-point measurements in critical illness are inherently limited by rapid physiological fluctuations. Whether longitudinal PWR trajectories outperform static measurements or conventional severity scores in AECOPD remains unknown. Accordingly, we investigated the association between dynamic PWR trajectories and all-cause mortality in this critically ill population. METHODS: Data were extracted from the Medical Information Mart for Intensive Care (MIMIC)-IV database. This retrospective cohort study enrolled critically ill patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Group-based trajectory modeling (GBTM) was used to identify distinct platelet-to-white blood cell ratio (PWR) trajectories; model selection was based on the Bayesian information criterion (BIC), average posterior probability (AvePP), odds of correct classification (OCC), and clinical interpretability. The primary outcome was 28-day all-cause mortality, with secondary outcomes including in-ICU, in-hospital, 90-day, and 365-day mortality. Multivariable Cox and logistic regression models were constructed to evaluate independent associations. Receiver operating characteristic (ROC) curves with the DeLong test were used to compare predictive performance. Subgroup and sensitivity analyses were performed to assess robustness, and restricted cubic spline (RCS) analysis was conducted to evaluate nonlinearity. RESULTS: Among 1,056 AECOPD patients, five distinct PWR trajectories were identified: Traj 1 (persistently low, n = 210), Traj 2 (low-to-moderate increasing, n = 333), Traj 3 (moderate-to-high increasing, n = 269), Traj 4 (moderate-to-low decreasing, n = 98), and Traj 5 (persistently high, n = 146). The primary findings demonstrated that compared with Trajectory 1, Trajectories 2, 3, and 5 were associated with significantly reduced risks of 28-day all-cause mortality, with adjusted hazard ratios (HRs) of 0.35 (95% confidence interval [CI]: 0.23-0.53), 0.45 (95% CI: 0.29-0.70), and 0.23 (95% CI: 0.12-0.46), respectively. In contrast, Trajectory 4 exhibited a comparable risk of 28-day mortality relative to Trajectory 1 (HR = 0.72, 95% CI: 0.43-1.20). Consistent patterns were observed for secondary outcomes, including the risks of in-hospital mortality, ICU mortality, 90-day mortality, and 365-day mortality. The PWR trajectory model (AUC = 0.661) outperformed single PWR (AUC = 0.596, P = 0.007) and was comparable to SOFA (AUC = 0.607, P = 0.051); their combination yielded the highest AUC (0.674, P = 0.002 vs. SOFA). CONCLUSION: In critically ill patients with AECOPD, PWR trajectories are independently associated with short- and long-term mortality. PWR trajectories offer superior prognostic performance to single PWR, comparable performance to SOFA, and significant incremental value when combined. CI - (c) 2026. The Author(s). FAU - Xie, Bo AU - Xie B AD - Department of Cardiology, Chengdu Integrated TCM & Western Medicine hospital, Chengdu, China. FAU - Teng, Hong AU - Teng H AD - Department of Pulmonary and Critical Care Medicine, School of Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China. FAU - He, Ji-Yun AU - He JY AD - Department of Cardiology, Chengdu Integrated TCM & Western Medicine hospital, Chengdu, China. FAU - Guo, Lu AU - Guo L AD - Department of Pulmonary and Critical Care Medicine, School of Medicine, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China. guolu@med.uestc.edu.cn. FAU - Zhang, Yi-Fan AU - Zhang YF AD - Department of Pulmonary and Critical Care Medicine, Chengdu Integrated TCM & Western Medicine Hospital, Chengdu, China. bmbetty116@hotmail.com. FAU - Cui, Xiao-Jiao AU - Cui XJ AD - Department of Pharmacy, Personalized Drug Research and Therapy Key Laboratory of Sichuan Province, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China. cuixiaojiao@med.uestc.edu.cn. LA - eng PT - Journal Article DEP - 20260804 PL - England TA - BMC Pulm Med JT - BMC pulmonary medicine JID - 100968563 RN - 0 (Biomarkers) SB - IM MH - Humans MH - *Pulmonary Disease, Chronic Obstructive/mortality/blood MH - Retrospective Studies MH - *Critical Illness/mortality MH - Female MH - Male MH - Aged MH - Leukocyte Count MH - Disease Progression MH - Platelet Count MH - Biomarkers/blood MH - Middle Aged MH - Severity of Illness Index MH - *Blood Platelets OTO - NOTNLM OT - Acute exacerbation of chronic obstructive pulmonary disease OT - Intensive care unit OT - Platelet-to-white blood cell ratio trajectory OT - Predictive performance OT - Risk stratification COIS- Declarations. Ethics approval and consent to participate: The Institutional Review Board at the Beth Israel Deaconess Medical Center granted a waiver of informed consent and approved the sharing of the research resource. Bo Xie and Xiao-Jiao Cui have passed the online training courses and exams (certification numbers: 74618521 and 59921922). This study was conducted in accordance with the Helsinki Declaration. All authors have read and approved the final manuscript. Consent for publication: Not required as the manuscript does not contain individuals' data. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:11 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/26 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/30 01:11 [entrez] AID - 10.1186/s12890-026-04551-1 [pii] AID - 10.1186/s12890-026-04551-1 [doi] PST - epublish SO - BMC Pulm Med. 2026 Aug 4;26(1):513. doi: 10.1186/s12890-026-04551-1. PMID- 42958499 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2458 (Electronic) IS - 1471-2458 (Linking) VI - 26 IP - 1 DP - 2026 Aug 4 TI - Multidimensional dietary patterns and cardiometabolic comorbidity in adults with anxiety disorders: evidence from NHANES and linked dietary databases using interpretable machine learning. LID - 3132 [pii] LID - 10.1186/s12889-026-28298-w [doi] AB - BACKGROUND: Anxiety disorders frequently co-occur with cardiometabolic conditions, yet little is known about how everyday dietary exposures shape cardiovascular vulnerability within this population. Existing studies typically focus on isolated nutrients or single dietary indices, overlooking the broader food environment in which dietary choices are made. This study examined how multiple dimensions of diet including micronutrient intake, dietary patterns and specific food items relate to hypertension-coronary heart disease (CHD) comorbidity among adults with anxiety disorders. METHODS: We analyzed data from seven NHANES cycles (2005-2018) to identify adults with anxiety disorders and assess the presence of hypertension-CHD comorbidity. Dietary exposures were evaluated using 24-hour recalls and aggregated across three complementary domains linked to NHANES: core micronutrients, MyPyramid Equivalents for food groups, and WWEIA for individual foods. Multicollinearity was assessed through correlation analysis, followed by feature selection using the Boruta algorithm. Class imbalance was addressed with the Synthetic Minority Over-sampling Technique (SMOTE). Six machine learning models were developed and compared. Feature-level associations between dietary micronutrients, daily dietary intake, and food consumption and the risk of hypertension-CHD comorbidity were interpreted using SHapley Additive exPlanations (SHAP) and Local Interpretable Model-Agnostic Explanations (LIME). Model stability was further evaluated through time-series analyses. RESULTS: Across analytic approaches, several consistent dietary signatures emerged. Higher intakes of vitamin E, polyunsaturated fatty acids, folate, calcium, protein, and total energy were associated with lower odds of comorbidity, whereas zinc and iron intake showed positive associations. Dietary patterns rich in protein foods, poultry, seafood, and vegetables were inversely related to comorbidity, while starchy vegetables and cured meats were associated with increased risk. At the food level, bananas, coffee, cream substitutes, and tomato-based products were linked to lower risk, whereas yeast breads, margarine, cakes, and pies were linked to higher risk. Machine-learning models reinforced these findings and highlighted the cumulative influence of food choices rather than single nutrients. CONCLUSIONS: In adults with anxiety disorders, we identified clear and consistent dietary patterns associated with the coexistence of hypertension and coronary heart disease. By combining micronutrient intake, dietary patterns, and food-level data with interpretable machine-learning approaches, this study demonstrates that diet may contribute to cardiometabolic vulnerability in this understudied population. These findings highlight diet as a modifiable factor that could be incorporated into broader prevention efforts and support the value of routine dietary assessment in both psychiatric and general healthcare settings. CI - (c) 2026. The Author(s). FAU - Yang, Qiaoxia AU - Yang Q AD - Institute of Cardiovascular Diseases, Xiamen Cardiovascular Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, 361006, China. AD - Department of Cardiology, Xiamen Cardiovascular Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, 361006, China. FAU - Lin, Qiuxing AU - Lin Q AD - Department of Cerebrovascular Disease, Fujian Medical University Union Hospital, Fuzhou, China. FAU - He, Yanli AU - He Y AD - School of Pharmaceutical Sciences, Xiamen University, Fujian, 361103, China. FAU - Wang, Yan AU - Wang Y AD - Institute of Cardiovascular Diseases, Xiamen Cardiovascular Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, 361006, China. wy@medmail.com.cn. AD - Department of Cardiology, Xiamen Cardiovascular Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, 361006, China. wy@medmail.com.cn. FAU - Nie, Xiaoqing AU - Nie X AD - Institute of Cardiovascular Diseases, Xiamen Cardiovascular Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, 361006, China. niexiaoqing@xmu.edu.cn. LA - eng GR - (2024GZL-GG28/Natural Science Foundation of Fujian Province/ GR - 2024J011431/Health high quality development technology plan project of Health Commission of Xiamen/ PT - Journal Article DEP - 20260804 PL - England TA - BMC Public Health JT - BMC public health JID - 100968562 SB - IM MH - Humans MH - Nutrition Surveys MH - Male MH - Female MH - *Diet/statistics & numerical data MH - Comorbidity MH - Adult MH - *Hypertension/epidemiology MH - *Anxiety Disorders/epidemiology MH - *Machine Learning MH - Middle Aged MH - *Coronary Disease/epidemiology MH - Databases, Factual MH - United States/epidemiology OTO - NOTNLM OT - Anxiety disorders OT - Comorbidity OT - Coronary heart disease OT - Dietary patterns OT - Hypertension OT - Micronutrients OT - Public health nutrition COIS- Declarations. Ethics approval and consent to participate: The National Health and Nutrition Examination Survey (NHANES) protocols were reviewed and approved by the National Center for Health Statistics (NCHS) Research Ethics Review Board. The survey was conducted in accordance with the ethical principles of the Declaration of Helsinki. All participants provided written informed consent prior to participation. Data used in this study were de-identified and publicly available; therefore, no additional ethical approval or informed consent was required for this secondary analysis. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:10 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/01/20 00:00 [received] PHST- 2026/06/19 00:00 [accepted] PHST- 2026/10/30 01:10 [entrez] AID - 10.1186/s12889-026-28298-w [pii] AID - 10.1186/s12889-026-28298-w [doi] PST - epublish SO - BMC Public Health. 2026 Aug 4;26(1):3132. doi: 10.1186/s12889-026-28298-w. PMID- 42958497 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2474 (Electronic) IS - 1471-2474 (Linking) VI - 27 IP - 1 DP - 2026 Aug 4 TI - Association of uric acid, lipid and bone metabolic markers with temporomandibular joint osteoarthritis: a cross-sectional study. LID - 936 [pii] LID - 10.1186/s12891-026-10300-6 [doi] AB - BACKGROUND: Temporomandibular joint osteoarthritis (TMJOA) is a degenerative disease with a complex and multifaceted etiology. Although metabolic disturbances have been linked to osteoarthritis in other joints, their role in TMJOA remains unclear. This study aimed to investigate the association between systemic metabolic indicators and TMJOA. METHODS: This cross-sectional study included 273 participants recruited from a tertiary temporomandibular joint (TMJ) clinic. Diagnosis was based on cone beam computed tomography (CBCT) findings and clinical criteria. Blood samples were analyzed for metabolic markers, including serum uric acid, lipid profiles, and bone turnover markers such as procollagen type 1 N-terminal propeptide (P1NP). Multivariable logistic regression analysis was performed to evaluate associations between metabolic indicators and TMJOA. Model selection was conducted using the Akaike information criterion. Restricted cubic spline (RCS) analysis was applied to explore potential nonlinear relationships. Subgroup analyses were performed according to age. Discriminatory ability was assessed using receiver operating characteristic (ROC) curve analysis. Least Absolute Shrinkage and Selection Operator (LASSO) regression was conducted to select indicators as sensitivity analysis. RESULTS: After adjusting for potential confounders, multivariable logistic regression revealed that serum uric acid was positively associated with the presence of TMJOA, whereas high-density lipoprotein cholesterol (HDL-C) and P1NP were identified as inverse associated factors (p < 0.05). LASSO regression also selected these three predictors and confirmed these findings. The combined panel of these systemic markers provided moderate discriminatory ability for TMJOA occurrence (AUC = 0.716), whereas individual markers showed limited predictive discrimination. RCS analysis suggested a possible nonlinear, U-shaped association between uric acid levels and disease risk, with a wider confidence interval and less precise estimates at the upper tail. Exploratory age-stratified analyses indicated that vitamin D and bone turnover markers were associated with disease in adolescents but not in adults, reflecting the possible influence of systemic metabolic shifts over the lifespan. CONCLUSIONS: In this single-center cross-sectional sample, elevated serum uric acid and reduced HDL-C and P1NP were associated with TMJOA presence. These exploratory associations require validation in population-based cohorts before clinical application. CI - (c) 2026. The Author(s). FAU - Zhang, Chu-Han AU - Zhang CH AD - Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, 200011, China. FAU - Li, Min-Han AU - Li MH AD - Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, 200011, China. FAU - He, Jia-Ying AU - He JY AD - Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, 200011, China. FAU - Wang, Bian AU - Wang B AD - Department of Assisted Reproduction, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, Shanghai, Shanghai, 200011, China. FAU - Jin, Shan AU - Jin S AD - Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, 200011, China. FAU - Chen, Miao AU - Chen M AD - Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, 200011, China. FAU - Yu, Da-Hai AU - Yu DH AD - Department of Stomatology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China. FAU - Yang, Chi AU - Yang C AD - Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, 200011, China. yangchi1963@hotmail.com. FAU - Ma, Zhi-Gui AU - Ma ZG AD - Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Research Unit of Oral and Maxillofacial Regenerative Medicine, Chinese Academy of Medical Sciences, Shanghai, 200011, China. mazhigui2007@126.com. LA - eng GR - 2023YFC2509100/he National Key R&D Program of China/ GR - JYYQ016/Excellent Young Scholars of Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine/ GR - NCRC0202339/The Clinical Diseases, Shanghai Ninth People's Hospital/ GR - JFXM202505/Faculty Development Training Program, Shanghai Jiao Tong University School of Medicine/ GR - 1QYB24068/Shanghai Association of Higher Education/ GR - JYJX03202310, JYJX03202414/Faculty Level Teaching Medical Education Research Project, The Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine/ GR - 2025CRW31/the Special Fund for Research Wards of Shanghai Ninth People's Hospital/ GR - KQHX2024-05, KQHX2025-06/Horizontal Project of Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine/ PT - Journal Article DEP - 20260804 PL - England TA - BMC Musculoskelet Disord JT - BMC musculoskeletal disorders JID - 100968565 RN - 268B43MJ25 (Uric Acid) RN - 0 (Biomarkers) RN - 0 (Procollagen) RN - 0 (Peptide Fragments) RN - 0 (procollagen Type I N-terminal peptide) RN - 0 (Lipids) SB - IM MH - Humans MH - Cross-Sectional Studies MH - *Uric Acid/blood MH - Female MH - Biomarkers/blood MH - Male MH - *Osteoarthritis/blood/diagnostic imaging MH - Middle Aged MH - Adult MH - *Temporomandibular Joint Disorders/blood/diagnostic imaging MH - *Procollagen/blood MH - *Peptide Fragments/blood MH - *Lipids/blood MH - Cone-Beam Computed Tomography MH - Aged OTO - NOTNLM OT - Bone metabolism OT - Lipid metabolism OT - Temporomandibular joint osteoarthritis OT - Uric acid OT - Vitamin D COIS- Declarations. Ethics approval and consent to participate: This study was approved by the Human Research Ethics Committee of Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine (Approval No. SH9H-2020-T7-1) and conducted in accordance with the Declaration of Helsinki and the STROBE guidelines. Written informed consent was obtained from all participants or their legal guardians. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:10 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/02/21 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/30 01:10 [entrez] AID - 10.1186/s12891-026-10300-6 [pii] AID - 10.1186/s12891-026-10300-6 [doi] PST - epublish SO - BMC Musculoskelet Disord. 2026 Aug 4;27(1):936. doi: 10.1186/s12891-026-10300-6. PMID- 42958494 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2180 (Electronic) IS - 1471-2180 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Genomic insights into azole resistance in Aspergillus fumigatus: findings from global and South African clinical isolates. LID - 935 [pii] LID - 10.1186/s12866-026-05293-0 [doi] AB - Aspergillus fumigatus is a major opportunistic fungal pathogen and an important cause of invasive aspergillosis in immunocompromised patients. Azole antifungals remain central to treatment, but azole resistance is increasingly reported. Azole resistance in A. fumigatus is often linked to canonical cyp51A mechanisms, but many resistant isolates lack these mutations, suggesting additional contributions. This study analysed 12 newly sequenced azole-susceptible clinical isolates from KwaZulu-Natal, South Africa, together with publicly available clinical and environmental A. fumigatus genomes using a uniform AF293 reference-based workflow for variant calling, population structure correction and genome wide association studies (GWAS). A filtered SNP-alignment matrix was used to infer population structure and perform structure-aware genome-wide association testing with azole susceptibility status as a binary phenotype. Overall, 194 of 227 genomes met predefined quality thresholds, and 31 949 biallelic SNPs were tested using ten PCoA axes as covariates. After adjustment using SNP-derived PCoA covariates, 16 SNPs exceeded the Bonferroni threshold and 206 SNPs passed BH-FDR correction. The strongest association signals were distributed across multiple genomic regions rather than being restricted to a single canonical resistance locus, with candidate annotations linked to sterol and lipid metabolism, membrane transport, transcriptional regulation, cell-wall remodelling and stress response. Transporter-focused analysis showed that all 12 South African isolates had negative CDR1 and MFS transporter-balance scores, placing them among the most transporter-depleted susceptible backgrounds in the dataset. Phylogenetic reconstruction showed interspersed resistant and susceptible isolates, supporting repeated emergence or local amplification rather than expansion of a single resistant clone. Overall, these findings support a cautious model in which azole resistance-associated variation in A. fumigatus is distributed across multiple functional pathways within a structured population background. The South African isolates provide a local susceptible clinical baseline for future expanded surveillance.Keywords: Aspergillus fumigatus, Azole resistance, Genome Wide Association, SNPs. CI - (c) 2026. The Author(s). FAU - Mbamali, Siphiwengesihle Kuhle Silindile AU - Mbamali SKS AUID- ORCID: 0009-0000-3579-4558 AD - Department of Biotechnology and Food Science, Faculty of Applied Sciences, Durban University of Technology, Durban, South Africa. FAU - Mohlomi, Nikwando AU - Mohlomi N AUID- ORCID: 0000-0001-9532-3504 AD - Department of Biotechnology and Food Science, Faculty of Applied Sciences, Durban University of Technology, Durban, South Africa. FAU - van der Nest, Magriet AU - van der Nest M AUID- ORCID: 0000-0001-6914-8343 AD - Department of Biochemistry, Genetics and Microbiology, Faculty of Natural and Agricultural Sciences, Forestry and Agricultural Biotechnology Institute (FABI), BGM Department, University of Pretoria, Pretoria, South Africa. FAU - Mchunu, Nokuthula Peace AU - Mchunu NP AD - College of Agriculture, Engineering and Science, School of Life Science, University of KwaZulu-Natal, Durban, South Africa. NP.Mchunu@NRF.ac.za. AD - National Research Foundation, Meiring Naude Road, Brummeria, Pretoria, South Africa. NP.Mchunu@NRF.ac.za. FAU - Permaul, Kugen AU - Permaul K AUID- ORCID: 0000-0001-5032-3092 AD - Department of Biotechnology and Food Science, Faculty of Applied Sciences, Durban University of Technology, Durban, South Africa. kugen@dut.ac.za. LA - eng GR - NFSG240503217275/NRF BAAP Grant/ PT - Journal Article DEP - 20260803 PL - England TA - BMC Microbiol JT - BMC microbiology JID - 100966981 RN - 0 (Azoles) RN - 0 (Antifungal Agents) RN - 0 (Fungal Proteins) SB - IM MH - *Aspergillus fumigatus/genetics/drug effects/isolation & purification MH - *Drug Resistance, Fungal/genetics MH - South Africa MH - Polymorphism, Single Nucleotide MH - *Azoles/pharmacology MH - *Antifungal Agents/pharmacology MH - Humans MH - *Aspergillosis/microbiology MH - Microbial Sensitivity Tests MH - Genome, Fungal MH - Genome-Wide Association Study MH - Fungal Proteins/genetics MH - Genomics COIS- Declarations. Ethics approval and consent to participate: Not applicable. This study analysed Aspergillus fumigatus isolates as microbial cultures and whole-genome sequence data. No human participants were recruited, no human specimens were collected by the investigators, and no patient-level clinical data were accessed. Isolates were supplied as de-identified cultures from routine diagnostics and were labelled only by broad source type (clinical or environmental). Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:10 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2025/12/16 00:00 [received] PHST- 2026/06/08 00:00 [accepted] PHST- 2026/10/30 01:10 [entrez] AID - 10.1186/s12866-026-05293-0 [pii] AID - 10.1186/s12866-026-05293-0 [doi] PST - epublish SO - BMC Microbiol. 2026 Aug 3;26(1):935. doi: 10.1186/s12866-026-05293-0. PMID- 42958486 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2288 (Electronic) IS - 1471-2288 (Linking) VI - 26 IP - 1 DP - 2026 Jul 25 TI - Supporting high-quality enrollment decision-making in clinical research: development of a novel informed consent communication framework and curriculum. LID - 231 [pii] LID - 10.1186/s12874-026-02885-2 [doi] AB - BACKGROUND: In clinical trials, the process of informed consent is intended to support prospective participants in making enrollment decisions that are well informed and aligned with their values and preferences. Despite the importance of relationship building during the consent process, there is little structured guidance for researchers on how to effectively facilitate informed consent conversations with prospective participants. This project aimed to develop and implement a relationship-based communication framework for clinical researchers, with the ultimate goal of supporting prospective participants in making values-aligned decisions. METHODS: The ENgaging with Values to Inform research enrollment deciSIONs (ENVISION) study was launched to improve the process of research enrollment decision-making within the context of a multisite cystic fibrosis clinical trial. The ENVISION team sought input from individuals with cystic fibrosis, researchers, institutional review board leaders, and clinical research leaders to understand key issues in consent communication in the context of cystic fibrosis trials. A working group of the ENVISION team incorporated these viewpoints into the development of a consent communication framework, which was guided by consideration of elements of the Ottawa Decision Support Framework and incorporated established communication strategies. The communication framework and an accompanying training curriculum were finalized through iterative review with the working group and the full team. RESULTS: The development process resulted in a four-component, values-oriented communication framework, the RIVER framework: Relationship (forming a connection with the prospective participant), Information (sharing about the study), Values Exploration (eliciting and examining relevant values), and Resolution (confirming a values-aligned decision). Each component includes considerations to support prospective participants in making values-aligned decisions. RIVER is implemented through a training curriculum designed for research coordinators. The training curriculum consists of two 2-hour sessions that include didactic teaching, group discussion, and role-play scenarios, which provide an opportunity to practice real-world clinical research enrollment conversations. CONCLUSION: The RIVER framework offers a structured approach to communication during clinical research consent conversations that is designed to enhance voluntary, informed decisions. Ongoing evaluation of the framework and training curriculum will inform opportunities for future revisions, adaptations, and broader dissemination. CI - (c) 2026. The Authors. Parts of this work were authored by US Federal Government authors and are not under copyright protection in the US; foreign copyright protection may apply. FAU - Kraft, Stephanie A AU - Kraft SA AD - Department of Bioethics and Decision Sciences, Geisinger College of Health Sciences, 100 N. Academy Ave, Danville, PA, 17822, USA. skraft1@geisinger.edu. FAU - Porter, Kathryn M AU - Porter KM AD - Treuman Katz Center for Pediatric Bioethics and Palliative Care, Seattle Children's Research Institute, Seattle, WA, USA. FAU - Brickler, Abigail AU - Brickler A AD - Department of Bioethics and Decision Sciences, Geisinger College of Health Sciences, 100 N. Academy Ave, Danville, PA, 17822, USA. FAU - Mazuera, Sylvia AU - Mazuera S AD - Community member, Carrollton, TX, USA. FAU - Taylor, Holly A AU - Taylor HA AD - Department of Bioethics, Clinical Center National Institutes of Health, Bethesda, MD, USA. FAU - Gelinas, Luke AU - Gelinas L AD - Advarra IRB, Columbia, MD, USA. FAU - Duenas, Devan M AU - Duenas DM AD - Treuman Katz Center for Pediatric Bioethics and Palliative Care, Seattle Children's Research Institute, Seattle, WA, USA. FAU - Tsing, Jennifer C AU - Tsing JC AD - Treuman Katz Center for Pediatric Bioethics and Palliative Care, Seattle Children's Research Institute, Seattle, WA, USA. FAU - George, Cynthia AU - George C AD - Cystic Fibrosis Foundation, Bethesda, MD, USA. FAU - Krishnamurti, Tamar AU - Krishnamurti T AD - Division of General Internal Medicine, University of Pittsburgh, Pittsburgh, PA, USA. FAU - Langford, Aisha T AU - Langford AT AD - Department of Family Medicine and Public Health Sciences, Wayne State University, Detroit, MI, USA. FAU - Mueller, Rebecca AU - Mueller R AD - Division of Translational Medicine and Human Genetics, Department of Medicine, University of Pennsylvania, Philadelphia, PA, USA. FAU - Ramos, Kathleen J AU - Ramos KJ AD - Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Medicine, University of Washington, Seattle, WA, USA. FAU - Vo, Holly H AU - Vo HH AD - Treuman Katz Center for Pediatric Bioethics and Palliative Care, Seattle Children's Research Institute, Seattle, WA, USA. FAU - Weiss, Elliott M AU - Weiss EM AD - Treuman Katz Center for Pediatric Bioethics and Palliative Care, Seattle Children's Research Institute, Seattle, WA, USA. FAU - Wilfond, Benjamin S AU - Wilfond BS AD - Department of Pediatrics, University of Washington, Seattle, WA, USA. CN - ENVISION Study Team LA - eng PT - Journal Article DEP - 20260725 PL - England TA - BMC Med Res Methodol JT - BMC medical research methodology JID - 100968545 SB - IM MH - Humans MH - *Informed Consent/standards MH - *Communication MH - Cystic Fibrosis/therapy MH - *Curriculum MH - *Decision Making MH - *Biomedical Research/methods MH - *Clinical Trials as Topic MH - Research Personnel/education MH - Prospective Studies MH - *Patient Selection/ethics OTO - NOTNLM OT - Clinical trials OT - Communication OT - Informed consent OT - Recruitment OT - Research relationships COIS- Declarations. Ethics approval and consent to participate: ENVISION development activities that occurred at Seattle Children's Research Institute were approved as exempt human subjects research by the Seattle Children's Research Institute IRB. Information including the project's goals, time required, and compensation was provided to all individuals engaged in the ENVISION process, but documentation of consent was not required. Activities that occurred at Geisinger were determined not to be human subjects research by the Geisinger IRB. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:10 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/02/04 00:00 [received] PHST- 2026/05/18 00:00 [accepted] PHST- 2026/10/30 01:10 [entrez] AID - 10.1186/s12874-026-02885-2 [pii] AID - 10.1186/s12874-026-02885-2 [doi] PST - epublish SO - BMC Med Res Methodol. 2026 Jul 25;26(1):231. doi: 10.1186/s12874-026-02885-2. PMID- 42958485 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2229 (Electronic) IS - 1471-2229 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Genome wide association mapping of soybean elite germplasm for spring-season adaptation by using high-density SNP array. LID - 1851 [pii] LID - 10.1186/s12870-026-09434-5 [doi] AB - Soybean (Glycine max L.) is a globally important crop and a major source of edible oil and plant-based protein; however, spring-season cultivation exposes soybean to elevated temperatures during critical reproductive stages, adversely affecting yield and seed quality. A panel of 100 advanced soybean accessions was screened across two variable planting dates of the spring season for two consecutive growing years, 2020 and 2021. Phenotypic data of six yield-related traits, i.e., plant height (PH), number of pods per plant (Pods), number of seeds per plant (Seeds), 100-seed weight (HSW), yield per plant (YPP), and days to maturity (DM) were collected. A genome-wide association study (GWAS) was conducted using the Fixed and Random Model Circulating Probability Unification (Farm-CPU) method by using 42,449 high-quality single-nucleotide polymorphisms (SNPs) from the SoySNP50K array. A total of 77 significant marker trait associations (MTAs) were identified. The highest number of MTAs is linked with plant height, and the lowest number of MTAs is linked with yield per plant. Genome-wide means of major allele frequency (MAF), genetic diversity (GD), and polymorphic information content (PIC) were 0.78, 0.28, and 0.28, respectively. Population structure and phylogenetic tree analysis were used to divide the whole population into two distinct groups. Furthermore, seven SNPs, i.e., ss715582919, ss715588087, ss715588504, ss715592876, ss715599604, ss715599732, and ss715599766, were associated with multiple traits across planting dates. Various clusters of significant SNPs were marked on chromosomes Gm08, Gm10, Gm17, Gm02, Gm12, Gm15, and Gm16 associated with various yield traits. Candidate gene analysis identified genes related to thermotolerance (HSP90, HSFA2), flowering time (FT2a), pod number (NAC29), and carbohydrate transport (SWEET15) within flanking regions of key SNPs. A comprehensive insight into the genetic architecture of soybean yield under spring-season conditions is a prerequisite for the development of climate-resilient soybean genotypes. These findings will contribute to devising future breeding strategies in soybean. CI - (c) 2026. The Author(s). FAU - Ghuman, Hasham Feroz AU - Ghuman HF AD - Center of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan. FAU - Habib, Madiha AU - Habib M AD - National Seed Research and Training Center, University of Agriculture, Faisalabad, Pakistan. FAU - Hussain, Shabbir AU - Hussain S AD - College of Resources and Environment, Shandong Agricultural University, 61 Daizong Street, Tai'an, Shandong Province, 271000, China. FAU - Ahmed, Zaheer AU - Ahmed Z AD - Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad, Pakistan. FAU - Murad, Muhammad AU - Murad M AD - Center of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan. FAU - Hameed, Kiran AU - Hameed K AD - National Seed Research and Training Center, University of Agriculture, Faisalabad, Pakistan. FAU - Awan, Bilal Ahmed AU - Awan BA AD - Institute of Horticultural Sciences, University of Agriculture, Faisalabad, Pakistan. FAU - Mubarik, Muhammad Salman AU - Mubarik MS AD - Department of Biotechnology, University of Narowal, Narowal, Pakistan. FAU - Awan, Faisal Saeed AU - Awan FS AD - Center of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, Pakistan. Faisal.saeed@uaf.edu.pk. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - BMC Plant Biol JT - BMC plant biology JID - 100967807 SB - IM MH - *Glycine max/genetics/physiology/growth & development MH - Genome-Wide Association Study MH - *Polymorphism, Single Nucleotide MH - Seasons MH - Chromosome Mapping MH - Phenotype MH - *Adaptation, Physiological/genetics MH - Genome, Plant MH - Quantitative Trait Loci OTO - NOTNLM OT - Genome-wide association study OT - Marker traits associations OT - Marker-assisted selections OT - Multi-trait associations OT - Spring-season adaptation OT - Yield traits COIS- Declarations. Ethics approval and consent to participate: Not applicable. Written informed consent was obtained from all authors for participation and publication. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:10 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/02/23 00:00 [received] PHST- 2026/06/30 00:00 [accepted] PHST- 2026/10/30 01:10 [entrez] AID - 10.1186/s12870-026-09434-5 [pii] AID - 10.1186/s12870-026-09434-5 [doi] PST - epublish SO - BMC Plant Biol. 2026 Aug 3;26(1):1851. doi: 10.1186/s12870-026-09434-5. PMID- 42958483 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2377 (Electronic) IS - 1471-2377 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Risk factors of recurrent hemorrhage following minimally invasive transcranial puncture and drainage in patients with spontaneous intracerebral hemorrhage: a retrospective cohort study. LID - 683 [pii] LID - 10.1186/s12883-026-05237-1 [doi] AB - OBJECTIVE: To explore the risk factors for recurrent hemorrhage in patients with spontaneous intracerebral hemorrhage (sICH) following minimally invasive transcranial puncture and drainage using a YL-1 hard-channel approach. METHODS: Clinical data, CT imaging data, and follow-up information for patients with sICH who were continuously admitted to the Department of Neurosurgery from January 2021 to June 2025 were extracted from our hospital information system. All patients underwent minimally invasive transcranial puncture and drainage using a YL-1 hard-channel approach under CT guidance. Patients were categorized according to the occurrence of recurrent hemorrhage following surgery. Statistical analyses were conducted on the patients' general clinical data, CT imaging data, and follow-up data. Univariate analysis and stepwise multivariate regression analysis were employed to identify relevant risk factors for postoperative recurrent hemorrhage (PRH). Additionally, the receiver operating characteristic curve (ROC) was utilized to further examine the independent risk factors and determine the cut-off value for predicting outcomes. RESULTS: A total of 159 patients was included in this study. Among them, 24 (15.1%) patients experienced recurrent hemorrhage after surgery. Univariate analysis indicated that a higher preoperative body mass index (BMI) (P = 0.049), a larger postoperative dosage of urokinase (UK) (P = 0.026), and a shorter time from onset to puncture (OPT) (P = 0.001) were statistically associated with PRH. Among the preoperative CT imaging parameters, irregular hematoma shape (P = 0.002) and the blend sign (P = 0.001) were also statistically correlated with PRH. Multivariate binary logistic stepwise regression analysis revealed that a shorter OPT (OR = 1.223, 95% CI = 1.080-1.386, P = 0.002) and irregular hematoma shape (OR = 7.110, 95% CI = 1.840-17.473, P = 0.004) were independent risk factors for PRH. Conversely, the blend sign was associated with a reduced risk for PRH. (OR = 0.073, 95% CI = 0.012-0.108, P < 0.001). Additionally, plotting the ROC curve for OPT yielded an area under the curve (AUC) of 0.840, with an exploratory threshold of 10.5 h, corresponding to a sensitivity of 0.958 and a specificity of 0.778. CONCLUSION: A shorter OPT and an irregular hematoma shape are independent risk factors for recurrent hemorrhage following minimally invasive transcranial puncture and drainage using a YL-1 hard-channel approach in patients with sICH. In contrast, the blend sign may be associated to a lower risk of PRH. And these findings warrant further validation through external studies. CI - (c) 2026. The Author(s). FAU - Shen, Yong AU - Shen Y AD - Department of Neurosurgery, Southern Central Hospital of Yunnan Province (The First People's Hospital of Honghe State), Gejiu, Yunnan, 661018, China. FAU - Li, Lin AU - Li L AD - Department of Neurosurgery, Southern Central Hospital of Yunnan Province (The First People's Hospital of Honghe State), Gejiu, Yunnan, 661018, China. FAU - Long, Wenjun AU - Long W AD - Department of Gynecology, Southern Central Hospital of Yunnan Province (The First People's Hospital of Honghe State), Gejiu, Yunnan, 661018, China. FAU - Shen, Lan AU - Shen L AD - Department of Emergency, Southern Central Hospital of Yunnan Province (The First People's Hospital of Honghe State), Gejiu, Yunnan, 661018, China. FAU - He, Ting AU - He T AD - Department of Outpatient, Southern Central Hospital of Yunnan Province (The First People's Hospital of Honghe State), Gejiu, Yunnan, 661018, China. FAU - Gan, Lijuan AU - Gan L AD - Department of Outpatient, Southern Central Hospital of Yunnan Province (The First People's Hospital of Honghe State), Gejiu, Yunnan, 661018, China. FAU - Zong, Xitao AU - Zong X AD - Department of Neurosurgery, Southern Central Hospital of Yunnan Province (The First People's Hospital of Honghe State), Gejiu, Yunnan, 661018, China. FAU - Tang, Jinwei AU - Tang J AD - Department of Neurosurgery, Southern Central Hospital of Yunnan Province (The First People's Hospital of Honghe State), Gejiu, Yunnan, 661018, China. FAU - Tang, Zhiwei AU - Tang Z AUID- ORCID: 0000-0001-9314-4465 AD - Department of Neurosurgery, First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650503, China. tangzhiwei7755@hotmail.com. FAU - Ding, Jiangbo AU - Ding J AUID- ORCID: 0000-0002-2172-5014 AD - Department of Neurosurgery, Southern Central Hospital of Yunnan Province (The First People's Hospital of Honghe State), Gejiu, Yunnan, 661018, China. MY1314521xhn@126.com. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - BMC Neurol JT - BMC neurology JID - 100968555 SB - IM MH - Humans MH - Male MH - Female MH - Risk Factors MH - *Cerebral Hemorrhage/surgery/diagnostic imaging MH - Middle Aged MH - Retrospective Studies MH - *Drainage/adverse effects/methods MH - Recurrence MH - Aged MH - *Punctures/adverse effects MH - *Minimally Invasive Surgical Procedures/adverse effects/methods MH - Tomography, X-Ray Computed MH - Adult OTO - NOTNLM OT - Minimally invasive puncture and drainage OT - Risk factors OT - Spontaneous intracerebral hemorrhage OT - recurrent hemorrhage COIS- Declarations. Ethics approval and consent to participate: This study was approved by the Medical Ethics Committee of Southern Central Hospital of Yunnan Province (The First People's Hospital of Honghe State), and the research process strictly complied with the ethical principles governing human medical research stipulated in the Declaration of Helsinki of the World Medical Association. Informed consent to participate was obtained from all participants or their legal representatives. Consent for publication: All patients with spontaneous intracerebral hemorrhage or their legal representatives signed the informed consent form. The consent to publicly publish identifiable data and figures was obtained from the patients or their legal representatives of the study. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:09 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/04/07 00:00 [received] PHST- 2026/07/30 00:00 [accepted] PHST- 2026/10/30 01:09 [entrez] AID - 10.1186/s12883-026-05237-1 [pii] AID - 10.1186/s12883-026-05237-1 [doi] PST - epublish SO - BMC Neurol. 2026 Aug 3;26(1):683. doi: 10.1186/s12883-026-05237-1. PMID- 42958479 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2229 (Electronic) IS - 1471-2229 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Genome-wide identification of the chalcone synthase (CHS) family in tigernut and function analysis of cechs57 in drought stress response. LID - 1855 [pii] LID - 10.1186/s12870-026-09629-w [doi] AB - BACKGROUND: Drought stress is a major abiotic constraint that severely impairs crop growth and reduces yield globally. Identification of drought-tolerant candidate genes and their application in molecular breeding represent effective strategies to alleviate drought-induced losses. METHODS: Genome-wide identification of CHS family members was performed based on conserved domain screening. Comprehensive bioinformatic analyses, including phylogenetic classification, conserved motif profiling, gene structure annotation and cis-acting, were conducted to characterize the CeCHS family. Transcriptome data were used to analyze expression patterns of CeCHSs under drought stress and screen candidate genes. Functional validation of the candidate gene CeCHS57 was carried out via heterologous expression in yeast and Arabidopsis thaliana. RESULTS: A total of 66 CeCHS genes were identified in the tigernut genome, which were phylogenetically classified into four distinct subfamilies. Members within the same subfamily shared highly conserved motif compositions, exon-intron structures and cis-element profiles. Subfamily B, the dominant clade of the CeCHS family, was abundantly enriched with drought-responsive regulatory elements including ABRE and MBS. CeCHS57 was selected as the core candidate gene based on its sustained high expression in both roots and leaves and strong drought responsiveness. Functional assays indicate that heterologous overexpression of CeCHS57 significantly improves drought tolerance in both yeast and transgenic Arabidopsis. Further physiological characterization supports a mechanism whereby CeCHS57 confers drought resistance through coordinated activation of enzymatic and non-enzymatic antioxidant systems, enhanced reactive oxygen species scavenging, and alleviated membrane lipid peroxidation. CONCLUSIONS: This study provides comprehensive systematic information on the CeCHS gene family in tigernut, and identifies CeCHS57 as a promising candidate for molecular breeding of drought-tolerant tigernut varieties. CI - (c) 2026. The Author(s). FAU - Di, Hongli AU - Di H AD - Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, People's Republic of China. FAU - Liu, Runqing AU - Liu R AD - Heilongjiang Agricultural Engineering Technical University, Harbin, Heilongjiang Province, 150030, People's Republic of China. FAU - Zhuang, Tianning AU - Zhuang T AD - Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, People's Republic of China. FAU - Dong, Xianya AU - Dong X AD - Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, People's Republic of China. FAU - Li, Caihua AU - Li C AD - Jilin Academy of Agricultural Sciences, Changchun, Jilin Province, 130033, People's Republic of China. FAU - Mu, Zhongsheng AU - Mu Z AD - Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, People's Republic of China. AD - Jilin Academy of Agricultural Sciences, Changchun, Jilin Province, 130033, People's Republic of China. FAU - Nie, Shenglong AU - Nie S AD - Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, People's Republic of China. FAU - Cao, Di AU - Cao D AD - Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, People's Republic of China. FAU - Zhang, Shaokun AU - Zhang S AD - Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, People's Republic of China. FAU - Chen, Longji AU - Chen L AD - Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, People's Republic of China. FAU - Ding, Xin AU - Ding X AD - Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, People's Republic of China. FAU - Cai, Wentao AU - Cai W AD - Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, People's Republic of China. FAU - Liu, Conghe AU - Liu C AD - Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, People's Republic of China. FAU - Zhan, Chongzhuo AU - Zhan C AD - Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, People's Republic of China. FAU - Cheng, Yan AU - Cheng Y AD - Jilin Academy of Agricultural Sciences, Changchun, Jilin Province, 130033, People's Republic of China. FAU - Zhang, Qi AU - Zhang Q AD - Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, People's Republic of China. zqnxybynd@foxmail.com. FAU - Zheng, Wen AU - Zheng W AD - Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang Province, 163319, People's Republic of China. zhengwen6795@163.com. LA - eng GR - 2025QNTJ018/Heilongjiang Provincial Association for Science/ GR - XYB202201/Heilongjiang Bayi Agricultural University/ PT - Journal Article DEP - 20260803 PL - England TA - BMC Plant Biol JT - BMC plant biology JID - 100967807 RN - EC 2.3.- (Acyltransferases) RN - EC 2.3.1.74 (flavanone synthetase) RN - 0 (Plant Proteins) SB - IM MH - Drought Resistance MH - Phylogeny MH - *Acyltransferases/genetics/metabolism MH - *Plant Proteins/genetics/metabolism MH - Droughts MH - Stress, Physiological/genetics MH - Gene Expression Regulation, Plant MH - Arabidopsis/genetics MH - Multigene Family MH - Genome, Plant OTO - NOTNLM OT - CeCHS57 OT - Chalcone Synthase OT - Drought Stress OT - Functional verification OT - Genome-wide identification OT - Tigernut (Cyperus esculentus L.) COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: All authors approved the final manuscript and agreed to publication. Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:09 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/12 00:00 [received] PHST- 2026/07/23 00:00 [accepted] PHST- 2026/10/30 01:09 [entrez] AID - 10.1186/s12870-026-09629-w [pii] AID - 10.1186/s12870-026-09629-w [doi] PST - epublish SO - BMC Plant Biol. 2026 Aug 3;26(1):1855. doi: 10.1186/s12870-026-09629-w. PMID- 42958476 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2253 (Electronic) IS - 1471-2253 (Linking) VI - 26 IP - 1 DP - 2026 Aug 4 TI - Classical popliteal sciatic block versus Crosswise Approach to Popliteal Sciatic (CAPS) block for below-knee surgery: a randomised controlled trial. LID - 643 [pii] LID - 10.1186/s12871-026-04137-8 [doi] AB - BACKGROUND: Ultrasound-guided popliteal sciatic nerve block is frequently used for below-knee surgery. The classical approach often requires lateral decubitus or prone positioning, which may be uncomfortable for patients with lower limb injuries. The Crosswise Approach to Popliteal Sciatic (CAPS) block allows the procedure to be performed with the patient supine. This randomised trial compared the classical approach with CAPS in terms of patient comfort, block performance, and clinical outcomes. METHODS: Eighty American Society of Anesthesiologists Physical Status I-III patients undergoing unilateral below-knee surgery were randomised to receive either a classical popliteal sciatic block in the lateral position (Group A) or a CAPS block in the supine position (Group B). All procedures were ultrasound-guided with 20 mL of 0.5% ropivacaine after lignocaine skin infiltration. All patients also received a femoral nerve block. Pain scores (verbal numerical rating scale [VNRS]) during positioning and block performance, fentanyl requirements, block performance time, sensory onset time, block success, adverse effects, and patient satisfaction were assessed. RESULTS: All blocks were successful. Median highest pain scores were low in both groups (1.5 [classical] vs. 3.0 [CAPS] VNRS; p = 0.050). The proportion of patients reporting no pain (VNRS = 0) did not differ significantly between groups (30.0% [classical] vs. 15.0% [CAPS]; p = 0.180). Fentanyl supplementation (any dose) was required in 45% of Group A and 60% of Group B (p = 0.365). Block performance time was shorter with CAPS (10.5 min) than the classical approach (12.0 min; p = 0.002). Sensory onset time was similar (~ 20 min; p = 0.919). No major complications occurred. Transient sensory symptoms lasting more than 24 h occurred in two patients (5.0%) in Group A and one (2.5%) in Group B. Patient satisfaction remained high in both groups, with 75.0% of Group A and 67.5% of Group B rating satisfaction as 4 or 5 on a 5-point Likert scale (p = 0.120). CONCLUSION: Both techniques provide effective and safe anaesthesia for below-knee surgery with comparable clinical outcomes. CAPS provides a logistical advantage by allowing the procedure to be performed in the supine position, which may be of value in patients unable to tolerate lateral decubitus positioning, as well as a modest reduction in procedural time (approximately 1.5 min). Either technique may be selected based on patient factors and operator preference. TRIAL REGISTRATION: ClinicalTrials.gov (NCT07134608), registered 21 March 2025. Retrospectively registered. CI - (c) 2026. The Author(s). FAU - Teo, Rufinah AU - Teo R AD - Department of Anaesthesiology and Intensive Care, Faculty of Medicine, Hospital Canselor Tuanku Muhriz, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur, 56000, Malaysia. rufinah@hctm.ukm.edu.my. AD - Anaesthesiology Unit, Hospital Tunku Ampuan Besar Tuanku Aishah Rohani, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur, 56000, Malaysia. rufinah@hctm.ukm.edu.my. FAU - Khalid, Iskandar AU - Khalid I AD - Department of Anaesthesiology and Intensive Care, Hospital Canselor Tuanku Muhriz, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur, 56000, Malaysia. FAU - Che Kasim, Ahmad Faiz AU - Che Kasim AF AD - Department of Anaesthesiology and Intensive Care, Faculty of Medicine, Hospital Canselor Tuanku Muhriz, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur, 56000, Malaysia. FAU - Sayed Masri, Syarifah Noor Nazihah AU - Sayed Masri SNN AD - Department of Anaesthesiology and Intensive Care, Hospital Canselor Tuanku Muhriz, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur, 56000, Malaysia. FAU - Tan, Tse Siang AU - Tan TS AD - Department of Anaesthesiology and Intensive Care, Hospital Canselor Tuanku Muhriz, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur, 56000, Malaysia. FAU - Md Nor, Nadia AU - Md Nor N AD - Department of Anaesthesiology and Intensive Care, Faculty of Medicine, Hospital Canselor Tuanku Muhriz, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur, 56000, Malaysia. LA - eng SI - ClinicalTrials.gov/NCT07134608 GR - FF-2024-081/Universiti Kebangsaan Malaysia, Geran Fundamental Fakulti Perubatan (GFFP)/ PT - Comparative Study PT - Journal Article PT - Randomized Controlled Trial DEP - 20260804 PL - England TA - BMC Anesthesiol JT - BMC anesthesiology JID - 100968535 RN - 0 (Anesthetics, Local) RN - 7IO5LYA57N (Ropivacaine) SB - IM MH - Humans MH - Male MH - *Nerve Block/methods MH - *Sciatic Nerve/diagnostic imaging/drug effects MH - Ultrasonography, Interventional/methods MH - Female MH - Patient Satisfaction MH - Middle Aged MH - Adult MH - Anesthetics, Local/administration & dosage MH - Ropivacaine MH - Patient Positioning/methods MH - Pain Measurement MH - Supine Position OTO - NOTNLM OT - Anaesthesia OT - Lower Extremity OT - Pain OT - Patient Comfort OT - Sciatic Nerve OT - Supine Position OT - Ultrasonography, Interventional COIS- Declarations. Ethics approval and consent to participate: Informed consent was obtained from all subjects involved in the study. Consent for publication: Written informed consent for publication was obtained from all individuals depicted in the photographs, including the staff volunteers and the patients. The remaining figures contain anonymised ultrasound imaging with no patient-identifying information. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:09 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/14 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/30 01:09 [entrez] AID - 10.1186/s12871-026-04137-8 [pii] AID - 10.1186/s12871-026-04137-8 [doi] PST - epublish SO - BMC Anesthesiol. 2026 Aug 4;26(1):643. doi: 10.1186/s12871-026-04137-8. PMID- 42958471 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2253 (Electronic) IS - 1471-2253 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - What triggers intraoperative blood pressure/heart rate surges and postoperative hypertonia during unilateral biportal endoscopy? LID - 640 [pii] LID - 10.1186/s12871-026-04142-x [doi] AB - BACKGROUND: Unilateral biportal endoscopy (UBE) is an increasingly utilized minimally invasive technique for lumbar degenerative disease. Dural tear is a recognized complication (incidence 1.9-5.8%), typically associated with cerebrospinal fluid leakage and intracranial hypotension. However, the unique irrigation dynamics of UBE - an open two-portal system permitting high-flow continuous irrigation - may produce a distinctly different pathophysiology when an occult dural defect is present. The anesthesiology literature contains few descriptions of the acute intraoperative hemodynamic manifestations of this phenomenon. CASE PRESENTATION: A 49-year-old woman (ASA physical status I) underwent UBE for L4-L5 and L5-S1 disc herniation under total intravenous anesthesia. The procedure was complicated by intraoperative wrong-level surgery (L4-L5 misidentified as L5-S1), necessitating complete portal withdrawal, re-localization, and re-establishment of the surgical field, which prolonged the operation to 4 h 23 min with a cumulative irrigation volume of 51,000 mL. Approximately 2.5 h after incision, progressive, treatment-refractory tachycardia (70 --> 117 beats/min) and hypertension (up to 183/102 mmHg) developed; esmolol and urapidil produced minimal antihypertensive effect despite concurrent augmentation of anesthetic depth. On postoperative review, the scrub nurse recalled observing a suspected dural tear approximately 10 min before the hemodynamic escalation, which had not been communicated intraoperatively. Following extubation, the patient exhibited impaired consciousness, persistent tachypnea (40-50 breaths/min), and generalized hypertonia with decerebrate posturing. Multidisciplinary evaluation excluded epileptic seizure, metabolic encephalopathy, malignant hyperthermia, and intraoperative awareness. Suspected acute intracranial hypertension secondary to massive irrigation fluid entry into the subarachnoid space via the unrecognized dural defect was considered the most likely diagnosis. The patient was reintubated and managed in the intensive care unit with osmotic diuresis (furosemide, mannitol), corticosteroids, and sedation. Consciousness recovered the following morning, limb hypertonia resolved by postoperative day 11, and the patient was discharged on day 14 without neurological deficit. CONCLUSIONS: This case suggests that an occult dural tear during UBE with large-volume irrigation may produce a paradoxical hemodynamic profile of progressive, therapy-resistant hypertension and tachycardia - contrasting with the bradycardia and hypotension classically associated with intracranial hypotension from cerebrospinal fluid leaks. The triad of (i) progressive, treatment-refractory hypertension and tachycardia, (ii) prolonged UBE duration (> 2 h), and (iii) large-volume irrigation (> 20 L) may represent a high-risk pattern warranting immediate intraoperative communication between anesthesia and surgical teams and consideration of intracranial pressure-directed interventions. This observation is hypothesis-generating and requires validation in larger studies. CI - (c) 2026. The Author(s). FAU - Sun, Peipei AU - Sun P AD - Department of Hemodialysis Center, Hangzhou Hospital of Traditional Chinese Medicine, the Affiliated Hospital to Zhejiang Chinese Medicine University, No. 543 Tiyuchang Road, Xihu District, Hangzhou City, Zhejiang Province, China. FAU - Ma, Tingting AU - Ma T AD - Department of Anesthesiology, Tongde Hospital of Zhejiang Province, the Affiliated Hospital to Zhejiang Chinese Medicine University, No. 234, Gucui Road, Xihu District, Hangzhou, China. FAU - Ju, Jing AU - Ju J AD - Department of Operating Room, Tongde Hospital of Zhejiang Province, the Affiliated Hospital to Zhejiang Chinese Medicine University, Hangzhou, China. FAU - Pan, Min AU - Pan M AD - Department of Operating Room, Tongde Hospital of Zhejiang Province, the Affiliated Hospital to Zhejiang Chinese Medicine University, Hangzhou, China. FAU - Hu, Bingwei AU - Hu B AD - Department of Anesthesiology, Tongde Hospital of Zhejiang Province, the Affiliated Hospital to Zhejiang Chinese Medicine University, No. 234, Gucui Road, Xihu District, Hangzhou, China. whoboy555@zuaa.zju.edu.cn. LA - eng GR - 2025ZL226/Zhejiang Provincial Traditional Chinese Medicine Science and Technology Plan Project/ PT - Case Reports PT - Journal Article DEP - 20260803 PL - England TA - BMC Anesthesiol JT - BMC anesthesiology JID - 100968535 SB - IM MH - Humans MH - Female MH - Middle Aged MH - *Endoscopy/adverse effects/methods MH - *Postoperative Complications/etiology MH - *Intraoperative Complications/etiology MH - Intervertebral Disc Displacement/surgery MH - *Hypertension/etiology MH - *Tachycardia/etiology MH - Blood Pressure/physiology OTO - NOTNLM OT - Dural tear OT - Hemodynamic instability OT - Intracranial hypertension OT - Irrigation OT - Unilateral biportal endoscopy COIS- Declarations. Ethics approval and consent to participate: This retrospective case report, approved by the Institutional Review Board (IRB No. ZTD2024-172-JY; October 16, 2024). Written informed consent was obtained from the patient for publication of this case report and any accompanying images. Consent for publication: Consent for publication granted by the patient. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:08 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/06/27 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/30 01:08 [entrez] AID - 10.1186/s12871-026-04142-x [pii] AID - 10.1186/s12871-026-04142-x [doi] PST - epublish SO - BMC Anesthesiol. 2026 Aug 3;26(1):640. doi: 10.1186/s12871-026-04142-x. PMID- 42958469 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 2522-5782 (Electronic) IS - 2522-5782 (Linking) VI - 8 IP - 1 DP - 2026 Aug 1 TI - Cannabidiol selectively downregulates FAM111B expression and induces apoptosis in fibrosarcoma cells: an in vitro study. LID - 116 [pii] LID - 10.1186/s42238-026-00481-1 [doi] AB - BACKGROUND: Fibrosarcoma is a rare and aggressive soft tissue sarcoma with limited therapeutic options and poor outcomes in advanced disease. Family with sequence similarity 111 member B (FAM111B) has emerged as a pro-tumorigenic factor associated with dysregulated cell survival and proliferation. Cannabidiol (CBD), a non-psychoactive phytocannabinoid, exhibits anticancer activity in multiple malignancies, but its effects on FAM111B expression and downstream pathways in fibrosarcoma remain unclear. METHODS: The effects of CBD were evaluated in human fibrosarcoma (HT1080) cells and non-cancerous human fibroblasts (FG0). Cell metabolic activity was assessed using an MTT assay. Gene and protein expression were determined by real-time quantitative PCR and Western blotting, respectively. Apoptosis and cell-cycle distribution were assessed by flow cytometry using Annexin V-FITC/propidium iodide (PI) and PI staining alone, respectively. Statistical analyses were performed using an unpaired Student's t-test or a two-way analysis of variance (ANOVA) followed by Sidak's post hoc multiple comparisons test, as appropriate. RESULTS: CBD reduced metabolic activity in both HT1080 and FG0 cells in a dose-dependent manner, with comparable IC(5)(0) values (28.95 +/- 3.15 microM and 30.75 +/- 9.12 microM, respectively), indicating limited selectivity based on metabolic activity alone. CBD significantly reduced FAM111B mRNA expression by approximately 3-fold in HT1080 cells and 2-fold in FG0 cells; however, a significant reduction in FAM111B protein expression was observed only in HT1080 cells, CBD treatment was associated with marked downregulation of BAG3 and Cyclin B1 proteins, increased apoptosis (Annexin V-positive cells: 3.55 +/- 0.78% vs. 13.20 +/- 4.56%), and altered cell-cycle progression characterized by increased Sub-G1 (3.44 +/- 1.10% vs. 18.37 +/- 0.83%) and G0/G1 (28.30 +/- 1.57% vs. 39.17 +/- 0.85%) populations, with corresponding reductions in the S- and G2/M-phase populations. These effects were markedly attenuated in non-cancerous fibroblasts. CONCLUSIONS: CBD exerts context-dependent anticancer effects in HT1080 fibrosarcoma cells by selectively suppressing FAM111B protein expression, promoting apoptosis, and disrupting cell-cycle progression. These findings identify FAM111B- and BAG3-associated pathways as potential therapeutic vulnerabilities and support further investigation of CBD as a modulator of stress-adaptive and proliferative signaling in preclinical fibrosarcoma models. CI - (c) 2026. The Author(s). FAU - Naicker, Danielle AU - Naicker D AD - Hair and Skin Research Laboratory, Division of Dermatology, Department of Medicine, University of Cape Town, Cape Town, South Africa. FAU - Sunda, Falone AU - Sunda F AD - Hair and Skin Research Laboratory, Division of Dermatology, Department of Medicine, University of Cape Town, Cape Town, South Africa. FAU - Rhoda, Cenza AU - Rhoda C AD - Hair and Skin Research Laboratory, Division of Dermatology, Department of Medicine, University of Cape Town, Cape Town, South Africa. FAU - Mutavhatsindi, Hygon AU - Mutavhatsindi H AD - Biomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg, South Africa. AD - Department of Medicine, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa. FAU - Khumalo, Nonhlanhla AU - Khumalo N AD - Hair and Skin Research Laboratory, Division of Dermatology, Department of Medicine, University of Cape Town, Cape Town, South Africa. FAU - Arowolo, Afolake AU - Arowolo A AD - Hair and Skin Research Laboratory, Division of Dermatology, Department of Medicine, University of Cape Town, Cape Town, South Africa. afolake.arowolo@uct.ac.za. AD - Biomedical Research and Innovation Platform, South African Medical Research Council, Tygerberg, South Africa. afolake.arowolo@uct.ac.za. LA - eng GR - TTK170413227114/South African National Research Foundation (NRF) Thuthuka Funding Instrument (NRF Rating Track)/ GR - HDID8682/MB2022/South African Medical Research Council/ PT - Journal Article DEP - 20260801 PL - England TA - J Cannabis Res JT - Journal of cannabis research JID - 101752723 OTO - NOTNLM OT - Apoptosis OT - BAG3 OT - Cannabidiol OT - Cell-cycle OT - FAM111B OT - Fibrosarcoma OT - Flow cytometry COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:08 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/02 00:00 [received] PHST- 2026/07/26 00:00 [accepted] PHST- 2026/10/30 01:08 [entrez] AID - 10.1186/s42238-026-00481-1 [pii] AID - 10.1186/s42238-026-00481-1 [doi] PST - epublish SO - J Cannabis Res. 2026 Aug 1;8(1):116. doi: 10.1186/s42238-026-00481-1. PMID- 42958461 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2072-1315 (Electronic) IS - 1606-0997 (Linking) VI - 45 IP - 1 DP - 2026 Aug 2 TI - Association of medical visit frequency with cardiovascular events and mortality in patients with dyslipidaemia: a cohort study using a Japanese claims database. LID - 241 [pii] LID - 10.1186/s41043-026-01408-5 [doi] AB - BACKGROUND: Dyslipidaemia is a major risk factor for cardiovascular and cerebrovascular diseases; however, the optimal frequency of medical visits remains unclear. OBJECTIVE: To examine whether medical visit frequency is associated with the incidence of cardiac events, cerebrovascular events, and mortality among patients initiating lipid-lowering therapy. METHODS: We conducted a retrospective cohort study using data from the Shizuoka Kokuho Database between April 1, 2012, and September 30, 2021. Patients initiating lipid-lowering therapy were classified into high-frequency (>/= 22 visits in 2 years) and low-frequency (8-12 visits in 2 years) groups. After 1:3 propensity score matching and exclusion of patients with events within 2 years, 1,435 patients in the low-frequency group and 4,412 in the high-frequency group were analysed. RESULTS: Over a mean follow-up of 5.3 years, composite outcomes occurred in 5.6% of both groups (hazard ratio [HR] 1.08; 95% confidence interval [CI] 0.84-1.38; p = 0.571). No significant differences were observed for cardiac events (HR 1.13; 95% CI 0.83-1.54), cerebrovascular events (HR 1.13; 95% CI 0.67-1.90), or mortality (HR 0.94; 95% CI 0.60-1.48). CONCLUSION: Less frequent visits were not associated with increased risks of cardiovascular events or mortality. Extending visit intervals may therefore be a reasonable option for selected stable patients receiving lipid-lowering therapy for primary prevention. CI - (c) 2026. The Author(s). FAU - Matsunaga, Taku AU - Matsunaga T AD - Graduate School of Public Health, Shizuoka Graduate University of Public Health, 4-27-2 Kitaando, Aoi-ku, Shizuoka, 420-0881, Japan. AD - Department of General Medicine, Fujieda Municipal General Hospital, Shizuoka, Japan. FAU - Nakatani, Eiji AU - Nakatani E AUID- ORCID: 0000-0002-4876-446X AD - Graduate School of Public Health, Shizuoka Graduate University of Public Health, 4-27-2 Kitaando, Aoi-ku, Shizuoka, 420-0881, Japan. nakatani.eiji.int@gmail.com. AD - Laboratory of Biostatistics, NHO Nagoya Medical Center, 4-1-1 Sannomaru, Naka-ku, Nagoya, 460-0001, Japan. nakatani.eiji.int@gmail.com. FAU - Sato, Yasuto AU - Sato Y AD - Graduate School of Public Health, Shizuoka Graduate University of Public Health, 4-27-2 Kitaando, Aoi-ku, Shizuoka, 420-0881, Japan. AD - Center for Health Informatics Policy, National Institute of Public Health, Saitama, Japan. FAU - Kaneda, Hideaki AU - Kaneda H AD - Okinaka Memorial Institute for Medical Research, Tokyo, Japan. FAU - Ohno, Hiromitsu AU - Ohno H AD - Allied Medical K.K, Tokyo, Japan. FAU - Sugawara, Akira AU - Sugawara A AD - Graduate School of Public Health, Shizuoka Graduate University of Public Health, 4-27-2 Kitaando, Aoi-ku, Shizuoka, 420-0881, Japan. asugawara@s-sph.ac.jp. LA - eng GR - Nothing/Shizuoka Prefecture/ PT - Journal Article DEP - 20260802 PL - Bangladesh TA - J Health Popul Nutr JT - Journal of health, population, and nutrition JID - 100959228 RN - 0 (Hypolipidemic Agents) RN - Japanese people SB - IM MH - Humans MH - *Dyslipidemias/mortality/complications/drug therapy MH - Female MH - Japan/epidemiology MH - *Cardiovascular Diseases/epidemiology/mortality/etiology MH - Male MH - Retrospective Studies MH - Aged MH - Middle Aged MH - Databases, Factual MH - Hypolipidemic Agents/therapeutic use MH - Incidence MH - Cerebrovascular Disorders/epidemiology MH - Risk Factors MH - *Office Visits/statistics & numerical data MH - East Asian People OTO - NOTNLM OT - Cardiovascular diseases OT - Dyslipidaemias OT - Mortality OT - Patient follow-up OT - Primary health care OT - Stroke COIS- Declarations. Ethics approval: The study was approved by the ethics committee of the Graduate School of Public Health, Shizuoka Graduate University of Public Health, Japan (SGUPH_2021_001_074 - 2). Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:08 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/04 00:00 [received] PHST- 2026/07/07 00:00 [accepted] PHST- 2026/10/30 01:08 [entrez] AID - 10.1186/s41043-026-01408-5 [pii] AID - 10.1186/s41043-026-01408-5 [doi] PST - epublish SO - J Health Popul Nutr. 2026 Aug 2;45(1):241. doi: 10.1186/s41043-026-01408-5. PMID- 42958458 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1601-5223 (Electronic) IS - 0018-0661 (Linking) VI - 163 IP - 1 DP - 2026 Jul 31 TI - Esketamine induces ferroptosis and suppresses immune evasion in lung adenocarcinoma cells via the hsa_circ_0022392/miR-548b-3p/MAP7 axis. LID - 122 [pii] LID - 10.1186/s41065-026-00713-7 [doi] AB - BACKGROUND: Although Esketamine, an N-methyl-D-aspartate (NMDA) receptor antagonist, is widely used for anesthesia and analgesia and has shown anticancer activity, its effects on lung adenocarcinoma remain unclear. Circular RNAs have been implicated in the development and progression of lung adenocarcinoma. Therefore, this study aims to elucidate whether esketamine can influence lung adenocarcinoma progression by interfering with the circular RNA hsa_circ_0022392. METHODS: Expression levels of hsa_circ_0022392, miR-548b-3p, and microtubule-associated protein 7 (MAP7), as well as transfection efficiency, were assessed by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot analysis. Cell viability was assessed using the Cell Counting Kit-8 assay. 5-ethynyl-2'-deoxyuridine (EdU) assay was employed to examine cell proliferation. Transwell assays were employed for analyzing cell migration and invasion capabilities. Intracellular Fe(2)(+) levels, malondialdehyde (MDA), glutathione (GSH), and reactive oxygen species (ROS) were measured using commercial kits. Lactate dehydrogenase (LDH) release assays were adopted to evaluate CD8(+) T-cell cytotoxicity. Cytokine levels were measured via ELISA. Bioinformatics analysis combined with RNA immunoprecipitation and dual-luciferase reporter assays were employed to confirm the target relationship between miR-548b-3p and hsa_circ_0022392 or MAP7. RESULTS: Esketamine significantly inhibited proliferation, migration, and invasion (all P < 0.05), induced ferroptosis (P < 0.05), and suppressed immune evasion (P < 0.05). Notably, hsa_circ_0022392 expression was upregulated in lung adenocarcinoma tissues and cell lines (P < 0.01), and esketamine downregulated its levels (P < 0.05). Overexpressed hsa_circ_0022392 notably reversed esketamine's inhibitory effect on lung adenocarcinoma cell progression (P < 0.05). Further studies revealed that hsa_circ_0022392 targeted and bound to miR-548b-3p, thereby upregulating MAP7 expression (P < 0.05). Interfering with this signaling axis effectively weakened the inhibitory effect of esketamine on lung adenocarcinoma progression (P < 0.05). CONCLUSION: In summary, esketamine promotes ferroptosis in lung adenocarcinoma cells through regulating the hsa_circ_0022392/miR-548b-3p/MAP7 axis and inhibits immune evasion, providing novel molecular targets and therapeutic strategies for the diagnosis and treatment of lung adenocarcinoma. CI - (c) 2026. The Author(s). FAU - Chai, Fei AU - Chai F AD - Department of Anesthesiology, Affiliated Hospital of Hebei University, No. 212 Yuhua East Road, Lianchi District, Baoding City, Hebei Province, 071000, China. FAU - Zhang, Yang AU - Zhang Y AD - Department of Anesthesiology, Affiliated Hospital of Hebei University, No. 212 Yuhua East Road, Lianchi District, Baoding City, Hebei Province, 071000, China. FAU - Chen, Xing AU - Chen X AD - Department of Emergency Medicine, Dingzhou People's Hospital, Baoding City, Hebei Province, 073000, China. FAU - Geng, XiaoYun AU - Geng X AD - Department of General Surgery, Baoding Fourth Central Hospital, Baoding City, Hebei Province, 072750, China. FAU - Kang, XinJuan AU - Kang X AD - Department of General Surgery, Baoding Fourth Central Hospital, Baoding City, Hebei Province, 072750, China. FAU - Wan, Hua AU - Wan H AD - Department of General Surgery, Baoding Fourth Central Hospital, Baoding City, Hebei Province, 072750, China. FAU - Du, XiaoBing AU - Du X AD - Department of Anesthesiology, Affiliated Hospital of Hebei University, No. 212 Yuhua East Road, Lianchi District, Baoding City, Hebei Province, 071000, China. du_xiaobing9036@outlook.com. LA - eng GR - No. 2441ZF107/Baoding Science and Technology Plan Project/ PT - Journal Article DEP - 20260731 PL - England TA - Hereditas JT - Hereditas JID - 0374654 RN - 0 (MicroRNAs) RN - 0 (RNA, Circular) RN - 0 (Microtubule-Associated Proteins) SB - IM MH - Humans MH - *MicroRNAs/genetics MH - *Adenocarcinoma of Lung/genetics/immunology/drug therapy MH - *Ferroptosis/drug effects MH - Cell Line, Tumor MH - *Lung Neoplasms/genetics/immunology/drug therapy MH - *RNA, Circular/genetics MH - *Microtubule-Associated Proteins/genetics/metabolism MH - Gene Expression Regulation, Neoplastic/drug effects MH - Cell Proliferation/drug effects OTO - NOTNLM OT - Esketamine OT - Ferroptosis OT - Immune evasion OT - Lung adenocarcinoma OT - hsa_circ_0022392/miR-548b-3p/MAP7 signaling axis COIS- Declarations. Ethics approval and consent to participate: The present study was approved by the Ethics Committee of Affiliated Hospital of Hebei University (HDFYLL-KY-2024-214) and written informed consent was provided by all patients prior to the study start. All procedures were performed in accordance with the ethical standards of the Institutional Review Board and The Declaration of Helsinki, and its later amendments or comparable ethical standards. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:08 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/14 00:00 [received] PHST- 2026/07/13 00:00 [accepted] PHST- 2026/10/30 01:08 [entrez] AID - 10.1186/s41065-026-00713-7 [pii] AID - 10.1186/s41065-026-00713-7 [doi] PST - epublish SO - Hereditas. 2026 Jul 31;163(1):122. doi: 10.1186/s41065-026-00713-7. PMID- 42958454 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 1758-2946 (Print) IS - 1758-2946 (Linking) VI - 18 IP - 1 DP - 2026 Jul 31 TI - Transformer-based molecular fragment prediction using SMILES and DeepSMILES representations in a fragment-based drug discovery pipeline. LID - 148 [pii] LID - 10.1186/s13321-026-01255-w [doi] AB - Fragment-based drug discovery (FBDD) uses small molecular fragments as starting points for drug development, and machine-learning models that operate on molecular string representations are increasingly applied to fragment-related tasks. SMILES is the dominant such representation, but its paired ring closures and balanced parentheses introduce syntactic complexity that can affect model behavior. We present a controlled study of how molecular string representation influences transformer-based fragment recovery, using an FBDD-motivated label pipeline: reference fragments are derived from known drugs via RECAP fragmentation and docking-based ranking, and the model is scored on recovering them. We introduce DeepBERTa, a ChemBERTa-derived transformer pretrained on DeepSMILES, and evaluate it against a matched SMILES baseline under identical architecture, data splits, and optimization (approximately 34,000 drug-fragment pairs). DeepSMILES produces syntactically valid predictions more often than SMILES (54.2% vs. 43.5%) and a higher full test-set mean Tanimoto similarity (0.36 vs. 0.29). A per-sample selection between the two representations raises mean Tanimoto to 0.43, and a deployable variant that selects on model confidence rather than the reference recovers most of this gain. Among molecules where one representation strictly wins, DeepSMILES wins more often than SMILES (27.8% vs. 16.3% of test molecules). These results show that string representation meaningfully influences transformer fragment recovery and that representation-aware selection outperforms either representation alone. CI - (c) 2026. The Author(s). FAU - Kothari, Aayush AU - Kothari A AD - Department of Computer Science & Engineering, Aspiring Scholars Directed Research Program, Fremont, CA, 94539, USA. FAU - Gupta, Amish AU - Gupta A AD - Department of Computer Science & Engineering, Aspiring Scholars Directed Research Program, Fremont, CA, 94539, USA. FAU - Shah, Nisarg AU - Shah N AD - Department of Chemistry, Biochemistry, and Physical Science, Aspiring Scholars Directed Research Program, Fremont, CA, 94539, USA. FAU - Reed, Thomas AU - Reed T AD - Department of Chemistry, Biochemistry, and Physical Science, Aspiring Scholars Directed Research Program, Fremont, CA, 94539, USA. FAU - Nattuva, Anvita AU - Nattuva A AD - Department of Chemistry, Biochemistry, and Physical Science, Aspiring Scholars Directed Research Program, Fremont, CA, 94539, USA. FAU - Nazirudeen, Rahima AU - Nazirudeen R AD - Department of Chemistry, Biochemistry, and Physical Science, Aspiring Scholars Directed Research Program, Fremont, CA, 94539, USA. FAU - Brah, Harman AU - Brah H AD - Department of Chemistry, Biochemistry, and Physical Science, Aspiring Scholars Directed Research Program, Fremont, CA, 94539, USA. FAU - Akl, Marx AU - Akl M AD - Department of Computer Science & Engineering, Aspiring Scholars Directed Research Program, Fremont, CA, 94539, USA. marx.akl@asdrp.org. LA - eng PT - Journal Article DEP - 20260731 PL - England TA - J Cheminform JT - Journal of cheminformatics JID - 101516718 OTO - NOTNLM OT - ChemBERTa OT - DeepSMILES OT - Drug design OT - Fragment-based drug discovery OT - Masked language modeling OT - SMILES OT - Tanimoto similarity OT - Transformers COIS- Declarations. Ethics approval and consent to participate: Not applicable. This study used publicly available molecular datasets and computationally generated data. No human participants, clinical data, or animal experiments were involved. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:07 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/05 00:00 [received] PHST- 2026/06/25 00:00 [accepted] PHST- 2026/10/30 01:07 [entrez] AID - 10.1186/s13321-026-01255-w [pii] AID - 10.1186/s13321-026-01255-w [doi] PST - epublish SO - J Cheminform. 2026 Jul 31;18(1):148. doi: 10.1186/s13321-026-01255-w. PMID- 42958447 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2042-6410 (Electronic) IS - 2042-6410 (Linking) VI - 17 IP - 1 DP - 2026 Aug 2 TI - Sex differences in murine bone matrix maturation modulate osteoblast-endothelial interactions. LID - 173 [pii] LID - 10.1186/s13293-026-00960-3 [doi] AB - BACKGROUND: Bone formation during skeletal growth and repair is divergently modulated by osteoblast-derived vascular endothelial growth factor (VEGF), which contributes to the sexual dimorphism of the bone vasculature. While the extracellular matrix (ECM) provides structural and instructive cues to developing vasculature, whether the osteoblast-derived matrix contributes to this dimorphism remains unclear. METHODS: Primary osteoblasts from the long bones of neonatal female and male C57BL/6J mice were cultured under basal or osteogenic conditions for compositional ECM analysis by Raman spectroscopy. Primary murine bone marrow-derived endothelial cells (BMECs) were seeded onto established osteoblast layers and maintained in heterotypic cocultures to assess contact-mediated effects of osteoblast ECM on BMEC survival and expansion. Osteoblast-derived conditioned media (CM) were used to evaluate soluble-factor contributions, with VEGF-A concentration quantified by ELISA. RESULTS: Raman spectroscopy of monocultured osteoblasts revealed sexually dimorphic ECM signatures independent of cellular growth profiles. Female matrices were enriched with type I collagen-associated proline and hydroxyproline and octacalcium phosphate, consistent with a matrix-dominant signature. Male matrices exhibited lower levels of collagen-associated components and instead adopted a more mineral-mature profile, reflected by CAP accumulation and an elevated mineral/matrix ratio. In heterotypic cocultures, BMEC numbers were 1.39-fold higher with male than female osteoblasts. CM treatment of BMECs did not recapitulate these effects despite higher VEGF-A release from male osteoblasts. CONCLUSIONS: Sex differences in osteoblast-derived ECM are linked to divergent, contact-dependent modulation of BMEC behaviour. These findings indicate that intrinsic sex differences in osteoblast matrix maturation may contribute to sex-specific regulation of the skeletal vascular niche. Defining how osteoblast-derived ECM regulates skeletal vascularisation may reveal targets for selectively modulating pathological skeletal angiogenesis in women and men. CI - (c) 2026. The Author(s). FAU - Sharma, Aikta AU - Sharma A AUID- ORCID: 0000-0002-5449-358X AD - Department of Mechanical Engineering, University College London, London, WC1E 7JE, UK. Aikta.sharma@ucl.ac.uk. AD - School of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK. Aikta.sharma@ucl.ac.uk. FAU - Emery, Roger J H AU - Emery RJH AD - The Hamlyn Centre for Robotic Surgery, Imperial College London, London, SW7 2AZ, UK. FAU - Pitsillides, Andrew A AU - Pitsillides AA AD - Department of Comparative Biomedical Sciences, Royal Veterinary College, London, NW1 0TU, UK. FAU - Clarkin, Claire E AU - Clarkin CE AD - School of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK. C.E.Clarkin@soton.ac.uk. LA - eng PT - Journal Article DEP - 20260802 PL - England TA - Biol Sex Differ JT - Biology of sex differences JID - 101548963 RN - 0 (Vascular Endothelial Growth Factor A) SB - IM MH - Animals MH - Female MH - *Osteoblasts/physiology/cytology/metabolism MH - Male MH - *Sex Characteristics MH - *Bone Matrix/physiology/metabolism MH - Mice, Inbred C57BL MH - *Endothelial Cells/physiology/cytology MH - Extracellular Matrix/metabolism MH - Mice MH - Cells, Cultured MH - Coculture Techniques MH - Vascular Endothelial Growth Factor A/metabolism MH - *Cell Communication MH - Spectrum Analysis, Raman OAB - Bone is a sexually dimorphic organ, with women and men differing in bone size, strength and risk of fracture. The skeletal vasculature is essential for bone growth and repair, with bone forming osteoblast cells influencing blood vessel development through the skeletal extracellular matrix (ECM). Although the interactions between osteoblast and vascular cells are crucial for lifelong skeletal health, whether sex differences in bone structure between women and men arise from differences in osteoblast activity or sex differences in blood vessel growth remains unknown. Here, we show that female and male mouse osteoblasts deposit compositionally distinct ECMs that differentially influence vascular endothelial cell behaviour. Female osteoblasts produce a collagen-rich matrix with low mineral content. In contrast, male osteoblasts produce matrices containing less collagen and more mineral, while releasing elevated levels of the blood vessel-promoting factor, VEGF-A, compared with female osteoblasts. When placed directly onto these osteoblast layers, vascular cell growth was greater in cocultures with male than female osteoblasts and could not be reproduced by exposure to osteoblast-derived soluble factors alone. These findings identify a contact-dependent relationship through which sex differences in osteoblast ECM composition influence vascular cell behaviour in bone. Understanding how osteoblast-vascular interactions differ by sex may explain the variability in bone health, healing capacity and disease risk between women and men. Further, our approach may inform the discovery of new therapeutic targets that support bone growth and repair while targeting abnormal blood vessel growth in a sex-specific manner. OABL- eng OTO - NOTNLM OT - Collagen OT - Endothelial cell OT - Extracellular matrix OT - Osteoblast OT - Raman spectroscopy OT - Sex dimorphism OT - VEGF COIS- Declarations. Ethics approval and consent to participate: All experimental procedures were performed in accordance with the UK Animals (Scientific Procedures) Act of 1986 and regulations set by the UK Home Office and local institutional guidelines. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:07 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/27 00:00 [received] PHST- 2026/07/23 00:00 [accepted] PHST- 2026/10/30 01:07 [entrez] AID - 10.1186/s13293-026-00960-3 [pii] AID - 10.1186/s13293-026-00960-3 [doi] PST - epublish SO - Biol Sex Differ. 2026 Aug 2;17(1):173. doi: 10.1186/s13293-026-00960-3. PMID- 42958443 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 2162-3619 (Print) IS - 2162-3619 (Linking) VI - 15 IP - 1 DP - 2026 Oct 28 TI - HER2 in solid tumors: microenvironment features and multifaceted advances from mechanisms to clinical translation. LID - 114 [pii] LID - 10.1186/s40164-026-00850-9 [doi] AB - Human epidermal growth factor receptor 2 (HER2) is a well-established oncogenic driver in multiple solid tumors, most prominently in breast and gastric cancers. Beyond gene amplification-driven signaling through canonical PI3K/AKT and RAS/MAPK pathways, emerging evidence reveals that HER2 functions within a highly dynamic and context-dependent tumor ecosystem. Interactions between HER2-positive tumor cells and immune and stromal compartments actively shape the tumor microenvironment, contributing to immune evasion, therapeutic resistance and disease progression. A central challenge in the clinical management of HER2-altered cancers is pronounced spatial and temporal heterogeneity, which undermines both diagnostic accuracy and the durability of targeted therapies. In response, advances in multi-omics profiling, liquid biopsy technologies and artificial intelligence-assisted analytics are redefining HER2 assessment, shifting it from static tissue-based classification towards dynamic, minimally invasive monitoring. In parallel, the therapeutic landscape has evolved from single-agent HER2 blockade to rational combination strategies. In particular, antibody-drug conjugates have emerged as a transformative modality, extending the therapeutic reach of HER2 targeting beyond traditional indications and offering new opportunities to overcome heterogeneity and drug resistance. This review synthesizes current insights into the molecular and microenvironmental biology of HER2-altered solid tumors and discusses how these advances are reshaping next-generation precision oncology. CI - (c) 2026. The Author(s). FAU - Zhang, Tianqi AU - Zhang T AD - Department of Pathology, Fudan University Shanghai Cancer Center, 270 Dong'an Road, Xuhui District, Shanghai, 200032, China. AD - Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. AD - Institute of Pathology, Fudan University, Shanghai, 200032, China. FAU - Tan, Cong AU - Tan C AD - Department of Pathology, Fudan University Shanghai Cancer Center, 270 Dong'an Road, Xuhui District, Shanghai, 200032, China. AD - Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. AD - Institute of Pathology, Fudan University, Shanghai, 200032, China. FAU - Sun, Hui AU - Sun H AD - Department of Pathology, Fudan University Shanghai Cancer Center, 270 Dong'an Road, Xuhui District, Shanghai, 200032, China. AD - Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. AD - Institute of Pathology, Fudan University, Shanghai, 200032, China. FAU - Gu, Wenchao AU - Gu W AD - Department of Artificial Intelligence Medicine, Graduate School of Medicine, Chiba University, Chiba, Japan. AD - Department of Diagnostic and Interventional Radiology, University of Tsukuba, Tsukuba, Ibaraki, Japan. FAU - Wang, Wenfeng AU - Wang W AD - Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. wangwenfeng1011@163.com. AD - Shanghai Urological Cancer Institute, Cancer Institute, Fudan University Shanghai Cancer Center, Fudan University, 270 Dong'an Road, Xuhui District, Shanghai, 200032, China. wangwenfeng1011@163.com. FAU - Xu, Midie AU - Xu M AD - Department of Pathology, Fudan University Shanghai Cancer Center, 270 Dong'an Road, Xuhui District, Shanghai, 200032, China. xumd27202003@sina.com. AD - Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. xumd27202003@sina.com. AD - Institute of Pathology, Fudan University, Shanghai, 200032, China. xumd27202003@sina.com. FAU - Sheng, Weiqi AU - Sheng W AD - Department of Pathology, Fudan University Shanghai Cancer Center, 270 Dong'an Road, Xuhui District, Shanghai, 200032, China. shengweiqi2006@163.com. AD - Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. shengweiqi2006@163.com. AD - Institute of Pathology, Fudan University, Shanghai, 200032, China. shengweiqi2006@163.com. LA - eng GR - 82473011/National Natural Science Foundation of China/ GR - 82573096/National Natural Science Foundation of China/ PT - Journal Article PT - Review DEP - 20261028 PL - England TA - Exp Hematol Oncol JT - Experimental hematology & oncology JID - 101590676 OTO - NOTNLM OT - Artificial intelligence OT - Combination therapy OT - HER2 OT - Heterogeneity OT - Tumor microenvironment COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: The authors confirm their consent to publish the manuscript. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:07 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/26 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/30 01:07 [entrez] AID - 10.1186/s40164-026-00850-9 [pii] AID - 10.1186/s40164-026-00850-9 [doi] PST - epublish SO - Exp Hematol Oncol. 2026 Oct 28;15(1):114. doi: 10.1186/s40164-026-00850-9. PMID- 42958435 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1756-994X (Electronic) IS - 1756-994X (Linking) VI - 18 IP - 1 DP - 2026 Oct 17 TI - Multikingdom and functional gut microbiota markers for cholangiocarcinoma. LID - 168 [pii] LID - 10.1186/s13073-026-01757-0 [doi] AB - BACKGROUND: While the association between gut microbiota and cholangiocarcinoma (CCA) has been explored, existing research has been limited to bacterial components. The alterations in non-bacterial members (fungi, archaea, and viruses) and their functional characteristics in CCA remain unclear. This study aimed to comprehensively characterize the multi-kingdom gut microbiota and functional profiles in CCA patients and develop a non-invasive diagnostic model. METHODS: We performed metagenomic sequencing on 700 fecal samples collected from three distinct regions in China, comprising 289 CCA patients, 312 healthy controls, and 99 hepatocellular carcinoma (HCC) patients. Multi-kingdom microbial composition and functional profiles were analyzed. We integrated multiple machine learning algorithms to generate 117 distinct modeling schemes for constructing and screening the optimal CCA diagnostic model. The model was validated across three independent cohorts and tested for specificity against HCC. We also benchmarked the model against established diagnostic approaches by reviewing 15 CCA clinical practice guidelines/consensus statements and their cited evidence and by evaluating model detection in CA19-9-positive and CA19-9-negative patients. RESULTS: Extensive alterations were identified in CCA patients across multi-kingdom and functional layers, including 198 archaeal, 154 bacterial, 78 fungal, and 120 viral species, as well as 278 genes, 99 pathways, and 151 carbohydrate-active enzymes (CAZymes). A panel of 21 multi-kingdom and functional markers demonstrated superior diagnostic accuracy with an average AUC of 91% across three validation cohorts, outperforming any single-kingdom model. The model exhibited high specificity in distinguishing CCA from HCC, achieving an AUC of 99.74% with a false positive rate of 11.11%. Benchmarking supported a complementary role as a noninvasive screening aid, the model showed pooled sensitivity and specificity of 96.2%, and 78.5%, respectively, and detected 97.6% of CA19-9-negative CCA cases. CONCLUSIONS: This study represents the first comprehensive characterization of multi-kingdom gut microbiome and functional markers in cholangiocarcinoma. Gut multi-kingdom and functional microbial signatures represent a promising noninvasive tool that may aid the screening and early identification of cholangiocarcinoma in high-risk populations, complementing existing diagnostic approaches. CI - (c) 2026. The Author(s). FAU - Rao, Benchen AU - Rao B AD - Department of Infectious Diseases, State Key Laboratory of Antiviral Drugs, Pingyuan Laboratory, The First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. AD - Gene Hospital of Henan Province/Precision Medicine Center, the First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. FAU - Liu, Shanshuo AU - Liu S AD - Department of Infectious Diseases, State Key Laboratory of Antiviral Drugs, Pingyuan Laboratory, The First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. AD - Gene Hospital of Henan Province/Precision Medicine Center, the First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. FAU - Cui, Guangying AU - Cui G AD - Department of Infectious Diseases, State Key Laboratory of Antiviral Drugs, Pingyuan Laboratory, The First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. AD - Gene Hospital of Henan Province/Precision Medicine Center, the First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. FAU - Jiang, Jianwen AU - Jiang J AD - Health Management Center, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China. FAU - Li, Na AU - Li N AD - Department of Infectious Diseases, State Key Laboratory of Antiviral Drugs, Pingyuan Laboratory, The First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. AD - Gene Hospital of Henan Province/Precision Medicine Center, the First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. FAU - Hu, Ji AU - Hu J AD - Department of Infectious Diseases, State Key Laboratory of Antiviral Drugs, Pingyuan Laboratory, The First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. FAU - Sun, Ranran AU - Sun R AD - Department of Infectious Diseases, State Key Laboratory of Antiviral Drugs, Pingyuan Laboratory, The First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. AD - Gene Hospital of Henan Province/Precision Medicine Center, the First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. FAU - Li, Ang AU - Li A AD - Department of Infectious Diseases, State Key Laboratory of Antiviral Drugs, Pingyuan Laboratory, The First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. AD - Gene Hospital of Henan Province/Precision Medicine Center, the First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. FAU - Zhang, Ruiqing AU - Zhang R AD - Department of Hepatobiliary and Echinococcosis Surgery, Digestive and Vascular Surgery Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830054, China. FAU - Lu, Haifeng AU - Lu H AD - State Key Laboratory for Diagnosis and Treatment of Infectious Disease, National Clinical Research Center for Infectious Diseases, Department of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003, China. FAU - Wen, Peihao AU - Wen P AD - Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450000, China. FAU - Xue, Jianfeng AU - Xue J AD - Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450000, China. FAU - Aji, Tuerganaili AU - Aji T AD - Department of Hepatobiliary and Echinococcosis Surgery, Digestive and Vascular Surgery Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830054, China. FAU - Wang, Haiyu AU - Wang H AD - Department of Infectious Diseases, State Key Laboratory of Antiviral Drugs, Pingyuan Laboratory, The First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. AD - Gene Hospital of Henan Province/Precision Medicine Center, the First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. FAU - Chen, Xiaolong AU - Chen X AD - Department of Infectious Diseases, State Key Laboratory of Antiviral Drugs, Pingyuan Laboratory, The First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. AD - Gene Hospital of Henan Province/Precision Medicine Center, the First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. FAU - Zheng, Shusen AU - Zheng S AD - Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China. shusenzheng@zju.edu.cn. FAU - Yu, Zujiang AU - Yu Z AD - Department of Infectious Diseases, State Key Laboratory of Antiviral Drugs, Pingyuan Laboratory, The First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. AD - Gene Hospital of Henan Province/Precision Medicine Center, the First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. FAU - Ren, Zhigang AU - Ren Z AUID- ORCID: 0000-0003-0798-3444 AD - Department of Infectious Diseases, State Key Laboratory of Antiviral Drugs, Pingyuan Laboratory, The First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. fccrenzg@zzu.edu.cn. AD - Gene Hospital of Henan Province/Precision Medicine Center, the First Affiliated Hospital of Zhengzhou University, 1#, Jianshe East Road, Zhengzhou, 450052, China. fccrenzg@zzu.edu.cn. LA - eng GR - 82570691/National Natural Science Foundation of China/ GR - 81672422/National Natural Science Foundation of China/ GR - 82470654/National Natural Science Foundation of China/ GR - 2022D01C219/Xinjiang Uygur Autonomous Region Natural Science Foundation/ GR - JNL-2025007B/Research Project of Jinan Microecological Biomedicine Shandong Laboratory/ GR - 2025ZD01906305/Prevention and Control of Emerging and Major Infectious Diseases-National Science and Technology Major Project/ GR - 24HASTIT063/University Science and Technology Innovation Talent Support Plan of Henan Province/ GR - HNCMS202423/Henan Province "Three 100" Program, Special Project for Clinical Medical Scientist Cultivation/ PT - Journal Article DEP - 20261017 PL - England TA - Genome Med JT - Genome medicine JID - 101475844 RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - *Cholangiocarcinoma/microbiology/diagnosis MH - *Gastrointestinal Microbiome MH - *Bile Duct Neoplasms/microbiology/diagnosis MH - Female MH - Metagenomics/methods MH - Male MH - Middle Aged MH - Feces/microbiology MH - Liver Neoplasms/microbiology/diagnosis MH - Biomarkers, Tumor MH - Metagenome MH - Carcinoma, Hepatocellular/microbiology MH - Aged OTO - NOTNLM OT - Cholangiocarcinoma OT - Diagnostic model OT - Gut microbiota OT - Liver cancer OT - Metagenome OT - Multikingdom microbiota COIS- Declarations. Ethics approval and consent to participate: This study has been conducted in accordance with the ethical standards of the Declaration of Helsinki and. This study was approved by the Ethics Committee of First Affiliated Hospital, School of Medicine, Zhejiang University (2017 - 611, 2016-220 and 2014-334), the First Affiliated Hospital of Zhengzhou University (Xie-2019-039 and 2017-XY-002), the First Affiliated Hospital of Xinjiang Medical University (20170427-11). Written informed consent was obtained from all participants prior to enrollment. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:07 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2025/12/11 00:00 [received] PHST- 2026/08/31 00:00 [accepted] PHST- 2026/10/30 01:07 [entrez] AID - 10.1186/s13073-026-01757-0 [pii] AID - 10.1186/s13073-026-01757-0 [doi] PST - epublish SO - Genome Med. 2026 Oct 17;18(1):168. doi: 10.1186/s13073-026-01757-0. PMID- 42958432 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2050-7283 (Electronic) IS - 2050-7283 (Linking) VI - 14 IP - 1 DP - 2026 Aug 1 TI - School-weariness is associated with enhanced emotional stroop interference and selective autonomic differences in junior middle school students. LID - 1563 [pii] LID - 10.1186/s40359-026-05265-z [doi] AB - BACKGROUND: School-weariness (SW) is a pattern of persistent negative affect toward learning and school, with cognitive devaluation of academic activities. SW is associated with reduced academic motivation, poorer academic functioning, and internalizing/somatic problems, but its cognitive-affective processes and autonomic correlates remain unclear. This study examined whether SW is associated with enhanced emotional Stroop interference and selective physiological differences in junior middle school students. METHODS: This exploratory cross-sectional matched case-control study recruited 47 junior middle school students from three schools in Hangzhou, China. Participants completed questionnaires and an emotional Stroop task with electrocardiographic recording during baseline, task, and recovery. Behavioral outcomes were analyzed using linear mixed-effects models or binomial generalized linear mixed-effects models, and electrocardiography (ECG) / heart rate variability (HRV) outcomes were analyzed using linear mixed-effects models. Age, gender, and school source were included as covariates where appropriate. RESULTS: Compared with controls, the SW group showed higher scores in all symptom scales, slower reaction times, and greater emotional Stroop interference. Accuracy effects were not significant. For the ECG results, the SW group showed higher heart rate across baseline, task, and recovery. The HRV measures showed no robust group-related pattern. CONCLUSIONS: SW in junior middle school students was characterized by greater internalizing/somatic burden, slower responding, and greater interference from school-related negative words, together with limited exploratory autonomic differences. CI - (c) 2026. The Author(s). FAU - Yu, Shuyang AU - Yu S AD - Center for Cognition and Brain Disorders, Affiliated Hospital of Hangzhou Normal University, Hangzhou, China. AD - Zhejiang Key Laboratory for Research in Assessment for Cognitive Impairments, Hangzhou, China. FAU - Song, Qianwen AU - Song Q AD - Center for Cognition and Brain Disorders, Affiliated Hospital of Hangzhou Normal University, Hangzhou, China. AD - Zhejiang Key Laboratory for Research in Assessment for Cognitive Impairments, Hangzhou, China. FAU - Lu, Huixin AU - Lu H AD - Center for Cognition and Brain Disorders, Affiliated Hospital of Hangzhou Normal University, Hangzhou, China. AD - Zhejiang Key Laboratory for Research in Assessment for Cognitive Impairments, Hangzhou, China. FAU - Luo, Hong AU - Luo H AD - Affiliated Mental Health Center & Hangzhou Seventh People's Hospital, Zhejiang University School of Medicine, Hangzhou, China. luohong@vip.126.com. FAU - Hu, Zhiguo AU - Hu Z AD - Center for Cognition and Brain Disorders, Affiliated Hospital of Hangzhou Normal University, Hangzhou, China. huzg@hznu.edu.cn. AD - Zhejiang Key Laboratory for Research in Assessment for Cognitive Impairments, Hangzhou, China. huzg@hznu.edu.cn. AD - Department of Neurology, Affiliated Hospital of Hangzhou Normal University, Hangzhou, China. huzg@hznu.edu.cn. LA - eng GR - No. XS25B015/the National Center for Mental Health, China; the China Education Development Foundation; and the Center for Student Services and Development, Ministry of Education, P.R. China./ PT - Journal Article DEP - 20260801 PL - England TA - BMC Psychol JT - BMC psychology JID - 101627676 SB - IM MH - Humans MH - Female MH - Male MH - Adolescent MH - Stroop Test MH - Case-Control Studies MH - *Students/psychology MH - Child MH - Cross-Sectional Studies MH - *Emotions/physiology MH - *Heart Rate/physiology MH - Schools MH - China MH - Electrocardiography MH - *Autonomic Nervous System/physiology MH - Reaction Time/physiology OTO - NOTNLM OT - Attentional interference OT - Emotional Stroop task OT - Heart rate variability OT - Junior middle school students OT - School-weariness COIS- Declarations. Ethics approval and consent to participate: The study protocol was approved by the Ethics Committee of the Affiliated Hospital of Hangzhou Normal University [approval number: 2025(E2)-KS-140]. All procedures involving human participants were conducted in accordance with the Declaration of Helsinki and applicable institutional and national ethical guidelines and regulations. Written informed consent was obtained from all participants and their legal guardians prior to participation. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:06 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/04/29 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/30 01:06 [entrez] AID - 10.1186/s40359-026-05265-z [pii] AID - 10.1186/s40359-026-05265-z [doi] PST - epublish SO - BMC Psychol. 2026 Aug 1;14(1):1563. doi: 10.1186/s40359-026-05265-z. PMID- 42958425 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1757-2215 (Electronic) IS - 1757-2215 (Linking) VI - 19 IP - 1 DP - 2026 Aug 1 TI - Targeting monocyte dysregulation in ovarian cancer: insights from single-cell genomics and Mendelian randomization. LID - 328 [pii] LID - 10.1186/s13048-026-02217-4 [doi] AB - BACKGROUND: Ovarian cancer (OC) lethality is frequently associated with an immunosuppressive tumor immune microenvironment (TIME). While monocytes appear to function as notable regulators within the TIME, their causal involvement in OC pathogenesis remains poorly defined. METHOD: To investigate the causal involvement of monocytes in OC, we integrated single-cell RNA sequencing datasets from the GEO and TISCH databases. Comparative cell proportion analysis was conducted using Fisher's exact test. Cell-cell communication networks were mapped with CellChat to characterize tumor-specific interactions via ligand-receptor pairs (e.g., MIF, MDK). Causal associations were identified through Summary-data-based Mendelian randomization (MR) and two-sample MR, integrating eQTLGen statistics (n = 31,684) with OC GWAS data (1,588 cases). Instrumental variables were prioritized based on F-statistics > 10 and LD clumping. Bayesian colocalization and the HEIDI test (P > 0.05) were employed to rule out linkage disequilibrium. Finally, functional roles of candidate genes were validated via qRT-PCR, Western blot, and in vitro polarization assays. RESULT: Quantitative analysis revealed a significant enrichment of monocytes in the OC group (24.58%) compared to healthy controls (2.44%, P < 0.001), which was independently validated by immunofluorescence staining. CellChat identified tumor-specific signaling axes, including MIF-(CD74 + CXCR4) and MDK-NCL, that were predominantly active in the OC microenvironment but nearly quiescent in healthy tissues. We identified five monocyte-related genes-SGK1, PRKCA, TBC1D4, VPS8, and PPFIBP2-that demonstrated consistent causal associations with OC risk and passed the HEIDI test (P > 0.05), ruling out linkage disequilibrium. Functional enrichment suggested these genes modulate PI3K-Akt and MAPK signaling. In vitro assays confirmed that SGK1 is specifically upregulated in M2-polarized macrophages. Furthermore, pharmacological inhibition of SGK1 using si113 significantly reversed the immunosuppressive M2 phenotype and impaired tumor cell invasion, highlighting its potential as a precision therapeutic target. CONCLUSION: This study integrates single-cell transcriptomics and MR to identify five monocyte-related genes causally linked to OC risk. Among them, SGK1 may play a substantial role in promoting immunosuppressive macrophage polarization and may represent a promising therapeutic target to remodel the TIME and enhance anti-tumor immunity in OC. CI - (c) 2026. The Author(s). FAU - Ou, Li AU - Ou L AD - Department of Obstetrics and Gynaecology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 210009, China. AD - Nanjing Key Laboratory of Intelligent Obstetrics and Gynecology Medicine, Nanjing, 210009, China. FAU - Lin, Hao AU - Lin H AD - Department of Obstetrics and Gynaecology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 210009, China. AD - Nanjing Key Laboratory of Intelligent Obstetrics and Gynecology Medicine, Nanjing, 210009, China. FAU - Ding, Bo AU - Ding B AD - Department of Obstetrics and Gynaecology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 210009, China. bodingno1@163.com. AD - Nanjing Key Laboratory of Intelligent Obstetrics and Gynecology Medicine, Nanjing, 210009, China. bodingno1@163.com. FAU - Shen, Yang AU - Shen Y AD - Department of Obstetrics and Gynaecology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, 210009, China. shenyang@seu.edu.cn. AD - Nanjing Key Laboratory of Intelligent Obstetrics and Gynecology Medicine, Nanjing, 210009, China. shenyang@seu.edu.cn. LA - eng GR - No. 82372126/the National Natural Science Foundation of China/ GR - No. 82372126/the National Natural Science Foundation of China/ GR - No. 82372126/the National Natural Science Foundation of China/ GR - No. 82372126/the National Natural Science Foundation of China/ PT - Journal Article DEP - 20260801 PL - England TA - J Ovarian Res JT - Journal of ovarian research JID - 101474849 SB - IM MH - Female MH - Humans MH - *Ovarian Neoplasms/genetics/pathology/metabolism MH - *Monocytes/metabolism/immunology MH - Single-Cell Analysis/methods MH - *Genomics/methods MH - Mendelian Randomization Analysis/methods MH - Tumor Microenvironment MH - Genome-Wide Association Study MH - Single-Cell Gene Expression Analysis OTO - NOTNLM OT - Bayesian colocalization OT - Mendelian randomization OT - Monocytes OT - Ovarian cancer OT - Single-cell RNA sequencing OT - Tumor immune microenvironment COIS- Declarations. Ethics approval and consent to participate: This study was approved by the Ethics Committee of Zhongda Hospital, Southeast University (BR2015021) and conducted in accordance with the Declaration of Helsinki. Informed consent was waived due to the retrospective nature of the study. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:06 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2025/06/21 00:00 [received] PHST- 2026/07/21 00:00 [accepted] PHST- 2026/10/30 01:06 [entrez] AID - 10.1186/s13048-026-02217-4 [pii] AID - 10.1186/s13048-026-02217-4 [doi] PST - epublish SO - J Ovarian Res. 2026 Aug 1;19(1):328. doi: 10.1186/s13048-026-02217-4. PMID- 42958422 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1752-1947 (Electronic) IS - 1752-1947 (Linking) VI - 20 IP - 1 DP - 2026 Aug 3 TI - Tazemetostat combined PD-1 inhibitor achieving prolonged survival in primary proximal epithelioid sarcoma: a case report and review of the literature. LID - 666 [pii] LID - 10.1186/s13256-026-06312-4 [doi] AB - BACKGROUND: Epithelioid sarcoma (ES) is a rare mesenchymal tumor categorized into proximal (axial) and classical (distal) types. In general, proximal ES come alone with limited treatment options and poor prognosis. Herein, we present the case of a proximal ES treated with a tazemetostat-containing regimen and achieved a favorable response in the third-line setting. CASE DESCRIPTION: A 44-year-old Han woman was noticed a left pelvic floor nodule by computed tomography (CT) scan, and histologically test result confirmed her diagnosis as epithelioid sarcoma (proximal type). Cell staining indicates a negative expression of INI1 but positive expression of PD-L1. Ifosfamide and adriamycin (IA) were introduced as first-line treatment because of multidisciplinary treatment (MDT) of soft-tissue tumor considered tumor invasion is extensive, not eligible to complete surgical resection. Unfortunately, IA was discontinued due to patient intolerance (severe myelosuppression). Anlotinib was used as second-line treatment after rapid disease progression and also discontinued due to vaginal hemorrhage. After the pathology diagnosis, the combination therapy of tislelizumab and tazemetostat was introduced. A significant tumor shrinkage was noticed after 21 months of follow-up, which achieved and continue to maintain a partial response (PR) with further tumor reduction. CONCLUSIONS: This case first reported a favorable response of primary proximal epithelioid sarcoma treated with EZH2 inhibitor tazemetostat and PD-1 inhibitor tislelizumab, suggesting a potential and well-tolerated treatment regimen for this rare disease. CI - (c) 2026. The Author(s). FAU - Li, Wei AU - Li W AD - Department of Medical Oncology, Zhongshan Hospital, Fudan University, No. 180, Fenglin Road, Xuhui District, Shanghai, China. FAU - Zhang, Chenlu AU - Zhang C AD - Department of Medical Oncology, Zhongshan Hospital, Fudan University, No. 180, Fenglin Road, Xuhui District, Shanghai, China. FAU - You, Yang AU - You Y AD - Department of Medical Oncology, Zhongshan Hospital, Fudan University, No. 180, Fenglin Road, Xuhui District, Shanghai, China. FAU - Guo, Xi AU - Guo X AD - Department of Medical Oncology, Zhongshan Hospital, Fudan University, No. 180, Fenglin Road, Xuhui District, Shanghai, China. FAU - Luo, Rongkui AU - Luo R AD - Department of Pathology, Zhongshan Hospital, Fudan University, Shanghai, China. FAU - Zhou, Yuhong AU - Zhou Y AD - Department of Medical Oncology, Zhongshan Hospital, Fudan University, No. 180, Fenglin Road, Xuhui District, Shanghai, China. zhou.yuhong@zs-hospital.sh.cn. LA - eng PT - Case Reports PT - Journal Article PT - Review DEP - 20260803 PL - England TA - J Med Case Rep JT - Journal of medical case reports JID - 101293382 RN - 0 (Pyridones) RN - 0 (Biphenyl Compounds) RN - Q40W93WPE1 (tazemetostat) RN - 0 (Morpholines) RN - 0KVO411B3N (tislelizumab) RN - 0 (Benzamides) RN - 80168379AG (Doxorubicin) RN - 0 (Immune Checkpoint Inhibitors) RN - 0 (Indoles) RN - 0 (Thiazoles) RN - 0 (anlotinib) RN - UM20QQM95Y (Ifosfamide) RN - 0 (Quinolines) RN - 0 (Antibodies, Monoclonal, Humanized) SB - IM MH - Humans MH - Female MH - *Sarcoma/drug therapy/pathology/diagnostic imaging MH - Adult MH - *Pyridones/therapeutic use/administration & dosage MH - *Biphenyl Compounds/therapeutic use/administration & dosage MH - *Antineoplastic Combined Chemotherapy Protocols/therapeutic use MH - *Morpholines/therapeutic use/administration & dosage MH - *Benzamides/therapeutic use MH - Treatment Outcome MH - Doxorubicin MH - *Immune Checkpoint Inhibitors/therapeutic use MH - Indoles MH - *Thiazoles/therapeutic use/administration & dosage MH - Ifosfamide MH - Quinolines MH - Antibodies, Monoclonal, Humanized OTO - NOTNLM OT - Case report OT - EZH2 inhibitor OT - Epithelioid sarcoma OT - INI-1 OT - Pelvic COIS- Declarations. Ethics approval and consent to participate: This retrospective chart review study involving human participants complied with institutional and national research committee ethical standards, as well as the 1964 Declaration of Helsinki and its later amendments. This study was approved by the Ethics Committee of Zhongshan Hospital, Fudan University, Shanghai, China. This is an observational study. (Ethical approval ID: B2020-338). Informed consent was obtained from the patients. Consent for publication: Written informed consent was obtained from the patient for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal. Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:06 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2025/01/24 00:00 [received] PHST- 2026/06/15 00:00 [accepted] PHST- 2026/10/30 01:06 [entrez] AID - 10.1186/s13256-026-06312-4 [pii] AID - 10.1186/s13256-026-06312-4 [doi] PST - epublish SO - J Med Case Rep. 2026 Aug 3;20(1):666. doi: 10.1186/s13256-026-06312-4. PMID- 42958414 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1756-9966 (Electronic) IS - 0392-9078 (Linking) VI - 45 IP - 1 DP - 2026 Aug 3 TI - PROTAC technology for sensitizing melanoma to immune checkpoint therapy. LID - 226 [pii] LID - 10.1186/s13046-026-03799-z [doi] AB - Melanoma remains one of the most aggressive malignancies, with limited response to immune checkpoint inhibitors (ICIs) due to primary or acquired resistance. Proteolysis-targeting chimeras (PROTACs) have emerged as a novel therapeutic modality that induces selective degradation of target proteins via the ubiquitin-proteasome system, offering distinct advantages over conventional inhibitors. This review summarizes the design principles and molecular mechanisms of PROTACs, and highlights their emerging role in sensitizing melanoma to immunotherapy. Specifically, PROTACs can restore tumor antigen presentation, reprogram the immunosuppressive tumor microenvironment by targeting regulatory T cells, myeloid-derived suppressor cells, and tumor-associated macrophages, and suppress key oncogenic signaling pathways. We also discuss combination strategies with PD-1/PD-L1 and CTLA-4 blockade, as well as current challenges including poor cell permeability, off-target effects, and acquired resistance. Finally, future perspectives on rational design, tissue-specific delivery systems, and clinical translation are addressed. CI - (c) 2026. The Author(s). FAU - Zhou, Qiujun AU - Zhou Q AD - Department of Dermatology, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310003, China. FAU - Zhang, Xiaowei AU - Zhang X AD - Physical Examination Center, The Fourth Affiliated Hospital of China Medical University, Shenyang, 110032, Liaoning, China. FAU - Zhao, Chenlu AU - Zhao C AD - Department of First Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310000, China. FAU - Chen, Difei AU - Chen D AD - Department of Clinical Medicine, He University, Shenyang, Liaoning, 110163, China. FAU - Chu, Chen AU - Chu C AD - Department of General Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, 110032, China. chuchen20260630@163.com. FAU - Jin, Xiaoliang AU - Jin X AUID- ORCID: 0000-0001-8768-3534 AD - The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, 310003, China. jin_xiaoliang@foxmail.com. LA - eng GR - 2025RCZXZK58/the Research Project of Zhejiang Chinese Medical University/ PT - Journal Article PT - Review DEP - 20260803 PL - England TA - J Exp Clin Cancer Res JT - Journal of experimental & clinical cancer research : CR JID - 8308647 RN - 0 (Proteolysis Targeting Chimera) RN - 0 (Immune Checkpoint Inhibitors) SB - IM MH - Humans MH - Proteolysis Targeting Chimera MH - *Immune Checkpoint Inhibitors/therapeutic use/pharmacology MH - *Melanoma/drug therapy/immunology/pathology MH - *Immunotherapy/methods MH - Animals MH - Tumor Microenvironment OTO - NOTNLM OT - Immune checkpoint blockade OT - Immunotherapy OT - Melanoma OT - PROTACs OT - Targeted protein degradation COIS- Declarations. Ethics approval and consent to participate: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:05 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/05/20 00:00 [received] PHST- 2026/07/30 00:00 [accepted] PHST- 2026/10/30 01:05 [entrez] AID - 10.1186/s13046-026-03799-z [pii] AID - 10.1186/s13046-026-03799-z [doi] PST - epublish SO - J Exp Clin Cancer Res. 2026 Aug 3;45(1):226. doi: 10.1186/s13046-026-03799-z. PMID- 42958412 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1742-4755 (Electronic) IS - 1742-4755 (Linking) VI - 23 IP - 1 DP - 2026 Jul 30 TI - Respectful maternity care and its determinants in public health facilities of thar desert district, India: a mixed methods approach. LID - 197 [pii] LID - 10.1186/s12978-026-02427-5 [doi] AB - BACKGROUND: With the increasing proportion of institutional deliveries, improving the quality of maternity services is essential to ensure a positive childbirth experience. Respectful Maternity Care (RMC) has emerged as a key component in achieving this goal. This study aims to estimate the prevalence and determinants of RMC and to explore the perceptions of perinatal women and their caregivers in public health facilities of Jodhpur. METHODS: A concurrent parallel mixed-methods study was conducted among 438 perinatal women attending health facilities in Jodhpur district. Using a multistage random sampling approach, four rural and nine urban facilities were selected. Data were collected through semi-structured interviews with perinatal women and their caregivers, supplemented by in-depth interviews with those reporting lack of RMC. Quantitative data were analyzed using regression models to identify determinants of RMC, while qualitative data were thematically analyzed and triangulated with quantitative findings. RESULTS: Of the 438 enrolled participants, 407 completed the study. The overall prevalence of respectful maternity care (RMC) was low at 4.42%, with the domain of right to information, informed consent, and choice of preferences scoring only 12.28%. Other domains were substantially higher: protection from physical harm or ill-treatment (93.36%), never being detained at a health facility (95.08%), confidential care (92.12%), dignified care (77.64%), equal care without discrimination (86.24%), and never being denied care (78.62%). Higher parity (three or more births) emerged as a significant determinant of RMC. Mode of delivery with intervention such as episiotomy or LSCS (B=-0.222, p = 0.049) and the absence of a labour companion (B=-0.969, p = 0.006) were significantly associated with poor perception of RMC. Thematic analysis of in-depth interviews with women and caregivers highlighted experiences of physical and verbal abuse, neglect during care, and contextual factors underlying such mistreatment. CONCLUSION: The study highlights that several aspects of respectful maternity care are already being practiced in public health facilities, demonstrating the potential for further improvement. Nevertheless, the overall prevalence remains low, primarily due to the limited practice of informed choice and consent. Women who underwent delivery interventions such as episiotomy or caesarean section, as well as those without a labour companion, were more likely to report poor perception of Respectful Maternity Care. The qualitative findings underscore persistent challenges of abuse and neglect, emphasizing the urgent need for system-level interventions, including sensitization of healthcare providers, strengthening accountability mechanisms, and enhancing supportive infrastructure, to ensure a positive and dignified childbirth experience. CI - (c) 2026. The Author(s). FAU - Gowda, Bindiya C AU - Gowda BC AD - Department of Community Medicine and Family Medicine, All India Institute of Medical Sciences, Marudhar Industrial Area, MIA phase 2, Basni, Jodhpur, Rajasthan, 342005, India. AD - Department of Community Medicine, Siddaganga Medical College and Research Institute (SMCRI), Tumkur, Karnataka, India. FAU - Rustagi, Neeti AU - Rustagi N AUID- ORCID: 0000-0003-3051-5411 AD - Department of Community Medicine and Family Medicine, All India Institute of Medical Sciences, Marudhar Industrial Area, MIA phase 2, Basni, Jodhpur, Rajasthan, 342005, India. drneetigoswami@gmail.com. FAU - Mittal, Ankit AU - Mittal A AD - Department of Community Medicine, Dr. S S Tantia Medical College Hospital and Research Centre, Sri Ganganagar, Rajasthan, India. FAU - Thirunavukkarasu, Prasanna AU - Thirunavukkarasu P AD - Department of Community Medicine and Family Medicine, All India Institute of Medical Sciences, Marudhar Industrial Area, MIA phase 2, Basni, Jodhpur, Rajasthan, 342005, India. FAU - Srinivasan, Srikanth AU - Srinivasan S AD - Department of Community Medicine and Family Medicine, All India Institute of Medical Sciences, Marudhar Industrial Area, MIA phase 2, Basni, Jodhpur, Rajasthan, 342005, India. FAU - Sachin, J AU - Sachin J AD - Junior Resident, Department of Pharmacology, All India Institute of Medical Sciences, Jodhpur, Rajasthan, India. AD - Department of Pharmacology, Siddaganga Medical College and Research Institute (SMCRI), Tumkur, Karnataka, India. FAU - Chattu, Vijay Kumar AU - Chattu VK AD - Faculty of Medicine, University of Toronto, Toronto, Canada. LA - eng GR - MD21JUN-0178/Indian Council of Medical Research/ PT - Journal Article DEP - 20260730 PL - England TA - Reprod Health JT - Reproductive health JID - 101224380 SB - IM MH - Humans MH - Female MH - *Maternal Health Services/standards MH - Pregnancy MH - Adult MH - India MH - *Respect MH - *Quality of Health Care MH - Young Adult MH - Delivery, Obstetric MH - Health Facilities OTO - NOTNLM OT - Disrespect and abuse OT - Intranatal care OT - Maternal health services OT - Quality of care OT - RMC determinants OT - Respectful maternity care COIS- Declarations. Ethical approval: Ethical approval was obtained from the Institutional Ethical Committee, AIIMS Jodhpur, vide their Ethical Clearance Certificate reference no. AIIMS/IEC/2021/3347, dated 12/03/2021. Informed consent was obtained from all participants and their confidentiality was maintained. Competing interests: The authors declare no competing interests EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:05 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2025/05/26 00:00 [received] PHST- 2026/07/26 00:00 [accepted] PHST- 2026/10/30 01:05 [entrez] AID - 10.1186/s12978-026-02427-5 [pii] AID - 10.1186/s12978-026-02427-5 [doi] PST - epublish SO - Reprod Health. 2026 Jul 30;23(1):197. doi: 10.1186/s12978-026-02427-5. PMID- 42958410 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1749-8090 (Electronic) IS - 1749-8090 (Linking) VI - 21 IP - 1 DP - 2026 Aug 1 TI - B‑lines on lung ultrasound: a non‑invasive marker for short‑term outcomes after coronary artery bypass grafting. LID - 863 [pii] LID - 10.1186/s13019-026-04573-0 [doi] AB - BACKGROUND: Postoperative pulmonary complications increase morbidity and mortality after coronary artery bypass grafting (CABG). Lung ultrasound detection of B-lines is an established method to quantify pulmonary congestion, and while B-lines have been studied as predictive markers in heart failure and general critical care settings, their role in predicting outcomes specifically for post-CABG patients remains inadequately defined. This study aimed to evaluate the association between B-lines detected on postoperative lung ultrasound and 30-day mortality and hospital length of stay in patients undergoing isolated CABG. METHODS: This prospective cohort study was conducted in a single-center intensive care unit between 2023 and 2024. A total of 148 adult patients scheduled for elective CABG were enrolled. Lung ultrasound was performed on postoperative days 1 (POD1) and 3 (POD3) using a standardized 8-zone protocol to quantify B-lines. The primary outcomes were all-cause 30-day mortality, assessed via hospital records and telephone follow-up, and total hospital length of stay. Univariate and multivariate logistic and linear regression analyses were used to identify predictors. RESULTS: The mean patient age was 60.15 +/- 9.8 years, and 57% were male. Within 30 days, 22 patients (15%) died. In the multivariate logistic regression, the number of B-lines on POD1 was an independent predictor of 30-day mortality (Adjusted Odds Ratio: 1.090, 95% CI: 1.018-1.167, p = 0.013), indicating a 9% increase in the odds of death for each additional B-line. In contrast, the B-line count on POD3 was not significantly associated with mortality. B-line counts on both days were also not associated with hospital length of stay. Instead, higher body mass index (beta = 0.22, p = 0.022) and a history of smoking (beta = 2.20, p = 0.020) were independently associated with longer hospitalization. CONCLUSIONS: Early postoperative pulmonary congestion, as quantified by B-lines on POD1 lung ultrasound, is an independent predictor of 30-day mortality after CABG. Routine bedside lung ultrasound on the first day after surgery can serve as a non-invasive tool to stratify high-risk patients, potentially allowing for earlier intervention to optimize fluid status and improve outcomes. CI - (c) 2026. The Author(s). FAU - Afrakoti, Nima Mohamadi AU - Afrakoti NM AUID- ORCID: 0009-0005-6979-3111 AD - Department of Anesthesilogy, Dr Ali Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran. FAU - Khani, Mahan AU - Khani M AD - Tehran University of Medical Sciences, Tehran, Iran. FAU - Hemmati, Sina AU - Hemmati S AD - Tehran University of Medical Sciences, Tehran, Iran. FAU - Ebrahiminasab, Ali AU - Ebrahiminasab A AD - Tehran University of Medical Sciences, Tehran, Iran. FAU - Yekta, Reza Atef AU - Yekta RA AD - Department of Anesthesilogy, Dr Ali Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran. FAU - Rabi, Samaneh Hajy Molla AU - Rabi SHM AD - Department of Neurology, Dr Rajaee Hospital, Alborz University of Medical Sciences, Alborz, Iran. FAU - Pagard, Elham AU - Pagard E AD - Shariati Hospital, Cardiovascular Surgical ICU, Tehran University of Medical Sciences, Tehran, Iran. FAU - Marashi, Mojtaba AU - Marashi M AUID- ORCID: 0000-0003-3757-6305 AD - Department of Anesthesilogy, Dr Ali Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran. marashi@tums.ac.ir. LA - eng PT - Journal Article DEP - 20260801 PL - England TA - J Cardiothorac Surg JT - Journal of cardiothoracic surgery JID - 101265113 SB - IM MH - Humans MH - Male MH - *Coronary Artery Bypass/adverse effects MH - Female MH - Prospective Studies MH - Middle Aged MH - Ultrasonography/methods MH - *Postoperative Complications/diagnostic imaging/mortality MH - *Lung/diagnostic imaging MH - Length of Stay MH - Aged MH - Treatment Outcome OTO - NOTNLM OT - B-lines OT - Cardiac anesthesia OT - Coronary artery bypass grafting OT - Critical care monitoring OT - Lung ultrasound OT - Mortality OT - Postoperative complications OT - Prognosis OT - Pulmonary congestion COIS- Declarations. Ethics approval and consent to participate: The study protocol was reviewed and approved by the Ethics Committee of Tehran University of Medical Sciences (Approval ID: IR.TUMS.SHARIATI.REC.1402.148). Written informed consent was obtained from all participants or their legal representatives prior to enrollment. All procedures were conducted in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:05 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2025/09/03 00:00 [received] PHST- 2026/07/11 00:00 [accepted] PHST- 2026/10/30 01:05 [entrez] AID - 10.1186/s13019-026-04573-0 [pii] AID - 10.1186/s13019-026-04573-0 [doi] PST - epublish SO - J Cardiothorac Surg. 2026 Aug 1;21(1):863. doi: 10.1186/s13019-026-04573-0. PMID- 42958405 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1479-5876 (Electronic) IS - 1479-5876 (Linking) VI - 24 IP - 1 DP - 2026 Aug 3 TI - Deep N-glycoproteomic profiling of FFPE tissues enables precise diagnosis of primary liver cancer from metastatic liver cancer. LID - 1374 [pii] LID - 10.1186/s12967-026-08747-1 [doi] AB - BACKGROUND: Reliable tumor origin assessment from routinely archived specimens remains challenging, particularly for diagnostically ambiguous liver malignancies. Although formalin-fixed, paraffin-embedded (FFPE) tissues are widely available, deep and quantitative site-specific N-glycoproteomics of these specimens is limited by formaldehyde-induced crosslinking and incomplete peptide recovery. METHODS: We developed GlyFFPE, a quantitative platform for site-specific N-glycosylation profiling from FFPE archival tissues, and applied it to discovery and independent verification datasets from colorectal cancer liver metastasis (CRLM) and liver hepatocellular carcinoma (LIHC) patients. We then integrated glyco-signatures with clinicopathological variables using an ensemble machine-learning classifier and evaluated performance in a temporal validation cohort. Model robustness and incremental value were assessed using a pathology-only comparator, repeated nested cross-validation, feature-selection stability analysis, and a same-institution temporal cohort. RESULTS: GlyFFPE enabled deep site-specific N-glycoproteome profiling from FFPE tissues and revealed metastasis-associated glyco-signatures characterized by elevated levels of high-mannose glycans on cell-adhesion molecules, prominently CEACAM1 and CEACAM5. The two-glycopeptide panel from CEACAM high-mannose signature showed modest discrimination between CRLM and LIHC in an independent TMT-PRM verification cohort (AUC = 0.73), indicating the need of expanding the targeted panel. The model integrating glyco-signatures with clinicopathological variables substantially improved diagnostic performance, achieving glycoproteomic-assisted discrimination in a same-institution temporal cohort. Repeated nested cross-validation provided a more conservative performance estimate with a mean AUC of 0.92 and a mean AUPRC of 0.96. Feature-selection analysis identified repeatedly selected molecular features, although moderate feature-set variability remained because of the limited cohort size. In addition to classification, GlyFFPE revealed metastasis-associated glycosylation remodeling, including increased high-mannose occupancy on alpha-1-acid glycoprotein 1 (ORM1) and coordinated alterations in extracellular matrix-associated glycoproteins. CONCLUSION: GlyFFPE establishes an FFPE-compatible workflow for site-specific N-glycosylation profiling and identifies candidate molecular signatures that complement routine pathological assessment for tumor origin evaluation in challenging liver malignancies. Given the modest cohort size and the same-institution temporal evaluation, multicenter, blinded external validation are required before clinical implementation. CI - (c) 2026. The Author(s). FAU - Li, Hanjie AU - Li H AD - Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, 214122, China. AD - Key Laboratory of Biopharmaceutical Preparation and Delivery, Chinese Academy of Sciences, Beijing, 100190, China. FAU - Bai, Ruizhen AU - Bai R AD - Department of Pathology, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214122, China. FAU - Huang, Chenyu AU - Huang C AD - Key Laboratory of Biopharmaceutical Preparation and Delivery, Chinese Academy of Sciences, Beijing, 100190, China. AD - Key Laboratory of Advanced Process Control for Light Industry (Ministry of Education), The School of IOT Engineering, Jiangnan University, Wuxi, Jiangsu, China. FAU - Wang, Xiuyuan AU - Wang X AD - Key Laboratory of Biopharmaceutical Preparation and Delivery, Chinese Academy of Sciences, Beijing, 100190, China. FAU - Yu, Zeyang AU - Yu Z AD - Key Laboratory of Biopharmaceutical Preparation and Delivery, Chinese Academy of Sciences, Beijing, 100190, China. FAU - Zhou, Biao AU - Zhou B AD - Key Laboratory of Advanced Process Control for Light Industry (Ministry of Education), The School of IOT Engineering, Jiangnan University, Wuxi, Jiangsu, China. FAU - Yuan, Ye AU - Yuan Y AD - Key Laboratory of Biopharmaceutical Preparation and Delivery, Chinese Academy of Sciences, Beijing, 100190, China. FAU - Liu, Quan AU - Liu Q AD - Department of Medical Oncology, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214122, China. quanliu@jiangnan.edu.cn. FAU - Yang, Ganglong AU - Yang G AD - Key Laboratory of Biopharmaceutical Preparation and Delivery, Chinese Academy of Sciences, Beijing, 100190, China. glyang@ipe.ac.cn. FAU - Gao, Xiao-Dong AU - Gao XD AUID- ORCID: 0000-0002-8575-5095 AD - Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, 214122, China. xdgao@ipe.ac.cn. AD - Key Laboratory of Biopharmaceutical Preparation and Delivery, Chinese Academy of Sciences, Beijing, 100190, China. xdgao@ipe.ac.cn. LA - eng GR - 32271342/National Natural Science Foundation of China/ PT - Journal Article DEP - 20260803 PL - England TA - J Transl Med JT - Journal of translational medicine JID - 101190741 RN - 0 (Glycoproteins) RN - 1HG84L3525 (Formaldehyde) SB - IM MH - Humans MH - *Liver Neoplasms/diagnosis/pathology/metabolism/secondary MH - *Paraffin Embedding MH - *Proteomics/methods MH - *Tissue Fixation MH - *Glycoproteins/metabolism MH - *Formaldehyde/chemistry MH - Neoplasm Metastasis MH - Glycosylation MH - Reproducibility of Results COIS- Declarations. Ethics approval and consent to participate: All human tissue samples used in this study were obtained from patients with informed consent. The study protocol was reviewed and approved by the Institutional Review Board of Affiliated Hospital of Jiangnan University (Approval No. LS2021-048). The collection and use of tissue samples were conducted in accordance with the Declaration of Helsinki and all relevant national regulations. No interventional procedures were performed on patients solely for research purposes. Disclaimer: None. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:05 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/05/12 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/30 01:05 [entrez] AID - 10.1186/s12967-026-08747-1 [pii] AID - 10.1186/s12967-026-08747-1 [doi] PST - epublish SO - J Transl Med. 2026 Aug 3;24(1):1374. doi: 10.1186/s12967-026-08747-1. PMID- 42958401 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1479-5876 (Electronic) IS - 1479-5876 (Linking) VI - 24 IP - 1 DP - 2026 Jul 31 TI - Target-area dosimetry-guided photobiomodulation improves pathology and cognitive function in APPswe/PS1dE9 mice. LID - 1368 [pii] LID - 10.1186/s12967-026-08734-6 [doi] AB - BACKGROUND: Photobiomodulation (PBM) has shown potential in Alzheimer's disease (AD) models, but substantial heterogeneity in irradiation parameters and the lack of intracerebral dose quantification have limited reproducibility and mechanistic interpretation. In transcranial PBM, surface power density does not reliably reflect energy delivery to brain targets due to wavelength-dependent tissue attenuation. METHODS: We developed a target-area dosimetry- guided PBM framework by integrating Monte Carlo-based light transport modeling with inverse calibration for selected representative wavelength-target conditions. Based on these simulations, inverse calibration was performed for selected representative wavelength-target combinations, with 810 nm irradiation optimized for PFC-directed PBM and 1060 nm, 40 Hz pulsed irradiation optimized for HIPP-directed PBM. APPswe/PS1dE9 mice received region-oriented and comparative PBM treatments, followed by behavioral, pathological, and electrophysiological assessments. RESULTS: Monte Carlo simulations revealed marked wavelength-dependent differences in intracerebral light distribution under identical surface irradiation. Inverse calibration supported the selection of region-specific irradiation parameters and highlighted the need to consider target-area light exposure rather than surface power alone. Among the tested irradiation conditions, PBM improved spatial learning and memory and modulated amyloid burden, neuroinflammatory responses, synaptic markers, and neural network activity. Among these treatments, combined hippocampal irradiation with 810 nm and 1060 nm light produced the most consistent overall responses, while 810 nm PFC irradiation also showed beneficial effects across several outcome measures. CONCLUSIONS: These findings support target-area dosimetry as an important physical consideration for the design and interpretation of region-oriented PBM protocols in AD models. By integrating intracerebral light propagation modeling with biological evaluation, this study provides a methodological framework for improving PBM parameter selection and guiding future optimization of transcranial PBM interventions. CI - (c) 2026. The Author(s). FAU - Cao, Shisheng AU - Cao S AD - Integrative Regeneration Laboratory, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, China. AD - State Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 301600, China. AD - Tianjin Key Laboratory of Neuromodulation and Neurorepair, Tianjin, 300192, China. FAU - Chen, Yongqiang AU - Chen Y AD - Integrative Regeneration Laboratory, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, China. AD - State Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 301600, China. AD - Tianjin Key Laboratory of Neuromodulation and Neurorepair, Tianjin, 300192, China. FAU - Li, Jinze AU - Li J AD - Integrative Regeneration Laboratory, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, China. AD - State Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 301600, China. AD - Tianjin Key Laboratory of Neuromodulation and Neurorepair, Tianjin, 300192, China. FAU - Li, Zixing AU - Li Z AD - Integrative Regeneration Laboratory, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, China. AD - State Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 301600, China. AD - Tianjin Key Laboratory of Neuromodulation and Neurorepair, Tianjin, 300192, China. FAU - Liu, Dazhuang AU - Liu D AD - Integrative Regeneration Laboratory, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, China. AD - State Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 301600, China. AD - Tianjin Key Laboratory of Neuromodulation and Neurorepair, Tianjin, 300192, China. FAU - Zhong, Langdi AU - Zhong L AD - Integrative Regeneration Laboratory, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, China. AD - State Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 301600, China. AD - Tianjin Key Laboratory of Neuromodulation and Neurorepair, Tianjin, 300192, China. FAU - Dong, Xiaoxi AU - Dong X AD - Integrative Regeneration Laboratory, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, China. dongxx@bme.cams.cn. AD - State Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 301600, China. dongxx@bme.cams.cn. AD - Tianjin Key Laboratory of Neuromodulation and Neurorepair, Tianjin, 300192, China. dongxx@bme.cams.cn. FAU - Dai, Jianwu AU - Dai J AD - Integrative Regeneration Laboratory, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, China. jwdai@genetics.ac.cn. AD - State Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 301600, China. jwdai@genetics.ac.cn. AD - Tianjin Key Laboratory of Neuromodulation and Neurorepair, Tianjin, 300192, China. jwdai@genetics.ac.cn. FAU - Yin, Huijuan AU - Yin H AUID- ORCID: 0000-0002-4821-7562 AD - Integrative Regeneration Laboratory, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, China. yinhj@bme.pumc.edu.cn. AD - State Key Laboratory of Advanced Medical Materials and Devices, Institute of Biomedical Engineering, Tianjin Institutes of Health Science, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin, 301600, China. yinhj@bme.pumc.edu.cn. AD - Tianjin Key Laboratory of Neuromodulation and Neurorepair, Tianjin, 300192, China. yinhj@bme.pumc.edu.cn. LA - eng GR - 2023-I2M-2-008/Chinese Academy of Medical Sciences Initiative for Innovative Medicine/ GR - 2023YFB3609103/National Key Research and Development Program of China/ GR - 62175261/National Natural Science Foundation of China/ PT - Journal Article DEP - 20260731 PL - England TA - J Transl Med JT - Journal of translational medicine JID - 101190741 RN - 0 (Presenilin-1) RN - 0 (Amyloid beta-Protein Precursor) SB - IM MH - Animals MH - *Low-Level Light Therapy MH - *Cognition/radiation effects MH - Monte Carlo Method MH - Mice, Transgenic MH - *Radiometry MH - Alzheimer Disease/physiopathology/pathology/radiotherapy MH - *Presenilin-1/metabolism MH - Brain/pathology/radiation effects/physiopathology MH - Mice MH - Calibration MH - Amyloid beta-Protein Precursor/metabolism OTO - NOTNLM OT - APPswe/PS1dE9 mouse model OT - Alzheimer's disease OT - Monte Carlo light transport modeling OT - Photobiomodulation OT - Target-area dosimetry-guided PBM COIS- Declarations. Ethics approval and consent to participate: All animal work was approved by the animal ethical and welfare committee at the Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College (IRM-DWLL-2022240) and performed with strict adherence to the guidelines set out in the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:05 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/02/03 00:00 [received] PHST- 2026/07/25 00:00 [accepted] PHST- 2026/10/30 01:05 [entrez] AID - 10.1186/s12967-026-08734-6 [pii] AID - 10.1186/s12967-026-08734-6 [doi] PST - epublish SO - J Transl Med. 2026 Jul 31;24(1):1368. doi: 10.1186/s12967-026-08734-6. PMID- 42958400 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1746-1596 (Electronic) IS - 1746-1596 (Linking) VI - 21 IP - 1 DP - 2026 Aug 1 TI - HER2-low and HER2-ultralow breast cancer in the antibody-drug conjugate era: diagnostic challenges and reporting workflow. LID - 79 [pii] LID - 10.1186/s13000-026-01822-7 [doi] AB - BACKGROUND: Human epidermal growth factor receptor 2 (HER2)-directed antibody-drug conjugates (ADCs) have expanded the clinical relevance of HER2 expression beyond the traditional HER2-positive versus HER2-negative framework. HER2-low breast cancer, commonly defined as immunohistochemistry (IHC) 1 + or IHC 2 + with negative in situ hybridization (ISH), is now treatment-relevant in selected advanced-disease settings. HER2-ultralow disease, characterized as IHC 0 with membrane staining below the IHC 1 + threshold, further challenges routine pathological interpretation. MAIN BODY: This pathology-centered review summarizes the evolution of HER2 classification from binary status to a clinically relevant expression continuum and clarifies the definitions of true HER2-zero, HER2-ultralow, HER2-low, and HER2-positive disease. Particular emphasis is placed on the zero/ultralow/1 + boundaries, reflex ISH or fluorescence in situ hybridization (FISH) testing for IHC 2 + cases, assay and fixation variables, nonspecific staining, intratumoral heterogeneity, specimen discordance, and interobserver variability. DESTINY-Breast06 and subsequent regional approvals have made HER2-ultralow actionable in defined hormone receptor-positive advanced-disease settings. A practical workflow is proposed for assessment, quality control, consensus review, and reporting. CONCLUSIONS: HER2-low and HER2-ultralow should be interpreted as clinically relevant extensions of established HER2 testing rather than as simple new labels. Responsible implementation requires standardized IHC scoring, careful morphological correlation, validated assays, transparent reporting language, and multidisciplinary communication. A reproducible pathology-centered workflow is essential for translating ADC-era advances into precise and equitable breast cancer care. CI - (c) 2026. The Author(s). FAU - Liang, Kuan AU - Liang K AUID- ORCID: 0009-0005-2675-2862 AD - Department of Pathology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China. AD - Shenzhen University, Shenzhen, China. FAU - Jia, Ziang AU - Jia Z AUID- ORCID: 0009-0002-6962-2433 AD - Shenzhen University, Shenzhen, China. FAU - Lu, Haiwen AU - Lu H AUID- ORCID: 0009-0009-9995-0914 AD - Department of Pathology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China. AD - Shenzhen University, Shenzhen, China. FAU - Yan, Ziling AU - Yan Z AUID- ORCID: 0009-0002-4961-7418 AD - Department of Pathology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China. FAU - Huang, Haihui AU - Huang H AUID- ORCID: 0009-0005-5546-9919 AD - Department of Pathology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China. FAU - Liu, Xia AU - Liu X AUID- ORCID: 0009-0007-6727-0688 AD - Department of Pathology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, China. mdcg2014bl@163.com. LA - eng GR - SZSM202411003/Sanming Project of Medicine in Shenzhen/ PT - Journal Article PT - Review DEP - 20260801 PL - England TA - Diagn Pathol JT - Diagnostic pathology JID - 101251558 RN - EC 2.7.10.1 (Erb-b2 Receptor Tyrosine Kinases) RN - EC 2.7.10.1 (ERBB2 protein, human) RN - 0 (Immunoconjugates) RN - 0 (Biomarkers, Tumor) RN - 0 (Antineoplastic Agents, Immunological) SB - IM MH - Humans MH - *Breast Neoplasms/pathology/drug therapy/diagnosis MH - Female MH - *Erb-b2 Receptor Tyrosine Kinases/metabolism/analysis MH - Immunohistochemistry MH - *Immunoconjugates/therapeutic use MH - In Situ Hybridization, Fluorescence MH - *Biomarkers, Tumor/analysis/metabolism MH - Workflow MH - Predictive Value of Tests MH - Antineoplastic Agents, Immunological/therapeutic use OTO - NOTNLM OT - Breast cancer OT - HER2-low OT - HER2-ultralow OT - Immunohistochemistry OT - Pathology reporting OT - Trastuzumab deruxtecan COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:05 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/06/07 00:00 [received] PHST- 2026/07/30 00:00 [accepted] PHST- 2026/10/30 01:05 [entrez] AID - 10.1186/s13000-026-01822-7 [pii] AID - 10.1186/s13000-026-01822-7 [doi] PST - epublish SO - Diagn Pathol. 2026 Aug 1;21(1):79. doi: 10.1186/s13000-026-01822-7. PMID- 42958398 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1749-8090 (Electronic) IS - 1749-8090 (Linking) VI - 21 IP - 1 DP - 2026 Jul 31 TI - Vacuum-assisted laser ablation induces pulmonary cavity formation: insights from a rabbit model. LID - 861 [pii] LID - 10.1186/s13019-026-04377-2 [doi] AB - BACKGROUND: Pulmonary nodules are common findings in low‑dose CT screening, and thermal ablation is a minimally invasive treatment option. However, incomplete ablation and tissue fragment dispersion remain challenges. METHODS: In this rabbit model study, laser ablation and microwave ablation were performed at three energy settings (600 J, 1,200 J, and 1,800 J) under CT guidance. An innovative three‑way vacuum adapter was integrated with the puncture needle to apply negative pressure suction during ablation. Gross pathology, lesion dimensions, cavity formation, and histological features of aspirated tissue were assessed. RESULTS: Laser ablation consistently produced larger lesion site rhombus areas than microwave ablation at equivalent energy levels (e.g., 87.2 mm(2) vs. 53.1 mm(2) at 600 J; 179.2 mm(2) vs. 104.6 mm(2) at 1,800 J). Cavity formation occurred exclusively in laser groups, while none was observed in microwave groups. Aspirated tissue fragments collected during laser ablation contained alveolar epithelial cells and lymphocytes, confirming effective vacuum‑assisted extraction. Histology revealed concentric pathological zones with increasing energy. DISCUSSION: The findings highlight the distinct advantages of vacuum‑assisted laser ablation over microwave ablation in pulmonary nodule treatment. Laser ablation with vacuum assistance not only produced larger lesion areas but also uniquely induced consistent cavity formation, which may serve as a marker of complete tumor eradication in various organ systems. Further experiment on larger animal models are warranted. CONCLUSIONS: Laser ablation combined with vacuum assistance achieved more efficient ablation, larger lesions, and consistent cavity formation compared to microwave ablation. This approach may enhance tumor eradication and warrants further investigation for application to other organ sites. CI - (c) 2026. The Author(s). FAU - Liu, Lijun AU - Liu L AD - Hebei General Hospital, No. 348 Heping West Road, Xinhua District, Shijiazhuang City, 050051, Hebei Province, P. R. China. 13833131067@163.com. FAU - Li, Xiaoning AU - Li X AD - Hebei General Hospital, No. 348 Heping West Road, Xinhua District, Shijiazhuang City, 050051, Hebei Province, P. R. China. FAU - Yu, Hang AU - Yu H AD - Hebei General Hospital, No. 348 Heping West Road, Xinhua District, Shijiazhuang City, 050051, Hebei Province, P. R. China. FAU - Wang, Jianxun AU - Wang J AD - Hebei General Hospital, No. 348 Heping West Road, Xinhua District, Shijiazhuang City, 050051, Hebei Province, P. R. China. LA - eng PT - Journal Article DEP - 20260731 PL - England TA - J Cardiothorac Surg JT - Journal of cardiothoracic surgery JID - 101265113 SB - IM MH - Animals MH - Rabbits MH - *Laser Therapy/methods/adverse effects MH - Vacuum MH - Microwaves/therapeutic use MH - *Lung Neoplasms/surgery/pathology MH - Disease Models, Animal MH - Tomography, X-Ray Computed MH - *Lung/pathology/surgery MH - *Solitary Pulmonary Nodule/surgery/pathology/diagnostic imaging COIS- Declarations. Ethics approval and consent to participate: The animal experiment was reviewed and approved by the Institutional Animal Care and Use Committee of Hebei General Hospital (2024 Research Ethics Approval No. 67). Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:04 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/01/26 00:00 [received] PHST- 2026/05/28 00:00 [accepted] PHST- 2026/10/30 01:04 [entrez] AID - 10.1186/s13019-026-04377-2 [pii] AID - 10.1186/s13019-026-04377-2 [doi] PST - epublish SO - J Cardiothorac Surg. 2026 Jul 31;21(1):861. doi: 10.1186/s13019-026-04377-2. PMID- 42958397 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 1746-4811 (Print) IS - 1746-4811 (Linking) VI - 22 IP - 1 DP - 2026 Jul 31 TI - CDScompare: a frame-sensitive GFF comparison tool for improved detection of coding sequence discrepancies, with applications to wheat and rice annotations. LID - 95 [pii] LID - 10.1186/s13007-026-01576-9 [doi] AB - BACKGROUND: The rapid expansion of high-quality plant genome assemblies, driven by advances in sequencing technologies and cost reductions, has led to a proliferation of genome assemblies. Moreover, for a single genome, multiple annotation sources often coexist: full-genome predictions from dedicated pipelines (sometimes split into high- and low-confidence sets), manually curated annotations focused on particular gene families, and independent annotations from public repositories such as NCBI. Accurately comparing these alternative annotations is essential to identify and resolve discrepancies, either by selecting the best gene model or, at minimum, by flagging conflicting gene structures at the same locus. Existing comparison tools can detect differences such as gene overlap, fusion or splitting, and some can also detect differences in coding/non-coding boundaries. However, when it comes to coding sequences, none of these tools take the reading frame into account despite its crucial role in interpreting protein-coding regions. This limits their ability to detect functionally relevant annotation conflicts, since the same nucleotide segment, when read in a different frame, yields a completely different protein. RESULTS: We present CDScompare, a Python-based tool that performs frame-aware comparison of GFF annotation files with a focus on CDS regions. This is valuable not only for researchers involved in genome annotation, but also for the many scientists working with gene families of interest, who often face conflicting annotations across multiple sources. As case studies, we applied CDScompare to compare (i) two public annotations of the hexaploid bread wheat (Triticum aestivum), and (ii) two annotations of rice (Oryza sativa) cv. Azucena. CDScompare revealed many CDS conflicts overlooked by existing tools, including cases where overlapping or identical CDS boundaries result in completely different protein sequences due to reading frame differences. Despite the size of the wheat GFF input files (> 1 GB in total), CDScompare processed the comparison in under one minute on a standard laptop, thanks to several algorithmic optimizations. CONCLUSIONS: CDScompare introduces a necessary layer of precision in structural annotation comparison by incorporating reading-frame awareness. It enables more informed decisions for annotation pipeline comparison and supports downstream analyses where functional annotation integrity is critical. CDScompare is open source and freely available. CI - (c) 2026. The Author(s). FAU - Ranwez, Vincent AU - Ranwez V AD - UMR AGAP Institut, Univ Montpellier, CIRAD, INRAE, Institut Agro, Montpellier, 34398, France. vincent.ranwez@supagro.fr. FAU - Girodolle, Johanna AU - Girodolle J AD - UMR AGAP Institut, Univ Montpellier, CIRAD, INRAE, Institut Agro, Montpellier, 34398, France. FAU - Jacot, Vetea AU - Jacot V AD - UMR AGAP Institut, Univ Montpellier, CIRAD, INRAE, Institut Agro, Montpellier, 34398, France. FAU - Chantret, Nathalie AU - Chantret N AD - UMR AGAP Institut, Univ Montpellier, CIRAD, INRAE, Institut Agro, Montpellier, 34398, France. LA - eng GR - ANR-24-CE20-2538/French Agence Nationale de la Recherche/ GR - 101134965/European program (HORIZON-CL6)/ PT - Journal Article DEP - 20260731 PL - England TA - Plant Methods JT - Plant methods JID - 101245798 OTO - NOTNLM OT - Bioinformatics tool OT - Coding sequence (CDS) OT - Frameshift OT - Reading frame OT - Rice (Oryza sativa) OT - Structural annotation comparison OT - Wheat (Triticum aestivum) COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:04 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/02/23 00:00 [received] PHST- 2026/07/10 00:00 [accepted] PHST- 2026/10/30 01:04 [entrez] AID - 10.1186/s13007-026-01576-9 [pii] AID - 10.1186/s13007-026-01576-9 [doi] PST - epublish SO - Plant Methods. 2026 Jul 31;22(1):95. doi: 10.1186/s13007-026-01576-9. PMID- 42958395 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1746-4269 (Electronic) IS - 1746-4269 (Linking) VI - 22 IP - 1 DP - 2026 Jul 31 TI - Temple prescription slips as living ethnomedicine: practitioner knowledge and exploratory bioinformatics mapping of Malaysian Chinese folk therapeutics. LID - 99 [pii] LID - 10.1186/s13002-026-00914-4 [doi] AB - BACKGROUND: Temple prescription slips are Chinese religious-medical texts that combine divination practice, materia medica knowledge and household self-care. Malaysian Chinese temple slips remain poorly documented, particularly with respect to sampling, transcription, source identification, safety issues and reproducible informatics analysis. METHODS: A complete on-site convenience census of 106 publicly accessible prescription slips from the Goddess of Mercy Temple, Penang, was photographed. One non-prescription slip and two illegible slips were excluded, leaving 103 prescriptions for practitioner-based analysis. Herb names were transcribed, standardised, interpreted with an experienced TCM practitioner and cross-checked against materia medica, pharmacopoeial and plant-name resources. Prescriptions were categorised into Formulary of Chinese Medicine categories, coded for safety/interpretation issues and mapped using BATMAN-TCM disease-domain enrichment (target score > = 0.84; adjusted p < 0.05). The workflow was designed for documentary standardisation and hypothesis generation rather than validation of clinical efficacy. RESULTS: The most frequent categories were Tonifying (16/103, 15.53%), Heat-clearing (15/103, 14.56%), Phlegm-resolving (10/103, 9.71%) and Dampness-eliminating (9/103, 8.74%). Several entries required correction or remained low-confidence because of handwritten, vernacular or multi-source names. Practitioner-coded cautions were dominated by cold-digestion, yin-deficiency/heat-dry, dampness/phlegm/stagnation, pregnancy/reproductive and toxic/mineral safety issues. BATMAN-TCM generated broad enrichment outputs for 101 prescriptions, mainly cardiovascular, pain/inflammatory and neurodegenerative/neurological domains. These outputs were treated as exploratory database signals, not clinical claims. CONCLUSION: This single-site corpus shows recognisable TCM formula logic, diaspora adaptation and safety-relevant uncertainties. The findings are hypothesis-generating only; they do not validate temple medicine generally or demonstrate efficacy for specific diseases. The protocol provides a reproducible basis for future multi-site, multi-practitioner, pharmacognostic and experimental research. CI - (c) 2026. The Author(s). FAU - Chong, Jia Yi AU - Chong JY AD - The Second Affiliated Hospital of Henan Medical University (Henan Mental Hospital), Xinxiang City, 453002, Henan Province, China. AD - School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, 11800, Penang, Malaysia. FAU - Ooi, Pin Jun AU - Ooi PJ AD - The Second Affiliated Hospital of Henan Medical University (Henan Mental Hospital), Xinxiang City, 453002, Henan Province, China. AD - School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, 11800, Penang, Malaysia. AD - Faculty of Business and Communications, INTI International University, Persiaran Perdana BBN, 71800, Nilai, Malaysia. FAU - Ang, Xuan Jie AU - Ang XJ AD - School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, 11800, Penang, Malaysia. FAU - Wong, Jing Hui AU - Wong JH AD - School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, 11800, Penang, Malaysia. AD - Faculty of Business and Communications, INTI International University, Persiaran Perdana BBN, 71800, Nilai, Malaysia. FAU - Ang, Alvin Chin Ping AU - Ang ACP AD - School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, 11800, Penang, Malaysia. FAU - Qiu, Qiyue AU - Qiu Q AD - The Second Affiliated Hospital of Henan Medical University (Henan Mental Hospital), Xinxiang City, 453002, Henan Province, China. drqiyueqiu@163.com. AD - School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, 11800, Penang, Malaysia. drqiyueqiu@163.com. FAU - Zhang, Ruiling AU - Zhang R AD - The Second Affiliated Hospital of Henan Medical University (Henan Mental Hospital), Xinxiang City, 453002, Henan Province, China. zhangruilingxxmu@126.com. FAU - Yam, Mun Fei AU - Yam MF AD - The Second Affiliated Hospital of Henan Medical University (Henan Mental Hospital), Xinxiang City, 453002, Henan Province, China. yammunfei@yahoo.com. AD - School of Pharmaceutical Sciences, Universiti Sains Malaysia, Minden, 11800, Penang, Malaysia. yammunfei@yahoo.com. AD - Faculty of Business and Communications, INTI International University, Persiaran Perdana BBN, 71800, Nilai, Malaysia. yammunfei@yahoo.com. LA - eng PT - Journal Article DEP - 20260731 PL - England TA - J Ethnobiol Ethnomed JT - Journal of ethnobiology and ethnomedicine JID - 101245794 SB - IM MH - *Medicine, Chinese Traditional MH - Humans MH - Malaysia MH - *Computational Biology MH - Traditional Medicine Practitioners OTO - NOTNLM OT - BATMAN-TCM OT - Network pharmacology OT - Temple prescription OT - Traditional chinese medicine COIS- Declarations. Ethics approval and consent to participate: Not applicable. This study involved non-invasive documentation of publicly accessible temple prescription slips and professional consultation with an experienced TCM practitioner, who provided verbal informed consent for anonymised use of their input. No patient, devotee or personal health information was collected. The study followed standard documentary and ethnographic ethics principles, including voluntary participation, anonymisation, respect for the religious site and non-collection of clinical data. Consent for publication: Consent for publication was obtained from the participating TCM practitioner for the anonymized use of their insights. The authors consent to publication of this manuscript. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:04 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/02/13 00:00 [received] PHST- 2026/05/29 00:00 [accepted] PHST- 2026/10/30 01:04 [entrez] AID - 10.1186/s13002-026-00914-4 [pii] AID - 10.1186/s13002-026-00914-4 [doi] PST - epublish SO - J Ethnobiol Ethnomed. 2026 Jul 31;22(1):99. doi: 10.1186/s13002-026-00914-4. PMID- 42958393 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1479-5876 (Electronic) IS - 1479-5876 (Linking) VI - 24 IP - 1 DP - 2026 Aug 20 TI - Platelet-derived mitochondrial transplantation expands respiratory reserve and extracellular matrix anabolism in primary osteoarthritic chondrocytes. LID - 1363 [pii] LID - 10.1186/s12967-026-08809-4 [doi] AB - BACKGROUND: Osteoarthritis (OA) is a leading cause of disability worldwide, driven by the complex interplay of aging, obesity, and mechanical stress, which converge on progressive deterioration of cartilage, subchondral bone, and synovial tissue. Current treatments alleviate symptoms but fail to halt the degenerative process, making joint replacement surgery the only definitive option for advanced disease. A central mechanism underlying OA pathogenesis is mitochondrial dysfunction in chondrocytes, in which the balance between oxidative phosphorylation (OXPHOS) and glycolysis is disrupted, leading to impaired OXPHOS, accumulation of reactive oxygen species (ROS), and sustained overproduction of matrix-degrading enzymes and pro-inflammatory cytokines. Mitochondrial transplantation has emerged as a promising strategy to restore cellular bioenergetics, and platelets, which contain functional, respiration-competent mitochondria, represent an accessible allogenic donor source. METHODS: Mitochondria isolated from platelets of healthy donors were structurally and functionally characterized and transplanted into primary human OA chondrocytes by mitoception at increasing chondrocyte-to-mitochondria ratios. Internalization was assessed by flow cytometry and confocal colocalization analysis. Bioenergetic effects were quantified by Seahorse extracellular flux analysis; mitochondrial membrane potential and superoxide production were measured by flow cytometry; extracellular matrix protein expression was evaluated by immunofluorescence and RT-qPCR; and the inflammatory secretome was profiled by Luminex multiplex assay. RESULTS: Isolated platelet mitochondria preserved outer membrane integrity, membrane potential, and ADP-coupled respiratory activity prior to transplantation. They were efficiently internalized by OA chondrocytes in a dose-dependent manner, with optimal uptake observed at chondrocyte-to-platelet-equivalent mitochondria dose ratios of 1:100. Transplantation increased maximal respiratory capacity and spare respiratory reserve without altering basal respiration, was accompanied by elevated type II collagen and aggrecan protein levels, and selectively reduced secreted IL-1beta and osteopontin while leaving IL-6, TNF-alpha, IL-10, and IL-1RA unchanged. Mitochondrial superoxide production was modestly increased and was prevented by the mitochondria-targeted antioxidant MitoTEMPO, while mitochondrial membrane potential remained unchanged. CONCLUSIONS: Platelet-derived mitochondrial transplantation expands the respiratory reserve and modulates the catabolic-anabolic balance of human OA chondrocytes without inducing mitochondrial depolarization, providing a mechanistic basis for allogenic, blood-derived mitochondrial therapies in joint disease. CI - (c) 2026. The Author(s). FAU - Jeraldo-Araya, Nicole AU - Jeraldo-Araya N AD - Centro de Investigacion e Innovacion Biomedica, Facultad de Medicina, Universidad de Los Andes, Santiago, 7620001, Chile. AD - Centro de Investigacion Clinica Avanzada, Hospital Clinico Universidad de Chile, Santiago, 8380453, Chile. FAU - Yanten-Fuentes, Liliana AU - Yanten-Fuentes L AD - Centro de Investigacion e Innovacion Biomedica, Facultad de Medicina, Universidad de Los Andes, Santiago, 7620001, Chile. AD - IMPACT, Center of Interventional Medicine for Precision and Advanced Cellular Therapy, Santiago, 7620001, Chile. FAU - Hernandez-Caceres, Maria Paz AU - Hernandez-Caceres MP AD - Centro de Investigacion e Innovacion Biomedica, Facultad de Medicina, Universidad de Los Andes, Santiago, 7620001, Chile. AD - IMPACT, Center of Interventional Medicine for Precision and Advanced Cellular Therapy, Santiago, 7620001, Chile. FAU - Morales-Reyes, Jonathan AU - Morales-Reyes J AD - Centro de Investigacion e Innovacion Biomedica, Facultad de Medicina, Universidad de Los Andes, Santiago, 7620001, Chile. AD - IMPACT, Center of Interventional Medicine for Precision and Advanced Cellular Therapy, Santiago, 7620001, Chile. FAU - Vega-Letter, Ana Maria AU - Vega-Letter AM AD - Escuela de Ingenieria Bioquimica, Pontificia Universidad Catolica de Valparaiso, Valparaiso, 2340000, Chile. FAU - Lara-Barba, Eliana AU - Lara-Barba E AD - Centro de Investigacion e Innovacion Biomedica, Facultad de Medicina, Universidad de Los Andes, Santiago, 7620001, Chile. AD - IMPACT, Center of Interventional Medicine for Precision and Advanced Cellular Therapy, Santiago, 7620001, Chile. FAU - Merino-Flores, Cesar AU - Merino-Flores C AD - Centro de Investigacion e Innovacion Biomedica, Facultad de Medicina, Universidad de Los Andes, Santiago, 7620001, Chile. AD - IMPACT, Center of Interventional Medicine for Precision and Advanced Cellular Therapy, Santiago, 7620001, Chile. FAU - Bustamante-Barrientos, Felipe A AU - Bustamante-Barrientos FA AD - Centro de Investigacion e Innovacion Biomedica, Facultad de Medicina, Universidad de Los Andes, Santiago, 7620001, Chile. AD - IMPACT, Center of Interventional Medicine for Precision and Advanced Cellular Therapy, Santiago, 7620001, Chile. AD - Escuela de Kinesiologia, Facultad de Medicina, Universidad de Los Andes, Santiago, 7620001, Chile. FAU - Alfaro, Ivan AU - Alfaro I AD - Instituto de Ciencias e Innovacion en Medicina, Facultad de Medicina, Universidad del Desarrollo, Santiago, 7610315, Chile. AD - Centro Ciencia y Vida, Fundacion Ciencia y Vida, 8580704 , Santiago, Chile. FAU - Matamoros, Andrea AU - Matamoros A AD - Instituto de Ciencias Biomedicas, Facultad de Medicina y Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, 8370071, Chile. FAU - Elorza, Alvaro A AU - Elorza AA AD - Instituto de Ciencias Biomedicas, Facultad de Medicina y Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, 8370071, Chile. FAU - Garcia-Navarrete, Camila AU - Garcia-Navarrete C AD - Centro de Investigacion Clinica Avanzada, Hospital Clinico Universidad de Chile, Santiago, 8380453, Chile. AD - Centre for Organismal Studies (COS), Cytoskeleton, Cell division and Signaling Unit, University of Heidelberg, 69120, Heidelberg, Germany. FAU - Luque-Campos, Noymar AU - Luque-Campos N AD - IRMB, Univ Montpellier, INSERM, Montpellier, 34295, France. FAU - Barahona, Maximiliano AU - Barahona M AD - Centro de Investigacion Clinica Avanzada, Hospital Clinico Universidad de Chile, Santiago, 8380453, Chile. AD - Departamento de Ortopedia y Traumatologia, Hospital Clinico Universidad de Chile, Santiago, 8380453, Chile. FAU - Altamirano, Claudia AU - Altamirano C AD - Escuela de Ingenieria Bioquimica, Pontificia Universidad Catolica de Valparaiso, Valparaiso, 2340000, Chile. FAU - Luz-Crawford, Patricia AU - Luz-Crawford P AD - Centro de Investigacion e Innovacion Biomedica, Facultad de Medicina, Universidad de Los Andes, Santiago, 7620001, Chile. pluz@uandes.cl. AD - IMPACT, Center of Interventional Medicine for Precision and Advanced Cellular Therapy, Santiago, 7620001, Chile. pluz@uandes.cl. FAU - Cruz, Pablo AU - Cruz P AD - Centro de Investigacion e Innovacion Biomedica, Facultad de Medicina, Universidad de Los Andes, Santiago, 7620001, Chile. pablo170693@uchile.cl. AD - Departamento de Tecnologia Medica, Facultad de Medicina, Universidad de Chile, Santiago, 8380453, Chile. pablo170693@uchile.cl. AD - Centro de Investigacion Clinica Avanzada, Hospital Clinico Universidad de Chile, Santiago, 8380453, Chile. pablo170693@uchile.cl. FAU - Toledo, Jorge A AU - Toledo JA AUID- ORCID: 0000-0001-8678-558X AD - Centro de Investigacion Clinica Avanzada, Hospital Clinico Universidad de Chile, Santiago, 8380453, Chile. jorge.toledo@uaysen.cl. AD - Departamento de Ciencias de la Salud, Universidad de Aysen, Coyhaique, 5951537, Chile. jorge.toledo@uaysen.cl. LA - eng GR - FONDECYT Regular 1250932/Agencia Nacional de Investigacion y Desarrollo/ GR - FONDEF TA24I005/Agencia Nacional de Investigacion y Desarrollo/ GR - FONDEQUIP EQM220115/Agencia Nacional de Investigacion y Desarrollo/ GR - FONDEQUIP EQM230139/Agencia Nacional de Investigacion y Desarrollo/ GR - #FB210024/Agencia Nacional de Investigacion y Desarrollo/ GR - 11251364/Agencia Nacional de Investigacion y Desarrollo/ GR - 11261321/Agencia Nacional de Investigacion y Desarrollo/ GR - 3250680/Agencia Nacional de Investigacion y Desarrollo/ GR - 21263842/Agencia Nacional de Investigacion y Desarrollo/ GR - EQY230019/Agencia Nacional de Investigacion y Desarrollo/ PT - Journal Article DEP - 20260820 PL - England TA - J Transl Med JT - Journal of translational medicine JID - 101190741 SB - IM MH - Humans MH - *Chondrocytes/metabolism/pathology MH - *Mitochondria/transplantation/metabolism/ultrastructure MH - *Osteoarthritis/pathology/metabolism/therapy MH - *Blood Platelets/metabolism MH - *Extracellular Matrix/metabolism MH - Cell Respiration MH - Membrane Potential, Mitochondrial OTO - NOTNLM OT - Chondrocyte bioenergetics OT - Extracellular matrix remodeling OT - Mitochondrial transplantation OT - Osteoarthritis OT - Platelet-derived mitochondria COIS- Declarations. Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:04 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/05/04 00:00 [received] PHST- 2026/08/09 00:00 [accepted] PHST- 2026/10/30 01:04 [entrez] AID - 10.1186/s12967-026-08809-4 [pii] AID - 10.1186/s12967-026-08809-4 [doi] PST - epublish SO - J Transl Med. 2026 Aug 20;24(1):1363. doi: 10.1186/s12967-026-08809-4. PMID- 42958387 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1479-5876 (Electronic) IS - 1479-5876 (Linking) VI - 24 IP - 1 DP - 2026 Aug 3 TI - Methionine addiction in oral squamous cell carcinoma: a metabolic vulnerability targeted by engineered bacteria. LID - 1373 [pii] LID - 10.1186/s12967-026-08710-0 [doi] AB - Metabolic reprogramming is a defining hallmark of malignant transformation, and tumor-specific amino acid addiction has emerged as a promising therapeutic axis for precision oncology. Oral squamous cell carcinoma (OSCC) is an aggressive head and neck malignancy marked by limited curative options, frequent recurrence, and poor long-term survival. Cumulative multi-omics and clinical evidence consistently confirm widespread methionine addiction across OSCC lesions, yet conventional systemic methionine restriction strategies are hampered by severe off-target organ toxicity and insufficient tumor selectivity. Engineered attenuated Salmonella typhimurium strain SGN1 represents a tumor microenvironment-homing live biotherapeutic platform that achieves localized intratumoral methionine degradation with minimal systemic perturbation. Preclinical data across immunocompetent murine models and patient-derived organoids demonstrate robust anti-OSCC activity alongside controllable antibiotic-mediated clearance and broad systemic biosafety. This Commentary contextualizes SGN1 within the broader landscape of microbe-mediated amino acid depletion therapies, systematically dissects the core metabolic mechanisms underpinning OSCC methionine addiction, elaborates preclinical efficacy and safety profiles of SGN1, expands critical discussion of translational bottlenecks, and outlines structured clinical development frameworks for live bacterial metabolic therapeutics. By integrating comparative analysis of arginine and tryptophan-targeted microbial interventions, this work provides balanced, forward-looking insights to advance stratified, minimally invasive precision treatment for methionine-addicted OSCC and other solid tumors. CI - (c) 2026. The Author(s). FAU - Li, Chenxi AU - Li C AUID- ORCID: 0000-0003-1443-4769 AD - Cancer Research Institute, The Third Teaching Hospital, The Affiliated Cancer Hospital of Xinjiang Medical University, Xinjiang Key Laboratory of Translational Biomedical Engineering, No. 789 Soochow East Street, Urumqi, 830011, China. lichenxi@xjmu.edu.cn. AD - Department of Oral and Maxillofacial Oncology & Surgery, School / Hospital of Stomatology, the First Affiliated Hospital of Xinjiang Medical University, National Clinical Medical Research Institute, Stomatological Research Institute of Xinjiang Uygur Autonomous Region, Urumqi, 830054, China. lichenxi@xjmu.edu.cn. AD - Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, 210029, China. lichenxi@xjmu.edu.cn. AD - Guangdong Provincial Key Laboratory of Oral Diseases, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, 510080, China. lichenxi@xjmu.edu.cn. FAU - Chen, Yan AU - Chen Y AD - Department of Preventive Dentistry, School / Hospital of Stomatology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830054, China. FAU - Wang, Zhengye AU - Wang Z AD - Division of Cancer Research and Clinical Oncology, Center for Disease Control and Prevention of Xinjiang Production and Construction Corps, Urumqi, 830092, China. FAU - Gong, Zhongcheng AU - Gong Z AD - Department of Oral and Maxillofacial Oncology & Surgery, School / Hospital of Stomatology, the First Affiliated Hospital of Xinjiang Medical University, National Clinical Medical Research Institute, Stomatological Research Institute of Xinjiang Uygur Autonomous Region, Urumqi, 830054, China. AD - College of General Practice, Xinjiang Medical University, Urumqi, 830017, China. FAU - Ni, Yuchen AU - Ni Y AD - Division of Medical Affairs, The Third Teaching Hospital, The Affiliated Cancer Hospital of Xinjiang Medical University, No. 789 Soochow East Street, Urumqi, 830011, China. niyuchen90@163.com. LA - eng GR - XJRC-2025-KJ-PY-KJLJ-118/the Xinjiang Talent Development Fund/ GR - 2025D01C175/Natural Science Foundation of Xinjiang Uygur Autonomous Region/ GR - GCZX2026-07/Jiangsu Province Engineering Research Center of Stomatological Translational Medicine/ GR - KF2025120102/Open Fund of Guangdong Provincial Key Laboratory of Stomatology/ GR - 2026E02065/Xinjiang Science and Technology Support Project/ PT - Journal Article DEP - 20260803 PL - England TA - J Transl Med JT - Journal of translational medicine JID - 101190741 RN - AE28F7PNPL (Methionine) SB - IM MH - Humans MH - *Methionine/metabolism MH - *Mouth Neoplasms/metabolism/pathology/microbiology MH - Animals MH - *Carcinoma, Squamous Cell/metabolism MH - *Salmonella typhimurium/metabolism MH - *Microorganisms, Genetically-Modified/metabolism OTO - NOTNLM OT - Engineered Salmonella OT - Live biotherapeutics OT - Methionine addiction OT - Oral squamous cell carcinoma OT - Patient-derived organoids OT - Tumor metabolic vulnerability COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:04 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/04/10 00:00 [received] PHST- 2026/07/22 00:00 [accepted] PHST- 2026/10/30 01:04 [entrez] AID - 10.1186/s12967-026-08710-0 [pii] AID - 10.1186/s12967-026-08710-0 [doi] PST - epublish SO - J Transl Med. 2026 Aug 3;24(1):1373. doi: 10.1186/s12967-026-08710-0. PMID- 42958366 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1601-5223 (Electronic) IS - 0018-0661 (Linking) VI - 163 IP - 1 DP - 2026 Aug 1 TI - Preliminary functional characterization of the LMNA c.929 A > G (p.Gln310Arg) variant identified in a patient with dilated cardiomyopathy. LID - 123 [pii] LID - 10.1186/s41065-026-00722-6 [doi] AB - BACKGROUND: Variants in LMNA are established causes of inherited dilated cardiomyopathy (DCM); however, the clinical significance of many rare missense variants remains uncertain. We identified the rare LMNA c.929 A > G (p.Gln310Arg) variant in a patient with progressive DCM and performed preliminary variant-specific functional characterization in an AC16 transient overexpression model. METHODS: Duo whole-exome sequencing and copy-number variation analysis were performed in the proband and his son. Wild-type and p.Gln310Arg lamin A expression constructs were transiently transfected into AC16 cells. Flag-tagged protein expression, Flag-based transfection efficiency, and lamin A/C distribution were assessed by western blotting and immunofluorescence staining. Mitochondrial membrane potential and intracellular ROS-associated fluorescence were assessed using JC-1 and DCFH-DA staining, respectively. ERK1/2, JNK, and p38 MAPK phosphorylation was evaluated by western blotting. Hypertrophy-associated NPPA and NPPB expression was measured by RT-qPCR, and cell area was quantified after phalloidin staining. RESULTS: The LMNA c.929 A > G variant was classified as a variant of uncertain significance. Wild-type and p.Gln310Arg lamin A constructs showed comparable Flag-tagged protein expression and Flag-based transfection efficiency. Expression of p.Gln310Arg lamin A was associated with an increased proportion of cells displaying ring-like lamin A/C staining patterns. Variant-expressing cells showed increased intracellular DCF fluorescence, reduced JC-1 red-to-green fluorescence ratio, and selectively increased ERK1/2 phosphorylation, whereas no significant changes were detected in JNK or p38 MAPK phosphorylation. The same cells also showed a hypertrophy-like phenotype characterized by increased NPPA and NPPB expression and enlarged cell area. CONCLUSION: In an AC16 transient overexpression model, expression of p.Gln310Arg lamin A was associated with altered lamin A/C distribution, increased intracellular ROS-associated fluorescence, reduced mitochondrial membrane potential, selectively increased ERK1/2 phosphorylation, and a hypertrophy-like cellular phenotype. These findings provide preliminary variant-specific functional evidence but do not establish the pathogenicity of the variant or the causal relationships among the observed cellular abnormalities. Further validation in endogenous and physiologically relevant cardac models is required. CI - (c) 2026. The Author(s). FAU - Han, Zikang AU - Han Z AD - Qingdao Medical College, Qingdao university, Qingdao, Shandong, China. AD - Department of Cardiology, Yantai Yuhuangding Hospital, Yantai, Shandong, China. FAU - Sheng, Yonghao AU - Sheng Y AD - Qingdao Medical College, Qingdao university, Qingdao, Shandong, China. AD - Department of Cardiology, Yantai Yuhuangding Hospital, Yantai, Shandong, China. FAU - Sun, Han AU - Sun H AD - Qingdao Medical College, Qingdao university, Qingdao, Shandong, China. AD - Department of Cardiology, Yantai Yuhuangding Hospital, Yantai, Shandong, China. FAU - Sun, Mengchen AU - Sun M AD - Qingdao Medical College, Qingdao university, Qingdao, Shandong, China. AD - Department of Cardiology, Yantai Yuhuangding Hospital, Yantai, Shandong, China. FAU - Dong, Haibin AU - Dong H AD - Department of Cardiology, Yantai Yuhuangding Hospital, Yantai, Shandong, China. FAU - Gong, Lei AU - Gong L AD - Department of Cardiology, Yantai Yuhuangding Hospital, Yantai, Shandong, China. FAU - Wang, Chunxiao AU - Wang C AD - Department of Cardiology, Yantai Yuhuangding Hospital, Yantai, Shandong, China. FAU - Wang, Guangqiang AU - Wang G AD - Department of Cardiology, Yantai Yuhuangding Hospital, Yantai, Shandong, China. wgq198632@126.com. FAU - Zhong, Lin AU - Zhong L AD - Department of Cardiology, Yantai Yuhuangding Hospital, Yantai, Shandong, China. yizun1971@126.com. LA - eng PT - Case Reports PT - Journal Article DEP - 20260801 PL - England TA - Hereditas JT - Hereditas JID - 0374654 RN - 0 (Lamin Type A) RN - 0 (LMNA protein, human) SB - IM MH - *Lamin Type A/genetics MH - *Cardiomyopathy, Dilated/genetics MH - Humans MH - Male MH - DNA Copy Number Variations MH - Exome Sequencing MH - Membrane Potential, Mitochondrial MH - Mutation, Missense MH - Cell Line OTO - NOTNLM OT - LMNA OT - Dilated cardiomyopathy OT - ERK1/2 OT - Lamin A/C OT - Variant of uncertain significance COIS- Declarations. Ethics approval and consent to participate: All procedures involving human participants were approved by the Ethics Committee of Yantai Yuhuangding Hospital (approval No. 2025 - 843) and were conducted in accordance with the approved protocol. Written informed consent was obtained from all participating individuals. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:03 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2025/12/30 00:00 [received] PHST- 2026/07/29 00:00 [accepted] PHST- 2026/10/30 01:03 [entrez] AID - 10.1186/s41065-026-00722-6 [pii] AID - 10.1186/s41065-026-00722-6 [doi] PST - epublish SO - Hereditas. 2026 Aug 1;163(1):123. doi: 10.1186/s41065-026-00722-6. PMID- 42958359 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2050-7283 (Electronic) IS - 2050-7283 (Linking) VI - 14 IP - 1 DP - 2026 Jul 24 TI - Body image, associated stigma as a determinant of depression, anxiety, and stress among adolescents in Salem, Tamil Nadu: a cross-sectional study. LID - 1567 [pii] LID - 10.1186/s40359-026-05222-w [doi] AB - BACKGROUND: Body image shaming is a growing public health concern affecting adolescent mental health. Adolescents face increasing pressure from peers, families, and the media regarding their appearance. Negative comments about appearance have been associated with depression, anxiety, and stress during this critical developmental stage. AIM: To explore the association between body image shaming and depression, anxiety, and stress among adolescents in Salem, Tamil Nadu. METHODS: A cross-sectional study was conducted among 310 adolescents aged 10-19 years, selected through multistage random sampling in Salem. Data were collected using a structured questionnaire including socio-demographics, the Body Image Shaming Scale (BISS-20), and the Depression, Anxiety, and Stress Scale (DASS-21). Inferential statistics included chi-square tests to examine associations between categorical variables and binary logistic regression analyses adjusted for gender, BMI category, residence, and education level to assess the independent association between body image shaming and depression, anxiety, and stress. RESULTS: High levels of body image shaming were reported by 46.8% of the participants. Mild depression (19.7%), extremely severe anxiety (22.6%), and moderate stress (19.7%) were observed. There was a significant association between body image shaming and depression (chi(2)=13.5; p = 0.009), anxiety (chi(2)=41.2; p < 0.001), and stress (chi(2)=36.78; p < 0.001). BMI was significantly associated with stress (p < 0.02). CONCLUSION: Body image shaming is prevalent among adolescents and is strongly associated with adverse mental health outcomes. Awareness campaigns should target both adolescents who experience shaming and those who engage in it, promoting empathy, body positivity, and supportive peer interactions. Targeted interventions, such as awareness campaigns, peer support groups, counselling services, and school-based programs, are essential to address this issue and promote adolescent well-being. CI - (c) 2026. The Author(s). FAU - P, Sandhya AU - P S AUID- ORCID: 0009-0008-2648-8047 AD - Master of Public Health, Department of Public Health, School of Allied Health Sciences, VIMS campus, VMRF-DU, Salem, Tamil Nadu, India. FAU - Krishnamoorthy, Priyanka AU - Krishnamoorthy P AUID- ORCID: 0000-0002-7602-9139 AD - Department of Public Health, School of Allied Health Sciences, VIMS Campus, VMRF - DU, Salem, Tamil Nadu, India. priyankakp96@gmail.com. FAU - Balasundaram, Sendilkumar AU - Balasundaram S AUID- ORCID: 0000-0001-5368-5603 AD - Dean, Allied Health Sciences, VIMS Campus, VMRF - DU, Salem, Tamil Nadu, India. FAU - Sambasivam, Indra AU - Sambasivam I AUID- ORCID: 0000-0002-5123-4427 AD - Department of Public Health, School of Allied Health Sciences, VIMS Campus, VMRF - DU, Salem, Tamil Nadu, India. LA - eng PT - Journal Article DEP - 20260724 PL - England TA - BMC Psychol JT - BMC psychology JID - 101627676 SB - IM MH - Humans MH - Adolescent MH - Cross-Sectional Studies MH - *Body Image/psychology MH - *Anxiety/psychology/epidemiology MH - *Depression/psychology/epidemiology MH - India/epidemiology MH - Female MH - Child MH - Male MH - *Stress, Psychological/psychology/epidemiology MH - Young Adult OTO - NOTNLM OT - Adolescents OT - Anxiety OT - Body image shaming OT - Depression OT - Mental health OT - Stress COIS- Declarations. Ethics approval and consent to participate: The study was approved by the Institutional Ethics Committee of Vinayaka Mission's Research Foundation - Deemed to be University (VMRF-DU), Salem, Tamil Nadu, India. Approval Number: VMKVMC&H/IEC/24/126. Written parental consent and adolescent assent were obtained for participants younger than 18 years, while informed consent was obtained directly from participants aged 18 years and above. Competing interests: The authors declare no competing interests. Conflict of interest: The authors declare that there is no conflict of interest. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:02 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/07 00:00 [received] PHST- 2026/07/20 00:00 [accepted] PHST- 2026/10/30 01:02 [entrez] AID - 10.1186/s40359-026-05222-w [pii] AID - 10.1186/s40359-026-05222-w [doi] PST - epublish SO - BMC Psychol. 2026 Jul 24;14(1):1567. doi: 10.1186/s40359-026-05222-w. PMID- 42958358 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1475-925X (Electronic) IS - 1475-925X (Linking) VI - 25 IP - 1 DP - 2026 Aug 2 TI - Computational analysis of a high-density electrode to scale the electric field geometry and intensity for neurostimulation. LID - 122 [pii] LID - 10.1186/s12938-026-01610-w [doi] AB - High-density grid electrodes can be configured to spatially scale the neurostimulation fields and enable the control of depth, intensity, and stimulation vectoring. In this study, we computationally analyze a high-density cluster electrode (HDCE) technology to measure its scalability and its effects on human tissue. The models are developed in Ansys using the Maxwell 3D Design modeler. More than 24 HDCE electrode configurations were analyzed and compared to standard 8 mm Ag/AgCl electrodes. It was found that the HDCE electrode configurations can be selected to emulate the electric field properties to within 1% of a standard 8 mm Ag/AgCl electrode. Moreover, increasing the number of active electrode contacts in the HDCE non-linearly increases tissue voltage and field intensity at various tissue depths. Specifically, it was found that the sensitivity of tissue voltage and field intensity decreases with the number of active HDCE electrode contacts. Similarly, the different spatial configurations of the HDCE can change the field shape by up to 54%, while the field intensity can be scaled by up to 186%. Similarly, the vector of the stimulation field (i.e., stimulation vector) through the tissues can be adjusted by shifting active electrode positions. When comparing these electrodes to standard 8 mm Ag/AgCl electrodes the results suggest a transformative impact of these technologies to optimize neurostimulation applications. The work presented here is part of a larger project aiming to develop next-generation scalable neurostimulation system that improve rehabilitation outcomes, and provide new adaptive solutions for personalized healthcare. CI - (c) 2026. The Author(s). FAU - Mancabelli, Kegan AU - Mancabelli K AD - Department of Biomedical Engineering, University at Buffalo, Buffalo, NY, 14260, USA. AD - Department of Biology, Cornell University, Ithaca, NY, 14850, USA. FAU - Titus, Albert H AU - Titus AH AD - Department of Biomedical Engineering, University at Buffalo, Buffalo, NY, 14260, USA. FAU - Stefanovic, Filip AU - Stefanovic F AD - Department of Biomedical Engineering, University at Buffalo, Buffalo, NY, 14260, USA. filipste@buffalo.edu. LA - eng GR - 2130651/Directorate for Biological Sciences/ PT - Journal Article DEP - 20260802 PL - England TA - Biomed Eng Online JT - Biomedical engineering online JID - 101147518 SB - IM MH - Electrodes MH - Humans MH - *Electric Stimulation/instrumentation MH - *Electricity MH - *Computer Simulation COIS- Declarations. Ethics approval and consent to participate: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:02 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/18 00:00 [received] PHST- 2026/07/21 00:00 [accepted] PHST- 2026/10/30 01:02 [entrez] AID - 10.1186/s12938-026-01610-w [pii] AID - 10.1186/s12938-026-01610-w [doi] PST - epublish SO - Biomed Eng Online. 2026 Aug 2;25(1):122. doi: 10.1186/s12938-026-01610-w. PMID- 42958355 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2050-7283 (Electronic) IS - 2050-7283 (Linking) VI - 14 IP - 1 DP - 2026 Aug 4 TI - Validation of the Multidimensional Scale of Perceived Social Support (MSPSS) in Dine bizaad (Navajo): randomized comparison of a community translation versus a forward translation. LID - 1569 [pii] LID - 10.1186/s40359-026-05285-9 [doi] AB - BACKGROUND: The Multidimensional Scale of Perceived Social Support (MSPSS) is a 12-item psychometric instrument that assesses perceived social support (PSS) with a well-established three-factor structure: family, friends, and significant others. Having a way to measure PSS is important because PSS is only moderately correlated to objective social support. As one of the most widely used and cited measures of PSS, the MSPSS has been translated from English into dozens of languages. However, some authors have been critical of some MSPSS translation and validation studies, particularly when the translation processes do not conduct confirmatory factor analyses or report different numbers of factors when validating the translated instrument. Previously, members of our research team conducted a community-engaged study to develop a culturally-informed translation of the MSPSS into Dine bizaad (Navajo). This study aimed to examine the factor structure and internal reliability of the community-based translation of the MSPSS and a control condition (a basic forward translation) and to provide a descriptive comparison of the results by study arm. METHODS: We conducted a randomized controlled trial with 1:1 blinded allocation to compare the community-engaged translation of the MSPSS to a control condition. Two-hundred bilingual Navajo/English speakers in La Plata County, CO were randomly assigned to complete one of the two instruments (n = 100 per study arm). We conducted confirmatory factor analysis on each arm separately, using a robust MLR estimator with listwise deletion to compute both absolute and incremental fit indices, and computed Cronbach's alpha for reliability of each subscale. RESULTS: Both translations adequately fit the three-factor structure. While we did not conduct direct statistical comparisons, descriptive comparisons indicated that the forward translation showed better model fit in terms of incremental indices as well as one of the absolute fit indices (SRMR), while the community translation showed two better absolute fit indices (RMSEA and chi(2)/df ratio). Subscale internal consistency ranged from .72 to .78 for the community translation and from .87 to .89 for the forward translation. CONCLUSIONS: Contrary to our expectations, the fit indices were mixed and did not clearly support one particular translation. We discuss potential reasons for this finding and implications thereof. CI - (c) 2026. The Author(s). FAU - Dutta, Tapati AU - Dutta T AD - Public Health Department, School of Arts and Sciences, Fort Lewis College, Durango, CO, USA. AD - Department of Applied Health Science, Indiana University School of Public Health-Bloomington, Bloomington, IN, USA. FAU - Begay, Tse'yi'nii I I AU - Begay TII AD - Chemistry Department, School of Arts and Sciences, Fort Lewis College, Durango, CO, USA. FAU - Williams, Jaddah AU - Williams J AD - Public Health Department, School of Arts and Sciences, Fort Lewis College, Durango, CO, USA. FAU - Zimet, Gregory AU - Zimet G AD - Department of Pediatrics (Emeritus), Indiana University School of Medicine, Indianapolis, IN, USA. AD - Zimet Research Consulting, Indianapolis, IN, USA. FAU - Radtke, Johanna AU - Radtke J AD - Biostatistics Consulting Center, Indiana University School of Public Health-Bloomington, Bloomington, IN, USA. FAU - Dickinson, Stephanie AU - Dickinson S AD - Biostatistics Consulting Center, Indiana University School of Public Health-Bloomington, Bloomington, IN, USA. FAU - Agley, Jon AU - Agley J AD - Department of Applied Health Science, Indiana University School of Public Health-Bloomington, Bloomington, IN, USA. jagley@iu.edu. AD - Prevention Insights, Indiana University School of Public Health-Bloomington, Bloomington, IN, USA. jagley@iu.edu. LA - eng PT - Comparative Study PT - Journal Article PT - Randomized Controlled Trial PT - Validation Study DEP - 20260804 PL - England TA - BMC Psychol JT - BMC psychology JID - 101627676 SB - IM MH - Humans MH - Reproducibility of Results MH - *Psychometrics MH - *Social Support MH - Female MH - Male MH - Adult MH - Factor Analysis, Statistical MH - Surveys and Questionnaires/standards MH - Translations MH - Young Adult MH - Translating OTO - NOTNLM OT - Community engaged research OT - Dine OT - Factor analysis OT - MSPSS OT - Mental health OT - Multidimensional Scale of Perceived Social Support OT - Navajo OT - Randomized controlled trial OT - Social support OT - Validation COIS- Declarations. Ethics approval and consent to participate: This study was reviewed by the Fort Lewis College IRB prior to any data collection and received a designation of "Exempt" (IRB-2025-165), and thereafter the study complied with all relevant regulations and guidelines. As approved by that IRB, all participants provided informed consent in the form of "implied consent," which means that participants were provided with study information in written or verbal form, depending on their preference, and verbally indicated their consent to participate. Also, as approved by the Fort Lewis College IRB, the study information did not include the detail that the survey form was randomly selected from one of two translations. Consent for publication: Not applicable. Competing interests: Unrelated to this manuscript, GZ wishes to disclose that he has served as an external advisory board member for Pfizer, Moderna, Merck, and Sanofi, and as a consultant to Merck and Pfizer; has received investigator-initiated research funding from Merck administered through Indiana University; and serves as an unpaid member of the Board of Directors for the Unity Consortium, a nonprofit organization that supports adolescent and young adult health through vaccination. None of the other authors reported any competing interests related to the content of this manuscript. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:02 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/06/03 00:00 [received] PHST- 2026/07/31 00:00 [accepted] PHST- 2026/10/30 01:02 [entrez] AID - 10.1186/s40359-026-05285-9 [pii] AID - 10.1186/s40359-026-05285-9 [doi] PST - epublish SO - BMC Psychol. 2026 Aug 4;14(1):1569. doi: 10.1186/s40359-026-05285-9. PMID- 42958353 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 1475-2867 (Print) IS - 1475-2867 (Linking) VI - 26 IP - 1 DP - 2026 Oct 27 TI - Indole analogs and the aryl sulfonamide AqB011 potently restrain the invasiveness of glioblastoma cells, as determined in ex vivo xenograft tissue slice co-cultures (XTCCs). LID - 353 [pii] LID - 10.1186/s12935-026-04489-8 [doi] AB - BACKGROUND: Glioblastomas (GBMs) are highly invasive and heterogeneous in nature which strongly contributes to poor survival of patients, averaging 15 months after diagnosis. With little treatment options being available in particular for addressing GBM invasiveness, there is a high need for supplementary therapies in addition to classical cytostatics like Temozolomide (TMZ). METHODS: The aim of this study was to examine promising compounds, the natural indole scaffold RAD114 and three semisynthetic analogs (RAD118, RAD119, RAD120) as well as the aryl sulfonamide AqB011, for their inhibitory potential specifically on tumor cell invasion. For this, GBM xenograft tissue slice tandem co-culture (XTCC) models were employed, closely reflecting the in vivo patient situation. In particular, these ex vivo tissue slice air-liquid interface (ALI) models also allow for distinguishing between inhibitory effects on bulk tumor growth vs tumor cell invasion into the normal brain tissue. RESULTS: Profound anti-invasive effects of the natural indole scaffold RAD114, three semisynthetic analogs (RAD118, RAD119, RAD120), and the aryl sulfonamide AqB011 were observed in XTCC models based on G55T2 and U87-MG GBM xenografts co-cultured with mouse brain slices in ALI tandem co-culture. While effects on bulk tumor growth were rather moderate, profound inhibition or even abolishment of tumor invasion was observed, dependent on the compound, its dosage and the model. At a non-toxic concentration (10 microM), RAD118 and AqB011 showed the highest effectiveness in reducing tumor invasion. Combination effects at sub-optimal compound dosages were observed as well. CONCLUSIONS: Both compound classes - the tested semisynthetic indole analogs and the sulfonamide derivative - may be promising lead candidates for drugs useful to specifically address the issue of GBM invasiveness. They strongly restrained GBM cell invasion, alone and in combination. Rather than acting through cytotoxic effects, they specifically inhibit cell migration into native brain tissue. CI - (c) 2026. The Author(s). FAU - Wagner, Kim K AU - Wagner KK AD - School of Pharmacy and Biomedical Science, College of Health, Adelaide University, Adelaide, 5005, Australia. AD - Rudolf-Boehm-Institute for Pharmacology and Toxicology, Medical Faculty, University of Leipzig, Hartelstrasse 16-18, Leipzig, D-04107, Germany. AD - Rudolf-Boehm-Institute for Pharmacology and Toxicology, Clinical Pharmacology, Medical Faculty, University of Leipzig, Hartelstrasse 16-18, Leipzig, D-04107, Germany. FAU - Yool, Andrea J AU - Yool AJ AD - School of Pharmacy and Biomedical Science, College of Health, Adelaide University, Adelaide, 5005, Australia. FAU - Davis, Rohan A AU - Davis RA AD - Institute for Biomedicine and Glycomics, Griffith University, Nathan, 4111, QLD, Australia. AD - School of Environment and Science, Griffith University, Nathan, 4111, QLD, Australia. AD - NatureBank, Griffith University, Nathan, 4111, QLD, Australia. FAU - Becker, Katrin AU - Becker K AD - Rudolf-Boehm-Institute for Pharmacology and Toxicology, Medical Faculty, University of Leipzig, Hartelstrasse 16-18, Leipzig, D-04107, Germany. FAU - Franke, Heike AU - Franke H AD - Rudolf-Boehm-Institute for Pharmacology and Toxicology, Medical Faculty, University of Leipzig, Hartelstrasse 16-18, Leipzig, D-04107, Germany. heike.franke@medizin.uni-leipzig.de. FAU - Aigner, Achim AU - Aigner A AUID- ORCID: 0000-0002-2778-6256 AD - Rudolf-Boehm-Institute for Pharmacology and Toxicology, Clinical Pharmacology, Medical Faculty, University of Leipzig, Hartelstrasse 16-18, Leipzig, D-04107, Germany. achim.aigner@medizin.uni-leipzig.de. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Cancer Cell Int JT - Cancer cell international JID - 101139795 OTO - NOTNLM OT - Aquaporin OT - Glioblastoma OT - Indolamide OT - Invasion OT - Semisynthetic indole analogs OT - Tissue slice co-culture models COIS- Declarations. Ethics approval and consent to participate: All mouse studies were approved by the responsible research ethics committee and the local authorities (TVV 59/20 and TVV23/24, Saxony Regional Directorate) and carried out according to the national regulations of animal welfare and the national legislation on the use of animals for research (Animal Welfare Act, Animal Welfare Experimental Animal Ordinance). They complied with the ARRIVE guidelines and the EU Directive 2010/63/EU for animal experiments, including strict and careful consideration of the 3R principles, measures to reduce animal suffering, and the definition of humane endpoints and euthanasia methods. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:02 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/03 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/30 01:02 [entrez] AID - 10.1186/s12935-026-04489-8 [pii] AID - 10.1186/s12935-026-04489-8 [doi] PST - epublish SO - Cancer Cell Int. 2026 Oct 27;26(1):353. doi: 10.1186/s12935-026-04489-8. PMID- 42958352 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 1753-2000 (Print) IS - 1753-2000 (Linking) VI - 20 IP - 1 DP - 2026 Jul 27 TI - Risk prediction models for non-suicidal self-injury in adolescents with depression: a systematic review and meta-analysis. LID - 149 [pii] LID - 10.1186/s13034-026-01137-x [doi] AB - BACKGROUND: Non-suicidal self-injury (NSSI) is prevalent among adolescents with depression and is a significant predictor of suicidal behavior. Although several risk prediction models for NSSI have been developed, their overall performance, methodological quality, and feasibility for clinical translation remain unclear. OBJECTIVE: This study aimed to comprehensively evaluate the performance of NSSI risk prediction models for adolescents with depression through a systematic review and meta-analysis, summarize their area under the curve (AUC) values, and determine the incidence of NSSI and associated predictors in this population. METHODS: PubMed, Web of Science, The Cochrane Library, CINAHL, Embase, Scopus, PsycINFO, CNKI, and Wanfang Database were searched from inception to September 20, 2025. The GRADE approach, PROBAST, and TRIPOD tools were used to assess the overall certainty of evidence, risk of bias, and reporting quality, respectively. Data were extracted using the CHARMS checklist. The PROBAST and TRIPOD tools were used to assess the risk of bias and reporting quality, respectively. Meta-analyses of model AUC values and NSSI incidence were performed using Stata 17.0. RESULTS: Eighteen studies (comprising 40 models) were included. The meta-analysis revealed a pooled NSSI incidence of 50% (95% CI = 0.29-0.71) among adolescents with depression. The models demonstrated promising discriminative ability: the pooled AUC was 0.82 (95% CI = 0.79-0.86) for development cohorts, 0.90 (95% CI = 0.87-0.93) for internal validation cohorts, and 0.82 (95% CI = 0.77-0.86) for external validation cohorts. The most frequently identified predictors included depression severity, childhood trauma, sex, emotional abuse, and emotional neglect. However, the PROBAST assessment revealed a high risk of bias in most studies, particularly in the domains of participant selection and statistical analysis. The TRIPOD assessment indicated insufficient reporting of key methodological details such as sample size justification and handling of missing data. Only five studies conducted external validation. The certainty of evidence assessed by GRADE was low to very low for the main outcomes, mainly because of high risk of bias, substantial heterogeneity, indirectness, and limited validation evidence. CONCLUSION: Existing prediction models show promising discriminative potential for identifying NSSI risk in adolescents with depression. However, high AUC values should be interpreted cautiously because methodological bias, substantial heterogeneity, limited external validation, incomplete reporting, and population differences may have led to overly optimistic performance estimates. Future research should focus on building more robust, trustworthy, and clinically translatable tools through prospective designs, adequate sample sizes, standardized analytical methods, extensive external validation, and complete reporting adhering to the TRIPOD guidelines. CI - (c) 2026. The Author(s). FAU - Xie, Xuemei AU - Xie X AD - Sichuan Provincial Center for Mental Health, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, No. 33, Section 2, Furong Avenue, Wenjiang District 611137, Chengdu, China. FAU - Li, Yue AU - Li Y AD - West China Second Hospital of Sichuan University, Chengdu, China. FAU - Li, Hang AU - Li H AD - Sichuan Provincial Center for Mental Health, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, No. 33, Section 2, Furong Avenue, Wenjiang District 611137, Chengdu, China. FAU - Gan, Tiantian AU - Gan T AD - Sichuan Provincial Center for Mental Health, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, No. 33, Section 2, Furong Avenue, Wenjiang District 611137, Chengdu, China. FAU - Huang, Linyu AU - Huang L AD - Sichuan Provincial Center for Mental Health, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, No. 33, Section 2, Furong Avenue, Wenjiang District 611137, Chengdu, China. FAU - Cheng, Jia AU - Cheng J AD - Sichuan Provincial Center for Mental Health, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, No. 33, Section 2, Furong Avenue, Wenjiang District 611137, Chengdu, China. 314881087@qq.com. LA - eng PT - Journal Article PT - Review DEP - 20260727 PL - England TA - Child Adolesc Psychiatry Ment Health JT - Child and adolescent psychiatry and mental health JID - 101297974 OTO - NOTNLM OT - Adolescents OT - Depression OT - NSSI OT - Prediction model OT - Systematic review COIS- Declarations. Ethics approval and consent to participate: As this was a review with meta-analysis, approval from an ethics committee was not required. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:02 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/12/11 00:00 [received] PHST- 2026/07/01 00:00 [accepted] PHST- 2026/10/30 01:02 [entrez] AID - 10.1186/s13034-026-01137-x [pii] AID - 10.1186/s13034-026-01137-x [doi] PST - epublish SO - Child Adolesc Psychiatry Ment Health. 2026 Jul 27;20(1):149. doi: 10.1186/s13034-026-01137-x. PMID- 42958349 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1475-2875 (Electronic) IS - 1475-2875 (Linking) VI - 25 IP - 1 DP - 2026 Aug 1 TI - High prevalence of pfmdr1 and pfpm2 amplification and pfmdr1 haplotypes in Plasmodium falciparum isolates circulating in Ghana. LID - 385 [pii] LID - 10.1186/s12936-026-06075-9 [doi] AB - BACKGROUND: In Ghana, artemether-lumefantrine (AL), and artesunate-amodiaquine (AS-AQ) are the main first-line ACTs for uncomplicated malaria, with dihydroartemisinin-piperaquine (DHA-PPQ) as an alternative treatment regimen. Copy Number Polymorphisms (CNPs) in Plasmodium falciparum multidrug resistance 1 (pfmdr1) and plasmepsin 2 (pfpm2) genes, as well as specific pfmdr1 haplotypes have been implicated in resistance to AL and DHA-PPQ partner drugs. METHODS: This study characterised pfmdr1 and pfpm2 CNPs and pfmdr1 haplotypes in 900 clinical malaria samples across three transmission zones in Ghana. Using a SYBR Green-based Quantitative real-time Polymerase Chain Reaction (qPCR) assay, followed by targeted amplicon sequencing. RESULTS: Successful pfmdr1 and pfpm2 amplifications were detected in 32.0% (n = 288) and 27.8% (n = 250) isolates respectively. No significant difference in the prevalence of these gene amplifications was observed across the three eco-epidemiological zones (pfmdr1: p = 0.888; pfpm2: p = 0.081). Furthermore, a weak positive correlation was observed between pfmdr1 and pfpm2 copy numbers (rho = 0.196), suggesting limited co-selection of these loci. Pfmdr1 haplotype analysis at codons N86Y, Y184F, and D1246Y revealed a high prevalence (55.6%) of the NFD haplotype linked to lumefantrine selection. Interestingly, this haplotype allele was equally prevalent among isolates carrying single or multiple gene copy numbers. CONCLUSION: Taken together, this study observed increased prevalence of pfmdr1 and pfpm2 gene amplification in Ghana. Also, a high frequency of the single mutant NFD pfmdr1 haplotype suggesting ongoing selection pressure potentially driven by widespread AL use in Ghana. In addition, pfpm2 CNP has been associated with piperaquine resistance. These findings provide a critical baseline for ongoing molecular surveillance as an early warning for ACT partner drug resistance in Ghana. CI - (c) 2026. The Author(s). FAU - Kassim, Abdul Rashid AU - Kassim AR AD - West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana. FAU - Younge, Priscilla Akuba AU - Younge PA AD - West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana. FAU - Dorvi, Ignatus Nunana AU - Dorvi IN AD - West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana. FAU - Saiid, Samirah AU - Saiid S AD - Department of Biology, University of Virginia, Charlottesville, VA, USA. FAU - Sakyi, Mona-Liza Ebiere AU - Sakyi ME AD - West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana. FAU - Ahmed, Osumanu AU - Ahmed O AD - West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana. FAU - Kudakpo, Albert Yao AU - Kudakpo AY AD - West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana. FAU - Addo, Stephen Tetteh AU - Addo ST AD - West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana. FAU - Yeboah, Silas Nkansah AU - Yeboah SN AD - West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana. FAU - Mensah, Charles AU - Mensah C AD - West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana. FAU - Debrah, Isaiah AU - Debrah I AD - West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana. FAU - Morang'a, Collins Misita AU - Morang'a CM AD - West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana. FAU - Amuzu, Dominic S Y AU - Amuzu DSY AD - West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana. FAU - Awandare, Gordon A AU - Awandare GA AD - West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana. AD - Department of Biochemistry, Cell and Molecular Biology, College of Basic and Applied Sciences, University of Ghana, Accra, Ghana. FAU - Aniweh, Yaw AU - Aniweh Y AD - West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana. FAU - Amenga-Etego, Lucas Naam-Kayagre AU - Amenga-Etego LN AD - West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Accra, Ghana. lamengaetego@ug.edu.gh. LA - eng GR - 134717/National Institute for Health and Care Research/ PT - Journal Article DEP - 20260801 PL - England TA - Malar J JT - Malaria journal JID - 101139802 RN - 0 (ATP-Binding Cassette, Sub-Family C Proteins) RN - 0 (Mdr1 protein, Plasmodium falciparum) RN - 0 (Protozoan Proteins) RN - 0 (Antimalarials) RN - EC 3.4.23.- (Aspartic Acid Endopeptidases) RN - EC 3.4.23.39 (plasmepsin II) RN - 0 (Quinolines) SB - IM MH - *ATP-Binding Cassette, Sub-Family C Proteins/genetics/metabolism MH - Ghana/epidemiology MH - *Plasmodium falciparum/genetics/isolation & purification MH - *Haplotypes MH - *Protozoan Proteins/genetics/metabolism MH - Antimalarials/pharmacology MH - *Aspartic Acid Endopeptidases/genetics MH - Humans MH - *Malaria, Falciparum/parasitology/epidemiology MH - *Gene Amplification MH - Prevalence MH - DNA Copy Number Variations MH - Real-Time Polymerase Chain Reaction MH - Quinolines/pharmacology OTO - NOTNLM OT - Plasmodium falciparum OT - Copy number polymorphism OT - Haplotypes OT - Multidrug resistance OT - Plasmepsin COIS- Declarations. Ethics approval and consent to participate: Written informed consent was obtained from all adult participants and from the parents or guardians of minors. Ethical approval for sample collection and subsequent analysis was granted by the Ghana Health Service Ethics Review Committee (GHS-ERC:007/08/24). Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:02 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/19 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/30 01:02 [entrez] AID - 10.1186/s12936-026-06075-9 [pii] AID - 10.1186/s12936-026-06075-9 [doi] PST - epublish SO - Malar J. 2026 Aug 1;25(1):385. doi: 10.1186/s12936-026-06075-9. PMID- 42958347 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1868-7083 (Electronic) IS - 1868-7075 (Linking) VI - 18 IP - 1 DP - 2026 Aug 1 TI - DNA methylation of silencer-annotated regions at the MGMT locus in the context of promoter and enhancer methylation in glioblastoma and melanoma brain metastases. LID - 198 [pii] LID - 10.1186/s13148-026-02204-y [doi] AB - BACKGROUND: MGMT promoter methylation is a well-established biomarker in glioblastoma, its relevance in melanoma brain metastases remains controversial. Recent studies indicate that methylation of MGMT enhancers contributes to MGMT regulation, whereas the role of silencer methylation has not yet been investigated. METHODS: DNA methylation of 52 CpG sites was analyzed in regions annotated as potential silencers located in the promoter, intronic, and upstream regions of MGMT. These regions were selected based on genomic annotations and their genomic proximity to MGMT, encompassing sites to approximately 1.5 Mb upstream, without experimental validation of direct regulatory activity. Analyses were performed in glioblastoma and melanoma brain metastases using in-house developed assays based on bisulfite conversion, PCR amplification of target regions, and pyrosequencing. By integrating previously published promoter and enhancer methylation data, a DNA methylation landscape of MGMT regulatory elements was established. Associations between CpG methylation and MGMT protein expression, overall survival, patient age, and sex were evaluated. RESULTS: The degree of methylation strongly depended on the genomic location of the respective regulatory element. Both shared and tumor-type-specific methylation patterns were observed in glioblastoma and melanoma brain metastases. Promoter-associated CpGs and selected intronic silencer-annotated regions showed associations with MGMT protein expression. In glioblastoma, several promoter-associated CpGs were associated with overall survival, whereas sex-specific and age-related effects were absent. CONCLUSIONS: DNA methylation of MGMT regulatory elements is highly CpG-specific. CpG-resolved analyses reveal a complex epigenetic landscape across the MGMT locus and highlight substantial regional heterogeneity. These findings suggest that extending methylation analyses beyond the classical promoter region provides additional insights into MGMT-associated epigenetic variation and its potentical clinical associations. CI - (c) 2026. The Author(s). FAU - Puhringer, Katharina AU - Puhringer K AD - Institute of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria. AD - Vienna Doctoral School in Chemistry (DoSChem), University of Vienna, 1090, Vienna, Austria. FAU - Firlinger, Fabian AU - Firlinger F AD - Institute of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria. FAU - Fehringer, Benno AU - Fehringer B AD - Institute of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria. FAU - Weis, Serge AU - Weis S AD - Division of Neuropathology, Department of Pathology and Molecular Pathology, Kepler University Hospital GmbH, Johannes Kepler University, Linz, Austria. AD - Clinical Research Institute for Neurosciences, Johannes Kepler University, Linz, Austria. FAU - Spiegl-Kreinecker, Sabine AU - Spiegl-Kreinecker S AD - Department of Neurosurgery, Johannes Kepler University, Kepler University Hospital GmbH, Linz, Austria. AD - Clinical Research Institute for Neurosciences, Johannes Kepler University, Linz, Austria. FAU - Cichna-Markl, Margit AU - Cichna-Markl M AUID- ORCID: 0000-0001-8699-674X AD - Institute of Analytical Chemistry, Faculty of Chemistry, University of Vienna, Vienna, Austria. margit.cichna@univie.ac.at. LA - eng PT - Journal Article DEP - 20260801 PL - Germany TA - Clin Epigenetics JT - Clinical epigenetics JID - 101516977 RN - EC 6.5.1.- (DNA Repair Enzymes) RN - EC 2.1.1.- (DNA Modification Methylases) RN - EC 2.1.1.63 (MGMT protein, human) RN - 0 (Tumor Suppressor Proteins) SB - IM MH - Humans MH - *DNA Methylation MH - *DNA Repair Enzymes/genetics MH - *DNA Modification Methylases/genetics MH - *Brain Neoplasms/genetics/secondary MH - Promoter Regions, Genetic MH - *Melanoma/genetics/pathology/secondary MH - *Tumor Suppressor Proteins/genetics MH - *Glioblastoma/genetics MH - Female MH - CpG Islands MH - Male MH - Enhancer Elements, Genetic MH - Middle Aged MH - Aged MH - Gene Expression Regulation, Neoplastic MH - Epigenesis, Genetic MH - Adult COIS- Declarations. Ethics approval and consent to participate: The study was conducted in accordance with the Declaration of Helsinki and approved by the Ethics Commission (application number E-39-15). Competing interests: The authors declare no competing interests. Consent for publication: Not applicable. Informed consent: Informed consent was obtained from all subjects involved in the study. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:01 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/01 00:00 [received] PHST- 2026/07/12 00:00 [accepted] PHST- 2026/10/30 01:01 [entrez] AID - 10.1186/s13148-026-02204-y [pii] AID - 10.1186/s13148-026-02204-y [doi] PST - epublish SO - Clin Epigenetics. 2026 Aug 1;18(1):198. doi: 10.1186/s13148-026-02204-y. PMID- 42958344 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1756-3305 (Electronic) IS - 1756-3305 (Linking) VI - 19 IP - 1 DP - 2026 Aug 3 TI - The hidden malaria: misidentification of Plasmodium malariae and Plasmodium ovale in low-transmission setting targeted for elimination in Ethiopia. LID - 464 [pii] LID - 10.1186/s13071-026-07511-0 [doi] AB - BACKGROUND: Quartan malaria caused by Plasmodium malariae (Pm) and tertian Plasmodium ovale (Po) malaria has been largely overlooked in Ethiopia due to their milder clinical symptoms, lower prevalence, and frequently misdiagnosis during routine microscopic. This study estimated the prevalence of Pm and Po infections misdiagnosed as P. vivax (Pv) in selected health facilities in southwestern Ethiopia. METHODS: A cross-sectional study was conducted among study participants who were positive for Pv by microscopy or rapid diagnostic test (RDT) and who were attending health facilities within Arjo-Didessa sugarcane plantations and surrounding areas in Oromia, southwestern Ethiopia, between September 2019 and July 2022. Of the 3590 febrile patients attending health facilities, 323 were Pv positive by microscopy or RDT. Among these, 245 Pv-positive samples were re-evaluated using quantitative polymerase chain reaction (qPCR). Species-specific primers targeting Pv, P. falciparum (Pf), Pm, and Po were used for species confirmation. RESULTS: From 245 vivax malaria cases, RDT were performed to 162 sub-samples, among them 8.6% (14/162) were negative by RDT. None of the samples tested by RDT and microscopy detected Pm or Po. Of these 245 tested and reported as Pv positive by microscopy, qPCR confirmed that 215 (87.7%, 95% CI 82.98-91.58) were positive for Pv, 9 (3.7%, 95% CI 1.69-6.86) for Pf, 8 (3.3%, 95% CI 1.42-6.33) for Pm, 6 (2.4%, 95% CI 0.90-5.25) were positive for Po, and 7 (2.8%, 95% CI 1.16-5.80) had mixed infections (Pf and Pv). CONCLUSIONS: The findings of this study highlights the need for a strategic shift to integrate Pm and Po into Ethiopia's malaria control and elimination program. Moreover, strengthening the diagnostic capacity should include training of microscopists for accurate Plasmodium species identification and adopting highly sensitive diagnostic tool for all four Plasmodium species in resource-limited settings. This is essential for enhancing malaria control and accelerating progress toward elimination. CI - (c) 2026. The Author(s). FAU - Getachew, Hallelujah AU - Getachew H AD - School of Medical Laboratory Sciences, Institute of Health, Jimma University, Jimma, Ethiopia. hgetachew4@gmail.com. AD - Department of Medical Laboratory Sciences, Arba Minch College of Health Sciences, Arba Minch, Ethiopia. hgetachew4@gmail.com. AD - Tropical and Infectious Diseases Research Center (TIDRC), Jimma University, Jimma, Ethiopia. hgetachew4@gmail.com. FAU - Wang, Chloe AU - Wang C AD - Department of Population Health and Disease Prevention, University of California at Irvine, Irvine, CA, 92697, USA. FAU - Habtamu, Kassahun AU - Habtamu K AD - Menelik II Medical & Health Science College, Addis Ababa, Ethiopia. FAU - Demissew, Assalif AU - Demissew A AD - Department of Medical Laboratory Sciences, College of Medicine and Health Sciences, Ambo University, Ambo, Ethiopia. FAU - Degefa, Teshome AU - Degefa T AD - School of Medical Laboratory Sciences, Institute of Health, Jimma University, Jimma, Ethiopia. AD - Tropical and Infectious Diseases Research Center (TIDRC), Jimma University, Jimma, Ethiopia. FAU - Birhanie, Solomon K AU - Birhanie SK AD - West Valley Mosquito and Vector Control Agency, Ontario, CA, USA. FAU - Kazura, James W AU - Kazura JW AD - Center for Global Health and Diseases, Case Western Reserve University, Cleveland, OH, USA. FAU - King, Christopher L AU - King CL AD - Center for Global Health and Diseases, Case Western Reserve University, Cleveland, OH, USA. AD - Veterans Affairs Medical Center, Cleveland, OH, USA. FAU - Yan, Guiyun AU - Yan G AD - Department of Population Health and Disease Prevention, University of California at Irvine, Irvine, CA, 92697, USA. FAU - Yewhalaw, Delenasaw AU - Yewhalaw D AD - School of Medical Laboratory Sciences, Institute of Health, Jimma University, Jimma, Ethiopia. AD - Tropical and Infectious Diseases Research Center (TIDRC), Jimma University, Jimma, Ethiopia. LA - eng GR - D43 TW001505/The US National Institutes of Health/ GR - R01 A1050243/The US National Institutes of Health/ GR - U19 AI129326/The US National Institutes of Health/ PT - Journal Article DEP - 20260803 PL - England TA - Parasit Vectors JT - Parasites & vectors JID - 101462774 SB - IM MH - Ethiopia/epidemiology MH - Humans MH - *Plasmodium ovale/isolation & purification/genetics MH - *Plasmodium malariae/isolation & purification/genetics MH - Cross-Sectional Studies MH - *Malaria/epidemiology/diagnosis/parasitology/transmission MH - Rapid Diagnostic Tests MH - Microscopy MH - Prevalence MH - *Diagnostic Errors/statistics & numerical data MH - Female MH - Male MH - Plasmodium vivax/isolation & purification/genetics MH - Adult OTO - NOTNLM OT - Plasmodium malariae OT - Plasmodium ovale OT - Ethiopia OT - Species misidentification OT - qPCR COIS- Declarations. Ethics approval and consent to participate: The National Research Ethics Review Committee (NRERC) (Ref. No. 3.10/131/2018) and Jimma University Institute of Health Institutional Review Board (IRB) granted ethical approval (Ref. No IHRPJn/479). The local health authorities also gave permission to conduct the study in the selected health facilities, specifically the Jimma-Arjo District Health Office (Ref. No. 0178/JA/2019) and the Dabo-Hanna District Health Office (Ref. No. WF/662/19). Following a thorough description of the study's goals and protocols, with the right to discontinue participation at any moment without consequence, each participant gave written informed consent. Parents or legal guardians signed the informed consent form for all minors. In addition, minors between the ages of 12 and 17 were requested to give their own assent. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:01 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/15 00:00 [received] PHST- 2026/06/04 00:00 [accepted] PHST- 2026/10/30 01:01 [entrez] AID - 10.1186/s13071-026-07511-0 [pii] AID - 10.1186/s13071-026-07511-0 [doi] PST - epublish SO - Parasit Vectors. 2026 Aug 3;19(1):464. doi: 10.1186/s13071-026-07511-0. PMID- 42958343 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1477-7819 (Electronic) IS - 1477-7819 (Linking) VI - 24 IP - 1 DP - 2026 Aug 1 TI - Clinicopathological features, recurrence patterns, and survival outcomes of triple-negative ductal carcinoma in situ: a single-center cohort study. LID - 410 [pii] LID - 10.1186/s12957-026-04505-1 [doi] AB - BACKGROUND: Triple-negative ductal carcinoma in situ (TN-DCIS) is uncommon, and its prognostic significance remains unclear. We aimed to characterize the clinicopathological features of TN-DCIS and evaluate its association with recurrence outcomes. METHODS: We retrospectively reviewed patients with pathologically confirmed pure DCIS treated at Tianjin Central Hospital of Gynecology Obstetrics between January 2008 and December 2021. Patients with microinvasive or invasive carcinoma were excluded. Patients were classified as TN-DCIS or non-TN DCIS according to immunohistochemistry. The primary endpoint was disease-free survival (DFS). Secondary endpoints included ipsilateral breast tumor recurrence (IBTR)-free survival, invasive recurrence-free survival, and time to invasive recurrence. Survival outcomes were estimated using Kaplan-Meier methods and compared with log-rank tests. Cox regression was used to explore factors associated with DFS. RESULTS: Among 111 patients, 13 (11.7%) had TN-DCIS and 98 had non-TN DCIS. Within 5 years of follow-up, DFS events occurred in four patients with TN-DCIS and one patient with non-TN DCIS. The corresponding 5-year DFS rate was lower in the TN-DCIS group than in the non-TN DCIS group (69.23% [95% CI, 48.19-99.47] vs. 98.98% [95% CI, 97.01-100.00]; p < 0.001). In exploratory multivariable Cox analysis, TN-DCIS was associated with poorer DFS (HR 5.990, 95% CI, 1.686-21.280; p = 0.0056). Breastfeeding history was associated with lower recurrence risk (HR 0.123, 95% CI, 0.030-0.513; p = 0.004), whereas CK5/6 positivity was associated with increased recurrence risk (HR 5.525, 95% CI, 1.302-23.451; p = 0.0205). TN-DCIS was also associated with poorer 5-year IBTR-free survival and invasive recurrence-free survival, with similar exploratory findings observed in the breast-conserving surgery subgroup. CONCLUSIONS: TN-DCIS was uncommon but associated with increased recurrence risk. The triple-negative phenotype may identify a clinically higher-risk DCIS subgroup. These findings warrant validation in larger multicenter cohorts and future evaluation of tailored surveillance strategies. CI - (c) 2026. The Author(s). FAU - Liu, Jingwei AU - Liu J AD - The Second Surgical Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, 300060, China. AD - Tianjin's Clinical Research Center for Cancer, Tianjin, 300060, China. AD - Key Laboratory of Breast Cancer Prevention and Therapy, Ministry of Education, Tianjin Medical University, Tianjin, 300060, China. AD - Department of Breast Surgery, Tianjin Central Hospital of Gynecology Obstetrics, Tianjin, China. FAU - Zhang, Nan AU - Zhang N AD - Department of Liver Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100730, China. FAU - Yu, Xiujie AU - Yu X AD - Department of Pathology, Tianjin Central Hospital of Gynecology Obstetrics, Tianjin, China. FAU - Feng, Rui AU - Feng R AD - Department of Breast Surgery, Tianjin Central Hospital of Gynecology Obstetrics, Tianjin, China. dr.fr@126.com. FAU - Liu, Hong AU - Liu H AD - The Second Surgical Department of Breast Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjin, 300060, China. liuhong_submit@tjmuch.com. AD - Tianjin's Clinical Research Center for Cancer, Tianjin, 300060, China. liuhong_submit@tjmuch.com. AD - Key Laboratory of Breast Cancer Prevention and Therapy, Ministry of Education, Tianjin Medical University, Tianjin, 300060, China. liuhong_submit@tjmuch.com. LA - eng PT - Journal Article DEP - 20260801 PL - England TA - World J Surg Oncol JT - World journal of surgical oncology JID - 101170544 SB - IM MH - Humans MH - Female MH - *Neoplasm Recurrence, Local/pathology/epidemiology/mortality MH - Retrospective Studies MH - *Carcinoma, Intraductal, Noninfiltrating/pathology/mortality/surgery MH - *Triple Negative Breast Neoplasms/pathology/mortality/surgery MH - Middle Aged MH - Prognosis MH - Survival Rate MH - Follow-Up Studies MH - Adult MH - Aged MH - Disease-Free Survival OTO - NOTNLM OT - Breast-conserving surgery OT - Disease-free survival OT - Ductal carcinoma in situ OT - Invasive recurrence OT - Ipsilateral breast tumor recurrence OT - Triple-negative breast cancer COIS- Declarations. Ethics approval and consent to participate: The study protocol followed the Declaration of Helsinki, was approved by the Institutional Review Board of Tianjin Central Hospital of Gynecology Obstetrics. Written informed consent was waived. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:01 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/08 00:00 [received] PHST- 2026/07/16 00:00 [accepted] PHST- 2026/10/30 01:01 [entrez] AID - 10.1186/s12957-026-04505-1 [pii] AID - 10.1186/s12957-026-04505-1 [doi] PST - epublish SO - World J Surg Oncol. 2026 Aug 1;24(1):410. doi: 10.1186/s12957-026-04505-1. PMID- 42958340 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 2050-2974 (Print) IS - 2050-2974 (Linking) VI - 14 IP - 1 DP - 2026 Aug 3 TI - Psychometric properties of the Farsi version of the Night Eating Disorder Questionnaire. LID - 255 [pii] LID - 10.1186/s40337-026-01726-3 [doi] AB - OBJECTIVE: This study assesses the factor structure and psychometric properties of Farsi version of Night Eating Questionnaire. METHODS: In this cross-sectional study, we randomly selected 155 people referred to Shafa hospital and private psychiatry clinic in Rasht City, Iran. After translation to Farsi and back-translation to English, the face and content validity of NEQ Farsi version were evaluated using the opinions of a panel of experts and calculating the content validity ratio and content validity index. Then, the construct validity was evaluated by Confirmatory Factor Analysis (CFA), the Cronbach alpha coefficient was used for assessing internal consistency and the Intra class Correlation Coefficient (ICC) was used for assessing test-retest reliability. RESULTS: The mean age of participants was 41.1 +/- 12.9 Years. The CFA results showed that the four-factor model had a good fit. Cronbach's alpha showed an overall coefficient of 0.72. Spearman's correlation coefficient was 0.97 and the intra-class correlation coefficient was 0.99 (95% CI 0.97-0.99), indicating a satisfactory level of test-retest reliability. CONCLUSIONS: The Farsi version of the NEQ has acceptable psychometric properties for the Farsi language and Iranian culture, to be used as a valid and reliable tool in future research, care, and therapeutic activities. CI - (c) 2026. The Author(s). FAU - Khalkhali, Mohammadrasoul AU - Khalkhali M AUID- ORCID: 0000-0002-8824-4629 AD - Department of Psychiatry, Kavosh Cognitive Behavior Sciences and Addiction Research Center, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran. FAU - Fatemi, Saghar AU - Fatemi S AUID- ORCID: 0000-0001-6189-5803 AD - Department of Psychiatry, Kavosh Cognitive Behavior Sciences and Addiction Research Center, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran. saghar_10@hotmail.com. FAU - Yazdanipour, Mohammadali AU - Yazdanipour M AUID- ORCID: 0000-0001-9084-0887 AD - Neuroscience Research Center, Trauma Institute, Guilan University of Medical Sciences, Rasht, Iran. FAU - Khalkhali, Parnian AU - Khalkhali P AD - School of Medicine, Guilan University of Medical Sciences, Rasht, Iran. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - J Eat Disord JT - Journal of eating disorders JID - 101610672 OTO - NOTNLM OT - Eating disorder OT - Night Eating Questionnaire(NEQ) OT - Psychometrics OT - Surveys and questionnaires COIS- Declarations. Ethics approval and consent to particpate: This study was approved by the Ethics Committee of Guilan University of Medical Sciences, Rasht, Iran (Code: IR.GUMS.REC. 1403. 522) The participants in the study were informed about the purpose and method of the study, and they were assured that the information collected would remain confidential. Finally, after obtaining their written consent and providing necessary explanations regarding how to fill out the questionnaire, the data was collected. They were free to leave the study at any time. Informed consent was obtained from all participants. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:01 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/01/06 00:00 [received] PHST- 2026/07/24 00:00 [accepted] PHST- 2026/10/30 01:01 [entrez] AID - 10.1186/s40337-026-01726-3 [pii] AID - 10.1186/s40337-026-01726-3 [doi] PST - epublish SO - J Eat Disord. 2026 Aug 3;14(1):255. doi: 10.1186/s40337-026-01726-3. PMID- 42958339 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 2052-1847 (Print) IS - 2052-1847 (Linking) VI - 18 IP - 1 DP - 2026 Aug 1 TI - Development of the football tactical skills scale and comparison of scores across player and training characteristics. LID - 532 [pii] LID - 10.1186/s13102-026-01934-z [doi] AB - BACKGROUND: Tactical performance in football emerges from the interaction of perceptual-cognitive processes, decision-making, positional responsibilities, psychological regulation, and coordinated action under changing game constraints. Although tactical behaviour can be examined through observational and game-based procedures, there remains a need for a standardized, practically administered self-report instrument that captures how players evaluate their own tactical actions across different developmental and competitive contexts. This study aimed to develop and initially validate the Football Tactical Skills Scale (FTSS) and to examine whether FTSS scores differed according to selected player, training, and psychosocial characteristics. METHODS: The study used a cross-sectional survey and scale-development design. The scale-development process included a pilot study (n = 50), exploratory factor analysis (n = 320), and confirmatory factor analysis (n = 320). Group comparisons were conducted in a sample of 700 football players aged 14 years and older (x̄(age)=18.22 +/- 4.60). Content validity, construct validity, internal consistency, lower-upper group discrimination, and convergent and discriminant validity were examined. Descriptive statistics, one-way analysis of variance, independent-samples t-tests, Tamhane's T2 post-hoc tests, eta-squared, and Cohen's d were used in the comparative analyses. RESULTS: The 17-item FTSS comprised four dimensions: game reading and decision-making, psychological skills and game discipline, transitions in game and field utilization, and strategic thinking and risk management. The factor structure showed acceptable-to-excellent fit and satisfactory reliability and validity indicators. Higher self-reported FTSS scores were observed among older players, players competing at higher league levels, players reporting more weekly training sessions, mental training, visual support training, and better communication with coaches. Scores also differed by playing position; midfielders obtained the highest total scores, whereas wingers obtained the highest scores in strategic thinking and risk management (p<0.001). CONCLUSIONS: The findings provide initial support for the FTSS as a multidimensional self-report questionnaire for assessing players' perceived tactical skills in football. The scale was sensitive to systematic differences across age, position, competitive level, training exposure, and selected psychosocial characteristics. Because the study was cross-sectional and did not include an external criterion of observed tactical performance, FTSS scores should not be interpreted as direct measures of match behaviour or as evidence of causal effects. Future research should examine test-retest reliability, measurement invariance, cross-cultural validity, and criterion validity against tools such as FUT-SAT, GPAI, coach ratings, notational analysis, and representative small-sided games. CI - (c) 2026. The Author(s). FAU - Eroglu, Oguzhan AU - Eroglu O AUID- ORCID: 0000-0002-0409-2745 AD - Sports Management Department, Mus Alparslan University Varto Vocational School, Mus, 49000, Turkey. o.eroglu@alparslan.edu.tr. FAU - Karadag, Alper AU - Karadag A AUID- ORCID: 0000-0002-8931-2523 AD - Faculty of Sports Sciences, Mus Alparslan University, Mus, Turkey. LA - eng PT - Journal Article DEP - 20260801 PL - England TA - BMC Sports Sci Med Rehabil JT - BMC sports science, medicine & rehabilitation JID - 101605016 OTO - NOTNLM OT - Decision-making OT - Football OT - Scale development OT - Self-report OT - Tactical skills COIS- Declarations. Ethics approval and consent to participate: The entire protocol was approved by the Mus Alparslan University Ethics Commission (February 10, 2025-182180), and written informed consent was obtained from all participants or their legal representatives before inclusion in the study. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. Author Note: This article was derived from the doctoral dissertation of Oguzhan Eroglu. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:01 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/20 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/30 01:01 [entrez] AID - 10.1186/s13102-026-01934-z [pii] AID - 10.1186/s13102-026-01934-z [doi] PST - epublish SO - BMC Sports Sci Med Rehabil. 2026 Aug 1;18(1):532. doi: 10.1186/s13102-026-01934-z. PMID- 42958337 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1476-511X (Electronic) IS - 1476-511X (Linking) VI - 25 IP - 1 DP - 2026 Aug 3 TI - Sex-dimorphic phenotypic divergence in metabolically driven thyroid nodules: the interplay of systemic inflammation and adiposity. LID - 216 [pii] LID - 10.1186/s12944-026-03021-1 [doi] AB - BACKGROUND: Metabolic dysfunction has been increasingly implicated in thyroid nodule development; however, the sex-specific interplay between systemic glucolipotoxicity, adiposity, inflammation, and thyroid nodule risk remains poorly understood. This study aimed to evaluate the association between the composite cholesterol, high-density lipoprotein, and glucose (CHG) index and thyroid nodules (TNs) and to explore the potential factors underlying sex-specific differences across Thyroid Imaging Reporting and Data System (TI-RADS) risk categories. METHODS: This large-scale health check-up cohort included 24,878 participants who underwent routine health examinations in a unique sub-plateau cohort (altitude, ~ 2,000 m). The TNs were identified using high-resolution ultrasonography and classified using the TI-RADS grading system. The CHG index was used to quantify systemic metabolic burden. Guided by directed acyclic graphs (DAGs) for covariate selection, multivariable logistic regression was used to evaluate independent associations, and restricted cubic splines were used to assess nonlinear dose-response relationships. Statistical mediation analysis using red blood cell distribution width (RDW) as the mediator quantified the proportion attributable to systemic inflammation. RESULTS: Multivariable analyses revealed significant sex-dimorphic associations between the CHG index and TN risk. In men, elevated CHG levels were consistently associated with increased risk across nodular grades. Notably, the association with high-risk nodules (TI-RADS 4) was markedly attenuated and no longer statistically significant after adjusting for BMI, suggesting that adiposity may partly account for this association. Mediation analysis indicated that low-grade systemic inflammation (RDW) statistically explained a significant proportion of the metabolic-nodule association in men. In women, higher CHG was inversely associated with early-stage (TI-RADS 2) nodules. This pattern was more apparent among women aged >/= 50 years, suggesting potential age-related heterogeneity; however, this finding should be interpreted with caution because direct hormonal data were unavailable. Furthermore, the CHG index demonstrated superior risk reclassification utility compared to isolated lipid parameters. CONCLUSION: The CHG index is associated with thyroid risk stratification in a sex-specific manner. These findings indicate that sex-dependent physiological characteristics may modify the association between metabolic factors and high-risk thyroid morphological patterns. In men, these patterns were more strongly associated with adiposity and inflammation, whereas in older women, these patterns may be related to adaptive morphological changes. These findings may inform sex-specific metabolic management strategies for thyroid care. CI - (c) 2026. The Author(s). FAU - Fan, Huanqiong AU - Fan H AD - Department of Pharmacy, Foshan University Affiliated Third Hospital, Guangdong, China. FAU - Yin, Yingyong AU - Yin Y AD - Department of Clinical Nutrition, Northeast Yunnan Central Hospital, Northeast Yunnan Hospital of Kunming Medical University, Northeast Yunnan Clinical School of Kunming Medical University, Zhaotong, 657000, Yunnan, China. FAU - Huang, Shaopeng AU - Huang S AD - Department of Endocrinology, Foshan University Affiliated Third Hospital, Guangdong, China. FAU - Cui, Zhibo AU - Cui Z AD - Information Technology Department, Northeast Yunnan Central HospitalNortheast Yunnan Hospital of Kunming Medical UniversityNortheast Yunnan Clinical School of Kunming Medical University, Zhaotong, 657000, Yunnan, China. FAU - Dao, Ling AU - Dao L AD - Department of Medical Ultrasonics, Northeast Yunnan Central HospitalNortheast Yunnan Hospital of Kunming Medical UniversityNortheast Yunnan Clinical School of Kunming Medical University, Zhaotong, 657000, Yunnan, China. FAU - Li, Daishan AU - Li D AD - Nursing Department, Northeast Yunnan Central HospitalNortheast Yunnan Hospital of Kunming Medical UniversityNortheast Yunnan Clinical School of Kunming Medical University, Zhaotong, 657000, Yunnan, China. FAU - Qu, Xiaoxiao AU - Qu X AD - Department of Clinical Laboratory, The Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang, China. FAU - Jiang, Guosong AU - Jiang G AUID- ORCID: 0009-0009-2367-4079 AD - Department of Pulmonary and Critical Care Medicine, Northeast Yunnan Central HospitalNortheast Yunnan Hospital of Kunming Medical UniversityNortheast Yunnan Clinical School of Kunming Medical University, Zhaotong, 657000, Yunnan, China. jiangguosong@kmmu.edu.cn. AD - Kunming Medical University, Kunming, 650500, Yunnan, China. jiangguosong@kmmu.edu.cn. FAU - Qiu, Chi-E AU - Qiu CE AUID- ORCID: 0009-0008-4423-2766 AD - Hematology Department, Northeast Yunnan Central HospitalNortheast Yunnan Hospital of Kunming Medical UniversityNortheast Yunnan Clinical School of Kunming Medical University, Zhaotong, 657000, Yunnan, China. 13508702069@139.com. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - Lipids Health Dis JT - Lipids in health and disease JID - 101147696 RN - 97C5T2UQ7J (Cholesterol) RN - 0 (Blood Glucose) RN - 0 (Lipoproteins, HDL) SB - IM MH - Humans MH - Male MH - Female MH - *Adiposity MH - *Thyroid Nodule/metabolism/pathology/blood/diagnostic imaging MH - *Inflammation/blood/metabolism MH - Middle Aged MH - Sex Characteristics MH - Phenotype MH - Risk Factors MH - Adult MH - Cholesterol/blood MH - Blood Glucose/metabolism MH - Sex Factors MH - Lipoproteins, HDL/blood OTO - NOTNLM OT - Cholesterol High-density lipoprotein and glucose index OT - Inflammation OT - Metabolic syndrome OT - Sex dimorphism OT - Thyroid nodules COIS- Declarations. Ethics approval and consent to participate: Approval of the research protocol: This study was approved by the Ethics Committee of Northeast Yunnan Central Hospital (Approval No. kj2026012) and conducted in strict accordance with the principles of the Declaration of Helsinki. Informed Consent: The requirement for written informed consent was waived by the Ethics Committee of Northeast Yunnan Central Hospital owing to the retrospective nature of the study and exclusive use of de-identified clinical data. Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:01 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/06 00:00 [received] PHST- 2026/07/25 00:00 [accepted] PHST- 2026/10/30 01:01 [entrez] AID - 10.1186/s12944-026-03021-1 [pii] AID - 10.1186/s12944-026-03021-1 [doi] PST - epublish SO - Lipids Health Dis. 2026 Aug 3;25(1):216. doi: 10.1186/s12944-026-03021-1. PMID- 42958331 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1475-2891 (Electronic) IS - 1475-2891 (Linking) VI - 25 IP - 1 DP - 2026 Aug 1 TI - Effects of selenium supplementation on glycemic indices and oxidative-stress biomarkers in adults: a systematic review, meta-analysis, and dose-response analysis of randomized controlled trials. LID - 118 [pii] LID - 10.1186/s12937-026-01376-w [doi] AB - BACKGROUND: Diabetes and metabolic syndrome are major public health challenges closely linked to oxidative stress, inflammation, and impaired insulin signaling. Selenium, an essential trace element with antioxidant and immunomodulatory roles, has been proposed as an adjunctive strategy for metabolic regulation; however, evidence from randomized trials remains inconsistent. We evaluated the effects of selenium supplementation on glycemic indices, oxidative-stress-related biomarkers, lipid metabolism, and related metabolic indicators in adults, and explored potential effect modifiers. METHODS: We searched PubMed, Embase, Cochrane CENTRAL, Web of Science, CINAHL, CNKI, WanFang, VIP, and SinoMed through 12 August 2025 for randomized controlled trials of oral selenium supplementation in adults, including pregnant women with gestational diabetes mellitus (GDM). Outcomes included fasting plasma glucose, glycated hemoglobin, fasting insulin, indices of insulin resistance and beta-cell function, oxidative-stress-related and inflammatory biomarkers, lipid parameters, and anthropometric and hemodynamic indicators. Random-effects meta-analyses estimated weighted mean differences with 95% confidence intervals. Subgroup, dose-response, and sensitivity analyses were performed, and certainty of evidence was assessed using GRADE. RESULTS: Fifty-two treatment arms involving 10,916 participants were included. Selenium supplementation reduced fasting insulin, HOMA-IR, and HOMA-beta values and increased QUICKI, but showed no clear overall effect on fasting plasma glucose or glycated hemoglobin. Favorable changes were observed in selected antioxidant and inflammatory biomarkers, including increases in glutathione, total antioxidant capacity, superoxide dismutase, glutathione peroxidase, and serum selenium, and reductions in malondialdehyde and high-sensitivity C-reactive protein, with no clear effect on nitric oxide. Modest changes in lipid parameters were also observed, including higher high-density lipoprotein cholesterol and lower low-density lipoprotein cholesterol, triglycerides, and total cholesterol. Among anthropometric and hemodynamic outcomes, only diastolic blood pressure was reduced. Effects varied by dose, duration, participant characteristics, and geographical selenium exposure context. CONCLUSIONS: Selenium supplementation was associated with modest improvements in insulin-sensitivity markers, selected antioxidantand inflammatory biomarkers, and lipid parameters, while showing no clear overall effect on fasting plasma glucose or glycated hemoglobin. These findings may be most applicable to populations with marginal or low baseline selenium status and should be extrapolated cautiously to selenium-replete settings. Long-term, high-quality RCTs are needed to clarify baseline-status-specific effects, safe dose ranges, and long-term safety. TRIAL REGISTRATION: PROSPERO CRD420251124852. CI - (c) 2026. The Author(s). FAU - Yang, Xin-Yue AU - Yang XY AD - Center for Evidence-Based Medicine and Clinical Research, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, People's Republic of China. FAU - Xu, Pan AU - Xu P AD - Department of Critical Care Medicine, Zhongnan Hospital of Wuhan University, Wuhan, 430071, People's Republic of China. FAU - Tan, Xin AU - Tan X AD - Center for Evidence-Based Medicine and Clinical Research, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, People's Republic of China. FAU - Sun, Yu-Ting AU - Sun YT AD - Department of Pathology, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, People's Republic of China. FAU - Tang, Min AU - Tang M AD - Department of Clinical Nutrition, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, People's Republic of China. FAU - Zhang, Chao AU - Zhang C AD - Center for Evidence-Based Medicine and Clinical Research, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, People's Republic of China. FAU - Wang, Long AU - Wang L AD - Center for Evidence-Based Medicine and Clinical Research, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, People's Republic of China. ashtonwang@qq.com. FAU - Wu, Shi-Shi AU - Wu SS AD - Center for Evidence-Based Medicine and Clinical Research, Taihe Hospital, Hubei University of Medicine, Shiyan, 442000, People's Republic of China. sunshine.ashton@qq.com. AD - Department of Histology and Embryology, School of Basic Medical Sciences, Hubei University of Medicine, Shiyan, 442000, People's Republic of China. sunshine.ashton@qq.com. LA - eng GR - X202110929025/Innovation and Entrepreneurship Training Program/ GR - 24Y064/Shiyan City Scientific Research Project/ PT - Journal Article PT - Meta-Analysis PT - Review PT - Systematic Review DEP - 20260801 PL - England TA - Nutr J JT - Nutrition journal JID - 101152213 RN - H6241UJ22B (Selenium) RN - 0 (Biomarkers) RN - 0 (Blood Glucose) RN - 0 (Antioxidants) RN - 0 (Insulin) RN - 0 (Glycated Hemoglobin) SB - IM MH - Humans MH - *Selenium/administration & dosage/pharmacology MH - *Oxidative Stress/drug effects MH - Biomarkers/blood MH - *Dietary Supplements MH - Randomized Controlled Trials as Topic MH - Female MH - Adult MH - Blood Glucose/drug effects MH - *Glycemic Index/drug effects MH - Antioxidants/pharmacology/administration & dosage MH - Pregnancy MH - Insulin Resistance MH - Dose-Response Relationship, Drug MH - Insulin/blood MH - Glycated Hemoglobin OTO - NOTNLM OT - Antioxidant biomarkers OT - Glycemic control OT - Insulin resistance OT - Lipid profile OT - Meta-analysis OT - Oxidative stress OT - Selenium supplementation COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:01 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/04 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/30 01:01 [entrez] AID - 10.1186/s12937-026-01376-w [pii] AID - 10.1186/s12937-026-01376-w [doi] PST - epublish SO - Nutr J. 2026 Aug 1;25(1):118. doi: 10.1186/s12937-026-01376-w. PMID- 42958328 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1477-3155 (Electronic) IS - 1477-3155 (Linking) VI - 24 IP - 1 DP - 2026 Aug 1 TI - A platinum-osmium hybrid nanozyme potentiates chemo-radio-immunotherapy for colorectal cancer via tumor microenvironment remodeling and cGAS-STING activation. LID - 1007 [pii] LID - 10.1186/s12951-026-04883-4 [doi] AB - BACKGROUND: Concurrent systemic chemotherapy and local radiotherapy represent the clinical standard of care for a broad spectrum of malignant tumors, and these modalities can elicit endogenous anti-tumor immune responses. However, the hypoxic tumor microenvironment (TME) of solid tumors severely impairs the therapeutic efficacy of chemotherapy, radiotherapy, and immunotherapy, leading to treatment failure and tumor progression. RESULTS: We engineered a platinum-osmium hybrid nanozyme (PO) via the self-assembly of an oxaliplatin (Oxa) prodrug, osmium, and polyvinylpyrrolidone, which potentiates the synergy of combined therapy through cascade catalytic reactions. PO exhibits catalase-like activity to decompose endogenous hydrogen peroxide into oxygen, thereby reversing tumor hypoxia, and glutathione peroxidase-like activity to deplete intracellular glutathione, which synergistically augments the efficacy of chemotherapy and radiotherapy. Under the reductive TME, PO is activated to release bioactive Oxa, in conjunction with osmium-mediated radiosensitization, PO induces severe DNA damage, activates the cyclic GMP-AMP synthase-stimulator of interferon genes pathway, and triggers robust immunogenic cell death, thus eliciting a systemic anti-tumor immune response. Furthermore, PO modulates the expression of metastasis-associated epithelial-mesenchymal transition proteins to inhibit tumor invasion and metastasis. Both in vitro and in vivo studies demonstrate that PO exerts superior therapeutic efficacy against metastatic colorectal cancer compared with free Oxa. Notably, the biodegradable nature of PO minimizes systemic toxicity and ensures favorable biocompatibility. CONCLUSIONS: This work presents a degradable hybrid nanozyme strategy that integrates chemotherapeutic, radiosensitizing, and immunomodulatory functions to overcome the hypoxic TME barrier, thus improving the therapeutic outcome of hypoxic and metastatic colorectal cancer. CI - (c) 2026. The Author(s). FAU - Fu, Nannan AU - Fu N AD - Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, NMPA Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou, 511436, P. R. China. FAU - Guo, Liyou AU - Guo L AD - Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, NMPA Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou, 511436, P. R. China. FAU - Zhan, Zhixiong AU - Zhan Z AD - Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, NMPA Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou, 511436, P. R. China. FAU - Chen, Zeer AU - Chen Z AD - Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, NMPA Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou, 511436, P. R. China. FAU - Chen, Jiaxuan AU - Chen J AD - Guangdong Province Key Laboratory of Pharmaceutical Bioactive Substances, School of Bioscience and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, P. R. China. FAU - Li, Qiuhua AU - Li Q AD - Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, NMPA Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou, 511436, P. R. China. FAU - Li, Jingyao AU - Li J AD - Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, NMPA Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou, 511436, P. R. China. FAU - He, Gen AU - He G AD - Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, NMPA Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou, 511436, P. R. China. hegen@gzhmu.edu.cn. FAU - Zheng, Yue AU - Zheng Y AD - Guangdong Province Key Laboratory of Pharmaceutical Bioactive Substances, School of Bioscience and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, P. R. China. zhengyue@gdpu.edu.cn. FAU - Zhang, Dong-Yang AU - Zhang DY AUID- ORCID: 0000-0003-3940-8911 AD - Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target & Clinical Pharmacology, NMPA Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou, 511436, P. R. China. zhangdy7@gzhmu.edu.cn. LA - eng GR - 22207019/National Natural Science Foundation of China/ PT - Journal Article DEP - 20260801 PL - England TA - J Nanobiotechnology JT - Journal of nanobiotechnology JID - 101152208 RN - 49DFR088MY (Platinum) RN - 0 (Prodrugs) RN - 04ZR38536J (Oxaliplatin) RN - 0 (Antineoplastic Agents) RN - EC 2.7.7.86 (Cyclic Guanosine Monophosphate-Adenosine Monophosphate Synthase) RN - 0 (Radiation-Sensitizing Agents) SB - IM MH - Animals MH - *Tumor Microenvironment/drug effects MH - *Colorectal Neoplasms/drug therapy/therapy/metabolism/pathology MH - Humans MH - *Platinum/chemistry/pharmacology MH - Immunotherapy/methods MH - cGAS-STING Signaling Pathway MH - Mice MH - Cell Line, Tumor MH - Prodrugs/pharmacology/chemistry MH - Oxaliplatin/pharmacology/chemistry MH - Antineoplastic Agents/pharmacology/chemistry MH - Cyclic Guanosine Monophosphate-Adenosine Monophosphate Synthase MH - Mice, Inbred BALB C MH - Radiation-Sensitizing Agents/pharmacology MH - Female OTO - NOTNLM OT - Epithelial-mesenchymal transition OT - Immunotherapy OT - Nanozyme OT - Platinum(IV) prodrug OT - Radiosensitization OT - Tumor microenvironment COIS- Declarations. Ethics approval and consent to participate: All animal experiments were approved by the Animal Ethics Committee of Guangzhou Medical University (Approval No.: GY 2025 - 292). Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:00 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/14 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/30 01:00 [entrez] AID - 10.1186/s12951-026-04883-4 [pii] AID - 10.1186/s12951-026-04883-4 [doi] PST - epublish SO - J Nanobiotechnology. 2026 Aug 1;24(1):1007. doi: 10.1186/s12951-026-04883-4. PMID- 42958326 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1755-8794 (Electronic) IS - 1755-8794 (Linking) VI - 19 IP - 1 DP - 2026 Jul 31 TI - Haplotype-based non-invasive prenatal testing of F8 complex rearrangement via targeted capture sequencing of maternal plasma. LID - 163 [pii] LID - 10.1186/s12920-026-02442-2 [doi] AB - BACKGROUND: Hemophilia A (HA) is an X-linked recessive bleeding disorder primarily caused by the intron 22 inversion (Inv22) in F8 gene, which accounts for approximately 45% of all cases. A minor proportion of HA results from complex structural rearrangements (CSRs) in F8 gene. However, the ability of non-invasive prenatal testing (NIPT) to detect F8-related CSRs has not been evaluated. METHODS: In this study, we applied NIPT to evaluate the risk of HA in a male fetus, whose mother had a history of delivering a boy affected by severe HA. Optical genome mapping (OGM) had previously identified the complex structural rearrangement (CSR) in F8 gene as the definitive genetic etiology of the first child's severe HA, and was confirmed to be maternally inherited. High-throughput sequencing was performed on the trios (the first child and both parents). Parental haplotypes were deduced through haplotype linkage analysis based on single nucleotide polymorphisms (SNPs). Subsequently, cell-free DNA in maternal plasma was subjected to sequencing, and fetal haplotypes were successfully inferred using the Hidden Markov Model (HMM) with parental haplotype information. RESULTS: NIPT identified a male fetus who had inherited the CSR in the F8 gene from his mother, suggesting a diagnosis of HA. The NIPT findings were subsequently verified by OGM analysis of chorionic villi. CONCLUSION: Our study demonstrates that haplotype-based NIPT is an effective approach for detecting CSRs in the F8 gene during prenatal screening. CI - (c) 2026. The Author(s). FAU - Zhang, Yuxin AU - Zhang Y AD - The Central Laboratory of Birth Defects Prevention and Control, The Affiliated Women and Children's Hospital of Ningbo University, 339 Liuting Street, Haishu District, Ningbo, Zhejiang, 315012, China. AD - Ningbo Key Laboratory for the Prevention and Treatment of Embryogenic Diseases, The Affiliated Women and Children's Hospital of Ningbo University, 339 Liuting Street, Haishu District, Ningbo, Zhejiang, 315012, China. AD - Ningbo Key Laboratory of Genomic Medicine and Birth Defects Prevention, The Affiliated Women and Children's Hospital of Ningbo University, 339 Liuting Street, Haishu District, Ningbo, Zhejiang, 315012, China. FAU - Xue, Jiangyang AU - Xue J AD - The Central Laboratory of Birth Defects Prevention and Control, The Affiliated Women and Children's Hospital of Ningbo University, 339 Liuting Street, Haishu District, Ningbo, Zhejiang, 315012, China. AD - Ningbo Key Laboratory for the Prevention and Treatment of Embryogenic Diseases, The Affiliated Women and Children's Hospital of Ningbo University, 339 Liuting Street, Haishu District, Ningbo, Zhejiang, 315012, China. AD - Ningbo Key Laboratory of Genomic Medicine and Birth Defects Prevention, The Affiliated Women and Children's Hospital of Ningbo University, 339 Liuting Street, Haishu District, Ningbo, Zhejiang, 315012, China. FAU - Zhuang, Danyan AU - Zhuang D AD - The Central Laboratory of Birth Defects Prevention and Control, The Affiliated Women and Children's Hospital of Ningbo University, 339 Liuting Street, Haishu District, Ningbo, Zhejiang, 315012, China. AD - Ningbo Key Laboratory for the Prevention and Treatment of Embryogenic Diseases, The Affiliated Women and Children's Hospital of Ningbo University, 339 Liuting Street, Haishu District, Ningbo, Zhejiang, 315012, China. AD - Ningbo Key Laboratory of Genomic Medicine and Birth Defects Prevention, The Affiliated Women and Children's Hospital of Ningbo University, 339 Liuting Street, Haishu District, Ningbo, Zhejiang, 315012, China. FAU - Pan, Xiaoli AU - Pan X AD - The Central Laboratory of Birth Defects Prevention and Control, The Affiliated Women and Children's Hospital of Ningbo University, 339 Liuting Street, Haishu District, Ningbo, Zhejiang, 315012, China. AD - Ningbo Key Laboratory for the Prevention and Treatment of Embryogenic Diseases, The Affiliated Women and Children's Hospital of Ningbo University, 339 Liuting Street, Haishu District, Ningbo, Zhejiang, 315012, China. AD - Ningbo Key Laboratory of Genomic Medicine and Birth Defects Prevention, The Affiliated Women and Children's Hospital of Ningbo University, 339 Liuting Street, Haishu District, Ningbo, Zhejiang, 315012, China. FAU - Wu, Yiming AU - Wu Y AD - Shanghai WeHealth BioMedical Technology Co., Ltd., Shanghai, China. FAU - Yang, Jingmin AU - Yang J AD - Shanghai WeHealth BioMedical Technology Co., Ltd., Shanghai, China. FAU - Chen, Changshui AU - Chen C AD - Ningbo Key Laboratory for the Prevention and Treatment of Embryogenic Diseases, The Affiliated Women and Children's Hospital of Ningbo University, 339 Liuting Street, Haishu District, Ningbo, Zhejiang, 315012, China. FAU - Wu, Shanshan AU - Wu S AD - Ningbo Key Laboratory of Genomic Medicine and Birth Defects Prevention, The Affiliated Women and Children's Hospital of Ningbo University, 339 Liuting Street, Haishu District, Ningbo, Zhejiang, 315012, China. FAU - Li, Haibo AU - Li H AUID- ORCID: 0000-0002-9309-6632 AD - The Central Laboratory of Birth Defects Prevention and Control, The Affiliated Women and Children's Hospital of Ningbo University, 339 Liuting Street, Haishu District, Ningbo, Zhejiang, 315012, China. lihaibo-775@163.com. AD - Ningbo Key Laboratory for the Prevention and Treatment of Embryogenic Diseases, The Affiliated Women and Children's Hospital of Ningbo University, 339 Liuting Street, Haishu District, Ningbo, Zhejiang, 315012, China. lihaibo-775@163.com. AD - Ningbo Key Laboratory of Genomic Medicine and Birth Defects Prevention, The Affiliated Women and Children's Hospital of Ningbo University, 339 Liuting Street, Haishu District, Ningbo, Zhejiang, 315012, China. lihaibo-775@163.com. LA - eng GR - No.2025KY1426/the Medical and Health Science and Technology Program of Zhejiang Province/ GR - No. 2026-A34/Ningbo Leading Medical & Health Discipline/ GR - No.2023Z178/the Ningbo Science and Technology Project/ GR - No.2025Z160/the Key Technology Breakthrough Program of Ningbo Sci-Tech Innovation YONGJIANG 2035/ GR - No.2024Z221/the Key Technology Breakthrough Program of Ningbo Sci-Tech Innovation YONGJIANG 2035/ PT - Case Reports PT - Journal Article DEP - 20260731 PL - England TA - BMC Med Genomics JT - BMC medical genomics JID - 101319628 RN - 9001-27-8 (Factor VIII) SB - IM MH - Humans MH - *Haplotypes MH - Female MH - Pregnancy MH - Male MH - *Factor VIII/genetics MH - Polymorphism, Single Nucleotide MH - *Hemophilia A/genetics/diagnosis/blood MH - *Noninvasive Prenatal Testing/methods MH - *Gene Rearrangement MH - High-Throughput Nucleotide Sequencing MH - *Prenatal Diagnosis/methods OTO - NOTNLM OT - Complex structural rearrangements OT - Haplotype linkage analysis OT - Non-invasive prenatal testing COIS- Declarations. Ethics approval and consent to participate: In accordance with the Declaration of Helsinki, The pregnant woman provided informed consent to participate in the study. This study was approved by the IRB of The Affiliated Women and Children's Hospital of Ningbo University (No. EC2024-184). Consent for publication: The pregnant woman has signed the written informed consent, agreeing that her and her family members' personal or clinical information, as well as any identifiable images, may be used for the publication of this study. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:00 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/06 00:00 [received] PHST- 2026/07/24 00:00 [accepted] PHST- 2026/10/30 01:00 [entrez] AID - 10.1186/s12920-026-02442-2 [pii] AID - 10.1186/s12920-026-02442-2 [doi] PST - epublish SO - BMC Med Genomics. 2026 Jul 31;19(1):163. doi: 10.1186/s12920-026-02442-2. PMID- 42958325 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 1752-4458 (Print) IS - 1752-4458 (Linking) VI - 20 IP - 1 DP - 2026 Aug 2 TI - Psychiatric education in medical training: a scoping review of curricular innovations, teaching strategies, and assessment methods. LID - 40 [pii] LID - 10.1186/s13033-026-00724-x [doi] AB - BACKGROUND: Mental disorders contribute substantially to global disease burden, yet persistent deficiencies remain in medical trainees' readiness to identify, diagnose, and treat patients with mental health conditions. Main barriers include stigma, outdated curricula, insufficient clinical practice, and regional differences in mental health services. OBJECTIVES: This scoping review systematically mapped existing research on educational innovations, learning approaches, and evaluation instruments in psychiatric education for medical learners (2015-2026), with formal quality appraisal using a JBI-adapted tool. METHODS: Following JBI methodology and PRISMA-ScR guidelines, a systematic search was conducted across MEDLINE, Embase, PsycINFO, and Scopus. The PCC model guided eligibility. Two reviewers independently selected articles and extracted data. A ten-item JBI-adapted quality appraisal checklist was applied to all included studies. The evidence base comprised 34 primary empirical studies (77.3%) and 10 secondary or contextual sources (22.7%). RESULTS: Of 3,682 articles identified, 44 met inclusion criteria 34 primary empirical studies (77.3%) and 10 secondary sources (22.7%). Most originated from the United States (54.5%), focusing on undergraduate learners (88.6%). Quality appraisal revealed only 34.1% used validated measures, 20.5% included follow-up, and 18.2% fully met control group criteria. Thematic analysis identified five themes: curricular innovations, educational approaches, assessment methods, Kirkpatrick-classified outcomes, and implementation barriers/facilitators. While all 44 reported Level 1 and Level 2 outcomes, measured in primary studies and discussed in secondary sources, no studies measured Level 3 behavioral change or Level 4 patient outcomes. CONCLUSIONS: The absence of Level 3 and Level 4 evidence represents a major limitation and priority for future research; it does not imply health systems cannot invest in psychiatric education, but that the evidence base has not yet demonstrated translation to improved clinical behaviors or patient outcomes. Future research should prioritize longitudinal and system-level evaluation with validated behavioral and patient-level outcome measures. CI - (c) 2026. The Author(s). FAU - Aqtam, Ibrahim AU - Aqtam I AUID- ORCID: 0000-0001-6339-9176 AD - Department of Nursing, Ibn Sina College for Health Professions, Nablus University for Vocational and Technical Education, Nablus, 00970, Palestine. ibrahim.aqtam@nu-vte.edu.ps. FAU - Mansor, Khulud AU - Mansor K AUID- ORCID: 0009-0005-1870-2988 AD - Department of Nursing, Ibn Sina College for Health Professions, Nablus University for Vocational and Technical Education, Nablus, 00970, Palestine. FAU - Shouli, Mustafa AU - Shouli M AUID- ORCID: 0009-0006-0560-9327 AD - Department of Nursing, Ibn Sina College for Health Professions, Nablus University for Vocational and Technical Education, Nablus, 00970, Palestine. LA - eng PT - Journal Article DEP - 20260802 PL - England TA - Int J Ment Health Syst JT - International journal of mental health systems JID - 101294224 OTO - NOTNLM OT - Ccurricular innovation OT - JBI quality appraisal OT - Kirkpatrick evaluation OT - Medical education OT - Psychiatric education OT - Scoping review OT - Simulation-based education OT - Stigma reduction COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 01:00 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/29 00:00 [received] PHST- 2026/07/17 00:00 [accepted] PHST- 2026/10/30 01:00 [entrez] AID - 10.1186/s13033-026-00724-x [pii] AID - 10.1186/s13033-026-00724-x [doi] PST - epublish SO - Int J Ment Health Syst. 2026 Aug 2;20(1):40. doi: 10.1186/s13033-026-00724-x. PMID- 42958313 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1478-6362 (Electronic) IS - 1478-6354 (Linking) VI - 28 IP - 1 DP - 2026 Aug 4 TI - Integrative proteomic and machine learning strategies identify key biomarkers associated with response to JAK inhibitor in rheumatoid arthritis. LID - 191 [pii] LID - 10.1186/s13075-026-03880-0 [doi] AB - INTRODUCTION: Janus kinase inhibitors (JAKi) have revolutionized the treatment of rheumatoid arthritis (RA), yet therapeutic responses vary widely across patients. Identifying proteomic biomarkers of response may enhance personalized treatment strategies and improve clinical outcomes. MATERIALS AND METHODS: We conducted a prospective study involving 42 RA patients treated with tofacitinib, baricitinib, or upadacitinib. Longitudinal serum proteomic profiling was performed at baseline and 6 months using the Olink(R) Explore 384 platform. Treatment response was defined by ACR/EULAR criteria. Biomarkers associated with good responders were identified through differential expression analysis and ensemble feature selection. Prediction models were developed using selected features, and feature importance was quantified using SHapley Additive exPlanations (SHAP). RESULTS: Pretreatment levels of IL-12B, IL-17A, and IFN-gamma were significantly elevated in JAKi non-responders. IL-17 signaling was associated with response to baricitinib and tofacitinib, while upadacitinib responders exhibited broader suppression of immune-related proteins including IL-4/IL-13 pathways. Prediction models using proteomic features outperformed those based on clinical variables alone (AUC >/= 0.90 vs. 0.52 - 0.57), with further improvement upon integrating clinical and proteomic data (AUC = 0.95). Among all features, IL-12B emerged as the most robust and consistent predictor of treatment response, supported by both differential expression and multiple machine learning approaches. CONCLUSION: Serum proteomic profiling identifies elevated pretreatment IL-12B levels as a key predictor of JAKi non-response and uncovers distinct molecular responses to different JAK inhibitors. These findings support the utility of integrating proteomics with machine learning to advance personalized treatment strategies in RA. CI - (c) 2026. The Author(s). FAU - Chen, Shuo-Fu AU - Chen SF AD - Department of Heavy Particles & Radiation Oncology, Taipei Veterans General Hospital, Taipei, Taiwan. FAU - Wu, Ting-Shuan AU - Wu TS AD - Department of Biomedical Sciences, Chung Shan Medical University, Taichung, Taiwan. AD - Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan. AD - Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan. FAU - Chang, Kuang-Yi AU - Chang KY AD - Department of Biomedical Sciences and Engineering, Institute of Systems Biology and Bioinformatics, National Central University, Taoyuan, Taiwan. FAU - Lo, Sheng-Min AU - Lo SM AD - Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan. FAU - Chen, Yi-Ting AU - Chen YT AD - Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan. FAU - Kao, Chung-Mao AU - Kao CM AD - Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan. AD - Division of Allergy, Immunology and Rheumatology, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan. FAU - Chen, Yen-Ju AU - Chen YJ AD - Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan. AD - Division of Allergy, Immunology and Rheumatology, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan. AD - School of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan. FAU - Chang, Hui-Yin AU - Chang HY AD - Department of Biomedical Sciences and Engineering, Institute of Systems Biology and Bioinformatics, National Central University, Taoyuan, Taiwan. dr.chuiyin@gmail.com. FAU - Chen, Yi-Ming AU - Chen YM AD - Department of Medical Research, Taichung Veterans General Hospital, Taichung, Taiwan. ymchen1@vghtc.gov.tw. AD - Division of Allergy, Immunology and Rheumatology, Department of Internal Medicine, Taichung Veterans General Hospital, Taichung, Taiwan. ymchen1@vghtc.gov.tw. AD - School of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan. ymchen1@vghtc.gov.tw. AD - Department of Post-Baccalaureate Medicine, College of Medicine, National Chung-Hsing University, Taichung, Taiwan. ymchen1@vghtc.gov.tw. AD - Graduate Institute of Clinical Medicine, College of Medicine, National Chung Hsing University, Taichung, Taiwan. ymchen1@vghtc.gov.tw. AD - Graduate Institute of Clinical Medicine, College of Medicine, National Chung Hsing University, Taichung, Taiwan. ymchen1@vghtc.gov.tw. LA - eng GR - NSTC- 113-2314-B-040-003/National Science and Technology Council/ GR - NSTC-111-2634-F-A49-014, NSTC-111-2218-E-039-001, NSTC-111-2314-B-075A-003-MY3/National Science and Technology Council/ GR - RD11216/Chung Shan Medical University, Taiwan/ GR - TCVGH-1127301C, TCVGH-1127302D, and TCVGH-YM1120110/Taichung Veterans General Hospital, Taiwan/ PT - Journal Article DEP - 20260804 PL - England TA - Arthritis Res Ther JT - Arthritis research & therapy JID - 101154438 RN - 0 (Biomarkers) RN - 0 (Janus Kinase Inhibitors) RN - 0 (Purines) RN - 87LA6FU830 (tofacitinib) RN - 0 (Pyrimidines) RN - 0 (Azetidines) RN - 0 (Pyrazoles) RN - 0 (Sulfonamides) RN - ISP4442I3Y (baricitinib) RN - 0 (Piperidines) RN - 4RA0KN46E0 (upadacitinib) RN - 0 (Heterocyclic Compounds, 3-Ring) SB - IM MH - Humans MH - *Arthritis, Rheumatoid/drug therapy/blood/metabolism MH - *Proteomics/methods MH - Female MH - *Biomarkers/blood MH - *Machine Learning MH - *Janus Kinase Inhibitors/therapeutic use MH - Male MH - Purines/therapeutic use MH - Pyrimidines/therapeutic use MH - Azetidines/therapeutic use MH - Middle Aged MH - Pyrazoles/therapeutic use MH - Prospective Studies MH - Sulfonamides/therapeutic use MH - Treatment Outcome MH - Piperidines/therapeutic use MH - Aged MH - Adult MH - Heterocyclic Compounds, 3-Ring OTO - NOTNLM OT - JAK inhibitor OT - Machine learning OT - Proteomic OT - Response OT - Rheumatoid arthritis COIS- Declarations. Ethics approval and consent to participate: The study procedures were approved by the Joint Institutional Review Board of Taichung Veterans General Hospital, Taiwan (CF21176A) and written informed consent was obtained from all participants. The study was carried out in accordance with the Declaration of Helsinki. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:00 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2025/08/27 00:00 [received] PHST- 2026/07/30 00:00 [accepted] PHST- 2026/10/30 01:00 [entrez] AID - 10.1186/s13075-026-03880-0 [pii] AID - 10.1186/s13075-026-03880-0 [doi] PST - epublish SO - Arthritis Res Ther. 2026 Aug 4;28(1):191. doi: 10.1186/s13075-026-03880-0. PMID- 42958312 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2662-7671 (Electronic) IS - 2662-7671 (Linking) VI - 26 IP - 1 DP - 2026 Aug 4 TI - The effects of root decay on the anti-tumor potential of Scutellariae Radix: an investigation using mass spectrometry imaging, network pharmacology and in vitro validation. LID - 328 [pii] LID - 10.1186/s12906-026-05457-5 [doi] AB - BACKGROUND: Scutellariae Radix, the dried root of Scutellaria baicalensis Georgi, is widely used in complementary medicine for its therapeutical effects to treat hepatitis, diarrhea and inflammation. It has been confirmed by modern research that Scutellariae Radix also exhibits anti-tumor properties. As S. baicalensis plants grow into older ages, some of the roots start to decay. The decayed Scutellariae Radix is believed to exhibit different pharmacological effects. It is essential to understand how root decay affects the chemical composition of Scutellariae Radix and, consequently, contributes to the alteration of pharmacological effects. METHODS: Mass spectrometry imaging (MSI) was adopted to map out the spatial distribution of natural compounds in the decayed roots of Scutellariae Radix. Compounds that showed varied contents between decayed and non-decayed tissues were further identified with liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Identified compounds exhibiting high gastrointestinal adsorption values and high drug-likeness values were subjected to network pharmacology analysis. The influence of chemical alteration on pharmacological effects was predicted. Predictions were validated through in vitro assays on cancer cell lines (A-549, BxPC-3, KGN, and SGC-7901). RESULTS: A majority of compounds was observed to show significantly decreased contents in the decayed tissues of Scutellariae Radix. The shared targets of 15 key components and cancer were highly enriched in PI3K-Akt signaling pathway and multiple organ-specific cancer pathways. Apigenin, presenting the highest degree value during the establishment of component-target network, was selected as the representative component for in vitro validation of anti-tumor properties. Apigenin was found to inhibit the proliferation of four different tumor cell lines in a dose-dependent manner. CONCLUSIONS: The chemical composition of Scutellariae Radix was closely correlated with root decay. The contents of numerous compounds showed sharp decline within the decayed tissues of roots. It was revealed by network pharmacology analysis and in vitro study that many compounds were potential anti-tumor compounds. These findings emphasized the need for separate quality standards for decayed and non-decayed roots. This research advances current exploration of medicinal plants and highlights the importance of quality control in medicinal plant use, contributing to the development of complementary medicine. CI - (c) 2026. The Author(s). FAU - Huang, Lieyan AU - Huang L AD - Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100006, P.R. China. FAU - Nie, Lixing AU - Nie L AD - National Institutes for Food and Drug Control, No. 31, Huatuo Avenue, Daxing Biomedical Complex, Daxing District, Beijing, 102629, P.R. China. nielixing@163.com. AD - WHO Collaborating Center for Herbal Medicine (CHN-139), Beijing, 102629, China. nielixing@163.com. FAU - Wu, Yanpei AU - Wu Y AD - National Institutes for Food and Drug Control, No. 31, Huatuo Avenue, Daxing Biomedical Complex, Daxing District, Beijing, 102629, P.R. China. FAU - Dong, Jing AU - Dong J AD - Shimadzu China Innovation Center, Beijing, 100020, P.R. China. FAU - Kang, Shuai AU - Kang S AD - National Institutes for Food and Drug Control, No. 31, Huatuo Avenue, Daxing Biomedical Complex, Daxing District, Beijing, 102629, P.R. China. AD - WHO Collaborating Center for Herbal Medicine (CHN-139), Beijing, 102629, China. FAU - Wu, Yanlin AU - Wu Y AD - National Institutes for Food and Drug Control, No. 31, Huatuo Avenue, Daxing Biomedical Complex, Daxing District, Beijing, 102629, P.R. China. wyljoe@126.com. FAU - Wei, Feng AU - Wei F AD - National Institutes for Food and Drug Control, No. 31, Huatuo Avenue, Daxing Biomedical Complex, Daxing District, Beijing, 102629, P.R. China. weifeng@nifdc.org.cn. AD - WHO Collaborating Center for Herbal Medicine (CHN-139), Beijing, 102629, China. weifeng@nifdc.org.cn. FAU - Ma, Shuangcheng AU - Ma S AD - Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100006, P.R. China. masc@chp.org.cn. AD - Chinese Pharmacopeia Commission, Building 11, Fahua Nanli, Tiyuguan Road, Dongcheng District, Beijing, 100061, China. masc@chp.org.cn. AD - State Key Laboratory of Drug Regulatory Science, Beijing, 102629, China. masc@chp.org.cn. LA - eng GR - Ref. No. ASCC/HDD/HLD/LET/2023/29/ASEAN-China Coorperation Fund (ACCF)/ GR - CSTB2025JXJL-YFX0029/Chongqing Special Fund for Performance Incentive and Guidance of Research Institutions/ GR - 2024SKLDRS0101/the National Key Laboratory of Pharmaceutical Regulatory Science Project/ PT - Journal Article DEP - 20260804 PL - England TA - BMC Complement Med Ther JT - BMC complementary medicine and therapies JID - 101761232 RN - 0 (Plant Extracts) RN - 0 (Antineoplastic Agents, Phytogenic) SB - IM MH - *Scutellaria baicalensis/chemistry MH - *Plant Roots/chemistry MH - Humans MH - Network Pharmacology MH - Cell Line, Tumor MH - *Plant Extracts/pharmacology/chemistry MH - Mass Spectrometry MH - Tandem Mass Spectrometry MH - *Antineoplastic Agents, Phytogenic/pharmacology/chemistry OTO - NOTNLM OT - In vitro validation OT - Scutellaria baicalensis Georgi OT - Mass spectrometry imaging OT - Network pharmacology OT - Phytochemicals COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 01:00 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2025/03/25 00:00 [received] PHST- 2026/06/22 00:00 [accepted] PHST- 2026/10/30 01:00 [entrez] AID - 10.1186/s12906-026-05457-5 [pii] AID - 10.1186/s12906-026-05457-5 [doi] PST - epublish SO - BMC Complement Med Ther. 2026 Aug 4;26(1):328. doi: 10.1186/s12906-026-05457-5. PMID- 42958307 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1749-7922 (Electronic) IS - 1749-7922 (Linking) VI - 21 IP - 1 DP - 2026 Jul 24 TI - WSES-PSOGI-SICO evidence-infomed guidelines on surgical emergencies in patients with peritoneal surface malignancies: a systematic review, meta-analysis, and structured expert consensus. LID - 60 [pii] LID - 10.1186/s13017-026-00718-7 [doi] AB - BACKGROUND: Peritoneal surface malignancies (PSM) are primary or secondary neoplastic deposits involving the peritoneum and are commonly associated with colorectal, gastric, appendiceal, gynecological, pancreaticobiliary, and other intra-abdominal or extra-abdominal cancers. Malignant bowel obstruction (MBO) is one of the most frequent and clinically consequential emergency presentations in this population, yet diagnostic and therapeutic pathways remain heterogeneous. We aimed to develop evidence-informed recommendations for emergency management of MBO in patients with PSM by integrating a systematic review, meta-analysis and a structured international expert consensus process. METHODS: This systematic review, meta-analysis, and Delphi consensus followed PRISMA, Cochrane, and relevant MOOSE reporting principles. PubMed/MEDLINE and the Cochrane Library were searched from inception to June 2025 (English language). Five predefined PICO/PIRT questions addressed: (1) the diagnostic role of CT, plain abdominal X-ray, or Gastrografin-enhanced X-ray in PSM-related MBO; (2) nonoperative medical management versus surgical or endoscopic therapy; (3) surgical versus endoscopic treatment after failure of nonoperative management; (4) bowel resection versus stoma or bypass in surgical candidates; and (5) endoscopic stenting versus endoscopic ultrasound-guided bypass with lumen-apposing metal stents in endoscopic candidates. Study selection and data extraction were performed independently and in duplicate. Risk of bias was assessed with ROBINS-I for comparative non-randomized studies and MINORS for non-comparative studies; certainty of evidence was rated with GRADE. Random-effects meta-analyses (REML) produced odds ratios (OR), mean differences (MD), pooled means, or pooled proportions with 95% confidence intervals (CI); heterogeneity was summarized using I2, tau2, and 95% prediction intervals. Evidence profiles informed statements voted on by the WSES-PSOGI-SICO expert panel during the 2025 WSES Congress. RESULTS: No comparative diagnostic study evaluated abdominal X-ray or Gastrografin-enhanced X-ray in PSM-related MBO; two small retrospective CT-based series supported contrast-enhanced CT for mapping obstruction and excluding urgent complications, but no diagnostic meta-analysis was possible. For medical versus procedural treatment, medical management was associated with lower morbidity (OR 0.16, 95% CI 0.07-0.39) and shorter length of stay (MD - 9.48 days, 95% CI - 12.65 to -6.32), while success, mortality, and overall survival remained inconclusive. For surgery versus endoscopy, surgery showed higher clinical success (OR 2.57, 95% CI 1.47-4.50), whereas endoscopy shortened hospital stay (MD 3.35 days, 95% CI 1.46-5.23); mortality did not differ (OR 1.02, 95% CI 0.70-1.49). Among surgical options, resection showed a signal toward higher perioperative mortality than bypass or ostomy (OR 3.55, 95% CI 1.45-8.68). Endoscopic stenting showed a pooled clinical success rate of 0.71 (95% CI 0.62-0.79), whereas evidence on LAMS was limited to one retrospective study. All guideline statements reached 100% agreement among voting participants. CONCLUSIONS: Despite the low certainty of available evidence, integration of systematic review findings with expert consensus provides a structured international framework to support decision-making in emergency presentations of PSM-related MBO. These consensus-based recommendations are intended to assist clinicians in harmonizing care across peripheral and referral hospitals, while emphasizing individualized multidisciplinary judgement and the need for future higher-quality evidence. CI - (c) 2026. The Author(s). FAU - Fugazzola, Paola AU - Fugazzola P AD - General Surgery Department, Fondazione IRCCS Policlinico San Matteo, Via Golgi 19, 27100, Pavia, Italy. paola.fugazzola@gmail.com. FAU - Granieri, Stefano AU - Granieri S AD - Acute Care and Trauma Surgery, ASST GOM Niguarda, Milan, Italy. FAU - Abu-Zidan, Fikri AU - Abu-Zidan F AD - Department of Surgery, College of Medicine and Health Sciences, United Arab Emirates University, Al‑Ain, United Arab Emirates. FAU - Allievi, Niccolo AU - Allievi N AD - General Surgery Department, ASST Papa Giovanni XXIII, Bergamo, Italy. FAU - Biffl, Walter L AU - Biffl WL AD - Division of Trauma/Acute Care Surgery, Scripps Clinic Medical Group, La Jolla, San Diego, CA, USA. FAU - Catena, Fausto AU - Catena F AD - Dept of General and Emergency Surgery, Bufalini Hospital AUSL Romagna, Cesena, Italy. AD - Dept of Medical and Surgical Sciences Alma Mater, Studiorum University of Bologna, Bologna, Italy. FAU - Celotti, Andrea AU - Celotti A AD - ASST Cremona - UOC General Surgery, Cremona, Italy. FAU - Ceresoli, Marco AU - Ceresoli M AD - General and Emergency Surgery Department, Fondazione IRCCS San Gerardo Dei Tintori, Monza, Italy. AD - School of Medicine and Surgery, University of Milano-Bicocca, Milan, Italy. FAU - Cobianchi, Lorenzo AU - Cobianchi L AD - General Surgery Department, Fondazione IRCCS Policlinico San Matteo, Via Golgi 19, 27100, Pavia, Italy. AD - Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy. AD - Collegium Medicum, SAN University, Lodz, Poland. FAU - Coccolini, Federico AU - Coccolini F AD - General Emergency and Trauma Surgery Department, Pisa University Hospital, Pisa, Italy. FAU - Dagnoni, Andrea AU - Dagnoni A AD - General Surgery Department, Fondazione IRCCS Policlinico San Matteo, Via Golgi 19, 27100, Pavia, Italy. FAU - Mas, Francesca Dal AU - Mas FD AD - Collegium Medicum, SAN University, Lodz, Poland. AD - Venice School of Management, Ca' Foscari University of Venice, 30123, Venice, Italy. FAU - Di Giorgio, Andrea AU - Di Giorgio A AD - Surgical Unit of Peritoneum and Retroperitoneum, Division of General Surgery, Fondazione Policlinico Universitario A. Gemelli - IRCCS, Rome, Italy. FAU - Filios, Rebecca AU - Filios R AD - General Surgery Department, Fondazione IRCCS Policlinico San Matteo, Via Golgi 19, 27100, Pavia, Italy. FAU - Frassini, Simone AU - Frassini S AD - General Surgery Department, Fondazione IRCCS Policlinico San Matteo, Via Golgi 19, 27100, Pavia, Italy. AD - Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy. FAU - Ghaly, Ahmed AU - Ghaly A AD - General Surgery Department, Fondazione IRCCS Policlinico San Matteo, Via Golgi 19, 27100, Pavia, Italy. FAU - Ioannidis, Orestis AU - Ioannidis O AD - 4th Department of Surgery, General Hospital "George Papanikolaou", Aristotle University of Thessaloniki, 57010, Exochi, Greece. FAU - Isik, Arda AU - Isik A AD - General Surgery, Istanbul Medeniyet University, Kadikoy, Istanbul, Turkey. FAU - Markou-Pappas, Nikolaos AU - Markou-Pappas N AD - General Surgery Department, Fondazione IRCCS Policlinico San Matteo, Via Golgi 19, 27100, Pavia, Italy. FAU - Perrina, Daniele AU - Perrina D AD - Dept of General and Emergency Surgery, Bufalini Hospital AUSL Romagna, Cesena, Italy. FAU - Pisano, Michele AU - Pisano M AD - General Surgery Department, ASST Papa Giovanni XXIII, Bergamo, Italy. FAU - Podda, Mauro AU - Podda M AD - Department of Surgical Science, University of Cagliari, Cagliari, Italy. FAU - Seshadri, Ramakrishan AU - Seshadri R AD - Department of Surgical Oncology, Cancer Institute (WIA), Chennai, India. FAU - Sommariva, Antonio AU - Sommariva A AD - Surgical Oncology of Digestive Tract, Veneto Institute of Oncology IOV-IRCCS, Padua, Italy. FAU - Tomasoni, Matteo AU - Tomasoni M AD - General Surgery Department, Fondazione IRCCS Policlinico San Matteo, Via Golgi 19, 27100, Pavia, Italy. FAU - Vaira, Marco AU - Vaira M AD - Programma Di Chirurgia Tumori del Peritoneo, UOC Chirurgia d'urgenza Ed Oncologica, Azienda Ospedaliero-Universitaria Di Modena, Modena, Italy. FAU - Vigano, Jacopo AU - Vigano J AD - General Surgery Department, Fondazione IRCCS Policlinico San Matteo, Via Golgi 19, 27100, Pavia, Italy. FAU - Ward, Erin AU - Ward E AD - Huntsman Cancer Institute, University of Utah Health, Salt Lake City, UT, USA. FAU - Giuffrida, Mario AU - Giuffrida M AD - Department of General Surgery, AUSL Piacenza, Piacenza, Italy. FAU - Chierici, Andrea AU - Chierici A AD - Digestive Surgery and Liver Transplant Unit, CHU de Nice, Nice, France. FAU - Yigitler, Cengizhan AU - Yigitler C AD - MEDICANA International Hospital, Istanbul, Turkey. FAU - Wexner, Steven AU - Wexner S AD - Department of Surgery, Georgetown University/MedStar Health in Washington DC, Washington, DC, USA. FAU - Sugarbaker, Paul AU - Sugarbaker P AD - Program in Peritoneal Surface Malignancy, Washington Cancer Institute, Washington, DC, USA. FAU - Ansaloni, Luca AU - Ansaloni L AD - General Surgery Department, Fondazione IRCCS Policlinico San Matteo, Via Golgi 19, 27100, Pavia, Italy. AD - Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy. LA - eng PT - Journal Article PT - Meta-Analysis PT - Review PT - Systematic Review DEP - 20260724 PL - England TA - World J Emerg Surg JT - World journal of emergency surgery : WJES JID - 101266603 SB - IM MH - Humans MH - *Peritoneal Neoplasms/surgery/complications MH - *Intestinal Obstruction/surgery/etiology MH - Emergencies OTO - NOTNLM OT - Carcinomatosis OT - Malignant bowel obstruction OT - Peritoneal metastases OT - Peritoneal surface malignancies COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 00:59 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2025/12/23 00:00 [received] PHST- 2026/07/18 00:00 [accepted] PHST- 2026/10/30 00:59 [entrez] AID - 10.1186/s13017-026-00718-7 [pii] AID - 10.1186/s13017-026-00718-7 [doi] PST - epublish SO - World J Emerg Surg. 2026 Jul 24;21(1):60. doi: 10.1186/s13017-026-00718-7. PMID- 42958305 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1749-799X (Electronic) IS - 1749-799X (Linking) VI - 21 IP - 1 DP - 2026 Aug 3 TI - Association of a structured perioperative nursing intervention pathway with recovery and psychological outcomes in patients undergoing lumbar spine surgery: a non-concurrent controlled study : Author. LID - 620 [pii] LID - 10.1186/s13018-026-07128-0 [doi] AB - BACKGROUND: Degenerative lumbar spinal stenosis (DLSS) is a common condition associated with pain, functional limitation, and psychological distress. Although lumbar fusion surgery can relieve symptoms, postoperative recovery may be influenced by perioperative nursing care. This study aimed to evaluate the association between a structured perioperative nursing intervention pathway and recovery and psychological outcomes in patients undergoing lumbar spine surgery. METHODS: The final complete-case analysis of this non-concurrent single-center study included 140 patients with DLSS who underwent posterior lumbar interbody fusion (PLIF) or transforaminal lumbar interbody fusion (TLIF). Patients admitted from May to August 2024 were assigned to the control group (n = 70) and received routine nursing care, whereas those admitted from September 2024 to April 2025 were assigned to the intervention group (n = 70) and received a structured perioperative nursing intervention in addition to routine care. Clinical outcomes, including pain assessed by the Visual Analog Scale (VAS), functional disability assessed by the Oswestry Disability Index (ODI), and psychological status assessed by the Hospital Anxiety and Depression Scale (HADS), were evaluated preoperatively, on postoperative day 3 (POD3), at discharge, and at 1, 3, 6, and 12 months postoperatively. The primary outcome was VAS pain score at discharge, whereas other time-specific comparisons of VAS, ODI, HADS-A, and HADS-D were considered exploratory. No formal adjustment for multiple comparisons was applied to these exploratory time-specific analyses. RESULTS: Baseline demographic and clinical characteristics were comparable between groups. The intervention group had a shorter length of hospital stay than the control group (10.26 +/- 4.86 vs. 12.31 +/- 4.25 days, P = 0.009). At POD3, no between-group differences were observed in VAS, ODI, HADS-A, or HADS-D scores. For the prespecified primary outcome, the intervention group had a lower VAS score at discharge (3.57 +/- 2.12 vs. 4.74 +/- 2.42, P = 0.003). Exploratory unadjusted analyses showed selected between-group differences in functional and psychological scores during the first 6 postoperative months; these findings should be interpreted cautiously because no multiplicity adjustment was applied. CONCLUSIONS: In this single-center, non-randomized, non-concurrent controlled study, the structured perioperative nursing intervention pathway was associated with a lower VAS pain score at the clinically defined discharge time point. Exploratory analyses suggested possible favorable functional and psychological trajectories at selected mid-term follow-up points; however, these findings should be considered hypothesis-generating because of the sequential cohort design and multiple unadjusted comparisons. Further prospective randomized studies are needed to confirm these associations. CI - (c) 2026. The Author(s). FAU - Song, Xinyan AU - Song X AD - Department of Spine Surgery, the Third Hospital of Hebei Medical University, Shijiazhuang, 050051, China. FAU - Wang, Ruiling AU - Wang R AD - Department of Spine Surgery, the Third Hospital of Hebei Medical University, Shijiazhuang, 050051, China. FAU - Ding, Jia AU - Ding J AD - Department of Spine Surgery, the Third Hospital of Hebei Medical University, Shijiazhuang, 050051, China. 403277980@qq.com. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - J Orthop Surg Res JT - Journal of orthopaedic surgery and research JID - 101265112 SB - IM MH - Humans MH - Female MH - *Lumbar Vertebrae/surgery MH - Male MH - *Spinal Fusion/psychology MH - Aged MH - *Spinal Stenosis/surgery/psychology MH - Middle Aged MH - *Recovery of Function MH - Treatment Outcome MH - *Perioperative Nursing/methods OTO - NOTNLM OT - Lumbar spine surgery OT - Nursing intervention OT - Perioperative nursing OT - Psychological outcomes OT - Rehabilitation COIS- Declarations. Ethical approval: This study was approved by the Ethics Committee of the Third Hospital of Hebei Medical University and all subjects enrolled provided informed consent. Consent for publication: Written informed consent was obtained from the patient for publication of anonymized clinical images. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 00:59 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/04/07 00:00 [received] PHST- 2026/07/17 00:00 [accepted] PHST- 2026/10/30 00:59 [entrez] AID - 10.1186/s13018-026-07128-0 [pii] AID - 10.1186/s13018-026-07128-0 [doi] PST - epublish SO - J Orthop Surg Res. 2026 Aug 3;21(1):620. doi: 10.1186/s13018-026-07128-0. PMID- 42958303 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1749-799X (Electronic) IS - 1749-799X (Linking) VI - 21 IP - 1 DP - 2026 Aug 1 TI - Does minimally invasive plating reduce supraclavicular nerve injury compared with nerve-preserving open plating in clavicle fractures? A systematic review and meta-analysis. LID - 619 [pii] LID - 10.1186/s13018-026-07140-4 [doi] AB - BACKGROUND: Minimally invasive plate osteosynthesis (MIPO) has been increasingly used for midshaft clavicle fractures, with proposed advantages in reducing soft tissue injury and nerve-related complications. However, whether MIPO provides additional benefit over nerve-preserving open reduction and internal fixation (ORIF) remains unclear. METHODS: A systematic review and meta-analysis was conducted according to PRISMA guidelines. Studies comparing MIPO with nerve-preserving ORIF for midshaft clavicle fractures were included. Primary outcome was supraclavicular nerve (SCN)-related sensory disturbance. Secondary outcomes included functional scores, operative time, blood loss, fracture union time, return to activity, and complications. Random-effects models were used, and prespecified subgroup analyses were performed based on incision strategy, SCN identification, and study design. RESULTS: A total of 10 studies involving 500 patients (3 randomized controlled trials and 7 retrospective studies) were included. MIPO was associated with a lower incidence of SCN-related sensory disturbance compared with nerve-preserving ORIF (RR 0.34, 95% CI 0.23-0.50), with no observed heterogeneity. This finding was consistent across subgroup analyses, including randomized controlled trials. Functional outcomes, fracture union time, and overall complication rates were generally comparable between techniques. MIPO was associated with reduced intraoperative blood loss and shorter time to return to activity, although these findings were based on a limited number of studies and showed heterogeneity. Operative time tended to be shorter with MIPO but demonstrated substantial variability across studies. CONCLUSIONS: MIPO is associated with a lower incidence of SCN-related sensory disturbance compared with nerve-preserving ORIF. This advantage appears consistent across variations in surgical technique, while other outcomes are generally comparable between techniques. CI - (c) 2026. The Author(s). FAU - Chou, Chia-You AU - Chou CY AD - Department of Orthopedics, Chi Mei Medical Center, No. 901, Zhonghua Rd., Yongkang Dist., Tainan City, 71004, Taiwan, ROC. FAU - Chien, Chi-Sheng AU - Chien CS AD - Department of Orthopedics, Chi Mei Medical Center, No. 901, Zhonghua Rd., Yongkang Dist., Tainan City, 71004, Taiwan, ROC. FAU - Lin, Wei-Ting AU - Lin WT AD - Department of Orthopedics, Chi Mei Medical Center, No. 901, Zhonghua Rd., Yongkang Dist., Tainan City, 71004, Taiwan, ROC. aapriliaa@gmail.com. AD - Department of Mechanical Engineering, Southern Taiwan University of Science and Technology, Tainan City, 710, Taiwan, ROC. aapriliaa@gmail.com. LA - eng PT - Comparative Study PT - Journal Article PT - Meta-Analysis PT - Systematic Review DEP - 20260801 PL - England TA - J Orthop Surg Res JT - Journal of orthopaedic surgery and research JID - 101265112 SB - IM MH - Humans MH - *Clavicle/injuries/surgery MH - *Fracture Fixation, Internal/methods/adverse effects MH - *Fractures, Bone/surgery MH - *Minimally Invasive Surgical Procedures/methods/adverse effects MH - *Bone Plates MH - Open Fracture Reduction/methods/adverse effects MH - Treatment Outcome MH - *Peripheral Nerve Injuries/prevention & control/etiology MH - Postoperative Complications/prevention & control/etiology COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Clinical trial number: Not applicable. Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 00:59 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/04/29 00:00 [received] PHST- 2026/07/22 00:00 [accepted] PHST- 2026/10/30 00:59 [entrez] AID - 10.1186/s13018-026-07140-4 [pii] AID - 10.1186/s13018-026-07140-4 [doi] PST - epublish SO - J Orthop Surg Res. 2026 Aug 1;21(1):619. doi: 10.1186/s13018-026-07140-4. PMID- 42958300 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1745-6215 (Electronic) IS - 1745-6215 (Linking) VI - 27 IP - 1 DP - 2026 Aug 4 TI - Effects of mechanical thrombectomy combined with anticoagulation vs anticoagulation monotherapy for acute intermediate-high risk pulmonary embolism: study protocol for a randomized controlled trial (CAPE study, ChiCTR2500095253, https://www.medicalresearch.org.cn/clinicalResearch/researchInfo?id=c57630b7-51ec-4b24-83e1-85b9c0a835d6). LID - 636 [pii] LID - 10.1186/s13063-026-09932-9 [doi] AB - BACKGROUND: Treatment of intermediate-high-risk pulmonary embolism is still debated. Interventional therapy is being increasingly widely applied in clinical practice. Numerous studies have confirmed that interventional therapy is effective. Both catheter-directed thrombolysis (CDT) and mechanical thrombectomy (MT) showed improvement in some efficacy outcomes compared with anticoagulation monotherapy. A recent study showed that MT has lower rates of clinical deterioration and/or bailout and postprocedural intensive care unit use compared with CDT, with no difference in mortality or bleeding, which makes the application of MT in clinical practice increasingly widespread. Recently, STORM-PE, the first randomized controlled trial to test the efficacy and evaluate the safety of MT combined with anticoagulation compared with anticoagulation monotherapy for the treatment of intermediate-high-risk pulmonary embolism, showed that treatment with MT combined with anticoagulation resulted in a significantly greater reduction in right ventricular/left ventricular (RV/LV) diameter ratio at 48 h versus anticoagulation monotherapy, with comparably low rates of major adverse events in both treatment arms. However, whether MT combined with anticoagulation is more effective than anticoagulation monotherapy needs more evidence, especially in Asian populations. METHODS: The CAPE study is a currently enrolling national multicenter randomized controlled trial (RCT) comparing MT with MPA 8F guiding catheter [Cordis (Shanghai) Medical Device Co., Ltd] combined with anticoagulation vs anticoagulation monotherapy. A total of 156 patients with acute intermediate-high-risk pulmonary embolism stratified by the classification of the latest guideline of European Society of Cardiology (ESC) will be enrolled and randomized in a 1:1 ratio. The primary efficacy endpoint was reduction of RV/LV diameter ratio assessed by computed tomographic angiography (CTPA) at 48 h. The secondary efficacy endpoint was the incidence of chronic thromboembolic pulmonary disease including chronic thromboembolic pulmonary hypertension. The primary safety endpoint was serious adverse events including major bleeding through 3 months visit. DISCUSSION: CAPE is a RCT to compare the short and long efficacy of MT combined with anticoagulation and anticoagulation monotherapy for acute intermediate-high pulmonary embolism. The result will give more evidence for selection of different strategies for physicians. TRIAL REGISTRATION: CAPE study is registered in Chinese Clinical Trial Registry identifier (ChiCTR2500095253, https://www.medicalresearch.org.cn/clinicalResearch/researchInfo?id=c57630b7-51ec-4b24-83e1-85b9c0a835d6 ). Registered on November 26 2024. CI - (c) 2026. The Author(s). FAU - Huang, Qiang AU - Huang Q AD - National Center for Respiratory Medicine, Beijing, 100029, China. AD - State Key Laboratory of Respiratory Health and Multimorbidity, Beijing, 100029, China. AD - National Clinical Research Center for Respiratory Diseases, National Clinical Research Center for Respiratory Diseases, 100029, China. AD - Institute of Respiratory Medicine, Chinese Academy of Medical Sciences, Beijing, 100029, China. AD - Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, China-Japan Friendship Hospital, Beijing, 100029, China. FAU - Feng, Lin AU - Feng L AD - Evidence-Based Medicine Center, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China. FAU - Liu, Lin AU - Liu L AD - Department of Respiratory and Critical Care Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, P.R. China. AD - Beijing Institute of Respiratory Medicine, Beijing, 100020, P.R. China. FAU - Li, Yi-Shan AU - Li YS AD - Department of Respiratory and Critical Care Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, P.R. China. AD - Beijing Institute of Respiratory Medicine, Beijing, 100020, P.R. China. FAU - Guo, Wei AU - Guo W AD - Department of Pulmonary and Critical Care Medicine, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing, China. FAU - Li, Fa-Jiu AU - Li FJ AD - Department of Pulmonary and Critical Care Medicine, The Sixth Hospital of Wuhan, Affiliated Hospital of Jianghan University, Wuhan, Hubei, China. FAU - Li, Li AU - Li L AD - Department of Respiratory Medicine, Haihe Hospital, Tianjin University, Tianjin, China. FAU - Ren, Yunong AU - Ren Y AD - Department of Respiratory and Critical Care Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, P.R. China. AD - Beijing Institute of Respiratory Medicine, Beijing, 100020, P.R. China. FAU - Zhou, Chuan-Guo AU - Zhou CG AD - Department of Interventional Radiology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, P.R. China. FAU - Zhang, Shuai AU - Zhang S AD - National Center for Respiratory Medicine, Beijing, 100029, China. AD - State Key Laboratory of Respiratory Health and Multimorbidity, Beijing, 100029, China. AD - National Clinical Research Center for Respiratory Diseases, National Clinical Research Center for Respiratory Diseases, 100029, China. AD - Institute of Respiratory Medicine, Chinese Academy of Medical Sciences, Beijing, 100029, China. AD - Department of Pulmonary and Critical Care Medicine, Center of Respiratory Medicine, China-Japan Friendship Hospital, Beijing, 100029, China. FAU - Zhang, Meng AU - Zhang M AD - Department of Pulmonary and Critical Care Medicine, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing, China. FAU - Li, Cheng-Hong AU - Li CH AD - Department of Pulmonary and Critical Care Medicine, The Sixth Hospital of Wuhan, Affiliated Hospital of Jianghan University, Wuhan, Hubei, China. FAU - Li, Hong-Wei AU - Li HW AD - Department of Respiratory Medicine, Haihe Hospital, Tianjin University, Tianjin, China. FAU - Yang, Qi AU - Yang Q AD - Department of Radiology, Beijing Chao-Yang Hospital, Capital Medical University, Key Lab of Medical Engineering for Cardiovascular Disease, Ministry of Education, Beijing, China. yangyangqiqi@gmail.com. FAU - Yang, Yuan-Hua AU - Yang YH AD - Department of Respiratory and Critical Care Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, P.R. China. yyh1031@sina.com. AD - Beijing Institute of Respiratory Medicine, Beijing, 100020, P.R. China. yyh1031@sina.com. FAU - Li, Ji-Feng AU - Li JF AUID- ORCID: 0000-0002-0418-6151 AD - Department of Respiratory and Critical Care Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, P.R. China. lijifeng@ccmu.edu.cn. AD - Beijing Institute of Respiratory Medicine, Beijing, 100020, P.R. China. lijifeng@ccmu.edu.cn. LA - eng PT - Clinical Trial Protocol PT - Journal Article DEP - 20260804 PL - England TA - Trials JT - Trials JID - 101263253 RN - 0 (Anticoagulants) SB - IM MH - Humans MH - *Pulmonary Embolism/therapy/diagnostic imaging MH - *Anticoagulants/therapeutic use/adverse effects MH - Treatment Outcome MH - Randomized Controlled Trials as Topic MH - *Thrombectomy/adverse effects/methods MH - Multicenter Studies as Topic MH - Time Factors MH - Combined Modality Therapy MH - Risk Factors MH - Acute Disease MH - China OTO - NOTNLM OT - Anticoagulation OT - Intermediate-high risk OT - Mechanical thrombectomy OT - Pulmonary embolism OT - RCT COIS- Declarations. Ethics approval and consent to participate 30: Favorable ethical approval for the trial was granted by Ethics Committee of Beijing Chao-Yang Hospital (Institution of PI) on 4 November 2024 (2024-Research-884). Written informed consent to participate will be obtained from all participants before they are enrolled in the trial. Consent for publication: This manuscript does not contain individual personal data from participants. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 00:59 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/01/30 00:00 [received] PHST- 2026/07/25 00:00 [accepted] PHST- 2026/10/30 00:59 [entrez] AID - 10.1186/s13063-026-09932-9 [pii] AID - 10.1186/s13063-026-09932-9 [doi] PST - epublish SO - Trials. 2026 Aug 4;27(1):636. doi: 10.1186/s13063-026-09932-9. PMID- 42958297 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1472-6947 (Electronic) IS - 1472-6947 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Retrospective study: preoperative MRI features for predicting microvascular decompression outcomes in trigeminal neuralgia. LID - 443 [pii] LID - 10.1186/s12911-026-03745-1 [doi] AB - OBJECTIVE: To develop and internally validate machine learning models based on preoperative magnetic resonance imaging (MRI) features for predicting MVD treatment outcomes in TN patients. METHODS: This retrospective single-center study included 100 patients with TN who underwent primary MVD surgery between January 2020 and December 2024. Preoperative high-resolution MRI sequences including 3D-T2 and time-of-flight magnetic resonance angiography (TOF-MRA) were analyzed. Imaging features included neurovascular compression (NVC) characteristics, nerve morphology, anatomical measurements, and radiomics features. Multiple machine learning algorithms including logistic regression, support vector machine, random forest, and gradient boosting were developed and internally validated using 10-fold cross-validation. All feature preprocessing and selection steps (correlation pruning, univariate analysis, recursive feature elimination) were performed within each training fold of the cross-validation framework to eliminate information leakage. The effective number of predictors after selection and event per predictor (EPP) ratio were calculated to assess overfitting risk associated with class imbalance. RESULTS: Complete pain relief was achieved in 93% of patients at final follow-up. The gradient boosting model demonstrated the best predictive performance with an area under the curve (AUC) of 0.847 (95% CI: 0.752-0.925), accuracy of 82.3%, sensitivity of 78.6%, and specificity of 87.5%. Calibration analysis for the gradient boosting model demonstrated a calibration intercept of 0.08 and calibration slope of 0.94, with a Brier score of 0.127 (95% CI: 0.089-0.176), indicating excellent agreement between predicted and observed outcomes. The most important predictive features included NVC severity grade (importance: 0.234), neurovascular angle (importance: 0.187), nerve atrophy presence (importance: 0.156), arterial compression type (importance: 0.142), and proximal NVC location (importance: 0.128). This univariate significance of NVC severity grade (p = 0.003) was consistent with its top ranking in SHAP feature importance analysis, validating the robustness of this key predictive feature. Patients with severe NVC (grade 2) had significantly higher success rates compared to mild NVC (96.4% vs. 76.2%, p = 0.003). CONCLUSION: Machine learning models integrating preoperative MRI features can accurately predict MVD surgical outcomes in TN patients. SHapley Additive exPlanations (SHAP) values provide minimal interpretability for these models, offering basic insight into feature contribution without establishing causal relationships or actionable clinical causality. These predictive models may facilitate personalized treatment planning, improve patient counseling, and optimize surgical decision-making within the evolving framework of precision neurosurgical care and Enhanced Recovery After Surgery (ERAS) protocols. Prospective external validation in multi-center cohorts is urgently required to confirm model generalizability before routine clinical implementation. CI - (c) 2026. The Author(s). FAU - Chen, Fei AU - Chen F AD - Department of Neurosurgery, Jining No.1 People's Hospital, No. 269 Mengzi Avenue, High Tech Zone, Jining City, Shandong Province, 272073, China. FAU - Shang, Dandan AU - Shang D AD - Department of Neurosurgery, Jining No.1 People's Hospital, No. 269 Mengzi Avenue, High Tech Zone, Jining City, Shandong Province, 272073, China. FAU - Sun, Dengbin AU - Sun D AD - Department of Neurosurgery, Jining No.1 People's Hospital, No. 269 Mengzi Avenue, High Tech Zone, Jining City, Shandong Province, 272073, China. FAU - Wang, Zhaoping AU - Wang Z AD - Department of Neurosurgery, Jining No.1 People's Hospital, No. 269 Mengzi Avenue, High Tech Zone, Jining City, Shandong Province, 272073, China. wangZP7819@163.com. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - BMC Med Inform Decis Mak JT - BMC medical informatics and decision making JID - 101088682 SB - IM MH - Humans MH - *Trigeminal Neuralgia/surgery/diagnostic imaging MH - Retrospective Studies MH - *Magnetic Resonance Imaging/methods MH - Female MH - Predictive Learning Models MH - *Microvascular Decompression Surgery MH - Male MH - Middle Aged MH - *Machine Learning MH - Aged MH - Treatment Outcome MH - Random Forest MH - Radiomics MH - Prediction Algorithms OTO - NOTNLM OT - Machine learning OT - Magnetic resonance imaging OT - Microvascular decompression OT - Radiomics OT - Trigeminal neuralgia COIS- Declarations. Ethics approval: This study was approved by the Ethics Committee of Jining First People's Hospital (Approval Number: 2025-IIT-Fast 162, Approval Date: 2025-12-12), which explicitly covered the secondary use of existing clinical data, imaging studies, and radiomics feature extraction. Informed consent was waived for this retrospective study in accordance with the 1964 Helsinki Declaration and its later amendments. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 00:59 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2025/11/20 00:00 [received] PHST- 2026/04/20 00:00 [accepted] PHST- 2026/10/30 00:59 [entrez] AID - 10.1186/s12911-026-03745-1 [pii] AID - 10.1186/s12911-026-03745-1 [doi] PST - epublish SO - BMC Med Inform Decis Mak. 2026 Aug 3;26(1):443. doi: 10.1186/s12911-026-03745-1. PMID- 42958296 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1465-542X (Electronic) IS - 1465-5411 (Linking) VI - 28 IP - 1 DP - 2026 Aug 2 TI - CK2 targeting potentiates antineoplastic effects of dual HER2/EGFR inhibitor lapatinib and attenuates growth factor-induced epithelial-mesenchymal transition. LID - 184 [pii] LID - 10.1186/s13058-026-02356-y [doi] AB - BACKGROUND: CK2 is a pleiotropic, heterotetrameric kinase elevated in numerous cancers and, as such, has garnered much attention as a target for novel antineoplastic agents. Currently, silmitasertib, a selective CK2 inhibitor, has an orphan designation for medulloblastoma and neuroblastoma. Tissue-specific overexpression of the catalytic subunit, CK2alpha, is sufficient to drive the development of mammary tumors in mice, underlying its potential role in breast oncogenesis. Our lab has demonstrated that CK2beta protein expression is associated with endocrine resistance and that targeted disruption of CK2 activity results in ERalpha proteolysis. Among the factors driving endocrine resistance is overexpression of HER2/EGFR, which contributes to both proliferation and phenotypic plasticity in ER(+) breast cancer. Here, we sought to determine the impact of CK2 targeting on HER2/EGFR signaling, phenotypic plasticity, and cell proliferation in endocrine-sensitive and endocrine-resistant breast cancer. Furthermore, we explored the potential of combined lapatinib and CK2-based therapies in decreasing breast cancer proliferation and epithelial-mesenchymal transition (EMT). METHODS: CK2 subunit expression and function were investigated in ERalpha-mutant, tamoxifen-resistant MCF-7 Y537S breast cancer cells and additional breast cancer subtypes. CK2 was inhibited pharmacologically using CX-4945 or genetically via shRNA knockdown of CK2beta. Cell proliferation, spheroid growth, migration, and EMT markers were assessed using live-cell imaging, wound-healing assays, immunoblotting, and qRT-PCR. Publicly available patient datasets were analyzed to evaluate the prognostic relevance of CSNK2B expression. RESULTS: CK2beta expression was significantly upregulated in ERalpha-mutant breast cancer cells and patient tumors. Pharmacological inhibition or genetic depletion of CK2beta suppressed proliferation, spheroid formation, and migration, accompanied by reduced ERK phosphorylation and cyclin D1 expression. CK2beta inhibition also attenuated EMT, marked by decreased N-cadherin, vimentin, and EMT transcription factors, while enhancing E-cadherin expression. High CSNK2B expression correlated with poorer relapse-free survival, particularly in ERalpha(+) and HER2-E breast cancers. Notably, CK2beta inhibition sensitized breast cancer cells to HER2-targeted therapy. CONCLUSIONS: CK2 is a critical regulator of endocrine-resistant breast cancer progression, promoting proliferation, EMT, and therapeutic resistance through ERK/cyclin D1 signaling. Inhibition of CK2 potentiated the effects of lapatinib across several cell lines. Furthermore, shRNA-mediated knockdown of the CK2beta subunit phenocopies pharmacological disruption of CK2 and highlights the significance of the regulatory subunit in breast cancer cell biology. Targeting CK2, and, more selectively, CK2beta represent a promising approach to overcome endocrine resistance and enhance responsiveness to HER2-directed treatments. CI - (c) 2026. The Author(s). FAU - Robinson, DeZha AU - Robinson D AD - College of Pharmacy, Xavier University of Louisiana, 1 Drexel Dr, New Orleans, LA, 70125, USA. FAU - Kim, Hogyoung AU - Kim H AD - College of Pharmacy, Xavier University of Louisiana, 1 Drexel Dr, New Orleans, LA, 70125, USA. FAU - Selico-Dunn, Alexandra AU - Selico-Dunn A AD - College of Pharmacy, Xavier University of Louisiana, 1 Drexel Dr, New Orleans, LA, 70125, USA. FAU - Elkins, Emma AU - Elkins E AD - College of Pharmacy, Xavier University of Louisiana, 1 Drexel Dr, New Orleans, LA, 70125, USA. FAU - Kim, Sichan AU - Kim S AD - College of Arts and Sciences, Xavier University of Louisiana, 1 Drexel Dr, New Orleans, LA, 70125, USA. FAU - Kim, Sihyoung AU - Kim S AD - College of Arts and Sciences, Xavier University of Louisiana, 1 Drexel Dr, New Orleans, LA, 70125, USA. FAU - Perkins, Clark AU - Perkins C AD - College of Arts and Sciences, Xavier University of Louisiana, 1 Drexel Dr, New Orleans, LA, 70125, USA. FAU - Schenk-Smith, Emily AU - Schenk-Smith E AD - College of Pharmacy, Xavier University of Louisiana, 1 Drexel Dr, New Orleans, LA, 70125, USA. FAU - Ali, Simak AU - Ali S AD - Department of Surgery and Cancer, Imperial College of London, South Kensington Campus, London, SW7 2AZ, UK. FAU - Burow, Matthew AU - Burow M AD - Department of Medicine, Hematology, and Medical Oncology, Tulane University School of Medicine, 1430 Tulane Ave, New Orleans, LA, 70112, USA. FAU - Williams, Christopher AU - Williams C AD - College of Pharmacy, Xavier University of Louisiana, 1 Drexel Dr, New Orleans, LA, 70125, USA. cwilli35@xula.edu. LA - eng GR - #U54MD007595/NH/NIH HHS/United States GR - #D3400006/HRSA/HRSA HHS/United States GR - Core Grant for the Xavier University of Louisiana/Louisiana Cancer Research Consortium, Xavier University of Louisiana/ PT - Journal Article DEP - 20260802 PL - England TA - Breast Cancer Res JT - Breast cancer research : BCR JID - 100927353 RN - EC 2.7.11.1 (Casein Kinase II) RN - EC 2.7.10.1 (Erb-b2 Receptor Tyrosine Kinases) RN - 0VUA21238F (Lapatinib) RN - EC 2.7.10.1 (ErbB Receptors) RN - 0 (Protein Kinase Inhibitors) RN - 0 (Phenazines) RN - EC 2.7.10.1 (ERBB2 protein, human) RN - C6RWP0N0L2 (silmitasertib) RN - 0 (Antineoplastic Agents) RN - EC 2.7.10.1 (EGFR protein, human) RN - 0 (Quinazolines) RN - 0 (Naphthyridines) SB - IM MH - Humans MH - *Casein Kinase II/antagonists & inhibitors/genetics/metabolism MH - *Erb-b2 Receptor Tyrosine Kinases/antagonists & inhibitors/metabolism MH - Female MH - *Epithelial-Mesenchymal Transition/drug effects MH - *Lapatinib/pharmacology MH - *Breast Neoplasms/drug therapy/pathology/metabolism/genetics MH - ErbB Receptors/antagonists & inhibitors MH - Cell Proliferation/drug effects MH - Cell Line, Tumor MH - *Protein Kinase Inhibitors/pharmacology MH - Phenazines/pharmacology MH - Signal Transduction/drug effects MH - Drug Resistance, Neoplasm MH - *Antineoplastic Agents/pharmacology MH - Animals MH - Quinazolines/pharmacology MH - Gene Expression Regulation, Neoplastic/drug effects MH - Naphthyridines OTO - NOTNLM OT - Breast cancer OT - CK2 OT - CX-4945 OT - EMT OT - ERK signaling OT - ERalpha Y537S OT - Endocrine resistance COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no conflicting interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 00:59 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/03/05 00:00 [received] PHST- 2026/07/24 00:00 [accepted] PHST- 2026/10/30 00:59 [entrez] AID - 10.1186/s13058-026-02356-y [pii] AID - 10.1186/s13058-026-02356-y [doi] PST - epublish SO - Breast Cancer Res. 2026 Aug 2;28(1):184. doi: 10.1186/s13058-026-02356-y. PMID- 42958288 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1479-5876 (Electronic) IS - 1479-5876 (Linking) VI - 24 IP - 1 DP - 2026 Oct 17 TI - MRI-based intratumoral and peritumoral heterogeneity for predicting intracranial progression in EGFR-mutant lung adenocarcinoma patients with treatment-naive brain metastases receiving first-line third-generation EGFR-TKIs. LID - 1361 [pii] LID - 10.1186/s12967-026-09109-7 [doi] AB - PURPOSE: To develop and externally test a multiparametric MRI habitat-based framework integrating intratumoral heterogeneity (ITH), peritumoral heterogeneity (PTH), and clinical characteristics for predicting intracranial progression in patients with EGFR-mutant lung adenocarcinoma and de novo brain metastases receiving first-line third-generation (3rd-gen) EGFR-TKI monotherapy. MATERIALS AND METHODS: This retrospective multicenter study included patients with EGFR-mutant lung adenocarcinoma and de novo brain metastases who received first-line 3rd-gen EGFR-TKI monotherapy. Patients were assigned to an internal training cohort (n = 90) and an external testing cohort (n = 51). The binary prediction outcome was intracranial progression within 20 months after treatment initiation. Pretreatment contrast-enhanced T1-weighted imaging, T2-weighted imaging, and diffusion-weighted imaging were analyzed. The index brain metastasis and a 6-mm peritumoral region were segmented, followed by voxel-wise habitat clustering. Habitat-specific radiomic features were extracted and selected to construct ITH, PTH, and combined intratumoral-peritumoral habitat (PITH) signatures. Clinical predictors were evaluated using univariable and multivariable logistic regression. Support vector machine models were evaluated using receiver operating characteristic, decision curve, calibration, and Kaplan-Meier analyses. RESULTS: A total of 141 patients were analyzed, including 75 with progressive disease (PD) and 66 without PD (non-PD). Multivariable logistic regression identified EGFR mutation subtype (odds ratio [OR] = 2.390, 95% confidence interval [CI]: 1.187-4.811, P = 0.015) and the maximum diameter of the index brain metastasis as independent predictors (OR = 2.048, 95% CI: 1.204-3.483, P = 0.008). Models based on imaging heterogeneity features outperformed the clinical model overall. The clinical model achieved an AUC of 0.662, while the ITH, PTH, and PITH models achieved AUCs of 0.715, 0.724, and 0.758, respectively. The combined model showed favorable performance (AUC 0.795, accuracy 76.5%, sensitivity 70.4%, and specificity 83.3%). Patients classified by the combined model as high risk had significantly shorter intracranial progression-free survival (iPFS), than those classified as low risk (log-rank P = 0.019). CONCLUSION: The proposed framework integrating ITH, PTH, and clinical features may provide a noninvasive approach for predicting intracranial progression risk and stratifying prognosis in patients with EGFR-mutant lung adenocarcinoma with newly diagnosed BMs receiving first-line 3rd-gen EGFR-TKI monotherapy. CI - (c) 2026. The Author(s). FAU - Deng, Guangchuan AU - Deng G AD - Department of Cancer Center, The Second Affiliated Hospital of Chongqing Medical University, Tianwen Avenue No. 288, Nan'an District, Chongqing, 400010, China. FAU - Li, Houying AU - Li H AD - Department of Radiology, The Second Qilu Hospital of Shandong University, Jinan, 250033, China. FAU - Dai, Zhengjun AU - Dai Z AD - Institute of Research and Clinical Innovations, Neusoft Medical Systems Co. Ltd, Beijing, 100094, China. FAU - Fan, Jing AU - Fan J AD - Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan City, 250117, China. FAU - Song, Xiaopeng AU - Song X AD - Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan City, 250117, China. FAU - Pang, Guodong AU - Pang G AD - Department of Radiology, The Second Qilu Hospital of Shandong University, Jinan, 250033, China. FAU - Li, Zhenxiang AU - Li Z AD - Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan City, 250117, China. zxli@email.sdfmu.edu.cn. FAU - Zhao, Fang AU - Zhao F AD - Department of Radiology, Qilu Hospital of Shandong University, Jinan, 250012, China. zhaofang27@126.com. LA - eng GR - Grant No.82272752/National Natural Science Foundation of China/ GR - No. ZF003/Collaborative Academic Innovation Project of Shandong Cancer Hospital/ PT - Journal Article PT - Multicenter Study DEP - 20261017 PL - England TA - J Transl Med JT - Journal of translational medicine JID - 101190741 RN - EC 2.7.10.1 (ErbB Receptors) RN - 0 (Protein Kinase Inhibitors) RN - EC 2.7.10.1 (EGFR protein, human) SB - IM MH - Humans MH - *Brain Neoplasms/secondary/diagnostic imaging/drug therapy/genetics MH - Male MH - ErbB Receptors/genetics MH - Female MH - *Mutation/genetics MH - *Disease Progression MH - *Protein Kinase Inhibitors/therapeutic use/pharmacology MH - *Adenocarcinoma of Lung/drug therapy/genetics/pathology/diagnostic imaging MH - *Magnetic Resonance Imaging MH - Radiomics MH - Aged MH - Middle Aged MH - *Lung Neoplasms/pathology/drug therapy/genetics/diagnostic imaging MH - ROC Curve MH - Retrospective Studies OTO - NOTNLM OT - De novo brain metastases OT - Intratumoral heterogeneity OT - MRI OT - Peritumoral heterogeneity OT - Third-Generation EGFR-TKI monotherapy OT - Tumor habitat COIS- Declarations. Ethics approval and consent to participate: This study was approved by the Ethics Committee of Shandong Cancer Hospital and Institute and was conducted in accordance with the Declaration of Helsinki. Patients were diagnosed and treated at Shandong Cancer Hospital and Institute, The Second Qilu Hospital of Shandong University, and Qilu Hospital of Shandong University. Given the retrospective nature of the study and the policy of the Ethics Committee, the requirement for informed consent was waived. The data used in this study were anonymized before analysis. Competing interests: The authors declare that they have no known competing financial interests or personal relationships. All authors have no conflict of interest with any company or organization. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 00:58 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/05/20 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/30 00:58 [entrez] AID - 10.1186/s12967-026-09109-7 [pii] AID - 10.1186/s12967-026-09109-7 [doi] PST - epublish SO - J Transl Med. 2026 Oct 17;24(1):1361. doi: 10.1186/s12967-026-09109-7. PMID- 42958287 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1472-6920 (Electronic) IS - 1472-6920 (Linking) VI - 26 IP - 1 DP - 2026 Oct 23 TI - General information reliability, perceived educational quality, and transparency of YouTube videos on epileptiform discharges: an exploratory cross-sectional study. LID - 1755 [pii] LID - 10.1186/s12909-026-10648-2 [doi] AB - OBJECTIVE: Electroencephalography (EEG) is central to diagnosing and classifying epileptiform discharges, but interpretation requires structured training. This exploratory cross-sectional study evaluated general information reliability and completeness, perceived educational quality, and transparency of English-language YouTube videos on epileptiform discharges. Although these patterns are described using established electrographic criteria, interpretation may vary, particularly in borderline recordings. METHODS: A cross-sectional YouTube search was conducted in May 2025 using five keywords. The first 100 results per keyword were screened and 25 of 500 records met all eligibility criteria. The final sample was limited to narrated, English-language videos lasting 1 to 20 min that were returned by the selected search terms and YouTube ranking system. Two neurologists independently rated the videos using DISCERN as a general reliability indicator, the Global Quality Scale (GQS) for educational quality, and the Journal of the American Medical Association (JAMA) criteria for transparency. Exploratory analyses included group comparisons, Spearman correlations, single-measure intraclass correlation coefficients (ICCs), effect sizes, confidence intervals, and false-discovery-rate-adjusted q values. RESULTS: Six videos (24%) were from academic/educational channels and 19 (76%) from individual healthcare professionals. Mean scores were 52.1 for DISCERN (95% CI 46.7-57.5), 3.86 for GQS (95% CI 3.58-4.14), and 1.98 for JAMA (95% CI 1.69-2.27). Single-measure ICCs were 0.971, 0.655, and 0.816, respectively. Duration was nominally correlated with GQS (rho = 0.431, p = 0.031) and JAMA (rho = 0.553, p = 0.004), and DISCERN with GQS (rho = 0.496, p = 0.012); none remained significant after false-discovery-rate correction (all q >/= 0.052). CONCLUSION: Among the small subset of eligible videos, general-purpose instruments suggested moderate-to-good average perceived educational quality and information reliability, whereas transparency was limited. Because DISCERN, GQS, and JAMA are not EEG-specific and no qualitative EEG-accuracy, pedagogical, or learner-outcome assessment was performed, the findings are exploratory. These results should not be understood as estimating the quality of all YouTube material related to EEG or epileptiform discharges, nor do they establish EEG-specific accuracy or educational effectiveness. The evaluated videos should therefore be used only as critically appraised adjuncts to expert-supervised EEG training. CI - (c) 2026. The Author(s). FAU - Unal, Elif AU - Unal E AUID- ORCID: 0000-0002-6830-1788 AD - Department of Neurology, Prof. Dr. Cemil Tascioglu City Hospital, Istanbul, Turkiye. elifunalmd@yahoo.com. FAU - Issi, Elif Simin AU - Issi ES AUID- ORCID: 0000-0003-1950-9141 AD - Department of Neurology, Faculty of Medicine, Afyonkarahisar Health Sciences University, Afyonkarahisar , Turkiye. LA - eng PT - Journal Article DEP - 20261023 PL - England TA - BMC Med Educ JT - BMC medical education JID - 101088679 SB - IM MH - Cross-Sectional Studies MH - Humans MH - *Video Recording/standards MH - Reproducibility of Results MH - *Social Media/standards MH - *Epilepsy/diagnosis/physiopathology MH - *Electroencephalography MH - Information Dissemination OTO - NOTNLM OT - EEG OT - Epileptiform discharges OT - Medical education OT - Reliability OT - YouTube COIS- Declarations. Ethics approval and consent to participate: Ethical approval was not required as the study used publicly available data. Consent for publication: Not applicable, as this study did not involve human participants, patient data, or identifiable personal information. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 00:58 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/02/18 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/30 00:58 [entrez] AID - 10.1186/s12909-026-10648-2 [pii] AID - 10.1186/s12909-026-10648-2 [doi] PST - epublish SO - BMC Med Educ. 2026 Oct 23;26(1):1755. doi: 10.1186/s12909-026-10648-2. PMID- 42958276 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 1472-6955 (Print) IS - 1472-6955 (Linking) VI - 25 IP - 1 DP - 2026 Aug 3 TI - Associations of alarm fatigue with occupational burnout and sleep quality in ICU nurses: a cross-sectional study. LID - 1042 [pii] LID - 10.1186/s12912-026-05165-y [doi] AB - BACKGROUND: Alarm fatigue is a critical concern in intensive care units (ICUs), where nurses are continuously exposed to high volumes of clinical alarms. This phenomenon has been associated with psychological strain, impaired sleep quality, and occupational burnout. Despite growing evidence, limited studies have simultaneously explored the associations among these variables in ICU nurses. This study aimed to investigate the relationships among alarm fatigue, occupational burnout, and sleep quality in ICU nurses working in hospitals in Kerman and Sirjan, Iran. METHODS: A descriptive-analytical cross-sectional study was conducted among 219 ICU nurses selected through census sampling. Data were collected using a demographic questionnaire, the Alarm Fatigue Scale (AFSS), the Maslach Burnout Inventory (MBI), and the Pittsburgh Sleep Quality Index (PSQI). Statistical analyses were performed using SPSS version 22, employing independent t-test, ANOVA, Mann-Whitney U, Kruskal-Wallis, Spearman's correlation coefficient, and multivariable linear regression analyses. A significance level of p < 0.05 was considered. RESULTS: More than half of the nurses reported no alarm fatigue (57.5%), while 83.6% experienced poor sleep quality. Emotional exhaustion was low to moderate in most participants, depersonalization was moderate in 56.2%, and reduced personal accomplishment was reported by 96.3%. Alarm fatigue showed weak but statistically significant positive correlations with poor sleep quality (r = 0.230, p = 0.001) and occupational burnout (r = 0.166, p = 0.014). Sleep quality was also weakly but significantly correlated with occupational burnout (r = 0.175, p = 0.010). Alarm fatigue and burnout were significantly associated with gender, employment status, shift type, and general health status. CONCLUSIONS: Alarm fatigue, poor sleep quality, and occupational burnout are related occupational health concerns among ICU nurses. Higher alarm fatigue was weakly but significantly associated with poorer sleep quality and greater burnout. These findings suggest that alarm management, shift scheduling, and workforce-related factors may be relevant areas for organizational attention; however, future longitudinal and interventional studies are needed to determine whether such strategies improve sleep quality, reduce burnout, or enhance patient safety. CLINICAL TRIAL NUMBER: Not applicable. CI - (c) 2026. The Author(s). FAU - Zeidabadinejad, Somayeh AU - Zeidabadinejad S AD - Student Research Committee, Department of Anesthesiology, Sirjan School of Medical Sciences, Sirjan, Iran. FAU - Sadeghi, Reza AU - Sadeghi R AD - Department of Public Health, Sirjan School of Medical Sciences, Sirjan, Iran. FAU - Abolghaseminejad, Parniya AU - Abolghaseminejad P AUID- ORCID: 0000-0002-8114-4692 AD - Department of Health Education and Promotion, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. parniya.ab.20@gmail.com. FAU - Nosratabadi, Iman AU - Nosratabadi I AD - Department of Nursing, Sirjan School of Medical Sciences, Sirjan, Iran. FAU - Lari, Leyla Ahmadi AU - Lari LA AD - Department of Anesthesiology, School of Nursing, Larestan University of Medical Sciences, Larestan, Iran. FAU - Baseri, Fatemeh Yousefi AU - Baseri FY AD - Student Research Committee, Sirjan School of Medical Sciences, Sirjan, Iran. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - BMC Nurs JT - BMC nursing JID - 101088683 OTO - NOTNLM OT - Alarm fatigue OT - ICU nurses OT - Occupational burnout OT - Sleep quality COIS- Declarations. Ethics approval and consent to participate: Ethical approval was obtained from the Ethics Committee of Sirjan School of Medical Sciences (Approval Code: IR.SIRUMS.REC.1404.008). Researchers obtained the necessary permits from the relevant nursing management departments and hospital administrators. The study objectives were explained to the participating nurses, and confidentiality and voluntary participation were emphasized. Accordingly, verbal informed consent was obtained from all individual participants included in the study. All participants were explicitly assured that their participation was voluntary and that they could withdraw from the study at any stage without any negative consequences. All methods were performed in accordance with the relevant guidelines and regulations, including the Declaration of Helsinki. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 00:58 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/05/25 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/30 00:58 [entrez] AID - 10.1186/s12912-026-05165-y [pii] AID - 10.1186/s12912-026-05165-y [doi] PST - epublish SO - BMC Nurs. 2026 Aug 3;25(1):1042. doi: 10.1186/s12912-026-05165-y. PMID- 42958270 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1465-993X (Electronic) IS - 1465-9921 (Linking) VI - 27 IP - 1 DP - 2026 Jul 27 TI - Integrated host-microbe biomarkers for early diagnostic classification of paediatric LRTI in mechanically ventilated children. LID - 425 [pii] LID - 10.1186/s12931-026-03829-6 [doi] AB - BACKGROUND: Early diagnosis of paediatric lower respiratory tract infection (LRTI) in intensive care is difficult because clinical features overlap with non-infectious respiratory failure and conventional microbiology has limited sensitivity. We aimed to integrate airway microbial and host-response signals to identify early diagnostic biomarkers in mechanically ventilated children. METHODS: We re-analysed airway metagenomic sequencing and tracheal aspirate transcriptomics from 261 ventilated paediatric intensive care unit (PICU) patients using differential expression, protein-protein interaction network analysis, and machine-learning feature selection. Findings were evaluated in an exploratory assessment of biologically related cytokine markers within an independent prospective cohort (RASCALS; n = 100 enrolled, n = 78 analysed) using non-bronchoscopic mini-bronchoalveolar lavage (mini-BAL), plasma cytokines, and clinician-adjudicated diagnoses. RESULTS: Respiratory syncytial virus and Haemophilus influenzae were the only pathogens consistently enriched in LRTI. Host transcriptomics showed activation of interferon, cytokine, and chemokine signalling. Network-guided feature selection identified a seven-gene panel (IRF7, FFAR3, GZMB, FABP4, FN1, CXCL5, BCAR1) with high diagnostic performance in cross-validation (median F1 0.94), comparable to a published 14-gene model. In RASCALS, a logistic model using mini-BAL IL-1beta, IL-4, and IL-8 classified bacterial LRTI with 65% accuracy (sensitivity 70%, specificity 63%), while blood IL-6 and TRAIL achieved 82% accuracy (sensitivity 79%, specificity 83%). CONCLUSIONS: Integrating airway microbial profiles with host-response biomarkers supports earlier and more specific LRTI diagnostic classification in ventilated children. The seven-gene panel and cytokine combinations are candidates for rapid PCR- or immunoassay-based bedside tests to inform antimicrobial stewardship. CI - (c) 2026. The Author(s). FAU - Wu, Zongtai AU - Wu Z AD - Milner Therapeutics Institute, University of Cambridge, Cambridge, UK. AD - Department of Public Health and Primary Care, University of Cambridge, Cambridge, UK. FAU - Youssef, Gehad AU - Youssef G AD - Milner Therapeutics Institute, University of Cambridge, Cambridge, UK. gy260@cam.ac.uk. AD - Cambridge Centre for AI in Medicine, University of Cambridge, Cambridge, UK. gy260@cam.ac.uk. AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. gy260@cam.ac.uk. AD - Department of Quantum Information, Institute for Convergence Science Academy, Yonsei University, Seoul, Republic of Korea. gy260@cam.ac.uk. FAU - Kean, Iain R L AU - Kean IRL AD - Department of Paediatrics, University of Cambridge, Cambridge, UK. FAU - Zhang, Zhenguang AU - Zhang Z AD - Department of Paediatrics, University of Cambridge, Cambridge, UK. FAU - Clark, John A AU - Clark JA AD - Department of Paediatrics, University of Cambridge, Cambridge, UK. FAU - Pathan, Nazima AU - Pathan N AD - Department of Paediatrics, University of Cambridge, Cambridge, UK. AD - Paediatric Intensive Care Unit, Addenbrooke's Hospital, Cambridge, UK. FAU - Han, Namshik AU - Han N AD - Milner Therapeutics Institute, University of Cambridge, Cambridge, UK. nh417@cam.ac.uk. AD - Cambridge Centre for AI in Medicine, University of Cambridge, Cambridge, UK. nh417@cam.ac.uk. AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. nh417@cam.ac.uk. AD - Department of Quantum Information, Institute for Convergence Science Academy, Yonsei University, Seoul, Republic of Korea. nh417@cam.ac.uk. AD - Department of Nano Biomedical Engineering (NanoBME), Advanced Science Institute, Yonsei University, Seoul, Republic of Korea. nh417@cam.ac.uk. AD - Center for Nanomedicine, Institute for Basic Science (IBS), Seoul, Republic of Korea. nh417@cam.ac.uk. LA - eng GR - 900240/Addenbrooke's Charitable Trust, Cambridge University Hospitals/ GR - 900240/Addenbrooke's Charitable Trust, Cambridge University Hospitals/ GR - 900240/Addenbrooke's Charitable Trust, Cambridge University Hospitals/ GR - BRC-1215-20014/NIHR Cambridge Biomedical Research Centre/ GR - RG91966/LifeArc, United Kingdom/ GR - GTL24021-000/National Research Council of Science and Technology/ GR - RS-2025-18362970/National Research Foundation of Korea/ GR - RS-2025-25456722/Korea Health Industry Development Institute/ GR - RS-2025-25427881/Brain Pool Plus Fellowship Program, South Korea/ PT - Journal Article DEP - 20260727 PL - England TA - Respir Res JT - Respiratory research JID - 101090633 RN - 0 (Biomarkers) SB - IM MH - Humans MH - *Respiration, Artificial/trends/adverse effects MH - Biomarkers/metabolism MH - *Respiratory Tract Infections/diagnosis/microbiology/classification/genetics MH - Male MH - Female MH - Early Diagnosis MH - Prospective Studies MH - Child, Preschool MH - Infant MH - Child MH - *Host-Pathogen Interactions/physiology MH - Bronchoalveolar Lavage Fluid/microbiology MH - Intensive Care Units, Pediatric/trends OTO - NOTNLM OT - Diagnostic biomarkers OT - Haemophilus influenzae OT - Host transcriptomics OT - Lower respiratory tract infection OT - Metagenomic sequencing OT - Multi-omics integration OT - Paediatric intensive care OT - Respiratory syncytial virus COIS- Declarations. Ethics approval and consent to participate: This study includes secondary analysis of clinical samples and data collected under the Rapid Assay for Sick Children with Acute Lung Infection Study (RASCALS [12]). The original RASCALS study received ethical approval from the Yorkshire and the Humber, Bradford Leeds Research Ethics Committee (Research Ethics Committee reference 20/YH/0089), under the governance of the UK Health Research Authority (HRA). Ethical approval was granted for prospective participant recruitment, sample collection, and the use of routine and research-based diagnostic assays. Deferred consent for up to 48 hours was permitted in accordance with the approved protocol. For the current analysis, all samples and associated clinical data were anonymised prior to inclusion, and therefore no additional ethical approval was required. Informed consent was obtained from parents or legal guardians of all participating children enrolled in the RASCALS study. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 00:57 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2025/12/29 00:00 [received] PHST- 2026/07/13 00:00 [accepted] PHST- 2026/10/30 00:57 [entrez] AID - 10.1186/s12931-026-03829-6 [pii] AID - 10.1186/s12931-026-03829-6 [doi] PST - epublish SO - Respir Res. 2026 Jul 27;27(1):425. doi: 10.1186/s12931-026-03829-6. PMID- 42958268 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1479-5876 (Electronic) IS - 1479-5876 (Linking) VI - 24 IP - 1 DP - 2026 Aug 5 TI - Large-scale compound screening reveals sensitivities to SM1-71, HDACs/mTOR Inhibitor 1, BGT226, and ON013100 in glioma. LID - 1362 [pii] LID - 10.1186/s12967-026-08771-1 [doi] AB - BACKGROUND: Gliomas, particularly glioblastomas (GBM), are the most aggressive primary brain tumors with poor prognosis and limited therapeutic options. Resistance to temozolomide and high tumor invasiveness underscore the urgent need for novel therapeutic agents. METHODS: We performed a large-scale screening of 1,460 small-molecule compounds using three human glioma cell lines (U251, LN229, and A172) to identify candidate anti-glioma agents. Four compounds with limited prior investigation in gliomas-SM1-71 (TAK1 inhibitor), HDACs/mTOR Inhibitor 1, BGT226 (PI3K/mTOR inhibitor), and ON013100 (mitotic inhibitor)-were selected for detailed in vitro and in vivo evaluation. In vitro assays included cell viability (CCK-8), cell cycle analysis, apoptosis, migration, and invasion assays. In vivo efficacy was assessed in a subcutaneous xenograft mouse model. RESULTS: SM1-71, HDACs/mTOR Inhibitor 1, BGT226, and ON013100 significantly inhibited glioma cell proliferation, induced G2-phase cell cycle arrest, and promoted apoptosis. All four compounds suppressed glioma cell migration and invasion in vitro. In the xenograft model, SM1-71 and ON013100 showed the most pronounced tumor growth inhibition (~ 75% reduction), whereas BGT226 and HDACs/mTOR Inhibitor 1 exhibited moderate effects. Ki-67 immunostaining confirmed decreased tumor cell proliferation. No significant body weight loss was observed, although isolated mortality indicated the need for further toxicity evaluation. CONCLUSIONS: This study identifies four promising anti-glioma compounds with potent in vitro and in vivo activity, particularly SM1-71 and ON013100. These findings support further preclinical investigation of these compounds, particularly with respect to pharmacokinetics, blood-brain barrier penetration, toxicity, and combination with standard therapies. CI - (c) 2026. The Author(s). FAU - Zhang, Yahui AU - Zhang Y AD - Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, 266000, China. FAU - Xie, Mingchen AU - Xie M AD - Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, 266000, China. FAU - Han, Hao AU - Han H AD - Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, 266000, China. FAU - Xie, Xun AU - Xie X AD - Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, 266000, China. FAU - Cheng, Jianhua AU - Cheng J AD - Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, 266000, China. cjh19940317@163.com. FAU - Xu, Jian AU - Xu J AD - Department of Neurosurgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong Province, 266000, China. xujian@qdu.edu.cn. LA - eng GR - 82503929/the National Natural Science Foundation of China/ GR - ZR2025QC892/Natural Science Foundation of Shandong Province/ GR - tsqn202408393/Taishan Scholar Foundation of Shandong Province/ GR - 25-1-1-140-zyyd-jch/Natural Science Foundation of Qingdao Municipality/ GR - 202304041486/Shandong Province Medical and Health Science and Technology Project/ PT - Journal Article DEP - 20260805 PL - England TA - J Transl Med JT - Journal of translational medicine JID - 101190741 RN - 0 (Histone Deacetylase Inhibitors) RN - 0 (MTOR Inhibitors) RN - EC 2.7.11.1 (TOR Serine-Threonine Kinases) RN - JGH0DF1U03 (sapanisertib) RN - 96265TNH2R (gedatolisib) RN - 0 (Morpholines) RN - 0 (Pyrimidines) RN - 0 (Benzamides) RN - 0 (Indoles) RN - JAC85A2161 (Adenine) RN - 0 (Benzoxazoles) RN - 0 (Triazines) SB - IM MH - Humans MH - Animals MH - *Glioma/drug therapy/pathology MH - Cell Line, Tumor MH - Apoptosis/drug effects MH - *Histone Deacetylase Inhibitors/pharmacology/therapeutic use MH - Cell Movement/drug effects MH - Cell Proliferation/drug effects MH - *MTOR Inhibitors/pharmacology/therapeutic use MH - Xenograft Model Antitumor Assays MH - *Brain Neoplasms/drug therapy/pathology MH - Mice, Nude MH - *TOR Serine-Threonine Kinases/antagonists & inhibitors/metabolism MH - Drug Screening Assays, Antitumor MH - *Morpholines/pharmacology/therapeutic use MH - *Pyrimidines/pharmacology/therapeutic use MH - *Benzamides/pharmacology/therapeutic use MH - Neoplasm Invasiveness MH - Mice MH - Indoles/pharmacology MH - Adenine/analogs & derivatives MH - Benzoxazoles MH - Triazines OTO - NOTNLM OT - Antitumor activity OT - BGT226 OT - Compound screening OT - Glioblastoma OT - Glioma OT - HDACs/mTOR inhibitor 1 OT - ON013100 OT - SM1-71 COIS- Declarations. Conflict of interest: The authors declare that they have no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 00:57 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/05/11 00:00 [received] PHST- 2026/07/31 00:00 [accepted] PHST- 2026/10/30 00:57 [entrez] AID - 10.1186/s12967-026-08771-1 [pii] AID - 10.1186/s12967-026-08771-1 [doi] PST - epublish SO - J Transl Med. 2026 Aug 5;24(1):1362. doi: 10.1186/s12967-026-08771-1. PMID- 42958262 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1476-7120 (Electronic) IS - 1476-7120 (Linking) VI - 24 IP - 1 DP - 2026 Oct 28 TI - Echocardiographic markers for left atrial stiffness and left atrial conduction delay in patients with silent paroxysmal atrial fibrillation compared to age-matched controls. LID - 32 [pii] LID - 10.1186/s12947-026-00395-1 [doi] AB - PURPOSE: To study left atrial stiffness index (LASI) and left atrial activation time (LA-AT) changes in an older population with silent paroxysmal atrial fibrillation (PAF) compared to age-matched control. METHODS: Individuals with newly diagnosed PAF and a randomly selected age-matched control group without AF were enrolled from STROKESTOP2-study. All participants had NT-proBNP levels 125-900 ng/L. TTE examinations were analyzed retrospectively offline using dedicated software. Besides conventional echocardiographic measurements, LA-AT was obtained by measuring the time delay between the onset of the P-wave on ECG and tissue Doppler A peak wave in lateral LA wall. LASI was calculated as the ratio of left ventricular filling (E/e) to peak atrial longitudinal strain (PALS). RESULTS: Mean age was 76 +/- 0.3 years. The PAF group (n = 67) presented significant increase of LASI (0.41 (0.29-0.60) vs. 0.32 (0.29-0.43), P = 0.001) and prolonged LA-AT (170 (140-180) ms vs. 140 (120-144) ms, P < 0.001) compared to controls (n = 94). Subgroup analysis in participants with normal LA volume index (LAVI) 0.54 and LA-AT > 139 msec for discrimination of silent PAF. CONCLUSION: Indices reflecting LA stiffness and atrial conduction delay were significantly elevated in recently diagnosed PAF patients. LASI and LA-AT thresholds might non-invasively detect early atrial remodeling without structural enlargement in elderly patients with high risk of PAF and mildly increased NT-proBNP levels. CI - (c) 2026. The Author(s). FAU - Bastos, Larissa AU - Bastos L AUID- ORCID: 0000-0001-7767-4159 AD - Department of Clinical Sciences, Danderyds Hospital, Karolinska Institutet, Entrevagen 2, Stockholm, SE-182 88, Sweden. larissa.bastos@ki.se. AD - Department Clinical Physiology, Danderyd Hospital, Stockholm, SE-182 88, Sweden. larissa.bastos@ki.se. FAU - Al-Khalili, Faris AU - Al-Khalili F AUID- ORCID: 0000-0002-8849-7052 AD - Department of Clinical Sciences, Danderyds Hospital, Karolinska Institutet, Entrevagen 2, Stockholm, SE-182 88, Sweden. FAU - Back, Magnus AU - Back M AUID- ORCID: 0000-0003-0853-5141 AD - Department of Medicine, Karolinska Institutet, Stockholm, SE-171 77, Sweden. AD - Department of Cardiology, Karolinska University Hospital, Stockholm, SE-171 76, Sweden. FAU - Manouras, Aristomenis AU - Manouras A AUID- ORCID: 0000-0002-6617-2070 AD - Department of Medicine, Karolinska Institutet, Stockholm, SE-171 77, Sweden. AD - Department of Cardiology, Karolinska University Hospital, Stockholm, SE-171 76, Sweden. FAU - Engdahl, Johan AU - Engdahl J AUID- ORCID: 0000-0002-1677-7215 AD - Department of Clinical Sciences, Danderyds Hospital, Karolinska Institutet, Entrevagen 2, Stockholm, SE-182 88, Sweden. AD - Department of Cardiology, Danderyd Hospital, Stockholm, SE-182 88, Sweden. FAU - Shahgaldi, Kambiz AU - Shahgaldi K AUID- ORCID: 0000-0001-9677-8252 AD - Department of Clinical Sciences, Danderyds Hospital, Karolinska Institutet, Entrevagen 2, Stockholm, SE-182 88, Sweden. AD - Department Clinical Physiology, Danderyd Hospital, Stockholm, SE-182 88, Sweden. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Cardiovasc Ultrasound JT - Cardiovascular ultrasound JID - 101159952 SB - IM MH - Humans MH - *Atrial Fibrillation/physiopathology/diagnosis MH - Female MH - *Atrial Function, Left/physiology MH - Male MH - Aged MH - *Heart Atria/physiopathology/diagnostic imaging MH - Retrospective Studies MH - *Heart Conduction System/physiopathology/diagnostic imaging MH - *Echocardiography, Doppler/methods MH - Electrocardiography OTO - NOTNLM OT - Left atrial activation time OT - Left atrial stiffness index OT - Paroxysmal atrial fibrillation OT - Peak atrial longitudinal strain OT - Tissue Doppler COIS- Declarations. Ethics approval: This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by regional Ethics Committee of Stockholm (DNR 2015/2079-31/3) (DNR 2017/1322-32). Consent to participate: Written and informed consent was obtained from all individual participants included in the study. Competing interests: Kambiz Shahgaldi has received lecture fees from General Electric Healthcare.The rest of the authors have no relevant financial or non-financial interest to disclose. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 00:57 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2025/10/19 00:00 [received] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/30 00:57 [entrez] AID - 10.1186/s12947-026-00395-1 [pii] AID - 10.1186/s12947-026-00395-1 [doi] PST - epublish SO - Cardiovasc Ultrasound. 2026 Oct 28;24(1):32. doi: 10.1186/s12947-026-00395-1. PMID- 42958261 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2490 (Electronic) IS - 1471-2490 (Linking) VI - 26 IP - 1 DP - 2026 Aug 4 TI - Exploratory development and internal validation of sex-specific nomograms for clinically documented short-term urinary retention during post-biopsy immobilization after percutaneous renal biopsy. LID - 271 [pii] LID - 10.1186/s12894-026-02296-x [doi] AB - OBJECTIVE: To develop and internally evaluate exploratory sex-specific models for clinically documented short-term urinary retention (UR) during mandatory post-biopsy immobilization after percutaneous renal biopsy (PRB). METHODS: A total of 566 adults who underwent PRB at a tertiary hospital in Shanghai between January 2023 and November 2024 were screened. The retrospective outcome was clinically documented UR requiring catheterization and meeting a record-based volume or time criterion. Routine pre-catheterization bladder scanning was not available for all patients. Univariate analyses were descriptive; each sex-specific logistic model was restricted to four clinically selected, routinely available pre-biopsy predictors and was internally evaluated using five-fold cross-validation and bootstrap calibration. RESULTS: Ten patients with oliguria were excluded, leaving 556 for analysis; clinically documented UR was recorded in 78 patients (14.03%). The four-variable male model included age > 60 years, benign prostatic hyperplasia (BPH), diabetes mellitus (DM), and preoperative voiding training; BPH, DM, and absence of training were statistically significant. The four-variable female model included hypertension, history of UR, preoperative voiding training, and bowel movement within 24 h before PRB; hypertension, absence of training, and no bowel movement were statistically significant. Cross-validated AUCs were 0.793 for males and 0.748 for females. CONCLUSION: These nomograms are hypothesis-generating models of a clinically documented care-pathway outcome. Their moderate internal discrimination does not overcome the limitations of retrospective outcome ascertainment or the low event-to-predictor ratio in the male cohort. Standardized objective outcome measurement and external validation are required before clinical use. TRIAL REGISTRATION: Not applicable. CI - (c) 2026. The Author(s). FAU - Sang, Xiuping AU - Sang X AD - Department of Nephrology, The First Affiliated Hospital of Naval Medical University, Shanghai, China. FAU - Du, Jun AU - Du J AD - Department of Nephrology, The First Affiliated Hospital of Naval Medical University, Shanghai, China. FAU - Zhao, Lifang AU - Zhao L AD - Department of Nephrology, The First Affiliated Hospital of Naval Medical University, Shanghai, China. FAU - Wang, Tieyun AU - Wang T AD - Department of Nephrology, The First Affiliated Hospital of Naval Medical University, Shanghai, China. 13917774121@163.com. FAU - Wang, Haiyan AU - Wang H AD - Department of Nephrology, The First Affiliated Hospital of Naval Medical University, Shanghai, China. whaiyansh@163.com. LA - eng GR - (grant numbers 0102018005)/2024 Guangdong Province Famous Traditional Chinese Medicine Xu Youjia Heritage Studio Project/ PT - Journal Article PT - Validation Study DEP - 20260804 PL - England TA - BMC Urol JT - BMC urology JID - 100968571 SB - IM MH - Humans MH - *Urinary Retention/etiology/diagnosis MH - Female MH - *Nomograms MH - Male MH - Retrospective Studies MH - Middle Aged MH - *Kidney/pathology MH - Aged MH - Sex Factors MH - Biopsy MH - Time Factors OTO - NOTNLM OT - Clinically documented urinary retention OT - Internal validation OT - Nomogram OT - Percutaneous renal biopsy OT - Post-biopsy immobilization OT - Retrospective study COIS- Declarations. Ethics approval and consent to participate: The study was conducted in accordance with the Declaration of Helsinki and was approved by the Ethics Committee of The First Affiliated Hospital of Naval Medical University, Shanghai, China (approval number: CHEC2020-171). Because this was a retrospective analysis of existing clinical records, the Ethics Committee waived the requirement for individual informed consent. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 00:57 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/06/15 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/30 00:57 [entrez] AID - 10.1186/s12894-026-02296-x [pii] AID - 10.1186/s12894-026-02296-x [doi] PST - epublish SO - BMC Urol. 2026 Aug 4;26(1):271. doi: 10.1186/s12894-026-02296-x. PMID- 42958258 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1477-3155 (Electronic) IS - 1477-3155 (Linking) VI - 24 IP - 1 DP - 2026 Aug 1 TI - Synergistic promotion of white-to-beige adipocyte transition by manganese-nucleic acid framework and allicin for alleviating obesity. LID - 1004 [pii] LID - 10.1186/s12951-026-04857-6 [doi] AB - Long-term obesity treatment remains challenging due to high costs, adverse drug effects, and invasive surgical procedures. Here, we develop an adipocyte-targeted manganese-nucleic acid framework (MNF) with superoxide dismutase (SOD)-like activity. The MNF and allicin are co-encapsulated into a silk fibroin-sulfobutylether-beta-cyclodextrin (SFSC) microneedle patch, enabling minimally invasive transdermal delivery specifically to subcutaneous adipose tissue. The combination of MNF and allicin exhibits synergistic effects in alleviating oxidative stress, reducing lipid accumulation, and suppressing M1 macrophage polarization and inflammation. Notably, treatment with MNF was observed to increase intracellular NAD(+) levels, while allicin upregulates the SIRT1 signaling pathway. This cooperative action appears to engage the NAD(+)-SIRT1 axis, promoting a phenotypic shift of white adipocytes towards beige adipocytes. Collectively, this microneedle-based delivery system, which promotes a beige-like adipocyte phenotype through dual modulation, presents a promising strategy for long-term obesity management. CI - (c) 2026. The Author(s). FAU - Wang, Teng AU - Wang T AD - Key Laboratory of Precision Nutrition and Food Quality, Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China. FAU - Huangfu, Bingxin AU - Huangfu B AD - Key Laboratory of Precision Nutrition and Food Quality, Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China. FAU - He, Shurong AU - He S AD - Key Laboratory of Precision Nutrition and Food Quality, Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China. FAU - Han, Fengyi AU - Han F AD - Key Laboratory of Precision Nutrition and Food Quality, Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China. FAU - Gao, Jiayi AU - Gao J AD - Key Laboratory of Precision Nutrition and Food Quality, Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China. FAU - Chen, Jinru AU - Chen J AD - Key Laboratory of Precision Nutrition and Food Quality, Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China. FAU - Xu, Wentao AU - Xu W AD - Key Laboratory of Precision Nutrition and Food Quality, Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China. AD - Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), The Ministry of Agriculture and Rural Affairs of the P.R. China, Beijing, 100083, China. FAU - Huang, Kunlun AU - Huang K AD - Key Laboratory of Precision Nutrition and Food Quality, Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China. AD - Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), The Ministry of Agriculture and Rural Affairs of the P.R. China, Beijing, 100083, China. FAU - He, Xiaoyun AU - He X AD - Key Laboratory of Precision Nutrition and Food Quality, Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China. 201211@cau.edu.cn. AD - Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), The Ministry of Agriculture and Rural Affairs of the P.R. China, Beijing, 100083, China. 201211@cau.edu.cn. LA - eng PT - Journal Article DEP - 20260801 PL - England TA - J Nanobiotechnology JT - Journal of nanobiotechnology JID - 101152208 RN - 0 (Sulfinic Acids) RN - 0 (Disulfides) RN - 3C39BY17Y6 (allicin) RN - 42Z2K6ZL8P (Manganese) RN - 0 (Nucleic Acids) SB - IM MH - Animals MH - *Sulfinic Acids/pharmacology/chemistry MH - Disulfides MH - *Obesity/drug therapy/metabolism MH - *Manganese/chemistry/pharmacology MH - Microneedle Drug Delivery MH - *Adipocytes, Beige/drug effects/metabolism/cytology MH - Mice MH - *Adipocytes, White/drug effects/metabolism/cytology MH - Oxidative Stress/drug effects MH - *Nucleic Acids/chemistry/pharmacology MH - Mice, Inbred C57BL MH - Male MH - 3T3-L1 Cells OTO - NOTNLM OT - Allicin OT - Aptamer OT - Manganese-nucleic acid framework OT - Microneedle OT - Obesity COIS- Declarations. Ethics approval and consent to participate: All animal experiments were approved by the Animal Ethics Committee of China Agricultural University (AW42205202-4-2-4-1). Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 00:57 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/05/05 00:00 [received] PHST- 2026/07/21 00:00 [accepted] PHST- 2026/10/30 00:57 [entrez] AID - 10.1186/s12951-026-04857-6 [pii] AID - 10.1186/s12951-026-04857-6 [doi] PST - epublish SO - J Nanobiotechnology. 2026 Aug 1;24(1):1004. doi: 10.1186/s12951-026-04857-6. PMID- 42958255 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1472-6831 (Electronic) IS - 1472-6831 (Linking) VI - 26 IP - 1 DP - 2026 Aug 1 TI - Communication and teamwork between dental surgeons and dental technologists during fabrication of removable complete dentures in Uganda: a qualitative interview study. LID - 2139 [pii] LID - 10.1186/s12903-026-09487-w [doi] AB - BACKGROUND: Edentulous patients seek services from dental professionals to replace lost teeth with removable complete dentures. The dentist and dental technologist are expected to work together to fulfill the patients' expectations. Effective communication is important in ensuring that dentures are well-fabricated and fitted, and also influences the working relationship between the two professionals. This study explored how dental surgeons and dental technologists communicate and work together during the fabrication of removable complete dentures. METHODS: A qualitative study was conducted with dental surgeons and dental technologists at Makerere University Dental Hospital. Semi-structured interviews were conducted with twenty-five purposively selected participants. Data were analyzed using an inductive thematic analysis approach, and NVivo 14 software was used to manage the data. RESULTS: Participants identified effective communication as essential to complete denture fabrication, noting that clear instructions to dental technologists reduce errors. They reported positive relationships with colleagues, characterized by open discussions, mutual support, collaborative problem-solving and willingness to repeat procedures if mistakes occurred. Strong collaboration was viewed as a vital safety measure that helps reduce workload-related errors. Written laboratory request forms were the primary communication tool, supported by phone calls and digital platforms for clarification. Communication challenges included unclear, incomplete or delayed instructions and misinterpretation of instructions. Participants recommended clearer written information, active listening, seeking clarification, using standardized laboratory request forms, timely follow-up, and the use of multiple communication methods. CONCLUSION: Overall, the findings indicate that effective communication and strong collaboration are central to successful complete denture fabrication. Use of multiple complementary communication channels supports the minimization of errors and quality patient care. CI - (c) 2026. The Author(s). FAU - Nono, David AU - Nono D AUID- ORCID: 0009-0007-8443-7582 AD - Department of Clinical Research, Central University of Nicaragua, Managua, Nicaragua. nndvd45@gmail.com. AD - Department of Clinical Research, Texila American University, Georgetown, Guyana. nndvd45@gmail.com. AD - Department of Dental Technology, Makerere University, Kampala, Uganda. nndvd45@gmail.com. AD - Department of Research, Uganda Institute of Allied Health and Management Sciences-Mulago, Kampala, Uganda. nndvd45@gmail.com. FAU - Bagenda, Godfrey AU - Bagenda G AD - Department of Anatomy, Makerere University, Kampala, Uganda. FAU - Okullo, Isaac AU - Okullo I AD - Department of Dental Technology, Makerere University, Kampala, Uganda. FAU - Rwenyonyi, Charles Mugisha AU - Rwenyonyi CM AD - Department of Dental Technology, Makerere University, Kampala, Uganda. LA - eng PT - Journal Article DEP - 20260801 PL - England TA - BMC Oral Health JT - BMC oral health JID - 101088684 SB - IM MH - Humans MH - *Denture, Complete MH - *Dental Technicians/psychology MH - *Communication MH - *Dentists MH - Qualitative Research MH - *Denture Design MH - Dental Care Team MH - *Interprofessional Relations MH - Female MH - Interviews as Topic MH - Male OTO - NOTNLM OT - Communication OT - Dental surgeons OT - Dental technologists OT - Edentulous patients OT - Removable complete dentures OT - Teamwork COIS- Declarations. Ethics approval and consent to participate: Ethical approval for this study was obtained from the Makerere University School of Health Sciences Research and Ethics Committee (MAKSHSREC-2023-486) and the Uganda National Council for Science and Technology (HS3092ES). Administrative clearance was by the Administration of Makerere University Dental Hospital. All participants provide written informed consent before taking part in the study in accordance with the Helsinki Declaration [24]. All collected data were securely stored in a locked cabinet, only accessible to the investigator. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 00:56 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2025/10/24 00:00 [received] PHST- 2026/07/29 00:00 [accepted] PHST- 2026/10/30 00:56 [entrez] AID - 10.1186/s12903-026-09487-w [pii] AID - 10.1186/s12903-026-09487-w [doi] PST - epublish SO - BMC Oral Health. 2026 Aug 1;26(1):2139. doi: 10.1186/s12903-026-09487-w. PMID- 42958254 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1475-2840 (Electronic) IS - 1475-2840 (Linking) VI - 25 IP - 1 DP - 2026 Aug 1 TI - Lipids and apolipoproteins affect the risk of ST-elevation and non-ST-elevation myocardial infarctions differently in type 1 diabetes: a nationwide finnish cohort study. LID - 315 [pii] LID - 10.1186/s12933-026-03315-9 [doi] AB - BACKGROUND: Non-ST-elevation myocardial infarction (NSTEMI) has become the main myocardial infarction subtype in type 1 diabetes compared to ST-elevation myocardial infarction (STEMI), accounting for nearly 80% of myocardial infarction-related hospitalizations. Yet, prior studies in type 1 diabetes have assessed myocardial infarctions as a single entity, potentially overlooking clinically relevant heterogeneity between subtypes in risk assessment. We aimed to study the role of lipids, apolipoproteins, insulin resistance, and the metabolic syndrome in risk stratification of all myocardial infarctions, STEMI and NSTEMI in individuals with type 1 diabetes stratified by the presence of microvascular complications. METHODS: The current study is an observational follow-up study of 4215 individuals from the Finnish Diabetic Nephropathy Study. We verified 449 first-ever myocardial infarctions from medical records and death certificates and further categorized them into either STEMI or NSTEMI. Multivariable Cox regression models were used to assess the effect of a comprehensive lipid and apolipoprotein panel and insulin resistance on the risk of all myocardial infarctions, STEMI, and NSTEMI. Risk estimates for all myocardial infarctions were additionally stratified based on the presence of microvascular complications. RESULTS: For STEMI, apolipoprotein A-I (ApoA-I) (hazard ratio per 1 standard deviation 0.77 [95% confidence interval 0.60-0.99]; P = 0.043), HDL2 cholesterol (HDL2-C) (0.72 [0.55-0.95]; P = 0.019), and HDL-C/total cholesterol ratio (0.76 [0.59-0.98]; P = 0.035) were associated with reduced risk, while no variables were associated with increased risk. Regarding NSTEMI, apolipoprotein B-100 (ApoB-100) (1.31 [1.15-1.50]; P < 0.001) and ApoB-100 to apolipoprotein A-I (ApoA-I) ratio (1.29 [1.18-1.41]; P < 0.001) showed the strongest association with an increased risk, while the HDL-C/total cholesterol ratio was associated with reduced risk (0.71 [0.62-0.82]; P < 0.001). In stratified analyses, associations between selected lipid and apolipoprotein variables and myocardial infarction risk appeared stronger among individuals without microvascular complications. Higher estimated glucose disposal rate, indicating lesser insulin resistance, was associated with reduced risk of both STEMI (0.82 [0.73-0.92]; P < 0.001) and NSTEMI (0.92 [0.86-0.99]; P = 0.024). CONCLUSIONS: We demonstrated that the risk profile between STEMI and NSTEMI differs regarding lipids and apolipoproteins, while insulin resistance was associated with increased risk of both MI subtypes. CI - (c) 2026. The Author(s). FAU - Smidtslund, Patrik AU - Smidtslund P AUID- ORCID: 0000-0001-5844-9925 AD - Folkhalsan Research Center, Helsinki, Finland. AD - Department of Nephrology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. AD - Research Program in Clinical and Molecular Metabolism, University of Helsinki, Helsinki, Finland. FAU - Jansson Sigfrids, Fanny AU - Jansson Sigfrids F AUID- ORCID: 0000-0002-1192-348X AD - Folkhalsan Research Center, Helsinki, Finland. AD - Department of Nephrology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. AD - Research Program in Clinical and Molecular Metabolism, University of Helsinki, Helsinki, Finland. FAU - Harjutsalo, Valma AU - Harjutsalo V AUID- ORCID: 0000-0003-3568-9779 AD - Folkhalsan Research Center, Helsinki, Finland. AD - Department of Nephrology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. AD - Research Program in Clinical and Molecular Metabolism, University of Helsinki, Helsinki, Finland. FAU - Thorn, Ville AU - Thorn V AD - Department of Cardiology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. FAU - Taskinen, Marja-Riitta AU - Taskinen MR AD - Research Program in Clinical and Molecular Metabolism, University of Helsinki, Helsinki, Finland. AD - Department of Cardiology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. FAU - Groop, Per-Henrik AU - Groop PH AUID- ORCID: 0000-0003-4055-6954 AD - Folkhalsan Research Center, Helsinki, Finland. AD - Department of Nephrology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. AD - Research Program in Clinical and Molecular Metabolism, University of Helsinki, Helsinki, Finland. AD - Department of Diabetes, Central Clinical School, Monash University, Melbourne, VIC, Australia. AD - Baker Heart and Diabetes Institute, Melbourne, VIC, Australia. FAU - Thorn, Lena M AU - Thorn LM AUID- ORCID: 0000-0003-3999-0390 AD - Folkhalsan Research Center, Helsinki, Finland. lena.thorn@helsinki.fi. AD - Department of Nephrology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. lena.thorn@helsinki.fi. AD - Research Program in Clinical and Molecular Metabolism, University of Helsinki, Helsinki, Finland. lena.thorn@helsinki.fi. AD - Department of General Practice and Primary Health Care, University of Helsinki, Helsinki University Hospital, P.O. Box 20, Helsinki, FIN-00014, Finland. lena.thorn@helsinki.fi. CN - FinnDiane Study Group LA - eng PT - Comparative Study PT - Journal Article PT - Observational Study DEP - 20260801 PL - England TA - Cardiovasc Diabetol JT - Cardiovascular diabetology JID - 101147637 RN - 0 (Biomarkers) RN - 0 (Lipids) RN - 0 (Apolipoprotein A-I) RN - 0 (Apolipoproteins) SB - IM MH - Humans MH - Female MH - Male MH - Finland/epidemiology MH - *Diabetes Mellitus, Type 1/blood/diagnosis/epidemiology MH - *ST Elevation Myocardial Infarction/epidemiology/blood/diagnosis MH - Middle Aged MH - Risk Factors MH - *Non-ST Elevated Myocardial Infarction/epidemiology/blood/diagnosis MH - Biomarkers/blood MH - Insulin Resistance MH - Adult MH - *Lipids/blood MH - Risk Assessment MH - Apolipoprotein A-I/blood MH - Aged MH - Time Factors MH - *Apolipoproteins/blood MH - Prognosis MH - Metabolic Syndrome/epidemiology/blood/diagnosis MH - *Dyslipidemias/blood/diagnosis/epidemiology OTO - NOTNLM OT - Apolipoproteins OT - Cardiovascular disease OT - Diabetic kidney disease OT - Insulin resistance OT - Lipids OT - Myocardial infarction OT - Non-ST-elevation myocardial infarction OT - ST-elevation myocardial infarction OT - Type 1 diabetes COIS- Declarations. Ethics approval and consent to participate: The FinnDiane Study is approved by the Ethics Committee of HUS Helsinki University Hospital (491/E5/2006, 238/13/03/00/2015, and HUS-3313-2018), and the study is conducted in accordance with the Declaration of Helsinki. Written and informed consent was provided by each participant. Consent for publication: Not applicable. Competing interests: FJS discloses receiving lecture fees from AstraZeneca and Boehringer Ingelheim. VT has received travel support from Medtronic. P-HG has received investigator-initiated research grants from Eli Lilly and Roche, is an advisory board member for AbbVie, Astellas, AstraZeneca, Bayer, Boehringer Ingelheim, Cebix, Eli Lilly, Janssen, Medscape, Merck Sharp & Dohme, Mundipharma, Nestle, Novartis, Novo Nordisk, and Sanofi; and has received lecture fees from Astellas, AstraZeneca, Bayer, Berlin Chemie, Boehringer Ingelheim, Eli Lilly, Elo Water, Genzyme, Merck Sharp & Dohme, Medscape, Menarini, Mundipharma, Novartis, Novo Nordisk, PeerVoice, Sanofi, and Sciarc. All other authors report no conflicts of interest relevant to this article. EDAT- 2026/10/30 05:31 MHDA- 2026/10/30 05:32 CRDT- 2026/10/30 00:56 PHST- 2026/10/30 05:32 [medline] PHST- 2026/10/30 05:31 [pubmed] PHST- 2026/05/18 00:00 [received] PHST- 2026/07/22 00:00 [accepted] PHST- 2026/10/30 00:56 [entrez] AID - 10.1186/s12933-026-03315-9 [pii] AID - 10.1186/s12933-026-03315-9 [doi] PST - epublish SO - Cardiovasc Diabetol. 2026 Aug 1;25(1):315. doi: 10.1186/s12933-026-03315-9. PMID- 42958250 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1755-8794 (Electronic) IS - 1755-8794 (Linking) VI - 19 IP - 1 DP - 2026 Aug 1 TI - SLCO1B3 is associated with poor survival and promotes bladder cancer progression through Wnt/beta-catenin signaling. LID - 165 [pii] LID - 10.1186/s12920-026-02438-y [doi] AB - BACKGROUND: Bladder cancer possesses the highest incidence rates globally. Integrative bioinformatics analyses are necessary to develop predictive biomarkers and potential therapeutic targets. Absorption, distribution, metabolism, and excretion (ADME), the disposition of pharmaceutical compounds in organisms, exert a crucial effect on tumorigenesis. METHODS AND RESULTS: Herein, the Least Absolute Shrinkage and Selection Operator (LASSO) algorithm identified a 6-ADME gene signature comprising CYP1A1, CYP3A5, SLC15A2, SLCO1B3, TPMT, and UGT1A1. Lower risk scores were significantly linked to higher disease-specific survival (DSS)/overall survival (OS) in patients with bladder cancer. The receiver operating characteristic (ROC) curve demonstrated that the risk score-based model exhibited a limited to moderate predictive performance in discriminating bladder cancer patients' outcomes. Univariate and multivariate Cox risk regression analyses identified risk factors for bladder cancer patients' prognosis: risk scores and grade. The nomogram model revealed that its prognostic outcome is reliable. In other words, the risk model could stratify patients with bladder cancer by different clinical characteristics. Moreover, among these 6 ADME key genes, SLCO1B3 was strongly associated with disease-free survival in bladder cancer patients according to GSE32894; however, its specific roles in bladder cancer have not yet been fully elucidated. In vitro, SLCO1B3 knockdown markedly inhibited cell proliferation, migration, and invasion in bladder cancer by regulating the Wnt/beta-catenin signaling axis, which may correlate with its regulation of epithelial-mesenchymal transition (EMT)-related proteins. CONCLUSIONS: A risk model based on 6 key ADME genes has been established using the LASSO algorithm and shows good prognostic potential for patients with bladder cancer. The risk score can also significantly distinguish bladder cancer samples across different clinical statuses, suggesting that the risk model may have diagnostic value for bladder cancer patients. CI - (c) 2026. The Author(s). FAU - Xu, Mingzhe AU - Xu M AD - Department of Urology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China. FAU - Song, Xiaohang AU - Song X AD - Department of Urology, Turpan People's Hospital, Turpan, Xinjiang, 838000, China. FAU - Kudusi AU - Kudusi AD - Department of Urology, Turpan People's Hospital, Turpan, Xinjiang, 838000, China. FAU - Zu, Xiongbing AU - Zu X AD - Department of Urology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China. FAU - Chen, Minfeng AU - Chen M AD - Department of Urology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China. FAU - He, Wei AU - He W AD - Department of Urology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China. xy_heweidoctor@163.com. FAU - Qi, Lin AU - Qi L AD - Department of Urology, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, China. LA - eng GR - 2019JJ50977/Natural Science Foundation of Hunan Province/ GR - 81802561/National Natural Science Foundation of China/ PT - Journal Article DEP - 20260801 PL - England TA - BMC Med Genomics JT - BMC medical genomics JID - 101319628 RN - 0 (Solute Carrier Organic Anion Transporter Family Member 1B3) RN - 0 (SLCO1B3 protein, human) SB - IM MH - Humans MH - *Urinary Bladder Neoplasms/pathology/genetics/metabolism/mortality MH - *Solute Carrier Organic Anion Transporter Family Member 1B3/genetics/metabolism MH - *Wnt Signaling Pathway MH - Prognosis MH - *Disease Progression MH - Gene Expression Regulation, Neoplastic MH - Female MH - Male MH - Cell Line, Tumor OTO - NOTNLM OT - SLCO1B3 OT - Absorption, distribution, metabolism, and excretion (ADME) OT - Bladder cancer OT - Gene signature OT - LASSO OT - Prognosis COIS- Declarations. Ethics approval and consent to participate: This study involving human participants was approved by the Ethics Committee of Xiangya Hospital, Central South University, in accordance with the Declaration of Helsinki (latest revision). Written informed consent was obtained from all participants before inclusion in the study. All procedures were conducted in compliance with relevant institutional, national, and international guidelines and regulations. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:56 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/06 00:00 [received] PHST- 2026/07/22 00:00 [accepted] PHST- 2026/10/30 00:56 [entrez] AID - 10.1186/s12920-026-02438-y [pii] AID - 10.1186/s12920-026-02438-y [doi] PST - epublish SO - BMC Med Genomics. 2026 Aug 1;19(1):165. doi: 10.1186/s12920-026-02438-y. PMID- 42958242 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1476-4598 (Electronic) IS - 1476-4598 (Linking) VI - 25 IP - 1 DP - 2026 Aug 1 TI - Alternative polyadenylation in cancer: mechanisms, functions, and therapeutic potential. LID - 260 [pii] LID - 10.1186/s12943-026-02760-0 [doi] AB - Alternative polyadenylation (APA) is a pervasive post-transcriptional regulatory mechanism that generates transcript isoforms with distinct 3' untranslated regions, terminal exons, or coding potential, thereby influencing mRNA stability, translation, localization, and protein output. Rather than representing a uniform 3'UTR-shortening process, APA comprises heterogeneous 3'-end regulatory events, including tandem 3'UTR APA and upstream APA events such as intronic polyadenylation (IPA), which differ in mechanisms, detection strategies, functional consequences, and clinical interpretation. Dysregulated APA is increasingly recognized as an important contributor to cancer initiation and progression. Tandem 3'UTR APA can remove miRNA- or RNA-binding protein-mediated repression and enhance oncogenic transcript output, whereas upstream APA events, including IPA, can generate prematurely terminated RNAs, truncated proteins, soluble isoforms, or neoantigenic products. In this review, we summarize the classification and biological consequences of major APA events, as well as experimental and computational approaches for APA analysis. We then discuss regulatory mechanisms involving core 3'-end processing factors, RNA-binding proteins, splicing-polyadenylation coupling in upstream APA/IPA, transcription-associated regulation, and promoter-terminator communication. Functionally, aberrant APA contributes to proliferation, invasion and metastasis, metabolic reprogramming, tumor microenvironment remodeling, antitumor immunity, and immune escape. Finally, we highlight APA events, APA regulators, 3'UTR-based signatures, and upstream APA/IPA-derived products as emerging biomarkers and discuss small-molecule, RNA-based, and CRISPR-based strategies for targeting APA or 3'-end processing in cancer. CI - (c) 2026. The Author(s). FAU - Huang, Yu AU - Huang Y AD - Hunan Key Laboratory of Tumor Models and Individualized Medicine, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China. AD - Hunan Engineering Research Center of AI Medical Equipment, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China. AD - Department of Thoracic Surgery, the Second Xiangya Hospital, Central South University, Changsha, Hunan, China. FAU - Li, Zhihong AU - Li Z AD - Hunan Key Laboratory of Tumor Models and Individualized Medicine, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China. AD - Department of Orthopaedics, The Second Xiangya Hospital, Central South University, Changsha, 410011, Hunan, China. AD - Hunan Engineering Research Center of AI Medical Equipment, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China. FAU - Yu, Fenglei AU - Yu F AD - Department of Thoracic Surgery, the Second Xiangya Hospital, Central South University, Changsha, Hunan, China. yufenglei@csu.edu.cn. FAU - Wang, Lujuan AU - Wang L AD - Hunan Key Laboratory of Tumor Models and Individualized Medicine, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China. wanglujuan001@csu.edu.cn. AD - Department of Orthopaedics, The Second Xiangya Hospital, Central South University, Changsha, 410011, Hunan, China. wanglujuan001@csu.edu.cn. AD - Hunan Engineering Research Center of AI Medical Equipment, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China. wanglujuan001@csu.edu.cn. LA - eng GR - 32400991/National Natural Science Foundation of China/ PT - Journal Article PT - Review DEP - 20260801 PL - England TA - Mol Cancer JT - Molecular cancer JID - 101147698 RN - 0 (3' Untranslated Regions) RN - 0 (RNA, Messenger) SB - IM MH - Humans MH - *Polyadenylation MH - *Neoplasms/genetics/metabolism/therapy/pathology MH - *Gene Expression Regulation, Neoplastic MH - Animals MH - 3' Untranslated Regions MH - RNA, Messenger/genetics MH - Alternative Splicing COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:56 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/17 00:00 [received] PHST- 2026/07/26 00:00 [accepted] PHST- 2026/10/30 00:56 [entrez] AID - 10.1186/s12943-026-02760-0 [pii] AID - 10.1186/s12943-026-02760-0 [doi] PST - epublish SO - Mol Cancer. 2026 Aug 1;25(1):260. doi: 10.1186/s12943-026-02760-0. PMID- 42958234 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 1472-6955 (Print) IS - 1472-6955 (Linking) VI - 25 IP - 1 DP - 2026 Aug 3 TI - A Delphi study to identify competencies of registered nurses in Kenyan intensive care units. LID - 1035 [pii] LID - 10.1186/s12912-026-05112-x [doi] AB - BACKGROUND: Nurses form the largest professional group in the Intensive Care Unit (ICU) workforce. Their competency in care delivery contributes to improved patient outcomes, reduced morbidity and mortality, and overall reduced cost of care. Many countries have established frameworks to support a set of core competencies that all qualified ICU nurses are expected to possess. Kenya has yet to embark on a unifying competency framework, leading to ICU nurses' poor transfer of knowledge, skills, and attitudes because of differences in the scope and depth of competencies learned. Unification of competencies may best be achieved through stakeholders' agreement on the required set of competencies. AIM: The aim of this study was to establish consensus on the initial set of competencies required by registered nurses working in Kenyan ICUs. METHODS: The study adopted a mixed-method design with a multistage approach. This involved two stages: an integrative review, and two rounds of e-Delphi. The integrative review of competencies and competency frameworks for ICU nurses informed the development of an initial set of 91 competencies. These competencies were subsequently subjected to expert evaluation by a heterogeneous panel of experts in a two-round e-Delphi process. However, All the competencies in the two rounds achieved consensus by attaining a priori mean of >/= 4. This manuscript reports findings from the integrative review and the first round of the e-Delphi process. Findings from subsequent Delphi rounds are reported separately. Notably, despite ICU competencies being universal, some competencies are culturally specific and may require contextual tweaking for their acceptability. These include competencies touching on palliative and end-of-life care, involvement of patients and their families in pain management, and competencies that require autonomous practice of nurses, like performance of invasive procedures. CONCLUSIONS/IMPLICATION: This study identified and synthesised competencies for registered nurses in Kenyan ICUs through an integrative review and further evaluated their relevance through the first round of a Delphi process. The findings provide an initial, evidence-informed set of competencies that can inform curriculum development, clinical practice, and policy discussions. Further Delphi round will build on these findings to establish final consensus on the competency framework. CLINICAL TRIAL NUMBER: Not applicable. CI - (c) 2026. The Author(s). FAU - Waweru, Beth AU - Waweru B AD - School of Nursing & Midwifery, Aga Khan University, Nairobi, Kenya. bethngoima@gmail.com. FAU - Stephens, Melanie AU - Stephens M AD - School of Health and Society, Salford University, Greater Manchester, UK. FAU - Kamerade, Daiga AU - Kamerade D AD - School of Health and Society, Salford University, Greater Manchester, UK. FAU - Ndirangu, Eunice AU - Ndirangu E AD - School of Nursing & Midwifery, Aga Khan University, Nairobi, Kenya. FAU - Gatiti, Peter AU - Gatiti P AD - Faculty of Health Sciences, Aga Khan University, Nairobi, Kenya. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - BMC Nurs JT - BMC nursing JID - 101088683 OTO - NOTNLM OT - Competencies OT - Competency framework OT - Intensive care unit OT - Kenya OT - Registered nurses COIS- Declarations. Ethics approval and consent to participate: Ethical approvals for this study were obtained from all relevant bodies to include the University of Salford ethics Board, Aga Khan University and National Commission for Science, Technology and Innovation(NACOSTI). All procedures were carried out in compliance with the ethical standards set forth in the Declaration of Helsinki and relevant institutional guidelines. All participants provided written informed consent after receiving comprehensive details about the study's objectives, methodologies, and confidentiality measures. Participation was fully voluntary, with individuals having the freedom to withdraw from study at any point without facing any consequences. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:55 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/06/25 00:00 [received] PHST- 2026/07/21 00:00 [accepted] PHST- 2026/10/30 00:55 [entrez] AID - 10.1186/s12912-026-05112-x [pii] AID - 10.1186/s12912-026-05112-x [doi] PST - epublish SO - BMC Nurs. 2026 Aug 3;25(1):1035. doi: 10.1186/s12912-026-05112-x. PMID- 42958231 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1472-6920 (Electronic) IS - 1472-6920 (Linking) VI - 26 IP - 1 DP - 2026 Aug 1 TI - Reflective practice as a pedagogical tool for developing cultural awareness: a qualitative study in optometric education. LID - 1742 [pii] LID - 10.1186/s12909-026-10084-2 [doi] AB - BACKGROUND: Culturally responsive dispensing is increasingly recognised as a core component of contemporary optometric practice. Yet many optometry students continue to approach spectacle selection as a purely technical task, with limited engagement with the social and cultural needs of diverse patient groups. Reflective practice offers a pedagogical approach that may deepen students' awareness of these complexities. This study examined how optometry students used reflective writing to make sense of an assignment requiring them to design a spectacle frame for a specific population group. The aim was to explore how students' reflections revealed the development of cultural awareness, patient-centered thinking, and an understanding of socio-cultural influences on frame design. Specifically, the study addressed the following research question: How do optometry students, through reflective writing, make sense of cultural, social, and contextual influences on spectacle frame design, and how are patients and professional roles discursively positioned within these reflections? METHODS: Students submitted reflective essays after completing a frame-design assignment in a third-year dispensing course. Participation in the study was voluntary and occurred only after assignment grades had been released. Essays from consenting students were anonymized by an independent assistant before being analysed. A reflexive thematic analysis was conducted, informed by sociocultural learning theory and positioning theory. The analysis focused on how students made meaning, positioned themselves and patients, and engaged with cultural and contextual considerations in dispensing practice. Reflexive journaling supported the analytic process. RESULTS: Five themes were developed. Students often described an expanded understanding of dispensing as a relational and context-dependent encounter rather than a purely technical activity. Many reflected on the cultural, social, and lifestyle factors that shape spectacle frame design and patient preferences. Several students expressed greater empathy and awareness of patient individuality, particularly when designing for groups whose needs they had not previously considered. Collaborative dialogue, peer feedback, and exposure to diverse examples appeared to support deeper cultural reflection. Students also acknowledged the tensions between aesthetic ideals, functional requirements, and the cultural meanings associated with spectacle frames. DISCUSSION: Reflective practice appeared to foster cultural awareness by encouraging students to interrogate their own assumptions, recognise the socio-cultural dimensions of dispensing, and adopt more patient-centred perspectives. The reflections suggested that designing for diverse population groups can prompt critical engagement with issues of accessibility, identity, and representation. CONCLUSION: Integrating structured reflective writing into dispensing education may foster culturally responsive practice by prompting students to consider the broader contexts that shape patients' experiences and preferences. CI - (c) 2026. The Author(s). FAU - Buthelezi, Sanele AU - Buthelezi S AUID- ORCID: 0000-0003-1778-2152 AD - Department of Optometry, Faculty of Health Sciences, University of Johannesburg, Johannesburg, South Africa. saneleb@uj.ac.za. LA - eng PT - Journal Article DEP - 20260801 PL - England TA - BMC Med Educ JT - BMC medical education JID - 101088679 SB - IM MH - Humans MH - *Optometry/education MH - Cognitive Reflection MH - Qualitative Research MH - *Cultural Competency/education MH - Eyeglasses MH - Male MH - Writing MH - Female OTO - NOTNLM OT - Cultural awareness OT - Dispensing practice OT - Optometric education OT - Qualitative study OT - Reflective practice OT - Sociocultural learning OT - Spectacle frame design COIS- Declarations. Ethics approval and consent to participate: This study adhered to the Declaration of Helsinki, and ethics approval was obtained from the University of Johannesburg, Faculty of Health Sciences Research Ethics Committee (Clearance Number: REC-3908-2025). Informed consent was obtained from all respondents prior to their participation. The dataset remained confidential and was not shared or provided to any other entity. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:55 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/11/23 00:00 [received] PHST- 2026/07/30 00:00 [accepted] PHST- 2026/10/30 00:55 [entrez] AID - 10.1186/s12909-026-10084-2 [pii] AID - 10.1186/s12909-026-10084-2 [doi] PST - epublish SO - BMC Med Educ. 2026 Aug 1;26(1):1742. doi: 10.1186/s12909-026-10084-2. PMID- 42958229 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1472-6831 (Electronic) IS - 1472-6831 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Prevalence and risk factors of oral gingivitis among patients attending public and private dental clinics in Harar Town, Eastern Ethiopia: a cross-sectional study. LID - 2141 [pii] LID - 10.1186/s12903-026-09452-7 [doi] AB - BACKGROUND: Gingivitis, an early and reversible stage of periodontal disease, is a prevalent oral health problem linked to discomfort, poor quality of life, and progression to periodontitis. Despite being preventable, it remains overlooked in many low-resource settings, including Ethiopia. In Harar Town, limited data exist on its burden and associated risk factors. OBJECTIVE: To assess the prevalence of gingivitis and identify sociodemographic and behavioral risk factors among patients attending dental clinics in Harar Town, Eastern Ethiopia. METHODS: A facility-based cross-sectional study was conducted from November 21 to December 5, 2023, among 416 patients aged >/= 5 years with provision of informed consent. Participants were selected using stratified and systematic random sampling from two public and six private dental clinics. Data were collected through structured interviews and clinical examinations using the WHO's Modified Community Periodontal Index (CPI), with gingivitis defined as CPI >/= 1. Binary logistic regression identified independent predictors, with adjusted odds ratios (AORs) and 95% confidence intervals (CIs). Statistical significance was set at p < 0.05. RESULTS: Of 416 participants, 402 completed the study (96.6% response rate). The mean age was 31.86 +/- 16.32 years (range: 5-78). Gingivitis prevalence was 48.5% (95% CI: 0.44-0.54). Significant predictors included male sex (AOR = 2.21; 95% CI: 1.19-4.12), rural residence (AOR = 2.67; 95% CI: 1.36-5.26), monthly income < 3500 ETB (AOR = 2.65; 95% CI: 1.36-5.16), and illiteracy (AOR = 3.73; 95% CI: 1.37-10.14). Behavioral factors included infrequent tooth brushing (AOR = 4.51; 95% CI: 2.29-8.89), self-perceived halitosis (AOR = 2.03; 95% CI: 1.12-6.66), lack of oral health information (AOR = 2.34; 95% CI: 1.09-5.02), khat chewing (AOR = 2.65; 95% CI: 1.37-5.11), smoking (AOR = 3.63; 95% CI: 1.58-8.36), alcohol use (AOR = 4.20; 95% CI: 1.60-11.02), and frequent refined carbohydrate intake (AOR = 2.56; 95% CI: 1.19-5.49). CONCLUSION: Gingivitis is common in Harar Town and strongly associated with modifiable factors. Targeted oral health education and behavioral interventions are recommended. CI - (c) 2026. The Author(s). FAU - Admasu, Birhanu AU - Admasu B AD - Hiwot Fana Specialized University Hospital, Harar, Ethiopia. badmasu243@gmail.com. FAU - Belete, Nahom Girma AU - Belete NG AD - Hiwot Fana Specialized University Hospital, Harar, Ethiopia. FAU - Esayas, Nahom Abate AU - Esayas NA AD - GOAL Ethiopia, Addis Ababa, Ethiopia. FAU - Demena, Melake AU - Demena M AD - School of Public Health, College of Health and Medical Sciences, Haramaya University, Harar, Ethiopia. FAU - Negash, Belay AU - Negash B AD - School of Public Health, College of Health and Medical Sciences, Haramaya University, Harar, Ethiopia. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - BMC Oral Health JT - BMC oral health JID - 101088684 SB - IM MH - Humans MH - *Gingivitis/epidemiology MH - Cross-Sectional Studies MH - Ethiopia/epidemiology MH - Male MH - Risk Factors MH - Female MH - Prevalence MH - Adult MH - *Dental Clinics/statistics & numerical data MH - Adolescent MH - Middle Aged MH - Young Adult MH - Aged MH - Child OTO - NOTNLM OT - Ethiopia OT - Gingivitis OT - Harar Town OT - Oral hygiene OT - Risk factors COIS- Declarations. Ethics approval and consent to participate: The study protocol was reviewed and approved by the Institutional Health Ethics Review Committee (IHERC) of Haramaya University (Reference number: #116/2024) on May 20, 2024. All procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional research committee and with the 1964 Declaration of Helsinki and its later amendments. All participants were provided with information about the study's purpose and procedures, and written informed consent was obtained from all participants before their inclusion in the study. Consent for publication: Informed consent was obtained from all individuals who participated in the study. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:55 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/06/16 00:00 [received] PHST- 2026/07/24 00:00 [accepted] PHST- 2026/10/30 00:55 [entrez] AID - 10.1186/s12903-026-09452-7 [pii] AID - 10.1186/s12903-026-09452-7 [doi] PST - epublish SO - BMC Oral Health. 2026 Aug 3;26(1):2141. doi: 10.1186/s12903-026-09452-7. PMID- 42958227 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2490 (Electronic) IS - 1471-2490 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Integration of bulk RNA-seq data with single-cell RNA-seq data to identify urinary biomarkers derived from prostate tumor tissues. LID - 269 [pii] LID - 10.1186/s12894-026-02295-y [doi] AB - BACKGROUND: Prostate cancer (PCa) tissue biopsy has potential complications, and various methods guide biopsy decision. However, these methods have limitations. Urine-derived RNA shows promise for predicting and distinguishing PCa or clinically significant PCa (csPCa). The study aims to screen for prostate tumor cell-derived mRNAs in urine samples. METHODS: Strand-specific capture RNA sequencing (RNA-seq) was performed on five paired tumor and adjacent normal prostate tissues from PCa patients, as well as ten urine samples derived from PCa patients and age-matched cancer-free healthy men. By integrating these transcriptomic data with single-cell RNA sequencing (scRNA-seq) datasets of three normal prostate tissues and 13 PCa tissues, we systematically screened and identified potential urinary biomarkers for PCa diagnosis. To further explore the diagnostic efficacy of the screened urinary biomarkers for csPCa, quantitative real-time polymerase chain reaction (qPCR) was conducted on 63 urine samples, including 33 samples from PCa patients and 30 samples from age-matched cancer-free healthy men. Additionally, in vitro gene knockdown experiments were implemented to preliminarily explore the biological functions of the identified urinary biomarkers. RESULTS: Integrated analysis of strand-specific capture bulk RNA-seq and single-cell RNA-seq datasets identified urine-derived MARCKSL1 as a highly specific candidate biomarker for PCa diagnosis. In vitro functional assays demonstrated that genetic knockdown of MARCKSL1 robustly suppresses the migratory and invasive capacities of PCa cells. Furthermore, qPCR profiling verified that urine-derived MARCKSL1 exhibits favorable diagnostic performance, allowing accurate stratification of patients with PCa and csPCa. CONCLUSIONS: Urine-derived MARCKSL1 has the potential to facilitate pre-biopsy diagnosis and risk stratification of PCa and csPCa. Our findings may advance the development of non-invasive tools for pre-biopsy PCa risk evaluation and csPCa discrimination. CI - (c) 2026. The Author(s). FAU - Shen, Yanting AU - Shen Y AUID- ORCID: 0000-0002-9676-1562 AD - School of Gongli Hospital Medical Technology, University of Shanghai for Science and Technology, Shanghai, 200093, PR China. AD - School of clinical medicine, Shanghai University of Medicine & Health Sciences, Shanghai, 200237, PR China. FAU - Guo, Yuhan AU - Guo Y AD - School of Gongli Hospital Medical Technology, University of Shanghai for Science and Technology, Shanghai, 200093, PR China. AD - Department of Urology and andrology, Pudong Gongli Hospital, Shanghai University of Medicine & Health Sciences, Shanghai, 200135, PR China. FAU - Wu, You AU - Wu Y AD - School of Medicine, Southeast University, Nanjing, 210009, PR China. FAU - Dai, Liyang AU - Dai L AD - Department of Urology, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai, 201799, PR China. FAU - Liu, Jican AU - Liu J AD - Department of Pathology, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai, 201799, PR China. FAU - Zhu, Ting AU - Zhu T AD - Department of Pathology, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai, 201799, PR China. FAU - Xu, Junyan AU - Xu J AD - School of Gongli Hospital Medical Technology, University of Shanghai for Science and Technology, Shanghai, 200093, PR China. AD - Department of Urology and andrology, Pudong Gongli Hospital, Shanghai University of Medicine & Health Sciences, Shanghai, 200135, PR China. FAU - Li, Huifeng AU - Li H AD - Department of Urology, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai, 201799, PR China. FAU - Yang, Rui AU - Yang R AD - Wuxi Maternity and Child Health Care Hospital, Affiliated Women's Hospital of Jiangnan University, Jiangsu, 214002, PR China. ruiyang826@163.com. FAU - Cai, Zhikang AU - Cai Z AD - Department of Urology and andrology, Pudong Gongli Hospital, Shanghai University of Medicine & Health Sciences, Shanghai, 200135, PR China. czkgongli@163.com. FAU - Fei, Xiawei AU - Fei X AD - Department of Urology, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan University, Shanghai, 201799, PR China. seufxw0619@126.com. LA - eng GR - 62101319/National Natural Science Foundation of China/ GR - 2024PDWSYCQN-04/The Healthcare Talents Youth Program of Shanghai Pudong New Area/ GR - BJ2023080/the Top Talent Support Program for Young and Middle-aged People of Wuxi Health Committee/ GR - YY2023-3/Qingpu District Health Commission Discipline Talent Project of Shanghai/ GR - QWJ2024-01/Qingpu District Health Commission Project of Shanghai/ PT - Journal Article DEP - 20260803 PL - England TA - BMC Urol JT - BMC urology JID - 100968571 RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - Male MH - *Prostatic Neoplasms/urine/genetics/pathology/diagnosis MH - *Biomarkers, Tumor/urine/genetics MH - *RNA-Seq MH - Middle Aged MH - *Single-Cell Analysis MH - Aged MH - Sequence Analysis, RNA OTO - NOTNLM OT - Liquid diagnosis OT - MARCKSL1 OT - Prostate cancer OT - Single-cell RNA sequencing OT - Urine COIS- Declarations. Ethics approval and consent to participate: This study was performed in accordance with the Declaration of Helsinki and was approved by the Ethical Committee of QingPu Branch of Zhongshan Hospital (Qingyi2020-19). Written informed consent was obtained from all participants included in this study. Consent for publication: Not available. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:55 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/08 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/30 00:55 [entrez] AID - 10.1186/s12894-026-02295-y [pii] AID - 10.1186/s12894-026-02295-y [doi] PST - epublish SO - BMC Urol. 2026 Aug 3;26(1):269. doi: 10.1186/s12894-026-02295-y. PMID- 42958225 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2474 (Electronic) IS - 1471-2474 (Linking) VI - 27 IP - 1 DP - 2026 Oct 13 TI - A multimodal deep learning model for classifying early- versus late-stage osteonecrosis of the femoral head on biparametric MRI. LID - 922 [pii] LID - 10.1186/s12891-026-10544-2 [doi] AB - OBJECTIVE: Precise early- versus late-stage stratification of osteonecrosis of the femoral head (ONFH) is critical for hip-preserving treatment selection, yet conventional MRI interpretation suffers from interobserver variability and limited objectivity. This study aimed to develop a multimodal deep learning model integrating biparametric MRI (STIR and T1-FSE) with clinical parameters (including age, sex, affected side (left, right, or bilateral), and etiology) to differentiate early- from late-stage ONFH and provide objective imaging evidence. METHODS: This retrospective study included 530 patients with ONFH, yielding imaging data from 851 hips. The collected data comprised STIR sequences, T1-FSE sequences, and clinical information, including age, sex, and etiology. The dataset was randomly divided at the patient level into a training set (424 patients, 675 hips) and a test set (106 patients, 176 hips) at an 8:2 ratio. Seven models were constructed: a clinical model, a STIR model, a T1-FSE model, a bpMRI model, a STIR-clinical model, a T1-FSE-clinical model, and a bpMRI-clinical model. Early- and late-fusion strategies were used for multimodal integration. The diagnostic performance of the seven models for classifying early- versus late-stage ONFH was systematically compared, with particular emphasis on their ability to identify borderline cases at the transition between clinical stages. Model interpretability was assessed using Gradient-weighted Class Activation Mapping (Grad-CAM). RESULTS: The early-fusion bpMRI-clinical model achieved the highest AUC of 0.944 (95% CI 0.905-0.973), accuracy 90.9%, sensitivity 89.0%, and specificity 92.9%, significantly outperforming single-sequence models (all P < 0.05). The confusion matrix showed that the model misclassified 10 late-stage cases as early-stage and 6 early-stage cases as late-stage. Grad-CAM visualizations indicated that the model primarily focused on clinically relevant regions, including the necrotic lesion boundary, areas of bone marrow edema, and subchondral structural abnormalities. These attention patterns were highly consistent with the core imaging features used for early- versus late-stage ONFH stratification. CONCLUSION: This study presents an interpretable multimodal deep learning framework that integrates dual-sequence MRI with clinical parameters. The framework accurately differentiates early- from late-stage ONFH according to the ARCO classification and provides objective evidence for determining the critical threshold for hip-preservation decision-making. TRIAL REGISTRATION: The trial was registered with the Chinese Clinical Trial Registry (Registration No: ChiCTR2300075354. Sep 1, 2023). CI - (c) 2026. The Author(s). FAU - Li, Siyuan AU - Li S AD - The Third Medical College, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China. AD - Department of Orthopedic, The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510378, China. FAU - Huang, Huimin AU - Huang H AD - Department of Orthopedic, The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510378, China. FAU - He, Yetong AU - He Y AD - The Third Medical College, Guangzhou University of Chinese Medicine, Guangzhou, 510405, China. AD - Department of Orthopedic, The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510378, China. FAU - Zhang, Xiaowan AU - Zhang X AD - Department of Orthopedic, The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510378, China. FAU - Li, Xiaoxia AU - Li X AD - Department of Orthopedic, The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510378, China. FAU - Wang, Lin AU - Wang L AD - Department of Orthopedic, The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510378, China. FAU - He, Wei AU - He W AD - Department of Orthopedic, The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510378, China. AD - The National Key Discipline and the Orthopedic Laboratory, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510405, China. FAU - Han, Xiaorui AU - Han X AD - Department of Radiology, Guangzhou First People's Hospital, No. 1 Panfu Road, Yuexiu District, Guangzhou, Guangdong, 510180, China. sxllhxr2024@163.com. FAU - Huang, Xudong AU - Huang X AD - Department of Orthopedic, The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510378, China. gzhtcm1982@126.com. AD - The National Key Discipline and the Orthopedic Laboratory, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510405, China. gzhtcm1982@126.com. FAU - Chen, Gengxin AU - Chen G AD - Department of Joint Orthopedic, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, No. 111Dade Road, Yuexiu District, Guangdong, 510405, Guangzhou, China. 389207217@qq.com. AD - Department of Joint Orthopedic, Guangdong Provincial Hospital of Chinese Medicine, No. 111 Dade Road, Yuexiu District, Guangzhou, Guangdong, 510405, China. 389207217@qq.com. LA - eng GR - No. 3 [2018] of the General Office, National Administration of Traditional Chinese Medicine (Medical Administration)/National Administration of Traditional Chinese Medicine (Medical Administration);/ GR - No. A2025259/the Guangdong Medical Science and Technology Research Fund/ GR - No. 2025KTSCX024/the Characteristic Innovation Project of Guangdong Provincial Universities/ GR - No. 2024A04J5056/the Guangzhou Science and Technology Program/ GR - No. B2020030/the Medical Scientific Research Foundation of Guangdong Province/ PT - Comparative Study PT - Journal Article DEP - 20261013 PL - England TA - BMC Musculoskelet Disord JT - BMC musculoskeletal disorders JID - 100968565 SB - IM MH - Humans MH - *Femur Head Necrosis/diagnostic imaging/classification MH - Female MH - *Deep Learning MH - Retrospective Studies MH - Male MH - *Magnetic Resonance Imaging/methods MH - Adult MH - Middle Aged MH - Femur Head/diagnostic imaging MH - Young Adult OTO - NOTNLM OT - Early fusion OT - Early-stage and late-stage disease OT - Hip-preserving surgery OT - Multimodal deep learning OT - Osteonecrosis of the femoral head COIS- Declarations. Ethics approval and consent to participate: This study was reviewed and approved by the Institutional Review Board (IRB) of the Third Affiliated Hospital of Guangzhou University of Chinese Medicine (Approval No: PJ-KY-20230919-001). The trial was prospectively registered with the Chinese Clinical Trial Registry (Registration No: ChiCTR2300075354. Sep 1, 2023) and documented in the National Medical Research Registration System. The study was conducted in accordance with the Declaration of Helsinki. A waiver of informed consent was granted due to the retrospective nature of the study. All patient information was anonymized and de-identified. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:55 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/06/24 00:00 [received] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/30 00:55 [entrez] AID - 10.1186/s12891-026-10544-2 [pii] AID - 10.1186/s12891-026-10544-2 [doi] PST - epublish SO - BMC Musculoskelet Disord. 2026 Oct 13;27(1):922. doi: 10.1186/s12891-026-10544-2. PMID- 42958223 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2482 (Electronic) IS - 1471-2482 (Linking) VI - 26 IP - 1 DP - 2026 Aug 1 TI - Prophylactic antibiotics for pancreaticoduodenectomy: study protocol for a multicenter randomized controlled trial comparing tazobactam/piperacillin versus ampicillin/sulbactam. LID - 726 [pii] LID - 10.1186/s12893-026-04092-8 [doi] AB - BACKGROUND: Pancreaticoduodenectomy (PD) is associated with a high incidence of surgical site infection (SSI), reported in 20-40% of cases. In patients undergoing preoperative biliary drainage (PBD), the risk is further increased due to biliary contamination. Tazobactam/piperacillin (TAZ/PIPC) has been shown to reduce SSI compared with cephalosporins; however, no prospective trial has directly compared TAZ/PIPC with ampicillin/sulbactam (ABPC/SBT), which is widely used in pancreatic surgery. This study aims to evaluate the superiority of TAZ/PIPC over ABPC/SBT in preventing SSI after PD. METHODS: This is a multicenter, open-label, randomized controlled trial. A total of 320 patients undergoing PD will be randomized (1:1) to receive either TAZ/PIPC or ABPC/SBT as perioperative prophylactic antibiotics. Randomization will be stratified by PBD, minimally invasive surgery (MIS), and pancreatic ductal adenocarcinoma (PDAC). Antibiotics will be administered within 60 min before incision, redosed intraoperatively every 2-4 h, and continued every 8 h until postoperative day 1. The primary endpoint is the incidence of SSI within 30 days postoperatively, defined according to CDC criteria. Secondary endpoints include postoperative complications (Clavien-Dindo >/=IIIa), clinically relevant postoperative pancreatic fistula (ISGPS grade B/C), delayed gastric emptying, postpancreatectomy hemorrhage, bile leakage, mortality, reoperation, readmission, C. difficile colitis, and detection of antimicrobial-resistant organisms. DISCUSSION: This trial will provide the first direct comparison between TAZ/PIPC and ABPC/SBT in PD. The results are expected to contribute to optimizing perioperative antibiotic strategies while balancing SSI prevention and antimicrobial resistance. TRIAL REGISTRATION: Japan Registry of Clinical Trials (jRCT), jRCTs051260063. CI - (c) 2026. The Author(s). FAU - Mizumoto, Takuya AU - Mizumoto T AD - Department of Surgery, Division of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-Cho, Chuo-ku, Kobe, 650-0017, Japan. FAU - Nanno, Yoshihide AU - Nanno Y AD - Department of Surgery, Division of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-Cho, Chuo-ku, Kobe, 650-0017, Japan. ynanno@med.kobe-u.ac.jp. FAU - Ishida, Jun AU - Ishida J AD - Department of Surgery, Division of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-Cho, Chuo-ku, Kobe, 650-0017, Japan. FAU - Ueda, Yuki AU - Ueda Y AD - Department of Surgery, Division of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-Cho, Chuo-ku, Kobe, 650-0017, Japan. FAU - Lee, Dongha AU - Lee D AD - Department of Surgery, Division of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-Cho, Chuo-ku, Kobe, 650-0017, Japan. FAU - Akita, Masayuki AU - Akita M AD - Department of Surgery, Division of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-Cho, Chuo-ku, Kobe, 650-0017, Japan. FAU - Asakura, Riki AU - Asakura R AD - Department of Surgery, Division of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-Cho, Chuo-ku, Kobe, 650-0017, Japan. FAU - Nakamura, Hiroyuki AU - Nakamura H AD - Department of Surgery, Division of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-Cho, Chuo-ku, Kobe, 650-0017, Japan. FAU - Okawa, Taisuke AU - Okawa T AD - Department of Surgery, Division of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-Cho, Chuo-ku, Kobe, 650-0017, Japan. FAU - Yokota, Masaharu AU - Yokota M AD - Department of Surgery, Division of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-Cho, Chuo-ku, Kobe, 650-0017, Japan. FAU - Hamano, Koki AU - Hamano K AD - Department of Surgery, Division of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-Cho, Chuo-ku, Kobe, 650-0017, Japan. FAU - Komatsu, Shohei AU - Komatsu S AD - Department of Surgery, Division of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-Cho, Chuo-ku, Kobe, 650-0017, Japan. LA - eng PT - Clinical Trial Protocol PT - Journal Article DEP - 20260801 PL - England TA - BMC Surg JT - BMC surgery JID - 100968567 RN - S4TF6I2330 (Sulbactam) RN - 0 (Anti-Bacterial Agents) RN - 157044-21-8 (Piperacillin, Tazobactam Drug Combination) RN - 7C782967RD (Ampicillin) RN - 65DT0ML581 (sultamicillin) SB - IM MH - Humans MH - *Pancreaticoduodenectomy/adverse effects MH - *Sulbactam/therapeutic use MH - *Antibiotic Prophylaxis/methods MH - *Surgical Wound Infection/prevention & control MH - *Anti-Bacterial Agents/therapeutic use MH - *Piperacillin, Tazobactam Drug Combination/therapeutic use MH - *Ampicillin/therapeutic use MH - Multicenter Studies as Topic MH - Randomized Controlled Trials as Topic MH - Pancreatic Neoplasms/surgery MH - Equivalence Trials as Topic MH - Prospective Studies MH - Female OTO - NOTNLM OT - Ampicillin/sulbactam OT - Antibiotics OT - Pancreatoduodenectomy OT - Prophylaxis OT - Surgical site infection OT - Tazobactam/piperacillin COIS- Declarations. Ethics approval and consent to participate: Oral and written informed consent will be obtained for every participant. The study protocol (currently version 1.4) was approved by the ethical committee of Kobe University on December 8th, 2025. The study will be performed in accordance with the Kobe University Conflicts of Interest Management Guidelines and adhered to the principles outlined in the Declaration of Helsinki (2013 revision). Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:55 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/06/27 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/30 00:55 [entrez] AID - 10.1186/s12893-026-04092-8 [pii] AID - 10.1186/s12893-026-04092-8 [doi] PST - epublish SO - BMC Surg. 2026 Aug 1;26(1):726. doi: 10.1186/s12893-026-04092-8. PMID- 42958222 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1472-6947 (Electronic) IS - 1472-6947 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Data-driven identification of modifiable risk factors for colorectal cancer in Palestine using machine learning methods. LID - 442 [pii] LID - 10.1186/s12911-026-03743-3 [doi] AB - BACKGROUND: Colorectal cancer (CRC) is a growing public health concern in Palestine, yet limited evidence is available on locally relevant behavioral, dietary, socioeconomic, and contextual predictors. Machine learning (ML) may support prevention-oriented risk stratification by identifying complex predictive patterns among potentially modifiable factors. This study aimed to identify important predictors of CRC among Palestinian adults and evaluate ML models for CRC classification after excluding screening-related variables. METHODS: A case-control dataset of 216 participants was analyzed, including 108 CRC cases and 108 cancer-free controls. Initially, 57 variables were reviewed, and 39 candidate predictors were retained after excluding non-modifiable variables and the outcome. Screening-related variables, including previous colonoscopy and fecal occult blood testing, were excluded from the primary modeling analysis because they may reflect diagnostic workup, screening access, or healthcare utilization. Multiple feature-selection methods were evaluated, including filter, wrapper, embedded, and hybrid approaches. Ten optimized ML classifiers were trained and internally validated using stratified 5-fold nested cross-validation, with preprocessing, feature selection, hyperparameter optimization, and model training performed within the training folds. Model performance was assessed using accuracy, AUC, sensitivity, specificity, F1-score, and Matthews correlation coefficient. SHAP analysis was used to interpret the optimized Ensemble models following hybrid feature selection. RESULTS: After excluding screening-related variables, the best model-combination performance was achieved by Forward Selection with the Ensemble classifier, with an accuracy of 90.3% +/- 4.3, a balanced accuracy of 90.28%, a sensitivity of 89.81% +/- 5.59, and a specificity of 90.74% +/- 4.18. Hybrid feature-selection approaches also showed competitive performance with Ensemble learning. Hybrid LASSO + GA with Ensemble and Hybrid mRMR + PSO with Ensemble both achieved a balanced accuracy of 89.81%. SHAP analysis consistently identified physical activity as the most influential predictor, followed by dietary, socioeconomic, and contextual variables, including fruit consumption frequency, yearly income, occupation, and place of residence. CONCLUSIONS: After excluding screening-related variables, ML models that relied on lifestyle, socioeconomic, and contextual predictors showed promising internal validation performance in classifying CRC among Palestinian adults. Hybrid feature-selection methods combined with Ensemble learning represent promising candidate pipelines for future validation. However, the findings remain exploratory and require external validation in larger, independent cohorts before clinical or public health implementation. CI - (c) 2026. The Author(s). FAU - Abuzuhri, Mohammad-Ali AU - Abuzuhri MA AUID- ORCID: 0009-0003-7304-9614 AD - RISE-Health, Department of Community Medicine, Information and Health Decision Sciences, Faculty of Medicine, University of Porto, Porto, Portugal. mohammad.zedan@aaup.edu. AD - Faculty of Nursing, Arab American University, Jenin, Palestine. mohammad.zedan@aaup.edu. FAU - Awad, Mohammed AU - Awad M AD - Department of Computer Systems Engineering, Arab American University, Jenin, Palestine. FAU - Najjar, Shahenaz AU - Najjar S AD - Department of Computer Science, KU Leuven, Leuven, Belgium. AD - Augment, IMEC Research Group, KU Leuven, Leuven, Belgium. FAU - Correia, Ricardo-Cruz AU - Correia RC AD - RISE-Health, Department of Community Medicine, Information and Health Decision Sciences, Faculty of Medicine, University of Porto, Porto, Portugal. FAU - Sharif, Nuha El AU - Sharif NE AD - Faculty of Public Health, Al Quds University, East Jerusalem, Palestine. AD - Ministry of Health, Ramallah, Palestine. FAU - Ghrouz, Issa AU - Ghrouz I AD - Ministry of Health, Ramallah, Palestine. FAU - Vieira-Marques, Pedro AU - Vieira-Marques P AD - RISE-Health, Department of Community Medicine, Information and Health Decision Sciences, Faculty of Medicine, University of Porto, Porto, Portugal. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - BMC Med Inform Decis Mak JT - BMC medical informatics and decision making JID - 101088682 SB - IM MH - Humans MH - *Colorectal Neoplasms/diagnosis/epidemiology MH - Female MH - Risk Factors MH - Middle Aged MH - *Machine Learning MH - Case-Control Studies MH - Male MH - Predictive Learning Models MH - Adult MH - *Arabs/statistics & numerical data MH - Classification Algorithms MH - Aged OTO - NOTNLM OT - Colorectal cancer OT - Explainable artificial intelligence OT - Feature selection OT - Machine learning OT - Palestine OT - Risk factors OT - Risk prediction OT - SHAP COIS- Declarations. Institutional Review Board statement (IRB): The study was conducted in accordance with the Declaration of Helsinki and approved by the Ethics Committee of the Faculty of Medicine of the University of Porto (FMUP), Portugal. Approval code (333/CEFMUP/2025) was obtained on 29th April 2025. However, human ethics and consent to participate are not applicable, since we are using anonymized secondary data with ethical approval in place. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:55 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/05 00:00 [received] PHST- 2026/07/29 00:00 [accepted] PHST- 2026/10/30 00:55 [entrez] AID - 10.1186/s12911-026-03743-3 [pii] AID - 10.1186/s12911-026-03743-3 [doi] PST - epublish SO - BMC Med Inform Decis Mak. 2026 Aug 3;26(1):442. doi: 10.1186/s12911-026-03743-3. PMID- 42958215 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2474 (Electronic) IS - 1471-2474 (Linking) VI - 27 IP - 1 DP - 2026 Aug 1 TI - Anterolateral leg wide-pedicle dual-vascular flap for reconstruction of traumatic midfoot and forefoot soft-tissue defects: a retrospective case series. LID - 924 [pii] LID - 10.1186/s12891-026-10263-8 [doi] AB - BACKGROUND: Traumatic midfoot and forefoot soft-tissue defects are difficult to reconstruct because local tissue is thin, deep structures are frequently exposed, and distal pedicled flaps may develop ischaemia or venous congestion. We evaluated the clinical outcomes of an anterolateral leg wide-pedicle dual-vascular flap based on the terminal perforator of the peroneal artery and the vascular plexus accompanying the superficial peroneal nerve. METHODS: We retrospectively reviewed 16 consecutive patients treated between September 2015 and June 2021. Data collected from operative and follow-up records included demographic and injury characteristics, exposed structures, associated osseous injury, defect and flap size, injury-to-coverage interval, operative time, flap-related events, infection, wound or graft dehiscence, donor-site healing, scar-related morbidity when documented, ankle range of motion, and American Orthopaedic Foot and Ankle Society (AOFAS) midfoot score. Flap vascularity during follow-up was reassessed clinically rather than by routine vascular imaging. Results were summarized descriptively. RESULTS: The cohort comprised 12 men and 4 women, with a mean age of 37.9 years (range 22-53 years). Injury mechanisms were traffic accidents in 6 patients and crush injuries in 10. Three patients had isolated soft-tissue defects, whereas 13 had associated fractures, dislocations, or bone defects. Exposed tendon was present in 8 patients and exposed bone in 9. Defect size ranged from 4 x 5 cm to 7 x 12 cm and flap size from 5 x 7 cm to 8 x 14 cm. Mean operative time was 100.1 min (range 75-130 min). All 16 flaps survived. One patient developed early vascular compromise on postoperative day 2 that resolved after pedicle suture release and superficial decompression; no partial flap necrosis, total flap loss, infection, wound dehiscence, donor-site graft loss requiring regrafting, or clinically relevant scar contracture was documented. Mean follow-up was 14.9 months (range 6-24 months). Four patients had mild residual pedicle oedema. All donor sites healed after split-thickness skin grafting. Final ankle dorsiflexion ranged from 15 to 20 degrees and plantar flexion from 30 to 40 degrees. Mean AOFAS midfoot score was 83.1 (range 75-90). Standardized sensory testing, pain scores, footwear tolerance, and return-to-activity measures were not consistently available in the retrospective records. CONCLUSIONS: In this small retrospective series, the anterolateral leg wide-pedicle dual-vascular flap provided reliable coverage for selected traumatic midfoot and forefoot defects without microvascular anastomosis. These findings should be interpreted as descriptive evidence rather than proof of superiority over established regional or free flaps. The proposed vascular mechanism and the sensory consequences of superficial peroneal nerve division require further dedicated evaluation. TRIAL REGISTRATION: Not applicable. CI - (c) 2026. The Author(s). FAU - Shi, Xianqing AU - Shi X AD - Department of Orthopaedic Surgery, The 940th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army, Lanzhou, Gansu, China. AD - Graduate School, Gansu University of Traditional Chinese Medicine, Lanzhou, Gansu, China. FAU - Xue, Yun AU - Xue Y AD - Department of Orthopaedic Surgery, The 940th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army, Lanzhou, Gansu, China. FAU - Liu, Lihong AU - Liu L AD - Department of Orthopaedic Surgery, The 940th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army, Lanzhou, Gansu, China. FAU - Deng, Xiaowen AU - Deng X AD - Department of Orthopaedic Surgery, The 940th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army, Lanzhou, Gansu, China. FAU - Li, Wenshuai AU - Li W AD - Department of Orthopaedic Surgery, The 940th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army, Lanzhou, Gansu, China. AD - Graduate School, Gansu University of Traditional Chinese Medicine, Lanzhou, Gansu, China. FAU - Wang, Wei AU - Wang W AD - Department of Orthopaedic Surgery, The 940th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army, Lanzhou, Gansu, China. FAU - Shi, Jie AU - Shi J AD - Department of Orthopaedic Surgery, The 940th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army, Lanzhou, Gansu, China. FAU - Shi, Peisheng AU - Shi P AD - Department of Orthopaedic Surgery, The 940th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army, Lanzhou, Gansu, China. FAU - Zhang, Yaqiang AU - Zhang Y AD - Department of Orthopaedic Surgery, The 940th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army, Lanzhou, Gansu, China. FAU - Mou, Guisheng AU - Mou G AD - Department of Orthopaedic Surgery, The 940th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army, Lanzhou, Gansu, China. AD - Graduate School, Gansu University of Traditional Chinese Medicine, Lanzhou, Gansu, China. FAU - Li, Wenbo AU - Li W AD - Department of Orthopaedic Surgery, The 940th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army, Lanzhou, Gansu, China. dliwenb@163.com. FAU - Gao, Qiuming AU - Gao Q AD - Department of Orthopaedic Surgery, The 940th Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army, Lanzhou, Gansu, China. gaoqm001@sohu.com. LA - eng GR - 23YFFA0055/Science and Technology Program of Gansu Province/ GR - 21JR7RA016/Science and Technology Program of Gansu Province/ GR - (2024-9-160)/the Lanzhou Science and Technology Plan Project/ GR - 25JRRA1182/Gansu Provincial Joint Research Fund Major Project/ PT - Journal Article DEP - 20260801 PL - England TA - BMC Musculoskelet Disord JT - BMC musculoskeletal disorders JID - 100968565 SB - IM MH - Humans MH - Female MH - Male MH - Adult MH - Retrospective Studies MH - *Soft Tissue Injuries/surgery MH - Middle Aged MH - *Plastic Surgery Procedures/methods/adverse effects MH - Young Adult MH - *Surgical Flaps/blood supply MH - *Foot Injuries/surgery MH - Treatment Outcome MH - *Forefoot, Human/surgery/injuries MH - Follow-Up Studies OTO - NOTNLM OT - Anterolateral leg flap OT - Forefoot OT - Midfoot OT - Peroneal artery perforator OT - Reconstruction OT - Soft-tissue defect OT - Superficial peroneal nerve COIS- Declarations. Ethics approval and consent to participate: The study was approved by the institutional research ethics committee of the 940th Hospital of Logistics Support Force of PLA (No. 2023KYLL225). All procedures were performed in accordance with the Declaration of Helsinki. Written informed consent to participate was obtained according to the original clinical documentation. Consent for publication: Written informed consent for publication of the de-identified clinical images presented in Fig. 1 was obtained from the patient whose images are shown and is retained by the authors in accordance with journal policy. Only de-identified clinical images for which publication consent was available are included in the manuscript. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:54 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2024/10/28 00:00 [received] PHST- 2026/07/20 00:00 [accepted] PHST- 2026/10/30 00:54 [entrez] AID - 10.1186/s12891-026-10263-8 [pii] AID - 10.1186/s12891-026-10263-8 [doi] PST - epublish SO - BMC Musculoskelet Disord. 2026 Aug 1;27(1):924. doi: 10.1186/s12891-026-10263-8. PMID- 42958207 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2458 (Electronic) IS - 1471-2458 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Development, implementation, and evaluation of a belief-targeted non-face-to-face leisure-time physical activity promotion program for South Korean University students. LID - 3130 [pii] LID - 10.1186/s12889-026-28797-w [doi] AB - BACKGROUND: Despite a sharp decline in physical activity levels among university students since the COVID-19 pandemic, physical activity programs for this population remain limited. Our previous research examined key beliefs influencing physical activity behavior among university students at a Korean university during the COVID-19 pandemic. Accordingly, this study aimed to develop, implement, and evaluate a non-face-to-face leisure-time physical activity (NFTF-LTPA) promotion program for university students, focusing on three beliefs-health, friends, and laziness-identified as key determinants of physical activity behavior in our previous study. METHODS: Undergraduate students were newly recruited from the same university and departments (i.e., the College of Social Sciences and the School of Business) as our prior study. Participants were stratified and randomly assigned to intervention or control groups and a belief-targeted intervention was implemented for three months. Process evaluations were conducted at mid-, and post-intervention, and outcomes were assessed at pre-, mid-, and post-intervention. A one-month follow-up survey was also conducted after the intervention ended. A 2 x 4 repeated measures generalized linear model was conducted and subgroup analyses examined participants with high intervention adherence (PHIA) compared with controls. RESULTS: Among the total analytic sample, significant group x time interaction effects at the alpha = 0.10 level were observed for outcome evaluation of behavioral beliefs (F = 2.70; p = 0.072; [Formula: see text]= 0.05) and motivation to comply with normative beliefs (F = 2.54; p = 0.085; [Formula: see text]= 0.05). However, no significant group x time interaction effects were observed for control beliefs, intention, or LTPA participation. In an exploratory subgroup analysis, a significant group x time interaction effect was observed for the control belief of laziness (F = 3.85; p = 0.031; [Formula: see text] = 0.18). Post hoc analyses indicated lower perceived laziness at the program midpoint in the PHIA group compared with the control group. CONCLUSIONS: Although the intervention produced changes in selected belief components among the total analytic sample, these changes did not translate into significant improvements in intention or behavior. Exploratory subgroup analyses suggested potential changes in control beliefs among participants with higher intervention adherence. These findings provide preliminary insights into the development, implementation, and evaluation of belief-targeted NFTF-LTPA promotion programs for university students. TRIAL REGISTRATION: The trial was retrospectively registered at ClinicalTrials.gov (Identifier: NCT07423403; 19 February 2026). CI - (c) 2026. The Author(s). FAU - Kwon, Junhye AU - Kwon J AD - Department of Physical Education, College of Education, 71-1, Seoul National University, Seoul, 08826, South Korea. FAU - Kang, Joon-Ho AU - Kang JH AD - Department of Physical Education, College of Education, 71-1, Seoul National University, Seoul, 08826, South Korea. FAU - Lee, Chung Gun AU - Lee CG AD - Department of Physical Education, College of Education, 71-1, Seoul National University, Seoul, 08826, South Korea. cgl81@snu.ac.kr. AD - Institute of Sport Science, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, South Korea. cgl81@snu.ac.kr. LA - eng SI - ClinicalTrials.gov/NCT07423403 PT - Journal Article PT - Randomized Controlled Trial DEP - 20260803 PL - England TA - BMC Public Health JT - BMC public health JID - 100968562 SB - IM MH - Humans MH - Universities MH - *Students/psychology/statistics & numerical data MH - Female MH - *Health Promotion/methods/organization & administration MH - Male MH - Republic of Korea MH - *Exercise/psychology MH - *Leisure Activities/psychology MH - Young Adult MH - Program Evaluation MH - Program Development MH - Adult MH - COVID-19/epidemiology OTO - NOTNLM OT - Belief-targeted intervention OT - Leisure time physical activity OT - Physical activity promotion program OT - Program evaluation OT - University students COIS- Declarations. Ethics approval and consent to participate: This study was approved by the Seoul National University Institutional Review Board (IRB No. 2301/002-012) and was conducted in accordance with the Declaration of Helsinki. Written consent was obtained from all participants and all methods were conducted according to relevant guidelines. Participant information was kept confidential and used only for research purposes. All participants were informed about the study procedures if they agreed to participate in the study. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:54 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/01/19 00:00 [received] PHST- 2026/07/24 00:00 [accepted] PHST- 2026/10/30 00:54 [entrez] AID - 10.1186/s12889-026-28797-w [pii] AID - 10.1186/s12889-026-28797-w [doi] PST - epublish SO - BMC Public Health. 2026 Aug 3;26(1):3130. doi: 10.1186/s12889-026-28797-w. PMID- 42958206 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2662-7671 (Electronic) IS - 2662-7671 (Linking) VI - 26 IP - 1 DP - 2026 Aug 1 TI - Developing evidence-based criteria for selecting herbal medicines for the national list of essential medicines. LID - 326 [pii] LID - 10.1186/s12906-026-05494-0 [doi] AB - BACKGROUND: The National List of Essential Medicines in Thailand selects herbal medicines through a consensus-based process, in which experts independently assess the evidence. However, there is a lack of standardised criteria for evaluating the evidence of efficacy and safety. This study aimed to develop two sets of modified criteria-one for traditional medicines and another for herbal medicines derived from traditional use-focusing on evidence-based efficacy and safety. METHODS: A mixed-methods study was conducted. The criteria were modified for decision-making regarding herbal medicines based on evidence and the 2009 National Health and Medical Research Council guidelines. They were also aligned with the World Health Organisation guidelines for assessing herbal medicines, the European Medicines Agency regulations on traditional herbal medicines, the Australian guidelines for levels and kinds of evidence to support claims for therapeutics goods, and the standards of the National Center for Complementary and Alternative Medicine. Content and face validity were assessed, and interrater reliability was tested. RESULTS: The efficacy criteria consist of six aspects: levels of evidence, study outcomes, consistency of the evidence, clinical significance, availability of herbal products in Thailand, and rational application of herbal medicine. The safety criteria include seven components: adverse effects of herbal medicines from post-marketing surveillance; safety information from herbal monographs or the National Thai Traditional Medicine Formulary; meta-analyses or systematic reviews; clinical trials or pragmatic trials; observational studies; case reports; and preclinical studies. Each component of the efficacy and safety criteria consists of three decision-making levels: accepted, requires more information, and rejected/expert judgment. The mean item-objective congruence for the criteria for traditional medicines and herbal products was 0.93 and 0.74, respectively. The kappa coefficients of interrater agreement were 0.74 and 0.71. CONCLUSIONS: The criteria clearly identified traditional and herbal medicines into three categories: 1) fully accepted for herbal medicines with robust evidence of efficacy and safety; 2) herbal medicines requiring additional information to prove their efficacy or safety; and 3) rejected herbal medicines lacking evidence of efficacy or safety. CI - (c) 2026. The Author(s). FAU - Tangkiatkumjai, Mayuree AU - Tangkiatkumjai M AUID- ORCID: 0000-0002-9442-970X AD - Department of Clinical Pharmacy, Faculty of Pharmacy, Srinakharinwirot University, Nakhon Nayok, 26120, Thailand. mayureet@g.swu.ac.th. FAU - Kamkaen, Narisa AU - Kamkaen N AD - The College of Herbal Pharmacy of Thailand (Under the Royal College of Pharmacy of Thailand), Chulabhorn Royal Academy, Bangkok, Thailand. FAU - Pakdeesattayapong, Duangkamol AU - Pakdeesattayapong D AD - Herbal Products Division, Thai Food and Drug Administration, Nonthaburi, Thailand. FAU - Namthongbai, Nidchakarn AU - Namthongbai N AD - Herbal Products Division, Thai Food and Drug Administration, Nonthaburi, Thailand. FAU - Theantawee, Wannisa AU - Theantawee W AD - The National Drug Policy Division, Thai Food and Drug Administration, Nonthaburi, Thailand. FAU - Sakthong, Phantipa AU - Sakthong P AD - Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Pathumwan, 10330, Thailand. phantipa.s@pharm.chula.ac.th. LA - eng PT - Journal Article DEP - 20260801 PL - England TA - BMC Complement Med Ther JT - BMC complementary medicine and therapies JID - 101761232 RN - 0 (Drugs, Essential) SB - IM MH - Thailand MH - Humans MH - *Drugs, Essential/standards MH - *Evidence-Based Medicine MH - *Phytotherapy/standards MH - *Herbal Medicine/standards MH - *Medicine, Thai Traditional OTO - NOTNLM OT - Herbal Medicine OT - National List of Essential Medicines OT - Thailand COIS- Declarations. Ethics approval and consent to participate: Ethical approval (number SWUEC/E-017/2566) was obtained from the institutional review board of Srinakharinwirot University in Thailand. The study was conducted in accordance with the Declaration of Helsinki. All participants provided informed consent. Consent for publication: Not applicable. Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:54 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2024/11/26 00:00 [received] PHST- 2026/07/24 00:00 [accepted] PHST- 2026/10/30 00:54 [entrez] AID - 10.1186/s12906-026-05494-0 [pii] AID - 10.1186/s12906-026-05494-0 [doi] PST - epublish SO - BMC Complement Med Ther. 2026 Aug 1;26(1):326. doi: 10.1186/s12906-026-05494-0. PMID- 42958203 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2458 (Electronic) IS - 1471-2458 (Linking) VI - 26 IP - 1 DP - 2026 Aug 1 TI - Prevalence and reporting barriers of child sexual abuse among in-school children and adolescents in Ibadan Metropolis, Oyo State, Nigeria: a cross-sectional mixed-methods study. LID - 3106 [pii] LID - 10.1186/s12889-026-28871-3 [doi] AB - BACKGROUND: Child sexual abuse (CSA) is a major public health and human rights concern, with underreporting limiting accurate estimation and effective prevention and response efforts, particularly in low- and middle-income countries. Evidence on the prevalence of contact, non-contact, and technology-facilitated CSA and the barriers to reporting among school-aged populations in Nigeria remains limited. This study assessed the prevalence and patterns of CSA and examined intrapersonal barriers to reporting among in-school children and adolescents in Ibadan, Nigeria. METHODS: A cross-sectional mixed-methods study was conducted among 460 randomly selected in-school children and adolescents aged 8-17 years attending eight public secondary schools in Ibadan, south-west Nigeria. Data were collected using a pretested interviewer-administered questionnaire adapted from previously validated instruments. Quantitative data were analysed descriptively using SPSS version 23. Responses to open-ended questions were analysed thematically to complement quantitative findings and explore adolescents' knowledge, attitudes, and perceived intrapersonal barriers to reporting CSA. RESULTS: Overall, 25.2% of respondents reported experiencing at least one form of CSA. Non-contact and technology-facilitated forms of abuse were the most frequently reported, particularly exposure to pornographic materials (71.6%) and sexting (37.9%). More than 40% of respondents who experienced CSA had not disclosed their experiences. Although most respondents demonstrated good knowledge of reporting mechanisms and positive attitudes towards reporting, qualitative findings revealed persistent intrapersonal barriers to disclosure, including fear of punishment or retaliation, shame, perceived stigma, threats by perpetrators, close relationships with perpetrators, secrecy, and limited trust in adults or authorities. Many respondents also demonstrated a narrow conceptualisation of CSA, often restricting it to penetrative abuse. CONCLUSION: CSA Child sexual abuse remains a substantial public health concern among in-school children and adolescents in Ibadan, with non-contact and technology-facilitated forms constituting a considerable proportion of reported experiences. Strengthening comprehensive sexuality education, digital safety education, adolescent-friendly reporting systems, and supportive responses from families, schools, and communities may improve recognition, disclosure, and child protection outcomes. CI - (c) 2026. The Author(s). FAU - Osiberu-Asaolu, Adenike AU - Osiberu-Asaolu A AD - Department of Public Health, College of Basic Medical Sciences, Chrisland University, Abeokuta, Nigeria. adenikeosiberu@gmail.com. AD - Department of Health Promotion and Education, Faculty of Public Health, College of Medicine, University of Ibadan, Ibadan, Nigeria. adenikeosiberu@gmail.com. FAU - Oladepo, Oladimeji AU - Oladepo O AD - Department of Health Promotion and Education, Faculty of Public Health, College of Medicine, University of Ibadan, Ibadan, Nigeria. LA - eng PT - Journal Article DEP - 20260801 PL - England TA - BMC Public Health JT - BMC public health JID - 100968562 SB - IM MH - Humans MH - Nigeria/epidemiology MH - Adolescent MH - Child MH - Cross-Sectional Studies MH - Female MH - *Child Abuse, Sexual/statistics & numerical data/psychology MH - Male MH - Prevalence MH - Surveys and Questionnaires MH - Health Knowledge, Attitudes, Practice MH - Schools OTO - NOTNLM OT - Adolescents OT - Children OT - Knowledge OT - Nigeria OT - Perception OT - Prevalence OT - Reporting barriers OT - Sexual abuse COIS- Declarations. Ethics approval and consent to participate: Ethical approval was obtained from the Oyo State Research Ethics Review Committee (Ref: AD 13/479/1251), and the research was conducted in accordance with the ethical principles outlined in the Declaration of Helsinki (1964) and its subsequent amendments. Administrative permission was also secured from the Oyo State education authorities and participating school principals prior to data collection. Written informed consent was obtained from parents or guardians, and assent was obtained from participating adolescents. Participants were clearly informed about the purpose of the study, the voluntary nature of participation, and their right to decline participation or withdraw at any point without any adverse consequences. Given the sensitive nature of child sexual abuse, confidentiality and privacy were prioritised throughout the research process. Data collection was conducted in a respectful and age-appropriate manner, and no identifying information was included in the analysis or reporting of findings. Interviewers were trained to conduct the study with sensitivity and to recognise signs of emotional discomfort. Where distress was observed or abuse was disclosed, appropriate referral pathways, including school counselling services and child protection support mechanisms, were activated in line with ethical guidance. All data were handled securely and used solely for research purposes. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:54 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/11 00:00 [received] PHST- 2026/07/30 00:00 [accepted] PHST- 2026/10/30 00:54 [entrez] AID - 10.1186/s12889-026-28871-3 [pii] AID - 10.1186/s12889-026-28871-3 [doi] PST - epublish SO - BMC Public Health. 2026 Aug 1;26(1):3106. doi: 10.1186/s12889-026-28871-3. PMID- 42958202 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2474 (Electronic) IS - 1471-2474 (Linking) VI - 27 IP - 1 DP - 2026 Aug 3 TI - The relationship between in-socket mechanics and patient-reported comfort and function, a randomized crossover trial. LID - 932 [pii] LID - 10.1186/s12891-026-10313-1 [doi] AB - BACKGROUND: Lower limb loss presents significant challenges, resulting in chronic health issues and impacting the quality of life. Prosthetic socket design is crucial for optimizing comfort and function in individuals with limb loss. METHODS: This study aimed to determine the relationship between in-socket mechanics and patient-reported comfort and function, hypothesizing that residual femur motion, skin strain, and pressure distribution correlate with these outcomes. Ten individuals with transfemoral limb loss participated, with modifications made to personalized prosthetic check sockets to assess these variables, and data from eight subjects were included in the analysis. Data collection included CT scans, dynamic biplane radiography, in-socket pressure measurements, plantar pressure assessments, ground reaction force analyses, conventional motion capture assessments, and patient-reported outcomes. Generalized estimated equations were used to examine the relationships between peak skin strain during gait and patient reported outcomes and correlation matrices were constructed to explore relationships between static imaging, skin strain, residual bone motion, and gait kinematics. RESULTS: Our findings indicate that the combination of skin strain metrics significantly predicts variability in comfort, while the overall model was not significant with respect to function. Significant correlations were also observed between static imaging and dynamic residual bone motion. CONCLUSIONS: These results suggest that minimizing and monitoring skin strain, particularly in the anterior central socket region, can enhance prosthetic fit and patient satisfaction. Additionally, static imaging under different loads effectively predicts dynamic displacements, offering a less invasive method for clinical assessments. These insights highlight the need for integrating objective in-socket measurements into clinical practice to improve prosthetic socket fitting, ultimately enhancing the quality of life for individuals with lower limb loss. TRIAL REGISTRATION: ClinicalTrials.gov NCT05041998. Date of registration: March 9, 2021. CI - (c) 2026. The Author(s). FAU - Fiedler, Goeran AU - Fiedler G AUID- ORCID: 0000-0003-1532-1248 AD - Department of Rehabilitation Science and Technology, University of Pittsburgh School of Health and Rehabilitation Sciences, 6425 Penn Ave, Suite 403-2, Pittsburgh, PA, 15206, USA. gfiedler@pitt.edu. FAU - Gale, Tom AU - Gale T AD - Department of Orthopedic Surgery, University of Pittsburgh School of Medicine, 3820 South Water Street, Pittsburgh, PA, 15206, USA. FAU - Paulus, Paige AU - Paulus P AD - Department of Orthopedic Surgery, University of Pittsburgh School of Medicine, 3820 South Water Street, Pittsburgh, PA, 15206, USA. FAU - McKernan, Gina AU - McKernan G AD - Department of Physical Medicine and Rehabilitation, University of Pittsburgh School of Medicine, 3471 Fifth Ave, Suite 910, Pittsburgh, PA, 15213, USA. AD - VA Pittsburgh Healthcare System, 6425 Penn Ave, Suite 400 #4126, Pittsburgh, PA, 15206, USA. FAU - Anderst, William AU - Anderst W AD - Department of Orthopedic Surgery, University of Pittsburgh School of Medicine, 3820 South Water Street, Pittsburgh, PA, 15206, USA. LA - eng SI - ClinicalTrials.gov/NCT05041998 GR - W81XWH2010914/U.S. Department of Defense/ GR - W81XWH2010914/U.S. Department of Defense/ GR - W81XWH2010914/U.S. Department of Defense/ GR - W81XWH2010914/U.S. Department of Defense/ GR - W81XWH2010914/U.S. Department of Defense/ PT - Journal Article PT - Randomized Controlled Trial DEP - 20260803 PL - England TA - BMC Musculoskelet Disord JT - BMC musculoskeletal disorders JID - 100968565 SB - IM MH - Humans MH - *Artificial Limbs MH - Cross-Over Studies MH - Female MH - *Patient Reported Outcome Measures MH - Middle Aged MH - *Prosthesis Design MH - Male MH - Biomechanical Phenomena MH - Aged MH - Gait/physiology MH - Adult MH - *Amputees/rehabilitation/psychology MH - *Amputation, Surgical/rehabilitation MH - Quality of Life MH - *Amputation Stumps/diagnostic imaging/physiopathology MH - Patient Satisfaction OTO - NOTNLM OT - Artificial limbs OT - Dynamic x-ray imaging OT - Llower limb loss OT - Prosthetic socket design COIS- Declarations. Ethics approval and consent to participate: This study adhered to the ethical principles covered in the Declaration of Helsinki. It has been approved by the Institutional Review Board at the University of Pittsburgh under ID: STUDY20070123. Written, informed consent to participate was obtained from all participants. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:53 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/03/05 00:00 [received] PHST- 2026/07/29 00:00 [accepted] PHST- 2026/10/30 00:53 [entrez] AID - 10.1186/s12891-026-10313-1 [pii] AID - 10.1186/s12891-026-10313-1 [doi] PST - epublish SO - BMC Musculoskelet Disord. 2026 Aug 3;27(1):932. doi: 10.1186/s12891-026-10313-1. PMID- 42958200 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2482 (Electronic) IS - 1471-2482 (Linking) VI - 26 IP - 1 DP - 2026 Aug 4 TI - Delayed spinal endoscopy-assisted posterolateral removal of deep retained glass fragments at the pediatric cervicothoracic junction after emergency debridement: a case report. LID - 730 [pii] LID - 10.1186/s12893-026-04042-4 [doi] AB - BACKGROUND: Deep retained glass foreign bodies at the pediatric cervicothoracic junction are rare and technically challenging. This region is close to the neural foramen, nerve roots, pleural apex, deep vessels, and osseous structures. During the contaminated emergency phase, aggressive exploration in pursuit of complete radiological clearance may increase the risk of iatrogenic injury. A staged strategy may therefore be considered when selected high-risk fragments cannot be safely removed during initial debridement. CASE PRESENTATION: We report the case of a 13-year-old boy who sustained an open glass injury to the left side of the neck in a traffic accident. Initial computed tomography (CT) showed multiple hyperdense foreign bodies in the left posterolateral neck and cervicothoracic soft tissues. The deeper fragments were adjacent to the left second costotransverse region and in close proximity to the left T1-T2 intervertebral foramen. Cervical computed tomography angiography (CTA) and venography (CTV) identified no major vascular injury. Emergency debridement and exploration were performed through the original wounds, and several accessible large glass fragments were removed. Deep fragments that could not be safely accessed were not pursued by blind or extended exploration. Three months later, follow-up CT showed persistent, relatively large retained glass fragments adjacent to the left second costotransverse region. After detailed imaging assessment and preoperative localization, delayed removal was performed using a Delta spinal endoscopic system through a posterolateral working channel established via the left T1-T2 intertransverse space. Two large deep glass fragments and multiple irregular fragments were removed under magnified endoscopic visualization. Postoperative CT confirmed removal of the target deep foreign bodies, with only scattered tiny hyperdense foci remaining. The child recovered uneventfully, with good wound healing and normal sensation and muscle strength in both upper limbs. CONCLUSION: This case illustrates that, after emergency debridement, delayed Delta spinal endoscopy-assisted posterolateral removal may be considered for selected deeply retained glass fragments at the pediatric cervicothoracic junction when repeat imaging confirms stable localization and a safe working corridor. Small, scattered, asymptomatic residual fragments may be managed with close follow-up. CI - (c) 2026. The Author(s). FAU - Wei, Mingyang AU - Wei M AD - First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region, China. FAU - Shi, Jiantao AU - Shi J AD - First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region, China. FAU - Dai, Yi AU - Dai Y AD - First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region, China. FAU - Jiao, Lei AU - Jiao L AD - First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region, China. FAU - Yang, Xinyumeng AU - Yang X AD - First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region, China. FAU - Sun, Jianhua AU - Sun J AD - First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang Uygur Autonomous Region, China. 007sunjianhua@sina.com. LA - eng GR - Project No.: 2023CB008-33;Project No.: CZ001519;Project No.: 2024DA047/Xinjiang Production and Construction Corps/ PT - Case Reports PT - Journal Article DEP - 20260804 PL - England TA - BMC Surg JT - BMC surgery JID - 100968567 SB - IM MH - Humans MH - Male MH - Adolescent MH - *Debridement MH - *Cervical Vertebrae/surgery/injuries/diagnostic imaging MH - *Foreign Bodies/surgery/diagnostic imaging MH - *Endoscopy/methods MH - *Glass MH - *Thoracic Vertebrae/surgery/injuries/diagnostic imaging MH - *Neck Injuries/surgery/diagnostic imaging MH - Accidents, Traffic MH - Tomography, X-Ray Computed OTO - NOTNLM OT - Case report OT - Cervicothoracic junction OT - Delta spinal endoscopy OT - Glass foreign body OT - Pediatric trauma OT - Penetrating neck injury COIS- Declarations. Ethics approval and consent to participate: The requirement for ethics approval was waived by the Ethics Committee of the First Affiliated Hospital of Shihezi University because this manuscript is a case report. Written informed consent for publication was obtained from the patient's legal guardian. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:53 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/20 00:00 [received] PHST- 2026/07/10 00:00 [accepted] PHST- 2026/10/30 00:53 [entrez] AID - 10.1186/s12893-026-04042-4 [pii] AID - 10.1186/s12893-026-04042-4 [doi] PST - epublish SO - BMC Surg. 2026 Aug 4;26(1):730. doi: 10.1186/s12893-026-04042-4. PMID- 42958198 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1472-6831 (Electronic) IS - 1472-6831 (Linking) VI - 26 IP - 1 DP - 2026 Aug 4 TI - Soft tissue prediction: conditional generative adversarial network versus Dolphin visual treatment objective in Asian adult class I extraction therapy. LID - 2144 [pii] LID - 10.1186/s12903-026-09378-0 [doi] AB - BACKGROUND: This study compared the accuracy and clinical applicability of a deep learning-based artificial intelligence (AI) model and the Dolphin visual treatment objective (VTO) to predict soft tissue changes after premolar extraction in patients with skeletal Class I malocclusion to provide empirical evidence for guiding prediction tool selection. METHODS: Thirty adult patients with skeletal Class I malocclusion who underwent extraction orthodontic treatment were enrolled. Pre-treatment (T1) and post-treatment (T2) lateral cephalometric images were collected. A conditional generative adversarial network (CGAN) model modified with a U-Net generator and PatchGAN discriminator was constructed to predict post-treatment soft tissue morphology. The AI model was pre-trained using cephalometric data from 511 patients. Tooth movement vectors from T1 to T2 served as model inputs. Data augmentation, early stopping, L2 regularization, and dropout layers were applied to mitigate overfitting. After training, 30 patients were included as an independent test set. Dolphin VTO served as the conventional comparison method. Soft tissue cephalometric analysis was conducted on AI-predicted, Dolphin VTO-predicted, and actual T2 images to measure landmark 2D Euclidean distances and soft tissue cephalometric indices. For qualitative validation, 15 random cases were evaluated by four calibrated raters (blinded to the prediction methods) using a 100-mm visual analog scale (VAS) to assess the similarity of predicted and actual facial profiles. Two-way multivariate analysis of variance (MANOVA) was used to compare VAS scores. RESULTS: Intra- and inter-investigator reliabilities for landmark identification showed good consistency. For each case, the CGAN model achieved an inference time of approximately 2 s, whereas Dolphin VTO required approximately 5-10 min of manual operation. Compared with Dolphin VTO, the AI model produced significantly lower total mean landmark prediction error and had smaller prediction deviations from actual values for all soft tissue cephalometric indices. MANOVA revealed significant between-method differences in perceptual ratings. The AI model showed significantly higher VAS scores for profile, upper lip, and lower lip predictions, but without a significant difference in subnasal and chin region ratings. Rater's professional background had no significant interactive effect on the evaluation. CONCLUSIONS: The CGAN-based AI model outperformed Dolphin VTO in predicting soft tissue changes and facial profile esthetics after extraction treatment of skeletal Class I malocclusion, with clinically acceptable prediction errors. This AI model may serve as an effective tool for predicting post-orthodontic treatment facial esthetics, facilitating clinical treatment planning, and doctor-patient communication. CI - (c) 2026. The Author(s). FAU - Gong, Beiwen AU - Gong B AD - Department of Orthodontics, School of Stomatology, Capital Medical University, No.9 Fanjiacun Road, Fengtai District, Beijing, 100070, China. FAU - Chang, Qiao AU - Chang Q AD - Department of Orthodontics, School of Stomatology, Capital Medical University, No.9 Fanjiacun Road, Fengtai District, Beijing, 100070, China. FAU - Shi, Tianlei AU - Shi T AD - LargeV Instrument Corp., Ltd, Beijing, 100192, China. FAU - Zhang, Tong AU - Zhang T AD - Department of Orthodontics, School of Stomatology, Capital Medical University, No.9 Fanjiacun Road, Fengtai District, Beijing, 100070, China. FAU - Wang, Yajie AU - Wang Y AD - LargeV Instrument Corp., Ltd, Beijing, 100192, China. AD - Department of Engineering Physics, Tsinghua University, Beijing, 100084, China. FAU - Zuo, Feifei AU - Zuo F AD - LargeV Instrument Corp., Ltd, Beijing, 100192, China. FAU - Fang, Dongyu AU - Fang D AD - Department of Orthodontics, School of Stomatology, Capital Medical University, No.9 Fanjiacun Road, Fengtai District, Beijing, 100070, China. FAU - Xie, Xianju AU - Xie X AD - Department of Orthodontics, School of Stomatology, Capital Medical University, No.9 Fanjiacun Road, Fengtai District, Beijing, 100070, China. dentist.x@163.com. FAU - Bai, Yuxing AU - Bai Y AD - Department of Orthodontics, School of Stomatology, Capital Medical University, No.9 Fanjiacun Road, Fengtai District, Beijing, 100070, China. byuxing@ccmu.edu.cn. LA - eng GR - YSP202509/Beijing Stomatological Hospital, Capital Medical University Young Scientist Program/ GR - 2024YFE0107100/National Key Research and Development Program for Government-to-Government International Scientific and Technological Cooperation/ PT - Comparative Study PT - Journal Article DEP - 20260804 PL - England TA - BMC Oral Health JT - BMC oral health JID - 101088684 SB - IM MH - Humans MH - Generative Adversarial Networks MH - Cephalometry MH - *Tooth Extraction MH - Female MH - Male MH - *Face/anatomy & histology MH - Generative Artificial Intelligence MH - Adult MH - Bicuspid/surgery OTO - NOTNLM OT - Conditional generative adversarial network OT - Dolphin VTO OT - Extraction treatment OT - Facial esthetics OT - Orthodontics OT - Skeletal class I malocclusion OT - Soft tissue prediction COIS- Declarations. Ethics approval and consent to participate: This study was approved by the Institutional Review Board of Beijing Stomatological Hospital, Capital Medical University (approval number: CMUSH-IRB-KJ-PJ-2024-72). Written informed consent was obtained from all participants. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:53 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/28 00:00 [received] PHST- 2026/07/17 00:00 [accepted] PHST- 2026/10/30 00:53 [entrez] AID - 10.1186/s12903-026-09378-0 [pii] AID - 10.1186/s12903-026-09378-0 [doi] PST - epublish SO - BMC Oral Health. 2026 Aug 4;26(1):2144. doi: 10.1186/s12903-026-09378-0. PMID- 42958186 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1472-6831 (Electronic) IS - 1472-6831 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Factors associated with prolonged hospital stay in mandibular condylar fractures: a multicenter retrospective study in Indonesia. LID - 2136 [pii] LID - 10.1186/s12903-026-09511-z [doi] AB - BACKGROUND: Mandibular condylar fractures constitute a significant proportion of maxillofacial trauma and are associated with functional impairment and increased healthcare utilization. However, factors associated with the duration of hospital stay remain insufficiently understood, particularly in low- and middle-income countries (LMICs). This study aimed to characterize the epidemiology of mandibular condylar fractures and identify factors associated with prolonged hospital stay. METHODS: A retrospective multicenter study was conducted across three tertiary referral centers in Indonesia from January 2020 to May 2025. A total of 178 patients with confirmed mandibular condylar fractures were included. Variables analyzed included demographic characteristics, mechanism of injury, fracture characteristics, presence of associated mandibular fractures, and treatment modalities. Length of hospital stay (LoS) was analyzed as a dichotomous outcome (< 7 vs. >/= 7 days), with the 7-day threshold selected based on the cohort median and clinical interpretability. Bivariate analyses were followed by multivariable logistic regression. Sensitivity analysis was performed using negative binomial regression with continuous LoS. RESULTS: Road traffic accidents (80.9%) were the predominant mechanism of injury, and 72.5% of patients presented with multiple mandibular fractures. In multivariable analysis, road traffic accidents (OR = 2.715; 95% CI = 1.131-6.515; p = 0.025) and the presence of multiple mandibular fractures (OR = 3.464; 95% CI = 1.710-7.016; p < 0.001) were significantly associated with prolonged hospital stay. In contrast, age, gender, and treatment modality were not significantly associated with LoS. CONCLUSIONS: Condylar fracture patients involved in road traffic accidents or those presenting with multiple mandibular fractures were more likely to experience prolonged hospital stay. These findings suggest that injury-related characteristics may be more closely associated with hospitalization outcomes than fracture-specific characteristics within this cohort. However, the observed associations should be interpreted cautiously given the retrospective design, the absence of standardized trauma severity measures, and substantial missing data for several groups. Early identification of patients presenting with these characteristics may support risk stratification and optimize resource allocation in resource-limited settings. CI - (c) 2026. The Author(s). FAU - Rahimah, Aulia S AU - Rahimah AS AD - Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia. FAU - Julia, Vera AU - Julia V AD - Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia. vera.julia@ui.ac.id. AD - Dental Department, Universitas Indonesia Hospital, Depok, 16424, Indonesia. vera.julia@ui.ac.id. FAU - Iskandar, Faradiba N R AU - Iskandar FNR AD - Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia. FAU - Wijaya, Arbi AU - Wijaya A AD - Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia. AD - Division of Oral and Maxillofacial Oncology and Surgical Sciences, Graduate School of Dentistry, Tohoku University, Sendai, Miyagi, 980-0872, Japan. FAU - Ratman, M Farid AU - Ratman MF AD - Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia. AD - Dental Department, Universitas Indonesia Hospital, Depok, 16424, Indonesia. FAU - Latief, M Adhitya AU - Latief MA AD - Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia. FAU - Sulistyani, Lilies D AU - Sulistyani LD AD - Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia. FAU - Nakamura, Norifumi AU - Nakamura N AD - Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Indonesia, Jakarta, 10430, Indonesia. LA - eng PT - Journal Article PT - Multicenter Study DEP - 20260803 PL - England TA - BMC Oral Health JT - BMC oral health JID - 101088684 SB - IM MH - Humans MH - Retrospective Studies MH - *Mandibular Fractures/epidemiology/therapy/etiology MH - Indonesia/epidemiology MH - Female MH - *Length of Stay/statistics & numerical data MH - *Mandibular Condyle/injuries MH - Male MH - Adult MH - Accidents, Traffic/statistics & numerical data MH - Adolescent MH - Risk Factors MH - Middle Aged MH - Young Adult OTO - NOTNLM OT - Developing countries OT - Injury patterns OT - Length of stay OT - Maxillofacial injuries OT - Traffic accidents OT - Treatment patterns COIS- Declarations. Ethics approval and consent to participate: The study was conducted in accordance with the Declaration of Helsinki and approved by the Dental Research Ethics Committee of the Faculty of Dentistry, University of Indonesia (Protocol No. 010770725), the Ethics Committee of the Faculty of Medicine, University of Indonesia (Approval No. 25071035), and the Ethics Committee of Universitas Indonesia Hospital (Protocol No. 202507140). As this was a retrospective study using existing medical records with no direct patient contact or intervention, the requirement for informed consent to participate was waived by the approving Institutional Review Boards. All patient data were anonymized prior to analysis. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:52 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/11 00:00 [received] PHST- 2026/07/30 00:00 [accepted] PHST- 2026/10/30 00:52 [entrez] AID - 10.1186/s12903-026-09511-z [pii] AID - 10.1186/s12903-026-09511-z [doi] PST - epublish SO - BMC Oral Health. 2026 Aug 3;26(1):2136. doi: 10.1186/s12903-026-09511-z. PMID- 42958184 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2482 (Electronic) IS - 1471-2482 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Development and internal validation of a perioperative nomogram for predicting moderate-to-severe acute postoperative pain after hepatectomy for hepatocellular carcinoma. LID - 728 [pii] LID - 10.1186/s12893-026-04075-9 [doi] AB - BACKGROUND: Moderate-to-severe acute postoperative pain (APOP) after hepatectomy for hepatocellular carcinoma (HCC) may impede early postoperative recovery. However, predictive tools integrating preoperative and intraoperative risk factors are lacking. This study aimed to develop and internally validate a perioperative nomogram for predicting APOP in this population. METHODS: Adult patients who underwent elective hepatectomy for primary HCC between January 2021 and January 2025 were retrospectively enrolled and randomly divided into a training cohort (n = 301) and a holdout internal validation cohort (n = 129). Moderate-to-severe APOP was defined as a maximum prerescue NRS score >/= 4 during repeated pain assessments within 72 h after surgery. Candidate predictors included demographic, tumor-related, laboratory, psychological, and intraoperative variables. Multiple imputation was used to address missing data, and LASSO followed by multivariable logistic regression was used to select predictors and construct the perioperative nomogram. Model performance was assessed using the area under the curve (AUC), calibration curves, and decision curve analysis (DCA). RESULTS: Moderate-severe APOP occurred in 42.1% of patients. Seven predictors were included: younger age, an actual open surgical approach, a larger maximal tumor diameter, a higher neutrophil-to-lymphocyte ratio, greater intraoperative opioid consumption, and higher preoperative Hospital Anxiety and Depression Scale and Pain Catastrophizing Scale scores. The nomogram showed good discrimination in the training cohort (AUC = 0.818; 95% CI: 0.771-0.865) and the holdout internal validation cohort (AUC = 0.795; 95% CI: 0.720-0.870), with satisfactory calibration and positive net clinical benefit in terms of DCA. CONCLUSION: A perioperative nomogram integrating preoperative and intraoperative variables was developed and internally validated. Because the complete predictor set becomes available only at the end of surgery, the model is intended to support immediate postoperative risk stratification and individualized analgesic and psychological interventions after hepatectomy for patients with HCC. CI - (c) 2026. The Author(s). FAU - Wang, Juan AU - Wang J AD - Department of General Surgery (Vascular Ward), The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu Province, 221000, China. FAU - Fan, Chunmei AU - Fan C AD - Oncology Department, Donghai County People's Hospital, Lianyungang, Jiangsu Province, 222300, China. FAU - Zhang, Wanqiu AU - Zhang W AD - Department of General Surgery (Vascular Ward), The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu Province, 221000, China. FAU - Zhang, Yan AU - Zhang Y AD - Department of General Surgery (Vascular Ward), The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu Province, 221000, China. FAU - Li, Ting AU - Li T AD - Department of General Surgery (Vascular Ward), The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu Province, 221000, China. 18361268580@163.com. AD - , No.9 Kunpeng Road, Xuzhou, Jiangsu Province, 221000, China. 18361268580@163.com. LA - eng PT - Journal Article PT - Validation Study DEP - 20260803 PL - England TA - BMC Surg JT - BMC surgery JID - 100968567 SB - IM MH - Humans MH - *Carcinoma, Hepatocellular/surgery MH - *Nomograms MH - *Liver Neoplasms/surgery MH - *Hepatectomy/adverse effects MH - Female MH - *Postoperative Pain/diagnosis/etiology MH - Male MH - Retrospective Studies MH - Middle Aged MH - Aged MH - Risk Factors MH - Pain Measurement MH - *Acute Pain/diagnosis/etiology OTO - NOTNLM OT - Acute postoperative pain OT - Hepatectomy OT - Hepatocellular carcinoma OT - Nomogram OT - Risk prediction COIS- Declarations. Ethics approval and consent to participate: This single-center retrospective cohort study was conducted to develop and internally validate a prediction model for moderate-to-severe APOP in patients undergoing hepatectomy for HCC. The study protocol was reviewed and approved by the Institutional Review Board of the participating institution (IRB approval No. XYFY2026-KL173-01). Owing to the retrospective design and the use of routinely collected clinical data, the requirement for individual written informed consent was waived. All data were de-identified before analysis. All methods were carried out in accordance with Declaration of Helsinki. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:52 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/24 00:00 [received] PHST- 2026/07/21 00:00 [accepted] PHST- 2026/10/30 00:52 [entrez] AID - 10.1186/s12893-026-04075-9 [pii] AID - 10.1186/s12893-026-04075-9 [doi] PST - epublish SO - BMC Surg. 2026 Aug 3;26(1):728. doi: 10.1186/s12893-026-04075-9. PMID- 42958178 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1472-6831 (Electronic) IS - 1472-6831 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Clinical outcomes and panoramic fractal analysis of trabecular bone changes in intrabony defects treated with the entire papilla preservation technique: a retrospective longitudinal study. LID - 2133 [pii] LID - 10.1186/s12903-026-09485-y [doi] AB - BACKGROUND: Clinical periodontal parameters are widely used to evaluate treatment outcomes in intrabony defects, but they do not directly reflect post-treatment radiographic trabecular texture changes. This retrospective longitudinal study aimed to evaluate clinical periodontal outcomes and radiographic trabecular texture changes using panoramic fractal analysis in intrabony defects treated with the entire papilla preservation technique. METHODS: This retrospective longitudinal study included patients with intrabony defects treated with the entire papilla preservation technique who had complete clinical records and panoramic radiographs at baseline, 6 months, and 1 year. Only biomaterial-free cases with isolated two- or three-wall intrabony defects were included. Fractal dimension (FD) values were calculated using ImageJ from standardized defect-side and reference regions of interest. Clinical periodontal parameters and FD values were compared over time using Friedman and Wilcoxon signed-rank tests, and exploratory associations between radiographic and clinical changes were assessed using Spearman correlation analysis. RESULTS: The median averaged defect-site FD increased significantly from 1.089 at baseline to 1.186 at 6 months and 1.240 at 1 year (p < 0.001; Kendall's W = 0.909). All pairwise comparisons were significant after Bonferroni correction (p < 0.001). In contrast, reference region FD values did not change significantly (p = 0.417). Probing depth and clinical attachment loss also improved significantly over time (both p < 0.001; Kendall's W = 0.935), whereas gingival recession remained stable. No significant correlations were found between changes in averaged defect-site FD and changes in clinical periodontal parameters. CONCLUSIONS: Within the limitations of this retrospective study, intrabony defects treated with an entire papilla preservation technique-based minimally invasive approach showed significant clinical improvement accompanied by an increase in defect-site FD values. Panoramic fractal analysis may provide complementary, non-invasive information on radiographic trabecular texture changes compatible with local trabecular reorganization after treatment, but should be considered an adjunct to clinical periodontal assessment rather than a substitute. TRIAL REGISTRATION: Not applicable. This was a retrospective observational study and did not require trial registration. CI - (c) 2026. The Author(s). FAU - Keles, Mert AU - Keles M AD - Department of Periodontology, Dental Clinic Academy, Ankara, Turkiye, 06510, Turkey. mertkeles05@gmail.com. FAU - Yarkac, Fatma Ucan AU - Yarkac FU AD - Department of Periodontology, Faculty of Dentistry, Necmettin Erbakan University, Konya, Turkiye, Turkey. FAU - Ogutcen, Omer AU - Ogutcen O AD - Department of Periodontology, Faculty of Dentistry, Necmettin Erbakan University, Konya, Turkiye, Turkey. FAU - Yuksel, Ibrahim Burak AU - Yuksel IB AD - Department of Oral and Maxillofacial Radiology, Faculty of Dentistry, Necmettin Erbakan University, Konya, Turkiye, Turkey. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - BMC Oral Health JT - BMC oral health JID - 101088684 SB - IM MH - Humans MH - Retrospective Studies MH - Longitudinal Studies MH - *Fractals MH - *Radiography, Panoramic MH - *Alveolar Bone Loss/diagnostic imaging/surgery MH - Female MH - Treatment Outcome MH - Male MH - Middle Aged MH - *Cancellous Bone/diagnostic imaging OTO - NOTNLM OT - Entire papilla preservation technique OT - Fractal analysis OT - Intrabony defect OT - Panoramic radiography OT - Periodontal surgery OT - Trabecular bone COIS- Declarations. Ethics approval and consent to participate: This retrospective longitudinal observational study was designed and reported in accordance with the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) statement. The study was approved by the Ethics Committee for Non-Drug and Non-Medical Device Scientific Research of Necmettin Erbakan University Faculty of Dentistry (Decision No: 2025/655; Date: September 25, 2025) and was conducted in accordance with the Declaration of Helsinki. Because anonymized retrospective clinical and radiographic records were used, the requirement for informed consent to participate was waived by the ethics committee. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:52 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/29 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/30 00:52 [entrez] AID - 10.1186/s12903-026-09485-y [pii] AID - 10.1186/s12903-026-09485-y [doi] PST - epublish SO - BMC Oral Health. 2026 Aug 3;26(1):2133. doi: 10.1186/s12903-026-09485-y. PMID- 42958177 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2458 (Electronic) IS - 1471-2458 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Evaluation of Iraq's national plan for hepatitis B control: identifying gaps and strategies for improvement. LID - 3113 [pii] LID - 10.1186/s12889-026-28782-3 [doi] AB - BACKGROUND: Hepatitis B virus (HBV) remains a significant public health challenge in Iraq, necessitating a comprehensive evaluation of the national hepatitis B control plan. This study aimed to assess the epidemiological trends of HBV in Iraq from 2015 to 2022 and evaluate the implementation and effectiveness of the national control plan. METHODS: A descriptive cross-sectional study was conducted using national HBV surveillance data from all available Iraqi governorates between 2015 and 2022. The program evaluation component was performed in Wasit Governorate and included 24 randomly selected primary health care centers (PHCCs). Descriptive statistics, Mann-Whitney U, Kruskal-Wallis, and chi-square tests were performed using SPSS version 25 and Microsoft Excel. RESULTS: The annual HBV incidence rate declined from 8.3 to 3.5 per 100,000 population between 2015 and 2022, with consistently higher incidence rates among males and marked geographic variation across governorates. The highest incidence rates were observed among individuals aged 15-45 years and those aged > 45 years. Childhood hepatitis B vaccination coverage exceeded the national target by 2022; however, prevention of mother-to-child transmission and HBV diagnosis coverage remained below target levels. Evaluation of PHCC performance showed that 88% achieved acceptable evaluation scores, although substantial variability in performance was observed across facilities. CONCLUSION: This study provides a comprehensive evaluation of Iraq's national hepatitis B control plan, demonstrating declining incidence rates alongside important demographic and geographic disparities. Although vaccination coverage has improved substantially, important gaps remain in HBV diagnosis, prevention of mother-to-child transmission, and PHCC performance. These findings provide evidence to guide targeted interventions, strengthen health service delivery, and inform future policy decisions aimed at advancing hepatitis B control in Iraq. CI - (c) 2026. The Author(s). FAU - Jalal, Mohammed A AU - Jalal MA AUID- ORCID: 0009-0006-2206-8059 AD - Department of Epidemiology, School of Public Health & Safety, Shahid Beheshti University of Medical Sciences, Tehran, Iran. m.health81@gmail.com. AD - Epidemiology Section, Department of Public Health, Directorate of Wasit Health, Ministry of Health, Wasit, Iraq. m.health81@gmail.com. FAU - Karami, Manoochehr AU - Karami M AD - Department of Epidemiology, School of Public Health & Safety, Shahid Beheshti University of Medical Sciences, Tehran, Iran. AD - Pandemic and Epidemic Preparedness and Response Research Network, Research Institute for Health Sciences and Environment, Shahid Beheshti University of Medical Sciences, Tehran, Iran. FAU - Namdari, Mahshid AU - Namdari M AD - Department of Epidemiology, School of Public Health & Safety, Shahid Beheshti University of Medical Sciences, Tehran, Iran. FAU - Lami, Faris AU - Lami F AD - Department of Family and Community Medicine, College of Medicine in Baghdad University, Baghdad, Iraq. FAU - Taher, Taqi Mohammed Jwad AU - Taher TMJ AD - Department of Family and Community Medicine, College of Medicine in Wasit University, Wasit, Iraq. FAU - Etemad, Koorosh AU - Etemad K AD - Department of Epidemiology, School of Public Health & Safety, Shahid Beheshti University of Medical Sciences, Tehran, Iran. etemadk@gmail.com. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - BMC Public Health JT - BMC public health JID - 100968562 RN - 0 (Hepatitis B Vaccines) SB - IM MH - Humans MH - Iraq/epidemiology MH - Cross-Sectional Studies MH - Adolescent MH - *Hepatitis B/epidemiology/prevention & control MH - Adult MH - Female MH - Male MH - Middle Aged MH - Young Adult MH - Incidence MH - Program Evaluation MH - Child MH - Child, Preschool MH - Hepatitis B Vaccines/administration & dosage MH - Infant OTO - NOTNLM OT - Epidemiology OT - Hepatitis B virus OT - Iraq OT - National control plan OT - Program evaluation COIS- Declarations. Ethics approval and consent to participate: The study protocol was approved by the ethics review board of Shahid Beheshti University of Medical Sciences (Approval ID: IR.SBMU.PHNS.REC.1404.044). Moreover. Research Committee in the National Center for Training and Human Development Iraq Ministry of Health (MOH) by a formal letter according to the (Approval ID: IRAQ.MOH.FORM NUMBER 04/2012. DECISION NUMBER:24). We have obtained approvals from the relevant institutions, as described earlier, to collect data that had been previously collected. Our evaluation focused on the program's process, without involving any application (no applicable) to humans or human tissues. Consent for publication: Not applicable. Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:52 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2024/04/30 00:00 [received] PHST- 2026/07/24 00:00 [accepted] PHST- 2026/10/30 00:52 [entrez] AID - 10.1186/s12889-026-28782-3 [pii] AID - 10.1186/s12889-026-28782-3 [doi] PST - epublish SO - BMC Public Health. 2026 Aug 3;26(1):3113. doi: 10.1186/s12889-026-28782-3. PMID- 42958171 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2458 (Electronic) IS - 1471-2458 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Prevalence and factors associated with social media addiction among undergraduate students from selected districts of Kerala, India: a cross-sectional study. LID - 3126 [pii] LID - 10.1186/s12889-026-28841-9 [doi] AB - BACKGROUND: Although evidence on Social Media Addiction (SMA) is growing globally, research from India remains limited. This study aimed to estimate the prevalence of SMA and its associated factors among undergraduate students from selected districts of Kerala, India. METHODS: This cross-sectional study included 823 Arts & Science and Engineering college students (mean age 19.8 years, 60% female) from two districts in Kerala, selected through a multistage cluster sampling design. The Bergen Social Media Addiction Scale (BSMAS) was used to assess SMA. Survey-weighted generalized linear models with a Poisson family and log link were used to identify factors associated with SMA. RESULTS: The survey-weighted prevalence of SMA was 38.07% (95% CI: 33.56-42.81), while the prevalence of social media disorder (SMD) was 4.03% (95% CI: 2.69-5.99). Among participants with SMA, 12.67% met the criteria for SMD. In the adjusted analysis, female students had a higher prevalence of SMA than males (APR = 1.42; 95% CI: 1.07-1.87; p = 0.018). Married students had a higher prevalence of SMA than unmarried students (APR = 1.73; 95% CI: 1.33-2.24; p < 0.001), although the number of married participants was small. Students who used social media for more than 5 h per day had a higher prevalence of SMA than those using it for 0-5 h per day (APR = 1.41; 95% CI: 1.12-1.78; p = 0.006). A weak positive correlation was observed between daily social media duration and BSMAS score (Spearman's rho = 0.183, p < 0.001). CONCLUSION: In this study of 823 undergraduate students, more than one-third were affected by SMA, with a notable proportion experiencing SMD. These findings highlight the need for targeted digital well-being interventions in college settings that focus on reducing excessive screen time and promoting responsible social media use. CI - (c) 2026. The Author(s). FAU - Krishna, Swathy AU - Krishna S AD - Department of Public Health, Amrita School of Medicine, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India. AD - Department of Community Medicine and School of Public Health, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh, India. FAU - Olickal, Jeby Jose AU - Olickal JJ AD - Department of Public Health, Amrita School of Medicine, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India. jebyjose@aims.amrita.edu. FAU - Thankappan, Kavumpurathu Raman AU - Thankappan KR AD - Department of Public Health, Amrita School of Medicine, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - BMC Public Health JT - BMC public health JID - 100968562 SB - IM MH - Humans MH - Female MH - India/epidemiology MH - Cross-Sectional Studies MH - *Social Media/statistics & numerical data MH - *Students/statistics & numerical data/psychology MH - Male MH - Prevalence MH - Young Adult MH - Universities MH - *Behavior, Addictive/epidemiology MH - Adolescent MH - Risk Factors MH - Digital Media MH - Surveys and Questionnaires OTO - NOTNLM OT - Behavioural Addiction OT - Social Media Addiction OT - Young Adults COIS- Declarations. Ethics approval and consent to participate: This study was conducted in accordance with the Declaration of Helsinki and was approved by the Institutional Review Board of the Institutional Ethics Committee, Amrita School of Medicine, Kochi (Approval number: ECASM-AIMS-2024-067). Written informed consent was obtained from all participants prior to participation. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:52 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/18 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/30 00:52 [entrez] AID - 10.1186/s12889-026-28841-9 [pii] AID - 10.1186/s12889-026-28841-9 [doi] PST - epublish SO - BMC Public Health. 2026 Aug 3;26(1):3126. doi: 10.1186/s12889-026-28841-9. PMID- 42958170 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1472-6831 (Electronic) IS - 1472-6831 (Linking) VI - 26 IP - 1 DP - 2026 Aug 1 TI - Efficiency of laser-activated and multisonic irrigation systems in removing calcium hydroxide from simulated internal root resorption defects: an in vitro study. LID - 2130 [pii] LID - 10.1186/s12903-026-09295-2 [doi] AB - BACKGROUND: Complete removal of calcium hydroxide (CH) from resorption cavities remains challenging and requires advanced irrigation techniques beyond syringe-needle irrigation (SNI). This study aimed to compare the efficiency of Er: YAG and Er, Cr: YSGG laser-activated irrigation with multisonic high-speed irrigation using the GentleWave G4 ProControl (GW) in removing CH from standardized internal root resorption cavities, versus SNI. METHODS: Transparent 3D-printed models with spherical internal resorption cavities (1.6 mm in diameter, located 5 mm from the apex) were filled with CH, and randomly allocated into four groups (n = 20): SNI, Er: YAG, Er, Cr: YSGG, and GW. The time for complete visual CH removal was recorded and analyzed (P < .05). RESULTS: GW significantly showed the shortest CH removal time (12.55 +/- 1.34 s), followed by Er: YAG (48.02 +/- 4.69 s) and Er, Cr: YSGG (46.65 +/- 4.41 s), with no significant difference between the laser systems (P > .05). SNI resulted in the longest removal time (141.60 +/- 13.32 s) (P < .001). CONCLUSIONS: Within the limitations of this in vitro study, the GentleWave G4 ProControl was the most effective method, and the laser systems presented promising results compared to SNI. Enhanced fluid dynamics from advanced systems may help reduce chair time; however, given the in vitro design, their potential to support minimally invasive approaches in pediatric endodontics requires confirmation in clinical investigations. CI - (c) 2026. The Author(s). FAU - Ozyurek, Hande AU - Ozyurek H AUID- ORCID: 0000-0001-9670-953X AD - Department of Pediatric Dentistry, Faculty of Dentistry, Kocaeli Health and Technology University, Kocaeli, 41030, Turkiye. handeozyurek@hotmail.com. AD - Department of Pediatric, Faculty of Dentistry, Kocaeli Health and Technology University, Kocaeli, 41275, Turkiye. handeozyurek@hotmail.com. FAU - Ozdemir, Olcay AU - Ozdemir O AUID- ORCID: 0000-0001-8867-1551 AD - Department of Endodontics, Faculty of Dentistry, Karabuk University, Karabuk, 78050, Turkiye. FAU - Aminimofrad, Ali AU - Aminimofrad A AUID- ORCID: 0009-0002-8758-2463 AD - Department of Endodontics, Faculty of Dentistry, Bahcesehir University, Istanbul, 34357, Turkiye. FAU - Gundogar, Mustafa AU - Gundogar M AUID- ORCID: 0000-0001-8656-7101 AD - Department of Endodontics, Faculty of Dentistry, Istanbul Medipol University, Istanbul, 34214, Turkiye. FAU - Ozyurek, Taha AU - Ozyurek T AUID- ORCID: 0000-0003-3299-3361 AD - Department of Endodontics, Faculty of Dentistry, Bahcesehir University, Istanbul, 34357, Turkiye. LA - eng PT - Comparative Study PT - Journal Article DEP - 20260801 PL - England TA - BMC Oral Health JT - BMC oral health JID - 101088684 RN - PF5DZW74VN (Calcium Hydroxide) RN - 0 (Root Canal Irrigants) SB - IM MH - *Root Resorption/therapy MH - *Calcium Hydroxide MH - *Therapeutic Irrigation/methods/instrumentation MH - Humans MH - *Lasers, Solid-State/therapeutic use MH - *Root Canal Irrigants MH - In Vitro Techniques OTO - NOTNLM OT - Calcium hydroxide removal OT - GentleWave system OT - Internal root resorption OT - Intracanal medicament removal OT - Irrigation activation OT - Laser-activated irrigation OT - Pediatric endodontics COIS- Declarations. Ethics approval and consent to participate: Ethical approval is not applicable because this article contains no studies on human or animal subjects. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:52 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/02 00:00 [received] PHST- 2026/07/10 00:00 [accepted] PHST- 2026/10/30 00:52 [entrez] AID - 10.1186/s12903-026-09295-2 [pii] AID - 10.1186/s12903-026-09295-2 [doi] PST - epublish SO - BMC Oral Health. 2026 Aug 1;26(1):2130. doi: 10.1186/s12903-026-09295-2. PMID- 42958168 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2458 (Electronic) IS - 1471-2458 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Exploring perceptions, awareness, and healthcare-seeking behaviors related to muscle weakness among Saudi adults: a cross-sectional study. LID - 3110 [pii] LID - 10.1186/s12889-026-28692-4 [doi] AB - BACKGROUND: Muscle weakness is a common health concern that can significantly affect quality of life. Understanding public awareness, perceptions, and healthcare-seeking behaviors is important for early detection and management. This study aimed to assess awareness, perceptions, and healthcare-seeking behaviors related to muscle weakness among Saudi adults. METHODS: A cross-sectional survey was conducted among 1,000 adults in Saudi Arabia between August and October 2025. A structured questionnaire assessed knowledge of muscle weakness, perceived causes and symptoms, attitudes toward treatment, and healthcare-seeking behaviors. Descriptive statistics and chi-square tests were used to examine associations between demographic factors and awareness-related outcomes. RESULTS: A total of 1,000 participants completed the survey. Only 27.8% reported having heard of muscle weakness as a medical condition. Perceptions of susceptibility and severity as well as consequences showed considerable variability with uncertainty evident across most domains. Attitudes toward seeking medical advice and adopting preventive behaviors were mixed, with many participants expressing uncertainty or low likelihood of action. Although some respondents reported willingness to encourage others to seek care and to make lifestyle changes, a substantial proportion remained hesitant or unsure. Approximately one-third of participants reported personal or indirect experience with muscle weakness with impacts on daily life included increased dependence and frequent healthcare utilization. Statistically significant differences were observed in the distribution of responses across knowledge, perception, attitude, and experience domains (p < 0.05 in selected items). CONCLUSIONS: Among Saudi adults, there is substantial variability in perceptions, attitudes, and personal or indirect experience on muscle weakness as a medical condition. There are persistent gaps in health literacy and uncertainty regarding symptom recognition and severity appraisal or appropriate healthcare-seeking behavior. Accordingly, there is a need for targeted public health interventions to improve awareness and promote early recognition of muscle weakness through primary care services and trusted digital platforms. CI - (c) 2026. The Author(s). FAU - Ashour, Ahmed M AU - Ashour AM AUID- ORCID: 0000-0002-2118-0499 AD - Department of Pharmacology and Toxicology, College of Pharmacy, Umm Al-Qura University, Makkah, Saudi Arabia. amashour@uqu.edu.sa. AD - King Salman Center for Disability Research, Riyadh, 11614, Saudi Arabia. amashour@uqu.edu.sa. FAU - Alshahrani, Ali M AU - Alshahrani AM AUID- ORCID: 0000-0001-5460-3809 AD - King Salman Center for Disability Research, Riyadh, 11614, Saudi Arabia. AD - Department of Clinical Pharmacy, College of Pharmacy, Taif University, Taif, Saudi Arabia. FAU - Alhazmi, Bader A AU - Alhazmi BA AD - Department of Pharmacology and Toxicology, College of Pharmacy, Umm Al-Qura University, Makkah, Saudi Arabia. FAU - Alhayyan, Aliah AU - Alhayyan A AD - Third Riyadh Health Cluster, Huraymila General Hospital, Riyadh, Saudi Arabia. FAU - Alhayyan, Rawan AU - Alhayyan R AD - Family Medicine Department, Prince Sultan Military Medical City, Riyadh, Saudi Arabia. LA - eng GR - KSRG-2026-086/King Salman Center for Disability Research/ PT - Journal Article DEP - 20260803 PL - England TA - BMC Public Health JT - BMC public health JID - 100968562 SB - IM MH - Humans MH - Cross-Sectional Studies MH - Female MH - Male MH - Saudi Arabia MH - Adult MH - *Health Knowledge, Attitudes, Practice MH - *Muscle Weakness/psychology/therapy MH - *Patient Acceptance of Health Care/statistics & numerical data/psychology MH - Middle Aged MH - Young Adult MH - Adolescent MH - Surveys and Questionnaires MH - Aged OTO - NOTNLM OT - And perceptions OT - Awareness OT - Muscle weakness OT - Myasthenia gravis COIS- Declarations. Ethics approval and consent to participate: The study protocol was reviewed and approved by the Institutional Review Board at Umm Al-Qura University, approval number HAPO-02-K-012-2025-01-2444. Informed consent was obtained from all participants before data collection. Participation was voluntary, and participants were informed about the study objectives, procedures, confidentiality measures, potential risks, and their right to withdraw at any time. All data were anonymized to protect participant privacy. The study was conducted in accordance with relevant guidelines and regulations, including the principles of the Declaration of Helsinki. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:52 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/19 00:00 [received] PHST- 2026/07/20 00:00 [accepted] PHST- 2026/10/30 00:52 [entrez] AID - 10.1186/s12889-026-28692-4 [pii] AID - 10.1186/s12889-026-28692-4 [doi] PST - epublish SO - BMC Public Health. 2026 Aug 3;26(1):3110. doi: 10.1186/s12889-026-28692-4. PMID- 42958162 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2474 (Electronic) IS - 1471-2474 (Linking) VI - 27 IP - 1 DP - 2026 Aug 3 TI - Comparative analysis between pedicle screw-vertebral plate hook and temporary intersegmental pedicle screw in isthmic lumbar spondylolysis. LID - 930 [pii] LID - 10.1186/s12891-026-10135-1 [doi] AB - BACKGROUND: Although temporary intersegmental pedicle screw (TIPS) fixation improves stability of the treated segment, its isthmic fusion rate relative to that achieved with pedicle screw-vertebral plate hook (PSVPH) fixation remains unclear. Both PSVPH and TIPS are widely used in clinical practice. This study compared the clinical and radiological outcomes of PSVPH and TIPS for the treatment of lumbar spondylolysis. METHODS: A retrospective analysis was conducted on 67 patients with lumbar spondylolysis who underwent surgical treatment with PSVPH (n = 35) or TIPS (n = 32) between October 2010 and October 2021. Clinical outcomes included incision length, operative time, intraoperative blood loss, visual analog scale (VAS) score, Oswestry Disability Index (ODI), and Japanese Orthopedic Association (JOA) score. Radiographic assessments comprised range of motion, disc height of stabilized and adjacent segments, adjacent segment degeneration, and bony fusion. Three-dimensional reconstruction of lumbar computed tomography was used to evaluate fusion, and Hounsfield units of the isthmic region were measured to assess bone healing during follow-up. Independent Student's t-tests and chi-square tests were applied for intergroup comparisons. RESULTS: A total of 67 patients were included in the analysis. Incision length, operative time, and blood loss were all significantly reduced in the PSVPH group compared with the TIPS group (all P < 0.001). The 1-week postoperative VAS back pain score was significantly lower in the PSVPH group (P < 0.001). JOA scores at 3, 6, 12, and 24 months showed significantly greater improvement in the PSVPH group (P = 0.003, 0.034, < 0.001, and 0.001, respectively). At 24 months, the range of motion of the stabilized segment was significantly greater in the PSVPH group than in the TIPS group (P < 0.001). Postoperative intervertebral height of the stabilized segment in the PSVPH group at 1 week and 24 months was significantly lower than that in the TIPS group (P = 0.001; P < 0.013). Adjacent segment intervertebral height at 24 months was higher in the PSVPH group (P = 0.012), whereas adjacent segment range of motion was significantly lower (P = 0.012). The incidence of adjacent segment degeneration at 24 months was 2.9% in the PSVPH group and 25% in the TIPS group (P = 0.022). Hounsfield units in the isthmic region at 3 and 6 months postoperatively were significantly higher in the PSVPH group than in the TIPS group (both P < 0.001). The bony fusion rate at 24 months was 91.4% (32/35) in the PSVPH group and 84.3% (27/32) in the TIPS group. CONCLUSIONS: The healing rate achieved with TIPS was comparable to that of PSVPH. However, TIPS was associated with greater invasiveness, slower healing, and a higher incidence of adjacent segment complications. PSVPH demonstrated superior safety and clinical effectiveness compared with TIPS for the treatment of lumbar spondylolysis. CI - (c) 2026. The Author(s). FAU - Li, Jingyuan AU - Li J AD - Department of Traumatology and Orthopedics, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830054, China. AD - Department of Orthopaedic Surgery, 920th Hospital of Joint Logistics Support Force, Kunming, Yunnan, 650032, China. FAU - Cai, Zhijun AU - Cai Z AD - Department of Orthopaedic Surgery, 920th Hospital of Joint Logistics Support Force, Kunming, Yunnan, 650032, China. FAU - Wang, Yulei AU - Wang Y AD - Pain Department, Qujing City Second People's Hospital, Qujing, Yunnan, 655000, China. FAU - Feng, Fanzhe AU - Feng F AD - Department of Orthopaedic Surgery, 920th Hospital of Joint Logistics Support Force, Kunming, Yunnan, 650032, China. FAU - Du, Di AU - Du D AD - Department of Orthopaedic Surgery, 920th Hospital of Joint Logistics Support Force, Kunming, Yunnan, 650032, China. FAU - Shao, Nengqi AU - Shao N AD - Department of Orthopaedic Surgery, 920th Hospital of Joint Logistics Support Force, Kunming, Yunnan, 650032, China. FAU - Zhou, Tianhua AU - Zhou T AD - Department of Orthopaedic Surgery, 920th Hospital of Joint Logistics Support Force, Kunming, Yunnan, 650032, China. FAU - Chen, Yuying AU - Chen Y AD - Xinjiang Medical University Institute of TCM, Urumqi, Xinjiang, 830017, China. FAU - Xu, Yongqing AU - Xu Y AD - Department of Orthopaedic Surgery, 920th Hospital of Joint Logistics Support Force, Kunming, Yunnan, 650032, China. FAU - Xie, Zengru AU - Xie Z AD - Department of Traumatology and Orthopedics, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830054, China. xiezenru@126.com. FAU - Cui, Yi AU - Cui Y AD - Department of Orthopaedic Surgery, 920th Hospital of Joint Logistics Support Force, Kunming, Yunnan, 650032, China. cuiyi@kmmu.edu.cn. AD - Kunming Medical University, Kunming, Yunnan, 650500, China. cuiyi@kmmu.edu.cn. LA - eng GR - ZX20191001/Funds of Yunnan Orthopedic Trauma Clinical Medical Center/ GR - 2022YFC2405704/National Key Research and Development Program/ GR - 20254916CE340013/Yunnan Province Key Laboratory of Microsurgical Medical Technology/ GR - 202505AJ310046/Yunnan Provincial Science and Technology Talent and Platform Program/ GR - 2021D01D19/Natural Science Foundation of Xinjiang Uygur Autonomous Region/ GR - 82260409/National Natural Science Foundation of China/ GR - 202405AC350079/Yunnan Province young and middle-aged academic and technical leaders reserve talent project/ GR - 2024YFC2418103/National Key Research and Development Program of China/ GR - 2024YNLCYXZX0184/Research Project of Yunnan Provincial Clinical Medical Center/ PT - Comparative Study PT - Journal Article DEP - 20260803 PL - England TA - BMC Musculoskelet Disord JT - BMC musculoskeletal disorders JID - 100968565 SB - IM MH - Humans MH - Female MH - *Lumbar Vertebrae/surgery/diagnostic imaging MH - *Pedicle Screws MH - *Spondylolysis/surgery/diagnostic imaging MH - Retrospective Studies MH - Male MH - *Spinal Fusion/instrumentation/methods MH - Middle Aged MH - Treatment Outcome MH - *Bone Plates MH - Adult MH - Aged MH - Range of Motion, Articular MH - Operative Time OTO - NOTNLM OT - Clinical outcomes OT - Internal fixation OT - Lumbar spondylolysis OT - Repair COIS- Declarations. Ethics approval and consent to participate: This study was reviewed and approved by the Institutional Review Board of the 920th Hospital of the Joint Logistics Support Force in Kunming, China (Approval Number: LS2023-152 K-01).In accordance with the Institutional Ethics Committee's ethical review process, it has been determined that the requirement for informed consent from participants is waived. This waiver of informed consent is justified by the study's retrospective, anonymous design, which involves analyzing pre-existing data without directly involving or identifying individual participants. The Institutional Ethics Committee decided to waive informed consent to facilitate the study while upholding ethical principles and protecting participants' rights and confidentiality. The waiver of informed consent was granted to streamline the research process and mitigate potential risks to participants, as their identities are not disclosed, and participation is voluntary. All procedures performed in this study involving human participants were conducted in accordance with the ethical standards of the institutional research committee and with the Declaration of Helsinki. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:51 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2024/04/19 00:00 [received] PHST- 2026/06/24 00:00 [accepted] PHST- 2026/10/30 00:51 [entrez] AID - 10.1186/s12891-026-10135-1 [pii] AID - 10.1186/s12891-026-10135-1 [doi] PST - epublish SO - BMC Musculoskelet Disord. 2026 Aug 3;27(1):930. doi: 10.1186/s12891-026-10135-1. PMID- 42958161 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-244X (Electronic) IS - 1471-244X (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Sex differences in associations between sleep architecture and CBC-derived immune cell indices in MDD with comorbid insomnia. LID - 841 [pii] LID - 10.1186/s12888-026-08470-7 [doi] AB - BACKGROUND: Insomnia symptoms and alterations in sleep architecture are core pathophysiological features of Major Depressive Disorder (MDD). While sex differences in MDD prevalence and symptomatology are well documented, the specific interplay between sleep architecture and complete blood count (CBC)-derived immune cell indices, metabolic markers, and thyroid-related parameters remains under-characterized with respect to sex. METHODS: This study included 126 patients with MDD and comorbid insomnia (43 males, 83 females). All participants underwent overnight polysomnography (PSG) and fasting blood biochemical analysis. Sex-specific associations between objective sleep parameters and peripheral biomarkers were analyzed using age-adjusted partial Spearman correlation and multiple linear regression models with interaction terms. RESULTS: Female patients had longer REM sleep latency and N2 sleep duration, whereas male patients had a higher apnea-hypopnea index. Females had higher HDL-C levels, while males had higher monocyte counts. In age-adjusted FDR-corrected correlation analyses, significant associations involving CBC-derived immune cell counts were observed primarily in male patients, where lymphocyte count was associated with TST, REM sleep duration, N1%, and N2 sleep duration; no significant CBC-derived immune cell count correlations remained in female patients. In multivariable interaction analyses with FDR correction, sex x lymphocyte interaction terms remained significant for TMA, REM sleep duration, and REM sleep duration relative to TST. CONCLUSION: These findings suggest that sex may shape both the subjective-objective expression of sleep disturbance and lymphocyte-related sleep regulation in patients with MDD and comorbid insomnia. The pattern of poorer subjective sleep quality despite relatively favorable selected PSG characteristics in females, together with sex-dependent lymphocyte associations involving REM sleep and arousal measures, warrants further validation in larger longitudinal studies. CLINICAL TRIAL NUMBER: Not applicable. CI - (c) 2026. The Author(s). FAU - Wang, Qi AU - Wang Q AD - The Affiliated Mental Health Center of Jiangnan University, Wuxi Central Rehabilitation Hospital, Wuxi, Jiangsu, 214151, China. FAU - Deng, Manli AU - Deng M AD - The Affiliated Mental Health Center of Jiangnan University, Wuxi Central Rehabilitation Hospital, Wuxi, Jiangsu, 214151, China. FAU - Wang, Yixu AU - Wang Y AD - The Affiliated Mental Health Center of Jiangnan University, Wuxi Central Rehabilitation Hospital, Wuxi, Jiangsu, 214151, China. FAU - Gu, Dongqi AU - Gu D AD - The Affiliated Mental Health Center of Jiangnan University, Wuxi Central Rehabilitation Hospital, Wuxi, Jiangsu, 214151, China. FAU - Zhu, Wei AU - Zhu W AD - The Affiliated Mental Health Center of Jiangnan University, Wuxi Central Rehabilitation Hospital, Wuxi, Jiangsu, 214151, China. FAU - Chen, Limin AU - Chen L AD - Wuxi Huishan District Qianqiao Street Community Health Service Center, Wuxi, Jiangsu, 214151, China. findition@163.com. FAU - Wang, Jun AU - Wang J AD - The Affiliated Mental Health Center of Jiangnan University, Wuxi Central Rehabilitation Hospital, Wuxi, Jiangsu, 214151, China. woodfish2@126.com. FAU - Luan, Peipei AU - Luan P AD - The Affiliated Mental Health Center of Jiangnan University, Wuxi Central Rehabilitation Hospital, Wuxi, Jiangsu, 214151, China. 9862023066@jiangnan.edu.cn. LA - eng GR - Y20242114/Wuxi Municipal Bureau on Science and Technology/ GR - K20231056/Wuxi Municipal Bureau on Science and Technology/ GR - 82300483/National Natural Science Foundation of China/ GR - Q202420/Wuxi Health and Family Planning Commission/ PT - Journal Article DEP - 20260731 PL - England TA - BMC Psychiatry JT - BMC psychiatry JID - 100968559 SB - IM MH - Humans MH - Female MH - Male MH - *Sleep Initiation and Maintenance Disorders/immunology/epidemiology/physiopathology/blood/complications MH - Polysomnography MH - Adult MH - *Major Depressive Disorder/immunology/blood/epidemiology/complications/physiopathology MH - Middle Aged MH - Sex Factors MH - *Sex Characteristics MH - Comorbidity MH - Sleep Duration MH - Blood Cell Count MH - *Sleep/physiology OTO - NOTNLM OT - CBC-derived immune cell indices OT - Inflammation OT - Major depressive disorder OT - Metabolism OT - Polysomnography OT - Sex differences OT - Sleep architecture COIS- Declarations. Ethics approval and consent to participate: All participants provided written informed consent prior to enrollment. The study protocol was approved by the Ethics Committee of Wuxi Mental Health Center (Approval No. WXMHCIRB2025LLky098). All procedures involving human participants were conducted in accordance with the ethical standards of the institutional research committee and with the 1975 Declaration of Helsinki and its later amendments. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:51 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/06 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/30 00:51 [entrez] AID - 10.1186/s12888-026-08470-7 [pii] AID - 10.1186/s12888-026-08470-7 [doi] PST - epublish SO - BMC Psychiatry. 2026 Jul 31;26(1):841. doi: 10.1186/s12888-026-08470-7. PMID- 42958158 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2458 (Electronic) IS - 1471-2458 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Network analysis of mental health literacy among adolescents and comparison of gender differences. LID - 3124 [pii] LID - 10.1186/s12889-026-28737-8 [doi] AB - BACKGROUND: Mental health literacy serves as a crucial protective factor for adolescents when coping with psychological distress. To deeply investigate the structural characteristics of adolescents' mental health literacy, this study employed network analysis to construct the network structure of adolescents' mental health literacy, identify core nodes, and compare gender differences. METHODS: A stratified random sampling method with grade as the classification variable was adopted to select 1,977 adolescents from grades 4 to 9 in Guangdong, China, to conduct the Adolescent Mental Health Literacy Scale survey. RESULTS: (1) The results of overall network estimation and inference showed that HS5 ("Talking improves mental health") in the help-seeking dimension had the highest expected influence index, followed by KMH1 ("Thoughts affect feelings") and KMI2 ("Mental disorders affect emotions"), indicating that these three nodes exerted the greatest influence on the item network. (2) The network comparison results demonstrated a significant difference between males and females in the invariance test of global strength (Male = 6.98, Female = 6.42; global strength difference = 0.56, p = 0.04). In the male network, the top three nodes in terms of expected influence index from high to low were HS3 ("Would disclose mental disorder"), HS1 ("Seek help if problems"), and HS4 ("Comfortable sharing with peers"). In the female network, the top three nodes in terms of expected influence index from high to low were HS5 ("Talking improves mental health"), KMI2 ("Mental disorders affect emotions"), and KMH1 ("Thoughts affect feelings"). CONCLUSION: The core node of adolescents' mental health literacy is HS5 ("Talking about my feelings with others helps promote mental health"). Further gender-specific analysis revealed that the core node of mental health literacy for male adolescents was HS3 ("I would actively seek help if I have psychological problems") in the help-seeking dimension, while for female adolescents, it was HS5 ("Talking about my feelings with others helps promote mental health") in the help-seeking dimension. Focusing on the help-seeking factor may be a significant intervention direction for enhancing adolescents' mental health literacy. CI - (c) 2026. The Author(s). FAU - Jiang, Zhenming AU - Jiang Z AD - Faculty of Education, Shaanxi Xueqian Normal University, No.101 Shenhe 2nd Road, Xi'an, Chang'an District, 710100, China. FAU - Li, Yan AU - Li Y AD - Faculty of Education, Shaanxi Xueqian Normal University, No.101 Shenhe 2nd Road, Xi'an, Chang'an District, 710100, China. FAU - Wei, Wei AU - Wei W AD - Faculty of Education, Shaanxi Xueqian Normal University, No.101 Shenhe 2nd Road, Xi'an, Chang'an District, 710100, China. FAU - Zhu, Jinwei AU - Zhu J AD - Faculty of Education, Shaanxi Xueqian Normal University, No.101 Shenhe 2nd Road, Xi'an, Chang'an District, 710100, China. 147618482@qq.com. LA - eng GR - S202514390100/Shaanxi Provincial College Students' Innovation and Entrepreneurship Training Program Project/ GR - 20JZ033/Key Scientific Research Program Project of Shaanxi Provincial Department of Education/ GR - 2023P010/Shaanxi Provincial Social Science Fund Project/ GR - SGH23Y2566/Shaanxi Provincial Education Science 14th Five Year Plan 2023 Project/ PT - Comparative Study PT - Journal Article DEP - 20260803 PL - England TA - BMC Public Health JT - BMC public health JID - 100968562 SB - IM MH - Humans MH - Adolescent MH - Male MH - *Health Literacy/statistics & numerical data MH - China MH - *Mental Health MH - Female MH - Sex Factors OTO - NOTNLM OT - Adolescents OT - Core nodes OT - Mental health literacy OT - Network analysis OT - Network comparison COIS- Declarations. Ethics approval and consent to participate: This study was conducted in accordance with the declaration of Helsinki and approved by the Institutional Review Board (IRB) in the Faculty of Education, Shaanxi Xueqian Normal University, China (Approval Number: 2024SNSY-09-01). Furthermore, the informed consent was obtained from all participants involved in this study. Data were collected offline, and participants were allowed to voluntarily contribute their information anonymously. We assure you that the data collected will not be shared with anyone and will be kept strictly confidential throughout this study and subsequent analysis. Consent for publication: All authors approved the final manuscript and the submission to this journal. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:51 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2024/11/17 00:00 [received] PHST- 2026/07/22 00:00 [accepted] PHST- 2026/10/30 00:51 [entrez] AID - 10.1186/s12889-026-28737-8 [pii] AID - 10.1186/s12889-026-28737-8 [doi] PST - epublish SO - BMC Public Health. 2026 Aug 3;26(1):3124. doi: 10.1186/s12889-026-28737-8. PMID- 42958156 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2458 (Electronic) IS - 1471-2458 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Toxic leadership and employee health: exploring psychosocial stress pathways in organizational settings. LID - 3127 [pii] LID - 10.1186/s12889-026-28813-z [doi] AB - Toxic leadership poses a profound threat to employee well-being, particularly within high-context, collectivist cultures where hierarchical norms often suppress resistance. Despite growing interest globally, its psychosocial effects in Middle Eastern organizational settings remain underexplored. This qualitative study examines how toxic leadership affects the health and well-being of employees working in healthcare, business, and education organizations in Jeddah, Saudi Arabia, with particular attention to lived experiences and culturally specific coping strategies. Using an interpretivist qualitative design, semi-structured interviews were conducted in English with 42 employees from healthcare (n = 10), business (n = 12), and education (n = 20) organizations in Jeddah. Participants were purposively selected based on self-reported experience of or direct observation of recurrent harmful supervisory behaviors. Data were analyzed using Braun and Clarke's six-phase thematic analysis, resulting in seven overarching themes: (1) Silent Suffering, employees experience fear, suppression, and emotional paralysis; (2) Cultural Rationalization of Abuse, submission and religious values normalize toxicity; (3) Emotional and Mental Drain, including anxiety, burnout, and psychosomatic symptoms; (4) Coping Strategies and Resistance, faith, peer support, and avoidance tactics; (5) Leadership as Untouchable Power, reinforced by favoritism and wasta; (6) Organizational Silence and HR Inaction, ineffective grievance mechanisms and distrust; and (7) Normalization and Identity Conflict, ethical erosion and emotional numbness. Findings enrich the Job Demands-Resources (JD-R) and Conservation of Resources (COR) models by contextualizing them within Saudi cultural dynamics. This study underscores the urgency for culturally aligned leadership development, mental health integration, and anti-toxic policy frameworks in the Saudi workplace. CI - (c) 2026. The Author(s). FAU - Altassan, Megren Abdullah AU - Altassan MA AUID- ORCID: 0000-0002-1595-9033 AD - Department of Human Resources Management, University of Business and Technology, Jeddah, Saudi Arabia. m.altassan@ubt.edu.sa. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - BMC Public Health JT - BMC public health JID - 100968562 SB - IM MH - *Leadership MH - Humans MH - Saudi Arabia MH - Male MH - Coping Skills MH - Adaptation, Psychological MH - Female MH - Organizational Culture MH - Qualitative Research MH - *Occupational Health MH - Adult MH - *Stress, Psychological MH - Interviews as Topic MH - Working Conditions MH - Middle Aged MH - *Occupational Stress/psychology OTO - NOTNLM OT - Employee health OT - Organizational culture OT - Power distance OT - Psychosocial stress OT - Toxic leadership COIS- Declarations. Ethics approval and consent to participate: This study was reviewed and approved by the Deanship of Scientific Research, University of Business and Technology (UBT), Jeddah, Saudi Arabia (Reference No. 103/UBT0131/IRB.26/11/2025). All procedures performed in this study involving human participants and human data were conducted in accordance with relevant ethical guidelines and regulations, including the Declaration of Helsinki. Participation was voluntary, and informed consent was obtained from all participants prior to data collection. Participants were assured that their responses would be kept confidential and that identifying information would be removed from transcripts and reported quotations. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:51 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/06/25 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/30 00:51 [entrez] AID - 10.1186/s12889-026-28813-z [pii] AID - 10.1186/s12889-026-28813-z [doi] PST - epublish SO - BMC Public Health. 2026 Aug 3;26(1):3127. doi: 10.1186/s12889-026-28813-z. PMID- 42958133 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2431 (Electronic) IS - 1471-2431 (Linking) VI - 26 IP - 1 DP - 2026 Sep 9 TI - Development and internal validation of a Septic Shock Clinical Score System (SCSS) for predicting 28-day mortality in pediatric patients: a single-center retrospective study. LID - 1079 [pii] LID - 10.1186/s12887-026-07639-z [doi] AB - BACKGROUND: Predicting short-term mortality in pediatric septic shock is difficult due to the variability of the condition and the restricted practicality of current severity scoring frameworks. This study was conducted to determine predictors of 28-day mortality in children with septic shock. The study developed the Septic Shock Clinical Score System (SCSS) and performed internal validation of the score. METHODS: This single-center retrospective observational study included pediatric patients (0-16 years) with septic shock admitted to the pediatric intensive care unit of Shanxi Children's Hospital between January 2020 and December 2024. Clinical variables, including mechanical ventilation and vasoactive medication use, and laboratory parameters obtained within the first 24 h after PICU admission were collected. Univariable logistic regression and least absolute shrinkage and selection operator (LASSO) regression were used for predictor selection. A multivariable logistic regression model was constructed, and a point-based Septic Shock Clinical Score System (SCSS) was derived. Model performance was evaluated using the area under the receiver operating characteristic curve (AUC), calibration plots with bootstrap resampling, and decision curve analysis. The primary outcome was 28-day mortality. RESULTS: A total of 123 children with septic shock were included, of whom 83 (67.5%) died within 28 days. Four variables were independently associated with mortality: mechanical ventilation (OR 23.95, 95% CI 6.97-100.54), use of >/= 2 vasoactive agents (OR 6.95, 95% CI 2.08-26.59), prolonged prothrombin time (PT > 18 s; OR 5.33, 95% CI 1.82-17.05), and elevated troponin T (TnT > 40 ng/L; OR 6.70, 95% CI 1.93-28.69). SCSS scores ranged from 0 to 5 points, with higher scores associated with an increased risk of 28-day mortality. The model demonstrated favorable apparent discrimination within the study population (AUC 0.902, 95% CI 0.839-0.965) and apparent calibration based on internal bootstrap validation with 1,000 resamples. SCSS-based risk stratification demonstrated significant differences in 28-day mortality across low-, intermediate-, and high-risk groups (23.8%, 90.0%, and 90.9%, respectively; log-rank P < 0.001). Decision curve analysis demonstrated considerable clinical utility across a wide range of threshold probabilities. CONCLUSIONS: The SCSS may represent a simple tool for early risk stratification in pediatric septic shock using variables available within the first 24 h of Pediatric Intensive Care Unit (PICU) admission. Although the model demonstrated encouraging apparent predictive performance in this single-center study population, the internal validation was performed on the final fitted model after predictor selection and may therefore provide optimistic performance estimates. Accordingly, the results should be interpreted with caution, and external validation in larger multicenter studies is required to confirm model performance and further optimize clinically meaningful risk stratification before broader clinical application. CI - (c) 2026. The Author(s). FAU - Ma, Wenxia AU - Ma W AD - Infectious department, Shanxi Children's Hospital Shanxi Maternal and Child Health Hospital, Taiyuan, 030000, China. FAU - Du, Chao AU - Du C AD - Department of Pediatric Intensive Care Unit, Shanxi Children's Hospital Shanxi Maternal and Child Health Hospital, Taiyuan, 030000, China. FAU - Li, Xue AU - Li X AUID- ORCID: 0009-0009-0539-2027 AD - Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital, Taiyuan, 030032, China. lixue@sxbqeh.com.cn. LA - eng PT - Journal Article PT - Observational Study PT - Validation Study DEP - 20260909 PL - England TA - BMC Pediatr JT - BMC pediatrics JID - 100967804 SB - IM MH - Humans MH - *Shock, Septic/mortality/diagnosis/therapy MH - Retrospective Studies MH - Infant MH - Female MH - Child, Preschool MH - Male MH - *Severity of Illness Index MH - Child MH - Adolescent MH - Intensive Care Units, Pediatric MH - Infant, Newborn MH - Logistic Models MH - China/epidemiology MH - ROC Curve MH - Prognosis MH - Hospital Mortality OTO - NOTNLM OT - Mortality prediction OT - Organ dysfunction OT - Pediatric septic shock OT - Prognostic scoring system OT - Risk stratification COIS- Declarations. Ethics approval and consent to participate: This study was approved by Ethics Committee of Shanxi Children's Hospital Shanxi Maternal and Child Health Hospital (Approval Number: IRB-WZ-2025-037). Due to the study's retrospective nature, the requirement for informed consent was waived. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:50 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/06 00:00 [received] PHST- 2026/09/01 00:00 [accepted] PHST- 2026/10/30 00:50 [entrez] AID - 10.1186/s12887-026-07639-z [pii] AID - 10.1186/s12887-026-07639-z [doi] PST - epublish SO - BMC Pediatr. 2026 Sep 9;26(1):1079. doi: 10.1186/s12887-026-07639-z. PMID- 42958132 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2466 (Electronic) IS - 1471-2466 (Linking) VI - 26 IP - 1 DP - 2026 Aug 4 TI - A non-invasive simplified scoring system to predict airway mucus plugs in asthma. LID - 512 [pii] LID - 10.1186/s12890-026-04560-0 [doi] AB - BACKGROUND: Airway mucus plugs are a core pathological feature of severe asthma and are closely associated with airflow limitation and poor prognosis. However, rapid and efficient clinical screening tools for identifying airway mucus plugs in asthma are currently lacking. OBJECTIVE: This study aimed to develop and evaluate meta‑analysis‑weighted scores for estimating the presence and severity of airway mucus plugs in adults with asthma. METHODS: A systematic review and meta-analysis of six major databases (up to February 2025) were conducted to identify significant risk factors for mucus plugs. Subsequently, a mathematical equation-based score and a simplified scoring system were constructed based on the converted standardized effect-size weights (beta). Internal validation of the scoring system was then performed using a cohort of 105 asthma patients from Tongji Hospital, Huazhong University of Science and Technology (2024 approximately 2026). RESULTS: The meta-analysis included 10 studies (837 patients) and identified age, pulmonary function parameters (FEV(1)%, FVC%, FEV(1)/FVC%), and type 2 inflammatory markers (FeNO, blood eosinophil count, IgE) as key associated factors. In the internal validation cohort, the simplified scoring system showed an AUC of 0.864 (95% CI: 0.785-0.943) for estimating mucus plug presence, with a specificity of 0.941 and sensitivity of 0.718. For estimating higher mucus plug burden, the AUC was 0.859 (95% CI: 0.777-0.941), with a sensitivity of 0.917 and specificity of 0.684. CONCLUSION: The meta-analysis-weighted scores showed promising preliminary discrimination for mucus plug presence and higher burden. These scores may assist in prioritizing patients for HRCT, though external validation is required. CI - (c) 2026. The Author(s). FAU - Wang, Zhen AU - Wang Z AD - Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. AD - National Health Commission Key Laboratory of Respiratory Diseases, Hubei Provincial Clinical Research Center for Chronic Airway Inflammatory Diseases, Wuhan, China. FAU - Xiong, Tiantian AU - Xiong T AD - Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. AD - National Health Commission Key Laboratory of Respiratory Diseases, Hubei Provincial Clinical Research Center for Chronic Airway Inflammatory Diseases, Wuhan, China. FAU - Chen, Gongqi AU - Chen G AD - Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. AD - National Health Commission Key Laboratory of Respiratory Diseases, Hubei Provincial Clinical Research Center for Chronic Airway Inflammatory Diseases, Wuhan, China. AD - Department of General Practice, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. FAU - Huang, Chunli AU - Huang C AD - Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. AD - National Health Commission Key Laboratory of Respiratory Diseases, Hubei Provincial Clinical Research Center for Chronic Airway Inflammatory Diseases, Wuhan, China. FAU - Gu, Wei AU - Gu W AD - Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. AD - National Health Commission Key Laboratory of Respiratory Diseases, Hubei Provincial Clinical Research Center for Chronic Airway Inflammatory Diseases, Wuhan, China. FAU - Jie, Huiru AU - Jie H AD - Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. AD - National Health Commission Key Laboratory of Respiratory Diseases, Hubei Provincial Clinical Research Center for Chronic Airway Inflammatory Diseases, Wuhan, China. FAU - Xia, Lixia AU - Xia L AD - Key Laboratory of Respiratory Disease of Zhejiang Province, Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, China. FAU - Chen, Ruchong AU - Chen R AD - State Key Laboratory of Respiratory Disease, National Center for Respiratory Medicine, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. FAU - Feng, Yuchen AU - Feng Y AD - Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. fengyc@tjh.tjmu.edu.cn. AD - National Health Commission Key Laboratory of Respiratory Diseases, Hubei Provincial Clinical Research Center for Chronic Airway Inflammatory Diseases, Wuhan, China. fengyc@tjh.tjmu.edu.cn. FAU - Zhen, Guohua AU - Zhen G AD - Department of Respiratory and Critical Care Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. ghzhen@tjh.tjmu.edu.cn. AD - National Health Commission Key Laboratory of Respiratory Diseases, Hubei Provincial Clinical Research Center for Chronic Airway Inflammatory Diseases, Wuhan, China. ghzhen@tjh.tjmu.edu.cn. LA - eng GR - 2024ZD0529900/Noncommunicable Chronic Diseases-National Science and Technology Major Project/ GR - 82170036/National Natural Science Foundation of China/ GR - 2024BCB042/Key Research and Development Program of Hubei Province/ GR - 2023CXZH006/Innovation and Translation Project of Tongji Hospital/ PT - Journal Article PT - Meta-Analysis PT - Systematic Review DEP - 20260804 PL - England TA - BMC Pulm Med JT - BMC pulmonary medicine JID - 100968563 SB - IM MH - Humans MH - *Asthma/physiopathology/pathology/diagnosis MH - *Mucus MH - Severity of Illness Index MH - Forced Expiratory Volume MH - Respiratory Function Tests MH - Risk Factors OTO - NOTNLM OT - Asthma OT - Clinical scoring system OT - Mucus plug OT - Risk factors OT - Severity COIS- Declarations. Ethics approval and consent to participate: The study was approved by the Ethics Committee of Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology (TJ-IRB-20210117). The patients/participants provided their written informed consent to participate in this study. Study was carried out in accordance with the Declaration of Helsinki. All methods were carried out in accordance with relevant guidelines and regulations in the Ethics approval. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:50 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/20 00:00 [received] PHST- 2026/07/29 00:00 [accepted] PHST- 2026/10/30 00:50 [entrez] AID - 10.1186/s12890-026-04560-0 [pii] AID - 10.1186/s12890-026-04560-0 [doi] PST - epublish SO - BMC Pulm Med. 2026 Aug 4;26(1):512. doi: 10.1186/s12890-026-04560-0. PMID- 42958130 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2415 (Electronic) IS - 1471-2415 (Linking) VI - 26 IP - 1 DP - 2026 Sep 15 TI - Myopia prevalence among students in nine-year and independent compulsory education schools: a retrospective cross-sectional study. LID - 697 [pii] LID - 10.1186/s12886-026-05333-3 [doi] AB - BACKGROUND: This study aims to investigate the prevalence of myopia during compulsory education in the Nanshan district of Shenzhen and the relationship between myopic prevalence and different types of educational transitions. METHODS: Three different schools were purposively selected from the Nanshan district. One independent primary school and one independent secondary school together represented the discontinuous educational transition, and one nine-year school represented the continuous educational transition. The binomial logistic regression analysis was conducted to explore educational transitions associated with myopic prevalence. RESULTS: Eight thousand ten students from two different educational transitions were involved in this retrospective cross-sectional study. The prevalence of myopia in the primary level (Grades 1-6) was 54.59%, while that in the secondary level (Grades 7-9) was 86.45%. The binomial logistic regression model revealed that the occurrence of myopia was significantly associated with females (OR = 1.60, 95% CI: 1.44-1.78; P < 0.001) and higher grade levels (P < 0.001). In exploratory analysis, students in the continuous educational transition showed lower odds of myopia than those in the discontinuous educational transition (OR = 0.85, 95% CI: 0.77-0.95; P = 0.003). CONCLUSION: The prevalence of myopia was relatively high and surged from low-grade to high-grade levels among compulsory students in the Nanshan district. An association between the educational transition model and myopia prevalence was observed. However, this association was exploratory, because the educational transition exposure is fully confounded with school identity. Further longitudinal studies with multiple schools across diverse regions are warranted to clarify the specific mechanisms through which educational structure influences ocular health. CI - (c) 2026. The Author(s). FAU - Zhou, Hui AU - Zhou H AD - Department of Ophthalmology, Southern University of Science and Technology Hospital, Shenzhen, Guangdong Province, The People's Republic of China. FAU - Xing, Baogang AU - Xing B AD - Department of Ophthalmology, Southern University of Science and Technology Hospital, Shenzhen, Guangdong Province, The People's Republic of China. FAU - Tian, Shuang AU - Tian S AD - Department of Ophthalmology, Southern University of Science and Technology Hospital, Shenzhen, Guangdong Province, The People's Republic of China. FAU - Chen, Weihua AU - Chen W AD - Department of Ophthalmology, Southern University of Science and Technology Hospital, Shenzhen, Guangdong Province, The People's Republic of China. FAU - Yang, Chengming AU - Yang C AD - Department of Ophthalmology, Southern University of Science and Technology Hospital, Shenzhen, Guangdong Province, The People's Republic of China. hanks281128@163.com. FAU - Xiao, Zixuan AU - Xiao Z AD - Department of Ophthalmology, The Chinese University of Hong Kong Shenzhen Medical Centre, Shenzhen, Guangdong Province, The People's Republic of China. zixuan_xiao@foxmail.com. LA - eng PT - Journal Article DEP - 20260915 PL - England TA - BMC Ophthalmol JT - BMC ophthalmology JID - 100967802 SB - IM MH - Humans MH - Cross-Sectional Studies MH - Retrospective Studies MH - Prevalence MH - *Myopia/epidemiology MH - Female MH - China/epidemiology MH - *Students/statistics & numerical data MH - *Schools/statistics & numerical data MH - Male MH - Child MH - Adolescent OTO - NOTNLM OT - Compulsory education OT - Educational transition OT - Myopia OT - Nanshan district OT - Retrospective Cross-sectional study COIS- Declarations. Ethics approval and consent to participate: Three schools (one independent primary school, one independent secondary school, and one nine-year school) were purposively selected from the same Sub-District in the Nanshan district of Shenzhen to represent the two different educational transition types (discontinuous educational transition vs. continuous educational transition). This geographically proximate selection was intended to minimize socioeconomic confounding across schools. This retrospective cross-sectional study analyzed data collected during the May 2025 school vision screening program. This study has been approved by the Human Medical Ethics Committee of Southern University of Science and Technology Hospital (Approval No.2026-004, dated March 2, 2026), which was in accordance with the tenets of the Declaration of Helsinki. The requirement for individual consent was waived by the committee because of the retrospective nature of the study. All methods were carried out in accordance with relevant guidelines and regulations for this study. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:50 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/10 00:00 [received] PHST- 2026/09/08 00:00 [accepted] PHST- 2026/10/30 00:50 [entrez] AID - 10.1186/s12886-026-05333-3 [pii] AID - 10.1186/s12886-026-05333-3 [doi] PST - epublish SO - BMC Ophthalmol. 2026 Sep 15;26(1):697. doi: 10.1186/s12886-026-05333-3. PMID- 42958117 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2407 (Electronic) IS - 1471-2407 (Linking) VI - 26 IP - 1 DP - 2026 Jul 30 TI - Real-world treatment pattern and clinical outcomes in Chinese NSCLC patients with KRAS G12C mutation. LID - 1327 [pii] LID - 10.1186/s12885-026-16643-6 [doi] AB - BACKGROUND: To establish a historical benchmark by describing the treatment patterns and clinical outcomes of Chinese patients with KRAS G12C-mutated advanced non-small cell lung cancer (NSCLC) in the real world, prior to the widespread availability of KRAS G12C inhibitors. METHODS: This retrospective cohort study utilized electronic medical records from four tertiary hospitals in China (January 2015 to March 2023). INCLUSION CRITERIA: age >/= 18 years, histologically confirmed locally advanced/metastatic NSCLC, KRAS G12C mutation, receipt of >/=1st line treatment, and >/= 6 months of follow-up. The primary endpoint was the proportion of different regimens used per line of therapy. Secondary endpoints included real-world overall response rate (rwORR), progression-free survival (rwPFS). Multivariable COX regression and treatment era analyses (<2020 vs. >/= 2020) were additionally conducted. RESULTS: Among 244 included patients (93.85% male, 74.57% were smokers), first-line treatment comprised chemotherapy-based (38.70%), immune-based (10.87%), chemo-immunotherapy (50.00%). The first-line rwORR was 19.1% (chemotherapy), 20% (immune-based), and 26.96% (chemo-immunotherapy), respectively. Median rwPFS was 8.25, 21.00, 16.00 months, respectively. In multivariable Cox analysis, compared to chemotherapy, chemo-immunotherapy (HR 0.39, 95% CI 0.24-0.63, p < 0.001) and immune-based therapy (HR 0.34, 95% CI 0.15-0.80, p = 0.014) showed independent rwPFS benefit. However, the immune-based group was highly enriched for PD-L1 >/= 50% (52.00% vs. 26.96% in the overall first-line cohort). STK11 mutation was a strong independent predictor of poor rwPFS (HR 5.53, 95% CI 2.77-11.05, p < 0.001). Treatment patterns shifted significantly, with first-line chemo-immunotherapy increasing from 24.64% (< 2020) to 60.87% (>/= 2020). CONCLUSIONS: This real-world study provides a comprehensive characterization of the clinical management and outcomes of KRAS G12C-mutated NSCLC in China in the pre-KRAS G12C inhibitor treatment era. First-line chemo-immunotherapy provides independent survival benefit over chemotherapy, with immune monotherapy reserved for carefully selected PD-L1 high cases. STK11 co-mutation predicts extremely poor outcomes (median PFS 3.75 months), representing an unmet clinical need. This study provides an essential historical reference for quantifying their real-world clinical impact. CI - (c) 2026. The Author(s). FAU - Shen, Lan AU - Shen L AD - Department of Medical Oncology, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 20030, China. FAU - Gao, Yibo AU - Gao Y AD - Laboratory of Translational Medicine, National Cancer Center / Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100021, China. FAU - Song, Zhengbo AU - Song Z AD - Department of Phase I Clinical Trial Ward, Zhejiang Cancer Hospital, Hangzhou, 310022, China. FAU - Wang, Qiming AU - Wang Q AD - Department of Respiratory Oncology, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, 450008, China. FAU - Zhou, Qiaoxia AU - Zhou Q AD - Department of Medical Affairs, Novartis Pharmaceuticals, Beijing, 1001102, China. FAU - Lei, Yan AU - Lei Y AD - Department of Medical Affairs, Novartis Pharmaceuticals, Beijing, 1001102, China. FAU - Lu, Shun AU - Lu S AD - Department of Medical Oncology, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 20030, China. shunlu@sjtu.edu.cn. FAU - Li, Ziming AU - Li Z AD - Department of Medical Oncology, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 20030, China. liziming1980@shsmu.edu.cn. LA - eng GR - 82473288/the National Natural Science Foundation of China/ GR - 2022XD029/Shanghai Municipal Health Commission/ GR - Y-2024AZ(EGFR)ZD-0260/Beijing Xisike Clinical Oncology Research Foundation/ GR - 2023CSJZN0600/Yangtze River Delta joint sci-tech innovation and research projects/ GR - 2023ZD0501900 & 2023ZD0501905/Noncommunicable Chronic Diseases-National Science and Technology Major Project/ GR - 2025SZ1705/Project of the Shanghai Key Laboratory of Biological Therapy for Thoracic Tumors/ GR - SHDC2024CRI066/Shanghai Hospital Development Center Project/ PT - Journal Article DEP - 20260730 PL - England TA - BMC Cancer JT - BMC cancer JID - 100967800 RN - EC 3.6.5.2 (Proto-Oncogene Proteins p21(ras)) RN - 0 (KRAS protein, human) RN - Chinese people SB - IM MH - Humans MH - *Carcinoma, Non-Small-Cell Lung/genetics/drug therapy/mortality/therapy/pathology MH - Male MH - *Lung Neoplasms/genetics/drug therapy/mortality/pathology/therapy MH - *Proto-Oncogene Proteins p21(ras)/genetics MH - Female MH - Middle Aged MH - Retrospective Studies MH - *Mutation MH - China MH - Aged MH - Treatment Outcome MH - Adult MH - East Asian People OTO - NOTNLM OT - Clinical outcome OT - KRAS G12C mutation OT - Mode of treatment OT - Non-small cell lung cancer OT - Real World Research COIS- Declarations. Ethics approval and consent to participate: This study was conducted in accordance with the Declaration of Helsinki and approved by the Ethics Committee of the Shanghai Chest Hospital, Shanghai Jiao Tong University with number of LS23058-CJDQ443B12001. All participants provided the written informed consent. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:49 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/27 00:00 [received] PHST- 2026/07/24 00:00 [accepted] PHST- 2026/10/30 00:49 [entrez] AID - 10.1186/s12885-026-16643-6 [pii] AID - 10.1186/s12885-026-16643-6 [doi] PST - epublish SO - BMC Cancer. 2026 Jul 30;26(1):1327. doi: 10.1186/s12885-026-16643-6. PMID- 42958114 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2415 (Electronic) IS - 1471-2415 (Linking) VI - 26 IP - 1 DP - 2026 Oct 27 TI - Artificial intelligence across the cataract care continuum: a systematic scoping review introducing the CAICC framework and the surgical complexity prediction gap. LID - 696 [pii] LID - 10.1186/s12886-026-05483-4 [doi] AB - BACKGROUND: Cataracts remain a leading cause of reversible blindness, and artificial intelligence (AI) applications now span the cataract care pathway. However, the distribution of these applications and their validation maturity remain unclear. This review mapped AI across the care continuum, summarized task-specific performance, characterized validation maturity, and identified evidence gaps. METHODS: The Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews (PRISMA-ScR) was followed. PubMed, Scopus, the Cochrane Library, WorldCat, and the American Academy of Ophthalmology Meeting Archive were searched; the final search was completed on 20 July 2025. Eligible studies were English-language primary research published from 1 January 2020 through 20 July 2025. A review-specific cataract artificial intelligence clinical continuum (CAICC) evidence-mapping framework was developed during data charting and synthesis. Two reviewers independently conducted study selection, data extraction, endpoint adjudication, and validation-maturity assessment. Performance was summarized descriptively; validation maturity characterized evaluation independence and clinical stage, not risk of bias or overall methodological quality. RESULTS: Of 361 reports sought for retrieval, 122 (33.8%) were not retrieved; 113/122 (92.6%) were considered plausibly eligible from available metadata. The final synthesis included 185 studies: detection (n = 52), characterization (n = 50), personalized surgical planning (n = 27), intelligent surgery (n = 28), recovery (n = 24), and patient engagement (n = 4). Among 181 validation-applicable included studies, internal testing was the most frequent highest-maturity category (123/181, 68.0%), followed by external testing (33/181, 18.2%), prospective single-center evaluation (6/181, 3.3%), and prospective multicenter evaluation (2/181, 1.1%); 17/181 (9.4%) had no independent or unclear testing. Among the retrieved and included studies, no comprehensive clinically validated multimodal preoperative cataract surgical-complexity model was identified. CONCLUSIONS: Most included studies remained at internal validation stages, and prospective multicenter evaluation was rare. Because 122/361 reports sought for retrieval were unavailable and most were considered plausibly eligible, retrieval bias may affect the comprehensiveness of the evidence map. Future research should prioritize multimodal preoperative surgical-complexity prediction, independent external testing, and prospective multicenter evaluation of locked models. REGISTRATION: Open Science Framework (OSF), DOI 10.17605/OSF.IO/ZKM86. CI - (c) 2026. The Author(s). FAU - Tjokrovonco, Ludwig Melino AU - Tjokrovonco LM AUID- ORCID: 0000-0002-3437-3855 AD - Department of Ophthalmology, Faculty of Medicine, Universitas Padjadjaran, Ir. Soekarno Street km 21, Bandung, West Java, 45363, Indonesia. ludwig17001@mail.unpad.ac.id. AD - Cicendo Hospital National Eye Center, Cicendo Street No. 4, Babakan Ciamis, Bandung, West Java, 40117, Indonesia. ludwig17001@mail.unpad.ac.id. FAU - Kartasasmita, Arief S AU - Kartasasmita AS AUID- ORCID: 0000-0003-2249-8965 AD - Department of Ophthalmology, Faculty of Medicine, Universitas Padjadjaran, Ir. Soekarno Street km 21, Bandung, West Java, 45363, Indonesia. AD - Cicendo Hospital National Eye Center, Cicendo Street No. 4, Babakan Ciamis, Bandung, West Java, 40117, Indonesia. FAU - Kartiwa, Raden Angga AU - Kartiwa RA AUID- ORCID: 0000-0001-5817-1337 AD - Department of Ophthalmology, Faculty of Medicine, Universitas Padjadjaran, Ir. Soekarno Street km 21, Bandung, West Java, 45363, Indonesia. AD - Cicendo Hospital National Eye Center, Cicendo Street No. 4, Babakan Ciamis, Bandung, West Java, 40117, Indonesia. FAU - Virgana, Rova AU - Virgana R AUID- ORCID: 0000-0002-1174-1360 AD - Department of Ophthalmology, Faculty of Medicine, Universitas Padjadjaran, Ir. Soekarno Street km 21, Bandung, West Java, 45363, Indonesia. AD - Department of Ophthalmology, Universitas Padjadjaran Hospital, Cirebon-Bandung Road, Cikeruh, Sumedang, Jatinangor, West Java, 45360, Indonesia. FAU - Prabaswara, Chandra AU - Prabaswara C AUID- ORCID: 0009-0008-8667-5063 AD - Faculty of Medicine, Universitas Sebelas Maret, Ir. Sutami Street No. 36 A, Surakarta, Central Java, 57126, Indonesia. FAU - Azuraa, Aisyah Nabila AU - Azuraa AN AUID- ORCID: 0000-0002-2660-4465 AD - Cicendo Hospital National Eye Center, Cicendo Street No. 4, Babakan Ciamis, Bandung, West Java, 40117, Indonesia. LA - eng PT - Journal Article PT - Scoping Review PT - Systematic Review DEP - 20261027 PL - England TA - BMC Ophthalmol JT - BMC ophthalmology JID - 100967802 SB - IM MH - Humans MH - *Artificial Intelligence MH - *Cataract Extraction/methods MH - *Cataract OTO - NOTNLM OT - Artificial intelligence OT - Cataract OT - Clinical decision support OT - Clinical validation OT - Intraocular lens OT - Machine learning OT - Surgical complexity COIS- Declarations. Ethics approval and consent to participate: Not applicable. This systematic scoping review synthesized previously published literature and did not involve the recruitment of human participants, collection of new human data or tissue, or animal experimentation. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:49 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/08/14 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/30 00:49 [entrez] AID - 10.1186/s12886-026-05483-4 [pii] AID - 10.1186/s12886-026-05483-4 [doi] PST - epublish SO - BMC Ophthalmol. 2026 Oct 27;26(1):696. doi: 10.1186/s12886-026-05483-4. PMID- 42958112 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2415 (Electronic) IS - 1471-2415 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Artificial intelligence and glaucoma structure-function map: a systematic review. LID - 704 [pii] LID - 10.1186/s12886-026-05175-z [doi] AB - INTRODUCTION: With advancing insights into the structure-function relationship in glaucoma, developing visual field (VF) predictive models based on optical coherence tomography (OCT)-detected structural changes have emerged as a significant research hotspot. Artificial Intelligence (AI) technologies have been widely applied in this area, demonstrating significant potential. METHODS: A comprehensive search was conducted using databases such as PubMed, Scopus, Web of Science, and Embase with key search terms including "artificial intelligence," "machine learning," "deep learning," "optical coherence tomography," "visual field," "structure-function map," and "glaucoma". Following rigorous and meticulous inclusion, exclusion, and review processes, a total of 36 studies were ultimately selected for analysis. RESULTS: Several studies reported favorable performance for predicting global VF indices, pointwise VF sensitivity, or longitudinal VF progression, although performance varied across tasks, datasets, OCT devices, VF patterns, validation designs, and performance metrics. Various visualization techniques, including heatmaps, simulation of structure-function maps, and occlusion maps, have been employed to interpret the predictions of these models. The generated structure-function maps are consistent with the current clinical understanding of the relationship between glaucoma structure and function. However, current AI models generally suffer from decreased performance in the advanced stage glaucoma and are limited by the narrow scanning range of OCT. CONCLUSIONS: Current glaucoma structure-function AI prediction models have been applied to pointwise VF prediction. However, to further deploy these models in glaucoma clinical settings, it is necessary to construct models that can predict pattern deviation probability maps based on wide-field volumetric OCT scans. CI - (c) 2026. The Author(s). FAU - Wang, Deming AU - Wang D AD - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangdong Provincial Clinical Research Center for Ocular Diseases, Sun Yat- Sen University, No. 7 Jinsui Road, Guangzhou, 510060, China. FAU - Yang, Zefeng AU - Yang Z AD - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangdong Provincial Clinical Research Center for Ocular Diseases, Sun Yat- Sen University, No. 7 Jinsui Road, Guangzhou, 510060, China. FAU - Song, Diping AU - Song D AD - Shanghai Artificial Intelligence Innovation Center, Shanghai, 200232, China. FAU - Li, Zihan AU - Li Z AD - Shanghai Artificial Intelligence Innovation Center, Shanghai, 200232, China. FAU - Zhou, Fengqi AU - Zhou F AD - Ophthalmology, Mayo Clinic Health System, Eau Claire, WI, USA. FAU - Zhang, Yinhang AU - Zhang Y AD - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangdong Provincial Clinical Research Center for Ocular Diseases, Sun Yat- Sen University, No. 7 Jinsui Road, Guangzhou, 510060, China. AD - Yunnan Eye Institute & Key Laboratory of Yunnan Province, Yunnan Eye Disease Clinical Medical Center, Affiliated Hospital of Yunnan University, Yunnan University, 176 Qingnian, Kunming, 650021, China. FAU - Jiang, Jiaxuan AU - Jiang J AD - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangdong Provincial Clinical Research Center for Ocular Diseases, Sun Yat- Sen University, No. 7 Jinsui Road, Guangzhou, 510060, China. FAU - Kong, Kangjie AU - Kong K AD - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangdong Provincial Clinical Research Center for Ocular Diseases, Sun Yat- Sen University, No. 7 Jinsui Road, Guangzhou, 510060, China. FAU - Fang, Zige AU - Fang Z AD - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangdong Provincial Clinical Research Center for Ocular Diseases, Sun Yat- Sen University, No. 7 Jinsui Road, Guangzhou, 510060, China. FAU - Liu, Xiaoyi AU - Liu X AD - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangdong Provincial Clinical Research Center for Ocular Diseases, Sun Yat- Sen University, No. 7 Jinsui Road, Guangzhou, 510060, China. FAU - Li, Fei AU - Li F AD - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangdong Provincial Clinical Research Center for Ocular Diseases, Sun Yat- Sen University, No. 7 Jinsui Road, Guangzhou, 510060, China. lifei.aletheus@gmail.com. FAU - Zhang, Xiulan AU - Zhang X AUID- ORCID: 0000-0002-9288-0767 AD - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science, Guangdong Provincial Clinical Research Center for Ocular Diseases, Sun Yat- Sen University, No. 7 Jinsui Road, Guangzhou, 510060, China. zhangxl2@mail.sysu.edu.cn. LA - eng GR - 82101117, 82571203/National Natural Science Foundation of China/ GR - 303020104/High-level Hospital Construction Project, Zhongshan Ophthalmic Center, Sun Yat-sen University/ GR - 2026P-GX005/Guangzhou Regional Clinical High-Tech Project/ GR - 2026QZSPT24/Research Funds of the State Key Laboratory of Ophthalmology/ PT - Journal Article PT - Systematic Review DEP - 20260803 PL - England TA - BMC Ophthalmol JT - BMC ophthalmology JID - 100967802 SB - IM MH - Humans MH - *Glaucoma/physiopathology/diagnosis MH - Tomography, Optical Coherence/methods MH - *Artificial Intelligence MH - *Visual Fields/physiology MH - Retinal Ganglion Cells/pathology OTO - NOTNLM OT - Artificial intelligence OT - Glaucoma OT - Optical coherence tomography OT - Structure-function map OT - Visual field COIS- Declarations. Ethics approval and consent to participate: Not applicable as it is a review. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:49 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/25 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/30 00:49 [entrez] AID - 10.1186/s12886-026-05175-z [pii] AID - 10.1186/s12886-026-05175-z [doi] PST - epublish SO - BMC Ophthalmol. 2026 Aug 3;26(1):704. doi: 10.1186/s12886-026-05175-z. PMID- 42958110 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2407 (Electronic) IS - 1471-2407 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Overexpression of small nucleolar RNA host gene family members predicts poor bladder cancer prognosis: based on a systematic review and meta-analysis. LID - 1324 [pii] LID - 10.1186/s12885-026-16508-y [doi] AB - BACKGROUND: Increasing long non-coding RNA SNHG members are aberrantly expressed in bladder cancer (BC) and substantially linked to its prognosis. This investigation aims to perform a meta-analysis to evaluate the connection between SNHG expression levels and BC prognosis. METHODS: Six relevant electronic databases were queried to identify eligible original studies per the specified inclusion and exclusion criteria. HRs and 95%CIs were obtained directly or indirectly, case numbers and totals for each clinical pathological parameter were extracted and converted into ORs and 95% CIs using Stata software. Then, HRs, ORs, and 95% CIs were aggregated to evaluate the link between SNHG expression and survival outcomes. Sensitivity analysis and publication bias detection were also executed. RESULTS: This meta-analysis ultimately incorporated 22 publications involving 1964 BC patients. Pooling HR with 95%CI showed that high SNHGs were linked to bad OS (HR = 1.496, 95%CI: 1.063-1.930). Pooling OR with 95%CI indicated the marked positive correlation between increasing SNHG expression and advanced TNM stage (OR = 1.769, 95%CI: 1.440-2.174), easier lymph node metastasis (OR = 2.476, 95%CI: 1.871-3.278), poor histological grade (OR = 1.336, 95%CI: 1.040-1.716), bigger tumor size (OR = 1.383, 95%CI: 1.118-1.711), deeper depth of invasion (OR = 4.054, 95%CI: 1.253-13.112), easier DM (OR = 2.004, 95%CI: 1.398-2.873). in the meantime, the insignificant link were also found between SNHGs expression and age (OR = 0.997, 95%CI: 0.825-1.205) and gender (OR = 0.944, 95%CI: 0.730-1.221). The sensitivity analysis of combining OS shows that the overall results remained essentially unchanged even when any single study was excluded. This suggests that the pooled HR is highly reliable and robust. Begg's test revealed no substantial publication bias in this study. CONCLUSIONS: Most SNHGs are up-regulated in BC and significantly linked to poor prognosis. SNHG may represent a potential therapeutic target and prognostic markers for BC. CI - (c) 2026. The Author(s). FAU - Hao, Wei-Ping AU - Hao WP AD - Department of Urology, Zhangzhou Affiliated Hospital of FuJian Medical University, Number of 59, Shengli West Road, Zhangzhou, Fujian Province, 363000, China. 271019479@qq.com. FAU - Lin, Ze-Bin AU - Lin ZB AD - Department of Urology, Zhangzhou Affiliated Hospital of FuJian Medical University, Number of 59, Shengli West Road, Zhangzhou, Fujian Province, 363000, China. FAU - Ke, Lin AU - Ke L AD - Department of Anesthesiology, Zhangzhou Affiliated Hospital of FuJian Medical University, Number of 59, Shengli West Road, Zhangzhou, Fujian Province, 363000, China. FAU - Liu, Hong-Jie AU - Liu HJ AD - Department of Urology, Zhangzhou Affiliated Hospital of FuJian Medical University, Number of 59, Shengli West Road, Zhangzhou, Fujian Province, 363000, China. FAU - Su, Chen-Qiang AU - Su CQ AD - Department of Urology, Zhangzhou Affiliated Hospital of FuJian Medical University, Number of 59, Shengli West Road, Zhangzhou, Fujian Province, 363000, China. FAU - Fu, Liu-Ming AU - Fu LM AD - Department of Urology, Zhangzhou Affiliated Hospital of FuJian Medical University, Number of 59, Shengli West Road, Zhangzhou, Fujian Province, 363000, China. FAU - Hong, Shao-Bo AU - Hong SB AD - Department of Urology, Zhangzhou Affiliated Hospital of FuJian Medical University, Number of 59, Shengli West Road, Zhangzhou, Fujian Province, 363000, China. LA - eng PT - Journal Article PT - Meta-Analysis PT - Systematic Review DEP - 20260803 PL - England TA - BMC Cancer JT - BMC cancer JID - 100967800 RN - 0 (RNA, Long Noncoding) RN - 0 (Biomarkers, Tumor) RN - 0 (RNA, Small Nucleolar) SB - IM MH - Humans MH - *Urinary Bladder Neoplasms/genetics/pathology/mortality MH - Prognosis MH - *RNA, Long Noncoding/genetics MH - Gene Expression Regulation, Neoplastic MH - *Biomarkers, Tumor/genetics MH - Neoplasm Staging MH - *RNA, Small Nucleolar/genetics MH - Female OTO - NOTNLM OT - Bladder cancer OT - Meta-analysis OT - SNHG OT - Survival prognosis COIS- Declarations. Ethics approval and consent to participate: No clinical patient research was performed in this study, and no ethical approval was necessary. All authors accept full responsibility for the work, ensuring that any questions about the accuracy or integrity of any component are adequately investigated and resolved. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:48 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/04/29 00:00 [received] PHST- 2026/07/06 00:00 [accepted] PHST- 2026/10/30 00:48 [entrez] AID - 10.1186/s12885-026-16508-y [pii] AID - 10.1186/s12885-026-16508-y [doi] PST - epublish SO - BMC Cancer. 2026 Aug 3;26(1):1324. doi: 10.1186/s12885-026-16508-y. PMID- 42958107 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2415 (Electronic) IS - 1471-2415 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Selective compartmental plication in small-angle convergence insufficiency exotropia: surgical outcomes and predictive model. LID - 703 [pii] LID - 10.1186/s12886-026-05164-2 [doi] AB - PURPOSE: This study aimed to evaluate a novel surgical approach combining unilateral lateral rectus recession (LRc) with compartmentalized inferior medial rectus plication (CIMRP) for treating small-angle (< 25 prism diopters, PD) convergence insufficiency intermittent exotropia (CI-IXT) in pediatric patients. METHODS: A retrospective analysis was conducted on 69 pediatric CI-IXT patients (mean age: 8.13 +/- 2.46 years; range: 4-14 years) who underwent unilateral LRc and CIMRP between September 2022 and January 2024 at Tianjin Eye Hospital. All patients completed a minimum 12-month follow-up (mean: 12.41 +/- 1.42 months; range: 12-18 months). Outcomes including near and distance deviations, near-distance difference (NDD), control scores, stereopsis, and complications were assessed preoperatively and postoperatively at 1 day, 2 months, 6 months, and final follow-up (12-18 months). Surgical success was defined as an exodeviation /= 3.5 mm for preoperative NDD > 10 PD, pending prospective validation. While this targeted technique addresses convergence insufficiency with favorable outcomes, further comparative studies are needed to define its clinical role. CI - (c) 2026. The Author(s). FAU - Ding, Juan AU - Ding J AD - Tianjin Eye Hospital, 300020, Tianjin, China. AD - Tianjin Key Lab of Ophthalmology and Visual Science, 300020, Tianjin, China. FAU - Zhu, Pin AU - Zhu P AD - Tianjin Eye Hospital, 300020, Tianjin, China. AD - Tianjin Key Lab of Ophthalmology and Visual Science, 300020, Tianjin, China. FAU - Chen, Liping AU - Chen L AD - Tianjin Eye Hospital, 300020, Tianjin, China. AD - Tianjin Key Lab of Ophthalmology and Visual Science, 300020, Tianjin, China. FAU - Hao, Rui AU - Hao R AD - Tianjin Eye Hospital, 300020, Tianjin, China. AD - Tianjin Key Lab of Ophthalmology and Visual Science, 300020, Tianjin, China. FAU - Zhang, Wei AU - Zhang W AD - Tianjin Eye Hospital, 300020, Tianjin, China. AD - Tianjin Key Lab of Ophthalmology and Visual Science, 300020, Tianjin, China. FAU - Li, Yueping AU - Li Y AD - Tianjin Eye Hospital, 300020, Tianjin, China. leeyueping@aliyun.com. AD - Tianjin Key Lab of Ophthalmology and Visual Science, 300020, Tianjin, China. leeyueping@aliyun.com. LA - eng GR - TJWJ2025MS036/Science and Technology Project of Tianjin Health Commission/ GR - NKSGY202405/Nankai University Optometry&Vision Science Institute Open Fund/ GR - NO.TJYXZDXK-016A/Tianjin Key Medical Discipline (Specialty) Construction Project/ GR - NKYKK202201/Nankai University Ophthalmology Institute Open Fund/ PT - Journal Article DEP - 20260803 PL - England TA - BMC Ophthalmol JT - BMC ophthalmology JID - 100967802 SB - IM MH - Humans MH - *Exotropia/surgery/physiopathology MH - Child, Preschool MH - Retrospective Studies MH - *Ophthalmologic Surgical Procedures/methods MH - Child MH - *Oculomotor Muscles/surgery/physiopathology MH - Adolescent MH - *Vision, Binocular/physiology MH - Female MH - Male MH - Treatment Outcome MH - Follow-Up Studies MH - Visual Acuity/physiology MH - Convergence, Ocular/physiology MH - *Ocular Motility Disorders/surgery/physiopathology OTO - NOTNLM OT - Compartmentalization. OT - Convergence insufficiency OT - Intermittent exotropia OT - Plication OT - Surgery COIS- Declarations. Ethics approval and consent to participate: This study was performed in accordance with the Declaration of Helsinki and was approved by the Ethics Committee of Tianjin Eye Hospital, China (KY202224). Written informed consent was obtained from each patient or their parents (for children < 18 years of age) prior to participation in the study. Consent for publication: We have obtained explicit written informed consent to publish all data. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:48 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/23 00:00 [received] PHST- 2026/07/23 00:00 [accepted] PHST- 2026/10/30 00:48 [entrez] AID - 10.1186/s12886-026-05164-2 [pii] AID - 10.1186/s12886-026-05164-2 [doi] PST - epublish SO - BMC Ophthalmol. 2026 Aug 3;26(1):703. doi: 10.1186/s12886-026-05164-2. PMID- 42958105 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2407 (Electronic) IS - 1471-2407 (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Comparative efficacy and toxicity of two versus three induction chemotherapy cycles in locally advanced nasopharyngeal carcinoma: a propensity score matching analysis. LID - 1322 [pii] LID - 10.1186/s12885-026-16655-2 [doi] AB - BACKGROUND: For patients with locally advanced nasopharyngeal carcinoma (LA-NPC), the optimal number of cycles of induction chemotherapy (IC) remains uncertain. This study aimed to assess the efficiency and toxicity of different cycles of IC in LA-NPC patients. METHODS: This retrospective study included 210 patients with LA-NPC and compared clinical outcomes and toxicity in two groups: 129 patients received two cycles of IC plus concurrent chemoradiotherapy (CCRT), and 81 patients received three cycles of IC plus CCRT. Propensity score matching was employed to mitigate selection bias and enhance the comparability of the two cohorts. Survival analysis was conducted using Kaplan-Meier curves, log-rank tests, and Cox proportional hazards regression analyses. RESULTS: Propensity score matching identified 81 matched pairs of patients. No significant differences were observed between the two-cycle and three-cycle groups regarding 5-year overall survival (OS) rates (88.1% vs. 80.4%, p = 0.21), 5-year locoregional relapse-free survival (LRRFS) rates (91.8% vs. 94.7%, p = 0.51), 5-year distant metastasis-free survival (DMFS) rates (80.9% vs. 84.8%, p = 0.55), or 5-year progression-free survival (PFS) rates (73.4% vs. 79.7%, p = 0.4). However, the three-cycle group had a greater incidence of grade 3/4 treatment-related acute toxicity, with the difference being significant during CCRT but not IC. Multivariate analysis identified stage IVa as an independent prognostic factor for OS, while LDH levels >/= 245 U/L were independent predictors of poorer DMFS and PFS. CONCLUSION: For patients with LA-NPC, three cycles of IC did not significantly improve patients' survival compared to two cycles of IC but rather increased the incidence of toxicity. CI - (c) 2026. The Author(s). FAU - Xie, Renxian AU - Xie R AD - Department of Radiation Oncology, Cancer Hospital of Shantou University Medical College, 7 Raoping Road, Shantou, Guangdong, 515031, P.R. China. FAU - Lin, Xiaoluan AU - Lin X AD - Department of Radiation Oncology, Cancer Hospital of Shantou University Medical College, 7 Raoping Road, Shantou, Guangdong, 515031, P.R. China. FAU - Li, Qiufeng AU - Li Q AD - Department of Radiation Oncology, Cancer Hospital of Shantou University Medical College, 7 Raoping Road, Shantou, Guangdong, 515031, P.R. China. AD - Shantou University Medical College, Shantou, P.R. China. FAU - Pan, Junqing AU - Pan J AD - Department of Radiation Oncology, Cancer Hospital of Shantou University Medical College, 7 Raoping Road, Shantou, Guangdong, 515031, P.R. China. AD - Shantou University Medical College, Shantou, P.R. China. FAU - Xie, Keyan AU - Xie K AD - Department of Radiation Oncology, Cancer Hospital of Shantou University Medical College, 7 Raoping Road, Shantou, Guangdong, 515031, P.R. China. AD - Shantou University Medical College, Shantou, P.R. China. FAU - Chen, Jianzhou AU - Chen J AD - Department of Radiation Oncology, Cancer Hospital of Shantou University Medical College, 7 Raoping Road, Shantou, Guangdong, 515031, P.R. China. FAU - Zhang, Ying AU - Zhang Y AD - Department of Radiation Oncology, Cancer Hospital of Shantou University Medical College, 7 Raoping Road, Shantou, Guangdong, 515031, P.R. China. FAU - Chen, Chuangzhen AU - Chen C AD - Department of Radiation Oncology, Cancer Hospital of Shantou University Medical College, 7 Raoping Road, Shantou, Guangdong, 515031, P.R. China. czchen2@stu.edu.cn. FAU - Zhan, Yizhou AU - Zhan Y AD - Department of Radiation Oncology, Cancer Hospital of Shantou University Medical College, 7 Raoping Road, Shantou, Guangdong, 515031, P.R. China. azhan440@163.com. LA - eng PT - Comparative Study PT - Journal Article DEP - 20260731 PL - England TA - BMC Cancer JT - BMC cancer JID - 100967800 SB - IM MH - Humans MH - Female MH - *Nasopharyngeal Carcinoma/drug therapy/pathology/mortality/therapy MH - *Induction Chemotherapy/adverse effects/methods MH - Propensity Score MH - Male MH - Retrospective Studies MH - Middle Aged MH - *Nasopharyngeal Neoplasms/pathology/drug therapy/mortality/therapy MH - Chemoradiotherapy/adverse effects MH - Adult MH - Treatment Outcome MH - *Antineoplastic Combined Chemotherapy Protocols/adverse effects/administration & dosage/therapeutic use MH - Kaplan-Meier Estimate MH - Aged MH - Neoplasm Staging OTO - NOTNLM OT - Acute toxicity OT - Concurrent chemoradiotherapy OT - Induction chemotherapy OT - Nasopharyngeal carcinoma OT - Propensity score matching OT - Treatment cycles COIS- Declarations. Ethics approval and consent to participate: This retrospective study used previously collected de-identified data. The protocol was reviewed by the Ethics Committee of the Cancer Hospital of Shantou University Medical College, and the requirement for informed consent was waived. The study was conducted in accordance with the Declaration of Helsinki. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:48 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/10/14 00:00 [received] PHST- 2026/07/29 00:00 [accepted] PHST- 2026/10/30 00:48 [entrez] AID - 10.1186/s12885-026-16655-2 [pii] AID - 10.1186/s12885-026-16655-2 [doi] PST - epublish SO - BMC Cancer. 2026 Jul 31;26(1):1322. doi: 10.1186/s12885-026-16655-2. PMID- 42958102 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2393 (Electronic) IS - 1471-2393 (Linking) VI - 26 IP - 1 DP - 2026 Aug 4 TI - Cerebral venous thrombosis during pregnancy and the puerperium in the setting of hyperhomocysteinemia and methylenetetrahydrofolate reductase c.677 C > T polymorphism. LID - 1244 [pii] LID - 10.1186/s12884-026-09748-0 [doi] AB - BACKGROUND AND OBJECTIVES: Cerebral venous thrombosis (CVT) is an uncommon but serious complication of pregnancy and the puerperium that arises in a physiologically hypercoagulable milieu. Hyperhomocysteinemia (HHcy) is a recognized metabolic risk factor for venous thrombosis, and common variants in the methylenetetrahydrofolate reductase (MTHFR) gene may influence homocysteine metabolism. However, the specific interplay between MTHFR variation, HHcy, and pregnancy-associated CVT remains insufficiently defined. This case series explored the clinical relevance of HHcy in obstetric CVT in the presence of the common MTHFR c.677 C > T polymorphism. METHODS: We conducted a retrospective single-center case series of four pregnant or postpartum patients with imaging-confirmed CVT, elevated plasma homocysteine, and documented MTHFR c.677 C > T genotypes. Clinical, radiological, laboratory, treatment, and short-term follow-up data were reviewed. RESULTS: Two patients were homozygous (TT) and two heterozygous (CT) for MTHFR c.677 C > T. All four patients had dural venous sinus thrombosis and elevated homocysteine at presentation. TT carriers showed higher homocysteine levels and appeared to have more severe neurological manifestations, including hemorrhagic venous infarction and generalized tonic-clonic seizures. CT carriers also had substantial thrombotic burden but showed comparatively less severe neurological involvement overall, although one experienced a prolonged seizure. All patients received therapeutic anticoagulation, with endovascular intervention in selected severe cases, together with folate, vitamin B(6) and B(1)(2) supplementation. Homocysteine normalized in all patients by one month, accompanied by early clinical improvement. CONCLUSIONS: In this case series, HHcy was observed in all four patients with pregnancy- or puerperium-associated CVT and the MTHFR c.677 C > T polymorphism, while higher homocysteine levels and more severe neurological manifestations were observed in the two TT carriers. These observations are exploratory and hypothesis-generating and do not support changes in routine homocysteine screening, MTHFR genotyping, or standard CVT management. Larger prospective multicenter studies are required to clarify the relationships among genotype, metabolic phenotype, and clinical outcomes. CI - (c) 2026. The Author(s). FAU - Zhang, Xiaoming AU - Zhang X AD - Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China. AD - National Clinical Research Center for Geriatric Diseases, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China. FAU - Fang, Kun AU - Fang K AD - Capital Medical University, Beijing, 100069, China. FAU - Zhou, Chen AU - Zhou C AD - Advanced Center of Stroke, Beijing Institute for Brain Disorders, Beijing, 100053, China. FAU - Ji, Xunming AU - Ji X AD - Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China. AD - National Clinical Research Center for Geriatric Diseases, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China. FAU - Meng, Ran AU - Meng R AD - Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China. victor65@126.com. AD - National Clinical Research Center for Geriatric Diseases, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China. victor65@126.com. FAU - Zhou, Da AU - Zhou D AUID- ORCID: 0000-0003-1376-4213 AD - Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China. popdoctor@foxmail.com. AD - National Clinical Research Center for Geriatric Diseases, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China. popdoctor@foxmail.com. LA - eng GR - 82171297/National Natural Science Foundation of China/ GR - 82101390/National Natural Science Foundation of China/ PT - Journal Article DEP - 20260804 PL - England TA - BMC Pregnancy Childbirth JT - BMC pregnancy and childbirth JID - 100967799 RN - EC 1.5.1.20 (Methylenetetrahydrofolate Reductase (NADPH2)) RN - EC 1.5.1.20 (MTHFR protein, human) RN - 0LVT1QZ0BA (Homocysteine) SB - IM MH - Humans MH - Female MH - *Methylenetetrahydrofolate Reductase (NADPH2)/genetics MH - Pregnancy MH - *Hyperhomocysteinemia/complications/genetics/blood MH - Adult MH - Retrospective Studies MH - *Intracranial Thrombosis/genetics MH - Postpartum Period MH - *Venous Thrombosis/genetics MH - Homocysteine/blood MH - Polymorphism, Genetic MH - *Puerperal Disorders/genetics OTO - NOTNLM OT - Cerebral venous thrombosis OT - Hyperhomocysteinemia OT - MTHFR polymorphism OT - Pregnancy OT - Thrombophilia COIS- Declarations. Ethics approval and consent to participate: This retrospective case series was approved by the Institutional Review Board of Xuanwu Hospital, Capital Medical University [2022; 004]. Written informed consent was obtained from all individual participants included in the study. All procedures conformed to the Declaration of Helsinki and reporting followed the CARE guidance adapted for case series. Consent for publication: Written informed consent for publication of clinical details and imaging was obtained from all participants. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:48 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/01/09 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/30 00:48 [entrez] AID - 10.1186/s12884-026-09748-0 [pii] AID - 10.1186/s12884-026-09748-0 [doi] PST - epublish SO - BMC Pregnancy Childbirth. 2026 Aug 4;26(1):1244. doi: 10.1186/s12884-026-09748-0. PMID- 42958101 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-230X (Electronic) IS - 1471-230X (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Methylation-sensitive restriction endonuclease-based amplification assay for detecting DNA methylation in colorectal cancer: a cross-sectional study. LID - 713 [pii] LID - 10.1186/s12876-026-05034-7 [doi] AB - BACKGROUND: This study evaluates the clinical utility of combined SDC2 and ADHFE1 methylation analysis for colorectal cancer (CRC) detection using a methylation-sensitive restriction endonuclease (MSRE)-based assay. METHODS: Leveraging the specificity of MSRE, we developed a streamlined amplification assay that directly analyzes methylation status of SDC2 and ADHFE1 after mixing nucleic acids with PCR reagent. A cohort of 274 participants was stratified into six groups: CRC (n = 117), advanced adenoma (AA, n = 22), polyps (n = 30), intestinal disease control (IDC, n = 39), other gastrointestinal disease control (OGDC, n = 24), and healthy controls (HC, n = 42). SDC2 and ADHFE1 methylation levels in fecal samples were assessed, and diagnostic performance was evaluated using receiver operating characteristic (ROC) curve analysis. RESULTS: The CRC group exhibited significantly higher SDC2 and ADHFE1 methylation positivity rates compared to all comparator groups, including AA, polyps, IDC, OGDC, and HC (P < 0.01). No significant differences were observed in the methylation positivity rates of SDC2, ADHFE1 or their combination across different TNM stages and levels of tumor differentiation. Joint SDC2 and ADHFE1 analysis increased sensitivity for CRC detection to 82.9% (vs. 64.1% and 65.0% for ADHFE1 and SDC2) with an AUC of 0.865 (95% CI: 0.817-0.913). For early-stage CRC (stages I + II), the dual-gene panel achieved an AUC of 0.872. However, the ability to discriminate between AA and HC (AUC: 0.693) or IDC (AUC: 0.615) remained limited in our dual-gene assay. CONCLUSIONS: The combined SDC2/ADHFE1 MSRE-based assay significantly improves diagnostic sensitivity for CRC, demonstrating superior differentiation of CRC from benign intestinal pathologies and precancerous lesions (AA and polyps). This non-invasive approach shows promise as a supplementary tool for CRC screening. CI - (c) 2026. The Author(s). FAU - Wu, Jiali AU - Wu J AD - Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan road II, Guangzhou, Guangdong, China. FAU - Li, Zhikang AU - Li Z AD - Laboratory of General Surgery, The First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan road II, Guangzhou, Guangdong, China. FAU - Ai, Lu AU - Ai L AD - Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan road II, Guangzhou, Guangdong, China. FAU - Huang, Anzhi AU - Huang A AD - Guangdong Cardiovascular Institute, Medical Research Institute, School of Basic Medical Science, Guangdong Provincial People's Hospital, Guangzhou, Guangdong, China. FAU - Huang, Shayuanzi AU - Huang S AD - Department of Laboratory Medicine, Guangxi Hospital Division of The First Affiliated Hospital of Sun Yat-sen University, Nanning, Guangxi, China. FAU - Zhang, Ruizhao AU - Zhang R AD - Guangzhou Quality Testing & Inspection Institute, Guangzhou, Guangdong, China. FAU - He, Guoshan AU - He G AD - Guangzhou Quality Testing & Inspection Institute, Guangzhou, Guangdong, China. FAU - He, Meifang AU - He M AD - Laboratory of General Surgery, The First Affiliated Hospital of Sun Yat-Sen University, 58 Zhongshan road II, Guangzhou, Guangdong, China. hemeifang@mail.sysu.edu.cn. FAU - Wang, Ruizhi AU - Wang R AD - Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan road II, Guangzhou, Guangdong, China. wangrzh3@mail.sysu.edu.cn. AD - Advanced Medical Technology Center, The First Affiliated Hospital, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong, China. wangrzh3@mail.sysu.edu.cn. FAU - Wang, Dong AU - Wang D AD - Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, 58 Zhongshan road II, Guangzhou, Guangdong, China. wdong@mail.sysu.edu.cn. AD - Department of Laboratory Medicine, Guangxi Hospital Division of The First Affiliated Hospital of Sun Yat-sen University, Nanning, Guangxi, China. wdong@mail.sysu.edu.cn. AD - Guangdong Provincial Clinical Research Center for Laboratory Medicine, Guangzhou, P. R. China. wdong@mail.sysu.edu.cn. LA - eng GR - 2025B04J0013/Guangzhou Science and technology plan project/ GR - 2023B04J0407/Guangzhou Science and technology plan project/ GR - 2022A1515220130)/GuangDong Basic and Applied Basic Research Foundation/ GR - 2024A1515011089/Guangdong Natural Science Foundation/ GR - 81972750/National Natural Science Foundation of China/ GR - 2023B110008/Guangdong Provincial Clinical Research Center for Laboratory Medicine/ GR - NO. S2025030/the Guangxi Medical and Health Appropriate Technology Development, Promotion and Application Project/ PT - Journal Article DEP - 20260803 PL - England TA - BMC Gastroenterol JT - BMC gastroenterology JID - 100968547 RN - 149769-25-5 (Syndecan-2) RN - 0 (SDC2 protein, human) RN - EC 3.1.21.- (DNA Restriction Enzymes) RN - EC 1.1.99.24 (hydroxyacid-oxoacid transhydrogenase) RN - 0 (Biomarkers, Tumor) RN - EC 1.1.- (Alcohol Oxidoreductases) RN - 0 (Mitochondrial Proteins) SB - IM MH - Humans MH - *Colorectal Neoplasms/genetics/diagnosis MH - *DNA Methylation MH - *Syndecan-2/genetics MH - Female MH - Middle Aged MH - Male MH - Cross-Sectional Studies MH - Aged MH - Adenoma/genetics/diagnosis MH - ROC Curve MH - Sensitivity and Specificity MH - DNA Restriction Enzymes MH - Feces/chemistry MH - Case-Control Studies MH - Biomarkers, Tumor/genetics MH - Alcohol Oxidoreductases MH - Mitochondrial Proteins OTO - NOTNLM OT - ADHFE1 OT - Colorectal cancer OT - Methylation OT - Methylation-sensitive restriction endonuclease OT - SDC2 COIS- Declarations. Ethics approval and consent to participate: Our study was approved by the institutional review board of the First Affiliated Hospital of Sun Yat- sen University. All patients received detailed pretest counseling and informed written consent was obtained from each participant. Our study adhered to the Declaration of Helsinki. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:48 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/07/10 00:00 [received] PHST- 2026/06/16 00:00 [accepted] PHST- 2026/10/30 00:48 [entrez] AID - 10.1186/s12876-026-05034-7 [pii] AID - 10.1186/s12876-026-05034-7 [doi] PST - epublish SO - BMC Gastroenterol. 2026 Aug 3;26(1):713. doi: 10.1186/s12876-026-05034-7. PMID- 42958100 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2407 (Electronic) IS - 1471-2407 (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - A novel predictive model for DNA mismatch repair deficient colorectal carcinoma for cost-effective MMR testing in low-resource settings: HERALD-MMR (Histological Evaluation and Risk Assessment using Local Demographics for Mismatch Repair). LID - 1321 [pii] LID - 10.1186/s12885-026-16615-w [doi] AB - BACKGROUND: Identifying dMMR (mismatch repair deficiency)/MSI (microsatellite instability) status in colorectal cancer (CRC) is standard in developed countries and is valuable for prognosis, management, and family screening. Universal testing is unfeasible in developing nations due to economic and resource constraints, limiting essential testing for optimal patient care. This study investigated the prevalence of dMMR/MSI CRC in Sri Lanka and introduced a predictive score for CRC patients in our population, which can be used to guide the selection of patients for cost-effective dMMR/MSI testing. METHODS: A cross-sectional analysis was conducted on colorectal carcinomas from Teaching Hospital Peradeniya, National Hospital Kandy, Colombo South Teaching Hospital, and General Sir John Kotelawala Defence University Hospital for two and a half years. Data collected included gender, age, tumour site, TNM stage, and family history of CRC. Haematoxylin and eosin-stained tumour slides of colectomy specimens were assessed for histopathological features. Immunohistochemistry (IHC) was performed for the four DNA mismatch repair proteins MLH1, MSH2, PMS2, and MSH6. Cases that were inconclusive by IHC were tested for MSI using PCR. Descriptive analysis included frequency, percentage, mean, standard deviation (SD) and range. Multivariable binomial logistic regression was conducted to select predictive variables. RESULTS: Of 185 patients, 104 (56.2%) were women. The mean age was 62.5 years (SD:11.8; range: 19-84). Of all cases, 23 (12.4%) were found to be mismatch repair deficient. Multivariable analysis showed significant association with age less than 60 years (p = 0.001), right sided tumour (p = 0.001), family history of CRC (at least one first or second degree relative) (p = 0.028), mucin percentage >/= 20% (p = 0.038), solid region percentage >/= 15% (p = 0.019), highest iTILs >/= 3 per high power field (p < 0.001), and Crohn-like peritumoral inflammation (p = 0.004). Considering the intercept values, rounded off to integers, a score was obtained for predicting dMMR/MSI CRC. The cutoff value for the prediction of dMMR status was taken as >/= 6 from a maximum value of 20 (sensitivity: 0.957, specificity: 0.681). CONCLUSIONS: In resource-limited settings, screening CRC patients with the proposed predictive score could cost-effectively guide dMMR/MSI testing. This preliminary score needs further validation in a larger independent cohort to ensure its reliability and broader applicability. CI - (c) 2026. The Author(s). FAU - Kosgallana, Warunie AU - Kosgallana W AUID- ORCID: 0000-0002-1350-4844 AD - Faculty of Graduate Studies, University of Sri Jayewardenepura, Nugegoda, Sri Lanka. AD - Department of Anatomy, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka. FAU - Wijetunge, Sulochana AU - Wijetunge S AUID- ORCID: 0000-0002-0587-9523 AD - Department of Pathology, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka. FAU - Malaviarachchi, Sachini AU - Malaviarachchi S AUID- ORCID: 0000-0001-6799-9080 AD - Department of Oncology, University Hospitals Plymouth NHS Trust, Plymouth, UK. FAU - Prathapan, Shamini AU - Prathapan S AUID- ORCID: 0000-0002-6503-0396 AD - Department of Community Medicine, Faculty of Medical Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka. FAU - Hegoda, Sanwari AU - Hegoda S AUID- ORCID: 0009-0008-7281-0914 AD - Department of Pathology, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka. FAU - Ratnatunga, Kesara AU - Ratnatunga K AUID- ORCID: 0000-0002-4000-4108 AD - Department of Surgery, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka. FAU - Ratnayake, Palitha AU - Ratnayake P AUID- ORCID: 0000-0002-6322-9617 AD - National Hospital Kandy, Kandy, Sri Lanka. FAU - Kandegedara, Senaka AU - Kandegedara S AD - National Hospital Kandy, Kandy, Sri Lanka. FAU - Gamage, Bawantha AU - Gamage B AUID- ORCID: 0000-0003-4381-228X AD - Department of Surgery, Faculty of Medical Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka. FAU - Kumarasinghe, Iranthi AU - Kumarasinghe I AUID- ORCID: 0000-0001-8530-4545 AD - Department of Paraclinical Sciences, Faculty of Medicine, General Sir John Kotelawala Defence University, Ratmalana, Sri Lanka. FAU - Taylor, Siobhan AU - Taylor S AUID- ORCID: 0009-0001-5286-0008 AD - Gloucestershire Hospitals NHS Foundation Trust, Gloucestershire, UK. FAU - Prematilleke, Isha AU - Prematilleke I AUID- ORCID: 0000-0001-5323-225X AD - Department of Pathology/Centre for Cancer Research, Faculty of Medical Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka. ishaprem@sjp.ac.lk. LA - eng GR - URG/2022/55/M, URG/2024-CG-2022/55-29/M/University of Peradeniya/ GR - URG/2021/26/M/University of Peradeniya/ GR - ASP/01/RE/MED/2022/49/University of Sri Jayewardenepura/ PT - Journal Article DEP - 20260731 PL - England TA - BMC Cancer JT - BMC cancer JID - 100967800 RN - EC 3.6.1.3 (MutS Homolog 2 Protein) RN - EC 3.6.1.3 (MSH2 protein, human) RN - 0 (DNA-Binding Proteins) RN - EC 3.6.1.3 (MutL Protein Homolog 1) RN - EC 3.6.1.3 (Mismatch Repair Endonuclease PMS2) RN - 0 (MLH1 protein, human) SB - IM MH - Humans MH - *Colorectal Neoplasms/genetics/pathology/diagnosis/epidemiology MH - *DNA Mismatch Repair/genetics MH - Female MH - Microsatellite Instability MH - Middle Aged MH - Male MH - Cross-Sectional Studies MH - Cost-Benefit Analysis MH - Risk Assessment MH - MutS Homolog 2 Protein/genetics MH - Aged MH - Sri Lanka/epidemiology MH - Adult MH - Resource-Limited Settings MH - DNA-Binding Proteins/genetics MH - MutL Protein Homolog 1/genetics MH - Mismatch Repair Endonuclease PMS2/genetics MH - Immunohistochemistry MH - Neoplastic Syndromes, Hereditary/diagnosis/genetics OTO - NOTNLM OT - Colorectal carcinoma OT - DNA mismatch repair deficiency OT - Microsatellite instability OT - Predictive score COIS- Declarations. Ethics approval and consent to participate: The study adhered to the Helsinki Declaration (1975, revised 2008). Informed consent was obtained from all participants included in the study. Ethical clearance was granted for this study by the Research Ethics Committee, Faculty of Medical Sciences, University of Sri Jayewardenepura (Ref: 12/22) with administrative clearance from all involved institutions and the Ministry of Health, Sri Lanka (ETR/AC/M3/16/2022). Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:48 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/27 00:00 [received] PHST- 2026/07/21 00:00 [accepted] PHST- 2026/10/30 00:48 [entrez] AID - 10.1186/s12885-026-16615-w [pii] AID - 10.1186/s12885-026-16615-w [doi] PST - epublish SO - BMC Cancer. 2026 Jul 31;26(1):1321. doi: 10.1186/s12885-026-16615-w. PMID- 42958099 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2393 (Electronic) IS - 1471-2393 (Linking) VI - 26 IP - 1 DP - 2026 Aug 4 TI - Maternal loneliness and associated factors among postnatal mothers in Boneya Boshe Woreda, Western Ethiopia, 2024: community-based cross-sectional study design. LID - 1245 [pii] LID - 10.1186/s12884-026-09412-7 [doi] AB - INTRODUCTION: A person's subjective perception of the discrepancy between their expected and actual social engagement levels is known as loneliness. Less research has been conducted on it in low-income countries, although it affects the health of both mothers and infants. OBJECTIVE: The study aimed to assess the prevalence of maternal loneliness and associated factors among postnatal mothers in Boneya Boshe Woreda, Western Ethiopia. METHODS AND STUDY PERIOD: A community-based cross-sectional study was conducted from January 1 to January 30, 2024. A simple random sampling method and a face-to-face interviewer-administered structured questionnaire were used to collect the data. The data were analyzed using SPSS software version 26. Using the logistic regression model, bivariate and multivariable analyses were done to identify associated factors. The level of statistical significance was declared at 95% CI with a p-value < 0.05. RESULTS: From a total of 614 selected postnatal mothers, 605 mothers participated in the study, with a response rate of 98.53%. The findings showed that the prevalence of maternal loneliness after childbirth was 36.86% (95% CI: 34.23, 38.12). Not having post-natal follow-up (AOR = 2.90; 95% CI: 1.24, 3.66), unplanned pregnancy (AOR = 2.97; 95% CI: 2.92, 4.45), husbands involvement in maternity and child health care (AOR = 3.93; 95% CI: 3.82, 5.60), intimate partner violence (AOR = 2.04; 95% CI: 1.64, 5.65), and husbands alcohol consumption (AOR = 4.12; 95% CI: 4.15, 7.10) were factors that significantly influence maternal loneliness. CONCLUSIONS: As it was identified by this study, the prevalence of maternal loneliness was a significant health problem. Therefore, based on the identified factors, the concerned body should pay attention to them to overcome the problem. CI - (c) 2026. The Author(s). FAU - Wedajo, Lema Fikadu AU - Wedajo LF AD - Department of Midwifery, Institute of Health Sciences, Wallaga University, Nekemte, Ethiopia. lemafika2014@gmail.com. FAU - Kitil, Gemeda Wakgari AU - Kitil GW AD - Department of Midwifery, College of Health Science, Mattu University, Mattu, Ethiopia. FAU - Teferi, Sheleme Mengistu AU - Teferi SM AD - Department of Midwifery, College of Medicine and Health Sciences, Madda Walabu University, Robe, Ethiopia. FAU - Bekele, Firomsa AU - Bekele F AD - Department of Pharmacy, Institute of Health Sciences, Wallaga University, Nekemte, Ethiopia. FAU - Kabsha, Shula Shusho AU - Kabsha SS AD - Department of Midwifery, College of Medicine and Health Sciences, Arba Minch University, Arba Minch, Ethiopia. FAU - Degefa, Bekam Dibaba AU - Degefa BD AD - Department of Midwifery, College of Health Science, Mattu University, Mattu, Ethiopia. FAU - Wedajo, Bilisuma Fikadu AU - Wedajo BF AD - Department Public Health Officer, School of Public Health, Wallaga University, Nekemte, Ethiopia. FAU - Hajure, Mohammedamin AU - Hajure M AD - Department of Psychiatry, College of Medicine and Health Science, Madda Walabu University, Robe, Ethiopia. FAU - Hailu, Alemu Merga AU - Hailu AM AD - Department of Midwifery, Institute of Health Sciences, Wallaga University, Nekemte, Ethiopia. FAU - Gezimu, Wubishet AU - Gezimu W AD - Department of Nursing, College of Health Science, Mattu University, Mattu, Ethiopia. FAU - Demsash, Addisalem Workie AU - Demsash AW AD - Department of Health Informatics, Asrat Woldeyes Health Science Campus, Debre Berhan University, Debre Berhan, Ethiopia. FAU - Dube, Geleta Nenko AU - Dube GN AD - Department of Health Informatics, College of Health Science, Mattu University, Mattu, Ethiopia. FAU - Alemu, Solomon Seyife AU - Alemu SS AD - Department of Midwifery, College of Medicine and Health Sciences, Madda Walabu University, Robe, Ethiopia. LA - eng PT - Journal Article DEP - 20260804 PL - England TA - BMC Pregnancy Childbirth JT - BMC pregnancy and childbirth JID - 100967799 SB - IM MH - Humans MH - Female MH - Ethiopia/epidemiology MH - Cross-Sectional Studies MH - *Loneliness/psychology MH - *Mothers/psychology/statistics & numerical data MH - Adult MH - Prevalence MH - Young Adult MH - Surveys and Questionnaires MH - *Postpartum Period/psychology MH - Risk Factors MH - Pregnancy MH - Logistic Models OTO - NOTNLM OT - Boneya Boshe OT - Cross-sectional study OT - Loneliness OT - Postnatal mothers COIS- Declarations. Ethics approval and consent to participate: An ethical clearance letter was obtained from the Wallaga University Research Ethics Review Committee with reference number WU/RD/735. All the methods were performed following the principles of the Helsinki Declaration. A letter of permission was obtained from the Boneya Boshe Woreda Health Department. Voluntary written informed consent and signatures were obtained before starting the interview from the study participants Consent for publication: It is not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:48 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2024/08/08 00:00 [received] PHST- 2026/06/01 00:00 [accepted] PHST- 2026/10/30 00:48 [entrez] AID - 10.1186/s12884-026-09412-7 [pii] AID - 10.1186/s12884-026-09412-7 [doi] PST - epublish SO - BMC Pregnancy Childbirth. 2026 Aug 4;26(1):1245. doi: 10.1186/s12884-026-09412-7. PMID- 42958095 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-230X (Electronic) IS - 1471-230X (Linking) VI - 26 IP - 1 DP - 2026 Sep 15 TI - Quality and content assessment of short videos as sources of health information about gastroscopy in China: a cross-sectional study. LID - 710 [pii] LID - 10.1186/s12876-026-05342-y [doi] AB - BACKGROUND: Gastroscopy is pivotal in diagnosing and treating gastrointestinal disorders. The rise of short-video platforms has facilitated public access to information about gastroscopy; however, the overall quality of these videos remains uncertain. This study aimed to evaluate the quality and content of gastroscopy-related videos available on social media. METHODS: Searches were conducted on Douyin, Bilibili, and Kuaishou using the Chinese term for "gastroscopy." After screening, 274 videos were selected for analysis. Quantitative content analysis was performed using the modified DISCERN, GQS, and JAMA scoring systems. RESULTS: Medical professionals uploaded 59.9% of the videos, with Douyin having the highest proportion. Videos by medical professionals demonstrated higher reliability than those from non-medical sources. Moreover, videos on Bilibili had higher modified DISCERN and GQS scores, whereas videos on Douyin had the highest JAMA scores. Nonetheless, overall completeness and reliability were limited when evaluated against a comprehensive educational standard, as many videos omitted essential information on contraindications and postoperative precautions. Non-medical videos focused primarily on preoperative preparation and examination process, providing limited information on definition, contraindications, and postoperative precautions. In contrast, videos produced by medical professionals demonstrated consistency across the seven evaluated aspects, with a particular emphasis on the indications for gastroscopy. Correlation analysis indicated that the modified DISCERN score was negatively associated with likes, shares, collections, and comments; however, these associations should be interpreted cautiously, as engagement metrics may reflect factors beyond content quality. Conversely, the GQS score showed no significant correlation with these engagement metrics, while the JAMA score was only negatively correlated with comments. CONCLUSION: The completeness and reliability of information presented in gastroscopy‑related videos are generally limited when assessed as comprehensive patient‑education resources, and vary considerably across sources. Important topics, particularly contraindications and postoperative precautions, are frequently underrepresented. These findings highlight the need to improve the completeness and reliability of gastroscopy-related information on short-video platforms. Future research should explore strategies to optimize the quality and delivery of online medical content. CI - (c) 2026. The Author(s). FAU - Zhang, Dan AU - Zhang D AD - Department of Gastroenterology, Dali Bai Autonomous Prefecture People's Hospital, The Third Affiliated Hospital of Dali University, Dali, China. FAU - Li, Lanyi AU - Li L AD - Department of Gastroenterology, Dali Bai Autonomous Prefecture People's Hospital, The Third Affiliated Hospital of Dali University, Dali, China. FAU - Yang, Rongna AU - Yang R AD - Department of Gastroenterology, Dali Bai Autonomous Prefecture People's Hospital, The Third Affiliated Hospital of Dali University, Dali, China. FAU - Zhu, Ziyun AU - Zhu Z AD - Department of Gastroenterology, Dali Bai Autonomous Prefecture People's Hospital, The Third Affiliated Hospital of Dali University, Dali, China. FAU - Wang, Xuemin AU - Wang X AD - Department of Gastroenterology, Dali Bai Autonomous Prefecture People's Hospital, The Third Affiliated Hospital of Dali University, Dali, China. FAU - Zhang, Ni AU - Zhang N AD - Department of Nursing, Dali University, Dali, China. FAU - Ma, Juanjuan AU - Ma J AD - Department of Gastroenterology, Dali Bai Autonomous Prefecture People's Hospital, The Third Affiliated Hospital of Dali University, Dali, China. FAU - Duan, De AU - Duan D AD - Department of Gastroenterology, Dali Bai Autonomous Prefecture People's Hospital, The Third Affiliated Hospital of Dali University, Dali, China. FAU - Geng, Zuotao AU - Geng Z AD - Department of Urology, Dali Bai Autonomous Prefecture People's Hospital, The Third Affiliated Hospital of Dali University, Dali, China. 19028720901@163.com. FAU - Xiong, Tianqin AU - Xiong T AD - Department of Gastroenterology, Dali Bai Autonomous Prefecture People's Hospital, The Third Affiliated Hospital of Dali University, Dali, China. xtq19680308@163.com. LA - eng GR - 2025J0786/the Scientific Research Fund Project of Yunnan Education Department/ GR - 202501AC070911/Yunnan Provincial Department of Science and Technology-Kunming Medical University Joint Special Fund on Basic Research/ PT - Journal Article DEP - 20260915 PL - England TA - BMC Gastroenterol JT - BMC gastroenterology JID - 100968547 SB - IM MH - Humans MH - China MH - Cross-Sectional Studies MH - *Video Recording/standards MH - *Gastroscopy MH - *Social Media/standards MH - Reproducibility of Results MH - *Consumer Health Information/standards OTO - NOTNLM OT - Gastroscopy OT - Gastroscopy-related videos OT - Patient education OT - Quality OT - Reliability OT - Social media COIS- Declarations. Ethics approval and consent to participate: This study involved a retrospective, non-interventional content analysis of publicly available data from Douyin, Bilibili, and Kuaishou. In accordance with local legislation and institutional requirements, the study was exempt from ethical review and approval, and written informed consent was not required. All social media data were accessed and analyzed in compliance with the respective platforms' terms of use and all applicable regulations. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:48 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/09 00:00 [received] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/30 00:48 [entrez] AID - 10.1186/s12876-026-05342-y [pii] AID - 10.1186/s12876-026-05342-y [doi] PST - epublish SO - BMC Gastroenterol. 2026 Sep 15;26(1):710. doi: 10.1186/s12876-026-05342-y. PMID- 42958093 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2342 (Electronic) IS - 1471-2342 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Development and validation of a preoperative biparametric MRI-based habitat model for risk stratification of non-muscle-invasive bladder cancer. LID - 563 [pii] LID - 10.1186/s12880-026-02630-7 [doi] AB - BACKGROUND: Accurate preoperative risk stratification of non-muscle-invasive bladder cancer (NMIBC) is essential for developing individualized treatment plans. This study developed an interpretable habitat model based on biparametric MRI to improve preoperative risk stratification in patients with NMIBC. METHODS: This two-center study enrolled a total of 206 patients. The internal dataset (n = 147) included the training set (n = 81), test set (n = 35), and time-sequential validation set (n = 31). The external dataset included 59 patients. The primary outcome was the dichotomized risk stratification according to the European Association of Urology (EAU) guidelines (low-intermediate risk vs. high-very high risk). K-means clustering was applied to segment tumors into habitat subregions. Following habitat feature extraction and selection, six habitat models were constructed, including support vector machine (SVM), logistic regression, linear discriminant analysis (LDA), Gaussian process classifier, random forest, and multilayer perceptron (MLP). For comparison, a VI-RADS model and a clinical prediction model were also developed. SHapley Additive exPlanations (SHAP) analysis was used to interpret the optimal model and visualize its decision-making process. Furthermore, we conducted propensity score matching (PSM) analysis to evaluate the generalizability of the habitat models. RESULTS: All habitat models exhibited good diagnostic performance. The SVM habitat model achieved the highest AUC in both the training set (AUC = 0.95, 95% CI: 0.85-1.00) and the external validation set (AUC = 0.82, 95% CI: 0.61-1.00). It showed a trend toward better performance compared to the clinical model (AUC = 0.68, 95% CI: 0.51-0.83) and the VI-RADS model (AUC = 0.75, 95% CI: 0.58-0.92) in both sets, although the differences did not reach statistical significance. After PSM balancing all baseline covariates (all SMD < 0.1), SVM still maintained stable predictive capacity (AUC = 0.72, sensitivity = 0.769) in matched external cohort, verifying good cross-center generalization. CONCLUSION: Our biparametric MRI-based habitat models, particularly the SVM classifier, showed a trend toward improved preoperative risk stratification of NMIBC. These findings require large multicenter prospective validation before clinical individualized treatment decisions and follow-up planning. CI - (c) 2026. The Author(s). FAU - He, Pingping AU - He P AD - Department of Radiology, The First Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Taijiang District, Fuzhou, Fujian, 350005, China. FAU - Chen, Ruiquan AU - Chen R AD - Department of Radiology, The First Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Taijiang District, Fuzhou, Fujian, 350005, China. AD - Department of Radiology, Binhai Campus of the First Affiliated Hospital, National Regional Medical Center, Fujian Medical University, Fuzhou, 350212, China. FAU - Zheng, Qiaozhen AU - Zheng Q AD - Department of Radiology, The First Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Taijiang District, Fuzhou, Fujian, 350005, China. FAU - Tian, Minxuan AU - Tian M AD - Department of Radiology, The First Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Taijiang District, Fuzhou, Fujian, 350005, China. FAU - Chen, Ming AU - Chen M AD - Department of Radiology, The First Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Taijiang District, Fuzhou, Fujian, 350005, China. AD - Department of Radiology, Binhai Campus of the First Affiliated Hospital, National Regional Medical Center, Fujian Medical University, Fuzhou, 350212, China. FAU - Guo, Wei AU - Guo W AD - Department of Radiology, The First Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Taijiang District, Fuzhou, Fujian, 350005, China. guowei_fy@fjmu.edu.cn. AD - Department of Radiology, Binhai Campus of the First Affiliated Hospital, National Regional Medical Center, Fujian Medical University, Fuzhou, 350212, China. guowei_fy@fjmu.edu.cn. FAU - You, Ruixiong AU - You R AD - Department of Radiology, The First Affiliated Hospital of Fujian Medical University, 20 Chazhong Road, Taijiang District, Fuzhou, Fujian, 350005, China. yrx0507@126.com. AD - Department of Radiology, Binhai Campus of the First Affiliated Hospital, National Regional Medical Center, Fujian Medical University, Fuzhou, 350212, China. yrx0507@126.com. LA - eng PT - Journal Article PT - Multicenter Study PT - Validation Study DEP - 20260803 PL - England TA - BMC Med Imaging JT - BMC medical imaging JID - 100968553 SB - IM MH - Humans MH - *Non-Muscle Invasive Bladder Neoplasms/diagnostic imaging MH - *Magnetic Resonance Imaging/methods MH - Risk Assessment/methods MH - Female MH - Male MH - Aged MH - Middle Aged MH - Support Vector Machine MH - *Urinary Bladder Neoplasms/diagnostic imaging/pathology MH - Random Forest OTO - NOTNLM OT - Biparametric magnetic resonance imaging OT - Habitat OT - Non-muscle-invasive bladder cancer OT - Risk stratification COIS- Declarations. Ethics approval and consent to participate: This retrospective study was approved by the Ethics Committee of the First Affiliated Hospital of Fujian Medical University. The study was conducted in accord with the Helsinki Declaration. Written informed consent was waived by the committee owing to the retrospective nature of the study and the use of de-identified archived clinical data. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:47 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/06/03 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/30 00:47 [entrez] AID - 10.1186/s12880-026-02630-7 [pii] AID - 10.1186/s12880-026-02630-7 [doi] PST - epublish SO - BMC Med Imaging. 2026 Aug 3;26(1):563. doi: 10.1186/s12880-026-02630-7. PMID- 42958092 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2334 (Electronic) IS - 1471-2334 (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Serum calpain-5 as a biomarker for disease severity and clinical outcomes in COVID-19 patients. LID - 1903 [pii] LID - 10.1186/s12879-026-14115-7 [doi] AB - BACKGROUND: Calpain is one of the intracellular calcium-activated cysteine proteases and exerts pivotal functions in many diseases. The aim was to explore the relationship of serum calpain-5 concentration and Coronavirus disease 2019 (COVID-19) via a cohort study. METHODS: The whole 338 COVID-19 patients were selected. Clinical features and demographic information were collected. The concentration of serum calpain-5 was measured via enzyme-linked immunosorbent assay (ELISA). RESULTS: Serum calpain-5 concentration was upregulated with increased severity scores, including SMART-COP, 4 C-Mortality, CURXO, A-DROP, DTPNCP, COVID-GRAM, PSI, and CURB-65 in COVID-19 patients. Spearman correlation analyses suggested there were tight correlations between serum calpain-5 level and numbers of clinical features. Additionally, multivariate linear and logistic regression models showed that serum calpain-5 level was positively associated with severity scores. Moreover, the frequencies of mechanical ventilation, vasoactive agent usage, ICU admission, and death during hospitalization within 30 days were grown with elevated serum calpain-5 concentration on admission. Logistic regression analyses demonstrated that serum higher calpain-5 on admission elevated the risk of adverse prognosis during hospitalization within 30 days. CONCLUSIONS: The concentration of serum calpain-5 is positively correlated with disease severity and adverse prognosis, implying calpain-5 may be involved in the progression of COVID-19. Serum calpain-5 level may served as a useful biomarker for guiding clinicians in managing and monitoring disease outcome in COVID-19 patients. CLINICAL TRIAL REGISTRATION: Clinical trial number: Not applicable. CI - (c) 2026. The Author(s). FAU - Miao, Hai-Long AU - Miao HL AD - School of Nursing, Anhui Medical University, Meishan Road 81, Hefei, Anhui, 230032, China. AD - Nursing Department, Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu, 225012, China. FAU - Liu, Meng-Xue AU - Liu MX AD - School of Nursing, Anhui Medical University, Meishan Road 81, Hefei, Anhui, 230032, China. FAU - Cao, Wei AU - Cao W AD - Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Anhui Medical University, Furong Road 678, Hefei, Anhui, 230601, China. FAU - Wu, Guo-Qiang AU - Wu GQ AD - School of Nursing, Anhui Medical University, Meishan Road 81, Hefei, Anhui, 230032, China. FAU - Li, Luo AU - Li L AD - Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Anhui Medical University, Furong Road 678, Hefei, Anhui, 230601, China. FAU - Cai, Xiang AU - Cai X AD - Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Anhui Medical University, Furong Road 678, Hefei, Anhui, 230601, China. FAU - Wang, Si-Han AU - Wang SH AD - Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Anhui Medical University, Furong Road 678, Hefei, Anhui, 230601, China. FAU - Fu, Lin AU - Fu L AD - Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Anhui Medical University, Furong Road 678, Hefei, Anhui, 230601, China. fulindev@126.com. FAU - Fei, Jun AU - Fei J AD - Department of Respiratory and Critical Care Medicine, Second Affiliated Hospital of Anhui Medical University, Furong Road 678, Hefei, Anhui, 230601, China. efy121409@fy.ahmu.edu.cn. FAU - Zhang, Fang AU - Zhang F AD - School of Nursing, Anhui Medical University, Meishan Road 81, Hefei, Anhui, 230032, China. 2009500029@ahmu.edu.cn. LA - eng GR - 82270071/National Natural Science Foundation of China/ GR - 2024zhyx-hl-B20/Doctor Research Project of the First Affiliated Hospital of Anhui Medical University/ GR - 2024xjxm76/Teaching Project/ PT - Journal Article DEP - 20260731 PL - England TA - BMC Infect Dis JT - BMC infectious diseases JID - 100968551 RN - EC 3.4.22.- (Calpain) RN - 0 (Biomarkers) SB - IM MH - Humans MH - *Calpain/blood MH - Male MH - Female MH - Biomarkers/blood MH - Middle Aged MH - Severity of Illness Index MH - *COVID-19/blood MH - Aged MH - SARS-CoV-2 MH - Prognosis MH - Adult MH - Cohort Studies MH - *Coronavirus Infections/blood MH - Betacoronavirus MH - Pandemics OTO - NOTNLM OT - COVID-19 OT - Calpain-5 OT - Clinical outcomes OT - Cohort study OT - Disease severity COIS- Declarations. Ethics approval and consent to participate: This study was supported by the Ethics Committee of Second Affiliated Hospital of Anhui Medical University (YJ-YX2021-147) and was conducted in accordance with the ethical standards of the Declaration of Helsinki. Written informed consent was obtained from all participants. Consent for publication: Not applicable. This study does not contain any individual-level identifiable data. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:47 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/01/03 00:00 [received] PHST- 2026/07/23 00:00 [accepted] PHST- 2026/10/30 00:47 [entrez] AID - 10.1186/s12879-026-14115-7 [pii] AID - 10.1186/s12879-026-14115-7 [doi] PST - epublish SO - BMC Infect Dis. 2026 Jul 31;26(1):1903. doi: 10.1186/s12879-026-14115-7. PMID- 42958086 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2342 (Electronic) IS - 1471-2342 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Ki-67/p53-associated angiogenic heterogeneity for preoperative WHO grading of meningiomas: a spatial habitat radiomics study. LID - 562 [pii] LID - 10.1186/s12880-026-02632-5 [doi] AB - PURPOSE: This study aimed to develop and validate a classification model for preoperative prediction of WHO grading of meningioma based on spatial habitat radiomics that can be explained by Ki-67 and p53 biomarkers. METHODS: A retrospective cohort of 400 patients (261 WHO grade I; 139 grade II/III) from a single institution was analyzed. Contrast-enhancing tumors were partitioned into three biologically distinct subregions (hypo-, intermediate-, and hyper-enhancement) using a 3D Simple Linear Iterative Clustering algorithm. Radiomics and clinical-radiological features were selected based on dual associations with Ki-67 >/= 5% and p53 >/= 5%. Subsequently, three classification models for preoperative prediction of WHO grading were constructed: clinical-radiological model (C-model), whole-tumor radiomics model (CRW-model), and subregion-based radiomics model (CRS-model). Performance was evaluated using AUC in training (n = 280) and test (n = 120) cohorts. RESULTS: Spatial habitat radiomics demonstrated biomarker-specific predictive power: hyper-enhancement subregions achieved the highest Ki-67 prediction AUC (training: 0.81; test: 0.70), while hypo-enhancement subregions excelled in p53 prediction (training: 0.93; test: 0.57). The CRS-model, achieved the highest AUC in both the training cohort (0.89 vs. 0.80 for CRW-model and 0.76 for C-model) and the test cohort (0.76 vs. 0.68 and 0.67, respectively). In the training cohort, the CRS-model significantly outperformed both the CRW-model and C-model after Bonferroni correction (adjusted p = 0.000450 and 7.06 x 10(-)(7), respectively). In the test cohort, the CRS-model showed numerically higher AUCs, but the differences were not statistically significant after correction (adjusted p = 0.0891 and 0.0918, respectively). CONCLUSIONS: Spatial habitat radiomics may help characterize intratumoral microenvironmental heterogeneity associated with Ki-67 and p53 expression, while providing a biologically interpretable framework for preoperative WHO grading of meningiomas. CI - (c) 2026. The Author(s). FAU - Xie, Huanhuan AU - Xie H AD - Department of Radiology, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. FAU - Peng, Xiting AU - Peng X AD - Department of Radiology, Taizhou Municipal Hospital, Taizhou, China. FAU - Chen, Xingzhi AU - Chen X AD - Deepwise AI Lab, Beijing Deepwise & League of PHD Technology Co., Ltd, Beijing, China. FAU - Yu, Yanting AU - Yu Y AD - Department of Radiology, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. AD - Department of Radiology, The Central Hospital Affiliated to Shaoxing University, Shaoxing Central Hospital), Shaoxing, China. FAU - Yi, Zhang AU - Yi Z AD - Department of Radiology, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. FAU - Meng, Wei AU - Meng W AD - Department of Radiology, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. FAU - Huang, Chencui AU - Huang C AD - Deepwise AI Lab, Beijing Deepwise & League of PHD Technology Co., Ltd, Beijing, China. FAU - You, Yi AU - You Y AD - Deepwise AI Lab, Beijing Deepwise & League of PHD Technology Co., Ltd, Beijing, China. FAU - Dong, Fei AU - Dong F AD - Department of Radiology, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. FAU - Zhang, Minming AU - Zhang M AD - Department of Radiology, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. FAU - Guan, Xiaojun AU - Guan X AD - Department of Radiology, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. xiaojunguan1102@zju.edu.cn. FAU - Xu, Xiaojun AU - Xu X AD - Department of Radiology, the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. xxjmailbox@zju.edu.cn. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - BMC Med Imaging JT - BMC medical imaging JID - 100968553 RN - 0 (Ki-67 Antigen) RN - 0 (Tumor Suppressor Protein p53) RN - 0 (Biomarkers, Tumor) RN - 0 (TP53 protein, human) SB - IM MH - Humans MH - Radiomics MH - *Ki-67 Antigen/metabolism MH - *Tumor Suppressor Protein p53/metabolism MH - *Meningioma/diagnostic imaging/pathology/metabolism MH - Female MH - Neoplasm Grading MH - Retrospective Studies MH - Male MH - Middle Aged MH - *Meningeal Neoplasms/diagnostic imaging/pathology/metabolism MH - Adult MH - Aged MH - Magnetic Resonance Imaging MH - Biomarkers, Tumor/metabolism MH - World Health Organization MH - Neovascularization, Pathologic/diagnostic imaging MH - Algorithms OTO - NOTNLM OT - Ki-67 OT - Meningioma grading OT - Radiomics OT - Spatial habitat analysis OT - Tumor heterogeneity OT - p53 COIS- Declarations. Ethics approval and consent to participate: The studies involving human participants were reviewed and approved by the Medical Ethics Committee of Second Affiliated Hospital of Zhejiang University School of Medicine. Written informed consent for participation of the retrospective study was not required in accordance with the national legislation and the institutional requirements. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:47 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/08/10 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/30 00:47 [entrez] AID - 10.1186/s12880-026-02632-5 [pii] AID - 10.1186/s12880-026-02632-5 [doi] PST - epublish SO - BMC Med Imaging. 2026 Aug 3;26(1):562. doi: 10.1186/s12880-026-02632-5. PMID- 42958080 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2318 (Electronic) IS - 1471-2318 (Linking) VI - 26 IP - 1 DP - 2026 Aug 1 TI - Associations between health literacy and selected lifestyle behaviors among community-dwelling older adults. LID - 1363 [pii] LID - 10.1186/s12877-026-08077-3 [doi] AB - OBJECTIVE: This study aimed to examine health literacy levels among community-dwelling older adults and to investigate the associations between health literacy and selected health-related behaviors, including smoking and physical inactivity. SUBJECT AND METHODS: Between August 2019 and July 2020, a community-based survey was undertaken in Balikesir, Turkey, involving residents aged 65 years and older living within the city center. Using Epi Info version 7.2, the minimum required sample size was calculated as 1,071, and 1,094 participants were ultimately recruited using multistage sampling. To evaluate the participants' health literacy capacities, THLS-32 was utilized alongside a structured questionnaire gathering sociodemographic details. Associations were examined using Generalized Estimating Equations (GEE) with cluster-adjusted robust standard errors. RESULTS: Overall, 33.8% of participants had problematic-limited / inadequate health literacy. In multivariable GEE analyses, participants aged >/= 80 years had higher odds of problematic-limited / inadequate health literacy than those aged 65-79 years (OR = 1.70, 95% CI: 1.02-2.85, p = 0.042). Moderate / poor perceived health was also associated with higher odds of problematic-limited / inadequate health literacy (OR = 1.93, 95% CI: 1.26-2.95, p = 0.002), whereas absence of chronic disease was associated with higher odds of problematic-limited / inadequate health literacy (OR = 2.04, 95% CI: 1.39-3.03, p < 0.001). No statistically significant association was observed between health literacy and smoking in the adjusted model. Smoking was independently associated with younger age (65-79 years: OR = 6.00, 95% CI: 2.50-14.37, p < 0.001) and male gender (OR = 5.59, 95% CI: 3.23-9.64, p < 0.001). Physical inactivity was independently associated with problematic-limited / inadequate HL (OR = 6.04, 95% CI: 3.62-10.10, p < 0.001), older age (OR = 2.83, 95% CI: 1.34-5.98, p = 0.007), income equal to or lower than expenditure (OR = 2.90, 95% CI: 1.27-6.62, p = 0.011), absence of chronic disease (OR = 1.97, 95% CI: 1.07-3.63, p = 0.030), and moderate/poor health perception (OR = 2.58, 95% CI: 1.56-4.26, p < 0.001). CONCLUSION: Approximately one-third of older adults had problematic-limited / inadequate health literacy. An independent association was observed between limited HL and a higher probability of being physically inactive. However, health literacy was not independently related to smoking. These findings imply that health literacy may be an important factor underlying differences in physical activity engagement among older adults. Community-based strategies integrating health literacy support and promotion of physical activity may contribute to healthy ageing. CI - (c) 2026. The Author(s). FAU - Cevik, Celalettin AU - Cevik C AUID- ORCID: 0000-0002-1123-6196 AD - Department of PHN, Faculty of Health Sciences, Balikesir University, Balikesir, Turkey. celalettincevik@balikesir.edu.tr. FAU - Simsek, Hatice AU - Simsek H AUID- ORCID: 0000-0001-7209-485X AD - Department of PH, Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey. FAU - Kayabek, Ibrahim AU - Kayabek I AUID- ORCID: 0000-0002-2005-2540 AD - Department of Nursing, Institute of Health Sciences, Balikesir University, Balikesir, Turkey. FAU - Dogan, Geylan AU - Dogan G AUID- ORCID: 0000-0002-7307-9974 AD - Department of Nursing, Institute of Health Sciences, Balikesir University, Balikesir, Turkey. FAU - Ay, Gizem AU - Ay G AUID- ORCID: 0000-0003-4520-4085 AD - Department of Nursing, Institute of Health Sciences, Balikesir University, Balikesir, Turkey. LA - eng GR - 2019/97/Balikesir Universitesi/ GR - 2019/97/Balikesir Universitesi/ GR - 2019/97/Balikesir Universitesi/ GR - 2019/97/Balikesir Universitesi/ GR - 2019/97/Balikesir Universitesi/ PT - Journal Article DEP - 20260801 PL - England TA - BMC Geriatr JT - BMC geriatrics JID - 100968548 SB - IM MH - Humans MH - Aged MH - Male MH - *Health Literacy/statistics & numerical data MH - Female MH - *Independent Living/psychology MH - Aged, 80 and over MH - *Life Style MH - *Health Behavior MH - Turkey/epidemiology MH - Cross-Sectional Studies MH - Smoking/epidemiology MH - Surveys and Questionnaires OTO - NOTNLM OT - Community-based study OT - Health literacy OT - Healthy lifestyle OT - Older adults OT - Physical inactivity OT - Smoking COIS- Declarations. Ethics approval and consent to participate: Financial support for this study was provided by the Balikesir University Scientific Research Projects Coordination Unit (Project No. 2019 / 57). Prior to data collection, all eligible participants were given detailed information regarding the purpose and procedures of the study. Verbal consent was then obtained from each participant. Individuals were assured that their involvement was entirely voluntary, that all information would be treated confidentially, and that they could discontinue participation at any point without any adverse consequences. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:47 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/10 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/30 00:47 [entrez] AID - 10.1186/s12877-026-08077-3 [pii] AID - 10.1186/s12877-026-08077-3 [doi] PST - epublish SO - BMC Geriatr. 2026 Aug 1;26(1):1363. doi: 10.1186/s12877-026-08077-3. PMID- 42958078 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2334 (Electronic) IS - 1471-2334 (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Clinical predictors, species distribution, and machine learning-based risk stratification of invasive candidiasis and in-hospital mortality: a retrospective episode-level cohort analysis. LID - 1900 [pii] LID - 10.1186/s12879-026-14105-9 [doi] AB - BACKGROUND: Invasive candidiasis carries disproportionate mortality in hospitalised patients, yet the clinical determinants distinguishing invasive from non-invasive disease remain inconsistently characterised across healthcare settings. This study aimed to identify independent predictors of invasive candidiasis and in-hospital mortality, describe species distribution and antifungal resistance patterns, and evaluate machine learning models for risk stratification. METHODS: A retrospective episode-level analysis was conducted on 206 laboratory-confirmed Candida-positive microbiological episodes at a single secondary care centre. Binary logistic regression univariable and multivariable was used to identify predictors of invasive candidiasis and all-cause mortality. Four machine learning classifiers (Random Forest, Gradient Boosting, Extra Trees, Logistic Regression) were developed and internally validated using five-fold stratified cross-validation. Permutation feature importance was applied for model explainability. RESULTS: Of 206 episodes, 37 (18.0%) were invasive. Overall, in-hospital mortality among invasive cases was 73.0%. Elderly age (>/= 65 years; aOR 53.22) and male sex (aOR 4.38) independently predicted invasive disease. Elderly age (aOR 5.12), male sex (aOR 4.96), and diabetes mellitus (aOR 2.60) were independent mortality predictors. Non-albicans species predominated among invasive isolates (73.0%), with Candida glabrata and Candida parapsilosis most over-represented. Fluconazole and caspofungin resistance were detected in 3/20 (15.0%) and 2/21 (9.5%) tested invasive isolates, respectively. The Random Forest classifier achieved AUROC values of 0.987 and 0.776 for invasive candidiasis and mortality prediction, respectively; the near-perfect invasive-candidiasis performance should be interpreted as exploratory and largely driven by marked age-based separation. CONCLUSION: Elderly age, male sex, and diabetes mellitus consistently predicted invasive disease and/or mortality in this episode-level cohort. Machine learning identified these routinely available variables as dominant discriminative features, but the models remain exploratory and require patient-level and external validation before clinical deployment. CI - (c) 2026. The Author(s). FAU - Sannathimmappa, Mohan Bilikallahalli AU - Sannathimmappa MB AUID- ORCID: 0000-0002-3429-7004 AD - Department of Microbiology, College of Medicine and Health Sciences, National University of Science and Technology, Sohar, Oman. drmohan123@gmail.com. FAU - Nambiar, Vinod AU - Nambiar V AD - Department of Microbiology, College of Medicine and Health Sciences, National University of Science and Technology, Sohar, Oman. FAU - Alsharawy, Mohammmed Al Amin Ali AU - Alsharawy MAAA AD - Department of Microbiology, College of Medicine and Health Sciences, National University of Science and Technology, Sohar, Oman. FAU - Baig, Motassim Kamran AU - Baig MK AD - Department of Microbiology, College of Medicine and Health Sciences, National University of Science and Technology, Sohar, Oman. FAU - Mahmood, Hussein Muqdad AU - Mahmood HM AD - Department of Microbiology, College of Medicine and Health Sciences, National University of Science and Technology, Sohar, Oman. FAU - Al-Shamaa, Ahmed Ali Abudlamir AU - Al-Shamaa AAA AD - Department of Microbiology, College of Medicine and Health Sciences, National University of Science and Technology, Sohar, Oman. FAU - Alabri, Miysaa Ali Abdallah AU - Alabri MAA AD - Department of Microbiology, College of Medicine and Health Sciences, National University of Science and Technology, Sohar, Oman. FAU - Almaskri, Rayyan Mohammed AU - Almaskri RM AD - Department of Microbiology, College of Medicine and Health Sciences, National University of Science and Technology, Sohar, Oman. FAU - Aravindakshan, Rajeev AU - Aravindakshan R AD - All India Institute of Medical Sciences, Mangalagiri, Hyderabad, India. FAU - Al-Maqbali, Salima AU - Al-Maqbali S AD - Department of Pathology and Blood Bank, Sohar Hospital, Sohar, Oman. FAU - Satti, Shahenaz AU - Satti S AD - Department of Physiology, Faculty of Medicine, Neelain University, Khartoum city, AL, Sudan. Shahenazsatti@gmail.com. FAU - Chowdhury, Rayaan AU - Chowdhury R AD - Department of Microbiology, College of Medicine and Health Sciences, National University of Science and Technology, Sohar, Oman. LA - eng PT - Journal Article DEP - 20260731 PL - England TA - BMC Infect Dis JT - BMC infectious diseases JID - 100968551 RN - 0 (Antifungal Agents) SB - IM MH - Humans MH - *Candidiasis, Invasive/mortality/microbiology/drug therapy/epidemiology MH - Retrospective Studies MH - Male MH - *Machine Learning MH - Female MH - *Hospital Mortality MH - *Candida/drug effects/classification/isolation & purification MH - Aged MH - Antifungal Agents/pharmacology/therapeutic use MH - Random Forest MH - Middle Aged MH - Aged, 80 and over MH - Risk Factors MH - Classification Algorithms MH - Predictive Learning Models MH - Logistic Models MH - Risk Assessment MH - Adult OTO - NOTNLM OT - Antifungal susceptibility OT - Diabetes mellitus OT - Elderly OT - Invasive candidiasis OT - Logistic regression OT - Machine learning OT - Mortality predictors OT - Random Forest OT - Risk stratification COIS- Declarations. Ethics approval and consent to participate: This study was approved by the institutional review board and ethical committee, Centre for Research Studies, Oman (approval number: MOH/CSR/23/27709; date: 29/11/2023), and was conducted in accordance with the ethical principles of the Declaration of Helsinki. Because this was a retrospective analysis of routinely collected de-identified clinical and microbiological data with no patient contact or intervention, the requirement for informed consent was waived by the approving committee. Consent for publication: Not applicable. AI declaration: An artificial intelligence-based language model was used only for language editing, readability, flow, and grammar support. All AI-assisted text was reviewed, revised, and approved by the authors, who take full responsibility for the final content. Some figures were prepared with AI-assisted graphical support and were checked by the authors for accuracy. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:47 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/04 00:00 [received] PHST- 2026/07/23 00:00 [accepted] PHST- 2026/10/30 00:47 [entrez] AID - 10.1186/s12879-026-14105-9 [pii] AID - 10.1186/s12879-026-14105-9 [doi] PST - epublish SO - BMC Infect Dis. 2026 Jul 31;26(1):1900. doi: 10.1186/s12879-026-14105-9. PMID- 42958075 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2288 (Electronic) IS - 1471-2288 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Effectiveness of recruitment methods in cancer clinical trials: an Australian experience. LID - 233 [pii] LID - 10.1186/s12874-026-02948-4 [doi] AB - OBJECTIVES: High-quality clinical research relies on the adequate recruitment of trial participants. The success of this underpins Evidence-Based Medicine. Trials participation is recommended however few patients have the opportunity to participate. The purpose of this study was to evaluate completed trials with the same sponsor to identify, define and evaluate various sponsor-led methods that can be applied to improve recruitment. METHODS: Building upon the Theories of Evidence-Based Medicine and Implementation Research, this study evaluated published data with the same Australian academic trial sponsor to identify, define and evaluate recruitment methods used in completed trials. Methods were ranked using a novel scale. Ethical and patient activation timeliness were considered. Data was extracted and tabulated (grouped, deidentified). Using descriptive statistics, a multi-disciplinary team synthesised and interpreted the data. RESULTS: Between 2008-2020, 56 trials sponsored by the Australian skin cancer network were screened; three studies met the criteria. We identified, described and ranked four recruitment methods (Trial design, Trial promotion, education and training, Site selection and feasibility, Trial resourcing and incentivization, supported by 27 specific initiatives). Timely ethics administration was shown to be important. Despite some methods being more effortful than others, all methods contributed patients. There are a number of study limitations. CONCLUSIONS: Clinical trials are complex to run. Insufficient accrual is commonplace, including for Australian skin cancer trials. Four distinct recruitment methods with examples were identified. The results of this study may help trial sponsors and any other researcher to improve trial recruitment and completion, as well as patient outcomes. CI - (c) 2026. The Author(s). FAU - Paton, Elizabeth J AU - Paton EJ AUID- ORCID: 0000-0002-7698-3248 AD - Faculty of Health, University of Technology Sydney, Ultimo, NSW, 2007, Australia. Elizabeth.j.paton@student.uts.edu.au. FAU - Shannon, Anthony G AU - Shannon AG AD - Faculty of Health, University of Technology Sydney, Ultimo, NSW, 2007, Australia. AD - University of Notre Dame Australia, School of Education, Broadway, NSW, 2007, Australia. AD - Australian Institute of Technology and Commerce, Haymarket, NSW, 2000, Australia. FAU - Fogarty, Gerald B AU - Fogarty GB AD - Faculty of Health, University of Technology Sydney, Ultimo, NSW, 2007, Australia. AD - University of Notre Dame Australia, School of Education, Broadway, NSW, 2007, Australia. AD - Faculty of Medicine, University of Notre Dame Australia, Darlinghurst, NSW, 2010, Australia. AD - Icon Cancer Centre, Sydney Adventist Hospital, Wahroonga, NSW, 2076, Australia. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - BMC Med Res Methodol JT - BMC medical research methodology JID - 100968545 SB - IM MH - Humans MH - *Patient Selection MH - Australia MH - *Clinical Trials as Topic/methods MH - *Neoplasms/therapy MH - Evidence-Based Medicine/methods MH - Research Design OAB - Building upon implementation research methods using the Consolidated Framework of Implementation Research in the clinical trial context, this novel study design evaluates outcomes from completed skin cancer trials conducted by a single sponsor. The study identified and prioritised four key recruitment domains - Trial design; Site selection and feasibility; Trial promotion, education and training; and Trial resourcing and incentivization - comprising 27 distinct initiatives that sponsors may consider when planning trials to enhance recruitment. A new metric was designed and evaluated to assess the real-world effort, impact, and relative importance of each method. Results suggest that trial sponsors should design a tailored, multi-faceted recruitment methodology that is aligned with the principal investigator and the study-specific needs early and throughout the trial lifecycle. OABL- eng OTO - NOTNLM OT - Australia OT - Evidence-based medicine OT - Implementation research OT - Oncology OT - Patient recruitment COIS- Declarations. Ethics approval and consent to participate: This study did not require approval from a human research ethics committee, as all data analysed were derived from publicly available sources and did not involve interaction with or the collection of identifiable personal information from human participants. Human research ethics committee approval was in place for each (funded, active) clinical trial that was considered when preparing the data for this study and any information was available in the public domain on the relevant Clinical Trial Registries. Consent for publication: Not applicable. There is no information in this study that could lead to the identification of study participants. The authors hereby consent for publication of the manuscript. Competing interests: Prof. Gerald B. Fogarty OAM is on the advisory boards of Merck Serono Australia Pty Ltd, La Roche-Posay, Sanofi-Aventis Australia Pty Ltd and Atomic Oncology Pty Ltd. He has received grants from Merck Serono Australia Pty Ltd, Sanofi-Aventis Australia Pty Ltd, OncoBeta GmBH and is an Icon Cancer Group shareholder. The other authors declare that are no conflict of interest. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:47 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/24 00:00 [received] PHST- 2026/07/09 00:00 [accepted] PHST- 2026/10/30 00:47 [entrez] AID - 10.1186/s12874-026-02948-4 [pii] AID - 10.1186/s12874-026-02948-4 [doi] PST - epublish SO - BMC Med Res Methodol. 2026 Aug 3;26(1):233. doi: 10.1186/s12874-026-02948-4. PMID- 42958073 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2334 (Electronic) IS - 1471-2334 (Linking) VI - 26 IP - 1 DP - 2026 Aug 1 TI - Vaccination and hybrid immunity is associated with SARS-CoV-2 protection but not shedding duration in rural Guatemalan households. LID - 1906 [pii] LID - 10.1186/s12879-026-14053-4 [doi] AB - BACKGROUND: Essential agricultural workers in Central America and their households faced a high force of infection during the COVID-19 pandemic. Here, we evaluated if pre-existing seropositivity and vaccination (hybrid immunity) influenced SARS-CoV-2 infection risk and duration of shedding in an agricultural community in rural Guatemala. METHODS: Individuals from 70 households were enrolled in a community cohort. From September 2021 to December 2022, all participants reported symptoms twice-weekly and provided saliva weekly for SARS-CoV-2 RNA testing by reverse transcriptase polymerase chain reaction (RT-PCR). Following detection of SARS-CoV-2 in a household, all enrolled household members provided saliva tested three times weekly for four weeks for RT-PCR testing to determine the duration of viral shedding among infected participants. We selected 119 SARS-CoV-2 PCR-positive individuals and 101 month-matched controls to measure nucleocapsid IgG and Omicron neutralizing antibody (NAb) titers. RESULTS: Compared to uninfected controls, SARS-CoV-2 cases were more frequently female (60.5% vs. 48.5%, p = 0.08), had fewer vaccine doses (p = 0.07), and were less likely to have hybrid immunity (p = 0.08). Compared to controls, SARS-CoV-2 cases had lower median titers of nucleocapsid IgG (cutoff index [COI] = 9.6 vs. 23.6, p = 0.12) and Omicron NAb (28.4 vs. 50.4, p = 0.15). SARS-CoV-2 cases also had lower NAb than controls among those vaccinated with 1 (16.1 vs. 182.7, p = 0.01), 2 (11.9 vs. 87.0, p = 0.04), or 3 doses (13.4 vs. 232.5, p = 0.004), and those considered up-to-date on vaccination (13.1 vs. 155.6, p = 0.0001). Nucleocapsid IgG and Omicron NAb were similar between individuals with short ( 7 days) viral shedding durations. CONCLUSIONS: In a household cohort of Guatemalan agricultural workers, cumulative vaccine doses associated with lower odds of infection. Among those previously vaccinated, lower titers of nucleocapsid IgG and Omicron NAb were associated with elevated infection risk, highlighting the importance of hybrid immunity in COVID-19 protection in this population. CI - (c) 2026. The Author(s). FAU - Barrett, Bradley S AU - Barrett BS AD - Division of Infectious Diseases, Department of Medicine, University of Colorado School of Medicine, Aurora, CO, USA. FAU - Lamb, Molly M AU - Lamb MM AD - Department of Epidemiology and Center for Global Health, Colorado School of Public Health, 13199 East Montview Blvd, Aurora, CO, USA. FAU - Guo, Kejun AU - Guo K AD - Division of Infectious Diseases, Department of Medicine, University of Colorado School of Medicine, Aurora, CO, USA. FAU - Calvimontes Barrientos, Diva Mirella AU - Calvimontes Barrientos DM AD - Center for Human Development, Fundacion para la Salud Integral de los Guatemaltecos, FSIG, Retalhuleu, Guatemala. AD - La Comision Presidencial de Atencion a la Emergencia COVID-19 (Coprecovid), Guatemala City, Guatemala. FAU - Rojop, Neudy AU - Rojop N AD - Center for Human Development, Fundacion para la Salud Integral de los Guatemaltecos, FSIG, Retalhuleu, Guatemala. FAU - Bradley, Claire AU - Bradley C AD - Center for Human Development, Fundacion para la Salud Integral de los Guatemaltecos, FSIG, Retalhuleu, Guatemala. FAU - Anabtawi, Hashem AU - Anabtawi H AD - Division of Infectious Diseases, Department of Medicine, University of Colorado School of Medicine, Aurora, CO, USA. FAU - Arias, Kareen AU - Arias K AD - Center for Human Development, Fundacion para la Salud Integral de los Guatemaltecos, FSIG, Retalhuleu, Guatemala. FAU - Paiz, Claudia AU - Paiz C AD - Center for Human Development, Fundacion para la Salud Integral de los Guatemaltecos, FSIG, Retalhuleu, Guatemala. FAU - Del Cid-Villatoro, Julio AU - Del Cid-Villatoro J AD - Center for Human Development, Fundacion para la Salud Integral de los Guatemaltecos, FSIG, Retalhuleu, Guatemala. FAU - Asturias, Edwin J AU - Asturias EJ AD - Department of Epidemiology and Center for Global Health, Colorado School of Public Health, 13199 East Montview Blvd, Aurora, CO, USA. AD - Center for Human Development, Fundacion para la Salud Integral de los Guatemaltecos, FSIG, Retalhuleu, Guatemala. AD - Division of Infectious Disease, Department of Pediatrics, University of Colorado School of Medicine, 13123 E. 16th Ave., Aurora, CO, USA. FAU - Santiago, Mario L AU - Santiago ML AD - Division of Infectious Diseases, Department of Medicine, University of Colorado School of Medicine, Aurora, CO, USA. mario.santiago@cuanschutz.edu. FAU - Olson, Daniel AU - Olson D AUID- ORCID: 0000-0002-2374-1448 AD - Department of Epidemiology and Center for Global Health, Colorado School of Public Health, 13199 East Montview Blvd, Aurora, CO, USA. Daniel.Olson@cuanschutz.edu. AD - Center for Human Development, Fundacion para la Salud Integral de los Guatemaltecos, FSIG, Retalhuleu, Guatemala. Daniel.Olson@cuanschutz.edu. AD - Division of Infectious Disease, Department of Pediatrics, University of Colorado School of Medicine, 13123 E. 16th Ave., Aurora, CO, USA. Daniel.Olson@cuanschutz.edu. LA - eng PT - Journal Article DEP - 20260801 PL - England TA - BMC Infect Dis JT - BMC infectious diseases JID - 100968551 RN - 0 (Antibodies, Viral) RN - 0 (Antibodies, Neutralizing) RN - 0 (COVID-19 Vaccines) RN - 0 (Immunoglobulin G) SB - IM MH - Humans MH - Female MH - Guatemala/epidemiology MH - *COVID-19/immunology/prevention & control/epidemiology/virology MH - *SARS-CoV-2/immunology MH - Adult MH - Male MH - Antibodies, Viral/blood/immunology MH - Rural Population MH - Antibodies, Neutralizing/blood/immunology MH - Vaccination MH - *Virus Shedding/immunology MH - Middle Aged MH - *COVID-19 Vaccines/immunology/administration & dosage MH - Family Characteristics MH - Young Adult MH - Immunoglobulin G/blood OTO - NOTNLM OT - Agricultural workers OT - COVID-19 OT - Guatemala OT - Households OT - Neutralizing antibodies OT - Omicron OT - SARS-CoV-2 OT - Vaccine COIS- Declarations. Ethics approval and consent to participate: The study was carried out in accordance with the Declaration of Helsinki. The study was approved by the Colorado Multiple Institutional Review Board (COMIRB protocol #21-2551), the UVG Ethics Committee (#225-01-2021), and CDC (#212551) as human subjects research. The local SW Trifinio Community Advisory Board for Research agreed to the study. All study participants provided written informed consent before enrollment in the AGRI-CASA cohort. Consent included longitudinal collection, storage, and testing of clinical specimens for SARS-CoV-2 related research. The present nested case-control analysis used previously collected specimens and data from participants enrolled under this approved protocol. Consent for publication: Not applicable. Competing interests: The research presented in this manuscript was funded in part by Roche Diagnostics. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:47 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/07 00:00 [received] PHST- 2026/07/16 00:00 [accepted] PHST- 2026/10/30 00:47 [entrez] AID - 10.1186/s12879-026-14053-4 [pii] AID - 10.1186/s12879-026-14053-4 [doi] PST - epublish SO - BMC Infect Dis. 2026 Aug 1;26(1):1906. doi: 10.1186/s12879-026-14053-4. PMID- 42958066 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2731-4553 (Electronic) IS - 2731-4553 (Linking) VI - 27 IP - 1 DP - 2026 Aug 3 TI - Community perceptions of primary health care in Oyo State, Nigeria: a cross-sectional study. LID - 393 [pii] LID - 10.1186/s12875-026-03508-x [doi] AB - BACKGROUND: Primary Health Care (PHC) is central to achieving equitable and cost-effective health systems, yet its potential in Nigeria remains underutilised. Despite the extensive network of PHC centres in Oyo State, utilisation remains low, partly due to misconceptions about PHC functions and varying community experiences. This study assessed awareness, perceptions, and predictors of PHC utilisation among residents of Oyo State. METHODS: A cross-sectional descriptive study was conducted between July and August 2025 across 16 purposively selected Local Government Areas (LGAs) in Oyo State to ensure urban-rural representation. Data were collected through online and interviewer-administered questionnaires. Descriptive statistics summarised sociodemographic characteristics and awareness patterns. Chi-square tests assessed associations between sociodemographic variables and PHC awareness. Logistic regression identified predictors of PHC utilisation, with significance set at p < 0.05. RESULTS: A total of 694 respondents participated. Overall, 80.9% reported awareness of a PHC centre in their locality, although awareness differed significantly by age (chi(2)=44.99, p < 0.001), marital status (chi(2)=29.77, p < 0.001), and number of children (chi(2)=9.69, p = 0.021). Knowledge of PHC functions revealed notable gaps. Routine immunisation (54.5%) and treatment of uncomplicated malaria (48.7%) were widely recognised, whereas awareness of emergency care (30.4%), hypertension management (15.0%), and diabetes care (6.8%) was low. In the multivariable model, older age groups showed reduced odds of PHC utilisation compared with those under 20 years, particularly respondents aged 31-40 years (AOR = 0.34; 95% CI: 0.16-0.75), 41-50 years (AOR = 0.22; 95% CI: 0.09-0.54), and 51-60 years (AOR = 0.32; 95% CI: 0.12-0.82). Married respondents were more likely to use PHC services (AOR = 3.26; 95% CI: 1.75-6.10). Higher income was associated with lower utilisation, especially among those earning above ₦300,000 (AOR = 0.25; 95% CI: 0.10-0.66). Awareness of PHC centres was the strongest predictor of utilisation (AOR = 7.12; 95% CI: 4.37-11.61). CONCLUSION: Although awareness of PHC centres is high, substantial gaps remain regarding the breadth of PHC services. Age, marital status, income, and PHC awareness significantly influenced utilisation. Strengthening health education and implementing targeted communication strategies, particularly for young adults, older persons, and higher-income earners, may enhance PHC uptake and contribute to more equitable utilisation of primary healthcare services in Oyo State. CI - (c) 2026. The Author(s). FAU - Akhiwu, Jecinta Seiyefa AU - Akhiwu JS AD - Department of Epidemiology and Medical Statistics, Faculty of Public Health, College of Medicine, University of Ibadan, Ibadan, Nigeria. FAU - Alonge, Temitope Oluwagbenga AU - Alonge TO AD - Department of Surgery, Faculty of Clinical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria. FAU - Okoroafor, Promise Izuchukwu AU - Okoroafor PI AUID- ORCID: 0009-0009-5037-8777 AD - Department of Epidemiology and Medical Statistics, Faculty of Public Health, College of Medicine, University of Ibadan, Ibadan, Nigeria. okoroaforizuchukwu@gmail.com. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - BMC Prim Care JT - BMC primary care JID - 9918300889006676 SB - IM MH - Humans MH - Cross-Sectional Studies MH - Nigeria MH - *Primary Health Care/statistics & numerical data MH - Female MH - Adult MH - Male MH - Middle Aged MH - *Health Knowledge, Attitudes, Practice MH - Adolescent MH - Young Adult MH - Surveys and Questionnaires MH - Socioeconomic Factors MH - *Patient Acceptance of Health Care/statistics & numerical data MH - Aged OTO - NOTNLM OT - Awareness OT - Community perception OT - Health service utilisation OT - Primary health care OT - Universal health coverage COIS- Declarations. Ethics approval and consent to participate: Ethical approval for this study was obtained from the University of Ibadan/University College Hospital Institutional Ethics Committee (Approval No: UI/EC/25/0318). All participants were informed about the objectives, procedures, potential risks, and voluntary nature of the study before data collection commenced. Written informed consent was obtained from every participant. For participants with limited literacy, the consent document was translated into Yoruba, the predominant local language, and interviewers provided verbal explanations to ensure full comprehension before obtaining consent. The questionnaire was interviewer-administered in such cases to support accurate and meaningful participation. Confidentiality was strictly maintained by ensuring that no personally identifiable information was collected. All procedures followed the ethical principles outlined in the Declaration of Helsinki. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:46 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/11/28 00:00 [received] PHST- 2026/07/31 00:00 [accepted] PHST- 2026/10/30 00:46 [entrez] AID - 10.1186/s12875-026-03508-x [pii] AID - 10.1186/s12875-026-03508-x [doi] PST - epublish SO - BMC Prim Care. 2026 Aug 3;27(1):393. doi: 10.1186/s12875-026-03508-x. PMID- 42958065 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2342 (Electronic) IS - 1471-2342 (Linking) VI - 26 IP - 1 DP - 2026 Aug 1 TI - An interpretable computed tomography radiomics-based machine learning model for preoperative prediction of complicated appendicitis in children. LID - 560 [pii] LID - 10.1186/s12880-026-02628-1 [doi] AB - BACKGROUND: Accurate preoperative discrimination between uncomplicated and complicated acute appendicitis in children is critical for optimal surgical decision-making but remains a clinical challenge. Current reliance on conventional imaging and laboratory findings lacks sufficient precision, necessitating the development of more reliable predictive tools. METHODS: We conducted a multicenter retrospective study to develop and validate a machine-learning model integrating CT radiomics and clinical variables. Data from 287 pediatric patients (aged 2-14 years) at Xiaogan Central Hospital (Jan 2017-May 2025) were randomly divided into a training (n = 201) and an internal-validation cohort (n = 86). An independent external-validation cohort comprised 164 patients from Yichang Central People's Hospital (Nov 2023-May 2025). Preoperative CT images and clinical data were processed; features with < 30% missing values were imputed. An automated modeling pipeline constructed 100 candidate models by combining 10 feature-selection algorithms with 10 machine-learning frameworks. Model performance was assessed using receiver operating characteristic curve analysis, with interpretability provided by SHapley Additive exPlanations (SHAP). RESULTS: The optimal model, chi square feature selection combined with AdaBoost, demonstrated robust discriminative ability. It achieved AUCs of 0.900 (95% CI: 0.832-0.958), 0.889 (95% CI: 0.841-0.937), and 0.893 (95% CI: 0.855-0.931) in the training, internal-validation, and external-validation cohorts, respectively. The model achieved a sensitivity of 87% and specificity of 85% in the external-validation cohort. SHAP analysis identified radiomic texture features (RootMeanSquared, Strength, Skewness), appendicolith presence, and maximum body temperature as the most important predictors. CONCLUSION: A CT radiomics-based machine learning model integrating clinical features can accurately differentiate complicated from uncomplicated appendicitis in children, providing an interpretable framework to support preoperative surgical decision-making. TRIAL REGISTRATION: Not applicable (retrospective diagnostic study). CI - (c) 2026. The Author(s). FAU - Li, Zhaopu AU - Li Z AD - Department of General Surgery, Xiaogan Hospital Affiliated to Wuhan University of Science and Technology, Xiaogan, Hubei, 432000, China. AD - Medical College, Wuhan University of Science and Technology, Wuhan, Hubei, 430065, China. FAU - Li, Yang AU - Li Y AD - Department of General Surgery, Xiaogan Hospital Affiliated to Wuhan University of Science and Technology, Xiaogan, Hubei, 432000, China. FAU - Zhang, Zhongjing AU - Zhang Z AD - Department of Pediatric Surgery, Yichang Central People's Hospital Affiliated to China Three Gorges University, Yichang, Hubei, 443003, China. FAU - Lin, Jiaqing AU - Lin J AD - Department of General Surgery, Xiaogan Hospital Affiliated to Wuhan University of Science and Technology, Xiaogan, Hubei, 432000, China. AD - Medical College, Wuhan University of Science and Technology, Wuhan, Hubei, 430065, China. FAU - Xu, Tao AU - Xu T AD - Department of General Surgery, Xiaogan Hospital Affiliated to Wuhan University of Science and Technology, Xiaogan, Hubei, 432000, China. AD - Medical College, Wuhan University of Science and Technology, Wuhan, Hubei, 430065, China. FAU - Zhu, Wei AU - Zhu W AD - Department of General Surgery, Xiaogan Hospital Affiliated to Wuhan University of Science and Technology, Xiaogan, Hubei, 432000, China. FAU - Guo, Zhaokun AU - Guo Z AD - Department of Pediatric Surgery, Yichang Central People's Hospital Affiliated to China Three Gorges University, Yichang, Hubei, 443003, China. 12893437@qq.com. FAU - Yang, Kun AU - Yang K AD - Department of General Surgery, Xiaogan Hospital Affiliated to Wuhan University of Science and Technology, Xiaogan, Hubei, 432000, China. yangkunmedical@163.com. LA - eng GR - XGKJ2023010016/Natural Science Foundation of Xiaogan Municipality/ PT - Journal Article PT - Multicenter Study DEP - 20260801 PL - England TA - BMC Med Imaging JT - BMC medical imaging JID - 100968553 SB - IM MH - Humans MH - *Radiomics MH - *Appendicitis/diagnostic imaging/surgery MH - *Tomography, X-Ray Computed/methods MH - Child MH - Child, Preschool MH - Retrospective Studies MH - Male MH - *Machine Learning MH - Adolescent MH - Female MH - Predictive Learning Models MH - ROC Curve OTO - NOTNLM OT - CT radiomics OT - Children OT - Complicated appendicitis OT - Machine learning COIS- Declarations. Ethics approval and consent to participate: This retrospective two-center cohort study was approved by the institutional review board of Xiaogan Central Hospital (XGCH; approval no. 2024012308) with a waiver of informed consent due to the retrospective nature of the study and the use of fully de-identified data. All data were de-identified in accordance with the Personal Information Protection Law of the People's Republic of China prior to analysis. Consent for publication: Not applicable. This manuscript does not contain any individual person's data in any form that would require consent for publication. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:46 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/12/08 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/30 00:46 [entrez] AID - 10.1186/s12880-026-02628-1 [pii] AID - 10.1186/s12880-026-02628-1 [doi] PST - epublish SO - BMC Med Imaging. 2026 Aug 1;26(1):560. doi: 10.1186/s12880-026-02628-1. PMID- 42958059 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2318 (Electronic) IS - 1471-2318 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Network analysis of frailty, sleep quality, and life satisfaction in older patients: a cross-sectional study stratified by chronic disease burden. LID - 1365 [pii] LID - 10.1186/s12877-026-07958-x [doi] AB - BACKGROUND: Multimorbidity and chronic disease burden are common among older patients and are closely associated with frailty, poor sleep, and reduced subjective well-being. However, total-score-based analyses may obscure item-level interactions across health domains. This study examined the network structure of frailty, sleep quality, and life satisfaction in older patients and explored whether association patterns varied across chronic disease burden levels. METHODS: This cross-sectional study included 1,107 older patients. Multimorbidity was defined as the presence of two or more chronic diseases. Frailty, sleep quality, and life satisfaction were assessed using the Fatigue, Resistance, Ambulation, Illnesses, and Loss of Weight (FRAIL) scale, Pittsburgh Sleep Quality Index (PSQI), and Satisfaction With Life Scale (SWLS), respectively. A regularized partial correlation network was estimated using the graphical least absolute shrinkage and selection operator (LASSO) with extended Bayesian information criterion (EBIC) model selection (EBICglasso), with scale items or components as nodes. Strength, expected influence, and bridge centrality were calculated. Stratified analyses were conducted by number of chronic diseases, and bootstrap procedures were used to assess network accuracy and stability. RESULTS: Multimorbidity was present in 1,078 participants (97.4%), and the median number of chronic diseases was 5 (IQR, 3-6). Network analysis showed that SWLS-related nodes had relatively high strength and bridge strength, indicating relatively high statistical connectivity within the overall network and across communities. Sleep-related nodes, particularly sleep latency, use of sleeping medication, and daytime dysfunction, were prominent in signed centrality indices. Frailty nodes were mainly connected to SWLS nodes through negative edges. The SWLS community showed dense internal connections, whereas frailty and sleep quality were linked to life satisfaction primarily through negative cross-community edges. In descriptive stratified analyses, negative cross-community connections appeared more pronounced in the group with >/= 5 chronic diseases. Because the cutoff was data-driven and no formal network comparison test was performed, the apparent between-group differences should be interpreted as descriptive and exploratory rather than statistically confirmed. Network stability analyses indicated acceptable stability. CONCLUSIONS: Among older patients, life satisfaction-related nodes showed relatively high overall connectivity, while several sleep-related nodes showed notable statistical associations within the estimated network. Given the high prevalence of multimorbidity in this sample, multidisciplinary assessment and health management should consider the interrelations among sleep problems, frailty, and life satisfaction in the context of chronic disease burden. Network analysis may provide exploratory evidence on cross-domain associations in complex health problems among older patients, but apparent differences across chronic disease burden groups require confirmation using formal network comparison tests and longitudinal designs. CI - (c) 2026. The Author(s). FAU - Wang, Zhiqiang AU - Wang Z AD - Health Science Center, Yangtze University, No. 1 Xueyuan Road, Jingzhou, Hubei, 434023, P.R. China. FAU - Wei, Zhanhua AU - Wei Z AD - Health Science Center, Yangtze University, No. 1 Xueyuan Road, Jingzhou, Hubei, 434023, P.R. China. AD - Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, No. 600 Yishan Road, Xuhui District, Shanghai, 200233, P.R. China. FAU - Li, Xiaoya AU - Li X AD - Health Science Center, Yangtze University, No. 1 Xueyuan Road, Jingzhou, Hubei, 434023, P.R. China. FAU - Hu, Mengqiu AU - Hu M AD - Health Science Center, Yangtze University, No. 1 Xueyuan Road, Jingzhou, Hubei, 434023, P.R. China. AD - Huanggang Central Hospital, No. 6 Qi'an Avenue, Huangzhou District, Huanggang, Hubei, 438000, P.R. China. FAU - Deng, Kaiqin AU - Deng K AD - Outpatient Service Center, Jingzhou First People's Hospital, No. 8 Hangkong Road, Shashi District, Jingzhou, Hubei, 434000, P.R. China. FAU - Cheng, Tong AU - Cheng T AD - Health Science Center, Yangtze University, No. 1 Xueyuan Road, Jingzhou, Hubei, 434023, P.R. China. FAU - Zhao, Limeng AU - Zhao L AD - Department of Geriatric Medicine, Xilingol League Central Hospital, Inner Mongolia Autonomous Region, No. 9 Nadamu Street, Xilinhot, 026000, P.R. China. FAU - Han, Xu AU - Han X AD - Department of Geriatric Medicine, Xilingol League Central Hospital, Inner Mongolia Autonomous Region, No. 9 Nadamu Street, Xilinhot, 026000, P.R. China. FAU - Liang, Zhiru AU - Liang Z AD - Department of Hepatobiliary Surgery, Xilingol League Central Hospital, Inner Mongolia Autonomous Region, No. 9 Nadamu Street, Xilinhot, 026000, P.R. China. liangzhiru999@163.com. FAU - Wang, Jing AU - Wang J AD - Department of Anesthesiology, Xilingol League Central Hospital, Inner Mongolia Autonomous Region, No. 9 Nadamu Street, Xilinhot, 026000, P.R. China. wangjing19940513@163.com. FAU - Zhao, Shuping AU - Zhao S AD - Department of Geriatric Medicine, Xilingol League Central Hospital, Inner Mongolia Autonomous Region, No. 9 Nadamu Street, Xilinhot, 026000, P.R. China. zhaoshuping2026@126.com. LA - eng PT - Journal Article DEP - 20260803 PL - England TA - BMC Geriatr JT - BMC geriatrics JID - 100968548 SB - IM MH - Humans MH - Cross-Sectional Studies MH - Female MH - Chronic Disease/psychology/epidemiology MH - Male MH - *Sleep Quality MH - *Frailty/psychology/epidemiology/diagnosis MH - Aged, 80 and over MH - Aged MH - *Personal Satisfaction MH - *Quality of Life/psychology MH - *Frail Elderly/psychology MH - Multimorbidity MH - *Cost of Illness OTO - NOTNLM OT - Chronic disease burden OT - Frailty OT - Life satisfaction OT - Multimorbidity OT - Network analysis OT - Older patients OT - Sleep quality COIS- Declarations. Ethics approval and consent to participate: This study was reviewed and approved by the Ethics Committee of Jingzhou First People's Hospital (approval No. YJ202563). The study was conducted in accordance with the Declaration of Helsinki. Informed consent was obtained from all participants before participation. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:46 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/05 00:00 [received] PHST- 2026/07/01 00:00 [accepted] PHST- 2026/10/30 00:46 [entrez] AID - 10.1186/s12877-026-07958-x [pii] AID - 10.1186/s12877-026-07958-x [doi] PST - epublish SO - BMC Geriatr. 2026 Aug 3;26(1):1365. doi: 10.1186/s12877-026-07958-x. PMID- 42958058 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-230X (Electronic) IS - 1471-230X (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Effects of bariatric surgery on non-invasive markers of MASLD: a retrospective analysis. LID - 711 [pii] LID - 10.1186/s12876-026-05187-5 [doi] AB - INTRODUCTION: MASLD is common form of chronic liver disease leading to cirrhosis and liver cancer. This study evaluated the impact of bariatric surgery on the progression of MASLD. METHODS: This was a retrospective study of 181 patients undergoing weight loss surgery (sleeve gastrectomy (SG = 54) or Roux-en-Y gastric bypass (RGB = 127) at TidalHealth, Salisbury, MD from 2017 to 22. Non-invasive markers of fibrosis: FIB-4, MASLD Fibrosis Score (MFS), and BARD were assessed at baseline and 12-18 months post-op. Change in fibrosis was assessed using the paired t-test. RESULTS: Baseline demographics, including BMI, AST, ALT, platelet count, and comorbidities (diabetes, hypertension), are presented in Table 1. Pre- and post-op changes in non-invasive fibrosis scores are summarized in Table 2. The majority of the included patients (127) underwent gastric bypass (70.2%); whereas 54 patients underwent a gastric sleeve procedure (29.8%). Mean change in BMI was 46.2 to 37.8 (p < 0.001). A statistically significant reduction in the mean MFS (0.205 to -0.373, p = 0.0001; 95% CI: 0.28 to 0.87) was observed, indicating a shift in fibrosis scores from the indeterminate to the low-risk fibrosis category for the entire cohort. In a subgroup of 25 patients with advanced baseline fibrosis (FIB-4 > 1.4[2] ), bariatric surgery resulted in a significant reduction in MFS from 1.63 to 0.84 (p = 0.001), indicative of meaningful score-based improvement in estimated fibrosis risk. In a subgroup of patients with advanced fibrosis at baseline (n = 25 patients), the drop in BARD score was statistically significant (3.48 to 3.28; p = 0.05). The FIB-4 score showed a modest but statistically significant increase in the overall cohort (0.95 to 1.02; p = 0.004), and remained essentially unchanged in the advanced fibrosis subgroup (1.94 to 1.92; p = 0.80), highlighting the limitations of this index for longitudinal monitoring. CONCLUSION: Bariatric surgery resulted in significant improvement in non-invasive fibrosis scores of MASLD, as shown by reduction in the MFS and BARD scores in the group with advanced fibrosis. Changes in the FIB-4 scores were less consistent, suggesting this test is less useful for longitudinal assessment of fibrosis in patients with MASLD and is better suited as a screening tool. CI - (c) 2026. The Author(s). FAU - Lukolic, Ismet AU - Lukolic I AD - Department of Gastroenterology, Tidal Health Peninsula Regional, Salisbury, MD, USA. FAU - Khan, Junaid AU - Khan J AUID- ORCID: 0009-0001-4828-9929 AD - Department of Internal Medicine, Tidal Health Peninsula Regional, 100 East Carroll Street, Salisbury, MD, 21801, USA. Junaid.akhanmd@gmail.com. FAU - McCabe, Michael AU - McCabe M AD - Department of Internal Medicine, Tidal Health Peninsula Regional, 100 East Carroll Street, Salisbury, MD, 21801, USA. FAU - Jibran, Muhammad AU - Jibran M AD - Department of Internal Medicine, Tidal Health Peninsula Regional, 100 East Carroll Street, Salisbury, MD, 21801, USA. FAU - Hamza, Mohammad AU - Hamza M AD - Department of Internal Medicine, Tidal Health Peninsula Regional, 100 East Carroll Street, Salisbury, MD, 21801, USA. FAU - Rojony, Rajoana AU - Rojony R AD - Department of Internal Medicine, Tidal Health Peninsula Regional, 100 East Carroll Street, Salisbury, MD, 21801, USA. FAU - Ismail, Safana AU - Ismail S AD - Department of Internal Medicine, Tidal Health Peninsula Regional, 100 East Carroll Street, Salisbury, MD, 21801, USA. FAU - Patel, Dhruvkumar AU - Patel D AD - Department of Gastroenterology, Tidal Health Peninsula Regional, Salisbury, MD, USA. FAU - Maheshwari, Anurag AU - Maheshwari A AD - Department of Gastroenterology & Hepatology, Mercy Medical Center, University of Maryland School of Medicine, Baltimore, USA. LA - eng PT - Journal Article DEP - 20260731 PL - England TA - BMC Gastroenterol JT - BMC gastroenterology JID - 100968547 RN - 0 (Biomarkers) SB - IM MH - Humans MH - Retrospective Studies MH - Female MH - Biomarkers/blood MH - *Bariatric Surgery/methods MH - Male MH - Middle Aged MH - Adult MH - *Liver Cirrhosis/blood MH - Disease Progression MH - *Obesity, Morbid/surgery/complications MH - Gastric Bypass MH - Body Mass Index MH - *Non-alcoholic Fatty Liver Disease/blood MH - Gastrectomy OTO - NOTNLM OT - BARD score OT - Bariatric surgery OT - FIB-4 score OT - MASLD OT - NAFLD Fibrosis Score COIS- Declarations. Ethics approval and consent to participate: This study was endorsed by the our institute Research Review Committee (RRC) on October 30, 2024 (Protocol P24-017), and subsequently submitted to WCG IRB (Western Institutional Review Board). On October 31, 2024, WCG IRB issued an exemption determination, classifying the study as exempt under 45 CFR section sign 46.104(d)(4), as it involved only retrospective analysis of de-identified patient data. The requirement for informed consent to participate was waived accordingly by WCG IRB. All procedures were performed in accordance with the ethical standards of the responsible institutional and independent committees and with the 1964 Declaration of Helsinki and its later amendments or comparable ethical standards. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:46 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/30 00:00 [received] PHST- 2026/07/29 00:00 [accepted] PHST- 2026/10/30 00:46 [entrez] AID - 10.1186/s12876-026-05187-5 [pii] AID - 10.1186/s12876-026-05187-5 [doi] PST - epublish SO - BMC Gastroenterol. 2026 Jul 31;26(1):711. doi: 10.1186/s12876-026-05187-5. PMID- 42958052 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2261 (Electronic) IS - 1471-2261 (Linking) VI - 26 IP - 1 DP - 2026 Aug 4 TI - Artificial intelligence-enabled non-invasive quantification of left ventricular end-systolic pressure: an in silico analysis. LID - 999 [pii] LID - 10.1186/s12872-026-06358-8 [doi] AB - BACKGROUND: Invasive pressure-volume loop (PVL) analysis is the gold standard method to quantify myocardial physiology. To enhance broader clinical applicability, non-invasive approaches to derive left ventricular (LV) PVLs have gained prominence, typically estimating LV end-systolic pressure (ESP) from brachial blood pressure (BP) alone. However, this approach does not account for factors that may modulate the pressure waveform as it propagates from the LV to the brachial artery, notably arterial stiffness. We trained an artificial-intelligence (AI) model to predict invasive LVESP from a set of non-invasive hemodynamic parameters and compared its performance with commonly used equations for estimating LVESP using solely brachial BPs. METHODS: An in silico cohort of healthy adults (N = 3,837, age: 48.6 +/- 17.0y), previously shown to have good agreement for aortic and brachial BP with in vivo data, was split into training and validation cohorts using an internal hold-out validation approach. Clinically relevant parameters were selected as inputs to a random forest machine learning model. RESULTS: The best performing model included systolic and diastolic brachial BP, heart rate, age, and carotid-femoral pulse wave velocity (PWV), and reliably predicted LVESP (root mean square error: 0.99 mmHg). AI-derived LVESP correlated more strongly with simulated invasive LVESP (r = 0.994) than commonly used equations (r = 0.909-0.908). Bland-Altman analysis demonstrated significant bias in brachial BP-only equations (mean bias: -4.01, limits of agreement [LoA] [+/- 1.96 standard deviations]: -11.1 to 3.06 mmHg), with the direction of bias varying across different ages. AI-derived LVESP displayed smaller bias (mean bias: 0.002, LoA: -1.94 to 1.94 mmHg) and had negligible age-related bias across the lifespan. CONCLUSIONS: In this hypothesis generating study, a novel non-invasive, AI-enabled approach to LVESP quantification incorporating age, PWV and other key hemodynamic parameters alongside brachial BPs yielded smaller average error across the lifespan than commonly used brachial BP-only equations. These results warrant validation in vivo to ascertain real-world predictive performance. CI - (c) 2026. The Author(s). FAU - Long, Edouard AU - Long E AUID- ORCID: 0009-0002-1008-7966 AD - Faculty of Life Sciences and Medicine, King's College London, London, UK. edouard.long@kcl.ac.uk. FAU - Dunn, Roberta AU - Dunn R AUID- ORCID: 0009-0003-7253-9128 AD - Faculty of Life Sciences and Medicine, King's College London, London, UK. FAU - Androshchuk, Vitaliy AU - Androshchuk V AUID- ORCID: 0000-0002-1464-3109 AD - Faculty of Life Sciences and Medicine, King's College London, London, UK. LA - eng GR - FS/CRTF/22/24328/BHF_/British Heart Foundation/United Kingdom PT - Comparative Study PT - Journal Article PT - Validation Study DEP - 20260804 PL - England TA - BMC Cardiovasc Disord JT - BMC cardiovascular disorders JID - 100968539 SB - IM MH - Humans MH - *Ventricular Function, Left MH - Predictive Value of Tests MH - Female MH - Middle Aged MH - Male MH - Adult MH - Reproducibility of Results MH - *Computer Simulation MH - Aged MH - *Models, Cardiovascular MH - *Arterial Pressure MH - *Ventricular Pressure MH - Brachial Artery MH - Pulse Wave Analysis MH - Predictive Learning Models MH - Vascular Stiffness MH - Carotid-Femoral Pulse Wave Velocity MH - *Blood Pressure Determination MH - Young Adult MH - Stroke Volume OTO - NOTNLM OT - Artificial intelligence OT - Blood pressure OT - Left ventricle COIS- Declarations. Ethics approval and consent to participate: Ethical approval for this study was not required as the dataset is publicly available and does not contain any patient-identifiable information. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:45 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/29 00:00 [received] PHST- 2026/07/23 00:00 [accepted] PHST- 2026/10/30 00:45 [entrez] AID - 10.1186/s12872-026-06358-8 [pii] AID - 10.1186/s12872-026-06358-8 [doi] PST - epublish SO - BMC Cardiovasc Disord. 2026 Aug 4;26(1):999. doi: 10.1186/s12872-026-06358-8. PMID- 42958042 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2229 (Electronic) IS - 1471-2229 (Linking) VI - 26 IP - 1 DP - 2026 Aug 1 TI - Development and validation of a KASP marker for a domestication-associated stem diameter allele in sorghum. LID - 1846 [pii] LID - 10.1186/s12870-026-09674-5 [doi] AB - Stem diameter is a key agronomic trait in sorghum (Sorghum bicolor L.), influencing biomass accumulation and stalk strength. It also affects practical outcomes such as mechanical harvesting efficiency and end-use quality. Despite its importance, the genetic basis of this trait remains unclear. To address this, we performed a genome-wide association study (GWAS) on a diverse panel, which identified a candidate locus containing Sobic.010G085400 (SbMADS52), a gene encoding a MADS-box transcription factor. Within this gene, we identified a putative functional G185S substitution associated with both stem thickness and plant height, suggesting a role in stalk architecture. To leverage this variant for breeding, we developed and validated a putative functional KASP marker. Population genetic analyses further revealed that the favorable allele has been under selection since domestication but is less frequent in modern breeding, highlighting an underused genetic resource. Collectively, our study characterizes a candidate regulator of stem morphology and provides a practical marker that may be used for sorghum breeding. CI - (c) 2026. The Author(s). FAU - Ma, Aimin AU - Ma A AD - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. FAU - Shi, Yingyi AU - Shi Y AD - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. FAU - Xu, Wenxiu AU - Xu W AD - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. FAU - Wu, Xiaoyuan AU - Wu X AD - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. FAU - Duan, Guoqi AU - Duan G AD - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. AD - University of Chinese Academy of Sciences, Beijing, 100049, China. FAU - Liu, Ruizhen AU - Liu R AD - College of Agriculture, Shanxi Agricultural University, Taigu, 030801, China. FAU - Yan, Huili AU - Yan H AD - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. AD - State Key Laboratory of Forage Breeding-by-Design and Utilization, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. FAU - Zhou, Yunzhuan AU - Zhou Y AD - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. FAU - Li, Jing AU - Li J AD - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. FAU - Li, Zhenfeng AU - Li Z AD - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. FAU - Li, Yutao AU - Li Y AD - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. FAU - Xuan, Wei AU - Xuan W AD - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. AD - State Key Laboratory of Forage Breeding-by-Design and Utilization, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. FAU - Jing, Haichun AU - Jing H AD - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. AD - State Key Laboratory of Forage Breeding-by-Design and Utilization, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. FAU - He, Zhenyan AU - He Z AD - Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. hezhenyan@ibcas.ac.cn. AD - State Key Laboratory of Forage Breeding-by-Design and Utilization, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China. hezhenyan@ibcas.ac.cn. LA - eng GR - 2023YFD1200700/National Key R&D Program of China/ GR - 2025-NK-126/Key Research and Development (R&D) and Transformation Program of Qinghai Province/ GR - 32573585/National Natural Science Foundation of China/ PT - Journal Article DEP - 20260801 PL - England TA - BMC Plant Biol JT - BMC plant biology JID - 100967807 RN - 0 (Genetic Markers) RN - 0 (Plant Proteins) SB - IM MH - *Sorghum/genetics/anatomy & histology/growth & development MH - *Domestication MH - *Plant Stems/genetics/anatomy & histology/growth & development MH - Alleles MH - Genetic Markers MH - Genome-Wide Association Study MH - Polymorphism, Single Nucleotide MH - *Plant Proteins/genetics MH - Plant Breeding MH - Genes, Plant MH - Phenotype OTO - NOTNLM OT - Sorghum bicolor OT - Domestication OT - KASP marker OT - Stem diameter COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:45 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/01 00:00 [received] PHST- 2026/07/30 00:00 [accepted] PHST- 2026/10/30 00:45 [entrez] AID - 10.1186/s12870-026-09674-5 [pii] AID - 10.1186/s12870-026-09674-5 [doi] PST - epublish SO - BMC Plant Biol. 2026 Aug 1;26(1):1846. doi: 10.1186/s12870-026-09674-5. PMID- 42958034 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2164 (Electronic) IS - 1471-2164 (Linking) VI - 27 IP - 1 DP - 2026 Aug 3 TI - Organellar genome analysis of Stephania epigaea: a medicinal plant endemic to Southwest China. LID - 899 [pii] LID - 10.1186/s12864-026-13240-6 [doi] AB - Mitochondria and chloroplast are central organelles in plant metabolism, playing crucial roles at various developmental stages. Stephania epigaea is a traditional medicinal plant utilized by minority ethnic groups, widely employed for treating malaria and stomach pain. Understanding the organelle genomes of S. epigaea-specifically its mitochondrial and chloroplast genomes-is essential for elucidating the molecular strategies underlying this species' evolutionary adaptation. The complete mitochondrial genome of this species is 476,231 bp in length and comprises three discrete circular chromosomes together with one additional complex structure. In contrast, the chloroplast genome is 158,139 bp and exhibits the canonical circular quadripartite architecture. A homologous segment of 9,723 bp was found to be shared with the chloroplast, involving the transfer of 24 homologous sequences. Relative Synonymous Codon Usage (RSCU) analysis indicates that the codon preference in the chloroplast genome of S. epigaea is generally higher than that in the mitochondria. Phylogenetic analysis indicates that S. epigaea shares a close genetic relationship with the congeneric plant S. japonica, and a substantial number of homologous blocks have been identified in the synteny analysis. Selection pressure analysis shows that the most of protein-coding genes (PCGs) exhibit a Ka/Ks ratio of less than 1, suggesting that these genes tend to maintain their original functions and avoid deleterious mutations. This study has significant implications for understanding the evolutionary relationships within the genus Stephania, laying a foundation for genetic research on the traditional medicine S. epigaea. CI - (c) 2026. The Author(s). FAU - Chen, Jian AU - Chen J AD - Yunnan Key Laboratory of Dai and Yi Medicines, Yunnan University of Chinese Medicine, Kunming, 650500, China. AD - College of Chinese Material Medica, Yunnan University of Chinese Medicine, Kunming, 650500, China. FAU - Yang, Congwei AU - Yang C AD - Yunnan Key Laboratory of Dai and Yi Medicines, Yunnan University of Chinese Medicine, Kunming, 650500, China. AD - College of Chinese Material Medica, Yunnan University of Chinese Medicine, Kunming, 650500, China. FAU - Zhang, Yingmin AU - Zhang Y AD - College of Chinese Material Medica, Yunnan University of Chinese Medicine, Kunming, 650500, China. FAU - Ma, Qianwen AU - Ma Q AD - College of Chinese Material Medica, Yunnan University of Chinese Medicine, Kunming, 650500, China. FAU - Wu, Lixin AU - Wu L AD - College of Chinese Material Medica, Yunnan University of Chinese Medicine, Kunming, 650500, China. wulixin@ynucm.edu.cn. FAU - Li, Guodong AU - Li G AD - Yunnan Key Laboratory of Dai and Yi Medicines, Yunnan University of Chinese Medicine, Kunming, 650500, China. gammar116@163.com. AD - College of Chinese Material Medica, Yunnan University of Chinese Medicine, Kunming, 650500, China. gammar116@163.com. LA - eng GR - 2024SS24088/the 12th Five-year Key Construction Discipline of State Administration of Traditional Chinese Medicine "Dai Pharmacy"/ GR - 2024JS2407/the 12th Five-year Key Construction Discipline of State Administration of Traditional Chinese Medicine "Dai Pharmacy"/ PT - Journal Article DEP - 20260803 PL - England TA - BMC Genomics JT - BMC genomics JID - 100965258 SB - IM MH - Phylogeny MH - *Genome, Chloroplast MH - *Plants, Medicinal/genetics MH - *Genome, Mitochondrial MH - China MH - Codon Usage MH - Evolution, Molecular MH - *Genomics/methods MH - *Genome, Plant OTO - NOTNLM OT - Stephania epigaea OT - Chloroplast OT - Mitogenome OT - Phylogenetic relationships OT - RNA-editing site OT - Repeat sequence COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:44 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/01/27 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/30 00:44 [entrez] AID - 10.1186/s12864-026-13240-6 [pii] AID - 10.1186/s12864-026-13240-6 [doi] PST - epublish SO - BMC Genomics. 2026 Aug 3;27(1):899. doi: 10.1186/s12864-026-13240-6. PMID- 42958033 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2164 (Electronic) IS - 1471-2164 (Linking) VI - 27 IP - 1 DP - 2026 Aug 1 TI - Ecotype-associated structural variants across multiple locally adapted Poeciliid fishes. LID - 898 [pii] LID - 10.1186/s12864-026-13205-9 [doi] AB - BACKGROUND: Extreme environments provide powerful natural laboratories for studying evolutionary convergence across taxa. While convergent phenotypes frequently evolve in response to similar selective pressures, consistent molecular signatures at the nucleotide level are often elusive, potentially due to overlooked genetic variants such as structural variants. Here, we leverage recent advances in long-read sequencing and pangenome approaches to investigate the role of structural variants in repeated adaptation to toxic springs rich in hydrogen sulfide (H(2)S) across multiple independently evolved populations within the Poecilia mexicana species complex. RESULTS: We identified more than 99,000 structural variants over 50 base pairs in length, including insertions, deletions, duplications and inversions, using complementary graph- and read-based methods. We then integrated these with population genomic data and RNA-sequencing data to detect variants in highly differentiated regions between ecotypes and assess their functional impacts. Structural variants in both coding and regulatory regions were enriched in genes involved in sulfide metabolism and ion transport, including ethe1, slc13a1, and sqor. We found ecotype-specific structural variants in promoter and intronic regions of key detoxification genes that exhibited elevated expression in H(2)S-adapted populations. CONCLUSIONS: Our findings suggest that structural variation may contribute to adaptive phenotypes and should be considered in studies of genomic convergence. CI - (c) 2026. The Author(s). FAU - Ryan, Kara AU - Ryan K AD - Department of Ecology and Evolutionary Biology, University of California Santa Cruz, Santa Cruz, CA, 95060, USA. FAU - De-Kayne, Rishi AU - De-Kayne R AD - Department of Ecology and Evolutionary Biology, University of California Santa Cruz, Santa Cruz, CA, 95060, USA. FAU - Arias-Rodriguez, Lenin AU - Arias-Rodriguez L AD - Division Academica de Ciencias Biologicas, Universidad Juarez Autonoma de Tabasco (UJAT), Villahermosa, Mexico. FAU - Tobler, Michael AU - Tobler M AD - Department of Biology, University of Missouri-St. Louis, St. Louis, MO, 63121, USA. AD - Whitney R. Harris World Ecology Center, University of Missouri-St. Louis, St. Louis, MO, 63121, USA. AD - WildCare Institute, Saint Louis Zoo, St. Louis, MO, 63110, USA. FAU - Kelley, Joanna L AU - Kelley JL AD - Department of Ecology and Evolutionary Biology, University of California Santa Cruz, Santa Cruz, CA, 95060, USA. jokelley@ucsc.edu. LA - eng GR - IOS-2311366 and IOS-2423844/National Science Foundation/ GR - 2311366/National Science Foundation/ PT - Journal Article DEP - 20260801 PL - England TA - BMC Genomics JT - BMC genomics JID - 100965258 RN - YY9FVM7NSN (Hydrogen Sulfide) SB - IM MH - Animals MH - *Poecilia/genetics/physiology MH - *Ecotype MH - *Adaptation, Physiological/genetics MH - Hydrogen Sulfide/metabolism MH - *Genetic Variation MH - Genomics OTO - NOTNLM OT - Adaptation OT - Convergent evolution OT - Extreme environments OT - Pangenome OT - Poecillidae OT - Structural variation COIS- Declarations. Ethics approval and consent to participate: Permits were provided by the Mexican government (DG0PA.090004.041111.3088, SGPA-DGVS-04315 and PRMN/DG0PA-003). The animal study protocol was reviewed and approved by the Institutional Animal Care and Use Committee of Kansas State University (IACUC protocol #3473). Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:44 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/06 00:00 [received] PHST- 2026/07/15 00:00 [accepted] PHST- 2026/10/30 00:44 [entrez] AID - 10.1186/s12864-026-13205-9 [pii] AID - 10.1186/s12864-026-13205-9 [doi] PST - epublish SO - BMC Genomics. 2026 Aug 1;27(1):898. doi: 10.1186/s12864-026-13205-9. PMID- 42958032 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2180 (Electronic) IS - 1471-2180 (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Ecological succession and topological rewiring of the oral microbiome in giant panda cubs. LID - 934 [pii] LID - 10.1186/s12866-026-05477-8 [doi] AB - In specialized herbivores retaining a carnivorous digestive tract, such as the giant panda, early-life oral microbiome assembly is crucial for lifelong health, yet remains poorly understood. Here, we longitudinally tracked the saliva and supragingival plaque microbiomes of captive cubs (180 matched samples) from birth to one year using microbiome sequencing and topological cartography. We demonstrate a profound spatial-temporal divergence driven by deterministic succession. Initial plaque colonization exhibited transient priority effects reflecting maternal imprints, rapidly superseded by autogenic niche reconstruction and deep anaerobic maturation. Host ontogeny, notably deciduous tooth eruption, triggered intense microbial exchange, causing significant transient habitat homogenization (peak Mantel correlations at months 6 and 8, p < 0.001). Mature plaque selectively enriched for peptidoglycan biosynthesis and complex carbohydrate degradation, establishing a pre-adaptive metabolic reservoir for the impending high-fiber bamboo diet. Crucially, topological analysis (interaction edges expanded 3.4-fold) revealed that early pioneers evade competitive exclusion by shifting their ecological niche to become inter-module connectors (Pi > 0.62), providing spatial scaffolds for late-arriving climax communities. These findings decipher the deterministic assembly rules of saliva and plaque microbiomes during the extreme milk-to-bamboo dietary transition, advancing our understanding of giant panda adaptive evolution and identifying native keystone targets for preventive veterinary interventions. CI - (c) 2026. The Author(s). FAU - Chen, Helin AU - Chen H AD - College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, 610000, Sichuan, China. AD - Key Laboratory of Animal Medicine of Sichuan Education Department, Southwest Minzu University, Chengdu, 610000, Sichuan, China. FAU - Liu, Jiabin AU - Liu J AD - The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, Chengdu Research Base of Giant Panda Breeding, Chengdu, 610000, Sichuan, China. FAU - Hou, Rong AU - Hou R AD - The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, Chengdu Research Base of Giant Panda Breeding, Chengdu, 610000, Sichuan, China. FAU - Chen, Fengli AU - Chen F AD - College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, 610000, Sichuan, China. AD - Key Laboratory of Animal Medicine of Sichuan Education Department, Southwest Minzu University, Chengdu, 610000, Sichuan, China. FAU - Qi, Dunwu AU - Qi D AD - The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, Chengdu Research Base of Giant Panda Breeding, Chengdu, 610000, Sichuan, China. FAU - Chen, Yu AU - Chen Y AD - College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, 610000, Sichuan, China. AD - Key Laboratory of Animal Medicine of Sichuan Education Department, Southwest Minzu University, Chengdu, 610000, Sichuan, China. FAU - Bi, Wenlei AU - Bi W AD - The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, Chengdu Research Base of Giant Panda Breeding, Chengdu, 610000, Sichuan, China. FAU - Zhou, Yanshan AU - Zhou Y AD - The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, Chengdu Research Base of Giant Panda Breeding, Chengdu, 610000, Sichuan, China. FAU - Gu, Jiang AU - Gu J AD - The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, Chengdu Research Base of Giant Panda Breeding, Chengdu, 610000, Sichuan, China. FAU - Lan, Guanwei AU - Lan G AD - The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, Chengdu Research Base of Giant Panda Breeding, Chengdu, 610000, Sichuan, China. FAU - Wu, Wei AU - Wu W AD - The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, Chengdu Research Base of Giant Panda Breeding, Chengdu, 610000, Sichuan, China. FAU - Zhang, Kai AU - Zhang K AD - College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu, 610000, Sichuan, China. AD - Key Laboratory of Animal Medicine of Sichuan Education Department, Southwest Minzu University, Chengdu, 610000, Sichuan, China. FAU - Ma, Rui AU - Ma R AUID- ORCID: 0009-0002-7253-3941 AD - The Conservation of Endangered Wildlife Key Laboratory of Sichuan Province, Chengdu Research Base of Giant Panda Breeding, Chengdu, 610000, Sichuan, China. marui_pandabase@outlook.com. LA - eng GR - ZYN2024057/Southwest Minzu University/ GR - 32400405/National Natural Science Foundation of China/ GR - 2024CPB-Y05/Chengdu Giant Panda Breeding Research Foundation/ PT - Journal Article DEP - 20260731 PL - England TA - BMC Microbiol JT - BMC microbiology JID - 100966981 RN - 0 (RNA, Ribosomal, 16S) SB - IM MH - Animals MH - *Ursidae/microbiology MH - *Microbiota MH - *Mouth/microbiology MH - *Bacteria/classification/genetics/isolation & purification MH - Saliva/microbiology MH - Dental Plaque/microbiology MH - Ecosystem MH - RNA, Ribosomal, 16S/genetics OTO - NOTNLM OT - Ailuropoda melanoleuca OT - Autogenic niche reconstruction OT - Dental plaque biofilm OT - Host ontogeny OT - Priority effects COIS- Declarations. Ethics approval and consent to participate: This study was conducted in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals. The study protocol, including the non-invasive oral specimen collection, was reviewed and approved by the Institutional Animal Care and Use Committee of the Chengdu Research Base of Giant Panda Breeding (Approval No. 201806). As the giant pandas involved in this study are captive animals bred and legally owned by the Chengdu Research Base of Giant Panda Breeding, written informed consent for their participation and for the collection of samples was obtained from the legal owner (the administrative authority of the Chengdu Research Base of Giant Panda Breeding). All sampling procedures complied with relevant institutional, national, and international guidelines for the care and use of animals. Because this study involved exclusively captive animals and non-invasive swabbing procedures within the institution's premises, no further specific field permits or local legislation licenses were required. Consent for publication: Not applicable. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:44 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/23 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/30 00:44 [entrez] AID - 10.1186/s12866-026-05477-8 [pii] AID - 10.1186/s12866-026-05477-8 [doi] PST - epublish SO - BMC Microbiol. 2026 Jul 31;26(1):934. doi: 10.1186/s12866-026-05477-8. PMID- 42958030 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2202 (Electronic) IS - 1471-2202 (Linking) VI - 27 IP - 1 DP - 2026 Aug 1 TI - The effects of urapidil as an alpha receptor blocker on spinal cord ischemia-reperfusion injury in male rats. LID - 51 [pii] LID - 10.1186/s12868-026-01032-3 [doi] AB - OBJECTIVES: The present experimental study was designed to evaluate the potential neuroprotective effects of urapidil during the acute phase (24 hours) of spinal cord ischemia-reperfusion injury in a rat model, with a focus on early functional, biochemical, histopathological, and immunohistochemical changes. METHODS: Rats were randomly assigned to four experimental groups (n = 6 per group): Control (CR), Sham (SH), Ischemia-Reperfusion (IR), and Ischemia-Reperfusion + Urapidil (IR+URA). Spinal cord ischemia was induced by infrarenal abdominal aortic clamping for 30 minutes followed by reperfusion. Urapidil was administered before ischemia and after reperfusion in the IR+URA group. Motor function was evaluated 24 hours after reperfusion using the Tarlov scoring system, and biochemical, histopathological, and immunohistochemical analyses were performed. Data were analyzed using one-way analysis of variance (ANOVA) followed by Duncan's multiple comparison test, which was selected due to its greater sensitivity in exploratory studies with small sample sizes. A p-value [Formula: see text] was considered statistically significant. RESULTS: Motor function was significantly impaired in the IR group compared with all other groups, whereas the IR+URA group demonstrated preserved motor performance comparable to the CR and SH groups. Urapidil administration significantly increased antioxidant activity, as reflected by elevated glutathione peroxidase levels, and reduced inflammatory markers including TNF-alpha and IL-1beta. Histopathological and immunohistochemical findings showed marked neuronal degeneration, apoptosis, oxidative stress, and inflammation in the IR group, which were notably attenuated by URA treatment. CONCLUSION: In this experimental spinal cord ischemia-reperfusion model, urapidil treatment was associated with improved early motor performance, increased glutathione peroxidase (GPx) activity, and reduced levels of the pro-inflammatory cytokines TNF-alpha and IL-1beta during the acute reperfusion period. These changes occurred alongside favorable histopathological findings; however, given the short follow-up duration and the limited scope of apoptotic assessment, the results should be considered preliminary and interpreted as hypothesis-generating. CI - (c) 2026. The Author(s). FAU - Keskin, Hasan Attila AU - Keskin HA AUID- ORCID: 0000-0001-6166-7237 AD - Cardiovascular Surgery Department, Health Ministry, Ankara Education and Training Hospital, Hacettepe Mah. Ulucanlar Cad. No:89, Altindag-Ankara, Turkey. attilakeskin@hotmail.com. FAU - Saritas, Zulfukar Kadir AU - Saritas ZK AD - Department of Surgery, Afyon Kocatepe University, Faculty of Veterinary Medicine, A.N.S Campus, Afyonkarahisar, 03200, Turkey. FAU - Ozbek, Fatma Gorucu AU - Ozbek FG AD - Department of Surgery, Afyon Kocatepe University, Faculty of Veterinary Medicine, A.N.S Campus, Afyonkarahisar, 03200, Turkey. FAU - Bulbul, Aziz AU - Bulbul A AD - Department of Physiology, Mugla Sitki Kocman University, Milas Veterinary Faculty, Mugla, Turkey. FAU - Koc, Yusuf AU - Koc Y AD - Department of Surgery, Afyon Kocatepe University, Faculty of Veterinary Medicine, A.N.S Campus, Afyonkarahisar, 03200, Turkey. FAU - Erdogan, Beyza Gul AU - Erdogan BG AD - Department of Surgery, Afyon Kocatepe University, Faculty of Veterinary Medicine, A.N.S Campus, Afyonkarahisar, 03200, Turkey. FAU - Danaci, Zeynep AU - Danaci Z AD - Department of Surgery, Afyon Kocatepe University, Faculty of Veterinary Medicine, A.N.S Campus, Afyonkarahisar, 03200, Turkey. FAU - Demireller, Basak AU - Demireller B AD - Department of Surgery, Afyon Kocatepe University, Faculty of Veterinary Medicine, A.N.S Campus, Afyonkarahisar, 03200, Turkey. FAU - Katircioglu, Salih Fehmi AU - Katircioglu SF AD - Cardiovascular Surgery Department, Health Ministry, Ankara Education and Training Hospital, Hacettepe Mah. Ulucanlar Cad. No:89, Altindag-Ankara, Turkey. LA - eng PT - Journal Article DEP - 20260801 PL - England TA - BMC Neurosci JT - BMC neuroscience JID - 100966986 RN - 0 (Neuroprotective Agents) RN - 0 (Piperazines) RN - A78GF17HJS (urapidil) RN - 0 (Tumor Necrosis Factor-alpha) RN - 0 (Interleukin-1beta) SB - IM MH - Animals MH - Male MH - *Reperfusion Injury/drug therapy/pathology/metabolism MH - *Spinal Cord Ischemia/drug therapy/pathology/metabolism MH - *Neuroprotective Agents/pharmacology MH - *Piperazines/pharmacology MH - Rats MH - Disease Models, Animal MH - Spinal Cord/drug effects/pathology/metabolism MH - Rats, Wistar MH - Oxidative Stress/drug effects MH - Tumor Necrosis Factor-alpha/metabolism MH - Apoptosis/drug effects MH - Interleukin-1beta/metabolism OTO - NOTNLM OT - Antioxidant OT - Neuroprotection OT - Reperfusion injury OT - Spinal cord ischemia OT - Urapidil COIS- Declarations. Ethics approval and consent to participate: This study was approved by the Afyon Kocatepe University Animal Experiments Local Ethics Committee (Approval No: 12/09/2023-49533702/80). All procedures were conducted in compliance with institutional guidelines and the ARRIVE guidelines. Consent to participate: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:44 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/11/03 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/30 00:44 [entrez] AID - 10.1186/s12868-026-01032-3 [pii] AID - 10.1186/s12868-026-01032-3 [doi] PST - epublish SO - BMC Neurosci. 2026 Aug 1;27(1):51. doi: 10.1186/s12868-026-01032-3. PMID- 42958022 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2229 (Electronic) IS - 1471-2229 (Linking) VI - 26 IP - 1 DP - 2026 Aug 3 TI - Distinct lipid and hormonal signatures reveal divergent responses to Erysiphe necator in resistant and susceptible grapevine genotypes. LID - 1854 [pii] LID - 10.1186/s12870-026-09654-9 [doi] AB - BACKGROUND: Powdery mildew, caused by the obligate biotroph Erysiphe necator, represents a major threat to grapevine production worldwide. Host-mediated resistance offers a sustainable alternative to chemical fungicides. To elucidate the role of plant membrane lipid modifications and oxylipin accumulation in pathogen perception and response, a controlled infection experiment was conducted comparing a resistant hybrid (NY_39) from the Edmund Mach Foundation germplasm collection with a susceptible variety (cv. 'Teroldego'). RESULTS: A lipidomic approach was integrated with hormone profiling and lipoxygenase (LOX) gene expression analysis. Significant lipid modulation was observed following E. necator inoculation, highlighting the plasticity of membrane and storage lipids metabolism. A rapid decrease in triacylglycerol content was measured in NY_39 at 12 h post-inoculation (hpi), whereas the opposite trend occurred in 'Teroldego', suggesting divergent metabolic rearrangements. A fast galactolipids peroxidation occurred in NY_39, while a delayed accumulation of phosphatidic acid was observed in 'Teroldego' at 48 hpi. The resistant genotype exhibited higher constitutive levels of salicylic acid as well as the expression of the VviAMP1 defensin, a small cysteine-rich protein with broad-spectrum antifungal activity. VviPR10-s11 and VviWRKY51, related to the synthesis of lignin and stilbenoid phytoalexins, were induced at 12 hpi in both genotypes. Within the LOX family, significant up-regulation of the 9-LOX VviLOX1a and the 13-LOXs VviLOX9 and VviLOXO was specific to 'Teroldego' at 12 hpi, despite a higher constitutive level of VviLOX9 in NY_39. CONCLUSIONS: This work identifies key metabolic, signalling and gene expression features in the selected hybrid that may be relevant for resistance. These include rapid triacylglycerols degradation, galactolipid peroxidation, constitutive higher SA level and expression of the defensin VviAMP1. CI - (c) 2026. The Author(s). FAU - Guche, Mikias D AU - Guche MD AD - C3A - Center Agriculture Food Environment, University of Trento, Via Edmund Mach 1, San Michele all'Adige, 38098, Italy. AD - Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, San Michele all'Adige, 38098, Italy. AD - Department of Sustainable Crop Production, Universita Cattolica del Sacro Cuore, Via Emilia Parmense 84, Piacenza, 29122, Italy. FAU - Dalla Costa, Lorenza AU - Dalla Costa L AD - Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, San Michele all'Adige, 38098, Italy. FAU - Lanubile, Alessandra AU - Lanubile A AD - Department of Sustainable Crop Production, Universita Cattolica del Sacro Cuore, Via Emilia Parmense 84, Piacenza, 29122, Italy. FAU - Mithofer, Axel AU - Mithofer A AD - Research Group Plant Defense Physiology, Max-Planck-Institute for Chemical Ecology, Hans-Knoll-Str. 8, Jena, D-07745, Germany. FAU - Masuero, Domenico AU - Masuero D AD - Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, San Michele all'Adige, 38098, Italy. FAU - Trenti, Francesco AU - Trenti F AD - Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, San Michele all'Adige, 38098, Italy. AD - Department of Physics, University of Trento, Via Sommarive 14, Povo, 38123, Italy. FAU - Garcia-Aloy, Mar AU - Garcia-Aloy M AD - Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, San Michele all'Adige, 38098, Italy. FAU - Vezzulli, Silvia AU - Vezzulli S AD - Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, San Michele all'Adige, 38098, Italy. FAU - Vrhovsek, Urska AU - Vrhovsek U AD - Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, San Michele all'Adige, 38098, Italy. FAU - Guella, Graziano AU - Guella G AD - Department of Physics, University of Trento, Via Sommarive 14, Povo, 38123, Italy. FAU - Malnoy, Mickael AU - Malnoy M AD - Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, San Michele all'Adige, 38098, Italy. FAU - Marocco, Adriano AU - Marocco A AD - Department of Sustainable Crop Production, Universita Cattolica del Sacro Cuore, Via Emilia Parmense 84, Piacenza, 29122, Italy. FAU - Pilati, Stefania AU - Pilati S AUID- ORCID: 0000-0001-7103-9613 AD - Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, San Michele all'Adige, 38098, Italy. stefania.pilati@fmach.it. LA - eng GR - PAT-ADP 2019-2021/Provincia Autonoma di Trento/ GR - PAT-ADP 2019-2021/Provincia Autonoma di Trento/ PT - Journal Article DEP - 20260803 PL - England TA - BMC Plant Biol JT - BMC plant biology JID - 100967807 RN - 0 (Plant Growth Regulators) RN - EC 1.13.11.12 (Lipoxygenase) RN - 0 (Plant Proteins) RN - O414PZ4LPZ (Salicylic Acid) SB - IM MH - *Vitis/genetics/microbiology/metabolism MH - *Plant Growth Regulators/metabolism MH - *Plant Diseases/microbiology/genetics/immunology MH - Genotype MH - *Erysiphe/physiology MH - *Disease Resistance/genetics MH - Lipoxygenase/metabolism/genetics MH - *Lipid Metabolism MH - Gene Expression Regulation, Plant MH - Plant Proteins/metabolism/genetics MH - Salicylic Acid/metabolism OTO - NOTNLM OT - Defensin OT - Lipidomic OT - Lipoxygenases OT - Powdery mildew OT - Salicylic acid OT - Vitis spp. COIS- Declarations. Ethics approval and consent to participate: Not applicable Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:44 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/24 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/30 00:44 [entrez] AID - 10.1186/s12870-026-09654-9 [pii] AID - 10.1186/s12870-026-09654-9 [doi] PST - epublish SO - BMC Plant Biol. 2026 Aug 3;26(1):1854. doi: 10.1186/s12870-026-09654-9. PMID- 42958017 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2164 (Electronic) IS - 1471-2164 (Linking) VI - 27 IP - 1 DP - 2026 Aug 4 TI - Whole genome comparative analysis shows limited Escherichia coli co-occurrence within co-habiting humans and dogs. LID - 900 [pii] LID - 10.1186/s12864-026-13236-2 [doi] AB - BACKGROUND: Dogs are one of the most popular companion animals in the UK, with an estimated 13.5 million within UK households. Within these households, human-animal physical interactions may lead to inter-species bacterial sharing, including Escherichia coli. E. coli is an opportunistic pathogen with a broad host range and can contribute to the spread of antimicrobial resistance (AMR). This work aimed to understand the extent of E. coli co-occurrence between hosts within the same household, and between dogs and humans. RESULTS: E. coli was non-selectively cultured from faecal samples obtained from 24 households with at least one human (n = 31) and one dog (n = 27) sample available at the same timepoint. Up to eight presumptive E. coli colonies were selected per sample and underwent short read whole genome sequencing. In total, 217 E. coli genomes were classified into 53 known sequence types (STs) and one unknown ST. Within samples, up to four different STs were present within each human and up to three different STs within a dog. Using a five single nucleotide polymorphism (SNP) threshold, there were 20 E. coli co-occurrences between hosts, including the same and different host species. Four instances involved potential cross-species co-occurrence within the same household. A genome-wide association study using a combined unitig and gene presence and absence approach identified one gene significantly more common in human derived samples; in silico characterisation revealed this gene is likely to have a function related to Type-F conjugative transfer system pilin assembly. Overall, 48.8% of the genomes had at least one genetic determinant associated with AMR, and 17.5% were classified as multidrug resistant. E. coli from humans carried more AMR determinants than E. coli from dogs. CONCLUSION: There was limited evidence of E. coli co-occurrence between different host species within households, and only one host-associated genetic feature. Humans harboured E. coli associated with more AMR determinants than those from dogs, suggesting that E. coli from humans presented a larger AMR source and AMR risk to other hosts in this dataset. Our findings can help inform risk analysis of E. coli sharing and transmission between humans and their companion dogs. CI - (c) 2026. The Author(s). FAU - Astorga, Gabriel AU - Astorga G AD - Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK. AD - Centre for Microbial Interactions, Norwich Research Park, Norwich, NR4 7UG, UK. FAU - Bloomfield, Samuel J AU - Bloomfield SJ AD - Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK. AD - Centre for Microbial Interactions, Norwich Research Park, Norwich, NR4 7UG, UK. FAU - Wain, John AU - Wain J AD - Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK. AD - Centre for Microbial Interactions, Norwich Research Park, Norwich, NR4 7UG, UK. FAU - de Oliveira Martins, Leonardo AU - de Oliveira Martins L AD - Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, L69 7ZF, UK. FAU - Singh, Dipali AU - Singh D AD - Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK. AD - Centre for Microbial Interactions, Norwich Research Park, Norwich, NR4 7UG, UK. FAU - Tiwari, Sumeet K AU - Tiwari SK AD - Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK. AD - Centre for Microbial Interactions, Norwich Research Park, Norwich, NR4 7UG, UK. FAU - Savva, George M AU - Savva GM AD - Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK. AD - Centre for Microbial Interactions, Norwich Research Park, Norwich, NR4 7UG, UK. FAU - Loeffler, Anette AU - Loeffler A AD - Department of Clinical Science and Services, Royal Veterinary College, Hertfordshire, Hatfield, AL9 7TA, UK. FAU - Menezes, Juliana AU - Menezes J AD - Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal. AD - Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Lisbon, Portugal. AD - I-MVET, Research in Veterinary Medicine, Faculty of Veterinary Medicine, Lusofona University - Lisbon University Centre, Lisbon, Portugal. FAU - Pomba, Constanca AU - Pomba C AD - Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal. AD - Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Lisbon, Portugal. FAU - Frosini, Sian Marie AU - Frosini SM AD - Department of Pathobiology and Population Sciences, Royal Veterinary College, Hertfordshire, Hatfield, AL9 7TA, UK. sfrosini@rvc.ac.uk. FAU - Mather, Alison E AU - Mather AE AD - Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK. Alison.Mather@quadram.ac.uk. AD - Centre for Microbial Interactions, Norwich Research Park, Norwich, NR4 7UG, UK. Alison.Mather@quadram.ac.uk. AD - University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK. Alison.Mather@quadram.ac.uk. LA - eng GR - [BB/T008717/1]/UKRI Biotechnology and Biological Sciences Research Council (BBSRC) Norwich Research Park Biosciences Doctoral Training Partnership/ GR - [BB/CCG1860/1]/Core Capability Grant/ GR - UIDB/00276/2020/Portuguese Foundation for Science and Technology (FCT) (CIISA)/ GR - [JPIAMR/0002/2016 ]/PET-Risk Consortium/ GR - [LA/P/0059/2020 ]/Portuguese Foundation for Science and Technology (FCT) (AL4AnimalS)/ GR - 2022.08669/PTDC (VetCare project)/ GR - DOI: 10.54499/2020.07562.BD/PhD fellowship/ GR - [MR/R000042/1]/Medical Research Council (MRC), UK/ GR - [BB/X011011/1]/BBSRC Institute Strategic Programme Microbes and Food Safety/ GR - [BBS/E/QU/230002A]/BBSRC Institute Strategic Programme Microbes and Food Safety (Theme 1, Microbial threats from foods in established and evolving food systems)/ PT - Comparative Study PT - Journal Article DEP - 20260804 PL - England TA - BMC Genomics JT - BMC genomics JID - 100965258 SB - IM MH - Animals MH - Dogs MH - *Escherichia coli/genetics/isolation & purification/classification MH - Humans MH - *Genome, Bacterial MH - Polymorphism, Single Nucleotide MH - *Escherichia coli Infections/microbiology/veterinary MH - Feces/microbiology MH - Whole Genome Sequencing OTO - NOTNLM OT - Escherichia coli OT - Antimicrobial Resistance OT - Companion Animals OT - Genome-Wide Association Study OT - Multidrug Resistance OT - One Health COIS- Declarations. Ethics approval and consent to participate: Faecal samples had been collected following signed, informed consent prior to enrolment as part of a previously described longitudinal observational study at the Royal Veterinary College (RVC), Hertfordshire, UK with ethical approval from the RVC's Clinical Research Ethical Review Board (CRERB; URN 2017 1750-3). Consent for publication: Informed consent was obtained from all individual participants included in the study and their companion animals. Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:43 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/09 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/30 00:43 [entrez] AID - 10.1186/s12864-026-13236-2 [pii] AID - 10.1186/s12864-026-13236-2 [doi] PST - epublish SO - BMC Genomics. 2026 Aug 4;27(1):900. doi: 10.1186/s12864-026-13236-2. PMID- 42958008 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1129-2377 (Electronic) IS - 1129-2369 (Linking) VI - 27 IP - 1 DP - 2026 Aug 1 TI - Along-tract white-matter microstructural differences in primary trigeminal neuralgia: a diffusion MRI tractometry study. LID - 253 [pii] LID - 10.1186/s10194-026-02482-0 [doi] AB - BACKGROUND: Primary trigeminal neuralgia (TN) is commonly linked to neurovascular compression (NVC), but NVC does not fully explain its clinical and imaging heterogeneity. Previous diffusion tensor imaging (DTI) studies have used tract-based spatial statistics or whole-tract averages, which may obscure localized within-tract differences. We used node-wise tractometry to map TN-related white-matter microstructural differences and test associations with NVC severity and pain laterality. METHODS: We studied 85 patients with primary TN and 42 healthy controls (HCs). Diffusion-weighted MRI at 3.0 T was preprocessed and fitted with a tensor model to derive fractional anisotropy (FA), mean diffusivity (MD), radial diffusivity (RD), and axial diffusivity (AD). Prespecified tracts were reconstructed with pyAFQ and resampled to 100 nodes. Among 23 prespecified bundles selected from 40 reconstructed bundles for statistical inference, primary analyses focused on the pyAFQ-defined Callosum Orbital bundle, anterior thalamic radiation, uncinate fasciculus, and cingulum. Node-wise linear models adjusted for age, sex, mean framewise displacement, and contrast-to-noise ratio were tested using Freedman-Lane permutations, threshold-free cluster enhancement (TFCE), and family-level false discovery rate (FDR) correction. RESULTS: Differences were most consistent in the Callosum Orbital bundle. Compared with HCs, patients with TN had lower FA (nodes 10-43 and 74-83; TFCE-corrected p = 0.009; FDR q = 0.038; peak beta = -0.031; 95% confidence interval [CI], -0.050 to -0.012), higher MD (p = 0.003; q = 0.011; peak beta = 0.035; 95% CI, 0.019 to 0.051), and higher RD (p < 0.001; q = 0.002; peak beta = 0.036; 95% CI, 0.019 to 0.052). MD and RD were also higher in the right uncinate fasciculus, and MD was higher in the right anterior thalamic radiation. No corrected differences were detected in the cingulum. Core tract metrics were not significantly associated after correction with Sindou grade, dichotomized NVC severity, or pain laterality. CONCLUSIONS: TN-related white-matter microstructural differences were segmental and tract selective, with the most consistent findings in the pyAFQ-defined Callosum Orbital bundle. These differences did not vary systematically with NVC severity or pain laterality, suggesting that they should not be interpreted simply as direct structural correlates of peripheral compression burden or unilateral pain input. CI - (c) 2026. The Author(s). FAU - Gao, Weiwei AU - Gao W AD - Department of Neurology, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China. FAU - Ma, Sicong AU - Ma S AD - Department of Neurology, The First Affiliated Hospital of Hunan University of Medicine, Huaihua, China. FAU - Huang, Rong AU - Huang R AD - Department of Neurology, Xiamen Humanity Hospital, Fujian Medical University, Xiamen, Fujian, China. FAU - She, Jingjing AU - She J AD - Department of Neurology, The First Affiliated Hospital of Hunan University of Medicine, Huaihua, China. FAU - Chen, Xingyu AU - Chen X AD - Department of Neurology, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China. chenxingyu200504@126.com. FAU - Li, Huaiyi AU - Li H AD - Department of Radiology, The Second Affiliated Hospital of Xiamen Medical College, Xiamen, China. huaiyili@yeah.net. FAU - Zhu, Renjing AU - Zhu R AD - Department of Neurology, Zhongshan Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, China. zhurenjing@yeah.net. LA - eng PT - Journal Article DEP - 20260801 PL - England TA - J Headache Pain JT - The journal of headache and pain JID - 100940562 SB - IM MH - Humans MH - *Trigeminal Neuralgia/diagnostic imaging/pathology MH - Female MH - *White Matter/diagnostic imaging MH - Diffusion Tensor Imaging/methods MH - Male MH - Middle Aged MH - Diffusion Magnetic Resonance Imaging MH - Aged MH - Adult OTO - NOTNLM OT - Callosum orbital bundle OT - Diffusion MRI OT - Diffusion tensor imaging OT - Neurovascular compression OT - Node-wise tractometry OT - Pain laterality OT - Primary trigeminal neuralgia OT - White matter microstructure COIS- Declarations. Ethics approval and consent to participate: The original study was approved by the institutional ethics committee of the Federal Neurosurgical Center in Novosibirsk, Russia. All participants provided written informed consent and agreed to public data sharing. The original study was conducted in accordance with the Declaration of Helsinki. This secondary analysis used a publicly available deidentified dataset and did not require additional ethics approval. Consent for publication: Not applicable; the manuscript contains no identifiable individual-level images, videos, or personal details. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:43 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/07/01 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/30 00:43 [entrez] AID - 10.1186/s10194-026-02482-0 [pii] AID - 10.1186/s10194-026-02482-0 [doi] PST - epublish SO - J Headache Pain. 2026 Aug 1;27(1):253. doi: 10.1186/s10194-026-02482-0. PMID- 42958004 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1471-2164 (Electronic) IS - 1471-2164 (Linking) VI - 27 IP - 1 DP - 2026 Aug 1 TI - Transcriptomic profiling and differential alternative splicing of citrus in response to infestation by Asian citrus psyllid, Diaphorina citri. LID - 894 [pii] LID - 10.1186/s12864-026-13241-5 [doi] AB - BACKGROUND: The Asian citrus psyllid, Diaphorina citri Kuwayama, is the principal vector of huanglongbing pathogens and poses a severe threat to citrus production worldwide. Transcriptional and post-transcriptional regulation mediated by alternative splicing (AS) are well-established mechanisms underlying plant resistance to insect infestation. Thus, a comprehensive understanding of these processes in citrus following D. citri infestation is essential for developing effective host resistance strategies. RESULTS: We investigated transcriptomic alterations and AS dynamics in citrus shoots at 24 h post-feeding (hpf24) and 48 h post-feeding (hpf48) following D. citri infestation. A total of 2,273 and 3,049 differentially expressed genes (DEGs) were identified at hpf24 and hpf48, respectively. Among these, 19 upregulated DEGs were associated with jasmonic acid (JA) biosynthesis and signaling pathways, indicating activation of JA-mediated defense responses. AS analysis revealed that feeding by D. citri significantly altered pre‑mRNA splicing. At hpf24, 422 differential alternative splicing (DAS) events corresponding to 363 genes were detected, and this number increased to 717 DAS events corresponding to 580 genes at hpf48, with intron retention being the predominant AS type. Integrative analysis of gene expression and AS profiles demonstrated a limited overlap between DEGs and differentially spliced genes. This suggests that AS may function, at least partially, independently of transcriptional regulation during citrus defense against D. citri infestation. Finally, nine JA-related genes were selected for validation of their relative expression levels by quantitative real-time PCR. CONCLUSIONS: Our study elucidates the coordinated transcriptomic and spliceomic reprogramming underlying citrus defense responses to D. citri infestation, highlighting the activation of JA‑mediated defenses and widespread AS events that largely operate independently of transcriptional changes. These findings provide novel molecular insights into citrus-psyllid interactions, with potential implications for future resistance breeding strategies. CI - (c) 2026. The Author(s). FAU - Zhu, Wenhong AU - Zhu W AD - College of Life Sciences, Gannan Normal University, Ganzhou, Jiangxi Province, China. FAU - Chen, Liang AU - Chen L AD - Jilin Academy of Agricultural Sciences, Changchun, Jilin Province, China. FAU - Shangguan, Chaozhi AU - Shangguan C AD - College of Life Sciences, Gannan Normal University, Ganzhou, Jiangxi Province, China. FAU - Zhong, Liqun AU - Zhong L AD - College of Life Sciences, Gannan Normal University, Ganzhou, Jiangxi Province, China. FAU - Yu, Xiudao AU - Yu X AD - College of Life Sciences, Gannan Normal University, Ganzhou, Jiangxi Province, China. yuxiudao@163.com. AD - Jiangxi Provincial Key Laboratory of Pest and Disease Control of Featured Horticultural Plants/National Navel Orange Engineering Research Center, Gannan Normal University, Ganzhou, Jiangxi Province, China. yuxiudao@163.com. LA - eng GR - 20250102311JC/Science and Technology Development Program of Jilin Province/ GR - 32560640/National Natural Science Foundation of China/ GR - 20212ACB215001/Science Fund for Distinguished Young Scholars of Jiangxi Province/ PT - Journal Article DEP - 20260801 PL - England TA - BMC Genomics JT - BMC genomics JID - 100965258 RN - 0 (Cyclopentanes) RN - 0 (Oxylipins) RN - 6RI5N05OWW (jasmonic acid) RN - Diaphorina citri SB - IM MH - Animals MH - *Alternative Splicing MH - *Hemiptera/physiology MH - *Gene Expression Profiling MH - *Citrus/genetics/parasitology MH - Cyclopentanes/metabolism MH - Oxylipins/metabolism MH - *Transcriptome MH - Gene Expression Regulation, Plant MH - Plant Diseases/parasitology/genetics OTO - NOTNLM OT - Citrus sinensis OT - Diaphorina citri OT - Intron retention OT - Jasmonic acid signaling OT - RNA-seq OT - Spliceosome reprogramming COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:43 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/18 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/30 00:43 [entrez] AID - 10.1186/s12864-026-13241-5 [pii] AID - 10.1186/s12864-026-13241-5 [doi] PST - epublish SO - BMC Genomics. 2026 Aug 1;27(1):894. doi: 10.1186/s12864-026-13241-5. PMID- 42958000 OWN - NLM STAT- Publisher LR - 20261030 IS - 2192-2659 (Electronic) IS - 2192-2640 (Linking) DP - 2026 Oct 29 TI - MXene-Integrated Composites for Next-Generation Photoelectrochemical Molecular Diagnostics. LID - e71907 [pii] LID - 10.1002/adhm.71907 [doi] AB - MXenes, a family of two-dimensional (2D) materials known for their characteristic electrical, optical, and surface properties, have emerged as promising candidates for advancing the performance of photoelectrochemical (PEC) biosensing technologies. When integrated into composites, including carbon-based materials, metal nanoparticles, and conductive polymers, MXenes' intrinsic properties, such as high surface area, high electrical conductivity, and tunable surface chemistry, can contribute to improving the sensitivity, selectivity, and stability of PEC biosensors. These characteristics make MXenes suitable for applications across various sectors, such as healthcare, food safety, and environmental monitoring. This review comprehensively outlines the core principles of PEC sensing, highlighting the role of MXene-based composites and light-driven processes in facilitating the detection of miRNA, protein biomarkers, small biomolecules, drug molecules, pesticides, and organic contaminants. It further explores recent advancements in MXene composites for detecting a broad spectrum of biological targets in complex biological samples. The review also addresses challenges, including the requirements for stability, reproducibility, biocompatibility, and scalability in various practical applications. Finally, it discusses future perspectives on MXene-integrated composites for PEC biosensing and their potential applications in clinical diagnostics, precision medicine, food safety, and environmental monitoring. CI - (c) 2026 Wiley-VCH GmbH. FAU - Natarajan, Bharathi AU - Natarajan B AUID- ORCID: 0009-0003-2625-1289 AD - College of Medicine, Jiaxing University, Jiaxing, Zhejiang, P. R. China. FAU - Kannan, Palanisamy AU - Kannan P AUID- ORCID: 0000-0002-1257-9692 AD - College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, Zhejiang, P. R. China. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - Germany TA - Adv Healthc Mater JT - Advanced healthcare materials JID - 101581613 SB - IM OTO - NOTNLM OT - MXene-integrated composites OT - healthcare applications OT - molecular diagnostics OT - photoelectrochemical biosensor EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:36 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/09/16 00:00 [revised] PHST- 2026/05/05 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/30 00:36 [entrez] AID - e71907 [pii] AID - 10.1002/adhm.71907 [doi] PST - aheadofprint SO - Adv Healthc Mater. 2026 Oct 29:e71907. doi: 10.1002/adhm.71907. PMID- 42957991 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261030 IS - 1861-471X (Electronic) IS - 1861-471X (Linking) VI - 21 IP - 21 DP - 2026 Nov TI - Copper-Catalyzed C horizontal line N Cross-Coupling in Water Enabled by Rational Ligand Design and Micellar Cooperativity. LID - e71031 [pii] LID - 10.1002/asia.71031 [doi] AB - Nitrogen-containing compounds are ubiquitous in pharmaceuticals, agrochemicals, and functional materials, and the development of sustainable methodologies for carbon-nitrogen (C horizontal line N) bond formation is of fundamental importance. Herein, we report a rationally designed bifunctional ligand and micellar cooperativity that effectively enables Cu-catalyzed amination of aryl bromides under mild aqueous conditions. A series of structurally tailored ligands was designed by systematically tuning their electronic and steric properties to stabilize the active copper catalyst and promote selective C horizontal line N bond formation. When combined with the bio-derived non-ionic surfactant CNSL-1000-M, the micellar environment provides a confined hydrophobic reaction space, enhancing catalyst performance and substrate activation. This cooperative ligand-micelle system enables the C horizontal line N coupling with both electron-rich and electron-deficient aryl bromides at 60 degrees C, affording the corresponding aryl amines in good to excellent yields. The active Cu catalyst was thoroughly characterized by UV-visible spectroscopy, x-ray photoelectron spectroscopy (XPS), and Electron Paramagnetic Resonance (EPR). It is worth noting that our current catalyst system shows better catalytic activity and selectivity than conventional organic solvents such as DMF, 1,4-dioxane, DMSO, and toluene. This study demonstrates that electronic, steric, and micellar cooperativity play decisive roles in aqueous copper-catalyzed reactions and establishes a practical, sustainable platform for C horizontal line N cross-coupling reactions. CI - (c) 2026 Wiley-VCH GmbH. FAU - Pradhan, Purusottam AU - Pradhan P AD - Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore, India. FAU - Yadav, Rajan AU - Yadav R AD - Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore, India. FAU - Kumari, Shalini AU - Kumari S AUID- ORCID: 0009-0001-9638-7849 AD - Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore, India. FAU - Hazra, Susanta AU - Hazra S AUID- ORCID: 0000-0002-4211-2970 AD - Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bangalore, India. LA - eng GR - EEQ/2022/000111/Anusandhan National Research Foundation/ GR - IE/CARE-22-0304/Indian Institute of Science/ PT - Journal Article PL - Germany TA - Chem Asian J JT - Chemistry, an Asian journal JID - 101294643 SB - IM EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:36 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/02 00:00 [revised] PHST- 2026/07/30 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/30 00:36 [entrez] AID - e71031 [pii] AID - 10.1002/asia.71031 [doi] PST - ppublish SO - Chem Asian J. 2026 Nov;21(21):e71031. doi: 10.1002/asia.71031. PMID- 42957982 OWN - NLM STAT- Publisher LR - 20261030 IS - 1525-1489 (Electronic) IS - 0885-0666 (Linking) DP - 2026 Oct 29 TI - Bacterial epidemiology and attributable mortality of Ventilator-associated pneumonia before and after COVID-19 pandemic (2018-2021). PG - 8850666261490896 LID - 10.1177/08850666261490896 [doi] AB - BackgroundVentilator-associated pneumonia (VAP) is a leading cause of morbidity and mortality in mechanically ventilated patients. The COVID-19 pandemic has profoundly modified VAP epidemiology and microbial ecology.MethodsWe conducted a prospective multicenter cohort study within the REA-REZO network ( approximately 20% of French ICU beds), 1 January 2018-31 December 2021. All adults receiving invasive mechanical ventilation were included. VAP was defined according to ECDC criteria. Primary endpoints were VAP incidence density (per 1000 ventilation-days) and 30-day attributable mortality. Secondary endpoints included resistance patterns and pre-pandemic versus pandemic trends.ResultsAmong 98,047 patients, 12,251 patients developed VAP, with an incidence of 18.9/1000 ventilation-days. Peak incidence occurred at day 10 (29/1000 ventilation-days). Thirty-day attributable mortality was 4.9% [95% CI 3.7;6.2], rising to 7.2% [6.0;8.5] at 60 days. Gram-negative bacteria accounted for 78.0% of isolates, predominantly Enterobacterales (46%) and Pseudomonas aeruginosa (20%). Third-generation cephalosporin resistance reached 27% in Enterobacterales and 25% in P. aeruginosa. The COVID-19 patients had a higher VAP rate (19.5% vs 9.7%, p<0.001) and mortality (36.9% vs 31.0%, p<0.001).ConclusionsVAP remains highly prevalent in French ICUs with substantial attributable mortality. The COVID-19 pandemic nearly doubled VAP incidence and worsened resistance profiles. Intensified prevention bundles during the first 10 days of ventilation and reinforced antimicrobial stewardship are critical. FAU - Vacheron, Charles Herve AU - Vacheron CH AUID- ORCID: 0000-0003-1575-5847 AD - Public Health, Epidemiology & Evolutionary Ecology of Infectious Diseases (PHE3ID) Team, Centre International de Recherche en Infectiologie (CIRI), Inserm U1111, CNRS UMR5308, ENS de Lyon, Universite Claude Bernard Lyon 1, Lyon, France. RINGGOLD: 55063 AD - Departement d'Anesthesie Reanimation, Hospices Civils de Lyon, Lyon, France. AD - REA-REZO Infections and Antimicrobial Resistance in Intensive Care, Lyon, France. FAU - Friggeri, Arnaud AU - Friggeri A AD - Public Health, Epidemiology & Evolutionary Ecology of Infectious Diseases (PHE3ID) Team, Centre International de Recherche en Infectiologie (CIRI), Inserm U1111, CNRS UMR5308, ENS de Lyon, Universite Claude Bernard Lyon 1, Lyon, France. RINGGOLD: 55063 AD - Departement d'Anesthesie Reanimation, Hospices Civils de Lyon, Lyon, France. AD - REA-REZO Infections and Antimicrobial Resistance in Intensive Care, Lyon, France. FAU - Machut, Anais AU - Machut A AD - REA-REZO Infections and Antimicrobial Resistance in Intensive Care, Lyon, France. AD - Prevention et Controle de l'Infection, Hospices Civils de Lyon, Lyon, France. FAU - Prevot, Claire AU - Prevot C AD - MSD France, Puteaux, France. RINGGOLD: 55447 FAU - Bourge, Xavier AU - Bourge X AD - MSD France, Puteaux, France. RINGGOLD: 55447 FAU - Savey, Anne AU - Savey A AD - REA-REZO Infections and Antimicrobial Resistance in Intensive Care, Lyon, France. AD - Prevention et Controle de l'Infection, Hospices Civils de Lyon, Lyon, France. FAU - Elias, Christelle AU - Elias C AD - REA-REZO Infections and Antimicrobial Resistance in Intensive Care, Lyon, France. AD - Service Hygiene, Epidemiologie, Infectiovigilance et Prevention, Hospices Civils de Lyon, Lyon, France. FAU - Lepape, Alain AU - Lepape A AD - REA-REZO Infections and Antimicrobial Resistance in Intensive Care, Lyon, France. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Intensive Care Med JT - Journal of intensive care medicine JID - 8610344 SB - IM OTO - NOTNLM OT - COVID-19 pandemic OT - antimicrobial resistance OT - attributable mortality OT - ventilator-associated pneumonia EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:35 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/30 00:35 [entrez] AID - 10.1177/08850666261490896 [doi] PST - aheadofprint SO - J Intensive Care Med. 2026 Oct 29:8850666261490896. doi: 10.1177/08850666261490896. PMID- 42957972 OWN - NLM STAT- Publisher LR - 20261030 IS - 1525-1489 (Electronic) IS - 0885-0666 (Linking) DP - 2026 Oct 29 TI - Impact of a Nurse-Led, Target-Directed Light Sedation Strategy on the Incidence of Delirium in Septic Patients in the Emergency Intensive Care Unit. PG - 8850666261496192 LID - 10.1177/08850666261496192 [doi] AB - ObjectiveTo evaluate the impact of a nurse-led, target-directed light sedation strategy on delirium incidence and short-term outcomes in septic patients undergoing invasive mechanical ventilation in the emergency intensive care unit (EICU).MethodsWe analyzed 221 consecutive septic patients requiring invasive mechanical ventilation for more than 24 h at a single center (January 2022-May 2024). Based on a sedation protocol change, patients were assigned to conventional sedation group (control, n = 123) or nurse-led, target-directed light sedation group (exposed, n = 98). The primary outcome was delirium incidence. Secondary outcomes included delirium-free days (DFD-28), ventilator-free days (VFD-28), mechanical ventilation duration, EICU length of stay, and 28-day mortality. Multivariable logistic regression examined the independent association between the nurse-led strategy and delirium risk.ResultsDelirium occurred less frequently in the nurse-led sedation group than in the control group (20.4% vs 37.4%, P = .007). The exposed group had shorter mechanical ventilation duration and EICU length of stay (both P < .05), whereas VFD-28 did not differ significantly between groups. The two groups did not differ significantly in 28-day mortality (28.6% vs 26.0%, P = .671) or in the rate of airway-related adverse events. On multivariable analysis, the nurse-led strategy was independently associated with a lower risk of delirium (OR = 0.428, 95% CI: 0.215-0.854, P = .016). Subgroup analyses showed that this protective benefit was consistent across age strata, disease severity levels, and comorbidity burden.ConclusionIn septic patients undergoing mechanical ventilation, a nurse-led, target-directed light sedation strategy was associated with reduced delirium incidence, shorter mechanical ventilation duration, and decreased EICU length of stay, without increasing adverse events. However, no improvement in 28-day mortality was observed. Further validation through multicenter prospective randomized controlled trials is warranted to assess long-term efficacy and safety. FAU - Wang, Lifen AU - Wang L AD - Department of Emergency Intensive Care Unit, The First Affiliated Hospital of Naval Medical University (Shanghai Changhai Hospital), Shanghai, P. R. China. FAU - Yuan, Zhuojun AU - Yuan Z AD - Department of Emergency Intensive Care Unit, The First Affiliated Hospital of Naval Medical University (Shanghai Changhai Hospital), Shanghai, P. R. China. FAU - Chen, Yuting AU - Chen Y AUID- ORCID: 0009-0001-4803-4782 AD - Department of Emergency Medicine, The Fourth People's Hospital, Tongji University, Shanghai, P. R. China. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Intensive Care Med JT - Journal of intensive care medicine JID - 8610344 SB - IM OTO - NOTNLM OT - delirium OT - emergency intensive care unit OT - light sedation OT - mechanical ventilation OT - nurse-led OT - sepsis EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:35 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/30 00:35 [entrez] AID - 10.1177/08850666261496192 [doi] PST - aheadofprint SO - J Intensive Care Med. 2026 Oct 29:8850666261496192. doi: 10.1177/08850666261496192. PMID- 42957971 OWN - NLM STAT- Publisher LR - 20261030 IS - 1525-1489 (Electronic) IS - 0885-0666 (Linking) DP - 2026 Oct 29 TI - A Decade Long Retrospective Cohort Study on Risk Factors and Clinical Outcomes of Carbapenem Resistant Gram-Negative Infections in A Pediatric Intensive Care Unit. PG - 8850666261500074 LID - 10.1177/08850666261500074 [doi] AB - BackgroundCarbapenem-resistant Gram-negative (CRGN) infections are a critical global health threat, particularly in pediatric intensive care units (PICUs). This study aimed to identify independent risk factors and clinical outcomes associated with CRGN infections in critically ill children.MethodsA 10-year (2015-2024) retrospective cohort study was conducted in a tertiary PICU, including 380 patients (aged 1 month-18 years) with Gram-negative infections. Patients were stratified into CRGN (n = 106) and carbapenem-susceptible (CSGN, n = 274) groups. Primary outcomes were independent risk factors for CRGN and 30-day all-cause mortality.ResultsThe overall CRGN prevalence was 27.9% (106/380), with an estimated absolute increase of 1.10 percentage points per year; however, the temporal trend was not statistically significant (p = .204). Predominant pathogens were Klebsiella spp. (31.1%) and E. coli (24.1%). Multivariate analysis identified polymicrobial infection (OR = 6.981, p < .001), mechanical ventilation (OR = 2.124, p = .025), systemic corticosteroid use (OR = 2.072, p = .012), underlying comorbidities (OR = 1.873, p = .022), and central venous catheters (OR = 1.906, p = .030) as independent risk factors for CRGN infection. The 30-day mortality was significantly higher in the CRGN group (27.4% vs 8.8%, p < .001). CRGN infection was the strongest independent predictor of mortality (OR = 2.49, p = .043), alongside PRISM III score and multiple comorbidities. The rate of appropriate empirical therapy was critically low in the CRGN group (36.8% vs 73.4%, p < .001).ConclusionsCRGN infections in the PICU are driven by invasive procedures and immunosuppression, associated with 2.48-fold higher odds of mortality. Early identification, tailored antimicrobial stewardship, and optimization of empirical therapy are urgently required to improve survival. FAU - Asik, Ayse AU - Asik A AUID- ORCID: 0000-0003-0973-8098 AD - Department of Pediatric Intensive Care, Goztepe Prof. Dr Suleyman Yalcin City Hospital, Istanbul, Turkiye. RINGGOLD: 652270 FAU - Duyu, Muhterem AU - Duyu M AUID- ORCID: 0000-0001-7892-2927 AD - Department of Pediatric Intensive Care, Goztepe Prof. Dr Suleyman Yalcin City Hospital, Istanbul, Turkiye. RINGGOLD: 652270 FAU - Yabanci Erten, Elif Selcen AU - Yabanci Erten ES AD - Department of Pediatrics, Goztepe Prof. Dr Suleyman Yalcin City Hospital, Istanbul, Turkiye. FAU - Dundar, Tansu AU - Dundar T AUID- ORCID: 0000-0003-3715-9579 AD - Department of Medical Microbiology, Goztepe Prof. Dr Suleyman Yalcin City Hospital, Istanbul, Turkiye. RINGGOLD: 652270 FAU - Pehlivan, Pelin AU - Pehlivan P AD - Department of Pediatric Intensive Care, Goztepe Prof. Dr Suleyman Yalcin City Hospital, Istanbul, Turkiye. RINGGOLD: 652270 FAU - Ozkavakli, Ayberk AU - Ozkavakli A AUID- ORCID: 0009-0002-0299-0725 AD - Department of Pediatrics, Faculty of Medicine, Bahcesehir University, Istanbul, Turkiye. RINGGOLD: 52946 LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Intensive Care Med JT - Journal of intensive care medicine JID - 8610344 SB - IM OTO - NOTNLM OT - antimicrobial stewardship OT - carbapenem resistance OT - gram-negative bacteria OT - mortality OT - pediatric intensive care unit OT - risk factors EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:35 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/30 00:35 [entrez] AID - 10.1177/08850666261500074 [doi] PST - aheadofprint SO - J Intensive Care Med. 2026 Oct 29:8850666261500074. doi: 10.1177/08850666261500074. PMID- 42957961 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 3085 DP - 2027 TI - Comprehensive Approach to Heterogenous Tumor Cell Dynamics Analysis for Intravital Microscopy. PG - 257-273 LID - 10.1007/978-1-0716-5591-7_16 [doi] AB - Intravital microscopy (IVM) enables observation of single-cell behaviors in live animal models, allowing detailed analysis of cancer cell dynamics within native 3D environments. However, comprehensively analyzing the extensive and complex data generated by IVM requires robust tools that exceed conventional approaches. This chapter presents a protocol for profiling heterogeneous cancer cell behavior within IVM movies, facilitating the study of cellular movement patterns and their associations with microenvironmental cues. The protocol allows researchers to systematically quantify single-cell (morpho)dynamics, providing unbiased analysis of cellular heterogeneity without requiring advanced computational expertise. The framework is adaptable to various tumor and non-tumor models, supporting insights into processes such as invasion, migration, and spatial relationships with distinct cell types, offering an accessible approach to studying heterogeneous cell behavior within its microenvironment. CI - (c) 2027. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Rios-Jimenez, Emilio AU - Rios-Jimenez E AD - Institute for Biomedical Research Sols-Morreale (IIBM), Spanish National Research Council-Universidad Autonoma de Madrid, Madrid, Spain. FAU - Archidona, Sandra F AU - Archidona SF AD - Institute for Biomedical Research Sols-Morreale (IIBM), Spanish National Research Council-Universidad Autonoma de Madrid, Madrid, Spain. FAU - Alieva, Maria AU - Alieva M AD - Institute for Biomedical Research Sols-Morreale (IIBM), Spanish National Research Council-Universidad Autonoma de Madrid, Madrid, Spain. malieva@iib.uam.es. LA - eng PT - Journal Article PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 SB - IM MH - *Intravital Microscopy/methods MH - Humans MH - Cell Movement MH - Animals MH - *Neoplasms/pathology MH - Tumor Microenvironment MH - Image Processing, Computer-Assisted/methods MH - Imaging, Three-Dimensional/methods MH - *Single-Cell Analysis/methods MH - Cell Line, Tumor OTO - NOTNLM OT - 3D image analysis OT - Cell dynamics OT - Cellular invasion OT - Motility patterns OT - Tumor heterogeneity OT - Unsupervised learning EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:34 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/30 00:34 [entrez] AID - 10.1007/978-1-0716-5591-7_16 [doi] PST - ppublish SO - Methods Mol Biol. 2027;3085:257-273. doi: 10.1007/978-1-0716-5591-7_16. PMID- 42957960 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 3085 DP - 2027 TI - Ex Vivo Models to Study the Interaction of Immune Cells and Cancer Cells Under Therapy. PG - 239-256 LID - 10.1007/978-1-0716-5591-7_15 [doi] AB - Recent developments in cancer therapeutics have focused on modulating the immune component of the tumor microenvironment in order to activate antitumoral effector functions and induce cytotoxic immune responses. To further advance the design of treatment strategies, it is essential to understand what factors within the tumor microenvironment limit therapeutic efficacy and immune responses. Intravital imaging is a pioneering technology that has been utilized to study these processes in an intact environment, successfully highlighting the importance of cancer cell-immune cell interactions such as cancer cell killing caused by longitudinal and repeated interactions with immune cells. Despite the clear advantages that intravital imaging provides, its low throughput limits the variety of drugs, genetically altered parameters, and cell-cell interactions that can be studied. In other fields, a complementary approach has been to turn to ex vivo models, which offer greater throughput and simpler experimental protocols. Here, we describe the setup of an ex vivo technique to study dynamic interactions within an intact environment. This chapter will, in detail, 1) demonstrate strategies to generate fluorescently tagged cancer and immune cells using genetic as well as dye-based approaches; 2) present a method for how to best generate lung metastasis for ex vivo imaging; 3) describe the protocol to generate precision cut lung metastasis slices; and 4) detail how to prepare them for multiphoton or confocal microscopy. This protocol can be utilized for following the effect of therapeutics on tumor growth within the lung microenvironment ex vivo and on the interaction of cancer cells with immune cells. Ex vivo lung slices are an excellent model to 1) prepare the design of follow-on intravital imaging experiments (e.g., determine appropriate fluorophores, drug concentrations, or cell types to label), 2) study immune and cancer cell interactions in a complete environment, 3) select promising drugs from a pool of targets, and 4) select the best timing for intravital imaging experiments. CI - (c) 2027. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Barth, Nicole D AU - Barth ND AD - Department of Pathology, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. nicole.barth@ed.ac.uk. AD - Cancer Research UK Edinburgh Centre, University of Edinburgh, Edinburgh, Scotland, UK. nicole.barth@ed.ac.uk. FAU - Oktay, Maja H AU - Oktay MH AD - Department of Pathology, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. AD - Cancer Dormancy Institute, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. AD - Department of Surgery, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. AD - Integrated Imaging Program for Cancer Research, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. AD - Montefiore Einstein Comprehensive Cancer Center, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. AD - Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. FAU - Condeelis, John S AU - Condeelis JS AD - Cancer Dormancy Institute, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. AD - Department of Surgery, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. AD - Integrated Imaging Program for Cancer Research, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. AD - Montefiore Einstein Comprehensive Cancer Center, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. AD - Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. AD - Department of Cell Biology, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. FAU - Brunton, Valerie G AU - Brunton VG AD - Cancer Research UK Edinburgh Centre, University of Edinburgh, Edinburgh, Scotland, UK. FAU - Entenberg, David AU - Entenberg D AD - Department of Pathology, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. david.entenberg@einsteinmed.edu. AD - Cancer Dormancy Institute, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. david.entenberg@einsteinmed.edu. AD - Integrated Imaging Program for Cancer Research, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. david.entenberg@einsteinmed.edu. AD - Montefiore Einstein Comprehensive Cancer Center, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. david.entenberg@einsteinmed.edu. AD - Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine/Montefiore Medical Center, Bronx, NY, USA. david.entenberg@einsteinmed.edu. LA - eng PT - Journal Article PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 SB - IM MH - Animals MH - Humans MH - Tumor Microenvironment/immunology MH - *Cell Communication/immunology MH - *Lung Neoplasms/immunology/secondary/pathology MH - Mice MH - *Neoplasms/immunology/pathology MH - Intravital Microscopy/methods MH - Cell Line, Tumor OTO - NOTNLM OT - Breast cancer OT - Cancer therapy response OT - Image analysis OT - Interaction of Immune cells and cancer cells OT - Metastasis OT - Precision cut lung slices OT - Two-photon microscopy EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:34 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/30 00:34 [entrez] AID - 10.1007/978-1-0716-5591-7_15 [doi] PST - ppublish SO - Methods Mol Biol. 2027;3085:239-256. doi: 10.1007/978-1-0716-5591-7_15. PMID- 42957951 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 3085 DP - 2027 TI - Intravital Multiphoton Microscopy of Schwann Cells in Mouse Models of Pancreatic Disease. PG - 95-107 LID - 10.1007/978-1-0716-5591-7_6 [doi] AB - Over the past decade, the important role of nerves in the growth and progression of pancreatic cancer has been increasingly recognized. The most abundant cell type present in the nerves, Schwann cells (SCs), can be reprogrammed by cancer cells and fibrotic extracellular matrix (ECM), facilitating perineural invasion of cancer cells in the pancreatic ductal adenocarcinoma (PDAC). Activated SCs form tracks, which are then populated and used by cancer cells for invasion. Intravital microscopy of the pancreas under the pathological conditions holds the potential to visualize and decipher real-time interactions of SCs with the cancer cells, ECM, and host cells in the tumor microenvironment and to delineate SC activation dynamics and the responses to targeted therapies. Here, we describe a protocol for high-resolution intravital microscopy of Schwann cells, ECM, and cancer cells using mouse models of PDAC or pancreatitis. CI - (c) 2027. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Stupakov, Pavel AU - Stupakov P AD - Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA. AD - Department of Surgery, TUM Universitatsklinikum Rechts der Isar, Technical University of Munich, School of Medicine, Munich, Germany. FAU - Velazquez-Quesada, Ines AU - Velazquez-Quesada I AD - Bioengineering Department, College of Engineering, Temple University, Philadelphia, PA, USA. FAU - Sadatrezaei, Golbahar AU - Sadatrezaei G AD - Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA. FAU - Gaino, Francesca AU - Gaino F AD - Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA. AD - Otorhinolaryngology-Head & Neck Surgery Unit, IRCCS Humanitas Research Hospital, Milan, Italy. AD - Department of Biomedical Sciences, Humanitas University, Milan, Italy. FAU - Wong, Richard J AU - Wong RJ AD - Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA. AD - David M. Rubenstein Center for Pancreatic Cancer Research, Memorial Sloan Kettering Cancer Center, New York, NY, USA. FAU - Deborde, Sylvie AU - Deborde S AD - Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA. debordes@mskcc.org. AD - David M. Rubenstein Center for Pancreatic Cancer Research, Memorial Sloan Kettering Cancer Center, New York, NY, USA. debordes@mskcc.org. FAU - Gligorijevic, Bojana AU - Gligorijevic B AD - Bioengineering Department, College of Engineering, Temple University, Philadelphia, PA, USA. bojana@temple.edu. AD - Cancer Signaling and Microenvironment Program, Fox Chase Cancer Center, Philadelphia, PA, USA. bojana@temple.edu. AD - Department of Cancer and Cellular Biology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA, USA. bojana@temple.edu. LA - eng PT - Journal Article PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 SB - IM MH - Animals MH - *Schwann Cells/pathology/metabolism MH - *Intravital Microscopy/methods MH - Mice MH - *Pancreatic Neoplasms/pathology MH - Disease Models, Animal MH - *Carcinoma, Pancreatic Ductal/pathology MH - Tumor Microenvironment MH - Humans MH - *Pancreatitis/pathology MH - Extracellular Matrix/metabolism/pathology MH - *Microscopy, Fluorescence, Multiphoton/methods OTO - NOTNLM OT - Collagen fibers OT - Intravital microscopy OT - Pancreas OT - Pancreatic cancer OT - Pancreatitis OT - Schwann cells OT - Tumor microenvironment EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:34 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/30 00:34 [entrez] AID - 10.1007/978-1-0716-5591-7_6 [doi] PST - ppublish SO - Methods Mol Biol. 2027;3085:95-107. doi: 10.1007/978-1-0716-5591-7_6. PMID- 42957950 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 3085 DP - 2027 TI - Intravital Imaging of the In Situ-to-Invasive Transition of Breast Cancer. PG - 77-94 LID - 10.1007/978-1-0716-5591-7_5 [doi] AB - Ductal carcinoma in situ (DCIS) is the precursor of infiltrating ductal carcinoma (IDC); however, mechanisms underlying the invasive program of breast tumor epithelial cells remain largely unknown. Different assays and cell models have been developed to evaluate the invasive capacities of breast tumor cells (spheroids, organoids, tumor explants...). These models recapitulate basic interactions between the neoplastic cells and their microenvironment in a simplified setup. Whenever possible, hypotheses and mechanisms should be validated based on the observation of tumor cells in their native environment in the mammary gland using animal models. Here, we describe specific tools to study early breast tumor progression, taking into consideration reducing the number of xenografted animals and having the capacity to follow tumor progression over time. Combinations of these approaches have revealed new and exciting insights as to explain how DCIS progresses into a more aggressive invasive lesion. This chapter details technical procedures to generating in situ tumors after intraductal inoculation, visualizing the tumor xenograft directly in the mammary gland of the injected mouse using a mammary window for intravital multi-photon imaging of tumor cells in their environment. Finally, we illustrate how this procedure can be useful to analyze morphological changes in the matrix microenvironment during early breast cancer progression. CI - (c) 2027. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Irondelle, Marie AU - Irondelle M AD - Centre Mediterraneen de Medecine Moleculaire, Universite Cote d'Azur, INSERM, Nice, France. FAU - Chavrier, Philippe AU - Chavrier P AD - Institut Curie, CNRS UMR 144, PSL Research University, Paris, France. FAU - Lodillinsky, Catalina AU - Lodillinsky C AD - Instituto de Oncologia Angel H. Roffo, Area deInvestigacion Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina. clodillinsky@cbc.uba.ar. AD - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires, Argentina. clodillinsky@cbc.uba.ar. LA - eng PT - Journal Article PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 SB - IM MH - Animals MH - Female MH - Humans MH - *Breast Neoplasms/pathology/diagnostic imaging MH - *Intravital Microscopy/methods MH - Mice MH - *Carcinoma, Intraductal, Noninfiltrating/pathology/diagnostic imaging MH - Neoplasm Invasiveness MH - Tumor Microenvironment MH - Cell Line, Tumor MH - Disease Progression OTO - NOTNLM OT - Breast cancer OT - Ductal carcinoma in situ (DCIS) OT - Infiltrating ductal carcinoma (IDC) OT - Intraductal injection OT - Intravital imaging EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:34 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/30 00:34 [entrez] AID - 10.1007/978-1-0716-5591-7_5 [doi] PST - ppublish SO - Methods Mol Biol. 2027;3085:77-94. doi: 10.1007/978-1-0716-5591-7_5. PMID- 42957947 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 3085 DP - 2027 TI - Fluorescent Probes for Intravital Imaging of Cellular Apoptosis. PG - 17-32 LID - 10.1007/978-1-0716-5591-7_2 [doi] AB - Apoptosis or programmed cell death is a process that is dysregulated in many diseases, including inflammatory conditions and cancer. It is therefore crucial to detect apoptosis for the screening of anticancer therapeutics and in mechanistic studies using preclinical models; however, conventional reagents for imaging cell death (e.g., fluorescent annexins) are limited by the need of washing steps, incompatibility with physiological conditions or poor biodistribution. We have reported fluorogenic ApoTracker probes as alternative tools for selective fluorescence labeling of apoptotic cells in a calcium-independent manner and under wash-free conditions. In this Chapter, we describe protocols using ApoTracker probes for the detection and quantification of apoptosis in cells from different origins by flow cytometry and live-cell imaging. We have optimized methods that are compatible with fixed tissue samples and with no interference with the clearance of apoptotic cells by phagocytes. We provide procedures to assess the effect of chemotherapeutic drugs in screenings with cancer cells and in preclinical mouse models of inflammation and cancer by intravital imaging. CI - (c) 2027. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Mendive-Tapia, Lorena AU - Mendive-Tapia L AD - Centre for Inflammation Research, Institute for Regeneration and Repair, The University of Edinburgh, EH16 4UU, Edinburgh, UK. lorena.mendive@gmail.com. AD - IRR Chemistry Hub, Institute for Regeneration and Repair, The University of Edinburgh, EH16 4UU, Edinburgh, UK. lorena.mendive@gmail.com. FAU - Karmakar, Utsa AU - Karmakar U AD - Centre for Inflammation Research, Institute for Regeneration and Repair, The University of Edinburgh, EH16 4UU, Edinburgh, UK. AD - IRR Chemistry Hub, Institute for Regeneration and Repair, The University of Edinburgh, EH16 4UU, Edinburgh, UK. FAU - Vendrell, Marc AU - Vendrell M AD - Centre for Inflammation Research, Institute for Regeneration and Repair, The University of Edinburgh, EH16 4UU, Edinburgh, UK. marc.vendrell@ed.ac.uk. AD - IRR Chemistry Hub, Institute for Regeneration and Repair, The University of Edinburgh, EH16 4UU, Edinburgh, UK. marc.vendrell@ed.ac.uk. LA - eng PT - Journal Article PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 RN - 0 (Fluorescent Dyes) RN - 0 (Fluorescent Chemosensor Compounds) SB - IM MH - *Apoptosis/drug effects MH - Animals MH - *Fluorescent Dyes/chemistry MH - Humans MH - Mice MH - *Intravital Microscopy/methods MH - Flow Cytometry/methods MH - Cell Line, Tumor MH - Female MH - Fluorescent Chemosensor Compounds OTO - NOTNLM OT - Acute lung injury OT - Breast cancer OT - Chemotherapy OT - Drug screening OT - Fluorogenic probes OT - Neutrophils OT - Peptides EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:34 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/30 00:34 [entrez] AID - 10.1007/978-1-0716-5591-7_2 [doi] PST - ppublish SO - Methods Mol Biol. 2027;3085:17-32. doi: 10.1007/978-1-0716-5591-7_2. PMID- 42957946 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 3085 DP - 2027 TI - Third Harmonic Generation Microscopy for Label-Free Imaging of Cells, Tissues, and the Tumor Microenvironment. PG - 1-15 LID - 10.1007/978-1-0716-5591-7_1 [doi] AB - Third harmonic generation (THG) microscopy is a label-free nonlinear imaging technique that visualizes refractive index boundaries within biological specimens, generating contrast at plasma membranes, lipid interfaces, vesicles, and extracellular matrix structures. Because THG does not require fluorescent labeling and induces minimal phototoxicity, it enables high-resolution imaging of living cells, tissues, and tumors in their native state. THG is particularly powerful in cancer research, where it reveals tumor cell morphology, vesicle-rich leading edges, stromal organization, adipocyte remodeling, vascular interfaces, and immune cell dynamics in vivo. This chapter provides practical guidance for implementing THG microscopy, including optical requirements, filter configurations for combined THG/SHG/fluorescence detection, sample preparation, and acquisition strategies. Detailed protocols are provided for (i) forward-detected THG imaging of tumor cells embedded in three-dimensional collagen matrices and (ii) backward-detected THG imaging of ex vivo skin and tumors growing in the dermis in vivo. Together, these workflows enable comprehensive, label-free visualization of cellular and microenvironmental features relevant to cancer invasion and tissue architecture. CI - (c) 2027. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Giampetraglia, Martina AU - Giampetraglia M AD - Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University of Tubingen, Tubingen, Germany. AD - Cluster of Excellence iFIT (EXC 2180) "Image-Guided and Functionally Instructed Tumor Therapies", University of Tubingen, Tubingen, Germany. FAU - Quach, Julia AU - Quach J AD - Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University of Tubingen, Tubingen, Germany. AD - Cluster of Excellence iFIT (EXC 2180) "Image-Guided and Functionally Instructed Tumor Therapies", University of Tubingen, Tubingen, Germany. FAU - Weigelin, Bettina AU - Weigelin B AD - Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University of Tubingen, Tubingen, Germany. bettina.weigelin@med.uni-tuebingen.de. AD - Cluster of Excellence iFIT (EXC 2180) "Image-Guided and Functionally Instructed Tumor Therapies", University of Tubingen, Tubingen, Germany. bettina.weigelin@med.uni-tuebingen.de. LA - eng PT - Journal Article PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 SB - IM MH - *Tumor Microenvironment MH - Animals MH - Humans MH - Mice MH - *Second Harmonic Generation Microscopy/methods MH - Cell Line, Tumor OTO - NOTNLM OT - Cancer invasion OT - Extracellular matrix OT - Intravital microscopy OT - Label-free imaging OT - Multiphoton microscopy OT - Nonlinear optics OT - Skin imaging OT - THG microscopy OT - Third harmonic generation OT - Tumor microenvironment EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:34 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/30 00:34 [entrez] AID - 10.1007/978-1-0716-5591-7_1 [doi] PST - ppublish SO - Methods Mol Biol. 2027;3085:1-15. doi: 10.1007/978-1-0716-5591-7_1. PMID- 42957938 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 1869-4101 (Print) IS - 1869-4101 (Linking) VI - 17 IP - 1 DP - 2026 Oct 29 TI - MRI-based proton density fat fraction combined with adipose tissue for identifying metabolic dysfunction-associated steatohepatitis. LID - 268 [pii] LID - 10.1186/s13244-026-02444-9 [doi] AB - OBJECTIVES: To develop a non-invasive diagnostic model combining magnetic resonance imaging (MRI)-derived proton density fat fraction (PDFF) and adipose tissue for identifying metabolic dysfunction-associated steatohepatitis (MASH). MATERIALS AND METHODS: A total of 197 consecutive patients with metabolic dysfunction-associated steatotic liver disease (MASLD) who underwent liver biopsy, MRI-PDFF, and transient elastography (TE) were retrospectively enrolled. Independent predictors of MASH were identified using univariate and multivariate logistic regression analyses. A combined model was constructed and internally validated via 1000 bootstrap resamples. The diagnostic performance was compared with that of established non-invasive tests (NITs). RESULTS: Multivariable logistic regression analyses demonstrated that the PDFF and total adipose tissue index (TATI) were independent risk factors for MASH. The MRI-based PDFF-adipose tissue (MR-PAT) model achieved an area under the receiver operating characteristic curve (AUC) of 0.866 for MASH identification. Bootstrap internal validation yielded an AUC of 0.870. The performance of the models was consistent across clinically relevant subgroups. The diagnostic performance of the MR-PAT model for MASH identification was significantly better than that of all the established NITs (all p < 0.05). CONCLUSION: The MR-PAT model, combining MRI-derived PDFF and TATI, provides an accurate and robust non-invasive tool for identifying MASH. It outperforms widely recognized NITs and holds promise for risk stratification in patients with MASLD. KEY POINTS: Question MASH is a critical histological endpoint of MASLD, but reliable non-invasive tools for its assessment remain inadequate. Findings The MR-PAT model integrating MRI-based PDFF and adipose tissue quantification demonstrates robust and excellent diagnostic performance for identifying MASH. Critical Relevance Statement The MR-PAT model leverages hepatic steatosis and the adipose tissue burden to elucidate the pathophysiological mechanisms driving steatohepatitis. It can serve as an accurate, non-invasive tool for identifying MASH and holds promise for risk stratification in patients with MASLD. CI - (c) 2026. The Author(s). FAU - Yang, Yiming AU - Yang Y AD - Department of Radiology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, Guangdong, China. AD - The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, 510120, Guangdong, China. FAU - Lai, Simei AU - Lai S AD - The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, 510120, Guangdong, China. FAU - Li, Xiaohuan AU - Li X AD - The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, 510120, Guangdong, China. FAU - Zhou, Dongjing AU - Zhou D AD - Department of Radiology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, Guangdong, China. FAU - Chen, Zhiyuan AU - Chen Z AD - Department of Radiology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, Guangdong, China. FAU - Ma, Zelan AU - Ma Z AD - Department of Radiology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, Guangdong, China. FAU - Zhang, Xiaoting AU - Zhang X AD - Department of Radiology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, Guangdong, China. FAU - Liu, Yupin AU - Liu Y AUID- ORCID: 0000-0001-6287-0755 AD - Department of Radiology, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, Guangdong, China. mdliuyupin@163.com. LA - eng GR - 20254054/Scientific Research Project of Traditional Chinese Medicine Bureau of Guangdong Provincial/ GR - YX2026QN001/Program for Chronic Liver Disease Imaging Research of Guangdong Hospital of Chinese Medicine/ GR - YX2026MS002/Program for Chronic Liver Disease Imaging Research of Guangdong Hospital of Chinese Medicine/ PT - Journal Article DEP - 20261029 PL - Germany TA - Insights Imaging JT - Insights into imaging JID - 101532453 OTO - NOTNLM OT - Adipose tissue OT - Magnetic resonance imaging OT - Metabolic dysfunction-associated steatohepatitis OT - Proton density fat fraction COIS- Declarations. Ethics approval and consent to participate: The authors are accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013). Approval for the study was obtained from the Ethics Committee of the Second Affiliated Hospital of Guangzhou University of Chinese Medicine (BE2023-204-01). Consent for publication: Not applicable. Competing interests: All authors declare that they have no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:34 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/22 00:00 [received] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/08/09 00:00 [revised] PHST- 2026/10/30 00:34 [entrez] AID - 10.1186/s13244-026-02444-9 [pii] AID - 10.1186/s13244-026-02444-9 [doi] PST - epublish SO - Insights Imaging. 2026 Oct 29;17(1):268. doi: 10.1186/s13244-026-02444-9. PMID- 42957933 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 3005-074X (Electronic) IS - 3005-074X (Linking) VI - 10 IP - 1 DP - 2026 Oct 30 TI - Restoration of radioiodine avidity after lenvatinib in NRAS- and TERT promoter-mutant radioiodine-refractory differentiated thyroid cancer: a case report. LID - 56 [pii] LID - 10.1186/s41824-026-00334-3 [doi] AB - To our knowledge, clinically meaningful restoration of radioiodine avidity in established radioiodine-refractory differentiated thyroid cancer has not been reported after multikinase inhibitor therapy, despite successful redifferentiation reported with selective kinase inhibitors targeting BRAF, RAS, RET or NTRK. We report a 53-year-old woman with invasive follicular variant papillary thyroid carcinoma and synchronous cervical lymph node, pulmonary and bone metastases who underwent six cycles of radioiodine therapy within 3 years after total thyroidectomy. Initially radioiodine-avid metastases gradually lost radioiodine avidity, as suggested by serial post-radioiodine therapy whole-body scans. Lenvatinib was initiated after 3 years of active surveillance. Restored radioiodine uptake was observed on rhTSH-assisted diagnostic whole-body scan after 9 months of lenvatinib treatment, allowing subsequent salvage radioiodine therapy. Subsequent post-radioiodine therapy whole-body scans showed increased uptake intensity and additional radioiodine-avid bone metastases, with a marked decrease in suppressed thyroglobulin. Reassessment of radioiodine avidity after prolonged multikinase inhibitor treatment warrants clinical consideration to determine the feasibility of salvage radioiodine therapy. CI - (c) 2026. The Author(s). FAU - Chen, Kexu AU - Chen K AD - Department of Nuclear Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College (PUMC) Hospital, Chinese Academy of Medical Sciences & PUMC, No.1 Shuaifuyuan, Wangfujing, Dongcheng District, Beijing, 100730, China. AD - Department of Nuclear Medicine, Qingdao Central Hospital, University of Health and Rehabilitation Sciences (Qingdao Central Hospital), Qingdao, Shandong, China. FAU - Zhao, Yihan AU - Zhao Y AD - Department of Nuclear Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College (PUMC) Hospital, Chinese Academy of Medical Sciences & PUMC, No.1 Shuaifuyuan, Wangfujing, Dongcheng District, Beijing, 100730, China. FAU - Shi, Cong AU - Shi C AD - Department of Nuclear Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College (PUMC) Hospital, Chinese Academy of Medical Sciences & PUMC, No.1 Shuaifuyuan, Wangfujing, Dongcheng District, Beijing, 100730, China. FAU - Li, Zheng AU - Li Z AD - Department of Nuclear Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College (PUMC) Hospital, Chinese Academy of Medical Sciences & PUMC, No.1 Shuaifuyuan, Wangfujing, Dongcheng District, Beijing, 100730, China. AD - Department of Nuclear Medicine Center, Beijing Nuclear Industry Hospital, Beijing, China. FAU - Lin, Yansong AU - Lin Y AUID- ORCID: 0000-0001-6095-4728 AD - Department of Nuclear Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College (PUMC) Hospital, Chinese Academy of Medical Sciences & PUMC, No.1 Shuaifuyuan, Wangfujing, Dongcheng District, Beijing, 100730, China. linys@pumch.cn. LA - eng GR - No. 82472027/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261030 PL - England TA - EJNMMI Rep JT - EJNMMI reports JID - 9918752082606676 OTO - NOTNLM OT - Bone metastasis OT - Diagnostic whole-body scan OT - Lenvatinib OT - Multikinase inhibitor OT - Radioiodine avidity OT - Radioiodine-refractory thyroid cancer OT - Redifferentiation COIS- Declarations. Ethics approval and consent to participate: This case report was conducted in accordance with the Declaration of Helsinki and was approved by the Ethics Committee of Peking Union Medical College Hospital (Approval No. I-24PJ2359). Consent for publication: Written informed consent for publication of this case and any accompanying images was obtained from the patient. Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:33 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/07/09 00:00 [received] PHST- 2026/08/26 00:00 [accepted] PHST- 2026/10/30 00:33 [entrez] AID - 10.1186/s41824-026-00334-3 [pii] AID - 10.1186/s41824-026-00334-3 [doi] PST - epublish SO - EJNMMI Rep. 2026 Oct 30;10(1):56. doi: 10.1186/s41824-026-00334-3. PMID- 42957921 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 2199-1170 (Print) IS - 2198-9761 (Linking) VI - 12 IP - 1 DP - 2026 Oct 29 TI - Acute and Chronic Effects of Parachute-Resisted Sprint Training on Sprint Performance and Sprint Mechanics: A Systematic Review with GRADE Assessment. LID - 161 [pii] LID - 10.1186/s40798-026-01131-9 [doi] AB - BACKGROUND: Parachute-resisted sprint training (PRST) is a practical field-based resisted-sprint method that uses aerodynamic drag to provide sprint-specific overload. Although PRST has been widely used in athletic settings, evidence on its acute mechanical effects and chronic training adaptations remains heterogeneous. OBJECTIVES: This systematic review aimed to synthesize the acute and chronic effects of PRST on sprint performance, sprint mechanics, neuromuscular responses, and sprint-related physiological outcomes in healthy, physically active, and athletic populations. METHODS: A systematic search was conducted in PubMed, Scopus, Web of Science, and SPORTDiscus on 17th May 2026. Original empirical studies were eligible if they examined PRST or parachute-resisted sprinting and reported outcomes related to sprint performance, biomechanics, neuromuscular responses, or sprint-related physiology. Studies were synthesized narratively and grouped into acute and chronic evidence streams. Methodological quality was assessed using the Modified Downs and Black checklist, and certainty of evidence was rated using the GRADE approach. RESULTS: Seventeen studies were included (8 acute and 9 chronic). In acute comparisons, PRST consistently reduced sprint velocity or increased sprint time; where directly reported, velocity reductions were approximately 3.4-6% relative to unresisted sprinting. Larger parachute conditions generally produced greater velocity decrements and changes in horizontal force or impulse variables. Chronic studies reported improvements in selected sprint outcomes, including 0-10 m, 10-20 m, and 0-20 m splits in one randomized study; other studies reported improvements such as 3.48% in 60-yd performance and 0.618 s in 100-m performance. However, chronic studies' findings were heterogeneous, and evidence for maximal velocity, neuromuscular or energetic outcomes, and population-specific effects remained limited. CONCLUSIONS: PRST appears to provide a practical, sprint-specific overload and may improve selected sprint performance outcomes. However, the current evidence base is limited by small sample sizes, heterogeneous protocols, poor quantification of resistance, inconsistent reporting of outcomes, and limited comparator control. Based on GRADE, confidence in the evidence is generally 'low' to 'very low' across most outcomes. Trial Registration (OSF) https://osf.io/56nct DOI: 10.17605/OSF.IO/8SJW4. CI - (c) 2026. The Author(s). FAU - Bruno, Joao AU - Bruno J AUID- ORCID: 0000-0001-9656-1563 AD - CIPER, Faculty of Sport Sciences and Physical Education, University of Coimbra, Coimbra, Portugal. AD - Portuguese Air Force, Avenida da Forca Aerea Portuguesa, Amadora, Portugal. FAU - Thapa, Rohit Kumar AU - Thapa RK AUID- ORCID: 0000-0002-1258-9065 AD - Symbiosis School of Sports Sciences, Symbiosis International (Deemed University), Pune, India. rohit.thapa@ssss.edu.in. FAU - Sarmento, Hugo AU - Sarmento H AUID- ORCID: 0000-0001-8681-0642 AD - CIPER, Faculty of Sport Sciences and Physical Education, University of Coimbra, Coimbra, Portugal. LA - eng PT - Journal Article PT - Systematic Review DEP - 20261029 PL - Switzerland TA - Sports Med Open JT - Sports medicine - open JID - 101662568 OTO - NOTNLM OT - Acceleration OT - Biomechanics OT - Certainty of evidence OT - Methodological quality OT - Narrative synthesis COIS- Declarations. Ethics Approval and Consent to Participate: Not applicable. This study is a systematic review of previously published studies and did not involve the recruitment of human participants, collection of new human data, or use of individual-level identifiable information. Consent for Publication: Not applicable. Competing Interests: The authors declare that they have no financial or non-financial competing interests, including professional interests, personal relationships, or other circumstances that could reasonably be perceived as influencing the work reported in this manuscript. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:32 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/06/08 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/30 00:32 [entrez] AID - 10.1186/s40798-026-01131-9 [pii] AID - 10.1186/s40798-026-01131-9 [doi] PST - epublish SO - Sports Med Open. 2026 Oct 29;12(1):161. doi: 10.1186/s40798-026-01131-9. PMID- 42957919 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1941-6636 (Electronic) VI - 57 IP - 1 DP - 2026 Oct 30 TI - Survival Outcomes in Metastatic Colorectal Cancer Patients with BRAF Mutations Undergoing Targeted Chemotherapy: A Reconstructed Individual Patient Data Meta-Analysis of Randomized Controlled Trials. LID - 249 [pii] LID - 10.1007/s12029-026-01597-6 [doi] AB - BACKGROUND: BRAF V600E-mutant metastatic colorectal cancer (mCRC) represents an aggressive molecular subtype associated with poor prognosis and limited response to conventional chemotherapy. Although multiple targeted strategies, including anti-vascular endothelial growth factor (VEGF), anti-epidermal growth factor receptor (EGFR), and BRAF-directed therapies, are used in clinical practice, their comparative effectiveness remains uncertain. We performed a reconstructed individual patient-data (IPD) meta-analysis of randomized controlled trials (RCTs) to evaluate survival outcomes across targeted therapeutic classes in BRAF-mutant mCRC. METHODS: A systematic search of PubMed, Embase, Scopus, and Cochrane databases was conducted through July 2025 to identify RCTs reporting survival outcomes for BRAF-mutant mCRC. Kaplan-Meier curves were digitized and IPD reconstructed using established methods. A one-stage meta-analysis using Cox proportional hazards models was performed to estimate pooled overall survival (OS) and progression-free survival (PFS). Models were adjusted for study-level covariates and clustering, with Bonferroni correction applied for multiple comparisons. RESULTS: Eighteen RCTs comprising 11,931 participants were included. Pooled median OS was 22.3 months for BRAF-directed regimens, 17.1 months for VEGF-targeted therapy, and 10.1 months for EGFR-directed therapy across studies evaluating first-line therapy. Across first-line studies, BRAF inhibitor directed regimen was associated with significantly improved OS compared with EGFR inhibition (HR 0.43, 95% CI 0.31-0.60; P < 0.001) and improved PFS (HR 0.47, 95% CI 0.42-0.53; P < 0.001). VEGF inhibition also demonstrated superior OS compared with EGFR inhibition (HR 0.56, 95% CI 0.38-0.81; p < 0.001). EGFR-directed regimen consistently demonstrated the poorest survival outcomes across analyses. DISCUSSION: In this reconstructed IPD meta-analysis, BRAF-directed therapies were associated with the most favourable survival outcomes, whereas EGFR directed regimen demonstrated limited clinical benefit in BRAF-mutant mCRC. These results highlight the importance of biologically aligned pathway-targeted therapy and provide comparative evidence to inform treatment selection in this aggressive molecular subtype. CI - (c) 2026. The Author(s). FAU - Kazmi, Syeda Kanza AU - Kazmi SK AUID- ORCID: 0000-0001-6386-6221 AD - Department of Internal Medicine, Penn State Health Milton S. Hershey Medical Center, Hershey, United States. skazmi1@pennstatehealth.psu.edu. FAU - Rehman, Muhammad Abdul AU - Rehman MA AUID- ORCID: 0000-0003-3540-0998 AD - Mayo Clinic School of Graduate Medical Education, Mayo Clinic, Rochester, MN, United States. AD - Division of Gastroenterology & Hepatology, Mayo Clinic, Rochester, MN, United States. AD - Department of Internal Medicine, University of Central Florida, Tallahassee, FL, USA. FAU - Tariq, Hanaa AU - Tariq H AUID- ORCID: 0009-0004-8375-7304 AD - Sindh Medical College, Jinnah Sindh Medical University, Karachi, Pakistan. FAU - Kabir, Aqsa AU - Kabir A AD - Dow Medical College, Dow University of Health Sciences, Karachi, Pakistan. FAU - Ahmed, Jalib AU - Ahmed J AD - Dow Medical College, Dow University of Health Sciences, Karachi, Pakistan. FAU - Yee, Nelson Shu-Sang S AU - Yee NSS AD - Division of Hematology & Oncology, Department of Internal Medicine, Penn State Health Milton S. Hershey Medical Center, Hershey, United States. LA - eng PT - Journal Article PT - Meta-Analysis DEP - 20261030 PL - United States TA - J Gastrointest Cancer JT - Journal of gastrointestinal cancer JID - 101479627 RN - EC 2.7.11.1 (Proto-Oncogene Proteins B-raf) RN - EC 2.7.11.1 (BRAF protein, human) RN - EC 2.7.10.1 (ErbB Receptors) SB - IM MH - Humans MH - *Proto-Oncogene Proteins B-raf/genetics/antagonists & inhibitors MH - *Colorectal Neoplasms/drug therapy/genetics/mortality/pathology MH - Randomized Controlled Trials as Topic MH - Mutation MH - *Molecular Targeted Therapy/methods MH - ErbB Receptors/antagonists & inhibitors MH - *Antineoplastic Combined Chemotherapy Protocols/therapeutic use OTO - NOTNLM OT - Anti-EGFR OT - BRAF V600E mutation OT - Metastatic colorectal cancer OT - Targeted Therapy OT - anti-VEGF OT - meta-analysis COIS- Declarations. Financial: None to declare. Competing interests: Dr. Nelson Shu-Sang S. Yee has received support for attending meetings and/or travel from Total Health Oncology. Disclosures: Parts of this manuscript were presented as abstracts at the 2025 American College of Gastroenterology (ACG) in Phoenix, Arizona; and separately at Digestive Disease Week 2026 in Chicago, Illinois, USA. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:32 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/13 00:00 [received] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/30 00:32 [entrez] AID - 10.1007/s12029-026-01597-6 [pii] AID - 10.1007/s12029-026-01597-6 [doi] PST - epublish SO - J Gastrointest Cancer. 2026 Oct 30;57(1):249. doi: 10.1007/s12029-026-01597-6. PMID- 42957918 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1559-1182 (Electronic) IS - 0893-7648 (Linking) VI - 63 IP - 1 DP - 2026 Oct 30 TI - Exercise and Mitophagy in Cerebral Ischemia: Mechanistic Evidence, Exercise Strategies, and Translational Challenges. LID - 1001 [pii] LID - 10.1007/s12035-026-06284-y [doi] AB - Following ischemic stroke, energy depletion, mitochondrial depolarization, oxidative stress, and disrupted calcium homeostasis lead to the accumulation of damaged mitochondria, which in turn amplifies neuroinflammation and cell death. As a selective mechanism for eliminating damaged mitochondria, mitophagy contributes to mitochondrial quality control and may represent one of the mechanisms involved in exercise-mediated neuroprotection after ischemic stroke. This review synthesizes evidence on mitophagy in cerebral ischemia and its regulation by exercise. We discuss the ubiquitin-dependent PINK1/Parkin pathway, the receptor-dependent BNIP3L/NIX and FUNDC1 pathways, and their upstream regulation by AMPK/ULK1, SIRT3, Sesn2/AMPK/TFEB, and PGC-1alpha/FNDC5/irisin signaling. We also compare exercise preconditioning with post-stroke training in terms of initiation time, modality, intensity, and dose. Preclinical evidence indicates that exercise improves neurobehavioral outcomes after cerebral ischemia and enhances mitochondrial respiration, dynamics, and biogenesis. However, direct evidence that exercise protects ischemic brain tissue through mitophagy remains limited. Most studies rely on static markers, including LC3, p62, PINK1, Parkin, and BNIP3L/NIX, which cannot distinguish mitophagy-specific flux or establish causality. The optimal timing, intensity, and dose of exercise also remain undefined. Future studies should integrate mitochondria-targeted flux reporters, genetic interventions, and standardized reporting of exercise parameters using the frequency, intensity, time, type, volume, and progression (FITT-VP) framework to clarify the mechanistic contribution of mitophagy and inform future translational research. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Wang, Xinzheng AU - Wang X AD - Faculty of Health Sciences and Physical Education, Macao Polytechnic University, Macao, China. 20171028@zcmu.edu.cn. AD - Department of Physical Education, Zhejiang Chinese Medical University, Hangzhou, 310053, Zhejiang Province, China. 20171028@zcmu.edu.cn. FAU - Luo, Hongbin AU - Luo H AD - Department of Physical Education, Zhejiang Chinese Medical University, Hangzhou, 310053, Zhejiang Province, China. FAU - Yi, Guohao AU - Yi G AD - Department of Physical Education, Zhejiang Chinese Medical University, Hangzhou, 310053, Zhejiang Province, China. FAU - Zhou, Huifen AU - Zhou H AD - School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, 310053, Zhejiang Province, China. FAU - Wang, Bihan AU - Wang B AD - School of Physical Education, Hunan Normal University, Changsha, 410012, China. FAU - Tu, Qiang AU - Tu Q AD - School of Health Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia. LA - eng PT - Journal Article PT - Review DEP - 20261030 PL - United States TA - Mol Neurobiol JT - Molecular neurobiology JID - 8900963 SB - IM MH - *Mitophagy/physiology MH - Animals MH - Humans MH - *Brain Ischemia/pathology/metabolism/physiopathology MH - Mitochondria/metabolism MH - *Exercise/physiology MH - *Translational Research, Biomedical MH - Signal Transduction OTO - NOTNLM OT - Exercise OT - Ischemic stroke OT - Mitochondrial quality control OT - Mitophagy OT - PINK1/Parkin COIS- Declarations. Ethics Approval: Not applicable. Informed Consent: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:32 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/08/17 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/30 00:32 [entrez] AID - 10.1007/s12035-026-06284-y [pii] AID - 10.1007/s12035-026-06284-y [doi] PST - epublish SO - Mol Neurobiol. 2026 Oct 30;63(1):1001. doi: 10.1007/s12035-026-06284-y. PMID- 42957910 OWN - NLM STAT- Publisher LR - 20261030 IS - 1460-2075 (Electronic) IS - 0261-4189 (Linking) DP - 2026 Oct 28 TI - Modeling long-range human nigrostriatal connectivity in vitro reveals striatal target-dependent maturation of dopamine neurons. LID - 10.1038/s44318-026-00942-6 [doi] AB - The human nigrostriatal pathway, comprising dopaminergic neurons in the ventral midbrain that project to the dorsolateral striatum, is essential for motor control and selectively vulnerable in Parkinson's disease. How this circuit assembles during human development remains poorly understood, and in vitro models capturing its long-range connectivity and spatial organization have been lacking. Here, we establish a compartmentalized, human stem cell-based model of the nigrostriatal pathway by integrating ventral midbrain and striatal organoids within a custom-engineered microfluidic device that confines cell bodies while guiding axonal growth to recapitulate key aspects of in vivo topography. Functional connectivity was confirmed by retrograde rabies tracing, and optogenetic and pharmacological stimulation. Single-nucleus transcriptomic analysis reveals that axonal target engagement with spatially separated striatal neurons promotes maturation of human dopaminergic neurons and activates transcriptional programs associated with neurotransmitter secretion. Our study establishes a biologically relevant in vitro system for investigating how long-range connectivity regulates neuronal assembly in the nigrostriatal circuit and for modelling key pathological features of Parkinson's disease. CI - (c) 2026. The Author(s). FAU - Corsi, Sara AU - Corsi S AUID- ORCID: 0000-0002-2584-6446 AD - Developmental and Regenerative Neurobiology, Lund Stem Cell Center, Department of Experimental Medical Science, Faculty of Medicine, Lund University, Lund, Sweden. sara.corsi.8878@med.lu.se. FAU - Sozzi, Edoardo AU - Sozzi E AUID- ORCID: 0000-0002-6303-1969 AD - Developmental and Regenerative Neurobiology, Lund Stem Cell Center, Department of Experimental Medical Science, Faculty of Medicine, Lund University, Lund, Sweden. AD - Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland. FAU - Bruzelius, Andreas AU - Bruzelius A AD - Developmental and Regenerative Neurobiology, Lund Stem Cell Center, Department of Experimental Medical Science, Faculty of Medicine, Lund University, Lund, Sweden. FAU - Scordo, Giorgio AU - Scordo G AUID- ORCID: 0000-0003-0559-9668 AD - Department of Biotechnology and Biomedicine (DTU Bioengineering), Technical University of Denmark, Kongens Lyngby, Denmark. FAU - Ramos Passarello, German AU - Ramos Passarello G AD - Developmental and Regenerative Neurobiology, Lund Stem Cell Center, Department of Experimental Medical Science, Faculty of Medicine, Lund University, Lund, Sweden. FAU - Zannino, Clara AU - Zannino C AUID- ORCID: 0000-0003-1919-3212 AD - Developmental and Regenerative Neurobiology, Lund Stem Cell Center, Department of Experimental Medical Science, Faculty of Medicine, Lund University, Lund, Sweden. FAU - Tavares da Silva Maraschin, Samuel AU - Tavares da Silva Maraschin S AD - Department of Biotechnology and Biomedicine (DTU Bioengineering), Technical University of Denmark, Kongens Lyngby, Denmark. FAU - Thongkorn, Surangrat AU - Thongkorn S AUID- ORCID: 0000-0003-4149-9381 AD - Department of Biotechnology and Biomedicine (DTU Bioengineering), Technical University of Denmark, Kongens Lyngby, Denmark. FAU - Heiskanen, Arto AU - Heiskanen A AD - Department of Biotechnology and Biomedicine (DTU Bioengineering), Technical University of Denmark, Kongens Lyngby, Denmark. FAU - Kajtez, Janko AU - Kajtez J AUID- ORCID: 0000-0001-9997-2325 AD - Developmental and Regenerative Neurobiology, Lund Stem Cell Center, Department of Experimental Medical Science, Faculty of Medicine, Lund University, Lund, Sweden. AD - Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. FAU - Emneus, Jenny AU - Emneus J AUID- ORCID: 0000-0002-8061-201X AD - Department of Biotechnology and Biomedicine (DTU Bioengineering), Technical University of Denmark, Kongens Lyngby, Denmark. AD - Department of Chemistry, Center for Analysis and Synthesis, Faculty of Engineering & Faculty of Science, Lund University, Lund, Sweden. FAU - Parmar, Malin AU - Parmar M AUID- ORCID: 0000-0001-5002-4199 AD - Developmental and Regenerative Neurobiology, Lund Stem Cell Center, Department of Experimental Medical Science, Faculty of Medicine, Lund University, Lund, Sweden. malin.parmar@med.lu.se. LA - eng GR - 101047177/EC | Horizon Europe | Innovative Europe | HORIZON EUROPE European Innovation Council (EIC)/ GR - 101167102/EC | Horizon Europe | Excellent Science | HORIZON EUROPE European Research Council (ERC)/ GR - 2021-00661/Vetenskapsradet (VR)/ GR - 2021-02967/Vetenskapsradet (VR)/ GR - 1675/25/Swedish Parkinson's Disease Association | Parkinsonfonden (Parkinson Foundation)/ GR - FO2024-01/Hjarnfonden (Brain Foundation)/ GR - FO2026/222/Konung Gustaf V:s och Drottning Victorias Frimurarestiftelse (King Gustaf V and Queen Victoria's Foundation of Freemasons)/ GR - 45540/Kungliga Fysiografiska Sallskapet i Lund (Royal Physiographic Society in Lund)/ GR - F2024/1518/Mats Paulssons Stiftelse (Mats Paulsson Foundation)/ PT - Journal Article DEP - 20261028 PL - England TA - EMBO J JT - The EMBO journal JID - 8208664 SB - IM COIS- Disclosure and competing interests statement. MP is the owner of Parmar Cells AB, co-inventor of the following patents WO2016162747A2, WO2018206798A1, and WO2019016113A1, performs paid Consultancy and commissioned research for Novo Nordisk AS Cell Therapy Research and Development unit, and serves on the SAB for Arbor Bio. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:32 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/12/19 00:00 [received] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/30 00:32 [entrez] AID - 10.1038/s44318-026-00942-6 [pii] AID - 10.1038/s44318-026-00942-6 [doi] PST - aheadofprint SO - EMBO J. 2026 Oct 28. doi: 10.1038/s44318-026-00942-6. PMID- 42957905 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1617-4623 (Electronic) IS - 1617-4623 (Linking) VI - 301 IP - 1 DP - 2026 Oct 30 TI - High LIFR expression is associated with poor prognosis of stomach adenocarcinoma. LID - 209 [pii] LID - 10.1007/s00438-026-02541-w [doi] AB - Gastric cancer remains a leading cause of cancer-related death worldwide, yet its pathogenesis remains incompletely understood. Leukemia inhibitory factor receptor (LIFR) has been implicated in the initiation and progression of gastric cancer by regulating the proliferation, invasion and metastasis of gastric cancer cells. In this study, we used The Cancer Genome Atlas (TCGA) and other public databases to investigate the expression and prognostic value of LIFR in stomach adenocarcinoma (STAD) through bioinformatics approaches. We found that LIFR expression was significantly lower in tumor tissues than in paracancerous tissues. Notably, within tumor tissues, low LIFR expression was associated with a more favorable prognosis. We further divided STAD patients into high- and low-LIFR-expression groups and analyzed the differentially expressed genes, constructed an upstream ceRNA regulatory network, and examined immune infiltration and DNA methylation levels. Finally, the bioinformatics findings were validated by functional cell experiments, the results of which were largely consistent with the database analyses. Taken together, the biological role of LIFR in STAD is context-dependent and cannot be simply classified as tumor-promoting or tumor-suppressive. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Tian, Yihao AU - Tian Y AD - Department of Pathology, General Hospital of Northern Theater Command, Beifang Hospital of China Medical University, No.83, Wenhua Road, Shenhe District, Shenyang, 110004, Liaoning, China. FAU - Liu, Hongjing AU - Liu H AD - Department of Hepatobiliary Pancreatic Splenic and Thyroid Surgery, General Hospital of Northern Theater Command, No.83, Wenhua Road, Shenhe District, Shenyang, 110004, Liaoning, China. 1357729081@qq.com. LA - eng PT - Journal Article DEP - 20261030 PL - Germany TA - Mol Genet Genomics JT - Molecular genetics and genomics : MGG JID - 101093320 RN - 0 (LIFR protein, human) RN - 0 (Leukemia Inhibitory Factor Receptor alpha Subunit) RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - *Stomach Neoplasms/genetics/pathology/metabolism MH - Prognosis MH - *Adenocarcinoma/genetics/pathology/metabolism MH - Gene Expression Regulation, Neoplastic MH - *Leukemia Inhibitory Factor Receptor alpha Subunit/genetics/metabolism MH - DNA Methylation/genetics MH - *Biomarkers, Tumor/genetics MH - Gene Regulatory Networks MH - Cell Line, Tumor OTO - NOTNLM OT - Biomarker OT - LIFR OT - Prognosis OT - STAD OT - TCGA COIS- Declarations. Conflict of interest: The authors declare no competing interests. Ethical approval and consent to participate: Not applicable. Consent for publication: Not applicable. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:31 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/07/19 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/30 00:31 [entrez] AID - 10.1007/s00438-026-02541-w [pii] AID - 10.1007/s00438-026-02541-w [doi] PST - epublish SO - Mol Genet Genomics. 2026 Oct 30;301(1):209. doi: 10.1007/s00438-026-02541-w. PMID- 42957903 OWN - NLM STAT- Publisher LR - 20261030 IS - 1759-4782 (Electronic) IS - 1759-4774 (Linking) DP - 2026 Oct 28 TI - Combining locoregional treatments and immunotherapies in patients with unresectable hepatocellular carcinoma. LID - 10.1038/s41571-026-01210-6 [doi] AB - The systemic treatment landscape of unresectable hepatocellular carcinoma has been transformed by immune-checkpoint inhibitor (ICI)-based regimens. These advances have led to efforts to combine ICIs with locoregional therapies (LRTs), particularly transarterial chemoembolization, transarterial radioembolization and external beam radiotherapy, in an attempt to improve tumour control and extend the benefits of ICIs to patients with intermediate-stage disease. The rationale for this combination approach is provided by the ability of LRTs to induce immunogenic cell death and tumour antigen release and to reprogramme the immunosuppressive tumour microenvironment. Five phase III trials have thus far shown improvements in progression-free survival and objective response rates with regimens combining ICIs with transarterial chemoembolization. Early-phase and real-world data also support the combination of ICIs with transarterial radioembolization or external beam radiotherapy, particularly in selected patients with focal disease unsuitable for embolization or portal vein tumour thrombosis. However, various challenges remain, including patient selection and therapy sequencing, response assessment, and the growing difficulties in demonstrating survival advantages in the context of widespread post-progression crossover and effective subsequent therapies. In this Review, we describe the biological rationale, summarize the clinical evidence and discuss future directions for trial design, imaging and biomarker-driven patient selection in the integration of LRTs with immune-based regimens for patients with unresectable hepatocellular carcinoma. CI - (c) 2026. Springer Nature Limited. FAU - Cioni, Dania AU - Cioni D AUID- ORCID: 0000-0002-5120-886X AD - Department of Radiology, Pisa University School of Medicine, Pisa, Italy. FAU - Mauro, Ezequiel AU - Mauro E AUID- ORCID: 0000-0002-0757-7676 AD - Liver Cancer Translational Research Group - IDIBAPS, Liver Unit - Hospital Clinic, Universitat de Barcelona, Barcelona, Spain. FAU - Feng, Dan AU - Feng D AD - Division of Oncology, Icahn School of Medicine at Mount Sinai, New York, NY, USA. FAU - Cano-Segarra, Guillem AU - Cano-Segarra G AD - Liver Cancer Translational Research Group - IDIBAPS, Liver Unit - Hospital Clinic, Universitat de Barcelona, Barcelona, Spain. AD - Mount Sinai Liver Cancer Program, Division of Liver Diseases, Department of Medicine, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. FAU - Kim, Edward AU - Kim E AUID- ORCID: 0000-0001-8050-2366 AD - Division of Interventional Radiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA. FAU - Dekervel, Jeroen AU - Dekervel J AD - Digestive Oncology, Department of Gastroenterology/Digestive Oncology, University Hospitals Leuven, Leuven, Belgium. FAU - Ronot, Maxime AU - Ronot M AD - Department of Radiology, MOSAIC2, Hospital Beaujon AP-HP Nord, Clichy, France. AD - Universite Paris-Cite, Centre de recherche sur l'inflammation, UMR, Paris, France. FAU - Fanni, Salvatore Claudio AU - Fanni SC AUID- ORCID: 0000-0002-4003-3320 AD - Clinical and Translational Science PhD Program, Pisa University School of Medicine, Pisa, Italy. FAU - Lencioni, Riccardo AU - Lencioni R AD - Department of Radiology, Pisa University School of Medicine, Pisa, Italy. riccardo.lencioni@unipi.it. FAU - Llovet, Josep M AU - Llovet JM AUID- ORCID: 0000-0003-0547-2667 AD - Liver Cancer Translational Research Group - IDIBAPS, Liver Unit - Hospital Clinic, Universitat de Barcelona, Barcelona, Spain. josep.llovet@mountsinai.org. AD - Mount Sinai Liver Cancer Program, Division of Liver Diseases, Department of Medicine, Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. josep.llovet@mountsinai.org. AD - Institucio Catalana de Recerca i Estudis Avancats, Barcelona, Spain. josep.llovet@mountsinai.org. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - Nat Rev Clin Oncol JT - Nature reviews. Clinical oncology JID - 101500077 SB - IM COIS- Competing interests: J.M.L. is receiving consulting fees from AbbVie, AstraZeneca, Eisai Inc., Bayer HealthCare Pharmaceuticals, B.I., Bristol Myers Squibb, Genentech Roche, Glycotest Inc., Merck and Sanofi Aventis; and is receiving research support from Eisai Inc. and Genentech Roche. The other authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:31 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/30 00:31 [entrez] AID - 10.1038/s41571-026-01210-6 [pii] AID - 10.1038/s41571-026-01210-6 [doi] PST - aheadofprint SO - Nat Rev Clin Oncol. 2026 Oct 28. doi: 10.1038/s41571-026-01210-6. PMID- 42957892 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1432-0614 (Electronic) IS - 0175-7598 (Linking) VI - 110 IP - 1 DP - 2026 Aug 3 TI - Metabolic responses of microbial consortia to winery wastewater-associated inhibitors. LID - 322 [pii] LID - 10.1007/s00253-026-13983-y [doi] AB - This study evaluates how non-conventional anaerobic microbial consortia from sanitary landfill leachate and mining wastewater respond to selected winery wastewater-associated inhibitors, including alcohols, carboxylic acids, and phenolic compounds. Batch assays were conducted in nutrient broth acetate-fumarate medium under single and combined inhibitory conditions. The enriched consortia were characterized by full-length 16S rRNA gene sequencing. Both consortia exhibited comparable growth rates under control conditions, but showed distinct growth dynamics, fumarate consumption, succinate and acetate profiles, and inhibitor consumption patterns. The landfill-derived consortium was dominated by Escherichia and displayed faster fumarate consumption under control and alcohol-supplemented conditions, indicating higher baseline substrate turnover. In contrast, the mining-derived consortium showed a more distributed taxonomic profile, dominated by Proteus, Wolinella, and Escherichia, and maintained fumarate consumption more consistently under carboxylic acid and phenolic stress. Alcohols had limited effects on growth and fumarate consumption, whereas carboxylic acids significantly reduced growth and altered metabolite profiles. Phenolic compounds exerted a strong inhibitory effect, particularly at high concentrations. Catechol and tyrosol showed only minor concentration changes, consistent with low apparent consumption under the tested anaerobic conditions. Under combined inhibitory conditions, both consortia maintained fumarate consumption and metabolite formation despite reduced growth, with the mining-derived consortium showing increased fumarate consumption and enhanced succinate production. Microbial source, enrichment history, community composition, and inhibitory conditions jointly shaped the resilience and metabolic performance of the anaerobic consortia. This work supports screening non-conventional microbial consortia for future anaerobic and bioelectrochemical treatment of chemically complex agro-industrial wastewater. KEY POINTS: * Non-conventional anaerobic consortia maintained activity under inhibition * Taxonomic composition was associated with distinct metabolic profiles * Phenolics caused strong inhibition and low apparent anaerobic consumption. CI - (c) 2026. The Author(s). FAU - Baia, Ana AU - Baia A AD - Fiber Materials and Environmental Technologies (FibEnTech-UBI), Universidade da Beira Interior, Covilha, Portugal. AD - Department of Chemistry, Universidade da Beira Interior, Covilha, Portugal. FAU - Arroyo-Escoto, Alonso I AU - Arroyo-Escoto AI AD - Alentejo Biotechnology Center for Agriculture and Agro-Food (CEBAL), Polytechnic Institute of Beja (IPBeja), Beja, Portugal. AD - MED - Mediterranean Institute for Agriculture, Environment and Development & CHANGE - Global Change and Sustainability Institute, CEBAL- Alentejo Biotechnology Center for Agriculture and Agro-Food, Beja, Portugal. AD - MED - Mediterranean Institute for Agriculture, Environment and Development & CHANGE- Global Change and Sustainability Institute, Institute for Advanced Studies and Research (IIFA), University of Evora, Evora, Portugal. FAU - Abdelkarim, Bilel AU - Abdelkarim B AD - Fiber Materials and Environmental Technologies (FibEnTech-UBI), Universidade da Beira Interior, Covilha, Portugal. FAU - Ramos, Nuno AU - Ramos N AD - Department of Chemistry, Universidade da Beira Interior, Covilha, Portugal. FAU - Afonso, Cesar AU - Afonso C AD - Fiber Materials and Environmental Technologies (FibEnTech-UBI), Universidade da Beira Interior, Covilha, Portugal. AD - Department of Chemistry, Universidade da Beira Interior, Covilha, Portugal. FAU - Pesce, Gaetano R AU - Pesce GR AD - Department of Agronomy, Food, Natural Resources, Animals and Environment-DAFNAE, University of Padua, Agripolis Campus, Legnaro, PD, Italy. FAU - Fernandes, Maria C AU - Fernandes MC AUID- ORCID: 0000-0003-1977-8771 AD - Alentejo Biotechnology Center for Agriculture and Agro-Food (CEBAL), Polytechnic Institute of Beja (IPBeja), Beja, Portugal. maria.fernandes@cebal.pt. AD - MED - Mediterranean Institute for Agriculture, Environment and Development & CHANGE - Global Change and Sustainability Institute, CEBAL- Alentejo Biotechnology Center for Agriculture and Agro-Food, Beja, Portugal. maria.fernandes@cebal.pt. FAU - Fernandes, Annabel AU - Fernandes A AUID- ORCID: 0000-0002-9007-5342 AD - Fiber Materials and Environmental Technologies (FibEnTech-UBI), Universidade da Beira Interior, Covilha, Portugal. annabelf@ubi.pt. AD - Department of Chemistry, Universidade da Beira Interior, Covilha, Portugal. annabelf@ubi.pt. LA - eng GR - 2022.11077.BD/Fundacao para a Ciencia e a Tecnologia/ GR - UI/BD/153579/2022/Fundacao para a Ciencia e a Tecnologia/ GR - 2022.02566.PTDC/Fundacao para a Ciencia e a Tecnologia/ GR - UIDB/05183/2025/Fundacao para a Ciencia e a Tecnologia/ GR - UID/00195/2025/Fundacao para a Ciencia e a Tecnologia/ GR - ALT2030-FEDER-01951700/European Regional Development Fund/ PT - Journal Article DEP - 20260803 PL - Germany TA - Appl Microbiol Biotechnol JT - Applied microbiology and biotechnology JID - 8406612 RN - 0 (Wastewater) RN - 0 (RNA, Ribosomal, 16S) RN - 0 (Phenols) RN - 0 (Carboxylic Acids) RN - 0 (Fumarates) RN - 0 (Alcohols) RN - AB6MNQ6J6L (Succinic Acid) RN - 0 (Industrial Waste) SB - IM MH - *Wastewater/microbiology/chemistry MH - *Microbial Consortia/drug effects/physiology MH - Anaerobiosis MH - RNA, Ribosomal, 16S/genetics MH - *Bacteria/metabolism/genetics/classification/drug effects/growth & development MH - Phenols/pharmacology MH - Carboxylic Acids/pharmacology/metabolism MH - *Wine MH - Fumarates/metabolism MH - Alcohols/pharmacology MH - Succinic Acid/metabolism MH - Industrial Waste OTO - NOTNLM OT - 16S rRNA gene sequencing OT - Agro-industrial effluents OT - Anaerobic consortia OT - Fumarate reduction OT - Inhibitory compounds OT - Metabolic resilience COIS- Declarations. Ethical approval: Research does not involve human participants (including data/samples) or animal subjects. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:31 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/25 00:00 [received] PHST- 2026/07/23 00:00 [accepted] PHST- 2026/07/21 00:00 [revised] PHST- 2026/10/30 00:31 [entrez] AID - 10.1007/s00253-026-13983-y [pii] AID - 10.1007/s00253-026-13983-y [doi] PST - epublish SO - Appl Microbiol Biotechnol. 2026 Aug 3;110(1):322. doi: 10.1007/s00253-026-13983-y. PMID- 42957885 OWN - NLM STAT- Publisher LR - 20261030 IS - 1476-4660 (Electronic) IS - 1476-1122 (Linking) DP - 2026 Oct 29 TI - Free-standing two-dimensional metal oxides at the single-atomic-layer limit. LID - 10.1038/s41563-026-02793-2 [doi] AB - Two-dimensional metal oxides offer a versatile platform for exploring quantum phenomena and enable functionalities beyond those of their bulk counterparts. Despite advances in synthesizing ultrathin and free-standing oxides, controlled fabrication at the single-atomic-layer limit has remained a formidable challenge. Here we report the controlled fabrication of diverse free-standing two-dimensional metal oxides (MO), including CrO, ErO and BiO, down to the single-atomic-layer limit via electron-beam-induced dehalogenation of layered metal oxyhalides (MOX). Using monolayer CrO as a representative system, which features a single-atomic-layer square lattice, we obtain free-standing single-crystalline domains exceeding 10(4) nm(2) and demonstrate controllable patterning at the micrometre scale. Density functional theory confirms both the thermodynamic and kinetic stability of free-standing monolayer CrO, predicting a Neel antiferromagnetic order below 230 K. Combined experimental and theoretical analysis reveals that ionization-driven halogen selective removal and lattice relaxation underpin the CrOCl-to-CrO transformation, a conversion mechanism potentially extendable to other metal oxyhalides. This approach further enables the fabrication of MOX-MO lateral heterostructures as well as aligned and twisted metal oxide bilayers, which exhibit pronounced excitonic responses with an unusually large exciton binding energy. Our work opens opportunities for two-dimensional metal oxide monolayers, heterostructures and moire superlattices, and for exploring emergent excitonic phenomena in low-dimensional oxides. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature Limited. FAU - Gao, Meng AU - Gao M AUID- ORCID: 0000-0003-4719-7536 AD - School of Physical Sciences, University of Chinese Academy of Sciences, Beijing, China. FAU - Guo, Deping AU - Guo D AD - Key Laboratory of Quantum State Construction and Manipulation (Ministry of Education), School of Physics, Renmin University of China, Beijing, China. AD - College of Physics and Electronic Engineering, Center for Computational Sciences, Sichuan Normal University, Chengdu, China. FAU - Li, Ang AU - Li A AUID- ORCID: 0009-0002-5967-0626 AD - School of Physical Sciences, University of Chinese Academy of Sciences, Beijing, China. FAU - Wang, Renhong AU - Wang R AD - Key Laboratory of Quantum State Construction and Manipulation (Ministry of Education), School of Physics, Renmin University of China, Beijing, China. FAU - Jin, Zhitong AU - Jin Z AD - School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai, China. FAU - Huang, Chun AU - Huang C AD - School of Integrated Circuits and Electronics & School of Multidisciplinary Science & School of Physics, Beijing Institute of Technology, Beijing, China. FAU - Lu, Yixiang AU - Lu Y AUID- ORCID: 0009-0001-5048-8345 AD - School of Physical Sciences, University of Chinese Academy of Sciences, Beijing, China. FAU - Pan, Yuhao AU - Pan Y AD - Key Laboratory of Quantum State Construction and Manipulation (Ministry of Education), School of Physics, Renmin University of China, Beijing, China. FAU - Dai, Jiuxiang AU - Dai J AUID- ORCID: 0009-0008-2226-226X AD - School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai, China. FAU - Huang, Panqi AU - Huang P AD - School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai, China. FAU - Liu, Kangjie AU - Liu K AD - School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai, China. FAU - Wang, Cong AU - Wang C AUID- ORCID: 0000-0002-5297-9586 AD - Key Laboratory of Quantum State Construction and Manipulation (Ministry of Education), School of Physics, Renmin University of China, Beijing, China. FAU - Bao, Lihong AU - Bao L AUID- ORCID: 0000-0002-2942-892X AD - School of Physical Sciences, University of Chinese Academy of Sciences, Beijing, China. AD - Institute of Physics, Chinese Academy of Sciences, Beijing, China. FAU - Zhou, Lin AU - Zhou L AD - School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai, China. FAU - Qiao, Jingsi AU - Qiao J AUID- ORCID: 0000-0001-6464-5500 AD - School of Integrated Circuits and Electronics & School of Multidisciplinary Science & School of Physics, Beijing Institute of Technology, Beijing, China. FAU - Ji, Wei AU - Ji W AUID- ORCID: 0000-0001-5249-6624 AD - Key Laboratory of Quantum State Construction and Manipulation (Ministry of Education), School of Physics, Renmin University of China, Beijing, China. wji@ruc.edu.cn. FAU - Pennycook, Stephen J AU - Pennycook SJ AUID- ORCID: 0000-0002-3210-6323 AD - School of Physical Sciences, University of Chinese Academy of Sciences, Beijing, China. stevepennycook@ucas.ac.cn. FAU - Gao, Hong-Jun AU - Gao HJ AUID- ORCID: 0000-0001-9323-1307 AD - School of Physical Sciences, University of Chinese Academy of Sciences, Beijing, China. hjgao@iphy.ac.cn. AD - Institute of Physics, Chinese Academy of Sciences, Beijing, China. hjgao@iphy.ac.cn. FAU - Zhou, Wu AU - Zhou W AUID- ORCID: 0000-0002-6803-1095 AD - School of Physical Sciences, University of Chinese Academy of Sciences, Beijing, China. wuzhou@ucas.ac.cn. LA - eng GR - YSBR-003/Chinese Academy of Sciences (CAS)/ GR - BJJWZYJH01201914430039/Beijing Municipal Commission of Education (Beijing Municipal Education Commission)/ GR - 52103344/National Natural Science Foundation of China (National Science Foundation of China)/ GR - 92477205/National Natural Science Foundation of China (National Science Foundation of China)/ PT - Journal Article DEP - 20261029 PL - England TA - Nat Mater JT - Nature materials JID - 101155473 SB - IM COIS- Competing interests: A Chinese patent that covers this subtractive fabrication method of 2D oxides was filed by the University of Chinese Academy of Sciences. The patent application is pending (no. 202511296642.5), and the inventor names are W.Z. and M.G. The other authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:31 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/10/18 00:00 [received] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/30 00:31 [entrez] AID - 10.1038/s41563-026-02793-2 [pii] AID - 10.1038/s41563-026-02793-2 [doi] PST - aheadofprint SO - Nat Mater. 2026 Oct 29. doi: 10.1038/s41563-026-02793-2. PMID- 42957881 OWN - NLM STAT- Publisher LR - 20261030 IS - 1469-3178 (Electronic) IS - 1469-221X (Linking) DP - 2026 Oct 29 TI - PCGF1/PRC1.1 selectively sustains astroglial lineage progression by repressing N4bp1 to stabilize Notch signaling. LID - 10.1038/s44319-026-00983-2 [doi] AB - The generation of distinct neural lineages from common progenitors requires coordinated regulation by developmental signaling pathways and epigenetic mechanisms. Although extracellular signals such as Notch promote astrocyte differentiation, how epigenetic mechanisms establish the competence of progenitors to respond to these cues remains poorly understood. Here, we identify PCGF1 (NSPC1), a core component of the non-canonical Polycomb repressive complex PRC1.1, as an epigenetic regulator of cortical astroglial lineage progression. Conditional deletion of Pcgf1 in the dorsal forebrain selectively impairs astrocyte generation without affecting neurogenesis, cortical lamination, or the abundance of EGFR-positive gliogenic progenitors. Pcgf1 deficiency impairs the progression toward astroglial-associated states and reduces SOX9 + , BLBP + , GFAP + , and ALDH1L1+ populations. Mechanistically, PCGF1 directly represses N4bp1, a negative regulator of the Notch intracellular domain (NICD). Loss of Pcgf1 leads to N4BP1 upregulation, NICD destabilization, and attenuation of Notch signaling, whereas N4bp1 deletion produces the opposite astroglial phenotype. Our findings reveal a PRC1.1-dependent mechanism that promotes astroglial lineage progression by sustaining developmental signaling output, establishing the PCGF1-N4BP1-Notch axis as a molecular link between chromatin regulation and neural lineage diversification. CI - (c) 2026. The Author(s). FAU - Yin, Luyao AU - Yin L AUID- ORCID: 0009-0005-6205-7955 AD - State Key Laboratory of Common Mechanism Research for Major Diseases, Department of Biochemistry & Molecular Biology, Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Zhou, Jiaqi AU - Zhou J AUID- ORCID: 0009-0004-5802-5697 AD - State Key Laboratory of Common Mechanism Research for Major Diseases, Department of Biochemistry & Molecular Biology, Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. AD - School of Basic Medical Sciences, Dali University, Dali, China. FAU - Ma, Ziqi AU - Ma Z AD - State Key Laboratory of Common Mechanism Research for Major Diseases, Department of Biochemistry & Molecular Biology, Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Wang, Chunhui AU - Wang C AD - State Key Laboratory of Common Mechanism Research for Major Diseases, Department of Biochemistry & Molecular Biology, Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Shen, Mengjia AU - Shen M AD - State Key Laboratory of Common Mechanism Research for Major Diseases, Department of Biochemistry & Molecular Biology, Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Hou, Lin AU - Hou L AD - State Key Laboratory of Common Mechanism Research for Major Diseases, Department of Biochemistry & Molecular Biology, Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Yin, Bin AU - Yin B AD - State Key Laboratory of Common Mechanism Research for Major Diseases, Department of Biochemistry & Molecular Biology, Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Qiang, Boqin AU - Qiang B AD - State Key Laboratory of Common Mechanism Research for Major Diseases, Department of Biochemistry & Molecular Biology, Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Shu, Pengcheng AU - Shu P AUID- ORCID: 0000-0002-3091-2559 AD - State Key Laboratory of Common Mechanism Research for Major Diseases, Department of Biochemistry & Molecular Biology, Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. pengcheng_shu@ibms.pumc.edu.cn. FAU - Peng, Xiaozhong AU - Peng X AUID- ORCID: 0000-0002-9592-9554 AD - State Key Laboratory of Common Mechanism Research for Major Diseases, Department of Biochemistry & Molecular Biology, Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. pengxiaozhong@pumc.edu.cn. AD - Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, China. pengxiaozhong@pumc.edu.cn. LA - eng GR - 2021ZD0200900/National Science and Techlonogy Innovation 2030 Grants of China/ GR - 32370883/MOST | National Natural Science Foundation of China (NSFC)/ GR - 5262021/Beijing Natural Science Foundation/ GR - 2060204/State Key Laboratory of Special Fund/ GR - 2025-I2M-XHZY-021/CAMS | Chinese Academy of Medical Sciences Initiative for Innovative Medicine ( )/ GR - 202605AF350018/Yunnan Provincial Science and Technology Talent and Platform Program/ PT - Journal Article DEP - 20261029 PL - England TA - EMBO Rep JT - EMBO reports JID - 100963049 SB - IM COIS- Disclosure and competing interests statement. The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:30 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/01 00:00 [received] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/09/19 00:00 [revised] PHST- 2026/10/30 00:30 [entrez] AID - 10.1038/s44319-026-00983-2 [pii] AID - 10.1038/s44319-026-00983-2 [doi] PST - aheadofprint SO - EMBO Rep. 2026 Oct 29. doi: 10.1038/s44319-026-00983-2. PMID- 42957879 OWN - NLM STAT- Publisher LR - 20261030 IS - 1469-3178 (Electronic) IS - 1469-221X (Linking) DP - 2026 Oct 28 TI - TRAP complex is essential for coronavirus replication and glycoprotein biogeneses. LID - 10.1038/s44319-026-00986-z [doi] AB - Coronaviruses remodel the endoplasmic reticulum (ER) to build replication organelles while producing heavily glycosylated membrane proteins, but the mechanisms coordinating these demands are unclear. Here, we describe the ER-resident translocon-associated protein (TRAP/SSR) complex as a conserved host dependency across diverse coronaviruses. Using porcine hemagglutinating encephalomyelitis virus (PHEV) as a model, we identify TRAPgamma in a genome-wide CRISPR-Cas9 screen as essential for productive viral infection. TRAPgamma depletion suppresses viral replication, disrupts canonical replication organelle formation, and amplifies innate immune signaling. Viral glycoproteins (nsp4, Spike, and hemagglutinin-esterase) selectively engage specific TRAP subunits (alpha, beta, gamma, and delta), and reducing their signal-sequence hydrophobicity compromises glycoprotein production and N-glycosylation, consistent with impaired co-translational ER targeting or translocation. Inhibition of the oligosaccharyltransferase (OST) complex recapitulates these defects, supporting a sequential signal recognition particle (SRP)-TRAP-OST axis. Thus, our findings reveal an essential role for the TRAP complex in viral subversion of ER proteostasis, highlighting ER co‑translational translocation machinery as a pivotal host-pathogen interface during the viral lifecycle. CI - (c) 2026. The Author(s). FAU - Tian, Yihan AU - Tian Y AUID- ORCID: 0009-0008-0451-3205 AD - State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Shi, Junchao AU - Shi J AUID- ORCID: 0000-0003-0989-0495 AD - State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Mu, Shaoqian AU - Mu S AD - State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, China. FAU - Wei, Hanlu AU - Wei H AD - State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, China. FAU - He, Wenqi AU - He W AUID- ORCID: 0000-0003-3254-1242 AD - State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, China. hewq@jlu.edu.cn. FAU - Li, Zi AU - Li Z AUID- ORCID: 0000-0003-3968-9303 AD - State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, and College of Veterinary Medicine, Jilin University, Changchun, China. lizi@jlu.edu.cn. LA - eng GR - 32272956/MOST | National Natural Science Foundation of China (NSFC)/ GR - 32522102/MOST | National Natural Science Foundation of China (NSFC)/ GR - 32473011/MOST | National Natural Science Foundation of China (NSFC)/ GR - JJKH20250161BS/Science and Technology Research Project of the Education Department of Jilin Province/ PT - Journal Article DEP - 20261028 PL - England TA - EMBO Rep JT - EMBO reports JID - 100963049 SB - IM COIS- Disclosure and competing interests statement. The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:30 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/16 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/30 00:30 [entrez] AID - 10.1038/s44319-026-00986-z [pii] AID - 10.1038/s44319-026-00986-z [doi] PST - aheadofprint SO - EMBO Rep. 2026 Oct 28. doi: 10.1038/s44319-026-00986-z. PMID- 42957874 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261030 IS - 1436-5073 (Electronic) IS - 0026-3672 (Linking) VI - 193 IP - 11 DP - 2026 Oct 30 TI - A carbon dots-doped europium-MOF for dual-signal ratiometric fluorescence and colorimetric detection of flumequine. LID - 770 [pii] LID - 10.1007/s00604-026-08484-8 [doi] AB - A signal probe, Eu-2MIM@CDs, was prepared by embedding carbon dots (CDs) into a porous europium-based metal-organic framework (Eu-2MIM) composed of europium and 2-methylimidazole, and was deployed for the dual-signal detection of flumequine (FLU) antibiotic. This single probe provided two distinct signal outputs for FLU ratiometric sensing: colorimetric and fluorescence signals. In the colorimetric channel, the Eu-2MIM@CDs complex reacted with Eriochrome Black T (EBT) to form a magenta Eu(3)(+)-EBT complex. Upon the introduction of FLU, FLU competitively displaced EBT from the complex, releasing free EBT and restoring the blue color. This displacement process was accompanied by ratiometric absorbance changes at 538 nm and 610 nm, enabling multi-color visual detection. In the fluorescence channel, the Eu-2MIM@CDs probe initially exhibited emission from CDs at 436 nm. Upon adding FLU, the characteristic Eu(3)(+) fluorescence was significantly enhanced through a coordination-environment change, which simultaneously quenched the emission at 436 nm and generated a new emission peak at 612 nm, yielding a concentration-dependent ratiometric fluorescence response for FLU detection. In the colorimetric channel, as the FLU concentration increased, the absorbance at 538 nm progressively decreased while that at 610 nm increased, producing a well-defined ratiometric colorimetric signal. This channel provided a wide linear detection range from 0.01 to 2.5 microM with a detection limit of 3.3 nM. The visible color of the solution shifted from magenta to blue with rising FLU concentration. In the fluorescence channel, the intensity at 436 nm gradually decreased along with an increase at 612 nm, showing a linear range from 0.005 to 2.0 microM and a detection limit of 1.7 nM. The fluorescence response was rapid, completing within 1 min. As the FLU concentration increased, a visible color shifted from blue to red. The fluorescence channel exhibited high selectivity toward FLU over other antibiotics, organic compounds and metal ions, while the colorimetric channel showed fluoroquinolone-class selectivity. Furthermore, the probe showed good practicability in actual samples. Collectively, this dual-signal probe provides a simple, fast and efficient method for both visual and quantitative FLU detection in complex matrices. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature. FAU - Yi, Jintao AU - Yi J AD - Key Laboratory of Synthetic Pharmaceutical Chemistry of Jiangxi Province, Gannan Normal University, Ganzhou, 341000, China. yistarthappy@163.com. FAU - Li, Anqi AU - Li A AD - Key Laboratory of Synthetic Pharmaceutical Chemistry of Jiangxi Province, Gannan Normal University, Ganzhou, 341000, China. FAU - Han, Xianqin AU - Han X AD - Key Laboratory of Synthetic Pharmaceutical Chemistry of Jiangxi Province, Gannan Normal University, Ganzhou, 341000, China. FAU - Fu, Nani AU - Fu N AD - Key Laboratory of Synthetic Pharmaceutical Chemistry of Jiangxi Province, Gannan Normal University, Ganzhou, 341000, China. FAU - Chen, Ziluo AU - Chen Z AD - Key Laboratory of Synthetic Pharmaceutical Chemistry of Jiangxi Province, Gannan Normal University, Ganzhou, 341000, China. FAU - Yang, Tingting AU - Yang T AD - Key Laboratory of Synthetic Pharmaceutical Chemistry of Jiangxi Province, Gannan Normal University, Ganzhou, 341000, China. FAU - Zhou, Zihan AU - Zhou Z AD - Key Laboratory of Synthetic Pharmaceutical Chemistry of Jiangxi Province, Gannan Normal University, Ganzhou, 341000, China. FAU - Wang, Min AU - Wang M AD - Key Laboratory of Synthetic Pharmaceutical Chemistry of Jiangxi Province, Gannan Normal University, Ganzhou, 341000, China. FAU - Chen, Yashan AU - Chen Y AD - Key Laboratory of Synthetic Pharmaceutical Chemistry of Jiangxi Province, Gannan Normal University, Ganzhou, 341000, China. FAU - Xue, Jun AU - Xue J AD - Key Laboratory of Synthetic Pharmaceutical Chemistry of Jiangxi Province, Gannan Normal University, Ganzhou, 341000, China. FAU - Ma, Xiaoming AU - Ma X AD - Key Laboratory of Synthetic Pharmaceutical Chemistry of Jiangxi Province, Gannan Normal University, Ganzhou, 341000, China. AD - Institute for Quality & Safety and Standards of Agricultural Products, Jiangxi Academy of Agricultural Sciences, Nanchang, 330200, China. FAU - Qian, Yiping AU - Qian Y AD - Key Laboratory of Synthetic Pharmaceutical Chemistry of Jiangxi Province, Gannan Normal University, Ganzhou, 341000, China. LA - eng GR - 20242BAB20091/the Jiangxi Provincial Natural Science Foundation/ GR - tzb2026017/the First Batch of Key University-Level Incubation Projects for the 20th "Challenge Cup" Competition of Gannan Normal University/ GR - KL-KF-202403/the Open Project Program of Key Laboratory of Institute for Quality & Safety and Standards of Agricultural Products Research Jiangxi Academy of Agricultural Sciences/ GR - JXYJG-2024-119/the Jiangxi Provincial Degree and Graduate Education Teaching Research Reform Project/ PT - Journal Article DEP - 20261030 PL - Austria TA - Mikrochim Acta JT - Mikrochimica acta JID - 7808782 SB - IM OTO - NOTNLM OT - Colorimetric detection OT - Eu-2MIM@CDs OT - Flumequine antibiotics OT - Fluorescence detection COIS- Declarations. Competing interests: The authors declare no competing interests. Artificial intelligence statement: No artificial intelligence (AI) tools were used in the preparation of this manuscript. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:30 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/07/15 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/30 00:30 [entrez] AID - 10.1007/s00604-026-08484-8 [pii] AID - 10.1007/s00604-026-08484-8 [doi] PST - epublish SO - Mikrochim Acta. 2026 Oct 30;193(11):770. doi: 10.1007/s00604-026-08484-8. PMID- 42957866 OWN - NLM STAT- Publisher LR - 20261030 IS - 2190-3883 (Electronic) IS - 1234-1983 (Linking) DP - 2026 Oct 30 TI - Integrated genomic, immunoinformatic and experimental evaluation of conserved pneumococcal surface proteins for serotype-independent vaccine development. LID - 10.1007/s13353-026-01127-x [doi] AB - BACKGROUND: Pneumococcal conjugate vaccines (PCVs), which target capsular polysaccharides, have significantly reduced the burden of invasive pneumococcal disease; however, their effectiveness is limited by serotype replacement, geographic variation, and the emergence of non-vaccine serotypes. METHODS: Pan-genome analysis was conducted to identify the conserved core genes of S. pneumoniae. An integrated bioinformatics approach, incorporating PSORTb, SignalP, TMHMM, and domain-based motif analysis, predicted Surface-associated proteins. Highly conserved Surface-associated proteins were further evaluated for their gene expression in vitro infection of A549 lung epithelial cells using RT-qPCR, and surface accessibility assessment by flow cytometry. Further immunoinformatics analyses for their antigenicity (VaxiJen), linear B-cell epitope prediction (BepiPred 3.0), and MHC-II (HLA-DR) binding prediction was perfoemd. RESULTS: Four surface-associated lipoproteins-PsaA, AdcA, AmiA, and Lmb-were universally conserved and predicted to be surface-exposed. During epithelial cell infection, all four genes were actively expressed, with psaA showed greater early upregulation (~ 2.5-fold; p < 0.05). Flow cytometry confirmed surface localization of all proteins, with PsaA exhibiting the highest accessibility (92% positive cells; highest MFI). Immunoinformatics analyses demonstrated strong antigenicity scores, enriched linear B-cell epitope clusters, and multiple high-affinity MHC-II binding peptides for these proteins, particularly PsaA and AdcA. In contrast, conserved multi-pass transmembrane proteins (e.g., SecG, MisCA, EcfT) showed limited surface accessibility. CONCLUSIONS: The study identifies conserved pneumococcal surface proteins that could be used as a vaccine candidate. PsaA is the most promising serotype-independent vaccine candidate, while other AdcA, AmiA, and Lmb represent potential antigens for multicomponent formulations. CI - (c) 2026. The Author(s), under exclusive licence to Institute of Plant Genetics Polish Academy of Sciences. FAU - Gopalakrishnan, Sangeetha AU - Gopalakrishnan S AD - Department of Medical Laboratory Technology, School of Allied Health Science, Sathyabama Institute of Science and Technology, Chennai, India. AD - Division of Laboratories, Biochemistry & Hematology Section, Central Leprosy Teaching and Research Institute, Chengalpattu, India. FAU - Jayapal, Premkumar AU - Jayapal P AD - Department of Medical Laboratory Technology, School of Allied Health Science, Sathyabama Institute of Science and Technology, Chennai, India. premkumar.biomed@sathyabama.ac.in. AD - School of Bio & Chemical Engineering, Department of Biomedical Engineering, Sathyabama Institute of Science and Technology, Chennai, India. premkumar.biomed@sathyabama.ac.in. FAU - Selvamani, Prathibha AU - Selvamani P AD - Department of Medical Laboratory Technology, School of Allied Health Science, Sathyabama Institute of Science and Technology, Chennai, India. FAU - Pavithra, Anandan AU - Pavithra A AD - Graduate Student, Department of Medical Laboratory Technology, School of Allied Health Science, Sathyabama Institute of Science and Technology, Chennai, India. FAU - Ramakrishnan, Shincy Mettingal AU - Ramakrishnan SM AD - Central Research Laboratory, Kempegowda Institute of Medical Sciences, Bengaluru, Karnataka, India. AD - Department of Biotechnology and Genetics, School of Sciences, Jain University, Bangalore, India. FAU - Ravikumar, Kadahalli Lingegowda AU - Ravikumar KL AD - Central Research Laboratory, Kempegowda Institute of Medical Sciences, Bengaluru, Karnataka, India. FAU - Parthasarathy, Krupakar AU - Parthasarathy K AD - Sathyabama Research Park, Sathyabama Institute of Science and Technology, Chennai, India. FAU - John, James AU - John J AUID- ORCID: 0000-0003-3343-8677 AD - Department of Medical Laboratory Technology, School of Allied Health Science, Sathyabama Institute of Science and Technology, Chennai, India. james_cmc2002@yahoo.co.in. AD - School of Bio & Chemical Engineering, Department of Biomedical Engineering, Sathyabama Institute of Science and Technology, Chennai, India. james_cmc2002@yahoo.co.in. LA - eng GR - IIRPSG-2024-01-01597/ICMR, New Delhi (Investigator Initiated Research ID: IIRPSG-2024-01-01597)/ PT - Journal Article DEP - 20261030 PL - England TA - J Appl Genet JT - Journal of applied genetics JID - 9514582 SB - IM OTO - NOTNLM OT - Streptococcus pneumoniae OT - conserved surface proteins OT - pneumococcal pathogenesis OT - protein-based vaccine targets OT - serotype-independent vaccines COIS- Declarations. Ethical approval: Not applicable, as this study did not involve human participants or animal experiments. Artificial intelligence (AI) use disclosure: The authors used Gemini (Google LLC) solely for language editing, grammar correction, and improvement of manuscript readability. The AI tool was not used for study conception, experimental design, data collection, data analysis, statistical analysis, and interpretation of results, literature review, figure preparation, image generation, or the development of scientific conclusions. All figures and graphical schematics presented in this manuscript were independently created by the authors using BioRender (BioRender.com) and/or standard scientific software and were subsequently formatted using Microsoft PowerPoint. No AI-generated images or copyrighted third-party figures were used. All scientific content was critically reviewed, verified, and approved by the authors, who accept full responsibility for the accuracy, originality, and integrity of the manuscript. Conflict of interest: The authors declare no competing interests. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:30 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/03 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/09/24 00:00 [revised] PHST- 2026/10/30 00:30 [entrez] AID - 10.1007/s13353-026-01127-x [pii] AID - 10.1007/s13353-026-01127-x [doi] PST - aheadofprint SO - J Appl Genet. 2026 Oct 30. doi: 10.1007/s13353-026-01127-x. PMID- 42957862 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1432-0614 (Electronic) IS - 0175-7598 (Linking) VI - 110 IP - 1 DP - 2026 Aug 3 TI - Entomopathogenic fungi as microbial platforms for tyrosine kinase-oriented inhibitor discovery. LID - 324 [pii] LID - 10.1007/s00253-026-13975-y [doi] AB - Tyrosine kinase inhibitors (TKIs) remain a major focus of targeted therapeutic discovery, yet current discovery pipelines are constrained by resistance development, limited scaffold diversity, and high attrition rates. Entomopathogenic fungi (EPF) represent an underexplored microbial resource with significant biosynthetic potential for generating kinase-relevant secondary metabolites. Recent genomic studies reveal that EPF encode diverse and largely cryptic biosynthetic gene clusters (BGCs), including polyketide synthase (PKS), non-ribosomal peptide synthetase (NRPS), and hybrid pathways capable of producing structurally complex bioactive compounds. This mini-review examines the biotechnological exploitation of EPF for TKI discovery, focusing on fungal metabolites with direct or indirect kinase-related activity, genome mining strategies, biosynthetic logic, pathway regulation, and engineering approaches for metabolite production. Although only a limited number of EPF-derived metabolites have been experimentally validated as direct tyrosine kinase inhibitors, accumulating evidence indicates that many modulate kinase-associated signalling pathways and represent promising starting points for kinase-oriented natural product discovery. Particular emphasis is placed on pathway activation, heterologous expression, metabolic engineering, and scalable bioprocess development. Key translational challenges, including selectivity, toxicity, yield instability, and regulatory considerations, are also discussed. Collectively, this review highlights EPF as promising microbial platforms for kinase-oriented natural product discovery and provides a biotechnology-focused framework for advancing fungal secondary metabolites toward translational development. KEY POINTS: * Entomopathogenic fungi encode diverse kinase-active secondary metabolites. * Genome mining and synthetic biology unlock cryptic TKI pathways. * Biotechnological optimization is critical for scalable fungal TKI discovery. CI - (c) 2026. The Author(s). FAU - Chigozie, Victor Uchenna AU - Chigozie VU AUID- ORCID: 0000-0003-4139-0124 AD - Department of Pharmaceutical Microbiology and Biotechnology, David Umahi Federal University of Health Sciences, Ebonyi, Uburu, Nigeria. chigozievu@dufuhs.edu.ng. AD - NaPBiTReG (Natural Products Bioprospecting and Translation Research Group), International Institute for Pharmaceutical Research and Innovation (IIPRI), Uburu, Ebonyi, Nigeria. chigozievu@dufuhs.edu.ng. AD - Department of Pharmaceutical Microbiology and Biotechnology, Olivia University, Mukaza, Bujumbura, Burundi. chigozievu@dufuhs.edu.ng. FAU - Ogunwa, Kennedy Izuchukwu AU - Ogunwa KI AUID- ORCID: 0009-0008-4838-6375 AD - Department of Industrial and Medicinal Chemistry, David Umahi Federal University of Health Sciences, Uburu, Nigeria. AD - International Institute for Toxicology, Environmental & Occupational Health and Safety Research, DUFUHS, Ebonyi, Nigeria. FAU - Osuafor, Nkeiruka Grace AU - Osuafor NG AUID- ORCID: 0000-0001-7559-8199 AD - Department of Clinical Pharmacy and Pharmacy Administration, University of Abuja, FCT, Abuja, Nigeria. FAU - Nduwimana, Leslie Luxe AU - Nduwimana LL AUID- ORCID: 0009-0002-8693-7138 AD - Department of Pharmaceutical Microbiology and Biotechnology, Olivia University, Mukaza, Bujumbura, Burundi. FAU - Oraekei, Daniel Ikechukwu AU - Oraekei DI AUID- ORCID: 0000-0002-6313-4087 AD - Department of Pharmacology and Toxicology, Nnamdi Azikiwe University, Awka, Anambra, Nigeria. LA - eng PT - Journal Article PT - Review DEP - 20260803 PL - Germany TA - Appl Microbiol Biotechnol JT - Applied microbiology and biotechnology JID - 8406612 RN - 0 (Protein Kinase Inhibitors) RN - 0 (Biological Products) RN - 0 (Tyrosine Kinase Inhibitors) RN - EC 6.3.2.- (Peptide Synthases) RN - EC 2.7.10.1 (Protein-Tyrosine Kinases) SB - IM MH - *Protein Kinase Inhibitors/pharmacology/metabolism/isolation & purification MH - *Fungi/genetics/metabolism MH - *Drug Discovery/methods MH - Metabolic Engineering MH - Biosynthetic Pathways/genetics MH - Multigene Family MH - Biological Products/metabolism MH - Tyrosine Kinase Inhibitors MH - Animals MH - Insecta/microbiology MH - Peptide Synthases/genetics/metabolism MH - Protein-Tyrosine Kinases OTO - NOTNLM OT - Biosynthetic gene clusters OT - Entomopathogenic fungi OT - Metabolic engineering OT - Natural product drug discover OT - Secondary metabolism OT - Tyrosine kinase inhibitors COIS- Declarations. Competing interests: The authors declare no competing interests. Ethics approval: The study employed a narrative review methodology that utilized publicly available data and peer-reviewed literature. Since no human participants, animals, or private data were directly involved in this research, ethical approval was not required. However, the study adhered to ethical principles for conducting and reporting research, including transparency, accuracy, and integrity in data analysis and interpretation. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:29 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/24 00:00 [received] PHST- 2026/07/20 00:00 [accepted] PHST- 2026/07/17 00:00 [revised] PHST- 2026/10/30 00:29 [entrez] AID - 10.1007/s00253-026-13975-y [pii] AID - 10.1007/s00253-026-13975-y [doi] PST - epublish SO - Appl Microbiol Biotechnol. 2026 Aug 3;110(1):324. doi: 10.1007/s00253-026-13975-y. PMID- 42957858 OWN - NLM STAT- Publisher LR - 20261030 IS - 1476-5403 (Electronic) IS - 1350-9047 (Linking) DP - 2026 Oct 28 TI - DCST1 drives cisplatin resistance via non-canonical ubiquitination and stabilization of NFAT5 to suppress PANoptosis in bladder cancer. LID - 10.1038/s41418-026-01866-8 [doi] AB - Cisplatin-based chemotherapy constitutes the cornerstone of first-line treatment for muscle-invasive and metastatic bladder cancer (BCa); however, the frequent emergence of chemoresistance presents a formidable clinical obstacle. In the present study, by combining a systematic CRISPR-Cas9 E3 ligase library screen with drug sensitivity data from the GDSC database, DCST1 (DC-STAMP Domain Containing 1) was identified as a pivotal driver of cisplatin resistance. DCST1 expression was found to be markedly upregulated in chemoresistant BCa tissues, and its elevated expression served as an independent predictor of poor patient prognosis. Functionally, DCST1 deficiency sensitizes BCa cells to cisplatin in vitro and in vivo by triggering PANoptosis (simultaneous apoptosis, pyroptosis, and necroptosis). Mechanistically, DCST1 functions as an atypical E3 ubiquitin ligase to catalyze the K11/K27-linked polyubiquitination of NFAT5 at residue K450. This non-canonical ubiquitination stabilizes NFAT5 by preventing its lysosomal degradation and promotes its nuclear accumulation. Nuclear NFAT5 then transcriptionally activates SOD2, orchestrating an anti-PANoptotic program that ensures BCa cell survival under chemotherapeutic stress. Furthermore, through molecular docking and CETSA validation, digoxin was identified as a potent pharmacological inhibitor of NFAT5 that overcomes DCST1-mediated chemoresistance and synergistically restores cisplatin sensitivity. Collectively, these findings unveil a previously unrecognized ubiquitination-dependent mechanism of chemoresistance and establish the DCST1-NFAT5 signaling axis as a promising therapeutic target for overcoming drug resistance in BCa. CI - (c) 2026. The Author(s), under exclusive licence to ADMC Associazione Differenziamento e Morte Cellulare. FAU - Cao, Chun AU - Cao C AUID- ORCID: 0009-0004-6930-4448 AD - Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China. FAU - Lai, Yiqi AU - Lai Y AD - Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China. FAU - Ye, Wanteng AU - Ye W AD - Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China. FAU - Wang, Zihuan AU - Wang Z AD - Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China. FAU - Chen, Yuwen AU - Chen Y AD - Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China. FAU - Fan, Qin AU - Fan Q AD - School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong, PR China. FAU - Deng, Fan AU - Deng F AD - Department of Cell Biology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, PR China. FAU - Hou, Lina AU - Hou L AD - Huiqiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China. FAU - Wang, Zhizhang AU - Wang Z AUID- ORCID: 0000-0002-4699-953X AD - Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China. FAU - Tan, Wanlong AU - Tan W AD - Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China. twl@smu.edu.cn. FAU - Li, Fei AU - Li F AUID- ORCID: 0000-0002-7971-0288 AD - Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China. feili20700338@126.com. LA - eng GR - 82372867/National Natural Science Foundation of China (National Science Foundation of China)/ PT - Journal Article DEP - 20261028 PL - England TA - Cell Death Differ JT - Cell death and differentiation JID - 9437445 SB - IM COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:29 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/28 00:00 [received] PHST- 2026/08/28 00:00 [accepted] PHST- 2026/08/10 00:00 [revised] PHST- 2026/10/30 00:29 [entrez] AID - 10.1038/s41418-026-01866-8 [pii] AID - 10.1038/s41418-026-01866-8 [doi] PST - aheadofprint SO - Cell Death Differ. 2026 Oct 28. doi: 10.1038/s41418-026-01866-8. PMID- 42957855 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1432-184X (Electronic) IS - 0095-3628 (Linking) VI - 89 IP - 1 DP - 2026 Aug 3 TI - From Soil to Leaves: Decoding the Microbiome of Oil Palm in Colombia. LID - 197 [pii] LID - 10.1007/s00248-026-02844-w [doi] AB - Plant microbiomes are shaped by hierarchical ecological filters in which compartmentalization through the progressive selection of microbial communities along the soil-root-leaf continuum, emerges as the strongest structuring force. Despite the agronomic importance of oil palm (Elaeis guineensis Jacq.) as an important oil crop, compartment-resolved characterizations of its microbiome remain scarce, and the microbiome of the interspecific hybrid OxG (E. oleifera x E. guineensis), widely planted in Latin America to sustain production in disease-affected areas, had not been previously described. Here, we used 16S rRNA (V3-V4) and ITS (ITS2) metabarcoding to survey bacterial, archaeal, and fungal communities across bulk soil, rhizosphere, roots, and leaves of productive plots of E. guineensis and OxG hybrid palms under two soil consociations in Colombia. Sequencing produced 10,298,116 raw reads, of which 79.83% were assigned to amplicon sequence variants (ASVs). Compartment was the dominant force structuring both prokaryotic and fungal communities (PERMANOVA p < 0.01), with alpha diversity decreasing along the bulk soil-rhizosphere-roots-leaves continuum and the leaf compartment being the most distinct and least diverse. Compartment-specific biomarkers included Acidobacteriota and Saitozyma in soil, Bradyrhizobium and Pseudomonas in roots, and Methylocella and Pseudocladosporium in leaves. Predicted functional potential inferred with PICRUSt2 closely tracked the taxonomic gradient, with compartment explaining 85.87% of the variance in KEGG composition. Additionally, we found that OxG hybrid exhibited recurrent enrichment of Acidobacteriota and Ascomycota, along with more fragmented co-occurrence networks than E. guineensis. However, palm species effects were consistently weaker than compartment effects and varied with locality. CI - (c) 2026. The Author(s). FAU - Teheran-Sierra, Luis Guillermo AU - Teheran-Sierra LG AUID- ORCID: 0000-0002-2423-5184 AD - Colombian Oil Palm Research Center - Cenipalma, Oil Palm Agronomy Research Program, Bogota, 11121, Colombia. lgteheran@cenipalma.org. FAU - de Carvalho, Lucas Amoroso Lopes AU - de Carvalho LAL AUID- ORCID: 0000-0001-6222-4534 AD - Graduate Program in Agricultural and Livestock Microbiology, Sao Paulo State University (UNESP), Jaboticabal, SP, 14884-900, Brazil. AD - Department of Agricultural, Livestock and Environmental Biotechnology, Sao Paulo State University (UNESP), Jaboticabal, SP, 14884-900, Brazil. FAU - Mejia-Alvarado, Fernan Santiago AU - Mejia-Alvarado FS AUID- ORCID: 0000-0001-7402-6009 AD - Colombian Oil Palm Research Center - Cenipalma, Oil Palm Biology and Breeding Research Program, Bogota, 11121, Colombia. FAU - Chaves-Sierra, Catalina AU - Chaves-Sierra C AUID- ORCID: 0000-0002-7981-1118 AD - Colombian Oil Palm Research Center - Cenipalma, Oil Palm Biology and Breeding Research Program, Bogota, 11121, Colombia. FAU - Pinheiro, Daniel Guariz AU - Pinheiro DG AUID- ORCID: 0000-0001-7062-5936 AD - Department of Agricultural, Livestock and Environmental Biotechnology, Sao Paulo State University (UNESP), Jaboticabal, SP, 14884-900, Brazil. AD - Aquaculture Center of UNESP, Sao Paulo State University (UNESP), SP, Jaboticabal, Brazil. FAU - Arias, Nolver Atanacio Arias AU - Arias NAA AUID- ORCID: 0000-0001-7024-4738 AD - Colombian Oil Palm Research Center - Cenipalma, Oil Palm Agronomy Research Program, Bogota, 11121, Colombia. LA - eng PT - Journal Article DEP - 20260803 PL - United States TA - Microb Ecol JT - Microbial ecology JID - 7500663 RN - 0 (RNA, Ribosomal, 16S) SB - IM MH - Colombia MH - *Plant Leaves/microbiology MH - *Arecaceae/microbiology MH - *Bacteria/classification/genetics/isolation & purification MH - *Soil Microbiology MH - *Microbiota MH - *Fungi/classification/genetics/isolation & purification MH - RNA, Ribosomal, 16S/genetics MH - Archaea/classification/genetics/isolation & purification MH - Plant Roots/microbiology MH - Rhizosphere OTO - NOTNLM OT - Metabarcoding OT - Microbiome compartmentalization OT - OxG interspecific hybrid (Elaeis oleifera x E. guineensis) OT - Phyllosphere COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:29 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/12/16 00:00 [received] PHST- 2026/07/17 00:00 [accepted] PHST- 2026/10/30 00:29 [entrez] AID - 10.1007/s00248-026-02844-w [pii] AID - 10.1007/s00248-026-02844-w [doi] PST - epublish SO - Microb Ecol. 2026 Aug 3;89(1):197. doi: 10.1007/s00248-026-02844-w. PMID- 42957850 OWN - NLM STAT- Publisher LR - 20261030 IS - 2058-5276 (Electronic) IS - 2058-5276 (Linking) DP - 2026 Oct 29 TI - Planetary-scale metagenomic analysis reveals CRISPR targeting dynamics across ecosystems. LID - 10.1038/s41564-026-02480-7 [doi] AB - Interactions between microorganisms and their mobile genetic elements (MGEs), including viruses and plasmids, impact microbiome dynamics. CRISPR-Cas systems are important regulators of these host-MGE interactions, but a global understanding of the diversity, activity and roles of CRISPR spacers across Earth's biomes is still lacking. Here we develop a computational approach to search short-read data and collect 794 million CRISPR spacers across 367,609 public metagenomes, from which we identified 1.18 billion hits between 41 million spacers and 2.5 million viruses and plasmids. While spacers were highly variable across samples, those matching known viruses were more prevalent and conserved, consistent with a positive selection pressure associated with phage targeting. Among these, however, we find evidence suggestive of recurrent entry of phage genomes into non-host microbial cells, further blurring the line between CRISPR targeting and virus-host interactions. These results highlight key drivers of CRISPR targeting patterns likely to influence strain-level diversification and selection processes in microbiomes. CI - (c) 2026. The Regents of the University of California and Nikhil A. George, Uri Gophna, Laura A. Hug 2026. FAU - Roux, Simon AU - Roux S AUID- ORCID: 0000-0002-5831-5895 AD - DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. sroux@lbl.gov. FAU - Neri, Uri AU - Neri U AD - DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. FAU - Bushnell, Brian AU - Bushnell B AUID- ORCID: 0000-0002-8140-0131 AD - DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. FAU - Fremin, Brayon J AU - Fremin BJ AD - DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. FAU - George, Nikhil A AU - George NA AD - Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA. FAU - Gophna, Uri AU - Gophna U AUID- ORCID: 0000-0001-5129-5652 AD - The Shmunis School of Biomedicine and Cancer Research, Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel. FAU - Hug, Laura A AU - Hug LA AUID- ORCID: 0000-0001-5974-9428 AD - Department of Biology, University of Waterloo, Waterloo, Ontaria, Canada. FAU - Camargo, Antonio Pedro AU - Camargo AP AUID- ORCID: 0000-0003-3913-2484 AD - DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. FAU - Wu, Dongying AU - Wu D AD - DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. FAU - Ivanova, Natalia AU - Ivanova N AUID- ORCID: 0000-0002-5802-9485 AD - DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. FAU - Kyrpides, Nikos C AU - Kyrpides NC AUID- ORCID: 0000-0002-6131-0462 AD - DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. FAU - Eloe-Fadrosh, Emiley A AU - Eloe-Fadrosh EA AUID- ORCID: 0000-0002-8162-1276 AD - DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA. eaeloefadrosh@lbl.gov. LA - eng GR - DE-AC02-05CH11231/U.S. Department of Energy (DOE)/ GR - DE-AC02-05CH11231/U.S. Department of Energy (DOE)/ GR - DE-AC02-05CH11231/U.S. Department of Energy (DOE)/ GR - DE-AC02-05CH11231/U.S. Department of Energy (DOE)/ GR - DE-AC02-05CH11231/U.S. Department of Energy (DOE)/ GR - DE-AC02-05CH11231/U.S. Department of Energy (DOE)/ GR - DE-AC02-05CH11231/U.S. Department of Energy (DOE)/ GR - DE-AC02-05CH11231/U.S. Department of Energy (DOE)/ GR - DE-AC02-05CH11231/U.S. Department of Energy (DOE)/ GR - ERC-AdG 787514/European Commission (EC)/ GR - 2016-03686/Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (Conseil de Recherches en Sciences Naturelles et en Genie du Canada)/ PT - Journal Article DEP - 20261029 PL - England TA - Nat Microbiol JT - Nature microbiology JID - 101674869 SB - IM COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:29 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/12/22 00:00 [received] PHST- 2026/08/11 00:00 [accepted] PHST- 2026/10/30 00:29 [entrez] AID - 10.1038/s41564-026-02480-7 [pii] AID - 10.1038/s41564-026-02480-7 [doi] PST - aheadofprint SO - Nat Microbiol. 2026 Oct 29. doi: 10.1038/s41564-026-02480-7. PMID- 42957848 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 2191-1991 (Print) IS - 2191-1991 (Linking) VI - 16 IP - 1 DP - 2026 Aug 4 TI - A systematic review of economic evaluations of strategies to diagnose obstructive sleep apnoea in adults. LID - 132 [pii] LID - 10.1186/s13561-026-00823-y [doi] AB - BACKGROUND: Obstructive sleep apnoea (OSA) is a chronic condition characterised by recurrent episodes of apnoea and hypopnoea. People with untreated OSA are at increased risk of excessive daytime sleepiness (EDS), functional impairment and developing cardiovascular conditions, metabolic and neurocognitive diseases. EDS is a key determinant of quality of life (QoL) and is associated with increased healthcare utilisation and accident risk. Untreated OSA can also lead to impaired work productivity, reduced health-related quality of life (HRQoL) and life expectancy, which contribute to the UK economic burden. AIM: To review and critically appraise all published economic evaluations that assessed the cost-effectiveness of devices and strategies to diagnose and manage people with moderate-to-severe OSA. METHODS: This systematic review was conducted following the Preferred Reporting Items for Systematic Reviews guidelines and registered in the Prospective Register of Ongoing Systematic Reviews (CRD42024535732). We searched electronic databases up to March 2025. Two reviewers undertook screening, data extraction and quality appraisal using recognised reporting quality and methodological quality appraisal tools. The findings were summarised and discussed narratively. RESULTS: Twenty-nine studies were included. Fifteen studies included an economic evaluation alongside a clinical trial and 14 studies were model-based economic analyses. Although many analyses were methodologically sound in their overall approach, there were concerns regarding choice of time horizon, selection of health states, undertaking pre-model analyses and long-term adherence and efficacy of continuous positive airway pressure treatment (CPAP). Substantial methodological heterogeneity was identified across studies, including variation in diagnostic thresholds and scoring criteria used, model structures and assumptions regarding treatment adherence. Only four of twenty-nine included studies used home respiratory polygraphy (HRP) as a comparator, limiting the direct applicability of much of the evidence to the UK setting. CONCLUSION: Past economic analyses offer insight into the methods used to assess the cost-effectiveness of strategies to diagnose OSA. These economic analyses are a useful framework to build on, but clear gaps exist in the clinical and economic evidence. Future economic evaluations should prioritise contemporary diagnostic pathways and technologies, use consistent diagnostic definitions, and incorporate more realistic modelling of long-term treatment adherence and benefit. Until such information becomes available, the true cost-effectiveness of these strategies remains unknown. CI - (c) 2026. The Author(s). FAU - Osman, Aziza AU - Osman A AD - Centre for Evidence and Implementation Science, Health Services Management Centre, University of Birmingham, Birmingham, B15 2RT, UK. FAU - Auguste, Peter AU - Auguste P AD - Centre for Evidence and Implementation Science, Health Services Management Centre, University of Birmingham, Birmingham, B15 2RT, UK. p.e.auguste.1@bham.ac.uk. FAU - Court, Rachel AU - Court R AD - Centre for Evidence and Implementation Science, Health Services Management Centre, University of Birmingham, Birmingham, B15 2RT, UK. FAU - Scott, Emma AU - Scott E AD - Warwick Applied Health, Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK. FAU - Grove, Amy AU - Grove A AD - Centre for Evidence and Implementation Science, Health Services Management Centre, University of Birmingham, Birmingham, B15 2RT, UK. FAU - Ali, Asad AU - Ali A AD - University Hospitals Coventry and Warwickshire (UHCW) NHS Trust, Coventry, UK. FAU - Cappuccio, Francesco P AU - Cappuccio FP AD - Warwick Applied Health, Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK. AD - University Hospitals Coventry and Warwickshire (UHCW) NHS Trust, Coventry, UK. FAU - Miller, Michelle A AU - Miller MA AD - Warwick Applied Health, Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK. CN - FOUND study group LA - eng GR - NIHR203393/National Institute for Health and Care Research/ PT - Journal Article PT - Review DEP - 20260804 PL - Germany TA - Health Econ Rev JT - Health economics review JID - 101583209 OTO - NOTNLM OT - Diagnostic testing OT - Economic evaluation OT - Obstructive sleep apnoea OT - Systematic review COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:29 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/20 00:00 [received] PHST- 2026/07/13 00:00 [accepted] PHST- 2026/10/30 00:29 [entrez] AID - 10.1186/s13561-026-00823-y [pii] AID - 10.1186/s13561-026-00823-y [doi] PST - epublish SO - Health Econ Rev. 2026 Aug 4;16(1):132. doi: 10.1186/s13561-026-00823-y. PMID- 42957844 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2399-3642 (Electronic) IS - 2399-3642 (Linking) VI - 9 IP - 1 DP - 2026 Oct 29 TI - Metabolic sensor Gpr35 links dietary lipids to itch mediating neural circuits. LID - 1393 [pii] LID - 10.1038/s42003-026-11087-x [doi] AB - Impact of nutrition on systemic health is well-recognized but the specific sensors that couple western dietary lipid metabolites into the pathological triggers of chronic itch and inflammation remain elusive. Here, we identify a metabolic- to-sensory axis in which 9-keto-12,13-epoxy octadecenoic acid (EKODE), an endogenous dietary linoleic acid derivative acts as a potent ligand for the G protein-coupled receptor 35 (Gpr35) to drive chronic itch, inflammation, and heat hypersensitivity. We demonstrate that Gpr35 is selectively expresses in a distinct population of sensory neurons and genetic ablation of Gpr35 in sensory neurons attenuates EKODE and psoriasis-associated itch and inflammation. Furthermore, pharmacological inhibition of Gpr35 significantly attenuates itch in mouse models of psoriasis and atopic dermatitis. We show that Gpr35 signaling engages the itch-specific brain natriuretic peptide, defining a labeled-line pathway that is functionally distinct from classical nociceptive circuits. Collectively, our findings establish Gpr35 as a critical neuro-metabolic sensor in healthy and disease conditions. CI - (c) 2026. The Author(s). FAU - Wheeler, Joshua J AU - Wheeler JJ AUID- ORCID: 0000-0002-1879-1627 AD - Department of Molecular Biomedical Sciences, NC State University College of Veterinary Medicine, Raleigh, NC, USA. AD - Comparative Medicine Institute; NC State University, Raleigh, NC, USA. FAU - Lyfenko, Alla AU - Lyfenko A AD - Department of Molecular Biomedical Sciences, NC State University College of Veterinary Medicine, Raleigh, NC, USA. FAU - Zurek, Nesia A AU - Zurek NA AUID- ORCID: 0009-0003-7939-2886 AD - Department of Anesthesia, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA. FAU - Song, Keaton AU - Song K AUID- ORCID: 0009-0009-9546-4273 AD - Kimberly and Eric J. Waldman Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, NY, USA. AD - Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. AD - Marc and Jennifer Lipschultz Precision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. AD - Mark Lebwohl Center for Neuroinflammation and Sensation, Icahn School of Medicine at Mount Sinai, New York, NY, USA. AD - Allen Discovery Center for Neuroimmune Interactions, Icahn School of Medicine at Mount Sinai, New York, NY, USA. FAU - Wangzhou, Andy AU - Wangzhou A AUID- ORCID: 0000-0001-5667-1223 AD - Center for Advanced Pain Studies and Department of Neuroscience, University of Texas at Dallas, Richardson, TX, USA. FAU - Bontala, Sruti AU - Bontala S AD - Department of Molecular Biomedical Sciences, NC State University College of Veterinary Medicine, Raleigh, NC, USA. FAU - Deep, Ojasvi AU - Deep O AD - Department of Molecular Biomedical Sciences, NC State University College of Veterinary Medicine, Raleigh, NC, USA. FAU - Casey, Jacob AU - Casey J AD - Department of Molecular Biomedical Sciences, NC State University College of Veterinary Medicine, Raleigh, NC, USA. FAU - Kubofcik, Ramsey AU - Kubofcik R AUID- ORCID: 0009-0004-6494-3734 AD - Kimberly and Eric J. Waldman Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, NY, USA. AD - Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. AD - Marc and Jennifer Lipschultz Precision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. AD - Mark Lebwohl Center for Neuroinflammation and Sensation, Icahn School of Medicine at Mount Sinai, New York, NY, USA. AD - Allen Discovery Center for Neuroimmune Interactions, Icahn School of Medicine at Mount Sinai, New York, NY, USA. FAU - Schaffer, Daniel L AU - Schaffer DL AD - Kimberly and Eric J. Waldman Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, NY, USA. AD - Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. AD - Marc and Jennifer Lipschultz Precision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. AD - Mark Lebwohl Center for Neuroinflammation and Sensation, Icahn School of Medicine at Mount Sinai, New York, NY, USA. AD - Allen Discovery Center for Neuroimmune Interactions, Icahn School of Medicine at Mount Sinai, New York, NY, USA. FAU - Melhem, Hassan AU - Melhem H AD - Department of Biomedicine, University of Basel, Basel, Switzerland. FAU - Keyes, Gregory S AU - Keyes GS AD - Lipid Peroxidation Unit, Laboratory of Clinical Investigation, National Institute on Aging, National Institutes of Health, Baltimore, MD, USA. FAU - Davis, John M AU - Davis JM AD - Department of Psychiatry, Psychiatry College of Medicine; University of Illinois at Chicago, Chicago, IL, USA. FAU - Niess, Jan Hendrik AU - Niess JH AUID- ORCID: 0000-0001-6902-5650 AD - Department of Biomedicine, University of Basel, Basel, Switzerland. FAU - Price, Theodore J AU - Price TJ AUID- ORCID: 0000-0002-6971-6221 AD - Center for Advanced Pain Studies and Department of Neuroscience, University of Texas at Dallas, Richardson, TX, USA. FAU - Ramsden, Christopher E AU - Ramsden CE AD - Lipid Peroxidation Unit, Laboratory of Clinical Investigation, National Institute on Aging, National Institutes of Health, Baltimore, MD, USA. AD - Intramaural Program of the National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Baltimore, MD, USA. FAU - Kim, Brian S AU - Kim BS AUID- ORCID: 0000-0002-8100-7161 AD - Kimberly and Eric J. Waldman Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, NY, USA. AD - Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. AD - Marc and Jennifer Lipschultz Precision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. AD - Mark Lebwohl Center for Neuroinflammation and Sensation, Icahn School of Medicine at Mount Sinai, New York, NY, USA. AD - Allen Discovery Center for Neuroimmune Interactions, Icahn School of Medicine at Mount Sinai, New York, NY, USA. AD - Laboratory of Neuroimmunology, Institute of Bioelectronic Medicine, Feinstein Institute for Medical Research, Northwell Health, NY, USA. FAU - Alles, Sascha R A AU - Alles SRA AUID- ORCID: 0000-0001-8532-8950 AD - Department of Anesthesia, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA. FAU - Mishra, Santosh K AU - Mishra SK AUID- ORCID: 0000-0003-1149-3480 AD - Department of Molecular Biomedical Sciences, NC State University College of Veterinary Medicine, Raleigh, NC, USA. skmishra@ncsu.edu. AD - Comparative Medicine Institute; NC State University, Raleigh, NC, USA. skmishra@ncsu.edu. AD - Genetics and Genomics Academy, NC State University, Raleigh, NC, USA. skmishra@ncsu.edu. AD - Comparative Pain Research and Education Center, NC State University, Raleigh, NC, USA. skmishra@ncsu.edu. LA - eng GR - AR077692/U.S. Department of Health & Human Services | NIH | National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)/ PT - Journal Article DEP - 20261029 PL - England TA - Commun Biol JT - Communications biology JID - 101719179 RN - 0 (Receptors, G-Protein-Coupled) RN - 0 (GPR35 protein, mouse) RN - 0 (Dietary Fats) SB - IM MH - Animals MH - *Receptors, G-Protein-Coupled/metabolism/genetics MH - *Pruritus/metabolism/etiology MH - Mice MH - *Dietary Fats/metabolism/adverse effects MH - *Sensory Receptor Cells/metabolism MH - Mice, Inbred C57BL MH - Signal Transduction MH - Male MH - Disease Models, Animal MH - Mice, Knockout COIS- Competing interests: S.K.M. filed an invention disclosure at Office of Technology Licensing at North Carolina State University. NCSU Ref #2026-023 titled "Therapeutic Potential of GPR35 Blockers in inflammation and itch". Inventors: S.K.M. & B.S.K.; IP filing not applicable. All other authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:28 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/10 00:00 [received] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/30 00:28 [entrez] AID - 10.1038/s42003-026-11087-x [pii] AID - 10.1038/s42003-026-11087-x [doi] PST - epublish SO - Commun Biol. 2026 Oct 29;9(1):1393. doi: 10.1038/s42003-026-11087-x. PMID- 42957843 OWN - NLM STAT- Publisher LR - 20261030 IS - 1876-7931 (Electronic) IS - 1876-7931 (Linking) DP - 2026 Oct 29 TI - Pulse wave velocity measurement assessing the superficial carotid-femoral distance via smartphone-based light detection and ranging. LID - 10.1007/s40477-026-01252-7 [doi] AB - PURPOSE: Carotid-femoral pulse wave velocity (cfPWV) is the gold standard for non-invasive assessment of aortic stiffness. Tape measurement of the carotid-femoral distance typically requires an assistant to hold the tape while the operator maintains the Doller transducer position. This study assessed the concordance and intra-observer reliability of distance measurements obtained via smartphone-based light detection and ranging (LiDAR) technology compared with traditional tape measurement. METHODS: A cohort of 46 adult participants (mean age, 50.0+/-12.7 years) underwent Doppler ultrasound evaluation to determine carotid and femoral pulse transit times. The operator measured the superficial carotid-femoral distance using tape and smartphone LiDAR in alternating sequences. Duplicate measurements were taken following a washout period to evaluate intra-observer reliability. RESULTS: LiDAR distance measurements strongly agreed with tape measurements (ICC (2,1) = 0.92, 95% CI 0.81-0.97; mean bias <0.01 m). The resulting cfPWV calculations demonstrated good agreement (ICC (2,1) = 0.94, 95% CI 0.86-0.98; mean bias, 0.03 m/s). Both modalities exhibited high intra-observer reliability (tape ICC (3,1) = 0.98; LiDAR ICC (3,1) = 0.99). The diagnostic classification for elevated cfPWV (> 10 m/s) showed near-perfect concordance between the methods (kappa > 0.98). CONCLUSIONS: Smartphone-based LiDAR telemetry provides distance measurements and cfPWV estimates that are highly concordant with traditional tape measurements, offering a reliable single-operator alternative for superficial distance acquisition. While these findings confirm technical equivalence and intra-observer reliability, further studies incorporating reference standards and quantitative time-motion analyses are required to confirm the clinical workflow advantages of this method. CI - (c) 2026. Societa Italiana di Ultrasonologia in Medicina e Biologia (SIUMB). FAU - Posokhov, Igor N AU - Posokhov IN AUID- ORCID: 0000-0002-2381-0351 AD - West Kazakhstan Marat Ospanov Medical University, Maresyev Str., 68, 030000, Aktobe, Kazakhstan. igor@posohov.ru. LA - eng PT - Journal Article DEP - 20261029 PL - Italy TA - J Ultrasound JT - Journal of ultrasound JID - 101315005 SB - IM OTO - NOTNLM OT - Aortic stiffness OT - Carotid-femoral pulse wave velocity OT - LiDAR OT - Reliability OT - Smartphone OT - Vascular ultrasound COIS- Declarations. Conflict of interest: The authors have not disclosed any competing interests. Ethical approval: This is an observational study. The West Kazakhstan Marat Ospanov Medical University Research Ethics Committee has confirmed that no ethical approval is required. Informed consent was obtained from all individual participants included in the study. The author affirms that human research participants provided informed consent for publication of the images in Figure 1. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:28 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/07/23 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/30 00:28 [entrez] AID - 10.1007/s40477-026-01252-7 [pii] AID - 10.1007/s40477-026-01252-7 [doi] PST - aheadofprint SO - J Ultrasound. 2026 Oct 29. doi: 10.1007/s40477-026-01252-7. PMID- 42957839 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2399-3642 (Electronic) IS - 2399-3642 (Linking) VI - 9 IP - 1 DP - 2026 Oct 29 TI - Towards a sequential microbiome therapy for HPV-associated cervicovaginal dysbiosis. LID - 1422 [pii] LID - 10.1038/s42003-026-11208-6 [doi] AB - Persistent high-risk human papillomavirus (hrHPV) infections are the principal cause of cervical cancer, and are increasingly associated with ecological instability of the cervicovaginal microbiome. Multiomics studies indicate that hrHPV persistence is linked to reduced Lactobacillus dominance, increased anaerobic taxa, and metabolic alterations that may compromise epithelial integrity and antiviral immunity. In this Perspective article, we introduce a sequential microbiome therapy (SMT) framework designed to restore cervicovaginal ecological balance and support viral clearance. SMT is a two-phase, strain-resolved strategy. The first phase targets dysbiotic communities using antimicrobials, biofilm-disrupting or microbiota-modulating approaches to create a window for ecological reset. The second phase introduces health-associated Lactobacillus species selected for their acidification capacity, competitive fitness, and ability to stabilize the mucosal environment. We outline the ecological principles underpinning SMT and discuss its development as a potentially scalable microbiome-directed strategy for restoring cervicovaginal health and managing hrHPV-associated disease within existing cervical cancer prevention frameworks. CI - (c) 2026. The Author(s). FAU - Molina, Mariano A AU - Molina MA AUID- ORCID: 0000-0003-4260-0509 AD - Department of Pathology, Vrije Universiteit Amsterdam, Amsterdam UMC location VUmc, Amsterdam, The Netherlands. mariano.molina.beitia@ki.se. AD - Cancer Center Amsterdam, Imaging and Biomarkers, Amsterdam, The Netherlands. mariano.molina.beitia@ki.se. AD - Department of Laboratory Medicine, Karolinska Institutet, ANA Futura, Stockholm, Sweden. mariano.molina.beitia@ki.se. FAU - Zhang, Yichan AU - Zhang Y AD - Centre for Translational Microbiome Research (CTMR), Department of Microbiology, Tumor, and Cell Biology, Karolinska Institutet, Stockholm, Sweden. FAU - Steenbergen, Renske D M AU - Steenbergen RDM AUID- ORCID: 0000-0002-2327-9839 AD - Department of Pathology, Vrije Universiteit Amsterdam, Amsterdam UMC location VUmc, Amsterdam, The Netherlands. AD - Cancer Center Amsterdam, Imaging and Biomarkers, Amsterdam, The Netherlands. FAU - Melchers, Willem J G AU - Melchers WJG AD - Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands. FAU - Du, Juan AU - Du J AUID- ORCID: 0000-0001-7649-9571 AD - Centre for Translational Microbiome Research (CTMR), Department of Microbiology, Tumor, and Cell Biology, Karolinska Institutet, Stockholm, Sweden. juan.du@ki.se. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - Commun Biol JT - Communications biology JID - 101719179 SB - IM MH - Female MH - Humans MH - *Dysbiosis/therapy/microbiology MH - *Microbiota MH - *Papillomavirus Infections/therapy/microbiology/virology/complications MH - *Human Papillomavirus Viruses MH - *Vagina/microbiology/virology MH - *Cervix Uteri/microbiology/virology MH - Uterine Cervical Neoplasms/prevention & control/virology COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:28 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/12/23 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/30 00:28 [entrez] AID - 10.1038/s42003-026-11208-6 [pii] AID - 10.1038/s42003-026-11208-6 [doi] PST - epublish SO - Commun Biol. 2026 Oct 29;9(1):1422. doi: 10.1038/s42003-026-11208-6. PMID- 42957834 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1573-2959 (Electronic) IS - 0167-6369 (Linking) VI - 198 IP - 11 DP - 2026 Oct 30 TI - Gap filling in long-term AOD time series using Iterative Singular Spectrum Analysis at a tropical AERONET site. LID - 1265 [pii] LID - 10.1007/s10661-026-16067-6 [doi] AB - Aerosol optical depth (AOD) is widely used in studies related to atmospheric monitoring, climate variability, and air quality, with the Aerosol RObotic NETwork (AERONET) providing high-quality records. However, AERONET AOD time series frequently contain observational gaps associated with cloud cover, especially in tropical regions characterized by strong hydrological seasonality. In this study, the performance of Iterative Singular Spectrum Analysis (ISSA) was evaluated for gap-filling an incomplete AOD time series from the AERONET Cuiaba-Miranda station (AOD-AERONET) in the Central-West region of Brazil. Daily AOD-AERONET data between 2005 and 2024 were used, with approximately 47% of the daily observations identified as gaps and showing a predominantly seasonal distribution. The application of ISSA to the gap-filling of the AOD series involved the evaluation of different initialization and cross-validation strategies using model performance metrics, as well as different reconstruction parameters. The results showed that the climatological initialization, based on the Day of the Year (DOY) mean, outperformed the global-mean initialization, producing smaller errors and greater temporal consistency of the reconstruction, and that the cross-validation design plays an important role in the selection of optimal parameters. CI - (c) 2026. The Author(s). FAU - Schmidt, Tiago AU - Schmidt T AUID- ORCID: 0009-0001-9323-5495 AD - Department of Teaching, Federal Institute of Education, Science and Technology of Mato Grosso, Rua das Avencas, Sinop, 78550-178, Mato Grosso, Brazil. tiagaots@gmail.com. AD - Graduate Program in Environmental Physics, Federal University of Mato Grosso, Avenida Fernando Correa da Costa, Cuiaba, 78060-900, Mato Grosso, Brazil. tiagaots@gmail.com. FAU - da Silva Palacios, Rafael AU - da Silva Palacios R AUID- ORCID: 0000-0002-7628-1342 AD - Graduate Program in Environmental Physics, Federal University of Mato Grosso, Avenida Fernando Correa da Costa, Cuiaba, 78060-900, Mato Grosso, Brazil. AD - Institute of Geosciences, Federal University of Para, Rua Augusto Correa, Belem, 66075-110, Para, Brazil. FAU - de Souza, Amaury AU - de Souza A AUID- ORCID: 0000-0001-8168-1482 AD - Physics Department, Federal University of Mato Grosso do Sul, Avenida Costa e Silva, Campo Grande, 79070-900, Mato Grosso do Sul, Brazil. FAU - Curado, Leone Francisco Amorim AU - Curado LFA AUID- ORCID: 0000-0002-0375-7725 AD - Graduate Program in Environmental Physics, Federal University of Mato Grosso, Avenida Fernando Correa da Costa, Cuiaba, 78060-900, Mato Grosso, Brazil. LA - eng PT - Journal Article DEP - 20261030 PL - Netherlands TA - Environ Monit Assess JT - Environmental monitoring and assessment JID - 8508350 RN - 0 (Aerosols) RN - 0 (Air Pollutants) SB - IM MH - *Environmental Monitoring/methods MH - Aerosols/analysis MH - *Air Pollutants/analysis MH - Brazil MH - Tropical Climate MH - Air Pollution/statistics & numerical data MH - Spectrum Analysis MH - Seasons OTO - NOTNLM OT - AERONET OT - Aerosol optical depth OT - Gap filling OT - Singular Spectrum Analysis COIS- Declarations. Ethical approval: All authors have read, understood, and have complied as applicable with the statement on "Ethical responsibilities of Authors" as found in the Instructions for Authors. Conflict of interest: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:28 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/07/01 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/30 00:28 [entrez] AID - 10.1007/s10661-026-16067-6 [pii] AID - 10.1007/s10661-026-16067-6 [doi] PST - epublish SO - Environ Monit Assess. 2026 Oct 30;198(11):1265. doi: 10.1007/s10661-026-16067-6. PMID- 42957826 OWN - NLM STAT- Publisher LR - 20261030 IS - 1476-5578 (Electronic) IS - 1359-4184 (Linking) DP - 2026 Oct 28 TI - Evolutionary neuropharmacology of serotonergic psychedelics: a cross-species perspective on mechanisms, consciousness, and therapeutic potential. LID - 10.1038/s41380-026-03939-z [doi] AB - Serotonergic psychedelics have revitalized psychiatric research by producing rapid and potentially sustained clinical benefits in selected neuropsychiatric conditions. However, the mechanisms linking receptor activation to neural plasticity, altered conscious experience, and clinical improvement remain incompletely understood, while the evolutionary origins of psychedelic signaling and the translational value of animal models continue to be actively debated. In this review, we integrate evolutionary biology, molecular pharmacology, comparative neuroscience, and computational psychiatry into a unified framework for understanding psychedelic action across biological scales. Building upon predictive processing and the Relaxed Beliefs Under Psychedelics (REBUS) framework, we discuss how serotonergic psychedelics transiently reshape hierarchical inference, providing computational principles that can be experimentally investigated across species despite profound differences in neural architecture and subjective experience. Comparative analyses from invertebrates to non-human primates further suggest that conserved receptor pharmacology coexists with species-specific circuit organization, highlighting that animal models should be interpreted as complementary platforms for investigating molecular, cellular, and computational mechanisms rather than direct proxies for human hallucinatory experiences. We further discuss preclinical and emerging translational evidence suggesting that biased receptor signaling and non-hallucinogenic psychoplastogens may partially dissociate plasticity-related therapeutic actions from hallucinogenic effects, providing new opportunities for mechanism-based drug development. By integrating evolutionary biology with molecular psychiatry, this review provides a mechanistic framework linking receptor pharmacology, neural plasticity, circuit computation, and therapeutic outcomes, thereby offering new conceptual and experimental strategies for developing next-generation psychedelic therapeutics. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature Limited. FAU - Zhai, Haojiang AU - Zhai H AD - Interdisciplinary Laboratory for Frontier Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China. AD - School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, Anhui, 230026, China. FAU - Wang, Hongshuang AU - Wang H AD - Interdisciplinary Laboratory for Frontier Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China. FAU - Wang, Yibo AU - Wang Y AD - Interdisciplinary Laboratory for Frontier Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China. yibo.wang@ciac.ac.cn. FAU - Wang, Xiaohui AU - Wang X AUID- ORCID: 0000-0002-3415-5612 AD - Interdisciplinary Laboratory for Frontier Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China. xiaohui.wang@ciac.ac.cn. AD - School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, Anhui, 230026, China. xiaohui.wang@ciac.ac.cn. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - Mol Psychiatry JT - Molecular psychiatry JID - 9607835 SB - IM COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:27 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/11/20 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/09/16 00:00 [revised] PHST- 2026/10/30 00:27 [entrez] AID - 10.1038/s41380-026-03939-z [pii] AID - 10.1038/s41380-026-03939-z [doi] PST - aheadofprint SO - Mol Psychiatry. 2026 Oct 28. doi: 10.1038/s41380-026-03939-z. PMID- 42957824 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2364-8961 (Electronic) IS - 2364-8961 (Linking) VI - 384 IP - 4 DP - 2026 Oct 30 TI - Macrocycle-Integrated MOF Nanoarchitectures: Cooperative Active Sites from Pillararenes, Calixarenes, and Crown Ethers. LID - 39 [pii] LID - 10.1007/s41061-026-00572-w [doi] AB - Metal-organic frameworks (MOFs) offer crystalline porosity, modular structures, and tunable chemical environments, but they often lack the molecular recognition needed for highly selective adsorption, transport, and catalysis. Integrating macrocycles such as pillararenes, calixarenes, and crown ethers provides a route to macrocycle@MOF nanoarchitectures in which supramolecular cavities cooperate with framework pores, metal nodes, and linker functionalities. Calixarene-based MOFs create adaptive pockets for iodine capture, NO(2) sensing, and molecular separation; pillararene systems provide rigid, electron-rich channels for sieving, controlled release, and supramolecular docking; and crown-ether-functionalized MOFs enable selective ion transport, lithium sieving, and Sr(2+)/Cs(+) remediation. Their function arises from coupled host-guest recognition, pore confinement, electrostatic complementarity, and active-site organization. Key challenges include pore blocking, synthetic complexity, structural disorder, incomplete active-site validation, and device integration. This review highlights design principles for converting macrocycle-integrated MOFs from proof-of-concept materials into practical recognition platforms for advanced catalysis, separations, sensing, remediation, energy technologies, and biomedical applications. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature Switzerland AG. FAU - Mohan, Brij AU - Mohan B AD - Centro de Quimica Estrutural, Institute of Molecular Sciences, Instituto Superior Tecnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisbon, Portugal. brij.mohan@tecnico.ulisboa.pt. FAU - Sharma, Harish Kumar AU - Sharma HK AD - Department of Chemistry, Kurukshetra University, Kurukshetra, 136119, India. FAU - Najafov, Bakhtiyar AU - Najafov B AD - Materials Engineering, RWTH Aachen University, Templergraben 55, 52062, Aachen, Germany. AD - New Supramolecular Compounds Laboratory, Gas and Chemistry, Research Institute of Geotechnological Problems of Oil, Azerbaijan State Oil and Industry University, 227 Dilara Aliyeva Street, Baku, AZ1010, Azerbaijan. FAU - Garazade, Ismayil M AU - Garazade IM AD - Centro de Quimica Estrutural, Institute of Molecular Sciences, Instituto Superior Tecnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisbon, Portugal. AD - Faculty of Chemistry, Baku State University, Baku, 1148, Azerbaijan. FAU - Seyidova, Khayala AU - Seyidova K AD - Institute of Petrochemical Processes Named After Academician Yusif Mammadaliyev, Ministry of Science and Education of the Republic of Azerbaijan, Khojaly Ave. 30, AZ1025, Baku, Azerbaijan. FAU - Gupta, Rakesh Kumar AU - Gupta RK AD - Key Lab of Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, State Key Laboratory of Crystal Materials, Shandong University, Jinan, 250100, Shandong, China. FAU - Virender, Virender AU - Virender V AD - Centro de Quimica Estrutural, Institute of Molecular Sciences, Instituto Superior Tecnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisbon, Portugal. FAU - da Silva, M Fatima C Guedes AU - da Silva MFCG AD - Centro de Quimica Estrutural, Institute of Molecular Sciences, Instituto Superior Tecnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisbon, Portugal. FAU - Pombeiro, Armando J L AU - Pombeiro AJL AD - Centro de Quimica Estrutural, Institute of Molecular Sciences, Instituto Superior Tecnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisbon, Portugal. FAU - Kim, Sang Sub AU - Kim SS AD - Department of Materials Science and Engineering, Inha University, 100 Inha-Ro, Incheon, 22212, Republic of Korea. sangsub@inha.ac.kr. LA - eng GR - UID/00100/2025 (https://doi.org/10.54499/UID/00100/2025), UID/PRR/00100/2025 (https://doi.org/10.54499/UID/PRR/00100/2025), and UID/PRR2/00100/2025 (https://doi.org/10.54499/UID/PRR2/00100/2025)/Fundacao para a Ciencia e a Tecnologia (FCT), Portugal/ PT - Journal Article PT - Review DEP - 20261030 PL - Switzerland TA - Top Curr Chem (Cham) JT - Topics in current chemistry (Cham) JID - 101691301 RN - 130036-26-9 (Calixarenes) RN - 0 (Crown Ethers) RN - 0 (Macrocyclic Compounds) SB - IM MH - *Calixarenes/chemistry MH - *Crown Ethers/chemistry MH - *Macrocyclic Compounds/chemistry MH - *Nanostructures/chemistry MH - Catalytic Domain OTO - NOTNLM OT - Calixarenes OT - Crown ethers OT - MOFs nanoarchitectures OT - Pillararenes OT - Reactivity and functionality COIS- Declarations. Conflict of interest: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:27 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/08/13 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/30 00:27 [entrez] AID - 10.1007/s41061-026-00572-w [pii] AID - 10.1007/s41061-026-00572-w [doi] PST - epublish SO - Top Curr Chem (Cham). 2026 Oct 30;384(4):39. doi: 10.1007/s41061-026-00572-w. PMID- 42957811 OWN - NLM STAT- Publisher LR - 20261030 IS - 1618-2650 (Electronic) IS - 1618-2642 (Linking) DP - 2026 Oct 30 TI - A micro-sampling strategy for monitoring plastic degradation via volatile organic profiling using headspace-solid-phase microextraction GC-MS. Application to historical cellulose acetate negatives. LID - 10.1007/s00216-026-06879-3 [doi] AB - The historical, symbolic, and material value of cultural heritage objects imposes strict limitations on sampling, requiring analytical strategies capable of examining minute samples with minimal or no destruction. While non-invasive and minimally invasive methods are well established for inorganic materials, the characterization of organic components remains challenging. Spectroscopic techniques have partly addressed this issue, but they are often limited by spectral complexity and matrix interferences. Sample preparation methods typically required prior to chromatographic analyses usually consume a significant portion of the available sample, hindering the application of complementary analytical techniques. Here, a micro-sampling approach coupled with headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS) was adapted and evaluated for the first time. The methodology enables sequential analytical workflows in which the same micro-sample can be further analyzed by complementary techniques. To demonstrate its applicability, naturally aged cellulose acetate negatives from the National Historical Museum of Argentina (MHN) were investigated. An untargeted analytical strategy was used to comprehensively characterize volatile organic compound (VOC) emissions and correlate them with degradation stage and other material features. A total of 40 VOCs were consistently detected across all fiber coatings, providing a robust volatile signature for material characterization and degradation assessment. The PDMS fiber discriminated against degradation stages, whereas the PDMS/DVB fiber differentiated samples according to format (small vs. large negatives), suggesting that VOC profiles may also reflect differences in manufacturing processes, use history, or conservation treatments. These results demonstrate the potential of HS-SPME-GC-MS for the non-destructive characterization of heritage plastics and degradation assessment. Moreover, the proposed micro-sampling strategy is fully compatible with complementary analytical workflows, including cross-sectional and micro-spectroscopic analyses, providing a methodological framework for preventive conservation based on volatile degradation markers. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature. FAU - Buongarzoni, Laura Antonella AU - Buongarzoni LA AD - Centro de Estudios sobre Patrimonios y Ambiente (CEPyA, Escuela de Arte y Patrimonio-Escuela de Habitat y Sostenibilidad), Universidad Nacional de San Martin (UNSAM), Av. 25 de Mayo y Francia, Campus Miguelete, Buenos Aires, Argentina. AD - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires, Argentina. FAU - Lopez, Rocio AU - Lopez R AD - Centro de Estudios sobre Patrimonios y Ambiente (CEPyA, Escuela de Arte y Patrimonio-Escuela de Habitat y Sostenibilidad), Universidad Nacional de San Martin (UNSAM), Av. 25 de Mayo y Francia, Campus Miguelete, Buenos Aires, Argentina. AD - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires, Argentina. FAU - Morales, Ana AU - Morales A AD - Centro de Estudios sobre Patrimonios y Ambiente (CEPyA, Escuela de Arte y Patrimonio-Escuela de Habitat y Sostenibilidad), Universidad Nacional de San Martin (UNSAM), Av. 25 de Mayo y Francia, Campus Miguelete, Buenos Aires, Argentina. AD - Centro Tarea, Escuela de Arte y Patrimonio, Universidad Nacional de San Martin (UNSAM), Benito Quinquela Martin 1784, Ciudad Autonoma de Buenos Aires, Argentina. FAU - Marte, Fernando AU - Marte F AD - Centro de Estudios sobre Patrimonios y Ambiente (CEPyA, Escuela de Arte y Patrimonio-Escuela de Habitat y Sostenibilidad), Universidad Nacional de San Martin (UNSAM), Av. 25 de Mayo y Francia, Campus Miguelete, Buenos Aires, Argentina. FAU - Castells, Cecilia AU - Castells C AD - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires, Argentina. AD - Laboratorio de Investigacion y Desarrollo de Metodos Analiticos, LIDMA, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 47 and 115 (B1900AJL), La Plata, Argentina. FAU - Tascon, Marcos AU - Tascon M AD - Centro de Estudios sobre Patrimonios y Ambiente (CEPyA, Escuela de Arte y Patrimonio-Escuela de Habitat y Sostenibilidad), Universidad Nacional de San Martin (UNSAM), Av. 25 de Mayo y Francia, Campus Miguelete, Buenos Aires, Argentina. mtascon@unsam.edu.ar. AD - Instituto de Investigacion e Ingenieria Ambiental (IIIA, CONICET-UNSAM), Av. 25 de Mayo y Francia, Campus Miguelete, Buenos Aires, Argentina. mtascon@unsam.edu.ar. LA - eng GR - 80020250100125SM/Universidad Nacional de San Martin/ GR - PICT 2021-0200/Agencia Nacional de Promocion Cientifica y Tecnologica/ PT - Journal Article DEP - 20261030 PL - Germany TA - Anal Bioanal Chem JT - Analytical and bioanalytical chemistry JID - 101134327 SB - IM OTO - NOTNLM OT - Cellulose acetate OT - HS-SPME-GC-MS OT - Nondestructive analysis OT - Volatile degradome COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:26 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/08/06 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/30 00:26 [entrez] AID - 10.1007/s00216-026-06879-3 [pii] AID - 10.1007/s00216-026-06879-3 [doi] PST - aheadofprint SO - Anal Bioanal Chem. 2026 Oct 30. doi: 10.1007/s00216-026-06879-3. PMID- 42957806 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 2399-3669 (Electronic) IS - 2399-3669 (Linking) VI - 9 IP - 1 DP - 2026 Oct 28 TI - Intermediate spillover-enabled cooperation in single-atom catalysts. LID - 338 [pii] LID - 10.1038/s42004-026-02158-5 [doi] AB - The linear scaling relationships between multiple intermediates constrain catalytic performance, especially in single-atom catalysts (SACs) with isolated sites. This review focuses on overcoming this limitation by constructing SACs-based cooperative systems that exploit the directional spillover of key intermediates between functionally distinct sites. We systematically summarize how hydrogen-, oxygen-, carbon-, and nitrogen intermediates spillover between four representative cooperation paradigms, including multi-center SACs, SACs-nanoparticle hybrids, SACs-support interaction, and single-atom alloys. Through functional decoupling and site-to-site relay, these strategies reduce kinetic barriers of rate-determining steps and reconstruct reaction pathways. Beyond mitigating activity-selectivity-stability trade-offs of SACs, intermediate spillover mechanism moves catalyst design from trial-and-error approaches toward mechanism-guided engineering. CI - (c) 2026. The Author(s). FAU - Shan, Minqi AU - Shan M AD - Faculty of Maritime and Transportation, Ningbo University, Ningbo, China. FAU - Yuan, Jinliang AU - Yuan J AD - Faculty of Maritime and Transportation, Ningbo University, Ningbo, China. yuanjinliang@nbu.edu.cn. FAU - Zhang, Tianyu AU - Zhang T AUID- ORCID: 0000-0002-1250-9395 AD - Faculty of Maritime and Transportation, Ningbo University, Ningbo, China. zhangtianyu@nbu.edu.cn. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - Commun Chem JT - Communications chemistry JID - 101725670 COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:26 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/11 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/30 00:26 [entrez] AID - 10.1038/s42004-026-02158-5 [pii] AID - 10.1038/s42004-026-02158-5 [doi] PST - epublish SO - Commun Chem. 2026 Oct 28;9(1):338. doi: 10.1038/s42004-026-02158-5. PMID- 42957792 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 1432-1262 (Electronic) IS - 0179-1958 (Linking) VI - 41 IP - 1 DP - 2026 Aug 4 TI - Robotic surgery in inflammatory bowel disease: a systematic review. LID - 174 [pii] LID - 10.1007/s00384-026-05213-8 [doi] AB - BACKGROUND: Surgical treatment remains an essential component in the management of inflammatory bowel disease (IBD) despite advances in medical therapy. Although minimally invasive surgery is preferred, technical challenges related to chronic inflammation and altered anatomy may limit laparoscopic approaches. Robotic surgery has been increasingly adopted, but its role in IBD surgery remains to be clearly defined. OBJECTIVES: To synthesize the available evidence on the feasibility, safety, and perioperative outcomes of robotic surgery in patients with IBD. METHODS: A systematic review was conducted according to PRISMA guidelines. Studies were identified through an electronic search of the PubMed database for reports evaluating outcomes of robotic surgery in IBD. Eligible studies included case series, comparative studies, and systematic reviews published in English. Primary outcomes were conversion to open surgery and postoperative complications; secondary outcomes included operative time, length of stay (LOS), reoperation, readmission, and anastomotic leak. RESULTS: Thirty-four studies were included. Robotic surgery was consistently associated with longer operative time. However, postoperative morbidity, major complications, anastomotic leak, reoperation, and mortality rates were comparable between techniques. Robotic approaches demonstrated lower and favorable conversion rates, particularly in complex inflammatory phenotypes or pelvic procedures. Length of stay and estimated blood loss were similar or numerically lower in several robotic cohorts. CONCLUSION: Robotic surgery is a safe and feasible minimally invasive approach for IBD patients, with perioperative outcomes comparable to conventional surgery. Its potential advantages may be most relevant in complex or technically demanding cases, although high-quality prospective studies are needed to better define its role. CI - (c) 2026. The Author(s). FAU - Todescatto, Alexandra Damasio AU - Todescatto AD AUID- ORCID: 0000-0001-7273-3076 AD - IBD Outpatient Clinics, Pontificia Universidade Catolica Do Parana (PUCPR), Curitiba, Brazil. FAU - Mendes, Carlos Ramon Silveira AU - Mendes CRS AUID- ORCID: 0000-0002-1204-6722 AD - Hospital Santa Izabel, Salvador, Bahia, Brazil. FAU - Araujo, Sergio Eduardo Alonso AU - Araujo SEA AUID- ORCID: 0000-0002-2361-254X AD - Einstein Hospital Israelita, Sao Paulo, Brazil. sergio.araujo@einstein.br. FAU - Kotze, Paulo Gustavo AU - Kotze PG AUID- ORCID: 0000-0002-2053-5315 AD - IBD Outpatient Clinics, Pontificia Universidade Catolica Do Parana (PUCPR), Curitiba, Brazil. LA - eng PT - Journal Article PT - Review PT - Systematic Review DEP - 20260804 PL - Germany TA - Int J Colorectal Dis JT - International journal of colorectal disease JID - 8607899 SB - IM MH - Humans MH - *Robotic Surgical Procedures/adverse effects MH - *Inflammatory Bowel Diseases/surgery MH - Treatment Outcome MH - Operative Time MH - Postoperative Complications/etiology MH - Length of Stay OTO - NOTNLM OT - Colorectal surgery OT - Crohn's disease OT - Inflammatory bowel disease OT - Minimally invasive surgery OT - Robotic surgery OT - Ulcerative colitis COIS- Declarations. Ethics approval: This study is a systematic review. Ethical approval was not required. Consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare that they have no competing interests to disclose. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:26 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/27 00:00 [received] PHST- 2026/07/21 00:00 [accepted] PHST- 2026/10/30 00:26 [entrez] AID - 10.1007/s00384-026-05213-8 [pii] AID - 10.1007/s00384-026-05213-8 [doi] PST - epublish SO - Int J Colorectal Dis. 2026 Aug 4;41(1):174. doi: 10.1007/s00384-026-05213-8. PMID- 42957784 OWN - NLM STAT- Publisher LR - 20261030 IS - 1476-4679 (Electronic) IS - 1465-7392 (Linking) DP - 2026 Oct 28 TI - Author Correction: Spatiotemporal coordination of actin regulators generates invasive protrusions in cell-cell fusion. LID - 10.1038/s41556-026-02144-y [doi] FAU - Lu, Yue AU - Lu Y AUID- ORCID: 0000-0002-3999-7050 AD - Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA. FAU - Walji, Tezin AU - Walji T AD - Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA. FAU - Ravaux, Benjamin AU - Ravaux B AD - Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA. FAU - Pandey, Pratima AU - Pandey P AD - Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA. FAU - Yang, Changsong AU - Yang C AD - Department of Biology, University of Pennsylvania, Philadelphia, PA, USA. FAU - Li, Bing AU - Li B AD - Department of Physiology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA. FAU - Luvsanjav, Delgermaa AU - Luvsanjav D AD - Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD, USA. FAU - Lam, Kevin H AU - Lam KH AD - Department of Bioengineering, University of Texas at Dallas, Richardson, TX, USA. FAU - Zhang, Ruihui AU - Zhang R AUID- ORCID: 0000-0003-1010-7242 AD - Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA. FAU - Luo, Zhou AU - Luo Z AD - Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA. FAU - Zhou, Chuanli AU - Zhou C AUID- ORCID: 0000-0003-1848-2055 AD - Department of Immunology, University of Texas Southwestern Medical Center, Dallas, TX, USA. FAU - Habela, Christa W AU - Habela CW AD - Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, USA. FAU - Snapper, Scott B AU - Snapper SB AUID- ORCID: 0000-0003-0267-4247 AD - Department of Pediatrics, Boston Children's Hospital, Boston, MA, USA. FAU - Li, Rong AU - Li R AUID- ORCID: 0000-0002-0540-6566 AD - Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA. AD - Mechanobiology Institute, National University of Singapore, Singapore, Singapore. FAU - Goldhamer, David J AU - Goldhamer DJ AUID- ORCID: 0000-0003-4605-8921 AD - Department of Molecular and Cell Biology, University of Connecticut Stem Cell Institute, Storrs, CT, USA. FAU - Schmidtke, David W AU - Schmidtke DW AD - Department of Bioengineering, University of Texas at Dallas, Richardson, TX, USA. FAU - Pan, Duojia AU - Pan D AUID- ORCID: 0000-0003-2890-4645 AD - Department of Physiology, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, USA. FAU - Svitkina, Tatyana M AU - Svitkina TM AUID- ORCID: 0000-0002-8424-447X AD - Department of Biology, University of Pennsylvania, Philadelphia, PA, USA. FAU - Chen, Elizabeth H AU - Chen EH AUID- ORCID: 0000-0003-2707-6083 AD - Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA. Elizabeth.Chen@UTSouthwestern.edu. AD - Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA. Elizabeth.Chen@UTSouthwestern.edu. AD - Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX, USA. Elizabeth.Chen@UTSouthwestern.edu. AD - Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, Dallas, TX, USA. Elizabeth.Chen@UTSouthwestern.edu. LA - eng PT - Published Erratum DEP - 20261028 PL - England TA - Nat Cell Biol JT - Nature cell biology JID - 100890575 SB - IM EFR - Nat Cell Biol. 2024 Nov;26(11):1860-1877. doi: 10.1038/s41556-024-01541-5. PMID: 39487253 EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:25 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/30 00:25 [entrez] AID - 10.1038/s41556-026-02144-y [pii] AID - 10.1038/s41556-026-02144-y [doi] PST - aheadofprint SO - Nat Cell Biol. 2026 Oct 28. doi: 10.1038/s41556-026-02144-y. PMID- 42957783 OWN - NLM STAT- Publisher LR - 20261030 IS - 1476-4679 (Electronic) IS - 1465-7392 (Linking) DP - 2026 Oct 29 TI - Methylated eEF1A selectively decodes mRNA to shape cancer proteomes. LID - 10.1038/s41556-026-02085-6 [doi] AB - Although mRNA decoding efficiency influences protein synthesis, it remains unknown whether the translation elongation machinery harbours intrinsic mechanisms that selectively modulate codon decoding to shape proteomes. Here we show that METTL13-catalysed dimethylation of the elongation factor eEF1A at lysine 55 (eEF1AK55me2) enhances decoding of select codons, preferentially increasing pro-oncogenic protein synthesis to promote tumorigenesis and targeted therapy resistance. Proteomic profiling and single-molecule imaging reveal that eEF1AK55me2 increases decoding efficiency, particularly for under-represented codons with weak wobble-position base pairing. In cancer cells, METTL13 knockdown reduced translation of oncogenic mRNA enriched in eEF1AK55me2-dependent codons, including KRAS; synonymous recoding with eEF1AK55me2-independent codons reversed this effect. METTL13 loss inhibited the growth of KRAS(G12C) inhibitor (sotorasib)-resistant lung adenocarcinoma syngeneic tumours and patient-derived xenografts. In relapsed autochthonous KRAS(G12C)-driven lung adenocarcinoma, METTL13 ablation mitigated sotorasib resistance and significantly extended survival in vivo. Among patients receiving a KRAS(G12C) inhibitor, inferred METTL13 activity was associated with inferior clinical response. These findings identify an eEF1AK55me2-mediated mRNA decoding mechanism promoting selective oncogenic protein synthesis and suggest METTL13 targeting may counteract KRAS-inhibitor resistance. CI - (c) 2026. The Author(s). FAU - Liu, Shuo AU - Liu S AD - Department of Biology, Stanford University, Stanford, CA, USA. FAU - Cruz-Navarrete, F Aaron AU - Cruz-Navarrete FA AUID- ORCID: 0000-0002-5233-581X AD - Department of Structural Biology, St Jude Children's Research Hospital, Memphis, TN, USA. FAU - Hausmann, Simone AU - Hausmann S AUID- ORCID: 0000-0001-9577-4225 AD - Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. FAU - Szkop, Krzysztof J AU - Szkop KJ AD - Department of Oncology-Pathology, Science for Life Laboratories, Karolinska Institute, Stockholm, Sweden. FAU - Ikram, Sabeen AU - Ikram S AUID- ORCID: 0009-0002-1856-8393 AD - Department of Biology, Stanford University, Stanford, CA, USA. FAU - Brady, Ryan A AU - Brady RA AUID- ORCID: 0000-0002-0408-3224 AD - Department of Structural Biology, St Jude Children's Research Hospital, Memphis, TN, USA. FAU - Lu, Xiaoyin AU - Lu X AUID- ORCID: 0000-0002-9244-325X AD - Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. FAU - Dong, Rui AU - Dong R AUID- ORCID: 0000-0001-6782-1186 AD - Department of Biology, Stanford University, Stanford, CA, USA. FAU - Vu, Julia AU - Vu J AUID- ORCID: 0000-0001-6386-3979 AD - Department of Biology, Stanford University, Stanford, CA, USA. FAU - Flores, Natasha M AU - Flores NM AD - Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. FAU - Zahn, Emily AU - Zahn E AUID- ORCID: 0009-0009-0431-5820 AD - Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO, USA. FAU - Thompson, Julia AU - Thompson J AD - Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. FAU - Garcia, Ben A AU - Garcia BA AUID- ORCID: 0000-0003-3596-4750 AD - Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO, USA. FAU - Larsson, Ola AU - Larsson O AUID- ORCID: 0000-0003-1412-1308 AD - Department of Oncology-Pathology, Science for Life Laboratories, Karolinska Institute, Stockholm, Sweden. ola.larsson@ki.se. FAU - Topisirovic, Ivan AU - Topisirovic I AUID- ORCID: 0000-0002-5510-9762 AD - Department of Biochemistry, McGill University, Montreal, Quebec, Canada. ivan.topisirovic@mcgill.ca. FAU - Blanchard, Scott C AU - Blanchard SC AUID- ORCID: 0000-0003-2717-9365 AD - Department of Structural Biology, St Jude Children's Research Hospital, Memphis, TN, USA. Scott.Blanchard@STJUDE.ORG. AD - Department of Chemical Biology and Therapeutics, St Jude Children's Research Hospital, Memphis, TN, USA. Scott.Blanchard@STJUDE.ORG. FAU - Gozani, Or AU - Gozani O AUID- ORCID: 0000-0002-1365-4463 AD - Department of Biology, Stanford University, Stanford, CA, USA. ogozani@stanford.edu. FAU - Mazur, Pawel K AU - Mazur PK AUID- ORCID: 0000-0002-5820-8344 AD - Department of Experimental Radiation Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. pkmazur@mdanderson.org. LA - eng GR - R01 CA272844/U.S. Department of Health & Human Services | National Institutes of Health (NIH)/ GR - R01 CA278940/U.S. Department of Health & Human Services | National Institutes of Health (NIH)/ GR - R01 CA236949/U.S. Department of Health & Human Services | National Institutes of Health (NIH)/ GR - R01 CA266280/U.S. Department of Health & Human Services | National Institutes of Health (NIH)/ GR - R01 CA272843/U.S. Department of Health & Human Services | National Institutes of Health (NIH)/ GR - T32AG000266/U.S. Department of Health & Human Services | National Institutes of Health (NIH)/ GR - K99 CA255936/U.S. Department of Health & Human Services | National Institutes of Health (NIH)/ GR - R01 HD106051/U.S. Department of Health & Human Services | National Institutes of Health (NIH)/ GR - R01 AI118891/U.S. Department of Health & Human Services | National Institutes of Health (NIH)/ GR - R01GM079238/U.S. Department of Health & Human Services | National Institutes of Health (NIH)/ GR - R35 GM139569/U.S. Department of Health & Human Services | National Institutes of Health (NIH)/ GR - R01 CA272844/U.S. Department of Health & Human Services | National Institutes of Health (NIH)/ GR - R01 CA278940/U.S. Department of Health & Human Services | National Institutes of Health (NIH)/ GR - CA181486/U.S. Department of Defense (United States Department of Defense)/ GR - RP220391/Cancer Prevention and Research Institute of Texas (Cancer Prevention Research Institute of Texas)/ GR - RR160078/Cancer Prevention and Research Institute of Texas (Cancer Prevention Research Institute of Texas)/ GR - RP210028/Cancer Prevention and Research Institute of Texas (Cancer Prevention Research Institute of Texas)/ GR - Postdoctoral fellowship/American Cancer Society (American Cancer Society, Inc.)/ GR - 22 2186/Cancerfonden (Swedish Cancer Society)/ GR - 2013.0181/Knut och Alice Wallenbergs Stiftelse (Knut and Alice Wallenberg Foundation)/ GR - 231263/Radiumhemmets Forskningsfonder (Cancer Research Foundations of Radiumhemmet)/ GR - TFF-242122/Terry Fox Foundation/ GR - PJT-183843/Gouvernement du Canada | Canadian Institutes of Health Research (Instituts de Recherche en Sante du Canada)/ GR - PJT-451236/Gouvernement du Canada | Canadian Institutes of Health Research (Instituts de Recherche en Sante du Canada)/ GR - Regulation of mRNA Translation and Metabolism/Canada Research Chairs (Chaires de recherche du Canada)/ PT - Journal Article DEP - 20261029 PL - England TA - Nat Cell Biol JT - Nature cell biology JID - 100890575 SB - IM COIS- Competing interests: O.G. is a co-scientific founder and stockholder of EpiCypher, Inc. and K36 Therapeutics, Inc. P.K.M. is a consultant and stockholder of Ikena Oncology, Inc. and Alternative bio, Inc. O.G. is a board member of EpiCypher, Inc. S.C.B. holds equity interest in Lumidyne Technologies. The remaining authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:25 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/01/28 00:00 [received] PHST- 2026/09/08 00:00 [accepted] PHST- 2026/10/30 00:25 [entrez] AID - 10.1038/s41556-026-02085-6 [pii] AID - 10.1038/s41556-026-02085-6 [doi] PST - aheadofprint SO - Nat Cell Biol. 2026 Oct 29. doi: 10.1038/s41556-026-02085-6. PMID- 42957782 OWN - NLM STAT- Publisher LR - 20261030 IS - 1432-5195 (Electronic) IS - 0341-2695 (Linking) DP - 2026 Oct 30 TI - Musculoskeletal oncology practice in Latin America: Workforce characteristics, resource availability, and territorial disparities. LID - 10.1007/s00264-026-07064-y [doi] AB - PURPOSE: Musculoskeletal oncology requires highly specialized, multidisciplinary care. Although the subspecialty has evolved worldwide, information on workforce characteristics, resource availability, and practice patterns across Latin America remains limited. METHODS: A cross-sectional survey using convenience sampling was conducted among orthopaedic surgeons involved in musculoskeletal oncology throughout Latin America. An adapted version of a previously published questionnaire was distributed through the Latin American Society of Musculoskeletal Tumors (SLATME) and professional networks. The survey explored training background, clinical practice characteristics, perceived support from selected specialties and specialized resources, perceived professional challenges, and treatment preferences. We performed descriptive analyses and comparisons by practice-location population size (< 1 million versus >/= 1 million inhabitants). RESULTS: A total of 147 surgeons from 17 countries participated. Most respondents reported formal orthopaedic oncology training (76%) and academic orteaching involvement (93%). Respondents practicing in locations with >/= one million inhabitants were significantly more likely to report treating >/= 30 cases annually of soft tissue sarcoma (47% vs. 14%, p < 0.001), metastatic bone disease (73% vs. 40%, p < 0.001), and primary bone sarcoma (29% vs. 9%, p = 0.013). Access to molecular genetic testing, bone banks and allografts, and tumour reconstruction prostheses was also significantly greater in larger population centers, whereas access to MRI, radiotherapy, and intensive care did not differ significantly between groups. CONCLUSION: Among survey respondents, musculoskeletal oncology practice in Latin America was characterized by high rates of specialized training and academic or teaching involvement. However, differences in the reported annual numbers of new malignant tumour cases treated and in access to advanced resources were observed according to practice-location population size. Because this study used a convenience sampling strategy, these findings reflect the respondents' practices but should not be generalized to the entire Latin American musculoskeletal oncology community. CI - (c) 2026. The Author(s) under exclusive licence to SICOT aisbl. FAU - Zamora, Tomas AU - Zamora T AUID- ORCID: 0000-0003-3791-0449 AD - Department of Orthopaedic Surgery, Pontificia Universidad Catolica de Chile, Santiago, Chile. tezamora@uc.cl. AD - Red de Salud UC-CHRISTUS, Santiago, Chile. tezamora@uc.cl. FAU - Vidal, Catalina AU - Vidal C AUID- ORCID: 0000-0002-4700-7360 AD - Department of Orthopaedic Surgery, Pontificia Universidad Catolica de Chile, Santiago, Chile. FAU - Suarez, Hernan AU - Suarez H AD - Department of Orthopaedic Surgery, Pontificia Universidad Catolica de Chile, Santiago, Chile. FAU - Botello, Eduardo AU - Botello E AD - Department of Orthopaedic Surgery, Pontificia Universidad Catolica de Chile, Santiago, Chile. AD - Red de Salud UC-CHRISTUS, Santiago, Chile. FAU - Galli Serra, Marcos AU - Galli Serra M AD - Facultad de Ciencias Biomedicas, Universidad Austral., Hospital Universitario Austral, Pilar, Argentina. FAU - Casales, Nicolas AU - Casales N AD - Instituto Nacional de Ortopedia y Traumatologia, Montevideo, Uruguay. FAU - Zeballos, Joaquin AU - Zeballos J AD - Centro Bolivariano de Tumores Oseos, Cochabamba, Bolivia. FAU - Zumarraga, Juan Pablo AU - Zumarraga JP AD - Hospital Metropolitano, Quito, Ecuador. FAU - Cuervo, Carlos AU - Cuervo C AD - Hospital Zambrano Hellion, Monterrey, Mexico. FAU - Linares, Francisco AU - Linares F AD - Pontificia Universidad Javeriana, Bogota, Colombia. LA - eng PT - Journal Article DEP - 20261030 PL - Germany TA - Int Orthop JT - International orthopaedics JID - 7705431 SB - IM OTO - NOTNLM OT - Bone Neoplasms OT - Health resources OT - Latin America OT - Sarcoma OT - Soft tissue neoplasms COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:25 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/06/08 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/30 00:25 [entrez] AID - 10.1007/s00264-026-07064-y [pii] AID - 10.1007/s00264-026-07064-y [doi] PST - aheadofprint SO - Int Orthop. 2026 Oct 30. doi: 10.1007/s00264-026-07064-y. PMID- 42957781 OWN - NLM STAT- Publisher LR - 20261030 IS - 1432-5195 (Electronic) IS - 0341-2695 (Linking) DP - 2026 Oct 29 TI - Clinical decision-making for decompression-level selection in multilevel degenerative lumbar spinal stenosis: a retrospective cohort study. LID - 10.1007/s00264-026-07065-x [doi] AB - PURPOSE: To characterise changes in decompression-level selection during sequential preoperative assessment in patients with multilevel degenerative lumbar spinal stenosis (DLSS) and to describe postoperative outcomes and subsequent events involving initially untreated candidate levels. METHODS: This retrospective single-centre cohort included 168 patients with two to four MRI-defined candidate levels who underwent endoscopic decompression. Planned decompression levels were reconstructed before walking load testing (WLT), after WLT, after selective nerve root block (SNRB) when performed, and at the final preoperative assessment. Postoperative clinical outcomes and subsequent events involving initially untreated candidate levels were recorded during 24 months of follow-up. RESULTS: Planned level sets changed after WLT in 72/168 patients (42.9%), and final plans differed from initial imaging-informed plans in 87/168 patients (51.8%). Among 55 patients undergoing SNRB, post-WLT plans were modified in 22 (40.0%). All final planned levels were decompressed. At 24 months, 143 patients (85.1%) completed follow-up, with a model-estimated 38.44-point improvement in the Oswestry Disability Index (ODI); 123/143 (86.0%) achieved the ODI threshold for patient acceptable symptom state (PASS). Among 231 initially untreated candidate levels, five underwent delayed decompression, with a 24-month Kaplan-Meier estimate of 2.6% (95% CI 1.1-6.0). CONCLUSION: Sequential incorporation of imaging, functional assessment, and selective diagnostic procedures was associated with changes in planned decompression levels in patients with multilevel DLSS. This study describes surgical decision-making and the subsequent clinical course of initially untreated candidate levels after selective decompression planning. CI - (c) 2026. The Author(s) under exclusive licence to SICOT aisbl. FAU - Long, Yan AU - Long Y AUID- ORCID: 0009-0006-6202-5109 AD - The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Nanning, China. longy2510@gmail.com. FAU - Li, Lin AU - Li L AUID- ORCID: 0009-0005-7879-0967 AD - The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Nanning, China. FAU - Zhong, Yuanming AU - Zhong Y AD - The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Nanning, China. zym196395@sina.com. FAU - Li, Chengwei AU - Li C AD - Beiliu City Traditional Chinese Medicine Hospital, Beiliu, Guangxi, China. AD - The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Nanning, China. FAU - Yang, Shichang AU - Yang S AD - The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Nanning, China. FAU - Wang, Guancheng AU - Wang G AD - The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Nanning, China. FAU - Sun, Haoran AU - Sun H AD - The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Nanning, China. FAU - Si, Jie AU - Si J AD - The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Nanning, China. FAU - Li, Jinyu AU - Li J AD - The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Nanning, China. FAU - Zhang, Yisheng AU - Zhang Y AD - The First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine, Nanning, China. 996913937@qq.com. LA - eng GR - No. Guike AB20159018/Guangxi Key Research and Development Program/ PT - Journal Article DEP - 20261029 PL - Germany TA - Int Orthop JT - International orthopaedics JID - 7705431 SB - IM OTO - NOTNLM OT - Degenerative lumbar spinal stenosis OT - Endoscopic decompression OT - Patient acceptable symptom state OT - Selective nerve root block OT - Surgical decision-making OT - Walking load test COIS- Declarations. Ethics approval and consent to participate: Ethics approval and consent to participate The study was approved by the Institutional Ethics Committee of the First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine (approval No. 2022-084-02). The study was conducted in accordance with the approved protocol and the principles of the Declaration of Helsinki. Owing to the retrospective design of the study and the use of anonymised data, the requirement for informed consent was waived by the Ethics Committee. Clinical trial number: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:25 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/12 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/30 00:25 [entrez] AID - 10.1007/s00264-026-07065-x [pii] AID - 10.1007/s00264-026-07065-x [doi] PST - aheadofprint SO - Int Orthop. 2026 Oct 29. doi: 10.1007/s00264-026-07065-x. PMID- 42957780 OWN - NLM STAT- Publisher LR - 20261030 IS - 1432-2161 (Electronic) IS - 0364-2348 (Linking) DP - 2026 Oct 29 TI - Ghost cell odontogenic carcinoma: a case report and literature review. LID - 10.1007/s00256-026-05416-8 [doi] AB - Ghost cell odontogenic carcinoma (GCOC) is an exceedingly rare malignant odontogenic epithelial tumor, distinguished by the presence of ghost cells. GCOC exhibits nonspecific clinical and radiologic features, such as variable pain, swelling, and osteolytic destruction without distinct periosteal reaction, and can be locally destructive and invasive, with the potential for distant metastases. Currently, no effective systemic therapy is recommended for patients. We present a case of a 56-year-old male patient diagnosed with GCOC involving the maxilla, along with a review of the previous literature. The patient underwent en bloc resection of the tumor. However, the tumor recurred 3 months later, and the patient opted for conservative management. Unfortunately, the patient passed away 2 weeks after the recurrence. Based on the case presentation and literature review, we conclude that GCOC represents an aggressive malignancy with a high potential for local recurrence and metastatic disease. This necessitates preoperative accurate imaging diagnosis to guide radical resection, and highlights the need for effective adjuvant therapies, which are currently unavailable, to prevent rapid disease progression and mortality. CI - (c) 2026. The Author(s), under exclusive licence to International Skeletal Society (ISS). FAU - He, Yao AU - He Y AD - Department of Radiology, The 940 Hospital of Joint Logistic Support Force of Chinese People's Liberation Army, Lanzhou, 730000, China. FAU - Xue, Wei AU - Xue W AD - Department of Radiology, The 940 Hospital of Joint Logistic Support Force of Chinese People's Liberation Army, Lanzhou, 730000, China. FAU - Feng, Jun AU - Feng J AD - Northwest Minzu University, Lanzhou, 730000, China. FAU - Chen, Gang AU - Chen G AD - Department of Radiology, The 940 Hospital of Joint Logistic Support Force of Chinese People's Liberation Army, Lanzhou, 730000, China. FAU - Ma, Dongyang AU - Ma D AD - Department of Oral and Maxillofacial Surgery, The 940 Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Lanzhou, 730000, China. Doctormdy@hotmail.com. FAU - Niu, Juanqin AU - Niu J AD - Department of Radiology, The 940 Hospital of Joint Logistic Support Force of Chinese People's Liberation Army, Lanzhou, 730000, China. 461654179@qq.com. AD - Northwest Minzu University, Lanzhou, 730000, China. 461654179@qq.com. LA - eng GR - 2023HQZZ/the Technological Innovation Plan and Innovation Team Project of PLA/ GR - 2023YXKY005/the Technological Innovation Plan and Innovation Team Project of PLA/ PT - Journal Article DEP - 20261029 PL - Germany TA - Skeletal Radiol JT - Skeletal radiology JID - 7701953 SB - IM OTO - NOTNLM OT - Computed tomography OT - Ghost cell OT - Ghost cell odontogenic carcinoma OT - Magnetic resonance imaging COIS- Declarations. Ethics approval and consent to participate: This study was approved by the Ethics Committee of The 940th Hospital of Joint Logistic Support Force of Chinese People's Liberation Army (Lanzhou, China) and was conducted in accordance with the principles of the Helsinki Declaration. Consent for publication: Written informed consent from study participant for publication of their cases and accompanying images. Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:25 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/18 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/30 00:25 [entrez] AID - 10.1007/s00256-026-05416-8 [pii] AID - 10.1007/s00256-026-05416-8 [doi] PST - aheadofprint SO - Skeletal Radiol. 2026 Oct 29. doi: 10.1007/s00256-026-05416-8. PMID- 42957778 OWN - NLM STAT- Publisher LR - 20261030 IS - 1546-170X (Electronic) IS - 1078-8956 (Linking) DP - 2026 Oct 29 TI - 5-year outcomes of chemotherapy de-escalation in HER2-positive early breast cancer and exploratory circulating tumor DNA analyses from the randomized phase 2 PHERGain trial. LID - 10.1038/s41591-026-04723-w [doi] AB - PHERGain was a randomized, non-comparative phase 2 trial evaluating a positron (18)F-fluorodeoxyglucose-positron emission tomography ((18)F-FDG-PET)-guided, pathological complete response (pCR)-adapted strategy to omit chemotherapy in HER2-positive early breast cancer. Female patients with HER2-positive stages I-IIIA operable breast cancer (tumor size >/= 1.5 cm) were randomized to receive chemotherapy plus trastuzumab and pertuzumab (HP; group A) or HP +/- endocrine therapy (group B). In group B, treatment was adapted according to (18)F-FDG-PET response after two cycles and pCR, with chemotherapy administered to nonresponders or patients without pCR. We previously reported that this de-escalation approach revealed a 3-year invasive disease-free survival (iDFS) of 94.3% (95% confidence interval (CI): 90.9 to 96.8) in group B. Here we report the prespecified secondary endpoint, 5-year iDFS and exploratory circulating tumor DNA (ctDNA) findings. After a median follow-up of 5.6 years, the 5-year iDFS was 89.5% (95% CI: 85.7 to 93.4) in group B (n = 267) and 92.4% (95% CI: 86.7 to 98.5) among (18)F-FDG-PET responders achieving pCR without chemotherapy (n = 86), with one distant recurrence. The latter estimate describes a long-term outcome in a post-randomization subgroup defined by (18)F-FDG-PET response and pCR and should be interpreted as descriptive rather than as causal effects of omitting chemotherapy. Detectable ctDNA at baseline or during treatment was associated with inferior iDFS, whereas ctDNA clearance correlated with higher pCR rates and improved long-term outcomes. These findings continue to support the clinical validity of an (18)F-FDG-PET-guided, pCR-adapted approach and suggest a potential role for ctDNA in refining treatment individualization. ClinicalTrials.gov identifier: NCT03161353 . CI - (c) 2026. The Author(s). FAU - Perez-Garcia, Jose Manuel AU - Perez-Garcia JM AD - Medica Scientia Innovation Research (MedSIR), Barcelona, Spain. AD - International Breast Cancer Center (IBCC), Pangaea Oncology, Quiron Group, Barcelona, Spain. FAU - Cortes, Javier AU - Cortes J AUID- ORCID: 0000-0001-7623-1583 AD - Medica Scientia Innovation Research (MedSIR), Barcelona, Spain. AD - International Breast Cancer Center (IBCC), Pangaea Oncology, Quiron Group, Barcelona, Spain. AD - Department of Medicine, Universidad Europea de Madrid, Faculty of Biomedical and Health Sciences, Madrid, Spain. AD - IOB Madrid, Hospital Beata Maria Ana, Madrid, Spain. FAU - Ruiz-Borrego, Manuel AU - Ruiz-Borrego M AUID- ORCID: 0000-0002-1181-5622 AD - Medical Oncology Department, Virgen del Rocio University Hospital, Sevilla, Spain. FAU - Stradella, Agostina AU - Stradella A AD - Medical Oncology Department, Institut Catala D'Oncologia-IDIBELL, L'Hospitalet de Llobregat, Barcelona, Spain. FAU - Bermejo, Begona AU - Bermejo B AD - Medical Oncology, Hospital Clinico Universitario de Valencia, Biomedical Research Institute INCLIVA, Valencia, Spain. AD - Oncology Biomedical Research National Network (CIBERONC-ISCIII), Madrid, Spain. AD - Medicine Department, Universidad de Valencia, Valencia, Spain. FAU - Escriva-de-Romani, Santiago AU - Escriva-de-Romani S AUID- ORCID: 0000-0001-7816-7589 AD - Medical Oncology Department, Breast Cancer Group, Vall d'Hebron University Hospital, Vall d'Hebron Institute of Oncology (VHIO), Barcelona, Spain. FAU - Reboredo, Cristina AU - Reboredo C AD - Hospital Universitario A Coruna, Breast Cancer Unit, A Coruna, Spain. FAU - Albacar, Cinta AU - Albacar C AUID- ORCID: 0000-0002-4144-3348 AD - Medical Oncology Unit, Institut d'Oncologia de la Catalunya Sud (IOCS), Hospital Universitari Sant Joan de Reus, Institut Investigacio Sanitaria Pere Virgili (IISPV), Reus, Spain. FAU - Martinez-Janez, Noelia AU - Martinez-Janez N AD - Medical Oncology Department, Hospital Universitario Ramon y Cajal (IRYCIS), Madrid, Spain. FAU - Iranzo, Vega AU - Iranzo V AUID- ORCID: 0000-0001-6183-5173 AD - Consorci Hospital General Universitari de Valencia, Valencia, Spain. FAU - Ribelles, Nuria AU - Ribelles N AD - Servicio de Oncologia Medica, Hospital Universitario Virgen de la Victoria, Instituto de Investigaciones Biomedicas de Malaga (IBIMA), Malaga, Spain. FAU - Dalenc, Florence AU - Dalenc F AD - Department of Medical Oncology, Oncopole Claudius Regaud, IUCT, Toulouse, France. FAU - Prat, Aleix AU - Prat A AUID- ORCID: 0000-0003-2377-540X AD - Department of Medical Oncology, Hospital Clinic of Barcelona, Barcelona, Spain. AD - Translational Genomics and Targeted Therapies Group, IDIBAPS, Barcelona, Spain. AD - Department of Medicine, University of Barcelona, Barcelona, Spain. FAU - Schmid, Peter AU - Schmid P AUID- ORCID: 0000-0001-9817-5228 AD - Barts Experimental Cancer Medicine Centre, Barts Cancer Institute, Queen Mary University of London, London, UK. AD - Barts Hospital NHS Trust, London, UK. FAU - Gebhart, Geraldine AU - Gebhart G AD - Institut Jules Bordet-Universite Libre de Bruxelles, Brussels, Belgium. FAU - Marme, Frederik AU - Marme F AUID- ORCID: 0000-0002-6591-3367 AD - Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany. FAU - Di Cosimo, Serena AU - Di Cosimo S AD - Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy. FAU - Braga, Sofia AU - Braga S AD - Hospital Professor Doutor Fernando Fonseca EPE, Lisbon, Portugal. FAU - Colleoni, Marco AU - Colleoni M AD - Instituto Europeo di Oncologia (IEO), IRCCS, Milan, Italy. FAU - Antonarelli, Gabriele AU - Antonarelli G AD - Department of Oncology and Hemato-Oncology (DIPO), University of Milan, Milan, Italy. AD - Division of Early Drug Development for Innovative Therapies, European Institute of Oncology, IRCCS, Milan, Italy. FAU - Notini, Giulia AU - Notini G AD - Medica Scientia Innovation Research (MedSIR), Barcelona, Spain. AD - International Breast Cancer Center (IBCC), Pangaea Oncology, Quiron Group, Barcelona, Spain. AD - Department of Medical Oncology, IRCCS San Raffaele Hospital, Milan, Italy. AD - School of Medicine and Surgery, Vita-Salute San Raffaele University, Milan, Italy. FAU - Gion, Maria AU - Gion M AD - IOB Madrid, Hospital Beata Maria Ana, Madrid, Spain. AD - University Hospital Ramon y Cajal, Madrid, Spain. FAU - Garcia-Mosquera, Juan Jose AU - Garcia-Mosquera JJ AUID- ORCID: 0000-0001-5165-4168 AD - Medica Scientia Innovation Research (MedSIR), Barcelona, Spain. AD - Dr. Rosell Oncology Institute (IOR), Dexeus University Hospital, Pangaea Oncology, Quironsalud Group, Barcelona, Spain. FAU - Sanz, Lucia AU - Sanz L AD - IOB Madrid, Hospital Beata Maria Ana, Madrid, Spain. FAU - Martinez-Garcia, Elena AU - Martinez-Garcia E AD - Medica Scientia Innovation Research (MedSIR), Barcelona, Spain. FAU - Meya, Alba AU - Meya A AD - Medica Scientia Innovation Research (MedSIR), Barcelona, Spain. FAU - Dustin, Derek AU - Dustin D AD - Guardant Health, Palo Alto, CA, USA. FAU - Amaya-Garrido, Ana AU - Amaya-Garrido A AD - Medica Scientia Innovation Research (MedSIR), Barcelona, Spain. FAU - Guerrero-Martinez, Jose Antonio AU - Guerrero-Martinez JA AD - Medica Scientia Innovation Research (MedSIR), Barcelona, Spain. FAU - Alcala-Lopez, Daniel AU - Alcala-Lopez D AD - Medica Scientia Innovation Research (MedSIR), Barcelona, Spain. FAU - Rodriguez-Morato, Jose AU - Rodriguez-Morato J AUID- ORCID: 0000-0002-8133-9983 AD - Medica Scientia Innovation Research (MedSIR), Barcelona, Spain. FAU - Mina, Leonardo AU - Mina L AD - Medica Scientia Innovation Research (MedSIR), Barcelona, Spain. FAU - Mancino, Mario AU - Mancino M AD - Medica Scientia Innovation Research (MedSIR), Barcelona, Spain. FAU - Llombart-Cussac, Antonio AU - Llombart-Cussac A AUID- ORCID: 0000-0003-4515-8293 AD - Medica Scientia Innovation Research (MedSIR), Barcelona, Spain. antonio.llombart@maj3.health. AD - Hospital Arnau de Vilanova, FISABIO, Valencia, Spain. antonio.llombart@maj3.health. AD - Translational Oncology Group, Department of Medicine, Facultad de Ciencias de la Salud, Universidad Cardenal Herrera-CEU, Alfara del Patriarca, Spain. antonio.llombart@maj3.health. LA - eng SI - ClinicalTrials.gov/NCT03161353 PT - Journal Article DEP - 20261029 PL - United States TA - Nat Med JT - Nature medicine JID - 9502015 SB - IM COIS- Competing interests: J.M.P.-G. declares honoraria for advisory board participation from Eli Lilly and Company, Roche, Eisai, Daiichi Sankyo, AstraZeneca, Seattle Genetics, Merck Sharp & Dohme and Gilead; personal support for attending meetings or travel, accommodation and expenses from Roche; and is an employee at MEDSIR. J.C. declares honoraria from Roche, Novartis, Eisai, Pfizer, Eli Lilly and Company, Merck Sharp & Dohme, Daiichi Sankyo, AstraZeneca, Gilead, Steamline Therapeutics and Zuellig Pharma; consulting/advisory work for Roche, AstraZeneca, Seattle Genetics, Daiichi Sankyo, Eli Lilly and Company, Merck Sharp & Dohme, Leuko, Bioasis, Clovis Oncology, Boehringer Ingelheim, Ellipses, Hibercell, BioInvent, Gemoab, Gilead, Menarini, Zymeworks, Reveal Genomics, Scorpion Therapeutics, Expres2ion Biotechnologies, Jazz Pharmaceuticals, Abbvie, BridgeBio, BioNTech, Biocon, Circle Pharma, Delcath Systems, Hexagon Bio, Bliss Biopharmaceutical and Relay Therapeutics; research funding to their institution from Roche, ARIAD Pharmaceuticals, AstraZeneca, Baxalta/Servier Affaires, Bayer Healthcare, Eisai, F. Hoffman-La Roche, Guardant Health, Merck Sharp & Dohme, Pfizer, Piqur Therapeutics, IQVIA and Queen Mary University of London; stock from MAJ3 Capital, Leuko (relative); and travel, accommodation and expenses from Roche, Novartis, Eisai, Pfizer, Daiichi Sankyo, AstraZeneca, Gilead, Merck Sharp & Dhome and Steamline Therapeutics. Patents: 'Pharmaceutical combinations of a Pi3k inhibitor and a microtubule destabilizing agent'. Javier Cortes Castan, Alejandro Piris Gimenez, Violeta Serra Elizalde. WO 2014/199294 A. ISSUED; 'Her2 as a predictor of response to dual HER2 blockade in the absence of cytotoxic therapy'. Aleix Prat, Antonio Llombart, Javier Cortes. US 2019/ 0338368 A1. LICENSED. M.R.-B. declares having received speaker grants from Daiichi Sankyo, AstraZeneca, Novartis, Eli Lilly and Company, Pfizer, Gilead and Roche; advisory board fees from Daiichi Sankyo, AstraZeneca, Pfizer, Gilead and MSD; and travel fund from Pfizer, Novartis, Roche, Daiichi Sankyo and AstraZeneca. A.S. declares having received honoraria for speaker bureau from Daiichi Sankyo, Novartis, AstraZeneca and Gilead; travel, accommodation and expenses from Eisai, Novartis, Pfizer, Gilead and Menarini; an advisory role for AstraZeneca, Novartis, MSD and Gilead; and being a member of GEICAM's Steering Committee. B.B. declares having received honoraria for advisory board participation from Novartis, Eli Lilly and Company, Menarini and AstraZeneca; speaker's bureau from Genetec, Pfizer, Merck Sharp & Dohme and Daiichi Sankyo; and personal support for travel, accommodation and expenses from Gilead Sciences and Pfizer. S.E.-d.-R. declares having received honoraria from COR2ED, Daiichi Sankyo, AstraZeneca, Jazz Pharmaceuticals, Novartis, Pfizer, Roche and Seagen; consulting/advisory roles for Daiichi Sankyo, AstraZeneca, Jazz Pharmaceuticals, Pfizer, Pierre-Fabre, Reveal Genomics and Roche; travel, accommodation and expenses from Daiichi Sankyo, Pfizer and Seagen; and research funding to their institution as local principal investigator for studies sponsored by Byondis, Daiichi Sankyo, AstraZeneca, Jazz Pharmaceuticals, Eli Lilly and Company, MedSIR, Roche, SOLTI, Synthon and Zymeworks. N.M.-J., E.M.-G., A.M., A.A.-G., J.A.G.-M., D.A.-L., J.R.-M., L.M. and M.M. declare being employees of MEDSIR. V.I. declares having received honoraria for consulting or advisory board participation from AstraZeneca and MSD Oncology, and travel, accommodation and expenses form Pfizer, AstraZeneca, Novartis, Roche and Gilead Sciences. N.R. declares having received honoraria for speakers' bureau from Novartis, Pfizer, Daiichi Sankyo, AstraZeneca and Gilead, and research funding from Pfizer. A.P. reports advisory and consulting fees from AstraZeneca, Roche, Pfizer, Novartis, Daiichi Sankyo, Ona Therapeutics and Peptomyc; lecture fees from AstraZeneca, Roche, Novartis and Daiichi Sankyo; institutional financial interests from AstraZeneca, Novartis, Roche and Daiichi Sankyo; being a stockholder and employee of Reveal Genomics; and patents filed (PCT/EP2016/080056, PCT/EP2022/086493, PCT/EP2023/060810, PCT/EP2024/068197 and EP23383369). P.S. declares having received personal honoraria for advisory board participation from AstraZeneca, Bicycle Therapeutics, Daiichi Sankyo, Gilead, Eli Lilly and Company, MSD, Novartis, Olema Oncology, Pfizer, Roche and Summit Therapeutics; and personal honoraria as an invited speaker from AstraZeneca, Daiichi Sankyo, Gilead, Menarini Stemline, MSD and Roche; and institutional research grants (financial interests) from AstraZeneca, Genentech and Roche. F.M. declares having received personal honoraria for advisory board participation from AstraZeneca, BioNTech, Daiichi Sankyo, Eisai, Genomic Health, Gilead Sciences, Immunicom, Incyte, MSD, Myriad, Nerviano, Novartis, Novocure, Roche and Seagen; and personal honoraria as an invited speaker from AstraZeneca, Daiichi Sankyo, GSK, Eli Lilly and Company and Pfizer; and institutional honoraria as an invited speaker from AstraZeneca, Daiichi Sankyo, GSK, Seagen and Stemline Menarini. In addition, he has served as coordinating or local principal investigator for clinical studies sponsored by multiple organizations, including AGO Research, AstraZeneca, Boehringer Ingelheim, Daiichi Sankyo, Eisai, German Breast Group, Gilead Sciences, GSK, Eli Lilly and Company, Menarini Stemline, MSD, Novartis, Roche, Seagen and Vaccibody; and institutional funding with financial interest from Boehringer Ingelheim, Daiichi Sankyo and Menarini Stemline. S.D.C. declares having received an institutional grant from the Ministry of Health for project PNRR-POC-2023-12378113; serving as an 'ad hoc' medical monitor for MEDSIR; support for attending meetings or travel from Daiichi Sankyo; and participation on an advisory board for Pfizer. S.B. declares having received honoraria for consulting or advisory board participation from Daiichi Sankyo, AstraZeneca, Novartis and Roche; speaker/conference fees from Daiichi Sankyo, AstraZeneca, Novartis and Roche; and travel fees from Daiichi Sankyo, AstraZeneca, Novartis and Roche. G.A. declares having received honoraria from MEDSIR. G.N. declares having received honoraria for travel, accommodation and expenses from Eli Lilly and Company, MSD and Novartis. M.G. declares having received honoraria from Novartis, Gilead, AstraZeneca and Pfizer; personal support for attending meetings or travel from Roche, Pfizer, AstraZeneca and Gilead; and honoraria for advisory board participation from Gilead, Novartis, AstraZeneca and Pfizer. J.J.G.-M. declares receiving honoraria for consulting or advisory role from MEDSIR; travel, accommodation and expenses from Novartis, Eli Lilly and Company, Roche and Daiichi Sankyo Europe; and being an employee at Trialing Health S.L. A.L.C. declares having received honoraria for research support from Roche, Agendia, Eli Lilly and Company, Pfizer, Novartis, Merck Sharp & Dhome, Gilead Sciences and Daiichi Sankyo; consulting/advisory work for Eli Lilly and Company, Roche, Pfizer, and Novartis; speaker's bureau fees from Eli Lilly and Company, AstraZeneca, Merck Sharp & Dhome, Pfizer and Novartis; travel support from Roche, Pfizer, AstraZeneca, Steamline Therapeutics and Merck Sharp & Dhome. Patents: 'Her2 as a predictor of response to dual HER2 blockade in the absence of cytotoxic therapy'. Aleix Prat, Antonio Llombart, Javier Cortes. US 2019/0338368 A1. LICENSED; and Stock or other ownership from MAJ3 CAPITAL & Initia. The other authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:25 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/17 00:00 [received] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/30 00:25 [entrez] AID - 10.1038/s41591-026-04723-w [pii] AID - 10.1038/s41591-026-04723-w [doi] PST - aheadofprint SO - Nat Med. 2026 Oct 29. doi: 10.1038/s41591-026-04723-w. PMID- 42957771 OWN - NLM STAT- Publisher LR - 20261030 IS - 1474-1741 (Electronic) IS - 1474-1733 (Linking) DP - 2026 Oct 28 TI - Granzyme K-expressing CD8(+) T cells, complement activation and histotoxic immunity. LID - 10.1038/s41577-026-01364-5 [doi] AB - Cytotoxic CD8(+) T cells induce target-cell death by producing effector proteases called granzymes. Most granzymes, as exemplified by granzyme B, induce apoptosis by cleaving intracellular substrates after delivery to target cells via the immunological synapse. By contrast, it was recently shown that granzyme K (GZMK), which is selectively expressed by a unique population of CD8(+) T cells, activates the complement cascade instead of executing direct cytotoxicity. In this Perspective, we summarize recent data on complement activation by CD8(+) T cell-derived GZMK that points to a novel lymphocyte-mediated pathway of complement activation. We present evidence that GZMK-expressing CD8(+) T cells have distinct pro-inflammatory effects in tissues and discuss their involvement in an array of chronic and inflammatory diseases. Furthermore, we propose the concept of 'tissue histotoxicity' as a core feature of GZMK-expressing CD8(+) T cells. We suggest this as a defence strategy that enables the immune system to act on a tissue neighbourhood instead of targeting one cell at a time. Finally, we discuss the possibility of GZMK expression being a heritable trait in subsets of memory T cells. CI - (c) 2026. Springer Nature Limited. FAU - Liu, Xin AU - Liu X AUID- ORCID: 0000-0002-6634-4012 AD - School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China. xil2020@tsinghua.edu.cn. AD - Laboratory of Dynamic Immunobiology, Institute for Immunology, Tsinghua University, Beijing, China. xil2020@tsinghua.edu.cn. FAU - Qi, Hai AU - Qi H AUID- ORCID: 0000-0001-5475-3989 AD - School of Basic Medical Sciences, Tsinghua Medicine, Tsinghua University, Beijing, China. qihai@tsinghua.edu.cn. AD - Laboratory of Dynamic Immunobiology, Institute for Immunology, Tsinghua University, Beijing, China. qihai@tsinghua.edu.cn. AD - Tsinghua-Peking Center for Life Sciences, Beijing, China. qihai@tsinghua.edu.cn. AD - Changping Laboratory, Yard 28, Science Park Rd., Changping District, Beijing, China. qihai@tsinghua.edu.cn. AD - New Cornerstone Science Laboratory, Tsinghua Medicine, Tsinghua University, Beijing, China. qihai@tsinghua.edu.cn. AD - Beijing Frontier Research Center for Biological Structure, Tsinghua University, Beijing, China. qihai@tsinghua.edu.cn. AD - Beijing Key Laboratory of Immunotherapy for Allergic Diseases, Tsinghua University, Beijing, China. qihai@tsinghua.edu.cn. AD - SXMU-Tsinghua Collaborative Innovation Center for Frontier Medicine, School of Medicine, Tsinghua University, Beijing, China. qihai@tsinghua.edu.cn. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - Nat Rev Immunol JT - Nature reviews. Immunology JID - 101124169 SB - IM COIS- Competing interests: H.Q. is a cofounder of Emergent Biomed Solutions, Ltd. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:24 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/30 00:24 [entrez] AID - 10.1038/s41577-026-01364-5 [pii] AID - 10.1038/s41577-026-01364-5 [doi] PST - aheadofprint SO - Nat Rev Immunol. 2026 Oct 28. doi: 10.1038/s41577-026-01364-5. PMID- 42957754 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261030 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 29 TI - Domino effect enables dynamic electronic structure evolution for stable and efficient water purification. LID - 11214 [pii] LID - 10.1038/s41467-026-78263-9 [doi] AB - The purification of water contaminated by persistent organic pollutants remains a critical global challenge, largely due to the intrinsic trade-off between high catalytic activity and long-term stability in advanced oxidation processes. Despite the tremendous potential of single-atom catalysis, its traditional static design strategies cannot adapt to dynamic and multi-step reactions. Here, we break the limitation of this static design by reporting a domino catalysis within a heteronuclear MgCo dual-single-atom catalyst. In this system, the Mg site selectively photoreduces O(2) to H(2)O(2) under light irradiation. This in-situ generated H(2)O(2) and its derived surface hydroxyl groups act as dynamic modulators, progressively tailoring the electronic structure of the adjacent Co site. This on-demand electronic refinement optimally lowers the energy barriers for subsequent peroxymonosulfate activation and radical desorption, thereby driving efficient pollutant degradation while intrinsically stabilizing the Co center against leaching. Experiments and theoretical calculations elucidate that this seamless and spatially ordered cascade avoids competitive adsorption and maximizes efficiency. The system exhibits the highest normalized rate constant of 595.3 min(-1) M(-1) among various refractory contaminants over thirty regeneration cycles, outperforming the most state-of-the-art catalytic systems. This work introduces a dynamic and self-evolving catalytic approach that breaks the traditional activity-stability trade-off, suggesting a potential pathway toward effective and sustainable water purification technologies. CI - (c) 2026. The Author(s). FAU - Jiang, Jingjing AU - Jiang J AD - Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin University, Changchun, China. AD - Jilin Provincial Key Laboratory of Water Resources and Environment, Jilin University, Changchun, China. FAU - He, Chang AU - He C AD - Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin University, Changchun, China. AD - Jilin Provincial Key Laboratory of Water Resources and Environment, Jilin University, Changchun, China. FAU - Liu, Shengda AU - Liu S AD - School of Chemical and Environmental Engineering, Changchun University of Science and Technology, Changchun, China. FAU - Sun, Tongze AU - Sun T AD - Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin University, Changchun, China. AD - Jilin Provincial Key Laboratory of Water Resources and Environment, Jilin University, Changchun, China. FAU - Zhao, Bowen AU - Zhao B AD - Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin University, Changchun, China. AD - Jilin Provincial Key Laboratory of Water Resources and Environment, Jilin University, Changchun, China. FAU - Wang, Ruixin AU - Wang R AD - Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin University, Changchun, China. AD - Jilin Provincial Key Laboratory of Water Resources and Environment, Jilin University, Changchun, China. FAU - Zhang, Yanan AU - Zhang Y AD - Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin University, Changchun, China. AD - Jilin Provincial Key Laboratory of Water Resources and Environment, Jilin University, Changchun, China. FAU - Zhao, Zhenhao AU - Zhao Z AD - Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin University, Changchun, China. AD - Jilin Provincial Key Laboratory of Water Resources and Environment, Jilin University, Changchun, China. FAU - Ye, Xuesong AU - Ye X AD - Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin University, Changchun, China. AD - Jilin Provincial Key Laboratory of Water Resources and Environment, Jilin University, Changchun, China. FAU - Yu, Hao AU - Yu H AD - Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin University, Changchun, China. AD - Jilin Provincial Key Laboratory of Water Resources and Environment, Jilin University, Changchun, China. FAU - Fu, Yifang AU - Fu Y AD - Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin University, Changchun, China. AD - Jilin Provincial Key Laboratory of Water Resources and Environment, Jilin University, Changchun, China. FAU - Liu, Yanan AU - Liu Y AD - Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin University, Changchun, China. AD - Jilin Provincial Key Laboratory of Water Resources and Environment, Jilin University, Changchun, China. FAU - Wu, Di AU - Wu D AD - Centre for Environment and Energy Research, Ghent University Global Campus, Incheon, South Korea. AD - Department of Green Chemistry and Technology, Ghent University, and Centre for Advanced Process Technology for Urban Resource Recovery, Ghent, Belgium. FAU - Huo, Mingxin AU - Huo M AD - Engineering Lab for Water Pollution Control and Resources Recovery of Jilin Province, School of Environment, Northeast Normal University, Changchun, China. FAU - Zhou, Dandan AU - Zhou D AD - Engineering Lab for Water Pollution Control and Resources Recovery of Jilin Province, School of Environment, Northeast Normal University, Changchun, China. FAU - Dong, Shuangshi AU - Dong S AUID- ORCID: 0000-0002-7103-3438 AD - Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin University, Changchun, China. sdong@jlu.edu.cn. AD - Jilin Provincial Key Laboratory of Water Resources and Environment, Jilin University, Changchun, China. sdong@jlu.edu.cn. LA - eng GR - 52570079/National Natural Science Foundation of China (National Science Foundation of China)/ GR - 52300088/National Natural Science Foundation of China (National Science Foundation of China)/ GR - W2521110/National Natural Science Foundation of China (National Science Foundation of China)/ GR - 52170079/National Natural Science Foundation of China (National Science Foundation of China)/ GR - 2023YFC3207704/National Natural Science Foundation of China (National Science Foundation of China)/ GR - 20260602011RC/Department of Science and Technology of Jilin Province (Jilin Province Science and Technology Department)/ PT - Journal Article DEP - 20260929 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 SB - IM COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:22 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/07 00:00 [received] PHST- 2026/09/14 00:00 [accepted] PHST- 2026/10/30 00:22 [entrez] AID - 10.1038/s41467-026-78263-9 [pii] AID - 10.1038/s41467-026-78263-9 [doi] PST - epublish SO - Nat Commun. 2026 Sep 29;17(1):11214. doi: 10.1038/s41467-026-78263-9. PMID- 42957752 OWN - NLM STAT- Publisher LR - 20261030 IS - 1476-5551 (Electronic) IS - 0887-6924 (Linking) DP - 2026 Oct 28 TI - Rapid proteasomal degradation of mutant feline McDonough sarcoma-like tyrosine kinase-3 overcomes inhibitor resistance mechanisms of acute myeloid leukemia cells. LID - 10.1038/s41375-026-03168-x [doi] AB - The hyperactive kinase FLT3-ITD causes acute myeloid leukemia (AML) and is a prognostic biomarker. We synthesized and characterized MA191 as a novel von Hippel-Lindau (VHL) E3 ubiquitin ligase-based proteolysis targeting chimera (PROTAC) for FLT3. MA191 induces apoptosis in cultured and primary AML patient cells harboring FLT3-ITD (IC(50)=9.6-11 nM; EC(50)=0.001-0.883 microM). Comparison with a VHL-binding-deficient stereoisomer of MA191 demonstrates that FLT3-ITD degradation is superior to kinase inhibition (p < 0.03). Rapid depletion of FLT3-ITD by MA191 (DC(50)=10 nM) requires VHL, neddylation, proteasome activity, and the pro-apoptotic BH3-only protein BIM. MA191 reduces FLT3-ITD prior to apoptosis, uncovering apoptosis-independent roles of BIM for protein stability. Higher expression levels of FLT3 in leukemia cells than in healthy cells (n = 3675/n = 1249) are associated with worse AML patient survival (p = 0.0099). MA191 does not harm healthy blood and bone marrow progenitor cells or myeloid cell differentiation. In Danio rerio larvae, MA191 halts AML cell proliferation without significant toxicity. Human stromal cell interactions and mutations in the tyrosine kinase domain of FLT3-ITD that confer resistance to FLT3 inhibitors do not abolish anti-leukemic effects of MA191. Accordingly, leukemia cells with acquired resistance to MA191 lack such mutations. We offer a framework for fast-acting next-generation FLT3 degraders that overcome resistance mechanisms to classical inhibitors. CI - (c) 2026. The Author(s). FAU - Halilovic, Melisa AU - Halilovic M AD - Institute of Toxicology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. FAU - Abdelsalam, Mohamed AU - Abdelsalam M AD - Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt. AD - Department of Medicinal Chemistry, Institute of Pharmacy, Martin-Luther-University of Halle-Wittenberg, Halle (Saale), Germany. FAU - Mieland, Andreas O AU - Mieland AO AD - Institute of Toxicology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. FAU - Neuroth, Sarah AU - Neuroth S AD - Institute of Toxicology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. FAU - Ashry, Ramy AU - Ashry R AD - Institute of Toxicology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. AD - Department of Oral Pathology, Faculty of Dentistry, Mansoura University, Mansoura, Egypt. FAU - Schwarting, Marie E S AU - Schwarting MES AD - Institute of Toxicology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. FAU - Zabkiewicz, Joanna AU - Zabkiewicz J AD - Department of Haematology, Cardiff Experimental Cancer Medicine Centre, University of Cardiff, Cardiff, UK. FAU - Lazenby, Michelle AU - Lazenby M AD - Department of Haematology, Cardiff Experimental Cancer Medicine Centre, University of Cardiff, Cardiff, UK. FAU - Alvares, Caroline AU - Alvares C AUID- ORCID: 0000-0003-4391-9802 AD - Department of Haematology, Cardiff Experimental Cancer Medicine Centre, University of Cardiff, Cardiff, UK. FAU - Schmidt, Matthias AU - Schmidt M AD - Department of Medicinal Chemistry, Institute of Pharmacy, Martin-Luther-University of Halle-Wittenberg, Halle (Saale), Germany. FAU - Marx, Christian AU - Marx C AUID- ORCID: 0000-0003-2240-8261 AD - Institute for Micro and Nanotechnologies (MacroNano(R)), Nano-Biosystem Technology, Ilmenau University of Technology, Ilmenau, Germany. FAU - Schober, Andreas AU - Schober A AD - Institute for Micro and Nanotechnologies (MacroNano(R)), Nano-Biosystem Technology, Ilmenau University of Technology, Ilmenau, Germany. FAU - Muller, Sylvia AU - Muller S AD - Institute of Immunology, Jena University Hospital, Friedrich-Schiller-University, Jena, Germany. FAU - Drube, Sebastian AU - Drube S AD - Institute of Immunology, Jena University Hospital, Friedrich-Schiller-University, Jena, Germany. FAU - Hartmann, Monika AU - Hartmann M AD - Department of Hematology and Oncology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. FAU - Sasca, Daniel AU - Sasca D AD - Department of Hematology and Oncology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. FAU - Brenner, Walburgis AU - Brenner W AUID- ORCID: 0000-0002-1511-6212 AD - Department of Obstetrics and Gynecology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. FAU - Najafi, Sara AU - Najafi S AD - Hopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany. AD - Clinical Cooperation Unit Pediatric Oncology (B310), German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK), Heidelberg, Germany. AD - National Center for Tumor Diseases Heidelberg, Heidelberg, Germany. FAU - Oehme, Ina AU - Oehme I AUID- ORCID: 0000-0002-0827-2356 AD - Hopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany. AD - Clinical Cooperation Unit Pediatric Oncology (B310), German Cancer Research Center (DKFZ) and German Cancer Consortium (DKTK), Heidelberg, Germany. AD - National Center for Tumor Diseases Heidelberg, Heidelberg, Germany. FAU - Hieber, Christoph AU - Hieber C AD - Department of Dermatology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. FAU - Zeyn, Yanira AU - Zeyn Y AUID- ORCID: 0000-0002-2501-8149 AD - Department of Dermatology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. FAU - Bros, Matthias AU - Bros M AD - Department of Dermatology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. FAU - Sippl, Wolfgang AU - Sippl W AUID- ORCID: 0000-0002-5985-9261 AD - Department of Medicinal Chemistry, Institute of Pharmacy, Martin-Luther-University of Halle-Wittenberg, Halle (Saale), Germany. wolfgang.sippl@pharmazie.uni-halle.de. FAU - Kramer, Oliver H AU - Kramer OH AUID- ORCID: 0000-0003-3973-045X AD - Institute of Toxicology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. okraemer@uni-mainz.de. LA - eng GR - KR2291/15-1, DFG-project number 495271833/Deutsche Forschungsgemeinschaft (German Research Foundation)/ GR - SI868/26-1, 468534282, 49527183/Deutsche Forschungsgemeinschaft (German Research Foundation)/ PT - Journal Article DEP - 20261028 PL - England TA - Leukemia JT - Leukemia JID - 8704895 SB - IM COIS- Competing interests: OHK declares the patents WO2019/034538, WO2016020369A1, and WO/2004/027418, and paid advisory work for BASF Ludwigshafen, Germany. WO2019/034538 (Synthesis, Pharmacology and use of New and Selective FMS-like tyrosine kinase 3 (FLT3) FLT3 Inhibitors) covers substance classes that are discussed in this work. The substances that are covered in these patents are not the same as those shown in the submitted manuscript. The BASF has not influenced our study, and its products are not discussed in the manuscript. Thus, there are no direct competing interests. All other authors declare that they have no conflict of interest. Ethics approval and consent to participate: Peripheral blood and bone marrow samples were obtained from AML patients with written informed consent in accordance with the Declaration of Helsinki under ethical approval REC: 17/LO/1566. Institutional Review Board Statement Zebrafish husbandry (permit number 35-9185.64/BH Hassel) and experiments (permit number 35-9185.81/G-126/15) were performed according to local animal welfare standards (Tierschutzgesetz section sign11, Abs. 1, No. 1) and in accordance with European Union animal welfare guidelines (EU Directive 2010/63/EU). All applicable national and institutional guidelines for the care and use of zebrafish were followed. Mice were bred and maintained in the Central Animal Facility of the Johannes Gutenberg-University Mainz or the Animal Research Facility of the Jena University Hospital under specific pathogen-free conditions on a standard diet according to the guidelines of the regional animal care committees. The "Principles of Laboratory Animal Care" (NIH publication no. 85-23, revised 1985) were followed. All experiments were approved by regional regulatory authorities (Landesuntersuchungsamt Rheinland-Pfalz, Koblenz, Germany or Thuringer Landesamt fur Lebensmittelsicherheit und Verbraucherschutz (TLLV), Bad Langensalza, Germany; licence twz-08-2023). Mice at 8-12 weeks of age were sacrificed for organ retrieval according to section sign 4(3) TierSchG. Consent for publication: All authors approve the manuscript and agree to this submission. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:22 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/12/14 00:00 [received] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/09/11 00:00 [revised] PHST- 2026/10/30 00:22 [entrez] AID - 10.1038/s41375-026-03168-x [pii] AID - 10.1038/s41375-026-03168-x [doi] PST - aheadofprint SO - Leukemia. 2026 Oct 28. doi: 10.1038/s41375-026-03168-x. PMID- 42957748 OWN - NLM STAT- Publisher LR - 20261030 IS - 1745-7254 (Electronic) IS - 1671-4083 (Linking) DP - 2026 Oct 30 TI - TNF signaling at the brain-heart interface: neurovascular injury and cardiovascular vulnerability in epilepsy. LID - 10.1038/s41401-026-01949-9 [doi] AB - Epilepsy is increasingly viewed as a systemic disorder in which recurrent seizures intersect with vascular, autonomic, respiratory, and cardiac processes to influence cardiovascular vulnerability. Seizure-related neuroinflammation involves multiple interacting mediator pathways, including HMGB1/TLR4-RAGE signaling, inflammasome/IL-1beta activation, IL-6/IL-6R-gp130 signaling, albumin/TGF-beta signaling following blood-brain barrier (BBB) disruption, and tumor necrosis factor-alpha (TNF-alpha)/TNF receptor (TNFR) signaling. Within this inflammatory network, TNF/TNFR signaling represents a mechanistically distinct and translationally relevant framework for examining how immune activation intersects with endothelial-barrier dysfunction, neurovascular unit injury, autonomic disturbance, inflammatory communication across neural and cardiovascular compartments, and cardiac vulnerability. This review integrates direct evidence from epilepsy studies with mechanistic insights from related experimental studies of BBB injury, myocardial ischemia-reperfusion injury, cardioprotection, and brain-heart communication. TNF biology is determined by ligand form, receptor subtype, cellular compartment, and disease context. Soluble TNF preferentially engages TNFR1-associated inflammatory and injury-amplifying pathways, whereas transmembrane TNF/TNFR2 signaling may support regulatory or reparative responses in selected settings. In epilepsy, seizure-associated inflammation, BBB dysfunction, glial activation, and autonomic imbalance may interact to increase susceptibility to arrhythmogenic stress, myocardial injury, adverse cardiac remodeling, and cardiorespiratory instability relevant to sudden unexpected death in epilepsy (SUDEP). However, epilepsy-specific evidence directly linking TNF/TNFR signaling to cardiovascular outcomes remains limited, and many mechanistic connections currently rely on converging evidence from neurovascular and cardiovascular research. Future studies should integrate compartment-resolved inflammatory profiling with autonomic, respiratory, electrocardiographic, and cardiac phenotyping to distinguish inflammatory signatures of disease severity from modifiable contributors to cardiovascular risk. Such approaches may enable phenotype-specific strategies to better understand and ultimately mitigate the brain-heart consequences of epilepsy. CI - (c) 2026. The Author(s) under exclusive licence to The Shanghai Institute of Materia Medica, Chinese Academy of Sciences and Chinese Pharmacological Society. FAU - Sha, Ao AU - Sha A AD - Department of Emergency Medicine, Institute of Disaster Medicine and Institute of Emergency Medicine, West China Hospital, Sichuan University, Chengdu, 610041, China. FAU - Wu, Yang AU - Wu Y AD - Department of Emergency Medicine, Institute of Disaster Medicine and Institute of Emergency Medicine, West China Hospital, Sichuan University, Chengdu, 610041, China. FAU - Peng, Hao-Ran AU - Peng HR AD - Department of Emergency Medicine, Institute of Disaster Medicine and Institute of Emergency Medicine, West China Hospital, Sichuan University, Chengdu, 610041, China. FAU - Jin, Bo AU - Jin B AD - Department of Emergency Medicine, Institute of Disaster Medicine and Institute of Emergency Medicine, West China Hospital, Sichuan University, Chengdu, 610041, China. FAU - He, Bin AU - He B AD - Department of Emergency Medicine, Institute of Disaster Medicine and Institute of Emergency Medicine, West China Hospital, Sichuan University, Chengdu, 610041, China. FAU - Zhao, Zhen-Kun AU - Zhao ZK AD - Department of Emergency Medicine, Institute of Disaster Medicine and Institute of Emergency Medicine, West China Hospital, Sichuan University, Chengdu, 610041, China. FAU - He, Fang AU - He F AD - Department of Emergency Medicine, Institute of Disaster Medicine and Institute of Emergency Medicine, West China Hospital, Sichuan University, Chengdu, 610041, China. FAU - Cao, Yu AU - Cao Y AD - Department of Emergency Medicine, Institute of Disaster Medicine and Institute of Emergency Medicine, West China Hospital, Sichuan University, Chengdu, 610041, China. caoyu@wchscu.cn. FAU - Gan, Lu AU - Gan L AD - Department of Emergency Medicine, Institute of Disaster Medicine and Institute of Emergency Medicine, West China Hospital, Sichuan University, Chengdu, 610041, China. ganlu@wchscu.cn. LA - eng PT - Journal Article PT - Review DEP - 20261030 PL - United States TA - Acta Pharmacol Sin JT - Acta pharmacologica Sinica JID - 100956087 SB - IM OTO - NOTNLM OT - blood-brain barrier OT - brain-heart interface OT - epilepsy OT - neurovascular unit OT - tumor necrosis factor-alpha OT - vagus nerve stimulation COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:22 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/21 00:00 [received] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/30 00:22 [entrez] AID - 10.1038/s41401-026-01949-9 [pii] AID - 10.1038/s41401-026-01949-9 [doi] PST - aheadofprint SO - Acta Pharmacol Sin. 2026 Oct 30. doi: 10.1038/s41401-026-01949-9. PMID- 42957743 OWN - NLM STAT- Publisher LR - 20261030 IS - 1476-5594 (Electronic) IS - 0950-9232 (Linking) DP - 2026 Oct 29 TI - IL-36gamma/IL-36R axis reprograms cancer-associated fibroblasts to promote OSCC invasion via ERK1/2-CCL20-mediated ECM remodeling. LID - 10.1038/s41388-026-04030-1 [doi] AB - The IL-36gamma/IL-36R signaling axis mediates cellular communication in various contexts and has recently been implicated in pro-fibrotic responses. However, its specific role in oral squamous cell carcinoma (OSCC), which is characterized by abundant cancer-associated fibroblasts (CAFs), remains elusive. Here, we investigated the contribution of IL-36gamma/IL-36R signaling to CAF function and OSCC progression. Immunohistochemical analysis of patient samples revealed that IL-36gamma was highly expressed in CAFs and that higher co-expression of IL-36gamma/IL-36R in CAFs was associated with more invasive patterns and shorter disease-free survival. By integrating tumor cell lines with patient-derived organoids (PDOs), we established three co-culture systems to model these interactions. These models revealed that IL-36gamma signaling enhanced CAF proliferation and extracellular matrix remodeling. Mechanistically, the IL-36gamma/IL-36R axis in CAFs increased ERK1/2-dependent CCL20 secretion, which in turn promoted tumor cell invasion. Pharmacological blockade of IL-36R attenuated both the IL-36gamma-induced increase in CAF abundance and the associated pro-tumorigenic CAF phenotypes in vivo. Collectively, these findings suggest that the IL-36gamma/IL-36R-ERK1/2-CCL20 signaling cascade participates in CAF-tumor interactions. Targeting IL-36R may therefore represent a promising therapeutic strategy for invasive OSCC. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature Limited. FAU - Li, Zihui AU - Li Z AUID- ORCID: 0009-0001-4315-0821 AD - Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, China. AD - Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, China. FAU - Zhang, Yi AU - Zhang Y AD - Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, China. AD - School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, China. FAU - Luo, Wenfeng AU - Luo W AD - Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, China. AD - School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, China. FAU - Jing, Yue AU - Jing Y AD - Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, China. FAU - Yu, Qiuya AU - Yu Q AD - Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, China. FAU - Song, Yuxian AU - Song Y AD - Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, China. FAU - Lu, Wanqiu AU - Lu W AD - Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, China. FAU - Xia, Shu AU - Xia S AD - Department of Oral Pathology, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, China. FAU - Chen, Xiaofang AU - Chen X AD - Drug Discovery, Shanghai Huaota Biopharmaceutical Co., Ltd., Shanghai, China. FAU - Yu, Haijia AU - Yu H AD - Drug Discovery, Shanghai Huaota Biopharmaceutical Co., Ltd., Shanghai, China. FAU - Zhu, Xi AU - Zhu X AD - Drug Discovery, Shanghai Huaota Biopharmaceutical Co., Ltd., Shanghai, China. FAU - Zhu, Xiangyang AU - Zhu X AD - Drug Discovery, Shanghai Huaota Biopharmaceutical Co., Ltd., Shanghai, China. FAU - Zhan, Yifan AU - Zhan Y AD - Drug Discovery, Shanghai Huaota Biopharmaceutical Co., Ltd., Shanghai, China. FAU - Wang, Zhiyong AU - Wang Z AUID- ORCID: 0000-0003-0563-2413 AD - Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, China. zywangkq@nju.edu.cn. FAU - Zhang, Xiaoxin AU - Zhang X AUID- ORCID: 0009-0007-1743-0989 AD - Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, China. 693216371@qq.com. FAU - Ni, Yanhong AU - Ni Y AUID- ORCID: 0000-0002-3890-3884 AD - Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Affiliated Hospital of Medical School, Institute of Stomatology, Nanjing University, Nanjing, China. Yanhong.Ni@nju.edu.cn. LA - eng GR - 82573289/National Natural Science Foundation of China (National Science Foundation of China)/ GR - 82173159/National Natural Science Foundation of China (National Science Foundation of China)/ GR - 82002865/National Natural Science Foundation of China (National Science Foundation of China)/ PT - Journal Article DEP - 20261029 PL - England TA - Oncogene JT - Oncogene JID - 8711562 SB - IM COIS- Competing interests: Authors XFC, HJY, XZ, XYZ, and YFZ were employed by Shanghai Huaota Biopharmaceutical Co., Ltd. The remaining authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:21 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/10 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/09/19 00:00 [revised] PHST- 2026/10/30 00:21 [entrez] AID - 10.1038/s41388-026-04030-1 [pii] AID - 10.1038/s41388-026-04030-1 [doi] PST - aheadofprint SO - Oncogene. 2026 Oct 29. doi: 10.1038/s41388-026-04030-1. PMID- 42957738 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261030 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 30 TI - Dynamic carbon overlayered CuCo alloy promotes syngas to higher alcohols. LID - 11239 [pii] LID - 10.1038/s41467-026-78203-7 [doi] AB - Syngas (CO/H(2)), sourced from coal, biomass, and shale gas, plays a crucial role in producing a wide range of industrially essential hydrocarbons and oxygenates through Fischer-Tropsch synthesis and its variations. Despite extensive research dating back nearly a century, transforming syngas into higher alcohols remains challenging due to issues with low selectivity and catalyst instability. Generally, more than 50% of CO in typical syngas conversions yields unwanted hydrocarbons, mainly alkanes. Herein, we develop a catalyst integrating a Mo(2)C-matrix-supported CuCo alloy with hydrophobic graphitic carbon overlayers, achieving 80% selectivity toward value-added alcohols and olefins, of which 59% are higher alcohols, while limiting alkanes to around 10%. The overlayers dynamically participate in the reaction through carbon consumption and replenishment, generating carbon-overlayer-derived CH(x) species that form an asymmetrically polarised coupling pair with CO-derived intermediates, thereby lowering the barrier for CO insertion and diverting CO from deoxygenation pathways leading to alkane and coke formation. Consequently, the yield of value-added products reaches four times that of typical benchmarks at 80% CO conversion with long-term stability. This work represents a substantial advance in higher alcohol synthesis and sheds new light on the catalytic role of surface carbon in syngas conversion. CI - (c) 2026. The Author(s). FAU - Liu, Jia AU - Liu J AD - State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China. FAU - Ying, Si-Wei AU - Ying SW AUID- ORCID: 0000-0002-9781-5585 AD - State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China. FAU - Zuo, Jiachang AU - Zuo J AUID- ORCID: 0000-0001-7976-1037 AD - State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China. FAU - Zhang, Qiuyue AU - Zhang Q AUID- ORCID: 0000-0003-0953-5880 AD - State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China. FAU - Gao, Shan AU - Gao S AD - State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China. FAU - Liang, Xuelian AU - Liang X AD - State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China. FAU - Duan, Xinping AU - Duan X AUID- ORCID: 0000-0002-9191-1437 AD - State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China. FAU - Ren, Jing AU - Ren J AD - State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, China. FAU - Zhao, Yufei AU - Zhao Y AUID- ORCID: 0000-0002-5325-8991 AD - State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, China. FAU - Chen, Mingshu AU - Chen M AUID- ORCID: 0000-0002-4923-9632 AD - State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China. FAU - Ye, Linmin AU - Ye L AD - State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China. FAU - Xie, Su-Yuan AU - Xie SY AUID- ORCID: 0000-0003-2370-9947 AD - State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China. FAU - Zheng, Jian-Wei AU - Zheng JW AUID- ORCID: 0000-0001-5027-573X AD - State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China. jwzheng@xmu.edu.cn. FAU - Yuan, Youzhu AU - Yuan Y AUID- ORCID: 0000-0003-1668-9984 AD - State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China. yzyuan@xmu.edu.cn. LA - eng PT - Journal Article DEP - 20260930 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 SB - IM COIS- Competing interests: A patent (No. 202410727261.7) relating to the findings reported in this study has been granted to Xiamen University, with Y.Z.Y., J.L., J.C.Z., L.M.Y., J.-W.Z., and X.L.L. as the inventors. The remaining authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:21 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/01/31 00:00 [received] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/30 00:21 [entrez] AID - 10.1038/s41467-026-78203-7 [pii] AID - 10.1038/s41467-026-78203-7 [doi] PST - epublish SO - Nat Commun. 2026 Sep 30;17(1):11239. doi: 10.1038/s41467-026-78203-7. PMID- 42957733 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 29 TI - A SAGA-like AP2-P-ARP1-GCN5 transcriptional complex targets pathogenesis-associated genes during the blood-stage development of Babesia gibsoni. LID - 11228 [pii] LID - 10.1038/s41467-026-78127-2 [doi] AB - Babesiosis is an increasingly recognized tick-borne zoonosis. The asexual blood stage of its causative agent, Babesia, drives pathological damages in the host and can cause severe or fatal human infections. Nevertheless, the transcriptional regulatory mechanisms that control this developmental stage remain poorly characterized. Here, we identified a novel ApiAP2 transcription factor (AP2-P; pathogenesis), which is essential in the blood-stage development of B. gibsoni. Nuclear import of AP2-P is inhibited by ivermectin, which is likely mediated via the IMPalpha/beta pathway. Importantly, AP2-P forms a SAGA-like complex with the histone acetyltransferase GCN5 and a novel AP2-P related protein 1 (ARP1). Conditional knockdown of ARP1 or GCN5 results in reduced parasite growth rates accompanied by alterations in expression profiles and chromatin accessibility of pathogenesis-related genes. ChIP-seq data indicate that the complex associates with both euchromatic regions marked by H3K9ac and heterochromatic regions marked by H3K9me3, and it also targets many pathogenic genes by recognizing specific DNA motif, including those involved in invasion, antigenic variation, drug resistance, and sexual-stage development. Notably, normal genomic distribution pattern of AP2-P likewise depends on ARP1 or GCN5. Collectively, our study suggests a SAGA-like epigenetic module responsible for pathogenesis of Babesia gibsoni during asexual blood stage. CI - (c) 2026. The Author(s). FAU - Han, Zhen AU - Han Z AD - National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, China. FAU - Zheng, Yaxin AU - Zheng Y AD - National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, China. FAU - Liu, Wei AU - Liu W AD - National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, China. FAU - Luo, Wanxin AU - Luo W AD - National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, China. FAU - Wu, Chenglong AU - Wu C AD - National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, China. FAU - Wang, Jianyu AU - Wang J AD - National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, China. FAU - Chen, Fangwei AU - Chen F AD - National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, China. FAU - He, Lan AU - He L AUID- ORCID: 0000-0002-5453-4425 AD - National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. helan@mail.hzau.edu.cn. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, China. helan@mail.hzau.edu.cn. AD - Hubei Jiangxia Laboratory, Wuhan, Hubei, 430200, China. helan@mail.hzau.edu.cn. AD - Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Wuhan, Hubei, 430070, China. helan@mail.hzau.edu.cn. FAU - Zhao, Junlong AU - Zhao J AUID- ORCID: 0000-0001-5162-4495 AD - National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. zhaojunlong@mail.hzau.edu.cn. AD - Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, China. zhaojunlong@mail.hzau.edu.cn. AD - Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Wuhan, Hubei, 430070, China. zhaojunlong@mail.hzau.edu.cn. LA - eng GR - 32273030/National Natural Science Foundation of China (National Science Foundation of China)/ GR - 31772729/National Natural Science Foundation of China (National Science Foundation of China)/ PT - Journal Article DEP - 20260929 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 RN - 0 (Protozoan Proteins) RN - EC 2.3.1.48 (p300-CBP-Associated Factor) RN - EC 2.3.1.48 (p300-CBP Transcription Factors) RN - 70288-86-7 (Ivermectin) RN - 0 (Transcription Factors) RN - EC 2.3.1.48 (Histone Acetyltransferases) SB - IM MH - *Babesia/genetics/growth & development/metabolism/pathogenicity MH - Animals MH - *Protozoan Proteins/metabolism/genetics MH - p300-CBP-Associated Factor MH - *Babesiosis/parasitology MH - *p300-CBP Transcription Factors/metabolism/genetics MH - Ivermectin/pharmacology MH - Humans MH - *Transcription Factors/metabolism/genetics MH - Histone Acetyltransferases/metabolism COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:20 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/14 00:00 [received] PHST- 2026/09/10 00:00 [accepted] PHST- 2026/10/30 00:20 [entrez] AID - 10.1038/s41467-026-78127-2 [pii] AID - 10.1038/s41467-026-78127-2 [doi] PST - epublish SO - Nat Commun. 2026 Sep 29;17(1):11228. doi: 10.1038/s41467-026-78127-2. PMID- 42957732 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 29 TI - Structural mechanisms underlying resistance to Switch-II pocket inhibitors in KRAS. LID - 11229 [pii] LID - 10.1038/s41467-026-78133-4 [doi] AB - Direct targeting of KRAS through the Switch-II pocket is an effective therapeutic strategy, but acquired resistance limits clinical benefit. We examine clinically observed resistance mutations (R68S, H95Q, Y96C, Y96D and Q99L) arising during KRAS G12C inhibitor therapy using biochemical, structural, computational and cellular approaches. Covalent and noncovalent binding measurements reveal mutation- and inhibitor-specific reductions in drug engagement across inhibitor classes. Structural analyses identify two principal resistance mechanisms: Type I, involving loss of key inhibitor contacts, and Type II, involving stabilization of pocket-occluding Switch-II conformations. Y96D exhibits both mechanisms through nucleotide-dependent non-native intramolecular interactions, whereas R68S primarily exhibits Type I resistance with modest Type II effects on pocket architecture and Switch-region dynamics. Y96C, H95Q and Q99L predominantly exhibit Type I resistance. All mutants retain RAF1 binding, whereas PI3Kalpha binding is selectively reduced by R68S and Q99L. Together, these findings provide structural insights for understanding on-target resistance to Switch-II pocket inhibitors. CI - (c) 2026. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply. FAU - Whitley, Matthew J AU - Whitley MJ AUID- ORCID: 0000-0001-8830-7514 AD - NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA. FAU - Dyba, Marcin AU - Dyba M AUID- ORCID: 0000-0001-6311-6877 AD - NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA. FAU - Chakrabarti, Mayukh AU - Chakrabarti M AUID- ORCID: 0000-0003-2653-9728 AD - NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA. FAU - Smith, Brian P AU - Smith BP AD - NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA. FAU - Chan, Albert H AU - Chan AH AD - NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA. FAU - Denson, John-Paul AU - Denson JP AD - NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA. FAU - Messing, Simon A AU - Messing SA AUID- ORCID: 0000-0001-8991-2097 AD - NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA. FAU - Lin, Ken AU - Lin K AD - BridgeBio Oncology Therapeutics, South San Francisco, CA, USA. FAU - Cornilescu, Gabriel AU - Cornilescu G AUID- ORCID: 0000-0002-1204-8904 AD - NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA. FAU - Balius, Trent E AU - Balius TE AUID- ORCID: 0000-0002-6811-4667 AD - NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA. FAU - Nissley, Dwight V AU - Nissley DV AUID- ORCID: 0000-0001-7523-116X AD - NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA. FAU - McCormick, Frank AU - McCormick F AUID- ORCID: 0000-0002-6619-7120 AD - NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA. AD - Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, CA, USA. FAU - Maciag, Anna E AU - Maciag AE AUID- ORCID: 0000-0003-2641-4212 AD - NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA. FAU - Simanshu, Dhirendra K AU - Simanshu DK AUID- ORCID: 0000-0002-9717-4618 AD - NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA. dhirendra.simanshu@nih.gov. LA - eng GR - 75N91019D00024/U.S. Department of Health & Human Services | NIH | National Cancer Institute (NCI)/ PT - Journal Article DEP - 20260929 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 RN - EC 3.6.5.2 (Proto-Oncogene Proteins p21(ras)) RN - 0 (KRAS protein, human) RN - 0 (Protein Kinase Inhibitors) SB - IM MH - *Proto-Oncogene Proteins p21(ras)/genetics/antagonists & inhibitors/chemistry/metabolism MH - Humans MH - *Drug Resistance, Neoplasm/genetics MH - Mutation MH - *Protein Kinase Inhibitors/pharmacology/chemistry MH - Protein Binding MH - Binding Sites MH - Models, Molecular MH - Protein Conformation MH - Cell Line, Tumor COIS- Competing interests: F.M. serves as a consultant for Ideaya Biosciences, Leidos Biomedical Research, Daiichi Sankyo, Amgen, OPNA-IO, and Quanta Therapeutics; has received research funding from Boehringer Ingelheim; is a consultant for and co-founder of BridgeBio Pharma, and is on the Board of Directors of BridgeBio Oncology Therapeutics (BBOT). K.L. is an employee of BBOT. D.K.S., D.V.N., A.E.M., A.H.C., M.D., and B.P.S. report receiving research funding under Collaborative Research and Development Agreements (CRADAs) between the Frederick National Laboratory for Cancer Research (FNLCR) and TheRas/BBOT, and from NCI contract 75N91019D00024. A.E.M., D.K.S., and A.H.C. are coinventors on patents related to KRAS inhibitors (BBO-8520 and BBO-11818) and receive royalties through licensing from TheRas/BBOT. D.K.S. and D.V.N. additionally report receiving research funding under a CRADA between FNLCR and Qubit Pharmaceuticals. All other authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:20 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/04 00:00 [received] PHST- 2026/08/28 00:00 [accepted] PHST- 2026/10/30 00:20 [entrez] AID - 10.1038/s41467-026-78133-4 [pii] AID - 10.1038/s41467-026-78133-4 [doi] PST - epublish SO - Nat Commun. 2026 Sep 29;17(1):11229. doi: 10.1038/s41467-026-78133-4. PMID- 42957728 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2056-676X (Electronic) IS - 2056-676X (Linking) VI - 12 IP - 1 DP - 2026 Oct 29 TI - Twin pregnancy. LID - 72 [pii] LID - 10.1038/s41572-026-00746-2 [doi] AB - Twin pregnancies occur in ~12 per 1,000 pregnancies worldwide and are associated with substantially increased maternal, fetal and neonatal morbidity and mortality compared with singleton pregnancies. Although zygosity describes whether twins arise from one fertilized egg (monozygotic) or two fertilized ova (dizygotic), clinical risk is primarily determined by chorionicity (whether twins share a placenta) and amnionicity (whether twins share an amniotic sac). Accurate early determination of chorionicity and amnionicity is therefore fundamental to counselling, risk stratification, surveillance and management, and should be established and documented consistently throughout pregnancy. Monochorionic twins are uniquely susceptible to complications arising from shared placental vascular anastomoses, including twin-twin transfusion syndrome and twin anaemia-polycythaemia sequence or unequal placental sharing in the form of selective fetal growth restriction. Preterm birth remains the leading cause of perinatal morbidity and mortality in twin pregnancies, with the risk of extreme preterm birth markedly higher than in singleton gestations. Contemporary surveillance strategies aim to identify evolving complications sufficiently early to enable timely intervention and optimize outcomes. CI - (c) 2026. Springer Nature Limited. FAU - Khalil, Asma AU - Khalil A AUID- ORCID: 0000-0003-2802-7670 AD - City St George's, University of London, London, UK. asmakhalil79@googlemail.com. AD - Fetal Medicine Unit, Department of Obstetrics and Gynaecology, St George's University Hospital NHS Foundation Trust, London, UK. asmakhalil79@googlemail.com. AD - Fetal Medicine Unit, Liverpool Women's Hospital, University of Liverpool, Liverpool, UK. asmakhalil79@googlemail.com. FAU - Craig, Jeffrey M AU - Craig JM AD - IMPACT, The Institute for Mental and Physical Health and Clinical Translation, School of Medicine, Deakin University, Geelong, Victoria, Australia. AD - Murdoch Children's Research Institute, The Royal Children's Hospital, Parkville, Victoria, Australia. AD - Department of Paediatrics, The University of Melbourne, Parkville, Victoria, Australia. FAU - Ernst, Stephanie AU - Ernst S AUID- ORCID: 0000-0002-9923-862X AD - The TAPS Support Foundation, Almere, The Netherlands. FAU - Lewi, Liesbeth AU - Lewi L AUID- ORCID: 0000-0002-9884-5778 AD - Department of Development and Regeneration, KU Leuven, Leuven, Belgium. AD - Department of Obstetrics and Gynecology, University Hospitals Leuven, Leuven, Belgium. FAU - Lopriore, Enrico AU - Lopriore E AD - Department of Pediatrics, Division of Neonatology, Willem Alexander Children's Hospital, Leiden University Medical Center, Leiden, The Netherlands. FAU - Melamed, Nir AU - Melamed N AD - Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Sunnybrook Health Sciences Centre, University of Toronto, Toronto, Ontario, Canada. FAU - Monden, Christiaan AU - Monden C AD - Nuffield College & Department of Sociology, University of Oxford, Oxford, UK. FAU - Romero, Roberto AU - Romero R AD - Pregnancy Research Branch, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, United States Department of Health and Human Services, Bethesda, MA, USA. AD - Department of Obstetrics and Gynecology, University of Michigan, Ann Arbor, MI, USA. AD - Department of Epidemiology and Biostatistics, Michigan State University, East Lansing, MI, USA. AD - Department of Obstetrics and Gynecology, George Washington University, School of Medicine and Health Sciences, Washington, DC, USA. FAU - Rolnik, Daniel L AU - Rolnik DL AUID- ORCID: 0000-0002-2263-3592 AD - Department of Obstetrics and Gynaecology, Monash University, Melbourne, Victoria, Australia. AD - Monash Ultrasound for Women, Melbourne, Victoria, Australia. FAU - Segal, Nancy L AU - Segal NL AD - California State University, Fullerton, Psychology Department, Fullerton, CA, USA. FAU - Sorrenti, Sara AU - Sorrenti S AD - Fetal Medicine Unit, Liverpool Women's Hospital, University of Liverpool, Liverpool, UK. FAU - Ville, Yves AU - Ville Y AD - Hospital Necker-Enfants Malades, AP-HP, Universite Paris Cite, Paris, France. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - Nat Rev Dis Primers JT - Nature reviews. Disease primers JID - 101672103 SB - IM MH - Humans MH - Female MH - Pregnancy MH - *Pregnancy, Twin/physiology MH - Premature Birth/epidemiology MH - Fetal Growth Retardation MH - Fetofetal Transfusion/physiopathology MH - Placenta/physiopathology MH - Infant, Newborn COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:20 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/30 00:20 [entrez] AID - 10.1038/s41572-026-00746-2 [pii] AID - 10.1038/s41572-026-00746-2 [doi] PST - epublish SO - Nat Rev Dis Primers. 2026 Oct 29;12(1):72. doi: 10.1038/s41572-026-00746-2. PMID- 42957727 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261030 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Oct 1 TI - Head-to-tail assembled borphyrins for solid-state lithium extraction. LID - 11270 [pii] LID - 10.1038/s41467-026-78080-0 [doi] AB - Lithium is a critical mineral for advancing greener technologies. Extraction of lithium ions (Li(+)) from sea salt, minerals, and waste is important, but has been hindered by the lack of extraction methods that offer both high selectivity and strong lithium affinity. Here, we report a dioxaborole-linked, head-to-tail assembled macrocycle - termed borphyrin - featuring a rigid cavity with a 16-crown-4-like configuration. This molecule exhibits strong affinity for lithium and high selectivity over competing cations under dry solid-liquid extraction conditions, owing to its narrow binding cavity and planar macrocyclic core. Borphyrin enabled the extraction of lithium from crystalline solids of ether-insoluble salts, as well as selective extraction of Li(+) from dried mixed-salt matrices containing alkali, alkaline earth and transition-metal salts. These results establish borphyrin as a potential molecular platform for selective lithium recognition and solid-liquid lithium extraction. CI - (c) 2026. The Author(s). FAU - Hamzehpoor, Ehsan AU - Hamzehpoor E AUID- ORCID: 0000-0002-5427-9444 AD - Department of Chemistry, University of British Columbia, Vancouver, BC, Canada. AD - Department of Chemistry, University of Montreal, Montreal, QC, Canada. FAU - Ota, Seiya AU - Ota S AUID- ORCID: 0000-0002-6003-4793 AD - Department of Chemistry, University of British Columbia, Vancouver, BC, Canada. FAU - Chowdhury, Suprio AU - Chowdhury S AD - Department of Chemistry, University of British Columbia, Vancouver, BC, Canada. FAU - Li, Zongzhe AU - Li Z AUID- ORCID: 0000-0002-5213-740X AD - Department of Chemistry, University of British Columbia, Vancouver, BC, Canada. FAU - Yeung, Ashley AU - Yeung A AD - Department of Chemistry, University of British Columbia, Vancouver, BC, Canada. FAU - Parcon, Rhianna A AU - Parcon RA AUID- ORCID: 0009-0001-4459-968X AD - Department of Chemistry, University of British Columbia, Vancouver, BC, Canada. FAU - Kandel, Raksha AU - Kandel R AD - Department of Chemistry, University of British Columbia, Vancouver, BC, Canada. FAU - Patrick, Brian O AU - Patrick BO AD - Department of Chemistry, University of British Columbia, Vancouver, BC, Canada. FAU - MacLachlan, Mark J AU - MacLachlan MJ AUID- ORCID: 0000-0002-3546-7132 AD - Department of Chemistry, University of British Columbia, Vancouver, BC, Canada. mmaclach@chem.ubc.ca. AD - Stewart Blusson Quantum Matter Institute, University of British Columbia, Vancouver, BC, Canada. mmaclach@chem.ubc.ca. LA - eng GR - CRC-2021-00050/Canada Research Chairs (Chaires de recherche du Canada)/ GR - Banting Postdoctoral Fellowship/Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (Conseil de Recherches en Sciences Naturelles et en Genie du Canada)/ GR - 4YF Scholarship/University of British Columbia (UBC)/ GR - 4YF Scholarship/University of British Columbia (UBC)/ GR - 4YF Scholarship/University of British Columbia (UBC)/ PT - Journal Article DEP - 20261001 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 SB - IM COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:20 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/27 00:00 [received] PHST- 2026/09/12 00:00 [accepted] PHST- 2026/10/30 00:20 [entrez] AID - 10.1038/s41467-026-78080-0 [pii] AID - 10.1038/s41467-026-78080-0 [doi] PST - epublish SO - Nat Commun. 2026 Oct 1;17(1):11270. doi: 10.1038/s41467-026-78080-0. PMID- 42957722 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 30 TI - RuPHOTACs provide photocontrol over protein degradation with optimized properties for biological applications. LID - 11257 [pii] LID - 10.1038/s41467-026-78110-x [doi] AB - PROteolysis TArgeting Chimeras (PROTACs) are bifunctional molecules that catalyze degradation of selected proteins by inducing protein:protein interactions (PPIs) between E3 ubiquitin ligases and the protein of interest. A critical limitation is undesired effects in untargeted tissues, necessitating approaches to impose spatiotemporal control over PROTAC function. Here we present Ru(II) photocages that can be released with low energy light, providing triggered PROTAC activity on demand. The systems, termed Ruthenium-based PHOToActivated Chimeras (RuPHOTACs), were validated by targeting bromodomain-containing proteins, which act as crucial epigenetic regulators, and also strongly reduced levels of c-MYC and PIM1. RuPHOTACs demonstrate that the incorporation of metal components within organic PROTACs confers multiple advantages for light-controlled systems for chemical biology applications, including the highest selectivity for activation in the light vs. the dark, improved potency against the target proteins of interest, and increased efficacy in vivo using low energy red light. To efficiently monitor protein degradation via optical means, a strategy was implemented by creating a fusion of a photoconvertible protein, Dendra2, with the proteins of interest. This bifunctional reporter system for live cell analysis decouples protein degradation efficiency and rates from signals arising from new protein production, and is superior to prior reporter systems described for PROTACs because it directly measures the true degradation rate of the target protein in intact, viable cells while simultaneously tracking newly synthesized protein without perturbing translation, proteostasis, or cell health. CI - (c) 2026. The Author(s). FAU - Havrylyuk, Dmytro AU - Havrylyuk D AD - Department of Chemistry, North Carolina State University, Raleigh, NC, USA. FAU - LaMore, Ainsley P AU - LaMore AP AD - Department of Chemistry, North Carolina State University, Raleigh, NC, USA. FAU - Al-Hamaly, Majd A AU - Al-Hamaly MA AD - Pharmacology and Nutritional Sciences, University of Kentucky, Lexington, KY, USA. FAU - Blackburn, Jessica S AU - Blackburn JS AD - Department of Cellular and Molecular Biochemistry, University of Kentucky, Lexington, KY, USA. FAU - Heidary, David K AU - Heidary DK AD - Department of Chemistry, North Carolina State University, Raleigh, NC, USA. dheidar@ncsu.edu. FAU - Glazer, Edith C AU - Glazer EC AUID- ORCID: 0000-0002-0190-7742 AD - Department of Chemistry, North Carolina State University, Raleigh, NC, USA. eglazer@ncsu.edu. LA - eng GR - R01GM162746/U.S. Department of Health & Human Services | National Institutes of Health (NIH)/ PT - Journal Article DEP - 20260930 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 RN - 0 (Proteolysis Targeting Chimera) RN - 7UI0TKC3U5 (Ruthenium) RN - EC 2.3.2.27 (Ubiquitin-Protein Ligases) SB - IM MH - Proteolysis Targeting Chimera MH - *Proteolysis/radiation effects MH - Humans MH - *Ruthenium/chemistry MH - Ubiquitin-Protein Ligases/metabolism MH - Animals MH - Light MH - Red Light COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:19 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/01/19 00:00 [received] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/30 00:19 [entrez] AID - 10.1038/s41467-026-78110-x [pii] AID - 10.1038/s41467-026-78110-x [doi] PST - epublish SO - Nat Commun. 2026 Sep 30;17(1):11257. doi: 10.1038/s41467-026-78110-x. PMID- 42957717 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 30 TI - Repurposing Cas12a2-mediated abortive infection for efficient and low-toxicity microbial genome editing. LID - 11264 [pii] LID - 10.1038/s41467-026-78205-5 [doi] AB - CRISPR-Cas systems have revolutionized microbial genome engineering, yet counter-selection strategies typically rely on targeted DNA cleavage, where the severe cytotoxicity of double-strand breaks restricts transformation efficiency and clonal recovery. Here, we present an innovative strategy, SuCas12a2-Universal Recombination Editing (SURE), that circumvents these limitations by repurposing the RNA-targeting CRISPR-Cas12a2 system. Instead of directly attacking the genome, SURE utilizes Cas12a2's RNA-triggered collateral activity to induce an abortive infection-like state, providing a potent yet recoverable selective pressure. This strategy increases transformation efficiency by up to 1,000-fold compared to Cas9- or Cas12-based systems while achieving over 90% editing efficiency for gene knockout, insertion, and replacement in Escherichia coli MG1655. We validate the SURE platform's broad utility by extending it to diverse species, including Bacillus subtilis BSZR and Shewanella oneidensis MR-1. Mechanistically, we identify collateral double-stranded DNA degradation as the decisive factor for driving efficient selection. Finally, we apply this system to engineer E. coli MG1655 for efficient assimilation of the plastic monomer ethylene glycol, demonstrating its practical utility. Collectively, this work establishes a universal and robust RNA-guided editing framework that resolves the toxicity-efficiency trade-off inherent to the conventional DNA-targeting methods. CI - (c) 2026. The Author(s). FAU - Wang, Hao-Da AU - Wang HD AUID- ORCID: 0009-0000-6333-3257 AD - State Key Laboratory of Advanced Environmental Technology, School of Environment, University of Science and Technology of China, Hefei, China. FAU - Hu, Lan AU - Hu L AD - School of Life Sciences, University of Science and Technology of China, Hefei, China. FAU - Han, Jing AU - Han J AUID- ORCID: 0009-0000-0976-307X AD - State Key Laboratory of Advanced Environmental Technology, School of Environment, University of Science and Technology of China, Hefei, China. FAU - Cheng, Zhou-Hua AU - Cheng ZH AUID- ORCID: 0000-0001-7122-2661 AD - State Key Laboratory of Advanced Environmental Technology, School of Environment, University of Science and Technology of China, Hefei, China. zhc121@ustc.edu.cn. FAU - Yu, Han-Qing AU - Yu HQ AUID- ORCID: 0000-0001-5247-6244 AD - State Key Laboratory of Advanced Environmental Technology, School of Environment, University of Science and Technology of China, Hefei, China. AD - School of Life Sciences, University of Science and Technology of China, Hefei, China. FAU - Liu, Dong-Feng AU - Liu DF AUID- ORCID: 0000-0002-5954-6476 AD - State Key Laboratory of Advanced Environmental Technology, School of Environment, University of Science and Technology of China, Hefei, China. dfl@ustc.edu.cn. AD - School of Life Sciences, University of Science and Technology of China, Hefei, China. dfl@ustc.edu.cn. LA - eng GR - 52322002/National Natural Science Foundation of China (National Science Foundation of China)/ PT - Journal Article DEP - 20260930 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 RN - 0 (CRISPR-Associated Proteins) RN - 0 (Bacterial Proteins) RN - 0 (RNA, Guide, CRISPR-Cas Systems) RN - EC 3.1.- (Endodeoxyribonucleases) RN - EC 3.1.- (Cas12a protein) RN - Shewanella oneidensis SB - IM MH - Escherichia coli/genetics/metabolism MH - *Gene Editing/methods MH - *CRISPR-Cas Systems/genetics MH - Bacillus subtilis/genetics MH - Shewanella/genetics MH - *Genome, Bacterial MH - *CRISPR-Associated Proteins/genetics/metabolism MH - *Bacterial Proteins/genetics/metabolism MH - RNA, Guide, CRISPR-Cas Systems/genetics MH - *Endodeoxyribonucleases/genetics/metabolism COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:19 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/03 00:00 [received] PHST- 2026/09/14 00:00 [accepted] PHST- 2026/10/30 00:19 [entrez] AID - 10.1038/s41467-026-78205-5 [pii] AID - 10.1038/s41467-026-78205-5 [doi] PST - epublish SO - Nat Commun. 2026 Sep 30;17(1):11264. doi: 10.1038/s41467-026-78205-5. PMID- 42957714 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Oct 29 TI - Widespread selection of antibiotic resistance within hospital sewers. LID - 11162 [pii] LID - 10.1038/s41467-026-78836-8 [doi] AB - Environmental microbiomes are sources of diverse antibiotic resistance determinants that can be mobilized and transferred to human pathogens under a selection pressure from antibiotics. Hospital sewers contain more antibiotics than most environments and are thus increasingly recognised as potential arenas for resistance evolution. However, it is unknown if resistance selection occurs there and what the drivers are. Here we show selection of antibiotic-resistant bacteria within Swedish hospital sewers. Evidence include; consistent selection of multiple resistances in Escherichia coli exposed to filtered wastewater from ten hospitals, but not to corresponding municipal wastewaters; in-situ enrichment of resistant over sensitive E. coli from wastewater to sewer biofilms at hospitals, but not at municipal sites; and higher resistance in bacteria collected from hospital sewers than from municipal sites as assessed by culturing and metagenomics. Evidence for drivers includes complete rescue of sensitive E. coli by addition of resistance genes to fluoroquinolones and beta-lactams, and cellular effects typical for beta-lactams. Measured concentrations by liquid-chromatography-tandem-mass-spectrometry compared to selective thresholds identify ciprofloxacin and cefotaxime as principal selective agents. Antibiotic concentrations and use data allow extrapolation of risks beyond Sweden. Given the absence of detectable selection at municipal sites, mitigations of risks in hospital sewers should be prioritized. CI - (c) 2026. The Author(s). FAU - Gray, Declan A AU - Gray DA AD - Centre for Antibiotic Resistance Research in Gothenburg (CARe), University of Gothenburg, Gothenburg, Sweden. AD - Department of Infectious Diseases, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. FAU - Nhung, Nguyen Thi AU - Nhung NT AD - Centre for Antibiotic Resistance Research in Gothenburg (CARe), University of Gothenburg, Gothenburg, Sweden. AD - Department of Infectious Diseases, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. FAU - Coertze, Roelof Dirk AU - Coertze RD AD - Centre for Antibiotic Resistance Research in Gothenburg (CARe), University of Gothenburg, Gothenburg, Sweden. AD - Department of Infectious Diseases, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. FAU - Ekwanzala, Mutshiene Deogratias AU - Ekwanzala MD AUID- ORCID: 0000-0001-6881-1262 AD - Centre for Antibiotic Resistance Research in Gothenburg (CARe), University of Gothenburg, Gothenburg, Sweden. AD - Department of Infectious Diseases, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. FAU - Waters, Noel AU - Waters N AUID- ORCID: 0009-0008-0538-3238 AD - Centre for Antibiotic Resistance Research in Gothenburg (CARe), University of Gothenburg, Gothenburg, Sweden. AD - Department of Infectious Diseases, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. FAU - Jarhult, Josef D AU - Jarhult JD AUID- ORCID: 0000-0002-7075-1059 AD - Department of Medical Sciences, Zoonosis Science Center, Uppsala University, Uppsala, Sweden. FAU - Wenzel, Michaela AU - Wenzel M AUID- ORCID: 0000-0001-9969-6113 AD - Centre for Antibiotic Resistance Research in Gothenburg (CARe), University of Gothenburg, Gothenburg, Sweden. AD - Department of Life Sciences, Division of Molecular Bioscience, Chalmers University of Technology, Gothenburg, Sweden. FAU - Ahren, Christina AU - Ahren C AD - Centre for Antibiotic Resistance Research in Gothenburg (CARe), University of Gothenburg, Gothenburg, Sweden. AD - Department of Infectious Diseases, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. AD - Swedish Strategic Program Against Antimicrobial Resistance (Strama), Region Vastra Gotaland, Gothenburg, Sweden. FAU - Fick, Jerker AU - Fick J AUID- ORCID: 0000-0002-3949-7371 AD - Department of Chemistry, Umea University, Umea, Sweden. FAU - Flach, Carl-Fredrik AU - Flach CF AUID- ORCID: 0000-0002-4101-5095 AD - Centre for Antibiotic Resistance Research in Gothenburg (CARe), University of Gothenburg, Gothenburg, Sweden. AD - Department of Infectious Diseases, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. FAU - Larsson, D G Joakim AU - Larsson DGJ AUID- ORCID: 0000-0002-5496-0328 AD - Centre for Antibiotic Resistance Research in Gothenburg (CARe), University of Gothenburg, Gothenburg, Sweden. joakim.larsson@fysiologi.gu.se. AD - Department of Infectious Diseases, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden. joakim.larsson@fysiologi.gu.se. LA - eng GR - 2018-05771/Vetenskapsradet (Swedish Research Council)/ GR - 2021-00949/Svenska Forskningsradet Formas (Swedish Research Council Formas)/ GR - NA/Adlerbertska Stiftelserna (Adlerbertska Foundations)/ PT - Journal Article DEP - 20261029 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 RN - 0 (Anti-Bacterial Agents) RN - 5E8K9I0O4U (Ciprofloxacin) RN - 0 (Sewage) RN - 0 (Wastewater) RN - N2GI8B1GK7 (Cefotaxime) RN - 0 (Fluoroquinolones) RN - 0 (beta-Lactams) SB - IM MH - *Escherichia coli/drug effects/genetics/isolation & purification MH - *Anti-Bacterial Agents/pharmacology/analysis MH - Sweden MH - Ciprofloxacin/pharmacology MH - Hospitals MH - *Sewage/microbiology MH - Biofilms/drug effects MH - Humans MH - Wastewater/microbiology MH - Microbial Sensitivity Tests MH - Cefotaxime/pharmacology MH - *Drug Resistance, Bacterial/genetics MH - Fluoroquinolones/pharmacology MH - *Selection, Genetic MH - beta-Lactams/pharmacology COIS- Competing interests: D.G.J.L., D.A.G., and M.D.E. have filed a patent application on a method to treat antibiotic-contaminated wastewater. With that exception, all authors declare that they have no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:19 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/27 00:00 [received] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/30 00:19 [entrez] AID - 10.1038/s41467-026-78836-8 [pii] AID - 10.1038/s41467-026-78836-8 [doi] PST - epublish SO - Nat Commun. 2026 Oct 29;17(1):11162. doi: 10.1038/s41467-026-78836-8. PMID- 42957706 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261030 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Oct 1 TI - Nationwide sustainability assessment of PFAS treatment in U.S. water utilities. LID - 11276 [pii] LID - 10.1038/s41467-026-77509-w [doi] AB - Per- and polyfluoroalkyl substances (PFAS) pose an unprecedented challenge for US drinking water systems as new national regulations mandate their removal. While granular activated carbon (GAC) and ion exchange (IX) resins are widely applied, their economic and environmental sustainability under diverse real-world conditions remains uncertain. Here we leverage a quantitative sustainable design framework (integrated techno-economic analysis and life cycle assessment) to evaluate GAC and IX performance across utilities nationwide. Through uncertainty and sensitivity analyses, we identify utility size, water types (e.g., groundwater and surface water), and PFAS chain length as the dominant drivers of cost and environmental impacts. IX consistently shows lower global warming potential than GAC, though treatment of short-chain PFAS significantly increases costs. Water quality, especially total organic carbon, affects treatment performance and can change cost and environmental impacts for GAC. Results highlight disproportionate burdens on small and rural systems and the trade-offs inherent in technology selection. This work provides actionable insight for utilities and policymakers, enabling more equitable and sustainable strategies for meeting emerging PFAS standards. CI - (c) 2026. The Author(s). FAU - Tushar, Md Moshiur Rahman AU - Tushar MMR AUID- ORCID: 0009-0006-6693-2793 AD - Department of Civil Engineering and Construction, Georgia Southern University, Statesboro, Georgia, USA. FAU - Pushan, Zaki Alam AU - Pushan ZA AD - Department of Civil and Environmental Engineering, University of Nebraska - Lincoln, Lincoln, NE, USA. FAU - Page, Christopher Sutton AU - Page CS AD - Department of Computer Science, Georgia Southern University, Statesboro, Georgia, USA. FAU - Aich, Nirupam AU - Aich N AUID- ORCID: 0000-0003-1896-8127 AD - Department of Civil and Environmental Engineering, University of Nebraska - Lincoln, Lincoln, NE, USA. nirupam.aich@unl.edu. FAU - Rowles, Lewis Stetson AU - Rowles LS AUID- ORCID: 0000-0002-3489-9179 AD - Department of Civil Engineering and Construction, Georgia Southern University, Statesboro, Georgia, USA. lrowles@georgiasouthern.edu. LA - eng GR - 2331082/National Science Foundation (NSF)/ PT - Journal Article DEP - 20261001 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 SB - IM COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:18 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/04/13 00:00 [received] PHST- 2026/08/26 00:00 [accepted] PHST- 2026/10/30 00:18 [entrez] AID - 10.1038/s41467-026-77509-w [pii] AID - 10.1038/s41467-026-77509-w [doi] PST - epublish SO - Nat Commun. 2026 Oct 1;17(1):11276. doi: 10.1038/s41467-026-77509-w. PMID- 42957703 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Oct 1 TI - Amygdala beta bursts modulate hippocampal neuronal dynamics during human emotional processing. LID - 11272 [pii] LID - 10.1038/s41467-026-77952-9 [doi] AB - The amygdala and hippocampus are key structures in emotional processing, yet the transient neural dynamics coordinating their interaction remain poorly understood. We simultaneously recorded single-neuron activity and local field potentials from these regions in epilepsy patients performing an emotional image-rating task. Neurons in both regions exhibited firing rate changes predictive of subjective emotional valence. While conventional spectral analysis revealed no valence-specific patterns, beta bursts (13-30 Hz)-transient, high-power events-were selectively modified by task features. In both regions, beta bursts were associated with increased gamma amplitude and enhanced phase coherence, with beta-gamma phase-amplitude coupling capturing emotion-related dynamics. Critically, amygdala beta bursts were associated with valence-dependent, bidirectional modulation of hippocampal interneuron firing - decreased during very unpleasant/highest arousal processing and increased during high-arousal very pleasant processing - while putative pyramidal-cell firing did not change significantly. Hippocampal bursts showed no reciprocal influence. These findings suggest that beta bursts may represent a temporally precise neural correlate of emotional appraisal and a potential target for neuromodulation in mood disorders. CI - (c) 2026. The Author(s). FAU - Zhou, Yajun AU - Zhou Y AUID- ORCID: 0000-0003-3044-4959 AD - Department of Neurosurgery, Yale University, New Haven, CT, USA. FAU - Huang, Yuqing AU - Huang Y AUID- ORCID: 0000-0001-7955-824X AD - Department of Neurosurgery, Yale University, New Haven, CT, USA. FAU - Pittaro, Giana F AU - Pittaro GF AD - Department of Neurosurgery, Yale University, New Haven, CT, USA. FAU - Zareian, Behzad AU - Zareian B AD - Department of Neurosurgery, Yale University, New Haven, CT, USA. FAU - Aljishi, Ayman AU - Aljishi A AD - Department of Neurosurgery, Yale University, New Haven, CT, USA. FAU - Toro, Alondra P AU - Toro AP AD - Department of Neurosurgery, Yale University, New Haven, CT, USA. FAU - Zibly, Zion AU - Zibly Z AD - Department of Neurosurgery, Yale University, New Haven, CT, USA. FAU - Obaid, Sami AU - Obaid S AD - Department of Neurosurgery, Yale University, New Haven, CT, USA. FAU - Sivaraju, Adithya AU - Sivaraju A AUID- ORCID: 0000-0002-6027-9205 AD - Department of Neurology, Yale University, New Haven, CT, USA. FAU - Harpaz-Rotem, Ilan AU - Harpaz-Rotem I AUID- ORCID: 0000-0002-6066-9406 AD - Department of Psychiatry, Yale University, New Haven, CT, USA. AD - Wu Tsai Institute, Yale University, New Haven, CT, USA. FAU - Kaye, Alfred AU - Kaye A AUID- ORCID: 0000-0002-3153-1221 AD - Department of Psychiatry, Yale University, New Haven, CT, USA. AD - Wu Tsai Institute, Yale University, New Haven, CT, USA. AD - Clinical Neurosciences Division, VA National Center for PTSD, West Haven, CT, USA. FAU - Krystal, John AU - Krystal J AUID- ORCID: 0000-0001-6952-1726 AD - Department of Psychiatry, Yale University, New Haven, CT, USA. AD - Wu Tsai Institute, Yale University, New Haven, CT, USA. AD - Clinical Neurosciences Division, VA National Center for PTSD, West Haven, CT, USA. AD - Department of Neuroscience, Yale University, New Haven, CT, USA. AD - Department of Psychology, Yale University, New Haven, CT, USA. FAU - Sheth, Kevin AU - Sheth K AUID- ORCID: 0000-0003-2003-5473 AD - Department of Neurology, Yale University, New Haven, CT, USA. FAU - Gu, Xiaosi AU - Gu X AUID- ORCID: 0000-0002-9373-987X AD - Department of Psychiatry, Yale University, New Haven, CT, USA. FAU - Pittenger, Christopher AU - Pittenger C AUID- ORCID: 0000-0003-2117-9321 AD - Department of Psychiatry, Yale University, New Haven, CT, USA. AD - Wu Tsai Institute, Yale University, New Haven, CT, USA. AD - Department of Psychology, Yale University, New Haven, CT, USA. AD - Center for Brain and Mind Health, Yale University, New Haven, CT, USA. FAU - Damisah, Eyiyemisi C AU - Damisah EC AUID- ORCID: 0000-0001-8854-1335 AD - Department of Neurosurgery, Yale University, New Haven, CT, USA. eyiyemisi.damisah@yale.edu. AD - Wu Tsai Institute, Yale University, New Haven, CT, USA. eyiyemisi.damisah@yale.edu. AD - Department of Neuroscience, Yale University, New Haven, CT, USA. eyiyemisi.damisah@yale.edu. AD - Center for Brain and Mind Health, Yale University, New Haven, CT, USA. eyiyemisi.damisah@yale.edu. LA - eng GR - R01MH138291/Foundation for the National Institutes of Health (Foundation for the National Institutes of Health, Inc.)/ GR - 310142/Z/24/Z/Wellcome Trust (Wellcome)/ PT - Journal Article DEP - 20261001 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 SB - IM MH - Humans MH - *Hippocampus/physiology/cytology MH - *Amygdala/physiology MH - *Emotions/physiology MH - Male MH - Female MH - *Neurons/physiology MH - Adult MH - *Beta Rhythm/physiology MH - Local Field Potential Measurement MH - Young Adult MH - Action Potentials/physiology MH - Arousal/physiology MH - Epilepsy/physiopathology COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:18 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/09/18 00:00 [received] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/30 00:18 [entrez] AID - 10.1038/s41467-026-77952-9 [pii] AID - 10.1038/s41467-026-77952-9 [doi] PST - epublish SO - Nat Commun. 2026 Oct 1;17(1):11272. doi: 10.1038/s41467-026-77952-9. PMID- 42957697 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 29 TI - Chlorophyll as a sustainable crosslinking strategy for engineering highly compressible hydrogels. LID - 11219 [pii] LID - 10.1038/s41467-026-77673-z [doi] AB - Hydrogels are promising candidates for tissue repair. Non-invasive delivery of hydrogel patches involving high strains and deformations requires mechanical robustness and complex material design. Here we show chlorophyll, a sustainable plant-derived small molecule, functions as a universal physical crosslinker to significantly enhance hydrogels' compressibility and resilience. Chlorophylls self-assemble in aqueous pre-polymer solution via hydrophobic interactions, leading to the nano-aggregations, acting as physical crosslinkers and anchoring points within hydrogels' matrix. Additionally, the solvent exchange (SE) process promotes macroporous structure formation for high compressibility and resilience. With chlorophyll, the maximum compressive strains of gelatin methacrylate (GC) hydrogels increased from ~43% to >/=90%, allowing injection under 90.4% deformation while preserving structural integrity. This strategy enabled the fabrication of a single 36-mm diameter injectable conductive polypyrrole-deposited GC (GCP) cardiac patch for myocardial repair in minipigs. Moreover, chlorophyll functions as an intrinsic antioxidant reservoir, and improved ejection fraction (EF) by 11 % in porcine infarcted hearts. CI - (c) 2026. The Author(s). FAU - Xu, Kaige AU - Xu K AUID- ORCID: 0000-0002-2200-1499 AD - School of Medicine & Nursing and First Affiliated Hospital, Huzhou University, Huzhou, China. AD - Department of Neurology, Institute of Neuroscience, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, China. AD - Department of Mechanical Engineering, University of Manitoba, Winnipeg, MB, Canada. FAU - Zhao, Chaoran AU - Zhao C AD - Department of Neurology, Institute of Neuroscience, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, China. AD - Department of Anatomy, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, China. FAU - Liu, Yuqing AU - Liu Y AUID- ORCID: 0000-0002-6324-1296 AD - Department of Mechanical Engineering, University of Manitoba, Winnipeg, MB, Canada. FAU - Liu, Junjie AU - Liu J AD - Biomaterials Research Center, School of Biomedical Engineering, Southern Medical University, Guangzhou, China. FAU - Xing, Xianglong AU - Xing X AD - Department of Mechanical Engineering, University of Manitoba, Winnipeg, MB, Canada. FAU - Ojo, Oluwaloba Wisdom AU - Ojo OW AD - Department of Biological Science, Southern Methodist University, Dallas, TX, USA. FAU - Wu, Mingsong AU - Wu M AD - School of Medicine & Nursing and First Affiliated Hospital, Huzhou University, Huzhou, China. FAU - Wang, Quan AU - Wang Q AD - School of Civil Engineering, Shantou University, Shantou, China. FAU - Xing, Malcolm M Q AU - Xing MMQ AUID- ORCID: 0000-0002-3547-0462 AD - Department of Mechanical Engineering, University of Manitoba, Winnipeg, MB, Canada. malcolm.xing@umanitoba.ca. FAU - Wang, Leyu AU - Wang L AUID- ORCID: 0000-0002-6547-1490 AD - Department of Neurology, Institute of Neuroscience, Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and the Ministry of Education of China, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou, China. wangleyu889@163.com. AD - Department of Anatomy, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, China. wangleyu889@163.com. LA - eng PT - Journal Article DEP - 20260929 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 RN - 0 (Hydrogels) RN - 1406-65-1 (Chlorophyll) RN - 9000-70-8 (Gelatin) RN - 0 (Cross-Linking Reagents) RN - 0 (Methacrylates) SB - IM MH - *Hydrogels/chemistry MH - Animals MH - *Chlorophyll/chemistry MH - Gelatin/chemistry MH - Swine MH - *Cross-Linking Reagents/chemistry MH - *Tissue Engineering/methods MH - Hydrophobic and Hydrophilic Interactions MH - Methacrylates/chemistry COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:18 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/10/31 00:00 [received] PHST- 2026/08/19 00:00 [accepted] PHST- 2026/10/30 00:18 [entrez] AID - 10.1038/s41467-026-77673-z [pii] AID - 10.1038/s41467-026-77673-z [doi] PST - epublish SO - Nat Commun. 2026 Sep 29;17(1):11219. doi: 10.1038/s41467-026-77673-z. PMID- 42957695 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261030 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 29 TI - Hierarchical quasiparticle dynamics in antiferromagnets revealed by time- and momentum-resolved X-ray scattering. LID - 11222 [pii] LID - 10.1038/s41467-026-77964-5 [doi] AB - Energy flows among coupled subsystems are essential for ultrafast dynamics and high-speed technologies. In magnetic materials, spin fluctuations -magnons- mediate these flows in ultrafast magnetism. Yet momentum-resolved access to low-energy magnons governing the microscopic dynamics has been lacking. Using time-resolved resonant diffuse scattering alongside complementary time-resolved X-ray techniques and quantum-kinetic simulations, we unveil the hierarchical energy pathways among correlated systems in the photoexcited antiferromagnet CuO. Above-bandgap excitation triggers near-instantaneous spin disorder, generating non-thermal magnons throughout reciprocal space within femtoseconds. Real-time momentum-resolved tracking reveals picosecond magnon quasi-thermalization, followed by nanosecond recovery via momentum-selective magnon-phonon scattering. The quasiparticle dispersion mismatch creates recovery bottlenecks that control non-equilibrium lifetimes. This microscopic framework transcends phenomenological models and generalizes across antiferromagnetic insulators, establishing design principles for ultrafast control of antiferromagnetic order. CI - (c) 2026. The Author(s). FAU - Romaguera, Arnau AU - Romaguera A AUID- ORCID: 0000-0001-9014-1533 AD - PSI Center for Photon Science, Paul Scherrer Institute, Villigen-PSI, Switzerland. AD - ESRF- The European Synchrotron, 71 Avenue des Martyrs, CS40220, Grenoble, Cedex, France. FAU - Skoropata, Elizabeth AU - Skoropata E AUID- ORCID: 0000-0002-8774-1594 AD - PSI Center for Photon Science, Paul Scherrer Institute, Villigen-PSI, Switzerland. FAU - Yen, Yun AU - Yen Y AD - PSI Center for Scientific Computing, Theory and Data, Paul Scherrer Institute, Villigen-PSI, Switzerland. AD - Ecole Polytechnique Federale de Lausanne (EPFL), Lausanne, Switzerland. AD - Institute for Theoretical Physics and Bremen Center for Computational Materials Science, University of Bremen, Bremen, Germany. FAU - Liu, Biaolong AU - Liu B AD - PSI Center for Photon Science, Paul Scherrer Institute, Villigen-PSI, Switzerland. FAU - Nag, Abhishek AU - Nag A AUID- ORCID: 0000-0002-1394-5105 AD - PSI Center for Photon Science, Paul Scherrer Institute, Villigen-PSI, Switzerland. AD - Department of Physics, Indian Institute of Technology, Roorkee, Uttarakhand, India. FAU - Huang, Shih-Wen AU - Huang SW AD - PSI Center for Photon Science, Paul Scherrer Institute, Villigen-PSI, Switzerland. FAU - Leroy, Ludmila AU - Leroy L AUID- ORCID: 0000-0002-4272-0298 AD - PSI Center for Photon Science, Paul Scherrer Institute, Villigen-PSI, Switzerland. FAU - Moos, Katja Sophia AU - Moos KS AUID- ORCID: 0009-0006-2478-418X AD - PSI Center for Scientific Computing, Theory and Data, Paul Scherrer Institute, Villigen-PSI, Switzerland. AD - Department of Physics, University of Fribourg, Fribourg, Switzerland. FAU - Parusa, Gian AU - Parusa G AUID- ORCID: 0009-0000-1783-195X AD - PSI Center for Scientific Computing, Theory and Data, Paul Scherrer Institute, Villigen-PSI, Switzerland. AD - Department of Physics, University of Fribourg, Fribourg, Switzerland. FAU - Zerdane, Serhane AU - Zerdane S AD - PSI Center for Photon Science, Paul Scherrer Institute, Villigen-PSI, Switzerland. FAU - Mandal, Ritwika AU - Mandal R AD - Institut des Materiaux de Nantes Jean Rouxel (IMN) - UMR 6502, Nantes Universite, CNRS, Nantes, France. FAU - Mariette, Celine AU - Mariette C AUID- ORCID: 0000-0001-8067-9591 AD - ESRF - The European Synchrotron, 71 Avenue des Martyrs, CS40220, Grenoble, Cedex, 38043, France. FAU - Levantino, Matteo AU - Levantino M AUID- ORCID: 0000-0002-1224-4809 AD - ESRF - The European Synchrotron, 71 Avenue des Martyrs, CS40220, Grenoble, Cedex, 38043, France. FAU - Paris, Eugenio AU - Paris E AUID- ORCID: 0000-0002-3418-8828 AD - PSI Center for Photon Science, Paul Scherrer Institute, Villigen-PSI, Switzerland. FAU - Patthey, Luc AU - Patthey L AUID- ORCID: 0000-0001-6101-8069 AD - PSI Center for Photon Science, Paul Scherrer Institute, Villigen-PSI, Switzerland. FAU - Pomjakushina, Ekaterina AU - Pomjakushina E AUID- ORCID: 0000-0002-2446-3830 AD - PSI Center for Neutron and Muon Sciences, Paul Scherrer Institute, Villigen-PSI, Switzerland. FAU - Staub, Urs AU - Staub U AUID- ORCID: 0000-0003-2035-3367 AD - PSI Center for Photon Science, Paul Scherrer Institute, Villigen-PSI, Switzerland. FAU - Hatnean, Monica Ciomaga AU - Hatnean MC AD - PSI Center for Neutron and Muon Sciences, Paul Scherrer Institute, Villigen-PSI, Switzerland. AD - Materials Discovery Laboratory, Department of Materials, ETH Zurich, Zurich, Switzerland. FAU - Schuler, Michael AU - Schuler M AUID- ORCID: 0000-0001-7322-6367 AD - PSI Center for Scientific Computing, Theory and Data, Paul Scherrer Institute, Villigen-PSI, Switzerland. AD - Department of Physics, University of Fribourg, Fribourg, Switzerland. FAU - Razzoli, Elia AU - Razzoli E AUID- ORCID: 0000-0002-8893-972X AD - PSI Center for Photon Science, Paul Scherrer Institute, Villigen-PSI, Switzerland. FAU - Ueda, Hiroki AU - Ueda H AUID- ORCID: 0000-0001-5305-7930 AD - PSI Center for Photon Science, Paul Scherrer Institute, Villigen-PSI, Switzerland. hiroki.ueda@psi.ch. LA - eng PT - Journal Article DEP - 20260929 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 SB - IM COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:18 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/21 00:00 [received] PHST- 2026/09/07 00:00 [accepted] PHST- 2026/10/30 00:18 [entrez] AID - 10.1038/s41467-026-77964-5 [pii] AID - 10.1038/s41467-026-77964-5 [doi] PST - epublish SO - Nat Commun. 2026 Sep 29;17(1):11222. doi: 10.1038/s41467-026-77964-5. PMID- 42957694 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Oct 1 TI - A multiplexed wearable microfluidic platform for non-invasive sweat-inspired monitoring of plant guttation biomarkers. LID - 11267 [pii] LID - 10.1038/s41467-026-77955-6 [doi] AB - Plant guttation fluid provides a chemically informative readout of internal plant status, offering an externally accessible window into plant physiological processes. However, its minute volume, intermittent availability, and susceptibility to evaporation limit its use for continuous biochemical monitoring. Here we introduce PlantIonics, an integrated on-leaf microfluidic electrochemical platform for real-time, non-invasive monitoring of guttation biomarkers and leaf microenvironment. A bioinspired wedge-shaped microchannel with asymmetric wettability achieves rapid (0.6 s) and unidirectional self-driven fluid collection, providing a continuously refreshed sensing interface for dynamic guttation profiling. Combined with a multichannel sensor array and a compact wireless flexible printed circuit board, PlantIonics simultaneously measures guttation-fluid biomarkers (potassium, sodium, glucose) and local leaf microenvironment. Multi-day on-plant studies demonstrate stress-responsive guttation fluctuations that correlate with root-level ionic perturbations and light modulation, demonstrating the platform's capability to monitor dynamic physiological regulation. PlantIonics establishes a sweat-inspired analytical framework for non-invasive plant monitoring, offering opportunities for early stress diagnostics, field phenotyping, and precision agriculture. CI - (c) 2026. The Author(s). FAU - Xue, Bing AU - Xue B AD - Department of Materials Science and Engineering, National University of Singapore, Singapore, Singapore. AD - Institute for Health Innovation and Technology, National University of Singapore, Singapore, Singapore. FAU - Huang, Ziyang AU - Huang Z AD - Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore, Singapore. FAU - Li, Zhiyuan AU - Li Z AUID- ORCID: 0009-0007-5028-8400 AD - Department of Materials Science and Engineering, National University of Singapore, Singapore, Singapore. FAU - Sun, Xue AU - Sun X AD - Institute for Health Innovation and Technology, National University of Singapore, Singapore, Singapore. FAU - Shen, Zhefu AU - Shen Z AUID- ORCID: 0009-0003-6419-9821 AD - Department of Civil and Environmental Engineering, National University of Singapore, Singapore, Singapore. FAU - Luo, Junyi AU - Luo J AD - Department of Materials Science and Engineering, National University of Singapore, Singapore, Singapore. FAU - Ren, Kejing AU - Ren K AD - Department of Materials Science and Engineering, National University of Singapore, Singapore, Singapore. FAU - Zhang, Wei AU - Zhang W AD - Department of Materials Science and Engineering, National University of Singapore, Singapore, Singapore. AD - Institute for Health Innovation and Technology, National University of Singapore, Singapore, Singapore. FAU - Yang, Ruochen AU - Yang R AD - Department of Materials Science and Engineering, National University of Singapore, Singapore, Singapore. AD - Institute for Health Innovation and Technology, National University of Singapore, Singapore, Singapore. FAU - Xiao, Xiao AU - Xiao X AUID- ORCID: 0000-0002-7861-5596 AD - Institute for Health Innovation and Technology, National University of Singapore, Singapore, Singapore. AD - Department of Electrical and Computer Engineering, National University of Singapore, Singapore, Singapore. FAU - Su, Xiaoqian AU - Su X AD - Institute of Flexible Electronics (IFE, Future Technologies), Xiamen University, Xiamen, China. FAU - Liu, Shuoyan AU - Liu S AD - Department of Materials Science and Engineering, National University of Singapore, Singapore, Singapore. FAU - Ge, Anxu AU - Ge A AD - Department of Materials Science and Engineering, National University of Singapore, Singapore, Singapore. FAU - Li, Jinghua AU - Li J AUID- ORCID: 0000-0001-8985-566X AD - Department of Materials Science and Engineering, The Ohio State University, Columbus, OH, USA. FAU - Dharmasena, Ishara AU - Dharmasena I AUID- ORCID: 0000-0002-7959-2840 AD - Wolfson School of Mechanical, Electrical and Manufacturing Engineering, Loughborough University, Loughborough, UK. FAU - Lew, Tedrick Thomas Salim AU - Lew TTS AUID- ORCID: 0000-0002-4815-9921 AD - Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore, Singapore. tedrick@nus.edu.sg. AD - NUS Environmental Research Institute, Singapore, Singapore. tedrick@nus.edu.sg. FAU - Wu, Changsheng AU - Wu C AUID- ORCID: 0000-0002-0921-6356 AD - Department of Materials Science and Engineering, National University of Singapore, Singapore, Singapore. cwu@nus.edu.sg. AD - Institute for Health Innovation and Technology, National University of Singapore, Singapore, Singapore. cwu@nus.edu.sg. AD - Department of Electrical and Computer Engineering, National University of Singapore, Singapore, Singapore. cwu@nus.edu.sg. AD - The N.1 Institute for Health, National University of Singapore, Singapore, Singapore. cwu@nus.edu.sg. LA - eng GR - NRF-NRFF15-2023-0002/National Research Foundation Singapore (National Research Foundation-Prime Minister's office, Republic of Singapore)/ PT - Journal Article DEP - 20261001 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 RN - 0 (Biomarkers) RN - RWP5GA015D (Potassium) RN - 9NEZ333N27 (Sodium) RN - IY9XDZ35W2 (Glucose) SB - IM MH - *Biomarkers/analysis MH - *Plant Leaves/metabolism/physiology/chemistry MH - *Sweat/chemistry MH - *Wearable Electronic Devices MH - *Microfluidics/instrumentation/methods MH - Potassium/analysis MH - Sodium/analysis MH - Glucose/analysis MH - Wettability MH - *Microfluidic Analytical Techniques/instrumentation COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:18 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/10 00:00 [received] PHST- 2026/09/07 00:00 [accepted] PHST- 2026/10/30 00:18 [entrez] AID - 10.1038/s41467-026-77955-6 [pii] AID - 10.1038/s41467-026-77955-6 [doi] PST - epublish SO - Nat Commun. 2026 Oct 1;17(1):11267. doi: 10.1038/s41467-026-77955-6. PMID- 42957684 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 29 TI - A role for selective ER autophagy in gametogenic rejuvenation revealed by high-throughput lifespan profiling. LID - 11215 [pii] LID - 10.1038/s41467-026-77993-0 [doi] AB - During mitotic growth, Saccharomyces cerevisiae cells age by dividing asymmetrically, producing young daughter cells while retaining age-associated damage in the mother cell, which will eventually become senescent. Gametogenesis naturally and fully resets precursor cell lifespan, even for replicatively aged cells. However, the mechanisms responsible for gametogenic rejuvenation have remained mysterious. This is partly due to the existing methods to quantify lifespan resetting in this context, which are limited to low-throughput and labor-intensive approaches. Here, we introduce a high-throughput assay that allows systematic characterization of factors required for gametogenic rejuvenation. With this approach, we show that we can sensitively measure a wide range of gamete replicative lifespans that are consistent with established mutants. Excitingly, we also discover that Atg39 and Atg40, receptors involved in selective autophagy of the ER, are molecular determinants of gametogenic rejuvenation. This technique will enable systematic identification of the molecular factors that drive gametogenic rejuvenation. CI - (c) 2026. The Author(s). FAU - Spiri, Silvan AU - Spiri S AUID- ORCID: 0000-0002-7124-3056 AD - Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA. silvan.spiri@berkeley.edu. FAU - Sing, Tina Lynn AU - Sing TL AD - Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA. FAU - Phung, Nhi AU - Phung N AUID- ORCID: 0009-0000-5639-852X AD - Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA. FAU - Goodman, Jay S AU - Goodman JS AUID- ORCID: 0000-0002-4788-3918 AD - Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA. FAU - Unal, Elcin AU - Unal E AUID- ORCID: 0000-0002-6768-609X AD - Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA. elcin@berkeley.edu. AD - California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, Berkeley, CA, USA. elcin@berkeley.edu. AD - Center for Computational Biology, University of California, Berkeley, Berkeley, CA, USA. elcin@berkeley.edu. FAU - Brar, Gloria Ann AU - Brar GA AUID- ORCID: 0000-0002-8560-9581 AD - Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA. gabrar@berkeley.edu. AD - California Institute for Quantitative Biosciences (QB3), University of California, Berkeley, Berkeley, CA, USA. gabrar@berkeley.edu. AD - Center for Computational Biology, University of California, Berkeley, Berkeley, CA, USA. gabrar@berkeley.edu. LA - eng GR - R01AG071869/Foundation for the National Institutes of Health (Foundation for the National Institutes of Health, Inc.)/ GR - R01AG071801/Foundation for the National Institutes of Health (Foundation for the National Institutes of Health, Inc.)/ GR - P500PB_214421/Schweizerischer Nationalfonds zur Forderung der Wissenschaftlichen Forschung (Swiss National Science Foundation)/ PT - Journal Article DEP - 20260929 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 RN - 0 (Saccharomyces cerevisiae Proteins) RN - 0 (Autophagy-Related Proteins) SB - IM MH - *Autophagy/physiology/genetics MH - *Saccharomyces cerevisiae/genetics/metabolism/physiology/cytology MH - Saccharomyces cerevisiae Proteins/metabolism/genetics MH - *Endoplasmic Reticulum/metabolism MH - *Gametogenesis/physiology/genetics MH - Autophagy-Related Proteins/metabolism/genetics MH - Mutation MH - High-Throughput Screening Assays COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:17 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/11 00:00 [received] PHST- 2026/09/07 00:00 [accepted] PHST- 2026/10/30 00:17 [entrez] AID - 10.1038/s41467-026-77993-0 [pii] AID - 10.1038/s41467-026-77993-0 [doi] PST - epublish SO - Nat Commun. 2026 Sep 29;17(1):11215. doi: 10.1038/s41467-026-77993-0. PMID- 42957678 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 28 TI - Adaptive personalization for wearable non-invasive continuous glucose sensors. LID - 11211 [pii] LID - 10.1038/s41467-026-74531-w [doi] AB - Accurate continuous glucose monitoring (CGM) is essential for diabetes management, yet non-invasive CGM remains challenging. Universal "one-sensor-fits-all" approaches often fail, because correlations between non-invasive physiological readouts and blood glucose (BG) are highly individualized. Here, we propose a personalized "Adaptive Non-invasive CGM" methodology, which integrates a short-term minimally invasive CGM (MI-CGM) module for measuring interstitial fluid glucose, and a long-term non-invasive CGM (NI-CGM) module based on metabolic heat conformation. During a ~2-days training, the NI-CGM learns accurate sensing from the MI-CGM via GRU-based AI model, forming patient-specific sensor. The MI-CGM is then removed, leaving only the NI-CGM for long-term monitoring. In a 5-day CGM test, the system achieved MARD ~15.6%. This method also demonstrated good reproducibility and maintained reasonable accuracy (MARD ~23.9%) during long-term interval use (>2 months). This personalized approach offers a pathway from conventional "one-sensor-fits-all" strategies toward individualized sensor development, potentially mitigating inter-patient variability and non-invasive monitoring inaccuracies. CI - (c) 2026. The Author(s). FAU - Yao, Chuanjie AU - Yao C AD - State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, China. FAU - Gao, Lukang AU - Gao L AD - State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, China. FAU - Wang, Xinze AU - Wang X AD - State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, China. FAU - Wang, Haolin AU - Wang H AD - State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, China. FAU - Huang, Shuang AU - Huang S AD - State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, China. AD - Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument, School of Biomedical Engineering, Sun Yat-sen University, Shenzhen, China. FAU - Li, Guanbin AU - Li G AD - Computer Science and Engineering, Sun Yat-sen University, Guangzhou, China. FAU - Han, Xiongqi AU - Han X AD - Shenzhen SiSensing Co., Ltd, Shenzhen, China. FAU - Yang, Hanqi AU - Yang H AD - State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, China. FAU - Liu, Zhengjie AU - Liu Z AD - State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, China. FAU - Liang, Shanshan AU - Liang S AD - National Innovation Center for Advanced Medical Devices, Shenzhen, China. FAU - Yun, Peng AU - Yun P AD - Department of Endocrinology, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China. FAU - Wang, Yunuo AU - Wang Y AD - The First Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University, Guangzhou, China. FAU - Cai, Yeyun AU - Cai Y AD - National Key Lab of Autonomous Intelligent Unmanned Systems, Beijing Institute of Technology, Beijing, China. FAU - Zhang, Peikai AU - Zhang P AD - Shenzhen SiSensing Co., Ltd, Shenzhen, China. FAU - Wu, Yuanqing AU - Wu Y AD - School of Intelligent Systems Engineering, Sun Yat-Sen University, Shenzhen, China. FAU - Li, Mingqiang AU - Li M AUID- ORCID: 0000-0002-5178-4138 AD - Laboratory of Biomaterials and Translational Medicine, Center for Nanomedicine, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. FAU - Wang, Ji AU - Wang J AUID- ORCID: 0000-0002-8631-3264 AD - The First Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University, Guangzhou, China. FAU - Chen, Hui-Jiuan AU - Chen HJ AD - State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, China. FAU - Huang, Xinshuo AU - Huang X AD - State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, China. huangxsh35@mail.sysu.edu.cn. FAU - Xie, Xi AU - Xie X AUID- ORCID: 0000-0001-7406-8444 AD - State Key Laboratory of Optoelectronic Materials and Technologies, Guangdong Province Key Laboratory of Display Material and Technology, School of Electronics and Information Technology, Sun Yat-sen University, Guangzhou, China. xiexi27@mail.sysu.edu.cn. AD - Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument, School of Biomedical Engineering, Sun Yat-sen University, Shenzhen, China. xiexi27@mail.sysu.edu.cn. AD - The First Affiliated Hospital of Sun Yat-sen University, Sun Yat-sen University, Guangzhou, China. xiexi27@mail.sysu.edu.cn. LA - eng GR - T2225010/National Natural Science Foundation of China (National Science Foundation of China)/ GR - T2541040/National Natural Science Foundation of China (National Science Foundation of China)/ GR - T2541039/National Natural Science Foundation of China (National Science Foundation of China)/ GR - T2541006/National Natural Science Foundation of China (National Science Foundation of China)/ PT - Journal Article DEP - 20260928 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 RN - 0 (Blood Glucose) SB - IM MH - *Continuous Glucose Monitoring/instrumentation/methods MH - Humans MH - *Blood Glucose/analysis MH - *Wearable Electronic Devices MH - *Blood Glucose Self-Monitoring/instrumentation/methods MH - Reproducibility of Results MH - *Biosensing Techniques/methods/instrumentation COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:17 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/08/28 00:00 [received] PHST- 2026/06/01 00:00 [accepted] PHST- 2026/10/30 00:17 [entrez] AID - 10.1038/s41467-026-74531-w [pii] AID - 10.1038/s41467-026-74531-w [doi] PST - epublish SO - Nat Commun. 2026 Sep 28;17(1):11211. doi: 10.1038/s41467-026-74531-w. PMID- 42957675 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 29 TI - Deep-learning single-cell profiling uncovers interbacterial and drug-driven reshaping of pathogen virulence. LID - 11236 [pii] LID - 10.1038/s41467-026-77989-w [doi] AB - Bacterial invasion and genotoxicity are key contributors to infectious disease progression and carcinogenesis. Yet current methods do not quantify these processes systematically and at single-cell resolution, limiting our ability to explore how interplay of virulence factors and drug effects shape pathogenicity. Here, we present MALVINA, a high-throughput deep-learning workflow integrating host-pathogen co-culture and high-content imaging to jointly measure bacterial invasion and host DNA damage. Applied to Escherichia coli isolates and human colorectal epithelial cells, we demonstrate MALVINA's versatility by deciphering (1) strains with elevated invasive or genotoxic potential, (2) interbacterial modulation of virulence, (3) pro-virulent drug effects, (4) virulence-targeting drugs, and (5) bacterial potentiation of drug toxicity. MALVINA revealed strain-specific virulence profiles and colibactin-dependent suppression of competitors' invasion coupled with enhanced genotoxicity. It also enabled identification of drug-induced modulation of bacterial invasion and genotoxicity, offering a new perspective for virulence-aware drug profiling. Overall, MALVINA offers an adaptable framework for dissecting pathogen-host-drug interactions. CI - (c) 2026. The Author(s). FAU - Bognar, Bence AU - Bognar B AUID- ORCID: 0009-0004-9091-3467 AD - Synthetic and Systems Biology Unit, Institute of Biochemistry, HUN-REN Biological Research Centre, Szeged, Hungary. AD - HCEMM-BRC Pharmacodynamic Drug Interaction Research Group, Szeged, Hungary. AD - Doctoral School of Biology, University of Szeged, Szeged, Hungary. FAU - Kovacs, Terezia AU - Kovacs T AUID- ORCID: 0000-0002-1078-3315 AD - Synthetic and Systems Biology Unit, Institute of Biochemistry, HUN-REN Biological Research Centre, Szeged, Hungary. AD - HCEMM-BRC Pharmacodynamic Drug Interaction Research Group, Szeged, Hungary. FAU - Muresan, Reka AU - Muresan R AD - HCEMM Cancer Microbiome Core Group, Szeged, Hungary. FAU - Migh, Ede AU - Migh E AD - Synthetic and Systems Biology Unit, Institute of Biochemistry, HUN-REN Biological Research Centre, Szeged, Hungary. FAU - Balogh, Timea AU - Balogh T AD - Synthetic and Systems Biology Unit, Institute of Biochemistry, HUN-REN Biological Research Centre, Szeged, Hungary. FAU - Kovacs, Nandor Milan AU - Kovacs NM AD - Synthetic and Systems Biology Unit, Institute of Biochemistry, HUN-REN Biological Research Centre, Szeged, Hungary. AD - HCEMM-BRC Pharmacodynamic Drug Interaction Research Group, Szeged, Hungary. FAU - Somogyi, Aron AU - Somogyi A AD - HCEMM Cancer Microbiome Core Group, Szeged, Hungary. FAU - Molnar, Arpad AU - Molnar A AD - Synthetic and Systems Biology Unit, Institute of Biochemistry, HUN-REN Biological Research Centre, Szeged, Hungary. AD - HCEMM-BRC Pharmacodynamic Drug Interaction Research Group, Szeged, Hungary. FAU - Csorgo, Balint AU - Csorgo B AUID- ORCID: 0000-0003-0397-6845 AD - Synthetic and Systems Biology Unit, Institute of Biochemistry, HUN-REN Biological Research Centre, Szeged, Hungary. FAU - Pal, Csaba AU - Pal C AUID- ORCID: 0000-0002-5187-9903 AD - Synthetic and Systems Biology Unit, Institute of Biochemistry, HUN-REN Biological Research Centre, Szeged, Hungary. FAU - Horvath, Peter AU - Horvath P AD - Synthetic and Systems Biology Unit, Institute of Biochemistry, HUN-REN Biological Research Centre, Szeged, Hungary. AD - Single-Cell Technologies Ltd., Szeged, Hungary. AD - Institute of AI for Health, Helmholtz Zentrum Munchen, Neuherberg, Germany. AD - Institute for Molecular Medicine Finland, University of Helsinki, Helsinki, Finland. FAU - Juhasz, Szilvia AU - Juhasz S AD - Synthetic and Systems Biology Unit, Institute of Biochemistry, HUN-REN Biological Research Centre, Szeged, Hungary. szilvia.juhasz@hcemm.eu. AD - HCEMM Cancer Microbiome Core Group, Szeged, Hungary. szilvia.juhasz@hcemm.eu. FAU - Lazar, Viktoria AU - Lazar V AUID- ORCID: 0000-0002-9778-941X AD - Synthetic and Systems Biology Unit, Institute of Biochemistry, HUN-REN Biological Research Centre, Szeged, Hungary. lazar.viktoria@brc.hu. AD - HCEMM-BRC Pharmacodynamic Drug Interaction Research Group, Szeged, Hungary. lazar.viktoria@brc.hu. LA - eng PT - Journal Article DEP - 20260929 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 RN - 0 (Virulence Factors) RN - 0 (Anti-Bacterial Agents) RN - 0 (colibactin) RN - 0 (Peptides) RN - 0 (Polyketides) SB - IM MH - Humans MH - Virulence/drug effects/genetics MH - *Single-Cell Analysis/methods MH - *Host-Pathogen Interactions/drug effects MH - *Escherichia coli/pathogenicity/drug effects/genetics MH - DNA Damage/drug effects MH - Virulence Factors/metabolism/genetics MH - *Deep Learning MH - *Anti-Bacterial Agents/pharmacology MH - Epithelial Cells/microbiology MH - Escherichia coli Infections/microbiology/drug therapy MH - Peptides MH - Polyketides COIS- Competing interests: P.H. is the founder and a shareholder of Single-Cell Technologies Ltd., a biodata analysis company that owns and develops the BIAS software. HUN-REN Biological Research Centre, HCEMM and Single-Cell Technologies Ltd. have filed a patent application (PCT/HU2026/050077, pending) relating to the infection-state-stratified single-cell classification and integrated pathogen invasion-host response profiling methods described in this manuscript. V.L., S.J., B.B., T.K. and P.H. are named inventors on this application. The remaining authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:16 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/11/25 00:00 [received] PHST- 2026/09/04 00:00 [accepted] PHST- 2026/10/30 00:16 [entrez] AID - 10.1038/s41467-026-77989-w [pii] AID - 10.1038/s41467-026-77989-w [doi] PST - epublish SO - Nat Commun. 2026 Sep 29;17(1):11236. doi: 10.1038/s41467-026-77989-w. PMID- 42957673 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2041-4889 (Electronic) VI - 17 IP - 1 DP - 2026 Jul 31 TI - Targeting RBM41-mediated CKMT1B mRNA stability triggers disulfidptosis and suppresses tongue squamous cell carcinoma. LID - 914 [pii] LID - 10.1038/s41419-026-09166-2 [doi] AB - Clarifying the mechanisms by which metabolic stress induces cell death in tumor cells is essential for the development of effective therapies targeting tongue squamous cell carcinoma (TSCC). In this study, we demonstrate that the RNA-binding motif protein 41 (RBM41) stabilizes CKMT1B mRNA through its RRM1 domain, thereby conferring resistance to disulfidptosis under conditions of glucose deprivation in TSCC. We explored the expression and functional role of RBM41 using both in vitro and in vivo models, which included RBM41-knockout, RRM1-domain-knockout, and RBM41-overexpressing TSCC cell lines. Our findings reveal that RBM41-mediated stabilization of CKMT1B mRNA is crucial for protecting TSCC cells from disulfidptosis during metabolic stress. Based on this mechanism, we designed a structure-specific inhibitory small molecule, L759-0762, which disrupts the interaction between RBM41 and CKMT1B mRNA. L759-0762 selectively sensitizes TSCC cells to disulfidptosis under glucose deprivation by abolishing the protective function of RBM41. Collectively, our findings identify RBM41 as a viable therapeutic target and demonstrate that pharmacological disruption of its interaction with CKMT1B mRNA represents a promising strategy for inducing disulfidptosis in TSCC. CI - (c) 2026. The Author(s). FAU - Tao, Boqiang AU - Tao B AD - Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jilin University, Changchun, China. AD - Jilin Provincial Key Laboratory of Oral Biomedical Engineering, Hospital of Stomatology, Jilin University, Changchun, China. FAU - Hao, Ming AU - Hao M AD - Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jilin University, Changchun, China. AD - Jilin Provincial Key Laboratory of Oral Biomedical Engineering, Hospital of Stomatology, Jilin University, Changchun, China. FAU - Hao, Yang AU - Hao Y AD - Laboratory Animal Center, College of Animal Science, Jilin University, Changchun, China. FAU - Zhang, Shuhan AU - Zhang S AD - Laboratory Animal Center, College of Animal Science, Jilin University, Changchun, China. FAU - Xiao, Zhaorong AU - Xiao Z AD - Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jilin University, Changchun, China. AD - Jilin Provincial Key Laboratory of Oral Biomedical Engineering, Hospital of Stomatology, Jilin University, Changchun, China. FAU - Shi, He AU - Shi H AD - Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jilin University, Changchun, China. AD - Jilin Provincial Key Laboratory of Oral Biomedical Engineering, Hospital of Stomatology, Jilin University, Changchun, China. FAU - Li, Qirong AU - Li Q AD - Laboratory Animal Center, College of Animal Science, Jilin University, Changchun, China. FAU - Qin, Yiwen AU - Qin Y AD - Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jilin University, Changchun, China. AD - Jilin Provincial Key Laboratory of Oral Biomedical Engineering, Hospital of Stomatology, Jilin University, Changchun, China. FAU - Wang, Dongxu AU - Wang D AUID- ORCID: 0000-0003-0464-1596 AD - Laboratory Animal Center, College of Animal Science, Jilin University, Changchun, China. wang_dong_xu@jlu.edu.cn. FAU - Liu, Weiwei AU - Liu W AUID- ORCID: 0000-0001-5082-8963 AD - Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jilin University, Changchun, China. liuweiw@jlu.edu.cn. AD - Jilin Provincial Key Laboratory of Oral Biomedical Engineering, Hospital of Stomatology, Jilin University, Changchun, China. liuweiw@jlu.edu.cn. LA - eng GR - 20240305037YY/Department of Science and Technology of Jilin Province (Jilin Province Science and Technology Department)/ PT - Journal Article DEP - 20260731 PL - England TA - Cell Death Dis JT - Cell death & disease JID - 101524092 RN - 0 (RNA-Binding Proteins) RN - 0 (RNA, Messenger) RN - IY9XDZ35W2 (Glucose) SB - IM MH - Humans MH - *Tongue Neoplasms/genetics/pathology/metabolism MH - *RNA-Binding Proteins/metabolism/genetics MH - *Disulfidptosis/drug effects/genetics MH - Cell Line, Tumor MH - *RNA Stability/genetics/drug effects MH - *Carcinoma, Squamous Cell/genetics/pathology/metabolism MH - RNA, Messenger/metabolism/genetics MH - Animals MH - Glucose/deficiency MH - Gene Expression Regulation, Neoplastic MH - Mice COIS- Competing interests: The authors declare no competing interests. Ethical approval and consent to participate: All animal experiments were approved by the Animal Ethics and Welfare Committee of Jilin University (No. SY20250729) and conducted under supervision, adhering to the ethical welfare requirements for experimental animals set forth by Jilin University and national regulations. The breeding was strictly conducted in accordance with GB14925 standards. The tongue squamous cell carcinoma specimens were obtained from the College of Stomatology at Jilin University, following the protocol approved by the hospital's ethics committee (Clinical Ethics Approval No. SJDKQ2024026). All patients provided informed consent, and their information has been kept strictly confidential. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:16 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/12/04 00:00 [received] PHST- 2026/07/24 00:00 [accepted] PHST- 2026/07/15 00:00 [revised] PHST- 2026/10/30 00:16 [entrez] AID - 10.1038/s41419-026-09166-2 [pii] AID - 10.1038/s41419-026-09166-2 [doi] PST - epublish SO - Cell Death Dis. 2026 Jul 31;17(1):914. doi: 10.1038/s41419-026-09166-2. PMID- 42957670 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261030 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 30 TI - Chiral trions in enantiopure single-wall carbon nanotubes. LID - 11235 [pii] LID - 10.1038/s41467-026-78073-z [doi] AB - A trion is a charged exciton, which can be observed in many low-dimensional semiconductors. Circular dichroism (CD) of trions was demonstrated based on optical transitions following their spin selection rules, such as spin states of semiconductor quantum dots and valley spin polarization in monolayer two-dimensional materials. However, the configurational CD of trions, which is uniquely caused by chiral structures, has never been observed. In this study, the CD of trions in enantiopure single-wall carbon nanotubes (SWCNTs) is demonstrated. Depending on the right- or left-handedness of the SWCNTs, the sign of the trion CD changes and is the same as that of the original exciton CD. Consequently, chiral trions exist in chiral SWCNTs. CI - (c) 2026. The Author(s). FAU - Fujinami, Hiroyuki AU - Fujinami H AD - Department of Physics, Tokyo Metropolitan University, Tokyo, Japan. FAU - Kusaba, Satoshi AU - Kusaba S AUID- ORCID: 0000-0002-5313-4817 AD - Department of Physics, Tokyo Metropolitan University, Tokyo, Japan. FAU - Konabe, Satoru AU - Konabe S AD - Department of Chemical Science and Technology, Hosei University, Tokyo, Japan. FAU - Gao, Weilu AU - Gao W AUID- ORCID: 0000-0003-3139-034X AD - Department of Electrical and Computer Engineering, The University of Utah, Salt Lake City, UT, USA. FAU - Zaumseil, Jana AU - Zaumseil J AUID- ORCID: 0000-0002-2048-217X AD - Institute for Physical Chemistry, Heidelberg University, Heidelberg, Germany. FAU - Yanagi, Kazuhiro AU - Yanagi K AUID- ORCID: 0000-0002-7609-1493 AD - Department of Physics, Tokyo Metropolitan University, Tokyo, Japan. yanagi-kazuhiro@tmu.ac.jp. LA - eng GR - JP21H05017/MEXT | Japan Society for the Promotion of Science (JSPS)/ GR - JP23H00259/MEXT | Japan Society for the Promotion of Science (JSPS)/ GR - JP24H01200/MEXT | Japan Society for the Promotion of Science (JSPS)/ GR - JPJSBP120252302/MEXT | Japan Society for the Promotion of Science (JSPS)/ GR - JPJSJRP20221202/MEXT | Japan Society for the Promotion of Science (JSPS)/ GR - JPMJCR2544/MEXT | JST | Core Research for Evolutional Science and Technology (CREST)/ GR - JPMJAP2310/MEXT | Japan Science and Technology Agency (JST)/ GR - MIYAKO-MIRAI Project/Tokyo Metropolitan University (Tokyo Toritsu Daigaku)/ GR - 2230727/National Science Foundation (NSF)/ GR - SFB 1225/3/Deutsche Forschungsgemeinschaft (German Research Foundation)/ PT - Journal Article DEP - 20260930 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 SB - IM COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:16 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/08 00:00 [received] PHST- 2026/09/14 00:00 [accepted] PHST- 2026/10/30 00:16 [entrez] AID - 10.1038/s41467-026-78073-z [pii] AID - 10.1038/s41467-026-78073-z [doi] PST - epublish SO - Nat Commun. 2026 Sep 30;17(1):11235. doi: 10.1038/s41467-026-78073-z. PMID- 42957666 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261030 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Oct 28 TI - Ketene as a gas phase intermediate in acetic acid ketonization on TiO(2). LID - 11209 [pii] LID - 10.1038/s41467-026-77945-8 [doi] AB - Reactive gas-phase intermediates are often underappreciated in heterogeneous catalysis, despite their potential to control key catalytic steps. Here, we provide direct experimental evidence that ketene can act as an intermediate in acetic acid ketonization, a mechanism that has long been debated. Using faceted TiO(2)(101) nanoparticles assembled into layers of controlled thickness, we show that ketene, formed from surface acetate, migrates through interparticle voids via desorption-readsorption events before reacting with surface acetate to yield acetone. Surface science and reactor studies reveal a direct correlation between ketene and acetone production and the depth to which the particle layers are saturated. Ab initio calculations support ketene's reactivity via low-barrier coupling pathways that converge on a common beta-ketoacid intermediate. Here, we show that ketene can act as a mobile gas-phase intermediate and underscore the broader importance of spatially decoupled elementary steps in determining reactivity and selectivity in catalytic systems. CI - (c) 2026. Battelle Memorial Institute and Jiayan Xu, Annabella Selloni. FAU - Xu, Jiayan AU - Xu J AUID- ORCID: 0000-0001-9897-5778 AD - Department of Chemistry, Princeton University, Princeton, NJ, USA. FAU - DeBenedetti, William Ji AU - DeBenedetti WJ AD - Physical and Computational Sciences Directorate and Institute for Integrated Catalysis, Pacific Northwest National Laboratory, Richland, WA, USA. AD - Plutonium Supply & Disposition (PSD-4), Los Alamos National Laboratory, Los Alamos, New Mexico, USA. FAU - Wang, Xingyu AU - Wang X AUID- ORCID: 0000-0003-4195-0056 AD - Physical and Computational Sciences Directorate and Institute for Integrated Catalysis, Pacific Northwest National Laboratory, Richland, WA, USA. AD - Commonwealth Fusion Systems, Devens, MA, USA. FAU - Wu, Yiqing AU - Wu Y AUID- ORCID: 0000-0001-5014-3761 AD - Physical and Computational Sciences Directorate and Institute for Integrated Catalysis, Pacific Northwest National Laboratory, Richland, WA, USA. FAU - Kovarik, Libor AU - Kovarik L AD - Physical and Computational Sciences Directorate and Institute for Integrated Catalysis, Pacific Northwest National Laboratory, Richland, WA, USA. FAU - O'Connor, Christopher R AU - O'Connor CR AUID- ORCID: 0000-0002-9224-9342 AD - Physical and Computational Sciences Directorate and Institute for Integrated Catalysis, Pacific Northwest National Laboratory, Richland, WA, USA. FAU - Gao, Feng AU - Gao F AUID- ORCID: 0000-0002-8450-3419 AD - Physical and Computational Sciences Directorate and Institute for Integrated Catalysis, Pacific Northwest National Laboratory, Richland, WA, USA. FAU - Szanyi, Janos AU - Szanyi J AUID- ORCID: 0000-0002-8442-5465 AD - Physical and Computational Sciences Directorate and Institute for Integrated Catalysis, Pacific Northwest National Laboratory, Richland, WA, USA. FAU - Wang, Yong AU - Wang Y AUID- ORCID: 0000-0002-8460-7410 AD - Physical and Computational Sciences Directorate and Institute for Integrated Catalysis, Pacific Northwest National Laboratory, Richland, WA, USA. AD - Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington, USA. FAU - Selloni, Annabella AU - Selloni A AUID- ORCID: 0000-0001-5896-3158 AD - Department of Chemistry, Princeton University, Princeton, NJ, USA. aselloni@princeton.edu. FAU - Kimmel, Greg A AU - Kimmel GA AUID- ORCID: 0000-0003-4447-2440 AD - Physical and Computational Sciences Directorate and Institute for Integrated Catalysis, Pacific Northwest National Laboratory, Richland, WA, USA. gregory.kimmel@pnnl.gov. FAU - Dohnalek, Zdenek AU - Dohnalek Z AUID- ORCID: 0000-0002-5999-7867 AD - Physical and Computational Sciences Directorate and Institute for Integrated Catalysis, Pacific Northwest National Laboratory, Richland, WA, USA. zdenek.dohnalek@pnnl.gov. AD - Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, Washington, USA. zdenek.dohnalek@pnnl.gov. LA - eng GR - DESC0007347/DOE | SC | Basic Energy Sciences (BES)/ GR - FWP 47319/DOE | SC | Basic Energy Sciences (BES)/ PT - Journal Article DEP - 20261028 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 SB - IM COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:16 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/01/25 00:00 [received] PHST- 2026/09/01 00:00 [accepted] PHST- 2026/10/30 00:16 [entrez] AID - 10.1038/s41467-026-77945-8 [pii] AID - 10.1038/s41467-026-77945-8 [doi] PST - epublish SO - Nat Commun. 2026 Oct 28;17(1):11209. doi: 10.1038/s41467-026-77945-8. PMID- 42957662 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2049-3169 (Electronic) IS - 1674-2818 (Linking) VI - 18 IP - 1 DP - 2026 Oct 29 TI - Bone anabolic agents repair alveolar bone damaged by diabetes in mice. LID - 68 [pii] LID - 10.1038/s41368-026-00470-3 [doi] AB - Type 2 diabetes (T2D) is a chronic metabolic disorder that significantly impacts skeletal health, including bones of the oral cavity, where alveolar bone is particularly susceptible due to its high remodeling rate and sensitivity to systemic metabolic changes. Using a mouse model of established T2D induced by high-fat diet and streptozotocin, we investigated the effects of the FDA-approved bone anabolic agents parathyroid hormone (PTH) and abaloparatide (ABL) on alveolar bone. In non-diabetic mice, PTH and ABL stimulated both bone formation and resorption, resulting in no net change in alveolar bone mass. In contrast, these agents selectively increased bone formation in T2D mice without further enhancing the already elevated resorption, leading to net alveolar bone gain. This anabolic effect involves suppression of sclerostin and Dickkopf-1 (Dkk-1) in osteocytes and activation of Wnt signaling in periodontal ligament cells. Studies using DMP1-Cre;PTH1R(fl/fl) mice demonstrated that osteocytic PTH1R is essential for the anabolic effects but not for the bone resorption-promoting effects of PTH and ABL. These findings reveal a dual mechanism of PTH1R signaling in oral bone remodeling and suggest therapeutic strategies to restore alveolar bone and improve tooth retention and implant osseointegration in diabetic patients. CI - (c) 2026. The Author(s). FAU - Turkkahraman, Hakan AU - Turkkahraman H AD - Department of Orthodontics and Oral Facial Genetics, Indiana University School of Dentistry, Indianapolis, IN, USA. FAU - Walton, Carson Joseph AU - Walton CJ AD - Department of Otolaryngology-Head & Neck Surgery, Indiana University School of Medicine, Indianapolis, IN, USA. FAU - Koizumi, Yukiko AU - Koizumi Y AD - Department of Otolaryngology-Head & Neck Surgery, Indiana University School of Medicine, Indianapolis, IN, USA. AD - Department of Anatomy, Cell Biology & Physiology, Indiana University School of Medicine, Indianapolis, IN, USA. AD - Indiana University School of Medicine, Indiana Center for Musculoskeletal Health, Indianapolis, IN, USA. FAU - Zhu, Tianli AU - Zhu T AD - Department of Biomedical Sciences and Comprehensive Care, Indiana University School of Dentistry, Indianapolis, IN, USA. FAU - Akel, Nisreen AU - Akel N AD - University of Arkansas for Medical Sciences, Department of Physiology and Cell Biology, Little Rock, AR, USA. FAU - Yuan, Xue AU - Yuan X AUID- ORCID: 0000-0002-8063-9431 AD - Department of Otolaryngology-Head & Neck Surgery, Indiana University School of Medicine, Indianapolis, IN, USA. yuanxue@iu.edu. AD - Indiana University School of Medicine, Indiana Center for Musculoskeletal Health, Indianapolis, IN, USA. yuanxue@iu.edu. FAU - Bellido, Teresita M AU - Bellido TM AD - University of Arkansas for Medical Sciences, Department of Physiology and Cell Biology, Little Rock, AR, USA. TMBellido@uams.edu. AD - Central Arkansas Veterans Healthcare System, John L. McClellan, Little Rock, AR, USA. TMBellido@uams.edu. LA - eng GR - R03DE033059/U.S. Department of Health & Human Services | NIH | National Institute of Dental and Craniofacial Research (NIDCR)/ GR - T32 AR065971/U.S. Department of Health & Human Services | NIH | National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)/ GR - R01-AR059357/U.S. Department of Health & Human Services | NIH | National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)/ PT - Journal Article DEP - 20261029 PL - India TA - Int J Oral Sci JT - International journal of oral science JID - 101504351 RN - 0 (Parathyroid Hormone) RN - 0 (Parathyroid Hormone-Related Protein) RN - 0 (Anabolic Agents) RN - 0 (Sost protein, mouse) RN - 0 (Intercellular Signaling Peptides and Proteins) RN - 0 (Receptor, Parathyroid Hormone, Type 1) RN - 0 (Adaptor Proteins, Signal Transducing) SB - IM MH - Animals MH - Mice MH - *Parathyroid Hormone/pharmacology MH - *Parathyroid Hormone-Related Protein/pharmacology MH - *Anabolic Agents/pharmacology MH - *Diabetes Mellitus, Experimental/complications MH - *Alveolar Bone Loss/drug therapy/etiology MH - *Diabetes Mellitus, Type 2/complications MH - Osteogenesis/drug effects MH - *Alveolar Process/drug effects MH - Intercellular Signaling Peptides and Proteins/metabolism MH - Bone Remodeling/drug effects MH - Male MH - Receptor, Parathyroid Hormone, Type 1/metabolism MH - Wnt Signaling Pathway/drug effects MH - Adaptor Proteins, Signal Transducing COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:16 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/03/25 00:00 [received] PHST- 2026/09/07 00:00 [accepted] PHST- 2026/09/04 00:00 [revised] PHST- 2026/10/30 00:16 [entrez] AID - 10.1038/s41368-026-00470-3 [pii] AID - 10.1038/s41368-026-00470-3 [doi] PST - epublish SO - Int J Oral Sci. 2026 Oct 29;18(1):68. doi: 10.1038/s41368-026-00470-3. PMID- 42957652 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2059-3635 (Electronic) IS - 2059-3635 (Linking) VI - 11 IP - 1 DP - 2026 Oct 30 TI - Cancer-associated fibroblasts: origins, functional heterogeneity and therapeutic potential. LID - 476 [pii] LID - 10.1038/s41392-026-02743-z [doi] AB - Cancer-associated fibroblasts (CAFs) are integral components of the tumor microenvironment (TME) and are increasingly recognized as critical regulators of nearly every hallmark of cancer. Once viewed as a uniform, protumorigenic cell type, it is now clear that CAFs comprise a spectrum of dynamic and plastic functional states with both tumor-promoting and tumor-restraining capacities. This functional duality, conceptualized within the framework of cancer as a "wound that fails to heal", is dictated by their diverse cellular origins, the specific activation pathways engaged (such as TGF-beta and PDGF), and the evolving cues from the TME. In this Review, we dissect the multifaceted roles of CAFs, from their promotion of cancer cell proliferation, invasion and angiogenesis through secreted factors and extensive extracellular matrix (ECM) remodeling, to their profound influence on the immune landscape. We detail how distinct CAF subsets, such as myofibroblastic (myCAFs) and inflammatory (iCAFs) CAFs, orchestrate an immunosuppressive niche and contribute to therapeutic resistance. We also explore the compelling evidence for tumor-suppressive CAF functions, which have catalyzed a paradigm shift in therapeutic strategies-moving away from indiscriminate CAF ablation towards nuanced approaches aimed at reprogramming their state. These strategies include modulating key signaling pathways, normalizing the CAF-remodeled ECM, and exploiting unique metabolic vulnerabilities. Finally, we discuss how advances in single-cell and spatial transcriptomics are resolving this complexity, paving the way for the development of precision therapies that can selectively neutralize pro-tumorigenic CAF activities while harnessing their beneficial functions to improve patient outcomes. CI - (c) 2026. The Author(s). FAU - Zhou, Liting AU - Zhou L AD - State Key Laboratory of Metabolic Dysregulation & the Prevention and Treatment of Esophageal Cancer; The School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China. AD - Department of Medical Genetics & Cell Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China. AD - Tianjian Laboratory of Advanced Biomedical Sciences, Institute of Advanced Bio-medical Sciences, Cancer Prevention and Treatment Lab, Zhengzhou University, Zhengzhou, China. AD - China‒US (Henan) Hormel Cancer Institute, Zhengzhou, Henan, China. FAU - Tang, Mengyue AU - Tang M AD - State Key Laboratory of Metabolic Dysregulation & the Prevention and Treatment of Esophageal Cancer; The School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China. AD - Tianjian Laboratory of Advanced Biomedical Sciences, Institute of Advanced Bio-medical Sciences, Cancer Prevention and Treatment Lab, Zhengzhou University, Zhengzhou, China. AD - China‒US (Henan) Hormel Cancer Institute, Zhengzhou, Henan, China. AD - Department of Pathophysiology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China. FAU - Wang, Keke AU - Wang K AD - China‒US (Henan) Hormel Cancer Institute, Zhengzhou, Henan, China. FAU - Wang, Nan AU - Wang N AD - Department of Radiation Oncology, the Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital, Zhengzhou, Henan, China. FAU - Zhang, Jiachen AU - Zhang J AD - State Key Laboratory of Metabolic Dysregulation & the Prevention and Treatment of Esophageal Cancer; The School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China. AD - China‒US (Henan) Hormel Cancer Institute, Zhengzhou, Henan, China. AD - Department of Pathophysiology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China. FAU - Liu, Kangdong AU - Liu K AUID- ORCID: 0000-0002-4425-5625 AD - State Key Laboratory of Metabolic Dysregulation & the Prevention and Treatment of Esophageal Cancer; The School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China. AD - Tianjian Laboratory of Advanced Biomedical Sciences, Institute of Advanced Bio-medical Sciences, Cancer Prevention and Treatment Lab, Zhengzhou University, Zhengzhou, China. AD - China‒US (Henan) Hormel Cancer Institute, Zhengzhou, Henan, China. AD - Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, Henan, China. AD - The Collaborative Innovation Center of Henan Province for Cancer Chemoprevention, Zhengzhou, Henan, China. AD - State Key Laboratory of Esophageal Cancer Prevention and Treatment, Cancer Prevention and Treatment Lab, Zhengzhou University, Zhengzhou, China. FAU - Li, Xiang AU - Li X AD - State Key Laboratory of Metabolic Dysregulation & the Prevention and Treatment of Esophageal Cancer; The School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China. lixiang@zzu.edu.cn. AD - Tianjian Laboratory of Advanced Biomedical Sciences, Institute of Advanced Bio-medical Sciences, Cancer Prevention and Treatment Lab, Zhengzhou University, Zhengzhou, China. lixiang@zzu.edu.cn. AD - China‒US (Henan) Hormel Cancer Institute, Zhengzhou, Henan, China. lixiang@zzu.edu.cn. AD - Department of Pathophysiology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China. lixiang@zzu.edu.cn. AD - The Collaborative Innovation Center of Henan Province for Cancer Chemoprevention, Zhengzhou, Henan, China. lixiang@zzu.edu.cn. AD - State Key Laboratory of Esophageal Cancer Prevention and Treatment, Cancer Prevention and Treatment Lab, Zhengzhou University, Zhengzhou, China. lixiang@zzu.edu.cn. FAU - Dong, Zigang AU - Dong Z AUID- ORCID: 0000-0002-4174-4028 AD - State Key Laboratory of Metabolic Dysregulation & the Prevention and Treatment of Esophageal Cancer; The School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China. dongzg@zzu.edu.cn. AD - Tianjian Laboratory of Advanced Biomedical Sciences, Institute of Advanced Bio-medical Sciences, Cancer Prevention and Treatment Lab, Zhengzhou University, Zhengzhou, China. dongzg@zzu.edu.cn. AD - China‒US (Henan) Hormel Cancer Institute, Zhengzhou, Henan, China. dongzg@zzu.edu.cn. AD - Department of Pathophysiology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China. dongzg@zzu.edu.cn. AD - The Collaborative Innovation Center of Henan Province for Cancer Chemoprevention, Zhengzhou, Henan, China. dongzg@zzu.edu.cn. AD - State Key Laboratory of Esophageal Cancer Prevention and Treatment, Cancer Prevention and Treatment Lab, Zhengzhou University, Zhengzhou, China. dongzg@zzu.edu.cn. LA - eng GR - 252102521083/Natural Science Foundation of Henan Province (Henan Province Natural Science Foundation)/ GR - 252102520090/Natural Science Foundation of Henan Province (Henan Province Natural Science Foundation)/ GR - 82172996/National Natural Science Foundation of China (National Science Foundation of China)/ PT - Journal Article PT - Review DEP - 20261030 PL - England TA - Signal Transduct Target Ther JT - Signal transduction and targeted therapy JID - 101676423 SB - IM MH - Humans MH - *Cancer-Associated Fibroblasts/pathology/metabolism/immunology MH - *Tumor Microenvironment/genetics/immunology MH - *Neoplasms/pathology/genetics/therapy/immunology MH - Extracellular Matrix/genetics/pathology MH - Animals MH - *Neovascularization, Pathologic/genetics/pathology/immunology/therapy MH - Signal Transduction COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:15 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/03/12 00:00 [received] PHST- 2026/04/13 00:00 [accepted] PHST- 2025/10/20 00:00 [revised] PHST- 2026/10/30 00:15 [entrez] AID - 10.1038/s41392-026-02743-z [pii] AID - 10.1038/s41392-026-02743-z [doi] PST - epublish SO - Signal Transduct Target Ther. 2026 Oct 30;11(1):476. doi: 10.1038/s41392-026-02743-z. PMID- 42957651 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2041-4889 (Electronic) VI - 17 IP - 1 DP - 2026 Aug 1 TI - Targeted degradation of P-TEFb via MDM2 substrate substitution collapses oncogenic transcriptional programs in MDM2-amplified cancers. LID - 920 [pii] LID - 10.1038/s41419-026-09134-w [doi] AB - MDM2 is an oncogenic E3 ubiquitin ligase best known for targeting the tumor suppressor p53 and is frequently amplified in human cancers, including dedifferentiated liposarcoma (DDLPS). We show that supraphysiological MDM2 expression can be exploited to reprogram its ligase activity toward selective degradation of oncogenic transcriptional machinery. Using a substrate substitution strategy, we developed an MDM2-recruiting degrader dCDK9-010 that targets positive transcription elongation factor b, inducing its proteasomal degradation while simultaneously stabilizing p53. This dual action disrupts RNA polymerase II abundance and elongation, preferentially impairing enhancer-driven transcriptional programs that sustain tumor growth. In DDLPS models, this approach produces potent antitumor activity with favorable pharmacokinetic and safety profiles, triggers apoptosis, and enhances macrophage-mediated tumor cell clearance. These findings establish substrate substitution-based reprogramming of MDM2 as a generalizable targeted protein degradation strategy and identify MDM2 amplification as a predictive biomarker for therapeutic response across cancer types, with particular relevance to liposarcoma. CI - (c) 2026. The Author(s). FAU - Chen, Ye AU - Chen Y AUID- ORCID: 0000-0003-0357-1434 AD - Department of Surgical Oncology, and Pediatric Cancer Research Center, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Children and Adolescents' Health and Diseases, Hangzhou, China. chenyephd@zju.edu.cn. AD - Cancer Center, Zhejiang University, Hangzhou, China. chenyephd@zju.edu.cn. FAU - Xie, Long AU - Xie L AD - Department of Surgical Oncology, and Pediatric Cancer Research Center, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Children and Adolescents' Health and Diseases, Hangzhou, China. AD - Institute of Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China. FAU - Guan, Xian AU - Guan X AD - Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education) of the Second Affiliated Hospital and Institute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou, China. FAU - Huang, Xingze AU - Huang X AD - Institute of Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China. FAU - Liu, Jiangyu AU - Liu J AD - Department of Surgical Oncology, and Pediatric Cancer Research Center, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Children and Adolescents' Health and Diseases, Hangzhou, China. FAU - Zhu, Zhipeng AU - Zhu Z AD - Institute of Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China. FAU - Wu, Yuanfang AU - Wu Y AD - Department of Surgical Oncology, and Pediatric Cancer Research Center, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Children and Adolescents' Health and Diseases, Hangzhou, China. FAU - Guo, Hanjun AU - Guo H AD - Department of Surgical Oncology, and Pediatric Cancer Research Center, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Children and Adolescents' Health and Diseases, Hangzhou, China. AD - Institute of Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China. FAU - Ma, Lin AU - Ma L AD - Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education) of the Second Affiliated Hospital and Institute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou, China. FAU - Zhang, Ying AU - Zhang Y AD - Institute of Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China. FAU - Zhou, Jiawei AU - Zhou J AD - Department of Surgical Oncology, and Pediatric Cancer Research Center, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Children and Adolescents' Health and Diseases, Hangzhou, China. FAU - Zheng, Suya AU - Zheng S AD - Department of Surgical Oncology, and Pediatric Cancer Research Center, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Children and Adolescents' Health and Diseases, Hangzhou, China. FAU - Zhou, Ruhong AU - Zhou R AUID- ORCID: 0000-0001-8624-5591 AD - Cancer Center, Zhejiang University, Hangzhou, China. rhzhou@zju.edu.cn. AD - Institute of Biophysics, College of Life Sciences, Zhejiang University, Hangzhou, China. rhzhou@zju.edu.cn. AD - Shanghai Institute for Advanced Study, Zhejiang University, Shanghai, China. rhzhou@zju.edu.cn. AD - Institute of Quantitative Biology, Zhejiang University, Hangzhou, China. rhzhou@zju.edu.cn. FAU - Han, Xin AU - Han X AD - Cancer Center, Zhejiang University, Hangzhou, China. xinhan@zju.edu.cn. AD - Cancer Institute (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education) of the Second Affiliated Hospital and Institute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou, China. xinhan@zju.edu.cn. FAU - Xu, Liang AU - Xu L AUID- ORCID: 0000-0002-2700-4840 AD - Department of Surgical Oncology, and Pediatric Cancer Research Center, Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Children and Adolescents' Health and Diseases, Hangzhou, China. xuliang.phd@zju.edu.cn. AD - Cancer Center, Zhejiang University, Hangzhou, China. xuliang.phd@zju.edu.cn. AD - Institute of Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China. xuliang.phd@zju.edu.cn. AD - Department of Radiation Oncology (Key Laboratory of Cancer Prevention and Intervention, China National Ministry of Education), The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. xuliang.phd@zju.edu.cn. LA - eng GR - 32270746/National Natural Science Foundation of China (National Science Foundation of China)/ GR - 82203247/National Natural Science Foundation of China (National Science Foundation of China)/ GR - 82203415/National Natural Science Foundation of China (National Science Foundation of China)/ GR - LZ23C060002/Natural Science Foundation of Zhejiang Province (Zhejiang Provincial Natural Science Foundation)/ GR - LZ24H160004/Natural Science Foundation of Zhejiang Province (Zhejiang Provincial Natural Science Foundation)/ PT - Journal Article DEP - 20260801 PL - England TA - Cell Death Dis JT - Cell death & disease JID - 101524092 RN - EC 2.3.2.27 (Proto-Oncogene Proteins c-mdm2) RN - EC 2.3.2.27 (MDM2 protein, human) RN - EC 2.7.11.- (Positive Transcriptional Elongation Factor B) RN - 0 (Tumor Suppressor Protein p53) SB - IM MH - *Proto-Oncogene Proteins c-mdm2/metabolism/genetics MH - Humans MH - Animals MH - *Positive Transcriptional Elongation Factor B/metabolism/genetics MH - Cell Line, Tumor MH - Proteolysis/drug effects MH - Tumor Suppressor Protein p53/metabolism MH - *Transcription, Genetic MH - Mice MH - Liposarcoma/genetics/pathology/metabolism COIS- Competing interests: Zhejiang University has filed a patent application on these P-TEFb degraders. X Han, L Xu, Y Chen, X Guan, and J Zhou are co-inventors on this patent application (Reference number: 202510478984.2). The authors declare no competing interests. Ethics approval: All animal experiments were conducted in accordance with ethical approval from Zhejiang University Institutional Animal Care and Use Committees (IACUC Protocol ID: ZJU20210146, ZJU20241058). All methods were performed in accordance with the relevant guidelines and regulations. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:15 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/01/28 00:00 [received] PHST- 2026/07/24 00:00 [accepted] PHST- 2026/07/09 00:00 [revised] PHST- 2026/10/30 00:15 [entrez] AID - 10.1038/s41419-026-09134-w [pii] AID - 10.1038/s41419-026-09134-w [doi] PST - epublish SO - Cell Death Dis. 2026 Aug 1;17(1):920. doi: 10.1038/s41419-026-09134-w. PMID- 42957649 OWN - NLM STAT- Publisher LR - 20261030 IS - 1875-9270 (Electronic) IS - 1051-9815 (Linking) DP - 2026 Oct 29 TI - The precarity of work and life: How insecurity equalizes and stratifies women's experiences. PG - 10519815261502404 LID - 10.1177/10519815261502404 [doi] AB - BackgroundInformal women workers experience persistent employment insecurity due to unstable jobs, low wages, and the absence of social protection. These challenges are often intensified by gender, caste, and socioeconomic inequalities, yet their lived experiences of these females remain underexplored.ObjectiveThis Work examines how precarious work shapes the everyday lives of informal women workers in rural Bihar, India, focusing on how insecurity creates both shared experiences and unequal outcomes while exploring women's strategies for coping and resilience.MethodsA qualitative case study design was employed using 15 semi-structured, face-to-face interviews with women engaged in agricultural wage labor, dairy-related work, domestic service, and other informal work. Contributors were purposively selected through self-help groups, dairy cooperatives, and community networks. Data collection from August 2025 to December 2025 was transliterated, explained into English, and analyzed using thematic content analysis.ResultsFour key themes emerged: widespread employment insecurity characterized by irregular work and income; the influence of intersecting social inequalities, particularly caste and gender, on workers' experiences; the physical, emotional, and economic burdens of precarious employment; and the importance of informal support networks, self-help groups, and collective resilience in managing everyday challenges. Although precarity was a common experience, its effects varied significantly according to women's social and economic positions.ConclusionsPremature work is not just about job insecurity but has been defined as a multifaceted phenomenon based on structural inequalities. Efforts to ensure social security, worker rights, and empowerment of informal women workers need to be based on an intersectional perspective due to the complexities surrounding women's work experiences. FAU - Kumari, Anshu AU - Kumari A AUID- ORCID: 0009-0002-2252-1141 AD - Department of Humanities and Social Sciences, National Institute of Technology Patna, Bihar, India. RINGGOLD: 230635 FAU - Tiwari, Manish AU - Tiwari M AUID- ORCID: 0000-0002-5653-2415 AD - Department of Humanities and Social Sciences, National Institute of Technology Patna, Bihar, India. RINGGOLD: 230635 LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Work JT - Work (Reading, Mass.) JID - 9204382 SB - IM OTO - NOTNLM OT - intersectionality OT - precarious labour OT - rural Bihar OT - women workers OT - work OT - workplace insecurity EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:14 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/30 00:14 [entrez] AID - 10.1177/10519815261502404 [doi] PST - aheadofprint SO - Work. 2026 Oct 29:10519815261502404. doi: 10.1177/10519815261502404. PMID- 42957648 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2059-3635 (Electronic) IS - 2059-3635 (Linking) VI - 11 IP - 1 DP - 2026 Oct 28 TI - Aromaticity at spike position 456 affects functional immunogenicity of SARS-CoV-2 Omicron variant receptor-binding domain. LID - 496 [pii] LID - 10.1038/s41392-026-02939-3 [doi] AB - The recurrent emergence of the F456L substitution in SARS-CoV-2 Omicron sublineages has been associated with immune evasion, yet its impact on vaccine-induced immune responses remains poorly understood. Using a trimeric RBD-HR subunit vaccine platform, we investigated how residue 456 influences the functional immunogenicity of the receptor-binding domain (RBD). We found that the F456L substitution markedly reduced neutralizing antibody responses against multiple SARS-CoV-2 variants, despite maintaining comparable levels of total antigen-specific IgG, indicating a selective impairment of functional immunogenicity rather than overall antigenicity. Single-cell RNA sequencing and B-cell receptor (BCR) repertoire analyses further revealed that the F456L mutation weakened germinal center responses, reduced clonal expansion, and impaired repertoire remodeling of antigen-specific B cells. Structural modeling, biolayer interferometry, and calcium flux assays demonstrated that aromatic residues at position 456 promote effective germline BCR recognition and activation. Importantly, replacing the non-aromatic leucine residue with aromatic amino acids, including tyrosine or tryptophan, restored the neutralizing antibody-eliciting capacity of the antigen. Together, these findings identify aromaticity at residue 456 as a critical determinant of RBD functional immunogenicity and demonstrate how a single amino acid substitution can reshape B-cell responses and antibody quality. This work provides important insights for rational vaccine antigen design and advances our understanding of how site-specific mutations regulate antiviral immunity. CI - (c) 2026. The Author(s). FAU - Peng, Dandan AU - Peng D AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Chen, Zimin AU - Chen Z AUID- ORCID: 0000-0002-2862-9674 AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Fu, Minyang AU - Fu M AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Hong, Weiqi AU - Hong W AUID- ORCID: 0000-0003-1668-2301 AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Dai, Mengzhe AU - Dai M AUID- ORCID: 0009-0006-5734-2812 AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Zeng, Hao AU - Zeng H AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Lin, Sheng AU - Lin S AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Lei, Hong AU - Lei H AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Yang, Jingyun AU - Yang J AUID- ORCID: 0000-0003-1908-1480 AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Shi, Jie AU - Shi J AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Huang, Xiya AU - Huang X AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Ye, Chunjun AU - Ye C AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Qin, Furong AU - Qin F AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Zhang, Yu AU - Zhang Y AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Hao, Ying AU - Hao Y AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Wang, Binhan AU - Wang B AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Ren, Wenyan AU - Ren W AUID- ORCID: 0000-0001-6333-8496 AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Chen, Li AU - Chen L AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Liu, Jian AU - Liu J AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Alu, Aqu AU - Alu A AUID- ORCID: 0000-0002-9981-0120 AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Lu, Yishan AU - Lu Y AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Du, Xinyi AU - Du X AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Li, Jiong AU - Li J AUID- ORCID: 0000-0002-3742-630X AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Shi, Huashan AU - Shi H AUID- ORCID: 0000-0002-9958-9892 AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Wei, Yuquan AU - Wei Y AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. FAU - Chen, Yi AU - Chen Y AUID- ORCID: 0000-0002-5180-3490 AD - Division of Gastrointestinal Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China. toddy@scu.edu.cn. AD - Department of Infection Control, West China Hospital, Sichuan University, Chengdu, China. toddy@scu.edu.cn. FAU - Lu, Shuaiyao AU - Lu S AUID- ORCID: 0000-0003-1675-9735 AD - National Kunming High-level Biosafety Primate Research Center, Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Yunnan, China. lushuaiyao-km@163.com. FAU - He, Cai AU - He C AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. hecaihc@163.com. FAU - Lu, Guangwen AU - Lu G AUID- ORCID: 0000-0001-7568-592X AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. lugw@scu.edu.cn. FAU - Wei, Xiawei AU - Wei X AUID- ORCID: 0000-0002-6513-6422 AD - Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, People's Republic of China. xiaweiwei@scu.edu.cn. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Signal Transduct Target Ther JT - Signal transduction and targeted therapy JID - 101676423 RN - 0 (Spike Glycoprotein, Coronavirus) RN - 0 (spike protein, SARS-CoV-2) RN - 0 (Antibodies, Neutralizing) RN - 0 (Receptors, Antigen, B-Cell) RN - 0 (Antibodies, Viral) RN - 0 (COVID-19 Vaccines) RN - SARS-CoV-2 variants SB - IM MH - *SARS-CoV-2/immunology/genetics MH - *Spike Glycoprotein, Coronavirus/immunology/genetics/chemistry MH - Humans MH - Animals MH - Antibodies, Neutralizing/immunology MH - Receptors, Antigen, B-Cell/immunology/genetics MH - *COVID-19/immunology/genetics/virology/prevention & control MH - Antibodies, Viral/immunology MH - *COVID-19 Vaccines/immunology/genetics MH - Protein Domains MH - B-Lymphocytes/immunology COIS- Competing interests: Although Xiawei Wei serves on the editorial board of Signal Transduction and Targeted Therapy, she was entirely excluded from the editorial handling and peer-review process for this submission. The remaining authors report no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:14 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/11/25 00:00 [received] PHST- 2026/07/09 00:00 [accepted] PHST- 2026/06/01 00:00 [revised] PHST- 2026/10/30 00:14 [entrez] AID - 10.1038/s41392-026-02939-3 [pii] AID - 10.1038/s41392-026-02939-3 [doi] PST - epublish SO - Signal Transduct Target Ther. 2026 Oct 28;11(1):496. doi: 10.1038/s41392-026-02939-3. PMID- 42957642 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261030 LR - 20261030 IS - 2056-807X (Electronic) IS - 2056-807X (Linking) VI - 12 IP - 1 DP - 2026 Oct 28 TI - Smartphone applications in domestic abuse referral pathways: implications for dental practice. LID - 102 [pii] LID - 10.1038/s41405-026-00501-5 [doi] AB - Domestic abuse is a persistent public health, safeguarding and human rights challenge. In England and Wales, the Crime Survey for England and Wales estimated that approximately 3.8 million people aged 16 years and over experienced domestic abuse in the year ending March 2025. Dentistry has an important role because interpersonal violence frequently affects the face, mouth and teeth, and a dental appointment may provide an opportunity to access healthcare. However, general dental practitioners may be uncertain how to progress from recognising possible abuse to an appropriate safeguarding response, particularly when disclosure is hesitant or incomplete. Smartphone applications can provide information, safety planning, incident recording and routes to specialist support, but their evidence base and safety implications vary. The strongest peer-reviewed evidence among the resources considered relates to myPlan and related safety decision aids, with benefits for selected outcomes that vary by intervention and population. Digital tools should therefore support, rather than replace, professional judgement. This narrative review critically appraises selected digital resources and sets out a conceptual dental safeguarding pathway centred on privacy, sensitive enquiry, objective documentation, risk assessment and proportionate referral or escalation. CI - (c) 2026. The Author(s). FAU - Barakati, Tamana AU - Barakati T AD - Institute of Dentistry, Queen Mary University of London, London, UK. t.barakaty@qmul.ac.uk. AD - The Bloomsbury Trust, London, UK. t.barakaty@qmul.ac.uk. FAU - Coulthard, Paul AU - Coulthard P AUID- ORCID: 0000-0003-1682-0874 AD - Institute of Dentistry, Queen Mary University of London, London, UK. AD - The Bloomsbury Trust, London, UK. AD - University of Jordan, Amman, Jordan. FAU - Holmes, Simon AU - Holmes S AD - Barts Health NHS Trust, London, UK. FAU - Farook, Mohammad Ismail AU - Farook MI AD - Institute of Dentistry, Queen Mary University of London, London, UK. FAU - Dave, Manas AU - Dave M AUID- ORCID: 0000-0002-1676-801X AD - Institute of Dentistry, Queen Mary University of London, London, UK. md39@sanger.ac.uk. AD - University of Manchester, Manchester, UK. md39@sanger.ac.uk. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - BDJ Open JT - BDJ open JID - 101709456 COIS- Competing interests: Manas Dave is an Associate Editor for BDJ Open. He was not involved in the editorial handling, peer review, or editorial decision-making process for this manuscript. The remaining authors declare that they have no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:14 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/08/03 00:00 [received] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/30 00:14 [entrez] AID - 10.1038/s41405-026-00501-5 [pii] AID - 10.1038/s41405-026-00501-5 [doi] PST - epublish SO - BDJ Open. 2026 Oct 28;12(1):102. doi: 10.1038/s41405-026-00501-5. PMID- 42957633 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2041-4889 (Electronic) VI - 17 IP - 1 DP - 2026 Aug 1 TI - SFRP2(+) fibroblasts orchestrate post-chemotherapy remodeling and define a tumor-driven prognostic program, NBTRP, in adrenal neuroblastoma. LID - 915 [pii] LID - 10.1038/s41419-026-09132-y [doi] AB - Neuroblastoma (NB) is a highly heterogeneous pediatric cancer in which chemotherapy resistance and poor prognosis are tightly linked to the tumor microenvironment (TME). Yet, how chemotherapy reshapes stromal components, particularly cancer-associated fibroblasts (CAFs), and the clinical implications of these alterations remain unclear. Here, by integrating single-cell, spatial, and bulk RNA-seq data from nine NB datasets, we systematically characterized chemotherapy-induced TME remodeling. Fibroblast subtypes were defined through clustering, stemness estimation, and functional enrichment analyses, revealing SFRP2(+) inflammatory CAFs (iCAFs) as the dominant fibroblast population enriched after chemotherapy in adrenal NB TME. These SFRP2(+) iCAFs exhibited high expression of SFRP2, FBLN1, and chemokines CXCL2 and CXCL3, and were found to promote angiogenesis by signaling to endothelial cells through the CCL2/CXCL2/3/8-ACKR1 axis. Building on the chemotherapy-associated gene expression changes in adrenal NB tumor cells, we developed a 6-gene prognostic model, termed NBTRP. The NBTRP robustly stratified patient survival across multiple cohorts and was associated with reduced cytotoxic T cell infiltration, increased tumor purity, and distinct drug sensitivity profiles. High NBTRP scores predicted enhanced sensitivity to chemotherapeutic agents such as vinblastine and etoposide, as well as improved response to anti-PD-L1 immunotherapy. Functional validation further identified SERPINF1, the top NBTRP feature, as a key effector that promoted NB cell invasion in vitro and modulated drug responses to vincristine, etoposide, cisplatin and cyclophosphamide. Together, our findings uncover SFRP2(+) iCAFs as pivotal mediators of post-chemotherapy TME remodeling and establish NBTRP and SERPINF1 as clinically relevant biomarkers that bridge tumor-stroma dynamics with prognosis and therapeutic guidance in adrenal neuroblastoma. CI - (c) 2026. The Author(s). FAU - Wu, Jun AU - Wu J AUID- ORCID: 0000-0002-1717-0378 AD - Department of Obstetrics and Gynecology, Department of Paediatrics, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine; The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China. AD - School of Biomedical Science, Hunan University, Changsha, China. FAU - Gu, Jian AU - Gu J AUID- ORCID: 0009-0000-5154-8461 AD - Department of Obstetrics and Gynecology, Department of Paediatrics, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine; The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China. FAU - Li, Guodong AU - Li G AD - Department of Obstetrics and Gynecology, Department of Paediatrics, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine; The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China. FAU - Xu, Ye AU - Xu Y AD - Department of Obstetrics and Gynecology, Department of Paediatrics, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine; The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China. FAU - Li, Weihua AU - Li W AD - Department of Obstetrics and Gynecology, Department of Paediatrics, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine; The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China. FAU - Liao, Yuanxing AU - Liao Y AD - Department of Obstetrics and Gynecology, Department of Paediatrics, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine; The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China. FAU - Li, Chi AU - Li C AD - Department of Pediatric Hematology and Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. FAU - Zhang, Wei AU - Zhang W AUID- ORCID: 0009-0007-6333-4138 AD - Institute for Brain Research and Rehabilitation, South China Normal University, Guangzhou, China. FAU - Wang, Dao AU - Wang D AUID- ORCID: 0000-0001-8426-1467 AD - Department of Pediatric Hematology and Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China. deai315@163.com. FAU - Xie, Lichun AU - Xie L AUID- ORCID: 0000-0002-6183-5904 AD - Department of Obstetrics and Gynecology, Department of Paediatrics, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine; The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, China. xielichunst@sina.com. LA - eng PT - Journal Article DEP - 20260801 PL - England TA - Cell Death Dis JT - Cell death & disease JID - 101524092 RN - 0 (Membrane Proteins) RN - 0 (Secreted Frizzled-Related Proteins) RN - 0 (SFRP2 protein, human) SB - IM MH - Humans MH - *Neuroblastoma/drug therapy/pathology/genetics/metabolism MH - Prognosis MH - *Cancer-Associated Fibroblasts/metabolism/pathology/drug effects MH - *Membrane Proteins/metabolism/genetics MH - Secreted Frizzled-Related Proteins MH - Tumor Microenvironment/drug effects MH - *Adrenal Gland Neoplasms/drug therapy/pathology/metabolism/genetics MH - Gene Expression Regulation, Neoplastic/drug effects MH - Animals MH - Cell Line, Tumor MH - Neovascularization, Pathologic COIS- Competing interests: The authors declare no competing interests. Ethics approval and consent to participate: All human tissue samples and cell lines used in this study were collected in accordance with institutional guidelines and the Declaration of Helsinki. Ethical approval was obtained from the Ethics Committee of the Third Affiliated Hospital of Guangzhou Medical University (Approval No. 2025302). EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:13 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/12/17 00:00 [received] PHST- 2026/07/23 00:00 [accepted] PHST- 2026/07/16 00:00 [revised] PHST- 2026/10/30 00:13 [entrez] AID - 10.1038/s41419-026-09132-y [pii] AID - 10.1038/s41419-026-09132-y [doi] PST - epublish SO - Cell Death Dis. 2026 Aug 1;17(1):915. doi: 10.1038/s41419-026-09132-y. PMID- 42957629 OWN - NLM STAT- MEDLINE DCOM- 20261030 LR - 20261030 IS - 2041-4889 (Electronic) VI - 17 IP - 1 DP - 2026 Aug 6 TI - Constitutive androgen receptor activity sustains DNA replication programs in triple-negative breast cancer. LID - 917 [pii] LID - 10.1038/s41419-026-09180-4 [doi] AB - Triple-negative breast cancer (TNBC) is an aggressive subtype lacking effective targeted therapies. Despite AR expression in nearly half of TNBCs, androgen receptor signaling inhibitors (ARSIs) have shown minimal clinical benefit. In this study, we utilized HP518, a potent PROTAC that selectively degrades AR, to interrogate the role of AR in TNBC. We uncover a non-canonical, androgen-independent function of AR in AR-positive TNBC cells: AR cooperates with transcriptional regulators FoxA1 and GATA3 to promote expression of DNA replication genes critical for tumor proliferation. Genetic and pharmacological degradation of AR suppresses tumor cell growth, downregulates key DNA replication genes, and disrupts AR-associated transcriptional complexes. In AR-positive TNBC patient-derived xenografts (PDX), HP518 robustly inhibits tumor growth and synergizes with PI3Kalpha inhibition and chemotherapy. Our findings identify a previously unappreciated mechanism of AR action in TNBC and support AR degradation-not antagonism as a rational therapeutic strategy. CI - (c) 2026. The Author(s). FAU - Li, Jing AU - Li J AD - Hinova Pharmaceuticals Inc, Chengdu, China. FAU - Yuan, Hongwei AU - Yuan H AD - Hinova Pharmaceuticals Inc, Chengdu, China. FAU - Chen, Kefan AU - Chen K AD - Hinova Pharmaceuticals Inc, Chengdu, China. FAU - Peng, Jian AU - Peng J AD - Hinova Pharmaceuticals Inc, Chengdu, China. FAU - Du, Wu AU - Du W AD - Hinova Pharmaceuticals Inc, Chengdu, China. FAU - Li, Xinghai AU - Li X AUID- ORCID: 0000-0003-4493-115X AD - Hinova Pharmaceuticals Inc, Chengdu, China. xhli@hinovapharma.com. LA - eng PT - Journal Article DEP - 20260806 PL - England TA - Cell Death Dis JT - Cell death & disease JID - 101524092 RN - 0 (Receptors, Androgen) RN - 0 (Hepatocyte Nuclear Factor 3-alpha) RN - 0 (Proteolysis Targeting Chimera) RN - 0 (FOXA1 protein, human) RN - 0 (AR protein, human) RN - 0 (GATA3 Transcription Factor) RN - 0 (Androgen Receptor Antagonists) SB - IM MH - *Triple Negative Breast Neoplasms/genetics/metabolism/pathology/drug therapy MH - Humans MH - *Receptors, Androgen/metabolism/genetics MH - Female MH - Animals MH - *DNA Replication/drug effects/genetics MH - Cell Line, Tumor MH - Hepatocyte Nuclear Factor 3-alpha/metabolism/genetics MH - Cell Proliferation/drug effects MH - Proteolysis Targeting Chimera MH - Mice MH - Gene Expression Regulation, Neoplastic/drug effects MH - GATA3 Transcription Factor/metabolism/genetics MH - Signal Transduction/drug effects MH - Xenograft Model Antitumor Assays MH - Androgen Receptor Antagonists/pharmacology COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:31 CRDT- 2026/10/30 00:13 PHST- 2026/10/30 05:31 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2025/09/10 00:00 [received] PHST- 2026/07/29 00:00 [accepted] PHST- 2026/07/20 00:00 [revised] PHST- 2026/10/30 00:13 [entrez] AID - 10.1038/s41419-026-09180-4 [pii] AID - 10.1038/s41419-026-09180-4 [doi] PST - epublish SO - Cell Death Dis. 2026 Aug 6;17(1):917. doi: 10.1038/s41419-026-09180-4. PMID- 42957622 OWN - NLM STAT- Publisher LR - 20261030 IS - 1497-0015 (Electronic) IS - 0706-7437 (Linking) DP - 2026 Oct 29 TI - Machine Learning in the Differential Diagnosis of Bipolar and Borderline Personality Disorders: A Scoping Review. PG - 7067437261500228 LID - 10.1177/07067437261500228 [doi] AB - BackgroundBipolar disorder (BD) and borderline personality disorder (BPD) are associated with substantial morbidity and can be difficult to distinguish when symptoms are assessed cross-sectionally. This scoping review examines machine learning (ML) and related predictive approaches designed to differentiate the two conditions.MethodsWe searched Ovid MEDLINE, APA PsycInfo, Embase, Web of Science, Scopus, IEEE Xplore, ACM Digital Library, and selected computer science conference proceedings through July 2, 2026. Full-text peer-reviewed journal articles and conference papers were eligible when they used a data-driven computational or statistical prediction approach to distinguish, classify, or characterize BD and BPD. Of 2,594 records identified, 2,523 unique records were screened, and 24 full texts were assessed. Screening followed the Population, Concept, Context framework, Joanna Briggs Institute guidance, and preferred reporting items for systematic reviews and meta-analyses extension for scoping reviews.ResultsNine reports met the inclusion criteria. Seven participant-based reports contributed an aggregate of 986 report-level participants, although two reports used overlapping cohorts; one conference paper used synthetic data, and one study analyzed 149,798 Reddit posts. Studies including clinical samples reported BD versus BPD discrimination AUC (area under the receiver operating characteristic curve) values of 0.67 to 0.91 and accuracies ranging from 61.7% to 90%. These estimates were not directly comparable and were derived from small, often imbalanced samples using heterogeneous internal validation. No model was externally validated in an independent clinical cohort.ConclusionThe evidence remains preliminary and does not support clinical deployment. Reported performance is constrained by small samples, class imbalance, uncertain ground-truth labels, spectrum bias, and reliance on pre-existing diagnoses. Prospective studies in consecutive at-risk cohorts, with transparent reporting and independent external validation, are required. FAU - Dargel, Aroldo AU - Dargel A AUID- ORCID: 0009-0004-2047-6361 AD - Department of Psychiatry, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada. RINGGOLD: 27337 AD - Precision Psychiatry and Artificial Intelligence for Early Intervention and System Innovation in Affective Disorders (PRECISAI) Lab, Department of Psychiatry, University of Ottawa, Ottawa, ON, Canada. RINGGOLD: 6363 AD - Department of Mental Health, The Ottawa Hospital, Ottawa, ON, Canada. AD - Neuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada. RINGGOLD: 10055 AD - Department of Psychiatry, CHEO and CHEO Research Institute, Ottawa, ON, Canada. RINGGOLD: 274065 FAU - Tanya, Tanya AU - Tanya T AD - Precision Psychiatry and Artificial Intelligence for Early Intervention and System Innovation in Affective Disorders (PRECISAI) Lab, Department of Psychiatry, University of Ottawa, Ottawa, ON, Canada. RINGGOLD: 6363 AD - Department of Mental Health, The Ottawa Hospital, Ottawa, ON, Canada. FAU - Alhadhrami, Asma AU - Alhadhrami A AD - Precision Psychiatry and Artificial Intelligence for Early Intervention and System Innovation in Affective Disorders (PRECISAI) Lab, Department of Psychiatry, University of Ottawa, Ottawa, ON, Canada. RINGGOLD: 6363 AD - Faculty of Medicine, Khalifa University, Abu Dhabi, United Arab Emirates. RINGGOLD: 105955 FAU - Mahdiabadi, Sara AU - Mahdiabadi S AD - Precision Psychiatry and Artificial Intelligence for Early Intervention and System Innovation in Affective Disorders (PRECISAI) Lab, Department of Psychiatry, University of Ottawa, Ottawa, ON, Canada. RINGGOLD: 6363 AD - Clinical Science and Translational Medicine Program, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada. RINGGOLD: 6363 FAU - Shorr, Risa AU - Shorr R AD - Precision Psychiatry and Artificial Intelligence for Early Intervention and System Innovation in Affective Disorders (PRECISAI) Lab, Department of Psychiatry, University of Ottawa, Ottawa, ON, Canada. RINGGOLD: 6363 AD - Department of Mental Health, The Ottawa Hospital, Ottawa, ON, Canada. FAU - Morris, Caitlin AU - Morris C AD - Precision Psychiatry and Artificial Intelligence for Early Intervention and System Innovation in Affective Disorders (PRECISAI) Lab, Department of Psychiatry, University of Ottawa, Ottawa, ON, Canada. RINGGOLD: 6363 AD - Department of Life Sciences, Queen's University, Kingston, ON, Canada. RINGGOLD: 4257 FAU - Pajer, Kathleen AU - Pajer K AD - Department of Psychiatry, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada. RINGGOLD: 27337 AD - Department of Psychiatry, CHEO and CHEO Research Institute, Ottawa, ON, Canada. RINGGOLD: 274065 FAU - Imbault, Marion AU - Imbault M AUID- ORCID: 0000-0001-7742-6307 AD - Precision Psychiatry and Artificial Intelligence for Early Intervention and System Innovation in Affective Disorders (PRECISAI) Lab, Department of Psychiatry, University of Ottawa, Ottawa, ON, Canada. RINGGOLD: 6363 AD - Bicetre Hospital, Mood Center Paris-Saclay, DMU Neurosciences, Universite Paris-Saclay, Assistance Publique-Hopitaux de Paris (AP-HP), Le Kremlin-Bicetre, Paris, France. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - United States TA - Can J Psychiatry JT - Canadian journal of psychiatry. Revue canadienne de psychiatrie JID - 7904187 SB - IM OTO - NOTNLM OT - Trouble bipolaire OT - apprentissage machine OT - artificial intelligence OT - bipolar disorder OT - borderline personality disorder OT - examen de la portee. OT - intelligence artificielle OT - machine learning OT - modeles predictifs OT - predictive models OT - scoping review OT - trouble de la personnalite limite EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/30 00:13 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/10/30 00:13 [entrez] AID - 10.1177/07067437261500228 [doi] PST - aheadofprint SO - Can J Psychiatry. 2026 Oct 29:7067437261500228. doi: 10.1177/07067437261500228. PMID- 42957617 OWN - NLM STAT- Publisher LR - 20261030 IS - 2328-9503 (Electronic) IS - 2328-9503 (Linking) DP - 2026 Oct 29 TI - Improving the Diagnostic Accuracy of Plasma Alzheimer's Disease Biomarkers With Objective and Subjective Cognitive Screening. LID - 10.1002/acn3.70565 [doi] AB - BACKGROUND: Blood-based biomarkers are increasingly used to identify Alzheimer's disease (AD) pathology and may play an important role in early disease screening and participant selection for clinical trials. However, their performance across stages of cognitive impairment remains unclear. This study examined whether plasma biomarkers differ in their ability to predict amyloid pathology by performance on cognitive screening measures. METHODS: Participants were older adults from the community-based Vanderbilt Memory and Aging Project (N = 154; mean age = 72 +/- 6 years; 37% MCI). Plasma phosphorylated tau (p-tau)(231) and glial fibrillary acidic protein (GFAP) were measured using Quanterix Simoa technology. Amyloid positivity was defined by cerebrospinal fluid beta-amyloid 42/40 ratio < 0.072. Objective cognitive impairment was defined as /=4.0 was independently associated with inferior overall survival (derivation: hazard ratio, 1.86; 95% confidence interval, 1.40-2.45; validation: hazard ratio, 1.53; 95% confidence interval, 1.13-2.07). Combining maximum standardized uptake value (>/=4.0 vs <4.0) with post-neoadjuvant therapy carbohydrate antigen 19-9 (>37 vs /=4.0 modestly predicted early recurrence within 6 months (area under the curve, 0.65), whereas a "double-positive" rule (maximum standardized uptake value >/=4.0 and carbohydrate antigen 19-9 >37 U/mL) increased specificity (0.83) with lower sensitivity (0.42), with 32% early recurrence within 6 months in this subgroup. A high maximum standardized uptake value was associated with distant but not margin-related failure. CONCLUSION: A post-neoadjuvant therapy maximum standardized uptake value threshold of 4.0 defined a reproducible, single-time-point metabolic criterion that complemented carbohydrate antigen 19-9 level to enable biology-guided surgical selection in anatomically resectable pancreatic ductal adenocarcinoma. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Inc. All rights reserved. FAU - Ikenaga, Naoki AU - Ikenaga N AD - Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. Electronic address: ikenaga.naoki.533@m.kyushu-u.ac.jp. FAU - Kim, Hyung Sun AU - Kim HS AD - Division of Hepato-biliary-pancreas Surgery, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea. Electronic address: milk8508@yuhs.ac. FAU - Baba, Shingo AU - Baba S AD - Division of Medical Quantum Science, Department of Health Sciences, Faculty of Medicine, Kyushu University, Fukuoka, Japan. FAU - Chae, Yoon Soo AU - Chae YS AD - Department of Surgery and Cancer Research Institute, Seoul National University College of Medicine, Seoul, South Korea. FAU - Kwon, Wooil AU - Kwon W AD - Department of Surgery and Cancer Research Institute, Seoul National University College of Medicine, Seoul, South Korea. FAU - Jang, Jin-Young AU - Jang JY AD - Department of Surgery and Cancer Research Institute, Seoul National University College of Medicine, Seoul, South Korea. FAU - Makino, Isamu AU - Makino I AD - Department of Hepato-Biliary-Pancreatic Surgery and Transplantation, Kanazawa University, Kanazawa, Japan. FAU - Yagi, Shintaro AU - Yagi S AD - Department of Hepato-Biliary-Pancreatic Surgery and Transplantation, Kanazawa University, Kanazawa, Japan. FAU - Ohgi, Katsuhisa AU - Ohgi K AD - Division of Hepato-Biliary-Pancreatic Surgery, Shizuoka Cancer Center, Shizuoka, Japan. FAU - Sugiura, Teiichi AU - Sugiura T AD - Division of Hepato-Biliary-Pancreatic Surgery, Shizuoka Cancer Center, Shizuoka, Japan. FAU - Tomioka, Atsushi AU - Tomioka A AD - Department of General and Gastroenterological Surgery, Osaka Medical and Pharmaceutical University, Takatsuki, Japan. FAU - Asakuma, Mitsuhiro AU - Asakuma M AD - Department of General and Gastroenterological Surgery, Osaka Medical and Pharmaceutical University, Takatsuki, Japan. FAU - Oshima, Minoru AU - Oshima M AD - Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, Kagawa, Japan. FAU - Okano, Keiichi AU - Okano K AD - Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, Kagawa, Japan. FAU - Lee, Boram AU - Lee B AD - Department of Surgery, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, South Korea. FAU - Yoon, Yoo-Seok AU - Yoon YS AD - Department of Surgery, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, South Korea. FAU - Watanabe, Toru AU - Watanabe T AD - Department of Surgery and Science, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan. FAU - Eguchi, Hidetoshi AU - Eguchi H AD - Department of Gastroenterological Surgery, Graduate School of Medicine, The University of Osaka, Osaka, Japan. FAU - Takami, Hideki AU - Takami H AD - Department of Surgery, Nagoya University Hospital, Nagoya, Japan. FAU - Idichi, Tetsuya AU - Idichi T AD - Department of Digestive Surgery, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan. FAU - Hatano, Etsuro AU - Hatano E AD - Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan. FAU - Tanaka, Masayuki AU - Tanaka M AD - Department of Surgery, Keio University School of Medicine, Tokyo, Japan. FAU - Marubashi, Shigeru AU - Marubashi S AD - Department of Hepato-Biliary-Pancreatic and Transplant Surgery, Fukushima Medical University, Fukushima, Japan. FAU - Kim, Hongbeom AU - Kim H AD - Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea. FAU - Hirano, Satoshi AU - Hirano S AD - Department of Gastroenterological Surgery II, Hokkaido University Faculty of Medicine, Sapporo, Japan. FAU - Seo, Hyung Il AU - Seo HI AD - Department of Surgery, Pusan National University School of Medicine, Biomedical Research Institute, Pusan National University Hospital, Busan, South Korea. FAU - Chong, Eui Hyuk AU - Chong EH AD - Division of Hepatobiliary and Pancreas, Department of Surgery, CHA Bundang Medical Center, CHA University, Seongnam, South Korea. FAU - Kim, Naru AU - Kim N AD - Department of Surgery, Ewha Womans University Mokdong Hospital, Ewha Womans University College of Medicine, Seoul, South Korea. FAU - Lee, Jung Min AU - Lee JM AD - Department of Surgery, Dongtan Sacred Heart Hospital, Hallym University, Hwaseong, South Korea. FAU - Choi, Munseok AU - Choi M AD - Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, Yongin Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea. FAU - Kosaka, Hisashi AU - Kosaka H AD - Department of Hepatobiliary Surgery, Kansai Medical University, Hirakata, Japan. FAU - Fujii, Tsutomu AU - Fujii T AD - Department of Surgery and Science, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan; Japanese Society of Hepato-Biliary-Pancreatic Surgery, Tokyo, Japan. FAU - Ishigami, Kousei AU - Ishigami K AD - Department of Clinical Radiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. FAU - Nakamura, Masafumi AU - Nakamura M AD - Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan; Japanese Society of Hepato-Biliary-Pancreatic Surgery, Tokyo, Japan. FAU - Ohtsuka, Masayuki AU - Ohtsuka M AD - Japanese Society of Hepato-Biliary-Pancreatic Surgery, Tokyo, Japan; Department of General Surgery, Graduate School of Medicine, Chiba University, Chiba, Japan. LA - eng PT - Journal Article DEP - 20260803 PL - United States TA - Surgery JT - Surgery JID - 0417347 SB - IM COIS- Conflict of Interest/Disclosure The authors declare no conflicts of interest. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/29 23:02 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/02/11 00:00 [received] PHST- 2026/07/03 00:00 [revised] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/29 23:02 [entrez] AID - S0039-6060(26)00428-9 [pii] AID - 10.1016/j.surg.2026.110503 [doi] PST - aheadofprint SO - Surgery. 2026 Aug 3:110503. doi: 10.1016/j.surg.2026.110503. PMID- 42957516 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-291X (Electronic) IS - 0301-5629 (Linking) DP - 2026 Oct 29 TI - Microwave Ablation for Hepatocellular Carcinoma in Patients Older Than 60 Years: Impact of Comorbidities on Survival. LID - S0301-5629(26)00400-X [pii] LID - 10.1016/j.ultrasmedbio.2026.09.024 [doi] AB - OBJECTIVE: To examine whether comorbidity burden, categorized using the modified Charlson Comorbidity Index (mCCI), was associated with overall survival (OS), progression-free survival (PFS), and cancer-related death after ultrasound-guided microwave ablation (MWA) in older patients with hepatocellular carcinoma (HCC). METHODS: This multicenter retrospective cohort included 530 patients aged 60 years or older with Barcelona Clinic Liver Cancer stage A or B HCC. Patients were classified as mCCI 0, 1, or >/= 2. Generalized propensity scores were estimated using multinomial logistic regression. Overlap-weighted Cox models evaluated OS and PFS, with stabilized inverse probability of treatment weighting and bootstrap validation as sensitivity analyses. Fine-Gray models evaluated cancer-related death, with noncancer death as a competing event. RESULTS: Overall, 301 patients had mCCI 0, 107 had mCCI 1, and 122 had mCCI >/= 2. Overlap weighting achieved standardized mean differences below 0.10. No overall association was observed between mCCI and OS (global p = 0.103) or PFS (global p = 0.973). Among 153 deaths, 111 were cancer related and 42 were noncancer deaths. Fine-Gray analysis showed no association between mCCI and cancer-related death (global p = 0.750). Sensitivity analyses were consistent. CONCLUSION: mCCI was not robustly associated with OS, PFS, or cancer-related death among selected older patients with HCC treated with MWA. CI - Copyright (c) 2026 World Federation for Ultrasound in Medicine and Biology. Published by Elsevier Inc. All rights reserved. FAU - He, Liting AU - He L AD - Department of Interventional Ultrasound, the First Medical Center of Chinese PLA General Hospital, Beijing, China; The Third Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Qiao, Yannan AU - Qiao Y AD - Department of Interventional Ultrasound, the First Medical Center of Chinese PLA General Hospital, Beijing, China; China-Japan Friendship Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Chen, Sitong AU - Chen S AD - Department of Interventional Ultrasound, the First Medical Center of Chinese PLA General Hospital, Beijing, China. FAU - Luo, Ting AU - Luo T AD - Department of Interventional Ultrasound, the First Medical Center of Chinese PLA General Hospital, Beijing, China. FAU - Zheng, Lin AU - Zheng L AD - Department of Interventional Ultrasound, the First Medical Center of Chinese PLA General Hospital, Beijing, China. FAU - Zhang, Dezhi AU - Zhang D AD - Abdominal Ultrasound Department, the First Hospital of Jilin University, Changchun, China. FAU - Wu, Songsong AU - Wu S AD - Department of Ultrasonography, Shengli Clinical Medical College of Fujian Medical University, Fuzhou, China. FAU - He, Guang-Bin AU - He GB AD - Department of Ultrasound, Xijing Hospital, the Fourth Military Medical University, Xian, China. FAU - Yang, Hong AU - Yang H AD - Department of Ultrasound, the First Affiliated Hospital of Guangxi Medical University, Nanning, China. FAU - Li, Xiao AU - Li X AD - Department of Interventional Therapy, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. FAU - Yu, Jie AU - Yu J AD - Department of Interventional Ultrasound, the First Medical Center of Chinese PLA General Hospital, Beijing, China. FAU - Wang, Zhen AU - Wang Z AD - Special Inspection Department, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Shandong, China; Department of Interventional Ultrasound, the First Medical Center of Chinese PLA General Hospital, Beijing, China. FAU - Liang, Ping AU - Liang P AD - Department of Interventional Ultrasound, the First Medical Center of Chinese PLA General Hospital, Beijing, China. Electronic address: liangping301@126.com. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Ultrasound Med Biol JT - Ultrasound in medicine & biology JID - 0410553 SB - IM OTO - NOTNLM OT - Hepatocellular carcinoma OT - Microwave ablation OT - Modified Charlson Comorbidity Index OT - Overlap weighting OT - The elderly COIS- Conflict of interest The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/29 23:02 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/06/04 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/29 23:02 [entrez] AID - S0301-5629(26)00400-X [pii] AID - 10.1016/j.ultrasmedbio.2026.09.024 [doi] PST - aheadofprint SO - Ultrasound Med Biol. 2026 Oct 29:S0301-5629(26)00400-X. doi: 10.1016/j.ultrasmedbio.2026.09.024. PMID- 42957515 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-291X (Electronic) IS - 0301-5629 (Linking) DP - 2026 Oct 29 TI - Ultrasound Localization Microscopy as a Novel Imaging Technique for Differentiating Autoimmune Pancreatitis From Pancreatic Ductal Adenocarcinoma: A Preliminary Prospective Study. LID - S0301-5629(26)00395-9 [pii] LID - 10.1016/j.ultrasmedbio.2026.09.021 [doi] AB - OBJECTIVE: Focal autoimmune pancreatitis (AIP) mimics pancreatic ductal adenocarcinoma (PDAC) radiologically, posing diagnostic challenges. This exploratory study investigated ultrasound localization microscopy (ULM) for quantitative microvascular characterization and compared its diagnostic performance with that of conventional contrast-enhanced ultrasound (CEUS). METHODS: In this prospective exploratory study, 30 patients with solid pancreatic lesions, including 15 with AIP and 15 with PDAC, underwent conventional ultrasound and CEUS followed by offline ULM reconstruction from arterial-phase raw RF data. Qualitative CEUS features and quantitative time-intensity curve parameters were assessed. Quantitative ULM parameters were extracted from lesion and matched perilesional pancreatic ROIs, and derived heterogeneity metrics were calculated. Histological vascular area fraction was quantified for correlation analysis. The diagnostic performances of CEUS and ULM parameters were evaluated and compared using receiver operating characteristic analysis with bootstrap-derived 95% confidence intervals. RESULTS: ULM showed significantly greater vascular density and more homogeneous intralesional vascular distribution in AIP than in PDAC (both p < 0.001). AIP also exhibited higher vessel ratio, perfusion index, fractal number, mean velocity, and mean diameter. Among the ULM parameters, the Delta fractal number and normalized fractal number showed the highest diagnostic performance, both achieving an AUC of 0.985. By comparison, the best-performing individual CEUS feature, enhancement intensity, yielded an AUC of 0.800, while its combination with washout pattern increased the AUC to 0.867. Among the quantitative CEUS parameters, half-intensity time and mean transit time performed best with AUCs of 0.880 and 0.864, respectively. ULM vessel ratio showed a significant positive correlation with histological vascular area fraction. CONCLUSION: As a preliminary exploration, ULM demonstrated feasibility for characterizing microvascular differences and yielded higher diagnostic accuracy than CEUS parameters in a selected cohort. These findings require validation in larger, multicenter studies before ULM can be considered as a clinical biomarker for this differential diagnosis. CI - Copyright (c) 2026 World Federation for Ultrasound in Medicine and Biology. Published by Elsevier Inc. All rights reserved. FAU - Liang, Tianyi AU - Liang T AD - Department of Ultrasound, First Medical Center, Chinese PLA General Hospital, Beijing, China. FAU - Ao, Yanyun AU - Ao Y AD - Department of Pathology, First Medical Center, Chinese PLA General Hospital, Beijing, China. FAU - Sun, Hao AU - Sun H AD - Department of Ultrasound, First Medical Center, Chinese PLA General Hospital, Beijing, China. FAU - Zhang, Chao AU - Zhang C AD - Department of Ultrasound, First Medical Center, Chinese PLA General Hospital, Beijing, China. FAU - Wang, Yan AU - Wang Y AD - Department of Ultrasound, First Medical Center, Chinese PLA General Hospital, Beijing, China. FAU - Zhang, Mingbo AU - Zhang M AD - Department of Ultrasound, First Medical Center, Chinese PLA General Hospital, Beijing, China. Electronic address: owsifanduizhe@126.com. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Ultrasound Med Biol JT - Ultrasound in medicine & biology JID - 0410553 SB - IM OTO - NOTNLM OT - Autoimmune pancreatitis OT - Differential diagnosis OT - Microvascular imaging OT - Pancreatic ductal adenocarcinoma OT - Ultrasound localization microscopy COIS- Conflict of interest The authors declare no competing interests. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/29 23:02 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/03 00:00 [received] PHST- 2026/08/29 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 23:02 [entrez] AID - S0301-5629(26)00395-9 [pii] AID - 10.1016/j.ultrasmedbio.2026.09.021 [doi] PST - aheadofprint SO - Ultrasound Med Biol. 2026 Oct 29:S0301-5629(26)00395-9. doi: 10.1016/j.ultrasmedbio.2026.09.021. PMID- 42957514 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-291X (Electronic) IS - 0301-5629 (Linking) DP - 2026 Oct 29 TI - Endorectal Ultrasound-Based Habitat Analysis for Pathologic Response Prediction After Neoadjuvant Chemotherapy in Locally Advanced Rectal Cancer. LID - S0301-5629(26)00398-4 [pii] LID - 10.1016/j.ultrasmedbio.2026.09.022 [doi] AB - OBJECTIVE: To investigate the value of endorectal ultrasound (ERUS)-based habitat analysis in predicting pathologic response to neoadjuvant chemotherapy (NCT) in locally advanced rectal cancer (LARC). METHODS: Consecutive patients treated between January 2018 and May 2025 were randomly divided into training and test sets at a 7:3 ratio. Tumor habitat subregions were defined using K-means clustering, and radiomic features were extracted and selected. Clinical, habitat, and fusion models were developed, and model performance was evaluated using ROC analysis, calibration curves, and decision curve analysis (DCA). RESULTS: Among 407 patients, tumor location, cT stage, extramural vascular invasion, and tumor-stroma ratio differed significantly between good- and poor-response groups (p < 0.05). For predicting response to NCT, the clinical, habitat, and fusion models achieved AUCs of 0.885 (95% CI: 0.840-0.927), 0.911 (95% CI: 0.872-0.943), and 0.958 (95% CI: 0.935-0.979) in the training set and 0.757 (95% CI: 0.668-0.841), 0.720 (95% CI: 0.599-0.828), and 0.829 (95% CI: 0.737-0.906) in the test set, respectively. In the test set, the fusion model outperformed the habitat model (p = 0.010), whereas the difference from the clinical model was not statistically significant (p = 0.097). The fusion model exhibited the highest sensitivity in both sets (0.956 and 0.897, respectively). In the test set, it yielded the lowest Brier score (0.174) and generally provided the greatest net benefit across most threshold probabilities of 0.12 to 0.75. CONCLUSION: The fusion model demonstrated promising performance for predicting response to NCT in LARC. Prospective multicenter external validation is required before clinical implementation. CI - Copyright (c) 2026 World Federation for Ultrasound in Medicine and Biology. Published by Elsevier Inc. All rights reserved. FAU - Yi, Hang AU - Yi H AD - Department of Medical Ultrasonics, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China; Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China. FAU - Chen, Limei AU - Chen L AD - Department of Medical Ultrasonics, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China; Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China. FAU - Liu, Xiaoyin AU - Liu X AD - Department of Medical Ultrasonics, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China; Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China. FAU - Chen, Junzhao AU - Chen J AD - Department of Medical Ultrasonics, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China; Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China. FAU - Su, Mengxue AU - Su M AD - Department of Medical Ultrasonics, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China; Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China. FAU - Liu, Guangjian AU - Liu G AD - Department of Medical Ultrasonics, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China; Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China; Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China. Electronic address: liugj@mail.sysu.edu.cn. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Ultrasound Med Biol JT - Ultrasound in medicine & biology JID - 0410553 SB - IM OTO - NOTNLM OT - Endorectal ultrasound OT - Habitat radiomics OT - Locally advanced rectal cancer OT - Neoadjuvant chemotherapy COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this article. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/29 23:02 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/05/29 00:00 [received] PHST- 2026/08/24 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/29 23:02 [entrez] AID - S0301-5629(26)00398-4 [pii] AID - 10.1016/j.ultrasmedbio.2026.09.022 [doi] PST - aheadofprint SO - Ultrasound Med Biol. 2026 Oct 29:S0301-5629(26)00398-4. doi: 10.1016/j.ultrasmedbio.2026.09.022. PMID- 42957503 OWN - NLM STAT- Publisher LR - 20261029 IS - 1532-8686 (Electronic) IS - 0037-1963 (Linking) DP - 2026 Oct 3 TI - Outcomes and challenges of stem cell transplantation in patients with ribosomopathies. LID - S0037-1963(26)00097-1 [pii] LID - 10.1053/j.seminhematol.2026.09.009 [doi] AB - Ribosomopathies are a heterogeneous group of rare inherited disorders caused by defects in ribosome biogenesis or function, leading to bone marrow failure (BMF), congenital anomalies, immune dysfunction, and increased risk of malignancy. Ribosomopathies associated with hematological phenotype include Shwachman-Diamond syndrome (SDS), Diamond-Blackfan anemia (DBA), and cartilage-hair hypoplasia (CHH). Allogenic hematopoietic cell transplantation (HCT) remains the only curative treatment for the hematologic manifestations of these conditions, although it does not correct their extra-hematologic features. In SDS, HCT is indicated for progressive bone marrow failure, myelodysplastic syndrome (MDS), acute myeloid leukemia (AML), or high-risk clonal evolution. Recent insights into TP53-driven leukemogenesis have improved risk stratification and may support earlier transplantation. Nevertheless, role of TP53 monitoring, timing of HCT, cytoreduction before HCT in MDS/AML, and poor outcomes after leukemic transformation remain major challenges. In DBA, HCT is reserved for transfusion dependence, steroid refractoriness/dependence, BMF, immunodeficiency, or clonal evolution. Outcomes have improved substantially, with recent studies demonstrating comparable survival after matched sibling donor (MSD) and matched unrelated donor (MUD) transplantation. Careful management of iron overload and lifelong cancer surveillance are essential components of care. In CHH, HCT is indicated for severe immunodeficiency, transfusion-dependent anemia, and BMF. Across all ribosomopathies, outcomes are most favorable when transplantation is performed before irreversible organ damage or malignant transformation. Advances in molecular monitoring, particularly for clonal evolution, together with emerging gene therapy and gene-editing approaches, are opening new therapeutic perspectives and may eventually provide alternatives to HCT for selected patients. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Pierri, Filomena AU - Pierri F AD - HSCT Unit, Department of Hemato-Oncology, IRCCS Istituto G. Gaslini, Genova, Italy. FAU - Dufour, Carlo AU - Dufour C AD - Hematology Unit, Department of Hemato-Oncology, IRCCS Istituto G. Gaslini, Genova, Italy. Electronic address: carlodufour@gaslini.org. LA - eng PT - Journal Article DEP - 20261003 PL - United States TA - Semin Hematol JT - Seminars in hematology JID - 0404514 SB - IM OTO - NOTNLM OT - Allogeneic hematopoietic cell transplantation OT - Cartilage-hair hypoplasia OT - Diamond-Blackfan anemia syndrome OT - Ribosomopathies OT - Shwachman-Diamond syndrome COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/29 23:01 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/06/16 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/29 23:01 [entrez] AID - S0037-1963(26)00097-1 [pii] AID - 10.1053/j.seminhematol.2026.09.009 [doi] PST - aheadofprint SO - Semin Hematol. 2026 Oct 3:S0037-1963(26)00097-1. doi: 10.1053/j.seminhematol.2026.09.009. PMID- 42957502 OWN - NLM STAT- Publisher LR - 20261029 IS - 1532-1770 (Electronic) IS - 1521-6942 (Linking) DP - 2026 Oct 29 TI - The role of platelet-rich plasma and stem cells in the management of osteoarthritis: Current evidence, clinical positioning, and translational challenges. PG - 102213 LID - S1521-6942(26)00098-7 [pii] LID - 10.1016/j.berh.2026.102213 [doi] AB - Platelet-rich plasma (PRP) and mesenchymal stromal cell (MSC) therapies are among the most widely used regenerative interventions for knee osteoarthritis (OA). Despite being driven by a broadly similar regenerative concept, important differences in biological characteristics, evidence availability, and regulatory requirements mean that they should not be regarded as broadly comparable therapies. At the same time, conflicting findings from clinical trials and evidence syntheses continue to shape interpretation and clinical decision-making. Substantial heterogeneity across products, treatment protocols, patient populations, and study designs has further complicated the evidence base. Yet as public enthusiasm and commercialization continue to accelerate, these uncertainties are often overshadowed by simplified narratives and therapeutic expectations. Amid this uncertainty, clinical interpretation should prioritise reliable evidence over expectation-driven narratives. In this rapidly evolving landscape, regenerative therapies should be considered through a cautious, evidence-based, and guideline-informed lens. Current evidence does not support routine, indiscriminate, or first-line use of regenerative injections in knee OA. PRP may be discussed as a selective option for carefully selected patients after inadequate response to established conservative treatments, but uncertainty about the magnitude and durability of benefit, together with caveats related to reporting limitations, spin, and costs, should be clearly communicated to support shared decision-making. MSC-based and other cell-based therapies remain less established and require further rigorous evaluation before broader clinical adoption. CI - Copyright (c) 2026 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Fan, DingYuan AU - Fan D AD - Sydney Musculoskeletal Health, The Kolling Institute, The University of Sydney, Sydney, New South Wales, Australia. Electronic address: dfan0814@uni.sydney.edu.au. FAU - Hunter, David AU - Hunter D AD - Sydney Musculoskeletal Health, The Kolling Institute, The University of Sydney, Sydney, New South Wales, Australia; Department of Rheumatology, Royal North Shore Hospital and Northern Clinical School, The University of Sydney, Sydney, New South Wales, 2065, Australia. Electronic address: david.hunter@sydney.edu.au. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - Netherlands TA - Best Pract Res Clin Rheumatol JT - Best practice & research. Clinical rheumatology JID - 101121149 SB - IM COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/30 05:30 MHDA- 2026/10/30 05:30 CRDT- 2026/10/29 23:01 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/30 05:30 [pubmed] PHST- 2026/07/08 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/29 23:01 [entrez] AID - S1521-6942(26)00098-7 [pii] AID - 10.1016/j.berh.2026.102213 [doi] PST - aheadofprint SO - Best Pract Res Clin Rheumatol. 2026 Oct 29:102213. doi: 10.1016/j.berh.2026.102213. PMID- 42957497 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-2623 (Electronic) IS - 0041-1345 (Linking) DP - 2026 Oct 29 TI - Pre-Implantation Kidney Biopsy: Assessing Safety in Transplant Outcomes. LID - S0041-1345(26)00520-8 [pii] LID - 10.1016/j.transproceed.2026.09.026 [doi] AB - BACKGROUND: Pre-implantation kidney biopsy is commonly used to guide deceased donor organ allocation, despite ongoing controversy regarding its predictive value and potential impact on recipient outcomes. Evidence addressing the safety of this procedure from the recipient's perspective remains limited. METHODS: We performed a cross-sectional retrospective study including adult recipients of single deceased-donor renal transplant at Coimbra Local Health Unit between January 2020 and December 2022. Recipients were stratified according to whether the graft underwent pre-implantation biopsy (allocation or zero-time) or not. Early postoperative outcomes included transfusion requirements, hemoglobin variation at 48 hours, primary nonfunction, and cold ischemia time. RESULTS: Among 257 recipients, 114 (44.4%) received a biopsied graft. Cold ischemia time was slightly longer in biopsied grafts, but not significantly (17.9 vs 16.6 hours; p = .08). Pre-implantation biopsy was not associated with increased transfusion requirements (chi(2) = 0.475, p = 0.491). In non-transfused recipients, hemoglobin variation at 48 hours was comparable between biopsied and non-biopsied grafts (-21.2% vs -21.1%; p = .990). Rates of primary nonfunction (p = .518) did not differ significantly between groups. Two early postoperative deaths occurred in recipients of biopsied grafts, neither related to the biopsy procedure. CONCLUSIONS: In our cohort, pre-implantation renal graft biopsy was not associated with worse early postoperative outcomes. These findings support the safety of selectively performed procurement biopsies in kidney transplantation practice. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Pimenta, Ana Carolina AU - Pimenta AC AD - Department of Nephrology, Coimbra Local Health Unit, Coimbra, Portugal. Electronic address: accpimenta@gmail.com. FAU - Fernandes, Beatriz Pedro AU - Fernandes BP AD - Department of Nephrology, Coimbra Local Health Unit, Coimbra, Portugal. FAU - Esteves, Alexandra AU - Esteves A AD - Department of Nephrology, Coimbra Local Health Unit, Coimbra, Portugal. FAU - Fragoso, Pedro AU - Fragoso P AD - Department of Nephrology, Coimbra Local Health Unit, Coimbra, Portugal. FAU - Leal, Rita AU - Leal R AD - Department of Nephrology, Coimbra Local Health Unit, Coimbra, Portugal; University Clinic of Nephrology, Faculty of Medicine, University of Coimbra, Portugal. FAU - Guedes Marques, Maria AU - Guedes Marques M AD - Department of Nephrology, Coimbra Local Health Unit, Coimbra, Portugal; University Clinic of Nephrology, Faculty of Medicine, University of Coimbra, Portugal. FAU - Santos, Lidia AU - Santos L AD - Department of Nephrology, Coimbra Local Health Unit, Coimbra, Portugal; University Clinic of Nephrology, Faculty of Medicine, University of Coimbra, Portugal. FAU - Romaozinho, Catarina AU - Romaozinho C AD - Department of Nephrology, Coimbra Local Health Unit, Coimbra, Portugal; University Clinic of Nephrology, Faculty of Medicine, University of Coimbra, Portugal. FAU - Figueiredo, Arnaldo AU - Figueiredo A AD - Department of Urology and Renal Transplantation, Coimbra Local Health Unit, Coimbra, Portugal. FAU - Alves, Rui AU - Alves R AD - Department of Nephrology, Coimbra Local Health Unit, Coimbra, Portugal; University Clinic of Nephrology, Faculty of Medicine, University of Coimbra, Portugal. FAU - Sa, Helena AU - Sa H AD - Department of Nephrology, Coimbra Local Health Unit, Coimbra, Portugal; University Clinic of Nephrology, Faculty of Medicine, University of Coimbra, Portugal. FAU - Rodrigues, Luis AU - Rodrigues L AD - Department of Nephrology, Coimbra Local Health Unit, Coimbra, Portugal; University Clinic of Nephrology, Faculty of Medicine, University of Coimbra, Portugal. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Transplant Proc JT - Transplantation proceedings JID - 0243532 SB - IM COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 23:01 PHST- 2026/02/19 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 23:01 [entrez] AID - S0041-1345(26)00520-8 [pii] AID - 10.1016/j.transproceed.2026.09.026 [doi] PST - aheadofprint SO - Transplant Proc. 2026 Oct 29:S0041-1345(26)00520-8. doi: 10.1016/j.transproceed.2026.09.026. PMID- 42957496 OWN - NLM STAT- Publisher LR - 20261029 IS - 1878-3562 (Electronic) IS - 1590-8658 (Linking) DP - 2026 Oct 29 TI - French prospective cohort study of predictors of fruquintinib efficacy in real-world metastatic colorectal cancer (PRODIGE 108 - COHORTE 2320 - COFRUQ). LID - S1590-8658(26)00989-8 [pii] LID - 10.1016/j.dld.2026.10.010 [doi] AB - BACKGROUND: Colorectal cancer (CRC) is the third most common cancer and the second leading cause of cancer-related deaths worldwide. In unresectable metastatic (m)CRC, systemic therapy is the standard of care, and maintaining anti-angiogenic pressure remains important in the chemorefractory setting, as shown in 2nd and 3rd line treatment. Fruquintinib, a selective tyrosine kinase inhibitor of VEGF receptors 1, 2, and 3, recently demonstrated a 2.6-month overall survival benefit versus placebo in advanced mCRC and is now a new option for heavily pre-treated patients. However, real-world data on fruquintinib use and predictors of its efficacy remain limited. DESIGN: PRODIGE108-COHORTE2320 - COFRUQ (NCT07124858) is a French multicentre prospective observational cohort primarily designed to identify clinical and biological factors associated with fruquintinib efficacy under real-world conditions. The primary endpoint is investigator-assessed overall survival. Main secondary objectives include progression-free survival, objective response rate, disease control rate, safety, and quality of life. Eligible patients are >/=18 years old with histologically confirmed mCRC receiving fruquintinib within its EMA indication. Biological studies (tumour and blood collection) will identify prognostic and predictive biomarkers, alongside pharmacokinetic analyses. The cohort aims to enrol 300 patients over 3 years and has been open for enrolment in France since March 2026. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Muller, Marie AU - Muller M AD - University of Lorraine, Department of Hepato-Gastroenterology and Digestive Oncology, Nancy University Hospital, Nancy, France. Electronic address: m.muller7@chru-nancy.fr. FAU - Aparicio, Thomas AU - Aparicio T AD - Department of Gastroenterology, Universite Paris Cite, Saint Louis Hospital, APHP, Paris, France. FAU - Cohen, Romain AU - Cohen R AD - Sorbonne Universite and Department of Medical Oncology, Saint-Antoine Hospital, Paris, France. FAU - Gallois, Claire AU - Gallois C AD - Department of Gastroenterology and Digestive Oncology, Hopital Europeen Georges Pompidou, Paris, France. FAU - Delattre, Alexis AU - Delattre A AD - Federation Francophone de Cancerologie Digestive, Universite Bourgogne Europe, INSERM CTM 1231 EPICAD, Dijon, France. FAU - Paillaud, Elena AU - Paillaud E AD - Department of Geriatric Medicine, Hopital Europeen Georges Pompidou, Paris, France. FAU - Mazard, Thibault AU - Mazard T AD - Department of Medical oncology, Montpellier Cancer Institute (ICM), Montpellier University, Montpellier, France. FAU - Pelkowski, Manon AU - Pelkowski M AD - Federation Francophone de Cancerologie Digestive, Universite Bourgogne Europe, INSERM CTM 1231 EPICAD, Dijon, France. FAU - Phelip, Jean-Marc AU - Phelip JM AD - University Hospital of Saint Etienne, Saint Etienne, France;, Universite Jean Monnet/Claude Bernard Lyon 1, Unite HESPER EA-7425, France. FAU - Randrian, Violaine AU - Randrian V AD - Department of Hepatology and Gastroenterology, Poitiers University Hospital, Poitiers, France. FAU - Laurent-Puig, Pierre AU - Laurent-Puig P AD - Centre de Recherche des Cordeliers, Sorbonne Universite, Inserm, Universite Paris Cite, Personalized Medicine, Phamacogenomics and Therapeutic Optimization, Institut du Cancer Paris CARPEM, APHP. Centre, Department of Biology, Hopital Europeen Georges Pompidou, Paris, France. FAU - Goldwirt, Lauriane AU - Goldwirt L AD - Department of Tumor Genomics and Pharmacology, Universite Paris Cite, Saint Louis Hospital, APHP, Paris, France. FAU - Perret, Audrey AU - Perret A AD - Department of Medical Oncology, Niort general hospital, Niort, France. FAU - Bachet, Jean-Baptiste AU - Bachet JB AD - Department of Gastroenterology, La Pitie Salpetriere Hospital, APHP, Paris, France. FAU - Akouz, Faiza Khemissa AU - Akouz FK AD - Department of Gastroenterology and Digestive Oncology, Perpignan general Hospital, Perpignan, France. FAU - Couteau, Corinne AU - Couteau C AD - Department of Medical Oncology, Albi general hospital, Albi, France. FAU - Hautefeuille, Vincent AU - Hautefeuille V AD - Department of Gastroenterology and Digestive Oncology, Amiens University Hospital, Amiens, France. FAU - Lepage, Come AU - Lepage C AD - Federation Francophone de Cancerologie Digestive (FFCD), University of Burgundy and Franche Comte, EPICAD INSERM LNC-UMR 1231, Dijon, France. FAU - Tougeron, David AU - Tougeron D AD - Department of Hepatology and Gastroenterology, Poitiers University Hospital, Poitiers, France. LA - eng PT - Journal Article DEP - 20261029 PL - Netherlands TA - Dig Liver Dis JT - Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver JID - 100958385 SB - IM OTO - NOTNLM OT - Fruquintinib OT - Metastatic colorectal cancer OT - Prospective cohort OT - Real world data OT - Systemic therapy COIS- Declaration of competing interest DT reports consultancy, advisory fees, honoraria from Servier, Pierre Fabre, Merck Serono, AMGEN, MSD, BMS, Astra Zeneca, Roche, Gilead, Astellas, BeOne, Takeda, Incyte, Sanofi; research funding from Sandoz, Astra Zeneca, Servier, MSD, Takeda; travel grants from Pierre Fabre, MSD, Servier, Roche. MMu reports consultancy, advisory fees, honoraria from Pierre Fabre, Merck Serono, MSD, Takeda; travel grants from Pierre Fabre, Servier. Other authors: no conflict of interest to declare. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 23:01 PHST- 2026/07/03 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 23:01 [entrez] AID - S1590-8658(26)00989-8 [pii] AID - 10.1016/j.dld.2026.10.010 [doi] PST - aheadofprint SO - Dig Liver Dis. 2026 Oct 29:S1590-8658(26)00989-8. doi: 10.1016/j.dld.2026.10.010. PMID- 42957493 OWN - NLM STAT- Publisher LR - 20261029 IS - 1878-3562 (Electronic) IS - 1590-8658 (Linking) DP - 2026 Oct 29 TI - Somatic mutations in coeliac disease and other enteropathies: From chronic inflammation to clonal immune disease. LID - S1590-8658(26)00977-1 [pii] LID - 10.1016/j.dld.2026.10.001 [doi] AB - Somatic mutations are increasingly implicated in persistent intestinal inflammation in chronic enteropathies. This review examines how mutation-bearing immune and epithelial clones may emerge, expand and contribute to disease within the chronically inflamed gut. We focus on refractory coeliac disease, highlighting the recent evidence for lymphoma-associated mutations in expanded intraepithelial lymphocyte clones in both type I and type II disease. We then consider emerging evidence in other enteropathy, including autoimmune enteropathy, common carriable immunodeficiency-associated enteropathy and inflammatory bowel disease. Finally, we discuss how inflammation shapes clonal selection in the intestine, approaches for detecting low-frequency somatic variants, and the potential implications of mutation profiling for diagnosis, risk stratification and future therapeutic strategies. CI - Copyright (c) 2026 The Author(s). Published by Elsevier B.V. All rights reserved. FAU - Singh, Mandeep AU - Singh M AD - Westmead Institute for Medical Research, Westmead, Sydney, NSW, Australia. FAU - Schumann, Michael AU - Schumann M AD - Dept. for Gastroenterology, Rheumatology and Infectious Diseases, Charite - University Medicine Berlin, Berlin, Germany. FAU - Elli, Luca AU - Elli L AD - Universita' Statale Milano, Department of Pathophysiology and Transplantation Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milano, Italy. FAU - Luciani, Fabio AU - Luciani F AD - Westmead Institute for Medical Research, Westmead, Sydney, NSW, Australia; School of Biomedical Sciences, UNSW Sydney, NSW, Australia. Electronic address: luciani@unsw.edu.au. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - Netherlands TA - Dig Liver Dis JT - Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver JID - 100958385 SB - IM OTO - NOTNLM OT - Autoimmune enteropathy OT - Clonal evolution OT - Clonal haematopoiesis OT - Enteropathies OT - Intestinal T-cell lymphoma OT - JAK-STAT signalling OT - Refractory coeliac disease OT - Somatic mutations COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 23:01 PHST- 2026/05/29 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 23:01 [entrez] AID - S1590-8658(26)00977-1 [pii] AID - 10.1016/j.dld.2026.10.001 [doi] PST - aheadofprint SO - Dig Liver Dis. 2026 Oct 29:S1590-8658(26)00977-1. doi: 10.1016/j.dld.2026.10.001. PMID- 42957490 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-2022 (Electronic) IS - 0019-0578 (Linking) DP - 2026 Oct 26 TI - Improved decoupling for maglev planar actuators with coil-to-coil misalignments: A LM iterative commutation tuning algorithm. LID - S0019-0578(26)00539-2 [pii] LID - 10.1016/j.isatra.2026.10.027 [doi] AB - The maglev planar actuator is a core component of ultra-precision motion stages in various industrial applications. Commutation serves as the critical first step in decoupling; however, its performance is usually limited by the mismatch between the actual and theoretical electromagnetic models. To address this issue, this paper proposes a data-based Levenberg-Marquardt (LM) iterative commutation tuning strategy. Unlike existing methods that consider only mover-to-stator misalignments, the proposed strategy models and tunes the phase errors of individual coils, thereby providing a more accurate commutation model. Moreover, the LM algorithm is employed to solve the resulting nonlinear least-squares problem, providing improved robustness and tuning performance compared with the gradient-descent (GD) algorithm. Experimental results on a moving-coil maglev planar actuator (MCMPA) show that the proposed strategy remains effective with tuning points selected along the X DOF, along the Y DOF, or distributed along both DOFs, and reduces the peak Z-DOF coupling error induced by X/Y-DOF movements by at least 47.7% under high-dynamic tracking. Furthermore, the proposed strategy outperforms the GD-based tuning method and the approach considering only mover-to-stator misalignments, while the tuned parameters can also be successfully applied to the direct decoupling model. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Qin, Guangyi AU - Qin G AD - Center of Ultra-Precision Optoelectronic Instrument Engineering, Harbin Institute of Technology, Harbin 150080, China; Key Lab of Ultra-Precision Intelligent Instrumentation (Harbin Institute of Technology), Ministry of Industry and Information Technology, Harbin 150080, China. Electronic address: 21b901033@stu.hit.edu.cn. FAU - Song, Shuo AU - Song S AD - Center of Ultra-Precision Optoelectronic Instrument Engineering, Harbin Institute of Technology, Harbin 150080, China; Key Lab of Ultra-Precision Intelligent Instrumentation (Harbin Institute of Technology), Ministry of Industry and Information Technology, Harbin 150080, China. Electronic address: 23b901017@stu.hit.edu.cn. FAU - Li, Li AU - Li L AD - Center of Ultra-Precision Optoelectronic Instrument Engineering, Harbin Institute of Technology, Harbin 150080, China; Key Lab of Ultra-Precision Intelligent Instrumentation (Harbin Institute of Technology), Ministry of Industry and Information Technology, Harbin 150080, China. Electronic address: hitlili@hit.edu.cn. FAU - Liu, Yang AU - Liu Y AD - Center of Ultra-Precision Optoelectronic Instrument Engineering, Harbin Institute of Technology, Harbin 150080, China; Key Lab of Ultra-Precision Intelligent Instrumentation (Harbin Institute of Technology), Ministry of Industry and Information Technology, Harbin 150080, China. Electronic address: hitlg@hit.edu.cn. FAU - Zou, Limin AU - Zou L AD - Center of Ultra-Precision Optoelectronic Instrument Engineering, Harbin Institute of Technology, Harbin 150080, China; Key Lab of Ultra-Precision Intelligent Instrumentation (Harbin Institute of Technology), Ministry of Industry and Information Technology, Harbin 150080, China. Electronic address: zoulimin@hit.edu.cn. FAU - Tan, Jiubin AU - Tan J AD - Center of Ultra-Precision Optoelectronic Instrument Engineering, Harbin Institute of Technology, Harbin 150080, China; Key Lab of Ultra-Precision Intelligent Instrumentation (Harbin Institute of Technology), Ministry of Industry and Information Technology, Harbin 150080, China. Electronic address: jbtan@hit.edu.cn. LA - eng PT - Journal Article DEP - 20261026 PL - United States TA - ISA Trans JT - ISA transactions JID - 0374750 SB - IM OTO - NOTNLM OT - Commutation OT - Decoupling OT - Iterative learning OT - Iterative tuning OT - Maglev planar actuator OT - Motion control COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 23:01 PHST- 2026/05/24 00:00 [received] PHST- 2026/10/23 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 23:01 [entrez] AID - S0019-0578(26)00539-2 [pii] AID - 10.1016/j.isatra.2026.10.027 [doi] PST - aheadofprint SO - ISA Trans. 2026 Oct 26:S0019-0578(26)00539-2. doi: 10.1016/j.isatra.2026.10.027. PMID- 42957468 OWN - NLM STAT- Publisher LR - 20261029 IS - 1878-4046 (Electronic) IS - 1076-6332 (Linking) DP - 2026 Oct 29 TI - Development of Entrustable Professional Activities for Core Diagnostic Radiology Procedures: An APDIR Workgroup Initiative. LID - S1076-6332(26)00842-1 [pii] LID - 10.1016/j.acra.2026.10.007 [doi] AB - RATIONALE AND OBJECTIVES: Although entrustable professional activities (EPAs) have been adopted across many medical specialties, their use in radiology remains limited, largely to subspecialty areas such as breast imaging. A standardized set of EPAs for procedural training in diagnostic radiology has not been widely available. We sought to develop EPAs for 10 core diagnostic radiology procedures through a structured, consensus-based process, providing programs with a behaviorally anchored framework for assessing procedural competence. MATERIALS AND METHODS: Using a consensus-based, expert-panel approach with iterative refinement, a volunteer panel of nine integrated interventional radiology (IR) residency program directors from nine distinct academic institutions adapted the American Board of Surgery's general surgery EPA framework as a template. Ten core diagnostic radiology procedures, previously identified by a joint task force of the Association of Program Directors in Radiology and the Association of Program Directors in Interventional Radiology, were selected for development. Subject-matter experts drafted an EPA for each, a single author harmonized them, and the full panel reviewed and approved the final versions. RESULTS: Ten EPAs were developed, one for each of the core diagnostic radiology procedures. Each EPA describes the procedure-specific behaviors expected at each phase of care across four entrustment levels-Limited Participation, Direct Supervision, Indirect Supervision, and Practice-Ready. A completed example is provided in Table 1. CONCLUSION: A shared set of procedural EPAs for diagnostic radiology was developed through a structured, consensus-based process built on an established general surgery framework. These EPAs give programs a practical way to assess procedural competence and to support decisions about the level of supervision a resident requires for a given procedure. CI - Copyright (c) 2026 The Association of University Radiologists. Published by Elsevier Inc. All rights reserved. FAU - Patel, Achintya AU - Patel A AD - Interventional Radiology, Atrium Health Wake Forest Baptist, One Medical Center Blvd, Winston-Salem 27157, North Carolina. Electronic address: Achintya.patel@advocatehealth.org. FAU - Kee-Sampson, Joanna AU - Kee-Sampson J AD - Interventional Radiology, Atrium Health Wake Forest Baptist, One Medical Center Blvd, Winston-Salem 27157, North Carolina. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Acad Radiol JT - Academic radiology JID - 9440159 SB - IM OTO - NOTNLM OT - Entrustable professional activities OT - Radiology COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 23:00 PHST- 2026/07/09 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 23:00 [entrez] AID - S1076-6332(26)00842-1 [pii] AID - 10.1016/j.acra.2026.10.007 [doi] PST - aheadofprint SO - Acad Radiol. 2026 Oct 29:S1076-6332(26)00842-1. doi: 10.1016/j.acra.2026.10.007. PMID- 42957464 OWN - NLM STAT- Publisher LR - 20261029 IS - 1532-9496 (Electronic) IS - 0887-7963 (Linking) DP - 2026 Oct 6 TI - Community-Based Approaches to Diversifying the Blood Donor Base: A Critical Review. PG - 151052 LID - S0887-7963(26)00093-3 [pii] LID - 10.1016/j.tmrv.2026.151052 [doi] AB - This review examines community-based approaches to diversifying the blood donor base, with particular attention to the persistent underrepresentation of ethnic and racial minority groups in blood donation systems across the Global North. Drawing on three influential studies of community-based initiatives, the paper critically evaluates dominant recruitment strategies and their underlying assumptions. While existing approaches often emphasize culturally tailored interventions to address group-specific barriers, this review argues that such strategies remain limited by their focus on individual behaviour and essentializing cultural difference. Through a comparative analysis of the selected cases, the review identifies contrasting organizational models of community engagement, ranging from top-down, institution-led interventions and 'double-targeted' strategies involving both communities and staff, to bottom-up, grassroots initiatives that reposition blood donation through religious meaning and practice. Building on these findings, the paper advances a typology of community-based approaches based on two dimensions: the degree to which communities are positioned peripherally or embedded, and the extent to which initiatives are driven from the top down or emerge from the bottom up. The analysis highlights how different approaches distribute agency, authority, and responsibility between blood collection agencies and communities, and demonstrates the importance of relational dynamics and organizational context in shaping participation. The findings also draw attention to structural constraints within blood collection organisations and suggest more systematic research on features of the blood supply chain. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Hustinx, Lesley AU - Hustinx L AD - Department of Sociology, Ghent University, Gent, Belgium. Electronic address: lesley.hustinx@ugent.be. LA - eng PT - Journal Article PT - Review DEP - 20261006 PL - United States TA - Transfus Med Rev JT - Transfusion medicine reviews JID - 8709027 SB - IM OTO - NOTNLM OT - Blood donation OT - Blood supply chain OT - Community-based approaches OT - Diversity policy OT - Ethnic minority donors COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 23:00 PHST- 2026/06/02 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 23:00 [entrez] AID - S0887-7963(26)00093-3 [pii] AID - 10.1016/j.tmrv.2026.151052 [doi] PST - aheadofprint SO - Transfus Med Rev. 2026 Oct 6:151052. doi: 10.1016/j.tmrv.2026.151052. PMID- 42957460 OWN - NLM STAT- Publisher LR - 20261029 IS - 1468-2060 (Electronic) IS - 0003-4967 (Linking) DP - 2026 Oct 29 TI - Interleukin 4-receptor-alpha blockade in localised scleroderma: a phase 2 proof-of-concept study. LID - S0003-4967(26)00605-9 [pii] LID - 10.1016/j.ard.2026.09.022 [doi] AB - OBJECTIVES: Fibrotic diseases are a major therapeutic challenge. In humans, fibrosing diseases are often associated with an interleukin (IL-) 4/T(H)2 signature. In preclinical mouse models, development of fibrosis critically depends on IL-4/signal transducer and activator of transcription 6 (STAT6). Yet, the role of IL-4/IL-13 in human fibrosing diseases remains unknown, and treatment development requires a deeper understanding of the role of IL-4/IL-13 in the pathogenesis. The local inflammation and the development of fibrosis in localised scleroderma (LS) are similar to those in systemic sclerosis and other fibrosing diseases. METHODS: In this article, we investigated the role of IL-4/IL-13 signalling in LS in a randomised, placebo-controlled clinical trial (ClinicalTrials.gov registration: NCT04200755). We studied the effect of 6 months of blocking IL-4-receptor alpha with dupilumab on disease progression, sclerosis-associated inflammation, and sclerosis-specific gene expression. RESULTS: Already this short-term intervention resulted in a significant clinical improvement over placebo, a significant attenuation of the local inflammation, a specific and significant reduction of the IL-4/IL-13-dependent gene signature, and a partial suppression of genes coding for fibrosis-inducing proteins. CONCLUSIONS: Thus, selective inhibition of IL-4/IL-13 prevents the progression of LS and is promising for therapeutic prevention of other fibrosing diseases. CI - Copyright (c) 2026 The Author(s). Published by Elsevier B.V. All rights reserved. FAU - Eming, Sabine A AU - Eming SA AD - Department of Dermatology, Cluster of Excellence Cellular Stress Responses in Ageing-associated Diseases, Center for Molecular Medicine Cologne, Institute of Zoology, Developmental Biology, University of Cologne, Cologne, Germany. Electronic address: sabine.eming@uni-koeln.de. FAU - Kreuter, Alexander AU - Kreuter A AD - Department of Dermatology, University Witten/Herdecke, Witten, Germany. FAU - Hahn, Matthias AU - Hahn M AD - Department of Dermatology, Eberhard Karls University Tubingen, Tubingen, Germany. FAU - Ghoreschi, Kamran AU - Ghoreschi K AD - Department of Dermatology, Charite, Universitatsmedizin Berlin, Berlin, Germany. FAU - Moinzadeh, Pia AU - Moinzadeh P AD - Department of Dermatology, University of Cologne, Cologne, Germany. FAU - Hunzelmann, Nicolas AU - Hunzelmann N AD - Department of Dermatology, University of Cologne, Cologne, Germany. FAU - Becker, Kerstin AU - Becker K AD - Cologne Center for Genomics, Medical Faculty, University of Cologne, Cologne, Germany. FAU - Reiche, Nils AU - Reiche N AD - Department of Dermatology, University of Cologne, Cologne, Germany. FAU - Adams, Anne AU - Adams A AD - Institute of Medical Statistics and Computational Biology, University of Cologne, Cologne, Germany. FAU - Shah-Hosseini, Kija AU - Shah-Hosseini K AD - Institute of Medical Statistics and Computational Biology, University of Cologne, Cologne, Germany. FAU - Hellmich, Martin AU - Hellmich M AD - Institute of Medical Statistics and Computational Biology, University of Cologne, Cologne, Germany. FAU - Willenborg, Sebastian AU - Willenborg S AD - Department of Dermatology, University of Cologne, Cologne, Germany. FAU - Sanin, David E AU - Sanin DE AD - Bloomberg-Kimmel Institute for Cancer Immunotherapy, Johns Hopkins School of Medicine, Baltimore, MD, USA. FAU - Rocken, Martin AU - Rocken M AD - Department of Dermatology, Eberhard Karls University Tubingen, Tubingen, Germany. FAU - Krieg, Thomas AU - Krieg T AD - Translational Matrix Biology, Department of Dermatology, Cluster of Excellence Cellular Stress Responses in Ageing-associated Diseases, Center for Molecular Medicine Cologne, University of Cologne, Cologne, Germany. LA - eng SI - ClinicalTrials.gov/NCT04200755 PT - Journal Article DEP - 20261029 PL - United States TA - Ann Rheum Dis JT - Annals of the rheumatic diseases JID - 0372355 SB - IM COIS- Competing interests All authors declare they have no competing interests. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 23:00 PHST- 2026/08/13 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 23:00 [entrez] AID - S0003-4967(26)00605-9 [pii] AID - 10.1016/j.ard.2026.09.022 [doi] PST - aheadofprint SO - Ann Rheum Dis. 2026 Oct 29:S0003-4967(26)00605-9. doi: 10.1016/j.ard.2026.09.022. PMID- 42957443 OWN - NLM STAT- In-Process LR - 20261029 IS - 1873-734X (Electronic) IS - 1010-7940 (Linking) VI - 68 IP - 10 DP - 2026 Oct 7 TI - Corrigendum to: Building the Future: A Proposed Framework for Training Residents in Minimally Invasive Cardiac Surgery. LID - ezag243 [pii] LID - 10.1093/ejcts/ezag243 [doi] LA - eng PT - Published Erratum PL - Germany TA - Eur J Cardiothorac Surg JT - European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery JID - 8804069 SB - IM EFR - Eur J Cardiothorac Surg. 2026 Aug 3;68(8):ezag155. doi: 10.1093/ejcts/ezag155. PMID: 42572022 EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 22:52 PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 22:52 [entrez] AID - 9004909 [pii] AID - 10.1093/ejcts/ezag243 [doi] PST - ppublish SO - Eur J Cardiothorac Surg. 2026 Oct 7;68(10):ezag243. doi: 10.1093/ejcts/ezag243. PMID- 42957441 OWN - NLM STAT- Publisher LR - 20261029 IS - 1463-1326 (Electronic) IS - 1462-8902 (Linking) DP - 2026 Oct 29 TI - Daytime Sleepiness, Insomnia Symptoms and Sleep Quality in Adults Entering a Structured Weight-Loss Programme: The SCOOP-RNPC Cohort. LID - 10.1111/dom.71491 [doi] AB - BACKGROUND: Daytime sleepiness, insomnia and poor sleep quality are common in adults with overweight/obesity. This study characterized these sleep-related complaints and their associations with clinical characteristics at entry into the Nutritional and Psycho-Behavioural Rehabilitation (RNPC) programme, identified clinical characteristics associated with impaired sleep health, and explored changes after weight loss. METHODS: This observational study was conducted within the prospective SCOOP-RNPC cohort. Adults with overweight/obesity completed the Epworth Sleepiness Scale (ESS), Insomnia Severity Index (ISI) and Pittsburgh Sleep Quality Index (PSQI) questionnaires. An exploratory questionnaire-based summary score (0-6) was used to describe combined sleep-related profiles and compare baseline clinical characteristics. Longitudinal changes were explored in participants with assessments at baseline and programme end. RESULTS: Of 1886 participants with complete baseline sleep questionnaire data, 20.2% reported excessive daytime sleepiness (ESS score > 10), 23.4% had moderate-to-severe insomnia symptoms (ISI >/= 15) and 63.3% reported poor sleep quality (PSQI > 5). For the exploratory summary score, 22.7%, 50.3% and 27.0% of participants had scores of 0-1, 2-4 and 5-6, respectively. In those with longitudinal data (n = 310), changes in sleep-related questionnaire scores were observed during follow-up, and the proportion with a summary score of 5-6 increased from 31.0% to 45.5%. CONCLUSION: Sleep-related issues were common among adults entering structured weight-loss care. Less favourable questionnaire-based sleep profiles were associated with a greater burden of subjective symptoms. In exploratory longitudinal analyses, improvements in sleep-related questionnaire outcomes were observed during the weight-loss phase, although interpretation is limited by selective follow-up and the absence of a control group. CI - (c) 2026 The Author(s). Diabetes, Obesity and Metabolism published by John Wiley & Sons Ltd. FAU - Fabre, Odile AU - Fabre O AUID- ORCID: 0000-0003-0892-8412 AD - Groupe Ethique et Sante, Aubagne, France. FAU - Bailly, Sebastien AU - Bailly S AUID- ORCID: 0000-0002-2179-4650 AD - HP2 Laboratory, Inserm U1300, Grenoble Alpes University, Grenoble, France. FAU - Coiffier, Ophelie AU - Coiffier O AD - HP2 Laboratory, Inserm U1300, Grenoble Alpes University, Grenoble, France. FAU - Pantagis, Laurent AU - Pantagis L AD - Groupe Ethique et Sante, Aubagne, France. FAU - Legrand, Remy AU - Legrand R AD - Groupe Ethique et Sante, Aubagne, France. FAU - Pepin, Jean-Louis AU - Pepin JL AD - HP2 Laboratory, Inserm U1300, Grenoble Alpes University, Grenoble, France. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Diabetes Obes Metab JT - Diabetes, obesity & metabolism JID - 100883645 SB - IM OTO - NOTNLM OT - obesity OT - overweight OT - sleep OT - sleep quality OT - weight reduction programmes EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 22:43 PHST- 2026/10/14 00:00 [revised] PHST- 2026/08/11 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 22:43 [entrez] AID - 10.1111/dom.71491 [doi] PST - aheadofprint SO - Diabetes Obes Metab. 2026 Oct 29. doi: 10.1111/dom.71491. PMID- 42957435 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2059-2310 (Electronic) IS - 2059-2302 (Linking) VI - 108 IP - 5 DP - 2026 Nov TI - Related and Unrelated Donor Match Presence for Haematopoietic Stem Cell Transplantation in a Portuguese HLA Referral Cohort. LID - e71055 [pii] LID - 10.1111/tan.71055 [doi] AB - Identifying a suitable HLA-matched donor is essential for allogeneic haematopoietic stem cell transplantation (HSCT), but match presence depends on donor source, family workup, matching thresholds, and donor-dataset representation. We retrospectively analysed fully anonymised records from a Portuguese referral cohort (2021-2026), comprising 924 patients with complete six-locus HLA data, evaluated relatives, and Portuguese Bone Marrow Donor Registry records. HLA-A, -B, -C, -DRB1, -DQB1 and -DPB1 assignments were harmonised at high resolution, retaining P groups without conversion to specific two-field alleles. Related donor match presence was evaluated at 12/12, >/= 11/12, >/= 10/12 and probable haploidentical levels, defined by allele sharing at all six loci without confirmed haplotypic phase. Unrelated donor thresholds were 8/8, >/= 7/8, 10/10, >/= 9/10, 12/12 and >/= 11/12. Population-background descriptors, HLA frequency patterns, and exploratory prediction models were also examined. Among all patients, 185 (20.0%) had a 12/12 related donor and 596 (64.5%) a probable haploidentical relative; among 653 patients with an evaluated relative, these proportions were 28.3% and 91.3%. Unrelated donor match presence was 13.1% at 8/8, 48.5% at >/= 7/8, 12.8% at 10/10, 36.5% at >/= 9/10, 2.9% at 12/12 and 12.4% at >/= 11/12. In a same-pool analysis of 23,004 HLA-DPB1-complete donors, match presence decreased from 10.5% (10/10) to 2.9% (12/12), and from 31.8% (>/= 9/10) to 12.4% (>/= 11/12). Strict 12/12 identity was descriptive, not a clinical donor-selection target. Donor match potential is best characterised through structured search profiles integrating family workup, HLA thresholds, HLA-DPB1, and dataset representation. These findings describe genetic match presence within analysed datasets, rather than clinical donor availability. CI - (c) 2026 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. FAU - Ramalhete, Luis AU - Ramalhete L AUID- ORCID: 0000-0002-8911-3380 AD - Blood and Transplantation Center of Lisbon, Instituto Portugues do Sangue e da Transplantacao, Lisboa, Portugal. AD - NOVA Medical School, Faculdade de Ciencias Medicas, Universidade NOVA de Lisboa, Lisbon, Portugal. AD - iNOVA4Health-Advancing Precision Medicine, Nucleo de Investigacao em Doencas Renais, NOVA Medical School, Faculdade de Ciencias Medicas, Universidade NOVA de Lisboa, Lisbon, Portugal. AD - LBS-Lisbon Business & Government School, Lisbon, Portugal. FAU - Almeida, Paula AU - Almeida P AD - Blood and Transplantation Center of Lisbon, Instituto Portugues do Sangue e da Transplantacao, Lisboa, Portugal. FAU - Abade, Olga AU - Abade O AD - Blood and Transplantation Center of Lisbon, Instituto Portugues do Sangue e da Transplantacao, Lisboa, Portugal. FAU - Calao, Sara AU - Calao S AD - Blood and Transplantation Center of Lisbon, Instituto Portugues do Sangue e da Transplantacao, Lisboa, Portugal. FAU - Ferreira, Raquel AU - Ferreira R AD - Blood and Transplantation Center of Lisbon, Instituto Portugues do Sangue e da Transplantacao, Lisboa, Portugal. FAU - Espada, Eduardo AU - Espada E AUID- ORCID: 0000-0002-5713-3440 AD - Blood and Transplantation Center of Lisbon, Instituto Portugues do Sangue e da Transplantacao, Lisboa, Portugal. AD - Servico de Hematologia e Transplantacao de Medula, ULS Santa Maria, Hospital de Santa Maria, Lisboa, Portugal. AD - Faculdade de Medicina da Universidade de Lisboa, Lisboa, Portugal. LA - eng PT - Journal Article PL - England TA - HLA JT - HLA JID - 101675570 RN - 0 (HLA Antigens) SB - IM MH - Humans MH - *Hematopoietic Stem Cell Transplantation MH - Portugal MH - *Histocompatibility Testing MH - *Unrelated Donors MH - Female MH - Male MH - Retrospective Studies MH - Adult MH - Alleles MH - *HLA Antigens/genetics/immunology MH - Registries MH - Middle Aged MH - Adolescent MH - Child MH - Gene Frequency MH - Child, Preschool MH - Infant MH - Young Adult MH - Tissue Donors MH - Haplotypes OTO - NOTNLM OT - HLA matching OT - HLA-DPB1 OT - Portugal OT - donor registry OT - donor search OT - haematopoietic stem cell transplantation OT - haploidentical donor OT - matched related donor OT - population background OT - unrelated donor EDAT- 2026/10/29 23:28 MHDA- 2026/10/30 05:30 CRDT- 2026/10/29 22:33 PHST- 2026/09/15 00:00 [revised] PHST- 2026/07/26 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 22:33 [entrez] AID - e71055 [pii] AID - 10.1111/tan.71055 [doi] PST - ppublish SO - HLA. 2026 Nov;108(5):e71055. doi: 10.1111/tan.71055. PMID- 42957434 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1226-4512 (Print) IS - 1226-4512 (Linking) VI - 30 IP - 6 DP - 2026 Nov 1 TI - PAK4 as a cardiometabolic node: tissue-specific metabolic control, cardiovascular remodeling, and therapeutic trade-offs. PG - 543-561 LID - 10.4196/kjpp.26.314 [doi] AB - Cardiometabolic disease reflects interdependent dysfunction across adipose tissue, liver, skeletal muscle, vasculature, and heart. p21-activated kinase 4 (PAK4), a group II serine/threonine kinase controlling cytoskeletal organization and development, has emerged as a nutrient-responsive regulator in metabolic tissues. We position PAK4 as a candidate cardiometabolic node rather than a proven master regulator or direct energy sensor. Established mechanisms include suppression of adipocyte lipolysis and browning, hepatocyte NCoR1-PPARalpha-dependent fattyacid oxidation and ketogenesis, skeletal-muscle AMPKalpha2-GLUT4 signaling, together with promotion of hepatic stellate-cell activation via YAP phosphorylation at Thr428. These programs could alter the substrate, inflammatory, redox, and endocrine environment encountered by the cardiovascular system. We refine the hepatic ketone deficiency concept into a PAK4-ketone supply-demand mismatch hypothesis: reduced hepatic ketogenesis may limit adaptive ketone availability when myocardial demand rises, though inter-organ causality remains unproven. PAK4 is essential for embryonic cardiac morphogenesis, and limited adult endothelial studies suggest stress-protective roles in ischemic and inflammatory settings, whereas functions in adult cardiomyocytes, fibroblasts, and vascular smooth muscle cells remain largely undefined. An exploratory scan of Gene Expression Omnibus datasets identified recurrent nominal increases in PAK4 mRNA in selected failing or remodeled human myocardium and steatotic liver, but no universal cardiometabolic signature. These findings define a tissue-dependent therapeutic dilemma in which peripheral metabolic benefits of PAK4 inhibition or degradation may coexist with uncharacterized cardiac, isoform, and exposure-related risks. Resolving this paradox will require adult inducible cell-specific models, inter-organ flux studies, cardiac proteomics and phosphoproteomics, and comparison of catalytic inhibitors, degraders, and tissuedirected delivery systems. FAU - Nguyen, To Hoai Thi AU - Nguyen THT AD - Department of Biomedical Sciences, Department of Physiology, College of Medicine, Smart Marine Therapeutic Center, Cardiovascular and Metabolic Disease Center, Inje University, Busan 47392, Korea. FAU - Tran, Khanh Duc AU - Tran KD AD - Department of Biomedical Sciences, Department of Physiology, College of Medicine, Smart Marine Therapeutic Center, Cardiovascular and Metabolic Disease Center, Inje University, Busan 47392, Korea. FAU - Pham, Trong Kha AU - Pham TK AD - Department of Biomedical Sciences, Department of Physiology, College of Medicine, Smart Marine Therapeutic Center, Cardiovascular and Metabolic Disease Center, Inje University, Busan 47392, Korea. AD - VNU University of Science, Vietnam National University, Hanoi 100000, Vietnam. FAU - Seol, Ju Eun AU - Seol JE AD - Department of Biomedical Sciences, Department of Physiology, College of Medicine, Smart Marine Therapeutic Center, Cardiovascular and Metabolic Disease Center, Inje University, Busan 47392, Korea. FAU - Shimizu, Ippei AU - Shimizu I AD - Department of Cardiovascular Aging, National Cerebral and Cardiovascular Center Research Institute, Osaka 564-8565, Japan. FAU - Kim, Hyoung Kyu AU - Kim HK AD - Department of Biomedical Sciences, Department of Physiology, College of Medicine, Smart Marine Therapeutic Center, Cardiovascular and Metabolic Disease Center, Inje University, Busan 47392, Korea. FAU - Han, Jin AU - Han J AUID- ORCID: 0000-0003-1859-3425 AD - Department of Biomedical Sciences, Department of Physiology, College of Medicine, Smart Marine Therapeutic Center, Cardiovascular and Metabolic Disease Center, Inje University, Busan 47392, Korea. LA - eng PT - Journal Article PT - Review PL - Korea (South) TA - Korean J Physiol Pharmacol JT - The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology JID - 9709505 OTO - NOTNLM OT - Cardiovascular diseases OT - Energy metabolism OT - Ketone bodies OT - p21-activated kinase 4 EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 22:33 PHST- 2026/08/30 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 22:33 [entrez] AID - kjpp.26.314 [pii] AID - 10.4196/kjpp.26.314 [doi] PST - ppublish SO - Korean J Physiol Pharmacol. 2026 Nov 1;30(6):543-561. doi: 10.4196/kjpp.26.314. PMID- 42957427 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1942-0870 (Electronic) IS - 1942-0862 (Linking) VI - 18 IP - 1 DP - 2026 Dec 31 TI - SprintX: mitigating data scarcity in antibody design via synthetic structural prior knowledge dataset. PG - 2753234 LID - 10.1080/19420862.2026.2753234 [doi] AB - Structure-based artificial intelligence (AI)-driven antibody design is increasingly important for therapeutic development, yet progress has been hindered by the scarcity of high-quality antibody-antigen complex structures for model training. To alleviate this bottleneck, we introduce SprintX (Structural Prior for Antibody-Antigen CompleXes), a large-scale AlphaFold3-derived synthetic dataset designed to provide structural prior knowledge for antibody-antigen modeling. Unlike conventional docking-based synthesis pipelines, SprintX uses AlphaFold3 to generate antigen-conditioned candidate complex-structure predictions from sampled antibody sequences paired with selected antigens, without imposing predefined binding-site or pose constraints. Candidate structures were ranked to select one representative structure per antibody-antigen pair before terminal trimming and multistage quality control. To improve reliability and learning stability, we applied a multi-stage quality-control pipeline integrating global confidence metrics, stereochemical validation, and interface-level contact analysis. SprintX recapitulated key geometric characteristics and physicochemical regularities of experimentally resolved antibody-antigen complexes while filtering out structurally unstable or implausible synthetic outputs. Across downstream tasks including antibody generation, computational binding-affinity prediction, and binding-interface prediction, models pretrained with SprintX showed meaningful improvements in structural plausibility and competitive performance on computational binding-affinity and binding-interface prediction benchmarks. These results support SprintX not as a substitute for experimentally resolved complexes, but as a complementary source of structural prior knowledge for structure-aware antibody modeling. Finally, we release SprintX as an openly accessible resource to facilitate data-efficient learning for AI-driven antibody design. FAU - Heo, Ryong AU - Heo R AUID- ORCID: 0009-0008-8891-9068 AD - Department of Data Science, Kangwon National University, Chuncheon, Republic of Korea. AD - UBLBio Corporation, Suwon, Republic of Korea. FAU - Lee, Dahyeon AU - Lee D AUID- ORCID: 0009-0002-1041-7232 AD - Department of Data Science, Kangwon National University, Chuncheon, Republic of Korea. FAU - Jung, Jaeho AU - Jung J AUID- ORCID: 0009-0006-6539-7979 AD - Department of Data Science, Kangwon National University, Chuncheon, Republic of Korea. FAU - Kim, Byung Ju AU - Kim BJ AD - UBLBio Corporation, Suwon, Republic of Korea. FAU - Park, Chihyun AU - Park C AUID- ORCID: 0000-0003-4995-2312 AD - Department of Data Science, Kangwon National University, Chuncheon, Republic of Korea. AD - Department of Computer Science and Engineering, Kangwon National University, Chuncheon, Republic of Korea. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - MAbs JT - mAbs JID - 101479829 RN - 0 (Antigen-Antibody Complex) RN - 0 (Antibodies) SB - IM MH - Humans MH - Artificial Intelligence MH - *Antigen-Antibody Complex/chemistry/immunology MH - *Antibodies/chemistry MH - *Drug Design OTO - NOTNLM OT - Synthetic structural dataset OT - antibody-antigen complexes OT - dataset for structure-based generative models OT - interface annotations OT - structural prior data EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 22:13 PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 22:13 [entrez] AID - 10.1080/19420862.2026.2753234 [doi] PST - ppublish SO - MAbs. 2026 Dec 31;18(1):2753234. doi: 10.1080/19420862.2026.2753234. Epub 2026 Oct 29. PMID- 42957425 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2234-7550 (Print) IS - 1225-1585 (Linking) VI - 52 IP - 5 DP - 2026 Oct 31 TI - Development of a standardized documentation template for neck ultrasonography in oral and maxillofacial surgery: a technical note. PG - 291-299 LID - 10.5125/jkaoms.2026.52.5.291 [doi] AB - Neck ultrasonography (US) is widely used for the evaluation of cervical lymph nodes, thyroid abnormalities, salivary gland disease, and other superficial neck lesions. However, variability in documentation may hinder consistent recording and longitudinal comparison, particularly in oral and maxillofacial surgery (OMFS), where repeated outpatient examinations are frequently performed. We developed a standardized documentation template for neck US, intended for future implementation within an electronic medical record system and tailored to routine OMFS practice. Three OMFS clinicians selected the template items through review of established guidelines and existing neck US reporting practices. The proposed template consists of six modules covering general examination information, cervical lymph nodes, diffuse thyroid findings, thyroid nodules, salivary gland assessment, and anatomical lesion mapping. Established sonographic terminology and reporting frameworks were incorporated where applicable, together with fields for previous measurements, procedural history, and interval change. The modular structure was designed to accommodate both comprehensive and focused neck US examinations within the routine OMFS outpatient workflow. This integrated format may provide a practical framework for standardized documentation and longitudinal comparison. Future clinical studies are required to evaluate its usability, reproducibility, and clinical value. FAU - Kong, Ui Hyun AU - Kong UH AUID- ORCID: 0009-0001-6210-0213 AD - Department of Oral and Maxillofacial Surgery, National Cancer Center, Ilsan, Korea. FAU - Do, Jiwon AU - Do J AUID- ORCID: 0000-0002-0973-8410 AD - Department of Oral and Maxillofacial Surgery, Seoul National University Dental Hospital, Seoul, Korea. FAU - Youn, Sung Bin AU - Youn SB AUID- ORCID: 0000-0002-4961-7058 AD - Orthognathic and Facial Contouring Surgery Center, Seoul National University Dental Hospital, Seoul, Korea. FAU - Kwon, Ik-Jae AU - Kwon IJ AUID- ORCID: 0000-0002-8222-2971 AD - Department of Oral and Maxillofacial Surgery, Seoul National University Dental Hospital, Seoul, Korea. FAU - Han, Jeong Joon AU - Han JJ AUID- ORCID: 0000-0001-8975-0198 AD - Department of Oral and Maxillofacial Surgery, Seoul National University Dental Hospital, Seoul, Korea. FAU - Seo, Mi Hyun AU - Seo MH AUID- ORCID: 0000-0001-8220-6480 AD - One-Stop Specialty Center, Seoul National University Dental Hospital, Seoul, Korea. FAU - Yang, Hoonjoo AU - Yang H AUID- ORCID: 0000-0002-4652-7305 AD - Department of Oral and Maxillofacial Surgery, Seoul National University Dental Hospital, Seoul, Korea. FAU - Myoung, Hoon AU - Myoung H AUID- ORCID: 0000-0002-9984-8479 AD - Department of Oral and Maxillofacial Surgery, Seoul National University Dental Hospital, Seoul, Korea. LA - eng PT - Journal Article PL - Korea (South) TA - J Korean Assoc Oral Maxillofac Surg JT - Journal of the Korean Association of Oral and Maxillofacial Surgeons JID - 101580242 OTO - NOTNLM OT - Lymph nodes OT - Neck OT - Salivary glands OT - Thyroid gland OT - Ultrasonography EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 22:12 PHST- 2026/09/03 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 22:12 [entrez] AID - jkaoms.2026.52.5.291 [pii] AID - 10.5125/jkaoms.2026.52.5.291 [doi] PST - ppublish SO - J Korean Assoc Oral Maxillofac Surg. 2026 Oct 31;52(5):291-299. doi: 10.5125/jkaoms.2026.52.5.291. PMID- 42957421 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2234-7550 (Print) IS - 1225-1585 (Linking) VI - 52 IP - 5 DP - 2026 Oct 31 TI - Defect-oriented reconstruction of extensive full-thickness lower lip defects using Fujimori gate flaps and Bernard-Burow-Webster advancement: a four-case series. PG - 259-269 LID - 10.5125/jkaoms.2026.52.5.259 [doi] AB - OBJECTIVES: We describe defect-oriented technique selection and outcomes in all four patients who underwent immediate one-stage reconstruction after wide excision of lower lip squamous cell carcinoma during the study period. PATIENTS AND METHODS: Two total defects with bilateral commissural loss were reconstructed with bilateral Fujimori gate flaps, and two large central full-thickness defects with preserved commissures were reconstructed with bilateral Bernard-Burow-Webster advancement. Clinical records and serial photographs were reviewed. RESULTS: Frozen section and final margins were negative, and all flaps remained viable through the last outpatient follow-up. Median photographic and medical record follow-up periods were 122.5 days (range, 72-244) and 274.5 days (range, 136-758), respectively. No specific reconstructive complication was documented in Case 1. Case 2 had transient decreased sensation in the chin region through 2 weeks and drooling through 1 month, followed by improvement. Case 3 developed lower-lip ptosis, oral incompetence with drooling, marked firmness, restricted mobility, persistent food leakage and retention, and impaired eating. In Case 4, early stiffness, perceived reduction in oral aperture, and eating difficulty gradually improved. No secondary revision was performed. CONCLUSION: Technique selection should consider defect extent and commissure status. Standardized functional assessment was unavailable, and this small, noncomparative series cannot establish superiority. FAU - Park, Sang Hyun AU - Park SH AUID- ORCID: 0009-0008-7320-4393 AD - Oral Oncology Clinic, Research Institute and Hospital, National Cancer Center, Goyang, Korea. FAU - Choi, Yong-Seok AU - Choi YS AUID- ORCID: 0000-0002-4384-0910 AD - Oral Oncology Clinic, Research Institute and Hospital, National Cancer Center, Goyang, Korea. FAU - Lee, Jong-Ho AU - Lee JH AUID- ORCID: 0000-0002-8843-545X AD - Oral Oncology Clinic, Research Institute and Hospital, National Cancer Center, Goyang, Korea. AD - Department of Oral and Maxillofacial Surgery, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Korea. FAU - Choi, Sung Weon AU - Choi SW AUID- ORCID: 0000-0002-2038-2881 AD - Oral Oncology Clinic, Research Institute and Hospital, National Cancer Center, Goyang, Korea. FAU - Park, Joo-Yong AU - Park JY AUID- ORCID: 0000-0002-0969-151X AD - Oral Oncology Clinic, Research Institute and Hospital, National Cancer Center, Goyang, Korea. LA - eng PT - Journal Article PL - Korea (South) TA - J Korean Assoc Oral Maxillofac Surg JT - Journal of the Korean Association of Oral and Maxillofacial Surgeons JID - 101580242 OTO - NOTNLM OT - Bernard-Burow-Webster flap OT - Fujimori gate flap OT - Lip neoplasms OT - Lower lip reconstruction OT - Surgical flaps EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 22:12 PHST- 2026/09/04 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 22:12 [entrez] AID - jkaoms.2026.52.5.259 [pii] AID - 10.5125/jkaoms.2026.52.5.259 [doi] PST - ppublish SO - J Korean Assoc Oral Maxillofac Surg. 2026 Oct 31;52(5):259-269. doi: 10.5125/jkaoms.2026.52.5.259. PMID- 42957414 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1473-2300 (Electronic) IS - 0300-0605 (Linking) VI - 54 IP - 10 DP - 2026 Oct TI - Exploring the association between Zhejiang University index and osteoporosis risk in middle-aged and older adults based on the National Health and Nutrition Examination Survey database. PG - 3000605261477328 LID - 10.1177/03000605261477328 [doi] AB - BackgroundOsteoporosis is a metabolic bone disease characterized by reduced bone density and an increased risk of fractures. Metabolic factors, including body mass index and blood glucose levels, are associated with osteoporosis. The Zhejiang University index, which integrates these factors, may influence the risk of osteoporosis. Using data from the National Health and Nutrition Examination Survey database, this study examined the association between the Zhejiang University index and osteoporosis risk, thereby providing a potential novel indicator and strategy for the early detection and intervention of osteoporosis.MethodsData from the National Health and Nutrition Examination Survey (2007-2008 and 2013-2014) were analyzed, resulting in a final cohort of 1003 participants after accounting for fasting subsample and dual-energy X-ray absorptiometry data constraints. Weighted multivariable logistic regression and restricted cubic splines analyses were used to evaluate the association between the Zhejiang University index and osteoporosis. Several sensitivity analyses were conducted, including redefining the outcome using only dual-energy X-ray absorptiometry-confirmed cases and recalculating the Zhejiang University index without its intrinsic sex adjustment (+2 points for female participants). Variable selection was performed using Least Absolute Shrinkage and Selection Operator regression, and model performance was evaluated using receiver operating characteristic curves.ResultsThe Zhejiang University index was significantly inversely associated with the risk of osteoporosis. In the fully adjusted model, participants in the highest quartile had a significantly lower risk of osteoporosis than those in the lowest quartile (odds ratio = 0.18, 95% confidence interval: 0.11-0.31, p < 0.001). Conversely, when the highest quartile was used as the reference, individuals in the lowest quartile faced a 5.50-fold higher risk of osteoporosis (odds ratio = 5.50, 95% confidence interval: 3.21-9.43). Restricted cubic splines analysis confirmed a linear inverse relationship (p overall < 0.001, p nonlinear = 0.387). Sensitivity analyses demonstrated that the protective association was robust against diagnostic criteria and intrinsic sex weighting. The final 10-variable prediction model achieved an area under the curve of 0.763, demonstrating superior performance compared with models based solely on clinical factors.ConclusionThe Zhejiang University index independently predicts the risk of osteoporosis, and the associated prediction model demonstrated good performance, providing a useful tool for clinical screening. FAU - Gu, Rui AU - Gu R AD - Department of Orthopedics and Rheumatology, Wuxi Affiliated Hospital of Nanjing University of Chinese Medicine, China. RINGGOLD: 485092 FAU - Zhang, Jian AU - Zhang J AD - Nanjing University of Chinese Medicine, China. RINGGOLD: 66478 FAU - Ge, Yanhao AU - Ge Y AD - Nanjing University of Chinese Medicine, China. RINGGOLD: 66478 FAU - Hu, Zhuoyi AU - Hu Z AUID- ORCID: 0000-0002-7776-6704 AD - Nanjing University of Chinese Medicine, China. RINGGOLD: 66478 FAU - Zhang, Yafeng AU - Zhang Y AD - Department of Orthopedics and Rheumatology, Wuxi Affiliated Hospital of Nanjing University of Chinese Medicine, China. RINGGOLD: 485092 AD - Jiangsu CM Clinical Innovation Center of Degenerative Bone & Joint Disease, China. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - J Int Med Res JT - The Journal of international medical research JID - 0346411 SB - IM MH - Humans MH - Female MH - *Osteoporosis/epidemiology/diagnosis MH - Nutrition Surveys MH - Male MH - Middle Aged MH - Bone Density MH - Aged MH - Absorptiometry, Photon MH - Risk Factors MH - China/epidemiology MH - ROC Curve MH - Body Mass Index MH - Databases, Factual MH - Logistic Models OTO - NOTNLM OT - National Health and Nutrition Examination Survey OT - Zhejiang University index OT - logistic regression OT - osteoporosis OT - risk prediction COIS- Conflict of interest statementThe authors declare that the research was conducted in the absence of any commercial or fi nancial relationships that could be construed as a potential con fl ict of interest. EDAT- 2026/10/29 23:28 MHDA- 2026/10/30 05:30 CRDT- 2026/10/29 22:12 PHST- 2026/10/30 05:30 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 22:12 [entrez] AID - 10.1177/03000605261477328 [doi] PST - ppublish SO - J Int Med Res. 2026 Oct;54(10):3000605261477328. doi: 10.1177/03000605261477328. Epub 2026 Oct 29. PMID- 42957412 OWN - NLM STAT- Publisher LR - 20261029 IS - 1469-185X (Electronic) IS - 0006-3231 (Linking) DP - 2026 Oct 29 TI - Ecological risks of using non-native freshwater fish for biological control. LID - 10.1002/brv.70216 [doi] AB - The use of freshwater fish as biological control agents has been widely implemented to manage populations of perceived nuisance species, ranging from disease vectors to aquatic weeds. While these introductions are often intended as a management tool, they can have unintended and severe ecological, economic, and social consequences. This review assesses the risks associated with using non-native fish for biological control, emphasising the challenges presented by their persistence, adaptability, and interactions with native species and ecosystems. Key case studies illustrate how species such as mosquitofishes (Gambusia spp.), grass carp (Ctenopharyngodon idella), and tilapias (mainly Oreochromis spp.) have inadvertently contributed to biodiversity loss, habitat degradation, trophic disruptions, and disease transmission, while often failing to effectively control the target species. We further explore the limitations of biocontrol strategies, including the context-dependent nature of species interactions and deferred negative impacts. Given the complexity and unpredictability of biological control interventions, rigorous impact assessments and long-term monitoring programmes are needed before and after introducing non-native fish as control agents, embedded within an adaptive management framework that allows for responsive decision-making based on observed ecological outcomes. Precautionary policies should prioritise habitat restoration and native species-based solutions over the introduction of non-native species to support more sustainable and ecologically responsible management decisions. CI - (c) 2026 Cambridge Philosophical Society. FAU - Franco, Ana Clara Sampaio AU - Franco ACS AUID- ORCID: 0000-0002-1572-3645 AD - GRECO, Institute of Aquatic Ecology, University of Girona, Girona, Catalonia, 17003, Spain. FAU - Azevedo-Santos, Valter M AU - Azevedo-Santos VM AUID- ORCID: 0000-0001-8986-6406 AD - Programa de Pos-Graduacao em Biodiversidade, Ecologia e Conservacao, Universidade Federal do Tocantins, TO, Brazil. AD - Programa de Pos-Graduacao em Ciencias Ambientais, Universidade Federal do Maranhao, Chapadinha, Maranhao, Brazil. FAU - Vitule, Jean R S AU - Vitule JRS AUID- ORCID: 0000-0001-6543-7439 AD - Laboratorio de Ecologia e Conservacao, Departamento de Engenharia Ambiental, Setor de Tecnologia, Universidade Federal do Parana, Centro Politecnico, Curitiba, Parana, Brazil. FAU - Haubrock, Phillip J AU - Haubrock PJ AUID- ORCID: 0000-0003-2154-4341 AD - Faculty of Health, Environment and Medical Sciences, Bournemouth University, Poole, Dorset, BH12 5BB, UK. AD - Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany, 389 01, Czech Republic. AD - Department of Mathematics and Natural Sciences, Gulf University for Science and Technology, P.O. Box 7207, Hawally, 32093, Kuwait. FAU - Macedo, Rafael Lacerda AU - Macedo RL AUID- ORCID: 0000-0001-5086-0170 AD - Institute of Biology, Freie Universitat Berlin, Konigin-Luise-Str. 1-3, Berlin, 14195, Germany. AD - Leibniz Institute of Freshwater Ecology and Inland Fisheries, Muggelseedamm 310, Berlin, 12587, Germany. FAU - Soto, Ismael AU - Soto I AUID- ORCID: 0000-0002-7288-6336 AD - Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany, 389 01, Czech Republic. AD - Department of Conservation Biology and Global Change, Donana Biological Station, Spanish National Research Council, Seville, Spain. FAU - Everts, Teun AU - Everts T AUID- ORCID: 0000-0001-7862-4209 AD - Research Institute for Nature and Forest, Geraardsbergen, Belgium. AD - KU Leuven, Heverlee, Belgium. FAU - Sousa, Ronaldo AU - Sousa R AUID- ORCID: 0000-0002-5961-5515 AD - Centre for Molecular and Environmental Biology/Aquatic Research Network & Institute of Science and Innovation for Bio-Sustainability, Department of Biology, University of Minho, 8 Campus Gualtar, Braga, 4710-057, Portugal. FAU - Petry, Ana Cristina AU - Petry AC AUID- ORCID: 0000-0001-9039-2738 AD - Instituto de Biodiversidade e Sustentabilidade, Universidade Federal do Rio de Janeiro, Av. Amaro Reinaldo dos Santos Silva, 764, Macae 27965-045, Rio de Janeiro, Brazil. FAU - Pelicice, Fernando M AU - Pelicice FM AUID- ORCID: 0000-0002-9700-1194 AD - Programa de Pos-Graduacao em Biodiversidade, Ecologia e Conservacao, Universidade Federal do Tocantins, TO, Brazil. FAU - Tarkan, Ali Serhan AU - Tarkan AS AUID- ORCID: 0000-0001-8628-0514 AD - Department of Mathematics and Natural Sciences, Gulf University for Science and Technology, P.O. Box 7207, Hawally, 32093, Kuwait. AD - Department of Ecology and Vertebrate Zoology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland. AD - Department of Basic Sciences, Faculty of Fisheries, Mugla Sitki Kocman University, Mugla, Turkiye. FAU - Manna, Luisa Resende AU - Manna LR AUID- ORCID: 0000-0003-4223-8466 AD - Departamento de Ecologia, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil. FAU - Daga, Vanessa S AU - Daga VS AUID- ORCID: 0000-0003-3707-716X AD - Independent Researcher, Deltona, FL, 32725, USA. FAU - Olden, Julian D AU - Olden JD AUID- ORCID: 0000-0003-2143-1187 AD - School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA, 98105, USA. FAU - Britton, J Robert AU - Britton JR AUID- ORCID: 0000-0003-1853-3086 AD - Faculty of Health, Environment and Medical Sciences, Bournemouth University, Poole, Dorset, BH12 5BB, UK. FAU - Kouba, Antonin AU - Kouba A AD - Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, University of South Bohemia in Ceske Budejovice, Vodnany, 389 01, Czech Republic. FAU - Vilizzi, Lorenzo AU - Vilizzi L AUID- ORCID: 0000-0001-8103-885X AD - Department of Ecology and Vertebrate Zoology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland. FAU - Magurran, Anne E AU - Magurran AE AUID- ORCID: 0000-0002-0036-2795 AD - Centre for Biological Diversity, School of Biology, University of St Andrews, St Andrews, Scotland, UK. FAU - Arthington, Angela H AU - Arthington AH AUID- ORCID: 0000-0001-5967-7954 AD - Australian Rivers Institute, Griffith University, 170 Kessels Road, Nathan, Queensland, 4111, Australia. FAU - Garcia-Berthou, Emili AU - Garcia-Berthou E AUID- ORCID: 0000-0001-8412-741X AD - GRECO, Institute of Aquatic Ecology, University of Girona, Girona, Catalonia, 17003, Spain. FAU - Simberloff, Daniel AU - Simberloff D AD - Department of Ecology and Evolutionary Biology, University of Tennessee, Knoxville, TN, 37996, USA. LA - eng GR - MCIN/AEI/10.13039/501100011033/Ministerio de Ciencia e Innovacion/ GR - MICIU/AEI/10.13039/501100011033/Ministerio de Ciencia e Innovacion/ GR - PID2019-103936GB-C21/NextGenerationEU/PRTR/ GR - TED2021-129889B-I00/NextGenerationEU/PRTR/ GR - RED2022-134338-T/NextGenerationEU/PRTR/ GR - PID2023-146173NB-C21/NextGenerationEU/PRTR/ GR - 315924/2023-3/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ GR - 312702/2026-4/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ GR - 310471/2023-0/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ GR - E-26/010.001930/2019/Fundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro/ GR - E-26/210.557/2024/Fundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro/ GR - E-26/200.576/2025/Fundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro/ GR - 101203662/European Union's Horizon Europe research and innovation programme under the Marie Sklodowska-Curie/ GR - COMPETE2030-FEDER-00686700/Fundacao para a Ciencia e a Tecnologia/ GR - 1S01822N/Research Foundation Flanders/ GR - CEX2024-001498-S/Agencia Estatal de Investigacion/ GR - Spanish Ministry of Science and Innovation/ GR - Portuguese Foundation for Science and Technology/ PT - Journal Article DEP - 20261029 PL - England TA - Biol Rev Camb Philos Soc JT - Biological reviews of the Cambridge Philosophical Society JID - 0414576 SB - IM OTO - NOTNLM OT - biomanipulation OT - conservation management OT - freshwater ecosystems OT - invasive species OT - trophic cascades EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 22:12 PHST- 2026/10/07 00:00 [revised] PHST- 2025/10/13 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 22:12 [entrez] AID - 10.1002/brv.70216 [doi] PST - aheadofprint SO - Biol Rev Camb Philos Soc. 2026 Oct 29. doi: 10.1002/brv.70216. PMID- 42957402 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1557-8275 (Electronic) IS - 0033-8389 (Linking) VI - 64 IP - 6 DP - 2026 Nov TI - Novel Radiotracers in PET Imaging. PG - 1167-1184 LID - S0033-8389(26)00118-1 [pii] LID - 10.1016/j.rcl.2026.08.011 [doi] AB - This article reviews emerging PET radiopharmaceuticals in late preclinical and early clinical development. The discussed tracers have broad applications for disease staging, theranostic patient selection, and treatment monitoring. For oncology, tracers targeting fibroblast activation protein, prostate-specific membrane antigen, delta-like ligand 3, trophoblast cell-surface antigen 2, claudin 18.2, and amino-acid transporters are highlighted alongside immune-targeted tracers imaging cluster of differentiation proteins, the programmed death-1 receptor and ligand, granzyme B, and macrophages. In neurology, tracers targeting amyloid-beta, tau, translocator protein, and synaptic vesicle protein 2A are discussed. Highlighted cardiology tracers focus on imaging of myocardial perfusion, amyloidosis, and vascular inflammation. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Gimblet, Grayson R AU - Gimblet GR AD - Department of Radiology, University of Alabama at Birmingham, 1824 6th Avenue S, WTI 310A, Birmingham, AL 35294, USA. FAU - Lapi, Suzanne E AU - Lapi SE AD - Department of Radiology, University of Alabama at Birmingham, 1824 6th Avenue S, WTI 310A, Birmingham, AL 35294, USA. Electronic address: lapi@uab.edu. LA - eng PT - Journal Article PT - Review DEP - 20260923 PL - United States TA - Radiol Clin North Am JT - Radiologic clinics of North America JID - 0123703 RN - 0 (Radiopharmaceuticals) SB - IM MH - Humans MH - *Radiopharmaceuticals MH - *Positron-Emission Tomography/methods MH - *Neoplasms/diagnostic imaging OTO - NOTNLM OT - Imaging OT - PET OT - Radiopharmaceutical OT - Tracer COIS- Disclosures The authors have nothing to disclose. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 21:55 PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 21:55 [entrez] AID - S0033-8389(26)00118-1 [pii] AID - 10.1016/j.rcl.2026.08.011 [doi] PST - ppublish SO - Radiol Clin North Am. 2026 Nov;64(6):1167-1184. doi: 10.1016/j.rcl.2026.08.011. Epub 2026 Sep 23. PMID- 42957400 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1557-8275 (Electronic) IS - 0033-8389 (Linking) VI - 64 IP - 6 DP - 2026 Nov TI - PET in Theranostics: Patient Selection, Treatment Planning, and Response Assessment. PG - 1127-1144 LID - S0033-8389(26)00119-3 [pii] LID - 10.1016/j.rcl.2026.08.012 [doi] AB - Theranostics pairs molecular imaging with a therapeutic radiopharmaceutical directed at the same target, with PET assessing target expression, disease extent, interlesional heterogeneity, and FDG discordance. Three established applications are reviewed: PSMA PET-guided selection for lutetium-177-PSMA-617 in metastatic androgen pathway modulation-resistant prostate cancer, somatostatin receptor PET-guided peptide receptor radionuclide therapy in neuroendocrine tumors, and iodine-124/FDG PET within radioiodine therapy for differentiated thyroid cancer. Practical guidance covers patient selection, image interpretation, treatment planning, response assessment, and reporting. A key paradigm: diagnostic uptake confirms target availability but neither measures delivered dose nor guarantees response; biomarkers, dosimetry, and response frameworks remain variably validated. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Veziroglu, Eren AU - Veziroglu E AD - Department of Radiology, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA; Institute of Nuclear Medicine, Bethesda, MD, USA. FAU - Gifani, Mahsa AU - Gifani M AD - Department of Obstetrics, Gynecology and Reproductive Biology, College of Human Medicine, Michigan State University, Grand Rapids, MI, USA. FAU - Nikpanah, Moozhan AU - Nikpanah M AD - Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. FAU - Gowda, Vrushab AU - Gowda V AD - Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. FAU - Farhadi, Faraz AU - Farhadi F AD - Institute of Nuclear Medicine, Bethesda, MD, USA; Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA. FAU - Saboury, Babak AU - Saboury B AD - Institute of Nuclear Medicine, Bethesda, MD, USA. Electronic address: bsaboury@unithera.com. LA - eng PT - Journal Article PT - Review PL - United States TA - Radiol Clin North Am JT - Radiologic clinics of North America JID - 0123703 RN - 0 (Radiopharmaceuticals) SB - IM MH - Humans MH - Radiopharmaceuticals MH - *Positron-Emission Tomography/methods MH - *Patient Selection MH - *Theranostic Nanomedicine/methods MH - Precision Medicine/methods OTO - NOTNLM OT - PET OT - Patient selection OT - Radiopharmaceutical therapy (RPT) OT - Theranostics EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 21:55 PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 21:55 [entrez] AID - S0033-8389(26)00119-3 [pii] AID - 10.1016/j.rcl.2026.08.012 [doi] PST - ppublish SO - Radiol Clin North Am. 2026 Nov;64(6):1127-1144. doi: 10.1016/j.rcl.2026.08.012. PMID- 42957345 OWN - NLM STAT- In-Process LR - 20261029 IS - 1757-790X (Electronic) IS - 1757-790X (Linking) VI - 19 IP - 10 DP - 2026 Oct 29 TI - Radiographically occult invasive lobular carcinoma presenting as anaemia and thrombocytopenia. LID - e275988 [pii] LID - 10.1136/bcr-2026-275988 [doi] FAU - Sachdev, Koushalya AU - Sachdev K AD - Department of Internal Medicine, The University of Texas at Austin Dell Medical School, Austin, Texas, USA koushalya.sachdev@austin.utexas.edu. FAU - Sheng, Angela AU - Sheng A AD - Division of Hematology & Oncology, The University of Texas at Austin Dell Medical School, Austin, Texas, USA. FAU - Goodgame, Boone AU - Goodgame B AD - Division of Hematology & Oncology, The University of Texas at Austin Dell Medical School, Austin, Texas, USA. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - BMJ Case Rep JT - BMJ case reports JID - 101526291 SB - IM OTO - NOTNLM OT - Cancer - see Oncology OT - Haematology (incl blood transfusion) COIS- Competing interests: None declared. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 21:32 PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 21:32 [entrez] AID - 19/10/e275988 [pii] AID - 10.1136/bcr-2026-275988 [doi] PST - epublish SO - BMJ Case Rep. 2026 Oct 29;19(10):e275988. doi: 10.1136/bcr-2026-275988. PMID- 42957342 OWN - NLM STAT- Publisher LR - 20261029 IS - 2374-4243 (Electronic) IS - 2374-4243 (Linking) DP - 2026 Oct 29 TI - Shifting viral aetiology, codetection and age-specific mortality in severe acute respiratory infection in Brazil, 2022-2025: a nationwide surveillance study. PG - 1-18 LID - 10.1080/23744235.2026.2754995 [doi] AB - BACKGROUND: Respiratory viral aetiology and codetection among severe acute respiratory infection (SARI) notifications have evolved post-COVID-19. We examined temporal patterns, age heterogeneity, and severe outcome associations in Brazil. METHODS: We analysed nationwide SIVEP-Gripe SARI records (2022-2025; extracted July 2026) with RT-PCR viral detection. Seven mutually exclusive categories were evaluated using robust Poisson regression to estimate adjusted risk ratios (aRRs) for SARI-attributed mortality and a severe composite outcome (ICU admission, invasive ventilation, or death). RESULTS: Among 447,107 virus-positive records, 7.2% involved codetections and 25.4% included RSV. Isolated SARS-CoV-2 declined from 72.4% (2022) to 6.8% (2025), while codetections rose from 3.0% to 11.2%. Compared to isolated RSV, mortality was higher for codetection without RSV (aRR 1.80, 95% CI: 1.67-1.94), isolated SARS-CoV-2 (1.62, 1.54-1.70), and codetection with RSV (1.40, 1.27-1.55). Associations varied by age ($p < .001$). Among RSV-positive cases, codetection conferred increased mortality risk (aRR 1.46, 1.32-1.61). Excluding isolated SARS-CoV-2 attenuated the 2025-versus-2022 codetection prevalence ratio from 1.85 to 1.10. CONCLUSIONS: Viral detections shifted towards greater heterogeneity, strongly driven by changing surveillance composition. Varying testing indications and panels limit causal inference. Integrated, age-sensitive surveillance with standardized diagnostic reporting is essential; surveillance proportions do not reflect population incidence. FAU - Oliveira Andrade, Natasha Cristina AU - Oliveira Andrade NC AD - Programa de Pos-Graduacao em Biologia Parasitaria na Amazonia, Universidade do Estado do Para, and Instituto Evandro Chagas (PPGBPA/UEPA/IEC), Belem, Para, Brazil. FAU - Socorro Dos Santos, Suziane do AU - Socorro Dos Santos SD AD - Programa de Pos-Graduacao em Biologia Parasitaria na Amazonia, Universidade do Estado do Para, and Instituto Evandro Chagas (PPGBPA/UEPA/IEC), Belem, Para, Brazil. FAU - de Nazare Soares, Tamires AU - de Nazare Soares T AD - Programa de Pos-Graduacao em Biologia Parasitaria na Amazonia, Universidade do Estado do Para, and Instituto Evandro Chagas (PPGBPA/UEPA/IEC), Belem, Para, Brazil. FAU - Abrahao Silva, Marcos Jesse AU - Abrahao Silva MJ AD - Programa de Pos-Graduacao em Biologia Parasitaria na Amazonia, Universidade do Estado do Para, and Instituto Evandro Chagas (PPGBPA/UEPA/IEC), Belem, Para, Brazil. FAU - Lobato Marinho, Rebecca AU - Lobato Marinho R AD - Programa de Pos-Graduacao em Biologia Parasitaria na Amazonia, Universidade do Estado do Para, and Instituto Evandro Chagas (PPGBPA/UEPA/IEC), Belem, Para, Brazil. FAU - Faine Freitas Takeda, Karina AU - Faine Freitas Takeda K AD - Programa de Pos-Graduacao em Ensino em Saude na Amazonia, Universidade do Estado do Para (PPGESA/UEPA), Belem, Para, Brazil. FAU - Sardinha, Daniele Melo AU - Sardinha DM AD - Programa de Pos-Graduacao em Biologia Parasitaria na Amazonia, Universidade do Estado do Para, and Instituto Evandro Chagas (PPGBPA/UEPA/IEC), Belem, Para, Brazil. AD - Programa de Pos-Graduacao em Epidemiologia e Vigilancia em Saude, Instituto Evandro Chagas (PPGEVS/IEC), Ananindeua, Para, Brazil. FAU - Nepomuceno Gondim Costa Lima, Luana AU - Nepomuceno Gondim Costa Lima L AD - Programa de Pos-Graduacao em Biologia Parasitaria na Amazonia, Universidade do Estado do Para, and Instituto Evandro Chagas (PPGBPA/UEPA/IEC), Belem, Para, Brazil. AD - Programa de Pos-Graduacao em Epidemiologia e Vigilancia em Saude, Instituto Evandro Chagas (PPGEVS/IEC), Ananindeua, Para, Brazil. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Infect Dis (Lond) JT - Infectious diseases (London, England) JID - 101650235 SB - IM OTO - NOTNLM OT - COVID-19 OT - mortality OT - respiratory syncytial virus OT - severe acute respiratory infection OT - viral codetection EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 21:32 PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 21:32 [entrez] AID - 10.1080/23744235.2026.2754995 [doi] PST - aheadofprint SO - Infect Dis (Lond). 2026 Oct 29:1-18. doi: 10.1080/23744235.2026.2754995. PMID- 42957335 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1001-9391 (Print) IS - 1001-9391 (Linking) VI - 44 IP - 10 DP - 2026 Oct 20 TI - [Analysis of liver and lung function status among workers exposed to isocyanates and other occupational hazards]. PG - 847-852 LID - 10.3760/cma.j.cn121094-20250225-00077 [doi] AB - Objective: To analyze the current status of pulmonary and liver function in workers exposed to isocyanates and other hazardous factors in Shanghai city, and to evaluate the association between occupational exposure and health status. Methods: In February 2025, based on the occupational health examination data from 2013 to 2021 of an occupational health examination institution in the Shanghai occupational health examination database, workers whose hazardous factor exposure records included isocyanates (isocyanates, toluene diisocyanate, diphenylmethane diisocyanate, hexamethylene diisocyanate and isophorone diisocyanate) were selected as study subjects. The pulmonary and liver function at the last examination were analyzed, with the workers grouped by the toxicity of other co-exposed chemicals. Differences in health examination indices among the pre-employment examinees and the on-the-job workers exposed to different types of toxicants were compared using chi-square test, Wilcoxon signed-rank test, Kruskal-Wallis test and Dunn test. The effects of length of service on the health status of the on-the-job examinees were analyzed using multivariate logistic regression and multiple linear regression models. Results: A total of 37875 workers from 192 enterprises were included, including 24362 pre-employment examinees and 13513 on-the-job examinees; among the on-the-job workers, 1312 were exposed to isocyanates only, 9736 were concurrently exposed to hepatotoxic chemicals, and 9830 were concurrently exposed to respiratory toxicants. Compared with the pre-employment examinees, the on-the-job workers concurrently exposed to hepatotoxic or non-hepatotoxic chemicals had lower levels of alkaline phosphatase (ALP) and albumin (ALB), higher rates of hepatic fatty infiltration, fatty liver and abnormal liver B-ultrasound (P<0.001), and the male workers had higher levels of alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), total bilirubin (TBIL) and direct bilirubin (DBIL) (P<0.001) ; the levels of ALP, total protein (TP) and ALB were lower in the workers concurrently exposed to non-hepatotoxic chemicals than in those concurrently exposed to hepatotoxic chemicals (P<0.001). With the increase of length of service, the workers concurrently exposed to hepatotoxic chemicals showed increased levels of ALT, GGT, TBIL and DBIL, decreased levels of ALP and ALB, and increased risks of fatty liver and abnormal liver B-ultrasound (P<0.05), and the risk of abnormal liver B-ultrasound increased in the workers concurrently exposed to non-hepatotoxic chemicals (P=0.002). Compared with the pre-employment examinees, the on-the-job examinees had higher average levels of forced vital capacity as predicted value percentage (FVC%pred), forced expiratory volume in the first second as predicted value percentage (FEV(1)%pred) and FEV(1)/FVC, as well as a higher detection rate of abnormal chest radiographs (P<0.001). The FEV(1)%pred of the workers exposed to isocyanates only was lower than that of the workers concurrently exposed to other toxicants (P<0.001) ; with the increase of length of service, the FVC%pred and FEV(1)%pred of the workers concurrently exposed to other toxicants showed upward trends, while FEV(1)/FVC showed a downward trend (P<0.001) . Conclusion: Slight liver function impairment exists in the on-the-job workers exposed to isocyanates. With the increase of length of service, workers co-exposed to other hazardous factors may have a slight decline in pulmonary ventilation function, and the workers exposed to hepatotoxic chemicals have an increased risk of liver abnormality. FAU - Cao, C H AU - Cao CH AD - School of Public Health, Fudan University, Shanghai 200032, China. FAU - Zhang, J M AU - Zhang JM AD - Key Laboratory of Public Health and Safety, Ministry of Education, Fudan University, Shanghai 200032, China. FAU - Zhang, Q Y AU - Zhang QY AD - School of Public Health, Fudan University, Shanghai 200032, China Shanghai Municipal Center for Disease Prevention and Control, Shanghai 200336, China. FAU - Duan, Z P AU - Duan ZP AD - School of Public Health, Fudan University, Shanghai 200032, China Shanghai Institute for Prevention and Treatment of Occupational Diseases in the Chemical Industry, Shanghai 200041, China. FAU - Zhou, Z J AU - Zhou ZJ AD - School of Public Health, Fudan University, Shanghai 200032, China Key Laboratory of Public Health and Safety, Ministry of Education, Fudan University, Shanghai 200032, China. LA - chi GR - GWVI-11.1-37, GWVI-4/The Three-Year Action Plan for Public Health in Shanghai/ GR - 2024GKQ04/The Special Program for Public Health Research in Shanghai/ GR - 23YF1407700/Shanghai Sailing Program for Young Scientists and Technologists/ PT - English Abstract PT - Journal Article PL - China TA - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi JT - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases JID - 8410840 RN - 0 (Isocyanates) SB - IM MH - Humans MH - *Occupational Exposure MH - *Isocyanates/adverse effects MH - Male MH - Adult MH - *Liver/physiopathology MH - *Lung/physiopathology MH - Middle Aged MH - Liver Function Tests MH - Female MH - Respiratory Function Tests OTO - NOTNLM OT - Isocyanates OT - Liver OT - Liver function tests OT - Occupational diseases OT - Occupational exposure OT - Respiratory function tests OT - Toluene 2, 4-diisocyanate COIS- 所有作者均声明不存在利益冲突 EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 21:28 PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 21:28 [entrez] AID - 10.3760/cma.j.cn121094-20250225-00077 [doi] PST - ppublish SO - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2026 Oct 20;44(10):847-852. doi: 10.3760/cma.j.cn121094-20250225-00077. PMID- 42957333 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1001-9391 (Print) IS - 1001-9391 (Linking) VI - 44 IP - 10 DP - 2026 Oct 20 TI - [Occupational exposure levels of hand-transmitted vibration and vibration reduction measures in handheld workpiece grinding operations]. PG - 837-841 LID - 10.3760/cma.j.cn121094-20250605-00224 [doi] AB - Objective: To study the occupational exposure levels of hand-transmitted vibration in handheld workpiece grinding operations and the vibration reduction measures, in order to provide scientific basis for reducing hand-transmitted vibration hazards in handheld workpiece grinding operations. Methods: From April to December 2022, 34 handheld workpiece grinding operation positions in a metal manufacturing enterprise in Guangdong Province were selected using the judgment sampling method for hand-transmitted vibration measurement. The frequency-weighted vibration acceleration values (a(hwx), a(hwy), a(hwz)) along the X, Y, and Z axes of each operation position were compared using the Friedman test. Four experiments were conducted: dynamic balance improvement of the grinding machine's driving wheel, relative angle correction between the driving wheel and driven wheel, vibration reduction improvement of the machine base, and testing of different grinding belts. Variance analysis or linear mixed models were employed to analyze and compare the differences in the total vibration values (a(hv)) of hand-transmitted vibration frequency-weighted root mean square acceleration of different improvement measures. Results: For the handheld workpiece grinding operation positions, the M (P(0), P(100)) of a(hwx) was 3.15 (1.19, 9.24) m/s(2), a(hwy) was 3.01 (1.47, 11.70) m/s(2), both of which were significantly higher than a(hwz)[2.58 (0.89, 8.56) m/s(2)] (P<0.05). The M (P(0), P(100)) of the 8-hour energy-equivalent frequency-weighted vibration acceleration[A (8) ] values of the positions was 4.93 (2.51, 14.54) m/s(2) with 17 operation posts (50.0%) exceeding 5 m/s(2). Across five eccentric mass groups (with eccentric masses of 3, 10, 15, 22, and 32 g), the a(hv) values were (1.94+/-0.22), (4.51+/-0.51), (3.92+/-0.48), (7.34+/-0.62), and (11.65+/-2.18) m/s(2), respectively. The higher eccentric mass of the driving wheel correlated with a larger a(hv) (P<0.001). Under different relative positions of the front and rear wheels (angles of <1 degrees and 5 degrees ), the a(hv) values were (3.01+/-0.34) and (3.07+/-0.37) m/s(2), showing no statistically significant difference (P=0.628). The a(hv) when using a double-layer rubber seat pad for operation was (7.22+/-0.58) m/s(2), which was lower than that of a single-layer rubber seat pad[ (7.77+/-0.50) m/s(2)] (P=0.012). When grinding the same number of workpieces, the a(hv) of 50 mesh belt[2.98 (2.05,7.68) m/s(2)] was lower than that of 60 mesh belt[4.29 (2.17, 8.38) m/s(2)] (P=0.023) . Conclusion: The hand-transmitted vibration level in handheld workpiece grinding operations is relatively high. By correcting the eccentric mass of the driving wheel of the grinding machine, installing double-layer rubber pads, and selecting appropriate grinding sand belts, the hand-transmitted vibration exposure level can be reduced. FAU - Xiao, B AU - Xiao B AD - Institute of Physical Factor Surveillance, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China. FAU - Jiang, Y J AU - Jiang YJ AD - Institute of Physical Factor Surveillance, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China. FAU - Yan, M S AU - Yan MS AD - Institute of Physical Factor Surveillance, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China. FAU - Guo, J Y AU - Guo JY AD - Institute of Physical Factor Surveillance, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China. FAU - Wu, J L AU - Wu JL AD - Institute of Physical Factor Surveillance, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China. FAU - Wen, W AU - Wen W AD - Institute of Occupational Health Assessment, Guangdong Province Hospital for Occupational Disease Prevention and Treatment, Guangzhou 510300, China. LA - chi GR - 2011-09/National Clinical Key Specialty Construction Project/ GR - B2023326/Medical Science and Technology Research Fund of Guangdong Province/ PT - English Abstract PT - Journal Article PL - China TA - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi JT - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases JID - 8410840 SB - IM MH - *Vibration MH - *Occupational Exposure/prevention & control/analysis MH - Humans MH - Working Conditions MH - Hand MH - Hand-Arm Vibration Syndrome/prevention & control OTO - NOTNLM OT - Hand-arm vibration disease OT - Hand-transmitted vibration OT - Occupational exposure OT - Vibration reduction measures COIS- 所有作者均声明不存在利益冲突 EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 21:28 PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 21:28 [entrez] AID - 10.3760/cma.j.cn121094-20250605-00224 [doi] PST - ppublish SO - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2026 Oct 20;44(10):837-841. doi: 10.3760/cma.j.cn121094-20250605-00224. PMID- 42957330 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1001-9391 (Print) IS - 1001-9391 (Linking) VI - 44 IP - 10 DP - 2026 Oct 20 TI - [Analysis of associated factors for occupational hearing loss and construction of a machine learning prediction model]. PG - 816-823 LID - 10.3760/cma.j.cn121094-20250609-00229 [doi] AB - Objective: To investigate the associated factors of hearing loss among occupational populations and to develop a machine learning predictive model to serve as a reference for risk screening of occupational hearing loss. Methods: In March 2025, based on health examination data from 7969 occupational workers in Zhongshan City in 2020, single-factor analysis, the Boruta algorithm, LASSO regression, and multivariate logistic regression were employed to identify and analyze factors associated with hearing loss. Six predictive models-logistic regression, naive Bayes, decision tree, random forest, Bayesian network, and extreme gradient boosting (XGBoost) -were constructed. The optimal model was selected through comprehensive evaluation of discrimination and calibration performance, and the (Shapley additive explanations, SHAP) method was utilized to interpret feature importance. Results: Among the 7969 occupational workers individuals, hearing loss was detected in 39.0% of cases. Multivariate logistic regression analysis revealed that the risk of hearing loss was lower in females than in males (OR=0.40, 95%CI: 0.33-0.48, P<0.001) ; compared to the <30-year-old group, the risk of hearing loss was elevated in the 40-49-year-old group (OR=2.49, 95%CI: 2.17-2.86, P<0.001) ; both noise exposure (OR=1.70, 95%CI: 1.36-2.12, P<0.001) and exposure to welding fumes (OR=1.68, 95%CI: 1.46-1.93, P<0.001) were identified as significant exposure factors for hearing loss. Among the six models, the XGBoost model demonstrated the best performance in terms of accuracy (66.23%), precision (57.53%), and area under the curve (AUC) =0.701. Conclusion: Age, noise exposure, welding fume exposure, and sex were significantly associated with hearing loss. The XGBoost model showed relatively good discriminative ability for predicting hearing loss risk, indicating its preliminary potential for screening high-risk occupational groups. However, due to the cross-sectional design, causal relationships need to be verified in future studies. FAU - Liu, R H AU - Liu RH AD - School of Public Health, Guangdong Medical University, Dongguan 523808, China. FAU - Du, Y X AU - Du YX AD - School of Public Health, Guangdong Medical University, Dongguan 523808, China. FAU - Huang, C W AU - Huang CW AD - Jiangmen Central Hospital, Jiangmen 529000, China. FAU - Chen, J L AU - Chen JL AD - School of Public Health, Guangdong Medical University, Dongguan 523808, China. FAU - Zhang, B S AU - Zhang BS AD - School of Public Health, Guangdong Medical University, Dongguan 523808, China. LA - chi GR - 82304253/National Natural Science Foundation of China Youth Science Fund/ GR - 2022KQNCX021/Guangdong Province Youth Innovation Talent Project for Ordinary Universities/ GR - GDMUB2022054/Guangdong Medical University Doctoral Initiation Project/ PT - English Abstract PT - Journal Article PL - China TA - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi JT - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases JID - 8410840 SB - IM MH - Humans MH - Predictive Learning Models MH - Male MH - *Machine Learning MH - Female MH - Boosting Machine Learning Algorithms MH - Random Forest MH - Logistic Models MH - *Hearing Loss MH - Risk Factors MH - *Occupational Diseases MH - Prediction Algorithms MH - Classification Algorithms MH - Bayes Theorem MH - Occupational Exposure MH - Hearing Loss, Noise-Induced MH - Middle Aged OTO - NOTNLM OT - Boruta OT - Hearing loss OT - Least absolute shrinkage and selection operator (LASSO) regression OT - Machine learning models OT - Noise-induced hearing loss COIS- 所有作者均声明不存在利益冲突 EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 21:28 PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 21:28 [entrez] AID - 10.3760/cma.j.cn121094-20250609-00229 [doi] PST - ppublish SO - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2026 Oct 20;44(10):816-823. doi: 10.3760/cma.j.cn121094-20250609-00229. PMID- 42957327 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1001-9391 (Print) IS - 1001-9391 (Linking) VI - 44 IP - 10 DP - 2026 Oct 20 TI - [Association between neck ergonomic load, job fatigue and work-related musculoskeletal disorders]. PG - 793-798 LID - 10.3760/cma.j.cn121094-20250815-00332 [doi] AB - Objective: To explore the relationship between neck ergonomic load, job fatigue and work-related musculoskeletal disorders (WMSDs), and to provide a scientific basis for formulating preventive measures for WMSDs in the neck. Methods: From January 2018 to December 2023, a stratified cluster sampling method was adopted to select front-line workers from over 400 enterprises of different scales in 22 provinces and cities across 7 geographical regions in China as the research subjects. The "Chinese version of Musculoskeletal Disorders Questionnaire" was used to conduct an epidemiological investigation on neck WMSDs nationwide, collecting information such as general characteristics, neck ergonomic load, neck fatigue, and the occurrence of WMSDs. A total of 83006 participants were included in the study. Multivariate logistic regression analysis was used to analyze the influencing factors of neck WMSDs occurrence, and the Bootstrap mediation analysis method was used to explore the mediating effect of fatigue between ergonomic load and neck WMSDs. Results: The incidence of neck fatigue among workers was 35.70% (29630 people), and the annual incidence of WMSDs was 22.32% (18530 people). The annual incidence of neck WMSDs among workers with neck fatigue was 45.79% (13568/29630), significantly higher than that of non-fatigued workers (9.30%, 4962/53376) (P<0.001). The results of multivariate logistic regression analysis showed that poor neck posture (slightly forward tilt, significant forward tilt, and backward tilt), maintaining the same posture for a long time, maintaining a head-down posture for a long time, maintaining a head-turning posture for a long time, and performing repetitive operations multiple times per minute significantly increased the risk of neck WMSDs (P<0.001) ; workers with job fatigue evaluated as "a little tiring" or above had a significantly increased risk of neck WMSDs (P<0.001). The results of mediating effect analysis showed that neck posture, maintaining the same posture for a long time, maintaining a head-down posture for a long time, maintaining a head-turning posture for a long time, and performing repetitive operations multiple times per minute all had direct effects on neck WMSDs, with effect values of 0.432, 0.574, 0.505, 0.231, and 0.424, respectively; neck fatigue played a partial mediating role between the above ergonomic load and neck WMSDs, and the indirect effect composition ratios were 22.02%, 29.40%, 26.92%, 23.26%, and 24.15%, respectively. Conclusion: The occurrence of neck WMSDs among workers is related to poor neck posture and repetitive operations; fatigue plays a mediating role in the relationship between poor neck ergonomic load (poor posture, repetitive operations) and neck WMSDs. Improving neck working posture and arranging proper breaks during work may be effective methods for preventing WMSDs by alleviating neck fatigue. FAU - Yang, F AU - Yang F AD - Department of Occupational Health and Poison Control, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200051, China. FAU - Guo, X W AU - Guo XW AD - Laboratory of Occupational Protection and Ergonomics, National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. FAU - Wang, Z X AU - Wang ZX AD - Laboratory of Occupational Protection and Ergonomics, National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. FAU - Jia, N AU - Jia N AD - Laboratory of Occupational Protection and Ergonomics, National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. FAU - Yin, Y AU - Yin Y AD - Department of Occupational Health and Poison Control, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200051, China. LA - chi GR - 2022YFC2503205/The National Key R&D Program of China/ GR - 102393220020090000020/The Project of Occupational Health Risk Assessment and the National Occupational Health Standard Formulation of the National Institute of Occupational Health and Poison Control/ GR - GWVI-11.1-37/The Key Discipline Construction Project of Shanghai Three-Year Action Plan for Strengthening the Public Health System (2023-2025)/ PT - English Abstract PT - Journal Article PL - China TA - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi JT - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases JID - 8410840 SB - IM MH - Humans MH - *Musculoskeletal Diseases/epidemiology MH - *Occupational Diseases/epidemiology MH - *Neck MH - Working Conditions MH - *Ergonomics MH - China/epidemiology MH - Surveys and Questionnaires MH - *Fatigue MH - Adult MH - Male MH - Workload MH - Posture OTO - NOTNLM OT - Ergonomic factors OT - Fatigue OT - Mediating effect OT - Neck OT - Work-related musculoskeletal disorders COIS- 所有作者均声明不存在利益冲突 EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 21:28 PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 21:28 [entrez] AID - 10.3760/cma.j.cn121094-20250815-00332 [doi] PST - ppublish SO - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2026 Oct 20;44(10):793-798. doi: 10.3760/cma.j.cn121094-20250815-00332. PMID- 42957320 OWN - NLM STAT- Publisher LR - 20261029 IS - 1529-2401 (Electronic) IS - 0270-6474 (Linking) DP - 2026 Oct 29 TI - State-Dependent Modulation of Corticospinal and Cortical Responses by Closed-Loop Paired-pulse TMS. LID - e0762262026 [pii] LID - 10.1523/JNEUROSCI.0762-26.2026 [doi] AB - Brain oscillations shape cortical excitability in a phase-dependent manner. Cortical excitability reflects the net output of intracortical circuits, including inhibitory and facilitatory pathways. However, whether the phase of ongoing brain rhythms differentially modulates inhibitory and facilitatory responses remains unknown. Paired-pulse transcranial magnetic stimulation (ppTMS) can selectively engage these circuits, providing a unique tool to dissociate their contributions. We combined real-time EEG-guided closed-loop TMS with single- and paired-pulse TMS in 24 healthy adults (13 female) to probe how sensorimotor mu (8-13 Hz) and beta (14-30 Hz) oscillatory phase shapes responses associated with intracortical inhibitory and facilitatory processes. Single-pulse TMS (spTMS), short-interval intracortical inhibition (SICI), and intracortical facilitation (ICF) were delivered at four phases (peak, falling, trough, rising). Motor evoked potentials (MEPs) and TMS-evoked potentials (TEPs) were recorded to assess corticospinal and cortical responses, respectively. We found that MEP amplitude was phase-dependent across all three stimulation types, with spTMS-evoked and ICF-conditioned MEPs showing the largest amplitudes at the rising phase, whereas SICI-conditioned MEPs were largest at the peak phase of the mu oscillation. TEP P60-N100 amplitude was modulated by oscillatory phase across all stimulation types, with maximal responses at the trough phase. SICI showed opposing phase patterns between the two measures, revealing a dissociation between cortical and corticospinal responses. MEP and TEP preferred phases were correlated across participants only in the ICF condition. These findings extend phase-dependent spTMS effects to paired-pulse stimulation and show that ongoing mu phase differentially shapes responses to SICI and ICF across cortical and corticospinal levels.Significance Statement Ongoing brain oscillations are known to shape cortical excitability across their phase cycle. However, whether this effect differentially influences inhibitory and facilitatory responses has remained unresolved. Here, using closed-loop EEG-guided transcranial magnetic stimulation in healthy adults, we probed how SICI- and ICF-conditioned responses vary across different phases of the sensorimotor mu and beta rhythms. We show that corticospinal and cortical responses to inhibitory and facilitatory paired-pulse protocols exhibit distinct phase-dependent patterns in the sensorimotor mu rhythm. These findings extend phase-dependent stimulation effects beyond single-pulse TMS and reveal that the relationship between cortical state and stimulation response differs across paired-pulse protocols. CI - Copyright (c) 2026 the authors. FAU - Choi, Da Som AU - Choi DS AD - Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA. FAU - Guth, Malte R AU - Guth MR AD - Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA. FAU - Ma, Michelle AU - Ma M AD - Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA. FAU - Harmon, Makaha AU - Harmon M AD - Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA. FAU - Wischnewski, Miles AU - Wischnewski M AD - Department of Experimental Psychology, University of Groningen, Groningen 9712TS, Netherlands. FAU - Park, Jimin AU - Park J AD - Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA. FAU - Blenker, Aneka AU - Blenker A AD - Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA. FAU - Lee, Sangjun AU - Lee S AD - Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA. FAU - Opitz, Alexander AU - Opitz A AD - Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA aopitz@umn.edu. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Neurosci JT - The Journal of neuroscience : the official journal of the Society for Neuroscience JID - 8102140 SB - IM EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 21:13 PHST- 2026/05/05 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 21:13 [entrez] AID - JNEUROSCI.0762-26.2026 [pii] AID - 10.1523/JNEUROSCI.0762-26.2026 [doi] PST - aheadofprint SO - J Neurosci. 2026 Oct 29:e0762262026. doi: 10.1523/JNEUROSCI.0762-26.2026. PMID- 42957318 OWN - NLM STAT- Publisher LR - 20261029 IS - 1529-2401 (Electronic) IS - 0270-6474 (Linking) DP - 2026 Oct 29 TI - PTPRO restricts higher-order branching of terminal arbors through TrkB dephosphorylation. LID - e0713262026 [pii] LID - 10.1523/JNEUROSCI.0713-26.2026 [doi] AB - The structural complexity of axon collateral terminal arbors shapes synaptic connectivity. In corticospinal projections, individual axon collaterals exhibit distinct patterns of terminal arborization, ranging from sparse local branches to dense, highly complex arbors in target regions such as the pontine nuclei. How this complexity is controlled remains poorly understood. Here, using corticopontine projections in male and female mice, we show that protein tyrosine phosphatase receptor type O (PTPRO) selectively restricts higher-order branching of terminal arbors in the pontine nuclei without affecting collateral number. This function requires the intracellular phosphatase domain of PTPRO but not its extracellular region. We demonstrate that PTPRO dephosphorylates tropomyosin receptor kinase B (TrkB), and that the excessive terminal arborization caused by acute PTPRO loss is rescued by TrkB depletion or phosphatidylinositol 3-kinase (PI3K) inhibition. Together, these findings reveal that PTPRO-mediated TrkB dephosphorylation acts as a molecular brake on corticopontine terminal arbor elaboration.Significance Statement How individual axons regulate terminal branching within target regions is a fundamental question in neural circuit development. This study identifies protein tyrosine phosphatase receptor type O (PTPRO), a receptor-type protein tyrosine phosphatase that negatively regulates signaling through tyrosine phosphorylation, as a selective regulator of higher-order branching in corticopontine terminal arbors. PTPRO dephosphorylates tropomyosin receptor kinase B (TrkB), a neurotrophin receptor tyrosine kinase that promotes neuronal growth and branching, thereby restricting terminal arbor complexity without affecting collateral number from the main axon. Given that terminal arbor architecture determines the synaptic territory of each cortical input, PTPRO-dependent constraint on arbor elaboration may represent a general principle by which projection-specific arbor complexity is established across long-range circuits in the central nervous system. CI - Copyright (c) 2026 the authors. FAU - Li, Jiaxuan AU - Li J AD - Graduate School of Frontier Biosciences, The University of Osaka, Osaka, Japan. AD - Department of Anatomy and Neuroscience, Graduate School of Medicine, The University of Osaka, Osaka, Japan. FAU - Yasumura, Misato AU - Yasumura M AD - Department of Anatomy and Neuroscience, Graduate School of Medicine, The University of Osaka, Osaka, Japan. AD - Division of Radioisotope and Radiation Research, Life Science Research Center, University of Toyama, Toyama, Japan. FAU - Iguchi, Tokuichi AU - Iguchi T AD - Division of Developmental Neuroscience, Department of Child Mental Development, United Graduate School of Child Development (UGSCD), The University of Osaka, Osaka, Japan. AD - Laboratory of Anatomy and Physiology, Graduate School of Health Sciences, Fukui Health Science University, Fukui, Japan. AD - Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, Fukui, Japan. FAU - Taniguchi, Manabu AU - Taniguchi M AD - Department of Anatomy and Neuroscience, Graduate School of Medicine, The University of Osaka, Osaka, Japan. FAU - Oka, Yuichiro AU - Oka Y AD - Department of Anatomy and Neuroscience, Graduate School of Medicine, The University of Osaka, Osaka, Japan. AD - Division of Developmental Neuroscience, Department of Child Mental Development, United Graduate School of Child Development (UGSCD), The University of Osaka, Osaka, Japan. FAU - Tsai, Chao-Yuan AU - Tsai CY AD - Department of Anatomy and Neuroscience, Graduate School of Medicine, The University of Osaka, Osaka, Japan. FAU - Sato, Makoto AU - Sato M AD - Graduate School of Frontier Biosciences, The University of Osaka, Osaka, Japan sato.makoto.med@osaka-u.ac.jp. AD - Department of Anatomy and Neuroscience, Graduate School of Medicine, The University of Osaka, Osaka, Japan. AD - Division of Developmental Neuroscience, Department of Child Mental Development, United Graduate School of Child Development (UGSCD), The University of Osaka, Osaka, Japan. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Neurosci JT - The Journal of neuroscience : the official journal of the Society for Neuroscience JID - 8102140 SB - IM EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 21:13 PHST- 2026/04/29 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 21:13 [entrez] AID - JNEUROSCI.0713-26.2026 [pii] AID - 10.1523/JNEUROSCI.0713-26.2026 [doi] PST - aheadofprint SO - J Neurosci. 2026 Oct 29:e0713262026. doi: 10.1523/JNEUROSCI.0713-26.2026. PMID- 42957317 OWN - NLM STAT- Publisher LR - 20261029 IS - 1529-2401 (Electronic) IS - 0270-6474 (Linking) DP - 2026 Oct 29 TI - Hexagonal modulation of theta rhythmic attentional sampling of visual space. LID - e0623252026 [pii] LID - 10.1523/JNEUROSCI.0623-25.2026 [doi] AB - Spatial attention improves visual perception by selecting behaviorally relevant sensory signals. Traditionally, attention has been conceptualized as a static spotlight, while recent evidence posited that attention operates as a moving spotlight that samples visual space sequentially in discrete snapshots that are clocked by theta rhythms ( approximately 3-8 Hz). While theta rhythmic attentional sampling has mainly been observed in fronto-parietal and occipital areas, theta oscillations also hallmark entorhinal-hippocampal grid-cell networks, which encode physical space in hexagonal patterns that guide overt exploration and navigation. We hypothesized that visual attention might rely on the same underlying principles and sample visual space in a hexagonal, grid-like configuration. To test this hypothesis, twenty participants (10 females and 10 males) performed a cue-guided attention task that probed behavioral performance as a function of space and time. Reaction times were assessed as a function of spatial location and varying cue-target intervals, which displayed spatially-structured theta rhythms. Specifically, higher theta power occurred at spatial locations that were aligned to multiples of 60 degrees , consistent with an underlying hexagonal organization. Participants that exhibited stronger hexagonal sampling relied less on the spatial cue to guide their attentional allocation. In sum, these findings suggest that covert visual attention is organized in a hexagonal pattern that might be consistent with the grid-like structure known from the entorhinal-hippocampal system and highlight that theta rhythms could reflect a common organizing principle for spatial cognition.Significance Statement Attention prioritizes sensory inputs to optimize behavior. But how does attention sample the environment in space and time? Here, we demonstrate that attentional sampling of visual space is not uniform, but might preferentially explore locations that are oriented along a hexagonal pattern, reminiscent of the spatial configuration of entorhinal-hippocampal grid cells. Moreover, covert attentional sampling exhibited oscillatory theta signatures (3-8 Hz). In sum, these findings suggest that spatial attention and navigation may share a common organizing principle, and indicate that theta rhythms may orchestrate sampling behaviors in space and time as a unifying principle underlying spatial cognition. CI - Copyright (c) 2026 the authors. FAU - Kienitz, Ricardo AU - Kienitz R AD - Department of Epileptology, University Hospital Bonn, University of Bonn, Venusberg-Campus 1, 53127 Bonn, Germany ricardo.kienitz@gmail.com randolph.helfrich@gmail.com. AD - Goethe University Frankfurt, Epilepsy Center Frankfurt Rhine-Main, Department of Neurology, University Medicine Frankfurt, Schleusenweg 2-16, 60528 Frankfurt am Main, Germany. FAU - Moroz, Michael AU - Moroz M AD - Dept. of Psychology, Yale University, New Haven, CT 06510, USA. AD - Wu Tsai Institute, Yale University, New Haven, CT 06510, USA. FAU - Martini, Jan AU - Martini J AD - Hertie Institute for Clinical Brain Research, Center for Neurology, University Medical Center Tubingen, Tubingen, Germany. AD - International Max Planck Research School for the Mechanisms of Mental Function and Dysfunction, University of Tubingen, Tubingen, Germany. FAU - Helfrich, Randolph F AU - Helfrich RF AD - Dept. of Psychology, Yale University, New Haven, CT 06510, USA. ricardo.kienitz@gmail.com randolph.helfrich@gmail.com. AD - Wu Tsai Institute, Yale University, New Haven, CT 06510, USA. AD - Interdisciplinary Neuroscience Program, Yale University, New Haven, CT 06510, USA. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Neurosci JT - The Journal of neuroscience : the official journal of the Society for Neuroscience JID - 8102140 SB - IM EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 21:13 PHST- 2025/03/28 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 21:13 [entrez] AID - JNEUROSCI.0623-25.2026 [pii] AID - 10.1523/JNEUROSCI.0623-25.2026 [doi] PST - aheadofprint SO - J Neurosci. 2026 Oct 29:e0623252026. doi: 10.1523/JNEUROSCI.0623-25.2026. PMID- 42957299 OWN - NLM STAT- In-Process LR - 20261029 IS - 2044-6055 (Electronic) IS - 2044-6055 (Linking) VI - 16 IP - 10 DP - 2026 Oct 29 TI - Sequential intravesical mitomycin-C and gemcitabine therapy for BCG-unresponsive high-risk non-muscle-invasive bladder cancer: protocol for a prospective, open-label, single-arm, multicentre phase II (IMGeS) study. PG - e117239 LID - 10.1136/bmjopen-2026-117239 [doi] FAU - Lee, Hye Won AU - Lee HW AUID- ORCID: 0000-0002-0303-4658 AD - Department of Urology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea (the Republic of). FAU - Park, Yong Hyun AU - Park YH AUID- ORCID: 0000-0003-4860-6127 AD - Department of Urology, Seoul St Mary's Hospital, The Catholic University of Korea, Seoul, Korea (the Republic of). FAU - Kim, Kyung Hwan AU - Kim KH AD - Department of Urology, Pusan National University Hospital, College of Medicine, Pusan National University, Busan, Korea (the Republic of). FAU - Jung, Eui Hyun AU - Jung EH AUID- ORCID: 0009-0001-6704-0221 AD - Department of Urology, National Cancer Center, Goyang, Korea (the Republic of). FAU - Song, Geehyun AU - Song G AD - Department of Urology, National Cancer Center, Goyang, Korea (the Republic of). FAU - Lee, Hyung Ho AU - Lee HH AD - Department of Urology, National Cancer Center, Goyang, Korea (the Republic of). FAU - Lee, Dong Eun AU - Lee DE AD - Biostatistics Collaboration Team, Research Institute, National Cancer Center, Goyang, Korea (the Republic of). FAU - Kang, Seok Ho AU - Kang SH AUID- ORCID: 0000-0002-1524-5233 AD - Department of Urology, Korea University Hospital, Seoul, Korea (the Republic of). FAU - Jeong, Byung Chang AU - Jeong BC AD - Department of Urology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea (the Republic of). FAU - Ham, Won Sik AU - Ham WS AUID- ORCID: 0000-0003-2246-8838 AD - Department of Urology, Urological Science Institute, Yonsei University College of Medicine, Seoul, Korea (the Republic of). FAU - Jung, Seung Il AU - Jung SI AUID- ORCID: 0000-0003-4864-8175 AD - Department of Urology, Chonnam National University Medical School, Hwasun, Korea (the Republic of). FAU - Oh, Jong Jin AU - Oh JJ AD - Department of Urology, Seoul National University College of Medicine, Seoul National University Bundang Hospital, Seongnam, Korea (the Republic of). FAU - Seo, Ho Kyung AU - Seo HK AUID- ORCID: 0000-0003-2601-1093 AD - Department of Urology, National Cancer Center, Goyang, Korea (the Republic of) seohk@ncc.re.kr. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - BMJ Open JT - BMJ open JID - 101552874 SB - IM OTO - NOTNLM OT - Bladder disorders OT - CHEMOTHERAPY OT - Cancer OT - Safety COIS- Competing interests: EHJ, GS, HHL, DEL and HKS are affiliated with the National Cancer Center, one of the funders of this study. These authors contributed as investigators, independently of the institution's role as a funding body. The National Cancer Center, in its capacity as a funder, had no influence on the content of this manuscript or the decision to submit it for publication. The authors declare no other competing interests. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 21:13 PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 21:13 [entrez] AID - bmjopen-2026-117239 [pii] AID - 10.1136/bmjopen-2026-117239 [doi] PST - epublish SO - BMJ Open. 2026 Oct 29;16(10):e117239. doi: 10.1136/bmjopen-2026-117239. PMID- 42957280 OWN - NLM STAT- Publisher LR - 20261029 IS - 1365-2842 (Electronic) IS - 0305-182X (Linking) DP - 2026 Oct 29 TI - Integrated Multilevel Analysis of Surface Electromyography to Analyse Neuromuscular Patterns in Temporomandibular Disorders. LID - 10.1111/joor.70358 [doi] AB - BACKGROUND: Surface electromyography (sEMG) has been proposed as a non-invasive tool to evaluate masticatory function in patients with temporomandibular disorders (TMD). However, functional indices remain insufficiently characterised. OBJECTIVE: To compare sEMG indices among myogenous and arthrogenous TMD and healthy subjects and explore their relationships. METHODS: This cross-sectional study included 116 myogenous, 68 arthrogenous and 25 healthy subjects. Surface Muscle Index (SMI), Percentage Overlapping Coefficient of anterior temporalis (POC TA) and of masseter muscles (POC MM), Barycentre (BAR), Torsion (TORS), IMPACT and asymmetry were analysed. Kruskal-Wallis tests were used, followed by Dunn's post hoc tests with Holm correction. Effect sizes were estimated using eta-squared based on the H statistic. Directional and status-based categorical variables were compared using chi-square or Fisher's exact tests. Inter-metric associations were explored using Spearman correlation coefficients and exploratory principal component analysis (PCA). RESULTS: Only SMI and BAR showed significant between-group differences (Holm-corrected p = 0.007 and p = 0.001, respectively), with lower values in the myogenous group. IMPACT showed a borderline association with the group (p = 0.052). Categorical analyses revealed significant between-group differences for POC TA side and torsional predominance and IMPACT status. Correlation analysis showed that SMI was strongly associated with BAR (r = 0.83) and TORS (r = 0.74), suggesting a functional organisation. CONCLUSIONS: SMI and BAR were informative for characterising group-level functional differences, particularly those involving the myogenous group. Selected categorical functional patterns, particularly torsional and IMPACT status, differed across groups. These findings support the potential of sEMG metrics in functional characterisation of TMD phenotypes, particularly in myogenous TMD. CI - (c) 2026 John Wiley & Sons Ltd. FAU - Valenti, Chiara AU - Valenti C AUID- ORCID: 0000-0002-7728-9135 AD - CISAS "Giuseppe Colombo" (University Centre for Space Studies and Activities), University of Padua, Padua, Veneto, Italy. AD - Department of Medicine and Surgery, Faculty of Dentistry, University of Perugia, Perugia, Umbria, Italy. FAU - Rahbar, Amirhossein AU - Rahbar A AD - Department of Medicine and Surgery, Faculty of Dentistry, University of Perugia, Perugia, Umbria, Italy. FAU - Betti, Paolo AU - Betti P AUID- ORCID: 0009-0000-0555-0237 AD - Department of Medicine and Surgery, Faculty of Dentistry, University of Perugia, Perugia, Umbria, Italy. FAU - Carli, Elisabetta AU - Carli E AD - Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, Pisa, Tuscany, Italy. FAU - Eramo, Stefano AU - Eramo S AUID- ORCID: 0000-0002-3329-9200 AD - Department of Medicine and Surgery, Faculty of Dentistry, University of Perugia, Perugia, Umbria, Italy. FAU - Pagano, Stefano AU - Pagano S AD - Department of Medicine and Surgery, Faculty of Dentistry, University of Perugia, Perugia, Umbria, Italy. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - J Oral Rehabil JT - Journal of oral rehabilitation JID - 0433604 SB - IM OTO - NOTNLM OT - arthrogenous TMD OT - functional indices OT - myogenous TMD OT - neuromuscular coordination OT - surface electromyography OT - temporomandibular disorders EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 21:02 PHST- 2026/08/28 00:00 [revised] PHST- 2026/05/19 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 21:02 [entrez] AID - 10.1111/joor.70358 [doi] PST - aheadofprint SO - J Oral Rehabil. 2026 Oct 29. doi: 10.1111/joor.70358. PMID- 42957271 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1791-7549 (Electronic) IS - 0258-851X (Linking) VI - 40 IP - 6 DP - 2026 Nov-Dec TI - Significance of Plasma Pro-collagen Type I alpha1 Chain in Patients With Colorectal Cancer. PG - 3630-3639 LID - 10.21873/invivo.14501 [doi] AB - BACKGROUND/AIM: Expression of the gene encoding collagen type I alpha1 chain (COL1A1) in cancer tissue primarily results in production of the pro-form of COL1A1, which can be released into the bloodstream. This study aimed to investigate whether plasma levels of pro-COL1A1 are associated with clinicopathological characteristics and long-term survival in patients with colorectal cancer (CRC). MATERIALS AND METHODS: Plasma levels of pro-pro-COL1A1 were measured in 148 patients with CRC and 28 healthy controls using an enzyme-linked immunosorbent assay (ELISA). RESULTS: Plasma levels of pro-COL1A1 were significantly lower in patients with CRC compared with healthy controls (p<0.001). Lower levels were also observed in men compared with women. Kaplan-Meier survival analysis demonstrated that a low plasma level of pro-COL1A1 was associated with poorer overall survival considering the entire cohort, as well as in subgroups of patients with colon cancer, depth of tumor invasion T3+T4, node-negative (N0) disease and in females. CONCLUSION: These findings suggest that plasma levels of pro-COL1A1 may serve as a prognostic biomarker for long-term survival in patients with CRC. CI - Copyright (c) 2026, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Dimberg, Jan AU - Dimberg J AD - Department of Clinical Diagnostics, School of Health and Welfare, Jonkoping University, Jonkoping, Sweden. FAU - Shamoun, Levar AU - Shamoun L AD - Department of Laboratory Medicine and Pathology, Ryhov Hospital, Jonkoping, Sweden; Levar.Shamoun@rjl.se. FAU - Truong, Son Dinh An AU - Truong SDA AD - Department of Laboratory, Vinmec Danang International General Hospital, Danang, Vietnam. FAU - Landerholm, Kalle AU - Landerholm K AD - Department of Biomedical and Clinical Sciences, Linkoping University, Linkoping, Sweden. AD - Clinical Department of Surgery in Linkoping, Region Ostergotland, Sweden. FAU - Druvefors, Emma AU - Druvefors E AD - Department of Biomedical and Clinical Sciences, Linkoping University, Linkoping, Sweden. AD - Department of Surgery, Ryhov Hospital, Region Jonkoping County, Jonkoping, Sweden. FAU - Wagsater, Dick AU - Wagsater D AD - Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden. LA - eng PT - Journal Article PL - Greece TA - In Vivo JT - In vivo (Athens, Greece) JID - 8806809 RN - 0 (Collagen Type I) RN - 0 (Biomarkers, Tumor) RN - 0 (Collagen Type I, alpha 1 Chain) RN - 0 (COL1A1 protein, human) SB - IM MH - Humans MH - *Colorectal Neoplasms/blood/mortality/pathology/genetics MH - Female MH - *Collagen Type I/blood MH - Male MH - *Biomarkers, Tumor/blood MH - Prognosis MH - Collagen Type I, alpha 1 Chain MH - Aged MH - Middle Aged MH - Kaplan-Meier Estimate MH - Neoplasm Staging MH - Adult OTO - NOTNLM OT - COL1A1 OT - Collagen type I alpha1 chain OT - biomarker OT - clinical parameters OT - colorectal cancer OT - prognostic factor EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 20:53 PHST- 2026/05/29 00:00 [received] PHST- 2026/08/11 00:00 [revised] PHST- 2026/08/13 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - 40/6/3630 [pii] AID - 10.21873/invivo.14501 [doi] PST - ppublish SO - In Vivo. 2026 Nov-Dec;40(6):3630-3639. doi: 10.21873/invivo.14501. PMID- 42957266 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1791-7549 (Electronic) IS - 0258-851X (Linking) VI - 40 IP - 6 DP - 2026 Nov-Dec TI - Uterine Carcinosarcoma With an Adenosquamous Carcinoma and Low-Grade Endometrial Stromal Sarcoma-like Component: A Case Report. PG - 3956-3969 LID - 10.21873/invivo.14533 [doi] AB - BACKGROUND/AIM: Uterine carcinosarcoma, also known as malignant mixed Mullerian tumor, is currently regarded as a metaplastic carcinoma. The sarcomatous component usually consists of homologous or heterologous high-grade mesenchymal elements, whereas low-grade endometrial stromal sarcoma (LG-ESS) has not been recognized as a conventional component of uterine carcinosarcoma. This is a case report of an exceptionally rare biphasic uterine malignancy composed of an adenosquamous carcinoma and an LG-ESS-like stromal component with an extensive morphological transition. CASE REPORT: A 55-year-old nulliparous woman presented with an endometrial mass involving the myometrium and the endocervix. Endometrial biopsy revealed atypical hyperplasia, and cervical punch biopsy showed adenosquamous carcinoma. Subtotal hysterectomy revealed a high-grade endometrioid carcinoma with extensive malignant squamous differentiation (descriptively termed adenosquamous carcinoma in this report). The epithelial component gradually transitioned into diffuse stromal proliferation, which was morphologically consistent with LG-ESS. The stromal component exhibited infiltrative myometrial growth, extensive lymphovascular invasion, diffuse CD10 and vimentin expression, and loss of epithelial markers, whereas the epithelial component showed CK7, PAX8, CEA, and focal estrogen receptor expression. Squamous cell carcinoma, LG-ESS-like stromal component, and transitional area showed block-type p16 positivity and wild-type p53 staining pattern. Postoperatively, the patient received concurrent chemoradiotherapy but experienced rapid disease progression with pelvic recurrence and metastatic lymph node enlargement. Targeted next-generation sequencing revealed microsatellite stability and wild-type BRAF. CONCLUSION: Unlike previously reported synchronous or collision tumors composed of endometrial carcinoma and LG-ESS, this tumor demonstrated a continuous morphological and immunophenotypic spectrum linking the epithelial and stromal components. Although molecular confirmation of clonality was unavailable, the findings favored a single biphasic neoplasm with divergent differentiation rather than two independent tumors. This case expands the currently recognized morphological spectrum of uterine carcinosarcoma and highlights the potential role of epithelial-mesenchymal transition in generating an LG-ESS-like stromal component. CI - Copyright (c) 2026, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Lim, Sung-Chul AU - Lim SC AD - Department of Pathology, College of Medicine, Chosun University, Gwangju, Republic of Korea sclim@chosun.ac.kr. LA - eng PT - Case Reports PT - Journal Article PL - Greece TA - In Vivo JT - In vivo (Athens, Greece) JID - 8806809 RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - Female MH - *Carcinoma, Adenosquamous/pathology/diagnosis MH - *Carcinosarcoma/pathology/diagnosis MH - Middle Aged MH - *Sarcoma, Endometrial Stromal/pathology/diagnosis MH - Biomarkers, Tumor MH - Neoplasm Grading MH - Immunohistochemistry MH - *Uterine Neoplasms/pathology/diagnosis MH - *Endometrial Neoplasms/pathology/diagnosis OTO - NOTNLM OT - Uterine carcinosarcoma OT - adenosquamous carcinoma OT - biphasic uterine neoplasm OT - endometrial carcinoma OT - endometrial stromal sarcoma OT - epithelial-mesenchymal transition OT - histogenesis EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 20:53 PHST- 2026/08/12 00:00 [received] PHST- 2026/09/08 00:00 [revised] PHST- 2026/09/11 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - 40/6/3956 [pii] AID - 10.21873/invivo.14533 [doi] PST - ppublish SO - In Vivo. 2026 Nov-Dec;40(6):3956-3969. doi: 10.21873/invivo.14533. PMID- 42957263 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1791-7549 (Electronic) IS - 0258-851X (Linking) VI - 40 IP - 6 DP - 2026 Nov-Dec TI - Real-time Thermographic Vein Visualization and Extravasation Monitoring During Radiopharmaceutical Injection for Bone Scintigraphy. PG - 3878-3884 LID - 10.21873/invivo.14524 [doi] AB - BACKGROUND/AIM: Prevention of extravasation is important in nuclear medicine, given the growth of radioligand therapy and molecular-targeted radiopharmaceutical therapy. We used very-high-sensitivity infrared thermography to observe the puncture site in real time during radiopharmaceutical injection for bone scintigraphy and evaluated superficial vein visualization and the potential for extravasation monitoring. PATIENTS AND METHODS: A total of 23 patients undergoing bone scintigraphy (19 men, four women; mean age of 74.2+/-7.7 years) were studied. Using very-high-sensitivity infrared thermography (Optris PI 450; thermal resolution 0.04 degrees C), skin-temperature changes around the puncture site were monitored continuously during a saline flush and radiopharmaceutical injection. The minimum skin temperature within 5 mm of the puncture site was compared before and during injection, and its relationship with body mass index and injection site was examined. RESULTS: No extravasation occurred, and bone scintigraphy was completed normally in all 23 patients. Superficial veins were clearly visualized in 19 out of 23 patients (83%): visualization succeeded in all forearm injections (4/4, 100%) but was poor in 4 out of 19 antecubital-fossa injections (21%). Among the 19 patients with venous visualization >/=5 mm, median minimum skin temperature within 5 mm of the puncture site decreased from 33.62 degrees C before injection to 28.79 degrees C during injection, with no temperature change suggestive of subcutaneous extravasation. CONCLUSION: Real-time skin-temperature monitoring with very-high-sensitivity infrared thermography may aid superficial vein visualization and early detection of extravasation. Because visualization is affected by the site of injection, careful site selection and observation are warranted. CI - Copyright (c) 2026, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Kageyama, Masatoshi AU - Kageyama M AD - Long-Term Care Health Facilities Sunrise Ohhama, Shizuoka, Japan. FAU - Konishi, Kenta AU - Konishi K AD - Department of Radiation Oncology, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Ikenohira, Tsutomu AU - Ikenohira T AD - Department of Radiation Oncology, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Wakabayashi, Kohei AU - Wakabayashi K AD - Department of Radiation Oncology, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Yamashita, Rintaro AU - Yamashita R AD - Department of Radiation Oncology, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Tanahashi, Yukichi AU - Tanahashi Y AD - Department of Radiology, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Ozaki, Kumi AU - Ozaki K AD - Department of Radiology, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Goshima, Satoshi AU - Goshima S AD - Department of Radiology, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Nakamura, Katsumasa AU - Nakamura K AD - Department of Radiation Oncology, Hamamatsu University School of Medicine, Hamamatsu, Japan; nakam@hama-med.ac.jp. LA - eng PT - Journal Article PL - Greece TA - In Vivo JT - In vivo (Athens, Greece) JID - 8806809 RN - 0 (Radiopharmaceuticals) SB - IM MH - Humans MH - Female MH - *Thermography/methods MH - Aged MH - Male MH - *Radiopharmaceuticals/administration & dosage MH - *Radionuclide Imaging/methods MH - *Extravasation of Diagnostic and Therapeutic Materials/diagnostic imaging/diagnosis MH - *Bone and Bones/diagnostic imaging MH - *Veins/diagnostic imaging MH - Skin Temperature MH - Middle Aged MH - Aged, 80 and over OTO - NOTNLM OT - Infrared thermography OT - bone scintigraphy OT - extravasation OT - radiopharmaceutical OT - vein visualization EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 20:53 PHST- 2026/08/09 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - 40/6/3878 [pii] AID - 10.21873/invivo.14524 [doi] PST - ppublish SO - In Vivo. 2026 Nov-Dec;40(6):3878-3884. doi: 10.21873/invivo.14524. PMID- 42957262 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1791-7549 (Electronic) IS - 0258-851X (Linking) VI - 40 IP - 6 DP - 2026 Nov-Dec TI - Initial Clinical Experience With Gemcitabine, Cisplatin, and Nivolumab for Advanced Urothelial Carcinoma: A Four-case Series. PG - 3912-3921 LID - 10.21873/invivo.14528 [doi] AB - BACKGROUND/AIM: Gemcitabine, cisplatin, and nivolumab (GCNivo) has become an established first-line treatment for advanced urothelial carcinoma (UC) following the CheckMate 901 trial. However, real-world evidence is limited, particularly regarding shared decision-making in first-line treatment selection and subsequent management after induction therapy. We present four cases illustrating the selection of first-line GCNivo and subsequent therapeutic pathways in routine clinical practice. CASE REPORT: We report four patients with advanced UC who received first-line GCNivo. All patients experienced distinct clinical courses. GCNivo was selected through shared decision-making that considered expected efficacy, toxicity, clinical characteristics, and patient preference. An 83-year-old woman with locally advanced bladder cancer achieved a radiological complete response after four cycles. The patient underwent consolidative bladder radiotherapy followed by observation without nivolumab maintenance because of treatment-related toxicities, advanced age, and patient preference. A 71-year-old man with nodal recurrence after radical cystectomy achieved a sustained partial response and transitioned to nivolumab maintenance after completion of induction GCNivo. A 69-year-old man with rapidly progressive metastatic disease initially responded to GCNivo but subsequently experienced clinical deterioration with suspected disease progression, prompting a switch to second-line enfortumab vedotin. A 75-year-old man with synchronous bladder and upper tract UC completed four induction cycles before transitioning to nivolumab maintenance because of declining renal function despite maintained disease control. In all four cases, management was individualized according to treatment response and tolerability. CONCLUSION: GCNivo produced meaningful antitumor responses with generally manageable toxicities in patients with advanced UC. These cases highlight the importance of shared decision-making when selecting GCNivo among multiple first-line treatment options as well as individualized post-induction management. GCNivo may serve as a therapeutic platform for tailored multidisciplinary management. CI - Copyright (c) 2026, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Tsubota, Shuji AU - Tsubota S AD - Department of Uro-Oncology, Saitama Medical University International Medical Center, Hidaka, Japan. FAU - Kaneko, G O AU - Kaneko GO AD - Department of Uro-Oncology, Saitama Medical University International Medical Center, Hidaka, Japan gk3281@5931.saitama-med.ac.jp. FAU - Igarashi, Daisuke AU - Igarashi D AD - Department of Uro-Oncology, Saitama Medical University International Medical Center, Hidaka, Japan. FAU - Takahashi, Takayuki AU - Takahashi T AD - Department of Uro-Oncology, Saitama Medical University International Medical Center, Hidaka, Japan. FAU - Baba, Yuto AU - Baba Y AD - Department of Uro-Oncology, Saitama Medical University International Medical Center, Hidaka, Japan. FAU - Shirotake, Suguru AU - Shirotake S AD - Department of Uro-Oncology, Saitama Medical University International Medical Center, Hidaka, Japan. LA - eng PT - Case Reports PT - Journal Article PL - Greece TA - In Vivo JT - In vivo (Athens, Greece) JID - 8806809 RN - 0 (Gemcitabine) RN - 31YO63LBSN (Nivolumab) RN - Q20Q21Q62J (Cisplatin) RN - 0W860991D6 (Deoxycytidine) SB - IM MH - Humans MH - Female MH - Gemcitabine MH - Nivolumab/administration & dosage MH - Cisplatin/administration & dosage MH - Aged, 80 and over MH - Deoxycytidine/analogs & derivatives/administration & dosage MH - *Antineoplastic Combined Chemotherapy Protocols/therapeutic use/adverse effects MH - Aged MH - Male MH - Treatment Outcome MH - Neoplasm Staging MH - *Urinary Bladder Neoplasms/drug therapy/pathology MH - *Carcinoma, Transitional Cell/drug therapy/pathology MH - Urothelium/pathology OTO - NOTNLM OT - Urothelial carcinoma OT - case series OT - chemoimmunotherapy OT - cisplatin OT - gemcitabine OT - nivolumab EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 20:53 PHST- 2026/08/20 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - 40/6/3912 [pii] AID - 10.21873/invivo.14528 [doi] PST - ppublish SO - In Vivo. 2026 Nov-Dec;40(6):3912-3921. doi: 10.21873/invivo.14528. PMID- 42957261 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1791-7549 (Electronic) IS - 0258-851X (Linking) VI - 40 IP - 6 DP - 2026 Nov-Dec TI - Definitive Hypofractionated Radiotherapy in Elderly Patients With Non-melanoma Skin Cancer: Predicting Complete Response Using a Machine Learning-based Approach. PG - 3685-3691 LID - 10.21873/invivo.14507 [doi] AB - BACKGROUND/AIM: This study aimed to develop a classification tree model to predict clinical complete response (cCR) after definitive hypofractionated RT in elderly patients with non-melanoma (basal and squamous cell carcinoma) skin cancer based on pre-treatment clinical parameters. PATIENTS AND METHODS: Data from elderly patients (aged >/= 70 years) with histologically proven non-melanoma skin cancer lesion(s) were reviewed. All lesions were treated with definitive hypofractionated RT. A decision tree methodology was applied to define significant pre-treatment parameters influencing the cCR rate. The data set included seven predictor variables (location, histology, T stage, diameter, bleeding, pain, previous treatment) and a dependent variable (cCR). The analysis was conducted using open-source R software. Important explanatory variables were selected using the random forest approach. A classification tree that optimally partitioned patients according to cCR was then built. RESULTS: A total of 121 patients with 145 non-melanoma skin cancer lesions were identified between January 2015 and December 2023. The cCR rate after treatment was 78.6%. The three most important variables identified were T stage, location and pain at diagnosis. Based on these explanatory variables, patients were partitioned in five groups. Of these groups, three experienced cCR rates ranging from 69% to 88% and two experienced cCR rates lower than 15%. CONCLUSION: The proposed classification tree could serve as a valuable tool to guide future research in the field of non-melanoma skin cancer. The decision tree method may be an effective approach to partition elderly patients with non-melanoma skin cancer based on key clinical parameters. CI - Copyright (c) 2026, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - DE Felice, Francesca AU - DE Felice F AD - Department of Radiotherapy, Policlinico Umberto I, Department of Radiological, Oncological and Pathological Sciences, "Sapienza" University of Rome, Rome, Italy; fradefelice@hotmail.it. FAU - Spinelli, Lavinia AU - Spinelli L AD - Radiotherapy Department S.Croce & Carle Teaching Hospital, Cuneo, Italy. FAU - Critelli, Paola AU - Critelli P AD - Radiotherapy Department S.Croce & Carle Teaching Hospital, Cuneo, Italy. FAU - Scricciolo, Melissa AU - Scricciolo M AD - Department of Radiation Oncology, Ospedale dell'Angelo, Venice, Italy. FAU - Musio, Daniela AU - Musio D AD - Department of Radiotherapy Santa Maria Addolorata Hospital, Rome, Italy. FAU - Magnante, Annalisa AU - Magnante A AD - Department of Radiotherapy, Policlinico Umberto I, Department of Radiological, Oncological and Pathological Sciences, "Sapienza" University of Rome, Rome, Italy. FAU - Martini, Stefania AU - Martini S AD - Radiotherapy Department S.Croce & Carle Teaching Hospital, Cuneo, Italy. FAU - Minniti, Giuseppe AU - Minniti G AD - Department of Radiotherapy, Policlinico Umberto I, Department of Radiological, Oncological and Pathological Sciences, "Sapienza" University of Rome, Rome, Italy. FAU - Merlotti, Anna AU - Merlotti A AD - Department of Radiation Oncology, Ospedale dell'Angelo, Venice, Italy. LA - eng PT - Journal Article PL - Greece TA - In Vivo JT - In vivo (Athens, Greece) JID - 8806809 SB - IM MH - Humans MH - Female MH - Male MH - Aged, 80 and over MH - Aged MH - Treatment Outcome MH - *Machine Learning MH - *Radiation Dose Hypofractionation MH - Non-Melanoma Skin Neoplasms/radiotherapy MH - Neoplasm Staging MH - *Skin Neoplasms/radiotherapy MH - Prognosis MH - Cutaneous Squamous Cell Carcinoma MH - Random Forest MH - Classification Algorithms OTO - NOTNLM OT - Elderly OT - cemiplimab OT - complete response OT - non-melanoma skin cancer OT - radiotherapy EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 20:53 PHST- 2026/08/24 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - 40/6/3685 [pii] AID - 10.21873/invivo.14507 [doi] PST - ppublish SO - In Vivo. 2026 Nov-Dec;40(6):3685-3691. doi: 10.21873/invivo.14507. PMID- 42957260 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1791-7549 (Electronic) IS - 0258-851X (Linking) VI - 40 IP - 6 DP - 2026 Nov-Dec TI - CRP-Albumin-Lymphocyte (CALLY) Index Is an Independent Prognostic Factor for Recurrent Esophageal Cancer After Curative Treatment. PG - 3821-3829 LID - 10.21873/invivo.14518 [doi] AB - BACKGROUND/AIM: The C-reactive protein-albumin-lymphocyte (CALLY) index is a promising biomarker. We investigated the prognostic significance of the CALLY index in patients with recurrent esophageal cancer (EC). PATIENTS AND METHODS: Consecutive patients who developed recurrent EC after curative esophagectomy and received treatment for recurrence at Yokohama City University from 2005 to 2022 were selected based on medical records. The CALLY index was calculated as follows: serum albumin level (g/dl) x peripheral lymphocyte count (/mul)/[C-reactive protein level (mg/dl) x 10(4)]. The primary endpoint was overall survival after recurrence. The secondary endpoint was the association between the CALLY index and the clinical course after recurrence, including the administration of first-, second-, and third- line treatments. RESULTS: One hundred patients were included in this retrospective study. The cut-off value for the CALLY index was set at 5. The 5-year overall survival rates were 11.2% and 46.9% for the low and high CALLY index groups, respectively (p<0.001). Multivariate analysis demonstrated that a low CALLY index was an independent prognostic factor for poor overall survival (hazard ratio=4.271, 95% confidence interval=2.370-7.696, p<0.001). Patients with a high CALLY index were significantly more likely to receive first-, second-, and third-line treatments than those with a low CALLY index. CONCLUSION: The CALLY index may serve as a useful prognostic biomarker and may also be associated with the ability to receive subsequent lines of treatment in patients with recurrent esophageal cancer. CI - Copyright (c) 2026, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Takagi, Maki AU - Takagi M AD - Department of Gastrointestinal Surgery, Yokohama City University, Yokohama, Japan; takagi.mak.jz@yokohama-cu.ac.jp. AD - Department of Surgery, Yokohama City University, Yokohama, Japan. FAU - Aoyama, Toru AU - Aoyama T AD - Department of Gastrointestinal Surgery, Yokohama City University, Yokohama, Japan. AD - Department of Surgery, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital, Tokyo, Japan. FAU - Hashimoto, Itaru AU - Hashimoto I AD - Department of Gastrointestinal Surgery, Yokohama City University, Yokohama, Japan. FAU - Kawabe, Taiichi AU - Kawabe T AD - Department of Gastrointestinal Surgery, Yokohama City University, Yokohama, Japan. FAU - Maezawa, Yukio AU - Maezawa Y AD - Department of Gastrointestinal Surgery, Yokohama City University, Yokohama, Japan. FAU - Sato, Sho AU - Sato S AD - Department of Gastrointestinal Surgery, Yokohama City University, Yokohama, Japan. FAU - DEN, Kanechika AU - DEN K AD - Department of Gastrointestinal Surgery, Yokohama City University, Yokohama, Japan. FAU - Kosaka, Takashi AU - Kosaka T AD - Department of Gastrointestinal Surgery, Yokohama City University, Yokohama, Japan. FAU - Sato, Tsutomu AU - Sato T AD - Department of Gastrointestinal Surgery, Yokohama City University, Yokohama, Japan. FAU - Atsumi, Yosuke AU - Atsumi Y AD - Department of Gastrointestinal Surgery, Yokohama City University, Yokohama, Japan. FAU - Kayashima, Miki AU - Kayashima M AD - Department of Gastrointestinal Surgery, Yokohama City University, Yokohama, Japan. FAU - Sekiguchi, Kohei AU - Sekiguchi K AD - Department of Gastrointestinal Surgery, Yokohama City University, Yokohama, Japan. FAU - Tamagawa, Ayako AU - Tamagawa A AD - Department of Gastrointestinal Surgery, Yokohama City University, Yokohama, Japan. FAU - Kazama, Keisuke AU - Kazama K AD - Department of Gastrointestinal Surgery, Yokohama City University, Yokohama, Japan. FAU - Sugano, Nobuhiro AU - Sugano N AD - Department of Gastrointestinal Surgery, Yokohama City University, Yokohama, Japan. FAU - Cho, Haruhiko AU - Cho H AD - Department of Gastrointestinal Surgery, Yokohama City University, Yokohama, Japan. AD - Department of Surgery, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital, Tokyo, Japan. FAU - Yukawa, Norio AU - Yukawa N AD - Department of Gastrointestinal Surgery, Yokohama City University, Yokohama, Japan. FAU - Yoshikawa, Takaki AU - Yoshikawa T AD - Department of Gastrointestinal Surgery, Yokohama City University, Yokohama, Japan. LA - eng PT - Journal Article PL - Greece TA - In Vivo JT - In vivo (Athens, Greece) JID - 8806809 RN - 9007-41-4 (C-Reactive Protein) RN - 0 (Biomarkers, Tumor) RN - 0 (Serum Albumin) SB - IM MH - Humans MH - *Esophageal Neoplasms/blood/therapy/pathology/mortality/diagnosis MH - Prognosis MH - Female MH - Male MH - *Neoplasm Recurrence, Local/blood/pathology MH - *C-Reactive Protein/metabolism MH - Aged MH - *Biomarkers, Tumor/blood MH - Middle Aged MH - *Lymphocytes/pathology/metabolism MH - *Serum Albumin MH - Lymphocyte Count MH - Retrospective Studies OTO - NOTNLM OT - CALLY index OT - esophageal cancer OT - overall survival OT - prognosis OT - recurrence EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 20:53 PHST- 2026/07/14 00:00 [received] PHST- 2026/08/25 00:00 [revised] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - 40/6/3821 [pii] AID - 10.21873/invivo.14518 [doi] PST - ppublish SO - In Vivo. 2026 Nov-Dec;40(6):3821-3829. doi: 10.21873/invivo.14518. PMID- 42957252 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1791-7549 (Electronic) IS - 0258-851X (Linking) VI - 40 IP - 6 DP - 2026 Nov-Dec TI - Hepatobiliary Adverse Events Associated With Avacopan: Analysis of the JADER Database. PG - 3716-3724 LID - 10.21873/invivo.14510 [doi] AB - BACKGROUND/AIM: Avacopan, a selective C5a receptor antagonist approved for anti-neutrophil cytoplasmic antibody-associated vasculitis, has been associated with hepatobiliary disorders including vanishing bile duct syndrome (VBDS). These events have been reported more frequently in Japanese patients than in Western populations. To address this population-specific safety concern, we characterized the reporting patterns of avacopan-associated hepatobiliary disorders using the Japanese Adverse Drug Event Report (JADER) database. PATIENTS AND METHODS: We analyzed reports submitted to JADER between April 2004 and January 2026 in which avacopan was registered as a suspect drug. Hepatobiliary adverse events were defined as a composite set comprising preferred terms (PTs) under the System Organ Class (SOC) "Hepatobiliary disorders" (code 10019805) of Medical Dictionary for Regulatory Activities (MedDRA)/J version 29.0 and two liver-related PTs classified under the SOC "Investigations" (code 10022891): Liver function test increased (10077692) and hepatic enzymes increased (10060795). Disproportionality was assessed using the reporting odds ratio with 95% confidence intervals (CIs); a signal was defined as three or more reports with a lower CI limit exceeding 1.0. Time to onset was calculated from treatment start to hepatobiliary adverse events onset, and post-event outcomes were categorized based on available JADER outcome data. RESULTS: Among 685 avacopan-related reports, disproportionality signals were detected for all hepatobiliary adverse events evaluated. The median time to onset of hepatobiliary adverse events ranged from 33 to 56.5 days, with most events occurring within one to two months after avacopan treatment. Of 226 reports with outcome data, 19 (8.4%) recorded death. Notably, 13 of 27 VBDS reports (48.1%) recorded death as the outcome, a markedly higher proportion than that for other hepatobiliary adverse events. CONCLUSION: These findings support the importance of continued post-marketing surveillance and monitoring of hepatobiliary events during avacopan treatment. CI - Copyright (c) 2026, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Fujiwara, Masaki AU - Fujiwara M AD - School of Pharmacy, Hyogo Medical University, Hyogo, Japan. FAU - Kotake, Kazumasa AU - Kotake K AD - Department of Pharmacy, Zikei Hospital/Zikei Institute of Psychiatry, Okayama, Japan kottanketty@gmail.com. FAU - Uemichi, Yuki AU - Uemichi Y AD - School of Pharmacy, Hyogo Medical University, Hyogo, Japan. FAU - Shimizu, Tadashi AU - Shimizu T AD - School of Pharmacy, Hyogo Medical University, Hyogo, Japan. LA - eng PT - Journal Article PL - Greece TA - In Vivo JT - In vivo (Athens, Greece) JID - 8806809 SB - IM MH - Humans MH - Adverse Drug Reaction Reporting Systems MH - Databases, Factual MH - Female MH - Male MH - *Drug-Related Side Effects and Adverse Reactions/epidemiology/etiology MH - *Liver Diseases/epidemiology/etiology MH - *Chemical and Drug Induced Liver Injury/epidemiology/etiology MH - Japan/epidemiology MH - Middle Aged MH - Aged OTO - NOTNLM OT - ANCA-associated vasculitis OT - Avacopan OT - JADER OT - Japan OT - hepatobiliary disorders OT - pharmacovigilance EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 20:53 PHST- 2026/06/01 00:00 [received] PHST- 2026/07/23 00:00 [revised] PHST- 2026/08/12 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - 40/6/3716 [pii] AID - 10.21873/invivo.14510 [doi] PST - ppublish SO - In Vivo. 2026 Nov-Dec;40(6):3716-3724. doi: 10.21873/invivo.14510. PMID- 42957244 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1791-7549 (Electronic) IS - 0258-851X (Linking) VI - 40 IP - 6 DP - 2026 Nov-Dec TI - Prostate-directed Radiotherapy in Selected Patients With Metastatic Castration-sensitive Prostate Cancer: A Real-world Case Series. PG - 3843-3853 LID - 10.21873/invivo.14520 [doi] AB - BACKGROUND/AIM: Prostate-directed radiotherapy (RT) improves outcomes in selected patients with metastatic castration-sensitive prostate cancer (mCSPC), particularly those with low metastatic burden. However, real-world information regarding its use after response to systemic therapy remains limited. This study describes the treatment characteristics, safety, and clinical course of selected patients with mCSPC who received prostate-directed RT. PATIENTS AND METHODS: We retrospectively reviewed an institutional RT database containing 396 patients who initiated prostate-directed RT between April 2015 and March 2026. Thirteen patients had mCSPC at RT initiation and were included. Prostate-directed RT was considered after a marked prostate-specific antigen (PSA) decline and confirmation of no radiographic progression during first-line systemic therapy; no prespecified PSA threshold was used. Patient characteristics, RT details, RT-related adverse events, and clinical outcomes after RT completion were evaluated. RESULTS: Eight patients had low-volume and five had high-volume disease. The median interval from initiation of first-line systemic therapy to RT was 8.4 months. The median PSA decline before RT was 99.9% (interquartile range=98.9-99.9%), and the median PSA level before RT was 0.028 ng/ml. All patients received intensity-modulated RT, and residual metastatic lesions were included in the RT target volume in six patients. All patients completed RT. No grade >/=2 acute genitourinary or gastrointestinal toxicity was observed, and no late RT-related toxicity occurred among the 11 patients evaluable for late toxicity. During follow-up (median, 39.6 months) after RT, four patients developed castration-resistant disease and four experienced radiographic progression. No post-RT local events or prostate cancer-specific deaths occurred; one patient died of an unrelated cause. CONCLUSION: In this small, highly selected cohort of patients demonstrating favorable initial responses to systemic therapy, prostate-directed RT was feasible and well tolerated, with no grade >/=2 acute RT-related toxicity, no late toxicity among evaluable patients, and no local events. Comparative efficacy cannot be determined because of the retrospective design, small sample size, and absence of a non-RT control group. CI - Copyright (c) 2026, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Furubayashi, Nobuki AU - Furubayashi N AD - Department of Urology, National Hospital Organization Kyushu Cancer Center, Fukuoka, Japan; nobumduro@gmail.com. FAU - Nakashima, Takaaki AU - Nakashima T AD - Department of Radiation Oncology, National Hospital Organization Kyushu Cancer Center, Fukuoka, Japan. FAU - Uehara, Ryuji AU - Uehara R AD - Department of Radiation Oncology, National Hospital Organization Kyushu Cancer Center, Fukuoka, Japan. FAU - Kojima, Takehito AU - Kojima T AD - Department of Urology, National Hospital Organization Kyushu Cancer Center, Fukuoka, Japan. FAU - Nakano, Yasuhiro AU - Nakano Y AD - Department of Urology, National Hospital Organization Kyushu Cancer Center, Fukuoka, Japan. FAU - Kataoka, Akari AU - Kataoka A AD - Department of Urology, National Hospital Organization Kyushu Cancer Center, Fukuoka, Japan. FAU - Abe, Madoka AU - Abe M AD - Department of Radiation Oncology, National Hospital Organization Kyushu Cancer Center, Fukuoka, Japan. FAU - Negishi, Takahito AU - Negishi T AD - Department of Urology, National Hospital Organization Kyushu Cancer Center, Fukuoka, Japan. LA - eng PT - Journal Article PL - Greece TA - In Vivo JT - In vivo (Athens, Greece) JID - 8806809 RN - EC 3.4.21.77 (Prostate-Specific Antigen) SB - IM MH - Humans MH - Male MH - Aged MH - Treatment Outcome MH - Prostate-Specific Antigen/blood MH - Neoplasm Metastasis MH - *Prostatic Neoplasms, Castration-Resistant/radiotherapy/pathology MH - Middle Aged MH - Retrospective Studies MH - Aged, 80 and over OTO - NOTNLM OT - Metastatic castration-sensitive prostate cancer OT - local therapy OT - prostate-directed radiotherapy OT - real-world evidence OT - toxicity EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 20:53 PHST- 2026/07/31 00:00 [received] PHST- 2026/09/02 00:00 [revised] PHST- 2026/09/03 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - 40/6/3843 [pii] AID - 10.21873/invivo.14520 [doi] PST - ppublish SO - In Vivo. 2026 Nov-Dec;40(6):3843-3853. doi: 10.21873/invivo.14520. PMID- 42957243 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1791-7549 (Electronic) IS - 0258-851X (Linking) VI - 40 IP - 6 DP - 2026 Nov-Dec TI - Differential Modulation of Galectin-3 by Tamoxifen and Progesterone Receptor Modulators: An In Vitro and In Silico Study. PG - 3429-3444 LID - 10.21873/invivo.14487 [doi] AB - BACKGROUND/AIM: Tamoxifen remains one of the most widely used endocrine treatments for hormone-receptor-positive breast cancer. However, its partial estrogen-agonist activity in the endometrium is associated with endometrial proliferation, hyperplasia, polyps, and, rarely, carcinoma. Antiprogestins and selective progesterone receptor modulators, including mifepristone and ulipristal acetate, have been proposed as alternative or adjunctive endocrine strategies, but their effects on endometrial molecular remodeling remain insufficiently characterized. This study investigates whether Galectin-3, a multifunctional lectin that is involved in epithelial integrity, angiogenesis, receptor signaling, and tumor-associated remodeling, could serve as a candidate marker of the endometrial epithelial response to endocrine modulation. MATERIALS AND METHODS: Ishikawa endometrial adenocarcinoma cells were exposed to different concentrations of tamoxifen, mifepristone, and ulipristal acetate. Galectin-3 protein expression was evaluated using Western blot analysis, and densitometric measurements were normalized to beta-actin. Complementary STRING and STITCH bioinformatics analyses were performed to examine the molecular interactions linking Galectin-3 with steroid receptor and growth-factor-associated signaling pathways. RESULTS: Tamoxifen produced a biphasic response, characterized by modest induction of Galectin-3 expression at low concentration and marked suppression at intermediate and high concentrations. Mifepristone preserved Galectin-3 expression at low and intermediate concentrations but reduced its expression at the highest concentration. Ulipristal acetate increased Galectin-3 expression at low-to-intermediate concentrations, followed by a return toward baseline at high concentration. STRING and STITCH analyses indicated that Galectin-3 is indirectly linked to steroid receptor signaling through growth-factor-associated pathways, particularly EGFR/ERBB- and VEGF-related networks. CONCLUSION: Galectin-3 expression differs according to the type and concentration of endocrine modulator used, suggesting that it may reflect distinct endometrial epithelial responses to hormonal treatment. Galectin-3 therefore warrants further investigation as a potential biomarker for assessing endometrial safety during endocrine therapy. CI - Copyright (c) 2026, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Kontogeorgi, Adamantia AU - Kontogeorgi A AD - Medical School, University of Crete, Heraklion, Greece; Adamantia.kondogewrgi@gmail.com. FAU - Boutas, Ioannis AU - Boutas I AD - Department of Obstetrics and Gynecology, University of Crete, Heraklion, Greece. FAU - Agrogiannis, George AU - Agrogiannis G AD - 2nd Department of Propedeutic Surgery, "Laiko" General Hospital, National and Kapodistrian University of Athens, Medical School, Athens, Greece. FAU - Kalaitzis, Dimitrios AU - Kalaitzis D AD - Independent Authority of Public Revenue, Athens, Greece. FAU - Driva, Tatiana S AU - Driva TS AD - 1st Department of Pathology, General Hospital of Athens "Laiko", Medical School, National and Kapodistrian University of Athens, Athens, Greece. FAU - Zoumakis, Emmanouil AU - Zoumakis E AD - 1st Department of Pediatrics, University of Athens Medical School, Agia Sofia Hospital, Athens, Greece. FAU - Kalantaridou, Sophia N AU - Kalantaridou SN AD - Medical School, National and Kapodistrian University of Athens, Athens, Greece. FAU - Makrigiannakis, Antonios AU - Makrigiannakis A AD - Department of Obstetrics and Gynecology, University of Crete, Heraklion, Greece. LA - eng PT - Journal Article PL - Greece TA - In Vivo JT - In vivo (Athens, Greece) JID - 8806809 RN - 094ZI81Y45 (Tamoxifen) RN - 0 (Receptors, Progesterone) RN - 0 (Galectin 3) RN - 0 (Norpregnadienes) RN - 320T6RNW1F (Mifepristone) RN - 0 (Galectins) RN - YF7V70N02B (ulipristal acetate) RN - 0 (Blood Proteins) RN - 0 (LGALS3 protein, human) SB - IM MH - Humans MH - Female MH - *Tamoxifen/pharmacology MH - *Receptors, Progesterone/metabolism/antagonists & inhibitors MH - *Galectin 3/metabolism/genetics MH - Signal Transduction/drug effects MH - Norpregnadienes/pharmacology MH - Mifepristone/pharmacology MH - Cell Line, Tumor MH - Galectins MH - Endometrium/drug effects/metabolism/pathology MH - Endometrial Neoplasms/drug therapy/metabolism/pathology/genetics MH - Blood Proteins OTO - NOTNLM OT - Galectin-3 OT - antiprogestins OT - endocrine therapy OT - endometrial safety EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 20:53 PHST- 2026/08/04 00:00 [received] PHST- 2026/09/10 00:00 [revised] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - 40/6/3429 [pii] AID - 10.21873/invivo.14487 [doi] PST - ppublish SO - In Vivo. 2026 Nov-Dec;40(6):3429-3444. doi: 10.21873/invivo.14487. PMID- 42957242 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1791-7549 (Electronic) IS - 0258-851X (Linking) VI - 40 IP - 6 DP - 2026 Nov-Dec TI - L-Glutamine for Pediatric Sickle Cell Disease: Pharmacology, Mechanism of Action, Safety and Efficacy. PG - 3253-3261 LID - 10.21873/invivo.14475 [doi] AB - BACKGROUND/AIM: L-Glutamine (L-Gln) has been approved for the treatment of sickle cell disease (SCD) since 2017. Clinical and pharmacological data in pediatric patients remain limited. This review summarizes studies of L-Gln use in SCD including pharmacokinetics (PK), pharmacodynamics (PD), safety, and efficacy in pediatrics, alongside in-vitro and pre-clinical data elucidating primary and supplemental mechanisms of action (MOA). Pharmacoeconomics (PE) is addressed briefly. MATERIALS AND METHODS: Literature search included keywords of SCD, L-Gln, and pediatrics - targeting papers with PK, PD, PE, clinical and preclinical studies. Thirty-two articles meeting the selection criteria were included for descriptive and analytical review. RESULTS: Two mouse studies were identified. The remaining articles included translational, Phase-2 and Phase-3 trials and studies containing pharmacological data. Pharmacokinetics showed serum L-Gln levels to peak at 800-1000 muM within 1 h after oral intake of clinically relevant doses. Thereafter, approximately 500-600 muM levels were shown to last 4-8 h post-dose. Pharmacodynamic data suggest that antioxidant effects may represent an important mechanism of action mainly via increased NADH, but additional contributors may include increased glutathione, decreased cell adhesion, and up-regulated autophagy of peripheral blood mononuclear cells. Five papers compared L-Gln with other SCD-modifying therapies. Indirect comparisons suggest that L-Gln, hydroxyurea, and crizanlizumab can each reduce vaso-occlusive events with significant decrease of pain crises and hospitalizations. However, direct comparative efficacy cannot be established in the absence of head-to-head trials. Overall safety profile was also comparable; however L-Gln may cause more gastrointestinal (GI) side effects such as constipation and abdominal pain. Annual cost of L-Gln is lower than crizanlizumab, however insurance denials and high co-pays were reported as barriers to initiating the therapy. CONCLUSION: Oral L-Gln is an effective SCD-modifying therapy for patients with sickle cell disease 5 years and older. Gastrointestinal adverse effects, including abdominal pain and constipation, are reported. PK supports twice daily regimen, and PD suggests that anti-oxidative effect is the dominant MOA. Cost reductions may result in wider use of L-Gln. CI - Copyright (c) 2026, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Panosyan, Eduard H AU - Panosyan EH AD - Division of Pediatric Hematology-Oncology, Department of Pediatrics, Harbor-UCLA Medical Center, Torrance, CA, U.S.A.; epanosyan@lundquist.org. AD - The Lundquist Institute for Biomedical Innovation, Torrance, CA, U.S.A. LA - eng PT - Journal Article PT - Review PL - Greece TA - In Vivo JT - In vivo (Athens, Greece) JID - 8806809 RN - 0RH81L854J (Glutamine) SB - IM MH - Humans MH - *Anemia, Sickle Cell/drug therapy/metabolism MH - *Glutamine/therapeutic use/pharmacokinetics/pharmacology/adverse effects/administration & dosage MH - Animals MH - Child MH - Treatment Outcome OTO - NOTNLM OT - Glutamine OT - pharmacology OT - review OT - sickle cell disease EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 20:53 PHST- 2026/08/07 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - 40/6/3253 [pii] AID - 10.21873/invivo.14475 [doi] PST - ppublish SO - In Vivo. 2026 Nov-Dec;40(6):3253-3261. doi: 10.21873/invivo.14475. PMID- 42957240 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1791-7549 (Electronic) IS - 0258-851X (Linking) VI - 40 IP - 6 DP - 2026 Nov-Dec TI - Galectin-3 as a Candidate Marker of Endocrine-modulator-associated Endometrial Remodeling During Breast Cancer Endocrine Therapy. PG - 3519-3539 LID - 10.21873/invivo.14494 [doi] AB - BACKGROUND/AIM: Breast cancer endocrine therapies may induce endometrial alterations, but the endometrial effects of antiprogestins remain incompletely defined. This study evaluated the effects of tamoxifen, mifepristone, and ulipristal acetate on endometrial morphology and galectin-3 expression, with the aim of exploring galectin-3 as a candidate marker of endocrine-modulator-associated endometrial remodeling. MATERIALS AND METHODS: Endometrial effects were examined in a rat mammary carcinogenesis model treated with tamoxifen, mifepristone, ulipristal acetate, or vehicle control. Uterine tissues were assessed by histology and galectin-3 immunohistochemistry. Complementary in vitro experiments in Ishikawa endometrial adenocarcinoma cells evaluated agent- and dose-associated galectin-3 protein-expression patterns by western blotting. Histological outcomes and in vitro fold-change values were summarized descriptively. RESULTS: In the analyzed cohort, tamoxifen-treated animals showed the highest descriptive proportion of complex endometrial hyperplasia, whereas mifepristone and ulipristal acetate were associated predominantly with simple hyperplastic changes. Normal endometrial morphology was retained in selected mifepristone- and ulipristal acetate-treated animals and in all control animals. Galectin-3 immunoreactivity was low but detectable in morphologically normal endometrium, whereas hyperplastic lesions showed no detectable galectin-3 immunoreactivity under the staining conditions used. In Ishikawa cells, galectin-3 expression showed agent-specific fold-change patterns: tamoxifen and mifepristone were associated with reduced expression at higher concentrations, while ulipristal acetate showed a non-monotonic pattern with increased expression at low and intermediate concentrations and return toward baseline at the highest concentration. CONCLUSION: In this exploratory model, endocrine-modulator-associated hyperplastic endometrial remodeling was accompanied by reduced or undetectable galectin-3 immunoreactivity, while low-level expression was retained in morphologically normal endometrium. These findings support further investigation of galectin-3 as a candidate marker of endocrine-associated endometrial remodeling, but validation in larger animal cohorts and human endometrial samples from treated patients is required. CI - Copyright (c) 2026, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Kontogeorgi, Adamantia AU - Kontogeorgi A AD - Medical School, University of Crete, Heraklion, Greece; adamantia.kondogewrgi@gmail.com. FAU - Boutas, Ioannis AU - Boutas I AD - Breast Unit, Rea Maternity Hospital, Athens, Greece. FAU - Kalaitzis, Dimitrios AU - Kalaitzis D AD - Independent Authority of Public Revenue, Athens, Greece. FAU - Driva, Tatiana S AU - Driva TS AD - 1 Department of Pathology, General Hospital of Athens "Laiko", Medical School, National and Kapodistrian University of Athens, Athens, Greece. FAU - Agrogiannis, George AU - Agrogiannis G AD - 1 Department of Propedeutic Surgery, General Hospital of Athens "Laiko", Medical School, National and Kapodistrian University of Athens, Athens, Greece. FAU - Zoumakis, Emmanouil AU - Zoumakis E AD - 1 Department of Pediatrics, Medical School, General Hospital of Athens "Agia Sofia", Medical School, National and Kapodistrian University of Athens, Athens, Greece. FAU - Kalantaridou, Sophia N AU - Kalantaridou SN AD - Medical School, National and Kapodistrian University of Athens, Athens, Greece. FAU - Makrigiannakis, Antonios AU - Makrigiannakis A AD - Department of Obstetrics and Gynecology, University of Crete, Heraklion, Greece. LA - eng PT - Journal Article PL - Greece TA - In Vivo JT - In vivo (Athens, Greece) JID - 8806809 RN - 0 (Galectin 3) RN - 094ZI81Y45 (Tamoxifen) RN - 0 (Antineoplastic Agents, Hormonal) RN - 320T6RNW1F (Mifepristone) RN - 0 (Biomarkers) RN - 0 (Biomarkers, Tumor) SB - IM MH - Female MH - Animals MH - *Galectin 3/metabolism/genetics MH - Rats MH - *Endometrium/drug effects/metabolism/pathology MH - Humans MH - Tamoxifen/adverse effects/administration & dosage MH - *Antineoplastic Agents, Hormonal/adverse effects/pharmacology MH - *Breast Neoplasms/drug therapy/pathology/metabolism MH - Mifepristone/administration & dosage MH - Biomarkers MH - Cell Line, Tumor MH - Biomarkers, Tumor MH - Immunohistochemistry OTO - NOTNLM OT - Galectin-3 OT - antiprogestins OT - breast cancer OT - endocrine therapy OT - endometrial hyperplasia EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 20:53 PHST- 2026/07/02 00:00 [received] PHST- 2026/07/19 00:00 [revised] PHST- 2026/07/29 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - 40/6/3519 [pii] AID - 10.21873/invivo.14494 [doi] PST - ppublish SO - In Vivo. 2026 Nov-Dec;40(6):3519-3539. doi: 10.21873/invivo.14494. PMID- 42957236 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1791-7549 (Electronic) IS - 0258-851X (Linking) VI - 40 IP - 6 DP - 2026 Nov-Dec TI - Salvage Chemotherapy After Pembrolizumab for Recurrent or Metastatic Head and Neck Squamous Cell Carcinoma. PG - 3648-3656 LID - 10.21873/invivo.14503 [doi] AB - BACKGROUND/AIM: Pembrolizumab-based therapy is widely used for recurrent or metastatic head and neck squamous cell carcinoma (R/M HNSCC). However, clinical outcomes after subsequent salvage chemotherapy (SCT) remain insufficiently defined. This study evaluated the efficacy of SCT after pembrolizumab in patients with R/M HNSCC. PATIENTS AND METHODS: This multicenter retrospective study included 36 evaluable patients with R/M HNSCC who received SCT after pembrolizumab between December 2019 and February 2022. The primary endpoint was the objective response rate. Secondary endpoints included the disease control rate, progression-free survival, overall survival, and exploratory clinical factors associated with treatment outcomes. RESULTS: The best overall response to SCT was a complete response in 2 patients, partial response in 8, stable disease in 12, and progressive disease in 14. The overall response rate was 27.8%, and the disease control rate was 61.1%. Median progression-free and overall survival were 4.6 months and 11.5 months, respectively. The overall response rate was significantly higher (>4-fold) in patients with prior surgery and in platinum-naive patients. A combined positive score of 20 or higher for tumor programmed cell death-ligand 1, initial M1 status, and prior surgery were associated with longer progression-free survival in exploratory analyses. CONCLUSION: SCT after pembrolizumab showed clinically meaningful activity in selected patients with R/M HNSCC. These findings support SCT as a reasonable post-pembrolizumab option, although prospective validation is warranted. CI - Copyright (c) 2026, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Fushimi, Chihiro AU - Fushimi C AD - Department of Head and Neck Oncology and Surgery, International University of Health and Welfare Mita Hospital, Tokyo, Japan; chfushim@ncc.go.jp. AD - Department of Head and Neck Surgery, National Cancer Center Hospital, Tokyo, Japan. FAU - Okamoto, Isaku AU - Okamoto I AD - Department of Otorhinolaryngology, Head and Neck Surgery, Tokyo Medical University, Tokyo, Japan. FAU - Matsuki, Takashi AU - Matsuki T AD - Department of Otorhinolaryngology, Head and Neck Surgery, Kitasato University School of Medicine, Kanagawa, Japan. AD - Department of Head and Neck Surgery, Kanagawa Cancer Center, Kanagawa, Japan. FAU - Okada, Takuro AU - Okada T AD - Department of Otorhinolaryngology, Head and Neck Surgery, Tokyo Medical University, Tokyo, Japan. FAU - Ueda, Yuri AU - Ueda Y AD - Department of Otorhinolaryngology, Head and Neck Surgery, Tokyo Medical University, Tokyo, Japan. FAU - Takahashi, Hideaki AU - Takahashi H AD - Department of Head and Neck Surgery, National Cancer Center Hospital, Tokyo, Japan. AD - Department of Otorhinolaryngology, Head and Neck Surgery, Yokohama City University, Kanagawa, Japan. FAU - Sato, Hiroki AU - Sato H AD - Department of Otorhinolaryngology, Head and Neck Surgery, Tokyo Medical University, Tokyo, Japan. FAU - Kondo, Takahito AU - Kondo T AD - Department of Otorhinolaryngology, Head and Neck Surgery, Tokyo Medical University, Tokyo, Japan. FAU - Tokashiki, Kunihiko AU - Tokashiki K AD - Department of Otorhinolaryngology, Head and Neck Surgery, Tokyo Medical University, Tokyo, Japan. FAU - Hanyu, Kenji AU - Hanyu K AD - Department of Otorhinolaryngology, Head and Neck Surgery, Tokyo Medical University, Tokyo, Japan. FAU - Ito, Tatsuya AU - Ito T AD - Department of Otorhinolaryngology, Head and Neck Surgery, Tokyo Medical University, Tokyo, Japan. FAU - Kushihashi, Yukiomi AU - Kushihashi Y AD - Department of Head and Neck Surgery, Chibanishi General Hospital, Chiba, Japan. FAU - Masubuchi, Tatsuo AU - Masubuchi T AD - Department of Head and Neck Surgery, Chibanishi General Hospital, Chiba, Japan. FAU - Tada, Yuichiro AU - Tada Y AD - Department of Head and Neck Oncology and Surgery, International University of Health and Welfare Mita Hospital, Tokyo, Japan. AD - Department of Otorhinolaryngology-Head and Neck Surgery, Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital, Tokyo, Japan. FAU - Omura, G O AU - Omura GO AD - Department of Head and Neck Surgery, National Cancer Center Hospital, Tokyo, Japan. FAU - Yamashita, Taku AU - Yamashita T AD - Department of Otorhinolaryngology, Head and Neck Surgery, Kitasato University School of Medicine, Kanagawa, Japan. FAU - Oridate, Nobuhiko AU - Oridate N AD - Department of Otorhinolaryngology, Head and Neck Surgery, Yokohama City University, Kanagawa, Japan. FAU - Tsukahara, Kiyoaki AU - Tsukahara K AD - Department of Otorhinolaryngology, Head and Neck Surgery, Tokyo Medical University, Tokyo, Japan. LA - eng PT - Journal Article PT - Multicenter Study PL - Greece TA - In Vivo JT - In vivo (Athens, Greece) JID - 8806809 RN - 0 (Antibodies, Monoclonal, Humanized) RN - DPT0O3T46P (pembrolizumab) RN - 0 (Antineoplastic Agents, Immunological) SB - IM MH - Humans MH - *Antibodies, Monoclonal, Humanized/administration & dosage/therapeutic use/adverse effects MH - *Salvage Therapy/methods MH - Female MH - Male MH - *Squamous Cell Carcinoma of Head and Neck/drug therapy/pathology/mortality MH - Middle Aged MH - *Neoplasm Recurrence, Local/drug therapy/pathology MH - Aged MH - Neoplasm Metastasis MH - *Antineoplastic Agents, Immunological/therapeutic use/adverse effects/administration & dosage MH - Retrospective Studies MH - Treatment Outcome MH - Adult MH - *Head and Neck Neoplasms/drug therapy/pathology MH - *Antineoplastic Combined Chemotherapy Protocols/therapeutic use/adverse effects OTO - NOTNLM OT - Pembrolizumab OT - cetuximab OT - head and neck cancer OT - paclitaxel OT - recurrent/metastatic OT - salvage chemotherapy EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 20:53 PHST- 2026/07/13 00:00 [received] PHST- 2026/08/02 00:00 [revised] PHST- 2026/08/05 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - 40/6/3648 [pii] AID - 10.21873/invivo.14503 [doi] PST - ppublish SO - In Vivo. 2026 Nov-Dec;40(6):3648-3656. doi: 10.21873/invivo.14503. PMID- 42957234 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1791-7549 (Electronic) IS - 0258-851X (Linking) VI - 40 IP - 6 DP - 2026 Nov-Dec TI - Surface Roughness as a Determinant of the Immunological Tissue Response to Additively Manufactured Titanium Implants. PG - 3367-3382 LID - 10.21873/invivo.14483 [doi] AB - BACKGROUND/AIM: Surface roughness strongly affects host responses to titanium implants. Additive manufacturing (AM) by selective laser melting (SLM) allows controlled surface generation but the immune consequences of AM-derived roughness remain unclear. MATERIALS AND METHODS: Three AM titanium disks with distinct roughness were analyzed by scanning electron microscopy and profilometry. At 10 and 30 days after subcutaneous implantation of disks in rats, tissue responses were evaluated according to ISO 10993-6. A novel phagocyte accumulation index (PAI) was applied, integrating mononuclear infiltration, multinucleated giant cells, coverage, and morphology. RESULTS: All specimens induced only mild to moderate inflammation, confirming biocompatibility. Histology showed fibrosis, macrophage persistence, and rare multinucleated giant cells, without intergroup differences by ISO scoring. In contrast, the PAI detected significant differences: high-roughness specimens showed the highest values at day 30, significantly exceeding those of medium-roughness surfaces (p<0.05), while low-roughness surfaces were intermediate in value. CONCLUSION: The PAI revealed surface roughness-dependent differences in phagocytic activity not captured by ISO scoring. High-roughness AM surfaces promoted the strongest response, highlighting AM and its process parameters as a biological determinant and PAI as a sensitive tool for biomaterial evaluation. CI - Copyright (c) 2026, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Barbeck, Mike AU - Barbeck M AD - Clinic and Policlinic for Dermatology and Venereology, University Medical Center Rostock, Rostock, Germany; mike.barbeck@med.uni-rostock.de. FAU - Pantermehl, Sven AU - Pantermehl S AD - Clinic and Policlinic for Dermatology and Venereology, University Medical Center Rostock, Rostock, Germany. FAU - Stammkoetter, Julia AU - Stammkoetter J AD - Clinic and Policlinic for Dermatology and Venereology, University Medical Center Rostock, Rostock, Germany. FAU - Reinholdt, Cindy AU - Reinholdt C AD - Clinic and Policlinic for Dermatology and Venereology, University Medical Center Rostock, Rostock, Germany. FAU - Stammkoetter, Sebastian AU - Stammkoetter S AD - Chair of Materials Test Engineering (WPT), TU Dortmund University, Dortmund, Germany. FAU - Jahn, Anne AU - Jahn A AD - Additive Manufacturing Department, Laser Zentrum Hannover e.V., Hannover, Germany. FAU - Lingnau, Lars AU - Lingnau L AD - Chair of Materials Test Engineering (WPT), TU Dortmund University, Dortmund, Germany. FAU - Stojanovic, Sanja AU - Stojanovic S AD - Scientific Research Center for Biomedicine, Department for Cell and Tissue Engineering, Faculty of Medicine, University of Nis, Nis, Serbia. AD - Department of Biology and Human Genetics, Faculty of Medicine, University of Nis, Nis, Serbia. FAU - Najman, Stevo AU - Najman S AD - Scientific Research Center for Biomedicine, Department for Cell and Tissue Engineering, Faculty of Medicine, University of Nis, Nis, Serbia. AD - Department of Biology and Human Genetics, Faculty of Medicine, University of Nis, Nis, Serbia. FAU - Kaierle, Stefan AU - Kaierle S AD - Additive Manufacturing Department, Laser Zentrum Hannover e.V., Hannover, Germany. FAU - Walther, Frank AU - Walther F AD - Chair of Materials Test Engineering (WPT), TU Dortmund University, Dortmund, Germany. FAU - Jung, Ole AU - Jung O AD - Clinic and Policlinic for Dermatology and Venereology, University Medical Center Rostock, Rostock, Germany. LA - eng PT - Journal Article PL - Greece TA - In Vivo JT - In vivo (Athens, Greece) JID - 8806809 RN - D1JT611TNE (Titanium) RN - 0 (Biocompatible Materials) SB - IM MH - *Titanium/chemistry/adverse effects MH - Animals MH - Surface Properties MH - Rats MH - *Prostheses and Implants/adverse effects MH - *Biocompatible Materials/chemistry MH - Macrophages/immunology MH - Phagocytosis MH - Materials Testing MH - Foreign-Body Reaction/immunology MH - Male MH - Microscopy, Electron, Scanning OTO - NOTNLM OT - Additive manufacturing OT - biocompatibility OT - biomaterial immunology OT - foreign-body reaction OT - macrophages OT - surface roughness EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 20:53 PHST- 2026/02/28 00:00 [received] PHST- 2026/07/27 00:00 [revised] PHST- 2026/07/31 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - 40/6/3367 [pii] AID - 10.21873/invivo.14483 [doi] PST - ppublish SO - In Vivo. 2026 Nov-Dec;40(6):3367-3382. doi: 10.21873/invivo.14483. PMID- 42957224 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1791-7549 (Electronic) IS - 0258-851X (Linking) VI - 40 IP - 6 DP - 2026 Nov-Dec TI - Renal and Vascular Effects of Selective FGFR4 Inhibition in Folic Acid-induced Kidney Injury in Rats. PG - 3591-3606 LID - 10.21873/invivo.14498 [doi] AB - BACKGROUND/AIM: Fibroblast growth factor-23 (FGF-23) has been implicated in chronic kidney disease (CKD)-associated cardiovascular dysfunction, potentially through fibroblast growth factor receptor 4 (FGFR4). This study investigated renal and vascular alterations in folic acid-induced CKD and evaluated the effects of selective FGFR4 inhibition. MATERIALS AND METHODS: Male Sprague-Dawley rats were assigned to healthy control, CKD control, vehicle, and roblitinib-treated groups. CKD was induced by a single intraperitoneal dose of folic acid. Serum creatinine, FGF-23, tumor necrosis factor-alpha, interleukin-1beta, and asymmetric dimethylarginine levels were measured. Vascular reactivity was evaluated in isolated aortic rings using phenylephrine, carbachol, and sodium nitroprusside. Renal fibrosis, carotid calcification, and medial thickness were assessed histologically. RESULTS: Folic acid administration increased serum creatinine and relative kidney weight and impaired both endothelium-dependent and endothelium-independent vascular relaxation. Relative kidney weight was lower in the roblitinib-treated group than in the CKD control group. However, changes in vascular responses observed in both the treatment and vehicle groups prevented these effects from being attributed specifically to FGFR4 inhibition. FGF-23 and inflammatory marker levels did not differ significantly among the groups. Carotid medial thickness was significantly greater in the treatment group than in the CKD-control group (p<0.05), whereas no other significant between-group differences were detected. CONCLUSION: Folic acid-induced renal injury was associated with early functional vascular impairment despite limited structural and inflammatory alterations. Selective FGFR4 inhibition did not demonstrate a clear therapeutic effect. Further in vivo studies using complementary CKD models, direct assessment of FGFR4 signaling, and vehicle formulations without intrinsic vascular activity are required to validate these findings. CI - Copyright (c) 2026, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Rumanli, Zeliha AU - Rumanli Z AD - Department of Pharmacology, University of Health Sciences, Gulhane Faculty of Pharmacy, Ankara, Turkiye; zeliha.rumanli@sbu.edu.tr. FAU - Avci, Emre AU - Avci E AD - Department of Biochemistry, University of Health Sciences, Gulhane Faculty of Pharmacy, Ankara, Turkiye. FAU - Yilmaz, Ulku Irem AU - Yilmaz UI AD - Department of Pathology, University of Health Sciences, Gulhane Vocational School of Health, Ankara, Turkiye. FAU - Baykal, Baris AU - Baykal B AD - Department of Histology and Embryology, University of Health Sciences, Gulhane Faculty of Medicine, Ankara, Turkiye. FAU - Yildirim, Beyhan Karli AU - Yildirim BK AD - Department of Histology and Embryology, University of Health Sciences, Gulhane Faculty of Medicine, Ankara, Turkiye. FAU - Aksoy, Okan Ali AU - Aksoy OA AD - Gulhane Institute of Health Sciences, University of Health Sciences, Ankara, Turkiye. FAU - Goksel, Berk Alp AU - Goksel BA AD - Gulhane Institute of Health Sciences, University of Health Sciences, Ankara, Turkiye. FAU - Vural, Ismail Mert AU - Vural IM AD - Department of Pharmacology, University of Health Sciences, Gulhane Faculty of Pharmacy, Ankara, Turkiye. LA - eng PT - Journal Article PL - Greece TA - In Vivo JT - In vivo (Athens, Greece) JID - 8806809 RN - EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 4) RN - 935E97BOY8 (Folic Acid) RN - 62031-54-3 (Fibroblast Growth Factors) RN - 7Q7P4S7RRE (Fibroblast Growth Factor-23) RN - 0 (Biomarkers) SB - IM MH - Animals MH - Rats MH - *Receptor, Fibroblast Growth Factor, Type 4/antagonists & inhibitors/metabolism MH - Male MH - *Folic Acid/adverse effects MH - *Kidney/drug effects/pathology/metabolism MH - Fibroblast Growth Factors/metabolism/blood MH - Disease Models, Animal MH - *Renal Insufficiency, Chronic/metabolism/chemically induced/pathology/drug therapy MH - Rats, Sprague-Dawley MH - Fibroblast Growth Factor-23 MH - Biomarkers OTO - NOTNLM OT - Chronic kidney disease OT - FGF-23 OT - FGFR4 OT - endothelial dysfunction OT - roblitinib EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 20:53 PHST- 2026/08/15 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - 40/6/3591 [pii] AID - 10.21873/invivo.14498 [doi] PST - ppublish SO - In Vivo. 2026 Nov-Dec;40(6):3591-3606. doi: 10.21873/invivo.14498. PMID- 42957220 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1791-7549 (Electronic) IS - 0258-851X (Linking) VI - 40 IP - 6 DP - 2026 Nov-Dec TI - Suspected Donor-derived Myeloid Neoplasia After Allogeneic HSCT: A Case-based Review of Clonal Evolution. PG - 3900-3911 LID - 10.21873/invivo.14527 [doi] AB - BACKGROUND/AIM: Donor-derived leukemia represents a rare but increasingly recognized clinical complication of allogeneic hematopoietic stem cell transplantation. Emerging evidence indicates that clonal hematopoiesis of indeterminate potential in donors provides the biological substrate for donor-derived leukemia, while post-transplant immune pressure and niche damage drive clonal selection and leukemic evolution. CASE REPORT: We describe the case of a 70-year-old woman with acute myeloid leukemia who underwent matched unrelated donor allogeneic hematopoietic stem cell transplantation (allo-HSCT) from a 25-year-old female donor. Following transplantation, serial bone marrow examinations demonstrated persistent predominant donor chimerism accompanied by progressive expansion of an aberrant myeloid population, increasing from approximately 2% to 14% by multiparametric flow cytometry, without clear morphologic relapse. Targeted next-generation sequencing at hematologic progression identified a pathogenic RAS-pathway mutation with a variant allele frequency of 7%, while no molecular features overlapping with the initially characterized acute myelogenous leukemia (AML) were identified. However, pre-transplant next-generation sequencing (NGS) data from both donor and recipient and lineage-specific chimerism of the abnormal population were unavailable, preventing definitive demonstration of donor origin. CONCLUSION: This case represents a suspected donor-derived myeloid neoplasm arising after allo-HSCT and illustrates the diagnostic difficulty of distinguishing donor-derived disease from relapse and other post-transplant myeloid neoplasms. The case also highlights the potential value of integrating serial flow cytometry, molecular profiling, and lineage-specific chimerism analyses when unexplained cytopenias or progressive abnormal myeloid populations develop despite preserved donor chimerism. The proposed evolutionary and microenvironmental mechanisms should be considered hypothesis-generating and require further validation in molecularly characterized cases. CI - Copyright (c) 2026, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Mirabile, Giuseppe AU - Mirabile G AD - Hematology Unit, Department of Human Pathology in Adulthood and Childhood "Gaetano Barresi", University Hospital "G. Martino" Messina, Messina, Italy. FAU - Bono, Roberto AU - Bono R AD - Bone Marrow Transplant Unit, Azienda Ospedaliera Ospedali Riuniti Villa Sofia Cervello, Palermo, Italy. FAU - Bottaro, Adele AU - Bottaro A AD - Hematology Unit, Department of Human Pathology in Adulthood and Childhood "Gaetano Barresi", University Hospital "G. Martino" Messina, Messina, Italy. FAU - Stagno, Fabio AU - Stagno F AD - Hematology Unit, Department of Human Pathology in Adulthood and Childhood "Gaetano Barresi", University Hospital "G. Martino" Messina, Messina, Italy. FAU - Coppolino, Giuseppe AU - Coppolino G AD - Hematology Unit, Department of Human Pathology in Adulthood and Childhood "Gaetano Barresi", University Hospital "G. Martino" Messina, Messina, Italy. FAU - Fazio, Manlio AU - Fazio M AD - Hematology Unit, Department of Human Pathology in Adulthood and Childhood "Gaetano Barresi", University Hospital "G. Martino" Messina, Messina, Italy. FAU - Castagna, Luca AU - Castagna L AD - Bone Marrow Transplant Unit, Azienda Ospedaliera Ospedali Riuniti Villa Sofia Cervello, Palermo, Italy. FAU - Allegra, Alessandro AU - Allegra A AD - Hematology Unit, Department of Human Pathology in Adulthood and Childhood "Gaetano Barresi", University Hospital "G. Martino" Messina, Messina, Italy; aallegra@unime.it. LA - eng PT - Case Reports PT - Journal Article PT - Review PL - Greece TA - In Vivo JT - In vivo (Athens, Greece) JID - 8806809 SB - IM MH - Humans MH - *Hematopoietic Stem Cell Transplantation/adverse effects/methods MH - Female MH - *Clonal Evolution/genetics MH - Transplantation, Homologous/adverse effects MH - Aged MH - *Leukemia, Myeloid, Acute/etiology/therapy/diagnosis/pathology MH - *Tissue Donors MH - High-Throughput Nucleotide Sequencing MH - Mutation OTO - NOTNLM OT - Donor-derived leukemia OT - allogeneic hematopoietic stem cell transplantation OT - bone marrow chimerism OT - bone marrow niche OT - clonal evolution OT - clonal hematopoiesis of indeterminate potential (CHIP) OT - immune selection EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 20:53 PHST- 2026/07/25 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - 40/6/3900 [pii] AID - 10.21873/invivo.14527 [doi] PST - ppublish SO - In Vivo. 2026 Nov-Dec;40(6):3900-3911. doi: 10.21873/invivo.14527. PMID- 42957210 OWN - NLM STAT- Publisher LR - 20261029 IS - 1535-5667 (Electronic) IS - 0161-5505 (Linking) DP - 2026 Oct 29 TI - Impact of PSMA PET/CT Staging on Long-Term Outcomes in Patients with Clinically Localized Prostate Cancer: Results of a Large Single-Center Cohort. LID - jnumed.126.273261 [pii] LID - 10.2967/jnumed.126.273261 [doi] AB - Prostate-specific membrane antigen (PSMA) PET/CT has become the gold standard for staging and restaging prostate cancer, with superior diagnostic accuracy over conventional imaging and established prognostic value. However, there are limited data on whether PSMA PET/CT-improved staging accuracy is associated with improved long-term patient outcomes. This study analyzes the association between PSMA PET/CT at primary staging and overall survival (OS), biochemical recurrence (BCR), and cancer-specific survival (CSS) in a large university hospital cohort at a center where PSMA PET/CT has been clinically available for over 13 y. Methods: This retrospective single-center study included all 11,146 patients with prostate cancer diagnosed between 2013 and 2023. The final analysis cohort consisted of 6999 patients, of whom 505 (7.2%) underwent PSMA PET/CT for primary staging. We classified patients into 3 groups using D'Amico risk classification. Survival analyses were performed using Kaplan-Meier methods and adjusted Cox regression models. To control for selection bias, propensity score matching and overlap weighting were applied. Landmark analysis addressed selection and immortal time biases, and Fine-Gray models accounted for competing risk of death in a BCR analysis. Results: PSMA PET/CT was associated with a longer time to BCR (hazard ratio [HR] = 0.51; 95% CI, 0.38-0.68; P < 0.001), longer CSS (HR = 0.37; 95% CI, 0.22-0.61; P < 0.001), and longer OS (HR = 0.54; 95% CI, 0.40-0.70; P < 0.001). PSMA PET imaging was associated with particularly better outcomes in high-risk patients, especially those treated with radiotherapy. In the radiotherapy group, the HRs were 0.42 for OS, 0.42 for BCR, and 0.25 for CSS (all P < 0.001), whereas no statistically significant association was observed in patients undergoing radical prostatectomy. As the formal interaction test between PSMA PET/CT and treatment modality was not statistically significant, this differential finding should be considered exploratory. Across all 9 sensitivity analyses, HRs were consistently below 1, indicating a uniform direction of benefit. Conclusion: The association observed in this large real-world cohort supports the clinical value of PSMA PET/CT for primary staging. Findings were particularly favorable among high-risk patients undergoing radiotherapy, although this subgroup difference should be interpreted as exploratory given the nonsignificant interaction test. These results support a potential role for PSMA PET/CT in improving patient selection and detecting oligometastatic disease, enabling higher precision in treatment planning. CI - (c) 2026 by the Society of Nuclear Medicine and Molecular Imaging. FAU - Esmaeilzadeh Asl, Sima AU - Esmaeilzadeh Asl S AD - Department of Translational Imaging in Oncology, National Center for Tumor Diseases, NCT/UCC Dresden, a partnership between DKFZ, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology and Helmholtz-Zentrum Dresden-Rossendorf, Dresden, Germany. AD - Department of Nuclear Medicine, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany. FAU - Hoberuck, Sebastian AU - Hoberuck S AD - Department of Nuclear Medicine, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany. FAU - Sonnenfeld, Andre AU - Sonnenfeld A AD - Department of Urology, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany. FAU - Stange, Thoralf AU - Stange T AD - National Center for Tumor Diseases Dresden, A Partnership Between DKFZ, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology and HZDR, Dresden, Germany; and. FAU - Bohme, Heiko AU - Bohme H AD - National Center for Tumor Diseases Dresden, A Partnership Between DKFZ, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology and HZDR, Dresden, Germany; and. FAU - Klimova, Anna AU - Klimova A AD - National Center for Tumor Diseases Dresden, A Partnership Between DKFZ, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology and HZDR, Dresden, Germany; and. FAU - Rentsch, Anke AU - Rentsch A AD - National Center for Tumor Diseases Dresden, A Partnership Between DKFZ, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology and HZDR, Dresden, Germany; and. FAU - Holscher, Tobias AU - Holscher T AD - Department of Radiotherapy and Radiation Oncology, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany. FAU - Boehm, Katharina AU - Boehm K AD - Department of Urology, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany. FAU - Thomas, Christian AU - Thomas C AD - Department of Urology, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany. FAU - Miederer, Matthias AU - Miederer M AD - Department of Translational Imaging in Oncology, National Center for Tumor Diseases, NCT/UCC Dresden, a partnership between DKFZ, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology and Helmholtz-Zentrum Dresden-Rossendorf, Dresden, Germany; matthias.miederer@nct-dresden.de. AD - Department of Nuclear Medicine, Faculty of Medicine and University Hospital Carl Gustav Carus, TUD Dresden University of Technology, Dresden, Germany. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Nucl Med JT - Journal of nuclear medicine : official publication, Society of Nuclear Medicine JID - 0217410 SB - IM OTO - NOTNLM OT - PSMA PET/CT OT - prostate cancer OT - radiotherapy OT - survival analysis EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:53 PHST- 2026/07/02 00:00 [received] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - jnumed.126.273261 [pii] AID - 10.2967/jnumed.126.273261 [doi] PST - aheadofprint SO - J Nucl Med. 2026 Oct 29:jnumed.126.273261. doi: 10.2967/jnumed.126.273261. PMID- 42957209 OWN - NLM STAT- Publisher LR - 20261029 IS - 1535-5667 (Electronic) IS - 0161-5505 (Linking) DP - 2026 Oct 29 TI - Role of [(18)F]F-Fluciclovine PET/CT in Patients with Biochemical Recurrence of Prostate Cancer and Negative PSMA PET Results: A Single-Center Study. LID - jnumed.126.272834 [pii] LID - 10.2967/jnumed.126.272834 [doi] AB - Our purpose was to evaluate whether [(18)F]F-fluciclovine PET/CT detects sites of disease in patients with biochemical recurrence (BCR) of prostate cancer and negative findings on prostate-specific membrane antigen (PSMA) PET and to assess its impact on intended management. Methods: In this prospective single-arm study (NCT05722925), 50 patients with BCR (prostate-specific antigen [PSA] >/= 0.2 ng/mL) and negative findings on PSMA PET underwent [(18)F]F-fluciclovine PET/CT within 30 d of the previous imaging. Detection rate, distribution of disease, PSA-stratified analysis, management impact, and correct localization rate were assessed using descriptive statistics and exact CI. Results: [(18)F]F-fluciclovine PET had positive findings in 28 of 50 patients (56%; 95% CI, 41%-70%). Disease was confined to the prostate bed in 14 patients (50%), whereas 14 (50%) demonstrated nodal or distant metastases. Detection rate increased with PSA, from 44% at a PSA of 0.2-0.5 ng/mL to 100% at a PSA of 5 ng/mL or higher. The intended management changed in 71% of patients after the [(18)F]F-fluciclovine PET: 87% of patients with positive findings and 54% with negative findings. Among patients with an available reference standard (n = 14), all positive findings were confirmed to be true-positive. Conclusion: [(18)F]F-fluciclovine PET detected recurrent disease in over half of patients with negative PSMA PET findings and frequently altered management, supporting its complementary role in selected patients with BCR. CI - (c) 2026 by the Society of Nuclear Medicine and Molecular Imaging. FAU - Szidonya, Laszlo AU - Szidonya L AD - Department of Diagnostic Radiology, Oregon Health & Science University, Portland, Oregon. FAU - Obrzut, Sebastian AU - Obrzut S AD - Department of Diagnostic Radiology, Oregon Health & Science University, Portland, Oregon. FAU - Lim, Jeong Y AU - Lim JY AD - Biostatistics Shared Resource, Knight Cancer Institute, Oregon Health & Science University, Portland, Oregon; and. FAU - Latour, Emile AU - Latour E AD - Biostatistics Shared Resource, Knight Cancer Institute, Oregon Health & Science University, Portland, Oregon; and. FAU - Choudhary, Gagandeep AU - Choudhary G AD - Department of Diagnostic Radiology, Oregon Health & Science University, Portland, Oregon. FAU - Hung, Arthur AU - Hung A AD - Department of Radiation Oncology, Oregon Health & Science University, Portland, Oregon. FAU - Mallak, Nadine AU - Mallak N AD - Department of Diagnostic Radiology, Oregon Health & Science University, Portland, Oregon; mallak@ohsu.edu. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Nucl Med JT - Journal of nuclear medicine : official publication, Society of Nuclear Medicine JID - 0217410 SB - IM OTO - NOTNLM OT - PSMA OT - biochemical recurrence OT - fluciclovine OT - molecular imaging OT - prostate cancer EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:53 PHST- 2026/04/22 00:00 [received] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - jnumed.126.272834 [pii] AID - 10.2967/jnumed.126.272834 [doi] PST - aheadofprint SO - J Nucl Med. 2026 Oct 29:jnumed.126.272834. doi: 10.2967/jnumed.126.272834. PMID- 42957206 OWN - NLM STAT- Publisher LR - 20261029 IS - 1535-5667 (Electronic) IS - 0161-5505 (Linking) DP - 2026 Oct 29 TI - Beyond Prostate-Specific Antigen Response: Could (18)F-FDG PET Refine Patient Selection for Rechallenge with [(177)Lu]Lu-PSMA-617? LID - jnumed.126.273292 [pii] LID - 10.2967/jnumed.126.273292 [doi] FAU - Cinar, Alev AU - Cinar A LA - eng PT - Letter DEP - 20261029 PL - United States TA - J Nucl Med JT - Journal of nuclear medicine : official publication, Society of Nuclear Medicine JID - 0217410 SB - IM EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:53 PHST- 2026/06/25 00:00 [received] PHST- 2026/07/15 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - jnumed.126.273292 [pii] AID - 10.2967/jnumed.126.273292 [doi] PST - aheadofprint SO - J Nucl Med. 2026 Oct 29:jnumed.126.273292. doi: 10.2967/jnumed.126.273292. PMID- 42957204 OWN - NLM STAT- Publisher LR - 20261029 IS - 1535-5667 (Electronic) IS - 0161-5505 (Linking) DP - 2026 Oct 29 TI - Predicting [(90)Y]Y-FAPI-46 Tumor Absorbed Dose from Single-Time-Point [(68)Ga]Ga-FAPI-46 PET/CT Imaging. LID - jnumed.126.273258 [pii] LID - 10.2967/jnumed.126.273258 [doi] AB - Radiolabeled fibroblast activation protein (FAP)-targeting peptides, such as (90)Y-FAPI-46, have increasingly been used to treat various tumor entities, with preliminary reports indicating therapeutic responses with acceptable toxicity profiles. Dosimetry can be used to maximize antitumor capability and minimize side effects. The aim of this study was to investigate whether (90)Y-FAPI-46 tumor dosimetry can be predicted by diagnostic (68)Ga-FAPI-46 PET/CT data. Methods: Fourteen patients with 24 lesions underwent a single-time-point (68)Ga-FAPI-46 PET/CT scan and 3 (90)Y-FAPI-46 PET/CT scans for dosimetry within a 3-mo interval. Tumors were identified on PET images, and the anatomic tumor volume was measured on low-dose CT. Tumor uptake extrapolated at time of injection, time-integrated activity coefficient (TIAC), and tumor absorbed dose were calculated as reference values using (90)Y-FAPI-46 data and compared with the corresponding predicted values using (68)Ga-FAPI-46 data by means of a single-time-point dosimetry model. Correlations between (90)Y-FAPI-46- and (68)Ga-FAPI-46-based values were assessed. Results: In calculating the predicted values using a single-time-point (68)Ga-FAPI-46 PET/CT, the mean effective half-life of (90)Y-FAPI-46 across all lesions was 8.44 +/- 1.73 h. The mean +/- SD ratio (i.e., the predicted values using (68)Ga-FAPI-46 data divided by the reference (90)Y-FAPI-46 data) were 0.93 +/- 0.39, 0.77 +/- 0.35, and 0.77 +/- 0.35 for tumor uptake, TIAC, and tumor absorbed dose, respectively. In the Bland-Altman analysis, mean differences (bias) +/- SD of the percent differences of tumor uptake, TIAC, and tumor absorbed dose were -16.11% +/- 43.28% (95% CI, -100.90 to 68.73%; Spearman rho = 0.91; P < 0.0001), -32.60% +/- 41.26% (95% CI, -113.50 to 48.26%; Spearman rho = 0.91; P < 0.0001), and -33.35% +/- 40.19% (95% CI, -112.10 to 45.43%; Spearman rho = 0.77; P < 0.0001), respectively. Conclusion: Tumor uptake, TIAC, and tumor absorbed dose on (90)Y-FAPI-46 PET/CT and (68)Ga-FAPI-46 PET/CT were highly correlated, although the values on (68)Ga-FAPI-46 PET/CT tended to be lower than those on (90)Y-FAPI-46 PET/CT. Predicted values on (68)Ga-FAPI-46 PET/CT can help clinicians with diagnostic stratification and patient selection, with intratherapeutic verification remaining necessary before any individualization of administered activity. CI - (c) 2026 by the Society of Nuclear Medicine and Molecular Imaging. FAU - Watanabe, Masao AU - Watanabe M AD - Department of Nuclear Medicine, West German Cancer Center, University Hospital Essen, University of Duisburg-Essen, Essen, Germany; and. AD - German Cancer Consortium, Partner Site University Hospital Essen, Essen, Germany. FAU - Jentzen, Walter AU - Jentzen W AD - Department of Nuclear Medicine, West German Cancer Center, University Hospital Essen, University of Duisburg-Essen, Essen, Germany; and walter.jentzen@uk-essen.de. AD - German Cancer Consortium, Partner Site University Hospital Essen, Essen, Germany. FAU - Herrmann, Ken AU - Herrmann K AD - Department of Nuclear Medicine, West German Cancer Center, University Hospital Essen, University of Duisburg-Essen, Essen, Germany; and. AD - German Cancer Consortium, Partner Site University Hospital Essen, Essen, Germany. FAU - Kersting, David AU - Kersting D AD - Department of Nuclear Medicine, West German Cancer Center, University Hospital Essen, University of Duisburg-Essen, Essen, Germany; and. AD - German Cancer Consortium, Partner Site University Hospital Essen, Essen, Germany. FAU - Fragoso Costa, Pedro AU - Fragoso Costa P AD - Department of Nuclear Medicine, West German Cancer Center, University Hospital Essen, University of Duisburg-Essen, Essen, Germany; and. AD - German Cancer Consortium, Partner Site University Hospital Essen, Essen, Germany. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Nucl Med JT - Journal of nuclear medicine : official publication, Society of Nuclear Medicine JID - 0217410 SB - IM OTO - NOTNLM OT - 68Ga-FAPI-46 PET/CT OT - 90Y-FAPI-46 PET/CT OT - absorbed dose OT - time-integrated activity coefficient OT - tumor uptake EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:53 PHST- 2026/06/18 00:00 [received] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - jnumed.126.273258 [pii] AID - 10.2967/jnumed.126.273258 [doi] PST - aheadofprint SO - J Nucl Med. 2026 Oct 29:jnumed.126.273258. doi: 10.2967/jnumed.126.273258. PMID- 42957200 OWN - NLM STAT- Publisher LR - 20261029 IS - 1535-5667 (Electronic) IS - 0161-5505 (Linking) DP - 2026 Oct 29 TI - Reply to "Beyond Prostate-Specific Antigen Response: Could (18)F-FDG PET Refine Patient Selection for Rechallenge with [(177)Lu]Lu-PSMA-617?". LID - jnumed.126.274063 [pii] LID - 10.2967/jnumed.126.274063 [doi] FAU - Calais, Jeremie AU - Calais J CN - RE-LuPSMA investigators LA - eng PT - Letter DEP - 20261029 PL - United States TA - J Nucl Med JT - Journal of nuclear medicine : official publication, Society of Nuclear Medicine JID - 0217410 SB - IM EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:53 PHST- 2026/10/06 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - jnumed.126.274063 [pii] AID - 10.2967/jnumed.126.274063 [doi] PST - aheadofprint SO - J Nucl Med. 2026 Oct 29:jnumed.126.274063. doi: 10.2967/jnumed.126.274063. PMID- 42957199 OWN - NLM STAT- Publisher LR - 20261029 IS - 1535-5667 (Electronic) IS - 0161-5505 (Linking) DP - 2026 Oct 29 TI - Prostate-Specific Membrane Antigen PET/CT Complements MRI for Focal Therapy Selection in Prostate Cancer: A Whole-Mount Histopathologic Study. LID - jnumed.126.273367 [pii] LID - 10.2967/jnumed.126.273367 [doi] AB - The incremental value of prostate-specific membrane antigen (PSMA) PET/CT over multiparametric MRI (mpMRI) for selecting patients for prostate cancer focal therapy is undefined. Unlike lesion-detection studies, we evaluated whether PSMA PET/CT, mpMRI, and their combination correctly determine focal therapy eligibility when validated against whole-mount histopathology (WMHP). Methods: We retrospectively analyzed patients undergoing radical prostatectomy (RP) at UCLA (2017-2024) with preoperative PSMA PET/CT and mpMRI, a biopsy grade group (GG) of 3 or less, unilateral disease, and a prostate-specific antigen level of less than 20 ng/mL. Two blinded readers per modality assessed extraprostatic extension (EPE), seminal vesicle invasion (SVI), bilateral disease, and multifocality; a third adjudicated discordances. Eligibility required the absence of all 4. On WMHP, ineligibility was EPE, SVI, multifocal or bilateral disease with a contralateral or secondary GG of at least 2 nodules, or any GG greater than 3 focus at RP. Modalities were compared by McNemar tests. Results: Of 112 patients, 77 (69%) were ineligible on WMHP, including 19 (17%) with a GG greater than 3 focus at RP. Sensitivity and specificity were 53% and 54% for PET and 70% and 49% for mpMRI. Combined imaging improved sensitivity to 83% (P < 0.001 vs. PET; P = 0.002 vs. MRI) but reduced specificity to 26%. Per 100 patients at this prevalence, this would correctly identify approximately 9 additional ineligible patients while falsely excluding approximately 7 true candidates. mpMRI was more sensitive than PET for EPE (77% vs. 32%, P < 0.001), whereas PET was more specific (90% vs. 58%, P < 0.001). Combined imaging improved detection of multifocal (44% vs. 23%, P = 0.001) and bilateral disease (45% vs. 23%, P = 0.002) versus MRI alone. Conclusion: Adding PSMA PET/CT to mpMRI significantly improves sensitivity for exclusionary features, reflecting complementary molecular and anatomic information. However, combined imaging still misses a substantial proportion of adverse pathology, so imaging alone should not determine eligibility. CI - (c) 2026 by the Society of Nuclear Medicine and Molecular Imaging. FAU - Sonni, Ida AU - Sonni I AD - Department of Radiological Sciences, David Geffen School of Medicine, UCLA, Los Angeles, California. FAU - Mohammadigoldar, Zahra AU - Mohammadigoldar Z AD - Department of Radiological Sciences, David Geffen School of Medicine, UCLA, Los Angeles, California. FAU - Furey, George AU - Furey G AD - Department of Radiological Sciences, David Geffen School of Medicine, UCLA, Los Angeles, California. FAU - Kimura, Koichiro AU - Kimura K AD - Department of Nuclear Medicine and Theranostics, Ahmanson Translational Theranostics Division, David Geffen School of Medicine, UCLA, Los Angeles, California. FAU - Movva, Pranav C AU - Movva PC AD - Department of Urology, Cedars-Sinai Medical Center, Los Angeles, California. FAU - Holzgreve, Adrien AU - Holzgreve A AD - Department of Nuclear Medicine and Theranostics, Ahmanson Translational Theranostics Division, David Geffen School of Medicine, UCLA, Los Angeles, California. AD - Department of Nuclear Medicine, LMU University Hospital, LMU Munich, Munich, Germany. FAU - Bahri, Shadfar AU - Bahri S AD - Department of Nuclear Medicine and Theranostics, Ahmanson Translational Theranostics Division, David Geffen School of Medicine, UCLA, Los Angeles, California. FAU - Sevco, Tyler AU - Sevco T AD - Department of Radiological Sciences, David Geffen School of Medicine, UCLA, Los Angeles, California. FAU - Chung, Alex AU - Chung A AD - Department of Radiological Sciences, David Geffen School of Medicine, UCLA, Los Angeles, California. FAU - Sisk, Anthony AU - Sisk A AD - Department of Pathology, David Geffen School of Medicine, UCLA, Los Angeles, California. FAU - Grogan, Tristan AU - Grogan T AD - Department of Medicine Statistics Core, UCLA, Los Angeles, California; and. FAU - Czernin, Johannes AU - Czernin J AD - Department of Nuclear Medicine and Theranostics, Ahmanson Translational Theranostics Division, David Geffen School of Medicine, UCLA, Los Angeles, California. FAU - Sung, Kyunghyun AU - Sung K AD - Department of Radiological Sciences, David Geffen School of Medicine, UCLA, Los Angeles, California. FAU - Calais, Jeremie AU - Calais J AD - Department of Nuclear Medicine and Theranostics, Ahmanson Translational Theranostics Division, David Geffen School of Medicine, UCLA, Los Angeles, California. FAU - Eggener, Scott E AU - Eggener SE AD - Department of Urology, David Geffen School of Medicine, UCLA, Los Angeles, California. FAU - Brisbane, Wayne AU - Brisbane W AD - Department of Urology, David Geffen School of Medicine, UCLA, Los Angeles, California. FAU - Reiter, Robert E AU - Reiter RE AD - Department of Urology, David Geffen School of Medicine, UCLA, Los Angeles, California. FAU - Raman, Steven AU - Raman S AD - Department of Radiological Sciences, David Geffen School of Medicine, UCLA, Los Angeles, California. FAU - Weiner, Adam B AU - Weiner AB AD - Department of Urology, Cedars-Sinai Medical Center, Los Angeles, California; adam.weiner@csmc.edu. AD - Department of Urology, David Geffen School of Medicine, UCLA, Los Angeles, California. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Nucl Med JT - Journal of nuclear medicine : official publication, Society of Nuclear Medicine JID - 0217410 SB - IM OTO - NOTNLM OT - PSMA PET/CT OT - focal therapy OT - multiparametric MRI OT - prostate cancer OT - whole-mount histopathology EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:53 PHST- 2026/07/04 00:00 [received] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - jnumed.126.273367 [pii] AID - 10.2967/jnumed.126.273367 [doi] PST - aheadofprint SO - J Nucl Med. 2026 Oct 29:jnumed.126.273367. doi: 10.2967/jnumed.126.273367. PMID- 42957197 OWN - NLM STAT- Publisher LR - 20261029 IS - 1535-5667 (Electronic) IS - 0161-5505 (Linking) DP - 2026 Oct 29 TI - Symptomatic Hyperkinetic Gallbladder: An Unrecognized Indication for Cholecystectomy. LID - jnumed.126.273293 [pii] LID - 10.2967/jnumed.126.273293 [doi] AB - For decades, low gallbladder ejection fraction (GBEF), measured with hepatobiliary scintigraphy after infusion of sincalide, has been used to diagnose symptomatic biliary hypokinesia and as a predictor of the necessity for cholecystectomy. Recently, gallbladder hypercontractility (GBEF > 79%) has been recognized as a cause of right upper quadrant pain, and cholecystectomy has often resulted in symptom relief. This retrospective study evaluated follow-up data collected after GBEF studies to determine the incidence of cholecystectomy or persistence of symptoms. Methods: We evaluated all GBEF biliary studies performed at our institution between January 1, 2012, and December 31, 2025. Study reports containing "ejection fraction" and "sincalide" were identified to establish the study population. All patients presented with right upper quadrant pain. They received 185 MBq (range, 111-259 MBq) of (99m)Tc-mebrofenin intravenously and were imaged in a 20 degrees left anterior oblique position for 60 min. Once the gallbladder was clearly seen, the patients were given a 30-min intravenous infusion of sincalide (0.02 mug/kg), and imaging was continued for a total of 45 min. Patients were categorized as having probable gallbladder disease (cholecystectomy and resolution of symptoms or no cholecystectomy and persistent symptoms) or unlikely gallbladder disease (cholecystectomy and persistent symptoms or no cholecystectomy and resolved symptoms or other diagnosis). Results: In total, 1775 patients were included in the final analysis. Forty percent of patients had a GBEF of greater than 79%, and 27% had a GBEF of less than 39%. Of those who underwent cholecystectomy, 90% of those with a GBEF of less than 39% and 94% of those with a GBEF of greater than 79% experienced symptom relief. The incidence of probable biliary disease was 61% in patients with a low GBEF and 44% in patients with a high GBEF and was lowest in the group with a GBEF of 60%-69% (24% of patients). Conclusion: Our study found that hepatobiliary GBEF is not as accurate as we have assumed. Additional clinical criteria are needed to improve patient selection and positive outcomes. The high percentage of patients with a GBEF exceeding 79% has not been appreciated, as most of the previous studies evaluated healthy subjects and did not examine outcomes. These results should shift how we think about GBEF studies and how the results are reported. CI - (c) 2026 by the Society of Nuclear Medicine and Molecular Imaging. FAU - Graham, Michael M AU - Graham MM AD - Division of Nuclear Medicine, Department of Radiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa; and michael-graham@uiowa.edu. FAU - Mohamed, Mohamed K AU - Mohamed MK AD - Division of Nuclear Medicine, Department of Radiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa; and. FAU - Samuel, Isaac AU - Samuel I AD - Department of Surgery, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Nucl Med JT - Journal of nuclear medicine : official publication, Society of Nuclear Medicine JID - 0217410 SB - IM OTO - NOTNLM OT - cholecystectomy OT - cholecystitis OT - gallbladder OT - gastrointestinal OT - hyperkinetic EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:53 PHST- 2026/06/25 00:00 [received] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - jnumed.126.273293 [pii] AID - 10.2967/jnumed.126.273293 [doi] PST - aheadofprint SO - J Nucl Med. 2026 Oct 29:jnumed.126.273293. doi: 10.2967/jnumed.126.273293. PMID- 42957195 OWN - NLM STAT- Publisher LR - 20261029 IS - 1399-3003 (Electronic) IS - 0903-1936 (Linking) DP - 2026 Oct 29 TI - Don't discard the supernatant: pleural fluid cfDNA for molecular profiling in lung cancer. LID - 2601861 [pii] LID - 10.1183/13993003.01861-2026 [doi] AB - Pleural fluid supernatant cfDNA should be routinely preserved and can already be used selectively for molecular profiling in NSCLC, while broader implementation requires prospective clinical validation. CI - Copyright (c)The authors 2026. For reproduction rights and permissions contact permissions@ersnet.org. FAU - Porcel, Jose M AU - Porcel JM AUID- ORCID: 0000-0002-2734-8061 AD - Pleural Medicine and Ultrasound-Guided Intervention Unit, Department of Internal Medicine, Arnau de Vilanova University Hospital, Lleida, Spain josemporcel@icloud.com. AD - Biomedical Research Institute of Lleida - Dr. Pifarre Foundation, IRBLleida, Lleida, Spain. LA - eng PT - Editorial DEP - 20261029 PL - England TA - Eur Respir J JT - The European respiratory journal JID - 8803460 SB - IM EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:53 PHST- 2026/08/18 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:53 [entrez] AID - 13993003.01861-2026 [pii] AID - 10.1183/13993003.01861-2026 [doi] PST - aheadofprint SO - Eur Respir J. 2026 Oct 29:2601861. doi: 10.1183/13993003.01861-2026. PMID- 42957189 OWN - NLM STAT- Publisher LR - 20261029 IS - 1469-9001 (Electronic) IS - 1355-8382 (Linking) DP - 2026 Oct 29 TI - Cytoplasmic mRNA recapping is essential for stress recovery and intracellular proliferation in Trypanosoma cruzi. LID - rna.081035.126 [pii] LID - 10.1261/rna.081035.126 [doi] AB - Trypanosoma cruzi, the causative agent of Chagas disease, regulates gene expression predominantly after transcription because it lacks conventional gene-specific promoters. Cytoplasmic mRNA recapping has been proposed to restore translation-competent mRNAs from silenced transcripts, but its physiological role in kinetoplastids has remained unclear. Here, we show that the cytoplasmic capping enzyme TcCe1 supports transcriptome homeostasis, an effective heat-shock response, and productive intracellular infection of host cells. TcCe1 loss was associated with reduced transcript abundance across extended polycistronic transcription units, impaired induction of proteostasis factors under stress, altered steady-state abundance of uncapped transcripts detected by ligation-mediated RT-qPCR, and marked defects in recovery and infectivity. Because cytoplasmic recapping in kinetoplastids is mechanistically distinct from host pathways, TcCe1 may represent a promising target for parasite-selective therapeutics. Together, our findings highlight the importance of TcCe1 in RNA-level regulation, environmental responsiveness, and host adaptation in this early-branching eukaryote. CI - Published by Cold Spring Harbor Laboratory Press for the RNA Society. FAU - Nguyen, Kieu Duong My AU - Nguyen KDM AD - Tsukuba Daigaku. FAU - Takagi, Yuko AU - Takagi Y AUID- ORCID: 0000-0002-4567-0986 AD - National Institute of Advanced Industrial Science and Technology. FAU - Kurama, Masae AU - Kurama M AD - National Institute of Advanced Industrial Science and Technology. FAU - Wakamatsu, Kei AU - Wakamatsu K AD - Tsukuba Daigaku. FAU - Ho, C Kiong AU - Ho CK AUID- ORCID: 0000-0002-4652-2264 AD - Tsukuba Daigaku; kiongho@md.tsukuba.ac.jp. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - RNA JT - RNA (New York, N.Y.) JID - 9509184 SB - IM OTO - NOTNLM OT - Heat-shock response OT - Post-transcriptional gene regulation OT - Trypanosoma OT - mRNA capping EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:52 PHST- 2026/03/11 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:52 [entrez] AID - rna.081035.126 [pii] AID - 10.1261/rna.081035.126 [doi] PST - aheadofprint SO - RNA. 2026 Oct 29:rna.081035.126. doi: 10.1261/rna.081035.126. PMID- 42957188 OWN - NLM STAT- Publisher LR - 20261029 IS - 1469-9001 (Electronic) IS - 1355-8382 (Linking) DP - 2026 Oct 29 TI - Antisense Oligonucleotides with 5'-decoy technology triggers nonsense-mediated decay of SCN9A to elicit long-lasting analgesia. LID - rna.081254.126 [pii] LID - 10.1261/rna.081254.126 [doi] AB - The voltage-gated sodium channel NaV1.7 (SCN9A) is a genetically validated target for pain therapy, but achieving therapeutic isoform selectivity remains a formidable challenge for small-molecule inhibitors. Here, we overcome this bottleneck through a highly selective RNA-targeted strategy that dictates SCN9A alternative splicing. We first identified ASO50 as the most potent baseline candidate antisense oligonucleotide (ASO). Subsequent 5'-decoy (5D) sequence screening yielded an upgraded candidate, L9C-ASO50, which is engineered to competitively intercept U1 snRNA, achieving a cooperative spatial hindrance that robustly suppresses exon 5 recognition. This targeted exon exclusion introduces a frameshift that triggers robust, obligate transcript clearance via the nonsense-mediated mRNA decay (NMD) pathway, ensuring complete functional silencing without cross-reacting with homologous NaV channels. Mechanistically, we define the underlying cis-trans regulatory architecture, revealing a homeostatic tug-of-war where the RNA-binding protein ELAVL2 enforces exon inclusion, paradoxically antagonized by the canonical SR protein family. Crucially, CNS-restricted delivery of L9C-ASO50 produces profound and sustained mechanical and thermal analgesia. These findings establish splice-modulated transcript degradation as a viable therapeutic paradigm for chronic pain and provide a broadly applicable blueprint for engineering targeted RNA therapeutics. CI - Published by Cold Spring Harbor Laboratory Press for the RNA Society. FAU - Meng, Shaodong AU - Meng S AUID- ORCID: 0000-0001-9661-2754 AD - Nanjing Normal University. FAU - Yang, Xinyi AU - Yang X AD - Nanjing Normal University. FAU - Liu, Xuan AU - Liu X AD - Nanjing Normal University. FAU - Li, Shangqing AU - Li S AD - Nanjing Normal University. FAU - Ma, Yongkun AU - Ma Y AD - Nanjing Normal University. FAU - Wang, Xinli AU - Wang X AD - Nanjing Normal University. FAU - Zhang, Haoyan AU - Zhang H AD - Nanjing Normal University. FAU - Chen, Rui AU - Chen R AD - Nanjing Normal University. FAU - Azhar, Zeb AU - Azhar Z AD - Nanjing Normal University. FAU - Guo, Yafei AU - Guo Y AD - Nanjing Normal University. FAU - Fu, Qingyue AU - Fu Q AD - Nanjing Normal University fuqingyue@nnu.edu.cn. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - RNA JT - RNA (New York, N.Y.) JID - 9509184 SB - IM OTO - NOTNLM OT - ASO OT - NMD OT - SCN9A OT - alternative splicing OT - analgesia EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:52 PHST- 2026/08/02 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:52 [entrez] AID - rna.081254.126 [pii] AID - 10.1261/rna.081254.126 [doi] PST - aheadofprint SO - RNA. 2026 Oct 29:rna.081254.126. doi: 10.1261/rna.081254.126. PMID- 42957183 OWN - NLM STAT- Publisher LR - 20261029 IS - 1423-0410 (Electronic) IS - 0042-9007 (Linking) DP - 2026 Oct 29 TI - From appointments to inventory: Predicting amount of daily stocked blood by blood type. LID - 10.1111/vox.70406 [doi] AB - BACKGROUND AND OBJECTIVES: Maintaining adequate blood inventories requires reliable short-term estimates of available blood units, yet daily availability varies due to calendar effects, donor behavior, and operational constraints. This study presents an interpretable computational pipeline for predicting daily stocked amounts of whole-blood units across major ABO/Rh blood types in a centralized transfusion center in Czechia. MATERIALS AND METHODS: We employed linear regression with automated forward selection and backward elimination to identify statistically significant predictors. Models were trained on retrospective daily data from 2021 to 2024, with 2025 reserved as an independent test set. Features included school holidays, proximity to non-working days, weekday and month indicators, national influenza surveillance variables, and counts of scheduled donors from two hospitals. Performance was evaluated using normalized mean absolute error (nMAE) and compared with a naive baseline based solely on scheduled donations. RESULTS: Across blood types, nMAE ranged from 6.49% (A+) to 26.26% (AB-). The proposed models consistently outperformed the naive baseline and achieved performance comparable to other machine learning approaches. Scheduled donations were the dominant predictors, with coefficients close to one, while weekday and holiday variables provided additional improvements. Influenza indicators showed weak but statistically significant associations. CONCLUSION: The proposed interpretable modeling approach provides accurate short-term predictions of blood inventory levels. Incorporating calendar and epidemiological variables offers modest improvements beyond scheduled donations, supporting more effective blood supply management. CI - (c) 2026 The Author(s). Vox Sanguinis published by John Wiley & Sons Ltd on behalf of International Society of Blood Transfusion. FAU - Dankova, Martina AU - Dankova M AUID- ORCID: 0009-0009-2603-2389 AD - Institute of Scientific Instruments of the CAS, Brno, Czech Republic. FAU - Koskova, Stanislava AU - Koskova S AUID- ORCID: 0009-0000-9570-848X AD - Transfusion and Tissue Department, The University Hospital Brno, Brno, Czech Republic. FAU - Lejdarova, Hana AU - Lejdarova H AUID- ORCID: 0000-0003-1485-2100 AD - Transfusion and Tissue Department, The University Hospital Brno, Brno, Czech Republic. FAU - Plesinger, Filip AU - Plesinger F AUID- ORCID: 0000-0003-2875-3541 AD - Institute of Scientific Instruments of the CAS, Brno, Czech Republic. LA - eng GR - RVO:68081731/Akademie Ved Ceske Republiky/ PT - Journal Article DEP - 20261029 PL - England TA - Vox Sang JT - Vox sanguinis JID - 0413606 SB - IM OTO - NOTNLM OT - blood stock prediction OT - blood supply management OT - healthcare logistics OT - interpretable models OT - operational forecasting EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:43 PHST- 2026/10/09 00:00 [revised] PHST- 2026/04/14 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:43 [entrez] AID - 10.1111/vox.70406 [doi] PST - aheadofprint SO - Vox Sang. 2026 Oct 29. doi: 10.1111/vox.70406. PMID- 42957165 OWN - NLM STAT- Publisher LR - 20261029 IS - 1538-7151 (Electronic) IS - 0277-1691 (Linking) DP - 2026 Oct 29 TI - Reproducibility of Lymphovascular Space Invasion Assessment in Cervical Squamous Cell Carcinoma: Does Experience Matter? LID - 10.1097/PGP.0000000000001213 [doi] AB - Lymphovascular space invasion (LVSI) in cervical squamous cell carcinoma is associated with increased nodal metastasis and disease recurrence, and this parameter is incorporated into clinical risk assessment and management algorithms. Despite this, there is limited data regarding pathologist reproducibility for LVSI diagnosis in cervical carcinoma. Recently, we assessed interobserver agreement for LVSI diagnosis using digital whole slide images of early-stage cervical squamous cell carcinomas with a mixture of presumed true LVSI (n=20) and LVSI mimics (n=20). Ten experienced pathologists with an average of 24 years of independent reporting experience achieved substantial agreement (kappa value=0.76), with an overall percent agreement (OPA) of 88%. Herein, we evaluate the impact of years of experience on diagnostic reproducibility of LVSI assessment using the same study set of 40 whole slide images. Fourteen participants, comprising a mix of pathology trainees (n=6) and early-career pathologists (n=8), the latter with an average of 1.25 years of independent reporting experience, achieved borderline substantial agreement (kappa value = 0.64) and an OPA of 82.5% (trainee OPA 78% and kappa= 0.55; early-career pathologist OPA 86% and kappa=0.72). Although diagnostic concordance for LVSI assessment was lower among trainees and early-career pathologists compared with experienced pathologists, the finding lacked statistical significance. This study provides evidence that trainees and early-career pathologists can recognize LVSI with a relatively high degree of accuracy, even when evaluating challenging cases in a digital setting. CI - Copyright (c) 2026 by the International Society of Gynecological Pathologists. FAU - Talia, Karen L AU - Talia KL AD - Department of Pathology, The Royal Women's and Royal Children's Hospital, Melbourne, Victoria, Australia. FAU - Soong, T Rinda AU - Soong TR AD - Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania. FAU - Kiyokawa, Takako AU - Kiyokawa T AD - Department of Pathology, The Jikei University School of Medicine, Tokyo, Japan. FAU - Bhardwaj, Swati AU - Bhardwaj S AD - Department of Pathology, Cedars Sinai Medical Center, Los Angeles, California. FAU - Beleaua, Marius-Alexandru AU - Beleaua MA AD - Department of Pathology, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, Targu Mures, Romania. AD - Department of Pathology, Bistrita Clinical County Emergency Hospital, Bistrita, Romania. FAU - Bui, Quang Hiep AU - Bui QH AD - Department of Pathology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic. FAU - Jones, Terri E AU - Jones TE AD - Department of Pathology, Magee Women's Hospital, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania. FAU - Khan, Sarah E J AU - Khan SEJ AD - Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada. FAU - Krausey, Asher AU - Krausey A AD - Department of Pathology, University of the Witwatersrand Department of Anatomical Pathology/National Health Laboratory Service, Johannesburg, South Africa. FAU - Lau, Hiu Yeung AU - Lau HY AD - Department of Pathology and Laboratory Medicine, KK Women's and Children's Hospital, Singapore. FAU - Muller, Julia AU - Muller J AD - Department of Pathology, Institute of Pathology, University Hospital, Leipzig, Germany. FAU - Safanda, Adam AU - Safanda A AD - Department of Pathology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic. FAU - Shi, Wangpan Jackson AU - Shi WJ AD - Department of Pathology, University of California, San Diego, California. FAU - Scully, Niall AU - Scully N AD - Department of Pathology, University Hospitals Plymouth NHS Trust, Plymouth, UK. FAU - Thomas, Amitha AU - Thomas A AD - Department of Pathology, Dorevitch Pathology, Frankston, Melbourne, Victoria, Australia. FAU - Ukai, Natsuko AU - Ukai N AD - Department of Pathology, The Jikei University School of Medicine, Tokyo, Japan. FAU - Wang, Joan AU - Wang J AD - Department of Pathology, St Vincent's Hospital, Melbourne, Australia. FAU - Banet, Natalie AU - Banet N AD - Department of Pathology, Dartmouth Hitchcock Medical Center, Lebanon, New Hampshire. FAU - Stolnicu, Simona AU - Stolnicu S AD - Department of Pathology, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, Targu Mures, Romania. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Int J Gynecol Pathol JT - International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists JID - 8214845 SB - IM OTO - NOTNLM OT - Cervical squamous cell carcinoma OT - Digital pathology OT - Early-career pathologist OT - Lymphovascular space invasion OT - Pathology trainee OT - Reproducibility OT - Whole slide images COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:35 PHST- 2026/06/30 00:00 [received] PHST- 2026/08/05 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:35 [entrez] AID - 00004347-990000000-00350 [pii] AID - 10.1097/PGP.0000000000001213 [doi] PST - aheadofprint SO - Int J Gynecol Pathol. 2026 Oct 29. doi: 10.1097/PGP.0000000000001213. PMID- 42957163 OWN - NLM STAT- Publisher LR - 20261029 IS - 1538-7151 (Electronic) IS - 0277-1691 (Linking) DP - 2026 Oct 28 TI - Histopathological and Clinical Perspectives on Prognostic Markers in Endometrial Cancer. LID - 10.1097/PGP.0000000000001224 [doi] AB - The microcystic, elongated, and fragmented (MELF) invasion pattern, tumor budding (TB), and tumor-infiltrating lymphocytes (TILs) may have prognostic value in endometrial carcinoma; however, their relationships are not fully defined. This retrospective study included 216 endometrioid endometrial carcinomas. MELF pattern, TB (BD1-3), and TIL levels were evaluated on hematoxylin-eosin sections. Clinicopathologic variables and immunohistochemical markers (HER2, p53, mismatch repair proteins) were analyzed statistically, including survival analysis.MELF pattern of invasion (present in 32.4%) was significantly associated with larger tumor size, low-to-intermediate grade, deep myometrial invasion, lymphovascular invasion (LVI), and nodal metastasis; on multivariate analysis, it remained independently associated with LVI (odds ratio=38.15) and nodal metastasis (odds ratio=12.82). TB was significantly associated with high-grade, deep invasion, cervical involvement, advanced FIGO stage, and mortality after Bonferroni correction; associations with tumor size, LVI, and nodal metastasis were significant only at the conventional threshold and considered trends. TB did not retain independent prognostic significance in the Cox regression model. TIL showed no significant association with any parameter, survival, or the other 2 patterns. MELF pattern of invasion is a clinically important marker independently associated with LVI and nodal metastasis. Notably, MELF was more common in low-to-intermediate grade tumors, suggesting that it may identify aggressive invasive behavior in tumors otherwise considered lower risk by grade alone. TB reflects aggressive tumor behavior without independent survival significance. TIL had limited prognostic value by routine assessment. Combined evaluation of MELF and TB may improve risk stratification in endometrioid endometrial carcinoma, particularly where molecular profiling is unavailable. CI - Copyright (c) 2026 by the International Society of Gynecological Pathologists. FAU - Aslan, Figen AU - Aslan F AUID- ORCID: 0000-0002-4817-1904 AD - Department of Pathology. FAU - Kurtulus, Gozde AU - Kurtulus G AD - Department of Pathology. FAU - Acikalin, Demet AU - Acikalin D AD - Department of Pathology. FAU - Kacar, Hulya AU - Kacar H AUID- ORCID: 0000-0001-5353-0564 AD - Department of Gynecologic Oncology, Eskisehir City Hospital, Eskisehir. FAU - Kavak, Duygu AU - Kavak D AUID- ORCID: 0000-0002-9977-6567 AD - Department of Pathology, Canakkale Mehmet Akif Ersoy State Hospital, Canakkale, Turkiye. FAU - Keskin, Nadi AU - Keskin N AUID- ORCID: 0000-0001-7097-7106 AD - Department of Pathology, Canakkale Mehmet Akif Ersoy State Hospital, Canakkale, Turkiye. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Int J Gynecol Pathol JT - International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists JID - 8214845 SB - IM OTO - NOTNLM OT - Endometrioid endometrial carcinoma OT - Lymphovascular invasion OT - MELF invasion pattern OT - Survival OT - Tumor budding OT - Tumor-infiltrating lymphocytes COIS- The authors declare no conflict of interest. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:34 PHST- 2026/05/10 00:00 [received] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:34 [entrez] AID - 00004347-990000000-00349 [pii] AID - 10.1097/PGP.0000000000001224 [doi] PST - aheadofprint SO - Int J Gynecol Pathol. 2026 Oct 28. doi: 10.1097/PGP.0000000000001224. PMID- 42957159 OWN - NLM STAT- Publisher LR - 20261029 IS - 1876-4320 (Electronic) IS - 1874-9399 (Linking) DP - 2026 Oct 29 TI - Deletion of EZH2 increases oral epithelial cell stress responses. PG - 195189 LID - S1874-9399(26)00055-6 [pii] LID - 10.1016/j.bbagrm.2026.195189 [doi] AB - Enhancer of zeste homolog 2 (EZH2), a member of polycomb repressive complex 2 (PRC2), is expressed in almost all basal stem cells (SCs) of the epithelial lining of the tongue. We previously generated a transgenic mouse model (KcEZH2) containing a tamoxifen (tam) dependent CreER system to selectively knock out (KO) EZH2 (KcEZH2-T) in most tongue basal epithelial SCs. Here, we used this model to assess EZH2 actions in normal tongue epithelia. Genome-wide transcriptomics revealed that signaling pathways associated with stress responses were increased, whereas cell-cycle-related processes and cell proliferation were decreased in KcEZH2-T oral epithelia. Indeed, EZH2 KO increased the expression of EGR1, BTG2, c-Fos, and c-Jun proteins, which are products of immediate-early genes (IEGs) associated with cellular responses during various types of cell stress, and decreased Ki67 protein. Our chromatin immunoprecipitation (ChIP) data in tongue epithelia of wild type mice showed that the chromatin near specific regions of the Egr1, Btg2, and Fos genes displayed H3K27me3 marks, the epigenetic modification catalyzed by EZH2 in canonical PRC2 complex assemblies. We additionally found that Loricrin and E-cadherin proteins were increased in KcEZH2-T vs. KcEZH2 oral epithelia. Thus, our data demonstrate that deletion of EZH2 increases stress-related responses in normal tongue epithelia. As previous studies show that IEGs are increased while cell cycle progression is suppressed in early stages of wound repair, transiently inhibiting EZH2 expression and function warrants further investigation as a hypothetical framework for innovative wound healing approaches. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Baquero, Jorge AU - Baquero J AD - Department of Pharmacology, Weill Cornell Medical College, New York, NY, USA; Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA. FAU - Tang, Xiao-Han AU - Tang XH AD - Department of Pharmacology, Weill Cornell Medical College, New York, NY, USA; Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA. FAU - Zhang, Tuo AU - Zhang T AD - Weill Cornell Genomics Core Facility, Weill Cornell Medical College, New York, NY, USA. FAU - Del Aguila, Eduardo Mere AU - Del Aguila EM AD - Department of Pharmacology, Weill Cornell Medical College, New York, NY, USA; Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA. FAU - Gudas, Lorraine J AU - Gudas LJ AD - Department of Pharmacology, Weill Cornell Medical College, New York, NY, USA; Meyer Cancer Center, Weill Cornell Medicine, New York, NY, USA; Department of Pharmacology, Weill Cornell Graduate School of Biomedical Sciences, New York, NY, USA. Electronic address: ljgudas@med.cornell.edu. LA - eng PT - Journal Article DEP - 20261029 PL - Netherlands TA - Biochim Biophys Acta Gene Regul Mech JT - Biochimica et biophysica acta. Gene regulatory mechanisms JID - 101731723 SB - IM OTO - NOTNLM OT - AP-1 complex OT - BTG2 OT - EGR1 OT - Epigenetics OT - Oral epithelial stem cells OT - Transcriptional regulation OT - c-Fos COIS- Declaration of competing interest The authors declare no competing interests. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:34 PHST- 2026/06/22 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:34 [entrez] AID - S1874-9399(26)00055-6 [pii] AID - 10.1016/j.bbagrm.2026.195189 [doi] PST - aheadofprint SO - Biochim Biophys Acta Gene Regul Mech. 2026 Oct 29:195189. doi: 10.1016/j.bbagrm.2026.195189. PMID- 42957155 OWN - NLM STAT- Publisher LR - 20261029 IS - 1096-0805 (Electronic) IS - 0022-2011 (Linking) DP - 2026 Oct 29 TI - Hepatospora eriocheir infection affects the fitness of adult Chinese mitten crabs in their non-native range. PG - 108784 LID - S0022-2011(26)00260-0 [pii] LID - 10.1016/j.jip.2026.108784 [doi] AB - For many invasive crustaceans, including the Chinese mitten crab (Eriocheir sinensis), the prevalence and impact of parasites in their introduced range remain largely unexplored. The microsporidium Hepatospora eriocheir was recently detected in mitten crabs across Europe, yet little is known about its physiological effects on its host. In this study, the influence of H. eriocheir infection on physiological biomarkers in adult mitten crabs collected from rivers in Germany and Belgium was experimentally investigated. We assessed metabolic rates (routine and maximum metabolic rate, aerobic scope), haemolymph glucose and lactate levels, and lipid content in the hepatopancreas. Infection prevalence reached 27% in adult mitten crabs from the Belgian population and 38% in individuals from the German population. Infected individuals showed a significantly reduced maximum metabolic rate and aerobic scope, while the routine metabolic rate remained unaffected. Glucose levels were lower in infected crabs both at rest and post-exercise, which indicates that energy reserves have dropped, whereas lactate levels showed no significant difference. The lipid content in the hepatopancreas was reduced, likely due to direct exploitation of host resources by the parasite and damage to hepatopancreatic tissue. Together, these patterns indicate an impaired energy metabolism, which may hamper energetically demanding activities such as migration and reproduction. The Chinese mitten crab is a catadromous species, and as the examined crabs were at the life stage when they commence a long-distance migration for their single reproductive event, infection by H. eriocheir may compromise their ability to complete this migration and successfully reproduce. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Keirsebelik, Heleen AU - Keirsebelik H AD - University of Antwerp, Department of Biology, ECOSPHERE Research Group, Universiteitsplein 1C, 2610 Wilrijk, Belgium. Electronic address: heleen.keirsebelik@uantwerpen.be. FAU - Ewers, Christine AU - Ewers C AD - Leibniz Institute for the Analysis of Biodiversity Change, Bundesstrasse 55, 20246 Hamburg, Germany. Electronic address: c.ewers@leibniz-lib.de. FAU - Opinion, April Grace AU - Opinion AG AD - University of Antwerp, Department of Biology, ECOSPHERE Research Group, Universiteitsplein 1C, 2610 Wilrijk, Belgium. Electronic address: aprilgrace.opinion@uantwerpen.be. FAU - Stachnik, Magdalena AU - Stachnik M AD - National Veterinary Research Institute, Department of Parasitology and Invasive Diseases, Bee Diseases and Aquatic Animal Disease, Al. Partyzantow 57, 24-100 Pulawy, Poland. Electronic address: magdalena.stachnik@piwet.pulawy.pl. FAU - Normant-Saremba, Monika AU - Normant-Saremba M AD - University of Gdansk, Faculty of Oceanography and Geography, Department of Marine Ecology, Al. M. Pilsudskiego 46, 81-378 Gdynia, Poland. Electronic address: monika.normant@ug.edu.pl. FAU - Poma, Giulia AU - Poma G AD - University of Antwerp, Department of Pharmaceutical Sciences, Toxicological Centre, Universiteitsplein 1S, 2610 Wilrijk, Belgium. Electronic address: giulia.poma@uantwerpen.be. FAU - Bosschaerts, Stijn AU - Bosschaerts S AD - University of Antwerp, Department of Pharmaceutical Sciences, Toxicological Centre, Universiteitsplein 1S, 2610 Wilrijk, Belgium. Electronic address: Stijn.Bosschaerts@uantwerpen.be. FAU - De Boeck, Gudrun AU - De Boeck G AD - University of Antwerp, Department of Biology, ECOSPHERE Research Group, Universiteitsplein 1C, 2610 Wilrijk, Belgium. Electronic address: gudrun.deboeck@uantwerpen.be. FAU - Schoelynck, Jonas AU - Schoelynck J AD - University of Antwerp, Department of Biology, ECOSPHERE Research Group, Universiteitsplein 1C, 2610 Wilrijk, Belgium. Electronic address: jonas.schoelynck@uantwerpen.be. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Invertebr Pathol JT - Journal of invertebrate pathology JID - 0014067 SB - IM OTO - NOTNLM OT - Biomarkers OT - Crustacea OT - Decapod OT - Eriocheir sinensis OT - Invasive species OT - Microsporidium OT - Parasite COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:34 PHST- 2026/06/02 00:00 [received] PHST- 2026/08/13 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:34 [entrez] AID - S0022-2011(26)00260-0 [pii] AID - 10.1016/j.jip.2026.108784 [doi] PST - aheadofprint SO - J Invertebr Pathol. 2026 Oct 29:108784. doi: 10.1016/j.jip.2026.108784. PMID- 42957134 OWN - NLM STAT- Publisher LR - 20261029 IS - 1532-2939 (Electronic) IS - 0195-6701 (Linking) DP - 2026 Oct 29 TI - Enterococcus spp. dry surface biofilm resilience to disinfection. LID - S0195-6701(26)00425-1 [pii] LID - 10.1016/j.jhin.2026.10.022 [doi] AB - BACKGROUND: Dry surface biofilms (DSB) are increasingly recognised as reservoirs of healthcare-associated pathogens that contribute to environmental persistence and transmission. However, the susceptibility of enterococcal DSB to disinfectants remains poorly understood. AIM: To evaluate the efficacy of commonly used disinfectants against enterococcal DSB and identify best practices for their removal. METHODS: The efficacy of benzalkonium chloride (BZC), sodium dichloroisocyanurate (NaDCC), sodium hypochlorite (NaOCl), a complex cationic formulation, and two peracetic acid (PAA)-based formulations was assessed against hydrated and dry surface biofilms of clinical and reference strains of enterococci in standard efficacy test procedures. The effects of disinfectant concentration, contact time, mechanical wiping, biofilm format, and surface material were also investigated. FINDINGS: Most BZC and NaDCC treatments failed to achieve the >/=4 Log(10) CFU/mL reduction, whereas selected PAA formulations consistently met this threshold. Increasing PAA concentration and contact time significantly improved efficacy. Mechanical wiping enhanced NaOCl performance, increasing bacterial reduction by a mean of 1.61 Log(10) CFU/mL, while a commercially available disinfectant wipe achieved a mean reduction of 5.31 Log(10) CFU/mL. Susceptibility varied with disinfectant chemistry and with the type of surface materials. Hydrated biofilms were less susceptible than DSB following BZC treatment, whereas no significant differences were observed with most PAA treatments. Likewise, BZC and NaDCC efficacy was generally lower against DSB on stainless steel compared to polycotton or polyester, but not following PAA treatment. CONCLUSION: Enterococcal DSB exhibit substantial tolerance to several commonly used disinfectants, while selected PAA formulations consistently achieved accepted efficacy thresholds. Mechanical wiping significantly improved disinfectant performance, supporting the combined use of oxidising disinfectants and physical biofilm disruption for effective surface decontamination. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Harsent, R AU - Harsent R AD - School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, UK. FAU - Cattoir, V AU - Cattoir V AD - Service de Bacteriologie-Hygiene Hospitaliere, CHU de Rennes, Rennes, France; CNR de la Resistance aux Antibiotiques (laboratoire associe'Enterocoques'), CHU de Rennes, Rennes, France; Unite Inserm U1230 BRM, Universite de Rennes, Rennes, France. FAU - Pascoe, M J AU - Pascoe MJ AD - School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, UK. Electronic address: maillardj@cardiff.ac.uk. FAU - Pertusati, F AU - Pertusati F AD - School of Chemistry, Cardiff University, Cardiff, UK. FAU - Maillard, J-Y AU - Maillard JY AD - School of Pharmacy and Pharmaceutical Sciences, Cardiff University, Cardiff, UK. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - J Hosp Infect JT - The Journal of hospital infection JID - 8007166 SB - IM OTO - NOTNLM OT - Dry surface biofilm OT - disinfectant tolerance OT - enterococci OT - environmental persistence COIS- Conflict of Interest None to declare EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:34 PHST- 2026/07/17 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:34 [entrez] AID - S0195-6701(26)00425-1 [pii] AID - 10.1016/j.jhin.2026.10.022 [doi] PST - aheadofprint SO - J Hosp Infect. 2026 Oct 29:S0195-6701(26)00425-1. doi: 10.1016/j.jhin.2026.10.022. PMID- 42957127 OWN - NLM STAT- Publisher LR - 20261029 IS - 1535-7732 (Electronic) IS - 1051-0443 (Linking) DP - 2026 Oct 29 TI - Percutaneous Recanalization of Chronic Noncirrhotic Native-Liver Extrahepatic Portal Vein Obstruction in Children. PG - 109174 LID - S1051-0443(26)01185-1 [pii] LID - 10.1016/j.jvir.2026.109174 [doi] AB - PURPOSE: To evaluate technical success, procedure-related safety, and imaging-based patency of percutaneous portal vein recanalization (PVR) in children with chronic native-liver extrahepatic portal vein obstruction (EHPVO). MATERIALS AND METHODS: This retrospective single-center study included 28 consecutive patients younger than 17 years with chronic noncirrhotic, nonmalignant EHPVO who underwent attempted PVR between 2016 and 2026. Recanalization was performed through transhepatic, transsplenic, or combined access, followed by balloon angioplasty without stenting. Technical success was defined as restoration of hepatopetal flow through the portal vein into the intrahepatic portal venous system. Adverse events were graded using the Society of Interventional Radiology classification. Portal vein patency was assessed on available follow-up imaging. RESULTS: Technical success was achieved in 19 of 28 patients (67.9%). Six patients (21.4%) had access-related adverse events (3 mild, 1 moderate, 2 severe), with no surgery, permanent sequelae, or procedure-related death. Follow-up imaging was available for 15 of 19 technically successful patients, with a median imaging follow-up of 11 months (range, 1-35 months). At last imaging, the portal vein was patent without significant restenosis in 7 patients, patent with restenosis in 5, and reoccluded in 3. Overall, portal venous flow was maintained in 12 of 15 patients (80.0%). CONCLUSION: Percutaneous PVR restored hepatopetal portal flow in 67.9% of selected children without stenting. Durable patency could not be established because follow-up was incomplete and heterogeneous. Restenosis and reocclusion support structured imaging surveillance; further studies should define the role of stenting versus angioplasty alone. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Bayramoglu, Mert AU - Bayramoglu M AD - Department of Radiology, Koc University Hospital, Istanbul, Turkiye. Electronic address: mbayramoglu@kuh.ku.edu.tr. FAU - Canturk, Enes Muhammed AU - Canturk EM AD - Department of Radiology, Koc University Hospital, Istanbul, Turkiye. FAU - Samadli, Vugar AU - Samadli V AD - Department of Radiology, Koc University Hospital, Istanbul, Turkiye. FAU - Veznikli, Mert AU - Veznikli M AD - Department of Biostatistics, Koc University School of Medicine, Istanbul, Turkiye. FAU - Uslu Kizilkan, Nuray AU - Uslu Kizilkan N AD - Division of Pediatric Gastroenterology, Hepatology and Nutrition, Department of Pediatrics, Koc University School of Medicine, Istanbul, Turkiye. FAU - Arikan, Cigdem AU - Arikan C AD - Division of Pediatric Gastroenterology, Hepatology and Nutrition, Department of Pediatrics, Koc University School of Medicine, Istanbul, Turkiye. FAU - Oguzkurt, Levent AU - Oguzkurt L AD - Department of Radiology, Koc University Hospital, Istanbul, Turkiye. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Vasc Interv Radiol JT - Journal of vascular and interventional radiology : JVIR JID - 9203369 SB - IM OTO - NOTNLM OT - Children OT - balloon angioplasty OT - extrahepatic portal vein obstruction OT - portal hypertension OT - portal vein recanalization EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:34 PHST- 2026/08/17 00:00 [received] PHST- 2026/10/22 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:34 [entrez] AID - S1051-0443(26)01185-1 [pii] AID - 10.1016/j.jvir.2026.109174 [doi] PST - aheadofprint SO - J Vasc Interv Radiol. 2026 Oct 29:109174. doi: 10.1016/j.jvir.2026.109174. PMID- 42957126 OWN - NLM STAT- Publisher LR - 20261029 IS - 1535-7732 (Electronic) IS - 1051-0443 (Linking) DP - 2026 Oct 29 TI - A Branch-Specific Combined Fenestration Strategy for Chronic Aortic Dissection With Severe Paravisceral True Lumen Compression. PG - 109175 LID - S1051-0443(26)01186-3 [pii] LID - 10.1016/j.jvir.2026.109175 [doi] AB - PURPOSE: To evaluate a branch-specific combined fenestration strategy to address the technical challenges of severe true lumen (TL) compression during physician-modified fenestration (PMF)-based four-vessel visceral reconstruction in chronic aortic dissection (CAD). MATERIALS AND METHODS: This retrospective study enrolled twenty-three consecutive patients with aneurysmal CAD and severe paravisceral TL compression (minimum functional TL diameter /=20 years in 2023, stratified by sex and age group. We additionally examined age-standardized global prevalence trends for obesity and selected diseases associated with excess adiposity, including type 2 diabetes mellitus (T2DM), metabolic dysfunction-associated steatotic liver disease (MASLD), chronic kidney disease (CKD), cardiovascular disease (CVD), along with other chronic diseases associated with excess adiposity, such as dementias and obesity-related cancers, over 2000-2023.In 2023, the prevalence of overweight was generally higher in men, whereas obesity was more prevalent among women, particularly during middle age. Both overweight and obesity increased from early adulthood, peaked during middle age, and subsequently declined at older ages. Globally, obesity prevalence increased by approximately 95% between 2000 and 2023, reaching 19.5% in 2023. During the same period, the age-standardized prevalence of MASLD, T2DM, CKD, dementias, and obesity-related cancers increased. In contrast, age-standardized CVD prevalence decreased slightly and the divergent trends between obesity and age-standardized CVD prevalence highlight the multifactorial nature of cardiovascular risk and the potential impact of improvements in other cardiovascular risk factors. These findings underscore the urgent need for integrated, population-level strategies to prevent and effectively manage obesity and its complications. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Boutari, Chrysoula AU - Boutari C AD - Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, 02215, USA; Fourth Department of Internal Medicine, Hippokratio Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece. Electronic address: chrisoulabgr@yahoo.gr. FAU - Mantzoros, Christos S AU - Mantzoros CS AD - Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, 02215, USA; Department of Medicine, Boston VA Healthcare System, Harvard Medical School, Boston, MA, 02215, USA. LA - eng PT - Editorial DEP - 20261029 PL - United States TA - Metabolism JT - Metabolism: clinical and experimental JID - 0375267 SB - IM OTO - NOTNLM OT - BMI OT - Cancers OT - Cardiovascular disease OT - Chronic kidney disease OT - Dementias OT - Diabetes OT - MASLD OT - Obesity OT - Overweight OT - Prevalence COIS- Declaration of competing interest CB declares no conflicts of interest. CSM reports grants through his institution from Merck, Massachusetts Life Sciences Center, Abbott, Amgen and Boehringer Ingelheim, he reports personal consulting fees and support through his institution from Ansh Inc., collaborative research support from LabCorp Inc., reports personal consulting fees from Nestle, Olympus, Genfit, Lumos, Novo Nordisk, Amgen, Corcept, Intercept, 89 Bio, Madrigal, Aligos, Esperion, Biodexa, Verdiva Bio, Boehringer Ingelheim, Abbott and Regeneron, reports educational activity meals through his institution or national conferences from Esperion, Merck, Boehringer Ingelheim and travel support and fees from UptoDate, TMIOA, Elsevier, MedScape and the Cardio Metabolic Health Conference and shares with Coherus Oncology. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:31 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/22 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:31 [entrez] AID - S0026-0495(26)00325-2 [pii] AID - 10.1016/j.metabol.2026.156812 [doi] PST - aheadofprint SO - Metabolism. 2026 Oct 29:156812. doi: 10.1016/j.metabol.2026.156812. PMID- 42956987 OWN - NLM STAT- Publisher LR - 20261029 IS - 1872-7980 (Electronic) IS - 0304-3835 (Linking) DP - 2026 Oct 29 TI - Acquisition of Therapeutic Resistance and Structured Tissue Differentiation via Activation of the Life Cycle of Polyploid Giant Cancer Cells. PG - 218928 LID - S0304-3835(26)00692-0 [pii] LID - 10.1016/j.canlet.2026.218928 [doi] AB - To elucidate the mechanisms underlying therapy-induced resistance, we investigated the temporal dynamics of the polyploid giant cancer cell (PGCC) life cycle following treatment with the microtubule-destabilizing agent vincristine (VCR). Longitudinal live-cell fluorescence imaging revealed that VCR-induced mitotic suppression activated a distinct PGCC life cycle characterized by nuclear amplification and progressive cellular reprogramming. At the cellular level, PGCCs entered a senescence-like state accompanied by an enhanced senescence-associated secretory phenotype (SASP), increased epithelial-mesenchymal transition (EMT), acquisition of early embryonic-like features, and multilineage differentiation potential into ectoderm, mesoderm, and endoderm. PGCC-derived progeny exhibited dose- and time-dependent changes in proliferative behavior, aggressive phenotypes in vitro, along with time-dependent tumorigenic potential in vivo. Remarkably, at the tissue level, PGCCs also underwent spatial nuclear reorganization and self-organization into morphologically defined glandular epithelial structures exhibiting varying degrees of tissue maturation, reminiscent of early embryonic development. Silencing IL1beta, IL6, or IL8, or pharmacologically inhibiting their receptors, suppressed PGCC formation, budding, EMT, and embryonic-like reprogramming associated with tissue morphogenesis. Furthermore, NAMPT emerged as a key regulator of embryonic-like stemness, glandular morphogenesis, and structural expansion. Together, these findings reveal that therapeutic stress activates a PGCC life cycle capable of generating two divergent outcomes: resistant tumor regeneration through clonal selection of undifferentiated tumor cells and organized tissue differentiation through spatial nuclear reprogramming. Our study establishes a conceptual framework linking therapy-induced polyploidization, developmental plasticity, and resistance generation at both cellular and tissue levels. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Zhang, Zhiqian AU - Zhang Z AD - Department of Anatomic Pathology, Division of Pathology and Laboratory Medicine, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030-4095, United States; Department of Human Cell Biology and Genetics, School of Medicine, Southern University of Science and Technology, 1088 Xueyuan Blvd, Shenzhen, Guangdong 518055, China; Laboratory of Homeostatic Medicine, School of Medicine, Southern University of Science and Technology, 1088 Xueyuan Blvd, Shenzhen, Guangdong 518055, China. FAU - Li, Xiaoran AU - Li X AD - Department of Anatomic Pathology, Division of Pathology and Laboratory Medicine, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030-4095, United States. FAU - Tian, Xinxin AU - Tian X AD - Department of Human Cell Biology and Genetics, School of Medicine, Southern University of Science and Technology, 1088 Xueyuan Blvd, Shenzhen, Guangdong 518055, China; Laboratory of Homeostatic Medicine, School of Medicine, Southern University of Science and Technology, 1088 Xueyuan Blvd, Shenzhen, Guangdong 518055, China. FAU - Deng, Limin AU - Deng L AD - Department of Human Cell Biology and Genetics, School of Medicine, Southern University of Science and Technology, 1088 Xueyuan Blvd, Shenzhen, Guangdong 518055, China; Laboratory of Homeostatic Medicine, School of Medicine, Southern University of Science and Technology, 1088 Xueyuan Blvd, Shenzhen, Guangdong 518055, China. FAU - Dong, Jin-Tang AU - Dong JT AD - Department of Human Cell Biology and Genetics, School of Medicine, Southern University of Science and Technology, 1088 Xueyuan Blvd, Shenzhen, Guangdong 518055, China; Laboratory of Homeostatic Medicine, School of Medicine, Southern University of Science and Technology, 1088 Xueyuan Blvd, Shenzhen, Guangdong 518055, China. Electronic address: dongjt@sustech.edu.cn. FAU - Liu, Jinsong AU - Liu J AD - Department of Anatomic Pathology, Division of Pathology and Laboratory Medicine, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Houston, TX 77030-4095, United States. Electronic address: jliu@mdanderson.org. LA - eng PT - Journal Article DEP - 20261029 PL - Ireland TA - Cancer Lett JT - Cancer letters JID - 7600053 SB - IM OTO - NOTNLM OT - EMT OT - Glandular-like architecture OT - Life cycle OT - NAMPT OT - PGCCs OT - SASP COIS- Declaration of Competing Interest All authors declare no conflicts of interest. Regarding the last corresponding author's role as an Editorial Board Member of this journal and serving as Guest Editor for this Special Issue: to ensure impartiality, a formal declaration of recusal from the editorial review process has been made, confirming that the last corresponding author had no involvement whatsoever in the peer review or decision-making regarding this manuscript. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:31 PHST- 2026/06/14 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:31 [entrez] AID - S0304-3835(26)00692-0 [pii] AID - 10.1016/j.canlet.2026.218928 [doi] PST - aheadofprint SO - Cancer Lett. 2026 Oct 29:218928. doi: 10.1016/j.canlet.2026.218928. PMID- 42956986 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-176X (Electronic) IS - 0300-5712 (Linking) DP - 2026 Oct 29 TI - Registration accuracy, time, and osteotomy outcomes across four strategies for task-autonomous robotic maxillofacial osteotomy. PG - 107143 LID - S0300-5712(26)00813-4 [pii] LID - 10.1016/j.jdent.2026.107143 [doi] AB - OBJECTIVES: To compare four registration strategies for task-autonomous robotic maxillofacial osteotomy. METHODS: A maxillofacial model was constructed from imaging data. The maxilla and mandible were registered separately using splint-based landmark registration (SL), dental-landmark registration (DL), dental-surface registration (DS), and bone-surface registration (BS). The target-registration error (TRE) was measured in three osteotomy regions, and registration time was recorded. Osteotomies were then performed using a task-autonomous robotic system. The actual and planned osteotomy surfaces were compared using the root mean square (RMS) deviation, mean signed deviation, and angular deviation. RESULTS: All four strategies achieved mean TREs of less than 0.8 mm. In the Le Fort I region, TRE was lower for BS than for SL (0.398 +/- 0.126 vs. 0.580 +/- 0.098 mm; P = 0.0045). Mandibular TREs showed a significant strategy-by-region interaction (P = 0.0031); in the genioplasty region, DS and BS yielded lower TREs than SL and DL. In the mandibular angle region, DS yielded lower TREs than SL and DL, and BS yielded lower TREs than DL. SL had the shortest registration time for both jaws. In the genioplasty region, DL resulted in greater RMS and angular deviations than the other strategies, whereas in the mandibular angle region, DS resulted in lower angular deviation than SL and DL. CONCLUSIONS: Registration strategy significantly influenced TRE, registration time, and mandibular osteotomy accuracy outcomes. All four strategies achieved submillimetric TREs. Overall, surface-based registration (BS and DS) yielded TREs that were lower than or comparable to those of landmark-based registration (SL and DL) and showed favorable performance for selected mandibular osteotomy accuracy outcomes. However, no consistent difference in accuracy was identified between DS and BS. CLINICAL SIGNIFICANCE: Surface-based registration may support accurate robot-assisted osteotomy when adequate dentition or bony surfaces are accessible. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Song, Shiwei AU - Song S AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Digital Dentistry Center, School of Stomatology, The Fourth Military Medical University, No.145 Changle West Road, Xincheng District, Xi'an, Shaanxi 710032, China. FAU - Liu, Heng AU - Liu H AD - School of Mechanical Engineering and Automation, Beihang University, Beijing 100191, China. FAU - Liu, Chen AU - Liu C AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Digital Dentistry Center, School of Stomatology, The Fourth Military Medical University, No.145 Changle West Road, Xincheng District, Xi'an, Shaanxi 710032, China. FAU - Yan, Xueping AU - Yan X AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Digital Dentistry Center, School of Stomatology, The Fourth Military Medical University, No.145 Changle West Road, Xincheng District, Xi'an, Shaanxi 710032, China. FAU - Liu, Yuchen AU - Liu Y AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Digital Dentistry Center, School of Stomatology, The Fourth Military Medical University, No.145 Changle West Road, Xincheng District, Xi'an, Shaanxi 710032, China. FAU - Shi, Yulin AU - Shi Y AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Digital Dentistry Center, School of Stomatology, The Fourth Military Medical University, No.145 Changle West Road, Xincheng District, Xi'an, Shaanxi 710032, China.. Electronic address: shiyulin-1989@163.com. FAU - Bai, Shizhu AU - Bai S AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Digital Dentistry Center, School of Stomatology, The Fourth Military Medical University, No.145 Changle West Road, Xincheng District, Xi'an, Shaanxi 710032, China.. Electronic address: baishizhu@foxmail.com. FAU - Zhao, Yimin AU - Zhao Y AD - State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Digital Dentistry Center, School of Stomatology, The Fourth Military Medical University, No.145 Changle West Road, Xincheng District, Xi'an, Shaanxi 710032, China.. Electronic address: zhaoym@fmmu.edu.cn. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - J Dent JT - Journal of dentistry JID - 0354422 SB - IM OTO - NOTNLM OT - Image registration OT - Orthognathic surgery OT - Osteotomy OT - Robotic surgery OT - Surface registration OT - Surgical navigation OT - Target-registration error COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:31 PHST- 2026/07/02 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:31 [entrez] AID - S0300-5712(26)00813-4 [pii] AID - 10.1016/j.jdent.2026.107143 [doi] PST - aheadofprint SO - J Dent. 2026 Oct 29:107143. doi: 10.1016/j.jdent.2026.107143. PMID- 42956982 OWN - NLM STAT- Publisher LR - 20261029 IS - 1549-9642 (Electronic) IS - 1549-9634 (Linking) DP - 2026 Oct 29 TI - Fucoidan-functionalized liposomal nanocarriers for targeted miR-217-5p delivery and combined antitumor treatment in pancreatic ductal adenocarcinoma. PG - 103046 LID - S1549-9634(26)00147-4 [pii] LID - 10.1016/j.nano.2026.103046 [doi] AB - The clinical translation of microRNA (miRNA) therapy is hindered by poor tumor specificity and inefficient delivery. In this study, we constructed a targeted nanoplatform using marine sulfated polysaccharide fucoidan (FU) to encapsulate miR-217-5p in FU-modified cationic liposomes (FU-LIP-miR) for the treatment of pancreatic ductal adenocarcinoma (PDAC), alone or in combination with gemcitabine (GEM). In this system, FU serves primarily as a targeting ligand. FU was incorporated as a functional surface ligand. Previous studies have demonstrated a high-affinity interaction between FU and P-selectin, suggesting that this interaction may contribute to the enhanced tumor-associated accumulation of FU-functionalized nanocarriers. Furthermore, FU is susceptible to degradation by H(2)O(2) within the tumor microenvironment, consequently enabling controlled drug release. Initial findings confirmed that that miR-217-5p was significantly downregulated in PDAC tissues. FU-LIP-miR promoted the tumor accumulation and delivery of miR-217-5p and restored its expression, which was accompanied by reduced PDAC cell proliferation, migration, and invasion. Notably, FU can also be employed as an active targeting ligand. FU-LIP-miR combined with GEM produced an enhanced antitumor effect and increased apoptosis in PDAC cells. In a subcutaneous xenograft model using Panc02 cells in C57BL/6 mice, the combination of FU-LIP-miR and GEM produced enhanced antitumor efficacy compared with the corresponding control treatments. This study underscores the pivotal role of FU as a versatile polysaccharide in the design of targeted delivery platforms, thereby providing a novel and promising noncoding RNA-based therapeutic approach for PDAC using polysaccharide materials. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Tan, Chunlu AU - Tan C AD - Department of Pancreatic Surgery and General Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China. FAU - Fang, Yaohui AU - Fang Y AD - College of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China. FAU - Guo, Ruizhe AU - Guo R AD - Department of Biological Systems Engineering, Virginia Polytechnic Institute and State University, Blacksburg, VA, 24061, USA. FAU - Zheng, Zhenjiang AU - Zheng Z AD - Department of Pancreatic Surgery and General Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China. FAU - He, Mingrui AU - He M AD - College of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China. FAU - Yang, Jianchen AU - Yang J AD - Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX, 78712, USA. FAU - Silli, Epiphane K AU - Silli EK AD - School of Pharmacy, Department of Pharmaceutics, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China. FAU - Hu, Qiqi AU - Hu Q AD - School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China. FAU - Zheng, Huien AU - Zheng H AD - School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China. FAU - Qi, Yihui AU - Qi Y AD - School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China. FAU - Jiang, Yuqing AU - Jiang Y AD - School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China. FAU - Xing, Shubo AU - Xing S AD - School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China. FAU - Sun, Yanan AU - Sun Y AD - School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China. FAU - Tyahyo, Michelle Joycelyn AU - Tyahyo MJ AD - School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China. FAU - Li, Qian AU - Li Q AD - College of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China. Electronic address: liqian@cpu.edu.cn. FAU - Wang, Ying AU - Wang Y AD - College of Life Science and Technology, China Pharmaceutical University, Nanjing, Jiangsu, 211198, China. Electronic address: ywang@cpu.edu.cn. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Nanomedicine JT - Nanomedicine : nanotechnology, biology, and medicine JID - 101233142 SB - IM OTO - NOTNLM OT - CTNNB1 OT - Fucoidan OT - Liposome OT - PDAC OT - miR-217-5p COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:31 PHST- 2026/06/01 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:31 [entrez] AID - S1549-9634(26)00147-4 [pii] AID - 10.1016/j.nano.2026.103046 [doi] PST - aheadofprint SO - Nanomedicine. 2026 Oct 29:103046. doi: 10.1016/j.nano.2026.103046. PMID- 42956974 OWN - NLM STAT- Publisher LR - 20261029 IS - 1532-8392 (Electronic) IS - 0046-8177 (Linking) DP - 2026 Oct 29 TI - Non-Conventional Histologic Components in Urothelial Carcinoma: Post-Neoadjuvant Chemotherapy Pathologic Outcomes in a Multi-institutional Radical Cystectomy Cohort. PG - 106282 LID - S0046-8177(26)00251-0 [pii] LID - 10.1016/j.humpath.2026.106282 [doi] AB - BACKGROUND: In urothelial carcinoma (UC), the presence of many form of divergent differentiation or histologic subtypes (henceforth collectively designated as UC with non-conventional components [UC-NCC]) negatively influences disease-specific clinical outcomes when compared to typical UC. Prior studies have suggested that UC-NCC may show lower rates of response to neoadjuvant chemotherapy (NAC), a factor that may partially explain their more aggressive course. However, data on post-NAC pathology outcomes in radical cystectomy (RC) are conflicting, and the influence of the number of UC-NCC on NAC response remains unknown. In this study, we evaluated the impact of UC-NCC on post-NAC RC outcomes, accounting for the number of UC-NCC components present within individual cases. METHODS: The pathology databases of the participating institutions were queried for post-NAC RCs. Those with pathology results available from the prior transurethral resection (TURBT) were selected for inclusion in the study. Cases were separated according to the presence or absence of UC-NCC in a pre-NAC TURBT showing muscle-invasive cancer. Those with UC-NCC were further stratified according to the number of UC-NCC present (1 versus 2 versus 3 or more). The following pathology outcomes were assessed on RCs: pathologic downstaging (100 different insecticides. Resistance is usually either incompletely recessive or incompletely dominant, and inheritance of recessive traits is almost exclusively monogenic, likely contributing to their rapid expression following selection. Key resistance mechanisms are metabolic enzymes (which can confer cross-resistance between insecticides with different modes-of-action) and target site mutations. Field resistance to deltamethrin, chlorantraniliprole and indoxacarb was detected in Fiji in 2013. In 2014 a new Bacillus thuringiensis (Bt) product was incorporated into an insecticide resistance management (IRM) strategy designed to preserve its efficacy. Field resistance declined rapidly and susceptibility to all products remained high until 2018, when resistance to abamectin and chlorantraniliprole emerged. A revised IRM strategy that included previously unavailable selective insecticides resulted in declines in resistance and populations remained highly susceptible to Bt a decade after its introduction. Effective farmer and industry engagement were critical for success. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Inc. All rights reserved. FAU - Furlong, Michael J AU - Furlong MJ AD - School of the Environment, The University of Queensland, St Lucia 4072, Queensland, Australia. Electronic address: m.furlong@uq.edu.au. FAU - Nand, Nitesh AU - Nand N AD - Land Resources Division, Pacific Community, Nabua Campus, Nabua, Suva, Fiji. Electronic address: NiteshN@spc.net. FAU - Atumurirava, Fereti AU - Atumurirava F AD - Land Resources Division, Pacific Community, Nabua Campus, Nabua, Suva, Fiji. Electronic address: fatumurirava@gmail.com. FAU - Mua, Mani AU - Mua M AD - School of the Environment, The University of Queensland, St Lucia 4072, Queensland, Australia. Electronic address: mani.mua@uq.edu.au. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - Netherlands TA - Curr Opin Insect Sci JT - Current opinion in insect science JID - 101635599 SB - IM COIS- Declaration of Competing Interest None of the authors have any competing interests to declare with respect to this work. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:31 PHST- 2026/02/27 00:00 [received] PHST- 2026/06/30 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:31 [entrez] AID - S2214-5745(26)00159-8 [pii] AID - 10.1016/j.cois.2026.101643 [doi] PST - aheadofprint SO - Curr Opin Insect Sci. 2026 Oct 29:101643. doi: 10.1016/j.cois.2026.101643. PMID- 42956964 OWN - NLM STAT- Publisher LR - 20261029 IS - 1878-5875 (Electronic) IS - 1357-2725 (Linking) DP - 2026 Oct 29 TI - Structure, Function, and Role of Schlafen 5 in Cancer. PG - 107058 LID - S1357-2725(26)00168-8 [pii] LID - 10.1016/j.biocel.2026.107058 [doi] AB - Schlafen 5 (SLFN5) is a member of the Schlafen protein family originally identified for its roles in immune regulation and cell cycle control. Structurally, SLFN5 contains an N-terminal Schlafen Core domain involved in nucleic-acid binding, a Linker domain, and a C-terminal Helicase domain required for efficient suppression of LINE-1 retrotransposition. In cancer, SLFN5 exhibits highly context-dependent functions, acting either as a tumor suppressor or as a pro-tumorigenic factor depending on tumor type and experimental conditions. It suppresses proliferation and invasion in breast cancer and renal cell carcinoma via PTEN/AKT signaling and downregulation of MMPs, while promoting progression in castration-resistant prostate cancer, glioblastoma, and colorectal cancer through AR/mTORC1, STAT1 co-repression, and IFN-JAK-STAT inhibition, respectively. Discrepant findings in lung cancer suggest that supraphysiological expression may trigger non-canonical effects. Clinically, SLFN5 expression correlates with tumor stage, metastasis, and patient survival, supporting further evaluation of SLFN5 as a potential prognostic biomarker, although its diagnostic utility remains to be established. This review summarizes the structural features, molecular functions, and tumor-type-specific roles of SLFN5, with an emphasis on recent advances in oncology research, providing insights that may contribute to personalized cancer therapy and prognostic evaluation. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Gai, Keke AU - Gai K FAU - Li, Xueying AU - Li X AD - School of Health, Binzhou Polytechnic, Binzhou, Shandong 256600, PR China. FAU - Lv, Guangyao AU - Lv G AD - Department of Pharmacy, Binzhou Medical University Hospital, Binzhou, Shandong 256603, PR China. Electronic address: byfylgy@bzmc.edu.cn. FAU - Guo, Hongli AU - Guo H AD - School of Health, Binzhou Polytechnic, Binzhou, Shandong 256600, PR China. FAU - Gao, Xinfu AU - Gao X AD - Department of Pharmacy, Binzhou Medical University Hospital, Binzhou, Shandong 256603, PR China. Electronic address: byfygxf@163.com. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - Netherlands TA - Int J Biochem Cell Biol JT - The international journal of biochemistry & cell biology JID - 9508482 SB - IM OTO - NOTNLM OT - SLFN5 OT - biomarker OT - context dependence OT - metastasis OT - tumor suppressor COIS- Declaration of Competing Interest The authors declare no conflict of interest financial or otherwise. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:31 PHST- 2026/05/15 00:00 [received] PHST- 2026/10/27 00:00 [revised] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:31 [entrez] AID - S1357-2725(26)00168-8 [pii] AID - 10.1016/j.biocel.2026.107058 [doi] PST - aheadofprint SO - Int J Biochem Cell Biol. 2026 Oct 29:107058. doi: 10.1016/j.biocel.2026.107058. PMID- 42956962 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-4626 (Electronic) IS - 1091-255X (Linking) DP - 2026 Oct 29 TI - Letter to Editor regarding: Reconstruction and Patient Selection in Proximal Gastric Cancer: A Response to Copeland and Gajdos. PG - 102659 LID - S1091-255X(26)00340-9 [pii] LID - 10.1016/j.gassur.2026.102659 [doi] FAU - Polkowski, Wojciech P AU - Polkowski WP AD - Chair of Oncology, Medical University of Lublin, Lublin, Poland. Electronic address: wojciech.polkowski@umlub.edu.pl. LA - eng PT - Letter DEP - 20261029 PL - Netherlands TA - J Gastrointest Surg JT - Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract JID - 9706084 SB - IM COIS- Declaration of Competing Interests The author declares no competing interests. The author serves as the unpaid Coordinator of the European Chapter of the International Gastric Cancer Association (IGCA), as disclosed in the ICMJE form. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:31 PHST- 2026/10/19 00:00 [received] PHST- 2026/10/24 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:31 [entrez] AID - S1091-255X(26)00340-9 [pii] AID - 10.1016/j.gassur.2026.102659 [doi] PST - aheadofprint SO - J Gastrointest Surg. 2026 Oct 29:102659. doi: 10.1016/j.gassur.2026.102659. PMID- 42956959 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-4626 (Electronic) IS - 1091-255X (Linking) DP - 2026 Oct 29 TI - The Waitlist Paradox: Center Volume, Offer Acceptance, and Pseudo-Access to Liver Transplantation. PG - 102662 LID - S1091-255X(26)00343-4 [pii] LID - 10.1016/j.gassur.2026.102662 [doi] AB - BACKGROUND: Access to liver transplantation and post-transplant outcomes may vary across centers. We examined the association between center volume and waitlist outcomes among U.S. liver transplant programs. METHODS: Adult liver transplant programs were identified using Scientific Registry of Transplant Recipients program-specific reports and stratified into quartiles by deceased donor liver transplant (DDLT) volume. Metrics compared across quartiles included deceased-donor transplant rate ratio (DDTR ratio), time-to-transplant ratio, modeled offer acceptance ratio (OAR), waitlist outcomes, graft survival hazard ratios (HRs) and were reported as median and interquartile ranges (IQR). RESULTS: DDLT volume increased from Q1 to Q4 (10 [1-33] vs 315 [267-357]; p<0.001). Low-volume centers had lower DDTR ratio (0.35 [0.25-0.82] vs 1.36 [1.02-2.01]; p<0.001) and higher time-to-transplant ratios (1.69 [0.79-2.83] vs 0.82 [0.54-1.34]; p=0.043), while observed waitlist death rates were similar. Overall, OAR increased with volume (Q1 0.51 [0.31-1.05] vs Q4 1.20 [1.13-1.56]), including for PHS high-risk offers, hard-to-place offers, and donors >500 miles away (all p<0.001). Low-volume centers had lower deceased-donor waitlist transplant proportions (31.45% [20.00-41.23] vs 53.62% [46.46-59.86]) and overall waitlist transplant proportions (32.52% [20.50-44.35] vs 55.74% [49.40-61.54]), with higher removals for clinical deterioration, though similar waitlist death percentages (p=0.268). CONCLUSIONS: Low-volume centers demonstrated lower transplant rates, longer time to transplantation, and lower offer acceptance despite similar waitlist mortality, indicating lower realized access to transplantation among waitlisted candidates. These program-level associations warrant further investigation of candidate selection, offer availability, allocation pathways, and program resources before specific interventions are recommended. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Inc. All rights reserved. FAU - Alizai, Qaidar AU - Alizai Q AD - Department of Surgery, The Ohio State University Wexner Medical Center and James Comprehensive Cancer Center, Columbus, OH. FAU - Akabane, Miho AU - Akabane M AD - Department of Surgery, The Ohio State University Wexner Medical Center and James Comprehensive Cancer Center, Columbus, OH. FAU - Arena, Lorenza AU - Arena L AD - Department of Surgery, The Ohio State University Wexner Medical Center and James Comprehensive Cancer Center, Columbus, OH. FAU - Mevawalla, Areesh AU - Mevawalla A AD - Department of Surgery, The Ohio State University Wexner Medical Center and James Comprehensive Cancer Center, Columbus, OH. FAU - Angez, Meher AU - Angez M AD - Department of Surgery, The Ohio State University Wexner Medical Center and James Comprehensive Cancer Center, Columbus, OH. FAU - Ejaz, Rida AU - Ejaz R AD - Department of Surgery, The Ohio State University Wexner Medical Center and James Comprehensive Cancer Center, Columbus, OH. FAU - Elemosho, Abdulaziz AU - Elemosho A AD - Department of Surgery, The Ohio State University Wexner Medical Center and James Comprehensive Cancer Center, Columbus, OH. FAU - Endo, Yutaka AU - Endo Y AD - Division of Transplant Surgery, University of Rochester Medical Center, Rochester, NY. FAU - Schenk, Austin AU - Schenk A AD - Department of Surgery, The Ohio State University Wexner Medical Center and James Comprehensive Cancer Center, Columbus, OH. FAU - Pawlik, Timothy M AU - Pawlik TM AD - Department of Surgery, The Ohio State University Wexner Medical Center and James Comprehensive Cancer Center, Columbus, OH. Electronic address: tim.pawlik@osumc.edu. LA - eng PT - Journal Article DEP - 20261029 PL - Netherlands TA - J Gastrointest Surg JT - Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract JID - 9706084 SB - IM OTO - NOTNLM OT - Hospital Volume OT - Liver Transplantation OT - Organ Acceptance OT - Treatment Outcome OT - Waiting Lists EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:31 PHST- 2026/05/27 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:31 [entrez] AID - S1091-255X(26)00343-4 [pii] AID - 10.1016/j.gassur.2026.102662 [doi] PST - aheadofprint SO - J Gastrointest Surg. 2026 Oct 29:102662. doi: 10.1016/j.gassur.2026.102662. PMID- 42956935 OWN - NLM STAT- Publisher LR - 20261029 IS - 2468-7847 (Electronic) IS - 2468-7847 (Linking) DP - 2026 Oct 29 TI - CERVICAL CANCER IN OLDER WOMEN - A NARRATIVE REVIEW OF AGE-SPECIFIC MANAGEMENT ISSUES FROM THE FRENCH CERVICAL CANCER GUIDELINES GROUP. PG - 103317 LID - S2468-7847(26)00216-3 [pii] LID - 10.1016/j.jogoh.2026.103317 [doi] AB - OBJECTIVE: To summarize the available evidence on the management of cervical cancer in older women across early and advanced disease stages, with particular attention to treatment selection, feasibility, tolerance, and specific clinical situations, including cervical cancer associated with pelvic organ prolapse. METHODS: A narrative review was conducted using PubMed/MEDLINE, Embase, and the Cochrane Library. Studies addressing surgery, radiotherapy, concurrent chemoradiotherapy, brachytherapy, para-aortic lymphadenectomy, cervical cancer associated with pelvic organ prolapse, and pre-treatment geriatric assessment were considered. The evidence was synthesized qualitatively according to six predefined clinical questions. RESULTS: In early-stage disease, radical hysterectomy is feasible in selected fit elderly patients but is associated with increased perioperative morbidity, with advancing age. The available evidence does not establish whether surgery or definitive radiotherapy provides superior oncological outcomes in this population. In locally advanced cervical cancer, concurrent chemoradiotherapy appears to improve survival compared with radiotherapy alone in selected older patients, but with increased toxicity. Brachytherapy is associated with improved local control and survival, yet its use decreased with advancing age. No age-specific evidence demonstrated a survival benefit form pretreatment para-aortic lymphadenectomy. Data on cervical cancer associated with pelvic organ prolapse are limited to heterogeneous case reports and small retrospective studies. Pretreatment geriatric assessment influences treatment decisions in mixed cancer populations, but cervical cancer-specific outcome data are lacking. CONCLUSION: Age-specific evidence remains limited and largely retrospective, and chronological age alone should not determine access to standard treatment. Treatment selection requires integration of tumor characteristics, frailty, comorbidities, functional status, expected treatment tolerance, quality of life, and patient preferences. CI - Copyright (c) 2026. Published by Elsevier Masson SAS. FAU - Dugast, Margot AU - Dugast M AD - Universite de Rennes, service de gynecologie, CHU Rennes, Inserm, Irset (Institut de recherche en sante, environnement et travail) - UMRS 1085, F-35000 Rennes, France. FAU - Moureau, Anthony AU - Moureau A AD - Universite de Rennes, service de gynecologie, CHU Rennes, Inserm, Irset (Institut de recherche en sante, environnement et travail) - UMRS 1085, F-35000 Rennes, France. FAU - Tromelin, Estelle AU - Tromelin E AD - Universite de Rennes, service de gynecologie, CHU Rennes, Inserm, Irset (Institut de recherche en sante, environnement et travail) - UMRS 1085, F-35000 Rennes, France. FAU - Sousa, Carla AU - Sousa C AD - Universite de Rennes, service de gynecologie, CHU Rennes, Inserm, Irset (Institut de recherche en sante, environnement et travail) - UMRS 1085, F-35000 Rennes, France. FAU - Mercier, Marion AU - Mercier M AD - Universite de Rennes, service de gynecologie, CHU Rennes, Inserm, Irset (Institut de recherche en sante, environnement et travail) - UMRS 1085, F-35000 Rennes, France. FAU - Carcopino, Xavier AU - Carcopino X AD - Department of Obstetrics and Gynaecology, Hopital Nord, Assistance Publique-Hopitaux de Marseille (APHM), CEReSS, Center for Studies and Research on Health Services and Quality of Life, Aix Marseille University (AMU), Marseille, France. FAU - Lavoue, Vincent AU - Lavoue V AD - Universite de Rennes, service de gynecologie, CHU Rennes, Inserm, Irset (Institut de recherche en sante, environnement et travail) - UMRS 1085, F-35000 Rennes, France. Electronic address: Vincent.lavoue@chu-rennes.fr. FAU - Carcopino, Xavier AU - Carcopino X AD - Department of Obstetrics and Gynaecology, Hopital Nord, Assistance Publique-Hopitaux de Marseille (APHM), CEReSS, Center for Studies and Research on Health Services and Quality of Life, Aix Marseille University (AMU), Marseille, France. FAU - Ouldamer, Lobna AU - Ouldamer L AD - Department of Gynecology, Centre Hospitalier Universitaire de Tours, INSERM U1069; Universite Francois-Rabelais, Tours, France. FAU - Huchon, Cyrille AU - Huchon C AD - Department of Obstetrics and Gynaecology, Lariboisiere Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Paris Cite University, Paris, France. FAU - Asseeva, Pauline AU - Asseeva P AD - Department of Nuclear Medicine, Hopital Nord, Assistance Publique-Hopitaux de Marseille (APHM), Marseille, France. FAU - Azais, Henri AU - Azais H AD - Department of Gynecologic and Breast Cancer Surgery, Hopital Europeen Georges Pompidou, AP-HP, Paris Cite University, Paris, France. FAU - Bendifallah, Sofiane AU - Bendifallah S AD - Department of Gynaecologic Surgery, American Hospital of Paris, Neuilly-sur-Seine, France. FAU - Brun, Jean-Luc AU - Brun JL AD - Department of Gynaecologic Surgery and Reproductive Medicine, Alienor d'Aquitaine Center, Pellegrin Hospital, Bordeaux, France. FAU - Canlorbe, Geoffroy AU - Canlorbe G AD - Department of Gynaecologic and Breast Cancer Surgery, Pitie-Salpetriere Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Saint-Antoine Research Center (CRSA), INSERM UMR_S_938, Cancer Biology and Therapeutics, Paris, France. FAU - Chargari, Cyrus AU - Chargari C AD - Department of Radiation Oncology, Pitie-Salpetriere Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Paris, France. FAU - Chauvet, Pauline AU - Chauvet P AD - Department of Obstetrics and Gynaecology, Estaing University Hospital, Clermont Auvergne University, EnCoV, Institut Pascal, CNRS UMR 6602, SIGMA Clermont, Clermont-Ferrand, France. FAU - Cordoba, Abel AU - Cordoba A AD - Department of Radiation Therapy, Centre Oscar Lambret, Lille, France. FAU - Courcier, Helene AU - Courcier H AD - Department of Obstetrics and Gynaecology, Bichat Hospital, Paris Cite University, Paris, France. FAU - Dabi, Yohann AU - Dabi Y AD - Department of Obstetrics and Gynaecology and Reproductive Medicine, Tenon Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Paris, France. FAU - de Berti, Marion AU - de Berti M AD - Department of Gynecology, Centre Hospitalier Universitaire de Tours, INSERM U1069; Universite Francois-Rabelais, Tours, France. FAU - Diguisto, Caroline AU - Diguisto C AD - Department of Gynecology, Centre Hospitalier Universitaire de Tours, INSERM U1069; Universite Francois-Rabelais, Tours, France. FAU - Fuss, Eva AU - Fuss E AD - Department of Gynaecologic Surgery, Hautepierre University Hospital, Strasbourg, France. FAU - Gaillard, Thomas AU - Gaillard T AD - Department of Gynaecologic, Breast and Reconstructive Surgery, Institut Curie, Paris, France. FAU - Gauthier, Tristan AU - Gauthier T AD - Department of Obstetrics and Gynaecology, Women, Mothers and Children Hospital (HFME), Limoges University Hospital, Limoges, France. FAU - Gertych, Witold AU - Gertych W AD - Department of Gynaecologic and Oncologic Surgery, Lyon Sud Hospital, Lyon, France. FAU - Gladieff, Laurence AU - Gladieff L AD - Department of Medical Oncology, Oncopole Claudius Regaud, IUCT-Oncopole, Toulouse, France. FAU - Graesslin, Olivier AU - Graesslin O AD - Alix de Champagne Mother and Child Institute, Reims University Hospital, University of Reims Champagne-Ardenne (URCA), Reims, France. FAU - Kerbage, Yohan AU - Kerbage Y AD - Department of Gynaecologic Surgery, Lille University Hospital, University of Lille, INSERM U1189, OncoThAI: Laser-Assisted Therapies and Immunotherapies for Oncology, Lille, France. FAU - Koskas, Martin AU - Koskas M AD - Department of Obstetrics and Gynaecology, Bichat Hospital, Paris Cite University, Paris, France. FAU - Laas, Enora AU - Laas E AD - Department of Gynaecologic, Breast and Reconstructive Surgery, Institut Curie, Paris, France. FAU - Lacorre, Aymeline AU - Lacorre A AD - Department of Obstetrics and Gynaecology, Women, Mothers and Children Hospital (HFME), Limoges University Hospital, Limoges, France. FAU - Lavoue, Vincent AU - Lavoue V AD - Department of Gynaecology, Obstetrics and Human Reproduction, CHU Anne de Bretagne, Faculty of Medicine, University of Rennes, IRSET, INSERM U1085, Rennes, France. FAU - Lecointre, Lise AU - Lecointre L AD - Department of Gynaecologic Surgery, Hautepierre University Hospital, Strasbourg, France. FAU - Lecuru, Fabrice AU - Lecuru F AD - Department of Breast, Gynaecologic and Reconstructive Surgery, Institut Curie, Faculty of Medicine, Paris Cite University, Paris, France. FAU - Margueritte, Francois AU - Margueritte F AD - Department of Obstetrics and Gynaecology, Women, Mothers and Children Hospital (HFME), Limoges University Hospital, Limoges, France. FAU - Martinez, Alejandra AU - Martinez A AD - Oncopole Claudius Regaud, Toulouse University Cancer Institute-Oncopole, Toulouse III-Paul Sabatier University, INSERM, CNRS, University of Toulouse, Toulouse Cancer Research Center, UMR1037, Toulouse, France. FAU - Maternowski, Paul-Jean AU - Maternowski PJ AD - Department of Gynaecologic Surgery and Reproductive Medicine, Alienor d'Aquitaine Center, Pellegrin Hospital, Bordeaux, France. FAU - Mathevet, Patrice AU - Mathevet P AD - Lausanne University Hospital (CHUV), Lausanne, Switzerland; University of Lausanne (UNIL), Lausanne, Switzerland. FAU - Menouer, Imane AU - Menouer I AD - Alix de Champagne Mother and Child Institute, Reims University Hospital, University of Reims Champagne-Ardenne (URCA), Reims, France. FAU - Mimoun, Camille AU - Mimoun C AD - Department of Obstetrics and Gynaecology, Lariboisiere Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Paris Cite University, Paris, France. FAU - Moureau, Anthony AU - Moureau A AD - Department of Gynaecology, Obstetrics and Human Reproduction, CHU Anne de Bretagne, Faculty of Medicine, University of Rennes, IRSET, INSERM U1085, Rennes, France. FAU - Netter, Antoine AU - Netter A AD - Department of Obstetrics and Gynaecology, Hopital Nord, Assistance Publique-Hopitaux de Marseille (APHM), CEReSS, Center for Studies and Research on Health Services and Quality of Life, Aix Marseille University (AMU), Marseille, France. FAU - Philip, Charles-Andre AU - Philip CA AD - Women and Children Hospital, Hospices Civils de Lyon (HCL), LabTAU INSERM U1032, Claude Bernard Lyon 1 University, Lyon, France. FAU - Rousset, Pascal AU - Rousset P AD - Department of Medical and Interventional Imaging, Lyon Sud University Hospital, Hospices Civils de Lyon, Claude Bernard Lyon 1 University, EA CICLY, Lyon, France. FAU - Sanson, Claire AU - Sanson C AD - Department of Gynaecologic Surgery, Paris Saint-Joseph Hospital Group, Paris, France. FAU - Thomassin-Naggara, Isabelle AU - Thomassin-Naggara I AD - Department of Medical Imaging, Tenon Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Paris, France. FAU - Touboul, Cyril AU - Touboul C AD - Department of Obstetrics and Gynaecology and Reproductive Medicine, Tenon Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Paris, France. FAU - Leveque, Jean AU - Leveque J AD - Department of Gynaecology, Obstetrics and Human Reproduction, CHU Anne de Bretagne, Faculty of Medicine, University of Rennes, IRSET, INSERM U1085, Rennes, France. CN - members from the FRENCH CERVICAL CANCER GUIDELINES GROUP LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - France TA - J Gynecol Obstet Hum Reprod JT - Journal of gynecology obstetrics and human reproduction JID - 101701588 SB - IM OTO - NOTNLM OT - brachytherapy OT - cervical cancer OT - chemoradiotherapy OT - frailty OT - genital prolapse OT - geriatric assessment OT - older women OT - radiotherapy OT - surgery COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:30 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:30 [entrez] AID - S2468-7847(26)00216-3 [pii] AID - 10.1016/j.jogoh.2026.103317 [doi] PST - aheadofprint SO - J Gynecol Obstet Hum Reprod. 2026 Oct 29:103317. doi: 10.1016/j.jogoh.2026.103317. PMID- 42956934 OWN - NLM STAT- Publisher LR - 20261029 IS - 2468-7847 (Electronic) IS - 2468-7847 (Linking) DP - 2026 Oct 29 TI - Transvaginal Natural Orifice Transluminal Endoscopic Surgery (vNOTES) for adnexal torsion (detorsion followed by ovarian cystectomy) in 10 steps. PG - 103310 LID - S2468-7847(26)00209-6 [pii] LID - 10.1016/j.jogoh.2026.103310 [doi] AB - Adnexal torsion is a surgical emergency usually treated by laparoscopy. The transvaginal natural orifice transluminal endoscopic surgery (vNOTES) may be a relevant minimally invasive alternative to laparoscopy. We propose a step-by-step video of the adnexal detorsion followed by ovarian cystectomy by the vNOTES approach. CI - Copyright (c) 2026. Published by Elsevier Masson SAS. FAU - Chene, Gautier AU - Chene G AD - Department of Gynecology, Hopital Femme Mere Enfant, HFME, 59 boulevard Pinel, University hospital of Lyon, 69500 Bron, France; University Claude Bernard of Lyon 1, EMR 3738 CICLY, 69000 Lyon, France. Electronic address: chenegautier@yahoo.fr. FAU - Cerruto, Emanuele AU - Cerruto E AD - Department of Gynecology, Hopital Femme Mere Enfant, HFME, 59 boulevard Pinel, University hospital of Lyon, 69500 Bron, France. FAU - Nohuz, Erdogan AU - Nohuz E AD - Department of Gynecology, Hopital Femme Mere Enfant, HFME, 59 boulevard Pinel, University hospital of Lyon, 69500 Bron, France. LA - eng PT - Journal Article DEP - 20261029 PL - France TA - J Gynecol Obstet Hum Reprod JT - Journal of gynecology obstetrics and human reproduction JID - 101701588 SB - IM OTO - NOTNLM OT - adnexal torsion OT - ovarian cystectomy OT - transvaginal Natural Orifice transluminal Endoscopic Surgery OT - vNOTES COIS- Declaration of competing interest The authors have no conflicts of interest to disclose EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:30 PHST- 2026/01/10 00:00 [received] PHST- 2026/10/24 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:30 [entrez] AID - S2468-7847(26)00209-6 [pii] AID - 10.1016/j.jogoh.2026.103310 [doi] PST - aheadofprint SO - J Gynecol Obstet Hum Reprod. 2026 Oct 29:103310. doi: 10.1016/j.jogoh.2026.103310. PMID- 42956933 OWN - NLM STAT- Publisher LR - 20261029 IS - 2468-7847 (Electronic) IS - 2468-7847 (Linking) DP - 2026 Oct 29 TI - POST-TREATMENT SURVEILLANCE AND MANAGEMENT OF RECURRENT CERVICAL CANCER - A REVIEW FROM THE FRENCH CERVICAL CANCER GUIDELINES GROUP. PG - 103315 LID - S2468-7847(26)00214-X [pii] LID - 10.1016/j.jogoh.2026.103315 [doi] AB - OBJECTIVE: To review the available evidence on post-treatment follow-up and recurrence management after cervical cancer. METHODS: We conducted a narrative review of the literature on surveillance modalities, and the management of pelvic and nodal recurrences. RESULTS: Most recurrences occur within three years of treatment, but the survival benefit of detection before symptom onset remains unproven. After fertility-sparing surgery, HPV testing is sensitive for detecting asymptomatic pelvic recurrence. After hysterectomy, most recurrences are symptomatic, and clinical examination has a higher diagnostic yield than HPV testing or vaginal vault cytology. After concurrent chemoradiotherapy (CRT), persistent HPV infection is associated with an increased recurrence risk, but its limited specificity and the absence of a validated HPV-based surveillance strategy restrict its clinical utility. MRI and FDG PET/CT are useful for response assessment and investigation of suspected recurrence, but evidence supporting routine imaging of asymptomatic patients is lacking. Intensive follow-up schedules have not improved recurrence detection or survival. Evidence on recurrence management is predominantly retrospective. Pelvic recurrences after primary surgery have been treated with CRT, supplemented by brachytherapy for vaginal involvement. In selected patients with central pelvic recurrence after CRT, complete surgical resection may provide prolonged survival, but carries substantial morbidity. Nodal recurrences outside previously irradiated fields have been treated with CRT. CONCLUSION: Available evidence supports a predominantly clinical, treatment-adapted follow-up. The survival benefit of intensive surveillance, routine imaging, or detecting asymptomatic recurrence remains uncertain. Recurrence management requires individualized multidisciplinary assessment based on disease extent, disease-free interval, and previous treatments. CI - Copyright (c) 2026. Published by Elsevier Masson SAS. FAU - Buffeteau, Aurelie AU - Buffeteau A AD - Department of Gynecology, Pellegrin hospital, Bordeaux University Hospital, University of Bordeaux, CNRS UMR 5164 ImmunoConcept, Bordeaux, France. FAU - Chauvet, Pauline AU - Chauvet P AD - Department of Obstetrics and Gynaecology, Estaing University Hospital, Clermont Auvergne University, EnCoV, Institut Pascal, CNRS UMR 6602, SIGMA Clermont, Clermont-Ferrand, France. FAU - Cordoba, Abel AU - Cordoba A AD - Department of Radiation Oncology, centre de lutte contre le cancer Oscar-Lambret, Lille, France. FAU - Leveque, Jean AU - Leveque J AD - Department of Gynaecology, Obstetrics and Human Reproduction, CHU Anne de Bretagne, Faculty of Medicine, University of Rennes, IRSET, INSERM-U1085, Rennes, France. FAU - Rousset, Pascal AU - Rousset P AD - Department of Medical and Interventional Imaging, Lyon Sud University Hospital, Hospices Civils de Lyon, Claude Bernard Lyon 1 University, EA CICLY, Lyon, France. FAU - Thomassin, Isabelle AU - Thomassin I AD - Department of Medical Imaging, Tenon Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Paris, France. FAU - Carcopino, Xavier AU - Carcopino X AD - Department of Obstetrics and Gynaecology, Hopital Nord, Assistance Publique-Hopitaux de Marseille (APHM), CEReSS, Center for Studies and Research on Health Services and Quality of Life, Aix Marseille University (AMU), Marseille, France. FAU - Brun, Jean-Luc AU - Brun JL AD - Department of Gynecology, Pellegrin hospital, Bordeaux University Hospital, UMR 5234, University of Bordeaux, Bordeaux, France.. Electronic address: jean-luc.brun@chu-bordeaux.fr. CN - members from the FRENCH CERVICAL CANCER GUIDELINES GROUP LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - France TA - J Gynecol Obstet Hum Reprod JT - Journal of gynecology obstetrics and human reproduction JID - 101701588 SB - IM OTO - NOTNLM OT - HPV test OT - MRI OT - PET scanner OT - cervical cancer OT - follow-up OT - recurrence FIR - Carcopino, Xavier IR - Carcopino X IRAD- Department of Obstetrics & Gynaecology, Hopital Nord, Assistance Publique-Hopitaux de Marseille (APHM), CEReSS, Center for Studies and Research on Health Services and Quality of Life, Aix Marseille University (AMU), Marseille, France. FIR - Ouldamer, Lobna IR - Ouldamer L IRAD- Department of Gynecology, Centre Hospitalier Universitaire de Tours, INSERM U1069; Universite Francois-Rabelais, Tours, France. FIR - Huchon, Cyrille IR - Huchon C IRAD- Department of Obstetrics and Gynaecology, Lariboisiere Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Paris Cite University, Paris, France. FIR - Asseeva, Pauline IR - Asseeva P IRAD- Department of Nuclear Medicine, Hopital Nord, Assistance Publique-Hopitaux de Marseille (APHM), Marseille, France. FIR - Azais, Henri IR - Azais H IRAD- Department of Gynecologic and Breast Cancer Surgery, Hopital Europeen Georges Pompidou, AP-HP, Paris Cite University, Paris, France. FIR - Bendifallah, Sofiane IR - Bendifallah S IRAD- Department of Gynaecologic Surgery, American Hospital of Paris, Neuilly-sur-Seine, France. FIR - Brun, Jean-Luc IR - Brun JL IRAD- Department of Gynaecologic Surgery and Reproductive Medicine, Alienor d'Aquitaine Center, Hopital Pellegrin, Bordeaux University Hospital, Bordeaux, France. FIR - Canlorbe, Geoffroy IR - Canlorbe G IRAD- Department of Gynaecologic and Breast Cancer Surgery, Pitie-Salpetriere Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Saint-Antoine Research Center (CRSA), INSERM UMR_S_938, Cancer Biology and Therapeutics, Paris, France. FIR - Chargari, Cyrus IR - Chargari C IRAD- Department of Radiation Oncology, Pitie-Salpetriere Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Paris, France. FIR - Chauvet, Pauline IR - Chauvet P IRAD- Department of Obstetrics & Gynaecology, Estaing University Hospital, Clermont Auvergne University, EnCoV, Institut Pascal, CNRS UMR 6602, SIGMA Clermont, Clermont-Ferrand, France. FIR - Cordoba, Abel IR - Cordoba A IRAD- Department of Radiation Therapy, Centre Oscar Lambret, Lille, France. FIR - Courcier, Helene IR - Courcier H IRAD- Department of Obstetrics and Gynaecology, Bichat Hospital, Paris Cite University, Paris, France. FIR - Dabi, Yohann IR - Dabi Y IRAD- Department of Obstetrics and Gynaecology and Reproductive Medicine, Tenon Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Paris, France. FIR - de Berti, Marion IR - de Berti M IRAD- Department of Gynecology, Centre Hospitalier Universitaire de Tours, INSERM U1069; Universite Francois-Rabelais, Tours, France. FIR - Diguisto, Caroline IR - Diguisto C IRAD- Department of Gynecology, Centre Hospitalier Universitaire de Tours, INSERM U1069; Universite Francois-Rabelais, Tours, France. FIR - Fuss, Eva IR - Fuss E IRAD- Department of Gynaecologic Surgery, Hautepierre University Hospital, Strasbourg, France. FIR - Gaillard, Thomas IR - Gaillard T IRAD- Department of Gynaecologic, Breast and Reconstructive Surgery, Institut Curie, Paris, France. FIR - Gauthier, Tristan IR - Gauthier T IRAD- Department of Obstetrics and Gynaecology, Women, Mothers and Children Hospital (HFME), Limoges University Hospital, Limoges, France. FIR - Gertych, Witold IR - Gertych W IRAD- Department of Gynaecologic and Oncologic Surgery, Lyon Sud Hospital, Lyon, France. FIR - Gladieff, Laurence IR - Gladieff L IRAD- Department of Medical Oncology, Oncopole Claudius Regaud, IUCT-Oncopole, Toulouse, France. FIR - Graesslin, Olivier IR - Graesslin O IRAD- Alix de Champagne Mother and Child Institute, Reims University Hospital, University of Reims Champagne-Ardenne (URCA), Reims, France. FIR - Kerbage, Yohan IR - Kerbage Y IRAD- Department of Gynaecologic Surgery, Lille University Hospital, University of Lille, INSERM U1189, OncoThAI: Laser-Assisted Therapies and Immunotherapies for Oncology, Lille, France. FIR - Koskas, Martin IR - Koskas M IRAD- Department of Obstetrics and Gynaecology, Bichat Hospital, Paris Cite University, Paris, France. FIR - Laas, Enora IR - Laas E IRAD- Department of Gynaecologic, Breast and Reconstructive Surgery, Institut Curie, Paris, France. FIR - Lacorre, Aymeline IR - Lacorre A IRAD- Department of Obstetrics and Gynaecology, Women, Mothers and Children Hospital (HFME), Limoges University Hospital, Limoges, France. FIR - Lavoue, Vincent IR - Lavoue V IRAD- Department of Gynaecology, Obstetrics & Human Reproduction, CHU Anne de Bretagne, Faculty of Medicine, University of Rennes, IRSET, INSERM U1085, Rennes, France. FIR - Lecointre, Lise IR - Lecointre L IRAD- Department of Gynaecologic Surgery, Hautepierre University Hospital, Strasbourg, France. FIR - Lecuru, Fabrice IR - Lecuru F IRAD- Department of Breast, Gynaecologic and Reconstructive Surgery, Institut Curie, Faculty of Medicine, Paris Cite University, Paris, France. FIR - Margueritte, Francois IR - Margueritte F IRAD- Department of Obstetrics and Gynaecology, Women, Mothers and Children Hospital (HFME), Limoges University Hospital, Limoges, France. FIR - Martinez, Alejandra IR - Martinez A IRAD- Oncopole Claudius Regaud, Toulouse University Cancer Institute-Oncopole, Toulouse III-Paul Sabatier University, INSERM, CNRS, University of Toulouse, Toulouse Cancer Research Center, UMR1037, Toulouse, France. FIR - Maternowski, Paul-Jean IR - Maternowski PJ IRAD- Department of Gynaecologic Surgery and Reproductive Medicine, Alienor d'Aquitaine Center, Hopital Pellegrin, Bordeaux University Hospital, Bordeaux, France. FIR - Mathevet, Patrice IR - Mathevet P IRAD- Lausanne University Hospital (CHUV), Lausanne, Switzerland; University of Lausanne (UNIL), Lausanne, Switzerland. FIR - Menouer, Imane IR - Menouer I IRAD- Alix de Champagne Mother and Child Institute, Reims University Hospital, University of Reims Champagne-Ardenne (URCA), Reims, France. FIR - Mimoun, Camille IR - Mimoun C IRAD- Department of Obstetrics and Gynaecology, Lariboisiere Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Paris Cite University, Paris, France. FIR - Moureau, Anthony IR - Moureau A IRAD- Department of Gynaecology, Obstetrics & Human Reproduction, CHU Anne de Bretagne, Faculty of Medicine, University of Rennes, IRSET, INSERM U1085, Rennes, France. FIR - Netter, Antoine IR - Netter A IRAD- Department of Obstetrics & Gynaecology, Hopital Nord, Assistance Publique-Hopitaux de Marseille (APHM), CEReSS, Center for Studies and Research on Health Services and Quality of Life, Aix Marseille University (AMU), Marseille, France. FIR - Philip, Charles-Andre IR - Philip CA IRAD- Women and Children Hospital, Hospices Civils de Lyon (HCL), LabTAU INSERM U1032, Claude Bernard Lyon 1 University, Lyon, France. FIR - Rousset, Pascal IR - Rousset P IRAD- Department of Medical and Interventional Imaging, Lyon Sud University Hospital, Hospices Civils de Lyon, Claude Bernard Lyon 1 University, EA CICLY, Lyon, France. FIR - Sanson, Claire IR - Sanson C IRAD- Department of Gynaecologic Surgery, Paris Saint-Joseph Hospital Group, Paris, France. FIR - Thomassin-Naggara, Isabelle IR - Thomassin-Naggara I IRAD- Department of Medical Imaging, Tenon Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Paris, France. FIR - Touboul, Cyril IR - Touboul C IRAD- Department of Obstetrics and Gynaecology and Reproductive Medicine, Tenon Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Paris, France. FIR - Leveque, Jean IR - Leveque J IRAD- Department of Gynaecology, Obstetrics & Human Reproduction, CHU Anne de Bretagne, Faculty of Medicine, University of Rennes, IRSET, INSERM U1085, Rennes, France. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:30 PHST- 2026/08/19 00:00 [received] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:30 [entrez] AID - S2468-7847(26)00214-X [pii] AID - 10.1016/j.jogoh.2026.103315 [doi] PST - aheadofprint SO - J Gynecol Obstet Hum Reprod. 2026 Oct 29:103315. doi: 10.1016/j.jogoh.2026.103315. PMID- 42956915 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-6513 (Electronic) IS - 0885-3924 (Linking) DP - 2026 Oct 29 TI - Who Is My Hospice Physician? A Descriptive Study of the US Hospice Medical Director Workforce. LID - S0885-3924(26)01060-2 [pii] LID - 10.1016/j.jpainsymman.2026.10.018 [doi] AB - INTRODUCTION: In 2024, the Centers for Medicare & Medicaid Services (CMS) began requiring hospices to report their hospice medical director (HMD), enabling the first national study of this workforce. OBJECTIVE: To 1) characterize the specialty and subspecialty certifications of HMDs; 2) quantify the number with HMD certification; 3) assess the extent to which HMDs serve multiple hospices; and 4) examine the transparency of HMDs on hospice websites. METHODS: We queried the 2025 CMS Hospice All Owners file for HMDs. Resulting names were searched in Doximity and other sites to identify training background. Resulting names were also linked to HMD certification data from the Hospice Medical Director Certification Board (HMDCB). Finally, a random sample of 295 hospices was selected for web-based analysis of HMD information. RESULTS: We identified 2,387 HMDs across 2,914 agencies. Fewer than half of U.S. hospices reported HMDs to CMS. HMDs were certified in 23 specialties, most commonly Internal Medicine (47.6%). HMDs were certified in 28 different subspecialties, including 17.8% (n=424) with certification in Hospice and Palliative Medicine (HPM); 12.6% (n=301) were HMDCB certified. Regarding multi-agency affiliations, 85.0% (n=2,030) served one hospice, 13.2% (n=314) served 2-4 hospices, and 1.8% (n=43) served >/= 5 hospices (maximum 29). In a 10% random sample (n=295), approximately 88% of hospices did not list their HMD online. CONCLUSION: HPM board certification and formal HMD certification were uncommon, raising questions about how physicians are prepared for the clinical and leadership responsibilities of hospice medical directorship. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Soltoff, Alexander AU - Soltoff A AD - Department of Health Policy and Management, Emory University Rollins School of Public Health, Atlanta, Georgia (Soltoff); Division of Palliative Medicine, Emory University School of Medicine, Atlanta, Georgia (Cross, Kavalieratos); Division of Health Policy and Economics, Weill Cornell Medical College, New York, New York (Braun); Chapters Health System, Temple Terrace, FL (Friedman). Electronic address: alex.soltoff@emory.edu. FAU - Cross, Sarah AU - Cross S AD - Department of Health Policy and Management, Emory University Rollins School of Public Health, Atlanta, Georgia (Soltoff); Division of Palliative Medicine, Emory University School of Medicine, Atlanta, Georgia (Cross, Kavalieratos); Division of Health Policy and Economics, Weill Cornell Medical College, New York, New York (Braun); Chapters Health System, Temple Terrace, FL (Friedman). FAU - Braun, Robert Tyler AU - Braun RT AD - Department of Health Policy and Management, Emory University Rollins School of Public Health, Atlanta, Georgia (Soltoff); Division of Palliative Medicine, Emory University School of Medicine, Atlanta, Georgia (Cross, Kavalieratos); Division of Health Policy and Economics, Weill Cornell Medical College, New York, New York (Braun); Chapters Health System, Temple Terrace, FL (Friedman). FAU - Friedman, Tara AU - Friedman T AD - Department of Health Policy and Management, Emory University Rollins School of Public Health, Atlanta, Georgia (Soltoff); Division of Palliative Medicine, Emory University School of Medicine, Atlanta, Georgia (Cross, Kavalieratos); Division of Health Policy and Economics, Weill Cornell Medical College, New York, New York (Braun); Chapters Health System, Temple Terrace, FL (Friedman). FAU - Kavalieratos, Dio AU - Kavalieratos D AD - Department of Health Policy and Management, Emory University Rollins School of Public Health, Atlanta, Georgia (Soltoff); Division of Palliative Medicine, Emory University School of Medicine, Atlanta, Georgia (Cross, Kavalieratos); Division of Health Policy and Economics, Weill Cornell Medical College, New York, New York (Braun); Chapters Health System, Temple Terrace, FL (Friedman). LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Pain Symptom Manage JT - Journal of pain and symptom management JID - 8605836 SB - IM EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:30 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:30 [entrez] AID - S0885-3924(26)01060-2 [pii] AID - 10.1016/j.jpainsymman.2026.10.018 [doi] PST - aheadofprint SO - J Pain Symptom Manage. 2026 Oct 29:S0885-3924(26)01060-2. doi: 10.1016/j.jpainsymman.2026.10.018. PMID- 42956912 OWN - NLM STAT- Publisher LR - 20261029 IS - 1872-7913 (Electronic) IS - 0924-8579 (Linking) DP - 2026 Oct 29 TI - Accelerated Laboratory Evolution Generates Growth-Costly High-Transfer Variants of an mcr-1-Bearing IncI2 Resistance Plasmid. PG - 108047 LID - S0924-8579(26)00334-1 [pii] LID - 10.1016/j.ijantimicag.2026.108047 [doi] AB - OBJECTIVES: This study aimed to characterize the plasmid-associated changes and host fitness consequences accompanying experimentally enriched high-transfer variants of the mcr-1-bearing IncI2 plasmid p14E1050. METHODS: We established an Accelerated Laboratory Evolution (ALE) system that coupled pMP6-mediated mutagenesis with iterative selection for enhanced conjugation frequency of the mcr-1-bearing p14E1050. Evolved lineages were characterized using conjugation assays, plasmid copy number (PCN) quantification, growth curve measurement, RNA-seq, and whole-genome sequencing (WGS). RESULTS: Under selection for plasmid transmissibility, the mcr-1-bearing IncI2 plasmid p14E1050 rapidly evolved an enhanced conjugation phenotype, exhibiting a 41.05 +/- 14.77-fold increase in conjugation frequency. WGS identified 32 mutations within the plasmid backbone of evolved variants. Increased PCN was associated with elevated conjugation frequency, while transcriptomic analysis of independent high-transfer variants revealed shared host-plasmid transcriptional remodeling, including upregulation of conjugation-related genes and pathways involved in nucleotide metabolism. Notably, enhanced transmissibility was accompanied by reduced host growth performance, indicating a cost to host-plasmid compatibility. CONCLUSIONS: Accelerated laboratory evolution rapidly enriched high-transfer variants of the mcr-1-bearing IncI2 plasmid p14E1050, accompanied by increased PCN and reduced host growth. These findings suggest that high-transfer states can emerge during accelerated plasmid evolution, highlighting the relevance of evolutionary accessibility when evaluating antimicrobial resistance (AMR) plasmid dissemination risk. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Zhou, Shiying AU - Zhou S AD - State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China. FAU - Li, Jiahui AU - Li J AD - State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China. FAU - Deng, Mingyang AU - Deng M AD - State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China. FAU - Ren, Shiying AU - Ren S AD - State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China. FAU - Wang, Junhao AU - Wang J AD - State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China. FAU - Yingwen, Ouyang AU - Yingwen O AD - State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China. FAU - Long, Tengfei AU - Long T AD - Department of Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, Buffalo, NY 14214, United States. FAU - Xia, Lijuan AU - Xia L AD - State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China. FAU - Song, Yuhan AU - Song Y AD - State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China. FAU - Lian, Xinlei AU - Lian X AD - State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China. FAU - Zhao, Donghao AU - Zhao D AD - State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China. FAU - Liao, Xiaoping AU - Liao X AD - State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China. FAU - Chen, Liang AU - Chen L AD - Department of Pharmacy Practice, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, Buffalo, NY 14214, United States. FAU - Ren, Hao AU - Ren H AD - State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China. FAU - Li, Gong AU - Li G AD - State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China.; Institute of Animal Science, South China Agricultural University, Guangzhou, China. Electronic address: ligongdk@scau.edu.cn. FAU - Sun, Jian AU - Sun J AD - State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.; Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China.. Electronic address: jiansun@scau.edu.cn. LA - eng PT - Journal Article DEP - 20261029 PL - Netherlands TA - Int J Antimicrob Agents JT - International journal of antimicrobial agents JID - 9111860 SB - IM OTO - NOTNLM OT - Accelerated laboratory evolution OT - Antimicrobial resistance dissemination OT - Conjugative transfer OT - IncI2 plasmid OT - mcr-1 COIS- Declaration of competing interest All authors declare no conflicts of interest. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:30 PHST- 2026/05/29 00:00 [received] PHST- 2026/08/13 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:30 [entrez] AID - S0924-8579(26)00334-1 [pii] AID - 10.1016/j.ijantimicag.2026.108047 [doi] PST - aheadofprint SO - Int J Antimicrob Agents. 2026 Oct 29:108047. doi: 10.1016/j.ijantimicag.2026.108047. PMID- 42956908 OWN - NLM STAT- Publisher LR - 20261029 IS - 1872-7913 (Electronic) IS - 0924-8579 (Linking) DP - 2026 Oct 29 TI - Susceptibility, methodological discrepancies, heteroresistance, and iron acquisition-mediated cefiderocol resistance in Italian Pseudomonas aeruginosa: results from a multicenter prospective study. PG - 108044 LID - S0924-8579(26)00331-6 [pii] LID - 10.1016/j.ijantimicag.2026.108044 [doi] AB - Cefiderocol is a siderophore cephalosporin that leverages bacterial iron uptake systems to overcome resistance in Gram-negative pathogens. Despite strong in vitro activity against Pseudomonas aeruginosa, uncertainty remains regarding susceptibility testing accuracy and interpretation of borderline phenotypes. We conducted a prospective multicenter study of 352 non-duplicate clinical P. aeruginosa isolates from eight tertiary-care hospitals in Italy. The study assessed carbapenem resistance epidemiology, cefiderocol activity using reference and commercial methods, and methodological variability, including EUCAST-defined areas of technical uncertainty (ATU). Selected isolates underwent population analysis profiling, including strains showing growth within inhibition zones, and whole-genome sequencing of susceptible, heteroresistant, and resistant isolates. Carbapenem resistance was detected in 17.5% of isolates, with significant inter-hospital variation in carbapenemase distribution. Cefiderocol resistance was rare (0.3%) by reference testing, confirming sustained in vitro activity. Commercial susceptibility methods showed some variability in MIC values, particularly near clinical breakpoints. Disk diffusion occasionally identified isolates within the ATU, sometimes associated with discordant MIC results, although these discrepancies did not affect susceptibility categorization. Heteroresistance was uncommon (0.3%) and observed in a single isolate. Genomic analysis revealed alterations in iron acquisition pathways, including loss of pyoverdine biosynthesis genes in the resistant isolate, but no single genetic determinant fully explained reduced susceptibility, supporting a multifactorial resistance mechanism. Overall, cefiderocol retains strong in vitro activity against Italian P. aeruginosa isolates. However, variability in testing methods and the presence of intermediate phenotypes complicate interpretation. These findings underscore the need to refine susceptibility testing and better link MIC values, resistance mechanisms, and clinical outcomes. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved. FAU - Stracquadanio, Stefano AU - Stracquadanio S AD - Department of Medicine and Surgery, "Kore" University of Enna, Enna Italy. Electronic address: stefano.stracquadanio@unikore.it. FAU - Nicolosi, Alice AU - Nicolosi A AD - Department of Biomedical and Biotechnological Sciences - Section of Microbiology, University of Catania, Catania Italy. FAU - Privitera, Grete Francesca AU - Privitera GF AD - Department of Clinical and Experimental Medicine, Bioinformatic Unit, University of Catania, Via Santa Sofia, 95125 Catania, Italy. FAU - Bongiorno, Dafne AU - Bongiorno D AD - Department of Biomedical and Biotechnological Sciences - Section of Microbiology, University of Catania, Catania Italy. FAU - Calvo, Maddalena AU - Calvo M AD - U.O.C. Laboratory Analysis Unit, A.O.U. Policlinico "G. Rodolico-San Marco", Catania Italy. FAU - Migliorisi, Giuseppe AU - Migliorisi G AD - U.O.C. Laboratory Analysis Unit, A.O. "G.F. Ingrassia", Palermo Italy. FAU - Barone, Teresa AU - Barone T AD - U.O.C. Laboratory Analysis Unit, A.O. "G.F. Ingrassia", Palermo Italy. FAU - Perez, Marianna AU - Perez M AD - Azienda Ospedaliera per L'Emergenza, Cannizzaro, Catania Italy. FAU - Marra, Giovanna AU - Marra G AD - Azienda Ospedaliera per L'Emergenza, Cannizzaro, Catania Italy. FAU - Fasciana, Teresa AU - Fasciana T AD - Microbiology and Virology Unit, AOU Policlinico "P. Giaccone", Palermo Italy. FAU - Mannino, Erika AU - Mannino E AD - Microbiology and Virology Unit, AOU Policlinico "P. Giaccone", Palermo Italy. FAU - Giammanco, Giovanni AU - Giammanco G AD - Microbiology and Virology Unit, AOU Policlinico "P. Giaccone", Palermo Italy. FAU - Principe, Luigi AU - Principe L AD - Microbiology and Virology Unit, Great Metropolitan Hospital "Bianchi-Melacrino-Morelli", Reggio Calabria Italy. FAU - Marcuccio, Daniela AU - Marcuccio D AD - Microbiology and Virology Unit, Great Metropolitan Hospital "Bianchi-Melacrino-Morelli", Reggio Calabria Italy. FAU - Greco, Francesca AU - Greco F AD - Infectious and Tropical Diseases Unit, "Annunziata" Hub Hospital, Cosenza Italy. FAU - Mastroianni, Antonio AU - Mastroianni A AD - Infectious and Tropical Diseases Unit, "Annunziata" Hub Hospital, Cosenza Italy. FAU - Trecarichi, Enrico Maria AU - Trecarichi EM AD - Department of Life Science, Health, and Health Professions, Link Campus University, Rome Italy. FAU - Quirino, Angela AU - Quirino A AD - Unit of Clinical Microbiology, Department of Health Sciences, "Magna Graecia" University of Catanzaro, Catanzaro Italy. FAU - Matera, Giovanni AU - Matera G AD - Unit of Clinical Microbiology, Department of Health Sciences, "Magna Graecia" University of Catanzaro, Catanzaro Italy. FAU - Cricca, Monica AU - Cricca M AD - Unit of Microbiology, The Greater Romagna Area Hub Laboratory, Cesena Italy. FAU - Sambri, Vittorio AU - Sambri V AD - Unit of Microbiology, The Greater Romagna Area Hub Laboratory, Cesena Italy. FAU - Stefani, Stefania AU - Stefani S AD - Department of Biomedical and Biotechnological Sciences - Section of Microbiology, University of Catania, Catania Italy. LA - eng PT - Journal Article DEP - 20261029 PL - Netherlands TA - Int J Antimicrob Agents JT - International journal of antimicrobial agents JID - 9111860 SB - IM OTO - NOTNLM OT - Antimicrobial Susceptibility Testing OT - Carbapenems OT - Cefiderocol OT - Heteroresistance OT - P. aeruginosa COIS- Declaration of competing interest None EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:30 PHST- 2026/05/19 00:00 [received] PHST- 2026/09/07 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:30 [entrez] AID - S0924-8579(26)00331-6 [pii] AID - 10.1016/j.ijantimicag.2026.108044 [doi] PST - aheadofprint SO - Int J Antimicrob Agents. 2026 Oct 29:108044. doi: 10.1016/j.ijantimicag.2026.108044. PMID- 42956904 OWN - NLM STAT- Publisher LR - 20261029 IS - 1464-3405 (Electronic) IS - 0960-894X (Linking) DP - 2026 Oct 29 TI - 5,2''-di-O-alkylated gentamicin C1a analogs potentiate rifampicin against multidrug-resistant gram-negative bacteria. PG - 130811 LID - S0960-894X(26)00278-7 [pii] LID - 10.1016/j.bmcl.2026.130811 [doi] AB - O-alkylated gentamicin C1a analogs were prepared in 3-5 steps from gentamicin C1a using phase transfer-catalyzed O-alkylation. The O-alkylated gentamicin C1a analogs lost their standalone antibacterial activity but instead synergized the outer membrane-impermeable antibiotic rifampicin. 5,2''-di-O-alkylated-gentamicin C1a analogs 4 and 6 bearing biphenyl- or octyl- but not phenyl-substituents synergized rifampicin against a panel of Gram-negative bacteria. At concentrations of 8 mug/mL of 4 or 6 (~ 6 mmol), Gram-positive-selective antibiotic rifampicin was 32- to 512-fold potentiated against Gram-negative pathogens including multidrug-resistant Acinetobacter baumannii, Escherichia coli, and Pseudomonas aeruginosa isolates. Time-kill studies elucidated that the dual combination of 4 and rifampicin was bacteriostatic in A. baumannii AB031. The proposed mechanism of outer membrane permeabilization was verified by the reduced rifampicin potentiation in the presence of Mg(2+), as well as the absence of activity and synergy with rifampicin in Gram-positive bacteria. These findings indicate that di-O-alkylated gentamicin C1a analogs 4 and 6 are two new members of aminoglycoside-based outer membrane permeabilizers capable of potentiating rifampicin against Gram-negative pathogens. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Ramirez, Danzel M AU - Ramirez DM AD - Department of Chemistry, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. FAU - Mahesha, Chikkagundagal K AU - Mahesha CK AD - Department of Chemistry, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. FAU - Ramirez, Danyel AU - Ramirez D AD - Department of Chemistry, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. FAU - Dhiman, Shiv AU - Dhiman S AD - Department of Chemistry, University of Manitoba, Winnipeg, MB R3T 2N2, Canada. FAU - Schweizer, Frank AU - Schweizer F AD - Department of Chemistry, University of Manitoba, Winnipeg, MB R3T 2N2, Canada; Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB R3E 0J9, Canada. Electronic address: Frank.Schweizer@umanitoba.ca. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Bioorg Med Chem Lett JT - Bioorganic & medicinal chemistry letters JID - 9107377 SB - IM OTO - NOTNLM OT - Antibiotic Potentiator OT - Antibiotic adjuvant OT - Gentamicin OT - Gram-negative Bacteria OT - Outer membrane Permeabilizer OT - Rifampicin OT - Synergy COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:29 PHST- 2026/09/22 00:00 [received] PHST- 2026/10/24 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:29 [entrez] AID - S0960-894X(26)00278-7 [pii] AID - 10.1016/j.bmcl.2026.130811 [doi] PST - aheadofprint SO - Bioorg Med Chem Lett. 2026 Oct 29:130811. doi: 10.1016/j.bmcl.2026.130811. PMID- 42956903 OWN - NLM STAT- Publisher LR - 20261029 IS - 1527-9995 (Electronic) IS - 0090-4295 (Linking) DP - 2026 Oct 29 TI - Patient Characteristics and Outcomes of Urethral Ligation: A TURNS Study. LID - S0090-4295(26)00684-9 [pii] LID - 10.1016/j.urology.2026.10.027 [doi] AB - OBJECTIVE: To characterize patient selection and midterm outcomes after urethral ligation (UL) for end-stage outlet pathology across multiple reconstructive centers. METHODS: We retrospectively identified men undergoing UL at seven Trauma and Urologic Reconstructive Network of Surgeons centers from May 2005 through June 2025. The primary outcome was recurrent incontinence. Secondary outcomes included revision, 30-day readmission, urinary tract infection (UTI), urethral fistula, and abscess. Cumulative risks of recurrence and revision were estimated using Kaplan-Meier methods. RESULTS: Twenty-seven men underwent UL at a median age of 72 years (IQR 48-81). Common indications included urethral stricture/devastated urethra (29.6%), refractory incontinence (29.6%), failed bladder neck closure (25.9%), and artificial urinary sphincter erosion (22.2%). Urinary drainage was managed with a suprapubic tube in 19 patients, a continent catheterizable channel in 7, and ileovesicostomy in 1. Median follow-up was 24 months (IQR, 4-48); 11 patients (40.7%) had less than 1 year of follow-up. Three recurrences occurred within 3 months. Recurrence probability was 11.6% (95% CI, 3.9%-32.9%) and remained unchanged through 4 years. Revision risk was 3.7% (95% CI, 0.5%-23.5%). Thirty-day readmission occurred in 5 patients (18.5%). Among 16 patients with at least 1 year of follow-up, 11 (68.8%) developed UTI, and 3 (18.8%) developed urethral fistula. CONCLUSIONS: In a multi-institutional cohort, urethral ligation achieved high midterm success with low risk of revision surgery or fistulation. The burden of infection and readmission highlights the need for structured postoperative surveillance and bladder-directed interventions. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Abbasi, Behzad AU - Abbasi B AD - Department of Urology, University of California, San Francisco, San Francisco, CA, USA. FAU - Faris, Anna AU - Faris A AD - University of Michigan, West Shore Urology, Muskegon, MI, USA. FAU - Rourke, Keith F AU - Rourke KF AD - Division of Urology, University of Alberta, Edmonton, Alberta, Canada. FAU - Zhao, Lee C AU - Zhao LC AD - Department of Urology, New York University Langone Medical Center, New York City, NY, USA. FAU - Cohen, Andrew J AU - Cohen AJ AD - James Buchanan Brady Urological Institute, Johns Hopkins Medical Institutions, Baltimore, MD, USA. FAU - Wallace, Brendan K AU - Wallace BK AD - James Buchanan Brady Urological Institute, Johns Hopkins Medical Institutions, Baltimore, MD, USA. FAU - Smith, Thomas G 3rd AU - Smith TG 3rd AD - Department of Urology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. FAU - Elliott, Sean P AU - Elliott SP AD - Department of Urology, University of Minnesota, Minneapolis, MN, USA. FAU - Broghammer, Joshua A AU - Broghammer JA AD - Department of Urology, University of Kansas, Kansas City, KS, USA. FAU - Erickson, Bradley A AU - Erickson BA AD - Department of Urology, University of Iowa, Iowa City, IA, USA. FAU - Myrga, John M AU - Myrga JM AD - Department of Urology, University of California, San Francisco, San Francisco, CA, USA. FAU - Hampson, Lindsay A AU - Hampson LA AD - Department of Urology, University of California, San Francisco, San Francisco, CA, USA; San Francisco Veterans Affairs Medical Center. FAU - Breyer, Benjamin N AU - Breyer BN AD - Department of Urology, University of California, San Francisco, San Francisco, CA, USA; Department of Epidemiology and Biostatistics, University of California, San Francisco, San Francisco, CA, USA. Electronic address: Benjamin.Breyer@ucsf.edu. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Urology JT - Urology JID - 0366151 SB - IM COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:29 PHST- 2026/07/22 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:29 [entrez] AID - S0090-4295(26)00684-9 [pii] AID - 10.1016/j.urology.2026.10.027 [doi] PST - aheadofprint SO - Urology. 2026 Oct 29:S0090-4295(26)00684-9. doi: 10.1016/j.urology.2026.10.027. PMID- 42956901 OWN - NLM STAT- Publisher LR - 20261029 IS - 2468-1210 (Electronic) IS - 2468-1210 (Linking) DP - 2026 Oct 29 TI - Penetrating Injury to a Superficially Displaced Deep Ulnar Motor Branch in Piso-Hamate Coalition: Direct Repair Facilitated by Selective Osseous Grooving - A Case Report. PG - 103047 LID - S2468-1229(26)00484-6 [pii] LID - 10.1016/j.hansur.2026.103047 [doi] AB - Piso-hamate coalition may displace the deep motor branch of the ulnar nerve superficially over the osseous bridge. We report a 25-year-old man with a retained glass fragment and a posttraumatic neuroma one year after a penetrating hand injury. At surgery, the branch lay directly within the trajectory of the foreign body. After neuroma excision, the resulting nerve gap of 8 mm precluded tension-free repair. A longitudinal groove was created in the superficial aspect of the osseous bridge, shortening and protecting the nerve course and enabling direct tension-free neurorrhaphy. Qualitative dynamic radiographs showed hamato-triquetral widening in radial deviation, which contributed to the decision to preserve most of the coalition. Piso-hamate coalition may predispose the deep motor branch to penetrating injury. This case demonstrates the technical feasibility of selective osseous grooving to facilitate direct repair of the deep motor branch. CI - Copyright (c) 2026. Published by Elsevier Masson SAS. FAU - Kunz, Alexandra AU - Kunz A AD - Department of Plastic, Reconstructive and Aesthetic Surgery and Hand Surgery, Lausanne University Hospital (CHUV), Lausanne, Switzerland. FAU - Harder, Yves AU - Harder Y AD - Department of Plastic, Reconstructive and Aesthetic Surgery and Hand Surgery, Lausanne University Hospital (CHUV), Lausanne, Switzerland; Faculty of Biology and Medicine, University of Lausanne (UNIL), Lausanne, Switzerland. FAU - Durand, Sebastien AU - Durand S AD - Department of Plastic, Reconstructive and Aesthetic Surgery and Hand Surgery, Lausanne University Hospital (CHUV), Lausanne, Switzerland; Faculty of Biology and Medicine, University of Lausanne (UNIL), Lausanne, Switzerland. Electronic address: sebastien.durand@chuv.ch. LA - eng PT - Case Reports PT - Journal Article DEP - 20261029 PL - France TA - Hand Surg Rehabil JT - Hand surgery & rehabilitation JID - 101681801 SB - IM OTO - NOTNLM OT - Deep motor branch of the ulnar nerve OT - Direct nerve repair OT - Neuroma OT - Partial coalition resection OT - Penetrating hand injury OT - Piso-hamate coalition EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:29 PHST- 2026/09/23 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:29 [entrez] AID - S2468-1229(26)00484-6 [pii] AID - 10.1016/j.hansur.2026.103047 [doi] PST - aheadofprint SO - Hand Surg Rehabil. 2026 Oct 29:103047. doi: 10.1016/j.hansur.2026.103047. PMID- 42956892 OWN - NLM STAT- Publisher LR - 20261029 IS - 2352-345X (Electronic) IS - 2352-345X (Linking) DP - 2026 Oct 29 TI - Persistent Hepatocyte Plasticity and Lipid Metabolic Reprogramming After Hepatitis C Virus Cure. PG - 101927 LID - S2352-345X(26)00205-5 [pii] LID - 10.1016/j.jcmgh.2026.101927 [doi] AB - BACKGROUND AND AIMS: Chronic hepatitis C virus (HCV) infection disrupts hepatocyte polarity and promotes epithelial-mesenchymal transition (EMT), contributing to liver disease progression. Although direct-acting antivirals (DAAs) eradicate HCV infection in most patients, the risk of fibrosis progression and hepatocellular carcinoma persists after viral clearance. Whether antiviral cure fully restores hepatocyte differentiation, polarity, and epithelial identity programs remains unclear. We investigated whether HCV-induced EMT and lipid metabolism alterations are fully reversible following DAA treatment. METHODS: Huh7 hepatoma cells and Huh7 cells stably expressing a full-length HCV replicon were cultured in three-dimensional Matrigel to assess morphogenesis, polarity-associated features, and EMT. Spheroid morphology, canalicular-like organization, EMT markers, and transcriptomic signatures were analyzed using microscopy, immunofluorescence, RT-qPCR, and microarray analysis. The effects of DAA treatment (sofosbuvir with or without velpatasvir) were evaluated in vitro. Publicly available transcriptomic data from liver biopsies collected before therapy and within five days after its completion, were analyzed. RESULTS: HCV replicon expression altered canalicular-like organization and induced actin-rich protrusions in 90% of Huh7-R spheroids. DAA treatment efficiently suppressed HCV replicon expression and partially restored epithelial morphology, reducing protrusions to 25%, without normalizing them to the control level (2.5%). EMT-associated changes, including reduced E-cadherin and increased N-cadherin expression, were only partially corrected by DAA treatment. Transcriptomic EMT scoring showed persistent mesenchymal features in DAA-treated cells and in patient biopsies collected at the end of treatment. In parallel, genes involved in lipid metabolism and hepatocyte differentiation remained dysregulated. CONCLUSIONS: These findings show incomplete restoration of epithelial organization after suppression of HCV replicon expression in vitro and incomplete normalization of selected transcriptional signatures at the end of DAA treatment in patient liver samples. They suggest that cellular plasticity and metabolic reprogramming may persist after treatment and contribute to long-term liver disease risk. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Inc. All rights reserved. FAU - Agnetti, Jean AU - Agnetti J AD - INSERM, Unite 1193, Villejuif, F-94800, France; Universite Paris-Saclay, UMR-S 1193, Villejuif, F-94800, France. FAU - Cherief, Samy AU - Cherief S AD - INSERM, Unite 1193, Villejuif, F-94800, France; Universite Paris-Saclay, UMR-S 1193, Villejuif, F-94800, France. FAU - Desterke, Christophe AU - Desterke C AD - Universite Paris- Saclay, UFR Medecine-INSERMS1310, Villejuif, France. FAU - Bou-Malham, Vanessa AU - Bou-Malham V AD - INSERM, Unite 1193, Villejuif, F-94800, France; Universite Paris-Saclay, UMR-S 1193, Villejuif, F-94800, France. FAU - Benzoubir, Nassima AU - Benzoubir N AD - INSERM, Unite 1193, Villejuif, F-94800, France; Universite Paris-Saclay, UMR-S 1193, Villejuif, F-94800, France. FAU - Tan, Tuan Zea AU - Tan TZ AD - Cancer Science Institute of Singapore National University of Singapore, Center for Translational Medicine, 14 Medical Drive, 117599 Singapore, Singapore. FAU - Thiery, Jean Paul AU - Thiery JP AD - Guangzhou Laboratory, International biological Island Guangzhou, 510005 Guangzhou, China. FAU - Samuel, Didier AU - Samuel D AD - INSERM, Unite 1193, Villejuif, F-94800, France; Universite Paris-Saclay, UMR-S 1193, Villejuif, F-94800, France; AP-HP Hopital Paul Brousse, Centre Hepato-Biliaire, F-94800 Villejuif, France. FAU - Gassama-Diagne, Ama AU - Gassama-Diagne A AD - INSERM, Unite 1193, Villejuif, F-94800, France; Universite Paris-Saclay, UMR-S 1193, Villejuif, F-94800, France. Electronic address: ama.gassama@inserm.fr. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Cell Mol Gastroenterol Hepatol JT - Cellular and molecular gastroenterology and hepatology JID - 101648302 SB - IM OTO - NOTNLM OT - EMT OT - Hepatitis C virus OT - direct-acting antivirals OT - epithelial plasticity OT - hepatocyte polarity OT - lipid metabolism EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:29 PHST- 2026/01/10 00:00 [received] PHST- 2026/10/20 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:29 [entrez] AID - S2352-345X(26)00205-5 [pii] AID - 10.1016/j.jcmgh.2026.101927 [doi] PST - aheadofprint SO - Cell Mol Gastroenterol Hepatol. 2026 Oct 29:101927. doi: 10.1016/j.jcmgh.2026.101927. PMID- 42956882 OWN - NLM STAT- Publisher LR - 20261029 IS - 1872-7786 (Electronic) IS - 0009-2797 (Linking) DP - 2026 Oct 29 TI - Involvement of PINK1-related Mitophagy and Necroptosis in TDI-Induced Experimental Asthma. PG - 112406 LID - S0009-2797(26)00514-4 [pii] LID - 10.1016/j.cbi.2026.112406 [doi] AB - Toluene diisocyanate (TDI) is a prevalent cause of occupational asthma. Although mitophagy contributes to various pulmonary diseases, its role and downstream signaling in TDI-induced asthma remain unclear. In this study, we first utilized BALB/c mice to investigate the effects of TDI exposure on pulmonary injury, mitophagy, and associated regulatory mechanisms. Based on these findings, we constructed an adverse outcome pathway (AOP) network for TDI-induced asthma. Mechanistic connections predicted by the AOP were validated in vitro using PINK1-knockdown bronchial epithelial cells, assessing PINK1-related mitophagy, mitochondrial function, cytosolic mtDNA release, attenuated the increased expression of cGAS/STING pathway-related proteins, and necroptosis. Finally, we predicted potential natural product therapeutics through bioactivity screening and toxicity profiling, targeting key genes implicated in TDI-induced asthma. Integration of in vivo, in vitro, and AOP network analyses indicated that TDI exposure induced airway hyperresponsiveness and pulmonary histopathological changes, along with elevated Th2 cytokine production. This exposure selectively upregulated PINK1 and Parkin expression, without altering levels of BNIP3, NIX, or FUNDC1. PINK1 knockdown mitigated TDI-induced mitophagy-related alterations, restored mitochondrial function, reduced cytosolic mtDNA release, attenuated the increased expression of cGAS/STING pathway-related proteins, and reduced necroptosis. In silico screening predicted beta-lapachone, genistein, and apigenin as promising candidate therapeutics for TDI-induced asthma. Overall, PINK1-related mitophagy may contribute to TDI-induced occupational asthma, and natural compounds identified via in silico screening are potential candidates that require further in vitro and in vivo validation of their safety and efficacy. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - He, Xiu AU - He X AD - School of Public Health, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, China. Electronic address: hexiu@gmc.edu.cn. FAU - Fu, Shuai AU - Fu S AD - School of Public Health, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, China. FAU - Yao, Dengxiang AU - Yao D AD - School of Public Health, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, China. FAU - Zuo, Lijuan AU - Zuo L AD - School of Public Health, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, China. FAU - Yuan, Xiaoli AU - Yuan X AD - School of Public Health, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, China. FAU - Du, Yiyang AU - Du Y AD - School of Public Health, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, China. FAU - Qian, Zhiyao AU - Qian Z AD - School of Public Health, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, China. FAU - Ban, Jiaqi AU - Ban J AD - School of Public Health, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, China. FAU - He, Qian AU - He Q AD - Department of General Medicine, The Affiliated Hospital of Guizhou Medical University, Guiyang, 561113, China. Electronic address: 2023140020721@stu.gmc.edu.cn. FAU - You, Mingdan AU - You M AD - School of Public Health, Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang 561113, China. Electronic address: youmingdan@gmc.edu.cn. LA - eng PT - Journal Article DEP - 20261029 PL - Ireland TA - Chem Biol Interact JT - Chemico-biological interactions JID - 0227276 SB - IM OTO - NOTNLM OT - Adverse outcome pathway OT - Mitophagy OT - Necroptosis OT - Occupational asthma OT - PINK1 OT - Toluene diisocyanate COIS- Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:29 PHST- 2026/06/04 00:00 [received] PHST- 2026/09/06 00:00 [revised] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:29 [entrez] AID - S0009-2797(26)00514-4 [pii] AID - 10.1016/j.cbi.2026.112406 [doi] PST - aheadofprint SO - Chem Biol Interact. 2026 Oct 29:112406. doi: 10.1016/j.cbi.2026.112406. PMID- 42956879 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-2763 (Electronic) IS - 1873-2763 (Linking) DP - 2026 Oct 29 TI - Development and validation of a model using thoracic vertebrae data from low-dose CT scans for detecting AI-QCT-defined lumbar osteoporosis. PG - 118154 LID - S8756-3282(26)00380-7 [pii] LID - 10.1016/j.bone.2026.118154 [doi] AB - Despite evidence supporting opportunistic lumbar osteoporosis screening with low-dose computed tomography (LDCT), a clinically deployable screening strategy is lacking. This study aimed to develop and validate a model using thoracic bone mineral density (BMD) from LDCT for opportunistic screening. This multi-center, cross-sectional study included 18,298 adults who underwent LDCT with coverage from T1 to L2 at 134 Chinese health check-up centers. Outcome was defined as "artificial intelligence quantitative computed tomography (AI-QCT) defined lumbar osteoporosis" based on a mean L1-L2 BMD < 80 mg/cm(3) by phantom-less AI-QCT system. Five machine learning models were trained on T1-T12 BMD (n = 11,065) and evaluated internally (n = 4743) and externally (n = 2490). Performance metrics included accuracy, area under the receiver operating characteristic curve (AUC), sensitivity, specificity, positive predictive value, negative predictive value, Brier score, F1 score and area under the precision-recall curve (AUPRC). AI-QCT-defined lumbar osteoporosis was present in 1730 (9.5%) participants. Higher thoracic BMD was inversely associated with the AI-QCT-defined lumbar osteoporosis. T12 exhibited the strongest association: each 1 mg/cm(3) increment reduced odds by 21.9% (OR: 0.781, 95% confidence interval [CI]: 0.769, 0.793, P < 0.001). Logistic regression was selected as the final model. In external validation, it achieved an AUC of 0.993 (95% CI: 0.991, 0.996), AUPRC of 0.922 (95% CI: 0.887, 0.951), F1 score (0.813), sensitivity (0.975), and specificity (0.963). AI-QCT-derived thoracic BMD showed potential for identifying AI-QCT-defined lumbar osteoporosis. Integrating phantomless AI-QCT into lung cancer screening may enable large-scale opportunistic screening, but prospective validation against independent standards is required. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Zhang, Yichi AU - Zhang Y AD - Department of Endocrinology, Peking University First Hospital, Beijing, China; Clinical Research Institute, Institute of Advanced Clinical Medicine, Peking University, Beijing, China; Key Laboratory of Epidemiology of Major Diseases (Peking University), Ministry of Education, Beijing, China. FAU - Rong, Ce AU - Rong C AD - iKang Guobin Healthcare Group, Beijing, 100022, China. FAU - Zhang, Yanyu AU - Zhang Y AD - Department of Endocrinology, Peking University First Hospital, Beijing, China; Clinical Research Institute, Institute of Advanced Clinical Medicine, Peking University, Beijing, China; Key Laboratory of Epidemiology of Major Diseases (Peking University), Ministry of Education, Beijing, China. FAU - Wang, Zhaohui AU - Wang Z AD - iKang Guobin Healthcare Group, Beijing, 100022, China. FAU - Xiao, Hainan AU - Xiao H AD - iKang Guobin Healthcare Group, Beijing, 100022, China. FAU - Chen, Juan AU - Chen J AD - iKang Guobin Healthcare Group, Beijing, 100022, China. FAU - Liang, Yao AU - Liang Y AD - iKang Guobin Healthcare Group, Beijing, 100022, China. FAU - Yang, Xiaoxia AU - Yang X AD - iKang Guobin Healthcare Group, Beijing, 100022, China. FAU - Xie, Wuxiang AU - Xie W AD - Department of Endocrinology, Peking University First Hospital, Beijing, China; Clinical Research Institute, Institute of Advanced Clinical Medicine, Peking University, Beijing, China; Key Laboratory of Epidemiology of Major Diseases (Peking University), Ministry of Education, Beijing, China. Electronic address: xiewuxiang@hsc.pku.edu.cn. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Bone JT - Bone JID - 8504048 SB - IM OTO - NOTNLM OT - Bone mineral density OT - Low-dose computed tomography OT - Lumbar osteoporosis OT - Machine learning OT - Opportunistic screening COIS- Declaration of competing interest Ce Rong, Zhaohui Wang, Hainan Xiao, Juan Chen, Yao Liang, and Xiaoxia Yang are employees of iKang Guobin Healthcare Group, which is the data provider, the operator of the participating health examination centers, and the institution whose ethics committee approved this study. The AI-QCT system (HY-QCT-BMD, Huiying Medical Technology, software version V2.5.0, registration no. 20222210119) was procured by iKang Guobin Healthcare Group and used in its affiliated check-up centers; this constitutes an institutional procurement and clinical-use relationship, including only the standard end-user software license. iKang Guobin Healthcare Group has no employment, consulting, intellectual property, licensing, equity, or development relationship with Huiying Medical Technology beyond this procurement. No author has any personal commercial, employment, consulting, intellectual property, licensing, equity, or development relationship with Huiying Medical Technology, and no author has received personal fees, honoraria, or equity from Huiying Medical Technology. All other authors declare that they have no competing interests. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:29 PHST- 2026/08/21 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/24 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:29 [entrez] AID - S8756-3282(26)00380-7 [pii] AID - 10.1016/j.bone.2026.118154 [doi] PST - aheadofprint SO - Bone. 2026 Oct 29:118154. doi: 10.1016/j.bone.2026.118154. PMID- 42956876 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-0003 (Electronic) IS - 0141-8130 (Linking) DP - 2026 Oct 29 TI - Fluorescent zinc doped carbon quantum dots as active fillers in carboxymethyl cellulose/whey protein films for selective microbial sensing and enhanced apple packaging. PG - 155009 LID - S0141-8130(26)04961-5 [pii] LID - 10.1016/j.ijbiomac.2026.155009 [doi] AB - This study reports dual-functional active/intelligent composite films based on carboxymethyl cellulose (CMCe) and whey protein (WP) reinforced with fluorescent, zinc-doped carbon quantum dots (Zn-CQDs) derived from sustainable sugarcane bagasse. Microwave-assisted synthesis yielded ultra-small spherical CQDs (2.35-2.90 nm) with diffuse amorphous SAED rings, which shifted into template-coordinated crystalline Zn-CQDs (1.80-1.85 nm) with sharper diffraction profiles. Liquid-phase CFU assays demonstrated powerful concentration-dependent antimicrobial kinetics: lower loadings (5 wt%) optimized prokaryotic mitigation, reducing Escherichia coli by 88.90% and Staphylococcus aureus by 84.58%, whereas eukaryotic Candida albicans inhibition peaked at 70.21% under 15 wt% loading (CW-Q15) due to dense chitin-wall disruption. Under 15 wt% loading, film tensile strength shifted from 28.99 to 19.66 MPa and water permeability rose from 384.09 to 441.25 g/day m(2). Density Functional Theory (DFT) calculations validated optical sensitivity, showing a narrowed energy gap (E(g)) from 3.0748 to 2.7216 eV. Crucially, CW-Q15 functioned as a selective biosensor, showing target-specific fluorescent fingerprints: green dots for E. coli, green threads for S. aureus, and red holed circles for C. albicans. Real-world fresh-cut apple storage trials confirmed robust active preservation, offering a sustainable, biodegradable smart-packaging solution. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Ali, Eman AboBakr AU - Ali EA AD - Polymers and Pigments Department, Chemical Industries Research Institute, National Research Centre, 12622, Dokki, Giza, Egypt. Electronic address: eman.abobakr@outlook.com. FAU - El-Masry, Hossam M AU - El-Masry HM AD - Chemistry of Natural Microbial Products Department, National Research Centre, 33 El-Bohouth St., Former ElTahrir St., Dokki, P.O.12622, Giza, Egypt. Electronic address: drhossamnrc33@gmail.com. FAU - Tohamy, Hebat-Allah S AU - Tohamy HS AD - Cellulose and Paper Department, National Research Centre, 33 El Bohouth Str., P.O. 12622, Dokki, Giza, Egypt. Electronic address: hebasarhan89@yahoo.com. LA - eng PT - Journal Article DEP - 20261029 PL - Netherlands TA - Int J Biol Macromol JT - International journal of biological macromolecules JID - 7909578 SB - IM OTO - NOTNLM OT - Antimicrobial films OT - Apple preservation OT - Fluorescent zinc doped carbon quantum dots OT - Food spoilage indicator OT - Smart packaging OT - Whey protein isolate COIS- Declaration of competing interest There is no competing interest. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:29 PHST- 2026/04/28 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:29 [entrez] AID - S0141-8130(26)04961-5 [pii] AID - 10.1016/j.ijbiomac.2026.155009 [doi] PST - aheadofprint SO - Int J Biol Macromol. 2026 Oct 29:155009. doi: 10.1016/j.ijbiomac.2026.155009. PMID- 42956874 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-0003 (Electronic) IS - 0141-8130 (Linking) DP - 2026 Oct 29 TI - Structural characterization of longan polysaccharide and its modulation of gut microbiota and metabolic profiles. PG - 155105 LID - S0141-8130(26)05058-0 [pii] LID - 10.1016/j.ijbiomac.2026.155105 [doi] AB - The present work characterized the structure of longan polysaccharide (LP) and investigated its fermentation behavior and modulatory effects on pooled human gut microbiota. LP was composed predominantly of glucose with minor arabinose and galactose in molar ratios of 97.49%: 1.65%: 0.87%, with an average molecular weight of 7.34 x 10(7) g/mol (PDI 1.09). The LP main chain consists of -->6)-alpha-D-Glcp-(1--> linkages with sparse branches at O-3/O-4 of Glcp and O-2 of Galp. LP selectively enriched saccharolytic species including Bacteroides thetaiotaomicron, B. ovatus, Prevotella copri, and Parabacteroides distasonis that harbor GH97 and other co-enriched CAZyme families. These changes suggest a potential role in LP degradation and potential ABC/PTS-dependent uptake. Functionally, this selective microbiota modulation may drive enhanced short-chain fatty acid production and nitrogen metabolism, generating metabolites including GABA, taurine, and tryptophan/indole derivatives. Collectively, LP functions as a slow-fermentable, microbiota-targeted alpha-glucan that reshapes gut microbial metabolism toward SCFA and immune-relevant metabolite signature. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Wang, Shengwei AU - Wang S AD - School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, 529020, China; College of Chinese Medicine and Health, Guizhou University of Traditional Chinese Medicine, Guiyang, 550025, China. FAU - Yang, Qianming AU - Yang Q AD - School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, 529020, China. FAU - Chen, Xiaoyan AU - Chen X AD - School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, 529020, China. FAU - Liu, Hui AU - Liu H AD - College of Food Science, South China Agricultural University, Guangzhou, 510642, China. FAU - Wu, Rihui AU - Wu R AD - School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, 529020, China. Electronic address: wyuchemwrh@126.com. FAU - Yang, Ruili AU - Yang R AD - College of Food Science, South China Agricultural University, Guangzhou, 510642, China. FAU - Li, Wu AU - Li W AD - School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, 529020, China. Electronic address: leewuu@163.com. LA - eng PT - Journal Article DEP - 20261029 PL - Netherlands TA - Int J Biol Macromol JT - International journal of biological macromolecules JID - 7909578 SB - IM OTO - NOTNLM OT - CAZymes OT - Gut microbiota OT - Longan polysaccharide OT - Metabolomics OT - Metagenomics COIS- Declaration of competing interest The authors declared no conflict of interest. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:29 PHST- 2026/05/16 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/29 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:29 [entrez] AID - S0141-8130(26)05058-0 [pii] AID - 10.1016/j.ijbiomac.2026.155105 [doi] PST - aheadofprint SO - Int J Biol Macromol. 2026 Oct 29:155105. doi: 10.1016/j.ijbiomac.2026.155105. PMID- 42956871 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-0003 (Electronic) IS - 0141-8130 (Linking) DP - 2026 Oct 29 TI - Interactions between Hexokinase 2 and host proteins and their involvement in tissue tropism upon Hepatospora eriocheir infection in Eriocheir sinensis. PG - 155098 LID - S0141-8130(26)05051-8 [pii] LID - 10.1016/j.ijbiomac.2026.155098 [doi] AB - Hepatospora eriocheir is the causative pathogen of hepatopancreatic necrosis disease (HPND) in Eriocheir sinensis, and this microsporidian parasite specifically colonizes crab hepatopancreatic tissue. The molecular mechanisms underlying such tissue-specific infection remain largely unclear. Microsporidian parasites generally undergo extensive genomic degeneration, accompanied by the loss of oxidative phosphorylation pathways and most glycolysis-related genes. Nevertheless, H. eriocheir retains two paralogous hexokinase genes. Among them, HeHK2 exhibits relatively high sequence conservation but lacks detectable catalytic activity, indicating that this protein exerts non-enzymatic functions during pathogen infection. Immunofluorescence assays verified that H. eriocheir mainly resides in hepatopancreatic epithelial cells of crabs. Immunoprecipitation-Mass Spectrometry (IP- LC-MS/MS) was adopted to screen potential host proteins interacting with HeHK2. Three candidate proteins, including mitochondrial amidoxime reducing component (mARC), alpha-amylase 2-like protein and C-type lectin, were selected for subsequent co-immunoprecipitation validation based on their biological functions associated with host energy metabolism and innate immune regulation. Histological and ultrastructural observation further revealed mitochondrial aggregation and abnormal morphological changes in infected hepatopancreatic tissues. These interactions suggest a working model in which HeHK2 may contribute to hepatopancreas-specific infection by modulating mitochondria-associated host metabolism and innate immunity; however, direct functional evidence for this model requires further investigation. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Ding, Zhengfeng AU - Ding Z AD - Institute of Aquatic Biology and School of Geography, Jiangsu Second Normal University, 77 West Beijing Road, Nanjing, 210013, China. Electronic address: ding@jssnu.edu.cn. FAU - Wu, Yang AU - Wu Y AD - School of Life Sciences, Fudan University, Shanghai, 200438, China. FAU - Wu, Qilu AU - Wu Q AD - Institute of Aquatic Biology and School of Geography, Jiangsu Second Normal University, 77 West Beijing Road, Nanjing, 210013, China. FAU - Xu, Yini AU - Xu Y AD - Institute of Aquatic Biology and School of Geography, Jiangsu Second Normal University, 77 West Beijing Road, Nanjing, 210013, China. FAU - Shen, Yixin AU - Shen Y AD - Institute of Aquatic Biology and School of Geography, Jiangsu Second Normal University, 77 West Beijing Road, Nanjing, 210013, China. FAU - Zhang, Xinyu AU - Zhang X AD - Institute of Aquatic Biology and School of Geography, Jiangsu Second Normal University, 77 West Beijing Road, Nanjing, 210013, China. LA - eng PT - Journal Article DEP - 20261029 PL - Netherlands TA - Int J Biol Macromol JT - International journal of biological macromolecules JID - 7909578 SB - IM OTO - NOTNLM OT - Eriocheir sinensis OT - Hepatospora eriocheir OT - Hexokinase OT - Host-pathogen protein interaction OT - Innate immunity OT - Mitochondrial metabolism COIS- Declaration of competing interest The authors declare that the research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:29 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/27 00:00 [revised] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:29 [entrez] AID - S0141-8130(26)05051-8 [pii] AID - 10.1016/j.ijbiomac.2026.155098 [doi] PST - aheadofprint SO - Int J Biol Macromol. 2026 Oct 29:155098. doi: 10.1016/j.ijbiomac.2026.155098. PMID- 42956866 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-0003 (Electronic) IS - 0141-8130 (Linking) DP - 2026 Oct 29 TI - Lignocellulosic unidirectional natural fiber-reinforced thermoplastics (LUD-NFRTs): Sustainable biocomposites with superior longitudinal mechanical performance. PG - 155101 LID - S0141-8130(26)05054-3 [pii] LID - 10.1016/j.ijbiomac.2026.155101 [doi] AB - Lignocellulosic fibers, composed primarily of cellulose, hemicellulose, and lignin, have emerged as sustainable biological reinforcements for polymer composites because of their low density, renewability, and favorable specific mechanical properties. Among natural fiber-reinforced polymers, lignocellulosic unidirectional natural fiber-reinforced thermoplastics (LUD-NFRTs) arrange the renewable lignocellulosic fibers with a predominantly aligned architecture to promote longitudinal stiffness and strength for efficient direction-dependent load bearing. Nevertheless, their broader adoption remains constrained by the inherent drawbacks of natural fibers and by processing and scale-up challenges. This review examines how reinforcement form, thermoplastic matrix selection, interfacial modification, and manufacturing conditions govern the mechanical performance of LUD-NFRTs. Published studies are critically synthesized by linking reinforcement architecture, processing, and structural characteristics with direction-dependent mechanical behavior. The discussion covers longitudinal and transverse tensile properties, together with the available evidence for compressive, flexural, impact, and interlaminar responses. Strategies explored over the past two decades include fiber treatment, matrix modification, nanocellulose incorporation, fiber hybridization, and improvements in fiber arrangement and manufacturing processes. These advances highlight the potential of LUD-NFRTs as high-performance biocomposites for sustainable lightweight engineering. Further progress in preform design and preparation, composite defect and porosity control, off-axis mechanical performance, long-term durability, and industrial manufacturing scalability is needed to realize their broader application potential. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Ren, Kehao AU - Ren K AD - Center for Renewable Carbon, University of Tennessee, Knoxville, TN, 37996, USA; School of Natural Resources, University of Tennessee, Knoxville, TN, 37996, USA. FAU - Clarkson, Caitlyn AU - Clarkson C AD - Manufacturing Science Division, Oak Ridge National Laboratory, Knoxville, TN, 37830, USA. FAU - Zhao, Xianhui AU - Zhao X AD - Environmental Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA. FAU - Killingsworth, Laura Mae AU - Killingsworth LM AD - Manufacturing Science Division, Oak Ridge National Laboratory, Knoxville, TN, 37830, USA. FAU - Tekinalp, Halil AU - Tekinalp H AD - Manufacturing Science Division, Oak Ridge National Laboratory, Knoxville, TN, 37830, USA. FAU - Li, Kai AU - Li K AD - Buildings and Transportation Science Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA. FAU - Harper, David P AU - Harper DP AD - Center for Renewable Carbon, University of Tennessee, Knoxville, TN, 37996, USA; School of Natural Resources, University of Tennessee, Knoxville, TN, 37996, USA. FAU - Wang, Siqun AU - Wang S AD - Center for Renewable Carbon, University of Tennessee, Knoxville, TN, 37996, USA; School of Natural Resources, University of Tennessee, Knoxville, TN, 37996, USA. FAU - Vaidya, Uday AU - Vaidya U AD - Buildings and Transportation Science Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831, USA; Department of Mechanical and Aerospace Engineering, University of Tennessee, Knoxville, TN, 37996, USA. FAU - Ozcan, Soydan AU - Ozcan S AD - Manufacturing Science Division, Oak Ridge National Laboratory, Knoxville, TN, 37830, USA. FAU - Wang, Lu AU - Wang L AD - Center for Renewable Carbon, University of Tennessee, Knoxville, TN, 37996, USA; School of Natural Resources, University of Tennessee, Knoxville, TN, 37996, USA. Electronic address: luwang@utk.edu. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - Netherlands TA - Int J Biol Macromol JT - International journal of biological macromolecules JID - 7909578 SB - IM OTO - NOTNLM OT - Fiber/matrix interface OT - Lignocellulosic fibers OT - Manufacturing process OT - Mechanical properties OT - Natural fibers OT - Preform OT - Reinforcement architecture OT - Unidirectional thermoplastic composites COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:29 PHST- 2026/07/20 00:00 [received] PHST- 2026/10/20 00:00 [revised] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:29 [entrez] AID - S0141-8130(26)05054-3 [pii] AID - 10.1016/j.ijbiomac.2026.155101 [doi] PST - aheadofprint SO - Int J Biol Macromol. 2026 Oct 29:155101. doi: 10.1016/j.ijbiomac.2026.155101. PMID- 42956862 OWN - NLM STAT- Publisher LR - 20261029 IS - 1096-1186 (Electronic) IS - 1043-6618 (Linking) DP - 2026 Oct 29 TI - Effects of Probiotic Supplementation on Depression, Anxiety, and Sleep Quality in Healthy Participants: A Systematic Review and Meta-Analysis. PG - 109716 LID - S1043-6618(26)01631-2 [pii] LID - 10.1016/j.phrs.2026.109716 [doi] AB - This systematic review and meta-analysis evaluated the effects of probiotic supplementation on depression, anxiety, and sleep quality in healthy adults. PubMed, Cochrane Library, Web of Science, and Embase were searched from inception to September 18, 2026. Randomized controlled trials (RCTs) comparing probiotics with placebo for >/=4 weeks were included. Data from 20 studies involving 1,508 participants were analyzed using Stata 17.0. Random-effects or fixed-effects models were selected according to heterogeneity. The GRADE approach was used to assess evidence certainty. Probiotics significantly reduced depression scores (SMD = -0.23, 95% CI: -0.36 to -0.10, P < 0.01; I(2) = 4.67%) and improved sleep quality (SMD = -0.37, 95% CI: -0.52 to -0.22, P < 0.01; I(2) = 41.67%). However, the overall effect on anxiety was not significant (SMD = -0.11, 95% CI: -0.31 to 0.09, P = 0.27; I(2) = 61.41%). Subgroup analyses were exploratory and unadjusted; no definitive conclusion can be drawn regarding intervention duration or strain type. GRADE certainty was moderate for depression and sleep quality, but very low for anxiety. In conclusion, probiotic supplementation may alleviate depressive symptoms and improve sleep quality in healthy adults, whereas its effect on anxiety remains inconclusive. Larger, standardized RCTs are needed to confirm these findings and clarify strain-specific effects. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Liu, Qinhan AU - Liu Q AD - Department of Pharmacy, the Affiliated Hospital, Southwest Medical University, Luzhou, Sichuan Province, 646000, China; School of Pharmacy, Southwest Medical University, Luzhou, Sichuan Province, 646000, China. FAU - Zhang, Ling AU - Zhang L AD - Department of Pharmacy, the Affiliated Hospital, Southwest Medical University, Luzhou, Sichuan Province, 646000, China; School of Pharmacy, Southwest Medical University, Luzhou, Sichuan Province, 646000, China. FAU - Wang, Yaomin AU - Wang Y AD - Department of Pharmacy, the Affiliated Hospital, Southwest Medical University, Luzhou, Sichuan Province, 646000, China; School of Pharmacy, Southwest Medical University, Luzhou, Sichuan Province, 646000, China. FAU - Li, Jun AU - Li J AD - Department of Traditional Chinese Medicine, the Affiliated Hospital, Southwest Medical University, Luzhou, Sichuan Province, 646000, China. Electronic address: ljadoctor@swmu.edu.cn. FAU - Li, Yaling AU - Li Y AD - Department of Pharmacy, the Affiliated Hospital, Southwest Medical University, Luzhou, Sichuan Province, 646000, China. Electronic address: lylapothecary@swmu.edu.cn. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - Netherlands TA - Pharmacol Res JT - Pharmacological research JID - 8907422 SB - IM OTO - NOTNLM OT - Anxiety OT - Depression OT - Meta-analysis OT - Probiotics OT - Sleep quality COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Declaration of competing interest All authors declare that they have no conflict of interest. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:29 PHST- 2026/07/18 00:00 [received] PHST- 2026/10/22 00:00 [revised] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:29 [entrez] AID - S1043-6618(26)01631-2 [pii] AID - 10.1016/j.phrs.2026.109716 [doi] PST - aheadofprint SO - Pharmacol Res. 2026 Oct 29:109716. doi: 10.1016/j.phrs.2026.109716. PMID- 42956856 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-2976 (Electronic) IS - 0960-8524 (Linking) DP - 2026 Oct 29 TI - Hydrogen nanobubbles overcome metabolic bottlenecks and remodel transcriptional networks for high-rate methanogenesis in the anaerobic digestion of Paulownia leaf litter. PG - 136136 LID - S0960-8524(26)02218-2 [pii] LID - 10.1016/j.biortech.2026.136136 [doi] AB - Volatile fatty acid (VFA) accumulation limits anaerobic digestion (AD) of lignocellulosic biomass by causing acidification and microbial metabolic imbalance. Paulownia leaf litter (PLL), an underutilized lignocellulosic residue with potential for renewable energy recovery, was selected as the substrate. This study evaluated hydrogen nanobubble water (H(2)-NBW) as a strategy to alleviate VFA inhibition and investigated associated microbial responses using integrated multi-omics analyses. H(2)-NBW increased cumulative methane yields by 26.4-34.5% compared with controls, accelerated VFA turnover, improved acid-base stability and buffering capacity, and enhanced extracellular hydrolytic enzyme activities by 12.7-42.4%. Coenzyme F420 fluorescence intensity increased by 11.5-30.7%, indicating enhanced methanogenic activity. Amplicon sequencing suggested that H(2)-NBW enriched microorganisms associated with carbohydrate degradation and fermentation, while overall community restructuring remained limited. Metagenomic analysis revealed increased functional potential for lignocellulose degradation, reflected in carbohydrate-active enzyme and H(2) metabolism genes. Meta-transcriptomic analysis further showed increased transcription of genes associated with hydrolysis, acetate-forming metabolism, the Frh/Mvh-Hdr hydrogenase system, and hydrogenotrophic methanogenesis. These responses suggest that H(2)-NBW may support carbon conversion and hydrogenotrophic methanogenesis, accompanied by enhanced transcription of genes involved in substrate degradation, H(2) metabolism, and methane formation. The observed improvements may be associated with exogenous H(2) availability, microbial metabolic responses, and potential nanobubble-associated physicochemical effects. Overall, this study links improved AD performance with coordinated microbial functional and transcriptional responses, highlighting the potential of H(2)-NBW to improve lignocellulosic biomass conversion and methane recovery. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Fan, Yujie AU - Fan Y AD - Institute of Paulownia, Henan Agricultural University, Zhengzhou 450002, Henan, PR China. FAU - Guo, Dawei AU - Guo D AD - Institute of Paulownia, Henan Agricultural University, Zhengzhou 450002, Henan, PR China. FAU - He, Yueya AU - He Y AD - Institute of Paulownia, Henan Agricultural University, Zhengzhou 450002, Henan, PR China. FAU - Zhai, Xiaoqiao AU - Zhai X AD - Henan Academy of Forestry, Zhengzhou 450008, PR China. FAU - Zhao, Zhenli AU - Zhao Z AD - Institute of Paulownia, Henan Agricultural University, Zhengzhou 450002, Henan, PR China. FAU - Fan, Guoqiang AU - Fan G AD - Institute of Paulownia, Henan Agricultural University, Zhengzhou 450002, Henan, PR China. Electronic address: zlxx64@henau.edu.cn. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Bioresour Technol JT - Bioresource technology JID - 9889523 SB - IM OTO - NOTNLM OT - Anaerobic digestion OT - Hydrogennanobubble water OT - Methanogenesis OT - Paulownia leaf litter OT - Transcriptional responses OT - VFA inhibition COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:29 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:29 [entrez] AID - S0960-8524(26)02218-2 [pii] AID - 10.1016/j.biortech.2026.136136 [doi] PST - aheadofprint SO - Bioresour Technol. 2026 Oct 29:136136. doi: 10.1016/j.biortech.2026.136136. PMID- 42956849 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-2976 (Electronic) IS - 0960-8524 (Linking) DP - 2026 Oct 29 TI - Electrical Stimulation-driven Community Selection and Metabolic Allocation for Enhanced Polyhydroxyalkanoates Production with Improved Sludge Settleability. PG - 136159 LID - S0960-8524(26)02241-8 [pii] LID - 10.1016/j.biortech.2026.136159 [doi] AB - Low-cost production of polyhydroxyalkanoates (PHA) from organic wastes using mixed microbial cultures (MMC) is constrained by two persistent bottlenecks: insufficient enrichment of PHA-storing taxa and poor biomass settleability. This study evaluated whether low-voltage electrical stimulation could simultaneously address both limitations in aerobic dynamic feeding systems. Four reactors were operated at 0, 0.3, 0.6, and 0.9 V over feast-famine cycles. At 0.6 V, PHA content reached 48% of cell dry weight, versus 37% in the control, while the 30-minute sludge volume index decreased from 334 to 299 mL/g. Mechanistically, electrical stimulation is proposed to act through two coupled layers: it may enrich functional genera (Thauera, Paracoccus, and Sediminibacterium) at the community level, and may modulate carbon flux allocation in a voltage-dependent manner-moderate activation may favor PHA storage, whereas excessive voltage may direct carbon toward additional metabolic pathways beyond PHA. The settleability improvement was associated with LB-EPS remodeling, with increased hydrophobic protein-like components and decreased humic-like substances. These findings suggest that electrical stimulation may serve as a unified operational lever that addresses both bottlenecks in MMC-based PHA production, offering a potential practical pathway to streamline operations. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Wang, Mier AU - Wang M AD - College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China. FAU - Huang, Shihan AU - Huang S AD - College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China. FAU - Yue, Jiawei AU - Yue J AD - College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China. FAU - Ding, Yixiao AU - Ding Y AD - College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China. FAU - Chen, Xi AU - Chen X AD - College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China. FAU - Tang, Lin AU - Tang L AD - College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China. Electronic address: tanglin@hnu.edu.cn. FAU - Yuan, Jie AU - Yuan J AD - College of Environmental Science and Engineering, Hunan University, Changsha 410082, PR China; Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, PR China. Electronic address: yuanj@hnu.edu.cn. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Bioresour Technol JT - Bioresource technology JID - 9889523 SB - IM OTO - NOTNLM OT - Aerobic dynamic feeding (ADF) OT - Electrical stimulation OT - Mixed microbial cultures OT - Polyhydroxyalkanoates (PHA) COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:29 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/23 00:00 [revised] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:29 [entrez] AID - S0960-8524(26)02241-8 [pii] AID - 10.1016/j.biortech.2026.136159 [doi] PST - aheadofprint SO - Bioresour Technol. 2026 Oct 29:136159. doi: 10.1016/j.biortech.2026.136159. PMID- 42956845 OWN - NLM STAT- Publisher LR - 20261029 IS - 1872-9649 (Electronic) IS - 1568-1637 (Linking) DP - 2026 Oct 29 TI - Selective neuronal vulnerability in Alzheimer's disease: mechanisms, biomarkers, and therapeutic implications. PG - 103430 LID - S1568-1637(26)00422-8 [pii] LID - 10.1016/j.arr.2026.103430 [doi] AB - Alzheimer's disease (AD) is characterized by marked heterogeneity in the timing and extent of neuronal dysfunction and degeneration. Specific neuronal populations, including entorhinal cortex layer II stellate cells, hippocampal CA1 pyramidal neurons, basal forebrain cholinergic neurons, locus coeruleus noradrenergic neurons, and prefrontal parvalbumin interneurons, show different combinations of functional, pathological, and degenerative vulnerability during disease progression. The strength and nature of this evidence, however, vary across neuronal populations and between human studies and experimental models. Selective vulnerability is therefore unlikely to reflect a single cell-type-specific mechanism. Instead, pathological stress in AD interacts with intrinsic cellular properties, circuit organization, and the local microenvironment. Metabolic stress, axonal transport impairment, excitotoxicity and Ca(2)(+) dysregulation, neuroinflammation, lipid dysregulation and ferroptotic stress, and proteostasis failure may consequently produce distinct functional and degenerative outcomes across neuronal populations. Advances in transcriptomics, neuropathology, multimodal imaging, and circuit-level analyses are also identifying molecular, regional, and functional measures associated with early involvement of vulnerable neuronal systems, although validated cell-type-specific biomarkers remain limited. In this Review, we integrate these findings to examine the evidence for selective neuronal vulnerability, the mechanisms that may modify neuronal susceptibility, and their implications for biomarker development and therapy. We further consider how vulnerability-informed strategies could complement pathology-directed therapies by aligning therapeutic mechanisms with prominent cellular or circuit dysfunction and disease stage. This perspective may help explain the spatial and temporal heterogeneity of AD and guide more biologically informed approaches to early detection and intervention. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Tian-Yang, Xu AU - Tian-Yang X AD - Department of Pharmacology, Health Science Center, Ningbo University, Ningbo, Zhejiang 315211, China. FAU - Er-Deng, E AU - Er-Deng E AD - Department of Pharmacology, Health Science Center, Ningbo University, Ningbo, Zhejiang 315211, China. FAU - Ting, Zhang AU - Ting Z AD - Department of Pharmacology, Health Science Center, Ningbo University, Ningbo, Zhejiang 315211, China. FAU - Xiao-Qin, Zhang AU - Xiao-Qin Z AD - Department of Pharmacology, Health Science Center, Ningbo University, Ningbo, Zhejiang 315211, China. Electronic address: zhangxiaoqin1@nbu.edu.cn. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - Ageing Res Rev JT - Ageing research reviews JID - 101128963 SB - IM OTO - NOTNLM OT - Alzheimer's disease OT - biomarkers OT - circuit dysfunction OT - selective neuronal vulnerability OT - therapeutic strategies COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:29 PHST- 2026/05/04 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:29 [entrez] AID - S1568-1637(26)00422-8 [pii] AID - 10.1016/j.arr.2026.103430 [doi] PST - aheadofprint SO - Ageing Res Rev. 2026 Oct 29:103430. doi: 10.1016/j.arr.2026.103430. PMID- 42956824 OWN - NLM STAT- Publisher LR - 20261029 IS - 1525-6014 (Electronic) IS - 0148-0545 (Linking) DP - 2026 Oct 29 TI - Protective role of aprepitant in paracetamol-induced renal injury: biochemical and histopathological evidence. PG - 1-10 LID - 10.1080/01480545.2026.2754403 [doi] AB - Paracetamol (PARA) is a commonly used analgesic and antipyretic, but overdose can result in severe hepatotoxicity and nephrotoxicity. Aprepitant (APRE), a selective neurokinin-1 receptor (NK-1R) antagonist used clinically as an antiemetic, may offer protective effects against drug-induced organ toxicity. This study aimed to evaluate the renoprotective potential of APRE in a rat model of PARA-induced nephrotoxicity. In this study, 36 male Albino Wistar rats were randomly assigned to six groups: Healthy, PARA (2 g/kg), APRE (20 mg/kg), N-acetylcysteine (NAC) + PARA (140 mg/kg + 2 g/kg), APRE + PARA (10 mg/kg + 2 g/kg), and APRE + PARA (20 mg/kg + 2 g/kg). Twenty-four hours after PARA administration, tissue and blood samples were collected from all groups for biochemical and histopathological analyses. Serum BUN and creatinine levels increased in the PARA group, whereas they improved in the NAC and APRE groups. Renal superoxide dismutase (SOD) activity and glutathione (GSH) levels decreased, but NAC and APRE significantly improved these parameters. Moreover, while malondialdehyde (MDA) levels in kidney tissue increased in the PARA group, they were significantly reduced in the NAC and APRE groups. In addition, histopathological changes supported the biochemical findings. APRE may protect the kidneys against PARA-induced damage by reducing oxidative stress and improving antioxidant defenses. FAU - Gharbali, Farnaz AU - Gharbali F AUID- ORCID: 0009-0000-5997-115X AD - Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey. FAU - Pourakbar Farzami, Soheil AU - Pourakbar Farzami S AUID- ORCID: 0009-0003-2385-7154 AD - Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey. FAU - Bekmez, Huseyin AU - Bekmez H AUID- ORCID: 0000-0001-9518-0523 AD - Department of Pharmacology, Faculty of Medicine, Nisantasi University, Istanbul, Turkey. FAU - Abbas Kabiri, Danial AU - Abbas Kabiri D AUID- ORCID: 0009-0001-3162-0152 AD - Department of Histology and Embryology, Faculty of Medicine, Ataturk University, Erzurum, Turkey. FAU - Albayrak, Abdulmecit AU - Albayrak A AUID- ORCID: 0000-0002-1062-1965 AD - Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Drug Chem Toxicol JT - Drug and chemical toxicology JID - 7801723 SB - IM OTO - NOTNLM OT - Aprepitant OT - kidney OT - paracetamol OT - rat OT - toxicity EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 20:12 PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 20:12 [entrez] AID - 10.1080/01480545.2026.2754403 [doi] PST - aheadofprint SO - Drug Chem Toxicol. 2026 Oct 29:1-10. doi: 10.1080/01480545.2026.2754403. PMID- 42956799 OWN - NLM STAT- Publisher LR - 20261029 IS - 2567-689X (Electronic) IS - 0340-6245 (Linking) DP - 2026 Oct 29 TI - Glycosylation and Immunogenicity in Recombinant Proteins Acting on the FVIII/VWF-ADAMTS13 Axis. LID - 10.1055/a-2991-4946 [doi] AB - Recombinant protein therapeutics used in thrombosis and hemostasis exhibit diverse immunogenic and pharmacokinetic behaviors, much of which can be attributed to host cell-dependent glycosylation. Glycan structures influence clearance, receptor recognition, antigen processing, and immune tolerance in protein specific ways. Factor VIII (FVIII), which also functions as a regulator that promotes ADAMTS13‑mediated cleavage of von Willebrand factor (VWF), represents a paradigm in which differences in glycan processing among host cell lines contribute to inhibitor formation by modulating antigen uptake and presentation. In contrast, VWF demonstrates a distinct model in which terminal sialylation and antennary branching primarily regulate clearance rather than immunogenicity; CHO derived recombinant VWF, enriched in alpha2 3 sialylation, shows reduced ASGPR mediated hepatic uptake and prolonged half life. ADAMTS13 presents a third, clinically unique case. Its multidomain architecture and dependence on N glycans for folding and epitope shielding may render it structurally vulnerable, and the absence of alpha2 6 sialylation in CHO derived rADAMTS13 may impair CD22 mediated B cell tolerance, potentially predisposing patients with congenital thrombotic thrombocytopenic purpura to alloantibody formation. Recent clinical observations underscore the need for long term immunogenicity monitoring. Collectively, these examples highlight glycosylation as a protein specific regulatory system that shapes both immunogenicity and pharmacokinetics. Future development of recombinant therapeutics targeting the VWF-ADAMTS13 axis will require optimized host cell selection, advanced glycoengineering, non immunogenic half life extension technologies, and integrated immunogenicity prediction models. Understanding and controlling glycosylation will be central to designing safer and more effective recombinant protein therapies in thrombosis and hemostasis. CI - Thieme. All rights reserved. FAU - Sakurai, Yoshihiko AU - Sakurai Y AD - Nara Medical University, Department of Legal Medicine, Nara, Japan, Kashihara. FAU - Fujimura, Yoshihiro AU - Fujimura Y AD - Japanese Red Cross Kinki Block Blood Center, 2. Department of Research and Development, Osaka, Japan, Ibaraki. AD - Nara Medical University, Department of Blood Transfusion Medicine, Nara, Japan, Kashihara. FAU - Sakai, Kazuya AU - Sakai K AD - Nara Medical University, Department of Blood Transfusion Medicine, Nara, Japan, Kashihara. FAU - Kan, Akihiko AU - Kan A AD - Kainos Laboratories Inc, Kasama Laboratory, Ibaraki, Japan, Kasama. FAU - Kasuda, Shogo AU - Kasuda S AD - Nara Medical University, Department of Legal Medicine, Nara, Japan, Kashihara. FAU - Matsui, Taei AU - Matsui T AD - Fujita Health University School of Medical Sciences, Faculty of Medical Technology, Aichi, Japan, Toyoake. LA - eng PT - Journal Article DEP - 20261029 PL - Germany TA - Thromb Haemost JT - Thrombosis and haemostasis JID - 7608063 SB - IM COIS- Yoshihiro Fujimura invented the ELISA assay used to assess ADAMTS13 activity and is a scientific advisor to Kainos and Sysmex. Kazuya Sakai has received speaker fees from Sanofi and Takeda. Akihiko Kan is an employee of Kainos. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:53 PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:53 [entrez] AID - 10.1055/a-2991-4946 [doi] PST - aheadofprint SO - Thromb Haemost. 2026 Oct 29. doi: 10.1055/a-2991-4946. PMID- 42956788 OWN - NLM STAT- Publisher LR - 20261029 IS - 2665-9913 (Electronic) IS - 2665-9913 (Linking) DP - 2026 Oct 29 TI - Long-term evaluation of the effectiveness and safety of autologous haematopoietic stem-cell transplantation for systemic sclerosis (NISSC1-LTE): a cohort study. LID - S2665-9913(26)00227-4 [pii] LID - 10.1016/S2665-9913(26)00227-4 [doi] AB - BACKGROUND: Systemic sclerosis is a rare and heterogeneous chronic autoimmune disease. It has the highest mortality of the rheumatic diseases and few treatment options exist. Autologous haematopoietic stem-cell transplantation (HSCT) is an effective and established therapeutic option in selected patients with early severe systemic sclerosis. This study aimed to evaluate the long-term safety and effectiveness of autologous HSCT in patients with systemic sclerosis in real-life clinical practice. METHODS: This cohort study (NISSC1-LTE) followed up patients with systemic sclerosis included in the prospective European Society for Blood and Marrow Transplantation NISSC1 study who had undergone their first autologous HSCT for severe progressive systemic sclerosis. Follow-up data were collected from 13 participating centres in France, Germany, Netherlands, Italy, Spain, Switzerland, and Brazil. Participating centres completed a yearly case report form for each patient alive after 2 years and up to 10 years after autologous HSCT. The primary endpoint was progression-free survival, defined as survival from baseline without evidence of systemic sclerosis progression. Late complications after autologous HSCT, including malignancies, secondary autoimmune diseases, infections, and major adverse cardiovascular events were also analysed. People with lived experience of systemic sclerosis were involved in the design and conduct of this study. The NISSC1 study is registered with ClinicalTrials.gov (NCT02516124) and is complete. FINDINGS: All 80 patients with systemic sclerosis who had an autologous HSCT between Dec 4, 2012, and Jan 19, 2016, were included in this long-term extension study. 12 patients were lost to follow-up, including five patients with a follow-up less than 5 years. Median age at autologous HSCT was 43 years (IQR 33-51), 57 (71%) of 80 patients were female, 23 (29%) were male, and median follow-up was 9.2 years (IQR 8.9-9.6) after autologous HSCT. Progression-free survival was 81% (95% CI 71-88) at 2 years, 72% (61-81) at 5 years, and 60% (47-71) at 10 years. 18 patients died during the study; causes of death were autologous HSCT-related in five (6%) of 80 patients, disease progression in nine (11%) patients, post-autologous HSCT malignancy in two (3%) patients, and late serious infection in two (3%) patients. 34 (43%) of 80 patients maintained remission without immunosuppressive treatment over 10 years. INTERPRETATION: This study provides long-term follow-up data after autologous HSCT in patients with systemic sclerosis allowing robust assessment of response rate, late complications, and sustained treatment benefit. The extended timeframe might serve as an important comparator for all upcoming, cellular treatment options. FUNDING: None. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY 4.0 license. Published by Elsevier Ltd.. All rights reserved. FAU - Henes, Joerg AU - Henes J AD - Centre for Interdisciplinary Clinical Immunology, Rheumatology and Autoimmune Diseases and Department of Internal Medicine II (Oncology, Haematology, Immunology and Rheumatology), University Hospital Tubingen, Tubingen, Germany. FAU - Oliveira, Maria Carolina AU - Oliveira MC AD - Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Brazil. FAU - Labopin, Myriam AU - Labopin M AD - European Society for Blood and Marrow Transplantation Paris Study Office, Paris, France; Department of Haematology, INSERM UMR 938, Saint Antoine Hospital, Universite Pierre et Marie Curie, Paris, France. FAU - Badoglio, Manuela AU - Badoglio M AD - European Society for Blood and Marrow Transplantation Paris Study Office, Paris, France. FAU - Scherer, Hans U AU - Scherer HU AD - Department of Rheumatology, Leiden University Medical Center, Leiden, Netherlands. FAU - Del Papa, Nicoletta AU - Del Papa N AD - Department of Rheumatology, Scleroderma Clinic, Ospedale G Pini, Milan, Italy. FAU - Daikeler, Thomas AU - Daikeler T AD - Department of Rheumatology, University and University Hospital of Basel, Basel, Switzerland. FAU - Schmalzing, Marc AU - Schmalzing M AD - Department of Rheumatology/Immunology, University Hospital of Wuerzburg, Wuerzburg, Germany. FAU - Schroers, Roland AU - Schroers R AD - Klinik fur Hamatologie, Onkologie, Stammzelltransplantation und Zelltherapie, Universitatsklinikum Bochum, Bochum, Germany. FAU - Martin, Thierry AU - Martin T AD - Service de Medecine Interne et Immunologie Clinique, Hopital Civil, Strasbourg, France. FAU - Pugnet, Gregory AU - Pugnet G AD - Service de Medecine Interne, CHU de Toulouse, Hopital Purpan, Toulouse, France. FAU - Marijt, Erik W A AU - Marijt EWA AD - Department of Hematology, Leiden University Medical Center, Leiden, Netherlands. FAU - Espinosa, Gerard AU - Espinosa G AD - Department of Autoimmune Diseases, Hospital Clinic of Barcelona, Barcelona, Spain. FAU - Rovira, Montserrat AU - Rovira M AD - Department of Haematology, Hospital Clinic Barcelona, Barcelona, Spain. FAU - Greco, Raffaella AU - Greco R AD - Unit of Hematology and Bone Marrow Transplantation, IRCCS San Raffaele Scientific Institute, Vita-Salute San Raffaele University, Milan, Italy. FAU - Alexander, Tobias AU - Alexander T AD - Department of Rheumatology and Clinical Immunology, Charite - Universitatsmedizin Berlin, German Rheumatology Research Center, Berlin, Germany. FAU - Onida, Francesco AU - Onida F AD - Fondazione IRCCS Ca Granda Ospedale Maggiore Policlinico, University of Milan, Milan, Italy. FAU - Marjanovic, Zora AU - Marjanovic Z AD - Department of Haematology, Saint Antoine Hospital, AP-HP Assistance Publique Hopitaux de Paris, Paris, France. FAU - Farge, Dominique AU - Farge D AD - Centre de Reference des Maladies Auto-Immunes Systemiques Rares d'Ile-de-France, AP-HP, IRSL, INSERM U1342, Hopital St-Louis, Paris-Cite University, Paris, France; Department of Medicine, McGill University, Montreal, QC, Canada. Electronic address: Dominique.farge@inserm.fr. CN - Autoimmune Disease Working Party of the European Society for Blood and Marrow Transplantation LA - eng SI - ClinicalTrials.gov/NCT02516124 PT - Journal Article DEP - 20261029 PL - England TA - Lancet Rheumatol JT - The Lancet. Rheumatology JID - 101765308 SB - IM COIS- Declaration of interests JH has received speakers fees from AbbVie, AstraZeneca, Boehringer Ingelheim, Bristol Myers Squibb (BMS), Johnson & Johnson, Lilly, Novartis, Pfizer, Roche, SOBI, and UCB; travel support from AbbVie, UCB, BMS, Lilly, and Boehringer Ingelheim; and has participated in advisory boards for AbbVie, AstraZeneca, Boehringer Ingelheim, BMS, Johnson & Johnson, Novartis, SOBI, and UCB. RG has received speakers fees from Biotest, Pfizer, Medac, Qiagen, Kyverna, and Magenta; and has participated in advisory boards for Pfizer, BMS, and Merck Sharp & Dohme. TA has received speakers fees from AbbVie, Amgen, AstraZeneca, and GSK; and received a grant from Johnson & Johnson. DF has participated in a data safety monitoring board and advisory board for BMS. All other authors declare no competing interests. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:52 PHST- 2026/03/31 00:00 [received] PHST- 2026/07/09 00:00 [revised] PHST- 2026/07/09 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:52 [entrez] AID - S2665-9913(26)00227-4 [pii] AID - 10.1016/S2665-9913(26)00227-4 [doi] PST - aheadofprint SO - Lancet Rheumatol. 2026 Oct 29:S2665-9913(26)00227-4. doi: 10.1016/S2665-9913(26)00227-4. PMID- 42956785 OWN - NLM STAT- Publisher LR - 20261029 IS - 2468-2667 (Electronic) DP - 2026 Oct 29 TI - Prevalence and spectrum of skin diseases in adults in China: a nationwide cross-sectional study. LID - S2468-2667(26)00126-X [pii] LID - 10.1016/S2468-2667(26)00126-X [doi] AB - BACKGROUND: Skin diseases have been identified as a public health priority at the 78th World Health Assembly, with a call for strengthened national surveillance and evidence-based responses. However, in China, comprehensive national epidemiological data on the prevalence of skin diseases have been lacking. We aimed to bridge this data gap by conducting the China Skin Health study, a large-scale, population-based, cross-sectional study. METHODS: In this study, we used a multistage stratified cluster random sampling design to obtain a nationally representative sample of adults aged 18 years or older from diverse geographical regions of China. We collaborated with provincial centres for disease control and prevention to establish a survey team comprising public health workers and ten to 12 certified dermatologists in each of the five provinces selected for the study. Residents' committees disseminated study information through posters and WeChat groups to recruit participants, and data were collected at local community health centres. Standardised electronic questionnaires were administered by trained interviewers to collect information on demographic and other characteristics. Certified dermatologists examined sun-exposed areas (eg, scalp, face, neck, and extremities) in all participants, as well as unexposed areas reported as concerns by respondents, for common skin diseases spanning 12 categories and more than 60 specific conditions. Clinical diagnoses were made on the basis of observed skin manifestations. The total number of skin conditions was recorded for each participant, and the mean number of skin conditions was calculated. Point prevalence of skin diseases was defined as the proportion of participants with a dermatologist-confirmed diagnosis of any listed condition at the time of the survey. Sampling weights derived from the study sampling framework and the 2020 National Population Census were applied when calculating prevalence and conducting logistic regression analyses. The absolute number of dermatological cases nationwide was estimated by applying prevalence estimates to the 2020 Chinese census data. FINDINGS: From July 1, 2022, to June 27, 2024, 153 593 participants completed the survey and were included in the analysis. The study population had a mean age of 48.5 years (SD 16.8), with 75 512 (49.2%) men and 78 081 (50.8%) women; 98 926 (64.4%) participants resided in urban areas. The age-standardised prevalence of skin diseases was 55.6% (95% CI 51.2-59.9), corresponding to an estimated 618 million (95% CI 567-666) affected adults in China, with a mean of 0.98 (SD 1.22) skin diseases per person. Prevalence increased with age, from 42.0% (95% CI 35.3-48.6) in those aged 18-29 years to 79.5% (75.8-83.1) in those aged 80 years or older (p<0.0001 for trend). Skin diseases were more common in women (56.5%, 51.8-61.1) than in men (54.6%, 50.5-58.7; p=0.023) and more prevalent in southern China (62.5%, 56.2-68.9) than in northern China (47.4%, 43.2-51.7; p<0.0001). Benign cutaneous tumours were the most prevalent type of condition (31.1%, 28.7-33.5), followed by superficial fungal infections (15.0%, 12.5-17.5); dermatitis, eczema, and allergic dermatoses (9.0%, 7.8-10.1); pigmentary dermatoses (7.9%, 6.3-9.6); and sebaceous and sweat gland dermatoses (5.7%, 5.0-6.4). Factors associated with skin diseases included female sex, increasing age, residence in southern China, current or former smoking, current or former alcohol consumption, overweight or obesity, a history of allergies, and a history of photosensitivity. Despite the substantial burden, only 5.8% (4.5-7.3) of affected individuals had sought medical care, and 18.2% (16.7-19.9) had used over-the-counter medications in the past year. INTERPRETATION: Skin diseases could affect more than half of Chinese adults. These findings highlight a substantial unmet need and should inform national priorities. FUNDING: National Key R&D Program of China, Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences, National Natural Science Foundation of China, and Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY-NC 4.0 license. Published by Elsevier Ltd.. All rights reserved. FAU - Li, Jing AU - Li J AD - Hospital for Skin Diseases, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China. FAU - Jin, Hui AU - Jin H AD - Hospital for Skin Diseases, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China; Key Laboratory of Basic and Translational Research on Immune-Mediated Skin Diseases, Chinese Academy of Medical Sciences, Nanjing, China. FAU - Zhang, Ying AU - Zhang Y AD - Hospital for Skin Diseases, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China; Key Laboratory of Basic and Translational Research on Immune-Mediated Skin Diseases, Chinese Academy of Medical Sciences, Nanjing, China; Department of Infection Control and Public Health, The Second Affiliated Hospital of Jiaxing University, Jiaxing, China. FAU - Zhou, Peimei AU - Zhou P AD - The Second People's Hospital of Chengdu, Sichuan University, Chengdu, China. FAU - Hu, Yaoda AU - Hu Y AD - Department of Epidemiology and Biostatistics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China. FAU - Zhang, Lianhua AU - Zhang L AD - Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, China. FAU - Jin, Guangjie AU - Jin G AD - Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, China. FAU - Ding, Yan AU - Ding Y AD - Hainan Fifth People's Hospital, Hainan Skin Disease and Plastic Surgery Hospital, Haikou City, China. FAU - Fu, Huibing AU - Fu H AD - Hainan Fifth People's Hospital, Hainan Skin Disease and Plastic Surgery Hospital, Haikou City, China. FAU - Wang, Yaofei AU - Wang Y AD - Shaanxi Provincial Center for Disease Control and Prevention, Xi'an, China. FAU - Wang, Guan AU - Wang G AD - Shaanxi Provincial Center for Disease Control and Prevention, Xi'an, China. FAU - Lv, Chengzhi AU - Lv C AD - Dalian Dermatosis Hospital, Dalian, China. FAU - Jin, Dakun AU - Jin D AD - Dalian Dermatosis Hospital, Dalian, China. FAU - Chen, Tao AU - Chen T AD - The Second People's Hospital of Chengdu, Sichuan University, Chengdu, China. FAU - Nie, Jianjun AU - Nie J AD - The Second People's Hospital of Chengdu, Sichuan University, Chengdu, China. FAU - Chen, Yan AU - Chen Y AD - The Second People's Hospital of Chengdu, Sichuan University, Chengdu, China. FAU - Peng, Qiao AU - Peng Q AD - The Second People's Hospital of Chengdu, Sichuan University, Chengdu, China. FAU - Yang, Yihui AU - Yang Y AD - Sichuan Provincial Center for Disease Control and Prevention, Chengdu, China. FAU - Yu, Meiwen AU - Yu M AD - Hospital for Skin Diseases, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China. FAU - Wang, Hongsheng AU - Wang H AD - Hospital for Skin Diseases, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China. FAU - Lu, Yonghong AU - Lu Y AD - The Second People's Hospital of Chengdu, Sichuan University, Chengdu, China. FAU - Shan, Guangliang AU - Shan G AD - Department of Epidemiology and Biostatistics, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, China. FAU - Lu, Qianjin AU - Lu Q AD - Hospital for Skin Diseases, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China; Key Laboratory of Basic and Translational Research on Immune-Mediated Skin Diseases, Chinese Academy of Medical Sciences, Nanjing, China; Hunan Key Laboratory of Medical Epigenomics, Changsha, China. Electronic address: qianlu5860@pumcderm.cams.cn. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Lancet Public Health JT - The Lancet. Public health JID - 101699003 SB - IM COIS- Declaration of interests The authors declare no competing interests. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:52 PHST- 2026/01/14 00:00 [received] PHST- 2026/04/23 00:00 [revised] PHST- 2026/06/04 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:52 [entrez] AID - S2468-2667(26)00126-X [pii] AID - 10.1016/S2468-2667(26)00126-X [doi] PST - aheadofprint SO - Lancet Public Health. 2026 Oct 29:S2468-2667(26)00126-X. doi: 10.1016/S2468-2667(26)00126-X. PMID- 42956783 OWN - NLM STAT- Publisher LR - 20261029 IS - 2666-5247 (Electronic) IS - 2666-5247 (Linking) DP - 2026 Oct 29 TI - Novel dengue serotype 4 live virus human challenge: a phase 1 dose optimisation and homologous rechallenge study. PG - 101542 LID - S2666-5247(26)00197-7 [pii] LID - 10.1016/j.lanmic.2026.101542 [doi] AB - BACKGROUND: Dengue live virus human challenges (LVHCs) are key in dengue vaccine and pharmacological development, to down-select candidates and provide a useful platform for understanding viral-host immunology in a controlled setting. The primary aim of this study was to assess the safety, reactogenicity, and immunogenicity of a newly derived underattenuated dengue virus serotype-4-LVHC (DENV-4-LVHC) strain intended for future dengue challenge studies. METHODS: We conducted a first-in-human, phase 1, non-randomised, open-label study that evaluated the safety and optimised dose of the DENV-4-LVHC strain H-241 in flavivirus-naive healthy adult volunteers. The trial was conducted between April 28, 2022, and Dec 18, 2023, at the University of Maryland (Baltimore, MD, USA) and included a dose-optimisation study in which participants were enrolled into staggered-start, dose-ranging cohorts, beginning with the lowest dose, and homologous rechallenge substudy in which willing, primary-infected participants were rechallenged with the optimised dose of DENV-4 at least 12 months from the primary challenge. Successful challenge was defined as more than 90% participants developing self-limiting mild-to-moderate dengue symptoms with detectable RNAemia. Participants were analysed on the basis of the treatment group (primary infection vs rechallenge). This trial is registered with ClinicalTrials.gov (Identifier: NCT05268302). FINDINGS: 14 healthy adult participants were enrolled between March 31, 2022, and June 9, 2022 (dose optimisation), and were challenged with a subcutaneous dose of DENV-4-LVHC on June 12, 2023 (rechallenge). The majority were men (11 [79%] of 14), and almost half were non-White (6 [43%]). The mean participant age was 33.6 years (range 20-48). All 14 participants developed viraemia and symptoms consistent with self-limited, mild-to-moderate dengue at an optimised dose of 95 plaque-forming units (low dose). Six (60%) participants (of the original ten from the 2022 cohort) were rechallenged using the optimised dose 1 year after primary challenge, and none developed detectable dengue. Robust antibody responses, plasmablast expansion, and antiviral gene upregulation were seen during primary infection but not after rechallenge. No severe dengue, need for inpatient observation, or dengue challenge-related serious adverse events were observed. INTERPRETATION: DENV-4-LVHC was found to be safe and highly predictable for use in future challenge studies, to evaluate vaccine and therapeutic candidates. Immunity from previous DENV-4-LVHC infection conferred protection following homologous rechallenge 1 year after primary exposure. FUNDING: Military Infectious Disease Research Program; Medical Technology Enterprise Consortium. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved. FAU - Chua, Joel V AU - Chua JV AD - Institute for Human Virology, University of Maryland School of Medicine, Baltimore, MD, USA. Electronic address: jchua@ihv.umaryland.edu. FAU - Price, Angie AU - Price A AD - Institute for Human Virology, University of Maryland School of Medicine, Baltimore, MD, USA. FAU - Sharaf, Salma AU - Sharaf S AD - Institute for Human Virology, University of Maryland School of Medicine, Baltimore, MD, USA. FAU - Camacho Ortega, Sigrid AU - Camacho Ortega S AD - Institute for Human Virology, University of Maryland School of Medicine, Baltimore, MD, USA. FAU - Bernal, Paula J AU - Bernal PJ AD - Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, USA. FAU - Butler, Lauryn AU - Butler L AD - Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, USA. FAU - Koudjra, Abra Rachida AU - Koudjra AR AD - Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, USA. FAU - Joshi, Sudhaunshu AU - Joshi S AD - Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, USA. FAU - Hardy, Celine S C AU - Hardy CSC AD - Department of Microbiology and Immunology, State University of New York Upstate Medical University, Syracuse, NY, USA. FAU - Currier, Jeffrey R AU - Currier JR AD - Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA. FAU - Waickman, Adam T AU - Waickman AT AD - Department of Microbiology and Immunology, State University of New York Upstate Medical University, Syracuse, NY, USA. FAU - Cairo, Cristiana AU - Cairo C AD - Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA. FAU - Friberg, Heather AU - Friberg H AD - Viral Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA. FAU - Edelman, Robert AU - Edelman R AD - Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, USA. FAU - Lyke, Kirsten E AU - Lyke KE AD - Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, MD, USA. LA - eng SI - ClinicalTrials.gov/NCT05268302 PT - Journal Article DEP - 20261029 PL - England TA - Lancet Microbe JT - The Lancet. Microbe JID - 101769019 SB - IM COIS- Declaration of interests JVC and KEL have served on the Island Pharmaceutical Safety Monitoring Committee; additionally, KEL has served as chair in the TB31F Safety Committee and received travel support from the European Society of Clinical Microbiology and Infectious Diseases. RE reports paid consulting and serving as a member of the Data Safety Monitoring Committee for Takeda Vaccines. All other authors declare no competing interests. The views expressed in this manuscript reflect the results of research conducted by the authors and do not necessarily reflect the official policy or position of the Defense Health Agency, US Army, Department of War, or US Government. The study protocol was approved by the applicable Institutional Review Board in compliance with all applicable Federal regulations governing the protection of human subjects. The material has been reviewed by the Walter Reed Army Institute of Research, with no objection to its presentation or publication. The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or as reflecting true views of the US Department of the Army or US Department of Defense. The investigators have adhered to the policies for the protection of human subjects as prescribed in AR 70-25. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:52 PHST- 2026/01/29 00:00 [received] PHST- 2026/08/13 00:00 [revised] PHST- 2026/08/31 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:52 [entrez] AID - S2666-5247(26)00197-7 [pii] AID - 10.1016/j.lanmic.2026.101542 [doi] PST - aheadofprint SO - Lancet Microbe. 2026 Oct 29:101542. doi: 10.1016/j.lanmic.2026.101542. PMID- 42956782 OWN - NLM STAT- Publisher LR - 20261029 IS - 2666-5247 (Electronic) IS - 2666-5247 (Linking) DP - 2026 Oct 29 TI - Clinical and immunological correlates of CXCR4 coreceptor use in people with HIV-1 receiving antiretroviral therapy in the Netherlands: a multicentre cohort study. PG - 101518 LID - S2666-5247(26)00173-4 [pii] LID - 10.1016/j.lanmic.2026.101518 [doi] AB - BACKGROUND: CXCR4-using (X4) viruses are associated with faster disease progression in untreated HIV-1 infection, although their clinical, virological, and immunological correlates during suppressive antiretroviral therapy are not well characterised. We aimed to assess the associations between X4 coreceptor use and clinical outcomes, HIV persistence, and immunological profiles in people with HIV-1 receiving suppressive antiretroviral therapy. METHODS: In this multicentre cohort study, asymptomatic adults with HIV receiving suppressive antiretroviral therapy were enrolled from four HIV treatment centres in the Netherlands between Oct 16, 2019, and Oct 27, 2021 and followed up for 2 years. Clinical data were obtained from structured questionnaires and electronic health records. HIV-1 coreceptor use was predicted from triplicate env sequencing with Geno2pheno, and participants were classified as having CCR5-using (R5), X4, or mixed virus. HIV-1 subtype was assigned using env and pol sequences. Total and intact HIV DNA in CD4(+) T cells were quantified using multiplex digital PCR. Plasma proteomics, bulk peripheral blood mononuclear cell transcriptomics, and high-dimensional flow cytometry were used for systems-level profiling. Associations between predicted coreceptor use and clinical outcomes, HIV-reservoir measures, and immunological profiles were assessed using adjusted regression models and discovery and validation cohorts. FINDINGS: Coreceptor use was assigned in 1724 participants; 1222 had R5 virus, 336 had X4 virus, and 166 had mixed classifications. Primary clinical analyses included 1494 participants, of whom 1172 were in the R5 group and 322 were in the X4 group. Compared with the R5 group, the X4 group had a lower CD4(+) nadir, a higher prevalence of previous AIDS-defining events, poorer immunological recovery, and more comorbidities, particularly type 2 diabetes and non-affective psychiatric disorders. The median total HIV DNA was 1051 copies per 10(6) CD4(+) T cells in the X4 group versus 599 copies per 10(6) CD4(+) T cells in the R5 group, and the median intact HIV DNA was 23 copies per 10(6) CD4(+) T cells versus 15 copies per 10(6) CD4(+) T cells. No significant differences were observed in plasma inflammatory proteins. Participants in the X4 group had higher frequencies of activated CD4(+) T cells and PD-1(+) regulatory T cells, and transcriptomic analyses identified downregulation of two validated transcripts involved in transcriptional repression and lipid hydrolysis. INTERPRETATION: Among people with HIV receiving suppressive antiretroviral therapy, infection with X4 virus was associated with markers of more advanced previous HIV disease, impaired immune recovery, larger HIV reservoirs, and selected comorbidities despite viral suppression. Coreceptor use might therefore help to contextualise clinical, virological, and immunological heterogeneity in treated HIV-1 infection and could be relevant to future HIV cure studies. FUNDING: ViiV Healthcare. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved. FAU - Mehta, Kavita AU - Mehta K AD - HIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University Hospital, Ghent University, Ghent, Belgium. FAU - Otten, Twan AU - Otten T AD - Department of Internal Medicine and Radboud Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, Netherlands. FAU - Rios-Vazquez, Victoria AU - Rios-Vazquez V AD - Department of Internal Medicine and Radboud Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, Netherlands. FAU - Delporte, Mareva AU - Delporte M AD - HIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University Hospital, Ghent University, Ghent, Belgium. FAU - Verschoore, Maxime AU - Verschoore M AD - HIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University Hospital, Ghent University, Ghent, Belgium. FAU - Blomme, Evy E AU - Blomme EE AD - HIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University Hospital, Ghent University, Ghent, Belgium. FAU - Rokx, Casper AU - Rokx C AD - Department of Internal Medicine and Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Center, Rotterdam, Netherlands. FAU - Stalenhoef, Janneke AU - Stalenhoef J AD - Department of Internal Medicine and Infectious Diseases, Onze Lieve Vrouwe Gasthuis, Amsterdam, Netherlands. FAU - Berrevoets, Marvin A H AU - Berrevoets MAH AD - Department of Internal Medicine and Infectious Diseases, Elisabeth-TweeSteden Hospital, Tilburg, Netherlands. FAU - van Lunzen, Jan AU - van Lunzen J AD - Department of Internal Medicine and Radboud Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, Netherlands. FAU - Netea, Mihai G AU - Netea MG AD - Department of Internal Medicine and Radboud Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, Netherlands; Department of Immunology and Metabolism, Life and Medical Sciences Institute, University of Bonn, Bonn, Germany. FAU - Gerlo, Sarah AU - Gerlo S AD - HIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University Hospital, Ghent University, Ghent, Belgium. FAU - van der Ven, Andre J AU - van der Ven AJ AD - Department of Internal Medicine and Radboud Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, Netherlands. FAU - Vandekerckhove, Linos AU - Vandekerckhove L AD - HIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University Hospital, Ghent University, Ghent, Belgium. Electronic address: Linos.vandekerckhove@ugent.be. FAU - Rutsaert, Sofie AU - Rutsaert S AD - HIV Cure Research Center, Department of Internal Medicine and Pediatrics, Ghent University Hospital, Ghent University, Ghent, Belgium. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Lancet Microbe JT - The Lancet. Microbe JID - 101769019 SB - IM COIS- Declarations of interests KM and MD received New Investigator Scholarships to attend the Conference on Retroviruses and Opportunistic Infections (CROI), organised by the International Antiviral Society-USA in partnership with the CROI Foundation that covered conference registration and travel. KM and VR-V received institutional funding from ViiV Healthcare for the 2000HIV cohort study. CR received institutional grants and travel support from Gilead and ViiV Healthcare, has served on advisory boards for ViiV Healthcare, Gilead, and MSD, and holds shares in Immunocore and Eli Lilly. JvL was employed by ViiV Healthcare until Oct 31, 2022. LV received institutional funding and grants from ViiV Healthcare, institutional grants from Gilead, consulting fees from ViiV Healthcare, Gilead, and MSD, honoraria for lectures from ViiV Healthcare, and payment for expert testimony from ViiV Healthcare, Gilead, and MSD. All other authors declare no competing interests. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:52 PHST- 2025/11/24 00:00 [received] PHST- 2026/07/01 00:00 [revised] PHST- 2026/07/21 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:52 [entrez] AID - S2666-5247(26)00173-4 [pii] AID - 10.1016/j.lanmic.2026.101518 [doi] PST - aheadofprint SO - Lancet Microbe. 2026 Oct 29:101518. doi: 10.1016/j.lanmic.2026.101518. PMID- 42956773 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1474-547X (Electronic) IS - 0140-6736 (Linking) VI - 408 IP - 10566 DP - 2026 Oct 31 TI - Men's health: an intersectional and multilevel framework for understanding syndemic patterns of health. PG - 1866-1880 LID - S0140-6736(26)01489-3 [pii] LID - 10.1016/S0140-6736(26)01489-3 [doi] AB - Men occupy a privileged position within gender power relations: the male body has been the default reference point for biomedical research and therapeutic development (with the historical exclusion of women from medical trials), and men dominate health services leadership and exercise well documented medical misogyny. Yet despite their privileged position, many aspects of men's health outcomes are neglected and undertheorised because they are not examined through an intersectional gender lens. Although consideration of gender and masculinity is important for understanding men's health, research thus far has focused predominantly on men's harmful health behaviours, overemphasising individual determinants of health, and a deficit-based approach. Little attention has been paid to the influence of structural determinants of men's health risks and vulnerabilities, or the gender-specific health outcomes of worsening social and economic inequalities. In this first paper of a Lancet Series on men's health, we present a conceptual framework for understanding men's health from an intersectional perspective, highlighting how structural, social, and commercial determinants of health, intersectionality, and syndemic theory can be combined to analyse men's health at different socioecological levels. We illustrate the framework using four selected case studies that show how the health outcomes of marginalised men are influenced by complex interactions. We provide a working definition of men's health that reflects existing literature and builds on the conceptual framework and case studies. Promotion of gender justice and health equity requires moving beyond one-dimensional approaches that construct men as problematic individuals, and towards better understanding and action on the broader set of power systems and structural determinants underpinning men's health. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY 4.0 license. Published by Elsevier Ltd.. All rights reserved. FAU - Tolhurst, Rachel AU - Tolhurst R AD - Department of International Public Health, Liverpool School of Tropical Medicine, Liverpool, UK. FAU - Egid, Beatrice R AU - Egid BR AD - Department of International Public Health, Liverpool School of Tropical Medicine, Liverpool, UK. Electronic address: beatrice.egid@lstmed.ac.uk. FAU - Mavhu, Webster AU - Mavhu W AD - Centre for Sexual Health and HIV/AIDS Research (CeSHHAR), Harare, Zimbabwe. FAU - Catto, James W F AU - Catto JWF AD - Division of Clinical Medicine, School of Medicine & Population Health, University of Sheffield, Sheffield, UK. FAU - Fouad, Fouad AU - Fouad F AD - Department of International Public Health, Liverpool School of Tropical Medicine, Liverpool, UK. FAU - Griffith, Derek M AU - Griffith DM AD - Department of Family and Community Health, School of Nursing, University of Pennsylvania, Philadelphia, PA, USA. FAU - Guha-Sapir, Debarati AU - Guha-Sapir D AD - Catholic University of Louvain, Louvain-la-Neuve, Belgium. FAU - Hawkes, Sarah J AU - Hawkes SJ AD - Department of Global Population Health, School of Medicine and Health Sciences, Monash University Malaysia, Subang Jaya, Malaysia; Global 50/50, Cambridge, UK. FAU - Jahan, Ishrat AU - Jahan I AD - BRAC James P Grant School of Public Health, BRAC University, Dhaka, Bangladesh. FAU - Katikireddi, Srinivasa Vittal AU - Katikireddi SV AD - School of Health and Wellbeing, University of Glasgow, Glasgow, UK. FAU - Mwiine, Amon Ashaba AU - Mwiine AA AD - Makerere University, Kampala, Uganda. FAU - Rashid, Sabina F AU - Rashid SF AD - BRAC James P Grant School of Public Health, BRAC University, Dhaka, Bangladesh. FAU - Ringwald, Beate AU - Ringwald B AD - Department of International Public Health, Liverpool School of Tropical Medicine, Liverpool, UK. FAU - Thomson, Rachael AU - Thomson R AD - Centre for Tuberculosis Research, Liverpool School of Tropical Medicine, Liverpool, UK. FAU - Thomson, Rachel M AU - Thomson RM AD - School of Health and Wellbeing, University of Glasgow, Glasgow, UK. FAU - Ugwu, Chukwuebuka AU - Ugwu C AD - Department of International Public Health, Liverpool School of Tropical Medicine, Liverpool, UK. FAU - Theobald, Sally AU - Theobald S AD - Department of International Public Health, Liverpool School of Tropical Medicine, Liverpool, UK. LA - eng PT - Journal Article PT - Review PL - England TA - Lancet JT - Lancet (London, England) JID - 2985213R SB - IM MH - Humans MH - *Men's Health MH - Male MH - Masculinity MH - *Syndemic MH - Health Behavior COIS- Declaration of interests RTo, BRE, SFR, and ST were supported by funding from the UK Research and Innovation (UKRI) Global Challenges Research Fund Accountability for Informal Urban Equity Hub: a UKRI Collective Fund award with award reference ES/S00811X/1. ST, SFR, WM, and RTo were supported with funding from Gender Equity and Antimicrobial Resistance, funded by the Department of Health and Social Care's Fleming Fund using UK aid/MottMacDonald (grant number FF152 558). ST is a part-time UKRI-Foreign, Commonwealth & Development Office (FCDO) Senior Research Fellow in Gender (2024-2027, payment to the institution), and the views expressed in this publication are those of the authors and not necessarily those of UKRI-FCDO or any other funder. ST has received payments and travel expenses from the Wellcome Trust. RTo is supported by funding from the Canadian International Development Research Centre and UK FCDO for Urban Transformative Research for Adaptation to Climate Change in Informal Settlements (grant number 110642-001). JWFC has received a grant from Roche (to their institution); consulting fees or honoraria from Astellas, AstraZeneca, BMS, CG Oncology, Roche, Ferring, Janssen, Johnson and Johnson, Medscape, Pfizer, and Merck; support for attending meetings from Janssen; has participated on the External Advisory Boards for the PROMOTE trial (University of Oxford, UK), the Bespoke trial (University College London Hospitals, UK), and the CISTO trial (Bladder Cancer Advocacy Network, USA); has played a leadership/fiduciary role with Fight Bladder Cancer UK and Weston Park Cancer Charity; and is Director of the Sheffield National Institute for Health and Care Research (NIHR) Clinical Research Facility. DMG declares National Institute on Minority Health and Health Disparities funding for Fathers and Children Exercising Together (FACEiT) & Black Health Block & Quit and Screen Project - Supplement to University of Arkansas for Medical Sciences P50, US Department of Defense funding for Impact of Online Prostate Cancer Information on Health Disparities, Movember Foundation funding for Rooted & Rising Collective: Movember Foundation Accelerator Program for Young Black Men; payment for lecture for Men's Health World Congress, National Maternal Health Innovation Symposium, University of Minnesota's 8th Annual Research Ethics Day Conference, Community Engagement Grand Rounds (University of Texas Southwestern Medical Center), 13th Annual Health Equity & Social Justice Conference, Annual Public Health Conference Promoting Health Equity, Speaker Series University of Arkansas for Medical Sciences (Center for Research, Health and Social Justice), Promoting Father Engagement to Enhance Baby's Brain Health Development (School of Public Health, University of Texas), Equity ECHO Series-The Ethics of Doing Research with Communities (University of New Mexico Health Science Center), Partners for Advancing Health Equity (P4HE) webinar (Tulane School of Public Health and Tropical Medicine), Curtis Center Health Equity Seminar at University of Michigan, Black Health & Wellness Speaker Series at University of Pittsburgh, Health Equity: From Lens to Science series at University of California, Workshop at Rutgers University, Precision Prevention, Early Detection, and Interception of Cancer Special Conference at American Association for Cancer Research; member of Global Men's Health Advisory Committee (Movember Foundation) and Men's Health Network, Chair of Global Action on Men's Health. SJH declares grants to Global 50/50 from the Gates Foundation and Co-Impact; consulting fees from Elsevier Research Cultures Board and the Swedish Government Research Funding Board; payment or honoraria from the Swiss National Science Foundation (paid to Global 50/50); has participated on an advisory board with WHO as chair of Data Safety and Monitoring Board trial; and is a UK Government-appointed Commissioner and Chair of the Awards Policy Committee for the Commonwealth Scholarship Commission. SVK was supported by funding from the European Research Council (949582), the Medical Research Council (MRC; MC_UU_00022/2), and the Scottish Government Chief Scientist Office (SPHSU17); has received reimbursement from the MRC to attend grant panel meetings; and is an unpaid member of Obesity Action Scotland. BR, RTh, and CU were supported by funding from UK aid Leaving No One Behind; transforming Gendered pathways to Health for TB Research Programme Consortium. RMT received a Wellcome Trust Research Award for Health Professionals (218105/Z/19/Z), the NIHR Development Award (co-primary investigator; NIHR154243), the NIHR Researcher-Led Award (co-investigator; NIHR168008), and a Public Health Scotland award (Safer Drugs Consumption Facility Evaluation - Baseline Data Collection). All other authors declare no competing interests. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 19:52 PHST- 2025/03/18 00:00 [received] PHST- 2026/06/24 00:00 [revised] PHST- 2026/07/20 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:52 [entrez] AID - S0140-6736(26)01489-3 [pii] AID - 10.1016/S0140-6736(26)01489-3 [doi] PST - ppublish SO - Lancet. 2026 Oct 31;408(10566):1866-1880. doi: 10.1016/S0140-6736(26)01489-3. PMID- 42956772 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1474-547X (Electronic) IS - 0140-6736 (Linking) VI - 408 IP - 10566 DP - 2026 Oct 31 TI - Non-invasive tests in metabolic dysfunction-associated steatotic liver disease: the LITMUS prospective multicentre diagnostic accuracy study. PG - 1850-1865 LID - S0140-6736(26)01145-1 [pii] LID - 10.1016/S0140-6736(26)01145-1 [doi] AB - BACKGROUND: The advent of regulatory-approved pharmacological treatments for metabolic dysfunction-associated steatotic liver disease (MASLD) calls for precise, non-invasive biomarkers that facilitate early diagnosis, support therapy selection, and aid clinical trial enrolment. The European multicentre LITMUS study aimed to evaluate the diagnostic performance of a broad range of non-invasive biomarkers for identifying patients with metabolic dysfunction-associated steatohepatitis (MASH), at-risk MASH, advanced fibrosis, and cirrhosis. METHODS: In this prospective, multicentre, diagnostic accuracy study, adults with biopsy-confirmed MASLD were prospectively recruited across 13 countries in Europe and the USA. We assessed 12 single markers and 18 multimarker panels. The reference standard was centrally scored liver histology according to the non-alcoholic steatohepatitis Clinical Research Network Scoring System. Target conditions included MASH (Non-Alcoholic Fatty Liver Disease Activity Score >/=4 with >/=1 point per component), at-risk MASH (MASH plus significant fibrosis [fibrosis stage F>/=2]), advanced fibrosis (F>/=3), and cirrhosis (F4). Performance was evaluated by area-under-the-receiver operating characteristic curve (AUC), with the minimum acceptable criterion (MAC) of AUC higher than 0.80 to indicate potential biopsy replacement. FINDINGS: Between Jan 1, 2018, and June 30, 2023, we analysed data from 1590 participants with centrally scored liver biopsies and at least one biomarker measurement; 609 (38%) had biopsy-confirmed at-risk MASH, 678 (43%) had MASH, 585 (37%) had advanced fibrosis, and 164 (10%) had cirrhosis. For detecting at-risk MASH, SomaSignal showed the highest diagnostic performance (AUC 0.83 [95% CI 0.80-0.85]), significantly exceeding MAC, whereas no other test reached the MAC for MASH only. For advanced fibrosis, SomaSignal showed the highest performance (AUC 0.92 [0.90-0.94]), followed by FibroMeter-VCTE (AUC 0.87 [0.83-0.92]), Agile 3+ and TSP2 (both AUC 0.84 [0.82-0.86]), and NIS4 (0.82 [0.80-0.85]). For cirrhosis, FibroMeter-VCTE showed the highest diagnostic performance (AUC 0.94 [0.90-0.97]) followed by SomaSignal (AUC 0.92 [0.90-0.94]), followed by Agile 3+ (0.88 [0.85-0.91]), Agile 4 (0.87 [0.84-0.90]), ADAPT (0.85 [0.81-0.88]), and ELF, LSM-VCTE, and TSP2 (all 0.84 [0.81-0.87]). Sequential two-step approaches using gamma-glutamyl transferase as a first-line test met the MAC for both advanced fibrosis and at-risk MASH. INTERPRETATION: Although single markers resulted in variable and limited performance when compared to histology for detecting at-risk MASH, multimarker panels showed great potential, with a proteomic panel showing strong diagnostic performance. These findings could support the selection and implementation of non-invasive testing strategies in MASLD and inform future clinical guidelines, regulatory evaluation, and clinical trial design. FUNDING: Innovative Medicines Initiative 2 Joint Undertaking. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved, including those for text and data mining, AI training, and similar technologies. FAU - Vali, Yasaman AU - Vali Y AD - Department of Epidemiology and Data Science, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, Netherlands. Electronic address: y.vali@amsterdamumc.nl. FAU - Wonders, Kristy AU - Wonders K AD - Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University, Newcastle-upon-Tyne, UK. FAU - Lee, Jenny AU - Lee J AD - Department of Epidemiology and Data Science, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, Netherlands. FAU - Aithal, Guruprasad P AU - Aithal GP AD - NIHR Nottingham Biomedical Research Centre, Nottingham University Hospitals NHS Trust and University of Nottingham, Nottingham, UK. FAU - Aller, Rocio AU - Aller R AD - Department of Medicine, Dermatology, and Toxicology, Universidad de Valladolid, Madrid, Spain; Miembro UIC Biocritic, CIBERINFEC, Madrid, Spain. FAU - Allison, Michael E D AU - Allison MED AD - Cambridge Liver Unit, Cambridge NIHR Biomedical Research Centre, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK. FAU - Arola, Johanna AU - Arola J AD - Department of Pathology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. FAU - Banales, Jesus M AU - Banales JM AD - Department of Liver and Gastrointestinal Diseases, Biogipuzkoa Health Research Institute, Donostia University Hospital, University of the Basque Country (UPV/EHU), Ikerbasque, CIBERehd, San Sebastian, Spain; Department of Biochemistry and Genetics, School of Sciences, University of Navarra, Pamplona, Spain. FAU - Bernal-Monterde, Vanesa AU - Bernal-Monterde V AD - Department of Gastroenterology and Hepatology, Miguel Servet University Hospital, IISA, Zaragoza, Spain. FAU - Berzigotti, Annalisa AU - Berzigotti A AD - Department of Visceral Surgery and Medicine, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland. FAU - Boursier, Jerome AU - Boursier J AD - Service d'Hepato-Gastroenterologie, Centre Hospitalier Universitaire d'Angers, Angers, France; Laboratoire HIFIH, SFR ICAT 4208, Universite d'Angers, Angers, France. FAU - Brass, Clifford A AU - Brass CA AD - Resolution Therapeutics, East Hampton, NY, USA. FAU - Bugianesi, Elisabetta AU - Bugianesi E AD - Department of Medical Sciences, Division of Gastrohepatology, City of Health and Science of Turin, University of Turin, Turin, Italy. FAU - Calleja, Jose Luis AU - Calleja JL AD - Department of Gastroenterology and Hepatology, Hospital Universitario Puerta de Hierro, Universidad Autonoma de Madrid, Madrid, Spain. FAU - Cobbold, Jeremy F AU - Cobbold JF AD - Translational Gastroenterology and Liver Unit, University of Oxford, Oxford, UK; Oxford NIHR Biomedical Research Centre, Oxford University Hospitals NHS Foundation Trust and the University of Oxford, Oxford, UK. FAU - Cortez-Pinto, Helena AU - Cortez-Pinto H AD - Clinica Universitaria de Gastrenterologia, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal. FAU - Crespo, Javier AU - Crespo J AD - Gastroenterology and Hepatology Department, Clinical and Translational Research in Digestive Diseases, Valdecilla Research Institute (IDIVAL), Marques de Valdecilla University Hospital, Santander, Spain. FAU - Davies, Susan AU - Davies S AD - Cambridge Liver Unit, Cambridge NIHR Biomedical Research Centre, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK. FAU - Driessen, Ann AU - Driessen A AD - Department of Gastroenterology Hepatology, Antwerp University Hospital, Antwerp, Belgium; Translational Sciences in Inflammation and Immunology, InflaMed Centre of Excellence, Laboratory of Experimental Medicine and Paediatrics, Faculty of Medicine and Health Sciences, University of Antwerp, Antwerp, Belgium. FAU - Duffin, Kevin L AU - Duffin KL AD - Lilly Research Laboratories, Eli Lilly and Company, Lilly Corporate Center, Indianapolis, IN, USA. FAU - Ekstedt, Mattias AU - Ekstedt M AD - Department of Health, Medicine, and Caring Sciences, Linkoping University, Linkoping, Sweden. FAU - Forton, Daniel AU - Forton D AD - NIHR St Georges Clinical Research Facility, St Georges Hospital, London, UK. FAU - Fournier-Poizat, Celine AU - Fournier-Poizat C AD - Echosens, Paris, France. FAU - Francque, Sven AU - Francque S AD - Department of Gastroenterology Hepatology, Antwerp University Hospital, Antwerp, Belgium. FAU - Geier, Andreas AU - Geier A AD - Department of Hepatology, University Hospital Wurzburg, Wurzburg, Germany. FAU - Gogain, Joseph AU - Gogain J AD - SomaLogic, Boulder, CO, USA. FAU - Gouw, Annette S H AU - Gouw ASH AD - Department of Pathology and Medical Biology, University Medical Centre Groningen, Groningen, Netherlands. FAU - Hagstrom, Hannes AU - Hagstrom H AD - Division of Hepatology, Department of Upper Gastrointestinal Diseases, Karolinska University Hospital, Stockholm, Sweden. FAU - Holleboom, Adriaan G AU - Holleboom AG AD - Department of Internal and Vascular Medicine, Amsterdam University Medical Centres, University of Amsterdam, Amsterdam, Netherlands. FAU - Hytiroglou, Prodromos AU - Hytiroglou P AD - Department of Pathology, School of Medicine, Aristotle University, Thessaloniki, Greece. FAU - Karsdal, Morten AU - Karsdal M AD - Nordic Bioscience, Biomarkers, and Research, Herlev, Denmark. FAU - Lackner, Carolin AU - Lackner C AD - Institute of Pathology, Medical University of Graz, Graz, Austria. FAU - Leeming, Diana J AU - Leeming DJ AD - Nordic Bioscience, Biomarkers, and Research, Herlev, Denmark. FAU - Miele, Luca AU - Miele L AD - Department of Translational Medicine and Surgery, Universita Cattolica del Sacro Cuore and Fondazione Policlinico Gemelli IRCCS, Rome, Italy. FAU - Papatheodoridis, George AU - Papatheodoridis G AD - Department of Gastroenterology Laiko General Hospital, Medical School of National and Kapodistrian University of Athens, Athens, Greece. FAU - Paradis, Valerie AU - Paradis V AD - Pathology Department, Beaujon Hospital, APHP, Inserm UMR1149, Paris, France. FAU - Pavlides, Michael AU - Pavlides M AD - Translational Gastroenterology and Liver Unit, University of Oxford, Oxford, UK; Oxford NIHR Biomedical Research Centre, Oxford University Hospitals NHS Foundation Trust and the University of Oxford, Oxford, UK. FAU - Pericas, Juan M AU - Pericas JM AD - Liver Unit, Vall d'Hebron University Hospital, Vall d'Hebron Institute for Research, Universitat Autonoma de Barcelona, Spanish Network of Biomedical Research Centres in Hepatic and Digestive Diseases (CIBERehd), Barcelona, Spain. FAU - Petta, Salvatore AU - Petta S AD - Sezione di Gastroenterologia, Dipartimento Promozione della Salute, Materno-Infantile, di Medicina Interna e Specialistica di Eccellenza G D'Alessandro, Universita di Palermo, Palermo, Italy. FAU - Ratziu, Vlad AU - Ratziu V AD - Assistance Publique-Hopitaux de Paris, Hopital Beaujon, University Paris-Diderot, Paris, France. FAU - Romero-Gomez, Manuel AU - Romero-Gomez M AD - UCM Digestive Diseases, Virgen del Rocio University Hospital, Instituto de Biomedicina de Sevilla, CIBEREHD, University of Seville, Sevilla, Spain. FAU - Schattenberg, Jorn M AU - Schattenberg JM AD - Department of Medicine II, University Medical Center Homburg, Homburg, Germany. FAU - Schuppan, Detlef AU - Schuppan D AD - Institute of Translational Immunology, University Medical Centre, Mainz, Germany. FAU - Surabattula, Rambabu AU - Surabattula R AD - Institute of Translational Immunology, University Medical Centre, Mainz, Germany. FAU - Silva, Ana Paula AU - Silva AP AD - Unidade Local de Saude Gaia Espinho, Rua Conceicao Fernandes, Vila Nova de Gaia, Portugal. FAU - Kjaer, Mette Skalshoi AU - Kjaer MS AD - Novo Nordisk, Soborg, Denmark. FAU - Straub, Beate K AU - Straub BK AD - Institute of Translational Immunology, University Medical Centre, Mainz, Germany. FAU - Svegliati-Baroni, Gianluca AU - Svegliati-Baroni G AD - Liver Injury and Transplant Unit, Polytechnic University of Marche, Via Conca, Ancona, Italy. FAU - Tiniakos, Dina AU - Tiniakos D AD - Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University, Newcastle-upon-Tyne, UK; Department of Pathology, Aretaieion Hospital, Medical School of National and Kapodistrian University of Athens, Athens, Greece. FAU - Tonini, Maria Manuela AU - Tonini MM AD - Luxembourg Institute of Health, Translational Medicine Operations Hub, Dudelange, Luxembourg. FAU - Torstenson, Richard AU - Torstenson R AD - Astrazeneca, Regulatory Affairs, Cardiovascular, Renal, and Metabolism (CVRM), BioPharmaceuticals R&D, Gothenburg, Sweden. FAU - Valenti, Luca V C AU - Valenti LVC AD - Department of Pathophysiology and Transplantation, Universita degli Studi di Milano, Milan, Italy; Precision Medicine and Biological Resource Centre, Fondazione IRCCS Ca'Granda Ospedale Maggiore Policlinico, Milan, Italy. FAU - Verheij, Joanne AU - Verheij J AD - Department of Hepatopancreatobiliary Pathology, Amsterdam University Medical Centers, Amsterdam, Netherlands. FAU - Magnanensi, Jeremy AU - Magnanensi J AD - Genfit, Loos, France. FAU - Mayo, Rebeca AU - Mayo R AD - Rubio Metabolomics, Parque Tecnologico de Bizkaia, Derio, Spain. FAU - Wenn, David AU - Wenn D AD - iXscient, Twickenham, UK. FAU - Yki-Jarvinen, Hannele AU - Yki-Jarvinen H AD - Department of Medicine, University of Helsinki and Minerva Foundation Institute for Medical Research, Helsinki, Finland. FAU - Yunis, Carla AU - Yunis C AD - Pfizer Research and Development, New York, NY, USA. FAU - Bossuyt, Patrick M AU - Bossuyt PM AD - Department of Epidemiology and Data Science, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, Netherlands. FAU - Anstee, Quentin M AU - Anstee QM AD - Translational and Clinical Research Institute, Faculty of Medical Sciences, Newcastle University, Newcastle-upon-Tyne, UK; Newcastle NIHR Biomedical Research Centre, Newcastle-upon-Tyne Hospitals NHS Foundation Trust, Newcastle upon Tyne, UK. Electronic address: quentin.anstee@newcastle.ac.uk. CN - LITMUS Investigators LA - eng PT - Journal Article PT - Multicenter Study PL - England TA - Lancet JT - Lancet (London, England) JID - 2985213R RN - 0 (Biomarkers) SB - IM MH - Humans MH - Prospective Studies MH - Female MH - Male MH - Biomarkers/blood MH - Middle Aged MH - *Non-alcoholic Fatty Liver Disease/diagnosis MH - Liver Cirrhosis/diagnosis MH - Adult MH - Biopsy MH - Liver/pathology MH - ROC Curve MH - Europe COIS- Declaration of interests GPA received investigator initiated Grant funding from Ipsen and Pfizer, consulting fees paid to the University of Nottingham for the work unrelated to this topic from Agios Pharmaceuticals, Amryt Pharmaceuticals (from SDN486 Aegerion), AstraZeneca, BenevolventAI Bio, Clinipace, DNDi, GSK research and development (R&D), Janssen R&D, Merck Healthcare, Novartis Pharma, Pure Tech, Suzhou MDCE, and SynOx Therapeutics, UK. MEDA had research collaborations with GSK and Astra Zeneca. AB was a consultant for Boehringer-Ingelheim, advisory was on the advisory board for Astellas, and received lectures fees from GE Healthcare and Hologic. All the honoraries have been paid to MA's institution. JMB received grants and contracts from Albireo-Ipsen, Incyte, Cymabay-Gilead, Taiho, and Jazz, was a consultant for Albireo-Ipsen, CIMABay, OWL-Rubio Metabolomics, Jazz, AstraZeneca, and Servier, and a speaker for Incyte, AstraZeneca, Eisai, Advanz, Ipsen, and Servier. LM received the following grants: EP PerMed JTC2024 (grant agreement number 101137129); EU HORIZON-JU-IHI-2022-03-01 (101132946); EU HORIZON 2020 JTI-IMI2-2016-09 (777377), Italian Ministry of Health (MOH), PNRR M6/C2 2023 (PNRR-MCNT2-2023-12378247); Italian Ministry of University and Research, PRIN 2022 (2022C7ZR3W); Italian MOH, PNRR 2022 (PNRR-MAD-2022-12375633); Italian MOH, Ricerca Finalizzata 2021 (RF-2021-12372565); and was also a consultant for Alexion, AstraZeneca, Boehringer-Ingelheim, Echosens, Eli Lilly, GSK, Madrigal, Mirum Pharma, Novo-Nordisk, Orphalan, Resalis Pharma, and Siemens Healthineers, received travel support from Novo-Nordisk and Orphalan, received payments from Madrigal, MSD, Novo-Nordisk, and Echosens, and was on advisory boards for Madrigal, Boehringer-Ingelheim, and Novo-Nordisk, and holds a leadership position within the United European Gastroenterology Public Affair Group. EB received consulting or speaking fees from Boehringer Ingelheim, Eli Lilly, Madrigal, MSD, and Novo Nordisk. JFC received consulting fees from Madrigal and NovoNordisk, a speaker for Madrigal, and served as the MASLD SIG Lead within BASL (British Association for the Study of the Liver). KLD is an employee and a company stock shareholder for Eli Lilly. HC-P received personal payments and honoraria from Novo Nordisk, Advanz Pharm, Boehringer Ingelheim and Merck Sharp & Dohme. CF-P is full-time employee of Echosens. SF received the following grants and contracts: senior clinical investigator fellowship (Research Foundation Flanders 1802154N); research grants from Astellas, Falk Pharma, Genfit, Gilead Sciences, GlympsBio, Janssens Pharmaceutica, Inventiva, Merck Sharp & Dohme, Pfizer, and Roche; doctoral grants from the University of Antwerp (44430); research grants from the Belgian Association for the Study of the Liver; was Senior Clinical Investigator and received a fellowship for Research Foundation Flanders (1802154N); received royalties or licenses from the Flemish Institute for Biotechnology Grand Challenges Programme, University of Antwerp (GOA 2018 ID 36572); and consulting fees from Abbvie, Actelion, Aelin Therapeutics, AgomAb, Aligos Therapeutics, Allergan, Alnylam, Astellas, AstraZeneca, Bayer, Boehringer Ingelheim, Bristol-Meyers Squibb, CSL Behring, Coherus, Echosens, Dr Falk Pharma, Eisai, Enyo, Galapagos, Galmed, Genetech, Genfit, Genflow Biosciences, Gilead Sciences, Intercept, Inventiva, Janssens, Pharmaceutica, Pro Med CS Praha, Julius Clinical, Madrigal, Medimmune, Merck Sharp & Dohme, Mursla, NGM Bio, Novartis, Novo Nordisk, Promethera, Roche, Siemens Healthineers, Weatherden. Lecturer fees: Abbvie, Allergan, Bayer, Eisai, Genfit, Gilead Lecturer fees, Sciences, Janssens Cilag, Intercept, Inventiva, Merck Sharp & Dohme, Novo Nordisk, Promethera, and Siemens; and received support for attending meetings (travel grant NAFLD summit 2022). AG reports support from the IMI2 LITMUS project; research grants from Boehringer Ingelheim, Falk, and Intercept; consulting and speaker fees from AbbVie, Advanz, Albireo, Alexion, AstraZeneca, Bayer, Bristol Myers Squibb (BMS), Boehringer Ingelheim, Eisai, Gilead, Heel, Intercept, Ipsen, Madrigal, Merz, MSD, Novartis, Novo Nordisk, Pfizer, Roche, and Sanofi-Aventis; honoraria from AbbVie, Advanz, Alexion, BMS, Burgerstein, CSL Behring, Falk, Gilead, Intercept, Madrigal, Merz, MSD, Novartis, Novo Nordisk, Orphalan, and Roche; travel and meeting support from Intercept, Ipsen, Gilead, AbbVie, and Falk; and participation on the advisory board of Falk. CL has received consulting fees from Galmed, Falk Pharma, Proxymo, Novo Nordisk, Regeneron, Inventiva, Bio89, Akero, Boehringer Ingelheim, BMS, and Kowa, and has participated on the Data Safety Monitoring Board or Advisory Board for Kowa. JG is a former employee of SomaLogic and Standard BioTools, is currently employed by SomaLogic and Illumina, and holds stock in Standard BioTools. PH reports consultancy fees from ICON Research Ltd and Inventiva SA. MK is Chief Executive Officer of Nordic Bioscience and holds stock in the company. DJL holds stock options in Nordic Bioscience. GP has received research grants from AbbVie, Gilead, and Vianex; consulting fees from AbbVie, Amgen, AstraZeneca, BioArs, Gilead, GlaxoSmithKline, Ipsen, Janssen, Novo Nordisk, Roche, Takeda, and Vir Biotechnology; and has served as an advisor or lecturer for AbbVie, AstraZeneca, Gilead, Ipsen, Novo Nordisk, and Roche. MP reports funding from the Innovative Medicines Initiative 2 Joint Undertaking (777377), a grant from the Oxford NIHR Biomedical Research Centre, and is a shareholder in Perspectum. JMP reports consulting fees from Novo Nordisk, Madrigal, and Boehringer Ingelheim; honoraria for lectures and presentations from Novo Nordisk, Madrigal, Boehringer Ingelheim, Siemens, and Novartis; and travel support from Boehringer Ingelheim, Madrigal, and Novo Nordisk. VR reports consulting fees from HistoIndex, PharmaNest, PathAI, Akero, 89Bio, Novo Nordisk, Boehringer Ingelheim, GSK, and Sagimet. MR-G reports research grants from Siemens Healthineers, Gilead, Echosens, Intercept, and Novo Nordisk; consulting fees from Boehringer Ingelheim, Alfasigma, MSD, Gilead, UCB Pharma, Rubio, Ipsen, Siemens, Novo Nordisk, Shionogi, and Sobi; and participation on the advisory board of Galmed. JMS reports consultancy fees from Akero, Alexion, Altimmune, AstraZeneca, 89Bio, Boehringer Ingelheim, Gilead Sciences, GSK, Ipsen, Inventiva Pharma, Madrigal Pharmaceuticals, Merck, Kriya Therapeutics, Echosens, Eli Lilly, E-Therapeutics, MSD Sharp & Dohme, Novo Nordisk, Roche, and Vantage Biosciences; honoraria from AbbVie, Boehringer Ingelheim, Echosens, Gilead Sciences, Medscape, Novo Nordisk, and Madrigal Pharmaceuticals; and stock options in Hepta Bio. MSK is a full-time employee and shareholder of Novo Nordisk, which also provides travel and meeting support. DT reports consulting fees (paid to the University of Athens) from Inventiva, ICON, Clinnovate, and CymaBay; honoraria for lectures and presentations from the Belgian Society of Gastroenterology and the August Pi i Sunyer Biomedical Research Institute, Barcelona, Spain; serves as Chair of the Advisory Board of the European Society of Pathology; and is General Partner at HEXAGON Pathology Consulting Services. JM is an employee of Genfit and holds stock in Genfit. RM is an employee of Rubio Metabolomics. HY-J reports consultancy fees from Novartis, Novo Nordisk, Eli Lilly, and MSD, and has served on advisory boards for MSD, Eli Lilly, and Hamni. DS reports funding from the EU IMI LITMUS project (2020-2025) for biomarker development and validation; consultancy fees from Prosciento and Resalis; service on a Data Monitoring Committee for Boehringer Ingelheim; and stock options in NorthSea Therapeutics and Resalis. JB reports research grants and speaker fees from Echosens and Siemens and has royalties/licenses from Echosens. LVCV reports consulting and speaker fees from Viatris, Novo Nordisk, and GSK; consulting fees from Novo Nordisk, Pfizer, Boehringer Ingelheim, Resalis, GSK, Almac, AIRNA, AstraZeneca, and Echosens; and travel support from Novo Nordisk. ME reports institutional research grant funding from Takeda and AstraZeneca; speaker fees from Novo Nordisk, Mediplast, and All About Meetings; and personal honoraria from Ipsen. BKS reports a research grant from German Cancer Aid; consulting fees from Bristol Myers Squibb; speaker fees from Boehringer Ingelheim, MedUpdate, and IMPP; writing fees from Springer Nature; and travel support from AstraZeneca. JLC reports research funding from Gilead; consulting fees from Gilead Sciences, Echosens, Novo Nordisk, and Madrigal; honoraria for lectures from Gilead, AbbVie, Roche, Madrigal, Novo Nordisk, AstraZeneca, and MSD; and travel support from AstraZeneca, AbbVie, Roche, and Gilead. CY is an employee and shareholder of Pfizer and is also a shareholder in Eli Lilly, Inventiva, and Amgen. QMA reports research grant funding from AstraZeneca, Boehringer Ingelheim, and Intercept; consultancy (on behalf of Newcastle University) for AbbVie, Alimentiv, Akero, AstraZeneca, Axcella, 89Bio, Boehringer Ingelheim, Boston Pharmaceuticals, Corcept Therapeutics, Echosens, Eli Lilly, Gilead, GlaxoSmithKline, HistoIndex, Intercept, Inventiva, Madrigal, Medpace, Merck, Metadeq, Novo Nordisk, PathAI, Pfizer, PharmaNest, Prosciento, Regeneron, Resolution Therapeutics, Roche, and Terns; speaker fees from Avalere, Catalyst Medical Education, Integritas Communications, Medscape, Novo Nordisk, and Springer Healthcare; and advisory board participation (on behalf of Newcastle University) for Medpace and Gilead. DF reports honoraria from Madrigal and Gilead; expert testimony for the UK National Audit Office; and participation on the advisory board of Resolution Therapeutics. HH reports institutional research funding from AstraZeneca, MSD, Novo Nordisk, and Takeda; consulting fees from AstraZeneca, Novo Nordisk, GSK, and Quality Refresh; honoraria for lectures from Boehringer Ingelheim, Novo Nordisk, and Ipsen; and participation in hepatic events adjudication committees for Arrowhead, GSK, Kowa, Jazz Pharmaceuticals, and Boehringer Ingelheim. JV reports payments for work undertaken for Takeda (2025). CAB reports consulting fees from Resolution Therapeutics and holds stock in Novartis and Merck (from previous employment) and Resolution Therapeutics (current employment). AD reports honoraria for lectures and presentations from BeOne Medicines (formerly BeiGene), paid to her institution. RT holds stock in AstraZeneca. All other authors declare no competing interests. FIR - Anstee, Quentin M IR - Anstee QM FIR - Daly, Ann IR - Daly A FIR - Oakley, Fiona IR - Oakley F FIR - Cockell, Simon J IR - Cockell SJ FIR - Tiniakos, Dina IR - Tiniakos D FIR - Bedossa, Pierre IR - Bedossa P FIR - Cordell, Heather J IR - Cordell HJ FIR - Day, Christopher P IR - Day CP FIR - Wonders, Kristy IR - Wonders K FIR - Govaere, Olivier IR - Govaere O FIR - Missier, Paolo IR - Missier P FIR - McTeer, Matthew IR - McTeer M FIR - Vale, Luke IR - Vale L FIR - Oliboyede, Yemi IR - Oliboyede Y FIR - Breckons, Matt IR - Breckons M FIR - Bossuyt, Patrick M IR - Bossuyt PM FIR - Vali, Yasaman IR - Vali Y FIR - Lee, Jenny IR - Lee J FIR - Nieuwdorp, Max IR - Nieuwdorp M FIR - Acherman, Yair IR - Acherman Y FIR - van de Laar, Arnold IR - van de Laar A FIR - Angelakis, Athanasios IR - Angelakis A FIR - Holleboom, Adriaan G IR - Holleboom AG FIR - Verheij, Joanne IR - Verheij J FIR - Ratziu, Vlad IR - Ratziu V FIR - Clement, Karine IR - Clement K FIR - Patino-Navarrete, Rafael IR - Patino-Navarrete R FIR - Pais, Raluca IR - Pais R FIR - Paradis, Valerie IR - Paradis V FIR - Wagner, Mathilde IR - Wagner M FIR - Schattenberg, Jorn M IR - Schattenberg JM FIR - Michel, Maurice IR - Michel M FIR - Schuppan, Detlef IR - Schuppan D FIR - Myneni, Sudha IR - Myneni S FIR - Surabattula, Rambabu IR - Surabattula R FIR - Straub, Beate K IR - Straub BK FIR - Emrich, Tilman IR - Emrich T FIR - Vidal-Puig, Toni IR - Vidal-Puig T FIR - Vacca, Michele IR - Vacca M FIR - Rodrigues-Cuenca, Sergio IR - Rodrigues-Cuenca S FIR - Allison, Michael Ed IR - Allison ME FIR - Kamzolas, Ioannis IR - Kamzolas I FIR - Petsalaki, Evangelia IR - Petsalaki E FIR - Campbell, Mark IR - Campbell M FIR - Lelliott, Chris J IR - Lelliott CJ FIR - Davies, Susan IR - Davies S FIR - Godfrey, Edmund IR - Godfrey E FIR - Oresic, Matej IR - Oresic M FIR - Hyotylainen, Tuulia IR - Hyotylainen T FIR - McGlinchey, Aidan IR - McGlinchey A FIR - Mato, Jose M IR - Mato JM FIR - Millet, Oscar IR - Millet O FIR - Berzigotti, Annalisa IR - Berzigotti A FIR - Dufour, Jean-Francois IR - Dufour JF FIR - Lange, Naomi F IR - Lange NF FIR - Huber, Adrian T IR - Huber AT FIR - Pavlides, Michael IR - Pavlides M FIR - Cobbold, Jeremy F IR - Cobbold JF FIR - Mozes, Ferenc E IR - Mozes FE FIR - Akhtar, Salma IR - Akhtar S FIR - Harrison, Stephen A IR - Harrison SA FIR - Olodo-Atitebi, Seliat IR - Olodo-Atitebi S FIR - Banerjee, Rajarshi IR - Banerjee R FIR - Shumbayawonda, Elizabeth IR - Shumbayawonda E FIR - Dennis, Andrea IR - Dennis A FIR - Andersson, Anneli IR - Andersson A FIR - Romero-Gomez, Manuel IR - Romero-Gomez M FIR - Gallego-Duran, Rocio IR - Gallego-Duran R FIR - Montero-Vallejo, Rocio IR - Montero-Vallejo R FIR - Fernandez-Lizarazu, Isabel IR - Fernandez-Lizarazu I FIR - Gato, Sheila IR - Gato S FIR - Castell, Javier IR - Castell J FIR - Karsdal, Morten IR - Karsdal M FIR - Leeming, Diana Julie IR - Leeming DJ FIR - Musa, Kishwar IR - Musa K FIR - Tonini, Maria Manuela IR - Tonini MM FIR - Bugianesi, Elisabetta IR - Bugianesi E FIR - Rosso, Chiara IR - Rosso C FIR - Armandi, Angelo IR - Armandi A FIR - Faletti, Ricardo IR - Faletti R FIR - Marra, Fabio IR - Marra F FIR - Gastaldelli, Amalia IR - Gastaldelli A FIR - Carli, Fabrizia IR - Carli F FIR - Svegliati-Baroni, Gianluca IR - Svegliati-Baroni G FIR - Boursier, Jerome IR - Boursier J FIR - de Saint Loup, Marc IR - de Saint Loup M FIR - Celea, Pierre IR - Celea P FIR - Aube, Christophe IR - Aube C FIR - Francque, Sven Ma IR - Francque SM FIR - Kwanten, Wilhelmus J IR - Kwanten WJ FIR - Vonghia, Luisa IR - Vonghia L FIR - Verrijken, An IR - Verrijken A FIR - Dirinck, Eveline IR - Dirinck E FIR - Driessen, Ann IR - Driessen A FIR - Ekstedt, Mattias IR - Ekstedt M FIR - Kechagias, Stergios IR - Kechagias S FIR - Lundberg, Peter IR - Lundberg P FIR - Yki-Jarvinen, Hannele IR - Yki-Jarvinen H FIR - Porthan, Kimmo IR - Porthan K FIR - Arola, Johanna IR - Arola J FIR - van Mil, Saskia IR - van Mil S FIR - Papatheodoridis, George IR - Papatheodoridis G FIR - Cortez-Pinto, Helena IR - Cortez-Pinto H FIR - Machado, Mariana Verdelho IR - Machado MV FIR - Carvalhana, Sofia IR - Carvalhana S FIR - Rodrigues, Cecilia M P IR - Rodrigues CMP FIR - Valenti, Luca Vc IR - Valenti LV FIR - Pelusi, Serena IR - Pelusi S FIR - Petta, Salvatore IR - Petta S FIR - Penisi, Grazia IR - Penisi G FIR - Pipitone, Rosaria Maria IR - Pipitone RM FIR - Tulone, Adele IR - Tulone A FIR - Grimaudo, Stefania IR - Grimaudo S FIR - Antonucci, Michela IR - Antonucci M FIR - Miele, Luca IR - Miele L FIR - Liguori, Antonio IR - Liguori A FIR - D'Ambrosio, Francesca IR - D'Ambrosio F FIR - Geier, Andreas IR - Geier A FIR - Rau, Monika IR - Rau M FIR - Leicht, Hans Benno IR - Leicht HB FIR - Reiter, Florian IR - Reiter F FIR - Aithal, Guruprasad P IR - Aithal GP FIR - Francis, Susan IR - Francis S FIR - Palaniyappan, Naaventhan IR - Palaniyappan N FIR - Bradley, Christopher IR - Bradley C FIR - Hockings, Paul D IR - Hockings PD FIR - Schneider, Moritz J IR - Schneider MJ FIR - Newsome, Philip N IR - Newsome PN FIR - Wenn, David IR - Wenn D FIR - Magnanensi, Jeremy IR - Magnanensi J FIR - Trylesinski, Aldo IR - Trylesinski A FIR - Mayo, Rebeca IR - Mayo R FIR - Alonso, Cristina IR - Alonso C FIR - Duffin, Kevin L IR - Duffin KL FIR - Perfield, James W IR - Perfield JW FIR - Hartman, Mark L IR - Hartman ML FIR - Chen, Yu IR - Chen Y FIR - Yunis, Carla IR - Yunis C FIR - Tuthill, Theresa IR - Tuthill T FIR - Ross, Trenton IR - Ross T FIR - Bernardo, Barbara IR - Bernardo B FIR - Harrington, Magdalena Alicja IR - Harrington MA FIR - McLeod, Euan IR - McLeod E FIR - Ertle, Judith IR - Ertle J FIR - Younes, Ramy IR - Younes R FIR - Coxson, Harvey IR - Coxson H FIR - Gogain, Joseph IR - Gogain J FIR - Biegel, Hannah R IR - Biegel HR FIR - Alexander, Leigh IR - Alexander L FIR - Ostroff, Rachel IR - Ostroff R FIR - Kjaer, Mette Skalshoi IR - Kjaer MS FIR - Harder, Lea Morch IR - Harder LM FIR - Veidal, Sanne S IR - Veidal SS FIR - Al-Sari, Naba IR - Al-Sari N FIR - Kring Rasmussen, Daniel Guldager IR - Kring Rasmussen DG FIR - Balp, Maria-Magdalena IR - Balp MM FIR - Brass, Clifford A IR - Brass CA FIR - Jennings, Lori IR - Jennings L FIR - Martic, Miljen IR - Martic M FIR - Loffler, Jurgen IR - Loffler J FIR - Applegate, Douglas IR - Applegate D FIR - Torstenson, Richard IR - Torstenson R FIR - Linden, Daniel IR - Linden D FIR - Fournier-Poizat, Celine IR - Fournier-Poizat C FIR - Llorca, Anne IR - Llorca A FIR - Kalutkiewicz, Michael IR - Kalutkiewicz M FIR - Ehman, Richard L IR - Ehman RL FIR - Pepin, Kay IR - Pepin K FIR - Horan, Gerald IR - Horan G FIR - Ho, Gideon IR - Ho G FIR - Tai, Dean IR - Tai D FIR - Chng, Elaine IR - Chng E FIR - Ren, Yayun IR - Ren Y FIR - Patterson, Scott D IR - Patterson SD FIR - Billin, Andrew N IR - Billin AN FIR - Doward, Lynda C IR - Doward LC FIR - Twiss, James IR - Twiss J FIR - Thakker, Paresh IR - Thakker P FIR - Derdak, Zoltan IR - Derdak Z FIR - Landgren, Henrik IR - Landgren H FIR - Lackner, Carolin IR - Lackner C FIR - Gouw, Annette S H IR - Gouw ASH FIR - Hytiroglou, Prodromos IR - Hytiroglou P FIR - Aller, Rocio IR - Aller R FIR - Gonzalez, Rebeca Siguenza IR - Gonzalez RS FIR - Pericas, Juan M IR - Pericas JM FIR - Agustin, Salvador IR - Agustin S FIR - Rivera-Esteban, Jesus M IR - Rivera-Esteban JM FIR - Munoz-Martinez, Sergio IR - Munoz-Martinez S FIR - Jimenez-Masip, Alba M IR - Jimenez-Masip AM FIR - Pages, Laura IR - Pages L FIR - Rojo, Diego IR - Rojo D EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 19:52 PHST- 2026/01/22 00:00 [received] PHST- 2026/05/31 00:00 [revised] PHST- 2026/06/02 00:00 [accepted] PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:52 [entrez] AID - S0140-6736(26)01145-1 [pii] AID - 10.1016/S0140-6736(26)01145-1 [doi] PST - ppublish SO - Lancet. 2026 Oct 31;408(10566):1850-1865. doi: 10.1016/S0140-6736(26)01145-1. PMID- 42956762 OWN - NLM STAT- In-Process LR - 20261029 IS - 1474-547X (Electronic) IS - 0140-6736 (Linking) VI - 408 IP - 10566 DP - 2026 Oct 31 TI - Non-invasive tests in MASH: clinical practice versus trials. PG - 1794-1796 LID - S0140-6736(26)01144-X [pii] LID - 10.1016/S0140-6736(26)01144-X [doi] FAU - Gluud, Lise Lotte AU - Gluud LL AD - Gastro Unit, Copenhagen University Hospital Hvidovre, Hvidovre DK-2650, Denmark; Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark. Electronic address: lise.lotte.gluud.01@regionh.dk. FAU - Kimer, Nina AU - Kimer N AD - Gastro Unit, Copenhagen University Hospital Hvidovre, Hvidovre DK-2650, Denmark; Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark. LA - eng PT - Journal Article PL - England TA - Lancet JT - Lancet (London, England) JID - 2985213R SB - IM COIS- LLG reports grants from Novo Nordisk, Gilead, Sobi International, and Alexion; consulting fees from Novo Nordisk, AstraZeneca, Sobi, Pfizer, and Becton Dickinson; honoraria for lectures, presentations, or educational events from Novo Nordisk, AstraZeneca, Sobi International, Pfizer, Becton Dickinson, Ipsen, and Ecosense; and support for attending meetings and travel from Ipsen. NK reports grants from the Novo Nordisk Foundation and the Karen Elise Jensen Foundation, administered by her institution; honoraria from Novo Nordisk paid to her institution; and support for attending meetings and travel from Ipsen. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:52 PHST- 2026/05/22 00:00 [received] PHST- 2026/06/02 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:52 [entrez] AID - S0140-6736(26)01144-X [pii] AID - 10.1016/S0140-6736(26)01144-X [doi] PST - ppublish SO - Lancet. 2026 Oct 31;408(10566):1794-1796. doi: 10.1016/S0140-6736(26)01144-X. PMID- 42956744 OWN - NLM STAT- Publisher LR - 20261029 IS - 1875-9777 (Electronic) IS - 1875-9777 (Linking) DP - 2026 Oct 29 TI - Niche-reprogramming CAR T therapy improves skeletal muscle regeneration and function in preclinical murine models of Duchenne muscular dystrophy. LID - S1934-5909(26)00385-1 [pii] LID - 10.1016/j.stem.2026.10.004 [doi] AB - Duchenne muscular dystrophy (DMD) is a fatal neuromuscular disorder with no cure. Emerging dystrophin-restoring gene therapies target mutant myofibers but face challenges in efficacy and toxicities, underscoring the need for new treatments. Current therapies do not address chronic inflammation and muscle fibrosis, key pathologies creating a pathological muscle microenvironment that impairs dystrophic muscle function and regeneration. Here, we identify urokinase plasminogen activator receptor (uPAR) as a shared surface marker on subsets of macrophages and fibro-adipogenic progenitors exhibiting strong pro-inflammatory and fibrogenic signatures in DMD. Notably, uPAR-directed chimeric antigen receptor (CAR) T cells selectively eliminate these pathogenic subpopulations, remodel the muscle microenvironment, and enhance dystrophic muscle regeneration and force production; they also improve diaphragm and respiratory function, addressing an unmet clinical need and a major cause of DMD mortality. These findings establish uPAR CAR T cells as a niche-remodeling regenerative immunotherapy that simultaneously addresses multiple DMD pathologies and improves dystrophic muscle regeneration and function. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Afzal, Hamayl AU - Afzal H AD - Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA; Cell and Molecular Biology Program, Duke University, Durham, NC 27708, USA; Duke Regeneration Center, Duke University Medical Center, Durham, NC 27710, USA. FAU - Rotstein, Tomer AU - Rotstein T AD - Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA; Center for Advanced Genomic Technologies, Duke University, Durham, NC 27708, USA. FAU - Biswas, Debolina D AU - Biswas DD AD - Division of Pulmonary and Sleep Medicine, Department of Pediatrics, Duke University Medical Center, Durham, NC 27710, USA. FAU - Lin, Xin AU - Lin X AD - Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA. FAU - Khodabukus, Alastair AU - Khodabukus A AD - Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA. FAU - Luo, Wenzhe AU - Luo W AD - Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA. FAU - Xu, Yueyuan AU - Xu Y AD - Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA; Duke Regeneration Center, Duke University Medical Center, Durham, NC 27710, USA; Center for Advanced Genomic Technologies, Duke University, Durham, NC 27708, USA. FAU - Frair, Emma C AU - Frair EC AD - Center for Gene Therapy, Nationwide Children's Hospital, Columbus, OH 43205, USA. FAU - Li, Yongwu AU - Li Y AD - Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA. FAU - Chen, Jingjuan AU - Chen J AD - Department of Orthopaedic Surgery, Duke University Medical Center, Durham, NC 27710, USA. FAU - Kuang, Shihuan AU - Kuang S AD - Department of Orthopaedic Surgery, Duke University Medical Center, Durham, NC 27710, USA. FAU - Flanigan, Kevin M AU - Flanigan KM AD - Center for Gene Therapy, Nationwide Children's Hospital, Columbus, OH 43205, USA; Department of Pediatrics, The Ohio State University, Columbus, OH 43210, USA; Department of Neurology, The Ohio State University, Columbus, OH 43210, USA. FAU - Bursac, Nenad AU - Bursac N AD - Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA. FAU - ElMallah, Mai K AU - ElMallah MK AD - Division of Pulmonary and Sleep Medicine, Department of Pediatrics, Duke University Medical Center, Durham, NC 27710, USA. FAU - Gersbach, Charles A AU - Gersbach CA AD - Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA; Center for Advanced Genomic Technologies, Duke University, Durham, NC 27708, USA. FAU - Diao, Yarui AU - Diao Y AD - Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA; Duke Regeneration Center, Duke University Medical Center, Durham, NC 27710, USA; Center for Advanced Genomic Technologies, Duke University, Durham, NC 27708, USA; Department of Orthopaedic Surgery, Duke University Medical Center, Durham, NC 27710, USA; Department of Pathology, Duke University Medical Center, Durham, NC 27710, USA. Electronic address: yarui.diao@duke.edu. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Cell Stem Cell JT - Cell stem cell JID - 101311472 SB - IM OTO - NOTNLM OT - CAR T therapy OT - DMD OT - chronic inflammation OT - fibrosis OT - muscle microenvironment OT - muscle regeneration COIS- Declaration of interests The authors declare no competing interests. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:42 PHST- 2025/09/14 00:00 [received] PHST- 2026/08/16 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:42 [entrez] AID - S1934-5909(26)00385-1 [pii] AID - 10.1016/j.stem.2026.10.004 [doi] PST - aheadofprint SO - Cell Stem Cell. 2026 Oct 29:S1934-5909(26)00385-1. doi: 10.1016/j.stem.2026.10.004. PMID- 42956720 OWN - NLM STAT- Publisher LR - 20261029 IS - 1758-1125 (Electronic) IS - 0268-3555 (Linking) DP - 2026 Oct 29 TI - Functional phlebectomy for saphenous reflux: A retrospective cohort of 759 patients with 3-year follow-up. PG - 2683555261500594 LID - 10.1177/02683555261500594 [doi] AB - BackgroundThe optimal management of chronic venous disease with saphenous reflux remains debated. While truncal ablation is commonly recommended, haemodynamically oriented and saphenous-sparing strategies have emerged, possibly challenging the necessity of routine treatment of the saphenous vein in all patients.ObjectiveTo evaluate clinical and haemodynamic outcomes following functional phlebectomy in patients with symptomatic varicose veins and saphenous reflux, and to assess the need for subsequent truncal intervention.MethodsA retrospective cohort study was conducted including 759 consecutive patients with CEAP C2 disease and duplex-confirmed saphenous reflux, treated between 2008 and 2019. All patients underwent functional phlebectomy targeting refluxing tributaries without routine saphenous ablation. Follow-up was performed up to 36 months with clinical assessment and duplex ultrasound. Outcomes were reported descriptively as absolute numbers and percentages as collected at 36 months. Secondary staged treatment with foam sclerotherapy was performed in patients with persistent symptomatic reflux.ResultsAfter functional phlebectomy, absence of reflux was observed in 24.9% of patients, while 49.5% presented persistence of reflux without symptoms and 24.9% persistence with symptoms. Saphenous vein thrombosis occurred in 0.6% of cases. Among patients undergoing secondary sclerotherapy (n = 189), absence of reflux was achieved in 10.05%, while 39.6% became asymptomatic despite persistent reflux. Overall, only a minority of patients required subsequent treatment of the saphenous vein. Saphenous vein diameter appeared to influence treatment outcomes, with preservation observed in all cases with diameters  80% removal in real Pearl River water and meeting discharge standards. Mechanistically, we reveal a diffusion-ligand exchange-inner-sphere complexation pathway in which Zr(4+) enhances Lewis acidity and coordination affinity, while Fe-O sites play an auxiliary role, collectively accounting for the superior selectivity. This work not only provides a scalable, high-performance membrane for As(V) remediation but also offers insights into the rational design of Zr-based adsorbents for capturing toxic oxyanions. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Dong, Kaige AU - Dong K AD - College of Environment and Ecology, Taiyuan University of Technology, Taiyuan 030024, China; Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China. FAU - Yi, Zhihao AU - Yi Z AD - State Key Laboratory of Chemistry for NBC Hazards Protection, Beijing 102205, China. FAU - Mahmud, Sakil AU - Mahmud S AD - Ivory V. Nelson Center for the Sciences, Department of Chemistry and Physics, Lincoln University, 1570 Baltimore Pike, Oxford, PA 19352, United States; Department of Textile Engineering, Faculty of Engineering, Daffodil International University, Dhaka 1216, Bangladesh. FAU - Cheng, Yuhang AU - Cheng Y AD - Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China. FAU - Deng, Manyu AU - Deng M AD - Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China. FAU - Zhang, Feng AU - Zhang F AD - College of Environment and Ecology, Taiyuan University of Technology, Taiyuan 030024, China. Electronic address: zhangfeng@tyut.edu.cn. FAU - Huang, Xuexia AU - Huang X AD - Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China. FAU - Zhang, Gaosheng AU - Zhang G AD - Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China. FAU - Xiong, Zhu AU - Xiong Z AD - Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China. Electronic address: xiongzhu@gzhu.edu.cn. FAU - Zhang, Wei AU - Zhang W AD - Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China. Electronic address: zh_wei@gzhu.edu.cn. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - J Hazard Mater JT - Journal of hazardous materials JID - 9422688 SB - IM OTO - NOTNLM OT - As(V) removal OT - Composite membrane OT - Ligand exchange OT - Water treatment OT - Zr-doped beta-FeOOH COIS- Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Zhu Xiong reports financial support was provided by Guangzhou University. Zhu Xiong reports a relationship with Guangzhou University that includes:. No If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:11 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:11 [entrez] AID - S0304-3894(26)03025-6 [pii] AID - 10.1016/j.jhazmat.2026.144044 [doi] PST - aheadofprint SO - J Hazard Mater. 2026 Oct 27;518:144044. doi: 10.1016/j.jhazmat.2026.144044. PMID- 42956703 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-2456 (Electronic) IS - 0956-053X (Linking) VI - 228 DP - 2026 Oct 29 TI - Blockchain-integrated bilevel optimization for traceable healthcare waste management. PG - 115950 LID - S0956-053X(26)00620-3 [pii] LID - 10.1016/j.wasman.2026.115950 [doi] AB - Blockchain Technology (BCT) has emerged as a promising mechanism for enhancing traceability and accountability in Healthcare Waste Management (HWM) systems. This study develops a bilevel optimization model for HWM network design that explicitly incorporates blockchain adoption decisions alongside economic, environmental, and public health objectives. The proposed framework captures the hierarchical interaction between regulatory authorities and logistics operators by modeling the government as the upper-level decision-maker responsible for minimizing infection risk and carbon-related impacts, while the lower-level decision-maker minimizes operational costs through waste flow allocation, conditional on the facility configuration and blockchain investments selected by the upper-level decision-maker. Blockchain adoption is modeled as an endogenous binary decision at the hospital level, enabling the evaluation of selective traceability strategies. The model is applied to a real-world case study in Turkiye involving 23 hospitals and multiple collection and treatment facilities. Computational results demonstrate that full blockchain deployment is not always optimal; instead, partial adoption (34.8% of hospitals) achieves 79.39% network traceability while providing a balanced trade-off among cost, risk, and environmental performance. Pareto frontier analysis reveals significant trade-offs between risk reduction and environmental objectives. Furthermore, pandemic scenario analysis and viability-oriented sensitivity analyses incorporating sustainability, resilience, and agility dimensions show that blockchain adoption exhibits threshold-based behavior, increasing sharply under high operational stress and reaching full deployment under extreme conditions. The findings highlight the structural role of blockchain in reshaping Healthcare Waste (HW) network configurations and provide managerial insights for selective and context-dependent blockchain implementation strategies. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Yesilkaya, Murat AU - Yesilkaya M AD - Department of Industrial Engineering, Tokat Gaziosmanpasa University, Tokat, Turkiye. Electronic address: murat.yesilkaya@gop.edu.tr. FAU - Tirkolaee, Erfan Babaee AU - Tirkolaee EB AD - Department of Industrial Engineering, Istinye University, Istanbul, Turkiye; Universidad Espiritu Santo, Samborondon, Ecuador. Electronic address: erfan.babaee@istinye.edu.tr. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Waste Manag JT - Waste management (New York, N.Y.) JID - 9884362 SB - IM OTO - NOTNLM OT - Bilevel optimization OT - Blockchain technology OT - Healthcare waste management OT - Pandemic preparedness OT - Traceability COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:11 PHST- 2026/07/24 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:11 [entrez] AID - S0956-053X(26)00620-3 [pii] AID - 10.1016/j.wasman.2026.115950 [doi] PST - aheadofprint SO - Waste Manag. 2026 Oct 29;228:115950. doi: 10.1016/j.wasman.2026.115950. PMID- 42956696 OWN - NLM STAT- Publisher LR - 20261029 IS - 1528-3984 (Electronic) IS - 0197-4572 (Linking) VI - 74 IP - Pt B DP - 2026 Oct 29 TI - Acupuncture for postoperative pain management and functional recovery after total knee arthroplasty in older adults: a systematic review and meta-analysis. PG - 104406 LID - S0197-4572(26)00611-7 [pii] LID - 10.1016/j.gerinurse.2026.104406 [doi] AB - OBJECTIVE: To evaluate the effectiveness and safety of acupuncture for postoperative pain management and functional recovery in older adults following total knee arthroplasty (TKA). METHODS: A systematic review and meta-analysis of randomized controlled trials (RCTs) was conducted. PubMed, Embase, Web of Science, and the Cochrane Library were searched for randomized controlled trials (RCTs) evaluating acupuncture or electroacupuncture for postoperative outcomes in adults undergoing TKA. Primary outcomes included pain intensity (Visual Analogue Scale [VAS]) and functional outcomes (Western Ontario and McMaster Universities Osteoarthritis Index [WOMAC], Hospital for Special Surgery [HSS] score, and range of motion). Secondary outcomes included analgesic consumption and adverse events. RESULTS: Fifteen RCTs involving 1037 participants were included. Acupuncture and electroacupuncture were associated with reduced postoperative pain (VAS: SMD = -1.08, 95% CI, -1.27 to -0.89; Z = 11.09; p < 0.00001) and improved functional outcomes (HSS: SMD = 1.39, 95% CI, 1.02 to 1.75; Z = 7.48; p < 0.00001). No serious adverse events were reported in the included studies. CONCLUSIONS: Acupuncture may provide beneficial effects on postoperative pain and selected functional outcomes after TKA in older adults. Given the multidisciplinary nature of postoperative recovery, these findings may be relevant to geriatric nursing care, particularly in pain assessment, rehabilitation coordination, and monitoring of recovery. However, the available evidence does not establish acupuncture as a nurse-led intervention. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Kou, Haiyang AU - Kou H AD - College of Acupuncture and Tuina, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, 550025, China. FAU - Liu, Huaiquan AU - Liu H AD - College of Acupuncture and Tuina, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, 550025, China. FAU - Lai, Lingyan AU - Lai L AD - College of Acupuncture and Tuina, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, 550025, China. FAU - Yang, Shili AU - Yang S AD - College of Acupuncture and Tuina, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, 550025, China. FAU - Zhang, Xinyan AU - Zhang X AD - College of Acupuncture and Tuina, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, 550025, China. FAU - Sun, Yu AU - Sun Y AD - College of Acupuncture and Tuina, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, 550025, China. FAU - Xu, Yunlin AU - Xu Y AD - College of Acupuncture and Tuina, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, 550025, China. FAU - Chen, Bo AU - Chen B AD - College of Acupuncture and Tuina, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, 550025, China. Electronic address: dallastom@163.com. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Geriatr Nurs JT - Geriatric nursing (New York, N.Y.) JID - 8309633 SB - IM OTO - NOTNLM OT - Acupuncture OT - Nurse-led intervention OT - Older adults OT - Pain management OT - Rehabilitation OT - Total knee arthroplasty COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:10 PHST- 2026/04/01 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:10 [entrez] AID - S0197-4572(26)00611-7 [pii] AID - 10.1016/j.gerinurse.2026.104406 [doi] PST - aheadofprint SO - Geriatr Nurs. 2026 Oct 29;74(Pt B):104406. doi: 10.1016/j.gerinurse.2026.104406. PMID- 42956690 OWN - NLM STAT- Publisher LR - 20261029 IS - 1096-0341 (Electronic) IS - 0042-6822 (Linking) VI - 625 DP - 2026 Oct 15 TI - Influenza viruses as vaccine vectors: Historical background and future directions. PG - 111105 LID - S0042-6822(26)00321-1 [pii] LID - 10.1016/j.virol.2026.111105 [doi] AB - Influenza viruses have served as experimental vaccine vectors for more than three decades, combining such advantages as mucosal delivery, segmented reverse genetics and clinically established attenuation strategies. This review summarizes historical insights into vector design based on influenza A and B viruses and proposes future applications for this platform. Particularly, we suggest using a trivalent live attenuated influenza vaccine (LAIV) as a reusable seasonal multi-vector system. In this approach, the A/H1N1, A/H3N2 and influenza B vaccine strains could each carry distinct foreign antigenic cassettes from multiple human respiratory viruses. Annual strain replacement, already intrinsic to influenza vaccination, would then also provide repeated mucosal boosting against selected non-influenza pathogens. For influenza A viruses, a substantial body of knowledge has been accumulated regarding the design of chimeric constructs carrying heterologous antigenic cassettes derived from other viruses or bacteria. Extensive animal studies and clinical trials have confirmed the functionality of this platform and underscored the promise of attenuated influenza A viruses as delivery vehicles for target antigens, capable of eliciting robust immunity. In contrast, data on influenza B virus-based vectors remain scarce and fragmented. However, given that influenza B strains in trivalent LAIVs are particularly potent inducers of immune responses, further development of this vector platform could not only overcome the packaging constraints of the influenza genome for foreign inserts but also enable the creation of effective multivalent vaccines against the most epidemiologically relevant respiratory pathogens. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Isakova-Sivak, Irina AU - Isakova-Sivak I AD - Institute of Experimental Medicine, Saint Petersburg, Russia. Electronic address: isakova.sivak@iemspb.ru. FAU - Matyushenko, Victoria AU - Matyushenko V AD - Institute of Experimental Medicine, Saint Petersburg, Russia. FAU - Rudenko, Larisa AU - Rudenko L AD - Institute of Experimental Medicine, Saint Petersburg, Russia. LA - eng PT - Journal Article PT - Review DEP - 20261015 PL - United States TA - Virology JT - Virology JID - 0110674 SB - IM OTO - NOTNLM OT - Annual vaccination OT - Influenza B OT - Influenza a OT - Influenza virus OT - Live attenuated influenza vaccine OT - Mucosal immunity OT - Reverse genetics OT - Trivalent vaccine OT - Vector vaccine OT - Viral vector COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Larisa Rudenko reports financial support was provided by Russian Science Foundation. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:10 PHST- 2026/07/31 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:10 [entrez] AID - S0042-6822(26)00321-1 [pii] AID - 10.1016/j.virol.2026.111105 [doi] PST - aheadofprint SO - Virology. 2026 Oct 15;625:111105. doi: 10.1016/j.virol.2026.111105. PMID- 42956688 OWN - NLM STAT- Publisher LR - 20261029 IS - 1878-5891 (Electronic) IS - 0378-5955 (Linking) VI - 483 DP - 2026 Oct 20 TI - Persistent auditory-nerve deficits and shifted spontaneous-rate composition following noise-induced temporary threshold shift in chinchillas. PG - 109842 LID - S0378-5955(26)00313-8 [pii] LID - 10.1016/j.heares.2026.109842 [doi] AB - Listeners with clinically normal audiograms can still experience difficulty hearing in noise, a problem often discussed in the context of "hidden hearing loss". Cochlear synaptopathy has been proposed as one potential mechanism underlying these deficits, but reliable physiological assays for detecting synaptopathy remain challenging. The present study examined the effects of noise exposure that produced temporary threshold shift (TTS) in chinchillas using cochlear histology, noninvasive physiological measures, and single-unit auditory-nerve recordings. Histology confirmed persistent loss of inner-hair-cell synapses following exposure. ABR thresholds recovered and DPOAE levels were preserved 2 weeks after exposure, and many auditory-nerve response properties, including tuning and rate-level response metrics, showed no significant post-exposure differences among surviving fibers. However, several suprathreshold and timing-related abnormalities persisted after exposure. Suprathreshold ABR measures showed reduced Peak 1 amplitudes and prolonged Peak 5-Peak 1 latencies, while single-unit recordings revealed a reduced proportion of low-SR fibers in the exposed population. Selective post-exposure differences were also observed in auditory-nerve timing and modulation-related metrics, including reduced first-spike timing variability, increased adapted firing rate, reduced envelope-coding strength, and prolonged envelope-coding delay, suggesting residual disruption of suprathreshold temporal coding that may be perceptually relevant for listening in noise. These findings suggest that recovered TTS produces subtle but persistent neural changes that are not fully captured by conventional threshold-based measures. The results may help explain why diagnosis of cochlear synaptopathy remains challenging, and support the use of suprathreshold, latency-sensitive, and modulation-based physiological assays to detect residual neural effects of cochlear synaptopathy. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Farhadi, Afagh AU - Farhadi A AD - Department of Speech, Language, and Hearing Sciences, Purdue University, West Lafayette, IN, 47907, USA. FAU - Muthaiah, Vijaya Prakash Krishnan AU - Muthaiah VPK AD - Department of Speech, Language, and Hearing Sciences, Purdue University, West Lafayette, IN, 47907, USA. FAU - Heinz, Michael G AU - Heinz MG AD - Department of Speech, Language, and Hearing Sciences, Purdue University, West Lafayette, IN, 47907, USA; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA. Electronic address: mheinz@purdue.edu. LA - eng PT - Journal Article DEP - 20261020 PL - Netherlands TA - Hear Res JT - Hearing research JID - 7900445 SB - IM OTO - NOTNLM OT - Auditory nerve OT - Cochlear synaptopathy OT - Envelope coding OT - Hidden hearing loss OT - Noise exposure OT - Temporary threshold shift COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:10 PHST- 2026/07/11 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:10 [entrez] AID - S0378-5955(26)00313-8 [pii] AID - 10.1016/j.heares.2026.109842 [doi] PST - aheadofprint SO - Hear Res. 2026 Oct 20;483:109842. doi: 10.1016/j.heares.2026.109842. PMID- 42956687 OWN - NLM STAT- Publisher LR - 20261029 IS - 1878-5891 (Electronic) IS - 0378-5955 (Linking) VI - 483 DP - 2026 Oct 23 TI - Exploring tinnitus-like central hyperactivity in a large language model-based computational analog of hearing loss. PG - 109845 LID - S0378-5955(26)00316-3 [pii] LID - 10.1016/j.heares.2026.109845 [doi] AB - Tinnitus is commonly associated with maladaptive neural plasticity following hearing loss. Although large language models (LLMs) share similarities with the human brain in language processing and representation, their potential for modeling tinnitus-related neural mechanisms remains limited. This study aimed to develop an LLM-based framework to investigate tinnitus-related maladaptive neural plasticity. An LLM was partitioned into eight independently trainable regions. An exploratory computational analogy of deafferentation-related changes associated with peripheral hearing loss was developed by applying token-specific high-frequency attenuation during training on a commonsense reasoning task. Region-specific contributions to tinnitus-like excess activity were quantified using region-wise plasticity ablation, selectively disabling plasticity in one region at a time. Representational relationships among regions were evaluated using centered kernel alignment. The computational deafferentation analog largely preserved task accuracy but showed increased activity. Region 1 was the only region with a net suppressive contribution to excess activity, whereas region 2 exhibited the largest positive contribution; regions 6 and 8 also contributed positively. Regions 1 and 2 remained representationally distinct across all conditions. Deafferentation tended to reduce region 1-region 2 similarity, whereas plasticity ablation in most regions, except for regions 6 and 8, tended to increase it. The proposed LLM-based framework captured network-level functional dissociations consistent with current theories of hearing loss-associated tinnitus, identifying distinct suppressive, transformation, and higher-order modulatory roles. These findings highlight the potential of LLMs to investigate maladaptive neural changes underlying tinnitus. This framework may provide the foundation for constructing patient-specific digital twins. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Han, Sang-Yoon AU - Han SY AD - Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Hanyang University, Seoul, Republic of Korea. FAU - Song, Jae-Jin AU - Song JJ AD - Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, Seoul, Republic of Korea. FAU - Choi, Byung Yoon AU - Choi BY AD - Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Bundang Hospital, Seongnam, Republic of Korea. FAU - Koo, Ja-Won AU - Koo JW AD - Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Bundang Hospital, Seongnam, Republic of Korea. FAU - Chang, Munyoung AU - Chang M AD - Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Bundang Hospital, Seongnam, Republic of Korea. Electronic address: caduent@gmail.com. LA - eng PT - Journal Article DEP - 20261023 PL - Netherlands TA - Hear Res JT - Hearing research JID - 7900445 SB - IM OTO - NOTNLM OT - Auditory pathways OT - Deep learning OT - Hearing loss OT - Large language models OT - Tinnitus COIS- Declaration of competing interest The authors declare no competing interests. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:10 PHST- 2026/08/04 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:10 [entrez] AID - S0378-5955(26)00316-3 [pii] AID - 10.1016/j.heares.2026.109845 [doi] PST - aheadofprint SO - Hear Res. 2026 Oct 23;483:109845. doi: 10.1016/j.heares.2026.109845. PMID- 42956686 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-2057 (Electronic) IS - 0001-4575 (Linking) VI - 240 DP - 2026 Oct 29 TI - What matters in pre-crash driving behavior modeling? An evaluation of state representations via imitation learning. PG - 108840 LID - S0001-4575(26)00449-5 [pii] LID - 10.1016/j.aap.2026.108840 [doi] AB - Investigating pre-crash driving behaviors is vital for uncovering human responses to immediate threats. However, current research mainly relies on naturalistic driving data, capturing only non-critical behavioral patterns. More importantly, the fundamental impact of state representation design on the fidelity of behavioral learning remains largely unexplored. To address these gaps, this study conducts systematic state representation engineering for pre-crash scenarios. We formulate four progressive state representations, evolving from basic geometric coordinates to a comprehensive risk-aware formulation. To evaluate their impact, these representations are integrated into three distinct imitation learning (IL) algorithms to model behavioral patterns from real-world pre-crash trajectory data. The trained policies are subsequently evaluated in a simulated interactive environment: the agent controls the ego vehicle to interact with an object vehicle that replays testing-set trajectories, allowing for a direct comparison of maneuvers against authentic human responses. Experimental results demonstrate that while algorithmic selection influences performance, the state representation plays also a significantly decisive role. Across all three IL algorithms, agents utilizing the risk-aware representation consistently generate maneuvers most similar to those of the real-world ego vehicle. Furthermore, an analysis of the extracted behavioral rewards indicates that the risk-aware representation enables the agent to internalize human driving logic, moving beyond the mere mechanical replication of observed trajectories. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Liu, Min AU - Liu M AD - School of Automation, Changsha, 410075, Hunan, China. FAU - Zhou, Hanchu AU - Zhou H AD - School of Traffic and Transportation Engineering, Changsha, 410075, Hunan, China. FAU - Yang, Zimo AU - Yang Z AD - School of Traffic and Transportation Engineering, Changsha, 410075, Hunan, China. FAU - Gui, Gui AU - Gui G AD - School of Automation, Changsha, 410075, Hunan, China. FAU - Bian, Jiang AU - Bian J AD - School of Traffic and Transportation Engineering, Changsha, 410075, Hunan, China. Electronic address: bianjiang@csu.edu.cn. FAU - Zhou, Rui AU - Zhou R AD - School of Automation, Changsha, 410075, Hunan, China. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Accid Anal Prev JT - Accident; analysis and prevention JID - 1254476 SB - IM OTO - NOTNLM OT - Behavior modeling OT - Imitation learning OT - Pre-crash OT - Traffic safety COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:10 PHST- 2026/05/06 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:10 [entrez] AID - S0001-4575(26)00449-5 [pii] AID - 10.1016/j.aap.2026.108840 [doi] PST - aheadofprint SO - Accid Anal Prev. 2026 Oct 29;240:108840. doi: 10.1016/j.aap.2026.108840. PMID- 42956685 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-2057 (Electronic) IS - 0001-4575 (Linking) VI - 240 DP - 2026 Oct 29 TI - Configuring hidden and visible camera enforcement for risky driving deterrence. PG - 108839 LID - S0001-4575(26)00448-3 [pii] LID - 10.1016/j.aap.2026.108839 [doi] AB - Road rule violations such as speeding and mobile phone use while driving remain major contributors to road crashes worldwide. Camera-based enforcement is widely implemented to address these behaviours, yet the relative and combined effects of overt and covert enforcement remain insufficiently understood. This investigation develops and tests a deterrence-based framework for identifying the perceived effectiveness of hidden and visible camera enforcement, focusing on key mechanisms including perceived certainty of apprehension and punishment avoidance. A cross-sectional, scenario-based survey was conducted with 1,167 licensed drivers in Australia. Participants evaluated four enforcement configurations (no enforcement, covert cameras, overt cameras, and combined covert and overt cameras). Outcomes included deterrence perceptions, willingness to engage in offending behaviours, and attitudes towards enforcement. Ordinal random forest models were used for variable selection, followed by cumulative link mixed-effects models to estimate effects while accounting for repeated measures. Covert enforcement was associated with higher perceived certainty of apprehension, whereas overt enforcement reduced punishment avoidance and willingness to offend. Punishment avoidance emerged as a central behavioural mechanism, showing stronger associations with willingness than perceived certainty. Combined enforcement aligned these mechanisms, increasing perceived certainty while reducing opportunities for undetected violations. Visible enforcement was also associated with more positive attitudes, indicating a complementary role in supporting legitimacy. The findings demonstrate that deterrence is not a property of individual enforcement mechanisms, but an emergent outcome of how enforcement is configured. Effective strategies should therefore balance uncertainty, visibility, and communication across the road network. These results extend deterrence theory by showing that key mechanisms are co-produced through enforcement configurations and provide practical guidance for designing more effective and publicly acceptable enforcement systems. CI - Copyright (c) 2026 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Truelove, Verity AU - Truelove V AD - MAIC/University of the Sunshine Coast Road Safety Research Collaboration, School of Law and Society, 90 Sippy Downs Dr, Sippy Downs, Queensland, 4556, Australia. Electronic address: vtruelove@usc.edu.au. FAU - Oviedo-Trespalacios, Oscar AU - Oviedo-Trespalacios O AD - Department of Values, Technology & Innovation, Delft University of Technology, Delft, the Netherlands; Cho Chun Shik Graduate School of Mobility, Korea Advanced Institute of Science & Technology (KAIST), Daejeon, South Korea. FAU - Larue, Gregoire S AU - Larue GS AD - MAIC/University of the Sunshine Coast Road Safety Research Collaboration, School of Law and Society, 90 Sippy Downs Dr, Sippy Downs, Queensland, 4556, Australia. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Accid Anal Prev JT - Accident; analysis and prevention JID - 1254476 SB - IM OTO - NOTNLM OT - Camera enforcement OT - Deterrence OT - Enforcement configuration OT - Mobile phone use OT - Punishment avoidance OT - Speeding COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:10 PHST- 2026/05/06 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:10 [entrez] AID - S0001-4575(26)00448-3 [pii] AID - 10.1016/j.aap.2026.108839 [doi] PST - aheadofprint SO - Accid Anal Prev. 2026 Oct 29;240:108839. doi: 10.1016/j.aap.2026.108839. PMID- 42956682 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-2219 (Electronic) IS - 0966-6362 (Linking) VI - 132 DP - 2026 Oct 23 TI - Effects of cues to walk softly and quietly on gait mechanics during load carriage. PG - 110676 LID - S0966-6362(26)00587-4 [pii] LID - 10.1016/j.gaitpost.2026.110676 [doi] AB - Walking with load carriage is a routine and necessary activity for many occupations and recreational athletes, however it induces significant physical strain, resulting in high rates of musculoskeletal injury. Gait alteration cues to run softly/quietly have been shown to reduce external forces related to injury in runners. However, the effects of these cues have yet to be explored for load carriage. 38 participants completed testing on an instrumented treadmill while kinematics were tracked via optical motion capture. Participants first completed a habitual load carriage trial (HAB) at a self-selected pace (1.23 +/- 0.12 m/s) while carrying an 18 kg weighted vest. They were given a 5-min warm-up followed by data collection. Participants were then cued to modify their gait by walking "quietly and softly" (SOFT), using the same pace as for HAB. Outcome variables were averaged across 20 strides, including peak hip/knee/ankle joint internal sagittal and frontal plane moments, peaks/excursion in joint angles, double-stance time, stride length, and cadence. The SOFT condition resulted in decreased second peak hip abduction and peak knee flexion moments, and second peak vertical forces, but increased peak knee extension moments and first peak vertical forces (p < 0.05, Cohen's d>/=0.5). Further, the SOFT condition resulted in increased peak flexion angles (hip/knee) and reduced extension/plantarflexion angles (hip/knee/ankle). Given the conflicting changes in external forces, the results do not support SOFT as a modification to reduce overall musculoskeletal injury risk during load carriage. CI - Published by Elsevier B.V. FAU - Johnson, Caleb D AU - Johnson CD AD - US Army Institute of Environmental Medicine, 10 General Greene Ave, Natick, MA, 01760, USA. Electronic address: caleb.d.johnson24.civ@health.mil. FAU - Zeppetelli, David J AU - Zeppetelli DJ AD - US Army Institute of Environmental Medicine, 10 General Greene Ave, Natick, MA, 01760, USA. FAU - Dempsey, Cole A AU - Dempsey CA AD - US Army Institute of Environmental Medicine, 10 General Greene Ave, Natick, MA, 01760, USA. FAU - Sarantos, Lucas AU - Sarantos L AD - US Army Institute of Environmental Medicine, 10 General Greene Ave, Natick, MA, 01760, USA. FAU - Sara, Lauren K AU - Sara LK AD - School of Rehabilitation Sciences & Technology, University of Wisconsin-Milwaukee, 3203 N Downer Ave, Milwaukee, WI, 53211, USA. LA - eng PT - Journal Article DEP - 20261023 PL - England TA - Gait Posture JT - Gait & posture JID - 9416830 SB - IM OTO - NOTNLM OT - Gait modification OT - Gait retraining OT - Ground reaction forces OT - Joint kinematics OT - Joint kinetics OT - Military COIS- Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Caleb Johnson reports financial support was provided by US Army Medical Research and Development Command. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:10 PHST- 2026/06/11 00:00 [received] PHST- 2026/09/09 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:10 [entrez] AID - S0966-6362(26)00587-4 [pii] AID - 10.1016/j.gaitpost.2026.110676 [doi] PST - aheadofprint SO - Gait Posture. 2026 Oct 23;132:110676. doi: 10.1016/j.gaitpost.2026.110676. PMID- 42956679 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-3557 (Electronic) IS - 1386-1425 (Linking) VI - 367 IP - Pt 2 DP - 2026 Oct 16 TI - Smartphone-assisted test paper platform based on a novel 3D Ag-MOF for facile detection of nitrofurantoin and tetracycline antibiotics. PG - 128984 LID - S1386-1425(26)01555-6 [pii] LID - 10.1016/j.saa.2026.128984 [doi] AB - Excessive use of nitrofurantoin (NFT) and tetracycline (TC) in animal-derived foods poses serious health risks, underscoring the urgent need for straightforward yet highly sensitive analytical tools. Herein, a novel 3D Ag(I) metal-organic framework (MOF) [Ag(9)(bpy)(9)(pdc)(3)].3NO(3).22H(2)O(n) (Ag-MOF-1) was obtained, which exhibits a novel five-nodal (3,3,3,4,4)-connected new topology. Ag-MOF-1 exhibits excellent fluorescence quenching responses toward NFT and TC with calculated limits of detection (LOD) as 0.737 muM for NFT and 0.864 muM for TC. A systematic study elucidates multiple fluorescence quenching mechanisms arising from host-guest interactions of Ag-MOF-1 with NFT and TC. Using its excellent sensing capabilities, we detected these antibiotic residues in five real food matrices: milk, honey, pork, fish, and eggs. A portable smartphone-integrated paper sensor was fabricated for on-site analysis, achieving satisfactory recoveries of 95.47-106.16%. This study reports a bifunctional fluorescent sensor for selective detection of NFT and TC, providing a promising, cost-effective proof-of-concept platform for food safety surveillance. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Yang, Yi AU - Yang Y AD - College of Chemistry and Materials Science, Hengyang Normal University, Hengyang, Hunan 421008, PR China. FAU - Shen, Qiu-Feng AU - Shen QF AD - College of Chemistry and Materials Science, Hengyang Normal University, Hengyang, Hunan 421008, PR China. FAU - Wu, Qiu-Hong AU - Wu QH AD - College of Chemistry and Materials Science, Hengyang Normal University, Hengyang, Hunan 421008, PR China. FAU - Zeng, Xin AU - Zeng X AD - College of Chemistry and Materials Science, Hengyang Normal University, Hengyang, Hunan 421008, PR China. FAU - Cai, Li-Qian AU - Cai LQ AD - College of Chemistry and Materials Science, Hengyang Normal University, Hengyang, Hunan 421008, PR China. FAU - Xu, Miao-Yu AU - Xu MY AD - College of Chemistry and Materials Science, Hengyang Normal University, Hengyang, Hunan 421008, PR China. FAU - Qian, Shi-Zhen AU - Qian SZ AD - College of Chemistry and Materials Science, Hengyang Normal University, Hengyang, Hunan 421008, PR China. FAU - Liu, Yang AU - Liu Y AD - College of Chemistry and Materials Science, Hengyang Normal University, Hengyang, Hunan 421008, PR China; Key Laboratory of Functional Metal-organic Compounds of Hunan Province, Hengyang, Hunan 421008, PR China; Key Laboratory of Organometallic New Materials, College of Hunan Province, Hengyang, Hunan 421008, PR China; Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River, Hengyang, Hunan 421008, PR China. FAU - Chen, Man-Sheng AU - Chen MS AD - College of Chemistry and Materials Science, Hengyang Normal University, Hengyang, Hunan 421008, PR China; Key Laboratory of Functional Metal-organic Compounds of Hunan Province, Hengyang, Hunan 421008, PR China; Key Laboratory of Organometallic New Materials, College of Hunan Province, Hengyang, Hunan 421008, PR China; Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River, Hengyang, Hunan 421008, PR China. FAU - Fu, Wei-Wei AU - Fu WW AD - College of Chemistry and Materials Science, Hengyang Normal University, Hengyang, Hunan 421008, PR China; Key Laboratory of Functional Metal-organic Compounds of Hunan Province, Hengyang, Hunan 421008, PR China; Key Laboratory of Organometallic New Materials, College of Hunan Province, Hengyang, Hunan 421008, PR China; Hunan Provincial Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River, Hengyang, Hunan 421008, PR China. Electronic address: w.w.fu@hynu.edu.cn. LA - eng PT - Journal Article DEP - 20261016 PL - England TA - Spectrochim Acta A Mol Biomol Spectrosc JT - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JID - 9602533 SB - IM OTO - NOTNLM OT - Ag(I) metal-organic framework OT - Fluorescence sensing OT - Nitrofurantoin OT - Smartphone detection OT - Test strip OT - Tetracycline COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:10 PHST- 2026/08/02 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:10 [entrez] AID - S1386-1425(26)01555-6 [pii] AID - 10.1016/j.saa.2026.128984 [doi] PST - aheadofprint SO - Spectrochim Acta A Mol Biomol Spectrosc. 2026 Oct 16;367(Pt 2):128984. doi: 10.1016/j.saa.2026.128984. PMID- 42956678 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-3557 (Electronic) IS - 1386-1425 (Linking) VI - 367 IP - Pt 2 DP - 2026 Oct 23 TI - Quinoxaline-mediated backbone modification Tunes Fe(3+)-selective versus Fe(3+)/Cu(2+) dual spectral response in Schiff-Base UV-vis probes. PG - 129042 LID - S1386-1425(26)01613-6 [pii] LID - 10.1016/j.saa.2026.129042 [doi] AB - Distinguishing Fe(3+) from Cu(2+) is challenging, as both cations exhibit similar coordination preferences toward N/O-rich chelating sites. Herein, two structurally related Schiff-base chemosensors (TPCN and TPQCN) with identical chelating motifs but modified pi-backbones were constructed to probe backbone-dependent metal-ion sensing. The short-pi-backbone TPCN displays selective absorbance turn-on for Fe(3+) with a limit of detection (LOD) of 2.28 muM. Upon incorporation of a quinoxaline moiety, TPQCN exhibits dual spectral responsiveness toward Fe(3+) and Cu(2+); however, its Cu(2+)-related response is considerably weaker, accompanied by reduced sensitivity (LODs = 8.91 muM for Fe(3+) and 23.54 muM for Cu(2+)) and compromised anti-interference capability, and TPQCN cannot discriminate the two ions in mixtures. Reliable association constants could not be determined because of incomplete spectral saturation and secondary-species formation at high metal-ion equivalents; fitted apparent values are provided in the Supporting Information for reference only and should not be used for quantitative comparison. DFT calculations on the free probes indicate localized frontier orbitals in TPCN and a more delocalized electron distribution in TPQCN, providing only qualitative mechanistic insight, as metal-complex optimizations were not performed. TPCN was evaluated in tap-water and lake-water matrices, affording spike recoveries of 95-109%. Overall, quinoxaline-mediated backbone modification (including pi-extension, altered rigidity, and electronic redistribution) shifts the sensing behavior from Fe(3+)-selective to Fe(3+)/Cu(2+) dual-spectral response. This gain in multi-ion responsiveness comes at the expense of sensitivity and ion-selectivity rather than achieving Fe(3+)/Cu(2+) discrimination, revealing a practical design trade-off for probes targeting chemically similar metal ions. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Chen, Zhen-E AU - Chen ZE AD - School of Chemistry and Chemical Engineering, Academician Workstation, Zunyi Normal College, Zunyi, 563006, China. Electronic address: zhenechen@vip.sina.com. FAU - Guo, Wei AU - Guo W AD - School of Chemistry and Chemical Engineering, Academician Workstation, Zunyi Normal College, Zunyi, 563006, China. FAU - Chen, Qiu-Feng AU - Chen QF AD - School of Chemistry and Chemical Engineering, Academician Workstation, Zunyi Normal College, Zunyi, 563006, China. FAU - Gao, Ya-Di AU - Gao YD AD - School of Chemistry and Chemical Engineering, Academician Workstation, Zunyi Normal College, Zunyi, 563006, China. FAU - Zhang, Hai AU - Zhang H AD - School of Chemistry and Chemical Engineering, Academician Workstation, Zunyi Normal College, Zunyi, 563006, China. Electronic address: haizhang@vip.sina.com. LA - eng PT - Journal Article DEP - 20261023 PL - England TA - Spectrochim Acta A Mol Biomol Spectrosc JT - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JID - 9602533 SB - IM OTO - NOTNLM OT - Metal-ion selectivity OT - Quinoxaline OT - Schiff-base probes OT - UV-vis spectroscopy OT - Pi-Conjugation effect COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:10 PHST- 2026/07/29 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:10 [entrez] AID - S1386-1425(26)01613-6 [pii] AID - 10.1016/j.saa.2026.129042 [doi] PST - aheadofprint SO - Spectrochim Acta A Mol Biomol Spectrosc. 2026 Oct 23;367(Pt 2):129042. doi: 10.1016/j.saa.2026.129042. PMID- 42956677 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-3557 (Electronic) IS - 1386-1425 (Linking) VI - 367 IP - Pt 2 DP - 2026 Oct 24 TI - Photophysics and anticancer potential of ruthenium(II) coordination compounds with 4-phenylacetylene substituted 2,6-bis(thiazol-2-yl)pyridine. PG - 128999 LID - S1386-1425(26)01570-2 [pii] LID - 10.1016/j.saa.2026.128999 [doi] AB - Ruthenium(II) coordination compounds represent a well-established platform for the investigation of triplet metal-to-ligand charge transfer (MLCT) emission properties, which are of great interest due to a variety of applications. On the other hand, ruthenium(II) complexes have attracted significant attention in anticancer research due to their unique chemical properties. Several Ru(II)-based compounds have demonstrated promising therapeutic potential, showing selective cytotoxicity toward cancer cells, reduced side effects compared to platinum drugs, and the ability to induce apoptosis through multiple pathways. In this work, a new bis-homoleptic Ru(II) polypyridyl complex incorporating 4-phenylacetylene-2,6-bis(thiazol-2-yl)pyridine (pH-CC-dtpy), [Ru(pH-CC-dtpy)(2)](CF(3)SO(3))(2) Ru_A, was synthesized and comprehensively characterized. The complex was obtained in good yield and confirmed by elemental analysis, HR-MS, FT-IR, and NMR spectroscopy, while thermogravimetric analysis revealed high thermal stability. UV-Vis spectroscopy showed intense ligand-centered transitions and metal-to-ligand charge transfer (MLCT) bands in the visible region, supported by TD-DFT calculations. The compound exhibits weak emission at room temperature and long-lived emission in a rigid matrix, characteristic of the (3)MLCT state. Importantly, the complex is stable under physiological conditions and demonstrates efficient cellular uptake. Biological studies revealed selective cytotoxicity toward 4 T1 cancer cells compared to non-tumorigenic EpH4-Ev cells, highlighting its potential as a promising scaffold for further development of Ru(II)-based anticancer agents. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Zowislok, Bartosz AU - Zowislok B AD - Institute of Chemistry, University of Silesia, 9 Szkolna Str., Katowice, 40-006, Poland. Electronic address: anna.switlicka@us.edu.pl. FAU - Wojkowska, Dagmara AU - Wojkowska D AD - Department of Hemostasis and Hemostatic Disorders, Medical University of Lodz, Mazowiecka 6/8, 92-215 Lodz, Poland. FAU - Maron, Anna Maria AU - Maron AM AD - Institute of Chemistry, University of Silesia, 9 Szkolna Str., Katowice, 40-006, Poland. FAU - Erfurt, Karol AU - Erfurt K AD - Department of Chemical Organic Technology and Petrochemistry, Silesian University of Technology, Krzywoustego 4 Street, 44-100 Gliwice, Poland. FAU - Luzak, Boguslawa AU - Luzak B AD - Department of Hemostasis and Hemostatic Disorders, Medical University of Lodz, Mazowiecka 6/8, 92-215 Lodz, Poland. Electronic address: boguslawa.luzak@umed.lodz.pl. FAU - Switlicka, Anna AU - Switlicka A AD - Institute of Chemistry, University of Silesia, 9 Szkolna Str., Katowice, 40-006, Poland. Electronic address: anna.switlicka@us.edu.pl. LA - eng PT - Journal Article DEP - 20261024 PL - England TA - Spectrochim Acta A Mol Biomol Spectrosc JT - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JID - 9602533 SB - IM OTO - NOTNLM OT - Anticancer activity OT - MLCT emission OT - Polypyridyl ligands OT - Ruthenium(II) complexes COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:10 PHST- 2026/04/21 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:10 [entrez] AID - S1386-1425(26)01570-2 [pii] AID - 10.1016/j.saa.2026.128999 [doi] PST - aheadofprint SO - Spectrochim Acta A Mol Biomol Spectrosc. 2026 Oct 24;367(Pt 2):128999. doi: 10.1016/j.saa.2026.128999. PMID- 42956674 OWN - NLM STAT- Publisher LR - 20261029 IS - 1872-8332 (Electronic) IS - 0169-5002 (Linking) VI - 222 DP - 2026 Oct 23 TI - Lung cancer associated with cystic airspaces (LCCA): A comprehensive literature review undertaken as part of the British thoracic oncology group (BTOG) work programme. PG - 109651 LID - S0169-5002(26)00712-9 [pii] LID - 10.1016/j.lungcan.2026.109651 [doi] AB - Lung cancer associated with cystic airspaces (LCCA) is an increasingly recognised yet under-characterised presentation of non-small cell lung cancer, accounting for approximately 1-4% of cases. Despite growing awareness driven by thin-section computed tomography and lung cancer screening, LCCA remains absent from major international guidelines and classifications, including those of the National Institute for Health and Care Excellence, the International Association for the Study of Lung Cancer, and the World Health Organization. This narrative review synthesises the available evidence to define LCCA as a distinct clinico-radiological entity and to inform future guideline development. LCCA presents heterogeneously, with thin- or thick walled cysts, unilocular or multiloculated morphology, and variable solid components, frequently mimicking benign cysts, bullae, or emphysema. Such ambiguity contributes to diagnostic delay; in the NELSON screening trial, LCCA accounted for 22.7% of missed or delayed diagnoses. Several radiological classifications, notably that of Mascalchi, aid description and risk stratification. Conventional staging is problematic, as measurement of the solid component alone may underestimate true tumour extent, while fluorodeoxyglucose positron emission tomography carries a high false-negative rate. Tissue sampling is similarly complicated by low diagnostic yield and false negative results, underscoring the importance of multidisciplinary team discussion. The natural history follows a stepwise progression from thin-walled cyst to wall thickening, mural nodularity, and invasive disease, with prognosis determined principally by stage, although mural nodularity and increasing solid components confer higher recurrence risk. Treatment broadly mirrors that of solid lung tumours; current guidance advocates targeting the whole cystic lesion for radical therapy rather than the solid component alone, with systemic anticancer therapy appropriate in advanced or multifocal disease. Much current practice is extrapolated from part-solid tumours; consensus on Powered by Editorial Manager(R) and ProduXion Manager(R) from Aries Systems Corporation terminology, classification, and staging, supported by prospective registry data, is required to optimise outcomes. CI - Copyright (c) 2026 The Author(s). Published by Elsevier B.V. All rights reserved. FAU - Kimani, Linda AU - Kimani L AD - Derriford Hospital, United Kingdom. FAU - Hyde, Georgia AU - Hyde G AD - Sheffield Teaching Hospitals NHS Foundation Trust, United Kingdom. FAU - O'Dowd, Emma AU - O'Dowd E AD - Nottingham University Hospitals NHS Trust, United Kingdom. FAU - Johns, Christopher AU - Johns C AD - Sheffield Teaching Hospitals NHS Foundation Trust, United Kingdom. FAU - Fitzmaurice, Gerard J AU - Fitzmaurice GJ AD - St James's Hospital, Ireland. FAU - Kar, Ashok AU - Kar A AD - Royal Infirmary of Edinburgh, United Kingdom. FAU - Majid, Muhammad AU - Majid M AD - University Hospitals of Leicester NHS Trust, United Kingdom. FAU - Jayaprakash, Kamalram Thippu AU - Jayaprakash KT AD - Cambridge University Hospitals NHS Foundation Trust, United Kingdom. FAU - Sharman, Anna AU - Sharman A AD - Manchester University NHS Foundation Trust, United Kingdom. FAU - Evison, Matthew AU - Evison M AD - Manchester University NHS Foundation Trust, United Kingdom. FAU - Bibby, Anna AU - Bibby A AD - University of Bristol, Bristol, United Kingdom. Electronic address: anna.bibby@bristol.ac.uk. LA - eng PT - Journal Article PT - Review DEP - 20261023 PL - Ireland TA - Lung Cancer JT - Lung cancer (Amsterdam, Netherlands) JID - 8800805 SB - IM OTO - NOTNLM OT - Cavitary lung cancer OT - Computed tomography OT - Cystic airspaces OT - Cystic lung cancer OT - Lung adenocarcinoma OT - Lung cancer associated with cystic airspaces OT - Lung cancer screening OT - Lung cancer staging OT - Non-small cell lung cancer OT - Pulmonary cysts COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:10 PHST- 2026/06/29 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:10 [entrez] AID - S0169-5002(26)00712-9 [pii] AID - 10.1016/j.lungcan.2026.109651 [doi] PST - aheadofprint SO - Lung Cancer. 2026 Oct 23;222:109651. doi: 10.1016/j.lungcan.2026.109651. PMID- 42956669 OWN - NLM STAT- Publisher LR - 20261029 IS - 1768-3254 (Electronic) IS - 0223-5234 (Linking) VI - 321 IP - Pt 3 DP - 2026 Oct 27 TI - Reimagining natural products: Tools and approaches to create new chemical entities for drug discovery. PG - 119471 LID - S0223-5234(26)00916-5 [pii] LID - 10.1016/j.ejmech.2026.119471 [doi] AB - Natural products (NPs) remain a valuable source of bioactive scaffolds in drug discovery. Yet, the structural complexity of NPs frequently limits their direct clinical translation. This review provides a critical, strategy-organized synthesis of the chemical toolkit used to reengineer natural product scaffolds into new chemical entities. The major strategies suggested are ring distortion, late-stage C-H functionalization, cross-coupling, and click chemistry. To map the current state of the field, we analyzed approximately 300 natural product reengineering studies published between 2015 and 2025 and found that terpenoids and alkaloids account for most reported efforts. It was also clear that late-stage functionalization substantially outnumbers scaffold-level modification across nearly all natural product classes surveyed, highlighting where future methodological development is most needed. Two documented examples illustrate the outcome of these strategies. Diels-Alder cycloaddition of a pleuromutilin-derived intermediate gave ferroptocide 3, lowering the IC(50) from 6.7 to 1.6 muM. Likewise, late-stage esterification of yohimbic acid gave a yohimbine-derived alpha2A-adrenergic receptor antagonist with 6-fold and 24-fold improved selectivity indices. We conclude with two original, prospective case studies (geldanamycin and anthracimycin) in which we apply the reviewed strategies to propose structural diversification routes for these pharmacologically significant but comparatively underexploited scaffolds. These proposed transformations have not been experimentally validated. They are presented as a strategic roadmap to illustrate the applicability of the reviewed toolkit. Together, this review offers synthetic and medicinal chemists a practical, transferable framework for natural product diversification, and identifies concrete, underexplored opportunities for future investigation. CI - Copyright (c) 2026 Elsevier Masson SAS. All rights reserved. FAU - Emad, Atef AU - Emad A AD - Department of Chemistry, Faculty of Science, Al-Azhar University, Assiut, Egypt. FAU - Beshay, Anglo AU - Beshay A AD - Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, 21511, Egypt. FAU - Hamdi, Samar AU - Hamdi S AD - Faculty of Pharmacy, Mansoura University, Mansoura, Ad-Dakahlia, 35511, Egypt. FAU - Moneim, Mohamed A AU - Moneim MA AD - Faculty of Pharmacy, Alexandria University, 1 El-Khartoum Square - Azarita, Alexandria, 21521, Egypt; Faculty of Pharmacy, Egypt-Japan University of Science and Technology (E-JUST), New Borg El-Arab City, Alexandria, 21934, Egypt. FAU - Al-Wdan, Omnia A AU - Al-Wdan OA AD - Faculty of Pharmacy, Egypt-Japan University of Science and Technology (E-JUST), New Borg El-Arab City, Alexandria, 21934, Egypt. FAU - Nasser, Ahmed A AU - Nasser AA AD - Department of Pharmaceutical Organic Chemistry, Faculty of Pharmacy, Al-Azhar University, Cairo, 11884, Egypt. FAU - Marei, Hassan AU - Marei H AD - Pharmacy Department, Cairo University Student Hospital, Giza, 12613, Egypt. FAU - Ahmed, Esraa AU - Ahmed E AD - Laser Institute for Research and Applications LIRA, Beni-Suef University, Beni-Suef, 62511, Egypt. FAU - Seleem, Mohamed A AU - Seleem MA AD - Department of Pharmaceutical Organic Chemistry, Faculty of Pharmacy, Al-Azhar University, Cairo, 11884, Egypt; Biomedical Sciences Program, University of Science and Technology, Zewail City of Science and Technology, Giza, 12578, Egypt; Center of Certified Reference Materials, Zewail City of Science and Technology, Giza, 12578, Egypt. Electronic address: m.seleem@azhar.edu.eg. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - France TA - Eur J Med Chem JT - European journal of medicinal chemistry JID - 0420510 SB - IM OTO - NOTNLM OT - Anthracimycin OT - C-H functionalization OT - Geldanamycin OT - Late-stage functionalization OT - Ring distortion OT - Semisynthesis COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:10 PHST- 2026/08/10 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:10 [entrez] AID - S0223-5234(26)00916-5 [pii] AID - 10.1016/j.ejmech.2026.119471 [doi] PST - aheadofprint SO - Eur J Med Chem. 2026 Oct 27;321(Pt 3):119471. doi: 10.1016/j.ejmech.2026.119471. PMID- 42956667 OWN - NLM STAT- Publisher LR - 20261029 IS - 1768-3254 (Electronic) IS - 0223-5234 (Linking) VI - 321 IP - Pt 3 DP - 2026 Oct 22 TI - Design, synthesis, and antitumor evaluation of novel VEGFR-2/BRD4 dual inhibitors with potent in vivo efficacy. PG - 119457 LID - S0223-5234(26)00902-5 [pii] LID - 10.1016/j.ejmech.2026.119457 [doi] AB - Aberrant angiogenesis and dysregulated epigenetic transcription are hallmarks of cancer that mutually reinforce tumor progression and therapeutic resistance. Vascular endothelial growth factor receptor-2 (VEGFR-2) is the principal driver of tumor angiogenesis, while bromodomain-containing protein 4 (BRD4) acts as a master transcriptional coactivator of oncogenes. Herein, we report the design, synthesis, and biological evaluation of a series of novel VEGFR-2/BRD4 dual-targeted inhibitors via a pharmacophore hybridization strategy that merges the aryl urea scaffold of sorafenib with the tetrahydropteridinone core of BI-2536. Among the 43 synthesized compounds, 15h emerged as the most potent dual inhibitor. In cellular assays, 15h demonstrated broad-spectrum antiproliferative activity against Colo829, D121, NCI-H446, and MCF-7 cell lines. Notably, 15h substantially outperformed the physical combination of sorafenib plus (+)-JQ-1, achieving 13.7-fold and 9.8-fold greater potency in D121 and NCI-H446 cells, respectively, thereby validating the superiority of a single-molecule dual-targeting strategy over combination therapy. Kinase selectivity profiling revealed that 15h maintains a favorable selectivity window, with VEGFR-2 being the most potently inhibited target. Importantly, in an NCI-H446 xenograft mouse model, 15h administered at 1.5 mg/kg (i.p.) achieved a tumor growth inhibition (TGI) rate of 109.6%, significantly outperforming sorafenib (TGI = 58.9%) and (+)-JQ-1 (TGI = 53.4%), without significant body weight loss during the treatment period. Collectively, these findings establish 15h as a promising VEGFR-2/BRD4 dual-targeted lead compound and validate the molecular hybridization strategy as an effective approach for developing next-generation antitumor agents that overcome the limitations of physical combination regimens. CI - Copyright (c) 2026 Elsevier Masson SAS. All rights reserved. FAU - Liu, Zhenling AU - Liu Z AD - Department of Medicinal Chemistry, Jiangsu Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, Nanjing, 210009, China. FAU - Cui, Zexi AU - Cui Z AD - Department of Medicinal Chemistry, Jiangsu Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, Nanjing, 210009, China. FAU - Zhang, Xiaoling AU - Zhang X AD - Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education, The First Hospital of Jilin University, Changchun, China; National-Local Joint Engineering Laboratory of Animal Models for Human Disease, The First Hospital of Jilin University, Changchun, China. FAU - Wang, Ru AU - Wang R AD - Department of Medicinal Chemistry, Jiangsu Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, Nanjing, 210009, China. Electronic address: ruehlyn8866@163.com. FAU - Dai, Xiangpeng AU - Dai X AD - Key Laboratory of Organ Regeneration and Transplantation of Ministry of Education, The First Hospital of Jilin University, Changchun, China; National-Local Joint Engineering Laboratory of Animal Models for Human Disease, The First Hospital of Jilin University, Changchun, China. Electronic address: daixiangpeng@jlu.edu.cn. FAU - Shi, Lei AU - Shi L AD - Department of Medicinal Chemistry, Jiangsu Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, Nanjing, 210009, China. Electronic address: shilei@cpu.edu.cn. LA - eng PT - Journal Article DEP - 20261022 PL - France TA - Eur J Med Chem JT - European journal of medicinal chemistry JID - 0420510 SB - IM OTO - NOTNLM OT - Antitumor OT - BRD4 OT - Dual inhibitor OT - VEGFR-2 COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:10 PHST- 2026/08/12 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:10 [entrez] AID - S0223-5234(26)00902-5 [pii] AID - 10.1016/j.ejmech.2026.119457 [doi] PST - aheadofprint SO - Eur J Med Chem. 2026 Oct 22;321(Pt 3):119457. doi: 10.1016/j.ejmech.2026.119457. PMID- 42956666 OWN - NLM STAT- Publisher LR - 20261029 IS - 1768-3254 (Electronic) IS - 0223-5234 (Linking) VI - 321 IP - Pt 3 DP - 2026 Oct 27 TI - Discovery and pharmacological profiling of quinoline- and quinoxaline-based phenoxypropanoic acid derivatives as multitarget modulators of PPARalpha, FXR, and LXRalpha receptors. PG - 119458 LID - S0223-5234(26)00903-7 [pii] LID - 10.1016/j.ejmech.2026.119458 [doi] AB - Fibrates, derivatives of phenoxypropanoic acid, have long been used in the therapy of dyslipidemia, targeting the nuclear receptor Peroxisome proliferator-activated receptor alpha (PPARalpha) in the liver and other organs. To discover novel modulators of metabolism-regulating nuclear receptors, we synthesized and evaluated libraries of novel fibrate derivatives based on 2-(4-(quinolin-2-yloxy)phenoxy)propanoic and 2-(4-(quinoxalin-2-yloxy)phenoxy)propanoic acid. We found that some of the prepared compounds exhibit unique properties activating PPARalpha and the Farnesoid X receptor (FXR) while simultaneously inhibiting Liver X receptor alpha (LXRalpha) in cellular in vitro assays. Pilot experiments demonstrate that quinoline derivatives 6d and 6e have suitable pharmacokinetic properties and a significant metabolic effect on selected lipid metabolism genes in vivo in mice and human hepatocyte spheroids. Peroral administration of compound 6d to mice fed a high-fat diet decreased the levels of plasma triglycerides and promoted the expression of critical genes of beta-oxidation in the liver. We thus propose that we have discovered and pharmacologically profiled new quinoline/quinoxaline phenoxypropanoic acid compounds with combined PPARalpha/FXR activation and LXRalpha antagonistic activity, supported by preliminary hepatic target-engagement and in vivo metabolic data. In particular, halogen-substituted 2-(4-(quinolin-2-yloxy)phenoxy)propanoic acid derivatives could be promising multitarget ligands for orchestrating distinct metabolic programs of individual nuclear receptors, supporting their further optimization. CI - Copyright (c) 2026 The Authors. Published by Elsevier Masson SAS.. All rights reserved. FAU - Melnikova, Iuliia AU - Melnikova I AD - Department of Organic and Bioorganic Chemistry, Charles University, Faculty of Pharmacy in Hradec Kralove, Zborovska 2207, 50003, Hradec Kralove, Czech Republic. FAU - Karabanovich, Galina AU - Karabanovich G AD - Department of Organic and Bioorganic Chemistry, Charles University, Faculty of Pharmacy in Hradec Kralove, Zborovska 2207, 50003, Hradec Kralove, Czech Republic. FAU - Dusek, Jan AU - Dusek J AD - Department of Pharmacology and Toxicology, Charles University, Faculty of Pharmacy in Hradec Kralove, Zborovska 2207, 50003, Hradec Kralove, Czech Republic. FAU - Ambroz, Martin AU - Ambroz M AD - Department of Pharmacology and Toxicology, Charles University, Faculty of Pharmacy in Hradec Kralove, Zborovska 2207, 50003, Hradec Kralove, Czech Republic. FAU - Lastuvkova, Hana AU - Lastuvkova H AD - Department of Pharmacology, Charles University, Faculty of Medicine in Hradec Kralove, Zborovska 2207, 50003, Hradec Kralove, Czech Republic. FAU - Smutny, Tomas AU - Smutny T AD - Department of Pharmacology and Toxicology, Charles University, Faculty of Pharmacy in Hradec Kralove, Zborovska 2207, 50003, Hradec Kralove, Czech Republic. FAU - Bajnokova, Maria AU - Bajnokova M AD - Department of Pharmacology and Toxicology, Charles University, Faculty of Pharmacy in Hradec Kralove, Zborovska 2207, 50003, Hradec Kralove, Czech Republic. FAU - Kamaraj, Rajamanikkam AU - Kamaraj R AD - Department of Pharmacology and Toxicology, Charles University, Faculty of Pharmacy in Hradec Kralove, Zborovska 2207, 50003, Hradec Kralove, Czech Republic. FAU - Salam, Rudy AU - Salam R AD - Department of Pharmacy, Faculty of Medicine, Universitas Brawijaya, Jl. Veteran, Malang, 65145, East Java, Indonesia. FAU - Reguli, Adam AU - Reguli A AD - Department of Pharmaceutical Chemistry and Pharmaceutical Analysis, Charles University, Faculty of Pharmacy in Hradec Kralove, Zborovska 2207, 50003, Hradec Kralove, Czech Republic. FAU - Sterbova-Kovarikova, Petra AU - Sterbova-Kovarikova P AD - Department of Pharmaceutical Chemistry and Pharmaceutical Analysis, Charles University, Faculty of Pharmacy in Hradec Kralove, Zborovska 2207, 50003, Hradec Kralove, Czech Republic. FAU - Kucera, Otto AU - Kucera O AD - Department of Physiology, Charles University, Faculty of Medicine in Hradec Kralove, Zborovska 2207, 50003, Hradec Kralove, Czech Republic. FAU - Micuda, Stanislav AU - Micuda S AD - Department of Pharmacology, Charles University, Faculty of Medicine in Hradec Kralove, Zborovska 2207, 50003, Hradec Kralove, Czech Republic. FAU - Roh, Jaroslav AU - Roh J AD - Department of Organic and Bioorganic Chemistry, Charles University, Faculty of Pharmacy in Hradec Kralove, Zborovska 2207, 50003, Hradec Kralove, Czech Republic. Electronic address: rohj@faf.cuni.cz. FAU - Pavek, Petr AU - Pavek P AD - Department of Pharmacology and Toxicology, Charles University, Faculty of Pharmacy in Hradec Kralove, Zborovska 2207, 50003, Hradec Kralove, Czech Republic. Electronic address: pavek@faf.cuni.cz. LA - eng PT - Journal Article DEP - 20261027 PL - France TA - Eur J Med Chem JT - European journal of medicinal chemistry JID - 0420510 SB - IM OTO - NOTNLM OT - Fibrates OT - Ligands OT - Liver OT - Metabolic disease OT - Metabolism OT - Nuclear receptors COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:10 PHST- 2026/07/30 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:10 [entrez] AID - S0223-5234(26)00903-7 [pii] AID - 10.1016/j.ejmech.2026.119458 [doi] PST - aheadofprint SO - Eur J Med Chem. 2026 Oct 27;321(Pt 3):119458. doi: 10.1016/j.ejmech.2026.119458. PMID- 42956664 OWN - NLM STAT- Publisher LR - 20261029 IS - 1768-3254 (Electronic) IS - 0223-5234 (Linking) VI - 321 IP - Pt 3 DP - 2026 Oct 10 TI - Design, synthesis and biological evaluation of pleiotropic prodrugs activated by cholinesterases and releasing a 5-HT(4)R agonist, with in vivo antiamnesic activity. PG - 119383 LID - S0223-5234(26)00828-7 [pii] LID - 10.1016/j.ejmech.2026.119383 [doi] AB - Acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) are hydrolases that specialize in the cleavage of esters such as acetylcholine. However, they can also cleave the carbamate group of certain drugs, such as rivastigmine. This leads to inhibition of these ChEs and preservation of acetylcholine (ACh), their physiological substrate, providing symptomatic benefits in Alzheimer's disease (AD). The present work aims to exploit ChE's capacity to cleave synthetic carbamates, not only to inhibit these enzymes but also to release a potent 5-HT(4) receptor partial agonist upon interaction, thereby exerting an additional disease-modifying effect in AD. Such carbamates would behave as pleiotropic prodrugs. This work relates to the synthesis of the latter compounds and the kinetic study of their enzymatic inhibition to select one of the best candidates from the synthesized compounds for a preliminary in vivo study in an AD model. The latter has been achieved by administering the drug intranasally to bypass the blood-brain barrier (BBB) and avoid peripheral side effects. This has required a special formulation, which was created using liposomes embedded in a thermosensitive mucoadhesive gel. When administered intranasally, the pleiotropic prodrug demonstrated a clear antiamnesic effect in vivo in mice, providing proof of concept for further investigations. CI - Copyright (c) 2026 The Authors. Published by Elsevier Masson SAS.. All rights reserved. FAU - Wang, Alice AU - Wang A AD - Universite Caen Normandie, CERMN UR4258, Normandie Univ, Caen, F-14000, France. FAU - Lahogue, Caroline AU - Lahogue C AD - Universite Caen Normandie, INSERM, Normandie Univ, COMETE UMR-S1075, CHU Caen, Cyceron, Caen, F-14000, France. FAU - Curel, Thomas AU - Curel T AD - IGF, Univ. Montpellier, CNRS, INSERM, Montpellier, F-34094, France. FAU - Lanthier, Caroline AU - Lanthier C AD - Universite Caen Normandie, CERMN UR4258, Normandie Univ, Caen, F-14000, France. FAU - Groo, Anne-Claire AU - Groo AC AD - Universite Caen Normandie, CERMN UR4258, Normandie Univ, Caen, F-14000, France. FAU - Davis, Audrey AU - Davis A AD - Universite Caen Normandie, CERMN UR4258, Normandie Univ, Caen, F-14000, France. FAU - Corvaisier, Sophie AU - Corvaisier S AD - Universite Caen Normandie, CERMN UR4258, Normandie Univ, Caen, F-14000, France; Universite Caen Normandie, INSERM, Normandie Univ, COMETE UMR-S1075, CHU Caen, Cyceron, Caen, F-14000, France. FAU - Fayolle, Dimitri AU - Fayolle D AD - Universite Caen Normandie, CERMN UR4258, Normandie Univ, Caen, F-14000, France. FAU - Modeste, Fabrice AU - Modeste F AD - Institut de Recherche Biomedicale des Armees, Departement des Plateformes et Recherches Technologiques, Unite Developpements Analytiques et Bioanalyse, Bretigny-sur-Orge, F-91220, France. FAU - Brazzolotto, Xavier AU - Brazzolotto X AD - Institut de Recherche Biomedicale des Armees, Departement de Toxicologie et Risques Chimiques, Bretigny-sur-Orge, F-91220, France. FAU - Nachon, Florian AU - Nachon F AD - Institut de Recherche Biomedicale des Armees, Departement de Toxicologie et Risques Chimiques, Bretigny-sur-Orge, F-91220, France. FAU - Lecoutey, Cedric AU - Lecoutey C AD - Universite Caen Normandie, CERMN UR4258, Normandie Univ, Caen, F-14000, France. FAU - Claeysen, Sylvie AU - Claeysen S AD - IGF, Univ. Montpellier, CNRS, INSERM, Montpellier, F-34094, France. FAU - Malzert-Freon, Aurelie AU - Malzert-Freon A AD - Universite Caen Normandie, CERMN UR4258, Normandie Univ, Caen, F-14000, France. FAU - Freret, Thomas AU - Freret T AD - Universite Caen Normandie, INSERM, Normandie Univ, COMETE UMR-S1075, CHU Caen, Cyceron, Caen, F-14000, France. FAU - Boulouard, Michel AU - Boulouard M AD - Universite Caen Normandie, INSERM, Normandie Univ, COMETE UMR-S1075, CHU Caen, Cyceron, Caen, F-14000, France. FAU - Since, Marc AU - Since M AD - Universite Caen Normandie, CERMN UR4258, Normandie Univ, Caen, F-14000, France. FAU - Rochais, Christophe AU - Rochais C AD - Universite Caen Normandie, CERMN UR4258, Normandie Univ, Caen, F-14000, France. Electronic address: christophe.rochais@unicaen.fr. FAU - Dallemagne, Patrick AU - Dallemagne P AD - Universite Caen Normandie, CERMN UR4258, Normandie Univ, Caen, F-14000, France. Electronic address: patrick.dallemagne@unicaen.fr. LA - eng PT - Journal Article DEP - 20261010 PL - France TA - Eur J Med Chem JT - European journal of medicinal chemistry JID - 0420510 SB - IM OTO - NOTNLM OT - 5-HT(4) OT - Alzheimer OT - Cholinesterases OT - Enzyme OT - Intranasal OT - Pleiotropic OT - Prodrug OT - Serotonin COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: P Dallemagne, C Rochais reports a relationship with Ronoma that includes: board membership. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:10 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:10 [entrez] AID - S0223-5234(26)00828-7 [pii] AID - 10.1016/j.ejmech.2026.119383 [doi] PST - aheadofprint SO - Eur J Med Chem. 2026 Oct 10;321(Pt 3):119383. doi: 10.1016/j.ejmech.2026.119383. PMID- 42956661 OWN - NLM STAT- Publisher LR - 20261029 IS - 1095-8673 (Electronic) IS - 0022-4804 (Linking) VI - 328 DP - 2026 Oct 29 TI - Growth of Benign Thyroid Nodules Is Associated With a High Reintervention Rate Without Clear Benefit. PG - 278-283 LID - S0022-4804(26)00631-1 [pii] LID - 10.1016/j.jss.2026.09.040 [doi] AB - INTRODUCTION: The American Thyroid Association (ATA) guidelines recommend routine follow-up ultrasounds following benign thyroid biopsies and repeat fine needle aspiration (FNA) biopsy for nodules that grow. We hypothesized that this may be associated with frequent reintervention without detecting additional cancers. METHODS: A single-center retrospective study of patients with an initial benign thyroid FNA from 2017 to 2021 was performed, 12 mo of data were randomly selected for review. The primary outcome of interest was the rate of repeat FNA or surgery (reintervention), and the secondary outcome of interest was the number of subsequent surveillance ultrasounds. Multivariable regression was used to identify preditors of reintervention. RESULTS: A total of 411 patients with 562 biopsy-proven benign nodules were included. In total, 70 patients (17.1%) underwent at least one repeat biopsy, and 15 patients (3.9%) underwent thyroid surgery. Two patients (0.5%) were ultimately diagnosed with thyroid cancer, neither in the biopsied nodule. During a median follow-up period of 26.4 mo, 282 (68.6%) had at least one follow-up ultrasound, while 64 (15.6%) underwent five or more follow-up ultrasounds. At 5 y, nearly one-third of patients still under surveillance had undergone either repeat FNA or surgery. Patients with nodules meeting ATA growth criteria for repeat FNA were more likely to undergo repeat biopsy than those whose nodules did not (48.5% versus 23.5%, P = 0.02). CONCLUSIONS: Malignancy after an initial benign thyroid FNA biopsy is rare, and subsequent surveillance is resource-intensive without clear benefit. The growth criteria for repeat FNA in the 2015 ATA guidelines were associated with reintervention without detection of malignancies. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Koul, Akash AU - Koul A AD - Department of Surgery, Albany Medical College, Albany, New York. FAU - Wang, Dereck AU - Wang D AD - Department of Surgery, Albany Medical College, Albany, New York. FAU - Tandon, Mihir AU - Tandon M AD - Department of Surgery, Albany Medical College, Albany, New York. FAU - Chao, Joshua C AU - Chao JC AD - Department of Surgery, Albany Medical College, Albany, New York. FAU - Thiesmeyer, Jessica W AU - Thiesmeyer JW AD - Department of Surgery, Albany Medical College, Albany, New York. FAU - Farhat, Nada A AU - Farhat NA AD - Department of Pathology, Albany Medical College, Albany, New York. FAU - Ullmann, Timothy M AU - Ullmann TM AD - Department of Surgery, Albany Medical College, Albany, New York. Electronic address: ullmant@amc.edu. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Surg Res JT - The Journal of surgical research JID - 0376340 SB - IM OTO - NOTNLM OT - Benign thyroid nodules OT - Fine needle aspiration OT - Overtreatment OT - Surveillance EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:10 PHST- 2026/01/14 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:10 [entrez] AID - S0022-4804(26)00631-1 [pii] AID - 10.1016/j.jss.2026.09.040 [doi] PST - aheadofprint SO - J Surg Res. 2026 Oct 29;328:278-283. doi: 10.1016/j.jss.2026.09.040. PMID- 42956658 OWN - NLM STAT- Publisher LR - 20261029 IS - 1872-7123 (Electronic) IS - 0165-1781 (Linking) VI - 367 DP - 2026 Oct 23 TI - Beyond tics: executive functioning and psychopathological comorbidities in children and adolescents with Tourette syndrome. A systematic review. PG - 117551 LID - S0165-1781(26)00610-4 [pii] LID - 10.1016/j.psychres.2026.117551 [doi] AB - Pediatric Tourette syndrome (TS) is heterogeneous, and functional outcomes are associated not only with tic severity but also with comorbid psychopathology and self-regulatory difficulties. We systematically reviewed (i) psychiatric/psychosocial comorbidities and their links to functioning and (ii) executive functioning (EF), explicitly contrasting performance-based and ecological measures. The protocol was preregistered on OSF and reported according to PRISMA. PubMed, PsycINFO, and Web of Science were searched from inception to 30 March 2025. Eligible studies included participants 0.07), consistent with feature quality and the broader retraining scope of EP3 outweighing aggregation strategy for cross-domain transfer. Interpretability analysis shows both architectures enrich for tumor and inflammation, with the top-attended regions of CA spanning 66% of the biopsy area versus 29% for NCA; both progressively converge on the most challenging task. CONCLUSION: Context-aware models significantly outperform attention-based aggregation within-domain, but this advantage does not persist uniformly under domain shift. Cross-domain transfer depends on the task, on feature quality together with retraining scope, and is associated with the structural domain shift between core-needle biopsies and surgical resections, rather than being uniform. These findings provide practical guidance for architecture-feature selection across deployment scenarios. CI - Copyright (c) 2026 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Fernandez-Martin, Claudio AU - Fernandez-Martin C AD - Instituto Universitario de Investigacion en Tecnologia Centrada en el Ser Humano, Universitat Politecnica de Valencia, Valencia, Spain. Electronic address: clferma1@i3b.upv.es. FAU - Kiraz, Umay AU - Kiraz U AD - Department of Chemistry, Bioscience and Environmental Engineering, University of Stavanger, Stavanger, Norway; Department of Pathology, Stavanger University Hospital, Stavanger, Norway. FAU - Janssen, Emiel A M AU - Janssen EAM AD - Department of Chemistry, Bioscience and Environmental Engineering, University of Stavanger, Stavanger, Norway; Department of Pathology, Stavanger University Hospital, Stavanger, Norway. FAU - Naranjo, Valery AU - Naranjo V AD - Instituto Universitario de Investigacion en Tecnologia Centrada en el Ser Humano, Universitat Politecnica de Valencia, Valencia, Spain. FAU - Silva-Rodriguez, Julio AU - Silva-Rodriguez J AD - Computer Vision Lab, ETH Zurich, Zurich, Switzerland. FAU - Morales, Sandra AU - Morales S AD - Instituto Universitario de Investigacion en Tecnologia Centrada en el Ser Humano, Universitat Politecnica de Valencia, Valencia, Spain. LA - eng PT - Journal Article DEP - 20261023 PL - Ireland TA - Comput Methods Programs Biomed JT - Computer methods and programs in biomedicine JID - 8506513 SB - IM OTO - NOTNLM OT - Breast cancer OT - Computational pathology OT - Context-aware multiple-instance learning OT - Graph neural networks OT - Molecular subtype prediction OT - Weakly supervised learning COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Claudio Fernandez-Martin reports financial support was provided by European Union. Umay Kiraz reports financial support was provided by European Union. Sandra Morales reports was provided by Generalitat of Valencia. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:09 PHST- 2025/11/12 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:09 [entrez] AID - S0169-2607(26)00445-1 [pii] AID - 10.1016/j.cmpb.2026.109696 [doi] PST - aheadofprint SO - Comput Methods Programs Biomed. 2026 Oct 23;288:109696. doi: 10.1016/j.cmpb.2026.109696. PMID- 42956627 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-0070 (Electronic) IS - 0732-8893 (Linking) VI - 117 IP - 1 DP - 2026 Oct 26 TI - First-void urine testing for high-risk human papillomavirus: A within-cohort comparison of three preanalytical approaches. PG - 117712 LID - S0732-8893(26)00463-3 [pii] LID - 10.1016/j.diagmicrobio.2026.117712 [doi] AB - OBJECTIVES: To compare three preanalytical approaches for first-void urine (FVU)-based high-risk human papillomavirus (hrHPV) DNA detection against paired cervical swab (CS) specimens. METHODS: This cross-sectional observational study included 69 participants with a previous positive hrHPV screening result and paired CS and FVU specimens. From each FVU specimen, three urine preparations were generated: unpreserved urine, EDTA-treated urine for DNA stabilization, urine buffered with urine conservation medium (UCM; Novosanis, Wijnegem, Belgium). All specimens were analyzed using the QIAscreen HPV PCR Test (Qiagen, Hilden, Germany). Agreement with paired CS specimens, genotype-specific agreement, paired CS cycle threshold (Ct) values, and participant-reported perceptions of urine sampling were assessed. RESULTS: Overall agreement between urine and CS specimens was moderate across all three approaches, with Cohen's kappa values of 0.566 for unpreserved urine, 0.538 for EDTA-treated urine, and 0.581 for UCM-buffered urine. Paired CS Ct values were lower in participants with hrHPV DNA-positive urine specimens than in those with hrHPV DNA-negative urine specimens, consistent with greater urinary detectability at higher cervical hrHPV DNA levels. Genotype-specific agreement was stronger for HPV16 and HPV18 than for pooled non-16/18 hrHPV. Participant-reported data suggested good acceptability of urine sampling. CONCLUSIONS: The three approaches showed broadly similar results to one another, while agreement with paired CS specimens was moderate across all approaches. These findings support further evaluation of urine-based hrHPV testing as a non-invasive sampling option that could help expand access to testing, particularly in low-income and other resource-limited settings. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Menekse Yurt, Sumeyra Ebru AU - Menekse Yurt SE AD - Department of Medical Microbiology, Faculty of Medicine, Erciyes University, Kayseri, Turkey. FAU - Parkan, Omur Mustafa AU - Parkan OM AD - Department of Medical Microbiology, Faculty of Medicine, Erciyes University, Kayseri, Turkey. Electronic address: omurparkan@erciyes.edu.tr. FAU - Sahin, Seda AU - Sahin S AD - Department of Obstetrics and Gynecology, Memorial Hospital, Kayseri, Turkey. FAU - Yuksek, Sahin AU - Yuksek S AD - Department of Obstetrics and Gynecology, Kayseri City Hospital, Kayseri, Turkey. FAU - Sen, Ertugrul AU - Sen E AD - Department of Obstetrics and Gynecology, Kayseri City Hospital, Kayseri, Turkey. FAU - Gokahmetoglu, Selma AU - Gokahmetoglu S AD - Department of Medical Microbiology, Faculty of Medicine, Erciyes University, Kayseri, Turkey. LA - eng PT - Journal Article DEP - 20261026 PL - United States TA - Diagn Microbiol Infect Dis JT - Diagnostic microbiology and infectious disease JID - 8305899 SB - IM OTO - NOTNLM OT - Cervical swab OT - First-void urine OT - High-risk human papillomavirus OT - Preanalytical processing OT - Real-time PCR OT - Self-sampling OT - Urine-based HPV testing COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:09 PHST- 2026/04/29 00:00 [received] PHST- 2026/09/04 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:09 [entrez] AID - S0732-8893(26)00463-3 [pii] AID - 10.1016/j.diagmicrobio.2026.117712 [doi] PST - aheadofprint SO - Diagn Microbiol Infect Dis. 2026 Oct 26;117(1):117712. doi: 10.1016/j.diagmicrobio.2026.117712. PMID- 42956626 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-0070 (Electronic) IS - 0732-8893 (Linking) VI - 117 IP - 1 DP - 2026 Oct 23 TI - Chikungunya virus - associated meningitis with transient encephalopathy: a case report. PG - 117700 LID - S0732-8893(26)00451-7 [pii] LID - 10.1016/j.diagmicrobio.2026.117700 [doi] AB - BACKGROUND: Chikungunya virus (CHIKV) typically causes acute fever, arthralgia, and rash; central nervous system involvement is rare, and early diagnosis is challenging due to nonspecific presentations. CASE PRESENTATION: We report a case of a 37-year-old previously healthy male who presented with fever, confusion, and syncope during a local chikungunya fever outbreak in his city. Neurological examination revealed positive meningeal signs, and his Glasgow Coma Scale score was 14. Targeted next-generation sequencing (tNGS) detected chikungunya virus (CHIKV) in both blood and cerebrospinal fluid. Following supportive care, the patient made a full recovery and remained well at the six-month follow-up, with a favorable prognosis. CONCLUSIONS: In our case, tNGS detected CSF CHIKV RNA after negative mNGS, suggesting possible added value of targeted enrichment in selected settings that requires validation in larger studies. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Wu, Juan AU - Wu J AD - Department of Laboratory Medicine, Jieyang People's Hospital, Jieyang, Guangdong, China. FAU - He, Jieting AU - He J AD - Department of Pathology, Jieyang People's Hospital, Jieyang, Guangdong, China. FAU - Lin, Huijin AU - Lin H AD - Department of Respiratory and Critical Care Medicine, Jieyang People's Hospital, Jieyang, Guangdong, China. FAU - Hou, Guisong AU - Hou G AD - Medical Imaging Center, Jieyang People's Hospital, Jieyang, Guangdong, China. FAU - Huang, Xudong AU - Huang X AD - Department of Laboratory Medicine, Jieyang People's Hospital, Jieyang, Guangdong, China. FAU - Chen, Peisong AU - Chen P AD - Department of Laboratory Medicine, People's Hospital of Boluo County, Huizhou, Guangdong, China; Department of Laboratory Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China. Electronic address: chps@mail3.sysu.edu.cn. LA - eng PT - Case Reports PT - Journal Article DEP - 20261023 PL - United States TA - Diagn Microbiol Infect Dis JT - Diagnostic microbiology and infectious disease JID - 8305899 SB - IM OTO - NOTNLM OT - Chikungunya virus OT - Molecular diagnostics OT - Viral meningoencephalitis OT - tNGS COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Peisong Chen reports administrative support, article publishing charges, and writing assistance were provided by Noncommunicable Chronic Diseases-National Science and Technology Major Project. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:09 PHST- 2026/06/30 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:09 [entrez] AID - S0732-8893(26)00451-7 [pii] AID - 10.1016/j.diagmicrobio.2026.117700 [doi] PST - aheadofprint SO - Diagn Microbiol Infect Dis. 2026 Oct 23;117(1):117700. doi: 10.1016/j.diagmicrobio.2026.117700. PMID- 42956620 OWN - NLM STAT- Publisher LR - 20261029 IS - 1095-8630 (Electronic) IS - 0301-4797 (Linking) VI - 419 DP - 2026 Oct 29 TI - How to manage meadows effectively? Flagship, keystone & diagnostic species perspective. PG - 131203 LID - S0301-4797(26)02663-0 [pii] LID - 10.1016/j.jenvman.2026.131203 [doi] AB - We investigated the long-term effects of meadow management regimes (abandonment vs. mowing, and its frequency), plant invasion, and habitat availability on three different meadow ecosystem components. The system consists of (1) native plant Sanguisorba officinalis, (2) ant assemblages, and (3) butterfly species of the genus Phengaris, known as flagships for biodiversity conservation. The investigated wet meadow communities responded quite similarly to management regimes. In general, declining biodiversity was related to either complete abandonment or more intensive management (with mowing more than once per year). The focal native plant cover was affected by management and invasive plants. Mowing frequency did not affect ant diversity or population fluctuations of Phengaris butterflies over a five-year period. Low frequency mowing (one cut per year) is crucial to long-term persistence of the studied species, and S. officinalis cover is a good indicator for assessing habitat condition of studied meadows. The establishment of non-intensive management and occasionally more intensive mowing for active control of invasive plants is needed to preserve the investigated wet meadows of high conservation value and complex system of organisms inhabiting them. CI - Copyright (c) 2026 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Kajzer-Bonk, Joanna AU - Kajzer-Bonk J AD - Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University, Gronostajowa 9, Krakow, 30-387, Poland. Electronic address: joanna.kajzer@uj.edu.pl. FAU - Nowicki, Piotr AU - Nowicki P AD - Institute of Environmental Sciences, Faculty of Biology, Jagiellonian University, Gronostajowa 7, Krakow, 30-387, Poland. Electronic address: piotr.nowicki@uj.edu.pl. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - J Environ Manage JT - Journal of environmental management JID - 0401664 SB - IM OTO - NOTNLM OT - Biodiversity loss OT - Invasive plants OT - Land-use change OT - Maculinea OT - Mowing frequency OT - Wet meadows COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:09 PHST- 2026/01/14 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:09 [entrez] AID - S0301-4797(26)02663-0 [pii] AID - 10.1016/j.jenvman.2026.131203 [doi] PST - aheadofprint SO - J Environ Manage. 2026 Oct 29;419:131203. doi: 10.1016/j.jenvman.2026.131203. PMID- 42956612 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-3573 (Electronic) IS - 0039-9140 (Linking) VI - 314 DP - 2026 Oct 26 TI - Nano-Al(2)O(3)-doped PVA gel polymer electrolytes: A promising route for efficient VRLA battery applications. PG - 130837 LID - S0039-9140(26)01493-1 [pii] LID - 10.1016/j.talanta.2026.130837 [doi] AB - Aluminium oxide (Al(2)O(3)) nanoparticles have proven to be extremely promising additive materials for future energy storage due to their good electrochemical and conductance properties. Herein, an Al(2)O(3)-doped Poly(vinyl alcohol) (PVA) gel electrolyte containing 1, 3, 6, and 9 wt% Al(2)O(3) relative to the PVA content, with pure PVA, was prepared and systematically evaluated for application in valve-regulated lead-acid (VRLA) batteries. Among the investigated compositions, the 6 wt% Al(2)O(3)@PVA gel exhibited the most favourable combination of ionic conductivity and electrochemical performance and was therefore selected for prototype battery evaluation. The characterisation included Fourier transform infrared spectroscopy for the structure of the gel, measurements of ionic conductivity, and a water retention study. Electrochemical techniques like cyclic voltammetry, electrochemical impedance spectroscopy, potentiodynamic polarisation, and galvanostatic charge-discharge were conducted simultaneously to assess how much the ideal loading of nanoscale particles. The first sample of VRLA battery with the optimised gel electrolyte gave the highest discharge capacity of 4.08 mAh at a loading current of 20 mA and at 0.32 Wh kg(-1), a considerable energy density and a power density of 3.2 W kg(-1) as shown in the Ragone plot. The cell was able to maintain about 100% coulombic efficiency, which is quite remarkable for rechargeable batteries. However, the cell's discharge capacity after 500 cycles decreased by nearly 12%, meaning that it still had a strong performance after a number of cycles. Through detailed investigation, these superior features result mainly from the combined effects of Al(2)O(3) and PVA, which enhance ion mobility, mechanical resistance, and the effectiveness of electrical transport to charge. Therefore, this development opens up new applications for the lead-acid battery in different domains like renewable energy systems, electric vehicles, and portable devices. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Chikkatti, Bipin S AU - Chikkatti BS AD - Department of Chemistry, KLE Technological University, Hubballi, 580031, India. FAU - Sajjan, Ashok M AU - Sajjan AM AD - Department of Chemistry, KLE Technological University, Hubballi, 580031, India; Centre of Excellence in Materials Science, School of Mechanical Engineering, KLE Technological University, Hubballi, 580031, India. Electronic address: am_sajjan@kletech.ac.in. FAU - Banapurmath, Nagaraj R AU - Banapurmath NR AD - Centre of Excellence in Materials Science, School of Mechanical Engineering, KLE Technological University, Hubballi, 580031, India. FAU - Mousa, Saeed AU - Mousa S AD - Department of Mechanical Engineering, College of Engineering and Computer Science, Jazan University, 706, Jazan, Saudi Arabia. FAU - Yunus Khan, T M AU - Yunus Khan TM AD - Department of Mechanical Engineering, College of Engineering, King Khalid University, Abha, 61421, Saudi Arabia. FAU - Umarfarooq, M A AU - Umarfarooq MA AD - Centre for Materials Science, Department of Mechanical Engineering, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, 641021, India. FAU - Choudhari, Santosh K AU - Choudhari SK AD - School of Basic and Applied Sciences, Dayanada Sagar University, Kudlu Gate Campus, Bangaluru, 560068, India. FAU - Tadasad, Manasa S AU - Tadasad MS AD - Department of Electrical & Electronics Engineering, KLE Technological University, Hubballi, 580031, India. LA - eng PT - Journal Article DEP - 20261026 PL - Netherlands TA - Talanta JT - Talanta JID - 2984816R SB - IM OTO - NOTNLM OT - Al(2)O(3) OT - Discharge capacity OT - Gel polymer electrolyte OT - PVA OT - VRLA battery COIS- Declaration of competing interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:08 PHST- 2026/08/03 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:08 [entrez] AID - S0039-9140(26)01493-1 [pii] AID - 10.1016/j.talanta.2026.130837 [doi] PST - aheadofprint SO - Talanta. 2026 Oct 26;314:130837. doi: 10.1016/j.talanta.2026.130837. PMID- 42956611 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-3573 (Electronic) IS - 0039-9140 (Linking) VI - 314 DP - 2026 Oct 29 TI - Label-free fluorescent detection of NAD(+) and ATP by coupling enzymatic DNA reactions with an intramolecular split G-quadruplex. PG - 130853 LID - S0039-9140(26)01509-2 [pii] LID - 10.1016/j.talanta.2026.130853 [doi] AB - Nicotinamide adenine dinucleotide (NAD(+)) and adenosine triphosphate (ATP) are crucial small molecules linked to diverse physiological processes and diseases, making their robust quantification of paramount importance. Herein, we propose a novel label-free fluorescence sensing platform for the quantitative detection of NAD(+) and ATP, by seamlessly coupling an intramolecular split G-quadruplex (Intra-SG)-based DNA probe with enzymatic DNA reactions, including high-fidelity cofactor recognition by DNA ligases and the structure-dependent cleavage by Exonuclease III (Exo III). Exo III effectively digests the nicked duplex to release a trigger strand (TS), which subsequently hybridizes with the spacer of the Intra-SG, and triggers the complexation between the Intra-SG and fluorescent dyes (Thioflavin T, ThT) and subsequent fluorescent enhancement. Target-induced ligation yields a repaired duplex that resists Exo III cleavage, resulting in fluorescence quenching. This strategy exhibited linear ranges of 25-300 nM for NAD(+) and 50-300 nM for ATP, with detection limits of 12.35 nM and 29.46 nM, respectively. Furthermore, it guarantees exquisite selectivity against structural analogues, and practical applicability in complex biological matrices. Overall, the effectiveness of the Intra-SG-based single probe is validated, and it is anticipated to be integrated with diverse DNA reactions for multifarious bioanalysis. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Lin, Lu AU - Lin L AD - State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China; School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, China. FAU - Yin, Fang AU - Yin F AD - State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China. FAU - Ren, Jiangtao AU - Ren J AD - College of Science, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address: renjiangtao@njau.edu.cn. FAU - Wang, Jin AU - Wang J AD - State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China; School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, China; Department of Chemistry and Physics, State University of New York at Stony Brook, Stony Brook, NY, 11794-3400, United States. Electronic address: jin.wang.1@stonybrook.edu. FAU - Wang, Erkang AU - Wang E AD - State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China; School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, 230026, China. LA - eng PT - Journal Article DEP - 20261029 PL - Netherlands TA - Talanta JT - Talanta JID - 2984816R SB - IM OTO - NOTNLM OT - ATP OT - DNA ligation OT - Exonuclease III OT - Intramolecular split G-quadruplex OT - Label-free OT - NAD(+) COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:08 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:08 [entrez] AID - S0039-9140(26)01509-2 [pii] AID - 10.1016/j.talanta.2026.130853 [doi] PST - aheadofprint SO - Talanta. 2026 Oct 29;314:130853. doi: 10.1016/j.talanta.2026.130853. PMID- 42956610 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-3573 (Electronic) IS - 0039-9140 (Linking) VI - 314 DP - 2026 Oct 22 TI - Deep learning-assisted portable sensing platform based on a fluorescent MOF-polymer mixed-matrix membrane for rapid detection of nitrazepam in biological samples. PG - 130815 LID - S0039-9140(26)01471-2 [pii] LID - 10.1016/j.talanta.2026.130815 [doi] AB - Nitrazepam (NZP) is a benzodiazepine sedative-hypnotic widely used in the treatment of anxiety and sleep disorders. However, non-medical use of NZP as a new psychoactive substance poses serious health risks. Therefore, rapid and accurate detection of NZP in biological samples is important for point-of-care testing. Herein, a deep learning-assisted fluorescent sensing platform was developed based on a mixed-matrix membrane (1@PMMA), fabricated by incorporating a Cd-MOF, [Cd(L)(H(2)O)(2)].3H(2)O(n) (1) (H(2)L = 5-(3-(pyridin-4-yl)ureido)isophthalic acid), into a poly(methyl methacrylate) (PMMA) matrix for NZP detection. The resulting 1@PMMA exhibited good mechanical stability, processability, and fluorescence stability in biological samples. Upon exposure to NZP, 1@PMMA showed efficient fluorescence quenching, with a quenching efficiency of 96.19%, a detection limit of 0.21 muM, and a response time of approximately 2 s. The membrane maintained high selectivity in the presence of common biological components and stable sensing performance over five quenching-regeneration cycles. A smartphone-based fluorescence sensing method enabled portable visual detection of NZP through extraction of RGB values from fluorescence images of 1@PMMA. Additionally, a deep learning-based photo-to-result platform employing a ResNet-18 model directly predicted NZP concentrations from fluorescence images without manual data processing, showing a strong linear relationship between predicted and experimental concentrations (R(2) = 0.9949). For spiked urine and serum samples, recoveries of 95.18-100.46% were obtained, with relative standard deviations of 0.18-3.10%. This work integrates a fluorescent MOF-based mixed-matrix membrane with a deep learning-assisted photo-to-result strategy for on-site quantitative determination of NZP in biological samples. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Lei, Mingyuan AU - Lei M AD - Characteristic Laboratory of Forensic Science in Universities of Shandong Province, Shandong University of Political Science and Law, Jinan, 250014, China. Electronic address: leimingyuan@sdupsl.edu.cn. FAU - Yang, Kunnan AU - Yang K AD - College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252059, China. FAU - Ma, Junchao AU - Ma J AD - Characteristic Laboratory of Forensic Science in Universities of Shandong Province, Shandong University of Political Science and Law, Jinan, 250014, China. FAU - Zhang, Yanbin AU - Zhang Y AD - School of Intelligent Manufacturing and Elevator, Huzhou Vocational and Technical College, Huzhou, 313099, China. FAU - Lu, Wenhui AU - Lu W AD - Characteristic Laboratory of Forensic Science in Universities of Shandong Province, Shandong University of Political Science and Law, Jinan, 250014, China. FAU - Li, Xuebo AU - Li X AD - Characteristic Laboratory of Forensic Science in Universities of Shandong Province, Shandong University of Political Science and Law, Jinan, 250014, China. FAU - Huo, Sihan AU - Huo S AD - Characteristic Laboratory of Forensic Science in Universities of Shandong Province, Shandong University of Political Science and Law, Jinan, 250014, China. FAU - Pei, Haimeng AU - Pei H AD - Department of Clinical Laboratory, The Second Qilu Hospital of Shandong University, Jinan, 250012, China. FAU - Zhang, Qingfu AU - Zhang Q AD - College of Chemistry and Chemical Engineering, Liaocheng University, Liaocheng, 252059, China. Electronic address: zhangqingfu@lcu.edu.cn. LA - eng PT - Journal Article DEP - 20261022 PL - Netherlands TA - Talanta JT - Talanta JID - 2984816R SB - IM OTO - NOTNLM OT - Deep learning OT - Fluorescence sensing OT - Metal-organic frameworks OT - Mixed-matrix membrane OT - Nitrazepam OT - Smartphone-based detection COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:08 PHST- 2026/06/03 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:08 [entrez] AID - S0039-9140(26)01471-2 [pii] AID - 10.1016/j.talanta.2026.130815 [doi] PST - aheadofprint SO - Talanta. 2026 Oct 22;314:130815. doi: 10.1016/j.talanta.2026.130815. PMID- 42956609 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-3573 (Electronic) IS - 0039-9140 (Linking) VI - 314 DP - 2026 Oct 23 TI - Smartphone-integrated Eu/Tb bimetallic MOF ratiometric fluorescence platform for rapid determination of sodium dehydroacetate in food. PG - 130817 LID - S0039-9140(26)01473-6 [pii] LID - 10.1016/j.talanta.2026.130817 [doi] AB - The development of a sensitive and rapid strategy for quantitative detection of sodium dehydroacetate (SDHA) holds great significance for human health and food safety. Herein, a series of bimetallic Eu/Tb-MOFs with various Eu/Tb molar ratios were fabricated via solvothermal method based on terephthalic acid (H(2)BDC), namely, Eu(x)Tb(1-x)-BDC. The Eu(0.1)Tb(0.9)-BDC was optimized as the best ratiometric fluorescent sensor for on-off sensing of SDHA. The Eu(0.1)Tb(0.9)-BDC exhibits excellent stability and presents ratiometric response toward SDHA with the limit of detection (LOD) as 63.4 nM. The Eu(0.1)Tb(0.9)-BDC also possesses outstanding selectivity and strong anti-interference capability against common coexisting substances. In real flour and noodle sample analysis, good recoveries of 90.87%-106.7% were obtained, with relative standard deviations (RSDs) lower than 4.89%. Moreover, due to the distinct and visible color changes from red to green, the integration of the sensor with a portable smartphone allowed sensitive, on-site and visual detection of SDHA. The sensing mechanism should be combined with the antenna effect of SDHA to Tb(3+), competitive absorption and weak interaction between SDHA and the sensor. This study proposes an efficient strategy for monitoring SDHA and also broadens the application of dual-lanthanide MOFs in food analysis. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Li, Zhong-Lin AU - Li ZL AD - College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi, 830054, China; Key Laboratory of Life-Organic Analysis of Shandong Province, School of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, 273165, China. FAU - Zhao, Fang-Hua AU - Zhao FH AD - Key Laboratory of Life-Organic Analysis of Shandong Province, School of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, 273165, China. FAU - Guan, Ming AU - Guan M AD - College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi, 830054, China. Electronic address: 635880760@qq.com. FAU - You, Jinmao AU - You J AD - Key Laboratory of Life-Organic Analysis of Shandong Province, School of Chemistry and Chemical Engineering, Qufu Normal University, Qufu, 273165, China; College of Chemistry and Chemical Engineering, Shaoxing University, Shaoxing, 312000, China. Electronic address: jmyou6304@163.com. LA - eng PT - Journal Article DEP - 20261023 PL - Netherlands TA - Talanta JT - Talanta JID - 2984816R SB - IM OTO - NOTNLM OT - Bimetallic Eu/Tb-MOFs OT - Ratiometric fluorescent sensor OT - Smartphone-integrated platform OT - Sodium dehydroacetate COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:08 PHST- 2026/08/24 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:08 [entrez] AID - S0039-9140(26)01473-6 [pii] AID - 10.1016/j.talanta.2026.130817 [doi] PST - aheadofprint SO - Talanta. 2026 Oct 23;314:130817. doi: 10.1016/j.talanta.2026.130817. PMID- 42956608 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-3573 (Electronic) IS - 0039-9140 (Linking) VI - 314 DP - 2026 Oct 27 TI - Next-generation analytical platforms for neurochemical sensing: from conventional biosensors to intelligent brain interfaces. PG - 130827 LID - S0039-9140(26)01483-9 [pii] LID - 10.1016/j.talanta.2026.130827 [doi] AB - Neurochemical sensing is central to understanding brain function, neurological disorders, and precision neurotherapeutics. Despite advances in electrochemical, optical, implantable, wearable, and genetically encoded technologies, current platforms remain limited by molecular selectivity, long-term stability, biofouling, complex data interpretation, and clinical translation. This review critically examines 83 representative studies encompassing nanomaterial-enabled electrochemical sensors, implantable and wearable devices, genetically encoded and CRISPR-engineered systems, AI-assisted analytical frameworks, and multimodal bioelectronic interfaces. Among the reviewed platforms, 68.7% were based on electrochemical or electrical sensing, whereas only 9.6% incorporated explicit AI/ML-assisted data analysis, indicating that intelligent analytical functionalities remain relatively limited. AI-assisted platforms have shown promising capabilities for signal discrimination, quantitative analysis, and enhanced analytical performance across diverse neurochemical sensing modalities, but their reported performance remains highly dependent on the sensing architecture, experimental conditions, and level of validation. Rather than providing a conventional technology-centered survey, this review establishes a unified framework for evaluating neurochemical sensing across five dimensions: molecular selectivity, spatiotemporal resolution, operational stability, intelligent data interpretation, and translational readiness. The reviewed evidence indicates a transition from conventional single-modality sensing toward integrated platforms incorporating multimodal detection and intelligent data interpretation, with adaptive and closed-loop interfacing emerging as future directions. By connecting advances in molecular recognition, functional materials, bioelectronics, and AI, this review identifies key design principles, persistent bottlenecks, and realistic priorities for translating next-generation neurochemical sensing platforms into reliable and clinically relevant intelligent interfaces. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Ardah, Mustafa T AU - Ardah MT AD - Faculty of Allied Medical Sciences, Hourani Center for Applied Scientific Research, Al-Ahliyya Amman University, Amman, Jordan. FAU - Kubaev, Aziz AU - Kubaev A AD - Department of Maxillofacial Surgery, Samarkand State Medical University, Amir Temur Street, Samarkand, 140100, Uzbekistan. FAU - Patel, Jalpa R AU - Patel JR AD - Department of Pharmacy, Faculty of Pharmacy, Gokul Global University, Sidhpur, Gujarat, India. FAU - Saxena, Rahul AU - Saxena R AD - Department of Biochemistry, Sharda University, knowledge Park III, Greater Noida, India. FAU - Mutee, Ahmed Faisal AU - Mutee AF AD - College of Pharmacy, Alnoor University, Mosul, Iraq. FAU - Al-Hussein, Maryam Abdul AU - Al-Hussein MA AD - Department of Medical Laboratory Technologies, College of Medical Technologies, The Islamic University, Najaf, Iraq. FAU - Sharma, Chandan AU - Sharma C AD - University Institute of Pharma Sciences, Chandigarh University, Mohali, Punjab, India. FAU - Shayesteh, Omid Heydari AU - Shayesteh OH AD - Department of Medicinal Chemistry, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran. Electronic address: omid.shayesteh777@gmail.com. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Netherlands TA - Talanta JT - Talanta JID - 2984816R SB - IM OTO - NOTNLM OT - Artificial intelligence OT - Biosensors OT - Genetically encoded sensors OT - Neurochemical sensing OT - Neurotransmitters COIS- Declaration of competing interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:08 PHST- 2026/08/23 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:08 [entrez] AID - S0039-9140(26)01483-9 [pii] AID - 10.1016/j.talanta.2026.130827 [doi] PST - aheadofprint SO - Talanta. 2026 Oct 27;314:130827. doi: 10.1016/j.talanta.2026.130827. PMID- 42956607 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-3573 (Electronic) IS - 0039-9140 (Linking) VI - 314 DP - 2026 Oct 26 TI - Dual-zone swelling microneedle-smartphone platform for simultaneous colorimetric quantification of glucose and pH in diabetic wound interstitial fluid. PG - 130836 LID - S0039-9140(26)01492-X [pii] LID - 10.1016/j.talanta.2026.130836 [doi] AB - Diabetic wounds undergo dynamic changes in local metabolism and infection-associated microenvironments, making timely assessment of wound status challenging. Herein, we report, to the best of our knowledge, the first dual-zone swelling microneedle-smartphone platform for simultaneous colorimetric quantification of glucose and pH directly from diabetic wound interstitial fluid (ISF). The platform combines a swelling microneedle patch, termed "IDB-CMN", with a companion WeChat mini program, "IDB-CMN Insight". Two spatially separated response zones are incorporated into the patch, using a glucose oxidase-horseradish peroxidase-3,3',5,5'-tetramethylbenzidine (GOx-HRP-TMB) cascade for glucose detection and m-cresol purple (mCP) for pH sensing. After local ISF uptake, the resulting colorimetric responses are analyzed by IDB-CMN Insight, which extracts RGB values from user-selected regions and converts them into quantitative glucose concentrations and pH values using pre-established calibration equations. The patch exhibited a mechanical strength of approximately 0.4 N per needle and a maximum swelling ratio of approximately 305%, with quantitative response ranges of 0.5-5.0 mg mL(-1) for glucose and pH 6.0-10.0. In non-infected and Staphylococcus aureus-infected diabetic mouse wounds, the platform captured temporal changes in local glucose and pH that were consistent with conventional measurements, while infected wounds maintained a more alkaline microenvironment. Concurrent analysis of these two parameters provides complementary information on local metabolic and infection-associated changes and may offer early indications of adverse microenvironmental shifts during wound healing, supporting future risk assessment and treatment optimization. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Zhou, Yan AU - Zhou Y AD - State Key Laboratory of Chemo and Biosensing, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha, 410082, China. FAU - Li, Siyu AU - Li S AD - School of Artificial Intelligence and Robotics, Hunan University, Changsha, 410082, China; School of Automation and Electrical Engineering, Zhejiang University of Science and Technology, Hangzhou, 310023, China. FAU - He, Lin AU - He L AD - State Key Laboratory of Chemo and Biosensing, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha, 410082, China. FAU - Qi, Lanlin AU - Qi L AD - State Key Laboratory of Chemo and Biosensing, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha, 410082, China. FAU - Yin, Guanyu AU - Yin G AD - State Key Laboratory of Chemo and Biosensing, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha, 410082, China. FAU - Zhou, Huimin AU - Zhou H AD - State Key Laboratory of Chemo and Biosensing, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha, 410082, China. FAU - Long, Wendong AU - Long W AD - State Key Laboratory of Chemo and Biosensing, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha, 410082, China. FAU - Chen, Yangqing AU - Chen Y AD - State Key Laboratory of Chemo and Biosensing, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha, 410082, China. FAU - Wang, Kemin AU - Wang K AD - State Key Laboratory of Chemo and Biosensing, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha, 410082, China. FAU - He, Xiaoxiao AU - He X AD - State Key Laboratory of Chemo and Biosensing, College of Biology, College of Chemistry and Chemical Engineering, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Changsha, 410082, China. Electronic address: xiaoxiaohe@hnu.edu.cn. LA - eng PT - Journal Article DEP - 20261026 PL - Netherlands TA - Talanta JT - Talanta JID - 2984816R SB - IM OTO - NOTNLM OT - Colorimetric sensing OT - Diabetic wounds OT - Microneedles OT - POCT OT - Smartphone-assisted analysis COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:08 PHST- 2026/08/16 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:08 [entrez] AID - S0039-9140(26)01492-X [pii] AID - 10.1016/j.talanta.2026.130836 [doi] PST - aheadofprint SO - Talanta. 2026 Oct 26;314:130836. doi: 10.1016/j.talanta.2026.130836. PMID- 42956606 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-7072 (Electronic) IS - 0308-8146 (Linking) VI - 532 IP - Pt 4 DP - 2026 Oct 24 TI - Linking phenolic composition, cultivar and ripening stage to oxidative stability and shelf-life prediction of EVOO using accelerated aging and NIR-based chemometrics modeling. PG - 151455 LID - S0308-8146(26)03615-0 [pii] LID - 10.1016/j.foodchem.2026.151455 [doi] AB - The reliable prediction of extra virgin olive oil (EVOO) shelf-life remains a challenge for producers and regulators. This study proposes an approach combining accelerated aging with near-infrared (NIR) spectroscopy and feature selection to monitor physicochemical and sensory stability. A total of 132 samples from Arbequina, Manzanilla, Hojiblanca, Picual, and Picudo cultivars, harvested at two ripening stages and classified by phenolic content, were stored under accelerated (40  degrees C, 12 h/day light) and ambient conditions for up to 12 months. Physicochemical, sensory, and spectral data were analyzed using stepwise variable selection (SELECT) and ordinary least squares (OLS) regression. The models showed high predictive performance for free acidity (R(2) = 0.97), peroxide value (R(2) = 0.95), rancidity (R(2) = 0.94), and fruitiness (R(2) = 0.96). Phenolic-rich oils, particularly early-harvest Picual and Picudo, exhibited greater oxidative stability. The results demonstrate that accelerated aging combined with NIR-based modeling enables rapid, non-destructive, cultivar-specific shelf-life prediction. CI - Copyright (c) 2026 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Mehany, Taha AU - Mehany T AD - Department of Chemistry, University of La Rioja, Logrono, 26006, Spain. Electronic address: taha.abdellatif@unirioja.es. FAU - Gonzalez-Saiz, Jose M AU - Gonzalez-Saiz JM AD - Department of Chemistry, University of La Rioja, Logrono, 26006, Spain. Electronic address: josemaria.gonzalez@unirioja.es. FAU - Pizarro, Consuelo AU - Pizarro C AD - Department of Chemistry, University of La Rioja, Logrono, 26006, Spain. Electronic address: consuelo.pizarro@unirioja.es. LA - eng PT - Journal Article DEP - 20261024 PL - England TA - Food Chem JT - Food chemistry JID - 7702639 SB - IM OTO - NOTNLM OT - Feature selection models OT - Near infra-red spectroscopy OT - Olive oil OT - Polyphenols OT - Quality control OT - SELECT algorithm OT - Storage COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:08 PHST- 2026/02/17 00:00 [received] PHST- 2026/09/12 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:08 [entrez] AID - S0308-8146(26)03615-0 [pii] AID - 10.1016/j.foodchem.2026.151455 [doi] PST - aheadofprint SO - Food Chem. 2026 Oct 24;532(Pt 4):151455. doi: 10.1016/j.foodchem.2026.151455. PMID- 42956601 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-7072 (Electronic) IS - 0308-8146 (Linking) VI - 532 IP - Pt 4 DP - 2026 Oct 26 TI - An interpretable machine learning approach for process optimization and characterization of key model-derived parameter effects in sunflower oil diacylglycerol synthesis. PG - 151771 LID - S0308-8146(26)03931-2 [pii] LID - 10.1016/j.foodchem.2026.151771 [doi] AB - Diacylglycerol (DAG) has potential metabolic and cardiovascular health benefits. An interpretable machine learning framework was developed to predict and optimize enzymatic DAG synthesis from sunflower oil. Among 16 model configurations, XGBoost-GS was numerically top-ranked under repeated exact-condition-grouped cross-validation (overall R(2) = 0.910, RMSE = 2.942 pp., and MAE = 2.175 pp). Through process parameter optimization using differential evolution, the optimal conditions were determined to be 11.53 h, 72.11  degrees C, 8.29 wt% lipase loading, and a weight ratio of 8.93, with a predicted relative DAG content of 54.22% (ensemble predictive dispersion: 1.06%). Experimental verification at the optimization-selected condition yielded 54.68 +/- 0.20% relative DAG content. Interpretation of the final fitted model indicated that time had the highest permutation importance, temperature had the largest averaged perturbation sensitivity, and the time-weight ratio pair had the largest positive pairwise interaction metric. This framework supported model-assisted process optimization within the investigated domain. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Liu, Haoran AU - Liu H AD - School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China. FAU - Dai, Yu AU - Dai Y AD - Wuhan Institute for Food and Cosmetic control, Wuhan 430023, PR China. FAU - Yu, Huanjun AU - Yu H AD - School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China. FAU - Zhang, Siyao AU - Zhang S AD - School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China. FAU - Liao, Qiqing AU - Liao Q AD - School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China. FAU - Chen, Dong AU - Chen D AD - School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China. Electronic address: appchemcd@163.com. FAU - Jiang, Xiaoming AU - Jiang X AD - Wuhan Institute of Metrology, Standards and Quality, Wuhan 430023, PR China. FAU - Liu, Hong AU - Liu H AD - Baotou Jinlu Oil Co., Ltd., Baotou, Inner Mongolia 014000, PR China. FAU - He, Dongping AU - He D AD - School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, PR China. LA - eng PT - Journal Article DEP - 20261026 PL - England TA - Food Chem JT - Food chemistry JID - 7702639 SB - IM OTO - NOTNLM OT - Diacylglycerol OT - Lipase OT - Machine learning OT - Sunflower oil COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:08 PHST- 2026/03/24 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:08 [entrez] AID - S0308-8146(26)03931-2 [pii] AID - 10.1016/j.foodchem.2026.151771 [doi] PST - aheadofprint SO - Food Chem. 2026 Oct 26;532(Pt 4):151771. doi: 10.1016/j.foodchem.2026.151771. PMID- 42956597 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-7072 (Electronic) IS - 0308-8146 (Linking) VI - 532 IP - Pt 4 DP - 2026 Oct 26 TI - Permselective-antipathogenic-buffering ternary synergy: A grapefruit-inspired biomimetic pericarp for advanced strawberry preservation. PG - 151721 LID - S0308-8146(26)03881-1 [pii] LID - 10.1016/j.foodchem.2026.151721 [doi] AB - Inspired by the integrated defense system of grapefruit pericarp, we developed a ternary biomimetic packaging that couples permselective gas regulation, confined antimicrobial activity, and moisture buffering. The trilayer architecture consists of a dense polyamide barrier with selective O(2)/CO(2) permeability (2.7/3.6 Barrer), an in-situ-generated Ag nanoparticle layer for broad-spectrum antimicrobial protection, and a sulfonated polyethersulfone matrix enabling water-vapor modulation (143 g/(m(2).h)) and controlled Ag(+) diffusion (<100 mug/L). When applied to strawberries, this system established a protective low-O(2)/high-CO(2) atmosphere (8.4% O(2), 17.1% CO(2)), reduced respiration by 65%, and retained ~80% firmness and ~ 85% springiness after 15 days at 4  degrees C. It inhibited the tested bacterial strains by >86%, preserved chelator-soluble pectin at 1.2-fold control levels, suppressed pectinase activities and minimized malondialdehyde accumulation. This design effectively mimics natural pericarp mechanisms, offering a mechanism-driven strategy for extending the shelf life of perishable fruits. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Liao, Wenzhen AU - Liao W AD - School of Public Health / Guangdong Provincial Key Laboratory of Tropical Disease Research / BSL-3 Laboratory (Guangdong), Southern Medical University, Guangzhou, Guangdong Province, 510640, China. FAU - Xia, Xingdan AU - Xia X AD - School of Public Health / Guangdong Provincial Key Laboratory of Tropical Disease Research / BSL-3 Laboratory (Guangdong), Southern Medical University, Guangzhou, Guangdong Province, 510640, China. FAU - Wang, Wenxia AU - Wang W AD - School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, Guangdong Province, 510006, China. FAU - Wu, Weiliang AU - Wu W AD - School of Public Health / Guangdong Provincial Key Laboratory of Tropical Disease Research / BSL-3 Laboratory (Guangdong), Southern Medical University, Guangzhou, Guangdong Province, 510640, China. FAU - Dong, Hao AU - Dong H AD - College of Light Industry and Food Sciences, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong Province, 510225, China. FAU - Yang, Xingfen AU - Yang X AD - School of Public Health / Guangdong Provincial Key Laboratory of Tropical Disease Research / BSL-3 Laboratory (Guangdong), Southern Medical University, Guangzhou, Guangdong Province, 510640, China. Electronic address: Yangalice79@smu.edu.cn. FAU - He, Qi AU - He Q AD - School of Public Health / Guangdong Provincial Key Laboratory of Tropical Disease Research / BSL-3 Laboratory (Guangdong), Southern Medical University, Guangzhou, Guangdong Province, 510640, China; Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China. Electronic address: heqi18@smu.edu.cn. LA - eng PT - Journal Article DEP - 20261026 PL - England TA - Food Chem JT - Food chemistry JID - 7702639 SB - IM OTO - NOTNLM OT - Biomimetic pericarps OT - Controlled atmosphere OT - Grapefruit-inspired OT - Pectin metabolism OT - Triple multifunctional integration COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:08 PHST- 2026/02/11 00:00 [received] PHST- 2026/06/06 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:08 [entrez] AID - S0308-8146(26)03881-1 [pii] AID - 10.1016/j.foodchem.2026.151721 [doi] PST - aheadofprint SO - Food Chem. 2026 Oct 26;532(Pt 4):151721. doi: 10.1016/j.foodchem.2026.151721. PMID- 42956592 OWN - NLM STAT- Publisher LR - 20261029 IS - 1872-9800 (Electronic) IS - 0969-8043 (Linking) VI - 239 DP - 2026 Oct 27 TI - Portable X-ray imaging of simulated radiological dispersal devices. PG - 113056 LID - S0969-8043(26)00640-8 [pii] LID - 10.1016/j.apradiso.2026.113056 [doi] AB - Radiological dispersal devices (RDDs), often described as dirty bombs, represent a specific operational problem for explosive ordnance disposal (EOD) units because they combine the tactical requirements of an improvised explosive device response with radiation protection constraints. This article evaluates portable radiation detection and X-ray imaging systems under field conditions simulating radiological dispersal devices. Sealed sources of (137)Cs and (60)Co with activities ranging from 6.3 MBq to 4.74 GBq, together with a liquid (241)Am source and an Am-Be neutron source, were placed in simulated IED configurations enclosed in metal, wood, cardboard and plastic. Dose-rate measurements were performed at 20 cm and 100 cm using an identiFINDER(R) R400 and a MultiRAE Pro multi-threat monitor. Radiographic diagnostics were performed using a NOVO digital X-ray system and a DURR computed radiography system. The results show that increasing source activity and gamma-ray energy substantially affect radiation detector performance and radiographic image quality. In contrast, the low-energy alpha emitter (241)Am and the Am-Be neutron source had negligible effects on radiographic image quality despite presenting distinct detection challenges for field responders. The NOVO system maintained diagnostically useful images even in the presence of multi-giga-becquerel gamma sources, whereas the DURR system showed progressive radiation-induced image degradation, particularly for high-activity (60)Co. The findings indicate that radioactive material does not automatically prevent successful radiographic diagnostics of a suspected RDD, but the choice of imaging technology and detector has direct operational consequences for EOD response. The findings provide practical guidance for equipment selection and integrated EOD-CBRN response to suspected RDD incidents. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Rajchl, Erik AU - Rajchl E AD - NBC Defence Institute, University of Defence, Vyskov, Czech Republic. Electronic address: erik.rajchl@unob.cz. FAU - Sas, Daniel AU - Sas D AD - NBC Defence Institute, University of Defence, Vyskov, Czech Republic. FAU - Pokorny, Zbynek AU - Pokorny Z AD - Technical and Information Support Centre, 15th Engineer Regiment, Czech Armed Forces, Bechyne, Czech Republic. FAU - Bednar, Kamil AU - Bednar K AD - NBC Defence Institute, University of Defence, Vyskov, Czech Republic. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Appl Radiat Isot JT - Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine JID - 9306253 SB - IM OTO - NOTNLM OT - Dirty bomb OT - EOD OT - Portable X-ray imaging OT - Radiation detection OT - Radiation emergency OT - Radiological dispersal device COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:08 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:08 [entrez] AID - S0969-8043(26)00640-8 [pii] AID - 10.1016/j.apradiso.2026.113056 [doi] PST - aheadofprint SO - Appl Radiat Isot. 2026 Oct 27;239:113056. doi: 10.1016/j.apradiso.2026.113056. PMID- 42956591 OWN - NLM STAT- Publisher LR - 20261029 IS - 1872-9800 (Electronic) IS - 0969-8043 (Linking) VI - 239 DP - 2026 Oct 26 TI - Genotype-specific radiosensitivity and optimization of gamma irradiation dose in diverse rice germplasm using LD(50), GR(50) and multivariate analyses. PG - 113050 LID - S0969-8043(26)00634-2 [pii] LID - 10.1016/j.apradiso.2026.113050 [doi] AB - Mutation breeding is an effective approach for generating genetic variability in rice, yet its success relies on determining genotype-specific radiosensitivity to avoid the dual risks of excessive lethality or insufficient mutation induction. This study evaluated the radiosensitivity and early-stage morphological responses of 21 rice germplasms representing diverse grain types, including aromatic, biofortified red-pericarp, and high-yielding varieties, under seven irradiation doses (0-600 Gy) using a (60)Cobalt gamma source. Irradiated seeds were pre-soaked and germinated under controlled polyhouse conditions in a factorial RCBD, and germination, survivability, shoot and root length, leaf number, and fresh and dry biomass were recorded. Two-way ANOVA revealed highly significant effects (p < 0.001) for dose, germplasm, and their interaction across most of the recorded traits. Radiosensitivity varied widely among germplasms, with LD(50) values ranging from 234.7 Gy in Rod Minikit to 790.8 Gy in Rataboro and shoot length GR(50) ranging from 349.7 Gy to 581.9 Gy. Tulshimala and Rataboro exhibited the highest tolerance, whereas Rod Minikit and BRAC dhan2 were the most sensitive. Dose-response curves were fitted using the four-parameter log-logistic (LL.4) model to estimate LD(50) and GR(50), highlighting genotype-specific sensitivity to irradiation. Principal component analysis illustrated clear dose-dependent separation and genotype-specific responses, with the first two components explaining 93.6% of total variance. These results highlight the importance of genotype specific radiosensitivity assessment in rice, enabling the selection of optimal irradiation doses that maximize genetic variation and seedling survival, thereby improving the efficiency of mutation breeding and providing a practical reference for future rice improvement programs. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Perves, Masud AU - Perves M AD - Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh, 2202, Bangladesh. Electronic address: masudperves063@gmail.com. FAU - Islam, Md Tawhidul AU - Islam MT AD - Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh, 2202, Bangladesh; Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh. FAU - Islam, Md Junayed AU - Islam MJ AD - Institute of Biotechnology, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh. FAU - Sikder, Md Rayhan AU - Sikder MR AD - Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh, 2202, Bangladesh. FAU - Hassan, Mehedi AU - Hassan M AD - Electronics and Health Physics Section, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh, 2202, Bangladesh. FAU - Hasibuzzaman, Abu Sayeed Md AU - Hasibuzzaman ASM AD - Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh, 2202, Bangladesh. Electronic address: romi.hasib@gmail.com. FAU - Yasmine, Fahmina AU - Yasmine F AD - Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture, BAU Campus, Mymensingh, 2202, Bangladesh. LA - eng PT - Journal Article DEP - 20261026 PL - England TA - Appl Radiat Isot JT - Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine JID - 9306253 SB - IM OTO - NOTNLM OT - Four-parameter log-logistic (LL.4) model OT - GR(50) OT - Gamma irradiation OT - Genetic variability OT - LD(50) OT - Landrace OT - Mutation breeding OT - Oryza sativa L. OT - Radiosensitivity COIS- Declaration of competing interest The authors declare no conflicts of interest. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:08 PHST- 2026/03/24 00:00 [received] PHST- 2026/08/16 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:08 [entrez] AID - S0969-8043(26)00634-2 [pii] AID - 10.1016/j.apradiso.2026.113050 [doi] PST - aheadofprint SO - Appl Radiat Isot. 2026 Oct 26;239:113050. doi: 10.1016/j.apradiso.2026.113050. PMID- 42956583 OWN - NLM STAT- Publisher LR - 20261029 IS - 1090-2120 (Electronic) IS - 0045-2068 (Linking) VI - 184 DP - 2026 Oct 24 TI - Antibacterial activity and mechanistic insights into para-substituted triphenylphosphonium salts. PG - 110677 LID - S0045-2068(26)01213-7 [pii] LID - 10.1016/j.bioorg.2026.110677 [doi] AB - Dodecyl- and tetradecyl-substituted triphenylphosphonium (TPP) derivatives (1d and 1e) were previously identified by our group as potent antimicrobials, although their mammalian cytotoxicity and hemolytic activity limited their therapeutic potential. To mitigate these drawbacks, six novel para-substituted TPP derivatives bearing dodecyl or tetradecyl chains were synthesized by introducing electron-donating (8d, 8e) or electron-withdrawing (9d, 9e, 10d, 10e) substituents on the phenyl rings. The 4-CF(3) derivatives (10d and 10e) retained potent activity against the tested Staphylococcus aureus strains (MICs of 1-2 mug/mL) while exhibiting substantially reduced mammalian cytotoxicity compared with the parent compounds. Their biological profiles also revealed differences between mammalian cytotoxicity, hemolytic activity, and antibacterial activity across bacterial species. Mechanistic studies revealed preferential accumulation in bacterial membrane fractions, alongside effects on membrane potential and bacterial respiratory and metabolic activity. Notably, the potent anti-S. aureus activity of 10d and 10e, despite their weaker effects on membrane depolarization and bioenergetics, points to a more complex relationship between these effects and antibacterial potency. Overall, these findings highlight electronic modification of the TPP moiety as a promising strategy for modulating the biological properties of TPP-based antimicrobials and reducing mammalian cytotoxicity while retaining activity against selected bacterial pathogens. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Nunes, Barbara AU - Nunes B AD - LEPABE, ALiCE, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal. FAU - Cagide, Fernando AU - Cagide F AD - MedInUP/RISE-Health Department of Biomedicine - Unit of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal. Electronic address: fernando.fagin@med.up.pt. FAU - Vetter, Anna C AU - Vetter AC AD - Department of Chemical Biology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany. FAU - Fuchs, Hazel L S AU - Fuchs HLS AD - Department of Chemical Biology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany. FAU - Chen, Fangfang AU - Chen F AD - Department of Bioinformatics and Biochemistry, Technische Universitat Braunschweig, Rebenring 56, 38106 Braunschweig, Germany; Cellular Metabolism in Infection Research Group, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany. FAU - Fernandes, Carlos AU - Fernandes C AD - MedInUP/RISE-Health Department of Biomedicine - Unit of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal. FAU - Cordes, Thekla AU - Cordes T AD - Department of Bioinformatics and Biochemistry, Technische Universitat Braunschweig, Rebenring 56, 38106 Braunschweig, Germany; Cellular Metabolism in Infection Research Group, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany. FAU - Borges, Fernanda AU - Borges F AD - MedInUP/RISE-Health Department of Biomedicine - Unit of Pharmacology and Therapeutics, Faculty of Medicine, University of Porto, 4200-319 Porto, Portugal; Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Rua do Campo Alegre, s/n, 4169-007 Porto, Portugal. FAU - Bronstrup, Mark AU - Bronstrup M AD - Department of Chemical Biology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany; German Center for Infection Research (DZIF), Site Hannover-Braunschweig, 38124 Braunschweig, Germany; Institute of Organic Chemistry, Leibniz University Hannover, 30167 Hannover, Germany. FAU - Simoes, Manuel AU - Simoes M AD - LEPABE, ALiCE, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, s/n, 4200-465 Porto, Portugal. Electronic address: mvs@fe.up.pt. LA - eng PT - Journal Article DEP - 20261024 PL - United States TA - Bioorg Chem JT - Bioorganic chemistry JID - 1303703 SB - IM OTO - NOTNLM OT - Antibacterial agents OT - Bioenergetics OT - Electronic modulation OT - Membrane association OT - Triphenylphosphonium derivatives COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:08 PHST- 2026/07/24 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:08 [entrez] AID - S0045-2068(26)01213-7 [pii] AID - 10.1016/j.bioorg.2026.110677 [doi] PST - aheadofprint SO - Bioorg Chem. 2026 Oct 24;184:110677. doi: 10.1016/j.bioorg.2026.110677. PMID- 42956573 OWN - NLM STAT- Publisher LR - 20261029 IS - 1876-7753 (Electronic) IS - 1873-5061 (Linking) VI - 97 DP - 2026 Oct 27 TI - Establishment of four induced pluripotent stem cell lines with high polygenic risk score for Parkinson's disease. PG - 104121 LID - S1873-5061(26)00217-5 [pii] LID - 10.1016/j.scr.2026.104121 [doi] AB - Sporadic Parkinson's disease (PD) has an estimated heritability of  approximately  25%. Dermal fibroblasts from four early-onset sporadic PD patients were selected based on high PD polygenic risk scores (PRS) and reprogrammed into induced pluripotent stem cells (iPSCs) using Sendai virus. Lysosomal and microglial open chromatin PRSs were calculated. The iPSC lines exhibited normal karyotypes and STR profiles identical to the donor blood samples, expressed pluripotency-associated markers, and differentiated into derivatives of all three germ layers in vitro. These iPSC lines represent a resource for modelling polygenic, high-risk sporadic PD with a specific focus on lysosomal and microglial genetic contributions. CI - Copyright (c) 2026 The Author(s). Published by Elsevier B.V. All rights reserved. FAU - Brataas, Maja Bennin AU - Brataas MB AD - Department of Neurology, Oslo University Hospital, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway. FAU - Fjerdingstad, Hege Brincker AU - Fjerdingstad HB AD - Norwegian Center for Stem Cell Research, Department of Immunology and Transfusion Medicine, Oslo University Hospital, Oslo, Norway. FAU - Lading, Helena Homme AU - Lading HH AD - Norwegian Center for Stem Cell Research, Department of Immunology and Transfusion Medicine, Oslo University Hospital, Oslo, Norway. FAU - Micci, Francesca AU - Micci F AD - Section for Cancer Cytogenetics, Department of Pathology, The Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway. FAU - Pihlstrom, Lasse AU - Pihlstrom L AD - Department of Neurology, Oslo University Hospital, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway. FAU - Sullivan, Gareth John AU - Sullivan GJ AD - Department of Pediatric Research, Oslo University Hospital, Oslo, Norway; School of Medicine, University of St Andrews, St Andrews, UK. FAU - Glover, Joel C AU - Glover JC AD - Norwegian Center for Stem Cell Research, Department of Immunology and Transfusion Medicine, Oslo University Hospital, Oslo, Norway; Laboratory of Neural Development and Optical Recording, Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo, Norway. FAU - Toft, Mathias AU - Toft M AD - Department of Neurology, Oslo University Hospital, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway. FAU - Nordengen, Kaja AU - Nordengen K AD - Department of Neurology, Oslo University Hospital, Oslo, Norway; Department of Pharmacy, The Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway. Electronic address: kaja.nordengen@gmail.com. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Stem Cell Res JT - Stem cell research JID - 101316957 SB - IM COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:07 PHST- 2026/08/06 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:07 [entrez] AID - S1873-5061(26)00217-5 [pii] AID - 10.1016/j.scr.2026.104121 [doi] PST - aheadofprint SO - Stem Cell Res. 2026 Oct 27;97:104121. doi: 10.1016/j.scr.2026.104121. PMID- 42956572 OWN - NLM STAT- Publisher LR - 20261029 IS - 2772-9508 (Electronic) IS - 2772-9508 (Linking) VI - 192 DP - 2026 Oct 26 TI - Engineering a hypoxia-generating hydrogel to enhance the anticancer activity of prodrug Evofosfamide in lung cancer models. PG - 215270 LID - S2772-9508(26)00570-4 [pii] LID - 10.1016/j.bioadv.2026.215270 [doi] AB - Tumor hypoxia is a hallmark of solid tumors that contributes to therapeutic resistance while also creating an opportunity for selective drug activation. Hypoxia-activated prodrugs such as evofosfamide are designed to exploit these low-oxygen regions, but their clinical efficacy has been limited by the uneven and insufficient distribution of hypoxia within tumors. Here, we developed a shear-thinning hydrogel that locally reduces oxygen levels while serving as a sustained drug delivery system. The material incorporates a laccase-gallic acid enzymatic reaction that rapidly consumes oxygen and establishes hypoxic conditions within the gel, even under normoxic environments. Evofosfamide was successfully loaded into the hydrogel and exhibited controlled, near zero-order release over seven days. Over the 7-day incubation period, the hydrogel maintained its structural integrity, with Evo-loaded STHH retaining positive relative swelling, more than 80% of its initial dry mass, and an interconnected porous architecture, as confirmed by scanning electron microscopy (SEM). In an A549 lung cancer model, evofosfamide showed minimal cytotoxicity under normoxia but significantly increased activity under hypoxia. Notably, the hydrogel reproduced this effect under normoxic culture conditions by creating a localized low-oxygen environment, resulting in sustained reductions in cell viability and proliferation in both 2D and cell aggregate models. Gene expression analysis further revealed that HIF1A mRNA expression was significantly increased in the hydrogel condition compared to normoxic and hypoxic groups. Together, these results demonstrate that this platform generates localized low-oxygen conditions that enhance the activity of hypoxia-responsive therapeutics while maintaining controlled drug delivery, offering a strategy to improve the performance of hypoxia-targeted cancer treatments. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Islek, Zeynep AU - Islek Z AD - Terasaki Institute for Biomedical Innovation, 21100 Erwin St., Woodland Hills, California, 91367, United States of America; Department of Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, Yeditepe University, Istanbul, Turkiye; Department of Pharmaceutical Technology, Faculty of Pharmacy, Istanbul University-Cerrahpasa, Istanbul, Turkiye. Electronic address: zeynep.koklu@iuc.edu.tr. FAU - Jeon, Keuna AU - Jeon K AD - Terasaki Institute for Biomedical Innovation, 21100 Erwin St., Woodland Hills, California, 91367, United States of America. FAU - Ermis, Menekse AU - Ermis M AD - Terasaki Institute for Biomedical Innovation, 21100 Erwin St., Woodland Hills, California, 91367, United States of America. FAU - Taskan, Ezgi AU - Taskan E AD - Department of Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, Yeditepe University, Istanbul, Turkiye. FAU - Gudumgen, Serra Naz AU - Gudumgen SN AD - Department of Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, Yeditepe University, Istanbul, Turkiye. FAU - Sut, Pinar Akkus AU - Sut PA AD - Department of Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, Yeditepe University, Istanbul, Turkiye. FAU - Cevik, Nedim Can AU - Cevik NC AD - Department of Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, Yeditepe University, Istanbul, Turkiye. FAU - Falcone, Natashya AU - Falcone N AD - Terasaki Institute for Biomedical Innovation, 21100 Erwin St., Woodland Hills, California, 91367, United States of America. Electronic address: nfalcone@terasaki.org. LA - eng PT - Journal Article DEP - 20261026 PL - Netherlands TA - Biomater Adv JT - Biomaterials advances JID - 9918383886206676 SB - IM OTO - NOTNLM OT - Drug delivery OT - Evofosfamide OT - Hypoxic hydrogel OT - Prodrug OT - cancer therapy COIS- Declaration of competing interest The authors declare no conflict of interest. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:07 PHST- 2026/06/08 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:07 [entrez] AID - S2772-9508(26)00570-4 [pii] AID - 10.1016/j.bioadv.2026.215270 [doi] PST - aheadofprint SO - Biomater Adv. 2026 Oct 26;192:215270. doi: 10.1016/j.bioadv.2026.215270. PMID- 42956564 OWN - NLM STAT- Publisher LR - 20261029 IS - 1878-5506 (Electronic) IS - 1389-9457 (Linking) VI - 149 DP - 2026 Oct 26 TI - Reduction in reported nightmares during adjunctive trihexyphenidyl treatment in an adolescent: A case report. PG - 109338 LID - S1389-9457(26)00577-0 [pii] LID - 10.1016/j.sleep.2026.109338 [doi] AB - Nightmares are a frequent and disabling symptom of posttraumatic stress disorder (PTSD) and may perpetuate fear of sleep, insomnia, and psychiatric deterioration. Evidence for pharmacologic treatment in children and adolescents remains limited. We report a 13-year-old boy with PTSD who reported cessation of persistent nightmares during adjunctive trihexyphenidyl (THP) treatment. Polysomnography showed an apnea-hypopnea index of 9.4/h, with 24.5/h during rapid eye movement (REM) sleep. The patient and his family preferred to prioritize nightmare management and psychiatric care over treatment of obstructive sleep apnea (OSA). THP was started at 1 mg nightly and increased to 2 mg after one week. At one-month follow-up, he reported no nightmares from the first treatment night, and sleep avoidance had resolved despite receiving no OSA treatment. Nightmares recurred within days of abrupt THP discontinuation. Although this course suggests a contribution of THP, we cannot determine whether nightmares ceased or continued without being recalled after awakening. Further studies are needed to clarify its efficacy, safety, dosing, and patient selection. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Sumitomo, Noriko AU - Sumitomo N AD - Department of Child Neurology, National Center Hospital, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo, 187-8551, Japan; Department of Sleep Medicine, National Center Hospital, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo, 187-8551, Japan. FAU - Matsui, Kentaro AU - Matsui K AD - Department of Sleep Medicine, National Center Hospital, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo, 187-8551, Japan; Department of Sleep-Wake Disorders, National Institute of Mental Health, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo, 187-8551, Japan. Electronic address: matsui.kentaro@ncnp.go.jp. FAU - Tsuru, Ayumi AU - Tsuru A AD - Department of Sleep Medicine, National Center Hospital, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo, 187-8551, Japan. FAU - Matsushima, Shun AU - Matsushima S AD - Department of Sleep Medicine, National Center Hospital, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo, 187-8551, Japan. FAU - Takahashi, Eriya AU - Takahashi E AD - Department of Psychiatry, National Center Hospital, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo, 187-8551, Japan. FAU - Izuhara, Muneto AU - Izuhara M AD - Department of Sleep-Wake Disorders, National Institute of Mental Health, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo, 187-8551, Japan. FAU - Hazumi, Megumi AU - Hazumi M AD - Department of Sleep-Wake Disorders, National Institute of Mental Health, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo, 187-8551, Japan; Department of Medical Psychology, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, 113-8602, Japan. FAU - Nagao, Kentaro AU - Nagao K AD - Department of Sleep-Wake Disorders, National Institute of Mental Health, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo, 187-8551, Japan. FAU - Utsumi, Tomohiro AU - Utsumi T AD - Department of Sleep-Wake Disorders, National Institute of Mental Health, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo, 187-8551, Japan. FAU - Yoshiike, Takuya AU - Yoshiike T AD - Department of Sleep-Wake Disorders, National Institute of Mental Health, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo, 187-8551, Japan. FAU - Komaki, Hirofumi AU - Komaki H AD - Department of Child Neurology, National Center Hospital, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo, 187-8551, Japan; Translational Medical Center, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo, 187-8551, Japan. FAU - Kuriyama, Kenichi AU - Kuriyama K AD - Department of Sleep Medicine, National Center Hospital, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo, 187-8551, Japan; Department of Sleep-Wake Disorders, National Institute of Mental Health, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo, 187-8551, Japan. LA - eng PT - Journal Article DEP - 20261026 PL - Netherlands TA - Sleep Med JT - Sleep medicine JID - 100898759 SB - IM OTO - NOTNLM OT - Case report OT - Nightmares OT - PTSD OT - Pediatrics OT - Trihexyphenidyl COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Kentaro Matsui reports a relationship with Chugai Pharmaceutical Co Ltd that includes: speaking and lecture fees. Kentaro Matsui reports a relationship with Daiichi Sankyo Inc that includes: speaking and lecture fees. Kentaro Matsui reports a relationship with Eisai Co Ltd that includes: consulting or advisory and speaking and lecture fees. Kentaro Matsui reports a relationship with Japan Blood Products Organization that includes: speaking and lecture fees. Kentaro Matsui reports a relationship with Lundbeck Japan that includes: speaking and lecture fees. Kentaro Matsui reports a relationship with Meiji Seika Pharma that includes: speaking and lecture fees. Kentaro Matsui reports a relationship with Mitsubishi Tanabe Pharma that includes: speaking and lecture fees. Kentaro Matsui reports a relationship with Mochida Pharmaceutical that includes: speaking and lecture fees. Kentaro Matsui reports a relationship with MSD KK that includes: speaking and lecture fees. Kentaro Matsui reports a relationship with Nobelpharma that includes: speaking and lecture fees. Kentaro Matsui reports a relationship with Otsuka Pharmaceutical that includes: speaking and lecture fees. Kentaro Matsui reports a relationship with Sumitomo Pharma that includes: speaking and lecture fees. Kentaro Matsui reports a relationship with Takeda Pharmaceutical that includes: speaking and lecture fees. Kentaro Matsui reports a relationship with Viatris Pharmaceuticals that includes: speaking and lecture fees. Kenichi Kuriyama reports a relationship with Eisai Co Ltd that includes: consulting or advisory and lecture fees. Kenichi Kuriyama reports a relationship with Meiji Seika Pharma Co Ltd that includes: consulting or advisory and lecture fees. Kenichi Kuriyama reports a relationship with MSD KK that includes: lecture fees. Kenichi Kuriyama reports a relationship with Takeda Pharmaceutical Company Limited that includes: consulting or advisory and lecture fees. Kenichi Kuriyama reports a relationship with Shionogi and Co Ltd that includes: consulting or advisory and lecture fees. Kenichi Kuriyama reports a relationship with Sumitomo Pharma Co Ltd that includes: consulting or advisory and lecture fees. Kenichi Kuriyama reports a relationship with Daiichi Sankyo Co Ltd that includes: consulting or advisory and lecture fees. Kenichi Kuriyama reports a relationship with Accuris Healthcare LLP that includes: lecture fees. Kenichi Kuriyama reports a relationship with Taisho Pharmaceutical Co Ltd that includes: consulting or advisory and lecture fees. Kenichi Kuriyama reports a relationship with Nxera Pharma Japan Co Ltd that includes: consulting or advisory. Kenichi Kuriyama reports a relationship with Nobelpharma Co Ltd that includes: consulting or advisory and lecture fees. Kenichi Kuriyama reports a relationship with Lundbeck Japan KK that includes: consulting or advisory. Kenichi Kuriyama reports a relationship with Kissei Pharmaceutical Co Ltd that includes: consulting or advisory. Kenichi Kuriyama reports a relationship with Alfresa Pharma Co Ltd that includes: consulting or advisory. Kenichi Kuriyama reports a relationship with Sleep Healthcare Association that includes: consulting or advisory. Tomohiro Utsumi reports a relationship with Eisai that includes lecture fees, outside the submitted work. Takuya Yoshiike received lecture fees from MSD, Eisai, Mitsubishi Tanabe Pharma, Daiichi Sankyo, and Meiji Seika Pharma, outside of the submitted work. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:06 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:06 [entrez] AID - S1389-9457(26)00577-0 [pii] AID - 10.1016/j.sleep.2026.109338 [doi] PST - aheadofprint SO - Sleep Med. 2026 Oct 26;149:109338. doi: 10.1016/j.sleep.2026.109338. PMID- 42956557 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-0402 (Electronic) IS - 1367-5931 (Linking) VI - 95 DP - 2026 Oct 29 TI - Metalloprotein-inspired design of synthetic polymers. PG - 102796 LID - S1367-5931(26)00145-6 [pii] LID - 10.1016/j.cbpa.2026.102796 [doi] AB - Metal ion recognition in proteins emerges from coordinated effects of sequence patterning, hierarchical structure, and dynamic plasticity that promote high-affinity, ion-selective binding. Synthetic polymers, by contrast, offer broad chemical tunability but lack unified frameworks linking copolymer sequence and organization to function. Here, we position lanthanide-binding polymers as a tractable, technologically relevant model for importing protein-inspired design principles into synthetic macromolecules. We summarize approaches to quantify monomer patterning beyond coarse architectural classifications, enabling direct connections between polymer sequence and function. Using metalloproteins as conceptual inspiration, we examine coordination-dependent hierarchical structures in synthetic polymers, where noncovalent interactions drive reorganization across local and global length scales. We then highlight chain dynamics as an underexplored dimension of macromolecular design, where ion-dependent changes in mobility may couple to affinity and selectivity. Together, these sequence-structure-dynamics relationships provide a framework for developing polymeric chelators with protein-like metal recognition for applications in sensing, separations, and catalysis. CI - Copyright (c) 2026 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Bogen, Matthew P AU - Bogen MP AD - Department of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, United States. FAU - Swofford, William M AU - Swofford WM AD - Department of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, United States. FAU - Elder, Bridgette M AU - Elder BM AD - Department of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, United States. FAU - Knight, Abigail S AU - Knight AS AD - Department of Chemistry, The University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, United States. Electronic address: aknight@unc.edu. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - Curr Opin Chem Biol JT - Current opinion in chemical biology JID - 9811312 SB - IM COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:06 PHST- 2026/01/31 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:06 [entrez] AID - S1367-5931(26)00145-6 [pii] AID - 10.1016/j.cbpa.2026.102796 [doi] PST - aheadofprint SO - Curr Opin Chem Biol. 2026 Oct 29;95:102796. doi: 10.1016/j.cbpa.2026.102796. PMID- 42956540 OWN - NLM STAT- Publisher LR - 20261029 IS - 2352-3964 (Electronic) IS - 2352-3964 (Linking) VI - 133 DP - 2026 Oct 29 TI - Serum neurofilament light chain and the 2024 McDonald criteria for multiple sclerosis: a retrospective cohort study. PG - 106539 LID - S2352-3964(26)00423-8 [pii] LID - 10.1016/j.ebiom.2026.106539 [doi] AB - BACKGROUND: The 2024 McDonald criteria broaden the definition of multiple sclerosis (MS), enabling diagnosis in individuals with asymptomatic MRI findings or clinical presentations not meeting the 2017 criteria. We assessed whether these criteria identify such cases using independent laboratory/imaging biomarkers and whether these biomarkers predict subsequent disease activity. METHODS: Participants with asymptomatic MRI findings or clinical presentations not meeting the 2017 criteria were included and stratified according to the 2024 criteria. Serum neurofilament light chain (sNfL) and 3T brain MRI measures-including normalised brain volumes, T2 lesion load (T2LL), cortical lesion (CL) and paramagnetic rim lesion (PRL)-were compared between McDonald2024-positive and McDonald2024-negative groups, and with age/sex matched McDonald2017-positive cases according to the 2017 criteria; predictors of disease activity were assessed using penalised-Cox regression. FINDINGS: A total of 141 participants were included: 95 not meeting the 2017 criteria (n = 64 asymptomatic MRI findings; n = 31 clinical presentations), stratified as McDonald2024-positive (n = 46) or McDonald2024-negative (n = 49), and 46 McDonald2017-positive cases. McDonald2024-positive vs. McDonald2024-negative cases showed higher age-corrected sNfL Z-scores (median 0.28[IQR 0.76] vs. -0.12[0.82], p = 0.003/pFDR = 0.006), reduced normalised thalamic volume (mean 0.009+/-[SD]0.0007 vs. 0.010 +/- 0.0007, p = 0.003/pFDR = 0.006), and higher CL (1[4] vs. 0[0], p < 0.001/pFDR < 0.001) and PRL (1[1] vs. 0[0], p < 0.001/pFDR = 0.003) counts (differences remaining significant after age/sex, disease duration and T2LL adjustments). Biomarker profiles were comparable between McDonald2024-positive and McDonald2017-positive cases. Penalised-Cox regression (n = 52) identified sNfL Z-score and PRL presence as the most consistently selected predictors of disease activity, with higher bootstrap stability for sNfL (0.84) than PRL (0.76), and good model discrimination (C-index = 0.71). INTERPRETATION: The 2024 McDonald criteria identify individuals not meeting the 2017 criteria who already exhibit MS-like neuroinflammatory/neurodegenerative pathological changes. These are reflected by laboratory and imaging biomarkers, including sNfL, which may predict subsequent disease activity and complement current diagnostic frameworks to support early clinical decision-making. FUNDING: King Baudouin Foundation; Cliniques Universitaires Saint-Luc; Fondation Charcot Stichting; Fund for Scientific Research (FNRS); ECTRIMS; Biogen; Sanofi. CI - Copyright (c) 2026 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Borrelli, Serena AU - Borrelli S AD - Neuroinflammation Imaging Lab (NIL), Institute of NeuroScience, Universite catholique de Louvain, Brussels, Belgium; Department of Neurology, Hopital Erasme, Hopital Universitaire de Bruxelles, Universite Libre de Brussels, Brussels, Belgium. Electronic address: serena.borrelli@uclouvain.be. FAU - Barbuti, Elena AU - Barbuti E AD - Neuroinflammation Imaging Lab (NIL), Institute of NeuroScience, Universite catholique de Louvain, Brussels, Belgium; Department of Human Neurosciences, Sapienza University, Rome, Italy. FAU - Bayart, Jean-Louis AU - Bayart JL AD - Department of Laboratory Medicine, Clinique St-Pierre, Ottignies, Belgium; Laboratory of Neurochemistry, Institute of Neuroscience, Universite Catholique de Louvain (UCLouvain), Brussels, Belgium. FAU - Deltombe, Matthieu AU - Deltombe M AD - Laboratory of Neurochemistry, Institute of Neuroscience, Universite Catholique de Louvain (UCLouvain), Brussels, Belgium. FAU - Vanden Bulcke, Colin AU - Vanden Bulcke C AD - Neuroinflammation Imaging Lab (NIL), Institute of NeuroScience, Universite catholique de Louvain, Brussels, Belgium; ICTEAM Institute, Universite catholique de Louvain, Louvain-la-Neuve, Belgium. FAU - Stolting, Anna AU - Stolting A AD - Neuroinflammation Imaging Lab (NIL), Institute of NeuroScience, Universite catholique de Louvain, Brussels, Belgium. FAU - Guisset, Francois AU - Guisset F AD - Neuroinflammation Imaging Lab (NIL), Institute of NeuroScience, Universite catholique de Louvain, Brussels, Belgium. FAU - Tota, Vito AU - Tota V AD - Department of Neurology, CHU Helora, Universite de Mons, Mons, Belgium. FAU - Elands, Sophie AU - Elands S AD - Department of Neurology, Hopital Erasme, Hopital Universitaire de Bruxelles, Universite Libre de Brussels, Brussels, Belgium. FAU - Ceccarelli, Antonia AU - Ceccarelli A AD - Department of Neurology, Hopital Erasme, Hopital Universitaire de Bruxelles, Universite Libre de Brussels, Brussels, Belgium; Heart Rhythm Management Centre, Universitair Ziekenhuis Brussel - Vrije Universiteit Brussel, Brussels, Belgium. FAU - Bugli, Celine AU - Bugli C AD - Plateforme technologique de Support en Methodologie et Calcul Statistique, Universite catholique de Louvain, Brussels, Belgium. FAU - El Sankari, Souraya AU - El Sankari S AD - Department of Neurology, Cliniques Universitaires Saint-Luc, Universite catholique de Louvain, Brussels, Belgium. FAU - van Pesch, Vincent AU - van Pesch V AD - Laboratory of Neurochemistry, Institute of Neuroscience, Universite Catholique de Louvain (UCLouvain), Brussels, Belgium; Department of Neurology, Cliniques Universitaires Saint-Luc, Universite catholique de Louvain, Brussels, Belgium. FAU - Maggi, Pietro AU - Maggi P AD - Neuroinflammation Imaging Lab (NIL), Institute of NeuroScience, Universite catholique de Louvain, Brussels, Belgium; Department of Neurology, Cliniques Universitaires Saint-Luc, Universite catholique de Louvain, Brussels, Belgium. Electronic address: pietro.maggi@uclouvain.be. LA - eng PT - Journal Article DEP - 20261029 PL - Netherlands TA - EBioMedicine JT - EBioMedicine JID - 101647039 SB - IM OTO - NOTNLM OT - McDonald criteria OT - Multiple sclerosis OT - Neuroaxonal damage OT - Neurofilament light chains COIS- Declaration of interests S. Borrelli received speaker/consulting honoraria and/or travel grants from Sanofi, Roche, Janssen, Merck, Novartis, Alexion and Amgen, and research grants from Roche, Sanofi, and Brugmann Foundation not related to this work. JL Bayart received speaker/consulting honoraria from Siemens Healthineers and The Binding Site company. C. Vanden Bulcke received financial support by the Federation Wallonie Bruxelles-Fonds Speciaux de Recherche (F.S.R.). V. van Pesch received consulting honoraria from Biogen, Merck, Sanofi, BMS, Novartis, Janssen, Almirall, Roche, Alexion. P. Maggi received consulting honoraria from Novartis, Sanofi, Biogen and Merck. E. Barbuti, M. Deltombe, A. Stolting, F. Guisset, V. Tota, S. Elands, A. Ceccarelli, C. Bugli and S. El Sankari report no disclosures relevant to the manuscript. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:05 PHST- 2026/03/25 00:00 [received] PHST- 2026/08/11 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:05 [entrez] AID - S2352-3964(26)00423-8 [pii] AID - 10.1016/j.ebiom.2026.106539 [doi] PST - aheadofprint SO - EBioMedicine. 2026 Oct 29;133:106539. doi: 10.1016/j.ebiom.2026.106539. PMID- 42956539 OWN - NLM STAT- Publisher LR - 20261029 IS - 2352-3964 (Electronic) IS - 2352-3964 (Linking) VI - 133 DP - 2026 Oct 29 TI - Identification of peptide epitopes in major antigens of Yersinia pestis from infected humans and immunised mice. PG - 106523 LID - S2352-3964(26)00407-X [pii] LID - 10.1016/j.ebiom.2026.106523 [doi] AB - BACKGROUND: Yersinia pestis (Y. pestis), the bacterial pathogen responsible for plague in humans, remains a public health threat, particularly in light of the recent emergence of antibiotic resistance strains. METHODS: To better understand the immune response against plague and to identify potential targets for immunointervention, we performed an epitope mapping study of the IgG response against a library of peptides representing 18 Y. pestis proteins. The study included sera from 51 individuals who had recovered from plague, 26 uninfected human controls, and 20 sera from mice protected by a live-attenuated vaccine. FINDINGS: Analysis of human sera revealed 32 reactive peptides distributed across eight protein antigens, whereas analysis of mouse sera identified 187 reactive peptides spanning all 18 proteins. Combined analysis of the human and mouse IgG responses to the 32 human-reactive peptides identified 27 B-cell epitopes located within YopD, YopB, LcrV, FyuA, Pla, Ail, Caf1, and PsaA. Of these, one epitope had previously been described as protective and another as immunodominant, whereas the remaining 25 represent immunodominant epitopes identified in this study. INTERPRETATION: Interestingly, IgG antibody responses to selected YopD and YopB peptides were significantly stronger in pneumonic plague than in bubonic plague, suggesting that the distinct clinical manifestations of the disease may be associated with distinct underlying pathophysiological and molecular mechanisms. Our comprehensive mapping of linear IgG epitopes recognised by convalescent human sera, combined with prioritisation based on their predicted neutralising potential, provides a resource for future studies evaluating peptide-based diagnostics, vaccines, and monoclonal antibody therapies against plague. FUNDING: The project was supported by a grant from Agence Nationale de la Recherche (ANR). CI - Copyright (c) 2026 The Author(s). Published by Elsevier B.V. All rights reserved. FAU - Echenique-Rivera, Hebert AU - Echenique-Rivera H AD - Laboratoire Genomique, Bioinformatique, et Chimie Moleculaire, EA7528, Conservatoire National des Arts et Metiers (Cnam), Paris, France; Universite Paris Cite, Institut Pasteur Paris, Unite de Recherche Yersinia, CNRS UMR6047, F-75015, Paris, France. FAU - Simon, Arnaud AU - Simon A AD - Laboratoire Genomique, Bioinformatique, et Chimie Moleculaire, EA7528, Conservatoire National des Arts et Metiers (Cnam), Paris, France. FAU - Spadoni, Jean-Louis AU - Spadoni JL AD - Laboratoire Genomique, Bioinformatique, et Chimie Moleculaire, EA7528, Conservatoire National des Arts et Metiers (Cnam), Paris, France. FAU - Holenya, Pavlo AU - Holenya P AD - JPT Peptide Technologies GmbH, Berlin, Germany. FAU - Schnatbaum, Karsten AU - Schnatbaum K AD - JPT Peptide Technologies GmbH, Berlin, Germany. FAU - Meyniel, Jean-Philippe AU - Meyniel JP AD - Life Sciences Department, AdvanThink (ISoft), Parc des Algorithmes, Batiment Euclide, Route de l'Orme, 91190, Saint-Aubin, France. FAU - Andriatefy, Olifara AU - Andriatefy O AD - Unite d'Immunologie des Maladies Infectieuses, Institut Pasteur de Madagascar, BP 1274, 101 Antananarivo, Madagascar. FAU - Rajerison, Minoarisoa AU - Rajerison M AD - Unite Peste, Institut Pasteur de Madagascar, BP 1274, 101 Antananarivo, Madagascar. FAU - Pizarro-Cerda, Javier AU - Pizarro-Cerda J AD - Universite Paris Cite, Institut Pasteur Paris, Unite de Recherche Yersinia, CNRS UMR6047, F-75015, Paris, France; Universite Paris Cite, Yersinia National Reference Laboratory, WHO Collaborating Research & Reference Centre for Plague, FRA-140, Paris, France. FAU - Demeure, Christian AU - Demeure C AD - Universite Paris Cite, Institut Pasteur Paris, Unite de Recherche Yersinia, CNRS UMR6047, F-75015, Paris, France. FAU - Schoenhals, Matthieu AU - Schoenhals M AD - Unite d'Immunologie des Maladies Infectieuses, Institut Pasteur de Madagascar, BP 1274, 101 Antananarivo, Madagascar; Universite Paris Cite, Institut Pasteur Paris, Departement d'Immunologie, Institut Pasteur, F-75015, Paris, France. FAU - Zagury, Jean-Francois AU - Zagury JF AD - Laboratoire Genomique, Bioinformatique, et Chimie Moleculaire, EA7528, Conservatoire National des Arts et Metiers (Cnam), Paris, France; Bioinformatics Team, Peptinov, Hopital Cochin, 27 Rue du Fbg Saint-Jacques, 75014, Paris, France. Electronic address: zagury@cnam.fr. LA - eng PT - Journal Article DEP - 20261029 PL - Netherlands TA - EBioMedicine JT - EBioMedicine JID - 101647039 SB - IM OTO - NOTNLM OT - Epitope mapping OT - Humans OT - Mice OT - Plague patient OT - Vaccine OT - Yersinia pestis COIS- Declaration of interests All the authors declare that they have no conflict of interest related to this work. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:05 PHST- 2026/03/04 00:00 [received] PHST- 2026/09/11 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:05 [entrez] AID - S2352-3964(26)00407-X [pii] AID - 10.1016/j.ebiom.2026.106523 [doi] PST - aheadofprint SO - EBioMedicine. 2026 Oct 29;133:106523. doi: 10.1016/j.ebiom.2026.106523. PMID- 42956537 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-264X (Electronic) IS - 0731-7085 (Linking) VI - 284 DP - 2026 Oct 23 TI - Quantification bias in non-targeted extractables and leachables screening: A Monte Carlo comparison of uncertainty-factor and response-factor corrections. PG - 117777 LID - S0731-7085(26)00445-0 [pii] LID - 10.1016/j.jpba.2026.117777 [doi] AB - Semi-quantitative non-targeted screening of extractables and leachables (E&L) relies on surrogate standards and is therefore subject to quantification bias driven by the high variability of relative response factors (RRFs). Thus, an uncertainty factor (UF) is used to account for RRF variability, which consequently lowers the analytical evaluation threshold (AET). However, the statistical basis of the ISO 10993-18 formula, UF(ISO)= 1/(1 - RSD), with RSD the relative standard deviation of the RRFs, has not been established. The ISO formula omits a 1/mu factor (mu, mean RRF) and is defined only for RSD < 1. A Monte Carlo simulation was developed using the US FDA Chemicals List for Analytical Performance (CLAP) RRF distributions for gas chromatography-mass spectrometry (GC/MS) and for liquid chromatography-mass spectrometry (LC/MS) in positive and negative ionization modes, combined with empirical extractable concentration distributions. Multiple UF- and RRF-based correction strategies were compared across four AET levels and three surrogate concentrations. Without correction, false-negative (FN) rates ranged from 41% to 95% across analytical methods. All three CLAP datasets had RSD > 1, outside the ISO domain. Conditional correction produced simulated FN rates below 3%. These rates should however be considered optimistic, as they assume complete identification and a reliable compound-specific RRF, conditions rarely met in routine screening. Percentile-based UFs bound FNs at the selected threshold without these assumptions, although the most protective levels lowered the AET to values that are not achievable in E&L screening. A method-specific percentile UF, selected in line with the analytical instrumentation limits, is therefore proposed. CI - Copyright (c) 2026 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Yam, Sophie AU - Yam S AD - Interuniversity Institute for Biostatistics and statistical Bioinformatics (I-BioStat), Hasselt University, Agoralaan Building D, Diepenbeek 3590, Belgium. FAU - Feliciano, Rodrigo AU - Feliciano R AD - J&J Innovative Medicine, Turnhoutseweg 30, Beerse 2340, Belgium. FAU - Jaspers, Stijn AU - Jaspers S AD - Interuniversity Institute for Biostatistics and statistical Bioinformatics (I-BioStat), Hasselt University, Agoralaan Building D, Diepenbeek 3590, Belgium. Electronic address: stijn.jaspers@uhasselt.be. LA - eng PT - Journal Article DEP - 20261023 PL - England TA - J Pharm Biomed Anal JT - Journal of pharmaceutical and biomedical analysis JID - 8309336 SB - IM OTO - NOTNLM OT - Analytical evaluation threshold OT - Extractables OT - Monte Carlo simulation OT - Quantification bias OT - Relative response factor OT - Uncertainty factor COIS- Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Rodrigo Feliciano reports article publishing charges was provided by Johnson and Johnson. Sophie Yam serves as a contractor for Johnson and Johnson, outside the submitted work. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:05 PHST- 2026/08/14 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:05 [entrez] AID - S0731-7085(26)00445-0 [pii] AID - 10.1016/j.jpba.2026.117777 [doi] PST - aheadofprint SO - J Pharm Biomed Anal. 2026 Oct 23;284:117777. doi: 10.1016/j.jpba.2026.117777. PMID- 42956534 OWN - NLM STAT- Publisher LR - 20261029 IS - 1532-8708 (Electronic) IS - 0093-7754 (Linking) VI - 53 IP - 6 DP - 2026 Oct 15 TI - Immunotherapy in multiple myeloma: Molecular mechanisms, current clinical strategies, resistance, and future perspectives. PG - 152568 LID - S0093-7754(26)00113-2 [pii] LID - 10.1016/j.seminoncol.2026.152568 [doi] AB - BACKGROUND: Immunotherapy has revolutionized the treatment of multiple myeloma (MM), providing durable clinical responses through targeted activation of the immune system. However, disease relapses remain common owing to immune escape, antigen loss, T-cell exhaustion, and the immunosuppressive bone marrow microenvironment, underscoring the need for continued therapeutic innovation. AIM: This review provides a comprehensive overview of the molecular mechanisms, current immunotherapeutic strategies, resistance pathways, and future directions shaping the management of MM. METHODS: A comprehensive narrative review was conducted using PubMed, Scopus, and Web of Science to identify relevant English-language publications, including original studies, clinical trials, systematic reviews, meta-analyses, and landmark articles. The evidence was critically synthesized with emphasis on molecular mechanisms, clinical efficacy, biomarkers, therapeutic resistance, and emerging treatment strategies. RESULTS: Major immunotherapeutic modalities, including monoclonal antibodies, antibody-drug conjugates, bispecific antibodies, CAR-T cell therapy, vaccines, and NK-cell-based approaches, have substantially improved outcomes for patients with relapsed or refractory MM. B cell maturation antigen-directed therapies have demonstrated particularly high response rates, while emerging targets such as GPRC5D and FcRH5 are expanding therapeutic options. Nevertheless, treatment durability is limited by tumor heterogeneity, antigen modulation, immune dysfunction, and therapy-related toxicities. Emerging strategies-including multi-antigen targeting, rational combination regimens, optimized treatment sequencing, biomarker-guided patient selection, and integration of real-world and health-economic evidence-may further improve long-term outcomes. CONCLUSION: Immunotherapy has fundamentally reshaped MM management, yet durable disease control remains a major challenge. Advances in precision immunotherapy, next-generation cellular engineering, predictive biomarkers, and personalized combination strategies are expected to overcome resistance, extend remission, and establish more effective and sustainable treatment paradigms. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Mustafa, Yasser Fakri AU - Mustafa YF AD - Department of Pharmaceutical Chemistry, College of Pharmacy, University of Mosul, Mosul, 41001, Iraq. Electronic address: Dr.yassermustafa@uomosul.edu.iq. LA - eng PT - Journal Article PT - Review DEP - 20261015 PL - United States TA - Semin Oncol JT - Seminars in oncology JID - 0420432 SB - IM OTO - NOTNLM OT - Bispecific antibodies OT - CAR-T cell OT - Immunotherapy OT - Multiple myeloma OT - Tumor microenvironment COIS- Declaration of competing interest The author declares that he has no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:05 PHST- 2026/08/12 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:05 [entrez] AID - S0093-7754(26)00113-2 [pii] AID - 10.1016/j.seminoncol.2026.152568 [doi] PST - aheadofprint SO - Semin Oncol. 2026 Oct 15;53(6):152568. doi: 10.1016/j.seminoncol.2026.152568. PMID- 42956522 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-2448 (Electronic) IS - 0043-1354 (Linking) VI - 309 IP - Pt B DP - 2026 Oct 24 TI - Bridged peracetic acid adsorption at a Co-O(v)-Ce defect interface redirects activation toward singlet-oxygen-dominated water purification. PG - 127209 LID - S0043-1354(26)01881-6 [pii] LID - 10.1016/j.watres.2026.127209 [doi] AB - Peracetic acid (PAA)-based advanced oxidation processes are attractive for water purification because of their environmental compatibility and facile activation. However, poorly controlled interfacial adsorption and O-O activation can promote indiscriminate radical release, decoupling PAA consumption from selective contaminant oxidation. Here, we constructed a Co-O(v)-Ce defect interface that induced bridged PAA adsorption and redirected activation toward a surface-mediated, (1)O(2)-dominated pathway. The resulting system exhibited a k(obs) of 0.322 min(-1), 5.6 times that of Co-CeO(2)/PAA, and increased PAA productivity to 208.1 mg Naproxen(NPX) mmol(-1) PAA, representing a 35.8% improvement. The defect interface enabled Co and vacancy-associated Ce to cooperatively anchor the two peroxide oxygens, promoting charge redistribution across the O-O unit and stabilizing oxygenated intermediates at the surface. Consequently, the reaction shifted from one-end radical release toward a lower-energy interfacial oxygen-rearrangement sequence through *O/*OOH/*O(2), thereby suppressing radical escape and favoring (1)O(2) formation. The catalyst maintained 94.9-99.6% NPX removal in tap, lake, and seawater and sustained 95-98% removal during 1200 min continuous operation. These findings establish defect-coupled adsorption geometry as a strategy for controlling oxidant fate and enabling selective, resource-efficient PAA-based water purification. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Ding, Wenqing AU - Ding W AD - School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, PR China. FAU - Gao, Chao AU - Gao C AD - MOE Key Laboratory of Pollution Processes and Environmental Criteria, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin, 300350, PR China. FAU - Li, Xiguang AU - Li X AD - School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, PR China. FAU - Sun, Peizhe AU - Sun P AD - School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, PR China. FAU - Wu, Shikang AU - Wu S AD - School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, PR China. FAU - Jia, Xiangkun AU - Jia X AD - School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, PR China. FAU - Kong, Kaizhou AU - Kong K AD - School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, PR China. FAU - Yu, Guanqi AU - Yu G AD - School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, PR China. FAU - Wang, Pengfei AU - Wang P AD - School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, PR China; MOE Key Laboratory of Pollution Processes and Environmental Criteria, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin, 300350, PR China. Electronic address: pengfeiwang@nankai.edu.cn. FAU - Zhan, Sihui AU - Zhan S AD - School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, PR China. Electronic address: shzhan@tju.edu.cn. LA - eng PT - Journal Article DEP - 20261024 PL - England TA - Water Res JT - Water research JID - 0105072 SB - IM OTO - NOTNLM OT - Bridged adsorption OT - Co-O(v)-Ce defect interface OT - Oxidant productivity OT - Oxygen vacancy OT - Singlet oxygen OT - Water purification OT - peracetic acid COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:05 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:05 [entrez] AID - S0043-1354(26)01881-6 [pii] AID - 10.1016/j.watres.2026.127209 [doi] PST - aheadofprint SO - Water Res. 2026 Oct 24;309(Pt B):127209. doi: 10.1016/j.watres.2026.127209. PMID- 42956508 OWN - NLM STAT- Publisher LR - 20261029 IS - 1476-928X (Electronic) IS - 1476-9271 (Linking) VI - 126 IP - Pt 2 DP - 2026 Oct 24 TI - Integrated transcriptomic, machine-learning, and molecular simulation analysis prioritizes isovitexin-HSP90AB1 and cajanone-SERPINE1 interactions in hepatocellular carcinoma. PG - 109498 LID - S1476-9271(26)00625-0 [pii] LID - 10.1016/j.compbiolchem.2026.109498 [doi] AB - Hepatocellular carcinoma (HCC) remains a major cause of cancer mortality, highlighting the need for computational strategies that integrate disease transcriptomics with compound-target prediction and molecular simulation. This study investigated potential anti-HCC mechanisms of Cajanus cajan constituents through an integrated workflow. HCC-associated differentially expressed genes from TCGA-LIHC and three Gene Expression Omnibus datasets (GSE144269, GSE94660, and GSE14520) were intersected with predicted targets of selected C. cajan compounds. Protein-protein interaction analysis, functional enrichment, supervised machine-learning models, external validation, survival analysis, molecular docking, density functional theory calculations, molecular dynamics (MD) simulations, and MM/GBSA free-energy estimation were then applied. The integrative analyses prioritized a nine-gene network and identified HSP90AB1 and ESR1 as survival-associated markers, whereas SERPINE1 showed a nonsignificant risk trend. LASSO, random forest, and support vector machine classifiers displayed strong discrimination in the external dataset (area under the receiver operating characteristic curve, 0.981, 0.948, and 0.981, respectively). Docking ranked isovitexin-HSP90AB1 and cajanone-SERPINE1 among the favorable compound-target pairs. Primary 500-ns MD simulations, independent 100-ns replicates, and MM/GBSA analyses supported persistent interactions and stable conformational behavior for both complexes. These findings prioritize two testable C. cajan compound-protein hypotheses for HCC and provide a computational basis for subsequent biochemical and cellular validation. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Fan, Huiqin AU - Fan H AD - Department of Traditional Chinese Medicine, Yangpu Hospital, School of Medicine, Tongji University, No. 450 Tengyue Road, Shanghai, Yangpu District, 200090, China. FAU - Duan, Jicheng AU - Duan J AD - Department of Hepatobiliary and Pancreatic Surgery, The Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, No. 358 Datong Road, Pudong New Area, Shanghai, 200137, China. FAU - Wu, Xiaoxiong AU - Wu X AD - Department of Traditional Chinese Medicine, Yangpu Hospital, School of Medicine, Tongji University, No. 450 Tengyue Road, Shanghai, Yangpu District, 200090, China. Electronic address: beary999@163.com. LA - eng PT - Journal Article DEP - 20261024 PL - England TA - Comput Biol Chem JT - Computational biology and chemistry JID - 101157394 SB - IM OTO - NOTNLM OT - Cajanus cajan OT - Hepatocellular carcinoma OT - MM/GBSA OT - Machine learning OT - Molecular dynamics OT - Transcriptomics COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:04 PHST- 2026/08/26 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:04 [entrez] AID - S1476-9271(26)00625-0 [pii] AID - 10.1016/j.compbiolchem.2026.109498 [doi] PST - aheadofprint SO - Comput Biol Chem. 2026 Oct 24;126(Pt 2):109498. doi: 10.1016/j.compbiolchem.2026.109498. PMID- 42956507 OWN - NLM STAT- Publisher LR - 20261029 IS - 1476-928X (Electronic) IS - 1476-9271 (Linking) VI - 126 IP - Pt 2 DP - 2026 Oct 26 TI - Cost-effectiveness analysis of Mpox control policies: A hybrid rough set-machine learning approach. PG - 109502 LID - S1476-9271(26)00629-8 [pii] LID - 10.1016/j.compbiolchem.2026.109502 [doi] AB - Mpox remains a major global public health challenge, with ongoing human-to-human transmission and increased severity from emerging viral clades, despite vaccine availability. We introduce a non-autonomous deterministic mathematical model to examine effective and economical Mpox policy across all 31 possible intervention combinations. The Mpox model incorporates five time-dependent interventions: vaccination, awareness, isolation, hospitalization, and booster dose vaccination, linked to transmission reduction and policy evaluation. We estimate the model parameters from Mpox outbreak data and determine the basic reproduction number. Sensitivity analysis identifies human transmission rate, vaccination rate, and vaccine failure rate as the most influential parameters affecting transmission. We establish the existence of optimal controls and derive the corresponding optimality conditions. We numerically evaluate 31 intervention policies using the forward-backwards sweep method over 90 days. Implementing all five controls yields the highest effectiveness, with an efficiency index of 99.37%, while awareness alone achieves 94.30%. The average cost-effectiveness ratio (ACER) analysis demonstrates that awareness alone is the most cost-effective policy. However, ACER only ranks policies and does not show policymakers under which budget and impact conditions a policy remains cost-effective. To address this gap, we combine rough set theory (RST) with machine learning methods. The RST identifies intervention cost as the core attribute and generates IF-THEN decision rules linking budget level and expected infections averted to cost-effectiveness class, helping policymakers select interventions under economic constraints. The hybrid RST-neural network model achieves the highest classification accuracy (approximately 93%), confirming the consistency of the extracted rules. These results provide a transparent, data-driven framework to help public health authorities develop effective Mpox prevention and control policies. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Sinha, Arvind Kumar AU - Sinha AK AD - Department of Mathematics, National Institute of Technology Raipur, Raipur, Chhattisgarh, India. Electronic address: aksinha.maths@nitrr.ac.in. FAU - Namdev, Nishant AU - Namdev N AD - Department of Basic Science, Indian Institute of Information Technology, Nagpur, Maharashtra, India. Electronic address: nishantnamdev84@yahoo.in. FAU - Kushavaha, Sunil Kanhaiyalal AU - Kushavaha SK AD - Thakur Shree DPS College of Engineering and Management, Vasai East, Maharashtra, India. Electronic address: kushavaha.sunil@gmail.com. LA - eng PT - Journal Article DEP - 20261026 PL - England TA - Comput Biol Chem JT - Computational biology and chemistry JID - 101157394 SB - IM OTO - NOTNLM OT - Economic methodology OT - Efficiency index OT - Mpox OT - Optimal control OT - Rough set decision framework COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:04 PHST- 2026/08/11 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:04 [entrez] AID - S1476-9271(26)00629-8 [pii] AID - 10.1016/j.compbiolchem.2026.109502 [doi] PST - aheadofprint SO - Comput Biol Chem. 2026 Oct 26;126(Pt 2):109502. doi: 10.1016/j.compbiolchem.2026.109502. PMID- 42956503 OWN - NLM STAT- Publisher LR - 20261029 IS - 1476-928X (Electronic) IS - 1476-9271 (Linking) VI - 126 IP - Pt 2 DP - 2026 Oct 27 TI - Enhancing tumor T-cell antigen prediction using cross-attention-based multimodal representations with hybrid CNN-GLU model. PG - 109519 LID - S1476-9271(26)00646-8 [pii] LID - 10.1016/j.compbiolchem.2026.109519 [doi] AB - Tumor T-cell antigens play a significant role in cancer immunotherapy by enabling the immune system to recognize and eliminate malignant cells. However, existing experimental methods for identifying these antigens are costly, time-consuming, and labor-intensive, highlighting the need for an accurate computational prediction model. In this study, we propose iTTCA-DeepGLU, a deep-learning model for predicting tumor T-cell antigens from peptide sequences. Each peptide sequence was initially transformed into an SMR-based two-dimensional matrix, after which local phase quantization was applied to generate novel intrinsic local descriptors, termed SMR-LPQ features. In parallel, semantic and contextual features were extracted using the pretrained ProtT5 protein language model. The SMR-LPQ and ProtT5 features were then integrated through cross-attention-based fusion to construct a multimodal feature representation. Subsequently, a gradient-boosted decision tree was applied to retain the most informative features. Among the applied classifiers, our hybrid CNN-GLU model demonstrated high predictive performance. The proposed iTTCA-DeepGLU model using Train-DS1 and Train-DS2 datasets achieved accuracies of 95.87% and 96.72%, representing improvements of 8% and 11%, respectively, with corresponding AUC values of 0.97 and 0.98. To evaluate the model's generalizability, it was further assessed using two independent test sets. On the imbalanced Ind-DS1 dataset, the iTTCA-DeepGLU model achieved an accuracy of 90.39%, representing a 14% improvement, with an AUC of 0.94. Similarly, on the balanced Ind-DS2 dataset. iTTCA-DeepGLU attained an accuracy of 95.30%, approximately 2% higher than that of existing tumor T-cell antigen prediction models, with an AUC of 0.92. Overall, iTTCA-DeepGLU demonstrated strong predictive performance and generalization capability, highlighting its potential as a reliable computational tool for identifying tumor T-cell antigens and supporting cancer immunotherapy research. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Raza, Ali AU - Raza A AD - School of Electronic and Communication Engineering, Shenzhen Polytechnic University, Shenzhen, 518055, China; School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China. FAU - Akbar, Shahid AU - Akbar S AD - Department of Information Technology, The University of Haripur, Haripur, Pakistan. Electronic address: shahid.akbar@uoh.edu.pk. FAU - Khan, Ghufran Ahmad AU - Khan GA AD - School of Electronic and Communication Engineering, Shenzhen Polytechnic University, Shenzhen, 518055, China; School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China. FAU - Alghamdi, Wajdi AU - Alghamdi W AD - Department of Information Technology, Faculty of Computing and Information Technology, King Abdulaziz University, Jeddah, 21589, Saudi Arabia. FAU - Luo, Ximei AU - Luo X AD - Institute of Fundamental and Frontier Sciences, University of Electronic Science and Technology of China, Chengdu, China. FAU - Xu, Lei AU - Xu L AD - School of Electronic and Communication Engineering, Shenzhen Polytechnic University, Shenzhen, 518055, China. Electronic address: csleixu@szpu.edu.cn. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Comput Biol Chem JT - Computational biology and chemistry JID - 101157394 SB - IM OTO - NOTNLM OT - Convolutional neural network OT - Cross-attention fusion OT - GBDT feature selection OT - Gate linear unit OT - Hybrid deep model OT - Tumor T-cell antigens COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:04 PHST- 2026/08/08 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:04 [entrez] AID - S1476-9271(26)00646-8 [pii] AID - 10.1016/j.compbiolchem.2026.109519 [doi] PST - aheadofprint SO - Comput Biol Chem. 2026 Oct 27;126(Pt 2):109519. doi: 10.1016/j.compbiolchem.2026.109519. PMID- 42956497 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-4529 (Electronic) IS - 0952-8180 (Linking) VI - 116 DP - 2026 Oct 29 TI - Short-term peripheral nerve stimulation for pain relief in adults: A systematic review and meta-analysis of randomized controlled trials. PG - 112352 LID - S0952-8180(26)00231-X [pii] LID - 10.1016/j.jclinane.2026.112352 [doi] AB - BACKGROUND: Short-term peripheral nerve stimulation serves as a neuromodulation therapy that modulates neural activity to provide therapeutic benefit, particularly pain relief. This study investigated the effectiveness of short-term peripheral nerve stimulation as a minimally invasive approach for achieving pain relief. METHODS: We systematically searched PubMed, Embase, Web of Science, Scopus, MEDLINE, Cochrane Library, and Google Scholar from database inception till September 10, 2026, for randomized controlled trials (RCTs) in adults comparing short-term peripheral nerve stimulation with sham stimulation or usual care. The primary outcome was the change in average pain intensity during the implantation period. RESULTS: Eight RCTs (543 participants) were included. Compared with the control group, the intervention group showed significant reductions in average pain intensity during the implantation period at 1-4 weeks (SMD = -0.48, 95% CI = -0.71 to -0.26, P < 0.0001) and 5-8 weeks (SMD = -0.96, 95% CI = -1.22 to -0.70, P < 0.0001), but no significant result after electrode removal. For worst pain intensity, the intervention group showed a greater reduction than the control group at all time periods except 1-4 weeks after EOT. Improvements in pain interference were delayed, becoming significant at 5-8 weeks during the implantation period (MD = -1.34, 95% CI = -1.86 to -0.82, P < 0.00001) and >/=8 weeks after the end of treatment (MD = -1.09, 95%CI = -1.69 to -0.50, P = 0.0003). Subgroup analyses showed improvements in postoperative pain. The usual care-controlled subgroup showed more pronounced and sustained benefits than the sham-controlled subgroup, and the chronic pain subgroup also showed more pronounced and sustained benefits compared to the acute/subacute pain subgroup. CONCLUSION: This meta-analysis provided preliminary evidence that short-term peripheral nerve stimulation may relieve pain during the implantation period, but not all outcomes suggest sustained improvements after electrode removal. REGISTRATION PROSPERO: CRD420251243978. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Bai, Wentao AU - Bai W AD - Department of Anaesthesiology, School of Clinical Medicine, The University of Hong Kong, Hong Kong. FAU - Cheung, Chi Wai AU - Cheung CW AD - Department of Anaesthesiology, School of Clinical Medicine, The University of Hong Kong, Hong Kong; Gleneagles Hospital, Hong Kong. Electronic address: cheucw@hku.hk. FAU - Bao, Yuchang AU - Bao Y AD - Department of Anaesthesiology, School of Clinical Medicine, The University of Hong Kong, Hong Kong. FAU - Feng, Zhiying AU - Feng Z AD - Department of Pain Medicine, College of Medicine, First Affiliated Hospital, Zhejiang University, Zhejiang, China. FAU - Liao, Xiang AU - Liao X AD - Department of Pain Medicine, Shenzhen Nanshan People's Hospital, Shenzhen, China. FAU - Ho, Hung Chak AU - Ho HC AD - Department of Public and International Affairs, City University of Hong Kong, Hong Kong; Institute of Global Governance and Innovation for a Shared Future, City University of Hong Kong, Hong Kong. Electronic address: hungcho2@cityu.edu.hk. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - United States TA - J Clin Anesth JT - Journal of clinical anesthesia JID - 8812166 SB - IM OTO - NOTNLM OT - Neuromodulation OT - Pain OT - Pain management OT - Peripheral nerve stimulation OT - meta-analysis COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:04 PHST- 2026/04/18 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:04 [entrez] AID - S0952-8180(26)00231-X [pii] AID - 10.1016/j.jclinane.2026.112352 [doi] PST - aheadofprint SO - J Clin Anesth. 2026 Oct 29;116:112352. doi: 10.1016/j.jclinane.2026.112352. PMID- 42956495 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-2828 (Electronic) IS - 1350-4177 (Linking) VI - 134 DP - 2026 Oct 22 TI - Natural deep eutectic solvent ultrasound extraction and imprinting recognition for sustainable harvesting of flavonoid aglycones from safflower: Experimental and multi-scale simulations. PG - 108128 LID - S1350-4177(26)00393-7 [pii] LID - 10.1016/j.ultsonch.2026.108128 [doi] AB - Flavonoid aglycones derived from safflower are valuable antioxidants, however, conventional extraction and separation methods rely on large volumes of organic solvents and suffer from poor selectivity. Therefore, this study developed a new integrated strategy that combines ultrasound-assisted natural deep eutectic solvent (NADES) green extraction with molecularly imprinted polymer specific separation, achieving enrichment and specific separation of flavonoid aglycones in safflower. A choline chloride-based NADES was screened as the optimal extractant under ultrasonic assistance (40 kHz, 100 W), with the extraction yield of flavonoid aglycones from safflower reaching as high as 5.37 mg/g. Acoustic cavitation-bubble oscillation, growth, and implosive collapse-generates shear forces, micro-jets, and turbulence that overcome the high viscosity of NADES, facilitating solvent penetration and mass transfer. Density functional theory (DFT) and molecular dynamics (MD) simulations revealed hydrogen bonding and van der Waals interactions as the primary driving forces governing extraction. Guided by DFT-predicted template-to-monomer ratios, a surface molecularly imprinted polymer (SMIP) with specific selectivity for flavonoid aglycones was fabricated on MOF SU-102, exploiting its large surface area and mesoporous structure to overcome the low capacity of traditional MIPs. The resulting SMIP exhibited an adsorption capacity as high as 44.639 mg/g and a maximum imprinting factor of 1.80. Machine learning (ML) was employed to optimize adsorption conditions, reducing experimental effort and cost. The NADES extract was processed through a SMIP-packed syringe-based SPE cartridge, which effectively eliminated interference from complex matrices and efficiently enriched flavonoid aglycones, followed by HPLC detection. The method achieved excellent precision (RSD = 0.318%) and maintained > 90% recovery after seven adsorption-desorption cycles. This NADES-SMIP-SPE platform offers a green, selective, and reusable route for safflower valorization and isolation of bioactive compounds from complex plant matrices. In addition, the green NADES and recyclable, highly selective SMIP can help reduce energy consumption and costs in the industrial separation of plant active ingredients, showing promising potential for scalable and industrial application. CI - Copyright (c) 2026 The Author(s). Published by Elsevier B.V. All rights reserved. FAU - Liu, Zhiliu AU - Liu Z AD - School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; Tianjin Key Laboratory of Therapeutic Substance of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. FAU - Wang, Kechun AU - Wang K AD - School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; Tianjin Key Laboratory of Therapeutic Substance of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. FAU - Zheng, Xiaoqing AU - Zheng X AD - School of Chinese Medicine and Pharmaceutical Engineering, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. FAU - Zhao, Xi AU - Zhao X AD - School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; Tianjin Key Laboratory of Therapeutic Substance of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. FAU - Jia, Yidan AU - Jia Y AD - School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; Tianjin Key Laboratory of Therapeutic Substance of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. FAU - Wang, Yan AU - Wang Y AD - School of Chinese Medicine and Pharmaceutical Engineering, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. FAU - Xiang, Beibei AU - Xiang B AD - School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. Electronic address: xiangbeibei03230@163.com. FAU - Wu, Lijie AU - Wu L AD - School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. Electronic address: wulj0816@tjutcm.edu.cn. FAU - Li, Xiaoxia AU - Li X AD - School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; State Key Laboratory of Chinese Medicine Modernization, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; Tianjin Key Laboratory of Therapeutic Substance of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; China-Ghana Belt and Road Joint Laboratory on Traditional Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. Electronic address: lixx@tjutcm.edu.cn. LA - eng PT - Journal Article DEP - 20261022 PL - Netherlands TA - Ultrason Sonochem JT - Ultrasonics sonochemistry JID - 9433356 SB - IM OTO - NOTNLM OT - Flavonoid aglycones OT - Molecularly imprinted polymers OT - Multi-scale simulations OT - Natural deep eutectic solvents OT - Safflower OT - Sonoprocessing COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:04 PHST- 2026/07/21 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:04 [entrez] AID - S1350-4177(26)00393-7 [pii] AID - 10.1016/j.ultsonch.2026.108128 [doi] PST - aheadofprint SO - Ultrason Sonochem. 2026 Oct 22;134:108128. doi: 10.1016/j.ultsonch.2026.108128. PMID- 42956493 OWN - NLM STAT- Publisher LR - 20261029 IS - 1532-3072 (Electronic) IS - 0040-8166 (Linking) VI - 104 IP - Pt 3 DP - 2026 Oct 20 TI - Immune imbalance and inflammatory pathways in hypertensive disorders of pregnancy: Consequences for embryonic and fetal development. PG - 104071 LID - S0040-8166(26)00767-6 [pii] LID - 10.1016/j.tice.2026.104071 [doi] AB - Hypertensive disorders of pregnancy (HDP), including preeclampsia, remain a major cause of maternal and perinatal morbidity and mortality worldwide, affecting 5-10% of pregnancies. This review synthesizes evidence positioning immune dysregulation as a continuous developmental determinant linking peri-implantation maladaptation to placental dysfunction, systemic endothelial injury, and long-term fetal programming. Successful pregnancy requires tightly coordinated maternal-fetal immune tolerance, mediated by decidual natural killer cells, regulatory T cells (Tregs), M2-polarized macrophages, tolerogenic dendritic cells, and cytokine/checkpoint networks (IL-10, TGF-beta, LIF, PD-1/PD-L1). Failure of this staged adaptation reduced Treg frequency and function, Th1/Th17 dominance, cytotoxic NK-cell skewing, pro-inflammatory macrophage polarization, inflammasome (NLRP3) activation, complement dysregulation, and AT1-receptor autoantibodies impairs spiral-artery remodeling and trophoblast invasion. The resulting placental hypoxia, oxidative stress, and anti-angiogenic imbalance (elevated sFlt-1/PlGF) drive the maternal syndrome while releasing pathogenic extracellular vesicles that disseminate inflammatory signals. These processes may contribute to fetal growth restriction and long-term neurodevelopmental and cardiometabolic vulnerability. Epidemiological studies have reported associations between maternal HDP, particularly preeclampsia, and subsequent neurodevelopmental outcomes including autism-spectrum disorder; however, these associations are observational, effect sizes are modest, and residual confounding by prematurity, fetal growth restriction, maternal metabolic and psychiatric conditions, socioeconomic factors, and familial susceptibility remains possible. Accordingly, current evidence does not establish that preeclampsia directly causes autism-spectrum disorder. Therapeutic opportunities include low-dose aspirin, agents targeting angiogenic or inflammasome pathways, and experimental exosome-based approaches, with future precision strategies matching interventions to immune endotypes. Bridging traditionally separate domains of immunology, placentology, and developmental programming, this synthesis highlights the maternal-placental-fetal immune axis as a unified continuum and identifies opportunities for earlier prevention and endotype-guided therapy to reduce the intergenerational burden of HDP. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Dai, Huixi AU - Dai H AD - Department of Obstetrics and Gynecology, Affiliated Wenzhou People's Hospital, Hangzhou Medical College, Wenzhou, China. Electronic address: yeqianxue@yeah.net. FAU - Xu, Haixia AU - Xu H AD - Department of Obstetrics and Gynecology, Affiliated Wenzhou People's Hospital, Hangzhou Medical College, Wenzhou, China. Electronic address: 15258635378@163.com. LA - eng PT - Journal Article PT - Review DEP - 20261020 PL - Scotland TA - Tissue Cell JT - Tissue & cell JID - 0214745 SB - IM OTO - NOTNLM OT - Biomarkers OT - Fetal Development OT - Hypertension OT - Immunity OT - Inflammation OT - Natural Killer Cells OT - Placenta OT - Preeclampsia OT - Pregnancy OT - T Lymphocytes COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:04 PHST- 2026/08/12 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:04 [entrez] AID - S0040-8166(26)00767-6 [pii] AID - 10.1016/j.tice.2026.104071 [doi] PST - aheadofprint SO - Tissue Cell. 2026 Oct 20;104(Pt 3):104071. doi: 10.1016/j.tice.2026.104071. PMID- 42956474 OWN - NLM STAT- Publisher LR - 20261029 IS - 1944-8252 (Electronic) IS - 1944-8244 (Linking) DP - 2026 Oct 30 TI - Efficient Hydroxymethanesulfonate Electrosynthesis from CO2 and Sulfite over CuPd1 Single-Atom Alloys. LID - 10.1021/acsami.6c19144 [doi] AB - Electrochemical C-S coupling of CO2 and sulfite (SO32-) offers a sustainable alternative for hydroxymethanesulfonate (HMS) synthesis, yet its electrocatalytic selectivity remains far from satisfactory. Here, CuPd1 single-atom alloys (CuPd1-SAA) are developed as a highly efficient catalyst for HMS electrosynthesis, delivering an exceptional Faradaic efficiency of 56.8% and an HMS yield rate of 301.5 mmol h-1 g-1 in an MEA electrolyzer, together with outstanding electrolysis stability for over 200 h of electrolysis. Mechanistic investigations reveal the critical Cu-Pd1 synergy in driving CO2 reduction to *CHOH and C-S coupling of *CHOH with *SO3 for selective HMS generation. CI - (c) 2026 American Chemical Society. FAU - Wu, Tingting AU - Wu T AD - College of Science, Hebei North University, Zhangjiakou, Hebei075000, China. FAU - Wang, Jiang AU - Wang J AD - School of Information Technology and Engineering, Hebei North University, Zhangjiakou, Hebei075000, China. FAU - Zhang, Fengyu AU - Zhang F AD - College of Science, Hebei North University, Zhangjiakou, Hebei075000, China. FAU - Yang, Ruixuan AU - Yang R AD - School of Materials Science and Engineering, Lanzhou Jiaotong University, Lanzhou730070, China. FAU - Tian, Ye AU - Tian Y AD - College of Science, Hebei North University, Zhangjiakou, Hebei075000, China. FAU - Chu, Ke AU - Chu K AUID- ORCID: 0000-0002-0606-2689 AD - School of Materials Science and Engineering, Lanzhou Jiaotong University, Lanzhou730070, China. LA - eng GR - JYT2024004/Department of Education of Hebei Province/ PT - Journal Article DEP - 20261030 PL - United States TA - ACS Appl Mater Interfaces JT - ACS applied materials & interfaces JID - 101504991 SB - IM OTO - NOTNLM OT - CO2 electroreduction OT - CuPd catalyst OT - C-S coupling OT - electrosynthesis OT - hydroxymethanesulfonate OT - membrane electrode assembly OT - single-atom alloy EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:03 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/26 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:03 [entrez] AID - 5461302 [pii] AID - 10.1021/acsami.6c19144 [doi] PST - aheadofprint SO - ACS Appl Mater Interfaces. 2026 Oct 30. doi: 10.1021/acsami.6c19144. PMID- 42956467 OWN - NLM STAT- Publisher LR - 20261029 IS - 1361-648X (Electronic) IS - 0953-8984 (Linking) DP - 2026 Oct 29 TI - Cavity-Assisted Electrostatic Braiding and Readout of Fractional Quantum Hall Anyons. LID - 10.1088/1361-648X/aebb85 [doi] AB - We introduce a cavity-assisted near-field electrostatic architecture for conditional braiding of localized fractional quantum Hall quasiholes and heterodyne probing of braid-sensitive signatures, with particular relevance to candidate non-Abelian quasiholes at filling factor $\nu=5/2$. Counter-propagating cavity branches select oppositely rotating electrostatic trap trajectories, so the photonic state controls braid orientation. We derive the leading cavity cross-coherence and the conditions for bounded quasihole following, including two-dimensional confinement, mode selectivity, adiabaticity, and phase stability. For Ising anyons, the symmetric inverse-branch protocol yields only a calibrated phase for every pure braid and state, and is therefore blind to non-Abelian information. An asymmetric branch/reference protocol instead accesses a single braid power; normalized two-mode heterodyne cross-coherence reveals the braid through suppression of the braid-run first moment relative to a resolved nonzero matched trivial reference, when the Abelian phase is stabilized or tracked. A computational-fusion-basis-diagonal preparation suffices for the minimal Ising contrast; coherent fusion-space extensions require additional state control. A cylindrical-Hankel boundary-value calculation resolves the downstream trap profile and surface-to-2DEG transfer over a radius--depth window for a prescribed slow annular harmonic, while its microscopic cavity--gate generation remains device specific. Within the stated models, the framework provides testable predictions for electrostatic transfer, cavity back-action, and matched-control cross-coherence, while complete device feasibility requires independent establishment of the remaining implementation requirements. CI - (c) 2026 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved. FAU - Moriya, Netzer AU - Moriya N AUID- ORCID: 0009-0004-3693-1971 AD - Development, siOnet Ltd., 6 Yair St., Tel0523202620, Israel, Ramat Hasharon, 4704151, Israel. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - J Phys Condens Matter JT - Journal of physics. Condensed matter : an Institute of Physics journal JID - 101165248 SB - IM OTO - NOTNLM OT - Fractional quantum Hall effect OT - Non-Abelian anyons OT - cavity quantum electrodynamics OT - electrostatic braiding OT - interferometric readout OT - quasihole pinning EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:03 PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:03 [entrez] AID - 10.1088/1361-648X/aebb85 [doi] PST - aheadofprint SO - J Phys Condens Matter. 2026 Oct 29. doi: 10.1088/1361-648X/aebb85. PMID- 42956463 OWN - NLM STAT- Publisher LR - 20261029 IS - 1748-3190 (Electronic) IS - 1748-3182 (Linking) DP - 2026 Oct 29 TI - Design and Analysis of a Bending-Torsion Coupled Bio-Inspired Claw Attachment Mechanism for Variable-Diameter Curved Surfaces. LID - 10.1088/1748-3190/aebb84 [doi] AB - Reliable aerial perching on variable-diameter curved surfaces requires both sufficient grasping preload and conformal interfacial contact, whereas conventional underactuated bird-claw-inspired mechanisms primarily accommodate in-plane geometric variations and therefore have limited adaptability to surfaces with spatially varying curvature. This study presents a bending-torsion coupled bird-claw-inspired attachment mechanism for variable-diameter curved surfaces. The key design combines direction-selective compliant joints with a bilateral force-balanced tendon transmission, allowing asymmetric contact reactions to passively reorient individual claw segments. This passive reorientation improves interfacial conformity during grasping and provides more favorable contact and sealing conditions for dry adhesion and vacuum suction. A grasping-stability model was employed to optimize the key structural parameters, followed by mechanical characterization of the compliant joints and attachment tests on smooth acrylic cylinders with diameters of 60-120 mm and cones with apex angles of 20 degrees -60 degrees . The equivalent bending stiffness of the compliant joint was tunable from 0.2 to 1.0 N.mm/ degrees , while high torsional compliance was maintained within the small-angle working range. The maximum circumferential attachment moment reached 1.65 N.m on a 120 mm diameter cylinder and 0.60 N.m on a 60 degrees apex-angle cone. A manually controlled flight experiment further demonstrated successful perching on a 20 degrees conical surface. These results demonstrate that bending-torsion coupled compliance can broaden the geometric adaptation range of underactuated claw-based attachment mechanisms and provide a feasible structural approach for aerial perching on variable-diameter curved surfaces. CI - (c) 2026 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved. FAU - Wang, Zhouyi AU - Wang Z AUID- ORCID: 0000-0002-4842-3470 AD - College of Mechanical and Electrical Engineering, Nanjing University of Aeronautics and Astronautics, No. 29, Yudao Street, Nanjing, Jiangsu, 210016, China. FAU - Zhao, Wei AU - Zhao W AD - College of Mechanical and Electrical Engineering, Nanjing University of Aeronautics and Astronautics, No. 29, Yudao Street, Nanjing, Jiangsu, 210016, China. FAU - Zhang, Chaofu AU - Zhang C AUID- ORCID: 0009-0001-9378-3870 AD - College of Mechanical and Electrical Engineering, Nanjing University of Aeronautics and Astronautics, No. 29, Yudao Street, Nanjing, Jiangsu, 210016, China. FAU - Wang, Liuwei AU - Wang L AD - School of Mechanical Engineering, Nanjing University of Industry Technology, No. 1, Yangshan North Road, Xianlin University Town, Qixia District, Nanjing, 210023, China. FAU - Li, Weiming AU - Li W AD - College of Mechanical and Electrical Engineering, Nanjing University of Aeronautics and Astronautics, No. 29, Yudao Street, Nanjing, Jiangsu, 210016, China. FAU - Ji, Aihong AU - Ji A AUID- ORCID: 0000-0002-1905-0564 AD - College of Mechanical and Electrical Engineering, Nanjing University of Aeronautics and Astronautics, No. 29, Yudao Street, Nanjing, Jiangsu, 210016, China. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Bioinspir Biomim JT - Bioinspiration & biomimetics JID - 101292902 SB - IM OTO - NOTNLM OT - aerial perching; OT - bending-torsion coupled compliance OT - bio-inspired avian claw OT - irregular curved surfaces OT - multi-mechanism attachment EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:02 PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:02 [entrez] AID - 10.1088/1748-3190/aebb84 [doi] PST - aheadofprint SO - Bioinspir Biomim. 2026 Oct 29. doi: 10.1088/1748-3190/aebb84. PMID- 42956448 OWN - NLM STAT- Publisher LR - 20261029 IS - 1521-0103 (Electronic) IS - 0022-3565 (Linking) VI - 393 IP - 10 DP - 2026 Sep 7 TI - Bioactivatable conjugated nanocarriers for synergistic mRNA-mediated cancer immunotherapy. PG - 105029 LID - S0022-3565(26)01229-2 [pii] LID - 10.1016/j.jpet.2026.105029 [doi] AB - mRNA immunotherapy holds transformative potential in oncology, yet single-agent approaches frequently fail against immunosuppressive tumor microenvironments (TMEs). Bioactivatable co-delivery of immunogenic chemotherapy alongside mRNA offers mechanistically synergistic advantages, yet coformulation of hydrophobic small-molecule prodrugs with hydrophilic mRNA in a single stable nanoparticle remains a major unresolved engineering challenge. We synthesized an activatable lipid-prodrug by covalently tethering an hydrogen peroxide-responsive thioketal-oxaliplatin conjugate to dilinoleylmethyl-4-dimethylaminobutyrate via amide coupling, and formulated bioactivatable conjugated nanocarriers (BCNs) by microfluidic nanoprecipitation. Four formulations were comprehensively characterized and evaluated for mRNA expression, cellular uptake, endosomal escape, immune activation, cytotoxicity, and in vivo antitumor efficacy, TME remodeling, immune memory, and systemic safety. BCN-D (combination) exhibited a hydrodynamic diameter of 112 +/- 3.5 nm (polydispersity index = 0.09), mRNA encapsulation efficiency of 91.5% +/- 1.6%, reactive oxygen species -triggered drug release of 88% in tumor homogenate versus <12% in PBS, and 60-day colloidal stability. BCN-D was associated with increased expression of cGAS- stimulator of interferon genes pathway markers and prominent ICD hallmarks (calreticulin: 7.8-fold; HMGB1: 7.1-fold), Th1-skewed cytokine secretion (IFN-gamma: 8.9-fold; IL-2: 11.3-fold), and a selectivity index (SI) >100 vs normal cells. In the B16-F10 syngeneic model, BCN-D achieved 84.7% tumor growth inhibition (combination index = 0.62), significantly prolonged survival (median not reached at day 40), and 100% durable tumor rejection upon rechallenge in complete responders. Tumor-infiltrating CD8(+)/Treg ratio improved 23-fold versus vehicle. Together, these findings characterize BCN-D as a combination nanocarrier in this preclinical model, with observed associations among ROS-responsive prodrug release, immune-marker changes, TME remodeling, and antitumor activity. These findings support further mechanistic investigation and evaluation in additional preclinical models before conclusions regarding translational applicability can be drawn. SIGNIFICANCE STATEMENT: This study presents bioactivatable conjugated nanocarriers combining reactive oxygen species-responsive oxaliplatin prodrug activation with mRNA immunotherapy, associated with altered tumor immune composition and antitumor activity in tested models. Building on prodrug-tethered ionizable lipid nanoparticles, it provides an incremental extension through thioketal-phenylboronic acid linker chemistry, an oxaliplatin prodrug designed to induce immunogenic cell death, and evaluation in melanoma. Translational relevance requires validation in additional preclinical systems. CI - Copyright (c) 2026 American Society for Pharmacology and Experimental Therapeutics. Published by Elsevier Inc. All rights reserved. FAU - Nazir, Noman AU - Nazir N AD - Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, Pakistan. Electronic address: nomikhan9637@gmail.com. FAU - Gajjar, Tushar B AU - Gajjar TB AD - Faculty of Pharmacy, Gokul Global University, Sidhpur, Gujarat, India. FAU - Hanumanthayya, Malathi AU - Hanumanthayya M AD - Department of Biotechnology and Genetics, School of Sciences, JAIN (Deemed to be University), Bangalore, Karnataka, India. FAU - Bainsal, Neeraj AU - Bainsal N AD - University Institute of Pharma Sciences, Chandigarh University, Mohali, Punjab, India. FAU - Singhal, Divya AU - Singhal D AD - Centre for Research Impact & Outcome, Chitkara College of Pharmacy, Chitkara University, Rajpura, Punjab, India; Sharda School of Bio-Science & Technology, Sharda University, Greater Noida, Uttar Pradesh, India. FAU - Smerat, Aseel AU - Smerat A AD - Faculty of Educational Sciences, Al-Ahliyya Amman University, Amman, Jordan. FAU - Alsharif, Khalaf F AU - Alsharif KF AD - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, Taif, Saudi Arabia. FAU - Alzahrani, Fuad M AU - Alzahrani FM AD - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, Taif, Saudi Arabia. FAU - Alzahrani, Khalid J AU - Alzahrani KJ AD - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, Taif, Saudi Arabia. FAU - Singh, Shekhar AU - Singh S AD - Department of Cardiology, Central Laboratory of The Lishui Hospital of Wenzhou Medical University, The First Affiliated Hospital of Lishui University, Lishui People's Hospital, Lishui, Zhejiang, China. Electronic address: drshekharsingh@126.com. LA - eng PT - Journal Article DEP - 20260907 PL - Netherlands TA - J Pharmacol Exp Ther JT - The Journal of pharmacology and experimental therapeutics JID - 0376362 SB - IM OTO - NOTNLM OT - Immunogenic cell death OT - Lipid nanoparticles OT - ROS-responsive prodrug OT - Tumor microenvironment OT - cGAS-STING OT - mRNA cancer vaccine COIS- Conflict of interest The authors declare no conflicts of interest. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:01 PHST- 2026/05/05 00:00 [received] PHST- 2026/08/30 00:00 [revised] PHST- 2026/09/01 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:01 [entrez] AID - S0022-3565(26)01229-2 [pii] AID - 10.1016/j.jpet.2026.105029 [doi] PST - aheadofprint SO - J Pharmacol Exp Ther. 2026 Sep 7;393(10):105029. doi: 10.1016/j.jpet.2026.105029. PMID- 42956446 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-4076 (Electronic) IS - 1879-4068 (Linking) VI - 18 IP - 2 DP - 2026 Oct 29 TI - Managing patients with advanced non-small cell lung cancer alongside complex comorbidities and frailty: A consensus statement and implications for the United Kingdom health system. PG - 104651 LID - S1879-4068(26)01808-4 [pii] LID - 10.1016/j.jgo.2026.104651 [doi] AB - Non-small cell lung cancer (NSCLC) is predominantly diagnosed in older adults, many of whom present with multiple comorbidities and varying degrees of frailty. These factors complicate diagnostic investigations, treatment decisions, and outcomes. Limited real-world guidance exists on managing advanced NSCLC in patients with comorbidities and frailty. To address this knowledge gap, a multidisciplinary panel of 10 United Kingdom (UK) experts, including six lung oncologists, two clinical nurse specialists, and two pharmacists, participated in two virtual workshops to identify key challenges and best practices for managing patients with advanced NSCLC and complex comorbidities or frailty. Twenty-three draft consensus statements were developed based on discussion at the workshops, with a further two experts (one respiratory physician and one geriatrician) invited to draft and/or review the statements at that time. The consensus statements were distributed via Microsoft Forms for anonymous voting and commentary. A predefined threshold of >/=70% agreement was set to define consensus. Comments not meeting this threshold were revised following expert review to increase agreement. Consensus was achieved for 22 of 23 statements. This consensus statement provides recommendations on assessments and care planning, prehabilitation strategies and comorbidity optimization, and treatment selection and individualization. The multidisciplinary, practical guidance for managing patients with advanced NSCLC with comorbidities and frailty also identifies evidence gaps requiring further study. Implementation of these recommendations may improve equitable, patient-centered care within the UK National Health Service and could be applicable to other healthcare systems. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Gomes, Fabio AU - Gomes F AD - The Christie NHS Foundation Trust, Manchester, UK. Electronic address: fabio.gomes2@nhs.net. FAU - Talbot, Toby AU - Talbot T AD - Royal Cornwall Hospital Treliske, Truro, UK. FAU - Clayton, Karen AU - Clayton K AD - Macclesfield District General Hospital, Macclesfield, UK. FAU - Baijal, Shobhit AU - Baijal S AD - University Hospitals Birmingham NHS Trust, Birmingham, UK. FAU - Chitnis, Meenali AU - Chitnis M AD - Oxford University Hospitals NHS Foundation Trust, Oxford, UK. FAU - Kalsi, Tania AU - Kalsi T AD - Guy's and St Thomas' NHS Foundation Trust, London, UK; CARICE, Faculty of Life Sciences & Medicine, King's College London, London, UK. FAU - Navani, Neal AU - Navani N AD - University College Hospital, London, UK. FAU - Fenemore, Jackie AU - Fenemore J AD - The Christie NHS Foundation Trust, Manchester, UK. FAU - Pearce, Mark AU - Pearce M AD - The Christie NHS Foundation Trust, Manchester, UK. FAU - Ibrahimo, Salma AU - Ibrahimo S AD - Regeneron UK Ltd., Uxbridge, UK. FAU - Schenk, Max AU - Schenk M AD - Regeneron UK Ltd., Uxbridge, UK. FAU - Greystoke, Alastair AU - Greystoke A AD - Newcastle upon Tyne Hospitals NHS Foundation Trust, Newcastle upon Tyne, UK. FAU - Slavova-Boneva, Vanya AU - Slavova-Boneva V AD - Royal Marsden NHS Foundation Trust, London, UK. FAU - Escriu, Carles AU - Escriu C AD - The Clatterbridge Cancer Centre NHS Foundation Trust, Liverpool, UK. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - Netherlands TA - J Geriatr Oncol JT - Journal of geriatric oncology JID - 101534770 SB - IM OTO - NOTNLM OT - Advanced non-small cell lung cancer OT - Comorbidities OT - Consensus OT - Frailty OT - Multidisciplinary care OT - UK EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:01 PHST- 2026/05/27 00:00 [received] PHST- 2026/09/04 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:01 [entrez] AID - S1879-4068(26)01808-4 [pii] AID - 10.1016/j.jgo.2026.104651 [doi] PST - aheadofprint SO - J Geriatr Oncol. 2026 Oct 29;18(2):104651. doi: 10.1016/j.jgo.2026.104651. PMID- 42956432 OWN - NLM STAT- Publisher LR - 20261029 IS - 1751-732X (Electronic) IS - 1751-7311 (Linking) VI - 20 IP - 11 DP - 2026 Oct 1 TI - Health and welfare of dairy cows in barn- versus pasture-based systems. PG - 101971 LID - S1751-7311(26)00218-1 [pii] LID - 10.1016/j.animal.2026.101971 [doi] AB - The aim of the present study was to assess animal welfare in two distinct dairy systems (barn- vs pasture-based) in Switzerland over an entire year. All selected farms operated loose-housing systems and dairy herds had pasture access for at least half of the year. In general, barn-based (BB) systems focus on milk production per cow with a feeding strategy mainly based on a mixed ration of conserved feed and concentrates, whereas pasture-based (PB) systems focus on milk production per hectare of pastureland and a feeding strategy that optimises the use of fresh and conserved grass. Fifteen farms from each system were visited four times during the year to assess clinical health and behavioural indicators. Statistical analyses were conducted using binomial generalised linear mixed-effects models at herd-level to identify differences between systems and visits within a system. One third of assessed clinical health indicators showed a prevalence above 1%. Over all visits, hairless patches (17 vs 8%), lesions (6 vs 1%), hindleg swellings (1 vs 0%), too lean body condition score (30 vs 22%), soiled udders (13 vs 9%), stiff walking (27 vs 7%) and lameness (11 vs 1%) were more prevalent in BB herds than in PB herds. During the pasture period, the prevalence of hairless patches and lesions was lower than during the housing period, while soiled hindquarters declined in BB herds but increased in PB herds. No difference in the prevalence of lameness was found between the pasture and housing period; however, the prevalence of overgrown hooves was lower during the pasture period for both systems. Two-thirds of the cows in both systems had an acceptable body condition score, with no differences found over the four visits. The two systems revealed distinct morning activity patterns, with a higher percentage of BB cows lying down and a lower percentage eating compared to PB cows. Observed social behaviours occurred at low frequencies, with allogrooming being the most frequently recorded behaviour in both systems. The ability to closely approach half of the cows in both systems suggests a positive human-animal relationship, despite PB herds having more pasture access than BB herds. The herd-level respiration rate and Temperature-Humidity-Index revealed a weak-to-moderate relationship across both systems (R(2) = 0.36 for BB and R(2) = 0.25 for PB). Results from the present study demonstrate that both dairy systems achieved relatively high animal welfare standards, with relatively minor differences observed between the two contrasting systems. CI - Copyright (c) 2026 The Author(s). Published by Elsevier B.V. All rights reserved. FAU - Braun, J AU - Braun J AD - School of Agricultural, Forest and Food Sciences HAFL, Bern University of Applied Sciences, Langgasse 85, Zollikofen, 3052, Switzerland. FAU - Birkinshaw, A AU - Birkinshaw A AD - School of Agricultural, Forest and Food Sciences HAFL, Bern University of Applied Sciences, Langgasse 85, Zollikofen, 3052, Switzerland. FAU - Riedel, J AU - Riedel J AD - Institut fur Agrarokologie, Vordere Vorstadt 29, Aarau, 5000, Switzerland. FAU - Reidy, B AU - Reidy B AD - School of Agricultural, Forest and Food Sciences HAFL, Bern University of Applied Sciences, Langgasse 85, Zollikofen, 3052, Switzerland. FAU - Bokkers, E A M AU - Bokkers EAM AD - Animal Productions System Group, Wageningen University & Research, P.O. Box 338, Wageningen, 6700AH, the Netherlands. Electronic address: eddie.bokkers@wur.nl. LA - eng PT - Journal Article DEP - 20261001 PL - England TA - Animal JT - Animal : an international journal of animal bioscience JID - 101303270 SB - IM OTO - NOTNLM OT - Animal-based OT - Dairy cattle OT - Grazing OT - Heat stress OT - Social behaviour EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:00 PHST- 2025/12/31 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:00 [entrez] AID - S1751-7311(26)00218-1 [pii] AID - 10.1016/j.animal.2026.101971 [doi] PST - aheadofprint SO - Animal. 2026 Oct 1;20(11):101971. doi: 10.1016/j.animal.2026.101971. PMID- 42956431 OWN - NLM STAT- Publisher LR - 20261029 IS - 1751-732X (Electronic) IS - 1751-7311 (Linking) VI - 20 IP - 11 DP - 2026 Sep 25 TI - Identification of non-invasive indicators in saliva for LPS-induced inflammation in pigs fed a high-fibre or low-fibre diet. PG - 101966 LID - S1751-7311(26)00213-2 [pii] LID - 10.1016/j.animal.2026.101966 [doi] AB - Non-invasive biomarkers are increasingly used to detect pathological conditions in pigs without compromising animal welfare. Although saliva is considered a promising diagnostic matrix, it is unclear whether systemic inflammation can be reliably detected. This study aimed to characterise the response to lipopolysaccharide (LPS) challenge in pigs fed different dietary fibre contents and identify diet-independent indicators of inflammation in saliva. Male German Landrace littermate pigs were fed either a low-fibre (LF, 2.8%) or high-fibre (HF, 6.5%) diet from 50 days of age. At 75 days, pigs were intramuscularly injected with either LPS (30 microg/kg BW) or NaCl solution as a control, resulting in four groups (HF-LPS, HF-NaCl, LF-LPS, LF-NaCl; n = 9-10/group). Rectal temperature (RT) was recorded, and blood and saliva samples were collected at 0, 2, 4, 6 and 12 h postinjection. Plasma and saliva free amino acids and amino acid metabolites were determined by HPLC, and biochemical parameters were measured by an automatic enzymatic analyser. An automatic haematology analyser was used to measure haematological parameters. Salivary cortisol and plasma cytokines were measured by immunoassays. Multivariate integration analysis was performed using the Data Integration Analysis for Biomarker discovery using Latent cOmponents (DIABLO) framework (mixOmics package, R), followed by linear mixed-effects models to evaluate time-dependent changes. The HF and LF diets resulted in different basal metabolic profiles, but did not alter the inflammatory response to LPS, including typical acute-phase responses such as elevated RT, increased salivary cortisol, cytokines, lactate and bilirubin levels in plasma, and reduced concentrations of circulating amino acids and leucocytes. The salivary responses were less pronounced than those in plasma, but showed time-dependent changes. The DIABLO analysis consistently identified salivary 5-Aminovaleric acid (5AVA), a microbial lysine metabolite, as the only parameter distinguishing LPS pigs from controls at all time points after exposure, regardless of diet. Linear mixed-effects models confirmed higher 5AVA concentrations post-LPS compared to baseline. In summary, the inflammation induced by LPS in pigs was reflected in metabolic alterations of plasma and saliva. Salivary 5AVA showed potential as a non-invasive indicator of inflammation in pigs, as its post-LPS response was consistent and demonstrated a diet-independent association with the inflammatory response. However, further studies are required to validate its diagnostic performance under various inflammatory and dietary conditions. CI - Copyright (c) 2026 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Cavalleri, M AU - Cavalleri M AD - Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany. FAU - Sciascia, M AU - Sciascia M AD - Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany. FAU - Gors, S AU - Gors S AD - Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany. FAU - Vernunft, A AU - Vernunft A AD - Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany. FAU - Reyer, H AU - Reyer H AD - Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany. FAU - Oster, M AU - Oster M AD - Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany. FAU - Moscovice, L R AU - Moscovice LR AD - Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany. FAU - Wimmers, K AU - Wimmers K AD - Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany; University of Rostock, Faculty of Agriculture, Civil and Environmental Engineering, 18059 Rostock, Germany. FAU - Zentek, J AU - Zentek J AD - Freie Universitat Berlin, Institute of Animal Nutrition, 14195 Berlin, Germany. FAU - Danicke, S AU - Danicke S AD - Federal Research Institute for Animal Health, Institute of Animal Nutrition, 38116 Braunschweig, Germany. FAU - Kersten, S AU - Kersten S AD - Federal Research Institute for Animal Health, Institute of Animal Nutrition, 38116 Braunschweig, Germany. FAU - Van Rymenant, E AU - Van Rymenant E AD - Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), 9820 Merelbeke-Melle, Belgium. FAU - Metges, C C AU - Metges CC AD - Research Institute for Farm Animal Biology (FBN), 18196 Dummerstorf, Germany; University of Rostock, Faculty of Agriculture, Civil and Environmental Engineering, 18059 Rostock, Germany. Electronic address: metges@fbn-dummerstorf.de. LA - eng PT - Journal Article DEP - 20260925 PL - England TA - Animal JT - Animal : an international journal of animal bioscience JID - 101303270 SB - IM OTO - NOTNLM OT - 5-Aminovaleric Acid OT - Amino acids OT - Biomarker OT - Metabolites OT - Multivariate analysis EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:00 PHST- 2026/04/14 00:00 [received] PHST- 2026/09/13 00:00 [revised] PHST- 2026/09/14 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:00 [entrez] AID - S1751-7311(26)00213-2 [pii] AID - 10.1016/j.animal.2026.101966 [doi] PST - aheadofprint SO - Animal. 2026 Sep 25;20(11):101966. doi: 10.1016/j.animal.2026.101966. PMID- 42956429 OWN - NLM STAT- Publisher LR - 20261029 IS - 1532-7361 (Electronic) IS - 0039-6060 (Linking) VI - 202 DP - 2026 Sep 26 TI - Incidence, timing, severity, and predictors of venous thromboembolism after open abdominal wall reconstruction. PG - 110712 LID - S0039-6060(26)00637-9 [pii] LID - 10.1016/j.surg.2026.110712 [doi] AB - BACKGROUND: Venous thromboembolic events, including deep vein thrombosis and pulmonary embolism, represent serious and potentially preventable complications following open abdominal wall reconstruction. This study aimed to determine the incidence and predictors of venous thromboembolic events after open abdominal wall reconstruction. METHODS: A single-institution prospectively maintained database was queried for open abdominal wall reconstruction (2010-2025). Standard statistical analyses and multivariable regression analyses were performed to identify predictors of venous thromboembolic events. RESULTS: Among 2,907 open abdominal wall reconstructions, 49 venous thromboembolic events (1.7%) occurred: 31 pulmonary embolisms +/- deep vein thrombosis and 18 deep vein thromboses alone. Average body mass index was 34.6 +/- 10.0 kg/m(2), and 18.4% had prior venous thromboembolic events. Preoperative chemoprophylaxis was administered in 77.6% of venous thromboembolic event cases, and 93.8% of patients who received postoperative chemoprophylaxis had no interruption. Compared with patients without venous thromboembolic events, those with venous thromboembolic events had larger defect sizes (314.5 +/- 222.7 vs 199.8 +/- 187.5 cm(2); P < .001) and longer operative times (233.4 +/- 74.9 vs 187.2 +/- 84.4 minutes; P < .001). Among 29 classified pulmonary embolisms, 13 were nonmassive, 12 submassive, and 4 massive/fatal. Advanced interventions were required in 12.2%, 16.3% required transfusion, and 16.3% underwent inferior vena cava filter placement. Among venous thromboembolic event patients, 40.8% required intensive care unit transfer, with 20.4% requiring reintubation. Average length of stay was longer in venous thromboembolic event patients (11.4 +/- 9.8 vs 5.7 +/- 8.8 days; P < .001). Multivariable regression identified body mass index (odds ratio, 1.09; 95% confidence interval, 1.04, 1.14; P < .001), prior venous thromboembolic events (odds ratio, 4.51; 95% confidence interval, 1.91, 10.62; P < .001), and operative time (odds ratio, 1.005; 95% confidence interval, 1.001, 1.010; P = .008) as independent predictors of venous thromboembolic event. CONCLUSION: Postoperative venous thromboembolic event following open abdominal wall reconstruction is uncommon but associated with significant morbidity and mortality. Higher body mass index, prior venous thromboembolic event, and longer operative time independently predicted venous thromboembolic event, highlighting the need for improved risk stratification and prevention strategies. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Kerr, Samantha W AU - Kerr SW AD - Division of Gastrointestinal and Minimally Invasive Surgery, Department of Surgery, Carolinas Medical Center, Charlotte, NC. FAU - Hinton, Lucy R AU - Hinton LR AD - Division of Gastrointestinal and General Surgery, Department of Surgery, Endeavor Health, Evanston, IL. FAU - Tawkaliyar, Rahmatulla AU - Tawkaliyar R AD - Division of Gastrointestinal and Minimally Invasive Surgery, Department of Surgery, Carolinas Medical Center, Charlotte, NC. FAU - Scarola, Gregory T AU - Scarola GT AD - Division of Gastrointestinal and Minimally Invasive Surgery, Department of Surgery, Carolinas Medical Center, Charlotte, NC. FAU - Ayuso, Sullivan A AU - Ayuso SA AD - Division of Elective General Surgery, Department of Surgery and Perioperative Care, University of Texas, Austin, TX. FAU - Sing, Ronald F AU - Sing RF AD - Division of Acute Care Surgery, Department of Surgery, Carolinas Medical Center, Charlotte, NC. FAU - Kercher, Kent W AU - Kercher KW AD - Division of Gastrointestinal and Minimally Invasive Surgery, Department of Surgery, Carolinas Medical Center, Charlotte, NC. FAU - Heniford, B Todd AU - Heniford BT AD - Division of Gastrointestinal and General Surgery, Department of Surgery, Endeavor Health, Evanston, IL. Electronic address: https://x.com/THeniford. FAU - Augenstein, Vedra A AU - Augenstein VA AD - Department of Surgery, Novant Health Presbyterian, Charlotte, NC. Electronic address: Vedra.augenstein@gmail.com. LA - eng PT - Journal Article DEP - 20260926 PL - United States TA - Surgery JT - Surgery JID - 0417347 SB - IM COIS- Conflict of Interest/Disclosure Dr Augenstein receives speaking honoraria from Allergan, Pacira, and Medtronic. Dr Heniford receives surgical and research education grants and honoraria from W.L. Gore and Medtronic, an educational grant from Intuitive, and a research grant from Merck. Dr Kercher is a consultant for Medtronic. Dr Ayuso receives education funding, is a speaker, and consultant for Boston Scientific. Dr Kerr, Dr Hinton, Dr Sing, Mr. Tawkaliyar, and Mr. Scarola have no disclosures to report. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 19:00 PHST- 2026/06/15 00:00 [received] PHST- 2026/07/30 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 19:00 [entrez] AID - S0039-6060(26)00637-9 [pii] AID - 10.1016/j.surg.2026.110712 [doi] PST - aheadofprint SO - Surgery. 2026 Sep 26;202:110712. doi: 10.1016/j.surg.2026.110712. PMID- 42956421 OWN - NLM STAT- Publisher LR - 20261029 IS - 0325-7541 (Print) IS - 0325-7541 (Linking) VI - 59 IP - 1 DP - 2026 Oct 29 TI - Surveillance of high-priority enterococci and factors associated with clinical enterococcal infections in a Reference Hospital in northern Nigeria. PG - 100761 LID - S0325-7541(26)00071-4 [pii] LID - 10.1016/j.ram.2026.100761 [doi] AB - Vancomycin-resistant enterococci (VRE) are high-priority pathogens; however, most healthcare providers in resource-limited settings do not consider enterococci in the differential diagnosis of clinical bacterial infections. This cross-sectional surveillance study determined the antimicrobial resistance (AMR) profile of Enterococcus and factors associated with their presence in patients attending the Ahmadu Bello University Teaching Hospital (ABUTH), Zaria, Nigeria. Non-repetitive clinical samples from 180 patients were processed for the isolation, identification, and antimicrobial susceptibility testing of enterococci in Slanetz-Bartley agar and the Vitek(R) 2 Compact system. Structured questionnaires were used to collect participants' sociodemographic and risk factor data. The prevalence of enterococcal infection was 23.3% (42/180), comprising 57.1% Enterococcus faecalis and 42.9% Enterococcus faecium. Subgroup analysis showed no significant association between the isolation rates and sample type (p=0.379). The frequency of enterococcal infection was significantly highest among patients aged 31-40 years (p<0.05), with a higher frequency among older patients. Moreover, unemployed patients (OR=1.8, p=0.0195) and those using pit latrines (OR=2.1, p=0.034) had significantly increased the odds of enterococcal infection. Enterococci resistance was highest against ampicillin (100%), tetracycline (90.5%), and ciprofloxacin (69%), with 83.3% exhibiting a multidrug-resistant (MDR) phenotype. Remarkably, 7.1% of all isolates were VR-E. faecium. Moreover, enterococci with a multiple antimicrobial resistance (MAR) index of 0.3 (45.2%) were predominant. A high level of MDR enterococci (including VRE) was identified in the clinical samples from patients initially managed for non-enterococcal infections, underscoring the need to use a selective bacteriological medium to minimize microbiological misdiagnosis in resource-limited healthcare settings. This study provides valuable local surveillance data indicating gaps in the integration of appropriate diagnostic bacteriology into clinical infectious disease practice. CI - Copyright (c) 2026 The Author(s). Publicado por Elsevier Espana, S.L.U. All rights reserved. FAU - Muhammad Auwal, Aisha AU - Muhammad Auwal A AD - Department of Medical Laboratory Science, Faculty of Allied Health Sciences, College of Medical Sciences, Ahmadu Bello University, Zaria, Nigeria. FAU - Nasir Abdullahi, Idris AU - Nasir Abdullahi I AD - Department of Medical Laboratory Science, Faculty of Allied Health Sciences, College of Medical Sciences, Ahmadu Bello University, Zaria, Nigeria. Electronic address: eedris888@yahoo.com. FAU - Trabelsi, Islem AU - Trabelsi I AD - Bioresources, Environment and Biotechnology Laboratory (LR22ES04), Higher Institute of Applied Biological Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia. FAU - Almustapha, Shamsu AU - Almustapha S AD - Department of Medical Laboratory Science, Faculty of Allied Health Sciences, College of Medical Sciences, Ahmadu Bello University, Zaria, Nigeria. FAU - Muhammad, Attahiru AU - Muhammad A AD - ART Laboratory, Ahmadu Bello University Teaching Hospital, Zaria, Nigeria. FAU - Abdurrazaq, Shamsuddeen AU - Abdurrazaq S AD - Department of Medical Microbiology, Ahmadu Bello University Teaching Hospital, Zaria, Nigeria. FAU - Mohammed, Musa AU - Mohammed M AD - Bioresources, Environment and Biotechnology Laboratory (LR22ES04), Higher Institute of Applied Biological Sciences of Tunis, University of Tunis El Manar, Tunis, Tunisia. FAU - Umar, Kabir AU - Umar K AD - Department of Medical Laboratory Science, Faculty of Allied Health Sciences, College of Medical Sciences, Ahmadu Bello University, Zaria, Nigeria. LA - eng PT - Journal Article DEP - 20261029 PL - Argentina TA - Rev Argent Microbiol JT - Revista Argentina de microbiologia JID - 8002834 SB - IM OTO - NOTNLM OT - Antimicrobial resistance OT - Enterococcal infections OT - Enterococos resistentes a la vancomicina OT - Healthcare-associated enterococci OT - Infecciones por enterococos OT - Nigeria OT - Resistencia antimicrobiana OT - Vancomycin-resistant enterococci OT - enterococos asociados a la atencion sanitaria EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 18:59 PHST- 2026/05/14 00:00 [received] PHST- 2026/07/13 00:00 [revised] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 18:59 [entrez] AID - S0325-7541(26)00071-4 [pii] AID - 10.1016/j.ram.2026.100761 [doi] PST - aheadofprint SO - Rev Argent Microbiol. 2026 Oct 29;59(1):100761. doi: 10.1016/j.ram.2026.100761. PMID- 42956420 OWN - NLM STAT- Publisher LR - 20261029 IS - 1878-1799 (Electronic) IS - 1871-5192 (Linking) VI - 39 IP - 6 DP - 2026 Oct 29 TI - Adolescent mothers' lived experiences of respectful and non-respectful maternity care: A systematic review and qualitative evidence synthesis. PG - 102412 LID - S1871-5192(26)00255-6 [pii] LID - 10.1016/j.wombi.2026.102412 [doi] AB - BACKGROUND: Adolescent mothers are particularly vulnerable to experiencing disrespectful maternity care because of their young age and social vulnerability. AIM: This systematic review and qualitative evidence synthesis aimed to synthesise and interpret qualitative evidence on adolescent mothers' perceptions and experiences of respectful and disrespectful maternity care. METHODS: This study was conducted as a systematic review and qualitative evidence synthesis. A systematic search was performed in PubMed, Scopus and Web of Science to identify articles published between 2010 and 2026. Qualitative studies reporting adolescent mothers' experiences of prenatal, childbirth, or postpartum care were included. Study screening and selection are presented using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) flow chart. The methodological quality of the included studies was appraised using the Critical Appraisal Skills Programme (CASP) checklist. Data were analysed using the thematic synthesis approach described by Thomas and Harden (2008). FINDINGS: A total of 22 qualitative studies were included. Data analysis resulted in the identification of five analytical themes: Adolescence as a socially discrediting identity in maternity care, Struggling for voice, autonomy, and recognition, Violations of dignity, privacy, and emotional safety, Negotiating maternity care within unsupportive health systems, and Respectful relationships as pathways to trust, confidence, and empowerment. CONCLUSION: Respectful maternity care experiences among adolescent mothers are shaped by the interplay of social stigma, caregiving relationships, and health system structures. These findings highlight the need for adolescent-responsive health systems that address stigma, uphold adolescents' dignity, autonomy, and participation, and remove structural barriers to equitable maternity care. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Yas, Atefeh AU - Yas A AD - Reproductive Health Research Center, Clinical Research Institute, Urmia University of Medical Sciences, Urmia, Iran. Electronic address: Atefehyas@yahoo.com. FAU - Rabeipoor, Soheila AU - Rabeipoor S AD - Reproductive Health Research Center, Clinical Research Institute, Urmia University of Medical Sciences, Urmia, Iran. Electronic address: Soheila80@yahoo.com. FAU - Etesami, Ms Elmira AU - Etesami ME AD - Nursing and Midwifery School, Urmia University of Medical Sciences, Urmia, Iran. Electronic address: Etesami258@gmail.com. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - Netherlands TA - Women Birth JT - Women and birth : journal of the Australian College of Midwives JID - 101266131 SB - IM OTO - NOTNLM OT - Abuse OT - Adolescent Mothers OT - Experiences OT - Mistreatment OT - Qualitative evidence synthesis OT - Respectful Maternity Care OT - Stigma COIS- Declaration of Competing Interest The authors declare that they have no conflicts of interest. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 18:59 PHST- 2026/07/25 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 18:59 [entrez] AID - S1871-5192(26)00255-6 [pii] AID - 10.1016/j.wombi.2026.102412 [doi] PST - aheadofprint SO - Women Birth. 2026 Oct 29;39(6):102412. doi: 10.1016/j.wombi.2026.102412. PMID- 42956417 OWN - NLM STAT- Publisher LR - 20261029 IS - 2057-1976 (Electronic) IS - 2057-1976 (Linking) DP - 2026 Oct 29 TI - Monitoring and modeling of pharyngeal behavior during swallowing using bioelectrical impedance technique. LID - 10.1088/2057-1976/aebb33 [doi] AB - To perform a non-invasive swallowing test for patients suspected of having dysphagia, a system capable of monitoring pharyngeal behavior simply and easily during swallowing was considered. The system incorporating a bioelectrical impedance technique was assembled to input an alternating current into the neck of the subjects and to output the corresponding voltage response. We measured the voltages during swallowing using this system and observed the voltage changes for swallowing to evaluate the system validity. The relationship between the characteristic voltage changes and the movements of tissues or organs, specifically those of the hyoid bone, were investigated on the basis of the measurements at various positions in the neck region. The actions of the related muscles in the pharyngeal phase of swallowing were explained by a muscle group model described with an electrical circuit. Additionally, the voltage measured in swallowing was assessed as resistive and reactive components. This approach demonstrated a potential of this system to detect cellular-level changes. . CI - Creative Commons Attribution license. FAU - Yoshida, Masanori AU - Yoshida M AUID- ORCID: 0000-0002-1032-1014 AD - Applied Sciences, Muroran Institute of Technology, 27-1, Mizumotocho, Muroran, 050-8585, Japan. FAU - Nishibayashi, Kaito AU - Nishibayashi K AD - Applied Sciences, Muroran Institute of Technology, 27-1, Mizumotocho, Muroran, 050-8585, Japan. FAU - Uchida, Kyoka AU - Uchida K AD - Applied Sciences, Muroran Institute of Technology, 27-1, Mizumotocho, Muroran, 050-8585, Japan. FAU - Kawaguchi, Takuya AU - Kawaguchi T AD - Applied Sciences, Muroran Institute of Technology, 27-1, Mizumotocho, Muroran, 050-8585, Japan. FAU - Gawa, Taiga AU - Gawa T AD - Applied Sciences, Muroran Institute of Technology, 27-1, Mizumotocho, Muroran, 050-8585, Japan. FAU - Kanda, Kazuma AU - Kanda K AD - Applied Sciences, Muroran Institute of Technology, 27-1, Mizumotocho, Muroran, 050-8585, Japan. FAU - Sei, Hirofumi AU - Sei H AUID- ORCID: 0000-0002-1329-9115 AD - Otorhinolaryngology and Head and Neck Surgery, Ehime Prefectural Central Hospital, 83, Kasugamachi, Matsuyama, 790-0024, Japan. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Biomed Phys Eng Express JT - Biomedical physics & engineering express JID - 101675002 SB - IM OTO - NOTNLM OT - Bioelectrical impedance technique OT - Hyoid bone OT - Monitoring OT - Pharynx OT - Swallowing EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 18:53 PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 18:53 [entrez] AID - 10.1088/2057-1976/aebb33 [doi] PST - aheadofprint SO - Biomed Phys Eng Express. 2026 Oct 29. doi: 10.1088/2057-1976/aebb33. PMID- 42956416 OWN - NLM STAT- Publisher LR - 20261029 IS - 1741-2552 (Electronic) IS - 1741-2552 (Linking) DP - 2026 Oct 29 TI - Bipolar intracortical microstimulation expands the repertoire of neuronal activation patterns. LID - 10.1088/1741-2552/aebb67 [doi] AB - Intracortical microstimulation is a promising approach for sensory restoration, but its spatial selectivity and variability of human perception are constrained by current spread and electrode configuration. We investigated how varying the return-electrode position during intracortical bipolar stimulation influences the spatial distribution and composition of activated neuronal populations. Approach: We combined two-photon calcium imaging in acute mouse cortical slices with biophysically grounded computational modeling to characterize neuronal activation at the cellular and population level. We applied bipolar stimulation across a range of electrode configurations, systematically varying current direction, electrode spacing, and stimulation parameters. Main results: Bipolar stimulation activated distinct neuronal populations depending on electrode configuration, with activation patterns becoming less overlapping as the angular difference between stimulation directions increased. Population-level analyses revealed a small number of dominant activation patterns across stimulation configurations. Electrode spacing influenced both the number of activated neurons and the spatial extent of activation, whereas polarity and waveform asymmetry had comparatively modest effects. Computational modeling indicated that bipolar activation predominantly reflected the combined effect of the two corresponding monopolar stimulations, with an additional bipolar-specific component. Neurons activated exclusively by bipolar stimulation were relatively sparse and spatially dispersed. Significance: Our findings demonstrate that varying bipolar electrode configurations produces distinct patterns of neuronal activation, providing an additional mode for recruiting different neural populations. Computational modeling suggests that these patterns largely reflect the combined contributions of the individual electrodes, with additional differences which may reflect non-linear neuronal or network interactions. Together, these findings provide insight into how the electrode configuration shapes cortical activation patterns and how intracortical bipolar stimulation provides additional ways to activate neuronal populations without increasing electrode density. . CI - Creative Commons Attribution license. FAU - Schelles, Maarten AU - Schelles M AUID- ORCID: 0000-0003-3024-4869 AD - Electrical Engineering (ESAT), KU Leuven, Kasteelpark Arenberg 10, Leuven, 3001, Belgium. FAU - Verhaege, Anke AU - Verhaege A AUID- ORCID: 0009-0002-8086-7044 AD - ReVision Implant, Blandenstraat 31, Haasrode, 3053, Belgium. FAU - Van Rompaey, Nils AU - Van Rompaey N AD - ReVision Implant, Blandenstraat 31, 3053, Haasrode, Belgium. FAU - Goyvaerts, Laurens AU - Goyvaerts L AUID- ORCID: 0000-0002-2881-9291 AD - Electrical Engineering (ESAT), KU Leuven, Kasteelpark Arenberg 10, Leuven, 3001, Belgium. FAU - Wierda, Keimpe AU - Wierda K AUID- ORCID: 0000-0002-8784-9490 AD - Flemish Institute for Biotechnology (VIB), Herestraat 49, 3000, Leuven, Belgium. FAU - Ceyssens, Frederik AU - Ceyssens F AD - Revision Implant, Blandenstraat 31, Haasrode, 3053, Belgium. FAU - Kraft, Michael AU - Kraft M AUID- ORCID: 0000-0002-7213-1072 AD - KU Leuven, Kasteelpark Arenberg 10, Kasteelpark Arenberg 10, Leuven, 3000, Belgium. FAU - Bonin, Vincent AU - Bonin V AUID- ORCID: 0000-0002-9437-8092 AD - VIB-KU Leuven Center for Neuroscience Leuven, Herestraat 49, Leuven, 3000, Belgium. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - J Neural Eng JT - Journal of neural engineering JID - 101217933 SB - IM OTO - NOTNLM OT - Bipolar stimulation OT - current steering OT - intracortical microstimulation OT - neural prosthetics OT - two-photon calcium imaging OT - visual cortex EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 18:53 PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 18:53 [entrez] AID - 10.1088/1741-2552/aebb67 [doi] PST - aheadofprint SO - J Neural Eng. 2026 Oct 29. doi: 10.1088/1741-2552/aebb67. PMID- 42956415 OWN - NLM STAT- Publisher LR - 20261029 IS - 1741-2552 (Electronic) IS - 1741-2552 (Linking) DP - 2026 Oct 29 TI - Towards improving Alzheimer's intervention: a machine learning approach for biomarker detection through combining MEG and MRI pipelines. LID - 10.1088/1741-2552/aebb66 [doi] AB - OBJECTIVE: To identify robust biomarkers for early cognitive decline, classification performance was evaluated for healthy controls (HC) versus mild cognitive impairment (MCI) using MEG-derived spectral features at the sensor and source levels, and by testing whether adding MRI anatomical features improves discrimination in the BioFIND cohort. APPROACH: Data from 324 participants (166 HC and 158 MCI) were analyzed using automated artifact rejection and source reconstruction. Spectral biomarkers, including sensor-space and sourcespace representations, were extracted from the magnetometer and gradiometer signals. Five inverse-solution methods were assessed (including linearly constrained minimum variance [LCMV] and exact low-resolution electromagnetic tomography analysis [eLORETA]) to increase the robustness of source-based biomarker selection. Multiple classifiers, including a support vector machine (SVM) and L1-regularized logistic regression (GLMNET), were compared using nested K-fold cross-validation embedded in Monte Carlo replications to limit overfitting. MAIN RESULTS: The best performance was obtained by combining MRI features with source-based MEG features using GLMNET, achieving 76.31 +/- 1.47% accuracy and 82.44 +/- 1.44% AUC. MEG-only models showed strong discrimination and outperformed MRI-only analyses, particularly those based on the LCMV and eLORETA features. Across settings, the SVM and GLMNET were the most consistently effective classifiers. SIGNIFICANCE: These findings support MEG/EEG spectral biomarkers, particularly source-based representations, as sensitive markers of early AD-related functional changes. Integrating MEG with MRI can further improve HC-versus-MCI classification. The study also demonstrated that rigorous validation (nested cross-validation with Monte Carlo replication) is essential for reliable biomarker assessment and for comparing preprocessing, inverse-solution, and classifier choices in translational neuroimaging pipelines. CI - Creative Commons Attribution license. FAU - Ahmad, Alwani Liyana AU - Ahmad AL AD - Universiti Teknologi PETRONAS, Department of Applied Sciences, Seri Iskandar, 32610, Malaysia. FAU - Sanchez-Bornot, Jose Miguel AU - Sanchez-Bornot JM AUID- ORCID: 0000-0003-4014-4255 AD - Ulster University School of Computing Engineering and Intelligent Systems, Ulster University, Derry, Londonderry, BT48 7JL, United Kingdom of Great Britain and Northern Ireland. FAU - Sotero, Roberto C AU - Sotero RC AUID- ORCID: 0000-0001-9640-2722 AD - University of Calgary, Department of Radiology and Hotchkiss Brain Institute, Calgary, Alberta, T2N 1N4, Canada. FAU - Coyle, Damien H AU - Coyle DH AUID- ORCID: 0000-0002-4739-1040 AD - Department of Computer Science, University of Bath, Bath Institute for the Augmented Human, Bath, Bath, BA2 7AY, United Kingdom of Great Britain and Northern Ireland. FAU - Idris, Zamzuri AU - Idris Z AUID- ORCID: 0000-0002-9470-0571 AD - Hospital Pakar Universiti Sains Malaysia, Department of Neurosciences, Kota Bharu, Kelantan, 11800, Malaysia. FAU - Faye, Ibrahima AU - Faye I AD - Universiti Teknologi PETRONAS, Department of Applied Sciences, Seri Iskandar, 32610, Malaysia. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - J Neural Eng JT - Journal of neural engineering JID - 101217933 SB - IM OTO - NOTNLM OT - Alzheimer's disease OT - Brain source localisation OT - Classification analysis OT - GLMNET OT - MEG/EEG/MRI OT - multimodal integration OT - spectral features EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 18:53 PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 18:53 [entrez] AID - 10.1088/1741-2552/aebb66 [doi] PST - aheadofprint SO - J Neural Eng. 2026 Oct 29. doi: 10.1088/1741-2552/aebb66. PMID- 42956414 OWN - NLM STAT- Publisher LR - 20261029 IS - 1741-2552 (Electronic) IS - 1741-2552 (Linking) DP - 2026 Oct 29 TI - Spectral smoothing explains the offline gain from cross-order single-trial MEG movement decoding for non-invasive BCI. LID - 10.1088/1741-2552/aebb69 [doi] AB - Acceleration, velocity, and position can be combined in non-invasive movement decoding, but in this dataset only acceleration is measured; velocity and position are deterministic transforms obtained by integration. We therefore tested whether cross-order decoder fusion improves estimation in position space and whether any apparent gain remains after matching the spectral consequences of those transforms. Approach. We analysed single-trial MEG recordings from nine participants (18 runs; 2,160 trials) using a deterministic five-fold split. The primary outcome was within-trial Pearson correlation in position-residual space after removing trial-invariant trajectory. Acceleration-, velocity-, and position-based routes were fused and compared with same-order bagging, decorrelation-matched controls, and a bandwidth-matched pseudo-fusion of filtered copies of the direct position decoder. We additionally tested implementation sensitivity, residual-space R(2), non-positive-lag and causal pipelines, artefact sensitivity, multi-task architectures, and between-run transfer. Main results. Cross-order fusion exceeded same-order bagging by +0.0389 per-trial r (95% CI [+0.0288, +0.0499]; 9/9 participants). The bandwidth-matched pseudo-fusion exceeded bagging by +0.0424 and reproduced 109.0% of the offline margin, leaving E_cross - E_spec = -0.0035 (95% CI [-0.0105, +0.0032]; upper one-sided 95% bound +0.003). A nested-cross-validated smoothed single decoder likewise matched the ensemble. On residual-space R(2) with a training-fitted gain, the ordering reversed (E_bag -0.099, E_cross -0.120), showing that the correlation gain did not improve amplitude fidelity. With non-positive lags only, bandwidth matching explained 79% of the margin; under a fully causal zero-look-ahead pipeline, a smaller residual persisted and its mechanism was underpowered to resolve. Between-run transfer within the same visit retained only about 18% of within-run accuracy. Significance. In the primary offline regime, cross-order fusion gains can arise from integration-induced spectral smoothing without additional cross-order neural information. A bandwidth-matched surrogate should precede mechanistic interpretation when decoder routes are linked by deterministic transforms. In the causal regime, the advantage remains available online, but its mechanism remains unresolved. CI - (c) 2026 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved. FAU - Shin, Youmin AU - Shin Y AUID- ORCID: 0000-0003-3676-1090 AD - Seoul AI School, Seoul School of Integrated Sciences and Technologies, 504, 46 Ewhayeodae 2-gil, Seodaemun-gu, Seoul, Republic of Korea., Seodaemun-gu, Seoul, 03767, Korea (the Republic of). LA - eng PT - Journal Article DEP - 20261029 PL - England TA - J Neural Eng JT - Journal of neural engineering JID - 101217933 SB - IM OTO - NOTNLM OT - brain-computer interface OT - ensemble learning OT - magnetoencephalography OT - movement decoding OT - multi-task learning OT - single-trial reconstruction OT - spectral smoothing EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 18:53 PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 18:53 [entrez] AID - 10.1088/1741-2552/aebb69 [doi] PST - aheadofprint SO - J Neural Eng. 2026 Oct 29. doi: 10.1088/1741-2552/aebb69. PMID- 42956412 OWN - NLM STAT- Publisher LR - 20261029 IS - 1741-2552 (Electronic) IS - 1741-2552 (Linking) DP - 2026 Oct 29 TI - Source-aware forward modelling and detection limits of acoustoelectric neural recording. LID - 10.1088/1741-2552/aebb68 [doi] AB - Objective. Acoustoelectric recording uses focused ultrasound to select the region sampled by scalp or implanted electrodes. We relate its recorded voltage to source structure, terminal conditions and the carrier phase of the focus, and identify configurations that favour neural recording. Approach. We compare measured and simulated acoustoelectric fields in saline at seven incidence angles. We derive the first-order model from conservation of impressed and conduction current and relate it to conventional reciprocity. A finite-source benchmark examines how the travelling-wave focus weights distributed sources. Anatomical head-model simulations with six active cortical columns test source selection and detection limits. Main results. The interaction coefficient of saline is k(ae)= (0.95 +/- 0.11) x 10(-9)Pa(-1), consistent with theoretical predictions, and predicts a second phantom's response without refitting. For fixed impressed currents the complete first-order model recovers the conventional lead-field integral exactly. The carrier phase favours sources whose sensitivity kernel changes sign within half a wavelength along the beam. In the lead-field phantom the simulated peak response under current-clamped terminals, the analogue of a fixed impressed source, is an order of magnitude smaller than under voltage-clamped terminals. In the head model a focus selects one of six active columns, and the conventional lead-field integral reproduces the complete solution's scalp topography. At the FDA intensity reference an idealised model of contacts 2 to 5 mm from the cortex gives 3.4 to 53 nV at 500 kHz, whereas the scalp signal of the 1 MHz focus is 3.6 to 17 pV. Significance. Carrier frequency, beam orientation and contact position can be chosen to favour laminar cortical sources, including configurations that cancel substantially in conventional recordings. Scalp recording trades selectivity for sensitivity, whereas implanted contacts at 500 kHz are predicted to detect steady-state activity within 0.02 s to a few minutes, so focused ultrasound could add steerable sampling around them. CI - (c) 2026 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved. FAU - Chare, Christopher AU - Chare C AUID- ORCID: 0000-0003-1447-9625 AD - Interuniversity Micro-Electronic Centre, Kapeldreef 75, Leuven, 3001, Belgium. FAU - Haouari, Rachid AU - Haouari R AD - Interuniversity Micro-Electronic Centre, Kapeldreef 75, Leuven, 3001, Belgium. FAU - Volckaert, Klara AU - Volckaert K AD - Interuniversity Micro-Electronic Centre, Kapeldreef 75, Leuven, 3001, Belgium. FAU - Bottenberg, Agknaton AU - Bottenberg A AUID- ORCID: 0000-0003-2705-5549 AD - Interuniversity Micro-Electronic Centre, Kapeldreef 75, Leuven, 3001, Belgium. FAU - Haesler, Sebastian AU - Haesler S AD - Neuro-Electronics Research Flanders, Remisebosweg 1, Leuven, 3001, Belgium. FAU - Van Helleputte, Nick AU - Van Helleputte N AD - Interuniversity Micro-Electronic Centre, Kapeldreef 75, Leuven, 3001, Belgium. FAU - Rottenberg, Xavier AU - Rottenberg X AD - Interuniversity Micro-Electronic Centre, Kapeldreef 75, Leuven, 3001, Belgium. FAU - Houri, Samer AU - Houri S AD - Interuniversity Micro-Electronic Centre, Kapeldreef 75, Leuven, 3001, Belgium. FAU - Peumans, Peter AU - Peumans P AUID- ORCID: 0009-0006-8841-8357 AD - Interuniversity Micro-Electronic Centre, Kapeldreef 75, Leuven, 3001, Belgium. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - J Neural Eng JT - Journal of neural engineering JID - 101217933 SB - IM OTO - NOTNLM OT - acoustoelectric brain imaging OT - acoustoelectric effect OT - current source imaging OT - lead-field theory OT - ultrasound-based sensing EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 18:53 PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 18:53 [entrez] AID - 10.1088/1741-2552/aebb68 [doi] PST - aheadofprint SO - J Neural Eng. 2026 Oct 29. doi: 10.1088/1741-2552/aebb68. PMID- 42956406 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-4030 (Electronic) IS - 1350-4533 (Linking) DP - 2026 Oct 29 TI - Open-loop system identification of an AC motor-driven arterial flow generation platform: vessel-specific models and a parsimonious common ARX model. LID - 10.1088/1873-4030/aebb2f [doi] AB - Accurate reproduction of arterial flow waveforms is important for in vitro cardiovascular experiments and controlled biomedical flow platforms. This study characterizes the open-loop dynamics of an AC motor-inverter-centrifugal-pump platform using five experimentally recorded input-output datasets representing femoral, carotid, iliac, coronary, and aortic waveform conditions. The inverter command was the input, the magnetic flow-meter voltage was the output, and all original recordings were sampled at 100 Hz. Vessel-specific FIR, ARX, and ridge-regularized ARX models were screened separately. A common model was selected using nested chronological validation and assessed on untouched test segments using one-step prediction, recursive free-run simulation, and diagnostic analyses. Vessel-specific one-step validation fits ranged from 80.33% to 92.80%. The common ARX(5,4,80) model achieved mean one-step and free-run fits of 95.82% and 80.04%, respectively. All poles were inside the unit circle, but significant residual autocorrelation showed that the measured response was not fully explained. The common model provides a compact nominal representation of the tested platform. Its comparison with vessel-specific models is descriptive because it uses more training data, and cycle consistency within a recording does not establish between-session experimental reproducibility. CI - Creative Commons Attribution license. FAU - Serdar, Yilmaz AU - Serdar Y AUID- ORCID: 0000-0002-4316-3737 AD - Technical Science Vocational School, Istanbul University-Cerrahpasa, Buyukcekmece, Avcilar, Istanbul, 34500, Turkey. FAU - Yildiz, Huseyin AU - Yildiz H AUID- ORCID: 0000-0002-0575-3904 AD - Control and Automation Technology, Istanbul University-Cerrahpasa, Muhendislik Fakultesi A BLok Kat 6 Makine Muhendisligi Bolum, Avcilar, Istanbul, 34320, Turkey. FAU - Akay, Canan AU - Akay C AD - Istanbul University-Cerrahpasa, Vocational School of Technical Sciences, Department of Electronics and Automation, Avcilar, Istanbul, 34320, Turkey. FAU - Kaysi, Feyzi AU - Kaysi F AUID- ORCID: 0000-0001-6681-4574 AD - Department of Control and Automation, Istanbul University-Cerrahpasa, Vocational School Of Technical Sciences, Department Of Electronics And Automation, Hadimkoy, Istanbul, Istanbul, 34500, Turkey. FAU - Sedef, Herman AU - Sedef H AD - Yildiz Technical University Davutpasa Campus, Davutpasa Elektrik-Elektronik Muhendisligi, Istanbul, Istanbul, 34220, Turkey. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Med Eng Phys JT - Medical engineering & physics JID - 9422753 SB - IM OTO - NOTNLM OT - ARX model OT - arterial flow waveform OT - free-run validation OT - multi-experiment modelling OT - open-loop system identification OT - residual analysis OT - ridge regularization EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 18:53 PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 18:53 [entrez] AID - 10.1088/1873-4030/aebb2f [doi] PST - aheadofprint SO - Med Eng Phys. 2026 Oct 29. doi: 10.1088/1873-4030/aebb2f. PMID- 42956405 OWN - NLM STAT- Publisher LR - 20261029 IS - 2057-1976 (Electronic) IS - 2057-1976 (Linking) DP - 2026 Oct 29 TI - Evaluation of Dose to Medium and Dose to Water with the Acuros XB Algorithm for SABR: Clinical and Phantom-Based Observations. LID - 10.1088/2057-1976/aebb32 [doi] AB - Acuros XB (AXB) is a grid-based Boltzmann transport solver that can report dose either to medium (Dm) or to water (Dw). While Dm offers a more physically descriptive model of tissue interactions, Dw maintains continuity with established dosimetry protocols. This study investigated the impact of dose reporting mode on stereotactic ablative body radiotherapy (SABR) planning and verification. Approach: Fifteen previously treated SABR cases (five nodal, five bone, five lung) were retrospectively selected and recalculated using AXB in both Dm and Dw reporting modes. Dose-volume histogram (DVH) metrics for dose maximum and organs at risk were compared. Gamma analysis (2%/2 mm, global normalisation) was performed to assess spatial agreement. Independent verification was undertaken by using anthropomorphic phantoms with point dose ionisation chamber measurements. Main results: In soft tissue and lung, differences between Dw and Dm were negligible (< 2% in DVH metrics, within +/-4% of ionisation chamber phantom measurements), and all plans achieved high gamma pass rates (>/= 95% at 2%/2 mm). In bone larger discrepancies were observed, with DVH deviations up to 5.4% and reduced gamma pass rates. Dm differed in bone-equivalent material from phantom measurements by up to 7.7%, whereas Dw showed closer agreement with measurements. Significance: The choice of AXB reporting mode has tissue-dependent implications for SABR. While differences between Dm and Dw are generally small in soft tissue and lung, larger discrepancies may arise in bone and bone-equivalent materials, particularly in high dose gradient regions. Dw demonstrated closer agreement with anthropomorphic phantom verification measurements in bone-equivalent materials than Dm, which showed larger deviations from measured dose than typically accepted SABR verification tolerances. These findings highlight the importance of considering both tissue composition and verification methodology when interpreting dose calculations using AXB. CI - (c) 2026 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved. FAU - Tassell, Nicholas Michael AU - Tassell NM AUID- ORCID: 0009-0006-6757-7834 AD - Radiotherapy Physics, Maidstone and Tunbridge Wells NHS Trust, Maidstone General Hospital, Maidstone, ME16 9QQ, United Kingdom of Great Britain and Northern Ireland. FAU - Vashistha, Apoorv AU - Vashistha A AUID- ORCID: 0009-0001-9817-7781 AD - Radotherapy Physics, Maidstone and Tunbridge Wells NHS Trust, Maidstone General Hospital, Maidstone, ME16 9QQ, United Kingdom of Great Britain and Northern Ireland. FAU - Mendoza Torres-Quevedo, Paz AU - Mendoza Torres-Quevedo P AUID- ORCID: 0009-0003-5780-1167 AD - Radiotherapy Physics, Maidstone and Tunbridge Wells NHS Trust, Maidstone General Hospital, Maidstone, ME16 9QQ, United Kingdom of Great Britain and Northern Ireland. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Biomed Phys Eng Express JT - Biomedical physics & engineering express JID - 101675002 SB - IM OTO - NOTNLM OT - Acuros XB OT - Dose to medium (Dm) OT - Dose to water (Dw) OT - Stereotactic ablative body radiotherapy (SABR) EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 18:53 PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 18:53 [entrez] AID - 10.1088/2057-1976/aebb32 [doi] PST - aheadofprint SO - Biomed Phys Eng Express. 2026 Oct 29. doi: 10.1088/2057-1976/aebb32. PMID- 42956404 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-4030 (Electronic) IS - 1350-4533 (Linking) DP - 2026 Oct 29 TI - Optimisation of Metal Augments in Revision Total Hip Arthroplasty: Current Advances and Future Directions. LID - 10.1088/1873-4030/aebb2e [doi] AB - Revision total hip arthroplasty (THA) is a challenging procedure, especially when addressing acetabular bone defects that compromise implant stability and long-term fixation. Metal augmentation is a common solution to restore structural integrity and ease implant placement. Recent literature has increasingly addressed clinical outcomes, porous implant technologies, and patient-specific reconstruction; however, an integrated perspective linking defect morphology, implant design, biomechanics, computational optimisation, manufacturing, and clinical translation remains limited. This narrative review critically discusses current metal augmentation technologies in terms of biomechanical performance, defect characteristics, and clinical outcomes. The main limitations, such as poor geometrical conformity, stress shielding, micromotion, and fixation failure, are discussed along with the role of computational modelling in evaluating implant behaviour. Critically, the review highlights the lack of integrated frameworks linking defect morphology, biomechanical performance, and clinical outcomes. Emerging technologies like additive manufacturing, patient-specific implants, and digital modelling are discussed from a translational perspective. It also emphasises the need for clinically validated, biomechanics-driven evaluation strategies to guide acetabular reconstruction and improve patient outcomes in revision THA. CI - (c) 2026 Institute of Physics and Engineering in Medicine. All rights, including for text and data mining, AI training, and similar technologies, are reserved. FAU - Singh, Gurpreet AU - Singh G AUID- ORCID: 0000-0001-5910-3213 AD - School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, England, E1 4NS, United Kingdom of Great Britain and Northern Ireland. FAU - Alonso Rasgado, M T AU - Alonso Rasgado MT AUID- ORCID: 0000-0002-7594-145X AD - Queen Mary University of London, School of Engineering and Materials Science, Queen Mary University of London, United Kingdom., London, E1 4NS, United Kingdom of Great Britain and Northern Ireland. FAU - Miguel-Andres, Israel AU - Miguel-Andres I AUID- ORCID: 0000-0002-9433-7864 AD - CIATEC, Leon, 37545, Guanajuato, Mexico, Leon, Gto., 37545, Mexico. FAU - Bailey, Colin G AU - Bailey CG AD - School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, --- Select One ---, E1 4NS, United Kingdom of Great Britain and Northern Ireland. FAU - Board, Tim N AU - Board TN AD - Wrightington Wigan and Leigh NHS Foundation Trust, Wigan and Leigh NHS Foundation Trust, Lancashire, United Kingdom., Wigan, England, WN1 2NN, United Kingdom of Great Britain and Northern Ireland. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Med Eng Phys JT - Medical engineering & physics JID - 9422753 SB - IM OTO - NOTNLM OT - Revision total hip arthroplasty OT - acetabular reconstruction OT - biomechanical optimisation OT - finite element analysis OT - metal augments OT - patient-specific implants EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 18:53 PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 18:53 [entrez] AID - 10.1088/1873-4030/aebb2e [doi] PST - aheadofprint SO - Med Eng Phys. 2026 Oct 29. doi: 10.1088/1873-4030/aebb2e. PMID- 42956400 OWN - NLM STAT- Publisher LR - 20261029 IS - 1476-1645 (Electronic) IS - 0002-9637 (Linking) DP - 2026 Oct 29 TI - Accuracy of Rapid Tests for the Diagnosis of Acute Dengue in Southern Nigeria. LID - tpmd260359 [pii] LID - 10.4269/ajtmh.26-0359 [doi] AB - Dengue is an emerging public health concern in West Africa. Although rapid diagnostic tests (RDTs) have facilitated dengue diagnosis, surveillance, and outbreak detection in other endemic regions, there is a paucity of data on their accuracy in African populations. Our objective was to evaluate the diagnostic accuracy of commercial dengue RDTs in southern Nigeria. A retrospective cross-sectional study was conducted in Benin City, Nigeria, using archived plasma samples previously tested with reverse transcriptase-polymerase chain reaction (RT-PCR) as the reference method. Laboratory personnel blinded to the reference test results evaluated a dengue nonstructural (NS) 1 antigen RDT and a combined IgM/IgG antibody test (assessing the individual antibody components separately). Sensitivity, specificity, and positive and negative predictive values (PPV, NPV) were estimated for NS1 antigen, IgM, and IgG and reported with 95% confidence intervals (CIs). Of 145 samples selected for the evaluation, 95 (65.5%) were dengue RT-PCR-positive. The sensitivity, specificity, PPV, and NPV of the NS1 antigen RDT with 95% CI were 94.7% (91.5%-98.5%), 100% (98.7%-100.0%), 100% (97.9%-100.0%), and 90.9% (83.5%-98.5%), respectively; its overall accuracy was 96.6% in comparison with 53.8% for IgG and 31.7% for IgM. The area under the receiver operating characteristic curve was 0.974 for NS1 antigen, 0.5 for IgG, and 0.427 for IgM. Although both IgG and IgM components of the antibody RDT evaluated demonstrated poor diagnostic metrics for acute dengue detection, the NS1 antigen RDT had excellent diagnostic parameters, showing promise as a faster alternative for primary diagnosis and routine surveillance of dengue in Southern Nigeria. FAU - Osaigbovo, Iriagbonse I AU - Osaigbovo II AD - Department of Medical Microbiology, College of Medical Sciences, University of Benin, Benin City, Nigeria. AD - Department of Medical Microbiology, University of Benin Teaching Hospital, Benin City, Nigeria. FAU - Igbarumah, Isaac O AU - Igbarumah IO AD - Molecular Diagnostic and Virology Laboratory, University of Benin Teaching Hospital, Benin City, Nigeria. FAU - Bazuaye, Alex AU - Bazuaye A AD - Molecular Diagnostic and Virology Laboratory, University of Benin Teaching Hospital, Benin City, Nigeria. FAU - Igbokwe, Chizoba AU - Igbokwe C AD - Molecular Diagnostic and Virology Laboratory, University of Benin Teaching Hospital, Benin City, Nigeria. FAU - Okwara, Benson U AU - Okwara BU AD - Department of Medicine, University of Benin Teaching Hospital, Benin City, Nigeria. FAU - Osaigbovo, Emmanuel AU - Osaigbovo E AD - Department of Surgery, University of Benin Teaching Hospital, Benin City, Nigeria. FAU - Yahaya, Mohammed AU - Yahaya M AD - Department of Medical Microbiology and Parasitology, Usman Danfodio University Teaching Hospital, Sokoto, Nigeria. FAU - Olufemi, Abayomi A AU - Olufemi AA AD - Ministry of Defence, Abuja, Nigeria. FAU - Ogboghodo, Esohe O AU - Ogboghodo EO AD - Department of Public Health and Community Medicine, University of Benin Teaching Hospital, Benin City, Nigeria. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Am J Trop Med Hyg JT - The American journal of tropical medicine and hygiene JID - 0370507 SB - IM EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 18:33 PHST- 2026/05/28 00:00 [received] PHST- 2026/08/25 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 18:33 [entrez] AID - tpmd260359 [pii] AID - 10.4269/ajtmh.26-0359 [doi] PST - aheadofprint SO - Am J Trop Med Hyg. 2026 Oct 29:tpmd260359. doi: 10.4269/ajtmh.26-0359. PMID- 42956398 OWN - NLM STAT- Publisher LR - 20261029 IS - 1476-1645 (Electronic) IS - 0002-9637 (Linking) DP - 2026 Oct 29 TI - Predictive Surveillance for Andes Virus Transmission in the Americas. LID - tpmd260470 [pii] LID - 10.4269/ajtmh.26-0470 [doi] AB - The 2026 Andes virus outbreak linked to the M/V Hondius cruise ship exposed limitations in conventional, case-based surveillance for zoonotic threats with potential person-to-person transmission. In the present opinion and analysis article, the authors argue that predictive surveillance, supported by artificial intelligence, ecological modeling, and the One Health approach, can strengthen preparedness for hantavirus transmission in the Americas. Current evidence suggests that selected models that incorporate epidemiological, climatic, environmental, and rodent-related variables may provide an anticipatory window of 2 to 12 months, depending on context and data inputs. However, implementation requires more than technical capacity. It depends on interoperable multisectoral data, transparent model governance, local validation, sustainable financing, and technology transfer to avoid widening inequities between health systems. Predictive surveillance should complement conventional surveillance by supporting risk communication, rodent monitoring, laboratory preparedness, and targeted public health action in areas where ecological and epidemiological signals converge. FAU - Visconti-Lopez, Fabriccio J AU - Visconti-Lopez FJ AD - Escuela de Posgrado, Universidad Continental, Lima, Peru. FAU - Diaz Vallejo, Jhony Alejandro AU - Diaz Vallejo JA AD - Department of Basic Health Sciences, Research Group in Nutrition, Metabolism and Food Security, University of Caldas, Manizales, Colombia. FAU - Lozada Martinez, Ivan David AU - Lozada Martinez ID AD - Center for Meta-Research and Scientometrics in Biomedical Sciences, Barranquilla, Colombia. AD - Biomedical Scientometrics and Evidence-Based Research, Department of Health Sciences, Universidad de la Costa, Barranquilla, Colombia. AD - Clinica Iberoamerica, Clinica Colsanitas S.A, Barranquilla, Colombia. FAU - Cuji Galarza, Wendy Dayanna AU - Cuji Galarza WD AD - Azuay Provincial Health Directorate, Ecuador Ministry of Health, Chordeleg, Ecuador. FAU - Alvarez Lopez, Maria Elena AU - Alvarez Lopez ME AD - Grupo de Investigacion en Genetica, Bioidiversidad y Manejo de Ecosistemas, University of Caldas, Manizales, Colombia. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Am J Trop Med Hyg JT - The American journal of tropical medicine and hygiene JID - 0370507 SB - IM EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 18:32 PHST- 2026/07/17 00:00 [received] PHST- 2026/09/03 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 18:32 [entrez] AID - tpmd260470 [pii] AID - 10.4269/ajtmh.26-0470 [doi] PST - aheadofprint SO - Am J Trop Med Hyg. 2026 Oct 29:tpmd260470. doi: 10.4269/ajtmh.26-0470. PMID- 42956389 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1526-632X (Electronic) IS - 0028-3878 (Linking) VI - 107 IP - 10 DP - 2026 Nov 24 TI - Exclusion of Arteriovenous Shunts in Intracerebral Hemorrhage Using MRI Arterial Spin Labeling: A Prospective Multicenter Study. PG - e218705 LID - 10.1212/WNL.0000000000218705 [doi] AB - BACKGROUND AND OBJECTIVES: Identifying intracranial arteriovenous shunts (AVSs), chiefly arteriovenous malformations (AVMs) and dural arteriovenous fistulas (dAVFs), after nontraumatic intracerebral hemorrhage (ICH) is critical to guide management and prevent recurrence. AVSs are the leading secondary causes of ICH but typically require digital subtraction angiography (DSA) for diagnosis, an invasive, ionizing radiation-based procedure that carries a non-negligible risk of complications and is not readily available in many centers. We assessed the diagnostic accuracy of arterial spin labeling (ASL), a noninvasive MRI technique for detecting AVSs during the etiologic workup of acute ICH. METHODS: We conducted a prospective multicenter diagnostic accuracy study, across 3 French tertiary hospitals within the ongoing OPTICH cohort (October 2022-October 2025). Consecutive adults with nontraumatic ICH who underwent standardized MRI including ASL within the first week were included. ASL perfusion maps were independently evaluated by 2 blinded neuroradiologists and rated positive when focal hyperperfusion was observed adjacent to the hematoma. Final ICH etiology was adjudicated using all available imaging and follow-up data, with DSA confirmation for all AVSs. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and overall accuracy were calculated, using the final etiologic diagnosis as the reference standard. RESULTS: Among 537 enrolled patients, 277 met inclusion criteria (median age 71 years [interquartile range 60-80]; 163 (58.8%) men). An AVS was confirmed in 24 patients (8.7%; 23 AVMs and 1 dAVFs). Interobserver agreement for ASL rating was excellent (kappa = 0.88). ASL demonstrated a sensitivity of 95.8% (95% CI 79.8-99.3), specificity of 94.5% (90.9-96.7), PPV of 62.2% (46.1-75.9), and NPV of 99.6% (98.1-99.9), with an overall accuracy of 94.6% (91.3-96.7). All AVSs identified on initial DSA were detected by ASL; 3 ASL-positive AVSs were occult on initial DSA. DISCUSSION: In an unselected, low-prevalence adult ICH population, ASL demonstrated excellent diagnostic accuracy and a near-perfect NPV, indicating that a negative ASL reliably excludes AVS. Main limitations include selective DSA use, few non-AVM shunts, and modest scanner heterogeneity. Pending external validation, these findings support systematic incorporation of ASL as a gatekeeper to DSA into standard MRI protocols during ICH etiologic workup. FAU - Beaufort, Quentin AU - Beaufort Q AUID- ORCID: 0000-0001-6618-1842 AD - Diagnostic and Interventional Neuroradiology, Tours University Hospital, France. AD - Centre d'Investigation Clinique - Innovation Technologique (CIC-IT) 1415, Tours University Hospital Tours, France. AD - Universite de Tours, INSERM, Imaging Brain & Neuropsychiatry iBraiN U1253, 37032, Tours, France. FAU - Agripnidis, Thibault AU - Agripnidis T AUID- ORCID: 0000-0003-2772-2954 AD - Diagnostic and Interventional Neuroradiology, Tours University Hospital, France. AD - Department of Neuroradiology, APHM La Timone, Marseille, France. FAU - Amar, Valentin AU - Amar V AUID- ORCID: 0009-0003-6847-1634 AD - Centre d'Investigation Clinique - Innovation Technologique (CIC-IT) 1415, Tours University Hospital Tours, France. AD - Universite de Tours, INSERM, Imaging Brain & Neuropsychiatry iBraiN U1253, 37032, Tours, France. AD - Neurology department, Tours University Hospital, France. FAU - Pandaure, Benjamin AU - Pandaure B AD - Neurology department, Tours University Hospital, France. FAU - Bretonniere, Arnaud AU - Bretonniere A AUID- ORCID: 0009-0004-6685-3337 AD - Neurology department, Tours University Hospital, France. FAU - Cottier, Jean-Philippe AU - Cottier JP AUID- ORCID: 0000-0001-9166-212X AD - Department of Diagnostic and Interventionnal Neuroradiology, Tours University Hospital, France. FAU - L'Allinec, Vincent AU - L'Allinec V AD - Department of Radiology, University Hospital of Angers, France. FAU - Godard, Sophie AU - Godard S AUID- ORCID: 0009-0006-7453-7080 AD - Neurology department, Angers University Hospital, France; and. FAU - Codron, Philippe AU - Codron P AUID- ORCID: 0000-0003-1745-8299 AD - Neurology department, Angers University Hospital, France; and. FAU - Ifergan, Heloise AU - Ifergan H AUID- ORCID: 0000-0001-6582-6581 AD - Diagnostic and Interventional Neuroradiology, Tours University Hospital, France. FAU - Cohen, Clara AU - Cohen C AUID- ORCID: 0000-0002-8200-2784 AD - University Hospital Center of Orleans, Neuroradiology Department, France. FAU - Pasi, Marco AU - Pasi M AUID- ORCID: 0000-0001-9976-2459 AD - Centre d'Investigation Clinique - Innovation Technologique (CIC-IT) 1415, Tours University Hospital Tours, France. AD - Universite de Tours, INSERM, Imaging Brain & Neuropsychiatry iBraiN U1253, 37032, Tours, France. AD - Neurology department, Tours University Hospital, France. FAU - Boulouis, Gregoire AU - Boulouis G AUID- ORCID: 0000-0001-8422-9205 AD - Diagnostic and Interventional Neuroradiology, Tours University Hospital, France. AD - Centre d'Investigation Clinique - Innovation Technologique (CIC-IT) 1415, Tours University Hospital Tours, France. AD - Universite de Tours, INSERM, Imaging Brain & Neuropsychiatry iBraiN U1253, 37032, Tours, France. CN - OPTICH collaborators LA - eng PT - Journal Article PT - Multicenter Study DEP - 20261029 PL - United States TA - Neurology JT - Neurology JID - 0401060 RN - 0 (Spin Labels) SB - IM MH - Humans MH - Prospective Studies MH - *Cerebral Hemorrhage/diagnostic imaging/etiology MH - Spin Labels MH - Female MH - Male MH - Aged MH - Middle Aged MH - *Magnetic Resonance Imaging/methods MH - Angiography, Digital Subtraction MH - *Intracranial Arteriovenous Malformations/diagnostic imaging/complications MH - *Arteriovenous Fistula/diagnostic imaging/complications MH - Sensitivity and Specificity MH - Perfusion Magnetic Resonance Imaging EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:29 CRDT- 2026/10/29 17:52 PHST- 2026/10/29 23:29 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 17:52 [entrez] AID - 10.1212/WNL.0000000000218705 [doi] PST - ppublish SO - Neurology. 2026 Nov 24;107(10):e218705. doi: 10.1212/WNL.0000000000218705. Epub 2026 Oct 29. PMID- 42956385 OWN - NLM STAT- Publisher LR - 20261029 IS - 1537-4505 (Electronic) IS - 1531-7129 (Linking) DP - 2026 Oct 27 TI - First Experience With a New Active Bone Conduction Device in Pediatric Patients. LID - 10.1097/MAO.0000000000005095 [doi] AB - OBJECTIVE: The aim of this retrospective analysis was to evaluate the feasibility, surgical safety, and early audiological benefit of the new active transcutaneous bone conduction implant Sentio in pediatric patients. DESIGN: Retrospective case series. STUDY SAMPLE: Seven children (8 ears), aged 3.6 to 9.7 years, underwent implantation of a Sentio system between December 2024 and March 2025 for congenital aural atresia (6 ears) or single-sided deafness due to cochlear nerve aplasia (2 ears). RESULTS: All surgeries were completed successfully using the Sentio Ti Implant. Limited exposure of the dura, sigmoid sinus, and/or mastoid air cells occurred during bone-bed preparation, but there was no laceration, bleeding, cerebrospinal fluid leak, compression, or clinical sequela. No perioperative or postoperative adverse events occurred through initial fitting. Bone conduction thresholds remained stable postoperatively, confirming preservation of cochlear reserve. Mean Sentio-aided free-field thresholds were 22.5, 20.0, 18.6, and 18.6 dB HL at 0.5, 1, 2, and 4 kHz, respectively. Word recognition scores at initial fitting averaged 96.3% in all patients (range: 85-100%) at a conversational level of 65 dB SPL. The relatively flat transducer and flexible band fixation facilitated safe implantation in young children with thin cranial bone. CONCLUSION: Sentio Ti implantation was technically feasible in this selected cohort, with no clinical adverse events through initial fitting and favorable early aided outcomes. Larger prospective studies with standardized testing and longer follow-up are required to assess long-term safety, skull growth, implant stability, MRI-related limitations, and sustained benefit. CI - Copyright (c) 2026, Otology & Neurotology, Inc. FAU - Prenzler, Nils AU - Prenzler N AD - Department of Otorhinolaryngology and Cluster of Excellence "Hearing4all.connects", Hannover Medical School, Hannover, Germany. FAU - Higgins, Marc AU - Higgins M AUID- ORCID: 0009-0009-9726-9359 FAU - Lenarz, Thomas AU - Lenarz T FAU - Maier, Hannes AU - Maier H FAU - Ghoncheh, Mohammad AU - Ghoncheh M FAU - Busch, Susan AU - Busch S LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Otol Neurotol JT - Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology JID - 100961504 SB - IM OTO - NOTNLM OT - Sentio system OT - bone conduction devices OT - bone thickness OT - pediatric patients COIS- The authors disclose no conflicts of interest. EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 17:52 PHST- 2026/01/06 00:00 [received] PHST- 2026/09/05 00:00 [accepted] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 17:52 [entrez] AID - 00129492-990000000-01353 [pii] AID - 10.1097/MAO.0000000000005095 [doi] PST - aheadofprint SO - Otol Neurotol. 2026 Oct 27. doi: 10.1097/MAO.0000000000005095. PMID- 42956384 OWN - NLM STAT- Publisher LR - 20261029 IS - 1480-3275 (Electronic) IS - 0008-4166 (Linking) DP - 2026 Oct 29 TI - Comparative genomic analysis for selected members of the Ophiostomatales: insights into biosynthetic gene clusters and CAZymes. LID - 10.1139/cjm-2026-0110 [doi] AB - The Order Ophiostomatales includes ecologically diverse fungi, and many are associates of bark and/or ambrosia beetles. This order includes species that cause tree diseases, and many members are sapwood staining fungi. Despite their ecological and economic importance, the genomic basis of their adaptations remains poorly understood. Here, we present annotated genome assemblies for six species (seven strains) spanning three genera within the Ophiostomatales. Genomes ranged from 21 to 32 Mb with high completeness scores. CAZyme analyses revealed broadly conserved repertoires dominated by glycoside hydrolases and glycosyltransferases, with substantial representation of lignin-and cellulose-associated enzymes and marked quantitative variation among taxa, including expanded oxidative auxiliary activity families in Ophiostoma quercus and Leptographium truncatum and a reduced profile in Ceratocystiopsis minima. antiSMASH predicted 12-19 biosynthetic gene clusters (BGCs) among the seven genomes. Rather than only broad metabolite classes, comparative analysis revealed conservation of specific secondary metabolite families, including a putative 1,8-dihydroxynaphthalene (DHN) melanin cluster present in all genomes, a conserved fujikurin-like type I polyketide synthase (T1PKS) cluster present in all genomes except L. truncatum, and an Ophiostoma-specific NRPSbased metallophore/siderophore-like cluster potentially involved in iron acquisition. The genomic data provide new insights into the metabolic capacity and potential ecological differentiation of the Ophiostomatales. FAU - Abed, Sawsan AU - Abed S AD - University of Manitoba, Department of Microbiology, Winnipeg, Manitoba, Canada; abeds@myumanitoba.ca. FAU - Kumar, Ayush AU - Kumar A AD - University of Manitoba, Microbiology, Winnipeg, Manitoba, Canada; Ayush.Kumar@umanitoba.ca. FAU - Hausner, Georg AU - Hausner G AD - University of Manitoba, Department of Microbiology, Winnipeg, Canada; Georg.Hausner@umanitoba.ca. LA - eng PT - Journal Article DEP - 20261029 PL - Canada TA - Can J Microbiol JT - Canadian journal of microbiology JID - 0372707 SB - IM EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 17:42 PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 17:42 [entrez] AID - 10.1139/cjm-2026-0110 [doi] PST - aheadofprint SO - Can J Microbiol. 2026 Oct 29. doi: 10.1139/cjm-2026-0110. PMID- 42956382 OWN - NLM STAT- Publisher LR - 20261029 IS - 1558-9110 (Electronic) IS - 1058-0360 (Linking) DP - 2026 Oct 29 TI - Disparate Practice Patterns in Dysphagia Diagnostics With Flexible Endoscopic Evaluation of Swallowing: An Exploratory Study. PG - 1-9 LID - 10.1044/2026_AJSLP-26-00048 [doi] AB - PURPOSE: The purpose of this study was, first, to explore current intraprofessional flexible endoscopic evaluation of swallowing (FEES) interpretation practices, expecting lack of methodological consensus. Second, it aimed to compare penetration-aspiration scale (PAS) ratings on FEES exams using Langmore-defined versus Visual Analysis of Swallowing Efficiency and Safety (VASES)-defined anatomical boundaries, hypothesizing that using the Langmore framework would yield more "abnormal" scores than the VASES, due to more conservatively defined anatomical boundaries. METHOD: FEES training course vendors and FEES clinical practitioners across the United States were surveyed regarding the anatomical boundaries they employ. Expert raters at a tertiary care center reviewed recorded FEES exams and rated PAS scores using either Langmore or VASES boundaries. Statistical analyses compared (a) the frequency of normal versus abnormal scores and (b) the frequency of PAS 1 and PAS 3 between the frameworks, representing the threshold for dysphagia diagnosis. RESULTS: There is notable intraprofessional inconsistency regarding FEES exam interpretation. There was a significant difference in the rates of normal versus abnormal PAS scores, with Langmore boundaries yielding more abnormal scores than VASES. Most notably, a significant difference was found in the frequency of PAS 1 (no airway invasion) versus PAS 3 (sustained penetration) between frameworks. CONCLUSIONS: Two framework options guide PAS ratings, which differ in their defined anatomical boundaries, directly impacting dysphagia diagnosis. Experienced clinicians adhering to the highest standards of evidence-based practice may still regularly misdiagnose dysphagia with wide-ranging implications for patients. The findings highlight a need to establish a consistent approach to dysphagia diagnostics with FEES and offer a concrete area of opportunity for improved standardization and accuracy. SUPPLEMENTAL MATERIAL: https://doi.org/10.23641/asha.34790430. FAU - Ashinoff, Samantha AU - Ashinoff S AUID- ORCID: 0009-0006-8854-7909 AD - Division of Speech-Language Pathology, Department of Otolaryngology, Boston Medical Center, MA. FAU - Karthikeyan, Gayathri AU - Karthikeyan G AUID- ORCID: 0009-0005-5248-6107 AD - Department of Biostatistics, Boston University School of Public Health, MA. FAU - Gottlieb, Amy AU - Gottlieb A AUID- ORCID: 0009-0004-1090-5281 AD - Division of Speech-Language Pathology, Department of Otolaryngology, Boston Medical Center, MA. AD - Department of Otolaryngology-Head & Neck Surgery, Boston University Chobanian & Avedisian School of Medicine, MA. FAU - McNally, Edel AU - McNally E AUID- ORCID: 0000-0003-0957-0993 AD - Division of Speech-Language Pathology, Department of Otolaryngology, Boston Medical Center, MA. FAU - O'Gorman, Emily AU - O'Gorman E AUID- ORCID: 0009-0009-0505-0684 AD - Division of Speech-Language Pathology, Department of Otolaryngology, Boston Medical Center, MA. FAU - Pisegna, Jessica M AU - Pisegna JM AUID- ORCID: 0000-0003-0794-8964 AD - Division of Speech-Language Pathology, Department of Otolaryngology, Boston Medical Center, MA. AD - Department of Otolaryngology-Head & Neck Surgery, Boston University Chobanian & Avedisian School of Medicine, MA. AD - Department of Speech, Language & Hearing Sciences, Sargent College of Health & Rehabilitation Sciences, Boston University, MA. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Am J Speech Lang Pathol JT - American journal of speech-language pathology JID - 9114726 SB - IM EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 17:12 PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 17:12 [entrez] AID - 10.1044/2026_AJSLP-26-00048 [doi] PST - aheadofprint SO - Am J Speech Lang Pathol. 2026 Oct 29:1-9. doi: 10.1044/2026_AJSLP-26-00048. PMID- 42956376 OWN - NLM STAT- Publisher LR - 20261029 IS - 1944-8252 (Electronic) IS - 1944-8244 (Linking) DP - 2026 Oct 30 TI - Mie Scattering-Enhanced Fluorinated Polyether Ether Ketone Nanofibrous Films for Efficient Radiative Heat Dissipation. LID - 10.1021/acsami.6c17640 [doi] AB - This study develops a high-performance radiative cooling (RC) composite film based on electrospinning fluorinated polyether ether ketone, incorporating titanium dioxide (TiO2) and hexagonal boron nitride (h-BN) as functional fillers to achieve exceptional spectral selectivity with a solar reflectance of 96.51% and mid-infrared emissivity of 95.12%. The three-dimensional porous fibrous network endows the film with outstanding RC performance, achieving a maximum sub-ambient temperature reduction of 14.63 degrees C under direct sunlight and a temperature reduction of 8.66 degrees C relative to the bare vehicle model in small-scale outdoor vehicle tests, thereby validating the significant cooling advantage of the electrospinning film under real-world service conditions. The synergistic effect of the porous fibrous network and intrinsically low-surface-energy matrix imparts self-cleaning functionality to the film (water contact angle of 151.46 degrees and sliding angle of approximately 5 degrees ), while solar reflectance degradation remains below 3% after the aging test, demonstrating long-term outdoor service reliability. EnergyPlus building energy simulations confirmed that the film achieved annual energy savings exceeding 6% in tropical arid cities, with positive net energy savings across the vast majority of global climate regions. This film provides a comprehensive design paradigm for high-performance multifunctional RC materials, with broad application prospects for low-carbon building energy conservation and outdoor thermal management. CI - (c) 2026 American Chemical Society. FAU - Gu, Yuxuan AU - Gu Y AUID- ORCID: 0009-0002-5576-3649 AD - Key Laboratory of High Performance Plastics, Ministry of Education, National & Local Joint Engineering Laboratory for Synthesis Technology of High Performance Polymer, College of Chemistry, Jilin University, Changchun130012, P. R. China. FAU - Chen, Rui AU - Chen R AD - School of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou510006, P. R. China. FAU - Zhou, Changze AU - Zhou C AD - Key Laboratory of High Performance Plastics, Ministry of Education, National & Local Joint Engineering Laboratory for Synthesis Technology of High Performance Polymer, College of Chemistry, Jilin University, Changchun130012, P. R. China. FAU - Li, Lei AU - Li L AD - Key Laboratory of High Performance Plastics, Ministry of Education, National & Local Joint Engineering Laboratory for Synthesis Technology of High Performance Polymer, College of Chemistry, Jilin University, Changchun130012, P. R. China. FAU - Bai, Yageng AU - Bai Y AD - College of Chemical and Pharmaceutical Engineering, Jilin University of Chemical Technology, Jilin132022, P. R. China. FAU - Mu, Jianxin AU - Mu J AUID- ORCID: 0000-0002-3223-6419 AD - Key Laboratory of High Performance Plastics, Ministry of Education, National & Local Joint Engineering Laboratory for Synthesis Technology of High Performance Polymer, College of Chemistry, Jilin University, Changchun130012, P. R. China. LA - eng PT - Journal Article DEP - 20261030 PL - United States TA - ACS Appl Mater Interfaces JT - ACS applied materials & interfaces JID - 101504991 SB - IM OTO - NOTNLM OT - electrostatic spinning OT - energy saving OT - polyether ether ketone OT - radiative cooling OT - self-cleaning EDAT- 2026/10/29 23:28 MHDA- 2026/10/29 23:28 CRDT- 2026/10/29 17:02 PHST- 2026/08/25 00:00 [received] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/29 23:28 [medline] PHST- 2026/10/29 23:28 [pubmed] PHST- 2026/10/29 17:02 [entrez] AID - 5461242 [pii] AID - 10.1021/acsami.6c17640 [doi] PST - aheadofprint SO - ACS Appl Mater Interfaces. 2026 Oct 30. doi: 10.1021/acsami.6c17640. PMID- 42956361 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1860-7187 (Electronic) IS - 1860-7179 (Linking) VI - 21 IP - 21 DP - 2026 Nov 12 TI - 7-(Chloroquinolin-4-yl) Sulfanyl Derivatives, Optimization of the Synthesis, In Silico ADMET Profiling Studies, and Evaluation of the Biological Activity Against Leishmania mexicana. LID - e70550 [pii] LID - 10.1002/cmdc.70550 [doi] AB - Leishmaniasis is a parasitic human infection that constitutes a major global health challenge due to limited therapeutic options, toxicity, resistance, and high costs of the drugs approved as therapy. In this study, a group of 7-chloroquinoline derivatives (5-12) was synthesized via an optimized route and evaluated against the human parasite Leishmania mexicana. Compounds were fully characterized and showed favorable in silico ADME/Tox profiles, including high predicted gastrointestinal absorption (>93%), although limited solubility and potential hepatotoxicity were observed for several derivatives. Biological assays demonstrated significant antileishmanial activity. Interestingly, intracellular amastigote assays revealed nanomolar potency and very high selectivity index (SI), with compound 12 exhibiting IC(50) = 2.5 nM (SI = 21.030) and compound 9 IC(50) = 5.2 nM (SI = 7.384). Mechanistic studies demonstrated concentration-dependent mitochondrial membrane depolarization and acidocalcisome alkalinization. Since these two organelles are both involved in bioenergetics and in parasite intracellular Ca(2+) homeostasis, the results support the notion that intracellular Ca(2+) disruption is an essential target for different drug actions. Overall, these findings support a multitarget mechanism and position these quinoline derivatives as promising chemical scaffolds for the development of improved antileishmanial strategies. CI - (c) 2026 Wiley-VCH GmbH. FAU - Gelvez, Angelo AU - Gelvez A AUID- ORCID: 0009-0009-4852-8625 AD - Unidad de Senalizacion Celular y Bioquimica de Parasitos, Instituto de Estudios Avanzados (IDEA), Caracas, Venezuela. AD - Facultad de Farmacia, Universidad Central de Venezuela, Caracas, Venezuela. FAU - Labrador-Fagundez, Liesangerli AU - Labrador-Fagundez L AD - Unidad de Senalizacion Celular y Bioquimica de Parasitos, Instituto de Estudios Avanzados (IDEA), Caracas, Venezuela. FAU - Stella, Bruno G AU - Stella BG AD - Unidad de Senalizacion Celular y Bioquimica de Parasitos, Instituto de Estudios Avanzados (IDEA), Caracas, Venezuela. FAU - Rivero, Sugeins AU - Rivero S AD - Unidad de Senalizacion Celular y Bioquimica de Parasitos, Instituto de Estudios Avanzados (IDEA), Caracas, Venezuela. FAU - Ramirez, Yaniska AU - Ramirez Y AD - Unidad de Senalizacion Celular y Bioquimica de Parasitos, Instituto de Estudios Avanzados (IDEA), Caracas, Venezuela. FAU - Vidal, Thalia AU - Vidal T AD - Unidad de Senalizacion Celular y Bioquimica de Parasitos, Instituto de Estudios Avanzados (IDEA), Caracas, Venezuela. FAU - Martinez-Sotillo, Nathalia AU - Martinez-Sotillo N AD - Unidad de Senalizacion Celular y Bioquimica de Parasitos, Instituto de Estudios Avanzados (IDEA), Caracas, Venezuela. FAU - Suarez, Alirica AU - Suarez A AUID- ORCID: 0000-0002-3317-5179 AD - Unidad de Productos Naturales, Facultad de Farmacia, Universidad Central de Venezuela, Caracas, Venezuela. FAU - Chavez, Katiuska AU - Chavez K AUID- ORCID: 0000-0003-4103-9799 AD - Unidad de Productos Naturales, Facultad de Farmacia, Universidad Central de Venezuela, Caracas, Venezuela. FAU - Ramirez, Hegira AU - Ramirez H AUID- ORCID: 0000-0003-1916-6118 AD - Universidad ECOTEC, Samborondon, Guayaquil, Guayas, Ecuador. FAU - Blanco, Zuleyma AU - Blanco Z AD - Laboratorio de Sintesis Organica, Facultad de Farmacia, Universidad Central de Venezuela, Caracas, Venezuela. FAU - Gutierrez, Joyce AU - Gutierrez J AD - Laboratorio de Sintesis Organica, Facultad de Farmacia, Universidad Central de Venezuela, Caracas, Venezuela. FAU - Charris, Jaime AU - Charris J AUID- ORCID: 0000-0003-4404-2619 AD - Laboratorio de Sintesis Organica, Facultad de Farmacia, Universidad Central de Venezuela, Caracas, Venezuela. FAU - Benaim, Gustavo AU - Benaim G AUID- ORCID: 0000-0002-9359-5546 AD - Unidad de Senalizacion Celular y Bioquimica de Parasitos, Instituto de Estudios Avanzados (IDEA), Caracas, Venezuela. AD - Laboratorio de Biofisica, Instituto de Biologia Experimental, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela. LA - eng GR - 20220PGP65/Fondo Nacional de Ciencia Tecnologia e Innovacion/ GR - Consejo de Desarrollo Cientifico y Humanistico, Universidad Central de Venezuela/ PT - Journal Article PL - Germany TA - ChemMedChem JT - ChemMedChem JID - 101259013 RN - 0 (Antiprotozoal Agents) SB - IM MH - *Leishmania mexicana/drug effects MH - Structure-Activity Relationship MH - Humans MH - *Antiprotozoal Agents/pharmacology/chemical synthesis/chemistry MH - Molecular Structure MH - Parasitic Sensitivity Tests MH - Animals MH - Dose-Response Relationship, Drug OTO - NOTNLM OT - acidocalcisomes OT - cytotoxicity OT - intracellular Ca2+ regulation OT - leishmaniasis OT - mitochondria OT - quinolines EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 16:23 PHST- 2026/10/05 00:00 [revised] PHST- 2026/06/12 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 16:23 [entrez] AID - e70550 [pii] AID - 10.1002/cmdc.70550 [doi] PST - ppublish SO - ChemMedChem. 2026 Nov 12;21(21):e70550. doi: 10.1002/cmdc.70550. PMID- 42956345 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1089-7690 (Electronic) IS - 0021-9606 (Linking) VI - 165 IP - 16 DP - 2026 Oct 28 TI - Chiral vibrational modes and vibrational circular dichroism. LID - 164133 [pii] LID - 10.1063/5.0353819 [doi] AB - The recent interest in chiral phonons in a variety of physical phenomena and their hypothesized role in the chiral-induced spin selectivity effect [Fransson, Phys. Rev. Res. 5, L022039 (2023)] call for further investigation into the chirality of molecular vibrations. Although molecular chirality has conventionally been quantified using structural properties, recent work has highlighted the role of dynamical response properties as chirality metrics. In this work, we examine an inter-atom helicity pseudoscalar as a complementary measure of vibrational chirality, associated with the vibrational circular dichroism (VCD) intensity in the fixed partial charge (FPC) approximation. This pseudoscalar is translationally and rotationally invariant, can distinguish between opposite enantiomers and, unlike the atomic pseudoscalar measure considered in our earlier work [Abraham and Nitzan, Phys. Rev. Lett. 133, 268001 (2024)], does not rely on a predefined symmetry axis. For a twisted ethane model as well as several small molecules, this pseudoscalar correlates well with structural descriptors based on the continuous chirality measure. Overall, our results support response-based metrics as a physically meaningful and practically useful characterization of vibrational chirality. Importantly, while the FPC-based VCD estimate provides a useful quantifier of vibrational chirality, we show that it is a rather poor predictor of the actual molecular VCD response because the latter is strongly influenced by the (vibrational configuration-dependent) molecular electronic response. CI - (c) 2026 Author(s). Published under an exclusive license by AIP Publishing. FAU - Tao, Xuecheng AU - Tao X AUID- ORCID: 0000-0003-2907-3839 AD - Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. FAU - Climent, Claudia AU - Climent C AUID- ORCID: 0000-0002-5302-1660 AD - Departament de Ciencia de Materials i Quimica Fisica and Institut de Quimica Teorica i Computacional (IQTCUB), Universitat de Barcelona, Marti i Franques 1, 08028 Barcelona, Spain. FAU - Abraham, Ethan AU - Abraham E AUID- ORCID: 0009-0009-9735-5839 AD - Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA. FAU - Feng, Jichen AU - Feng J AUID- ORCID: 0009-0005-5497-6943 AD - Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. FAU - Nitzan, Abraham AU - Nitzan A AUID- ORCID: 0000-0002-8431-0967 AD - Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. AD - Department of Physical Chemistry, Tel Aviv University, Tel Aviv 6997801, Israel. LA - eng PT - Journal Article PL - United States TA - J Chem Phys JT - The Journal of chemical physics JID - 0375360 SB - IM EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 16:22 PHST- 2026/07/24 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 16:22 [entrez] AID - 3407538 [pii] AID - 10.1063/5.0353819 [doi] PST - ppublish SO - J Chem Phys. 2026 Oct 28;165(16):164133. doi: 10.1063/5.0353819. PMID- 42956339 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1089-7690 (Electronic) IS - 0021-9606 (Linking) VI - 165 IP - 16 DP - 2026 Oct 28 TI - Generalized vibrational perturbation theory in curvilinear internal coordinates: Theory and applications. LID - 164128 [pii] LID - 10.1063/5.0355466 [doi] AB - Second-order vibrational perturbation theory (VPT2) is widely employed for the calculation of anharmonic vibrational properties thanks to its favorable balance between accuracy and computational cost. However, the presence of strong vibrational resonances requires dedicated treatments beyond standard perturbative approaches. In this work, a generalized VPT2 framework based on curvilinear internal coordinates is developed. Closed-form expressions are derived for the interaction terms associated with Fermi and Darling-Dennison resonances, including 1-1, 2-2, and 1-3 couplings. In contrast to conventional Cartesian-coordinate formulations, the present treatment accounts for additional kinetic-energy contributions arising from the non-diagonal form of the vibrational kinetic-energy operator. The resulting methodology has been implemented in a general computational engine and has been validated through applications to representative molecular systems. The importance of the variational treatment of resonances is highlighted through the investigation of selected case studies, while the flexibility of the proposed framework is demonstrated through its combination with dual-level schemes employing different electronic-structure methods for harmonic and anharmonic contributions. The present work extends the applicability of perturb-then-diagonalize approaches in curvilinear coordinates and provides an efficient route toward accurate anharmonic calculations for medium- and large-sized molecular systems. CI - (c) 2026 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). FAU - Mendolicchio, M AU - Mendolicchio M AUID- ORCID: 0000-0002-4504-853X AD - Scuola Normale Superiore, Piazza dei Cavalieri 7, I-56126 Pisa, Italy. LA - eng PT - Journal Article PL - United States TA - J Chem Phys JT - The Journal of chemical physics JID - 0375360 SB - IM EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 16:22 PHST- 2026/08/05 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 16:22 [entrez] AID - 3407532 [pii] AID - 10.1063/5.0355466 [doi] PST - ppublish SO - J Chem Phys. 2026 Oct 28;165(16):164128. doi: 10.1063/5.0355466. PMID- 42956336 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1089-7690 (Electronic) IS - 0021-9606 (Linking) VI - 165 IP - 16 DP - 2026 Oct 28 TI - Observable-specific kinetic-domain hierarchies in Markovian population dynamics. LID - 164125 [pii] LID - 10.1063/5.0357063 [doi] AB - Markovian population dynamics on a network are commonly formulated through a master equation for site populations and analyzed either in the site representation or in terms of independently evolving eigenmodes. Neither description directly resolves the relaxation of a selected observable into kinetically interpretable stages. We develop an observable-specific kinetic-domain (KD) representation that recasts this relaxation as a kinetic hierarchy. The resulting basis is algebraically equivalent to that obtained by Arnoldi orthogonalization of an observable-seeded Krylov sequence. This hierarchy describes the multidimensional population dynamics as a funnel-like sequence of kinetic stages with collective site-space structures and interstage transition rates. Generalized survival functions (GSFs) on nested kinetic subspaces then yield an exact stagewise decomposition of the time-dependent observable relaxation and, upon time integration, a characteristic passage time for each stage, thereby identifying the rate-determining stage and revealing the underlying relaxation mechanism. Applications to analytically solvable networks and extended two-dimensional lattices demonstrate how the KD/GSF framework resolves complex relaxation into interpretable collective stages and show that similar global relaxation time scales can conceal markedly different underlying sequences of kinetic steps. By extracting the collective kinetic pathway relevant to the selected observable, the KD representation complements site- and eigenmode-based descriptions and can enable dimensional reduction in large stochastic networks. CI - (c) 2026 Author(s). Published under an exclusive license by AIP Publishing. FAU - Yang, Mino AU - Yang M AUID- ORCID: 0000-0001-9504-0280 AD - Department of Chemistry, Chungbuk National University, Cheongju, Chungbuk 28644, South Korea. LA - eng PT - Journal Article PL - United States TA - J Chem Phys JT - The Journal of chemical physics JID - 0375360 SB - IM EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 16:22 PHST- 2026/08/18 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 16:22 [entrez] AID - 3407529 [pii] AID - 10.1063/5.0357063 [doi] PST - ppublish SO - J Chem Phys. 2026 Oct 28;165(16):164125. doi: 10.1063/5.0357063. PMID- 42956330 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1860-7187 (Electronic) IS - 1860-7179 (Print) IS - 1860-7179 (Linking) VI - 21 IP - 21 DP - 2026 Nov 12 TI - Integration of Chemical Probes and Degradomics Reveals That Cathepsin B Functions Primarily as a Dipeptidyl Carboxypeptidase in Macrophages. LID - e70530 [pii] LID - 10.1002/cmdc.70530 [doi] AB - Cathepsin B (Ctsb) is a lysosomal cysteine protease implicated in proteostasis and disease, but its endogenous substrate specificity and activity in intact cells remain incompletely defined. Here, we applied an integrated chemical biology and degradomics approach to profile Ctsb function in RAW264.7 macrophages using the irreversible inhibitor Z-RK-AOMK. Z-RK-AOMK treatment effectively inhibited intracellular Ctsb activity while also perturbing the broader cysteine cathepsin network, including altered activities of Ctsx and Ctsl, and accumulation of an active higher molecular weight Ctss species consistent with altered Ctss maturation. Despite these changes in cysteine cathepsin activity, Z-RK-AOMK treatment produced limited changes in global protein abundance and N-terminal processing. In contrast, C-terminomic profiling revealed enrichment of cleavage events occurring two and four residues upstream of native protein C-termini. This pattern supports a predominant role for Ctsb as a dipeptidyl carboxypeptidase in cells and highlights C-terminome profiling as a sensitive functional readout of its activity. Finally, we developed a fluorescent activity-based probe, BODIPY-RK-AOMK, which selectively labels active Ctsb in cell lysates and live cells, enabling visualization of its predominantly lysosomal localization. Together, these findings define the cellular processing signature of Ctsb in macrophages and establish a complementary activity-based probe for mapping its activity in biological systems. CI - (c) 2026 The Author(s). ChemMedChem published by Wiley-VCH GmbH. FAU - Yek, Kai-Qi AU - Yek KQ AUID- ORCID: 0000-0002-0670-8195 AD - Department of Biochemistry & Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia. FAU - Apolloni, Francesca L AU - Apolloni FL AD - Department of Biochemistry & Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia. FAU - Xu, Bangyan AU - Xu B AD - Department of Biochemistry & Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia. AD - Molecular Imaging and Nano Therapeutics Laboratory, Baker Heart and Diabetes Institute, Melbourne, VIC, Australia. AD - Centre for Cardiometabolic mRNA Therapy, Baker Heart and Diabetes Institute, Melbourne, VIC, Australia. AD - Department of Cardiometabolic Health, University of Melbourne, Parkville, VIC, Australia. FAU - Nguyen, Nghi H AU - Nguyen NH AD - The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia. AD - Department of Medical Biology, The University of Melbourne, Parkville, VIC, Australia. FAU - Ziegler, Alexander R AU - Ziegler AR AUID- ORCID: 0000-0003-0759-9848 AD - Department of Biochemistry & Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia. FAU - Scott, Nichollas E AU - Scott NE AUID- ORCID: 0000-0003-2556-8316 AD - Department of Microbiology & Immunology, Peter Doherty Institute, The University of Melbourne, Parkville, VIC, Australia. FAU - Sleebs, Brad E AU - Sleebs BE AUID- ORCID: 0000-0001-9117-1048 AD - The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia. AD - Department of Medical Biology, The University of Melbourne, Parkville, VIC, Australia. FAU - Edgington-Mitchell, Laura E AU - Edgington-Mitchell LE AUID- ORCID: 0000-0002-6810-6149 AD - Department of Biochemistry & Pharmacology, Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Parkville, VIC, Australia. LA - eng GR - 2011119/National Health and Medical Research Council/ PT - Journal Article PL - Germany TA - ChemMedChem JT - ChemMedChem JID - 101259013 RN - EC 3.4.22.1 (Cathepsin B) RN - 0 (Molecular Probes) RN - 0 (Fluorescent Dyes) RN - 0 (Fluorescent Chemosensor Compounds) SB - IM MH - *Cathepsin B/metabolism/antagonists & inhibitors MH - Animals MH - Mice MH - *Macrophages/metabolism/drug effects/enzymology MH - RAW 264.7 Cells MH - *Molecular Probes/chemistry/pharmacology MH - Molecular Structure MH - *Fluorescent Dyes/chemistry/pharmacology MH - Structure-Activity Relationship MH - Fluorescent Chemosensor Compounds PMC - PMC13719178 OTO - NOTNLM OT - C-terminomics OT - activity-based probes OT - cathepsin B OT - dipeptidyl carboxypeptidase OT - proteases COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 PMCR- 2026/10/29 CRDT- 2026/10/29 16:12 PHST- 2026/09/17 00:00 [revised] PHST- 2026/07/31 00:00 [received] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 16:12 [entrez] PHST- 2026/10/29 00:00 [pmc-release] AID - e70530 [pii] AID - CMDC70530 [pii] AID - 10.1002/cmdc.70530 [doi] PST - ppublish SO - ChemMedChem. 2026 Nov 12;21(21):e70530. doi: 10.1002/cmdc.70530. PMID- 42956329 OWN - NLM STAT- Publisher LR - 20261029 IS - 1537-453X (Electronic) IS - 0277-3732 (Linking) DP - 2026 Oct 30 TI - Discovery of Promising USP21 Small Molecule Inhibitors: A Computational and Artificial Intelligence Modeling-Based Drug Repurposing Study for Non-Small Cell Lung Cancer. LID - 10.1097/COC.0000000000001391 [doi] AB - OBJECTIVES: Ubiquitin specific protease 21 (USP21) is considered a novel lung cancer protein target. Research has revealed that USP21 is highly expressed in non-small cell lung cancer and its inhibition leads to decreased levels of the immune checkpoint inhibitor programmed death ligand 1 (PD-L1). Hence, targeting the USP21/ PD-L-1 interaction is a highly sought after therapeutic strategy. METHODS: In this study, we applied an integrated methodology beginning with the refinement and optimization of the USP21 crystal structure via a novel computational and artificial intelligence- based modeling strategy. Our highly predicted chemical structure was used for screening of 6877 molecules, including investigational and FDA-approved drugs, along with a known potent USP21 inhibitor. We then performed 10 ns molecular dynamics (MD) simulations for select molecules, followed by 100 ns MD simulations for 29 lead molecules, along with post-MD MM/GBSA system free energy DeltaG calculations. RESULTS: Our comprehensive analysis led to the discovery of 7 molecules as the most promising USP21 inhibitors. These drugs are deslorelin, dotatate, vedroprevir, vasopressin, pentagastrin, furaprevir, and bleomycin, which is a currently approved anticancer drug used for the treatment of a variety of cancer types. CONCLUSIONS: Our results represent a strong scaffold that can be used by scientists, researchers, and chemists, opening the route for further drug design and discovery research. Drug repurposing offers a novel approach to identify potential cancer therapeutics and has the potential of making a significant impact on reducing the financial toxicity associated with oncology treatments for our patients. CI - Copyright (c) 2026 Wolters Kluwer Health, LLC. All rights reserved. FAU - Kanan, Tarek AU - Kanan T AD - Division of Pulmonary, Critical Care and Sleep Medicine, Houston Methodist, TX. FAU - Kanan, Duaa AU - Kanan D AD - Division of Hematology and Medical Oncology, University of Vermont Medical Center, VT. FAU - Kandula, Manasa AU - Kandula M AD - Department of Internal Medicine, University of Illinois College of Medicine, Peoria, IL. LA - eng PT - Journal Article DEP - 20261030 PL - United States TA - Am J Clin Oncol JT - American journal of clinical oncology JID - 8207754 SB - IM OTO - NOTNLM OT - NSCLC OT - USP21 OT - drug design OT - novel therapeutics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 16:12 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 16:12 [entrez] AID - 00000421-990000000-00469 [pii] AID - 10.1097/COC.0000000000001391 [doi] PST - aheadofprint SO - Am J Clin Oncol. 2026 Oct 30. doi: 10.1097/COC.0000000000001391. PMID- 42956328 OWN - NLM STAT- Publisher LR - 20261029 IS - 1944-8252 (Electronic) IS - 1944-8244 (Linking) DP - 2026 Oct 30 TI - Microneedle-Based Oral Mucosal Vaccines: Mechanisms, Challenges, and Emerging Strategies. LID - 10.1021/acsami.6c16754 [doi] AB - Oral mucosal vaccination is a minimally invasive and highly acceptable strategy that can trigger both local and systemic immunity. However, its progress toward clinical use is hindered by salivary washout, the multilayered epithelium, and a tolerance-prone immune environment. Microneedles offer a promising way to overcome these barriers. They deliver antigens beneath the oral surface, place them directly in immune-responsive tissues, and can remain operational in the dynamic, enzyme-rich oral cavity. Recent research shows that microneedles enable precise antigen deposition, stabilize fragile vaccine components, and provide controllable release patterns. Studies using both model antigens and clinically relevant targets suggest that oral microneedle vaccination can induce systemic IgG, mucosal sIgA, and tissue-resident cellular responses. Distal mucosal immune responses have also been observed. These distal responses are consistent with the common mucosal immune system (CMIS), although direct evidence of CMIS-dependent lymphocyte trafficking remains limited. The co-delivery of immunostimulatory materials, adjuvants, or nanoparticle carriers further enhances antigen presentation and helps counteract oral immune tolerance. Despite these advances, several challenges remain. These include clarifying the early immune events in oral tissues, identifying adjuvants suited to the oral environment, and ensuring safety, stability, and user acceptability for clinical translation. Overall, current evidence positions microneedles as a versatile and promising platform for mucosal vaccination. Continued integration of oral immunology with formulation and microneedle design will be essential for moving these systems toward real-world application. CI - (c) 2026 American Chemical Society. FAU - Hu, Ouqian AU - Hu O AD - State Key Laboratory of Oral Diseases and National Center for Stomatology and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan610041, China. FAU - Huang, Linyu AU - Huang L AD - State Key Laboratory of Oral Diseases and National Center for Stomatology and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan610041, China. FAU - Huang, Lixuan AU - Huang L AD - State Key Laboratory of Oral Diseases and National Center for Stomatology and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan610041, China. FAU - Yang, Jingru AU - Yang J AD - State Key Laboratory of Oral Diseases and National Center for Stomatology and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan610041, China. FAU - Tang, Yufei AU - Tang Y AD - State Key Laboratory of Oral Diseases and National Center for Stomatology and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan610041, China. FAU - Pan, Shulei AU - Pan S AUID- ORCID: 0009-0007-5640-5727 AD - Institute of Biopharmaceuticals, West China Hospital, Sichuan University, Chengdu, Sichuan610041, China. FAU - Pu, Meng AU - Pu M AD - Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas77030, United States. FAU - Xu, Junhua AU - Xu J AD - Institute of Biopharmaceuticals, West China Hospital, Sichuan University, Chengdu, Sichuan610041, China. FAU - Xiang, Lin AU - Xiang L AUID- ORCID: 0000-0002-7938-5112 AD - State Key Laboratory of Oral Diseases and National Center for Stomatology and National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan610041, China. LA - eng GR - 32200768/National Natural Science Foundation of China/ GR - 82170997/National Natural Science Foundation of China/ GR - 82370996/National Natural Science Foundation of China/ GR - 82571134/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261030 PL - United States TA - ACS Appl Mater Interfaces JT - ACS applied materials & interfaces JID - 101504991 SB - IM OTO - NOTNLM OT - antigen delivery systems OT - controlled release OT - microneedles OT - mucosal immunity OT - oral mucosa OT - vaccine adjuvants EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 16:03 PHST- 2026/08/13 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 16:03 [entrez] AID - 5461152 [pii] AID - 10.1021/acsami.6c16754 [doi] PST - aheadofprint SO - ACS Appl Mater Interfaces. 2026 Oct 30. doi: 10.1021/acsami.6c16754. PMID- 42956324 OWN - NLM STAT- Publisher LR - 20261029 IS - 1527-7755 (Electronic) IS - 0732-183X (Linking) DP - 2026 Oct 29 TI - Therapy for Stage IV Non-Small Cell Lung Cancer With Driver Alterations: ASCO Living Guideline, Version 2026.3.4. PG - 101200JCO2602597 LID - 10.1200/JCO-26-02597 [doi] AB - [Box: see text]Living guidelines are developed for selected topic areas with rapidly evolving evidence that drives frequent change in recommended clinical practice. Living guidelines are updated on a regular schedule by a standing expert panel that systematically reviews the health literature on a continuous basis, as described in the ASCO Guidelines Methodology Manual. ASCO Living Guidelines follow the ASCO Conflict of Interest Policy Implementation for Clinical Practice Guidelines. Living Guidelines and updates are not intended to substitute for independent professional judgment of the treating clinician and do not account for individual variation among patients. See appendix for disclaimers and other important information (Appendix I and Appendix II). Updates are published regularly and can be found on the ASCO Publications website. FAU - Reuss, Joshua AU - Reuss J AUID- ORCID: 0000-0002-6401-9080 AD - Georgetown University, Washington, DC, US. FAU - Ismaila, Nofisat AU - Ismaila N AUID- ORCID: 0000-0003-2074-1896 AD - American Society of Clinical Oncology (ASCO), Alexandria, VA, US. FAU - Doroshow, Deborah B AU - Doroshow DB AUID- ORCID: 0000-0002-2221-9818 AD - Tisch Cancer Center, Icahn School of Medicine at Mount Sinai, New York, NY. FAU - Feldman, Jill AU - Feldman J AUID- ORCID: 0000-0002-2107-8240 AD - EGFR Resisters patient advocacy group, Deerfield, IL, US. FAU - MacVicar, Gary R AU - MacVicar GR AUID- ORCID: 0009-0007-2185-6368 AD - Illinois CancerCare, Peoria, IL, US. FAU - Noronha, Vanita AU - Noronha V AUID- ORCID: 0000-0002-0191-2744 AD - Tata Memorial Centre, India. FAU - Saltos, Andreas AU - Saltos A AUID- ORCID: 0000-0003-3622-026X AD - Moffitt Cancer Center, Tampa, FL. FAU - Scott, Susan C AU - Scott SC AUID- ORCID: 0000-0002-1797-1685 AD - Johns Hopkins School of Medicine, Washington, DC. FAU - Singhi, Eric K AU - Singhi EK AD - University of Texas MD Anderson Cancer Center, Houston, TX, US. FAU - Suga, Jennifer Marie AU - Suga JM AD - Kaiser Permanente Vallejo Medical Center, Vallejo, CA, US. FAU - Bazhenova, Lyudmila AU - Bazhenova L AUID- ORCID: 0000-0001-8764-4359 AD - University of California, San Diego, San Diego, CA, US. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Clin Oncol JT - Journal of clinical oncology : official journal of the American Society of Clinical Oncology JID - 8309333 SB - IM EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 16:03 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 16:03 [entrez] AID - 10.1200/JCO-26-02597 [doi] PST - aheadofprint SO - J Clin Oncol. 2026 Oct 29:101200JCO2602597. doi: 10.1200/JCO-26-02597. PMID- 42956319 OWN - NLM STAT- Publisher LR - 20261029 IS - 2576-6422 (Electronic) IS - 2576-6422 (Linking) DP - 2026 Oct 29 TI - Photothermal Antibacterial Performance of Bio-Derived Polymer Composite Materials. LID - 10.1021/acsabm.6c01495 [doi] AB - In this study, we utilize a controlled cellulose nanofiber (CNF) platform to investigate how synthesis parameters of delaminated 2D MXene components influence cellular response, photothermal behavior, and antimicrobial activity of the resulting composite materials. Six Ti3C2Tx MXenes were synthesized, delaminated, and then integrated into a cellulose nanofiber network under identical loading and vacuum-filtration conditions. The resulting free-standing CNF-MXene composites supported metabolic activity over six days and, upon near-infrared irradiation, rapidly increased in temperature and effectively reduced planktonic and biofilm-associated Staphylococcus aureus. Importantly, antibacterial performance varied substantially among MXenes and could not be predicted from the final temperature reached after 120 sec of irradiation alone. The MXene synthesis and delamination route therefore emerges as an upstream design variable that simultaneously modifies surface composition and Ti chemical-state distribution, flake morphology, and photothermal kinetics, producing distinct antibacterial responses within an otherwise fixed CNF composite platform. These results establish a processing-structure-property relationship for rationally selecting MXene synthesis routes for embedding in externally activated antibacterial composites and demonstrate that nominally identical Ti3C2Tx phases cannot be treated as interchangeable functional nanostructures for biomedical applications. CI - (c) 2026 The Author(s). Published by American Chemical Society. FAU - Poliukhova, Valeriia AU - Poliukhova V AUID- ORCID: 0000-0002-3491-9328 AD - School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, Georgia30332, United States. FAU - Korniienko, Viktoriia AU - Korniienko V AD - Biomedical Research Center, Medical Institute, Sumy State University, 31 Sanatornaya Str., Sumy40018, Ukraine. AD - Institute of Atomic Physics and Spectroscopy, University of Latvia, 3 Jelgavas Str., RigaLV-1004, Latvia. FAU - Lee, Jaejun AU - Lee J AUID- ORCID: 0000-0002-9745-1048 AD - School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, Georgia30332, United States. FAU - Baginskiy, Ivan AU - Baginskiy I AUID- ORCID: 0000-0002-0092-9018 AD - Biomedical Research Center, Medical Institute, Sumy State University, 31 Sanatornaya Str., Sumy40018, Ukraine. AD - Y-Carbon LLC , Kyiv04116, Ukraine. FAU - Dukhnovskyi, Serhii AU - Dukhnovskyi S AD - Biomedical Research Center, Medical Institute, Sumy State University, 31 Sanatornaya Str., Sumy40018, Ukraine. AD - Y-Carbon LLC , Kyiv04116, Ukraine. FAU - Zhang, Yiming AU - Zhang Y AUID- ORCID: 0009-0003-5914-7357 AD - School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, Georgia30332, United States. FAU - Brackenridge, Justin AU - Brackenridge J AUID- ORCID: 0009-0000-4792-7338 AD - School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, Georgia30332, United States. FAU - Shubin, Pavlo AU - Shubin P AUID- ORCID: 0000-0002-5477-1914 AD - Institute of Atomic Physics and Spectroscopy, University of Latvia, 3 Jelgavas Str., RigaLV-1004, Latvia. FAU - Diedkova, Kateryna AU - Diedkova K AD - Biomedical Research Center, Medical Institute, Sumy State University, 31 Sanatornaya Str., Sumy40018, Ukraine. AD - Institute of Atomic Physics and Spectroscopy, University of Latvia, 3 Jelgavas Str., RigaLV-1004, Latvia. FAU - Zahorodna, Veronika AU - Zahorodna V AUID- ORCID: 0000-0002-8873-015X AD - Y-Carbon LLC , Kyiv04116, Ukraine. AD - NanoCarbonTech , Rubiez 46, Poznan61-612, Poland. FAU - Gogotsi, Oleksiy AU - Gogotsi O AUID- ORCID: 0000-0002-3173-4432 AD - Biomedical Research Center, Medical Institute, Sumy State University, 31 Sanatornaya Str., Sumy40018, Ukraine. AD - Y-Carbon LLC , Kyiv04116, Ukraine. AD - NanoCarbonTech , Rubiez 46, Poznan61-612, Poland. FAU - Pogorielov, Maksym AU - Pogorielov M AUID- ORCID: 0000-0001-9372-7791 AD - Biomedical Research Center, Medical Institute, Sumy State University, 31 Sanatornaya Str., Sumy40018, Ukraine. AD - Institute of Atomic Physics and Spectroscopy, University of Latvia, 3 Jelgavas Str., RigaLV-1004, Latvia. AD - NanoCarbonTech , Rubiez 46, Poznan61-612, Poland. FAU - Tsukruk, Vladimir V AU - Tsukruk VV AUID- ORCID: 0000-0001-5489-0967 AD - School of Materials Science and Engineering, Georgia Institute of Technology, Atlanta, Georgia30332, United States. LA - eng GR - 101131147/HORIZON EUROPE Marie Sklodowska-Curie Actions/ PT - Journal Article DEP - 20261029 PL - United States TA - ACS Appl Bio Mater JT - ACS applied bio materials JID - 101729147 SB - IM OTO - NOTNLM OT - Staphylococcus aureus OT - Ti3C2Tx MXene OT - cellulose nanofibers OT - natural polymers OT - photothermal antibacterial materials OT - surface chemistry OT - wound-contacting films EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 16:02 PHST- 2026/07/16 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 16:02 [entrez] AID - 5461184 [pii] AID - 10.1021/acsabm.6c01495 [doi] PST - aheadofprint SO - ACS Appl Bio Mater. 2026 Oct 29. doi: 10.1021/acsabm.6c01495. PMID- 42956314 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1439-7641 (Electronic) IS - 1439-4235 (Linking) VI - 27 IP - 21 DP - 2026 Nov 12 TI - Spectroscopic Characterization of Mass-Selected Heteronuclear Iron-Silver Carbonyl Cluster Anions. LID - e70615 [pii] LID - 10.1002/cphc.70615 [doi] AB - Heteronuclear iron-silver carbonyl anions AgFe(CO)(n) (-) (n = 4-6) are produced via a laser vaporization supersonic cluster ion source and are studied by infrared photodissociation spectroscopy in the carbonyl stretching region. Their structures are determined by comparing experimental spectra with density-functional theory calculations. The experimentally observed AgFe(CO)(n) (-) (n = 4-5) anions possess (OC)(4)Fe-Ag(CO)(n-4) structures, featuring a formal Fe-Ag sigma-type bond and an 18-electron Fe(CO)(4) (-) core. AgFe(CO)(6) (-) is a CO-tagged adduct with a physisorbed CO coexisting with a chemisorbed Ag-CO. Bonding analysis reveals multicenter electron-sharing sigma character. The AgCO unit in AgFe(CO)(5) (-) retains a distinctly bent geometry, owing to silver's large 4d-5s energy separation, which quenches pi backdonation, and to residual Ag 5s density that repels the CO 5sigma lone pair. These factors also weaken the Ag-CO bond and limit CO saturation to n = 5. CI - (c) 2026 Wiley-VCH GmbH. FAU - Cai, Yan AU - Cai Y AD - Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, School of Materials Science and Chemical Engineering, Institute of Mass Spectrometry, Ningbo University, Ningbo, Zhejiang, China. FAU - Meng, Luyan AU - Meng L AD - Ningbo No.2 Hospital, Ningbo, Zhejiang, China. AD - School of Chemistry and Materials Science, East China University of Technology, Nanchang, Jiangxi, China. FAU - Chi, Chaoxian AU - Chi C AUID- ORCID: 0000-0003-3357-8741 AD - Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, School of Materials Science and Chemical Engineering, Institute of Mass Spectrometry, Ningbo University, Ningbo, Zhejiang, China. LA - eng GR - 22473065/National Natural Science Foundation of China/ PT - Journal Article PL - Germany TA - Chemphyschem JT - Chemphyschem : a European journal of chemical physics and physical chemistry JID - 100954211 SB - IM OTO - NOTNLM OT - bonding character OT - heteronuclear transition metal carbonyl cluster OT - infrared photodissociation spectroscopy OT - iron carbonyl OT - silver carbonyl OT - theoretical calculation EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 15:53 PHST- 2026/10/08 00:00 [revised] PHST- 2026/08/31 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 15:53 [entrez] AID - e70615 [pii] AID - 10.1002/cphc.70615 [doi] PST - ppublish SO - Chemphyschem. 2026 Nov 12;27(21):e70615. doi: 10.1002/cphc.70615. PMID- 42956312 OWN - NLM STAT- Publisher LR - 20261029 IS - 1809-452X (Electronic) IS - 1516-4446 (Linking) DP - 2026 Oct 20 TI - Thirty Years of the Laboratory of Panic and Respiration: From Respiratory Panic to Translational Psychiatry. LID - 10.47626/1516-4446-2026-5265 [doi] FAU - Dutra, Pablo E P AU - Dutra PEP AD - Laboratorio de Panico e Respiracao (LABPR), Instituto de Psiquiatria (IPUB), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil. Laboratorio de Historia da Psiquiatria, Neurologia e Saude Mental (HiPsiNS), IPUB, UFRJ, Rio de Janeiro, RJ, Brazil. FAU - Horato, Natia AU - Horato N AD - Laboratorio de Panico e Respiracao (LABPR), Instituto de Psiquiatria (IPUB), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil. Laboratorio de Historia da Psiquiatria, Neurologia e Saude Mental (HiPsiNS), IPUB, UFRJ, Rio de Janeiro, RJ, Brazil. FAU - Quagliato, Laiana AU - Quagliato L AD - Laboratorio de Panico e Respiracao (LABPR), Instituto de Psiquiatria (IPUB), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil. Laboratorio de Historia da Psiquiatria, Neurologia e Saude Mental (HiPsiNS), IPUB, UFRJ, Rio de Janeiro, RJ, Brazil. Laboratorio de Psiquiatria da Infancia e Adolescencia, IPUB, UFRJ, Rio de Janeiro, RJ, Brazil. FAU - Gomes, Marleide da Mota AU - Gomes MDM AD - Laboratorio de Historia da Psiquiatria, Neurologia e Saude Mental (HiPsiNS), IPUB, UFRJ, Rio de Janeiro, RJ, Brazil. Instituto de Neurologia Deolindo Couto (INDC), UFRJ, Rio de Janeiro, RJ, Brazil. FAU - Nardi, Antonio E AU - Nardi AE AD - Laboratorio de Panico e Respiracao (LABPR), Instituto de Psiquiatria (IPUB), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro, RJ, Brazil. Laboratorio de Historia da Psiquiatria, Neurologia e Saude Mental (HiPsiNS), IPUB, UFRJ, Rio de Janeiro, RJ, Brazil. LA - eng PT - Letter DEP - 20261020 PL - Brazil TA - Braz J Psychiatry JT - Revista brasileira de psiquiatria (Sao Paulo, Brazil : 1999) JID - 100895975 SB - IM COIS- Antonio E. Nardi is Editor-in-Chief, Laiana Quagliato is an Associate Editor, and Natia Horato is an Assistant Editor of the Brazilian Journal of Psychiatry. Pablo E. P. Dutra is a doctoral candidate at the Federal University of Rio de Janeiro under the supervision of Antonio E. Nardi and Laiana Quagliato. These academic and editorial relationships are disclosed for transparency. Antonio E. Nardi, Laiana Quagliato, and Natia Horato will have no involvement in the editorial assessment, reviewer selection, peer-review process, or decision-making concerning this manuscript, which should be handled independently by an editor without a conflict of interest. No other conflicts of interest were reported. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 15:43 PHST- 2026/09/27 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 15:43 [entrez] AID - 10.47626/1516-4446-2026-5265 [doi] PST - aheadofprint SO - Braz J Psychiatry. 2026 Oct 20. doi: 10.47626/1516-4446-2026-5265. PMID- 42956305 OWN - NLM STAT- Publisher LR - 20261029 IS - 2767-9764 (Electronic) IS - 2767-9764 (Linking) DP - 2026 Oct 29 TI - Mechanisms of pregnancy-associated triple negative breast cancer risk: insights from spatial omics. LID - 10.1158/2767-9764.CRC-26-0326 [doi] AB - Pregnancy-associated triple-negative breast cancer (PA-TNBC) is a highly aggressive, hard-to-treat malignancy, with increased risk linked to post-lactational mammary gland involution. To understand this, we used GeoMx Digital Spatial Profiler to analyze the transcriptional landscape of 46 treatment-naive PA-TNBC tissues from 33 women (10 pre-involution/PRE; 23 post-involution/POST, diagnosed within 3 years of delivery), focusing on epithelial and microenvironmental changes. Our findings reveal that the most significant transcriptional differences between PRE and POST exist in non-invasive epithelia and during the transition to invasion. POST non-invasive epithelia showed distinct activation of inflammatory and developmental pathways, while transitioning to TNBC involved elevated chromatin remodeling and migration. Notably, the tumor microenvironment (TME) surrounding POST invasive epithelia featured the greatest immune cell infiltration yet also increased immune exhaustion. Pseudotime analysis highlighted the 1-2 year postpartum window as having the strongest inflammatory signaling. These results underline the need for early molecular detection in normal-appearing post-involution tissues and suggest that targeting the TME is a viable strategy for treating POST PA-TNBC. FAU - Veraksa, Darya AU - Veraksa D AUID- ORCID: 0009-0001-9320-180X AD - University of California San Diego La Jolla, California United States. ROR: https://ror.org/0168r3w48 FAU - Mukund, Kavitha AU - Mukund K AUID- ORCID: 0000-0002-3570-2315 AD - University of California San Diego La Jolla, California United States. ROR: https://ror.org/0168r3w48 FAU - Frankhouser, David E AU - Frankhouser DE AUID- ORCID: 0000-0002-8233-5853 AD - City Of Hope National Medical Center Duarte, CA United States. ROR: https://ror.org/00w6g5w60 FAU - Yang, Lixin AU - Yang L AUID- ORCID: 0000-0002-9429-6580 AD - City of Hope Duarte, CA United States. ROR: https://ror.org/01z1vct10 FAU - Tomsic, Jerneja AU - Tomsic J AUID- ORCID: 0009-0007-0927-0428 AD - City of Hope Duarte, CA United States. ROR: https://ror.org/01z1vct10 FAU - Pillai, Raju K AU - Pillai RK AUID- ORCID: 0000-0001-8553-4252 AD - City Of Hope National Medical Center Los Angeles, California United States. ROR: https://ror.org/00w6g5w60 FAU - Venkatasubramani, Jeyasri AU - Venkatasubramani J AUID- ORCID: 0009-0008-0888-6527 AD - University of California San Diego La Jolla, California United States. ROR: https://ror.org/0168r3w48 FAU - Schmolze, Daniel AU - Schmolze D AUID- ORCID: 0000-0002-6947-2546 AD - City of Hope Comprehensive Cancer Center Duarte, CA United States. FAU - Wu, Xiao-Cheng AU - Wu XC AUID- ORCID: 0000-0003-3663-5027 AD - Louisiana State University Health Sciences Center New Orleans New Orleans, Louisiana United States. ROR: https://ror.org/01qv8fp92 FAU - LeBlanc, Mary Anne AU - LeBlanc MA AUID- ORCID: 0009-0006-6504-7928 AD - Louisiana State University Health Sciences Center New Orleans New Orleans, Louisiana United States. ROR: https://ror.org/01qv8fp92 FAU - Miele, Lucio AU - Miele L AUID- ORCID: 0000-0002-5853-7287 AD - Louisiana State University Health Sciences Center New Orleans New Orleans, LA United States. ROR: https://ror.org/01qv8fp92 FAU - Ochoa, Augusto C AU - Ochoa AC AUID- ORCID: 0000-0001-6457-8029 AD - Louisiana State University Health Sciences Center New Orleans, LA United States. FAU - Seewaldt, Victoria L AU - Seewaldt VL AUID- ORCID: 0000-0002-7289-9268 AD - City of Hope Duarte, CA United States. ROR: https://ror.org/01z1vct10 FAU - Subramaniam, Shankar AU - Subramaniam S AUID- ORCID: 0000-0002-8059-4659 AD - University of California San Diego La Jolla, CA United States. ROR: https://ror.org/0168r3w48 LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Cancer Res Commun JT - Cancer research communications JID - 9918281580506676 SB - IM EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 15:42 PHST- 2026/10/28 00:00 [accepted] PHST- 2026/05/04 00:00 [received] PHST- 2026/09/07 00:00 [revised] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 15:42 [entrez] AID - 789727 [pii] AID - 10.1158/2767-9764.CRC-26-0326 [doi] PST - aheadofprint SO - Cancer Res Commun. 2026 Oct 29. doi: 10.1158/2767-9764.CRC-26-0326. PMID- 42956285 OWN - NLM STAT- Publisher LR - 20261029 IS - 1744-8379 (Electronic) IS - 1473-7167 (Linking) DP - 2026 Oct 29 TI - Medication adherence in clinical trials: measurement methods, methodological challenges, and reporting standards. PG - 1-15 LID - 10.1080/14737167.2026.2754563 [doi] AB - INTRODUCTION: Medication adherence is a critical determinant of therapeutic exposure, efficacy estimation, and safety evaluation in clinical trials. Despite its fundamental role, adherence remains inconsistently defined, measured, and reported across the clinical trial landscape. AREAS COVERED: This review evaluates contemporary conceptual frameworks and measurement strategies for medication adherence in clinical trials. It examines the implications of suboptimal adherence for trial design, statistical analysis, and the interpretation of therapeutic outcomes, while addressing the impact on both internal and external validity. EXPERT OPINION: There is a necessary transition from conventional, binary models of 'compliance' to the multidimensional ABC taxonomy (initiation, implementation, discontinuation and persistence), which characterizes adherence as a dynamic, longitudinal process. Traditional assessment methods - such as self-reports, pill counts, and pharmacy refill metrics - vary in feasibility but are often limited by recall bias and poor temporal resolution. Conversely, electronic monitoring and digitally enabled technologies provide more objective, high-resolution proxy measures that are essential for better characterizing real-world medication-taking behavior. However, persistent methodological heterogeneity and a lack of standardized reporting undermine the comparability of trial results. The adoption of harmonized reporting standards, specifically the EMERGE guidelines, and the prospective integration of digital adherence assessment is vital to strengthening the evidentiary integrity of future therapeutic evidence. FAU - Nogueiras-Alvarez, Rita AU - Nogueiras-Alvarez R AUID- ORCID: 0000-0001-7989-6951 AD - Clinical Pharmacology, Osakidetza-Basque Health Service, Galdakao-Usansolo University Hospital, Galdakao, Spain. FAU - Roncato, Rossana AU - Roncato R AUID- ORCID: 0000-0002-4219-6182 AD - Department of Medicine, University of Udine, Udine, Italy. FAU - Stanic Benic, Mirjana AU - Stanic Benic M AUID- ORCID: 0000-0002-4123-9937 AD - Division of Gastroenterology, Endocrinology and Diabetology, Department of Internal Medicine, General Hospital Dr. Josip Bencevic, Slavonski Brod, Croatia. FAU - Maleskic Kapo, Sanita AU - Maleskic Kapo S AUID- ORCID: 0000-0002-9654-3928 AD - Institute for Pharmacology, Toxicology and Clinical Pharmacology, University of Sarajevo - Medical Faculty, Sarajevo, Bosnia and Herzegovina. FAU - Potocnjak, Ines AU - Potocnjak I AUID- ORCID: 0000-0001-9351-9669 AD - Sestre Milosrdnice University Hospital Center, Zagreb, Croatia. AD - School of Medicine Catholic University of Croatia, Zagreb, Croatia. FAU - Belancic, Andrej AU - Belancic A AUID- ORCID: 0000-0001-7848-6600 AD - Department of Basic and Clinical Pharmacology and Toxicology, Faculty of Medicine, University of Rijeka, Rijeka, Croatia. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - Expert Rev Pharmacoecon Outcomes Res JT - Expert review of pharmacoeconomics & outcomes research JID - 101132257 SB - IM OAB - When new medicines are evaluated in clinical trials, it is essential to know whether participants are taking their medication exactly as prescribed. If participants miss doses or stop treatment early, researchers might incorrectly conclude that a medicine is ineffective or unsafe, when in reality the drug was simply not taken properly. Historically, trials relied on basic methods like counting remaining pills or asking patients directly; however, these methods can be inaccurate because people may forget or unintentionally misreport their dosing habits. This review explains how clinical trials can better measure medication use by treating adherence as a dynamic, longitudinal process. We evaluate modern measurement tools, including electronic packaging (such as smart pill bottles that record when they are opened) and digital sensors, which provide detailed, time-stamped information. Because no single tool fits every study, we emphasize that researchers must select 'fit-for-purpose' methods tailored to the specific trial. Finally, adopting standardized reporting guidelines (such as the EMERGE standards) will ensure that clinical trial findings are transparent, reliable, and accurately reflect how medicines work in real-world healthcare. OABL- eng OTO - NOTNLM OT - Assessment of medication adherence OT - clinical pharmacology OT - clinical trials OT - measurement of medication adherence OT - therapeutic adherence and compliance EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 15:23 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 15:23 [entrez] AID - 10.1080/14737167.2026.2754563 [doi] PST - aheadofprint SO - Expert Rev Pharmacoecon Outcomes Res. 2026 Oct 29:1-15. doi: 10.1080/14737167.2026.2754563. PMID- 42956273 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1521-4184 (Electronic) IS - 0365-6233 (Linking) VI - 359 IP - 11 DP - 2026 Nov TI - Effective Cross-Coupling Synthesis of Novel Ethynyl-Decorated Benzo[b][1,6]naphthyridines and Their Evaluation as Inhibitors of Cholinesterases and Monoamine Oxidases. LID - e70373 [pii] LID - 10.1002/ardp.70373 [doi] AB - An effective oxidative ethynylation of 1,2,3,4-tetrahydrobenzo[b][1,6]naphthyridines (THBNs) was developed. A practical improvement was achieved by optimizing a co-catalytic system composed of O(2), CuI, and DMAP. The co-catalytic system allows a decrease in the loading amount of DIAD, simplifies product isolation workup and increases the yields. The co-catalytic system proved to be effective not only with DIAD but also with the reduced forms of DIAD-H(2). Supported by predictions of in-house developed ligand-based target fishing computational tools, a series of newly synthesized 1-phenylethynyl-substituted THBN derivatives were evaluated against both human cholinesterases (AChE, BChE), and monoamine oxidases (MAOs A and B) as well. Most 1-phenylethynyl-substituted THBNs, and the respective 1-unsubstituted derivatives, inhibited AChE and BChE at single-digit micromolar concentrations (IC(50) = 3-10 microM). Molecular docking simulations suggested that the ethynyl substituent may engage additional hydrophobic/pi-pi interactions in the AChE binding sites. Notably, 12-chloro-1,2,3,4-tetrahydro-2-methylnaphtho[2,3-b][1,6]naphthyridine (3a) proved to be also a potent and selective MAO A inhibitor (IC(50) = 0.237 microM, selectivity index > 40). CI - (c) 2026 Deutsche Pharmazeutische Gesellschaft. FAU - Kulikova, Larisa N AU - Kulikova LN AD - Organic Chemistry Department, Peoples' Friendship University of Russia (RUDN University), Moscow, Russian Federation. FAU - Deruvo, Caterina AU - Deruvo C AD - Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Bari, Italy. FAU - Listratova, Anna V AU - Listratova AV AUID- ORCID: 0000-0003-0654-9451 AD - Organic Chemistry Department, Peoples' Friendship University of Russia (RUDN University), Moscow, Russian Federation. FAU - Reza, Raesi Gh AU - Reza RG AD - Organic Chemistry Department, Peoples' Friendship University of Russia (RUDN University), Moscow, Russian Federation. FAU - Iurina, Ekaterina S AU - Iurina ES AD - Organic Chemistry Department, Peoples' Friendship University of Russia (RUDN University), Moscow, Russian Federation. FAU - de Candia, Modesto AU - de Candia M AUID- ORCID: 0000-0002-4570-1981 AD - Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Bari, Italy. FAU - Gambacorta, Nicola AU - Gambacorta N AD - Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Bari, Italy. FAU - Nicolotti, Orazio AU - Nicolotti O AUID- ORCID: 0000-0001-6533-5539 AD - Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Bari, Italy. FAU - Voskressensky, Leonid G AU - Voskressensky LG AD - Organic Chemistry Department, Peoples' Friendship University of Russia (RUDN University), Moscow, Russian Federation. FAU - Altomare, Cosimo D AU - Altomare CD AUID- ORCID: 0000-0001-5016-5805 AD - Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Bari, Italy. LA - eng PT - Journal Article PL - Germany TA - Arch Pharm (Weinheim) JT - Archiv der Pharmazie JID - 0330167 RN - 0 (Cholinesterase Inhibitors) RN - 0 (Monoamine Oxidase Inhibitors) RN - EC 1.4.3.4 (Monoamine Oxidase) RN - 0 (Naphthyridines) RN - EC 3.1.1.7 (Acetylcholinesterase) RN - EC 3.1.1.8 (Butyrylcholinesterase) SB - IM MH - *Cholinesterase Inhibitors/chemical synthesis/pharmacology/chemistry MH - *Monoamine Oxidase Inhibitors/chemical synthesis/pharmacology/chemistry MH - Humans MH - Structure-Activity Relationship MH - Monoamine Oxidase/metabolism/drug effects MH - *Naphthyridines/chemical synthesis/pharmacology/chemistry MH - Molecular Docking Simulation MH - Molecular Structure MH - Acetylcholinesterase/metabolism MH - Butyrylcholinesterase/metabolism MH - Drug Design MH - Animals MH - Dose-Response Relationship, Drug OTO - NOTNLM OT - benzonaphthyridines OT - cholinesterase inhibitors OT - cross-coupling ethynylation OT - neurologic disorders EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 15:13 PHST- 2026/10/06 00:00 [revised] PHST- 2026/08/01 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 15:13 [entrez] AID - e70373 [pii] AID - 10.1002/ardp.70373 [doi] PST - ppublish SO - Arch Pharm (Weinheim). 2026 Nov;359(11):e70373. doi: 10.1002/ardp.70373. PMID- 42956271 OWN - NLM STAT- Publisher LR - 20261029 IS - 1537-453X (Electronic) IS - 0277-3732 (Linking) DP - 2026 Oct 20 TI - Efficacy and Safety of KRAS-Targeted Therapy in Locally Advanced or Metastatic Pancreatic Ductal Adenocarcinoma: An Updated Systematic Review and Meta-Analysis of Pooled Proportions. LID - 10.1097/COC.0000000000001387 [doi] AB - OBJECTIVES: Kirsten rat sarcoma viral oncogene homolog mutations drive over 90% of pancreatic ductal adenocarcinoma (PDAC). Direct RAS inhibitors have shown early activity, but the only prior meta-analysis pooled early-phase monotherapy cohorts and predates the first phase 3 randomized trial and newer combination regimens. We estimated the objective response rate (ORR), disease control rate (DCR), and safety of Kirsten rat sarcoma viral oncogene homolog-targeted therapy in locally advanced or metastatic PDAC. METHODS: PubMed/MEDLINE, Embase, and SCOPUS were searched from inception through July 2026. Single-arm proportions were pooled using a random-effects generalized linear mixed model; RASolute 302 was analyzed separately. Prespecified subgroups stratified ORR by mechanistic class and line of therapy. RESULTS: A total of 1008 patients from 16 cohorts across 7 trials were included; 15 single-arm cohorts (507 patients) contributed to pooled estimates. Pooled ORR was 38.9% (95% CI: 29.9%-48.8%; I2=75.7%) and varied significantly by mechanism (P<0.0001): combination regimens showed the highest activity (60.5%), followed by G12D-selective (31.0%), pan-RAS(ON) (26.6%), and G12C-selective monotherapy (25.4%). Response declined with successive lines of therapy (P=0.0102), from 72.5% in treatment-naive patients to 32.5% after >/=3 prior lines. Pooled DCR was 87.6% (95% CI: 82.8%-91.2%). Grade >/=3 treatment-related adverse events occurred in 47.3%. In RASolute 302, daraxonrasib nearly doubled median overall survival versus chemotherapy (13.2 vs. 6.6 mo). CONCLUSIONS: Kirsten rat sarcoma viral oncogene homolog-directed therapy produces meaningful antitumor activity in previously treated PDAC, greatest for combination regimens and earlier treatment; the evidence remains heterogeneous and largely early-phase, and randomized trials are needed before routine adoption. CI - Copyright (c) 2026 Wolters Kluwer Health, LLC. All rights reserved. FAU - Fatima, Faiza AU - Fatima F AD - Services Institute of Medical Sciences, Lahore, Pakistan. FAU - Javed, Muhammad A AU - Javed MA AD - King Edward Medical University, Lahore, Pakistan. FAU - Mirza, Aeliya AU - Mirza A AD - Lahore Medical and Dental College, Lahore, Pakistan. FAU - Ahmad, Shahzaib AU - Ahmad S AD - Texas Tech University Hospital Centre, Amarillo, TX. FAU - Shahzad, Zara AU - Shahzad Z AUID- ORCID: 0000-0003-3661-2300 AD - Lahore Medical and Dental College, Lahore, Pakistan. FAU - Murad, Ali AU - Murad A AD - Lahore Medical and Dental College, Lahore, Pakistan. LA - eng PT - Journal Article DEP - 20261020 PL - United States TA - Am J Clin Oncol JT - American journal of clinical oncology JID - 8207754 SB - IM OTO - NOTNLM OT - KRAS OT - RAS inhibitor OT - daraxonrasib OT - meta-analysis OT - pancreatic ductal adenocarcinoma OT - targeted therapy COIS- The authors declare no conflict of interest. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 15:13 PHST- 2026/09/01 00:00 [received] PHST- 2026/09/03 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 15:13 [entrez] AID - 00000421-990000000-00467 [pii] AID - 10.1097/COC.0000000000001387 [doi] PST - aheadofprint SO - Am J Clin Oncol. 2026 Oct 20. doi: 10.1097/COC.0000000000001387. PMID- 42956258 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1939-2222 (Electronic) IS - 0022-1015 (Linking) VI - 155 IP - 11 DP - 2026 Nov TI - How we see relations: The case of containment and support. PG - 2888-2902 LID - 10.1037/xge0002012 [doi] AB - To navigate and act in the environment, it is necessary to recognize objects and the relationships between them. Recognition of objects in visual scenes is rapid and automatic. Are relations between objects in a scene also extracted rapidly and automatically during perception? Here, we considered containment and support, two relations that are fundamental to our understanding of the physical world. Using frequency-tagging electroencephalography on human subjects, we measured stimulus-driven neural responses during rapid visual presentation of images (4 Hz), with a periodic oddball structure to isolate the selective response to object pairs presented side-by-side (e.g., a book and a table) versus two objects in support or containment relations (e.g., a book on a table). We found enhanced responses to objects in support/containment relations (vs. objects side-by-side) when a relation was instantiated by different exemplars of the same scene (e.g., a book on a table), but not by different scenes involving distinct object categories (e.g., a book on a table, a lamp on a stand, a laptop on a desk). These findings indicate that, during scene perception, relations are extracted in a format that generalizes within, but not across, types of visual scenes. We propose that, during perception, relations are not represented as discrete, abstract tokens ("in" or "on") but rather as objects' properties, such that changes in the objects entail changes in the relational representation. These results provide new insight into the perceptual foundations of relational processing underlying physical reasoning. (PsycInfo Database Record (c) 2026 APA, all rights reserved). FAU - Vettori, Sofie AU - Vettori S AUID- ORCID: 0000-0002-3190-514X AD - Institut des Sciences Cognitives Marc Jeannerod, Unite Mixte de Recherche 5229, Centre National de la Recherche Scientifique and Universite Claude Bernard Lyon 1. FAU - Rostami, Farzad AU - Rostami F AUID- ORCID: 0009-0007-5735-9873 AD - Institut des Sciences Cognitives Marc Jeannerod, Unite Mixte de Recherche 5229, Centre National de la Recherche Scientifique and Universite Claude Bernard Lyon 1. FAU - Hochmann, Jean-Remy AU - Hochmann JR AD - Institut des Sciences Cognitives Marc Jeannerod, Unite Mixte de Recherche 5229, Centre National de la Recherche Scientifique and Universite Claude Bernard Lyon 1. FAU - Papeo, Liuba AU - Papeo L AUID- ORCID: 0000-0003-3056-8679 AD - Institut des Sciences Cognitives Marc Jeannerod, Unite Mixte de Recherche 5229, Centre National de la Recherche Scientifique and Universite Claude Bernard Lyon 1. LA - eng GR - Labex Cortex of the University of Lyon/ GR - Fondation de France/ GR - Agence Nationale de la Recherche/ GR - Deutsche Forschungsgemeinschaft Franco-German/ PT - Journal Article PL - United States TA - J Exp Psychol Gen JT - Journal of experimental psychology. General JID - 7502587 SB - IM MH - Humans MH - *Pattern Recognition, Visual/physiology MH - Electroencephalography MH - Female MH - Male MH - Adult MH - Young Adult MH - *Visual Perception/physiology MH - *Recognition, Psychology/physiology EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 15:05 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 15:05 [entrez] AID - 2028-48315-007 [pii] AID - 10.1037/xge0002012 [doi] PST - ppublish SO - J Exp Psychol Gen. 2026 Nov;155(11):2888-2902. doi: 10.1037/xge0002012. PMID- 42956239 OWN - NLM STAT- Publisher LR - 20261029 IS - 1573-661X (Electronic) IS - 0147-7307 (Linking) DP - 2026 Oct 29 TI - Examining college sexual violence mandatory reporting policies and report outcomes: Evidence does not support a universal approach over alternative approaches. LID - 10.1037/lhb0000686 [doi] AB - OBJECTIVE: Mandatory reporting policies for sexual violence in U.S. institutions of higher education (IHEs) require certain designated employees to report any potential sexual violence they learn about to IHE officials, even when the victim/survivor does not want to report. The most common policy approach is a Universal approach, in which all or nearly all employees are designated as mandatory reporters. However, some have adopted Alternative approaches (e.g., designating only select employees as mandatory reporters, considering survivor consent to the report). It is often assumed that Universal policies best facilitate beneficial outcomes, such as addressing cases of sexual violence and supporting survivors. Yet, these assumptions have not been supported with empirical evidence. HYPOTHESES: This exploratory study examined if having a Universal approach (vs. an Alternative approach) was associated with the number of reports made to the Title IX office and key report outcomes following a mandated report. There were no formal hypotheses. METHOD: This study used survey data collected from a national sample of Title IX practitioners in IHEs (N = 260). RESULTS: Title IX practitioners working at IHEs with a Universal policy received significantly more reports from mandatory reporters than those with an Alternative policy. However, there were no clear significant differences between policy approaches for other outcomes, including total reports, percentage of reports possibly actionable under Title IX policy, how frequently survivors responded to contact from the Title IX office, or how frequently survivors wanted or received a formal investigation process, accommodations, or resource referrals. CONCLUSIONS: There were few differences between policy approaches for important mandatory report outcomes, refuting assumptions that Universal mandatory reporting policies are associated with better outcomes than Alternative approaches. (PsycInfo Database Record (c) 2026 APA, all rights reserved). FAU - Holland, Kathryn J AU - Holland KJ AUID- ORCID: 0000-0001-8340-4702 AD - Department of Psychology, University of Nebraska-Lincoln. FAU - Howard Valdivia, Rebecca L AU - Howard Valdivia RL AUID- ORCID: 0009-0004-7709-6167 AD - Department of Psychology, University of Nebraska-Lincoln. FAU - Goodman-Williams, Rachael AU - Goodman-Williams R AUID- ORCID: 0000-0002-9458-9703 AD - Department of Justice, Law and Criminology, American University. LA - eng GR - Center for Institutional Courage/ PT - Journal Article DEP - 20261029 PL - United States TA - Law Hum Behav JT - Law and human behavior JID - 7801255 SB - IM EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 15:04 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 15:04 [entrez] AID - 2028-47356-001 [pii] AID - 10.1037/lhb0000686 [doi] PST - aheadofprint SO - Law Hum Behav. 2026 Oct 29. doi: 10.1037/lhb0000686. PMID- 42956231 OWN - NLM STAT- Publisher LR - 20261029 IS - 1939-1277 (Electronic) IS - 0096-1523 (Linking) DP - 2026 Oct 29 TI - Dissociation between spontaneous gaze shifts and the spatial coding of number magnitude: Evidence from four high-resolution eye-tracking experiments. LID - 10.1037/xhp0001456 [doi] AB - Does the brain representation of number magnitude inherently involve space? Early studies suggested that merely perceiving or judging numbers automatically shifts attention and gaze leftward for small numbers and rightward for large ones, supporting the existence of an inherent space-number association. More recent evidence, however, has challenged this view, indicating that spatial-numerical effects arise primarily when spatial and magnitude codes are jointly engaged for response selection. Across four high-resolution eye-tracking experiments, we tested whether discriminating the magnitude of spoken number words elicits overt oculomotor gaze shifts. In Experiments 1-3, participants made verbal magnitude judgments to auditorily presented number words (1-9, excluding 5) while viewing a blank screen (Experiment 1), a Hermann grid designed to facilitate eye movements (Experiment 2), or horizontally symmetrical landscapes presented with or without lateral distractors (Experiment 3). Across all conditions, we found no evidence of horizontal or vertical gaze shifts associated with number magnitude. In Experiment 4, participants explicitly mapped magnitude judgments onto spatial response codes, producing a space-number association of response codes effect. Nevertheless, no spontaneous magnitude-congruent gaze shifts emerged. These findings indicate that number magnitude does not inherently trigger spatially directed eye movements and that spatial-numerical associations depend on task-driven recruitment of both magnitude and spatial response codes. (PsycInfo Database Record (c) 2026 APA, all rights reserved). FAU - Lo Presti, Sara AU - Lo Presti S AD - Department of Psychology, Sapienza" University of Rome. FAU - Mauti, Marika AU - Mauti M AD - Department of Psychology, Sapienza" University of Rome. FAU - Lozito, Silvana AU - Lozito S AD - Department of Psychology, Sapienza" University of Rome. FAU - Piga, Valentina AU - Piga V AD - Department of Psychology, Sapienza" University of Rome. FAU - Scuderi, Angelica AU - Scuderi A AD - Department of Psychology, Sapienza" University of Rome. FAU - Martelli, Marialuisa AU - Martelli M AD - Department of Psychology, Sapienza" University of Rome. FAU - Lasaponara, Stefano AU - Lasaponara S AD - Department of Psychology, Sapienza" University of Rome. FAU - Doricchi, Fabrizio AU - Doricchi F AUID- ORCID: 0000-0002-1535-9046 AD - Department of Psychology, Sapienza" University of Rome. LA - eng GR - Italian Ministry of University and Research/ GR - Sapienza University of Rome/ PT - Journal Article DEP - 20261029 PL - United States TA - J Exp Psychol Hum Percept Perform JT - Journal of experimental psychology. Human perception and performance JID - 7502589 SB - IM EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 15:04 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 15:04 [entrez] AID - 2028-48365-001 [pii] AID - 10.1037/xhp0001456 [doi] PST - aheadofprint SO - J Exp Psychol Hum Percept Perform. 2026 Oct 29. doi: 10.1037/xhp0001456. PMID- 42956229 OWN - NLM STAT- Publisher LR - 20261029 IS - 1939-2117 (Electronic) IS - 0022-006X (Linking) DP - 2026 Oct 29 TI - Evaluating large language models versus human therapists in generating interventions for patients with depression in psychodynamic psychotherapy. LID - 10.1037/ccp0001034 [doi] AB - OBJECTIVE: Recent advances in artificial intelligence, especially the rise of large language models (LLMs), hold great promise for transforming mental health care, but their growing integration also raises pressing questions about their ability to deliver meaningful therapeutic interventions. This study compared the capabilities of LLMs to those of human therapists in producing context-sensitive psychotherapy responses within 16-session supportive-expressive psychodynamic psychotherapy for patients with depression. Specifically, it compared LLM and human therapists on (a) the frequency and typicality of their intervention choice and (b) the quality and general helpfulness of their interventions. METHOD: We developed an LLM-based framework composed of four specialized LLM agents, each representing a different therapeutic dimension, and a Judge-LLM that selects the most appropriate intervention for a given context. A sample of 300 therapy session segments, each containing both human- and LLM-generated responses, served as the evaluation data set. Expert clinical judges assessed interventions for typicality and quality. RESULTS: LLMs and human therapists relied on broadly similar intervention types. However, the contextual choice of when to use a specific type differed. Human interventions were rated as generally more helpful. Specifically, humans produced higher quality meaning-making interventions, while no differences were found for supportive, directive, or exploratory interventions. CONCLUSIONS: The findings suggest that under the specific locally deployed multiagent configuration tested, LLMs can generate a range of context-sensitive therapeutic responses but showed limitations in producing high-quality meaning-making interventions. These findings highlight both the promise and the current boundaries of such systems as supervised therapeutic adjuncts. (PsycInfo Database Record (c) 2026 APA, all rights reserved). FAU - Bar-Shachar, Yael AU - Bar-Shachar Y AUID- ORCID: 0000-0003-0354-7493 AD - Department of Psychology, Bar-Ilan University. FAU - Rafael, Dana AU - Rafael D AUID- ORCID: 0009-0005-5764-7133 AD - Department of Psychology, Bar-Ilan University. FAU - Goel, Anmol AU - Goel A AUID- ORCID: 0009-0009-4807-2402 AD - Department of Computer Science, Hessian Center for Artificial Intelligence, Technical University of Darmstadt. FAU - Klein, Ayal AU - Klein A AUID- ORCID: 0000-0003-2665-7898 AD - Department of Computer Science, Ariel University. FAU - Gurevych, Iryna AU - Gurevych I AUID- ORCID: 0000-0003-2187-7621 AD - Department of Computer Science, Hessian Center for Artificial Intelligence, Technical University of Darmstadt. FAU - Atzil-Slonim, Dana AU - Atzil-Slonim D AUID- ORCID: 0000-0001-6958-1200 AD - Department of Psychology, Bar-Ilan University. LA - eng GR - Israel Science Foundation/ PT - Journal Article DEP - 20261029 PL - United States TA - J Consult Clin Psychol JT - Journal of consulting and clinical psychology JID - 0136553 SB - IM EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 15:03 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 15:03 [entrez] AID - 2028-47351-001 [pii] AID - 10.1037/ccp0001034 [doi] PST - aheadofprint SO - J Consult Clin Psychol. 2026 Oct 29. doi: 10.1037/ccp0001034. PMID- 42956223 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1935-990X (Electronic) IS - 0003-066X (Linking) VI - 81 IP - 8 DP - 2026 Nov TI - Taking a bottom-up, data-driven approach to characterizing variability across early-life environments. PG - 1191-1206 LID - 10.1037/amp0001738 [doi] AB - Children's long-term outcomes correlate with the physical and social features of the homes, schools, and neighborhoods in which they grow up. Yet, current techniques for establishing these correlations are limited. This article (a) presents five main issues linked to the currently used approaches. For example, most research relies on questionnaires or coding brief videoed interactions, which focus on a limited set of dimensions selected by researchers ("top-down" approach). As a result, these methods are limited because they provide only a static snapshot, which often conflates conceptually diverse levels, including the physical (e.g., noise, light) and social environments (e.g., caregiver mood, responsiveness). New technologies offer alternative approaches to these issues. This article (b) highlights how continuous multifeature observations, processed using machine learning tools, can improve our description of early development and inform theorizing of the mechanisms through which environments shape outcomes. Devices worn by children and those around them provide long-form recordings, allowing researchers to track how naturalistic environments vary over time and how children respond to them in real time. This shift supports a more dynamic, bottom-up, data-driven, and potentially culturally neutral approach to characterizing environmental variability. Finally, the article (c) discusses key limitations, notably the need to ensure that machine learning methods used to analyze early environments are trained using culturally diverse data, evaluating remaining risks of bias, and practical and ethical considerations. (PsycInfo Database Record (c) 2026 APA, all rights reserved). FAU - Aguilo-Mayans, Marian AU - Aguilo-Mayans M AUID- ORCID: 0009-0005-3203-7363 AD - Institute for the Science of Early Years and Youth, University of East London. FAU - Penaherrera-Velez, Maria Jose AU - Penaherrera-Velez MJ AD - Institute for the Science of Early Years and Youth, University of East London. FAU - Airaksinen, Manu AU - Airaksinen M AD - Department of Physiology, University of Helsinki. FAU - Cristia, Alejandrina AU - Cristia A AD - Laboratoire de Sciences Cognitives et Psycholinguistique, Departement d'Etudes Cognitives, Ecole Normale Superieure, French National Centre for Scientific Research, Paris Sciences et Lettres University. FAU - Leppanen, Jukka M AU - Leppanen JM AD - Department of Psychology, University of Turku. FAU - Warreyn, Petra AU - Warreyn P AD - Department of Experimental Clinical and Health Psychology, Ghent University. FAU - Marschik, Peter B AU - Marschik PB AD - Child and Adolescent Psychiatry and Psychotherapy, University Hospital Heidelberg, Heidelberg University. FAU - Labendzki, Pierre AU - Labendzki P AD - Institute for the Science of Early Years and Youth, University of East London. FAU - Esposito, Giovanni AU - Esposito G AD - Institute for the Science of Early Years and Youth, University of East London. FAU - Ehatisham-Ul-Haq, Muhammad AU - Ehatisham-Ul-Haq M AD - Institute for the Science of Early Years and Youth, University of East London. FAU - Virtanen, Tuomas AU - Virtanen T AD - Faculty of Information Technology and Communication Sciences, University of Tampere. FAU - Zhao, Guoying AU - Zhao G AD - European Laboratory for Learning and Intelligent Systems Institute Finland. FAU - Jessen, Sarah AU - Jessen S AD - Institute of Medical Psychology, University of Luebeck. FAU - Lockwood-Estrin, Georgia AU - Lockwood-Estrin G AD - Department of Psychology and Human Development, School of Childhood and Social Care, University of East London. FAU - Mukherjee, Debarati AU - Mukherjee D AD - Public Health Foundation of India-Centre for Developmental and Lifecourse Research, Public Health Foundation of India. FAU - Benga, Oana AU - Benga O AD - Department of Psychology, Babes-Bolyai University. FAU - Hens, Kristien AU - Hens K AD - Department of Philosophy, University of Antwerp. FAU - Van Heerden, Alastair AU - Van Heerden A AD - SYNAPSE Research Collective, Wits Health Consortium. FAU - Wass, Sam V AU - Wass SV AD - Institute for the Science of Early Years and Youth, University of East London. LA - eng PT - Journal Article PL - United States TA - Am Psychol JT - The American psychologist JID - 0370521 SB - IM MH - Humans MH - *Social Environment MH - *Child Development MH - *Machine Learning MH - Child MH - *Environment MH - Child, Preschool EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 15:03 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 15:03 [entrez] AID - 2028-47378-004 [pii] AID - 10.1037/amp0001738 [doi] PST - ppublish SO - Am Psychol. 2026 Nov;81(8):1191-1206. doi: 10.1037/amp0001738. PMID- 42956214 OWN - NLM STAT- Publisher LR - 20261029 IS - 1939-0599 (Electronic) IS - 0012-1649 (Linking) DP - 2026 Oct 29 TI - Association between daily parental surveillance and adolescent privacy and autonomy. LID - 10.1037/dev0002280 [doi] AB - Adolescence is marked by a tension between adolescents' privacy and autonomy development and parents' desire for knowledge about their adolescents' behaviors and whereabouts. While prior work has primarily examined parental monitoring behaviors that depend on control or solicitation of information, new parental surveillance technology enables parents to obtain objective information about their adolescent. However, the impacts of parental surveillance technology use on core adolescent developmental needs-like privacy and autonomy-are not well understood. This study (N = 142 parent-adolescent dyads and N = 3,588 survey days) used a 4-week daily diary design to examine the association between parents' daily use of parental monitoring behaviors and adolescents' daily experiences of privacy and autonomy. Parental surveillance technology use was associated with adolescents' feelings of privacy invasion the following day, but there was little evidence of an association between parents' surveillance technology use and adolescents' feelings of privacy invasion and autonomy on the same day. Results indicate that parental surveillance technology use may impact adolescents' feelings of privacy invasion but may not have broad negative impacts on other adolescent development needs, like autonomy, on a day-to-day basis. While parental surveillance technology is intended to support adolescents' physical safety, this work highlights the psychological impacts of its use and the ways it may hinder adolescent development on a daily level. (PsycInfo Database Record (c) 2026 APA, all rights reserved). FAU - Brinberg, Miriam AU - Brinberg M AUID- ORCID: 0000-0001-5202-9292 AD - School of Communication, Ohio State University. FAU - Gong, Yihui AU - Gong Y AUID- ORCID: 0000-0002-9686-5441 AD - School of Communication, Ohio State University. FAU - Pourafshari, Razieh AU - Pourafshari R AUID- ORCID: 0000-0002-3672-4874 AD - School of Communication, Ohio State University. FAU - Colaianne, Blake A AU - Colaianne BA AUID- ORCID: 0000-0001-5111-5527 AD - Department of Human Development and Family Studies, Pennsylvania State University. LA - eng GR - Mental Research Institute/ GR - National Institutes of Health; National Institute of Mental Health/ PT - Journal Article DEP - 20261029 PL - United States TA - Dev Psychol JT - Developmental psychology JID - 0260564 SB - IM EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 15:03 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 15:03 [entrez] AID - 2028-47719-001 [pii] AID - 10.1037/dev0002280 [doi] PST - aheadofprint SO - Dev Psychol. 2026 Oct 29. doi: 10.1037/dev0002280. PMID- 42956193 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2564-1891 (Electronic) IS - 2564-1891 (Linking) VI - 6 DP - 2026 Oct 29 TI - Online COVID-19 Misinformation and Infodemic Governance During the Peak Pandemic Period: Scoping Review of Social Media Research From 2020 to 2022. PG - e79434 LID - 10.2196/79434 [doi] AB - BACKGROUND: During the COVID-19 pandemic, social media platforms became major channels for public health communication and public sensemaking, but they also enabled the rapid circulation of rumors, false claims, conspiracy narratives, and misleading health information. Research on online COVID-19 misinformation expanded rapidly during the peak pandemic period, yet the evidence base was fragmented across public health, computing, communication, and information science. OBJECTIVE: This scoping review synthesizes empirical research on online COVID-19 misinformation on social media during the peak pandemic period from February 2020 to October 2022. It examines how misinformation was conceptualized, which platforms and public health contexts were emphasized, and how computational methods shaped the evidence base for infodemic management. Its distinct contribution is an integrated comparison of conceptual definitions, thematic and platform concentration, the computational research pipeline, and the gap between detection and actionable infodemic governance. METHODS: We conducted a PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses)-informed scoping review of empirical studies indexed in PubMed and IEEE Xplore in November 2022 using COVID-19, social media platforms, and misinformation-related terms. Eligible reports were English-language, peer-reviewed empirical studies published between February 2020 and October 2022 that analyzed social media data using computational, quantitative, or social media analytic methods; the language restriction applied to the publication, not to the underlying dataset. The final synthesis included 100 studies selected from 1688 records. RESULTS: Within the included corpus, the literature was concentrated around vaccine-related discourse, public health and social measures-related misinformation, and text-based data from X (X Corp), formerly known as Twitter. Studies commonly used misinformation detection, classification, sentiment analysis, topic modeling, and network analysis. Fewer studies examined diffusion pathways, source tracing, cross-platform movement, intervention effectiveness, or platform governance. The evidence base remains more developed for detecting and describing misinformation than for evaluating actionable responses to it. CONCLUSIONS: Peak-pandemic COVID-19 misinformation research produced important computational and empirical knowledge, but it remains detection-centered and unevenly connected to public health action. Future infodemiology research should link computational outputs to intervention design, risk communication, fact-checking workflows, platform moderation, multilingual and multimodal analysis, and the evaluation of infodemic governance strategies. CI - (c) Yang Liu, Zhiying Yue. Originally published in JMIR Infodemiology (https://infodemiology.jmir.org). FAU - Liu, Yang AU - Liu Y AUID- ORCID: 0000-0002-4307-9738 AD - Department of Computer Information Systems, School of Business, North Carolina Central University, 1801 Fayetteville St, Durham, NC, 27707, United States, 1 9195305276. FAU - Yue, Zhiying AU - Yue Z AUID- ORCID: 0000-0003-1921-1409 AD - Digital Wellness Lab, Boston Children's Hospital, Harvard Medical School, Boston, MA, United States. LA - eng PT - Journal Article PT - Review PT - Scoping Review DEP - 20261029 PL - Canada TA - JMIR Infodemiology JT - JMIR infodemiology JID - 9918249014806676 SB - IM MH - *COVID-19/epidemiology MH - *Social Media/standards/statistics & numerical data MH - Humans MH - Pandemics MH - *Communication MH - *Infodemic MH - Public Health MH - SARS-CoV-2 MH - Digital Media OTO - NOTNLM OT - COVID-19 OT - infodemic management OT - infodemiology OT - machine learning OT - misinformation OT - natural language processing OT - public health communication OT - scoping review OT - social media EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 14:52 PHST- 2026/06/17 00:00 [received] PHST- 2026/09/02 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 14:52 [entrez] AID - v6i1e79434 [pii] AID - 10.2196/79434 [doi] PST - epublish SO - JMIR Infodemiology. 2026 Oct 29;6:e79434. doi: 10.2196/79434. PMID- 42956174 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2057-4347 (Electronic) IS - 2057-4347 (Linking) VI - 12 IP - 5 DP - 2026 Oct TI - Single-Nucleotide Polymorphisms in Calcium and Vitamin D Maintenance Genes Association With Tooth Size. LID - e70472 [pii] LID - 10.1002/cre2.70472 [doi] AB - OBJECTIVES: This study investigated the association between Single Nucleotide Polymorphisms (SNPs) in calcium and vitamin D maintenance genes and mesiodistal tooth size (MTS) of permanent teeth. MATERIALS AND METHODS: A total of 108 patients undergoing orthodontic treatment were recruited. Pretreatment orthodontic diagnostic casts were used to obtain the MTS. One single calibrated evaluator assessed the maximum crown dimensions in the mesiodistal direction of mandibular and maxillary permanent teeth with a digital caliper. Salivary genomic DNA was isolated from buccal cells, and six SNPs of the VDR, CYP24A1, CYP27B1, and PTH genes were selected. Real-Time PCR was performed for the genotyping analysis. For statistics analysis, uni- and multivariate tests accounting for multiple observations per-subject and adjusted by gender were performed (alpha = 5%). RESULTS: In multivariate analysis, the rs694 and rs6256 (PTH) SNPs were associated (p < 0.05) with smaller MTS of the maxillary first molar. The rs7975232 (VDR) and rs307247 (PTH) were associated with a smaller mesiodistal size of the mandibular first molar. The rs7975232 (VDR) was also associated with a smaller mesiodistal size of the maxillary central incisor and a greater mesiodistal size of the maxillary first premolar. The rs4646536 (CYP27B1) was also associated with greater mesiodistal size of the first premolar. CONCLUSION: SNPs in the PTH and vitamin D-related genes (VDR and CYP27B1) might be related to MTS variations of permanent teeth. CI - (c) 2026 The Author(s). Clinical and Experimental Dental Research published by John Wiley & Sons Ltd. FAU - Rodrigues, Lucas Cabral AU - Rodrigues LC AD - School of Dentistry, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil. FAU - Matsumoto, Mirian Aiko Nakane AU - Matsumoto MAN AD - Department of Pediatric Dentistry, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil. FAU - Stuani, Maria Bernadete Sasso AU - Stuani MBS AUID- ORCID: 0000-0001-7791-9144 AD - Department of Pediatric Dentistry, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil. FAU - Maranon-Vasquez, Guido Artemio AU - Maranon-Vasquez GA AD - Department of Pediatric Dentistry, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil. FAU - Romano, Fabio Lourenco AU - Romano FL AUID- ORCID: 0000-0003-1419-3520 AD - Department of Pediatric Dentistry, School of Dentistry of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, Sao Paulo, Brazil. FAU - de Oliveira, Daniela Silva Barroso AU - de Oliveira DSB AUID- ORCID: 0000-0003-2691-1411 AD - School of Dentistry, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil. FAU - Silva, Juliane Cora da AU - Silva JCD AD - School of Dentistry, Tuiuti University of Parana, Curitiba, Parana, Brazil. FAU - Baratto-Filho, Flares AU - Baratto-Filho F AUID- ORCID: 0000-0002-5649-7234 AD - School of Dentistry, Tuiuti University of Parana, Curitiba, Parana, Brazil. FAU - Kirschneck, Christian AU - Kirschneck C AUID- ORCID: 0000-0001-9473-8724 AD - Department of Orthodontics, University Hospital Bonn, Medical Faculty, Bonn, Nordrhein-Westfalen, Germany. FAU - Kuchler, Erika Calvano AU - Kuchler EC AUID- ORCID: 0000-0001-5351-2526 AD - Department of Orthodontics, University Hospital Bonn, Medical Faculty, Bonn, Nordrhein-Westfalen, Germany. FAU - Reis, Caio Luiz Bitencourt AU - Reis CLB AUID- ORCID: 0000-0003-0606-1632 AD - School of Dentistry, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil. LA - eng GR - 001/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/ GR - Kuchler/Kirschneck 2019/Alexander von Humboldt-Stiftung/ GR - 2021/02704-1/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/ PT - Journal Article PL - United States TA - Clin Exp Dent Res JT - Clinical and experimental dental research JID - 101692332 RN - 0 (Receptors, Calcitriol) RN - EC 1.14.15.16 (Vitamin D3 24-Hydroxylase) RN - EC 1.14.15.18 (25-Hydroxyvitamin D3 1-alpha-Hydroxylase) RN - EC 1.14.15.18 (CYP27B1 protein, human) RN - 0 (Parathyroid Hormone) RN - EC 1.14.15.16 (CYP24A1 protein, human) RN - 0 (VDR protein, human) RN - SY7Q814VUP (Calcium) RN - 1406-16-2 (Vitamin D) SB - IM MH - Humans MH - *Polymorphism, Single Nucleotide MH - Female MH - Male MH - *Receptors, Calcitriol/genetics MH - *Vitamin D3 24-Hydroxylase/genetics MH - *25-Hydroxyvitamin D3 1-alpha-Hydroxylase/genetics MH - *Parathyroid Hormone/genetics MH - Adolescent MH - *Calcium/metabolism MH - Genotype MH - *Tooth/anatomy & histology MH - Young Adult MH - Odontometry MH - Vitamin D/metabolism PMC - PMC13719053 OTO - NOTNLM OT - calcium OT - genetic OT - orthodontic OT - pth OT - tooth size OT - vitamin D COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 PMCR- 2026/10/29 CRDT- 2026/10/29 14:22 PHST- 2026/09/11 00:00 [revised] PHST- 2026/03/04 00:00 [received] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 14:22 [entrez] PHST- 2026/10/29 00:00 [pmc-release] AID - e70472 [pii] AID - CRE270472 [pii] AID - 10.1002/cre2.70472 [doi] PST - ppublish SO - Clin Exp Dent Res. 2026 Oct;12(5):e70472. doi: 10.1002/cre2.70472. PMID- 42956164 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2057-4347 (Electronic) IS - 2057-4347 (Linking) VI - 12 IP - 5 DP - 2026 Oct TI - Evaluation of Positional Variability in Surface Roughness in Orthodontic Mini-Implants Using Confocal Microscopy: A Multi-Manufacturer Comparative Study. LID - e70497 [pii] LID - 10.1002/cre2.70497 [doi] AB - OBJECTIVE: To compare the surface roughness of orthodontic mini-implants (OMIs) from seven manufacturers at standardized positions, since positional variability in OMI topography has not been systematically investigated but may influence healing and stability. MATERIALS AND METHODS: Seven OMI systems (n = 5 per system, total n = 35) were analyzed using 3D confocal laser scanning microscopy. Four locations per OMI were assessed (apex, mid, top, neck). Roughness parameters included Sa and Sz (microm), Sdr (%), Str (dimensionless), and Spc (1/mm). Linear mixed-effects models were fitted for each parameter with Manufacturer and Location as fixed effects and Implant ID as a random intercept. Fixed effects were evaluated by Type III ANOVA; estimated marginal means (EMMs, 95% CIs) and Tukey-adjusted pairwise contrasts summarized differences. RESULTS: Manufacturer, location, and their interaction were significant for all parameters (p < 0.0001). Dentaurum consistently showed the highest mean roughness values, especially at the neck, with an overall average value for Sa approximately 0.81 microm compared to 0.20-0.39 microm for others, Sz approximately 9.5 microm (others 2-3 microm), and Sdr approximately 6%-7% (others M2 coupling peaking at a short latency, as expected for a potentially direct transmission pathway. This transient interaction conveyed stimulus-selective information such that the S1 --> M2 communication subspace aligned with stimulus-coding axes in both regions. In contrast, M2 --> S1 feedback interactions were weaker and delayed. Brief optogenetic suppression of S1 reduced both M2 stimulus selectivity and behavioral discriminability, consistent with a functional role of the transient S1 --> M2 coupling. These findings demonstrate that sensorimotor transformation is supported not by sustained linear coupling along the stimulus- or choice- axis, but by a short-lived and highly selective feedforward communication channel that rapidly transmits reliable sensory representations into motor-planning circuits. CI - Copyright: (c) 2026 Ranjbar-Slamloo, Kamigaki. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Ranjbar-Slamloo, Yadollah AU - Ranjbar-Slamloo Y AUID- ORCID: 0000-0003-0831-3450 AD - Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, Singapore. FAU - Kamigaki, Tsukasa AU - Kamigaki T AD - Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, Singapore. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - PLoS Biol JT - PLoS biology JID - 101183755 SB - IM COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 14:02 PHST- 2026/01/07 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 14:02 [entrez] AID - PBIOLOGY-D-26-00037 [pii] AID - 10.1371/journal.pbio.3004095 [doi] PST - aheadofprint SO - PLoS Biol. 2026 Oct 29;24(10):e3004095. doi: 10.1371/journal.pbio.3004095. PMID- 42956068 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Evaluation of digital binocular therapies for amblyopia after refractive adaptation in treatment-naive children aged 3-13 years: A systematic review and meta-analysis of randomised controlled trials. PG - e0361102 LID - 10.1371/journal.pone.0361102 [doi] AB - BACKGROUND: Patching has been the mainstay of unilateral amblyopia treatment, but adherence is challenging. Digital binocular therapies have emerged as a novel approach; however, evidence in treatment-naive children remains limited, as existing studies have predominantly included previously treated children. OBJECTIVE: To evaluate the effectiveness of digital binocular therapies versus patching for improving visual acuity in treatment-naive children aged 3-13 years with unilateral amblyopia following refractive adaptation. DESIGN: A systematic review and meta-analysis of randomised controlled trials was conducted in accordance with PRISMA 2020 guidelines and registered in PROSPERO (CRD420261278100). Comprehensive literature searches were conducted from January 2000 to December 2025. Two independent reviewers screened and selected studies, extracted data, and assessed risk of bias using the Cochrane RoB 2 tool. The primary outcome was change in best-corrected visual acuity. Data were pooled using a random-effects model. The GRADE approach was used to rate the certainty of evidence. RESULTS: Five RCTs (n = 225) were included in the primary analysis. Digital binocular therapies showed no statistically significant difference in visual acuity compared with patching using a random-effects model (MD 0.00 logMAR, 95% CI -0.09 to 0.10; I2 = 50.2%). Sensitivity analysis including two additional RCTs judged to be at high risk of bias, one of which compared digital binocular therapy plus spectacles with spectacles alone (7 RCTs; n = 292), yielded similar results (MD 0.04 logMAR, 95% CI -0.03 to 0.10; I2 = 75.2%). The certainty of evidence for visual acuity was moderate, downgraded for inconsistency. CONCLUSION: Digital binocular therapies may serve as adjunctive treatment options when adherence to patching is challenging. However, current evidence does not demonstrate superiority over patching in treatment-naive children with unilateral amblyopia. The evidence remains limited by the small number of RCTs and modest sample sizes. REGISTRATION: This systematic review and meta-analysis was registered in the International Prospective Register of Systematic Reviews (PROSPERO) under registration number CRD420261278100 on 4 January 2026. CI - Copyright: (c) 2026 Narayanan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Narayanan, Anju Thadathikunnal AU - Narayanan AT AUID- ORCID: 0009-0002-8728-196X AD - Optometry Department, District Hospital Kanhangad, Kanhangad, Kerala, India. FAU - Robinson, David Grant AU - Robinson DG AUID- ORCID: 0009-0007-1320-559X AD - School of Optometry and Vision Sciences, Cardiff University, Cardiff, United Kingdom. FAU - Woodhouse, J Margaret AU - Woodhouse JM AD - School of Optometry and Vision Sciences, Cardiff University, Cardiff, United Kingdom. LA - eng PT - Journal Article PT - Meta-Analysis PT - Systematic Review DEP - 20261029 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Humans MH - *Amblyopia/therapy/physiopathology MH - Randomized Controlled Trials as Topic MH - Child MH - Child, Preschool MH - Visual Acuity/physiology MH - Adolescent MH - *Vision, Binocular/physiology MH - Treatment Outcome COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:53 PHST- 2026/08/21 00:00 [received] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:53 [entrez] AID - PONE-D-26-37622 [pii] AID - 10.1371/journal.pone.0361102 [doi] PST - epublish SO - PLoS One. 2026 Oct 29;21(10):e0361102. doi: 10.1371/journal.pone.0361102. eCollection 2026. PMID- 42956061 OWN - NLM STAT- Publisher LR - 20261029 IS - 1369-1627 (Electronic) IS - 0954-0261 (Linking) DP - 2026 Oct 29 TI - Continuous traumatic stress in Gaza: when caregivers and communities share the same crisis. PG - 1-5 LID - 10.1080/09540261.2026.2755000 [doi] AB - In Gaza, traumatic events do not belong only to the past. Repeated attacks, displacement, bereavement and deprivation continue to shape daily life, including the work of those providing healthcare. Drawing on my experience as a doctor living and working in Gaza during 2023-2025 and on selected clinical and humanitarian literature, I examine the usefulness of continuous traumatic stress (CTS) as a framework for understanding distress under ongoing threat. CTS does not replace post-traumatic stress disorder (PTSD) or other diagnoses. Rather, it draws attention to the difficulty of assessing fear, vigilance and impairment when danger is credible and protection is limited. I consider the effects on children and on healthcare workers who share their patients' circumstances, and discuss implications for mental health and psychosocial support (MHPSS). Clinical services remain essential, but their effectiveness depends partly on access to safety, food, shelter, medicines and functioning systems of care. FAU - Abu Mughaiseeb, Mohammed A AU - Abu Mughaiseeb MA AD - Medical advisor, Medecins Sans Frontieres (MSF-UK/IE), Ireland, UK. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Int Rev Psychiatry JT - International review of psychiatry (Abingdon, England) JID - 8918131 SB - IM OTO - NOTNLM OT - Continuous traumatic stress OT - Gaza OT - Healthcare workers OT - Protracted conflict OT - mental health and psychosocial support EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 13:53 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:53 [entrez] AID - 10.1080/09540261.2026.2755000 [doi] PST - aheadofprint SO - Int Rev Psychiatry. 2026 Oct 29:1-5. doi: 10.1080/09540261.2026.2755000. PMID- 42956049 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Improved model predictive current control strategy for overvoltage suppression in long-cable PWM-driven induction motor electrical submersible pump systems. PG - e0360296 LID - 10.1371/journal.pone.0360296 [doi] AB - Long-cable PWM-driven induction motor electric submersible pump (ESP) systems suffer from significant voltage reflection issues caused by distributed parameter effects, leading to transient overvoltages at the motor terminals that can reach several times the DC bus voltage. This severely threatens system stability and motor insulation integrity, and traditional control methods prove inadequate for effective suppression. To address this challenge, the paper proposes an improved model predictive current control (MPCC) strategy for induction motor ESP systems, which integrates overvoltage suppression and equivalent switching frequency reduction for long-cable PWM-driven applications. The reflection overvoltage mechanism is thoroughly analyzed through mathematical modeling of the motor and long-cable transmission line, and the applicability of predictive current control in such systems is investigated. The core improvement lies in a circular boundary constrained MPCC combined with a dynamic transition voltage vector selection mechanism, which enables dual-mode optimization based on current error boundary conditions: the normal cost function is applied within the circular boundary to jointly optimize voltage tracking, switching frequency, and dv/dt; while the backup cost function is activated outside the boundary to prioritize current error minimization. This effectively optimizes the voltage switching strategy, reducing both switching frequency and dv/dt, thereby preventing overvoltage excitation caused by abrupt voltage vector steps in PWM-driven systems. Simulation models under different cable configurations (1 km with RLC filter and 110 m without RLC filter) and an experimental platform based on a neutral-point-clamped three-level inverter (NPC-TLI) topology are established, with the 110 m no-filter configuration serving as a direct cross-validation baseline. Comparative simulation and experimental results demonstrate that the proposed strategy significantly reduces the duration of motor terminal overvoltage while maintaining excellent system dynamic performance. Moreover, the improved MPCC effectively suppresses overvoltage and reduces equivalent switching frequency without requiring hardware filter redesign, while the RLC filter remains necessary for full insulation safety compliance under more demanding long-cable conditions, substantially mitigating the risk of motor insulation breakdown. CI - Copyright: (c) 2026 Gong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Gong, Xiaopeng AU - Gong X AUID- ORCID: 0009-0003-0614-4216 AD - School of Mechanical Engineering, University of Science and Technology Beijing, Beijing, China. FAU - Qi, Xin AU - Qi X AUID- ORCID: 0000-0001-9130-8542 AD - School of Mechanical Engineering, University of Science and Technology Beijing, Beijing, China. FAU - Feng, Ming AU - Feng M AD - School of Mechanical Engineering, University of Science and Technology Beijing, Beijing, China. FAU - Zhu, Liancheng AU - Zhu L AUID- ORCID: 0000-0002-9984-390X AD - School of Electrical Engineering, Liaoning University of Technology, Jinzhou, China. FAU - Wang, Nianxing AU - Wang N AUID- ORCID: 0009-0003-3142-1625 AD - Daqing Oilfield Powerlift Industrial Pump Co. Ltd, Daqing, China. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - *Models, Theoretical MH - *Electricity MH - Computer Simulation COIS- And analysis, decision to publish, or preparation of the manuscript. The funder provided support in the form of salaries for author [NX W], but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the 'author contributions' section. This does not alter our adherence to PLOS ONE policies on sharing data and materials. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:52 PHST- 2026/06/11 00:00 [received] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:52 [entrez] AID - PONE-D-26-29296 [pii] AID - 10.1371/journal.pone.0360296 [doi] PST - epublish SO - PLoS One. 2026 Oct 29;21(10):e0360296. doi: 10.1371/journal.pone.0360296. eCollection 2026. PMID- 42956048 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Integrative genomic SEM analysis of shared genetic architecture and candidate genes for insulin resistance-related metabolic traits. PG - e0361601 LID - 10.1371/journal.pone.0361601 [doi] LID - e0361601 AB - Insulin resistance is a complex metabolic state involving impaired insulin sensitivity, compensatory insulin secretion, abnormal lipid metabolism, and altered fat distribution. However, the shared genetic architecture underlying these correlated phenotypes remains incompletely defined. In this study, we applied Genomic Structural Equation Modeling to genome-wide association summary statistics of fasting insulin, insulin sensitivity index, triglycerides, and waist-to-hip ratio to construct an insulin resistance-related latent genetic factor. SNP-level association analysis was then performed for the latent factor, followed by risk locus annotation, fine-mapping, transcriptome-wide association analysis, gene-level prioritization, enrichment analysis, and exploratory structural biology assessment of selected missense substitutions. The single-factor model showed acceptable but not optimal approximate fit and captured a biologically consistent genetic pattern, with positive loadings for fasting insulin, triglycerides, and waist-to-hip ratio and a negative loading for insulin sensitivity index. The latent factor showed detectable SNP heritability and revealed 3,067 genome-wide significant candidate SNPs, with Q_SNP retained as a complementary SNP-level heterogeneity diagnostic.. Functional annotation identified 100 genomic risk loci and 1,530 FDR-significant genes in MAGMA. Fine-mapping prioritized multiple candidate regions, including loci near PPARG, RSPO3, SLC38A11, SLC39A8, BCL2, GDF5, and SPATA5. TWAS and FOCUS further highlighted candidate genes involved in adipocyte biology, glucose-lipid metabolism, insulin secretion, and expression regulation, including PPARG, MLXIPL, TCF7L2, FAM13A, CCDC92, and SLC22A3. Enrichment analyses suggested that genetic signals associated with the insulin resistance-related latent factor were mainly linked to fat distribution, cardiometabolic traits, adipose tissue, mesenchymal cell lineages, and active regulatory genomic regions. In a separate exploratory analysis, AlphaFold3 modeling, ThermoMPNN prediction, and molecular dynamics simulations suggested structural perturbations for three ultra-rare missense substitutions in nominated candidate proteins: BCL2-W188G, GDF5-C400Y, and RSPO3-C129Y. These substitutions were selected using functional-impact annotations and modeling feasibility, were not GWAS lead or fine-mapped causal variants, and do not establish the mechanisms underlying the GWAS associations. These findings provide an integrated genetic and structural framework for understanding shared mechanisms underlying insulin resistance-related metabolic abnormalities, while emphasizing the need for further experimental validation. CI - Copyright: (c) 2026 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Li, ShuYan AU - Li S AD - Faculty of Chinese Medicine, Macau University of Science and Technology, Macau, China. AD - Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China. AD - Key Laboratory of Sichuan Province Ophthalmopathy Prevention & Cure and Visual Function Protection With TCM, Chengdu, China. FAU - Liang, Yi AU - Liang Y AD - Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China. FAU - Zhu, Guangwen AU - Zhu G AD - Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China. FAU - Wu, Lijiao AU - Wu L AD - Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China. FAU - Li, Hong AU - Li H AD - The First Affiliated Hospital of Chongqing University of Chinese Medicine, Chongqing, China. FAU - Li, Yu AU - Li Y AD - Faculty of Chinese Medicine, Macau University of Science and Technology, Macau, China. FAU - Chen, Qiu AU - Chen Q AUID- ORCID: 0000-0003-1089-4194 AD - Faculty of Chinese Medicine, Macau University of Science and Technology, Macau, China. AD - Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu, China. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (Insulin) RN - 0 (Triglycerides) SB - IM MH - *Insulin Resistance/genetics MH - Polymorphism, Single Nucleotide MH - Genome-Wide Association Study MH - Humans MH - Insulin/metabolism/blood MH - *Genomics/methods MH - Waist-Hip Ratio MH - Triglycerides/blood MH - Quantitative Trait Loci MH - Phenotype PMC - PMC13719024 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 PMCR- 2026/10/29 CRDT- 2026/10/29 13:52 PHST- 2026/06/28 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:52 [entrez] PHST- 2026/10/29 00:00 [pmc-release] AID - PONE-D-26-32325 [pii] AID - 10.1371/journal.pone.0361601 [doi] PST - epublish SO - PLoS One. 2026 Oct 29;21(10):e0361601. doi: 10.1371/journal.pone.0361601. eCollection 2026. PMID- 42956043 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1929-0748 (Electronic) IS - 1929-0748 (Linking) VI - 15 DP - 2026 Oct 29 TI - Development of CaReScreen, a Digital Health Tool for Assessing Late Effects and Cancer Rehabilitation Needs in Cancer Survivors: Protocol for a Qualitative Development and Formative Design Study. PG - e105443 LID - 10.2196/105443 [doi] AB - BACKGROUND: Due to advances in cancer diagnostics and treatments, life expectancy after cancer and the number of cancer survivors are increasing. Symptoms and late effects frequently occur in cancer survivors, with a negative impact on functioning and participation in society. Digital health tools might be helpful to systematically assess symptom burden of late effects and rehabilitation needs. OBJECTIVE: This paper describes the objectives, design, and current status of the Cancer Rehabilitation Screening (CaReScreen) project. The aims are (1) to report the completed development phase of the digital health tool CaReScreen, comprising the exploratory work, the establishment of the interdisciplinary team, the selection of the assessment instrument, and the development of the initial prototype, and (2) to define the objectives, design, and timelines of the ongoing validation of the scoring manual and of the planned large-scale feasibility study. METHODS: Development followed an iterative, qualitative, and participatory design approach guided by established frameworks for digital health development. It was informed by exploratory qualitative work comprising 40 interviews (9 group and 31 individual) involving 72 informants across Norway, which identified systematic assessment of late effects and navigation of rehabilitation services as the primary unmet needs in cancer survivorship follow-up. A 105-item patient-reported outcome measure (European Organization for Research and Treatment of Cancer [EORTC] QLQ-SURV100, supplemented with 5 EORTC QLQ-C30 items) was selected as the main assessment instrument. This paper reports the completed development phase; the ongoing validation study and the planned feasibility study are described in terms of objectives, design, and timelines, but their results are not reported here. RESULTS: An initial prototype was developed with health care professionals and cancer survivors as intended end users. It consists of a digital report that identifies and graphically visualizes a panoramic view of multidimensional needs, based on a traffic light scoring system: green indicates low symptom burden and high functioning, yellow moderate symptom burden and moderate functioning, and red high symptom burden and low functioning. The project is funded for the period 2022 to 2028. The development phase is completed, as well as early prototype usability and usefulness testing. Validation of the scoring manual is ongoing, with 800 participants included to date and reporting expected in spring 2027. A large-scale feasibility study is planned to commence in 2027. CONCLUSIONS: The CaReScreen interdisciplinary team has developed an initial digital health tool prototype with the main aim of enabling systematic assessment of late effects, functioning, and unmet rehabilitation needs in cancer survivors. The prototype now proceeds to validation and feasibility testing, which will determine its clinical utility and support regulatory approval under the European Medical Device Regulation. CI - (c) Maja Kulosman, Harald Kare Engan, Unn Merete Fagerli, Kristin Valborg Reinertsen, Kari Sand, Jon Arne Sandmael, Arne Solberg, Torgrim Tandstad. Originally published in JMIR Research Protocols (https://www.researchprotocols.org). FAU - Kulosman, Maja AU - Kulosman M AUID- ORCID: 0009-0004-2233-667X AD - Department of Clinical and Molecular Medicine, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology, P.O. 8905, Trondheim, Trondelag, 7491, Norway, 47 72853114. AD - Cancer Clinic, St Olav's University Hospital, Trondheim, Trondelag, Norway. FAU - Engan, Harald Kare AU - Engan HK AUID- ORCID: 0000-0002-4393-460X AD - Unicare, Oslo, Norway. FAU - Fagerli, Unn Merete AU - Fagerli UM AUID- ORCID: 0009-0007-3360-6542 AD - Department of Clinical and Molecular Medicine, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology, P.O. 8905, Trondheim, Trondelag, 7491, Norway, 47 72853114. AD - Cancer Clinic, St Olav's University Hospital, Trondheim, Trondelag, Norway. FAU - Reinertsen, Kristin Valborg AU - Reinertsen KV AUID- ORCID: 0000-0003-0433-8589 AD - Department of Oncology, Division of Cancer Medicine, Oslo University Hospital, Oslo, Norway. FAU - Sand, Kari AU - Sand K AUID- ORCID: 0000-0002-8391-2990 AD - Department of Health Services Research, SINTEF Digital, Trondheim, Trondelag, Norway. FAU - Sandmael, Jon Arne AU - Sandmael JA AUID- ORCID: 0000-0002-7944-4338 AD - Unicare, Oslo, Norway. AD - Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology, Trondheim, Trondelag, Norway. FAU - Solberg, Arne AU - Solberg A AUID- ORCID: 0000-0003-4461-6352 AD - Department of Clinical and Molecular Medicine, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology, P.O. 8905, Trondheim, Trondelag, 7491, Norway, 47 72853114. AD - Cancer Clinic, St Olav's University Hospital, Trondheim, Trondelag, Norway. FAU - Tandstad, Torgrim AU - Tandstad T AUID- ORCID: 0000-0002-9786-2138 AD - Department of Clinical and Molecular Medicine, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology, P.O. 8905, Trondheim, Trondelag, 7491, Norway, 47 72853114. AD - Cancer Clinic, St Olav's University Hospital, Trondheim, Trondelag, Norway. LA - eng PT - Journal Article DEP - 20261029 PL - Canada TA - JMIR Res Protoc JT - JMIR research protocols JID - 101599504 SB - IM MH - Humans MH - *Cancer Survivors/statistics & numerical data/psychology MH - Digital Health MH - Qualitative Research MH - Norway MH - *Neoplasms/rehabilitation MH - Feasibility Studies MH - Research Design MH - Patient Reported Outcome Measures OTO - NOTNLM OT - cancer rehabilitation OT - cancer survivors OT - decision support OT - digital development OT - digital health tools OT - eHealth OT - late effects OT - need assessment EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:44 PHST- 2026/07/02 00:00 [received] PHST- 2026/09/04 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:44 [entrez] AID - v15i1e105443 [pii] AID - 10.2196/105443 [doi] PST - epublish SO - JMIR Res Protoc. 2026 Oct 29;15:e105443. doi: 10.2196/105443. PMID- 42956033 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1091-6490 (Electronic) IS - 0027-8424 (Linking) VI - 123 IP - 44 DP - 2026 Nov 3 TI - Elucidating molecular regulatory rules of nanoparticle biological identity via standardized deep proteomics. PG - e2618716123 LID - 10.1073/pnas.2618716123 [doi] AB - The biological identity of nanoparticles is often redefined upon exposure to physiological fluids through the formation of a protein corona (PC), However, the molecular rules linking synthetic design to biological fate remain elusive owing to the fragmented and inconsistent proteomics data. Here, leveraging a standardized deep proteomic database of 42 diverse nanomaterials, we reveal the general molecular selection rules that govern the PC formation. We demonstrate that the PC is not a product of random adsorption but is shaped by strict property-dependent recruitment. First, surface charge regulates cellular uptake, with anionic nanoparticles specifically recruiting adhesion proteins to promote Itgav-mediated endocytosis, thus forming a causal charge-corona-receptor chain. Second, PEGylation engineers immune evasion by selectively depleting specific complement and coagulation factors, rather than merely preventing protein adsorption. Third, nanoparticle geometry imposes structural constraints and selects proteins based on specific physicochemical features such as lysine content. Beyond mechanistic insights, we show that material-specific PCs can rebalance the serum proteome and enable the detection of low-abundance disease biomarkers. This work shifts the paradigm from empirical description toward rule-based prediction and provides a foundational framework for rationally engineering nanomedicines with predictable and programmable biological identities. FAU - Jiang, Chao AU - Jiang C AUID- ORCID: 0009-0002-0441-8909 AD - Zhejiang Cancer Hospital, Zhejiang Provincial Key Laboratory of Protein Detection Technology and Diagnostic Equipment, Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou 310022, Zhejiang, People's Republic of China. ROR: https://ror.org/04vs9wp72 AD - Cancer Center and Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Macau 999078, China. FAU - Huang, Haozhe AU - Huang H AD - Zhejiang Cancer Hospital, Zhejiang Provincial Key Laboratory of Protein Detection Technology and Diagnostic Equipment, Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou 310022, Zhejiang, People's Republic of China. ROR: https://ror.org/04vs9wp72 FAU - Gao, Rongzi AU - Gao R AD - Zhejiang Cancer Hospital, Zhejiang Provincial Key Laboratory of Protein Detection Technology and Diagnostic Equipment, Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou 310022, Zhejiang, People's Republic of China. ROR: https://ror.org/04vs9wp72 AD - School of Life Sciences, Tianjin University, Tianjin 300072, China. ROR: https://ror.org/012tb2g32 FAU - Du, Yuling AU - Du Y AD - Zhejiang Cancer Hospital, Zhejiang Provincial Key Laboratory of Protein Detection Technology and Diagnostic Equipment, Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou 310022, Zhejiang, People's Republic of China. ROR: https://ror.org/04vs9wp72 AD - School of Molecular Medicine, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, Zhejiang, China. ROR: https://ror.org/05qbk4x57 FAU - Jin, Baichuan AU - Jin B AD - Zhejiang Cancer Hospital, Zhejiang Provincial Key Laboratory of Protein Detection Technology and Diagnostic Equipment, Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou 310022, Zhejiang, People's Republic of China. ROR: https://ror.org/04vs9wp72 FAU - Li, Wenwei AU - Li W AD - Zhejiang Cancer Hospital, Zhejiang Provincial Key Laboratory of Protein Detection Technology and Diagnostic Equipment, Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou 310022, Zhejiang, People's Republic of China. ROR: https://ror.org/04vs9wp72 FAU - Wang, Mengjie AU - Wang M AD - Zhejiang Cancer Hospital, Zhejiang Provincial Key Laboratory of Protein Detection Technology and Diagnostic Equipment, Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou 310022, Zhejiang, People's Republic of China. ROR: https://ror.org/04vs9wp72 FAU - Dai, Yunlu AU - Dai Y AUID- ORCID: 0000-0003-4023-7320 AD - Cancer Center and Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Macau 999078, China. FAU - Liu, Yuan AU - Liu Y AUID- ORCID: 0009-0006-0515-1811 AD - Zhejiang Cancer Hospital, Zhejiang Provincial Key Laboratory of Protein Detection Technology and Diagnostic Equipment, Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou 310022, Zhejiang, People's Republic of China. ROR: https://ror.org/04vs9wp72 AD - School of Molecular Medicine, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, Zhejiang, China. ROR: https://ror.org/05qbk4x57 LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 RN - 0 (Protein Corona) RN - 0 (Proteome) SB - IM MH - *Proteomics/methods MH - *Nanoparticles/chemistry/metabolism MH - *Protein Corona/chemistry/metabolism MH - Humans MH - Endocytosis MH - Proteome/metabolism MH - Animals OTO - NOTNLM OT - biological identity OT - literature mining OT - molecular regulatory rules OT - nanoparticle protein corona OT - proteomics COIS- Competing interests statement:The authors declare no competing interest. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:44 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:44 [entrez] AID - 10.1073/pnas.2618716123 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2026 Nov 3;123(44):e2618716123. doi: 10.1073/pnas.2618716123. Epub 2026 Oct 29. PMID- 42956032 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1091-6490 (Electronic) IS - 0027-8424 (Linking) VI - 123 IP - 44 DP - 2026 Nov 3 TI - Direct atomic-scale observation reveals rare earth element fractionation at mineral-water interfaces. PG - e2615180123 LID - 10.1073/pnas.2615180123 [doi] AB - Clay minerals, ubiquitous across diverse geological systems, exert fundamental controls on critical geochemical processes, particularly through their adsorptive interactions with economically vital elements such as heavy rare earth elements (HREEs). The adsorption of HREEs onto clay surfaces is not only the key process that enriches these critical resources but also a potential archive for dating significant weathering events. However, the atomic-scale mechanisms governing the preferential adsorption and fractionation of rare earth elements (REEs) at mineral-water interfaces have remained poorly constrained. Here, employing in situ atomic-scale imaging via high-resolution atomic force microscopy, we systematically investigate the adsorption behaviors of light and heavy REEs (La, Y, Yb) onto key crystallographic planes-Si-basal, Al-basal, and edge terminations-of representative clay minerals (kaolinite, illite, mica) under environmentally relevant conditions (pH 4.8 to 6.4). We directly visualize and quantitatively reveal that Si-basal planes selectively enrich HREEs with pH-enhanced adsorption capacity, whereas Al-basal planes exhibit negligible REE adsorption below pH 5.8 but preferential HREE adsorption at pH 6.4. Integrating synchrotron X-ray absorption spectroscopy, we identify the adsorption mechanism of REE on Si-basal and Al-basal/edge planes as outer-sphere complexation and inner-sphere binding, respectively. Furthermore, we quantify the contributions of distinct planes via surface complexation modeling, revealing Si-dominated to Al- and edge-controlled adsorption with elevating pH. These atomic-scale findings not only resolve long-standing debates about clay mineral-mediated REE fractionation but also redefine fundamental paradigms of surface reactivity at silicate mineral-water interfaces, offering insights into the cycling and metallogeny of other critical metals in aqueous and sedimentary environments. FAU - Ran, Lingyu AU - Ran L AUID- ORCID: 0009-0008-0743-8159 AD - State Key Laboratory of Deep Earth Processes and Resources, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. ROR: https://ror.org/034t30j35 AD - Guangdong research center for strategic metals and green utilization, Guangzhou 510640, China. AD - Guangdong Provincial Key Laboratory of Mineral Physics and Material, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. ROR: https://ror.org/034t30j35 AD - College of Earth and Planetary Sciences, College of Earth and Planetary Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. ROR: https://ror.org/05qbk4x57 FAU - Wang, Gaofeng AU - Wang G AUID- ORCID: 0000-0003-0654-6576 AD - State Key Laboratory of Deep Earth Processes and Resources, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. ROR: https://ror.org/034t30j35 AD - Guangdong research center for strategic metals and green utilization, Guangzhou 510640, China. AD - Guangdong Provincial Key Laboratory of Mineral Physics and Material, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. ROR: https://ror.org/034t30j35 AD - College of Earth and Planetary Sciences, College of Earth and Planetary Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. ROR: https://ror.org/05qbk4x57 FAU - Zhu, Jianxi AU - Zhu J AUID- ORCID: 0000-0002-9002-4457 AD - State Key Laboratory of Deep Earth Processes and Resources, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. ROR: https://ror.org/034t30j35 AD - Guangdong research center for strategic metals and green utilization, Guangzhou 510640, China. AD - Guangdong Provincial Key Laboratory of Mineral Physics and Material, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. ROR: https://ror.org/034t30j35 AD - College of Earth and Planetary Sciences, College of Earth and Planetary Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. ROR: https://ror.org/05qbk4x57 FAU - Ling, Bowen AU - Ling B AUID- ORCID: 0000-0002-3218-4807 AD - Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China. ROR: https://ror.org/034t30j35 AD - School of Engineering Science, University of Chinese Academy of Sciences, Beijing 100049, China. ROR: https://ror.org/05qbk4x57 FAU - Xu, Jie AU - Xu J AUID- ORCID: 0009-0005-1370-9005 AD - Zhejiang Key Laboratory of Environment and Health of New Pollutants, School of Environment, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou 310024, China. ROR: https://ror.org/05qbk4x57 FAU - Zhang, Xiangwei AU - Zhang X AD - State Key Laboratory of Deep Earth Processes and Resources, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. ROR: https://ror.org/034t30j35 AD - Guangdong research center for strategic metals and green utilization, Guangzhou 510640, China. AD - Guangdong Provincial Key Laboratory of Mineral Physics and Material, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. ROR: https://ror.org/034t30j35 AD - College of Earth and Planetary Sciences, College of Earth and Planetary Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. ROR: https://ror.org/05qbk4x57 FAU - Liang, Xiaoliang AU - Liang X AD - State Key Laboratory of Deep Earth Processes and Resources, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. ROR: https://ror.org/034t30j35 AD - Guangdong research center for strategic metals and green utilization, Guangzhou 510640, China. AD - Guangdong Provincial Key Laboratory of Mineral Physics and Material, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. ROR: https://ror.org/034t30j35 AD - College of Earth and Planetary Sciences, College of Earth and Planetary Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. ROR: https://ror.org/05qbk4x57 FAU - Yang, Yongqiang AU - Yang Y AUID- ORCID: 0000-0002-3681-4589 AD - State Key Laboratory of Deep Earth Processes and Resources, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. ROR: https://ror.org/034t30j35 AD - Guangdong research center for strategic metals and green utilization, Guangzhou 510640, China. AD - Guangdong Provincial Key Laboratory of Mineral Physics and Material, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. ROR: https://ror.org/034t30j35 AD - College of Earth and Planetary Sciences, College of Earth and Planetary Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. ROR: https://ror.org/05qbk4x57 FAU - Wei, Jingming AU - Wei J AD - State Key Laboratory of Deep Earth Processes and Resources, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. ROR: https://ror.org/034t30j35 AD - Guangdong research center for strategic metals and green utilization, Guangzhou 510640, China. AD - Guangdong Provincial Key Laboratory of Mineral Physics and Material, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. ROR: https://ror.org/034t30j35 AD - College of Earth and Planetary Sciences, College of Earth and Planetary Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. ROR: https://ror.org/05qbk4x57 FAU - He, Hongping AU - He H AUID- ORCID: 0000-0002-7057-7687 AD - State Key Laboratory of Deep Earth Processes and Resources, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. ROR: https://ror.org/034t30j35 AD - Guangdong research center for strategic metals and green utilization, Guangzhou 510640, China. AD - Guangdong Provincial Key Laboratory of Mineral Physics and Material, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China. ROR: https://ror.org/034t30j35 AD - College of Earth and Planetary Sciences, College of Earth and Planetary Sciences, University of Chinese Academy of Sciences, Beijing 100049, China. ROR: https://ror.org/05qbk4x57 LA - eng GR - 92462303/MOST | National Natural Science Foundation of China (NSFC)/ GR - 2025B0303000017/Guangdong Major Project of Basic and Applied Basic Research/ GR - 2024ZD1004002/Deep Earth Probe and Mineral Resources Exploration-National Science and Technology Major Project/ GR - XDA0430205/Strategic Priority Research Program of the Chinese Academy of Sciences/ GR - 42572033/National Natural Science Foundation of China/ GR - 42272158/National Natural Science Foundation of China/ GR - 2024B0303390002/Guangdong S & T Program/ GR - 2024TQ08A283/Guangdong Special Support Program/ GR - 2026ZD1012500/Deep Earth Probe and Mineral Resources Exploration-National Science and Technology Major Project/ PT - Journal Article DEP - 20261029 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 SB - IM OTO - NOTNLM OT - clay mineral OT - in situ high-resolution atomic force microscopy OT - rare earth elements OT - surface reactivity COIS- Competing interests statement:The authors declare no competing interest. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:44 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:44 [entrez] AID - 10.1073/pnas.2615180123 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2026 Nov 3;123(44):e2615180123. doi: 10.1073/pnas.2615180123. Epub 2026 Oct 29. PMID- 42956031 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1091-6490 (Electronic) IS - 0027-8424 (Linking) VI - 123 IP - 44 DP - 2026 Nov 3 TI - Intrinsic function of leptin receptor-regulated endothelial PD-L1. PG - e2609322123 LID - 10.1073/pnas.2609322123 [doi] AB - Programmed cell death ligand-1 (PD-L1) is an immune checkpoint protein expressed in vascular endothelial cells (ECs). In contrast to cancer, our understanding of endothelial PD-L1 in relation to metabolic syndromes and its vascular complications is less studied. For example, its regulation and intrinsic function remain unclear. Here, we showed that endothelial PD-L1 is regulated by leptin receptor signaling in male mice. In particular, endothelial PD-L1 expression is affected by leptin resistance. A lack of leptin receptors leads to PD-L1 downregulation in vascular ECs via posttranslational degradation. To understand the fundamental roles of endothelial PD-L1, RNA interference and selective knockout are employed in human endothelium and animals, respectively. Silencing or deleting endothelial PD-L1 expression delays cell cycle progression and proliferation due to rat sarcoma (RAS)/mitogen-activated protein kinase (MAPK) inactivation. In obesity, endothelial PD-L1 deficiency impairs the EC turnover and worsens the vascular function. Overall, we provide an insight into how endothelial PD-L1 contributes to vascular homeostasis, suggesting its pathophysiological importance in maintaining cellular turnover. FAU - Cheung, Yiu Ming AU - Cheung YM AUID- ORCID: 0000-0001-7366-3882 AD - School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China. ROR: https://ror.org/00t33hh48 AD - State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China. ROR: https://ror.org/00t33hh48 FAU - Yeung, Hoi Wa AU - Yeung HW AUID- ORCID: 0009-0000-3660-3354 AD - School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China. ROR: https://ror.org/00t33hh48 AD - State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China. ROR: https://ror.org/00t33hh48 FAU - He, Chufeng AU - He C AUID- ORCID: 0009-0004-2195-6615 AD - School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China. ROR: https://ror.org/00t33hh48 AD - State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China. ROR: https://ror.org/00t33hh48 FAU - Zhang, Qile AU - Zhang Q AUID- ORCID: 0009-0001-1053-4386 AD - School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China. ROR: https://ror.org/00t33hh48 AD - State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China. ROR: https://ror.org/00t33hh48 FAU - Chook, Chui Yiu Bamboo AU - Chook CYB AUID- ORCID: 0000-0002-1366-6990 AD - School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China. ROR: https://ror.org/00t33hh48 FAU - Tse, Gary AU - Tse G AD - Department of Population Health, School of Nursing and Health Sciences, Hong Kong Metropolitan University, Hong Kong Special Administrative Region, China. ROR: https://ror.org/0349bsm71 FAU - Liao, Yun AU - Liao Y AD - Department of Clinical Pharmacy, Shanghai General Hospital, Shanghai 200080, China. ROR: https://ror.org/04a46mh28 FAU - Wong, Wing Tak AU - Wong WT AUID- ORCID: 0000-0002-4514-3780 AD - School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China. ROR: https://ror.org/00t33hh48 AD - State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, China. ROR: https://ror.org/00t33hh48 LA - eng GR - 14105022/Research Grant Council, University Grant Committee/ GR - 24B2/029A/Health Bureau ()/ GR - 8300068/State Key Laboratory of Agrobiotechnology/ GR - 8300041/State Key Laboratory of Agrobiotechnology/ PT - Journal Article DEP - 20261029 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 RN - 0 (B7-H1 Antigen) RN - 0 (Receptors, Leptin) RN - 0 (Cd274 protein, mouse) RN - 0 (CD274 protein, human) RN - 0 (Leptin) RN - 0 (leptin receptor, mouse) SB - IM MH - Animals MH - *B7-H1 Antigen/metabolism/genetics MH - *Receptors, Leptin/metabolism/genetics MH - Humans MH - Male MH - *Endothelial Cells/metabolism MH - Mice MH - Signal Transduction MH - Mice, Knockout MH - Cell Proliferation MH - Obesity/metabolism MH - Leptin/metabolism MH - *Endothelium, Vascular/metabolism OTO - NOTNLM OT - PD-L1 OT - endothelial dysfunction OT - leptin receptor OT - obesity COIS- Competing interests statement:The authors declare no competing interest. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:44 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:44 [entrez] AID - 10.1073/pnas.2609322123 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2026 Nov 3;123(44):e2609322123. doi: 10.1073/pnas.2609322123. Epub 2026 Oct 29. PMID- 42956030 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1091-6490 (Electronic) IS - 0027-8424 (Linking) VI - 123 IP - 44 DP - 2026 Nov 3 TI - A sulfotyrosine motif in the human TrkB extracellular domain is required for activation by the agonist antibody ZEB85. PG - e2618780123 LID - 10.1073/pnas.2618780123 [doi] AB - The brain-derived neurotrophic factor (BDNF)-tropomyosin receptor kinase B (TrkB) signaling axis is a key effector of synaptic plasticity and neuroprotection. While TrkB activation is a major target to prevent neural dysfunction, stimulation with exogenous BDNF administration has not proven tractable given the unfavorable physiochemical properties of BDNF. In addition, BDNF also activates a cell death pathway by binding to the neurotrophin receptor p75. The TrkB agonist antibody ZEB85 provides an alternative route to the selective activation of TrkB. To understand the ZEB85 mechanism of action, we mapped the binding site for ZEB85 to the TrkB extracellular juxtamembrane domain and found a sulfotyrosine modification at residue 400 crucial for ZEB85 interaction and activation of TrkB signaling. We determined the structures of ZEB85 and BDNF bound to their respective binding sites on human TrkB suggesting independent sites for each ligand, despite prior evidence of direct competition. Using structure-guided BDNF- and ZEB85-binding deficient TrkB mutants, we assessed their ability to sequester ligands from full-length TrkB in cultured human neurons. We found that the BDNF binding site extends into the juxtamembrane domain of TrkB but does not require the sulfotyrosine 400 to activate TrkB. Together with biophysical analysis and AlphaFold modeling, these results explain how BDNF can displace ZEB85 from TrkB through an overlapping epitope. Our findings reveal unique features of TrkB, not present in the related neurotrophin receptors TrkA and TrkC and suggest future directions to explore the role of sulfotyrosine in TrkB signaling. FAU - Briggs, David C AU - Briggs DC AUID- ORCID: 0000-0002-9793-7339 AD - Signalling and Structural Biology Lab, The Francis Crick Institute, London NW1 1AT, United Kingdom. ROR: https://ror.org/04tnbqb63 FAU - Duffy, Ryan T AU - Duffy RT AUID- ORCID: 0009-0001-3440-9731 AD - Centre of Membrane Proteins and Receptors, School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, United Kingdom. ROR: https://ror.org/01ee9ar58 FAU - Ateaque, Sarah AU - Ateaque S AD - School of Biosciences, Cardiff University, Cardiff CF10 3AX, Wales, United Kingdom. ROR: https://ror.org/03kk7td41 FAU - Maslen, Sarah AU - Maslen S AD - Proteomics Science Technology Platform, The Francis Crick Institute, London NW1 1AT, United Kingdom. ROR: https://ror.org/04tnbqb63 FAU - Nagaraj, Hema AU - Nagaraj H AD - Chemical Biology Science Technology Platform, The Francis Crick Institute, London NW1 1AT, United Kingdom. ROR: https://ror.org/04tnbqb63 FAU - Barde, Yves-Alain AU - Barde YA AUID- ORCID: 0000-0002-7627-461X AD - School of Biosciences, Cardiff University, Cardiff CF10 3AX, Wales, United Kingdom. ROR: https://ror.org/03kk7td41 FAU - DiStefano, Peter S AU - DiStefano PS AUID- ORCID: 0009-0000-9042-2589 AD - Zebra Biologics Inc, Concord, MA 01742. FAU - Lindsay, Ronald M AU - Lindsay RM AUID- ORCID: 0000-0001-8377-5079 AD - Zebra Biologics Inc, Concord, MA 01742. FAU - Armstrong, Paul C AU - Armstrong PC AUID- ORCID: 0000-0003-0904-677X AD - Blizard Institute, The Faculty of Medicine and Dentistry, Queen Mary University of London, London E1 2AT, United Kingdom. ROR: https://ror.org/026zzn846 FAU - Peach, Chloe J AU - Peach CJ AD - Centre of Membrane Proteins and Receptors, School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, United Kingdom. ROR: https://ror.org/01ee9ar58 FAU - McDonald, Neil Q AU - McDonald NQ AUID- ORCID: 0000-0003-0975-6325 AD - Signalling and Structural Biology Lab, The Francis Crick Institute, London NW1 1AT, United Kingdom. ROR: https://ror.org/04tnbqb63 AD - Institute of Structural and Molecular Biology, School of Natural Sciences, Birkbeck College, London WC1E 7HX, United Kingdom. ROR: https://ror.org/02mb95055 LA - eng GR - CC2068/Francis Crick Institute (FCI)/ PT - Journal Article DEP - 20261029 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 RN - EC 2.7.10.1 (Receptor, trkB) RN - 42HK56048U (Tyrosine) RN - 0 (Brain-Derived Neurotrophic Factor) RN - EC 2.7.10.1 (tropomyosin-related kinase-B, human) RN - 29166358BF (tyrosine O-sulfate) RN - 0 (Membrane Glycoproteins) RN - 7171WSG8A2 (BDNF protein, human) SB - IM MH - Humans MH - *Receptor, trkB/agonists/chemistry/metabolism/genetics MH - *Tyrosine/analogs & derivatives/chemistry/metabolism MH - Brain-Derived Neurotrophic Factor/metabolism/chemistry MH - Binding Sites MH - Protein Domains MH - Signal Transduction MH - *Membrane Glycoproteins/agonists/chemistry/metabolism/genetics MH - Protein Binding OTO - NOTNLM OT - BDNF OT - TrkB OT - agonist OT - neurotrophins OT - sulfotyrosine COIS- Competing interests statement:P.S.D. and R.M.L. are affiliated with Zebra Biologics Inc. This work was in part supported by Zebra Biologics Inc. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:44 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:44 [entrez] AID - 10.1073/pnas.2618780123 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2026 Nov 3;123(44):e2618780123. doi: 10.1073/pnas.2618780123. Epub 2026 Oct 29. PMID- 42956024 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1091-6490 (Electronic) IS - 0027-8424 (Linking) VI - 123 IP - 44 DP - 2026 Nov 3 TI - The efficiency of information gain by natural selection. PG - e2611968123 LID - 10.1073/pnas.2611968123 [doi] AB - Natural selection enriches populations for genotypes that are well adapted to their environments, accumulating information about the selective conditions in the process. Therefore, selection can be viewed as a learning process through which populations acquire information about the environment. Motoo Kimura derived a relationship between the amount of information gained by selection and a measure of the inefficiency of the selective process, known as substitution load [M. Kimura, Genet. Res. 2, 127-140 (1961)]. Due to the gradual nature of selection and the ongoing mismatch of types to the environment, a lineage that is still acquiring information has lower mean fitness than a lineage that already has it. While this has been an influential insight, we find that Kimura's model is inconsistent with selection experiments involving Escherichia coli. To address limitations in this model and develop a more robust measure for the efficiency of selection, we turn to computational learning theory. We show that the learning-theoretic concept of regret quantifies the inefficiency of selection as a learning process in terms of relative lineage fitness. We establish bounds on the efficiency of information gain in general settings, including frequency-dependent selection and arbitrary environmental change. We experimentally validate these bounds and demonstrate that our extended theory recovers Kimura's fundamental insight. Finally, we show that selection is an asymptotically optimal algorithm for the basic problem faced by evolving populations and that it acquires information more efficiently than comparable processes in complex environments. FAU - McGee, Ryan Seamus AU - McGee RS AUID- ORCID: 0000-0002-5142-7552 AD - Department of Neuroscience, Washington University School of Medicine in St. Louis, St. Louis, MO 63110. ROR: https://ror.org/01yc7t268 FAU - Kosterlitz, Olivia AU - Kosterlitz O AUID- ORCID: 0000-0002-8692-9595 AD - Department of Biological Sciences, University of Idaho, Moscow, ID 83844. ROR: https://ror.org/03hbp5t65 FAU - Kaznatcheev, Artem AU - Kaznatcheev A AD - Department of Mathematics, Utrecht University, Utrecht 3508 TA, The Netherlands. ROR: https://ror.org/04pp8hn57 AD - Department of Information and Computing Sciences, Utrecht University, Utrecht 3584 CC, The Netherlands. ROR: https://ror.org/04pp8hn57 FAU - Kerr, Benjamin AU - Kerr B AUID- ORCID: 0000-0002-9909-7070 AD - Department of Biology, University of Washington, Seattle, WA 98195-1800. ROR: https://ror.org/00cvxb145 FAU - Bergstrom, Carl T AU - Bergstrom CT AUID- ORCID: 0000-0002-2070-385X AD - Department of Biology, University of Washington, Seattle, WA 98195-1800. ROR: https://ror.org/00cvxb145 AD - Santa Fe Institute, Santa Fe, NM 87501. ROR: https://ror.org/01arysc35 LA - eng GR - DGE-2140004/NSF | National Science Foundation Graduate Research Fellowship Program (GRFP)/ GR - 20650/John Templeton Foundation (JTF)/ GR - DGE-1762114/NSF | National Science Foundation Graduate Research Fellowship Program (GRFP)/ PT - Journal Article DEP - 20261029 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 SB - IM MH - *Selection, Genetic MH - Escherichia coli/genetics MH - *Models, Genetic OTO - NOTNLM OT - evolutionary game theory OT - information theory OT - learning theory OT - natural selection COIS- Competing interests statement:The authors declare no competing interest. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:44 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:44 [entrez] AID - 10.1073/pnas.2611968123 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2026 Nov 3;123(44):e2611968123. doi: 10.1073/pnas.2611968123. Epub 2026 Oct 29. PMID- 42956023 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Study on the molecular mechanism of chlorotoxin from Buthus martensii Karsch in the inhibition of glioma angiogenesis. PG - e0361186 LID - 10.1371/journal.pone.0361186 [doi] AB - BACKGROUND: Glioma is a highly malignant intracranial tumor with dismal prognosis. Tumor angiogenesis critically drives glioma growth and invasion, making vascular endothelial cells a promising therapeutic target. Buthus martensii Karsch chlorotoxin (BmK CT) presents favorable antitumor properties, whereas its regulatory role in glioma angiogenesis and the specific mechanism remain poorly defined. AIM: To investigate the regulatory role of BmK CT in glioma angiogenesis and the underlying mechanism. METHODS: HUVECs were cultured alone or exposed to glioma conditioned medium prepared from primary glioma cells. Functional assays were performed to assess the impacts of BmK CT on endothelial proliferation, migration and tube formation. Quantitative proteomics and bioinformatics analysis were applied to screen differentially expressed proteins (DEPs) and explore underlying mechanisms. RESULTS: BmK CT significantly inhibited the proliferation of HUVEC cells and reduced their migration and tube formation abilities. BmK CT reversed the promoting effect of primary glioma cells on the migration of HUVEC cells. Proteomics identified multiple DEPs. Enrichment analysis suggested that BmK CT exerts an anti-glioma angiogenesis effect through a multi-target mechanism by inhibiting pro-angiogenic pathways, blocking energy metabolism, and regulating the inflammatory microenvironment. Furthermore, expression pattern clustering analysis revealed six DEPs expression patterns. Parallel reaction monitoring (PRM) analysis supported nine candidate proteins (THBS1, THBS4, COMP, F2, A2M, C3, RIGI, EZR, and ITGA6) associated with glioma angiogenesis. Conclusion: This study demonstrated in vitro that BmK CT inhibits endothelial angiogenic behaviors relevant to glioma progression and modulates the tumor microenvironment, and identified nine candidate DEPs, providing a theoretical basis for future molecular targeted therapy strategies for glioma. CI - Copyright: (c) 2026 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Wang, Yiming AU - Wang Y AD - Department of Neurosurgery, Heyou Hospital, Foshan, China. AD - Department of Neurosurgery, Shanxi Provincial People's Hospital, Taiyuan, China. FAU - Zhao, Yaning AU - Zhao Y AD - Department of Neurosurgery, Shanxi Provincial People's Hospital, Taiyuan, China. AD - Department of Neurosurgery, Xi'an International Medical Center Hospital, Xian, China. FAU - Ren, Ziyang AU - Ren Z AD - Department of Neurosurgery, Shanxi Provincial People's Hospital, Taiyuan, China. FAU - Chen, Yiran AU - Chen Y AD - Department of Neurosurgery, Shanxi Provincial People's Hospital, Taiyuan, China. FAU - Chen, Liangchong AU - Chen L AD - Department of Neurosurgery, Shanxi Provincial People's Hospital, Taiyuan, China. AD - Department of Neurosurgery, Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine affiliated to Zhejiang Chinese Medical University, Wenzhou, China. FAU - Hu, Tao AU - Hu T AUID- ORCID: 0009-0003-0478-0657 AD - Department of Neurosurgery, Shanxi Provincial People's Hospital, Taiyuan, China. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 06UV5RFW57 (Chlorotoxin) RN - 0 (Scorpion Venoms) RN - 0 (Hydrocarbons, Chlorinated) RN - Mesobuthus martensii SB - IM MH - Humans MH - *Glioma/pathology/drug therapy/metabolism/blood supply MH - *Scorpion Venoms/pharmacology MH - *Neovascularization, Pathologic/drug therapy/metabolism/pathology MH - Human Umbilical Vein Endothelial Cells/drug effects MH - Cell Movement/drug effects MH - Cell Proliferation/drug effects MH - Animals MH - *Hydrocarbons, Chlorinated/pharmacology MH - Proteomics MH - *Brain Neoplasms/pathology/drug therapy/blood supply/metabolism MH - Cell Line, Tumor MH - Animals, Poisonous MH - Scorpions COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:44 PHST- 2026/07/09 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:44 [entrez] AID - PONE-D-26-32891 [pii] AID - 10.1371/journal.pone.0361186 [doi] PST - epublish SO - PLoS One. 2026 Oct 29;21(10):e0361186. doi: 10.1371/journal.pone.0361186. eCollection 2026. PMID- 42956008 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Eating behaviour and glycaemic control: A cross-sectional study in adolescents with diabetes. PG - e0361574 LID - 10.1371/journal.pone.0361574 [doi] AB - BACKGROUND: Achieving early glycemic control in adolescents with diabetes is associated with improved short- and long-term health outcomes. The aim of this study was to examine the relationship between eating attitudes and glycemic control, as well as the factors associated with eating attitudes and glycemic control, in adolescents with diabetes. METHODS: This descriptive cross-sectional study included 143 adolescents with diabetes, aged 10-19 years, who attended a research hospital between February and April 2024. The data were collected using a Descriptive Information Form, Eating Attitudes Test (EAT) and an information form questioning the values of some metabolic variables. RESULTS: The mean age of the participants was 14 +/- 2.3 years and 49.7% had diabetes between 1-5 years. The mean total score of the EAT scale was 16.62 +/- 8.30. The mean score of the "Dieting" subscale of the EAT scale was 10.39 +/- 5.58, the mean score of the "Bulimia food preoccupation" subscale was 2.33 +/- 3.03 and the mean score of the "Oral Control" subscale was 3.79 +/- 3.08. There was no significant difference between the mean EAT total score and glycemic control. CONCLUSIONS: The mean EAT-26 total score was below the commonly used cutoff score, and no significant association was found between EAT-26 scores and HbA1c. Differences in EAT-26 total and subscale scores were observed according to selected demographic and clinical characteristics, including BMI, treatment modality, and treatment adherence. These findings describe self-reported eating attitudes in the study sample and support the inclusion of eating-related assessment within the holistic care of adolescents with Type 1 DM. CI - Copyright: (c) 2026 Simsekoglu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Simsekoglu, Nesibe AU - Simsekoglu N AUID- ORCID: 0000-0002-6461-7961 AD - Department of Fundamentals of Nursing, Hamidiye Faculty of Nursing, University of Health Sciences, Istanbul, Turkiye. FAU - Arslanoglu, Ilknur AU - Arslanoglu I AUID- ORCID: 0000-0002-4975-9718 AD - Department of Pediatric Endocrinology, Research Hospital, Duzce University Faculty of Medicine, Duzce, Turkiye. FAU - Portakal, Banu AU - Portakal B AD - Diabetes Polyclinic, Research Hospital, Duzce University Faculty of Medicine, Duzce, Turkiye. FAU - Aktas, Selman AU - Aktas S AD - Biostatistics Department, Hamidiye Medical Faculty, University of Health Sciences, Istanbul, Turkiye. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (Blood Glucose) RN - 0 (Glycated Hemoglobin) SB - IM MH - Humans MH - Adolescent MH - Cross-Sectional Studies MH - Female MH - Child MH - Male MH - *Glycemic Control MH - *Feeding Behavior/psychology MH - Young Adult MH - Blood Glucose MH - *Diabetes Mellitus, Type 1/blood/psychology MH - Glycated Hemoglobin/analysis COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:43 PHST- 2026/05/13 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:43 [entrez] AID - PONE-D-26-18898 [pii] AID - 10.1371/journal.pone.0361574 [doi] PST - epublish SO - PLoS One. 2026 Oct 29;21(10):e0361574. doi: 10.1371/journal.pone.0361574. eCollection 2026. PMID- 42956000 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Rip current detection in nearshore areas through UAV video analysis using optical flow and almost local-isometric embedding techniques on the sphere. PG - e0360672 LID - 10.1371/journal.pone.0360672 [doi] AB - Rip currents cause most surf-beach lifeguard rescues and most beach drowning incidents, yet automated detection relies on costly instrumentation that scales poorly across the many beaches that lack lifeguards. We propose an end-to-end pipeline producing pixel-level rip-current likelihood maps directly from raw unmanned aerial vehicle (UAV) video without manual ground-control points - to our knowledge the first to do so from uncalibrated drone video, prior automated approaches operating at video-clip granularity or requiring surveyed camera installations. The pipeline composes (i) dense optical-flow estimation with five classical algorithm variants; (ii) a sphere-aware projection mapping pixel coordinates to local-isometric ground coordinates, with a tilt-aware Jacobian correction of per-pixel velocity; (iii) a CNN six-class scene parser (sky/sea/land/foam/structure/void), trained on frames verified by dense pixel-matching to contain no extraction from either evaluation video; (iv) a per-pixel temporal accumulator flagging pixels whose flow direction agrees with the locally-estimated offshore direction within +/-90 composite function - the physical boundary of seaward-directed flow, shown robust in an 18-scene sensitivity sweep; and (v) post-processing by Gaussian smoothing (sigma=25 px) and a normalized cross-algorithm ensemble. We evaluate on two UAV-captured rip-current videos at Palm Beach and Haulover Park, Florida, annotated by consensus among three coastal-science domain experts. At the physically-neutral T=90 composite function default the framework attains precision-recall AUC 0.636 at Palm Beach and 0.660 at Haulover; per-scene selection - of the angular tolerance, ensemble membership, mixing weight, sphere tilt, and which modules are engaged - against the same expert annotation used for evaluation raises these to 0.7066 and 0.7040, reported as an optimistic upper bound rather than an expected-performance estimate. We release the source code, the trained sea-mask refiner, a YOLOv8m-seg deep-learning baseline, and the evaluation scripts. Evaluation is limited to these two expert-annotated scenes, and the method requires visible surface tracers (foam, dye, or sediment fronts) advected by the current. CI - Copyright: (c) 2026 Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Yang, Kaiqi AU - Yang K AD - Department of Mathematics, University of Miami, Coral Gables, Florida, United States of America. FAU - Leatherman, Stephen B AU - Leatherman SB AD - Department of Earth and Environment, Florida International University, Miami, Florida, United States of America. FAU - Sun, Anchen AU - Sun A AUID- ORCID: 0000-0003-3633-6385 AD - Department of Electrical and Computer Engineering, University of Miami, Coral Gables, Florida, United States of America. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - *Video Recording/methods MH - *Unmanned Aerial Devices MH - Algorithms MH - *Image Processing, Computer-Assisted/methods MH - Detection Algorithms MH - Bathing Beaches MH - Convolutional Neural Networks COIS- NO authors have competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:43 PHST- 2025/04/15 00:00 [received] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:43 [entrez] AID - PONE-D-25-20427 [pii] AID - 10.1371/journal.pone.0360672 [doi] PST - epublish SO - PLoS One. 2026 Oct 29;21(10):e0360672. doi: 10.1371/journal.pone.0360672. eCollection 2026. PMID- 42955998 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Is Gen AI an editor or a revision tool? A case study of EFL students in AI-assisted revision. PG - e0359661 LID - 10.1371/journal.pone.0359661 [doi] AB - As generative artificial intelligence (Gen AI) tools become increasingly integrated into language education, understanding how learners respond to AI-generated feedback during writing revision has become an important research concern. Existing studies have primarily examined the effects of AI on writing performance, with less attention to learners' reported decision-making and reflection during revision. This qualitative study investigated learner-AI interaction, reported revision practices and learner perceptions among 18 first-year non-English-major students enrolled in a university EFL writing course. Data were collected through semi-structured interviews following a Gen AI-assisted revision task and analysed using thematic analysis. The findings identified three interaction patterns with Gen AI: passive AI reliance, adaptive AI engagement, metacognitive AI engagement. Participants differed in how they evaluated and used AI-generated feedback, including passive acceptance, selective uptake, and critical evaluation. Some reported increased confidence, motivation, and awareness of linguistic form, whereas others expressed concerns about overreliance, authorship, and loss of personal voice. These self-reported experiences provide insight into how EFL learners perceive and manage GenAI-assisted revision, highlighting the need for pedagogical approaches that foster critical AI literacy and reflective learner autonomy. CI - Copyright: (c) 2026 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Zhang, Han AU - Zhang H AUID- ORCID: 0000-0002-7275-0631 AD - Hebei Normal University, Shijiazhuang, Hebei, China. AD - University of Malaya, Kuala Lumpur, Malaysia. FAU - Ai, Shanshan AU - Ai S AD - Hebei Minzu Normal University, Chengde, Hebei, China. FAU - Zhang, Lingyu AU - Zhang L AUID- ORCID: 0009-0008-1791-5078 AD - Hebei Minzu Normal University, Chengde, Hebei, China. FAU - Lin, Yuhui AU - Lin Y AD - Hebei Minzu Normal University, Chengde, Hebei, China. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Humans MH - *Generative Artificial Intelligence MH - *Students/psychology MH - *Writing MH - Female MH - Male MH - Learning MH - Young Adult MH - Language COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:43 PHST- 2025/09/17 00:00 [received] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:43 [entrez] AID - PONE-D-25-49050 [pii] AID - 10.1371/journal.pone.0359661 [doi] PST - epublish SO - PLoS One. 2026 Oct 29;21(10):e0359661. doi: 10.1371/journal.pone.0359661. eCollection 2026. PMID- 42955993 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - A target-domain augmentation and class-wise alignment framework for few-shot cross-condition bearing fault diagnosis. PG - e0361486 LID - 10.1371/journal.pone.0361486 [doi] AB - Cross-condition bearing fault diagnosis often has to adapt to a new operating condition with only a few labelled target samples. This setting creates two coupled problems. First, the sparse target support set cannot describe the target fault distribution sufficiently. Second, the same fault class may occupy different feature regions across source and target conditions, which weakens source-domain pretrained classifiers. Existing methods usually improve few-shot learning or reduce domain shift, but they rarely enrich the labelled target-class representation before class-wise source-target alignment. To address this limitation, this paper proposes a target-domain augmentation and class-wise alignment framework for few-shot cross-condition bearing fault diagnosis. The rime optimization algorithm (RIME) is first used to optimize the variational mode decomposition (VMD) parameters K and alpha, and fault-sensitive components are selected using envelope entropy and adjacent modal-frequency overlap. The reconstructed signal is converted into symmetric dot pattern (SDP) images to provide a noise-suppressed polar representation. The fast generative adversarial network (FastGAN) then generates auxiliary target-domain SDP samples from the limited labelled target support set, making each target fault class less sparse. On this basis, class-wise maximum mean discrepancy (CMMD) is embedded into a Swin Transformer classifier to align source and target features within each known fault category. Experiments on the Case Western Reserve University (CWRU) and Xi'an Jiaotong University-Shenyang Blower Works (XJTU-SY) datasets show that the proposed framework improves target-domain few-shot adaptation, achieving 93.33% and 97.58% accuracy under the G20 and G50 XJTU-SY target-augmentation settings, respectively. CI - Copyright: (c) 2026 Hu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Hu, Yijia AU - Hu Y AD - Industrial Technology Center, North China Institute of Aerospace Engineering, Langfang, China. AD - Hebei Province Technology Innovation Center for Aerospace Composite Structural Design and Manufacturing, North China Institute of Aerospace Engineering, Langfang, China. FAU - Gu, Ying AU - Gu Y AD - School of Mechanical and Electrical Engineering, North China Institute of Aerospace Engineering, Langfang, China. FAU - Su, Yanjie AU - Su Y AD - Industrial Technology Center, North China Institute of Aerospace Engineering, Langfang, China. AD - Hebei Province Technology Innovation Center for Aerospace Composite Structural Design and Manufacturing, North China Institute of Aerospace Engineering, Langfang, China. FAU - Zhao, Chuan AU - Zhao C AUID- ORCID: 0000-0003-0226-3050 AD - School of Mechanical and Electrical Engineering, North China Institute of Aerospace Engineering, Langfang, China. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - *Algorithms MH - Generative Adversarial Networks COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:42 PHST- 2026/07/12 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:42 [entrez] AID - PONE-D-26-34314 [pii] AID - 10.1371/journal.pone.0361486 [doi] PST - epublish SO - PLoS One. 2026 Oct 29;21(10):e0361486. doi: 10.1371/journal.pone.0361486. eCollection 2026. PMID- 42955981 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1998-4138 (Electronic) IS - 1998-4138 (Linking) VI - 22 IP - 5 DP - 2026 Jul 1 TI - From imaging to insight: Assessing the diagnostic performance of MRI in endometrial cancer staging. PG - 974-979 LID - 10.4103/jcrt.jcrt_1924_25 [doi] AB - BACKGROUND: Endometrial carcinoma (EC), one of the most common malignancies of the female reproductive tract, predominantly affects postmenopausal women. The incidence of EC has been rising over the past few decades, partly due to increasing rates of obesity, diabetes, and an aging population. A variety of diagnostic techniques exist to assess and diagnose endometrial carcinoma, including histopathology and imaging modalities such as ultrasonography, computed tomography (CT), and magnetic resonance imaging (MRI). MRI can provide valuable information about the anatomical characteristics of the tumor, such as the depth of myometrial invasion, cervical involvement, and the presence of lymph node or distant metastases, which can help guide treatment decisions. MATERIAL AND METHODS: A total of 50 biopsy proven cases of endometrial carcinoma were enrolled in the study and subjected to MRI. After the staging laparotomy, the comparison of preoperative MRI with histopathological findings was done. RESULTS: MRI showed a very good correlation with HPE for all the 3 parameters with accuracy of 90% for myometrial invasion, 72% for cervical involvement, and 90% for lymph node metastasis. CONCLUSION: MRI provides excellent soft tissue contrast and detailed visualization. MRI's noninvasive nature and high sensitivity makes it an invaluable diagnostic tool for preoperative staging, guiding clinical decision-making and treatment planning. CI - Copyright (c) 2026 Journal of Cancer Research and Therapeutics. FAU - Sharma, Deeksha AU - Sharma D AD - Department of Obstetrics and Gynaecology, Guru Gobind Singh Medical College and Hospital, Faridkot, Punjab, India. FAU - Goyal, Lajya D AU - Goyal LD AD - Department of Obstetrics and Gynaecology, All India Institute of Medical Sciences, Bathinda, Punjab, India. FAU - Rajora, Parveen AU - Rajora P AD - Department of Obstetrics and Gynaecology, Guru Gobind Singh Medical College and Hospital, Faridkot, Punjab, India. FAU - Singh, Paramdeep AU - Singh P AD - Department of Radiology, All India Institute of Medical Sciences, Bathinda, Punjab, India. FAU - Kaur, Khushpreet AU - Kaur K AD - Department of Obstetrics and Gynaecology, All India Institute of Medical Sciences, Bathinda, Punjab, India. LA - eng PT - Journal Article DEP - 20261029 PL - India TA - J Cancer Res Ther JT - Journal of cancer research and therapeutics JID - 101249598 SB - IM MH - Humans MH - Female MH - *Endometrial Neoplasms/pathology/diagnostic imaging/surgery/diagnosis MH - *Magnetic Resonance Imaging/methods MH - Neoplasm Staging MH - Middle Aged MH - Aged MH - Lymphatic Metastasis/diagnostic imaging MH - Adult MH - Ultrasonography MH - Tomography, X-Ray Computed MH - Myometrium/pathology/diagnostic imaging MH - Neoplasm Invasiveness OTO - NOTNLM OT - Diagnostic accuracy OT - MRI OT - endometrial carcinoma OT - histopathological findings OT - invasion EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:36 PHST- 2025/09/08 00:00 [received] PHST- 2026/08/10 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:36 [entrez] AID - 01363817-202610000-00027 [pii] AID - 10.4103/jcrt.jcrt_1924_25 [doi] PST - ppublish SO - J Cancer Res Ther. 2026 Jul 1;22(5):974-979. doi: 10.4103/jcrt.jcrt_1924_25. Epub 2026 Oct 29. PMID- 42955980 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1998-4138 (Electronic) IS - 1998-4138 (Linking) VI - 22 IP - 5 DP - 2026 Jul 1 TI - Clinicopathological features, prognostic factors and treatment outcome in male breast cancer: An observational retrospective study from tertiary cancer center. PG - 969-973 LID - 10.4103/jcrt.jcrt_2500_25 [doi] AB - BACKGROUND: Male breast cancer (MBC) is a rare malignancy, accounting for less than 1% of all breast cancers worldwide. Limited awareness among patients and clinicians often results in delayed diagnosis and advanced stage at presentation. Data from India remain scarce. This study aimed to evaluate the clinic pathological profile, treatment patterns, and outcomes of MBC patients at our tertiary cancer institute. METHODOLOGY: A retrospective observational study was conducted at the Government Medical College and State Cancer Institute, Amritsar, from 2014 to 2023. Sixteen histologically confirmed MBC cases were analyzed for demographics, clinical presentation, pathological findings, treatment details, and outcomes. Diagnosis was confirmed by FNAC/biopsy, and hormone receptor status was assessed by immunohistochemistry. Staging was performed according to the AJCC 8 th edition. RESULTS: The mean age was 59 years (median: 56; range: 39-74). The most common presentation was a breast lump (62.5%), followed by skin changes and inflammatory lesions (18.75% each). Left-sided involvement was predominant (87.5%), with the central quadrant most frequently affected (50%). At the time of diagnosis, 43.75% had stage II, 37.5% stage III, and 18.75% metastatic disease. Invasive ductal carcinoma was the predominant histology (75%), with hormone receptor positivity in 56.25%. Modified radical mastectomy was performed in nine patients and chemotherapy was given to 81.25%. Hormonal therapy was prescribed in 56.25% cases. The mean overall survival was 52.5 months. Patients with advanced disease had median survival of 16.4 months. Out of 16 patients, six died of disease with a median survival of 16.4 months (interquartile range, IQR): 13.3-58.0; range: 13.1-66.6 months). Remaining 10 patients who presented early and underwent treatment, had median survival duration of 67.3 months (IQR: 30.3-98.6; range: 6.6-115.5 months). CONCLUSION: MBC in India presents at a relatively younger age and more advanced stage compared to Western cohorts. Survival is compromised by delayed diagnosis, social stigma, and treatment noncompliance. Greater awareness, timely detection, and standardized multimodality care are essential to improve outcomes. CI - Copyright (c) 2026 Journal of Cancer Research and Therapeutics. FAU - Sunigdha AU - Sunigdha AD - Department of Radiation Oncology, Government Medical College and Rajindra Hospital, Patiala, Punjab, India. FAU - Thakur, Niketa AU - Thakur N AD - Department of Radiation Oncology, All India Institute of Medical Sciences, Bilaspur, Himachal Pradesh, India. AD - Department of Radiation Oncology, Government Medical College, Amritsar, Punjab, India. FAU - Singh, Ramandeep AU - Singh R AD - Department of Radiation Oncology, Advanced Cancer Institute, Bathinda, Punjab, India. FAU - Devgan, Rajiv AU - Devgan R AD - Department of Radiation Oncology, Government Medical College, Amritsar, Punjab, India. FAU - Sharma, Gunjan AU - Sharma G AD - Department of Radiation Oncology, Guru Nanak Dev Medical College and Hospital, Amritsar, Punjab, India. FAU - Singh, K Manraj AU - Singh KM AD - Department of Radiation Oncology, Government Medical College, Amritsar, Punjab, India. LA - eng PT - Journal Article PT - Observational Study DEP - 20261029 PL - India TA - J Cancer Res Ther JT - Journal of cancer research and therapeutics JID - 101249598 SB - IM MH - Humans MH - *Breast Neoplasms, Male/pathology/therapy/diagnosis/mortality MH - Male MH - Retrospective Studies MH - Middle Aged MH - Tertiary Care Centers/statistics & numerical data MH - Prognosis MH - Aged MH - Adult MH - India/epidemiology MH - Neoplasm Staging MH - Treatment Outcome OTO - NOTNLM OT - Invasive breast cancer OT - male breast cancer OT - rare EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:36 PHST- 2025/12/18 00:00 [received] PHST- 2026/08/10 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:36 [entrez] AID - 01363817-202610000-00026 [pii] AID - 10.4103/jcrt.jcrt_2500_25 [doi] PST - ppublish SO - J Cancer Res Ther. 2026 Jul 1;22(5):969-973. doi: 10.4103/jcrt.jcrt_2500_25. Epub 2026 Oct 29. PMID- 42955971 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1998-4138 (Electronic) IS - 1998-4138 (Linking) VI - 22 IP - 5 DP - 2026 Jul 1 TI - Luminal B-like predominance in breast cancer: A 5-year institutional experience of 2271 women from a tertiary cancer center in South India. PG - 903-910 LID - 10.4103/jcrt.jcrt_2072_25 [doi] AB - INTRODUCTION: Breast cancer is the most frequently diagnosed malignancy among women, surpassing cervical cancer. Classifying tumors based on surrogate molecular subtypes using immunohistochemistry (IHC) and in situ hybridization (ISH) for human epidermal growth factor receptor (HER2) is essential for guiding treatment decisions. This study aims to assess the prevalence of these molecular subtypes in a South Indian population. MATERIALS AND METHODS: This retrospective study included women diagnosed with invasive breast carcinoma between January 2019 and December 2023. Cases with incomplete biomarker data were excluded. IHC for estrogen receptor (ER), progesterone receptor (PR), HER2, and Ki-67 was performed, and HER2 IHC 2 + cases were further evaluated using fluorescence in situ hybridization. Tumors were categorized into surrogate molecular subtypes using established American Society of Clinical Oncology and College of American Pathologists and St. Gallen guidelines. Clinical and histopathological data were collected from hospital records. RESULTS: A total of 2271 patients were analyzed (median age: 58 years; 73.6% postmenopausal). Luminal B-like tumors were the most common subtype (62.6%), followed by triple-negative breast cancer (TNBC) at 16.4%, HER2-enriched (non-luminal) at 10.7%, and luminal A-like at 10.2%. Notably, the number of triple-positive tumors exceeded that of TNBC (23.38% vs. 16.4%). ER and PR positivity rates were 68.8% and 65.1%, respectively, and HER2 positivity was observed in 34.1% of cases. Invasive carcinoma of no special type constituted 95.5% of cases, with Grade 2 tumors being the most common (63.8%). CONCLUSION: The distribution of menopausal status, molecular subtypes, and histologic features in our cohort closely resembled patterns reported in Western populations. These findings emphasize the heterogeneity of patients and the need for generating local data to effectively address breast cancer treatment. CI - Copyright (c) 2026 Journal of Cancer Research and Therapeutics. FAU - Sulakshana, M S AU - Sulakshana MS AD - Department of Histopathology, Sri Shankara Cancer Hospital and Research Centre, Bangalore, Karnataka, India. FAU - Sreelakshmi, S AU - Sreelakshmi S AD - Department of Histopathology, Sri Shankara Cancer Hospital and Research Centre, Bangalore, Karnataka, India. FAU - Krishna, Kanyadhara Lohita AU - Krishna KL AD - Department of Surgical Oncology, Sri Shankara Cancer Hospital and Research Centre, Bangalore, Karnataka, India. FAU - Velusamy, Shanthi AU - Velusamy S AD - Department of Histopathology, Sri Shankara Cancer Hospital and Research Centre, Bangalore, Karnataka, India. FAU - Divya, V AU - Divya V AD - Department of Histopathology, Sri Shankara Cancer Hospital and Research Centre, Bangalore, Karnataka, India. FAU - Chaitra, V AU - Chaitra V AD - Department of Histopathology, Sri Shankara Cancer Hospital and Research Centre, Bangalore, Karnataka, India. FAU - Kumari, Prasanna AU - Kumari P AD - Department of Molecular Oncology, Sri Shankara Cancer Hospital and Research Centre, Bangalore, Karnataka, India. FAU - Srinath, B S AU - Srinath BS AD - Department of Surgical Oncology, Sri Shankara Cancer Hospital and Research Centre, Bangalore, Karnataka, India. FAU - Kumar, Rekha V AU - Kumar RV AD - Department of Histopathology, Sri Shankara Cancer Hospital and Research Centre, Bangalore, Karnataka, India. LA - eng PT - Journal Article DEP - 20261029 PL - India TA - J Cancer Res Ther JT - Journal of cancer research and therapeutics JID - 101249598 RN - EC 2.7.10.1 (Erb-b2 Receptor Tyrosine Kinases) RN - 0 (Biomarkers, Tumor) RN - 0 (Receptors, Estrogen) RN - 0 (Receptors, Progesterone) RN - EC 2.7.10.1 (ERBB2 protein, human) SB - IM MH - Humans MH - Female MH - India/epidemiology MH - Tertiary Care Centers/statistics & numerical data MH - Middle Aged MH - Erb-b2 Receptor Tyrosine Kinases/metabolism MH - Retrospective Studies MH - *Biomarkers, Tumor/metabolism MH - *Breast Neoplasms/pathology/epidemiology/metabolism MH - Receptors, Estrogen/metabolism MH - Receptors, Progesterone/metabolism MH - Adult MH - Aged MH - Immunohistochemistry MH - *Triple Negative Breast Neoplasms/pathology/epidemiology/metabolism MH - In Situ Hybridization, Fluorescence OTO - NOTNLM OT - Breast cancer OT - luminal B breast cancer OT - molecular subtypes OT - triple-positive breast cancer EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:35 PHST- 2025/09/29 00:00 [received] PHST- 2026/06/01 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:35 [entrez] AID - 01363817-202610000-00017 [pii] AID - 10.4103/jcrt.jcrt_2072_25 [doi] PST - ppublish SO - J Cancer Res Ther. 2026 Jul 1;22(5):903-910. doi: 10.4103/jcrt.jcrt_2072_25. Epub 2026 Oct 29. PMID- 42955968 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1998-4138 (Electronic) IS - 1998-4138 (Linking) VI - 22 IP - 5 DP - 2026 Jul 1 TI - From classical survival statistics to explainable AI: A multi-method prognostic framework for triple negative breast cancer. PG - 869-883 LID - 10.4103/jcrt.jcrt_118_26 [doi] AB - Triple-negative breast cancer (TNBC) is a biologically heterogeneous subtype associated with variable survival outcomes, limiting the performance of conventional prognostic models. This study integrates classical survival analysis, machine-learning methods, and explainable artificial intelligence to identify key prognostic factors in a retrospective cohort of 869 histopathologically confirmed TNBC patients treated between 2018 and 2024. Kaplan-Meier analysis and Cox proportional hazards regression were used to evaluate survival differences and estimate hazard ratios, with assessment of proportional hazards assumptions using Schoenfeld residuals. Machine-learning models were developed for fixed-horizon survival prediction, and model interpretability was explored using SHAP and LIME analyses to characterize feature contributions. Chemotherapy was associated with improved overall survival in unadjusted analysis (log-rank P < 0.001), while radiotherapy showed no significant association after adjustment. Multivariable Cox analysis identified stage (HR =4.23, P < 0.001), absence of lymphovascular invasion (HR =0.15, P < 0.001), surgery (HR =0.77, P = 0.003), and chemotherapy-related variables as independent prognostic factors. Among machine-learning models, the artificial neural network showed the highest discrimination (C-index =0.98 +/- 0.01), followed by random forest (0.92 +/- 0.02). SHAP analyses consistently identified stage, tumor size, lymphovascular invasion, chemotherapy, and surgery as key contributors to model predictions. Overall, the combined framework demonstrates improved predictive performance and interpretability compared with traditional models; however, findings are based on internal validation and require external validation before clinical application. CI - Copyright (c) 2026 Journal of Cancer Research and Therapeutics. FAU - Padhi, Diana AU - Padhi D AD - Department of Radiation Oncology, All India Institute of Medical Sciences, Bhubaneswar, Odisha, India. FAU - Majumdar, Saroj Kumar Das AU - Majumdar SKD LA - eng PT - Journal Article DEP - 20261029 PL - India TA - J Cancer Res Ther JT - Journal of cancer research and therapeutics JID - 101249598 SB - IM MH - Humans MH - *Triple Negative Breast Neoplasms/mortality/therapy/pathology MH - Female MH - Prognosis MH - *Artificial Intelligence MH - Retrospective Studies MH - Middle Aged MH - Predictive Learning Models MH - Proportional Hazards Models MH - *Machine Learning MH - Kaplan-Meier Estimate MH - Random Forest MH - Adult MH - Aged OTO - NOTNLM OT - Artificial intelligence OT - Cox proportional hazards regression OT - LIME OT - SHAP OT - Triple-negative breast cancer OT - survival prediction EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:35 PHST- 2026/01/20 00:00 [received] PHST- 2026/08/23 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:35 [entrez] AID - 01363817-202610000-00014 [pii] AID - 10.4103/jcrt.jcrt_118_26 [doi] PST - ppublish SO - J Cancer Res Ther. 2026 Jul 1;22(5):869-883. doi: 10.4103/jcrt.jcrt_118_26. Epub 2026 Oct 29. PMID- 42955961 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1998-4138 (Electronic) IS - 1998-4138 (Linking) VI - 22 IP - 5 DP - 2026 Jul 1 TI - Depth of invasion and early recurrence among patients with recurrent oral cavity squamous cell carcinoma after surgery. PG - 819-825 LID - 10.4103/jcrt.jcrt_2236_25 [doi] AB - BACKGROUND: Early recurrence after curative surgery for oral cavity squamous cell carcinoma (OSCC) is associated with poor outcomes, yet factors influencing the timing of recurrence are not well defined. This study evaluated determinants of early versus late recurrence among patients with recurrent OSCC, focusing on the predictive utility of depth of invasion (DOI). METHODS: We retrospectively analyzed 52 patients with OSCC who developed recurrence after curative-intent surgery. Recurrences were classified as early (6 months) based on disease-free interval. Clinical, pathological, and treatment-related variables were assessed. Univariate and multivariable logistic regression were used to explore factors associated with early recurrence. Receiver operating characteristic (ROC) analysis was performed to evaluate the discriminatory ability of DOI and to identify an optimal cut-off. RESULTS: Of 52 patients, 11 (21.2%) experienced early recurrence and 41 (78.8%) had late recurrence. In multivariable analysis, greater DOI was significantly associated with early recurrence (adjusted odds ratio 3.73; 95% confidence interval 1.20-11.60; P = 0.023). ROC analysis demonstrated discriminatory performance for DOI (area under the curve 0.80). A DOI threshold of 0.6 cm achieved 100% sensitivity and 51.7% specificity for distinguishing early from late recurrence; all early recurrences occurred in patients with DOI >/= 0.6 cm. CONCLUSION: In this recurrent OSCC cohort, greater DOI was associated with early recurrence, and a DOI >/= 0.6 cm identified all early relapse cases at the cost of modest specificity. These hypothesis-generating findings suggest that DOI may help inform risk-adapted early postoperative surveillance but require validation in larger, prospective studies. CI - Copyright (c) 2026 Journal of Cancer Research and Therapeutics. FAU - Dwivedi, Surjeet AU - Dwivedi S AD - Department of Surgery, AFMC, Pune, Maharashtra, India. FAU - Sanjay, Wg Cdr AU - Sanjay WC AD - Department of ENT, AFCME, New Delhi, India. FAU - Kumari, Sabita AU - Kumari S AD - Eye Spl, Manipal Hospital, Pune, Maharashtra, India. FAU - Thakur, Nikhil AU - Thakur N AD - Department of MDTC, Command Hospital, Bengaluru, Karnataka, India. FAU - Hans, Raj AU - Hans R AD - Department of MDTC, Command Hospital, Kolkata, West Bengal, India. FAU - Gautam, Siddarth AU - Gautam S AD - Department of Surgery, AFMC, Pune, Maharashtra, India. FAU - Chowdhary, Shreyansh AU - Chowdhary S AD - Department of Surgery, 05 Air Force Hospital, Jorhat, Assam, India. FAU - Jyoti, Kshitij AU - Jyoti K AD - Department of Surgery, AFMC, Pune, Maharashtra, India. FAU - Kumar, Manoj AU - Kumar M AD - Department of ENT, Base Hospital, Delhi Cantt, New Delhi, India. LA - eng PT - Journal Article DEP - 20261029 PL - India TA - J Cancer Res Ther JT - Journal of cancer research and therapeutics JID - 101249598 SB - IM MH - Humans MH - *Mouth Neoplasms/pathology/surgery MH - *Neoplasm Recurrence, Local/pathology/epidemiology MH - Female MH - Male MH - Middle Aged MH - Retrospective Studies MH - Neoplasm Invasiveness MH - ROC Curve MH - Aged MH - Prognosis MH - *Carcinoma, Squamous Cell/surgery/pathology MH - Neoplasm Staging MH - Adult OTO - NOTNLM OT - Depth of invasion OT - disease-free interval OT - early recurrence OT - head and neck squamous cell carcinoma OT - oral cavity squamous cell carcinoma OT - postoperative surveillance OT - prognostic factors EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:35 PHST- 2025/11/02 00:00 [received] PHST- 2026/02/11 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:35 [entrez] AID - 01363817-202610000-00007 [pii] AID - 10.4103/jcrt.jcrt_2236_25 [doi] PST - ppublish SO - J Cancer Res Ther. 2026 Jul 1;22(5):819-825. doi: 10.4103/jcrt.jcrt_2236_25. Epub 2026 Oct 29. PMID- 42955957 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1998-4138 (Electronic) IS - 1998-4138 (Linking) VI - 22 IP - 5 DP - 2026 Jul 1 TI - When toxicity limits cure: Repositioning radiation in esophageal and rectal cancers by proton therapy. PG - 791-794 LID - 10.4103/jcrt.jcrt_121_26 [doi] AB - For locally advanced esophageal and rectal cancers, radiotherapy (RT) provides essential local control but is frequently limited by acute and late toxicities that erode survival and quality of life (QoL). Over the last decade, evidence has implicated radiation dose to the heart, lungs, bowel, and pelvic bone marrow in increased postoperative morbidity, cardiopulmonary deaths, and hematologic toxicity, compromising chemotherapy delivery and long-term outcomes. The physical properties of proton beam therapy (PBT), which eliminate exit dose and reduce integral dose, offer a fundamental advantage. Systematic evidence confirms PBT consistently lowers low- to intermediate-dose exposure to organs at risk (OARs). For esophageal cancer, robust dosimetric data show reductions of approximately 50-65% in mean heart dose and 40-50% in lung V5, translating to reduced total toxicity burden (TTB) and perioperative complications in randomized and real-world studies. In rectal cancer, PBT reduces pelvic bone marrow V10 by 40-55% and small bowel V15-V30 by 30-50%, a prerequisite for improving the tolerability of intensive regimens. While definitive Phase III survival data are awaited, current evidence supports the selective use of PBT to improve the therapeutic index of radiotherapy, allowing its benefits to be realized with less collateral damage. CI - Copyright (c) 2026 Journal of Cancer Research and Therapeutics. FAU - Abhishek, Ashu AU - Abhishek A AD - Department of Radiation Oncology, Apollo Proton Cancer Center, Chennai, Tamil Nadu, India. FAU - Reddy, Anupamma AU - Reddy A FAU - Nangia, Sapna AU - Nangia S FAU - Chilukuri, Srinivas AU - Chilukuri S FAU - Jalali, Rakesh AU - Jalali R LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - India TA - J Cancer Res Ther JT - Journal of cancer research and therapeutics JID - 101249598 SB - IM MH - Humans MH - *Proton Therapy/adverse effects/methods MH - *Rectal Neoplasms/radiotherapy/pathology MH - *Esophageal Neoplasms/radiotherapy/pathology MH - Organs at Risk/radiation effects MH - *Radiation Injuries/etiology/prevention & control MH - Radiotherapy Dosage MH - Quality of Life OTO - NOTNLM OT - Esophageal cancer OT - proton therapy OT - radiation toxicity OT - rectal cancer EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:35 PHST- 2026/01/20 00:00 [received] PHST- 2026/08/06 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:35 [entrez] AID - 01363817-202610000-00003 [pii] AID - 10.4103/jcrt.jcrt_121_26 [doi] PST - ppublish SO - J Cancer Res Ther. 2026 Jul 1;22(5):791-794. doi: 10.4103/jcrt.jcrt_121_26. Epub 2026 Oct 29. PMID- 42955945 OWN - NLM STAT- Publisher LR - 20261029 IS - 2211-1247 (Electronic) VI - 45 IP - 11 DP - 2026 Oct 29 TI - ALS-linked FUS mutation impairs GEMIN5-dependent mRNA recruitment to neuronal late endosomes. PG - 118206 LID - S2211-1247(26)01285-4 [pii] LID - 10.1016/j.celrep.2026.118206 [doi] AB - Neurons depend on tightly regulated positioning of cellular components to maintain long-distance signaling, but the mechanisms guiding specific mRNAs to distant regions remain unclear. Here, we show that late endosomes function as selective RNA carriers in human motor neurons and uncover the molecular logic guiding their loading. Using APEX2-mediated proximity labeling, we identify the external transcriptome of RAB7A-positive endosomes and find a specific population of mRNAs enriched for endosomal, axonal, and synaptic functions. We find that mRNAs enriched in RAB7A endosomes contain evolutionarily conserved 5'UTRs that act as localization signals, and that the RNA-binding protein GEMIN5 interacts with these regions to promote endosomal RNA recruitment. Finally, we show that there is a conspicuous loss of endosome-associated transcripts in amyotrophic lateral sclerosis-associated conditions, accompanied by the mislocalization of GEMIN5 from endosomes. These findings uncover fundamental principles of RNA compartmentalization and highlight endosomal mRNA loading as a vulnerable axis in neuronal homeostasis. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Setti, Adriano AU - Setti A AD - Department of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00185 Rome, Italy. FAU - Stufera Mecarelli, Lorenzo AU - Stufera Mecarelli L AD - Center for Human Technologies, Fondazione Istituto Italiano di Tecnologia (IIT), 16152 Genoa, Italy. FAU - Santini, Tiziana AU - Santini T AD - Department of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00185 Rome, Italy. FAU - D'Angelo, Angelo AU - D'Angelo A AD - Department of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00185 Rome, Italy. FAU - Padovano, Vittorio AU - Padovano V AD - Department of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00185 Rome, Italy. FAU - Vitiello, Erika AU - Vitiello E AD - Institut fur Pharmazie und Molekulare Biotechnologie, Universitat Heidelberg, 69102 Heidelberg, Germany. FAU - Castagnetti, Francesco AU - Castagnetti F AD - Karlsruher Institute fur Technologie (KIT), 76131 Karlsruhe, Germany. FAU - Martone, Julie AU - Martone J AD - Institute of Molecular Biology and Pathology, National Research Council of Italy, 00185 Rome, Italy. FAU - Mariani, Davide AU - Mariani D AD - Department of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00185 Rome, Italy; Center for Human Technologies, Fondazione Istituto Italiano di Tecnologia (IIT), 16152 Genoa, Italy; Center for Life Nano- & Neuro-Science@Sapienza, Fondazione Istituto Italiano di Tecnologia (IIT), 00161 Rome, Italy. Electronic address: davide.mariani@uniroma1.it. FAU - Bozzoni, Irene AU - Bozzoni I AD - Department of Biology and Biotechnology "Charles Darwin", Sapienza University of Rome, 00185 Rome, Italy; Center for Human Technologies, Fondazione Istituto Italiano di Tecnologia (IIT), 16152 Genoa, Italy; Center for Life Nano- & Neuro-Science@Sapienza, Fondazione Istituto Italiano di Tecnologia (IIT), 00161 Rome, Italy. Electronic address: irene.bozzoni@uniroma1.it. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Cell Rep JT - Cell reports JID - 101573691 SB - IM OTO - NOTNLM OT - 5'UTR OT - ALS OT - APEX2 OT - CP: molecular biology OT - CP: neuroscience OT - FUS OT - GEMIN5 OT - RAB7A OT - RNA compartmentalization OT - late endosomes OT - proximity labeling COIS- Declaration of interests The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 13:35 PHST- 2026/02/26 00:00 [received] PHST- 2026/08/10 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:35 [entrez] AID - S2211-1247(26)01285-4 [pii] AID - 10.1016/j.celrep.2026.118206 [doi] PST - aheadofprint SO - Cell Rep. 2026 Oct 29;45(11):118206. doi: 10.1016/j.celrep.2026.118206. PMID- 42955931 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Study on the association between the low-density lipoprotein to high-density lipoprotein ratio and clinicopathological characteristics of breast cancer: A retrospective analysis incorporating subgroup evaluations by age, body mass index, and menopausal status. PG - e0361764 LID - 10.1371/journal.pone.0361764 [doi] AB - Breast cancer (BC) represents the most prevalent malignant tumor among women globally, with its pronounced heterogeneity presenting significant challenges for precision diagnosis and treatment. Aberrant lipid metabolism, particularly the ratio of low-density lipoprotein to high-density lipoprotein (LHR), has garnered considerable interest in relation to BC development and progression. Nevertheless, the association between LHR and clinicopathological characteristics remains inadequately explored. To address this gap, the present retrospective study analyzed 583 BC patients who underwent surgical treatment at Qilu Hospital of Shandong University. Preoperative blood lipid profiles and clinicopathological data were systematically collected to investigate the association between LHR and critical features, including molecular subtypes. The findings indicated that LHR levels were significantly elevated in patients aged 46 years or older, postmenopausal women, and individuals with a body mass index of 25 kg/m2 or higher. Subgroup analyses incorporating age, menopausal status, and BMI suggested potential interactions between these clinical factors and LHR. Although univariate analysis demonstrated an association between LHR and certain clinicopathological features, multivariate logistic regression analysis revealed that menopausal status, rather than LHR, was the sole independent predictor of ER-positive and PR-positive status. Therefore, LHR should not be considered an independent predictive biomarker for receptor status. In conclusion, although the LHR demonstrates statistically significant associations with certain clinicopathological characteristics, its modest effect sizes and discriminative ability close to chance indicate limited clinical utility as an independent biomarker. These preliminary findings highlight the need for future research focused on identifying reliable metabolic signatures. Such studies should consider utilizing multi-marker panels instead of relying exclusively on individual lipid metrics for risk stratification in breast cancer. CI - Copyright: (c) 2026 Dong et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Dong, Lei AU - Dong L AUID- ORCID: 0009-0000-8370-3788 AD - Department of Breast Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, China. FAU - Liu, Can AU - Liu C AD - Department of Thyroid and Breast Surgery, Jinan Third People's Hospital, Jinan, Shandong, China. FAU - Li, Lixin AU - Li L AD - Department of Breast Surgery, Jinan Maternity Child Careand Hospital, Jinan, Shandong, China. FAU - Zhang, Xiaotong AU - Zhang X AD - Minimally invasive surgery, First People's Hospital of Jinan, Jinan, Shandong, China. FAU - Xu, Wei AU - Xu W AD - Department of Plastic Surgery, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, Qingdao, Shandong, China. FAU - Dai, Gaosai AU - Dai G AD - Department of Breast Surgery, Qilu Hospital of Shandong University, Jinan, Shandong, China. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (Lipoproteins, LDL) RN - 0 (Lipoproteins, HDL) SB - IM MH - Humans MH - Female MH - Middle Aged MH - *Breast Neoplasms/pathology/blood/metabolism MH - Retrospective Studies MH - Body Mass Index MH - *Menopause MH - *Lipoproteins, LDL/blood MH - *Lipoproteins, HDL/blood MH - Aged MH - Age Factors MH - Adult COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:34 PHST- 2025/12/06 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:34 [entrez] AID - PONE-D-25-63389 [pii] AID - 10.1371/journal.pone.0361764 [doi] PST - epublish SO - PLoS One. 2026 Oct 29;21(10):e0361764. doi: 10.1371/journal.pone.0361764. eCollection 2026. PMID- 42955924 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Vertical accuracy of ASTER, SRTM, and NASADEM digital surface models across Malawi's diverse topography. PG - e0361397 LID - 10.1371/journal.pone.0361397 [doi] AB - Digital Surface Models (DSMs) are widely used as important sources of topographic information for earth science and environmental applications. The accuracy of DSMs has direct impacts on several environmental calculations and processes. Therefore, it is imperative to select the most suitable DSM based on the aim and accuracy requirements of each application. However, the use of an appropriate DSM has always been a challenge. This study evaluated the vertical accuracy and performance of the freely available Shuttle Radar Topography Mission (SRTM), NASA DEM (NASADEM), and Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER). The DSM elevations were measured against high-precision national geodetic control points. Among other accuracy metrics, we computed Root Mean Square Error (RMSE) and examined the influence of elevation, slope, aspect, curvature, and land cover. At the national scale, SRTM (RMSE of 19.55 m) and NASADEM (RMSE of 19.99 m) showed comparable performance, while ASTER produced a higher RMSE of 22.13 m. However, the principal finding was that DSM accuracy varied substantially across Malawi's diverse terrain. All the DSMs performed particularly well in the low-lying Lower Shire Valley, with RMSEs of 3.25 m (NASADEM), 4.30 m (SRTM), and 6.91 m (ASTER). Errors increased markedly in the Highlands, where RMSE reached 35.03 m for NASADEM, 33.62 m for SRTM, and 36.94 m for ASTER. In addition, accuracy decreased with increasing elevation and slope, particularly in highland regions and on slopes steeper than 30 degrees . Aspect showed the highest relative importance in the Random Forest analysis. However, the differences in accuracy among aspect classes were comparatively modest, and greater underestimation was observed on north- and west-facing slopes. These findings demonstrate that national-scale accuracy statistics alone do not adequately describe DSM suitability across Malawi's diverse landscapes. DSM selection should therefore consider the terrain and land-cover characteristics of the intended application area. CI - Copyright: (c) 2026 Nazombe et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Nazombe, Kennedy Sones AU - Nazombe KS AUID- ORCID: 0000-0001-5800-1872 AD - Department of Land and Water Resources, Faculty of Life Sciences and Natural Resources, Lilongwe University of Agriculture and Natural Resources, Lilongwe, Malawi. FAU - Chunga, Brighton A AU - Chunga BA AUID- ORCID: 0000-0002-5681-1537 AD - Department of Water and Sanitation, Faculty of Environmental Sciences, Mzuzu University, Mzuzu, Malawi. AD - Institute for Sustainability and Corporate Citizenship, University of South Africa, Pretoria, South Africa. AD - Stockholm Environment Institute, World Agroforestry Centre, United Nations Avenue, Gigiri, Nairobi, Kenya. FAU - Bakolo, Charles AU - Bakolo C AD - Department of Surveys, Ministry of Lands, Housing and Urban Development, Lilongwe, Malawi. FAU - Mlewa, Rodrick AU - Mlewa R AD - Department of Land and Water Resources, Faculty of Life Sciences and Natural Resources, Lilongwe University of Agriculture and Natural Resources, Lilongwe, Malawi. FAU - Nhamo, Godwell AU - Nhamo G AD - Institute for Sustainability and Corporate Citizenship, University of South Africa, Pretoria, South Africa. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Malawi MH - *Models, Theoretical COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:33 PHST- 2026/01/12 00:00 [received] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:33 [entrez] AID - PONE-D-26-00523 [pii] AID - 10.1371/journal.pone.0361397 [doi] PST - epublish SO - PLoS One. 2026 Oct 29;21(10):e0361397. doi: 10.1371/journal.pone.0361397. eCollection 2026. PMID- 42955915 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2767-3375 (Electronic) IS - 2767-3375 (Linking) VI - 6 IP - 10 DP - 2026 TI - Bacterial culture positivity in placental basal plate tissue and associated factors among women with spontaneous preterm births at Mubende Regional Referral Hospital, Uganda: A cross-sectional study. PG - e0007002 LID - 10.1371/journal.pgph.0007002 [doi] AB - Aerobic bacterial culture positivity in placental basal plate tissue and its associated maternal factors remain poorly described in Uganda. This study estimated the prevalence of culture positivity and identified associated factors among women with spontaneous preterm births at Mubende Regional Referral Hospital. We conducted a hospital-based analytical cross-sectional study among 383 women between 15 May and 15 September 2025. Placental basal plate tissue specimens were collected aseptically immediately after delivery and processed for aerobic bacterial culture. Sociodemographic, obstetric and clinical data were obtained using interviewer-administered questionnaires and medical record review. Associations were examined using multivariable logistic regression and reported as adjusted odds ratios (aORs) with 95% confidence intervals (CIs). Statistical significance was set at p < 0.05. Positive cultures were identified in 159 women, giving a prevalence of 41.5% (95% CI: 36.5-46.6). Culture positivity was associated with fever (aOR = 11.32, 95% CI: 4.89-26.19), abdominal pain or uterine tenderness (aOR = 9.13, 95% CI: 4.37-19.08), membrane rupture lasting more than 24 hours (aOR = 5.93, 95% CI: 2.08-16.89), HIV infection (aOR = 4.23, 95% CI: 1.21-14.74), urinary tract infection during pregnancy (aOR = 4.20, 95% CI: 2.13-8.27), foul-smelling vaginal discharge (aOR = 3.22, 95% CI: 1.48-6.97), traditional herb use (aOR = 2.19, 95% CI: 1.16-4.13) and no formal education (aOR = 6.97, 95% CI: 1.50-32.46). Earlier gestational age, prolonged labour and primary education were also associated with culture positivity. Positive aerobic placental basal plate cultures were common in this population. These findings support improved recognition and management of maternal infection during antenatal and intrapartum care. However, culture positivity alone cannot distinguish invasive infection from colonization or contamination, and prospective studies are needed to clarify temporal and causal relationships. CI - Copyright: (c) 2026 Kasiita et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Kasiita, Shem AU - Kasiita S AD - Department of Obstetrics and Gynecology, Kampala International University, Ishaka, Uganda. FAU - Ishimwe, Marie Pascaline Sabine AU - Ishimwe MPS AD - Department of Pediatrics and Child Health, Kampala International University, Ishaka, Uganda. FAU - Kasujja, Musa AU - Kasujja M AD - Department of Obstetrics and Gynecology, Kampala International University, Ishaka, Uganda. FAU - Nteziyaremye, Theodore AU - Nteziyaremye T AD - Department of Sciences, University of Rwanda, Kigali, Rwanda. FAU - Liban, Sara AU - Liban S AD - Department of Obstetrics and Gynecology, Kampala International University, Ishaka, Uganda. FAU - Racheal, Bivako AU - Racheal B AD - Department of Obstetrics and Gynecology, Kampala International University, Ishaka, Uganda. FAU - Kakande, Ronald AU - Kakande R AD - Department of Obstetrics and Gynecology, Kampala International University, Ishaka, Uganda. FAU - Bwire, Innocent AU - Bwire I AD - Department of Obstetrics and Gynecology, Kampala International University, Ishaka, Uganda. FAU - Neel, G R AU - Neel GR AD - Department of Microbiology, Kampala International University, Ishaka, Uganda. FAU - Namutebi, Sharifa AU - Namutebi S AD - Department of Obstetrics and Gynecology, Kampala International University, Ishaka, Uganda. FAU - Abdallah, Ziraba AU - Abdallah Z AUID- ORCID: 0009-0006-5534-6780 AD - Department of Foundations, Kampala International University, Ishaka, Uganda. FAU - Hakizimana, Theoneste AU - Hakizimana T AD - Department of Obstetrics and Gynecology, Kampala International University, Ishaka, Uganda. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - PLOS Glob Public Health JT - PLOS global public health JID - 9918283779606676 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:33 PHST- 2026/07/19 00:00 [received] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:33 [entrez] AID - PGPH-D-26-02794 [pii] AID - 10.1371/journal.pgph.0007002 [doi] PST - epublish SO - PLOS Glob Public Health. 2026 Oct 29;6(10):e0007002. doi: 10.1371/journal.pgph.0007002. eCollection 2026. PMID- 42955890 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1941-5923 (Electronic) IS - 1941-5923 (Linking) VI - 27 DP - 2026 Oct 29 TI - Tracheoscopic Management of Postoperative High Tracheoesophageal Fistula Using Covered Self-Expanding Metallic Stents: A Comparative Report of 2 Cases With Divergent Outcomes. PG - e953942 LID - 10.12659/AJCR.953942 [doi] AB - BACKGROUND Tracheoesophageal fistula (TEF) following esophageal cancer resection is a rare but life-threatening complication. Management of high postoperative TEF, particularly in the subglottic region, presents significant technical challenges due to anatomical constraints and the risk of stent migration. This report evaluates the efficacy of tracheoscopic placement of covered self-expanding metallic stents (CSEMS) for treating high postoperative TEF. CASE REPORT We report 2 cases of high TEF complicating esophageal cancer surgery. Case 1: A 67-year-old man with a 0.6 cm diameter fistula located 4 cm distal to the vocal folds. A straight CSEMS was successfully deployed. Four months later, after confirmation of complete mucosal healing and epithelialization, the stent was removed following the observation of granulation tissue hyperplasia. Case 2: A 78-year-old man with diabetes presented with a large (12x8 mm) fistula situated 3 cm distal to the vocal folds. An initial straight CSEMS failed due to repeated displacement. It was replaced with a customized ball-ended CSEMS and supplemented with biogel and -gelatin sponges. Due to refractory displacement and impaired healing, external percutaneous fixation was ultimately used to stabilize the stent. CONCLUSIONS Tracheoscopic CSEMS placement represents a viable management strategy for high postoperative TEF. However, successful outcomes in the challenging subglottic region are highly dependent on individualized stent selection and adjunctive measures, as illustrated by the contrasting courses of our 2 cases. These experiences underscore the importance of technical adaptability, while larger studies are needed to define optimal management algorithms. FAU - Pang, Xiaodong AU - Pang X AUID- ORCID: 0009-0007-3601-5377 AD - Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. FAU - Chen, Yili AU - Chen Y AD - Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. FAU - Liu, Yue AU - Liu Y AD - Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. FAU - Zeng, Yuanyuan AU - Zeng Y AD - Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. FAU - Gu, Lei AU - Gu L AD - Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. FAU - Huang, Jian-An AU - Huang JA AD - Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. FAU - Mu, Chuanyong AU - Mu C AD - Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. LA - eng PT - Case Reports PT - Comparative Study PT - Journal Article DEP - 20261029 PL - United States TA - Am J Case Rep JT - The American journal of case reports JID - 101489566 SB - IM MH - Humans MH - Male MH - Aged MH - *Tracheoesophageal Fistula/surgery/etiology MH - *Self Expandable Metallic Stents MH - *Esophageal Neoplasms/surgery MH - *Postoperative Complications/surgery MH - *Esophagectomy/adverse effects EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:23 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:23 [entrez] AID - 953942 [pii] AID - 10.12659/AJCR.953942 [doi] PST - epublish SO - Am J Case Rep. 2026 Oct 29;27:e953942. doi: 10.12659/AJCR.953942. PMID- 42955888 OWN - NLM STAT- In-Process LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Retraction: Aesthetic preference for artificially selected color variant affects mate choice copying behavior in female Poecilia latipinna. PG - e0361670 LID - 10.1371/journal.pone.0361670 [doi] CN - PLOS One Editors LA - eng PT - Journal Article PT - Retraction Notice DEP - 20261029 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM ROF - PLoS One. 2024 Mar 28;19(3):e0298171. doi: 10.1371/journal.pone.0298171. PMID: 38547203 EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 13:23 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:23 [entrez] AID - PONE-D-26-51679 [pii] AID - 10.1371/journal.pone.0361670 [doi] PST - epublish SO - PLoS One. 2026 Oct 29;21(10):e0361670. doi: 10.1371/journal.pone.0361670. eCollection 2026. PMID- 42955877 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2561-326X (Electronic) IS - 2561-326X (Linking) VI - 10 DP - 2026 Oct 29 TI - Discharge Mobility After Osteoporotic Thoracolumbar Fractures Using a 2-Stage Machine Learning Prediction Model: Prospective Cohort Study. PG - e100486 LID - 10.2196/100486 [doi] AB - BACKGROUND: Discharge mobility after osteoporotic vertebral fractures is heterogeneous and difficult to predict at admission. Machine learning offers structured approaches for multiclass functional outcome prediction, but existing models predominantly address binary end points and report global performance metrics without explicit analysis of the error structure. OBJECTIVE: This study aimed to develop and evaluate a 2-stage machine learning framework with a predefined decision policy designed to reduce extreme (LOW-HIGH) misclassification for graded discharge mobility prediction in hospitalized patients with osteoporotic thoracolumbar fractures. METHODS: This prospective, single-center cohort study enrolled consecutive patients aged 65 years or older admitted for acute osteoporotic thoracic or lumbar fractures (N=158). Discharge mobility was categorized as LOW (<50 m or nonambulatory), MID (50-500 m), or HIGH (>500 m or stair climbing). Clinical associations were analyzed using proportional odds ordinal regression. A predefined 2-stage architecture combined gradient-boosted trees (stage A: HIGH vs non-HIGH) and a random forest classifier (stage L: LOW vs non-LOW). The final 3-class prediction was assigned by a predefined decision policy designed to reduce clinically relevant extreme (LOW-HIGH) misclassification. All preprocessing steps, including fold-wise multiple imputation, were performed within training partitions only. The decision policy was additionally reevaluated in a nested cross-validation with fold-internal threshold selection across 10 repeated partitions. RESULTS: Prefracture HIGH mobility was the only significant independent predictor of discharge mobility (odds ratio [OR] 3.38, 95% CI 1.68-6.80; P<.001); age, comorbidity burden, fracture grade, and treatment were not. In the prespecified analysis with fixed thresholds, performance was moderate (accuracy 0.494; balanced accuracy 0.450; macro-F(1) 0.444). Binary discrimination yielded an area under the curve of 0.693 (HIGH vs non-HIGH) and 0.625 (LOW vs non-LOW). Using the predefined decision policy, major LOW-HIGH errors were less frequent than with the prefracture mobility-only baseline (5 vs 22), and no HIGH-to-LOW misclassification occurred in the prespecified analysis. In the leakage-free nested cross-validation, macro-F(1) was 0.388 (SD 0.031) and major errors had a mean of 8.3 (SD 3.4) vs 22.5 (SD 1.1) for the baseline, with fewer major errors in all 10 partitions. HIGH-to-LOW errors had a mean of 2.6 (SD 2.2) in this analysis. CONCLUSIONS: Discharge mobility after osteoporotic thoracolumbar fractures is primarily determined by prefracture functional reserve rather than fracture severity or comorbidity. A 2-stage machine learning framework achieved moderate multiclass discrimination and reduced extreme LOW-HIGH misclassification relative to a mobility-only baseline; this reduction persisted under nested cross-validation, whereas the absence of HIGH-to-LOW errors was specific to the prespecified partition. External validation is required before clinical deployment. CI - (c) Anna Voelker, Dina Wilma Wiersbicki, Christoph-Eckhard Heyde, Tim Stelzner, Erik Schreiber, Thomas Neumuth, Juliane Neumann. Originally published in JMIR Formative Research (https://formative.jmir.org). FAU - Voelker, Anna AU - Voelker A AUID- ORCID: 0000-0002-2715-5438 AD - Department for Orthopedics, Trauma and Plastic Surgery, University Hospital Leipzig, Liebigstrasse 20, Leipzig, Saxony, 04103, Germany, 49 03419723000, 49 03419723009. FAU - Wiersbicki, Dina Wilma AU - Wiersbicki DW AUID- ORCID: 0009-0004-1156-6162 AD - Department for Orthopedics, Trauma and Plastic Surgery, University Hospital Leipzig, Liebigstrasse 20, Leipzig, Saxony, 04103, Germany, 49 03419723000, 49 03419723009. FAU - Heyde, Christoph-Eckhard AU - Heyde CE AUID- ORCID: 0000-0002-9083-4062 AD - Department for Orthopedics, Trauma and Plastic Surgery, University Hospital Leipzig, Liebigstrasse 20, Leipzig, Saxony, 04103, Germany, 49 03419723000, 49 03419723009. FAU - Stelzner, Tim AU - Stelzner T AUID- ORCID: 0009-0006-4145-4713 AD - Innovation Center Computer Assisted Surgery (ICCAS), Medizinische Fakultat, Leipzig University, Leipzig, Saxony, Germany. FAU - Schreiber, Erik AU - Schreiber E AUID- ORCID: 0000-0002-5500-8080 AD - Innovation Center Computer Assisted Surgery (ICCAS), Medizinische Fakultat, Leipzig University, Leipzig, Saxony, Germany. FAU - Neumuth, Thomas AU - Neumuth T AUID- ORCID: 0000-0001-6999-5024 AD - Innovation Center Computer Assisted Surgery (ICCAS), Medizinische Fakultat, Leipzig University, Leipzig, Saxony, Germany. FAU - Neumann, Juliane AU - Neumann J AUID- ORCID: 0000-0002-5696-9473 AD - Innovation Center Computer Assisted Surgery (ICCAS), Medizinische Fakultat, Leipzig University, Leipzig, Saxony, Germany. LA - eng PT - Journal Article DEP - 20261029 PL - Canada TA - JMIR Form Res JT - JMIR formative research JID - 101726394 SB - IM MH - Humans MH - Prospective Studies MH - Female MH - Predictive Learning Models MH - *Machine Learning/standards MH - Aged MH - Male MH - *Osteoporotic Fractures MH - *Patient Discharge/statistics & numerical data/standards MH - Aged, 80 and over MH - *Spinal Fractures MH - Cohort Studies MH - Lumbar Vertebrae/injuries MH - Classification Algorithms MH - Prediction Algorithms MH - Thoracic Vertebrae/injuries MH - Boosting Machine Learning Algorithms OTO - NOTNLM OT - clinical prediction rules OT - functional outcome OT - geriatric assessment OT - machine learning OT - mobility limitation OT - osteoporotic fractures OT - patient discharge OT - risk assessment OT - spine OT - vertebral fractures EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 13:13 PHST- 2026/05/06 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:13 [entrez] AID - v10i1e100486 [pii] AID - 10.2196/100486 [doi] PST - epublish SO - JMIR Form Res. 2026 Oct 29;10:e100486. doi: 10.2196/100486. PMID- 42955876 OWN - NLM STAT- Publisher LR - 20261029 IS - 1673-5374 (Print) IS - 1673-5374 (Linking) DP - 2026 Oct 29 TI - Single-cell and spatial transcriptomics reveal mesenchymal stem cell-associated neuroimmune and neuronal signaling patterns after ischemic stroke. LID - 10.4103/NRR.NRRONLINE-D-26-00871 [doi] AB - Ischemic stroke induces coordinated yet spatially heterogeneous neuroimmune and neuronal responses that shape secondary injury and tissue remodeling. However, how intranasal mesenchymal stem cell administration is associated with these cell type-specific responses remains incompletely understood. Here, we integrated single-cell RNA sequencing of the ipsilateral cerebrum on days 1, 7, and 14 after middle cerebral artery occlusion/reperfusion with spatial transcriptomic profiling on day 7. A total of 55,520 high-quality single-cell transcriptomes were obtained from control, middle cerebral artery occlusion/reperfusion, and mesenchymal stem cell-treated samples. In this exploratory dataset, the middle cerebral artery occlusion/reperfusion samples showed relatively greater representation of inflammatory and antigen-presenting microglial states and elevated expression of selected microglial state-associated genes, including Slfn4, Apoe, and Gpnmb. In the corresponding exploratory comparison, the mesenchymal stem cell-treated sample showed relatively greater representation of homeostatic-associated and Lgals3/Spp1-enriched microglial states and lower expression of several inflammatory-associated genes. Spatial mapping further revealed regionally restricted expression of microglial and neuronal markers within the injured tissue. Excitatory neurons displayed preliminary mesenchymal stem cell-associated transcriptional patterns involving mitochondrial transcripts, oxidative phosphorylation, synaptic signaling, calcium-related functions, and apoptosis/autophagy-associated pathways. CellChat analysis computationally predicted relatively high levels of tumor necrosis factor, C-X-C motif chemokine ligand, and complement signaling in the middle cerebral artery occlusion/reperfusion sample, whereas the mesenchymal stem cell-treated sample had relatively high levels of predicted activity of neuregulin, pleiotrophin and selected semaphorin. Together, these integrated datasets provide a hypothesis-generating framework linking microglial state remodeling, neuronal metabolic and synaptic programs, and predicted neuroimmune communication patterns after ischemic stroke. Because each omics condition or time point was represented by one biological sample, all the transcriptomic findings should be considered preliminary and exploratory and should be validated in independently replicated cohorts. CI - Copyright (c) 2026 Neural Regeneration Research. FAU - Wu, Qinfen AU - Wu Q AD - Department of Neurology, The Second Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, China. FAU - Hu, Junhua AU - Hu J AD - Department of Neurosurgery, The 990th Hospital of Joint Logistics Support Force, Zhumadian, Henan Province, China. FAU - Abuliezi, Naibijiang AU - Abuliezi N AD - Department of Neurology, The Second Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, China. FAU - Sun, Yihan AU - Sun Y AD - Department of Neurology, The Second Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, China. FAU - Wu, Yanyan AU - Wu Y AD - Department of Neurology, The Second Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, China. FAU - Dun, Xiaoyi AU - Dun X AUID- ORCID: 0009-0003-0682-9768 AD - Department of Hematology, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region, China. LA - eng PT - Journal Article DEP - 20261029 PL - India TA - Neural Regen Res JT - Neural regeneration research JID - 101316351 OTO - NOTNLM OT - cell-cell communication OT - ischemic stroke OT - mesenchymal stem cells OT - microglia OT - middle cerebral artery occlusion OT - neuroinflammation OT - single-cell RNA sequencing OT - spatial transcriptomics EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 13:13 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 13:13 [entrez] AID - 01300535-990000000-01408 [pii] AID - 10.4103/NRR.NRRONLINE-D-26-00871 [doi] PST - aheadofprint SO - Neural Regen Res. 2026 Oct 29. doi: 10.4103/NRR.NRRONLINE-D-26-00871. PMID- 42955872 OWN - NLM STAT- Publisher LR - 20261029 IS - 1557-900X (Electronic) IS - 0892-7790 (Linking) DP - 2026 Oct 29 TI - Impact of Three-Dimensional Reconstruction on Partial Nephrectomy Outcomes: A Systematic Review and Meta-Analysis. PG - 8927790261502938 LID - 10.1177/08927790261502938 [doi] AB - INTRODUCTION: Three-dimensional (3D) reconstruction may improve anatomical planning in partial nephrectomy, but its clinical impact remains unclear. This study aims to evaluate the impact of 3D reconstruction on perioperative outcomes in partial nephrectomy. METHODS: A systematic review and meta-analysis were conducted following PRISMA 2020 (PROSPERO CRD420261373519). PubMed/MEDLINE, EMBASE, Cochrane, and Web of Science were searched. Randomized and nonrandomized studies comparing 3D reconstruction with two-dimensional imaging in adults undergoing partial nephrectomy were included. Pooled estimates were expressed as mean differences (MDs) or risks ratio (RR) with 95% CIs. Subgroup analyses considered tumor complexity, study design, 3D technology, and surgical approach. RESULTS: Twenty-eight studies (3196 patients) were included. 3D reconstruction reduced warm ischemia time (MD -1.31 minutes; p = 0.02), operative time (MD -8.30 minutes; p = 0.0002), and estimated blood loss (MD -18.95 mL; p = 0.006). It also lowered overall complications (RR 0.74; p = 0.006) and global ischemia (RR 0.72; p = 0.005), with higher selective clamping rates (RR 1.96; p = 0.0006). Smaller declines in eGFR (MD -2.02; p = 0.0006), shorter hospital stay (MD -0.32; p /=13 years who underwent CTPA for suspected PE. Following the decision to order CTPA, the modified Wells and simplified revised Geneva scores were calculated, and the YEARS and PEGeD strategies were applied. Clinically significant PE, defined as segmental or more proximal embolism, was the primary outcome; any PE, including isolated subsegmental embolism, was the secondary outcome. Among 408 patients, clinically significant PE was confirmed in 144 (35.3%) and any PE in 154 (37.7%). The Wells score demonstrated better discrimination for clinically significant PE than the simplified revised Geneva score (area under the curve 0.725 [95%CI: 0.671-0.779] vs 0.656 [95%CI: 0.598-0.714]; P = 0.0003). Clinically significant PE was present in 21.6% and 24.4% of patients classified as PE unlikely by the Wells and simplified revised Geneva two-level classifications, respectively. Among 398 patients with D-dimer results, YEARS and PEGeD each demonstrated 96.5% sensitivity but identified only 13.8% and 13.6% of patients, respectively, as potentially eligible for imaging avoidance. Diagnostic performance showed a similar pattern for any PE. In this CTPA-selected cohort, the Wells score demonstrated better discrimination than the simplified revised Geneva score, but both two-level classifications yielded substantial counterfactual missed-PE rates. YEARS and PEGeD maintained high sensitivity but offered limited theoretical imaging-sparing potential. CI - (c) 2026. The Author(s), under exclusive licence to Societa Italiana di Medicina Interna (SIMI). FAU - Pannu, Ashok Kumar AU - Pannu AK AUID- ORCID: 0000-0002-4476-3478 AD - Department of Internal Medicine, Postgraduate Institute of Medical Education and Research, Nehru Hospital, 4thFloor, F Block, Sector 12, Chandigarh, 160012, India. gawaribacchi@gmail.com. FAU - Saroch, Atul AU - Saroch A AUID- ORCID: 0000-0001-9723-6500 AD - Department of Internal Medicine, Postgraduate Institute of Medical Education and Research, Nehru Hospital, 4thFloor, F Block, Sector 12, Chandigarh, 160012, India. atulsaroch@gmail.com. FAU - Bhatia, Vikas AU - Bhatia V AUID- ORCID: 0000-0003-3700-8025 AD - Department of Radiodiagnosis, Postgraduate Institute of Medical Education and Research, Nehru Hospital, Sector 12, Chandigarh, 160012, India. FAU - Ahluwalia, Jasmina AU - Ahluwalia J AUID- ORCID: 0000-0002-0949-3476 AD - Department of Hematology, Postgraduate Institute of Medical Education and Research, Research Block A, Sector 12, Chandigarh, 160012, India. FAU - Sharma, Navneet AU - Sharma N AUID- ORCID: 0000-0001-5707-9686 AD - Department of Internal Medicine, Postgraduate Institute of Medical Education and Research, Nehru Hospital, 4thFloor, F Block, Sector 12, Chandigarh, 160012, India. LA - eng PT - Journal Article DEP - 20261028 PL - Italy TA - Intern Emerg Med JT - Internal and emergency medicine JID - 101263418 SB - IM OTO - NOTNLM OT - Computed tomography pulmonary angiography OT - Emergency department OT - Pulmonary embolism OT - Simplified revised Geneva score OT - Wells score OT - YEARS strategy COIS- Declarations. Conflict of interest: No authors have any relevant conflict of interest disclosures. This manuscript in part or in full has not been submitted or published anywhere. Ethical approval: The authors state that they have obtained appropriate institutional review board approval from the Institutional Ethics Committee, Postgraduate Institute of Medical Education and Research, Chandigarh, India (Approval No.: INT/IEC/2019/001605, dated 13 August 2019). Written informed consent was obtained from all participants or their legally authorized representatives, in accordance with the Declaration of Helsinki. Associated data. The manuscript has no associated data. Writing disclosure. No writing assistance was utilized in the production of this manuscript. Standard language-editing software may have been used for minor grammar and readability improvements. AI-based tools and technologies used in content generation or proofing. No AI-based tools and technologies were used in content generation or proofing. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:34 PHST- 2026/05/27 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:34 [entrez] AID - 10.1007/s11739-026-04573-x [pii] AID - 10.1007/s11739-026-04573-x [doi] PST - aheadofprint SO - Intern Emerg Med. 2026 Oct 28. doi: 10.1007/s11739-026-04573-x. PMID- 42955691 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 3079 DP - 2027 TI - Tutorial: End-to-End Deep Learning Pipeline for RNA-RNA Interaction Prediction. PG - 329-343 LID - 10.1007/978-1-0716-5567-2_24 [doi] AB - Bridging methodological advances and practical applications in computational biology requires clear, reproducible tutorials. This chapter provides an end-to-end guide for constructing deep learning pipelines for RNA-RNA interaction (RRI) prediction. Starting from raw RNA sequences, it covers dataset curation, negative sampling, feature engineering, model selection, training, evaluation, and interpretability using explainable AI techniques. Multiple modeling strategies are illustrated, including sequence-based Transformers for capturing long-range dependencies and structure-aware Graph Neural Networks (GNNs) that encode secondary structures and topological relationships. Hybrid architectures integrating sequence and structural information are also demonstrated to improve predictive performance on complex RNAs. Emphasis is placed on reproducibility, with recommendations for workflow management, containerization, version-controlled datasets, and FAIR-compliant pipelines to facilitate sharing and reuse. Transparent discussion of design choices, preprocessing, and evaluation enables readers to develop robust, interpretable, and transferable RRI prediction models. This chapter equips students, researchers, and practitioners with practical tools to apply advanced deep learning to real-world RNA biology problems. CI - (c) 2027. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Harithanush, S AU - Harithanush S AUID- ORCID: 0009-0007-2338-988X AD - Department of Computer Science, SASTRA University, Thanjavur, India. sharithanush@gmail.com. FAU - Chokalingam, Punitha AU - Chokalingam P AD - Department of Computer Science, SASTRA University, Thanjavur, India. FAU - Varadarajan, Swetha AU - Varadarajan S AD - Department of Computer Science, SASTRA University, Thanjavur, India. LA - eng PT - Journal Article PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 RN - 63231-63-0 (RNA) SB - IM MH - *RNA/chemistry/metabolism/genetics MH - *Deep Learning MH - *Computational Biology/methods MH - Software MH - Graph Neural Networks MH - Sequence Analysis, RNA/methods OTO - NOTNLM OT - Deep learning OT - FAIR workflows OT - Graph Neural Networks OT - RNA-RNA interactions OT - Reproducibility OT - Transformers OT - Tutorial COIS- Conflict of Interest. The authors have no conflicts of interest to declare that are relevant to the content of this chapter. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:34 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:34 [entrez] AID - 10.1007/978-1-0716-5567-2_24 [doi] PST - ppublish SO - Methods Mol Biol. 2027;3079:329-343. doi: 10.1007/978-1-0716-5567-2_24. PMID- 42955681 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 3079 DP - 2027 TI - Genetic Algorithm-Based Solvers for RNA-RNA Interaction. PG - 201-216 LID - 10.1007/978-1-0716-5567-2_14 [doi] AB - RNA-RNA interactions (RRIs) are fundamental to a wide range of cellular processes, including gene regulation, mRNA stabilization, and viral replication. Computational prediction of RRIs has traditionally relied on dynamic programming-based approaches grounded in thermodynamic models, which, although accurate, are computationally intensive and face scalability limitations when applied to large or complex RNA sequences. To address these challenges, recent research has explored alternative paradigms based on metaheuristic optimization, particularly Genetic Algorithms (GAs), which reformulate RRI prediction as an optimization problem and enable efficient exploration of large and complex search spaces. Existing studies demonstrate that GA-based methods encode RNA interaction patterns as candidate solutions and iteratively refine them using biologically inspired operations such as selection, crossover, and mutation, while integrating thermodynamic and structural constraints into fitness functions. Comparative analyses in the literature indicate that these approaches can achieve competitive accuracy with traditional methods while offering improved flexibility and scalability for large-scale and heuristic-driven RRI prediction tasks. CI - (c) 2027. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. FAU - B, Jaganath AU - B J AUID- ORCID: 0009-0009-1358-2760 AD - Department of Computer Science, SASTRA University, Tirumalaisamudram, Thanjavur, India. jaganathbabu025@gmail.com. FAU - Ramanathan, Vignesh AU - Ramanathan V AUID- ORCID: 0009-0006-8519-9726 AD - Department of Computer Science, SASTRA University, Tirumalaisamudram, Thanjavur, India. FAU - Varadarajan, Swetha AU - Varadarajan S AUID- ORCID: 0000-0002-0256-7639 AD - Department of Computer Science, SASTRA University, Tirumalaisamudram, Thanjavur, India. LA - eng PT - Journal Article PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 RN - 63231-63-0 (RNA) SB - IM MH - *Computational Biology/methods MH - *RNA/chemistry/genetics/metabolism MH - Genetic Algorithms MH - Thermodynamics MH - Algorithms MH - Nucleic Acid Conformation OTO - NOTNLM OT - Bioinformatics OT - Evolutionary computing OT - Fitness function OT - Genetic algorithms OT - Heuristics OT - Non-coding RNA OT - Optimization OT - RNA structure prediction OT - RNA-RNA interaction OT - Sequence pairing COIS- Conflict of Interest. The authors have no conflicts of interest to declare that are relevant to the content of this chapter. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:34 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:34 [entrez] AID - 10.1007/978-1-0716-5567-2_14 [doi] PST - ppublish SO - Methods Mol Biol. 2027;3079:201-216. doi: 10.1007/978-1-0716-5567-2_14. PMID- 42955677 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 3079 DP - 2027 TI - Evaluating Web Servers for RNA-RNA Interaction. PG - 137-151 LID - 10.1007/978-1-0716-5567-2_10 [doi] AB - RNA-RNA interactions (RRIs) play a critical role in post-transcriptional regulation and functional dynamics of non-coding RNAs, influencing biological processes such as splicing, translation, and gene silencing. With the expansion of non-coding RNA discovery and interaction databases, there is an increasing need for computational tools that can accurately predict RRIs. Web servers serve as accessible platforms that integrate various predictive models and user interfaces for this purpose. This review presents a comprehensive survey of existing web servers designed for RRI prediction, focusing on the computational backbone of each tool. We categorize the reviewed tools based on their machine learning (ML) or deep learning (DL) frameworks, feature extraction pipelines, and sequence- or structure-based encoding strategies. We also compare thermodynamics-based methods with hybrid approaches that integrate biological knowledge with data-driven models. Special attention is given to how each server handles RNA secondary structures, including pseudoknots, and incorporates interaction features such as energy profiles, accessibility metrics, and evolutionary conservation. Furthermore, we evaluate the usability, availability, input formats, output interpretation, and dataset support for each web server. Tools are benchmarked using publicly available RRI datasets, with a discussion on their generalizability and limitations. By highlighting trends in feature engineering, encoding schemes, and algorithmic design, this review serves as a resource for researchers aiming to select or design RRI prediction tools suited to their biological question. CI - (c) 2027. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Verma, Purushottham Kumar AU - Verma PK AUID- ORCID: 0009-0002-5224-8983 AD - Department of Computer Science, SASTRA University, Thanjavur, Tirumalaisamudram, India. purushottamdav@gmail.com. FAU - Varadarajan, Swetha AU - Varadarajan S AD - Department of Computer Science, SASTRA University, Thanjavur, Tirumalaisamudram, India. LA - eng PT - Journal Article PT - Review PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 RN - 63231-63-0 (RNA) RN - 0 (RNA, Untranslated) SB - IM MH - *Computational Biology/methods MH - Internet MH - *RNA/chemistry/metabolism MH - *Software MH - Machine Learning MH - Nucleic Acid Conformation MH - *RNA, Untranslated/chemistry/metabolism/genetics MH - Thermodynamics MH - Humans OTO - NOTNLM OT - Bioinformatics tools OT - Data encoding OT - Deep learning OT - Feature engineering OT - Machine learning OT - Non-coding RNA OT - Pseudoknots OT - RNA interaction Prediction OT - RNA structure OT - RNA-RNA interactions (RRIs) OT - Secondary structure OT - Thermodynamics OT - Web servers COIS- Conflict of Interest. The authors have no conflicts of interest to declare that are relevant to the content of this chapter. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:34 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:34 [entrez] AID - 10.1007/978-1-0716-5567-2_10 [doi] PST - ppublish SO - Methods Mol Biol. 2027;3079:137-151. doi: 10.1007/978-1-0716-5567-2_10. PMID- 42955675 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 3079 DP - 2027 TI - Comparative Analysis of Hyperparameter Optimization Strategies for RNA-RNA Interaction Prediction. PG - 107-122 LID - 10.1007/978-1-0716-5567-2_8 [doi] AB - RNA-RNA interactions (RRIs) play a fundamental role in numerous biological processes, including gene regulation, viral replication, and RNA-based therapeutics. In recent years, deep learning approaches have emerged as powerful tools for predicting RRIs, offering improved performance over traditional computational methods. This study presents a comprehensive comparative analysis of hyperparameter optimization strategies in deep learning models for RNA-RNA interaction prediction. We evaluate prominent architectures, including Convolutional Neural Networks (CNNs), Long Short-Term Memory (LSTM) networks, hybrid LSTM-CNN models, attention-based Transformers, and Graph Neural Networks (GNNs), in conjunction with optimization techniques such as Genetic Algorithms, Bayesian Optimization, random search, and transfer learning. The comparison is conducted using benchmark RRI datasets, considering performance metrics such as precision, recall, F1-score, computational efficiency, and convergence behavior. Additionally, we analyze feature representation strategies, including sequence encoding, secondary structure integration, and thermodynamic properties, and examine their interaction with hyperparameter configurations. The results highlight the strengths and limitations of different optimization strategies across model architectures, providing insights into their impact on prediction accuracy, generalizability, and training efficiency. This study serves as a guide for selecting appropriate deep learning and hyperparameter optimization frameworks for RNA interactome analysis, paving the way for more scalable and robust RRI prediction systems. CI - (c) 2027. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Jaganath, B AU - Jaganath B AD - Department of Computer Science, SASTRA University, Thanjavur, Tirumalaisamudram, India. jaganathbabu025@gmail.com. FAU - Varadarajan, Swetha AU - Varadarajan S AD - Department of Computer Science, SASTRA University, Thanjavur, Tirumalaisamudram, India. LA - eng PT - Comparative Study PT - Journal Article PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 RN - 63231-63-0 (RNA) SB - IM MH - *RNA/chemistry/metabolism/genetics MH - *Computational Biology/methods MH - Long Short Term Memory MH - Algorithms MH - Deep Learning MH - Convolutional Neural Networks MH - Bayes Theorem MH - Prediction Algorithms MH - Graph Neural Networks MH - Humans MH - Genetic Algorithms OTO - NOTNLM OT - Bayesian optimization OT - Computational biology OT - Convolutional neural networks OT - Deep learning OT - Feature representation OT - Genetic algorithms OT - Graph neural networks OT - Hyperparameter optimization OT - Long short-term memory OT - RNA-RNA interactions OT - Transformers COIS- Conflict of Interest. The authors have no conflicts of interest to declare that are relevant to the content of this chapter. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:34 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:34 [entrez] AID - 10.1007/978-1-0716-5567-2_8 [doi] PST - ppublish SO - Methods Mol Biol. 2027;3079:107-122. doi: 10.1007/978-1-0716-5567-2_8. PMID- 42955672 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 3079 DP - 2027 TI - Feature Set Analysis of RNA-RNA Interaction Dataset. PG - 61-72 LID - 10.1007/978-1-0716-5567-2_5 [doi] AB - Understanding RNA-RNA interactions (RRIs) is fundamental to unraveling complex biological processes, including gene regulation, RNA splicing, translation, localization, and degradation. In this work, we present a comprehensive machine learning-based feature set analysis of RNA-RNA interaction datasets to identify features that contribute most significantly to accurate interaction prediction. By leveraging diverse machine learning (ML) methods, including feature selection algorithms and explainable AI tools, we systematically analyze multiple feature categories, such as sequence composition, secondary structure, thermodynamic properties, and base-pairing probabilities. Comparative experiments using classifiers such as Random Forests (RF), Support Vector Machines (SVM), Multi-Layer Perceptrons (MLP), and Convolutional Neural Networks (CNNs) demonstrate that optimized feature sets improve predictive performance while reducing computational overhead. Feature importance analysis highlights biologically relevant signals, including thermodynamic stability, conserved motifs, and structural flexibility, which are critical for RNA interaction prediction. Furthermore, the integration of SHAP (SHapley Additive Explanations) and LIME (Local Interpretable Model-agnostic Explanations) enhances model interpretability, allowing alignment of computational predictions with biological reasoning. This work provides a thorough methodological and biological framework for RRI prediction, supporting downstream applications such as RNA-targeted therapeutics, functional annotation of non-coding RNAs, and transcriptome-wide interaction mapping. The presented pipeline serves as a robust foundation for developing interpretable, efficient, and biologically meaningful RNA-RNA interaction prediction tools. CI - (c) 2027. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Ramanathan, Vignesh AU - Ramanathan V AUID- ORCID: 0009-0006-8519-9726 AD - Computer Science Department, SASTRA University, Tirumalaisamudram, Thanjavur, 613401, India. vigneshramanathan3@gmail.com. FAU - Varadarajan, Swetha AU - Varadarajan S AD - Computer Science Department, SASTRA University, Tirumalaisamudram, Thanjavur, 613401, India. LA - eng PT - Journal Article PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 RN - 63231-63-0 (RNA) SB - IM MH - *RNA/chemistry/metabolism/genetics MH - *Computational Biology/methods MH - Support Vector Machine MH - Thermodynamics MH - Machine Learning MH - Nucleic Acid Conformation MH - Algorithms MH - Random Forest MH - Prediction Algorithms MH - Convolutional Neural Networks OTO - NOTNLM OT - Base-pairing probabilities OT - Computational biology OT - Deep learning OT - Feature selection OT - Feature set analysis OT - Machine learning OT - Model explainability OT - Predictive performance OT - RNA datasets OT - RNA-RNA interactions OT - Random forests OT - Secondary structure OT - Sequence composition OT - Support vector machines OT - Thermodynamic properties COIS- Conflict of Interest. The authors have no conflicts of interest to declare that are relevant to the content of this chapter. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:34 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:34 [entrez] AID - 10.1007/978-1-0716-5567-2_5 [doi] PST - ppublish SO - Methods Mol Biol. 2027;3079:61-72. doi: 10.1007/978-1-0716-5567-2_5. PMID- 42955666 OWN - NLM STAT- Publisher LR - 20261029 IS - 1179-1950 (Electronic) IS - 0012-6667 (Linking) DP - 2026 Oct 29 TI - Comparative Effects of Pharmacological Treatment on Heart Failure with Mildly Reduced or Preserved Ejection Fraction: A Systematic Review and Network Meta-analysis of Randomized Controlled Trials. LID - 10.1007/s40265-026-02409-8 [doi] AB - BACKGROUND: The comparative efficacy and safety of the new drugs (including sodium-glucose cotransporter-2 inhibitors [SGLT-2Is], glucagon-like peptide-1 receptor agonists [GLP-1RAs], non-steroidal mineralocorticoid receptor antagonists [nsMRAs], and soluble guanylate cyclase stimulators [sGCs]) for heart failure with mildly reduced or preserved (HFmrEF/HFpEF) ejection fraction remains largely unclear. We conducted a systematic review and network meta-analysis to evaluate their comparative effects on HFmrEF/HFpEF, aiming to provide evidence-based guidance for treatment selection. METHODS: MEDLINE, EMBASE, Web of Science, and Cochrane Central Register of Controlled Trials were comprehensively searched. We included randomized controlled trials that compared nsMRA, sGC, SGLT-2I, and GLP-1RA with placebo for patients with HFmrEF/HFpEF. Primary efficacy outcomes were cardiovascular (CV) death, hospitalization for heart failure (HF hospitalization), and all-cause mortality, while primary safety outcomes were adverse events (AEs) and serious adverse events (SAEs). The main analysis was conducted to compare the effects on HFmrEF/HFpEF between individual drugs. A frequentist network meta-analysis with random-effects models was used to calculate risk ratios (RRs) for dichotomous outcomes and standardized mean differences (SMDs) for continuous outcomes. RESULTS: A total of 20 randomized controlled trials (RCTs, including 10 drugs from 35 publications) were included for analyses, but none provided head-to-head direct comparisons. No significant differences among all the individual drugs were found in risks of CV death or all-cause mortality. Compared with exenatide, low or moderate certainty evidence showed that dapagliflozin (RR 0.57, 95% CI 0.36-0.91), empagliflozin (RR 0.54, 95% CI 0.34-0.86), and semaglutide (RR 0.43, 95% CI 0.20-0.91) were related to decreased risk of HF hospitalization. No significant differences in the risk of SAEs were found among the individual drugs. Compared with empagliflozin, very low certainty evidence showed that tirzepatide (RR 1.17, 95% CI 1.04-1.32) was related to increased risk of AEs. When comparing the four drug classes (nsMRA, sGC, SGLT-2I, and GLP-1RA), no significant differences in efficacy outcomes were found, while SGLT-2I (RR 0.87, 95% CI 0.78-0.96) was associated with a decreased risk of AEs compared with GLP-1RA. CONCLUSIONS: No significant differences among the individual SGLT-2I, GLP-1RA, nsMRA, and sGC drugs were found in risks of CV death, all-cause mortality or SAEs in patients with HFmrEF/HFpEF. Further evidence is needed to support these comparative effects and update guidance on medication choice for HFmrEF/HFpEF. REGISTRATION: PROSPERO identifier number CRD420251143542. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature Switzerland AG. FAU - Zhang, Jingyi AU - Zhang J AD - Center for Clinical Epidemiology and Methodology (CCEM), The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou, Guangdong, China. FAU - Wang, Yongjian AU - Wang Y AD - School of Public Health, The Second Clinical Medical School of Guangdong Pharmaceutical University (Guangdong Second Provincial General Hospital), Guangzhou, Guangdong, China. FAU - Li, Jianfeng AU - Li J AD - School of Public Health, The Second Clinical Medical School of Guangdong Pharmaceutical University (Guangdong Second Provincial General Hospital), Guangzhou, Guangdong, China. FAU - Chen, Yujiong AU - Chen Y AD - School of Public Health, The Second Clinical Medical School of Guangdong Pharmaceutical University (Guangdong Second Provincial General Hospital), Guangzhou, Guangdong, China. FAU - Peng, Xinru AU - Peng X AD - School of Public Health, The Second Clinical Medical School of Guangdong Pharmaceutical University (Guangdong Second Provincial General Hospital), Guangzhou, Guangdong, China. FAU - Thabane, Lehana AU - Thabane L AD - Department of Health Research Methods, Evidence, and Impact (HEI), McMaster University, Hamilton, ON, Canada. AD - Father Sean O'Sullivan Research Centre, St Joseph's Healthcare Hamilton, Hamilton, ON, Canada. FAU - Lip, Gregory Y H AU - Lip GYH AD - Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, United Kingdom. AD - Department of Clinical Medicine, Aalborg University, Aalborg, Denmark. AD - Department of Cardiology, Lipidology and Internal Medicine with Intensive Coronary Care Unit, Medical University of Bialystok, Bialystok, Poland. FAU - Li, Guowei AU - Li G AD - Center for Clinical Epidemiology and Methodology (CCEM), The Affiliated Guangdong Second Provincial General Hospital of Jinan University, Guangzhou, Guangdong, China. ligw@gd2h.org.cn. AD - School of Public Health, The Second Clinical Medical School of Guangdong Pharmaceutical University (Guangdong Second Provincial General Hospital), Guangzhou, Guangdong, China. ligw@gd2h.org.cn. AD - Father Sean O'Sullivan Research Centre, St Joseph's Healthcare Hamilton, Hamilton, ON, Canada. ligw@gd2h.org.cn. LA - eng GR - 82473612/the National Natural Science Foundation of China/ GR - 2025A1515010779/the Natural Science Foundation of Guangdong Province of China/ GR - YY2018-002/the Science Foundation of Guangdong Second Provincial General Hospital/ GR - TJGC-2025006/the Science Foundation of Guangdong Second Provincial General Hospital/ GR - 0720240244/the Young Top Talent Project in the Special Support Plan for Training High-level Talents in Guangdong/ PT - Journal Article PT - Systematic Review DEP - 20261029 PL - New Zealand TA - Drugs JT - Drugs JID - 7600076 SB - IM COIS- Declarations. Conflict of Interest: Jingyi Zhang, Yongjian Wang, Jianfeng Li, Yujiong Chen, Xinru Peng, Lehana Thabane, Gregory Y.H. Lip, and Guowei Li declare that they have no potential conflicts of interest that might be relevant to the contents of this manuscript. Data Availability: Not applicable because this study was based exclusively on published literature. Ethical Approval: Not applicable. Code Availability: Not applicable. Consent to Participate: Not applicable. Consent for Publication: Not applicable. AI use: No AI tools were used for this article. Author Contributions: JZ and GL conceived the idea of the study and designed the study. JZ, YW, JL, YC, XP, and GL contributed to record screening and data collection. JZ and GL performed statistical analyses and interpretation, and drafted the manuscript. GYHL and LT provided professional support and made several critical revisions to the manuscript. All authors read and approved the final manuscript. GL acts as the guarantor of this work. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:33 PHST- 2026/05/14 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:33 [entrez] AID - 10.1007/s40265-026-02409-8 [pii] AID - 10.1007/s40265-026-02409-8 [doi] PST - aheadofprint SO - Drugs. 2026 Oct 29. doi: 10.1007/s40265-026-02409-8. PMID- 42955665 OWN - NLM STAT- Publisher LR - 20261029 IS - 1179-1950 (Electronic) IS - 0012-6667 (Linking) DP - 2026 Oct 28 TI - Twelve-Month Cumulative Incidence, Characteristics, and Correlates of Non-adherence in Youths Treated with Second-Generation Antipsychotics for Mental Disorders: Results from a Prospective Cohort Study. LID - 10.1007/s40265-026-02403-0 [doi] AB - BACKGROUND: Antipsychotic non-adherence in youths with mental disorders is multifactorial, may remain clinically unnoticed, and has been underresearched. OBJECTIVE: To characterize antipsychotic non-adherence in youths, including its correlates. METHOD: Data were collected from the naturalistic Second-Generation Antipsychotic Treatment Indications, Effectiveness and Tolerability in Youth (SATIETY) cohort study, which included youths (ages 4-17 years) who initiated clinician's choice second-generation antipsychotics (SGAs) for psychosis-, mood-, or disruptive behavior-spectrum disorders and had >/= 1 outpatient day of follow-up. Non-adherence was assessed via interview and antipsychotic plasma levels, and cumulative rates were analyzed via Kaplan-Meyer survival analysis and observed incidences. Characteristics of non-adherence and non-adherent patients were examined. Three multivariable regression models were used to identify predictors of observed cumulative non-adherence over 12 months. RESULTS: Altogether, 521 youths (median age 14.9 years; male 55.1%; post-pubertal 72.7%; antipsychotic-naive 65.5%; inpatients 74.7%; mood-spectrum disorder 56.5%, schizophrenia-spectrum disorder 22.9%, aggression-spectrum disorder 20.6%) started risperidone (37.2%), aripiprazole (26.9%), quetiapine (22.8%), or olanzapine (13.1%). The observed 12-month cumulative SGA non-adherence (occurring after a median follow-up of 75 days, interquartile range [IQR] 30-135 days) was 24.4% (overt 61.4%, covert/blood level-detected 38.6%; decision by: patient 75.6%, caregiver 18.1%, both 6.3%), increasing to 36.2% (95% confidence interval [CI] 30.4-41.9%) in the survival analysis. Non-mutually exclusive reasons for non-adherence, defined as SGA discontinuation against medical advice, included perceived lack of SGA treatment need (87.4%), intolerability (22.1%), and/or inefficacy (4.7%). In the first multivariable model in the full dataset, non-adherence was predicted by substance use disorder (odds ratio [OR] 3.38; 95% CI 1.85-6.17), non-white origin (OR 1.62; 95% CI 1.04-2.52), more last visit psychotropic medications (OR 1.60; 95% CI 1.27-2.00), and shorter pre-baseline lifetime antipsychotic treatment (OR 1.09; 95% CI 1.00-1.13 per month) (n = 515, r(2) = 0.10, p < 0.0001). Including non-dual-biological parent household (available in n = 435) in the second, exploratory model, non-dual-biological parent household and the four predictors of the first model predicted non-adherence (r(2) = 0.13, p < 0.0001). Finally, including last visit patient and caregiver Drug Attitude Inventory (DAI)-30/DAI-10 ratings (available in n = 273) to the third, exploratory model, substance use disorder, non-dual-biological parent household, more last visit psychiatric medications, worse last visit DAI-30 patient ratings, and risperidone treatment predicted non-adherence (r(2) = 0.17, p < 0.0001). CONCLUSIONS: One in four youths starting SGAs (increasing in survival analyses accounting for early dropouts to more than one in three youth) discontinued SGAs against medical advice within 12 months, mainly owing to perceived lack of treatment need. As much as 39% of the non-adherence would have remained undetected without SGA plasma level assessments. Interventions should target patient, caregiver, and medication factors to reduce barriers and improve outcomes. CI - (c) 2026. The Author(s). FAU - Roehrich, Lea A AU - Roehrich LA AD - Department of Child and Adolescent Psychiatry, Charite Universitaetsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany. FAU - Solmi, Marco AU - Solmi M AD - Department of Child and Adolescent Psychiatry, Charite Universitaetsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany. AD - Department of Psychiatry, University of Ottawa, Ottawa, ON, Canada. AD - Department of Mental Health, The Ottawa Hospital, Ottawa, ON, Canada. AD - Ottawa Hospital Research Institute (OHRI), Ottawa, ON, Canada. AD - School of Epidemiology and Public Health, Faculty of Medicine, University of Ottawa, Ottawa, Canada. FAU - Cortese, Samuele AU - Cortese S AD - Developmental EPI (Evidence synthesis, Prediction, Implementation) Lab, Centre for Innovation in Mental Health, School of Psychology, Faculty of Environmental and Life Sciences, University of Southampton, Southampton, UK. AD - Clinical and Experimental Sciences (CNS and Psychiatry), Faculty of Medicine, University of Southampton, Southampton, UK. AD - Hampshire and Isle of Wight Healthcare NHS Foundation Trust, Southampton, UK. AD - Hassenfeld Children's Hospital at NYU Langone, New York University Child Study Center, New York, NY, USA. AD - DiMePRe-J-Department of Precision and Regenerative Medicine-Jonic Area, University of Bari "Aldo Moro", Bari, Italy. FAU - Hojlund, Mikkel AU - Hojlund M AD - Department of Psychiatry Aabenraa, Mental Health Services Region of Southern Denmark, Aabenraa, Denmark. AD - Clinical Pharmacology, Pharmacy, and Environmental Medicine, Department of Public Health, University of Southern Denmark, Odense, Denmark. AD - Child and Adolescent Mental Health Centre, Mental Health Services Capital Region of Denmark, Copenhagen, Denmark. FAU - Salazar-de-Pablo, Gonzalo AU - Salazar-de-Pablo G AD - Department of Child and Adolescent Psychiatry, Institute of Psychiatry, Psychology and Neuroscience, King's College London, London, UK. AD - Child and Adolescent Mental Health Services, South London and Maudsley NHS Foundation Trust, London, UK. AD - Department of Child and Adolescent Psychiatry, Institute of Psychiatry and Mental Health, Hospital General Universitario Gregorio Maranon School of Medicine, Universidad Complutense, IiSGM, CIBERSAM, Madrid, Spain. FAU - Uhlhaas, Peter J AU - Uhlhaas PJ AD - Department of Child and Adolescent Psychiatry, Charite Universitaetsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany. FAU - Correll, Christoph U AU - Correll CU AUID- ORCID: 0000-0002-7254-5646 AD - Department of Child and Adolescent Psychiatry, Charite Universitaetsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany. christoph.correll@charite.de. AD - Departments of Psychiatry and Molecular Medicine, Donald and Barbara Zucker School of Medicine at Hostra/Northwell, Hempstead, USA. christoph.correll@charite.de. AD - Department of Psychiatry, The Zucker Hillside Hospital, Northwell Health, Glen Oaks, NY, USA. christoph.correll@charite.de. AD - Center for Psychiatric Neuroscience, The Feinstein Institute for Medical Research, Northwell Health, New Hyde Park, NY, USA. christoph.correll@charite.de. AD - German Center for Mental Health (DZPG), Partner Site Berlin, Berlin, Germany. christoph.correll@charite.de. AD - German Center for Child and Adolescent Health (DZKJ), Partner Site Berlin, Berlin, Germany. christoph.correll@charite.de. AD - Einstein Center for Population Diversity (ECPD), Berlin, Germany. christoph.correll@charite.de. LA - eng GR - FIMR-0092/Feinstein Institutes for Medical Research/ GR - P30MH090590/Advanced Center for Intervention and Services Research for the Study of/ PT - Journal Article DEP - 20261028 PL - New Zealand TA - Drugs JT - Drugs JID - 7600076 SB - IM COIS- Declarations. Conflicts of interest: M. Solmi, MD, PhD received honoraria/has been a consultant for Angelini, AbbVie, Bausch Health, Boehringer Ingelheim, Lundbeck, Otsuka, Teva, and is co-founder of MESAS srl. S. Cortese has declared reimbursement for travel and accommodation expenses from the Association for Child and Adolescent Central Health (ACAMH) in relation to lectures delivered for ACAMH, the Canadian ADHD Alliance Resource, the British Association of Psychopharmacology, Healthcare Convention and CCM Group team for educational activity on ADHD, and has received honoraria from Medice. M. Hojlund has been a consultant and/or received honoraria from: Lundbeck and Otsuka. G. Salazar de Pablo has received honoraria from Janssen-Cilag, Lundbeck and Angelini. P. Uhlhaas, PhD, has received honoraria/has been a consultant for Angelini and Boehringer Ingelheim. C. U. Correll has been a consultant and/or advisor to or has received honoraria from: AbbVie, Alkermes, Allergan, Angelini, Aristo, Autobahn, Boehringer-Ingelheim, Bristol-Meyers Squibb, Cardio Diagnostics, Cerevel, CNX Therapeutics, Compass Pathways, Darnitsa, Delpor, Denovo, Draig, Eli Lilly, Eumentis Therapeutics, Gedeon Richter, GH, Hikma, Holmusk, IntraCellular Therapies, Jamjoom Pharma, Janssen/J&J, Karuna, LB Pharma, Lundbeck, MedInCell, MedLink Global, Merck, Mindpax, Mitsubishi Tanabe Pharma, Maplight, Mylan, Neumora Therapeutics, Neuraxpharm, Neurocrine, Neurelis, Neurosterix, NeuShen, Neusignal Therapeutics, Newron, Noven, Novo Nordisk, Orion Pharma, Otsuka, PPD Biotech, Recognify Life Science, Recordati, Relmada, Response Pharmaeutical, Reviva, Rovi, Saladax, Sanofi, Seqirus, Servier, Sumitomo Pharma America, Sunovion, Sun Pharma, Supernus, Tabuk, Takeda, Teva, Terran, Tolmar, Vertex, Viatris and Xenon Pharmaceuticals. He provided expert testimony for Janssen, Lundbeck, Neurocrine, and Otsuka. He served on a Data Safety Monitoring Board for Compass Pathways, IntraCellular Therapies, Relmada, Reviva, Rovi. He has received grant support from Boehringer-Ingelheim, Janssen and Takeda. He received royalties from UpToDate and is also a stock option or stockholder of Cardio Diagnostics, Kuleon Biosciences, LB Pharma, MedLink Global, Mindpax, Quantic, Terran. C.U. Correll is an Editorial Board member of Drugs. CU Correll was not involved in the selection of peer reviewers for the manuscript nor any of the subsequent editorial decisions. L. A. Roehrich has no potential conflicts of interest to declare. Ethics approval: The SATIETY study was approved by the Northwell Health Institutional Review Board (01.04.137T). Consent to participate: Legal caregivers provided written informed consent, and minors (aged 9-17 years) provided written informed assent to participate in the study. Availability of data and material: The study data are not openly available as the consent to participate in the SATIETY study did not include consent to share the data outside of the study team. Consent for publication: Not applicable. Code availability: Not applicable. Authors' contribution: Conceptualization-study idea, aims, hypotheses: LAR, CUC. Methodology-study design and methods: All authors. Investigation-conducting the study/data acquisition: CUC. Resources-provision of patients, datasets, sites, materials, infrastructure: CUC. Data curation-data management, cleaning, preparation: LAR, CUC. Formal analysis-statistical analyses: LAR. Validation-verification/checking of analyses or results: LAR, CUC. Visualization-figures and tables: LAR. Writing-original draft-initial manuscript preparation: LAR, CUC. Writing-review and editing-substantive manuscript revision: All authors. Supervision-scientific/clinical oversight: CUC. Project administration-study coordination/management: CUC. Funding acquisition-obtaining study funding: CUC. All authors have read and approved the final version of the manuscript and agree to be accountable for all aspects of the work. AI use: No AI tools were used. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:33 PHST- 2026/01/11 00:00 [received] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:33 [entrez] AID - 10.1007/s40265-026-02403-0 [pii] AID - 10.1007/s40265-026-02403-0 [doi] PST - aheadofprint SO - Drugs. 2026 Oct 28. doi: 10.1007/s40265-026-02403-0. PMID- 42955664 OWN - NLM STAT- Publisher LR - 20261029 IS - 1556-2891 (Electronic) IS - 1547-769X (Linking) DP - 2026 Oct 29 TI - Beyond morphology: a combined histopathological and immunohistochemical protocol for the post-mortem diagnosis of the electrical mark. LID - 10.1007/s12024-026-01379-3 [doi] AB - The histopathological diagnosis of the electrical mark is hampered by the morphological overlap with thermal burns and abrasions and by the absence of standardized immunohistochemical criteria. This study aimed to develop and preliminarily evaluate an integrated histological and immunohistochemical protocol for the microscopic diagnosis of documented electrocution. Twenty-four full-thickness cutaneous samples from forensic cases with documented electrocution were stained with hematoxylin-eosin and processed for immunohistochemistry with Cytokeratin AE1/AE3. Six representative cases were also stained with HSP27, HSP70, and HSP90 antibodies. Histological findings were summarized as frequencies with exact 95% binomial confidence intervals (CIs). Ordinal immunohistochemical scores assigned independently by three observers were evaluated using a two-way random-effects, absolute-agreement intraclass correlation coefficient for single measurements [ICC(A,1)], pairwise weighted Cohen's kappa, and observed agreement. Epidermal and dermal coagulative necrosis, collagen homogenization, vascular dilatation/congestion, and hemorrhage/thrombosis were observed in 24/24 samples (100%; exact 95% CI 85.8-100%). Epidermal and dermal nuclear elongation and dark-stained nuclei occurred in 23/24 samples (95.8%; 95% CI 78.9-99.9%). For Cytokeratin, ICC(A,1) was 0.748 (95% CI 0.568-0.873); pairwise linearly weighted kappa values ranged from 0.437 to 0.820, and exact agreement among all three observers was 43.5%. HSP scores were identical among observers across the six selected slides (ICC and pairwise weighted kappa = 1.000; exact agreement 100%, 95% CI 54.1-100%). The combined assessment of H&E features and Cytokeratin/HSP immunoreactivity identifies a recurrent histopathological pattern that supports a provisional hierarchy of findings and can improve the standardization of microscopic assessment of electrical marks. CI - (c) 2026. The Author(s). FAU - Manta, Anna Maria AU - Manta AM AUID- ORCID: 0000-0002-3464-3560 AD - Department of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, Rome, Italy. annamaria.manta@uniroma1.it. FAU - Caruso, Anna Claudia AU - Caruso AC AD - Department of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, Rome, Italy. FAU - Morena, Donato AU - Morena D AD - Department of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, Rome, Italy. FAU - Marchesini, Damiano AU - Marchesini D AD - Department of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, Rome, Italy. FAU - Fabrello, Chiara AU - Fabrello C AD - Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy. FAU - Scopetti, Matteo AU - Scopetti M AD - Department of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, Rome, Italy. FAU - Cipolloni, Luigi AU - Cipolloni L AD - Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy. FAU - Fineschi, Vittorio AU - Fineschi V AD - Department of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, Rome, Italy. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Forensic Sci Med Pathol JT - Forensic science, medicine, and pathology JID - 101236111 SB - IM OTO - NOTNLM OT - AE1/AE3 OT - Cytokeratin OT - Electrical mark OT - Electrocution OT - Forensic pathology OT - HSP OT - Heat-shock protein OT - Immunohistochemistry COIS- Declarations. Ethics approval: This is a retrospective observational study on archived autoptic material. All procedures were conducted in accordance with the ethical standards of the institutional research committee and with the 1964 Declaration of Helsinki and its later amendments. Consent to participate / Consent to publish: The study was granted exemption from the requirement of informed consent, given the retrospective nature of the analysis and the full anonymization of the data. Use of generative AI: No generative AI tools were used for the generation of scientific content. AI-assisted copy editing was used solely for language polishing, in accordance with the journal's policy. Competing interests: The authors have no relevant financial or non-financial interests to disclose. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:33 PHST- 2026/06/17 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:33 [entrez] AID - 10.1007/s12024-026-01379-3 [pii] AID - 10.1007/s12024-026-01379-3 [doi] PST - aheadofprint SO - Forensic Sci Med Pathol. 2026 Oct 29. doi: 10.1007/s12024-026-01379-3. PMID- 42955659 OWN - NLM STAT- Publisher LR - 20261029 IS - 2509-2723 (Electronic) IS - 2509-2723 (Linking) DP - 2026 Oct 29 TI - Age-associated changes in microglia-like and satellite glial cell markers in the cochlear spiral ganglion. LID - 10.1007/s11357-026-02617-w [doi] AB - Age-related hearing loss (ARHL) and noise-induced hearing loss (NIHL) are the most prevalent forms of acquired auditory impairment. However, whether the cochlear immune microenvironment differs between these conditions, and whether it can be therapeutically modulated, remains largely unknown. Satellite glial cells (SGCs), which ensheath spiral ganglion (SG) neurons, have not traditionally been considered immune-competent, and their role in cochlear immune remodeling has not been examined. Iba1(+) microglia-like cells and Sox2(+) SGCs were systematically characterized across four groups: young control (CTRL), young NIHL, aged vehicle-treated (Veh), and aged PLX5622-treated (PLX) mice, using double immunofluorescence staining for Iba1, Tmem119, Sox2, and CD74. In young mice, NIHL did not significantly alter total Iba1(+) cell density or Iba1(+)/CD74(+) co-expression in the SG compared to CTRL. In contrast, aged Veh mice showed a significant increase in total Iba1(+) cell density and in Iba1(+)/Tmem119(+), Iba1(+)/CD74(+) and Sox2(+)/CD74(+) cell densities relative to CTRL, with a similar upward trend for Sox2(+)/Tmem119(+) cells that did not reach statistical significance after multiple-comparison correction. A 7-day course of the PLX5622 significantly reduced total Iba1(+), Iba1(+)/Tmem119(+), Iba1(+)/CD74(+), and Sox2(+)/CD74(+) cell densities in aged PLX mice. These findings identify age-associated differences in immune- and glia-associated marker-positive cell densities within the cochlear SG and show that selected marker-positive populations were reduced following PLX5622 treatment. Although age and genotype were confounded and PLX5622 may exert both local and systemic effects, these observations highlight the cochlear SG immune microenvironment as a potential area for further investigation in ARHL. CI - (c) 2026. The Author(s). FAU - Park, Bohyeon AU - Park B AD - Department of Anatomy and Cell Biology, Seoul National University College of Medicine, 103, Daehak-Ro, Jongno-Gu, Seoul, 03080, Republic of Korea. FAU - Jeon, Jong Chan AU - Jeon JC AD - Department of Anatomy and Cell Biology, Seoul National University College of Medicine, 103, Daehak-Ro, Jongno-Gu, Seoul, 03080, Republic of Korea. FAU - Kim, Do Eun AU - Kim DE AD - Department of Anatomy and Cell Biology, Seoul National University College of Medicine, 103, Daehak-Ro, Jongno-Gu, Seoul, 03080, Republic of Korea. FAU - Park, NaHae AU - Park N AD - Department of Anatomy and Cell Biology, Seoul National University College of Medicine, 103, Daehak-Ro, Jongno-Gu, Seoul, 03080, Republic of Korea. FAU - Kim, So Young AU - Kim SY AUID- ORCID: 0000-0002-7361-4930 AD - Department of Anatomy and Cell Biology, Seoul National University College of Medicine, 103, Daehak-Ro, Jongno-Gu, Seoul, 03080, Republic of Korea. sossi81@snu.ac.kr. AD - Sensory Organ Research Institute, Seoul National University Medical Research Center, Seoul, Republic of Korea. sossi81@snu.ac.kr. AD - Neuroscience Research Institute, Seoul National University Medical Research Center, Seoul, Republic of Korea. sossi81@snu.ac.kr. LA - eng GR - 800-20250270/College of Medicine, Seoul National University/ GR - 800-20250440/College of Medicine, Seoul National University/ GR - RS-2025-25459502/National Research Foundation (KR)/ PT - Journal Article DEP - 20261029 PL - Switzerland TA - Geroscience JT - GeroScience JID - 101686284 SB - IM OTO - NOTNLM OT - Cochlear inflammation OT - Cochlear spiral ganglion OT - Microglia-like cells OT - Satellite glial cells OT - Tmem119 COIS- Declarations. Role of the funder/sponsor: The KHIDI and NRF had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication. Conflict of interest: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:33 PHST- 2026/04/29 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:33 [entrez] AID - 10.1007/s11357-026-02617-w [pii] AID - 10.1007/s11357-026-02617-w [doi] PST - aheadofprint SO - Geroscience. 2026 Oct 29. doi: 10.1007/s11357-026-02617-w. PMID- 42955648 OWN - NLM STAT- Publisher LR - 20261029 IS - 1348-2246 (Electronic) IS - 0910-6340 (Linking) DP - 2026 Oct 28 TI - Two-color, laser-induced fluorometry for evaluating the efficiency of click chemistry-based labeling of extracellular vesicles. LID - 10.1007/s44211-026-00979-x [doi] AB - Extracellular vesicles (EVs), including exosomes, have attracted considerable attention as promising biomarkers for the early diagnosis and prognosis of cancer. In this study we evaluated the efficiency of a click chemistry-based EV labeling method using a two-color laser-induced fluorometry (LIF) system. During cell culture, cellular membranes were metabolically functionalized with azide groups, and the released EVs were subsequently labeled with Cy5 via click chemistry. The collected EVs were then stained with a phycoerythrin-conjugated anti-CD63 antibody (PE-anti-CD63) to achieve dual labeling. A two-color LIF system equipped with 488- and 635-nm lasers was used to distinguish Cy5-labeled, CD63-positive EVs from Cy5-unlabeled, CD63-positive EVs. Because PE and Cy5 are selectively excited at 488 and 635 nm, respectively, Cy5-labeled, CD63-positive EVs generated coincident dual-color fluorescence, whereas Cy5-unlabeled, CD63-positive EVs emitted fluorescence only upon 488-nm excitation. Using this platform, we investigated the effect that serum concentration in the culture medium exerts on click-chemistry labeling efficiency for CD63-positive EVs. The highest labeling efficiency for both HeLa and A549 cells was obtained in medium supplemented with 10% fetal bovine serum. These findings demonstrate the utility of the proposed two-color LIF system for quantitatively evaluating the click-chemistry labeling efficiency of CD63-positive EVs under different cell-culture conditions. CI - (c) 2026. The Author(s). FAU - Takaya, Arisa AU - Takaya A AD - Department of Chemistry, Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama, 700-8530, Japan. FAU - Kaneta, Takashi AU - Kaneta T AUID- ORCID: 0000-0001-9076-3906 AD - Department of Chemistry, Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama, 700-8530, Japan. kaneta@okayama-u.ac.jp. LA - eng PT - Journal Article DEP - 20261028 PL - Switzerland TA - Anal Sci JT - Analytical sciences : the international journal of the Japan Society for Analytical Chemistry JID - 8511078 SB - IM OTO - NOTNLM OT - Click chemistry OT - Exosome OT - Extracellular vesicle OT - Fluorescent labeling OT - Laser-induced fluorescence COIS- Declarations. Conflict of interest: The authors declare that they have no known competing financial interests or personal relationships that could appear to influence the work reported in this paper. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:33 PHST- 2026/07/21 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:33 [entrez] AID - 10.1007/s44211-026-00979-x [pii] AID - 10.1007/s44211-026-00979-x [doi] PST - aheadofprint SO - Anal Sci. 2026 Oct 28. doi: 10.1007/s44211-026-00979-x. PMID- 42955643 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 3101 DP - 2027 TI - Engineered Antibody-Like Bivalent Binders for Manipulating Receptor Functions. PG - 281-290 LID - 10.1007/978-1-0716-5687-7_20 [doi] AB - Intracellular signal transduction mediated by most cell-surface single-pass transmembrane receptors depends on the formation of receptor dimers with defined geometries. Consequently, bivalent binders capable of positioning two receptor molecules in specific spatial configurations can serve as useful molecular tools for manipulating receptor activity. We previously developed Mirabodies, antibody-like bivalent binders generated by grafting peptide-derived receptor-binding sequences into loop regions of the human IgG1 Fc scaffold. This format combines the high efficiency of macrocyclic peptide selection in identifying high-affinity target-binding peptide sequences with the high producibility, structural rigidity, and favorable pharmacokinetic properties of Fc-based molecules. In this chapter, we describe practical protocols for the design, expression, and purification of Mirabodies using mammalian cell expression systems. These methods provide a straightforward workflow for converting receptor-binding peptides into functional Fc-based agonists or antagonists. CI - (c) 2027. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Arimori, Takao AU - Arimori T AD - Institute for Protein Research, The University of Osaka, Suita, Osaka, Japan. arimori@protein.osaka-u.ac.jp. FAU - Takagi, Junichi AU - Takagi J AD - Institute for Protein Research, The University of Osaka, Suita, Osaka, Japan. LA - eng PT - Journal Article PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 RN - 0 (Immunoglobulin G) RN - 0 (Immunoglobulin Fc Fragments) RN - 0 (Peptides) SB - IM MH - Humans MH - *Protein Engineering/methods MH - Animals MH - Immunoglobulin G/chemistry/genetics/metabolism MH - Protein Binding MH - Immunoglobulin Fc Fragments/genetics/chemistry/metabolism MH - HEK293 Cells MH - Peptides/chemistry/metabolism OTO - NOTNLM OT - Antibody OT - Bivalent binder OT - Fc OT - Lasso-grafting OT - Macrocyclic peptide OT - Mirabody OT - Receptor simerization COIS- Conflict of Interest. The authors have no conflicts of interest to declare that are relevant to the content of this chapter. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:33 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:33 [entrez] AID - 10.1007/978-1-0716-5687-7_20 [doi] PST - ppublish SO - Methods Mol Biol. 2027;3101:281-290. doi: 10.1007/978-1-0716-5687-7_20. PMID- 42955636 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1940-6029 (Electronic) IS - 1064-3745 (Linking) VI - 3101 DP - 2027 TI - Wash-Free Live-Cell Imaging Using Homogeneous Immunosensors. PG - 201-209 LID - 10.1007/978-1-0716-5687-7_13 [doi] AB - Imaging membrane proteins on the cell surface is crucial for studying cellular functions involving their mobility, localization, and clustering. Conventional antibody-based fluorescence imaging requires multiple washing steps to reduce background signals from unbound fluorescent antibodies. These steps stress living cells and potentially alter their characteristics and behavior. To address this, we propose a protocol for wash-free fluorescence imaging using an immunosensor, the Quenchbody (Q-body), which emits fluorescence upon antigen binding, eliminating the need for washing steps. In this chapter, we introduce the preparation of a Q-body against a cancer biomarker, human epidermal growth factor receptor 2 (HER2), and its application in live-cell imaging by simply adding to the culture medium without washing. This tool offers a minimally invasive and efficient approach to real-time membrane protein analysis. Importantly, because the antibody component is exchangeable with other target-specific antibodies, Q-bodies are adaptable for detecting a wide range of membrane proteins in live-cell imaging. CI - (c) 2027. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Yasuda, Takanobu AU - Yasuda T AD - Laboratory for Chemistry and Life Science, Institute of Integrated Research, Institute of Science Tokyo, Yokohama, Kanagawa, Japan. FAU - Ueno, Yoshiyuki AU - Ueno Y AD - Laboratory for Chemistry and Life Science, Institute of Integrated Research, Institute of Science Tokyo, Yokohama, Kanagawa, Japan. FAU - Kitaguchi, Tetsuya AU - Kitaguchi T AD - Laboratory for Chemistry and Life Science, Institute of Integrated Research, Institute of Science Tokyo, Yokohama, Kanagawa, Japan. kitaguchi.t.1889@m.isct.ac.jp. LA - eng PT - Journal Article PL - United States TA - Methods Mol Biol JT - Methods in molecular biology (Clifton, N.J.) JID - 9214969 RN - EC 2.7.10.1 (Erb-b2 Receptor Tyrosine Kinases) RN - EC 2.7.10.1 (ERBB2 protein, human) SB - IM MH - Humans MH - *Biosensing Techniques/methods MH - Erb-b2 Receptor Tyrosine Kinases/metabolism/immunology MH - Microscopy, Confocal/methods MH - Immunoassay/methods MH - *Optical Imaging/methods MH - *Molecular Imaging/methods OTO - NOTNLM OT - Antibody OT - Confocal microscopy OT - Fluorescence quenching OT - Homogeneous immunosensor OT - Wash-free COIS- Competing Interests. T.Y. and T.K. have received honoraria from HikariQ Health Inc. for another unrelated project. Y.U. is an employee of HikariQ Health Inc. and is enrolled as a doctoral student at the Institute of Science Tokyo. HikariQ Health Inc. was not involved in the study design, data collection, analysis, interpretation, or decision to publish. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:33 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:33 [entrez] AID - 10.1007/978-1-0716-5687-7_13 [doi] PST - ppublish SO - Methods Mol Biol. 2027;3101:201-209. doi: 10.1007/978-1-0716-5687-7_13. PMID- 42955615 OWN - NLM STAT- Publisher LR - 20261029 IS - 1573-3327 (Electronic) IS - 0009-398X (Linking) DP - 2026 Oct 29 TI - Sibling Outcomes Among Probands with Autism Spectrum Disorder: A Population-wide Longitudinal Cohort Study. LID - 10.1007/s10578-026-02112-5 [doi] AB - Living with an Autistic sibling presents unique challenges for their siblings. Previous literature reports increased psychiatric problems in siblings of autistic probands, but other areas of functioning remain underexplored at the population-level. This study investigates whether these siblings have different rates of mental disorders, hospitalized suicide attempts, psychotropic medication prescriptions, physical health burden, and educational outcomes against siblings of Down Syndrome probands and siblings of individuals without neurodevelopmental disorders (Neurotypical). Using population-based administrative data, three longitudinal matched cohorts evaluated outcomes over a 10-24-year follow-up. After accounting for covariates, siblings of autistic probands (N = 5,409) evidenced increased risk of all mental disorders except substance use disorder versus siblings of Neurotypical probands (N = 328,015), and increased risk of select disorders versus siblings of Down Syndrome probands (N = 3,952). Siblings of autistic probands were more likely to be prescribed psychotropic medications than either comparator and more likely to experience developmental delays than siblings of Neurotypical probands. CI - (c) 2026. The Author(s). FAU - Katz, Cara AU - Katz C AD - Department of Psychiatry, University of Manitoba, Winnipeg, MB, Canada. FAU - Spiwak, Rae AU - Spiwak R AD - Department of Psychiatry, University of Manitoba, Winnipeg, MB, Canada. AD - Department of Surgery, University of Manitoba, Winnipeg, MB, Canada. FAU - Tan, Qier AU - Tan Q AD - Manitoba Centre for Health Policy, University of Manitoba, Winnipeg, MB, Canada. FAU - Roos, Leslie AU - Roos L AD - Department of Psychology, University of Manitoba, Winnipeg, MB, Canada. FAU - McHardy, Robert J W AU - McHardy RJW AD - Department of Psychology, University of Manitoba, Winnipeg, MB, Canada. FAU - Katz, Laurence Y AU - Katz LY AD - Department of Psychiatry, University of Manitoba, Winnipeg, MB, Canada. lkatz@hsc.mb.ca. AD - Manitoba Centre for Health Policy, University of Manitoba, Winnipeg, MB, Canada. lkatz@hsc.mb.ca. AD - , PZ-162, 771 Bannatyne Ave, Winnipeg, MB, R3E 3N4, Canada. lkatz@hsc.mb.ca. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Child Psychiatry Hum Dev JT - Child psychiatry and human development JID - 1275332 SB - IM OTO - NOTNLM OT - Administrative data OT - Autism Spectrum Disorder OT - Longitudinal cohort OT - Mental disorders OT - Sibling outcomes COIS- Declarations. Competing interests: The authors declare no competing interests. Ethics Approval: Ethical approvals were obtained from the University of Manitoba Health Research Ethics Board (HREB), Health Information and Privacy Committee (HIPC) of Manitoba Health, and necessary agencies whose data was accessed. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:33 PHST- 2026/03/29 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:33 [entrez] AID - 10.1007/s10578-026-02112-5 [pii] AID - 10.1007/s10578-026-02112-5 [doi] PST - aheadofprint SO - Child Psychiatry Hum Dev. 2026 Oct 29. doi: 10.1007/s10578-026-02112-5. PMID- 42955606 OWN - NLM STAT- Publisher LR - 20261029 IS - 1865-7265 (Electronic) IS - 1865-7265 (Linking) DP - 2026 Oct 28 TI - Gastric epithelial neoplasm of fundic gland mucosa lineage with autoimmune gastritis: a case report with molecular analysis. LID - 10.1007/s12328-026-02463-5 [doi] AB - Gastric epithelial neoplasm of fundic gland mucosa lineage (GEN-FGML) is a rare gastric neoplasm that typically arises in non-atrophic fundic gland mucosa and is generally unrelated to Helicobacter pylori infection. In contrast, autoimmune gastritis (AIG) is characterized by corpus-predominant atrophy and destruction of fundic glands. GEN-FGML associated with AIG is rare, and its molecular characteristics remain unclear. A 60-year-old woman underwent esophagogastroduodenoscopy during a routine health checkup. Endoscopic examination revealed severe corpus-predominant atrophy consistent with AIG and a 5-mm whitish elevated lesion in the upper gastric body. Laboratory findings showed hypergastrinemia and positivity for anti-parietal cell antibodies. The lesion was successfully resected by endoscopic submucosal dissection. Histopathological examination demonstrated an oxyntic gland adenoma composed predominantly of chief cell-like cell. Immunohistochemically, the tumor cells were positive for pepsinogen I and MUC6 and showed nuclear beta-catenin expression. Residual fundic glands were identified adjacent to the tumor despite advanced background atrophy. Mutational analyses revealed no pathogenic mutations in GNAS, KRAS, or BRAF. This case suggests that GEN-FGML can arise from residual fundic glands preserved in AIG and may share molecular features with conventional GEN-FGML. CI - (c) 2026. The Author(s), under exclusive licence to Japanese Society of Gastroenterology. FAU - Nagahashi, Takayuki AU - Nagahashi T AUID- ORCID: 0000-0001-9098-8574 AD - Department of Gastroenterology, Southern-Tohoku General Hospital, 7-115 Yatsuyamada, Koriyama-shi, Fukushima, Japan. takayuki.nagahashi@mt.strins.or.jp. AD - Department of Minimally Invasive Surgical and Medical Oncology, Fukushima Medical University, Fukushima, Japan. takayuki.nagahashi@mt.strins.or.jp. FAU - Hamada, Koichi AU - Hamada K AD - Department of Gastroenterology, Southern-Tohoku General Hospital, 7-115 Yatsuyamada, Koriyama-shi, Fukushima, Japan. AD - Department of Minimally Invasive Surgical and Medical Oncology, Fukushima Medical University, Fukushima, Japan. FAU - Sugai, Tamotsu AU - Sugai T AD - Department of Pathology, Southern-Tohoku General Hospital, Koriyama, Japan. FAU - Horikawa, Yoshinori AU - Horikawa Y AD - Department of Gastroenterology, Southern-Tohoku General Hospital, 7-115 Yatsuyamada, Koriyama-shi, Fukushima, Japan. FAU - Techigawara, Kae AU - Techigawara K AD - Department of Gastroenterology, Southern-Tohoku General Hospital, 7-115 Yatsuyamada, Koriyama-shi, Fukushima, Japan. AD - Department of Minimally Invasive Surgical and Medical Oncology, Fukushima Medical University, Fukushima, Japan. FAU - Ishikawa, Masafumi AU - Ishikawa M AD - Department of Gastroenterology, Southern-Tohoku General Hospital, 7-115 Yatsuyamada, Koriyama-shi, Fukushima, Japan. FAU - Watanabe, Haruki AU - Watanabe H AD - Department of Gastroenterology, Southern-Tohoku General Hospital, 7-115 Yatsuyamada, Koriyama-shi, Fukushima, Japan. FAU - Uesugi, Noriyuki AU - Uesugi N AD - Department of Pathology, Southern-Tohoku General Hospital, Koriyama, Japan. FAU - Honda, Michitaka AU - Honda M AD - Department of Minimally Invasive Surgical and Medical Oncology, Fukushima Medical University, Fukushima, Japan. AD - Department of Surgery, Southern-Tohoku General Hospital, Koriyama, Japan. LA - eng PT - Journal Article DEP - 20261028 PL - Japan TA - Clin J Gastroenterol JT - Clinical journal of gastroenterology JID - 101477246 SB - IM OTO - NOTNLM OT - Autoimmune gastritis OT - Gastric epithelial neoplasm of fundic gland mucosa lineage OT - Molecular analyses COIS- Declarations. Conflict of Interest: The authors declare that they have no conflict of interest. Human/Animal Rights: All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2013. Informed Consent: Written informed consent was obtained from the patient for publication of this case report and accompanying images. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:32 PHST- 2026/07/15 00:00 [received] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:32 [entrez] AID - 10.1007/s12328-026-02463-5 [pii] AID - 10.1007/s12328-026-02463-5 [doi] PST - aheadofprint SO - Clin J Gastroenterol. 2026 Oct 28. doi: 10.1007/s12328-026-02463-5. PMID- 42955601 OWN - NLM STAT- Publisher LR - 20261029 IS - 1573-2584 (Electronic) IS - 0301-1623 (Linking) DP - 2026 Oct 28 TI - Varicocele embolization using n-butyl cyanoacrylate glue: a systematic review and meta-analysis. LID - 10.1007/s11255-026-05426-4 [doi] AB - PURPOSE: Varicocele, a dilation of the pampiniform plexus, is a common and potentially reversible cause of male infertility, contributing to up to 50% of cases. Symptoms may include scrotal pain, heaviness, and cosmetic concerns. Percutaneous embolization is a minimally invasive treatment with high success rates. N-Butyl cyanoacrylate (NBCA)/N-butyl cyanoacrylate-methacryloxysulfolane (NBCA-MS) glue is increasingly utilized due to improved safety. This meta-analysis evaluates the technical and clinical success of NBCA/NBCA-MS embolization in varicocele-related disease. METHODS: A systematic review and proportional meta-analysis was conducted per PRISMA guidelines (PROSPERO: CRD420261332073). PubMed, Embase, and CENTRAL were searched up to March 4, 2026. Retrospective and prospective studies reporting NBCA/NBCA-MS embolization outcomes were included. Primary outcomes were technical and clinical success; secondary outcomes included recurrence. Proportions were pooled using a random-effects model with Freeman-Tukey transformation, and heterogeneity was assessed using I(2). RESULTS: Eleven studies (five prospective, six retrospective) composed of 548 patients (10 cohorts) were included for analysis. Technical success was 100% across nine studies, with recurrence (eight studies) reported at 1% (95% CI 0-5%; I(2)=72.35%), fertility improvement (three studies) at 68% (95% CI 0.51-0.83; I(2)=70.3%), and pain improvement (three studies) was found to be 97% (95% CI 0.75-1.00; I(2)=79.8%) across reported cohorts.. CONCLUSION: Overall, embolization studies reported high technical success and low recurrence rates, as well as high reported rates of fertility and pain improvement; however, these findings should be interpreted cautiously given the small number of contributing studies and substantial between-study heterogeneity. CI - (c) 2026. The Author(s). FAU - Mac Curtain, Benjamin M AU - Mac Curtain BM AD - Northwell Health at Lenox Hill Hospital, Department of Urology, Zucker School of Medicine at Hofstra/Northwell, New York, United States. FAU - Cronin, Padraig AU - Cronin P AD - School of Medicine, University College Cork, Cork, Ireland. FAU - Temperley, Hugo C AU - Temperley HC AD - Department of Radiology, St. James's Hospital, Dublin, Ireland. AD - Trinity St James Cancer Institute, St James Hospital, Dublin 8, Ireland. FAU - Deshwal, Avinash AU - Deshwal A AD - Royal College of Surgeons in Ireland, Dublin 2, Ireland. AD - Department of Surgery, Beaumont Hospital, Dublin 9, Ireland. FAU - Nicaise, Edouard AU - Nicaise E AD - Northwell Health at Lenox Hill Hospital, Department of Urology, Zucker School of Medicine at Hofstra/Northwell, New York, United States. FAU - Klyde, Daniel AU - Klyde D AD - Northwell Health at Lenox Hill Hospital, Department of Urology, Zucker School of Medicine at Hofstra/Northwell, New York, United States. FAU - Singh, Adityabikram AU - Singh A AD - Northwell Health at Lenox Hill Hospital, Department of Urology, Zucker School of Medicine at Hofstra/Northwell, New York, United States. FAU - Bruinsma, Joshua AU - Bruinsma J AD - Department of Urology, Sir Charles Gairdner Hospital, Perth, Australia. FAU - Calpin, Gavin G AU - Calpin GG AD - Royal College of Surgeons in Ireland, Dublin 2, Ireland. gavincalpin22@rcsi.com. FAU - Oparanozie, Arnold AU - Oparanozie A AD - Northwell Health at Lenox Hill Hospital, Department of Urology, Zucker School of Medicine at Hofstra/Northwell, New York, United States. FAU - Rastinehad, Ardeshir R AU - Rastinehad AR AD - Northwell Health at Lenox Hill Hospital, Department of Urology, Zucker School of Medicine at Hofstra/Northwell, New York, United States. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - Netherlands TA - Int Urol Nephrol JT - International urology and nephrology JID - 0262521 SB - IM OTO - NOTNLM OT - Embolization OT - Male infertility OT - N-Butyl cyanoacrylate OT - N-Butyl cyanoacrylate-methacryloxysulfolane OT - Varicocele COIS- Declarations. Conflict of interest: The authors report no conflict of interest. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:32 PHST- 2026/07/04 00:00 [received] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:32 [entrez] AID - 10.1007/s11255-026-05426-4 [pii] AID - 10.1007/s11255-026-05426-4 [doi] PST - aheadofprint SO - Int Urol Nephrol. 2026 Oct 28. doi: 10.1007/s11255-026-05426-4. PMID- 42955595 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1943-393X (Electronic) IS - 1943-3921 (Linking) VI - 88 IP - 8 DP - 2026 Oct 28 TI - The calm before the storm: Silence compresses subsequent visual time without reducing temporal precision. LID - 216 [pii] LID - 10.3758/s13414-026-03365-x [doi] AB - Silence is often conceptualized as the mere absence of auditory input; however, accumulating evidence suggests that the omission and offset of sound are processed as psychologically salient perceptual events. This study investigated whether an abrupt transition from sound to silence biases the perceived duration of subsequently presented visual stimuli. Across four psychophysical experiments, participants performed forced-choice temporal discrimination tasks in which a brief silent interval (sound offset) was inserted between a standard and a comparison stimulus. Visual stimuli presented immediately following the offset were consistently perceived as shorter in duration than physically identical stimuli presented under continuous background noise. This duration compression effect was robust across simple geometric stimuli and generalized to dynamic video content. Critically, sound offset significantly shifted the point of subjective equality (PSE) while leaving temporal sensitivity measures, including the difference limen (DL) and Weber fraction, unaffected. We also conducted additional experiments demonstrating that the effect could not be attributed to graded changes in sound intensity and persisted across various interstimulus intervals (ISIs). These findings indicate that sound offset selectively compresses perceived visual duration without degrading temporal discrimination precision, supporting the view that silence functions as a categorical contextual event that recalibrates the temporal reference framework for subsequent duration judgments. Collectively, the results highlight the role of auditory context transitions in shaping multisensory time perception. OPEN PRACTICES STATEMENT: The trial-level data for all experiments are publicly available on the Open Science Framework (OSF) at https://osf.io/3ksa8/overview . The experimental scripts and analysis code are available upon request. None of the experiments were preregistered. CI - (c) 2026. The Psychonomic Society, Inc. FAU - Furusawa, Go AU - Furusawa G AD - Graduate School of Human Relations, Keio University, 2-15-45 Mita, Minato-Ku, Tokyo, 108-8345, Japan. FAU - Kawabata, Hideaki AU - Kawabata H AUID- ORCID: 0000-0001-8132-4995 AD - Graduate School of Human Relations, Keio University, 2-15-45 Mita, Minato-Ku, Tokyo, 108-8345, Japan. kawabata@keio.jp. AD - Department of Psychology, Keio University, 2-15-45 Mita, Minato-Ku, Tokyo, 108-8345, Japan. kawabata@keio.jp. LA - eng GR - 25H01132/Japan Society for the Promotion of Science/ GR - 24H02199/Ministry of Education, Culture, Sports, Science and Technology/ PT - Journal Article DEP - 20261028 PL - United States TA - Atten Percept Psychophys JT - Attention, perception & psychophysics JID - 101495384 SB - IM MH - Humans MH - *Time Perception/physiology MH - *Auditory Perception/physiology MH - *Discrimination, Psychological MH - Female MH - *Pattern Recognition, Visual MH - Young Adult MH - Male MH - Adult MH - *Attention MH - Judgment OTO - NOTNLM OT - Crossmodal perception OT - Duration judgments OT - Silence OT - Sound offset OT - Time perception COIS- Declarations. Ethics approval: This study adhered to the principles of the Declaration of Helsinki and was approved by the Ethics Committee of Keio University (24018). Consent to participate: All participants provided written informed consent. Consent for publication: All authors have read and approved the final manuscript and consent to its publication in Attention, Perception, & Psychophysics. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:32 PHST- 2026/03/20 00:00 [received] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:32 [entrez] AID - 10.3758/s13414-026-03365-x [pii] AID - 10.3758/s13414-026-03365-x [doi] PST - epublish SO - Atten Percept Psychophys. 2026 Oct 28;88(8):216. doi: 10.3758/s13414-026-03365-x. PMID- 42955586 OWN - NLM STAT- Publisher LR - 20261029 IS - 1614-7499 (Electronic) IS - 0944-1344 (Linking) DP - 2026 Oct 29 TI - Oil recovery and characterization of storage tank bottom oily sludge via solvent extraction. LID - 10.1007/s11356-026-38184-y [doi] AB - Oily sludge is one of the most hazardous wastes generated by the petroleum industry due to its high hydrocarbon content and associated environmental risks. In this study, solvent extraction was employed to recover hydrocarbon oil from sludge collected at the base of a crude oil tank in an Algerian refinery. Cyclohexane and xylene were selected as solvents due to their efficiency in extracting oil from aliphatic, cyclic, and aromatic fractions. Residual solids were collected to assess the environmental impact. To optimize oil recovery from the sludge, the effects of physicochemical parameters, including stirring time, solvent volume, and temperature, were studied. The extracted oil was characterized using gas chromatography-mass spectrometry (GC-MS) and Fourier-transform infrared (FTIR) spectroscopy, while the residual solids were analyzed by X-ray fluorescence (XRF), X-ray diffraction (XRD), and FTIR spectroscopy. Xylene demonstrated superior extraction performance, achieving an oil recovery rate of 96.11% from crude oil sludge. Elemental analysis of the residues revealed the presence of iron, sulfur, aluminum, silicon, and calcium, with calcium being the predominant element. XRD analysis identified calcium carbonate and silicon dioxide as the main mineral phases in the solid residues. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Amraoui, Loubna AU - Amraoui L AD - Faculte des Sciences et de la Technologie, Universite Mohamed Khider de Biskra, Biskra, Algeria. AD - Laboratoire de Recherche en Genie Civil, Hydraulique, Developpement Durable et Environnement, Universite de Biskra, Biskra, Algeria. FAU - Hasseine, Abdelmalek AU - Hasseine A AD - Faculte des Sciences et de la Technologie, Universite Mohamed Khider de Biskra, Biskra, Algeria. AD - Laboratoire de Recherche en Genie Civil, Hydraulique, Developpement Durable et Environnement, Universite de Biskra, Biskra, Algeria. FAU - Bouzid, Khadidja AU - Bouzid K AD - Centre de Recherche Scientifique et Technique sur les Regions Arides (CRSTRA), Biskra, Algeria. FAU - Achouri, Ines Esma AU - Achouri IE AUID- ORCID: 0000-0001-8017-7946 AD - Faculte de Genie, Genie chimique et genie biotechnologique, Universite de Sherbrooke, Sherbrooke, Quebec, J1K 2R1, Canada. Ines.esma.Achouri@USherbrooke.ca. LA - eng PT - Journal Article DEP - 20261029 PL - Germany TA - Environ Sci Pollut Res Int JT - Environmental science and pollution research international JID - 9441769 SB - IM OTO - NOTNLM OT - Oil recovery OT - Petroleum sludge OT - Physicochemical characteristics OT - Recovery rate OT - Solvent extraction COIS- Declarations. Ethical approval: Not required. Consent to participate: This is not applicable. Consent for publication: All authors have seen and approved the submission of the manuscript. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:32 PHST- 2026/04/03 00:00 [received] PHST- 2026/08/21 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:32 [entrez] AID - 10.1007/s11356-026-38184-y [pii] AID - 10.1007/s11356-026-38184-y [doi] PST - aheadofprint SO - Environ Sci Pollut Res Int. 2026 Oct 29. doi: 10.1007/s11356-026-38184-y. PMID- 42955581 OWN - NLM STAT- Publisher LR - 20261029 IS - 1614-7499 (Electronic) IS - 0944-1344 (Linking) DP - 2026 Oct 29 TI - Cross-linked chitosan-nicotinic acid beads (CLC-NAB)-a novel adsorbent for the removal of Hg(II) ions from aqueous medium. LID - 10.1007/s11356-026-38354-y [doi] AB - An adsorbent, cross-linked chitosan-nicotinic acid beads (CLC-NAB) was fabricated in a sustainable green method and used effectively for the selective adsorption of bivalent mercury ions, Hg(II) from aqueous solutions. The CLC-NAB were characterized by swelling study, FTIR spectroscopy, SEM and elemental analysis. Optimum conditions for the adsorption process were determined by varying various operational parameters- initial pH, adsorbent dosage, initial Hg(II) concentrations, contact time, temperature. Kinetic studies showed that the Hg(II) adsorption follows both pseudo second-order and intraparticle diffusion kinetic model. The adsorption experimental data were well fitted to Redlich-Peterson isotherm model and the monolayer adsorption capacity is 1060.2 mg/g. Complete removal of toxic Hg(II) ions was obtained using 36 g/L of CLC-NAB at pH 5. A 100% desorption of the adsorbed mercury ions was attained with 1 M NaCl and 1 M NaNO(3) as desorbing agents from mercury loaded CLC-NAB. The CLC-NAB exhibit excellent reusability upto five regeneration cycles. The environment-friendly CLC-NAB with good stability can be used as a promising adsorbent for the removal of Hg(II) from aqueous solutions. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Manna, Arpita AU - Manna A AD - Department of Chemistry, Prabhu Jagatbandhu College, Howrah, 711302, India. FAU - Banerjee, Kakoli AU - Banerjee K AUID- ORCID: 0000-0002-7862-1259 AD - Department of Chemistry, Prabhu Jagatbandhu College, Howrah, 711302, India. kakolika@gmail.com. LA - eng PT - Journal Article DEP - 20261029 PL - Germany TA - Environ Sci Pollut Res Int JT - Environmental science and pollution research international JID - 9441769 SB - IM OTO - NOTNLM OT - Adsorption OT - Chitosan OT - Cross-linking OT - Hydrogels OT - Mercury removal OT - Nicotinic acid COIS- Declarations. Ethical approval: This is not applicable. Consent to participate: This is not applicable. Consent for publication: This is not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:32 PHST- 2026/06/15 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:32 [entrez] AID - 10.1007/s11356-026-38354-y [pii] AID - 10.1007/s11356-026-38354-y [doi] PST - aheadofprint SO - Environ Sci Pollut Res Int. 2026 Oct 29. doi: 10.1007/s11356-026-38354-y. PMID- 42955577 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1943-393X (Electronic) IS - 1943-3921 (Linking) VI - 88 IP - 8 DP - 2026 Oct 28 TI - The role of inhibition in task switching: Evidence from an n-back task. LID - 214 [pii] LID - 10.3758/s13414-026-03359-9 [doi] AB - Task switching is a well-established paradigm for assessing cognitive flexibility. However, the precise role of inhibition in task switching remains unclear. This study investigates the effects of inhibition on switch costs in tasks requiring participants to judge whether the magnitude (small vs. large) or parity (odd vs. even) of a number was the same as that presented in previous trials. To this end, an n-back task was embedded in the task-switching paradigm. The results of Experiment 1 demonstrated that, in comparison to the 1-back condition, switch costs were significantly reduced in the 2-back condition, which involved an increased inhibition process. The selective increase in inhibition in some trials in the 1-back condition (Experiment 2) and the carryover of inhibition (Experiment 3) significantly reduced the switch costs. These findings indicate that the enhanced inhibition of task-irrelevant information can facilitate cognitive flexibility in task switching and reduce switch costs, providing novel empirical evidence for the inhibition mechanism underlying task switching. CI - (c) 2026. The Psychonomic Society, Inc. FAU - Cao, Bihua AU - Cao B AD - Institute of Brain and Psychological Sciences, Sichuan Normal University, Jing'an Avenue, Chengdu, 610066, China. FAU - Liu, Gege AU - Liu G AD - School of Psychology, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, 330022, China. FAU - Fu, Meiling AU - Fu M AD - School of Psychology, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, 330022, China. FAU - Pan, Chenyu AU - Pan C AD - School of Psychology, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, 330022, China. FAU - Li, Fuhong AU - Li F AD - School of Psychology, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, 330022, China. lifh@foxmail.com. LA - eng GR - 32360201/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261028 PL - United States TA - Atten Percept Psychophys JT - Attention, perception & psychophysics JID - 101495384 SB - IM MH - Humans MH - *Inhibition, Psychological MH - *Memory, Short-Term/physiology MH - *Cognitive Flexibility MH - *Attention/physiology MH - Reaction Time MH - *Pattern Recognition, Visual MH - Female MH - Young Adult MH - Male MH - Judgment MH - Adult MH - Size Perception OTO - NOTNLM OT - Inhibition OT - Switch costs OT - Task switching OT - Working memory COIS- Declarations. Ethics approval: This study was performed in line with the principles of the Declaration of Helsinki and the American Psychological Association's ethical standards. This study was approved by the Research Ethics Committee of the School of Psychology, Jiangxi Normal University. Consent to participate: Informed consent was obtained from all participants included in this study. Consent for publication: The authors consent to publication of this work. Conflict of interest: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Preregistration: This study was not preregistered. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:31 PHST- 2026/04/17 00:00 [received] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:31 [entrez] AID - 10.3758/s13414-026-03359-9 [pii] AID - 10.3758/s13414-026-03359-9 [doi] PST - epublish SO - Atten Percept Psychophys. 2026 Oct 28;88(8):214. doi: 10.3758/s13414-026-03359-9. PMID- 42955573 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1941-6636 (Electronic) VI - 57 IP - 1 DP - 2026 Oct 28 TI - Yttrium-90 Radioembolization for Hepatocellular Carcinoma: Clinical Evidence, Evolving Indications, and Research-Frontier Mapping. LID - 246 [pii] LID - 10.1007/s12029-026-01596-7 [doi] AB - Yttrium-90 (Y-90) radioembolization, also termed selective internal radiation therapy (SIRT) or transarterial radioembolization (TARE), is used across the hepatocellular carcinoma (HCC) treatment spectrum as locoregional therapy, bridging or downstaging treatment, radiation segmentectomy, and an emerging partner for systemic therapy. SARAH and SIRveNIB did not show overall survival superiority over sorafenib; DOSISPHERE-01 supported personalized dosimetry; and LEGACY/RASER strengthened radiation segmentectomy in selected solitary HCC. Recent 2025-2026 studies have updated comparative TARE/TACE evidence and combination strategies. We synthesize clinical evidence by indication and integrate a bibliometric analysis of 1,472 high-specificity WoSCC-SCIE records through June 2026 as a triangulation tool, comparing research-frontier signals with unresolved clinical evidence gaps. These signals do not establish efficacy, but they help prioritize trials of Y-90 plus immunotherapy, standardized personalized dosimetry, and contemporary comparisons against TACE or current systemic therapy. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Luo, Junpeng AU - Luo J AUID- ORCID: 0000-0002-9253-9841 AD - Translational Medical Center, Huaihe Hospital, Henan University, Kaifeng, Henan, China. hiccp@henu.edu.cn. FAU - Qi, Xiao AU - Qi X AD - Department of Radiology, Huaihe Hospital, Henan University, Kaifeng, Henan, China. FAU - Wang, Yandan AU - Wang Y AD - Department of Otolaryngology, Huaihe Hospital, Henan University, Kaifeng, Henan, China. FAU - Huang, Zhimei AU - Huang Z AD - Department of Minimally Invasive Interventional Radiology, Sun Yat-sen University Cancer Center, Dongfeng East Road 651, Guangzhou, Guangdong Province, 510260, China. FAU - Chen, Tiandong AU - Chen T AD - Department of Pathology, Henan Cancer Hospital, Zhengzhou, Henan, China. LA - eng GR - 25A320014/Key Scientific Research Project of Higher Education Institutions/ GR - SBGJ202302093/Henan Provincial Medical Science and Technology Public Relations Program, Provincial Ministerial Co-Construction Key Project/ GR - LHGJ20240406/Henan Provincial Medical Science and Technology Research Project/ PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - J Gastrointest Cancer JT - Journal of gastrointestinal cancer JID - 101479627 RN - 0 (Yttrium Radioisotopes) RN - 1K8M7UR6O1 (Yttrium-90) SB - IM MH - Humans MH - *Liver Neoplasms/therapy/mortality/pathology MH - *Yttrium Radioisotopes/therapeutic use/administration & dosage MH - *Carcinoma, Hepatocellular/therapy/mortality/pathology/radiotherapy MH - *Radioembolization, Therapeutic/methods OTO - NOTNLM OT - Hepatocellular carcinoma OT - Personalized dosimetry OT - Radiation segmentectomy OT - Selective internal radiation therapy OT - Yttrium-90 radioembolization COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:31 PHST- 2026/07/06 00:00 [received] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:31 [entrez] AID - 10.1007/s12029-026-01596-7 [pii] AID - 10.1007/s12029-026-01596-7 [doi] PST - epublish SO - J Gastrointest Cancer. 2026 Oct 28;57(1):246. doi: 10.1007/s12029-026-01596-7. PMID- 42955562 OWN - NLM STAT- Publisher LR - 20261029 IS - 2190-3883 (Electronic) IS - 1234-1983 (Linking) DP - 2026 Oct 29 TI - Combining ability and heat-stress response in rice hybrids derived from mutation-derived M(6) lines. LID - 10.1007/s13353-026-01126-y [doi] AB - Elevated temperatures are the main challenge to rice agriculture in Pakistan and around the world because rice production is highly susceptible to heat stress, demanding the development of genotypes that are phenotypically heat stress-responsive. High temperatures throughout the rice-growing season are becoming an increasing concern in Pakistan, especially in heat-prone areas where exposure during the reproductive stage can lower spikelet fertility, grain filling, and final yield. As a result, advanced mutant generations may provide valuable breeding material for determining phenotypic variation linked to yield stability and heat-stress response. This was achieved through the implementation of a lines x tester mating design using mutation-derived, advanced and morphologically uniform M(6) parental genotypes resulting in 32 F(1) hybrids from 12 parental genotypes, which were then evaluated in both normal and heat-stressed conditions. Through gamma-ray-induced mutagenesis at 150 Gy, the parental genotypes were developed from a Basmati-type fragrant rice background and progressed to the M(6) generation. However, limited information is reported regarding the application of mutation-derived M(6) rice accessions in line x tester analysis under field-induced reproductive-stage heat stress, especially when combining ability, heterosis, stress indices, and multivariate analysis are assessed together. Plant height, panicle length, number of tillers per plant, thousand grain weight, days to flowering, spikelet fertility, flag leaf area, number of grains per panicle, and harvest index were recorded. Furthermore, genotypes were assessed using principal component analysis (PCA), the Stress Tolerance Index (STI), Heat Tolerance Index (HTI), and the Stress Susceptibility Index (SSI). There is significant variation (p < 0.01) in yield-related traits between genotypes and their partition, including general combining ability (GCA) and specific combining ability (SCA), parents, crosses, and their interaction. In contrast to normal conditions, heat stress significantly reduced grain production and all traits in parents and hybrids. The phenotypic heat-stress response was further supported by targeted qRT-PCR analysis of selected HSP70 and HSFA2 gene family members in selected M(6) parental genotypes, which revealed differential regulation under heat-stress conditions. Additionally, for grain yield and all traits, the majority of parents and hybrids strongly prefer GCA and SCA effects respectively. Accessions 6511, 6543, and 7201 exhibit the best mean performances, stress tolerance index (STI), and desired GCA effects. These parents can be utilized for developing superior rice hybrids under heat stress, based on principal component analysis (PCA). For grain yield and majority traits under heat stress, the hybrid combinations 6511 x 7201, 6543 x 7218, 6561 x 7218, and 6696 x 7218 were found to be good specific combiners. Future breeding programs could make use of the identified genotypes and their traits to increase rice grain yield and heat-stress response. CI - (c) 2026. The Author(s), under exclusive licence to Institute of Plant Genetics Polish Academy of Sciences. FAU - Iqbal, Shabana AU - Iqbal S AD - Department of Plant Breeding & Genetics, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan. Shabanaiqbal930@gmail.com. FAU - Naeem, Muhammad AU - Naeem M AD - Department of Plant Breeding & Genetics, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan. FAU - Raza, Hassan AU - Raza H AD - Department of Plant Breeding & Genetics, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan. FAU - Noureldeen, Ahmed AU - Noureldeen A AD - Department of Biology, College of Sciences, Taif University, Taif, 21944, Saudi Arabia. FAU - T Al Kashgry, Najla Amin AU - T Al Kashgry NA AD - Department of Biology, College of Sciences, Taif University, Taif, 21944, Saudi Arabia. FAU - Aloufi, Salman AU - Aloufi S AD - Department of Biotechnology, College of Sciences, Taif University, Taif, 21944, Saudi Arabia. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - J Appl Genet JT - Journal of applied genetics JID - 9514582 SB - IM OTO - NOTNLM OT - Cluster analysis OT - Combining ability OT - Genetic parameters OT - HSFA2 OT - HSP70 OT - Heat Tolerance Index (HTI) OT - Heat map OT - Heat stress OT - Line x tester OT - Principal Component Analysis OT - Rice OT - Stress Susceptibility Index (SSI) OT - Stress Tolerance Index (STI) OT - qRT-PCR COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:30 PHST- 2026/04/28 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:30 [entrez] AID - 10.1007/s13353-026-01126-y [pii] AID - 10.1007/s13353-026-01126-y [doi] PST - aheadofprint SO - J Appl Genet. 2026 Oct 29. doi: 10.1007/s13353-026-01126-y. PMID- 42955557 OWN - NLM STAT- Publisher LR - 20261029 IS - 1936-0541 (Electronic) IS - 1936-0533 (Linking) DP - 2026 Oct 29 TI - Comparative analysis of ctDNA-MRD and MVI in predicting postoperative recurrence of hepatocellular carcinoma: a prospective multicenter cohort study. LID - 10.1007/s12072-026-11163-8 [doi] AB - BACKGROUND: Microvascular invasion (MVI) predicts recurrence after curative hepatocellular carcinoma (HCC) resection, but its accuracy is limited. Dynamic circulating tumor DNA (ctDNA)-minimal residual disease (MRD) monitoring has strong predictive value in solid tumors, yet head-to-head comparisons with MVI in HCC are lacking. METHODS: This prospective, multicenter cohort study enrolled 152 patients with HCC in Barcelona Clinic Liver Cancer stages 0-B undergoing curative resection. HCC tissue was collected for whole-exome sequencing, and ctDNA sequencing with an 804-gene panel was performed on peripheral blood obtained preoperatively and at 1, 4, and 7 months postoperatively to monitor MRD. The primary endpoint was recurrence-free survival. The predictive performance of MVI, longitudinal ctDNA-MRD, and their combined model was assessed using survival analysis, time-dependent receiver operating characteristic (ROC) curves, concordance index, integrated discrimination improvement, and net reclassification improvement. RESULTS: MVI-positive patients (52.0%) had poorer recurrence-free survival (hazard ratio [HR] = 3.52, p < 0.01). Longitudinal MRD status, newly emerged or persistently positive versus clearance or persistently negative, strongly predicted recurrence (hazard ratio [HR] = 16.01) and remained independently prognostic (HR = 12.45, p < 0.01). In 87.5% of cases, longitudinal MRD positivity preceded radiological recurrence (median lead time, 86 d). Time-dependent ROC analysis showed that longitudinal MRD (area under the curve [AUC] 0.785-0.849) consistently outperformed MVI (AUC 0.655-0.752) at 4, 7, 12, and 15 months postoperatively. A risk stratification model combining MVI and longitudinal MRD (low-, medium-, and high-risk) further improved predictive performance (AUC 0.853-0.899) and clinical net benefit. Integrated discrimination improvement and net reclassification improvement analyses confirmed that longitudinal MRD significantly improved predictive performance compared with MVI. CONCLUSIONS: Dynamic longitudinal ctDNA-MRD monitoring significantly outperforms static MVI assessment for recurrence prediction after HCC curative resection. This combination enables more precise postoperative risk stratification, providing insights to guide personalized adjuvant therapy decisions and follow-up strategies. TRIAL REGISTRATION: ClinicalTrials, NCT06449846. Registered 30 April 2024, https://clinicaltrials.gov/study/NCT06449846?term=NCT06449846&viewType=Card&rank=1 . CI - (c) 2026. The Author(s). FAU - Xu, Yuyan AU - Xu Y AD - Second Department of Hepatobiliary Surgery, General Surgery Center, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. FAU - Zhang, Cheng AU - Zhang C AD - Second Department of Hepatobiliary Surgery, General Surgery Center, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. FAU - Liu, Wenxuan AU - Liu W AD - Second Department of Hepatobiliary Surgery, General Surgery Center, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. FAU - Feng, Jianan AU - Feng J AD - Second Department of Hepatobiliary Surgery, General Surgery Center, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. FAU - Wang, Chunming AU - Wang C AD - Second Department of Hepatobiliary Surgery, General Surgery Center, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. FAU - He, Junming AU - He J AD - Department of Hepatobiliary Pancreatic Surgery, Guangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, 510000, China. FAU - Liang, Weichao AU - Liang W AD - Department of General Surgery, The Sixth Affiliated Hospital of South China University of Technology (Nanhai District People's Hospital), Foshan, 528000, China. FAU - Wu, Qingsong AU - Wu Q AD - Department of Hepatobiliary Surgery, Yuebei People's Hospital, Shaoguan, 512000, China. FAU - Wu, Yinbing AU - Wu Y AD - Department of Hepatobiliary Surgery, Affillated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou, 510000, China. FAU - Wang, Weidong AU - Wang W AD - Department of Hepatobiliary Surgery, The Eighth Affiliated Hospital of Southern Medical University, Foshan, 528000, China. FAU - Tan, Hongkun AU - Tan H AD - Second Department of Hepatobiliary Surgery, General Surgery Center, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. FAU - Wen, Yaohong AU - Wen Y AD - Second Department of Hepatobiliary Surgery, General Surgery Center, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. FAU - Peng, Qing AU - Peng Q AD - Central Laboratory of The Second Affiliated Hospital, School of Medicine, The Chinese University of Hong Kong, Shenzhen & Longgang District People's Hospital of Shenzhen, Shenzhen, 518000, China. FAU - Zhong, Jiasheng AU - Zhong J AD - LifeX Institute, Gannan Medical University, Ganzhou, 341000, China. FAU - Cai, Lei AU - Cai L AD - Second Department of Hepatobiliary Surgery, General Surgery Center, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. cailei_427@163.com. FAU - Pan, Mingxin AU - Pan M AUID- ORCID: 0000-0002-3979-5571 AD - Second Department of Hepatobiliary Surgery, General Surgery Center, Zhujiang Hospital, Southern Medical University, Guangzhou, 510000, China. panmx@smu.edu.cn. LA - eng SI - ClinicalTrials.gov/NCT06449846 GR - 82373159/National Natural Science Foundation of China/ GR - 2024P-GX20/Cutting-edge and Major Clinical Technology Projects in Guangzhou/ GR - 2024B03J1381/Key Technologies Research and Development Program of Guangzhou Municipality/ GR - LGKCYLWS2024-12/Shenzhen Longgang District Medical and Health Technology Key Support Project/ GR - JCYJ20250604180128037/Natural Science Foundation of Shenzhen Municipality/ GR - 2023B03J1247/Science and Technology Project in Guangzhou/ GR - 2023B1111020008/Key-Area Research and Development Program of Guangdong Province/ PT - Journal Article DEP - 20261029 PL - United States TA - Hepatol Int JT - Hepatology international JID - 101304009 SB - IM OTO - NOTNLM OT - Circulating tumor DNA (ctDNA) OT - Hepatocellular carcinoma (HCC) OT - Microvascular invasion (MVI) OT - Minimal residual disease (MRD) OT - Postoperative recurrence OT - Risk prediction COIS- Declarations. Conflict of interest: Yuyan Xu, Cheng Zhang, Wenxuan Liu, Jianan Feng, Chunming Wang, Junming He, Weichao Liang, Qingsong Wu, Yinbing Wu, Weidong Wang, Hongkun Tan, Yaohong Wen, Qing Peng, Jiasheng Zhong, Lei Cai, Mingxin Pan declare that they have no competing interests. Ethical approval and consent to participate: The study protocol received approval from the Medical Ethics Committee of Zhujiang Hospital, Southern Medical University (approval no. 2023-KY-232-01) and was carried out in compliance with the Declaration of Helsinki. All participants provided written informed consent. The trial is registered at ClinicalTrials.gov (NCT06449846) and chictr.org.cn (ChiCTR2300078077). Consent for publication: Not applicable. This manuscript does not contain any individual person's data in any form (including any individual details, images, or videos). All data are presented in an aggregate and de-identified format. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:30 PHST- 2026/05/25 00:00 [received] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:30 [entrez] AID - 10.1007/s12072-026-11163-8 [pii] AID - 10.1007/s12072-026-11163-8 [doi] PST - aheadofprint SO - Hepatol Int. 2026 Oct 29. doi: 10.1007/s12072-026-11163-8. PMID- 42955549 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1559-0755 (Electronic) IS - 0257-277X (Linking) VI - 74 IP - 1 DP - 2026 Oct 29 TI - Group A streptococcus vaccine strategies: virulence targets, resistance constraints and complementary control measures. LID - 131 [pii] LID - 10.1007/s12026-026-09865-0 [doi] AB - Group A Streptococcus (GAS), or Streptococcus pyogenes, causes a connected spectrum of pharyngeal, cutaneous, invasive and immune-mediated diseases. Although beta-lactams remain active, treatment alone has not controlled transmission, recurrent infection, rapid invasive progression or acute rheumatic fever and rheumatic heart disease. Control is further weakened by increased macrolide and clindamycin resistance, heterogeneous diagnostic access, asymptomatic carriage, crowded living conditions and unreliable continuity of secondary prophylaxis. These limitations have shifted the field from a predominantly treatment-centered approach toward the development of vaccines as a prevention strategy. Target selection is guided by virulence functions that are required for colonization, immune evasion and tissue injury. These include the M protein, group A carbohydrate (GAC), streptolysin O, Streptococcus pyogenes cell envelope protease (SpyCEP), Streptococcus pyogenes adhesion and division protein (SpyAD), C5a peptidase and other conserved surface or secreted antigens. Current strategies include multivalent type-specific M-protein vaccines, conserved M-protein peptide vaccines, glycoconjugates, multicomponent non-M-protein formulations and mRNA-lipid nanoparticle platforms. The clinical pipeline has recently broadened. Phase I studies have been completed for StreptAnova and MJ8VAX, whereas the J8-K4S2 and p*17-K4S2 clinical trial studies have been completed, with peer-reviewed results pending. The GSK four-component vaccine initiated phase I evaluation in 2025 and remained under clinical evaluation in 2026, whereas the VAX-A1 formulation entered a first-in-human phase I study in 2026. However, no licensed GAS vaccine is available. Progress remains constrained by uncertain correlates of protection, autoimmune safety requirements, geographical emm diversity, endpoint selection and the need for trials in high-burden populations. This narrative review therefore focuses on the disease problem, targetable virulence mechanisms, limits of antimicrobial control, current vaccine pipeline and interventions required to complement future vaccine use. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Agarwal, Manisha AU - Agarwal M AUID- ORCID: 0009-0000-9118-5108 AD - Centre for Biotechnology and Bioinformatics, Dibrugarh University, Dibrugarh, Assam, 786004, India. FAU - Khataniar, Ankita AU - Khataniar A AUID- ORCID: 0000-0003-3586-8267 AD - Centre for Biotechnology and Bioinformatics, Dibrugarh University, Dibrugarh, Assam, 786004, India. FAU - Rajkhowa, Sanchaita AU - Rajkhowa S AUID- ORCID: 0000-0002-4834-2654 AD - Centre for Biotechnology and Bioinformatics, Dibrugarh University, Dibrugarh, Assam, 786004, India. s_rajkhowa@dibru.ac.in. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - United States TA - Immunol Res JT - Immunologic research JID - 8611087 RN - 0 (Streptococcal Vaccines) RN - 0 (Antigens, Bacterial) RN - 0 (Protein Subunit Vaccines) RN - 0 (Carrier Proteins) SB - IM MH - Humans MH - *Streptococcal Vaccines/immunology MH - *Streptococcus pyogenes/immunology/pathogenicity MH - *Streptococcal Infections/immunology/prevention & control MH - Animals MH - Virulence MH - Antigens, Bacterial/immunology MH - Protein Subunit Vaccines MH - Drug Resistance, Bacterial MH - Vaccine Development MH - Carrier Proteins/immunology OTO - NOTNLM OT - Streptococcus pyogenes OT - Antimicrobial resistance OT - Group A carbohydrate OT - Group A streptococcus OT - M protein OT - Rheumatic heart disease OT - SpyCEP OT - Vaccine COIS- Declarations. Ethics approval and consent to participate: This study is a review article and does not involve human participants or animal subjects. Therefore, ethical approval and informed consent were not needed. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. Conflict of interest: The authors declare that they have no conflicts of interest. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:30 PHST- 2026/08/15 00:00 [received] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:30 [entrez] AID - 10.1007/s12026-026-09865-0 [pii] AID - 10.1007/s12026-026-09865-0 [doi] PST - epublish SO - Immunol Res. 2026 Oct 29;74(1):131. doi: 10.1007/s12026-026-09865-0. PMID- 42955539 OWN - NLM STAT- Publisher LR - 20261029 IS - 1863-4362 (Electronic) IS - 0021-1265 (Linking) DP - 2026 Oct 29 TI - Achieving opioid-free anaesthesia. LID - 10.1007/s11845-026-04666-8 [doi] AB - BACKGROUND: Opioids have been central to anaesthetic practice for over a century, but they risk adverse effects including postoperative nausea and vomiting (PONV), ileus, respiratory depression, and opioid-induced hyperalgesia. These complicate recovery and contribute to the wider burden of opioid misuse, a growing concern in Irish healthcare. Opioid-free anaesthesia (OFA) supports Enhanced Recovery After Surgery (ERAS) principles by removing opioid-related barriers to early mobilisation and functional recovery. AIMS: To review the rationale for OFA, how it is delivered, its limitations, and to assess how it fits into current perioperative practice in Ireland. RESULTS: OFA relies on combining agents that target multiple nociceptive pathways, such as N-methyl-D-aspartate (NMDA) receptor antagonists (ketamine, magnesium), alpha-2 adrenergic agonists (especially dexmedetomidine), non-steroidal anti-inflammatory drugs (NSAIDs), paracetamol, intravenous lidocaine, and gabapentinoids. Where applicable, ultrasound-guided peripheral nerve blocks can reduce intraoperative analgesic requirements and extending postoperative analgesia. Evidence supports OFA across a range of surgical contexts. Benefits are most consistent in laparoscopic and major abdominal surgery, where faster return of bowel function, reduced length of stay, and lower rates of PONV are reported. Evidence on postoperative analgesia is less clear. Opioid consumption in the immediate post-anaesthetic period tends to be lower but is often comparable to a well-executed opioid-inclusive multimodal regimen thereafter. CONCLUSIONS: Rigid opioid avoidance risks undertreating acute pain, so OFA is best applied selectively, guided by procedure type, patient comorbidities, and institutional resources. This review evaluates the rationale, implementation, and limitations of OFA, providing clinicians with a balanced appraisal of its role in modern perioperative care. CI - (c) 2026. The Author(s), under exclusive licence to Royal Academy of Medicine in Ireland. FAU - Hannon, David M AU - Hannon DM AUID- ORCID: 0000-0001-6593-2085 AD - Department of Anaesthesia and Critical Care, National Neuroscience Centre, Beaumont Hospital, Dublin, Ireland. davidmhannon@gmail.com. FAU - Moore, Michael AU - Moore M AD - Department of Anaesthesia and Critical Care, National Neuroscience Centre, Beaumont Hospital, Dublin, Ireland. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - Ireland TA - Ir J Med Sci JT - Irish journal of medical science JID - 7806864 SB - IM OTO - NOTNLM OT - Dexmedetomidine OT - ERAS OT - Hyperalgesia OT - Multimodal analgesia OT - Opioid-free anaesthesia OT - PONV COIS- Declarations. Ethics approval and consent to participate: Not applicable. Conflict of interest: The authors declare no conflict of interest. Declaration of AI and AI-assisted technologies in the writing process: AI tools were not used as part of the writing process for this work. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:29 PHST- 2026/07/09 00:00 [received] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:29 [entrez] AID - 10.1007/s11845-026-04666-8 [pii] AID - 10.1007/s11845-026-04666-8 [doi] PST - aheadofprint SO - Ir J Med Sci. 2026 Oct 29. doi: 10.1007/s11845-026-04666-8. PMID- 42955535 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1534-3170 (Electronic) IS - 1523-3782 (Linking) VI - 28 IP - 1 DP - 2026 Oct 29 TI - Are Injectable Antihypertensives the Answer to Improving Blood Pressure Control: SiRNAs and Antisense Oligonucleotide Agents. LID - 120 [pii] LID - 10.1007/s11886-026-02440-x [doi] AB - PURPOSE OF REVIEW: Hypertension remains the leading modifiable risk factor for cardiovascular morbidity and mortality worldwide, yet blood pressure control rates remain suboptimal despite the widespread availability of effective antihypertensive therapies. Poor long-term adherence, therapeutic inertia, and the need for lifelong daily treatment continue to limit the effectiveness of current pharmacological strategies. This review examines the biological rationale, available clinical evidence, and potential role of long-acting RNA-based therapies targeting hepatic angiotensinogen synthesis in hypertension. RECENT FINDINGS: Small interfering RNAs (siRNAs) and antisense oligonucleotides (ASOs) targeting angiotensinogen represent a novel therapeutic approach that suppresses production of the unique precursor of the renin-angiotensin system. Zilebesiran has demonstrated sustained reductions in circulating angiotensinogen and ambulatory blood pressure following infrequent subcutaneous administration in phase 1 and phase 2 clinical trials. Antisense strategies have provided additional clinical validation of sustained angiotensinogen inhibition, although their antihypertensive efficacy has been less consistent. Ongoing clinical development is addressing long-term cardiovascular outcomes, safety, and optimal patient selection. RNA-based antihypertensive therapies may represent an important evolution in hypertension management by combining upstream renin-angiotensin system inhibition with prolonged pharmacological activity and reduced dependence on daily medication adherence. However, their future clinical role will depend on evidence regarding long-term safety, cardiovascular outcomes, reversibility, cost-effectiveness, patient selection, and integration with conventional antihypertensive therapy. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Sabouret, Pierre AU - Sabouret P AD - ACTION Study Group, Heart Institute, Sorbonne University, Inserm UMRS1166, Pitie-Salpetriere Hospital, 47-83 Bd de L'Hopital, Paris, 75013, France. cardiology.sabouret@gmail.com. FAU - Giamundo, Domenico Mario AU - Giamundo DM AD - Division of Cardiology, Policlinico Casilino, Rome, Italy. FAU - Ibrahim, Ahmed AU - Ibrahim A AD - Faculty of Medicine, Alexandria University, Alexandria, Egypt. FAU - Calabro, Paolo AU - Calabro P AD - Department of Translational Medical Sciences, University of Campania Luigi Vanvitelli, Caserta, Italy. AD - Division of Clinical Cardiology, A.O.R.N. Sant'Anna e San Sebastiano, Caserta, Italy. FAU - Ando, Giuseppe AU - Ando G AD - Department of Clinical and Experimental Medicine, University of Messina, Messina, Italy. FAU - Pathak, Mina AU - Pathak M AD - INSERM U 1070, Toulouse, France. FAU - Atar, Dan AU - Atar D AD - Department of Cardiology, Oslo University Hospital, Oslo, Norway. AD - Institute of Clinical Medicine, University of Oslo, Oslo, Norway. FAU - Pathak, Atul AU - Pathak A AD - National Heart Hospital, Luxembourg City, Luxembourg. AD - INSERM U 1070, Toulouse, France. AD - Princess Grace ESH Excellence Center, Monte Carlo, Monaco. AD - University of Luxembourg, Luxembourg City, Luxembourg. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - United States TA - Curr Cardiol Rep JT - Current cardiology reports JID - 100888969 RN - 0 (Oligonucleotides, Antisense) RN - 0 (Antihypertensive Agents) RN - 0 (RNA, Small Interfering) RN - 11002-13-4 (Angiotensinogen) SB - IM MH - Humans MH - *Hypertension/drug therapy/physiopathology MH - *Oligonucleotides, Antisense/therapeutic use/administration & dosage MH - *Antihypertensive Agents/therapeutic use/administration & dosage MH - *RNA, Small Interfering/therapeutic use/administration & dosage MH - Angiotensinogen/genetics MH - Renin-Angiotensin System/drug effects MH - Blood Pressure/drug effects OTO - NOTNLM OT - Angiotensinogen OT - Antisense oligonucleotides OT - Hypertension OT - Renin-angiotensin-aldosterone system OT - Small interfering RNA OT - Zilebesiran COIS- Declarations. Human and Animal Rights and Informed Consent: This article does not contain any studies with human or animal subjects performed by any of the authors. Use of Artificial Intelligence: Generative artificial intelligence tools were used during manuscript preparation to assist with language editing, drafting, and improvement of clarity and readability. All AI-assisted content was critically reviewed, verified, and revised by the authors. Artificial intelligence was not used for data generation or analysis, and the authors take full responsibility for the content of the manuscript. Conflict of interest: The authors declare that they have no conflict of interest. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:29 PHST- 2026/09/12 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:29 [entrez] AID - 10.1007/s11886-026-02440-x [pii] AID - 10.1007/s11886-026-02440-x [doi] PST - epublish SO - Curr Cardiol Rep. 2026 Oct 29;28(1):120. doi: 10.1007/s11886-026-02440-x. PMID- 42955534 OWN - NLM STAT- Publisher LR - 20261029 IS - 1618-1905 (Electronic) IS - 1139-6709 (Linking) DP - 2026 Oct 29 TI - Candidozyma auris in intensive care units: A point prevalence study and six-month colonization-infection surveillance. LID - 10.1007/s10123-026-00902-4 [doi] AB - BACKGROUND: Candidozyma auris has emerged as a globally significant nosocomial pathogen due to its multidrug antifungal resistance, environmental persistence, and high mortality rates. This study aimed to determine the prevalence of C. auris colonization through a point prevalence screening in the intensive care units (ICUs) of our hospital and to evaluate colonization and infection outcomes during a subsequent six-month follow-up period. OBJECTIVES: The present study aimed to evaluate C. auris colonization rates through point prevalence screening in the ICUs of our hospital, to assess colonization and infection outcomes during a subsequent six-month follow-up period, and to establish a diagnostic algorithm for C. auris identification in our laboratory. METHODS: Following the detection of an index C. auris case in our hospital in February 2025, a point prevalence study was conducted in all ICUs. Composite swab samples (bilateral axilla and groin) were obtained from hospitalized patients on the same day. In addition, urine and wound cultures were collected from eligible patients. Isolates were identified using chromogenic media, conventional microbiological methods, and MALDI-TOF MS. After the point prevalence study, surveillance for colonization and bloodstream infection was performed over a six-month period. RESULTS: C. auris colonization was detected in 7 of 70 ICU patients (10%) based on composite swab samples. During the six-month follow-up, colonization was identified in 15 patients, and C. auris fungemia developed in nine patients. All patients with positive blood cultures died. The most frequently identified risk factors were broad-spectrum antibiotic use, prolonged ICU stay, and the presence of urinary catheters. CONCLUSION: Our findings demonstrate that C. auris colonization can be detected at a high rate in ICUs and that severe invasive infections may develop following colonization. Early diagnosis, active screening strategies, and effective infection control measures are critical for reducing C. auris-associated morbidity and mortality. CI - (c) 2026. The Author(s). FAU - Yis, Reyhan AU - Yis R AD - Department of Medical Microbiology, Bakircay University of Medical School, Izmir, Turkey. reyhan.yis@bakircay.edu.tr. FAU - Senol, Gunes AU - Senol G AD - Department of Infectious Diseases, Bakircay University of Medical School, Izmir, Turkey. LA - eng PT - Journal Article DEP - 20261029 PL - Switzerland TA - Int Microbiol JT - International microbiology : the official journal of the Spanish Society for Microbiology JID - 9816585 SB - IM OTO - NOTNLM OT - Candida auris OT - Candidozyma auris OT - Bloodstream Infection OT - Colonization OT - Infection Control OT - Intensive Care Units COIS- Declarations. Ethics approval and consent to participate: This study was approved by the Izmir Bakircay University Clinical Research Ethics Committee (Approval No: 2732, Date: 28.01.2026). The requirement for informed consent was waived due to the retrospective observational design of the study. Human Ethics and Consent to Participate: This study was approved by the Izmir Bakircay University Clinical Research Ethics Committee (Approval No: 2732, Date: 28.01.2026). The requirement for informed consent was waived due to the retrospective observational design of the study. Conflict of interest: The authors declare that they have no conflict of interest. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:29 PHST- 2026/02/11 00:00 [received] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/05/17 00:00 [revised] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:29 [entrez] AID - 10.1007/s10123-026-00902-4 [pii] AID - 10.1007/s10123-026-00902-4 [doi] PST - aheadofprint SO - Int Microbiol. 2026 Oct 29. doi: 10.1007/s10123-026-00902-4. PMID- 42955533 OWN - NLM STAT- Publisher LR - 20261029 IS - 1573-4919 (Electronic) IS - 0300-8177 (Linking) DP - 2026 Oct 29 TI - QSOX1 as a mediator of tumor-fibroblast crosstalk and a modulator of pulmonary tumor burden in lung adenocarcinoma. LID - 10.1007/s11010-026-05786-6 [doi] AB - Quiescin sulfhydryl oxidase 1 (QSOX1), an enzyme that catalyzes disulfide bond formation, is expressed in various cancers, but its role in the tumor microenvironment (TME) remains unclear. Here, we investigated the role of QSOX1 in tumor-stromal interactions in lung adenocarcinoma (LUAD). Analysis of public datasets revealed elevated QSOX1 expression in early-stage LUAD and its association with poor prognosis. Given the central role of cancer-associated fibroblasts (CAFs) in extracellular matrix (ECM) organization and tumor progression, we examined whether QSOX1 expression in LUAD cells affects fibroblast activation. Modulation of QSOX1 expression in lung cancer cells altered CAF-associated marker expression, ECM deposition, collagen gel contraction, and fibroblast migration, accompanied by changes in YAP/TAZ and integrin beta1/alphaV expression. QSOX1 was also expressed in stromal cells in normal lung tissue and was predominantly detected in fibroblasts. We therefore examined whether fibroblast QSOX1 affects tumor cell behavior and found that fibroblast QSOX1 influenced tumor cell migration and invasion in vitro. In vivo, QSOX1 deficiency was associated with reduced pulmonary tumor burden. Collectively, our findings suggest that QSOX1 acts as a mediator of lung cancer-fibroblast crosstalk and a modulator of pulmonary tumor burden in early-stage LUAD. CI - (c) 2026. The Author(s). FAU - Lee, Seo-Yeon AU - Lee SY AD - Department of Biochemistry, Brain Korea 21 Project and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, 08826, Republic of Korea. AD - Comparative Medicine Disease Research Center (CDRC), Science Research Center (SRC), Seoul National University, Seoul, 08826, Republic of Korea. AD - Department of Biochemistry, College of Veterinary Medicine, Seoul National University, Gwanak-ro1, Gwanak-gu, Seoul, Korea. FAU - Yoon, Sang Hyuk AU - Yoon SH AD - Department of Biochemistry, Brain Korea 21 Project and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, 08826, Republic of Korea. FAU - Yoon, Won-Kee AU - Yoon WK AD - Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, Republic of Korea. FAU - Kim, Chae-Yi AU - Kim CY AD - Comparative Medicine Disease Research Center (CDRC), Science Research Center (SRC), Seoul National University, Seoul, 08826, Republic of Korea. FAU - Cho, Je-Yoel AU - Cho JY AUID- ORCID: 0000-0003-1030-3577 AD - Department of Biochemistry, Brain Korea 21 Project and Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, 08826, Republic of Korea. jeycho@snu.ac.kr. AD - Comparative Medicine Disease Research Center (CDRC), Science Research Center (SRC), Seoul National University, Seoul, 08826, Republic of Korea. jeycho@snu.ac.kr. AD - Department of Biochemistry, College of Veterinary Medicine, Seoul National University, Gwanak-ro1, Gwanak-gu, Seoul, Korea. jeycho@snu.ac.kr. LA - eng GR - RS-2021-NR060088/Ministry of Science and ICT and the National Research Foundation of Korea (NRF) SRC program/ GR - RS-2021-NR060088/Ministry of Science and ICT and the National Research Foundation of Korea (NRF) SRC program/ GR - RS-2021-NR060088/Ministry of Science and ICT and the National Research Foundation of Korea (NRF) SRC program/ PT - Journal Article DEP - 20261029 PL - Netherlands TA - Mol Cell Biochem JT - Molecular and cellular biochemistry JID - 0364456 SB - IM OTO - NOTNLM OT - Cancer-associated fibroblast (CAF) OT - Extracellular matrix (ECM) OT - Lung adenocarcinoma (LUAD) OT - Quiescin sulfhydryl oxidase 1 (QSOX1) OT - Tumor microenvironment (TME) COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:29 PHST- 2026/03/30 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:29 [entrez] AID - 10.1007/s11010-026-05786-6 [pii] AID - 10.1007/s11010-026-05786-6 [doi] PST - aheadofprint SO - Mol Cell Biochem. 2026 Oct 29. doi: 10.1007/s11010-026-05786-6. PMID- 42955531 OWN - NLM STAT- Publisher LR - 20261029 IS - 1573-4919 (Electronic) IS - 0300-8177 (Linking) DP - 2026 Oct 28 TI - Trehalose alleviates oxidative stress-induced senescence and is associated with cell-cycle remodeling. LID - 10.1007/s11010-026-05788-4 [doi] AB - Trehalose has been widely used as a food and drug additive for a long time, and its effects on antioxidant stress, autophagy, and anti-aging have been gradually studied. However, the molecular mechanism by which trehalose exerts anti-oxidative stress and anti-aging effects has not been fully clarified. Our anti-aging cell phenotype study based on trehalose validates its intrinsic mechanisms of anti-oxidative stress and anti-inflammation in cells. We analyzed publicly available transcriptomic data (GSE76643) to identify molecular pathways regulated by trehalose, and then validated the key findings in our own cellular and animal experiments. The results indicate that trehalose alleviates oxidative stress-induced senescence, and that this effect is associated with remodeling of cell cycle progression, including down-regulation of selected cell-cycle regulators. The D-Gal-induced premature aging mouse model demonstrated that trehalose can effectively alleviate aging phenotypes in the hair, liver metabolic abnormalities, lung, and kidney of mice. Trehalose treatment reversed the D‑galactose‑induced upregulation of P16, P21, and P53 proteins in the liver, lung, and kidney, further supporting its anti‑aging effects in vivo. Additionally, the down-regulated expression of BIRC5, CCNE2, and CDK2 mRNA in certain tissues suggests that trehalose regulates or intervenes in the cell cycle. Based on the cell-cycle remodeling phenotype, we hypothesized that trehalose may modulate P65, STAT3, Keap1-Kelch, and CDK2. Molecular docking predicted potential interactions between trehalose and these proteins; these predictions require experimental validation. In conclusion, this study provides evidence that trehalose may alleviate oxidative stress-induced senescence in association with cell-cycle remodeling. These findings lay a foundation for further development of trehalose as an anti-aging intervention, but direct causality remains to be established. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Yang, Yu AU - Yang Y AD - Laboratory of Aging and Geriatric Medicine, National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China. FAU - Chen, Honghan AU - Chen H AD - Laboratory of Aging and Geriatric Medicine, National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China. FAU - Yang, Ming AU - Yang M AD - Laboratory of Aging and Geriatric Medicine, National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China. FAU - Li, Huan AU - Li H AD - Institute of Anesthesiology Research, West China Hospital, Sichuan University, Chengdu, China. FAU - Xu, Weitong AU - Xu W AD - Laboratory of Aging and Geriatric Medicine, National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China. FAU - Gong, Hui AU - Gong H AD - Laboratory of Aging and Geriatric Medicine, National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China. FAU - Yang, Yang AU - Yang Y AD - Animal Experimental Center of West China Hospital, Sichuan University, Chengdu, China. FAU - Mo, Yuanliang AU - Mo Y AD - Laboratory of Aging and Geriatric Medicine, National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China. FAU - Huang, Ning AU - Huang N AD - Laboratory of Aging and Geriatric Medicine, National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China. FAU - Liu, Jin AU - Liu J AD - Laboratory of Aging and Geriatric Medicine, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, China. FAU - Huang, Xiaoli AU - Huang X AD - The Center of Gerontology and Geriatrics, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, China. FAU - Xiao, Hengyi AU - Xiao H AD - Laboratory of Aging and Geriatric Medicine, National Clinical Research Center for Geriatrics, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China. hengyix@scu.edu.cn. LA - eng GR - 2018YFC2000400/National Key Research and Development Program of China/ GR - 82071589/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261028 PL - Netherlands TA - Mol Cell Biochem JT - Molecular and cellular biochemistry JID - 0364456 SB - IM OTO - NOTNLM OT - Anti-aging OT - Antioxidant OT - Cell cycle remodeling OT - Oxidative stress OT - Trehalose COIS- Declarations. Ethics approval: The studies involving experimental animal were reviewed and approved by The Institutional Research Ethics Committee of West China Hospital, Sichuan University. Consent for publication: All data and samples involved in this study have been obtained with their knowledge and permission for publication. Conflict of interests: The authors declare no conflict of interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:29 PHST- 2026/04/23 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:29 [entrez] AID - 10.1007/s11010-026-05788-4 [pii] AID - 10.1007/s11010-026-05788-4 [doi] PST - aheadofprint SO - Mol Cell Biochem. 2026 Oct 28. doi: 10.1007/s11010-026-05788-4. PMID- 42955524 OWN - NLM STAT- Publisher LR - 20261029 IS - 1573-6628 (Electronic) IS - 1092-7875 (Linking) DP - 2026 Oct 28 TI - Effects of Mobile-Based Multicomponent Infant Sleep Intervention on Infants and Mothers: A Randomized Controlled Trial. LID - 10.1007/s10995-026-04335-3 [doi] AB - OBJECTIVES: This study evaluated the effects of a mobile-based multicomponent infant sleep intervention on infant and maternal sleep and maternal mental health. METHODS: In this single-blind randomized controlled trial, mother-infant dyads with infants aged 3-12 months were recruited from an online parenting community in South Korea; 32 dyads were randomized and 29 were analyzed. From February to May 2021, the intervention group used a mobile application for 4 weeks providing infant sleep education, sleep monitoring, and personalized support. Sleep was assessed by actigraphy for 5 consecutive days at baseline, post-intervention (6 weeks), and follow-up (10 weeks). Maternal depression, fatigue, and sleep disturbance were assessed using the Patient-Reported Outcomes Measurement Information System. Linear mixed-effects models examined group differences over time. RESULTS: Compared with the control group, infants in the intervention group showed greater improvements from baseline in sleep efficiency and wake after sleep onset at 6 and 10 weeks and in average awake time at 10 weeks. Mothers in the intervention group showed greater improvements in sleep efficiency, wake after sleep onset, and number of awakenings at 10 weeks. They also had a greater decrease in depression scores at 10 weeks and a difference in sleep disturbance scores favoring the intervention at 6 weeks, whereas fatigue did not differ between groups. CONCLUSIONS FOR PRACTICE: A mobile-based, family-centered multicomponent sleep intervention was associated with greater improvements in selected infant and maternal sleep outcomes and maternal depression scores. Future studies should evaluate the long-term effectiveness and stability of these effects. CLINICAL TRIAL REGISTRATION: This study was registered with the Clinical Research Information Service, a member of the World Health Organization International Clinical Trials Registry Platform (KCT0005283), registered on August 4, 2020 ( https://cris.nih.go.kr/cris/search/detailSearch.do?seq=22693&search_page=L ). CI - (c) 2026. The Author(s). FAU - Chae, Sun-Mi AU - Chae SM AUID- ORCID: 0000-0002-3010-2265 AD - College of Nursing, The Research Institute of Nursing Science, Seoul National University, Seoul, Republic of Korea. FAU - Kim, Cho Hee AU - Kim CH AUID- ORCID: 0000-0001-9388-2126 AD - College of Nursing, Kangwon National University, 1, Gangwondaehak-gil, Chuncheon, Republic of Korea. chkim@kangwon.ac.kr. FAU - Kim, Mi-Ra AU - Kim MR AUID- ORCID: 0000-0003-4298-0310 AD - Department of Bio & Medical Big Data (BK4 Program), Department of Information Statistics, Gyeongsang National University, Jinju, Republic of Korea. FAU - Chung, Na-Ry AU - Chung NR AUID- ORCID: 0000-0002-2741-4890 AD - College of Nursing, Seoul National University, Seoul, Republic of Korea. FAU - Yeo, Ji-Young AU - Yeo JY AUID- ORCID: 0000-0001-6375-4668 AD - Institute for Artificial Intelligence Research, Hanyang University, 222, Wangsimni-ro, Seongdong-gu, Seoul, Republic of Korea. shine73@hanyang.ac.kr. FAU - Park, Chang Gi AU - Park CG AUID- ORCID: 0000-0003-3366-4279 AD - College of Nursing, University of Illinois Chicago, Chicago, IL, USA. LA - eng GR - 2018R1A2B6005201/Ministry of Science and ICT, South Korea/ PT - Journal Article DEP - 20261028 PL - United States TA - Matern Child Health J JT - Maternal and child health journal JID - 9715672 SB - IM OTO - NOTNLM OT - Infant OT - Mobile application OT - Mother OT - Randomized controlled trial OT - Sleep COIS- Declarations. Ethics approval and Consent to participate: The study protocol was approved by the Institutional Review Board of the authors' institution (IRB No. 1806/001-002). Written informed consent was obtained from all participating mothers for their own participation and on behalf of their infants. Consent for Publication: Not applicable. Conflict of interest: The authors declare no conflicts of interest. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:29 PHST- 2025/10/09 00:00 [received] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:29 [entrez] AID - 10.1007/s10995-026-04335-3 [pii] AID - 10.1007/s10995-026-04335-3 [doi] PST - aheadofprint SO - Matern Child Health J. 2026 Oct 28. doi: 10.1007/s10995-026-04335-3. PMID- 42955518 OWN - NLM STAT- Publisher LR - 20261029 IS - 1534-4681 (Electronic) IS - 1068-9265 (Linking) DP - 2026 Oct 28 TI - Society of Surgical Oncology Best Practice Statement on the Implementation of New Programs and Technologies Into Practice: An IDEAL-Informed Framework. LID - 10.1245/s10434-026-20767-1 [doi] AB - Innovation in surgical oncology is developing rapidly, and new therapies and technologies have expanded across academic centers, integrated networks, and community sites, at times outpacing the evidence, expertise, and necessary infrastructure. Although innovation is essential to our field's progress, patient safety and demonstrated efficacy must remain paramount. Adopting specialized or understudied approaches without appropriate guardrails risks patient harm and generates low-quality, confusing evidence that impedes progress for future patients with cancer. To provide structured, practical guidance on this challenge to our field, the Society of Surgical Oncology Quality Committee recommends application of the IDEAL framework for surgical innovation, which organizes the life cycle of new procedures, programs, and technologies into five stages: idea (stage 1), development (stage 2a), exploration (stage 2b), assessment (stage 3), and long-term study (stage 4). Using expert consensus, we illustrate this IDEAL-informed approach with four examples that represent the breadth of the current implementation landscape in surgical oncology: hepatic artery infusion pump chemotherapy, robotic nipple-sparing mastectomy, histotripsy, and artificial intelligence. These technologies are used as concrete illustrations of principle, not as the primary subject of this statement. The central tenant is this: before any program or technology is broadly taken to the bedside, there must be evidence to support its efficacy. The recommendations described in this report have been endorsed by the Society of Surgical Oncology. CI - (c) 2026. Society of Surgical Oncology. FAU - Ocuin, Lee M AU - Ocuin LM AUID- ORCID: 0000-0003-3341-9521 AD - Division of Surgical Oncology, Jefferson-Lehigh Valley Topper Cancer Institute, Allentown, PA, USA. lee.ocuin@jefferson.edu. FAU - Hwang, Rosa F AU - Hwang RF AD - Department of Breast Surgical Oncology, MD Anderson Cancer Center, Houston, TX, USA. FAU - Park, Ko Un AU - Park KU AD - Department of Surgery, Division of Breast Surgical Oncology, Brigham & Women's Hospital, Boston, MA, USA. FAU - Farr, Deborah AU - Farr D AD - Department of Surgery, Division of Surgical Oncology, UT Southwestern, Dallas, TX, USA. FAU - Hashimoto, Daniel AU - Hashimoto D AD - Department of Surgery, Division of Minimally Invasive Surgery, Duke University Medical Center, Durham, NC, USA. FAU - Thiels, Cornelius AU - Thiels C AD - Department of Surgery, Division of Hepatopancreatobiliary Surgery, Mayo Clinic, Rochester, MN, USA. FAU - Cho, Clifford AU - Cho C AD - Department of Surgery, Division of Hepatopancreatobiliary and Advanced GI Surgery, University of Michigan Medical Center, Ann Arbor, MI, USA. FAU - Gholami, Sepideh AU - Gholami S AD - Department of Surgery, Division of Surgical Oncology, Northwell Health, Lake Success, NY, USA. FAU - Groot Koerkamp, Bas AU - Groot Koerkamp B AD - Department of Surgery, Erasmus MC, Rotterdam, The Netherlands. FAU - Mayo, Skye C AU - Mayo SC AD - Department of Surgery, Division of Surgical Oncology, Knight Cancer Institute at Oregon Health & Science University (OHSU), Portland, OR, USA. FAU - Lidsky, Michael E AU - Lidsky ME AD - Department of Surgery, Division of Surgical Oncology, Duke University Medical Center, Durham, NC, USA. FAU - D'Angelica, Michael AU - D'Angelica M AD - Department of Surgery, Section of Hepatopancreratobiliary Surgery, Memorial Sloan-Kettering Cancer Center, New York, NY, USA. FAU - Katz, Matthew H G AU - Katz MHG AD - Department of Surgical Oncology, MD Anderson Cancer Center, Houston, TX, USA. FAU - Moo, Tracy-Ann AU - Moo TA AD - Department of Surgery, Section of Breast Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, USA. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Ann Surg Oncol JT - Annals of surgical oncology JID - 9420840 SB - IM OTO - NOTNLM OT - Artificial intelligence OT - Best practices OT - Hepatic artery infusion pump therapy OT - Histotripsy OT - IDEAL framework OT - Practice implementation OT - Robotic nipple-sparing mastectomy COIS- Disclosure: AI tools were used to assist with specific aspects of manuscript preparation. ChatGPT 5.2 (OpenAI), Copilot (Microsoft), Claude (Anthropic), and OpenEvidence were used for literature search, word choice refinement, and editing. The authors take full responsibility for the accuracy, integrity, and content of this publication. Clifford Cho owns intellectual property licensed through the University of Michigan and Ann Arbor VA Hospital to HistoSonics, Inc. Daniel Hashimoto is a consultant for Medtronic. Lee Ocuin is a consultant for Boston Scientific. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:28 PHST- 2026/07/07 00:00 [received] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:28 [entrez] AID - 10.1245/s10434-026-20767-1 [pii] AID - 10.1245/s10434-026-20767-1 [doi] PST - aheadofprint SO - Ann Surg Oncol. 2026 Oct 28. doi: 10.1245/s10434-026-20767-1. PMID- 42955517 OWN - NLM STAT- Publisher LR - 20261029 IS - 1534-4681 (Electronic) IS - 1068-9265 (Linking) DP - 2026 Oct 28 TI - ASO Author Reflections: Beyond the Index Lymph Node: Refining Patient Selection in the De-escalation of Melanoma Surgery. LID - 10.1245/s10434-026-20805-y [doi] FAU - Goodijk, Marieke T AU - Goodijk MT AD - Department of Surgical Oncology, Netherlands Cancer Institute, Amsterdam, The Netherlands. FAU - Kuijpers, Anke M J AU - Kuijpers AMJ AD - Department of Surgical Oncology, Netherlands Cancer Institute, Amsterdam, The Netherlands. FAU - van Akkooi, Alexander C J AU - van Akkooi ACJ AD - Melanoma Institute Australia, The University of Sydney, Sydney, Australia. AD - Faculty of Medicine and Health, The University of Sydney, Sydney, Australia. AD - Department of Melanoma and Surgical Oncology, Institute of Academic Surgery, Royal Prince Alfred Hospital, Sydney, Australia. FAU - van Houdt, Winan J AU - van Houdt WJ AD - Department of Surgical Oncology, Netherlands Cancer Institute, Amsterdam, The Netherlands. w.v.houdt@nki.nl. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Ann Surg Oncol JT - Annals of surgical oncology JID - 9420840 SB - IM EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:28 PHST- 2026/10/06 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:28 [entrez] AID - 10.1245/s10434-026-20805-y [pii] AID - 10.1245/s10434-026-20805-y [doi] PST - aheadofprint SO - Ann Surg Oncol. 2026 Oct 28. doi: 10.1245/s10434-026-20805-y. PMID- 42955512 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1863-2491 (Electronic) IS - 1863-2483 (Linking) VI - 20 IP - 1 DP - 2026 Oct 29 TI - Indocyanine green near-infrared fluorescence in robotic ureteral and ureteroenteric reconstruction: from ureteral localization to perfusion-guided decision-making. LID - 1154 [pii] LID - 10.1007/s11701-026-04118-6 [doi] AB - In robot-assisted ureteral and uretero-intestinal reconstruction surgery, indocyanine green (ICG) fluorescence technology has seen increasing use to supplement traditional visual assessment. This narrative review explores the evolution of this technology from an anatomical navigation tool to a potential tool for perfusion-guided intraoperative decision-making. A structured literature search was conducted in the PubMed/MEDLINE, Embase and Web of Science databases, focusing on robot-assisted reconstruction applications, comparative outcome studies, and quantitative fluorescence methods. Intraluminal ICG enhances luminal contrast, aiding in the identification of the ureter and the localisation of strictures, particularly in reoperative or fibrotic areas. In contrast, intravenous ICG enables real-time assessment of relative tissue perfusion and may influence the selection of ureteral resection margins and anastomotic sites. The results of comparative studies evaluating the efficacy of preventing ureterointestinal anastomotic strictures have been inconsistent and remain limited by factors such as observational study designs, inconsistent fluorescence protocols, varying follow-up periods, and temporal confounding factors. Consequently, current evidence more strongly supports intraoperative utility and decision-making than consistent long-term outcome improvement. Quantitative time-intensity analysis, automated segmentation, and AI-based video analysis may reduce the subjectivity of fluorescent image interpretation; however, there are currently no validated thresholds for in situ ureteral reconstruction. Future research should standardise image acquisition, prospectively document fluorescence-guided changes in surgical strategy, and validate quantitative perfusion indices against clinically meaningful reconstruction failure. Currently, ICG should be used to complement established reconstruction principles, rather than to replace the surgeon's judgement. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature. FAU - Zhang, Chi AU - Zhang C AD - Department of Urology, The First Hospital of Jilin University, Changchun, 130021, China. FAU - Yang, Z N AU - Yang ZN AD - Anaesthesiology, Medical College of Yanbian University, Yanji, Jilin, China. FAU - Qiao, Yan AU - Qiao Y AD - Department of Anesthesiology and Operating Room, Baoji Traditional Chinese Medicine Hospital, Baoji, Shaanxi Province, China. FAU - Wu, Hao AU - Wu H AD - Department of Urology, The First Hospital of Jilin University, Changchun, 130021, China. FAU - Zhang, W Q AU - Zhang WQ AD - Department of Urology, The First Hospital of Jilin University, Changchun, 130021, China. FAU - Gao, B S AU - Gao BS AD - Department of Urology, The First Hospital of Jilin University, Changchun, 130021, China. gaobs@jlu.edu.cn. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - J Robot Surg JT - Journal of robotic surgery JID - 101300401 RN - IX6J1063HV (Indocyanine Green) SB - IM MH - *Indocyanine Green MH - Humans MH - *Ureter/surgery/diagnostic imaging MH - *Robotic Surgical Procedures/methods MH - *Plastic Surgery Procedures/methods MH - Anastomosis, Surgical/methods MH - Fluorescence MH - Ileum/surgery MH - *Optical Imaging/methods MH - Perfusion/methods OTO - NOTNLM OT - Indocyanine green OT - Near-infrared fluorescence OT - Perfusion OT - Robotic surgery OT - Ureteral reconstruction OT - Ureteroenteric anastomotic stricture COIS- Declarations. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. Ethics Approval: Not applicable. This article is a narrative review of published literature and reports no new studies involving human participants, animals, or identifiable individual data. Consent to Participate: Not applicable. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:28 PHST- 2026/09/29 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:28 [entrez] AID - 10.1007/s11701-026-04118-6 [pii] AID - 10.1007/s11701-026-04118-6 [doi] PST - epublish SO - J Robot Surg. 2026 Oct 29;20(1):1154. doi: 10.1007/s11701-026-04118-6. PMID- 42955511 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2191-1991 (Print) IS - 2191-1991 (Linking) VI - 16 IP - 1 DP - 2026 Aug 1 TI - Which stage of pharmaceutical procurement is perceived to be most vulnerable to corruption in public tertiary hospitals in Enugu State, Nigeria? LID - 128 [pii] LID - 10.1186/s13561-026-00837-6 [doi] AB - BACKGROUND: Pharmaceutical procurement in public tertiary hospitals is a critical component of healthcare delivery but may be vulnerable to corruption because it involves large financial flows, complex technical decisions and multiple actors. In Nigeria, corruption in health sector hinders access to essential medicines, drives up costs, increases circulation of falsified products and compromises patient care. This study examined which procurement stages staff involved in pharmaceutical procurement in two public tertiary hospitals in Enugu State perceived as most vulnerable to corruption. METHODS: A cross-sectional quantitative study was conducted in two public tertiary hospitals in Enugu State, Nigeria. Data were collected from 122 hospital staff directly or indirectly involved in pharmaceutical procurement. Trained research assistants administered the structured questionnaire face-to-face and recorded participants' responses. Descriptive statistics summarised respondents' characteristics and the proportions identifying each procurement stage as vulnerable. Exploratory binary logistic regression examined associations between reported indicators of potentially problematic procurement practices and perceived vulnerability at different procurement stages. RESULTS: Supplier selection was the stage most frequently perceived as vulnerable, identified by 50.8% of respondents, followed by bidding (22.1%). Logistic regression analysis confirmed significant associations between corruption and both supplier selection (OR = 6.65, p = 0.007) and the bidding process (OR = 3.74, p = 0.037). Other stages, such as drug distribution and tender advertisement, showed lower incidences and weaker associations. CONCLUSION: In this perception survey of staff in two public tertiary hospitals, supplier selection and bidding were most frequently identified as vulnerable stages. The findings identify perceived vulnerabilities, not verified instances or the prevalence of corruption. They indicate priorities for further investigation, triangulation with procurement documents and qualitative evidence, possible governance strengthening, adoption of e-procurement systems and adherence to international procurement ethical standards. CI - (c) 2026. The Author(s). FAU - Ogbozor, Pamela Adaobi AU - Ogbozor PA AUID- ORCID: 0000-0003-1343-1822 AD - Health Policy Research Group, College of Medicine, University of Nigeria Enugu Campus, Enugu, Nigeria. pamela.ogbozor@esut.edu.ng. AD - Department of Psychology, Enugu State University of Science and Technology, Enugu, Nigeria. pamela.ogbozor@esut.edu.ng. FAU - McKee, Martin AU - McKee M AD - London School of Hygiene and Tropical Medicine, London, UK. FAU - Obodoechi, Divine N AU - Obodoechi DN AD - Health Policy Research Group, College of Medicine, University of Nigeria Enugu Campus, Enugu, Nigeria. AD - Department of Economics, University of Nigeria, Nsukka, Nigeria. FAU - Balabanova, Dina AU - Balabanova D AD - London School of Hygiene and Tropical Medicine, London, UK. FAU - Onwujekwe, Obinna AU - Onwujekwe O AD - Health Policy Research Group, College of Medicine, University of Nigeria Enugu Campus, Enugu, Nigeria. AD - Department of Health Administration and Management, College of Medicine, University of Nigeria Enugu Campus, Enugu, Nigeria. LA - eng GR - Contract P07073/UK aid from the UK Government/ PT - Journal Article DEP - 20260801 PL - Germany TA - Health Econ Rev JT - Health economics review JID - 101583209 OTO - NOTNLM OT - Essential medicines OT - Perceived corruption vulnerability OT - Pharmaceutical procurement OT - Procurement stages OT - Public tertiary hospitals COIS- Declarations. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:28 PHST- 2026/03/27 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:28 [entrez] AID - 10.1186/s13561-026-00837-6 [pii] AID - 10.1186/s13561-026-00837-6 [doi] PST - epublish SO - Health Econ Rev. 2026 Aug 1;16(1):128. doi: 10.1186/s13561-026-00837-6. PMID- 42955508 OWN - NLM STAT- Publisher LR - 20261029 IS - 1568-5608 (Electronic) IS - 0925-4692 (Linking) DP - 2026 Oct 28 TI - Unraveling the role of CARM1 in Alzheimer's disease: molecular mechanisms and therapeutic potential. LID - 10.1007/s10787-026-02435-8 [doi] AB - Alzheimer's disease (AD) is a progressive neurological condition that has manifestations of cognitive decline, accumulation of neurofibrillary tau pathology, amyloid-beta (Abeta), synaptic dysfunction, neuroinflammation, oxidative stress, and cerebrovascular abnormalities. Evidence is growing that epigenetic dysfunction is involved in the development of AD. The coactivator-associated arginine methyltransferase 1 (CARM1), also known as protein arginine methyltransferase 4 (PRMT4), is an epigenetic enzyme that functions in transcriptional control, chromatin remodeling, maintenance of cellular homeostasis, and neuronal functions. CARM1-mediated histone H3 arginine 17 methylation (H3R17me2a) and NF-kappaB-dependent transcription provide a link to inflammatory gene regulation; however, a direct role for CARM1 in AD-associated neuroinflammation remains unconfirmed. Findings from cultured hippocampal neurons implicate CARM1 in dendritic maturation and synaptic organization, although these effects have not established therapeutic benefits in AD models. Evidence from female 3xTg-AD mice indicates that pharmacological CARM1 inhibition can improve selected cerebrovascular parameters, including nitric oxide synthase coupling and cerebral blood flow. CARM1 phosphorylation may also influence its enzymatic activity and localization, but its involvement in AD-related neuronal pathology remains hypothetical. The aim of this study is to put together in a comprehensive manner the role of CARM1 in the molecular and cellular mechanisms involved in AD pathology and its potential as a therapeutic target. Future studies should focus on brain penetrants and selective CARM1 modulators, cell-specific functions, long-term safety, target engagement, and clinical translation. In conclusion, CARM1 may be a potential AD therapeutic target that is still in the preclinical phase. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature Switzerland AG. FAU - Gupta, Sunit Kumar AU - Gupta SK AD - Department of Pharmacology, ISF College of Pharmacy, Moga, Punjab, 142001, India. FAU - Aran, Khadga Raj AU - Aran KR AUID- ORCID: 0000-0002-2450-2975 AD - Department of Pharmacology, ISF College of Pharmacy, Moga, Punjab, 142001, India. bishalarann@gmail.com. AD - Department of Pharmacy Practice, ISF College of Pharmacy, Moga, Punjab, 142001, India. bishalarann@gmail.com. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - Switzerland TA - Inflammopharmacology JT - Inflammopharmacology JID - 9112626 SB - IM OTO - NOTNLM OT - Alzheimer's disease OT - CARM1 OT - Cerebrovascular dysfunction OT - Epigenetic regulation OT - Neuroinflammation OT - PRMT4 OT - Synaptic plasticity COIS- Declarations. Conflict of interest: The authors declare no competing interests. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:28 PHST- 2026/09/19 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:28 [entrez] AID - 10.1007/s10787-026-02435-8 [pii] AID - 10.1007/s10787-026-02435-8 [doi] PST - aheadofprint SO - Inflammopharmacology. 2026 Oct 28. doi: 10.1007/s10787-026-02435-8. PMID- 42955507 OWN - NLM STAT- Publisher LR - 20261029 IS - 1573-2789 (Electronic) IS - 0010-3853 (Linking) DP - 2026 Oct 29 TI - Readmission and Treatment Adherence During and After Court-Ordered Treatment in Arizona: A Retrospective Cohort Study. LID - 10.1007/s10597-026-01741-3 [doi] AB - Court-Ordered Treatment (COT) is used to address treatment non-adherence and psychiatric readmission in individuals with serious mental illness (SMI), who are at risk for treatment non-adherence and recurrent psychiatric hospitalization. Evidence regarding clinical outcomes associated with COT remains mixed, partly due to differences in legal frameworks and program implementation. To compare psychiatric readmission, outpatient appointment attendance, and long-acting injectable (LAI) medication adherence during COT and during the year following COT expiration in Arizona's public behavioral health system. This exploratory, retrospective chart review identified 94 patients placed on COT between 2020 and 2021. Paired within-subject analyses included 29 patients whose COT expired and who completed a full one-year follow-up after COT expiration. Outcomes during COT were compared with outcomes during the year after COT expiration. Among the 29 patients analyzed, psychiatric hospital admission rates rose significantly from 10.3% (3/29) during COT to 34.5% (10/29) post COT expiration (p = .039). Missed psychiatric appointments increased from 24.1% (7/29) during COT to 37.9% (11/29) after COT expiration, but this change was not statistically significant (p =.219). Among 19 patients with complete LAI follow-up data, 1 missed at least one scheduled LAI dose during COT compared with 4 after COT expiration (p =.250). In this exploratory cohort, psychiatric readmissions were less frequent during COT than during the year following COT expiration. Both missed outpatient appointments and LAI doses were also more frequent after COT expiration, although these differences were not statistically significant, and the small sample limited the ability to detect meaningful differences. These findings should be interpreted cautiously given the small, selected cohort and retrospective observational design. Larger prospective studies are needed to further evaluate outcomes following COT expiration. CI - (c) 2026. The Author(s). FAU - Sood, Shabnam AU - Sood S AD - Department of Psychitary, Valleywise Health, Phoenix, United States. shabnam_sood@dmgaz.org. AD - Psychitary, Creighton university school of medicine, Phoenix, United States. shabnam_sood@dmgaz.org. FAU - Mannekote, Srinagesh AU - Mannekote S AD - Department of Psychitary, Valleywise Health, Phoenix, United States. AD - Psychitary, Creighton university school of medicine, Phoenix, United States. FAU - Filippelli, Jennifer AU - Filippelli J AD - Department of Psychitary, Valleywise Health, Phoenix, United States. AD - Psychitary, Creighton university school of medicine, Phoenix, United States. FAU - Dudeja, Neil AU - Dudeja N AD - Department of Psychitary, Valleywise Health, Phoenix, United States. AD - Psychitary, Creighton university school of medicine, Phoenix, United States. FAU - Ayoub, Alexander AU - Ayoub A AD - Department of Psychitary, Valleywise Health, Phoenix, United States. AD - Psychitary, Creighton university school of medicine, Phoenix, United States. FAU - Ramos, Gilbert AU - Ramos G AD - Department of Psychitary, Valleywise Health, Phoenix, United States. FAU - Mathieson, Kathleen AU - Mathieson K AD - Department of Psychitary, Valleywise Health, Phoenix, United States. AD - A.T. Still University, Kirksville, United States. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Community Ment Health J JT - Community mental health journal JID - 0005735 SB - IM OTO - NOTNLM OT - Assisted outpatient treatment OT - Community treatment orders OT - Court-ordered treatment OT - Long-acting injectable OT - Psychiatric admission OT - Serious mental illness OT - Treatment adherence COIS- Declarations. Competing Interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:28 PHST- 2026/04/15 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:28 [entrez] AID - 10.1007/s10597-026-01741-3 [pii] AID - 10.1007/s10597-026-01741-3 [doi] PST - aheadofprint SO - Community Ment Health J. 2026 Oct 29. doi: 10.1007/s10597-026-01741-3. PMID- 42955503 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2197-425X (Print) IS - 2197-425X (Linking) VI - 14 IP - 1 DP - 2026 Oct 28 TI - Adjunctive therapeutic plasma exchange as rescue therapy in septic shock due to streptococcal infection: a multicentric retrospective cohort study. LID - 142 [pii] LID - 10.1186/s40635-026-00984-4 [doi] AB - BACKGROUND: Septic shock due to streptococcal infection (SRSS) carries a high mortality and is in part driven by toxin-mediated inflammation. Therapeutic plasma exchange (TPE) may modulate the septic host responses by removing injurious mediators while replenishing protective plasma factors, but data specific to SRSS is lacking. We performed a retrospective, multicenter, cohort study of adults with SRSS between 2012-2024. METHODS: Patients from three tertiary care ECMO centers in Germany and Switzerland were included requiring (i) streptococcal spp. detected in blood or primary focus and (ii) septic shock according to sepsis-3 definition. Adjunctive TPE with fresh frozen plasma at the intensivists; discretion was compared with standard of care (SOC). The primary outcome was 90-day-hospital mortality. Key exploratory outcomes included 24-h kinetics of vasoactive-inotropic score(VIS) and lactate, as well as SRSS-related complications. Results were validated in a 1:1 propensity score-matched (PSM) cohort. RESULTS: Of 149 patients, 31 (20.8%) received TPE and 118 (79.2%) SOC. TPE patients were more severely ill at baseline: SOFA 14 (IQR 13.5-15) vs 11 (IQR 8-14) (p<0.001); lactate 6.4 vs 2.8 mmol/L (p<0.001); Vasoactive-Inotropic-Score (VIS) 70.7 vs 23.3 (p<0.001); more frequent invasive ventilation (87% vs 65%), VV-ECMO (36% vs 19%), and renal replacement therapy (96.8% vs 52.5%). Overall, 90-day hospital mortality was 27.5% (TPE 29.0%, SOC 27.1%). Following SOFA-score adjustment, TPE was associated with lower mortality risk (aHR 0.67 [95% CI 0.5-0.9]; p=0.009). This was validated in the PSM cohort, with an aHR for TPE of 0.44 (95% CI 0.3-0.8); p=0.002. Mixed-effects modeling demonstrated a significant VIS reduction only with TPE (fixed effect 24-h change, p=0.027) and improved lactate decrease versus SOC. CONCLUSION: In SRSS, adjunctive TPE was associated with rapid hemodynamic stabilization and improved lactate kinetics. Exploratory adjusted survival analyses support prospective evaluation of TPE in SRSS. CI - (c) 2026. The Author(s). FAU - Ruwisch, Jannik AU - Ruwisch J AUID- ORCID: 0000-0003-1654-6668 AD - Department of Respiratory Medicine and Infectious Diseases, Hannover Medical School, Hannover, Germany. AD - Biomedical Research in End-Stage and Obstructive Lung Disease (BREATH), Hannover Medical School (MHH), German Center for Lung Research (DZL), Hannover, Germany. AD - PRACTIS Clinician Scientist Program, Dean's Office for Academic Career Development, Hannover Medical School, Hannover, Germany. FAU - Buhlmann, Alix AU - Buhlmann A AD - Institute of Intensive Care Medicine, University and University Hospital Zurich, Zurich, Switzerland. FAU - Wild, Lennart AU - Wild L AD - Department of Anaesthesiology and Intensive Care Medicine, University Hospital Bonn, Bonn, Germany. FAU - Wendel-Garcia, Pedro David AU - Wendel-Garcia PD AD - Institute of Intensive Care Medicine, University and University Hospital Zurich, Zurich, Switzerland. AD - Division of Cardiothoracic Anaesthesia and Intensive Care Medicine, Department of General Anaesthesia, Intensive Care and Pain Medicine, Medical University Vienna, Vienna, Austria. FAU - Pape, Thorben AU - Pape T AD - Department of Respiratory Medicine and Infectious Diseases, Hannover Medical School, Hannover, Germany. AD - Biomedical Research in End-Stage and Obstructive Lung Disease (BREATH), Hannover Medical School (MHH), German Center for Lung Research (DZL), Hannover, Germany. AD - PRACTIS Clinician Scientist Program, Dean's Office for Academic Career Development, Hannover Medical School, Hannover, Germany. FAU - Hofmaenner, Daniel A AU - Hofmaenner DA AD - Institute of Intensive Care Medicine, University and University Hospital Zurich, Zurich, Switzerland. FAU - Putensen, Christian AU - Putensen C AD - Department of Anaesthesiology and Intensive Care Medicine, University Hospital Bonn, Bonn, Germany. FAU - Kruse, Philippe AU - Kruse P AD - Department of Anaesthesiology and Intensive Care Medicine, University Hospital Bonn, Bonn, Germany. FAU - Holtgrave, Greta-Henrike AU - Holtgrave GH AD - Department of Respiratory Medicine and Infectious Diseases, Hannover Medical School, Hannover, Germany. FAU - Schenk, Heiko AU - Schenk H AD - Department of Nephrology and Hypertension, Hannover Medical School, Hannover, Germany. FAU - Bode, Christian AU - Bode C AD - Department of Anaesthesiology and Intensive Care Medicine, University Hospital Bonn, Bonn, Germany. FAU - David, Sascha AU - David S AD - Institute of Intensive Care Medicine, University and University Hospital Zurich, Zurich, Switzerland. FAU - Stahl, Klaus AU - Stahl K AD - Department of Gastroenterology, Hepatology, Infectious Diseases and Endocrinology, Hannover Medical School, Hannover, Germany. FAU - Seeliger, Benjamin AU - Seeliger B AUID- ORCID: 0000-0001-7373-752X AD - Department of Respiratory Medicine and Infectious Diseases, Hannover Medical School, Hannover, Germany. seeliger.benjamin@mh-hannover.de. AD - Biomedical Research in End-Stage and Obstructive Lung Disease (BREATH), Hannover Medical School (MHH), German Center for Lung Research (DZL), Hannover, Germany. seeliger.benjamin@mh-hannover.de. CN - Bonn-Hannover-Zurich ARDS (BonHanZA) Study Group LA - eng GR - ME 3696/3-1/Deutsche Forschungsgemeinschaft/ PT - Journal Article DEP - 20261028 PL - Germany TA - Intensive Care Med Exp JT - Intensive care medicine experimental JID - 101645149 OTO - NOTNLM OT - Mortality OT - Sepsis OT - Septic shock OT - Streptococcus OT - Therapeutic plasma exchange COIS- Declarations. Ethics approval and consent to participate: All procedures performed in this study were in accordance with the Declaration of Helsinki, and the study was reviewed and approved by the respective institutional review boards of all three participating centers: 11221_BO_K_2024 (Hannover); BASEC 2024-00993 (Zurich); 437/23-EP (BONN). Consent was waived due to the retrospective nature of the study. Consent for publication: Not applicable. Competing interests: JR: Received lecturing fees from Boehringer Ingelheim and Astra Zeneca, all not related to this work.CB: functions as principal investigator for the EXCHANGE-2 Study, received lecturing fees Octapharma and CSL Behring, advisory board Artcline,SD: functions as principal investigator for the EXCHANGE-2 Study, received lecturing fees and travel compensations from Octapharma, Vantive and CSL Behring with regard to plasma exchange in sepsis. All other expert compensations, research support and advisory board activities are not related to the topic.KS: functions as principal investigator for the EXCHANGE-2 StudyBS: Received lecturing and consulting fees from Boehringer Ingelheim, Glaxo-Smith-Klein, AstraZeneca and Chiesi, all not related to this workAB, LW, TP, DAH, CP, PK, PDGW, GHH, HS: declare no competing interests. FIR - Hoeper, Marius M IR - Hoeper MM FIR - Peukert, Konrad IR - Peukert K FIR - Feuerborn, Caroline IR - Feuerborn C FIR - Schirmer, Finn IR - Schirmer F FIR - Sauer, Andrea IR - Sauer A FIR - Mueller, Mattia IR - Mueller M FIR - Schuepbach, Reto IR - Schuepbach R FIR - Kleinert, Eva-Maria IR - Kleinert EM FIR - Erlebach, Rolf IR - Erlebach R FIR - Andermatt, Rea IR - Andermatt R EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:28 PHST- 2026/07/02 00:00 [received] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:28 [entrez] AID - 10.1186/s40635-026-00984-4 [pii] AID - 10.1186/s40635-026-00984-4 [doi] PST - epublish SO - Intensive Care Med Exp. 2026 Oct 28;14(1):142. doi: 10.1186/s40635-026-00984-4. PMID- 42955502 OWN - NLM STAT- Publisher LR - 20261029 IS - 1573-4994 (Electronic) IS - 1053-0509 (Linking) DP - 2026 Oct 28 TI - Boron Doped Carbon Dot Integrated Fluorescent Hybrid Microgels: A Smart and Responsive Framework for Sensing of Salicylic Acid. LID - 10.1007/s10895-026-04980-5 [doi] AB - The increasing prevalence of salicylic acid as an unauthorized adulterant in milk and its derived products poses serious concerns for human health and safety. In this study, a fluorescent probe based on boron-doped carbon dot-integrated poly(N-isopropylacrylamide) microgels (BCQDs@PNIPAM) was developed for the selective and sensitive detection of salicylic acid. The incorporation of carbon quantum dots (CQDs) within the PNIPAM microgel matrix not only imparts strong fluorescence but also minimizes their inherent tendency to self-aggregate, thereby enabling a stimuli-responsive sensing platform for salicylic acid detection. The biocompatibility of the developed probe was further evaluated through seed germination and bacterial assays. Moreover, the introduction of electron-deficient boron into the CQDs enhances their fluorescence performance, improving selectivity and quick response with "turn-on" signal for salicylic acid. Using this strategy, the probe demonstrated a linear detection range of 1-240 microM with a low limit of detection (LOD) of 54 nM. The feasibility of the sensing platform was demonstrated in real samples, highlighting its potential in quality control in food products. In addition, the enhanced fluorescence characteristics of the hybrid microgels enabled their application in security and anti-counterfeiting systems. Overall, this work offers a reliable and sustainable sensing platform for ensuring milk quality and safety, with promising implications for food surveillance and public health protection. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Garg, Neha AU - Garg N AD - Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh, 160014, India. FAU - Muskan AU - Muskan AD - Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh, 160014, India. FAU - Dan, Abhijit AU - Dan A AD - Department of Applied Chemistry, Maulana Abul Kalam Azad University of Technology, Simhat, Haringhata, 741249, West Bengal, India. FAU - Chaudhary, Savita AU - Chaudhary S AD - Department of Chemistry and Centre of Advanced Studies in Chemistry, Panjab University, Chandigarh, 160014, India. schaudhary@pu.ac.in. LA - eng GR - IFACH-17/DST/ PT - Journal Article DEP - 20261028 PL - Netherlands TA - J Fluoresc JT - Journal of fluorescence JID - 9201341 SB - IM OTO - NOTNLM OT - Carbon dots OT - Fluorescence OT - Microgel OT - Salicylic acid COIS- Declarations. Ethical Approval and Clinical Trial Number.: Not Applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:28 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:28 [entrez] AID - 10.1007/s10895-026-04980-5 [pii] AID - 10.1007/s10895-026-04980-5 [doi] PST - aheadofprint SO - J Fluoresc. 2026 Oct 28. doi: 10.1007/s10895-026-04980-5. PMID- 42955501 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1863-2491 (Electronic) IS - 1863-2483 (Linking) VI - 20 IP - 1 DP - 2026 Oct 29 TI - Robot-assisted gastrectomy for locally advanced gastric cancer and complex clinical scenarios: technical evolution, evidence gaps, and future directions. LID - 1153 [pii] LID - 10.1007/s11701-026-04128-4 [doi] AB - Robot-assisted gastrectomy has evolved over two decades from a technical curiosity into a feasible operative option, but its role in locally advanced gastric cancer and complex clinical scenarios remains unsettled. This narrative review is based on a documented search of PubMed/MEDLINE, Embase, Scopus, Web of Science, the Cochrane Library, and the China National Knowledge Infrastructure (January 2002 through September 2026) and synthesizes evidence on technical evolution, perioperative and oncological outcomes, and the use of robotic platforms in obesity, advanced age, high comorbidity burden, post-neoadjuvant fibrosis, and total gastrectomy with esophagojejunostomy. Most comparative evidence is observational (propensity-matched and single-center), and evidence for advanced or complex disease is partly extrapolated from early-stage and mixed-stage cohorts. Robotic gastrectomy is associated consistently with less blood loss and, in some series, higher lymph node yields at anatomically demanding stations, whereas operative time is longer, overall complication rates are generally similar, and no adequately powered randomized trial has shown a survival advantage. One randomized phase II trial in distal gastrectomy met a non-inferiority criterion for 3-year disease-free survival; an exploratory survival difference in that trial and observational signals of improved recurrence-free survival in stage III disease remain hypothesis-generating. High-quality patient-reported and functional outcomes, and data from non-East Asian populations, are scarce. Higher costs, a learning curve of approximately 25-50 cases, and the absence of phase III superiority data temper broad endorsement. Single-port systems, alternative platforms, and artificial-intelligence and augmented-reality tools are emerging but are not yet validated in gastric cancer surgery. We map each claim to its source population, distinguish direct from extrapolated evidence, and identify the trial designs and patient-centered endpoints needed to define the appropriate role of robotic gastrectomy. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature. FAU - Zhu, Xiangyu AU - Zhu X AD - Department of General Medicine, Cancer Hospital of Dalian University of Technology, Liaoning Cancer Hospital & Institute, Shenyang, China. FAU - Gao, Jun AU - Gao J AD - Department of Thyroid, Breast, Hernia, Vascular, and Bariatric Surgery, Dandong Central Hospital, Dandong, China, Liaoning. FAU - Wang, Tianzuo AU - Wang T AD - International Department, Shenyang Feiyue Experimental School, Shenyang, China. AD - Avaline School, Shenyang, China. FAU - Wang, Tianyou AU - Wang T AD - International Department, Shenyang Feiyue Experimental School, Shenyang, China. AD - Avaline School, Shenyang, China. FAU - Wang, Yang AU - Wang Y AD - Department of rehabilitation, The Second Affliated Hospital of Shenyang Medical College, Shenyang, China. FAU - Wang, Yue AU - Wang Y AD - Department of International Medical Services (Surgical Ward), Cancer Hospital of Dalian University of Technology, Liaoning Cancer Hospital & Institute, Shenyang, China. 18900917529@163.com. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - J Robot Surg JT - Journal of robotic surgery JID - 101300401 SB - IM MH - Humans MH - *Stomach Neoplasms/surgery/pathology MH - *Gastrectomy/methods/trends MH - *Robotic Surgical Procedures/methods/trends MH - Evidence Gaps MH - Operative Time MH - Treatment Outcome MH - Neoplasm Staging OTO - NOTNLM OT - D2 lymphadenectomy OT - Gastric cancer OT - Locally advanced gastric cancer OT - Minimally invasive surgery OT - Narrative review OT - Quality of life OT - Robotic gastrectomy COIS- Declarations. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. Ethics approval: Not applicable. Consent to participate: Not applicable. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:27 PHST- 2026/10/14 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:27 [entrez] AID - 10.1007/s11701-026-04128-4 [pii] AID - 10.1007/s11701-026-04128-4 [doi] PST - epublish SO - J Robot Surg. 2026 Oct 29;20(1):1153. doi: 10.1007/s11701-026-04128-4. PMID- 42955499 OWN - NLM STAT- Publisher LR - 20261029 IS - 1573-1677 (Electronic) IS - 1382-4996 (Linking) DP - 2026 Oct 29 TI - Toward multimodal physiological assessment of clinical performance in simulation-based medical education: a machine learning approach. LID - 10.1007/s10459-026-10590-4 [doi] AB - BACKGROUND: Assessment of learner performance in simulation-based medical education commonly relies on faculty observation. Learners' physiological responses may provide complementary information about responses to clinical task demands, but their relationship with observed performance is complex and context dependent. Whether multimodal physiological patterns can reliably distinguish faculty-assigned performance categories across simulation events and learner populations remains unclear. METHODS: This prospective observational study developed machine-learning models using physiological data alone to classify learner performance according to faculty-assigned novice versus competent categories during high-fidelity simulation. Data were collected from 152 learners (74 Emergency Medicine residents, 70 Anesthesiology residents, 8 Emergency Medical Services students) across 470 simulation scenarios. A multimodal assessment platform synchronized physiological signals (electrodermal activity, heart rate, skin temperature) from Empatica E4 wristbands with faculty evaluations. A genetic algorithm was employed for feature selection, and neural network models were evaluated using multiple leave-N-out strategies to assess generalizability across learners. RESULTS: The neural network achieved 84-85% balanced accuracy across thresholds 0.45-0.70, with sensitivity 93.3-95.4% and specificity 72.9-76.2%. Despite class imbalance (80.6% competent, 19.4% novice), performance remained robust, with Matthews correlation coefficients of 0.687-0.706 and precision-recall area-under-the-curve (PR-AUC) values of 0.969-0.970 across thresholds. CONCLUSIONS: Multimodal physiological features enabled classification of faculty-assigned performance categories with strong discrimination in internal validation. These findings support the potential of temporally resolved, physiology-informed analytics to complement faculty assessment and facilitate targeted review of simulation events. Further work is needed to strengthen rating-label validity and temporal alignment and to evaluate model transparency, fairness, and generalizability across institutions, simulation contexts, and learner populations. CI - (c) 2026. The Author(s). FAU - Teye-Lartey, Solomon Prince AU - Teye-Lartey SP AUID- ORCID: 0009-0005-7719-6341 AD - Department of Bioengineering , College of Engineering, University of Toledo, Toledo, OH, USA. FAU - Schmieder, Jacob AU - Schmieder J AD - College of Medicine and Life Sciences, University of Toledo, Toledo, OH, USA. FAU - Yadav, Umesh AU - Yadav U AD - Department of Electrical Engineering and Computer Science, College of Engineering, University of Toledo, Toledo, OH, USA. FAU - Aouthmany, Shaza AU - Aouthmany S AD - Department of Emergency Medicine, College of Medicine and Life Sciences, University of Toledo, Toledo, OH, USA. FAU - Alvarado, Cristina AU - Alvarado C AD - College of Medicine and Life Sciences, Interprofessional Immersive Simulation Center, University of Toledo, Toledo, OH, USA. FAU - Jenkins, Kimberly AU - Jenkins K AD - Department of Anesthesiology, College of Medicine and Life Sciences, University of Toledo, Toledo, OH, 43614, USA. FAU - Papadimos, Thomas J AU - Papadimos TJ AD - Department of Anesthesiology, College of Medicine and Life Sciences, University of Toledo, Toledo, OH, 43614, USA. FAU - Toy, Serkan AU - Toy S AUID- ORCID: 0000-0001-7758-257X AD - Departments of Basic Science Education and Health Systems & Implementation Science, Virginia Tech Carilion School of Medicine, Roanoke, VA, USA. FAU - Brickman, Kris AU - Brickman K AD - Department of Bioengineering , College of Engineering, University of Toledo, Toledo, OH, USA. FAU - Braida, Anthony L AU - Braida AL AD - Department of Anesthesiology, College of Medicine and Life Sciences, University of Toledo, Toledo, OH, 43614, USA. FAU - Altenhof, Brent AU - Altenhof B AD - Department of Anesthesiology, College of Medicine and Life Sciences, University of Toledo, Toledo, OH, 43614, USA. FAU - Pappada, Scott M AU - Pappada SM AUID- ORCID: 0000-0002-3877-6854 AD - Department of Bioengineering , College of Engineering, University of Toledo, Toledo, OH, USA. scott.pappada@utoledo.edu. AD - Department of Electrical Engineering and Computer Science, College of Engineering, University of Toledo, Toledo, OH, USA. scott.pappada@utoledo.edu. AD - Department of Anesthesiology, College of Medicine and Life Sciences, University of Toledo, Toledo, OH, 43614, USA. scott.pappada@utoledo.edu. LA - eng GR - HT942524C0073/Congressionally Directed Medical Research Programs/ GR - HT942524C0073/Congressionally Directed Medical Research Programs/ GR - HT942524C0073/Congressionally Directed Medical Research Programs/ PT - Journal Article DEP - 20261029 PL - Netherlands TA - Adv Health Sci Educ Theory Pract JT - Advances in health sciences education : theory and practice JID - 9612021 SB - IM OTO - NOTNLM OT - Competency assessment OT - Machine learning OT - Multimodal assessment OT - Physiological monitoring OT - Simulation-based medical education OT - Wearable sensors COIS- Declarations. Ethics approval: This study received Institutional Review Board approval from the University of Toledo. All procedures involving human participants were performed in accordance with the ethical standards of the institutional research committee and with the 1964 Declaration of Helsinki and its later amendments. Written informed consent was obtained from all participants. Participants were informed that performance data would be used exclusively for research purposes and would not impact their academic standing. Consent to participate: Written informed consent was obtained from all individual participants included in the study. Consent for publication: Not applicable. This manuscript does not contain identifiable individual participant data. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:27 PHST- 2026/02/10 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:27 [entrez] AID - 10.1007/s10459-026-10590-4 [pii] AID - 10.1007/s10459-026-10590-4 [doi] PST - aheadofprint SO - Adv Health Sci Educ Theory Pract. 2026 Oct 29. doi: 10.1007/s10459-026-10590-4. PMID- 42955498 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1534-6277 (Electronic) IS - 1534-6277 (Linking) VI - 27 IP - 1 DP - 2026 Oct 28 TI - Image-Guided Ablative Options for Desmoid Fibromatosis: Contemporary Evidence and Guideline Integration. LID - 51 [pii] LID - 10.1007/s11864-026-01422-z [doi] AB - Most patients with a newly diagnosed desmoid tumor should begin with multidisciplinary evaluation and active surveillance rather than immediate intervention, provided that symptoms are limited and further growth is unlikely to create irreversible morbidity. Treatment should be initiated when pain, functional limitation, sustained progression, or threatened critical structures make continued observation unacceptable. Percutaneous cryoablation is generally favored because the ice ball can be monitored directly, treatment can be personalized/tailored with the synergistic benefit of multiple probes, and the procedure can be repeated or staged when a complete margin would be unsafe. Complete ablation should be targeted when the entire tumor can be covered without jeopardizing skin, nerves, bowel, or other vulnerable structures; deliberate subtotal ablation is appropriate when the principal goal is pain relief or functional improvement and a safe circumferential margin is not achievable. Hydrodissection, skin warming, neural protection, and conservative treatment near critical anatomy are essential. Magnetic resonance-guided focused ultrasound is an attractive incisionless alternative for tumors with a favorable acoustic window. Microwave or radiofrequency ablation should be reserved for carefully selected lesions and experienced operators; electrochemotherapy and chemical ablation remain investigational with some promising early evidence. Embolization can be useful for hypervascular tumors that are poorly suited to direct percutaneous treatment. Local and systemic therapy should not be viewed as mutually exclusive: systemic treatment is often preferable for diffuse, intra-abdominal, or anatomically hazardous disease, while ablation can address a dominant symptomatic focus, residual viable tumor, or focal progression. Further study into the benefits and sequencing of combining local therapy with systemic or targeted options will be helpful in the multi-disciplinary evaluation of this complex patient cohort. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Adamo, Daniel A AU - Adamo DA AD - Department of Radiology, Mayo Clinic, Rochester, MN, USA. FAU - Schmitz, John J AU - Schmitz JJ AD - Department of Radiology, Mayo Clinic, Rochester, MN, USA. FAU - Welch, Tasha L AU - Welch TL AD - Department of Anesthesiology, Mayo Clinic, Rochester, MN, USA. FAU - Atwell, Thomas D AU - Atwell TD AD - Department of Radiology, Mayo Clinic, Rochester, MN, USA. FAU - Siontis, Brittany L AU - Siontis BL AD - Department of Medical Oncology, Mayo Clinic, Rochester, MN, USA. FAU - Welch, Brian T AU - Welch BT AD - Department of Radiology, Mayo Clinic, Rochester, MN, USA. welch.brian@mayo.edu. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Curr Treat Options Oncol JT - Current treatment options in oncology JID - 100900946 SB - IM MH - Humans MH - *Desmoid Tumors/surgery/therapy/diagnostic imaging/diagnosis MH - *Surgery, Computer-Assisted/methods MH - Cryosurgery/methods MH - Treatment Outcome MH - Practice Guidelines as Topic COIS- Declarations. Ethics Approval and Consent to Participate: Not applicable. Competing interests: The authors declare no competing interests. Use of Generative Artificial Intelligence: A generative artificial intelligence tool was used to assist with initial drafting and organization. The authors critically reviewed and revised all content. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:27 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:27 [entrez] AID - 10.1007/s11864-026-01422-z [pii] AID - 10.1007/s11864-026-01422-z [doi] PST - epublish SO - Curr Treat Options Oncol. 2026 Oct 28;27(1):51. doi: 10.1007/s11864-026-01422-z. PMID- 42955495 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1534-6277 (Electronic) IS - 1534-6277 (Linking) VI - 27 IP - 1 DP - 2026 Oct 28 TI - Treatment Options After Recurrence Following Perioperative Durvalumab Plus FLOT for Gastric and Gastroesophageal Junction Adenocarcinoma: A Biology-First Sequencing Framework. LID - 50 [pii] LID - 10.1007/s11864-026-01416-x [doi] AB - Perioperative durvalumab plus fluorouracil, leucovorin, oxaliplatin, and docetaxel (FLOT) has established a new curative-intent standard for resectable gastric and gastroesophageal junction adenocarcinoma. Recurrence after this regimen creates a clinically distinct, pre-exposed metastatic state for which no prospective sequencing standard exists. Because direct post-D-FLOT evidence is absent, treatment decisions should distinguish exposure-analogous evidence from extrapolation from conventional metastatic trials. In practice, we first determine whether recurrence after perioperative durvalumab plus fluorouracil, leucovorin, oxaliplatin, and docetaxel (FLOT) is potentially amenable to local treatment and, if systemic therapy is required, document the recurrence phase, the dates of the last FLOT and durvalumab doses, prior radiographic and pathological response, residual neuropathy, immune-related adverse events, organ function, performance status, and disease tempo. We then obtain recurrent-tumor tissue when feasible and reassess human epidermal growth factor receptor 2 (HER2), claudin 18.2 (CLDN18.2), and microsatellite instability/mismatch repair status; programmed death-ligand 1 results are interpreted in the context of assay differences and prior durvalumab exposure. If HER2 is positive and no prior HER2-directed therapy was given, we prioritize a HER2-directed strategy. If CLDN18.2 is positive and HER2 negative, zolbetuximab-based therapy is reasonable when oxaliplatin re-use is clinically defensible, but very early relapse or persistent neuropathy should favor an alternative strategy or a clinical trial. Microsatellite instability-high or mismatch repair-deficient disease remains immunologically compelling, although checkpoint-inhibitor re-use after durvalumab is not established. In biomarker-negative disease recurring during treatment or within about 6 months, we favor a non-cross-resistant approach, using ramucirumab- or irinotecan-based therapy according to prior taxane exposure, neuropathy, and regional availability. With a longer treatment-free interval and recovered toxicity, selective fluoropyrimidine/platinum reintroduction becomes more reasonable. We avoid routine checkpoint-inhibitor rechallenge solely because programmed death-ligand 1 is positive. For trial eligibility and line-of-therapy nomenclature, we count recurrence within 12 months of the last administered perioperative systemic dose (chemotherapy or durvalumab, whichever was later) as one prior systemic line, while recognizing that this is a pragmatic classification rather than a validated biologic cutoff. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Sagawa, Tamotsu AU - Sagawa T AUID- ORCID: 0000-0002-1994-4795 AD - Department of Gastroenterology, National Hospital Organization Hokkaido Cancer Center, 2-3-54 Kikusui 4-jo, Shiroishi-ku, Sapporo, Hokkaido, 003-0804, Japan. sagawa.tamotsu.dy@mail.hosp.go.jp. FAU - Hirakawa, Masahiro AU - Hirakawa M AD - Division of Medical Oncology, Department of Internal Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan. FAU - Nagashima, Hiroyuki AU - Nagashima H AD - Department of Gastroenterology, National Hospital Organization Hokkaido Cancer Center, 2-3-54 Kikusui 4-jo, Shiroishi-ku, Sapporo, Hokkaido, 003-0804, Japan. FAU - Fujikawa, Koshi AU - Fujikawa K AD - Department of Gastroenterology, National Hospital Organization Hokkaido Cancer Center, 2-3-54 Kikusui 4-jo, Shiroishi-ku, Sapporo, Hokkaido, 003-0804, Japan. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Curr Treat Options Oncol JT - Current treatment options in oncology JID - 100900946 RN - 28X28X9OKV (durvalumab) RN - 0 (Antibodies, Monoclonal) RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - *Adenocarcinoma/drug therapy/pathology/etiology/therapy/diagnosis/mortality MH - *Esophagogastric Junction/pathology MH - *Stomach Neoplasms/drug therapy/pathology/therapy/etiology MH - Antibodies, Monoclonal/administration & dosage/therapeutic use MH - *Antineoplastic Combined Chemotherapy Protocols/therapeutic use/adverse effects MH - *Neoplasm Recurrence, Local MH - *Esophageal Neoplasms/drug therapy/pathology/etiology/therapy MH - Treatment Outcome MH - Biomarkers, Tumor OTO - NOTNLM OT - Durvalumab OT - FLOT OT - Gastric cancer OT - Gastroesophageal junction adenocarcinoma OT - Recurrence OT - Treatment sequencing COIS- Declarations. Ethics approval: Not applicable. This review did not involve new research with human participants or animals and does not contain clinical studies or patient data. Consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: Tamotsu Sagawa has received lecture honoraria from Ono Pharmaceutical Co., Ltd., Bristol Myers Squibb, Astellas Pharma Inc., Eli Lilly Japan K.K., and MSD K.K., and received advisory/consulting fees from Takeda Pharmaceutical Company Limited for a short-term agreement from July to August 2026. Masahiro Hirakawa, Hiroyuki Nagashima, and Koshi Fujikawa declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:27 PHST- 2026/08/22 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:27 [entrez] AID - 10.1007/s11864-026-01416-x [pii] AID - 10.1007/s11864-026-01416-x [doi] PST - epublish SO - Curr Treat Options Oncol. 2026 Oct 28;27(1):50. doi: 10.1007/s11864-026-01416-x. PMID- 42955493 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1534-312X (Electronic) IS - 1522-8037 (Linking) VI - 28 IP - 1 DP - 2026 Oct 29 TI - The Importance of Early (Effective) Interventions in Crohn's Disease: Current Concepts and Emerging Evidence. LID - 37 [pii] LID - 10.1007/s11894-026-01062-1 [doi] AB - PURPOSE OF REVIEW: Crohn's disease typically presents with primarily, luminal disease that progresses transmurally, producing structural bowel damage (strictures and fistula). This review examines the rationale for early treatment in Crohn's disease, summarizing the clinical evidence supporting early intervention to prevent transmural progression, an increased inflammatory burden and associated refractory disease. RECENT FINDINGS: Translational studies show that transmural inflammation shifts from a reversible to an irreversible, fibrotic process over time. Randomized trials, including the PROFILE study, demonstrate that early combined or top-down therapy improves long-term outcomes compared with conventional step-up management. Similar benefits have since been reported across non-anti-TNF therapeutic classes, suggesting that treatment responsiveness declines with disease duration as a shared biological feature of early disease, rather than a drug-specific effect. Emerging evidence also indicates that immune and mucosal changes may be detectable years before clinical recognition, raising the question of whether this decline in responsiveness begins even earlier than currently appreciated. Early treatment in Crohn's disease is associated with better long-term outcomes across multiple classes of advanced therapies. However, disease duration alone is an insufficient marker of where a patient stands in this process. Future efforts should refine the biological definition of early disease, improve patient selection, and move toward disease interception. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - da Silva, Bruno Cesar AU - da Silva BC AD - Division of Gastroenterology & Hepatology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada. bruno.cesardasilva@sinaihealth.ca. AD - Zane Cohen Centre for Digestive Diseases, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada. bruno.cesardasilva@sinaihealth.ca. AD - Division of Gastroenterology, Hospital da Bahia, Salvador, Bahia, Brazil. bruno.cesardasilva@sinaihealth.ca. FAU - de Azevedo, Matheus Freitas Cardoso AU - de Azevedo MFC AUID- ORCID: 0000-0001-5487-9418 AD - Department of Gastroenterology, University of Sao Paulo School of Medicine, Sao Paulo, SP, Brazil. FAU - Steinhart, A Hillary AU - Steinhart AH AD - Division of Gastroenterology & Hepatology, Temerty Faculty of Medicine, University of Toronto, Toronto, ON, Canada. AD - Zane Cohen Centre for Digestive Diseases, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada. FAU - Hanauer, Stephen B AU - Hanauer SB AD - Division of Gastroenterology, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - United States TA - Curr Gastroenterol Rep JT - Current gastroenterology reports JID - 100888896 SB - IM MH - Humans MH - *Crohn Disease/drug therapy/therapy/diagnosis MH - Disease Progression MH - *Early Medical Intervention/methods MH - Treatment Outcome OTO - NOTNLM OT - Advanced therapy OT - Crohn's disease OT - Disease interception OT - Disease modification OT - Early intervention OT - Structural damage COIS- Declarations. Competing interests: Bruno Cesar da Silva has served as a speaker for Johnson & Johnson, AbbVie, and Takeda. Matheus Freitas Cardoso de Azevedo has received fees for serving as a speaker and/or advisory board member for AbbVie, Janssen, and Takeda. A. Hillary Steinhart has served on advisory boards for AbbVie, Amgen, BioJAMP, BMS, Celltrion, Fresenius Kabi, Janssen, Lilly, Organon, OSE Immunotherapeutics, Pendopharm, Pfizer, and Sandoz; has served as a speaker for AbbVie, Janssen, Organon, Pfizer, and Takeda; has served as an investigator for AbbVie, Celgene/BMS, Genentech/Roche, Gilead, Janssen, Pfizer, Spyre Therapeutics, and Takeda; and has served as a consultant for Greenshield/NKS Pharmacy. Stephen B. Hanauer has received financial research, speaking, review, and/or consulting support from AbbVie, Allergan, Amgen, Arena, AstraZeneca, Boehringer Ingelheim, Bristol Myers Squibb, Celgene, Cosmos, Catalys Pacific, Covance, Genentech, GSK, Janssen, Lilly, Merck, Novartis, Pfizer, Progenity, Prometheus, Receptos, Salix, Samsung Bioepis, Seres Therapeutics, Sorriso, Takeda, TLL Pharma, UCB Pharma, VHsquared, Gossamer, and Protagonist. He also serves as Editor-in-Chief of Current Gastroenterology Reports. Declaration of AI-Assisted Tools: Grammarly add-in for Microsoft Word was used to check grammar and clarity during the preparation of this manuscript. The author(s) thoroughly reviewed and edited the content as needed, taking full responsibility for the final version of the publication. No AI tools were used for content generation. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:27 PHST- 2026/08/21 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:27 [entrez] AID - 10.1007/s11894-026-01062-1 [pii] AID - 10.1007/s11894-026-01062-1 [doi] PST - epublish SO - Curr Gastroenterol Rep. 2026 Oct 29;28(1):37. doi: 10.1007/s11894-026-01062-1. PMID- 42955488 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1863-2491 (Electronic) IS - 1863-2483 (Linking) VI - 20 IP - 1 DP - 2026 Oct 28 TI - Quantifying laparoscopic camera workspace and kinematics during robot-assisted radical prostatectomy. LID - 1152 [pii] LID - 10.1007/s11701-026-04132-8 [doi] AB - Robotic surgery has grown significantly in recent years because, in addition to high precision, the most technologically advanced systems provide surgeons with a high-quality three-dimensional (3D) view. Although studies on autonomous camera control are beginning to emerge, there are still limited data on laparoscopic camera motion, including its kinematics and workspace requirements during surgery. Therefore, this study aims to quantify the 3D workspace and motion metrics, including speed and absolute tangential acceleration, of the laparoscopic camera during robot-assisted radical prostatectomy (RARP). This single-centre observational study included ten RARP procedures performed by two surgeons using the da Vinci Xi system (Intuitive Surgical, CA, USA). Camera motion was recorded using a Polaris Vega optical tracking system (NDI, ON, Canada). A reference marker fixed to the robotic platform defined the local coordinate system, and a second marker was attached to the top of the camera. At the end of each surgery, a postoperative calibration was performed to determine the camera-tip position relative to the camera marker. Workspace and motion metrics were computed from valid samples across each recording and within individual surgical phases. Cleaning intervals and periods of marker occlusion or tracking interference were excluded from the analysis. Median valid tracking duration was 36.6 (32.1-63.3) min, with a median valid-sample coverage of 58.2 (38.6-67.9)%. The median complete-procedure workspace bounding-box volume was 356.56 (259.49-791.42) cm(3), whereas the median convex-hull volume was 104.35 (81.06-219.51) cm(3). The largest phase-specific workspace was observed during vesicourethral anastomosis, whereas the smallest was observed during endopelvic fascia incision. Motion metrics were comparatively consistent across surgical phases. This study quantified the laparoscopic camera workspace and characterized its motion during real RARP procedures, providing preliminary quantitative data to support the development of future autonomous robotic camera-control systems for RARP. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature. FAU - Pereira, Sergio G AU - Pereira SG AUID- ORCID: 0000-0002-2704-3831 AD - 2Ai - Applied Artificial Intelligence Laboratory, School of Technology, Polytechnic University of Cavado and Ave (UPCA), Barcelos, Portugal. sfpereira@ipca.pt. AD - Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Guimaraes, Portugal. sfpereira@ipca.pt. AD - LASI - Intelligent Systems Associate Laboratory, Guimaraes, Portugal. sfpereira@ipca.pt. FAU - Morais, Pedro AU - Morais P AD - 2Ai - Applied Artificial Intelligence Laboratory, School of Technology, Polytechnic University of Cavado and Ave (UPCA), Barcelos, Portugal. AD - LASI - Intelligent Systems Associate Laboratory, Guimaraes, Portugal. FAU - Queiros, Sandro AU - Queiros S AD - 2Ai - Applied Artificial Intelligence Laboratory, School of Technology, Polytechnic University of Cavado and Ave (UPCA), Barcelos, Portugal. AD - LASI - Intelligent Systems Associate Laboratory, Guimaraes, Portugal. FAU - Rocha, Fernando AU - Rocha F AD - 2Ai - Applied Artificial Intelligence Laboratory, School of Technology, Polytechnic University of Cavado and Ave (UPCA), Barcelos, Portugal. AD - LASI - Intelligent Systems Associate Laboratory, Guimaraes, Portugal. FAU - Rodrigues, Nuno AU - Rodrigues N AD - 2Ai - Applied Artificial Intelligence Laboratory, School of Technology, Polytechnic University of Cavado and Ave (UPCA), Barcelos, Portugal. AD - Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Braga, Guimaraes, Portugal. AD - LASI - Intelligent Systems Associate Laboratory, Guimaraes, Portugal. FAU - Quintelas, Ana AU - Quintelas A AD - Centro Hospitalar Universitario de Santo Antonio, Porto, Portugal. FAU - Lima, Estevao AU - Lima E AD - School of Medicine, University of Lisbon, Lisbon, Portugal. FAU - Vilaca, Joao L AU - Vilaca JL AD - 2Ai - Applied Artificial Intelligence Laboratory, School of Technology, Polytechnic University of Cavado and Ave (UPCA), Barcelos, Portugal. AD - LASI - Intelligent Systems Associate Laboratory, Guimaraes, Portugal. LA - eng PT - Journal Article PT - Observational Study DEP - 20261028 PL - England TA - J Robot Surg JT - Journal of robotic surgery JID - 101300401 SB - IM MH - *Prostatectomy/methods/instrumentation MH - Humans MH - *Robotic Surgical Procedures/methods/instrumentation MH - Male MH - *Laparoscopy/methods/instrumentation MH - Biomechanical Phenomena MH - Motion Capture OTO - NOTNLM OT - Laparoscopic camera motion OT - Laparoscopic camera workspace OT - Minimally invasive surgery OT - Robotic camera-holder systems OT - Robotic surgery OT - Surgical robots COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:27 PHST- 2026/09/24 00:00 [received] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:27 [entrez] AID - 10.1007/s11701-026-04132-8 [pii] AID - 10.1007/s11701-026-04132-8 [doi] PST - epublish SO - J Robot Surg. 2026 Oct 28;20(1):1152. doi: 10.1007/s11701-026-04132-8. PMID- 42955487 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2192-4449 (Electronic) IS - 2192-4449 (Linking) VI - 15 IP - 6 DP - 2026 Oct 28 TI - Renal vein thrombosis with inferior vena cava extension and pulmonary embolism in a patient with acute pyelonephritis: a case report and updated literature review. LID - 164 [pii] LID - 10.1007/s13730-026-01206-2 [doi] AB - Renal vein thrombosis (RVT) is a rare and underrecognized complication of acute pyelonephritis, with nephrotic syndrome and malignancy remaining the predominant causes of RVT. Because its clinical features overlap with those of the underlying infection, the diagnosis is often delayed until thrombus propagation or embolization occurs. A previously healthy 29-year-old man presented with a two-week history of left flank pain and fever. Contrast-enhanced computed tomography demonstrated acute left pyelonephritis with extensive renal vein thrombosis extending into the suprarenal inferior vena cava. Despite initiation of anticoagulation, pulmonary embolism developed during hospitalization. Cultures did not identify a causative organism; a low-count Staphylococcus haemolyticus isolate was considered a contaminant. Thrombophilia screening was negative. The patient improved with antimicrobial therapy and anticoagulation alone, requiring no invasive intervention. A PubMed search identified 23 previously published cases of acute pyelonephritis-associated RVT, pooled with the present case (n = 24) and analyzed separately from five xanthogranulomatous pyelonephritis-associated cases. Three cases were excluded for systemic or multifactorial thrombosis, and one case of RVT radiologically mimicking pyelonephritis. Gram-negative organisms predominated (78.3%), diabetes mellitus was present in fewer than half of patients, and outcomes were favorable with antimicrobial therapy and anticoagulation alone in most cases, while invasive interventions were reported selectively in cases with extensive thrombus burden or uncontrolled infection. RVT should be considered in patients with suspected pyelonephritis who deteriorate despite appropriate therapy or present with atypical clinical or imaging features. In cases where infection and RVT coexist, the temporal relationship and causality may remain uncertain, and diagnosis and management should be individualized according to the clinical presentation, thrombus extent, severity of infection, and imaging findings. CI - (c) 2026. The Author(s), under exclusive licence to Japanese Society of Nephrology. FAU - Sutanto, Ratna AU - Sutanto R AD - Department of Radiology of Siloam Hospital Lippo Village, Jl. MH Thamrin Boulevard 1100, Kecamatan Kelapa Dua, Kota Tangerang, Banten, Indonesia. ratnasutanto.raduph@gmail.com. AD - Pediatric Imaging Division, Radiology Department, Faculty of Medicine, Universitas Pelita Harapan, Tangerang, Banten, Indonesia. ratnasutanto.raduph@gmail.com. FAU - Koesbandono AU - Koesbandono AD - Department of Radiology of Siloam Hospital Lippo Village, Jl. MH Thamrin Boulevard 1100, Kecamatan Kelapa Dua, Kota Tangerang, Banten, Indonesia. FAU - Masui, Marshita AU - Masui M AD - Department of Radiology of Siloam Hospital Lippo Village, Jl. MH Thamrin Boulevard 1100, Kecamatan Kelapa Dua, Kota Tangerang, Banten, Indonesia. FAU - Octavius, Gilbert Sterling AU - Octavius GS AD - Department of Radiology of Siloam Hospital Lippo Village, Jl. MH Thamrin Boulevard 1100, Kecamatan Kelapa Dua, Kota Tangerang, Banten, Indonesia. LA - eng PT - Case Reports PT - Journal Article PT - Review DEP - 20261028 PL - Japan TA - CEN Case Rep JT - CEN case reports JID - 101636244 RN - 0 (Anticoagulants) RN - 0 (Anti-Bacterial Agents) SB - IM MH - Humans MH - Male MH - *Pyelonephritis/complications/drug therapy MH - Adult MH - *Pulmonary Embolism/drug therapy/etiology/diagnosis MH - *Venous Thrombosis/drug therapy/etiology/diagnosis/diagnostic imaging MH - *Vena Cava, Inferior/pathology/diagnostic imaging MH - *Renal Veins/pathology/diagnostic imaging MH - Tomography, X-Ray Computed/methods MH - Anticoagulants/therapeutic use MH - Acute Disease MH - Anti-Bacterial Agents/therapeutic use MH - Flank Pain/etiology MH - Treatment Outcome OTO - NOTNLM OT - Acute pyelonephritis OT - Inferior vena cava thrombosis OT - Pulmonary embolism OT - Renal vein thrombosis OT - Thrombo-inflammation COIS- Declarations. Conflict of interest: The authors declare that they have no competing interests. Ethical approval: Ethical approval is not needed for a case report in Indonesia. Written informed consent to participate was obtained from the patient before inclusion in this report. Consent for publication: Written informed consent for publication of clinical details and imaging was obtained from the patient. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:27 PHST- 2026/08/09 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:27 [entrez] AID - 10.1007/s13730-026-01206-2 [pii] AID - 10.1007/s13730-026-01206-2 [doi] PST - epublish SO - CEN Case Rep. 2026 Oct 28;15(6):164. doi: 10.1007/s13730-026-01206-2. PMID- 42955484 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1559-0100 (Electronic) IS - 1355-008X (Linking) VI - 91 IP - 1 DP - 2026 Oct 29 TI - Dual-Energy X-Ray absorptiometry based differentiation of lipoedema and familial partial lipodystrophy from general obesity in women. LID - 355 [pii] LID - 10.1007/s12020-026-04723-3 [doi] AB - BACKGROUND: Lipoedema and familial partial lipodystrophy (FPLD) are rare adipose tissue disorders frequently misclassified as common obesity. Clinical assessment and body mass index (BMI) alone may fail to capture regional fat distribution, delaying recognition and suboptimal management of metabolic complications. OBJECTIVE: To evaluate whether dual-energy X-ray absorptiometry (DXA)-derived regional fat distribution indices may provide complementary phenotyping information for distinguishing clinically characterized women with lipoedema, FPLD, general obesity, and normal adiposity. DXA-derived ratios and percentage fat mass were evaluated as exploratory, descriptive measures rather than definitive diagnostic markers, given known limitations of ratio-based and percentage-derived parameters. METHODS: This retrospective study included 72 women, equally allocated into four groups: lipoedema, FPLD, obesity, and normal-weight controls. All participants underwent whole-body DXA scanning to quantify total and regional fat parameters. Receiver operating characteristic (ROC) analyses assessed the discriminative performance of various indices. RESULTS: DXA revealed distinct adiposity patterns across groups. Lipoedema was best distinguished by a lower trunk fat mass (FM)/leg FM ratio (AUC: 0.964; p < 0.001). FPLD showed a centripetal fat distribution, notably via trunk FM/leg FM and trunk FM/limb FM ratios (both AUC: 0.860; p < 0.001). A total fat percentage >/=43.5% discriminated lipoedema from FPLD with near-perfect accuracy (AUC: 0.990). CONCLUSION: DXA-based assessment of regional fat distribution may provide supportive objective information for differentiating clinically selected women with lipoedema, FPLD, obesity, and normal adiposity; ratio-based and percentage-derived indices should be regarded as exploratory and hypothesis-generating rather than definitive diagnostic criteria. The applicability of these findings to male populations requires further investigation. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Avci, F AU - Avci F AUID- ORCID: 0009-0002-2392-2190 AD - Ege University School of Medicine, Department of Nuclear Medicine, Izmir, Turkey. FAU - Ibrahim, S AU - Ibrahim S AUID- ORCID: 0009-0001-7083-4583 AD - Ege University School of Medicine, Department of Physical Medicine and Rehabilitation, Izmir, Turkey. FAU - Simsek, A AU - Simsek A AUID- ORCID: 0009-0002-9568-6158 AD - Ege University School of Medicine, Izmir, Turkey. FAU - Argon, A M AU - Argon AM AUID- ORCID: 0000-0001-8572-9367 AD - Ege University School of Medicine, Department of Nuclear Medicine, Izmir, Turkey. FAU - Eyigor, S AU - Eyigor S AUID- ORCID: 0000-0002-9781-2712 AD - Ege University School of Medicine, Department of Physical Medicine and Rehabilitation, Izmir, Turkey. FAU - Simsir, I Y AU - Simsir IY AUID- ORCID: 0000-0002-6801-8499 AD - Ege University School of Medicine, Department of Internal Medicine, Division of Endocrinology and Metabolism Disorders, Izmir, Turkey. ilgin.yildirim.simsir@ege.edu.tr. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Endocrine JT - Endocrine JID - 9434444 SB - IM MH - Humans MH - Female MH - *Absorptiometry, Photon MH - *Obesity/diagnostic imaging/diagnosis MH - *Lipodystrophy, Familial Partial/diagnostic imaging/diagnosis MH - Adult MH - Retrospective Studies MH - Middle Aged MH - Diagnosis, Differential MH - *Lipedema/diagnostic imaging/diagnosis MH - Adiposity OTO - NOTNLM OT - Body composition assessment OT - Dual-energy X-ray absorptiometry OT - Lipodystrophy OT - Lipoedema OT - Obesity COIS- Declaration. Ethics approval: This study was conducted in accordance with the principles of the Declaration of Helsinki. The study protocol was approved by the Ege University Ethics Committee (approval no: 25-3 T/60; approved on 6 March 2025). Due to the retrospective design of the study, written informed consent was not required in accordance with local and national regulations. Conflict of interest: The authors declare no competing interests. Use of AI and AI-assisted Technologies: During the course of preparing this work, the authors used ChatGPT for the purpose of graphical abstract creation and to correct grammatical errors and standardise academic writing. No content was generated or interpreted by AI. Following the use of this tool/service, the authors formally reviewed the content for its accuracy and edited it as necessary. The authors take full responsibility for all the content of this publication. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:26 PHST- 2026/03/31 00:00 [received] PHST- 2026/07/14 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:26 [entrez] AID - 10.1007/s12020-026-04723-3 [pii] AID - 10.1007/s12020-026-04723-3 [doi] PST - epublish SO - Endocrine. 2026 Oct 29;91(1):355. doi: 10.1007/s12020-026-04723-3. PMID- 42955483 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2008-2231 (Electronic) IS - 1560-8115 (Linking) VI - 34 IP - 2 DP - 2026 Oct 29 TI - Combined vitamins C and E attenuate memory impairment and hippocampal damage in diabetic sleep-deprived mice: behavioral and molecular insights. LID - 100 [pii] LID - 10.1007/s40199-026-00677-z [doi] AB - BACKGROUND: Diabetes mellitus (DM) and sleep disturbances are associated with cognitive impairment and hippocampal dysfunction, potentially involving oxidative stress and neuroinflammation. OBJECTIVE: This study investigated the effects of vitamins C and E, individually and in combination, to determine whether their complementary antioxidant properties provide enhanced neuroprotective effects against cognitive and hippocampal alterations in diabetic sleep-deprived mice. METHODS: Diabetes was induced in male BALB/c mice (25-30 g) by streptozotocin (60 mg/kg), followed by partial sleep deprivation using the inverted flowerpot method. Animals received vitamin C (50 mg/kg), vitamin E (100 mg/kg), or their combination for 20 days. Body weight and blood glucose were monitored. Spatial learning and memory were evaluated using the Morris water maze. Hippocampal histopathology, GFAP immunostaining, and expression of genes related to oxidative stress, inflammation, and neuroprotection were assessed. RESULTS: Vitamin E and combined vitamin C and E treatment reduced FBG and were associated with higher final body weight than untreated diabetic sleep-deprived mice. Vitamin treatments improved spatial learning and memory, with combined treatment producing greater improvements in selected behavioral outcomes. Combined treatment increased hippocampal neuronal density and reduced GFAP immunoreactivity. Vitamin treatment increased BDNF, Nrf2, and HO-1 expression and reduced iNOS, TNF-alpha, and IL-1beta expression. CONCLUSION: Vitamin C and E, particularly in combination, were associated with improved metabolic, cognitive, and hippocampal outcomes in diabetic sleep-deprived mice, potentially involving modulation of neuroinflammatory and oxidative stress-related markers. CI - (c) 2026. The Author(s), under exclusive licence to Tehran University of Medical Sciences. FAU - Habibnia, Fatemeh AU - Habibnia F AD - Student Research Committee, Babol University of Medical Sciences, Babol, Iran, Islamic Republic of. FAU - Hamzeh, Olia AU - Hamzeh O AD - Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran, Islamic Republic of. FAU - Ashrafpour, Manochehr AU - Ashrafpour M AD - Mobility Impairment Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran, Islamic Republic of. FAU - Habibi, Danial AU - Habibi D AUID- ORCID: 0000-0003-4080-1809 AD - Social Determinants of Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran, Islamic Republic of. FAU - Pourabdolhossein, Fereshteh AU - Pourabdolhossein F AD - Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran, Islamic Republic of. pourabdolhossein@gmail.com. LA - eng GR - 724136046/Babol University of Medical Sciences/ PT - Journal Article DEP - 20261029 PL - Switzerland TA - Daru JT - Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences JID - 101125969 RN - PQ6CK8PD0R (Ascorbic Acid) RN - 1406-18-4 (Vitamin E) RN - 0 (Antioxidants) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Neuroprotective Agents) RN - EC 1.14.13.39 (Nitric Oxide Synthase Type II) RN - 0 (Interleukin-1beta) SB - IM MH - Animals MH - *Ascorbic Acid/pharmacology/administration & dosage MH - Male MH - *Hippocampus/drug effects/metabolism/pathology MH - *Sleep Deprivation/drug therapy/complications MH - *Vitamin E/pharmacology/administration & dosage MH - Mice MH - Mice, Inbred BALB C MH - *Diabetes Mellitus, Experimental/drug therapy/complications/metabolism MH - *Memory Disorders/drug therapy/metabolism/etiology MH - Maze Learning/drug effects MH - *Antioxidants/pharmacology/administration & dosage MH - Oxidative Stress/drug effects MH - Brain-Derived Neurotrophic Factor/metabolism/genetics MH - *Neuroprotective Agents/pharmacology/administration & dosage MH - Nitric Oxide Synthase Type II/metabolism/genetics MH - Interleukin-1beta/metabolism/genetics OTO - NOTNLM OT - Antioxidant OT - Cognitive dysfunction OT - Diabetes mellitus OT - Hippocampus OT - Neuroinflammation OT - Sleep deprivation COIS- Declarations. Ethics approval and consent to participate: A total of 64 male BALB/c mice (25-35 g, approximately 3 months old) were obtained from the Laboratory Animal Breeding Center of Babol University of Medical Sciences. The animals were housed under standard laboratory conditions at a temperature of 22 +/- 1 degrees C and relative humidity at 60%, with a 12-hour light/dark cycle. Food and water were available ad libitum throughout the study. All experimental procedures were reviewed and approved by the Ethics Committee of Babol University of Medical Sciences (Approval No: IR.MUBABOL.AEC.1403.011) and were conducted in accordance with institutional guidelines for the care and use of laboratory animal. Competing interests: The authors declare no competing interests. Authors statements: The article is not under consideration for publication elsewhere. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:26 PHST- 2026/05/15 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:26 [entrez] AID - 10.1007/s40199-026-00677-z [pii] AID - 10.1007/s40199-026-00677-z [doi] PST - epublish SO - Daru. 2026 Oct 29;34(2):100. doi: 10.1007/s40199-026-00677-z. PMID- 42955482 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1935-973X (Print) IS - 1935-9748 (Linking) VI - 19 IP - 1 DP - 2026 Oct 28 TI - Weightbearing CT in the Evaluation of Athletic Foot and Ankle Injuries. LID - 72 [pii] LID - 10.1007/s12178-026-10068-1 [doi] AB - PURPOSE OF REVIEW: Subtle foot and ankle injuries in athletes, including syndesmotic sprains, ligamentous Lisfranc injuries, osteochondral lesions of the talus, and proximal fifth metatarsal stress fractures, may be underestimated or misclassified on conventional imaging. This review summarizes the current role of weightbearing CT (WBCT) in their evaluation, focusing on quantitative parameters, recent technical advances, and their potential influence on clinical decision-making. RECENT FINDINGS: Recent evidence has shifted WBCT from qualitative assessment toward reproducible three-dimensional quantification. Volumetric assessment and distance mapping improve characterization of syndesmotic instability, while external-rotation stress can reveal abnormalities not evident during neutral standing. In the midfoot, triplanar realignment and side-to-side comparison improve the reliability of Lisfranc measurements and support detection of subtle instability. Distance mapping permits reproducible quantification of osteochondral lesion surface area, depth, and volume and may improve consistency in surgical planning. WBCT has also characterized sport-specific alignment patterns and a morphologic and bone-density phenotype associated with proximal fifth metatarsal stress fractures. Semiautomated segmentation and deep-learning methods may further reduce observer dependence and analysis time. WBCT provides three-dimensional measurements under physiologic load that complement clinical examination and conventional imaging in selected athletic foot and ankle conditions. Its greatest current value lies in characterizing subtle instability, malalignment, and osseous morphology when these findings may alter treatment planning. However, most available evidence is retrospective and derived from symptomatic or injured cohorts. Prospective studies are needed to validate diagnostic thresholds, determine whether WBCT-derived parameters predict injury or outcomes, and define their role in routine athletic screening and return-to-sport decision-making. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Brue, Julieta AU - Brue J AUID- ORCID: 0000-0001-8378-0863 AD - Department of Orthopaedic Surgery, Foot and Ankle Division, Duke University School of Medicine, Durham, NC, USA. julieta.brue@duke.edu. FAU - Micicoi, Lolita AU - Micicoi L AUID- ORCID: 0000-0001-6049-6632 AD - Department of Orthopaedic Surgery, Foot and Ankle Division, Duke University School of Medicine, Durham, NC, USA. AD - Institut Universitaire Locomoteur et du Sport du, CHU de Nice, 30 Voie Romaine, 06100, Nice, France. FAU - de Cesar Netto, Cesar AU - de Cesar Netto C AUID- ORCID: 0000-0001-6037-0685 AD - Department of Orthopaedic Surgery, Foot and Ankle Division, Duke University School of Medicine, Durham, NC, USA. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Curr Rev Musculoskelet Med JT - Current reviews in musculoskeletal medicine JID - 101317803 OTO - NOTNLM OT - Athletic injuries OT - Foot alignment OT - Foot and ankle OT - Sports injuries OT - Syndesmotic instability OT - Weight-bearing CT COIS- Declarations. Human and Animal Rights and Informed Consent: This article does not contain any studies with human or animal subjects performed by any of the authors. Competing Interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:26 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:26 [entrez] AID - 10.1007/s12178-026-10068-1 [pii] AID - 10.1007/s12178-026-10068-1 [doi] PST - epublish SO - Curr Rev Musculoskelet Med. 2026 Oct 28;19(1):72. doi: 10.1007/s12178-026-10068-1. PMID- 42955477 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2008-2231 (Electronic) IS - 1560-8115 (Linking) VI - 34 IP - 2 DP - 2026 Oct 28 TI - Virtual screening, synthesis, solid state characterization, evaluation for solubility, dissolution, and stability of gallic acid-based cocrystals of glimepiride. LID - 98 [pii] LID - 10.1007/s40199-026-00687-x [doi] AB - BACKGROUND: Glimepiride is a second generation Sulfonylurea class of antidiabetic drug. It belongs to BCS Class-II with a varying dissolution profile and bioavailability on account of limited aqueous solubility. The present study aimed to alter and improve physicochemical properties of glimepiride by modifying its crystal lattice through co-processing the same with an inert carrier like gallic acid to form cocrystals. OBJECTIVE: The present study deals with rational approach to select coformer for Co-crystallization of Glimepiride, followed by its confirmation by solid state characterization using FTIR, DSC and PXRD studies. Cocrystal formation and its effect on solubility, in-vitro drug release and stability were also evaluated. METHODS: Gallic acid was selected as coformer through multiple virtual screening methods such as COSMO-RS, Hansen solubility parameter (HSP), and pKa method. Cocrystal was formulated by solvent assisted grinding method using methanol and characterized by Fourier-transform spectroscopy (FTIR), Differential scanning calorimetry (DSC), Powder X-ray diffraction method (PXRD), and Solid state nuclear magnetic resonance spectroscopy (ssNMR) to identify the solid-state modification. Equilibrium solubility and in-vitro dissolution study were accomplished to estimate the solubility and dissolution behavior of cocrystals in comparison to pure drug. The drug content analysis of cocrystal formulation proved no loss of API during processing while stability study showed the integrity of drug in formulation throughout the study. RESULTS: Cocrystal formation of Glimepiride with gallic acid was confirmed by spectroscopic changes in FTIR and DSC. Comparative PXRD and ssNMR study further endorsed the formation of new crystalline species. Experimental confirmation of cocrystals have proved reliability of virtual screening methods. The seven-fold rise in aqueous solubility of cocrystal formulation than that of pure drug attributed to new crystalline phase with minimum lattice energy that further contributed to drug solubility and dissolution enhancement. CONCLUSION: Co-crystallization of Glimepiride with gallic acid is simple, reliable formulation approach to alter solubility and dissolution by altering crystal lattice of drug. Thus, co-crystallization is effective method for optimizing therapeutic efficacy of Glimepiride. CI - (c) 2026. The Author(s), under exclusive licence to Tehran University of Medical Sciences. FAU - Gandhi, Sanket M AU - Gandhi SM AD - Rajarambapu College of Pharmacy, Kasegaon, Tal. Walwa, Dist. Sangli, MS, 415404, India. sanket.gandhi21@gmail.com. AD - Shivaji University, Kolhapur, MS, 416004, India. sanket.gandhi21@gmail.com. AD - Department of Pharmaceutics, Tatyasaheb Kore college of Pharmacy, Warana University, Warananagar, Tal. Panhala, Dist. Kolhapur, MS, 416113, India. sanket.gandhi21@gmail.com. FAU - Mohite, Shrinivas K AU - Mohite SK AD - Rajarambapu College of Pharmacy, Kasegaon, Tal. Walwa, Dist. Sangli, MS, 415404, India. mohite_shrinivas@rediffmail.com. AD - Shivaji University, Kolhapur, MS, 416004, India. mohite_shrinivas@rediffmail.com. FAU - Shete, Amol S AU - Shete AS AUID- ORCID: 0000-0003-0350-432X AD - Department of Pharmaceutics, Krishna Institute of Pharmacy, Krishna Vishwa Vidyapeeth (Deemed to be University), Karad, Dist. Satara, MS, 415539, India. FAU - Dol, Hemalata S AU - Dol HS AD - Department of Pharmaceutics, Krishna Institute of Pharmacy, Krishna Vishwa Vidyapeeth (Deemed to be University), Karad, Dist. Satara, MS, 415539, India. FAU - Nagvekar, Utkarsh V AU - Nagvekar UV AD - Department of Pharmaceutical Chemistry, Vasantidevi Patil Institute of Pharmacy, Kodoli, Tal. Panhala, Dist. Kolhapur, MS, 416114, India. FAU - Desai, Anand V AU - Desai AV AD - Department of Pharmacology, Krishna Institute of Pharmacy, Krishna Vishwa Vidyapeeth (Deemed to be University), Karad, Dist. Satara, MS, 415539, India. FAU - Disouza, John I AU - Disouza JI AD - Department of Pharmaceutics, Bombay Institute of Pharmacy and Research, Dombivali (East), Dist. Thane, MS, 421201, India. LA - eng PT - Journal Article DEP - 20261028 PL - Switzerland TA - Daru JT - Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences JID - 101125969 RN - 6KY687524K (glimepiride) RN - 0 (Sulfonylurea Compounds) RN - 632XD903SP (Gallic Acid) RN - 0 (Hypoglycemic Agents) RN - 0 (Bulk Drugs) SB - IM MH - *Sulfonylurea Compounds/chemistry MH - *Gallic Acid/chemistry MH - Solubility MH - Crystallization MH - Spectroscopy, Fourier Transform Infrared MH - Drug Stability MH - Calorimetry, Differential Scanning MH - *Hypoglycemic Agents/chemistry MH - X-Ray Diffraction MH - Drug Liberation MH - Bulk Drugs OTO - NOTNLM OT - Cocrystals OT - Gallic acid OT - Glimepiride OT - Solubility enhancement OT - Solvent assisted grinding COIS- Declarations. Ethics approval and consent to participate: Not Applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:26 PHST- 2026/03/09 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:26 [entrez] AID - 10.1007/s40199-026-00687-x [pii] AID - 10.1007/s40199-026-00687-x [doi] PST - epublish SO - Daru. 2026 Oct 28;34(2):98. doi: 10.1007/s40199-026-00687-x. PMID- 42955475 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1573-4978 (Electronic) IS - 0301-4851 (Linking) VI - 53 IP - 1 DP - 2026 Oct 29 TI - Molecular mechanisms, functional diversity, and therapeutic implications of HSP90 isoforms in lung cancer. LID - 1850 [pii] LID - 10.1007/s11033-026-13089-8 [doi] AB - Heat shock protein 90 (HSP90) is a critical molecular chaperone that ensures the stability and maturation of oncogenic client proteins in lung cancer. This review examines the distinct roles of HSP90alpha, HSP90beta, GRP94 and the mitochondrial paralog TRAP1 in driving disease progression. While HSP90alpha governs invasiveness and metastasis, HSP90beta maintains homeostatic oncogenic signalling. TRAP1 serves as a metabolic lynchpin, facilitating metabolic reprogramming, mitochondrial cytoprotection, and resistance to apoptosis. Together, these isoforms stabilize key drivers such as EGFR, ALK and KRAS, fostering therapeutic resistance. Utilizing systems biology, bioinformatics and comparative analysis of experimental studies, this review explores the high-throughput identification of HSP90 isoforms as central "hub genes" within the lung cancer interactome. The therapeutic potential of mitochondrial-targeted drug delivery systems and isoform-selective inhibitors designed to minimize systemic toxicity are explored in detail. Ultimately, understanding isoform-specific dynamics and multi-omics regulatory networks is essential for developing personalized, selective interventions to overcome drug resistance and improve lung cancer management. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature B.V. FAU - Janani, K P AU - Janani KP AD - Department of Biotechnology, Sri Venkateswara College of Engineering, Pennalur, Tamil Nadu, 602117, Sriperumbudur Tk, India. FAU - Lalithammbika, S K AU - Lalithammbika SK AD - Department of Biotechnology, Sri Venkateswara College of Engineering, Pennalur, Tamil Nadu, 602117, Sriperumbudur Tk, India. FAU - Praveen Kumar, P K AU - Praveen Kumar PK AUID- ORCID: 0000-0002-8915-3542 AD - Department of Biotechnology, Sri Venkateswara College of Engineering, Pennalur, Tamil Nadu, 602117, Sriperumbudur Tk, India. praveenpk@svce.ac.in. AD - Phytonanos Drug Technologies Pvt. Ltd, Chennai, 600073, Tamil Nadu, India. praveenpk@svce.ac.in. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - Netherlands TA - Mol Biol Rep JT - Molecular biology reports JID - 0403234 RN - 0 (HSP90 Heat-Shock Proteins) RN - 0 (Protein Isoforms) RN - 0 (TRAP1 protein, human) SB - IM MH - Humans MH - *HSP90 Heat-Shock Proteins/metabolism/genetics MH - *Lung Neoplasms/metabolism/genetics/drug therapy/pathology MH - Protein Isoforms/metabolism/genetics MH - Mitochondria/metabolism MH - Animals MH - Signal Transduction MH - Gene Expression Regulation, Neoplastic MH - Drug Resistance, Neoplasm OTO - NOTNLM OT - HSP90 Isoforms OT - Mitochondrial-targeted drug delivery OT - Multi-omics OT - TRAP1 COIS- Declarations. Competing interests: The authors declare no competing interests. Use of Generative AI or Software in Image Preparation: ChatGPT, Nanobanana and Gpai were used solely as AI-assisted visualisation and graphic-design tools to develop schematic representations of concepts and mechanisms described in this manuscript. The authors determined and verified the scientific content, molecular interactions, pathway relationships, interpretation, and final figure design. No experimental data were generated or modified using AI. The authors assume full responsibility for the accuracy and scientific integrity of the final figures. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:25 PHST- 2026/07/11 00:00 [received] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:25 [entrez] AID - 10.1007/s11033-026-13089-8 [pii] AID - 10.1007/s11033-026-13089-8 [doi] PST - epublish SO - Mol Biol Rep. 2026 Oct 29;53(1):1850. doi: 10.1007/s11033-026-13089-8. PMID- 42955471 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1573-2649 (Electronic) IS - 0962-9343 (Linking) VI - 35 IP - 11 DP - 2026 Oct 28 TI - Measurement properties of the EQ-5D-5L and SF-6D in measuring the health-related quality of life of non-small cell lung cancer patients on active treatments in China. LID - 316 [pii] LID - 10.1007/s11136-026-04402-w [doi] AB - BACKGROUND: Lung cancer is the leading cause of cancer-related death globally, including China, with non-small cell lung cancer (NSCLC) accounting for 85% of cases. Novel NSCLC drugs need cost-utility analysis (CUA) for Chinese reimbursement. CUA relies on health utility values, but the only measures with value sets for Chinese populations, i.e., EQ-5D-5L and SF-6D, lack NSCLC-specific reliability and validity comparison evidence. This study examined their measurement properties in Chinese patients with NSCLC. METHODS: A cross-sectional survey was conducted across 18 provincial-level jurisdictions in China (2022.10-2024.03) with 947 patients from 20 hospitals. EQ-5D-5L, SF-6D, and QLQ-C30 were administered to patients, and a total of 886 patients with complete responses to all three aforementioned measures were included in the analysis of this study. Measurement properties were evaluated via ceiling/floor effects, convergent validity (with QLQ-C30), known-groups validity (gender, age, cancer stage, surgery, brain metastasis, bone metastasis, and COVID-19 infection history), and Shannon (H') and evenness (J') indices. RESULTS: Among the analysis sample (n = 886), participants were 36.5% female with a mean age of 61.5 years and a mean disease duration of 1.8 years; 64.1% had stage IV disease. Ceiling effects for the EQ-5D-5L and SF-6D were 26.07% and 3.50%, respectively. Both tools showed moderate-to-strong correlations with relevant QLQ-C30 dimensions (|rho| = 0.58-0.84, all p < 0.001). For known-groups validity, small-to-medium effect sizes were found for subgroups of age, cancer stage, operation, and bone metastasis for EQ-5D-5L; and cancer stage, operation, bone metastasis, and COVID-19 infection history for SF-6D. Regarding informativity, H'/J' ranged from 1.061/0.457 for "Self-care" to 1.737/0.748 for "Pain/discomfort" for EQ-5D-5L, and 1.925/0.829 for "mental health" to 2.223/0.957 for "social functioning" for SF-6D. CONCLUSIONS: Both the EQ-5D-5L and SF-6D have robust measurement properties for assessing health status in Chinese NSCLC patients. CI - (c) 2026. The Author(s). FAU - Wang, Jiatai AU - Wang J AD - Qi-Huang Chinese Medicine School, Beijing University of Chinese Medicine, Beijing, China. FAU - Suo, Yusi AU - Suo Y AD - Center for Evidence-Based Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China. AD - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China. FAU - Wang, Yue AU - Wang Y AD - School of International Pharmaceutical Business, China Pharmaceutical University, Nanjing, China. FAU - Li, Yiwei AU - Li Y AD - Center for Evidence-Based Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China. AD - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China. FAU - Wang, Luying AU - Wang L AD - School of International Pharmaceutical Business, China Pharmaceutical University, Nanjing, China. FAU - Guan, Xin AU - Guan X AD - School of International Pharmaceutical Business, China Pharmaceutical University, Nanjing, China. FAU - Guo, Yuanyuan AU - Guo Y AD - Cancer Center, Inner Mongolia Autonomous Region Hospital of Traditional Chinese Medicine, Hohhot, China. FAU - Yang, Ming AU - Yang M AD - Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, China. FAU - Wang, Hanfei AU - Wang H AD - Center for Evidence-Based Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China. AD - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China. FAU - Zhu, Haotian AU - Zhu H AD - Qi-Huang Chinese Medicine School, Beijing University of Chinese Medicine, Beijing, China. FAU - Liang, Wanxian AU - Liang W AD - Traditional Chinese Medicine Department, Longgang Central Hospital of Shenzhen, Shenzhen, China. FAU - Song, Na AU - Song N AD - Department of Oncology, Chongqing Traditional Chinese Medicine Hospital, Chongqing, China. FAU - Xu, Ying AU - Xu Y AD - Respiratory Department, Heilongjiang Provincial Hospital, Harbin, China. FAU - Wang, Jia AU - Wang J AD - Third Affiliated Hospital, Beijing University of Chinese Medicine, Beijing, China. FAU - Tang, Lin AU - Tang L AD - Department of Pharmacy, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China. FAU - Ye, Ziqi AU - Ye Z AD - The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. FAU - Xie, Feng AU - Xie F AD - Department of Health Research Methods, Evidence, and Impact, Faculty of Health Sciences, McMaster University, Hamilton, Canada. AD - Centre for Health Economics and Policy Analysis, Faculty of Health Sciences, McMaster University, Hamilton, Canada. FAU - Li, Hongchao AU - Li H AD - School of International Pharmaceutical Business, China Pharmaceutical University, Nanjing, China. lihongchao@cpu.edu.cn. FAU - Jin, Xuejing AU - Jin X AD - Center for Evidence-Based Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China. jinxuejing@bucm.edu.cn. AD - School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China. jinxuejing@bucm.edu.cn. LA - eng GR - 318-RA/the EuroQol Research Foundation/ PT - Journal Article DEP - 20261028 PL - Netherlands TA - Qual Life Res JT - Quality of life research : an international journal of quality of life aspects of treatment, care and rehabilitation JID - 9210257 SB - IM MH - Humans MH - Female MH - *Carcinoma, Non-Small-Cell Lung/psychology/therapy MH - *Quality of Life/psychology MH - China MH - *Lung Neoplasms/psychology/therapy MH - Middle Aged MH - Male MH - Cross-Sectional Studies MH - Aged MH - Surveys and Questionnaires/standards MH - Reproducibility of Results MH - Psychometrics MH - Adult OAB - In China, economic evaluations for new drug reimbursement require reliable health utility data. This study was motivated by the lack of evidence on the measurement properties of the two primary Chinese preference-based measures-the EQ-5D-5L and SF-6D-in patients with non-small cell lung cancer (NSCLC). We aimed to address this gap by systematically comparing their performance to inform their suitability for cost-utility analysis. A cross-sectional survey of 947 NSCLC patients from 20 Chinese hospitals was conducted. After screening, 886 participants who had completed the EQ-5D-5L, SF-6D, and the QLQ-C30 were obtained. We evaluated response patterns, construct validity, known-groups validity, and informativity. Both instruments demonstrated robust and comparable measurement properties for assessing HRQoL in Chinese NSCLC patients. A key difference was the significantly higher ceiling effect for the EQ-5D-5L (26.07%) versus the SF-6D (3.50%). Both measures showed moderate-to-strong correlations with relevant QLQ-C30 domains, supporting construct validity. Known-groups validity was confirmed, as both effectively distinguished patients across clinical subgroups such as cancer stage and surgery status. Overall, the SF-6D exhibited marginally better informativity and discriminatory power across its dimensions. In conclusion, both the EQ-5D-5L and SF-6D are valid for deriving health utilities in Chinese NSCLC patients and are suitable for economic evaluations. The selection between them may involve weighing their relative strengths regarding ceiling effects and validated performance. These results provide essential evidence for health economic research and policy-making regarding NSCLC treatment in China. OABL- eng OTO - NOTNLM OT - EQ-5D-5L OT - Measurement properties OT - NSCLC OT - QLQ-C30 OT - SF-6D COIS- Declarations. Conflict of interest: Feng Xie and Xuejing Jin are members of the EuroQol Group. Other authors declare they have no conflict of interests. Ethical approval: Ethics approval (SECCR/2021-192-01) was obtained from the Shanghai Ethics Committee for Clinical Research. Consent to participate: Informed consent was obtained from all individual participants included in the study. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:25 PHST- 2025/12/09 00:00 [received] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:25 [entrez] AID - 10.1007/s11136-026-04402-w [pii] AID - 10.1007/s11136-026-04402-w [doi] PST - epublish SO - Qual Life Res. 2026 Oct 28;35(11):316. doi: 10.1007/s11136-026-04402-w. PMID- 42955467 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1522-1709 (Electronic) IS - 1520-9512 (Linking) VI - 30 IP - 6 DP - 2026 Oct 29 TI - The role of DISE and simulation bite in the selection of non-CPAP treatment modalities in patients with OSA. LID - 298 [pii] LID - 10.1007/s11325-026-03846-1 [doi] AB - PURPOSE: This study aims to investigate the role of drug-induced sleep endoscopy (DISE) as a predictor of success of mandibular repositioning appliances (MRA), tonsillectomy and modified uvulopalatopharyngoplasty (mUPPP). Furthermore, the potential benefit of the use of a simulation bite during DISE is evaluated. METHODS: A retrospective study was performed on 82 obstructive sleep apnoea (OSA) patients unwilling or not tolerating continuous positive airway pressure (CPAP) treatment. Patients with large tonsils were offered tonsillectomy and were excluded from the study. Nasal pathology was treated before possible inclusion in the study. All patients underwent DISE with chin lift or simulation bite to predict the effect of MRA, mUPPP and tonsillectomy and underwent a post-treatment polysomnography (PSG) afterwards. RESULTS: Of the 56 patients that were advised to start treatment with MRA and underwent post-treatment PSG, 96% achieved success. Only 4 patients underwent tonsillectomy and 7 underwent mUPPP, both with lower success rates (50% and 29% respectively). There was no difference in success rates of MRA between patients undergoing DISE with simulation bite or chin lift. CONCLUSION: The study confirms that DISE is an excellent predictor of MRA success. The success rate of mUPPP or tonsillectomy in cases of small tonsils is much lower than that of MRA and DISE is a less effective predictor of success for these surgical procedures.The value of using a simulation bite during DISE cannot be confirmed by this study. An adequately applied chin lift seems to predict the therapeutic effect of MRA as well as the use of a simulation bite. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature Switzerland AG. FAU - Cuypers, Sien AU - Cuypers S AUID- ORCID: 0009-0003-5257-3804 AD - Department of Otolaryngology-Head and Neck Surgery, UZ Leuven, Leuven, Belgium. sien.cuypers@student.kuleuven.be. FAU - De Ferm, Alexe AU - De Ferm A AUID- ORCID: 0009-0006-8719-3037 AD - Department of Otolaryngology-Head and Neck Surgery, UZ Leuven, Leuven, Belgium. FAU - Jacobs, Raf AU - Jacobs R AD - Department of Otolaryngology-Head and Neck Surgery, AZ Sint Blasius, Dendermonde, Belgium. LA - eng PT - Journal Article DEP - 20261029 PL - Germany TA - Sleep Breath JT - Sleep & breathing = Schlaf & Atmung JID - 9804161 SB - IM MH - Humans MH - *Sleep Apnea, Obstructive/therapy/diagnosis MH - Female MH - Retrospective Studies MH - Male MH - Polysomnography MH - *Endoscopy/methods MH - Tonsillectomy MH - Adult MH - *Mandibular Advancement/instrumentation MH - Continuous Positive Airway Pressure MH - Middle Aged MH - Treatment Outcome MH - Uvula/surgery OTO - NOTNLM OT - Drug-induced sleep endoscopy OT - MAD OT - MRA OT - Obstructive sleep apnea OT - Simulation bite COIS- Declarations. Ethical approval: All procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional research committee (AZ Sint Blasius) and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. For this type of study formal consent is not required. Conflict of interest: All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers' bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:25 PHST- 2026/01/25 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/08/20 00:00 [revised] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:25 [entrez] AID - 10.1007/s11325-026-03846-1 [pii] AID - 10.1007/s11325-026-03846-1 [doi] PST - epublish SO - Sleep Breath. 2026 Oct 29;30(6):298. doi: 10.1007/s11325-026-03846-1. PMID- 42955454 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1573-7233 (Electronic) IS - 0167-7659 (Linking) VI - 45 IP - 4 DP - 2026 Oct 29 TI - Epigenetic messengers: extracellular vesicles at the interface of cancer communication. LID - 83 [pii] LID - 10.1007/s10555-026-10395-2 [doi] AB - Extracellular vesicles (EVs) have emerged as potent conveyors of epigenetic information, reshaping our understanding of intercellular communication in both health and disease. Beyond their established roles in transferring proteins and nucleic acids, EVs carry DNA methyltransferases and methylated DNA and RNA species, providing a dynamic mechanism for remodelling the methylome of recipient cells. In normal physiology, this exchange fine-tunes gene expression, development, and tissue homeostasis. However, under pathological conditions, particularly in cancer, EV-mediated transfer of methylation machinery becomes dysregulated, driving epigenetic reprogramming of the tumour microenvironment and promoting malignant progression. Tumour-derived EVs mirror the methylation landscape of their parent cells, positioning them as promising non-invasive biomarkers for early detection and disease monitoring. Despite this potential, significant variability in EV isolation, characterisation, and functional assessment continues to obscure mechanistic insights. Clarifying how specific EV cargo drives targeted methylation changes will be essential to harness their diagnostic and therapeutic utility. This review highlights the emerging concept of EVs as mobile epigenetic regulators, underscoring the need for standardisation and translational integration to fully exploit their promise in precision cancer medicine. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Duffy, Kate E AU - Duffy KE AD - School of Biology and Environmental Science, O'Brien Science Centre, University College Dublin, Dublin 4, Belfield, , Ireland. AD - Cancer Biology and Therapeutics Laboratory, UCD Conway Institute of Biomedical and Biomolecular Research, University College Dublin, Dublin, Ireland. FAU - McGee, Margaret M AU - McGee MM AD - School of Biomolecular and Biomedical Science, University College Dublin, Dublin, Ireland. FAU - Perry, Antoinette S AU - Perry AS AUID- ORCID: 0000-0002-6108-512X AD - School of Biology and Environmental Science, O'Brien Science Centre, University College Dublin, Dublin 4, Belfield, , Ireland. antoinette.perry@ucd.ie. AD - Cancer Biology and Therapeutics Laboratory, UCD Conway Institute of Biomedical and Biomolecular Research, University College Dublin, Dublin, Ireland. antoinette.perry@ucd.ie. LA - eng GR - ICRS24DUF/Irish Cancer Society/ PT - Journal Article PT - Review DEP - 20261029 PL - Netherlands TA - Cancer Metastasis Rev JT - Cancer metastasis reviews JID - 8605731 SB - IM MH - Humans MH - *Extracellular Vesicles/metabolism/genetics MH - *Neoplasms/genetics/pathology/metabolism MH - *Epigenesis, Genetic MH - Animals MH - Cell Communication MH - DNA Methylation MH - Tumor Microenvironment OTO - NOTNLM OT - DNA methylation OT - DNA methyltransferases OT - Epigenetics OT - Extracellular vesicles OT - Tumour microenvironment COIS- Declarations. Ethics approval and consent to participate: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:23 PHST- 2026/07/15 00:00 [received] PHST- 2026/10/24 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:23 [entrez] AID - 10.1007/s10555-026-10395-2 [pii] AID - 10.1007/s10555-026-10395-2 [doi] PST - epublish SO - Cancer Metastasis Rev. 2026 Oct 29;45(4):83. doi: 10.1007/s10555-026-10395-2. PMID- 42955453 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1573-6768 (Electronic) IS - 1389-5729 (Linking) VI - 27 IP - 6 DP - 2026 Oct 29 TI - Beyond age prediction: a task-oriented framework for evaluating molecular aging clocks. LID - 190 [pii] LID - 10.1007/s10522-026-10535-9 [doi] AB - Biological age estimation is increasingly used in aging research, but evaluation often relies too heavily on mean absolute error (MAE) against chronological age. We conducted a narrative review of major molecular aging clocks and propose an operational framework that links clock selection to the intended task. Applications are organized into four tasks: chronological-age estimation, age-independent deviation, future disease or mortality risk, and longitudinal monitoring of aging pace or intervention response. For each task, the framework specifies the target construct, output, preprocessing and confounding checks, validation metrics, and interpretation limits. A complementary four-layer model distinguishes relatively stable molecular measures, pace-of-aging measures, dynamic functional markers, and organ-specific vulnerability. Published examples illustrate why no single metric is sufficient: low age-prediction error does not establish clinical relevance, and strong risk association does not establish responsiveness to intervention. Aging clocks should therefore be evaluated as task-bound measurement tools, with explicit attention to calibration, discrimination, reliability, transportability, uncertainty, and incremental value. This framework is intended to support transparent model selection, reporting, and interpretation rather than designate a universally superior clock. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature B.V. FAU - Simayijiang, Halimureti AU - Simayijiang H AUID- ORCID: 0009-0000-1786-4049 AD - South China Hospital, Medical School, Shenzhen University, Shenzhen, People's Republic of China. halimureti@qq.com. FAU - Ye, Pei AU - Ye P AD - Department of Hyperbaric Oxygen, Shenzhen People's Hospital, Shenzhen, People's Republic of China. FAU - Zhang, Liuli AU - Zhang L AD - Department of Hyperbaric Oxygen, Shenzhen People's Hospital, Shenzhen, People's Republic of China. FAU - Wu, Jiacheng AU - Wu J AD - Department of Hyperbaric Oxygen, Shenzhen People's Hospital, Shenzhen, People's Republic of China. FAU - Li, Zhuo AU - Li Z AD - Department of Hyperbaric Oxygen, Shenzhen People's Hospital, Shenzhen, People's Republic of China. FAU - Long, Ying AU - Long Y AD - Department of Hyperbaric Oxygen, Shenzhen People's Hospital, Shenzhen, People's Republic of China. longying@szhospital.com. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - Netherlands TA - Biogerontology JT - Biogerontology JID - 100930043 SB - IM MH - Humans MH - *Aging/genetics/physiology/metabolism MH - Animals MH - *Biological Clocks OTO - NOTNLM OT - Age acceleration OT - Aging clock OT - Biological age OT - DNA methylation OT - DunedinPACE OT - Healthspan OT - Intervention response OT - Proteomic clock COIS- Declarations. Conflict of interest: The authors declare that they have no competing interests. Ethical approval Ethical approval was not required because this article is a narrative review and does not report new human participant or animal research. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:23 PHST- 2026/08/15 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:23 [entrez] AID - 10.1007/s10522-026-10535-9 [pii] AID - 10.1007/s10522-026-10535-9 [doi] PST - epublish SO - Biogerontology. 2026 Oct 29;27(6):190. doi: 10.1007/s10522-026-10535-9. PMID- 42955451 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1573-5028 (Electronic) IS - 0167-4412 (Linking) VI - 116 IP - 6 DP - 2026 Oct 28 TI - Transcriptomic responses of sugarcane to drought stress are modulated by bacterial synthetic communities. LID - 108 [pii] LID - 10.1007/s11103-026-01773-z [doi] AB - Drought intensification due to climate change poses a significant constraint on global sugarcane production, as the crop requires substantial water for optimal growth. Synthetic Communities (SynComs) have the potential to enhance drought resilience, yet their application and mechanisms of action remain unclear, particularly in sugarcane. In this study, transcriptomic analysis was employed to investigate how SynComs modulate responses to drought stress in sugarcane. Five potential SynComs (SC) were evaluated, and SC3 was selected for detailed gene expression analysis based on its relatively higher microbial diversity and community evenness as indicated by the Shannon diversity and Pielou's evenness indices, together with its ability to promote sugarcane growth and physiological processes. SC3 was associated with increased plant height, phosphorus uptake, and osmoprotectant content compared with the other SynComs. Transcriptomic analysis revealed differences in signal transduction: in SC3-inoculated plants, MAPK signaling was prominent, whereas in control plants, signaling relied more on plant hormones. Additionally, differences in energy allocation and secondary metabolite production as defense responses to drought stress were observed. SC3-treated plants allocated energy to maintaining lipid membrane integrity and lignification, while control plants focused on antioxidant activity via flavonoids. These results indicate that bacterial SynCom inoculants can modulate drought stress responses. Overall, the findings highlight the potential of SynComs as a sustainable microbial strategy for enhancing crop performance under drought stress. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature B.V. FAU - Sulastri, Sulastri AU - Sulastri S AUID- ORCID: 0000-0002-4570-6491 AD - Research Centre for Applied Microbiology, National Research and Innovation Agency (BRIN), Bogor, Indonesia. sula010@brin.go.id. FAU - Rahmawati, Rika Sri AU - Rahmawati RS AUID- ORCID: 0000-0003-0662-5055 AD - Research Centre for Applied Microbiology, National Research and Innovation Agency (BRIN), Bogor, Indonesia. FAU - Herliana, Lina AU - Herliana L AUID- ORCID: 0000-0001-5784-6654 AD - Research Centre for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor, Indonesia. FAU - Roswanjaya, Yuda Purwana AU - Roswanjaya YP AUID- ORCID: 0000-0002-8195-8100 AD - Research Centre for Applied Microbiology, National Research and Innovation Agency (BRIN), Bogor, Indonesia. FAU - Sukmadi, R Bambang AU - Sukmadi RB AUID- ORCID: 0009-0009-3148-4291 AD - Research Centre for Applied Microbiology, National Research and Innovation Agency (BRIN), Bogor, Indonesia. FAU - Asiani, Nia AU - Asiani N AUID- ORCID: 0009-0008-1757-2829 AD - Research Centre for Applied Microbiology, National Research and Innovation Agency (BRIN), Bogor, Indonesia. FAU - Irawati, Ana Feronika Cindra AU - Irawati AFC AUID- ORCID: 0000-0002-1582-9255 AD - Research Centre for Applied Microbiology, National Research and Innovation Agency (BRIN), Bogor, Indonesia. FAU - Saryanah, Nur Alfi AU - Saryanah NA AUID- ORCID: 0000-0003-1726-6924 AD - Research Centre for Applied Microbiology, National Research and Innovation Agency (BRIN), Bogor, Indonesia. FAU - Sedijani, Prapti AU - Sedijani P AUID- ORCID: 0000-0002-7568-772X AD - Department of Biology Education, Mataram University, Mataram, Indonesia. LA - eng GR - RIIM Kompetisi Gelombang 3/Indonesia Endowment Fund for Education Agency/ PT - Journal Article DEP - 20261028 PL - Netherlands TA - Plant Mol Biol JT - Plant molecular biology JID - 9106343 SB - IM MH - *Saccharum/microbiology/genetics/physiology/metabolism MH - *Droughts MH - *Stress, Physiological/genetics MH - Gene Expression Regulation, Plant MH - Drought Resistance MH - *Transcriptome MH - Gene Expression Profiling MH - *Bacteria/metabolism MH - Signal Transduction OTO - NOTNLM OT - Flavonoid OT - Lignin OT - Lipid membrane OT - MAPK OT - P absorption OT - Water deficit COIS- Declarations. Conflict of interest: All authors declare no conflict of interest. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:23 PHST- 2026/07/15 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:23 [entrez] AID - 10.1007/s11103-026-01773-z [pii] AID - 10.1007/s11103-026-01773-z [doi] PST - epublish SO - Plant Mol Biol. 2026 Oct 28;116(6):108. doi: 10.1007/s11103-026-01773-z. PMID- 42955447 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1573-7446 (Electronic) IS - 0165-7380 (Linking) VI - 51 IP - 1 DP - 2026 Oct 29 TI - Concurrent detection of low-pathogenic H9N2 avian influenza virus and Salmonella spp. in a poorly performing commercial layer flock in Bangladesh. LID - 25 [pii] LID - 10.1007/s11259-026-11587-5 [doi] AB - Avian influenza virus (AIV) remains a major threat to poultry production in Bangladesh, where both highly pathogenic (HPAIV) and low-pathogenic (LPAIV) subtypes circulate. Although HPAIV is typically associated with high mortality, H9N2 LPAIV often causes mild clinical disease but has been associated with reduced egg production and economic losses in commercial poultry. This study describes the concurrent detection of H9N2 and Salmonella spp. in a 33-week-old Hy-Line Brown commercial layer flock in Moulvibazar, Bangladesh, in which a marked decline in laying performance was observed. A total of 50 birds were sampled for virological and bacteriological investigations. Oropharyngeal swabs were tested by real-time reverse transcription PCR (rRT-PCR) targeting the influenza A virus matrix (M) gene, followed by H9- and N2-specific assays. Cloacal swabs were cultured using selective enrichment and plating methods, and presumptive Salmonella spp. isolates were confirmed by PCR targeting the invA gene. Among the sampled birds, concurrent H9N2 and Salmonella spp. were detected in 11/50, while no birds were positive for H9N2 alone; Salmonella spp. alone was detected in 3/50 birds. The investigated flock exhibited markedly reduced egg production, remaining at 0% through 25 weeks of age and reaching a maximum of only 71.72% at 46 weeks, compared with the breed-standard value of 88%, while cumulative mortality reached 23.4% by 46 weeks, substantially exceeding the breed standard. These findings document the concurrent detection of H9N2 and Salmonella spp. and poor laying performance in the investigated flock. Given the single-flock design, absence of an H9N2-only comparison group and a contemporaneous healthy control flock, and incomplete assessment of other infectious and farm-level factors, the findings do not establish a causal relationship or determine whether Salmonella spp. contributed to the observed production losses. Further controlled and longitudinal studies involving multiple flocks and appropriate comparison groups are warranted to clarify the respective contributions of H9N2 and bacterial coinfections to production losses in commercial layer flocks. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature B.V. FAU - Kayesh, Mohammad Enamul Hoque AU - Kayesh MEH AUID- ORCID: 0000-0003-4806-9843 AD - Department of Microbiology and Public Health, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barishal, 8210, Bangladesh. mehkayesh@pstu.ac.bd. FAU - Akimul, Md Kamruzzaman AU - Akimul MK AD - Department of Microbiology and Public Health, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barishal, 8210, Bangladesh. FAU - Hosen, Mosharaf AU - Hosen M AD - Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barishal, 8210, Bangladesh. FAU - Anower, A K M M AU - Anower AKMM AD - Department of Microbiology and Public Health, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University, Barishal, 8210, Bangladesh. FAU - Karim, Md Rezaul AU - Karim MR AUID- ORCID: 0000-0002-9046-3759 AD - Bangladesh Livestock Research Institute, Savar, 1341, Dhaka, Bangladesh. rezavetmicro@yahoo.com. LA - eng PT - Journal Article DEP - 20261029 PL - Switzerland TA - Vet Res Commun JT - Veterinary research communications JID - 8100520 SB - IM MH - Animals MH - *Influenza A Virus, H9N2 Subtype/isolation & purification/pathogenicity MH - Bangladesh/epidemiology MH - *Influenza in Birds/virology/epidemiology/microbiology MH - Female MH - *Chickens MH - *Salmonella Infections, Animal/epidemiology/microbiology/virology MH - *Salmonella/isolation & purification MH - *Poultry Diseases/virology/microbiology/epidemiology MH - *Coinfection/veterinary/virology/microbiology/epidemiology OTO - NOTNLM OT - Salmonella spp. OT - Bangladesh OT - Concurrent detection OT - Egg production OT - H9N2 avian influenza virus OT - Layer chickens COIS- Declarations. Ethics approval: The field sampling was conducted in September 2024 as part of a veterinary field investigation into an ongoing health and production problem in a commercial layer flock under the supervision of a private veterinary practitioner. The investigation was not initiated as a pre-planned research project. The collected diagnostic data were subsequently considered for scientific reporting. The study protocol was reviewed and approved in 2026 by the Institutional Ethical Committee of Patuakhali Science and Technology University, Bangladesh (Approval No. PSTU/IEC/70(i);10.06.2026). Competing interests: The authors declare no competing interests. Conflict of interest: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:22 PHST- 2026/08/03 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:22 [entrez] AID - 10.1007/s11259-026-11587-5 [pii] AID - 10.1007/s11259-026-11587-5 [doi] PST - epublish SO - Vet Res Commun. 2026 Oct 29;51(1):25. doi: 10.1007/s11259-026-11587-5. PMID- 42955436 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1573-2630 (Electronic) IS - 0165-5701 (Linking) VI - 46 IP - 1 DP - 2026 Oct 29 TI - Decreased superficial retinal microvascular density in alopecia areata: an OCT angiography study. LID - 446 [pii] LID - 10.1007/s10792-026-04327-w [doi] AB - PURPOSE: This study aimed to evaluate retinal microvascular and structural changes in patients with alopecia areata (AA) using optical coherence tomography angiography (OCTA). To our knowledge, retinal microvascular alterations have not been investigated via OCTA in AA before. METHODS: This prospective study included 40 patients with newly diagnosed AA and 42 healthy controls. Superficial capillary plexus vessel densities (VD-SCP) and deep capillary plexus vessel densities (VD-DCP), choriocapillaris flow area, foveal avascular zone (FAZ), retinal nerve fiber layer (RNFL) thickness, optic disc vascular parameters, and central corneal thickness (CCT) were measured. RESULTS: VD-SCP was significantly reduced in the whole image (p = 0.001), parafoveal (p = 0.001), and perifoveal regions (p = 0.007) in patients with AA compared with controls. These differences remained significant after FDR correction (adjusted p = 0.011, 0.011, and 0.049, respectively). However, foveal VD-SCP did not differ significantly (p = 0.221). An unadjusted difference in inferior RNFL thickness (p = 0.044) did not remain statistically significant after FDR correction (adjusted p = 0.231), whereas global RNFL thickness showed no significant difference. No significant differences were observed between groups in VD-DCP, FAZ area, choriocapillaris flow, optic disc vascular parameters, foveal thickness, or CCT (all p > 0.05). CONCLUSION: Patients with AA demonstrate reduced VD-SCP except in the foveal region, without evidence of widespread retinal or choroidal vascular impairment. These findings suggest that AA may be associated with early subclinical alterations in retinal microcirculation. OCTA may serve as a useful non-invasive tool for detecting subtle ocular microvascular changes in patients with AA. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature B.V. FAU - Erdem Uysal, Merve AU - Erdem Uysal M AD - Ophthalmology Clinic, Toros State Hospital, Mersin, Turkey. merveerdemuysal@gmail.com. FAU - Kurtul, Bengi Ece AU - Kurtul BE AD - Department of Ophthalmology, Faculty of Medicine, Hatay Mustafa Kemal University, Hatay, Turkey. FAU - Akbay, Selen Taner AU - Akbay ST AD - Department of Dermatology, Faculty of Medicine, Hatay Mustafa Kemal University, Hatay, Turkey. FAU - Celik, Ebru AU - Celik E AD - Private Dermatology Clinic, Siirt, Turkey. LA - eng PT - Journal Article DEP - 20261029 PL - Netherlands TA - Int Ophthalmol JT - International ophthalmology JID - 7904294 SB - IM MH - Humans MH - Female MH - *Tomography, Optical Coherence/methods MH - Male MH - Prospective Studies MH - *Retinal Vessels/pathology/diagnostic imaging MH - Adult MH - *Fluorescein Angiography/methods MH - *Alopecia Areata/diagnosis/physiopathology/complications MH - Young Adult MH - *Microvascular Density MH - Fundus Oculi MH - Microvessels/pathology MH - Adolescent MH - Retinal Ganglion Cells/pathology MH - Optic Disk/blood supply MH - Nerve Fibers/pathology MH - *Retinal Diseases/etiology/diagnosis MH - Capillaries/pathology OTO - NOTNLM OT - Alopecia areata OT - Optical coherence tomography angiography OT - Retinal microvasculature OT - Superficial capillary plexus OT - Vessel density COIS- Declarations. Competing interests: The authors have not disclosed any competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:21 PHST- 2026/05/26 00:00 [received] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:21 [entrez] AID - 10.1007/s10792-026-04327-w [pii] AID - 10.1007/s10792-026-04327-w [doi] PST - epublish SO - Int Ophthalmol. 2026 Oct 29;46(1):446. doi: 10.1007/s10792-026-04327-w. PMID- 42955432 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1438-7948 (Electronic) IS - 1438-793X (Linking) VI - 26 IP - 1 DP - 2026 Oct 29 TI - Dissecting cell-type-specific genetic associations between colorectal cancer liability and thrombotic risk in peripheral blood immune cells. LID - 309 [pii] LID - 10.1007/s10142-026-02080-y [doi] AB - Venous thromboembolism (VTE) is a major complication of colorectal cancer (CRC), but whether CRC-associated thrombotic susceptibility includes cell-specific inherited regulatory components in peripheral blood remains unclear. To investigate associations between CRC genetic liability and VTE-related outcomes within peripheral blood mononuclear cell (PBMC) regulatory contexts. We integrated CRC GWAS, FinnGen VTE-related outcomes, and TenK10K PBMC single-cell eQTL data across 28 immune cell types using cell-stratified Mendelian randomization (csMR). Prioritized signals were further examined in single-cell RNA-seq datasets and bulk transcriptomes. Conventional MR showed no clear evidence that CRC liability increased VTE, DVT, or PE risk, and LD Score regression detected no significant genome-wide genetic correlation. In contrast, csMR identified nominal, cell-context-dependent associations. B intermediate cells were prioritized across VTE (OR, 1.16; 95% CI, 1.03-1.32), DVT (OR, 1.30; 95% CI, 1.08-1.57), and PE (OR, 1.26; 95% CI, 1.07-1.49), while CD14 + monocytes and CD4 + central memory T cells received additional support from downstream single-cell analyses. Gene-level analyses prioritized LAMA5, LIMA1, and TRIM28 in these respective cell contexts. In bulk blood, RPS21 showed consistent disease-associated expression patterns and discriminated VTE patients from healthy controls in an external dataset (AUC, 0.865; 95% CI, 0.800-0.923). These findings suggest that genetic overlap between CRC liability and thrombotic phenotypes may be localized to selected PBMC regulatory contexts rather than broadly detectable genome-wide. Among the prioritized signals, B intermediate cells showed the most consistent csMR associations across VTE, DVT, and PE. The cell-gene pairs, together with RPS21, warrant independent genetic, functional, and prospective validation as candidates for studying peripheral immune regulation and blood-based markers of CRC-associated thrombosis. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Ying, Jiacheng AU - Ying J AD - Department of gastrointestinal surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China. FAU - Ling, Tianlong AU - Ling T AD - Department of gastrointestinal surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China. FAU - Fang, Zhengyuan AU - Fang Z AD - Department of gastrointestinal surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China. FAU - Zhang, Cheng AU - Zhang C AD - Department of gastrointestinal surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China. FAU - Bao, Yujie AU - Bao Y AD - Department of Infectious Diseases, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China. yujie.bao@shsmu.edu.cn. FAU - Tang, Jiayin AU - Tang J AD - Department of gastrointestinal surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200127, China. jiayintang@shsmu.edu.cn. LA - eng GR - 82273279/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261029 PL - Germany TA - Funct Integr Genomics JT - Functional & integrative genomics JID - 100939343 SB - IM MH - Humans MH - *Colorectal Neoplasms/genetics/complications/pathology/blood/immunology MH - Genome-Wide Association Study MH - Genetic Predisposition to Disease MH - *Leukocytes, Mononuclear/metabolism/immunology MH - *Venous Thromboembolism/genetics MH - Quantitative Trait Loci MH - *Thrombosis/genetics MH - Polymorphism, Single Nucleotide OTO - NOTNLM OT - Cell-stratified Mendelian Randomization OT - Colorectal Cancer OT - Genetic predisposition OT - Peripheral blood mononuclear cell OT - Venous Thromboembolism COIS- Declarations. Clinical trial number: Not applicable. Consent: The authors have nothing to report. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:20 PHST- 2026/07/29 00:00 [received] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/09/26 00:00 [revised] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:20 [entrez] AID - 10.1007/s10142-026-02080-y [pii] AID - 10.1007/s10142-026-02080-y [doi] PST - epublish SO - Funct Integr Genomics. 2026 Oct 29;26(1):309. doi: 10.1007/s10142-026-02080-y. PMID- 42955431 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1573-7217 (Electronic) IS - 0167-6806 (Linking) VI - 220 IP - 1 DP - 2026 Oct 28 TI - Real-world efficacy and safety of trastuzumab emtansine biosimilar (UJVIRA((R))) in early and metastatic HER2-positive breast cancer: a multicenter pooled retrospective analysis from India. LID - 20 [pii] LID - 10.1007/s10549-026-08114-0 [doi] AB - PURPOSE: Trastuzumab-emtansine (T-DM1), an antibody drug conjugate, is approved in the adjuvant (residual disease post-neoadjuvant chemotherapy) and metastatic settings for HER2-positive breast cancer. Real-world evidence on T-DM1 biosimilar from India remains sparse. METHODS: During October 2021-July 2025, 101 cases treated with T-DM1 biosimilar - UJVIRA ((R)), across seven oncology centers in India were retrospectively analyzed. The adjuvant cohort (n = 19), had patients with residual disease after neoadjuvant therapy and surgery, and the metastatic cohort (n = 82), comprised patients progressing on trastuzumab-chemo regimens. Primary endpoints were invasive Disease-Free Survival (iDFS) and Progression Free Survival (PFS) in the adjuvant and metastatic cohort respectively. Overall Survival (OS) and safety were secondary endpoints. RESULTS: In the adjuvant cohort (median follow-up 19 months), median iDFS was not reached (mean 14.6 months), with 2 events (10.5%). In the metastatic cohort (median follow-up 8 months), median PFS was 7.5 months (mean 8.3 months; 61 events, 74.4%). PFS was longer when T-DM1 was administered earlier: 28 months in first-line, 9 months in second-line, and 8 months in third- and fourth-line (p = 0.06). Median OS in the metastatic cohort was 15.5 months and 36 months with 1st line T-DM1. In 14.6% of metastatic patients, grade 3 thrombocytopenia occurred; there were no grade 3/4 events in the adjuvant cohort. CONCLUSION: UJVIRA((R)) demonstrated real-world clinical effectiveness and an acceptable safety profile in both adjuvant and metastatic HER2-positive breast cancer, with outcomes similar to landmark clinical trials supporting its role as a cost-effective biosimilar in India. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Mahajan, Ayesha AU - Mahajan A AUID- ORCID: 0009-0006-2245-382X AD - Department of Medical Oncology, Institute of Medical Sciences and Sum Hospital, Siksha 'O' Anusandhan Deemed to be University, Bhubaneswar, Odisha, 751003, India. FAU - Panda, Soumya Surath AU - Panda SS AUID- ORCID: 0000-0003-2190-1927 AD - Department of Medical Oncology, Institute of Medical Sciences and Sum Hospital, Siksha 'O' Anusandhan Deemed to be University, Bhubaneswar, Odisha, 751003, India. soumyasurathpanda@soa.ac.in. FAU - Moharana, Lalatendu AU - Moharana L AUID- ORCID: 0000-0002-5426-0356 AD - Department of Medical Oncology, Institute of Medical Sciences and Sum Hospital, Siksha 'O' Anusandhan Deemed to be University, Bhubaneswar, Odisha, 751003, India. FAU - Chandrakanth, M V AU - Chandrakanth MV AD - Department of Medical Oncology, Narayana Superspeciality Hospital, Shibpur, Howrah, West Bengal, 711103, India. FAU - Agarwala, Vivek AU - Agarwala V AD - Department of Medical Oncology, Narayana Superspeciality Hospital, Shibpur, Howrah, West Bengal, 711103, India. FAU - Manna, Rupam AU - Manna R AD - Department of Medical Oncology, Narayana Superspeciality Hospital, Shibpur, Howrah, West Bengal, 711103, India. FAU - Saju, S V AU - Saju SV AUID- ORCID: 0000-0003-0268-7937 AD - Department of Medical Oncology, Meenakshi Mission Medical College and Research Center, Madurai, Tamil nadu, 625 107, India. FAU - Raju, Susan Honey AU - Raju SH AUID- ORCID: 0000-0002-4599-055X AD - Department of Medical Oncology, Meenakshi Mission Medical College and Research Center, Madurai, Tamil nadu, 625 107, India. FAU - Ganesan, Prasanth AU - Ganesan P AD - Department of Medical Oncology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, 605 006, India. FAU - Shankar, Gomathi AU - Shankar G AD - Department of Surgery, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, 605 006, India. FAU - Phillip, Ashwin Oommen AU - Phillip AO AUID- ORCID: 0000-0002-1385-0392 AD - Department of Medical Oncology, Amala Institute of Medical Sciences, Thrissur, Kerala, 680 555, India. FAU - Cyriac, Sunu Lazar AU - Cyriac SL AD - Department of Medical Oncology, Amala Institute of Medical Sciences, Thrissur, Kerala, 680 555, India. FAU - Jose, Anil AU - Jose A AD - Department of Medical Oncology, Amala Institute of Medical Sciences, Thrissur, Kerala, 680 555, India. FAU - Unnikrishnan, P AU - Unnikrishnan P AD - Department of Medical Oncology, Amala Institute of Medical Sciences, Thrissur, Kerala, 680 555, India. FAU - Bhatia, Kanu Priya AU - Bhatia KP AUID- ORCID: 0009-0006-9732-3560 AD - Department of Medical Oncology, Mohandai Oswal Hospital, Ludhiana, Punjab, 141 009, India. FAU - Sehrawat, Amit AU - Sehrawat A AUID- ORCID: 0000-0001-7100-8999 AD - Department of Medical Oncology Haematology, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, 249 203, India. FAU - Sundriyal, Deepak AU - Sundriyal D AUID- ORCID: 0000-0002-6378-7694 AD - Department of Medical Oncology Haematology, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, 249 203, India. FAU - Dhanai, Himani AU - Dhanai H AD - Department of Medical Oncology Haematology, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, 249 203, India. FAU - Samantaray, Lipsita AU - Samantaray L AUID- ORCID: 0009-0002-7909-6844 AD - Department of Medical Oncology, Institute of Medical Sciences and Sum Hospital, Siksha 'O' Anusandhan Deemed to be University, Bhubaneswar, Odisha, 751003, India. FAU - Mohanty, Swati Sucharita AU - Mohanty SS AUID- ORCID: 0000-0003-1497-6293 AD - Department of Medical Oncology, Institute of Medical Sciences and Sum Hospital, Siksha 'O' Anusandhan Deemed to be University, Bhubaneswar, Odisha, 751003, India. FAU - Dubashi, Biswajit AU - Dubashi B AD - Department of Medical Oncology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, 605 006, India. LA - eng PT - Journal Article PT - Multicenter Study DEP - 20261028 PL - Netherlands TA - Breast Cancer Res Treat JT - Breast cancer research and treatment JID - 8111104 RN - EC 2.7.10.1 (Erb-b2 Receptor Tyrosine Kinases) RN - SE2KH7T06F (Ado-Trastuzumab Emtansine) RN - 0 (Biosimilar Pharmaceuticals) RN - EC 2.7.10.1 (ERBB2 protein, human) RN - P188ANX8CK (Trastuzumab) SB - IM MH - Humans MH - Female MH - *Breast Neoplasms/drug therapy/pathology/mortality/metabolism MH - India/epidemiology MH - *Erb-b2 Receptor Tyrosine Kinases/metabolism MH - *Ado-Trastuzumab Emtansine/therapeutic use/adverse effects/administration & dosage MH - Retrospective Studies MH - Middle Aged MH - *Biosimilar Pharmaceuticals/therapeutic use/adverse effects/administration & dosage MH - Aged MH - Adult MH - Trastuzumab MH - Neoplasm Metastasis MH - Treatment Outcome OTO - NOTNLM OT - HER2-positive breast cancer OT - Real-world evidence OT - T-DM1 biosimilar OT - Trastuzumab emtansine OT - UJVIRA(R) COIS- Declarations. Ethical approval and consent to participate: This study was approved by the institutional review board (IMS.SH/SOA/2021/097; dated 7th July 2021), and patient consent was obtained. All procedures performed in our study involving human participation were in accordance with the ethical standards of the institutional and/or national research committee and in compliance with the 1964 Declaration of Helsinki and its later amendments. Clinical Trials Registry-India (CTRI)Number: CTRI/2022/01/039233 Dated 07/01/2022 Consent for publication: Informed consent was obtained from all patients for both participation and the publication of their details. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:20 PHST- 2026/07/10 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:20 [entrez] AID - 10.1007/s10549-026-08114-0 [pii] AID - 10.1007/s10549-026-08114-0 [doi] PST - epublish SO - Breast Cancer Res Treat. 2026 Oct 28;220(1):20. doi: 10.1007/s10549-026-08114-0. PMID- 42955415 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1438-7948 (Electronic) IS - 1438-793X (Linking) VI - 26 IP - 1 DP - 2026 Oct 29 TI - Deep learning-based multiomics integration identifies AI-defined tissue regions associated with cancer-associated fibroblast enrichment and chemoresistance in muscle-invasive bladder cancer. LID - 308 [pii] LID - 10.1007/s10142-026-02086-6 [doi] AB - Muscle-invasive bladder cancer (MIBC) frequently develops resistance to platinum-based chemotherapy, and tools for characterizing treatment-associated tumor microenvironmental features remain limited. We developed an artificial intelligence (AI)-based framework integrating pathological imaging, multiomics data, single-cell RNA sequencing, and ex vivo drug-sensitivity testing. Three convolutional neural network models trained using hematoxylin-and-eosin-stained whole-slide images from The Cancer Genome Atlas (TCGA) classified tumor, necrosis, and lymphocyte-enriched regions and were evaluated in an independent tissue microarray cohort. Patches negative for all three classifiers were designated AI-defined "Other" regions; this residual category was not trained as a cancer-associated fibroblast (CAF) classifier. Higher Other% was associated with shorter overall survival in univariable analyses, but the association was not significant after multivariable adjustment in TCGA. Multiomics and tissue-origin analyses linked Other% to stromal content. Scissor analysis of eight single-cell RNA-sequencing datasets identified Other-associated Scissor+ cells across epithelial and fibroblast compartments, while ACTA2 expression and extracellular matrix-remodeling pathways were enriched. In 48 MIBC specimens assessed by the hydrogel-embedded histoculture drug sensitivity test, Other% was higher in the gemcitabine-cisplatin-resistant group. Multiplex immunofluorescence further showed enrichment of alpha-smooth muscle actin signal in AI-defined Other regions. Erdafitinib showed ex vivo activity in a subset of specimens, but FGFR alteration status was unavailable and clinical efficacy was not evaluated. These findings support an association between AI-defined Other regions, CAF-related stromal features, and chemoresistance, while prospective validation is required before clinical use. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Hu, Chao AU - Hu C AD - Department of Urology, The Second Affiliated Hospital of Nanchang University, Nanchang University, No. 1 Minde Road, Nanchang, 330000, China. FAU - Wang, Fan AU - Wang F AD - Department of Artificial Intelligence and Informatization, The Second Affiliated Hospital of Nanchang University, Nanchang University, Nanchang, China. FAU - Zhang, Qing AU - Zhang Q AD - Department of Emergency, Jiangxi Maternal and Child Health Hospital, Nanchang Medical College, Nanchang, China. FAU - Xu, Hui AU - Xu H AD - School of Public Health, Jiangxi Provincial Key Laboratory of Disease Prevention and Public Health, Nanchang University, Nanchang University, No. 999 Xuefu Avenue, Nanchang, 330000, China. FAU - Huang, Jing AU - Huang J AD - School of Public Health, Jiangxi Provincial Key Laboratory of Disease Prevention and Public Health, Nanchang University, Nanchang University, No. 999 Xuefu Avenue, Nanchang, 330000, China. FAU - Xiong, Xiujuan AU - Xiong X AD - School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang, China. FAU - Huang, Yuxin AU - Huang Y AD - Department of Pathology, The Second Affiliated Hospital of Nanchang University, Nanchang University, Nanchang, China. FAU - Lou, Weiming AU - Lou W AD - Department of Educational Administration, The Second Affiliated Hospital of Nanchang University, Nanchang University, Nanchang, China. FAU - He, Yuanqiao AU - He Y AD - Department of Jiangxi Province Key Laboratory of Laboratory Animal, Nanchang University, Nanchang, China. FAU - Deng, Libin AU - Deng L AD - School of Public Health, Jiangxi Provincial Key Laboratory of Disease Prevention and Public Health, Nanchang University, Nanchang University, No. 999 Xuefu Avenue, Nanchang, 330000, China. lbdeng@ncu.edu.cn. FAU - Lu, Xiongbing AU - Lu X AD - Department of Urology, The Second Affiliated Hospital of Nanchang University, Nanchang University, No. 1 Minde Road, Nanchang, 330000, China. 995374160@qq.com. LA - eng GR - No. 202610444/the Science and Technology Program of Jiangxi Provincial Health Commission/ GR - No. 202510543/the Science and Technology Program of Jiangxi Provincial Health Commission/ GR - No. 82060465/the National Natural Science Foundation of China/ GR - No. 20212ACB206023/the Natural Science Foundation of Jiangxi Province/ PT - Journal Article DEP - 20261029 PL - Germany TA - Funct Integr Genomics JT - Functional & integrative genomics JID - 100939343 RN - 0W860991D6 (Deoxycytidine) SB - IM MH - Humans MH - *Deep Learning MH - *Urinary Bladder Neoplasms/pathology/genetics/drug therapy/metabolism MH - *Cancer-Associated Fibroblasts/metabolism/pathology/drug effects MH - *Drug Resistance, Neoplasm/genetics MH - Tumor Microenvironment MH - Multiomics MH - Deoxycytidine/analogs & derivatives/pharmacology OTO - NOTNLM OT - Cancer-associated fibroblasts OT - Chemoresistance OT - Muscle-invasive bladder cancer OT - Pathological artificial intelligence OT - Tumor microenvironment COIS- Declarations. Ethics statement: The TMA HBlaU079Su01 used in this study was approved by the Ethics Committee of Shanghai Outdo Biotech Company (Approval number: YBM-05-01) on October 11, 2019. Consent to participate: This retrospective study was conducted using archived tissue samples and de-identified clinical data. The requirement for written informed consent was waived by the Ethics Committee of the Second Affiliated Hospital of Nanchang University (approval date: September 13, 2024). All patient data were anonymized prior to analysis, and the study was performed in accordance with the Declaration of Helsinki. Clinical trial number: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:19 PHST- 2026/08/19 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:19 [entrez] AID - 10.1007/s10142-026-02086-6 [pii] AID - 10.1007/s10142-026-02086-6 [doi] PST - epublish SO - Funct Integr Genomics. 2026 Oct 29;26(1):308. doi: 10.1007/s10142-026-02086-6. PMID- 42955413 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1438-7948 (Electronic) IS - 1438-793X (Linking) VI - 26 IP - 1 DP - 2026 Oct 29 TI - BILBO: a self-hosted platform for large-scale RNA-Seq analysis with a functional annotation clustering framework. LID - 306 [pii] LID - 10.1007/s10142-026-02091-9 [doi] AB - RNA sequencing (RNA-Seq) has become a central approach for transcriptomic studies, but its practical application still requires the coordination of multiple computational steps and often produces large gene expression outputs whose integrative biological interpretation remains challenging. Here, we present BILBO (Bioinformatics Integration for Large-scale Biological Operations), a self-hosted and fully containerized platform designed to support reproducible, end-to-end RNA-Seq analyses, from data acquisition to integrative functional interpretation. BILBO consolidates widely adopted open-source tools into standardized workflows, enabling automated retrieval of public or private RNA-Seq data, quality control, alignment, quantification, normalization, differential expression analysis, and generation of publication-ready outputs within a unified environment. Beyond workflow integration, BILBO introduces a novel functional annotation clustering framework that organizes genes using Gene Ontology (GO)-based semantic similarity. The approach separates genes by regulatory direction and ontology, computes Wang semantic similarity among GO annotations, applies complete-linkage hierarchical clustering, refines assignments through silhouette-based pruning, and selects representative genes as semantic medoids. To further enhance biological interpretability, BILBO integrates a Retrieval-Augmented Generation strategy that links representative genes and functional clusters to curated scientific literature through hybrid retrieval and DOI-linked evidence chunks. In a controlled SEQC/MAQC-III benchmark against nf-core/rnaseq, BILBO preserved differential-expression effect sizes (cross-workflow Spearman >/= 0.994) and the expected behavior of the reference mixtures, whereas BILBO required 68% less peak temporary disk space but 25% more wall time. In the SEQC dataset, the resulting clusters showed strong coherence with independent MSigDB pathways, comparable to a GOSemSim-based reference workflow, and remained stable under moderate parameter changes. Together, these features position BILBO as an integrative and scalable solution that bridges the gap between large-scale RNA-Seq data processing and higher-level functional insight, promoting reproducibility, interpretability, and accessibility in transcriptomic research. CI - (c) 2026. The Author(s). FAU - da Silva, Vitor Luciano Costa AU - da Silva VLC AUID- ORCID: 0009-0002-9304-5699 AD - Institute of Natural Sciences, Department of Biology, Sector of Plant Physiology, Laboratory of Plant Molecular Physiology, Federal University of Lavras, Lavras, Minas Gerais, Brazil. FAU - Alvarenga, Joao Vitor Reis AU - Alvarenga JVR AUID- ORCID: 0009-0004-5636-2526 AD - Institute of Natural Sciences, Department of Biology, Sector of Plant Physiology, Laboratory of Plant Molecular Physiology, Federal University of Lavras, Lavras, Minas Gerais, Brazil. FAU - Linhares-Neto, Manoel Viana AU - Linhares-Neto MV AUID- ORCID: 0000-0002-8244-7177 AD - Institute of Natural Sciences, Department of Biology, Sector of Plant Physiology, Laboratory of Plant Molecular Physiology, Federal University of Lavras, Lavras, Minas Gerais, Brazil. FAU - Noman, Muhammad AU - Noman M AUID- ORCID: 0000-0002-5580-671X AD - Institute of Natural Sciences, Department of Biology, Sector of Plant Physiology, Laboratory of Plant Molecular Physiology, Federal University of Lavras, Lavras, Minas Gerais, Brazil. FAU - Chalfun-Junior, Antonio AU - Chalfun-Junior A AUID- ORCID: 0000-0002-1794-9429 AD - Institute of Natural Sciences, Department of Biology, Sector of Plant Physiology, Laboratory of Plant Molecular Physiology, Federal University of Lavras, Lavras, Minas Gerais, Brazil. chalfunjunior@ufla.br. LA - eng PT - Journal Article DEP - 20261029 PL - Germany TA - Funct Integr Genomics JT - Functional & integrative genomics JID - 100939343 SB - IM MH - *Software MH - *Molecular Sequence Annotation MH - Clustering Algorithms MH - *RNA-Seq/methods MH - *Computational Biology/methods MH - Cluster Analysis MH - *Sequence Analysis, RNA/methods MH - Gene Ontology MH - Gene Expression Profiling/methods OTO - NOTNLM OT - Bioinformatics OT - DEG OT - Large language models OT - Transcriptomics COIS- Declarations. Ethics approval: Not applicable. Consent to participate: Not Applicable. Consent for publication: All authors consent to publish. Clinical trial number: Not applicable. Conflicts of interest/Competing interests: The authors declare no conflict of interest. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:19 PHST- 2026/04/14 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/08/17 00:00 [revised] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:19 [entrez] AID - 10.1007/s10142-026-02091-9 [pii] AID - 10.1007/s10142-026-02091-9 [doi] PST - epublish SO - Funct Integr Genomics. 2026 Oct 29;26(1):306. doi: 10.1007/s10142-026-02091-9. PMID- 42955405 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1432-1076 (Electronic) IS - 0340-6199 (Linking) VI - 185 IP - 11 DP - 2026 Oct 29 TI - Proportionate antimicrobial use in pediatric end-of-life care: a narrative review and practical framework. LID - 906 [pii] LID - 10.1007/s00431-026-07570-6 [doi] AB - Antimicrobial prescribing in pediatric palliative care is frequent, emotionally charged, and clinically complex. In children approaching the end of life, antimicrobial therapy may offer symptom relief in selected circumstances, but it may also add procedural burden, adverse effects, and a risk of prolonging the dying process without improving comfort. The objective is to synthesize the limited pediatric evidence, distinguish it from adult palliative-care evidence and general stewardship guidance, and propose a practical operational framework for decision-making in pediatric end-of-life care. This study is a narrative review of the infectious-disease and palliative-care literature, with a targeted PubMed search and hand-searching of relevant reference lists and guidance documents. The review focused on symptom-oriented benefits, treatment-related burdens, the ethical principles of beneficence, distributive justice, and proportionality, antimicrobial stewardship, and the role of shared decision-making and clinical documentation in pediatric practice. The literature search was last updated in March 2026. For each major clinical proposition, we distinguished direct pediatric end-of-life evidence from adult palliative-care evidence, general pediatric infectious-disease or stewardship guidance, and the authors' reasoned recommendations. Available evidence suggests that antimicrobials may be proportionate when symptomatic benefit is plausible, perceptible, and aligned with the goals of care. However, benefits are inconsistent, pediatric evidence remains limited, and antimicrobial therapy is often prescribed empirically even when its palliative value is uncertain. Direct pediatric evidence is sparse and largely observational or descriptive. Across studies and guidelines, recurrent principles emerge: avoid automatic prescribing, define the goal of treatment, prefer the least burdensome option, document the rationale, and reassess within 48-72 h. CONCLUSION: In pediatric end-of-life care, the key question is not simply whether an infection is present, but whether treating it is likely to meaningfully and proportionately improve the child's comfort. A structured framework that integrates ethics, antimicrobial stewardship, shared decision-making, and clear documentation may help clinicians move from reflexive prescribing to deliberate, proportionate decision-making in pediatric palliative care. WHAT IS KNOWN: * Direct pediatric evidence on antimicrobial use at the end of life is sparse,largely descriptive, and provides limited information on patient-centered symptomatic benefit. WHAT IS NEW: * This narrative review explicitly separates pediatric evidence from adult extrapolation and general stewardship guidance and proposes an authorderived practical framework linking comfort goals, proportionality, child participation, early reassessment, and documentation. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Bucci, Luca Maria AU - Bucci LM AD - Division of Infectious Diseases, Department of Health Sciences (DISSAL), University of Genova, Genoa, Italy. AD - IRCCS-AOM Azienda Ospedaliera Metropolitana - Policlinico San Martino, Genoa, Italy. FAU - Bonsignore, Alessandro AU - Bonsignore A AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Brisca, Giacomo AU - Brisca G AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Castellani, Carlo AU - Castellani C AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Dallorso, Sandro AU - Dallorso S AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Dufour, Carlo AU - Dufour C AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Faraci, Maura AU - Faraci M AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Manfredini, Luca AU - Manfredini L AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Mariani, Marcello AU - Mariani M AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Mesini, Alessio AU - Mesini A AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Milanaccio, Claudia AU - Milanaccio C AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Monti, Bianca AU - Monti B AD - School of Medicine and Surgery, University of Milano-Bicocca, Milan, Italy. FAU - Moscatelli, Andrea AU - Moscatelli A AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Pirlo, Daniela AU - Pirlo D AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Ramenghi, Luca AU - Ramenghi L AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Ricci, Erica AU - Ricci E AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Saffioti, Carolina AU - Saffioti C AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Santaniello, Maria AU - Santaniello M AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Spiazzi, Raffaele AU - Spiazzi R AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Spiga, Giuseppe AU - Spiga G AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Spreafico, Filippo AU - Spreafico F AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Toto, Martina AU - Toto M AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. FAU - Castagnola, Elio AU - Castagnola E AUID- ORCID: 0000-0002-5336-8429 AD - IRCCS Istituto Giannina Gaslini, Genoa, Italy. eliocastagnola@gaslini.org. LA - eng GR - Ricerca corrente/Ministero della Salute/ PT - Journal Article PT - Review DEP - 20261029 PL - Germany TA - Eur J Pediatr JT - European journal of pediatrics JID - 7603873 RN - 0 (Anti-Infective Agents) SB - IM MH - Humans MH - *Terminal Care/methods MH - Child MH - *Antimicrobial Stewardship MH - *Palliative Care/methods MH - *Anti-Infective Agents/therapeutic use MH - Pediatrics OTO - NOTNLM OT - Anti-infective therapy OT - Narrative review OT - Pediatric end-of-life care OT - Practical framework COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:19 PHST- 2026/05/04 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/20 00:00 [revised] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:19 [entrez] AID - 10.1007/s00431-026-07570-6 [pii] AID - 10.1007/s00431-026-07570-6 [doi] PST - epublish SO - Eur J Pediatr. 2026 Oct 29;185(11):906. doi: 10.1007/s00431-026-07570-6. PMID- 42955403 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1436-5073 (Electronic) IS - 0026-3672 (Linking) VI - 193 IP - 11 DP - 2026 Oct 28 TI - MWCNT-MOF-199-Nafion hybrid platform for low-potential amperometric nitrite sensing. LID - 767 [pii] LID - 10.1007/s00604-026-08479-5 [doi] AB - A simple electrochemical sensing platform based on a hybrid composed of multi-walled carbon nanotubes (MWCNTs), MOF-199, and Nafion is reported for the amperometric determination of nitrite at a low potential (- 0.200 V). Most electrochemical nitrite sensors rely on its oxidation at relatively high positive potentials, where the oxidation of coexisting electroactive species may compromise selectivity. The sensing strategy exploits the Cu(II)/Cu(I) redox couple of MOF-199 to mediate the catalytic reduction of nitrite, while MWCNTs provide an efficient conductive network that facilitates electron transfer. Nafion promotes homogeneous dispersion of the hybrid components, improves interfacial contact, and enhances the structural stability of the sensing layer, resulting in a robust architecture. Under the optimized experimental conditions, the proposed sensor exhibited a sensitivity of (2.60 +/- 0.03) microA ppm(-)(1), a detection limit of 21 ppb (0.45 microM), a wide linear range, good reproducibility intra-hybrid (RSD = 7.7%) and inter-hybrid (RSD = 2.6%), excellent long-term stability, and negligible interference from common inorganic ions and electroactive compounds. The practical applicability of the sensing platform was successfully demonstrated through nitrite determination in different water samples. The proposed sensing platform combines straightforward fabrication, good analytical performance, and practical applicability. More importantly, it demonstrates that the rational integration of conductive carbon nanostructures with redox-active MOFs provides an effective strategy for developing simple and robust electrochemical sensors operating through catalytic nitrite. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature. FAU - Aghemo, Facundo AU - Aghemo F AUID- ORCID: 0000-0002-8229-182X AD - Centro de Investigacion y Transferencia en Ingenieria Quimica Ambiental (CIQA), CONICET, Departamento de Ingenieria Quimica, Facultad Regional Cordoba, Universidad Tecnologica Nacional, Maestro Lopez esq. Cruz Roja Argentina, X5016ZAA, Cordoba, Argentina. AD - Instituto de Investigaciones en Fisico-Quimica de Cordoba (INFIQC), CONICET-UNC, Departamento de Fisicoquimica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Ciudad Universitaria, X5000HUA, Cordoba, Argentina. FAU - Vaschetti, Virginia AU - Vaschetti V AUID- ORCID: 0000-0001-7708-049X AD - Centro de Investigacion y Transferencia en Ingenieria Quimica Ambiental (CIQA), CONICET, Departamento de Ingenieria Quimica, Facultad Regional Cordoba, Universidad Tecnologica Nacional, Maestro Lopez esq. Cruz Roja Argentina, X5016ZAA, Cordoba, Argentina. FAU - Mujica, Michael Lopez AU - Mujica ML AUID- ORCID: 0000-0002-8585-3920 AD - Centro de Investigacion y Transferencia en Ingenieria Quimica Ambiental (CIQA), CONICET, Departamento de Ingenieria Quimica, Facultad Regional Cordoba, Universidad Tecnologica Nacional, Maestro Lopez esq. Cruz Roja Argentina, X5016ZAA, Cordoba, Argentina. FAU - Otal, Eugenio AU - Otal E AUID- ORCID: 0000-0003-0801-854X AD - Institute for Aqua Regeneration, Shinshu University, 4-17-1 Wakasato, Nagano, 380-8553, Japan. FAU - Teshima, Katsuya AU - Teshima K AUID- ORCID: 0000-0002-5784-5157 AD - Institute for Aqua Regeneration, Shinshu University, 4-17-1 Wakasato, Nagano, 380-8553, Japan. FAU - Rivas, Gustavo AU - Rivas G AUID- ORCID: 0000-0001-7411-6821 AD - Instituto de Investigaciones en Fisico-Quimica de Cordoba (INFIQC), CONICET-UNC, Departamento de Fisicoquimica, Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Ciudad Universitaria, X5000HUA, Cordoba, Argentina. gustavo.rivas@unc.edu.ar. FAU - Dalmasso, Pablo AU - Dalmasso P AUID- ORCID: 0000-0003-1314-7135 AD - Centro de Investigacion y Transferencia en Ingenieria Quimica Ambiental (CIQA), CONICET, Departamento de Ingenieria Quimica, Facultad Regional Cordoba, Universidad Tecnologica Nacional, Maestro Lopez esq. Cruz Roja Argentina, X5016ZAA, Cordoba, Argentina. pdalmasso@frc.utn.edu.ar. LA - eng GR - PID COMSEC451/Universidad Tecnologica Nacional/ GR - PID MSECCO0010216TC/Universidad Tecnologica Nacional/ GR - SECyT-UNC PIDTA 2023/Universidad Nacional de Cordoba/ GR - PIP 2022-11220210100957CO/Consejo Nacional de Investigaciones Cientificas y Tecnicas/ GR - PICT 2019-0411/ANPCyT-FONCyT/ PT - Journal Article DEP - 20261028 PL - Austria TA - Mikrochim Acta JT - Mikrochimica acta JID - 7808782 SB - IM OTO - NOTNLM OT - Carbon nanotubes OT - Electrochemical sensor OT - Hybrid material OT - MOF-199 OT - Nafion OT - Nitrite COIS- Declarations. Competing interests: The authors declare no competing interests. AI disclosure statement: ChatGPT was used solely to improve the grammar and language of the manuscript. No AI tools were used to generate, analyze, or interpret the scientific data, prepare the figures or graphical abstract, or formulate scientific conclusions. All scientific content was conceived, verified, and approved by the authors. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:18 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:18 [entrez] AID - 10.1007/s00604-026-08479-5 [pii] AID - 10.1007/s00604-026-08479-5 [doi] PST - epublish SO - Mikrochim Acta. 2026 Oct 28;193(11):767. doi: 10.1007/s00604-026-08479-5. PMID- 42955402 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1432-1076 (Electronic) IS - 0340-6199 (Linking) VI - 185 IP - 11 DP - 2026 Oct 28 TI - External validation of the MDR score for predicting multidrug-resistant device-associated infections in pediatric intensive care units (PEPE-MDR score): a nationwide registry-based study. LID - 900 [pii] LID - 10.1007/s00431-026-07504-2 [doi] AB - Multidrug-resistant (MDR) organisms are an increasing cause of device-associated healthcare-associated infections (DA-HAIs) in pediatric intensive care units (PICUs), complicating empirical antibiotic selection. The aim of the study was to externally validate the MDR-score (PEPE-MDR score), a tool developed to estimate the likelihood of a DA-HAI being caused by MDR. A multicenter retrospective cohort study was conducted using surveillance data from the pediatric ENVIN-HELICS registry (2014-2024). Pediatric patients with microbiologically confirmed DA-HAIs were included. The PEPE-MDR score incorporates prior MDR colonization, comorbidity, and PICU length of stay > 9 days. Discrimination was assessed using area under the receiver operating characteristic curve (AUC-ROC), sensitivity, specificity, and positive (PPV) and negative predictive values (NPV). Calibration was evaluated with the observed-to-expected (O:E) ratio and the calibration slope. Among 21,999 episodes registered, 406 DA-HAIs were included. Of these, 93 (22.9%) were caused by MDR organisms. Median (IQR) MDR scores were higher in MDR infections than non-MDR infections (p < 0.001), with a Hodges-Lehmann location shift (95% CI) of 3 points (2-4). The MDR score yielded an AUC-ROC of 0.69 (95% CI 0.62-0.75). Using the high-risk category as the threshold, sensitivity and specificity were 46.2% and 79.9% (PPV 40.6%; NPV 83.3%). Combining moderate- and high-risk categories increased sensitivity to 77.4% with specificity 47.0% (PPV 30.3%; NPV 87.5%). Observed risks were lower than predicted: O:E ratio 0.64 (95% CI 0.54-0.77), although risk increased across categories (12.5%, 22.0%, 40.6%; p < 0.001). CONCLUSION: In this multicenter external validation, the PEPE-MDR score provided moderate discrimination and preserved risk stratification for MDR DA-HAIs, contributing to empirical antibiotic decision-making in PICUs after recalibration to local prevalence. WHAT IS KNOWN: * Acknowledging the risk of a multidrug resistant microorganism involvement in infections related to invasive devices is crucial to adjust empirical antibiotherapy in the pediatric intensive care unit (PICU). * A MDR score for Predicting Multidrug-Resistant Device-Associated Infections in Pediatric Intensive Care Units (PEPE-MDR score) was developed recently, but it required external validation. WHAT IS NEW: * The PEPE-MDR score was validated in a multicenter national database. * These findings support the PEPE-MDR score's utility as a practical tool for adjusting empirical antibiotic treatment in the PICU setting. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Gonzalez-Anleo, Cristina AU - Gonzalez-Anleo C AD - Pharmacy Department, Children's Hospital Vall d'Hebron, Passeig de La Vall d'Hebron, 119-129, Barcelona, Spain. AD - Pharmacy Department, Hospital Sant Joan de Deu, University of Barcelona, Passeig Sant Joan de Deu 2, 08950, Barcelona, Spain. AD - Universitat de Barcelona (UB), c/Casanova, 143, 08036, Barcelona, Spain. FAU - Girona-Alarcon, Monica AU - Girona-Alarcon M AUID- ORCID: 0000-0002-2752-7481 AD - Pediatric Intensive Care Unit. Hospital Sant Joan de Deu, University of Barcelona, Passeig Sant Joan de Deu 2, 08950, Barcelona, Spain. monica.girona@sjd.es. AD - Immunological and Respiratory Disorders in the Pediatric Critical Patient Research Group, Institut de Recerca Sant Joan de Deu, C. de Sta. Rosa, 39, 08950, Barcelona, Spain. monica.girona@sjd.es. AD - Paediatric and Neonatal Emergency Transport System, Hospital Sant Joan de Deu, Barcelona, Spain. monica.girona@sjd.es. FAU - Fresan-Ruiz, Elena AU - Fresan-Ruiz E AD - Pediatric Intensive Care Unit. Hospital Sant Joan de Deu, University of Barcelona, Passeig Sant Joan de Deu 2, 08950, Barcelona, Spain. AD - Immunological and Respiratory Disorders in the Pediatric Critical Patient Research Group, Institut de Recerca Sant Joan de Deu, C. de Sta. Rosa, 39, 08950, Barcelona, Spain. AD - Paediatric and Neonatal Emergency Transport System, Hospital Sant Joan de Deu, Barcelona, Spain. FAU - Bobillo-Perez, Sara AU - Bobillo-Perez S AD - Pediatric Intensive Care Unit. Hospital Sant Joan de Deu, University of Barcelona, Passeig Sant Joan de Deu 2, 08950, Barcelona, Spain. AD - Immunological and Respiratory Disorders in the Pediatric Critical Patient Research Group, Institut de Recerca Sant Joan de Deu, C. de Sta. Rosa, 39, 08950, Barcelona, Spain. FAU - Guitart, Carmina AU - Guitart C AD - Pediatric Intensive Care Unit. Hospital Sant Joan de Deu, University of Barcelona, Passeig Sant Joan de Deu 2, 08950, Barcelona, Spain. AD - Immunological and Respiratory Disorders in the Pediatric Critical Patient Research Group, Institut de Recerca Sant Joan de Deu, C. de Sta. Rosa, 39, 08950, Barcelona, Spain. FAU - Garrido, Claudia AU - Garrido C AD - Institut de Recerca Sant Joan de Deu (IRSJD), C. de Sta. Rosa, 39, 08950, Barcelona, Spain. FAU - Carnaval, Thiago AU - Carnaval T AD - Institut de Recerca Sant Joan de Deu (IRSJD), C. de Sta. Rosa, 39, 08950, Barcelona, Spain. FAU - Jordan, Iolanda AU - Jordan I AD - Universitat de Barcelona (UB), c/Casanova, 143, 08036, Barcelona, Spain. AD - Pediatric Intensive Care Unit. Hospital Sant Joan de Deu, University of Barcelona, Passeig Sant Joan de Deu 2, 08950, Barcelona, Spain. AD - Immunological and Respiratory Disorders in the Pediatric Critical Patient Research Group, Institut de Recerca Sant Joan de Deu, C. de Sta. Rosa, 39, 08950, Barcelona, Spain. AD - Pediatric Infectious Diseases and Microbiome Research Group, Institut de Recerca Sant Joan de Deu, C. de Sta. Rosa, 39, 08950, Barcelona, Spain. AD - Consorcio de Investigacion Biomedica en Red de Epidemiologia y Salud (CIBERESP), Madrid, Spain. CN - Pediatric ENVIN-HELICS Study Group LA - eng PT - Journal Article PT - Multicenter Study PT - Validation Study DEP - 20261028 PL - Germany TA - Eur J Pediatr JT - European journal of pediatrics JID - 7603873 SB - IM MH - Humans MH - *Intensive Care Units, Pediatric MH - Retrospective Studies MH - *Cross Infection/microbiology/diagnosis/epidemiology MH - Registries MH - Female MH - *Drug Resistance, Multiple, Bacterial MH - Child MH - Child, Preschool MH - Male MH - Infant MH - Predictive Value of Tests MH - Sensitivity and Specificity MH - *Catheter-Related Infections/microbiology/epidemiology/diagnosis MH - ROC Curve MH - Risk Assessment/methods OTO - NOTNLM OT - Antimicrobial resistance OT - Device-associated healthcare-associated infections OT - Multidrug-resistant organisms OT - Pediatric intensive care unit OT - Risk prediction score COIS- Declarations. Competing interests: The authors declare no competing interests. FIR - Fresan, Elena IR - Fresan E FIR - Mor, Mireia IR - Mor M FIR - Garcia Salido, Alberto IR - Garcia Salido A FIR - Nieto Moro, Montserrat IR - Nieto Moro M FIR - Castro Parga, Luis Elias IR - Castro Parga LE FIR - Schuffelmann, Cristina IR - Schuffelmann C FIR - Laplaza Alonso, Maria IR - Laplaza Alonso M FIR - Pujol, Montserrat IR - Pujol M FIR - Pena, Yolanda IR - Pena Y FIR - Rubio Pascual, Paloma IR - Rubio Pascual P FIR - Belda Hofheinz, Sylvia IR - Belda Hofheinz S FIR - Ramos, Victoria IR - Ramos V FIR - Garcia Soler, Patricia IR - Garcia Soler P FIR - Sanchiz Cardenas, Sonia IR - Sanchiz Cardenas S FIR - Morteruel Arizcuren, Elvira IR - Morteruel Arizcuren E FIR - Madurga Revilla, Paula IR - Madurga Revilla P FIR - Alvarez, Ana Ortiz IR - Alvarez AO FIR - de Carlos, Juan Carlos IR - de Carlos JC FIR - Ballesta Yague, Amalia IR - Ballesta Yague A FIR - Slocker Barrio, Maria IR - Slocker Barrio M FIR - Herrera Castillo, Laura Ximena IR - Herrera Castillo LX FIR - Calvo Monge, Cristina IR - Calvo Monge C FIR - Onate, Eider IR - Onate E FIR - Arjona Villanueva, David IR - Arjona Villanueva D FIR - Gonzalez Lorenzo, Marta IR - Gonzalez Lorenzo M FIR - Oyaguez, Pedro Pablo IR - Oyaguez PP FIR - Rodriguez Fanjul, Xavier IR - Rodriguez Fanjul X FIR - Gual Sanchez, Maria IR - Gual Sanchez M FIR - Joyanes Abacens, Belen IR - Joyanes Abacens B FIR - Ots Ruiz, Elsa IR - Ots Ruiz E FIR - Holanda Pena, M feminine Soledad IR - Holanda Pena MS FIR - Coca Perez, Ana IR - Coca Perez A FIR - Diaz Vico, Ana IR - Diaz Vico A FIR - Martinez Padilla, M feminine Carmen IR - Martinez Padilla MC FIR - Alonso-Grana Lopez-Manteola, Sira IR - Alonso-Grana Lopez-Manteola S FIR - Mendizabal, Mikel IR - Mendizabal M FIR - Torres, Maria Amores IR - Torres MA FIR - Leon Gonzalez, Jose Sebastian IR - Leon Gonzalez JS FIR - Ortiz Pallares, Manuel IR - Ortiz Pallares M FIR - Castells Vilella, Laura IR - Castells Vilella L FIR - Sanchez Granados, Jose Manuel IR - Sanchez Granados JM FIR - Jimenez Jimenez, Ana Belen IR - Jimenez Jimenez AB FIR - Molina, Ana Abril IR - Molina AA FIR - Gonzalez Rivera, Iria IR - Gonzalez Rivera I EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:18 PHST- 2026/05/26 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:18 [entrez] AID - 10.1007/s00431-026-07504-2 [pii] AID - 10.1007/s00431-026-07504-2 [doi] PST - epublish SO - Eur J Pediatr. 2026 Oct 28;185(11):900. doi: 10.1007/s00431-026-07504-2. PMID- 42955400 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1432-1076 (Electronic) IS - 0340-6199 (Linking) VI - 185 IP - 11 DP - 2026 Oct 29 TI - Comparison of outcomes after transcatheter patent ductus arteriosus closure in infants weighing 1.5-4 kg. LID - 905 [pii] LID - 10.1007/s00431-026-07581-3 [doi] AB - Transcatheter closure of patent ductus arteriosus (PDA) with the Amplatzer Piccolo Occluder is safe and effective in infants 1.5-4 kg groups. This retrospective, international, multicenter study included 358 infants who underwent transcatheter PDA closure at 5 tertiary centers between 2016 and 2024: 191 weighed 1.5-4 kg (group 2). Median age was 39 days (interquartile range 25-61) and median weight was 1.48 (1.1-2.2) kg; 93.6% were preterm. Procedural success was higher in group 1 (98.4% vs 94.0%; p = 0.044). Major adverse events were more frequent in group 2 (9.6% vs 3.1%; p = 0.0144; odds ratio = 3.27; 95% confidence interval, 1.25-8.55). Device embolization occurred only in group 2 (5.4% vs 0%; p < 0.001; odds ratio = 22.96; 95% confidence interval, 1.33-397.51). The Amplatzer Piccolo Occluder was used in 97.3% of group 1 vs 69.5% of group 2. CONCLUSION: Transcatheter PDA closure is safe and effective in infants 1.5-4 kg group has a higher risk of major adverse events, particularly device embolization. Better patient selection and dedicated devices may improve safety. WHAT IS KNOWN: * Transcatheter PDA closure with the Amplatzer Piccolo Occluder is safe and effective in very small infants. * In larger infants, alternative devices are often used off-label, but evidence on procedural outcomes remains limited. WHAT IS NEW: * Infants weighing >1.5-4 kg had lower procedural success and more major adverse events than those weighing 1.5-4 kg group, supporting careful anatomical assessment and the development of dedicated devices. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Librandi, Katia AU - Librandi K AD - Pediatric Cardiology, Royal Brompton & Harefield NHS Foundation Trust, Sydney Street, London, SW3 6NP, UK. AD - Cardiology, Cardiac Surgery and Heart Lung Transplantation, Bambino Gesu Hospital and Research Institute, IRCCS, Rome, Italy. FAU - Bautista-Rodriguez, Carles AU - Bautista-Rodriguez C AD - Pediatric Cardiology, Royal Brompton & Harefield NHS Foundation Trust, Sydney Street, London, SW3 6NP, UK. FAU - Butera, Gianfranco AU - Butera G AD - Cardiology, Cardiac Surgery and Heart Lung Transplantation, Bambino Gesu Hospital and Research Institute, IRCCS, Rome, Italy. FAU - Annoni, Giuseppe AU - Annoni G AD - Pediatric Cardiology, Regina Margherita Children Hospital, Piazza Polonia 96, Turin, Italy. AD - Pediatric Cardiology, Grande Ospedale Metropolitano Niguarda, Milan, Italy. FAU - Padovani, Paul AU - Padovani P AD - Department of Pediatric Cardiology and Pediatric Cardiac Surgery, FHU PRECICARE, Nantes Universite, CHU Nantes, 44000, Nantes, France. FAU - Foldvari, Sandrine AU - Foldvari S AD - Pediatric Cardiology, Royal Brompton & Harefield NHS Foundation Trust, Sydney Street, London, SW3 6NP, UK. FAU - Baruteau, Alban-Elouen AU - Baruteau AE AD - Department of Pediatric Cardiology and Pediatric Cardiac Surgery, FHU PRECICARE, Nantes Universite, CHU Nantes, 44000, Nantes, France. AD - Department of Pediatric Cardiology and Pediatric Cardiac Surgery, CIC 1413, INSERM, Nantes Universite, CHU Nantes, 44000, Nantes, France. AD - INRAE UMR 1280 PhAN, Nantes Universite, CHU Nantes, 44000, Nantes, France. AD - l'institut du thorax, CNRS, INSERM, Nantes Universite, CHU Nantes, 44000, Nantes, France. FAU - Fraisse, Alain AU - Fraisse A AD - Pediatric Cardiology, Royal Brompton & Harefield NHS Foundation Trust, Sydney Street, London, SW3 6NP, UK. alain.fraisse@nhs.net. LA - eng PT - Comparative Study PT - Journal Article PT - Multicenter Study DEP - 20261029 PL - Germany TA - Eur J Pediatr JT - European journal of pediatrics JID - 7603873 SB - IM MH - Humans MH - *Ductus Arteriosus, Patent/surgery MH - Retrospective Studies MH - *Septal Occluder Device/adverse effects MH - *Cardiac Catheterization/methods/adverse effects MH - Treatment Outcome MH - Female MH - Male MH - Infant MH - Infant, Newborn OTO - NOTNLM OT - Device embolization OT - Interventional congenital cardiology OT - Patent ductus arteriosus OT - Procedural outcomes OT - Transcatheter PDA closure COIS- Declarations. Ethics approval: The requirement for formal ethics committee approval was waived because of the retrospective nature of the study and the use of anonymized routinely collected clinical data. Consent to participate: The study was conducted in accordance with the ethical principles of the Declaration of Helsinki. The requirement for individual informed consent was waived because of the retrospective nature of the study and the use of anonymized data. Consent for publication: Not applicable. Competing interests: Alban-Elouen Baruteau and Alain Fraisse are consultants and proctors for Abbott and Occlutech Inc. The other authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:18 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:18 [entrez] AID - 10.1007/s00431-026-07581-3 [pii] AID - 10.1007/s00431-026-07581-3 [doi] PST - epublish SO - Eur J Pediatr. 2026 Oct 29;185(11):905. doi: 10.1007/s00431-026-07581-3. PMID- 42955393 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1432-0827 (Electronic) IS - 0171-967X (Linking) VI - 117 IP - 1 DP - 2026 Oct 29 TI - Predictors of Major Incident Fracture During Adulthood: Evidence from the Geelong Osteoporosis Study. LID - 172 [pii] LID - 10.1007/s00223-026-01638-z [doi] AB - As people age, preventing fractures becomes crucial for preserving independence and overall quality of life. This study aimed to identify factors associated with reduced hazard of major incident fractures during adulthood. Serial data from 1394 females and 1404 males (ages 20-96y) participating in the Geelong Osteoporosis Study were analysed. Fractures (hip, spine, proximal humerus, distal radius, pelvis) were identified through radiological reports. Sociodemographic and lifestyle factors, Anthropometry, dual-energy X-ray absorptiometry, comorbidities, medication use and biochemical markers were collected across the visits. Predictors of major incident fractures were examined using time-dependent Cox proportional hazards models with age as the primary time scale. Participants were followed from baseline until first fracture, death, loss to follow-up or 31/12/2020. Stepwise forward selection identified relevant predictors, and missing data were imputed using predictive mean matching. Over a median follow-up of 16.3y (IQR:10.5-23.5), fractures occurred in 430 participants (15.4%). Males had a lower fracture hazard than females (HR 0.71, 95% CI 0.52-0.97). Factors associated with a reduced hazard of fracture included never-smoking (0.70, 0.50-0.97), past smokers (0.67, 0.46-0.99), higher hip and spine T-scores (0.82, 0.72-0.93; 0.82, 0.76-0.89), lower plasma glucose (0.60, 0.42-0.87), lower HDL cholesterol (0.69, 0.52-0.90), absence of falls (0.74, 0.59-0.91), no muscle weakness (0.48, 0.23-0.98) and non-use of oral glucocorticoids (0.48, 0.31-0.75). Preserving bone mass, undertaking falls prevention strategies, avoiding smoking, monitoring oral glucocorticoid use and optimising metabolic health could be possible strategies for reducing fracture risk during ageing. The independent contribution of HDL might provide new perspectives into fracture risk assessment. CI - (c) 2026. The Author(s). FAU - Suleiman, Aminu AU - Suleiman A AUID- ORCID: 0000-0002-4732-4454 AD - School of Medicine, Deakin Institute for Mental and Physical Health and Clinical Translation (Deakin IMPACT), School of Medicine, Deakin University, Geelong, VIC, 3220, Australia. a.suleiman@deakin.edu.au. FAU - Lotfaliany, Mojtaba AU - Lotfaliany M AUID- ORCID: 0000-0001-6594-9004 AD - School of Medicine, Deakin Institute for Mental and Physical Health and Clinical Translation (Deakin IMPACT), School of Medicine, Deakin University, Geelong, VIC, 3220, Australia. FAU - Williams, Lana J AU - Williams LJ AUID- ORCID: 0000-0002-1377-1272 AD - School of Medicine, Deakin Institute for Mental and Physical Health and Clinical Translation (Deakin IMPACT), School of Medicine, Deakin University, Geelong, VIC, 3220, Australia. AD - Barwon Health, Geelong, VIC, 3220, Australia. FAU - Anderson, Kara B AU - Anderson KB AUID- ORCID: 0000-0002-7798-180X AD - School of Medicine, Deakin Institute for Mental and Physical Health and Clinical Translation (Deakin IMPACT), School of Medicine, Deakin University, Geelong, VIC, 3220, Australia. FAU - Stuart, Amanda L AU - Stuart AL AUID- ORCID: 0000-0001-8770-9511 AD - School of Medicine, Deakin Institute for Mental and Physical Health and Clinical Translation (Deakin IMPACT), School of Medicine, Deakin University, Geelong, VIC, 3220, Australia. FAU - Holloway-Kew, Kara L AU - Holloway-Kew KL AUID- ORCID: 0000-0001-5064-2990 AD - School of Medicine, Deakin Institute for Mental and Physical Health and Clinical Translation (Deakin IMPACT), School of Medicine, Deakin University, Geelong, VIC, 3220, Australia. FAU - Pasco, Julie A AU - Pasco JA AUID- ORCID: 0000-0002-8968-4714 AD - School of Medicine, Deakin Institute for Mental and Physical Health and Clinical Translation (Deakin IMPACT), School of Medicine, Deakin University, Geelong, VIC, 3220, Australia. AD - Barwon Health, Geelong, VIC, 3220, Australia. AD - Department of Epidemiology and Preventive Medicine, Monash University, Melbourne, VIC, 3220, Australia. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Calcif Tissue Int JT - Calcified tissue international JID - 7905481 SB - IM MH - Humans MH - Male MH - Female MH - Aged MH - Risk Factors MH - *Osteoporosis/epidemiology/complications MH - Middle Aged MH - Aged, 80 and over MH - Adult MH - Bone Density/physiology MH - *Osteoporotic Fractures/epidemiology MH - *Fractures, Bone/epidemiology MH - Incidence MH - Young Adult OTO - NOTNLM OT - Healthy ageing OT - Incident fractures OT - Musculoskeletal ageing OT - Time-dependent Cox regression COIS- Declarations. Conflict of interest: The authors declare no competing interests. Ethical Approval: This study involved human participants and is in compliance with Deakin University and Barwon Health ethical standards. The study was approved by the Barwon Health Human Research Ethics Committee, with the approval numbers ID 92/01 and ID 00/56. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:18 PHST- 2026/02/16 00:00 [received] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:18 [entrez] AID - 10.1007/s00223-026-01638-z [pii] AID - 10.1007/s00223-026-01638-z [doi] PST - epublish SO - Calcif Tissue Int. 2026 Oct 29;117(1):172. doi: 10.1007/s00223-026-01638-z. PMID- 42955392 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1590-3478 (Electronic) IS - 1590-1874 (Linking) VI - 47 IP - 11 DP - 2026 Oct 28 TI - Effects of STN-DBS on voluntary facial mimicry in Parkinson's patients: a pilot study. LID - 894 [pii] LID - 10.1007/s10072-026-09512-4 [doi] AB - PURPOSE: Parkinson disease (PD) causes hypomimia, an early manifestation driven by motor, limbic and basal ganglia dysfunction that impairs nonverbal communication. Traditional clinical rating scales used to assess facial expressivity are subjective, traditional clinical scales are subjective, whereas automated video analysis based on the Facial Action Coding System (FACS) provides an objective, fine-grained measurement of specific facial Action Units (AUs). Subthalamic Deep Brain Stimulation (STN-DBS), a widely adopted therapy for PD, modulates basal ganglia circuits, yet its precise effect on facial mimicry remains poorly characterized. This study evaluates how STN-DBS influences voluntary facial expressivity by comparing Stim-ON/Med-ON and Stim-OFF/Med-ON states. METHODS: 18 PD-patients with STN-DBS were evaluated in two experimental conditions (Stim-ON/Med-ON VS Stim-OFF/Med-ON). Video recordings captured participants reproducing seven voluntary facial expressions (six basic emotions plus neutral). Facial mimicry was quantified using OpenFace 2.0 to extracts AUs intensities according to the FACS system. RESULTS: MDS-UPDRS-III scores improved significantly in the DBS-ON state (p < 0.001), confirming the expected improvement in motor symptoms. Automated FACS analysis revealed emotion-specific differences in AUs, showing reduced mimicry in the Stim-OFF/Med-ON condition, for neutral (AU06, p = 0.042), happiness (AU06, p = 0.034), fear (AU25, p = 0.043), and anger (AU23, p = 0.036). For sadness, AU09 activation was higher in the OFF state (p = 0.021). No significant differences emerged for disgust or surprise. CONCLUSION: STN-DBS appears to selectively modulate voluntary facial mimicry in PD, enhancing activation of emotion-relevant AUs, and reducing non-pertinent activations for sadness. These findings indicate that STN-DBS influences emotional motor output beyond classical motor control, potentially benefiting nonverbal social interaction. CI - (c) 2026. Fondazione Societa Italiana di Neurologia. FAU - Scagliotti, E AU - Scagliotti E AUID- ORCID: 0009-0004-9102-7547 AD - Unit of Neurosurgery, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, 20122, Italy. FAU - Pirola, E AU - Pirola E AD - Unit of Neurosurgery, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, 20122, Italy. FAU - Remore, L G AU - Remore LG AUID- ORCID: 0000-0003-4118-9009 AD - Unit of Neurosurgery, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, 20122, Italy. luigigianmaria.remore@gmail.com. AD - Department of Pathophysiology and Transplantation, University of Milan, Milan, 20122, Italy. luigigianmaria.remore@gmail.com. FAU - Ampollini, A AU - Ampollini A AD - Unit of Neurosurgery, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, 20122, Italy. FAU - Fornara, G Abete AU - Fornara GA AD - Unit of Neurosurgery, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, 20122, Italy. FAU - Ferrucci, R AU - Ferrucci R AD - Unit of Neurophysiopathology, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, 20122, Italy. AD - Department of Oncology and Hematology-Oncology, University of Milan, Milan, 20122, Italy. FAU - Mameli, F AU - Mameli F AD - Unit of Neurophysiopathology, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, 20122, Italy. FAU - Zirone, E AU - Zirone E AD - Unit of Neurophysiopathology, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, 20122, Italy. FAU - Cogiamanian, F AU - Cogiamanian F AD - Unit of Neurophysiopathology, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, 20122, Italy. FAU - Mailland, E AU - Mailland E AD - Unit of Neurophysiopathology, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, 20122, Italy. FAU - Luiso, F AU - Luiso F AD - Parkinson Institute of Milano ASST G.Pini-CTO, Milan, Italy. AD - University Hospital Wurzburg and Julius Maximilian University of Wurzburg, Wurzburg, Germany. FAU - Isaias, I U AU - Isaias IU AD - Parkinson Institute of Milano ASST G.Pini-CTO, Milan, Italy. AD - University Hospital Wurzburg and Julius Maximilian University of Wurzburg, Wurzburg, Germany. FAU - Locatelli, M AU - Locatelli M AD - Unit of Neurosurgery, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, 20122, Italy. AD - Department of Pathophysiology and Transplantation, University of Milan, Milan, 20122, Italy. LA - eng GR - RC2023-235/Ministero dell'Istruzione, dell'Universita e della Ricerca/ GR - RC2024-400/Ministero dell'Istruzione, dell'Universita e della Ricerca/ PT - Journal Article DEP - 20261028 PL - Italy TA - Neurol Sci JT - Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology JID - 100959175 SB - IM MH - Humans MH - *Parkinson Disease/therapy/physiopathology/psychology MH - Male MH - Pilot Projects MH - *Facial Expression MH - Female MH - Middle Aged MH - Aged MH - *Deep Brain Stimulation/methods MH - *Subthalamic Nucleus/physiology MH - *Imitative Behavior/physiology OTO - NOTNLM OT - Facial expressivity OT - Facial mimicry OT - Hypomimia OT - Parkinson's disease OT - Subthalamic deep brain stimulation COIS- Declarations. Ethics approval: Ethical approval was obtained by the Ethical Committee "Milano Area 2" (ID number 5293). Consent: Informed consent was obtained from all individual participants included in the study. Competing interests: Authors LGR and ML received travel reimbursements from Medtronic Inc., but declare no financial or non-financial competing interests. Author EP received travel reimbursements from Abbot, but declares no financial or non-financial competing interests. Author FC is a shareholder of Newronika S.p.A. Author IUI received lecture honoraria and research funding from Medtronic Inc. and Boston Scientific, but declares no financial or non-financial competing interests. Author IUI is a consultant to and shareholder of Newronika S.p.A. and received funding for research activities from Newronika S.p.A., a spin-off company of the University of Milan and the Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, but declares no financial or non-financial competing interests. Author IUI serves as Adjunct Professor at the NYU Grossman School of Medicine and the University of Milan, but declares no financial or non-financial competing interests. Other authors declare no financial or non-financial competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:18 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:18 [entrez] AID - 10.1007/s10072-026-09512-4 [pii] AID - 10.1007/s10072-026-09512-4 [doi] PST - epublish SO - Neurol Sci. 2026 Oct 28;47(11):894. doi: 10.1007/s10072-026-09512-4. PMID- 42955390 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1432-2048 (Electronic) IS - 0032-0935 (Linking) VI - 264 IP - 6 DP - 2026 Oct 28 TI - Evolutionary origin of the WHIRLY gene families across 330 plant species: from algae to land plants. LID - 180 [pii] LID - 10.1007/s00425-026-05187-5 [doi] AB - Phylogenomics of 921 WHIRLY genes across 330 plants reveals stepwise subfamily origins, purifying selection, and a novel AtWHY1/2/3 hetero-oligomeric stress complex. Whirly (WHY) transcription factors are a plant-specific, highly conserved family with central roles in organellar genome maintenance, retrograde signaling, and abiotic stress response, but their deep evolutionary trajectories across the full green plant lineage have remained largely unresolved. In the present study, 921 WHY genes were identified across 330 different species from algae to land plants, which were classified into three subfamilies (WHY I, WHY II, and WHY III) in this study. Structural characteristics of different subfamilies showed significant differences in the number of exons and protein motifs, which may have arisen due to functional differentiation. Collinearity was primarily observed among members of the same subfamily, and network clustering analysis further divided all WHY genes into three clusters, with genes within each cluster exhibiting significant collinearity. All three subfamilies are under intense long-term purifying selection, with average Ka/Ks ratios far below 1, indicating stringent evolutionary constraints on their core conserved single-stranded DNA binding domain. Using yeast two-hybrid assays, protein molecular docking and site-specific interaction validation, we further uncovered a previously unreported stable hetero-oligomeric complex formed by AtWHY1, AtWHY2, and AtWHY3, assembled via defined hydrogen bond networks and salt bridges at the conserved interface residues, which acts as a combinatorial regulatory switch to coordinate distinct subcellular stress responses. This work establishes a unified evolutionary framework for the WHY family and provides new mechanistic insights into how their hetero-oligomerization drives specialized stress adaptation during plant territorialization. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Lan, Yanhong AU - Lan Y AD - Key Laboratory of Germplasm Innovation & Utilization of Horticulture Crops of Sichuan Province, Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China. AD - Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement in Southwest Regions, Ministry of Agriculture and Rural Affairs of the P.R. China, Chengdu, 610066, China. AD - Sichuan Province Engineering Technology Research Center of Vegetables, Chengdu, 611934, China. FAU - Gong, Fangyi AU - Gong F AD - Key Laboratory of Germplasm Innovation & Utilization of Horticulture Crops of Sichuan Province, Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China. AD - Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement in Southwest Regions, Ministry of Agriculture and Rural Affairs of the P.R. China, Chengdu, 610066, China. AD - Sichuan Province Engineering Technology Research Center of Vegetables, Chengdu, 611934, China. FAU - Dong, Rongjiang AU - Dong R AD - Key Laboratory of Germplasm Innovation & Utilization of Horticulture Crops of Sichuan Province, Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China. FAU - Fang, Chao AU - Fang C AD - Key Laboratory of Germplasm Innovation & Utilization of Horticulture Crops of Sichuan Province, Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China. Fangchao@scsaas.cn. AD - Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement in Southwest Regions, Ministry of Agriculture and Rural Affairs of the P.R. China, Chengdu, 610066, China. Fangchao@scsaas.cn. AD - Sichuan Province Engineering Technology Research Center of Vegetables, Chengdu, 611934, China. Fangchao@scsaas.cn. FAU - Cai, Peng AU - Cai P AUID- ORCID: 0009-0005-7678-7609 AD - Key Laboratory of Germplasm Innovation & Utilization of Horticulture Crops of Sichuan Province, Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China. cp215@scsaas.cn. AD - Key Laboratory of Horticultural Crops Biology and Germplasm Enhancement in Southwest Regions, Ministry of Agriculture and Rural Affairs of the P.R. China, Chengdu, 610066, China. cp215@scsaas.cn. AD - Sichuan Province Engineering Technology Research Center of Vegetables, Chengdu, 611934, China. cp215@scsaas.cn. LA - eng GR - grant No.1+9KJGG03/the 1+9 Program of SAAS/ GR - grant No. SCCXTD-2024-5/Sichuan Innovation Team of National Modern Agricultural Industry Technology System/ GR - Grant no: 2022NSFSC0934/Sichuan Provincial Science and Technology Plan Project/ GR - 2023YFG0122/Sichuan Provincial Science and Technology Plan Project/ PT - Journal Article DEP - 20261028 PL - Germany TA - Planta JT - Planta JID - 1250576 RN - 0 (Plant Proteins) RN - 0 (Transcription Factors) SB - IM MH - *Evolution, Molecular MH - Phylogeny MH - *Multigene Family/genetics MH - *Plant Proteins/genetics/metabolism MH - *Transcription Factors/genetics/metabolism MH - *Embryophyta/genetics MH - *Plants/genetics MH - *Genes, Plant/genetics MH - Genome, Plant/genetics OTO - NOTNLM OT - Abiotic stress OT - Phylogenomic evolution OT - Protein interaction OT - Purifying selection OT - Synteny network OT - Whirly transcription factor COIS- Declarations. Conflicts of interest: The authors have no financial conflicts of interest to declare. Informed consent: Not applicable. Institutional review board statement: Not applicable. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:18 PHST- 2026/04/01 00:00 [received] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:18 [entrez] AID - 10.1007/s00425-026-05187-5 [pii] AID - 10.1007/s00425-026-05187-5 [doi] PST - epublish SO - Planta. 2026 Oct 28;264(6):180. doi: 10.1007/s00425-026-05187-5. PMID- 42955389 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1436-5073 (Electronic) IS - 0026-3672 (Linking) VI - 193 IP - 11 DP - 2026 Oct 28 TI - Eu-MOF-based sandwich-type magnetic molecularly imprinted fluorescent sensor for sensitive and visual detection of chlorogenic acid in cigar tobacco. LID - 768 [pii] LID - 10.1007/s00604-026-08497-3 [doi] AB - A sandwich-type magnetic molecularly imprinted fluorescent sensor (BA-Eu-MOF-CGA-MIPs) based on europium-based metal-organic frameworks (Eu-MOF) was constructed for highly selective detection of chlorogenic acid (CGA) in cigar tobacco and its products. The sensor integrates magnetic separation (via Fe(3)O(4) nanoparticles), imprinted cavities for specific capture, and boronate-affinity-mediated sandwich structure for fluorescence quenching. Under optimal conditions, a detection limit of 0.96 microg/mL and a linear range of 21.3-173 microg/mL were achieved. The sensor exhibits excellent selectivity over structural analogs and enables smartphone-assisted visual semi-quantitative detection. Satisfactory recoveries ranging from 93.74% to 104.07% were obtained in real cigar samples (Cigar No. 39 and Cigar No. 1) as well as flue-cured tobacco varieties, demonstrating its great potential for quality control in the cigar industry, including raw material screening, fermentation monitoring, and product uniformity evaluation. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature. FAU - Pang, Tao AU - Pang T AD - Yunnan Academy of Tobacco Agricultural Science, Kunming, 650021, China. FAU - Li, Yong AU - Li Y AD - Yunnan Academy of Tobacco Agricultural Science, Kunming, 650021, China. liyong189108@163.com. FAU - Yang, Qingqing AU - Yang Q AD - Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, China. FAU - Cai, Changqun AU - Cai C AD - Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, China. FAU - Gong, Hang AU - Gong H AUID- ORCID: 0000-0003-4513-593X AD - Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, 411105, China. hgong@xtu.edu.cn. AD - Yunnan Key Laboratory of Modern Separation Analysis and Substance Transformation, Engineering Research Center for Valorization of Unique Bio-Resources in Yunnan, Ministry of Education, College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming, 650500, China. hgong@xtu.edu.cn. LA - eng GR - 2024530000241028/China National Tobacco Company Yunnan branch/ GR - 2026JJ50125/Natural Science Foundation of Hunan Province/ GR - 202449CE340001/Science and Technology Program of Yunnan Province/ PT - Journal Article DEP - 20261028 PL - Austria TA - Mikrochim Acta JT - Mikrochimica acta JID - 7808782 RN - 318ADP12RI (Chlorogenic Acid) RN - 444W947O8O (Europium) RN - 0 (Fluorescent Chemosensor Compounds) RN - 0 (Fluorescent Dyes) RN - 0 (Magnetite Nanoparticles) SB - IM MH - *Chlorogenic Acid/analysis/chemistry MH - *Molecular Imprinting/methods MH - *Europium/chemistry MH - Limit of Detection MH - Fluorescent Chemosensor Compounds MH - *Fluorescent Dyes/chemistry MH - Spectrometry, Fluorescence/methods MH - *Tobacco Products/analysis MH - *Nicotiana/chemistry MH - Magnetite Nanoparticles/chemistry OTO - NOTNLM OT - Boronate affinity OT - Chlorogenic acid OT - Cigar tobacco leaves OT - Fluorescent sensor OT - Magnetic separation OT - Molecularly imprinted polymers OT - Smartphone-based detection COIS- Declarations. Declaration of AI use: During the preparation of this work, the authors did not use any artificial intelligence (AI) tools or large language models for data analysis, figure generation, or manuscript writing. All figures and graphical elements were created based on experimental results using standard software, and the text was written and revised entirely by the authors. The authors take full responsibility for the content and originality of this work. Competing interests: The authors declare no competing interests. Conflict of interest: The authors have no relevant financial or non-financial interests to disclose. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:18 PHST- 2026/08/13 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:18 [entrez] AID - 10.1007/s00604-026-08497-3 [pii] AID - 10.1007/s00604-026-08497-3 [doi] PST - epublish SO - Mikrochim Acta. 2026 Oct 28;193(11):768. doi: 10.1007/s00604-026-08497-3. PMID- 42955384 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1432-1459 (Electronic) IS - 0340-5354 (Linking) VI - 273 IP - 11 DP - 2026 Oct 29 TI - When gait reveals emotion: recognition through gait videos in Parkinson's disease. LID - 691 [pii] LID - 10.1007/s00415-026-14239-5 [doi] AB - INTRODUCTION: Gait disturbances represent a hallmark of Parkinson's disease (PD), yet gait is increasingly recognized as not solely a motor function but as a dynamic channel for emotional expression. PD patients might exhibit deficits in recognizing emotions and emotional blunting, characterized by reduced valence and intensity ratings. However, whether individuals with PD can perceive emotional states from human gait remains unexplored. OBJECTIVES: This study examined whether PD individuals can recognize emotions from gait videos and whether they show selective impairments in emotional appraisal, with respect to healthy controls (HC) matched for age and sex. METHODS: Thirty-eight individuals with PD and 38 HC completed an online questionnaire including 12 videos of an actress walking with gait patterns expressing six emotions (i.e., happiness, sadness, fear, anxiety, anger, and neutral), each shown with blurred or visible facial expressions. Participants identified the expressed emotion and rated its valence and intensity. Accuracy in emotion recognition and valence and intensity scores were compared between and within groups and between conditions (i.e., blurred-face vs facial expression). RESULTS: Recognition accuracy and valence ratings were largely preserved in PD. PD participants reported lower overall emotional intensity than HC, without evidence of an emotion-specific reduction. CONCLUSIONS: PD patients display reduced emotional appraisal regarding intensity yet generally retain the ability to recognize emotions and their valence from gait. These findings may inform future research on the potential integration of emotional gait stimuli into action observation training programs. CI - (c) 2026. Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Putzolu, Martina AU - Putzolu M AD - Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics and Maternal Child Health, University of Genoa, Genoa, Italy. AD - Azienda Ospedaliera Metropolitana (AOM), IRCCS Ospedale Policlinico San Martino, Genoa, Italy. FAU - Sarasso, Elisabetta AU - Sarasso E AD - Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics and Maternal Child Health, University of Genoa, Genoa, Italy. AD - Neuroimaging Research Unit, Institute of Experimental Neuroscience, IRCCS San Raffaele Scientific Institute, Via Olgettina, 60, 20132, Milan, Italy. AD - Neurotech Hub, Vita-Salute San Raffaele University, Milan, Italy. FAU - Canu, Elisa AU - Canu E AD - Neuroimaging Research Unit, Institute of Experimental Neuroscience, IRCCS San Raffaele Scientific Institute, Via Olgettina, 60, 20132, Milan, Italy. AD - Neurotech Hub, Vita-Salute San Raffaele University, Milan, Italy. FAU - Gardoni, Andrea AU - Gardoni A AD - Neuroimaging Research Unit, Institute of Experimental Neuroscience, IRCCS San Raffaele Scientific Institute, Via Olgettina, 60, 20132, Milan, Italy. AD - Neurotech Hub, Vita-Salute San Raffaele University, Milan, Italy. FAU - Terranova, Sara AU - Terranova S AD - Neurotech Hub, Vita-Salute San Raffaele University, Milan, Italy. FAU - Simeon, Rachele AU - Simeon R AD - Neurotech Hub, Vita-Salute San Raffaele University, Milan, Italy. FAU - Romano, Veronica AU - Romano V AD - Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics and Maternal Child Health, University of Genoa, Genoa, Italy. FAU - Basaia, Silvia AU - Basaia S AD - Neuroimaging Research Unit, Institute of Experimental Neuroscience, IRCCS San Raffaele Scientific Institute, Via Olgettina, 60, 20132, Milan, Italy. AD - Neurotech Hub, Vita-Salute San Raffaele University, Milan, Italy. FAU - Avanzino, Laura AU - Avanzino L AD - Azienda Ospedaliera Metropolitana (AOM), IRCCS Ospedale Policlinico San Martino, Genoa, Italy. AD - Department of Experimental Medicine, Section of Human Physiology, University of Genoa, Genoa, Italy. FAU - Filippi, Massimo AU - Filippi M AD - Neuroimaging Research Unit, Institute of Experimental Neuroscience, IRCCS San Raffaele Scientific Institute, Via Olgettina, 60, 20132, Milan, Italy. AD - Neurotech Hub, Vita-Salute San Raffaele University, Milan, Italy. AD - Neurology Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy. AD - Neurorehabilitation Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy. AD - Neurophysiology Service, IRCCS San Raffaele Scientific Institute, Milan, Italy. FAU - Agosta, Federica AU - Agosta F AUID- ORCID: 0000-0003-3121-4979 AD - Neuroimaging Research Unit, Institute of Experimental Neuroscience, IRCCS San Raffaele Scientific Institute, Via Olgettina, 60, 20132, Milan, Italy. agosta.federica@hsr.it. AD - Neurotech Hub, Vita-Salute San Raffaele University, Milan, Italy. agosta.federica@hsr.it. AD - Neurology Unit, IRCCS San Raffaele Scientific Institute, Milan, Italy. agosta.federica@hsr.it. FAU - Pelosin, Elisa AU - Pelosin E AD - Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics and Maternal Child Health, University of Genoa, Genoa, Italy. AD - Azienda Ospedaliera Metropolitana (AOM), IRCCS Ospedale Policlinico San Martino, Genoa, Italy. LA - eng GR - MNESYS (PE0000006)/Ministero dell'Universita e della Ricerca/ PT - Journal Article DEP - 20261029 PL - Germany TA - J Neurol JT - Journal of neurology JID - 0423161 SB - IM MH - Humans MH - *Parkinson Disease/complications/physiopathology/psychology MH - Male MH - Female MH - Aged MH - Middle Aged MH - *Emotions/physiology MH - *Gait Disorders, Neurologic/etiology/physiopathology MH - Facial Expression MH - *Gait/physiology MH - *Recognition, Psychology/physiology MH - Video Recording OTO - NOTNLM OT - Embodiment OT - Emotion OT - Gait OT - Intensity OT - Parkinson's disease OT - Valence COIS- Declarations. Conflicts of interest: M.P. work was partially supported by #NEXTGENERATIONEU (NGEU) and partially funded by the Ministry of University and Research (MUR), National Recovery and Resilience Plan (NRRP), project MNESYS (PE0000006)-A Multiscale integrated approach to the study of the nervous system in health and disease (DN. 1553 11.10.2022). E.S., E.C. and S.B. have received research support from the Italian Ministry of Health. A.G., S.T., R.S., and V.R. declare no financial conflicts of interest. L.A. has received speaker honoraria from Zambon and Bial and received research supports (Grants) from the EU Joint Programme for Neurodegenerative Disease Research (JPND) (2022 call) and Michael J. Fox Foundation (Fall 2022 biomarkers to support therapeutic trials program). She received research support from FRESCO foundation and the Italian Ministry of Health (Ricerca Finalizzata 2022 and 5X1000). M.F. is Editor-in-Chief of the Journal of Neurology, Associate Editor of Human Brain Mapping, Neurological Sciences, and Radiology; received compensation for consulting services from Almirall, Biogen, Bristol-Myers Squibb, Eli Lilly, Merck, Novartis, Roche, Sanofi; speaking activities from Amgen, Bayer, Biogen, Bristol-Myers Squibb, Celgene, Chiesi Italia SpA, Eisai, Eli Lilly, Fujirebio, Genzyme, Janssen, Merck, Neopharmed Gentili, Neuraxpharm, Novartis, Novo Nordisk, Roche, Sanofi, Takeda; participation in Advisory Boards for Alexion, Biogen, Bristol-Myers Squibb, Eli Lilly, GE Healthcare Ltd, Merck, Neuraxpharm, Novartis, Roche, Sandoz, Sanofi, Takeda; scientific direction of educational events for Biogen, Merck, Roche, Celgene, Bristol-Myers Squibb, Lilly, Novartis, Sanofi-Genzyme; he receives research support from Biogen Idec, Merck-Serono, Novartis, Roche, the Italian Ministry of Health, the Italian Ministry of University and Research, and Fondazione Italiana Sclerosi Multipla. F.A. is Associate Editor of NeuroImage: Clinical, has received speaker honoraria from Biogen Idec, Italfarmaco, Roche, Zambon, Eli Lilly, GE Healthcare and Bristol Myers Squibb, and receives or has received research supports from the Italian Ministry of Health, the Italian Ministry of University and Research, AriSLA (Fondazione Italiana di Ricerca per la SLA), the European Research Council, the EU Joint Programme-Neurodegenerative Disease Research (JPND) and Foundation Research on Alzheimer Disease (France). E.P. is part of an Advisory Board for M.J. Fox Foundation. She has received grants from Italian Ministry of University and Research (PRIN 2022) and Michael J. Fox Foundation (Fall 2022 biomarkers to support therapeutic trials program), and research supports from Italian Ministry of Health (Ricerca Finalizzata 2022 and 5X1000). Ethical approval: The study was approved by the institutional ethical committee of the University of Genova (CERA 2023/02). Written informed consent was obtained from all participants prior to the experimental session, and the study was conducted adhering to the Declaration of Helsinki standards. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:17 PHST- 2026/08/03 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/21 00:00 [revised] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:17 [entrez] AID - 10.1007/s00415-026-14239-5 [pii] AID - 10.1007/s00415-026-14239-5 [doi] PST - epublish SO - J Neurol. 2026 Oct 29;273(11):691. doi: 10.1007/s00415-026-14239-5. PMID- 42955382 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1434-3916 (Electronic) IS - 0936-8051 (Linking) VI - 146 IP - 1 DP - 2026 Oct 29 TI - Novel bicolumnar fixation with primary cup implantation for pelvic discontinuity in revision hip arthroplasty: a comparative clinical study. LID - 385 [pii] LID - 10.1007/s00402-026-06424-y [doi] AB - BACKGROUND: Pelvic discontinuity is a rare but challenging condition in revision hip arthroplasty. Traditional cage-based reconstruction techniques often rely solely on mechanical fixation and may be associated with implant migration, loosening, and mechanical failure. Alternative strategies aimed at restoring pelvic continuity and achieving biological fixation may improve construct stability and clinical outcomes. METHODS: This retrospective study included 19 patients with intraoperatively confirmed pelvic discontinuity who underwent revision total hip arthroplasty between 2014 and 2024. Patients were divided into three groups according to the reconstruction technique used: Group 1 (n = 8) underwent anti-protrusio cage reconstruction alone, Group 2 (n = 6) received additional anterior quadrilateral surface plating prior to cage implantation, and Group 3 (n = 5) underwent restoration of pelvic continuity through anterior and posterior column fixation followed by implantation of a porous-coated primary acetabular cup. Clinical and radiological outcomes were assessed with a minimum follow-up of 2 years, including complications, loosening, reoperation, and reoperation-free survival. RESULTS: Nineteen patients (mean age 71.2 years) with intraoperatively confirmed pelvic discontinuity were included. Aseptic loosening rates differed significantly among the three groups (P = 0.039), occurring in 50% of patients in Group 1 and in none of the patients in Groups 2 or 3. Reoperation rates were higher in Group 1 (75%) than in Group 2 (16.7%) and Group 3 (20%), although this difference did not reach statistical significance (P = 0.074). Kaplan-Meier analysis demonstrated a trend toward lower reoperation-free survival in Group 1; however, differences among the groups were not statistically significant (log-rank P = 0.237). CONCLUSION: Reconstruction strategies involving restoration of pelvic continuity through column stabilization may provide improved mechanical stability and lower complication rates compared with conventional cage-only techniques in the management of pelvic discontinuity. In selected patients, bicolumnar fixation followed by implantation of a porous-coated primary acetabular cup may offer the additional advantage of biological fixation. Larger prospective studies are needed to validate these findings. CI - (c) 2026. The Author(s). FAU - Abul, Mehmet Suleyman AU - Abul MS AD - Department of Orthopaedics and Traumatology, Baltalimani Kemik Hastaliklari Hastanesi, Istanbul, Turkey. FAU - Agir, Muzaffer AU - Agir M AD - Department of Orthopaedics and Traumatology, Acibadem University, Acibadem Maslak Hospital, International Joint Center, Istanbul, Turkey. muzafferagir@gmail.com. FAU - Yilmaz, Husnu AU - Yilmaz H AUID- ORCID: 0009-0002-3380-1181 AD - Department of Orthopaedics and Traumatology, Cermik State Hospital, Diyarbakir, Turkey. FAU - Hekim, Omer AU - Hekim O AD - Department of Orthopaedics and Traumatology, Dr. Lutfi Kirdar Sehir Hastanesi, Istanbul, Turkey. FAU - Sevim, Omer Faruk AU - Sevim OF AD - Department of Orthopaedics and Traumatology, St George's University Hospitals NHS Foundation Trust, London, UK. FAU - Tuncay, Ibrahim AU - Tuncay I AD - Department of Orthopaedics and Traumatology, Acibadem University, Acibadem Maslak Hospital, International Joint Center, Istanbul, Turkey. LA - eng PT - Comparative Study PT - Journal Article DEP - 20261029 PL - Germany TA - Arch Orthop Trauma Surg JT - Archives of orthopaedic and trauma surgery JID - 9011043 SB - IM MH - Humans MH - *Arthroplasty, Replacement, Hip/methods/instrumentation MH - *Reoperation/methods MH - Retrospective Studies MH - Female MH - Aged MH - Male MH - Prosthesis Failure MH - *Acetabulum/surgery MH - *Hip Prosthesis MH - Aged, 80 and over MH - Middle Aged OTO - NOTNLM OT - Acetabular reconstruction OT - Anti-protrusio cage OT - Pelvic discontinuity OT - Quadrilateral surface plating OT - Revision total hip arthroplasty COIS- Declarations. Conflict of interest: The authors declare that they have no conflicts of interest relevant to the content of this article. None of the authors have any financial or personal relationships with TST Orthopedics (Istanbul, Turkey), Zimmer Biomet (Warsaw, IN, USA), or any other implant manufacturer that could inappropriately influence (bias) the content of this work. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:17 PHST- 2026/06/02 00:00 [received] PHST- 2026/07/08 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:17 [entrez] AID - 10.1007/s00402-026-06424-y [pii] AID - 10.1007/s00402-026-06424-y [doi] PST - epublish SO - Arch Orthop Trauma Surg. 2026 Oct 29;146(1):385. doi: 10.1007/s00402-026-06424-y. PMID- 42955373 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1432-1459 (Electronic) IS - 0340-5354 (Linking) VI - 273 IP - 11 DP - 2026 Oct 28 TI - Changes in gait function associated with cerebral small vessel disease-related cognitive impairment during dual-task walking: a systematic review and meta-analysis. LID - 689 [pii] LID - 10.1007/s00415-026-14229-7 [doi] AB - Cerebral small vessel disease (CSVD) is a major contributor to vascular cognitive impairment. As gait abnormalities emerge early in the disease course and are closely related to cognitive decline, quantitative gait assessment offers a promising approach for the early detection of CSVD-related cognitive impairment (CSVD-CI). Dual-task (DT) gait assessment increases cognitive demands during walking and may reveal cognitive-motor deficits that are less apparent under single-task (ST) conditions. However, heterogeneity in gait metrics, imaging criteria, and cognitive paradigms has resulted in inconsistent findings, thereby impeding the identification of robust gait markers for CSVD-CI. A systematic search of PubMed, Web of Science, Scopus, and EBSCO identified nine studies reporting DT gait outcomes and eight reporting ST gait outcomes in patients with CSVD-CI, with four studies reporting outcomes under both conditions. Compared with ST conditions, DT gait assessment more effectively discriminated patients with CSVD-CI from cognitively unimpaired controls. Under DT conditions, CSVD-CI patients exhibited significantly slower gait speed (p = 0.014), greater stride time variability (p = 0.027), and higher dual-task costs (p < 0.001), suggesting a slower and less stable gait pattern. Subgroup and sensitivity analyses suggested that CSVD imaging characteristics may contribute to between-study heterogeneity, although these findings remain exploratory. These findings support further investigation of selected DT gait parameters as potential behavioral indicators of CSVD-CI. Further studies in CSVD populations defined using standardized diagnostic and inclusion criteria should evaluate the screening performance and longitudinal predictive value of DT gait parameters, as well as their associations with CI severity. CI - (c) 2026. Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Li, Tianqing AU - Li T AD - Medical School, Tianjin University, Tianjin, China. AD - State Key Laboratory of Advanced Medical Materials and Medical Devices, Tianjin University, Tianjin, China. FAU - Liu, Xiaoya AU - Liu X AUID- ORCID: 0000-0002-4361-5166 AD - Medical School, Tianjin University, Tianjin, China. liuxiaoya@tju.edu.cn. AD - Haihe Laboratory of Brain-Computer Interaction and Human-Machine Integration, Tianjin, China. liuxiaoya@tju.edu.cn. FAU - Meng, Tianyu AU - Meng T AD - Medical School, Tianjin University, Tianjin, China. AD - State Key Laboratory of Advanced Medical Materials and Medical Devices, Tianjin University, Tianjin, China. FAU - Liu, Shuang AU - Liu S AD - Medical School, Tianjin University, Tianjin, China. shuangliu@tju.edu.cn. AD - Haihe Laboratory of Brain-Computer Interaction and Human-Machine Integration, Tianjin, China. shuangliu@tju.edu.cn. FAU - Ming, Dong AU - Ming D AD - Medical School, Tianjin University, Tianjin, China. AD - State Key Laboratory of Advanced Medical Materials and Medical Devices, Tianjin University, Tianjin, China. LA - eng GR - 62376187/National Natural Science Foundation of China/ GR - 24ZXZSSS00330/Natural Science Foundation of Tianjin/ PT - Journal Article PT - Meta-Analysis PT - Review PT - Systematic Review DEP - 20261028 PL - Germany TA - J Neurol JT - Journal of neurology JID - 0423161 SB - IM MH - Humans MH - *Cerebral Small Vessel Diseases/complications/physiopathology MH - Dual-Task Tests MH - *Cognitive Dysfunction/physiopathology/etiology MH - *Gait Disorders, Neurologic/etiology/physiopathology MH - *Walking/physiology OTO - NOTNLM OT - CSVD-related cognitive impairment OT - Cerebral small vessel disease OT - Dual-task gait OT - Gait variability OT - Meta-analysis COIS- Declarations. Conflicts of interest: The authors declare no competing interests. Ethical approval: Not applicable. Consent for publication: All authors have approved this manuscript for publication. This manuscript has not previously been published and is not pending publication elsewhere. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:17 PHST- 2026/07/22 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:17 [entrez] AID - 10.1007/s00415-026-14229-7 [pii] AID - 10.1007/s00415-026-14229-7 [doi] PST - epublish SO - J Neurol. 2026 Oct 28;273(11):689. doi: 10.1007/s00415-026-14229-7. PMID- 42955366 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1432-2048 (Electronic) IS - 0032-0935 (Linking) VI - 264 IP - 6 DP - 2026 Oct 29 TI - Pseudomonas spp.-based synthetic microbial community effects on nutrient dynamics in citrus plants under induced dysbiosis. LID - 181 [pii] LID - 10.1007/s00425-026-05205-6 [doi] AB - A targeted Pseudomonas-based synthetic microbial community reshaped rhizosphere bacterial communities and altered potassium partitioning under stress conditions, suggesting a potential role in mitigating antibiotic-induced dysbiosis. Sustainable agriculture primarily relies on biological practices to improve crop yield and resilience. Synthetic bacterial communities (SynComs) are a promising approach to promote plant growth; however, their effectiveness in situ is influenced by interactions with native microbial ecosystems and by environmental stressors, such as low pH and antibiotic use. In this context, beneficial microorganisms like Pseudomonas may enhance nutrient availability and mitigate antibiotic-induced dysbiosis by supporting microbial community reassembly and functional stability, thereby improving plant health. This study evaluated the effects of a Pseudomonas-based SynCom on nutrient accumulation and rhizosphere microbial dynamics in citrus plants subjected to streptomycin-induced dysbiosis and low-pH conditions. Selected Pseudomonas strains, chosen for their compatibility and adaptability to low-pH conditions, and through whole-genome sequencing, were applied at equal concentrations (OD(600) = 0.1). SynCom treatment increased Chloroflexota abundance in the rhizosphere while reducing Actinomycetota and Pseudomonadota after 24 days. By Day 45, the abundance of Actinomycetota decreased, with slight increases in the other two phyla. At the genus level, SynCom application reduced the abundance of Pseudomonas and Agrobacterium, while enriching the Klebsiella community. Plants treated with SynCom under stress exhibited a 25% increase in leaf potassium levels, accompanied by a 29% decrease in root potassium (essential for stomatal regulation, osmotic adjustment, and photosynthetic efficiency, which are fundamental to tree growth and stress resilience) levels. Overall, our findings demonstrate that a targeted Pseudomonas-based SynCom can reshape rhizosphere communities and improve nutrient dynamics under abiotic stress, highlighting its potential as a sustainable strategy to mitigate antibiotic-induced dysbiosis and enhance citrus resilience. CI - (c) 2026. The Author(s). FAU - Ketehouli, Toi AU - Ketehouli T AD - Department of Plant Pathology, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, 32611, USA. FAU - Trub, Mason R AU - Trub MR AD - Department of Microbiology and Cell Science, University of Florida, Gainesville, FL, 32603, USA. FAU - Goss, Erica M AU - Goss EM AD - Department of Plant Pathology, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, 32611, USA. AD - Emerging Pathogens Institute, University of Florida, Gainesville, FL, 32610, USA. FAU - Martins, Samuel J AU - Martins SJ AUID- ORCID: 0000-0002-1361-3864 AD - Department of Plant Pathology, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, 32611, USA. sj.martins@ufl.edu. LA - eng GR - 2022-68015-36721/National Institute of Food and Agriculture/ PT - Journal Article DEP - 20261029 PL - Germany TA - Planta JT - Planta JID - 1250576 RN - RWP5GA015D (Potassium) RN - Y45QSO73OB (Streptomycin) RN - 0 (Anti-Bacterial Agents) SB - IM MH - *Pseudomonas/physiology MH - Rhizosphere MH - *Citrus/microbiology/metabolism MH - *Microbiota MH - Soil Microbiology MH - *Dysbiosis/chemically induced MH - Nutrients/metabolism MH - Potassium/metabolism MH - Streptomycin/pharmacology MH - Anti-Bacterial Agents/adverse effects/pharmacology MH - Plant Roots/microbiology OTO - NOTNLM OT - Antibiotic OT - Microbiome OT - Phytobiome OT - Potassium OT - Sustainable agriculture OT - Synthetic bacterial community (SynCom) COIS- Declarations. Conflict of interest: The authors declare no conflicts of interest. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:16 PHST- 2026/02/23 00:00 [received] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:16 [entrez] AID - 10.1007/s00425-026-05205-6 [pii] AID - 10.1007/s00425-026-05205-6 [doi] PST - epublish SO - Planta. 2026 Oct 29;264(6):181. doi: 10.1007/s00425-026-05205-6. PMID- 42955365 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1437-9813 (Electronic) IS - 0179-0358 (Linking) VI - 42 IP - 1 DP - 2026 Oct 28 TI - Single-center experience of pneumovesicoscopic ureteral reimplantation: clinical outcomes and surgical efficacy analysis. LID - 466 [pii] LID - 10.1007/s00383-026-06688-w [doi] AB - PURPOSE: To evaluate the clinical characteristics, surgical outcomes, and postoperative efficacy of pneumovesicoscopic ureteral reimplantation (PVUR) using the Cohen technique for primary vesicoureteral reflux (VUR) or ureterovesical junction obstruction (UVJO) and compare those from the TriNetX global health research network. METHODS: A retrospective review was conducted on patientswho underwent pneumovesicoscopic Cohen ureteral reimplantation for primary VUR grades III-V or UVJO at our institution between 2014 and 2025. Analyzed variables included dermographic data, preoperative history of urinary tract infection (UTI), operative time, estimated blood loss, length of hospital stay and duration of foley catheterization. Postoperative success was defined as resolution of reflux on follow-up imaging and the absence of postoperative febrile UTIs. RESULTS: This cohort consisted of 26 patients with a median age of 42.0 months (range 5.0-470.0 months). All procedures were completed via the pneumovesicoscopic approach without open conversion. Mean operative time was 231.7 +/- 62.4 min, with minimal blood loss in all cases. Mean length of hospital stay and foley catheterization duration were 7.1 +/- 1.5 days and 6.6 +/- 1.3 days, respectively. Follow-up voiding cystourethrography demonstrated complete resolution of VUR in 71.4% (10/14) of evaluable patients, while clinical success was achieved in 88.5% (23/26). No post-operative acute urine retention occurred. CONCLUSION: PVUR Cohen technique is a safe, effective, and reproducible minimally invasive alternative for treating VUR and UVJO. Its intravesical nature effectively preserves bladder innervation, eliminating postoperative urinary retention and ensuring superior long-term follow-up adherence compared to global real-world standards. Although bladder capacity serves as a significant predictor of operative duration, it does not compromise surgical success. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Shie, Bo-Yang AU - Shie BY AD - Department of Surgery, Taichung Veterans General Hospital, No. 1650, Sec. 4, Taiwan Boulevard, Situn District, Taichung City, 40705, Taiwan. FAU - Huang, Sheng-Yang AU - Huang SY AD - Department of Surgery, Taichung Veterans General Hospital, No. 1650, Sec. 4, Taiwan Boulevard, Situn District, Taichung City, 40705, Taiwan. AD - Department of Post-Baccalaureate Medicine, College of Medicine, National Chung Hsing University, No. 145 Xingda Rd., South Dist, Taichung City, 40227, Taiwan. AD - College of Medicine, National Yang Ming Chiao Tung University, No. 155, Sec. 2, Linong St. Beitou Dist, Taipei City, 112304, Taiwan. FAU - Su, Wei-Hsiang AU - Su WH AD - Department of Surgery, Taichung Veterans General Hospital, No. 1650, Sec. 4, Taiwan Boulevard, Situn District, Taichung City, 40705, Taiwan. FAU - Chou, Chia-Man AU - Chou CM AUID- ORCID: 0000-0003-4616-437X AD - Department of Surgery, Taichung Veterans General Hospital, No. 1650, Sec. 4, Taiwan Boulevard, Situn District, Taichung City, 40705, Taiwan. cmchou@vghtc.gov.tw. AD - Department of Post-Baccalaureate Medicine, College of Medicine, National Chung Hsing University, No. 145 Xingda Rd., South Dist, Taichung City, 40227, Taiwan. cmchou@vghtc.gov.tw. AD - College of Medicine, National Yang Ming Chiao Tung University, No. 155, Sec. 2, Linong St. Beitou Dist, Taipei City, 112304, Taiwan. cmchou@vghtc.gov.tw. LA - eng PT - Journal Article DEP - 20261028 PL - Germany TA - Pediatr Surg Int JT - Pediatric surgery international JID - 8609169 SB - IM MH - Humans MH - Retrospective Studies MH - *Ureter/surgery MH - Female MH - *Vesico-Ureteral Reflux/surgery MH - *Replantation/methods MH - Male MH - Child, Preschool MH - Treatment Outcome MH - Infant MH - Child MH - *Ureteral Obstruction/surgery MH - Length of Stay/statistics & numerical data OTO - NOTNLM OT - Outcome OT - Pediatrics OT - Pneumovesicoscopic ureteral reimplantation OT - TriNetX COIS- Declarations. Conflict of interest: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:16 PHST- 2026/08/26 00:00 [received] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:16 [entrez] AID - 10.1007/s00383-026-06688-w [pii] AID - 10.1007/s00383-026-06688-w [doi] PST - epublish SO - Pediatr Surg Int. 2026 Oct 28;42(1):466. doi: 10.1007/s00383-026-06688-w. PMID- 42955360 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1434-3916 (Electronic) IS - 0936-8051 (Linking) VI - 146 IP - 1 DP - 2026 Oct 29 TI - Preoperative coronal tibiofemoral subluxation does not affect 2-year outcomes after mobile-bearing medial unicompartmental knee arthroplasty for anteromedial osteoarthritis. LID - 387 [pii] LID - 10.1007/s00402-026-06530-x [doi] AB - INTRODUCTION: Coronal tibiofemoral subluxation (CTFS) is frequently observed in patients with medial knee osteoarthritis; however, its clinical relevance and the acceptable degree of CTFS when considering unicompartmental knee arthroplasty (UKA) remain unclear. This study aimed to determine whether preoperative CTFS influences short-term postoperative clinical outcomes in patients with anteromedial osteoarthritis (AMOA) undergoing medial UKA. METHODS: In this retrospective cohort study, 457 consecutive knees with AMOA who underwent mobile-bearing UKA performed at a single center between August 2016 and May 2023 were analyzed. CTFS, defined as medial translation of the femur relative to the tibia, was measured on preoperative weight-bearing anteroposterior radiographs and expressed as the percentage of tibial plateau width. Patients were categorized into mild (0-5%), moderate (>/= 5% to < 10%), and severe (>/= 10%) CTFS groups. Clinical outcomes were evaluated using the Oxford Knee Score (OKS) preoperatively and at 3 months, 1 and 2 years postoperatively. Pairwise comparisons of changes in OKS were performed using a general linear model with Bonferroni-adjusted post hoc tests, and adjusted mean differences were calculated controlling for age, sex, body mass index, and preoperative OKS. RESULTS: At 2 years, mean OKS improvements were 16.6 +/- 8.9, 14.8 +/- 8.3, and 16.0 +/- 9.3 in the mild, moderate, and severe CTFS groups, respectively. Adjusted mean differences between groups were small, with confidence intervals including zero. The severe CTFS group demonstrated greater joint line convergence angle than the mild and moderate groups (both p < 0.001). Mean bearing thickness did not differ among groups (p = 0.428). CONCLUSIONS: Preoperative CTFS was not associated with worse outcomes in mobile-bearing UKA for AMOA with correctable deformity. Within the setting of correctable deformity and appropriate patient selection, preoperative CTFS was not associated with inferior 2-year outcomes. Longer-term survivorship and radiographic progression analyses are warranted. CI - (c) 2026. The Author(s). FAU - Kinoshita, Tomofumi AU - Kinoshita T AUID- ORCID: 0000-0001-6469-4652 AD - Department of Orthopaedic Surgery, Copenhagen University Hospital Hvidovre, Copenhagen, Denmark. kinoshita8u10@gmail.com. AD - Department of Orthopaedic Surgery, Ehime University Graduate School of Medicine, Toon, Japan. kinoshita8u10@gmail.com. FAU - Bunyoz, Kristine Ifigenia AU - Bunyoz KI AD - Department of Orthopaedic Surgery, Copenhagen University Hospital Hvidovre, Copenhagen, Denmark. FAU - Mortensen, Kristian R L AU - Mortensen KRL AD - Department of Orthopaedic Surgery, Copenhagen University Hospital Hvidovre, Copenhagen, Denmark. FAU - Bredgaard Jensen, Christian AU - Bredgaard Jensen C AD - Department of Orthopaedic Surgery, Copenhagen University Hospital Hvidovre, Copenhagen, Denmark. FAU - Price, Andrew AU - Price A AD - Nuffield Department of Orthopaedics, Rheumatology, and Musculoskeletal Sciences, University of Oxford, Oxford, United Kingdom. FAU - Gromov, Kirill AU - Gromov K AD - Department of Orthopaedic Surgery, Copenhagen University Hospital Hvidovre, Copenhagen, Denmark. AD - Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark. FAU - Troelsen, Anders AU - Troelsen A AD - Department of Orthopaedic Surgery, Copenhagen University Hospital Hvidovre, Copenhagen, Denmark. AD - Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark. LA - eng PT - Journal Article DEP - 20261029 PL - Germany TA - Arch Orthop Trauma Surg JT - Archives of orthopaedic and trauma surgery JID - 9011043 SB - IM MH - Humans MH - *Osteoarthritis, Knee/surgery/complications/diagnostic imaging MH - *Arthroplasty, Replacement, Knee/methods MH - Female MH - Retrospective Studies MH - Tibiofemoral Joint MH - Aged MH - Male MH - Treatment Outcome MH - Middle Aged OTO - NOTNLM OT - Coronal tibiofemoral subluxation OT - Mobile-bearing OT - Oxford knee score OT - Unicompartmental knee arthroplasty COIS- Declarations. Competing interests: Anders Troelsen reports research support from Zimmer Biomet and Pfizer Denmark, as well as consulting fees from both entities, and has served as an advisory board member for Zimmer Biomet and Pfizer Denmark. Andrew Price reports consulting fees from Zimmer Biomet and Medacta within the past 36 months. Kristian R. L. Mortensen received institutional support from Zimmer Biomet for the present work and travel support from Zimmer Biomet Denmark TPEC for EFORT 2023. All other financial and non-financial relationships, activities, or interests related to the present manuscript are declared none. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:16 PHST- 2026/01/14 00:00 [received] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:16 [entrez] AID - 10.1007/s00402-026-06530-x [pii] AID - 10.1007/s00402-026-06530-x [doi] PST - epublish SO - Arch Orthop Trauma Surg. 2026 Oct 29;146(1):387. doi: 10.1007/s00402-026-06530-x. PMID- 42955357 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1432-2048 (Electronic) IS - 0032-0935 (Linking) VI - 264 IP - 6 DP - 2026 Oct 28 TI - The mechanisms of indole-3-carboxylic acid-priming against Plectosphaerella cucumerina depend on the developmental stage of Arabidopsis. LID - 178 [pii] LID - 10.1007/s00425-026-05196-4 [doi] AB - Defense priming is age-dependent: mature leaves rely on starch-supported callose deposition, whereas young leaves redirect priming toward secondary metabolite accumulation due to limited carbon and energy availability. Plants display different defense mechanisms that change with age. Priming is an adaptive immune response that often uses different defense mechanisms compared with basal immunity. However, to the best of our knowledge, specific priming responses related to plant age have not been reported previously. Indole-3-carboxylic acid (I3CA)-induced priming against necrotrophs is mediated by PMR4-dependent callose accumulation in adult plants. Although I3CA-induced resistance (IR) is functional in seedlings, we concluded that priming in young plants is independent of callose accumulation, since it is functional in callose-deficient pmr4 mutants. The present study reveals that I3CA triggers strong changes before infection in adult plants at the protein level but displays a priming profile in seedlings that is more noticeable at the metabolomic level with relevant changes following infection. Amino acid, flavonoid, and glucosinolate biosynthesis are among the three major groups that follow a priming profile. Knockout plants deficient in the indole derivatives and, more specifically, in the indole-carboxylic acid (ICA) branch were impaired in I3CA-IR in seedlings. The atl31 and syp121 mutant seedlings, which are blocked in vesicular trafficking, are also impaired in I3CA-IR, revealing their relevance in priming at any developmental stage. These results indicate that I3CA priming is functionally independent of the age of the plant; however, the underlying mechanisms vary depending on the age of the plant. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Gamir, Jordi AU - Gamir J AD - Plant Immunity and Biochemistry Laboratory, Department of Biology, Biochemistry and Natural Sciences, Universitat Jaume I, 12071, Castellon, Spain. FAU - Flors, Victor AU - Flors V AD - Plant Immunity and Biochemistry Laboratory, Department of Biology, Biochemistry and Natural Sciences, Universitat Jaume I, 12071, Castellon, Spain. flors@uji.es. FAU - Sanchez-Bel, Paloma AU - Sanchez-Bel P AD - Plant Immunity and Biochemistry Laboratory, Department of Biology, Biochemistry and Natural Sciences, Universitat Jaume I, 12071, Castellon, Spain. FAU - Pastor, Victoria AU - Pastor V AUID- ORCID: 0000-0003-3750-4184 AD - Plant Immunity and Biochemistry Laboratory, Department of Biology, Biochemistry and Natural Sciences, Universitat Jaume I, 12071, Castellon, Spain. pastorm@uji.es. LA - eng GR - GV/2018/115/Conselleria de Cultura, Educacion y Ciencia, Generalitat Valenciana/ GR - UJI-A2019-05/Universitat Jaume I/ GR - CDEIGENT/2018/015/Generalitat Valenciana/ PT - Journal Article DEP - 20261028 PL - Germany TA - Planta JT - Planta JID - 1250576 RN - 0 (Indoles) RN - 59711R38B0 (indole-3-carboxylic acid) RN - 9064-51-1 (callose) RN - 0 (Arabidopsis Proteins) RN - 0 (Glucans) RN - 0 (Glucosinolates) RN - EC 2.4.1.- (PMR4 protein, Arabidopsis) RN - 0 (Flavonoids) RN - EC 2.4.1.- (Glucosyltransferases) SB - IM MH - *Indoles/metabolism/pharmacology MH - *Arabidopsis/microbiology/growth & development/immunology/metabolism/genetics MH - Arabidopsis Proteins/metabolism/genetics MH - *Plant Diseases/microbiology/immunology MH - Glucans/metabolism MH - Seedlings/microbiology/immunology/metabolism MH - Glucosinolates/metabolism MH - Plant Immunity MH - Plant Leaves/microbiology/metabolism MH - Flavonoids/metabolism MH - Gene Expression Regulation, Plant MH - Glucosyltransferases OTO - NOTNLM OT - Callose OT - Flavonoids OT - Induced resistance OT - Necrotrophs OT - Plant defense OT - Secondary metabolites COIS- Declarations. Conflict of interest: The authors declare no to have any conflict of interest to declare. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:16 PHST- 2026/01/28 00:00 [received] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:16 [entrez] AID - 10.1007/s00425-026-05196-4 [pii] AID - 10.1007/s00425-026-05196-4 [doi] PST - epublish SO - Planta. 2026 Oct 28;264(6):178. doi: 10.1007/s00425-026-05196-4. PMID- 42955355 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1437-9813 (Electronic) IS - 0179-0358 (Linking) VI - 42 IP - 1 DP - 2026 Oct 28 TI - Kangaroo mother care in neonates after surgery: a scoping review. LID - 464 [pii] LID - 10.1007/s00383-026-06696-w [doi] AB - AIM: To map the available evidence on the application of kangaroo mother care in neonates after surgery, and to summarize its intervention components, implementation methods, outcome measures, efficacy, and safety, thereby informing clinical practice and future research. METHODS: A systematic search was conducted in PubMed, CINAHL, CNKI, and Wanfang Database from inception to 7 November 2025. Empirical studies reporting the use of kangaroo mother care in postoperative neonatal populations were included, without restriction by study design. Data on intervention protocols, implementation methods, outcome measures, and safety-related events were extracted and collated. RESULTS: In total, 12 articles were selected from a pool of 4050 documents.They are six randomized controlled trials, two quasi-experimental studies, one case report, one quality improvement project, one cross-sectional study, and one qualitative literature review. The findings indicated that kangaroo mother care in neonates after surgery contributed to stabilized vital signs, reduced pain, earlier recovery of bowel function, and shorter hospital stays. Continuous monitoring of heart rate, oxygen saturation, and respiratory rate was identified as essential for safety during the intervention. CONCLUSIONS: Kangaroo mother care appears to offer several clinical benefits for neonates following surgery, although the evidence remains in an exploratory phase. Future research should prioritize standardizing intervention protocols, optimizing outcome measurement, and strengthening safety monitoring to support the structured implementation of this care practice in surgical neonatal units. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Wang, Haoyue AU - Wang H AUID- ORCID: 0009-0001-0944-5001 AD - Department of NICU, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua Univerity, No.168 Litang Road, Changping District, Beijing, China. wanghaoyue1211@163.com. FAU - Wang, Fei AU - Wang F AD - Department of NICU, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua Univerity, No.168 Litang Road, Changping District, Beijing, China. FAU - Peng, Xiaolan AU - Peng X AD - Department of NICU, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua Univerity, No.168 Litang Road, Changping District, Beijing, China. FAU - Xiao, Yanqing AU - Xiao Y AD - Department of NICU, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua Univerity, No.168 Litang Road, Changping District, Beijing, China. LA - eng PT - Journal Article PT - Review PT - Scoping Review DEP - 20261028 PL - Germany TA - Pediatr Surg Int JT - Pediatric surgery international JID - 8609169 SB - IM MH - Humans MH - *Kangaroo-Mother Care Method/methods MH - Infant, Newborn MH - *Postoperative Care/methods OTO - NOTNLM OT - After surgery OT - Kangaroo mother care OT - Neonate COIS- Declarations. Conflict of interest: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:16 PHST- 2026/09/19 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:16 [entrez] AID - 10.1007/s00383-026-06696-w [pii] AID - 10.1007/s00383-026-06696-w [doi] PST - epublish SO - Pediatr Surg Int. 2026 Oct 28;42(1):464. doi: 10.1007/s00383-026-06696-w. PMID- 42955350 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1438-8871 (Electronic) IS - 1438-8871 (Linking) VI - 28 DP - 2026 Oct 28 TI - Reporting Gaps and Challenges in Randomized Trials of Generative AI for Medical Use: Insights From a Systematic Review. PG - e94543 LID - 10.2196/94543 [doi] AB - BACKGROUND: Generative AI (GenAI) is being deployed rapidly in medicine, and evidence of its clinical safety and efficacy depends on transparent reporting of randomized controlled trials (RCTs). The CONSORT-AI (Consolidated Standards of Reporting Trials-AI) extension is a benchmark for AI trial reporting, but it was drafted for conventional AI, and its applicability to GenAI has not been examined. OBJECTIVE: This study aimed to assess item-level adherence to the CONSORT-AI among RCTs evaluating GenAI for medical purposes and to distinguish deficiencies attributable to nonadherence from those attributable to items that do not correspond to how GenAI interventions are specified or delivered. METHODS: PubMed, CENTRAL (Cochrane Central Register of Controlled Trials), and Embase were searched for peer-reviewed, English-language RCTs published from 2022 to April 3, 2026, supplemented by backward and forward citation searching, in which patients, health care professionals, or laypeople received a GenAI intervention for a medical purpose; non-GenAI, nonmedical, nonrandomized, and non-peer-reviewed reports were excluded. Two reviewers independently screened studies, extracted data, and rated CONSORT-AI items as reported, partially reported, not reported, or not applicable using signaling questions, with disagreements resolved by a third reviewer (Cohen kappa=0.81 and prevalence-adjusted, bias-adjusted kappa=0.84). Risk of bias was not assessed, as the target was reporting adherence. Adherence was the proportion of reports rated "reported" per item; item-level adherence was compared across 9 characteristics using Fisher exact and Freeman-Halton tests (2-sided alpha=.05). RESULTS: From 16,485 retrieved records, 74 reports from 73 RCTs (24,150 participants; median 100, IQR 50.0-247.0) were included. Large language models (LLMs) accounted for 98.6% (73/74) of interventions, with 60.8% (45/74) using GPT-family models. Item-level reporting adherence ranged from 0% (0/72) to 98.6% (73/74). High adherence occurred in general metadata and output specification items (range 59-73, 79.7%-98.6%). Conversely, reporting was poor for input and technical execution domains, input data eligibility (3/73, 4.1%), input acquisition/selection (9/74, 12.2%), algorithm versioning (17/74, 23.0%), trial setting integration (21/74, 28.4%), human-AI interaction (25/74, 33.8%), performance error (27/74, 36.5%), and open access/code (31/74, 41.9%). Input quality handling (Item 5[iii]) was completely unreported (0/72). Only 9 of 74 (12.2%) reports explicitly cited CONSORT-AI. While citing reports showed higher compliance for interaction and data sharing, major technical gaps persisted regardless of citation. CONCLUSIONS: This review provides the first empirical audit distinguishing general nonadherence from structural mismatches where deterministic CONSORT-AI items fail to capture GenAI mechanics. Differing from traditional AI evaluations, it reveals that key reproducibility drivers-system prompts, inference settings, and access modalities-are omitted from current standards. These empirical findings highlight critical reporting gaps in medical GenAI trials and identify domains for a tailored extension to current reporting standards, although the evidence is limited by the use of nonvalidated signaling questions. Establishing GenAI-specific trial guidelines will ensure that clinical decisions, regulatory approvals, and patient safety safeguards are grounded in reproducible and trustworthy evidence. CI - (c) Jae Eun Ko, Ji Woo Hwang, Sung Min Kim, Ji Yean Kwon. Originally published in the Journal of Medical Internet Research (https://www.jmir.org). FAU - Ko, Jae Eun AU - Ko JE AUID- ORCID: 0009-0003-6260-3077 AD - Department of Regulatory Science for Medical Deivce, Dongguk University, Pildong-ro 1gil 30, Jung-gu, Seoul, 04620, Republic of Korea, 82 319615616. FAU - Hwang, Ji Woo AU - Hwang JW AUID- ORCID: 0009-0001-8331-3488 AD - Department of Regulatory Science for Medical Deivce, Dongguk University, Pildong-ro 1gil 30, Jung-gu, Seoul, 04620, Republic of Korea, 82 319615616. FAU - Kim, Sung Min AU - Kim SM AUID- ORCID: 0000-0001-6457-989X AD - Department of Regulatory Science for Medical Deivce, Dongguk University, Pildong-ro 1gil 30, Jung-gu, Seoul, 04620, Republic of Korea, 82 319615616. AD - Department of Medical Device and Healthcare, Dongguk University, Seoul, Republic of Korea. AD - Department of Medical AI Engineering, College of Advanced Convergence Engineering, Dongguk University, Seoul, Republic of Korea. FAU - Kwon, Ji Yean AU - Kwon JY AUID- ORCID: 0000-0002-9685-4531 AD - Department of Regulatory Science for Medical Deivce, Dongguk University, Pildong-ro 1gil 30, Jung-gu, Seoul, 04620, Republic of Korea, 82 319615616. AD - Department of Medical Device and Healthcare, Dongguk University, Seoul, Republic of Korea. LA - eng PT - Journal Article PT - Review PT - Systematic Review DEP - 20261028 PL - Canada TA - J Med Internet Res JT - Journal of medical Internet research JID - 100959882 SB - IM MH - *Randomized Controlled Trials as Topic/standards MH - Generative Artificial Intelligence MH - Humans OTO - NOTNLM OT - AI OT - CONSORT-AI OT - Consolidated Standards of Reporting Trials-AI OT - generative artificial intelligence OT - large language models OT - randomized controlled trials OT - reporting adherence EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:16 PHST- 2026/03/03 00:00 [received] PHST- 2026/09/11 00:00 [revised] PHST- 2026/09/14 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:16 [entrez] AID - v28i1e94543 [pii] AID - 10.2196/94543 [doi] PST - epublish SO - J Med Internet Res. 2026 Oct 28;28:e94543. doi: 10.2196/94543. PMID- 42955345 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1432-0711 (Electronic) IS - 0932-0067 (Linking) VI - 313 IP - 1 DP - 2026 Aug 3 TI - Application of multi-technique integration in the prenatal diagnosis of mosaicism derived from an ICSI blastocyst: a diagnostic approach and clinical implications. LID - 301 [pii] LID - 10.1007/s00404-026-08547-4 [doi] AB - BACKGROUND: To investigate the prenatal diagnosis following intracytoplasmic sperm injection (ICSI) and blastocyst transfer. By integrating multiple cytogenetic and molecular techniques, we characterized a mosaic duplication of chromosome 20p, evaluated its pathogenicity and clinical significance, and provided evidence for prenatal genetic counseling. METHODS: Amniocentesis was performed in a pregnant woman identified as high-risk by non-invasive prenatal testing (NIPT), which suggested a 15q 26.3 deletion. Fetal amniotic fluid cells were analyzed using G-banding karyotyping, chromosomal microarray analysis (CMA), and fluorescence in situ hybridization (FISH). RESULTS: NIPT detected a 3.04 Mb deletion at 15q26.3.CMA showed a 20.51 Mb mosaic duplication involving 20p13p11.23, with an estimated mosaic proportion of approximately 50%, whereas G-banding karyotyping of amniotic fluid cells showed an unremarkable result. Mid-term FISH analysis performed in our hospital identified mosaicism for der(15)t(15;20)(qter;p13), indicating that a duplicated segment of 20p13 was translocated to the terminal region of chromosome 15. FISH analysis performed at an external hospital showed that 49% of cells carried an additional 20p13 signal. Peripheral blood karyotypes of both parents were unremarkable. CONCLUSION: This case illustrates how the integration of CMA, G-banding, and targeted FISH enables accurate characterization of complex chromosomal rearrangements in prenatal diagnosis. While these findings underscore the importance of thorough evaluation in ART-conceived pregnancies, they do not establish a causal relationship between assisted reproduction and the observed chromosomal abnormality. The high-level mosaic duplication of 20p13p11.23 is associated with significant pathogenic risk. Following comprehensive genetic counseling, the parents made an informed decision regarding pregnancy management. Couples with abnormal sperm parameters or repeated fertilization failure should be counseled about the potential risk of chromosomal mosaicism, and preimplantation genetic testing may be considered where indicated. CI - (c) 2026. The Author(s). FAU - Huang, Yun AU - Huang Y AD - Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China. 570428719@qq.com. AD - Guangdong Provincial Clinical Research Center for Laboratory Medicine, Guangzhou, China. 570428719@qq.com. FAU - Zhang, Shuping AU - Zhang S AD - Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China. AD - Guangdong Provincial Clinical Research Center for Laboratory Medicine, Guangzhou, China. FAU - Zou, Peng AU - Zou P AD - Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China. AD - Guangdong Provincial Clinical Research Center for Laboratory Medicine, Guangzhou, China. FAU - Li, Fang AU - Li F AD - Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China. AD - Guangdong Provincial Clinical Research Center for Laboratory Medicine, Guangzhou, China. FAU - Huang, Limin AU - Huang L AD - Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China. AD - Guangdong Provincial Clinical Research Center for Laboratory Medicine, Guangzhou, China. FAU - Li, Xiaofeng AU - Li X AD - Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China. 13530597138@163.com. AD - Guangdong Provincial Clinical Research Center for Laboratory Medicine, Guangzhou, China. 13530597138@163.com. FAU - Wang, He AU - Wang H AD - Department of Laboratory Medicine, Peking University Shenzhen Hospital, Shenzhen, China. herbert1977@163.com. AD - Guangdong Provincial Clinical Research Center for Laboratory Medicine, Guangzhou, China. herbert1977@163.com. LA - eng GR - 2023B110008/Guangdong Provincial Clinical Research Center for Laboratory Medicine/ GR - JCYJ20230807095202004; JCYJ20240813120016022；JCYJ20250604183845061/Shenzhen Science and Technology Innovation Program/ GR - 2023A1515010618/Applied Basic Research Foundation/ PT - Case Reports PT - Journal Article DEP - 20260803 PL - Germany TA - Arch Gynecol Obstet JT - Archives of gynecology and obstetrics JID - 8710213 SB - IM MH - Humans MH - Female MH - *Mosaicism/embryology MH - Pregnancy MH - *Sperm Injections, Intracytoplasmic MH - Amniocentesis MH - In Situ Hybridization, Fluorescence MH - Adult MH - Karyotyping MH - *Prenatal Diagnosis/methods MH - Chromosome Banding MH - Chromosomes, Human, Pair 15/genetics MH - Microarray Analysis OTO - NOTNLM OT - 20p mosaic duplication OT - Chromosome microarray analysis OT - Chromosome structural rearrangement OT - ICSI blastocyst OT - Prenatal diagnosis COIS- Declarations. Conflict of interest: The authors declare no competing interests. Ethical approval: Peking University Shenzhen Hospital Ethical Approval [Research] [2026] No. 286. Proofreading report: The current style in use is [National Standard of the People's Republic of China_GBT_7714-2005].The total number of bibliographic records in the current document is 25, with a total of 47 insertions at 34 positions (including repeated insertions).There are 0 bibliographic records with missing content in required fields. All bibliographic records have normal data. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:15 PHST- 2026/01/19 00:00 [received] PHST- 2026/07/21 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:15 [entrez] AID - 10.1007/s00404-026-08547-4 [pii] AID - 10.1007/s00404-026-08547-4 [doi] PST - epublish SO - Arch Gynecol Obstet. 2026 Aug 3;313(1):301. doi: 10.1007/s00404-026-08547-4. PMID- 42955327 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1654-9880 (Electronic) IS - 1654-9880 (Linking) VI - 19 IP - 1 DP - 2026 Dec TI - Humanitarian funding contraction and the risk of cost-related forgone care: a proposed financial-protection transition framework for internally displaced communities in Mogadishu, Somalia. PG - 2755139 LID - 10.1080/16549716.2026.2755139 [doi] AB - Somalia's humanitarian health response is contracting within a fragmented, aid-dependent system in which displaced households face direct payments and substantial access costs. National and humanitarian reports document major funding gaps and service reductions, but we identified no published study that directly estimates whether the recent funding contraction has increased household spending, delays in care, treatment interruption or cost-related forgone care among internally displaced communities in Mogadishu. This Current Debate is a purposive policy analysis based on selected peer-reviewed studies, Somali policy documents, humanitarian reports and international guidance; it is not an empirical study or a systematic review. We distinguish financial hardship among households that obtain care from cost-related financial exclusion, a broader construct encompassing delayed, incomplete or completely forgone needed care due to unaffordable direct or access costs. We propose a testable transition framework for both planned service reductions and abrupt withdrawal, linking service-gap assessment and essential-service prioritisation to funded protection from charges, time-limited bridge arrangements, appropriately governed purchasing, medicine and transport support, adaptive social protection and community accountability. A minimum viable monitoring set should combine indicators of service continuity, prescription fill, referral completion and unauthorised charges with household-level distress financing and should report cost-related delayed, incomplete and completely forgone care separately. The proposed pathways and interventions are evidence-informed hypotheses and policy options requiring local co-design, costing, feasibility assessment and prospective evaluation; they should not be interpreted as evidence that humanitarian funding contraction has already caused cost-related forgone care in Mogadishu. FAU - Siyad, Ahmed Omar AU - Siyad AO AUID- ORCID: 0009-0000-0198-664X AD - Department of Intensive Care Medicine, Dr. Sumait Hospital, SIMAD University, Mogadishu, Somalia. FAU - Hassan, Abdukadir Mohamed AU - Hassan AM AUID- ORCID: 0009-0006-8096-9736 AD - Department of Intensive Care Medicine, Dr. Sumait Hospital, SIMAD University, Mogadishu, Somalia. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Glob Health Action JT - Global health action JID - 101496665 SB - IM MH - Humans MH - Somalia MH - *Altruism MH - *Relief Work/economics/organization & administration MH - *Refugees MH - *Health Services Accessibility/economics OAB - Main findings: National evidence documents Somalia's humanitarian health-service contraction, but no direct evidence establishes its effect on forgone care among displaced Mogadishu households.Added knowledge: This Current Debate proposes a testable framework linking planned and abrupt funding withdrawal to separate hardship and delayed, incomplete or completely forgone care indicators.Global health impact for policy and action: Funding reductions demand financed service continuity, explicit clinical priorities, accountable purchasing and monitoring that detects both hardship and exclusion. OABL- eng OTO - NOTNLM OT - Universal health coverage OT - adaptive social protection OT - service continuity OT - strategic purchasing OT - unmet healthcare need EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:15 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:15 [entrez] AID - 10.1080/16549716.2026.2755139 [doi] PST - ppublish SO - Glob Health Action. 2026 Dec;19(1):2755139. doi: 10.1080/16549716.2026.2755139. Epub 2026 Oct 29. PMID- 42955323 OWN - NLM STAT- Publisher LR - 20261029 IS - 1432-1238 (Electronic) IS - 0342-4642 (Linking) DP - 2026 Oct 29 TI - Immunological signatures of sepsis in abdominal solid organ transplant recipients: A multilayer and multicentric prospective cohort study. LID - 10.1007/s00134-026-08612-1 [doi] AB - BACKGROUND: Sepsis is defined as life-threatening organ dysfunction resulting from a dysregulated host response to infection. Patients with prior abdominal solid organ transplantation (SOT) are highly susceptible to infection due to chronic immunosuppression, yet exhibit improved survival once sepsis occurs. The underlying mechanisms remain poorly understood. METHODS: We conducted a post hoc analysis within a prospective multicenter cohort of septic patients (SepsisDataNet.NRW; n = 538). Clinical outcomes were assessed using Kaplan-Meier analysis and multivariable Cox regression. Immune profiling included serum cytokines, flow cytometric immunophenotyping, plasma proteomics, and intracellular signaling using proximity ligation assays (PLA) in peripheral blood mononuclear cells (PBMCs). To reduce clinical confounding, complementary exploratory analyses were performed in a non-septic kidney transplant cohort (e:KID). RESULTS: SOT patients demonstrated improved 30-day survival compared with non-SOT patients (84% vs. 66%; adjusted HR 0.49, 95% CI 0.26-0.95). Cytokine analysis revealed lower IL-6 and TNF-alpha levels alongside higher IL-18 concentrations, indicating an altered inflammatory profile. Flow cytometry showed reductions in adaptive immune cells but relatively preserved innate immune function, reflected by higher monocytic HLA-DR expression. Proteomics identified enrichment of innate immune, complement, and lipid metabolic response networks. PLA-based cellular analyses revealed increased GLUT1 expression and enhanced TFAM/TFB2M interaction in SOT patients, indicating a distinct immunometabolic marker profile. In e:KID patients paired serum metabolomics demonstrated higher glucose and lower lactate concentrations 12 months after transplantation, accompanied by broader alterations in lipid and branched-chain amino acid profiles. CONCLUSION: In patients with sepsis, abdominal SOT is associated with significant better survival than in non-SOT patients. Septic abdominal SOT patients display a distinct immune phenotype characterized by attenuated systemic inflammation, relative preservation of selected innate immune readouts, and alterations in immunometabolic markers. CI - (c) 2026. The Author(s). FAU - Witowski, Andrea AU - Witowski A AUID- ORCID: 0009-0000-6522-832X AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Bochum, Germany. andrea.witowski@ruhr-uni-bochum.de. AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Center for Perioperative Precision Medicine, Bochum, Germany. andrea.witowski@ruhr-uni-bochum.de. FAU - Bracht, Thilo AU - Bracht T AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Center for Clinical Proteomic and Metabolomic, Bochum, Germany. AD - Ruhr University Bochum, Medical Faculty, Medizinisches Proteom-Center, Bochum, Germany. FAU - Kroger, Lena AU - Kroger L AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Center for Perioperative Precision Medicine, Bochum, Germany. FAU - Rump, Katharina AU - Rump K AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Center for Perioperative Precision Medicine, Bochum, Germany. FAU - Nowak, Hartmuth AU - Nowak H AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Bochum, Germany. AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Center for Artificial Intelligence, Medical Informatics and Data Science, Bochum, Germany. FAU - Bergmann, Lars AU - Bergmann L AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Bochum, Germany. FAU - Unterberg, Matthias AU - Unterberg M AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Bochum, Germany. FAU - Rahmel, Tim AU - Rahmel T AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Bochum, Germany. FAU - Westhus, Britta AU - Westhus B AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Bochum, Germany. FAU - Wolf, Alexander AU - Wolf A AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Bochum, Germany. FAU - Thon, Patrick AU - Thon P AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Center for Perioperative Precision Medicine, Bochum, Germany. FAU - Ziehe, Dominik AU - Ziehe D AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Center for Perioperative Precision Medicine, Bochum, Germany. FAU - Putensen, Christian AU - Putensen C AD - Klinik fur Anasthesiologie und operative Intensivmedizin, Universitatsklinikum Bonn, Bonn, Germany. FAU - Ehrentraut, Stefan AU - Ehrentraut S AD - Klinik fur Anasthesiologie und operative Intensivmedizin, Universitatsklinikum Bonn, Bonn, Germany. FAU - Zarbock, Alexander AU - Zarbock A AD - Department of Anesthesiology, Intensive Care and Pain Medicine, University Hospital Munster, Munster, Germany. FAU - Wappler, Frank AU - Wappler F AD - Department of Anaesthesiology and Critical Care Medicine, Merheim Medical Center, Witten/Herdecke University, Cologne, Germany. FAU - Defosse, Jerome AU - Defosse J AD - Department of Anaesthesiology and Critical Care Medicine, Merheim Medical Center, Witten/Herdecke University, Cologne, Germany. FAU - Henzler, Dietrich AU - Henzler D AD - Klinikum Herford, Department of Anesthesiology, Surgical Intensive Care, Emergency and Pain Medicine, Ruhr University Bochum, Herford, Germany. FAU - Sitek, Barbara AU - Sitek B AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Center for Clinical Proteomic and Metabolomic, Bochum, Germany. AD - Ruhr University Bochum, Medical Faculty, Medizinisches Proteom-Center, Bochum, Germany. FAU - Schnitzbauer, Andreas AU - Schnitzbauer A AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Surgery, Bochum, Germany. FAU - Stervbo, Ulrik AU - Stervbo U AD - Centrum fur Translationale Medizin, Medizinische Klinik I, Marien Hospital Herne, Universitatsklinikum der Ruhr-Universitat Bochum, Herne, Deutschland. FAU - Rosiewicz, Kamil AU - Rosiewicz K AD - Centrum fur Translationale Medizin, Medizinische Klinik I, Marien Hospital Herne, Universitatsklinikum der Ruhr-Universitat Bochum, Herne, Deutschland. FAU - Hugo, Christian AU - Hugo C AD - Universitatsklinikum Carl Gustav Carus Dresden, Medizinische Klinik und Poliklinik III, Nephrologie, Dresden, Germany. FAU - Neumann, Sindy AU - Neumann S AD - numares AG, Regensburg, Germany. FAU - Pagel, Philipp AU - Pagel P AD - numares AG, Regensburg, Germany. FAU - Pascher, Andreas AU - Pascher A AD - Department of General, Visceral and Transplant Surgery, University Hospital Munster, Munster, Germany. FAU - Babel, Nina AU - Babel N AD - Centrum fur Translationale Medizin, Medizinische Klinik I, Marien Hospital Herne, Universitatsklinikum der Ruhr-Universitat Bochum, Herne, Deutschland. FAU - Adamzik, Michael AU - Adamzik M AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Bochum, Germany. FAU - Dyck, Birte AU - Dyck B AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Center for Perioperative Precision Medicine, Bochum, Germany. FAU - Koos, Bjorn AU - Koos B AD - Ruhr University Bochum, Knappschaft Kliniken University Hospital Bochum, Department of Anesthesiology, Intensive Care Medicine and Pain Therapy, Center for Perioperative Precision Medicine, Bochum, Germany. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Intensive Care Med JT - Intensive care medicine JID - 7704851 SB - IM OTO - NOTNLM OT - Critical care OT - Immunometabolism OT - Innate immunity OT - Sepsis OT - SepsisDataNet.NRW OT - Solid organ transplantation COIS- Declarations. Conflict of interest: The authors declare no competing interests. Ethics approval and consent to participate: The study was approved by the Ethics Committee of the Medical Faculty of Ruhr University Bochum (For SepsisDataNet.NRW: No. 18-6606-BR and No. 22-7477; for e:KID: 18-6304, EA1/112/17). Written informed consent was obtained from all participants or their legal representatives. The study was conducted in accordance with the Declaration of Helsinki and local regulatory requirements. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:15 PHST- 2026/05/28 00:00 [received] PHST- 2026/08/28 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:15 [entrez] AID - 10.1007/s00134-026-08612-1 [pii] AID - 10.1007/s00134-026-08612-1 [doi] PST - aheadofprint SO - Intensive Care Med. 2026 Oct 29. doi: 10.1007/s00134-026-08612-1. PMID- 42955320 OWN - NLM STAT- Publisher LR - 20261029 IS - 1432-1238 (Electronic) IS - 0342-4642 (Linking) DP - 2026 Oct 29 TI - Ventilator-associated pneumonia after traumatic brain injury: a timing-sensitive ecological framework of neuroimmune-pulmonary vulnerability. LID - 10.1007/s00134-026-08606-z [doi] AB - BACKGROUND: Ventilator-associated pneumonia (VAP) is frequent after traumatic brain injury (TBI), but its biological meaning and outcome impact remain incompletely defined. Traditional models emphasise mechanical ventilation, aspiration, colonisation, endotracheal tube biofilm, and hospital pathogens. These mechanisms remain essential, but they do not fully explain the early clustering, heterogeneous susceptibility, changing bacteriology, and variable mortality effect observed after TBI. MAIN TEXT: Severe brain injury creates an early vulnerable host state through impaired consciousness and airway protection, neuroimmune dysregulation, autonomic and endocrine activation, altered pulmonary immune surveillance, and, in many patients, extracranial trauma, shock, transfusion, or thoracic injury. These processes interact with ventilation, antibiotic pressure, and rapid respiratory microbiome disruption. Early VAP may reflect abrupt loss of control after injury and intubation, whereas late VAP may reflect persistent or secondary failure driven by biofilm maturation, antibiotic selection, multidrug-resistant pathogens, and intensive care unit (ICU)-acquired immune dysfunction. Clinically, VAP consistently prolongs ventilation, ICU stay, antibiotic exposure, and resource use, while its independent effect on mortality is phenotype- and context-dependent. CONCLUSIONS: This model proposes a timing-sensitive ecological framework in which established mechanisms may converge early and with particular clinical intensity after severe TBI, creating a vulnerable host-microbe state that may lower the threshold for clinically apparent infection. Future studies should distinguish isolated TBI from polytrauma, compare TBI with other acute brain injuries and non-neurological ventilated populations, and incorporate dynamic immune, microbial, and trajectory-based outcomes. HIGHLIGHTS: VAP after TBI should be viewed as a timing-sensitive interaction between ventilator exposure and early host vulnerability, not as a distinct disease entity or as a pneumonia pathway unique to traumatic brain injury. The TBI-focused contribution lies in the early convergence of otherwise non-unique mechanisms: impaired airway control, aspiration risk, neuroimmune-endocrine activation, treatment exposure, dysbiosis, and extracranial injury burden, rather than in any single TBI-specific mechanism. Isolated TBI and TBI with polytrauma are biologically different phenotypes and should be separated in future studies. Early and late VAP may represent different phases of loss of pulmonary host-microbe containment; this distinction is probabilistic rather than TBI-specific in itself and requires validation in TBI-stratified cohorts. Mortality remains clinically relevant but context-dependent; VAP more consistently modifies recovery trajectory, ventilator dependence, ICU stay, antibiotic exposure, and possibly neurological recovery. CI - (c) 2026. The Author(s). FAU - Martin-Loeches, Ignacio AU - Martin-Loeches I AD - Department of Intensive Care Medicine, Multidisciplinary Intensive Care Research Organization (MICRO), St James' Hospital, Dublin, Ireland. AD - Trinity College Dublin, Dublin, Ireland. FAU - Povoa, Pedro AU - Povoa P AD - NOVA Medical School, CHRC, NOVA University of Lisbon, Lisbon, Portugal. AD - Center for Clinical Epidemiology and Research Unit of Clinical Epidemiology, OUH Odense University Hospital, Odense, Denmark. AD - D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Brazil. AD - Department of Critical Care Medicine, Hospital de Sao Francisco Xavier, ULS Lisboa Ocidental, Lisbon, Portugal. FAU - Robba, Chiara AU - Robba C AD - IRCCS Azienda Ospedaliera Metropolitana, Genoa, Italy. AD - Dipartimento di Scienze Chirurgiche Diagnostiche ed Integrate, University of Genoa, Genoa, Italy. FAU - Bandera, Alessandra AU - Bandera A AD - Infectious Diseases Unit, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy. AD - Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy. FAU - Guglielmi, Angelo AU - Guglielmi A AD - University of Pavia, PhD in Experimental Medicine, Pavia, Italy. AD - Anesthesia and Intensive Care Department 1, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy. FAU - Citerio, Giuseppe AU - Citerio G AUID- ORCID: 0000-0002-5374-3161 AD - School of Medicine and Surgery, University of Milano-Bicocca, Milan, Italy. giuseppe.citerio@unimib.it. AD - NeuroIntensive Care Unit, Neuroscience Department, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy. giuseppe.citerio@unimib.it. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - United States TA - Intensive Care Med JT - Intensive care medicine JID - 7704851 SB - IM OTO - NOTNLM OT - Ventilator-associated pneumonia OT - aspiration OT - compartmentalised immunity OT - dysbiosis OT - neuroimmune-endocrine dysfunction OT - polytrauma OT - recovery trajectory OT - respiratory microbiome OT - traumatic brain injury OT - ventilator-associated tracheobronchitis COIS- Declarations. Conflict of interest: CR is a Deputy Editor of Intensive Care Medicine. She has not taken part in the review or selection process of this article. PP is a Senior Associate Editor for Intensive Care Medicine. He has not taken part in the review or selection process of this article. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Use of artificial intelligence tools: Artificial intelligence-assisted tools, including ChatGPT, were used solely to support graphical development, visual refinement, and language editing. These tools were not used to generate scientific interpretation, select references, draw conclusions, or replace author judgement. All scientific content, figure content, and final text were critically reviewed, edited, and approved by the authors, who take full responsibility for the accuracy and integrity of the work. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:15 PHST- 2026/04/30 00:00 [received] PHST- 2026/08/20 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:15 [entrez] AID - 10.1007/s00134-026-08606-z [pii] AID - 10.1007/s00134-026-08606-z [doi] PST - aheadofprint SO - Intensive Care Med. 2026 Oct 29. doi: 10.1007/s00134-026-08606-z. PMID- 42955318 OWN - NLM STAT- Publisher LR - 20261029 IS - 1433-0458 (Electronic) IS - 0017-6192 (Linking) DP - 2026 Oct 28 TI - [Radiotherapy of head and neck cancer : Highlights from the 2026 ASCO Annual Meeting]. LID - 10.1007/s00106-026-01817-2 [doi] AB - At the 2026 American Society of Clinical Oncology (ASCO) Annual Meeting, several studies focused on radiotherapy of head and neck squamous cell carcinoma (HNSCC). The phase III AREST trial showed that adjuvant radiotherapy for early oral cavity cancer with intermediate-risk features can reduce the risk of locoregional recurrence. A post hoc analysis of the JCOG1008 trial found no increased locoregional recurrence rate even when adjuvant chemoradiotherapy was initiated later than recommended. Combined radioimmunotherapy (RIT) was also a major topic this year: in a subgroup analysis of the NIVOPOSTOP trial, the disease-free survival (DFS) benefit of nivolumab was maintained in patients with extensive neck dissection, which weakens the frequently highlighted mechanistic relevance of regional lymph nodes for the immune response. In the treatment of human papillomavirus (HPV)-positive oropharyngeal cancer, results for RIT were conflicting: the phase II CCTG HN.9 trial evaluating radiotherapy combined with durvalumab failed to meet its efficacy endpoint, whereas the phase II KEYCHAIN trial of definitive RIT with pembrolizumab demonstrated a significant improvement in progression-free survival (PFS) compared with cisplatin-based chemoradiotherapy. In addition, a subgroup analysis of the combined positive score (CPS) >/= 10 population of the KEYNOTE-412 trial underscored the importance of biomarker-based patient selection when combining radiotherapy and immunotherapy. CI - (c) 2026. The Author(s), under exclusive licence to Springer Medizin Verlag GmbH, ein Teil von Springer Nature. FAU - Schnellhardt, Soren AU - Schnellhardt S AUID- ORCID: 0000-0003-0418-4401 AD - Klinik fur Strahlentherapie und Radioonkologie, Universitatsklinikum des Saarlandes, Kirrberger Strasse, Gebaude 6.5, 66421, Homburg/Saar, Deutschland. soeren.schnellhardt@uks.eu. FAU - Tribius, Silke AU - Tribius S AD - Hermann-Holthusen-Institute for Radiation Oncology, Asklepios Tumor Zentrum Hamburg, Asklepios Hospital St. Georg, Hamburg, Deutschland. FAU - Linxweiler, Maximilian AU - Linxweiler M AD - Department of Otorhinolaryngology, Head and Neck Surgery, Saarland University Medical Center, Homburg, Deutschland. FAU - Suss, Christoph AU - Suss C AD - Department of Radiotherapy, Hospital of the University of Regensburg, Regensburg, Deutschland. FAU - Hecht, Markus AU - Hecht M AD - Klinik fur Strahlentherapie und Radioonkologie, Universitatsklinikum des Saarlandes, Kirrberger Strasse, Gebaude 6.5, 66421, Homburg/Saar, Deutschland. LA - ger PT - English Abstract PT - Journal Article PT - Review TT - Strahlentherapie von Kopf-Hals-Tumoren : Highlights der ASCO-Jahrestagung 2026. DEP - 20261028 PL - Germany TA - HNO JT - HNO JID - 2985099R SB - IM OTO - NOTNLM OT - Head and neck squamous cell carcinoma OT - Immune checkpoint inhibitors OT - Immunotherapy OT - Neck dissection OT - Oropharyngeal cancers COIS- Einhaltung ethischer Richtlinien. Interessenkonflikt: S. Schnellhardt: Merck Healthcare Germany (Vortrage, Reisemittel), MSD (Reisemittel). S. Tribius: Merck Serono (Advisory Boards, Vortragshonorar, Reisemittel); AstraZeneca (Vortragshonorar), BMS (Advisory Boards), Sanofi Genzyme (Advisory Board, Vortragshonorar, Reisemittel), MedKom (Vortragshonorar); OnkoWissen (Vortragshonorar); Asklepios ProResearch (Forschungsfinanzierung). M. Linxweiler.: MSD (Vortrage, Reisemittel); KARL STORZ (Advisory Board, Vortrage, Reisemittel, Forschungsfinanzierung), Merck Healthcare Germany (Vortrage), AstraZeneca (Vortrage, Reisemittel), BMS (Vortrage). und M. Hecht: Merck Serono (Advisory Boards, Vortrage, Reisemittel, Forschungsfinanzierung); MSD (Advisory Boards, Vortrage, Reisemittel, Forschungsfinanzierung); AstraZeneca (Advisory Boards, Vortrage, Reisemittel, Forschungsfinanzierung); Novartis (Forschungsfinanzierung); BMS (Advisory Boards, Vortrage, Reisemittel, Forschungsfinanzierung); Sanofi Genzyme (Advisory Boards, Forschungsfinanzierung). C. Suss gibt an, dass kein Interessenkonflikt besteht. Fur diesen Beitrag wurden von den Autor/-innen keine Studien an Menschen oder Tieren durchgefuhrt. Fur die aufgefuhrten Studien gelten die jeweils dort angegebenen ethischen Richtlinien. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:14 PHST- 2026/08/09 00:00 [received] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:14 [entrez] AID - 10.1007/s00106-026-01817-2 [pii] AID - 10.1007/s00106-026-01817-2 [doi] PST - aheadofprint SO - HNO. 2026 Oct 28. doi: 10.1007/s00106-026-01817-2. PMID- 42955316 OWN - NLM STAT- Publisher LR - 20261029 IS - 1433-0458 (Electronic) IS - 0017-6192 (Linking) DP - 2026 Oct 28 TI - [Neurostimulation in the treatment of obstructive sleep apnea]. LID - 10.1007/s00106-026-01808-3 [doi] AB - One of the most common sleep-related breathing disorders is obstructive sleep apnea (OSA), which is associated with increased cardiovascular morbidity. Pathophysiologically, OSA is caused by collapse of the upper airway due to a sleep-induced reduction in muscle tone. The gold standard treatment is continuous positive airway pressure (CPAP) therapy; however, due to limited adherence , it is insufficient as the sole treatment for some patients. Hypoglossal nerve stimulation (HGNS) as an alternative treatment aims to activate the protrusion muscles of the tongue, thereby stabilizing the airway. Clinical studies show a significant reduction in the apnea-hypopnea index (AHI) and an improvement in daytime symptoms even in long-term data. A prerequisite for therapeutic success is careful and thorough patient selection. CI - (c) 2026. The Author(s). FAU - Burian, Nora-Maria AU - Burian NM AD - Universitatsklinik fur Hals-Nasen-Ohrenheilkunde, Universitatsklinik Innsbruck, Anichstrasse 35, 6020, Innsbruck, Osterreich. nora-maria.burian@tirol-kliniken.at. FAU - Bender, Birte AU - Bender B AD - Universitatsklinik fur Hals-Nasen-Ohrenheilkunde, Universitatsklinik Innsbruck, Anichstrasse 35, 6020, Innsbruck, Osterreich. FAU - Hofauer, Benedikt AU - Hofauer B AD - Universitatsklinik fur Hals-Nasen-Ohrenheilkunde, Universitatsklinik Innsbruck, Anichstrasse 35, 6020, Innsbruck, Osterreich. LA - ger PT - English Abstract PT - Journal Article TT - Neurostimulationsverfahren in der Behandlung der obstruktiven Schlafapnoe. DEP - 20261028 PL - Germany TA - HNO JT - HNO JID - 2985099R SB - IM OTO - NOTNLM OT - Airway obstruction OT - Body mass index OT - Continuous positive airway pressure OT - Patient selection OT - Sleep-disordered breathing COIS- Einhaltung ethischer Richtlinien. Interessenkonflikt: Gemass den Richtlinien des Springer Medizin Verlags werden Autorinnen und Autoren sowie die Wissenschaftliche Leitung im Rahmen der Manuskripterstellung und Manuskriptfreigabe aufgefordert, eine vollstandige Erklarung zu ihren finanziellen und nichtfinanziellen Interessen abzugeben. Autorinnen und Autoren: N.-M. Burian: A. Finanzielle Interessen: N.-M. Burian gibt an, dass kein finanzieller Interessenkonflikt besteht. - B. Nichtfinanzielle Interessen: Facharztin an der Universitatsklinik fur Hals‑, Nasen- und Ohrenheilkunde | Mitgliedschaften: Deutsche Gesellschaft fur Hals-Nasen-Ohren-Heilkunde und Kopf-Hals-Chirurgie, Osterreichische Gesellschaft fur Hals-Nasen-Ohren-Heilkunde und Kopf-Hals-Chirurgie, Osterreichische Gesellschaft fur Schlafmedizin und Schlafforschung B. Bender: A. Finanzielle Interessen: Vortragshonorar (Inspire Medical Systems), Reise/Ubernachtungskostenerstattung (MED-EL Elektromedizinische Gerate GmbH, Nyxoah SA). - Eli Lilly Medical - Advisory Board Tirzepatid. - B. Nichtfinanzielle Interessen: Angestellte Oberarztin, Univ. HNO-Klinik Innsbruck, Tirol Kliniken | Mitgliedschaften: Deutsche Gesellschaft fur Hals-Nasen-Ohren-Heilkunde und Kopf-Hals-Chirurgie, Osterreichische Gesellschaft fur Hals-Nasen-Ohren-Heilkunde und Kopf-Hals-Chirurgie, hier Vorsitzende ARGE Schlafmedizin, Osterreichische Gesellschaft fur Schlafmedizin und Schlafforschung, hier Fachbeirat HNO. B. Hofauer: A. Finanzielle Interessen: AstraZeneca, MED-EL. - MED-EL, Inspire Medical Systems, GSK, MSD, Canon Medical. - Inspire Medical Systems. - B. Nichtfinanzielle Interessen: Klinikdirektor der Universitatsklinik Hals‑, Nasen- und Ohrenheilkunde, Universitatsprofessor fur Hals‑, Nasen- und Ohrenheilkunde | Vorstandsmitglied der Osterreichischen HNO-Gesellschaft. Wissenschaftliche Leitung: Die vollstandige Erklarung zum Interessenkonflikt der Wissenschaftlichen Leitung finden Sie am Kurs der zertifizierten Fortbildung auf www.springermedizin.de/cme . Der Verlag: erklart, dass fur die Publikation dieser CME-Fortbildung keine Sponsorengelder an den Verlag fliessen. Fur diesen Beitrag wurden von den Autor/-innen keine Studien an Menschen oder Tieren durchgefuhrt. Fur die aufgefuhrten Studien gelten die jeweils dort angegebenen ethischen Richtlinien. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:14 PHST- 2026/06/16 00:00 [received] PHST- 2026/07/10 00:00 [accepted] PHST- 2026/07/10 00:00 [revised] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:14 [entrez] AID - 10.1007/s00106-026-01808-3 [pii] AID - 10.1007/s00106-026-01808-3 [doi] PST - aheadofprint SO - HNO. 2026 Oct 28. doi: 10.1007/s00106-026-01808-3. PMID- 42955313 OWN - NLM STAT- Publisher LR - 20261029 IS - 1432-1920 (Electronic) IS - 0028-3940 (Linking) DP - 2026 Oct 29 TI - Serial MRI of spontaneous spinal epidural hematoma in infancy managed conservatively: a case report. LID - 10.1007/s00234-026-04241-z [doi] AB - We present a case report on the conservative treatment of spontaneous spinal epidural hematomas (SSEHs) in infancy, a very rare condition. Physical examinations and serial MRIs were conducted for SSEHs located between C7 and T4. About 4 months after the onset of the first symptoms, normal muscle strength was observed in the lower limbs of the patient who had experienced bilateral lower limb paralysis, and MRI results indicated recovery in the affected nerve area. This single case suggests that, in carefully selected infants who show early signs of spontaneous neurological improvement, conservative treatment may be considered as an option for managing SSEHs; however, given the limitations of a single case report, further studies are needed before this approach can be more broadly recommended. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Kim, Young-Hoon AU - Kim YH AUID- ORCID: 0000-0003-1237-4600 AD - Department of Orthopaedic Surgery, Seoul St. Mary's Hospital, Collegeof Medicine, The Catholic University of Korea, Seoul, Republic of Korea. FAU - Seo, Dong In AU - Seo DI AD - Department of Orthopaedic Surgery, Incheon St. Mary's Hospital,College of Medicine, The Catholic University of Korea, Incheon, Republic of Korea. FAU - Bang, Chungwon AU - Bang C AUID- ORCID: 0000-0002-6138-1527 AD - Department of Orthopaedic Surgery, Incheon St. Mary's Hospital,College of Medicine, The Catholic University of Korea, Incheon, Republic of Korea. freeboy3645@gmail.com. LA - eng PT - Journal Article DEP - 20261029 PL - Germany TA - Neuroradiology JT - Neuroradiology JID - 1302751 SB - IM OTO - NOTNLM OT - Conservative treatment OT - Infant OT - Magnetic resonance imaging OT - Paraplegia OT - Pediatric spine OT - Spontaneous spinal epidural hematoma COIS- Declarations. Ethics and Consent to Participate: The requirement for ethical approval was waived by the Institutional Review Board of The Catholic University of Korea because it is a case report. IRB No. is 2026-0950-0001. The need for informed consent was waived by the ethics committee because of the retrospective nature of the study. Consent for publication: Written informed consent for publication of clinical details, images, and videos was obtainedfrom the patient's parents. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:14 PHST- 2026/01/07 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:14 [entrez] AID - 10.1007/s00234-026-04241-z [pii] AID - 10.1007/s00234-026-04241-z [doi] PST - aheadofprint SO - Neuroradiology. 2026 Oct 29. doi: 10.1007/s00234-026-04241-z. PMID- 42955290 OWN - NLM STAT- Publisher LR - 20261029 IS - 1618-2650 (Electronic) IS - 1618-2642 (Linking) DP - 2026 Oct 28 TI - Development of a multi-platform MS approach for the determination of organic micropollutants in terrestrial invertebrates. LID - 10.1007/s00216-026-06882-8 [doi] AB - Terrestrial invertebrates occupy key ecosystem functions and accumulate organic micropollutants (OMPs) from agricultural, urban, and industrial sources, yet existing biomonitoring methods typically target narrow, class-specific chemical spaces. Here, four sample preparation methods, two based on bead beating (BB) and two on accelerated solvent extraction (ASE), were developed and compared through a smart validation scheme for gas and liquid chromatography coupled to high-resolution mass spectrometry (GC-HRMS, LC-HRMS). The two best-performing methods, BB-GC and BB-LC, were validated across three platforms: GC-HRMS, LC-HRMS, and GC tandem mass spectrometry (GC-MS/MS). A chemometric approach selected a validation set spanning plant protection products, pharmaceuticals, per- and polyfluoroalkyl substances, polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), polychlorinated naphthalenes, and organochlorine pesticides, spanning the chemical space of in-house databases of more than 2800 unique compounds. In total, 303 LC-HRMS, 179 GC-HRMS, and 82 GC-MS/MS analytes were validated. Under the strict criteria of mean recovery of 70-120% and repeatability of /= 60 was associated with recurrent neoplasia (p < 0.0001). Progression to HGUC was observed only among patients with an EpiScore >/= 60, although this finding should be interpreted cautiously because of the limited number of progression events and the absence of events in the EpiScore < 60 group. In conclusion, urinary methylation analysis may identify a subgroup of patients with LGUC associated with recurrence and grade progression to HGUC. These findings are exploratory and require prospective validation in larger and more heterogeneous cohorts. CI - (c) 2026. The Author(s). FAU - Pierconti, Francesco AU - Pierconti F AUID- ORCID: 0000-0001-9111-0165 AD - Division of Anatomic Pathology and Histology, Agostino Gemelli School of Medicine, Catholic University of the Sacred Heart, Largo Francesco Vito 1, Rome, 00168, Italy. francesco.pierconti@unicatt.it. FAU - Fiorentino, Vincenzo AU - Fiorentino V AD - Department of Human Pathology in Adult and Developmental Age "Gaetano Barresi", Pathology Section, University of Messina, Messina, Italy. FAU - Larocca, Luigi Maria AU - Larocca LM AD - Unicamillus, International Medical University in Rome, Roma, Italy. FAU - Merenda, Elisabetta AU - Merenda E AD - Division of Anatomic Pathology and Histology, Agostino Gemelli School of Medicine, Catholic University of the Sacred Heart, Largo Francesco Vito 1, Rome, 00168, Italy. FAU - Zhang, Qianqian AU - Zhang Q AD - Division of Anatomic Pathology and Histology, Agostino Gemelli School of Medicine, Catholic University of the Sacred Heart, Largo Francesco Vito 1, Rome, 00168, Italy. FAU - Pizzimenti, Cristina AU - Pizzimenti C AD - Azienda Ospedaliera Papardo, Contrada Papardo, Messina, 98158, Italy. FAU - Pepe, Ludovica AU - Pepe L AD - Department of Human Pathology in Adult and Developmental Age "Gaetano Barresi", Pathology Section, University of Messina, Messina, Italy. FAU - Rossi, Esther Diana AU - Rossi ED AD - Division of Anatomic Pathology and Histology, Agostino Gemelli School of Medicine, Catholic University of the Sacred Heart, Largo Francesco Vito 1, Rome, 00168, Italy. FAU - Granitto, Alessia AU - Granitto A AD - Division of Anatomic Pathology and Histology, Agostino Gemelli School of Medicine, Catholic University of the Sacred Heart, Largo Francesco Vito 1, Rome, 00168, Italy. FAU - Molinario, Clelia AU - Molinario C AD - Division of Anatomic Pathology and Histology, Agostino Gemelli School of Medicine, Catholic University of the Sacred Heart, Largo Francesco Vito 1, Rome, 00168, Italy. FAU - Benvenuto, Roberta AU - Benvenuto R AD - Division of Anatomic Pathology and Histology, Agostino Gemelli School of Medicine, Catholic University of the Sacred Heart, Largo Francesco Vito 1, Rome, 00168, Italy. FAU - Carlino, Angela AU - Carlino A AD - Unicamillus, International Medical University in Rome, Roma, Italy. FAU - Sacco, Emilio AU - Sacco E AD - Division of Urology, Agostino Gemelli School of Medicine, Catholic University of the Sacred Heart, Rome, Italy. FAU - Palermo, Giuseppe AU - Palermo G AD - Division of Urology, Agostino Gemelli School of Medicine, Catholic University of the Sacred Heart, Rome, Italy. FAU - Totaro, Angelo AU - Totaro A AD - Division of Urology, Agostino Gemelli School of Medicine, Catholic University of the Sacred Heart, Rome, Italy. FAU - Martini, Maurizio AU - Martini M AD - Department of Human Pathology in Adult and Developmental Age "Gaetano Barresi", Pathology Section, University of Messina, Messina, Italy. LA - eng PT - Journal Article DEP - 20261029 PL - Germany TA - Virchows Arch JT - Virchows Archiv : an international journal of pathology JID - 9423843 SB - IM OTO - NOTNLM OT - Bladder carcinoma OT - Methylation OT - Progression OT - Recurrence COIS- Declarations. Ethical approval: All clinicopathologic investigations were conducted according to the principles of the Declaration of Helsinki, and all information regarding the human material used in this study was managed using anonymous numerical codes. The study was approved by the local ethics committee of Messina (Prot. 24-25, 22/02/2024, AOU "G. Martino"). Informed consent: Informed consent was obtained from all subjects involved in the study. Conflict of interest: The authors declare no conflicts of interest that are directly or indirectly related to the work submitted for publication. Esther Diana Rossi, MD, PhD, is a member of the Editorial Board and discloses this relationship in accordance with journal policy. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:13 PHST- 2025/09/26 00:00 [received] PHST- 2026/06/18 00:00 [accepted] PHST- 2026/05/08 00:00 [revised] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:13 [entrez] AID - 10.1007/s00428-026-04626-8 [pii] AID - 10.1007/s00428-026-04626-8 [doi] PST - aheadofprint SO - Virchows Arch. 2026 Oct 29. doi: 10.1007/s00428-026-04626-8. PMID- 42955278 OWN - NLM STAT- Publisher LR - 20261029 IS - 1432-086X (Electronic) IS - 0174-1551 (Linking) DP - 2026 Oct 28 TI - Does Pain Phenotype Influence Clinical Outcomes After Genicular Artery Embolization? Movement-evoked Pain Versus Pain at Rest: A Retrospective Comparative Study. LID - 10.1007/s00270-026-04673-3 [doi] AB - PURPOSE: Pain in knee osteoarthritis is heterogeneous and may influence clinical response to genicular artery embolization (GAE). This study aimed to compare pain reduction after GAE between patients with substantial pain at rest (SPAR) and those with predominantly movement-evoked pain (PMEP). MATERIALS AND METHODS: This retrospective study included patients who underwent GAE for chronic knee pain and completed follow-up evaluations at 1 and 3 months. Patients were classified as SPAR when baseline VAS pain at rest was > 3 and as PMEP when VAS pain at rest was  40 years at disease onset, persistently high and treatment-refractory disease activity, dysphagia and cutaneous necrosis. Based on these factors, the individual cancer risk can be stratified and screening and follow-up examinations are recommended in accordance with the IMACS guidelines. DISCUSSION: The systematic early detection of malignant comorbidities in IIM is essential, as malignancies represent the leading cause of death in these patients. The recently published IMACS recommendations enable a standardized, risk-adapted approach to cancer screening and are intended to facilitate early diagnosis and treatment. CI - (c) 2026. The Author(s). FAU - Kastrati, Kastriot AU - Kastrati K AD - Klinische Abteilung fur Rheumatologie, Universitatsklinik fur Innere Medizin III, Wahringer Gurtel 18-20, 1090, Wien, Osterreich. AD - Klinische Abteilung fur Rheumatologie, Universitatsspital Oslo, Oslo, Norwegen. FAU - Gente, Karolina AU - Gente K AD - Innere Medizin V: Sektion Rheumatologie, Universitatsklinikum Heidelberg, Heidelberg, Deutschland. FAU - Hasseli-Frabel, Rebecca AU - Hasseli-Frabel R AD - Medizinische Klinik D, Sektion fur Rheumatologie und Klinische Immunologie, Universitatsklinikum Munster, Munster, Deutschland. AD - Institut fur Immunologie, Universitat Munster, Munster, Deutschland. FAU - Lechner-Radner, Helga AU - Lechner-Radner H AD - Klinische Abteilung fur Rheumatologie, Universitatsklinik fur Innere Medizin III, Wahringer Gurtel 18-20, 1090, Wien, Osterreich. helga.radner@meduniwien.ac.at. LA - ger PT - English Abstract PT - Journal Article PT - Review TT - Krebserkrankungen bei idiopathischen inflammatorischen Myopathien : Risikoabschatzung und Screeningstrategien. DEP - 20261028 PL - Germany TA - Z Rheumatol JT - Zeitschrift fur Rheumatologie JID - 0414162 SB - IM OTO - NOTNLM OT - Immuncheckpoint-Inhibitor OT - Malignancy OT - Myositis OT - Risk OT - Tumor screening COIS- Einhaltung ethischer Richtlinien. Interessenkonflikt: K. Kastrati: keine Interessenskonflikte bezogen auf diese Publikation. KK berichtet uber Honorare fur Vortrage und Prasentationen von Boehringer Ingelheim, Novartis, Eli Lilly und AbbVie sowie uber Unterstutzung fur die Teilnahme an Tagungen und/oder Reisekosten durch AbbVie, Johnson & Johnson und Eli Lilly. K. Gente: keine Interessenskonflikte bezogen auf diese Publikation. KG berichtet uber Honoraria fur Vortrage zu irAEs von AbbVie, BMS, Galapagos, Janssen, Lilly, MSD, Novartis, Sandoz, UCB und Viatris. R. Hasseli-Frabel: keine Interessenskonflikte bezogen auf diese Publikation. H. Lechner-Radner: keine Interessenskonflikte bezogen auf diese Publikation. Fur diesen Beitrag wurden von den Autor/-innen keine Studien an Menschen oder Tieren durchgefuhrt. Fur die aufgefuhrten Studien gelten die jeweils dort angegebenen ethischen Richtlinien. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:13 PHST- 2026/03/05 00:00 [received] PHST- 2026/03/27 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:13 [entrez] AID - 10.1007/s00393-026-01877-3 [pii] AID - 10.1007/s00393-026-01877-3 [doi] PST - aheadofprint SO - Z Rheumatol. 2026 Oct 28. doi: 10.1007/s00393-026-01877-3. PMID- 42955256 OWN - NLM STAT- Publisher LR - 20261029 IS - 1874-9356 (Electronic) IS - 0015-5632 (Linking) DP - 2026 Oct 29 TI - Gut microbiota as a key player in diabetes mellitus: mechanisms, dysbiosis, and emerging interventions. LID - 10.1007/s12223-026-01618-8 [doi] AB - The gut microbiome has emerged as a critical regulator of metabolic and immune homeostasis, with significant implications for diabetes mellitus. This narrative review synthesizes findings from selected landmark original investigations published from 2000 to 2026, examining the role of the gut microbiome in both type 1 diabetes (T1D) and type 2 diabetes (T2D). As a narrative rather than a systematic review, this synthesis does not follow a registered protocol or an exhaustive, reproducible search strategy. Observational studies frequently report dysbiosis, characterized by reduced microbial diversity and altered taxa, as a feature of both prediabetes and diabetes, correlating with poor glycemic control and complications, although the specific taxa and dysbiotic signatures implicated vary across geographically and ethnically distinct populations, indicating that dysbiosis is not a uniform, population-invariant hallmark of disease. Mechanistic studies highlight pathways such as short-chain fatty acid production, immune modulation, and gut barrier integrity, which mediate microbial effects on insulin sensitivity and glucose metabolism, with short-chain fatty acid (SCFA) signaling and gut barrier integrity identified as the most significant pathways driving these effects. Interventional approaches, including probiotics, prebiotics, dietary modifications, fasting, exercise, and bariatric surgery, show preliminary, proof-of-concept potential to reshape the microbiome and improve diabetic outcomes, although none of these interventions has yet been validated in large-scale, adequately powered, multi-center randomized controlled trials with hard clinical endpoints, and none should be regarded as an established treatment. Notably, T1D research emphasizes autoimmune interactions, while T2D focuses on metabolic dysfunction. Despite advances, challenges like establishing causality and addressing inter-individual variability persist. This review underscores the microbiome's therapeutic promise pending confirmation in the larger trials outlined above, and advocates for personalized, multi-omics-driven strategies to enhance diabetes management. CI - (c) 2026. Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. FAU - Bastaminejad, Saiyad AU - Bastaminejad S AUID- ORCID: 0000-0001-6722-6432 AD - Department of Genetics and Molecular Medicine, School of ParaMedicine, Ilam University of Medical Sciences, Ilam, Iran. FAU - Bakhtiyari, Salar AU - Bakhtiyari S AUID- ORCID: 0000-0002-2500-269X AD - Department of Molecular Pharmacology and Physiology, Morsani College of Medicine, University of South Florida, Tampa, FL, USA. AD - Department of Clinical Biochemistry, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran. FAU - Heidari, Amin AU - Heidari A AD - Department of Pharmacology, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran. FAU - Ebrahimi, Esmaeil AU - Ebrahimi E AUID- ORCID: 0000-0001-5103-5789 AD - Department of Biology, Faculty of Basic Sciences, Payame Noor University, Ilam, Iran. e.ebrahimi.biology@gmail.com. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - United States TA - Folia Microbiol (Praha) JT - Folia microbiologica JID - 0376757 SB - IM OTO - NOTNLM OT - Diabetes mellitus OT - Dysbiosis OT - Glucose metabolism OT - Gut microbiome COIS- Declarations. Ethics approval: Not applicable. Consent for publication: Not applicable. Consent to participate: Not applicable. Competing interests: The authors declare no competing interests. Clinical trial number: Not applicable. Use of AI and AI-assisted technologies: During the preparation of this manuscript, the authors used Claude (Sonnet 5, Anthropic) for language editing and stylistic refinement. All AI-assisted text was critically reviewed, edited, and verified by the authors, who take full responsibility for the content and scientific accuracy of the final manuscript. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:13 PHST- 2026/07/19 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:13 [entrez] AID - 10.1007/s12223-026-01618-8 [pii] AID - 10.1007/s12223-026-01618-8 [doi] PST - aheadofprint SO - Folia Microbiol (Praha). 2026 Oct 29. doi: 10.1007/s12223-026-01618-8. PMID- 42955254 OWN - NLM STAT- Publisher LR - 20261029 IS - 1179-1888 (Electronic) IS - 1175-0561 (Linking) DP - 2026 Oct 28 TI - Evidence-Based Alternative Strategies to Improve and Restore Skin Barrier Function: A Narrative Review. LID - 10.1007/s40257-026-01071-w [doi] AB - The skin barrier is a complex system composed of several functional layers (physical, chemical, microbiological, and immunological) that interact to protect the body from the environment while maintaining overall homeostasis. Proper functioning of the skin barrier can be disturbed by intrinsic as well as extrinsic factors, leading to the development of cutaneous disorders such as skin sensitivity, and inflammatory diseases such as atopic dermatitis. Skin moisturization is a key first-line strategy to maintain skin health and treat dermatological conditions associated with impaired skin barrier function. Beyond conventional topical therapies, alternative or complementary approaches have demonstrated potential in improving and restoring the function of the skin barrier. This narrative review summarizes key insights from proceedings of an international meeting on skin barrier function, during which various strategies for improving or restoring skin barrier integrity and relieving the potential consequences of skin barrier damage (e.g., dryness, irritation, and itching) were discussed. In particular, evidence presented supporting the effectiveness of nutraceuticals is described. This review also examines how using lasers to deliberately disrupt the skin barrier in a controlled manner can promote restoration via physiological skin repair mechanisms, as well as facilitate topical drug delivery. These findings underscore the need for continued research to allow for a more patient-centered approach to managing barrier dysfunction and optimizing skin care regimens. CI - (c) 2026. The Author(s). FAU - Farris, Patricia K AU - Farris PK AUID- ORCID: 0000-0003-4349-0129 AD - Department of Dermatology, Tulane University School of Medicine, 1430 Tulane Ave, New Orleans, LA, 70112, USA. pkfarmd@gmail.com. FAU - Campos, Valeria AU - Campos V AUID- ORCID: 0000-0002-3350-8586 AD - Department of Dermatology, Jundiai Medicine College, Sao Paulo, Brazil. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - New Zealand TA - Am J Clin Dermatol JT - American journal of clinical dermatology JID - 100895290 SB - IM COIS- Declarations. Conflicts of interest/competing interests: Patricia K. Farris has served as a medical advisory board member and/or consultant for AlumierMD, Beiersdorf, Bubble Beauty, Cerave, Galderma, Estee Lauder, Fig-1 Skincare, Galderma, Kenvue, La Roche Posay, Nutraceutical Wellness, One Skin, Pierre Fabre, Rationale, Sente, Skinbetter Science, Skinmedica, and Vichy, but has no financial interests in any of the stated companies, no personal relationships with any of the stated companies, and no conflict of interest from these interactions with the pharma industry. She is a co-founder and stockholder of RegimenMD. Valeria Campos has been a scientific advisor for Bioderma, Galderma, LMG, L'Oreal, Naos, and Vydence, but has no financial interests in any of the stated companies, no personal relationships with any of the stated companies, and no conflict of interest from these interactions with the pharma industry. The 'Entretiens d'Avene' conference is held annually for scientific and educational purposes, with no promotion of products and services. Moreover, the sponsor had no role in the literature search or study selection, or in the analysis and interpretation of data. Ethics approval: Not applicable. Consent to participate: Not applicable. Consent for publication: The patient provided informed consent for publication of the images in Fig. 2. Availability of data and material: Not applicable. Code availability: Not applicable. Authors' contributions: PKF and VC contributed to the conception of the review, performed the literature search, analyzed the data, critically revised the manuscript, and approved its final version. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:13 PHST- 2026/03/26 00:00 [received] PHST- 2026/09/07 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:13 [entrez] AID - 10.1007/s40257-026-01071-w [pii] AID - 10.1007/s40257-026-01071-w [doi] PST - aheadofprint SO - Am J Clin Dermatol. 2026 Oct 28. doi: 10.1007/s40257-026-01071-w. PMID- 42955234 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1708-3923 (Electronic) IS - 0383-6320 (Linking) VI - 51 IP - 3 DP - 2026 Fall TI - [Integrating jungian concepts into ACT: Toward a deepening of experiential work]. PG - 71-82 AB - This article proposes a clinical intervention integrating selected concepts from Jungian analytical psychology into Acceptance and Commitment Therapy (ACT; Hayes et al., 2012). ACT aims to enhance psychological flexibility through the acceptance of internal experiences, cognitive defusion, mindful awareness, and committed action guided by personal values. Although the effectiveness of ACT has been well established across a wide range of clinical contexts, the approach may benefit from the inclusion of symbolic and relational dimensions capable of deepening experiential work. Jungian analytical psychology, particularly through the concepts of archetypes, the shadow, the persona, and individuation, provides a complementary framework for understanding certain symbolic and identity-related dynamics involved in psychological change. The proposed integration, conceptualized as an assimilative integration within an ACT framework, is illustrated through clinical vignettes showing how Jungian concepts can support experiential work. Jungian concepts are primarily mobilized here as clinical and metaphorical reference points intended to enrich experiential exploration and identity-related reflection within an ACT framework. This dialogue between ACT and Jungian psychology opens new possibilities for a clinical practice that combines symbolic depth with process-based intervention. The article concludes with a discussion of the clinical implications and research perspectives associated with this integrative approach. FAU - Dionne, Frederick AU - Dionne F AD - Universite du Quebec a Trois-Rivieres, Quebec, Canada. LA - fre PT - English Abstract PT - Journal Article TT - Integrer certains concepts jungiens a l'ACT : vers un approfondissement du travail experientiel. PL - Canada TA - Sante Ment Que JT - Sante mentale au Quebec JID - 9424773 SB - IM MH - *Jungian Theory MH - Humans EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:12 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:12 [entrez] AID - 1129187ar [pii] PST - ppublish SO - Sante Ment Que. 2026 Fall;51(3):71-82. PMID- 42955224 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2191-1363 (Electronic) IS - 2191-1363 (Linking) VI - 15 IP - 11 DP - 2026 Nov TI - Machine Learning and Structural Analysis for Generation-Wise Selection of Perylene Diimide Dendrimers Based on Topological Compatibility With Drug Molecules. LID - e70324 [pii] LID - 10.1002/open.70324 [doi] AB - Machine learning is increasingly used to explore structure-property relationships in dendrimer-drug systems; however, its integration with topology-based structural compatibility analysis remains limited. This study presents a learning-driven framework to evaluate the topology-based structural compatibility of perylene diimide core dendrimers with antiviral, antioxidant, and imaging agents. Within a quantitative structure-property relationship framework, linear regression, Gaussian process regression (GPR), and artificial neural networks are employed to identify dominant graph-theoretic topological indices and to capture linear and nonlinear structure-property relationships. These insights are combined with a structure-based compatibility analysis that ranks dendrimer generations without altering drug identity or ordering. Generation-wise compatibility is computed using standardized Euclidean distance after global normalization, and ranking stability is assessed through monotonic and magnitude analysis. Results show class-dependent descriptor relevance, with GPR achieving test-set R(2) values above 0.94 for molecular weight, exact mass, monoisotopic mass, and heavy atom count. The generation-wise compatibility analysis further reveals distinct topology-based structural compatibility patterns across the investigated drug classes, highlighting the value of integrating machine learning with graph-theoretic analysis for rational nanocarrier design and the computational assessment of potential encapsulation suitability. CI - (c) 2026 The Authors. ChemistryOpen published by Wiley-VCH GmbH. FAU - Bibi, Zainab AU - Bibi Z AD - Department of Basic Sciences & Humanities, College of Electrical & Mechanical Engineering, National University of Sciences & Technology, Islamabad, Pakistan. AD - Department of Mathematics, Department of Computer Science, Department of Software Engineering, National University of Modern Languages, Rawalpindi, Islamabad, Pakistan. FAU - Ali, Jabbar AU - Ali J AUID- ORCID: 0009-0000-9310-3276 AD - Department of Basic Sciences & Humanities, College of Electrical & Mechanical Engineering, National University of Sciences & Technology, Islamabad, Pakistan. AD - Department of Mathematics, Department of Computer Science, Department of Software Engineering, National University of Modern Languages, Rawalpindi, Islamabad, Pakistan. FAU - Irshad, Sana AU - Irshad S AD - Department of Basic Sciences & Humanities, College of Electrical & Mechanical Engineering, National University of Sciences & Technology, Islamabad, Pakistan. AD - Department of Mathematics, Department of Computer Science, Department of Software Engineering, National University of Modern Languages, Rawalpindi, Islamabad, Pakistan. FAU - Ghafar, Muhammad Saad AU - Ghafar MS AUID- ORCID: 0000-0001-7588-4902 AD - Department of Mathematics, Department of Computer Science, Department of Software Engineering, National University of Modern Languages, Rawalpindi, Islamabad, Pakistan. FAU - Ali, Yasir AU - Ali Y AUID- ORCID: 0000-0003-0234-6817 AD - Department of Basic Sciences & Humanities, College of Electrical & Mechanical Engineering, National University of Sciences & Technology, Islamabad, Pakistan. FAU - Shang, Yilun AU - Shang Y AUID- ORCID: 0000-0002-2817-3400 AD - School of Computer Science, Northumbria University, Newcastle upon Tyne, UK. LA - eng PT - Journal Article PL - Germany TA - ChemistryOpen JT - ChemistryOpen JID - 101594811 RN - 0 (Dendrimers) RN - 0 (Imides) RN - 5QD5427UN7 (Perylene) RN - 0 (perylenediimide) RN - 0 (Antioxidants) RN - 0 (Antiviral Agents) SB - IM MH - *Dendrimers/chemistry MH - *Imides/chemistry MH - *Perylene/analogs & derivatives/chemistry MH - *Machine Learning MH - Quantitative Structure-Activity Relationship MH - Neural Networks, Computer MH - Antioxidants/chemistry MH - Antiviral Agents/chemistry OTO - NOTNLM OT - PDI core dendrimers OT - QSPR modeling Gaussian process regression OT - drug encapsulation compatibility OT - graph-theoretic topological indices EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:12 PHST- 2026/09/21 00:00 [revised] PHST- 2026/04/22 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:12 [entrez] AID - e70324 [pii] AID - 10.1002/open.70324 [doi] PST - ppublish SO - ChemistryOpen. 2026 Nov;15(11):e70324. doi: 10.1002/open.70324. PMID- 42955223 OWN - NLM STAT- Publisher LR - 20261029 IS - 1573-7330 (Electronic) IS - 1058-0468 (Linking) DP - 2026 Oct 28 TI - Artificial oocyte activation in patients with fertilization failure using calcium ionophores: a meta-analysis on reproductive outcomes. LID - 10.1007/s10815-026-04064-0 [doi] AB - PURPOSE: To evaluate the clinical efficacy and safety of artificial oocyte activation using calcium ionophores on reproductive and perinatal outcomes in couples with a history of fertilization failure following ICSI. METHODS: A systematic search was conducted for English-language studies published between 2003 and 2025. Studies comparing AOA-treated groups using calcium ionophores versus non-treated ICSI controls were included. Subgroup analyses were performed based on study design, type of ionophore (ionomycin vs. calcimycin), and fertilization failure subtype (total vs. low fertilization). Risk of bias was assessed using Cochrane RoB 2 and ROBINS-I tools. RESULTS: Twenty-one studies met inclusion criteria. ICSI-AOA using calcium ionophores was associated with an increased fertilization rate, clinical pregnancy rate, and live birth rate. Miscarriage rate was reduced. Subgroup analyses indicated that ionomycin was more effective than calcimycin in improving fertilization, and that benefits were also observed for total and low fertilization failure. No differences were found for perinatal characteristics or malformation rates. Risk of bias was moderate to high, and no evidence of publication bias was detected. CONCLUSION: Calcium ionophore-mediated activation may enhance reproductive outcomes in ICSI cycles in patients with fertilization failure, without increasing miscarriage or adverse neonatal outcomes. Their selective use could prevent cycle cancellation and reduce the emotional and financial burden associated with repeated ICSI failures. However, given the variability in ionophore protocols and experimental design, further randomized studies are warranted to standardize dosing, timing, and indications before universal clinical adoption. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - de Celis, Carlos AU - de Celis C AD - IVIRMA Global Research Alliance, IVI Foundation, Valencia, Spain. carlos.decelis@ivirma.com. FAU - Gil, Julia AU - Gil J AD - IVIRMA Global Research Alliance, IVI Foundation, Valencia, Spain. FAU - Madero, Maria AU - Madero M AD - IVIRMA Global Research Alliance, IVI Foundation, Instituto de Investigacion Sanitaria La Fe (IIS La Fe), Valencia, Spain. FAU - Ortega-Jaen, David AU - Ortega-Jaen D AD - IVIRMA Global Research Alliance, IVI Foundation, Valencia, Spain. FAU - Pardinas, Maria Luisa AU - Pardinas ML AD - IVIRMA Global Research Alliance, IVI Foundation, Valencia, Spain. FAU - Martin, Angel AU - Martin A AD - IVIRMA Global Research Alliance, IVI Foundation, Valencia, Spain. FAU - Garrido, Nicolas AU - Garrido N AD - IVIRMA Global Research Alliance, IVI Foundation, Instituto de Investigacion Sanitaria La Fe (IIS La Fe), Valencia, Spain. AD - Faculty of Health Sciences, Department of Biosciences, Universidad Europea de Valencia, Valencia, Spain. FAU - de Los Santos, Maria Jose AU - de Los Santos MJ AD - IVIRMA Global IVF Laboratory, Valencia, Spain. mariajose.delossantos@ivirma.com. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - J Assist Reprod Genet JT - Journal of assisted reproduction and genetics JID - 9206495 SB - IM OTO - NOTNLM OT - Artificial oocyte activation OT - Calcium ionophore OT - Fertilization failure OT - Intracytoplasmic sperm injection COIS- Declarations. Ethics approval and consent to participate: This study was conducted in full accordance with the ethical principles for medical research involving human subjects outlined in the Declaration of Helsinki, as well as applicable national regulations and institutional guidelines for retrospective observational studies. Ethical approval and waiver of formal informed consent (due to the retrospective, anonymized nature of the meta-analyzed data) were granted by the Research Ethics Committee of Instituto Valenciano de Infertilidad (CEIm IVI Valencia, Spain) under the approved protocol reference number 2503-VLC-035-MD. Consent to participate: Formal informed consent was waived by the ethics committee due to the retrospective nature of the study. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. Attestation statement: Data regarding any of the subjects in the study has not been previously published. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:12 PHST- 2026/04/02 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:12 [entrez] AID - 10.1007/s10815-026-04064-0 [pii] AID - 10.1007/s10815-026-04064-0 [doi] PST - aheadofprint SO - J Assist Reprod Genet. 2026 Oct 28. doi: 10.1007/s10815-026-04064-0. PMID- 42955218 OWN - NLM STAT- Publisher LR - 20261029 IS - 1741-0444 (Electronic) IS - 0140-0118 (Linking) DP - 2026 Oct 28 TI - Predicting in-silico ventricular tachycardia induction on fuzzy cardiac digital twins. LID - 10.1007/s11517-026-03699-6 [doi] AB - Ventricular tachycardia (VT) is a leading cause of sudden cardiac death (SCD). To date, clinical VT risk assessment is either inaccurate, invasive or expensive. Patient-specific computer modeling of heart electrophysiology (EP) is a promising non-invasive risk assessment approach. It has three major limitations: computational cost, inaccurate arrhythmogenic substrate geometry and unknown patient-specific tissue EP properties. We present a pacing outcome predictor (POP) that quickly identifies the conditions needed to induce VT in three-dimensional personalized cardiac computer models. The POP is used to test VT induction across multiple model versions of a patient, covering scar geometry and EP property variations, with an added layer of model reduction to further accelerate calculations. The results are combined into a personalized VT risk metric based on in-silico VT inducibility across the patient's models. In a retrospective 10-patient repaired Tetralogy of Fallot cohort, the metric showed complete separation (10/10) between patients with and without clinically inducible VT, suggesting potential for non-invasive VT risk assessment in this population. CI - (c) 2026. International Federation for Medical and Biological Engineering. FAU - Crabbe, Olivier AU - Crabbe O AUID- ORCID: 0009-0009-6046-5858 AD - Universite de Rennes, CHU Rennes, Inserm, LTSI - UMR 1099, Rennes, France. olivier.crabbe@etudiant.univ-rennes.fr. AD - Synopsys, Inc., Sunnyvale, CA, 94085, USA. olivier.crabbe@etudiant.univ-rennes.fr. FAU - Bessiere, Francis AU - Bessiere F AD - Service de Rythmologie, Institut de Cardiologie, Hopital Louis Pradel, Hospices Civils de Lyon, Bron, France. AD - Departement Medico-Chirurgical de Cardiologie Congenitale du Foetus, de l'Enfant a l'Adulte, Hopital Louis Pradel, Hospices Civils de Lyon, Bron, France. AD - LabTAU, INSERM U1032, Universite Claude Bernard Lyon 1, Lyon, France. FAU - L'Eplattenier, Pierre AU - L'Eplattenier P AD - Synopsys, Inc., Sunnyvale, CA, 94085, USA. FAU - Grivot, Christelle AU - Grivot C AD - Synopsys, Inc., Sunnyvale, CA, 94085, USA. FAU - Ditac, Geoffroy AU - Ditac G AD - Service de Rythmologie, Institut de Cardiologie, Hopital Louis Pradel, Hospices Civils de Lyon, Bron, France. AD - LabTAU, INSERM U1032, Universite Claude Bernard Lyon 1, Lyon, France. FAU - Rochette, Michel AU - Rochette M AD - Synopsys, Inc., Sunnyvale, CA, 94085, USA. FAU - Martins, Raphael AU - Martins R AD - Universite de Rennes, CHU Rennes, Inserm, LTSI - UMR 1099, Rennes, France. FAU - Haigron, Pascal AU - Haigron P AD - Universite de Rennes, CHU Rennes, Inserm, LTSI - UMR 1099, Rennes, France. FAU - El Houari, Karim AU - El Houari K AD - Synopsys, Inc., Sunnyvale, CA, 94085, USA. LA - eng GR - 2023/0299/Association Nationale de la Recherche et de la Technologie/ PT - Journal Article DEP - 20261028 PL - United States TA - Med Biol Eng Comput JT - Medical & biological engineering & computing JID - 7704869 SB - IM OTO - NOTNLM OT - Computational modeling OT - Risk stratification OT - Tetralogy of Fallot OT - Ventricular tachycardia COIS- Declarations. Conflicts of interest: OC, PLE, CG, MR and KEH are employed by Synopsys Inc. (legacy Ansys Inc.). Ethical approval: This retrospective study involving human participants was in accordance with the ethical standards of the institutional and national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. The Scientific and Ethical Committee of Hospices Civils de Lyon approved this study. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:12 PHST- 2026/04/13 00:00 [received] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:12 [entrez] AID - 10.1007/s11517-026-03699-6 [pii] AID - 10.1007/s11517-026-03699-6 [doi] PST - aheadofprint SO - Med Biol Eng Comput. 2026 Oct 28. doi: 10.1007/s11517-026-03699-6. PMID- 42955217 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1534-6242 (Electronic) IS - 1523-3804 (Linking) VI - 28 IP - 1 DP - 2026 Oct 29 TI - Highlights of Cardiovascular Disease Prevention Studies Presented at the 2026 European Society of Cardiology Conference. LID - 105 [pii] LID - 10.1007/s11883-026-01481-5 [doi] AB - PURPOSE OF REVIEW: This review summarizes key cardiovascular disease prevention studies presented at the 2026 European Society of Cardiology (ESC) Congress, highlighting new evidence in lipid management, cardiovascular risk assessment, blood pressure control, and vaccination. RECENT FINDINGS: Several studies addressed persistent gaps in lipid management and atherosclerotic cardiovascular disease prevention. STAREE showed that atorvastatin reduced major cardiovascular events in adults aged 70 years and older without established cardiovascular disease, although it did not significantly improve disability-free survival. The SHASTA-3 and SHASTA-4 trials showed that plozasiran produced substantial and sustained triglyceride reductions and reduced acute pancreatitis events in patients with severe hypertriglyceridemia. VICTORION-Challenge demonstrated greater LDL-C reduction and target attainment with inclisiran compared with bempedoic acid in high- and very-high-risk patients receiving background statin therapy. In patients with homozygous familial hypercholesterolemia, VSA003 substantially lowered LDL-C and several other atherogenic lipoproteins by inhibiting ANGPTL3. Two studies examined imaging-based approaches to preventive therapy: RESPECT2 showed that coronary computed tomography angiography-guided lipid management improved adherence compared with conventional risk-score-based treatment, while CorCal found that coronary artery calcium-guided statin recommendations reduced the number of patients selected for treatment and were associated with greater LDL-C lowering and higher reported adherence, although noninferiority for cardiovascular events was not established. Beyond lipid management, long-term follow-up of the China Rural Hypertension Control Project showed that intensive blood pressure management led by non-physician healthcare providers reduced dementia and cognitive impairment. TIMER demonstrated sustained LDL-C lowering with once-monthly ticalicibart in patients with nonfamilial hypercholesterolemia receiving background statin therapy, while TRANQUILITY demonstrated sustained reductions in high-sensitivity C-reactive protein with the interleukin-6 (IL-6) inhibitor pacibekitug in patients with chronic kidney disease. DAN-RSV demonstrated that RSVpreF vaccination reduced RSV-related respiratory and cardiorespiratory hospitalizations across two seasons. Studies presented at ESC 2026 broadened the evidence base for cardiovascular prevention across pharmacologic therapy, imaging-guided risk assessment, blood pressure management, and vaccination. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Abdul Jabbar, Ali Bin AU - Abdul Jabbar AB AUID- ORCID: 0000-0001-5145-8626 AD - Department of Medicine, Division of Cardiovascular Disease, Creighton University School of Medicine, Omaha, NE, USA. FAU - Zulfiqar, Kalsoom AU - Zulfiqar K AD - Department of Pathology, Aga Khan University Medical College, Karachi, Pakistan. FAU - Malik, Shahzil Abdur Rehman AU - Malik SAR AD - Department of Medicine, Internal Medicine Division, Creighton University School of Medicine, Omaha, NE, USA. FAU - Inam, Maha AU - Inam M AD - Department of Internal Medicine, Yale School of Medicine, New Haven, CT, USA. FAU - Fazal, Wahab AU - Fazal W AD - Department of Medicine, Internal Medicine Division, Creighton University School of Medicine, Omaha, NE, USA. FAU - Hyder, Ali AU - Hyder A AD - Department of Medicine, Internal Medicine Division, Creighton University School of Medicine, Omaha, NE, USA. FAU - Minhas, Abdul Mannan Khan AU - Minhas AMK AD - Section of Cardiology, Department of Medicine, Baylor College of Medicine, Houston, TX, USA. FAU - Slipczuk, Leandro AU - Slipczuk L AD - Division of Cardiology, Montefiore Health System/Albert Einstein College of Medicine, Bronx, NY, USA. FAU - Krittanawong, Chayakrit AU - Krittanawong C AD - HumanX, Delaware, DE, USA. FAU - Kalra, Dinesh K AU - Kalra DK AD - Rudd Heart & Lung Center, University of Louisville School of Medicine, Louisville, KY, USA. FAU - Virani, Salim S AU - Virani SS AD - Department of Medicine, Aga Khan University Medical College, Karachi, Pakistan. salim.virani@aku.edu. AD - Department of Population Health, Aga Khan University Medical College, Stadium Road, PO Box 3500, Nairobi, Kenya. salim.virani@aku.edu. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - United States TA - Curr Atheroscler Rep JT - Current atherosclerosis reports JID - 100897685 RN - 0 (Hydroxymethylglutaryl-CoA Reductase Inhibitors) SB - IM MH - Humans MH - *Cardiovascular Diseases/prevention & control MH - *Cardiology MH - Europe MH - Hydroxymethylglutaryl-CoA Reductase Inhibitors/therapeutic use MH - Societies, Medical OTO - NOTNLM OT - Cardiovascular disease OT - Hypertension OT - Inflammation OT - PCSK9 OT - Prevention OT - Vaccination COIS- Declarations. Competing interests: SSV has received research funding from the National Institutes of Health, US Department of Veterans Affairs, NIHR UK, Tahir and Jooma Family, and the Asharia Family. Other authors declare no competing interests.LZ has received honoraria from Elsevier as Editor-in-Chief of Progress in Cardiovascular Diseases. Institutional grants from Amgen and PhilipsDr. Krittanawong is the founder of HumanX, a company that focuses on artificial intelligence in precision medicine and innovation in healthcare; he declares that his affiliation with HumanX is not a competing interest. Human and Animal Rights and Informed Consent: The authors did not use any animal or human subjects in this study. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:12 PHST- 2026/09/26 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:12 [entrez] AID - 10.1007/s11883-026-01481-5 [pii] AID - 10.1007/s11883-026-01481-5 [doi] PST - epublish SO - Curr Atheroscler Rep. 2026 Oct 29;28(1):105. doi: 10.1007/s11883-026-01481-5. PMID- 42955209 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1941-6636 (Electronic) VI - 57 IP - 1 DP - 2026 Oct 28 TI - Efficacy and Safety of Short-Course Radiotherapy Alone or Combined With Consolidation Chemotherapy Versus Conventional Long-Course Chemoradiotherapy for Locally Advanced Rectal Cancer: A Meta-Analysis of Randomized Controlled Trials. LID - 248 [pii] LID - 10.1007/s12029-026-01606-8 [doi] AB - OBJECTIVE: Short-course radiotherapy (SCRT)-based regimens, including SCRT-alone and SCRT-based total neoadjuvant therapy (SCRT-based TNT, defined as SCRT followed by consolidation chemotherapy), have emerged as alternative neoadjuvant options to long-course chemoradiotherapy (LCCRT) for locally advanced rectal cancer (LARC). This meta-analysis stratified analyses by the type of SCRT regimen and whether LCCRT arms received preoperative consolidation chemotherapy, to systematically compare their efficacy and safety and inform personalized treatment selection. METHODS: A systematic search of PubMed, Embase, Cochrane Library, and Web of Science identified randomized controlled trials (RCTs) comparing SCRT-based regimens and LCCRT up to November 2025. Two reviewers independently performed study selection, data extraction, and risk-of-bias assessment. Pooled analyses were conducted using Review Manager 5.3, with relative risk (RR) or hazard ratio (HR) and 95% confidence intervals (CIs). RESULTS: Nine randomized trials (reported in 16 publications), involving 3,061 patients, were included. In the primary comparison of SCRT-based TNT versus standard LCCRT, the SCRT-based TNT group achieved a significantly higher pathological complete response rate (RR = 1.56, 95% CI: 1.24-1.97, P = 0.0002), whereas tumor downstaging and locoregional failure showed no significant differences from the LCCRT group. The SCRT-based TNT regimen was associated with a significantly higher incidence of grade >/= 3 acute toxicity (RR = 1.63, 95% CI: 1.14-2.34, P = 0.008), and showed numerical trends toward improved overall survival (HR = 0.99, 95% CI: 0.76-1.28, P = 0.91) and disease-free survival (HR = 1.05, 95% CI: 0.77-1.43, P = 0.77) that did not reach statistical significance. SCRT alone was associated with lower pCR (RR = 0.13, 95% CI: 0.03-0.51, P = 0.003) and downstaging rates (RR = 0.66, 95% CI: 0.56-0.79, P < 0.00001) but a markedly lower incidence of acute toxicity (RR = 0.12, 95% CI: 0.05-0.29, P < 0.00001). An exploratory analysis based on a single small trial found no significant differences between SCRT-based TNT and LCCRT-based TNT across all major endpoints. Pooling all SCRT-based strategies obscured clinically meaningful heterogeneity and yielded no significant differences for any primary endpoint. CONCLUSIONS: SCRT-based TNT significantly improves pCR compared with standard LCCRT but does not translate into survival benefit, with a numerical increase in locoregional failure. SCRT-alone offers lower acute toxicity but substantially poorer tumor response, warranting use only in selected patients. The comparison between SCRT-based TNT and LCCRT-based TNT remains inconclusive. SCRT-based regimens represent heterogeneous treatment pathways rather than a uniform substitute for LCCRT, requiring individualized clinical decision-making. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Wang, Chaoyang AU - Wang C AD - Department of Colorectal and Anal Surgery, Huaihe Hospital of Henan University, Kaifeng, 475004, China. wangchaoyang10475@163.com. FAU - Han, Yaguang AU - Han Y AD - Department of Colorectal and Anal Surgery, Huaihe Hospital of Henan University, Kaifeng, 475004, China. FAU - Xue, Shuxin AU - Xue S AD - Department of Colorectal and Anal Surgery, Huaihe Hospital of Henan University, Kaifeng, 475004, China. FAU - Liu, Kejia AU - Liu K AD - Department of Colorectal and Anal Surgery, Huaihe Hospital of Henan University, Kaifeng, 475004, China. FAU - Zhang, Zhenlin AU - Zhang Z AD - Department of Colorectal and Anal Surgery, Huaihe Hospital of Henan University, Kaifeng, 475004, China. FAU - Li, Yong AU - Li Y AD - Department of Thoracic Surgery, Affiliated Hospital Group of Guangdong Medical University Shenzhen Baoan Central Hospital (Baoan Central Hospital of Shenzhen), Shenzhen, Guangdong, 516003, China. langebai@163.com. LA - eng PT - Comparative Study PT - Journal Article PT - Meta-Analysis PT - Review DEP - 20261028 PL - United States TA - J Gastrointest Cancer JT - Journal of gastrointestinal cancer JID - 101479627 SB - IM MH - Humans MH - *Rectal Neoplasms/therapy/pathology/mortality MH - Randomized Controlled Trials as Topic MH - *Chemoradiotherapy/methods/adverse effects MH - *Neoadjuvant Therapy/methods MH - *Consolidation Chemotherapy/methods MH - Pathologic Complete Response MH - Treatment Outcome OTO - NOTNLM OT - Locally advanced rectal cancer OT - Long-course chemoradiotherapy OT - Meta-analysis OT - Randomized controlled trial OT - Short-course radiotherapy OT - Total neoadjuvant therapy COIS- Declarations. Ethics approval and consent to participate: This meta-analysis synthesizes published aggregate RCT data and does not require new ethical approval; all original included trials obtained institutional review board approval and written patient informed consent as documented in their original publications. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:12 PHST- 2026/06/15 00:00 [received] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:12 [entrez] AID - 10.1007/s12029-026-01606-8 [pii] AID - 10.1007/s12029-026-01606-8 [doi] PST - epublish SO - J Gastrointest Cancer. 2026 Oct 28;57(1):248. doi: 10.1007/s12029-026-01606-8. PMID- 42955203 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1534-6269 (Electronic) IS - 1523-3790 (Linking) VI - 28 IP - 1 DP - 2026 Oct 28 TI - Gut Microbiome Signatures and Immune Checkpoint Inhibitor Response in Gastrointestinal Cancers: Reproducibility and Translational Readiness. LID - 114 [pii] LID - 10.1007/s11912-026-01853-9 [doi] AB - PURPOSE OF REVIEW: Gut microbial features have been associated with immune checkpoint inhibitor (ICI) outcomes, but whether these signatures are robust across gastrointestinal (GI) tumor types, treatment regimens, and populations remains uncertain. This critical narrative review assesses human evidence, experimental mechanisms, longitudinal sampling, and the criteria needed for clinical implementation. RECENT FINDINGS: A gastric cancer cohort showed different response-associated microbial features for immunotherapy alone, chemotherapy alone, and combined treatment. Cross-cancer strain-resolved analyses found that signature transportability was strongly conditional on matching ICI regimens. A large primary liver cancer cohort analyzed 315 patients and 777 serial samples and explored a baseline score derived from on-treatment species selection; later samples were available only in a selected subgroup, complicating interpretation. Experimental studies link selected microbes and metabolites with antitumor immunity, but these mechanistic findings do not establish therapeutic efficacy in humans. Fecal microbiota transplantation (FMT) studies in melanoma demonstrate feasibility yet do not establish benefit in GI malignancies. Stool profiles may not represent mucosal or tumor-adjacent communities in upper GI cancer. No stool microbiome signature is currently validated to select ICI therapy in GI oncology. Meaningful translation requires standardized assays, non-ICI comparators to establish treatment-effect prediction, strict separation of discovery and external validation, prospectively handled missingness, and randomized clinical-utility studies. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Sagawa, Tamotsu AU - Sagawa T AUID- ORCID: 0000-0002-1994-4795 AD - Department of Gastroenterology, National Hospital Organization Hokkaido Cancer Center, i inverted question mark inverted question mark003-0804 2-3-54 Kikusui 4-jo, Shiroishi-ku, Sapporo, Hokkaido, Japan. stamotsu@jk9.so-net.ne.jp. FAU - Hirakawa, Masahiro AU - Hirakawa M AD - Division of Medical Oncology, Department of Internal Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan. FAU - Nagashima, Hiroyuki AU - Nagashima H AD - Department of Gastroenterology, National Hospital Organization Hokkaido Cancer Center, i inverted question mark inverted question mark003-0804 2-3-54 Kikusui 4-jo, Shiroishi-ku, Sapporo, Hokkaido, Japan. FAU - Fujikawa, Koshi AU - Fujikawa K AD - Department of Gastroenterology, National Hospital Organization Hokkaido Cancer Center, i inverted question mark inverted question mark003-0804 2-3-54 Kikusui 4-jo, Shiroishi-ku, Sapporo, Hokkaido, Japan. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Curr Oncol Rep JT - Current oncology reports JID - 100888967 RN - 0 (Immune Checkpoint Inhibitors) SB - IM MH - Humans MH - *Immune Checkpoint Inhibitors/therapeutic use MH - *Gastrointestinal Neoplasms/drug therapy/microbiology/immunology MH - *Gastrointestinal Microbiome/drug effects/immunology MH - Reproducibility of Results MH - Translational Research, Biomedical OTO - NOTNLM OT - Clinical utility OT - External validation OT - Gastrointestinal cancer OT - Gut microbiome OT - Immune checkpoint inhibitors OT - Metagenomics COIS- Declarations. AI-Assisted Preparation: ChatGPT (OpenAI) assisted with literature organization, language drafting and editorial consistency checking; Claude (Anthropic) supplied figure-development suggestions. The corresponding author reviewed and signed off the 23 prioritized source-to-claim entries in the internal Tier 1 audit, including acceptance of the explicitly unresolved Han cohort attribution. The 37 cited references underwent a separate bibliographic metadata check. These bounded checks do not constitute exhaustive primary-source verification of every statement in the review. The authors retain responsibility for all scientific content, citations, statistical results and final figures; AI assistance does not constitute independent source verification. Competing interests: Tamotsu Sagawa reports lecture honoraria from Ono Pharmaceutical, Bristol Myers Squibb, Astellas Pharma, Eli Lilly Japan, and MSD, and a time-limited advisory/consulting agreement with Takeda Pharmaceutical (July-August 2026; completed). Masahiro Hirakawa, Hiroyuki Nagashima, and Koshi Fujikawa declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:11 PHST- 2026/10/09 00:00 [received] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:11 [entrez] AID - 10.1007/s11912-026-01853-9 [pii] AID - 10.1007/s11912-026-01853-9 [doi] PST - epublish SO - Curr Oncol Rep. 2026 Oct 28;28(1):114. doi: 10.1007/s11912-026-01853-9. PMID- 42955198 OWN - NLM STAT- Publisher LR - 20261029 IS - 2168-4804 (Electronic) IS - 2168-4790 (Linking) DP - 2026 Oct 28 TI - Post-marketing Safety Assessment in the ICH M14 Era: An International Comparison of Real-World Data Utilization Within Risk Management Plans in the European Union, Japan, and the Republic of Korea. LID - 10.1007/s43441-026-01068-x [doi] AB - ICH M14 reached Step 4 in September 2025 and became effective in the European Union (EU) on March 18, 2026. This narrative review compares the institutional pathways and methodological considerations linking real-world data (RWD) studies to risk management plans (RMPs) in the EU, Japan, and the Republic of Korea across eight author-defined domains derived from M14. Regulatory documents, selected EU outcomes of imposed non-interventional post-authorization safety studies (PASS) involving nationally authorized products, and a published review of Japanese post-marketing database studies (PMDS) were synthesized as distinct evidence streams. Regulator-led and academic studies provided separate contextual evidence. EU and Japanese documents link RMP safety concerns to study planning and regulatory reassessment, but differ in the documented arrangements for study-level disclosure and product-level RMP communication. The selected EU cases illustrate different approaches to safety risk assessment and evaluation of prescription restrictions. A Japanese review reported 85 PMDS in 63 of 619 RMPs after deduplication. Korea established database study provisions in 2020 and methodological guidance in 2021 before unified RMP management. The synthesis identified clear safety questions, question-appropriate data and design, and the linkage of the findings to regulatory review and follow-up as practical implementation considerations. Complementary guidance remains relevant for utilization and risk-minimization studies outside M14's direct scope. Differences in sampling frames and study maturity limit quantitative comparisons of uptake and outcomes, and public sources have not established the extent of RWD use within individual Korean RMPs. CI - (c) 2026. The Author(s), under exclusive licence to The Drug Information Association, Inc. FAU - You, Seung-Hun AU - You SH AD - Medical Big Data Research Center, CHA University Bundang Medical Center, Seongnam-si, Republic of Korea. FAU - Kim, Hongsock AU - Kim H AD - Department of Physiology, CHA University School of Medicine, 120, Haeryong-ro, Pocheon-si, Gyeonggi-do, 11160, Republic of Korea. FAU - Yoo, Hyounggyoon AU - Yoo H AD - Department of Pharmacology, CHA University School of Medicine, Seongnam-si, Republic of Korea. AD - Department of Clinical Pharmacology and Therapeutics, CHA University Bundang Medical Center, 59 Yatap-ro, Bundang-gu, Seongnam-si, 13496, Republic of Korea. FAU - Lee, Sung Hwan AU - Lee SH AD - Medical Big Data Research Center, CHA University Bundang Medical Center, Seongnam-si, Republic of Korea. AD - Department of Surgery, CHA University Bundang Medical Center, Seongnam-si, Republic of Korea. FAU - Shin, Wonsuk AU - Shin W AD - Medical Big Data Research Center, CHA University Bundang Medical Center, Seongnam-si, Republic of Korea. wonsug89@chamc.co.kr. AD - Department of Physiology, CHA University School of Medicine, 120, Haeryong-ro, Pocheon-si, Gyeonggi-do, 11160, Republic of Korea. wonsug89@chamc.co.kr. AD - Department of Clinical Pharmacology and Therapeutics, CHA University Bundang Medical Center, 59 Yatap-ro, Bundang-gu, Seongnam-si, 13496, Republic of Korea. wonsug89@chamc.co.kr. FAU - Lee, Yil-Seob AU - Lee YS AD - Department of Clinical Pharmacology and Therapeutics, CHA University Bundang Medical Center, 59 Yatap-ro, Bundang-gu, Seongnam-si, 13496, Republic of Korea. yilseoblee@chamc.co.kr. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - Switzerland TA - Ther Innov Regul Sci JT - Therapeutic innovation & regulatory science JID - 101597411 SB - IM OTO - NOTNLM OT - Non-interventional study OT - Pharmacoepidemiology OT - Pharmacovigilance OT - Post-authorization safety study OT - Post-marketing database study OT - Regulatory harmonization COIS- Declarations. Competing interest: The authors declare no competing interest. Human and Animal Rights: This narrative literature review is based exclusively on publicly available materials. It involved no human subjects or animals, and therefore was not subject to an institutional ethics review. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:11 PHST- 2026/05/21 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:11 [entrez] AID - 10.1007/s43441-026-01068-x [pii] AID - 10.1007/s43441-026-01068-x [doi] PST - aheadofprint SO - Ther Innov Regul Sci. 2026 Oct 28. doi: 10.1007/s43441-026-01068-x. PMID- 42955196 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1559-0100 (Electronic) IS - 1355-008X (Linking) VI - 91 IP - 1 DP - 2026 Oct 29 TI - Automated insulin delivery in older adults with type 1 diabetes: evidence, limitations, and clinical perspectives. LID - 352 [pii] LID - 10.1007/s12020-026-04816-z [doi] AB - OBJECTIVE: Automated insulin delivery (AID) systems have significantly improved the management of type 1 diabetes (T1D), leading to better glycemic control and reduced hypoglycemia. However, older adults (>/= 65 years) remain underrepresented in clinical trials, and evidence specific to this population is limited. DESIGN AND PATIENTS: This narrative review synthesizes current evidence on the efficacy, safety, and usability of AID systems in older adults (>/= 65 years) with T1D. A comprehensive literature search of MEDLINE/PubMed was conducted using the PICO framework to identify relevant RCTs, observational studies, and systematic reviews, supplemented by literature studies on cognitive, functional, and psychosocial factors impacting technology adoption. RESULTS: While data from randomized trials and meta-analyses in the general population consistently demonstrate improvements in time in range (TIR, 70-180 mg/dL) and reductions in hypoglycemia, evidence in older adults is still emerging. Frail individuals and those with multiple comorbidities are often underrepresented or excluded from current studies. Nevertheless, the available evidence suggests that many of these benefits are also maintained in older populations, particularly regarding hypoglycemia reduction. At the same time, factors such as cognitive impairment, functional limitations, caregiver dependence, and reduced digital literacy may affect the adoption and effective use of these technologies. CONCLUSIONS: AID systems appear effective and generally safe in selected older adults, with benefits in glycemic control and hypoglycemia reduction. However, given the marked heterogeneity of this population, further studies are needed to better define their role and applicability in routine clinical practice. CI - (c) 2026. The Author(s). FAU - Galeano, Francesco AU - Galeano F AUID- ORCID: 0009-0004-4104-1105 AD - Department of Clinical and Experimental Medicine, Endocrinology Section, Garibaldi-Nesima Hospital, University of Catania, Catania, 95122, CT, Italy. FAU - Mobilia, Agnese Rita Catena AU - Mobilia ARC AUID- ORCID: 0009-0004-9357-7307 AD - Department of Clinical and Experimental Medicine, Endocrinology Section, Garibaldi-Nesima Hospital, University of Catania, Catania, 95122, CT, Italy. FAU - Fimognari, Filippo Luca AU - Fimognari FL AUID- ORCID: 0000-0002-3623-826X AD - Department of Medicine, Unit of Geriatrics, Azienda Ospedaliera "Annunziata - Mariano Santo - S. Barbara", Cosenza, 87100, CS, Italy. FAU - Oteri, Vittorio AU - Oteri V AUID- ORCID: 0000-0001-5210-8601 AD - Department of Clinical and Experimental Medicine, Endocrinology Section, Garibaldi-Nesima Hospital, University of Catania, Catania, 95122, CT, Italy. research@droteri.it. FAU - Cannarella, Rossella AU - Cannarella R AUID- ORCID: 0000-0003-4599-8487 AD - Department of Clinical and Experimental Medicine, University of Catania, Catania, Italy. FAU - Papa, Giuseppe AU - Papa G AUID- ORCID: 0009-0009-6663-937X AD - Unit of Metabolic and Endocrine Disease, "Centro Catanese di Medicina e Chirurgia" Clinic, Catania, Italy. FAU - Le Moli, Rosario AU - Le Moli R AUID- ORCID: 0000-0002-1398-9271 AD - Department of Medicine and Surgery, University Kore of Enna, Enna, Italy. FAU - Piticchio, Tommaso AU - Piticchio T AUID- ORCID: 0000-0002-0567-6494 AD - Unit of Diabetology, Metabolic and Endocrine Diseases, "Cannizzaro" Emergency Hospital, Catania, Italy. FAU - Di Pino, Antonino AU - Di Pino A AUID- ORCID: 0000-0003-1705-2782 AD - Department of Clinical and Experimental Medicine, Internal Medicine, Garibaldi- Nesima Hospital, University of Catania, Catania, 95122, CT, Italy. FAU - Di Marco, Maurizio AU - Di Marco M AUID- ORCID: 0000-0001-9021-6101 AD - Department of Clinical and Experimental Medicine, Internal Medicine, Garibaldi- Nesima Hospital, University of Catania, Catania, 95122, CT, Italy. FAU - Scilletta, Sabrina AU - Scilletta S AUID- ORCID: 0009-0001-9061-0368 AD - Department of Clinical and Experimental Medicine, Internal Medicine, Garibaldi- Nesima Hospital, University of Catania, Catania, 95122, CT, Italy. FAU - Frasca, Francesco AU - Frasca F AUID- ORCID: 0000-0002-5556-3201 AD - Department of Clinical and Experimental Medicine, Endocrinology Section, Garibaldi-Nesima Hospital, University of Catania, Catania, 95122, CT, Italy. FAU - Tumminia, Andrea AU - Tumminia A AUID- ORCID: 0000-0001-5820-4178 AD - Endocrine Unit, Garibaldi-Nesima Hospital, Catania, 95122, CT, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - United States TA - Endocrine JT - Endocrine JID - 9434444 RN - 0 (Insulin) RN - 0 (Hypoglycemic Agents) SB - IM MH - Humans MH - *Diabetes Mellitus, Type 1/drug therapy/blood MH - *Insulin/administration & dosage/therapeutic use MH - Aged MH - *Insulin Infusion Systems MH - *Hypoglycemic Agents/administration & dosage/therapeutic use MH - Hypoglycemia/prevention & control OTO - NOTNLM OT - Automated insulin delivery OT - Diabetes technology. OT - Hybrid closed-loop OT - Hypoglycemia OT - Older adults OT - Type 1 diabetes COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:11 PHST- 2026/06/21 00:00 [received] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:11 [entrez] AID - 10.1007/s12020-026-04816-z [pii] AID - 10.1007/s12020-026-04816-z [doi] PST - epublish SO - Endocrine. 2026 Oct 29;91(1):352. doi: 10.1007/s12020-026-04816-z. PMID- 42955180 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1941-6636 (Electronic) VI - 57 IP - 1 DP - 2026 Oct 28 TI - Advanced Anal Squamous Cell Carcinoma in the Chemoimmunotherapy Era: Retifanlimab, Nivolumab, and a Practical Framework for Treatment Selection. LID - 247 [pii] LID - 10.1007/s12029-026-01621-9 [doi] AB - Advanced anal squamous cell carcinoma (ASCC) is a rare human papillomavirus-associated malignancy with historically limited prospective evidence for systemic therapy. InterAAct established carboplatin-paclitaxel as a clinically favorable chemotherapy backbone compared with cisplatin-fluorouracil. POD1UM-303/InterAACT-2 subsequently showed that adding retifanlimab significantly improved progression-free survival in patients with no prior systemic therapy for advanced disease. At the final overall-survival analysis, median overall survival was 32.8 versus 22.2 months (HR 0.75, 95% CI 0.55-1.01); the regimen has received regulatory authorization in the United States, the European Union, and Japan. EA2176 is evaluating carboplatin-paclitaxel with or without nivolumab and will clarify whether first-line PD-1-based chemoimmunotherapy represents a reproducible class effect. This focused narrative review critically summarizes the evidence for retifanlimab- and nivolumab-based strategies and proposes a practical framework for treatment selection. Key considerations include performance status, immune-checkpoint-inhibitor eligibility, HIV infection, autoimmune risk, neuropathy, organ function, tumor burden, local symptom control, and post-chemoimmunotherapy sequencing. Retifanlimab plus carboplatin-paclitaxel currently has the most mature phase III evidence, whereas nivolumab-based chemoimmunotherapy remains investigational pending EA2176. Treatment decisions should distinguish phase III-validated options from class-effect extrapolation and integrate multidisciplinary local therapy when appropriate. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Sagawa, Tamotsu AU - Sagawa T AUID- ORCID: 0000-0002-1994-4795 AD - Department of Gastroenterology, National Hospital Organization Hokkaido Cancer Center, 003-0804, 3-54 Kikusui 4-jo 2-chome, Shiroishi-ku, Hokkaido, Sapporo, Japan. stamotsu@jk9.so-net.ne.jp. FAU - Hirakawa, Masahiro AU - Hirakawa M AD - Division of Medical Oncology, Department of Internal Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan. FAU - Nagashima, Hiroyuki AU - Nagashima H AD - Department of Gastroenterology, National Hospital Organization Hokkaido Cancer Center, 003-0804, 3-54 Kikusui 4-jo 2-chome, Shiroishi-ku, Hokkaido, Sapporo, Japan. FAU - Fujikawa, Koshi AU - Fujikawa K AD - Department of Gastroenterology, National Hospital Organization Hokkaido Cancer Center, 003-0804, 3-54 Kikusui 4-jo 2-chome, Shiroishi-ku, Hokkaido, Sapporo, Japan. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - J Gastrointest Cancer JT - Journal of gastrointestinal cancer JID - 101479627 RN - 31YO63LBSN (Nivolumab) RN - 0 (Carbamates) RN - 0 (Immune Checkpoint Inhibitors) SB - IM MH - Humans MH - *Anus Neoplasms/drug therapy/mortality/pathology/immunology MH - *Nivolumab/therapeutic use/pharmacology MH - *Carcinoma, Squamous Cell/drug therapy/mortality/pathology/immunology MH - *Antineoplastic Combined Chemotherapy Protocols/therapeutic use/pharmacology MH - *Carbamates/therapeutic use/pharmacology MH - Immune Checkpoint Inhibitors/therapeutic use OTO - NOTNLM OT - Anal squamous cell carcinoma OT - Chemoimmunotherapy OT - Nivolumab OT - Retifanlimab OT - Treatment selection OT - Treatment sequencing COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:10 PHST- 2026/07/17 00:00 [received] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:10 [entrez] AID - 10.1007/s12029-026-01621-9 [pii] AID - 10.1007/s12029-026-01621-9 [doi] PST - epublish SO - J Gastrointest Cancer. 2026 Oct 28;57(1):247. doi: 10.1007/s12029-026-01621-9. PMID- 42955166 OWN - NLM STAT- Publisher LR - 20261029 IS - 2948-2933 (Electronic) IS - 2948-2925 (Linking) DP - 2026 Oct 29 TI - MSGN: Multi-stage Self-constrained Generative Network for Orthodontic Treatment Prediction from Color Lateral Facial Photographs. LID - 10.1007/s10278-026-02410-3 [doi] AB - People seek orthodontic treatment to improve their facial appearance. An unsolved challenge exists: how patients can visualize their post-treatment appearance before undergoing years of treatment. Existing methods that generate radiographic prediction images are not intuitive for patients. This study presents a multi-stage self-constrained generative network, MSGN, which predicts the post-treatment appearance from a single color lateral facial photograph without X-ray imaging or manual landmark tracing. MSGN detects the orthodontic change region, regresses the individualized tooth correction data, uses these data to guide post-treatment contour generation, performs photorealistic skin synthesis, and blends the result seamlessly with the untreated regions. The model was trained and evaluated on 151 patients who completed fixed-appliance extraction treatment using fivefold cross-validation, so that every patient received an independent out-of-fold prediction. The mean absolute errors were 2.16 mm for the aesthetic-line distance, 7.46 composite function for the nasolabial angle, and 6.58 composite function for the mentolabial angle, with a mean predicted-to-true contour point distance of 2.08 mm; all key metrics are reported with bootstrap 95% confidence intervals. One case is processed in about 2 min on a standard computer. From a patient-centered perspective, MSGN therefore offers a convenient auxiliary reference that visualizes the possible post-treatment appearance to strengthen doctor-patient communication and support shared decision-making, and ongoing multi-center prospective validation will further broaden its clinical role. CI - (c) 2026. The Author(s) under exclusive licence to Society for Imaging Informatics in Medicine. FAU - Song, Bochao AU - Song B AD - The Robotics Institute, School of Mechanical Engineering and Automation, Beihang University, 100191, Beijing, China. FAU - Niu, Qin AU - Niu Q AD - Department of Orthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, China. FAU - Chen, Jiawei AU - Chen J AD - The Robotics Institute, School of Mechanical Engineering and Automation, Beihang University, 100191, Beijing, China. FAU - Zi, Peijin AU - Zi P AD - The Robotics Institute, School of Mechanical Engineering and Automation, Beihang University, 100191, Beijing, China. FAU - Xu, Qinghua AU - Xu Q AD - Ultrasound Medicine Department, China-Japan Friendship Hospital, 100029, Beijing, China. xuqinghua0128@163.com. FAU - Chen, Si AU - Chen S AD - Department of Orthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing, China. elisa02@163.com. FAU - Xu, Kun AU - Xu K AD - The Robotics Institute, School of Mechanical Engineering and Automation, Beihang University, 100191, Beijing, China. LA - eng GR - L232108/Natural Science Foundation of Beijing Municipality/ GR - 62672009/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261029 PL - Switzerland TA - J Imaging Inform Med JT - Journal of imaging informatics in medicine JID - 9918663679206676 SB - IM OTO - NOTNLM OT - Artificial intelligence OT - Doctor-patient communication OT - Medical image generation OT - Orthodontic OT - Treatment prediction COIS- Declarations. Ethics Approval: The study sample was selected from a database of patients who completed orthodontic treatment between 2019 and 2024 at Peking University School and Hospital of Stomatology. All the records were anonymized and de-identified prior to analysis. This retrospective study was approved by the PKUSS Ethics Committee (Approval Number: PKUS $$\lnot $$ not SIRB-202054049). This retrospective study was approved by the Ethics Committee of Peking University School and Hospital of Stomatology (PKUSSIRB, Approval Number: PKUSSIRB-202054049). All patients or their legal guardians received a thorough explanation of the study protocol and provided written informed consent, which explicitly covers the use of their facial photographs for artificial-intelligence model training, research analysis, and academic publication. All records were anonymized and de-identified prior to analysis, and written consent was additionally obtained from the patients whose example images are presented in the figures. The study sample was selected from patients who completed orthodontic treatment between 2019 and 2024 at Peking University School and Hospital of Stomatology. Consent to Participate: Patients undergoing orthodontic treatment and X-ray imaging were provided with a thorough explanation of the protocol for using their radiographic data in scientific research. An informed consent form was obtained from each patient or their legal guardian, authorizing this use. The photos were taken as a routine for orthodontic patients and do not involve any ethical issues. Consent for Publication: Patients undergoing orthodontic treatment and X-ray imaging were provided with a thorough explanation of the protocol for using their radiographic data in scientific research. An informed consent form was obtained from each patient or their legal guardian, authorizing this use. Conflict of Interest: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:10 PHST- 2026/05/30 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:10 [entrez] AID - 10.1007/s10278-026-02410-3 [pii] AID - 10.1007/s10278-026-02410-3 [doi] PST - aheadofprint SO - J Imaging Inform Med. 2026 Oct 29. doi: 10.1007/s10278-026-02410-3. PMID- 42955165 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1863-2491 (Electronic) IS - 1863-2483 (Linking) VI - 20 IP - 1 DP - 2026 Oct 29 TI - Robot-assisted minimally invasive versus conventional open transforaminal lumbar interbody fusion for lumbar degenerative disease: a prospective randomized controlled trial. LID - 1155 [pii] LID - 10.1007/s11701-026-04001-4 [doi] AB - Robotic guidance may improve pedicle screw placement during minimally invasive transforaminal lumbar interbody fusion (MIS-TLIF), but its clinical value remains uncertain. In this prospective, single-center, assessor-blinded trial, 128 patients were randomly assigned in a prespecified 1:3 ratio to robot-assisted MIS-TLIF (n = 32) or conventional fluoroscopy-guided open TLIF (n = 96) using an independent statistician's computer-generated sequence. The primary outcome was the Oswestry Disability Index (ODI) at 2 weeks. Technical, perioperative, and radiographic outcomes were assessed through 18 months; later assessments were exploratory.At 2 weeks, ODI favored robotic assistance (28.25 +/- 6.64 vs 31.52 +/- 6.55; mean difference, -3.27; 95% confidence interval, -5.98 to -0.56; P = 0.019), but was below the 10-point sample-size target and nonsignificant at 3 months. Robotic assistance favored grade A screw placement (98.9% vs 94.5%; P = 0.037) and reduced intraoperative adjustment (2.8% vs 7.3%; P = 0.030); facet joint violation and reoperation did not differ significantly. Operative time was longer, whereas blood loss, ambulation, drain removal, and hospital stay favored robotic assistance. Fusion rates and adjacent-segment disc height were similar. Multifidus cross-sectional area was greater with robotic assistance at 12 and 18 months in patients with evaluable imaging. Robot-assisted MIS-TLIF was associated with earlier recovery, higher screw accuracy, and greater postoperative multifidus cross-sectional area, but longer operative time without demonstrated mid-term clinical or fusion benefits. Larger multicenter trials are warranted. Trial Registration:Chinese Clinical Trial Registry, ChiCTR2400082553. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature. FAU - Wang, Pengru AU - Wang P AD - Department of Orthopedic Oncology, Shanghai Changzheng Hospital, Naval Medical University, 415 Fengyang Road, Shanghai, 200003, China. FAU - Shen, Zhilong AU - Shen Z AD - Department of Orthopedic Oncology, Shanghai Changzheng Hospital, Naval Medical University, 415 Fengyang Road, Shanghai, 200003, China. FAU - Ni, Liangwei AU - Ni L AD - Department of Orthopedic Oncology, Shanghai Changzheng Hospital, Naval Medical University, 415 Fengyang Road, Shanghai, 200003, China. FAU - Xu, Gan AU - Xu G AD - Department of Orthopedic Oncology, Shanghai Changzheng Hospital, Naval Medical University, 415 Fengyang Road, Shanghai, 200003, China. FAU - Yuan, Hao AU - Yuan H AD - Department of Orthopedic Oncology, Shanghai Changzheng Hospital, Naval Medical University, 415 Fengyang Road, Shanghai, 200003, China. FAU - Mao, Yongxin AU - Mao Y AD - Department of Orthopedic Oncology, Shanghai Changzheng Hospital, Naval Medical University, 415 Fengyang Road, Shanghai, 200003, China. FAU - Xiao, Jianru AU - Xiao J AD - Department of Orthopedic Oncology, Shanghai Changzheng Hospital, Naval Medical University, 415 Fengyang Road, Shanghai, 200003, China. jianruxiao83@163.com. FAU - Xu, Wei AU - Xu W AD - Department of Orthopedic Oncology, Shanghai Changzheng Hospital, Naval Medical University, 415 Fengyang Road, Shanghai, 200003, China. xuwei_spine@163.com. LA - eng PT - Comparative Study PT - Journal Article PT - Randomized Controlled Trial DEP - 20261029 PL - England TA - J Robot Surg JT - Journal of robotic surgery JID - 101300401 SB - IM MH - Humans MH - *Spinal Fusion/methods MH - *Robotic Surgical Procedures/methods MH - *Lumbar Vertebrae/surgery MH - Prospective Studies MH - *Minimally Invasive Surgical Procedures/methods MH - Female MH - Male MH - *Intervertebral Disc Degeneration/surgery MH - Middle Aged MH - Treatment Outcome MH - Pedicle Screws MH - Aged MH - Operative Time OTO - NOTNLM OT - Minimally invasive spine surgery OT - Multifidus muscle OT - Pedicle screw accuracy OT - Randomized controlled trial OT - Robot-assisted surgery OT - Transforaminal lumbar interbody fusion COIS- Declarations. Competing interests: The authors have no relevant financial or non-financial interests to disclose. Ethical approval: The study was performed in accordance with the Declaration of Helsinki. Approval was granted by the Shanghai Changzheng Hospital Medical Ethics Committee on September 30, 2022 (approval no. 2022SL058). Consent to participate: Written informed consent was obtained from all individual participants before enrollment. Consent to publish: Not applicable. The manuscript contains no identifiable individual participant data. Trial registration: Chinese Clinical Trial Registry, ChiCTR2400082553; registered April 1, 2024 (retrospectively registered). EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:10 PHST- 2026/07/31 00:00 [received] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:10 [entrez] AID - 10.1007/s11701-026-04001-4 [pii] AID - 10.1007/s11701-026-04001-4 [doi] PST - epublish SO - J Robot Surg. 2026 Oct 29;20(1):1155. doi: 10.1007/s11701-026-04001-4. PMID- 42955162 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1558-822X (Electronic) IS - 1558-8211 (Linking) VI - 21 IP - 1 DP - 2026 Oct 29 TI - The Evolving Landscape of Reduced-Intensity Conditioning: Balancing Efficacy and Toxicity Across Hematologic Malignancies. LID - 23 [pii] LID - 10.1007/s11899-026-00791-4 [doi] AB - PURPOSE: Allogeneic hematopoietic stem cell transplantation (allo-HSCT) has evolved from traditional myeloablative conditioning toward reduced-intensity conditioning (RIC) and strategies using donor-derived immune effector cells. This review summarizes trends in RIC, proposes a practical classification of regimens, and underscores personalized risk stratification and disease-specific conditioning. RECENT FINDINGS: Fludarabine-based backbones combined with alkylating agents-melphalan (Flu-Mel), busulfan (Flu-Bu), or cyclophosphamide (Flu-Cy)-remain widely adopted due to favorable tolerability and antitumor activity. Large CIBMTR analyses indicate melphalan-based RIC confers superior long-term survival in older patients with myeloid malignancies, primarily through reduced relapse rates. The emergence of integrated prognostic tools such as the Hematopoietic Cell Transplant-Composite Risk (HCT-CR) score and the HCT Frailty Scale enhances pretransplant risk assessment and patient selection. Incorporation of measurable residual disease (MRD) testing and next-generation sequencing (NGS) chimerism refines conditioning intensity decisions and enables earlier relapse detection and intervention. While most prospective trials have focused on AML and MDS, accumulating evidence supports RIC efficacy in selected patients with ALL and various lymphomas. Collectively, data emphasize that optimal conditioning is disease-, risk-, and patient-specific, requiring tailored approaches. RIC has extended allo-HSCT to older, frailer patients, achieving outcomes comparable to myeloablative regimens despite relapse risk. Future work should prioritize prospective disease-specific trials comparing RIC regimens; integration of dynamic biomarkers (MRD, immune profiling, NGS chimerism) into adaptive conditioning; development of frailty-adjusted protocols; and evaluation of post-transplant cellular therapies and targeted maintenance to prevent relapse. These advances aim to personalize conditioning and post-transplant management to maximize survival and minimize toxicity. CI - (c) 2026. The Author(s). FAU - Chumnumsiriwath, Piyatida AU - Chumnumsiriwath P AD - Hematopoietic Stem Cell Transplantation and Cellular Therapy Program, Division of Hematology/Oncology, Department of Medicine, University of California Irvine, Orange, CA, 92868, USA. pkongtim@uci.edu. AD - Division of Hematology, Department of Medicine, Faculty of Medicine Naresuan Hospital, Naresuan University, Phitsanulok, Thailand. pkongtim@uci.edu. FAU - Ciurea, Stefan O AU - Ciurea SO AD - Hematopoietic Stem Cell Transplantation and Cellular Therapy Program, Division of Hematology/Oncology, Department of Medicine, University of California Irvine, Orange, CA, 92868, USA. FAU - Kongtim, Piyanuch AU - Kongtim P AD - Hematopoietic Stem Cell Transplantation and Cellular Therapy Program, Division of Hematology/Oncology, Department of Medicine, University of California Irvine, Orange, CA, 92868, USA. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - United States TA - Curr Hematol Malig Rep JT - Current hematologic malignancy reports JID - 101262565 SB - IM MH - Humans MH - *Transplantation Conditioning/methods/adverse effects MH - *Hematologic Neoplasms/therapy/mortality MH - *Hematopoietic Stem Cell Transplantation/methods/adverse effects MH - Treatment Outcome MH - Transplantation, Homologous OTO - NOTNLM OT - Allogeneic stem cell transplantation OT - Graft-versus-tumor effects (GVT) OT - Myeloablative conditioning regimens (MAC) OT - Personalized conditioning regimen OT - Reduced-intensity conditioning regimens (RIC) OT - Risk-adapted tools COIS- Declarations. Ethics Approval and Consent to Participate: This article does not contain any studies with human, or animal subjects performed by any of the authors. Competing Interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:09 PHST- 2026/04/23 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:09 [entrez] AID - 10.1007/s11899-026-00791-4 [pii] AID - 10.1007/s11899-026-00791-4 [doi] PST - epublish SO - Curr Hematol Malig Rep. 2026 Oct 29;21(1):23. doi: 10.1007/s11899-026-00791-4. PMID- 42955158 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1530-9932 (Electronic) IS - 1530-9932 (Linking) VI - 27 IP - 8 DP - 2026 Oct 28 TI - A Heuristic Framework for Comparative Deformation Mapping of Pharmaceutical Excipients Using Compaction Simulator Data. LID - 344 [pii] LID - 10.1208/s12249-026-03577-2 [doi] AB - Understanding the material deformation behavior in tablet dies is crucial for pharmaceutical development, as it directly influences manufacturability and final tablet properties. Heckel analysis provides a theoretical framework for assessing material deformation during compaction, but its direct correlation with real-world tableting behavior remains an area of interest. This study introduces a framework for characterizing the deformation mechanisms of pharmaceutical powders. It addresses limitations in conventional methods that rely solely on yield pressure (Py) and strain rate sensitivity (SRS), which fail to fully differentiate elastic and plastic deformation. The heuristic framework for comparative deformation mapping enables differentiation of excipients across the brittle-plastic-elastic space using two parameters from a compaction simulator: Py (from Heckel analysis) and pressure drop (DeltaP, from an in-die stress relaxation test). DeltaP is treated as a descriptor of time-dependent (viscoplastic and viscoelastic) deformation rather than as a direct measure of elasticity, and its use as an empirical surrogate for elastic character was benchmarked against independent force-displacement elastic energy. Applied to ten common pharmaceutical excipients, the framework differentiated materials that Py and SRS alone did not resolve (SRS was determined for the four reference materials). Across the ten materials, DeltaP tracked elastic energy mainly between excipient classes, whereas within the six lactose grades the relationship was reversed, indicating that DeltaP differences within a class reflect time-dependent deformation. The framework is therefore proposed as a comparative, material-sparing screening tool for early-stage excipient selection, not as a predictor of tableting performance or scale-up behavior. CI - (c) 2026. The Author(s). FAU - Fathollahi, Sara AU - Fathollahi S AUID- ORCID: 0009-0004-0461-2298 AD - DFE Pharma, Klever Strasse 187, 47568, Goch, Germany. sara.fathollahi@dfepharma.com. FAU - Janssen, Pauline H M AU - Janssen PHM AUID- ORCID: 0000-0002-0063-5992 AD - DFE Pharma, Klever Strasse 187, 47568, Goch, Germany. AD - Department of Pharmaceutical Technology and Biopharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV, Groningen, The Netherlands. FAU - Patel, Arpan AU - Patel A AUID- ORCID: 0009-0001-7655-2106 AD - DFE Pharma, Klever Strasse 187, 47568, Goch, Germany. FAU - Peeters, Marie AU - Peeters M AUID- ORCID: 0009-0006-8881-6937 AD - Faculty of Science, Radboud University, Heyendaalseweg 135, 6525 AJ, Nijmegen, Netherlands. FAU - Dickhoff, Bastiaan H J AU - Dickhoff BHJ AUID- ORCID: 0000-0003-3141-302X AD - DFE Pharma, Klever Strasse 187, 47568, Goch, Germany. LA - eng PT - Comparative Study PT - Journal Article DEP - 20261028 PL - United States TA - AAPS PharmSciTech JT - AAPS PharmSciTech JID - 100960111 RN - 0 (Excipients) RN - 0 (Tablets) RN - 0 (Powders) RN - J2B2A4N98G (Lactose) SB - IM MH - *Excipients/chemistry MH - Tablets/chemistry MH - Powders/chemistry MH - Chemistry, Pharmaceutical/methods MH - Elasticity MH - Drug Compounding/methods MH - Pressure MH - Lactose/chemistry MH - Viscosity MH - Technology, Pharmaceutical/methods OTO - NOTNLM OT - compressibility OT - deformation mechanism OT - direct compaction OT - heckel analysis OT - pharmaceutical powder COIS- Declarations. Conflict of interest: S.F., P.J., A.P., and B.D. are employees of DFE Pharma, which manufactures and sells several of the excipients evaluated in this study (Table I). EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:09 PHST- 2026/07/17 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:09 [entrez] AID - 10.1208/s12249-026-03577-2 [pii] AID - 10.1208/s12249-026-03577-2 [doi] PST - epublish SO - AAPS PharmSciTech. 2026 Oct 28;27(8):344. doi: 10.1208/s12249-026-03577-2. PMID- 42955157 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1573-7446 (Electronic) IS - 0165-7380 (Linking) VI - 51 IP - 1 DP - 2026 Oct 29 TI - Rapid test performance for the diagnosis of canine visceral leishmaniasis using blood, serum, and saliva. LID - 24 [pii] LID - 10.1007/s11259-026-11602-9 [doi] AB - A key aspect of controlling canine visceral leishmaniasis (CVL) is the availability of accurate diagnostic tools capable of reaching most of the population. In endemic areas with socially vulnerable populations, facilitating rapid CVL diagnosis by reducing sampling difficulties and diagnostic costs could provide substantial benefits. This study evaluated the performance of the Kalazar Detect Canine Rapid Test for CVL diagnosis using blood, serum, and saliva samples, and compared the results among sample types and with PCR detection in lymph nodes. Samples were collected from 100 dogs. Prevalence detected by the rapid diagnostic tests (RDT) was 21% using blood and serum and 17% using saliva, whereas PCR detected a prevalence of 46%. Agreement between serum and blood-based RDTs was 100%, while comparison between serum/blood and saliva showed a kappa index of 0.87, indicating almost perfect agreement. Using PCR as the reference standard, RDTs on serum and blood showed a sensitivity of 0.39 and specificity of 0.94, whereas saliva-based RDT showed a sensitivity of 0.33 and specificity of 0.96. The low sensitivity of the RDTs currently used by the National Leishmaniasis Program indicate that an additional diagnostic method should be incorporated into CVL surveillance in endemic areas. Nevertheless, our results suggest that saliva may be a suitable sample for the Kalazar Detect Canine Rapid Test, as it is easy to collect, less expensive, and less invasive. To our knowledge, this is the first study to evaluate the performance of this RDT for CVL diagnosis using saliva samples. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature B.V. FAU - Lamattina, Daniela AU - Lamattina D AUID- ORCID: 0000-0002-5926-8234 AD - Instituto Nacional de Medicina Tropical (INMeT), Administracion Nacional de Laboratorios e Institutos de Salud (ANLIS),Ministerio de Salud de la Nacion, Puerto Iguazu, Misiones, Puerto Iguazu, Argentina. daniela.lamattina@gmail.com. AD - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires, Argentina. daniela.lamattina@gmail.com. AD - Instituto de Biologia Subtropical (IBS), CONICET-Universidad Nacional de Misiones (UNaM), Misiones, Puerto Iguazu, Argentina. daniela.lamattina@gmail.com. FAU - Sanchez-Fernandez, Candelaria AU - Sanchez-Fernandez C AD - Instituto Nacional de Medicina Tropical (INMeT), Administracion Nacional de Laboratorios e Institutos de Salud (ANLIS),Ministerio de Salud de la Nacion, Puerto Iguazu, Misiones, Puerto Iguazu, Argentina. FAU - Depasquino, Anibal Fernando AU - Depasquino AF AD - Instituto Nacional de Medicina Tropical (INMeT), Administracion Nacional de Laboratorios e Institutos de Salud (ANLIS),Ministerio de Salud de la Nacion, Puerto Iguazu, Misiones, Puerto Iguazu, Argentina. FAU - Laino, Mariano Alberto AU - Laino MA AD - Instituto de Zoonosis "Luis Pasteur", Ministerio de Salud del Gobierno de la Ciudad Autonoma de Buenos Aires, Ciudad Autonoma de Buenos Aires, Argentina. FAU - Siccardi, Fernando Mario AU - Siccardi FM AD - Instituto de Zoonosis "Luis Pasteur", Ministerio de Salud del Gobierno de la Ciudad Autonoma de Buenos Aires, Ciudad Autonoma de Buenos Aires, Argentina. FAU - Salomon, Oscar Daniel AU - Salomon OD AD - Instituto Nacional de Medicina Tropical (INMeT), Administracion Nacional de Laboratorios e Institutos de Salud (ANLIS),Ministerio de Salud de la Nacion, Puerto Iguazu, Misiones, Puerto Iguazu, Argentina. AD - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires, Argentina. LA - eng GR - FOCANLIS2019-1907/Administracion Nacional de Laboratorios e Institutos de Salud/ GR - PICT2019-2710/Agencia Nacional de Promocion Cientifica y Tecnologica/ PT - Journal Article DEP - 20261029 PL - Switzerland TA - Vet Res Commun JT - Veterinary research communications JID - 8100520 SB - IM MH - Animals MH - Dogs MH - *Leishmaniasis, Visceral/veterinary/diagnosis/blood/epidemiology MH - *Dog Diseases/diagnosis/parasitology/blood/epidemiology MH - *Rapid Diagnostic Tests/veterinary MH - Saliva/parasitology MH - Sensitivity and Specificity MH - Polymerase Chain Reaction/veterinary MH - Female OTO - NOTNLM OT - Leishmania OT - Argentina OT - Neglected tropical disease OT - PCR OT - Rapid diagnostic test OT - Serology COIS- Declarations. Competing interests: The authors declare no competing interests. Animal ethics: Sampling procedures were approved by the Institutional Committee for the Care and Use of Experimental Animals of the National Institute of Agricultural Technology, Santa Fe Regional Center (protocol no. 19-0012). EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:09 PHST- 2026/07/03 00:00 [received] PHST- 2026/10/24 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:09 [entrez] AID - 10.1007/s11259-026-11602-9 [pii] AID - 10.1007/s11259-026-11602-9 [doi] PST - epublish SO - Vet Res Commun. 2026 Oct 29;51(1):24. doi: 10.1007/s11259-026-11602-9. PMID- 42955155 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1678-4405 (Electronic) IS - 1517-8382 (Linking) VI - 57 IP - 1 DP - 2026 Oct 28 TI - Detection of high-risk sequence type ST131 among multidrug-resistant Escherichia coli causing urinary tract infections in Cusco, Peru. LID - 313 [pii] LID - 10.1007/s42770-026-02123-3 [doi] AB - Antimicrobial resistance in Escherichia coli represents a growing public health challenge, particularly in urinary tract infections (UTIs), where this pathogen remains the leading etiological agent. The emergence of multidrug-resistant strains producing extended-spectrum beta-lactamases (ESBLs) and carbapenemases has markedly limited therapeutic options and promoted the persistence of high-risk lineages in hospital settings. In this study, phenotypic, molecular, and genomic characterization were conducted on 50 multidrug-resistant (MDR) E. coli isolates obtained from urine cultures of patients treated at the Regional Hospital of Cusco, Peru. ESBL and carbapenemase production were assessed using standardised phenotypic methods, while resistance genes were detected by endpoint PCR. For genomic characterization, MDR E. coli isolates (RUE 08 and RUE 29) were selected from the panel of 50 isolates. These were chosen as representative samples for whole-genome sequencing and comparative genomic analysis. All isolates were confirmed as ESBL producers, and 40% exhibited carbapenemase production. The detected genes were bla(CTX-M) (94%), bla(TEM) (36%), bla(SHV) (2%), bla(NDM) (26%), bla(OXA-48) (20%) and bla(KPC) was not detected. The detection of bla(NDM) and bla(OXA-48) represents the first documented report of these genes in multidrug-resistant E. coli in the city of Cusco. Genomic analysis identified the high-risk lineage ST131 in one sequenced isolate, characterised by an extensive repertoire of virulence and resistance genes associated with the ExPEC/UPEC pathotype, as well as a second isolate belonging to ST361, which harboured clinically relevant resistance genes but displayed a limited virulence profile. These findings demonstrate the circulation of clinically relevant multidrug-resistant E. coli in southern Peru and underscore the need to strengthen molecular surveillance through the incorporation of genomic tools to enable early detection of high-risk clones and support effective antimicrobial control strategies. CI - (c) 2026. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia. FAU - Huaycho-Guzman, Edgar J AU - Huaycho-Guzman EJ AD - Microbiology and Immunology Research Laboratory, Biological Science Faculty, Universidad Nacional de San Antonio Abad del Cusco, Cusco, Peru. FAU - Quispe-Misme, Joel R AU - Quispe-Misme JR AD - Microbiology and Immunology Research Laboratory, Biological Science Faculty, Universidad Nacional de San Antonio Abad del Cusco, Cusco, Peru. FAU - Quispe-Jurado, Xtopher AU - Quispe-Jurado X AD - Laboratory of Molecular Microbiology and Biotechnology, Biological Science Faculty, Universidad Nacional Mayor de San Marcos, Lima, Peru. FAU - Carrasco-Marin, Marishani AU - Carrasco-Marin M AD - Microbiology and Immunology Research Laboratory, Biological Science Faculty, Universidad Nacional de San Antonio Abad del Cusco, Cusco, Peru. FAU - Espinoza-Culupu, Abraham AU - Espinoza-Culupu A AUID- ORCID: 0000-0002-5553-6385 AD - Laboratory of Molecular Microbiology and Biotechnology, Biological Science Faculty, Universidad Nacional Mayor de San Marcos, Lima, Peru. FAU - Aguilar-Ancori, Elsa Gladys AU - Aguilar-Ancori EG AUID- ORCID: 0000-0002-8942-8868 AD - Microbiology and Immunology Research Laboratory, Biological Science Faculty, Universidad Nacional de San Antonio Abad del Cusco, Cusco, Peru. elsa.aguilar@unsaac.edu.pe. LA - eng GR - No. 001-2021-UNSAAC/Fondo Nacional de Desarrollo Cientifico, Tecnologico y de Innovacion Tecnologica/ GR - FONDECYT/Fondo Nacional de Desarrollo Cientifico, Tecnologico y de Innovacion Tecnologica/ PT - Journal Article DEP - 20261028 PL - Brazil TA - Braz J Microbiol JT - Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] JID - 101095924 RN - EC 3.5.2.6 (beta-Lactamases) RN - 0 (Anti-Bacterial Agents) RN - 0 (Bacterial Proteins) RN - 0 (Escherichia coli Proteins) SB - IM MH - Humans MH - *Urinary Tract Infections/microbiology/epidemiology MH - *Escherichia coli Infections/microbiology/epidemiology MH - *Drug Resistance, Multiple, Bacterial MH - Peru/epidemiology MH - *Escherichia coli/genetics/drug effects/isolation & purification/classification MH - beta-Lactamases/genetics/metabolism MH - Anti-Bacterial Agents/pharmacology MH - Bacterial Proteins/genetics/metabolism MH - Microbial Sensitivity Tests MH - Escherichia coli Proteins/genetics/metabolism OTO - NOTNLM OT - Escherichia coli OT - Carbapenemases OT - Extended-spectrum beta-lactamases (ESBLs) OT - Molecular surveillance OT - Multidrug resistance OT - ST131 COIS- Declarations. Ethics approval: All procedures were performed following the approval of the Institutional Bioethics Committee of Universidad Nacional San Antonio Abad del Cusco with protocol No. CBI-UNSAAC-2022-002 and Universidad Mayor de San Marcos No. 017-2022-CB-FCB-UNMSM. Competing interests: The authors declare that they have no conflicts of interest. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:09 PHST- 2025/12/31 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:09 [entrez] AID - 10.1007/s42770-026-02123-3 [pii] AID - 10.1007/s42770-026-02123-3 [doi] PST - epublish SO - Braz J Microbiol. 2026 Oct 28;57(1):313. doi: 10.1007/s42770-026-02123-3. PMID- 42955154 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1678-8052 (Electronic) IS - 1519-566X (Linking) VI - 55 IP - 1 DP - 2026 Oct 28 TI - First Record of Hesperobaenus rufipes (Coleoptera: Monotomidae) in South America. LID - 97 [pii] LID - 10.1007/s13744-026-01448-w [doi] AB - This study reports the first record of Hesperobaenus rufipes LeConte, 1863 (Coleoptera: Monotomidae) in South America, based on specimens collected in Brazil, specifically in the state of Goias. The beetles were sampled from the soil in soybean and corn fields using pitfall traps. This represents the first record of the species beyond its native range in North America, and its introduction pathway remains unknown. CI - (c) 2026. The Author(s). FAU - de Paula, Flavia Fagundes AU - de Paula FF AUID- ORCID: 0000-0001-9549-9702 AD - Instituto Federal Goiano, Rio Verde, GO, Brazil. flavia.fagundes@ifgoiano.edu.br. FAU - da Silva, Ana Claudia AU - da Silva AC AD - Instituto Federal Goiano, Rio Verde, GO, Brazil. FAU - Barbosa, Jamilly Raniely Batista AU - Barbosa JRB AD - Univ Federal de Goias, Goiania, GO, Brazil. FAU - Sartori, Leonardo Peres AU - Sartori LP AD - Instituto Federal Goiano, Rio Verde, GO, Brazil. FAU - Laumann, Raul Alberto AU - Laumann RA AD - Empresa Brasileira de Pesquisa Agropecuaria, EMBRAPA Recursos Geneticos E Biotecnologia, Brasilia, Federal District, Brazil. FAU - Fuhrmann, Juares AU - Fuhrmann J AD - Instituto Nacional de Coleoptera (INCol), Cuiaba, MT and Museu de Zoologia da Univ de Sao Paulo, Sao Paulo, SP, Brazil. FAU - Fontes, Eliana Maria Gouveia AU - Fontes EMG AD - Empresa Brasileira de Pesquisa Agropecuaria, EMBRAPA Recursos Geneticos E Biotecnologia, Brasilia, Federal District, Brazil. LA - eng GR - 408430/2024-9/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ PT - Journal Article DEP - 20261028 PL - Netherlands TA - Neotrop Entomol JT - Neotropical entomology JID - 101189728 SB - IM MH - Animals MH - *Coleoptera/classification MH - Brazil MH - Glycine max MH - Zea mays OTO - NOTNLM OT - Brazil OT - Cerrado OT - Corn OT - Invasive OT - Soybean COIS- Declarations. Ethical Approval: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:09 PHST- 2026/06/09 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:09 [entrez] AID - 10.1007/s13744-026-01448-w [pii] AID - 10.1007/s13744-026-01448-w [doi] PST - epublish SO - Neotrop Entomol. 2026 Oct 28;55(1):97. doi: 10.1007/s13744-026-01448-w. PMID- 42955146 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1863-2491 (Electronic) IS - 1863-2483 (Linking) VI - 20 IP - 1 DP - 2026 Oct 28 TI - Obesity and perioperative outcomes of robotic-assisted surgery for endometrial cancer: a systematic review and meta-analysis. LID - 1150 [pii] LID - 10.1007/s11701-026-04116-8 [doi] AB - This systematic review and meta-analysis evaluated perioperative outcomes after robotic-assisted surgery for endometrial cancer in obese versus non-obese patients, as classified in the source studies. PubMed, Embase, Web of Science, and the Cochrane Library were searched for comparative studies of robotic-assisted surgery for endometrial cancer. Obesity was defined as BMI >/= 30 kg/m(2) according to each study. The included data were not uniformly restricted to BMI < 40 kg/m(2); consequently, the analysis does not represent class I-II obesity alone. Eight retrospective studies involving 1,879 patients were included. No statistically significant differences were detected in operative time (MD, - 9.02 min; 95% CI, - 21.25 to 3.20; p = 0.15), estimated blood loss (MD, 35.69 mL; 95% CI, - 40.54 to 111.93; p = 0.36), or length of stay (MD, - 0.25 days; 95% CI, - 0.61 to 0.10; p = 0.16). Pooled complication estimates were also nonsignificant. These findings do not establish equivalent safety. In the included retrospective studies, obesity as classified by the original reports was not associated with statistically significant differences in the assessed perioperative outcomes after robotic-assisted surgery. The estimates are imprecise and potentially confounded; they do not establish equivalence or demonstrate superiority over other surgical approaches. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature. FAU - Gong, Jianxue AU - Gong J AD - Department of Obstetrics and Gynecology, The Third Hospital of Shijiazhuang, Shijiazhuang, Hebei, China. 18931882811@163.com. FAU - Zhang, Min AU - Zhang M AD - School of Basic Medical Sciences, Hebei Medical University, Hebei, China. FAU - Chen, Yanrong AU - Chen Y AD - Department of Obstetrics and Gynecology, Beijing Shijitan Hospital Affiliated to Capital Medical University, Beijing, China. FAU - Yang, Mukun AU - Yang M AD - Department of Obstetrics and Gynecology, Beijing Shijitan Hospital Affiliated to Capital Medical University, Beijing, China. FAU - Jia, Xuefang AU - Jia X AD - Department of Ophthalmology, Beijing Aviation General Hospital, Beijing, China. LA - eng PT - Journal Article PT - Meta-Analysis PT - Systematic Review DEP - 20261028 PL - England TA - J Robot Surg JT - Journal of robotic surgery JID - 101300401 SB - IM MH - Humans MH - Female MH - *Endometrial Neoplasms/surgery/complications MH - *Robotic Surgical Procedures/methods/adverse effects MH - *Obesity/complications MH - Treatment Outcome MH - Operative Time MH - Length of Stay MH - Blood Loss, Surgical/statistics & numerical data MH - Postoperative Complications/epidemiology MH - Retrospective Studies OTO - NOTNLM OT - Endometrial cancer OT - Minimally invasive OT - Obesity OT - Robot-assisted surgery COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:08 PHST- 2026/10/01 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:08 [entrez] AID - 10.1007/s11701-026-04116-8 [pii] AID - 10.1007/s11701-026-04116-8 [doi] PST - epublish SO - J Robot Surg. 2026 Oct 28;20(1):1150. doi: 10.1007/s11701-026-04116-8. PMID- 42955143 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1433-8726 (Electronic) IS - 0724-4983 (Linking) VI - 44 IP - 1 DP - 2026 Oct 29 TI - Implementation of a same-session outpatient diode laser ablation pathway for suspected recurrent low-grade NMIBC: feasibility, safety, and early outcomes. LID - 772 [pii] LID - 10.1007/s00345-026-06889-x [doi] AB - PURPOSE: To evaluate feasibility, safety, tolerability, and early outcomes of a same-session "see-and-treat" pathway involving cold-cup biopsy followed by diode laser ablation during surveillance cystoscopy for suspected recurrent low-grade non-muscle-invasive bladder cancer (NMIBC). METHODS: We retrospectively analysed a prospectively maintained single-centre database of patients undergoing outpatient transurethral laser ablation (TULA) during surveillance cystoscopy. Eligible patients had one or two suspected low-grade papillary recurrences /= III events occurred. Biopsy identified pTa low-grade carcinoma in 18 (34.0%), pT0 in 27 (50.9%), carcinoma in situ (CIS) in 4 (7.5%), and intestinal-origin adenocarcinoma in 1 (1.9%). At 3 months, 46/49 (93.9%) had negative cystoscopy; only one had histologically confirmed urothelial recurrence (2.0%). CONCLUSION: Same-session biopsy and diode laser ablation were feasible, safe, and well tolerated in selected patients. Biopsy provided clinically relevant information, including detection of unexpected CIS. Longer follow-up and comparative studies are required. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Ola, Luciano AU - Ola L AUID- ORCID: 0009-0009-8844-4311 AD - Division of Urology, AO Santa Croce e Carle, Cuneo, Italy. ola.l@ospedale.cuneo.it. AD - Division of Urology, AO Santa Croce e Carle, Via Michele Coppino 26, Cuneo, 12100, Italy. ola.l@ospedale.cuneo.it. FAU - Busacca, Giovanni AU - Busacca G AD - Division of Urology, AO Santa Croce e Carle, Cuneo, Italy. FAU - Dalmasso, Ettore AU - Dalmasso E AD - Division of Urology, AO Santa Croce e Carle, Cuneo, Italy. FAU - Ornaghi, Paola Irene AU - Ornaghi PI AD - Division of Urology, AO Santa Croce e Carle, Cuneo, Italy. FAU - Poletti, Filippo AU - Poletti F AD - Division of Urology, AO Santa Croce e Carle, Cuneo, Italy. FAU - Meziere, Juliette AU - Meziere J AD - Division of Urology, AO Santa Croce e Carle, Cuneo, Italy. FAU - Ribolzi, Bianca Sabrina AU - Ribolzi BS AD - Division of Urology, AO Santa Croce e Carle, Cuneo, Italy. AD - Division of Urology, Department of Oncology, AOU San Luigi Gonzaga, Orbassano (To), Italy. FAU - Venezia, Alessandro Enrico Maria AU - Venezia AEM AD - Division of Urology, AO Santa Croce e Carle, Cuneo, Italy. AD - Division of Urology, Department of Oncology, AOU San Luigi Gonzaga, Orbassano (To), Italy. FAU - Dutto, Daniele AU - Dutto D AD - Division of Urology, AO Santa Croce e Carle, Cuneo, Italy. FAU - Morra, Ivano AU - Morra I AD - Division of Urology, AO Santa Croce e Carle, Cuneo, Italy. LA - eng PT - Journal Article DEP - 20261029 PL - Germany TA - World J Urol JT - World journal of urology JID - 8307716 SB - IM MH - Humans MH - *Non-Muscle Invasive Bladder Neoplasms/surgery/pathology MH - Feasibility Studies MH - Female MH - Retrospective Studies MH - *Lasers, Semiconductor/therapeutic use MH - Aged MH - *Neoplasm Recurrence, Local/surgery/pathology MH - Male MH - Treatment Outcome MH - *Laser Therapy/methods MH - Cystoscopy MH - *Ambulatory Surgical Procedures/methods MH - Middle Aged MH - Transurethral Resection of Bladder MH - Biopsy MH - Neoplasm Grading OTO - NOTNLM OT - Ambulatory surgical procedures OT - Cystoscopy OT - Laser therapy OT - Urinary bladder neoplasms COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:08 PHST- 2026/05/17 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:08 [entrez] AID - 10.1007/s00345-026-06889-x [pii] AID - 10.1007/s00345-026-06889-x [doi] PST - epublish SO - World J Urol. 2026 Oct 29;44(1):772. doi: 10.1007/s00345-026-06889-x. PMID- 42955138 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1435-9456 (Electronic) IS - 1435-9448 (Linking) VI - 29 IP - 1 DP - 2026 Oct 12 TI - Fluency and elaboration in dolphin object play: evidence for creativity in behavioral exchanges? LID - 75 [pii] LID - 10.1007/s10071-026-02117-6 [doi] AB - Innovative behaviors in non-human animals have been proposed as indicators of flexible thinking. Although dolphins have demonstrated creativity under stimulus control, these paradigms relied on human-directed tasks and may have overlooked spontaneous expression. This study evaluated whether object play, an unprompted behavior, could serve as a proxy for dolphin creativity by examining object play in bottlenose dolphins (Tursiops truncatus) in managed care and wild Atlantic spotted dolphins (Stenella frontalis). Video data were coded for object play events and analyzed for fluency (behavioral diversity and frequency) and elaboration (sequential organization), with consideration of object type and social context. In managed care, object play differed for bottlenose dolphins significantly by sex and age. Males accounted for 66.2% of events that were dominated by jawing, whereas females exhibited greater behavioral diversity, with higher proportions of carrying (82.4%) and pushing (87.5%). Younger dolphins showed higher frequencies of jawing (62.6%), whereas older individuals exhibited greater use of manipulation behaviors. Object use also differed by sex, with males primarily interacting with a single object category (80.5%), whereas females more frequently engaged with leaves (92.3%) and seagrass (64.3%). In contrast, wild spotted dolphins showed no significant differences across sex or age, and interactions were dominated by seaweed (>/= 81%) across all groups. Lag sequential analysis revealed structured, non-random behavioral organization for OP in both populations. Bottlenose dolphins exhibited more diverse behavior transitions than spotted dolphins. Higher-order analyses identified behavioral associations beyond immediate transitions, supporting behavioral elaboration. Object play offers a window into spontaneous creativity among dolphins to better understand their cognitive capacity in novel situations. CI - (c) 2026. The Author(s). FAU - Adams, Abigail H AU - Adams AH AD - Charismatic Megafauna and Oceanography Laboratory, Nova Southeastern University, Fort Lauderdale, FL, 33004, USA. AD - Dolphin Communication Project, Port Saint Lucie, FL, 34985, USA. FAU - Themelin, Manon AU - Themelin M AD - Dolphin Communication Project, Port Saint Lucie, FL, 34985, USA. FAU - Manitzas Hill, Heather AU - Manitzas Hill H AD - Psychology Department, St. Mary's University, One Camino Santa Maria, San Antonio, TX, 78228, USA. FAU - Yeater, Deirdre B AU - Yeater DB AD - Psychology Department, Sacred Heart University, Fairfield, CT, 06825, USA. FAU - Walker, Rachel T AU - Walker RT AD - Psychology Department, University of the Incarnate Word, San Antonio, TX, 78209, USA. FAU - Hirons, Amy C AU - Hirons AC AD - Charismatic Megafauna and Oceanography Laboratory, Nova Southeastern University, Fort Lauderdale, FL, 33004, USA. FAU - Dudzinski, Kathleen M AU - Dudzinski KM AD - Dolphin Communication Project, Port Saint Lucie, FL, 34985, USA. kathleen@dcpmail.org. LA - eng PT - Journal Article DEP - 20261012 PL - Germany TA - Anim Cogn JT - Animal cognition JID - 9814573 SB - IM MH - Animals MH - Male MH - Female MH - *Creativity MH - *Bottle-Nosed Dolphin/psychology MH - *Play and Playthings/psychology MH - *Behavior, Animal MH - *Stenella/psychology MH - Social Behavior MH - Sex Factors OTO - NOTNLM OT - Atlantic spotted dolphins OT - Bottlenose dolphins OT - Creativity OT - Elaboration OT - Fluency OT - Object play COIS- Declarations. Ethics approval: For this research, data collected at the Bimini, The Bahamas, by DCP researchers was done so under an annual permit from the Bahamian Department of Marine Resources. Data collected at the Roatan Institute for Marine Sciences (RIMS), Anthony's Key Resort, was done so with permission of the RIMS Director under their permit from the Honduras government. All data are observational and collected via a non-invasive protocol. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:08 PHST- 2026/07/16 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:08 [entrez] AID - 10.1007/s10071-026-02117-6 [pii] AID - 10.1007/s10071-026-02117-6 [doi] PST - epublish SO - Anim Cogn. 2026 Oct 12;29(1):75. doi: 10.1007/s10071-026-02117-6. PMID- 42955128 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1590-3478 (Electronic) IS - 1590-1874 (Linking) VI - 47 IP - 11 DP - 2026 Oct 29 TI - How interoception shapes body representations in multiple sclerosis. LID - 896 [pii] LID - 10.1007/s10072-026-09506-2 [doi] AB - BACKGROUND: Both interoception and body representations (BR) are frequently altered in people with multiple sclerosis (PwMS). However, whether distinct dimensions of interoception differentially impact action-oriented (aBR) and non-action-oriented body representations (NaBR) remains unexplored. OBJECTIVE: To characterize the specific associations between objective and subjective dimensions of interoception and BR components in PwMS compared to healthy controls (HC). METHODS: Fifty-nine PwMS and 51 HC completed validated assessments of interoceptive accuracy (IAc), sensibility (ISe), and awareness (IAw). BR was evaluated using the Hand Laterality Task (HLT) for aBR and the Frontal Body Evocation (FBE) Task for NaBR. Correlation and regression analyses were performed with Bonferroni correction. RESULTS: After strict multiple-comparison correction, higher interoceptive sensibility (SAQ Total Score) significantly predicted poorer accuracy on the HLT in PwMS. No significant correlations survived correction in the HC group. Exploratory analyses in PwMS suggested an uncorrected disease-specific link between higher IAc and higher deviation and shorter response times in the FBE task. CONCLUSION: Distinct dimensions of interoception are differentially linked to specific BR components in MS. Subjective body-signal monitoring selectively impairs action-oriented body schema functioning, supporting a multidimensional model of bodily dysfunction. CI - (c) 2026. Fondazione Societa Italiana di Neurologia. FAU - Gaita, Mariachiara AU - Gaita M AD - Department of Medical and Surgical Sciences, 'Magna Graecia' University of Catanzaro, Viale Europa, Catanzaro, 88100, Italy. FAU - Satriano, Federica AU - Satriano F AD - Department of Human Neuroscience, 'Sapienza' University of Rome, Roma, Italy. FAU - Signoriello, Elisabetta AU - Signoriello E AD - UOSD Second Neurology, Multiple Sclerosis Center, University of Campania 'Luigi Vanvitelli', Napoli, Italy. FAU - Lus, Giacomo AU - Lus G AD - UOSD Second Neurology, Multiple Sclerosis Center, University of Campania 'Luigi Vanvitelli', Napoli, Italy. FAU - Cropano, Maria AU - Cropano M AD - UOSD Second Neurology, Multiple Sclerosis Center, University of Campania 'Luigi Vanvitelli', Napoli, Italy. AD - Department of Health Sciences, 'Magna Graecia' University of Catanzaro, Catanzaro, Italy. FAU - Conte, Antonella AU - Conte A AD - Department of Human Neuroscience, 'Sapienza' University of Rome, Roma, Italy. AD - IRCCS Neuromed, Pozzilli, IS, Italy. FAU - Palermo, Liana AU - Palermo L AD - Department of Medical and Surgical Sciences, 'Magna Graecia' University of Catanzaro, Viale Europa, Catanzaro, 88100, Italy. FAU - Ferrazzano, Gina AU - Ferrazzano G AD - Department of Human Neuroscience, 'Sapienza' University of Rome, Roma, Italy. FAU - Raimo, Simona AU - Raimo S AUID- ORCID: 0000-0002-0687-9656 AD - Department of Medical and Surgical Sciences, 'Magna Graecia' University of Catanzaro, Viale Europa, Catanzaro, 88100, Italy. simona.raimo@unicz.it. LA - eng PT - Journal Article DEP - 20261029 PL - Italy TA - Neurol Sci JT - Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology JID - 100959175 SB - IM MH - Humans MH - *Interoception/physiology MH - Female MH - *Multiple Sclerosis/psychology/physiopathology MH - Male MH - *Body Image/psychology MH - Adult MH - Middle Aged MH - Awareness/physiology OTO - NOTNLM OT - Action-oriented body representation OT - Body schema OT - Interoception OT - Multiple sclerosis OT - Multisensory integration COIS- Declarations. Ethical Standards: All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The study was approved by the local ethics committee. Consent for publication: Not applicable. No individual identifiable data are included in this article. Consent to participate: Written informed consent was obtained from all participants prior to study inclusion. Conflict of interest: The authors state that there is no conflict of interest. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:07 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:07 [entrez] AID - 10.1007/s10072-026-09506-2 [pii] AID - 10.1007/s10072-026-09506-2 [doi] PST - epublish SO - Neurol Sci. 2026 Oct 29;47(11):896. doi: 10.1007/s10072-026-09506-2. PMID- 42955114 OWN - NLM STAT- Publisher LR - 20261029 IS - 1432-0932 (Electronic) IS - 0940-6719 (Linking) DP - 2026 Oct 29 TI - Home-based halo gravity traction for severe pediatric spinal deformities using a novel protocol. LID - 10.1007/s00586-026-10471-9 [doi] AB - PURPOSE: We aimed to evaluate the safety and efficacy of an original structured protocol for preoperative home-based halo gravity traction (HGT) in children with severe spinal deformity. METHODS: A treatment protocol for preoperative home-based HGT was developed for children with severe spinal deformity scheduled for posterior spinal fusion. The protocol comprises two phases: Phase 1 (hospital phase, 3-5 days), which includes halo placement, and Phase 2 (home phase), which involves a gradual increase in traction up to 50% of body weight, with weekly clinical, neurological, nutritional, respiratory, and radiographic assessments. RESULTS: We prospectively evaluated 43 consecutive patients; 40 completed the home-based HGT protocol and were included in the analysis, while three were excluded because hospitalization was required due to traction system failure or need for clinical optimization. The mean age at HGT initiation was 12 years +/- 2 years (5.5-18), and 67.5% were female. The mean pre-HGT scoliosis angle was 106 degrees (90-144 degrees ), with a mean angular correction of 25 degrees (23.6%). The mean pre-HGT kyphosis angle was 64 degrees (38-81 degrees ), with a mean angular correction of 23 degrees (36%). The mean duration of HGT was 14.7 weeks (4-57). Complications occurred in six patients (15%). Most patients were able to maintain their usual educational and social activities in their home communities, and families continued their work routines. These results were compared with findings from previously published studies. The mean treatment cost per patient was 6.5-fold higher for inpatient than for home-based treatment. CONCLUSIONS: The home-based HGT protocol achieved results comparable to those reported for hospital-based traction in the literature. Home-based HGT may represent a viable option in carefully selected patients with adequate family support and the ability to adhere to treatment and follow-up requirements. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Melgarejo, Jose Luis AU - Melgarejo JL AD - Garrahan Hospital, Buenos Aires, Argentina. FAU - Piantoni, Lucas AU - Piantoni L AD - Garrahan Hospital, Buenos Aires, Argentina. lucaspiantoni@gmail.com. FAU - Tello, Carlos A AU - Tello CA AD - Garrahan Hospital, Buenos Aires, Argentina. FAU - Eduardo, Galaretto AU - Eduardo G AD - Garrahan Hospital, Buenos Aires, Argentina. FAU - Remondino, Rodrigo AU - Remondino R AD - Garrahan Hospital, Buenos Aires, Argentina. LA - eng PT - Journal Article DEP - 20261029 PL - Germany TA - Eur Spine J JT - European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society JID - 9301980 SB - IM OTO - NOTNLM OT - Halo gravity traction OT - Pediatric OT - Scoliosis OT - Spinal surgery COIS- Declarations. Competing interests: The authors declare no competing interests. Ethics approval and consent to participate: Approval was obtained from the hospital's Institutional Review Board to conduct this study (IRB No. 175/22), and informed consent for participation in the protocol was obtained from the patients' parents or legal guardians, with patient assent obtained when appropriate. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:06 PHST- 2026/04/29 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:06 [entrez] AID - 10.1007/s00586-026-10471-9 [pii] AID - 10.1007/s00586-026-10471-9 [doi] PST - aheadofprint SO - Eur Spine J. 2026 Oct 29. doi: 10.1007/s00586-026-10471-9. PMID- 42955109 OWN - NLM STAT- Publisher LR - 20261029 IS - 1432-1084 (Electronic) IS - 0938-7994 (Linking) DP - 2026 Oct 28 TI - What an unreleased AI model should teach radiology. LID - 10.1007/s00330-026-12909-6 [doi] FAU - Nawabi, Jawed AU - Nawabi J AUID- ORCID: 0000-0002-1137-0643 AD - Department of Neuroradiology, Charite-Universitatsmedizin Berlin, Corporate Member of Freie Universitat Berlin and Humboldt Universitat zu Berlin, Berlin, Germany. jawed.nawabi@charite.de. FAU - Akinci D'Antonoli, Tugba AU - Akinci D'Antonoli T AD - Division of Diagnostic and Interventional Neuroradiology, Department of Radiology, University Hospital Basel, Basel, Switzerland. AD - Department of Pediatric Radiology, University Children's Hospital Basel, Basel, Switzerland. LA - eng PT - Letter DEP - 20261028 PL - Germany TA - Eur Radiol JT - European radiology JID - 9114774 SB - IM COIS- Compliance with ethical standards. Guarantor: The scientific guarantor of this publication is Jawed Nawabi. Conflict of interest: J. Nawabi reports research collaboration agreements with Briya Labs Ltd., Cercare Medical, and Mediaire GmbH, and has received grant funding from the Berlin Institute of Health (BIH) and the European Union (COMFORT; project number: 101079894). Tugba Akinci D'Antonoli is an Editorial Board Member of European Radiology, an Editorial Board Member of Diagnostic and Interventional Radiology, an Associate Editor of Radiology: Artificial Intelligence, and a Board Member and Chair of the Education Committee of the European Society of Medical Imaging Informatics. As such, they did not participate in the selection or review processes for this article. Statistics and biometry: No complex statistical methods were necessary for this paper. Informed consent: n/a. Ethical approval: n/a. Study subjects or cohorts overlap: n/a. Methodology: Opinion EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:05 PHST- 2026/07/05 00:00 [received] PHST- 2026/09/02 00:00 [accepted] PHST- 2026/08/05 00:00 [revised] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:05 [entrez] AID - 10.1007/s00330-026-12909-6 [pii] AID - 10.1007/s00330-026-12909-6 [doi] PST - aheadofprint SO - Eur Radiol. 2026 Oct 28. doi: 10.1007/s00330-026-12909-6. PMID- 42955105 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1437-9813 (Electronic) IS - 0179-0358 (Linking) VI - 42 IP - 1 DP - 2026 Oct 28 TI - Trends in adoption of laparoscopy for the Ladd's procedure: a multicenter analysis. LID - 465 [pii] LID - 10.1007/s00383-026-06667-1 [doi] AB - PURPOSE: We sought to evaluate contemporary trends in utilization of laparoscopy for the Ladd's procedure across 10 children's hospitals. METHODS: Pediatric patients /= 80%) participation. Twelve-month ANCOVA included 331 patients without recorded death, 50 imputations for nondeath missing outcomes, and 16-17 covariates and Benjamini-Hochberg correction across 18 contrasts. The service-recorded WHOQOL-BREF aggregate was exploratory because its scoring algorithm could not be verified. Progression-specific pattern-mixture analyses assessed missingness assumptions. RESULTS: Compared with low participation, moderate and high participation were associated with lower PHQ-9 scores (beta = - 2.64; 95% CI - 3.36 to - 1.92 and - 3.73; - 4.49 to - 2.98, respectively), lower PSQI (beta = - 1.99 and - 1.09) and distress scores (- 0.53 and - 0.87), and higher exploratory WHOQOL-BREF aggregates (4.69 and 3.45); all q < 0.05. High participation was also associated with lower GAD-7 (- 1.14; - 1.84 to - 0.43). Direct high-moderate comparisons favored high participation for depression and anxiety and moderate participation for sleep. FACT-Hep scores were lower with both moderate (- 8.70; - 11.68 to - 5.73) and high participation (- 8.93; - 12.31 to - 5.55). CONCLUSION: Participation showed outcome-specific associations, with more favorable psychological scores alongside poorer disease-specific quality of life. Clinical significance remains uncertain. Selection, residual confounding, cumulative exposure timing, and informative attrition limit interpretation; the findings do not establish an optimal participation level. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Jiang, Feng AU - Jiang F AD - Shengli Clinical Medical College, Fujian Medical University, Fuzhou, 350001, Fujian, China. AD - Center for Experimental Research in Clinical Medicine, Fujian Provincial Hospital, Fuzhou, 350001, Fujian, China. AD - Fuzhou University Affiliated Provincial Hospital, Fuzhou, 350001, Fujian, China. AD - Department of Pain Management, and Vascular Surgery & Interventional Oncology Center, Fujian Provincial Hospital, Fuzhou, 350001, Fujian, China. FAU - Liang, Xiaoqin AU - Liang X AD - Shengli Clinical Medical College, Fujian Medical University, Fuzhou, 350001, Fujian, China. AD - Center for Experimental Research in Clinical Medicine, Fujian Provincial Hospital, Fuzhou, 350001, Fujian, China. AD - Fuzhou University Affiliated Provincial Hospital, Fuzhou, 350001, Fujian, China. AD - Department of Pain Management, and Vascular Surgery & Interventional Oncology Center, Fujian Provincial Hospital, Fuzhou, 350001, Fujian, China. FAU - Jin, Shuang AU - Jin S AD - Shengli Clinical Medical College, Fujian Medical University, Fuzhou, 350001, Fujian, China. AD - Center for Experimental Research in Clinical Medicine, Fujian Provincial Hospital, Fuzhou, 350001, Fujian, China. AD - Fuzhou University Affiliated Provincial Hospital, Fuzhou, 350001, Fujian, China. AD - Department of Nursing, Fujian Provincial Hospital, Fuzhou, 350001, Fujian, China. FAU - Li, Yan AU - Li Y AD - Department of Pathology, Fujian Cancer Hospital, Fuzhou, 350014, Fujian, China. FAU - Cai, Tianhong AU - Cai T AD - Shengli Clinical Medical College, Fujian Medical University, Fuzhou, 350001, Fujian, China. AD - Center for Experimental Research in Clinical Medicine, Fujian Provincial Hospital, Fuzhou, 350001, Fujian, China. AD - Fuzhou University Affiliated Provincial Hospital, Fuzhou, 350001, Fujian, China. FAU - Tang, Haijun AU - Tang H AD - Shengli Clinical Medical College, Fujian Medical University, Fuzhou, 350001, Fujian, China. tanghaijun16@163.com. AD - Center for Experimental Research in Clinical Medicine, Fujian Provincial Hospital, Fuzhou, 350001, Fujian, China. tanghaijun16@163.com. AD - Fuzhou University Affiliated Provincial Hospital, Fuzhou, 350001, Fujian, China. tanghaijun16@163.com. LA - eng GR - 2023Y9324/Joint Fund of Science and Technology Innovation of Fujian Province/ GR - 2024J011648/Natural Science Foundation of Fujian Province/ GR - 2024GGA001/Fujian Province Health Care Young and Middle-aged Backbone Talents Training Project/ PT - Journal Article PT - Multicenter Study DEP - 20261028 PL - Germany TA - Support Care Cancer JT - Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer JID - 9302957 SB - IM MH - Humans MH - Retrospective Studies MH - Female MH - *Liver Neoplasms/psychology/therapy MH - *Carcinoma, Hepatocellular/psychology/therapy MH - Middle Aged MH - *Counseling/methods MH - Male MH - Telemedicine MH - Quality of Life MH - Aged MH - China MH - Depression OTO - NOTNLM OT - Hepatocellular carcinoma OT - Integrated telemedicine OT - Quality of life COIS- Declarations. Ethical approval: Medical Ethics Committee of Fujian Provincial Hospital (KY2026-02-046), formally covering retrospective use of data from Fujian Provincial Hospital and Fujian Cancer Hospital. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:05 PHST- 2026/05/19 00:00 [received] PHST- 2026/10/24 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:05 [entrez] AID - 10.1007/s00520-026-11431-1 [pii] AID - 10.1007/s00520-026-11431-1 [doi] PST - epublish SO - Support Care Cancer. 2026 Oct 28;34(11):1163. doi: 10.1007/s00520-026-11431-1. PMID- 42955097 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1433-7339 (Electronic) IS - 0941-4355 (Linking) VI - 34 IP - 11 DP - 2026 Oct 29 TI - Detecting spiritual pain in electronic medical records using large language models. LID - 1167 [pii] LID - 10.1007/s00520-026-11416-0 [doi] AB - PURPOSE: Spiritual pain is one dimension of total pain in terminally ill patients with cancer. Reducing the burden of assessing spiritual pain in electronic medical records is essential for improving the quality of spiritual care. Large language models may offer a solution. This study explored whether large language models can be used to develop an automatic system for accurately identifying spiritual pain in patients with terminal cancer. METHODS: We used unstructured nursing records from patients with cancer who died in an inpatient palliative care unit at a university hospital in Japan between July and December 2024. A total of 500 records generated within 3 weeks before patients' death were randomly selected for analysis. Records were labeled for the presence of spiritual pain. Gemini-2.5-flash and Microsoft Copilot, accessible within the Tohoku University network environment, were utilized. Prompts included definitions of spiritual pain and example sentences. RESULTS: The analyzed records were derived from 109 patients with cancer (57 (52.3%) male; mean age, 70.9 years). Manual review identified spiritual pain in 56 records (11.2%). Sensitivity for Gemini and Copilot was 1.00; specificity was 0.75 and 0.61; positive predictive value was 0.34 and 0.25; and negative predictive value was 1.00 and 1.00, respectively. Error analysis of false positives indicated that the models frequently misclassified decreased physical functions and delirium as spiritual pain. CONCLUSION: This study demonstrates that large language models can identify spiritual pain with performance comparable to, or exceeding, that of previous studies utilizing classical natural language processing methods. CI - (c) 2026. The Author(s). FAU - Masukawa, Kento AU - Masukawa K AUID- ORCID: 0000-0002-5993-0822 AD - Department of Palliative Nursing, Health Sciences, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan. kento.masukawa.d6@tohoku.ac.jp. FAU - Meguro, Momoe AU - Meguro M AD - Department of Palliative Nursing, Health Sciences, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan. FAU - Aihara, Erika AU - Aihara E AD - Department of Palliative Nursing, Health Sciences, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan. FAU - Ishida, Miku AU - Ishida M AD - Department of Palliative Nursing, Health Sciences, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan. FAU - Kondo, Uta AU - Kondo U AD - Department of Palliative Nursing, Health Sciences, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan. FAU - Inoue, Ryusuke AU - Inoue R AD - Medical Information Technology Center, Tohoku University Hospital, Sendai, Miyagi, Japan. FAU - Nakayama, Masaharu AU - Nakayama M AD - Department of Medical Informatics, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan. FAU - Miyashita, Mitsunori AU - Miyashita M AD - Department of Palliative Nursing, Health Sciences, Tohoku University Graduate School of Medicine, Sendai, Miyagi, Japan. LA - eng GR - 23K18385/Japan Society for the Promotion of Science/ PT - Journal Article DEP - 20261029 PL - Germany TA - Support Care Cancer JT - Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer JID - 9302957 SB - IM MH - Humans MH - *Electronic Health Records/statistics & numerical data MH - *Large Language Models MH - Aged MH - Female MH - Male MH - *Neoplasms/psychology MH - *Spirituality MH - Japan MH - Middle Aged MH - Aged, 80 and over MH - Palliative Care/methods MH - Sensitivity and Specificity MH - *Pain/diagnosis MH - Natural Language Processing OTO - NOTNLM OT - Electronic medical records OT - Large language models OT - Natural language processing OT - Spiritual pain COIS- Declarations. Ethics approval: This study was approved by the Tohoku University Institutional Review Board (Approval No. 2024-1-1006) and was conducted in accordance with the Declaration of Helsinki. Consent to participate: Informed consent was obtained in the form of opt-out on the hospital website. Consent for publication: Not applicable. Conflict of interest: The authors declare no competing interests. Clinical trial number: Not applicable. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:05 PHST- 2026/03/02 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:05 [entrez] AID - 10.1007/s00520-026-11416-0 [pii] AID - 10.1007/s00520-026-11416-0 [doi] PST - epublish SO - Support Care Cancer. 2026 Oct 29;34(11):1167. doi: 10.1007/s00520-026-11416-0. PMID- 42955093 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1436-6215 (Electronic) IS - 1436-6207 (Linking) VI - 65 IP - 8 DP - 2026 Oct 28 TI - Plant-based meat and dairy alternatives in Norway: consumption, nutritional quality and Nutri-Score based on data from the Norkost 4 survey. LID - 288 [pii] LID - 10.1007/s00394-026-04144-y [doi] AB - PURPOSE: Alternatives to animal-sourced foods (Alt-ASFs) are processed plant-based products that mimic animal-sourced foods. This study aimed to describe the consumption of Alt-ASFs in the Norwegian dietary survey (Norkost 4) and compare population characteristics and nutritional intake in regular consumers. Further, to compare the nutritional quality of Alt-ASFs consumed and describe the distribution of the Nutri-Score and NewTools-score - a proposed revised version of Nutri-Score, among these products. METHODS: Dietary data were collected from 1,815 adults in Norkost 4 using 2*24-h recalls and a food frequency questionnaire. Alt-ASFs were identified and compared with corresponding ASFs. Nutritional quality was assessed and products were scored using Nutri-Score and NewTools-score. RESULTS: Overall, the consumption of Alt-ASF was low (mean: 0.3 energy %, SD: 1.6). 8% (n = 147) reported consuming one or more Alt-ASFs regularly, defined as >/= 2 times/week, with dairy alternatives being most frequently consumed. Regular consumers were typically younger, female, and more likely to report a vegetarian diet. Most Alt-ASFs contained lower levels of the assessed micronutrients except for meat and fortified milk, whose micronutrient content was similar or higher than ASFs. There was a wide variation in nutritional scores for Alt-ASFs. Unfortified Alt-ASFs typically had a low micronutrient content but could still achieve favourable Nutri-Scores if low in salt, sugar and saturated fat. CONCLUSION: Our findings indicate that only a minor part of Norwegian adults regularly consumes Alt-ASFs and consumption is low. Shifting from ASFs to Alt-ASFs may have some nutritional benefits and some disadvantages, largely dependent on the chosen products. CI - (c) 2026. The Author(s). FAU - Amberntsson, Anna AU - Amberntsson A AD - Department of Food Safety, Norwegian Institute of Public Health, Oslo, Norway. AD - Centre for Sustainable Diets, Norwegian Institute of Public Health, Oslo, Norway. FAU - Konglevoll, Dina Moxness AU - Konglevoll DM AD - Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway. d.m.konglevoll@medisin.uio.no. FAU - Eliassen, Annika AU - Eliassen A AD - Faculty of Biosciences, Norwegian University of Life Science, As, Norway. FAU - Paulsen, Mari Mohn AU - Paulsen MM AD - Department of Food Safety, Norwegian Institute of Public Health, Oslo, Norway. AD - Centre for Sustainable Diets, Norwegian Institute of Public Health, Oslo, Norway. FAU - Brantsaeter, Anne Lise AU - Brantsaeter AL AD - Department of Food Safety, Norwegian Institute of Public Health, Oslo, Norway. AD - Centre for Sustainable Diets, Norwegian Institute of Public Health, Oslo, Norway. FAU - Abel, Marianne Hope AU - Abel MH AD - Centre for Sustainable Diets, Norwegian Institute of Public Health, Oslo, Norway. AD - Centre for Evaluation of Public Health Measures, Norwegian Institute of Public Health, Oslo, Norway. AD - Department of Physical Health and Ageing, Norwegian Institute of Public Health, Oslo, Norway. FAU - Andersen, Lene Frost AU - Andersen LF AD - Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway. LA - eng PT - Journal Article DEP - 20261028 PL - Germany TA - Eur J Nutr JT - European journal of nutrition JID - 100888704 SB - IM MH - Humans MH - Norway MH - Female MH - *Nutritive Value MH - Adult MH - Male MH - Meat Substitutes MH - *Dairy Products/statistics & numerical data MH - Middle Aged MH - *Diet Surveys MH - Diet, Vegetarian/statistics & numerical data MH - *Diet/methods/statistics & numerical data MH - Animals MH - Young Adult MH - Diet, Plant-Based OTO - NOTNLM OT - Food Rating system OT - Meat and dairy replacement OT - Nutri-Score OT - Nutrient profiling OT - Nutritional quality OT - Plant-based COIS- Declarations. Ethics approval and consent to participate: This study was conducted according to the guidelines laid down in the Declaration of Helsinki. The project was considered not subject to submission by the Regional Committees for Medical and Health Research Ethics (REK) (application nr. 469437) and subsequently reported to Sikt - the Norwegian Agency for Shared Services in Education and Research (ref. 265058). The extraction from the population register was approved by the Norwegian Tax Administration (ref. 2022/5665038), while the Common Contact Register approved the retrieval of mobile phone numbers for the selected sample. The project was also approved by the data protection officer at the UiO. Written or verbal informed consent was obtained from all participants. Conflict of interest: The authors have no conflict of interest to disclose. Competing interests: The authors declare no competing interests. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 12:04 PHST- 2026/04/03 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:04 [entrez] AID - 10.1007/s00394-026-04144-y [pii] AID - 10.1007/s00394-026-04144-y [doi] PST - epublish SO - Eur J Nutr. 2026 Oct 28;65(8):288. doi: 10.1007/s00394-026-04144-y. PMID- 42955088 OWN - NLM STAT- Publisher LR - 20261029 IS - 1558-8238 (Electronic) IS - 0021-9738 (Linking) DP - 2026 Oct 29 TI - HIV reservoir cells do not show general resistance to effector molecules secreted by cytolytic T lymphocytes. LID - e207260 [pii] LID - 10.1172/JCI207260 [doi] AB - To eliminate the persistent reservoir of cells harboring intact HIV-1 proviruses in people living with HIV-1 (PLWH), cure strategies like the Shock-and-Kill approach rely on effector functions of cytolytic T lymphocytes (CTL). CTL are involved in the initial control of HIV-1 viremia and target productively infected cells throughout the course of infection. However, selective killing of susceptible cells could generate a reservoir dominated by cells with dysregulated cell death pathways or other features conferring resistance to killing. Here, we use CTL-engaging single-chain diabodies to assess the rate of lysis of uninfected and HIV-1-infected primary CD4+ T cells under identical CTL pressure in the settings of both latent and active infection. Our findings indicate that with this mode of CTL triggering, infected and uninfected CD4+ T cells from PLWH on ART are generally lysed at identical rates, and that an apparent survival advantage for actively infected CD4+ T cells primarily reflects the reduced surface antigen availability through previously described Nef-dependent downregulation of MHC class I molecules. No survival advantage is observed when the CTL response is directed through diabodies to the stably expressed non-classical MHC class I molecule HLA-E, indicating equal susceptibility to cell death. FAU - Bachmann, Niklas AU - Bachmann N AD - Johns Hopkins University School of Medicine, Baltimore, United States of America. FAU - Kim, Bailey AU - Kim B AD - Johns Hopkins University, Krieger School of Arts and Sciences, Baltimore, United States of America. FAU - Simonetti, Francesco R AU - Simonetti FR AD - Johns Hopkins University School of Medicine, Baltimore, United States of America. FAU - Kovacs, Colin M AU - Kovacs CM AD - Maple Leaf Medical Clinic, Toronto, Canada. FAU - Hoh, Rebecca AU - Hoh R AD - UCSF, San Francisco, United States of America. FAU - Deeks, Steven G AU - Deeks SG AD - UCSF, San Francisco, United States of America. FAU - Siliciano, Janet D AU - Siliciano JD AD - Johns Hopkins University School of Medicine, Baltimore, United States of America. FAU - Siliciano, Robert F AU - Siliciano RF AD - Johns Hopkins University School of Medicine, Baltimore, United States of America. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Clin Invest JT - The Journal of clinical investigation JID - 7802877 SB - IM OTO - NOTNLM OT - AIDS/HIV OT - Apoptosis OT - Immunology OT - Immunotherapy OT - T cells EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 12:03 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 12:03 [entrez] AID - 207260 [pii] AID - 10.1172/JCI207260 [doi] PST - aheadofprint SO - J Clin Invest. 2026 Oct 29:e207260. doi: 10.1172/JCI207260. PMID- 42955072 OWN - NLM STAT- Publisher LR - 20261029 IS - 1938-291X (Electronic) IS - 0022-0493 (Linking) DP - 2026 Oct 29 TI - Quantitative genetic analysis and risk assessment of field-evolved emamectin benzoate resistance in Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae). LID - toag328 [pii] LID - 10.1093/jee/toag328 [doi] AB - A population of Spodoptera litura originating from table grapes in northwest Western Australia in 2024 had 55.1% survival on a discriminating dose of emamectin benzoate in the F1 generation. Survival was stable in this strain (BM6xTG) for the next 7 generations under laboratory selection at the discriminating dose, with 28.9%, 19.6%, and 21.8% mortality in the G4, G6, and G7, respectively. A series of iso-female lines were established from G8 adults and used to generate proxy F2 recombinants (where parental G7 moths were considered as the F0 generation), which were then screened with the discriminating dose of emamectin benzoate. Three lines exhibited 100% survival and were presumed homozygous for resistance. Subsequent quantitative genetic analysis showed that resistance was autosomal, incompletely dominant (DLC = 0.85), and conferred by 1 or a small number of closely linked loci. While there was no major cross-resistance to chlorantraniliprole, indoxacarb, methoxyfenozide, or Vip3A in the BM6xTG strain, it was significantly less sensitive to spinetoram than the laboratory strain. There was a lack of resistance suppression by metabolic inhibitors triphenyl phosphate, diethyl maleate, and piperonyl butoxide, suggesting that metabolic detoxification may not play a role in resistance. A fourth iso-female line was completely insensitive to both emamectin benzoate and chlorantraniliprole, and a complementation test for allelism confirmed multi-resistance to Group 28 and Group 6 insecticides in this strain. The risk that these resistance variants pose to management of S. litura is discussed. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Bird, Lisa J AU - Bird LJ AUID- ORCID: 0000-0003-2669-0540 AD - New South Wales Department of Primary Industries and Regional Development, Tamworth Agricultural Institute, Calala, NSW, Australia. FAU - Dunn, Nicole M AU - Dunn NM AD - New South Wales Department of Primary Industries and Regional Development, Tamworth Agricultural Institute, Calala, NSW, Australia. FAU - Drynan, Linda J AU - Drynan LJ AD - New South Wales Department of Primary Industries and Regional Development, Tamworth Agricultural Institute, Calala, NSW, Australia. LA - eng GR - the New South Wales Department of Primary Industries and the Australian Cotton Research and Development Corporation (CRDC)/ PT - Journal Article DEP - 20261029 PL - England TA - J Econ Entomol JT - Journal of economic entomology JID - 2985127R SB - IM OTO - NOTNLM OT - cluster caterpillar OT - cross-resistance OT - multi-resistance OT - resistance management OT - resistance monitoring EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 11:53 PHST- 2026/08/12 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 11:53 [entrez] AID - 9004642 [pii] AID - 10.1093/jee/toag328 [doi] PST - aheadofprint SO - J Econ Entomol. 2026 Oct 29:toag328. doi: 10.1093/jee/toag328. PMID- 42955070 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2561-326X (Electronic) IS - 2561-326X (Linking) VI - 10 DP - 2026 Oct 29 TI - Implementation Strategies for a Technology-Based Bullying Bystander Intervention (STAC-T): Mixed Methods Study. PG - e92450 LID - 10.2196/92450 [doi] AB - BACKGROUND: Nearly 1 in 5 students aged 12-18 years report being bullied in the United States. Both targets and students who witness bullying experience negative mental health outcomes. School-based programs are effective in decreasing negative outcomes associated with bullying; however, significant implementation barriers associated with in-person programs limit program adoption, fidelity, and sustainability. STAC-T (Stealing the Show; Turning it Over; Accompanying Others; Coaching Compassion; Technology Version), an interactive bullying bystander app, was developed to reduce implementation barriers. However, little is known about what will be required to successfully translate STAC-T into routine practice in schools. As a first step, we examined school personnel perspectives on school-specific implementation strategies that would be both highly important and feasible ("go-zone" strategies) for delivering STAC-T. Identifying specific implementation strategies can further support implementation and program outcomes. Although researchers have developed a taxonomy of 33 school-specific "go-zone" implementation strategies, there is no research examining these strategies specifically for STAC-T. OBJECTIVE: The aim of this study was to identify implementation strategies for STAC-T using "go-zone" methodology. "Go-zone" methodology categorizes implementation strategies into four zones: (1) zone 1: high importance, high feasibility; (2) zone 2: low importance, high feasibility; (3) zone 3: low importance, low feasibility; and (4) zone: 4 high importance, low feasibility. Objectives included (1) identifying "go-zone" strategies for STAC-T and (2) identifying zone 4 strategies for STAC-T and understanding how to increase the feasibility of these strategies. METHODS: A self-selected convenience sample of school counselors and school social workers (N=195) recruited from national professional organizations completed an online survey. A subsample (n=14) completed qualitative interviews. "Go-zone" methodology was used to identify zone 1 ("go-zone") and zone 4 strategies. Thematic analysis was used to extract themes related to increasing the feasibility of zone 4 strategies. RESULTS: Fifteen implementation strategies were classified as "go-zone" strategies. The three most important strategies were (1) make implementation easier by removing burdensome documentation tasks, (2) develop a detailed implementation plan or blueprint, and (3) obtain and use student and family feedback. The most feasible strategy was distribute educational materials. Four strategies were classified in zone 4: (1) develop instruments to monitor and evaluate core components of the innovative or new practice, (2) conduct educational meetings, (3) build partnerships to support implementation, and (4) provide ongoing consultation. Qualitative data revealed that feasibility of zone 4 strategies can be increased by keeping evaluative assessments short and simple, using various modes of communication for educational meetings, building partnerships with support from school administration, and enhancing consultation through hybrid or virtual meetings and appointing a leader. CONCLUSIONS: Findings represent a first step in identifying implementation strategies that can be used to inform STAC-T implementation. Directions for future research include testing the efficacy of these strategies on improving both implementation and program outcomes. CI - (c)Diana M Doumas, Aida Midgett, Nathanial J Williams, Claudia Peralta. Originally published in JMIR Formative Research (https://formative.jmir.org), 29.10.2026. FAU - Doumas, Diana M AU - Doumas DM AUID- ORCID: 0000-0002-0714-4723 AD - Counselor Education, Boise State University, Boise, ID, United States. FAU - Midgett, Aida AU - Midgett A AUID- ORCID: 0000-0002-0090-0418 AD - Counselor Education, Boise State University, Boise, ID, United States. FAU - Williams, Nathanial J AU - Williams NJ AUID- ORCID: 0000-0002-3948-7480 AD - Counselor Education, Boise State University, Boise, ID, United States. FAU - Peralta, Claudia AU - Peralta C AUID- ORCID: 0000-0002-1826-7972 AD - Counselor Education, Boise State University, Boise, ID, United States. LA - eng PT - Journal Article DEP - 20261029 PL - Canada TA - JMIR Form Res JT - JMIR formative research JID - 101726394 SB - IM MH - *Bullying/prevention & control/psychology MH - Humans MH - Adolescent MH - Child MH - Female MH - Male MH - United States MH - Students/psychology/statistics & numerical data MH - Qualitative Research MH - *Mobile Applications/standards MH - Schools/organization & administration OTO - NOTNLM OT - Accompanying Others OT - Coaching Compassion OT - STAC-T OT - Stealing the Show OT - Technology Version OT - Turning it Over OT - bullying bystander intervention OT - implementation strategies OT - technology-based intervention OT - "go-zone" EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 11:53 PHST- 2026/01/29 00:00 [received] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/09/24 00:00 [revised] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 11:53 [entrez] AID - v10i1e92450 [pii] AID - 10.2196/92450 [doi] PST - epublish SO - JMIR Form Res. 2026 Oct 29;10:e92450. doi: 10.2196/92450. PMID- 42955062 OWN - NLM STAT- Publisher LR - 20261029 IS - 1827-1898 (Electronic) IS - 0031-0808 (Linking) DP - 2026 Oct 29 TI - Fibromyalgia and dysautonomia: towards a neurophysiological framework for personalized management. LID - 10.23736/S0031-0808.26.05437-6 [doi] AB - Fibromyalgia (FM) is a persistent, heterogeneous disorder characterized by widespread pain and multiple non-pain symptoms, including fatigue, cognitive dysfunction, gastrointestinal disturbance, dizziness and balance impairment. Increasing evidence suggests that autonomic dysfunction may contribute to this multisystem presentation, with abnormalities in heart rate variability (HRV), orthostatic cardiovascular regulation and postural orthostatic tachycardia syndrome (POTS) reported in patients with FM. This narrative review examines the evidence for dysautonomia in FM, its relationship with peripheral and central mechanisms, approaches to clinical assessment, and current and emerging strategies for mechanism informed management. A literature search was undertaken using PubMed/MEDLINE, Scopus and PEDro, supplemented by citation searching and relevant grey literature. The search incorporated literature addressing FM, dysautonomia and vestibular rehabilitation, with additional recent literature included to reflect emerging evidence. Evidence supports autonomic dysfunction in a substantial proportion of patients with FM, including reduced HRV, altered sympathovagal regulation, orthostatic intolerance and POTS. Peripheral small fiber pathology and alterations within central autonomic, sensory-processing and stress-response networks may contribute to dysautonomia, supporting interactions between peripheral, central and autonomic mechanisms. Autonomic symptom screening, orthostatic vital signs, HRV and, where indicated, formal autonomic and small fiber investigations may assist in identifying clinically relevant phenotypes. Current management includes lifestyle modification, exercise and rehabilitation, with pharmacological treatment where appropriate. Emerging approaches include HRV biofeedback, non-invasive vagal modulation, vestibular rehabilitation and rehabilitation tailored to the predominant physiological phenotype. Dysautonomia appears to represent a clinically meaningful component of FM in a subgroup of patients rather than a universal feature of the disorder. Recognition of autonomic, peripheral and central contributions may facilitate more targeted clinical assessment and mechanism informed management. Improved phenotyping and further investigation of therapies targeting specific autonomic abnormalities may support more personalized approaches to FM care. FAU - Quodling, Nicole AU - Quodling N AD - The Carrick Institute, FL, USA - nicquod@gmail.com. AD - Coelevate Chiropractic, Adelaide, Australia - nicquod@gmail.com. FAU - Hoffman, Norman AU - Hoffman N AD - The Carrick Institute, FL, USA. FAU - Carrick, Frederick R AU - Carrick FR AD - The Carrick Institute, FL, USA. AD - Burnett School of Biomedical Science, University of Central Florida, Orlando, FL, USA. AD - MGH Institute for Health Professions, Boston, MA, USA. AD - Centre for Mental Health Research in Association with the University of Cambridge, Cambridge, UK. FAU - Jemni, Monem AU - Jemni M AD - The Carrick Institute, FL, USA. AD - College of Medicine, University of Central Florida, Orlando, FL, USA. AD - Faculty of Physical Education, Ningbo University, Ningbo, China. LA - eng PT - Journal Article DEP - 20261029 PL - Italy TA - Panminerva Med JT - Panminerva medica JID - 0421110 SB - IM EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 11:50 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 11:50 [entrez] AID - S0031-0808.26.05437-6 [pii] AID - 10.23736/S0031-0808.26.05437-6 [doi] PST - aheadofprint SO - Panminerva Med. 2026 Oct 29. doi: 10.23736/S0031-0808.26.05437-6. PMID- 42955045 OWN - NLM STAT- Publisher LR - 20261029 IS - 2666-0849 (Electronic) IS - 2666-0849 (Linking) DP - 2026 Sep 2 TI - Apixaban in a Dialysis Patient With Valvular Heart Disease and Calciphylaxis: Rethinking Conventional Anticoagulation. PG - 110196 LID - S2666-0849(26)03357-7 [pii] LID - 10.1016/j.jaccas.2026.110196 [doi] AB - BACKGROUND: Vitamin K antagonists (VKAs) remain the standard anticoagulation for mechanical heart valves but may promote vascular calcification and calciphylaxis. CASE SUMMARY: An 88-year-old woman with a longstanding mechanical aortic valve and advanced chronic kidney disease developed biopsy-confirmed calciphylaxis during VKA therapy. Following initiation of hemodialysis, anticoagulation was switched to low-dose apixaban (2.5 mg twice a day) with plasma level monitoring. Over 2.5 years of follow-up, no bleeding, thromboembolic events, or valve-related complications occurred, and renal recovery allowed dialysis withdrawal. WHY BEYOND THE GUIDELINES?: Direct oral anticoagulants are contraindicated in patients with mechanical heart valves and not recommended in dialysis. Apixaban was introduced because of VKA-associated calciphylaxis and limited therapeutic alternatives. DISCUSSION: Apixaban may be used safely in patients with long-standing mechanical aortic valves because of its predictable pharmacokinetics. TAKE-HOME MESSAGE: Apixaban may represent a pragmatic option in selected patients with long-standing mechanical aortic valves when guideline-recommended VKA therapy cannot be maintained. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Hueso, Miguel AU - Hueso M AD - Department of Nephrology, Hospital Universitari Bellvitge, Institut d'Investigacio Biomedica de Bellvitge-IDIBELL, L'Hospitalet de Llobregat, Spain. Electronic address: mhueso@idibell.cat. FAU - Valverde-Farre, Aida AU - Valverde-Farre A AD - Department of Cardiology, Consorci Sanitari Alt Penedes Garraf, Barcelona, Spain. LA - eng PT - Case Reports PT - Journal Article DEP - 20260902 PL - Netherlands TA - JACC Case Rep JT - JACC. Case reports JID - 101757292 OTO - NOTNLM OT - anticoagulants OT - apixaban OT - calciphylaxis OT - chronic kidney disease OT - heart valve diseases OT - prosthetic heart valves COIS- Funding Support and Author Disclosures The authors have reported that they have no relationships relevant to the contents of this paper to disclose. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 11:36 PHST- 2026/05/01 00:00 [received] PHST- 2026/07/22 00:00 [revised] PHST- 2026/08/05 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 11:36 [entrez] AID - S2666-0849(26)03357-7 [pii] AID - 10.1016/j.jaccas.2026.110196 [doi] PST - aheadofprint SO - JACC Case Rep. 2026 Sep 2:110196. doi: 10.1016/j.jaccas.2026.110196. PMID- 42955038 OWN - NLM STAT- Publisher LR - 20261029 IS - 1558-349X (Electronic) IS - 1546-1440 (Linking) DP - 2026 Oct 29 TI - ACR Appropriateness Criteria(R) Pretreatment Evaluation and Follow-Up of Endometrial Cancer: 2026 Update. LID - S1546-1440(26)00484-9 [pii] LID - 10.1016/j.jacr.2026.09.018 [doi] AB - Endometrial cancer is the most common gynecologic malignancy in the United States, and accurate pretreatment evaluation is essential for risk stratification, treatment planning, and detection of recurrent disease. This evidence-based review summarizes imaging recommendations for patients with established endometrial cancer undergoing pretreatment assessment, surveillance, or evaluation for suspected recurrence. MRI pelvis without and with intravenous contrast is usually appropriate for assessment of local tumor extension across histologic subtypes and grades. For aggressive histologic tumor types, or when metastatic disease is suspected, CT chest, abdomen, and pelvis with intravenous contrast and FDG-PET/CT are usually appropriate for evaluation of nodal and distant metastases. Routine imaging surveillance is usually not appropriate for asymptomatic patients with treated low- or intermediate-risk disease, whereas CT may be appropriate in selected asymptomatic high-risk patients. MRI pelvis, CT chest, abdomen, and pelvis, and FDG-PET/CT are usually appropriate for suspected recurrence. The American College of Radiology Appropriateness Criteria are evidence-based guidelines for specific clinical conditions that are reviewed annually by a multidisciplinary expert panel. The guideline development and revision process support the systematic analysis of the medical literature from peer reviewed journals. Established methodology principles such as Grading of Recommendations Assessment, Development, and Evaluation or GRADE are adapted to evaluate the evidence. The RAND/UCLA Appropriateness Method User Manual provides the methodology to determine the appropriateness of imaging and treatment procedures for specific clinical scenarios. In those instances where peer reviewed literature is lacking or equivocal, experts may be the primary evidentiary source available to formulate a recommendation. CI - Copyright (c) 2026 American College of Radiology. Published by Elsevier Inc. All rights reserved. CN - Expert Panel on GYN and OB Imaging FAU - Sertic, Madeleine AU - Sertic M AD - Joint Department of Medical Imaging, University Health Network, University of Toronto, Toronto, Ontario, Canada. Electronic address: madeleine.sertic@uhn.ca. FAU - Venkatesan, Aradhana M AU - Venkatesan AM AD - Panel Chair, The University of Texas MD Anderson Cancer Center, Houston, Texas. FAU - Suarez-Weiss, Krista AU - Suarez-Weiss K AD - Panel Vice-Chair, Brown University Health, Providence, Rhode Island. FAU - Gupta, Akshya AU - Gupta A AD - University of Rochester, Rochester, New York. FAU - Olinger, Kristen AU - Olinger K AD - University of North Carolina at Chapel Hill, Chapel Hill, North Carolina. FAU - Recht, Hannah AU - Recht H AD - Northwestern University Feinberg School of Medicine, Chicago, Illinois. FAU - Small, William Jr AU - Small W Jr AD - Loyola University Chicago, Stritch School of Medicine, Department of Radiation Oncology, Cardinal Bernardin Cancer Center, Maywood, Illinois; Commission on Radiation Oncology. FAU - Bodurtha Smith, Anna Jo AU - Bodurtha Smith AJ AD - University of Pennsylvania Health System, Philadelphia, Pennsylvania; Society of Gynecologic Oncology. FAU - Stein, Erica B AU - Stein EB AD - University of Michigan Medical Center, Ann Arbor, Michigan. FAU - Feldman, Myra K AU - Feldman MK AD - Specialty Chair, Cleveland Clinic, Cleveland, Ohio. LA - eng PT - Journal Article PT - Practice Guideline DEP - 20261029 PL - United States TA - J Am Coll Radiol JT - Journal of the American College of Radiology : JACR JID - 101190326 SB - IM OTO - NOTNLM OT - AUC OT - Appropriate Use Criteria OT - Appropriateness Criteria OT - Endometrial cancer OT - Imaging evaluation OT - Recurrence OT - Staging EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 11:36 PHST- 2026/09/04 00:00 [received] PHST- 2026/09/12 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 11:36 [entrez] AID - S1546-1440(26)00484-9 [pii] AID - 10.1016/j.jacr.2026.09.018 [doi] PST - aheadofprint SO - J Am Coll Radiol. 2026 Oct 29:S1546-1440(26)00484-9. doi: 10.1016/j.jacr.2026.09.018. PMID- 42955025 OWN - NLM STAT- Publisher LR - 20261030 IS - 1744-7666 (Electronic) IS - 1465-6566 (Linking) DP - 2026 Oct 29 TI - Filgotinib in the treatment of ulcerative colitis: lessons learned from randomized trial to real-world studies. PG - 1-9 LID - 10.1080/14656566.2026.2750245 [doi] AB - INTRODUCTION: Ulcerative colitis (UC) is a chronic relapsing inflammatory bowel disease (IBD) of the colon whose treatment has evolved with novel targeted therapies. Filgotinib (FILGO) is a small molecule that preferentially inhibits Janus kinase 1 (JAK1). The SELECTION trial established the efficacy of FILGO for induction and maintenance of remission in UC, and subsequent real-world studies have confirmed its effectiveness. However, real-world evidence remains limited by relatively small sample sizes and short follow-up periods. Several studies have also explored the ability of FILGO to improve health-related quality of life (HRQoL). AREAS COVERED: This review examines and summarizes current evidence and recent advances regarding filgotinib as a therapeutic option for UC, including efficacy, safety, and real-world effectiveness. PubMed, Embase, and Scopus were searched from database inception through June 2026, supplemented by screening the reference lists of identified articles. EXPERT OPINION: FILGO is a Janus kinase inhibitor (JAKi) with a favorable efficacy and effectiveness profile, as demonstrated by pivotal trials and real-world (RW) studies. Available evidence also suggests a favorable safety profile within the JAK inhibitor class, although randomized head-to-head comparisons are lacking. Further long-term RW studies are needed to confirm these findings and refine the positioning of FILGO in clinical practice. FAU - Mocci, Giammarco AU - Mocci G AD - Division of Gastroenterology, "Brotzu" Hospital, Cagliari, Italy. FAU - Orru, Giorgia AU - Orru G AD - Division of Gastroenterology, "Brotzu" Hospital, Cagliari, Italy. FAU - Onidi, Francesca Maria AU - Onidi FM AD - Division of Gastroenterology, "Brotzu" Hospital, Cagliari, Italy. FAU - Usai Satta, Paolo AU - Usai Satta P AD - Division of Gastroenterology, "Brotzu" Hospital, Cagliari, Italy. FAU - Tursi, Antonio AU - Tursi A AD - Territorial Gastroenterology Service, Barletta-Andria-Trani (BAT) Local Health Agency, Andria, Italy. AD - Department of Medical and Surgical Sciences, Catholic University, School of Medicine, Rome, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - Expert Opin Pharmacother JT - Expert opinion on pharmacotherapy JID - 100897346 SB - IM OTO - NOTNLM OT - Ulcerative colitis OT - filgotinib OT - inflammatory bowel disease OT - remission OT - safety EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 11:35 PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 11:35 [entrez] AID - 10.1080/14656566.2026.2750245 [doi] PST - aheadofprint SO - Expert Opin Pharmacother. 2026 Oct 29:1-9. doi: 10.1080/14656566.2026.2750245. PMID- 42955015 OWN - NLM STAT- Publisher LR - 20261029 IS - 2168-6173 (Electronic) IS - 2168-6165 (Linking) DP - 2026 Oct 29 TI - Bi-Allelic PRKCZ Loss-of-Function Variants and Adult-Onset Retinitis Pigmentosa. LID - 10.1001/jamaophthalmol.2026.4692 [doi] AB - IMPORTANCE: Inherited retinal diseases are genetically heterogeneous disorders, with retinitis pigmentosa (RP) representing the most common form; although more than 90 genes have been implicated, many RP cases remain molecularly unsolved. OBJECTIVES: To describe an adult-onset RP phenotype associated with loss-of-function variants in PRKCZ, profile the clinical features of this disorder, and investigate the underlying disease mechanism. DESIGN, SETTING, AND PARTICIPANTS: In this case series study, genomic analyses were performed in individuals with unsolved childhood- and adult-onset retinal disease recruited through international research and diagnostic programs. Detailed clinical characterization was conducted in affected individuals. In vitro analyses were carried out to validate the genomic findings using primary and transduced cell-based experimental approaches. Genomic analyses were performed from October 2023 to December 2025; functional studies were carried out from January 2024 to May 2026. EXPOSURE: Bi-allelic likely pathogenic variants in a novel RP disease gene candidate, PRKCZ. MAIN OUTCOMES AND MEASURES: The primary outcome was genetic, clinical, and functional effects of bi-allelic PRKCZ inactivating variants associated with adult-onset RP. A detailed ophthalmologic assessment was conducted to characterize the clinical phenotype. Functional analyses in patient-derived fibroblasts and transduced cell lines were performed to validate the consequence of disruptive variants on PRKCZ transcript processing and PKCzeta function. RESULTS: Among approximately 4500 individuals with unsolved childhood- and adult-onset retinal disease tested, 6 bi-allelic PRKCZ variants were identified in 13 affected individuals from 10 unrelated families, 5 of which were predicted to lead to loss of function (frameshift, nonsense, or splice-altering variants). Six of 13 affected individuals (46%) were female. Most cases carried a recurrent frameshift variant (c.139_140del; p.Met47Valfs*19). All variants selectively affected the long PRKCZ transcript encoding the N-terminal Phox and Bem1 (PB1) domain. Clinically, PRKCZ-associated RP showed adult onset (18-49 years), progressive night blindness, peripheral visual field constriction, and eventual central vision loss. Four individuals exhibited adult-onset sensorineural hearing loss. Functional studies in patient-derived fibroblasts demonstrated selective loss of the long PRKCZ isoform, impaired ciliogenesis, and altered mitochondrial organization, which were validated in stable PRKCZ knockdown cell lines. Structural modeling and coimmunoprecipitation assays indicated that the missense variant was associated with the disruption of PB1 domain-mediated protein-protein interactions. CONCLUSIONS AND RELEVANCE: In this case series study, bi-allelic inactivating variants in PRKCZ were associated with adult-onset RP with an isoform-specific mechanism involving the long retinal-enriched transcript. These findings expand the genetic spectrum of RP and highlight PRKCZ as a potentially clinically relevant gene implicated in adult-onset retinal degeneration. FAU - Cordeddu, Viviana AU - Cordeddu V AD - Department of Oncology and Molecular Medicine, Istituto Superiore di Sanita, Rome, Italy. FAU - Sangermano, Riccardo AU - Sangermano R AD - Ocular Genomics Institute, Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts. FAU - Mulder, Sterre W M AU - Mulder SWM AD - Department of Human Genetics, Radboud University Medical Center, Nijmegen, the Netherlands. AD - Lifelong VISION consortium, the Netherlands. FAU - Hussain, Hafiz Muhammad Jafar AU - Hussain HMJ AD - Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas. FAU - Flex, Elisabetta AU - Flex E AD - Department of Oncology and Molecular Medicine, Istituto Superiore di Sanita, Rome, Italy. FAU - Mignini, Luca AU - Mignini L AD - Molecular Genetics and Functional Genomics Research Division, Bambino Gesu Children's Hospital, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rome, Italy. FAU - Motta, Marialetizia AU - Motta M AD - Molecular Genetics and Functional Genomics Research Division, Bambino Gesu Children's Hospital, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rome, Italy. FAU - Bruselles, Alessandro AU - Bruselles A AD - Department of Oncology and Molecular Medicine, Istituto Superiore di Sanita, Rome, Italy. FAU - Arasi, Maria Beatrice AU - Arasi MB AD - Department of Oncology and Molecular Medicine, Istituto Superiore di Sanita, Rome, Italy. AD - Molecular Genetics and Functional Genomics Research Division, Bambino Gesu Children's Hospital, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rome, Italy. FAU - Cecchetti, Serena AU - Cecchetti S AD - Confocal Microscopy Unit-Core Facilities, Istituto Superiore di Sanita, Rome, Italy. FAU - Carvetta, Mattia AU - Carvetta M AD - Molecular Genetics and Functional Genomics Research Division, Bambino Gesu Children's Hospital, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rome, Italy. AD - Department of Biochemical Sciences "Alessandro Rossi Fanelli," Sapienza University of Rome, Rome, Italy. FAU - Niceta, Marcello AU - Niceta M AD - Molecular Genetics and Functional Genomics Research Division, Bambino Gesu Children's Hospital, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rome, Italy. FAU - Lauri, Antonella AU - Lauri A AD - Molecular Genetics and Functional Genomics Research Division, Bambino Gesu Children's Hospital, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rome, Italy. FAU - Leone, Alessandro AU - Leone A AD - Department of Oncology and Molecular Medicine, Istituto Superiore di Sanita, Rome, Italy. AD - Department of Humanities, Law and Economics, Universita Telematica Leonardo da Vinci (UNIDAV), Torrevecchia Teatina, Chieti, Italy. FAU - Paradisi, Graziamaria AU - Paradisi G AD - Molecular Genetics and Functional Genomics Research Division, Bambino Gesu Children's Hospital, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rome, Italy. FAU - Mehta, Puja A AU - Mehta PA AD - Ocular Genomics Institute, Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts. AD - Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts. AD - Broad Institute of Harvard and MIT, Cambridge, Massachusetts. FAU - Yumei, Li AU - Yumei L AD - Department of Ophthalmology and Visual Sciences, Robert M. Brunson Center for Translational Vision Research, Irvine School of Medicine, University of California, Irvine. FAU - Wang, Meng AU - Wang M AD - Department of Ophthalmology and Visual Sciences, Robert M. Brunson Center for Translational Vision Research, Irvine School of Medicine, University of California, Irvine. FAU - Zein, Wadih M AU - Zein WM AD - Ophthalmic Genetics and Visual Function Branch, National Eye Institute, National Institutes of Health, Bethesda, Maryland. FAU - Shliaga, Stefanida AU - Shliaga S AD - Department of Human Genetics, Radboud University Medical Center, Nijmegen, the Netherlands. FAU - Wigman, Lonneke Haer AU - Wigman LH AD - Department of Human Genetics, Radboud University Medical Center, Nijmegen, the Netherlands. FAU - van Leeuwen, Yoeri AU - van Leeuwen Y AD - Department of Ophthalmology, Donders Institute for Brain, Cognition and Behaviour, Radboud University Medical Center, Nijmegen, the Netherlands. FAU - Klaver, Caroline C W AU - Klaver CCW AD - Lifelong VISION consortium, the Netherlands. AD - Department of Ophthalmology, Donders Institute for Brain, Cognition and Behaviour, Radboud University Medical Center, Nijmegen, the Netherlands. AD - Department of Ophthalmology, Erasmus Medical Center, Rotterdam, the Netherlands. AD - Department of Epidemiology, Erasmus Medical Center, Rotterdam, the Netherlands. AD - Institute of Molecular and Clinical Ophthalmology, Basel, Switzerland. FAU - Pennings, Ronald AU - Pennings R AD - Department of Otorhinolaryngology, Radboud University Medical Center, Nijmegen, the Netherlands. FAU - van den Born, L Ingeborgh AU - van den Born LI AD - The Rotterdam Eye Hospital, BH Rotterdam, the Netherlands. FAU - Segre, Ayellet V AU - Segre AV AD - Ocular Genomics Institute, Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts. AD - Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts. AD - Broad Institute of Harvard and MIT, Cambridge, Massachusetts. FAU - Pierce, Eric A AU - Pierce EA AD - Ocular Genomics Institute, Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts. FAU - Collin, Rob W J AU - Collin RWJ AD - Department of Human Genetics, Radboud University Medical Center, Nijmegen, the Netherlands. AD - Lifelong VISION consortium, the Netherlands. FAU - Dell'Aquila, Carmen AU - Dell'Aquila C AD - Clinical and Research Center of Neurophthalmology and Genetic and Rare Diseases of the Eye, IRCCS-Fondazione Bietti, Rome, Italy. FAU - Barbano, Lucilla AU - Barbano L AD - Clinical and Research Center of Neurophthalmology and Genetic and Rare Diseases of the Eye, IRCCS-Fondazione Bietti, Rome, Italy. FAU - Guan, Bin AU - Guan B AD - Ophthalmic Genetics and Visual Function Branch, National Eye Institute, National Institutes of Health, Bethesda, Maryland. FAU - Place, Emily M AU - Place EM AD - Ocular Genomics Institute, Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts. FAU - Lin, Siying AU - Lin S AD - Division of Evolution, Infection and Genomics, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester, United Kingdom. AD - Manchester Centre for Genomic Medicine, Saint Mary's Hospital and Department of Ophthalmology, Manchester Royal Eye Hospital, Manchester University NHS Foundation Trust, Manchester, United Kingdom. AD - National Institute of Health Research Biomedical Research Centre, Moorfields Eye Hospital NHS Foundation Trust, London, United Kingdom. AD - Institute of Ophthalmology, University College London, London, United Kingdom. FAU - Webster, Andrew R AU - Webster AR AD - National Institute of Health Research Biomedical Research Centre, Moorfields Eye Hospital NHS Foundation Trust, London, United Kingdom. AD - Institute of Ophthalmology, University College London, London, United Kingdom. FAU - Moosajee, Mariya AU - Moosajee M AD - Institute of Ophthalmology, University College London, London, United Kingdom. AD - The Francis Crick Institute, London, United Kingdom. FAU - Chen, Rui AU - Chen R AD - Department of Ophthalmology and Visual Sciences, Robert M. Brunson Center for Translational Vision Research, Irvine School of Medicine, University of California, Irvine. FAU - Arno, Gavin AU - Arno G AD - National Institute of Health Research Biomedical Research Centre, Moorfields Eye Hospital NHS Foundation Trust, London, United Kingdom. AD - Institute of Ophthalmology, University College London, London, United Kingdom. AD - Division of Research, Greenwood Genetic Center, Greenwood, South Carolina. FAU - Roosing, Susanne AU - Roosing S AD - Department of Human Genetics, Radboud University Medical Center, Nijmegen, the Netherlands. AD - Lifelong VISION consortium, the Netherlands. FAU - Ziccardi, Lucia AU - Ziccardi L AD - Clinical and Research Center of Neurophthalmology and Genetic and Rare Diseases of the Eye, IRCCS-Fondazione Bietti, Rome, Italy. AD - Department of Medicine and Health Sciences "V. Tiberio," University of Molise, Campobasso, Italy. FAU - Bujakowska, Kinga M AU - Bujakowska KM AD - Ocular Genomics Institute, Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts. FAU - Tartaglia, Marco AU - Tartaglia M AD - Molecular Genetics and Functional Genomics Research Division, Bambino Gesu Children's Hospital, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rome, Italy. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - JAMA Ophthalmol JT - JAMA ophthalmology JID - 101589539 SB - IM EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 11:35 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 11:35 [entrez] AID - 2855507 [pii] AID - 10.1001/jamaophthalmol.2026.4692 [doi] PST - aheadofprint SO - JAMA Ophthalmol. 2026 Oct 29. doi: 10.1001/jamaophthalmol.2026.4692. PMID- 42955008 OWN - NLM STAT- Publisher LR - 20261029 IS - 2374-2445 (Electronic) IS - 2374-2437 (Linking) DP - 2026 Oct 29 TI - FGFR Alterations in Bladder Cancer-From Molecular Pathogenesis to Precision Oncology. LID - 10.1001/jamaoncol.2026.4196 [doi] AB - IMPORTANCE: Alterations in the fibroblast growth factor receptor (FGFR) pathway, particularly FGFR3, are among the most common genomic events in urothelial carcinoma (UC) and represent a cornerstone of precision oncology. Although FGFR3 alterations predominate, selected FGFR2 fusions also identify patients who may benefit from targeted therapy. This review summarizes the biology, molecular diagnostics, and evolving clinical applications of FGFR alterations in UC. OBSERVATIONS: FGFR2/3 alterations are clinically actionable biomarkers across non-muscle-invasive, muscle-invasive, metastatic, and upper-tract UC. Comprehensive genomic profiling, tissue sequencing, plasma circulating tumor DNA, urine-based molecular assays, and molecular residual disease assessment are expanding their roles in diagnosis, treatment selection, surveillance, and disease monitoring. Recent clinical studies support combination strategies, next-generation FGFR inhibitors, and earlier use of FGFR-directed therapy, while emerging technologies, including artificial intelligence-assisted pathology and integrated multi-omic biomarkers, may further improve patient selection. CONCLUSIONS AND RELEVANCE: FGFR alterations have become integral to the molecular classification and precision management of UC. Continued advances in molecular diagnostics, liquid biopsy, biomarker-guided therapy, and next-generation FGFR inhibitors are expected to further personalize care. This review provides a practical framework for integrating FGFR biomarkers into contemporary management and highlights emerging developments likely to influence future clinical practice. FAU - Collins, Katrina AU - Collins K AD - Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston. FAU - Cheng, Liang AU - Cheng L AD - Department of Pathology and Laboratory Medicine, Brown University Warren Alpert Medical School, Providence, Rhode Island. AD - Division of Urology, Department of Surgery, Brown University Warren Alpert Medical School, Providence, Rhode Island. AD - Legorreta Cancer Center at Brown University, Brown University Health, Providence, Rhode Island. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - JAMA Oncol JT - JAMA oncology JID - 101652861 SB - IM EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 11:34 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 11:34 [entrez] AID - 2856001 [pii] AID - 10.1001/jamaoncol.2026.4196 [doi] PST - aheadofprint SO - JAMA Oncol. 2026 Oct 29. doi: 10.1001/jamaoncol.2026.4196. PMID- 42954991 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2574-3805 (Electronic) IS - 2574-3805 (Linking) VI - 9 IP - 10 DP - 2026 Oct 1 TI - Data Extraction Errors and Diagnostic Conclusions of Meta-Analyses: A Systematic Review. PG - e2640850 LID - 10.1001/jamanetworkopen.2026.40850 [doi] AB - IMPORTANCE: Systematic reviews (SRs) are often characterized as being inherently reproducible; however, errors arising from the evidence synthesis process can compromise this assumption and lead to reproducibility failure. OBJECTIVE: To investigate the data extraction errors in diagnostic meta-analyses and the extent to which such errors affect the results of meta-analyses. EVIDENCE REVIEW: We included SRs with diagnostic meta-analyses from the Cochrane Library filtered by diagnostic up to May 2023. Data used in the original meta-analyses were extracted from the included SRs and independently reproduced from primary studies. Data extraction errors were identified by comparing datasets and classified by potential mechanism. To assess the consequences, we reanalyzed the meta-analyses using error-corrected data with the same effect estimators and methods. The primary outcomes were the proportions of data extraction errors at the trial, meta-analysis, and SR levels. The secondary outcome was the consequences for diagnostic performance measures (eg, sensitivity, specificity, diagnostic odds ratio [DOR], and area under the curve [AUC]). Analysis was conducted between March and June 2026. FINDINGS: A total of 46 SRs, 879 diagnostic comparisons, and 6173 primary studies were included. Data extraction could not be reproduced in 338 studies (5.5%) and affected 137 diagnostic meta-analyses (33.1%) and 34 SRs (73.9%). Numerical error (ie, incorrect values) and inferential error (ie, incorrect derivation from statistics) were the most prevalent error types, accounting for 166 of 412 (40.3%) and 140 of 412 (34.0%) errors, respectively. Mismatching error (ie, mismatching data to groups; 69 of 412 errors [16.7%]) and conceptual error (ie, misaligned reference details; 37 of 412 [9.0%]) were also identified. Among 137 reproduced meta-analyses, large changes were observed in 0.7% meta-analyses for sensitivity (absolute differences, >0.20), 3.6% for DOR (fold-changes, >5.0), and 2.9% for AUC (absolute differences, >0.10). Regression analysis of error location showed that combined false-positive and true-negative errors were associated with higher likelihood of moderate or large DOR changes (OR, 1.11; 95% CI, 1.01-1.22). CONCLUSIONS AND RELEVANCE: In this SR of diagnostic meta-analyses in Cochrane reviews, data extraction errors were common and differentially affected performance measures, highlighting the need to implement rigorous data extraction processes and to consider careful selection of performance measures. FAU - Huang, Zixing AU - Huang Z AD - Department of Radiology, West China Hospital of Sichuan University, Chengdu, China. FAU - Tian, Yuan AU - Tian Y AD - Department of Orthopedics, The First Hospital of China Medical University, China Medical University, Shenyang, China. FAU - Gong, Ping AU - Gong P AD - School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, China. FAU - Zhang, Tong AU - Zhang T AD - School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, China. FAU - Tang, Hehan AU - Tang H AD - Department of Radiology, West China Hospital of Sichuan University, Chengdu, China. LA - eng PT - Journal Article PT - Systematic Review DEP - 20261001 PL - United States TA - JAMA Netw Open JT - JAMA network open JID - 101729235 SB - IM MH - *Meta-Analysis as Topic MH - Humans MH - Reproducibility of Results MH - *Systematic Reviews as Topic MH - Sensitivity and Specificity EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 11:34 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 11:34 [entrez] AID - 2855945 [pii] AID - 10.1001/jamanetworkopen.2026.40850 [doi] PST - epublish SO - JAMA Netw Open. 2026 Oct 1;9(10):e2640850. doi: 10.1001/jamanetworkopen.2026.40850. PMID- 42954986 OWN - NLM STAT- Publisher LR - 20261029 IS - 2168-619X (Electronic) IS - 2168-6181 (Linking) DP - 2026 Oct 29 TI - Nasal Obstruction and Treatment With Oxymetazoline and Corticosteroids: A Randomized Clinical Trial. LID - 10.1001/jamaoto.2026.3297 [doi] AB - IMPORTANCE: Prolonged use of oxymetazoline, a highly effective nasal decongestant, is associated with rebound obstruction, but previous literature has shown potential for intranasal steroids to prevent tachyphylaxis and rebound, thereby potentially allowing for long-term combined oxymetazoline/intranasal steroid use in patients with chronic nasal obstruction. OBJECTIVE: To determine whether long-term combined oxymetazoline/budesonide nasal spray could alleviate the nasal obstruction associated with chronic rhinitis without inducing symptoms or histopathologic evidence of rhinitis medicamentosa. DESIGN, SETTING, AND PARTICIPANTS: This study was a single-center, double-blind, randomized clinical trial conducted at Washington University in St Louis/Barnes Jewish Hospital from October 2024 to July 2025. Key selection criteria included adults with chronic nasal obstruction, prior failure of topical steroids, and no chronic sinusitis. INTERVENTION: Participants used a provided nasal spray twice daily containing either oxymetazoline/budesonide or budesonide alone for 4 weeks of active treatment followed by a 3-week wean period. Ten participants underwent pretreatment and posttreatment inferior turbinate biopsies for histopathological analysis of change. MAIN OUTCOMES AND MEASURES: The primary outcome measure is the rate of responders, defined as at least moderate improvement on the Clinical Global Impressions-Improvement scale, assessed after 4 weeks of treatment. Measures of rebound obstruction or difficulty weaning were assessed by score changes on the Clinical Global Impression-Severity scale. RESULTS: From 87 individuals screened, 61 participants signed informed consent, and 60 participants were randomized and completed baseline assessments. Of the randomized participants, 31 participants received oxymetazoline/budesonide spray (mean [SD] age at enrollment, 46.0 [18.7] years; 18 [58.1%] female; 13 [41.9%] male), and 29 participants received budesonide alone (mean [SD] age, 51.2 [17.1] years; 17 [58.6%] female; 12 [41.4%] male). There were 24 of 29 (83%) responders to treatment in the oxymetazoline/budesonide arm, compared with 10 of 28 (36%) in the budesonide-only arm, a difference of 47 percentage points (95% CI, 25-70 percentage points). None of the participants in the oxymetazoline arm developed worsening obstruction concerning for rebound obstruction during active treatment. All successfully weaned off oxymetazoline without issue. CONCLUSIONS AND RELEVANCE: In this randomized clinical trial, there was a large and clinically meaningful greater proportion of responders in the oxymetazoline arm, without signs or symptoms of rhinitis medicamentosa during the study. All participants successfully weaned off oxymetazoline. For patients with chronic nasal obstruction failing topical steroids, chronic therapy with combination oxymetazoline/budesonide may be considered, though risks of rebound congestion with longer-term use cannot be excluded. TRIAL REGISTRATION: ClinicalTrials.gov Identifier: NCT06830109. FAU - Cruz, Lucas AU - Cruz L AD - Washington University School of Medicine, St Louis, Missouri. FAU - Rammell, Jake AU - Rammell J AD - Washington University School of Medicine, St Louis, Missouri. FAU - Kallogjeri, Dorina AU - Kallogjeri D AD - Department of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St Louis, Missouri. AD - Statistics Editor, JAMA Otolaryngology-Head & Neck Surgery. FAU - Piccirillo, Jay F AU - Piccirillo JF AD - Department of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St Louis, Missouri. AD - Editor, JAMA Otolaryngology-Head & Neck Surgery. FAU - Agboola, Tomilola AU - Agboola T AD - Department of Pathology & Immunology, Washington University School of Medicine, St Louis, Missouri. FAU - Schwetye, Katherine E AU - Schwetye KE AD - Department of Pathology & Immunology, Washington University School of Medicine, St Louis, Missouri. FAU - Roland, Lauren T AU - Roland LT AD - Department of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St Louis, Missouri. FAU - Schneider, John AU - Schneider J AD - Department of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St Louis, Missouri. FAU - Farrell, Nyssa Fox AU - Farrell NF AD - Department of Otolaryngology-Head and Neck Surgery, Washington University School of Medicine, St Louis, Missouri. LA - eng SI - ClinicalTrials.gov/NCT06830109 PT - Journal Article DEP - 20261029 PL - United States TA - JAMA Otolaryngol Head Neck Surg JT - JAMA otolaryngology-- head & neck surgery JID - 101589542 SB - IM EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 11:34 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 11:34 [entrez] AID - 2855938 [pii] AID - 10.1001/jamaoto.2026.3297 [doi] PST - aheadofprint SO - JAMA Otolaryngol Head Neck Surg. 2026 Oct 29. doi: 10.1001/jamaoto.2026.3297. PMID- 42954942 OWN - NLM STAT- Publisher LR - 20261029 IS - 1364-6915 (Electronic) IS - 1360-7863 (Linking) DP - 2026 Oct 29 TI - Beyond listening: music engagement and mood in later life. PG - 1-10 LID - 10.1080/13607863.2026.2749610 [doi] AB - Objectives: Music engagement has been associated with cognitive, emotional, social, and physical benefits in older adulthood. Less is known about how different forms of music engagement relate to overall mood. This study examined whether distinct approaches to music engagement predicted mood disturbance among older adults. Methods: Forty-three adults were recruited and after treatment for missing data, forty adults aged 60 years and older completed the Music USE Questionnaire and the Profile of Mood States-Short Form and were included in the analyses. Hierarchical linear regression analyses examined whether cognitive and emotional regulation, social connection, engaged production, physical exercise, and dance predicted total mood disturbance. Results: A significant negative correlation emerged between engaged production and mood disturbance. Engaged production was negatively correlated with mood disturbance and significantly predicted mood disturbance. Other forms of music engagement (i.e. social connectedness and cognitive and emotion regulation) were not significantly associated with mood disturbance. Conclusion: These preliminary findings identify active music-making as a potential correlate of less mood disturbance in later life. These preliminary findings identify engaged production as a potentially meaningful correlate of less mood disturbance in later life, while highlighting the need for larger, longitudinal studies to clarify the nature and direction of this association. FAU - Peeples, L Blake AU - Peeples LB AD - Department of Psychological and Brain Sciences, The University of Louisville, Louisville, Kentucky, USA. FAU - Rooseboom, Tessa AU - Rooseboom T AD - Department of Psychology, University of Prince Edward Island, Faculty of Arts, Charlottetown, Prince Edward Island, Canada. FAU - Strong, Jessica V AU - Strong JV AD - Department of Psychology, University of Prince Edward Island, Faculty of Arts, Charlottetown, Prince Edward Island, Canada. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Aging Ment Health JT - Aging & mental health JID - 9705773 SB - IM OTO - NOTNLM OT - Older adults OT - mood OT - music engagement OT - music-making OT - socioemotional selectivity theory EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 11:23 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 11:23 [entrez] AID - 10.1080/13607863.2026.2749610 [doi] PST - aheadofprint SO - Aging Ment Health. 2026 Oct 29:1-10. doi: 10.1080/13607863.2026.2749610. PMID- 42954941 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1759-9679 (Electronic) IS - 1759-9660 (Linking) VI - 18 IP - 41 DP - 2026 Oct 29 TI - Chiral L-histidine-ZIF-8/MWCNTs-based molecularly imprinted electrochemical sensor for L-tryptophan. PG - 9773-9783 LID - 10.1039/d6ay01796g [doi] AB - A molecularly imprinted electrochemical sensor based on chiral L-histidine (L-His)-ZIF-8/multi-walled carbon nanotubes (MWCNTs) was developed for sensitive and selective detection of L-tryptophan (L-Trp). The L-His-ZIF-8/MWCNTs composite combines the chiral recognition environment of L-His-ZIF-8 with the favorable electron-transfer properties of MWCNTs, providing an effective electrode interface for target enrichment and electrochemical sensing. Compared with achiral ZIF-8/MWCNTs, the chiral composite exhibited a markedly higher adsorption capacity toward L-Trp, which is beneficial for increasing target accumulation at the sensing interface before molecularly imprinted polymerization. Molecularly imprinted layers were subsequently constructed by electropolymerization of o-phenylenediamine in the presence of beta-cyclodextrin and L-Trp. After removing the template, "imprinted cavities" with specific recognition capabilities were formed, thereby enabling the selective detection of L-Trp. Under optimized conditions, the sensor exhibited a wide linear response from 1 nM to 1 mM with a detection limit of 0.34 nM, together with good selectivity, reproducibility, and stability. The sensor was further applied to L-Trp determination in milk samples with satisfactory recoveries, demonstrating its potential for the analysis of chiral amino acids in complex samples. FAU - Wang, Ziwei AU - Wang Z AD - Department of Chemistry, Capital Normal University, Beijing 100048, China. mazhanfang@cnu.edu.cn. FAU - Liu, Mianfeng AU - Liu M AD - Department of Chemistry, Capital Normal University, Beijing 100048, China. mazhanfang@cnu.edu.cn. FAU - Li, Jiawei AU - Li J AD - Department of Chemistry, Capital Normal University, Beijing 100048, China. mazhanfang@cnu.edu.cn. FAU - Han, Hongliang AU - Han H AD - Department of Chemistry, Capital Normal University, Beijing 100048, China. mazhanfang@cnu.edu.cn. FAU - Ma, Zhanfang AU - Ma Z AUID- ORCID: 0000-0002-2144-3957 AD - Department of Chemistry, Capital Normal University, Beijing 100048, China. mazhanfang@cnu.edu.cn. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Anal Methods JT - Analytical methods : advancing methods and applications JID - 101519733 RN - 4QD397987E (Histidine) RN - 8DUH1N11BX (Tryptophan) RN - 0 (Nanotubes, Carbon) SB - IM MH - *Histidine/chemistry MH - *Molecular Imprinting/methods MH - *Tryptophan/analysis MH - *Nanotubes, Carbon/chemistry MH - *Electrochemical Techniques/methods MH - Limit of Detection MH - Animals MH - Stereoisomerism MH - Reproducibility of Results MH - Milk/chemistry EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 11:23 PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 11:23 [entrez] AID - 10.1039/d6ay01796g [doi] PST - epublish SO - Anal Methods. 2026 Oct 29;18(41):9773-9783. doi: 10.1039/d6ay01796g. PMID- 42954936 OWN - NLM STAT- Publisher LR - 20261029 IS - 1866-0452 (Electronic) IS - 1866-0452 (Linking) VI - 123 IP - 2 DP - 2027 Jan 12 TI - ANA Testing From Background Prevalence to Pretest Probability: A Recommendation for Routine Practice. LID - arztebl.m2026.0189 [pii] LID - 10.3238/arztebl.m2026.0189 [doi] AB - BACKGROUND: Antinuclear antibodies (ANA, now correctly designated as the HEp-2 immunofluorescence test) are a commonly ordered laboratory test. In one regional laboratory that was asked to provide figures, the number of ANA tests carried out per year doubled from 2019 to 2025. This review provides a detailed explanation of the utility of ANA testing and the interpretation of positive findings. METHODS: The positive predictive value (PPV) of a positive ANA test and its clinical interpretation were studied with reference to the background prevalence of ANA and the prevalence of ANA-associated rheumatic diseases, e.g., systemic lupus erythematosus (SLE) and systemic sclerosis. This narrative review is based on publications that were retrieved by a selective search on the topics of ANA prevalence and the diagnostic value of ANA testing and its clinical indications, with special attention to population-based and clinical studies and pertinent consensus recommendations. RESULTS: The estimated overall ANA positivity rate (background prevalence) ranges from 13% to 33% in population-based studies. The PPV strongly depends on the cohort studied, the clinical pretest probability of an ANA-associated disease, and the antibody concentration (titer) required for a positive test. In a rheumatological referral cohort, the PPV of the test was 2.1% for SLE and 9.1% for ANA-associated rheumatic diseases. Large cohort studies have shown that relatively few ANA-positive persons have an ANA-associated rheumatic disease; in a Korean study, for example, the figure was 4.7%. Thus, a positive ANA test alone does not establish the diagnosis. Its clinical relevance depends on the constellation of disease manifestations, antibody titer, fluorescence pattern, and (where applicable) disease-specific autoantibodies. CONCLUSION: ANA should only be measured if there is a well-founded clinical suspicion of an ANA-associated disease, not as a general screening test for patients with nonspecific symptoms such as isolated fatigue, pain of undetermined origin, or pain in a single joint. Positive test findings require clinical contextualization first and foremost, rather than automated follow-up laboratory testing. Serial ANA tests are not suitable for determining the activity level of a rheumatic disease or for monitoring the effects of treatment. The symptom- and test-finding-based approach following the Choosing Wisely recommendations can help minimize the risk of overdiagnosis, needless consultations, and unnecessary follow-up. FAU - Krasselt, Marco AU - Krasselt M LA - eng PT - Journal Article PT - Review DEP - 20270112 PL - Germany TA - Dtsch Arztebl Int JT - Deutsches Arzteblatt international JID - 101475967 SB - IM EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 11:13 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 11:13 [entrez] AID - arztebl.m2026.0189 [pii] AID - 10.3238/arztebl.m2026.0189 [doi] PST - aheadofprint SO - Dtsch Arztebl Int. 2027 Jan 12;123(2):arztebl.m2026.0189. doi: 10.3238/arztebl.m2026.0189. PMID- 42954935 OWN - NLM STAT- Publisher LR - 20261029 IS - 1866-0452 (Electronic) IS - 1866-0452 (Linking) VI - 123 IP - 2 DP - 2027 Jan 12 TI - Clinical Practice Guideline: Palliative Care for Children and Adolescents With Cancer. LID - arztebl.m2026.0188 [pii] LID - 10.3238/arztebl.m2026.0188 [doi] AB - BACKGROUND: Despite recent advances in pediatric oncology, 15% of children and adolescents with cancer in Germany (approx. 350 per year) die of their disease. Even when the unfavorable prognosis is clear early on, patients often do not receive palliative care until shortly before death when the symptom burden is high. This causes unnecessary distress for everyone involved. METHODS: Systematic searches for pertinent guidelines and scientific publications up to 2023/2024 were carried out in accordance with the AWMF specifications for S3-level clinical practice guidelines. Of the 17 821 primary publications screened (databases: PubMed, Embase, Cochrane), 66 were used as sources of evidence for the guideline recommendations. Individual recommendations from three adult guidelines were also adopted after appropriate adjustment for the special situation and needs of children and adolescents. RESULTS: Evidence levels were mostly low or very low. 68 recommendations were developed, including: for care structures, the proactive implementation of specialized palliative care for patients with an unfavorable prognosis; for advance care planning, the need for open, structured communication processes and for support with the creation of advance care documents; for dyspnea (prevalence, 12-80%), the central role and safety of opioids and non-pharmacological measures such as positioning and ventilator therapy; for pain (prevalence, 62-92%), the importance of a comprehensive, multimodal approach, with numerous recommendations concerning medications (selection and application), non-pharmacological measures (such as positioning and distraction), and the importance of communication, patient education, and the structured assessment of pain. CONCLUSION: Controlled trials are needed to provide higher-level evidence on palliative care interventions for children and adolescents with cancer. The close collaboration of pediatric oncologists and specialists in pediatric palliative medicine is a key component of patient- and family-centered care. FAU - Fuhrer, Monika AU - Fuhrer M FAU - Baumler, Petra AU - Baumler P FAU - Classen, Carl Friedrich AU - Classen CF FAU - Heitkamp, Nari AU - Heitkamp N FAU - Voelker, Thomas AU - Voelker T FAU - Irnich, Dominik AU - Irnich D CN - PAED-ONCO-PALL guideline group LA - eng PT - Journal Article PT - Practice Guideline DEP - 20270112 PL - Germany TA - Dtsch Arztebl Int JT - Deutsches Arzteblatt international JID - 101475967 SB - IM EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 11:13 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 11:13 [entrez] AID - arztebl.m2026.0188 [pii] AID - 10.3238/arztebl.m2026.0188 [doi] PST - aheadofprint SO - Dtsch Arztebl Int. 2027 Jan 12;123(2):arztebl.m2026.0188. doi: 10.3238/arztebl.m2026.0188. PMID- 42954929 OWN - NLM STAT- Publisher LR - 20261029 IS - 1539-2864 (Electronic) IS - 0275-004X (Linking) DP - 2026 Oct 29 TI - Association of Retinal Microvascular Density with Hemodynamic and Clinical Severity Markers in Pulmonary Arterial Hypertension. LID - 10.1097/IAE.0000000000005043 [doi] AB - PURPOSE: To evaluate whether quantitative retinal microvascular and structural parameters, measured by optical coherence tomography angiography (OCTA), are associated with disease severity and right ventriculo-arterial coupling in patients with pulmonary arterial hypertension (PAH). METHODS: This cross-sectional study included 45 patients with confirmed PAH, stratified by New York Heart Association (NYHA) functional class (I-III). Patients with coexisting ocular pathologies were strictly excluded. Vessel density (VD) in the superficial (SCP) and deep capillary plexuses (DCP), foveal avascular zone characteristics, and ganglion cell complex (GCC) thickness were analyzed. These parameters were correlated with functional capacity (6-minute walk test [6-mWT]), NT-proBNP levels, and the echocardiographic TAPSE/sPAP ratio. RESULTS: Retinal VD in both SCP and DCP progressively decreased with worsening NYHA functional class (p < 0.05). Retinal VD showed a significant negative correlation with NT-proBNP levels (r = -0.355, p = 0.017) and a positive correlation with 6-mWT distance (r = 0.499, p < 0.001). Crucially, retinal microvascular density demonstrated a robust positive correlation with the TAPSE/sPAP ratio (r = 0.420, p = 0.004), a surrogate for right ventriculo-arterial coupling. GCC thickness exhibited variations across NYHA classes but lacked a consistent linear progression. CONCLUSION: Retinal microvascular attenuation in PAH is strongly associated not only with functional and biochemical severity but also with central hemodynamic impairment represented by the TAPSE/sPAP ratio. These findings suggest that OCTA-derived vessel density may serve as a promising, non-invasive biomarker for monitoring systemic microvascular compromise and right heart function in PAH. FAU - Erdag, Murat AU - Erdag M AUID- ORCID: 0000-0001-8857-994 AD - Department of Ophthalmology, Faculty of Medicine, Firat University, 23119 Elazig, Turkiye. FAU - Caliskan, Tunahan AU - Caliskan T AD - Department of Ophthalmology, Faculty of Medicine, Firat University, 23119 Elazig, Turkiye. FAU - Yildirim, Hakan AU - Yildirim H AD - Department of Ophthalmology, Faculty of Medicine, Firat University, 23119 Elazig, Turkiye. FAU - Kivrak, Tarik AU - Kivrak T AD - Department of Cardiology, Faculty of Medicine, Firat University, 23119 Elazig, Turkiye. FAU - Canleblebici, Mehmet AU - Canleblebici M AD - Department of Ophthalmology, Kayseri State Hospital, 38010, Kayseri Turkiye. FAU - Dal, Ali AU - Dal A AD - Department of Ophthalmology, Faculty of Medicine Mustafa Kemal University, 31060, Hatay, Turkiye. FAU - Balbaba, Mehmet AU - Balbaba M AD - Department of Ophthalmology, Faculty of Medicine, Firat University, 23119 Elazig, Turkiye. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Retina JT - Retina (Philadelphia, Pa.) JID - 8309919 SB - IM OTO - NOTNLM OT - Optical coherence tomography angiography OT - Pulmonary arterial hypertension OT - Retinal microvasculature OT - Vessel density COIS- There are no conflicts of interest or sources of funding associated with the submission for any of the authors. EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 11:04 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 11:04 [entrez] AID - 00006982-990000000-01566 [pii] AID - 10.1097/IAE.0000000000005043 [doi] PST - aheadofprint SO - Retina. 2026 Oct 29. doi: 10.1097/IAE.0000000000005043. PMID- 42954924 OWN - NLM STAT- Publisher LR - 20261029 IS - 1944-8252 (Electronic) IS - 1944-8244 (Linking) DP - 2026 Oct 29 TI - Phage Cocktail-Mediated Ultrasensitive Real-Time Electrochemical Whole-Cell Detection of Klebsiella pneumoniae on a SPCE. LID - 10.1021/acsami.6c14664 [doi] AB - Rapid and selective detection of pathogenic bacteria is essential for timely clinical diagnosis and environmental surveillance. In this work, we report a bacteriophage-assisted electrochemical sensing platform for the selective detection of Klebsiella pneumoniae using an Er2O3/Co3O4/Au/polyethylenimine (PEI)-modified screen-printed carbon electrode (SPCE). The hierarchical nanocomposite was engineered to provide high surface area, enhanced charge transfer kinetics, and abundant functional sites for efficient phage immobilization. PEI served as a cationic linker to facilitate electrostatic attachment of lytic bacteriophages while maintaining their biological activity and host specificity. Upon target recognition, phage-bacteria binding at the electrode interface produced pronounced impedance changes due to interfacial blocking and altered electron transfer dynamics. The developed biosensor exhibited high selectivity for Klebsiella pneumoniae over nontarget bacteria and could distinguish live from dead bacteria. The sensor showed a wide linear detection range of 10-107 CFU/mL with a low detection limit of 7.36 CFU/mL and good repeatability with an RSD of approximately 1.17%. The real-world applicability of the sensor was tested using aquarium water samples and demonstrated good performance, with recovery percentages ranging from approximately 91.71 to 96.26%. This work establishes a robust nano-bio hybrid interface strategy that integrates rare-earth/transition metal oxide electrocatalysts with phage biorecognition on disposable SPCE platforms. The developed system shows strong potential for point-of-care pathogen monitoring in clinical, environmental, and food safety applications. CI - (c) 2026 The Author(s). Published by American Chemical Society. FAU - Richard, Yesurajan Allwin AU - Richard YA AD - Amrita Biosensor Research Lab, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Coimbatore, Tamil Nadu641112, India. AD - Department of Chemistry, Amrita School of Physical Sciences Coimbatore, Amrita Vishwa Vidyapeetham, Coimbatore, Tamil Nadu641112, India. FAU - Hari, Malavika Binitha AU - Hari MB AD - School of Biotechnology Amrita Vishwa Vidyapeetham Kollam, Kerala - 690525, India. FAU - Pradeep, Aarathi AU - Pradeep A AD - Amrita Biosensor Research Lab, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Coimbatore, Tamil Nadu641112, India. AD - Department of Chemistry, Amrita School of Physical Sciences Coimbatore, Amrita Vishwa Vidyapeetham, Coimbatore, Tamil Nadu641112, India. AD - Amrita Biomedical Engineering Centre, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Coimbatore, Tamil Nadu641112, India. FAU - Babu, Pradeesh AU - Babu P AUID- ORCID: 0000-0003-0569-6628 AD - School of Biotechnology Amrita Vishwa Vidyapeetham Kollam, Kerala - 690525, India. FAU - Nair, Bipin AU - Nair B AUID- ORCID: 0000-0002-4944-8805 AD - School of Biotechnology Amrita Vishwa Vidyapeetham Kollam, Kerala - 690525, India. FAU - Kumar, Geetha AU - Kumar G AUID- ORCID: 0000-0002-4584-6929 AD - School of Biotechnology Amrita Vishwa Vidyapeetham Kollam, Kerala - 690525, India. FAU - Gopalakrishnan, Satheesh Babu Thekkedath AU - Gopalakrishnan SBT AUID- ORCID: 0000-0002-9919-4372 AD - Amrita Biosensor Research Lab, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Coimbatore, Tamil Nadu641112, India. AD - Department of Chemistry, Amrita School of Physical Sciences Coimbatore, Amrita Vishwa Vidyapeetham, Coimbatore, Tamil Nadu641112, India. AD - Amrita Biomedical Engineering Centre, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Coimbatore, Tamil Nadu641112, India. LA - eng GR - PDF/2025/006186/LS/Anusandhan National Research Foundation/ PT - Journal Article DEP - 20261029 PL - United States TA - ACS Appl Mater Interfaces JT - ACS applied materials & interfaces JID - 101504991 SB - IM OTO - NOTNLM OT - Klebsiella pneumoniae OT - bacteria detection OT - bacteriophage cocktail OT - electrochemical biosensors OT - electrochemical impedance spectroscopy EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 11:03 PHST- 2026/07/23 00:00 [received] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/22 00:00 [revised] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 11:03 [entrez] AID - 5460944 [pii] AID - 10.1021/acsami.6c14664 [doi] PST - aheadofprint SO - ACS Appl Mater Interfaces. 2026 Oct 29. doi: 10.1021/acsami.6c14664. PMID- 42954919 OWN - NLM STAT- Publisher LR - 20261029 IS - 1466-187X (Electronic) IS - 0142-159X (Linking) DP - 2026 Oct 29 TI - Past-behavioural question-based multiple mini-interviews: A competency-aligned, predictive validity study. PG - 1-9 LID - 10.1080/0142159X.2026.2752868 [doi] AB - INTRODUCTION: Multiple Mini-Interview (MMI) predictive validity studies rarely compare past-behavioural question (PBQ) and situational question (SQ) formats or use competency-aligned workplace-based assessments. This study evaluated which format yielded better predictive validity, and whether a resource-efficient MMI using the superior format demonstrates sustained predictive validity against competency-matched multisource feedback (MSF). METHODS: For Japanese junior trainee recruitment, a rolling, three-station MMI assessed three non-cognitive competencies: patient safety and quality assurance (PSQA), interpersonal network building (INB), and professionalism (Pro). Phase 1 (n = 26) compared PBQ versus SQ predictive validities. Based on Phase 1 results, Phase 2 (n = 65) used the PBQ-only format. Pearson's correlations, deattenuated and corrected for range-restriction, examined relationships between MMI and MSF across overall, and non-cognitive competencies, at year-one and year-two. Furthermore, an exploratory analysis examined correlation between MMI and the cognitive MSF competency, problem solving (PS). RESULTS: Phase 1 established PBQ superiority (corrected r, r(c) = 0.44, p = 0.001) over SQ. In Phase 2, the overall PBQ-based MMI significantly predicted overall year-one MSF (r(c) = 0.52). For the aligned framework, competency-specific correlations were highly significant for PSQA (r(c) = 0.81) and INB (r(c) = 0.60). All the above predictive validities persisted or increased in year-two, whereas Pro demonstrated weak, non-significant correlations at both year-one (r(c) = 0.17), and year-two (r(c) = 0.19). Correlation between overall MMI and MSF PS competency revealed favourable results (r(c) = 0.51 at year-one, persisting in year-two). DISCUSSION: PBQ emerged as the better MMI format over SQ. A resource-efficient, PBQ-based postgraduate MMI produced robust, sustained predictive validity over two years when evaluated against a competency-aligned MSF criterion, alongside an exploratory MSF PS competency. The lack of correlation in the Pro competency underscores the inherent difficulty of baseline assessment, suggesting the need for more professionalism stations. FAU - Yoshimura, Hiroshi AU - Yoshimura H AUID- ORCID: 0009-0001-7411-5164 AD - Okinawa Prefectural Nanbu Medical Centre and Children's Medical Centre, Educational Committee, Okinawa, Japan. FAU - Ponnamperuma, Gominda AU - Ponnamperuma G AUID- ORCID: 0000-0003-0023-6550 AD - Department of Medical Education, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Med Teach JT - Medical teacher JID - 7909593 SB - IM OTO - NOTNLM OT - Competency-specific correlation OT - education, medical, graduate OT - multisource feedback OT - personnel selection OT - positive predictive value EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 11:03 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 11:03 [entrez] AID - 10.1080/0142159X.2026.2752868 [doi] PST - aheadofprint SO - Med Teach. 2026 Oct 29:1-9. doi: 10.1080/0142159X.2026.2752868. PMID- 42954917 OWN - NLM STAT- Publisher LR - 20261029 IS - 1469-0705 (Electronic) IS - 0960-7692 (Linking) DP - 2026 Oct 29 TI - Ultrasonography (IOTA-ADNEX) vs magnetic resonance imaging (ADNEX MR) for diagnosing ovarian cancer in high-risk patients: a prospective study. LID - 10.1002/uog.70368 [doi] AB - OBJECTIVES: To systematically validate and compare ultrasonography, using the ultrasound-based International Ovarian Tumor Analysis - Assessment of Different NEoplasias in the adneXa (IOTA-ADNEX) model, with magnetic resonance imaging (MRI), using the MRI-based ADNEX MR scoring system, as second-line imaging modalities for estimating the risk of adnexal malignancy in patients identified as being at risk of malignancy on first-line ultrasound examination. METHODS: This was a prospective, single-center diagnostic accuracy study designed as a head-to-head comparison. All patients referred to a tertiary referral center at Aarhus University Hospital, Aarhus, Denmark, for suspected adnexal malignancy on first-line imaging between May 2021 and December 2022 were offered second-line diagnostic testing. This comprised both a systematic expert ultrasound examination, including IOTA-ADNEX risk estimation, and a systematic MRI scan with ADNEX MR scores assigned by expert radiologists. The imaging assessments were performed prospectively, with ultrasound examiners and radiologists blinded to the results of other imaging modalities and to the reference standard (tumor outcome). The reference standard was a histopathological diagnosis of the primary adnexal mass or, in a small number of cases, clinical follow-up. Borderline tumors, invasive adnexal malignancy and metastases to the adnexa were grouped as malignancies. Diagnostic performance of the IOTA-ADNEX model, ADNEX MR scoring system and CA 125 for predicting malignancy was evaluated using the area under the receiver-operating-characteristics curve (AUC), and AUCs were compared using DeLong's test. Diagnostic metrics were estimated at clinically relevant cut-offs and scores. Agreement between modalities was assessed for the identification of morphological features and overall risk estimation using Cohen's kappa statistics, intraclass correlation coefficients (ICC) and Bland-Altman plots. For the IOTA-ADNEX model, calibration and the ability to discriminate between tumor subtypes were evaluated. RESULTS: The included cohort comprised 342 patients (342 lesions analyzed). In the final cohort, 78.1% of patients were postmenopausal and the overall prevalence of malignancy was 58.8%. The AUC for distinguishing patients with benign tumors from those with malignant tumors was 0.96 (95% CI, 0.94-0.98) for the IOTA-ADNEX model (with or without CA 125) compared with an AUC of 0.93 (95% CI, 0.91-0.96) for ADNEX MR. Agreement between ultrasound and MRI ranged from moderate to substantial for key morphological features (kappa = 0.46-0.75) and from good to excellent for lesion-size measurements (ICC = 0.78-0.92). The IOTA-ADNEX model performed similarly to the ADNEX MR scoring system regardless of lesion morphology or size. The IOTA-ADNEX model had good calibration. Furthermore, its multinomial discriminative ability between tumor subtypes was generally high, although performance was reduced when distinguishing borderline tumors from Stage-I invasive malignancy (AUC, 0.71 (95% CI, 0.56-0.82)). CONCLUSIONS: The IOTA-ADNEX model and ADNEX MR scoring system demonstrated comparable diagnostic accuracy for differentiating benign from malignant adnexal masses in this study. Results appeared to be consistent when stratified by lesion morphology. Both modalities may be used for second-line assessment of masses deemed at risk of malignancy in the first-line ultrasound examination, depending on resources in specific centers. (c) 2026 The Author(s). Ultrasound in Obstetrics & Gynecology published by John Wiley & Sons Ltd on behalf of International Society of Ultrasound in Obstetrics and Gynecology. CI - (c) 2026 The Author(s). Ultrasound in Obstetrics & Gynecology published by John Wiley & Sons Ltd on behalf of International Society of Ultrasound in Obstetrics and Gynecology. FAU - Dueholm Hjorth, I M AU - Dueholm Hjorth IM AUID- ORCID: 0000-0001-8726-2465 AD - Department of Clinical Medicine, Aarhus University (AU), Aarhus, Denmark. AD - Department of Gynecology and Obstetrics, Aarhus University Hospital (AUH), Aarhus, Denmark. FAU - Ledger, A AU - Ledger A AUID- ORCID: 0009-0003-9769-4597 AD - Department of Development and Regeneration, Katholieke Universiteit Leuven (KU Leuven), Leuven, Belgium. FAU - Moller, C AU - Moller C AD - Department of Gynecology and Obstetrics, Aarhus University Hospital (AUH), Aarhus, Denmark. FAU - Bech, B H AU - Bech BH AUID- ORCID: 0000-0002-0287-3687 AD - Department of Public Health, Aarhus University (AU), Aarhus, Denmark. FAU - Van den Bosch, T AU - Van den Bosch T AUID- ORCID: 0000-0001-6777-5036 AD - Department of Development and Regeneration, Katholieke Universiteit Leuven (KU Leuven), Leuven, Belgium. AD - Department of Gynecology and Obstetrics, University Hospitals Leuven (UZ Leuven), Leuven, Belgium. FAU - Mogensen, O AU - Mogensen O AUID- ORCID: 0000-0001-7655-9909 AD - Department of Clinical Medicine, Aarhus University (AU), Aarhus, Denmark. AD - Department of Gynecology and Obstetrics, Aarhus University Hospital (AUH), Aarhus, Denmark. LA - eng GR - Institut for Klinisk Medicin, Aarhus Universitet/ GR - R302-A17257/Kraeftens Bekaempelse/ GR - Dansk Kraeftforsknings Fond/ PT - Journal Article DEP - 20261029 PL - England TA - Ultrasound Obstet Gynecol JT - Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology JID - 9108340 SB - IM OTO - NOTNLM OT - adnexal disease OT - cancer early diagnosis OT - diagnostic imaging OT - magnetic resonance imaging OT - ovarian cancer OT - ovarian cysts OT - ovarian neoplasms OT - postmenopausal OT - premenopausal OT - ultrasonography EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 11:03 PHST- 2026/07/04 00:00 [revised] PHST- 2025/12/15 00:00 [received] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 11:03 [entrez] AID - 10.1002/uog.70368 [doi] PST - aheadofprint SO - Ultrasound Obstet Gynecol. 2026 Oct 29. doi: 10.1002/uog.70368. PMID- 42954916 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2369-3762 (Electronic) IS - 2369-3762 (Linking) VI - 12 DP - 2026 Oct 29 TI - Construct Validity of Frontier Large Language Models on 4 Spanish Health Professions Specialty-Access Examinations (MIR, EIR, PIR, and FIR): Cross-Sectional Benchmarking and Psychometric Study. PG - e106199 LID - 10.2196/106199 [doi] AB - BACKGROUND: Large language models (LLMs) achieve high scores on health-professions examinations. Whether these scores support the same interpretations as human scores remains uncertain. Spain uses parallel multiple-choice examinations for entry to specialty training in medicine (Medico Interno Residente [MIR]), nursing (Enfermero Interno Residente [EIR]), psychology (Psicologo Interno Residente [PIR]), and pharmacy (Farmaceutico Interno Residente [FIR]). OBJECTIVE: Using the January 2026 MIR, EIR, PIR, and FIR examinations, this study aimed to quantify the correspondence between human and LLM item facility and to describe model accuracy, distractor selection, and performance associated with measured surface features. METHODS: We evaluated 839 valid items using 12 configurations: GPT-5.2 at 3 reasoning efforts and Claude Opus, Sonnet, and Haiku 4.5 (Anthropic), each under neutral and expert prompts, with one run per configuration. The primary measure was the Spearman correlation (rho) between human and machine facility on 828 complete paired items. We examined sensitivity to panel composition, scoring, pooling, and assumed reliability; compared distractor choices with a post hoc plausibility-weighted baseline; and evaluated a surface-feature classifier. Image analyses distinguished supplied from withheld images. An exploratory LLM-judge audit assessed generated explanations. Bootstrap CIs resample items or, for distractor concordance, error events; they exclude run-to-run uncertainty. RESULTS: The highest pooled accuracy was 94.8% (795/839); all 12 configurations answered 668 of 828 paired items correctly (80.7%). Scores clustered near the ceiling among the strongest configurations. Human and machine facility correlated at 0.40 (95% CI 0.34-0.45), with estimates of 0.31 to 0.40 across panel definitions and 0.39 (95% CI 0.33-0.45) after exam-stratified pooling. The observed correlation was lower than predicted by a specified one-axis simulation with a uniform model advantage (0.63; predictive interval 0.59 to 0.66; P<.001). Among 672 model errors, dominant human-distractor selection differed from the plausibility-weighted baseline by -1.25 percentage points (95% CI -4.53 to 2.19), compatible with no difference. Surface-feature accuracy was 34.4% (289/839), similar to selecting the longest option (34.6%, 290/839). The adjusted association with model correctness was uncertain (odds ratio [OR] 0.87, 95% CI 0.72-1.04). Image-associated accuracy was lower both when images were supplied (OR 0.33, 95% CI 0.21-0.53) and when withheld (OR 0.11, 95% CI 0.08-0.16). CONCLUSIONS: In this single 2026 cohort, high LLM scores coexisted with limited separation among the strongest configurations and moderate correspondence with human item difficulty. These findings caution against interpreting model scores as equivalent evidence of human competence. They do not establish clinical performance or invalidate the examinations for human candidates. CI - (c) Clemente Garcia-Hidalgo, Manuel Carpio-Salmeron, Pau Benito, Carlos Carazo-Casas, Beatriz Carratala, Jaime Campos Pavon. Originally published in JMIR Medical Education (https://mededu.jmir.org). FAU - Garcia-Hidalgo, Clemente AU - Garcia-Hidalgo C AUID- ORCID: 0009-0001-6672-2714 AD - Radiology Department, Hospital General Universitario Morales Meseguer, Av Marques de los Velez S/N, Murcia masculine, Murcia, 30008, Spain, 34 666817007. FAU - Carpio-Salmeron, Manuel AU - Carpio-Salmeron M AUID- ORCID: 0009-0009-8772-4565 AD - Endocrinology Department, Santa Lucia University General Hospital, Cartagena, Murcia, Spain. FAU - Benito, Pau AU - Benito P AUID- ORCID: 0000-0002-2480-9133 AD - Department of Preventive Medicine and Epidemiology, Clinical Institute of Medicine and Dermatology (ICMiD), Hospital Clinic de Barcelona, Barcelona, Catalonia, Spain. AD - Barcelona Institute for Global Health (ISGlobal), Universitat de Barcelona, Hospital Clinic de Barcelona, Barcelona, Catalonia, Spain. FAU - Carazo-Casas, Carlos AU - Carazo-Casas C AUID- ORCID: 0000-0001-7568-7140 AD - Department of Otolaryngology, Hospital Universitario Ramon y Cajal, Madrid, Madrid, Spain. FAU - Carratala, Beatriz AU - Carratala B AUID- ORCID: 0009-0000-1751-0048 AD - Innovation and Digital Projects Academic Department, Healthcademia, Madrid, Madrid, Spain. FAU - Campos Pavon, Jaime AU - Campos Pavon J AUID- ORCID: 0000-0001-7852-2675 AD - Academia AMIR, Madrid, Madrid, Spain. AD - Department of Ophthalmology, Hospital Universitario, Madrid, Spain. LA - eng PT - Journal Article DEP - 20261029 PL - Canada TA - JMIR Med Educ JT - JMIR medical education JID - 101684518 SB - IM MH - Humans MH - *Large Language Models MH - Cross-Sectional Studies MH - Spain MH - *Psychometrics/methods/standards MH - *Benchmarking/methods/statistics & numerical data/standards MH - *Educational Measurement/methods/standards/statistics & numerical data MH - Reproducibility of Results OTO - NOTNLM OT - AI OT - Spain OT - clinical competence OT - construct validity OT - educational measurement OT - large language models OT - medical education OT - multiple-choice OT - psychometrics OT - specialty-access examination EDAT- 2026/10/29 17:36 MHDA- 2026/10/29 17:37 CRDT- 2026/10/29 11:03 PHST- 2026/07/03 00:00 [received] PHST- 2026/09/08 00:00 [revised] PHST- 2026/09/10 00:00 [accepted] PHST- 2026/10/29 17:37 [medline] PHST- 2026/10/29 17:36 [pubmed] PHST- 2026/10/29 11:03 [entrez] AID - v12i1e106199 [pii] AID - 10.2196/106199 [doi] PST - epublish SO - JMIR Med Educ. 2026 Oct 29;12:e106199. doi: 10.2196/106199. PMID- 42954911 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1998-3603 (Electronic) IS - 0970-9290 (Linking) VI - 37 IP - 2 DP - 2026 Apr 1 TI - Flapless Piezoelectric Implant Surgery with Photobiomodulation: A Non-Clinical Mechanistic-Bayesian Framework and Study Protocol. PG - 245-249 LID - 10.4103/ijdr.ijdr_234_26 [doi] AB - BACKGROUND: Flapless guided placement, piezoelectric osteotomy, and photobiomodulation (PBM) each have individual biological rationale in implant surgery. Their combined, standardised use has not been prospectively validated. AIM: The aim is to develop a non-clinical, mechanistic-Bayesian framework specifying the intervention, Bayesian prior distributions, and validation strategy for a future randomised trial comparing an integrated flapless piezoelectric PBM protocol against conventional guided flapped rotary placement. MATERIALS AND METHODS: A selective, non-exhaustive narrative review, not a systematic review or formal evidence synthesis, was used to identify representative evidence. No predefined eligibility criteria, database search strategy, or PROSPERO registration was undertaken or is applicable to this article type. 48-hour pain (0-100 mm Visual Analogue Scale) and implant stability quotient (ISQ) at 3 months, both assessed by blinded evaluators. CONCLUSION: The integrated protocol is biologically plausible but hypothesis generating. Prior distributions are derived from selective narrative evidence and carry acknowledged, formally quantified uncertainty, addressed through mandatory pre-specified sensitivity analyses. Pilot and definitive randomised trials are required before clinical adoption. CI - Copyright (c) 2026 Indian Journal of Dental Research. FAU - Dhawan, Pankaj AU - Dhawan P AD - Department of Futuristic Dentistry, School of Dental Sciences, MRIIRS, Faridabad, Haryana, India. FAU - Batra, Puneet AU - Batra P AD - Department of Orthodontics, School of Dental Sciences, MRIIRS, Faridabad, Haryana, India. FAU - Kakar, Ashish AU - Kakar A AD - Department of Futuristic Dentistry, School of Dental Sciences, MRIIRS, Faridabad, Haryana, India. LA - eng PT - Journal Article DEP - 20261028 PL - India TA - Indian J Dent Res JT - Indian journal of dental research : official publication of Indian Society for Dental Research JID - 9202990 RN - 0 (Dental Implants) SB - IM MH - Humans MH - *Low-Level Light Therapy/methods MH - Bayes Theorem MH - *Piezosurgery/methods MH - *Dental Implantation, Endosseous/methods MH - Dental Implants EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 10:55 PHST- 2026/03/27 00:00 [received] PHST- 2026/06/23 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 10:55 [entrez] AID - 01363779-202604000-00025 [pii] AID - 10.4103/ijdr.ijdr_234_26 [doi] PST - ppublish SO - Indian J Dent Res. 2026 Apr 1;37(2):245-249. doi: 10.4103/ijdr.ijdr_234_26. Epub 2026 Oct 28. PMID- 42954902 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1998-3603 (Electronic) IS - 0970-9290 (Linking) VI - 37 IP - 2 DP - 2026 Apr 1 TI - Evaluation of Fibre-Reinforced Composites and Nanohybrid Composites as Post-Endodontic Restorations in Primary Teeth - A Comparative Study. PG - 168-172 LID - 10.4103/ijdr.ijdr_317_26 [doi] AB - BACKGROUND: Fibre-reinforced composites (FRCs) have been introduced to improve the mechanical properties of conventional composite restorations. However, clinical evidence supporting their use in primary teeth remains limited. AIM: To compare the clinical performance of FRCs and nanohybrid composites (NHCs) as post-endodontic restorations in primary teeth over 12 months. MATERIALS AND METHODS: A parallel-arm randomised controlled trial was conducted among 66 children aged 5-9 years requiring pulpectomy in primary molars. Participants were randomly allocated into two groups ( n = 33 each): Group A (FRC) and Group B (NHC). Standardised pulpectomy procedures were followed by placement of the respective experimental restorative materials. Clinical evaluation was performed at baseline, 6 months and 12 months using the modified United States Public Health Service criteria by two calibrated examiners. Intragroup comparisons were analysed using the Friedman test and the Wilcoxon signed-rank test with Bonferroni correction, and intergroup comparisons were performed using the Mann-Whitney U test. RESULTS: Both groups showed comparable baseline scores ( P = 0.18), confirming homogeneity. A statistically significant decline in clinical performance was observed within both groups over time ( P < 0.05). However, no statistically significant differences were found between FRCs and NHCs at baseline, 6 months or 12 months ( P > 0.05). Although slightly greater deterioration was noted in the NHC group, it was not significant. CONCLUSION: Both FRCs and NHCs demonstrated comparable clinical performance over 12 months. Despite improved mechanical properties, FRCs did not show superior clinical outcomes. Restoration success appears to be multifactorial, emphasising the importance of clinical technique and case selection. CI - Copyright (c) 2026 Indian Journal of Dental Research. FAU - Patnana, Arun K AU - Patnana AK AD - Department of Dentistry, All India Institute of Medical Sciences, Rajkot, Gujarat, India. FAU - Narayanan, Divya AU - Narayanan D AD - Department of Dentistry, All India Institute of Medical Sciences, Rajkot, Gujarat, India. FAU - Ali, Rizwan AU - Ali R AD - Department of Dentistry, All India Institute of Medical Sciences, Jodhpur, Rajasthan, India. FAU - Galani, Mohit AU - Galani M AD - Department of Dentistry, All India Institute of Medical Sciences, Rajkot, Gujarat, India. FAU - Karande, Vikram AU - Karande V AD - Department of Dentistry, All India Institute of Medical Sciences, Rajkot, Gujarat, India. FAU - Kumar, Pravin AU - Kumar P AD - Department of Dentistry, All India Institute of Medical Sciences, Jodhpur, Rajasthan, India. LA - eng PT - Comparative Study PT - Journal Article PT - Randomized Controlled Trial DEP - 20261028 PL - India TA - Indian J Dent Res JT - Indian journal of dental research : official publication of Indian Society for Dental Research JID - 9202990 RN - 0 (Composite Resins) RN - 0 (Dental Materials) RN - 0 (fiberglass) RN - 0 (Polymers) RN - 7631-86-9 (Silicon Dioxide) SB - IM MH - Humans MH - *Composite Resins/chemistry/therapeutic use MH - *Tooth, Deciduous MH - Child MH - *Nanocomposites/chemistry MH - Child, Preschool MH - *Dental Restoration, Permanent/methods MH - Pulpectomy/methods MH - Female MH - Male MH - Glass/chemistry MH - Molar MH - *Dental Materials/chemistry MH - Polymers MH - Silicon Dioxide EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 10:55 PHST- 2026/04/27 00:00 [received] PHST- 2026/07/03 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 10:55 [entrez] AID - 01363779-202604000-00009 [pii] AID - 10.4103/ijdr.ijdr_317_26 [doi] PST - ppublish SO - Indian J Dent Res. 2026 Apr 1;37(2):168-172. doi: 10.4103/ijdr.ijdr_317_26. Epub 2026 Oct 28. PMID- 42954901 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1998-3603 (Electronic) IS - 0970-9290 (Linking) VI - 37 IP - 2 DP - 2026 Apr 1 TI - Topical Oxygen Therapy Versus Kenacort in Recurrent Aphthous Stomatitis - A Comparative Study. PG - 163-167 LID - 10.4103/ijdr.ijdr_193_26 [doi] AB - BACKGROUND: Recurrent aphthous stomatitis (RAS) is a common painful oral mucosal disorder causing significant discomfort and functional limitation. Conventional treatments mainly provide symptomatic relief with limited effect on healing. Topical oxygen therapy is a novel, non-invasive modality that may enhance tissue oxygenation and promote mucosal repair. AIM: To evaluate the effectiveness of topical oxygen therapy in reducing ulcer size and pain in patients with RAS and compare its outcomes with Kenacort gel. MATERIALS AND METHODS: Patients with RAS received either topical oxygen therapy or Kenacort gel. Clinical assessments were performed at baseline and follow-up visits. Reduction in ulcer size was the primary outcome, while pain reduction and healing time were secondary outcomes. Statistical significance was set at P < 0.05. RESULTS: Both groups showed significant reductions in ulcer size and pain over time ( P < 0.001). On Day 7, greater ulcer size reduction ( P = 0.07) and lower pain scores ( P = 0.09) were observed in the oxygen therapy group, although differences were not statistically significant. By Day 14, no significant inter-group differences were found in ulcer size ( P = 0.18) or pain scores ( P = 0.15). Complete healing occurred in 70% of the Kenacort group and 77% of the oxygen therapy group ( P = 0.56). CONCLUSION: Topical oxygen therapy showed clinical outcomes comparable to Kenacort and may be considered a safe alternative or adjunctive treatment for RAS. CI - Copyright (c) 2026 Indian Journal of Dental Research. FAU - Jagadeesan, Nithya Devi AU - Jagadeesan ND AD - Department of Oral Medicine and Radiology, Karpaga Vinayaga Institute of Dental Sciences, Affiliated to The Tamil Nadu Dr. MGR Medical University, Chengalpattu, Tamil Nadu, India. FAU - Raj, R R Mahendra AU - Raj RRM FAU - Saravanan, T AU - Saravanan T FAU - Shakila, K R AU - Shakila KR FAU - Bhairavi, D AU - Bhairavi D LA - eng PT - Comparative Study PT - Journal Article DEP - 20261028 PL - India TA - Indian J Dent Res JT - Indian journal of dental research : official publication of Indian Society for Dental Research JID - 9202990 RN - S88TT14065 (Oxygen) SB - IM MH - Humans MH - *Stomatitis, Aphthous/therapy/drug therapy MH - Female MH - Male MH - *Oxygen/administration & dosage/therapeutic use MH - Recurrence MH - Administration, Topical MH - Adult MH - Treatment Outcome MH - Wound Healing MH - Pain Measurement EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 10:55 PHST- 2026/03/09 00:00 [received] PHST- 2026/06/09 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 10:55 [entrez] AID - 01363779-202604000-00008 [pii] AID - 10.4103/ijdr.ijdr_193_26 [doi] PST - ppublish SO - Indian J Dent Res. 2026 Apr 1;37(2):163-167. doi: 10.4103/ijdr.ijdr_193_26. Epub 2026 Oct 28. PMID- 42954897 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1998-3603 (Electronic) IS - 0970-9290 (Linking) VI - 37 IP - 2 DP - 2026 Apr 1 TI - Expression of CDKN2A Gene for Early Detection of Head and Neck Squamous Cell Carcinoma - An Evaluative Study. PG - 134-138 LID - 10.4103/ijdr.ijdr_111_26 [doi] AB - AIM AND OBJECTIVES: Oral squamous cell carcinoma (OSCC) is difficult to detect at an early stage using routine imaging or biopsy. Cyclin-dependent kinase inhibitor 2A ( CDKN2A ) is a major tumour-suppressor gene in OSCC, and alterations in its gene expression reflect early molecular changes during carcinogenesis. This study aims to evaluate CDKN2A gene expression in untreated early-stage OSCC patients and assess its potential relevance for early disease detection. METHODOLOGY: Sixty participants (30 early-stage OSCC patients and 30 matched healthy controls) were included. From OSCC patients, ribonucleic acid (RNA) was isolated from matched tumour and adjacent normal mucosal tissues. CDKN2A gene expression levels were quantified using quantitative reverse-transcription polymerase chain reaction with normalisation to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Human papillomavirus status and clinical features were assessed to interpret biomarker performance. Statistical analysis used the Wilcoxon signed-rank test for paired tissue data, with P < 0.05 considered significant. RESULTS: CDKN2A mRNA expression was significantly lower in OSCC tumour tissue than in adjacent normal mucosa in the present cohort (median 0.65 vs 1.15, P < 0.05). The Cancer Genome Atlas head and neck squamous cell carcinoma (HNSCC) data ( n = 520) showed higher CDKN2A transcript levels in tumours compared with normal tissues. However, oral cavity-specific analysis showed no significant difference ( P = 0.693), which was similar to the findings observed in HPV-negative oral tumours in the present study. CONCLUSION: CDKN2A mRNA is significantly downregulated in early-stage HPV-negative OSCC tissue and may serve as a tissue-based molecular marker. Biofluid CDKN2A mRNA shows low sensitivity for non-invasive screening. Larger cohorts and multi-marker panels may improve its diagnostic performance and clinical utility in early detection. CI - Copyright (c) 2026 Indian Journal of Dental Research. FAU - Gopalakrishnan, Sivakumar AU - Gopalakrishnan S AD - Department of Oral Pathology, Madha Dental College & Hospital, Chennai, Tamil Nadu, India. AD - Research Scholar, Clinical Genetics Lab, Centre for Cellular and Molecular Research, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India. FAU - Jayaseelan, Vijayashree Priyadarshini AU - Jayaseelan VP AD - Research Scientist, Clinical Genetics Lab Centre for Cellular and Molecular Research, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India. FAU - Ramamurthi, Raghini AU - Ramamurthi R AD - Department of Oral Pathology, Madha Dental College & Hospital, Chennai, Tamil Nadu, India. AD - Research Scholar, Clinical Genetics Lab, Centre for Cellular and Molecular Research, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India. LA - eng PT - Journal Article DEP - 20261028 PL - India TA - Indian J Dent Res JT - Indian journal of dental research : official publication of Indian Society for Dental Research JID - 9202990 RN - 0 (Cyclin-Dependent Kinase Inhibitor p16) RN - 0 (Biomarkers, Tumor) RN - 0 (RNA, Messenger) SB - IM MH - Humans MH - *Carcinoma, Squamous Cell/diagnosis/genetics MH - Male MH - Female MH - Middle Aged MH - *Early Detection of Cancer MH - *Head and Neck Neoplasms/genetics/diagnosis MH - *Cyclin-Dependent Kinase Inhibitor p16/genetics MH - Biomarkers, Tumor/genetics MH - Adult MH - *Genes, p16 MH - Aged MH - RNA, Messenger/metabolism MH - *Mouth Neoplasms/genetics/diagnosis EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 10:55 PHST- 2026/02/04 00:00 [received] PHST- 2026/06/04 00:00 [accepted] PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 10:55 [entrez] AID - 01363779-202604000-00003 [pii] AID - 10.4103/ijdr.ijdr_111_26 [doi] PST - ppublish SO - Indian J Dent Res. 2026 Apr 1;37(2):134-138. doi: 10.4103/ijdr.ijdr_111_26. Epub 2026 Oct 28. PMID- 42954877 OWN - NLM STAT- Publisher LR - 20261029 IS - 2724-6116 (Electronic) IS - 2724-6116 (Linking) DP - 2026 Oct 29 TI - 18F-Fluoro-L-dihydroxyphenylalanine positron emission tomography in the initial staging of medullary thyroid carcinoma. LID - 10.23736/S2724-6507.26.04586-0 [doi] AB - BACKGROUND: Accurate initial staging of medullary thyroid carcinoma (MTC) is critical for surgical planning and long-term outcome. While (18)F-Fluorodihydroxyphenylalanine Positron Emission Tomography/Computed Tomography ((18)F-DOPA PET/CT) is well established in the detection of recurrent disease, its role in the preoperative setting remains unclear. This review aimed to evaluate the diagnostic performance and clinical relevance of (18)F-DOPA PET/CT in the initial staging of MTC. METHODS: A systematic literature search was performed to identify studies assessing (18)F-DOPA PET/CT in treatment-naive MTC patients. Four studies met the inclusion criteria and were qualitatively analyzed. Data were synthesized focusing on compartment-specific performance, tumor burden, and potential impact on clinical management. RESULTS: (18)F-DOPA PET/CT demonstrated good sensitivity for primary tumor detection (86-88%), although performance was limited in microcarcinomas. A marked heterogeneity emerged across anatomical compartments. Sensitivity for central lymph node metastases was consistently low (28-53%), whereas higher values were observed in the lateral neck (57-81%), with high specificity when reported. Diagnostic performance was strongly influenced by tumor burden, with significantly reduced sensitivity in low-volume disease and improved detection in patients with more extensive nodal involvement. The integration of biochemical markers further supported a burden-dependent behavior, with higher calcitonin levels associated with improved imaging performance. In distant staging, (18)F-DOPA PET/CT identified metastatic disease in a subset of patients but showed limited sensitivity for small hepatic lesions compared with dedicated MRI. Importantly, improvements in lesion detection did not consistently translate into meaningful changes in surgical management. CONCLUSIONS: The role of (18)F-DOPA PET/CT in the initial staging of MTC is selective and context-dependent. While it may provide incremental value in patients with higher tumor burden or suspected lateral nodal disease, its limited sensitivity in the central compartment and in low-volume disease prevents its use as a standalone staging modality. Therefore, (18)F-DOPA PET/CT should be considered a context-driven adjunct rather than a routine tool, to be integrated within a multimodal imaging strategy rather than used as a standalone staging modality. FAU - Imperiale, Alessio AU - Imperiale A AD - Department of Nuclear Medicine, Strasbourg University Hospital, University of Strasbourg, Strasbourg, France - alessio.imperiale@chru-strasbourg.fr. AD - IPHC, UMR 7178, CNRS, University of Strasbourg, Strasbourg, France - alessio.imperiale@chru-strasbourg.fr. FAU - Albano, Domenico AU - Albano D AD - Department of Nuclear Medicine, ASST Spedali Civili, University of Brescia, Brescia, Italy. FAU - Piccardo, Arnoldo AU - Piccardo A AD - Department of Nuclear Medicine and Thyroid Center, Galliera Hospital, Genoa, Italy. FAU - Paone, Gaetano AU - Paone G AD - Department of Nuclear Medicine, Ente Ospedaliero Cantonale, Bellinzona, Switzerland. AD - Faculty of Biomedical Sciences, Universita della Svizzera Italiana, Lugano, Switzerland. FAU - Huon, Morgan AU - Huon M AD - La Timone University Hospital, Aix-Marseille University, Marseille, France. FAU - Taieb, David AU - Taieb D AD - La Timone University Hospital, Aix-Marseille University, Marseille, France. AD - ERL U1326 "RNAnoTher", Aix-Marseille University, Marseille, France. FAU - Treglia, Giorgio AU - Treglia G AD - Department of Nuclear Medicine, Ente Ospedaliero Cantonale, Bellinzona, Switzerland. AD - Faculty of Biomedical Sciences, Universita della Svizzera Italiana, Lugano, Switzerland. AD - Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland. LA - eng PT - Journal Article DEP - 20261029 PL - Italy TA - Minerva Endocrinol (Torino) JT - Minerva endocrinology JID - 101777342 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 10:44 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 10:44 [entrez] AID - S2724-6507.26.04586-0 [pii] AID - 10.23736/S2724-6507.26.04586-0 [doi] PST - aheadofprint SO - Minerva Endocrinol (Torino). 2026 Oct 29. doi: 10.23736/S2724-6507.26.04586-0. PMID- 42954874 OWN - NLM STAT- Publisher LR - 20261029 IS - 1365-2931 (Electronic) IS - 1364-5706 (Linking) DP - 2026 Oct 29 TI - Beyond weight loss-textbook outcomes, a true benchmark of bariatric care. PG - 1-3 LID - 10.1080/13645706.2026.2730933 [doi] FAU - Lirici, Marco Maria AU - Lirici MM AD - Department of Surgery, Villa Pia International Hospital, Rome, Italy. FAU - Zappa, Marco Antonio AU - Zappa MA AD - Department of Surgery, Villa Maria Hospital (GVM Group), Rimini, Italy. LA - eng PT - Editorial DEP - 20261029 PL - England TA - Minim Invasive Ther Allied Technol JT - Minimally invasive therapy & allied technologies : MITAT : official journal of the Society for Minimally Invasive Therapy JID - 9612996 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 10:42 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 10:42 [entrez] AID - 10.1080/13645706.2026.2730933 [doi] PST - aheadofprint SO - Minim Invasive Ther Allied Technol. 2026 Oct 29:1-3. doi: 10.1080/13645706.2026.2730933. PMID- 42954868 OWN - NLM STAT- Publisher LR - 20261029 IS - 1600-0722 (Electronic) IS - 0909-8836 (Linking) DP - 2026 Oct 29 TI - Corrosion resistance of three types of Co-Cr dental alloys manufactured by casting and selective laser melting. LID - e70163 [pii] LID - 10.1111/eos.70163 [doi] AB - The aim of this study was to investigate the corrosion resistance of three types of Co-Cr alloys manufactured by casting or selective laser melting (SLM). Six Co-Cr dental alloys were classified into three different types according to their formulation (containing Mo, W, or both as alloying elements) and according to the manufacturing process, namely, casting or SLM. Then the electrochemical properties were characterized by Anodic Polarization Scanning (AS) and Electrochemical Impedance Spectroscopy (EIS) in Ringer's and 0.1 M NaCl +0.1 M lactic acid solutions. E(corr), I(corr), E(pit), and corrosion rate (CR) were estimated by AS and statistically analyzed (alpha = 0.05). The EIS data fitted to a Randles circuit. Electrochemical testing revealed statistically significant differences for E(corr) and E(pit) among the alloys tested in the same solution, whereas no differences were identified in I(corr) or CR. The Nyquist plots exhibited semicircular profiles, indicating that the electrochemical response was predominantly controlled by charge transfer processes. For all groups, CR was found much higher in the acidic 0.1 M lactic acid + 0.1 M NaCl solution than in Ringer's one. The results of this study showed that CR depended on the solution rather than alloy type or manufacturing process. CI - (c) 2026 Scandinavian Division of the International Association for Dental Research. Published by John Wiley & Sons Ltd. FAU - Taweel, Sara M Al AU - Taweel SMA AD - Dental Biomaterials Research and Development Chair, College of Dentistry, King Saud University, Riyadh, Saudi Arabia. AD - Prosthetic Dental Sciences Department, College of Dentistry, King Saud University, Riyadh, Saudi Arabia. FAU - Jabbari, Youssef S Al AU - Jabbari YSA AD - Dental Biomaterials Research and Development Chair, College of Dentistry, King Saud University, Riyadh, Saudi Arabia. AD - Prosthetic Dental Sciences Department, College of Dentistry, King Saud University, Riyadh, Saudi Arabia. FAU - Sufyan, Arif AU - Sufyan A AD - Dental Biomaterials Research and Development Chair, College of Dentistry, King Saud University, Riyadh, Saudi Arabia. FAU - Zinelis, Spiros AU - Zinelis S AD - Dental Biomaterials Research and Development Chair, College of Dentistry, King Saud University, Riyadh, Saudi Arabia. AD - Department of Biomaterials, School of Dentistry, National and Kapodistrian University of Athens, Athens, Greece. LA - eng GR - King Saud University/ PT - Journal Article DEP - 20261029 PL - England TA - Eur J Oral Sci JT - European journal of oral sciences JID - 9504563 SB - IM OTO - NOTNLM OT - CAD/CAM OT - Co-Cr-Mo alloys OT - casting OT - corrosion resistance OT - electrochemical properties EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 10:33 PHST- 2026/09/21 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 10:33 [entrez] AID - e70163 [pii] AID - 10.1111/eos.70163 [doi] PST - aheadofprint SO - Eur J Oral Sci. 2026 Oct 29:e70163. doi: 10.1111/eos.70163. PMID- 42954858 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1998-4138 (Electronic) IS - 1998-4138 (Linking) VI - 22 IP - 5 DP - 2026 Jul 1 TI - Malignant proliferating trichilemmal tumor with lymph node metastasis: A rare occurrence. PG - 1026-1029 LID - 10.4103/jcrt.jcrt_1074_25 [doi] AB - Proliferating trichilemmal tumors (PTTs) are rare cutaneous tumors predominantly affecting the head and neck region of elderly women. These tumors are derived from the outer root sheath of the hair follicle and show characteristic trichilemmal keratinization. When the tumor cells invade the surrounding tissue, accompanied by pleomorphism and necrosis, it is described as a malignant proliferating trichilemmal tumor (MPTT). MPTTs are rare, aggressive tumors with only a few cases reported with distant and nodal metastasis in the published literature. We hereby present a rare case of a 51-year-old female with nodal metastasis. CI - Copyright (c) 2026 Journal of Cancer Research and Therapeutics. FAU - Hasan, Zoya AU - Hasan Z AD - Department of Pathology, Maulana Azad Medical College, New Delhi, India. FAU - Mandal, Shramana AU - Mandal S AD - Department of Pathology, Maulana Azad Medical College, New Delhi, India. FAU - Singh, Rajdeep AU - Singh R AD - Department of Surgery, Maulana Azad Medical College, New Delhi, India. FAU - Mallya, Varuna AU - Mallya V AD - Department of Pathology, Maulana Azad Medical College, New Delhi, India. LA - eng PT - Case Reports PT - Journal Article PT - Letter DEP - 20261029 PL - India TA - J Cancer Res Ther JT - Journal of cancer research and therapeutics JID - 101249598 SB - IM MH - Humans MH - Female MH - Lymphatic Metastasis/pathology MH - Middle Aged MH - *Skin Neoplasms/pathology/surgery/diagnosis MH - *Hair Follicle/pathology MH - Cell Proliferation MH - Lymph Nodes/pathology OTO - NOTNLM OT - Invasion OT - malignant proliferating trichilemmal tumor (MPTT) OT - nodal metastasis OT - pleomorphism OT - trichilemmal keratinization EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 10:23 PHST- 2025/05/26 00:00 [received] PHST- 2026/06/03 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 10:23 [entrez] AID - 01363817-202610000-00039 [pii] AID - 10.4103/jcrt.jcrt_1074_25 [doi] PST - ppublish SO - J Cancer Res Ther. 2026 Jul 1;22(5):1026-1029. doi: 10.4103/jcrt.jcrt_1074_25. Epub 2026 Oct 29. PMID- 42954856 OWN - NLM STAT- Publisher LR - 20261029 IS - 1471-9053 (Electronic) IS - 0032-0781 (Linking) DP - 2026 Oct 29 TI - Ca2+-Driven Nanodomain Enrichment And Plasma Membrane Proteome Remodelling Govern Plant Response To Bacterial Outer Membrane Vesicles. LID - pcag147 [pii] LID - 10.1093/pcp/pcag147 [doi] AB - Phytopathogenic outer membrane vesicles (OMVs) can elicit protective immune responses and can be utilised for crop protection. Xanthomonas oryzae pv. oryzae (Xoo), a Gram-negative bacterium that causes Bacterial Blight of rice, releases OMVs. However, the role of Xoo-OMVs in the Xoo-rice interaction remains unexplored. We investigated the early signalling events underlying the plant's response to Xoo-OMV to gain insight into plant-pathogen interactions. We observed that Xoo-OMVs trigger a rapid, unique Ca2+ signal in rice that is essential for evoking an immune response. By imaging and proteomic analysis of the rice plasma membrane (PM), we found that this Ca2+ signal is required for Xoo-OMV-induced nanodomain enrichment and aggregation of defence response-associated proteins in rice PM. The specific protein assembly in the PM helps the plant defend itself against Xoo. In the absence of the early Ca2+ signal, rice cannot respond effectively to Xoo-OMVs, resulting in a compromised immune response. Collectively, our study shows that rice responds to Xoo-OMVs via Ca2+-signal-induced nanodomain assembly and protein reorganisation at the PM, preparing the plant for an imminent pathogen invasion. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Japanese Society of Plant Physiologists. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Mondal, Ishani AU - Mondal I AD - Infectious Diseases and Immunology Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Kolkata-700032, India. FAU - Das, Hrimeeka AU - Das H AD - Infectious Diseases and Immunology Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Kolkata-700032, India. AD - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India. FAU - Behera, Smrutisanjita AU - Behera S AUID- ORCID: 0000-0001-8749-6130 AD - Infectious Diseases and Immunology Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S. C. Mullick Road, Kolkata-700032, India. AD - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India. LA - eng PT - Journal Article DEP - 20261029 PL - Japan TA - Plant Cell Physiol JT - Plant & cell physiology JID - 9430925 SB - IM OTO - NOTNLM OT - Oryza sativa (Rice) OT - Xanthomonas oryzae pv. oryzae (Xoo) OT - Calcium signalling OT - Nanodomain OT - Outer membrane vesicles (OMVs) OT - Plasma membrane EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 10:22 PHST- 2026/06/08 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 10:22 [entrez] AID - 9004506 [pii] AID - 10.1093/pcp/pcag147 [doi] PST - aheadofprint SO - Plant Cell Physiol. 2026 Oct 29:pcag147. doi: 10.1093/pcp/pcag147. PMID- 42954854 OWN - NLM STAT- Publisher LR - 20261029 IS - 2724-6337 (Electronic) IS - 2724-6329 (Linking) DP - 2026 Oct 29 TI - Geriatric dentistry and systemic frailty: diagnostic, surgical, and rehabilitative implications. LID - 10.23736/S2724-6329.26.05305-2 [doi] AB - Population ageing has profoundly changed the clinical management of dental patients, making systemic frailty, multimorbidity and functional dependence key determinants of treatment planning. The aim of this review was to summarize the current evidence on the diagnosis and management of geriatric dental patients, focusing on periodontal disease, implant rehabilitation, oral oncology and the care of non-self-sufficient older adults. A narrative review of the literature was conducted through a comprehensive search of the PubMed/MEDLINE, Scopus and Web of Science databases. International guidelines, systematic reviews, meta-analyses and high-quality clinical studies were preferentially included. The review was developed according to the principles of methodological quality and transparency proposed by the Scale for the Assessment of Narrative Review Articles (SANRA). Representative clinical cases were included to illustrate the practical application of the concepts discussed. Current evidence indicates that chronological age alone should not determine therapeutic decisions. Comprehensive assessment of systemic frailty, functional status, comorbidities and long-term oral health maintenance plays a central role in treatment planning. Personalized, minimally invasive and multidisciplinary approaches appear to improve functional outcomes and oral health-related quality of life, although evidence remains limited for frail and institutionalized older adults. The management of geriatric dental patients should be based on an individualized approach integrating frailty assessment into clinical decision-making. Further prospective studies are needed to develop evidence-based protocols specifically tailored to frail older adults. Integrating frailty assessment into routine dental practice may facilitate personalized treatment planning, optimize oral rehabilitation and improve long-term oral health-related quality of life in older patients. FAU - Padova, Silvia AU - Padova S AD - Department of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy. AD - Dental Clinic, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy. FAU - Carini, Fabio AU - Carini F AD - Department of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy - fabiocarini4@gmail.com. AD - Dental Clinic, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy. FAU - Garatti, Barbara AU - Garatti B AD - Department of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy. AD - Dental Clinic, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy. FAU - Baldoni, Marco AU - Baldoni M AD - Department of Medicine and Surgery, University of Milano-Bicocca, Monza, Italy. AD - Dental Clinic, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy. LA - eng PT - Journal Article DEP - 20261029 PL - Italy TA - Minerva Dent Oral Sci JT - Minerva dental and oral science JID - 101778009 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 10:16 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 10:16 [entrez] AID - S2724-6329.26.05305-2 [pii] AID - 10.23736/S2724-6329.26.05305-2 [doi] PST - aheadofprint SO - Minerva Dent Oral Sci. 2026 Oct 29. doi: 10.23736/S2724-6329.26.05305-2. PMID- 42954842 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1948-7185 (Electronic) IS - 1948-7185 (Linking) VI - 17 IP - 43 DP - 2026 Oct 29 TI - Strain-Gradient Engineering of Topological, Nonlinear Optical, and Spin Chiral Responses in MnBi2Te4. PG - 12200-12207 LID - 10.1021/acs.jpclett.6c02756 [doi] AB - Strain gradients arising from nonuniform interlayer expansion in layered van der Waals materials simultaneously modulate interlayer coupling and break inversion symmetry, yet their potential as a control knob for topological quantum materials remains unexplored. Using first-principles calculations, we demonstrate that graded interlayer expansion characterized by an effective gap-strain gradient in five-septuple-layer MnBi2Te4 drives three distinct physical responses: a topological phase transition from a quantum anomalous Hall state (C = 1) to a trivial insulator (C = 0), a second-harmonic-generation response in the mid-to-far-infrared spectral range, and a Rashba-type chiral spin texture with opposite inner-outer helicities. All three responses share a common microscopic origin in the layer-selective redistribution of interlayer hybridization, which weakens the Bi-Te orbital inversion near the Gamma point and generates an out-of-plane polar field. Our findings establish strain-gradient engineering as a design principle for coordinating topological, nonlinear optical, and spin-textural functionalities in layered quantum materials. CI - (c) 2026 American Chemical Society. FAU - Yan, Hongtao AU - Yan H AD - School of Advanced Materials and Nanotechnology, Xidian University, Xi'an710126, P. R. China. FAU - Jia, Dongliang AU - Jia D AD - School of Advanced Materials and Nanotechnology, Xidian University, Xi'an710126, P. R. China. FAU - Zhang, Bolun AU - Zhang B AD - School of Advanced Materials and Nanotechnology, Xidian University, Xi'an710126, P. R. China. FAU - Guo, Xinming AU - Guo X AD - School of Advanced Materials and Nanotechnology, Xidian University, Xi'an710126, P. R. China. FAU - Tan, Dan AU - Tan D AUID- ORCID: 0000-0002-6148-7751 AD - School of Advanced Materials and Nanotechnology, Xidian University, Xi'an710126, P. R. China. AD - School of Physics, Xidian University, Xi'an710126, P. R. China. LA - eng GR - 12674022/National Natural Science Foundation of China/ GR - 2025JC-YBQN-016/Natural Science Basic Research Program of Shaanxi Province/ GR - GS2025MS018/Beijing Key Laboratory of High-Entropy Energy Materials and Devices, Beijing Institute of Nanoenergy and Nanosystems/ PT - Journal Article PL - United States TA - J Phys Chem Lett JT - The journal of physical chemistry letters JID - 101526034 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 10:14 PHST- 2026/08/16 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 10:14 [entrez] AID - 5453402 [pii] AID - 10.1021/acs.jpclett.6c02756 [doi] PST - ppublish SO - J Phys Chem Lett. 2026 Oct 29;17(43):12200-12207. doi: 10.1021/acs.jpclett.6c02756. PMID- 42954825 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1948-7185 (Electronic) IS - 1948-7185 (Linking) VI - 17 IP - 43 DP - 2026 Oct 29 TI - A Dual Vibrational Probe Framework for Studying Solvation in Highly Heterogeneous Molecular Environments. PG - 12382-12389 LID - 10.1021/acs.jpclett.6c02383 [doi] AB - Vibrational probes are essential for studying interactions and dynamics of complex environments, but they often rely on computer simulations to describe the system. Here, a dual-IR-probe approach based on the alkyne molecular framework is demonstrated for two probes: MOC (low polarity) and TFPB (nonpolar). In these dual-probes, the secondary IR-active group allows their precise localization, while the alkyne group is used to probe the dynamics. The approach is validated in two complex liquids: a deep eutectic solvent (DES) and a water micelle. In the DES, the dual-IR scheme experimentally locates MOC in one specific domain. In water micelles, MOC and TFPB localize selectively at the interface and within the hydrophobic core, respectively, providing an independent characterization of the two environments. The results demonstrate that the alkyne-based dual-probe is a versatile molecular framework for characterizing the structure, interactions, and dynamics of complex liquids using only experimental methods. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Deshmukh, Samadhan H AU - Deshmukh SH AUID- ORCID: 0000-0002-0326-6125 AD - Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana70803, United States. FAU - Sepulveda-Montano, Laura X AU - Sepulveda-Montano LX AUID- ORCID: 0000-0001-9538-4032 AD - Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana70803, United States. FAU - Kuroda, Daniel G AU - Kuroda DG AUID- ORCID: 0000-0002-4752-7024 AD - Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana70803, United States. LA - eng GR - DE-SC0025202/Basic Energy Sciences/ PT - Journal Article PL - United States TA - J Phys Chem Lett JT - The journal of physical chemistry letters JID - 101526034 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 10:14 PHST- 2026/07/16 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 10:14 [entrez] AID - 5451138 [pii] AID - 10.1021/acs.jpclett.6c02383 [doi] PST - ppublish SO - J Phys Chem Lett. 2026 Oct 29;17(43):12382-12389. doi: 10.1021/acs.jpclett.6c02383. PMID- 42954816 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1365-2060 (Electronic) IS - 0785-3890 (Linking) VI - 58 IP - 1 DP - 2026 Dec TI - Clinical, metabolic, dermoscopic, and reflectance confocal microscopy features of prurigo pigmentosa: a retrospective case series. PG - 2751276 LID - 10.1080/07853890.2026.2751276 [doi] AB - BACKGROUND: Prurigo pigmentosa (PP) is a chronic, recurrent inflammatory dermatitis with nonspecific stage-dependent clinical features, leading to misdiagnosis. Noninvasive imaging may complement clinical and histopathological assessment. Metabolic abnormalities have been reported in PP, although their clinical significance remains unclear. OBJECTIVES: To characterize the clinical, metabolic, dermoscopic, and reflectance confocal microscopy (RCM) features of PP and reduce misdiagnosis. METHODS: We retrospectively reviewed patients with histopathologically confirmed PP and available metabolic screening data from July 2020 to April 2026. Clinical, dermoscopic, RCM, laboratory, treatment, and follow-up data were extracted from medical records. RESULTS: Nine women aged 17-33 years had seasonal eruptions (June-September). Seven reported dietary triggers, including caloric restriction in four and ketogenic diets in three. Disease duration ranged from 0.5 to 37 months, with initial misdiagnoses as eczema or contact dermatitis. Metabolic abnormalities included dyslipidemia (n=8), type 2 diabetes (n=3), elevated urinary ketones (n=3), hepatic steatosis (n=7), and elevated body mass index (n=7). Lesions were classified as early (n=3), fully developed (n=5), and late stage (n=1). Dermoscopy identified punctate, linear, and reticular vascular patterns with granular, patchy, or honeycomb-like brown pigmentation. RCM revealed epidermal spongiosis, basal cell degeneration, melanophage infiltration, and dermal inflammatory infiltrates. CONCLUSION: Nonspecific features and coexistence of lesions at different stages contribute to diagnostic difficulty. Dermoscopy and RCM may provide complementary noninvasive information for clinical and histopathological evaluation. Metabolic screening may be considered in patients with recurrent disease, dietary triggers, elevated BMI, ketonuria, or suspected lipid/glucose metabolism abnormalities. FAU - Tan, Meng-Jun AU - Tan MJ AUID- ORCID: 0009-0005-8409-8483 AD - Department of Dermatology, Yijishan Hospital, the First Affiliated Hospital of Wannan Medical University, Wuhu, China. FAU - Chang, Xiao-Li AU - Chang XL AD - Department of Dermatology, Yijishan Hospital, the First Affiliated Hospital of Wannan Medical University, Wuhu, China. FAU - Wang, Sai-Nan AU - Wang SN AD - Department of Dermatology, Yijishan Hospital, the First Affiliated Hospital of Wannan Medical University, Wuhu, China. FAU - Wang, Fan AU - Wang F AD - Department of Dermatology, Yijishan Hospital, the First Affiliated Hospital of Wannan Medical University, Wuhu, China. FAU - Zhang, Wang-Yu AU - Zhang WY AD - Department of Dermatology, Yijishan Hospital, the First Affiliated Hospital of Wannan Medical University, Wuhu, China. FAU - Xiao-Hong, Lu AU - Xiao-Hong L AD - Department of Dermatology, Yijishan Hospital, the First Affiliated Hospital of Wannan Medical University, Wuhu, China. FAU - Qiang, Di AU - Qiang D AD - Department of Dermatology, Yijishan Hospital, the First Affiliated Hospital of Wannan Medical University, Wuhu, China. FAU - Tao, Yuan AU - Tao Y AUID- ORCID: 0000-0002-0604-3437 AD - Department of Dermatology, Yijishan Hospital, the First Affiliated Hospital of Wannan Medical University, Wuhu, China. FAU - He, Cai-Feng AU - He CF AUID- ORCID: 0000-0002-9833-8186 AD - Department of Dermatology, Yijishan Hospital, the First Affiliated Hospital of Wannan Medical University, Wuhu, China. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Ann Med JT - Annals of medicine JID - 8906388 SB - IM MH - Humans MH - Female MH - Retrospective Studies MH - *Prurigo/diagnosis/pathology/metabolism/diagnostic imaging MH - Adult MH - Microscopy, Confocal/methods MH - Adolescent MH - Young Adult MH - *Dermoscopy/methods MH - Skin/pathology/diagnostic imaging OAB - Frequent coexisting metabolic abnormalities were observed in this selected case series of patients with PP.Dermoscopy and reflectance confocal microscopy may support earlier recognition of stage-specific changes, while metabolic assessment may be considered in patients with relevant dietary or metabolic risk factors. OABL- eng OTO - NOTNLM OT - Prurigo pigmentosa OT - dermoscopy OT - histopathology OT - metabolic dysregulation OT - reflectance confocal microscopy EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 10:14 PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 10:14 [entrez] AID - 10.1080/07853890.2026.2751276 [doi] PST - ppublish SO - Ann Med. 2026 Dec;58(1):2751276. doi: 10.1080/07853890.2026.2751276. Epub 2026 Oct 29. PMID- 42954810 OWN - NLM STAT- Publisher LR - 20261029 IS - 1744-7593 (Electronic) IS - 1742-5247 (Linking) DP - 2026 Oct 29 TI - An update on combinational chemo-immunotherapy for breast cancer using nanotechnology. LID - 10.1080/17425247.2026.2749964 [doi] AB - INTRODUCTION: Breast cancer is still a considerable public health issue worldwide. However, earlier studies reported that chemotherapeutic drugs suppress the immune system. Therefore, a combination of chemotherapeutic agents with an immunomodulator might enhance antitumor immunity through immunogenic cell death, cross-priming, and the regulation of immune checkpoint molecules. AREA COVERED: Nanotechnology-enabled drug delivery has received considerable attention in chemo-immunotherapy. A comprehensive literature review was performed using google scholar, Science Direct, the Clinical Research Database, PubMed/MEDLINE, Scopus, Web of Science, and DOAJ. The review discusses the combination of immunotherapy and nanotechnology including AI-based techniques used to identify suitable biomarkers and to enable selective drug delivery to breast cancer cells. EXPERT OPINION: The combinational therapy containing chemotherapeutic agents and immunomodulators has shown promising results due to their synergistic effects. The application of artificial intelligence (AI) in nanomedicine has enabled the design of nanoparticles, predict their interactions with drugs, and personalize treatment based on patients' individual tumor profiles. This advancement has marked the advent of precision medicine in oncology, which focuses on tailoring therapies to each tumor's molecular and genetic profile. Despite these encouraging developments, several challenges remain in nanotechnology for cancer therapy, including mass production, biosafety, regulatory issues, and the successful translation of laboratory findings into clinical practice. FAU - Saraf, Shivani AU - Saraf S AD - Department of Pharmaceutical Sciences, Dr. Harisingh Gour University, Sagar, India. AD - Babulal Tarabai Institute of Pharmaceutical Science, Sagar, India. FAU - Tiwari, Ankita AU - Tiwari A AD - School of Pharmacy, Sharda University, Greater Noida, India. FAU - Kumari, Priyanka AU - Kumari P AUID- ORCID: 0000-0003-2670-7437 AD - Department of Pharmaceutical Technology, NSHM Knowledge Campus, Kolkata - Group of Institutions, Kolkata, India. FAU - Gautam, Laxmikant AU - Gautam L AD - Department of Pharmaceutical Sciences, Dr. Harisingh Gour University, Sagar, India. AD - Babulal Tarabai Institute of Pharmaceutical Science, Sagar, India. FAU - Jain, Sanjay K AU - Jain SK AUID- ORCID: 0000-0002-9241-3114 AD - Department of Pharmaceutical Sciences, Dr. Harisingh Gour University, Sagar, India. AD - Babulal Tarabai Institute of Pharmaceutical Science, Sagar, India. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - Expert Opin Drug Deliv JT - Expert opinion on drug delivery JID - 101228421 SB - IM OTO - NOTNLM OT - Breast cancer OT - artificial intelligence OT - chemotherapy OT - immunotherapy OT - nanotechnology EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 10:12 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 10:12 [entrez] AID - 10.1080/17425247.2026.2749964 [doi] PST - aheadofprint SO - Expert Opin Drug Deliv. 2026 Oct 29. doi: 10.1080/17425247.2026.2749964. PMID- 42954807 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2561-326X (Electronic) IS - 2561-326X (Linking) VI - 10 DP - 2026 Oct 20 TI - Dataset-Agnostic Biological Age Calculator and Predictions of Mortality in NHANES (1999-2010), UK Biobank (2006-2010), and Dalian Heart Failure (2015-2023) Cohorts: Development and Validation Study. PG - e95321 LID - 10.2196/95321 [doi] AB - BACKGROUND: A positive difference between biological age (BA) and chronological age indicates accelerated aging. The application of existing BA models is limited by high technical demands, a lack of alignment across diverse populations, and difficulty in updating them to accommodate expanding cohorts. OBJECTIVE: To address the issues in existing tools, this project aims to develop and validate an automated toolkit for adaptive BA estimation. METHODS: The proposed toolkit, named BACalc, facilitates the processing of raw clinical datasets stored in CSV format. Within the graphical user interface, users can select (1) the chronological age column, (2) binary variables (eg, sex, smoking, and alcohol use), and (3) continuous variables (eg, creatinine levels) for BA estimation, using the principal component analysis (PCA) method with the Dubina correction or the Klemera-Doubal method (KDM). All numerical operations are implemented using the NumPy and Pandas libraries, and linear modeling components are provided by scikit-learn and SciPy. The program generates three main output files: (1) the row-wise predictions, (2) the regression statistics, and (3) the explicit linear equations and PCA loadings. Example outputs were generated using the National Health and Nutrition Examination Survey (NHANES, 1999-2010), UK Biobank (2006-2010), and Dalian Heart Failure (2015-2023). The results were compared with the reference BioAge R package. RESULTS: Using the KDM model, the BACalc-derived and BioAge-derived estimates exhibit a near-perfect correlation (r=0.9937, mean absolute error 2.02 y). The PCA-Dubina model also shows a strong correlation with BioAge (r=0.8871), within a +/-20-year window of accelerated aging residuals. In the UK Biobank, a 1-year increase in PCA-Dubina-derived age acceleration was associated with a 1.77% higher hazard of all-cause mortality (hazard ratio [HR] 1.018, 95% CI 1.017-1.018; P<.001). Similarly, KDM-derived age acceleration was associated with a 1.59% higher hazard of mortality per year of accelerated aging (HR 1.016, 95% CI 1.016-1.016; P<.001). In the Dalian cohort, a 1-year increase in age acceleration derived from the PCA-Dubina model (BAc) independently predicted mortality with a 2.84% increased hazard per year (HR 1.028, 95% CI 1.022-1.035; P<.001). Similarly, KDM-derived age acceleration (BA_EC) was associated with a 1.26% increased hazard of mortality per year of accelerated aging (HR 1.013, 95% CI 1.007-1.018; P<.001). CONCLUSIONS: BACalc provides a reliable platform for BA estimation, consistent with previously validated computational frameworks and convenient for real-time updating. BA acceleration predicted mortality in 2 independent cohorts. CI - (c) Haipeng Liu, Angelina Amabi Perez Aranda, Anselm Au, Derek Wu, Haixin Zhou, Wing Tak Wong, Tong Liu, Mehrdad Shahmohammadi Beni, Gary Tse. Originally published in JMIR Formative Research (https://formative.jmir.org). FAU - Liu, Haipeng AU - Liu H AUID- ORCID: 0000-0002-4212-2503 AD - Cardiovascular Analytics Group, Kowloon, China (Hong Kong). FAU - Amabi Perez Aranda, Angelina AU - Amabi Perez Aranda A AUID- ORCID: 0009-0000-7598-0435 AD - School of Nursing and Health Sciences, Hong Kong Metropolitan University, 1 Sheung Shing Street, Ho Man Tin, Kowloon, China (Hong Kong), +852 3970 2942. FAU - Au, Anselm AU - Au A AUID- ORCID: 0009-0008-9228-8959 AD - School of Nursing and Health Sciences, Hong Kong Metropolitan University, 1 Sheung Shing Street, Ho Man Tin, Kowloon, China (Hong Kong), +852 3970 2942. FAU - Wu, Derek AU - Wu D AUID- ORCID: 0009-0005-9948-1071 AD - Department of Biomedical Engineering and Health Sciences, McMaster University, Hamilton, ON, Canada. FAU - Zhou, Haixin AU - Zhou H AUID- ORCID: 0009-0001-9146-4518 AD - School of Nursing and Health Sciences, Hong Kong Metropolitan University, 1 Sheung Shing Street, Ho Man Tin, Kowloon, China (Hong Kong), +852 3970 2942. FAU - Wong, Wing Tak AU - Wong WT AUID- ORCID: 0000-0002-4514-3780 AD - School of Life Sciences, Chinese University of Hong Kong, Shatin, China (Hong Kong). FAU - Liu, Tong AU - Liu T AUID- ORCID: 0000-0003-0482-0738 AD - Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular disease, Department of Cardiology, Tianjin Institute of Cardiology, Second Hospital of Tianjin Medical University, Tianjin, China. FAU - Beni, Mehrdad Shahmohammadi AU - Beni MS AUID- ORCID: 0009-0006-4736-0670 AD - School of Nursing and Health Sciences, Hong Kong Metropolitan University, 1 Sheung Shing Street, Ho Man Tin, Kowloon, China (Hong Kong), +852 3970 2942. FAU - Tse, Gary AU - Tse G AUID- ORCID: 0000-0001-5510-1253 AD - School of Nursing and Health Sciences, Hong Kong Metropolitan University, 1 Sheung Shing Street, Ho Man Tin, Kowloon, China (Hong Kong), +852 3970 2942. LA - eng PT - Journal Article PT - Validation Study DEP - 20261020 PL - Canada TA - JMIR Form Res JT - JMIR formative research JID - 101726394 SB - IM MH - Humans MH - Female MH - UK Biobank MH - Male MH - Aged MH - United Kingdom/epidemiology MH - *Heart Failure/mortality MH - Nutrition Surveys/statistics & numerical data MH - Middle Aged MH - *Aging/physiology MH - Cohort Studies MH - *Mortality/trends MH - United States MH - Adult OTO - NOTNLM OT - KDM OT - Klemera-Doubal method OT - PCA OT - biological age OT - numerical analysis OT - open-source model OT - principal component analysis EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 10:03 PHST- 2026/03/14 00:00 [received] PHST- 2026/05/21 00:00 [revised] PHST- 2026/05/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 10:03 [entrez] AID - v10i1e95321 [pii] AID - 10.2196/95321 [doi] PST - epublish SO - JMIR Form Res. 2026 Oct 20;10:e95321. doi: 10.2196/95321. PMID- 42954798 OWN - NLM STAT- Publisher LR - 20261029 IS - 1029-046X (Electronic) IS - 1026-776X (Linking) DP - 2026 Oct 29 TI - Integrating QSAR, molecular dynamics and MM-GBSA to re-rank natural product-derived DPP4 candidates from virtual screening. PG - 1-31 LID - 10.1080/1062936X.2026.2752983 [doi] AB - Dipeptidyl peptidase-4 (DPP4) is a validated target for type 2 diabetes, and virtual screening of natural product libraries is a common route to new chemotypes. We asked whether a top docking rank survives independent scrutiny. From COCONUT, 85,600 compounds were filtered to 32,008 and docked against DPP4; 873 outscored the crystallographic ligand N7F (-10.01 kcal/mol), with Lig_771 first at -12.5 kcal/mol. Across four 300 ns simulations (two replicas per complex, 1.2 mus) Lig_771 stayed bound but never reproduced the gliptin recognition motif: the control held its salt bridges to Glu205 and Glu206 in over 93% of frames, whereas Lig_771 formed no persistent hydrogen bond to these residues and sampled two distinct poses. MM-GBSA gave -15.6 kcal/mol for Lig_771 against -23.9 for the control. A QSAR model built on 4327 measured DPP4 inhibitors (scaffold-based external R(2) = 0.415; random split 0.669) predicted a pIC(50) of 6.59 for Lig_771, below linagliptin (8.29), sitagliptin (7.39) and vildagliptin (6.96). None of the three analyses corroborated the docking ranking. The study is computational and does not establish DPP4 inhibition. Lig_771 occupies largely unexplored chemical space (maximum Tanimoto similarity 0.316) and merits testing, but selecting hits on docking score alone would have been misleading here. FAU - Aktas, E AU - Aktas E AUID- ORCID: 0000-0002-9422-3402 AD - Faculty of Art and Science, Molecular Biology and Genetics, Yildiz Technical University, Istanbul, Turkiye. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - SAR QSAR Environ Res JT - SAR and QSAR in environmental research JID - 9440156 SB - IM OTO - NOTNLM OT - DPP4 inhibition OT - density functional theory OT - drug discovery OT - machine learning OT - molecular docking and molecular dynamics simulation OT - natural products virtual screening EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 10:03 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 10:03 [entrez] AID - 10.1080/1062936X.2026.2752983 [doi] PST - aheadofprint SO - SAR QSAR Environ Res. 2026 Oct 29:1-31. doi: 10.1080/1062936X.2026.2752983. PMID- 42954794 OWN - NLM STAT- Publisher LR - 20261029 IS - 2576-6422 (Electronic) IS - 2576-6422 (Linking) DP - 2026 Oct 29 TI - Synergistic Berberine/Pirfenidone-Loaded Thermosensitive Hydrogel: Integrated Antibacterial Defense Activity and Pirfenidone-Mediated Angiogenesis for Efficient Diabetic Wound Repair. LID - 10.1021/acsabm.6c01837 [doi] AB - Diabetic infected wounds (DIWs) represent a global healthcare burden owing to impaired angiogenesis and persistent infections. Conventional interventions typically target a single pathogenic factor, and the short residence time of free drugs within the wound bed further limits their bioavailability. In this study, we constructed an injectable thermosensitive hydrogel (CPP) based on poloxamer and chitosan, coloading berberine (BBR) and pirfenidone (PFD) to achieve combinatorial antibacterial and proangiogenic functions. CPP@(BBR + PFD) achieved sustained dual-drug release, with the gel matrix showing prolonged residence at the injection site and reduced systemic exposure. Mechanistically, BBR eliminated pathogenic bacteria, an activity previously attributed to disruption of bacterial membrane integrity, thereby removing the infectious initiator of the inflammatory cascade. PFD concurrently inhibited the inflammatory response, promoted angiogenesis and collagen deposition, and accelerated the progression from inflammation to tissue remodeling. CPP@(BBR + PFD) significantly accelerated wound closure (89.2% +/- 1.9% on day 13), reduced bacterial burden, and improved tissue regeneration quality. Our findings demonstrate that rationally engineered multifunctional hydrogels can overcome pathological barriers to diabetic wound repair, offering a minimally invasive and efficient therapeutic platform for chronic diabetic wounds. CI - (c) 2026 American Chemical Society. FAU - Cheng, Peijie AU - Cheng P AD - Changchun University of Science and Technology , Changchun, Jilin130022, China. FAU - Li, Jinyang AU - Li J AD - Changchun University of Science and Technology , Changchun, Jilin130022, China. FAU - Wang, Qingshuang AU - Wang Q AD - Changchun University of Science and Technology , Changchun, Jilin130022, China. FAU - Tang, Ziqiao AU - Tang Z AD - Changchun University of Science and Technology , Changchun, Jilin130022, China. FAU - Xu, Jiaze AU - Xu J AD - Changchun University of Science and Technology , Changchun, Jilin130022, China. FAU - Cheng, Ziting AU - Cheng Z AD - Changchun University of Science and Technology , Changchun, Jilin130022, China. FAU - Jiang, Xue AU - Jiang X AD - Changchun University of Science and Technology , Changchun, Jilin130022, China. FAU - Zhang, Zhe AU - Zhang Z AUID- ORCID: 0000-0001-6190-5215 AD - School of Biological and Chemical Engineering, Panzhihua University, Panzhihua, Sichuan617000, China. FAU - Feng, Xiangru AU - Feng X AUID- ORCID: 0000-0001-7671-3144 AD - Changchun University of Science and Technology , Changchun, Jilin130022, China. LA - eng GR - 2026YFHZ0347/Science and Technology Department of Sichuan Province/ GR - JJKH20261547KJ/Education Department of Jilin Province/ GR - 20250301039YY/Jilin Provincial Scientific and Technological Development Program/ PT - Journal Article DEP - 20261029 PL - United States TA - ACS Appl Bio Mater JT - ACS applied bio materials JID - 101729147 SB - IM OTO - NOTNLM OT - angiogenesis OT - berberine OT - diabetic infected wound OT - pirfenidone OT - thermo-sensitive hydrogel EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 10:03 PHST- 2026/08/25 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 10:03 [entrez] AID - 5460768 [pii] AID - 10.1021/acsabm.6c01837 [doi] PST - aheadofprint SO - ACS Appl Bio Mater. 2026 Oct 29. doi: 10.1021/acsabm.6c01837. PMID- 42954780 OWN - NLM STAT- Publisher LR - 20261029 IS - 1460-9592 (Electronic) IS - 1155-5645 (Linking) DP - 2026 Oct 29 TI - Fidget and Sensory Toys for Anxiety and Emotional Regulation in Children: A Scoping Review With Implications for Pediatric Perioperative Care. LID - 10.1002/pan.70327 [doi] AB - BACKGROUND: Non-pharmacological interventions are essential to reduce procedural anxiety in pediatric care. Fidget and sensory toys are widely used for distraction and self-regulation, yet evidence on their effectiveness remains fragmented. This scoping review mapped the evidence on fidget and sensory toys across general pediatric settings, with a focus on perioperative outcomes-such as anxiety, distress, and pain-to highlight relevant gaps in care. METHODS: This scoping review followed the Joanna Briggs Institute (JBI) methodology and the PRISMA-ScR guidelines. Searches were conducted in MEDLINE/PubMed, EMBASE, Scopus, Web of Science, and Cochrane CENTRAL, complemented by trial registries, gray literature, citation tracking, and reference screening. Studies involving children (0-18 years) evaluating fidget or sensory toys in any setting were eligible. Two independent reviewers performed study selection and data extraction. FINDINGS: Fifteen studies published between 2017 and 2026 met the inclusion criteria. Fidget spinners were the most frequently investigated device (n = 9), followed by stress balls (n = 3). Studies were conducted in educational (n = 8) and healthcare settings (n = 7). Educational studies generally reported limited or no benefits for attention, engagement, behavior, or academic performance. In contrast, healthcare studies generally suggested reductions in anxiety, pain, fear, and procedural distress, although findings were heterogeneous, and outcome measures varied substantially. CONCLUSIONS: Current evidence does not support the routine use of fidget toys to improve attention or academic performance in educational settings. Evidence from healthcare and procedural settings suggests that fidget and sensory toys may be feasible distraction tools for selected pediatric patients, but evidence is insufficient to support routine perioperative use. Further high-quality randomized controlled trials are needed to establish standardized intervention protocols and identify the pediatric populations and clinical settings most likely to benefit. CI - (c) 2026 The Author(s). Pediatric Anesthesia published by John Wiley & Sons Ltd. FAU - Pereira, Lucas Ferreira Gomes AU - Pereira LFG AUID- ORCID: 0000-0002-7802-8877 AD - Discipline of Anesthesiology, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, Brazil. AD - Department of Pediatrics, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, Brazil. FAU - Quintao, Vinicius Caldeira AU - Quintao VC AUID- ORCID: 0000-0001-6459-7983 AD - Discipline of Anesthesiology, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, Brazil. AD - Department of Pediatrics, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, Brazil. AD - Instituto da Crianca e Do Adolescente, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, Brazil. FAU - Duarte, Nicolle de Souza AU - Duarte NS AUID- ORCID: 0000-0002-1681-6261 AD - Discipline of Anesthesiology, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, Brazil. AD - Instituto da Crianca e Do Adolescente, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, Brazil. FAU - Carlos, Ricardo Vieira AU - Carlos RV AUID- ORCID: 0000-0002-5787-8979 AD - Discipline of Anesthesiology, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, Brazil. AD - Instituto da Crianca e Do Adolescente, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, Brazil. FAU - Pereira, Julia Ferreira Gomes AU - Pereira JFG AUID- ORCID: 0000-0003-2170-0594 AD - Department of Medicine, University of Taubate, Taubate, Brazil. FAU - de Souza, Guilherme Silva Hirata AU - de Souza GSH AUID- ORCID: 0009-0002-8482-3874 AD - Department of Medicine, University of Taubate, Taubate, Brazil. FAU - von Ungern-Sternberg, Britta S AU - von Ungern-Sternberg BS AUID- ORCID: 0000-0002-8043-8541 AD - Department of Anaesthesia and Pain Medicine, Perth Children's Hospital, Child and Adolescent Health Service, Nedlands, Western Australia, Australia. AD - Anaesthesia and Pain Management Program, Institute for Paediatric Perioperative Excellence, the University of Western Australia, Perth, Western Australia, Australia. AD - Perioperative Medicine Team, The Kids Research Institute Australia, Nedlands, Western Australia, Australia. AD - Division of Emergency Medicine, Anaesthesia and Pain Medicine, The University of Western Australia, Perth, Western Australia, Australia. LA - eng GR - Stan Perron Charitable Foundation/ GR - 2009322/National Health and Medical Research Council (NHMRC), Australia/ GR - Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ PT - Journal Article PT - Review DEP - 20261029 PL - France TA - Paediatr Anaesth JT - Paediatric anaesthesia JID - 9206575 SB - IM OTO - NOTNLM OT - anxiety OT - fidget toys OT - neurodivergent OT - pediatric OT - sensory toy EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:53 PHST- 2026/09/21 00:00 [revised] PHST- 2026/08/07 00:00 [received] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:53 [entrez] AID - 10.1002/pan.70327 [doi] PST - aheadofprint SO - Paediatr Anaesth. 2026 Oct 29. doi: 10.1002/pan.70327. PMID- 42954774 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1755-5949 (Electronic) IS - 1755-5930 (Linking) VI - 32 IP - 11 DP - 2026 Nov TI - Plasma Lipidomic Features of Adolescent Major Depressive Disorder and Lipid Changes After Fluoxetine Treatment. LID - e71202 [pii] LID - 10.1002/cns.71202 [doi] AB - BACKGROUND: Major depressive disorder (MDD) in adolescents is a major public health concern, yet objective biological markers and treatment-related metabolic changes remain insufficiently understood. This study aimed to characterize plasma lipidomic alterations in adolescent MDD and explore lipid changes associated with fluoxetine treatment. METHODS: This study enrolled 75 adolescents with MDD and 75 matched healthy controls (HCs). Among the adolescents with MDD, 44 had paired baseline and week-8 plasma samples available and were included in the longitudinal analysis. Plasma lipidomic profiles were analyzed using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). RESULTS: A total of 865 lipid species were detected. Compared with HCs, adolescents with MDD showed distinct plasma lipidomic profiles, with 82 differentially abundant lipid species identified, primarily involving triglycerides, lysophospholipids, free fatty acids, membrane phospholipids, and sphingolipids. Among the machine-learning models, logistic regression showed the highest discriminative performance, with a pooled out-of-fold AUC of 0.85 (95% CI, 0.79-0.91). Longitudinal analysis identified 21 lipid species meeting the differential criteria following fluoxetine treatment, mainly involving triglycerides and bile acid-related metabolites. Integrative pathway and target analyses suggested that fluoxetine-associated lipid changes may involve neurotransmitter signaling, inflammatory lipid metabolism, PI3K-Akt signaling, and nuclear receptor-related pathways. CONCLUSION: This study characterizes alterations in plasma lipid metabolism in adolescent MDD and the lipidomic changes following fluoxetine treatment, providing new lipidomic evidence for candidate lipid signatures and potential treatment-associated biological pathways. TRIAL REGISTRATION: ClinicalTrials.gov, NCT05814640. Registered 20 February 2023, https://clinicaltrials.gov/study/NCT05814640. CI - (c) 2026 The Author(s). CNS Neuroscience & Therapeutics published by John Wiley & Sons Ltd. FAU - Liu, Chenxi AU - Liu C AUID- ORCID: 0009-0008-0332-6109 AD - Department of Psychiatry, Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Li, Yao AU - Li Y AUID- ORCID: 0009-0002-9173-7085 AD - Department of Psychiatry, Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Hou, Hanchen AU - Hou H AD - Department of Psychiatry, Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Guo, Jiamei AU - Guo J AD - Department of Psychiatry, Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Qin, Bingjie AU - Qin B AD - Department of Psychiatry, Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Xu, Xiaoxia AU - Xu X AUID- ORCID: 0009-0001-4827-8488 AD - Department of Psychiatry, Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - He, Yuqian AU - He Y AD - Department of Psychiatry, Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Lei, Tingting AU - Lei T AD - Department of Psychiatry, Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Chen, Fei AU - Chen F AUID- ORCID: 0000-0002-4522-3684 AD - Department of Psychiatry, Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Zhang, Lige AU - Zhang L AUID- ORCID: 0009-0002-8999-1674 AD - Department of Psychiatry, Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Liu, Quanbing AU - Liu Q AD - Department of Psychiatry, Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Gao, Li AU - Gao L AD - Department of Psychiatry, Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Shi, Jiaqi AU - Shi J AD - Department of Psychiatry, Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Xing, Haoran AU - Xing H AD - Department of Psychiatry, Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Teng, Teng AU - Teng T AD - Department of Psychiatry, Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Qiu, Tian AU - Qiu T AD - Department of Psychiatry, Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Zhou, Xinyu AU - Zhou X AUID- ORCID: 0000-0003-1908-0357 AD - Department of Psychiatry, Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. LA - eng SI - ClinicalTrials.gov/NCT05814640 GR - 2022ZD0212900/STI2030-Major Projects/ GR - 2024YFC2707800/National Key R&D Program of China/ GR - 82271565/the National Natural Science Foundation of China/ GR - 82301714/the National Natural Science Foundation of China/ GR - 82301713/the National Natural Science Foundation of China/ GR - CSTB2025YITP-QCRCX0084/New Chongqing Youth Innovation Talent Project/ GR - 2026QNKX006/Chongqing Medical Scientific Research Project: Joint Project of Chongqing Health Commission and Science and Technology Bureau/ PT - Journal Article PL - England TA - CNS Neurosci Ther JT - CNS neuroscience & therapeutics JID - 101473265 RN - 01K63SUP8D (Fluoxetine) RN - 0 (Lipids) RN - 0 (Antidepressive Agents, Second-Generation) RN - 0 (Selective Serotonin Reuptake Inhibitors) SB - IM MH - Humans MH - *Fluoxetine/therapeutic use/pharmacology MH - *Major Depressive Disorder/drug therapy/blood MH - *Lipidomics/methods MH - Adolescent MH - Female MH - Male MH - *Lipids/blood MH - Longitudinal Studies MH - *Antidepressive Agents, Second-Generation/therapeutic use MH - *Selective Serotonin Reuptake Inhibitors/therapeutic use MH - *Lipid Metabolism/drug effects MH - Chromatography, High Pressure Liquid OTO - NOTNLM OT - Major depressive disorder OT - adolescent OT - fluoxetine OT - lipidomics EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:52 PHST- 2026/09/18 00:00 [revised] PHST- 2026/07/20 00:00 [received] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:52 [entrez] AID - e71202 [pii] AID - 10.1002/cns.71202 [doi] PST - ppublish SO - CNS Neurosci Ther. 2026 Nov;32(11):e71202. doi: 10.1002/cns.71202. PMID- 42954773 OWN - NLM STAT- Publisher LR - 20261029 IS - 1521-4095 (Electronic) IS - 0935-9648 (Linking) DP - 2026 Oct 29 TI - Nb(5+)-Induced-CeO(2) Lattice Strain for Robust and Scalable Solar-Driven Ammonia Synthesis. LID - e75565 [pii] LID - 10.1002/adma.75565 [doi] AB - Ammonia (NH(3)) underpins fertilizer production and is emerging as a renewable-energy carrier. Solar-driven gas-phase N(2)/H(2) conversion requires catalysts that couple efficient light utilization with structural stability under H(2)-rich conditions. Here, Nb(5+) incorporation into CeO(2) contracts the lattice and induces compressive microstrain, suppressing excessive oxygen-sublattice reduction and preserving Ru dispersion. In situ NAP-XPS and computational results show that Nb-CeO(2) maintains an electron-enriched Ru working state under reaction conditions, which is further promoted by illumination. Accordingly, 5 wt% Ru/Nb-CeO(2) achieves an NH(3) synthesis rate of 7.4 mmol g(-1) h(-1) at 350 degrees C and 0.1 MPa and remains stable for 144 h. Kinetic analyses and in situ DRIFTS indicate that the electronically promoted Ru state alleviates N(2)-activation limitations and facilitates subsequent *NH(x) hydrogenation and NH(3) desorption. Scale-up synthesis affords a 3 wt% Ru/Nb-CeO(2)-L catalyst stable for 480 h and operable under outdoor concentrated sunlight. A screening-level analysis estimates a catalyst-and-feedstock cost of 286-322 USD t(NH3) (-1) under the lower-bound electricity scenario, 42%-51% below the selected Cs-Ru/MgO benchmark within the same framework. These results identify lattice strain as a promising strategy for simultaneously stabilizing the oxygen sublattice and electronically promoting Ru for efficient solar-driven ammonia synthesis. CI - (c) 2026 Wiley-VCH GmbH. FAU - Li, Can AU - Li C AUID- ORCID: 0009-0000-3380-9437 AD - Key Laboratory of Supramolecular Photochemistry and HKU-CAS Joint Laboratory on New Materials, New Cornerstone Science Laboratory, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, P. R. China. AD - School of Future Technology, University of Chinese Academy of Sciences, Beijing, P. R. China. FAU - Ru, Jin-Tao AU - Ru JT AUID- ORCID: 0009-0005-3965-6052 AD - Key Laboratory of Supramolecular Photochemistry and HKU-CAS Joint Laboratory on New Materials, New Cornerstone Science Laboratory, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, P. R. China. AD - School of Future Technology, University of Chinese Academy of Sciences, Beijing, P. R. China. FAU - Li, Jiaxuan AU - Li J AD - Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, P. R. China. FAU - Gao, Tengshijie AU - Gao T AUID- ORCID: 0009-0003-4232-7829 AD - Key Laboratory of Supramolecular Photochemistry and HKU-CAS Joint Laboratory on New Materials, New Cornerstone Science Laboratory, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, P. R. China. AD - School of Future Technology, University of Chinese Academy of Sciences, Beijing, P. R. China. FAU - Liu, Yi-Ming AU - Liu YM AD - Key Laboratory of Supramolecular Photochemistry and HKU-CAS Joint Laboratory on New Materials, New Cornerstone Science Laboratory, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, P. R. China. AD - School of Future Technology, University of Chinese Academy of Sciences, Beijing, P. R. China. FAU - Zhang, Rui-Min AU - Zhang RM AUID- ORCID: 0009-0003-1772-3467 AD - Key Laboratory of Supramolecular Photochemistry and HKU-CAS Joint Laboratory on New Materials, New Cornerstone Science Laboratory, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, P. R. China. AD - School of Future Technology, University of Chinese Academy of Sciences, Beijing, P. R. China. FAU - Liu, Hai-Xia AU - Liu HX AD - Key Laboratory of Supramolecular Photochemistry and HKU-CAS Joint Laboratory on New Materials, New Cornerstone Science Laboratory, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, P. R. China. AD - School of Future Technology, University of Chinese Academy of Sciences, Beijing, P. R. China. FAU - Wang, Xiao-Fang AU - Wang XF AD - Key Laboratory of Supramolecular Photochemistry and HKU-CAS Joint Laboratory on New Materials, New Cornerstone Science Laboratory, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, P. R. China. AD - School of Future Technology, University of Chinese Academy of Sciences, Beijing, P. R. China. FAU - Chen, Xiaotong AU - Chen X AD - Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, P. R. China. FAU - Su, Kaiyi AU - Su K AUID- ORCID: 0000-0003-1995-4990 AD - Key Laboratory of Supramolecular Photochemistry and HKU-CAS Joint Laboratory on New Materials, New Cornerstone Science Laboratory, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, P. R. China. AD - School of Future Technology, University of Chinese Academy of Sciences, Beijing, P. R. China. FAU - Li, Zhi-Jun AU - Li ZJ AUID- ORCID: 0000-0002-2897-0450 AD - Key Laboratory of Supramolecular Photochemistry and HKU-CAS Joint Laboratory on New Materials, New Cornerstone Science Laboratory, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, P. R. China. AD - School of Future Technology, University of Chinese Academy of Sciences, Beijing, P. R. China. FAU - Ye, Chen AU - Ye C AUID- ORCID: 0000-0003-2952-6586 AD - Key Laboratory of Supramolecular Photochemistry and HKU-CAS Joint Laboratory on New Materials, New Cornerstone Science Laboratory, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, P. R. China. AD - School of Future Technology, University of Chinese Academy of Sciences, Beijing, P. R. China. FAU - Tung, Chen-Ho AU - Tung CH AD - Key Laboratory of Supramolecular Photochemistry and HKU-CAS Joint Laboratory on New Materials, New Cornerstone Science Laboratory, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, P. R. China. AD - School of Future Technology, University of Chinese Academy of Sciences, Beijing, P. R. China. FAU - Wu, Li-Zhu AU - Wu LZ AUID- ORCID: 0000-0002-5561-9922 AD - Key Laboratory of Supramolecular Photochemistry and HKU-CAS Joint Laboratory on New Materials, New Cornerstone Science Laboratory, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, P. R. China. AD - School of Future Technology, University of Chinese Academy of Sciences, Beijing, P. R. China. LA - eng GR - 2024YFA1509700/National Key Research and Development Program of China/ GR - 2022YFA1503200/National Key Research and Development Program of China/ GR - 2022YFA1502900/National Key Research and Development Program of China/ GR - 22688202/National Natural Science Foundation of China/ GR - 92356309/National Natural Science Foundation of China/ GR - 22403101/National Natural Science Foundation of China/ GR - 22588101/National Natural Science Foundation of China/ GR - 22193013/National Natural Science Foundation of China/ GR - YSBR-142/CAS Project for Young Scientists in Basic Research/ GR - XDA0540000/Strategic Priority Research Program of the Chinese Academy of Science/ GR - XDB0960100/Strategic Priority Research Program of the Chinese Academy of Science/ GR - New Cornerstone Science Foundation/ PT - Journal Article DEP - 20261029 PL - Germany TA - Adv Mater JT - Advanced materials (Deerfield Beach, Fla.) JID - 9885358 SB - IM OTO - NOTNLM OT - Nb5+-incorporated CeO2 OT - ammonia synthesis OT - lattice strain OT - solar-driven catalysis EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:52 PHST- 2026/10/05 00:00 [revised] PHST- 2026/08/10 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:52 [entrez] AID - e75565 [pii] AID - 10.1002/adma.75565 [doi] PST - aheadofprint SO - Adv Mater. 2026 Oct 29:e75565. doi: 10.1002/adma.75565. PMID- 42954772 OWN - NLM STAT- Publisher LR - 20261029 IS - 1536-3732 (Electronic) IS - 1049-2275 (Linking) DP - 2026 Oct 28 TI - Complex Vertebral Artery Variation at the Craniovertebral Junction With V3 Segment Fenestration and External Carotid Artery-Derived Collateral Reconstruction. LID - 10.1097/SCS.0000000000013544 [doi] AB - BACKGROUND: The vertebral artery (VA) develops through a complex embryological process, and various anatomic variations may be encountered in adults. Vertebral artery fenestration (VAF) is a rare congenital vascular anomaly that may mimic vertebral artery dissection on imaging and requires careful differentiation based on vascular morphology and imaging findings. The coexistence of vertebral artery origin nonopacification, fenestration, and external carotid artery-derived collateral circulation is uncommon. CASE PRESENTATION: A 45-year-old man with no history of cerebrovascular disease or conventional vascular risk factors underwent magnetic resonance angiography (MRA) during a routine health examination. MRA revealed multiple cerebrovascular abnormalities, including bilateral vertebral artery stenosis, terminal basilar artery fenestration, persistent fetal-type right posterior cerebral artery, distal stenosis of the bilateral posterior cerebral arteries, stenosis at the bilateral anterior cerebral artery A1/A2 junctions, and cerebral atherosclerotic changes. The patient was asymptomatic, without neurological manifestations such as headache, dizziness, nausea, vomiting, diplopia, rotational vertigo, choking during drinking, dysphagia, or bowel and bladder dysfunction. Whole-brain digital subtraction angiography (DSA) was subsequently performed to further characterize the vascular anatomy.Digital subtraction angiography demonstrated nonopacification of the origin of the right vertebral artery, suggesting proximal occlusion. Selective right external carotid artery angiography revealed collateral vessels originating from branches of the deep cervical artery, costocervical trunk, and occipital artery. These vessels provided collateral supply to the V3 segment of the right vertebral artery through muscular anastomoses, resulting in reconstitution of the distal vertebral artery and visualization of the posterior circulation. CONCLUSION: This case demonstrates a rare pattern of complex vertebral artery anatomic variation. DSA not only identified the location of vertebral artery occlusion but also demonstrated the origin of abnormal collateral circulation and the pattern of vascular reconstruction, providing important diagnostic information for complex vertebral artery variations. CI - Copyright (c) 2026 by Mutaz B. Habal, MD. FAU - Zhu, Kefeng AU - Zhu K AD - Department of Thyroid and Breast Surgery, Affiliated Hospital of Shandong Second Medical University, Weifang, Shandong, China. FAU - Wang, Le AU - Wang L FAU - Li, Peirui AU - Li P AUID- ORCID: 0009-0009-5874-8015 LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Craniofac Surg JT - The Journal of craniofacial surgery JID - 9010410 SB - IM OTO - NOTNLM OT - Collateral circulation OT - V3 segment OT - digital subtraction angiography OT - fenestration OT - vascular variation OT - vertebral artery COIS- The authors report no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:43 PHST- 2026/09/20 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:43 [entrez] AID - 00001665-990000000-04759 [pii] AID - 10.1097/SCS.0000000000013544 [doi] PST - aheadofprint SO - J Craniofac Surg. 2026 Oct 28. doi: 10.1097/SCS.0000000000013544. PMID- 42954762 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1089-7623 (Electronic) IS - 0034-6748 (Linking) VI - 97 IP - 10 DP - 2026 Oct 1 TI - Upgrade of the RADCAM diagnostic for the TCV tokamak with tangential bolometer sightlines. LID - 103558 [pii] LID - 10.1063/5.0350441 [doi] AB - The plasma radiated power is a crucial component of transport and power balance analysis in modern fusion devices. In the Tokamak a Configuration Variable (TCV), radiated power inferences are currently provided by the RADCAM diagnostic. As part of a recent upgrade, two new bolometer arrays are being added to the system to improve both toroidal coverage and tomographic accuracy. This work details the principal elements of their preliminary design, the selection of views, and the proof of concept. The desired viewing geometry was modeled using ray tracing optimized to improve reconstructions. Synthetic emissivity distributions were used to assess the expected bolometer response and the inversion accuracy of the upgraded system. The technical design of the new bolometers is ongoing. Installation and exploitation is expected to take place by the end of 2026. CI - (c) 2026 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). FAU - Partesotti, G AU - Partesotti G AUID- ORCID: 0009-0008-9465-7885 AD - Ecole Polytechnique Federale de Lausanne (EPFL), Swiss Plasma Center (SPC), Lausanne, Switzerland. FAU - Sheikh, U AU - Sheikh U AUID- ORCID: 0000-0001-6207-2489 AD - Ecole Polytechnique Federale de Lausanne (EPFL), Swiss Plasma Center (SPC), Lausanne, Switzerland. FAU - Crisinel, F AU - Crisinel F AUID- ORCID: 0009-0003-4737-1327 AD - Ecole Polytechnique Federale de Lausanne (EPFL), Swiss Plasma Center (SPC), Lausanne, Switzerland. FAU - Hamm, D AU - Hamm D AUID- ORCID: 0009-0007-0783-5675 AD - Ecole Polytechnique Federale de Lausanne (EPFL), Swiss Plasma Center (SPC), Lausanne, Switzerland. FAU - Simons, L AU - Simons L AUID- ORCID: 0000-0002-5838-5076 AD - Ecole Polytechnique Federale de Lausanne (EPFL), Swiss Plasma Center (SPC), Lausanne, Switzerland. CN - TCV Team LA - eng PT - Journal Article PL - United States TA - Rev Sci Instrum JT - The Review of scientific instruments JID - 0405571 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:43 PHST- 2026/06/30 00:00 [received] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:43 [entrez] AID - 3407514 [pii] AID - 10.1063/5.0350441 [doi] PST - ppublish SO - Rev Sci Instrum. 2026 Oct 1;97(10):103558. doi: 10.1063/5.0350441. PMID- 42954753 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-3479 (Electronic) IS - 0020-7292 (Linking) DP - 2026 Oct 29 TI - Association of fetal epicardial fat thickness with adverse perinatal outcomes and assessment of fetal cardiac function in fetal growth restriction. LID - 10.1002/ijgo.71481 [doi] AB - OBJECTIVE: This study investigates the association between fetal epicardial fat thickness (EFT) and adverse perinatal outcomes in pregnancies complicated by fetal growth restriction (FGR), and assesses changes in fetal cardiac function. MATERIALS AND METHODS: This single-center, prospective observational study was conducted at a tertiary referral hospital. A total of 120 pregnancies between 28 and 37 weeks of gestation were enrolled: 40 with FGR and 80 healthy controls. EFT, fetal cardiac function parameters, and perinatal outcomes were compared between the FGR and control groups, and the same parameters were compared between the early-onset and late-onset FGR subgroups. Receiver operating characteristic analysis was used to evaluate the ability of EFT to discriminate composite adverse perinatal outcome (CAPO). RESULTS: Epicardial fat thickness was significantly lower in the FGR group than in controls (P < 0.001). Among fetal cardiac function parameters, the modified myocardial performance index (Mod-MPI) and isovolumetric relaxation time were significantly higher in the FGR group (P = 0.002 and P = 0.001, respectively), whereas ejection time was significantly shorter (P = 0.013). EFT was significantly lower in early-onset than in late-onset FGR (P < 0.001). Mod-MPI and isovolumetric contraction time were significantly higher in early-onset FGR (P < 0.05 for both). The rate of CAPO was higher in the FGR group (P < 0.001). CONCLUSION: In this cohort, reduced EFT was associated with FGR and with impaired fetal cardiac function. EFT is a simple, non-invasive parameter that might help identify FGR pregnancies at increased risk of adverse perinatal outcomes; however, larger multicenter studies are needed to confirm these findings and to validate the proposed cut-off value. CI - (c) 2026 International Federation of Gynecology and Obstetrics. FAU - Ozturk Agaoglu, Merve AU - Ozturk Agaoglu M AUID- ORCID: 0000-0002-1283-4032 AD - Department of Perinatology, Faculty of Medicine, Ankara Yildirim Beyazit University, Ankara, Turkey. AD - Department of Perinatology, Ankara Bilkent City Hospital, Ankara, Turkey. FAU - Bozkurt Ozdal, Burcu AU - Bozkurt Ozdal B AD - Department of Perinatology, Ankara Bilkent City Hospital, Ankara, Turkey. FAU - Reyhan Toptas, Gulnihal AU - Reyhan Toptas G AD - Department of Perinatology, Ankara Bilkent City Hospital, Ankara, Turkey. FAU - Ozden Tokalioglu, Eda AU - Ozden Tokalioglu E AD - Department of Perinatology, Ankara Bilkent City Hospital, Ankara, Turkey. FAU - Agaoglu, Zahid AU - Agaoglu Z AD - Department of Perinatology, Ankara Bilkent City Hospital, Ankara, Turkey. FAU - Sahin, Dilek AU - Sahin D AD - Department of Perinatology, Ankara Bilkent City Hospital, Ankara, Turkey. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Int J Gynaecol Obstet JT - International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics JID - 0210174 SB - IM OTO - NOTNLM OT - adverse perinatal outcome OT - epicardial fat thickness OT - fetal cardiac function OT - fetal growth restriction OT - modified myocardial performance index EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:43 PHST- 2026/10/05 00:00 [revised] PHST- 2026/08/01 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:43 [entrez] AID - 10.1002/ijgo.71481 [doi] PST - aheadofprint SO - Int J Gynaecol Obstet. 2026 Oct 29. doi: 10.1002/ijgo.71481. PMID- 42954737 OWN - NLM STAT- Publisher LR - 20261029 IS - 1098-5336 (Electronic) IS - 0099-2240 (Linking) DP - 2026 Oct 29 TI - A temperate phage acts as a symbiotic fitness enhancer for UPEC but surrenders to combination therapy through lysogeny suppression. PG - e0162126 LID - 10.1128/aem.01621-26 [doi] AB - Temperate phages are increasingly recognized as key drivers of bacterial evolution. Here, we isolated a myoviral prophage, vB_EcoM_P899, from a clinical uropathogenic Escherichia coli (UPEC) isolate and characterized how lysogenization shapes host biology. Lysogenization significantly enhanced biofilm formation, swarming motility, oxidative stress resistance, and adhesion to macrophages (up to 100-fold) without altering antibiotic susceptibility or overall 72-h lethality in the Galleria mellonella larval infection model. Mechanistically, this is associated with upregulation of the fimbrial gene FimA and enhanced inducibility of the oxidative stress response genes KatE and OxyR under oxidative stress. Crucially, combining P899 with ciprofloxacin or kanamycin produced synergistic killing and suppressed new lysogen formation in an antibiotic-class-dependent manner. In contrast, ceftazidime, while synergistic, failed to prevent lysogeny. Our findings demonstrate that while prophage vB_EcoM_P899 promotes bacterial niche adaptation, its lysogenization frequency is significantly reduced under specific antibiotic pressure, facilitating clearance of the challenged UPEC population. This work provides a strong rationale for optimizing phage-antibiotic combination therapies to treat drug-resistant infections by simultaneously enhancing bacterial killing and limiting lysogenic survivors. IMPORTANCE: This study reveals that lysogenization by a temperate phage can enhance the niche adaptation of uropathogenic Escherichia coli without increasing its virulence in the Galleria mellonella larval model or altering antibiotic resistance. Crucially, we demonstrate that combining this phage with select antibiotics (e.g., kanamycin and ciprofloxacin) not only kills the bacteria effectively but also actively suppresses the formation of new lysogens in an antibiotic-class-dependent manner. These findings uncover a novel role for temperate phages as symbiotic regulators within the host and provide direct experimental evidence for a phage-antibiotic combination strategy. This approach holds promise for combating chronic, recurrent urinary tract infections and offers a clinically viable pathway to address antibiotic tolerance in chronic, recurrent urinary tract infections. FAU - Yan, Bo AU - Yan B AUID- ORCID: 0009-0002-2079-4621 AD - Health Science Center, Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 FAU - Yan, Can AU - Yan C AD - Health Science Center, Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 FAU - Li, Qingwen AU - Li Q AD - Health Science Center, Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 FAU - Cai, Siyi AU - Cai S AD - Health Science Center, Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 FAU - Zhu, Haotian AU - Zhu H AD - Health Science Center, Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 FAU - Song, Mingxia AU - Song M AD - Health Science Center, Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 AD - Jiangxi Provincial Key Laboratory of Organ Development and Epigenetics, Clinical Medical Research Center, Affiliated Hospital of Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 FAU - Deng, Xianqing AU - Deng X AD - Health Science Center, Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 AD - Jiangxi Provincial Key Laboratory of Organ Development and Epigenetics, Clinical Medical Research Center, Affiliated Hospital of Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 FAU - Huang, Xiaoliu AU - Huang X AD - Health Science Center, Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 AD - Jiangxi Provincial Key Laboratory of Organ Development and Epigenetics, Clinical Medical Research Center, Affiliated Hospital of Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 FAU - Guo, Wenqiang AU - Guo W AD - Health Science Center, Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 AD - Jiangxi Provincial Key Laboratory of Organ Development and Epigenetics, Clinical Medical Research Center, Affiliated Hospital of Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 FAU - Qin, Kunhao AU - Qin K AUID- ORCID: 0000-0002-3740-4661 AD - Health Science Center, Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 AD - Jiangxi Provincial Key Laboratory of Organ Development and Epigenetics, Clinical Medical Research Center, Affiliated Hospital of Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 FAU - Fu, Qiang AU - Fu Q AUID- ORCID: 0000-0002-7192-9306 AD - Health Science Center, Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 AD - Jiangxi Provincial Key Laboratory of Organ Development and Epigenetics, Clinical Medical Research Center, Affiliated Hospital of Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 FAU - Ding, Yafang AU - Ding Y AUID- ORCID: 0009-0001-6907-2251 AD - Health Science Center, Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 AD - Jiangxi Provincial Key Laboratory of Organ Development and Epigenetics, Clinical Medical Research Center, Affiliated Hospital of Jinggangshan University, Ji'an, China. RINGGOLD: 105809. ROR: https://ror.org/04exd0a76 LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Appl Environ Microbiol JT - Applied and environmental microbiology JID - 7605801 SB - IM OTO - NOTNLM OT - UPEC OT - lysogenic conversion OT - lysogenic phage OT - oxidative stress OT - phage-antibiotic combination therapy EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:33 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:33 [entrez] AID - 10.1128/aem.01621-26 [doi] PST - aheadofprint SO - Appl Environ Microbiol. 2026 Oct 29:e0162126. doi: 10.1128/aem.01621-26. PMID- 42954736 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2164-2591 (Electronic) IS - 2164-2591 (Linking) VI - 15 IP - 10 DP - 2026 Oct 5 TI - Repeatability and Cross-Domain Agreement of Pyramidal-Sensor Ocular Wavefront Measurements in Myopic Eyes: A Registry Validation of OSIRIS. PG - 49 LID - 10.1167/tvst.15.10.49 [doi] AB - PURPOSE: The purpose of this study was to quantify within-session repeatability of ocular wavefront metrics from a pyramidal wavefront-sensor aberrometer (OSIRIS; CSO), to define a pupil-support and zero-code gate for 6-mm analysis, and to assess agreement between OSIRIS whole-eye and MS-39 total-corneal wavefront. METHODS: Retrospective single-center registry analysis of myopic and myopic astigmatic eyes. Within-subject standard deviation (SD), repeatability coefficient (RC = 2.77 x SD), and intraclass correlation coefficient ICC (1.1) were computed across within-session scan clusters with cluster-bootstrap confidence intervals (CIs). Agreement used Bland-Altman limits and Lin concordance correlation coefficient (CCC); and astigmatism used Thibos power vectors. RESULTS: Across 10,416 sessions from 2533 eyes, single-scan repeatability was excellent: 4-mm higher-order aberration (HOA) root mean square (RMS), ICC = 0.987 (RC = 0.033 microm); pupil-supported 6-mm HOA RMS ICC = 0.992 (RC = 0.050 microm); and 6-mm spherical aberration ICC = 0.997. Preoperative eyes were the noisier stratum (6-mm HOA RC = 0.065 vs. 0.039 postoperatively). Diameters below 6 mm gave entirely zero-coded 6-mm exports in 96.8% of sessions. Omitting scan-selection worsened 6-mm HOA RC to 0.257 microm. Whole-eye and total-corneal values correlated but were not interchangeable (6-mm HOA RMS CCC = 0.623). Objective and manifest refraction were consistent (4-mm sphere CCC = 0.965), a rounding-limited consistency check. CONCLUSIONS: In myopic eyes, OSIRIS shows excellent within-session repeatability under the described acquisition and quality-control (QC) workflow. Six-millimeter analysis requires pupil-diameter and zero-code gating. Whole-eye and total-corneal wavefront are not interchangeable. TRANSLATIONAL RELEVANCE: Within-session precision measured in routine high-volume practice, with a pupil-diameter and zero-code quality rule, defines the smallest same-session change distinguishable from measurement variability, informing wavefront-guided planning, lens selection, and post-refractive follow-up. FAU - Gabric, Ivan AU - Gabric I AD - Eye Clinic Svjetlost, Zagreb, Croatia. AD - University of Zagreb School of Medicine, Zagreb, Croatia. FAU - Modric, Ivan Samuel AU - Modric IS AD - Eye Clinic Svjetlost, Zagreb, Croatia. FAU - Grulovic, Filip AU - Grulovic F AD - Eye Clinic Svjetlost, Zagreb, Croatia. LA - eng PT - Journal Article PT - Validation Study PL - United States TA - Transl Vis Sci Technol JT - Translational vision science & technology JID - 101595919 SB - IM MH - Humans MH - Retrospective Studies MH - Reproducibility of Results MH - *Aberrometry/instrumentation/methods MH - *Registries MH - *Myopia/physiopathology/diagnosis MH - Female MH - Male MH - Middle Aged MH - *Refraction, Ocular/physiology MH - Astigmatism/physiopathology/diagnosis MH - Adult MH - *Corneal Wavefront Aberration/physiopathology/diagnosis MH - Cornea/physiopathology MH - Visual Acuity/physiology EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 17:36 CRDT- 2026/10/29 09:33 PHST- 2026/10/29 17:36 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:33 [entrez] AID - 2818787 [pii] AID - 10.1167/tvst.15.10.49 [doi] PST - ppublish SO - Transl Vis Sci Technol. 2026 Oct 5;15(10):49. doi: 10.1167/tvst.15.10.49. PMID- 42954727 OWN - NLM STAT- Publisher LR - 20261029 IS - 1724-6075 (Electronic) IS - 0391-5603 (Linking) DP - 2026 Oct 29 TI - Zone-specific pathological correlates of PI-RADS 4-5 lesions and the role of multiparametric MRI lesion size in predicting pathological upstaging: A series of radical prostatectomies. PG - 3915603261499067 LID - 10.1177/03915603261499067 [doi] AB - PURPOSE: Using radical retropubic prostatectomy (RRP) specimens as the reference standard, we assessed whether PI-RADS 4-5 lesions in the transitional zone (TZ) and peripheral zone (PZ) differ in their association with clinically significant prostate cancer (csPCa), whether the composition of non-csPCa lesions differs by zone, and whether multiparametric MRI (mpMRI) lesion size predicts pathological upstaging. MATERIALS AND METHODS: Preoperative mpMRI data from 55 patients (108 lesions) undergoing RRP were retrospectively analyzed. A single experienced radiologist scored lesions using PI-RADS v2.1; each was correlated with the specimen by anatomical compartment. PI-RADS 4-5 lesions were classified as (A) csPCa (ISUP Grade Group ⩾ 2), (B) ISUP GG1, or (C) no neoplastic correlate within the corresponding compartment. Upstaging was defined as clinical T2a/T2b to surgical ⩾T2c, or clinical T2c to surgical ⩾T3a. RESULTS: csPCa was identified in 25.9% (7/27) of TZ PI-RADS 4-5 lesions versus 70.0% (35/50) in the PZ (p < 0.001). Maximum lesion diameter was larger in patients with upstaging (18.7 +/- 7.6 vs 11.8 +/- 2.8 mm; p = 0.0003); the AUC was 0.808 (95% CI 0.694-0.922) and the Youden-selected cut-off was ⩾13 mm (sensitivity 87.9%, specificity 61.9%, OR = 11.78). After adjustment for age and PSA, lesion size remained the only significant predictor (aOR = 1.294 per mm, p = 0.003), and the association persisted when the endpoint was restricted to extraprostatic extension (per 10 mm: OR = 4.96, 95% CI 1.90-16.92). Among non-csPCa lesions, 11 of 20 in the TZ had no neoplastic correlate versus 1 of 15 in the PZ (p = 0.004). CONCLUSION: Larger mpMRI lesion size was associated with pathological upstaging, an association that persisted after adjustment and under a stricter endpoint; the ⩾13 mm threshold is exploratory pending external validation. Most TZ PI-RADS 4-5 lesions were non-csPCa, and lesions lacking a neoplastic correlate were more frequent in the TZ, although this comparison rested on a single such lesion in the PZ and is hypothesis-generating. FAU - Isler, Bilgi AU - Isler B AUID- ORCID: 0000-0003-1243-9072 AD - Department of Urology, Konya City Hospital, University of Health Sciences, Konya, Turkey. FAU - Bayraktar, Ahmet Murat AU - Bayraktar AM AUID- ORCID: 0000-0002-4913-8698 AD - Department of Urology, Konya City Hospital, University of Health Sciences, Konya, Turkey. FAU - Odabas, Oner AU - Odabas O AD - Department of Urology, Ankara Bilkent City Hospital, University of Health Sciences, Konya, Turkey. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Urologia JT - Urologia JID - 0417372 SB - IM OTO - NOTNLM OT - PI-RADS OT - multiparametric magnetic resonance imaging OT - pathological upstaging OT - prostate cancer OT - radical prostatectomy OT - transitional zone EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:33 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:33 [entrez] AID - 10.1177/03915603261499067 [doi] PST - aheadofprint SO - Urologia. 2026 Oct 29:3915603261499067. doi: 10.1177/03915603261499067. PMID- 42954725 OWN - NLM STAT- Publisher LR - 20261029 IS - 1724-6075 (Electronic) IS - 0391-5603 (Linking) DP - 2026 Oct 29 TI - Real-world diagnostic performance of pre-TURBT multiparametric MRI using VI-RADS for detecting muscle-invasive bladder cancer: A retrospective single-center study. PG - 3915603261499101 LID - 10.1177/03915603261499101 [doi] AB - INTRODUCTION: VI-RADS (Vesical Imaging-Reporting and Data System) is a standardized scoring system developed to assess the likelihood of muscle-invasive bladder cancer (MIBC) using multiparametric magnetic resonance imaging (mpMRI). Our aim was to report our 3-year experience in using mpMRI to differentiate MIBC from non-muscle invasive bladder cancer (NMIBC). All radiological reports were generated using the VI-RADS score. MATERIALS AND METHODS: Patients with finding of ⩾15 mm suspicious bladder cancer at Ultrasound, CT scan or flexible cystoscopy underwent mpMRI before transurethral resection of bladder tumor (TURBT). We used a 5-point VI-RADS score, derived using T2-weighted MRI, diffusion-weighted imaging, and dynamic contrast enhancement. All examinations were performed on a 1.5 T scanner. In these 3 years images were analyzed by dedicated genitourinary radiologists and all TURBT were performed by expert urologists adhering to the European guidelines' recommendations in order to strive for high-quality resections. Accuracy was determined using histopathology as the reference standard. RESULTS: A total of 90 patients were analyzed, both primary and recurrent. Overall accuracy of the complete mpMRI protocol was 87% in differentiating NMIBC from MIBC. Better accuracy was achieved for ⩾25 mm (92%) and lateral wall sided (91%) lesions, while a lower one was recorded for dome tumors (86%). Hematuria, cytology, class of risks, focality and patients' features did not impact on results. No differences were recorded for histological variants. CONCLUSIONS: mpMRI with VI-RADS showed good diagnostic performance in differentiating muscle-invasive from non-muscle-invasive bladder cancer in a real-world clinical setting. Further prospective studies with larger cohorts are warranted to confirm these findings. FAU - Ola, Luciano AU - Ola L AUID- ORCID: 0009-0009-8844-4311 AD - Division of Urology, Santa Croce e Carle Hospital, Cuneo, Italy. FAU - Busacca, Giovanni AU - Busacca G AUID- ORCID: 0009-0002-9029-2280 AD - Division of Urology, Santa Croce e Carle Hospital, Cuneo, Italy. FAU - Dalmasso, Ettore AU - Dalmasso E AD - Division of Urology, Santa Croce e Carle Hospital, Cuneo, Italy. FAU - Giordano, Andrea AU - Giordano A AD - Division of Urology, Mauriziano Hospital, Turin, Italy. FAU - Pisano, Chiara AU - Pisano C AD - Division of Oncology, Santa Croce e Carle Hospital, Cuneo, Italy. FAU - Meziere, Juliette AU - Meziere J AD - Division of Urology, Santa Croce e Carle Hospital, Cuneo, Italy. FAU - Ornaghi, Paola Irene AU - Ornaghi PI AD - Division of Urology, Santa Croce e Carle Hospital, Cuneo, Italy. FAU - Poletti, Filippo AU - Poletti F AD - Division of Urology, Santa Croce e Carle Hospital, Cuneo, Italy. FAU - Morra, Ivano AU - Morra I AD - Division of Urology, Santa Croce e Carle Hospital, Cuneo, Italy. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Urologia JT - Urologia JID - 0417372 SB - IM OTO - NOTNLM OT - VI-RADS score OT - bladder tumor staging OT - magnetic resonance imaging OT - multiparametric OT - muscle-invasive bladder cancer OT - urinary bladder neoplasms EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:33 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:33 [entrez] AID - 10.1177/03915603261499101 [doi] PST - aheadofprint SO - Urologia. 2026 Oct 29:3915603261499101. doi: 10.1177/03915603261499101. PMID- 42954722 OWN - NLM STAT- Publisher LR - 20261029 IS - 1540-0514 (Electronic) IS - 1073-2322 (Linking) DP - 2026 Oct 29 TI - Consensus-derived clinical sepsis profiles and mortality across six international cohorts. LID - 10.1097/SHK.0000000000002954 [doi] AB - BACKGROUND: Clinical phenotyping may help characterize sepsis heterogeneity, but the applicability of derived profiles across healthcare settings remains uncertain. We evaluated consensus-derived clinical profiles and their associations with mortality across six international cohorts. METHODS: We analyzed 62,458 adults from six critical care databases in four countries. In the MIMIC-IV discovery cohort (n = 28,733), six clustering algorithms were applied to 24 routinely available clinical variables to derive consensus profiles. The profiles were assigned in five external cohorts using fixed discovery-cohort templates. We examined clinical characteristics, mortality associations, and exploratory treatment-outcome associations. RESULTS: Three profiles were selected based on the Calinski-Harabasz peak and clinical interpretability. Profiles 1, 2, and 3 comprised 32.6%, 20.0%, and 47.4% of the discovery cohort, with in-hospital mortality of 30.3%, 13.8%, and 9.3%, respectively. Profile 1 showed greater multiorgan dysfunction, Profile 2 relatively preserved hemodynamics, and Profile 3 greater ventilatory support with less extrapulmonary dysfunction. Profile 1 had the highest mortality in all five external cohorts, with a decreasing mortality gradient across profiles in most cohorts. After adjustment for age and SOFA score, Profile 3 was associated with lower 1-year mortality than Profile 1 (hazard ratio 0.62, 95% CI 0.57-0.67). Treatment-outcome associations were exploratory. CONCLUSIONS: Consensus-derived profiles characterized clinically interpretable patterns of sepsis heterogeneity with mortality differences across the evaluated cohorts. These findings support further evaluation of clinical profiling for risk stratification and phenotype-informed research. CI - Copyright (c) 2026 by the Shock Society. FAU - Shen, Ying-Ying AU - Shen YY AD - The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou 310006, China. FAU - Yi, Ru-Qin AU - Yi RQ AD - The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou 310006, China. FAU - Chen, Wei AU - Chen W AD - The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou 310006, China. FAU - Li, Cheng-En AU - Li CE AD - The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou 310006, China. FAU - Zhang, Ding AU - Zhang D AD - The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou 310006, China. FAU - Miao, Sheng-Hui AU - Miao SH AD - The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou 310006, China. FAU - Zhang, Zhi-Rong AU - Zhang ZR AD - The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou 310006, China. FAU - Fan, Zhao-Kun AU - Fan ZK AD - The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou 310006, China. AD - The Second School of Clinical Medicine, Zhejiang Chinese Medical University, Hangzhou 310006, China. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Shock JT - Shock (Augusta, Ga.) JID - 9421564 SB - IM OTO - NOTNLM OT - clinical phenotyping OT - consensus clustering OT - external evaluation OT - mortality OT - organ dysfunction OT - risk stratification OT - sepsis EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:33 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:33 [entrez] AID - 00024382-990000000-00960 [pii] AID - 10.1097/SHK.0000000000002954 [doi] PST - aheadofprint SO - Shock. 2026 Oct 29. doi: 10.1097/SHK.0000000000002954. PMID- 42954693 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2772-963X (Electronic) IS - 2772-963X (Linking) VI - 5 IP - 10 Pt 2 DP - 2026 Oct TI - Norepinephrine Equivalent Dose for Mortality Risk Stratification in VA-ECMO Cardiogenic Shock. PG - 103208 LID - S2772-963X(26)00629-0 [pii] LID - 10.1016/j.jacadv.2026.103208 [doi] AB - BACKGROUND: Patients with cardiogenic shock (CS) complicated by vasoplegia requiring venoarterial extracorporeal membrane oxygenation (VA-ECMO) often need escalating vasopressor support and face high mortality. Norepinephrine equivalent dose (NEE) quantifies vasopressor burden, yet its prognostic value in VA-ECMO remains undefined. OBJECTIVES: We aimed to characterize the dose-response relationship between NEE and 30-day mortality, identify a clinically relevant NEE threshold, and determine factors associated with high vasopressor burden. METHODS: This bicentric retrospective study included 572 consecutive patients with CS requiring VA-ECMO. We collected vasopressor regimens, NEE trajectories, and hemodynamic parameters at predefined intervals. Restricted cubic splines assessed dose-response linearity. Maximally selected rank statistics with bootstrap validation identified optimal thresholds. Cox models estimated mortality risk. Multivariable logistic regression identified factors associated with high vasopressor burden. RESULTS: The median NEE at ECMO initiation was 0.30 (IQR: 0.09-0.72) mug.kg(-1).min(-1); 86.2% received norepinephrine. NEE showed a linear dose-response with mortality (HR: 1.06 per 0.1 mug.kg(-1).min(-1) increment; 95% CI: 1.04-1.08; P < 0.001). A threshold of 0.443 mug.kg(-1).min(-1) identified high vasopressor burden with elevated 30-day mortality (61.8% vs 42.6%; P < 0.001; adjusted HR: 1.42; 95% CI: 1.11-1.81; P = 0.005). Factors associated with high vasopressor burden included mechanical ventilation (OR: 2.96; 95% CI: 1.08-8.07), prior epinephrine use (OR: 1.87; 95% CI: 1.12-3.14), renal replacement therapy before VA-ECMO (OR: 1.76; 95% CI: 1.02-3.03), baseline lactate (OR: 1.05 per mmol/L; 95% CI: 1.01-1.09), and lower Survival After Veno-Arterial ECMO score (OR: 0.96 per point; 95% CI: 0.92-1.00). Left ventricular unloading was inversely associated (OR: 0.54; 95% CI: 0.34-0.87). CONCLUSIONS: In VA-ECMO-supported CS with vasoplegia, vasopressor burden shows a linear relationship with mortality. A NEE threshold of 0.443 mug.kg(-1).min(-1) may identify patients with elevated mortality risk. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Guinot, Pierre-Gregoire AU - Guinot PG AD - Department of Anesthesiology and Intensive Care, Dijon University Hospital, Dijon, France; University of Burgundy Europe, LNC UMR1231, Dijon, France; INSERM, LNC UMR1231, Dijon, France; FCS Bourgogne-Franche Comte, LipSTIC LabEx, Dijon, France. Electronic address: guinotpierregregoire@gmail.com. FAU - Nguyen, Maxime AU - Nguyen M AD - Department of Anesthesiology and Intensive Care, Dijon University Hospital, Dijon, France; University of Burgundy Europe, LNC UMR1231, Dijon, France; INSERM, LNC UMR1231, Dijon, France; FCS Bourgogne-Franche Comte, LipSTIC LabEx, Dijon, France. FAU - Abou-Arab, Osama AU - Abou-Arab O AD - Department of Anesthesiology and Intensive Care, Amiens University Hospital, Amiens, France. FAU - Mahjoub, Yazine AU - Mahjoub Y AD - Department of Anesthesiology and Intensive Care, Amiens University Hospital, Amiens, France. FAU - Bouhemad, Belaid AU - Bouhemad B AD - Department of Anesthesiology and Intensive Care, Dijon University Hospital, Dijon, France; University of Burgundy Europe, LNC UMR1231, Dijon, France; INSERM, LNC UMR1231, Dijon, France; FCS Bourgogne-Franche Comte, LipSTIC LabEx, Dijon, France. FAU - Beyls, Christophe AU - Beyls C AD - Department of Anesthesiology and Intensive Care, Amiens University Hospital, Amiens, France; UR UPJV 7518 SSPC (Simplification of Care of Complex Surgical Patients) Research Unit, University of Picardie Jules Verne, Amiens, France. LA - eng PT - Journal Article PL - United States TA - JACC Adv JT - JACC. Advances JID - 9918419284106676 OTO - NOTNLM OT - cardiogenic shock OT - extracorporeal membrane oxygenation OT - mortality OT - norepinephrine equivalent dose OT - risk stratification OT - vasopressor COIS- Funding support and author disclosures Drs Nguyen, Pierre-Gregoire, and Abou-Arab have received fees for lectures from Baxter. Dr Guinot has received fees for lectures from AOP, Medtronic, Edwards, and Vygon; and is a consultant for Abbott. All other authors have reported that they have no relationships relevant to the contents of this paper to disclose. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:28 PHST- 2026/04/17 00:00 [received] PHST- 2026/07/24 00:00 [revised] PHST- 2026/08/01 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:28 [entrez] AID - S2772-963X(26)00629-0 [pii] AID - 10.1016/j.jacadv.2026.103208 [doi] PST - ppublish SO - JACC Adv. 2026 Oct;5(10 Pt 2):103208. doi: 10.1016/j.jacadv.2026.103208. PMID- 42954676 OWN - NLM STAT- Publisher LR - 20261029 IS - 1532-5040 (Electronic) IS - 0959-3985 (Linking) DP - 2026 Oct 29 TI - The relationship between pain-related psychological factors and balance performance in individuals with knee osteoarthritis: A systematic review. PG - 1-15 LID - 10.1080/09593985.2026.2753664 [doi] AB - BACKGROUND AND PURPOSE: Balance impairments have been well documented in individuals with knee osteoarthritis (KOA). Despite the growing body of evidence addressing the clinical relevance of pain‑related psychological factors in individuals with KOA, the association between these factors and balance performance remains unclear. Therefore, this study aimed to systematically synthesize and critically appraise the available evidence regarding the associations between pain‑related psychological factors and balance performance in individuals with KOA. METHODS: Case-control, cross-sectional, or cohort study designs were included in this review study. PubMed, Scopus, Web of Science, Embase, Google Scholar, and ResearchGate were searched from their inception to 18 May 2026. The selected search terms were associated with pain-related psychological factors (e.g. pain self-efficacy (PSE), kinesiophobia, pain catastrophizing (PC), and fear-avoidance beliefs (FAB)), KOA, and balance performance. RESULTS: All 13 included studies exhibited a low risk of bias in the selection and outcome domains; regarding comparability, 4 studies demonstrated a low risk of bias, while 9 showed a high risk of bias according to the adapted Newcastle-Ottawa scale. The results revealed weak and non-significant correlations for kinesiophobia in the majority of the studies (62.5%). For PC, among three studies assessing the effects of PC on balance performance, two studies found a significant negative correlation between PC and balance performance. Regarding the PSE and FAB, evidence was limited to only two studies for each component with inconsistent results. CONCLUSION: PC may be associated with poorer balance performance in individuals with KOA. However, the available evidence remains limited and inconsistent. Findings regarding the other pain related psychological factors were inconsistent, potentially reflecting variations in the assessment tools used to measure painrelated psychological factors and balance performance across studies. Further studies are warranted to investigate the impacts of positive and negative pain related psychological factors on balance performance in individuals with KOA. FAU - Abolhasani, Mohammad Mehdi AU - Abolhasani MM AD - Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. AD - Rehabilitation Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. FAU - Mofateh, Razieh AU - Mofateh R AD - Rehabilitation Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. AD - Department of Physiotherapy, School of Rehabilitation Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. FAU - Hosseinizadeh, Fatemeh AU - Hosseinizadeh F AD - Rehabilitation Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - Physiother Theory Pract JT - Physiotherapy theory and practice JID - 9015520 SB - IM OTO - NOTNLM OT - Knee osteoarthritis OT - balance performance OT - chronic pain OT - psychological factor EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:25 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:25 [entrez] AID - 10.1080/09593985.2026.2753664 [doi] PST - aheadofprint SO - Physiother Theory Pract. 2026 Oct 29:1-15. doi: 10.1080/09593985.2026.2753664. PMID- 42954675 OWN - NLM STAT- Publisher LR - 20261029 IS - 1536-3732 (Electronic) IS - 1049-2275 (Linking) DP - 2026 Oct 29 TI - Autologous Tissue Transfer for Total Facial Reconstruction: Principles and the Chinese Experience. LID - 10.1097/SCS.0000000000013569 [doi] AB - Severe facial disfigurement remains a complex reconstructive challenge involving the facial skeleton, soft-tissue envelope, sensory and motor systems, and specialized facial features. Burn-related surface loss, high-energy trauma, and deep composite defects require distinct reconstructive strategies. Reconstruction aims to preserve and restore essential functions, reconstruct facial form, and support psychological recovery and self-confidence. Autologous facial reconstruction encompasses skin, mucosal, cartilage, bone, fat, fascial, and nerve grafts; local and regional flaps; free tissue transfer; and functional muscle transfer. Its principal immunologic advantage is the avoidance of alloimmune rejection, without the need for lifelong immunosuppression solely to preserve the transferred tissue. Nevertheless, autologous reconstruction is constrained by tissue matching, donor-site availability, recipient vascular status, and the capacity to restore dynamic facial function. Over recent decades, Chinese reconstructive teams have advanced extensive and total facial reconstruction through tissue expansion, flap prefabrication, prelamination, vascular supercharging, three-dimensional planning, and indocyanine green angiography. Long-term studies have reported sustained benefits in selected patients. This narrative review draws on targeted retrieval of PubMed-indexed publications and primary journal articles, supplemented by reference tracing. Searches combined terms related to autologous facial reconstruction, total face restoration, tissue expansion, flap prefabrication, prelamination, and reconstruction of individual facial features. Chinese experience was defined by the clinical setting and institutional affiliations reported in each publication, including international collaborations. The review synthesizes advances in autologous facial reconstruction, with particular emphasis on the total facial reconstruction series from Shanghai Ninth People's Hospital and complementary experience from other Chinese plastic and reconstructive surgery centers. These contributions are organized into 9 practice-based reconstructive principles: (1) functional restoration and preservation of facial tissue, (2) the Matching, Large, Thin principle and facial aesthetic unit matching, (3) tissue expansion and donor-site protection, (4) flap prefabrication and prelamination, (5) tissue perfusion and flap supercharging, (6) layered three-dimensional reconstruction, (7) staged functional reconstruction, (8) particular considerations for pediatric autologous reconstruction, and (9) psychosocial care and social reintegration. The Chinese experience supports early assessment of autologous options when essential functional goals can be achieved with acceptable donor-site morbidity. This approach has particular clinical and ethical relevance to children with burn-related or traumatic facial defects. Its potential application to war-related injuries requires careful consideration of defect composition, treatment phase, and continuity of care. Facial allotransplantation retains a distinct role when profound composite tissue loss exceeds the capabilities of autologous reconstruction. Further refinement of flap prefabrication, integration of digital planning, advances in regenerative medicine, and standardized outcome assessment may improve reconstructive predictability and durability. Longitudinal evaluation of functional, aesthetic, psychosocial, and donor-site outcomes is essential to establish the broader applicability of these approaches and strengthen the evidence guiding reconstruction of severe facial injuries. CI - Copyright (c) 2026 by Mutaz B. Habal, MD. FAU - Xu, Haisong AU - Xu H AD - Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Craniofac Surg JT - The Journal of craniofacial surgery JID - 9010410 SB - IM OTO - NOTNLM OT - Autologous facial reconstruction OT - craniofacial surgery OT - extensive facial injury OT - flap prefabrication OT - pediatric facial reconstruction OT - tissue expansion COIS- The author reports no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:25 PHST- 2026/10/14 00:00 [received] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:25 [entrez] AID - 00001665-990000000-04763 [pii] AID - 10.1097/SCS.0000000000013569 [doi] PST - aheadofprint SO - J Craniofac Surg. 2026 Oct 29. doi: 10.1097/SCS.0000000000013569. PMID- 42954667 OWN - NLM STAT- Publisher LR - 20261029 IS - 1542-6270 (Electronic) IS - 1060-0280 (Linking) DP - 2026 Oct 29 TI - Bictegravir/Lenacapavir: A 2-Drug Regimen for the Treatment of HIV-1 Infection. PG - 10600280261496876 LID - 10.1177/10600280261496876 [doi] AB - OBJECTIVE: To evaluate the efficacy and safety of bictegravir/lenacapavir for the treatment of HIV-1 infection. DATA SOURCES: A literature search was conducted in PubMed and Google Scholar (January 2016-August 2026) using the search terms bictegravir and lenacapavir. Other data sources included abstracts presented at recent conferences and the prescribing information. STUDY SELECTION AND DATA EXTRACTION: All English-language studies assessing the efficacy and safety of bictegravir/lenacapavir for the treatment of HIV-1 infection were considered for inclusion. DATA SYNTHESIS: Bictegravir is an established second-generation integrase strand transfer inhibitor (INSTI), whereas lenacapavir is a novel capsid inhibitor. Both antiretrovirals are highly active against HIV-1. Bictegravir/lenacapavir 75 mg/50 mg was shown to be safe and effective in two phase 3 clinical trials after switching from complex antiretroviral therapy (ART) or bictegravir/emtricitabine/tenofovir alafenamide. The Food and Drug Administration approved this combination for the treatment of HIV-1 infection in adults who are virologically suppressed on stable ART with no resistance to the individual components. It is available as a single-tablet regimen (STR) taken orally once daily.Relevance to Patient Care and Clinical Practice in Comparison With Existing Drugs:Bictegravir/lenacapvir is the first INSTI-capsid inhibitor 2-drug STR indicated for the treatment of HIV-1 infection. The combination could be useful for patients who want to simplify their regimen and/or those who cannot use a nucleos(t)ide or a non-nucleoside reverse transcriptase inhibitor. It is not active against hepatitis B virus coinfection. CONCLUSIONS: Bictegravir/lenacapvir is safe and efficacious as a 2-drug regimen for the treatment of adults with HIV-1 infection. FAU - Chahine, Elias B AU - Chahine EB AUID- ORCID: 0000-0003-1775-9497 AD - Department of Pharmacy Practice and Administration, Palm Beach Atlantic University Gregory School of Pharmacy, West Palm Beach, FL, USA. FAU - Faragon, John J AU - Faragon JJ AUID- ORCID: 0009-0006-5326-4773 AD - HIV Medicine, Department of Pharmacy, Albany Med Health System, NY, USA. FAU - Patel, Nimish AU - Patel N AUID- ORCID: 0000-0003-0921-549X AD - Department of Pharmacy Practice and Sciences, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, USA. FAU - Sherman, Elizabeth M AU - Sherman EM AUID- ORCID: 0000-0002-6559-0684 AD - Department of Pharmacy Practice, Nova Southeastern University Silverman College of Pharmacy, Fort Lauderdale, FL, USA. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - United States TA - Ann Pharmacother JT - The Annals of pharmacotherapy JID - 9203131 SB - IM OTO - NOTNLM OT - HIV/AIDS OT - antiretrovirals OT - clinical trials OT - drug efficacy EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:24 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:24 [entrez] AID - 10.1177/10600280261496876 [doi] PST - aheadofprint SO - Ann Pharmacother. 2026 Oct 29:10600280261496876. doi: 10.1177/10600280261496876. PMID- 42954662 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-3479 (Electronic) IS - 0020-7292 (Linking) DP - 2026 Oct 29 TI - Single-incision mini-sling (SIMS) under local anesthesia for stress urinary incontinence: A single-center prospective cohort study-12 months of follow-up. LID - 10.1002/ijgo.71421 [doi] AB - OBJECTIVE: Stress urinary incontinence (SUI) is a prevalent and costly condition, with an estimated lifetime risk of surgery reaching 20% by the age of 80 years. Single-incision mini-slings (SIMS) represent the latest advancement in midurethral sling technology, offering a minimally invasive alternative to traditional retropubic and transobturator tapes (TOT). Studies indicate comparable subjective and objective cure rates between SIMS and TOT, with potential advantages in postmenopausal women owing to earlier mobilization and reduced morbidity. The study aimed to evaluate the efficacy and safety of SIMS under local anesthesia in the management of SUI. METHODS: A prospective cohort study was conducted in a tertiary academic urogynecology unit from December 2019 to December 2024. Consecutive patients meeting the inclusion criteria were enrolled following a standardized diagnostic and treatment protocol. Informed consent was obtained, and ethical approval was granted by the institutional review board. All patients underwent SIMS placement (Altis(c), Coloplast) performed by two experienced urogynecologists under local anesthesia. Sling tensioning was performed via a standardized cough stress test at bladder volumes equal to normal voiding volume, as per pre-operative urodynamics. Patients were followed for 3 weeks and 12 months post-surgery. Data analysis was conducted using Microsoft Excel. Descriptive statistics were applied, and paired t-tests compared pre- and post-operative results. Statistical significance was set at P < 0.05. RESULTS: A total of 124 women underwent SIMS for SUI, with a mean age of 63.8 +/- 11.1 years and a body mass index (BMI, calculated as weight in kilograms divided by the square of height in meters) of 29.0 +/- 4.3. Among them, 21.8% had severe and 78.2% had moderate SUI, while 30.6% presented with mixed urinary incontinence (MUI). Pre-operatively, mean International Consultation on Incontinence Questionnaire (ICIQ) Urinary Incontinence Short Form (ICIQ-UI-SF) and Female Lower Urinary Tract Symptoms (ICIQ-FLUTS) scores were 14.3 +/- 2.4 and 19.0 +/- 6.0, respectively. All surgeries were performed in an ambulatory setting, with 95.2% discharged the same day. The mean surgical time was 31.5 +/- 8.0 min, and peri-operative pain was 2.8 +/- 1.8/10. At discharge, all but one patient had a negative stress test, and 99.2% would recommend the procedure. At 12 months, the composite outcome was successful for 103 (83.1%) patients, with an objective cure rate of 94.3% and a subjective cure rate of 83.1%. A clinically important difference in ICIQ-FLUTS was observed in 92.7% of cases. Patient Global Impression of Improvement (PGI-I, scores 1 or 2) was reported by 93.5%, and Patient Global Impression of Severity (PGI-S, score 1) was reported by 83.9%. There were no cases of bladder perforation, major bleeding, mesh erosion, or reoperation. CONCLUSION: The present study evaluates SIMS under local anesthesia as a minimally invasive and effective treatment for SUI, highlighting its potential benefits in post-operative recovery and resource optimization. The findings contribute to the ongoing advancements in SUI management, particularly in addressing modern healthcare challenges. CI - (c) 2026 International Federation of Gynecology and Obstetrics. FAU - Tsiapakidou, Sofia AU - Tsiapakidou S AUID- ORCID: 0000-0001-8992-1954 AD - 1st Department of Obstetrics and Gynecology, Aristotle University of Thessaloniki, Papageorgiou General Hospital, Thessaloniki, Greece. FAU - Theodoulidis, Iakovos AU - Theodoulidis I AD - Unit of Urogynecology, 1st Department of Obstetrics and Gynecology, Aristotle University Hospital, Papageorgiou General Hospital, Thessaloniki, Greece. FAU - Grimbizis, Grigoris AU - Grimbizis G AD - 1st Department of Obstetrics and Gynecology, Aristotle University of Thessaloniki, Papageorgiou General Hospital, Thessaloniki, Greece. FAU - Mikos, Themistoklis AU - Mikos T AD - Unit of Urogynecology, 1st Department of Obstetrics and Gynecology, Aristotle University Hospital, Papageorgiou General Hospital, Thessaloniki, Greece. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Int J Gynaecol Obstet JT - International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics JID - 0210174 SB - IM OTO - NOTNLM OT - local anesthesia OT - single-incision mini-slings OT - stress urinary incontinence OT - surgery EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:24 PHST- 2026/09/20 00:00 [revised] PHST- 2025/09/04 00:00 [received] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:24 [entrez] AID - 10.1002/ijgo.71421 [doi] PST - aheadofprint SO - Int J Gynaecol Obstet. 2026 Oct 29. doi: 10.1002/ijgo.71421. PMID- 42954655 OWN - NLM STAT- Publisher LR - 20261029 IS - 1365-2702 (Electronic) IS - 0962-1067 (Linking) DP - 2026 Oct 29 TI - Leadership Development Programmes for Clinical Nurses: A Systematic Review. LID - 10.1111/jocn.70613 [doi] AB - AIM: To provide a comprehensive understanding of the current leadership development programmes for clinical nurses. DESIGN: Systematic review was designed and reported in accordance with the PRISMA and Synthesis Without Meta-analysis (SWiM) guidelines. DATA SOURCES: Four electronic databases from 2014 to 2026 studies published were searched. METHODS: A comprehensive search was conducted across PubMed, Cochrane, Scopus and Web of Science. English, full-text literature from 2014 to 2026. This systematic review was registered with PROSPERO under the number CRD42024587447. RESULTS: A systematic review of 18 studies showed that leadership development programmes significantly improved nurses' relational leadership skills (transformational, paternalistic, authentic, resonant and caring), job satisfaction, organizational commitment and self-efficacy, while reducing emotional exhaustion. For nurse managers, these programmes enhance care management knowledge, build healthier work environments and strengthen the hospital safety climates. Incorporating active learning methods, such as simulations, role-playing, reflective practice and 360 degrees feedback, improves engagement and skill retention. CONCLUSION: Studies show that the conceptual framework of leadership programmes for nurses and nurse managers varies with the skills/behaviours they aim to develop, and there is no standardization regarding the programme duration. However, evidence on their impact on institutional and patient outcomes remains limited. These gaps and variables should be considered when structuring leadership programmes in future research. RELEVANCE TO CLINICAL PRACTICE: Relevant for clinical nurses, nurse managers and healthcare organizations, with potential to enhance retention, professional growth and indirectly patient care quality. There is a lack of standardized leadership development programmes and insufficient evidence on their impact on organizational and patient outcomes. Programmes improved nurses' leadership competencies, commitment, satisfaction and self-efficacy, while reducing exhaustion; nurse managers gained caring and authentic leadership skills. REPORTING METHOD: The reporting method, encompassing data selection, extraction and synthesis, adheres to the PRISMA guidelines and the SWiM framework to ensure methodological transparency, clarity and reproducibility. PATIENT OR PUBLIC CONTRIBUTION: No patient or public contribution. TRIAL REGISTRATION: This systematic review was registered with PROSPERO under number CRD42024587447. CI - (c) 2026 John Wiley & Sons Ltd. FAU - Gokalp, Damla AU - Gokalp D AUID- ORCID: 0000-0002-7785-0578 AD - Department of Nursing, Faculty of Health Sciences, Kutahya Health Sciences University, Kutahya, Turkiye. FAU - Yesildere Saglam, Havva AU - Yesildere Saglam H AUID- ORCID: 0000-0001-8311-3745 AD - Department of Nursing, Faculty of Health Sciences, Kutahya Health Sciences University, Kutahya, Turkiye. FAU - Gursoy, Elif AU - Gursoy E AUID- ORCID: 0000-0003-0147-8682 AD - Department of Nursing, Faculty of Health Sciences, Eskisehir Osmangazi University, Eskisehir, Turkiye. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - J Clin Nurs JT - Journal of clinical nursing JID - 9207302 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:16 PHST- 2026/08/11 00:00 [revised] PHST- 2025/10/20 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:16 [entrez] AID - 10.1111/jocn.70613 [doi] PST - aheadofprint SO - J Clin Nurs. 2026 Oct 29. doi: 10.1111/jocn.70613. PMID- 42954652 OWN - NLM STAT- Publisher LR - 20261029 IS - 1542-6270 (Electronic) IS - 1060-0280 (Linking) DP - 2026 Oct 29 TI - From Itch to Innovation: The Emerging Role of Remibrutinib in Chronic Spontaneous Urticaria. PG - 10600280261495540 LID - 10.1177/10600280261495540 [doi] AB - OBJECTIVE: This article reviews the published data encompassing the development, pharmacology, efficacy, and safety of remibrutinib, summarizing data from pivotal clinical studies and clinical relevance in the treatment of chronic spontaneous urticaria. DATA SOURCES: A literature review was conducted in PubMed and ClinicalTrials.gov from inception through August 2026, using terms "RHAPSIDO," "remibrutinib," "LOU064," and "chronic spontaneous urticaria." Articles from reference lists were included to identify potentially relevant literature. STUDY SELECTION AND DATA EXTRACTION: Clinical data and studies were limited to those published in the English language that discussed the safety and efficacy of remibrutinib. DATA SYNTHESIS: The pivotal phase III studies REMIX-1 (n = 470) and REMIX-2 (n = 455) met all primary endpoints at week 12, with Urticaria Activity Score over 7 days (UAS7) reductions of -20.02 and -19.41 versus -13.79 and -11.73 with placebo, parallel improvements in itch and hive severity, and complete absence of itch and hives in 31.1% and 27.9% versus 10.5% and 6.5%. Safety was comparable to placebo, with mild mucocutaneous bleeding (mainly petechiae) the only category significantly more frequent.Relevance to Patient Care and Clinical Practice in Comparison to Existing Drugs:As the first approved oral targeted Bruton's tyrosine kinase (BTK) inhibitor, remibrutinib provides a new therapeutic option in chronic spontaneous urticaria treatment. Clinical practice is improved by the broadened evidence-based management option, which offers an oral pill alternative with no required routine lab monitoring in place of biologic injections. CONCLUSION: Remibrutinib provides a new treatment option with the ability to control symptoms and improve quality of life for patients with difficult-to-treat chronic spontaneous urticaria. FAU - Huston, Jessica AU - Huston J AUID- ORCID: 0000-0002-0860-2108 AD - Department of Pharmacy Education and Practice, College of Pharmacy, University of Florida, Jacksonville, USA. FAU - Carpenter, Lauren AU - Carpenter L AUID- ORCID: 0009-0004-4606-2511 AD - Department of Pharmacy Education and Practice, College of Pharmacy, University of Florida, Jacksonville, USA. FAU - Corona, Kelli AU - Corona K AUID- ORCID: 0009-0008-4738-7392 AD - Department of Pharmacy Education and Practice, College of Pharmacy, University of Florida, Jacksonville, USA. FAU - Deeb, Alexander AU - Deeb A AD - Sidney Kimmel Medical College, Philadelphia, PA, USA. FAU - Deeb, Elias AU - Deeb E AD - PCOM South Georgia, Moultrie, USA. FAU - Ashchi, Andrew AU - Ashchi A AD - East Coast Institute for Research, Jacksonville, FL, USA. FAU - Olabuntu, Mariam AU - Olabuntu M AD - Department of Pharmacy Education and Practice, College of Pharmacy, University of Florida, Jacksonville, USA. FAU - Asadi, Adam AU - Asadi A AD - Florida State University, Tallahassee, USA. FAU - Patton, Inara AU - Patton I AD - Jefferson Torresdale Hospital, Philadelphia, PA, USA. FAU - Offiah, Maureen AU - Offiah M AD - Dermatology West: Hattiesburg Clinic, MS, USA. FAU - Goldfaden, Rebecca AU - Goldfaden R AUID- ORCID: 0000-0002-6401-4888 AD - Rovia Clinical Research, Jacksonville, FL, USA. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - United States TA - Ann Pharmacother JT - The Annals of pharmacotherapy JID - 9203131 SB - IM OTO - NOTNLM OT - LOU064 OT - RHAPSIDO OT - chronic spontaneous urticaria OT - remibrutinib EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:16 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:16 [entrez] AID - 10.1177/10600280261495540 [doi] PST - aheadofprint SO - Ann Pharmacother. 2026 Oct 29:10600280261495540. doi: 10.1177/10600280261495540. PMID- 42954648 OWN - NLM STAT- Publisher LR - 20261029 IS - 1532-7604 (Electronic) IS - 1088-8705 (Linking) DP - 2026 Oct 29 TI - The Ferret as a Companion Animal: A Welfare Analysis of Husbandry, Health and Behavior Across Regions. PG - 1-17 LID - 10.1080/10888705.2026.2753141 [doi] AB - Regional variation in companion-ferret management and owner-reported welfare indicators is poorly documented. We compared acquisition, nutrition, housing, behavior, health, preventive care, and reproductive management across Slovakia/Czech Republic (SK/CZ), the United States/Canada (USA/CAN), and other countries (O-C) using 422 owner questionnaires (one per ferret). Acquisition source, feeding, housing, preventive care, behavior, and reproductive management differed among groups. Breeders were the most common acquisition source in SK/CZ and O-C, whereas pet shops predominated in USA/CAN. Biting was reported most often in SK/CZ, whereas "exploratory" and "friendly" were selected most often in USA/CAN. Diagnosed adrenal disease was reported most frequently in USA/CAN. Age > 4 years (adjusted OR = 3.85, 95% CI: 1.99-7.44) and USA/CAN membership (adjusted OR = 5.06, 95% CI: 1.67-15.37) were associated with higher odds of reported adrenal disease; neutering status was not independently associated (adjusted OR = 1.73, 95% CI: 0.42-7.02). Findings demonstrate geographical differences in ferret management and selected owner-reported health and behavioral indicators but do not establish causality or overall welfare rankings. FAU - Kottferova, Lucia AU - Kottferova L AD - Clinic of Birds, Exotic and Free Living Animals, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovakia. FAU - Sinkova, Ester AU - Sinkova E AD - Department of Public Veterinary Medicine and Animal Welfare, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovakia. FAU - Skurkova, Lenka AU - Skurkova L AD - Department of Public Veterinary Medicine and Animal Welfare, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovakia. FAU - Dudra Kasicova, Zuzana AU - Dudra Kasicova Z AD - Department of Public Veterinary Medicine and Animal Welfare, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovakia. FAU - Florian, Martin AU - Florian M AD - Department of Public Veterinary Medicine and Animal Welfare, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovakia. FAU - Meznikova, Sarah AU - Meznikova S AD - Department of Public Veterinary Medicine and Animal Welfare, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovakia. FAU - Kottferova, Jana AU - Kottferova J AD - Department of Public Veterinary Medicine and Animal Welfare, University of Veterinary Medicine and Pharmacy in Kosice, Kosice, Slovakia. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - J Appl Anim Welf Sci JT - Journal of applied animal welfare science : JAAWS JID - 9804404 SB - IM OTO - NOTNLM OT - Ferret OT - animal welfare OT - behavior OT - geographical variation OT - husbandry EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:16 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:16 [entrez] AID - 10.1080/10888705.2026.2753141 [doi] PST - aheadofprint SO - J Appl Anim Welf Sci. 2026 Oct 29:1-17. doi: 10.1080/10888705.2026.2753141. PMID- 42954642 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5207 (Electronic) IS - 1520-5207 (Linking) VI - 130 IP - 43 DP - 2026 Oct 29 TI - Influence of Electric Fields in Water Microdroplets and Organic Molecules: A Comparative Study of Surface and Internal Environments. PG - 11493-11501 LID - 10.1021/acs.jpcb.6c03799 [doi] AB - Chemical reactions in water microdroplets can proceed orders of magnitude faster than in bulk water, yet the molecular origins of this behavior remain unclear. Increasing evidence points to strong and heterogeneous electric fields at droplet interfaces as a key factor, but how different functional groups couple to these fields horizontal line and which bonds are most susceptible to field-induced polarization horizontal line remains poorly understood. In this work, we use polarizable molecular dynamics (MD) simulations to quantify local electric fields in and on a 3 nm water microdroplet model and their influence on two representative solute molecules, protonated 5,5-dimethyl-1-pyrroline-N-oxide (DMPO-H) and 1-methylcyclopentanol (MCP), positioned at the interface and in the interior. We projected electric fields onto intramolecular bond vectors to evaluate bond-level sensitivity to microenvironmental heterogeneity. Simulations indicate that the droplet interface produces stronger, broader, and more asymmetric electric field distributions than the interior. Polar and solvent-exposed bonds horizontal line particularly O-H bonds in both molecules and C-C and C-O bonds in MCP horizontal line exhibit the largest fluctuations and most frequent extreme field events, whereas less polar or internally oriented bonds (e.g., O-N and N-C in DMPO-H) show narrower, symmetric distributions largely independent of location. These results demonstrate that electric field effects in microdroplets are strongly dependent on bond type, orientation, and local environment, providing a molecular-level framework for understanding how interfacial electric fields selectively polarize functional groups and may influence field-sensitive reactivity in confined aqueous systems. CI - (c) 2026 UChicgo Argonne, LLC, Operator of Argonne National Laboratory. Published by American Chemical Society. FAU - Li, Shi AU - Li S AUID- ORCID: 0000-0003-0505-6751 AD - Materials Science Division, Argonne National Laboratory, Lemont, Illinois60439, United States. FAU - Asserghine, Abdelilah AU - Asserghine A AD - Department of Chemistry, University of Illinois Urbana-Champaign, Urbana, Illinois61801, United States. FAU - Rodriguez-Lopez, Joaquin AU - Rodriguez-Lopez J AUID- ORCID: 0000-0003-4346-4668 AD - Department of Chemistry, University of Illinois Urbana-Champaign, Urbana, Illinois61801, United States. FAU - Assary, Rajeev Surendran AU - Assary RS AUID- ORCID: 0000-0002-9571-3307 AD - Materials Science Division, Argonne National Laboratory, Lemont, Illinois60439, United States. LA - eng GR - DE-SC0024892/Office of Science/ PT - Journal Article PL - United States TA - J Phys Chem B JT - The journal of physical chemistry. B JID - 101157530 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:16 PHST- 2026/06/09 00:00 [received] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/08/21 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:16 [entrez] AID - 5454637 [pii] AID - 10.1021/acs.jpcb.6c03799 [doi] PST - ppublish SO - J Phys Chem B. 2026 Oct 29;130(43):11493-11501. doi: 10.1021/acs.jpcb.6c03799. PMID- 42954636 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1520-5207 (Electronic) IS - 1520-5207 (Linking) VI - 130 IP - 43 DP - 2026 Oct 29 TI - Molecular Origins of Catalysis in Random Heteropolymer Enzyme Mimics through All-Atom Simulations. PG - 11657-11666 LID - 10.1021/acs.jpcb.6c05638 [doi] AB - Random heteropolymers (RHPs) have recently emerged as synthetic macromolecules capable of enzyme-inspired catalysis despite lacking defined secondary and tertiary structures. However, the molecular origins of their catalytic activity remain less understood. Here, we use all-atom molecular dynamics simulations to investigate a methacrylate-based RHP shown to experimentally catalyze the selective cyclization of citronellal. Despite its high anionic monomer content, the polymer self-assembles into molten globules with heterogeneous surfaces due to high hydrophobic content. This dynamic organization creates chemically distinct binding interfaces where substrates encounter local environments enriched in specific monomers. Anionic sulfopropyl methacrylate groups preferentially associate with protonated intermediates, providing electrostatic stabilization relevant to catalysis. Free energy calculations reveal a reduced barrier for substrate conformational closure that promotes reactive geometries in hydrophobic environments, relating to hydrophobic patches found on the RHP. We further observe selective adsorption behavior when multiple substrates are present, with isopulegol desorbing more rapidly than citronellal or menthoglycol, consistent with favorable product release and catalytic turnover. Together, these findings demonstrate that RHPs without rigid folds can create dynamic, chemically heterogeneous microenvironments shaped by monomer composition and sequence, enabling electrostatic and geometric stabilization, providing a molecular rationale for their experimentally observed catalytic function. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Hilburg, Shayna L AU - Hilburg SL AD - Department of Materials Science and Engineering, MIT, Cambridge, Massachusetts02139, United States. AD - Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland20899, United States. FAU - Han, Zexiang AU - Han Z AUID- ORCID: 0000-0002-8968-0860 AD - Department of Materials Science and Engineering, MIT, Cambridge, Massachusetts02139, United States. AD - Yusuf Hamied Department of Chemistry, University of Cambridge, CambridgeCB2 1EW, U.K. FAU - Jin, Tianyi AU - Jin T AUID- ORCID: 0000-0002-9974-2041 AD - Department of Materials Science and Engineering, MIT, Cambridge, Massachusetts02139, United States. AD - Department of Chemical Engineering, MIT, Cambridge, Massachusetts02139, United States. AD - Division of Chemistry and Chemical Engineering, Caltech, Pasadena, California91125, United States. FAU - Ganesh, Priya AU - Ganesh P AD - Department of Materials Science and Engineering, MIT, Cambridge, Massachusetts02139, United States. FAU - Yu, Hao AU - Yu H AUID- ORCID: 0000-0002-1594-769X AD - Department of Materials Science and Engineering, University of California, Berkeley, Berkeley, California94704, United States. AD - Department of Chemistry, University of California, Berkeley, Berkeley, California94704, United States. FAU - Kang, Philjun AU - Kang P AD - Department of Chemistry, University of California, Berkeley, Berkeley, California94704, United States. FAU - Xu, Ting AU - Xu T AUID- ORCID: 0000-0002-2831-2095 AD - Department of Materials Science and Engineering, University of California, Berkeley, Berkeley, California94704, United States. AD - Department of Chemistry, University of California, Berkeley, Berkeley, California94704, United States. AD - Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, California94704, United States. AD - Kavli Energy NanoScience Institute, University of California, Berkeley, Berkeley, California94704, United States. FAU - Alexander-Katz, Alfredo AU - Alexander-Katz A AD - Department of Materials Science and Engineering, MIT, Cambridge, Massachusetts02139, United States. LA - eng GR - EFRI E3P 2132025/National Science Foundation/ GR - NA/NIST/NIST DOC/United States GR - HDTRA11910011/Defense Threat Reduction Agency/ GR - NA/Imperial-MIT International Research Opportunities Program/ GR - NA/Michael and Sonja Koerner Chair/ GR - NA/MIT Undergraduate Research Opportunities Program/ PT - Journal Article PL - United States TA - J Phys Chem B JT - The journal of physical chemistry. B JID - 101157530 RN - 0 (Polymers) RN - 0 (Acyclic Monoterpenes) RN - QB99VZZ7GZ (citronellal) RN - 0 (Aldehydes) RN - 0 (Monoterpenes) RN - 0 (Methacrylates) SB - IM MH - *Molecular Dynamics Simulation MH - *Polymers/chemistry MH - Catalysis MH - Acyclic Monoterpenes MH - Hydrophobic and Hydrophilic Interactions MH - Aldehydes/chemistry MH - Monoterpenes/chemistry MH - Methacrylates/chemistry MH - Static Electricity MH - Thermodynamics MH - Cyclization EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:16 PHST- 2026/08/19 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:16 [entrez] AID - 5453383 [pii] AID - 10.1021/acs.jpcb.6c05638 [doi] PST - ppublish SO - J Phys Chem B. 2026 Oct 29;130(43):11657-11666. doi: 10.1021/acs.jpcb.6c05638. PMID- 42954634 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5207 (Electronic) IS - 1520-5207 (Linking) VI - 130 IP - 43 DP - 2026 Oct 29 TI - Composition-Controlled Hydrogen-Bond Restructuring and Interfacial Transport in Glycerol-Ethanol Mixtures for Orange Essential Oil Separation: Experiments and Multiscale Modeling. PG - 11568-11586 LID - 10.1021/acs.jpcb.6c04867 [doi] AB - Hydrogen-bonded glycerol-ethanol (Gly-EtOH) mixtures are promising biodiesel-derived solvents for orange essential oil (OEO) separation. However, how solvent composition governs equilibrium and transport properties and selectivity remains unclear. We combined rotational rheometry, ReaxFF molecular dynamics (ReaxFF-MD), density functional theory (DFT), COSMO-RS, Redlich-Kwong equation of state (RK EoS), and machine learning to establish structure-property-separation relationships. ReaxFF-MD and RK EoS reproduced the composition-dependent density behavior with AARDs of 4.6% and 2.0%, respectively. Rheometry validated the ReaxFF-MD-predicted decrease in viscosity with increasing ethanol content. ReaxFF-MD and DFT reveal that ethanol reorganizes the glycerol-dominated hydrogen-bond network by altering O...O coordination and O...H correlations, while COSMO-RS reveals a composition-dependent shift toward weakly polarized surface segments. Polynomial ridge regression and Gaussian process regression were used to interpolate density and viscosity, respectively. Source-separated and grouped cross-validation showed that composition interpolation was more reliable than prediction at a completely unseen temperature. Interfacial MD simulations further indicate that solute-solvent affinity, interfacial organization, and diffusivity contrast jointly govern linalool enrichment, with the highest simulated selectivity at x1 = 0.5. These findings establish a composition-controlled mechanism connecting hydrogen-bond restructuring, bulk transport, and interfacial separation, enabling rational design of sustainable biomass-derived solvents. CI - (c) 2026 American Chemical Society. FAU - Cai, Guoxiao AU - Cai G AUID- ORCID: 0009-0008-1390-5841 AD - Department of Renewable Energy Science and Engineering, School of Electrical and Information Engineering, Tongling University, Tongling244061, China. AD - College of Chemical Engineering, Xiangtan University, Xiangtan411105, China. AD - Department of Chemical Engineering, Faculty of Engineering, Kyoto University, Kyoto-Daigaku-Katsura, Nishikyo-ku, Kyoto615-8530, Japan. FAU - Zhang, Ruiyao AU - Zhang R AD - School of Artificial Intelligence, Tongling University, Tongling244061, China. FAU - Qiu, Fuqiang AU - Qiu F AD - Department of Renewable Energy Science and Engineering, School of Electrical and Information Engineering, Tongling University, Tongling244061, China. FAU - Xiong, Wei AU - Xiong W AD - College of Chemical Engineering, Xiangtan University, Xiangtan411105, China. FAU - Zhou, Songlin AU - Zhou S AD - Department of Renewable Energy Science and Engineering, School of Electrical and Information Engineering, Tongling University, Tongling244061, China. FAU - Hao, Fang AU - Hao F AUID- ORCID: 0000-0002-3143-0359 AD - College of Chemical Engineering, Xiangtan University, Xiangtan411105, China. FAU - Kong, Chang Yi AU - Kong CY AUID- ORCID: 0000-0001-6465-2006 AD - Department of Environment and Energy System, Graduate School of Science and Technology, Shizuoka University, 3-5-1 Johoku Chuo-ku, Hamamatsu432-8561, Japan. LA - eng GR - 2025AHGXZK40593/Anhui Provincial Department of Education/ GR - 2025aijy358/Anhui Provincial Department of Education/ GR - 2025tlxyrc084/Tongling University/ PT - Journal Article PL - United States TA - J Phys Chem B JT - The journal of physical chemistry. B JID - 101157530 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:15 PHST- 2026/07/20 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/09/17 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:15 [entrez] AID - 5452501 [pii] AID - 10.1021/acs.jpcb.6c04867 [doi] PST - ppublish SO - J Phys Chem B. 2026 Oct 29;130(43):11568-11586. doi: 10.1021/acs.jpcb.6c04867. PMID- 42954622 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5207 (Electronic) IS - 1520-5207 (Linking) VI - 130 IP - 43 DP - 2026 Oct 29 TI - From Hydration Shell to Chelate Cavity: Funnel-Metadynamics Reveals the Sr2+ Chelation Pathway of Decorporol. PG - 11502-11514 LID - 10.1021/acs.jpcb.6c03989 [doi] AB - The selective binding of Sr2+ over Ca2+ is very important for the development of chelating agents capable of selectively targeting radiostrontium in biological and environmental settings. Explicit-solvent molecular dynamics, Funnel-Metadynamics (Fun-MetaD), and density functional theory calculations were applied to resolve the molecular pathway of Ca2+ and Sr2+ binding by EDTA, Kryptofix 22, and Decorporol. EDTA was used as a benchmark system to validate the reparameterized force field protocol based on Minimal Basis Iterative Stockholder partial charges. The resulting relative free energy profiles reproduced the expected stronger stabilization of Ca2+ than Sr2+ by EDTA. Kryptofix 22 was included as an unfunctionalized diaza-18-crown-6 reference. Fun-MetaD profiles did not show a deeply stabilized contact-bound state. Higher concentration ITC measurements revealed weak, entropy-dominated Sr2+ association, while no reliable Ca2+ binding could be extracted under the same conditions. In contrast, simulations with Decorporol displayed a deeper contact-bound minimum for Sr2+, with minimum-to-bulk relative free energy differences of -113.2 +/- 9.2 kJ/mol for Sr2+ compared with -98.2 +/- 9.0 kJ/mol for Ca2+. Bound state integration and standard-state correction yielded binding free energies of -101.1 +/- 2.1 kJ/mol for Sr2+ and -87.6 +/- 6.4 kJ/mol for Ca2+, retaining the Sr2+-favoring trend. The simulations reveal that Decorporol binding proceeds through a two-step mechanism involving a solvent-shared ion-pair (SIP) intermediate followed by the formation of a deeply bound contact ion-pair (CIP) complex. The solvent-shared intermediate is not Sr2+-selective as both the Fun-MetaD profiles and density functional theory (DFT) calculations indicate similar Ca2+ and Sr2+ stability in this state. Selectivity emerges during the transition to the CIP complex, where the ion must undergo total dehydration to enter the Decorporol cavity. As Sr2+ has a more labile hydration shell, it can form the dehydrated contact-bound state more readily than Ca2+. Thus, Decorporol selectivity arises mainly from dehydration-gated stabilization of the CIP complex, rather than from initial ion recognition in the SIP state. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Horvath, Tamas AU - Horvath T AUID- ORCID: 0009-0003-8246-1125 AD - Institute of Chemistry, University of Miskolc, Egyetemvaros A/2, H-3515Miskolc, Hungary. AD - Higher Education and Industrial Cooperation Centre, University of Miskolc, H-3515Miskolc, Hungary. FAU - Szori, Milan AU - Szori M AUID- ORCID: 0000-0003-4895-0999 AD - Institute of Chemistry, University of Miskolc, Egyetemvaros A/2, H-3515Miskolc, Hungary. FAU - Viskolcz, Bela AU - Viskolcz B AUID- ORCID: 0000-0002-0777-9569 AD - Institute of Chemistry, University of Miskolc, Egyetemvaros A/2, H-3515Miskolc, Hungary. AD - Higher Education and Industrial Cooperation Centre, University of Miskolc, H-3515Miskolc, Hungary. FAU - Rozsa, Zsofia Borbala AU - Rozsa ZB AD - Institute of Chemistry, University of Miskolc, Egyetemvaros A/2, H-3515Miskolc, Hungary. AD - Department of Biochemistry, Eotvos Lorand University, Pazmany Peter stny 1/c, BudapestH-1117, Hungary. LA - eng GR - RRF-2.3.1-21-2022-00014/European Commission/ GR - RRF-2.3.1-21-2022-00014/Nemzeti Kutat?si Fejleszt?si ls Innov?ci?s Hivatal/ GR - TKP2021-NVA-14/Nemzeti Kutat?si Fejleszt?si ls Innov?ci?s Hivatal/ PT - Journal Article PL - United States TA - J Phys Chem B JT - The journal of physical chemistry. B JID - 101157530 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:15 PHST- 2026/06/17 00:00 [received] PHST- 2026/08/17 00:00 [accepted] PHST- 2026/08/06 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:15 [entrez] AID - 5450799 [pii] AID - 10.1021/acs.jpcb.6c03989 [doi] PST - ppublish SO - J Phys Chem B. 2026 Oct 29;130(43):11502-11514. doi: 10.1021/acs.jpcb.6c03989. PMID- 42954615 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5215 (Electronic) IS - 1089-5639 (Linking) VI - 130 IP - 43 DP - 2026 Oct 29 TI - Vibrational-Gated Intersystem Crossing in Phenolphthalein Dianion: A TDDFT Insight into the Fluorescence Quenching Mechanism. PG - 8570-8575 LID - 10.1021/acs.jpca.6c04683 [doi] AB - The structural rigidity of molecular frameworks plays a pivotal role in modulating intersystem crossings (ISCs). Here, we systematically investigate the distinct fluorescence behaviors of the phenolphthalein dianion (PHP2-) and the fluorescein dianion (FLU2-) horizontal line two structurally analogous xanthene derivatives. By employing time-dependent density functional theory (TDDFT), our results reveal that, although both dianions possess nearly degenerate 1pipi* and 3npi* states with comparable spin-orbital couplings ( approximately 37 cm-1) in the Franck-Condon region, the subsequent relaxation pathways are dictated by distinct vibrational modes. In PHP2-, the twisting of a specific phenol ring serves as the critical vibrational gate that funnels the initially populated 1pipi* state to the 3npi* state. This is followed by a barrierless internal conversion from 3npi* to the lowest 3pipi* state, ultimately leading to complete fluorescence quenching. In stark contrast, in FLU2-, the coplanar xanthene core rigidified by an oxygen bridge effectively suppresses this twisting motion. The available torsional modes drive the system toward its 1pipi* minimum, thereby precluding ISC and allowing efficient radiative decay. These findings establish a clear vibrational-mode-dependent ISC mechanism, demonstrating that molecular rigidity does not simply alter energy gaps but rather selectively eliminates the specific nuclear motion required to activate ISC. CI - (c) 2026 American Chemical Society. FAU - Li, Lu AU - Li L AUID- ORCID: 0009-0009-6521-3481 AD - Department of Criminal Science and Technology, Liaoning Police College, Dalian116036, China. FAU - Xu, Xiuming AU - Xu X AD - Department of Criminal Science and Technology, Liaoning Police College, Dalian116036, China. FAU - Guan, YuXin AU - Guan Y AD - Department of Criminal Science and Technology, Liaoning Police College, Dalian116036, China. FAU - Sun, Xue AU - Sun X AD - Department of Criminal Science and Technology, Liaoning Police College, Dalian116036, China. FAU - Tang, FengMei AU - Tang F AD - Department of Criminal Science and Technology, Liaoning Police College, Dalian116036, China. FAU - Luo, Jian AU - Luo J AUID- ORCID: 0000-0002-3928-793X AD - School of Physics and Optoelectronic Engineering, Yangtze University, Jingzhou434023, China. LA - eng GR - 2024LNPCBS-106/Liaoning Police College/ GR - 2025-MSLH-338/Liaoning Provincial Science and Technology Program Joint Fund/ PT - Journal Article PL - United States TA - J Phys Chem A JT - The journal of physical chemistry. A JID - 9890903 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:15 PHST- 2026/07/14 00:00 [received] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:15 [entrez] AID - 5453400 [pii] AID - 10.1021/acs.jpca.6c04683 [doi] PST - ppublish SO - J Phys Chem A. 2026 Oct 29;130(43):8570-8575. doi: 10.1021/acs.jpca.6c04683. PMID- 42954605 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5215 (Electronic) IS - 1089-5639 (Linking) VI - 130 IP - 43 DP - 2026 Oct 29 TI - Symmetry- and Anharmonicity-Controlled Intramolecular Vibrational Energy Flow in Isolated Nitromethane: Ab Initio Molecular Dynamics Simulations. PG - 8545-8556 LID - 10.1021/acs.jpca.6c03084 [doi] AB - Mode-specific intramolecular vibrational energy redistribution is a key elementary process in the earliest molecular response of energetic materials, yet a quantitative, mode-resolved description of its microscopic driving forces remains challenging. Here we combine density functional theory (DFT), ab initio molecular dynamics (AIMD), normal-mode projection, spectral energy density analysis (SED), and a perturbative mode-coupling model to resolve vibrational energy flow in isolated nitromethane after selective excitation of individual normal modes. The simulations reveal a nonrandom, hierarchical energy-flow network in which low- and midfrequency modes funnel energy toward a small set of accepting modes. Spectral fingerprints of acceptor-mode momenta show that most primary transfer channels are not fundamental-fundamental resonances but overtone-fundamental resonances mediated by cubic anharmonicity. This mechanism naturally explains apparently cross-symmetry transfer: the overtone of an A'' mode transforms as A' and can therefore couple to A' acceptor fundamentals. A simplified Fermi's Golden Rule model, parametrized by bilinear and third-order anharmonic force constants and finite-time Lorentzian detuning factors, predicts the dominant primary acceptors observed in AIMD. The isolated molecule exhibits coherent quantum-beating-like energy oscillations whose periods are governed by the detuning of the corresponding resonance condition. These results recast vibrational energy transfer in nitromethane as a symmetry-filtered anharmonic resonance network and provide a transferable framework for identifying early energy-flow bottlenecks in energetic molecules. CI - (c) 2026 American Chemical Society. FAU - Zhao, Juan AU - Zhao J AUID- ORCID: 0000-0003-4206-8313 AD - Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Molecular Reaction Dynamics Laboratory, Institute of Chemistry, Chinese Academy of Sciences, Beijing100190, P. R. China. AD - University of Chinese Academy of Sciences, Beijing100049, P. R. China. FAU - Wang, Jianping AU - Wang J AUID- ORCID: 0000-0001-7127-869X AD - Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Molecular Reaction Dynamics Laboratory, Institute of Chemistry, Chinese Academy of Sciences, Beijing100190, P. R. China. AD - University of Chinese Academy of Sciences, Beijing100049, P. R. China. LA - eng GR - 21327802/National Natural Science Foundation of China (NSFC)/ GR - 21573243/National Natural Science Foundation of China (NSFC)/ GR - 21603238/National Natural Science Foundation of China (NSFC)/ PT - Journal Article PL - United States TA - J Phys Chem A JT - The journal of physical chemistry. A JID - 9890903 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:15 PHST- 2026/05/12 00:00 [received] PHST- 2026/08/17 00:00 [accepted] PHST- 2026/08/16 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:15 [entrez] AID - 5328229 [pii] AID - 10.1021/acs.jpca.6c03084 [doi] PST - ppublish SO - J Phys Chem A. 2026 Oct 29;130(43):8545-8556. doi: 10.1021/acs.jpca.6c03084. PMID- 42954598 OWN - NLM STAT- Publisher LR - 20261029 IS - 1463-4201 (Print) IS - 1463-4201 (Linking) VI - 0 IP - 0 DP - 2026 Oct 29 TI - Grammetry and Photogrammetry as impression tools in fullarch implant prostheses: is it a magical wand or a myth? A comprehensive critical review. PG - 0 LID - 10.3290/j.ijcd.b7143443 [doi] AB - INTRODUCTION: Various technologies and techniques have been introduced to acquire implant position, orientation, and angulations, especially for completely edentulous full-arch implants. Intraoral and extraoral optical scanners, extraoral photogrammetry, grammetry and finally intraoral photogrammetry are different data acquisition methods for the fabrication of full-arch implant prostheses. Each of the previously mentioned methods depends on different scanning workflows, scan device designs, software manipulation of the acquired data, and post-scanning workflows, resulting in different degrees of accuracy. DATA: Data collection was performed using the following keywords: Extraoral Photogrammetry, Intraoral Photogrammetry, Grammetry with particular attention to clinical trials and systematic reviews. Sources and Study selection: A comprehensive electronic search was conducted on PubMed, Scopus, and Web of science data bases. Articles published until 2025 were reviewed with a focus on clinical studies discussing each data acquisition workflow, software manipulation of each technology, and its relative effect on accuracy. CONCLUSIONS: Conventional implant open tray impression will remain the gold standard; either intraoral optical scanning or photogrammetry have inherent inaccuracies in the workflow itself. Intra-oral photogrammetry may be a game changer after critical clinical research and applications, as it solves the problems of alignment and superimposition inaccuracies. Future research should prioritize clinical studies on the inherent errors of each workflow and how to avoid. CLINICAL RELEVANCE: A full understanding of the underlying technology and workflow of each full-arch data acquisition technique will maximize the benefits of these recently proposed technologies. Therefore, this review aims to aid prosthodontists and clinicians in their selection process of digital acquisition methodology to be utilized in their full arch implant/prostheses cases. It also investigates the recent articles in the literature and highlights the gaps in the literature. FAU - Abdelaziz, Medhat S AU - Abdelaziz MS FAU - Osman, Reham B AU - Osman RB LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Int J Comput Dent JT - International journal of computerized dentistry JID - 100891504 SB - IM OTO - NOTNLM OT - Intraoral photogrammetry OT - full arch implant OT - grammetry OT - intraoral optical scanning OT - photogrammetry EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:15 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:15 [entrez] AID - 7143443 [pii] AID - 10.3290/j.ijcd.b7143443 [doi] PST - aheadofprint SO - Int J Comput Dent. 2026 Oct 29;0(0):0. doi: 10.3290/j.ijcd.b7143443. PMID- 42954590 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Isotope Tuning of Fermi Resonance and Vibrational Hybridization in Surface-Adsorbed CO2 Probed by Tip-Enhanced Raman Spectroscopy. PG - 45942-45951 LID - 10.1021/jacs.6c14567 [doi] AB - Fermi resonance in CO2 serves as a prototypical example of anharmonic vibrational coupling, where the fundamental vibrational mode of C-O symmetric stretching (v1) mixes quantum-mechanically with the nearly degenerate overtone (2v2) of C-O bending, resulting in energy level splitting, spectral peak shifting, intensity redistribution, and band bifurcation in vibrational spectra. Here, we carry out a comprehensive investigation of this cubic anharmonic interaction in surface-adsorbed CO2 clusters using low-temperature ultrahigh-vacuum Tip-Enhanced Raman Spectroscopy (TERS), in combination with isotope tuning. In addition to clear observation of well-resolved Fermi dyads for both C16O2 and C18O2 isotopologues, TERS enables direct observation of the Raman-inactive bending mode (v2) by breaking the conventional Raman selection rule. As a result, comprehensive and important Fermi resonance parameters of CO2 can be determined from the frequency domain directly, including, e.g., the frequency detuning, the Fermi coupling strength, and the mixing angle for the vibrational hybridization. Furthermore, isotope substitution from C16O2 to C18O2 is found to reduce and even reverse the frequency detuning, which modifies the quantum mixing significantly through the change in the mixing angle and leads to a redistribution of vibrational character between the Fermi dyad states. Such an isotope-induced shift in the mixing angle modifies the balance between constructive and destructive interference, resulting in the observed intensity asymmetry of the Fermi dyads. In addition, these Fermi dyads are found to be somewhat sensitive to cluster morphologies and local adsorption environment. These results demonstrate that TERS is a powerful tool for quantitatively probing anharmonic vibrational interactions such as Fermi resonance at the nanoscale, while isotope substitution can provide an effective route to tune vibrational hybridization and quantum interference for molecules adsorbed on surfaces. CI - (c) 2026 American Chemical Society. FAU - Zhang, Zhi-Jing AU - Zhang ZJ AD - Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, Anhui230026, China. FAU - Jia, Wen-Cheng AU - Jia WC AD - School of Electronic Science and Engineering, Xiamen University, Xiamen361005, China. FAU - Yu, Yun-Jie AU - Yu YJ AD - Hefei National Laboratory, University of Science and Technology of China, Hefei, Anhui230088, China. FAU - Jiao, Cheng-Qian AU - Jiao CQ AD - Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, Anhui230026, China. FAU - Dong, Zhi-Peng AU - Dong ZP AD - School of Electronic Science and Engineering, Xiamen University, Xiamen361005, China. FAU - Zhang, Yang AU - Zhang Y AUID- ORCID: 0000-0002-0118-8717 AD - Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, Anhui230026, China. AD - Hefei National Laboratory, University of Science and Technology of China, Hefei, Anhui230088, China. FAU - Zhang, Chao AU - Zhang C AUID- ORCID: 0009-0003-8487-2331 AD - Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, Anhui230026, China. FAU - Zhang, Yao AU - Zhang Y AUID- ORCID: 0000-0002-6524-0289 AD - Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, Anhui230026, China. AD - Hefei National Laboratory, University of Science and Technology of China, Hefei, Anhui230088, China. FAU - Dong, Zhen-Chao AU - Dong ZC AUID- ORCID: 0000-0002-4596-6269 AD - Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, Anhui230026, China. AD - Hefei National Laboratory, University of Science and Technology of China, Hefei, Anhui230088, China. LA - eng GR - 12334018/National Natural Science Foundation of China/ GR - 22572182/National Natural Science Foundation of China/ GR - 22595392/National Natural Science Foundation of China/ GR - 92477133/National Natural Science Foundation of China/ GR - XDB1180000/Chinese Academy of Sciences/ GR - XDB36000000/Chinese Academy of Sciences/ GR - YSBR-054/Chinese Academy of Sciences/ GR - 2016YFA0200601/National Key Research and Development Program of China/ GR - 2021YFA1500500/National Key Research and Development Program of China/ GR - 2021ZD0303301/Innovation Program for Quantum Science and Technology/ GR - KF2025103/Hefei National Research Center for Physical Sciences at the Microscale/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/07/15 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5453559 [pii] AID - 10.1021/jacs.6c14567 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):45942-45951. doi: 10.1021/jacs.6c14567. PMID- 42954588 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Topology-Engineered Dual-Functional COFs with Spatially Cooperated Hydrophilic and Photocatalytic Sites for Harvesting Safe Water from Polluted Atmosphere. PG - 46181-46193 LID - 10.1021/jacs.6c16023 [doi] AB - Atmospheric water harvesting (AWH) has emerged as a promising decentralized solution for freshwater supply in off-grid regions by extracting ubiquitous moisture from the air. However, the atmosphere also carries airborne pathogens and, under extreme scenarios, toxic chemicals, which can be coharvested to contaminate the collected water. Despite extensive efforts to maximize water uptake, the safety of the harvested water has been largely neglected in the pursuit of higher water production yields. Covalent organic frameworks (COFs) provide crystalline, molecularly programmable platforms for integrating water adsorption with photocatalytic detoxification. However, achieving such functional synergy depends on framework topology, which governs site accessibility and mass transport. Here, we report topology-engineered dual-functional COFs that regulate spatial cooperation among hydrophilic sites, photoactive sites, and pore structures in a series of 1D and 2D hydrazone COFs constructed from identical molecular building blocks. Compared to the compact 1D chain packing, the open and continuous channels of the 2D framework increase functional-site accessibility, support hydrogen-bonded water network formation, and facilitate charge-carrier separation and O2 activation. The optimized Por-TAH (2D) exhibits a saturated water uptake of 629 mg g-1, releases 94% of adsorbed water under mild heating, inactivates 99.99% of S. aureus within 1 h, and completely converts chemical agents with high selectivity within 10 min. As a proof-of-concept application, continuous-flow operation demonstrates integrated water harvesting and photocatalytic detoxification, yielding safe water free of detectable mustard gas simulants. This study establishes topology-directed spatial cooperation as a design principle for multifunctional porous materials, enabling safe water production from real contaminated air. CI - (c) 2026 American Chemical Society. FAU - Zhang, Meng AU - Zhang M AD - Frontiers Science Center for High Energy Material, School of Interdisciplinary Science, Beijing Institute of Technology, Beijing100081, China. AD - State Key Laboratory of Transient Chemical Effects and Control, Shaanxi Applied Physics and Chemistry Research Institute, Xi'an710061, China. FAU - Shao, Pengpeng AU - Shao P AD - Hunan Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha410083, P. R. China. FAU - Liu, Jia-Xin AU - Liu JX AD - Chongqing Advanced Materials Institute , Chongqing408107, China. FAU - Wang, Shan AU - Wang S AD - Ministry of Education Key Laboratory of Cluster Science, Beijing Key Laboratory of Intelligent Molecular Materials and High-throughput Manufacturing, Beijing Key Laboratory of Green Hydrogen Energy and Fuel Cells, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing100081, P. R. China. AD - State Key Laboratory of Transient Chemical Effects and Control, Shaanxi Applied Physics and Chemistry Research Institute, Xi'an710061, China. FAU - Wei, Yingying AU - Wei Y AD - Frontiers Science Center for High Energy Material, School of Interdisciplinary Science, Beijing Institute of Technology, Beijing100081, China. FAU - Wang, Qian-You AU - Wang QY AUID- ORCID: 0000-0002-8892-7825 AD - State Key Laboratory of Explosion Science and Safety Protection, Beijing Institute of Technology, Beijing100081, China. AD - Beijing Institute of Technology, Zhengzhou Academy of Intelligent Technology, Zhengzhou450000, China. AD - Beijing Institute of Technology, Chongqing Innovation Center, Chongqing401120, China. FAU - Ma, Qinglang AU - Ma Q AD - Frontiers Science Center for High Energy Material, School of Interdisciplinary Science, Beijing Institute of Technology, Beijing100081, China. AD - Beijing Institute of Technology, Zhengzhou Academy of Intelligent Technology, Zhengzhou450000, China. AD - Beijing Institute of Technology, Chongqing Innovation Center, Chongqing401120, China. FAU - Wang, Bo AU - Wang B AUID- ORCID: 0000-0001-9092-3252 AD - Ministry of Education Key Laboratory of Cluster Science, Beijing Key Laboratory of Intelligent Molecular Materials and High-throughput Manufacturing, Beijing Key Laboratory of Green Hydrogen Energy and Fuel Cells, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing100081, P. R. China. AD - Beijing Institute of Technology, Zhengzhou Academy of Intelligent Technology, Zhengzhou450000, China. AD - Beijing Institute of Technology, Chongqing Innovation Center, Chongqing401120, China. LA - eng GR - NA/Beijing Institute of Technology Research Fund Program for Young Scholars/ GR - 2023YFC3310600/National Basic Research Program of China (973 Program)/ GR - 22275168/National Natural Science Foundation of China (NSFC)/ GR - 22475019/National Natural Science Foundation of China (NSFC)/ GR - 22675026/National Natural Science Foundation of China (NSFC)/ GR - CSTB2023NSCQ-MSX0244/Natural Science Foundation of Chongqing/ GR - CSTB2024NSCQ-QCXMX0057/Chongqing Science and Technology Commission/ GR - CSTB2024TIAD-KPX0060/Chongqing Technology Innovation and Application Development Project/ GR - NA/Chongqing Science and Technology Commission/ GR - NA/Chongqing Technology Innovation and Application Development Project/ GR - NA/National Natural Science Foundation of China (NSFC)/ GR - NA/Natural Science Foundation of Chongqing/ GR - NA/State Key Laboratory of Transient Chemical Effects and Control/ GR - WDYX25614260203/State Key Laboratory of Transient Chemical Effects and Control/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/08/01 00:00 [received] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5453261 [pii] AID - 10.1021/jacs.6c16023 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):46181-46193. doi: 10.1021/jacs.6c16023. PMID- 42954583 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Layer Engineering of Quantum Organic-Inorganic Superlattices by Thermodynamic Design. PG - 45543-45550 LID - 10.1021/jacs.6c11170 [doi] AB - Quantum organic-inorganic superlattices, constructed by vertically stacking two-dimensional inorganic crystals and organic molecular layers, offer a powerful platform for encoding emergent electronic states through an interfacial design. However, the precise layer engineering of such hybrid architectures, particularly the deterministic control over vertical stacking periodicities, has remained elusive because of the absence of a predictive thermodynamic framework governing the staging effect in hybrid systems. Here, we demonstrate that thermodynamic design, based on the quantitative competition between intercalant chemical potential and host elastic deformation energy, provides a principled route to programmable layer engineering of organic-inorganic superlattices. By precisely modulating the chemical potential of the intercalant, we achieve selective access to stage-1 intercalation (alternating 1-layer (1L) molecule/1L-SnSe2) and stage-2 intercalation (alternating 1L-molecule/2L-SnSe2) configurations with high structural fidelity. Atomic-resolution imaging reveals long-range periodic order across both staging phases, while angle-resolved photoemission spectroscopy reveals that the engineered layer sequence directly governs the interfacial charge transfer and electronic band structure evolution. The resulting superlattices exhibit stacking-dependent carrier densities and programmable superconducting transitions, establishing the layer sequence as a synthetic handle for quantum functionality. This work establishes thermodynamic design as a guiding principle for the rational layer engineering of quantum-hybrid solids. CI - (c) 2026 American Chemical Society. FAU - Liu, Yang AU - Liu Y AD - State Key Laboratory of Precision and Intelligent Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Wang, Ziren AU - Wang Z AD - State Key Laboratory of Precision and Intelligent Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Tai, Xiaolin AU - Tai X AD - State Key Laboratory of Precision and Intelligent Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Song, Xuzhe AU - Song X AD - State Key Laboratory of Precision and Intelligent Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Wang, Minghao AU - Wang M AD - State Key Laboratory of Precision and Intelligent Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Guo, Yuqiao AU - Guo Y AD - State Key Laboratory of Precision and Intelligent Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Yang, Ying AU - Yang Y AD - State Key Laboratory of Precision and Intelligent Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Zhang, Yiwei AU - Zhang Y AD - State Key Laboratory of Precision and Intelligent Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Shang, Zhengming AU - Shang Z AD - National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Zhang, Baidu AU - Zhang B AD - CAS Key Laboratory of Mechanical Behavior and Design of Materials, Department of Modern Mechanics, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Hu, Guoliang AU - Hu G AD - State Key Laboratory of Precision and Intelligent Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Liu, Yi AU - Liu Y AD - National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Sun, Zhe AU - Sun Z AD - National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Lv, Haifeng AU - Lv H AUID- ORCID: 0000-0001-9491-6367 AD - State Key Laboratory of Precision and Intelligent Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Lin, Yue AU - Lin Y AUID- ORCID: 0000-0001-5333-511X AD - State Key Laboratory of Precision and Intelligent Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Wu, Xiaojun AU - Wu X AUID- ORCID: 0000-0003-3606-1211 AD - State Key Laboratory of Precision and Intelligent Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Xie, Yi AU - Xie Y AUID- ORCID: 0000-0002-1416-5557 AD - State Key Laboratory of Precision and Intelligent Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Wu, Changzheng AU - Wu C AUID- ORCID: 0000-0002-4416-6358 AD - State Key Laboratory of Precision and Intelligent Chemistry, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. LA - eng GR - 22525013/Youth Science Fund Project/ GR - 12274391/National Natural Science Foundation of China/ GR - 22225301/National Natural Science Foundation of China/ GR - 22303092/National Natural Science Foundation of China/ GR - 22321001/National Natural Science Foundation of China/ GR - 22501267/National Natural Science Foundation of China/ GR - 225B1010/National Natural Science Foundation of China/ GR - 52122212/National Natural Science Foundation of China/ GR - 2025M771006/China Postdoctoral Science Foundation/ GR - GZB20250252/China Postdoctoral Science Foundation/ GR - 2408085J012/Natural Science Foundation of Anhui Province/ GR - 2018500/Youth Innovation Promotion Association of the Chinese Academy of Sciences/ GR - 2022YFA1203600/National Key Research and Development Program of China/ GR - NA/Fundamental Research Funds for the Central Universities/ GR - ZDBS-LY-SLH003/Key Research Program of Frontier Science, Chinese Academy of Sciences/ GR - YD2060002004/USTC Research Funds of the Double First-Class Initiative/ GR - YSBR-070/CAS Project for Young Scientists in Basic Research/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/06/04 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5453164 [pii] AID - 10.1021/jacs.6c11170 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):45543-45550. doi: 10.1021/jacs.6c11170. PMID- 42954582 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Accelerated Zeolite Defect Healing Suppresses Oxygen-Containing Species Trapping for Tandem Propene Epoxidation. PG - 46332-46342 LID - 10.1021/jacs.6c17355 [doi] AB - Selective oxidation of chemical feedstocks with in situ-generated H2O2 offers a sustainable route to high-value oxygenates, yet such tandem catalysis is often fundamentally limited by the trapping of oxygen-containing species, including H2O2-derived intermediates and oxygenated products in zeolite microenvironments, where they undergo nonproductive decomposition and side reactions. Herein, we overcome this limitation by accelerating titanosilicalite hydroxyl defect healing through intensified nucleation and subsequent accelerated crystal growth. The resulting defect-healed microenvironment facilitates the transport of in situ-generated H2O2 between bifunctional active sites while suppressing its nonproductive decomposition to H2O. Moreover, it accelerates product propylene oxide (PO) desorption by weakening hydrogen bond interactions between PO and hydroxyl defects, thereby suppressing ring-opening and overoxidation side reactions. Consequently, the defect-healed Au/TS-1 catalyst exhibits a 1.8-fold enhancement in the PO formation rate and a 2.1-fold increase in Au-based productivity. This defect-healing strategy is broadly applicable to titanosilicates, aluminosilicates, and pure-silica zeolites and can be readily extended to a 10 L scale process, delivering reductions of 21% in production cost and 54% in carbon emissions compared with conventional TS-1 synthesis. Overall, this work establishes a sustainable, scalable, and cost-effective route for zeolite defect healing and provides a general strategy to overcome the long-standing transport-decomposition trade-off in tandem catalysis with in situ-generated H2O2. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Lin, Dong AU - Lin D AUID- ORCID: 0000-0001-5313-8204 AD - State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Qingdao266580, China. AD - Max Planck-Cardiff Centre on the Fundamentals of Heterogeneous Catalysis FUNCAT, Cardiff Catalysis Institute, School of Chemistry, Cardiff University, CardiffCF24 4HQ, U.K. FAU - Zhou, Xin AU - Zhou X AUID- ORCID: 0000-0002-5921-4260 AD - College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao266100, China. FAU - Xu, Yang AU - Xu Y AD - State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Qingdao266580, China. FAU - Lewis, Richard J AU - Lewis RJ AUID- ORCID: 0000-0001-9990-7064 AD - Max Planck-Cardiff Centre on the Fundamentals of Heterogeneous Catalysis FUNCAT, Cardiff Catalysis Institute, School of Chemistry, Cardiff University, CardiffCF24 4HQ, U.K. FAU - Sun, Zongzhuang AU - Sun Z AD - State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Qingdao266580, China. FAU - Yin, Defu AU - Yin D AD - State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Qingdao266580, China. FAU - Wang, Yongxiang AU - Wang Y AD - National Centre for Magnetic Resonance in Wuhan, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan430071, China. FAU - Liu, Zhenyi AU - Liu Z AD - State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Qingdao266580, China. FAU - Shang, Jingyuan AU - Shang J AD - State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Qingdao266580, China. FAU - Ma, Zhe AU - Ma Z AD - State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Qingdao266580, China. FAU - Wang, Qiang AU - Wang Q AUID- ORCID: 0000-0001-7741-3733 AD - National Centre for Magnetic Resonance in Wuhan, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan430071, China. FAU - Deng, Feng AU - Deng F AUID- ORCID: 0000-0002-6461-7152 AD - National Centre for Magnetic Resonance in Wuhan, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan430071, China. FAU - Yang, Chaohe AU - Yang C AUID- ORCID: 0000-0001-6995-9170 AD - State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Qingdao266580, China. FAU - Chen, De AU - Chen D AD - Department of Chemical Engineering, Norwegian University of Science and Technology, Trondheim7491, Norway. FAU - Hutchings, Graham J AU - Hutchings GJ AUID- ORCID: 0000-0001-8885-1560 AD - Max Planck-Cardiff Centre on the Fundamentals of Heterogeneous Catalysis FUNCAT, Cardiff Catalysis Institute, School of Chemistry, Cardiff University, CardiffCF24 4HQ, U.K. FAU - Feng, Xiang AU - Feng X AUID- ORCID: 0000-0001-7299-5690 AD - State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Qingdao266580, China. LA - eng GR - EP/Z002400/1/Engineering and Physical Sciences Research Council/ GR - NA/Cardiff University/ GR - 22322814/National Natural Science Foundation of China/ GR - U25A20600/National Natural Science Foundation of China/ GR - NA/Max-Planck-Gesellschaft/ GR - ZR2024JQ009/Natural Science Foundation of Shandong Province/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/08/18 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5453156 [pii] AID - 10.1021/jacs.6c17355 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):46332-46342. doi: 10.1021/jacs.6c17355. PMID- 42954575 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Cu-N3 Single-Atom Catalysts Enable High-Turnover C-N and C-O Cross-Coupling of Aryl Electrophiles. PG - 45926-45934 LID - 10.1021/jacs.6c14402 [doi] AB - Transition-metal-catalyzed C-N cross-coupling is fundamental to the synthesis of pharmaceuticals, agrochemicals, and functional materials. However, many copper-based catalytic systems rely on exogenous molecular ligands to establish and tune the coordination environment around copper centers, which can increase catalyst-design complexity and complicate catalyst recovery and reuse. We report a copper single-atom catalyst (Cu-N3/OMC) that enables ligand-free C-N and C-O cross-coupling of aryl electrophiles, with isolated Cu sites embedded in a nitrogen-doped carbon framework that provides an intrinsic coordination environment. This system enables ligand-free copper-catalyzed C-N coupling of synthetically relevant aryl bromides and further extends the reaction to less-activated aryl chlorides. A broad range of substituted aryl and heteroaryl bromides horizontal line including electron-rich, electron-deficient, ortho-substituted, and fused substrates horizontal line undergo efficient coupling with diverse amines and alcohols, affording the corresponding products in moderate to excellent yields (up to 96%). Selected aryl chlorides are also compatible under KI-promoted conditions, albeit with reduced efficiency in some cases. The methodology is applicable to late-stage functionalization of complex pharmaceutical molecules and can be conducted on a preparative scale (5-100 mmol), affording yields of 90-95% and TONs of up to 84,000 under air. TON values were calculated based on the total Cu loading. More broadly, this work suggests that support-engineered coordination environments can influence the reactivity of isolated Cu sites, providing a strategy for ligand-free heteroatom cross-coupling. CI - (c) 2026 American Chemical Society. FAU - Ge, Liang AU - Ge L AD - School of Chemistry & Chemical Engineering, Anhui University of Technology, Ma'anshan, Anhui243002, P. R. China. FAU - Chen, Jianguo AU - Chen J AD - School of Chemistry & Chemical Engineering, Anhui University of Technology, Ma'anshan, Anhui243002, P. R. China. FAU - Song, Nan AU - Song N AD - State Key Laboratory of Natural Product Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou730000, P.R. China. FAU - Jiang, Jiarui AU - Jiang J AD - State Key Laboratory of Natural Product Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou730000, P.R. China. FAU - Xue, Zaibin AU - Xue Z AD - School of Chemistry & Chemical Engineering, Anhui University of Technology, Ma'anshan, Anhui243002, P. R. China. FAU - Liu, Mingkai AU - Liu M AUID- ORCID: 0000-0001-9060-848X AD - School of Chemistry & Chemical Engineering, Anhui University of Technology, Ma'anshan, Anhui243002, P. R. China. FAU - Tang, Yu AU - Tang Y AUID- ORCID: 0000-0003-3933-043X AD - State Key Laboratory of Natural Product Chemistry, Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou730000, P.R. China. FAU - Zeng, Jie AU - Zeng J AUID- ORCID: 0000-0002-8812-0298 AD - School of Chemistry & Chemical Engineering, Anhui University of Technology, Ma'anshan, Anhui243002, P. R. China. AD - Hefei National Research Center for Physical Sciences at the Microscale, Key Laboratory of Strongly-Coupled Quantum Matter Physics of Chinese Academy of Sciences, Key Laboratory of Surface and Interface Chemistry and Energy Catalysis of Anhui Higher Education Institutes, Department of Chemical Physics, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. AD - Deep Space Exploration Laboratory, Hefei, Anhui230088, P. R. China. LA - eng GR - 2308085J16/Science Fund for Distinguished Young Scholars of Anhui Province/ GR - YSBR051/Chinese Academy of Sciences/ GR - 2508085ZD020/Natural Science Foundation of Anhui Province/ GR - 0070/2023/AFJ/Fundo para o Desenvolvimento das Ci?ncias e da Tecnologia/ GR - KY9990002504/Xinjiang Normal University/ GR - KY9990002504/University of Science and Technology of China/ GR - LS2060000002/University of Science and Technology of China/ GR - 2024SKLA011/State Key Laboratory of Catalysis/ GR - NA/Fundamental Research Funds for the Central Universities/ GR - 2021YFA1500500/National Basic Research Program of China (973 Program)/ GR - 2024YFB4105700/National Basic Research Program of China (973 Program)/ GR - 22221003/National Natural Science Foundation of China (NSFC)/ GR - 22250007/National Natural Science Foundation of China (NSFC)/ GR - 22361162655/National Natural Science Foundation of China (NSFC)/ GR - 22401197/National Natural Science Foundation of China (NSFC)/ GR - 22475003/National Natural Science Foundation of China (NSFC)/ GR - 22525021/National Natural Science Foundation of China (NSFC)/ GR - BT2025014/Jiangsu Science and Technology Department/ GR - NA/New Cornerstone Science Foundation/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/07/13 00:00 [received] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5452884 [pii] AID - 10.1021/jacs.6c14402 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):45926-45934. doi: 10.1021/jacs.6c14402. PMID- 42954570 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Surface-Layer Pore Aperture Engineering in a Metal-Organic Framework for High-Capacity CO2/C2H2 Molecular Sieving. PG - 45962-45970 LID - 10.1021/jacs.6c14696 [doi] AB - Molecular sieving in metal-organic frameworks (MOFs) is commonly achieved by narrowing the entire pore network, which enhances size exclusion but often sacrifices adsorption capacity. Here we report surface-layer pore aperture engineering as a spatially decoupled strategy for high-capacity CO2/C2H2 molecular sieving. Through surface-confined coordination modification of a copper-based MOF, the external pore apertures were selectively regulated while the internal adsorption space was largely preserved. Microscopy, elemental mapping, depth-resolved XPS, and X-ray absorption spectroscopy support the formation of a surface-confined functional shell at the pore aperture. The modified framework converts a nonselective, high-capacity adsorbent into a CO2/C2H2 molecular sieve. BUT-169-NH2-TRZ retains a high CO2 uptake of 90.3 cm3 g-1 at 298 K and 1 bar while suppressing C2H2 adsorption to 2.7 cm3 g-1, representing a new benchmark for CO2/C2H2 sieving separation. Further optimization with hexylamine affords faster CO2 transport and dynamic CO2/C2H2 separation, delivering a high productivity of 1.13 mol kg-1 for C2H2 (purity > 99.99%) over repeated breakthrough cycles. This work establishes surface-layer pore aperture engineering as a route to overcome the conventional selectivity-capacity trade-off in molecular sieving separations. CI - (c) 2026 American Chemical Society. FAU - Li, Muzi AU - Li M AD - State Key Laboratory of Materials Low-Carbon Recycling, Department of Chemical Engineering, College of Materials Science & Engineering, Beijing University of Technology, Beijing100124, China. FAU - Wang, Yingjie AU - Wang Y AD - State Key Laboratory of Materials Low-Carbon Recycling, Department of Chemical Engineering, College of Materials Science & Engineering, Beijing University of Technology, Beijing100124, China. FAU - Zhang, Xin AU - Zhang X AUID- ORCID: 0000-0001-9318-8839 AD - State Key Laboratory of Materials Low-Carbon Recycling, Department of Chemical Engineering, College of Materials Science & Engineering, Beijing University of Technology, Beijing100124, China. FAU - Bai, Xuefeng AU - Bai X AD - State Key Laboratory of Materials Low-Carbon Recycling, Department of Chemical Engineering, College of Materials Science & Engineering, Beijing University of Technology, Beijing100124, China. FAU - Liu, Lu AU - Liu L AD - State Key Laboratory of Materials Low-Carbon Recycling, Department of Chemical Engineering, College of Materials Science & Engineering, Beijing University of Technology, Beijing100124, China. FAU - Li, Jianyun AU - Li J AD - State Key Laboratory of Materials Low-Carbon Recycling, Department of Chemical Engineering, College of Materials Science & Engineering, Beijing University of Technology, Beijing100124, China. FAU - Xie, Lin-Hua AU - Xie LH AUID- ORCID: 0000-0003-0017-3887 AD - State Key Laboratory of Materials Low-Carbon Recycling, Department of Chemical Engineering, College of Materials Science & Engineering, Beijing University of Technology, Beijing100124, China. FAU - Li, Jian-Rong AU - Li JR AUID- ORCID: 0000-0002-8101-8493 AD - State Key Laboratory of Materials Low-Carbon Recycling, Department of Chemical Engineering, College of Materials Science & Engineering, Beijing University of Technology, Beijing100124, China. LA - eng GR - 22225803/National Natural Science Foundation of China (NSFC)/ GR - 22278011/National Natural Science Foundation of China (NSFC)/ GR - 22522801/National Natural Science Foundation of China (NSFC)/ GR - JWZQ20240102008/Beijing Outstanding Talent Training Foundation/ GR - NA/Beijing Municipal Natural Science Foundation/ GR - NA/Beijing Outstanding Talent Training Foundation/ GR - U25A20621/National Natural Science Foundation of China (NSFC)/ GR - Z230023/Beijing Municipal Natural Science Foundation/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/07/16 00:00 [received] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5452473 [pii] AID - 10.1021/jacs.6c14696 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):45962-45970. doi: 10.1021/jacs.6c14696. PMID- 42954564 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Dynamics and Structure of Room Temperature Ionic Liquids: Ultrafast IR Experiments and MD Simulations of the Ion Cage Model. PG - 45551-45564 LID - 10.1021/jacs.6c11518 [doi] AB - Dynamics in room-temperature ionic liquids (ILs) and the solvation of charged and neutral molecules are important to applications of ILs in battery technology, organic synthesis, and many other areas. Using ultrafast IR spectroscopy, pulse-field-gradient (pfg) NMR, and MD simulations, we investigated three ionic liquids that share the same cation but differ in their anions. IR polarization-selective pump-probe and 2D IR experiments were performed on a negatively charged probe, thiocyanate, to investigate IL dynamics. It was found that thiocyanate is an excellent surrogate for the IL anions. We employed a neutral probe, methylthiocyanate, to determine how a neutral solute's dynamics and interactions differ from those of the RTIL ions. The pfg-NMR experiments, performed on all liquids and species, measured the translational diffusion constants. Extensive molecular dynamics simulations were performed to understand the experimental observables within the IL cage model, in which cations surround an anion (anion cage), and anions surround a cation (cation cage) for all ionic species, including the ionic probe. The neutral probe is surrounded by a combination of cations and anions (neutral probe cage). The cage model shows that the dynamical properties, including translational diffusion, orientational diffusion, and viscosity, are determined by the cage escape time, te, i.e., the time for an ion to leave the group of ions surrounding an ion of the opposite charge or the neutral probe. It is observed that te is correlated with the slowest component of the cage structural fluctuations measured by 2D IR. CI - (c) 2026 American Chemical Society. FAU - Leibfried, Laura C AU - Leibfried LC AD - Department of Chemistry, Stanford University, Stanford, California94305, United States. FAU - Sha, Maolin AU - Sha M AD - Department of Physics and Materials Engineering, Hefei Normal University, Hefei230061, China. FAU - Fayer, Michael D AU - Fayer MD AUID- ORCID: 0000-0002-0021-1815 AD - Department of Chemistry, Stanford University, Stanford, California94305, United States. LA - eng GR - FA9550-24-1-0145/Air Force Office of Scientific Research/ GR - 2024AH040215/Natural Science?Foundation of the Anhui Higher Education Institutions of China/ GR - ECCS-2026822/Stanford Nano Shared Facilities (SNSF), National Science Foundation/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/06/04 00:00 [received] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5452268 [pii] AID - 10.1021/jacs.6c11518 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):45551-45564. doi: 10.1021/jacs.6c11518. PMID- 42954562 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Regioselective and Stereodivergent C-H Functionalization of Internal Alkenes. PG - 45330-45336 LID - 10.1021/jacs.6c17366 [doi] AB - Herein, we introduce a new strategy for the regioselective and stereodivergent C-H functionalization of internal alkenes. We discovered that the regioselectivity of internal alkene thianthrenation is exceptionally electronically sensitive and can be controlled by native allylic and homoallylic functional groups. We also found that both alkenyl thianthrenium stereoisomers can be selectively accessed by using different workup conditions. While thianthrenation is typically stereoretentive, the addition of Cs2CO3 in the workup induces isomerization through reversible conjugate addition. We show that both stereoisomers of the alkenyl linchpins can be readily engaged in stereoretentive cross coupling reactions. This selective thianthrenation-coupling sequence introduces a generalizable platform for stereodefined trisubstituted alkene synthesis. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Ryutov, Elizabeth A AU - Ryutov EA AD - Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin53706, United States. FAU - Verardi, Peter J AU - Verardi PJ AD - Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin53706, United States. FAU - Dang, Song N AU - Dang SN AD - Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin53706, United States. FAU - Targos, Karina AU - Targos K AD - Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin53706, United States. FAU - Garman, Leah C AU - Garman LC AD - Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin53706, United States. FAU - Wickens, Zachary K AU - Wickens ZK AUID- ORCID: 0000-0002-5733-5288 AD - Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin53706, United States. LA - eng GR - 1S10OD020022-1/NIH Office of the Director/ GR - S10OD012245/NIH Office of the Director/ GR - R01 GM149674-01/GM/NIGMS NIH HHS/United States GR - 1048642/Division of Chemistry/ GR - 2137424/National Science Foundation Graduate Research Fellowship Program/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/08/20 00:00 [received] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/09/24 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5452197 [pii] AID - 10.1021/jacs.6c17366 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):45330-45336. doi: 10.1021/jacs.6c17366. PMID- 42954556 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Unilateral Functionalization of Covalent Organic Frameworks: Local Electronic Modulation and Morphology-Enabled Mass Transport for Electrocatalytic Hydrogen Peroxide Production. PG - 46298-46309 LID - 10.1021/jacs.6c17269 [doi] AB - Achieving high selectivity and activity in the two-electron oxygen reduction reaction (2e- ORR) pathway critically depends on the synergistic modulation of a catalyst's electronic structure and mass diffusion channels. This work proposes an asymmetric modulation strategy via unilateral functionalization, in which -CH3, -CN, -SO3H, and -NO2 moieties are incorporated into a series of covalent organic frameworks (COFs), systematically unraveling the multilevel regulatory mechanisms exerted by functional groups from molecular to macroscopic morphological scales. The research demonstrates that introducing strong electron-withdrawing -NO2 groups can effectively enhance charge transfer at adjacent carbon active sites, reduce the formation barrier of OOH* intermediates, and induce efficient 2e- ORR activity. Concurrently, the polarity and steric effects of functional groups induce structural evolution in COFs from spherical, rod-like to spiderweb-like architectures, wherein the interconnected network of COF-NO2 substantially enhances the diffusion efficiency of reactants and products. This synergy between electronic structure and macroscopic morphology enables COF-NO2 to achieve 94% H2O2 selectivity and a high production rate of 10.5 mol gcat-1 h-1 with sustained stability. This work not only provides outstanding catalysts for efficient electrochemical H2O2 synthesis but also clarifies functional group-induced electronic and morphological modulation, offering a novel paradigm for the rational design of porous catalytic materials. CI - (c) 2026 American Chemical Society. FAU - Xi, Xueqing AU - Xi X AD - State Key Laboratory of Bio-Fibers and Eco-Textiles, Institute of Marine Biobased Materials, College of Materials Science and Engineering, Qingdao University, Qingdao266071, P. R. China. FAU - Wang, Chao AU - Wang C AUID- ORCID: 0000-0002-6230-817X AD - State Key Laboratory of Bio-Fibers and Eco-Textiles, Institute of Marine Biobased Materials, College of Materials Science and Engineering, Qingdao University, Qingdao266071, P. R. China. FAU - Xing, Yali AU - Xing Y AD - State Key Laboratory of Bio-Fibers and Eco-Textiles, Institute of Marine Biobased Materials, College of Materials Science and Engineering, Qingdao University, Qingdao266071, P. R. China. FAU - Li, Lili AU - Li L AD - State Key Laboratory of Bio-Fibers and Eco-Textiles, Institute of Marine Biobased Materials, College of Materials Science and Engineering, Qingdao University, Qingdao266071, P. R. China. FAU - Li, Daohao AU - Li D AUID- ORCID: 0000-0003-2040-5227 AD - State Key Laboratory of Bio-Fibers and Eco-Textiles, Institute of Marine Biobased Materials, College of Materials Science and Engineering, Qingdao University, Qingdao266071, P. R. China. FAU - Dong, Ziyan AU - Dong Z AD - State Key Laboratory of Bio-Fibers and Eco-Textiles, Institute of Marine Biobased Materials, College of Materials Science and Engineering, Qingdao University, Qingdao266071, P. R. China. FAU - Chen, Yunqing AU - Chen Y AD - Department of Pathology, The Affiliated Hospital of Qingdao University, Qingdao266003, P. R. China. FAU - Long, Xiaojing AU - Long X AUID- ORCID: 0000-0002-9899-1820 AD - State Key Laboratory of Bio-Fibers and Eco-Textiles, Institute of Marine Biobased Materials, College of Materials Science and Engineering, Qingdao University, Qingdao266071, P. R. China. LA - eng GR - 22375111/National Natural Science Foundation of China/ GR - 52302272/National Natural Science Foundation of China/ GR - ZR2024JQ037/Natural Science Foundation of Shandong Province/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/08/18 00:00 [received] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5451734 [pii] AID - 10.1021/jacs.6c17269 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):46298-46309. doi: 10.1021/jacs.6c17269. PMID- 42954553 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Functionalized Olefin-Linked Covalent Organic Frameworks for Selective Mercury Capture and Recovery from Vinyl Chloride-Rich Gas Streams. PG - 45775-45787 LID - 10.1021/jacs.6c13542 [doi] AB - The calcium carbide-derived acetylene hydrochlorination route for vinyl chloride monomer (VCM, C2H3Cl) production remains a major source of mercury consumption and emission owing to its reliance on HgCl2/activated carbon catalysts. Selective removal of elemental mercury (Hg0) from crude VCM streams is intrinsically challenging because Hg0 must be captured from C2H3Cl-rich and HCl-containing atmospheres without coadsorbing valuable C2H3Cl. Here, we report a pore-engineered covalent organic framework (COF) platform in which olefin-linked sp2-carbon-conjugated frameworks suppress nonspecific C2H3Cl adsorption, while Cu-coordinated 2-(methylthio)ethoxy side chains create well-defined Cu-O2S2 pockets for selective Hg0 capture. These structural features afford an ideal adsorbed solution theory-predicted Hg0/C2H3Cl selectivity exceeding 20,000. Notably, dynamic breakthrough experiments under simulated industrial conditions containing 5 vol % HCl further revealed a Hg0 breakthrough time of over 10,000 min g-1 in C2H3Cl-rich streams, with a separation factor of approximately 52, demonstrating exceptional resistance to acid-induced deactivation. Treatment of the spent adsorbent with Na2S solution enables regeneration over 30 consecutive cycles and converts captured mercury into chemically stable HgS. This work establishes a molecularly precise removal-recovery platform for elemental mercury from chemically complex industrial gas streams, highlighting the power of programmed pore microenvironments to discriminate trace toxic species from abundant value-added molecules under chemically aggressive conditions. CI - (c) 2026 American Chemical Society. FAU - Wang, Mingming AU - Wang M AD - State Key Laboratory of Green Papermaking and Resource Recycling, School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai200240, China. FAU - Xu, Haomiao AU - Xu H AUID- ORCID: 0000-0003-3736-4548 AD - State Key Laboratory of Green Papermaking and Resource Recycling, School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai200240, China. AD - Yunnan Dali Research Institute of Shanghai Jiao Tong University, Dali67100, China. FAU - Fan, Yurui AU - Fan Y AUID- ORCID: 0000-0002-3536-1880 AD - State Key Laboratory of Green Papermaking and Resource Recycling, School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai200240, China. FAU - Shi, Chaobin AU - Shi C AD - State Key Laboratory of Green Papermaking and Resource Recycling, School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai200240, China. FAU - Lei, Jun AU - Lei J AD - State Key Laboratory of Green Papermaking and Resource Recycling, School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai200240, China. FAU - Lai, Jiawei AU - Lai J AD - State Key Laboratory of Green Papermaking and Resource Recycling, School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai200240, China. FAU - Li, Weiwei AU - Li W AD - School of Chemistry and Chemical Engineering and State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai200240, China. FAU - Hong, Qinyuan AU - Hong Q AUID- ORCID: 0000-0002-0733-7402 AD - State Key Laboratory of Green Papermaking and Resource Recycling, School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai200240, China. FAU - Huang, Wenjun AU - Huang W AUID- ORCID: 0000-0001-8887-309X AD - State Key Laboratory of Green Papermaking and Resource Recycling, School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai200240, China. FAU - Qu, Zan AU - Qu Z AUID- ORCID: 0000-0003-2193-2715 AD - School of Chemical Engineering, Xinjiang University, Urumqi, Xinjiang830017, China. FAU - Cui, Yong AU - Cui Y AUID- ORCID: 0000-0003-1977-0470 AD - School of Chemistry and Chemical Engineering and State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai200240, China. FAU - Han, Xing AU - Han X AUID- ORCID: 0000-0002-7921-8197 AD - State Key Laboratory of Green Papermaking and Resource Recycling, School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai200240, China. AD - Yunnan Dali Research Institute of Shanghai Jiao Tong University, Dali67100, China. FAU - Yan, Naiqiang AU - Yan N AUID- ORCID: 0000-0002-7821-1997 AD - State Key Laboratory of Green Papermaking and Resource Recycling, School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai200240, China. AD - Yunnan Dali Research Institute of Shanghai Jiao Tong University, Dali67100, China. AD - Shanghai Institute of Pollution Control and Ecological Security, Shanghai200092, China. LA - eng GR - 25ZR1402208/Natural Science Foundation of Shanghai Municipality/ GR - 22436004/National Natural Science Foundation of China/ GR - 22476125/National Natural Science Foundation of China/ GR - 2025M781204/China Postdoctoral Science Foundation/ GR - 22522608/Excellent Young Scientists Fund/ GR - 2024YFB4105100/National Key Research and Development Program of China/ GR - 2024YFC3907903/National Key Research and Development Program of China/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/07/01 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5451645 [pii] AID - 10.1021/jacs.6c13542 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):45775-45787. doi: 10.1021/jacs.6c13542. PMID- 42954551 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Solar Biorefinery: Stoichiometric Coproduction of Gluconic and Glucaric Acids and Impurity-Free Hydrogen from Lignocellulosic Biomass and Water. PG - 46032-46044 LID - 10.1021/jacs.6c15106 [doi] AB - Solar biorefinery serves as a game changer in the sustainable production of transportation fuels and value-added chemicals by capitalizing on sunlight, lignocellulosic biomass, and water. Central to this endeavor is the modulation of highly reactive, nonselective hydroxyl radicals to prevent undesired side reactions. Here, surface-hydroxylated nickel oxyphosphide works synergistically with one-dimensional gallium nitride, enabling the selective reforming of lignocellulose in water toward glucose-derived organic acids (GOAs: gluconic and glucaric acids) and H2 at a nearly stoichiometric ratio: (C6H12O6)n+ H2O + sunlight --> GOAs + H2. The GaN@NiOP-OH system achieves an H2 evolution rate of 2.55 mol g-1 h-1 with 100% gas-phase selectivity under simulated concentrated sunlight at 3.5 W cm-2. GOAs are simultaneously produced at a total rate of 2.23 mol g-1 h-1, corresponding to a near-stoichiometric GOAs/H2 ratio of 0.97 with nearly 100% atom utilization efficiency. This platform is broadly applicable to sunlight-driven reforming of cellulose, straw, and camphorwood. In situ spectroscopy, isotope labeling, and density functional theory calculations demonstrate that NiOP-OH cooperates with GaN to modulate in situ-generated hydroxyl radical species originating from water oxidation by photogenerated holes. These radicals are confined on the catalytic surface with their nonselective reactivity finely tuned, enabling selective conversion of glucose with H2O into GOAs and H2 via a dynamic hydroxyl exchange mechanism while suppressing side and mineralization reactions. Outdoor testing and techno-economic analysis confirm the practical viability of this proof-of-concept solar biorefinery for coproducing high-value chemicals and green H2 using only sunlight, lignocellulose, and water. CI - (c) 2026 American Chemical Society. FAU - Wang, Ding AU - Wang D AD - Key Laboratory for Power Machinery and Engineering of Ministry of Education, Research Center for Renewable Synthetic Fuel, School of Mechanical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai200240, China. AD - Shanghai Key Laboratory of the Preparation and Utilization of Green Hydrogen-based Fuels, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai200240, P.R. China. FAU - Wu, Zhaosong AU - Wu Z AD - Key Laboratory for Power Machinery and Engineering of Ministry of Education, Research Center for Renewable Synthetic Fuel, School of Mechanical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai200240, China. AD - Shanghai Key Laboratory of the Preparation and Utilization of Green Hydrogen-based Fuels, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai200240, P.R. China. FAU - Zheng, Xiantong AU - Zheng X AD - College of Instrument Science and Optoelectronics Engineering, Beijing Information Science and Technology University, Beijing100096, China. FAU - Huang, Zijian AU - Huang Z AD - China-UK Low Carbon College, Shanghai Jiao Tong University, No.3 Yinlian Road, Lingang, Shanghai201306, China. FAU - Yao, Lin AU - Yao L AUID- ORCID: 0009-0006-6283-2146 AD - China-UK Low Carbon College, Shanghai Jiao Tong University, No.3 Yinlian Road, Lingang, Shanghai201306, China. FAU - Wang, Ping AU - Wang P AUID- ORCID: 0000-0002-4716-5457 AD - State Key Laboratory of Artificial Microstructure and Mesoscopic Physics, School of Physics, Nano-Optoelectronics Frontier Center of Ministry of Education (NFC-MOE), Peking University, Beijing100871, China. FAU - Wang, Xinqiang AU - Wang X AD - State Key Laboratory of Artificial Microstructure and Mesoscopic Physics, School of Physics, Nano-Optoelectronics Frontier Center of Ministry of Education (NFC-MOE), Peking University, Beijing100871, China. AD - Peking University Yangtze Delta Institute of Optoelectronics, Nantong, Jiangsu226010, China. AD - Collaborative Innovation Center of Quantum Matter, School of Physics, Peking University, Beijing100871, China. FAU - Liu, Jiaming AU - Liu J AD - Key Laboratory for Power Machinery and Engineering of Ministry of Education, Research Center for Renewable Synthetic Fuel, School of Mechanical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai200240, China. AD - Shanghai Key Laboratory of the Preparation and Utilization of Green Hydrogen-based Fuels, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai200240, P.R. China. FAU - Nasir, Muhammad Salman AU - Nasir MS AD - State Key Laboratory of Engines, School of Mechanical Engineering, Tianjin University, Tianjin300072, China. FAU - Qiu, Liang AU - Qiu L AD - Key Laboratory for Power Machinery and Engineering of Ministry of Education, Research Center for Renewable Synthetic Fuel, School of Mechanical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai200240, China. AD - Shanghai Key Laboratory of the Preparation and Utilization of Green Hydrogen-based Fuels, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai200240, P.R. China. FAU - Li, Yixin AU - Li Y AD - Key Laboratory for Power Machinery and Engineering of Ministry of Education, Research Center for Renewable Synthetic Fuel, School of Mechanical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai200240, China. AD - Shanghai Key Laboratory of the Preparation and Utilization of Green Hydrogen-based Fuels, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai200240, P.R. China. FAU - Li, Yumeng AU - Li Y AD - China-UK Low Carbon College, Shanghai Jiao Tong University, No.3 Yinlian Road, Lingang, Shanghai201306, China. FAU - Huang, Zhen AU - Huang Z AD - Key Laboratory for Power Machinery and Engineering of Ministry of Education, Research Center for Renewable Synthetic Fuel, School of Mechanical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai200240, China. AD - Shanghai Key Laboratory of the Preparation and Utilization of Green Hydrogen-based Fuels, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai200240, P.R. China. FAU - Han, Buxing AU - Han B AUID- ORCID: 0000-0003-0440-809X AD - Beijing National Laboratory for Molecular Sciences, CAS Key Laboratory of Colloid and Interface and Thermodynamics, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing100190, China. FAU - Zhou, Baowen AU - Zhou B AUID- ORCID: 0000-0003-2476-0322 AD - Key Laboratory for Power Machinery and Engineering of Ministry of Education, Research Center for Renewable Synthetic Fuel, School of Mechanical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai200240, China. AD - Shanghai Key Laboratory of the Preparation and Utilization of Green Hydrogen-based Fuels, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai200240, P.R. China. LA - eng GR - 22121002/National Natural Science Foundation of China/ GR - 22293015/National Natural Science Foundation of China/ GR - 22579110/National Natural Science Foundation of China/ GR - 62321004/National Natural Science Foundation of China/ GR - 21TQ1400211/Shanghai Jiao Tong University/ GR - 2023YFB4004900/National Key Research and Development Program of China/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/07/21 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5451634 [pii] AID - 10.1021/jacs.6c15106 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):46032-46044. doi: 10.1021/jacs.6c15106. PMID- 42954547 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Tuning Second-Sphere Amine Basicity to Modulate Hydride Intermediates and Selectivity in Molecular Manganese Bipyridine CO2 Electroreduction. PG - 45763-45774 LID - 10.1021/jacs.6c13504 [doi] AB - Manganese bipyridine tricarbonyl complexes represent a prominent class of molecular electrocatalysts for efficient and selective CO2-to-CO conversion. Recent efforts have sought to redirect product selectivity toward HCOOH by incorporating second-sphere amine proton relays. However, how the physicochemical properties of second-sphere amines govern catalytic activity and selectivity remains insufficiently understood. In this work, we report a new manganese bipyridine complex bearing 2-(pyrrolidin-1-ylmethyl)phenyl substituents at the 6,6'-positions and systematically evaluate its performance against the diethylamine- and morpholine-functionalized analogs to elucidate the role of second-sphere amine basicity on CO2 reduction. The pyrrolidine-functionalized catalyst achieves near-unity selectivity for HCOOH in acetonitrile with a Faradaic efficiency of 92%, outperforming the corresponding diethylamine (71%) and morpholine (23%) derivatives. This complex also displays outstanding catalytic activity with maximum turnover frequencies of 2300 s-1 and up to 70,000 s-1 under low- and high-overpotential conditions, respectively, ranking among the most active manganese bipyridine catalysts for CO2-to-HCOOH conversion. Mechanistic investigations reveal that the pyrrolidine groups function as proton relays, facilitating proton transfer to the manganese center and promoting formation of the key Mn-hydride intermediate for CO2-to-HCOOH conversion. Density functional theory calculations further demonstrate that the stronger basicity of the pyrrolidine functionality lowers the energy barriers for amine protonation and subsequent intramolecular proton transfer by 3-6 kcal mol-1 relative to the less basic diethylamine and morpholine analogs. Overall, this work establishes second-sphere amine basicity as a key molecular parameter for tuning both activity and product selectivity in the development of molecular CO2 reduction catalysts. CI - (c) 2026 American Chemical Society. FAU - Hong, Wanwan AU - Hong W AUID- ORCID: 0000-0003-1615-0137 AD - Zhongyuan Critical Metals Laboratory, Zhengzhou University, Zhengzhou, Henan450001, China. AD - Novo Nordisk Foundation (NNF) CO2 Research Center, Department of Chemistry, Aarhus University, Gustav Wieds Vej 10C, 8000Aarhus C, Denmark. FAU - Ye, Ke AU - Ye K AD - Division of Theoretical Chemistry and Biology, KTH Royal Institute of Technology, Stockholm10691, Sweden. FAU - Jakobsen, Joakim B AU - Jakobsen JB AUID- ORCID: 0000-0003-4369-3858 AD - Novo Nordisk Foundation (NNF) CO2 Research Center, Department of Chemistry, Aarhus University, Gustav Wieds Vej 10C, 8000Aarhus C, Denmark. FAU - Li, Lin AU - Li L AD - Novo Nordisk Foundation (NNF) CO2 Research Center, Department of Chemistry, Aarhus University, Gustav Wieds Vej 10C, 8000Aarhus C, Denmark. FAU - Del Carmen-Rodriguez, Clara AU - Del Carmen-Rodriguez C AD - GIR MIOMET/IU CINQUIMA/Quimica Inorganica, Facultad de Ciencias, Universidad de Valladolid, ValladolidE-47011, Spain. FAU - Skrydstrup, Troels AU - Skrydstrup T AUID- ORCID: 0000-0001-8090-5050 AD - Novo Nordisk Foundation (NNF) CO2 Research Center, Department of Chemistry, Aarhus University, Gustav Wieds Vej 10C, 8000Aarhus C, Denmark. FAU - Pedersen, Steen U AU - Pedersen SU AUID- ORCID: 0000-0002-8919-2576 AD - Novo Nordisk Foundation (NNF) CO2 Research Center, Department of Chemistry, Aarhus University, Gustav Wieds Vej 10C, 8000Aarhus C, Denmark. FAU - Ahlquist, Marten S G AU - Ahlquist MSG AUID- ORCID: 0000-0002-1553-4027 AD - Division of Theoretical Chemistry and Biology, KTH Royal Institute of Technology, Stockholm10691, Sweden. FAU - Daasbjerg, Kim AU - Daasbjerg K AUID- ORCID: 0000-0003-0212-8190 AD - Novo Nordisk Foundation (NNF) CO2 Research Center, Department of Chemistry, Aarhus University, Gustav Wieds Vej 10C, 8000Aarhus C, Denmark. LA - eng GR - NNF21SA0072700/Novo Nordisk Foundation CO2 Research Center, Aarhus University/ GR - DNRF118/Danmarks Grundforskningsfond/ GR - DNRF93/Danmarks Grundforskningsfond/ GR - 202006370038/China Scholarship Council/ GR - 202106370028/China Scholarship Council/ GR - 2018-05973/National Academic Infrastructure for Supercomputing in Sweden, National Supercomputer Center/ GR - 2022-06725/Swedish Research Council (VR)/ GR - 85378/NordCO2/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/07/01 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5451569 [pii] AID - 10.1021/jacs.6c13504 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):45763-45774. doi: 10.1021/jacs.6c13504. PMID- 42954541 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Catalytic Asymmetric 1,6-Diene Cyclization via Designed Carbocationic 5- and 6-exo Pathways. PG - 45406-45418 LID - 10.1021/jacs.6c08713 [doi] AB - Catalytic asymmetric electrophilic alkene functionalization provides a powerful strategy for the construction of complex chiral molecules. Despite significant progress in this field, the asymmetric functionalization of alkenes promoted by an electrophilic carbon species via an exo-cyclization mode remains a formidable challenge. Herein, we report a solution to this challenge: alkenes can undergo designed 5-exo- and 6-exo-carbocationic cyclizations to generate optically pure, complex chiral compounds with high enantio- and diastereoselectivities. In this approach, for 1,6-dienes bearing bulky sulfonamide or quaternary carbon linkers, one double bond of the substrate interacts with an external electrophile, such as a trifluoromethylthio, arylthio, alkylthio, or chlorine species, generating an electrophilic carbon species that promotes the transformation of 1,6-dienes under chiral indane-based chalcogenide catalysis. Notably, the reaction can be terminated by intramolecular nucleophiles via cis-addition or can be captured by various intermolecular nucleophiles. A diverse array of optically pure polycyclic and monocyclic compounds can be obtained, including 5-5-6 tricyclic N-heterocycles and pentasubstituted chiral cyclohexanes. The same strategy can also be efficiently applied to 6-endo cyclizations, leading to the formation of 6-6-6 tricyclic and monocyclic N-heterocycles. The resulting products can be readily converted to various other valuable compounds, further demonstrating the utility of this methodology. Density functional theory (DFT) calculations were performed to rationalize the observed selectivities, revealing that the preferential transition-state conformations lower the activation barriers for the desired cyclization pathways. This work offers a versatile platform for the synthesis of enantioenriched chiral scaffolds prevalent in bioactive molecules and pharmaceuticals, significantly expanding the scope of polyene cyclization chemistry. CI - (c) 2026 American Chemical Society. FAU - Liu, Xiang AU - Liu X AUID- ORCID: 0000-0001-6973-3809 AD - MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, and Guangdong Key Laboratory of Chiral Molecule and Drug Discovery, Sun Yat-Sen University, Guangzhou510006, China. AD - School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Zhongshan528458, China. FAU - Zhao, Tianteng AU - Zhao T AD - MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, and Guangdong Key Laboratory of Chiral Molecule and Drug Discovery, Sun Yat-Sen University, Guangzhou510006, China. FAU - Li, Junjie AU - Li J AD - State Key Laboratory of Elemento-Organic Chemistry and Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Frontiers Science Center for New Organic Matter, Nankai University, Tianjin300071, China. AD - Haihe Laboratory of Sustainable Chemical Transformations, Tianjin300192, China. FAU - Cao, Qingxiang AU - Cao Q AD - MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, and Guangdong Key Laboratory of Chiral Molecule and Drug Discovery, Sun Yat-Sen University, Guangzhou510006, China. FAU - Zhong, Fuming AU - Zhong F AD - MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, and Guangdong Key Laboratory of Chiral Molecule and Drug Discovery, Sun Yat-Sen University, Guangzhou510006, China. FAU - Liao, Lihao AU - Liao L AUID- ORCID: 0009-0009-5516-0611 AD - MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, and Guangdong Key Laboratory of Chiral Molecule and Drug Discovery, Sun Yat-Sen University, Guangzhou510006, China. FAU - Peng, Qian AU - Peng Q AUID- ORCID: 0000-0002-1218-5976 AD - State Key Laboratory of Elemento-Organic Chemistry and Tianjin Key Laboratory of Biosensing and Molecular Recognition, College of Chemistry, Frontiers Science Center for New Organic Matter, Nankai University, Tianjin300071, China. AD - Haihe Laboratory of Sustainable Chemical Transformations, Tianjin300192, China. FAU - Zhao, Xiaodan AU - Zhao X AUID- ORCID: 0000-0002-2135-5121 AD - MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, and Guangdong Key Laboratory of Chiral Molecule and Drug Discovery, Sun Yat-Sen University, Guangzhou510006, China. LA - eng GR - 2025A04J4290/Guangzhou Science and Technology Plan, Basic and Applied Basic Research Special Topic, Young Doctoral ?Sailing? Project/ GR - 22371308/National Natural Science Foundation of China (NSFC)/ GR - 22571326/National Natural Science Foundation of China (NSFC)/ GR - 24HHWCSS00019/Haihe Laboratory of Sustainable Chemical Transformation of Tianjin/ GR - 25ZXZSSS00700/Tianjin Major Science and Technology Project for National Key Laboratories/ GR - NA/Frontiers Science Center for New Organic Matter/ GR - NA/Guangdong Basic Research Center of Excellence for Functional Molecular Engineering/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/04/28 00:00 [received] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5450997 [pii] AID - 10.1021/jacs.6c08713 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):45406-45418. doi: 10.1021/jacs.6c08713. PMID- 42954536 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Tailoring Superhydrophobic Ultramicropores in Covalent Organic Frameworks for Humidity-Resistant Methane Purification. PG - 45295-45300 LID - 10.1021/jacs.6c16277 [doi] AB - Efficient purification of methane from raw natural gas under humid conditions remains challenging because water adsorption often degrades the separation performance and structural stability of porous adsorbents. Here we report a topology- and stacking-guided molecular design strategy that integrates ultramicropore engineering with pore-surface fluorination to construct superhydrophobic covalent organic frameworks (COFs) for humidity-resistant methane purification. Two isoreticular fluorinated COFs, TF-COF and NF-COF, were constructed by rationally controlling framework topology and interlayer stacking, generating well-defined ultramicropores for size-selective discrimination of C1-C3 hydrocarbons. Fluorination simultaneously enhances pore-wall hydrophobicity and strengthens host-guest interactions, creating a confined adsorption environment that promotes hydrocarbon recognition while effectively suppressing competitive water adsorption. As a result, NF-COF exhibits a propane uptake of 99.4 cm3 g-1 and an exceptional C3H8/CH4 IAST selectivity of 251.7 at 298 K. Dynamic breakthrough experiments further demonstrate efficient methane purification under both dry and highly humid conditions, with the separation performance fully retained at 97% relative humidity owing to its superhydrophobic surface. Theoretical calculations reveal that densely distributed fluorine atoms generate synergistic pore confinement and multiple C-H...F van der Waals interactions, simultaneously maximizing adsorption capacity and molecular discrimination. These findings establish fluorinated superhydrophobic COFs as a robust platform for energy-efficient natural gas upgrading under industrially relevant humid conditions. CI - (c) 2026 American Chemical Society. FAU - Liu, Jialiang AU - Liu J AD - School of Materials Science and Chemical Engineering, Ningbo University, Ningbo315211, China. FAU - Gong, Chengtao AU - Gong C AUID- ORCID: 0009-0009-6487-8553 AD - College of Materials Science and Engineering, Zhejiang University of Technology, Hangzhou310014, China. FAU - Yuan, Shuxin AU - Yuan S AD - College of Chemistry and Molecular Sciences, Wuhan University, Wuhan430072, China. FAU - Hao, Pengyue AU - Hao P AD - School of Materials Science and Engineering, Wuhan Institute of Technology, Wuhan430205, China. FAU - Li, Xiaohui AU - Li X AD - School of Materials Science and Chemical Engineering, Ningbo Key Laboratory of Low-Carbon Separation and Energy Conversion, Ningbo University, Ningbo315211, China. FAU - Peng, Yongwu AU - Peng Y AUID- ORCID: 0000-0003-4760-5173 LA - eng GR - 432612723/Ningbo University/ GR - 22375179/National Natural Science Foundation of China (NSFC)/ GR - 223B2117/National Natural Science Foundation of China (NSFC)/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/08/04 00:00 [received] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5450603 [pii] AID - 10.1021/jacs.6c16277 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):45295-45300. doi: 10.1021/jacs.6c16277. PMID- 42954534 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Reactivity and Selectivity of Photocatalytic Nitrate Reduction Reaction to Ammonia: Pt, Pd, Ir, and Rh Single Atoms on p-Type TiO2 Nanotubes. PG - 45301-45307 LID - 10.1021/jacs.6c16392 [doi] AB - We present a systematic study of noble metal (Pt, Pd, Ir, and Rh) single-atom cocatalysts decorated on p-type and quantum-confined TiO2 nanotubes for photocatalytic nitrate reduction to ammonia. All single-atom cocatalysts were prepared through a reactive deposition using different metal precursors, resulting in a single-atom oxidation state (delta+) of delta approximately 2. At a similar loading concentration (0.2-0.3 at%) and in the absence of any sacrificial agent, Pd single atoms exhibit the highest element-specific activity for ammonia production, while Pt single atoms display a prominent photocatalytic activity with a strong tendency of nitrite reduction toward ammonia. Rh single atoms achieve the highest ammonia selectivity, whereas Ir single atoms predominantly produce nitrite due to inhibited subsequent reduction. Comparisons with single atoms deposited on conventional TiO2 (P25) and with nanoparticles supported on the nanotubes reveal that both the semiconductor properties (quantum confinement effect and p-type conductivity) and the speciation of the cocatalyst (single atom) can significantly enhance activity and selectivity. These findings aid in establishing design principles for different single-atom catalysts toward efficient and selective photocatalytic nitrogen valorization. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Jung, Hayoon AU - Jung H AD - Department of Materials Science and Engineering, WW4-LKO, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Martensstrasse 7, 91058Erlangen, Germany. AD - Regional Centre of Advanced Technologies and Materials, Czech Advanced Technology and Research Institute (CATRIN), Palacky University Olomouc, Slechtitelu 27, 78371Olomouc, Czech Republic. FAU - Will, Johannes AU - Will J AUID- ORCID: 0000-0003-0326-9913 AD - Institute of Micro- and Nanostructure Research & Center for Nanoanalysis and Electron Microscopy (CENEM), IZNF, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Cauerstrasse 3, 91058Erlangen, Germany. FAU - Langer, Michal AU - Langer M AUID- ORCID: 0000-0003-2574-0980 AD - IT4Innovations, VSB-Technical University of Ostrava, 17. listopadu 2172/15, 70800Ostrava-Poruba, Czech Republic. FAU - Langer, Rostislav AU - Langer R AD - IT4Innovations, VSB-Technical University of Ostrava, 17. listopadu 2172/15, 70800Ostrava-Poruba, Czech Republic. FAU - Kment, Stepan AU - Kment S AUID- ORCID: 0000-0002-6381-5093 AD - Regional Centre of Advanced Technologies and Materials, Czech Advanced Technology and Research Institute (CATRIN), Palacky University Olomouc, Slechtitelu 27, 78371Olomouc, Czech Republic. FAU - Otyepka, Michal AU - Otyepka M AUID- ORCID: 0000-0002-1066-5677 AD - Regional Centre of Advanced Technologies and Materials, Czech Advanced Technology and Research Institute (CATRIN), Palacky University Olomouc, Slechtitelu 27, 78371Olomouc, Czech Republic. AD - IT4Innovations, VSB-Technical University of Ostrava, 17. listopadu 2172/15, 70800Ostrava-Poruba, Czech Republic. FAU - Spiecker, Erdmann AU - Spiecker E AUID- ORCID: 0000-0002-2723-5227 AD - Institute of Micro- and Nanostructure Research & Center for Nanoanalysis and Electron Microscopy (CENEM), IZNF, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Cauerstrasse 3, 91058Erlangen, Germany. FAU - Schmuki, Patrik AU - Schmuki P AUID- ORCID: 0000-0002-9208-5771 AD - Department of Materials Science and Engineering, WW4-LKO, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Martensstrasse 7, 91058Erlangen, Germany. AD - Regional Centre of Advanced Technologies and Materials, Czech Advanced Technology and Research Institute (CATRIN), Palacky University Olomouc, Slechtitelu 27, 78371Olomouc, Czech Republic. LA - eng GR - CZ.10.03.01/00/22_003/0000048/European Commission/ GR - 1452 - 431791331/Deutsche Forschungsgemeinschaft/ GR - 23-08019X/Grantov? Agentura Cesk? Republiky/ GR - 90254/European Regional Development Fund/ GR - CZ.02.01.01/00/22_008/0004587/European Regional Development Fund/ GR - CZ.02.1.01/0.0/0.0/15_003/0000416/European Regional Development Fund/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/08/05 00:00 [received] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/09/17 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5450581 [pii] AID - 10.1021/jacs.6c16392 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):45301-45307. doi: 10.1021/jacs.6c16392. PMID- 42954531 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Giant Baromagnetic Effect Induced by Spin Engineering in Mn3(Zn0.25Sn0.25Ga0.25Ge0.25)C. PG - 45738-45748 LID - 10.1021/jacs.6c13327 [doi] AB - Baromagnetic effect (BME), arising from pressure-induced spin reorientation, holds great potential for next-generation information technologies owing to its strong multifield coupling characteristics. However, the practical implementation of BME is hindered by the small magnetization change and the large driving pressure required in conventional materials. Guided by spin-polarized calculations, this work successfully obtains a giant BME material Mn3(Zn0.25Sn0.25Ga0.25Ge0.25)C, which exhibits a remarkable pressure-induced maximum magnetization change (|DeltaMmax|) of 2.63 muB.f.u.-1 and an exceptionally large baromagnetic coefficient (BMC, d) of 15.0 muB.f.u.-1.GPa-1. Temperature-dependent neutron powder diffraction (NPD) measurements reveal a clear evolution from the nearly collinear antiferromagnetic (cAFM) configuration with k = (1/2, 1/2, 1/2) to the ferromagnetic (FM) configuration, indicating that the spins are highly susceptible to reorientation under external perturbations. First-principles calculations further clarify that pressure modulates the energy difference (DeltaE) between the cAFM and FM configurations via the Ruderman-Kittel-Kasuya-Yosida (RKKY) interaction, elucidating the microscopic origin of the giant BME. This demonstration establishes spin engineering, achieved through precise control of spin configurations via targeted element selection, as an effective strategy for realizing high-performance baromagnetic systems. It further provides clear guidance for designing materials with superior functional properties. CI - (c) 2026 American Chemical Society. FAU - Luo, Jiechen AU - Luo J AD - School of Physics, Beihang University, Beijing100191, China. FAU - Wu, Wentao AU - Wu W AD - Department of Micro-Nano Electronics, College of Electronic Information and Optical Engineering, National Key Laboratory of Semiconductor Laser, Nankai University, Tianjin Key Laboratory of Efficient Utilization of Solar Energy, Engineering Research Center of Thin Film Optoelectronics Technology (Ministry of Education), Tianjin300350, China. FAU - Qiu, Ao AU - Qiu A AUID- ORCID: 0009-0003-2009-5908 AD - School of Physics, Beihang University, Beijing100191, China. AD - Shen Yuan Honors College, Beihang University, Beijing100191, China. FAU - Wang, Bing AU - Wang B AD - School of Physics, Beihang University, Beijing100191, China. FAU - Yuan, Xiuliang AU - Yuan X AD - School of Physics, Beihang University, Beijing100191, China. FAU - An, Shihai AU - An S AUID- ORCID: 0009-0005-2408-0222 AD - School of Physics, Beihang University, Beijing100191, China. FAU - Ma, Zhijie AU - Ma Z AD - School of Physics, Beihang University, Beijing100191, China. FAU - Xu, Zihui AU - Xu Z AD - School of Physics, Beihang University, Beijing100191, China. FAU - Shi, Kewen AU - Shi K AUID- ORCID: 0000-0002-1294-3220 AD - School of Integrated Circuit Science and Engineering, Beihang University, Beijing100191, China. FAU - Deng, Sihao AU - Deng S AD - Spallation Neutron Source Science Center , Dongguan523803, China. AD - Institute of High Energy Physics, Chinese Academy of Sciences, Beijing100049, China. FAU - Xu, Juping AU - Xu J AUID- ORCID: 0000-0002-9576-8391 AD - Spallation Neutron Source Science Center , Dongguan523803, China. AD - Institute of High Energy Physics, Chinese Academy of Sciences, Beijing100049, China. FAU - Yin, Wen AU - Yin W AUID- ORCID: 0000-0002-7110-9471 AD - Spallation Neutron Source Science Center , Dongguan523803, China. AD - Institute of High Energy Physics, Chinese Academy of Sciences, Beijing100049, China. FAU - Wang, Wei-Hua AU - Wang WH AUID- ORCID: 0000-0002-2287-7669 AD - Department of Micro-Nano Electronics, College of Electronic Information and Optical Engineering, National Key Laboratory of Semiconductor Laser, Nankai University, Tianjin Key Laboratory of Efficient Utilization of Solar Energy, Engineering Research Center of Thin Film Optoelectronics Technology (Ministry of Education), Tianjin300350, China. FAU - Wang, Cong AU - Wang C AUID- ORCID: 0000-0002-4100-4222 AD - School of Integrated Circuit Science and Engineering, Beihang University, Beijing100191, China. AD - National Key Lab of Spintronics, Institute of International Innovation, Beihang University, Yuhang, Hangzhou311115, P.R. China. FAU - Sun, Ying AU - Sun Y AUID- ORCID: 0000-0003-2165-1593 AD - School of Physics, Beihang University, Beijing100191, China. LA - eng GR - 52272264/National Natural Science Foundation of China/ GR - 52472273/National Natural Science Foundation of China/ GR - 2022YFA1402600/National Key Research and Development Program of China/ GR - 2025YFF0521102/National Key Research and Development Program of China/ GR - NA/Fundamental Research Funds for the Central Universities/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/06/30 00:00 [received] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/09/23 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5450556 [pii] AID - 10.1021/jacs.6c13327 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):45738-45748. doi: 10.1021/jacs.6c13327. PMID- 42954530 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Divergent Synthesis of Chiral-at-Sulfur Sulfoximines via Photoredox/Rhodium-Catalyzed Enantioselective C-H Activation. PG - 46118-46127 LID - 10.1021/jacs.6c15392 [doi] AB - The merger of photoredox catalysis with transition-metal catalysis has emerged as a powerful platform for sustainable synthesis, yet asymmetric variants of photoinduced transition-metal-catalyzed C-H activation remain largely unexplored. Herein, we report a visible-light-driven Rh(III)/chiral carboxylic acid (CCA) cooperative catalytic system that enables the tunable divergent synthesis of two distinct classes of chiral sulfur-containing scaffolds from common sulfoximine and alkyne substrates. Through precise modulation of the reaction conditions, the reaction can be selectively steered toward either the annulation product or the ring-opened product with high enantioselectivities (up to 97% ee). This work provides the first example of asymmetric photoredox/group 9 CpXM(III)-catalyzed C-H functionalization and highlights reactive oxygen species as programmable selectivity switches in asymmetric catalysis. CI - (c) 2026 American Chemical Society. FAU - Wu, Yan-Xuan AU - Wu YX AD - State Key Laboratory of Soil Pollution Control and Safety, Department of Chemistry, Zhejiang University, Hangzhou310058, China. FAU - Hu, Jia-Heng AU - Hu JH AD - State Key Laboratory of Soil Pollution Control and Safety, Department of Chemistry, Zhejiang University, Hangzhou310058, China. FAU - Xu, Yuan AU - Xu Y AD - State Key Laboratory of Soil Pollution Control and Safety, Department of Chemistry, Zhejiang University, Hangzhou310058, China. FAU - Qian, Pu-Fan AU - Qian PF AUID- ORCID: 0000-0003-2065-3669 AD - State Key Laboratory of Soil Pollution Control and Safety, Department of Chemistry, Zhejiang University, Hangzhou310058, China. FAU - Kong, Ke-Xin AU - Kong KX AD - State Key Laboratory of Soil Pollution Control and Safety, Department of Chemistry, Zhejiang University, Hangzhou310058, China. FAU - Yao, Qi-Jun AU - Yao QJ AUID- ORCID: 0000-0002-2872-7688 AD - State Key Laboratory of Soil Pollution Control and Safety, Department of Chemistry, Zhejiang University, Hangzhou310058, China. FAU - Zhou, Tao AU - Zhou T AUID- ORCID: 0000-0001-7258-2845 AD - State Key Laboratory of Soil Pollution Control and Safety, Department of Chemistry, Zhejiang University, Hangzhou310058, China. FAU - Shi, Bing-Feng AU - Shi BF AUID- ORCID: 0000-0003-0375-955X AD - State Key Laboratory of Soil Pollution Control and Safety, Department of Chemistry, Zhejiang University, Hangzhou310058, China. LA - eng GR - 22271250/National Natural Science Foundation of China/ GR - 22525011/National Natural Science Foundation of China/ GR - U22A20388/National Natural Science Foundation of China/ GR - U25A20547/National Natural Science Foundation of China/ GR - JYB2025XDXM407/Ministry of Education of the People's Republic of China/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/07/25 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/09/09 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5450235 [pii] AID - 10.1021/jacs.6c15392 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):46118-46127. doi: 10.1021/jacs.6c15392. PMID- 42954528 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Macrocycle Screening against the C-Terminal Region of CHD4 Uncovers Its Role as an Interaction Hub in the Formation of the Nucleosome Remodeling and Deacetylase Complex. PG - 45592-45599 LID - 10.1021/jacs.6c11754 [doi] AB - The Nucleosome Remodeling and Deacetylase complex (NuRD) plays a key role in regulating hemoglobin expression in adult erythroid cells. Selectively disrupting this complex potently induces the expression of fetal hemoglobin, a proven therapeutic strategy for treating beta-hemoglobinopathies such as sickle cell anemia. In these studies, we have used mRNA display to identify small macrocyclic peptides that inhibit the interaction between two core components of NuRD, the SANT-SLIDE domain of CHD4 and the CR2 domain of GATAD2A. In addition, the screen suggested a second binding site on the CHD4 domain. Based on this observation, we hypothesized and confirmed that CDK2AP1 bound to this region of CHD4, leading us to purify and determine the structure of the ternary complex between CHD4, GATAD2A, and CDK2AP1. The results of our studies show that the SANT-SLIDE domain of CHD4 functions as a critical interaction hub in the formation of NuRD and suggest a strategy to block NuRD function for therapy. CI - (c) 2026 American Chemical Society. FAU - Williams, David C Jr AU - Williams DC Jr AUID- ORCID: 0000-0002-6536-4038 AD - Department of Pathology and Laboratory Medicine and Lineberger Comprehensive Cancer Center, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina27599, United States. FAU - Ren, Jie AU - Ren J AD - Center for Integrative Chemical Biology and Drug Discovery, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina27599, United States. AD - Division of Chemical Biology, and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina27599, United States. FAU - Li, Torry AU - Li T AUID- ORCID: 0009-0005-5708-502X AD - Department of Pathology and Laboratory Medicine and Lineberger Comprehensive Cancer Center, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina27599, United States. FAU - Pelton, Jarrett M AU - Pelton JM AUID- ORCID: 0000-0002-5483-9915 AD - Center for Integrative Chemical Biology and Drug Discovery, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina27599, United States. AD - Division of Chemical Biology, and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina27599, United States. FAU - Dedakia, Dhanushi AU - Dedakia D AD - Department of Pathology and Laboratory Medicine and Lineberger Comprehensive Cancer Center, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina27599, United States. FAU - Novy, Brandon AU - Novy B AD - Center for Integrative Chemical Biology and Drug Discovery, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina27599, United States. AD - Curriculum in Bioinformatics and Computational Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina27599, United States. FAU - Popov, Konstantin I AU - Popov KI AD - Center for Integrative Chemical Biology and Drug Discovery, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina27599, United States. AD - Division of Chemical Biology, and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina27599, United States. FAU - McGinty, Robert K AU - McGinty RK AUID- ORCID: 0000-0001-9641-7350 AD - Center for Integrative Chemical Biology and Drug Discovery, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina27599, United States. AD - Division of Chemical Biology, and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina27599, United States. AD - Department of Biochemistry and Biophysics and Lineberger Comprehensive Cancer Center, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina27599, United States. FAU - Ginder, Gordon D AU - Ginder GD AD - Departments of Internal Medicine, Cellular, Molecular, and Genetic Medicine, and Microbiology and Immunology, and the Massey Cancer Center, Virginia Commonwealth University, Richmond, Virginia23298, United States. FAU - Bowers, Albert A AU - Bowers AA AUID- ORCID: 0000-0001-8214-7484 AD - Center for Integrative Chemical Biology and Drug Discovery, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina27599, United States. AD - Division of Chemical Biology, and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina27599, United States. LA - eng GR - P30CA016086/National Institutes of Health (NIH)/ GR - R01DK115563/National Institutes of Health (NIH)/ GR - R35GM125005/National Institutes of Health (NIH)/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 RN - EC 3.5.1.98 (Mi-2 Nucleosome Remodeling and Deacetylase Complex) RN - 0 (CHD4 protein, human) RN - 0 (Nucleosomes) SB - IM MH - *Mi-2 Nucleosome Remodeling and Deacetylase Complex/chemistry/metabolism/antagonists & inhibitors MH - Humans MH - *Nucleosomes/metabolism MH - Models, Molecular EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/06/08 00:00 [received] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5450204 [pii] AID - 10.1021/jacs.6c11754 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):45592-45599. doi: 10.1021/jacs.6c11754. PMID- 42954525 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Probing the Conformational Behavior of a Seven Hetero-Spin Supramolecule. PG - 45399-45405 LID - 10.1021/jacs.6c08331 [doi] AB - The synthesis and crystal structure of a 7-spin supramolecular poly-rotaxane is reported. The [4]rotaxane structure involves a trigonal thread centred on a CrNi2 core, with each of the three arms terminated by Cu(II) centres and bearing a Cr7Ni metallocycle. The macromolecule brings together three distinct electron spin centers. Continuous-wave electron paramagnetic resonance spectroscopy confirms the molecule remains intact in solution, with the three types of spin center each uniquely addressable. Orientation-selective Double Electron Electron Resonance (DEER) and Relaxation Induced Dipolar Modulation Enhancement (RIDME) spectroscopies are used to selectively probe the different spin-spin interactions in the frozen solution phase and to define differences from the solid-state crystal structure. CI - (c) 2026 American Chemical Society. FAU - Loci, Lubomir AU - Loci L AD - Department of Chemistry, The University of Manchester, Oxford Road, ManchesterM13 9PL, United Kingdom. AD - The National Research Facility for Electron Paramagnetic Resonance, The Photon Science Institute, The University of Manchester, Oxford Road, ManchesterM13 9PL, United Kingdom. FAU - Bennett, Tom S AU - Bennett TS AD - Department of Chemistry, The University of Manchester, Oxford Road, ManchesterM13 9PL, United Kingdom. FAU - Lockyer, Selena J AU - Lockyer SJ AD - Department of Chemistry, The University of Manchester, Oxford Road, ManchesterM13 9PL, United Kingdom. AD - The National Research Facility for Electron Paramagnetic Resonance, The Photon Science Institute, The University of Manchester, Oxford Road, ManchesterM13 9PL, United Kingdom. FAU - Whitehead, George F S AU - Whitehead GFS AUID- ORCID: 0000-0003-1949-4250 AD - Department of Chemistry, The University of Manchester, Oxford Road, ManchesterM13 9PL, United Kingdom. FAU - Timco, Grigore A AU - Timco GA AUID- ORCID: 0000-0003-0966-0315 AD - Department of Chemistry, The University of Manchester, Oxford Road, ManchesterM13 9PL, United Kingdom. FAU - Da Ros, Giulia AU - Da Ros G AD - Department of Chemistry, The University of Manchester, Oxford Road, ManchesterM13 9PL, United Kingdom. AD - The National Research Facility for Electron Paramagnetic Resonance, The Photon Science Institute, The University of Manchester, Oxford Road, ManchesterM13 9PL, United Kingdom. FAU - Rogers, Ciaran J AU - Rogers CJ AD - Department of Chemistry, The University of Manchester, Oxford Road, ManchesterM13 9PL, United Kingdom. FAU - Brookfield, Adam AU - Brookfield A AD - The National Research Facility for Electron Paramagnetic Resonance, The Photon Science Institute, The University of Manchester, Oxford Road, ManchesterM13 9PL, United Kingdom. FAU - McInnes, Eric J L AU - McInnes EJL AUID- ORCID: 0000-0002-4090-7040 AD - Department of Chemistry, The University of Manchester, Oxford Road, ManchesterM13 9PL, United Kingdom. AD - The National Research Facility for Electron Paramagnetic Resonance, The Photon Science Institute, The University of Manchester, Oxford Road, ManchesterM13 9PL, United Kingdom. FAU - Winpenny, Richard E P AU - Winpenny REP AUID- ORCID: 0000-0002-7101-3963 AD - Department of Chemistry, The University of Manchester, Oxford Road, ManchesterM13 9PL, United Kingdom. AD - The National Research Facility for Electron Paramagnetic Resonance, The Photon Science Institute, The University of Manchester, Oxford Road, ManchesterM13 9PL, United Kingdom. FAU - Bowen, Alice M AU - Bowen AM AUID- ORCID: 0000-0002-6413-2841 AD - Department of Chemistry, The University of Manchester, Oxford Road, ManchesterM13 9PL, United Kingdom. AD - The National Research Facility for Electron Paramagnetic Resonance, The Photon Science Institute, The University of Manchester, Oxford Road, ManchesterM13 9PL, United Kingdom. LA - eng GR - DH160004/Royal Society/ GR - DHF\R\221018/Royal Society/ GR - RGF\EA\180287/Royal Society/ GR - CAMS ACTF ref. 600310/09/Royal Society of Chemistry/ GR - Dame Kathleen Ollerenshaw Fellowship/University of Manchester/ GR - Dean's Doctoral Award for Lubomir Loci/University of Manchester/ GR - PDRA support for Lubomir Loci/University of Manchester/ GR - PhD stipend for Giulia Da Ros/University of Manchester/ GR - ERC-2017-ADG-786734/ERC_/European Research Council/International GR - DH160004/Engineering and Physical Sciences Research Council (EPSRC)/ GR - DHF\R\221018/Engineering and Physical Sciences Research Council (EPSRC)/ GR - EP/P001386/1/Engineering and Physical Sciences Research Council (EPSRC)/ GR - EP/S033181/1/Engineering and Physical Sciences Research Council (EPSRC)/ GR - EP/T019328/1/Engineering and Physical Sciences Research Council (EPSRC)/ GR - EP/V035231/1/Engineering and Physical Sciences Research Council (EPSRC)/ GR - EP/W014521/1/Engineering and Physical Sciences Research Council (EPSRC)/ GR - EP/X034623/1/Engineering and Physical Sciences Research Council (EPSRC)/ GR - EP/Y01748X/1/Engineering and Physical Sciences Research Council (EPSRC)/ GR - EP/Z535801/1/Engineering and Physical Sciences Research Council (EPSRC)/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/04/22 00:00 [received] PHST- 2026/09/10 00:00 [accepted] PHST- 2026/09/09 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5449648 [pii] AID - 10.1021/jacs.6c08331 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):45399-45405. doi: 10.1021/jacs.6c08331. PMID- 42954519 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Entropy-Driven Dynamic Oxygen Cycling on High-Entropy Oxides Enables Coke-Resistant Dry Reforming of Methane. PG - 45880-45891 LID - 10.1021/jacs.6c14327 [doi] AB - Catalyst deactivation caused by carbon deposition and metal sintering remains a major challenge for dry reforming of methane (DRM). Herein, we designed and synthesized a Ce-based high-entropy oxide catalyst (NiCoMgAlZrCe-HEO). Configurational entropy stabilizes a multication-disordered lattice with pronounced local distortion, which modulates the oxygen coordination environment and enhances the lattice-oxygen reactivity. The NiCo alloy nanoparticles formed through in situ reductive exsolution are anchored to the HEO matrix, thereby enhancing the resistance to sintering. The reduced NiCoMgAlZrCe-HEO exhibits CH4 and CO2 conversions of 80.2% and 87.3%, respectively, at 700 degrees C and maintains stable catalytic performance over 500 h. Combined experimental characterization and density functional theory calculations reveal a dynamic oxygen-cycling mechanism enabled by the high-entropy structure. Mobile lattice oxygen favors the selective activation of CH4 by promoting the formation of *CH3O intermediates, thereby suppressing the carbon-forming pathways. Meanwhile, abundant oxygen vacancies facilitate CO2 activation, accelerating the replenishment of the reactive lattice oxygen. This work offers a feasible strategy for designing DRM catalysts that are resistant to coking and sintering based on configurational entropy regulation. CI - (c) 2026 American Chemical Society. FAU - Wang, Zhenpeng AU - Wang Z AD - State Key Laboratory of Heavy Oil Processing, College of Science, China University of Petroleum, Beijing102249, P. R. China. FAU - Qin, Tian AU - Qin T AUID- ORCID: 0000-0002-2856-4743 AD - School of Chemistry and Chemical Engineering, In-situ Center for Physical Sciences, Frontiers Science Center for Transformative Molecules, Shanghai Jiao Tong University, Shanghai200240, P. R. China. FAU - Xiong, Jing AU - Xiong J AD - State Key Laboratory of Heavy Oil Processing, College of Science, China University of Petroleum, Beijing102249, P. R. China. FAU - Yang, Yitao AU - Yang Y AD - State Key Laboratory of Heavy Oil Processing, College of Science, China University of Petroleum, Beijing102249, P. R. China. FAU - Han, Dawei AU - Han D AD - State Key Laboratory of Heavy Oil Processing, College of Science, China University of Petroleum, Beijing102249, P. R. China. FAU - Li, Xuanzhen AU - Li X AD - State Key Laboratory of Heavy Oil Processing, College of Science, China University of Petroleum, Beijing102249, P. R. China. FAU - Guo, Hao AU - Guo H AD - State Key Laboratory of Heavy Oil Processing, College of Science, China University of Petroleum, Beijing102249, P. R. China. FAU - Yang, Chenxi AU - Yang C AD - Multi-disciplinary Research Division, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing100049, P. R. China. AD - University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing100049, P. R. China. FAU - Liu, Yunpeng AU - Liu Y AUID- ORCID: 0000-0002-3783-0054 AD - Multi-disciplinary Research Division, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing100049, P. R. China. AD - University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing100049, P. R. China. FAU - Xie, Pengfei AU - Xie P AUID- ORCID: 0000-0002-7644-9934 AD - Zhejiang Province Key Laboratory of Advanced Information Materials, Zhejiang University, Hangzhou310058, P. R. China. FAU - Liu, Xi AU - Liu X AUID- ORCID: 0000-0002-8654-0774 AD - School of Chemistry and Chemical Engineering, In-situ Center for Physical Sciences, Frontiers Science Center for Transformative Molecules, Shanghai Jiao Tong University, Shanghai200240, P. R. China. AD - School of Chemistry and Chemical Engineering, Ningxia University, Yinchuan750021, P. R. China. FAU - Zhao, Zhen AU - Zhao Z AUID- ORCID: 0000-0003-0044-5512 AD - State Key Laboratory of Heavy Oil Processing, College of Science, China University of Petroleum, Beijing102249, P. R. China. FAU - Wei, Yuechang AU - Wei Y AUID- ORCID: 0000-0002-1516-5778 AD - State Key Laboratory of Heavy Oil Processing, College of Science, China University of Petroleum, Beijing102249, P. R. China. LA - eng GR - 2025B01010/Government of Xinjiang Uygur Autonomous Region of China/ GR - 12305372/National Natural Science Foundation of China (NSFC)/ GR - 22278365/National Natural Science Foundation of China (NSFC)/ GR - 22376217/National Natural Science Foundation of China (NSFC)/ GR - CNIF20240106/Shandong Institute of Petroleum and Chemical Technology/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/07/12 00:00 [received] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5449460 [pii] AID - 10.1021/jacs.6c14327 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):45880-45891. doi: 10.1021/jacs.6c14327. PMID- 42954514 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - High-Entropy Lanthanoid Oxide Catalysts for Oxidative Coupling of Methane. PG - 46075-46088 LID - 10.1021/jacs.6c15276 [doi] AB - High-entropy engineering of metal oxides provides a powerful platform for developing highly active and structurally robust catalysts; however, its application to heterogenous catalysis that requires stringent selectivity control remains largely unexplored. The low-temperature oxidative coupling of methane (OCM) demands catalysts that combine structural stability with precise tuning of surface basic strength. Here, we report a high-entropy lanthanoid oxide catalyst, (LaSmEuGdDy)0.4O3, synthesized via a sol-gel method using lanthanoid acetates and aspartic acid. The catalyst afforded ethylene and ethane in approximately 12.3% total yield at temperatures less than 600 degrees C and maintained its catalytic activity for 240 h at 600 degrees C. Comparative studies with single-component lanthanoid oxides revealed that the high-entropy catalyst lowers the OCM onset temperature and markedly enhances catalyst durability. CO2 temperature-programmed desorption demonstrated that the surface basic strength of the high-entropy oxide can be systematically controlled through the average ionic radius, promoting the formation of moderately basic sites essential for low-temperature C-H activation. X-ray diffraction and photoelectron spectroscopy further showed that C-type rare-earth oxides exhibit superior resistance to transformation into less-active Ln2O2CO3 compared with A-type oxides and that the high-entropy configuration mitigates the progressive depletion of surface basic strength during prolonged OCM operation. These findings establish high-entropy design as an effective strategy for simultaneously controlling structural stability and surface functionality, opening new opportunities for selective heterogeneous catalysis. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Takahashi, Rena AU - Takahashi R AD - Materials and Structures Laboratory, Institute of Integrated Research, Institute of Science Tokyo, Nagatsuta-cho 4259-R3-6, Midori-ku, Yokohama-city, Kanagawa226-8501, Japan. FAU - Wachi, Keiju AU - Wachi K AD - Materials and Structures Laboratory, Institute of Integrated Research, Institute of Science Tokyo, Nagatsuta-cho 4259-R3-6, Midori-ku, Yokohama-city, Kanagawa226-8501, Japan. FAU - Kamata, Keigo AU - Kamata K AUID- ORCID: 0000-0002-0624-8483 AD - Materials and Structures Laboratory, Institute of Integrated Research, Institute of Science Tokyo, Nagatsuta-cho 4259-R3-6, Midori-ku, Yokohama-city, Kanagawa226-8501, Japan. LA - eng GR - 24H00393/Japan Society for the Promotion of Science/ GR - 24K23098/Japan Society for the Promotion of Science/ GR - NA/Ministry of Education, Culture, Sports, Science and Technology/ GR - JPMJCR22O1/Japan Science and Technology Agency/ GR - NA/Collaborative Research Projects of Materials and Structures Laboratory, Institute of Integrated Research, Institute of Science Tokyo/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/07/23 00:00 [received] PHST- 2026/08/28 00:00 [accepted] PHST- 2026/08/26 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5446663 [pii] AID - 10.1021/jacs.6c15276 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):46075-46088. doi: 10.1021/jacs.6c15276. PMID- 42954511 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Nonequilibrium Navigation of a Rugged Energy Landscape Enables the Formation of Face-On Single-Domain Dodecagonal Quasicrystals. PG - 45457-45467 LID - 10.1021/jacs.6c09814 [doi] AB - Metastable intermediates play an important role in directing structural evolution across complex energy landscapes. Here, we establish the dodecagonal quasicrystal (DDQC) as a tunable metastable kinetic junction in a single-component carboxyl-functionalized dendritic assembly. Time-resolved small-angle X-ray scattering (SAXS) reveals that this DDQC junction bifurcates into distinct Frank-Kasper (FK) phases through competing kinetic pathways: a deformation-dominated route to the A15 phase at lower temperatures or a mass-exchange-mediated transformation to the thermodynamically favored sigma phase at higher temperatures. By introducing amine-mediated salt bridges to rigidify the micellar cores, we selectively suppress the deformation-driven A15 pathway while preserving access to the sigma phase, thereby programming the bifurcation behavior of the DDQC junction. Leveraging this core engineering, a vapor-induced self-assembly (VISA) strategy was further developed to enable a nonequilibrium sigma-to-DDQC transition. The high nucleation barrier inherent to this nonequilibrium pathway creates a nucleation-controlled regime that suppresses spontaneous polydomain formation, enabling the growth of macroscopically aligned, face-on single-domain DDQC with persistent 12-fold symmetry over millimeter length scales. These findings demonstrate that micellar core engineering provides a powerful platform for navigating rugged energy landscapes, transforming metastability from an uncontrolled kinetic trap into a programmable element for deterministic pathway selection and long-range quasiperiodic order in soft matter. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Egashira, Mayumi AU - Egashira M AUID- ORCID: 0000-0002-2382-3128 AD - Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd, Taipei10617, Taiwan. FAU - Chen, Jing-Wei AU - Chen JW AD - Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd, Taipei10617, Taiwan. FAU - Wang, Mao-Yan AU - Wang MY AD - Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd, Taipei10617, Taiwan. FAU - Su, Chun-Jen AU - Su CJ AD - National Synchrotron Radiation Research Center , 101 Hsin-Ann Road, Hsinchu30076, Taiwan. FAU - Yeh, Yi-Qi AU - Yeh YQ AUID- ORCID: 0000-0002-2387-3600 AD - National Synchrotron Radiation Research Center , 101 Hsin-Ann Road, Hsinchu30076, Taiwan. FAU - Chuang, Wei-Tsung AU - Chuang WT AUID- ORCID: 0000-0002-9000-2194 AD - National Synchrotron Radiation Research Center , 101 Hsin-Ann Road, Hsinchu30076, Taiwan. FAU - Wang, Chien-Lung AU - Wang CL AUID- ORCID: 0000-0002-4799-4730 AD - Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd, Taipei10617, Taiwan. LA - eng GR - NSTC 113-2123-M-002-008/National Science and Technology Council/ GR - NSTC 114-2123-M-002-005/National Science and Technology Council/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/05/13 00:00 [received] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5442171 [pii] AID - 10.1021/jacs.6c09814 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):45457-45467. doi: 10.1021/jacs.6c09814. PMID- 42954497 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) VI - 148 IP - 42 DP - 2026 Oct 28 TI - Expanding the Hydrocarbon Landscape: Stereoselective Synthesis of Functionalized ttt-[13]Graphanes. PG - 45610-45623 LID - 10.1021/jacs.6c12162 [doi] AB - Despite the significant interest in rigid, sp3-rich 3D hydrocarbon frameworks in medicinal and materials chemistry, the [13]graphanyl scaffold has remained synthetically prohibitive and, consequently, underexplored. Herein, we report a scalable, de novo stereoselective synthesis of the ttt-[13]graphanyl architecture utilizing a key dienyne metathesis cascade. To overcome the profound steric shielding at the central carbon vertex, we harnessed radical-mediated photocatalytic decarboxylation to install diverse functional groups, generating a library of centrally derivatized ttt-[13]G analogues. Complementary peripheral functionalization was also achieved via site-selective MHAT chemistry. Density functional theory (DFT) calculations rationalized the empirical reactivity trends, mapping the competitive landscape between Giese additions and hydrogen-atom transfer quenching. Finally, X-ray crystallographic analyses confirmed a substituent-dependent "doming" distortion of the rigid tricyclic framework. Together, these results provide a synthetic foundation to incorporate the [13]graphane architecture into advanced functional molecules, offering a new 3D node for rational molecular design. CI - (c) 2026 American Chemical Society. FAU - Chu, Alex K H AU - Chu AKH AD - Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong999077, China. FAU - Ng, Elvis Wang Hei AU - Ng EWH AD - Department of Pharmacology and Pharmacy, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam Road, Hong Kong999077, China. FAU - Rizzo, Antonio AU - Rizzo A AD - Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong999077, China. AD - State Key Laboratory of Synthetic Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong999077, China. FAU - Chiu, Pauline AU - Chiu P AD - Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong999077, China. AD - State Key Laboratory of Synthetic Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong999077, China. LA - eng GR - PF21-64496/Research Grants Council, University Grants Committee/ GR - 5633576/University of Hong Kong/ GR - NA/State Key Laboratory for Synthetic Chemistry/ PT - Journal Article PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 09:14 PHST- 2026/06/13 00:00 [received] PHST- 2026/08/07 00:00 [accepted] PHST- 2026/07/28 00:00 [revised] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:14 [entrez] AID - 5268839 [pii] AID - 10.1021/jacs.6c12162 [doi] PST - ppublish SO - J Am Chem Soc. 2026 Oct 28;148(42):45610-45623. doi: 10.1021/jacs.6c12162. PMID- 42954484 OWN - NLM STAT- Publisher LR - 20261029 IS - 1098-5514 (Electronic) IS - 0022-538X (Linking) DP - 2026 Oct 29 TI - A seven-valent norovirus mRNA vaccine elicits robust humoral responses in preclinical models. PG - e0112826 LID - 10.1128/jvi.01128-26 [doi] AB - Noroviruses are a leading cause of acute gastroenteritis and impose a substantial global disease burden, particularly in children younger than 5 years and in older adults. Although multiple vaccine candidates are in preclinical and clinical development, no targeted intervention has been approved. We previously reported that a pentavalent mRNA vaccine candidate encoding VP1 proteins from genotypes GI.1, GII.2, GII.3, GII.4, and GII.6 elicited robust humoral and cellular immune responses in mice and nonhuman primates. Because recent surveillance data indicate increasing prevalence of GI.3 and GII.17, we designed mRNAs targeting these genotypes and evaluated an expanded seven-valent formulation in preclinical studies. We first compared candidate GI.3 and GII.17 strains in mice and selected vaccine components based on immunogenicity and cross-reactivity. The resulting seven-valent mRNA vaccine elicited strong humoral responses in mice and African green monkeys, including serum IgG binding to norovirus virus-like particles (VLPs) and functional antibodies that blocked VLP binding to histo-blood group antigens (HBGAs). Importantly, incorporation of GI.3 and GII.17 broadened functional coverage, whereas multivalent formulation introduced only limited evidence of immune interference. These findings support the feasibility of surveillance-guided updating of multivalent norovirus mRNA vaccines and highlight the adaptability of the mRNA platform for addressing emerging variants.IMPORTANCENorovirus is a major cause of vomiting and diarrhea worldwide, but developing a broadly protective vaccine is difficult because many genetically distinct strains circulate, and their prevalence changes over time. This study demonstrates a flexible vaccine strategy in which mRNA components can be selected and added based on disease surveillance and experimental evidence. We expanded a five-component norovirus vaccine to include GI.3 and GII.17, two increasingly important genotypes. The resulting seven-component vaccine generated strong antibody responses in mice and African green monkeys and broadened the range of virus types targeted by functional antibodies. Although additional studies are needed to determine whether these responses protect humans, the findings show how the adaptability of mRNA technology could support periodic updates of multivalent norovirus vaccines as circulating strains change. FAU - Bystol, Karin AU - Bystol K AD - Department of Infectious Diseases and Vaccines, Merck & Co., Inc., West Point, Pennsylvania, USA. FAU - O'Donnell, Gregory AU - O'Donnell G AD - Department of Quantitative Biosciences, Merck & Co., Inc., West Point, Pennsylvania, USA. ROR: https://ror.org/010brsj79 FAU - Hartwig, Susanne AU - Hartwig S AD - Department of Biostatistics and Research Decision Sciences, Epidemiology, MSD France, Lyon, France. FAU - David, Courtney AU - David C AD - Department of Discovery Pharmaceutical Sciences, Merck & Co., Inc., West Point, Pennsylvania, USA. FAU - Galli, Jennifer AU - Galli J AUID- ORCID: 0000-0001-8577-4790 AD - Department of Infectious Diseases and Vaccines, Merck & Co., Inc., West Point, Pennsylvania, USA. FAU - Warren, Christopher AU - Warren C AD - Department of Infectious Diseases and Vaccines, Merck & Co., Inc., West Point, Pennsylvania, USA. FAU - Go, Gwenny AU - Go G AD - Department of Infectious Diseases and Vaccines, Merck & Co., Inc., West Point, Pennsylvania, USA. FAU - Obed, Bennett AU - Obed B AD - Department of Infectious Diseases and Vaccines, Merck & Co., Inc., West Point, Pennsylvania, USA. FAU - Lago, Athena AU - Lago A AD - Department of Infectious Diseases and Vaccines, Merck & Co., Inc., West Point, Pennsylvania, USA. FAU - Citron, Michael AU - Citron M AD - Department of Infectious Diseases and Vaccines, Merck & Co., Inc., West Point, Pennsylvania, USA. FAU - Fulton, Corey May AU - Fulton CM AD - Department of Quantitative Biosciences, Merck & Co., Inc., West Point, Pennsylvania, USA. ROR: https://ror.org/010brsj79 FAU - Phan, Thienlong AU - Phan T AD - Department of Infectious Diseases and Vaccines, Merck & Co., Inc., West Point, Pennsylvania, USA. FAU - Gaspar, John M AU - Gaspar JM AD - Department of Data Science and Scientific Informatics, Merck & Co., Inc., West Point, Pennsylvania, USA. FAU - Singh, Pratap AU - Singh P AD - Department of Research and Development Sciences (RaDS) IT, Merck & Co., Inc., Rahway, New Jersey, USA. FAU - Fridman, Arthur AU - Fridman A AD - Department of Data Science and Scientific Informatics, Merck & Co., Inc., West Point, Pennsylvania, USA. FAU - Shu, Yi AU - Shu Y AD - Department of Infectious Diseases and Vaccines, Merck & Co., Inc., West Point, Pennsylvania, USA. FAU - Wei, Jiajie AU - Wei J AUID- ORCID: 0000-0001-5415-0395 AD - Department of Infectious Diseases and Vaccines, Merck & Co., Inc., West Point, Pennsylvania, USA. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 SB - IM OTO - NOTNLM OT - humoral response OT - mRNA vaccine OT - norovirus OT - preclinical models EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:05 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:05 [entrez] AID - 10.1128/jvi.01128-26 [doi] PST - aheadofprint SO - J Virol. 2026 Oct 29:e0112826. doi: 10.1128/jvi.01128-26. PMID- 42954483 OWN - NLM STAT- Publisher LR - 20261029 IS - 2150-7511 (Electronic) DP - 2026 Oct 29 TI - Ablation of a maternal Cryptosporidium mRNA-binding protein results in sterile sporozoites. PG - e0199126 LID - 10.1128/mbio.01991-26 [doi] AB - Infection with Cryptosporidium is a leading cause of diarrheal disease and early childhood mortality. This apicomplexan parasite undergoes asexual and sexual replication within the same host, and recent studies have shown an intrinsic developmental program of obligate transition to male and female gametes and sex. While factors were identified that control male fate and development, how female gene expression is orchestrated remains largely unknown. Here, we use the Cryptosporidium Single Cell Atlas to discover an RNA-binding protein (F-RBP) as one of the earliest markers of female identity in C. parvum. Reporter parasites engineered based on this gene allowed us to calibrate transcriptional pseudotime against the real time of female development, revealing a significant window of transcriptional fate ambiguity. While F-RBP is an early transcript, the protein persists throughout female development and into the zygote. Conditional ablation of the F-RBP gene showed it to be dispensable for sex determination and early female development in vitro. However, the gene is essential in vivo, and its loss results in rapid cure. Cell biological experiments link this loss to the production of oocysts that release sporozoites incapable of host-cell invasion. F-RBP binds transcripts highly expressed in the female gamete, enriched for a sequence motif recognized by a Y-box protein family member, and forms mRNA-protein complexes in late females akin to processing or P bodies. We propose F-RBP's essential role to be in the regulation of long-term homeostasis of maternally inherited RNA required for sporozoite infectivity.IMPORTANCEThe parasite Cryptosporidium is responsible for millions of cases of severe diarrheal disease in young children, and the infection is both a consequence of and a risk factor for chronic malnutrition. There are no vaccines available, and a single approved treatment is largely ineffective. Infection relies on ingestion of the oocyst, an environmentally stable spore, which contains an invasive form of the parasite, the sporozoite. Oocysts and sporozoites are the products of parasite sex and the fusion of female and male Cryptosporidium parasites. Here, we report the critical role of an RNA-binding protein in female mRNA homeostasis. Maternal transcripts orchestrate the complex process of sexual replication and oocyst formation. Loss of the protein results in spores that fail to initiate the next round of the life cycle, and conditional ablation using a small-molecule trigger quickly cures infected animals, demonstrating the essential role of parasite sex in continued infection. FAU - Daniels, Abigail M AU - Daniels AM AUID- ORCID: 0000-0002-6542-1960 AD - Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA. RINGGOLD: 6572. ROR: https://ror.org/00b30xv10 FAU - Shaw, Sebastian AU - Shaw S AUID- ORCID: 0000-0001-7163-5650 AD - Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA. RINGGOLD: 6572. ROR: https://ror.org/00b30xv10 FAU - Notzel, Christopher AU - Notzel C AD - Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA. RINGGOLD: 6572. ROR: https://ror.org/00b30xv10 FAU - Xiao, Rui AU - Xiao R AD - Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA. RINGGOLD: 6572. ROR: https://ror.org/00b30xv10 FAU - O'Dea, Keenan AU - O'Dea K AD - Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA. RINGGOLD: 6572. ROR: https://ror.org/00b30xv10 FAU - Beiting, Daniel P AU - Beiting DP AUID- ORCID: 0000-0002-2865-4589 AD - Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA. RINGGOLD: 6572. ROR: https://ror.org/00b30xv10 FAU - Striepen, Boris AU - Striepen B AUID- ORCID: 0000-0002-7426-432X AD - Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA. RINGGOLD: 6572. ROR: https://ror.org/00b30xv10 LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - mBio JT - mBio JID - 101519231 SB - IM OTO - NOTNLM OT - Cryptosporidium OT - RNA-binding proteins OT - apicomplexan parasites OT - diarrhea OT - female gamete OT - intracellular parasites OT - parasite sex EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:05 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:05 [entrez] AID - 10.1128/mbio.01991-26 [doi] PST - aheadofprint SO - mBio. 2026 Oct 29:e0199126. doi: 10.1128/mbio.01991-26. PMID- 42954482 OWN - NLM STAT- Publisher LR - 20261029 IS - 2150-7511 (Electronic) DP - 2026 Oct 29 TI - Targeted CPSF6 recoding reveals global fitness advantages of SPAD integration targeting and cell type-specific roles for capsid binding in HIV-1 DNA synthesis and nuclear import. PG - e0227626 LID - 10.1128/mbio.02276-26 [doi] AB - As part of the CFIm complex, CPSF6 regulates cellular gene expression via alternative polyadenylation. CPSF6 binds primate lentiviral capsids and has been implicated in HIV-1 reverse transcription, nuclear import, and integration targeting. However, interpretations of the role(s) of CPSF6 during HIV-1 replication are complicated by the transcriptional changes that occur when the protein is depleted from cells. To disentangle the role of CPSF6 in HIV-1 replication from alternative polyadenylation, we selectively recoded its capsid-binding phenylalanine-glycine motif to alanine-alanine in CD4-positive Jurkat T cells, CEM12D7 T cells, and THP-1 myeloid/macrophage cells using CRISPR/Cas9-based homology-directed repair. While large variations in single-round HIV-1 infection were observed among isolated T cell clones, en masse comparisons of recoded T cells to cloned control T cells failed to reveal any significant differences in infection levels. Consistent with an overall fitness advantage provided by DNA integration in speckle-associated domains, which was ablated by recoding across cell types, HIV-1 replicated more robustly in control T cell populations. Single-round infection, in addition to spreading replication, was reduced in recoded myeloid/macrophage cells, which correlated with reductions in reverse transcription, and more robust blocks to HIV-1 nuclear import. Recoded monocytes/macrophages, however, did not reveal any evidence for enhanced type I interferon production or sensing of HIV-1 nucleic acids. Altogether, we define endogenously recoded cell lines to uncouple capsid binding from CPSF6's role in RNA metabolism, which highlights a global fitness advantage of targeted DNA integration and cell type-specific roles for CPSF6 in HIV-1 reverse transcription, nuclear import, and virus replication proficiency.IMPORTANCEHIV-1 usurps cellular factors to effectively replicate and avoid innate immune sensing. HIV-1 capsid binds CPSF6, but the consequences of this virus-host interaction on HIV-1 biology are necessarily complicated by the cellular role of CPSF6 in pre-RNA processing. By endogenously recoding CPSF6 to selectively disrupt capsid binding while preserving its RNA-processing activity, we demonstrate in two different T cell lines that CPSF6-mediated targeting of HIV-1 DNA integration into nuclear speckle-associated domains correlates with optimal viral replication. Recoded THP-1 myeloid/macrophage cells revealed additional single-round and multi-round infection defects, which correlated with reductions in reverse transcription and HIV-1 nuclear import. These defects, however, did not correlate with enhanced type I interferon production or detection of HIV-1 nucleic acids by known innate immune sensors. Our work clarifies diverse roles for the capsid-CPSF6 interaction in HIV-1 infection of T lymphocytic versus myeloid cell lines and provides novel cell reagents for future research studies. FAU - Jang, Sooin AU - Jang S AD - Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA. RINGGOLD: 1855. ROR: https://ror.org/02jzgtq86 AD - Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA. RINGGOLD: 1812. ROR: https://ror.org/03vek6s52 FAU - Radhakrishnan, Rajalingam AU - Radhakrishnan R AD - Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA. RINGGOLD: 1855. ROR: https://ror.org/02jzgtq86 FAU - Greenwood, Juliet AU - Greenwood J AD - Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA. RINGGOLD: 1855. ROR: https://ror.org/02jzgtq86 FAU - Singh, Parmit K AU - Singh PK AD - Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA. RINGGOLD: 1855. ROR: https://ror.org/02jzgtq86 AD - Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA. RINGGOLD: 1812. ROR: https://ror.org/03vek6s52 FAU - Bedwell, Gregory J AU - Bedwell GJ AD - Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA. RINGGOLD: 1855. ROR: https://ror.org/02jzgtq86 AD - Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA. RINGGOLD: 1812. ROR: https://ror.org/03vek6s52 FAU - Robles-Sikisaka, Refugio AU - Robles-Sikisaka R AD - Department of Immunology and Microbiology, Scripps Research, La Jolla, California, USA. RINGGOLD: 4356 FAU - Routh, Andrew L AU - Routh AL AUID- ORCID: 0000-0002-2874-5990 AD - Department of Immunology and Microbiology, Scripps Research, La Jolla, California, USA. RINGGOLD: 4356 FAU - Engelman, Alan N AU - Engelman AN AUID- ORCID: 0000-0002-9709-2591 AD - Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA. RINGGOLD: 1855. ROR: https://ror.org/02jzgtq86 AD - Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA. RINGGOLD: 1812. ROR: https://ror.org/03vek6s52 LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - mBio JT - mBio JID - 101519231 SB - IM OTO - NOTNLM OT - CPSF6 OT - HIV-1 OT - capsid OT - innate immunity OT - integration OT - nuclear import OT - replication EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:05 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:05 [entrez] AID - 10.1128/mbio.02276-26 [doi] PST - aheadofprint SO - mBio. 2026 Oct 29:e0227626. doi: 10.1128/mbio.02276-26. PMID- 42954479 OWN - NLM STAT- Publisher LR - 20261029 IS - 2192-5682 (Print) IS - 2192-5682 (Linking) DP - 2026 Oct 29 TI - Anterior-Only Stabilization of Traumatic Subaxial Cervical Spine Injuries: Why Constructs Fail and Why Most Survive-A Systematic Review. PG - 21925682261502382 LID - 10.1177/21925682261502382 [doi] AB - Study DesignSystematic review.ObjectivesTo characterize mechanical failure rates and candidate risk signals for failure after anterior-only stabilization for traumatic subaxial cervical spine injuries.MethodsWe searched MEDLINE, Embase, Scopus, Web of Science, and the Cochrane Central Register of Controlled Trials from inception to June 1, 2026 for studies evaluating mechanical construct failure or candidate risk signals after anterior-only stabilization for subaxial fractures. Narrative synthesis reported crude event fractions and study-specific odds ratios without pooling. Risk of bias and evidence certainty were assessed using ROBINS-I and GRADE (PROSPERO CRD420261420216).ResultsSix studies were included: five retrospective clinical cohorts (659 patients) and one nationwide registry (1,486 patients). Crude failure fractions were 5.8% (38/659) overall and 8.1% (33/405) in a morphology-focused descriptive subset, excluding separable stiff-spine cases. The separately analyzed registry recorded implant failure in 0.9% (14/1,486), implant malposition in 1.0% (15/1,486). Candidate risk signals included posterior tension-band injury (bilateral facet dislocation, posterior longitudinal ligament [PLL] injury, and facet fracture), compression endplate fracture, older age, and C7-T1 involvement; AO Spine Type C morphology was associated with registry implant failure. Compression endplate fracture and older age were each associated with failure in two cohorts; the remaining signals in one. In three cohorts with specific timing, failures were identified within seven postoperative weeks. Evidence certainty was very low.ConclusionsMechanical failure appears uncommon in selected anterior plate-based cohorts. Each candidate risk signal was supported by one or two studies; effects may depend on patient selection and surgical technique. We propose a hypothesis-generating construct-survival framework balancing mechanical demand against construct capacity until fusion and requiring prospective validation. FAU - Alwady, Deya AU - Alwady D AUID- ORCID: 0009-0009-8165-7859 AD - Centre for Spinal Studies and Surgery, Queen's Medical Centre, Nottingham University Hospitals NHS Trust, Nottingham, UK. FAU - Aly, Mohamed M AU - Aly MM AUID- ORCID: 0000-0001-5221-8288 AD - Department of Neurosurgery, Mansoura University, Mansoura, Egypt. AD - Department of Neurosurgery, Prince Mohammed Bin Abdulaziz Hospital, Riyadh, Saudi Arabia. FAU - Bransford, Richard AU - Bransford R AUID- ORCID: 0000-0003-2293-5774 AD - Department of Orthopaedics and Sports Medicine, Harborview Medical Center, University of Washington, Seattle, WA, USA. RINGGOLD: 7284 FAU - Joaquim, Andrei AU - Joaquim A AUID- ORCID: 0000-0003-2645-0483 AD - Neurosurgery Division, Department of Neurology, State University of Campinas (UNICAMP), Campinas-Sao Paulo, Brazil. FAU - Costachescu, Bogdan AU - Costachescu B AD - Department of Neurosurgery, "Prof. Dr. N. Oblu" Emergency Clinical Hospital, Iasi, Romania. AD - Department of Neurosurgery, Grigore T. Popa University of Medicine and Pharmacy, Iasi, Romania. FAU - Popescu, Eugen Cezar AU - Popescu EC AUID- ORCID: 0000-0001-5732-5402 AD - Department of Neurosurgery, "Prof. Dr. N. Oblu" Emergency Clinical Hospital, Iasi, Romania. FAU - Bin Shebreen, Abdulaziz AU - Bin Shebreen A AD - Spine Surgery Department, King Fahad Medical City, Riyadh Second Health Cluster, Riyadh, Saudi Arabia. RINGGOLD: 37849 AD - National Neurosciences Institute, Riyadh, Saudi Arabia. FAU - Baeesa, Saleh AU - Baeesa S AUID- ORCID: 0000-0002-3053-7912 AD - Department of Neurosciences, King Faisal Specialist Hospital and Research Centre, Jeddah, Saudi Arabia. FAU - Bin Dajim, Nayef AU - Bin Dajim N AD - Rameem Medical Company, Saudi Arabia. FAU - Salem, Khalid AU - Salem K AD - Centre for Spinal Studies and Surgery, Queen's Medical Centre, Nottingham University Hospitals NHS Trust, Nottingham, UK. FAU - Riew, K Daniel AU - Riew KD AUID- ORCID: 0000-0003-2083-9375 AD - Department of Neurological Surgery, Weill Cornell Medical Center, New York, USA. RINGGOLD: 189446 AD - Department of Orthopaedic Surgery, Columbia University Medical Center, New York, USA. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - Global Spine J JT - Global spine journal JID - 101596156 OTO - NOTNLM OT - ACDF OT - Anterior-Only Stabilization OT - Cervical Trauma OT - Mechanical Construct Failure OT - Subaxial Cervical Spine Injury EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:04 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:04 [entrez] AID - 10.1177/21925682261502382 [doi] PST - aheadofprint SO - Global Spine J. 2026 Oct 29:21925682261502382. doi: 10.1177/21925682261502382. PMID- 42954473 OWN - NLM STAT- Publisher LR - 20261029 IS - 2379-5042 (Electronic) IS - 2379-5042 (Linking) DP - 2026 Oct 29 TI - SARS-CoV-2 membrane (M) protein activates TLR4 to induce an inflammatory response. PG - e0056126 LID - 10.1128/msphere.00561-26 [doi] AB - An excessive inflammatory response, also known as "cytokine storm," is a major cause of severe COVID-19. Viral RNA and its structural proteins are key mediators of SARS-CoV-2-induced inflammation. While the immune sensors and host inflammatory responses induced by SARS-CoV-2 RNA and the structural proteins spike (S), envelope (E), and nucleocapsid (N) are well defined, the role of membrane (M) protein in inflammatory/antiviral response and the signaling pathway facilitating M protein-mediated immune response is not well characterized. Here, we show that SARS-CoV-2 M protein induces robust inflammatory cytokine responses in murine and human macrophages and lungs. We also show that the M protein-induced inflammatory response relies on TLR4 signaling and that MyD88 serves as the key adaptor driving inflammation, while TRIF is primarily required for the antiviral IFN response. Detailed evaluation of downstream signaling pathways highlighted the critical roles of mitogen-activated protein kinase (MAPK) molecules JNK and ERK1/2, followed by nuclear factor kappa B (NF-kappaB) and p38 signaling in the M protein-TLR4-mediated inflammatory response. Finally, we show that abrogation of M protein-induced ERK1/2 and p38 signaling reduced inflammatory cytokine production and rescued type I interferon production in wild-type macrophages, while inhibition of JNK and NF-kappaB suppressed the M protein-induced inflammatory and antiviral cytokine responses. Collectively, our results demonstrate that SARS-CoV-2 M protein induces pro-inflammatory cytokines and type I interferons via TLR4-MyD88/TRIF activation, with MAPK activation playing a dominant role, and offer specific targeting of MAPK signaling to mitigate SARS-CoV-2-induced excessive inflammation and pathology. IMPORTANCE: Severe COVID-19 is often caused by an excessive inflammatory response, but the role of the SARS-CoV-2 membrane (M) protein in this process has not been well understood. We show that the M protein strongly activates inflammatory and antiviral immune responses through TLR4 signaling and identify the key signaling pathways involved. We also demonstrate that selectively targeting MAPK signaling can reduce harmful inflammation while restoring antiviral interferon responses. These findings improve our understanding of how SARS-CoV-2 causes disease and identify potential therapeutic targets to limit excessive inflammation without compromising antiviral immunity. FAU - Johnson, Paige Marie AU - Johnson PM AUID- ORCID: 0009-0007-7291-8290 AD - Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, Oklahoma, USA. RINGGOLD: 7618. ROR: https://ror.org/01g9vbr38 FAU - Pabbineedi, Sai Mounica AU - Pabbineedi SM AUID- ORCID: 0009-0006-4359-0401 AD - Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, Oklahoma, USA. RINGGOLD: 7618. ROR: https://ror.org/01g9vbr38 FAU - Shrestha, Rakshya AU - Shrestha R AUID- ORCID: 0000-0002-1109-7966 AD - Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, Oklahoma, USA. RINGGOLD: 7618. ROR: https://ror.org/01g9vbr38 FAU - Whitley, Cody J AU - Whitley CJ AUID- ORCID: 0009-0007-5675-701X AD - Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, Oklahoma, USA. RINGGOLD: 7618. ROR: https://ror.org/01g9vbr38 FAU - More, Sunil AU - More S AUID- ORCID: 0000-0001-5007-8795 AD - Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, Oklahoma, USA. RINGGOLD: 7618. ROR: https://ror.org/01g9vbr38 AD - Oklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, Oklahoma, USA. RINGGOLD: 7618. ROR: https://ror.org/01g9vbr38 FAU - Channappanavar, Rudragouda AU - Channappanavar R AUID- ORCID: 0000-0002-4067-278X AD - Department of Veterinary Pathobiology, Oklahoma State University, Stillwater, Oklahoma, USA. RINGGOLD: 7618. ROR: https://ror.org/01g9vbr38 AD - Oklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, Oklahoma, USA. RINGGOLD: 7618. ROR: https://ror.org/01g9vbr38 LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - mSphere JT - mSphere JID - 101674533 SB - IM OTO - NOTNLM OT - SARS-CoV-2 OT - TLR4 OT - cytokines OT - inflammation OT - innate immunity OT - macrophages OT - membrane protein EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:04 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:04 [entrez] AID - 10.1128/msphere.00561-26 [doi] PST - aheadofprint SO - mSphere. 2026 Oct 29:e0056126. doi: 10.1128/msphere.00561-26. PMID- 42954465 OWN - NLM STAT- Publisher LR - 20261029 IS - 2165-0497 (Electronic) IS - 2165-0497 (Linking) DP - 2026 Oct 29 TI - From saccharides to methane: substrate-driven microbial diversity and methanogenesis in anaerobic digestion revealed by 390 MAGs. PG - e0285426 LID - 10.1128/spectrum.02854-26 [doi] AB - Anaerobic digestion (AD) is a key technology for organic waste treatment and renewable energy production, yet its microbial processes remain poorly understood. This study employed genome-centric metagenomics to investigate synthetic AD systems fed with five substrates: starch, glucose, butyrate, propionate-valerate, and acetate. A total of 390 high-quality metagenome-assembled genomes (MAGs) were reconstructed, 64.36% of which represent potentially novel species. Substrate-driven selection shaped distinct functional consortia, with Bifidobacterium enriched in starch hydrolysis and Syntrophomonas enriched in butyrate oxidation. A complete set of acetoclastic methanogenesis-associated genes was identified in a bacterial MAG (Armatimonadota UBA5829). A complete CO(2) reduction pathway was also identified in Methanothrix soehngenii. Glycine cleavage systems were prevalent in high-abundance MAGs, while key Wood-Ljungdahl pathway enzymes were absent. These findings provide genomic insights into substrate-driven assembly and methanogenic pathway distribution in synthetic AD systems. IMPORTANCE: Anaerobic digestion is a key technology for converting organic waste into renewable energy, yet the microbial processes underlying its efficiency remain largely hidden. By reconstructing 390 microbial genomes from digestion systems fed with different sugar-derived substrates, this study unveils how specific substrates shape microbial communities and reveals unexpected metabolic pathways for methane production. These findings challenge long-standing assumptions about which organisms perform key steps in the process, provide a valuable genomic resource for the field, and offer concrete targets for improving waste-to-energy systems through microbiome engineering. FAU - Pan, Xiaoli AU - Pan X AUID- ORCID: 0000-0001-9728-430X AD - Department of Environment and Quality Test, Chongqing Chemical Industry Vocational College, Chongqing, China. RINGGOLD: 654022 AD - Chongqing Key Laboratory of Bioresource for Bioenergy, College of Resources and Environment, Southwest University, Chongqing, China. RINGGOLD: 26463. ROR: https://ror.org/01kj4z117 FAU - Chen, Xugeng AU - Chen X AD - Chongqing SIIE Quality Testing Co., Ltd., Chongqing, China. FAU - Xie, Haiyin AU - Xie H AD - Chongqing Key Laboratory of Bioresource for Bioenergy, College of Resources and Environment, Southwest University, Chongqing, China. RINGGOLD: 26463. ROR: https://ror.org/01kj4z117 FAU - Zhang, Yongjiang AU - Zhang Y AD - Department of Environment and Quality Test, Chongqing Chemical Industry Vocational College, Chongqing, China. RINGGOLD: 654022 FAU - Dai, Yan AU - Dai Y AD - Department of Environment and Quality Test, Chongqing Chemical Industry Vocational College, Chongqing, China. RINGGOLD: 654022 FAU - Yuan, Changguo AU - Yuan C AD - Key Laboratory for Brewing Microorganism Application and Detection Technology, Luzhou Vocational and Technical College, Sichuan, China. ROR: https://ror.org/002hfez23 FAU - Kong, Fanjing AU - Kong F AD - Department of Environment and Quality Test, Chongqing Chemical Industry Vocational College, Chongqing, China. RINGGOLD: 654022 FAU - Luo, Feng AU - Luo F AUID- ORCID: 0000-0001-7510-2675 AD - Chongqing Key Laboratory of Bioresource for Bioenergy, College of Resources and Environment, Southwest University, Chongqing, China. RINGGOLD: 26463. ROR: https://ror.org/01kj4z117 LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Microbiol Spectr JT - Microbiology spectrum JID - 101634614 SB - IM OTO - NOTNLM OT - anaerobic digestion OT - genome-centric metagenomics OT - methanogenesis OT - microbial community OT - substrate-driven selection EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:04 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:04 [entrez] AID - 10.1128/spectrum.02854-26 [doi] PST - aheadofprint SO - Microbiol Spectr. 2026 Oct 29:e0285426. doi: 10.1128/spectrum.02854-26. PMID- 42954461 OWN - NLM STAT- Publisher LR - 20261029 IS - 2165-0497 (Electronic) IS - 2165-0497 (Linking) DP - 2026 Oct 29 TI - Adjunctive recombinant human thymosin beta4 therapy improves antibacterial outcomes against Staphylococcus aureus. PG - e0357025 LID - 10.1128/spectrum.03570-25 [doi] AB - Thymosin beta4 (Tbeta4) is an endogenous multifunctional protein with growing therapeutic applications, including infectious diseases; however, its intrinsic antibacterial activity remains unclear. Previous research with the peptide and related shellfish homologs has suggested broad-spectrum antimicrobial properties, including activity against Staphylococcus aureus. Here, we evaluated the antibacterial activity of recombinant human Tbeta4 (rhTbeta4) alone and in combination with antibiotics against S. aureus strain 8325-4 and two clinical isolates. Direct antibacterial activity of rhTbeta4 (15.625-16,000 microg/mL) was assessed by broth microdilution and colony-forming unit enumeration. Interactions between rhTbeta4 and antibiotics were evaluated using checkerboard assays examining total, planktonic, and biofilm-associated bacterial populations. Biofilms were further analyzed using crystal violet staining and SYTO9/PI labeling to assess biomass and membrane integrity, respectively. Moxifloxacin, ciprofloxacin, oxacillin, and vancomycin were evaluated. rhTbeta4 alone exhibited modest growth-modulating effects but lacked substantial direct antibacterial activity. In contrast, rhTbeta4 demonstrated synergistic interactions with multiple antibiotics, resulting in reduced bacterial growth primarily within total and planktonic populations. Limited effects were observed against established biofilms, as rhTbeta4 did not significantly reduce biofilm biomass or membrane integrity, and combination treatments produced antibiotic-dependent biofilm responses. Collectively, these findings indicate that rhTbeta4 possesses little intrinsic antibacterial activity against S. aureus but can synergistically augment the effects of select antibiotics in vitro, supporting its utility as an adjunctive therapeutic strategy.IMPORTANCEStaphylococcus aureus remains a leading cause of severe infections worldwide, and the rise in antibiotic resistance emphasizes the need for new therapeutic strategies. Thymosin beta4 (Tbeta4), a naturally occurring human peptide already known to promote tissue repair, may also enhance bacterial clearance. This study demonstrates that recombinant human Tbeta4 exhibits direct growth-modulating activity against S. aureus and, more importantly, synergizes with multiple antibiotics to reduce bacterial growth. These findings suggest that Tbeta4 could serve as an adjunctive therapy, improving the efficacy of conventional antibiotics while leveraging its immunomodulatory and wound-healing properties. By uncovering a potential antimicrobial dimension to a well-characterized endogenous peptide, this work broadens the therapeutic scope of Tbeta4 and supports its further development as a novel strategy for treating antibiotic-resistant or biofilm-associated infections. FAU - Banga, Loveleen AU - Banga L AD - Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, Michigan, USA. RINGGOLD: 466086. ROR: https://ror.org/01070mq45 FAU - Patel, Dhara AU - Patel D AD - Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, Michigan, USA. RINGGOLD: 466086. ROR: https://ror.org/01070mq45 FAU - Shawky, Mohamed AU - Shawky M AD - Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, Michigan, USA. RINGGOLD: 466086. ROR: https://ror.org/01070mq45 FAU - Carabulea, Alexander AU - Carabulea A AD - Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, Michigan, USA. RINGGOLD: 466086. ROR: https://ror.org/01070mq45 FAU - Ebrahim, Abdul Shukkur AU - Ebrahim AS AD - Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, Michigan, USA. RINGGOLD: 466086. ROR: https://ror.org/01070mq45 FAU - Liu, Li AU - Liu L AD - Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, Michigan, USA. RINGGOLD: 466086. ROR: https://ror.org/01070mq45 FAU - Berger, Elizabeth AU - Berger E AUID- ORCID: 0000-0001-5371-1170 AD - Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, Michigan, USA. RINGGOLD: 466086. ROR: https://ror.org/01070mq45 LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Microbiol Spectr JT - Microbiology spectrum JID - 101634614 SB - IM OTO - NOTNLM OT - S. aureus OT - Tbeta4 OT - antimicrobial EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:03 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:03 [entrez] AID - 10.1128/spectrum.03570-25 [doi] PST - aheadofprint SO - Microbiol Spectr. 2026 Oct 29:e0357025. doi: 10.1128/spectrum.03570-25. PMID- 42954458 OWN - NLM STAT- Publisher LR - 20261029 IS - 2165-0497 (Electronic) IS - 2165-0497 (Linking) DP - 2026 Oct 29 TI - Slowly burning down the house: the role of calcium in spotted fever rickettsiosis pathogenesis. PG - e0035526 LID - 10.1128/spectrum.00355-26 [doi] AB - Spotted fever group rickettsiae (SFGR) infect microvascular endothelial cells, increasing vascular permeability, a major pathophysiological effect with unclear underlying factors. To accurately model this disease feature, we used a microvascular barrier model that continuously monitors transendothelial electrical resistivity in brain microvascular endothelial cell barriers. Using this model, we previously showed that Rickettsia parkeri-infected microvascular endothelial cells treated with voltage-gated L-, T-, and N-type calcium channel blocker (VGCCb) benidipine delayed in vitro vascular permeability. This study aimed to investigate whether host calcium signaling modulation and metabolic stress with SFGR infection are primary drivers of rickettsial fitness and the subsequent loss of vascular integrity. We hypothesize that SFGR infections, while consuming host cell energy resources such as ATP, lead to modulation of microvascular endothelial cell calcium transport that enhances bacterial fitness, prefacing collateral barrier dysfunction. We confirmed that benidipine, the L-type VGCCb nifedipine, and the membrane-active divalent ion chelator DP-b99 have dose-dependent effects on vascular permeability, as measured by electric cell-substrate impedance sensing, which result in delayed rickettsial growth, as measured by qPCR, without direct bactericidal activity. During R. parkeri infection, intracellular calcium concentrations increased and ATP concentrations decreased simultaneously; these effects were mitigated by benidipine. VGCCb and DP-b99 treatment sustained VE-cadherin junctions, seen with immunofluorescence microscopy, similar to uninfected microvascular endothelial cells, corresponding to barrier integrity preservation. In addition to delayed rickettsial growth, VGCCbs and DP-b99 inhibited rickettsial actin tail formation, reduced cell-to-cell spread, and increased bacterial length by impacting FtsZ-ring localization, evaluated by immunofluorescence microscopy and plaque assay. Furthermore, bacterial protein synthesis is required, as replicating but not heat-killed R. parkeri caused microvascular endothelial cell barrier dysfunction, and a subinhibitory doxycycline dose delayed R. parkeri-induced microvascular endothelial cell dysfunction without impacting R. parkeri growth. Limiting calcium access disrupts R. parkeri growth by interrupting bacterial elongation and division, actin tail formation, and cell-to-cell spread, mitigating R. parkeri-induced microvascular endothelial cell barrier permeability in vitro. This suggests that rickettsial interference with host cell metabolism triggers calcium entry and accessibility, key in rickettsial fitness, and pathogenicity.IMPORTANCEThis work investigated pathogen- and host-associated factors contributing to SFGR disease pathogenesis, as measured by vascular permeability. Our findings demonstrate that SFGR disrupts endothelial cell homeostasis by causing intracellular calcium influx, a process that is critical for rickettsial fitness and pathogenesis. Rickettsial growth and cell-to-cell spread are dependent on host cell calcium, underscoring the essential role of Ca(2)(+) access and linking bacterial fitness to calcium-dependent host processes. Although blocking calcium channels suppresses immune cell activation in vitro and increases mortality in an SFGR murine model in vivo, these findings warrant further investigation into whether selectively targeting endothelial cell-specific calcium signaling could reduce SFGR-associated vascular permeability without compromising innate immune responses. Such an approach may improve SFGR-related morbidity and mortality. Elucidating the role of calcium signaling in the obligate intracellular lifestyle of rickettsiae provides deeper insight into fundamental rickettsial biology and the mechanisms driving severe disease. FAU - Farner, Jennifer M AU - Farner JM AUID- ORCID: 0000-0002-4879-6904 AD - Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, Maryland, USA. RINGGOLD: 44069. ROR: https://ror.org/04v942931 AD - Emerging Infectious Disease Graduate Program, School of Medicine, Uniformed Services University, Bethesda, Maryland, USA. RINGGOLD: 158147. ROR: https://ror.org/04r3kq386 FAU - Nguyen, Trung AU - Nguyen T AD - Department of Molecular Biology and Genetics, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA. RINGGOLD: 189358. ROR: https://ror.org/00za53h95 FAU - Londono, Andres F AU - Londono AF AD - Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, Maryland, USA. RINGGOLD: 44069. ROR: https://ror.org/04v942931 AD - Department of Pathology, School of Medicine, Uniformed Services University, Bethesda, Maryland, USA. RINGGOLD: 732844. ROR: https://ror.org/04r3kq386 FAU - Kim, Yuri AU - Kim Y AD - Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, Maryland, USA. RINGGOLD: 44069. ROR: https://ror.org/04v942931 FAU - Grab, Dennis J AU - Grab DJ AD - Department of Pathology, School of Medicine, Uniformed Services University, Bethesda, Maryland, USA. RINGGOLD: 732844. ROR: https://ror.org/04r3kq386 FAU - Goley, Erin AU - Goley E AUID- ORCID: 0000-0002-8518-2303 AD - Department of Molecular Biology and Genetics, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA. RINGGOLD: 189358. ROR: https://ror.org/00za53h95 FAU - Dumler, J Stephen AU - Dumler JS AUID- ORCID: 0000-0002-8476-1311 AD - Department of Pathology, School of Medicine, Uniformed Services University, Bethesda, Maryland, USA. RINGGOLD: 732844. ROR: https://ror.org/04r3kq386 LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Microbiol Spectr JT - Microbiology spectrum JID - 101634614 SB - IM OTO - NOTNLM OT - Rickettsia OT - Rickettsia parkeri OT - calcium channels OT - host-pathogen interactions OT - host-targeted Therapeutics OT - obligate intracellular bacteria EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:03 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:03 [entrez] AID - 10.1128/spectrum.00355-26 [doi] PST - aheadofprint SO - Microbiol Spectr. 2026 Oct 29:e0035526. doi: 10.1128/spectrum.00355-26. PMID- 42954457 OWN - NLM STAT- Publisher LR - 20261029 IS - 1520-6904 (Electronic) IS - 0022-3263 (Linking) DP - 2026 Oct 29 TI - Total Synthesis, Absolute Configurational Determination, and Antifouling Activity Evaluation of Cretenyne B. LID - 10.1021/acs.joc.6c02249 [doi] AB - The relative configuration of the tetrahydrofuran moiety in cretenyne B (2) has been previously determined. However, the stereochemistries at the C6 and C13 side-chain positions of 2 remain unresolved. Herein, we report the absolute configuration of 2 using nuclear magnetic resonance (NMR) analysis and total synthesis, and the antifouling activity of two candidate diastereomers for 2. To predict the C6 stereochemistry of 2, the natural NMR data were compared with those of the C6 stereoisomers of cretenyne A (1), which we had previously synthesized. This analysis suggested two candidate compounds from four possible diastereomers. We then conducted total synthesis of candidate compounds 2a and 2b. Enone 31 was synthesized using 5-exo-tet-cyclization, cis-selective olefinations, and stereoselective chlorination as key transformations. Luche reduction of 31 served as the branching step at the late-stage of synthesis to yield 2a and 2b. Comparison of their data with those of the natural product revealed natural (-)-cretenyne B as the enantiomer of 2a. We expect 2a and 2b and their synthetic intermediates to be used in the total synthesis of cretenynes C-E (3-5). We also found 2a and 2b to be antifouling-active and nontoxic toward the cypris larvae of the barnacle Amphibalanus amphitrite. CI - (c) 2026 American Chemical Society. FAU - Takamura, Hiroyoshi AU - Takamura H AUID- ORCID: 0000-0002-8330-969X AD - Department of Life Science, Graduate School of Engineering Science, Akita University, 1-1 Tegatagakuen-machi, Akita010-8502, Japan. FAU - Imakiire, Aya AU - Imakiire A AD - Department of Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama700-8530, Japan. FAU - Aso, Ayaka AU - Aso A AD - Department of Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama700-8530, Japan. FAU - Matsunaga, Aya AU - Matsunaga A AD - Department of Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama700-8530, Japan. FAU - Matsuyama, Daiki AU - Matsuyama D AD - Department of Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama700-8530, Japan. FAU - Okuda, Chiharu AU - Okuda C AD - Department of Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama700-8530, Japan. FAU - Roussis, Vassilios AU - Roussis V AUID- ORCID: 0000-0001-9856-4725 AD - Section of Pharmacognosy and Chemistry of Natural Products, Department of Pharmacy, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, Athens15771, Greece. FAU - Tanaka, Kenta AU - Tanaka K AUID- ORCID: 0000-0001-8253-3561 AD - Department of Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama700-8530, Japan. FAU - Kadota, Isao AU - Kadota I AD - Department of Chemistry, Graduate School of Environmental, Life, Natural Science and Technology, Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama700-8530, Japan. LA - eng GR - JP21H01938/Japan Society for the Promotion of Science/ GR - JP23K21115/Japan Society for the Promotion of Science/ GR - JP26K08351/Japan Society for the Promotion of Science/ PT - Journal Article DEP - 20261029 PL - United States TA - J Org Chem JT - The Journal of organic chemistry JID - 2985193R SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:03 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/20 00:00 [revised] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:03 [entrez] AID - 5460653 [pii] AID - 10.1021/acs.joc.6c02249 [doi] PST - aheadofprint SO - J Org Chem. 2026 Oct 29. doi: 10.1021/acs.joc.6c02249. PMID- 42954456 OWN - NLM STAT- Publisher LR - 20261029 IS - 2165-0497 (Electronic) IS - 2165-0497 (Linking) DP - 2026 Oct 29 TI - Development and optimization of self-collected, field-stable, saliva-based immunoassays for scalable epidemiological surveillance of pathogen-specific immunity. PG - e0299626 LID - 10.1128/spectrum.02996-26 [doi] AB - Serological surveillance is fundamental to infectious disease research and informed public-health decision-making. Immunoassays used in the study of pathogen-specific immunity have historically relied on the collection of venous blood. While critical for many public-health applications, this sample collection method is invasive and resource-intensive. The costs and logistical barriers associated with venous blood collection are exacerbated in resource-limited regions, and the shift to less invasive sampling methods would increase sample availability for pathogen surveillance and study of pathogen-specific immunity. To this end, we have developed and optimized a self-collected, saliva-based immunoassay capable of quantifying pathogen-specific antibody binding in saliva samples. Using samples collected from geographically and epidemiologically diverse regions of the world, we compared antigen-specific IgG levels in paired plasma and saliva samples. We observed that levels of IgG against multiple pathogens of public health concern-including SARS-CoV-2 and dengue virus-were highly correlated in plasma and swab-collected saliva. In addition, the decay of maternally derived antibodies in saliva samples collected from infants was readily observed using this immunoassay, demonstrating the assay's sensitivity and potential for use in measuring antibody kinetics. We posit that this assay represents a climate-stable, non-invasive tool that can aid in the surveillance and study of pathogen-specific immunity across a broad range of public-health indications. IMPORTANCE: Traditional serological surveillance relies on venous blood collection, which is invasive, resource-intensive, and particularly burdensome in low-resource settings. To address these limitations, our team has developed and validated a self-collected, saliva-based immunoassay for quantifying pathogen-specific antibody levels. Testing paired plasma and saliva samples from geographically diverse populations, the authors found that IgG levels against SARS-CoV-2 and dengue virus were highly correlated between the two sample types. The assay was sensitive enough to detect the decay of maternally derived antibodies in infant saliva, demonstrating its utility for tracking antibody kinetics over time. The authors conclude that this non-invasive, climate-stable platform offers a practical alternative for broad pathogen surveillance and immune monitoring across a variety of public health contexts. FAU - Bahr, Lauren E AU - Bahr LE AUID- ORCID: 0009-0008-0080-3934 AD - Department of Microbiology and Immunology, SUNY Upstate Medical University, Syracuse, New York, USA. RINGGOLD: 12302. ROR: https://ror.org/040kfrw16 FAU - Lu, Joseph AU - Lu J AD - Global Health Institute, State University of New York Upstate Medical University, Syracuse, New York, USA. RINGGOLD: 12302. ROR: https://ror.org/040kfrw16 FAU - Buddhari, Darunee AU - Buddhari D AD - Department of Virology, Walter Reed Army Institute of Research - Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand. RINGGOLD: 19965. ROR: https://ror.org/023swxh49 FAU - Hunsawong, Taweewun AU - Hunsawong T AUID- ORCID: 0000-0002-0330-6334 AD - Department of Virology, Walter Reed Army Institute of Research - Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand. RINGGOLD: 19965. ROR: https://ror.org/023swxh49 FAU - Rapheal, Erica AU - Rapheal E AD - University of Minnesota School of Public Health, Minneapolis, Minnesota, USA. RINGGOLD: 5635. ROR: https://ror.org/017zqws13 FAU - Greco, Peter AU - Greco P AD - Global Health Institute, State University of New York Upstate Medical University, Syracuse, New York, USA. RINGGOLD: 12302. ROR: https://ror.org/040kfrw16 FAU - Ware, Lisa AU - Ware L AD - Global Health Institute, State University of New York Upstate Medical University, Syracuse, New York, USA. RINGGOLD: 12302. ROR: https://ror.org/040kfrw16 FAU - Klick, Michelle AU - Klick M AD - Global Health Institute, State University of New York Upstate Medical University, Syracuse, New York, USA. RINGGOLD: 12302. ROR: https://ror.org/040kfrw16 FAU - Farmer, Aaron AU - Farmer A AD - Department of Virology, Walter Reed Army Institute of Research - Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand. RINGGOLD: 19965. ROR: https://ror.org/023swxh49 FAU - Middleton, Frank AU - Middleton F AD - Department of Neuroscience and Physiology, Upstate Medical University, Syracuse, New York, USA. FAU - Thomas, Stephen J AU - Thomas SJ AD - Department of Microbiology and Immunology, SUNY Upstate Medical University, Syracuse, New York, USA. RINGGOLD: 12302. ROR: https://ror.org/040kfrw16 AD - Global Health Institute, State University of New York Upstate Medical University, Syracuse, New York, USA. RINGGOLD: 12302. ROR: https://ror.org/040kfrw16 FAU - Anderson, Kathryn AU - Anderson K AD - Department of Microbiology and Immunology, SUNY Upstate Medical University, Syracuse, New York, USA. RINGGOLD: 12302. ROR: https://ror.org/040kfrw16 FAU - Waickman, Adam T AU - Waickman AT AUID- ORCID: 0000-0002-4536-6714 AD - Department of Microbiology and Immunology, SUNY Upstate Medical University, Syracuse, New York, USA. RINGGOLD: 12302. ROR: https://ror.org/040kfrw16 AD - Global Health Institute, State University of New York Upstate Medical University, Syracuse, New York, USA. RINGGOLD: 12302. ROR: https://ror.org/040kfrw16 LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Microbiol Spectr JT - Microbiology spectrum JID - 101634614 SB - IM OTO - NOTNLM OT - DENV OT - dengue virus OT - non-invasive OT - serological surveillance EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 09:03 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 09:03 [entrez] AID - 10.1128/spectrum.02996-26 [doi] PST - aheadofprint SO - Microbiol Spectr. 2026 Oct 29:e0299626. doi: 10.1128/spectrum.02996-26. PMID- 42954455 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1651-226X (Electronic) IS - 0284-186X (Linking) VI - 65 DP - 2026 Oct 29 TI - RET fusion-positive non-small cell lung cancer in Sweden: a nationwide registry study. PG - 875-883 LID - 10.2340/1651-226X.2026.46817 [doi] AB - BACKGROUND: Rearranged during transfection (RET) fusion-positive non-small cell lung cancer (NSCLC) is a rare but clinically meaningful molecular subgroup. RET inhibitors have demonstrated marked clinical activity; however, real-world data on disease characteristics and outcomes before their widespread introduction remain limited. This study characterizes clinical features, treatment patterns, and survival outcomes of RET fusion-positive NSCLC in a nationwide Swedish cohort, with other driver subgroups as descriptive comparisons. METHODS: Patients >/=18 years diagnosed with histologically confirmed NSCLC in 2018-2022, registered in the Swedish National Lung Cancer Registry and with next-generation sequencing results available, were included. Individuals were categorized by oncogenic driver status, including RET, ALK, BRAF, EGFR, KRAS, ROS1, or wild type. Progression-free survival (PFS) and overall survival (OS) from diagnosis were estimated using Kaplan-Meier methods and standardized Cox proportional hazards models adjusted for age, sex, performance status, smoking, stage, and histology. RESULTS: A total of 68 patients had RET fusion-positive NSCLC. The RET cohort was characterized by younger age, a high proportion of never-smokers (59%), and predominantly adenocarcinoma histology (96%). Most had stage IV disease at diagnosis and the highest rate of liver metastases (56%). KIF5B-RET (71%) and CCDC6-RET (19%) were the most frequent fusion partners. Adjusted median OS was 28 months (95% confidence interval [CI]: 13-46) for RET, 39 months (95% CI: 29-52) for ALK, and 17-26 months for the other groups, with overlapping CIs. CONCLUSION: RET fusion-positive patients were typically younger never-smokers with adenocarcinoma and had encouraging OS, despite limited access to selective RET inhibitors during the study period. FAU - Ang Eklund, Ella AU - Ang Eklund E AUID- ORCID: 0000-0002-7962-6836 AD - Department of Oncology, Sahlgrenska University Hospital, Gothenburg, Sweden; Department of Laboratory Medicine, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden. ella.ang@vgregion.se. FAU - Brunnstrom, Hans AU - Brunnstrom H AD - Department of Genetics, Pathology, and Molecular Diagnostics, Skane University Hospital, Lund, Sweden. FAU - Lampa, Erik AU - Lampa E AD - Epistat AB, Uppsala, Sweden. FAU - Gunnarsson, Kerstin AU - Gunnarsson K AD - Department of Oncology, Sahlgrenska University Hospital, Gothenburg, Sweden; Department of Oncology, Institute of Clinical Sciences, University of Gothenburg, Gothenburg, Sweden. FAU - Vikstrom, Anders AU - Vikstrom A AD - Department of Respiratory Medicine, Linkoping University Hospital, Linkoping, Sweden. FAU - Isaksson, Johan AU - Isaksson J AD - Centre of Research and Development Region Gavleborg, Uppsala University, Sweden. FAU - Lamberg, Kristina AU - Lamberg K AD - Department of Medical Sciences, Section of Pulmonology, Uppsala University Hospital, Uppsala, Sweden. FAU - Botling, Johan AU - Botling J AD - Department of Laboratory Medicine, Institute of Biomedicine, University of Gothenburg, Gothenburg; Department of Clinical Pathology, Sahlgrenska University Hospital, Gothenburg, Sweden. FAU - Fagman, Henrik AU - Fagman H AD - Department of Laboratory Medicine, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden; Department of Clinical Pathology, Sahlgrenska University Hospital, Gothenburg, Sweden. FAU - Sayin, Sama I AU - Sayin SI AD - Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Center for Cancer Research, University of Gothenburg, Gothenburg, Sweden; Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg, Gothenburg, Sweden. FAU - Wagenius, Gunnar AU - Wagenius G AD - Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden. FAU - Akyurek, Levent M AU - Akyurek LM AD - Department of Laboratory Medicine, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden; Department of Clinical Pathology, Sahlgrenska University Hospital, Gothenburg, Sweden. FAU - Berglund, Anders AU - Berglund A AD - Epistat AB, Uppsala, Sweden. FAU - Sayin, Volkan I AU - Sayin VI AD - Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Center for Cancer Research, University of Gothenburg, Gothenburg, Sweden; Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg, Gothenburg, Sweden. FAU - Hallqvist, Andreas AU - Hallqvist A AUID- ORCID: 0000-0002-3705-194X AD - Department of Oncology, Sahlgrenska University Hospital, Gothenburg, Sweden; Department of Oncology, Institute of Clinical Sciences, University of Gothenburg, Gothenburg, Sweden. andreas.hallqvist@vgregion.se. LA - eng PT - Journal Article DEP - 20261029 PL - Sweden TA - Acta Oncol JT - Acta oncologica (Stockholm, Sweden) JID - 8709065 RN - EC 2.7.10.1 (Proto-Oncogene Proteins c-ret) RN - EC 2.7.10.1 (RET protein, human) RN - 0 (Oncogene Proteins, Fusion) SB - IM MH - Humans MH - *Carcinoma, Non-Small-Cell Lung/genetics/mortality/pathology/drug therapy/therapy MH - Female MH - Male MH - *Lung Neoplasms/genetics/mortality/pathology/drug therapy/therapy MH - Registries/statistics & numerical data MH - Middle Aged MH - *Proto-Oncogene Proteins c-ret/genetics MH - Sweden/epidemiology MH - Aged MH - *Oncogene Proteins, Fusion/genetics MH - Adult MH - Aged, 80 and over EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 08:53 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:53 [entrez] AID - 10.2340/1651-226X.2026.46817 [doi] PST - epublish SO - Acta Oncol. 2026 Oct 29;65:875-883. doi: 10.2340/1651-226X.2026.46817. PMID- 42954452 OWN - NLM STAT- Publisher LR - 20261029 IS - 1744-7917 (Electronic) IS - 1672-9609 (Linking) DP - 2026 Oct 29 TI - Managing the global invasive pest Drosophila suzukii: Strategies and challenges. LID - 10.1111/1744-7917.70382 [doi] AB - The spotted wing drosophila (Drosophila suzukii) is a globally invasive pest that threatens the production of soft-skinned and stone fruits. Characterized by a serrated ovipositor that enables females to lay eggs in intact ripening fruit, D. suzukii causes direct larval feeding damage and promotes secondary pathogen infections, leading to significant economic losses. Although chemical insecticides remain the dominant control method in commercial systems, their long-term sustainability is increasingly compromised by insecticide resistance and environmental concerns. This review provides a comprehensive synthesis of current management strategies against D. suzukii, covering advances in monitoring techniques, cultural and physical controls, chemical approaches, and biological control utilizing parasitoid wasps, predators, and entomopathogens. We emphasize that the integration of multiple control measures within a systematic integrated pest management (IPM) framework is essential to achieve effective, economically viable, and ecologically sustainable suppression of this pervasive pest. CI - (c) 2026 Institute of Zoology, Chinese Academy of Sciences. FAU - Feng, Ting AU - Feng T AUID- ORCID: 0009-0007-6156-6752 AD - Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China. AD - Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China. FAU - Wang, Haiyang AU - Wang H AD - Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China. AD - Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China. FAU - Wang, Zeying AU - Wang Z AD - Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China. AD - Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China. FAU - Huang, Jianhua AU - Huang J AD - Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China. AD - Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China. FAU - Chen, Jiani AU - Chen J AUID- ORCID: 0000-0003-3161-8519 AD - Zhejiang Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China. AD - Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China. LA - eng GR - National Key R&D Program of China/ GR - National Natural Science Foundation of China/ GR - (LZ26C140002)/Zhejiang Provincial Natural Science Foundation of China/ PT - Journal Article PT - Review DEP - 20261029 PL - Australia TA - Insect Sci JT - Insect science JID - 101266965 SB - IM OTO - NOTNLM OT - Drosophila suzukii OT - biological control OT - chemical control OT - cultural and physical control OT - insecticide resistance EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 08:53 PHST- 2026/09/21 00:00 [revised] PHST- 2026/02/03 00:00 [received] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:53 [entrez] AID - 10.1111/1744-7917.70382 [doi] PST - aheadofprint SO - Insect Sci. 2026 Oct 29. doi: 10.1111/1744-7917.70382. PMID- 42954450 OWN - NLM STAT- Publisher LR - 20261029 IS - 1532-4117 (Electronic) IS - 1093-4529 (Linking) DP - 2026 Oct 29 TI - Effects of gadolinium on oxidative stress and oxidative DNA damage in zebrafish (Danio rerio) at different temperatures. PG - 1-11 LID - 10.1080/10934529.2026.2754783 [doi] AB - Rare earth elements (REEs) are widely used in high-tech applications across industries such as electrical and electronics, automotive, renewable energy, medical and defense. Consequently, lobal demand for REEs is increasing, driven by developing economies, green technology, and R&D. As their usage grows, REE waste discharged into the environment accumulates and pollutes aquatic ecosystems. Climate change-induced temperature increases are known to adversely affect the vital functions of organisms. It is hypothesized that the impact of environmental pollutants is exacerbated by rising temperatures. This study aimed to investigate the effects of Gadolinium (Gd), selected as a representative REE, at concentrations of 0 (control), 10, 50, and 250 mg/L on the model organism Danio rerio under different temperature conditions (22, 27, and 32 degrees C), using biochemical biomarkers and an oxidative DNA damage biomarker. Superoxide dismutase (SOD) and catalase (CAT) levels, glutathione peroxidase (GPx) activity, as well as malondialdehit (MDA) and reduced glutathione (GSH) levels, were measured in whole-body tissue supernatants at 24 and 96 h. Additionally, oxidative DNA damage was evaluated using 8-hydroxy-2'-deoxyguanosine (8-OHdG). Results showed decreased SOD and CAT levels, decreased GPx activity, increased MDA levels, decreased GSH levels, and increased 8-OHdG levels. These findings indicate alterations in antioxidant defense, lipid peroxidation, and oxidative DNA damage following Gd exposure under the tested temperature conditions. FAU - Aydin, Ayse Nur AU - Aydin AN AUID- ORCID: 0000-0002-5657-8958 AD - General Directorate of Agricultural Research and Policies, Aquaculture Central Research Institute, Trabzon, Turkey. FAU - Aydin, Rahmi AU - Aydin R AUID- ORCID: 0000-0002-3002-0892 AD - Munzur University Vocational High School, Tunceli, Turkey. FAU - Serdar, Osman AU - Serdar O AUID- ORCID: 0000-0003-1744-8883 AD - Faculty of Fisheries, Munzur University, Tunceli, Turkey. AD - Faculty of Fisheries, Recep Tayyip Erdogan University, Tunceli, Turkey. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - J Environ Sci Health A Tox Hazard Subst Environ Eng JT - Journal of environmental science and health. Part A, Toxic/hazardous substances & environmental engineering JID - 9812551 SB - IM OTO - NOTNLM OT - Danio rerio OT - biomarker OT - climate change OT - gadolinium OT - oxidative DNA damage OT - rare earth element EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 08:53 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:53 [entrez] AID - 10.1080/10934529.2026.2754783 [doi] PST - aheadofprint SO - J Environ Sci Health A Tox Hazard Subst Environ Eng. 2026 Oct 29:1-11. doi: 10.1080/10934529.2026.2754783. PMID- 42954448 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1473-2300 (Electronic) IS - 0300-0605 (Linking) VI - 54 IP - 10 DP - 2026 Oct TI - Temporal patterns and influencing factors associated with low anterior resection syndrome after rectal cancer surgery: A scoping review. PG - 3000605261487277 LID - 10.1177/03000605261487277 [doi] AB - ObjectivesTo map the existing evidence on the evolution of low anterior resection syndrome, including factors associated with stage-specific low anterior resection syndrome outcomes and longitudinal changes in symptoms.MethodsThis scoping review was conducted in accordance with the Joanna Briggs Institute methodology and reported following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews guideline. Seven databases were searched through 31 December 2025, with English databases searched from 1 January 2012 and Chinese databases from 1 January 2013. Eligible studies included adults aged >/=18 years who underwent sphincter-preserving anterior resection for rectal cancer and were diagnosed with low anterior resection syndrome at least twice postoperatively.ResultsTwenty-three studies were included. Major low anterior resection syndrome was most prevalent during the early follow-up period, particularly at approximately 1-3 months. Symptom improvement was most evident between 6 and 18 months, whereas overall symptom patterns tended to stabilize after approximately 18-24 months, with relatively limited changes subsequently up to 36 months. Tumor and anastomotic location, surgical approach, radiotherapy and/or chemotherapy, and stoma-related factors were associated with low anterior resection syndrome at different follow-up stages or with longitudinal symptom changes. However, only a few studies directly examined longitudinal changes or trajectory-related factors.ConclusionLow anterior resection syndrome is a dynamic condition with stage-specific patterns and substantial inter-individual variability. Understanding its temporal evolution and associated factors may help identify high-risk patients, optimize the timing of assessment, and tailor supportive care and rehabilitation across recovery stages. However, heterogeneity in study design, follow-up schedules, and outcome measures limits comparability. Future research should adopt standardized longitudinal designs, harmonized follow-up protocols, larger samples, and more comprehensive assessment tools to strengthen evidence for individualized low anterior resection syndrome management. FAU - He, Shihui AU - He S AUID- ORCID: 0009-0007-7219-5002 AD - School of Nursing, Beijing University of Chinese Medicine, China. RINGGOLD: 47839 FAU - Chang, Yuan AU - Chang Y AD - School of Nursing, Beijing University of Chinese Medicine, China. RINGGOLD: 47839 FAU - Gao, Linlin AU - Gao L AD - Center for Vascular Surgery and Minimally Invasive Oncology Therapy, Beijing Hospital, China. RINGGOLD: 117555 FAU - Zhao, Fuyun AU - Zhao F AD - School of Nursing, Beijing University of Chinese Medicine, China. RINGGOLD: 47839 FAU - Shi, Jiyuan AU - Shi J AD - School of Nursing, Beijing University of Chinese Medicine, China. RINGGOLD: 47839 FAU - Ma, Jianwen AU - Ma J AD - School of Nursing, Beijing University of Chinese Medicine, China. RINGGOLD: 47839 FAU - Zhang, Changshuo AU - Zhang C AD - School of Nursing, Beijing University of Chinese Medicine, China. RINGGOLD: 47839 FAU - Yue, Shujin AU - Yue S AD - School of Nursing, Beijing University of Chinese Medicine, China. RINGGOLD: 47839 LA - eng PT - Journal Article PT - Review PT - Scoping Review DEP - 20261029 PL - England TA - J Int Med Res JT - The Journal of international medical research JID - 0346411 SB - IM MH - Humans MH - *Low Anterior Resection Syndrome/etiology/epidemiology/diagnosis MH - *Rectal Neoplasms/surgery/pathology MH - *Postoperative Complications/etiology/epidemiology MH - Time Factors MH - Colorectal Surgical Procedures OTO - NOTNLM OT - Low anterior resection syndrome OT - longitudinal follow-up OT - postoperative rehabilitation OT - rectal cancer OT - risk factors OT - scoping review OT - symptom trajectories COIS- Declaration of conflicting interestsThe authors declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 08:52 PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:52 [entrez] AID - 10.1177/03000605261487277 [doi] PST - ppublish SO - J Int Med Res. 2026 Oct;54(10):3000605261487277. doi: 10.1177/03000605261487277. Epub 2026 Oct 29. PMID- 42954437 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1028-768X (Print) IS - 1028-768X (Linking) VI - 35 IP - 4 DP - 2026 Oct 1 TI - Serotonergic Modulation in Absence Epilepsy: The Role of Thalamo-cortical Networks and 5-hydroxytryptamine Receptors. PG - 173-187 LID - 10.4103/ant.ANT-D-26-00044 [doi] AB - Absence epilepsy is characterized by brief nonconvulsive seizures and generalized spike-and-wave discharges (SWDs) generated within distributed cortico-thalamo-cortical networks. Although the roles of T-type calcium channels, GABAergic inhibition, and network synchronization are well established, serotonergic modulation of these circuits remains incompletely defined. This review integrates evidence from genetic and pharmacological animal models, receptor signaling and distribution studies, human neurophysiological and neuroimaging investigations, clinical observations, and emerging receptor-selective approaches. The available evidence indicates that serotonergic effects are determined by receptor subtype, anatomical and cellular localization, developmental stage, and experimental model. Activation of 5-HT2C receptors generally suppresses SWDs, potentially by reducing excessive tonic GABA-A inhibition in thalamocortical neurons, whereas 5-HT7 receptor antagonism reduces SWDs in WAG/Rij rats, although its underlying cellular mechanism remains uncertain. In contrast, 5-HT1A receptor modulation produces bidirectional, model-dependent effects, and thalamic 5-HT2A receptor activation may either suppress or facilitate absence activity depending on the functional state of the network. Evidence concerning 5-HT3, 5-HT4, 5-HT5, and 5-HT6 receptors is sparse. Globally acting serotonergic antidepressants also show heterogeneous effects in preclinical models, and human data do not establish an antiabsence benefit. Preliminary findings from an open-label Phase 2 study of the selective 5-HT2C agonist BMB-101 are insufficient to determine clinical efficacy. Overall, receptor-selective serotonergic mechanisms offer mechanistic insight and potential investigational targets, particularly 5-HT2C and 5-HT7 receptors; however, current evidence does not support altering established absence epilepsy treatment. Controlled clinical studies and developmentally appropriate translational models are required. CI - Copyright (c) 2026 Acta Neurologica Taiwanica. FAU - Aktemur, Burcu Hatipoglu AU - Aktemur BH AD - Department of Physiology, Hamidiye Faculty of Medicine, University of Health Sciences, Istanbul, Turkey. FAU - Saltik, Ozge Darakci AU - Saltik OD AD - Department of Physiology, Faculty of Medicine, Bilecik Seyh Edebali University, Bilecik, Turkey. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - India TA - Acta Neurol Taiwan JT - Acta neurologica Taiwanica JID - 9815355 RN - 0 (Receptors, Serotonin) RN - 333DO1RDJY (Serotonin) SB - IM MH - Humans MH - Animals MH - *Epilepsy, Absence/physiopathology/drug therapy/metabolism MH - *Receptors, Serotonin/physiology MH - *Cerebral Cortex/physiopathology MH - *Thalamus/physiopathology/physiology MH - *Serotonin/physiology/metabolism OTO - NOTNLM OT - 5-hydroxytryptamine receptors OT - absence epilepsy OT - neurophysiology OT - serotonin OT - thalamocortical networks EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 08:43 PHST- 2026/04/22 00:00 [received] PHST- 2026/08/23 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:43 [entrez] AID - 00127883-202610000-00001 [pii] AID - 10.4103/ant.ANT-D-26-00044 [doi] PST - ppublish SO - Acta Neurol Taiwan. 2026 Oct 1;35(4):173-187. doi: 10.4103/ant.ANT-D-26-00044. Epub 2026 Oct 29. PMID- 42954436 OWN - NLM STAT- Publisher LR - 20261029 IS - 1469-0705 (Electronic) IS - 0960-7692 (Linking) DP - 2026 Oct 29 TI - Consensus and persistent uncertainty in fetal ventriculomegaly: Delphi study mapping unresolved clinical domains and research priorities. LID - 10.1002/uog.70370 [doi] AB - OBJECTIVES: Fetal ventriculomegaly (VM) is a common prenatal finding with heterogeneous etiology and unpredictable outcome, yet its management remains among the least standardized in fetal medicine. This study aimed to establish expert consensus on key decision points in the prenatal management of fetal VM, and to systematically map the domains of persistent uncertainty as a structured framework for future research priorities. METHODS: A two-round modified Delphi study was conducted with an international panel of experts in fetal medicine, selected through a systematic review of publications that included more than 50 cases of VM over the preceding decade. Round 1 comprised 39 multiple-choice questions, with consensus achieved when >/= 75% of panelists selected a single answer option; items that did not reach consensus were reformulated into Likert-scale statements for Round 2, which comprised 60 statements rated on a 9-point Likert scale. In Round 2, consensus was defined by two simultaneous criteria: >/= 75% of valid respondents scoring in the agreement (score, 7-9) or disagreement (score, 1-3) zone, with an interquartile range of /=60 years (aOR: 2.49, 95% CI: 1.59-3.91, p < 0.001), and residents of cities with populations of 20 000-499 999 (p < 0.05) were more likely to hold this view. The most common protective measures applied in the last 12 months included keeping windows closed during poor air quality episodes (44.2%) and avoiding time outdoors (36.5%). The imposition of fines for burning prohibited materials in domestic furnaces was supported by 70.2% of participants. Support was more likely among participants aged >/=60 years (aOR: 1.57, 95% CI: 1.03-2.41, p = 0.04), those with higher education (aOR: 1.55, 95% CI: 1.18-2.04, p = 0.002), urban residents (p < 0.05), and those with good household economic status (aOR: 1.53, 95% CI: 1.02-2.29, p = 0.04). CONCLUSIONS: A gap exists between the perception of air pollution as a significant issue and the willingness to apply appropriate mitigation measures. Public knowledge of the effectiveness of air pollution interventions remains insufficient. Int J Occup Med Environ Health. 2026;39(5). CI - This work is available in Open Access model and licensed under a CC BY-NC 3.0 PL license. FAU - Grzesczyk-Nojszewska, Zuzanna AU - Grzesczyk-Nojszewska Z AD - Centre of Postgraduate Medical Education, Warsaw, Poland (School of Public Health). FAU - Sekowski, Kuba AU - Sekowski K AD - Centre of Postgraduate Medical Education, Warsaw, Poland (School of Public Health). FAU - Mazurek, Agnieszka AU - Mazurek A AD - Centre of Postgraduate Medical Education, Warsaw, Poland (School of Public Health). FAU - Winciunas, Piotr AU - Winciunas P AD - Centre of Postgraduate Medical Education, Warsaw, Poland (School of Public Health). FAU - Jankowski, Mateusz AU - Jankowski M AD - Centre of Postgraduate Medical Education, Warsaw, Poland (School of Public Health). FAU - Grudziaz-Sekowska, Justyna AU - Grudziaz-Sekowska J AD - Centre of Postgraduate Medical Education, Warsaw, Poland (School of Public Health). LA - eng PT - Journal Article DEP - 20261028 PL - Poland TA - Int J Occup Med Environ Health JT - International journal of occupational medicine and environmental health JID - 9437093 SB - IM OTO - NOTNLM OT - Poland OT - air pollution OT - attitude OT - environmental hazards preventions OT - perception OT - surveys and questionnaires EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 08:43 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:43 [entrez] AID - 240556 [pii] AID - 10.13075/ijomeh.1896.02831 [doi] PST - aheadofprint SO - Int J Occup Med Environ Health. 2026 Oct 28:240556. doi: 10.13075/ijomeh.1896.02831. PMID- 42954430 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1607-8454 (Electronic) IS - 1024-5332 (Linking) VI - 31 IP - 1 DP - 2026 Dec 31 TI - Daratumumab in immune-mediated thrombotic thrombocytopenic purpura: emerging evidence for plasma cell-targeted therapy. PG - 2753240 LID - 10.1080/16078454.2026.2753240 [doi] AB - INTRODUCTION: Immune mediated thrombotic thrombocytopenic purpura is a life threatening autoimmune thrombotic microangiopathy caused by severe ADAMTS13 deficiency due to inhibitory autoantibodies. Despite plasma exchange, corticosteroids, rituximab, and caplacizumab, some patients develop refractory disease or persistent and relapsing ADAMTS13 deficiency. OBJECTIVES: To summarize the mechanistic rationale, clinical efficacy, safety, and potential therapeutic role of daratumumab in refractory or frequently relapsing immune mediated thrombotic thrombocytopenic purpura. METHODS: A structured narrative review was conducted across five major bibliographic databases, PubMed, EMBASE, the Cochrane Library, China National Knowledge Infrastructure (CNKI), and Web of Science, from database inception through September 13, 2026. Searches of major hematology journal databases were also performed. Case reports, case series, registry analyses, multicenter cohort studies, and conference abstracts describing the use of daratumumab in acquired immune-mediated thrombotic thrombocytopenic purpura (iTTP) were included. Clinical setting, treatment regimen, ADAMTS13 response, time to response, relapse, and adverse events were extracted and qualitatively synthesized. Because reports differ in populations, endpoints, concomitant therapy, and possible patient overlap, findings were synthesized qualitatively without a pooled response estimate. RESULTS: Six key clinical reports involving more than 40 patients were identified. Most patients had received multiple previous therapies, including plasma exchange, corticosteroids, rituximab, and caplacizumab. Daratumumab was administered intravenously at 16 mg/kg or subcutaneously at 1,800 mg, usually weekly for two to six doses. Across the available reports, ADAMTS13 recovery was observed in a substantial proportion of treated patients, although reported response rates varied according to the populations studied and the thresholds used to define response. Recovery commonly occurred within several weeks. Multicenter reports also described sustained remission in a proportion of responders, although biochemical relapse remained frequent during follow-up. Adverse events were generally manageable, with predominantly mild infusion reactions and infrequent serious infections. Other individualized treatment strategies described after rituximab failure or intolerance include bortezomib, optimized or alternative anti-CD20 therapy, ciclosporin, azathioprine, and mycophenolate. CONCLUSION: Daratumumab is a promising plasma cell directed therapy for rituximab refractory or frequently relapsing immune mediated thrombotic thrombocytopenic purpura. Prospective studies are required to define its role within treatment algorithms. FAU - Mohammed Saleh, Mostafa F AU - Mohammed Saleh MF AD - Adult Hematology, Transplantation and Cellular Therapy Department, Oncology Center, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia. AD - Clinical Hematology Unit, Department of Internal Medicine, Faculty of Medicine, Assiut University, Assiut, Egypt. FAU - Alzaidy, Nourah AU - Alzaidy N AD - Adult Hematology, Transplantation and Cellular Therapy Department, Oncology Center, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia. FAU - Nassani, Momen AU - Nassani M AD - Adult Hematology, Transplantation and Cellular Therapy Department, Oncology Center, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia. FAU - Abdrabou, Ahmed Kotb AU - Abdrabou AK AD - Adult Hematology, Transplantation and Cellular Therapy Department, Oncology Center, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia. AD - Clinical Hematology Unit, Internal Medicine Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt. FAU - Nasiri, Abdulrahman AU - Nasiri A AUID- ORCID: 0000-0002-9401-810X AD - College of Medicine, Imam Mohammad Ibn Saud Islamic University, Riyadh, Saudi Arabia. FAU - Alzahrani, Hazza AU - Alzahrani H AD - Adult Hematology, Transplantation and Cellular Therapy Department, Oncology Center, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - Hematology JT - Hematology (Amsterdam, Netherlands) JID - 9708388 RN - 0 (Antibodies, Monoclonal) RN - 4Z63YK6E0E (daratumumab) RN - EC 3.4.24.87 (ADAMTS13 Protein) RN - EC 3.4.24.87 (ADAMTS13 protein, human) SB - IM MH - Humans MH - *Purpura, Thrombotic Thrombocytopenic/drug therapy/immunology MH - *Antibodies, Monoclonal/therapeutic use/pharmacology MH - *Plasma Cells/drug effects/metabolism MH - ADAMTS13 Protein/deficiency MH - *Purpura, Thrombocytopenic, Idiopathic/drug therapy OAB - Immune thrombotic thrombocytopenic purpura, also called iTTP, is a rare and life-threatening disease in which antibodies interfere with ADAMTS13, an enzyme that helps prevent small-vessel clotting. Standard treatment includes plasma exchange, corticosteroids, rituximab, and, in many settings, caplacizumab. Some people remain at high risk because ADAMTS13 activity stays low or falls again despite these treatments. Daratumumab is an antibody that targets CD38-expressing cells, including plasma-cell populations. It is approved for plasma-cell malignancies, not for iTTP. Published experience in iTTP consists of individual reports, small case series, national surveys, a retrospective Swiss cohort, and one international conference abstract. Several reports describe increases in ADAMTS13 activity after daratumumab, but the reports use different definitions, include patients receiving multiple concomitant therapies, and may include some of the same patients. Therefore, the currently available evidence cannot establish a pooled response rate or prove that daratumumab itself caused the observed outcomes. This updated review presents each report separately, incorporates the 2026 full Swiss cohort publication, and explains standard definitions for clinical and ADAMTS13 outcomes. It also discusses bortezomib and other options used after rituximab failure or intolerance. Daratumumab may be considered on an individualized, off-label basis in selected difficult-to-treat cases, ideally with expert multidisciplinary input, infection-risk assessment, and close clinical and laboratory monitoring. Prospective comparative studies are required before its place in routine iTTP care can be defined. OABL- eng OTO - NOTNLM OT - ADAMTS13 OT - daratumumab OT - iTTP OT - immune-mediated thrombotic thrombocytopenic purpura OT - refractory TTP EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 08:43 PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:43 [entrez] AID - 10.1080/16078454.2026.2753240 [doi] PST - ppublish SO - Hematology. 2026 Dec 31;31(1):2753240. doi: 10.1080/16078454.2026.2753240. Epub 2026 Oct 29. PMID- 42954422 OWN - NLM STAT- Publisher LR - 20261029 IS - 1464-0694 (Electronic) IS - 0960-2011 (Linking) DP - 2026 Oct 29 TI - Italian guidelines for speech and language therapy in patients with stroke or primary progressive aphasia. Recommendations from the Italian neuropsychological society. PG - 1-49 LID - 10.1080/09602011.2026.2749403 [doi] AB - BACKGROUND: Aphasia can be frequently observed after left hemisphere stroke, but also in neurodegenerative diseases, as in primary progressive aphasia (PPA). Aphasia strongly impacts on quality of life, and rehabilitation of People with Aphasia (PWA) presents clinicians with complex challenges. AIMS: An ad-hoc Scientific Panel, including 20 members (neurologists, physiatrists, psychologists, therapists, nurses, PWA representatives), selected 4 questions not addressed by current international guidelines: (1) effectiveness of SLT for post-stroke aphasia provided by professionals vs non-specialized personnel; (2) effectiveness of SLT in the chronic phase vs no treatment; (3) effectiveness of non-invasive neuromodulation combined with SLT vs stand-alone SLT for PWA after stroke; (4) effectiveness of non-invasive neuromodulation combined with SLT vs stand-alone SLT for PPA patients. METHODS: The Scientific Panel and experienced methodologists followed common guidelines for systematic reviews and the PICO framework for scrutinizing several databases (last search: March 2023). Recommendations were based on evidence analysis according to the GRADE methodology. KEY FINDINGS: The Scientific Panel suggested one strong and three conditional recommendations, although quality of evidence was low or very low. CONCLUSIONS: The present guidelines, published by the Italian Higher Institute for Health on December 19, 2023, supplement the most recent international guidelines, particularly in tackling PPA. FAU - Papagno, Costanza AU - Papagno C AD - CIMeC, Universita di Trento, Rovereto, Italy. FAU - Cappa, Stefano AU - Cappa S AD - Istituto Superiore di Studi Universitari, Pavia, Italy. FAU - Cruciani, Fabio AU - Cruciani F AD - Dipartimento di Epidemiologia del Servizio Sanitario Regionale del Lazio, Roma, Italy. FAU - Difonzo, Teresa AU - Difonzo T AD - Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, Milano, Italy. FAU - Ferroni, Lucia AU - Ferroni L AD - Physical and Rehabilitative Medicine Unit, Az. USL Toscana Nordovest, Pisa, Italy. FAU - Miceli, Gabriele AU - Miceli G AD - Centro Interdipartimentale Mente/Cervello, Universita di Trento, Center for Mind/Brain Sciences, Rovereto, Italy. FAU - Mitrova, Zuzana AU - Mitrova Z AD - Dipartimento di Epidemiologia del Servizio Sanitario Regionale del Lazio, Roma, Italy. FAU - Quaranta, Davide AU - Quaranta D AD - Universita Cattolica del Sacro Cuore - Campus di Roma, Rome, Italy. FAU - Romani, Cristina AU - Romani C AD - Aston University, Birmingham, UK. FAU - Saulle, Rosella AU - Saulle R AD - Dipartimento di Epidemiologia del Servizio Sanitario Regionale del Lazio, Roma, Italy. FAU - Soda, Mirco AU - Soda M AD - Department of Psychology, Hospital of Bolzano-Bozen (SABES-ASDAA), Bolzano-Bozen, Italy. FAU - Vecchi, Simona AU - Vecchi S AD - Dipartimento di Epidemiologia del Servizio Sanitario Regionale del Lazio, Roma, Italy. FAU - Vigano, Mauro AU - Vigano M AUID- ORCID: 0000-0003-3447-4792 AD - 3UMR 7023 Structures Formelles du Langage, CNRS & Universite Paris 8, Paris, France. FAU - Trojano, Luigi AU - Trojano L AD - Department of Psychology, Universita della Campania "Luigi Vanvitelli", Caserta, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - Neuropsychol Rehabil JT - Neuropsychological rehabilitation JID - 9112672 SB - IM OTO - NOTNLM OT - Aphasia OT - neuromodulation OT - speech and language therapy EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 08:33 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:33 [entrez] AID - 10.1080/09602011.2026.2749403 [doi] PST - aheadofprint SO - Neuropsychol Rehabil. 2026 Oct 29:1-49. doi: 10.1080/09602011.2026.2749403. PMID- 42954417 OWN - NLM STAT- Publisher LR - 20261029 IS - 1097-0061 (Electronic) IS - 0749-503X (Linking) DP - 2026 Oct 29 TI - Spatial Gene Expression in Fungi: mRNA Localization, Condensates, and Beyond. LID - 10.1002/yea.70050 [doi] AB - Polarized growth and cellular asymmetry are broadly conserved properties of fungal life, from transient bud-site selection in unicellular yeasts to sustained hyphal extension in filamentous and coenocytic species. While vesicle trafficking, cytoskeletal organization, and cell-wall remodeling are well-established contributors to these processes, the role of mRNA localization is only beginning to be fully appreciated. In this review, we examine how the spatial regulation of mRNAs and their translation contributes to fungal polarity, morphogenesis, and environmental adaptation. We discuss current knowledge around three interconnected layers contributing to mRNA localization: active cytoskeletal transport-actin-based in compact yeasts and microtubule-based in long hyphae; partitioning into biomolecular condensates such as P-bodies and Whi3-mRNA assemblies; and cytoplasmic-scale architectural features including bulk flow, nuclear positioning, and nuclear heterogeneity that become increasingly important in multinucleate and coenocytic systems such as arbuscular mycorrhizal fungi. Together, these mechanisms establish spatial gene expression as an integral component of fungal polarity, with implications for fungal development, host-pathogen interactions, and ecological adaptation, and for our broader understanding of polarized growth in eukaryotic cell organization. CI - (c) 2026 The Author(s). Yeast published by John Wiley & Sons Ltd. FAU - Otterdijk, Sander van AU - Otterdijk SV AUID- ORCID: 0000-0002-5503-1627 AD - Systems Biology Section, A-LIFE Department, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Vrije Universiteit Amsterdam, Amsterdam, the Netherlands. FAU - Wang, Zixu AU - Wang Z AUID- ORCID: 0000-0002-1879-0691 AD - Systems Biology Section, A-LIFE Department, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Vrije Universiteit Amsterdam, Amsterdam, the Netherlands. FAU - Arends, Roan AU - Arends R AUID- ORCID: 0009-0004-0757-0038 AD - Systems Biology Section, A-LIFE Department, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Vrije Universiteit Amsterdam, Amsterdam, the Netherlands. FAU - Tutucci, Evelina AU - Tutucci E AUID- ORCID: 0000-0002-1998-7146 AD - Systems Biology Section, A-LIFE Department, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Vrije Universiteit Amsterdam, Amsterdam, the Netherlands. LA - eng GR - Vrije Universiteit Amsterdam/ GR - OCENW.M.21.011/NWO_/Dutch Research Council/Netherlands GR - OCENW.M.23.017/NWO_/Dutch Research Council/Netherlands PT - Journal Article PT - Review DEP - 20261029 PL - England TA - Yeast JT - Yeast (Chichester, England) JID - 8607637 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 08:33 PHST- 2026/09/11 00:00 [revised] PHST- 2026/05/23 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:33 [entrez] AID - 10.1002/yea.70050 [doi] PST - aheadofprint SO - Yeast. 2026 Oct 29. doi: 10.1002/yea.70050. PMID- 42954416 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 0869-866X (Print) IS - 0869-866X (Linking) VI - 34 IP - 5 DP - 2026 Dec 15 TI - [The application of geographical informational systems to monitor population health: the review]. PG - 1230-1235 LID - 10.32687/0869-866X-2026-34-5-1230-1235 [doi] AB - The article presents analysis of international experience in practical application of geographic information systems in monitoring of population health on the basis of publications data. The articles were selected from databases of PubMed (Medline included), Google Scholar, eLIBRARY.RU, CyberLeninka and from information portals as of February 2026. The descriptive information about geographic information systems in force applied to implement epidemiological surveillance are obtained from official internet-portals of corresponding agencies.The article presents classification of methodological approaches of applying geographic information systems systems in health care by key tasks according their evolutionary development from description and visualization to complex analytical modeling and evaluation. The experience of application of geographic information systems in epidemiological surveillance systems in the countries that achieved the greatest success in this area, as well as projects that are implementing in Russia is described.Methodologically, geographic information systems in health care evolved from passive cartography to powerful spatial analytical systems with integrated GeoAI that clears the way to precision prevention through identification of clusters, risk assessment and prognostic modeling. The international experience demonstrates that the key to efficient application of geographic information systems for monitoring of morbidity is in standardization of data used or in centralization of platform. In Russia, despite methodological groundwork, geographic information systems has not yet turned out into systemic tool of decision-making to decrease burden of non-communicable diseases and to overcome territorial health inequalities. FAU - Mazilov, S I AU - Mazilov SI AD - The Saratov Hygiene Medical Research Center of the Federal Budget Scientific Institution "Federal Scientific Center for Medical and Preventive Health Risk Management Technologies", 410022, Saratov, Russia. FAU - Osipov, N O AU - Osipov NO AD - The Saratov Hygiene Medical Research Center of the Federal Budget Scientific Institution "Federal Scientific Center for Medical and Preventive Health Risk Management Technologies", 410022, Saratov, Russia. FAU - Komleva, N E AU - Komleva NE AD - The Saratov Hygiene Medical Research Center of the Federal Budget Scientific Institution "Federal Scientific Center for Medical and Preventive Health Risk Management Technologies", 410022, Saratov, Russia. FAU - Pozdnyakov, M V AU - Pozdnyakov MV AD - The Saratov Hygiene Medical Research Center of the Federal Budget Scientific Institution "Federal Scientific Center for Medical and Preventive Health Risk Management Technologies", 410022, Saratov, Russia. FAU - Chekhonatskiy, A A AU - Chekhonatskiy AA AD - The Federal State Budgetary Educational Institution of Higher Education "The V. I. Razumovsky Saratov State Medical University" of Minzdrav of Russia, 410012, Saratov, Russia. LA - rus PT - English Abstract PT - Journal Article PT - Review DEP - 20261215 PL - Russia (Federation) TA - Probl Sotsialnoi Gig Zdravookhranenniiai Istor Med JT - Problemy sotsial'noi gigieny, zdravookhraneniia i istorii meditsiny JID - 101270373 SB - IM MH - Humans MH - *Geographic Information Systems MH - Russia/epidemiology MH - *Population Health MH - *Population Surveillance/methods OTO - NOTNLM OT - geographic information systems OT - non-communicable diseases OT - population health OT - review. OT - social hygienic monitoring EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 08:33 PHST- 2026/10/27 00:00 [received] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:33 [entrez] AID - 10.32687/0869-866X-2026-34-5-1230-1235 [doi] PST - epublish SO - Probl Sotsialnoi Gig Zdravookhranenniiai Istor Med. 2026 Dec 15;34(5):1230-1235. doi: 10.32687/0869-866X-2026-34-5-1230-1235. PMID- 42954415 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1438-8871 (Electronic) IS - 1438-8871 (Linking) VI - 28 DP - 2026 Oct 28 TI - Large Language Model-Assisted Radiology Report Quality Assurance: Real-World Multicenter Evaluation. PG - e106250 LID - 10.2196/106250 [doi] AB - BACKGROUND: Radiology reports serve as the definitive clinical record of imaging diagnoses, with their accuracy being directly critical to patient safety. However, amid escalating workloads, manual review remains inadequate for capturing subtle errors in reports on a large scale. Although large language models (LLMs) have demonstrated considerable promise in medical text processing, previous studies have predominantly been confined to radiology report text from a single imaging modality or datasets with synthetic errors. OBJECTIVE: This study aimed to evaluate the feasibility, accuracy, and generalizability of general-purpose LLMs for quality assurance of radiology report text from multiple imaging modalities (x-ray, computed tomography [CT], and magnetic resonance imaging [MRI]), multi-anatomical regions, and multicenter clinical settings, with head-to-head comparisons conducted against radiologists of varying seniority. METHODS: Radiology report texts from 3 aforementioned imaging modalities and 5 anatomical regions (head, chest, abdomen, spine, and knee) were retrospectively collected from center 1 (March 2025-May 2025) and reviewed using a clinician-annotated 5-category error taxonomy (type 1: interpretive; type 2: factual). Five LLMs (Qwen3, GPT-5.4 Pro, Gemini 3.1 Pro, Llama 4, and DeepSeek-V3.1) were benchmarked on 10,000 randomly selected reports, and the best-performing model was evaluated on the full cohort. A blinded head-to-head comparison with 3 radiologists (3-10 years of experience) used a modality-stratified, error-enriched challenge set. The radiologists subsequently rated the model-generated explanations using a 5-point Likert scale. Cross-center performance was evaluated in an independent external cohort. The same 2 senior radiologists labeled reports from both centers using the same protocol, resolving disagreements by consensus. RESULTS: In total, 4.6% (4431/95,582) of the reports in center 1 contained at least one confirmed text internal error. Qwen3, the selected model, achieved a report-level sensitivity, specificity, positive predictive value, and F(1)-score of 0.99, 0.99, 0.90, and 0.94, respectively, with high performance across reports from the 3 imaging modalities and multiple anatomical regions and better detection of factual than interpretive errors. In the error-enriched challenge set, its error instance exact category recall exceeded that of the radiologists (0.96 vs 0.45-0.67; P<.001), with a shorter mean task completion time (5.80 vs 55.80-67.80 seconds per report). Explanations had a mean Likert score of 4.57, and all inter- and intraobserver intraclass correlation coefficients exceeded 0.89. In center 2, balanced accuracy was 0.94 (95% CI 0.92-0.95). Before consensus, Cohen kappa for report-level error presence was 0.783 (center 1) and 0.850 (center 2). CONCLUSIONS: This study suggests that LLMs can serve as efficient adjunctive tools for text-level quality control of radiology reports, particularly for detecting explicit factual errors. Performance remained stable across centers. In the text-only review task, the model achieved higher exact category recall and shorter task completion times than the participating radiologists. These findings support the potential value of LLM-assisted radiology report text review in real-world clinical environments. CI - (c) Lujing Wang, Yushuang Zhang, Liang Peng, Pei Liu, Li Wang, Yu Cui, Yixin Chen, Xingyu Li, Yitian Tao, Xiuxian Gong, Xiaoying Zhao, Yujiao Song, Jian Chen, Shihua Zhao, Fuying Wang, Jian Ma, Xinxiang Zhao. Originally published in the Journal of Medical Internet Research (https://www.jmir.org). FAU - Wang, Lujing AU - Wang L AUID- ORCID: 0000-0003-0159-7745 AD - Department of Radiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, China, 86 0871-63402918. FAU - Zhang, Yushuang AU - Zhang Y AUID- ORCID: 0009-0007-2273-7387 AD - Department of Radiology, The Sixth Affiliated Hospital of Kunming Medical University, Yuxi, Yunnan, China. FAU - Peng, Liang AU - Peng L AUID- ORCID: 0000-0002-9831-2787 AD - School of Information and Software Engineering, University of Electronic Science and Technology of China, Chengdu, Sichuan, China. FAU - Liu, Pei AU - Liu P AUID- ORCID: 0009-0008-9697-9642 AD - Department of Radiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, China, 86 0871-63402918. FAU - Wang, Li AU - Wang L AUID- ORCID: 0009-0004-3554-4718 AD - Department of Radiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, China, 86 0871-63402918. FAU - Cui, Yu AU - Cui Y AUID- ORCID: 0009-0001-4939-451X AD - Department of Radiology, The Sixth Affiliated Hospital of Kunming Medical University, Yuxi, Yunnan, China. FAU - Chen, Yixin AU - Chen Y AUID- ORCID: 0009-0006-7472-7386 AD - Department of Radiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, China, 86 0871-63402918. FAU - Li, Xingyu AU - Li X AUID- ORCID: 0009-0008-4382-4647 AD - Department of Radiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, China, 86 0871-63402918. FAU - Tao, Yitian AU - Tao Y AUID- ORCID: 0000-0002-1973-5360 AD - School of Biomedical Engineering, ShanghaiTech University, Shanghai, China. FAU - Gong, Xiuxian AU - Gong X AUID- ORCID: 0009-0008-9653-466X AD - Department of Radiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, China, 86 0871-63402918. FAU - Zhao, Xiaoying AU - Zhao X AUID- ORCID: 0000-0003-4116-2105 AD - Department of Radiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, China, 86 0871-63402918. FAU - Song, Yujiao AU - Song Y AUID- ORCID: 0009-0009-4431-2579 AD - Department of Radiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, China, 86 0871-63402918. FAU - Chen, Jian AU - Chen J AUID- ORCID: 0009-0006-2116-6685 AD - Department of Radiology, School of Clinical Medicine, University of Cambridge, Cambridge, United Kingdom. FAU - Zhao, Shihua AU - Zhao S AUID- ORCID: 0000-0003-1339-7476 AD - Department of Radiology, National Center for Cardiovascular Diseases, Fuwai Hospital, Beijing, China. FAU - Wang, Fuying AU - Wang F AUID- ORCID: 0000-0002-3313-6479 AD - Department of Radiology, Stanford Center for Artificial Intelligence in Medicine and Imaging, Stanford University, Stanford, CA, United States. FAU - Ma, Jian AU - Ma J AUID- ORCID: 0009-0007-3680-2194 AD - Department of Radiology, The Sixth Affiliated Hospital of Kunming Medical University, Yuxi, Yunnan, China. FAU - Zhao, Xinxiang AU - Zhao X AUID- ORCID: 0009-0003-9014-8278 AD - Department of Radiology, The Second Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, 650101, China, 86 0871-63402918. LA - eng PT - Journal Article PT - Multicenter Study DEP - 20261028 PL - Canada TA - J Med Internet Res JT - Journal of medical Internet research JID - 100959882 SB - IM MH - *Large Language Models MH - Humans MH - *Quality Assurance, Health Care MH - *Radiology MH - Retrospective Studies MH - Tomography, X-Ray Computed MH - Magnetic Resonance Imaging OTO - NOTNLM OT - AI OT - artificial intelligence OT - clinical AI OT - external validation OT - human evaluation OT - large language model OT - quality assurance OT - radiology report OT - report error EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 08:32 PHST- 2026/07/06 00:00 [received] PHST- 2026/08/24 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:32 [entrez] AID - v28i1e106250 [pii] AID - 10.2196/106250 [doi] PST - epublish SO - J Med Internet Res. 2026 Oct 28;28:e106250. doi: 10.2196/106250. PMID- 42954404 OWN - NLM STAT- Publisher LR - 20261029 IS - 1532-2297 (Electronic) IS - 1082-6068 (Linking) DP - 2026 Oct 29 TI - Enhanced selectivity nanocellulose-based membranes for tangential flow filtration protein separation. PG - 1-8 LID - 10.1080/10826068.2026.2749456 [doi] AB - The development of fully water-based membrane manufacturing approaches represents an important direction toward more sustainable filtration technologies in bioprocessing. The aim of this work was to demonstrate a facile membrane impregnation method for tailoring pore size distribution and achieving enhanced selectivity of protein separation using nanocellulose-based membranes. A series of graded nanocellulose-based membranes was prepared by poly(vinyl alcohol) (PVA) impregnation at varying concentrations (0, 0.5, 1, and 3% wt solution) to produce membranes with tunable size-exclusion characteristics. The membrane structural characteristics were assessed using thermoporometry by differential scanning calorimetry, hydraulic permeability, and Zeman-Wales particle rejection model. The retention of protein samples of varying molecular weight, i.e., 14 kDa (lysozyme), 66 kDa (human serum albumin), and 660 kDa (porcine thyroglobulin), was assessed by ultrafiltration in tangential flow filtration mode. The selectivity parameter, expressed as the ratio between sieving coefficients for a specific pair of proteins (i.e., alpha-factor), increased substantially with impregnation percentage, especially for larger protein pairs, in a controlled manner. It is concluded that precise separation between protein pairs over wide molecular weight ranges can be achieved using nanocellulose-based membranes by considering protein specific MW and selecting an appropriate PVA concentration. FAU - Fomichova, Yuliia AU - Fomichova Y AD - Department of Pharmaceutical Biosciences, Biomedical Center, Uppsala University, Uppsala, Sweden. FAU - Mihranyan, Albert AU - Mihranyan A AD - Department of Pharmaceutical Biosciences, Biomedical Center, Uppsala University, Uppsala, Sweden. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Prep Biochem Biotechnol JT - Preparative biochemistry & biotechnology JID - 9607037 SB - IM OTO - NOTNLM OT - Cross-Flow Filtration OT - Filter Paper OT - Protein Purification OT - Size-Exclusion OT - Ultrafiltration EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 08:23 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:23 [entrez] AID - 10.1080/10826068.2026.2749456 [doi] PST - aheadofprint SO - Prep Biochem Biotechnol. 2026 Oct 29:1-8. doi: 10.1080/10826068.2026.2749456. PMID- 42954402 OWN - NLM STAT- Publisher LR - 20261029 IS - 1944-7876 (Electronic) IS - 1071-1007 (Linking) DP - 2026 Oct 29 TI - Transmalleolar vs Portal-Based Minimally Invasive Tibiotalar Joint Preparation: A Cadaveric Study. PG - 10711007261496281 LID - 10.1177/10711007261496281 [doi] AB - BACKGROUND: Successful ankle arthrodesis depends on thorough preparation of the tibiotalar joint. Open, arthroscopic, and minimally invasive (MIS) techniques exist. MIS burr preparation under fluoroscopy enables smaller incisions and preserves the osteogenic bone slurry. Traditional anteromedial and anterolateral portal-based MIS approaches may leave posterior "blind spots" and limit deformity correction. A transmalleolar MIS technique has been proposed to improve joint access while facilitating apposition and deformity correction. The purpose of this study was to compare the proportion of tibiotalar joint prepared and safety profiles between the transmalleolar and portal-based MIS techniques. METHODS: Fourteen fresh frozen cadaveric lower extremities (7 matched pairs) were randomized to transmalleolar or portal-based MIS tibiotalar joint preparation (n = 7 each). A single fellowship-trained surgeon performed all procedures using standardized burr instrumentation and fluoroscopy. Specimens were disarticulated, photographed with calibration markers, and analyzed using ImageJ to quantify percent preparation of the tibial plafond, talar dome, and combined surfaces. Two independent reviewers (1 masked) performed measurements. Distances to the posterior tibial tendon, posterior tibial artery, and tibial nerve were measured; <5 mm was considered at risk. Paired t tests, Fisher exact test, and ICC were used (P < .05). RESULTS: Transmalleolar MIS achieved greater tibial plafond preparation (94.7% vs 76.8%, P = .009) than the portal technique. Talar dome (67.5% vs 73.5%, P = .534) and combined preparation (83.6% vs 75.5%, P = .248) were similar. Posterior talar zones remained incompletely prepared in most specimens (5/7 vs 6/7). Reliability was excellent (intraclass correlation coefficients >0.90). No neurovascular injuries occurred. Fewer transmalleolar specimens were at risk (28.6% vs 57.1%, P = .592), although not statistically significant. CONCLUSION: Compared with traditional portal-based MIS tibiotalar joint preparation, the transmalleolar MIS technique provided a statistically increased area of tibial plafond preparation without increasing the neurovascular risk. Overall preparation and talar debridement were similar, with comparable safety profiles. LEVEL OF EVIDENCE: Level V, cadaveric / basic science study. FAU - Patel, Arsh N AU - Patel AN AUID- ORCID: 0000-0001-7885-9649 AD - Department of Orthopaedic Surgery, Brooke Army Medical Center, San Antonio, TX, USA. FAU - Durudogan, Eric AU - Durudogan E AUID- ORCID: 0009-0003-4030-3858 AD - Department of Foot and Ankle Surgery, Northwestern University, Evanston, IL, USA. FAU - Arave, Mariah AU - Arave M AUID- ORCID: 0000-0002-8142-6622 AD - Department of Orthopaedic Surgery, Brooke Army Medical Center, San Antonio, TX, USA. FAU - Harish, Murari AU - Harish M AD - Department of Research, Kansas City University School of Osteopathic Medicine, Kansas City, MO, USA. FAU - Achay, Jennifer AU - Achay J AD - Centre for Health Sciences, Bulverde, TX, USA. FAU - Huh, Jeannie AU - Huh J AUID- ORCID: 0000-0002-8229-5767 AD - Department of Orthopaedic Surgery, Brooke Army Medical Center, San Antonio, TX, USA. FAU - Kadakia, Anish AU - Kadakia A AD - Department of Foot and Ankle Surgery, Northwestern University, Evanston, IL, USA. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Foot Ankle Int JT - Foot & ankle international JID - 9433869 SB - IM OTO - NOTNLM OT - ankle arthrodesis OT - arthritis OT - minimally invasive OT - tibiotalar joint EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 08:22 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:22 [entrez] AID - 10.1177/10711007261496281 [doi] PST - aheadofprint SO - Foot Ankle Int. 2026 Oct 29:10711007261496281. doi: 10.1177/10711007261496281. PMID- 42954395 OWN - NLM STAT- Publisher LR - 20261029 IS - 1365-2672 (Electronic) IS - 1364-5072 (Linking) DP - 2026 Oct 29 TI - Drug Repurposing and Advanced Delivery Systems Targeting Multidrug-Resistant Microorganisms: A Narrative Review. LID - lxag268 [pii] LID - 10.1093/jambio/lxag268 [doi] AB - Antimicrobial resistance (AMR) is a major global health threat, driven by the spread of multidrug-resistant (MDR) pathogens alongside a declining antibiotic pipeline. Drug repurposing offers a cost-effective, time-efficient alternative by identifying antimicrobial applications for approved drugs with established safety profiles. Agents including auranofin, niclosamide, gallium compounds, phenothiazines, selective serotonin reuptake inhibitors, ticagrelor, and dronedarone show antimicrobial, antibiofilm, antivirulence, and resistance-modifying activity through mechanisms distinct from conventional antibiotics, potentially limiting cross-resistance. However, reported in vitro minimum inhibitory concentrations (MICs) frequently exceed clinical breakpoints and plasma concentrations achievable at approved doses, confining most candidates to adjuvant or local therapy rather than systemic monotherapy. Poor solubility, limited bioavailability, unfavorable pharmacokinetics, and systemic toxicity further constrain clinical translation. Advanced drug delivery systems (DDS) like nanoparticles, liposomes, metal-organic frameworks, and stimuli-responsive carriers may mitigate these barriers by improving stability, targeting, infection-site accumulation, and controlled release. Such platforms can additionally enhance biofilm penetration, enable intracellular delivery, and permit co-delivery with conventional antibiotics to achieve synergy. Integrating repurposed agents with advanced DDS could therefore address key resistance mechanisms, including biofilm formation, efflux activity, and intracellular persistence, although substantial challenges remain despite encouraging preclinical data. This review critically evaluates the antimicrobial potential of repurposed non-antimicrobial drugs spanning anti-arthritic, anthelmintic, antineoplastic, psychiatric, and cardiovascular classes, examining mechanisms of action, efficacy evidence, and principal limitations, and assesses how advanced DDS might improve their therapeutic performance against MDR infections. It further identifies major evidence gaps and research priorities to guide development of clinically viable anti-AMR strategies. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of Applied Microbiology International. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Abdulkarim, Meer M AU - Abdulkarim MM AD - Department of Scientific Affairs, Smart Health Tower, Madam Mitterrand Street, Sulaymaniyah, Iraq. FAU - Fattah, Fattah H AU - Fattah FH AD - College of Medicine, University of Sulaimani, Madam Mitterrand Street, Sulaymaniyah, Iraq. FAU - Hamasaeed, Ahmed G AU - Hamasaeed AG AD - Department of Pharmacy, Smart Health Tower, Madam Mitterrand Street, Sulaymaniyah, Iraq. AD - Faculty of Medical Sciences, School of Pharmacy, University of Sulaimani, Madam Mitterrand Street, Sulaymaniyah, Iraq. FAU - Anwar, Khanda A AU - Anwar KA AD - College of Medicine, University of Sulaimani, Madam Mitterrand Street, Sulaymaniyah, Iraq. FAU - Ezzat, Shvan L AU - Ezzat SL AD - Department of Laboratory, Smart Health Tower, Madam Mitterrand Street, Sulaymaniyah, Iraq. FAU - Mustafa, Ayman M AU - Mustafa AM AD - Department of Scientific Affairs, Smart Health Tower, Madam Mitterrand Street, Sulaymaniyah, Iraq. AD - Kscien Organization for Scientific Research (Middle East Office), Hamdi Street, Sulaymaniyah, Iraq. FAU - Mohammed, Karokh K AU - Mohammed KK AD - Kscien Organization for Scientific Research (Middle East Office), Hamdi Street, Sulaymaniyah, Iraq. FAU - Mohammed, Shvan H AU - Mohammed SH AD - Kscien Organization for Scientific Research (Middle East Office), Hamdi Street, Sulaymaniyah, Iraq. FAU - Mahmood, Zhiran F AU - Mahmood ZF AD - Department of Pharmacy, Smart Health Tower, Madam Mitterrand Street, Sulaymaniyah, Iraq. FAU - Mohammed, Rebar A AU - Mohammed RA AD - Department of Pharmacy, Smart Health Tower, Madam Mitterrand Street, Sulaymaniyah, Iraq. FAU - Hama, Piroz Rahim AU - Hama PR AD - Department of Pharmacy, Smart Health Tower, Madam Mitterrand Street, Sulaymaniyah, Iraq. FAU - Kakamad, Fahmi H AU - Kakamad FH AD - Kscien Organization for Scientific Research (Middle East Office), Hamdi Street, Sulaymaniyah, Iraq. AD - Department of Thoracic and Vascular Surgery, Smart Health Tower, Madam Mitterrand Street, Sulaymaniyah, Iraq. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - J Appl Microbiol JT - Journal of applied microbiology JID - 9706280 SB - IM OTO - NOTNLM OT - ESKAPE pathogens OT - Multi-drug resistance OT - advanced drug delivery systems OT - drug repurposing EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 08:14 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:14 [entrez] AID - 9004465 [pii] AID - 10.1093/jambio/lxag268 [doi] PST - aheadofprint SO - J Appl Microbiol. 2026 Oct 29:lxag268. doi: 10.1093/jambio/lxag268. PMID- 42954394 OWN - NLM STAT- Publisher LR - 20261029 IS - 1574-6968 (Electronic) IS - 0378-1097 (Linking) DP - 2026 Oct 29 TI - Paper Strip-Based Sensor for Detection of Antimicrobial Resistant Listeria monocytogenes in Milk. LID - fnag132 [pii] LID - 10.1093/femsle/fnag132 [doi] AB - To develop a rapid, selective, and field-deployable paper strip assay for the detection of Listeria monocytogenes and targeted screening of resistance to Penicillin G and Ampicillin in milk. A novel Listeria Selective Enrichment Medium (LSEM) was optimized to enhance the recovery of L. monocytogenes in milk while reducing interference from selected competing microorganisms evaluated in this study. The assay employed beta-D-glucosidase and enzyme-substrate reactions for presumptive and species-specific detection, producing characteristic colour changes for Listeria spp. The developed paper strip detected L. monocytogenes within 7 h in broth and 9 h (with enrichment for 24 h at 1 CFU/25 mL) in spiked sterilized milk. The total detection time of the developed assay was approximately 33 h (24 h enrichment + 9 h strip-based confirmation). In the present study, antimicrobial resistance assessment was limited to two representative beta-lactam antibiotics, Penicillin G and Ampicillin. Therefore, the assay should currently be considered a targeted screening platform rather than a universal antimicrobial susceptibility test. Resistant strains showed colour development in the presence of the respective antibiotic, whereas susceptible strains remained colourless. Validation with 70 milk samples confirmed one raw milk sample positive for L. monocytogenes, which was further verified using ISO 11290-1:2017 and disc diffusion methods. The developed assay provides a rapid, selective, cost-effective, and user-friendly platform for simultaneous detection of L. monocytogenes and targeted susceptibility screening to Penicillin G and Ampicillin in milk. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of FEMS. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Talan, Shreya AU - Talan S AD - Microbial Biosensor, Food Safety and Quality Assurance Laboratory, Dairy Microbiology Division, ICAR - National Dairy Research Institute, Karnal, Haryana 132001, India. FAU - Balhara, Mandeep AU - Balhara M AD - Microbial Biosensor, Food Safety and Quality Assurance Laboratory, Dairy Microbiology Division, ICAR - National Dairy Research Institute, Karnal, Haryana 132001, India. FAU - Kouser, Shabnam AU - Kouser S AD - Microbial Biosensor, Food Safety and Quality Assurance Laboratory, Dairy Microbiology Division, ICAR - National Dairy Research Institute, Karnal, Haryana 132001, India. FAU - Kumar, Naresh AU - Kumar N AD - Microbial Biosensor, Food Safety and Quality Assurance Laboratory, Dairy Microbiology Division, ICAR - National Dairy Research Institute, Karnal, Haryana 132001, India. FAU - Vishweswaraiah, Raghu Hirikyathanahalli AU - Vishweswaraiah RH AUID- ORCID: 0000-0002-4166-0086 AD - Microbial Biosensor, Food Safety and Quality Assurance Laboratory, Dairy Microbiology Division, ICAR - National Dairy Research Institute, Karnal, Haryana 132001, India. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - FEMS Microbiol Lett JT - FEMS microbiology letters JID - 7705721 SB - IM OTO - NOTNLM OT - Listeria monocytogenes OT - antimicrobial resistance OT - milk safety OT - paper strip biosensor OT - rapid detection OT - selective enrichment medium EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 08:14 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:14 [entrez] AID - 9004470 [pii] AID - 10.1093/femsle/fnag132 [doi] PST - aheadofprint SO - FEMS Microbiol Lett. 2026 Oct 29:fnag132. doi: 10.1093/femsle/fnag132. PMID- 42954392 OWN - NLM STAT- Publisher LR - 20261029 IS - 1574-6968 (Electronic) IS - 0378-1097 (Linking) DP - 2026 Oct 29 TI - Microbiome science needs AI competence, not just AI literacy. LID - fnag133 [pii] LID - 10.1093/femsle/fnag133 [doi] AB - Artificial intelligence is rapidly becoming embedded in microbiome research, yet the way microbiome scientists are trained has not kept pace. Existing discussions frequently emphasize AI literacy and responsible tool use. We argue that literacy alone is insufficient in a field where data leakage, incomplete metadata, ecological confounding, inappropriate model selection and unverified generative-AI outputs can produce convincing but biologically misleading conclusions. We propose an AI-competence framework comprising five connected capabilities: data and platform fluency, machine-learning competence, advanced model competence, large-language-model and agent competence, and biological closure. The final capability-the ability to connect computational predictions to independent data, mechanistic reasoning or experimental evidence-is essential for ensuring that predictive performance becomes meaningful biology. We further propose that competence should be demonstrated through reproducible scientific artifacts rather than course completion alone. Shared datasets, computational platforms and benchmark challenges could make such training broadly accessible while establishing common standards for evaluating AI competence in microbiome science. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of FEMS. FAU - Anand, Archana AU - Anand A AUID- ORCID: 0000-0002-1834-6187 AD - Department of Biology, San Francisco State University, San Francisco, CA 94132, USA. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - FEMS Microbiol Lett JT - FEMS microbiology letters JID - 7705721 SB - IM OTO - NOTNLM OT - artificial intelligence OT - bioinformatics education OT - machine learning OT - microbiome science OT - reproducibility OT - workforce development EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 08:14 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:14 [entrez] AID - 9004453 [pii] AID - 10.1093/femsle/fnag133 [doi] PST - aheadofprint SO - FEMS Microbiol Lett. 2026 Oct 29:fnag133. doi: 10.1093/femsle/fnag133. PMID- 42954383 OWN - NLM STAT- Publisher LR - 20261029 IS - 1574-6941 (Electronic) IS - 0168-6496 (Linking) DP - 2026 Oct 29 TI - Microbial profiling of Drosophila in French Guiana reveals candidate microbial taxa associated with native and invasive host. LID - fiag121 [pii] LID - 10.1093/femsec/fiag121 [doi] AB - Invasive alien species (IAS) threaten biodiversity, human health, and economies, yet host-associated microbiome differences between invasive and native species remain poorly understood in natural systems. We investigated bacterial and fungal microbiomes of IAS and native Drosophila species collected along an anthropisation gradient in French Guiana. IAS occurrence declined strongly from coastal anthropised habitats toward rainforest localities. Using bacterial 16S rRNA and fungal ITS metabarcoding of pooled flies, we assessed microbial diversity and community structure across host species, host category (invasive vs. native), and locality. IAS-associated bacterial communities showed reduced alpha diversity and differed in structure from those of native Drosophila. Bacteriome variation was also associated with host phylogeny and locality; because host category was partially confounded with these factors, differences cannot be attributed solely to invasion status. Fungal communities were primarily associated with locality and host species. We further identified core and exclusive taxa and compared bacteriome composition with global reference datasets and microbiome literature, identifying bacterial and fungal taxa preferentially or exclusively associated with invasive or native hosts. Overall, our study characterises microbiome variation between invasive and native Drosophila in French Guiana and identifies candidate taxa for future functional investigation. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of FEMS. FAU - Laffargue, Thibault T AU - Laffargue TT AUID- ORCID: 0009-0005-0641-767X AD - Laboratoire Ecologie, Evolution, Interactions des systemes Amazoniens, University of French Guiana, CNRS, IFREMER, Cayenne, France. AD - EGCE, University Paris-Saclay, CNRS, IRD, Gif-sur-Yvette, France. FAU - Pollet, Nicolas AU - Pollet N AUID- ORCID: 0000-0002-9975-9644 AD - EGCE, University Paris-Saclay, CNRS, IRD, Gif-sur-Yvette, France. FAU - Brousseau, Louise AU - Brousseau L AD - UMR AMAP, University of Montpellier, IRD, CIRAD, CNRS, INRAE, Montpellier, France. FAU - Miller, Wolfgang J AU - Miller WJ AUID- ORCID: 0000-0002-5044-6276 AD - Center of Anatomy and Cell Biology, Medical University of Vienna, Austria. FAU - Hua-Van, Aurelie AU - Hua-Van A AUID- ORCID: 0000-0001-7859-2788 AD - EGCE, University Paris-Saclay, CNRS, IRD, Gif-sur-Yvette, France. FAU - Chouteau, Mathieu AU - Chouteau M AD - Laboratoire Ecologie, Evolution, Interactions des systemes Amazoniens, University of French Guiana, CNRS, IFREMER, Cayenne, France. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - FEMS Microbiol Ecol JT - FEMS microbiology ecology JID - 8901229 SB - IM OTO - NOTNLM OT - Drosophila OT - French Guiana OT - Neotropical OT - host adaptability OT - invasive alien species OT - microbiome EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 08:13 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:13 [entrez] AID - 9004468 [pii] AID - 10.1093/femsec/fiag121 [doi] PST - aheadofprint SO - FEMS Microbiol Ecol. 2026 Oct 29:fiag121. doi: 10.1093/femsec/fiag121. PMID- 42954373 OWN - NLM STAT- Publisher LR - 20261029 IS - 1478-6427 (Electronic) IS - 1478-6419 (Linking) DP - 2026 Oct 29 TI - Phytochemical characterization and evaluation of anti-melanogenic of ethanol extract from Trichosanthes costata Blume: in vitro and In silico analysis. PG - 1-14 LID - 10.1080/14786419.2026.2752548 [doi] AB - This study investigated the phytochemical composition and biological effects of Trichosanthes costata extract using both in vitro and in silico approaches. The extract exhibited high flavonoid content, strong antioxidant capacity, and selective cytotoxicity against B16 melanoma cells. Furthermore, it significantly reduced melanin -synthesis, inhibited tyrosinase activity, and downregulated key melanogenesis-related genes, including tyr, trp1, trp2, and mitf. A total of 74 phytocompounds were identified using GC-MS and LC-MS analyses. GC-MS analysis revealed major constituents, including 2-pentanone (43.01%), taraxasterol (27.14%), and lupeol (9.72%), while LC-MS analysis identified N-isobutyl-(2E,4E)-octadecadienamide (82.103%) as a predominant compound. Among the identified phytochemicals, taraxasterol exhibited strong binding affinity towards melanogenesis-related proteins, suggesting its potential role in melanin regulation. Collectively, this study provides the first evidence of anti-melanogenic activity in T. costata, revealing previously uncharacterised bioactivities and supporting the potential repurposing of taraxasterol as potential natural candidates for the treatment of hyperpigmentation disorders. FAU - Huynh, Chau Dao Minh AU - Huynh CDM AD - Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam. AD - Vietnam National University, Ho Chi Minh City, Vietnam. FAU - Nguyen-Van, Minh-Thuong AU - Nguyen-Van MT AD - Vietnam National University, Ho Chi Minh City, Vietnam. AD - Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam. AD - Laboratory of Molecular Biotechnology, University of Science, Ho Chi Minh City, Vietnam. FAU - Le, Thang Truong AU - Le TT AD - School of Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan. AD - Genome and Systems Biology Degree Program, National Taiwan University and Academia Sinica, Taipei, Taiwan. FAU - Nguyen, Tien-Dung AU - Nguyen TD AD - Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam. AD - Vietnam National University, Ho Chi Minh City, Vietnam. FAU - Bui, Chau Dieu AU - Bui CD AD - Vietnam National University, Ho Chi Minh City, Vietnam. AD - Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam. AD - Laboratory of Molecular Biotechnology, University of Science, Ho Chi Minh City, Vietnam. FAU - Dao, Phuc Nguyen Thien AU - Dao PNT AD - Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam. AD - Vietnam National University, Ho Chi Minh City, Vietnam. FAU - Hoang, Viet AU - Hoang V AD - Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam. AD - Vietnam National University, Ho Chi Minh City, Vietnam. FAU - Tran-Van, Hieu AU - Tran-Van H AUID- ORCID: 0000-0003-2782-5232 AD - Vietnam National University, Ho Chi Minh City, Vietnam. AD - Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam. AD - Laboratory of Biosensors, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam. AD - Laboratory of Molecular Biotechnology, University of Science, Ho Chi Minh City, Vietnam. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Nat Prod Res JT - Natural product research JID - 101167924 SB - IM OTO - NOTNLM OT - Trichosanthes costata OT - antioxidant activity OT - melanogenesis inhibition OT - phytochemical profiling OT - tyrosinase activity EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 08:13 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:13 [entrez] AID - 10.1080/14786419.2026.2752548 [doi] PST - aheadofprint SO - Nat Prod Res. 2026 Oct 29:1-14. doi: 10.1080/14786419.2026.2752548. PMID- 42954364 OWN - NLM STAT- Publisher LR - 20261029 IS - 1535-4989 (Electronic) IS - 1044-1549 (Linking) DP - 2026 Oct 29 TI - 17beta-Estradiol and Estrogen Receptor alpha Promote Right Ventricle Angiogenesis in Pulmonary Hypertension via Apelin Signaling. LID - aanag177 [pii] LID - 10.1093/ajrcmb/aanag177 [doi] AB - Right ventricular (RV) failure is the major cause of mortality in pulmonary hypertension (PH). Adaptive angiogenesis and RV endothelial cell (RVEC) function are major modifiers of RV adaptation in PH, but the underlying mechanisms and their regulators remain incompletely understood. RV adaptation in PH is sexually dimorphic, and 17beta-estradiol (E2) exerts protective effects on RV cardiomyocytes. Whether E2 modifies angiogenesis and RVEC function in RV failure remains unknown. We hypothesized that E2 and estrogen receptor alpha (ERalpha) promote RV angiogenesis and RVEC homeostasis in PH and aimed to identify underlying mechanisms. We assessed E2's angiogenic effects using cultured human cardiac microvascular endothelial cells (hCMVECs), RVECs from PH patients with RV failure, and RVECs from sugen/hypoxia (SuHx) and monocrotaline rat models. In vivo, we evaluated RV capillary density in PH rats treated with E2 or ERalpha-selective agonist. Apelin signaling was evaluated via apelin receptor (APLNR) blockade and knockdown studies. E2 preserved angiogenesis in male hCMVECs and RV capillary density in male and female SuHx-PH rats. E2 reversed angiogenic alterations in RVECs from SuHx-PH rats via APLNR signaling. In RVECs from RV failure patients, E2 stimulated vascular network formation. In rat and human PH-RVECs, ERalpha was necessary and sufficient to mediate E2-induced angiogenesis. Activation of ERalpha with ERalpha-specific agonist restored RV capillary density in vivo. ERalpha-mediated angiogenesis required apelin signaling. These data indicate that E2 promotes RV angiogenesis via ERalpha and apelin signaling and identify a novel ERalpha-apelin axis in RVECs as a potential therapeutic target to restore RV vascular integrity in PH. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the American Thoracic Society. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Bousseau, Simon AU - Bousseau S AD - National Jewish Health, Denver, CO, USA. FAU - Frump, Andrea L AU - Frump AL AD - Indiana University School of Medicine, Indianapolis, IN, USA. FAU - Walts, Avram AU - Walts A AD - National Jewish Health, Denver, CO, USA. FAU - Sobrano Fais, Rafael AU - Sobrano Fais R AD - National Jewish Health, Denver, CO, USA. FAU - Fisher, Amanda AU - Fisher A AD - Indiana University School of Medicine, Indianapolis, IN, USA. FAU - Bharti, Manisha AU - Bharti M AD - Indiana University School of Medicine, Indianapolis, IN, USA. FAU - Yakubov, Bakhtiyor AU - Yakubov B AD - Indiana University School of Medicine, Indianapolis, IN, USA. FAU - Provencher, Steeve AU - Provencher S AD - Laval University, Quebec City, QC, Canada. FAU - Breuils Bonnet, Sandra AU - Breuils Bonnet S AD - Laval University, Quebec City, QC, Canada. FAU - Bonnet, Sebastien AU - Bonnet S AD - Laval University, Quebec City, QC, Canada. FAU - Lahm, Tim AU - Lahm T AD - National Jewish Health, Denver, CO, USA. AD - University of Colorado, Anschutz Medical Campus, Aurora, CO, USA. AD - Rocky Mountain Regional VA Medical Center, Aurora, CO, USA. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Am J Respir Cell Mol Biol JT - American journal of respiratory cell and molecular biology JID - 8917225 SB - IM OTO - NOTNLM OT - Endothelial cell function OT - angiogenesis OT - right heart failure OT - sex hormones EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 08:03 PHST- 2025/12/09 00:00 [received] PHST- 2026/07/21 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:03 [entrez] AID - 9004409 [pii] AID - 10.1093/ajrcmb/aanag177 [doi] PST - aheadofprint SO - Am J Respir Cell Mol Biol. 2026 Oct 29:aanag177. doi: 10.1093/ajrcmb/aanag177. PMID- 42954362 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2045-7634 (Electronic) IS - 2045-7634 (Linking) VI - 15 IP - 11 DP - 2026 Nov TI - Neoadjuvant Therapy for Non-Small Cell Lung Cancer: Recent Advances and Future Directions. LID - e72350 [pii] LID - 10.1002/cam4.72350 [doi] AB - Neoadjuvant therapy has become an integral component of the multidisciplinary management of resectable non-small cell lung cancer (NSCLC). The introduction of immune checkpoint inhibitors (ICIs) has transformed the perioperative treatment landscape by improving pathological response rates, reducing the risk of recurrence, and offering the potential for durable survival benefits. In recent years, multiple neoadjuvant strategies, including ICI monotherapy, chemoimmunotherapy, dual immune checkpoint blockade, and combinations with radiotherapy, anti-angiogenic agents, or targeted therapies, have been actively investigated to further optimize treatment efficacy. Immune checkpoint inhibitors (ICIs) are reshaping neoadjuvant strategies for resectable NSCLC. LCMC3 confirmed the feasibility of ICIs monotherapy, while CheckMate 816 and NADIM demonstrated that ICI-chemotherapy combinations markedly improve major pathological response and survival outcomes. Emerging perioperative studies, including CheckMate 77 T and AEGEAN, further support the integration of immunotherapy into standard treatment paradigms. Radiotherapy enhances tumor immunogenicity and shows synergy with ICIs, as highlighted in PACIFIC and KEYNOTE-799. Although clinical evidence for ICI-anti-angiogenic combinations remains preliminary, their mechanistic rationale is strong. In EGFR- or ALK-altered NSCLC, emerging data suggest that neoadjuvant targeted therapy and carefully selected immunotherapy-based strategies may further improve disease control while delaying recurrence. Despite these advances, several challenges remain, including the paucity of long-term survival data, discordance between pathological responses and clinical benefit, treatment-related toxicities, optimal treatment sequencing, biomarker-guided patient selection, and mechanisms of primary or acquired resistance. Advances in predictive modeling based on PD-L1 expression, tumor mutational burden (TMB), radiomics, nomograms, circulating tumor DNA, and multi-omics biomarkers, together with a deeper understanding of metabolic-immune crosstalk, are expected to facilitate precision perioperative immunotherapy. This review comprehensively summarizes current evidence regarding neoadjuvant immunotherapy strategies for resectable NSCLC, highlights pivotal clinical trial findings, discusses emerging combination approaches and predictive biomarkers, and outlines future research directions aimed at improving personalized treatment and long-term patient outcomes. CI - (c) 2026 The Author(s). Cancer Medicine published by John Wiley & Sons Ltd. FAU - An, Hao AU - An H AD - School of Clinical Medicine, Shandong Second Medical University, Weifang, China, Shandong Cancer Hospital and Institute, Affiliated Hospital of Weifang Medical University, School of Clinical Medicine, Weifang Medical University, Weifang, Shandong, China. FAU - Zhao, Junfeng AU - Zhao J AD - Department of Radiation Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, China. FAU - Chen, Wenjie AU - Chen W AD - Department of Respiratory Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, China. FAU - Li, Ying AU - Li Y AD - Department of Respiratory Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, China. FAU - Yan, Yifei AU - Yan Y AD - Shandong First Medical University, Jinan, Shandong, China. FAU - Zhou, Chen AU - Zhou C AD - Shandong First Medical University, Jinan, Shandong, China. FAU - Li, Ruyue AU - Li R AD - School of Clinical Medicine, Shandong Second Medical University, Weifang, China, Shandong Cancer Hospital and Institute, Affiliated Hospital of Weifang Medical University, School of Clinical Medicine, Weifang Medical University, Weifang, Shandong, China. FAU - Yao, Xiujing AU - Yao X AD - School of Clinical Medicine, Shandong Second Medical University, Weifang, China, Shandong Cancer Hospital and Institute, Affiliated Hospital of Weifang Medical University, School of Clinical Medicine, Weifang Medical University, Weifang, Shandong, China. FAU - Dong, Xue AU - Dong X AD - Department of Respiratory Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, China. FAU - Yang, Xingying AU - Yang X AD - Department of Respiratory Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, China. FAU - Hu, Yongjuan AU - Hu Y AD - Department of Respiratory Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, China. FAU - Chen, Jiaxuan AU - Chen J AD - Shandong First Medical University, Jinan, Shandong, China. FAU - Han, Chuankun AU - Han C AD - Shandong First Medical University, Jinan, Shandong, China. FAU - Bai, Yingrui AU - Bai Y AD - Weifang Medical University, Weifang, Shandong, China. FAU - Xu, Yali AU - Xu Y AUID- ORCID: 0000-0002-4945-6135 AD - Department of Pathology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. FAU - Li, Yintao AU - Li Y AD - Department of Respiratory Oncology, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, Shandong, China. LA - eng GR - 82373044/National Natural Science Foundation of China/ GR - ZR2023LSW023/Natural Science Foundation of Shandong Province/ GR - 2023ZD0501900/Non-communicable Chronic Diseases-National Science and Technology Major Project/ GR - 2023ZD0501904/Non-communicable Chronic Diseases-National Science and Technology Major Project/ GR - 2023ZD0501905/Non-communicable Chronic Diseases-National Science and Technology Major Project/ PT - Journal Article PT - Review PL - United States TA - Cancer Med JT - Cancer medicine JID - 101595310 RN - 0 (Immune Checkpoint Inhibitors) SB - IM MH - Humans MH - *Neoadjuvant Therapy/methods MH - *Carcinoma, Non-Small-Cell Lung/therapy/pathology MH - *Lung Neoplasms/therapy/pathology MH - *Immune Checkpoint Inhibitors/therapeutic use MH - Immunotherapy/methods OTO - NOTNLM OT - clinical trials OT - combination therapy OT - immune checkpoint inhibitors OT - neoadjuvant immunotherapy OT - non-small cell lung cancer OT - pathological response tumor EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 08:03 PHST- 2026/06/26 00:00 [revised] PHST- 2026/01/13 00:00 [received] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 08:03 [entrez] AID - e72350 [pii] AID - 10.1002/cam4.72350 [doi] PST - ppublish SO - Cancer Med. 2026 Nov;15(11):e72350. doi: 10.1002/cam4.72350. PMID- 42954352 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2050-084X (Electronic) IS - 2050-084X (Linking) VI - 15 DP - 2026 Oct 29 TI - Revealing the benefit of eye motion for acuity under emulated cone loss. LID - 10.7554/eLife.111289 [doi] LID - RP111289 AB - Retinal degenerative diseases progressively erode the cone photoreceptor mosaic, reducing the retina's spatial sampling power, yet visual acuity is remarkably resilient to cone loss. Prior work has shown that clinically normal visual acuity (20/25 or better) can persist despite up to 50% of cone cells being lost (Ratnam et al., 2013; Foote et al., 2018). However, studies on individuals with retinal degeneration cannot control for patients' stage of disease progression, creating the need for an experimental paradigm that can mimic these diseases in healthy subjects. The Oz Vision system creates visual percepts through programmable, per-cell stimulation of thousands of cone cells. We reprogram this system to emulate cone loss in healthy eyes by withholding stimulation from a subset of randomly selected cones, rendering them inactive, in a method we term 'cone dropout'. Using this approach, we characterize the visual system's robustness to cone loss, showing that visual acuity declines nonlinearly with increasing cone dropout. Importantly, we uncover the compensatory benefit that eye motion provides under cone-deprived conditions, finding that at the highest level of dropout, a visual system with eye motion has an equivalent acuity to a static dropout condition with nearly twice as many sampling elements. Through analysis of eye motion and stimulation data, we find that this benefit arises from the additional information accumulated by 'surviving' cones as they sample more of the letter through fixational eye motion. CI - (c) 2026, Doyle et al. FAU - Doyle, Hannah K AU - Doyle HK AUID- ORCID: 0009-0003-3101-8588 AD - Department of Electrical Engineering & Computer Sciences, University of California, Berkeley, Berkeley, United States. FAU - Fong, James AU - Fong J AUID- ORCID: 0009-0009-5106-8404 AD - Department of Electrical Engineering & Computer Sciences, University of California, Berkeley, Berkeley, United States. FAU - Ng, Ren AU - Ng R AUID- ORCID: 0009-0007-2207-021X AD - Department of Electrical Engineering & Computer Sciences, University of California, Berkeley, Berkeley, United States. FAU - Roorda, Austin AU - Roorda A AUID- ORCID: 0000-0002-3785-0848 AD - Herbert Wertheim School of Optometry & Vision Science, University of California, Berkeley, Berkeley, United States. LA - eng GR - FA9550-21-1-0230/Air Force Office of Scientific Research/ GR - R01EY023591/NH/NIH HHS/United States PT - Journal Article DEP - 20261029 PL - England TA - Elife JT - eLife JID - 101579614 SB - IM UOF - doi: 10.64898/2026.03.19.712913 UOF - doi: 10.7554/eLife.111289.1 UOF - doi: 10.7554/eLife.111289.2 MH - *Retinal Cone Photoreceptor Cells/physiology/pathology MH - Humans MH - *Visual Acuity/physiology MH - *Eye Movements/physiology MH - *Retinal Degeneration/physiopathology PMC - PMC13719164 OTO - NOTNLM OT - adaptive optics OT - eye movements OT - human OT - neuroscience OT - photoreceptors OT - scanning laser ophthalmoscopy OT - visual acuity COIS- HD, JF No competing interests declared, RN, AR The Regents of the University of California has filed a patent for cell-by-cell retina stimulation, for which R.N. and A.R. are inventors (WO2020086612A1) EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/29 CRDT- 2026/10/29 07:52 PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 07:52 [entrez] PHST- 2026/10/29 00:00 [pmc-release] AID - 111289 [pii] AID - 10.7554/eLife.111289 [doi] PST - epublish SO - Elife. 2026 Oct 29;15:RP111289. doi: 10.7554/eLife.111289. PMID- 42954346 OWN - NLM STAT- Publisher LR - 20261029 IS - 1525-1594 (Electronic) IS - 0160-564X (Linking) DP - 2026 Oct 29 TI - Utilization of Microaxial Mechanical Circulatory Support Among the Pediatric Population: A Systematic Review. LID - 10.1111/aor.70260 [doi] AB - BACKGROUND: Utilization of microaxial pumps for temporary mechanical circulatory support in children has increased in recent years. However, data on patient selection, implantation techniques, and outcomes remain limited. METHODS: A systematic literature search was conducted in July 2025 to identify case reports and case series reporting the use of microaxial pumps in patients aged 18 years or less. Forty-four studies comprising 100 patients were identified, and patient-level data were extracted for analysis. RESULTS: The median age of the patients was 14 [IQR, 11.5-16] years, with a median weight of 52 [40-73] kg and median body surface area of 1.59 [1.35-1.85] m(2). The most common diagnoses were cardiomyopathy (29%), heart allograft rejection (26%), and myocarditis (21%). Impella CP was the most frequently used device (48%), followed by Impella 2.5 and Impella 5.5 (each 21%). Access was predominantly obtained via the femoral artery (47%). Percutaneous implantation was performed in 47% of patients. Patients were weaned from microaxial support for cardiac recovery (42%) or heart transplantation (31%). Fourteen patients (14%) died prior to discharge, with significantly higher mortality among patients supported with ECPella (29%) compared with microaxial pump alone (6%; p = 0.01). The median duration of support was 6 [4-13] days. At the median follow-up of 119 [23-180] days overall survival was 84%. CONCLUSION: Microaxial pumps represent an important addition to temporary mechanical circulatory support in pediatric patients and can serve as an alternative to VA-ECMO in selected cases. Outcomes were less favorable when used in tandem configurations such as ECPella, reflecting the greater severity of illness in these patients. CI - (c) 2026 International Center for Artificial Organ and Transplantation (ICAOT) and Wiley Periodicals LLC. FAU - Jones, Aryana J AU - Jones AJ AUID- ORCID: 0009-0005-7816-6865 AD - Department of Cardiac Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania, USA. FAU - Rahimov, Daler AU - Rahimov D AUID- ORCID: 0009-0004-8055-6101 AD - Department of Cardiac Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania, USA. FAU - Smoot, Dylan AU - Smoot D AUID- ORCID: 0009-0004-6618-003X AD - Drexel University College of Medicine, Philadelphia, Pennsylvania, USA. FAU - Mustafa, Manal AU - Mustafa M AUID- ORCID: 0009-0008-1901-373X AD - Department of Cardiac Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania, USA. FAU - Mallur, Keerti AU - Mallur K AD - Department of Cardiac Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania, USA. FAU - Rajab, Taufiek Konrad AU - Rajab TK AUID- ORCID: 0000-0001-8885-2758 AD - Department of Cardiovascular Pediatric Surgery, Arkansas Children's Hospital, Little Rock, Arkansas, USA. FAU - Hoopes, Charles W AU - Hoopes CW AD - Department of Cardiac Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania, USA. FAU - Tchantchaleishvili, Vakhtang AU - Tchantchaleishvili V AD - Department of Cardiac Surgery, Thomas Jefferson University, Philadelphia, Pennsylvania, USA. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - United States TA - Artif Organs JT - Artificial organs JID - 7802778 SB - IM OTO - NOTNLM OT - Impella OT - mechanical circulatory support OT - microaxial pump OT - pediatric patients EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 07:42 PHST- 2026/08/29 00:00 [revised] PHST- 2026/02/02 00:00 [received] PHST- 2026/09/04 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 07:42 [entrez] AID - 10.1111/aor.70260 [doi] PST - aheadofprint SO - Artif Organs. 2026 Oct 29. doi: 10.1111/aor.70260. PMID- 42954345 OWN - NLM STAT- Publisher LR - 20261029 IS - 1521-4095 (Electronic) IS - 0935-9648 (Linking) DP - 2026 Oct 29 TI - A Magnetic Robotic Platform for Enhanced Stem-Cell-Based Tendon Repair. LID - e75562 [pii] LID - 10.1002/adma.75562 [doi] AB - Robotics enables regenerative medicine to be more precise, less invasive, and more targeted. Mesenchymal stem cell (MSC) therapy shows great potential for tissue regeneration, but challenges such as low cell survival and limited differentiation ability after in vivo implantation hinder its clinical translation. In this study, we propose a magnetic robotic platform capable of autonomously recognizing and targeting injury sites, as well as delivering noninvasive magnetic stimuli to programmatically regulate MSC functions for tendon repair. This platform integrates 3D-MRI with real-time recognition to enable spatial perception of deep tissues, which is visualized through panoramic simulation. It provides an engineering-precise and intelligent automated magnetic stimulation for the diverse tendon injuries scenarios. We identified pulse electromagnetic field (PEMF) parameters that induced proliferation and tenogenesis of MSCs, respectively, and demonstrated the feasibility of programmed PEMF to sequentially regulate cell behaviors of transplanted MSCs, which contributed to the structure and functional recovery of rat injured tendons. Furthermore, we elucidated the underlying molecular mechanisms by which different magnetic stimuli regulate MSC functions, providing insight into PEMF-mediated MSCs responses. Generally, this magnetic robotic system offers a noninvasive, precise, and biosafe strategy to enhance MSC-based therapies, with significant implications for clinical applications and broader regenerative medicine. CI - (c) 2026 Wiley-VCH GmbH. FAU - Cao, Mumin AU - Cao M AUID- ORCID: 0000-0003-2227-198X AD - Department of Orthopaedics, Orthopaedic Trauma Institute (OTI), Trauma Center, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu, P. R. China. FAU - Sheng, Renwang AU - Sheng R AD - Department of Orthopaedics, Orthopaedic Trauma Institute (OTI), Trauma Center, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu, P. R. China. FAU - Sun, Yimin AU - Sun Y AUID- ORCID: 0009-0009-6253-305X AD - Jiangsu Key Laboratory for Design and Manufacturing of Precision Medicine Equipment, School of Mechanical Engineering, Southeast University, Nanjing, Jiangsu, P. R. China. AD - Department of Robotics, School of Mechanical Engineering, Southeast University, Nanjing, Jiangsu, P. R. China. FAU - Cao, Ying AU - Cao Y AUID- ORCID: 0009-0003-2651-5854 AD - Jiangsu Key Laboratory for Design and Manufacturing of Precision Medicine Equipment, School of Mechanical Engineering, Southeast University, Nanjing, Jiangsu, P. R. China. AD - Department of Robotics, School of Mechanical Engineering, Southeast University, Nanjing, Jiangsu, P. R. China. FAU - Lv, Xuepeng AU - Lv X AD - State Key Laboratory of Digital Medical Engineering, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Sciences & Medical Engineering, Southeast University, Nanjing, P. R. China. FAU - Zhang, Hang AU - Zhang H AUID- ORCID: 0000-0002-5187-8732 AD - Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, P. R. China. FAU - Wang, Hao AU - Wang H AD - Department of Orthopaedics, Orthopaedic Trauma Institute (OTI), Trauma Center, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu, P. R. China. FAU - Gao, Yucheng AU - Gao Y AUID- ORCID: 0000-0002-0337-9285 AD - Department of Orthopaedics, Orthopaedic Trauma Institute (OTI), Trauma Center, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu, P. R. China. FAU - Ou, Zhengkuan AU - Ou Z AD - Department of Orthopaedics, Orthopaedic Trauma Institute (OTI), Trauma Center, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu, P. R. China. FAU - Zhu, Hongkang AU - Zhu H AUID- ORCID: 0000-0003-4734-556X AD - School of Food Science and Engineering, Yangzhou University, Yangzhou, P. R. China. FAU - Xiong, Fei AU - Xiong F AUID- ORCID: 0000-0002-3680-4292 AD - State Key Laboratory of Digital Medical Engineering, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Sciences & Medical Engineering, Southeast University, Nanjing, P. R. China. FAU - Teng, Gao-Jun AU - Teng GJ AUID- ORCID: 0000-0002-5951-4538 AD - Center of Interventional Radiology and Vascular Surgery, Department of Radiology, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, P. R. China. FAU - Wang, Qianqian AU - Wang Q AUID- ORCID: 0000-0001-8011-171X AD - Jiangsu Key Laboratory for Design and Manufacturing of Precision Medicine Equipment, School of Mechanical Engineering, Southeast University, Nanjing, Jiangsu, P. R. China. AD - Department of Robotics, School of Mechanical Engineering, Southeast University, Nanjing, Jiangsu, P. R. China. FAU - Rui, Yunfeng AU - Rui Y AUID- ORCID: 0000-0001-9019-5531 AD - Department of Orthopaedics, Orthopaedic Trauma Institute (OTI), Trauma Center, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, Jiangsu, P. R. China. LA - eng GR - 52575652/National Natural Science Foundation of China/ GR - BK20221462/Natural Science Foundation of Jiangsu Province/ GR - CXJH_SEU 25231/SEU Innovation Capability Enhancement Plan for Doctoral Students/ GR - MSV202614/State Key Laboratory of Mechanical System and Vibration/ GR - 2024-O04/State Key Laboratory of Robotics and Intelligent Systems/ PT - Journal Article DEP - 20261029 PL - Germany TA - Adv Mater JT - Advanced materials (Deerfield Beach, Fla.) JID - 9885358 SB - IM OTO - NOTNLM OT - field stimulation OT - magnetic control OT - mesenchymal stem cell OT - microrobotic system OT - tendon injury EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 07:42 PHST- 2026/08/07 00:00 [revised] PHST- 2026/03/02 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 07:42 [entrez] AID - e75562 [pii] AID - 10.1002/adma.75562 [doi] PST - aheadofprint SO - Adv Mater. 2026 Oct 29:e75562. doi: 10.1002/adma.75562. PMID- 42954339 OWN - NLM STAT- Publisher LR - 20261029 IS - 1751-8431 (Electronic) IS - 1751-8423 (Linking) DP - 2026 Oct 29 TI - Measuring the functional impact of developmental coordination disorder beyond childhood: a COSMIN systematic review. PG - 1-38 LID - 10.1080/17518423.2026.2750395 [doi] AB - BACKGROUND AND OBJECTIVE: Difficulties in daily performance and participation become increasingly relevant for the identification of Developmental Coordination Disorder (DCD) beyond childhood. Several questionnaires have been developed to assess Criterion B in adolescent and adult populations; however, their measurement properties have not been systematically synthesized. This systematic review aimed to (1) identify instruments designed to assess Criterion B of DCD in adolescents and adults, (2) evaluate their measurement properties following COSMIN methodology, and (3) provide evidence-based recommendations for their use in clinical practice and research. METHODS: A systematic review was conducted according to PRISMA and COSMIN guidelines. Six electronic databases were searched from inception to August 2026. Methodological quality was assessed using the COSMIN Risk of Bias checklist, measurement properties were evaluated against the COSMIN criteria for good measurement properties, and the certainty of evidence was graded using the COSMIN-modified GRADE approach. RESULTS: Nineteen studies evaluating five instruments were included: the Adult Developmental Coordination Disorder/Dyspraxia Checklist (ADC), the Adolescents and Adults Coordination Questionnaire (AAC-Q), the Functional Difficulties Questionnaire-9 (FDQ-9), the Developmental Coordination Disorder Questionnaire (DCDQ), and the Adolescent Motor Competence Questionnaire (AMCQ). Internal consistency and construct validity were the most frequently examined properties, whereas measurement error, and measurement invariance were rarely or never assessed. The ADC was the most extensively investigated instrument; structural validity was insufficient with moderate-quality evidence, whereas sufficient evidence was found for content validity and some aspects of test-retest reliability and construct validity. The AAC-Q showed a promising overall psychometric profile, although evidence for content validity, structural validity and internal consistency remained inconsistent. Evidence for the AMCQ was generally weaker despite its sufficient content validity, while findings for the FDQ-9 and DCDQ were based on only two studies each. CONCLUSION: Evidence supporting instruments designed to assess Criterion B of DCD in adolescents and adults remains limited and heterogeneous. No single instrument currently has sufficiently robust evidence to support firm recommendations across populations and cultural contexts. Further COSMIN-aligned validation studies are needed to strengthen confidence in instrument selection for clinical and research use beyond childhood. FAU - Blanco-Martinez, Nerea AU - Blanco-Martinez N AUID- ORCID: 0009-0002-0657-6708 AD - Departamento de Didacticas Especiais, Universidade de Vigo, Vigo, Espana. AD - Well-Move Research Group, Galicia Sur Health Research Institute (IIS Galicia Sur), SERGAS-UVIGO, Vigo, Espana. FAU - Delgado-Lobete, Laura AU - Delgado-Lobete L AUID- ORCID: 0000-0002-6460-1548 AD - CCSyO: Ciencias del Cuidado, Salud y Ocupacion Research Group, Departamento de Atencion Sociosanitaria, Facultad de Ciencias Sociosanitarias, Universidad de Murcia, Lorca, Espana. AD - INTEGRA SAUDE: Investigacion en Integracion e Promocion da Saude Research Group, Facultad de Ciencias de la Salud, Universidade da Coruna, A Coruna, Espana. FAU - Ayan-Perez, Carlos AU - Ayan-Perez C AUID- ORCID: 0000-0003-3374-843X AD - Departamento de Didacticas Especiais, Universidade de Vigo, Vigo, Espana. AD - Well-Move Research Group, Galicia Sur Health Research Institute (IIS Galicia Sur), SERGAS-UVIGO, Vigo, Espana. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - Dev Neurorehabil JT - Developmental neurorehabilitation JID - 101304394 SB - IM OTO - NOTNLM OT - Adolescents OT - COSMIN OT - adults OT - developmental coordination disorder OT - measurement properties EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 07:33 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 07:33 [entrez] AID - 10.1080/17518423.2026.2750395 [doi] PST - aheadofprint SO - Dev Neurorehabil. 2026 Oct 29:1-38. doi: 10.1080/17518423.2026.2750395. PMID- 42954332 OWN - NLM STAT- Publisher LR - 20261029 IS - 2366-9608 (Electronic) IS - 2366-9608 (Linking) DP - 2026 Oct 29 TI - Precise Sputter Control by Tailored Waveform Biasing for Selective Ablation in Future 2D Electronics Manufacturing. LID - e71162 [pii] LID - 10.1002/smtd.71162 [doi] AB - Precise control of ion energy in plasma processes is pivotal for the selective, damage-free etching that future two-dimensional (2D) electronics demand. Conventional equipment technology with RF sinusoidal waveform biasing is unable to generate the monoenergetic ion energy distribution function (IEDF) required for the selective sputtering of materials that exhibit only marginal differences in threshold ion energy for sputtering. Applying a low-frequency tailored pulse-wave-shaped bias voltage waveform to the substrate electrode of a commercial 200 mm inductively coupled plasma (ICP) plasma reactor results in an IEDF with a full width at half maximum (FWHM) of just 10 eV even at higher ion energies. These narrow IEDFs are used within this study to determine the sputtering thresholds of molybdenum disulfide ( MoS2 ) and selected dielectrics: HfO2 , Ta2O5 , Al2O3 and SiO2 , with the aim of enabling the efficient selective physical etching of dielectric materials relative to 2D materials, and vice versa. Armed with this knowledge, the dielectric environment and layer structure can be designed specifically to the application at hand, ensuring the highest possible selectivity in the patterning process. Additionally, a tailored waveform bias allows MoS2 layers of varying quality to be plasma cleaned below the sputtering threshold energy. CI - (c) 2026 The Author(s). Small Methods published by Wiley-VCH GmbH. FAU - Pletzer, Anton AU - Pletzer A AUID- ORCID: 0009-0003-0414-8643 AD - Microsystems Technology, Ruhr University Bochum, Bochum, Germany. FAU - Giesekus, Jonas AU - Giesekus J AUID- ORCID: 0000-0002-4506-867X AD - Chair of Applied Electrodynamics and Plasma Technology, Ruhr University Bochum, Bochum, Germany. FAU - Litke, Alexander AU - Litke A AUID- ORCID: 0009-0008-6946-6913 AD - Fraunhofer Institute for Microelectronic Circuits and Systems, Duisburg, Germany. FAU - Schulze, Julian AU - Schulze J AUID- ORCID: 0000-0001-7929-5734 AD - Chair of Applied Electrodynamics and Plasma Technology, Ruhr University Bochum, Bochum, Germany. FAU - Bock, Claudia AU - Bock C AUID- ORCID: 0000-0003-0933-9556 AD - Microsystems Technology, Ruhr University Bochum, Bochum, Germany. LA - eng GR - 16ES1096K/Bundesministerium fur Bildung und Forschung/ GR - 16ES0941/Bundesministerium fur Bildung und Forschung/ GR - 590974211/Deutsche Forschungsgemeinschaft/ GR - Federal Ministry of Education and Research of Germany/ PT - Journal Article DEP - 20261029 PL - Germany TA - Small Methods JT - Small methods JID - 101724536 SB - IM OTO - NOTNLM OT - MoS 2 OT - 2D materials OT - IEDF control OT - plasma enhanced atomic layer deposition OT - plasma etching OT - sputter etching OT - voltage waveform tailoring EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 07:33 PHST- 2026/10/07 00:00 [revised] PHST- 2026/08/14 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 07:33 [entrez] AID - e71162 [pii] AID - 10.1002/smtd.71162 [doi] PST - aheadofprint SO - Small Methods. 2026 Oct 29:e71162. doi: 10.1002/smtd.71162. PMID- 42954328 OWN - NLM STAT- Publisher LR - 20261029 IS - 1523-5866 (Electronic) IS - 1522-8517 (Linking) DP - 2026 Oct 29 TI - Standard treatment against paediatric BRAF-V600E glioma promotes senescence and sensitizes tumours to BCL-xL inhibition. LID - noag270 [pii] LID - 10.1093/neuonc/noag270 [doi] AB - BACKGROUND: Chemo-, radio-, and targeted therapies can induce a stable growth arrest known as therapy-induced senescence (TIS), which may be therapeutically exploited using senolytic compounds that selectively eliminate senescent cells. Children with BRAF-V600E-driven brain tumours are commonly treated with low-dose chemotherapies and MAPK inhibitors, alone or in combination, often with good initial responses. However, a majority of patients experiences tumour regrowth following treatment withdrawal. Here, we investigate the contribution of TIS to treatment response and relapse, and assess the therapeutic potential of senolytics in preclinical models of paediatric BRAF-V600E glioma. METHODS: Preclinical models of paediatric low-grade glioma were analysed in vitro or following orthotopic transplantation into mice and treated with Dabrafenib, Trametinib, Tovorafenib or Vinblastine. Senescence was assessed using molecular and cytological markers. Sensitivity to senolytic compounds was evaluated using in vitro dose-response assays and in vivo treatment studies. RESULTS: Trametinib induced a robust senescent phenotype in BT-40 tumours in vivo, characterised by activation of a senescence-associated secretory phenotype and expansion of IBA-1-positive myeloid cells. In vitro, senescence was induced in BT-40 cells following treatment with MAPK inhibitors and Vinblastine, and was similarly observed in H9-derived human neural stem cells expressing BRAF-V600E. Navitoclax was identified as a potent senolytic against senescent BT-40 cells, primarily through inhibition of the anti-apoptotic protein BCL-xL. In vivo, combined Trametinib and Navitoclax treatment ablated senescent tumour cells, significantly reduced tumour regrowth after treatment withdrawal, and improved survival. CONCLUSIONS: These findings demonstrate that Navitoclax enhances Trametinib's anti-tumour efficacy and limits tumour relapse following treatment cessation. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Society for Neuro-Oncology. FAU - Guiho, Romain AU - Guiho R AD - Developmental Biology and Cancer Programme, Birth Defects Research Centre, Great Ormond Street Institute of Child Health, University College London, London, UK. AD - Nantes Universite, Oniris, INSERM, Regenerative Medicine and Skeleton, RMeS, UMR 1229, F-44000 Nantes, France. FAU - Hamdi, Hiba AU - Hamdi H AD - Developmental Biology and Cancer Programme, Birth Defects Research Centre, Great Ormond Street Institute of Child Health, University College London, London, UK. FAU - Cooksley, Grace AU - Cooksley G AD - Developmental Biology and Cancer Programme, Birth Defects Research Centre, Great Ormond Street Institute of Child Health, University College London, London, UK. FAU - Chang, Timoty H AU - Chang TH AD - Department of Pediatric Oncology, Dana-Farber Cancer/Boston Children's Cancer and Blood Disorders Center, Boston, MA, USA. FAU - Cao, Lei AU - Cao L AD - Developmental Biology and Cancer Programme, Birth Defects Research Centre, Great Ormond Street Institute of Child Health, University College London, London, UK. AD - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. FAU - Sigaud, Romain AU - Sigaud R AD - University Hospital Jena, Department of Pediatrics and Adolescent Medicine, Friedrich Schiller University Jena, Jena, Germany. AD - Comprehensive Cancer Center Central Germany (CCCG), Jena, Germany. FAU - Bliss, Sophie AU - Bliss S AD - Developmental Biology and Cancer Programme, Birth Defects Research Centre, Great Ormond Street Institute of Child Health, University College London, London, UK. AD - MRC Laboratory of Medical Sciences (LMS), Du Cane Road, London, W12 0NN, UK. FAU - Vardon, Ashley AU - Vardon A AD - Developmental Biology and Cancer Programme, Birth Defects Research Centre, Great Ormond Street Institute of Child Health, University College London, London, UK. AD - Institute of Cancer and Genomic Medicine, University of Birmingham, Birmingham, UK. FAU - Haston, Scott AU - Haston S AD - Developmental Biology and Cancer Programme, Birth Defects Research Centre, Great Ormond Street Institute of Child Health, University College London, London, UK. FAU - Selt, Florian AU - Selt F AD - Clinical Trial Unit (ZIPO), Department of Pediatric Hematology, Oncology, Immunology and Pulmonology, Heidelberg University Hospital, Heidelberg, Germany. AD - National Center for Tumor Diseases (NCT), Heidelberg, Germany. FAU - Bassey, Akang Leonard AU - Bassey AL AD - Developmental Biology and Cancer Programme, Birth Defects Research Centre, Great Ormond Street Institute of Child Health, University College London, London, UK. FAU - Kocher, Daniela AU - Kocher D AD - University Hospital Jena, Department of Pediatrics and Adolescent Medicine, Friedrich Schiller University Jena, Jena, Germany. AD - Comprehensive Cancer Center Central Germany (CCCG), Jena, Germany. FAU - Kedia, Ria AU - Kedia R AD - Department of Pediatric Oncology, Dana-Farber Cancer/Boston Children's Cancer and Blood Disorders Center, Boston, MA, USA. FAU - Gharai, Daniel AU - Gharai D AD - Developmental Biology and Cancer Programme, Birth Defects Research Centre, Great Ormond Street Institute of Child Health, University College London, London, UK. FAU - Gil, Jesus AU - Gil J AD - MRC Laboratory of Medical Sciences (LMS), Du Cane Road, London, W12 0NN, UK. AD - Institute of Clinical Sciences (ICS), Faculty of Medicine, Imperial College London, Du Cane Road, London W12 0NN, UK. FAU - Witt, Olaf AU - Witt O AD - Hopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany. AD - Clinical Cooperation Unit Pediatric Oncology, German Cancer Research Center Heidelberg (DKFZ), Heidelberg, Germany. FAU - Milde, Till AU - Milde T AD - University Hospital Jena, Department of Pediatrics and Adolescent Medicine, Friedrich Schiller University Jena, Jena, Germany. AD - Comprehensive Cancer Center Central Germany (CCCG), Jena, Germany. AD - Hopp Children's Cancer Center Heidelberg (KiTZ), Heidelberg, Germany. AD - Clinical Cooperation Unit Pediatric Oncology, German Cancer Research Center Heidelberg (DKFZ), Heidelberg, Germany. FAU - Hargrave, Darren AU - Hargrave D AD - Developmental Biology and Cancer Programme, Birth Defects Research Centre, Great Ormond Street Institute of Child Health, University College London, London, UK. AD - Great Ormond Street Hospital NHS Trust, London, United Kingdom. FAU - Alexandre, Paula AU - Alexandre P AD - Developmental Biology and Cancer Programme, Birth Defects Research Centre, Great Ormond Street Institute of Child Health, University College London, London, UK. FAU - Bandopadhayay, Pratiti AU - Bandopadhayay P AD - Department of Pediatric Oncology, Dana-Farber Cancer/Boston Children's Cancer and Blood Disorders Center, Boston, MA, USA. FAU - Martinez Barbera, Juan Pedro AU - Martinez Barbera JP AD - Developmental Biology and Cancer Programme, Birth Defects Research Centre, Great Ormond Street Institute of Child Health, University College London, London, UK. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Neuro Oncol JT - Neuro-oncology JID - 100887420 SB - IM OAB - Children with BRAF-V600E gliomas can respond well to treatment, but tumours may return after therapy stops. We reveal that standard treatment can push some tumour cells into a state called cellular senescence, in which they stop growing but remain alive. These senescent cells release signals that alter the tumour environment and may contribute to tumour recurrence. Importantly, we found that senescent tumour cells can be eliminated using drugs called senolytics. We show that combining standard treatment with a senolytic approach prevents tumour regrowth and significantly improves survival in mouse models. OABL- eng OTO - NOTNLM OT - BRAF-V600E driven glioma OT - BT-40 OT - MAPK inhibitors OT - Navitoclax OT - senescence EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 07:33 PHST- 2026/01/23 00:00 [received] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 07:33 [entrez] AID - 9004423 [pii] AID - 10.1093/neuonc/noag270 [doi] PST - aheadofprint SO - Neuro Oncol. 2026 Oct 29:noag270. doi: 10.1093/neuonc/noag270. PMID- 42954327 OWN - NLM STAT- Publisher LR - 20261029 IS - 1748-880X (Electronic) IS - 0007-1285 (Linking) DP - 2026 Oct 29 TI - Minimal Ablative Margin as a Key Predictor of Local Tumor Progression After Percutaneous Lung Tumor Ablation. LID - tqag260 [pii] LID - 10.1093/bjr/tqag260 [doi] AB - OBJECTIVES: Minimally invasive local therapies such as radiofrequency ablation (RFA), microwave ablation (MWA), and cryoablation (CA) are commonly used to treat malignant lung tumors. This study aimed to identify predictors of local tumor progression (LTP), focusing on the role of minimal ablative margin. METHODS: The medical records of patients >/= 20 years old with primary or metastatic lung tumors who underwent percutaneous ablation from July 2011 to December 2022 were retrospectively reviewed. Patient demographic information, tumor characteristics, and ablation details were extracted. The primary outcome was LTP, and the key variable of interest was the minimal ablative margin. RESULTS: Seventy-one patients with 88 nodules ablated were included in the analysis, and 22% of the nodules exhibited LTP. In the overall cohort, ROC analysis identified 3 mm as the optimal cut-off for minimal ablative margin to predict LTP. Nodules with margins >/= 3 mm were associated with significantly higher LTP-free survival at 5 years (82% vs. 23% margins < 3 mm). Kaplan-Meier analysis indicated a significant difference in LTP between ablative margins >/= 3 and < 3 mm (p < 0.001). RFA was associated with a higher LTP risk than MWA (hazard ratio [HR] = 5.43, 95% confidence interval [CI]: 1.84-16.05), as was a margin < 3 mm (HR = 24.24, 95% CI: 7.66-76.71). CONCLUSIONS: In this cohort, achieving an ablative margin of at least 3 mm was associated with improved LTP-free survival after lung tumor ablation. ADVANCES IN KNOWLEDGE: This threshold should be considered a pragmatic, context-dependent estimate that may require validation across ablation modalities and clinical settings. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the British Institute of Radiology. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Chang, Chao-Chun AU - Chang CC AD - Division of Thoracic Surgery, Department of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan. FAU - Hsiao, Hung-Tsung AU - Hsiao HT AD - Department of Anesthesiology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan. FAU - Chung, Ta-Jung AU - Chung TJ AD - Department of Medical Imaging, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan. FAU - Tseng, Yau-Lin AU - Tseng YL AD - Division of Thoracic Surgery, Department of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan. FAU - Yen, Yi-Ting AU - Yen YT AD - Division of Thoracic Surgery, Department of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan. AD - Division of Trauma and Acute Care Surgery, Department of Surgery, National Cheng Kung University Hospital, College of Medical College, National Cheng Kung University, Tainan, Taiwan. FAU - Lai, Wu-Wei AU - Lai WW AD - Department of Surgery, An Nan Hospital, China Medical University, Tainan, Taiwan. FAU - Lin, Chia-Ying AU - Lin CY AD - Department of Medical Imaging, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Br J Radiol JT - The British journal of radiology JID - 0373125 SB - IM OTO - NOTNLM OT - Local tumor progression (LTP) OT - cryoablation (CA) OT - lung cancer OT - microwave ablation (MWA) OT - radiofrequency ablation (RFA) EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 07:33 PHST- 2025/04/14 00:00 [received] PHST- 2026/04/06 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 07:33 [entrez] AID - 9004426 [pii] AID - 10.1093/bjr/tqag260 [doi] PST - aheadofprint SO - Br J Radiol. 2026 Oct 29:tqag260. doi: 10.1093/bjr/tqag260. PMID- 42954322 OWN - NLM STAT- Publisher LR - 20261029 IS - 1098-2787 (Electronic) IS - 0277-7037 (Linking) DP - 2026 Oct 29 TI - Mass Spectrometry Imaging-Guided Proteomics, an Emerging Frontier in Spatial Biology. LID - 10.1002/mas.70048 [doi] AB - Spatial heterogeneity is a defining feature of tissue organization, yet conventional proteomic analysis provides limited information about the spatial origins of identified proteins. Spatially guided proteomics addresses this limitation by using tissue imaging techniques to select regions of interest (ROIs) for localized analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Among the imaging modalities available for this purpose, mass spectrometry imaging (MSI) stands out for its ability to map diverse molecular species directly within tissue without predefined targets, allowing chemically distinct regions to guide tissue sampling. By bridging spatial molecular profiles with high protein coverage, MSI-guided proteomics offers comprehensive biological insight. This review examines the workflow from MSI data acquisition through ROI definition, image registration, tissue isolation, and sample preparation to LC-MS/MS analysis. We compare common MSI modalities, discuss methods for ROI isolation, and summarize advances in nanoproteomics methods that support analysis of tissue samples at the microscopic scale. We critically evaluate applications in cancer research, infectious disease, neuroscience, and environmental science. Finally, we discuss the challenges and future directions of MSI-guided proteomics. We conclude that ongoing developments in the MSI-guided proteomics workflow position it as an emerging strategy for investigating localized biological processes within heterogeneous tissues. CI - (c) 2026 The Author(s). Mass Spectrometry Reviews published by Wiley Periodicals LLC. FAU - Weke, Kenneth AU - Weke K AUID- ORCID: 0000-0001-8947-7793 AD - University of Victoria Genome BC Proteomics Centre, Victoria, British Columbia, Canada. FAU - Lum, Julian J AU - Lum JJ AUID- ORCID: 0000-0002-5624-3541 AD - School of Molecular Life Sciences, University of Victoria, Victoria, British Columbia, Canada. AD - Deeley Research Centre, BC Cancer Research Institute, Victoria, British Columbia, Canada. FAU - Goodlett, David R AU - Goodlett DR AUID- ORCID: 0000-0002-8045-8200 AD - University of Victoria Genome BC Proteomics Centre, Victoria, British Columbia, Canada. AD - School of Molecular Life Sciences, University of Victoria, Victoria, British Columbia, Canada. FAU - Petrosova, Helena AU - Petrosova H AUID- ORCID: 0009-0008-9756-7236 AD - University of Victoria Genome BC Proteomics Centre, Victoria, British Columbia, Canada. AD - School of Molecular Life Sciences, University of Victoria, Victoria, British Columbia, Canada. FAU - Dapic, Irena AU - Dapic I AUID- ORCID: 0000-0002-0716-6500 AD - Laboratory for Synthetic Methodologies in Organic Chemistry, Ruder Boskovic Institute, Division of Organic Chemistry and Biochemistry, Zagreb, Croatia. LA - eng GR - IT40873/Mitacs/ GR - 374PRO/Genome British Columbia funding for operations and technology development/ GR - 384 PRO/Genome British Columbia funding for operations and technology development/ GR - CFI-IF 45877/Canada Innovation Fund/ GR - RGPIN-2026-04712/National Science and Engineering Research Council of Canada (NSERC)/ GR - 101244235/Horizon Europe/ PT - Journal Article PT - Review DEP - 20261029 PL - United States TA - Mass Spectrom Rev JT - Mass spectrometry reviews JID - 8219702 SB - IM OTO - NOTNLM OT - laser microdissection OT - limited sample OT - liquid chromatography tandem mass spectrometry OT - spatial omics OT - spatially guided proteomics EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 07:33 PHST- 2026/10/04 00:00 [revised] PHST- 2026/04/16 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 07:33 [entrez] AID - 10.1002/mas.70048 [doi] PST - aheadofprint SO - Mass Spectrom Rev. 2026 Oct 29. doi: 10.1002/mas.70048. PMID- 42954296 OWN - NLM STAT- Publisher LR - 20261029 IS - 1549-7852 (Electronic) IS - 1040-8398 (Linking) DP - 2026 Oct 29 TI - Plant-derived exosome-like nanoparticles as candidate nano-prebiotics: from structure-enabled delivery to host-microbiota interface remodeling. PG - 1-28 LID - 10.1080/10408398.2026.2753614 [doi] AB - Gut ecosystem homeostasis depends on coordinated interactions among commensal microorganisms, the mucosal barrier, and the immune system. Conventional prebiotics rely on selective utilization by host microorganisms, but their efficacy is shaped by baseline microbiota and disease-associated ecological constraints. Plant-derived exosome-like nanoparticles (PELNs) are naturally occurring membrane-bound nanoparticles characterized by source-dependent lipid interfaces and associated molecular components, including small RNAs, proteins, polysaccharides, and plant-derived metabolites. We evaluate selected PELNs within a provisional "candidate nano-prebiotic" framework that allows particle- or component-mediated mechanisms to coexist with microbial utilization of fermentable components without implying equivalence to consensus-defined prebiotics. We critically assess evidence for microbe-directed and host-mediated effects on microbial functions, microbiota-derived metabolites, epithelial barrier integrity, and mucosal immunity, particularly in inflammatory bowel disease (IBD). Current evidence remains heterogeneous and predominantly preclinical, with major uncertainties regarding particle identity, component-specific causality, in vivo fate, microbiota dependence, dose plausibility, and mechanistic generalizability. We further define conceptual boundaries among PELNs, conventional prebiotics, and engineered nano-prebiotics and identify priorities for standardization, causal testing, food-relevant exposure assessment, safety evaluation, and mechanistically informed human studies. FAU - Huangfu, Weikang AU - Huangfu W AD - College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan, China. FAU - Liu, Cong AU - Liu C AD - College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan, China. FAU - Liu, Mengqi AU - Liu M AD - College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan, China. FAU - Sun, Hao AU - Sun H AD - College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan, China. AD - Henan Key Laboratory of Innovation and Utilization of Grassland Resources, Zhengzhou, Henan, China. AD - Henan Herbage Engineering Technology Research Center, Zhengzhou, China. FAU - Zhu, Xiaoyan AU - Zhu X AD - College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan, China. AD - Henan Key Laboratory of Innovation and Utilization of Grassland Resources, Zhengzhou, Henan, China. AD - Henan Herbage Engineering Technology Research Center, Zhengzhou, China. FAU - Li, Defeng AU - Li D AD - College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan, China. AD - Henan Key Laboratory of Innovation and Utilization of Grassland Resources, Zhengzhou, Henan, China. AD - Henan Herbage Engineering Technology Research Center, Zhengzhou, China. FAU - Cui, Yalei AU - Cui Y AD - College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan, China. AD - Henan Key Laboratory of Innovation and Utilization of Grassland Resources, Zhengzhou, Henan, China. AD - Henan Herbage Engineering Technology Research Center, Zhengzhou, China. FAU - Wang, Zhichang AU - Wang Z AD - College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan, China. AD - Henan Key Laboratory of Innovation and Utilization of Grassland Resources, Zhengzhou, Henan, China. AD - Henan Herbage Engineering Technology Research Center, Zhengzhou, China. FAU - Liu, Boshuai AU - Liu B AD - College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan, China. AD - Henan Key Laboratory of Innovation and Utilization of Grassland Resources, Zhengzhou, Henan, China. AD - Henan Herbage Engineering Technology Research Center, Zhengzhou, China. FAU - Shi, Yinghua AU - Shi Y AD - College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, Henan, China. AD - Henan Key Laboratory of Innovation and Utilization of Grassland Resources, Zhengzhou, Henan, China. AD - Henan Herbage Engineering Technology Research Center, Zhengzhou, China. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - United States TA - Crit Rev Food Sci Nutr JT - Critical reviews in food science and nutrition JID - 8914818 SB - IM OTO - NOTNLM OT - Plant-derived exosome-like nanoparticles OT - host-microbiota interface OT - inflammatory bowel disease OT - nano-prebiotics OT - precision microbiota modulation EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 07:23 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 07:23 [entrez] AID - 10.1080/10408398.2026.2753614 [doi] PST - aheadofprint SO - Crit Rev Food Sci Nutr. 2026 Oct 29:1-28. doi: 10.1080/10408398.2026.2753614. PMID- 42954286 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1464-5157 (Electronic) IS - 0265-6736 (Linking) VI - 43 IP - 1 DP - 2026 Dec TI - High-intensity focused ultrasound as a bridging vascular-modulating therapy for fertility-sparing management of placental site trophoblastic tumor complicated by uterine arteriovenous fistula: a case report. PG - 2750688 LID - 10.1080/02656736.2026.2750688 [doi] AB - BACKGROUND: Placental site trophoblastic tumor (PSTT) is a rare gestational trophoblastic neoplasm characterized by local invasiveness and marked vascularity. Surgical management may be associated with substantial bleeding risk and loss of fertility, particularly when complicated by uterine arteriovenous fistula (UAVF). High-intensity focused ultrasound (HIFU) may provide a fertility-preserving adjunctive approach in selected cases. CASE PRESENTATION: A 23-year-old woman presented with persistently plateaued serum human chorionic gonadotropin levels following hysteroscopic management for suspected incomplete abortion. Imaging revealed a deeply invasive hypervascular uterine lesion with UAVF, raising strong suspicion of gestational trophoblastic neoplasia. Given her strong desire for fertility preservation and high surgical risk, ultrasound-guided HIFU was performed as an initial vascular-modulating intervention. Subsequent expert pathological review confirmed PSTT. RESULTS: HIFU achieved significant reduction in lesion perfusion and vascular flow, as confirmed by Doppler ultrasound and contrast-enhanced MRI. Although complete tumor ablation was not achieved due to lesion size and deep invasion, subsequent low-dose chemotherapy resulted in complete biochemical remission accompanied by radiologic regression of residual lesions. No major HIFU-related complications were observed, and normal menstrual function resumed during follow-up. CONCLUSION: HIFU is a technically feasible and safe fertility-preserving adjunct for vascular modulation in hypervascular PSTT complicated by UAVF. While HIFU alone may not achieve definitive oncologic control in large or deeply invasive lesions, it can serve as a bridging strategy to reduce tumor perfusion and vascularity, facilitating subsequent systemic therapy in carefully selected patients. This approach demonstrates the potential role of HIFU as a bridging vascular-modulating strategy within multimodal fertility-preserving management. FAU - Liang, Yufang AU - Liang Y AD - Shanxi Key Laboratory of Cervical Disease Research, Jincheng People's Hospital, Jincheng, China. AD - Obstetrics and Gynecology Medical Center, Jincheng Hospital Affiliated to Changzhi Medical College, Jincheng People's Hospital, Shanxi, China. FAU - Wei, Yini AU - Wei Y AD - Shanxi Key Laboratory of Cervical Disease Research, Jincheng People's Hospital, Jincheng, China. AD - Obstetrics and Gynecology Medical Center, Jincheng Hospital Affiliated to Changzhi Medical College, Jincheng People's Hospital, Shanxi, China. FAU - Jing, Fangfang AU - Jing F AD - Shanxi Key Laboratory of Cervical Disease Research, Jincheng People's Hospital, Jincheng, China. AD - Obstetrics and Gynecology Medical Center, Jincheng Hospital Affiliated to Changzhi Medical College, Jincheng People's Hospital, Shanxi, China. FAU - Zou, Yun AU - Zou Y AD - Shanxi Key Laboratory of Cervical Disease Research, Jincheng People's Hospital, Jincheng, China. AD - Obstetrics and Gynecology Medical Center, Jincheng Hospital Affiliated to Changzhi Medical College, Jincheng People's Hospital, Shanxi, China. FAU - Xiao, Yujiao AU - Xiao Y AD - Department of Pathology, Jincheng Hospital Affiliated to Changzhi Medical College, Jincheng People's Hospital, Shanxi, China. FAU - Wang, Zeyu AU - Wang Z AD - Shanxi Key Laboratory of Cervical Disease Research, Jincheng People's Hospital, Jincheng, China. AD - Obstetrics and Gynecology Medical Center, Jincheng Hospital Affiliated to Changzhi Medical College, Jincheng People's Hospital, Shanxi, China. FAU - Li, Lei AU - Li L AD - Shanxi Key Laboratory of Cervical Disease Research, Jincheng People's Hospital, Jincheng, China. AD - Obstetrics and Gynecology Medical Center, Jincheng Hospital Affiliated to Changzhi Medical College, Jincheng People's Hospital, Shanxi, China. FAU - Meng, Ru AU - Meng R AD - Shanxi Key Laboratory of Cervical Disease Research, Jincheng People's Hospital, Jincheng, China. AD - Obstetrics and Gynecology Medical Center, Jincheng Hospital Affiliated to Changzhi Medical College, Jincheng People's Hospital, Shanxi, China. FAU - Chang, Jinjun AU - Chang J AD - Shanxi Key Laboratory of Cervical Disease Research, Jincheng People's Hospital, Jincheng, China. FAU - Li, Fang AU - Li F AD - Shanxi Key Laboratory of Cervical Disease Research, Jincheng People's Hospital, Jincheng, China. AD - Obstetrics and Gynecology Medical Center, Jincheng Hospital Affiliated to Changzhi Medical College, Jincheng People's Hospital, Shanxi, China. LA - eng PT - Case Reports PT - Journal Article DEP - 20261029 PL - England TA - Int J Hyperthermia JT - International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group JID - 8508395 SB - IM MH - Humans MH - Female MH - *Trophoblastic Tumor, Placental Site/complications/pathology/therapy/diagnostic imaging MH - Pregnancy MH - *High-Intensity Focused Ultrasound Ablation/methods MH - Young Adult MH - *Arteriovenous Fistula/complications/therapy MH - *Fertility Preservation/methods OTO - NOTNLM OT - Fertility preservation OT - bridging therapy OT - high-intensity focused ultrasound OT - placental site trophoblastic tumor OT - uterine arteriovenous fistula EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 07:23 PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 07:23 [entrez] AID - 10.1080/02656736.2026.2750688 [doi] PST - ppublish SO - Int J Hyperthermia. 2026 Dec;43(1):2750688. doi: 10.1080/02656736.2026.2750688. Epub 2026 Oct 29. PMID- 42954265 OWN - NLM STAT- Publisher LR - 20261029 IS - 1532-2297 (Electronic) IS - 1082-6068 (Linking) DP - 2026 Oct 29 TI - Production and biochemical characterization of an Aspergillus oryzae protease preparation using dairy whey under submerged fermentation and evaluation of its milk-clotting activity. PG - 1-11 LID - 10.1080/10826068.2026.2749458 [doi] AB - Proteases are among the most commercially important industrial enzymes due to their broad applications in food, pharmaceutical, detergent, and biotechnological processes. In this context, bovine dairy whey, a major by-product of the dairy industry, represents a promising low-cost substrate for sustainable enzyme production. This study aimed to produce, purify, and biochemically characterize a protease from Aspergillus oryzae cultivated under submerged fermentation using bovine dairy whey as substrate. Initially, A. aculeatus, A. ochraceus, and A. oryzae were evaluated for proteolytic activity. The selected enzyme was pre-purified by ammonium sulfate precipitation, ion-exchange chromatography, and Superdex G-200 gel filtration, followed by biochemical characterization. Aspergillus oryzae exhibited the highest proteolytic activity (96 U/mL). The enzyme achieved a purification fold of 56.63 and specific activity of 84.38 U/mg. Maximum activity was observed at pH 5.0 and 50 degrees C, whereas greater thermal stability occurred at 40 degrees C. PMSF and EDTA strongly inhibited enzymatic activity. The inhibition by PMSF suggests the possible involvement of serine residues in the catalytic activity, thus, consistent with a serine protease. Mn(2+) and Fe(2+) maintained residual activities above 80%, whereas Cu(2+) caused the strongest inhibition. Additionally, the partially purified enzyme showed expressive milk-clotting activity, reaching 75.94 MCU and 91.60% coagulation after gel filtration chromatography. These findings highlight the biotechnological potential of A. oryzae proteases produced from bovine dairy whey for sustainable enzyme production and milk-clotting applications within circular economy approaches. FAU - Silva, Eloisa Maria Souto AU - Silva EMS AD - Integrated Multiuser Laboratory in Applied Biotechnology-LIBAS, Institute of Biological Sciences, University of Pernambuco (UPE), Recife, Brazil. FAU - Ramos, Diego Gomes AU - Ramos DG AD - Integrated Multiuser Laboratory in Applied Biotechnology-LIBAS, Institute of Biological Sciences, University of Pernambuco (UPE), Recife, Brazil. AD - Multiuser Biotechnology Laboratory of the Pernambuco Sertao, University of Pernambuco-UPE, Arcoverde, Brazil. FAU - Barbosa Cardoso, Kethylen Barbara AU - Barbosa Cardoso KB AD - Integrated Multiuser Laboratory in Applied Biotechnology-LIBAS, Institute of Biological Sciences, University of Pernambuco (UPE), Recife, Brazil. FAU - Souza Lima, Barbara Cibele AU - Souza Lima BC AD - Integrated Multiuser Laboratory in Applied Biotechnology-LIBAS, Institute of Biological Sciences, University of Pernambuco (UPE), Recife, Brazil. FAU - Lima Duarte, Carolina de Albuquerque AU - Lima Duarte CA AD - Multiuser Biotechnology Laboratory of the Pernambuco Sertao, University of Pernambuco-UPE, Arcoverde, Brazil. FAU - Nascimento, Thiago Pajeu AU - Nascimento TP AD - Campus Professor Cinobelina Elvas, Federal University of Piaui, Teresina, Brazil. FAU - Silveira Filho, Vladimir da Mota AU - Silveira Filho VDM AD - Campus Garanhuns, University of Pernambuco, Brazil. FAU - Costa, Romero Marcos Pedrosa Brandao AU - Costa RMPB AD - Integrated Multiuser Laboratory in Applied Biotechnology-LIBAS, Institute of Biological Sciences, University of Pernambuco (UPE), Recife, Brazil. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Prep Biochem Biotechnol JT - Preparative biochemistry & biotechnology JID - 9607037 SB - IM OTO - NOTNLM OT - Biorefinery OT - dairy whey OT - milk-coagulating OT - protease OT - residues EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 07:12 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 07:12 [entrez] AID - 10.1080/10826068.2026.2749458 [doi] PST - aheadofprint SO - Prep Biochem Biotechnol. 2026 Oct 29:1-11. doi: 10.1080/10826068.2026.2749458. PMID- 42954261 OWN - NLM STAT- Publisher LR - 20261029 IS - 1477-2639 (Electronic) IS - 1025-6016 (Linking) DP - 2026 Oct 29 TI - Stable isotopes indicate consistent trophic structures but marked spatial variation in isotopic values across contrasting high-altitude Andean salars. PG - 1-26 LID - 10.1080/10256016.2026.2742438 [doi] AB - High-altitude Andean salars are poly-extreme wetlands that support communities dominated by microorganisms and important populations of flamingos. Much of the work on these systems has focused on microbial community composition and function, but little is known regarding their trophic ecology. We evaluated the use of stable isotopes to compare food web organisation and consumption pathways in three contrasting Andean salars. We analysed delta(13)C, delta(15)N and delta(34)S of primary producers, aquatic invertebrates and flamingos (feathers and faeces) in Laguna Puilar (Salar de Atacama), Salar de Tara and Salar de Pujsa. We quantified the isotopic niche volume and overlap between trophic components and used Bayesian mixing models to estimate the relative contribution of putative prey resources to flamingo diet. The trophic organisation was quite similar among the salars, however, the relative size of community and consumer isotopic niches differed markedly. At Puilar and Tara, most invertebrate niches were narrow and largely nested within the isotopic space occupied by flamingos, suggesting restricted resource use and limited dispersion in three-isotope space. In contrast, Pujsa showed a broader and more heterogeneous occupation of isotopic space among consumer taxa. Mixing models indicated that one or two dominant groups of aquatic invertebrates, together with diatoms, contributed substantially to the flamingos' diet, consistent with previous studies. The isotopic values of the feathers showed high variability, consistent with integration between multiple wetlands and, potentially, multiple species of flamingos, rather than simple local trophic generalism. Despite limitations related to the inability to assign feathers to specific species and incomplete coverage of meiofauna, the triple isotope approaches provided robust information on dominant energy sources and revealed local 'trophic identities' among salars. Stable isotopes therefore offer a non-invasive and effective framework for monitoring trophic structure in remote and difficult to study Andean salars under increasing anthropogenic pressure. FAU - Salazar, Gonzalo AU - Salazar G AUID- ORCID: 0009-0000-8847-075X AD - Doctorado en Ciencias Aplicadas mencion Sistemas Acuaticos, Facultad de Ciencias del Mar y Recursos Biologicos, Universidad de Antofagasta, Antofagasta, Chile. FAU - Aguilar, Pablo AU - Aguilar P AUID- ORCID: 0000-0001-7913-7963 AD - Departamento de Biotecnologia, Facultad de Ciencias del Mar y de Recursos Biologicos, Universidad de Antofagasta, Antofagasta, Chile. AD - Laboratorio de Complejidad Microbiana, Instituto Antofagasta, Universidad de Antofagasta, Antofagasta, Chile. AD - Centre for Biotechnology & Bioengineering (CeBiB), Santiago, Chile. FAU - Sacco, Mattia AU - Sacco M AUID- ORCID: 0000-0001-6535-764X AD - Subterranean Research and Groundwater Ecology (SuRGE) Group, Trace and Environmental DNA (TrEnD) Lab, School of Molecular and Life Sciences, Curtin University, Perth, Australia. AD - Laboratoire de Biologie des Organismes et des Ecosystemes Aquatiques-BOREA, Museum National d'Histoire Naturelle, SU, CNRS, IRD, UA, Paris, France. AD - Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy. FAU - Harrod, Chris AU - Harrod C AUID- ORCID: 0000-0002-5353-1556 AD - Scottish Centre for Ecology and the Natural Environment, School of Biodiversity, One Health and Veterinary Medicine, University of Glasgow, Glasgow, UK. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Isotopes Environ Health Stud JT - Isotopes in environmental and health studies JID - 9602611 SB - IM OTO - NOTNLM OT - Altiplano OT - Atacama desert OT - Bayesian mixing models OT - Northern Chile OT - aquatic invertebrates OT - food webs OT - isotopic niche EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 07:03 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 07:03 [entrez] AID - 10.1080/10256016.2026.2742438 [doi] PST - aheadofprint SO - Isotopes Environ Health Stud. 2026 Oct 29:1-26. doi: 10.1080/10256016.2026.2742438. PMID- 42954240 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1365-2834 (Electronic) IS - 0966-0429 (Linking) VI - 2026 IP - 1 DP - 2026 TI - Reducing Noise and Alarm Burden in Adult Intensive Care Units: An Integrative Review of Interventions and Implications for Nursing Management. LID - e9617407 [pii] LID - 10.1155/jonm/9617407 [doi] AB - AIM: To synthesise evidence on noise-reduction and alarm-management strategies in adult intensive care units and examine their implications for nursing management. BACKGROUND: ICU noise and alarm burden present challenges for patient rest, clinical surveillance, nursing workflow and staff experience. Intervention effectiveness and sustainability remain uncertain. EVALUATION: An integrative review guided by Whittemore and Knafl's framework synthesised empirical, implementation and review-level evidence identified through PubMed, Scopus, CINAHL and reference screening. KEY ISSUES: Interventions addressed alarm management, environmental modification, quiet-hour practices, staff engagement and multicomponent implementation. Reported benefits varied across studies, and available evidence did not establish the superiority of bundled approaches. Direct evidence concerning workforce well-being, nursing leadership, clinical surveillance and sustained improvement was limited. CONCLUSION: Quiet ICU interventions may improve selected acoustic and alarm-related outcomes, but their comparative effectiveness and long-term sustainability remain uncertain. IMPLICATIONS FOR NURSING MANAGEMENT: Nurse leaders may consider context-sensitive interventions supported by alarm governance, workflow integration, staff participation and ongoing evaluation. Future studies should assess nursing workload, clinical surveillance, patient safety and sustainability alongside acoustic outcomes. CI - Copyright (c) 2026 Wei Jun Dan Ong et al. Journal of Nursing Management published by John Wiley & Sons Ltd. FAU - Ong, Wei Jun Dan AU - Ong WJD AUID- ORCID: 0000-0003-4055-5222 AD - Respiratory Therapy Department, Ng Teng Fong General Hospital, National University Health System, Singapore, nuhs.edu.sg. AD - Department of Health Sciences, College of Health Sciences, Rush University, Chicago, Illinois, USA, rush.edu. FAU - Vidanes, Michael Camba AU - Vidanes MC AD - Respiratory Therapy Department, Ng Teng Fong General Hospital, National University Health System, Singapore, nuhs.edu.sg. FAU - Chong, Woon Hean Keenan AU - Chong WHK AUID- ORCID: 0000-0002-2070-3585 AD - Department of Intensive Care Medicine, Ng Teng Fong General Hospital, National University Health System, Singapore, nuhs.edu.sg. FAU - Khan, Faheem Ahmed AU - Khan FA AD - Department of Intensive Care Medicine, Ng Teng Fong General Hospital, National University Health System, Singapore, nuhs.edu.sg. FAU - Leong, Patricia AU - Leong P AD - Nursing Administration, Ng Teng Fong General Hospital, National University Health System, Singapore, nuhs.edu.sg. FAU - Azul, Lawrence Ace Matunan AU - Azul LAM AUID- ORCID: 0009-0003-5516-7107 AD - Respiratory Therapy Department, Ng Teng Fong General Hospital, National University Health System, Singapore, nuhs.edu.sg. FAU - Yap, Adrian Ujin AU - Yap AU AUID- ORCID: 0000-0003-0361-6209 AD - Clinical Research Unit, Ng Teng Fong General Hospital, National University Health System, Singapore, nuhs.edu.sg. AD - National Dental Research Institute Singapore, National Dental Centre Singapore, and Duke-NUS Medical School, Singapore Health Services, Singapore, singhealth.com.sg. LA - eng PT - Journal Article PT - Review PL - England TA - J Nurs Manag JT - Journal of nursing management JID - 9306050 SB - IM MH - Humans MH - Intensive Care Units/organization & administration/statistics & numerical data/standards MH - *Noise/adverse effects/prevention & control MH - *Clinical Alarms/standards/adverse effects MH - Working Conditions OTO - NOTNLM OT - clinical alarms OT - intensive care units OT - noise OT - nursing management OT - patient safety EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:53 PHST- 2026/10/10 00:00 [revised] PHST- 2026/05/05 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:53 [entrez] AID - e9617407 [pii] AID - 10.1155/jonm/9617407 [doi] PST - ppublish SO - J Nurs Manag. 2026;2026(1):e9617407. doi: 10.1155/jonm/9617407. PMID- 42954236 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 0392-856X (Print) IS - 0392-856X (Linking) VI - 44 IP - 10 DP - 2026 Oct TI - Exploring the faecal miRNA profile in familial Mediterranean fever patients and the role of miR-21-5p in inflammatory pathways associated with FMF pathogenesis. PG - 2114-2123 LID - 10.55563/clinexprheumatol/oj2iy9 [doi] AB - OBJECTIVES: Beyond MEFV mutations, epigenetics seems to play an important role in the manifestation of familial Mediterranean fever (FMF). This study aimed to evaluate the differential expression of selected miRNAs in stool samples from FMF patients, and to investigate the functional role of miR-21-5p in FMF-related inflammatory pathways. METHODS: Thirty-four FMF patients, 15 patients with Clostridioides difficile infection (CDI), and 25 healthy controls were enrolled in this study. The faecal levels of 27 miRNAs associated with inflammation, apoptosis, and autophagy were quantified by qRT-PCR. The functional role of miR-21-5p in regulating NF-kB and pyrin inflammasome pathways was investigated in LPS-stimulated THP-1 derived macrophages transfected with an anti-mir-21-5p inhibitor via western blot. RESULTS: Among the 27 analysed miRNAs, eight were significantly upregulated in FMF patients compared with controls, with miR-21-5p, miR-30e-3p, and miR-148a-3p uniquely elevated in FMF. Downregulation of the anti-inflammatory miR-186-5p and miR-374b-5p was observed particularly in M694V homozygous patients, while let-7d-5p, and miR-148b-3p were associated with higher disease severity. Functional inhibition of miR-21-5p in LPS-stimulated THP-1-derived macrophages reduced NF-kB activation and decreased the expression of inflammasome-associated proteins except ASC, consistent with ASC-independent modulation of inflammasome signalling, potentially through direct caspase-1 activation. CONCLUSIONS: Our findings highlight the role of miRNAs in intestinal inflammation and their potential as diagnostic and prognostic biomarkers in FMF. Our functional data underscore the regulatory role of miR-21-5p in FMF-related inflammatory pathways and warrant further studies to elucidate its therapeutic potential, particularly in patients refractory to colchicine or as an adjunct to existing treatments. FAU - Chaaban, Ahlam AU - Chaaban A AD - Department of Biological Sciences, School of Arts and Sciences, Lebanese American University (LAU), Beirut, Lebanon. FAU - Skaineh, Zeinab AU - Skaineh Z AD - Department of Biological Sciences, School of Arts and Sciences, Lebanese American University (LAU), Beirut, Lebanon. FAU - Hammoud, Razane AU - Hammoud R AD - Department of Biological Sciences, School of Arts and Sciences, Lebanese American University (LAU), Beirut, Lebanon. FAU - Chebly, Alain AU - Chebly A AD - Center Jacques Loiselet for Medical Genetics and Genomics (CGGM), Faculty of Medicine, Saint Joseph University of Beirut (USJ), Beirut, Lebanon. FAU - Karam, Louna AU - Karam L AD - Department of Biological Sciences, School of Arts and Sciences, Lebanese American University (LAU), Beirut, Lebanon. FAU - Eldawra, Eliana AU - Eldawra E AD - Department of Biological Sciences, School of Arts and Sciences, Lebanese American University (LAU), Beirut, Lebanon. FAU - Ibrahim, Jose-Noel AU - Ibrahim JN AD - Department of Biological Sciences, School of Arts and Sciences, Lebanese American University (LAU), Beirut, Lebanon. josenoel.ibrahim@lau.edu.lb. LA - eng PT - Journal Article DEP - 20261026 PL - Italy TA - Clin Exp Rheumatol JT - Clinical and experimental rheumatology JID - 8308521 RN - 0 (MicroRNAs) RN - 0 (MIRN21 microRNA, human) RN - 0 (Pyrin) RN - 0 (NF-kappa B) RN - 0 (Inflammasomes) SB - IM MH - Humans MH - *Familial Mediterranean Fever/genetics/metabolism/diagnosis MH - *MicroRNAs/genetics/metabolism MH - Female MH - Male MH - Adult MH - *Feces/chemistry MH - Signal Transduction MH - Case-Control Studies MH - THP-1 Cells MH - Pyrin/metabolism/genetics MH - NF-kappa B/metabolism MH - Young Adult MH - Inflammasomes/metabolism MH - Adolescent MH - Inflammation/genetics MH - Apoptosis MH - Macrophages/metabolism MH - Autophagy EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:52 PHST- 2026/04/07 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:52 [entrez] AID - 23931 [pii] AID - 10.55563/clinexprheumatol/oj2iy9 [doi] PST - ppublish SO - Clin Exp Rheumatol. 2026 Oct;44(10):2114-2123. doi: 10.55563/clinexprheumatol/oj2iy9. Epub 2026 Oct 26. PMID- 42954226 OWN - NLM STAT- Publisher LR - 20261029 IS - 1538-0254 (Electronic) IS - 0739-1102 (Linking) DP - 2026 Oct 29 TI - iDHSs-HO: a high-order correlation method for identifying DNase I hypersensitive sites. PG - 1-13 LID - 10.1080/07391102.2026.2752079 [doi] AB - DNase I hypersensitivity sites (DHSs) are critical elements in DNA regulatory mechanisms, and their accurate identification holds substantial significance for biomedical research. While traditional experimental methods can identify DHSs, they are often hindered by high costs and time demands. With the progress of machine learning, computational approaches have drawn growing interest. In this work, we introduce a machine learning framework that leverages dinucleotide physicochemical properties to recognize DHSs. To capture comprehensive correlation information, a sliding window strategy was implemented. Moreover, two distinct techniques-clustering and principal component analysis-were utilized to derive high-order correlation characteristics. To minimize the impact of redundant and noisy information, the F-score method was applied for feature selections. Subsequently, the selected features were fed into a support vector machine to distinguish DHSs from non-DHSs. Experimental outcomes demonstrated that the clustering-based approach obtained superior performance, with classification accuracies 0.9902 and 0.9896 on two independent datasets. Compared with earlier studies using the same datasets, our proposed method also yielded improved results. This suggests that our model is effective for DHSs identification. For academic purposes, we provide the data and codes used in current work at https://figshare.com/articles/online_resource/iDHSs-HO/31860025. FAU - Chen, Yan AU - Chen Y AD - School of Information Engineering, Jiangxi Science and Technology Normal University, Nanchang, China. FAU - Zou, Hongliang AU - Zou H AD - School of Information Engineering, Jiangxi Science and Technology Normal University, Nanchang, China. AD - School of Artificial Intelligence, Jiangxi Science and Technology Normal University, Nanchang, China. AD - Jiangxi Engineering Research Center of Unattended Perception System and Artificial Intelligence Technology, Jiangxi Science and Technology Normal University, Nanchang, China. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - J Biomol Struct Dyn JT - Journal of biomolecular structure & dynamics JID - 8404176 SB - IM OTO - NOTNLM OT - DNase I hypersensitive sites OT - F-score OT - high-order correlation OT - physicochemical properties OT - support vector machine EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:44 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:44 [entrez] AID - 10.1080/07391102.2026.2752079 [doi] PST - aheadofprint SO - J Biomol Struct Dyn. 2026 Oct 29:1-13. doi: 10.1080/07391102.2026.2752079. PMID- 42954204 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 0869-866X (Print) IS - 0869-866X (Linking) VI - 34 IP - 5 DP - 2026 Dec 15 TI - [The assessment of the level and perspectives of digitization of regional markets of medical services in Russia]. PG - 1223-1229 LID - 10.32687/0869-866X-2026-34-5-1223-1229 [doi] AB - The article considers issues of digitization of regional markets of medical services in the Russian Federation. The concept of market of medical cervices, its subject object structure and bargain items are revealed. It is demonstrated that regional markets of medical services are characterized by profound differentiation conditioned by economical, demographic, infrastructural and institutional factors. on the basis of analysis of statistical data the typologization of regions is carried out according level of development and structure of markets and degree of their digitization. The four groups of regions are selected: leaders in digitization, "overtaking" regions with higher potential, regions with average level of digitization and backward regions (outsiders). For each group typical strategies of digital development with differentiation of priorities (telemedicine, EMIAS (Unified Medical Information and Analytical System), AI diagnostic, routing) are proposed. The necessity of partnership between of the state and private medical business is substantiated. The state organizes The infrastructure and standards are organized by the state and innovations and adaptable services by private sector. The risks of fragmentation and digital inequality, etc. are established. FAU - Bagautdinova, N G AU - Bagautdinova NG AD - The Federal State Autonomous Educational Institution of Higher Education "The Kazan (Volga Region) Federal University", 420008, Kazan, Russia. FAU - Vertakova, Y V AU - Vertakova YV AD - The Federal State Autonomous Educational Institution of Higher Education "The Russian State Humanitarian University", 125993, Moscow, Russia. FAU - Plotnikov, V A AU - Plotnikov VA AD - The Federal State Autonomous Educational Institution of Higher Education "The St. Petersburg State Economic University", 191023, St. Petersburg, Russia. FAU - Hajrullin, I A AU - Hajrullin IA AD - The Federal State Autonomous Educational Institution of Higher Education "The Kazan (Volga Region) Federal University", 420008, Kazan, Russia. LA - rus PT - English Abstract PT - Journal Article DEP - 20261215 PL - Russia (Federation) TA - Probl Sotsialnoi Gig Zdravookhranenniiai Istor Med JT - Problemy sotsial'noi gigieny, zdravookhraneniia i istorii meditsiny JID - 101270373 SB - IM MH - Russia MH - Humans MH - *Telemedicine/organization & administration MH - *Delivery of Health Care/organization & administration OTO - NOTNLM OT - differentiation OT - digital maturity OT - digitization OT - health care OT - medical care OT - medical services OT - public-private partnership. OT - regional market OT - telemedicine EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:44 PHST- 2026/10/27 00:00 [received] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:44 [entrez] AID - 10.32687/0869-866X-2026-34-5-1223-1229 [doi] PST - epublish SO - Probl Sotsialnoi Gig Zdravookhranenniiai Istor Med. 2026 Dec 15;34(5):1223-1229. doi: 10.32687/0869-866X-2026-34-5-1223-1229. PMID- 42954191 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1365-2834 (Electronic) IS - 0966-0429 (Linking) VI - 2026 IP - 1 DP - 2026 TI - "Growth From Failure": A Qualitative Study of NICU Nurses' Psychological Experiences Following Failed Neonatal Resuscitation. LID - e2056428 [pii] LID - 10.1155/jonm/2056428 [doi] AB - AIM: This study investigated the emotional responses and underlying psychological processes experienced by nurses in the neonatal intensive care unit (NICU) following unsuccessful neonatal resuscitation. BACKGROUND: Nurses, particularly neonatal nurses, experience substantial psychological pressure and multifaceted challenges in the aftermath of resuscitation failure. Although existing literature has examined nurses' emotional responses to neonatal death, a significant gap persists in understanding the distinct psychological experiences of neonatal nurses following unsuccessful resuscitation attempts. METHODS: This was a descriptive phenomenological study. Purposive sampling was employed to recruit 14 NICU nurses from a tertiary hospital in Chengdu, Sichuan Province. Participants were selected based on their firsthand experiences of neonatal resuscitation failure and participated in semistructured, face-to-face interviews. The interviews were audio-recorded, transcribed verbatim, and analyzed using Colaizzi's seven-step method. RESULTS: NICU nurses described their personal experiences following failed neonatal resuscitation. Four themes emerged concerning nurses' experiences and growth following resuscitation failure: (1) physical and mental exhaustion; (2) communication challenges; (3) reflection and support needs; and (4) achievements and growth. CONCLUSION: This study revealed the complex psychological experiences of NICU nurses following resuscitation failure. These findings highlight opportunities to support nurses' professional growth and improve team culture and care systems. Consequently, establishing a multidimensional support system is important to support nurses involved in neonatal resuscitation. IMPLICATIONS FOR NURSING MANAGEMENT: Nurse managers and hospital administrators should establish a comprehensive, multidimensional support system to improve nursing competencies. This system should prioritize standardized training in the theoretical knowledge and practical skills needed for emergency care, while also strengthening nurses' capacity to provide humanistic care. CI - Copyright (c) 2026 Xushu Chen et al. Journal of Nursing Management published by John Wiley & Sons Ltd. FAU - Chen, Xushu AU - Chen X AUID- ORCID: 0009-0004-3195-9414 AD - Department of Neonatology Nursing, West China Second University Hospital, Sichuan University, Chengdu, China, scu.edu.cn. AD - Key Laboratory of Birth Defects and Related Diseases of Women and Children, Sichuan University, Ministry of Education, Chengdu, 610041, China, meb.gov.tr. FAU - Yang, Ru AU - Yang R AUID- ORCID: 0009-0007-6783-464X AD - Department of Neonatology Nursing, West China Second University Hospital, Sichuan University, Chengdu, China, scu.edu.cn. AD - Key Laboratory of Birth Defects and Related Diseases of Women and Children, Sichuan University, Ministry of Education, Chengdu, 610041, China, meb.gov.tr. FAU - Shi, Zeyao AU - Shi Z AUID- ORCID: 0009-0008-8193-3502 AD - Department of Neonatology Nursing, West China Second University Hospital, Sichuan University, Chengdu, China, scu.edu.cn. AD - Key Laboratory of Birth Defects and Related Diseases of Women and Children, Sichuan University, Ministry of Education, Chengdu, 610041, China, meb.gov.tr. FAU - Hou, Shulin AU - Hou S AD - Department of Neonatology Nursing, West China Second University Hospital, Sichuan University, Chengdu, China, scu.edu.cn. AD - Key Laboratory of Birth Defects and Related Diseases of Women and Children, Sichuan University, Ministry of Education, Chengdu, 610041, China, meb.gov.tr. FAU - Yan, Jing AU - Yan J AD - Department of Neonatology Nursing, West China Second University Hospital, Sichuan University, Chengdu, China, scu.edu.cn. AD - Key Laboratory of Birth Defects and Related Diseases of Women and Children, Sichuan University, Ministry of Education, Chengdu, 610041, China, meb.gov.tr. FAU - Li, Xiaowen AU - Li X AD - Department of Neonatology Nursing, West China Second University Hospital, Sichuan University, Chengdu, China, scu.edu.cn. AD - Key Laboratory of Birth Defects and Related Diseases of Women and Children, Sichuan University, Ministry of Education, Chengdu, 610041, China, meb.gov.tr. LA - eng PT - Journal Article PL - England TA - J Nurs Manag JT - Journal of nursing management JID - 9306050 SB - IM MH - Humans MH - Qualitative Research MH - Intensive Care Units, Neonatal/organization & administration/statistics & numerical data MH - *Resuscitation/psychology/nursing MH - Female MH - Adult MH - Male MH - China MH - *Nurses/psychology MH - Psychological Growth MH - Infant, Newborn OTO - NOTNLM OT - NICU OT - nurses OT - psychological experience OT - qualitative research OT - resuscitation failure EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:43 PHST- 2026/10/09 00:00 [revised] PHST- 2025/04/27 00:00 [received] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:43 [entrez] AID - e2056428 [pii] AID - 10.1155/jonm/2056428 [doi] PST - ppublish SO - J Nurs Manag. 2026;2026(1):e2056428. doi: 10.1155/jonm/2056428. PMID- 42954179 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1864-564X (Electronic) IS - 1864-5631 (Linking) VI - 19 IP - 21 DP - 2026 Nov 12 TI - Electrode Architectures in Biophotoelectrochemical Systems for Sustainable Energy and Environmental Applications. LID - e71162 [pii] LID - 10.1002/cssc.71162 [doi] AB - Current energy demand and the need for environmental stewardship call for transformative technologies that can simultaneously produce clean energy and remediate waste. Biophotoelectrochemical (BPEC) systems are promising platforms that couple the light-harvesting capability of semiconductor photocatalysts with the catalytic precision of biocatalysts using an electrically addressable electrode. BPEC systems provide tunable reaction pathways, improved scalability, easier product handling, and flexibility in coupling various reactions by modifying the electrode architecture. This review presents a comprehensive discussion of these architectures in BPEC systems, beginning with the fundamental principles of how photoelectrodes and bioelectrodes work. This review then provides a systematic examination of two different electrode architectures: separated electrodes (photoelectrode | bioelectrode) and biohybrid photoelectrodes (biophotoelectrodes). The discussion covers their configurations, working principles, and representative applications such as H(2) evolution, CO(2) reduction, N(2) fixation, pollutant removal, and energy recovery. The review also summarizes key performance metrics and current research trends. By integrating the complementary strengths of photoelectrodes and bioelectrodes, BPEC systems can overcome individual technological limitations and support sustainable energy and environmental applications through self-biased operation and enhanced product selectivity. Finally, the review discusses the challenges and future directions of advancing BPEC systems into scalable, multifunctional platforms for next-generation sustainable technologies. CI - (c) 2026 The Author(s). ChemSusChem published by Wiley-VCH GmbH. FAU - Budiarso, Indra J AU - Budiarso IJ AUID- ORCID: 0000-0002-0792-5706 AD - Graduate School of Environmental Studies, Tohoku University, Sendai, Miyagi, Japan. FAU - Umetsu, Masaki AU - Umetsu M AUID- ORCID: 0000-0003-0102-9748 AD - Graduate School of Environmental Studies, Tohoku University, Sendai, Miyagi, Japan. FAU - Nakayasu, Yuta AU - Nakayasu Y AUID- ORCID: 0000-0003-2744-099X AD - Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Sendai, Miyagi, Japan. FAU - Kamitakahara, Masanobu AU - Kamitakahara M AUID- ORCID: 0000-0002-3548-7010 AD - Graduate School of Environmental Studies, Tohoku University, Sendai, Miyagi, Japan. LA - eng PT - Journal Article PT - Review PL - Germany TA - ChemSusChem JT - ChemSusChem JID - 101319536 SB - IM OTO - NOTNLM OT - CO2 reduction reaction OT - biocatalysis OT - electrochemistry OT - energy recovery OT - hydrogen evolution OT - nitrogen fixation OT - photophysics EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:40 PHST- 2026/09/03 00:00 [revised] PHST- 2026/03/26 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:40 [entrez] AID - e71162 [pii] AID - 10.1002/cssc.71162 [doi] PST - ppublish SO - ChemSusChem. 2026 Nov 12;19(21):e71162. doi: 10.1002/cssc.71162. PMID- 42954167 OWN - NLM STAT- Publisher LR - 20261029 IS - 1533-2659 (Electronic) IS - 1533-2640 (Linking) DP - 2026 Oct 29 TI - Exploring relapse experiences among polysubstance users: a qualitative study from Southern Iran. PG - 1-26 LID - 10.1080/15332640.2026.2749560 [doi] AB - Relapse is a major challenge in recovering from polysubstance use. Despite the prevalence of relapse, few studies have addressed this issue. The present study was conducted to describe relapses from the perspective of the individuals with polysubstance use disorder. The present study is a qualitative, descriptive work. The participants consisted of the individuals with polysubstance use disorder who were selected by purposeful sampling from educational clinics in southern Iran from October 2021 to November 2022 (n = 25). Data were collected via in depth, semi structured, individual interviews. The data were analyzed via the conventional content analysis method. Four main themes emerged from the analysis: (1) Ambivalence, reflecting conflicting desires to continue or abstain from substance use; (2) Challenges in remaining abstinent, highlighting difficulties in coping with stress and life pressures; (3) Cognitive distortions of users, including illusions of control and rationalizations for substance use; and (4) Lack of comprehensive support, emphasizing insufficient family, social, and healthcare system support. Users' limited coping skills, pursuit of instant pleasure, untreated psychological disorders, identity instability, and inadequate access to health professionals increase relapse risk. Practical interventions, including psychoeducation workshops, structured relapse-prevention programs, and culturally sensitive counseling, can empower users and strengthen the knowledge and skills of healthcare providers. Policymakers should also consider integrating family and community support systems to reduce relapse rates. FAU - Mokhtarpoor, Hassan AU - Mokhtarpoor H AUID- ORCID: 0000-0003-1669-2729 AD - Shiraz University, Shiraz, Iran. FAU - Naderi, Zeinab AU - Naderi Z AUID- ORCID: 0000-0002-7251-943X AD - Department of Nursing, Sirjan School of Medical Sciences, Sirjan, Iran. FAU - Amirinia, Maryam AU - Amirinia M AUID- ORCID: 0000-0003-3846-7258 AD - M.S. in Clinical Psychology, Shiraz University, Shiraz, Iran. FAU - Tehranineshat, Banafsheh AU - Tehranineshat B AUID- ORCID: 0000-0002-2066-5689 AD - Department of Nursing, Faculty of Nursing and Midwifery, Hormozgan University of Medical Sciences, Bandar Abbas, Iran. AD - Community based Psychiatric Care Research Center, Shiraz University of Medical Sciences, Shiraz, Iran. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - J Ethn Subst Abuse JT - Journal of ethnicity in substance abuse JID - 101083217 SB - IM OTO - NOTNLM OT - Substance use OT - polydrug dependence OT - relapse OT - substance use disorder OT - users EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:38 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:38 [entrez] AID - 10.1080/15332640.2026.2749560 [doi] PST - aheadofprint SO - J Ethn Subst Abuse. 2026 Oct 29:1-26. doi: 10.1080/15332640.2026.2749560. PMID- 42954158 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1929-0748 (Electronic) IS - 1929-0748 (Linking) VI - 15 DP - 2026 Oct 28 TI - Multiparametric Magnetic Resonance Imaging in a Prospective Cohort of Living Kidney Donors, Recipients, and Healthy Controls and Correlations With Markers of Renal Function, Fibrosis, and Aging (MPRENAL): Protocol for a Longitudinal Observational Study. PG - e97152 LID - 10.2196/97152 [doi] AB - BACKGROUND: Renal fibrosis is the final common pathway of chronic kidney disease progression and a critical histological predictor of renal function decline and allograft failure. Routine clinical monitoring with estimated glomerular filtration rate (eGFR) and albuminuria is insensitive to fibrosis development. Renal biopsy is invasive and subject to sampling bias, which limits repeated assessment, thus necessitating noninvasive imaging biomarkers. Multiparametric magnetic resonance imaging (mpMRI) characterizes renal structure and function noninvasively. Individual MRI measures have been associated with fibrosis and with declining renal function. However, longitudinal validation against histology and measured glomerular filtration rate (mGFR) remains limited. OBJECTIVE: This prospective longitudinal observational study aims to validate mpMRI against histological fibrosis assessed by kidney biopsy in kidney transplant recipients (KTRs), and compare the time-dependent trajectories of imaging and functional markers across living kidney donors (LKDs), KTRs, and healthy control (HC) cohorts. METHODS: The planned sample includes 32 LKDs, 32 KTRs, and 32 HCs. Up to 40 KTRs may be recruited to account for attrition. Baseline assessments are performed before transplantation for LKDs and KTRs, and at enrollment for HCs, with follow-up assessments at 3, 12, and 24 months. mpMRI is performed at 3 tesla (3 T). The MRI protocol includes structural longitudinal relaxation time (T1)-weighted and T2-weighted imaging, T1 mapping, T2 mapping, transverse relaxation time (T2*) mapping, diffusion-weighted imaging, pseudocontinuous arterial spin labeling, non-contrast enhanced angiography, and phase-contrast measurement of renal artery flow. Glomerular filtration rate will be measured by [(99m)Tc]Tc-diethylenetriaminepentaacetic acid (DTPA) clearance. A baseline allograft biopsy is taken during transplantation surgery, with protocol biopsies at 3, 12, and 24 months. Fibrosis is quantified using stereology. The primary outcome is the longitudinal association between the corticomedullary T1 difference and histologically determined renal fibrosis in KTRs across all 4 biopsy time points. Secondary analyses include longitudinal divergence of MRI and functional markers across cohorts, diagnostic performance for fibrosis, and predictive value for mGFR decline in KTRs and LKDs. Linear mixed models will be used to study longitudinal associations. Three additional MRI measures will be tested in a fixed sequence to control multiplicity. Receiver operating characteristic curve analysis will assess diagnostic performance. RESULTS: The study was funded in October 2023. Recruitment for the multiparametric renal magnetic resonance imaging (MPRENAL) study commenced on October 29, 2024. As of March 2026, 42 participants were enrolled, and data collection is ongoing. Recruitment is anticipated to close in February 2027, with final study visits by March 2029. The primary results are expected to be published in 2030. CONCLUSIONS: Successful completion of this study is expected to provide robust histological validation of mpMRI and establish its utility as a noninvasive tool for monitoring renal health. CI - (c) Patrick Schjelderup, Steffen Ringgaard, Jens Brondum Frokjaer, Jens Dam Jensen, Soren Krag, Jens Randel Nyengaard, Christoffer Laustsen, Niels Henrik Buus, Lars Jodal, Karin Skov, My Hanna Sofia Svensson. Originally published in JMIR Research Protocols (https://www.researchprotocols.org). FAU - Schjelderup, Patrick AU - Schjelderup P AUID- ORCID: 0009-0000-8549-4940 AD - Department of Nephrology, Aalborg University Hospital, Molleparkvej 4, Aalborg, Denmark, +45 97663700. FAU - Ringgaard, Steffen AU - Ringgaard S AUID- ORCID: 0000-0001-5353-7379 AD - The MR Research Centre, Department of Clinical Medicine, Aarhus University, Aarhus, Denmark. FAU - Frokjaer, Jens Brondum AU - Frokjaer JB AUID- ORCID: 0000-0001-8722-0070 AD - Department of Radiology, Aalborg University Hospital, Aalborg, Denmark. AD - Department of Clinical Medicine, Aalborg University, Aalborg, Denmark. FAU - Jensen, Jens Dam AU - Jensen JD AUID- ORCID: 0009-0003-8148-3904 AD - Department of Internal Medicine, Aalborg University Hospital, Hobro, Denmark. FAU - Krag, Soren AU - Krag S AUID- ORCID: 0000-0003-1975-2766 AD - Department of Pathology, Aarhus University Hospital, Aarhus, Denmark. FAU - Nyengaard, Jens Randel AU - Nyengaard JR AUID- ORCID: 0000-0002-8084-4646 AD - Department of Pathology, Aarhus University Hospital, Aarhus, Denmark. AD - Core Center for Molecular Morphology, Department of Clinical Medicine, Aarhus University, Aarhus, Denmark. FAU - Laustsen, Christoffer AU - Laustsen C AUID- ORCID: 0000-0002-0317-2911 AD - The MR Research Centre, Department of Clinical Medicine, Aarhus University, Aarhus, Denmark. FAU - Buus, Niels Henrik AU - Buus NH AUID- ORCID: 0000-0001-7972-8181 AD - Department of Renal Medicine, Aarhus University Hospital, Aarhus, Denmark. FAU - Jodal, Lars AU - Jodal L AUID- ORCID: 0000-0002-6811-0172 AD - Department of Nuclear Medicine, Aalborg University Hospital, Aalborg, Denmark. FAU - Skov, Karin AU - Skov K AUID- ORCID: 0000-0003-1559-4836 AD - Department of Renal Medicine, Aarhus University Hospital, Aarhus, Denmark. FAU - Svensson, My Hanna Sofia AU - Svensson MHS AUID- ORCID: 0009-0008-1255-2722 AD - Department of Nephrology, Aalborg University Hospital, Molleparkvej 4, Aalborg, Denmark, +45 97663700. LA - eng PT - Journal Article PT - Observational Study DEP - 20261028 PL - Canada TA - JMIR Res Protoc JT - JMIR research protocols JID - 101599504 RN - 0 (Biomarkers) SB - IM MH - Humans MH - Prospective Studies MH - *Living Donors/statistics & numerical data MH - Longitudinal Studies MH - Fibrosis MH - *Kidney Transplantation/methods/adverse effects MH - Biomarkers/analysis MH - *Multiparametric Magnetic Resonance Imaging/methods/statistics & numerical data/standards MH - Male MH - Female MH - *Kidney/pathology/diagnostic imaging/physiopathology MH - Glomerular Filtration Rate MH - Kidney Function Tests/methods MH - Middle Aged MH - Adult MH - *Aging/physiology MH - Transplant Recipients/statistics & numerical data OTO - NOTNLM OT - biomarker OT - chronic kidney disease OT - fibrosis OT - glomerular filtration rate OT - kidney transplantation OT - living donors OT - multiparametric magnetic resonance imaging OT - protocol EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:37 PHST- 2026/04/04 00:00 [received] PHST- 2026/08/27 00:00 [revised] PHST- 2026/08/31 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:37 [entrez] AID - v15i1e97152 [pii] AID - 10.2196/97152 [doi] PST - epublish SO - JMIR Res Protoc. 2026 Oct 28;15:e97152. doi: 10.2196/97152. PMID- 42954155 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2562-0959 (Electronic) IS - 2562-0959 (Linking) VI - 9 DP - 2026 Oct 27 TI - Deep Learning-Based Classification of Melanoma Using Dermoscopic Images: Comparative Evaluation of Convolutional Neural Network and DenseNet Models. PG - e104086 LID - 10.2196/104086 [doi] AB - BACKGROUND: For melanoma, an aggressive form of skin cancer, early and accurate detection is critical to improving clinical outcomes. Deep learning-based image analysis has emerged as a promising approach for computer-assisted evaluation of dermoscopic lesions. However, model performance is often affected by limited dataset size, class imbalance, overfitting, and differences in network architecture and optimization strategies. OBJECTIVES: This study was aimed at developing and comparatively evaluating convolutional neural network (CNN) and DenseNet-based deep learning models for automated classification of dermoscopic skin lesion images. The effects of data augmentation, network architecture, optimizer selection, and training duration on classification performance were examined. METHODS: Publicly available dermoscopic skin lesion images obtained from Kaggle were evaluated using a conventional CNN, DenseNet169, and DenseNet201. The original dataset contained 2357 images representing multiple benign and malignant skin lesion categories. Images were resized to 250x250 pixels, labeled, and randomly partitioned into training and testing sets at an 80:20 ratio. To address class imbalance, image augmentation using transformations, including +/-10 degrees shear, was performed, increasing the dataset size to 6857 images, with approximately 500 additional samples generated per class. Models were trained using adaptive moment estimation (Adam) and root mean square propagation (RMSprop) optimizers, and evaluated after 20, 60, and 100 epochs. Performance was assessed using accuracy, precision, recall (sensitivity), specificity, F(1)-score, confusion matrices, and training and validation loss. RESULTS: Data augmentation substantially improved model performance and reduced evidence of overfitting compared with the original dataset. On the augmented dataset, DenseNet169 and DenseNet201 achieved accuracy and F(1)-scores exceeding 94% after 100 epochs, consistently outperforming the conventional CNN. DenseNet169 achieved up to 95% validation accuracy, with only minor performance differences between the Adam and RMSprop optimizers. The CNN showed greater susceptibility to overfitting on the smaller dataset; its F(1)-score, calculated over the complete dataset, increased from 31% after 20 epochs to 59% after 60 epochs and 87% after 100 epochs before augmentation. Across the comparative experiments, the DenseNet architectures demonstrated more stable validation performance and better generalization following augmentation. CONCLUSIONS: DenseNet169 and DenseNet201 demonstrated stronger and more stable classification performance than the conventional CNN for dermoscopic skin lesion classification, particularly after augmentation of the training dataset. Increasing the dataset size and improving class balance led to reduced overfitting and improved model performance. These findings support further investigation of DenseNet-based approaches as potential clinical decision support tools for melanoma and skin lesion assessment. However, prospective evaluation using independent, multicenter datasets and diverse patient populations is required before clinical implementation. CI - (c) Harshal Sanghvi, Argyrios Agritis, Isabella Alvarez, Mitsu Patel, Shuwen Wang, Riki Patel, Raphael Banoub, Timonthy Makkar. Originally published in JMIR Dermatology (http://derma.jmir.org). FAU - Sanghvi, Harshal AU - Sanghvi H AUID- ORCID: 0000-0001-6952-0504 AD - Florida Atlantic University, 777 Glades Road, Boca Raton, FL, 33431, United States, 1 5615634452. FAU - Agritis, Argyrios AU - Agritis A AUID- ORCID: 0009-0008-0092-2570 AD - Department of Technology and Clinical Trials, Advanced Research LLC, Pompano Beach, FL, United States. FAU - Alvarez, Isabella AU - Alvarez I AUID- ORCID: 0009-0004-3049-2082 AD - Department of Technology and Clinical Trials, Advanced Research LLC, Pompano Beach, FL, United States. FAU - Patel, Mitsu AU - Patel M AUID- ORCID: 0009-0001-7191-8375 AD - Department of Technology and Clinical Trials, Advanced Research LLC, Pompano Beach, FL, United States. FAU - Wang, Shuwen AU - Wang S AUID- ORCID: 0000-0003-1899-9628 AD - Clemson University, Clemson, SC, United States. FAU - Patel, Riki AU - Patel R AUID- ORCID: 0000-0003-2519-0958 AD - Department of Technology and Clinical Trials, Advanced Research LLC, Pompano Beach, FL, United States. FAU - Banoub, Raphael AU - Banoub R AUID- ORCID: 0000-0003-0698-156X AD - Mayo Clinic in Florida, Jacksonville, FL, United States. FAU - Makkar, Timonthy AU - Makkar T AUID- ORCID: 0009-0000-5870-827X AD - Department of Dermatology, Rutgers New Jersey Medical School, Brunswick, NJ, United States. LA - eng PT - Comparative Study PT - Journal Article DEP - 20261027 PL - Canada TA - JMIR Dermatol JT - JMIR dermatology JID - 101770607 SB - IM MH - *Dermoscopy/methods MH - Humans MH - *Melanoma/classification/diagnostic imaging/pathology/diagnosis MH - Convolutional Neural Networks MH - *Deep Learning MH - *Skin Neoplasms/classification/diagnostic imaging/pathology/diagnosis MH - Image Processing, Computer-Assisted/methods MH - Classification Algorithms OTO - NOTNLM OT - data augmentation OT - deep learning OT - dermoscopy OT - medical image analysis OT - melanoma detection EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:37 PHST- 2026/06/08 00:00 [received] PHST- 2026/09/02 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:37 [entrez] AID - v9i1e104086 [pii] AID - 10.2196/104086 [doi] PST - epublish SO - JMIR Dermatol. 2026 Oct 27;9:e104086. doi: 10.2196/104086. PMID- 42954151 OWN - NLM STAT- Publisher LR - 20261029 IS - 1466-187X (Electronic) IS - 0142-159X (Linking) DP - 2026 Oct 29 TI - Resisting the merger? Cross-cohort shifts in stress, emotional intelligence, and motivation among medical students (2014-2024). PG - 1-13 LID - 10.1080/0142159X.2026.2746561 [doi] AB - INTRODUCTION: Most studies of medical students' professional identity formation, motivation, and well-being rely on cross-sectional or short-term longitudinal designs, leaving open whether the psychological profile of medical students has shifted across successive cohorts. Empirical, repeated-measures comparisons across multiple cohorts in a single educational context remain rare. Without them, educators cannot tell whether evidence from earlier cohorts still describes the students they teach today. METHODS: A repeated cross-cohort comparison was conducted at a single Polish medical school across six cohorts of second-year students surveyed in 2014, 2015, 2016, 2017, 2018, and 2024 (N = 1595). Academic stress, emotional intelligence (EI; SSEIT), motivation for choosing medical studies, coping strategies, and social support were assessed with the same instruments at each measurement. Cohort differences were examined using Kendall's tau, Kruskal-Wallis tests with Dunn's post hoc comparisons, chi-squared tests, and multiple correspondence analysis (MCA). RESULTS: Self-reported academic stress was lower in the 2018 and 2024 cohorts than in 2014-2017 (Kendall's tau = -0.11, p < 0.0001). Total and interpersonal EI were higher in the 2024 cohort than in the 2015-2018 cohorts (all post hoc p < 0.001); intrapersonal EI remained stable. Financial motivation as a secondary choice rose from 8% to 21%, and 'opportunity to work abroad' disappeared (0% vs. 4.5-8.7%). MCA positioned the 2024 cohort apart from the closely grouped 2014-2018 cohorts. Coping strategies and social support remained largely stable. DISCUSSION: Across 11 years, students at this institution more often selected financial motives when choosing medicine, reported higher interpersonal EI and lower academic stress, with the most recent (post-pandemic) cohort standing apart from earlier cohorts. The design cannot disentangle cohort from period effects, and findings are context-specific. These analyses are exploratory, and any interpretive hypothesis they generate is offered for future testing rather than as an established finding. FAU - Walkiewicz, Maciej AU - Walkiewicz M AUID- ORCID: 0000-0002-5674-5875 AD - Department of Psychology, Medical University of Gdansk, Gdansk, Poland. FAU - Guziak, Mateusz AU - Guziak M AUID- ORCID: 0000-0002-5720-3410 AD - Department of Psychology, Medical University of Gdansk, Gdansk, Poland. FAU - Koelmer, Anna AU - Koelmer A AUID- ORCID: 0000-0003-1197-9405 AD - Centre of Biostatistics and Bioinformatics Analysis, Medical University of Gdansk, Gdansk, Poland. FAU - Nowicka-Sauer, Katarzyna AU - Nowicka-Sauer K AUID- ORCID: 0000-0002-7510-5478 AD - Department of Family Medicine, Medical University of Gdansk, Gdansk, Poland. FAU - Zimmermann, Agnieszka AU - Zimmermann A AUID- ORCID: 0000-0002-8757-5311 AD - Department of Medical and Pharmacy Law, Medical University of Gdansk, Gdansk, Poland. FAU - Blazek, Magdalena AU - Blazek M AUID- ORCID: 0000-0003-3675-1474 AD - Department of Psychology, Medical University of Gdansk, Gdansk, Poland. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Med Teach JT - Medical teacher JID - 7909593 SB - IM OTO - NOTNLM OT - Professional identity formation OT - academic stress OT - emotional intelligence OT - medical students OT - motivation EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:37 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:37 [entrez] AID - 10.1080/0142159X.2026.2746561 [doi] PST - aheadofprint SO - Med Teach. 2026 Oct 29:1-13. doi: 10.1080/0142159X.2026.2746561. PMID- 42954150 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1929-0748 (Electronic) IS - 1929-0748 (Linking) VI - 15 DP - 2026 Oct 27 TI - Effects of Oral Resveratrol Supplementation on Inflammatory and Cardiometabolic Outcomes in Adults: Protocol for a Systematic Review and Meta-Analysis. PG - e101301 LID - 10.2196/101301 [doi] AB - BACKGROUND: Resveratrol is a naturally occurring polyphenol found primarily in grapes, berries, peanuts, and red wine. Experimental evidence suggests that resveratrol modulates multiple biological pathways involved in oxidative stress, inflammation, insulin signaling, endothelial function, and metabolic homeostasis. However, findings from randomized controlled trials remain inconsistent, and previous evidence syntheses have reported substantial clinical and methodological heterogeneity. OBJECTIVE: This is a protocol for a systematic review and meta-analysis that will provide an updated synthesis of randomized controlled trials evaluating the effects of oral resveratrol supplementation on inflammatory biomarkers and cardiometabolic outcomes in adults while exploring potential methodological and biological sources of heterogeneity. METHODS: This protocol was developed according to the PRISMA-P (Preferred Reporting Items for Systematic Reviews and Meta-Analyses Protocols) statement and prospectively registered in PROSPERO. Randomized controlled trials comparing oral resveratrol supplementation with placebo, other nutritional supplements, or no intervention in adults will be eligible. Literature searches will be conducted in the PubMed, Cochrane CENTRAL, Scopus, Web of Science, and SciELO databases. Two reviewers will independently perform study selection, data extraction, and risk-of-bias assessment using the Cochrane risk of bias 2 tool. The certainty of evidence will be assessed using the GRADE (Grading of Recommendations Assessment, Development and Evaluation) approach. RESULTS: During peer review, the search strategy was refined before initiation of the formal review. As of August 2026, the formal systematic review had not yet commenced. The literature search and study selection are scheduled to begin on September 1, 2026, and the systematic review is expected to be completed by April 2027, with dissemination of the findings anticipated thereafter. CONCLUSIONS: This systematic review and meta-analysis protocol is expected to provide an updated and transparent synthesis of the available randomized evidence regarding the effects of oral resveratrol supplementation on inflammatory biomarkers and cardiometabolic outcomes in adults. CI - (c) Andrea Herrera-Santelices, Josefa Camila Nunez Rivas, Francisca P Rubio-Molina, Ileana Gonzalez Bonet. Originally published in JMIR Research Protocols (https://www.researchprotocols.org). FAU - Herrera-Santelices, Andrea AU - Herrera-Santelices A AUID- ORCID: 0000-0002-9691-1874 AD - Departamento de Ciencias Preclinicas, Facultad de Medicina, Universidad Catolica del Maule, Avda San Miguel 3605, Talca, Maule, Chile, 56 712968000. AD - Servicio de Medicina Fisica y Rehabilitacion, Hospital San Juan de Dios de Curico, Servicio de Salud del Maule, Curico, Maule, Chile. FAU - Nunez Rivas, Josefa Camila AU - Nunez Rivas JC AUID- ORCID: 0009-0007-4094-8495 AD - Carrera de Medicina, Facultad de Medicina, Universidad Catolica del Maule, Talca, Maule, Chile. FAU - Rubio-Molina, Francisca P AU - Rubio-Molina FP AUID- ORCID: 0000-0002-9070-4655 AD - Sistema de Bibliotecas, Universidad Catolica del Maule, Talca, Maule, Chile. FAU - Gonzalez Bonet, Ileana AU - Gonzalez Bonet I AUID- ORCID: 0000-0003-3331-9299 AD - Departamento de Ciencias Preclinicas, Facultad de Medicina, Universidad Catolica del Maule, Avda San Miguel 3605, Talca, Maule, Chile, 56 712968000. LA - eng PT - Journal Article DEP - 20261027 PL - Canada TA - JMIR Res Protoc JT - JMIR research protocols JID - 101599504 RN - Q369O8926L (Resveratrol) RN - 0 (Biomarkers) SB - IM MH - *Resveratrol/pharmacology/administration & dosage/therapeutic use MH - Humans MH - Meta-Analysis as Topic MH - Systematic Reviews as Topic MH - *Inflammation/drug therapy MH - *Dietary Supplements MH - Administration, Oral MH - Adult MH - Biomarkers OTO - NOTNLM OT - cardiometabolic outcomes OT - inflammatory biomarkers OT - meta-analysis OT - randomized controlled trial OT - resveratrol OT - systematic review EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:37 PHST- 2026/05/13 00:00 [received] PHST- 2026/08/24 00:00 [revised] PHST- 2026/08/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:37 [entrez] AID - v15i1e101301 [pii] AID - 10.2196/101301 [doi] PST - epublish SO - JMIR Res Protoc. 2026 Oct 27;15:e101301. doi: 10.2196/101301. PMID- 42954144 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1724-6008 (Electronic) IS - 0393-6155 (Linking) VI - 41 DP - 2026 Jan-Dec TI - FPSA, a potential risk factor for thyroid nodules by gender: A cohort study in China. PG - 3936155261503538 LID - 10.1177/03936155261503538 [doi] AB - BackgroundThe global prevalence of thyroid nodules is rising, prompting heightened clinical concern given their potential for malignant transformation. Although tumor markers are routinely assessed to aid in malignancy evaluation, their potential to predict thyroid nodule development and their value for early risk stratification remain insufficiently established.MethodsIn this prospective cohort study, 462 adults underwent three standardized physical examinations after enrollment. Comprehensive baseline data, including demographics, anthropometrics, vital signs, laboratory parameters, and ultrasound-confirmed thyroid nodule status have been collected. variance inflation factor and Least Absolute Shrinkage and Selection Operator were used in variable selection while logistic regression was used in statistical analysis.ResultsA total of 302 participants were included in the final analytical cohort. In this cohort, female sex, age, BMI, and DBP were significantly associated with the occurrence of thyroid nodules. When further analyzed using a discrete-time hazard model, age, SBP, and FPSA were significantly associated with nodule occurrence at the second and third examination. In males, logistic regression identified age, BMI, and FPSA as significant risk factors, and the discrete-time hazard model confirmed age, FPSA as independent predictors. In females, however, logistic regression showed that age was a risk factor while DBP was a protective factor, findings that were replicated in the discrete-time hazard model.ConclusionFemale sex, age, BMI, DBP, and FPSA show distinct associations with thyroid nodule development: FPSA is a male-specific risk marker, while DBP is protective in females. These findings point to sex-specific biological pathways and warrant mechanistic study and validation in diverse populations. FAU - Yang, Wentao AU - Yang W AD - Department of Endocrinology, Children's Hospital of Nanjing Medical University, Nanjing, China. RINGGOLD: 159388 FAU - Chu, Shanshan AU - Chu S AD - Department of Endocrinology, Children's Hospital of Nanjing Medical University, Nanjing, China. RINGGOLD: 159388 FAU - Wu, Wenqian AU - Wu W AD - Department of Emergency, Pediatric Intensive Care Unit, Children's Hospital of Nanjing Medical University, Nanjing, China. RINGGOLD: 159388 FAU - Shi, Tingting AU - Shi T AD - Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, China. RINGGOLD: 66478 FAU - Wang, Xu AU - Wang X AUID- ORCID: 0000-0002-1861-5922 AD - Pediatric Clinical Medical Research Center, Children's Hospital of Nanjing Medical University, Nanjing, China. RINGGOLD: 159388 FAU - Xu, Penghong AU - Xu P AD - Department of Emergency, Pediatric Intensive Care Unit, Children's Hospital of Nanjing Medical University, Nanjing, China. RINGGOLD: 159388 LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Int J Biol Markers JT - The International journal of biological markers JID - 8712411 RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - *Thyroid Nodule/epidemiology/pathology/genetics/metabolism MH - Female MH - China/epidemiology MH - Risk Factors MH - Middle Aged MH - Male MH - Adult MH - Prospective Studies MH - Sex Factors MH - *Biomarkers, Tumor MH - Cohort Studies OTO - NOTNLM OT - BMI OT - FPSA OT - cohort study OT - thyroid nodules OT - tumor markers COIS- Declaration of conflicting interestsThe authors declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest or non-financial interest in the subject matter or materials discussed in this manuscript. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:36 PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:36 [entrez] AID - 10.1177/03936155261503538 [doi] PST - ppublish SO - Int J Biol Markers. 2026 Jan-Dec;41:3936155261503538. doi: 10.1177/03936155261503538. Epub 2026 Oct 29. PMID- 42954138 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2173-9110 (Electronic) IS - 1135-5727 (Linking) VI - 100 DP - 2026 Oct 29 TI - [Nervous System Anomalies in infants less than one year of Valencian Region (Spain). A descriptive epidemiological study (2007-2022)]. LID - e202610085 [pii] AB - OBJECTIVE: Nervous system congenital anomalies (NSCA) are an important health problem and represent a high perinatal and infant mortality. The aim of this paper was to determine and describe the trend and distribution of the NSCA in the Valencian Region, in children under one year of age, during the period 2007-2022. METHODS: A cross-sectional study was performed. Live births, stillbirths and termination of pregnancy for fetal anomaly with NSCA were selected from the congenital anomalies population-based Registry of Valencian Region between 2007-2022. The study variables were described, the prevalence according to EUROCAT methodology per 10,000 births (included live and still birth) and the survival rate in live births was estimated using the Kaplan-Meier method. Statistical analyses were performed using Microsoft Excel and IBM SPSS Statistics 23. RESULTS: 1,862 cases were identified, the overall prevalence was 26.1/10,000 births. The most common type of end-pregnancy was live births (54.6%) and Hydrocephalus was the most frequent congenital malformation subgroup of NSCA (20.7%). In live births, the survival rate was 94.9% and statistically significant differences were identified according to NSCA. Regarding the pregnant women, mean age was 31.9 years and the majority birth country was Spain (69.1%). The highest prevalence was in the group under twenty years old (38.4/10,000 births) and in pregnant women resident in Castellon (34.1/10,000 births). CONCLUSIONS: The prevalence of cases with NSCA is similar than European network registries of congenital anomalies. Identifying higher prevalence of cases among the youngest pregnant women or residents in Castellon, it could be connected to the higher presence in that province of pregnant women from certain countries. FAU - Gimeno-Martos, Silvia AU - Gimeno-Martos S AD - Centro de Salud Publica de Torrente. Generalitat Valenciana. Valencia. Espana. FAU - Garcia-Villodre, Laura AU - Garcia-Villodre L AD - Area de Investigacion Enfermedades Raras. Fundacion para el Fomento de la Investigacion Sanitaria y Biomedica de la Comunitat Valenciana (Fundacio FISABIO). Valencia. Espana. FAU - Paramo-Rodriguez, Lucia AU - Paramo-Rodriguez L AD - Area de Investigacion Enfermedades Raras. Fundacion para el Fomento de la Investigacion Sanitaria y Biomedica de la Comunitat Valenciana (Fundacio FISABIO). Valencia. Espana. AD - Unidad Mixta de Investigacion en Enfermedades Raras. Fundacion para el Fomento de la Investigacion Sanitaria y Biomedica de la Comunitat Valenciana-Universitat de Valencia (FISABIO-UVEG). Valencia. Espana. FAU - Arribas-Diaz, Berta AU - Arribas-Diaz B AD - Area de Investigacion Enfermedades Raras. Fundacion para el Fomento de la Investigacion Sanitaria y Biomedica de la Comunitat Valenciana (Fundacio FISABIO). Valencia. Espana. FAU - Barrachina-Bonet, Laia AU - Barrachina-Bonet L AD - Area de Investigacion Enfermedades Raras. Fundacion para el Fomento de la Investigacion Sanitaria y Biomedica de la Comunitat Valenciana (Fundacio FISABIO). Valencia. Espana. AD - Unidad Mixta de Investigacion en Enfermedades Raras. Fundacion para el Fomento de la Investigacion Sanitaria y Biomedica de la Comunitat Valenciana-Universitat de Valencia (FISABIO-UVEG). Valencia. Espana. FAU - Martos, Carmen AU - Martos C AD - Area de Investigacion Enfermedades Raras. Fundacion para el Fomento de la Investigacion Sanitaria y Biomedica de la Comunitat Valenciana (Fundacio FISABIO). Valencia. Espana. AD - Unidad Mixta de Investigacion en Enfermedades Raras. Fundacion para el Fomento de la Investigacion Sanitaria y Biomedica de la Comunitat Valenciana-Universitat de Valencia (FISABIO-UVEG). Valencia. Espana. FAU - Cavero-Carbonell, Clara AU - Cavero-Carbonell C AD - Area de Investigacion Enfermedades Raras. Fundacion para el Fomento de la Investigacion Sanitaria y Biomedica de la Comunitat Valenciana (Fundacio FISABIO). Valencia. Espana. AD - Unidad Mixta de Investigacion en Enfermedades Raras. Fundacion para el Fomento de la Investigacion Sanitaria y Biomedica de la Comunitat Valenciana-Universitat de Valencia (FISABIO-UVEG). Valencia. Espana. LA - spa PT - English Abstract PT - Journal Article TT - Anomalias congenitas del sistema nervioso en menores de un ano en la Comunitat Valenciana. Un estudio epidemiologico descriptivo (2007-2022). DEP - 20261029 PL - Spain TA - Rev Esp Salud Publica JT - Revista espanola de salud publica JID - 9600212 SB - IM MH - Humans MH - Spain/epidemiology MH - Cross-Sectional Studies MH - Female MH - Prevalence MH - Infant, Newborn MH - Infant MH - *Nervous System Malformations/epidemiology MH - Pregnancy MH - Male MH - Adult OTO - NOTNLM OT - Congenital anomalies OT - Epidemiology OT - Health information systems OT - Nervous system OT - Prevalence OT - Spain EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:36 PHST- 2025/07/28 00:00 [received] PHST- 2026/07/02 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:36 [entrez] AID - e202610085 [pii] PST - epublish SO - Rev Esp Salud Publica. 2026 Oct 29;100:e202610085. PMID- 42954134 OWN - NLM STAT- Publisher LR - 20261029 IS - 2055-6764 (Electronic) IS - 2055-6756 (Linking) DP - 2026 Oct 29 TI - Design criteria for HTL-blended emissive layers in red quantum dot light-emitting diodes. LID - 10.1039/d6nh00336b [doi] AB - The use of hole transport layer (HTL)-blended emissive layers (EMLs) has been explored as an effective strategy for improving charge balance in quantum dot light-emitting diodes (QD-LEDs). However, their potential has not been fully realized because clear criteria for selecting suitable HTL materials are still lacking. Here, using a red-emitting CdZnSe core/shell QD/ZnMgO system, we identify two key criteria for HTLs in blended EMLs: efficient hole transport and tolerance to electron leakage at additional local HTL/electron transport layer (ETL) contacts that may form within the blended EML. Using three representative polymeric HTLs-PVK, TFB, and PF8Cz-we investigated how HTL-dependent charge transport and leakage susceptibility affect recombination behavior and device performance. For all three HTLs, hole-only devices (HODs) with blended EMLs exhibited higher current densities, indicating that HTL blending facilitated hole transport by providing additional hole-injection pathways throughout the EML. In contrast, voltage-evolution measurements of HTL/ZnMgO junction devices under constant-current operation revealed a strong dependence on the HTL material. The TFB-based junction device exhibited substantial voltage drift, while the corresponding TFB-blended QD-LED showed a distinct TFB-related shoulder emission, consistent with parasitic recombination. By contrast, the PVK- and PF8Cz-blended QD-LEDs exhibited no detectable HTL-related parasitic emission. Among the examined materials, the PF8Cz-blended device enabled lower-voltage operation, a broader near-unity S regime, and the largest relative enhancement in external quantum efficiency. These results establish hole-transport capability and tolerance to electron leakage at potential local HTL/ETL contacts as practical criteria for selecting HTLs for high-performance red QD-LEDs with blended EMLs. FAU - Lee, Kyoungeun AU - Lee K AD - Department of Electrical Engineering, Pusan National University, Busan, 46241, Republic of Korea. jkroh@pusan.ac.kr. FAU - Lee, Kyunghoon AU - Lee K AUID- ORCID: 0000-0002-5628-2057 AD - Department of Energy Science & Engineering, Daegu Gyeongbuk Institute of Science & Technology (DGIST), Daegu, 42988, Republic of Korea. FAU - Bae, Yeyun AU - Bae Y AD - Department of Electrical Engineering, Pusan National University, Busan, 46241, Republic of Korea. jkroh@pusan.ac.kr. FAU - Lee, Jaeyeop AU - Lee J AD - Department of Electrical Engineering, Pusan National University, Busan, 46241, Republic of Korea. jkroh@pusan.ac.kr. FAU - Lee, Hyeonjun AU - Lee H AD - Laboratoire de Physique de la Matiere Condensee, Ecole Polytechnique, CNRS, Institut Polytechnique de Paris, Route de Saclay, 91128 Palaiseau Cedex, France. FAU - Lim, Jaehoon AU - Lim J AUID- ORCID: 0000-0003-2623-3550 AD - Department of Energy Science and Center for Artificial Atoms, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do 16419, Republic of Korea. AD - Department of Display Engineering (DDE), Sungkyunkwan University, Suwon, Gyeonggi-do 16419, Republic of Korea. AD - Department of Future Energy Engineering (DFEE), Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do 16419, Republic of Korea. FAU - Yang, Jiwoong AU - Yang J AUID- ORCID: 0000-0002-2346-8197 AD - Department of Energy Science & Engineering, Daegu Gyeongbuk Institute of Science & Technology (DGIST), Daegu, 42988, Republic of Korea. AD - DGIST InnoCORE Initiative, Center for Energy Materials Commercialization through AI Transformation (AX) (E-MatAX), Daegu Gyeongbuk Institute of Science & Technology (DGIST), Daegu 42988, Republic of Korea. AD - Energy Science and Engineering Research Center, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu, Republic of Korea. FAU - Kim, Chansu AU - Kim C AUID- ORCID: 0009-0002-7919-4866 AD - MOPLAT Inc., 306, Seongsu SK V1 Center II, 31, Gwangnaru-ro 8-gil, Seongdong-gu, Seoul 04799, Republic of Korea. FAU - Roh, Jeongkyun AU - Roh J AUID- ORCID: 0000-0002-0674-572X AD - Department of Electrical Engineering, Pusan National University, Busan, 46241, Republic of Korea. jkroh@pusan.ac.kr. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Nanoscale Horiz JT - Nanoscale horizons JID - 101712576 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:36 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:36 [entrez] AID - 10.1039/d6nh00336b [doi] PST - aheadofprint SO - Nanoscale Horiz. 2026 Oct 29. doi: 10.1039/d6nh00336b. PMID- 42954131 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261030 IS - 1440-1827 (Electronic) IS - 1320-5463 (Linking) VI - 76 IP - 10 DP - 2026 Oct TI - TRMT112 Expression Is Associated With Poor Prognosis and Increased Invasive Potential in Oral Squamous Cell Carcinoma. LID - e70197 [pii] LID - 10.1111/pin.70197 [doi] AB - Oral squamous cell carcinoma (OSCC) has a poor prognosis and is frequently diagnosed at advanced stages. Therefore, the identification of clinically useful tumor biomarkers is a critical unmet need. The tRNA methyltransferase subunit 11-2 (TRMT112) forms a complex with methyltransferase-like protein 5 (METTL5) and is involved in RNA methylation; however, limited evidence suggests a role for TRMT112 as a tumor-promoting factor in cancer. Therefore, the biological function of TRMT112 in cancer, including OSCC, remains largely unknown, prompting this study. Analyses of public databases revealed that TRMT112 expression was associated with poor prognosis in head and neck squamous cell carcinoma and was positively correlated with METTL5 expression levels. TRMT112 was also associated with increased sensitivity to osimertinib and lestaurtinib. Furthermore, RNA polymerase II subunit G (POLR2G) and Sjogren's syndrome/scleroderma autoantigen 1 (SSSCA1, also termed ZNRD2) were identified as newly related genes. Immunohistochemical staining revealed that the TRMT112 protein was associated with an invasive growth pattern (p = 0.0234) and poorer prognosis (p = 0.0205). Functional assays indicated that TRMT112 was associated with increased invasive potential and regulation of METTL5 expression in OSCC cells. These findings suggest that TRMT112 may serve as a potential diagnostic and therapeutic target in OSCC. CI - (c) 2026 The Author(s). Pathology International published by Japanese Society of Pathology and John Wiley & Sons Australia, Ltd. FAU - Oku, Yasunobu AU - Oku Y AD - Department of Molecular Oral Pathology and Oncology, Field of Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan. FAU - Shima, Kaori AU - Shima K AUID- ORCID: 0000-0001-7849-7879 AD - Department of Molecular Oral Pathology and Oncology, Field of Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan. FAU - Kajiya, Yuka AU - Kajiya Y AD - Department of Molecular Oral Pathology and Oncology, Field of Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan. FAU - Shimojukkoku, Yudai AU - Shimojukkoku Y AUID- ORCID: 0009-0006-4595-0626 AD - Department of Molecular Oral Pathology and Oncology, Field of Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan. AD - Department of Oral Surgery, Saitama Cancer Center, Ina, Japan. FAU - Kurihara-Shimomura, Miyako AU - Kurihara-Shimomura M AD - Department of Oral and Maxillofacial Surgery, Nara Medical University, Kashihara, Japan. FAU - Sasahira, Tomonori AU - Sasahira T AUID- ORCID: 0000-0003-1757-1275 AD - Department of Molecular Oral Pathology and Oncology, Field of Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan. LA - eng GR - 24K02647/Japan Society for the Promotion of Science/ GR - 25K13152/Japan Society for the Promotion of Science/ GR - 25K13191/Japan Society for the Promotion of Science/ GR - Takahashi Industrial and Economic Research Foundation/ GR - Daiwa Securities Health Foundation/ PT - Journal Article PL - Australia TA - Pathol Int JT - Pathology international JID - 9431380 RN - 0 (Biomarkers, Tumor) RN - EC 2.1.1.- (tRNA Methyltransferases) SB - IM MH - Humans MH - Prognosis MH - *Mouth Neoplasms/pathology/metabolism MH - *Biomarkers, Tumor/metabolism MH - *Carcinoma, Squamous Cell/pathology/metabolism MH - Female MH - *tRNA Methyltransferases/metabolism/genetics MH - Male MH - Neoplasm Invasiveness/pathology MH - *Squamous Cell Carcinoma of Head and Neck/pathology/metabolism MH - Gene Expression Regulation, Neoplastic MH - Middle Aged OTO - NOTNLM OT - METTL5 OT - TRMT112 OT - invasion OT - oral cancer OT - prognosis EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:35 PHST- 2026/09/17 00:00 [revised] PHST- 2026/04/30 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:35 [entrez] AID - e70197 [pii] AID - 10.1111/pin.70197 [doi] PST - ppublish SO - Pathol Int. 2026 Oct;76(10):e70197. doi: 10.1111/pin.70197. PMID- 42954122 OWN - NLM STAT- Publisher LR - 20261029 IS - 1521-6950 (Electronic) IS - 1093-7404 (Linking) DP - 2026 Oct 29 TI - Environmental PM(2.5) exposure associated with lung cancer in nonsmokers: a narrative review of high-risk groups, mechanisms, and implications for screening. PG - 1-20 LID - 10.1080/10937404.2026.2747089 [doi] AB - Lung cancer remains the leading cause of cancer-related death worldwide, and an increasing proportion of cases occur in lifetime nonsmokers, particularly East Asian women. Fine particulate matter (PM(2.5)) in outdoor air pollution is classified as a Group 1 carcinogen and increasingly seen as an important modifiable exposure associated with lung cancer in nonsmokers. This narrative review summarizes epidemiologic and toxicological evidence linking environmental and microenvironmental PM(2.5) exposures to lung cancer in nonsmokers, defined as individuals who have smoked fewer than 100 cigarettes in their lifetime. Global cohort and meta-analytic data, including dose-response findings and studies from Asian populations, are reviewed. Biological pathways linking PM(2.5) to lung carcinogenesis are then examined, including oxidative stress, pulmonary inflammation, and particle-triggered cytokine and growth-factor signaling. Particular emphasis is placed on macrophage-derived interleukin-1beta (IL-1beta) signaling, which promotes epidermal growth factor receptor (EGFR)-mutant lung adenocarcinoma. High-exposure microenvironments such as religious incense burning, residential cooking and solid-fuel use, urban transit systems (subway, bus, motorbike, and professional driving), and selected occupational settings (career and wildland firefighters, construction trades, informal e-waste recycling) are identified. These settings might contribute significantly to daily PM(2.5) exposure in some individuals. Finally, future research priorities, including incorporation of cumulative PM(2.5) exposure into lung cancer risk prediction models and development of cost-effective strategies for lung cancer screening in nonsmokers with high PM(2.5) exposure, are presented. FAU - Wang, Cheng-Yi AU - Wang CY AUID- ORCID: 0000-0001-7426-0743 AD - Division of Pulmonary Medicine, Department of Internal Medicine, Cardinal Tien Hospital and School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan. FAU - Lim, Biing-Suan AU - Lim BS AD - Division of Pulmonary Medicine, Department of Internal Medicine, Cardinal Tien Hospital and School of Medicine, College of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan. FAU - Huang, Yuh-Chin T AU - Huang YT AD - Department of Medicine, Duke University Medical Center, Durham, NC, USA. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - J Toxicol Environ Health B Crit Rev JT - Journal of toxicology and environmental health. Part B, Critical reviews JID - 9802627 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:35 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:35 [entrez] AID - 10.1080/10937404.2026.2747089 [doi] PST - aheadofprint SO - J Toxicol Environ Health B Crit Rev. 2026 Oct 29:1-20. doi: 10.1080/10937404.2026.2747089. PMID- 42954110 OWN - NLM STAT- Publisher LR - 20261029 IS - 1360-046X (Electronic) IS - 0268-8697 (Linking) DP - 2026 Oct 29 TI - Contralateral acute subdural hematoma following moyamoya bypass surgery: a rare complication. PG - 1-6 LID - 10.1080/02688697.2026.2753131 [doi] AB - BACKGROUND: Moyamoya disease (MMD) is a progressive cerebrovascular disorder characterized by compensatory development of collateral vessels in response to internal carotid artery occlusion. Superficial temporal artery to middle cerebral artery (STA-MCA) bypass combined with encephalomyosynangiosis is a standard surgical intervention, hemorrhagic complications following such procedures are rare, especially in pediatric patients. We present a rare case of contralateral acute subdural hematoma (ASDH) occurring immediately after revascularization surgery in a child with MMD. CASE DESCRIPTION: A 7-year-old girl with a history of left cerebral infarction due to MMD underwent left STA-MCA bypass with encephalomyosynangiosis. Although the bypass was technically successful and the patient showed no new neurological deficits postoperatively, routine computed tomography revealed a contralateral ASDH. The hematoma was promptly evacuated. Intraoperative findings included oozing from the dura near the sphenoid ridge, but no active bleeding source was identified. She recovered well without neurological deterioration. Notably, follow-up digital subtraction angiography at five months postoperatively revealed the formation of a spontaneous transdural anastomosis from the right middle meningeal artery, eliminating the need for further bypass surgery on the contralateral side. CONCLUSION: Cerebral atrophy, intraoperative cerebrospinal fluid drainage, and perioperative aspirin use may have contributed to the development of contralateral ASDH. Surgeons should be aware of this possibility. Prompt recognition and evacuation are essential. FAU - Akiyama, Junichi AU - Akiyama J AD - Department of Neurosurgery, The University of Tokyo Hospital, Tokyo, Japan. FAU - Hong, Sukwoo AU - Hong S AD - Department of Neurosurgery, The University of Tokyo Hospital, Tokyo, Japan. FAU - Miyawaki, Satoru AU - Miyawaki S AD - Department of Neurosurgery, The University of Tokyo Hospital, Tokyo, Japan. FAU - Ono, Hideaki AU - Ono H AD - Department of Neurosurgery, The University of Tokyo Hospital, Tokyo, Japan. FAU - Saito, Nobuhito AU - Saito N AD - Department of Neurosurgery, The University of Tokyo Hospital, Tokyo, Japan. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Br J Neurosurg JT - British journal of neurosurgery JID - 8800054 SB - IM OTO - NOTNLM OT - Moyamoya disease OT - acute subdural hematoma OT - contralateral complication OT - pediatric neurosurgery OT - revascularization surgery EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:34 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:34 [entrez] AID - 10.1080/02688697.2026.2753131 [doi] PST - aheadofprint SO - Br J Neurosurg. 2026 Oct 29:1-6. doi: 10.1080/02688697.2026.2753131. PMID- 42954108 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2291-9694 (Electronic) IS - 2291-9694 (Linking) VI - 14 DP - 2026 Oct 23 TI - Explainable Machine Learning for Predicting Major Adverse Renal and Cardiovascular Events in Acute Myocardial Infarction With Chronic Kidney Disease: Algorithm Development and Validation. PG - e96654 LID - 10.2196/96654 [doi] AB - BACKGROUND: Major adverse renal and cardiovascular events (MARCE) have been proposed as a comprehensive end point reflecting both cardiac and renal outcomes in patients with acute myocardial infarction (AMI) and chronic kidney disease (CKD). However, existing prediction tools lack accuracy and generalizability for this high-risk population. OBJECTIVE: This study aimed to develop and externally validate explainable machine learning models and a nomogram for predicting in-hospital MARCE among patients with AMI and CKD. METHODS: We enrolled patients with AMI and CKD who underwent emergency treatment at the First Affiliated Hospital of Wenzhou Medical University as the discovery cohort and those at Wenzhou TCM (traditional Chinese medicine) Hospital of Zhejiang Chinese Medical University as the external validation cohort. Clinical features, including demographic details, comorbidities, laboratory results, echocardiography measurements, procedural details, and medication parameters, were collected for all participants. Important variables were selected using least absolute shrinkage and selection operator regression in the training set. We developed extreme gradient boosting (XGBoost), random forest, decision tree, and logistic regression (LR) models and evaluated their discrimination, calibration, and classification metrics in the hold-out test set and external cohort. RESULTS: In the training set, we identified 6 important variables (Killip class, lactate, estimated glomerular filtration rate, use of diuretics, use of beta-blockers, and use of renin-angiotensin system inhibitors). In the test set, model discrimination was comparable across XGBoost (area under the receiver operating characteristic curve [AUC] 0.882, 95% CI 0.816-0.947), LR (AUC 0.875, 95% CI 0.799-0.950), and random forest (AUC 0.870, 95% CI 0.800-0.940), while decision tree showed lower performance (AUC 0.814, 95% CI 0.720-0.886). Shapley additive explanations (SHAP) values illustrated both global and individual feature contributions in the XGBoost model. A nomogram based on LR achieved an AUC of 0.873 (95% CI 0.714-0.926) in external validation. CONCLUSIONS: Our study identified 6 important predictors and provided a series of prediction models to enhance risk stratification of MARCE for patients with AMI and CKD. These models might aid in stratifying risk and choosing appropriate therapeutic strategies in the management of patients with AMI and CKD. CI - (c) Wang Feng, Ruihao Jiang, Wendi Huang, Chuanpeng Wang, Zhuoquan Wang, Jingwen Chang, Jinguo Cheng, Nianshao Chen, Xi Liu. Originally published in JMIR Medical Informatics (https://medinform.jmir.org). FAU - Feng, Wang AU - Feng W AUID- ORCID: 0009-0008-9987-2365 AD - Wenzhou TCM Hospital of Zhejiang Chinese Medical University, Wenzhou, Zhejiang, China. FAU - Jiang, Ruihao AU - Jiang R AUID- ORCID: 0000-0002-3268-7893 AD - First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China. FAU - Huang, Wendi AU - Huang W AUID- ORCID: 0009-0001-7644-3700 AD - Wenzhou TCM Hospital of Zhejiang Chinese Medical University, Wenzhou, Zhejiang, China. FAU - Wang, Chuanpeng AU - Wang C AUID- ORCID: 0009-0003-0778-9315 AD - Wenzhou TCM Hospital of Zhejiang Chinese Medical University, Wenzhou, Zhejiang, China. FAU - Wang, Zhuoquan AU - Wang Z AUID- ORCID: 0009-0006-9838-5376 AD - Wenzhou TCM Hospital of Zhejiang Chinese Medical University, Wenzhou, Zhejiang, China. FAU - Chang, Jingwen AU - Chang J AUID- ORCID: 0009-0002-4768-1213 AD - Wenzhou TCM Hospital of Zhejiang Chinese Medical University, Wenzhou, Zhejiang, China. FAU - Cheng, Jinguo AU - Cheng J AUID- ORCID: 0000-0003-1616-5822 AD - First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China. FAU - Chen, Nianshao AU - Chen N AUID- ORCID: 0009-0001-9386-6369 AD - Wenzhou TCM Hospital of Zhejiang Chinese Medical University, Wenzhou, Zhejiang, China. FAU - Liu, Xi AU - Liu X AUID- ORCID: 0000-0002-0344-7209 AD - Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University, Xueyuan West Road, Lucheng District, Wenzhou, Zhejiang, 325000, China, 86 18857851776. LA - eng PT - Journal Article PT - Validation Study DEP - 20261023 PL - Canada TA - JMIR Med Inform JT - JMIR medical informatics JID - 101645109 SB - IM MH - Humans MH - *Renal Insufficiency, Chronic/complications/epidemiology MH - *Myocardial Infarction/complications/epidemiology MH - *Machine Learning MH - Female MH - Male MH - Middle Aged MH - Boosting Machine Learning Algorithms MH - Aged MH - Predictive Learning Models MH - Random Forest MH - Prediction Algorithms MH - Classification Algorithms MH - Logistic Models MH - Nomograms MH - Algorithms MH - ROC Curve OTO - NOTNLM OT - acute myocardial infarction OT - chronic kidney disease OT - machine learning OT - major adverse renal and cardiovascular events OT - nomogram EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:34 PHST- 2026/03/30 00:00 [received] PHST- 2026/06/27 00:00 [revised] PHST- 2026/06/29 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:34 [entrez] AID - v14i1e96654 [pii] AID - 10.2196/96654 [doi] PST - epublish SO - JMIR Med Inform. 2026 Oct 23;14:e96654. doi: 10.2196/96654. PMID- 42954094 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1438-8871 (Electronic) IS - 1438-8871 (Linking) VI - 28 DP - 2026 Oct 21 TI - Environmental Impact of Digital Health Care Technologies in Reducing Carbon Footprints: Scoping Review. PG - e88341 LID - 10.2196/88341 [doi] AB - BACKGROUND: Globally, the carbon footprint of the health care sector is significant and growing, and digital health care technologies (DHTs) offer unique opportunities to reduce the carbon footprint of health and care systems. However, their environmental impact has not been adequately investigated. OBJECTIVE: This scoping review aims to explore the environmental impact of DHTs, focusing on reducing their carbon footprint and evaluating methods for assessing that impact. METHODS: This scoping review followed the method outlined by Arksey and O'Malley, adhering to the PRISMA-ScR (Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews) guidelines. The databases of MEDLINE, Scopus, and Web of Science were searched for studies published in English with no time limitation. Search terms were categorized under three broader concepts: "Digital Health Technologies," "Health and Care," and "Carbon Footprint." Studies were included based on screening titles and abstracts, followed by review of full-text papers by at least 2 reviewers. Narrative synthesis was used to report the findings. RESULTS: The database search yielded 4274 studies. Following screening and reference list checks, 70 studies were selected for inclusion. The earliest included study was published in 2007, with the majority originating from the United States (n=26) and the United Kingdom (n=12). Most studies assessed telemedicine or telehealth interventions. Retrospective analyses predominated, although prospective, cross-sectional, mixed method, and life cycle assessment (LCA) designs were also reported. Environmental impact was most often evaluated through travel-related carbon savings, with some studies incorporating energy use, medical equipment or resources, infrastructure, or waste. LCA studies applied process-based approaches that included detailed activity- and product-specific data, as well as Environmentally Extended Input-Output (EEIO) models. In most cases, carbon footprint measurements were based on assumptions, with calculations relying on published emission factors or calculators. Some studies have expressed CO((2)) emissions using emission equivalencies, such as equivalent carbon sequestration and equivalent CO((2)) emissions. Overall, DHTs demonstrated positive environmental impacts by reducing carbon footprint. However, negative impacts, especially electricity consumption and the lifecycle impacts of digital devices, were also noted. CONCLUSIONS: DHTs are associated with reductions in carbon emissions and related environmental benefits, with telemedicine showing potential to lower the carbon footprint of the health care sector. However, evidence highlights the negative impacts associated with energy demand and the full lifecycle of digital devices. Given the substantial heterogeneity in assessment methods and outcome measures across studies, these findings should be interpreted with caution. To capture the true environmental implications of digital health, more rigorous, standardized, and comprehensive assessment methods are required. Bridging these methodological gaps is essential to ensure that DHTs not only support sustainable health care delivery but also make a meaningful contribution to mitigating global climate change. CI - (c) Sarath Rathnayake, Chinasa Odo, Natasha Alvarado, Hadiza Ismaila, Veronica Parisi, Joshua Pink, Ruth Agbakoba, Rebecca Randell. Originally published in the Journal of Medical Internet Research (https://www.jmir.org). FAU - Rathnayake, Sarath AU - Rathnayake S AUID- ORCID: 0000-0003-2379-1610 AD - Centre for Digital Innovations in Health and Social Care, Institute of Health and Social Care, University of Bradford, Bradford, England, BD7 1DP, United Kingdom, +44 1274236181. AD - Wolfson Centre for Applied Health Research, Bradford, United Kingdom. FAU - Odo, Chinasa AU - Odo C AUID- ORCID: 0000-0002-0770-0806 AD - Centre for Digital Innovations in Health and Social Care, Institute of Health and Social Care, University of Bradford, Bradford, England, BD7 1DP, United Kingdom, +44 1274236181. AD - Wolfson Centre for Applied Health Research, Bradford, United Kingdom. FAU - Alvarado, Natasha AU - Alvarado N AUID- ORCID: 0000-0001-9422-4483 AD - Centre for Digital Innovations in Health and Social Care, Institute of Health and Social Care, University of Bradford, Bradford, England, BD7 1DP, United Kingdom, +44 1274236181. AD - Wolfson Centre for Applied Health Research, Bradford, United Kingdom. FAU - Ismaila, Hadiza AU - Ismaila H AUID- ORCID: 0009-0004-5852-1543 AD - Centre for Digital Innovations in Health and Social Care, Institute of Health and Social Care, University of Bradford, Bradford, England, BD7 1DP, United Kingdom, +44 1274236181. AD - Wolfson Centre for Applied Health Research, Bradford, United Kingdom. FAU - Parisi, Veronica AU - Parisi V AD - Centre for Digital Innovations in Health and Social Care, Institute of Health and Social Care, University of Bradford, Bradford, England, BD7 1DP, United Kingdom, +44 1274236181. AD - Wolfson Centre for Applied Health Research, Bradford, United Kingdom. FAU - Pink, Joshua AU - Pink J AUID- ORCID: 0000-0002-7597-4198 AD - Centre for Digital Innovations in Health and Social Care, Institute of Health and Social Care, University of Bradford, Bradford, England, BD7 1DP, United Kingdom, +44 1274236181. AD - Wolfson Centre for Applied Health Research, Bradford, United Kingdom. FAU - Agbakoba, Ruth AU - Agbakoba R AD - Centre for Digital Innovations in Health and Social Care, Institute of Health and Social Care, University of Bradford, Bradford, England, BD7 1DP, United Kingdom, +44 1274236181. FAU - Randell, Rebecca AU - Randell R AUID- ORCID: 0000-0002-5856-4912 AD - Centre for Digital Innovations in Health and Social Care, Institute of Health and Social Care, University of Bradford, Bradford, England, BD7 1DP, United Kingdom, +44 1274236181. AD - Wolfson Centre for Applied Health Research, Bradford, United Kingdom. LA - eng PT - Journal Article PT - Review PT - Scoping Review DEP - 20261021 PL - Canada TA - J Med Internet Res JT - Journal of medical Internet research JID - 100959882 SB - IM MH - *Carbon Footprint MH - Digital Health MH - Humans MH - *Environment MH - *Digital Technology OTO - NOTNLM OT - carbon footprinting OT - digital technologies OT - environmental impact OT - health care OT - scoping review EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:34 PHST- 2025/11/29 00:00 [received] PHST- 2026/08/13 00:00 [revised] PHST- 2026/08/18 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:34 [entrez] AID - v28i1e88341 [pii] AID - 10.2196/88341 [doi] PST - epublish SO - J Med Internet Res. 2026 Oct 21;28:e88341. doi: 10.2196/88341. PMID- 42954088 OWN - NLM STAT- Publisher LR - 20261029 IS - 1759-9679 (Electronic) IS - 1759-9660 (Linking) DP - 2026 Oct 29 TI - Introduction to electroanalysis. LID - 10.1039/d6ay90128j [doi] AB - Electroanalysis is a powerful tool that underpins a wide range of industrial and academic applications, from pH meters to ion-selective electrodes to glucose meters. It is characterised by rapid measurements, adaptability, miniaturised components and sensitive and specific analysis. However, limited awareness of its capabilities and uncertainty about equipment selection restrict its wider use. This Technical Brief introduces electroanalysis, provides criteria for selecting instrumentation and outlines basic troubleshooting. FAU - Analytical Methods Committee Amctb No AU - Analytical Methods Committee Amctb No LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Anal Methods JT - Analytical methods : advancing methods and applications JID - 101519733 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:33 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:33 [entrez] AID - 10.1039/d6ay90128j [doi] PST - aheadofprint SO - Anal Methods. 2026 Oct 29. doi: 10.1039/d6ay90128j. PMID- 42954081 OWN - NLM STAT- Publisher LR - 20261029 IS - 2040-3372 (Electronic) IS - 2040-3364 (Linking) DP - 2026 Oct 29 TI - A palladium-anchored covalent organic framework for cyclic carbonate and one-pot oxazolidinone synthesis. LID - 10.1039/d6nr03357a [doi] AB - The development of heterogeneous catalysts that efficiently transform carbon dioxide into value-added chemicals under mild conditions offers a sustainable approach towards chemical synthesis. Nevertheless, achieving high catalytic activity, excellent selectivity, and broad applicability within a single catalyst remains a persistent challenge. In this work, an imide-linked covalent organic framework (TAPA-NDA COF) was synthesized and employed as a support for the immobilization of Pd nanoparticles, yielding the Pd@TAPA-NDA COF as an efficient heterogeneous catalyst. The resulting COF-based catalyst was thoroughly characterized by PXRD, FR-IR, FE-SEM, HRTEM, TGA, BET and XPS analyses, confirming the successful formation of the imide-linked framework and the incorporation of Pd species within its porous COF architecture. Furthermore, PXRD analysis provided structural validation of the Pd(6) nanoclusters@TAPA-NDA COF, while theoretical calculations identified the thermodynamically preferred adsorption site of the Pd(6) nanocluster within the COF. The catalytic activity of the Pd@TAPA-NDA COF was first assessed for the cycloaddition of CO(2) with epoxides to form cyclic carbonates, delivering good to excellent yields (i.e. 72% to 96%) with up to 99% selectivity under mild and solvent-free conditions. Subsequently, the catalyst was applied to the cyclization of propargylic alcohols with phenyl isocyanate for the synthesis of 2-oxazolidinones, an important class of bioactive heterocycles, affording the desired products in yields up to 92% with 99% selectivity under the optimized conditions. The outstanding catalytic efficiency is attributed to the synergistic interaction between Pd(0)/Pd(II) active sites and the porous COF. Moreover, the Pd@TAPA-NDA COF could be readily recovered and reused over five consecutive catalytic cycles without any appreciable loss in activity, as evidenced by FT-IR and SEM analyses of the recycled catalyst. Overall, the Pd@TAPA-NDA COF serves as a versatile, recyclable, and efficient heterogeneous catalyst for CO(2) fixation and one pot oxazolidinone synthesis, offering a sustainable platform for value-added chemical synthesis. FAU - Kumar, Susmitha AU - Kumar S AD - Department of Chemistry, University of Kalyani, Kalyani, Nadia-741235, West Bengal, India. manir65@rediffmail.com. FAU - Banerjee, Bipasha AU - Banerjee B AD - Department of Chemistry, University of Kalyani, Kalyani, Nadia-741235, West Bengal, India. manir65@rediffmail.com. FAU - Kumbhakar, Bidhan AU - Kumbhakar B AD - Department of Chemical and Biological Sciences, S.N. Bose National Centre for Basic Sciences, Kolkata, 700106, India. FAU - Ghosh, Swarbhanu AU - Ghosh S AD - Department of Chemistry, McGill University, Montreal, QC, Canada. FAU - Kothari, Anil Chandra AU - Kothari AC AD - Light Stock Processing Division, CSIR Indian Institute of Petroleum, Dehradun, 248005, Uttarakhand, India. AD - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India. FAU - Islam, Sk Manirul AU - Islam SM AUID- ORCID: 0000-0002-3970-5468 AD - Department of Chemistry, University of Kalyani, Kalyani, Nadia-741235, West Bengal, India. manir65@rediffmail.com. FAU - Alothman, Asma A AU - Alothman AA AD - Department of Chemistry, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Nanoscale JT - Nanoscale JID - 101525249 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:33 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:33 [entrez] AID - 10.1039/d6nr03357a [doi] PST - aheadofprint SO - Nanoscale. 2026 Oct 29. doi: 10.1039/d6nr03357a. PMID- 42954073 OWN - NLM STAT- Publisher LR - 20261029 IS - 1754-8411 (Electronic) IS - 1754-8403 (Linking) DP - 2026 Oct 29 TI - Muller glia-derived pseudohypoxia drives intraretinal ischemic vasculopathy and neurovascular dysfunction. LID - dmm.052889 [pii] LID - 10.1242/dmm.052889 [doi] AB - Ischemic retinopathies are characterized by retinal vascular instability, increased vascular permeability, and progressive neurovascular dysfunction. However, the cell-type-specific mechanisms initiating these changes remain insufficiently defined. Muller glia are central regulators of retinal metabolic homeostasis and a major source of vascular endothelial growth factor (VEGF), which positions them as potential upstream drivers of ischemia-associated vascular pathology. Here, we describe a conditional genetic mouse model in which pseudohypoxic signaling is selectively induced in Muller glia through tamoxifen-inducible deletion of von Hippel-Lindau (Vhl) using an Slc1a3-CreER driver. Muller glia-targeted Vhl deletion resulted in robust upregulation of retinal VEGF, intraretinal microvascular abnormalities, focal vascular leakage, corneal hemorrhaging, and microaneurysms. Functional assessment revealed transient inner retinal electroretinographic deficits without overt neuronal loss, consistent with early neurovascular dysfunction. Collectively, these findings demonstrate that glial hypoxia signaling via Vhl knockout is sufficient to induce retinal ischemic vasculopathy reflective of clinical phenotypes, providing a tractable platform for dissecting glial contributions to retinal vascular disease. CI - (c) 2026. Published by The Company of Biologists. FAU - Hu, Hannah F AU - Hu HF AUID- ORCID: 0009-0007-8379-2865 AD - Jonas Children's Vision Care and Bernard & Shirlee Brown Glaucoma Laboratory, Institute of Human Nutrition, Columbia Stem Cell Initiative, New York, NY, USA. AD - Department of Biomedical Engineering, Columbia University, New York, NY, USA. AD - Edward S. Harkness Eye Institute, Columbia University Irving Medical Center, New York-Presbyterian Hospital, New York, NY, USA. AD - Departments of Ophthalmology, Pathology & Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY, USA. FAU - Yun, Justin AU - Yun J AD - Jonas Children's Vision Care and Bernard & Shirlee Brown Glaucoma Laboratory, Institute of Human Nutrition, Columbia Stem Cell Initiative, New York, NY, USA. AD - Department of Biomedical Engineering, Columbia University, New York, NY, USA. AD - Edward S. Harkness Eye Institute, Columbia University Irving Medical Center, New York-Presbyterian Hospital, New York, NY, USA. AD - Departments of Ophthalmology, Pathology & Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY, USA. FAU - Demirkol, Aykut AU - Demirkol A AD - Jonas Children's Vision Care and Bernard & Shirlee Brown Glaucoma Laboratory, Institute of Human Nutrition, Columbia Stem Cell Initiative, New York, NY, USA. AD - Edward S. Harkness Eye Institute, Columbia University Irving Medical Center, New York-Presbyterian Hospital, New York, NY, USA. AD - Vocational School of Health Services, Uskudar University, 34672 Istanbul, Turkey. AD - Departments of Ophthalmology, Pathology & Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY, USA. FAU - Cui, Xuan AU - Cui X AD - Jonas Children's Vision Care and Bernard & Shirlee Brown Glaucoma Laboratory, Institute of Human Nutrition, Columbia Stem Cell Initiative, New York, NY, USA. AD - Edward S. Harkness Eye Institute, Columbia University Irving Medical Center, New York-Presbyterian Hospital, New York, NY, USA. AD - Departments of Ophthalmology, Pathology & Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY, USA. AD - New England College of Optometry, Boston, MA, USA. FAU - Liu, Siyuan AU - Liu S AD - Jonas Children's Vision Care and Bernard & Shirlee Brown Glaucoma Laboratory, Institute of Human Nutrition, Columbia Stem Cell Initiative, New York, NY, USA. AD - Department of Biomedical Engineering, Columbia University, New York, NY, USA. AD - Edward S. Harkness Eye Institute, Columbia University Irving Medical Center, New York-Presbyterian Hospital, New York, NY, USA. AD - Departments of Ophthalmology, Pathology & Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY, USA. FAU - Tsang, Stephen H AU - Tsang SH AD - Jonas Children's Vision Care and Bernard & Shirlee Brown Glaucoma Laboratory, Institute of Human Nutrition, Columbia Stem Cell Initiative, New York, NY, USA. AD - Department of Biomedical Engineering, Columbia University, New York, NY, USA. AD - Edward S. Harkness Eye Institute, Columbia University Irving Medical Center, New York-Presbyterian Hospital, New York, NY, USA. AD - Departments of Ophthalmology, Pathology & Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY, USA. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Dis Model Mech JT - Disease models & mechanisms JID - 101483332 SB - IM OTO - NOTNLM OT - Ischemic retinopathy OT - Muller glial cells OT - Preclinical Mouse Model EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:32 PHST- 2026/02/19 00:00 [received] PHST- 2026/10/24 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:32 [entrez] AID - 373323 [pii] AID - 10.1242/dmm.052889 [doi] PST - aheadofprint SO - Dis Model Mech. 2026 Oct 29:dmm.052889. doi: 10.1242/dmm.052889. PMID- 42954072 OWN - NLM STAT- Publisher LR - 20261029 IS - 1096-9098 (Electronic) IS - 0022-4790 (Linking) DP - 2026 Oct 29 TI - Gastrointestinal Cancer Surgery in Patients on Maintenance Hemodialysis: A Prisma Guided Systematic Review and Propensity Score Matched Cohort Analysis. LID - 10.1002/jso.70438 [doi] AB - BACKGROUND: Patients receiving maintenance hemodialysis (HD) have limited physiologic reserve and high competing cardiometabolic risk, yet surgery remains central to cure for many gastrointestinal (GI) malignancies. Evidence describing oncologic surgery in this population is fragmented across cancer sites and data sources. METHODS: A PRISMA guided systematic review of adult patients on maintenance HD undergoing resection for GI malignancy was performed. The review searched PubMed, Embase, Web of Science, and CENTRAL through March 2026 and included comparative observational studies reporting perioperative or long-term outcomes. In parallel, a propensity-score matched TriNetX cohort of adults undergoing major GI cancer surgery between 2010 and 2025. HD was defined by preoperative ESRD and dialysis dependence codes. Patients were matched 1:1 on age, sex, race, and ethnicity. The primary outcome was 30-day mortality; secondary outcomes were 30-day sepsis, pneumonia, and acute myocardial infarction (AMI). RESULTS: The review included 20 studies after screening 1,744 deduplicated records and 83 full texts. Colorectal surgery provided the most robust evidence: dialysis was associated with markedly higher mortality and serious morbidity, and a published meta-analysis of colorectal surgery estimated a pooled mortality odds ratio (OR) of 4.12. Gastric, pancreatic, and hepatobiliary reports were smaller but showed the same direction of effect, with frequent infectious and cardiovascular complications and substantial competing non-cancer mortality. In the current TriNetX analysis, 3,757 HD patients were matched to 3757 non-HD controls. Thirty-day mortality was 11.4% in HD patients versus 3.0% in controls (OR: 4.21, 95% CI: 3.40-5.22). HD patients also had higher rates of sepsis (14.1% vs. 3.9%; OR 4.06), pneumonia (8.1% vs. 2.2%; OR: 4.02), and AMI (4.3% vs. 0.9%; OR: 5.18). CONCLUSIONS: Across GI cancer operations, maintenance HD identifies a very high-risk surgical phenotype. The dominant short-term pattern is excess death, infection, and cardiopulmonary morbidity; the dominant long-term pattern is competing non-cancer mortality. Surgery remains appropriate for selected patients with resectable disease, but perioperative dialysis timing, multidisciplinary optimization, and treatment in experienced centers are critical. CI - (c) 2026 The Author(s). Journal of Surgical Oncology published by Wiley Periodicals LLC. FAU - Abdelhady, Ali AU - Abdelhady A AUID- ORCID: 0000-0001-9510-6278 AD - Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah, USA. FAU - Sakr, Yara AU - Sakr Y AUID- ORCID: 0000-0002-2838-0768 AD - Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah, USA. FAU - Cardoso, Rayner P AU - Cardoso RP AUID- ORCID: 0009-0006-8726-7550 AD - Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah, USA. FAU - Zineldin, Eman AU - Zineldin E AUID- ORCID: 0000-0001-7257-3033 AD - Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah, USA. FAU - Scaife, Courtney AU - Scaife C AUID- ORCID: 0000-0003-2497-4961 AD - Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah, USA. FAU - Baker, Marshall AU - Baker M AUID- ORCID: 0000-0003-4310-5948 AD - Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah, USA. FAU - Ward, Erin AU - Ward E AUID- ORCID: 0000-0002-0816-1699 AD - Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah, USA. FAU - Gamblin, T Clark AU - Gamblin TC AUID- ORCID: 0000-0001-7580-9961 AD - Huntsman Cancer Institute, University of Utah, Salt Lake City, Utah, USA. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Surg Oncol JT - Journal of surgical oncology JID - 0222643 SB - IM OTO - NOTNLM OT - TriNetX OT - colorectal surgery OT - gastric cancer OT - gastrointestinal cancer OT - hemodialysis OT - postoperative mortality OT - propensity score matching EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:32 PHST- 2026/09/22 00:00 [revised] PHST- 2026/03/26 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:32 [entrez] AID - 10.1002/jso.70438 [doi] PST - aheadofprint SO - J Surg Oncol. 2026 Oct 29. doi: 10.1002/jso.70438. PMID- 42954065 OWN - NLM STAT- Publisher LR - 20261029 IS - 1536-4801 (Electronic) IS - 0277-2116 (Linking) DP - 2026 Oct 29 TI - Digital stool color screening for biliary atresia-Comparing a deep learning approach to the analogue stool color card. LID - 10.1002/jpn3.70615 [doi] AB - OBJECTIVES: Early diagnosis of Biliary atresia (BA) improves native liver and overall survival. Pale stools are a characteristic symptom and might be detected by deep learning image recognition. We tested convolutional neural networks (CNNs) to classify pictures of pale and healthy stools and compare the results with stool color card (SCC) assessment of health care professionals (HCP) and non-health care professionals (NHCP). METHODS: One thousand six hundred and sixteen stool photos with diagnostic labels including BA and healthy controls were graded as pale or normal according to the SCC. A subset of photos (33 BA and 241 healthy) was presented to HCP and NHCP for SCC assessment to evaluate accuracy and interobserver reliability. For CNN model selection and training all images were split into training, validation, and test sets. Different visualization strategies and secondary data augmentation were used for explainability and estimating robustness. RESULTS: In SCC assessment, sensitivity by NHCP trended lower than HCP (86.4% vs. 97.3%), while there were no differences in specificity. Intra-cohort agreement on classification of BA stools (pale vs. normal) was also lower for NHCP than for HCP (36.4% vs. 78.8%), while no significant difference was observed in healthy stools. The selected CNN (ResNet18) correctly classified 97.5% of pale and 98.5% of normal labeled stools, also showing robust results in explainability methods. CONCLUSIONS: The high accuracy of CNN shows the potential for a digital stool color screening. In contrast, NHCP assessment of pale stools tends to be inconsistent, and there is high interobserver variability among HCP and NHCP. CI - (c) 2026 The Author(s). Journal of Pediatric Gastroenterology and Nutrition published by Wiley Periodicals LLC on behalf of European Society for Pediatric Gastroenterology, Hepatology, and Nutrition and North American Society for Pediatric Gastroenterology, Hepatology, and Nutrition. FAU - Griessmair, Lucas AU - Griessmair L AUID- ORCID: 0000-0001-5835-2728 AD - Department of Pediatric Gastroenterology, Nephrology and Metabolic Medicine, Charite - Universitatsmedizin Berlin, Berlin, Germany. FAU - Krumnow, Christian AU - Krumnow C AD - Center for Biomedical Image and Information Processing (CBMI), HTW Berlin, Berlin, Germany. FAU - Slavetinsky, Christoph AU - Slavetinsky C AD - Paediatric Surgery and Urology, University Children's Hospital Tubingen, Tubingen, Germany. FAU - Grothues, Dirk AU - Grothues D AD - University Children's Hospital Regensburg (KUNO), Regensburg, Germany. FAU - Lainka, Elke AU - Lainka E AD - Department of Pediatric Gastroenterology, Hepatology, and Transplant Medicine, Essen University Hospital, Essen, Germany. FAU - Kathemann, Simone AU - Kathemann S AD - Department of Pediatric Gastroenterology, Hepatology, and Transplant Medicine, Essen University Hospital, Essen, Germany. FAU - Lurz, Eberhard AU - Lurz E AD - Dr. von Hauner Children's Hospital, University Hospital, LMU, Munich, Germany. FAU - Buhrer, Christoph AU - Buhrer C AD - Department of Neonatology, Charite - Universitatsmedizin Berlin, Berlin, Germany. FAU - Madadi-Sanjani, Omid AU - Madadi-Sanjani O AD - Department of Pediatric Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. AD - Department of Pediatric Surgery, Hannover Medical School, Hannover, Germany. FAU - Petersen, Claus AU - Petersen C AD - Department of Pediatric Surgery, Hannover Medical School, Hannover, Germany. FAU - Overberg, Johanna AU - Overberg J AD - Department of Pediatric Gastroenterology, Nephrology and Metabolic Medicine, Charite - Universitatsmedizin Berlin, Berlin, Germany. FAU - Henning, Stephan AU - Henning S AD - Department of Pediatric Gastroenterology, Nephrology and Metabolic Medicine, Charite - Universitatsmedizin Berlin, Berlin, Germany. FAU - Weber, Benedikt AU - Weber B AD - Department of Pediatric Gastroenterology, Nephrology and Metabolic Medicine, Charite - Universitatsmedizin Berlin, Berlin, Germany. FAU - Kalveram, Laura AU - Kalveram L AD - Department of Pediatric Gastroenterology, Nephrology and Metabolic Medicine, Charite - Universitatsmedizin Berlin, Berlin, Germany. FAU - Hufnagl, Peter AU - Hufnagl P AD - Center for Biomedical Image and Information Processing (CBMI), HTW Berlin, Berlin, Germany. FAU - Hudert, Christian AU - Hudert C AD - Department of Pediatric Gastroenterology, Nephrology and Metabolic Medicine, Charite - Universitatsmedizin Berlin, Berlin, Germany. FAU - Bufler, Philip AU - Bufler P AD - Department of Pediatric Gastroenterology, Nephrology and Metabolic Medicine, Charite - Universitatsmedizin Berlin, Berlin, Germany. AD - German Center for Child and Adolescent Health (DZKJ), Partner Site Berlin, Berlin, Germany. LA - eng GR - BIH_PRO_609/Stiftung Charite/ PT - Journal Article DEP - 20261029 PL - United States TA - J Pediatr Gastroenterol Nutr JT - Journal of pediatric gastroenterology and nutrition JID - 8211545 SB - IM OTO - NOTNLM OT - artificial intelligence OT - cholestatic liver disease OT - hepatology OT - pediatrics EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:32 PHST- 2026/08/24 00:00 [revised] PHST- 2025/10/14 00:00 [received] PHST- 2026/08/31 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:32 [entrez] AID - 10.1002/jpn3.70615 [doi] PST - aheadofprint SO - J Pediatr Gastroenterol Nutr. 2026 Oct 29. doi: 10.1002/jpn3.70615. PMID- 42954064 OWN - NLM STAT- Publisher LR - 20261029 IS - 2051-6355 (Electronic) IS - 2051-6347 (Linking) DP - 2026 Oct 29 TI - Closing the perovskite loop: aqueous recovery and reuse of MAPbI(3) for film, device, and nanocrystal reuse. LID - 10.1039/d6mh01719c [doi] AB - The rapid commercialisation of organic-inorganic metal halide perovskites (MHPs) presents significant end-of-life challenges, particularly owing to the environmental and health risks their lead content poses. Despite efforts to improve perovskite device performance, comparatively little attention has been given to safe and sustainable recycling, with reported approaches being reliant on hazardous solvents, including DMF. Here, we report the development and use of a water-based solvent for the selective recovery of methylammonium lead iodide (MAPbI(3)) from MHP devices. The solvent comprises of water with low concentrations of dissolved malic acid, beta-alanine, and sodium iodide (NaI), in which the organic additives enable MAPbI(3) dissolution and NaI supports direct MHP recovery. Owing to the temperature dependent solubility of MAPbI(3) in our solvent, high-purity powders can be recovered through precipitation by solvent cooling. Films fabricated from recycled MAPbI(3) exhibit microstructures and bandgaps comparable to those prepared from pristine MAPbI(3), whilst recycled films show improved morphological uniformity and reduced non-radiative recombination. In photoconductor devices, recycled MAPbI(3) films deliver a higher photocurrent density, 1.48 x 10(-5) A cm(-2), and lower dark current density, 1.25 x 10(-7) A cm(-2) compared to their pristine analogues. When incorporated into p-i-n photovoltaic devices, recycled MAPbI(3) films retain >90% of the efficiency of pristine devices. Furthermore, demonstrating the versatility of the solvent system beyond thin-film reuse, MAPbI(3) recovered from thin films is successfully repurposed for the preparation of perovskite nanocrystals. Overall, this work establishes a green and efficient route for the recovery, recycling, and reuse of metal halide perovskites (MHPs), supporting a more circular lifecycle for emerging perovskite optoelectronics. FAU - Du, Zhaoyi AU - Du Z AD - Department of Materials, Molecular Sciences Research Hub, Imperial College London, London, W12 0BZ, UK. FAU - Ding, Ding AU - Ding D AUID- ORCID: 0009-0002-5020-2092 AD - Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, London, W12 0BZ, UK. martyn.mclachlan@imperial.ac.uk. FAU - Li, Longren AU - Li L AUID- ORCID: 0009-0004-2226-0169 AD - Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, London, W12 0BZ, UK. martyn.mclachlan@imperial.ac.uk. FAU - Qian, Weitao AU - Qian W AUID- ORCID: 0009-0007-1840-0343 AD - Department of Materials, Molecular Sciences Research Hub, Imperial College London, London, W12 0BZ, UK. FAU - Tran, Enny AU - Tran E AUID- ORCID: 0000-0002-0155-6663 AD - Department of Materials, Molecular Sciences Research Hub, Imperial College London, London, W12 0BZ, UK. AD - Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, London, W12 0BZ, UK. martyn.mclachlan@imperial.ac.uk. FAU - Shaffer, Milo S P AU - Shaffer MSP AUID- ORCID: 0000-0001-9384-9043 AD - Department of Materials, Molecular Sciences Research Hub, Imperial College London, London, W12 0BZ, UK. AD - Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, London, W12 0BZ, UK. martyn.mclachlan@imperial.ac.uk. FAU - Bakulin, Artem A AU - Bakulin AA AUID- ORCID: 0000-0002-3998-2000 AD - Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, London, W12 0BZ, UK. martyn.mclachlan@imperial.ac.uk. FAU - Gasparini, Nicola AU - Gasparini N AUID- ORCID: 0000-0002-3226-8234 AD - Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, London, W12 0BZ, UK. martyn.mclachlan@imperial.ac.uk. FAU - McLachlan, Martyn A AU - McLachlan MA AUID- ORCID: 0000-0003-3136-1661 AD - Department of Materials, Molecular Sciences Research Hub, Imperial College London, London, W12 0BZ, UK. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Mater Horiz JT - Materials horizons JID - 101623537 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:32 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:32 [entrez] AID - 10.1039/d6mh01719c [doi] PST - aheadofprint SO - Mater Horiz. 2026 Oct 29. doi: 10.1039/d6mh01719c. PMID- 42954063 OWN - NLM STAT- Publisher LR - 20261029 IS - 1096-9098 (Electronic) IS - 0022-4790 (Linking) DP - 2026 Oct 29 TI - Comparative Outcomes of Transoral Robotic and Open Thyroidectomy in Differentiated Thyroid Carcinoma < 2 cm: A Propensity Score-Matched Study. LID - 10.1002/jso.70436 [doi] AB - BACKGROUND: Transoral robotic thyroidectomy (TORT) is a promising alternative to open thyroidectomy (OT) for managing differentiated thyroid carcinoma (DTC), especially in select low-risk patients. This study aimed to compare TORT and OT in patients with DTC < 2 cm using propensity score matching. METHODS: A retrospective analysis of 355 patients who underwent thyroidectomy between April 2020 and August 2024 was conducted. Of these, 100 underwent TORT and 255 underwent OT. Propensity score matching (1:1) yielded 70 patients per group. Outcomes included operative time, lymph node retrieval, complications, and hospital stay. RESULTS: TORT had significantly longer operative times than OT (151.2 +/- 42.1 vs. 48.3 +/- 11.1 min; p < 0.001). Central lymph node retrieval was comparable (4.5 +/- 3.7 vs. 4.4 +/- 3.5 nodes; p = 0.975). No cases of permanent vocal cord palsy occurred in either group. Transient hypoparathyroidism occurred in 3 TORT and 2 OT patients; permanent hypoparathyroidism in 1 TORT patient only (p > 0.05). Hospital stay was slightly longer for TORT (2.1 +/- 0.7 vs. 1.8 +/- 0.7 days; p = 0.011). No recurrences were observed during the median follow-up period of 29 months in the TORT group and 44 months in the OT group (p = 0.238). CONCLUSIONS: TORT demonstrated comparable short-term oncologic and surgical outcomes to OT in well-selected patients with DTC < 2 cm. Further studies with longer follow-up are needed to confirm long-term oncologic safety. CI - (c) 2026 Wiley Periodicals LLC. FAU - Cho, Hyun Soo AU - Cho HS AUID- ORCID: 0009-0007-7490-022X AD - Department of Surgery, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, South Korea. FAU - Oh, Moon Young AU - Oh MY AUID- ORCID: 0000-0002-8939-1735 AD - Department of Surgery, Seoul National University College of Medicine, Seoul Metropolitan Government - Seoul National University Boramae Medical Center, Seoul, South Korea. FAU - Han, Mira AU - Han M AD - Medical Research Collaborating Center, Seoul Metropolitan Government Seoul National University Boramae Medical Center, Seoul, South Korea. FAU - Kim, Young A AU - Kim YA AD - Department of Pathology, Seoul National University College of Medicine, Seoul Metropolitan Government - Seoul National University Boramae Medical Center, Seoul, South Korea. FAU - Chai, Young Jun AU - Chai YJ AUID- ORCID: 0000-0001-8830-3433 AD - Department of Surgery, Seoul National University College of Medicine, Seoul Metropolitan Government - Seoul National University Boramae Medical Center, Seoul, South Korea. AD - Transdisciplinary Department of Medicine & Advanced Technology, Seoul National University Hospital, Seoul, South Korea. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Surg Oncol JT - Journal of surgical oncology JID - 0222643 SB - IM OTO - NOTNLM OT - differentiated thyroid carcinoma OT - minimally invasive surgery OT - open thyroidectomy OT - propensity score matching OT - transoral robotic thyroidectomy EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:32 PHST- 2026/07/27 00:00 [revised] PHST- 2025/11/24 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:32 [entrez] AID - 10.1002/jso.70436 [doi] PST - aheadofprint SO - J Surg Oncol. 2026 Oct 29. doi: 10.1002/jso.70436. PMID- 42954058 OWN - NLM STAT- Publisher LR - 20261029 IS - 1532-4109 (Electronic) IS - 0360-1234 (Linking) DP - 2026 Oct 29 TI - Mechanisms by which soil physicochemical environments regulate microbial biocontrol-agent colonization, persistence, and field efficacy. PG - 1-18 LID - 10.1080/03601234.2026.2752913 [doi] AB - Microbial biocontrol agents are usually selected for antagonism, plant growth promotion, or insect/pathogen suppression, yet their field performance is governed by the soil habitat into which they are released. This review synthesizes how soil physicochemical properties regulate colonization, persistence, and efficacy of microbial biocontrol agents used against soilborne pathogens, pests, and related crop constraints. pH, water potential, temperature, aeration, texture, organic matter, nutrient status, salinity, and redox state are treated as filters that determine whether introduced bacteria, fungi, or nematodes can move, attach, grow, form biofilms, sporulate, and express antagonistic traits. Quantitative examples show that high root recovery can coexist with partial disease control, that dryland rhizospheres can favor redox-active biofilm mechanisms, and that exudate chemistry after pathogen attack can increase antagonist colonization. Three comparison tables summarize soil filters, colonization mechanisms, and management levers. The synthesis argues for soil-scenario claims rather than generic product claims: a biocontrol recommendation should specify the soil conditions, monitoring metric, formulation context, and crop stage under which colonization and efficacy were demonstrated. FAU - Zhan, Lichuan AU - Zhan L AD - Shengzhou Agricultural Technology Extension Center, Shengzhou, China. FAU - Wu, Weihong AU - Wu W AD - College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou, China. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - J Environ Sci Health B JT - Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes JID - 7607167 SB - IM OTO - NOTNLM OT - Bacillus OT - Biological control OT - Pseudomonas OT - Trichoderma OT - biofilm OT - field efficacy OT - microbial inoculants OT - rhizosphere competence OT - soilborne pathogens EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:32 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:32 [entrez] AID - 10.1080/03601234.2026.2752913 [doi] PST - aheadofprint SO - J Environ Sci Health B. 2026 Oct 29:1-18. doi: 10.1080/03601234.2026.2752913. PMID- 42954050 OWN - NLM STAT- Publisher LR - 20261029 IS - 1096-9098 (Electronic) IS - 0022-4790 (Linking) DP - 2026 Oct 29 TI - Retroperitoneal and Lateral Pelvic Lymph Node Dissection for Colorectal Cancer Metastases. LID - 10.1002/jso.70443 [doi] AB - BACKGROUND: Colorectal cancer (CRC) metastases to retroperitoneal (RP) and lateral pelvic (LP) lymph nodes are functionally classified as stage IV disease. While metastasectomy is established for select hepatic and pulmonary metastases, the role of lymphadenectomy for nodal disease remains uncertain. This study evaluates the safety, indications, and oncologic outcomes of RP/LP lymph node dissection in CRC. METHODS: A single-center retrospective review identified patients undergoing RP or LP lymphadenectomy for CRC between 2011 and 2024. Patients with peritoneal metastases or incomplete data were excluded. Demographic, operative, and outcome data were analyzed. Associations with nodal positivity, recurrence-free survival (RFS), and overall survival (OS) were assessed using Fisher's exact test and univariate Cox regression. RESULTS: Forty-nine patients underwent lymphadenectomy, 45% for synchronous and 55% for metachronous nodal metastases. Dissection was selective in 61% and formal in 39%, with 86% undergoing concurrent major abdominal procedures. There was no perioperative mortality and dissection-specific morbidity was low (two lymphatic leaks, both after multivisceral resection). Pathologic nodal metastases were confirmed in 59% of cases. Median RFS and OS were 30.2 and 104.9 months, respectively. Node positivity was associated with initial N2 stage but did not affect RFS or OS. Formal lymphadenectomy was associated with shorter RFS, likely reflecting higher disease burden. CONCLUSIONS: RP and LP lymphadenectomy can be safely performed, even with multivisceral resection. Pathologic nodal status did not predict survival, suggesting potential benefit to lymphadenectomy irrespective of final pathology. These findings support its use in carefully selected patients with controlled systemic disease and isolated nodal recurrence. CI - (c) 2026 Wiley Periodicals LLC. FAU - Olson, Kristofor A AU - Olson KA AUID- ORCID: 0000-0002-4514-9146 AD - Department of Surgery, Division of Colorectal Surgery, City of Hope National Medical Center, Duarte, California, USA. AD - Department of Surgery, Division of Surgical Oncology, City of Hope National Medical Center, Duarte, California, USA. FAU - Maniskas, Seija A AU - Maniskas SA AD - Department of Surgery, Division of Colorectal Surgery, City of Hope National Medical Center, Duarte, California, USA. FAU - Marty, Makenna AU - Marty M AD - Department of Surgery, Division of Colorectal Surgery, City of Hope National Medical Center, Duarte, California, USA. FAU - Labadie, Kevin AU - Labadie K AUID- ORCID: 0000-0002-5729-3524 AD - Department of Surgery, Division of Colorectal Surgery, City of Hope National Medical Center, Duarte, California, USA. AD - Department of Surgery, Division of Surgical Oncology, City of Hope National Medical Center, Duarte, California, USA. FAU - Nguyen, Jade AU - Nguyen J AD - Department of Surgery, Division of Colorectal Surgery, City of Hope National Medical Center, Duarte, California, USA. FAU - Zhumkhawala, Ali AU - Zhumkhawala A AD - Department of Surgery, Division of Urology, City of Hope National Medical Center, Duarte, California, USA. FAU - Lai, Lily L AU - Lai LL AUID- ORCID: 0000-0001-6639-4485 AD - Department of Surgery, Division of Colorectal Surgery, City of Hope National Medical Center, Duarte, California, USA. FAU - Fong, Yuman AU - Fong Y AUID- ORCID: 0000-0002-8934-9959 AD - Department of Surgery, Division of Surgical Oncology, City of Hope National Medical Center, Duarte, California, USA. FAU - Kaiser, Andreas M AU - Kaiser AM AUID- ORCID: 0000-0003-4343-5189 AD - Department of Surgery, Division of Colorectal Surgery, City of Hope National Medical Center, Duarte, California, USA. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Surg Oncol JT - Journal of surgical oncology JID - 0222643 SB - IM OTO - NOTNLM OT - colorectal cancer OT - lateral pelvic lymph node OT - lymph node dissection OT - lymphadenectomy OT - para-aortic lymph node OT - retroperitoneal lymph node EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:31 PHST- 2026/10/19 00:00 [revised] PHST- 2026/08/06 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:31 [entrez] AID - 10.1002/jso.70443 [doi] PST - aheadofprint SO - J Surg Oncol. 2026 Oct 29. doi: 10.1002/jso.70443. PMID- 42954049 OWN - NLM STAT- Publisher LR - 20261029 IS - 1759-9679 (Electronic) IS - 1759-9660 (Linking) DP - 2026 Oct 29 TI - Evaluation of a boronate-masked naphthalimide probe for peroxynitrite-responsive fluorescence imaging in experimental acute liver injury. LID - 10.1039/d6ay01756h [doi] AB - Peroxynitrite (ONOO(-)), a key mediator of oxidative and nitrosative stress, participates extensively in the pathological progression of acute liver injury and cancer. Because aberrant ONOO(-) production is closely associated with these conditions, accurate analysis of this short-lived species is important for early diagnosis and for evaluating therapeutic responses. Real-time monitoring of ONOO(-) in representative disease models is therefore valuable for identifying pathophysiological changes before irreversible tissue damage occurs. Fluorescence imaging provides a highly sensitive, noninvasive, convenient, and selective means of detecting and visualizing ONOO(-)in situ within complex biological systems. Here, we developed HD-ONOO, a lysosome-associated fluorescent probe constructed on a naphthalimide scaffold, for ONOO(-)-responsive fluorescence measurements and exploratory biological imaging. The probe responded to added ONOO(-) and showed a low background signal under the tested conditions. Cellular fluorescence experiments demonstrated that HD-ONOO reported fluorescence changes following oxidant exposure and pharmacological modulation in living LX-2 cells. Its biological applicability was further established by whole-animal and tissue-section imaging, which revealed treatment-associated fluorescence in hepatic samples. In particular, the probe reported fluorescence differences in two chemically induced models of acute liver injury and produced trends consistent with organ imaging and serum biochemical indices. This study evaluates an established boronate-based design in experimental liver-injury settings; the fluorescence readout alone does not establish oxidant identity or absolute ONOO(-) concentrations. HD-ONOO may provide an exploratory imaging reagent for studies of hepatic redox responses, subject to direct product identification and further analytical validation. FAU - Li, Ping-Gui AU - Li PG AD - Jiangsu Key Laboratory for Recognition and Remediation of Emerging Pollutants in Taihu Basin, School of Environment Science and Engineering, Wuxi University, Wuxi, 214105, China. lipg@cwxu.edu.cn. FAU - Han, Meng-Ting AU - Han MT AD - Jiangsu Key Laboratory for Recognition and Remediation of Emerging Pollutants in Taihu Basin, School of Environment Science and Engineering, Wuxi University, Wuxi, 214105, China. lipg@cwxu.edu.cn. FAU - Cui, Xin-Ping AU - Cui XP AD - Jiangsu Key Laboratory for Recognition and Remediation of Emerging Pollutants in Taihu Basin, School of Environment Science and Engineering, Wuxi University, Wuxi, 214105, China. lipg@cwxu.edu.cn. FAU - Wang, Tong AU - Wang T AD - Independent Researcher, Private Dental Clinic, Syracuse, New York, USA. FAU - Guo, Rui AU - Guo R AD - College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, China. GR0913@sdau.edu.cn. FAU - Zhang, Bo AU - Zhang B AD - Center of Clinical Research, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center, Nanjing Medical University, Wuxi 214023, China. zhangbo_cpu1713@njmu.edu.cn. AD - Department of Laboratory Medicine, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center, Nanjing Medical University, Wuxi 214023, China. FAU - Wang, Kai AU - Wang K AUID- ORCID: 0000-0002-8326-7178 AD - Department of Chemical and Biomolecular Engineering, National University of Singapore, 117585, Singapore. hyxj0309@163.com. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Anal Methods JT - Analytical methods : advancing methods and applications JID - 101519733 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:31 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:31 [entrez] AID - 10.1039/d6ay01756h [doi] PST - aheadofprint SO - Anal Methods. 2026 Oct 29. doi: 10.1039/d6ay01756h. PMID- 42954047 OWN - NLM STAT- Publisher LR - 20261029 IS - 1096-9098 (Electronic) IS - 0022-4790 (Linking) DP - 2026 Oct 29 TI - Patterns and Predictors of Relapse Following Cytoreductive Surgery for Pseudomyxoma Peritonei. LID - 10.1002/jso.70445 [doi] AB - OBJECTIVE: To evaluate the incidence, timing and anatomical pattern of relapse after cytoreductive surgery (CRS) for pseudomyxoma peritonei (PMP), and identify predictors of overall and early (within 12 months) relapse. METHODS: Retrospective cohort study of consecutive patients undergoing curative-intent CRS for appendiceal PMP at a single institution (2016-2025), recording the site of first relapse. Predictors of progression-free survival (PFS) and early relapse were evaluated using Cox and logistic regression. RESULTS: Among 204 patients, CC-0/1 cytoreduction was achieved in 89% and HIPEC in 92%. At 37.1-month median follow-up, 89 patients (44%) relapsed; 3-year PFS and overall survival were 50.2% and 75.6%. Relapse was predominantly intraperitoneal, most often the pelvis and small bowel/mesentery (25% each). Histology and completeness of cytoreduction independently predicted PFS: low-grade (HR 2.18), high-grade (HR 2.79) and signet-ring PMP (HR 4.35) vs acellular mucin, CC-2/3 (HR 2.18), and male sex (HR 1.74). Early relapse occurred in 34 patients (17%), independently predicted by high-grade histology (OR 5.06) and CC-2/3 (OR 3.55). CONCLUSION: Relapse after CRS for PMP is predominantly intraperitoneal, most often the small bowel/mesentery and pelvis. Histology and completeness of cytoreduction are the principal determinants of overall and early relapse, supporting risk-stratified surveillance and selective repeat cytoreduction. CI - (c) 2026 Wiley Periodicals LLC. FAU - Kazi, Mufaddal AU - Kazi M AUID- ORCID: 0000-0002-3295-0006 AD - Department of Surgical Oncology, Advanced Centre for Treatment, Research, and Education in Cancer, ACTREC, Mumbai, Maharashtra, India. AD - Department of Surgical Oncology, Tata Memorial Hospital, Dr. E Borges Road, Mumbai, Maharashtra, India. FAU - Reddy, Kaliki Sailesh AU - Reddy KS AUID- ORCID: 0009-0006-2418-7182 AD - Department of Surgical Oncology, Tata Memorial Hospital, Dr. E Borges Road, Mumbai, Maharashtra, India. FAU - Sharma, Ankit AU - Sharma A AUID- ORCID: 0000-0002-9212-6844 AD - Department of Surgical Oncology, Advanced Centre for Treatment, Research, and Education in Cancer, ACTREC, Mumbai, Maharashtra, India. AD - Department of Surgical Oncology, Tata Memorial Hospital, Dr. E Borges Road, Mumbai, Maharashtra, India. FAU - De Souza, Ashwin AU - De Souza A AUID- ORCID: 0000-0002-2722-0826 AD - Department of Surgical Oncology, Advanced Centre for Treatment, Research, and Education in Cancer, ACTREC, Mumbai, Maharashtra, India. AD - Department of Surgical Oncology, Tata Memorial Hospital, Dr. E Borges Road, Mumbai, Maharashtra, India. FAU - Saklani, Avanish AU - Saklani A AUID- ORCID: 0000-0003-4498-7612 AD - Department of Surgical Oncology, Advanced Centre for Treatment, Research, and Education in Cancer, ACTREC, Mumbai, Maharashtra, India. AD - Department of Surgical Oncology, Tata Memorial Hospital, Dr. E Borges Road, Mumbai, Maharashtra, India. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - J Surg Oncol JT - Journal of surgical oncology JID - 0222643 SB - IM EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:31 PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/03 00:00 [received] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:31 [entrez] AID - 10.1002/jso.70445 [doi] PST - aheadofprint SO - J Surg Oncol. 2026 Oct 29. doi: 10.1002/jso.70445. PMID- 42954032 OWN - NLM STAT- Publisher LR - 20261029 IS - 1369-1651 (Electronic) IS - 0899-0220 (Linking) DP - 2026 Oct 29 TI - Effects of pathway shape and dual-tasking on spatiotemporal gait parameters in individuals with Parkinson's disease. PG - 1-11 LID - 10.1080/08990220.2026.2751173 [doi] AB - PURPOSE: Examining the effects of different pathway shapes on walking performance, particularly under cognitive load, is important for understanding motor control in individuals with Parkinson's disease (PD) under real-life conditions. This study aimed to investigate the effects of walking on different pathway shapes and dual-tasking (DT) on spatiotemporal gait parameters in individuals with PD. MATERIALS AND METHODS: A prospective cross-sectional observational study was conducted with 50 individuals diagnosed with PD. Participants were initially assessed using the Unified Parkinson's Disease Rating Scale (UPDRS), the Dual-Task Questionnaire (DTQ), and the Modified Gait Efficacy Scale (mGES). Subsequently, spatiotemporal characteristics of the 4-Meter Walk Test (4MWT), Timed Up and Go Test (TUG), Figure-of-8 Walk Test (F8WT), and L Test were measured under single-task and DT conditions. RESULTS: In all tests, dual-task conditions significantly increased duration and step count (p < 0.05). The F8WT showed multiple spatiotemporal impairments (p < 0.05). The highest duration and step count costs were in the 4-MWT (p < 0.001), while the highest speed and step length costs were in the TUG (p /= 0.307,p < 0.05), while F8WT cadence (r= -0.310, p = 0.034) and TUG duration (r= -0.426,p = 0.003) correlated with UPDRS-III and mGES, respectively. DTQ selectively correlated with speed and length across tests (p < 0.05). CONCLUSIONS: Dual-task conditions impair both straight and shaped walking in PD. While 4-MWT exhibits higher time-space costs, DTQ and UPDRS-III selectively determine dual-task performance across different pathway shapes. Integrating both straight and curved paths under cognitive load provides a more comprehensive approach for clinical assessment and rehabilitation. FAU - Ozden, Fatih AU - Ozden F AUID- ORCID: 0000-0001-6593-3758 AD - Koycegiz Vocational School of Health Services, Department of Health Care Services, Mugla Sitki Kocman University, Mugla, Turkiye. FAU - Karakaya, Mehmet Gurhan AU - Karakaya MG AD - Faculty of Health Sciences, Department of Physiotherapy and Rehabilitation, Mugla Sitki Kocman University, Mugla, Turkiye. FAU - Uysal, Ismail AU - Uysal I AUID- ORCID: 0000-0001-9821-2180 AD - Fethiye Vocational School of Health Services, Department of Health Care Services, Mugla Sitki Kocman University, Mugla, Turkiye. FAU - Tumturk, Ismet AU - Tumturk I AD - Faculty of Health Sciences, Department of Physiotherapy and Rehabilitation, Antalya Bilim University, Antalya, Turkiye. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Somatosens Mot Res JT - Somatosensory & motor research JID - 8904127 SB - IM OTO - NOTNLM OT - Cognitive task OT - Parkinsonism OT - gait analysis EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:30 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:30 [entrez] AID - 10.1080/08990220.2026.2751173 [doi] PST - aheadofprint SO - Somatosens Mot Res. 2026 Oct 29:1-11. doi: 10.1080/08990220.2026.2751173. PMID- 42954031 OWN - NLM STAT- Publisher LR - 20261029 IS - 1613-6829 (Electronic) IS - 1613-6810 (Linking) DP - 2026 Oct 29 TI - Amorphous Co(2)O(3)/Crystalline Cu Interface Enables Ultra-Rapid Nitrite Conversion for Integrated Cascade Electroreduction of Nitrate to Ammonia. LID - e76475 [pii] LID - 10.1002/smll.76475 [doi] AB - Given serious nitrate (NO(3) (-)) pollution and the energy-intensive nature of traditional ammonia (NH(3)) synthesis, the electrocatalytic NO(3) (-) reduction reaction (NO(3) (-)RR) offers a green route for NH(3) production. Copper (Cu)-based catalysts, although kinetically favorable for NO(3) (-)-to-NO(2) (-) conversion, still suffer from sluggish *NO(2) reduction, resulting in poor NH(3) selectivity. Herein, a tandem catalysis strategy is adopted to fabricate a cobalt(III) oxide-Cu heterostructure on nickel foam (Co(2)O(3)-Cu/NF) to enhance NO(3) (-)RR activity. Coordination distortion and asymmetry confirm the distinctive amorphous nature of Co(2)O(3). Results from online differential electrochemical mass spectrometry, electron paramagnetic resonance, and theoretical calculations indicate that Cu dominates the conversion of NO(3) (-) to NO(2) (-), while Co sites work in tandem to optimize the NO(2) (-) adsorption, reduce the decisive transformation barrier from *NO(2) to *NO, and facilitate water dissociation (producing *H) for N-intermediate hydrogenation. Electrochemical kinetic tests confirm that Co(2)O(3) incorporation promotes the conversion of NO(3) (-) to NO(2) (-) and enhances the deep hydrogenation capability for rapidly transforming NO(2) (-) into other intermediates. Co(2)O(3)-Cu/NF achieves a high NH(3) yield rate of 90.45 +/- 0.76 mg h(-1) cm(-2) and NH(3) Faradaic efficiency (FE-NH(3)) of 93.4% +/- 2.5%. This work overcomes the inherent limitations of Cu-based catalysts through crystalline-amorphous dual-site tandem synergy, offering new insights for developing NO(3) (-)RR technology. CI - (c) 2026 Wiley-VCH GmbH. FAU - Cui, Wenjing AU - Cui W AD - Heilongjiang Provincial Key Laboratory of Environmental Nanotechnology and Key Laboratory of Functional Inorganic Material Chemistry, School of Chemistry and Materials Science, Ministry of Education of the People's Republic of China, Heilongjiang University, Harbin, China. FAU - Chen, Yanjie AU - Chen Y AD - Heilongjiang Provincial Key Laboratory of Environmental Nanotechnology and Key Laboratory of Functional Inorganic Material Chemistry, School of Chemistry and Materials Science, Ministry of Education of the People's Republic of China, Heilongjiang University, Harbin, China. FAU - Huo, Sichen AU - Huo S AD - Heilongjiang Provincial Key Laboratory of Environmental Nanotechnology and Key Laboratory of Functional Inorganic Material Chemistry, School of Chemistry and Materials Science, Ministry of Education of the People's Republic of China, Heilongjiang University, Harbin, China. FAU - Cai, Zhuang AU - Cai Z AD - Heilongjiang Provincial Key Laboratory of Environmental Nanotechnology and Key Laboratory of Functional Inorganic Material Chemistry, School of Chemistry and Materials Science, Ministry of Education of the People's Republic of China, Heilongjiang University, Harbin, China. FAU - Shi, Shuang AU - Shi S AD - Heilongjiang Provincial Key Laboratory of Environmental Nanotechnology and Key Laboratory of Functional Inorganic Material Chemistry, School of Chemistry and Materials Science, Ministry of Education of the People's Republic of China, Heilongjiang University, Harbin, China. FAU - Guan, Huanyu AU - Guan H AD - Heilongjiang Provincial Key Laboratory of Environmental Nanotechnology and Key Laboratory of Functional Inorganic Material Chemistry, School of Chemistry and Materials Science, Ministry of Education of the People's Republic of China, Heilongjiang University, Harbin, China. FAU - Wang, Xinyu AU - Wang X AD - Heilongjiang Provincial Key Laboratory of Environmental Nanotechnology and Key Laboratory of Functional Inorganic Material Chemistry, School of Chemistry and Materials Science, Ministry of Education of the People's Republic of China, Heilongjiang University, Harbin, China. FAU - Li, Jitao AU - Li J AD - School of Civil Engineering, Heilongjiang Institute of Technology, Harbin, China. FAU - Gu, Peng AU - Gu P AD - Heilongjiang Provincial Key Laboratory of Environmental Nanotechnology and Key Laboratory of Functional Inorganic Material Chemistry, School of Chemistry and Materials Science, Ministry of Education of the People's Republic of China, Heilongjiang University, Harbin, China. FAU - Zou, Jinlong AU - Zou J AUID- ORCID: 0000-0003-0651-761X AD - Heilongjiang Provincial Key Laboratory of Environmental Nanotechnology and Key Laboratory of Functional Inorganic Material Chemistry, School of Chemistry and Materials Science, Ministry of Education of the People's Republic of China, Heilongjiang University, Harbin, China. FAU - Dai, Ying AU - Dai Y AD - School of Civil Engineering, Heilongjiang Institute of Technology, Harbin, China. FAU - Wang, Lei AU - Wang L AUID- ORCID: 0000-0002-0624-5098 AD - Heilongjiang Provincial Key Laboratory of Environmental Nanotechnology and Key Laboratory of Functional Inorganic Material Chemistry, School of Chemistry and Materials Science, Ministry of Education of the People's Republic of China, Heilongjiang University, Harbin, China. LA - eng GR - 52470073/National Natural Science Foundation of China/ GR - 52070074/National Natural Science Foundation of China/ GR - 2022ZX07D04/Heilongjiang Province Key Research and Development Plan/ GR - PL2024B021/Natural Science Foundation of Heilongjiang Province/ GR - LJGXCG2024-P02/Cooperative Innovation Achievement Project of 'Double First-Class' Disciplines in Heilongjiang Province/ PT - Journal Article DEP - 20261029 PL - Germany TA - Small JT - Small (Weinheim an der Bergstrasse, Germany) JID - 101235338 SB - IM OTO - NOTNLM OT - ammonia OT - hydrogenation OT - interface regulation OT - nitrate reduction OT - tandem catalysis EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:30 PHST- 2026/10/12 00:00 [revised] PHST- 2026/06/11 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:30 [entrez] AID - e76475 [pii] AID - 10.1002/smll.76475 [doi] PST - aheadofprint SO - Small. 2026 Oct 29:e76475. doi: 10.1002/smll.76475. PMID- 42954030 OWN - NLM STAT- Publisher LR - 20261029 IS - 1748-6963 (Electronic) IS - 1743-5889 (Linking) DP - 2026 Oct 29 TI - Hybrid lipid-coated silver nanoparticles as programmable antimicrobial platforms: balancing silver accessibility, efficacy, and toxicity. PG - 1-11 LID - 10.1080/17435889.2026.2752933 [doi] AB - Silver nanoparticles (AgNPs) can deliver reactive silver, but the processes that drive antimicrobial activity can also injure host cells. Lipid interfaces may modify this balance by regulating nanoparticle transformation and interactions with biological targets. This Special Report examines sodium oleate-phosphatidylcholine-alkanethiol hybrid lipid membranes (HLMs) as a case study of interfacial control, with selected alternative coatings providing comparative context. Silver accessibility is defined as a time-, environment-, and target-dependent property that requires separate measurements of silver species, nanoparticle interactions, and coating evolution. Existing HLM studies demonstrate physicochemical control and changes in developmental vertebrate or environmental toxicity under defined conditions; these findings do not establish improved antimicrobial therapeutic benefit. Representative antimicrobial and mammalian-cell studies using other coatings illustrate both the value and limitations of efficacy-toxicity comparisons. Progress requires paired biological measurements, direct assessment of coating and corona evolution, and independent validation in relevant formulations. Dental, wound, device, and intracellular uses are considered as distinct evidence needs rather than established HLM therapies. FAU - Schulte, Megan C AU - Schulte MC AD - Department of Chemistry, Oregon State University, Corvallis, OR, USA. FAU - Patel, Priyal D AU - Patel PD AD - Department of Chemistry, Oregon State University, Corvallis, OR, USA. FAU - Sheehan, Genevive L AU - Sheehan GL AD - Department of Chemistry, Oregon State University, Corvallis, OR, USA. FAU - Mackiewicz, Marilyn R AU - Mackiewicz MR AD - Department of Chemistry, Oregon State University, Corvallis, OR, USA. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Nanomedicine (Lond) JT - Nanomedicine (London, England) JID - 101278111 SB - IM OTO - NOTNLM OT - Silver nanoparticles OT - antimicrobial nanomedicine OT - cytotoxicity OT - hybrid lipid membrane OT - lipid coating OT - nano-bio interface OT - silver ion release EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:30 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:30 [entrez] AID - 10.1080/17435889.2026.2752933 [doi] PST - aheadofprint SO - Nanomedicine (Lond). 2026 Oct 29:1-11. doi: 10.1080/17435889.2026.2752933. PMID- 42954014 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2093-0879 (Print) IS - 2093-0879 (Linking) VI - 32 IP - 4 DP - 2026 Oct 30 TI - Effects of Needleless Transcutaneous Electrical Acustimulation on Gastrointestinal Function Recovery After Multi-Level Lumbar Surgery: A Randomized Clinical Trial. PG - 644-654 LID - 10.5056/jnm25261 [doi] AB - BACKGROUND/AIMS: : Postoperative gastrointestinal dysfunction is a common complication of spinal surgery. This study aimed to determine the effect of transcutaneous electrical acustimulation (TEA) on the recovery of postoperative gastrointestinal function in patients undergoing lumbar surgery and to explore the underlying mechanism. METHODS: : Fifty patients undergoing multi-level (n >/= 2) lumbar surgery were randomly assigned to receive TEA or sham-TEA treatment for 1 hour twice daily from postoperative day (POD)1 to POD3. Zusanli (ST36) and Neiguan (PC6) were selected as the stimulation points for the TEA group. The time to first flatus and defecation after surgery and other outcome measures were recorded. Gastric slow waves (GSW) were detected using an electrogastrogram, and autonomic function was assessed by analyzing heart rate variability derived from electrocardiogram recordings. Serum levels of interleukin (IL)-6, serotonin, and norepinephrine (NE) were also examined. RESULTS: : Compared with sham-TEA, TEA significantly shortened the time to first flatus (P < 0.001) and defecation (P < 0.05) and reduced gastrointestinal symptom scores (P < 0.01). TEA increased the percentage of normal GSW (P < 0.05) and vagal activity (P < 0.001) postoperatively. Compared with POD1, TEA decreased the levels of IL-6, serotonin, and NE on POD4. Additionally, vagal activity on POD4 was negatively correlated with the time to first flatus (r = -0.383, P = 0.009). TEA treatment was identified as a factor independently associated with the time to first postoperative flatus and defecation. CONCLUSIONS: : Needleless TEA is probably associated with accelerated recovery of gastrointestinal function after lumbar surgery, possibly through the modulation of autonomic activity. FAU - Song, Yuhao AU - Song Y AUID- ORCID: 0009-0003-6640-1560 AD - Department of Gastroenterology, The Second Affiliated Hospital of Naval Medical University, Shanghai, China. FAU - Wang, Jian AU - Wang J AUID- ORCID: 0000-0002-7281-6338 AD - Department of Gastroenterology, The Second Affiliated Hospital of Naval Medical University, Shanghai, China. FAU - Zhang, Wenyu AU - Zhang W AUID- ORCID: 0000-0003-1693-6009 AD - Spine Center, Department of Orthopedics, The Second Affiliated Hospital of Naval Medical University, Shanghai, China. FAU - Li, Tianxing AU - Li T AUID- ORCID: 0009-0004-3739-1065 AD - Department of Gastroenterology, The Second Affiliated Hospital of Naval Medical University, Shanghai, China. FAU - Mao, Yanan AU - Mao Y AUID- ORCID: 0009-0004-4619-0599 AD - Department of Gastroenterology, The Second Affiliated Hospital of Naval Medical University, Shanghai, China. FAU - Chen, Jiande D Z AU - Chen JDZ AUID- ORCID: 0000-0002-0191-5697 AD - Division of Gastroenterology and Hepatology, University of Michigan, Ann Arbor, MI, USA. FAU - Liang, Lei AU - Liang L AUID- ORCID: 0000-0002-7161-9252 AD - Spine Center, Department of Orthopedics, The Second Affiliated Hospital of Naval Medical University, Shanghai, China. FAU - Xie, Weifen AU - Xie W AUID- ORCID: 0000-0002-7137-112X AD - Department of Gastroenterology, The Second Affiliated Hospital of Naval Medical University, Shanghai, China. FAU - Ning, Beifang AU - Ning B AUID- ORCID: 0009-0003-9629-9755 AD - Department of Gastroenterology, The Second Affiliated Hospital of Naval Medical University, Shanghai, China. LA - eng PT - Journal Article PL - Korea (South) TA - J Neurogastroenterol Motil JT - Journal of neurogastroenterology and motility JID - 101530189 OTO - NOTNLM OT - Autonomic nervous system OT - Electroacupuncture OT - Gastrointestinal motility OT - Lumbar vertebrae OT - Transcutaneous electric nerve stimulation EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:30 PHST- 2025/12/31 00:00 [received] PHST- 2026/03/14 00:00 [revised] PHST- 2026/04/01 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:30 [entrez] AID - jnm25261 [pii] AID - 10.5056/jnm25261 [doi] PST - ppublish SO - J Neurogastroenterol Motil. 2026 Oct 30;32(4):644-654. doi: 10.5056/jnm25261. PMID- 42954006 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2093-0879 (Print) IS - 2093-0879 (Linking) VI - 32 IP - 4 DP - 2026 Oct 30 TI - Acid-Lowering Agents in Functional Dyspepsia: Assessing the Role and Effectiveness of Proton Pump Inhibitors and Beyond. PG - 541-548 LID - 10.5056/jnm25237 [doi] AB - Functional dyspepsia (FD) is a prevalent functional gastrointestinal disorder with complex pathophysiology. While acid exposure is implicated in its pathogenesis, the therapeutic focus is shifting toward understanding the full scope of acid-suppressive therapy. This review aims to assess the role and effectiveness of proton pump inhibitors (PPIs) in FD, with particular emphasis on their mechanisms beyond acid suppression, and to explore the potential of novel acid-lowering agents. We synthesized current evidence from key clinical trials, meta-analyses, and mechanistic studies to evaluate the efficacy of PPIs and to elucidate their non-acid-suppressive effects. Meta-analyses confirm that PPIs are superior to placebo for global symptom relief in FD, with the most consistent benefits observed for epigastric pain and burning. Beyond their well-established acid-suppressive action, PPIs demonstrate significant non-acid-related effects that may contribute to their therapeutic action. These include anti-inflammatory properties, such as reducing duodenal eosinophil and mast cell infiltration, and modulation of gut microbiota and intestinal permeability. However, these mechanisms may also underlie potential long-term adverse effects. Potassium-competitive acid blockers (P-CABs) represent a promising new class of agents with pharmacological advantages, showing efficacy in initial studies, though their superiority over PPIs requires further validation. PPIs remain an evidence-based first-line therapy for FD, supported by proven efficacy and newly discovered pleiotropic mechanisms. However, their application should be individualized, with consideration of symptom subtype and potential long-term consequences. Future management strategies should focus on refined patient selection and the integration of novel agents like P-CABs. FAU - Ma, Ruiqi AU - Ma R AUID- ORCID: 0000-0001-8122-8098 AD - Department of Gastroenterology and Hepatology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. FAU - Xiao, Yinglian AU - Xiao Y AUID- ORCID: 0000-0002-4223-4943 AD - Department of Gastroenterology and Hepatology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. LA - eng PT - Journal Article PT - Review PL - Korea (South) TA - J Neurogastroenterol Motil JT - Journal of neurogastroenterology and motility JID - 101530189 OTO - NOTNLM OT - Functional dyspepsia OT - Non-acid-suppressive effects OT - Potassium-competitive acid blocker OT - Proton pump inhibitors EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:30 PHST- 2025/11/28 00:00 [received] PHST- 2026/02/04 00:00 [revised] PHST- 2026/02/12 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:30 [entrez] AID - jnm25237 [pii] AID - 10.5056/jnm25237 [doi] PST - ppublish SO - J Neurogastroenterol Motil. 2026 Oct 30;32(4):541-548. doi: 10.5056/jnm25237. PMID- 42954000 OWN - NLM STAT- Publisher LR - 20261029 IS - 1097-4598 (Electronic) IS - 0148-639X (Linking) DP - 2026 Oct 29 TI - Feasibility of Longitudinal Home Monitoring of Nocturnal Transcutaneous CO(2) and Relationship to Standard Respiratory Measures in Patients With ALS: A Pilot Cohort Study. LID - 10.1002/mus.70432 [doi] AB - INTRODUCTION/AIMS: Amyotrophic lateral sclerosis (ALS) results in respiratory muscle weakness causing hypoventilation, retention of carbon dioxide (CO(2)), and chronic hypercapnic respiratory failure. In this pilot study, we performed a 12-month assessment of daytime and home nocturnal CO(2) to track respiratory progression, determine relationships with surrogate respiratory measures, and assess the impact of non-invasive ventilatory support. METHODS: Adults with forced vital capacity (FVC) less than 90% predicted secondary to ALS were recruited from two sites in the northeast US. Participants were followed up at standard visits every 3 months for up to five visits. Spirometry, functional scores, and patient-reported outcomes of dyspnea were recorded. Each visit included a 15-min, seated measurement of transcutaneous CO(2) (PtcCO(2)) while in clinic, followed by 3 nights of home overnight PtcCO(2) monitoring. Linear mixed-effects models were employed to describe the changes that occurred longitudinally and resulting from the initiation of non-invasive ventilation (NIV). RESULTS: Sixteen subjects were enrolled, with nine completing the full study. Strength and FVC declined over time, while daytime PtcCO(2) remained stable. Five individuals demonstrated nocturnal hypercapnia that preceded FVC < 50% predicted normal. Transition to NIV was associated with a 7 mmHg decrease in nocturnal PtcCO(2). DISCUSSION: Home nocturnal PtcCO(2) assessment in ALS may identify respiratory insufficiency prior to FVC. Transcutaneous CO(2) may play a role in the respiratory management of patients as a biomarker for hypoventilation risk and NIV titration. However, equipment availability and data quality limitations would benefit from professional assistance rather than independent in-home use. CI - (c) 2026 The Author(s). Muscle & Nerve published by Wiley Periodicals LLC. FAU - Geronimo, Andrew AU - Geronimo A AUID- ORCID: 0000-0002-7754-3754 AD - Department of Neurology, Penn State College of Medicine, Hershey, Pennsylvania, USA. FAU - Cao, Quy AU - Cao Q AD - Department of Biostatistics, Epidemiology and Informatics, University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Elman, Lauren AU - Elman L AD - Department of Neurology, University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Simmons, Zachary AU - Simmons Z AUID- ORCID: 0000-0001-8574-5332 AD - Department of Neurology, Penn State College of Medicine, Hershey, Pennsylvania, USA. FAU - Ackrivo, Jason AU - Ackrivo J AUID- ORCID: 0000-0003-4379-1255 AD - Department of Neurology, University of Pennsylvania, Philadelphia, Pennsylvania, USA. AD - Department of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA. LA - eng GR - MDA874016/Muscular Dystrophy Association/ GR - UL1 TR002014/TR/NCATS NIH HHS/United States GR - UL1 TR00045/TR/NCATS NIH HHS/United States GR - K23 HL-151879/HL/NHLBI NIH HHS/United States GR - Sentec AG/ PT - Journal Article DEP - 20261029 PL - United States TA - Muscle Nerve JT - Muscle & nerve JID - 7803146 SB - IM OTO - NOTNLM OT - capnography OT - hypoventilation OT - remote monitoring OT - sleep OT - spirometry EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:30 PHST- 2026/09/24 00:00 [revised] PHST- 2026/03/17 00:00 [received] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:30 [entrez] AID - 10.1002/mus.70432 [doi] PST - aheadofprint SO - Muscle Nerve. 2026 Oct 29. doi: 10.1002/mus.70432. PMID- 42953986 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2692-9368 (Electronic) IS - 2692-9368 (Linking) VI - 18 IP - 6 DP - 2026 Nov-Dec TI - Artificial Intelligence-Driven Graph Methods for Microbiome Research: A Systematic Review. LID - e70016 [pii] LID - 10.1002/wsbm.70016 [doi] AB - The complexity of high-throughput sequencing data in microbiome research necessitates advanced analytical methods to decipher microbial community structures and interactions. Graph-based methods, which model taxa as nodes and their relationships as edges, have emerged as a powerful framework for this purpose. However, the rapid evolution of these techniques, from classical network analysis to modern graph representation learning, has created a fragmented and complex methodological landscape. This systematic review provides a comprehensive synthesis and structured overview of the field. We systematically review the literature and propose a three-part classification to categorize the primary contribution of each study: (1) novel methods for microbial network representation, (2) applications of classical graph analysis, and (3) the emerging use of graph representation learning for predictive modeling. We summarize the most frequent applications within each class, including keystone species identification, community clustering, and graph-based disease classification. Furthermore, we identify critical cross-cutting challenges, such as the lack of standardized tools and the need for improved model interpretability. By structuring the current landscape of graph-based methods, this review serves as a crucial guide for researchers to select appropriate models and highlights key gaps to direct future research. CI - (c) 2026 The Author(s). WIREs Mechanisms of Disease published by Wiley Periodicals LLC. FAU - Shum-Tim, Cedrique AU - Shum-Tim C AD - Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine and Health Sciences, McGill University, Quebec, Canada. AD - Computational Biology and Artificial Intelligence Laboratory, Department of Electrical and Computer Engineering, Faculty of Engineering, McGill University, Quebec, Canada. AD - Mila - Quebec AI Institute, McGill University, Quebec, Canada. FAU - Islam, Paromita AU - Islam P AUID- ORCID: 0000-0001-6295-2611 AD - Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine and Health Sciences, McGill University, Quebec, Canada. FAU - Boyajian, Jacqueline L AU - Boyajian JL AUID- ORCID: 0009-0009-9054-2463 AD - Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine and Health Sciences, McGill University, Quebec, Canada. FAU - Abosalha, Ahmed AU - Abosalha A AD - Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine and Health Sciences, McGill University, Quebec, Canada. AD - Pharmaceutical Technology Department, Faculty of Pharmacy, Tanta University, Tanta, Egypt. FAU - Kassab, Amal AU - Kassab A AUID- ORCID: 0000-0002-8371-8370 AD - Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine and Health Sciences, McGill University, Quebec, Canada. FAU - Santos, Madison AU - Santos M AD - Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine and Health Sciences, McGill University, Quebec, Canada. FAU - Khan, Kinaan Aamir AU - Khan KA AUID- ORCID: 0009-0007-9015-0289 AD - Computational Biology and Artificial Intelligence Laboratory, Department of Electrical and Computer Engineering, Faculty of Engineering, McGill University, Quebec, Canada. AD - Mila - Quebec AI Institute, McGill University, Quebec, Canada. FAU - Stern, Kiri AU - Stern K AD - Computational Biology and Artificial Intelligence Laboratory, Department of Electrical and Computer Engineering, Faculty of Engineering, McGill University, Quebec, Canada. AD - Mila - Quebec AI Institute, McGill University, Quebec, Canada. AD - Department of Quantitative Life Sciences, Faculty of Interfaculty Studies, McGill University, Quebec, Canada. FAU - Emad, Amin AU - Emad A AUID- ORCID: 0000-0002-5108-4887 AD - Computational Biology and Artificial Intelligence Laboratory, Department of Electrical and Computer Engineering, Faculty of Engineering, McGill University, Quebec, Canada. AD - Mila - Quebec AI Institute, McGill University, Quebec, Canada. AD - Department of Quantitative Life Sciences, Faculty of Interfaculty Studies, McGill University, Quebec, Canada. FAU - Prakash, Satya AU - Prakash S AUID- ORCID: 0000-0002-4902-2353 AD - Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine and Health Sciences, McGill University, Quebec, Canada. LA - eng GR - 252743/Canadian Institute of Health Research/ GR - 335999/Fonds de Recherche du Quebec-Sante/ GR - 351337/Fonds de Recherche du Quebec-Sante/ GR - 2020-245622/Islamic Development Bank Scholarship/ GR - Ministry of Higher Education of the Arab Republic of Egypt/ GR - 504933/Natural Sciences and Engineering Research Council of Canada (NSERC)/ PT - Journal Article PT - Review PT - Systematic Review PL - United States TA - WIREs Mech Dis JT - WIREs mechanisms of disease JID - 9918227353306676 SB - IM MH - *Microbiota/genetics MH - *Artificial Intelligence MH - Humans MH - Animals OTO - NOTNLM OT - deep learning OT - graph representation learning OT - high-throughput sequencing OT - metagenomics OT - microbiome OT - network science OT - networks EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:29 PHST- 2026/07/15 00:00 [revised] PHST- 2026/05/25 00:00 [received] PHST- 2026/07/29 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:29 [entrez] AID - e70016 [pii] AID - 10.1002/wsbm.70016 [doi] PST - ppublish SO - WIREs Mech Dis. 2026 Nov-Dec;18(6):e70016. doi: 10.1002/wsbm.70016. PMID- 42953984 OWN - NLM STAT- Publisher LR - 20261029 IS - 1746-0921 (Electronic) IS - 1746-0913 (Linking) DP - 2026 Oct 29 TI - Natural and engineered bacteriophages against multidrug-resistant ESKAPE pathogens: current landscape and clinical translation. PG - 1-15 LID - 10.1080/17460913.2026.2752147 [doi] AB - The worldwide emergence of antimicrobial resistance and the need for non-conventional alternatives to conventional antibiotics have been increased by multidrug-resistant ESKAPE pathogens, which cause the majority of nosocomial infections and are starting to develop resistance to even the most potent drugs. Engineered bacteriophages are promising precision antimicrobials because of their ability to selectively infect and lyse bacterial hosts, multiply at infection sites, and co-evolve with bacterial populations to overcome resistance. Advances in synthetic biology, such as tail fiber modification, depolymerase and endolysin enhancement, genome editing using the clustered regularly interspaced short palindromic repeats (CRISPR), and computationally designed phage cocktails, have opened new possibilities for therapeutics that were hindered by the limitations of natural phages. These engineering strategies expand host range, enhance biofilm penetration, and target resistance genes. In spite of promising evidence in preclinical models, the use of engineered phages in routine clinical practice remains difficult. Specific key challenges include unclear regulations, lack of good manufacturing practice (GMP)grade production, variable pharmacokinetics and limited clinical trial data. This review critically examines engineering approaches to improve phage activity against ESKAPE pathogens, identifies the scientific and regulatory barriers limiting clinical use, and outlines the key steps needed for wider adoption. FAU - Nautiyal, Deeksha AU - Nautiyal D AUID- ORCID: 0009-0001-1849-7620 AD - School of Health Sciences and Technology, UPES, Dehradun, India. FAU - Sahu, Mridul AU - Sahu M AUID- ORCID: 0009-0006-6035-3416 AD - School of Health Sciences and Technology, UPES, Dehradun, India. FAU - Jain, Utkarsh AU - Jain U AUID- ORCID: 0000-0003-3901-0153 AD - School of Health Sciences and Technology, UPES, Dehradun, India. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - England TA - Future Microbiol JT - Future microbiology JID - 101278120 SB - IM OTO - NOTNLM OT - Antimicrobial resistance OT - ESKAPE pathogens OT - engineered bacteriophages OT - multidrug-resistant bacteria OT - phage therapy EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:29 PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:29 [entrez] AID - 10.1080/17460913.2026.2752147 [doi] PST - aheadofprint SO - Future Microbiol. 2026 Oct 29:1-15. doi: 10.1080/17460913.2026.2752147. PMID- 42953980 OWN - NLM STAT- Publisher LR - 20261029 IS - 1460-2156 (Electronic) IS - 0006-8950 (Linking) DP - 2026 Oct 29 TI - Cerebellar subregions support social cognition in early behavioural variant frontotemporal dementia. LID - awag381 [pii] LID - 10.1093/brain/awag381 [doi] AB - Social cognitive deficits manifest early in behavioral variant frontotemporal dementia (bvFTD), compromising individuals' ability to interpret social cues, empathize, and maintain interpersonal relationships. Current neurobiological models emphasize cortical network disruption, particularly within the salience, semantic appraisal, and default mode networks, as primary contributors to social dysfunction. However, despite the increasingly recognized role of the cerebellum in higher-order cognitive and socioemotional processes, its contributions to social deficits remains underexplored in bvFTD. Here, we investigated the unique and network-level influences of cerebellar subregions on social cognition across experimental and real-world assessments in early-stage bvFTD. A total of 107 individuals with bvFTD and 100 healthy controls were evaluated across three social domains: theory of mind (ToM) (The Awareness of Social Inference Test-Social Inference-Enriched, TASIT-SIE; Cognitive Emotional Perspective Taking Test), emotion reading (Dynamic Affect Recognition Test; TASIT-Emotion Evaluation Test; Comprehensive Affect Testing System), and real-life empathy (Revised Self-Monitoring Scale; Interpersonal Reactivity Index). Using a functional cerebellar parcellation, we extracted volumes of posterior cerebellar subregions implicated in social-linguistic and multiple-demand networks from participants' structural MRIs. To address the high collinearity between cerebellar and cortical atrophy patterns, we applied sequential regression, partial least squares correlation, and dominance analysis to characterize brain-behavior relationships. Additionally, supplementary structural equation modelling (SEM) examined whether the associations between cerebellar integrity and social cognition was mediated by executive functioning. Our results showed that cerebellar subregional integrity contributed to social cognitive deficits in individuals with early-stage bvFTD. Cerebellar subregions, particularly right Crus I and II, showed the strongest and most selective contribution to high-level inferential Theory of Mind performance. Tasks involving dynamic emotion stimuli exhibited a more distributed pattern of cerebello-cerebral involvement. By contrast, questionnaire-based measures of real-world social functioning were less prominently represented in the latent covariance structure. SEM further showed that cerebellar integrity remained directly associated with social cognition after accounting for executive functioning, whereas the indirect association through executive functioning was not significant. These findings highlight the task-dependent nature of cerebellar involvement in social cognition, and demonstrate that time-limited experimental tasks, particularly those involving inferential reasoning about beliefs and affect, are likely to be especially valuable in detecting subtle cerebello-cerebral network dysfunction that questionnaire-based measures may overlook. Our results extend cortex-centric models of social dysfunction in bvFTD by demonstrating that cerebellar subregions contribute to distributed cerebello-cerebral systems associated with social cognitive performance. These findings provide a foundation for future longitudinal and mechanistic studies of cerebello-cerebral dysfunction in neurodegenerative disease. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Guarantors of Brain. FAU - Chen, Yu AU - Chen Y AUID- ORCID: 0000-0001-5863-7002 AD - Department of Neurology, Weill Institute for Neurosciences, Edward and Pearl Fein Memory and Aging Center, University of California San Francisco, San Francisco, CA, 94158, USA. FAU - Chatzidimitriou, Electra AU - Chatzidimitriou E AD - Department of Neurology, Weill Institute for Neurosciences, Edward and Pearl Fein Memory and Aging Center, University of California San Francisco, San Francisco, CA, 94158, USA. AD - Department of Cognition, Brain and Behavior, School of Psychology, Faculty of Philosophy, Aristotle University of Thessaloniki, Thessaloniki, Greece. FAU - Yang, Winson Fu Zun AU - Yang WFZ AD - Department of Psychiatry, Meditation Research Program, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02129, USA. AD - Department of Radiology, Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USA. FAU - Leng, Fangda AU - Leng F AD - Department of Neurology, Weill Institute for Neurosciences, Edward and Pearl Fein Memory and Aging Center, University of California San Francisco, San Francisco, CA, 94158, USA. FAU - Syed, Faatimah AU - Syed F AD - Department of Neurology, Weill Institute for Neurosciences, Edward and Pearl Fein Memory and Aging Center, University of California San Francisco, San Francisco, CA, 94158, USA. FAU - McEachen, Bailey AU - McEachen B AD - Department of Neurology, Weill Institute for Neurosciences, Edward and Pearl Fein Memory and Aging Center, University of California San Francisco, San Francisco, CA, 94158, USA. FAU - Ivry, Richard B AU - Ivry RB AD - Department of Psychology, University of California Berkeley, Berkeley, CA, 94720, USA. AD - Helen Wills Neuroscience Institute, University of California Berkeley, Berkeley, CA, 94720, USA. FAU - Rosen, Howard J AU - Rosen HJ AD - Department of Neurology, Weill Institute for Neurosciences, Edward and Pearl Fein Memory and Aging Center, University of California San Francisco, San Francisco, CA, 94158, USA. FAU - Seeley, William W AU - Seeley WW AD - Department of Neurology, Weill Institute for Neurosciences, Edward and Pearl Fein Memory and Aging Center, University of California San Francisco, San Francisco, CA, 94158, USA. AD - Department of Pathology, University of California San Francisco, San Francisco, CA, 94158, USA. FAU - Gorno-Tempini, Maria Luisa AU - Gorno-Tempini ML AD - Department of Neurology, Weill Institute for Neurosciences, Edward and Pearl Fein Memory and Aging Center, University of California San Francisco, San Francisco, CA, 94158, USA. AD - Dyslexia Center, University of California San Francisco, San Francisco, CA, 94158, USA. FAU - Kramer, Joel H AU - Kramer JH AD - Department of Neurology, Weill Institute for Neurosciences, Edward and Pearl Fein Memory and Aging Center, University of California San Francisco, San Francisco, CA, 94158, USA. FAU - Miller, Bruce L AU - Miller BL AUID- ORCID: 0000-0002-2152-4220 AD - Department of Neurology, Weill Institute for Neurosciences, Edward and Pearl Fein Memory and Aging Center, University of California San Francisco, San Francisco, CA, 94158, USA. FAU - Rankin, Katherine P AU - Rankin KP AD - Department of Neurology, Weill Institute for Neurosciences, Edward and Pearl Fein Memory and Aging Center, University of California San Francisco, San Francisco, CA, 94158, USA. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Brain JT - Brain : a journal of neurology JID - 0372537 SB - IM OTO - NOTNLM OT - behavioral variant frontotemporal dementia OT - brain-behavior relationships OT - cerebello-cortical networks OT - cerebellum OT - multivariate analysis OT - social cognition EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:29 PHST- 2026/03/06 00:00 [received] PHST- 2026/09/10 00:00 [revised] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:29 [entrez] AID - 9004307 [pii] AID - 10.1093/brain/awag381 [doi] PST - aheadofprint SO - Brain. 2026 Oct 29:awag381. doi: 10.1093/brain/awag381. PMID- 42953979 OWN - NLM STAT- Publisher LR - 20261029 IS - 1539-2570 (Electronic) IS - 0271-6798 (Linking) DP - 2026 Oct 27 TI - Radiologic Results of Selective Thoracic Fusion in Adolescent Idiopathic Scoliosis (AIS) Lenke Type 3 and 4. LID - 10.1097/BPO.0000000000003507 [doi] AB - BACKGROUND: Selective thoracic fusion (STF) in Lenke type 3 and 4 adolescent idiopathic scoliosis (AIS) remains controversial because the thoracolumbar/lumbar curve is structural and is traditionally included in the fusion. This study evaluated the early radiographic outcomes of STF in preference-selected patients with Lenke type 3 and 4 AIS. METHODS: A retrospective review was performed of 22 patients with Lenke type 3 or 4 AIS who underwent posterior STF between 2016 and 2022. Standing radiographs were evaluated preoperatively, immediately postoperatively, and at 1-year follow-up. Thoracic and lumbar curve magnitude, coronal and sagittal balance, lumbar lordosis, and radiographic factors potentially associated with spontaneous lumbar correction were analyzed. RESULTS: Seventeen patients had Lenke type 3, and 5 had Lenke type 4 curves. The thoracic curve improved from 68 degrees preoperatively to 28 degrees immediately postoperatively (P<0.001). The unfused lumbar curve improved from 49 degrees preoperatively to 29 degrees immediately postoperatively and measured 30 degrees at 1-year follow-up (P<0.001 compared with preoperative values). Coronal balance worsened transiently immediately after surgery but returned to approximately the preoperative position at 1 year. Lumbar lordosis decreased from 56 degrees preoperatively to 48 degrees immediately postoperatively and recovered to 60 degrees at 1 year. Greater thoracic correction was associated with greater immediate lumbar correction (Spearman rho=0.521, P=0.014), but this association was not maintained at 1 year. Preoperative lumbar Cobb angle (P=0.082) demonstrated borderline significance as a predictor; otherwise, no significant predictors were identified. One patient (5%) required revision with distal extension of the fusion to L3. CONCLUSIONS: In this preference-selected cohort of patients with Lenke type 3 and 4 AIS, STF was associated with spontaneous improvement of the unfused structural lumbar curve during the first postoperative year. Early postoperative coronal imbalance and loss of lumbar lordosis largely recovered by 1 year. STF may be considered in carefully counseled patients seeking preservation of lumbar motion segments; however, these findings do not establish general selection criteria or long-term durability. LEVELS OF EVIDENCE: Level IV. CI - Copyright (c) 2026 Wolters Kluwer Health, LLC. All rights reserved. FAU - Alkharsawi, Mahmoud AU - Alkharsawi M AUID- ORCID: 0000-0002-5492-9419 AD - Eifelklinik St. Brigida, Simmerath. FAU - Trobisch, Per David AU - Trobisch PD AD - Eifelklinik St. Brigida, Simmerath. FAU - Frank, Jil AU - Frank J AD - Department of Orthopaedic Trauma and Reconstructive Surgery, University Hospital RWTH, Aachen, Germany. FAU - Pishnamaz MeD, Miguel AU - Pishnamaz MeD M AD - Department of Orthopaedic Trauma and Reconstructive Surgery, University Hospital RWTH, Aachen, Germany. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Pediatr Orthop JT - Journal of pediatric orthopedics JID - 8109053 SB - IM OTO - NOTNLM OT - Lenke 3 and 4 OT - scoliosis OT - selective thoracic fusion COIS- P.T. reported relationships with Globus Medical in the form of paid lectures and research grants, and with Medtronic as a consultant. The remaining authors declare no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:29 PHST- 2026/06/29 00:00 [received] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:29 [entrez] AID - 01241398-990000000-01352 [pii] AID - 10.1097/BPO.0000000000003507 [doi] PST - aheadofprint SO - J Pediatr Orthop. 2026 Oct 27. doi: 10.1097/BPO.0000000000003507. PMID- 42953971 OWN - NLM STAT- Publisher LR - 20261029 IS - 1460-2156 (Electronic) IS - 0006-8950 (Linking) DP - 2026 Oct 29 TI - E3 ubiquitin ligases: a vital component of proteostasis impaired in hereditary neuropathies. LID - awag371 [pii] LID - 10.1093/brain/awag371 [doi] AB - Protein homeostasis is essential for the maintenance of peripheral axons and depends on co-ordinated protein synthesis, folding, ubiquitination and directing protein fate across multiple protein quality-control pathways. E3 ubiquitin ligases are central to this network by conferring substrate specificity and enabling precise spatiotemporal regulation of protein homeostasis. Increasing genetic and functional evidence implicates the disruption of E3 ligase function in inherited peripheral neuropathies, providing a mechanistic link between impaired proteostasis and progressive axonal degeneration. Here, we examine inherited peripheral neuropathies associated with dysfunction of Tripartite Motif-Containing Protein 2 (TRIM2), Leucine Rich Repeat and Sterile Alpha Motif Containing 1 (LRSAM1), Ubiquitin Protein Ligase E3C (UBE3C) and the Cullin-Ring 3 (CUL3) substrate adapter, Gigaxonin (GAN). Although these proteins differ in structure, substrates and disease mechanisms, their dysfunction converges on disruption of proteasomal degradation, autophagy and cytoskeletal homeostasis. By integrating these genetically distinct neuropathies within a common mechanistic framework, we propose this positions E3 ubiquitin ligases as selective regulators of multiple protein quality-control pathways and highlights the specific vulnerability of long peripheral axons to the disruption of ubiquitin-mediated proteostasis. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Guarantors of Brain. FAU - Gonebale, Madison D V AU - Gonebale MDV AUID- ORCID: 0009-0005-2874-8306 AD - Northcott Neuroscience Laboratory, ANZAC Research Institute, Sydney, NSW 2139, Australia. AD - Faculty of Medicine and Health, University of Sydney, Sydney, NSW 2006, Australia. FAU - Cutrupi, Anthony N AU - Cutrupi AN AUID- ORCID: 0000-0002-4527-3710 AD - Northcott Neuroscience Laboratory, ANZAC Research Institute, Sydney, NSW 2139, Australia. AD - Faculty of Medicine and Health, University of Sydney, Sydney, NSW 2006, Australia. FAU - Kennerson, Marina L AU - Kennerson ML AUID- ORCID: 0000-0003-3332-5074 AD - Northcott Neuroscience Laboratory, ANZAC Research Institute, Sydney, NSW 2139, Australia. AD - Faculty of Medicine and Health, University of Sydney, Sydney, NSW 2006, Australia. AD - Molecular Medicine Laboratory, Concord Repatriation General Hospital, Sydney, NSW 2139, Australia. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Brain JT - Brain : a journal of neurology JID - 0372537 SB - IM OTO - NOTNLM OT - autophagy OT - inherited peripheral neuropathies OT - ubiquitin proteasome system; E3 ubiquitin ligases EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:28 PHST- 2026/05/07 00:00 [received] PHST- 2026/09/01 00:00 [revised] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:28 [entrez] AID - 9004302 [pii] AID - 10.1093/brain/awag371 [doi] PST - aheadofprint SO - Brain. 2026 Oct 29:awag371. doi: 10.1093/brain/awag371. PMID- 42953969 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2561-326X (Electronic) IS - 2561-326X (Linking) VI - 10 DP - 2026 Oct 22 TI - Effectiveness and Safety of Tasso+ Pediatric Microsampling for Child Blood Lead Levels: Cohort Study. PG - e101700 LID - 10.2196/101700 [doi] AB - BACKGROUND: The growing interest in promoting child health requires minimally invasive biospecimen collection. Particularly during and since the COVID-19 pandemic, there has been increased interest in microsampling technology to democratize participation in research involving biospecimens, particularly for children. These advances mitigate barriers, such as scheduling, transportation, and psychosocial challenges. Tasso is a microsampling biomedical device that has gained popularity in research. It uses a vacuum-assisted capillary system that attaches to the upper arm and is considered minimally invasive, as it requires no venipuncture or phlebotomist. Yet, important gaps remain in understanding Tasso performance, particularly among pediatric populations where its administration is limited. OBJECTIVE: This research addresses a significant gap in biomedical device development by examining the effectiveness, safety, and failure rate of the Tasso+ Class II medical device designed for blood sampling (puncture) among children. The Tasso+ was selected for a pediatric cohort research study given advertisement of methodological advantages over traditional phlebotomy. This original investigation characterizes the effectiveness of and potential for institutional review board-designated adverse events, following the use of the Tasso+ Class II medical device within a pediatric population. METHODS: Blood collection was essential to measure lead (Pb) exposures for children residing in a region known to be polluted. Parents/guardians consented, and children assented to Tasso+ sampling on the upper arm. The Tasso+ device was administered in 2024 by trained researchers on 2 pilot participants (ages 7-8 years) and subsequently 13 study participants (ages 7-10 years). Sampling was terminated upon participant reports of persistent pain and scarring on children's arms. Follow-up was conducted at intervals after device application: 2-11 weeks and 10-19 months. Data sources included data collection and laboratory records and survey-based follow-up with participating families. Analyses involved descriptive statistics drawn from data collection and laboratory records and descriptive statistics and content analysis of a follow-up survey administered to participating families. Content analysis of open-ended responses to the follow-up survey administered to families identified themes and their frequency of occurrence. Main measures included the device failure rate, effectiveness (ie, blood volume), and adverse outcomes at the arm puncture site. RESULTS: The device failure rate was 26.7% (4/15), with only 66.7% (10/15) and 20% (3/20) of the cases having the optimal (>450 microL) or adequate (250-449 microL) blood volume, respectively. At initial follow-up, 23.1% reported ongoing pain; none reported ongoing pain at extended follow-up. Scarring showed high initial incidence (61.5%) and persistence (75.0%). Children reported shame related to the participant-reported and researcher-observed scar and impacts of pain on feelings toward physical touch (eg, hug/embrace). CONCLUSIONS: These effectiveness barriers and adverse events highlight the need for documentation to inform the future rollout of novel devices for participatory research, longer-term follow-up, and addressing potential gaps in transparency between manufacturers and researchers who use biomedical devices. CI - (c) Alana M W LeBron, Kristina Uban, Julia Mangione, Olaya Daki, Erika Ramirez-Mayoral, Maya Cheav, Sarahi Gutierrez Luna, Adriana Orellana, Yohana Rojas de Jesus, Yezenia Marrujo, Blanca I Contreras, Teresa Campos, Jun Wu. Originally published in JMIR Formative Research (https://formative.jmir.org). FAU - LeBron, Alana M W AU - LeBron AMW AUID- ORCID: 0000-0003-0964-4673 AD - Department of Health, Society, and Behavior, Joe C. Wen School of Population & Public Health, University of California, Irvine, 856 Health Sciences Quad, Office 3555, Irvine, CA, 92697, United States, 1 949-824-5242, 1 949-824-1019. AD - Department of Chicano/Latino Studies, School of Social Sciences, University of California, Irvine, Irvine, CA, United States. FAU - Uban, Kristina AU - Uban K AUID- ORCID: 0000-0002-2727-0815 AD - Department of Health, Society, and Behavior, Joe C. Wen School of Population & Public Health, University of California, Irvine, 856 Health Sciences Quad, Office 3555, Irvine, CA, 92697, United States, 1 949-824-5242, 1 949-824-1019. AD - Institute of Interdisciplinary Salivary Bioscience Research, University of California, Irvine, Irvine, CA, United States. AD - Center for the Neurobiology of Learning and Memory (CNLM), University of California, Irvine, Irvine, CA, United States. FAU - Mangione, Julia AU - Mangione J AUID- ORCID: 0009-0000-2859-3723 AD - Department of Health, Society, and Behavior, Joe C. Wen School of Population & Public Health, University of California, Irvine, 856 Health Sciences Quad, Office 3555, Irvine, CA, 92697, United States, 1 949-824-5242, 1 949-824-1019. FAU - Daki, Olaya AU - Daki O AUID- ORCID: 0009-0007-2130-6454 AD - Department of Health, Society, and Behavior, Joe C. Wen School of Population & Public Health, University of California, Irvine, 856 Health Sciences Quad, Office 3555, Irvine, CA, 92697, United States, 1 949-824-5242, 1 949-824-1019. FAU - Ramirez-Mayoral, Erika AU - Ramirez-Mayoral E AUID- ORCID: 0009-0009-0291-8441 AD - Community Member At-Large, Santa Ana, CA, United States. FAU - Cheav, Maya AU - Cheav M AUID- ORCID: 0009-0001-6680-9561 AD - Orange County Environmental Justice, Santa Ana, CA, United States. FAU - Gutierrez Luna, Sarahi AU - Gutierrez Luna S AUID- ORCID: 0009-0004-8611-091X AD - Community Member At-Large, Santa Ana, CA, United States. FAU - Orellana, Adriana AU - Orellana A AUID- ORCID: 0009-0007-8801-3189 AD - Community Member At-Large, Santa Ana, CA, United States. FAU - Rojas de Jesus, Yohana AU - Rojas de Jesus Y AUID- ORCID: 0009-0009-8501-3014 AD - Community Member At-Large, Santa Ana, CA, United States. FAU - Marrujo, Yezenia AU - Marrujo Y AUID- ORCID: 0009-0001-1917-6642 AD - Community Member At-Large, Santa Ana, CA, United States. FAU - Contreras, Blanca I AU - Contreras BI AUID- ORCID: 0009-0007-5826-7153 AD - Community Member At-Large, Santa Ana, CA, United States. FAU - Campos, Teresa AU - Campos T AUID- ORCID: 0009-0007-0909-2107 AD - Community Member At-Large, Santa Ana, CA, United States. FAU - Wu, Jun AU - Wu J AUID- ORCID: 0000-0002-2693-7112 AD - Department of Environmental and Occupational Health, Joe C. Wen School of Population & Public Health, University of California, Irvine, Irvine, CA, United States. LA - eng PT - Journal Article DEP - 20261022 PL - Canada TA - JMIR Form Res JT - JMIR formative research JID - 101726394 RN - 2P299V784P (Lead) SB - IM MH - Humans MH - Child MH - *Blood Specimen Collection/instrumentation/methods/adverse effects MH - *Lead/blood MH - Cohort Studies MH - Male MH - Female MH - Phlebotomy/instrumentation MH - COVID-19/epidemiology OTO - NOTNLM OT - Tasso OT - adverse events OT - biosampling OT - child blood lead levels OT - clinical research OT - environmental health OT - epidemiology OT - methodology OT - pediatric research EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:28 PHST- 2026/05/19 00:00 [received] PHST- 2026/09/08 00:00 [revised] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:28 [entrez] AID - v10i1e101700 [pii] AID - 10.2196/101700 [doi] PST - epublish SO - JMIR Form Res. 2026 Oct 22;10:e101700. doi: 10.2196/101700. PMID- 42953968 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1362-4962 (Electronic) IS - 0305-1048 (Linking) VI - 54 IP - 20 DP - 2026 Oct 24 TI - Metal-ion coordination drives active-site remodeling for backsplicing in circularly permuted group II introns. LID - gkag1053 [pii] LID - 10.1093/nar/gkag1053 [doi] AB - Circularly permuted (CP) group II introns are self-splicing ribozymes that undergo a sequential branching and backsplicing reaction to yield branched introns and circular exons (circRNAs). While the initial branching step is structurally characterized, the mechanistic principles that govern the subsequent large-scale active-site remodeling and stringent metal-ion specificity required for backsplicing remain poorly understood. Here, we present a series of high-resolution cryo-EM structures of the CP group II intron from Gluconacetobacter diazotrophicus PAL5 (Gdi-1), capturing the ribozyme at discrete stages along the branching -to- backsplicing pathway under distinct ionic conditions. Comparative analyses under K+/Mg2+, K+/Ca2+, and Cs+/Mg2+ conditions demonstrate that productive backsplicing relies on ion-specific coordination networks that govern 3' splice site (3'SS) docking and catalytic center formation, revealing the precise metal-ion-coordinated conformational rearrangements and dynamic splice-site exchange mechanisms. Crucially, we identify a previously unrecognized, highly selective monovalent cation-binding site, K3, that structurally couples the gamma-gamma' interaction to reassembly of the heteronuclear four-metal core, thereby promoting productive second-step active-site formation. Together, these findings establish a structural basis for metal-ion-dependent active-site remodeling during group II intron catalysis and provide mechanistic insights into metal-ion selectivity in circRNA biogenesis. CI - (c) The Author(s) 2026. Published by Oxford University Press. FAU - Zhang, Jie AU - Zhang J AUID- ORCID: 0009-0004-8506-531X AD - State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, School of Life Sciences, Inner Mongolia University, Hohhot 010020, China. AD - State Key Laboratory of Genetics and Development of Complex Phenotypes, Department of Biochemistry and Biophysics, School of Life Sciences, Fudan University, Shanghai 200438, China. FAU - Yao, Yuqi AU - Yao Y AUID- ORCID: 0009-0000-7232-4926 AD - State Key Laboratory of Genetics and Development of Complex Phenotypes, Department of Biochemistry and Biophysics, School of Life Sciences, Fudan University, Shanghai 200438, China. FAU - Ma, Jinbiao AU - Ma J AUID- ORCID: 0000-0002-0232-1786 AD - State Key Laboratory of Genetics and Development of Complex Phenotypes, Department of Biochemistry and Biophysics, School of Life Sciences, Fudan University, Shanghai 200438, China. AD - Center for mRNA Translational Research, Fudan University, Shanghai 200438, China. LA - eng GR - 32471347/National Natural Science Foundation of China/ GR - 23XD1400300/Program of Shanghai Academic/Technology Research Leader/ PT - Journal Article PL - England TA - Nucleic Acids Res JT - Nucleic acids research JID - 0411011 RN - 0 (RNA, Catalytic) RN - 0 (Metals) RN - 0 (RNA Splice Sites) SB - IM MH - *Introns/genetics MH - *RNA, Catalytic/chemistry/metabolism/genetics MH - Catalytic Domain MH - RNA Splicing MH - *Metals/chemistry/metabolism MH - Cryoelectron Microscopy MH - Models, Molecular MH - Nucleic Acid Conformation MH - RNA Splice Sites MH - Binding Sites EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:28 PHST- 2026/07/10 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:28 [entrez] AID - 9004261 [pii] AID - 10.1093/nar/gkag1053 [doi] PST - ppublish SO - Nucleic Acids Res. 2026 Oct 24;54(20):gkag1053. doi: 10.1093/nar/gkag1053. PMID- 42953963 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1362-4962 (Electronic) IS - 0305-1048 (Linking) VI - 54 IP - 20 DP - 2026 Oct 24 TI - EWSR1 associates with the 40S ribosomal subunit and represses translation to maintain endoplasmic reticulum homeostasis. LID - gkag1022 [pii] LID - 10.1093/nar/gkag1022 [doi] AB - FET family proteins (FUS, EWSR1, and TAF15) have garnered significant attention due to their involvement in oncogenic fusions and mutations in various cancers and neurodegenerative disorders. Although these highly conserved RNA-binding proteins are widely described as multifunctional regulators of gene expression, their precise molecular functions remain incompletely understood. Here, we uncover a previously unrecognized role for EWSR1 as a selective translational repressor in cancer cells. EWSR1 specifically targets mRNAs involved in lipid metabolism and endoplasmic reticulum (ER) homeostasis. Loss of EWSR1 disrupts ER morphology and impairs activation of the unfolded protein response of the ER (UPRER). Mechanistically, EWSR1 represses translation upon association with target mRNAs, and its conserved region spanning two arginine-glycine-glycine (RGG) boxes contributes to association with the 40S ribosomal subunit. These findings define a novel role for EWSR1 in post-transcriptional gene regulation and suggest that perturbations in its translational activity may underlie the pathogenesis of FET-associated diseases. CI - (c) The Author(s) 2026. Published by Oxford University Press. FAU - Hassoun, Zahrat El Oula AU - Hassoun ZEO AD - Laboratory of Gene Expression and Cancer, GIGA Institute, University of Liege, 4000 Liege, Belgium. FAU - Ongena, Loic AU - Ongena L AD - Laboratory of Gene Expression and Cancer, GIGA Institute, University of Liege, 4000 Liege, Belgium. FAU - Conde, Lionel AU - Conde L AD - CIRI, Centre International de Recherche en Infectiologie, Univ Lyon, INSERM U1111, Universite Claude Bernard Lyon 1, CNRS UMR5308, ENS de Lyon, F-69007 Lyon, France. FAU - Galvan, Bartimee AU - Galvan B AUID- ORCID: 0000-0001-8629-4860 AD - Laboratory of Gene Expression and Cancer, GIGA Institute, University of Liege, 4000 Liege, Belgium. FAU - Fettweis, Gregory AU - Fettweis G AUID- ORCID: 0000-0002-6623-2264 AD - Laboratory of Gene Expression and Cancer, GIGA Institute, University of Liege, 4000 Liege, Belgium. FAU - Lion, Michelle AU - Lion M AD - Laboratory of Gene Expression and Cancer, GIGA Institute, University of Liege, 4000 Liege, Belgium. FAU - Soin, Romuald AU - Soin R AD - Laboratoire de Biologie Moleculaire du Gene, Institut de Biologie et de Medecine Moleculaires, Universite Libre de Bruxelles (ULB), 6041 Gosselies, Belgium. FAU - Bruyr, Jonathan AU - Bruyr J AD - Laboratory of Gene Expression and Cancer, GIGA Institute, University of Liege, 4000 Liege, Belgium. FAU - Lemaitre, Florence AU - Lemaitre F AD - Laboratory of Gene Expression and Cancer, GIGA Institute, University of Liege, 4000 Liege, Belgium. FAU - Thiry, Marc AU - Thiry M AD - Laboratory of Cell and Tissue Biology, GIGA Institute, University of Liege, 4000 Liege, Belgium. FAU - Mariavelle, Emeline AU - Mariavelle E AD - Laboratory of Gene Expression and Cancer, GIGA Institute, University of Liege, 4000 Liege, Belgium. FAU - Claes, Margaux AU - Claes M AD - Laboratory of Gene Expression and Cancer, GIGA Institute, University of Liege, 4000 Liege, Belgium. FAU - Blomme, Arnaud AU - Blomme A AD - Laboratory of Metabolic Regulation, GIGA Institute, University of Liege, 4000 Liege, Belgium. FAU - Menschaert, Gerben AU - Menschaert G AD - Biobix, Laboratory of Bioinformatics and Computational Genomics, Ghent University, 9000 Ghent, Belgium. FAU - Dehairs, Jonas AU - Dehairs J AD - Laboratory of Lipid Metabolism and Cancer, Department of Oncology, Leuven Cancer Institute and Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven B-3000, Belgium. FAU - Kruys, Veronique AU - Kruys V AD - Laboratoire de Biologie Moleculaire du Gene, Institut de Biologie et de Medecine Moleculaires, Universite Libre de Bruxelles (ULB), 6041 Gosselies, Belgium. FAU - Gueydan, Cyril AU - Gueydan C AUID- ORCID: 0000-0003-3097-7667 AD - Laboratoire de Biologie Moleculaire du Gene, Institut de Biologie et de Medecine Moleculaires, Universite Libre de Bruxelles (ULB), 6041 Gosselies, Belgium. FAU - O'grady, Tina M AU - O'grady TM AUID- ORCID: 0000-0002-1283-0293 AD - Laboratory of Gene Expression and Cancer, GIGA Institute, University of Liege, 4000 Liege, Belgium. FAU - Swinnen, Johannes V AU - Swinnen JV AD - Laboratory of Lipid Metabolism and Cancer, Department of Oncology, Leuven Cancer Institute and Leuven Institute for Single Cell Omics (LISCO), KU Leuven, Leuven B-3000, Belgium. FAU - de Breyne, Sylvain AU - de Breyne S AUID- ORCID: 0000-0002-1632-3475 AD - CIRI, Centre International de Recherche en Infectiologie, Univ Lyon, INSERM U1111, Universite Claude Bernard Lyon 1, CNRS UMR5308, ENS de Lyon, F-69007 Lyon, France. FAU - Ohlmann, Theophile AU - Ohlmann T AD - CIRI, Centre International de Recherche en Infectiologie, Univ Lyon, INSERM U1111, Universite Claude Bernard Lyon 1, CNRS UMR5308, ENS de Lyon, F-69007 Lyon, France. FAU - Twizere, Jean-Claude AU - Twizere JC AUID- ORCID: 0000-0002-8683-705X AD - Laboratory of Viral Interactomes Networks, GIGA Institute, University of Liege, 4000 Liege, Belgium. FAU - Habraken, Yvette AU - Habraken Y AUID- ORCID: 0000-0003-1692-3822 AD - Laboratory of Gene Expression and Cancer, GIGA Institute, University of Liege, 4000 Liege, Belgium. FAU - Dequiedt, Franck AU - Dequiedt F AUID- ORCID: 0000-0003-1234-7477 AD - Laboratory of Gene Expression and Cancer, GIGA Institute, University of Liege, 4000 Liege, Belgium. LA - eng GR - Belgian National Fund for Scientific Research/ GR - Universite de Liege/ GR - GIGA Doctoral School for Health Sciences/ GR - FSR-FNRS/ GR - Dequiedt laboratory/ PT - Journal Article PL - England TA - Nucleic Acids Res JT - Nucleic acids research JID - 0411011 RN - 0 (RNA-Binding Protein EWS) RN - 0 (EWSR1 protein, human) RN - 0 (RNA-Binding Proteins) RN - 0 (RNA, Messenger) SB - IM MH - *Protein Biosynthesis MH - Humans MH - *Endoplasmic Reticulum/metabolism/genetics MH - RNA-Binding Protein EWS MH - Homeostasis/genetics MH - *RNA-Binding Proteins/metabolism/genetics/chemistry MH - RNA, Messenger/metabolism/genetics MH - Unfolded Protein Response/genetics MH - Lipid Metabolism/genetics MH - Cell Line, Tumor EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:28 PHST- 2025/07/23 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:28 [entrez] AID - 9004256 [pii] AID - 10.1093/nar/gkag1022 [doi] PST - ppublish SO - Nucleic Acids Res. 2026 Oct 24;54(20):gkag1022. doi: 10.1093/nar/gkag1022. PMID- 42953961 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1362-4962 (Electronic) IS - 0305-1048 (Linking) VI - 54 IP - 20 DP - 2026 Oct 24 TI - DNA defense systems become a fitness liability during plasmid conjugation. LID - gkag1043 [pii] LID - 10.1093/nar/gkag1043 [doi] AB - Horizontal gene transfer via conjugative plasmids is a major driver of bacterial evolution. While antibiotic exposure selects for the resistance genes carried by some plasmids, much uncertainty remains about how plasmids persist and spread in microbial communities in the absence of such external selection. Here, we show that conjugative plasmid transfer selectively reduces the fitness of non-permissive recipients through the process of lethal zygosis: a phenomenon in which repeated Type IV Secretion System (T4SS) engagement damages recipients that fail to establish the plasmid. We found that this contact-dependent antagonism effectively clears a niche for plasmid donors by turning recipients' DNA defenses into a liability. Blocking establishment of the plasmid RK2 with CRISPR-Cas or restriction-modification caused strong fitness defects in Escherichia coli recipients by preventing expression of plasmid-encoded exclusion genes and leaving cells exposed to repeated T4SS engagement. We leveraged this insight to resolve an obstacle in bacterial editing by developing transposon vectors that co-deliver exclusion genes, increasing the efficiency of gene editing by orders of magnitude. Our results reveal the role of "coercive assimilation" in horizontal gene transfer, exposing a tradeoff where the advantage of bacterial immunity to foreign DNA is counterbalanced by increased susceptibility to lethal zygosis. CI - (c) The Author(s) 2026. Published by Oxford University Press. FAU - Martinson, Jonathan N V AU - Martinson JNV AD - Innovative Genomics Institute, University of California, Berkeley, CA 94720, United States. FAU - Song, Leo C T AU - Song LCT AD - Innovative Genomics Institute, University of California, Berkeley, CA 94720, United States. AD - Department of Comparative Biochemistry, University of California, Berkeley, CA 94720, United States. FAU - Rubin, Benjamin E AU - Rubin BE AUID- ORCID: 0000-0001-8684-2417 AD - Innovative Genomics Institute, University of California, Berkeley, CA 94720, United States. LA - eng GR - Lyda Hill Philanthropies/ GR - Acton Family Giving/ GR - Valhalla Foundation/ GR - Hastings/Quillin Fund/ GR - Silicon Valley Community Foundation/ GR - CH Foundation/ GR - Laura and Gary Lauder and Family/ GR - Sea Grape Foundation/ GR - Emerson Collective/ GR - Mike Schroepfer and Erin Hoffman Family Fund/ GR - G-2302-06692/Anne Wojcicki Foundation/ GR - Leona M. and Harry B. Helmsley Charitable Trust/ GR - m-CAFEs (Microbial Community Analysis & Functional Evaluation in Soils)/ GR - Lawrence Berkeley National Laboratory/ GR - DE-AC02-05CH11231/U.S. Department of Energy/ GR - Shurl and Kay Curci Foundation/ GR - Innovative Genomics Institute/ PT - Journal Article PL - England TA - Nucleic Acids Res JT - Nucleic acids research JID - 0411011 RN - 0 (Type IV Secretion Systems) RN - 0 (DNA Transposable Elements) RN - 0 (Extrachromosomal DNA) SB - IM MH - *Escherichia coli/genetics MH - *Plasmids/genetics MH - *Conjugation, Genetic MH - *Gene Transfer, Horizontal MH - *Genetic Fitness MH - Type IV Secretion Systems/genetics/metabolism MH - DNA Transposable Elements MH - CRISPR-Cas Systems MH - Extrachromosomal DNA EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:28 PHST- 2026/04/29 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:28 [entrez] AID - 9004251 [pii] AID - 10.1093/nar/gkag1043 [doi] PST - ppublish SO - Nucleic Acids Res. 2026 Oct 24;54(20):gkag1043. doi: 10.1093/nar/gkag1043. PMID- 42953959 OWN - NLM STAT- Publisher LR - 20261029 IS - 1613-6829 (Electronic) IS - 1613-6810 (Linking) DP - 2026 Oct 29 TI - Kernel Geometry Transformation by Controlled S Incorporation in Cu(14) Nanocluster and Its Impact on Electrocatalysis. LID - e76557 [pii] LID - 10.1002/smll.76557 [doi] AB - Cu nanoclusters have recently enabled the understanding of active sites and structural factors determining the electrocatalysis performance due to their atomic precision. However, the influence of structure geometry has so far remained elusive to Cu-nanoclusters-catalyzed electroreduction of CO(2). Here, we reported the controlled S incorporation based on the reductant dosage, achieving the geometry transformation of the Cu(8) kernel in the Cu(14) nanocluster while retaining the composition, outer motifs, and Cu valence state. Cu(14) with a parallelepiped kernel exhibits a distinct selectivity toward CH(4) (FE approximately 51%), which contrasts with Cu(14)-S containing a cubic kernel (mainly C(2+) products, FE approximately 33.8%). The electronic structures of Cu(14) nanoclusters are further revealed to be modulated by kernel geometry, altering the binding modes or energetics of the intermediates, as evidenced by in situ spectroscopy and theoretical calculation. CI - (c) 2026 Wiley-VCH GmbH. FAU - Zhu, Baohuan AU - Zhu B AUID- ORCID: 0009-0003-3160-6646 AD - Institute of Energy, Hefei Comprehensive National Science Center (Anhui Energy Laboratory), Hefei, People's Republic of China. AD - Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Molecular Sciences, Anhui Normal University, Wuhu, People's Republic of China. FAU - Zhang, Yingjie AU - Zhang Y AD - School of Chemistry and Chemical Engineering, Shandong University of Technology, Zibo, Shandong, People's Republic of China. FAU - Li, Yang AU - Li Y AD - Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Molecular Sciences, Anhui Normal University, Wuhu, People's Republic of China. FAU - Zhu, Zihao AU - Zhu Z AD - Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Molecular Sciences, Anhui Normal University, Wuhu, People's Republic of China. FAU - Guan, Yajie AU - Guan Y AD - Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Molecular Sciences, Anhui Normal University, Wuhu, People's Republic of China. FAU - Wang, Likai AU - Wang L AUID- ORCID: 0000-0002-9898-3839 AD - School of Chemistry and Chemical Engineering, Shandong University of Technology, Zibo, Shandong, People's Republic of China. FAU - Bi, Wentuan AU - Bi W AUID- ORCID: 0009-0003-1655-8412 AD - Institute of Energy, Hefei Comprehensive National Science Center (Anhui Energy Laboratory), Hefei, People's Republic of China. FAU - Xia, Nan AU - Xia N AUID- ORCID: 0000-0003-2877-5070 AD - Key Laboratory of Functional Molecular Solids, Ministry of Education, College of Chemistry and Molecular Sciences, Anhui Normal University, Wuhu, People's Republic of China. LA - eng GR - 22171268 22175051 21805170/National Natural Science Foundation of China/ GR - 2408085Y011/Anhui Provincial Natural Science Foundation/ GR - 2022HSC-CIP018/Collaborative Innovation Program of Hefei Science Center, CAS/ GR - 25KZS208/Institute of Energy, Hefei Comprehensive National Science Center (Anhui Energy Laboratory)/ PT - Journal Article DEP - 20261029 PL - Germany TA - Small JT - Small (Weinheim an der Bergstrasse, Germany) JID - 101235338 SB - IM OTO - NOTNLM OT - Cu nanoclusters OT - S incorporation OT - binding energetics OT - electrocatalysis OT - kernel geometry EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:28 PHST- 2026/10/08 00:00 [revised] PHST- 2026/08/14 00:00 [received] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:28 [entrez] AID - e76557 [pii] AID - 10.1002/smll.76557 [doi] PST - aheadofprint SO - Small. 2026 Oct 29:e76557. doi: 10.1002/smll.76557. PMID- 42953954 OWN - NLM STAT- Publisher LR - 20261029 IS - 1471-0528 (Electronic) IS - 1470-0328 (Linking) DP - 2026 Oct 29 TI - From Delays to Systems in Maternal Healthcare Utilisation: A Conceptual Framework Study. LID - 10.1111/1471-0528.70368 [doi] AB - OBJECTIVE: To develop an operational, systems-oriented framework for analysing sustainable maternal healthcare utilisation, grounded in Nigerian evidence and intended for contextual adaptation and testing. DESIGN: Conceptual framework study using narrative synthesis, structured comparative evidence synthesis and independent face and content appraisal. SETTING: Nigeria, with consideration of applicability to other maternal healthcare settings. POPULATION OR SAMPLE: Purposively selected peer-reviewed Nigerian maternal healthcare studies; three independent external appraisers comprising two maternal-health researchers and one healthcare professional. METHODS: We synthesised empirical evidence into linked domains and hypothesised relationships, compared it with established maternal-health and health-systems frameworks and challenged it against additional Nigerian studies. Appraisers assessed relevance, clarity, completeness, cross-domain plausibility, and practical utility. MAIN OUTCOME MEASURES: Conceptual coherence, domain relevance, completeness, explanatory plausibility, practical utility, and candidate indicators of sustainable maternal healthcare utilisation; no patient-level clinical outcomes were measured. RESULTS: We specified six linked domains: policy and governance, health-system capacity, community engagement, equity, quality of care, and monitoring, evaluation and learning. Governance provides structural conditions; capacity, quality and community engagement interact; equity modifies pathways; and monitoring supports feedback and adaptation. External appraisal offered qualified support and identified boundaries requiring refinement. CONCLUSIONS: The framework offers a testable systems structure for diagnosing interacting barriers and guiding context-appropriate indicators and multilevel interventions. Prospective testing is needed to establish construct validity, responsiveness and transferability. CI - (c) 2026 The Author(s). BJOG: An International Journal of Obstetrics and Gynaecology published by John Wiley & Sons Ltd. FAU - Ekpenyong, Mandu Stephen AU - Ekpenyong MS AUID- ORCID: 0000-0003-3778-645X AD - Manchester Metropolitan University, Manchester, UK. FAU - Matheson, David AU - Matheson D AD - University of Wolverhampton, School of Sport, Walsall, UK. FAU - Serrant, Laura AU - Serrant L AD - Anglia Ruskin University, Chelmsford, UK. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - BJOG JT - BJOG : an international journal of obstetrics and gynaecology JID - 100935741 SB - IM OTO - NOTNLM OT - Nigeria OT - conceptual framework OT - equity OT - health systems OT - maternal health OT - maternal healthcare utilisation OT - quality of care EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:28 PHST- 2026/10/01 00:00 [revised] PHST- 2026/05/28 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:28 [entrez] AID - 10.1111/1471-0528.70368 [doi] PST - aheadofprint SO - BJOG. 2026 Oct 29. doi: 10.1111/1471-0528.70368. PMID- 42953953 OWN - NLM STAT- Publisher LR - 20261029 IS - 1095-8290 (Electronic) IS - 0305-7364 (Linking) DP - 2026 Oct 29 TI - Evidence of parallel multi-dimensional phenotypic reorganization in Brassica incana on two Mediterranean islands. LID - mcag347 [pii] LID - 10.1093/aob/mcag347 [doi] AB - BACKGROUND AND AIMS: Island populations represent natural evolutionary experiments, often showing predictable divergence in life-history strategies (i.e., island syndrome). The multi-dimensional expression of this island syndrome in plants, spanning vegetative morphology, floral architecture, and seed provisioning, and its downstream consequences for offspring performance remains poorly characterized. Here, we aim to investigate whether island colonization reshapes phenotypic traits and reproductive investment strategies in insular Brassica incana populations occurring on two proximate Mediterranean islands. METHODS: Focusing on two mainland-insular population pairs across contrasting substrates (volcanic and limestone), we integrated genomic, phenotypic, and experimental approaches. We measured vegetative, floral, and reproductive traits across two flowering seasons, estimated standardized linear selection gradients to identify which phenotypic traits are under selection, and estimated the strength of phenotypic integration, i.e., the correlation among phenotypic traits, within each population. Finally, we tested the effect of seed mass on germination probability and early seedling performance in a controlled experiment. KEY RESULTS: Insular populations showed moderate genomic differentiation from mainland populations and comparable levels of genetic diversity. Across both mainland-insular comparisons and both flowering seasons, insular populations showed larger leaf areas, larger corollas, a higher ratio of reproductive to aboveground vegetative biomass proxy, more seeds per fruit, and heavier seeds than mainland populations, while producing fewer leaves and fewer fruits, revealing different allocation trade-offs. Corolla size was under significant positive selection in insular populations. Seed mass did not predict germination probability, but was a strong positive predictor of seedling biomass under home-soil conditions. Phenotypic integration was overall low across all populations. CONCLUSIONS: Island colonization in B. incana shows a consistent multi-dimensional syndrome across two independent colonization events and across years. This replicated demonstration expands our understanding of the island syndrome in plants, while revealing small and proximate Mediterranean islands as valuable, underexplored contexts for studying insular evolution. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Scopece, Giovanni AU - Scopece G AUID- ORCID: 0000-0003-0341-6231 AD - Department of Biology, University of Naples Federico II, Naples, Italy. FAU - Laccetti, Lucrezia AU - Laccetti L AUID- ORCID: 0000-0001-8578-6644 AD - Department of Biology, University of Naples Federico II, Naples, Italy. AD - Area of Biodiversity and Conservation, Department of Biology, Universidad Rey Juan Carlos, ESCET, Mostoles, Madrid, Spain. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Ann Bot JT - Annals of botany JID - 0372347 SB - IM OTO - NOTNLM OT - Aboveground vegetative biomass OT - Insular evolution OT - Island syndrome OT - Reproductive allocation OT - Seed mass EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:28 PHST- 2026/06/24 00:00 [received] PHST- 2026/10/20 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:28 [entrez] AID - 9004290 [pii] AID - 10.1093/aob/mcag347 [doi] PST - aheadofprint SO - Ann Bot. 2026 Oct 29:mcag347. doi: 10.1093/aob/mcag347. PMID- 42953939 OWN - NLM STAT- Publisher LR - 20261029 IS - 1521-4095 (Electronic) IS - 0935-9648 (Linking) DP - 2026 Oct 29 TI - Life-Cycle-Sustainable Capacitive Deionization Enabled by Organic Molecular Engineering for Selective Ammonium Capture. LID - e75566 [pii] LID - 10.1002/adma.75566 [doi] AB - Selective ammonium (NH(4) (+)) recovery from complex wastewater streams remains a pivotal challenge in sustainable nitrogen management. Capacitive deionization (CDI) offers an energy-efficient electrochemical route for ion separation, yet its practical application is constrained by the inherent trade-off between NH(4) (+) adsorption capacity and selectivity. Herein, we circumvent this fundamental limitation through the rational molecular engineering of an organic electroactive material, 1,4-bis(1H-imidazo[4,5-b]phenazin-2-yl)benzene (IPB), which features extended electron delocalization and abundant C horizontal lineN moieties for NH(4) (+)-specific redox-coupled capture. The resulting IPB-based CDI prototype delivers a high NH(4) (+) adsorption capacity of 267.23 mg g(-1) and a fast rate of 62.51 mg g(-1) min(-1), while sustaining remarkable selectivity in complex multi-ion matrices, thereby effectively surpassing the conventional capacity-selectivity trade-off. Notably, CDI adsorption reduces wastewater NH(4) (+) levels to below 3 mg L(-1) and achieves 82.3% direct recovery as liquid fertilizer during CDI desorption, as demonstrated by isotope tracing experiments. The CDI process exhibits a low carbon footprint of only 4.66 x 10(-2) t CO(2)-equivalent per tonne of NH(4) (+) treated, underscoring its superior life-cycle sustainability. Therefore, this work proposes a molecularly engineered organic electrode material for high-performance wastewater remediation and circular nutrient recovery, paving the way toward carbon-neutral electrochemical water treatment technologies. CI - (c) 2026 Wiley-VCH GmbH. FAU - Yuan, Tingliang AU - Yuan T AD - School of Materials Science and Engineering, Jiangsu University of Science and Technology, Zhenjiang, P. R. China. FAU - Yang, Jun AU - Yang J AUID- ORCID: 0000-0003-4463-5633 AD - School of Materials Science and Engineering, Jiangsu University of Science and Technology, Zhenjiang, P. R. China. FAU - Li, Chunyan AU - Li C AD - School of Materials Science and Engineering, Jiangsu University of Science and Technology, Zhenjiang, P. R. China. FAU - Peng, Jinyang AU - Peng J AD - School of Materials Science and Engineering, Sun Yat-Sen University, Guangzhou, P. R. China. FAU - Shi, Minjie AU - Shi M AUID- ORCID: 0000-0002-6568-3215 AD - School of Materials Science and Engineering, Jiangsu University of Science and Technology, Zhenjiang, P. R. China. FAU - Yan, Xingbin AU - Yan X AUID- ORCID: 0000-0002-9976-8815 AD - School of Materials Science and Engineering, Sun Yat-Sen University, Guangzhou, P. R. China. LA - eng GR - BK20251919/Basic Research Program of Jiangsu Province/ GR - 22579193/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261029 PL - Germany TA - Adv Mater JT - Advanced materials (Deerfield Beach, Fla.) JID - 9885358 SB - IM OTO - NOTNLM OT - ammonium recovery OT - electrochemical separation OT - electrode materials OT - organic molecule OT - selectivity EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:26 PHST- 2026/10/01 00:00 [revised] PHST- 2026/07/13 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:26 [entrez] AID - e75566 [pii] AID - 10.1002/adma.75566 [doi] PST - aheadofprint SO - Adv Mater. 2026 Oct 29:e75566. doi: 10.1002/adma.75566. PMID- 42953919 OWN - NLM STAT- Publisher LR - 20261029 IS - 1365-3016 (Electronic) IS - 0269-5022 (Linking) DP - 2026 Oct 29 TI - Reported Factors Associated With Hearing Loss in Infants Admitted to Neonatal Intensive Care Units: A Scoping Review of the Evidence and Its Limitations. LID - 10.1111/ppe.70212 [doi] AB - BACKGROUND: Hearing loss (HL) is far more common in neonatal intensive care unit (NICU) graduates than in well-baby populations. Although many broadly defined 'risk factors' have been reported, their causal relevance remains unclear. OBJECTIVES: We conducted a scoping review to synthesise reported factors for HL among NICU and special care nursery (SCN) infants. We then assessed the strength of the supporting evidence. DATA SOURCES: Embase, Medline, APA PsycInfo, and Web of Science were searched (1 Jan 2015-11 Mar 2025). STUDY SELECTION AND DATA EXTRACTION: We included studies that reported factors associated with HL among NICU/SCN graduates. Commentaries, conference abstracts, letters, and case reports/series were excluded. Data were extracted independently by two reviewers using a piloted Excel spreadsheet. SYNTHESIS: We followed PRISMA-ScR guidelines. Reported factors were classified into categories and broad domains. We summarised adjusted associations and appraised the evidence, including indications of causal support. RESULTS: Ninety-six studies met the inclusion criteria, identifying 141 unique factors which were organised into six domains and 32 categories. No eligible study focused specifically on SCN infants. The most frequently studied factors were birthweight (38.5%), hyperbilirubinemia (25.0%), Apgar score (24.0%), sepsis (21.9%), and sex (20.8%). Effect estimates were infrequently reported, and evidence within categories was highly heterogeneous. Support for causal interpretation was identified in four studies (4.2%), including those of hypoxia-ischemia, encephalopathy with seizures, postnatal CMV infection, and higher frequency of vancomycin dosing. Evidence was limited by the small number of confounder-adjusted analyses, prospective or trial designs, and studies with adequate follow-up. CONCLUSIONS: The literature on NICU-related hearing loss includes many repeatedly reported associated factors, but the evidence supporting their causal role remains limited and heterogeneous. As a result, many reported factors cannot yet be interpreted as credible prevention targets. Better-designed studies for estimating causal effects are needed to support prevention-oriented decision-making. CI - (c) 2026 The Author(s). Paediatric and Perinatal Epidemiology published by John Wiley & Sons Ltd. FAU - Bai, Jinzi AU - Bai J AUID- ORCID: 0009-0007-3559-4776 AD - Murdoch Children's Research Institute, the Royal Children's Hospital, Parkville, Victoria, Australia. AD - Department of Paediatrics, The University of Melbourne, Parkville, Victoria, Australia. FAU - Chandrasekaran, Preethi AU - Chandrasekaran P AD - Murdoch Children's Research Institute, the Royal Children's Hospital, Parkville, Victoria, Australia. AD - Department of Paediatrics, The University of Melbourne, Parkville, Victoria, Australia. AD - Monash Newborn, Monash Children's Hospital, Clayton, Victoria, Australia. FAU - Shepherd, Daisy AU - Shepherd D AUID- ORCID: 0000-0001-8540-0473 AD - Murdoch Children's Research Institute, the Royal Children's Hospital, Parkville, Victoria, Australia. AD - Department of Paediatrics, The University of Melbourne, Parkville, Victoria, Australia. FAU - Wake, Melissa AU - Wake M AUID- ORCID: 0000-0001-7501-9257 AD - Murdoch Children's Research Institute, the Royal Children's Hospital, Parkville, Victoria, Australia. AD - Department of Paediatrics, The University of Melbourne, Parkville, Victoria, Australia. FAU - Sung, Valerie AU - Sung V AUID- ORCID: 0000-0003-2029-6331 AD - Murdoch Children's Research Institute, the Royal Children's Hospital, Parkville, Victoria, Australia. AD - Department of Paediatrics, The University of Melbourne, Parkville, Victoria, Australia. AD - The Royal Children's Hospital, Parkville, Australia. FAU - Wang, Jing AU - Wang J AUID- ORCID: 0000-0001-5701-476X AD - Murdoch Children's Research Institute, the Royal Children's Hospital, Parkville, Victoria, Australia. LA - eng GR - Australian Government Research Training Program (RTP) Scholarship/ GR - Lorenzo and Pamela Galli Charitable Trust/ GR - NCRI000077/Medical Research Future Fund/ GR - MRFRDIII000002/Medical Research Future Fund/ GR - GNT1160906/National Health and Medical Research Council/ GR - GNT2033683/National Health and Medical Research Council/ GR - Melbourne Children's Clinician Scientist Fellowship/ GR - Jack Brockhoff Foundation/ GR - Murdoch Children's Research Institute Generation Victoria Postdoctoral Fellowship/ PT - Journal Article PT - Review DEP - 20261029 PL - England TA - Paediatr Perinat Epidemiol JT - Paediatric and perinatal epidemiology JID - 8709766 SB - IM OTO - NOTNLM OT - causal inference OT - hearing loss OT - neonatal intensive care unit OT - risk factors OT - scoping review EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 CRDT- 2026/10/29 06:25 PHST- 2026/10/12 00:00 [revised] PHST- 2026/06/17 00:00 [received] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:25 [entrez] AID - 10.1111/ppe.70212 [doi] PST - aheadofprint SO - Paediatr Perinat Epidemiol. 2026 Oct 29. doi: 10.1111/ppe.70212. PMID- 42953910 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1435-8115 (Electronic) IS - 1431-9276 (Linking) VI - 32 IP - 6 DP - 2026 Oct 12 TI - Preservation of Vascular Tissues From Shrinkage During X-Ray 3D-CECT Imaging. LID - ozag120 [pii] LID - 10.1093/mam/ozag120 [doi] AB - Preventing shrinkage of biological tissues during X-ray microfocus computed tomography (3D-microCT) is essential to prevent motion artifacts and microstructural changes, which is particularly problematic in the context of 4D-microCT. While liquid immersion and parafilm wrapping are commonly used to limit dehydration, both methods present limitations for combining them with in situ mechanical testing. This work introduces an in-house environmental stage capable of controlling temperature and relative humidity (RH) around the sample during imaging with vapor phase method. Using 3D-microCT with stained porcine aortic tissues, we assessed the impact of temperature and RH on tissue volume change over time. Results demonstrated that both temperature and RH significantly influence shrinkage rate, with temperature playing a dominant role. Volume loss increased with decreasing RH and increasing temperature. Furthermore, shrinkage was heterogeneous and was particularly pronounced at cut tissue edges. Among all tested conditions, 10 degrees C combined with 95% RH best preserved tissue hydration, showing minimal shrinkage and reduced motion artifacts. Validation at higher spatial resolution confirmed that this controlled environment enables prolonged acquisitions while maintaining image quality superior to imaging under ambient conditions. This study highlights the importance of environmental control during microCT imaging of soft tissues and provides a quantitative framework for selecting optimal atmospheric conditions. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Microscopy Society of America. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Mazy, Lara AU - Mazy L AD - MEED Department, Institute of Mechanics, Materials and Civil Engineering, UCLouvain, Place du Levant 2 - box L5.04.01, 1348 Louvain-la-Neuve, Belgium. AD - Department of Materials Engineering, KU Leuven, Kasteelpark Arenberg 44 - box 2450, 3001 Leuven, Belgium. AD - Pole of Morphology, Institute of Experimental and Clinical Research, UCLouvain, Avenue E. Mounier 52 - box B1.52.04, 1200 Brussels, Belgium. FAU - Pyka, Grzegorz AU - Pyka G AD - MEED Department, Institute of Mechanics, Materials and Civil Engineering, UCLouvain, Place du Levant 2 - box L5.04.01, 1348 Louvain-la-Neuve, Belgium. FAU - Soete, Jeroen AU - Soete J AUID- ORCID: 0000-0003-3357-4070 AD - Department of Materials Engineering, KU Leuven, Kasteelpark Arenberg 44 - box 2450, 3001 Leuven, Belgium. FAU - Wevers, Martine AU - Wevers M AD - Department of Materials Engineering, KU Leuven, Kasteelpark Arenberg 44 - box 2450, 3001 Leuven, Belgium. FAU - Kerckhofs, Greet AU - Kerckhofs G AUID- ORCID: 0000-0002-1750-8324 AD - MEED Department, Institute of Mechanics, Materials and Civil Engineering, UCLouvain, Place du Levant 2 - box L5.04.01, 1348 Louvain-la-Neuve, Belgium. AD - Department of Materials Engineering, KU Leuven, Kasteelpark Arenberg 44 - box 2450, 3001 Leuven, Belgium. AD - Pole of Morphology, Institute of Experimental and Clinical Research, UCLouvain, Avenue E. Mounier 52 - box B1.52.04, 1200 Brussels, Belgium. AD - Prometheus, Division of Skeletal Tissue Engineering, KU Leuven, O&N I Herestraat 49 - box 813, 3000 Leuven, Belgium. LA - eng GR - G088020/FWO/ GR - SB-FWO 1S61625N/FWO/ GR - S007219N/FWO/ GR - FWF/ GR - Research Foundation Flanders/ GR - FSR/ GR - Fonds de Recherche Special Jeunes Acad emiques/ GR - Federation Wallonie-Bruxelles/ GR - Action de Recherche Concertee/ GR - 19/24-097/ARC/ GR - 18/23-094/ARC/ GR - FNRS/ GR - Credit de Recherche/ GR - 40028871/CDR/ GR - microCT facility of Uclouvain/ PT - Journal Article PL - England TA - Microsc Microanal JT - Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada JID - 9712707 SB - IM MH - Animals MH - Swine MH - Humidity MH - Temperature MH - *X-Ray Microtomography/methods MH - *Aorta/diagnostic imaging MH - *Imaging, Three-Dimensional/methods OTO - NOTNLM OT - biological tissues OT - contrast enhancement OT - dehydration OT - environmental stage OT - mechanical properties OT - microCT COIS- Conflict of Interest: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:24 PHST- 2026/03/20 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:24 [entrez] AID - 9004243 [pii] AID - 10.1093/mam/ozag120 [doi] PST - ppublish SO - Microsc Microanal. 2026 Oct 12;32(6):ozag120. doi: 10.1093/mam/ozag120. PMID- 42953909 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1473-0766 (Electronic) IS - 0951-3590 (Linking) VI - 42 IP - 1 DP - 2026 Dec 31 TI - Polycystic ovary syndrome research in sub-Saharan Africa: a systematic review on current status and future directions. PG - 2750762 LID - 10.1080/09513590.2026.2750762 [doi] AB - OBJECTIVES: Polycystic ovary syndrome (PCOS) is one of the most prevalent disorders affecting women. The status of PCOS research in sub-Saharan Africa is unknown, where non-communicable diseases are prevalent but often underrecognized. To assess current knowledge about PCOS in sub-Saharan Africa and identify gaps in the literature. METHODS: A systematic search was conducted across PubMed, Embase, Cochrane, and African Journals Online through 9 August 2026 to identify original studies on PCOS in sub-Saharan Africa. Original research studies involving sub-Saharan African populations, without age-related constraints, were eligible. We excluded non-original studies and studies that did not focus on PCOS. Three independent reviewers (S.H., M.A., and C.C.M.) performed the searches. RESULTS: Of 1973 retrieved records, 40 studies met the inclusion criteria, predominantly from Nigeria, with additional contributions from South Africa, Cameroon, the Democratic Republic of Congo, Tanzania, Benin, Kenya, and Ethiopia. Most studies were observational and primarily focused on PCOS in the context of infertility. No population-based prevalence studies were identified; however, 13 studies reported PCOS prevalence, mostly in clinically selected populations. CONCLUSIONS: PCOS remains understudied in sub-Saharan Africa, with substantial gaps in population-based epidemiology, diagnostic practices, phenotype distribution, management, and metabolic outcomes. Addressing these gaps through larger, well-designed studies is critical to accurately assess the disease burden, inform clinical guidelines, and optimize management strategies. Additionally, overcoming barriers such as cultural attitudes, cost, and limited accessibility to diagnostic tools and treatment options remains essential to improving PCOS care and research across the region.PROSPERO registration ID CRD420250649730 - 13 March 2025. FAU - Hatoum, Sana AU - Hatoum S AUID- ORCID: 0000-0001-7084-7846 AD - Foundation for Research and Education Excellence, Vestavia, AL, USA. FAU - Amiri, Mina AU - Amiri M AUID- ORCID: 0000-0002-1126-9297 AD - Foundation for Research and Education Excellence, Vestavia, AL, USA. AD - School of Nursing & Midwifery, Tehran University of Medical Sciences (TUMS), Tehran, Iran. AD - Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, TUMS, Tehran, Iran. FAU - Makwe, Christian Chigozie AU - Makwe CC AD - Department of Obstetrics and Gynecology, College of Medicine, University of Lagos, Lagos, Nigeria. FAU - Olamijulo, Joseph Ayodeji AU - Olamijulo JA AD - Department of Obstetrics and Gynecology, College of Medicine, University of Lagos, Lagos, Nigeria. FAU - Azziz, Ricardo AU - Azziz R AUID- ORCID: 0000-0002-3917-0483 AD - Foundation for Research and Education Excellence, Vestavia, AL, USA. AD - Department of Ob/Gyn, Heersink School of Medicine, University of Alabama at Birmingham (UAB), Birmingham, AL, USA. AD - Department of Medicine, Heersink School of Medicine, UAB, Birmingham, AL, USA. AD - Department of Healthcare Organization & Policy, School of Public Health, UAB, Birmingham, AL, USA. AD - Department of Health Policy, Management, and Behavior, School of Public Health, University at Albany, SUNY, Rensselaer, NY, USA. LA - eng PT - Journal Article PT - Review PT - Systematic Review DEP - 20261029 PL - England TA - Gynecol Endocrinol JT - Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology JID - 8807913 SB - IM MH - Humans MH - *Polycystic Ovary Syndrome/epidemiology MH - Female MH - Africa South of the Sahara/epidemiology MH - Prevalence MH - Sub-Saharan African People MH - *Biomedical Research/trends OTO - NOTNLM OT - Polycystic ovary syndrome OT - health disparities OT - research gaps OT - sub-Saharan Africa OT - systematic review EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 CRDT- 2026/10/29 06:24 PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:24 [entrez] AID - 10.1080/09513590.2026.2750762 [doi] PST - ppublish SO - Gynecol Endocrinol. 2026 Dec 31;42(1):2750762. doi: 10.1080/09513590.2026.2750762. Epub 2026 Oct 29. PMID- 42953893 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2326-3253 (Print) IS - 2326-3253 (Electronic) IS - 2326-3253 (Linking) VI - 13 IP - 11 DP - 2026 Nov TI - Successful Endoscopic Reduction of Gastric Volvulus Associated With Diaphragmatic Hernia Using a Short-type Enteroscope. PG - e02381 LID - 10.14309/crj.0000000000002381 [doi] LID - e02381 AB - Gastric volvulus is a rare but life-threatening medical emergency. Emergency endoscopic reduction serves as a crucial less-invasive option. However, conventional upper endoscopes often fail to achieve detorsion due to insufficient length and rigidity. We report a case of gastric volvulus secondary to a large diaphragmatic hernia. Emergency endoscopic reduction was successfully achieved using a short-type enteroscope, which provided potential advantages in passive bending, torque transmission, and adequate length to safely pass the pylorus and reach the third portion of the duodenum. Following endoscopic detorsion and decompression, the patient underwent laparoscopic diaphragmatic hernia repair and anterior gastropexy with an uneventful recovery. CI - (c) 2026 The Author(s). Published by Wolters Kluwer Health, LLC. on behalf of The American College of Gastroenterology. FAU - Sato, Kazuki AU - Sato K AD - Division of Gastroenterology, Shin-kuki General Hospital, Saitama, Japan. FAU - Ishii, Naoki AU - Ishii N AUID- ORCID: 0000-0003-4876-1063 AD - Division of Gastroenterology, Shin-kuki General Hospital, Saitama, Japan. FAU - Ouchida, Yuzuki AU - Ouchida Y AD - Division of Surgery, Shin-kuki General Hospital, Saitama, Japan. FAU - Kato, Takuya AU - Kato T AD - Division of Surgery, Shin-kuki General Hospital, Saitama, Japan. FAU - Noda, Kazumasa AU - Noda K AD - Division of Surgery, Shin-kuki General Hospital, Saitama, Japan. LA - eng PT - Case Reports PT - Journal Article DEP - 20261028 PL - United States TA - ACG Case Rep J JT - ACG case reports journal JID - 101638398 PMC - PMC13716423 OTO - NOTNLM OT - diaphragmatic hernia OT - enteroscope OT - gastric volvulus EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/28 CRDT- 2026/10/29 06:22 PHST- 2026/07/13 00:00 [received] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:22 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - ACGCR-26-0956 [pii] AID - 10.14309/crj.0000000000002381 [doi] PST - epublish SO - ACG Case Rep J. 2026 Oct 28;13(11):e02381. doi: 10.14309/crj.0000000000002381. eCollection 2026 Nov. PMID- 42953887 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2035-8164 (Electronic) IS - 2035-8237 (Print) IS - 2035-8164 (Linking) VI - 18 DP - 2026 TI - HEMIARTHROPLASTY FOR PROXIMAL FEMORAL FRACTURES IN CENTENARIANS: A CASE SERIES WITH 12-MONTH SURVIVAL AND FUNCTIONAL OUTCOMES. PG - 172709 LID - 10.52965/001c.172709 [doi] LID - 172709 AB - BACKGROUND: Hip fractures in centenarians are associated with high mortality, poor functional recovery, and substantial perioperative risk. Data specifically describing survival and functional outcomes after hemiarthroplasty in this exceptionally old population remain limited. SUBJECTS AND METHODS: We conducted a retrospective descriptive case series of 16 patients aged 100 years and older with proximal femoral fractures who underwent hemiarthroplasty at Viet Duc University Hospital between January 2021 and January 2025. Patients were managed within a multidisciplinary care pathway incorporating orthogeriatric principles. Data collected included comorbidity burden, pre-injury mobility and functional status, perioperative characteristics, postoperative complications, survival, and functional outcomes. Survival status, mobility, and functional independence were assessed at 1, 3, and 12 months after surgery. Functional outcomes were assessed among surviving patients at each follow-up time point. RESULTS: The mean age was 101.75 +/- 1.88 years, and 93.75% of patients were female. Before injury, the mean Parker Mobility Score was 4.06 +/- 0.92 and the mean Barthel Index was 81.56 +/- 7.47. The mean time from admission to surgery was 45.38 +/- 15.28 hours. Delirium was the most common postoperative medical complication (37.50%), followed by pneumonia (12.50%), and no in-hospital deaths were recorded. Cumulative mortality was 6.25% at 1 month, 12.50% at 3 months, and 31.25% at 12 months. Among survivors, the mean Parker Mobility Score decreased to 1.47 +/- 1.13 at 1 month and subsequently improved to 2.50 +/- 1.87 at 3 months and 3.09 +/- 2.26 at 12 months. The mean Barthel Index showed a similar pattern, decreasing to 36.00 +/- 17.94 at 1 month and improving to 48.21 +/- 24.54 at 3 months and 52.73 +/- 25.43 at 12 months. Both measures remained below pre-injury levels at 12 months. CONCLUSIONS: In this descriptive case series of centenarian patients selected for hemiarthroplasty, cumulative 12-month mortality was 31.25%. Among survivors, mobility and functional independence improved progressively after the first postoperative month but remained below pre-injury levels at 12 months. These findings highlight the importance of considering functional recovery alongside survival when evaluating outcomes in this exceptionally old patient population. FAU - Nguyen, Manh Khanh AU - Nguyen MK AD - Institute of Orthopaedics and Trauma Surgery Viet Duc Hospital. ROR: https://ror.org/049ymah45 AD - VNU University of Medicine and Pharmacy Vietnam National University, Hanoi. ROR: https://ror.org/02jmfj006 FAU - Dang, Van Long AU - Dang VL AD - Institute of Orthopaedics and Trauma Surgery Viet Duc Hospital. ROR: https://ror.org/049ymah45 FAU - Nguyen, Van Hoc AU - Nguyen VH AD - Institute of Orthopaedics and Trauma Surgery Viet Duc Hospital. ROR: https://ror.org/049ymah45 AD - VNU University of Medicine and Pharmacy Vietnam National University, Hanoi. ROR: https://ror.org/02jmfj006 FAU - Nguyen, Hoang Quan AU - Nguyen HQ AD - Institute of Orthopaedics and Trauma Surgery Viet Duc Hospital. ROR: https://ror.org/049ymah45 FAU - Do, Vu Anh AU - Do VA AD - Institute of Orthopaedics and Trauma Surgery Viet Duc Hospital. ROR: https://ror.org/049ymah45 FAU - Do, Van Hai AU - Do VH AD - Institute of Orthopaedics and Trauma Surgery Viet Duc Hospital. ROR: https://ror.org/049ymah45 FAU - Nguyen, Moc Son AU - Nguyen MS AD - Institute of Orthopaedics and Trauma Surgery Viet Duc Hospital. ROR: https://ror.org/049ymah45 AD - Hanoi Medical University. ROR: https://ror.org/01n2t3x97 FAU - Cao, Dinh Bang AU - Cao DB AD - Institute of Orthopaedics and Trauma Surgery Viet Duc Hospital. ROR: https://ror.org/049ymah45 FAU - Nguyen, Huy Thiep AU - Nguyen HT AD - Institute of Orthopaedics and Trauma Surgery Viet Duc Hospital. ROR: https://ror.org/049ymah45 FAU - Le, Xuan Hoang AU - Le XH AD - Institute of Orthopaedics and Trauma Surgery Viet Duc Hospital. ROR: https://ror.org/049ymah45 FAU - Nguyen, Manh Khoi AU - Nguyen MK AD - Hanoi Medical University. ROR: https://ror.org/01n2t3x97 LA - eng PT - Journal Article DEP - 20261026 PL - United States TA - Orthop Rev (Pavia) JT - Orthopedic reviews JID - 101524779 PMC - PMC13716521 OTO - NOTNLM OT - Hemiarthroplasty OT - centenarian OT - mobility OT - mobility recovery OT - multidisciplinary care OT - proximal femoral fracture EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/26 CRDT- 2026/10/29 06:21 PHST- 2026/07/19 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:21 [entrez] PHST- 2026/10/26 00:00 [pmc-release] AID - 172709 [pii] AID - 10.52965/001c.172709 [doi] PST - epublish SO - Orthop Rev (Pavia). 2026 Oct 26;18:172709. doi: 10.52965/001c.172709. eCollection 2026. PMID- 42953881 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2035-8164 (Electronic) IS - 2035-8237 (Print) IS - 2035-8164 (Linking) VI - 18 DP - 2026 TI - Endoscopic versus non-endoscopic decompression for degenerative lumbar spinal stenosis in adults: a systematic review and meta-analysis of randomised trials. PG - 172721 LID - 10.52965/001c.172721 [doi] LID - 172721 AB - INTRODUCTION: Endoscopic decompression may reduce morbidity, but sustained benefits over non-endoscopic surgery remain uncertain. OBJECTIVE: To assess disability, pain and harms in adult randomised comparisons of decompression without fusion. METHODS: MEDLINE, Embase, CINAHL and the Cochrane Library were searched on 23 September 2026. English-language adult randomised trials comparing endoscopic and non-endoscopic decompression without fusion were eligible. Two reviewers independently selected studies and extracted data. Bias and certainty were assessed with the revised Cochrane risk-of-bias tool and the Grading of Recommendations Assessment, Development and Evaluation approach. Compatible estimates used random-effects models with modified Hartung-Knapp intervals. Registration and scope changes were retrospective (Open Science Framework: https://osf.io/qb9a6/). The review received no funding. RESULTS: Sixteen reports described 12 trials allocating 1,212 participants. The protocol-planned 12-month Oswestry Disability Index analysis included four trials (302 participants): mean difference -4.54 points (95% confidence interval -5.95 to -3.13). Zhang F supplied 95.1% of the weight and had reporting concerns. The post hoc sensitivity without that trial included three microscopic comparisons (234 participants): -3.74 (-12.30 to 4.81; low certainty). Six-month disability and exploratory pain analyses were similarly sensitive or uncertain. Nine trials reported 23 durotomies (risk ratio 0.61, 95% confidence interval 0.23 to 1.59; very low certainty). Inconsistent measurement limited blood-loss and reoperation comparisons. CONCLUSION: Endoscopic decompression has not shown a reliable sustained advantage in disability, pain or safety. The small pooled disability benefit depended heavily on one trial with reporting concerns. Procedure selection should consider the required decompression and surgical expertise; these findings do not establish equivalent safety. FAU - Pammi, Srinath AU - Pammi S AUID- ORCID: 0000-0002-3618-1945 AD - Trauma and Orthopaedics Basildon University Hospital. FAU - Jayaram, Jithuram AU - Jayaram J AD - Trauma and Orthopaedics Bedford Hospital. ROR: https://ror.org/045s3rx57 FAU - Rajan, Praveen AU - Rajan P AD - Trauma and Orthopaedics Basildon University Hospital. FAU - Balineni, Bhargava Krishna AU - Balineni BK AD - Trauma and Orthopaedics Basildon University Hospital. FAU - Akula, Maheswara AU - Akula M AD - Trauma and Orthopaedics Basildon University Hospital. FAU - Landes, Kelsey AU - Landes K AD - Broomfield Healthcare Library. LA - eng PT - Journal Article DEP - 20261026 PL - United States TA - Orthop Rev (Pavia) JT - Orthopedic reviews JID - 101524779 PMC - PMC13716509 OTO - NOTNLM OT - Systematic review OT - disability OT - endoscopic decompression OT - lumbar spinal stenosis OT - randomised controlled trials COIS- All authors declare no competing interests. Funding: This research received no funding. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/26 CRDT- 2026/10/29 06:21 PHST- 2026/10/10 00:00 [received] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:21 [entrez] PHST- 2026/10/26 00:00 [pmc-release] AID - 172721 [pii] AID - 10.52965/001c.172721 [doi] PST - epublish SO - Orthop Rev (Pavia). 2026 Oct 26;18:172721. doi: 10.52965/001c.172721. eCollection 2026. PMID- 42953873 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1394-195X (Print) IS - 2180-4303 (Electronic) IS - 1394-195X (Linking) VI - 33 IP - 2 DP - 2026 Apr TI - Effects of Psychotherapy on Quality of Life among Patients with Inflammatory Bowel Disease: A Systematic Review with Meta-Analysis. PG - 8-23 LID - 10.21315/mjms-10-2025-706 [doi] AB - Individuals diagnosed with inflammatory bowel disease (IBD) have been known to have diminished quality of life (QoL). This present study aimed to examine the impact of psychotherapy on the QoL of patients with IBD. A systematic search for studies was conducted through a total of six databases, namely, MEDLINE, PubMed, Scopus, SAGE, ProQuest (psychology database), and ProQuest (public health database) from their date of inception up to 20 March 2024. The inclusion criteria were studies on patients with IBD, carried out through a randomised controlled trial design, published in the English language, and with full-text content available online. Two researchers independently extracted articles that met the selection criteria and evaluated the quality of each study. A random-effects meta-analysis was conducted, and data were presented in weighted mean difference (WMD) with a 95% CI. Fourteen studies were included in the systematic review, of which three were included in the meta-analysis. Overall, while psychotherapy showed no significant improvements in some QoL domains such as clinical bowel condition and systemic symptoms, notable enhancements were observed in social functioning and overall QoL. The findings underscore the importance of integrating psychotherapeutic approaches into IBD management to address psychological and social challenges, thereby enhancing overall patient well-being. These results have significant implications for clinical mental health counsellors and future research in IBD care. CI - (c) Penerbit Universiti Sains Malaysia, 2026. FAU - Subramaniam, Yashila AU - Subramaniam Y AD - School of Psychology, Faculty of Medical and Life Sciences, Sunway University, Bandar Sunway, Selangor, Malaysia. FAU - Ooi, Pei Boon AU - Ooi PB AD - School of Psychology, Faculty of Medical and Life Sciences, Sunway University, Bandar Sunway, Selangor, Malaysia. AD - Future Cities Research Institute, Sunway University, Bandar Sunway, Selangor, Malaysia. FAU - Hoo, Min Xi AU - Hoo MX AD - School of Psychology, Faculty of Medical and Life Sciences, Sunway University, Bandar Sunway, Selangor, Malaysia. FAU - Wong, Yin Yee AU - Wong YY AD - School of Psychology, Faculty of Medical and Life Sciences, Sunway University, Bandar Sunway, Selangor, Malaysia. FAU - Phang, Kelly AU - Phang K AD - Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. FAU - Ali, Raja Affendi Raja AU - Ali RAR AD - School of Psychology, Faculty of Medical and Life Sciences, Sunway University, Bandar Sunway, Selangor, Malaysia. FAU - Pickup, Roger AU - Pickup R AD - Faculty of Health and Medicine, Lancaster University, Lancaster, United Kingdom. LA - eng PT - Journal Article PT - Review DEP - 20260430 PL - Malaysia TA - Malays J Med Sci JT - The Malaysian journal of medical sciences : MJMS JID - 101126308 PMC - PMC13716331 OTO - NOTNLM OT - good health and well-being OT - inflammatory bowel disease OT - meta-analysis OT - psychotherapy OT - quality of life COIS- Conflict of Interest: None. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/04/01 CRDT- 2026/10/29 06:21 PHST- 2026/01/13 00:00 [received] PHST- 2026/03/26 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:21 [entrez] PHST- 2026/04/01 00:00 [pmc-release] AID - 02mjms3302_ra [pii] AID - 10.21315/mjms-10-2025-706 [doi] PST - ppublish SO - Malays J Med Sci. 2026 Apr;33(2):8-23. doi: 10.21315/mjms-10-2025-706. Epub 2026 Apr 30. PMID- 42953870 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2198-7793 (Print) IS - 2198-7793 (Electronic) IS - 2198-7793 (Linking) VI - 12 IP - 1 DP - 2026 TI - A Case of Radical Resection of a Mediastinal Invasive Parathyroid Tumor via a Thoracoscopic Approach. LID - 10.70352/scrj.cr.26-0266 [doi] LID - 26-0266 AB - INTRODUCTION: Parathyroid carcinoma is a rare malignancy that accounts for 0.3%-5.6% of cases of primary hyperparathyroidism (PHPT). Owing to the risk of tumor seeding, biopsy and fine-needle aspiration are contraindicated, posing a challenge to preoperative diagnosis of parathyroid carcinoma. Furthermore, complete tumor resection without capsular rupture is crucial because R1 resection substantially increases the risk of local recurrence. CASE PRESENTATION: A 71-year-old woman with hypercalcemia and renal dysfunction was referred to our hospital for further evaluation. Laboratory tests revealed a serum calcium level >12 mg/dL and markedly elevated intact parathyroid hormone (iPTH) levels. CT revealed diffuse skeletal sclerosis, leading to a diagnosis of PHPT caused by a parathyroid tumor. Imaging studies demonstrated that the tumor extended from the lower right neck into the superior mediastinum, with indistinct borders from the trachea and esophagus, raising concerns regarding malignancy and possible invasion. Therefore, a thoracoscopic approach was planned to achieve safe dissection and assess the feasibility of en bloc resection. The patient was initially placed in the left lateral decubitus position, and thoracoscopic mobilization of the mediastinal portion of the tumor was performed without capsular injury. After sufficient mobilization, the patient was moved to a supine position, and cervical dissection was completed through a small incision in the neck, achieving en bloc removal while preserving the recurrent laryngeal nerve. The patient developed pneumonia postoperatively, which resolved with conservative treatment. Pathological examination of the resected tumor revealed a parathyroid adenoma with capsular invasion and negative margins. The patient was discharged on POD 9 with normalized calcium and iPTH levels. CONCLUSIONS: The thoracoscopic approach may be a useful and safe strategy for treating parathyroid tumors that extend into the mediastinum, facilitating precise mobilization and en bloc resection without capsular rupture. Hence, a combined approach may enhance both the safety and curative potential of surgery in selected cases of parathyroid tumors with mediastinal extension. CI - (c) 2026 The Author(s). Published by Japan Surgical Society. FAU - Suzuki, Yusuke AU - Suzuki Y AD - Department of Digestive Surgery, Saitama Medical University International Medical Center, Hidaka, Saitama, Japan. FAU - Sato, Hiroshi AU - Sato H AD - Department of Digestive Surgery, Saitama Medical University International Medical Center, Hidaka, Saitama, Japan. FAU - Miyawaki, Yutaka AU - Miyawaki Y AD - Department of Digestive Surgery, Saitama Medical University International Medical Center, Hidaka, Saitama, Japan. FAU - Kodama, Ayumi AU - Kodama A AD - Department of Pathology, Saitama Medical University International Medical Center, Hidaka, Saitama, Japan. FAU - Yoshizawa, Masatoshi AU - Yoshizawa M AD - Department of Digestive Surgery, Saitama Medical University International Medical Center, Hidaka, Saitama, Japan. FAU - Furube, Tasuku AU - Furube T AD - Department of Digestive Surgery, Saitama Medical University International Medical Center, Hidaka, Saitama, Japan. FAU - Matsui, Kazuaki AU - Matsui K AD - Department of Digestive Surgery, Saitama Medical University International Medical Center, Hidaka, Saitama, Japan. FAU - Toriumi, Tetsuro AU - Toriumi T AD - Department of Digestive Surgery, Saitama Medical University International Medical Center, Hidaka, Saitama, Japan. FAU - Kishimoto, Yutaka AU - Kishimoto Y AD - Department of Digestive Surgery, Saitama Medical University International Medical Center, Hidaka, Saitama, Japan. FAU - Ebara, Gen AU - Ebara G AD - Department of Digestive Surgery, Saitama Medical University International Medical Center, Hidaka, Saitama, Japan. FAU - Sakuramoto, Shinichi AU - Sakuramoto S AD - Department of Digestive Surgery, Saitama Medical University International Medical Center, Hidaka, Saitama, Japan. LA - eng PT - Case Reports PT - Journal Article DEP - 20261024 PL - Japan TA - Surg Case Rep JT - Surgical case reports JID - 101662125 PMC - PMC13716533 OTO - NOTNLM OT - parathyroid tumor OT - progression within the mediastinum OT - thoracoscopic surgery COIS- The authors declare that they have no competing interests. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/24 CRDT- 2026/10/29 06:21 PHST- 2026/06/03 00:00 [received] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:21 [entrez] PHST- 2026/10/24 00:00 [pmc-release] AID - scrj.cr.26-0266 [pii] AID - 10.70352/scrj.cr.26-0266 [doi] PST - ppublish SO - Surg Case Rep. 2026;12(1):26-0266. doi: 10.70352/scrj.cr.26-0266. Epub 2026 Oct 24. PMID- 42953867 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 0973-6247 (Print) IS - 1998-3565 (Electronic) IS - 0973-6247 (Linking) VI - 20 IP - 2 DP - 2026 Jul-Dec TI - Unraveling the mystery of e variant: Is it autoimmune or alloimmune? PG - 464-468 LID - 10.4103/ajts.ajts_54_25 [doi] AB - The largest and most diverse blood group system is the Rh system. Numerous RH alleles lead to variable morphologies, which frequently make it tougher to differentiate between auto- and allo-antibodies in recipients with anti-Rh antibodies when their own corresponding Rh antigen is present. We present a noteworthy case involving a 45-year-old female patient who tested positive on the antibody screen. The patient exhibited inconsistent reactions during antibody identification, suggesting the potential presence of either an autoantibody or an alloantibody targeting the RHCE variant. Double alloadsorption, use of select cells, and consideration of ethnic background aided in the correct identification of allo anti-e in e variant case. This case highlights the clinical relevance of RHCE variants, particularly in patients of African descent, where partial e antigen expression is more prevalent and can result in serologic discrepancies and alloimmunization. CI - Copyright: (c) 2026 Asian Journal of Transfusion Science. FAU - Dogra, Mitu AU - Dogra M AD - Department of Transfusion Medicine, BLK-Max Super Speciality Hospital, Delhi, India. FAU - Khaliq, Vadad AU - Khaliq V AD - Department of Transfusion Medicine, BLK-Max Super Speciality Hospital, Delhi, India. FAU - Setia, Rasika Dhawan AU - Setia RD AD - Department of Transfusion Medicine, BLK-Max Super Speciality Hospital, Delhi, India. FAU - Rahman, Amena Ebadur AU - Rahman AE AD - Department of Transfusion Medicine, BLK-Max Super Speciality Hospital, Delhi, India. LA - eng PT - Case Reports PT - Journal Article DEP - 20260204 PL - India TA - Asian J Transfus Sci JT - Asian journal of transfusion science JID - 101306858 PMC - PMC13716274 OTO - NOTNLM OT - Alloadsorptions OT - Rh altered forms OT - alloimmune OT - autoimmune OT - e-variant OT - ethnic group COIS- There are no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/07/01 CRDT- 2026/10/29 06:20 PHST- 2025/03/24 00:00 [received] PHST- 2025/07/03 00:00 [revised] PHST- 2025/08/11 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:20 [entrez] PHST- 2026/07/01 00:00 [pmc-release] AID - AJTS-20-464 [pii] AID - 10.4103/ajts.ajts_54_25 [doi] PST - ppublish SO - Asian J Transfus Sci. 2026 Jul-Dec;20(2):464-468. doi: 10.4103/ajts.ajts_54_25. Epub 2026 Feb 4. PMID- 42953865 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 0973-6247 (Print) IS - 1998-3565 (Electronic) IS - 0973-6247 (Linking) VI - 20 IP - 2 DP - 2026 Jul-Dec TI - Adsorption-elution: Revisiting an age-old technique for identifying multiple alloantibodies and ensuring safe transfusion in a pregnant patient with HbE-beta-thalassemia. PG - 431-434 LID - 10.4103/ajts.ajts_178_24 [doi] AB - Identification of multiple alloantibodies requires an extensive array of immunohematology reagents, which is time-consuming. Following antibody identification (AI), providing corresponding antigen-negative red cell transfusion is paramount, which remains challenging in resource-constraint centers. Here, we report the case of a 34-year-old pregnant patient with HbE-beta-thalassemia who presented with severe anemia requiring lifesaving transfusion support. During pretransfusion testing, an incompatible crossmatch, mandated antibody screening (AS) and AI. AS was pan-positive with differential strength and autocontrol was negative, suggesting multiple alloantibodies. Initial AI results were ambiguous and unresolved due to a lack of select cells and extended panels: a common scenario in most blood centers. Here, a simple adsorption-elution method was performed with incompatible red cells to identify the antibodies. Anti-E was identified in the eluate. Anti-Fy(b) and anti-Jk(a) were detected in the adsorbed serum. With only Rh antisera available, the presence of Anti-E was confirmed. However, the absence of other minor antisera posed challenges in ensuring a safe transfusion. A simple adsorption-elution study with compatible red cells was then performed to confirm the absence of single-dose antigens, which may not be detected by standard AHG crossmatching. This case highlights that a simplified algorithmic approach for adsorption-elution studies can be adopted for providing the best suitable red cell transfusion support in resource-limited centers. CI - Copyright: (c) 2025 Asian Journal of Transfusion Science. FAU - Deb, Joyisa AU - Deb J AD - Department of Transfusion Medicine and Blood Centre, All India Institute of Medical Sciences, Guwahati, Assam, India. FAU - Gogoi, Priyanka AU - Gogoi P AD - Department of Transfusion Medicine and Blood Centre, All India Institute of Medical Sciences, Guwahati, Assam, India. FAU - Ray, Gopal Krushna AU - Ray GK AD - Department of Transfusion Medicine and Blood Centre, All India Institute of Medical Sciences, Guwahati, Assam, India. FAU - Hoque, Samim Sultana AU - Hoque SS AD - Department of Transfusion Medicine and Blood Centre, All India Institute of Medical Sciences, Guwahati, Assam, India. LA - eng PT - Case Reports PT - Journal Article DEP - 20250722 PL - India TA - Asian J Transfus Sci JT - Asian journal of transfusion science JID - 101306858 PMC - PMC13716281 OTO - NOTNLM OT - Adsorption-elution studies OT - alloimmunization OT - multiple alloantibodies detection OT - resource-constraint setting COIS- There are no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/07/01 CRDT- 2026/10/29 06:20 PHST- 2024/10/28 00:00 [received] PHST- 2025/01/01 00:00 [revised] PHST- 2025/01/26 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:20 [entrez] PHST- 2026/07/01 00:00 [pmc-release] AID - AJTS-20-431 [pii] AID - 10.4103/ajts.ajts_178_24 [doi] PST - ppublish SO - Asian J Transfus Sci. 2026 Jul-Dec;20(2):431-434. doi: 10.4103/ajts.ajts_178_24. Epub 2025 Jul 22. PMID- 42953864 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1394-195X (Print) IS - 2180-4303 (Electronic) IS - 1394-195X (Linking) VI - 33 IP - 2 DP - 2026 Apr TI - Foley Catheter-Assisted Evacuation of Clot Versus Conventional Craniotomy for Spontaneous Supratentorial Intracerebral Haemorrhage: A Single-Centre Observational Study. PG - 123-134 LID - 10.21315/mjms-11-2025-778 [doi] AB - BACKGROUND: Minimally invasive surgery (MIS) is increasingly recommended for spontaneous intracerebral haemorrhage (ICH). This study aimed to compare outcomes between patients undergoing Foley catheter-assisted MIS and those treated by conventional craniotomy in a real-world clinical setting. METHODS: A single-centre, dual-cohort observational study was conducted. A prospective cohort of consecutive patients (n = 35) who underwent Foley catheter-assisted evacuation was compared with a retrospective historical cohort (n = 35) treated with conventional craniotomy. Eligible patients were adults (18 to 75 years old) with supratentorial ICH volumes >/= 30 mL and an admission Glasgow Coma Scale (GCS) score of 6-12. Primary outcomes included intraoperative blood loss, surgery duration, and the Glasgow Outcome Scale (GOS) score at 3 months. Secondary outcomes encompassed seizure incidence, rebleeding rates, and Mini-Mental State Examination (MMSE) scores at 1 month postoperatively. RESULTS: Compared with the conventional craniotomy cohort, the Foley catheter cohort was associated with significantly lower intraoperative blood loss (120 +/- 30 mL vs. 210 +/- 45 mL, P = 0.042), a shorter surgery duration (150 +/- 25 min vs. 195 +/- 35 min, P = 0.001), and a better GOS score at 3 months (4.1 +/- 0.8 vs. 2.9 +/- 0.6, P < 0.001). The incidence of postoperative seizures was also lower in the Foley catheter group (5.7% vs. 25.7%, P = 0.021). Rebleeding rates did not differ significantly between the cohorts (11.4% vs. 20.0%, P = 0.324). The MMSE scores at 1 month were higher in the Foley catheter cohort (P < 0.001). CONCLUSION: In this observational cohort study, Foley catheter-assisted evacuation was associated with reduced operative time, less intraoperative blood loss, a lower incidence of seizures, and better early functional and cognitive outcomes compared with conventional craniotomy. These findings support the potential utility of this technique and warrant further investigation in larger, prospective studies to establish causal efficacy. CI - (c) Penerbit Universiti Sains Malaysia, 2026. FAU - Arumugam, Ananda AU - Arumugam A AD - Faculty of Medicine, Lincoln University College, Petaling Jaya, Selangor, Malaysia. AD - Department of Neurosurgery, Hospital Queen Elizabeth, Kota Kinabalu, Sabah, Malaysia. FAU - Vinayaga, Sakti AU - Vinayaga S AD - Department of Neurosurgery, Hospital Queen Elizabeth, Kota Kinabalu, Sabah, Malaysia. FAU - Cheah, Pike See AU - Cheah PS AD - Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Seri Kembangan, Selangor, Malaysia. FAU - Abdelwahab, Emad Mohamad Nafie AU - Abdelwahab EMN AD - Faculty of Medicine, Lincoln University College, Petaling Jaya, Selangor, Malaysia. LA - eng PT - Journal Article DEP - 20260430 PL - Malaysia TA - Malays J Med Sci JT - The Malaysian journal of medical sciences : MJMS JID - 101126308 PMC - PMC13716321 OTO - NOTNLM OT - Foley catheter technique OT - basal ganglia haemorrhage OT - intraparenchymal bleed OT - minimally invasive evacuation OT - spontaneous intracerebral haemorrhage COIS- Conflict of Interest: None. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/04/01 CRDT- 2026/10/29 06:20 PHST- 2025/11/13 00:00 [received] PHST- 2026/03/06 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:20 [entrez] PHST- 2026/04/01 00:00 [pmc-release] AID - 10mjms3302_oa [pii] AID - 10.21315/mjms-11-2025-778 [doi] PST - ppublish SO - Malays J Med Sci. 2026 Apr;33(2):123-134. doi: 10.21315/mjms-11-2025-778. Epub 2026 Apr 30. PMID- 42953837 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 0973-6247 (Print) IS - 1998-3565 (Electronic) IS - 0973-6247 (Linking) VI - 20 IP - 2 DP - 2026 Jul-Dec TI - Assessment of factor-VIII levels in thawed fresh frozen plasma stored at 2 degrees C-6 degrees C for 5 days. PG - 295-300 LID - 10.4103/ajts.ajts_138_23 [doi] AB - BACKGROUND: Plasma is the cell-free part of blood prepared either from whole blood or by plasmapheresis. Fresh frozen plasma (FFP) must be stored at -18 degrees C or colder and has a shelf life of 12 months. In routine practice, after thawing, FFP is stored for 24 h at 1 degrees C-6 degrees C if not transfused, and subsequently, it is discarded, leading to the wastage of valuable resources. This study measured the changes of coagulation factor VIII (FVIII) activities over 5 days in thawed FFP. AIMS: The objective of the study was to assess FVIII levels in thawed FFP immediately after thawing and after storage at 2 degrees C-6 degrees C for 24 h, 72 h, and 120 h and thereby to assess whether thawed FFP stored for 5 days can be considered to ameliorate coagulation factor deficiencies in affected patients. MATERIALS AND METHODS: 100 blood donors, 25 each from A, B, AB, and O blood groups were randomly selected. Coagulation test parameters, prothrombin time, activated partial thromboplastin time, and FVIII:C, were measured at 0, 24, 72, and 120 h using an ACL ELITE PRO coagulometer (Instrumentation Laboratory, USA). Measurements of coagulation factors were conducted using one-stage clotting factor assays. Data were analyzed statistically using analysis of variance and paired t-test. RESULTS: The average FVIII activity immediately after thawing (0 h) was 137.1%. FVIII activities were reduced significantly (P < 0.05) from 0 h to 120 h of storage at 2 degrees C-6 degrees C. A maximum decrease was seen in the first 24 h after thawing. The average FVIII activity at 120 h was 66.75%. CONCLUSIONS: Coagulation FVIII activities in thawed FFP on Day 5 were still sufficient to treat bleeding patients as the activities were more than 30%. Therefore, thawed FFP stored for 5 days can be considered for use in needy patients in emergency cases as it can ensure immediate availability as well as the reduction in wastage. However, further research and clinical trials are necessary to confirm these findings. CI - Copyright: (c) 2025 Asian Journal of Transfusion Science. FAU - Mathew, Ashly Monson AU - Mathew AM AD - Department of Transfusion Medicine, Government Medical College, Thrissur, Kerala, India. FAU - Shah, Sangita D AU - Shah SD AD - Department of IHBT, B.J. Medical College, Ahmedabad, Gujarat, India. FAU - Bhatnagar, Nidhi AU - Bhatnagar N AD - Department of IHBT, B.J. Medical College, Ahmedabad, Gujarat, India. FAU - Shah, Mamta AU - Shah M AD - Department of IHBT, B.J. Medical College, Ahmedabad, Gujarat, India. FAU - Patel, Tarak AU - Patel T AD - Department of IHBT, U.N. Mehta Institute of Cardiolgy and Research, Ahmedabad, Gujarat, India. LA - eng PT - Journal Article DEP - 20250124 PL - India TA - Asian J Transfus Sci JT - Asian journal of transfusion science JID - 101306858 PMC - PMC13716247 OTO - NOTNLM OT - Factor VIII OT - plasma OT - thawed fresh frozen plasma COIS- There are no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/07/01 CRDT- 2026/10/29 06:19 PHST- 2023/07/12 00:00 [received] PHST- 2024/05/06 00:00 [revised] PHST- 2024/10/13 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:19 [entrez] PHST- 2026/07/01 00:00 [pmc-release] AID - AJTS-20-295 [pii] AID - 10.4103/ajts.ajts_138_23 [doi] PST - ppublish SO - Asian J Transfus Sci. 2026 Jul-Dec;20(2):295-300. doi: 10.4103/ajts.ajts_138_23. Epub 2025 Jan 24. PMID- 42953820 OWN - NLM STAT- Publisher LR - 20261029 IS - 2046-2069 (Electronic) IS - 2046-2069 (Linking) DP - 2026 Oct 28 TI - Carbon dot/TiO(2) nanocomposites for photocatalytic antibiotic degradation: interfacial engineering, degradation mechanisms, and environmental remediation. LID - 10.1039/d6ra08578d [doi] AB - The widespread presence of antibiotic residues in aquatic environments contributes to ecological toxicity and accelerates antimicrobial resistance (AMR), highlighting the need for efficient and sustainable remediation technologies. Among advanced oxidation processes, TiO(2)-based photocatalysis has attracted considerable attention because of its chemical stability, low toxicity, and strong oxidative capability. However, the practical application of TiO(2) is limited by poor visible-light absorption and rapid electron-hole recombination. Carbon dots (CDs) have emerged as highly promising interfacial modifiers owing to their tunable electronic structure, visible-light responsiveness, and efficient charge-transfer capability, enabling substantial enhancement of TiO(2) photocatalytic performance. This review critically examines recent advances in CDs/TiO(2) nanocomposites for photocatalytic antibiotic degradation, with particular emphasis on how heterointerface engineering governs charge-carrier dynamics, reactive oxygen species (ROS) generation, and degradation selectivity. The mechanistic roles of Ti-O-C interfacial bonding, band alignment, defect-state modulation, and interfacial electron transfer are systematically discussed in relation to photocatalytic activity across major antibiotic classes, including tetracyclines, fluoroquinolones, beta-lactams, and sulfonamides. In addition, the effects of adsorption behavior, operational parameters, environmental matrix components, and reactor configuration on degradation efficiency and mineralization are critically evaluated. Special attention is devoted to degradation pathways, catalyst photostability, ecotoxicological considerations, and challenges associated with real-water applications and large-scale implementation. Current limitations, including insufficient mechanistic validation, lack of standardized benchmarking protocols, and limited long-term environmental safety assessment, are also highlighted. Finally, current research priorities involving mechanistic validation, predictive interfacial engineering, standardized benchmarking, environmental safety assessment, and solar-driven photocatalytic design are highlighted to guide the future development of sustainable antibiotic-remediation technologies. CI - This journal is (c) The Royal Society of Chemistry. FAU - Mohammed, Sewara J AU - Mohammed SJ AUID- ORCID: 0000-0003-3257-6242 AD - Department of Chemistry, College of Science, University of Sulaimani Qlyasan Street Sulaymaniyah 46001 Kurdistan Regional Government Iraq sewara.mohammed@univsul.edu.iq. FAU - Hamarashid, Eman O AU - Hamarashid EO AD - Department of Chemistry, College of Science, University of Sulaimani Qlyasan Street Sulaymaniyah 46001 Kurdistan Regional Government Iraq sewara.mohammed@univsul.edu.iq. FAU - Najmuldeen, Hastyar H AU - Najmuldeen HH AUID- ORCID: 0000-0002-7710-6845 AD - Department of Medical Laboratory Analysis, College of Health Sciences, Cihan University Sulaimaniya Sulaymaniyah City Kurdistan Iraq. AD - Department of Biology, College of Science, University of Sulaimani Qlyasan Street Sulaymaniyah 46001 Kurdistan Regional Government Iraq. FAU - Kader, Dana A AU - Kader DA AUID- ORCID: 0000-0002-5705-5868 AD - Department of Chemistry, College of Education, University of Sulaimani Old Campus Sulaymaniyah 46001 Kurdistan Regional Government Iraq. FAU - Sidiq, Mohammed K AU - Sidiq MK AUID- ORCID: 0009-0007-9181-3523 AD - Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy, University of Sulaimani Sulaymaniyah Kurdistan Regional Government 46001 Iraq. FAU - Hussain, Faiq H S AU - Hussain FHS AUID- ORCID: 0000-0003-1987-8422 AD - Medical Analysis Department, Faculty of Applied Science, Tishk International University Erbil Kurdistan Region 44001 Iraq. FAU - Mohammed, Awat S AU - Mohammed AS AD - Department of Chemistry, College of Science, University of Sulaimani Qlyasan Street Sulaymaniyah 46001 Kurdistan Regional Government Iraq sewara.mohammed@univsul.edu.iq. FAU - Alghofaili, Fayez AU - Alghofaili F AD - Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University Majmaah 11952 Saudi Arabia. FAU - Hamasalih, Govar H AU - Hamasalih GH AD - Department of Physics, College of Education, University of Sulaimani, Kurdistan Regional Government Old Campus Sulaymaniyah 46001 Iraq. FAU - Mhamad, Shakhawan A AU - Mhamad SA AD - Department of Chemistry, College of Education, University of Sulaimani Old Campus Sulaymaniyah 46001 Kurdistan Regional Government Iraq. FAU - Faqi-Khedr, Yar M AU - Faqi-Khedr YM AUID- ORCID: 0009-0004-4641-4599 AD - Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy, University of Sulaimani Sulaymaniyah Kurdistan Regional Government 46001 Iraq. FAU - Kayani, Kawan F AU - Kayani KF AUID- ORCID: 0000-0003-1159-2147 AD - Department of Chemistry, College of Science, University of Sulaimani Qlyasan Street Sulaymaniyah 46001 Kurdistan Regional Government Iraq sewara.mohammed@univsul.edu.iq. FAU - Hamaamin, Othman K AU - Hamaamin OK AD - Department of Chemistry, College of Science, University of Sulaimani Qlyasan Street Sulaymaniyah 46001 Kurdistan Regional Government Iraq sewara.mohammed@univsul.edu.iq. FAU - Aziz, Shujahadeen B AU - Aziz SB AD - Turning Trash to Treasure Laboratory (TTTL), Research and Development Center, University of Sulaimani Qlyasan Street Sulaymaniyah Kurdistan Regional Government 46001 Iraq. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - RSC Adv JT - RSC advances JID - 101581657 PMC - PMC13715865 COIS- There are no conflicts to declare. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 PMCR- 2026/10/28 CRDT- 2026/10/29 06:18 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:18 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - d6ra08578d [pii] AID - 10.1039/d6ra08578d [doi] PST - aheadofprint SO - RSC Adv. 2026 Oct 28. doi: 10.1039/d6ra08578d. PMID- 42953814 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1178-2013 (Electronic) IS - 1176-9114 (Print) IS - 1176-9114 (Linking) VI - 21 DP - 2026 TI - A Narrative Review of Nanomedicine in Cancer Therapy: Modulating Metabolic Reprogramming to Enhance Immunotherapy. PG - 650413 LID - 10.2147/IJN.S650413 [doi] LID - 650413 AB - BACKGROUND: Metabolic reprogramming of tumor cells interacts with functional alterations of immune cells to establish an immunosuppressive tumor microenvironment, which constrains the efficacy of immunotherapy. Nanomedicine provides technical approaches for targeted delivery and controlled release of metabolic modulators. MAIN TEXT: This review outlines major nanocarriers and their design principles, dissects the crosstalk among glucose, amino acid and lipid metabolism, and illustrates how nutrient competition and metabolic byproducts shape antitumor immune responses. It focuses on summarizing advances in nanodelivery strategies targeting these metabolic pathways and their combinatorial application with immunotherapy. CONCLUSION: Nanomedicine-mediated metabolic intervention holds promise for remodeling the tumor immune microenvironment. However, tumor cells and effector T cells share partial metabolic requirements, and nonselective inhibition may impair antitumor immunity. Improving the cell selectivity of interventions and validating long-term safety and clinical benefits represent key considerations for future translational research. CI - (c) 2026 Wu et al. FAU - Wu, Hongpeng AU - Wu H AD - Department of Thyroid Surgery, The First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China. FAU - Wang, Fengyi AU - Wang F AD - Department of Gynecology, The First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China. FAU - Sun, Wei AU - Sun W AD - Department of Thyroid Surgery, The First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China. FAU - Zhang, Hao AU - Zhang H AUID- ORCID: 0000-0002-9938-8433 AD - Department of Thyroid Surgery, The First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China. LA - eng PT - Journal Article PT - Review DEP - 20261024 PL - New Zealand TA - Int J Nanomedicine JT - International journal of nanomedicine JID - 101263847 SB - IM MH - Humans MH - Metabolic Reprogramming MH - *Immunotherapy/methods MH - *Neoplasms/therapy/metabolism/immunology MH - *Nanomedicine/methods MH - Animals MH - Tumor Microenvironment/drug effects/immunology PMC - PMC13715856 OTO - NOTNLM OT - cancer therapy OT - immunotherapy OT - metabolic reprogramming OT - nanomedicine COIS- The authors declare that they have no competing interests. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/24 CRDT- 2026/10/29 06:18 PHST- 2026/08/21 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:18 [entrez] PHST- 2026/10/24 00:00 [pmc-release] AID - 650413 [pii] AID - 10.2147/IJN.S650413 [doi] PST - epublish SO - Int J Nanomedicine. 2026 Oct 24;21:650413. doi: 10.2147/IJN.S650413. eCollection 2026. PMID- 42953810 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2168-8184 (Print) IS - 2168-8184 (Electronic) IS - 2168-8184 (Linking) VI - 18 IP - 10 DP - 2026 Oct TI - Diffusion-Weighted MRI of Breast Lesions: A Five-Case Series Demonstrating Imaging Patterns and Diagnostic Pitfalls. PG - e118733 LID - 10.7759/cureus.118733 [doi] LID - e118733 AB - Diffusion-weighted imaging (DWI) is an adjunct MRI sequence that helps characterize breast lesions by providing valuable information about tissue microstructure based on the in vivo motion of water molecules. Malignant lesions typically restrict the free diffusion of water molecules, appearing bright on high b-value images and dark (with a low apparent diffusion coefficient (ADC)) on the ADC map. Some benign lesions can show similar DWI findings, leading to diagnostic challenges when DWI is used as an independent sequence for lesion characterization. We report five breast MRI cases demonstrating varied diffusion patterns and the pitfalls encountered during interpretation. The cases include invasive ductal carcinoma with regional axillary nodal disease, metaplastic carcinoma, fibroadenoma without true diffusion restriction, fibroadenoma with true diffusion restriction, and a post-procedural seroma. Both malignant lesions showed restricted diffusion. Among the two pathologically proven fibroadenomas, one showed T2 shine-through, whereas the other demonstrated true diffusion restriction. The post-procedural lesion showed facilitated diffusion despite thin rim post-contrast enhancement, suggestive of a seroma. These cases demonstrate the overlap in diffusion findings between benign and malignant breast lesions, emphasizing the importance of interpreting diffusion images in conjunction with ADC maps, lesion morphology, enhancement patterns, and clinical history. CI - Copyright (c) 2026, Yadav et al. FAU - Yadav, Preeti AU - Yadav P AD - Radiology, World College of Medical Sciences, Jhajjar, IND. FAU - Mukherjee, Arindam AU - Mukherjee A AD - Radiology, BLK Super Speciality Hospital, New Delhi, IND. FAU - Samanta, Riya AU - Samanta R AD - Radiodiagnosis, Lady Hardinge Medical College, New Delhi, IND. LA - eng PT - Case Reports PT - Journal Article DEP - 20261028 PL - United States TA - Cureus JT - Cureus JID - 101596737 PMC - PMC13716140 OTO - NOTNLM OT - apparent diffusion coefficient OT - breast lesions OT - breast mri OT - diffusion restriction OT - diffusion-weighted imaging OT - fibroadenoma OT - seroma COIS- Human subjects: Informed consent for treatment and open access publication was obtained or waived by all participants in this study. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/28 CRDT- 2026/10/29 06:17 PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:17 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 10.7759/cureus.118733 [doi] PST - epublish SO - Cureus. 2026 Oct 28;18(10):e118733. doi: 10.7759/cureus.118733. eCollection 2026 Oct. PMID- 42953787 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1179-142X (Electronic) IS - 1179-142X (Linking) VI - 19 DP - 2026 TI - Posterior Realignment and Anterior Column Reconstruction of Thoracic Spondyloptosis Using Intraoperative CT and Navigation: Case Report and Review of the Literature. PG - 653615 LID - 10.2147/IMCRJ.S653615 [doi] LID - 653615 AB - INTRODUCTION: Traumatic thoracic spondyloptosis is an exceptionally unstable three-column injury, usually caused by high-energy trauma and frequently associated with severe spinal cord injury. Reduction and reconstruction are technically demanding because normal anatomical relationships are lost. CASE REPORT: An 18-year-old man presented after a motorcycle collision with complete paraplegia and a T4 sensory level, consistent with AIS A spinal cord injury. CT demonstrated T5-T6 spondyloptosis with translation, rotation, vertebral comminution, and complete spinal-column disruption; MRI showed severe cord distortion, T2 hyperintensity, and an epidural hematoma. Emergency single-stage posterior surgery included CT-based neuronavigated pedicle screw placement, T6 laminectomy and corpectomy, T5 decompression, epidural hematoma evacuation, progressive realignment, and anterior-column reconstruction with an interbody cage. Repeated intraoperative CT acquisitions were used to reassess the changing anatomy and verify instrumentation, cage position, and final alignment. Postoperative imaging confirmed satisfactory reconstruction. No perioperative complication occurred. At 5-year follow-up, neurological status remained unchanged. CONCLUSION: In selected patients with severe mid-thoracic spondyloptosis, a posterior-only strategy can permit decompression, realignment, anterior-column reconstruction, and definitive stabilization in one procedure. Iterative intraoperative CT-based navigation may be particularly useful when anatomy changes substantially during reconstruction, although comparative data are lacking. CI - (c) 2026 Zaed et al. FAU - Zaed, Ismail AU - Zaed I AUID- ORCID: 0000-0003-3506-4111 AD - Department of Neurosurgery Neurocenter of South Switzerland, EOC, Lugano, Switzerland. AD - Neurosurgical Outcomes Research & Analytics (NORA) Laboratory, Lugano, Switzerland. FAU - Kuhlen, Dominique E AU - Kuhlen DE AD - Department of Neurosurgery Neurocenter of South Switzerland, EOC, Lugano, Switzerland. FAU - Marchi, Francesco AU - Marchi F AD - Department of Neurosurgery Neurocenter of South Switzerland, EOC, Lugano, Switzerland. AD - Neurosurgical Outcomes Research & Analytics (NORA) Laboratory, Lugano, Switzerland. LA - eng PT - Case Reports PT - Journal Article PT - Review DEP - 20261024 PL - New Zealand TA - Int Med Case Rep J JT - International medical case reports journal JID - 101566269 SB - IM MH - Humans MH - Male MH - Adolescent MH - *Thoracic Vertebrae/surgery/injuries/diagnostic imaging MH - *Spondylolisthesis/surgery/diagnostic imaging MH - Tomography, X-Ray Computed MH - Accidents, Traffic MH - *Neuronavigation/methods MH - *Plastic Surgery Procedures/methods MH - *Spinal Cord Injuries/surgery/diagnostic imaging MH - Laminectomy/methods MH - Decompression, Surgical/methods MH - Magnetic Resonance Imaging PMC - PMC13715847 OAB - Spondyloptosis is a rare traumatic injury in which one spinal segment becomes completely displaced from the next. We report an 18-year-old man with a severe T5-T6 injury after a motorcycle collision and complete paraplegia. Surgery was performed entirely from the back. Intraoperative CT scans and navigation were used repeatedly to guide screw placement and to check the reconstruction while the spine was being realigned. A cage was placed to rebuild the front part of the spinal column. The spine was successfully realigned and stabilized without perioperative complications. Neurological function did not recover over long-term follow-up, reflecting the severity of the initial spinal cord injury. OABL- eng OTO - NOTNLM OT - corpectomy OT - intraoperative CT OT - neuronavigation OT - spinal reconstruction OT - spinal trauma OT - thoracic spondyloptosis COIS- The authors report no conflicts of interest in this work. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/24 CRDT- 2026/10/29 06:16 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:16 [entrez] PHST- 2026/10/24 00:00 [pmc-release] AID - 653615 [pii] AID - 10.2147/IMCRJ.S653615 [doi] PST - epublish SO - Int Med Case Rep J. 2026 Oct 24;19:653615. doi: 10.2147/IMCRJ.S653615. eCollection 2026. PMID- 42953783 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1179-1411 (Print) IS - 1179-1411 (Electronic) IS - 1179-1411 (Linking) VI - 18 DP - 2026 TI - Threatened Miscarriage: Evidence-Based Status and Advances in Western Medical Treatments. PG - 638716 LID - 10.2147/IJWH.S638716 [doi] LID - 638716 AB - Threatened miscarriage is one of the most common complications during pregnancy and is characterized by complex and highly heterogeneous etiologies. In recent years, with the emergence of high-quality randomized controlled trials and updates in evidence-based guidelines, the management of threatened miscarriage has gradually shifted from empirical pregnancy preservation to risk stratification and precision intervention. This review summarizes the current evidence base and recent advances in Western medical treatment for threatened miscarriage, based on the latest clinical guidelines, randomized controlled trials, systematic reviews, and meta-analyses. Particular emphasis is placed on progesterone therapy, antithrombotic treatment, hormonal regulation during pregnancy, immunomodulatory therapy, tocolysis, and nutritional support. Current evidence indicates that vaginal micronized progesterone provides clear benefits for women with early pregnancy bleeding and a history of miscarriage. In contrast, the use of low-molecular-weight heparin (LMWH) and low-dose aspirin (LDA) should be strictly restricted to selected high-risk populations. Levothyroxine (LT4) and metformin may offer potential benefits in patients with specific endocrine abnormalities; however, evidence supporting their routine use remains limited. In addition, the long-term benefits of immunomodulatory and tocolytic therapies remain uncertain. Overall, the Western medical management of threatened miscarriage should be individualized based on underlying etiological mechanisms, clinical phenotypes, and evidence-based recommendations. Future studies integrating biomarkers and multi-omics approaches are warranted to advance precision phenotyping and personalized treatment strategies for threatened miscarriage. CI - (c) 2026 Wang et al. FAU - Wang, Yu AU - Wang Y AD - Department of Gynecology, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, 150000, People's Republic of China. FAU - Gao, Zhuwei AU - Gao Z AUID- ORCID: 0009-0004-7188-0460 AD - Graduate School, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, 150000, People's Republic of China. FAU - Yao, Yu AU - Yao Y AD - Graduate School, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, 150000, People's Republic of China. FAU - Wu, You AU - Wu Y AD - Graduate School, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, 150000, People's Republic of China. FAU - Jiang, Shan AU - Jiang S AD - Graduate School, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, 150000, People's Republic of China. FAU - Duan, Yuqi AU - Duan Y AD - Graduate School, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, 150000, People's Republic of China. FAU - Zhao, Jiahui AU - Zhao J AD - Graduate School, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, 150000, People's Republic of China. FAU - Wu, Xiaoke AU - Wu X AD - Department of Gynecology, First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang, 150000, People's Republic of China. AD - The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, Zhejiang, 310053, People's Republic of China. FAU - Gao, Jingshu AU - Gao J AD - The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, Zhejiang, 310053, People's Republic of China. LA - eng PT - Journal Article PT - Review DEP - 20261024 PL - New Zealand TA - Int J Womens Health JT - International journal of women's health JID - 101531698 PMC - PMC13715843 OTO - NOTNLM OT - anticoagulants OT - pregnancy maintenance OT - progesterone OT - risk stratification OT - threatened abortion OT - threatened miscarriage COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/24 CRDT- 2026/10/29 06:16 PHST- 2026/07/07 00:00 [received] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:16 [entrez] PHST- 2026/10/24 00:00 [pmc-release] AID - 638716 [pii] AID - 10.2147/IJWH.S638716 [doi] PST - epublish SO - Int J Womens Health. 2026 Oct 24;18:638716. doi: 10.2147/IJWH.S638716. eCollection 2026. PMID- 42953776 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1177-8881 (Electronic) IS - 1177-8881 (Linking) VI - 20 DP - 2026 TI - Efficacy and Safety of Moderate-Dose Glucocorticoids with Tacrolimus in High-Risk Membranous Nephropathy: A Real-World Comparison of Three Immunosuppressive Regimens. PG - 639867 LID - 10.2147/DDDT.S639867 [doi] LID - 639867 AB - OBJECTIVE: This study was designed to evaluate the efficacy of moderate-dose glucocorticoid (mGC) combined with tacrolimus (FK506) in high-risk patients with membranous nephropathy (MN), and compare with two widely used therapies: full-dose glucocorticoid (GC) plus cyclophosphamide (CTX), and rituximab (RTX) plus FK506, the latter being recommended by the Kidney Disease: Improving Global Outcomes (KDIGO) Clinical Practice Guidelines. This study aims to provide evidence for individualized treatment in high-risk MN. METHODS: A total of 154 patients with high-risk MN were enrolled and assigned into three treatment groups: full-dose GC combined with CTX group (GC+CTX) (n=53), mGC combined with FK506 group (mGC+FK506) (n=51), and RTX combined with FK506 group (RTX+FK506) (n=50). Baseline clinical and laboratory parameters and follow-up data at 1, 3, 6, and 12 months post-treatment were collected. Outcomes including disease remission rates, relapse rates, and adverse events were compared among the three groups. RESULTS: At 12 months of follow-up, the overall remission rates for the GC+CTX, mGC+FK506, and RTX+FK506 groups were 94.4%, 94.1%, and 82.0%, respectively, with corresponding complete remission rates of 32.1%, 43.1%, and 24.0%. No significant intergroup differences were observed (P > 0.05). The mGC+FK506 group demonstrated higher remission rates at both 3 and 6 months. Multivariate Cox regression indicated that baseline serum albumin > 20 g/L was a protective factor (HR = 1.638, P = 0.004), and comorbid diabetes mellitus an independent risk factor (HR = 0.617, P = 0.032). CONCLUSION: The mGC+FK506 regimen was non-inferior to the GC+CTX and RTX+FK506 regimens in treating high-risk MN, with an earlier onset of therapeutic effect and superior remission rates at 3 and 6 months. Early correction of hypoalbuminemia and strict glycemic control are critical for improving prognosis. This study may provide evidence for individualized treatment selection in high-risk MN patients who are intolerant to high-dose GC or have limited access to RTX. CI - (c) 2026 Sui et al. FAU - Sui, Lu AU - Sui L AUID- ORCID: 0009-0002-1707-2302 AD - Department of Nephrology, the First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China. FAU - Ma, Yutong AU - Ma Y AD - Department of Nephrology, the First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China. FAU - Yu, Xintian AU - Yu X AUID- ORCID: 0009-0007-1221-6617 AD - Department of Integrated Traditional Chinese and Western Medicine, Liaoning University of Traditional Chinese Medicine Xinglin College, Shenyang, Liaoning, People's Republic of China. FAU - Meng, Yilin AU - Meng Y AUID- ORCID: 0009-0003-9468-3956 AD - Department of Nephrology, the First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China. FAU - Cui, Xuqi AU - Cui X AUID- ORCID: 0009-0003-1475-5156 AD - Department of Nephrology, the First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China. FAU - Geng, Hao AU - Geng H AUID- ORCID: 0009-0007-9831-3046 AD - Department of Nephrology, the First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China. FAU - Zhang, Jian AU - Zhang J AUID- ORCID: 0009-0002-6663-608X AD - Department of Nephrology, the First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China. FAU - Bai, Xiao AU - Bai X AD - Laboratory of Epigenetics, School of Life Sciences, China Medical University, Shenyang, Liaoning, People's Republic of China. FAU - Shang, Weihao AU - Shang W AD - Laboratory of Epigenetics, School of Life Sciences, China Medical University, Shenyang, Liaoning, People's Republic of China. FAU - Sun, Li AU - Sun L AUID- ORCID: 0000-0002-9682-990X AD - Department of Nephrology, the First Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China. LA - eng PT - Comparative Study PT - Journal Article DEP - 20261024 PL - New Zealand TA - Drug Des Devel Ther JT - Drug design, development and therapy JID - 101475745 RN - WM0HAQ4WNM (Tacrolimus) RN - 0 (Immunosuppressive Agents) RN - 0 (Glucocorticoids) RN - 4F4X42SYQ6 (Rituximab) RN - 8N3DW7272P (Cyclophosphamide) SB - IM MH - Humans MH - *Tacrolimus/administration & dosage/therapeutic use/adverse effects MH - *Glomerulonephritis, Membranous/drug therapy MH - Female MH - *Immunosuppressive Agents/administration & dosage/therapeutic use/adverse effects MH - Male MH - *Glucocorticoids/administration & dosage/therapeutic use/adverse effects MH - Middle Aged MH - Dose-Response Relationship, Drug MH - Adult MH - Drug Therapy, Combination MH - Treatment Outcome MH - Rituximab/administration & dosage/therapeutic use MH - Cyclophosphamide/administration & dosage/therapeutic use MH - Retrospective Studies PMC - PMC13715842 OTO - NOTNLM OT - efficacy OT - glucocorticoid OT - high-risk group OT - membranous nephropathy OT - tacrolimus COIS- No known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/24 CRDT- 2026/10/29 06:15 PHST- 2026/07/22 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:15 [entrez] PHST- 2026/10/24 00:00 [pmc-release] AID - 639867 [pii] AID - 10.2147/DDDT.S639867 [doi] PST - epublish SO - Drug Des Devel Ther. 2026 Oct 24;20:639867. doi: 10.2147/DDDT.S639867. eCollection 2026. PMID- 42953770 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2514-2119 (Electronic) IS - 2514-2119 (Linking) VI - 10 IP - 10 DP - 2026 Oct TI - Resolution of Lancisi's sign following bicaval TricValve implantation for torrential tricuspid regurgitation: a case report defining a novel bedside marker of haemodynamic recovery. PG - ytag735 LID - 10.1093/ehjcr/ytag735 [doi] LID - ytag735 AB - BACKGROUND: Caval valve implantation (CAVI) using the TricValve system is an emerging treatment option for severe tricuspid regurgitation (TR) in patients who are unsuitable for surgery or orthotopic transcatheter intervention. Lancisi's sign is a classical manifestation of severe TR; however, its resolution after CAVI has not been described. CASE SUMMARY: An 85-year-old woman with recurrent right heart failure and torrential TR presented with prominent bilateral Lancisi's sign. Echocardiography revealed severe annular dilatation (48 mm), right ventricular dysfunction (TAPSE, 13 mm), and a left ventricular ejection fraction of 40%. Owing to prohibitive surgical risk (EuroSCORE II 18.4%; TRI SCORE 14), she underwent successful implantation of 29-mm superior vena cava and 35-mm inferior vena cava TricValve prostheses. At 3 months, Lancisi's sign had completely resolved, NYHA functional class improved from III-IV to I-II, NT-proBNP decreased from 25 000 to 2 000 pg/mL, TAPSE increased from 13 to 20 mm, and no rehospitalization or device-related adverse event occurred. We report a structured single-patient case using clinical examination, echocardiography, invasive hemodynamic assessment, laboratory markers, and 3-month follow-up after bicaval TricValve implantation. DISCUSSION: Bicaval TricValve implantation resulted in marked symptomatic, biochemical, echocardiographic, and bedside clinical improvements. The resolution of Lancisi's sign may represent a simple marker of hemodynamic recovery after CAVI. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the European Society of Cardiology. FAU - Asfour, Mohamed AU - Asfour M AUID- ORCID: 0000-0001-7181-0914 AD - Department of Cardiology, TOBB University of Economics and Technology Faculty of Medicine, Yasam Cad. No: 5, Sogutozu, Ankara 06560, Turkiye. FAU - Bozbas, Huseyin AU - Bozbas H AUID- ORCID: 0000-0002-7405-0137 AD - Department of Cardiology, TOBB University of Economics and Technology Faculty of Medicine, Yasam Cad. No: 5, Sogutozu, Ankara 06560, Turkiye. LA - eng PT - Case Reports PT - Journal Article DEP - 20261017 PL - England TA - Eur Heart J Case Rep JT - European heart journal. Case reports JID - 101730741 PMC - PMC13715628 OTO - NOTNLM OT - Case report OT - Caval valve implantation OT - Lancisi's sign OT - TricValve OT - Tricuspid regurgitation COIS- Conflict of interest. None declared EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/17 CRDT- 2026/10/29 06:15 PHST- 2026/06/12 00:00 [received] PHST- 2026/07/03 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:15 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - ytag735 [pii] AID - 10.1093/ehjcr/ytag735 [doi] PST - epublish SO - Eur Heart J Case Rep. 2026 Oct 17;10(10):ytag735. doi: 10.1093/ehjcr/ytag735. eCollection 2026 Oct. PMID- 42953760 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1178-7015 (Print) IS - 1178-7015 (Electronic) IS - 1178-7015 (Linking) VI - 19 DP - 2026 TI - Risankizumab in Psoriasis: Real-World Effectiveness, Safety, and Clinical Outcomes. PG - 624904 LID - 10.2147/CCID.S624904 [doi] LID - 624904 AB - BACKGROUND: Risankizumab (anti-IL-23 p19) is an established therapy for moderate-to-severe psoriasis, with strong trial efficacy and growing real-world evidence. However, long-duration outcomes and treatment-spacing patterns from the Gulf region remain limited. OBJECTIVE: To evaluate long-term effectiveness, safety, and treatment patterns-including extended-interval dosing-of Risankizumab in a real-world tertiary center. METHODS: Single-center retrospective cohort of adults treated with Risankizumab between January 2020 and January 2025. Primary outcome: Psoriasis Area and Severity Index (PASI) 100 on last visit. Secondary outcomes: changes in Psoriasis Area and Severity Index (PASI), body surface area (BSA), and Dermatology Life Quality Index (DLQI) and Nail Psoriasis Severity Index (NAPSI); adverse events; treatment persistence and dosing intervals. Non-parametric tests were used; predictors of PASI 100 were explored with multivariable logistic regression. RESULTS: We included 144 patients (mean age 39.3+/-14.9 years; 55.6% male; mean BMI 29.0+/-6.5). At last visit, 61.1% achieved PASI 100. Mean scores improved from baseline to last visit: PASI 7.24-->0.34, BSA 9.81%-->0.26%, DLQI 7.64-->0.68, NAPSI 9.56-->2.94 (all p<0.001). Adverse events were infrequent (3.5%), all mild; no major adverse cardiovascular events or deaths occurred. A single case of lung cancer was reported and considered unlikely to be treatment related. A quarter of patients transitioned to extended intervals (most commonly every 4-6 months), with the majority maintaining disease control and relapse occurring infrequently and only after >/=6 months. Longer treatment duration and maintenance of standard dosing frequency were associated with achieving PASI 100, while positive family history was associated with lower odds of complete clearance. CONCLUSION: In routine practice, Risankizumab achieved high complete-clearance rates with excellent tolerability over ~31 months' mean exposure. Extended-interval dosing was feasible in selected stable responders. These findings align with trial durability and regional registry signals and support individualized long-term strategies. CI - (c) 2026 Alkofide et al. FAU - Alkofide, Maha AU - Alkofide M AUID- ORCID: 0009-0000-6510-9427 AD - Department of Dermatology, College of Medicine, King Saud University, Riyadh, Saudi Arabia. FAU - Madani, Abdulaziz AU - Madani A AD - Department of Dermatology, College of Medicine, King Saud University, Riyadh, Saudi Arabia. FAU - Alsukait, Sarah AU - Alsukait S AD - Department of Dermatology, College of Medicine, King Saud University, Riyadh, Saudi Arabia. FAU - Alotaibi, Hend AU - Alotaibi H AUID- ORCID: 0000-0003-4290-8327 AD - Department of Dermatology, College of Medicine, King Saud University, Riyadh, Saudi Arabia. FAU - Almaqbali, Huda Salim AU - Almaqbali HS AD - Department of Dermatology, College of Medicine, King Saud University, Riyadh, Saudi Arabia. FAU - Alshehri, Naif Ahmed AU - Alshehri NA AUID- ORCID: 0009-0004-4248-8312 AD - Department of Dermatology, College of Medicine, King Saud University, Riyadh, Saudi Arabia. FAU - Barakeh, Maee AU - Barakeh M AD - Department of Dermatology, King Abdullah Bin Abdulaziz University Hospital, Riyadh, Saudi Arabia. FAU - Alharbi, Bandar Ahmed AU - Alharbi BA AD - Department of Dermatology, Riyadh First Health Cluster, King Saud Medical City, Riyadh, Saudi Arabia. FAU - Nagshabandi, Khalid Nabil AU - Nagshabandi KN AUID- ORCID: 0009-0008-6577-6463 AD - Department of Dermatology, College of Medicine, King Saud University, Riyadh, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261024 PL - New Zealand TA - Clin Cosmet Investig Dermatol JT - Clinical, cosmetic and investigational dermatology JID - 101543449 PMC - PMC13715541 OTO - NOTNLM OT - PASI OT - dosing interval OT - psoriasis OT - real-world evidence OT - risankizumab OT - safety COIS- The authors declare no conflicts of interest in this work. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/24 CRDT- 2026/10/29 06:15 PHST- 2026/05/15 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:15 [entrez] PHST- 2026/10/24 00:00 [pmc-release] AID - 624904 [pii] AID - 10.2147/CCID.S624904 [doi] PST - epublish SO - Clin Cosmet Investig Dermatol. 2026 Oct 24;19:624904. doi: 10.2147/CCID.S624904. eCollection 2026. PMID- 42953759 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1179-1357 (Print) IS - 1179-1357 (Electronic) IS - 1179-1357 (Linking) VI - 18 DP - 2026 TI - A Stage-Based Decision Framework to Minimize Excess Cement in Cement-Retained Implant Restorations: A Narrative Review. PG - 628399 LID - 10.2147/CCIDE.S628399 [doi] LID - 628399 AB - BACKGROUND: Residual excess cement (REC) has been identified as an iatrogenic factor associated with peri-implant mucositis and peri-implantitis. The detection and removal of subgingival cement are considered clinically challenging, particularly in cases with deep margins. OBJECTIVE: This narrative review aims to identify available clinical techniques used to minimize residual excess cement (REC) in cement-retained implant restorations, categorize these techniques according to pre-cementation, cementation, and post-cementation phases, and relate technique selection to clinical risk according to margin depth and clinical accessibility. Low risk (equigingival or 2 mm subgingival or poor access). METHODS: An electronic search of PubMed/MEDLINE, Scopus, and Web of Science was conducted for English-language publications available through 28 February 2026 using seven complementary Boolean search combinations addressing residual/excess cement, peri-implant disease, cementation techniques, margin/abutment design, detection/removal, venting, and barrier techniques. The initial searches identified 929 records. After removal of 435 duplicates, the remaining 494 articles were screened for title and abstract,380 were excluded. 114 full text articles were assessed. Of these, 76 were excluded after full-text assessment and 38 publications were included in this narrative review. Due to heterogeneity in study designs and outcomes, findings were synthesized narratively rather than quantitatively. RESULTS: Strategies were categorized into pre-cementation, cementation, and post-cementation. Low risk cases were managed with controlled cement volume, accessible margin designs and post cementation verification. Moderate- and high-risk cases favor custom margin design, radiopaque cement, extraoral cement-volume control, and selected barrier and/or venting measures. In high-risk cases,combining multiple techniques may be presented as an evidence informed option rather than validated protocols. CONCLUSION: Stage-based risk management offers clinicians an evidence-informed approach to technique selection. Margin depth and accessibility may be considered as a guide for selection of cementation strategies. In high-risk situations, combining multiple techniques may be reasonable, but specific combinations have not been prospectively validated and should not be regarded as definitive clinical protocols. CI - (c) 2026 Al-Jallad. FAU - Al-Jallad, Walid AU - Al-Jallad W AUID- ORCID: 0000-0003-4644-9316 AD - Department of Prosthodontics, Faculty of Dentistry, Arab American University, Jenin, Palestine. LA - eng PT - Journal Article PT - Review DEP - 20261024 PL - New Zealand TA - Clin Cosmet Investig Dent JT - Clinical, cosmetic and investigational dentistry JID - 101579785 PMC - PMC13715526 OTO - NOTNLM OT - CAD/CAM OT - cementation OT - dental OT - dental cements OT - dental implants OT - dental prosthesis OT - implant-supported OT - peri-implantitis OT - prosthesis retention OT - radiography COIS- The author declares no competing interests. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/24 CRDT- 2026/10/29 06:15 PHST- 2026/05/28 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:15 [entrez] PHST- 2026/10/24 00:00 [pmc-release] AID - 628399 [pii] AID - 10.2147/CCIDE.S628399 [doi] PST - epublish SO - Clin Cosmet Investig Dent. 2026 Oct 24;18:628399. doi: 10.2147/CCIDE.S628399. eCollection 2026. PMID- 42953748 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2514-2119 (Electronic) IS - 2514-2119 (Linking) VI - 10 IP - 10 DP - 2026 Oct TI - Generalized myoclonus following dobutamine stress echocardiography: a case report. PG - ytag775 LID - 10.1093/ehjcr/ytag775 [doi] LID - ytag775 AB - BACKGROUND: Dobutamine stress echocardiography (DSE) is a widely used and generally safe non-invasive imaging modality for the assessment of myocardial ischaemia. Adverse effects are predominantly cardiovascular, whereas neurological complications are exceedingly rare. Dobutamine-induced myoclonus (DIM) has only rarely been reported, mainly in patients receiving prolonged dobutamine infusions for heart failure or cardiomyopathy. CASE SUMMARY: A 47-year-old woman underwent DSE for evaluation of exertional chest pain. Following completion of a standard dobutamine protocol, she developed generalized rhythmic myoclonic jerks predominantly involving the axial musculature. The patient remained fully conscious and hemodynamically stable throughout the episode. Intravenous diazepam failed to improve symptoms, whereas complete resolution occurred after administration of intravenous valproic acid. Laboratory investigations, including electrolytes, renal function, and thyroid function tests, were normal. Electroencephalography and brain magnetic resonance imaging revealed no abnormalities. No recurrence was observed during hospitalization or follow-up. DISCUSSION: To our knowledge, this is the first reported case of DIM occurring immediately after DSE. Unlike previously published cases, which predominantly involved patients with chronic kidney disease and developed after prolonged dobutamine exposure, our patient had normal renal function and developed symptoms immediately upon completion of the stress protocol. The pathophysiology remains uncertain, although increased central nervous system exposure to dobutamine has been proposed. Clinicians performing DSE should be aware of myoclonus as a rare but important neurological complication and should consider valproic acid when benzodiazepines fail to achieve symptom control. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the European Society of Cardiology. FAU - Bontozis, Anastasios AU - Bontozis A AUID- ORCID: 0009-0009-4704-2973 AD - Department of Anesthesiology, Asklepieion General Hospital of Voula, Vas. Pavlou Ave. 1, Athens 16673, Greece. FAU - Makris, Alexandros AU - Makris A AUID- ORCID: 0000-0001-5299-1553 AD - Department of Anesthesiology, Asklepieion General Hospital of Voula, Vas. Pavlou Ave. 1, Athens 16673, Greece. FAU - Iliopoulos, Dimitrios AU - Iliopoulos D AUID- ORCID: 0009-0009-5138-5434 AD - Department of Cardiology, Asklepieion General Hospital of Voula, Vas. Pavlou Ave. 1, Athens 16673, Greece. FAU - Kotoulas, Sotirios AU - Kotoulas S AUID- ORCID: 0009-0000-1785-3198 AD - Department of Cardiology, Asklepieion General Hospital of Voula, Vas. Pavlou Ave. 1, Athens 16673, Greece. FAU - Chamodraka, Eftyhia AU - Chamodraka E AD - Department of Cardiology, Asklepieion General Hospital of Voula, Vas. Pavlou Ave. 1, Athens 16673, Greece. LA - eng PT - Case Reports PT - Journal Article DEP - 20261021 PL - England TA - Eur Heart J Case Rep JT - European heart journal. Case reports JID - 101730741 PMC - PMC13715625 OTO - NOTNLM OT - Adverse drug reaction OT - Case report OT - Dobutamine OT - Myoclonus OT - Neurological complication OT - Stress echocardiography COIS- Conflict of interest. None declared. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/21 CRDT- 2026/10/29 06:14 PHST- 2026/08/22 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:14 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ytag775 [pii] AID - 10.1093/ehjcr/ytag775 [doi] PST - epublish SO - Eur Heart J Case Rep. 2026 Oct 21;10(10):ytag775. doi: 10.1093/ehjcr/ytag775. eCollection 2026 Oct. PMID- 42953746 OWN - NLM STAT- Publisher LR - 20261029 IS - 2046-2069 (Electronic) IS - 2046-2069 (Linking) DP - 2026 Oct 28 TI - Kaolin-derived Cu(ii)-doped zeolite supported on nickel foam for electrochemical paracetamol detection. LID - 10.1039/d6ra09658a [doi] AB - A kaolin-derived Cu(ii)-doped zeolite (Cu-Z(1)) was synthesized through metakaolin activation, alkaline crystallization, and subsequent Cu(2+) ion exchange, and immobilized on nickel foam (Cu-Z(1)/NiF) for electrochemical determination of paracetamol. The synthesized materials were characterized using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy coupled with energy-dispersive X-ray spectroscopy (SEM/EDS), X-ray photoelectron spectroscopy (XPS), and thermogravimetric/differential thermogravimetric analysis (TGA/DTGA). The characterization results confirmed preservation of a ZSM-5-type aluminosilicate framework following Cu(ii) incorporation, with accompanying changes in crystallinity, morphology, and surface chemical environment. Electrochemical measurements demonstrated enhanced paracetamol oxidation at Cu-Z(1)/NiF compared with bare nickel foam and undoped Z(1)/NiF, giving an anodic peak current of 75.69 microA, a midpoint potential of 355 mV, and reduced charge-transfer resistance. Under optimized conditions, differential pulse voltammetry (DPV) showed a linear response over 5-100 microM paracetamol, with a detection limit of 0.3926 microM, sensitivity of 2.0456 microA microM(-1) cm(-2), and R (2) = 0.9971. The sensor exhibited good selectivity toward paracetamol in the presence of dopamine, uric acid, and glucose, with repeatability below 0.8% RSD. It retained 95.8% of its initial response after 20 days. Analysis of commercial paracetamol tablets produced recoveries of 95.91-99.29%, demonstrating the applicability of the Cu-Z(1)/NiF sensor for pharmaceutical paracetamol determination. CI - This journal is (c) The Royal Society of Chemistry. FAU - Chemere, Endazenaw Bizuneh AU - Chemere EB AUID- ORCID: 0000-0003-1984-912X AD - Institute for Catalysis and Energy Solutions, College of Science, Engineering and Technology (CSET), University of South Africa (UNISA) Florida 1709 Johannesburg South Africa chemeeb@unisa.ac.za maboywk@unisa.ac.za makgwpr@unisa.ac.za adekuas@unisa.ac.za. FAU - Jigso, Chernet Beraso AU - Jigso CB AD - Department of Chemistry, College of Natural and Computational Sciences, Dilla University Dilla Ethiopia cberaso22@gmail.com. FAU - Mhike, Washington AU - Mhike W AUID- ORCID: 0000-0003-4670-1171 AD - Department of Chemical, Metallurgical and Materials Engineering, Tshwane University of Technology Pretoria South Africa MhikeW@tut.ac.za MavhunguL@tut.ac.za. FAU - Mavhungu, Mapula Lucey AU - Mavhungu ML AD - Department of Chemical, Metallurgical and Materials Engineering, Tshwane University of Technology Pretoria South Africa MhikeW@tut.ac.za MavhunguL@tut.ac.za. FAU - Maboya, Winny Kgabo AU - Maboya WK AD - Institute for Catalysis and Energy Solutions, College of Science, Engineering and Technology (CSET), University of South Africa (UNISA) Florida 1709 Johannesburg South Africa chemeeb@unisa.ac.za maboywk@unisa.ac.za makgwpr@unisa.ac.za adekuas@unisa.ac.za. FAU - Ramashadi, Makgwane Peter AU - Ramashadi MP AD - Institute for Catalysis and Energy Solutions, College of Science, Engineering and Technology (CSET), University of South Africa (UNISA) Florida 1709 Johannesburg South Africa chemeeb@unisa.ac.za maboywk@unisa.ac.za makgwpr@unisa.ac.za adekuas@unisa.ac.za. FAU - Adekunle, Abolanle Saheed AU - Adekunle AS AD - Institute for Catalysis and Energy Solutions, College of Science, Engineering and Technology (CSET), University of South Africa (UNISA) Florida 1709 Johannesburg South Africa chemeeb@unisa.ac.za maboywk@unisa.ac.za makgwpr@unisa.ac.za adekuas@unisa.ac.za. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - RSC Adv JT - RSC advances JID - 101581657 PMC - PMC13715543 COIS- The authors declare no competing financial interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 PMCR- 2026/10/28 CRDT- 2026/10/29 06:14 PHST- 2026/10/03 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:14 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - d6ra09658a [pii] AID - 10.1039/d6ra09658a [doi] PST - aheadofprint SO - RSC Adv. 2026 Oct 28. doi: 10.1039/d6ra09658a. PMID- 42953742 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1178-2390 (Print) IS - 1178-2390 (Electronic) IS - 1178-2390 (Linking) VI - 19 DP - 2026 TI - Best Evidence Summary for Peri-Extubation Management Among Difficult-to-Wean Patients in the Intensive Care Unit. PG - 641498 LID - 10.2147/JMDH.S641498 [doi] LID - 641498 AB - OBJECTIVE: To systematically synthesize the best available domestic and international evidence regarding peri-extubation management among difficult-to-wean ICU patients, so as to offer evidence-based recommendations for routine clinical practice. METHODS: A systematic search was conducted across various domestic and international databases and websites, including Up To Date, National Institute of Health and Clinical Excellence (NICE), National Guideline Clearinghouse (NGC), Guideline International Network (GIN), Chinese Medical Journal Full-text Database, China National Knowledge Infrastructure (CNKI), Yimaitong, Wanfang Database, and VIP Database, to obtain the best evidence on peri-extubation management for ICU patients with difficult extubation, including guidelines, expert consensus documents, clinical decision-making materials, systematic reviews or meta-analyses, and randomized controlled trials (RCTs). The search period covered from the establishment of each database until March 2026. Literature quality assessment was independently performed by two researchers who have published evidence-based research articles and received professional evidence-based training, with expert input solicited; eligible literature was selected for evidence extraction and summarization. RESULTS: This study ultimately included 23 publications, comprising 8 guidelines, 5 expert consensus documents, 8 systematic reviews, and 2 randomized controlled trials (RCTs). Through analysis, organization, and evidence synthesis of the included literature, evidence was summarized across seven key areas: multidisciplinary team (MDT) collaboration, pre-weaning preparation and assessment, comprehensive management strategies for difficult weaning, management of special patient populations, intubation removal strategies during weaning, post-intubation management strategies, and health education, encompassing 48 pieces of best available evidence. CONCLUSION: This study summarizes the optimal evidence for peri-extubation management in patients with difficult weaning from seven perspectives, providing guidance for ICU clinicians to develop and implement tailored weaning protocols aimed at improving clinical outcomes and quality of life for these patients. CI - (c) 2026 Sun et al. FAU - Sun, Ruixiang AU - Sun R AD - Department of Critical Care Medicine, The First Affiliated Hospital of Wannan Medical University (Yijishan Hospital), Wuhu, Anhui Province, 241000, People's Republic of China. FAU - Tang, Huixian AU - Tang H AD - Graduate School of Wannan Medical University, Wuhu, Anhui Province, 241000, People's Republic of China. FAU - Fang, Ke AU - Fang K AD - Department of Critical Care Medicine, The First Affiliated Hospital of Wannan Medical University (Yijishan Hospital), Wuhu, Anhui Province, 241000, People's Republic of China. FAU - Wu, Mingxing AU - Wu M AD - Department of Critical Care Medicine, The First Affiliated Hospital of Wannan Medical University (Yijishan Hospital), Wuhu, Anhui Province, 241000, People's Republic of China. FAU - Xu, Ping AU - Xu P AD - Department of Critical Care Medicine, The First Affiliated Hospital of Wannan Medical University (Yijishan Hospital), Wuhu, Anhui Province, 241000, People's Republic of China. FAU - Zhang, Feng AU - Zhang F AD - Neurosurgery Intensive Care Unit, The First Affiliated Hospital of Wannan Medical University (Yijishan Hospital), Wuhu, Anhui Province, 241000, People's Republic of China. FAU - Gao, Hong AU - Gao H AD - Department of Critical Care Medicine, The First Affiliated Hospital of Wannan Medical University (Yijishan Hospital), Wuhu, Anhui Province, 241000, People's Republic of China. LA - eng PT - Journal Article PT - Review DEP - 20261024 PL - New Zealand TA - J Multidiscip Healthc JT - Journal of multidisciplinary healthcare JID - 101512691 PMC - PMC13715538 OTO - NOTNLM OT - ICU OT - best available evidence OT - difficult extubation OT - management during the peri-extubation period COIS- None of the authors have any financial disclosure or conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/24 CRDT- 2026/10/29 06:14 PHST- 2026/07/17 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:14 [entrez] PHST- 2026/10/24 00:00 [pmc-release] AID - 641498 [pii] AID - 10.2147/JMDH.S641498 [doi] PST - epublish SO - J Multidiscip Healthc. 2026 Oct 24;19:641498. doi: 10.2147/JMDH.S641498. eCollection 2026. PMID- 42953734 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2514-2119 (Electronic) IS - 2514-2119 (Linking) VI - 10 IP - 10 DP - 2026 Oct TI - Novel cardiovascular magnetic resonance imaging biomarkers to track response to treatment including Sotatercept in a patient with idiopathic pulmonary arterial hypertension: a case report. PG - ytag763 LID - 10.1093/ehjcr/ytag763 [doi] LID - ytag763 AB - BACKGROUND: Pulmonary arterial hypertension (PAH) causes progressive right ventricular (RV) pressure overload and left ventricular (LV) underfilling. Sotatercept is an activin-signal inhibitor, approved by FDA in 2024 and EMA in 2025, as an add-on treatment in adult patients with PAH. Cardiovascular magnetic resonance (CMR)-derived atrioventricular plane displacement (AVPD) and left ventricular pressure-volume (PV) loops may represent non-invasive markers of treatment response, particularly important as novel therapies addressing remodelling emerge. CASE SUMMARY: A 30-year-old woman was diagnosed with idiopathic PAH, after presenting with dyspnoea and peripheral oedema. Baseline CMR showed severe RV dilation, reduced RV ejection fraction, and low indexed LV stroke volume, with reduced bilateral AVPD and markedly impaired LV PV loop indices, with low stroke work, low mean external power, and high ventriculo-arterial coupling. Initial dual PAH therapy, with an endothelin receptor antagonist and a phosphodiesterase-5 inhibitor initially improved symptoms, pulmonary vascular resistance, as well as AVPD and PV loop derived indices, whereas conventional CMR indices showed only minimal change. One year later, despite triple therapy, including prostacyclin analogue, the patient deteriorated clinically and CMR showed worsening of AVPD and PV loop indices. Treatment escalation with Sotatercept immediately improved clinical status, haemodynamic, AVPD, and PV loop indices despite persistent RV remodelling. DISCUSSION: Serial CMR-derived AVPD and LV PV loops may provide clinically useful additional information during longitudinal follow-up of PAH patients, especially in those approaching maximal available medical therapies. In the present case, both AVPD and LV PV loops reflected treatment response, and showed significant improvement after initiation of Sotatercept. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the European Society of Cardiology. FAU - Castiglione, Alessandro AU - Castiglione A AUID- ORCID: 0000-0002-5380-8789 AD - Clinical Physiology, Department of Clinical Sciences Lund, Lund University, Entregatan 7, 222 42 Lund, Sweden. AD - Department of Clinical Physiology, Skane University Hospital, Entregatan 7, 222 42 Lund, Sweden. FAU - Kjellstrom, Barbro AU - Kjellstrom B AUID- ORCID: 0000-0002-7936-1209 AD - Clinical Physiology, Department of Clinical Sciences Lund, Lund University, Entregatan 7, 222 42 Lund, Sweden. AD - Department of Clinical Physiology, Skane University Hospital, Entregatan 7, 222 42 Lund, Sweden. FAU - Radegran, Goran AU - Radegran G AUID- ORCID: 0000-0003-4919-2771 AD - Cardiology, Department of Clinical Sciences Lund, Lund University, Skanes universitetssjukhus, EA-blocket, 221 85 Lund, Sweden. AD - Haemodynamic Lab, Section for Heart Failure and Valvular Disease, Skane University Hospital, Entregatan 7, 222 43 Lund, Sweden. FAU - Ostenfeld, Ellen AU - Ostenfeld E AUID- ORCID: 0000-0002-2655-2423 AD - Clinical Physiology, Department of Clinical Sciences Lund, Lund University, Entregatan 7, 222 42 Lund, Sweden. AD - Department of Clinical Physiology, Skane University Hospital, Entregatan 7, 222 42 Lund, Sweden. LA - eng PT - Case Reports PT - Journal Article DEP - 20261026 PL - England TA - Eur Heart J Case Rep JT - European heart journal. Case reports JID - 101730741 PMC - PMC13715627 OTO - NOTNLM OT - Atrioventricular plane displacement OT - Cardiac imaging biomarkers OT - Case report OT - Pressure-volume loops OT - Pulmonary arterial hypertension OT - Sotatercept OT - Treatment monitoring COIS- Conflict of interest. The authors have no competing financial interests or personal relationships that could influence the work reported. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/26 CRDT- 2026/10/29 06:13 PHST- 2026/07/03 00:00 [received] PHST- 2026/08/14 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:13 [entrez] PHST- 2026/10/26 00:00 [pmc-release] AID - ytag763 [pii] AID - 10.1093/ehjcr/ytag763 [doi] PST - epublish SO - Eur Heart J Case Rep. 2026 Oct 26;10(10):ytag763. doi: 10.1093/ehjcr/ytag763. eCollection 2026 Oct. PMID- 42953730 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2053-714X (Electronic) IS - 2095-5138 (Print) IS - 2053-714X (Linking) VI - 13 IP - 19 DP - 2026 Oct TI - Moderate prevalence and evolutionary causes of paralogous compensation in humans. PG - nwag565 LID - 10.1093/nsr/nwag565 [doi] LID - nwag565 AB - Paralogous compensation drives genetic robustness; however, its in vivo prevalence in humans and the evolutionary forces shaping it remain unresolved. We CRISPR-screened 678 human paralog pairs to identify pairs with negative genetic interactions (nGIs) or compensatory paralogs, and integrated our results with four studies to assemble two complementary resources: the 2168-pair Union and 4859-pair Ito datasets. We extrapolated to the organismal level by classifying paralogs as core (broadly compensating, 99 Union/120 Ito pairs), conditional (111/566) or non-interacting (1958/4173), and testing for enriched compensatory signals in core pairs relative to the latter two groups. Core nGI pairs consistently showed prenatal co-expression, depletion of disease genes and enrichment for upregulation after paralog loss in cancer, though the signals were often moderate. Evolutionary analyses found similar fractions ( approximately 2%) of core compensators among both ancient and young paralogs. Ancient pairs retain overlaps likely under pleiotropic and dosage constraints. Incomplete small-scale duplication mechanisms limit compensation among young pairs, except X-derived retrogenes, which are overrepresented among core nGIs ( approximately 14%). This is consistent with selection to counteract male germline X inactivation and subsequent out-of-testis expansion. Overall, paralogous compensation is moderately prevalent in vivo in humans, shaped by multiple evolutionary forces acting on ancient or young duplicates. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of China Science Publishing & Media Ltd. FAU - Shen, Jieyu AU - Shen J AD - State Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. AD - University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Yu, Daqi AU - Yu D AD - State Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. AD - University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Wang, Wenjia AU - Wang W AD - State Key Laboratory of Common Mechanism Research for Major Diseases, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China. FAU - Su, Tianhan AU - Su T AD - State Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. AD - University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Wang, Dingding AU - Wang D AD - State Key Laboratory of Common Mechanism Research for Major Diseases, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China. FAU - Liang, Junbo AU - Liang J AD - State Key Laboratory of Common Mechanism Research for Major Diseases, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences & School of Basic Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China. FAU - Guo, Yiqiong AU - Guo Y AD - State Key Laboratory of Complex, Severe, and Rare Diseases, Center for Bioinformatics, National Infrastructures for Translational Medicine, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China. FAU - Zhao, Shangqi AU - Zhao S AD - State Key Laboratory of Complex, Severe, and Rare Diseases, Center for Bioinformatics, National Infrastructures for Translational Medicine, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China. FAU - Ma, Huijing AU - Ma H AD - State Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. FAU - Wang, Jichang AU - Wang J AD - Key Laboratory for Stem Cells and Tissue Engineering (Sun Yat-sen University), Ministry of Education, Guangzhou 510080, China. AD - Department of Histology and Embryology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, China. FAU - Wang, Xiaoyue AU - Wang X AUID- ORCID: 0000-0002-0208-5322 AD - State Key Laboratory of Complex, Severe, and Rare Diseases, Center for Bioinformatics, National Infrastructures for Translational Medicine, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China. FAU - Zhang, Yong E AU - Zhang YE AD - State Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China. AD - University of Chinese Academy of Sciences, Beijing 100049, China. LA - eng PT - Journal Article DEP - 20260903 PL - China TA - Natl Sci Rev JT - National science review JID - 101633095 PMC - PMC13715443 OTO - NOTNLM OT - CRISPR screening OT - gene duplication OT - genetic robustness OT - paralogous compensation OT - pleiotropy OT - retrogene EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/03 CRDT- 2026/10/29 06:13 PHST- 2026/01/09 00:00 [received] PHST- 2026/07/22 00:00 [revised] PHST- 2026/08/04 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:13 [entrez] PHST- 2026/09/03 00:00 [pmc-release] AID - nwag565 [pii] AID - 10.1093/nsr/nwag565 [doi] PST - epublish SO - Natl Sci Rev. 2026 Sep 3;13(19):nwag565. doi: 10.1093/nsr/nwag565. eCollection 2026 Oct. PMID- 42953709 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2771-9316 (Electronic) IS - 2771-9316 (Linking) VI - 4 IP - 10 DP - 2026 Oct 26 TI - Electrochemistry of Urea Synthesis via Carbon Dioxide Reduction: Mechanisms and Material Insights. PG - 1533-1565 LID - 10.1021/prechem.6c00062 [doi] AB - Electrocatalytic urea synthesis offers a sustainable, carbon- and energy-efficient alternative to the Haber-Bosch process under ambient conditions. It is a sustainable strategy in which simultaneous reduction of carbon dioxide (CO(2)) and nitrogen molecules (nitrogen, nitrate, nitrite, nitric oxide, etc.) lead to form urea. However, electrochemical urea synthesis faces multiple challenges including activation of CO(2) and nitrogen molecules (nitrogen, nitrate, nitrite, nitric oxide, etc.), low production and selectivity toward urea production. The C-N coupling reaction is considered the most challenging part for urea synthesis. With respect to all this, this review focuses on fundamental reaction mechanisms followed by recent progress for electrochemical urea production. Recent advances highlight the rational design of heterogeneous catalystsranging from transition-metal-based systems (Cu, Ni, Fe, Co) to single-atom catalyststhat enable simultaneous activation of CO(2) and nitrogen molecules. This review summarizes proposed reaction pathways by correlating catalyst active centers with mechanistic insights into CO(2)/N-containing species activation and coupling. The critical challenges related to low yield and Faradaic efficiency are outlined, providing perspectives for translating laboratory breakthroughs into practical urea electrosynthesis technologies. Collectively, these advances establish electrocatalytic urea synthesis as a frontier in sustainable chemistry, bridging carbon capture with nitrogen cycle management, and paving the way toward scalable green urea production. CI - (c) 2026 The Authors. Co-published by University of Science and Technology of China and American Chemical Society. FAU - Ingavale, Sagar AU - Ingavale S AD - School of Bio-Chemical Engineering and Technology, Sirindhorn International Institute of Technology, Thammasat University, Khlong Luang, Pathum Thani 12120, Thailand. RINGGOLD: 37698 AD - Research Unit in Sustainable Electrochemical Intelligent, Thammasat University, Khlong Luang, Pathum Thani 12120, Thailand. RINGGOLD: 37698 FAU - Marbaniang, Phiralang AU - Marbaniang P AD - Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand. RINGGOLD: 26683 FAU - Bunyanidhi, Panyawee AU - Bunyanidhi P AD - School of Bio-Chemical Engineering and Technology, Sirindhorn International Institute of Technology, Thammasat University, Khlong Luang, Pathum Thani 12120, Thailand. RINGGOLD: 37698 AD - Research Unit in Sustainable Electrochemical Intelligent, Thammasat University, Khlong Luang, Pathum Thani 12120, Thailand. RINGGOLD: 37698 FAU - Pornrungroj, Chanon AU - Pornrungroj C AUID- ORCID: 0000-0001-9886-8489 AD - Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand. RINGGOLD: 26683 AD - Bio-Circular-Green-economy Technology & Engineering Center (BCGeTEC), Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand. RINGGOLD: 26683 AD - Photocatalysts for Clean Environment and Energy Research Unit, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok 10330, Thailand. RINGGOLD: 26683 FAU - Iamprasertkun, Pawin AU - Iamprasertkun P AUID- ORCID: 0000-0001-8950-3330 AD - School of Bio-Chemical Engineering and Technology, Sirindhorn International Institute of Technology, Thammasat University, Khlong Luang, Pathum Thani 12120, Thailand. RINGGOLD: 37698 AD - Research Unit in Sustainable Electrochemical Intelligent, Thammasat University, Khlong Luang, Pathum Thani 12120, Thailand. RINGGOLD: 37698 LA - eng PT - Journal Article PT - Review DEP - 20260817 PL - United States TA - Precis Chem JT - Precision chemistry JID - 9918574281706676 PMC - PMC13715327 OTO - NOTNLM OT - Bimetallic catalysts OT - CO2 reduction reaction OT - C-N coupling OT - Defect engineering OT - Electrochemical OT - Nitrate reduction reaction OT - Single-atom catalysts OT - Urea synthesis EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/08/17 CRDT- 2026/10/29 06:12 PHST- 2026/05/08 00:00 [received] PHST- 2026/07/26 00:00 [revised] PHST- 2026/07/29 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:12 [entrez] PHST- 2026/08/17 00:00 [pmc-release] AID - 10.1021/prechem.6c00062 [doi] PST - epublish SO - Precis Chem. 2026 Aug 17;4(10):1533-1565. doi: 10.1021/prechem.6c00062. eCollection 2026 Oct 26. PMID- 42953694 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2666-6383 (Electronic) IS - 2666-6383 (Linking) VI - 10 IP - 6 DP - 2026 Nov TI - Is the annular ligament disrupted in Monteggia fracture-dislocations? A cadaveric mechanistic study. PG - 101798 LID - 10.1016/j.jseint.2026.101798 [doi] LID - 101798 AB - BACKGROUND: Monteggia fracture-dislocation is characterized by an ulnar fracture with radial head dislocation. The mechanisms of dislocation and annular ligament integrity remain unclear. This study established a cadaveric Monteggia fracture-dislocation model to investigate radial head dislocation and associated annular ligament injury. METHODS: Twelve upper limbs from 6 fresh-frozen cadavers (mean age at death, 83 years; 1 male, 5 females) were examined. Soft tissues were removed while preserving the bones, ligaments, capsules, forearm interosseous membrane, and biceps tendon insertions. The humeroulnar joint was fixed at 90 degrees flexion, and the ulna was manually fractured to create a Bado type I (direct-blow) Monteggia fracture-dislocation model. The annular ligament, quadrate ligament, oblique cord (Weitbrecht ligament), and forearm interosseous membrane were evaluated. RESULTS: Ulnar fracture created forearm deformity, causing the radius to contact the triangular fulcrum. Continued manual loading caused the radius to pivot around the fulcrum. The annular ligament remained intact in 11 of 12 limbs; 1 specimen showed injury at the annular ligament-capsular junction. In 11 limbs, the radial head was dislocated beneath the distal margin of the annular ligament. One quadrate ligament rupture occurred; the oblique cord and forearm interosseous membrane remained intact. CONCLUSION: In this standardized cadaveric direct-blow model, anterior radial head dislocation occurred in 11 of 12 limbs, beneath the distal margin of an intact annular ligament. These findings demonstrate that annular ligament rupture is not required for radial head dislocation in the described experimental setting. This mechanism provides a possible anatomic explanation for difficult reduction in selected cases. CI - (c) 2026 The Authors. FAU - Tada, Takuya AU - Tada T AD - Department of Orthopedic Surgery, Kitasato University School of Medicine, Sagamihara City, Kanagawa, Japan. AD - Kitasato University Graduate School of Medical Sciences, Sagamihara City, Kanagawa, Japan. FAU - Sukegawa, Koji AU - Sukegawa K AD - Department of Orthopedic Surgery, Kitasato University School of Medicine, Sagamihara City, Kanagawa, Japan. AD - Department of Clinical Anatomy, Research and Development Center for Medical Education, Kitasato University School of Medicine, Sagamihara City, Kanagawa, Japan. FAU - Metoki, Yukie AU - Metoki Y AD - Department of Orthopedic Surgery, Kitasato University School of Medicine, Sagamihara City, Kanagawa, Japan. AD - Kitasato University Graduate School of Medical Sciences, Sagamihara City, Kanagawa, Japan. FAU - Hirukawa, Kohei AU - Hirukawa K AD - Department of Orthopedic Surgery, Kitasato University School of Medicine, Sagamihara City, Kanagawa, Japan. AD - Kitasato University Graduate School of Medical Sciences, Sagamihara City, Kanagawa, Japan. FAU - Mizuhashi, Tomomi AU - Mizuhashi T AD - Kitasato University Graduate School of Medical Sciences, Sagamihara City, Kanagawa, Japan. FAU - Onuma, Kenji AU - Onuma K AD - Department of Orthopedic Surgery, Kitasato University School of Medicine, Sagamihara City, Kanagawa, Japan. FAU - Otake, Yuya AU - Otake Y AD - Department of Orthopedic Surgery, Kitasato University School of Medicine, Sagamihara City, Kanagawa, Japan. FAU - Shin, Hunil AU - Shin H AD - Department of Orthopedic Surgery, Kitasato University School of Medicine, Sagamihara City, Kanagawa, Japan. FAU - Uchida, Kentaro AU - Uchida K AD - Department of Orthopedic Surgery, Kitasato University School of Medicine, Sagamihara City, Kanagawa, Japan. FAU - Ogawa, Motoyuki AU - Ogawa M AD - Department of Clinical Anatomy, Research and Development Center for Medical Education, Kitasato University School of Medicine, Sagamihara City, Kanagawa, Japan. AD - Department of Anatomy, School of Medicine, Kitasato University School of Medicine, Sagamihara City, Kanagawa, Japan. FAU - Inoue, Gen AU - Inoue G AD - Department of Orthopedic Surgery, Kitasato University School of Medicine, Sagamihara City, Kanagawa, Japan. FAU - Takaso, Masashi AU - Takaso M AD - Department of Orthopedic Surgery, Kitasato University School of Medicine, Sagamihara City, Kanagawa, Japan. LA - eng PT - Journal Article DEP - 20260911 PL - United States TA - JSES Int JT - JSES international JID - 101763461 PMC - PMC13715306 OTO - NOTNLM OT - Annular ligament OT - Bado classification OT - Interosseous ligament OT - Monteggia fracture-dislocation OT - Quadrate ligament OT - Radial head dislocation OT - Ulnar fracture EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/11 CRDT- 2026/10/29 06:12 PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:12 [entrez] PHST- 2026/09/11 00:00 [pmc-release] AID - S2666-6383(26)00183-0 [pii] AID - 101798 [pii] AID - 10.1016/j.jseint.2026.101798 [doi] PST - epublish SO - JSES Int. 2026 Sep 11;10(6):101798. doi: 10.1016/j.jseint.2026.101798. eCollection 2026 Nov. PMID- 42953689 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2771-9316 (Electronic) IS - 2771-9316 (Linking) VI - 4 IP - 10 DP - 2026 Oct 26 TI - Precise Integration of Photosensitizing and Catalytic Units into Stable Metal-Organic Frameworks for Efficient CO(2) Reduction. PG - 1639-1644 LID - 10.1021/prechem.6c00014 [doi] AB - A series of multifunctional metal-organic framework (MOF) photocatalysts, Hf-IrF-DBP-M (M = Fe, Co, or Ni), were developed using a modular postsynthetic strategy that precisely integrates Ir-based photosensitizers (Ir-PSs) with transition metal catalytic units into a Hf-based MOF. This design enables systematic tuning of the interaction between metal sites and Ir-PSs to enhance photocatalytic CO(2) reduction. Among these, Hf-IrF-DBP-Fe exhibited the best performance, achieving CO turnover numbers of 3361 after 5 h and 8510 after 24 h of visible-light irradiation, with CO selectivity exceeding 98%. This activity is approximately 187 times higher than that of the Ir-PS-only control. Fluorescence quenching and electrochemical studies revealed efficient electron transfer from photoexcited and reduced Ir-PSs to the Fe center, facilitated by the approximately 6 A spatial proximity that promotes charge transport. Moreover, the Fe-based MOF also demonstrated excellent structural stability and recyclability, underscoring its potential as a robust platform for solar-driven CO(2) conversion. CI - (c) 2026 The Authors. Co-published by University of Science and Technology of China and American Chemical Society. FAU - Liao, Yingling AU - Liao Y AD - Department of Chemistry, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, United States. RINGGOLD: 2462 AD - International Joint Research Center of Green Energy Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China. RINGGOLD: 47860 FAU - Li, Jinhong AU - Li J AD - Department of Chemistry, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, United States. RINGGOLD: 2462 FAU - Wang, Zitong AU - Wang Z AUID- ORCID: 0000-0001-7883-5276 AD - Department of Chemistry, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, United States. RINGGOLD: 2462 FAU - Mao, Yibin AU - Mao Y AD - Department of Chemistry, The University of Chicago, 929 East 57th Street, Chicago, Illinois 60637, United States. RINGGOLD: 2462 FAU - Shi, Li AU - Shi L AUID- ORCID: 0000-0002-2998-3384 AD - International Joint Research Center of Green Energy Chemical Engineering, East China University of Science and Technology, Shanghai 200237, China. RINGGOLD: 47860 FAU - Lin, Wenbin AU - Lin W AUID- ORCID: 0000-0001-7035-7759 AD - Department of Chemistry, Westlake University, 600 Dunyu Road, Hangzhou, Zhejiang 310030, China. RINGGOLD: 557712 LA - eng PT - Journal Article DEP - 20260430 PL - United States TA - Precis Chem JT - Precision chemistry JID - 9918574281706676 PMC - PMC13715352 OTO - NOTNLM OT - catalytic unit OT - integration OT - metal-organic framework OT - photocatalytic CO2 reduction OT - photosensitizer EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/04/30 CRDT- 2026/10/29 06:11 PHST- 2026/01/25 00:00 [received] PHST- 2026/03/19 00:00 [revised] PHST- 2026/03/19 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:11 [entrez] PHST- 2026/04/30 00:00 [pmc-release] AID - 10.1021/prechem.6c00014 [doi] PST - epublish SO - Precis Chem. 2026 Apr 30;4(10):1639-1644. doi: 10.1021/prechem.6c00014. eCollection 2026 Oct 26. PMID- 42953687 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2832-3637 (Electronic) IS - 2832-3637 (Linking) VI - 4 IP - 10 DP - 2026 Oct 26 TI - Benchmarking Machine Learning and Automated Image Analysis for Organelle Quantification. PG - 2226-2245 LID - 10.1021/cbmi.6c00038 [doi] AB - Quantitative organelle analysis is highly sensitive to image-processing choices, limiting reproducibility across microscopy studies. Here, we systematically compare automated, interactive machine learning, and deep learning-based pipelines for lipid droplet and mitochondrial quantification in live human osteosarcoma cells imaged by fluorescence microscopy and label-free holotomography. Using standardized downstream feature extraction, we evaluated script-based workflows (Fiji, Python), a modular platform (CellProfiler), interactive machine learning (ilastik), and pretrained deep learning models. Lipid droplet segmentation was qualitatively consistent across approaches; however, droplet counts, and size distributions varied substantially between pipelines and imaging modalities, with ilastik reducing background-driven detections and improving cross-modality agreement. In contrast, mitochondrial quantification proved highly sensitive to segmentation and skeletonization choices, particularly in holotomography where global intensity-threshold-based methods failed to capture network structure. Based on these cross-pipeline comparisons, we demonstrate how organelle- and modality-specific benchmarking can guide pipeline selection, illustrated by the analysis of metabolic perturbations affecting lipid droplets and mitochondria. Together, these results highlight modality- and morphology-dependent limitations in common analysis pipelines and provide practical guidance for selecting robust, reproducible strategies for quantitative organelle imaging. CI - (c) 2026 The Authors. Co-published by Nanjing University and American Chemical Society. FAU - Daul, Chloe AU - Daul C AD - Department of Biomedical Sciences, University of Lausanne, Lausanne 1005, Switzerland. RINGGOLD: 27213 AD - Ecole Polytechnique Federale de Lausanne (EPFL), Lausanne 1015, Switzerland. FAU - Tournier, Pierre AU - Tournier P AD - Department of Biomedical Sciences, University of Lausanne, Lausanne 1005, Switzerland. RINGGOLD: 27213 FAU - Habib, Shukry J AU - Habib SJ AUID- ORCID: 0000-0003-3132-2216 AD - Department of Biomedical Sciences, University of Lausanne, Lausanne 1005, Switzerland. RINGGOLD: 27213 LA - eng PT - Journal Article DEP - 20260508 PL - United States TA - Chem Biomed Imaging JT - Chemical & biomedical imaging JID - 9918574388106676 PMC - PMC13715356 OTO - NOTNLM OT - fluorescence microscopy OT - holotomography OT - image analysis pipelines OT - lipid droplets OT - machine learning segmentation OT - mitochondria OT - organelle quantification EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/05/08 CRDT- 2026/10/29 06:11 PHST- 2026/02/10 00:00 [received] PHST- 2026/04/08 00:00 [revised] PHST- 2026/04/20 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:11 [entrez] PHST- 2026/05/08 00:00 [pmc-release] AID - 10.1021/cbmi.6c00038 [doi] PST - epublish SO - Chem Biomed Imaging. 2026 May 8;4(10):2226-2245. doi: 10.1021/cbmi.6c00038. eCollection 2026 Oct 26. PMID- 42953685 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2168-8184 (Print) IS - 2168-8184 (Electronic) IS - 2168-8184 (Linking) VI - 18 IP - 9 DP - 2026 Sep TI - Reproductive and Gynecologic Effects of Glucagon-Like Peptide-1 Receptor Agonists in Women With Polycystic Ovary Syndrome: A Scoping Review. PG - e117072 LID - 10.7759/cureus.117072 [doi] LID - e117072 AB - Polycystic ovary syndrome (PCOS) is a common endocrine disorder characterized by metabolic dysfunction, hyperandrogenism, ovulatory dysfunction, and infertility. Although glucagon-like peptide-1 receptor agonists (GLP-1 RAs) have demonstrated efficacy in promoting weight loss and improving insulin resistance, their effects on reproductive and gynecologic outcomes in women with PCOS have not been comprehensively summarized. This scoping review aimed to evaluate the available evidence regarding the reproductive and gynecologic outcomes associated with GLP-1 RA therapy in women with PCOS. A comprehensive literature search was conducted in PubMed, Embase, Web of Science, and Nursing & Allied Health Premium. Original studies evaluating GLP-1 RA therapy in women with PCOS and reporting reproductive or gynecologic outcomes were eligible for inclusion. Following duplicate removal, studies underwent title/abstract screening, full-text review, and standardized data extraction. Outcomes of interest included menstrual function, ovulation, fertility and pregnancy outcomes, hyperandrogenism, reproductive hormone profiles, ovarian function, and secondary metabolic outcomes. Twenty-eight studies met the inclusion criteria. Across the available literature, GLP-1 RA therapy was consistently associated with improvements in biochemical markers of hyperandrogenism, including reductions in total testosterone, free testosterone, free androgen index, and androstenedione, and increases in sex hormone-binding globulin. Improvements in menstrual regularity and reductions in amenorrhea were also frequently reported. Evidence regarding ovulation, fertility, and pregnancy outcomes was more limited but generally suggested improved ovulation rates and increased pregnancy rates in selected populations, particularly when GLP-1 RAs were combined with metformin. Nearly all included studies also demonstrated significant improvements in body weight, body mass index, and insulin resistance. Considerable heterogeneity in study design, interventions, and reported outcomes precluded quantitative synthesis. The overall findings suggest that GLP-1 RAs may provide reproductive and gynecologic benefits in women with PCOS in addition to their established metabolic effects. The most consistent improvements were observed in hyperandrogenism and menstrual function, whereas evidence supporting effects on fertility and pregnancy outcomes remains comparatively limited. Further high-quality prospective studies with standardized reproductive outcome measures are needed to better define the role of GLP-1 RAs in the management of PCOS. CI - Copyright (c) 2026, Snytte et al. FAU - Snytte, Jacklyn AU - Snytte J AD - Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Davie, USA. FAU - Hazan, Brielle AU - Hazan B AD - Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Davie, USA. FAU - Weissman, Amanda AU - Weissman A AD - Dr. Kiran C. Patel College of Osteopathic Medicine, Nova Southeastern University, Davie, USA. FAU - Mayrovitz, Harvey N AU - Mayrovitz HN AD - Medical Education, Dr. Kiran C. Patel College of Allopathic Medicine, Nova Southeastern University, Davie, USA. LA - eng PT - Journal Article PT - Review DEP - 20260928 PL - United States TA - Cureus JT - Cureus JID - 101596737 PMC - PMC13714954 OTO - NOTNLM OT - exenatide OT - fertility OT - glp-1 receptor agonists OT - hyperandrogenism OT - insulin resistance OT - liraglutide OT - menstrual irregularity OT - obesity OT - polycystic ovary syndrome OT - semaglutide COIS- Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/28 CRDT- 2026/10/29 06:11 PHST- 2026/08/06 00:00 [received] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:11 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - 10.7759/cureus.117072 [doi] PST - epublish SO - Cureus. 2026 Sep 28;18(9):e117072. doi: 10.7759/cureus.117072. eCollection 2026 Sep. PMID- 42953668 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2168-8184 (Print) IS - 2168-8184 (Electronic) IS - 2168-8184 (Linking) VI - 18 IP - 9 DP - 2026 Sep TI - Carbohydrate Quality Versus Quantity in Gestational Diabetes Mellitus While Considering Randomized Dietary Interventions: A Systematic Review. PG - e117076 LID - 10.7759/cureus.117076 [doi] LID - e117076 AB - Medical nutrition therapy is the first-line treatment for gestational diabetes mellitus (GDM), but whether carbohydrate quality or quantity should be prioritized is unclear, as dietary interventions often change amount, glycemic quality, fiber, fat, protein, and food pattern together. We systematically reviewed randomized trials of carbohydrate quality or quantity in diagnosed GDM. PubMed and the Cochrane Central Register of Controlled Trials (CENTRAL) were searched from inception to July 19, 2026, with citation searching. Two human reviewers screened the records and extracted the data with artificial-intelligence assistance. Risk of bias was assessed using the revised Cochrane risk-of-bias tool (RoB 2), and the findings were primarily synthesized narratively using the Synthesis Without Meta-analysis (SWiM) approach, with limited random-effects meta-analysis for clinically compatible insulin-related outcomes. From 981 records, 34 reports representing 25 independent randomized studies were included; they differed markedly in construct, achieved carbohydrate separation, rescue treatment, and analysis population. In an exploratory, treatment-threshold analysis, lower-glycemic-index assignment reduced the proportion meeting insulin-start criteria (two trials, 159 participants; risk ratio [RR] 0.43, 95% confidence interval [CI] 0.25-0.75), but rescue switching removed the difference in actual insulin use. Lower-carbohydrate assignment did not reduce insulin initiation (three trials, 248 participants; RR 1.02, 95% CI 0.76-1.35). This finding remained essentially unchanged after excluding the trial in which the intended difference in carbohydrate intake was not achieved. With only two or three small trials per pool, I(2) = 0% does not indicate homogeneity. Fiber, staple-quality, and provided-diet interventions improved selected postprandial measures, but obstetric, birth-size, and neonatal-adiposity benefits were not established, and certainty was low or very low. Carbohydrate quality is a plausible route to postprandial improvement without aggressive restriction, but current randomized evidence does not establish a universal quality-over-quantity rule or support lower carbohydrate intake as superior. Carbohydrate amount should be individualized while prioritizing nutrient-dense, lower-glycemic-index, fiber-rich sources. CI - Copyright (c) 2026, Aggarwal et al. FAU - Aggarwal, Swati AU - Aggarwal S AD - Nutrition and Dietetics, Manav Rachna International Institute of Research and Studies, Faridabad, IND. FAU - Chawla, Gurjeet K AU - Chawla GK AD - Nutrition and Dietetics, Manav Rachna International Institute of Research and Studies, Faridabad, IND. FAU - Gupta, Renu AU - Gupta R AD - Gynecology and Obstetrics, Sri Balaji Action Medical Institute, New Delhi, IND. LA - eng PT - Journal Article PT - Review DEP - 20260928 PL - United States TA - Cureus JT - Cureus JID - 101596737 PMC - PMC13714952 OTO - NOTNLM OT - carbohydrate OT - dietary fiber OT - gestational diabetes mellitus OT - glycemic index OT - systematic review COIS- Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/28 CRDT- 2026/10/29 06:10 PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:10 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - 10.7759/cureus.117076 [doi] PST - epublish SO - Cureus. 2026 Sep 28;18(9):e117076. doi: 10.7759/cureus.117076. eCollection 2026 Sep. PMID- 42953665 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2624-9367 (Electronic) IS - 2624-9367 (Linking) VI - 8 DP - 2026 TI - Similar perceived availability but lower use of physical activity spaces among Chilean adults with disabilities. PG - 1969080 LID - 10.3389/fspor.2026.1969080 [doi] LID - 1969080 AB - BACKGROUND: Adults with disabilities are less physically active than the general population, yet the environmental point at which this disparity emerges is rarely identified in national surveillance. We examined perceived availability, use, and barriers to the use of physical activity spaces among Chilean adults with and without disabilities, and estimated the association of disability with physical inactivity and with non-use of available spaces. METHODS: Cross-sectional analysis of the 2024 National Survey of Physical Activity and Sports Habits (ENAFyD), a probabilistic national sample of adults in Chile (n = 8,653). Self-reported disability was classified into mutually exclusive categories (sensory only, physical only, cognitive only, multiple types, other only, none) and cross-classified with perceived availability of spaces near the home, their use, reported barriers among non-users, and the survey physical activity index. Group differences were tested with weighted Poisson regression with robust variance, reported as crude prevalence ratios (PR) with 95% confidence intervals and p values; adjusted models included sex, age group, and socioeconomic status, selected a priori as confounders using a directed acyclic graph. RESULTS: The weighted prevalence of disability was 9.3%. Perceived availability of spaces did not differ between groups (92.1% vs. 91.4%; crude PR 1.01; 95% CI 0.99-1.03; p = 0.478). However, among adults with available spaces, only 29.9% (95% CI 25.4-34.8) of those with disabilities used them, compared with 42.7% (40.8-44.6) of those without (crude PR for non-use 1.22; 1.16-1.29; p < 0.001). The largest differential barriers were distance or difficulty of access (14.0% vs. 7.7%; PR 1.82; 1.44-2.31) and lack of companionship (25.9% vs. 16.8%; PR 1.54; 1.31-1.80). In adjusted models, disability was associated with physical inactivity (PR 1.22; 1.11-1.33) and with non-use of available spaces (PR 1.14; 1.08-1.20), with the largest estimate among adults reporting multiple disability types (PR 1.47; 1.35-1.60). CONCLUSIONS: In Chilean adults, the disability gap in physical activity is concentrated in the use of spaces rather than in their perceived availability, and access- and companionship-related barriers were more frequently reported by non-users with disabilities. Policies should prioritize the usability of existing spaces, through accessibility, safe access, and social support, alongside new construction. CI - (c) 2026 De Souza-Lima, Winckler, Olasagasti-Ibargoien, Yanez-Sepulveda, Godoy-Tapia, Parra-Saldias, Duclos-Bastias, Godoy-Cumillaf, Merellano-Navarro, Bruneau-Chavez, Reyes-Amigo and Farias-Valenzuela. FAU - De Souza-Lima, Josivaldo AU - De Souza-Lima J AD - Facultad de Educacion y Humanidades, Escuela de Ciencias del Deporte, Universidad Andres Bello, Santiago, Chile. FAU - Winckler, Ciro AU - Winckler C AD - Programa de Pos-Graduacao em Ciencias do Movimento Humano e Reabilitacao, Universidade Federal de Sao Paulo (UNIFESP), Santos, Sao Paulo, Brazil. FAU - Olasagasti-Ibargoien, Jurgi AU - Olasagasti-Ibargoien J AD - Health, Physical Activity and Sports Science Laboratory, Department of Physical Activity and Sports, Faculty of Education and Sport, University of Deusto, Bilbao, Spain. FAU - Yanez-Sepulveda, Rodrigo AU - Yanez-Sepulveda R AD - Facultad de Educacion y Humanidades, Escuela de Ciencias del Deporte, Universidad Andres Bello, Santiago, Chile. AD - Universidad Espiritu Santo, Samborondon, Ecuador. FAU - Godoy-Tapia, Cristian AU - Godoy-Tapia C AD - Facultad de Educacion, Carrera de Educacion Fisica, Universidad Adventista de Chile, Chillan, Chile. FAU - Parra-Saldias, Maribel AU - Parra-Saldias M AD - Departamento de Educacion Fisica, Deporte y Recreacion, Universidad de Atacama, Copiapo, Chile. FAU - Duclos-Bastias, Daniel AU - Duclos-Bastias D AD - iGEO Research, Escuela de Educacion Fisica, Pontificia Universidad Catolica de Valparaiso, Valparaiso, Chile. AD - METIS Research Group, Facultad de Negocios y Tecnologia, Universidad Alfonso X el Sabio (UAX), Madrid, Spain. FAU - Godoy-Cumillaf, Andres AU - Godoy-Cumillaf A AD - Grupo de Investigacion en Educacion Fisica, Salud y Calidad de Vida (EFISAL), Facultad de Educacion, Universidad Autonoma de Chile, Temuco, Chile. FAU - Merellano-Navarro, Eugenio AU - Merellano-Navarro E AD - Department of Physical Activity Sciences, Faculty of Education Sciences, Universidad Catolica del Maule, Talca, Chile. FAU - Bruneau-Chavez, Jose AU - Bruneau-Chavez J AD - Departamento de Educacion Fisica, Deportes y Recreacion, Universidad de La Frontera, Temuco, Chile. FAU - Reyes-Amigo, Tomas AU - Reyes-Amigo T AD - Physical Activity Sciences Observatory (OCAF), Universidad de Playa Ancha, Valparaiso, Chile. FAU - Farias-Valenzuela, Claudio AU - Farias-Valenzuela C AD - Escuela de Ciencias de la Actividad Fisica, Universidad de Las Americas, Santiago, Chile. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Sports Act Living JT - Frontiers in sports and active living JID - 101765780 PMC - PMC13714973 OTO - NOTNLM OT - accessibility OT - built environment OT - disability OT - national survey OT - physical activity COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 06:10 PHST- 2026/08/15 00:00 [received] PHST- 2026/09/01 00:00 [revised] PHST- 2026/09/04 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:10 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fspor.2026.1969080 [doi] PST - epublish SO - Front Sports Act Living. 2026 Oct 14;8:1969080. doi: 10.3389/fspor.2026.1969080. eCollection 2026. PMID- 42953645 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Case Report: transient migratory pulmonary nodules with bronchial artery malformations in an autosomal dominant polycystic kidney disease patient: a case of recurrent hemoptysis. PG - 1949035 LID - 10.3389/fmed.2026.1949035 [doi] LID - 1949035 AB - Autosomal dominant polycystic kidney disease (ADPKD) is associated with vascular malformations, but bronchial artery involvement with migratory pulmonary nodules has not been reported. We describe an 81-year-old man with recurrent hemoptysis and migratory nodules on chest CT. Digital subtraction angiography revealed tortuous, dilated bronchial arteries; embolization controlled bleeding and nodules resolved. He had polycystic kidney and liver disease, a prior intracranial aneurysm, and a PKD1 missense variant (c.8426C > T). Common causes of hemoptysis were excluded. This case describes co-occurrence of bronchial artery malformation, recurrent hemoptysis and migratory pulmonary nodules in a patient with genetically confirmed ADPKD, raising the possibility that bronchial arterial involvement could be a rare, under-recognized vascular manifestation associated with ADPKD. CI - (c) 2026 Hu, Zhang, Wang and Xue. FAU - Hu, Yi AU - Hu Y AD - Department of Respiratory and Critical Care Medicine, Chuiyangliu Hospital Affiliated with Tsinghua University, Beijing, China. FAU - Zhang, Jie AU - Zhang J AD - Department of Respiratory and Critical Care Medicine, Chuiyangliu Hospital Affiliated with Tsinghua University, Beijing, China. FAU - Wang, Ting AU - Wang T AD - Department of Respiratory and Critical Care Medicine, Chuiyangliu Hospital Affiliated with Tsinghua University, Beijing, China. FAU - Xue, Bing AU - Xue B AD - Department of Respiratory and Critical Care Medicine, Chuiyangliu Hospital Affiliated with Tsinghua University, Beijing, China. LA - eng PT - Case Reports PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13714881 OTO - NOTNLM OT - autosomal dominant polycystic kidney disease OT - bronchial artery malformation OT - hemoptysis OT - polycystic kidney OT - pulmonary nodule COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 06:09 PHST- 2026/07/26 00:00 [received] PHST- 2026/08/30 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:09 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmed.2026.1949035 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 14;13:1949035. doi: 10.3389/fmed.2026.1949035. eCollection 2026. PMID- 42953638 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2168-8184 (Print) IS - 2168-8184 (Electronic) IS - 2168-8184 (Linking) VI - 18 IP - 9 DP - 2026 Sep TI - Multidisciplinary Antimicrobial Stewardship Program in an Indian ICU: A Time-Series Analysis of Diagnostic Practices and Antibiotic Use. PG - e117062 LID - 10.7759/cureus.117062 [doi] LID - e117062 AB - Background ICUs are high-risk environments for antimicrobial resistance (AMR). We evaluated the impact of a multidisciplinary Antimicrobial Stewardship Program (AMSP) on diagnostic stewardship, prescribing patterns, and antimicrobial consumption in an Indian tertiary critical care setting. Methods A 17-month retrospective observational study was conducted, comparing an eight-month pre-implementation phase (January-August 2024) with a nine-month post-implementation phase (September 2024-May 2025). The AMSP included staff training, structured prescription orders, prospective audits, and monthly feedback. Key metrics included pre-antibiotic culture collection, institutional guideline adherence, surgical antimicrobial prophylaxis (SAP) compliance, and standardized consumption densities (defined daily dose (DDD)/100 bed-days and days of therapy (DOT)/1,000 bed-days), evaluated using interrupted time-series (ITS) regression. Results Across 7,682 ICU admissions (3,514 pre- vs. 4,168 post-implementation), pre-antibiotic culture collection increased significantly: blood cultures increased from 331 (20.2%) to 560 (45.8%; p < 0.001), and urine cultures increased from 1,365 (53.0%) to 1,583 (64.6%; p < 0.001). Empirical guideline adherence improved from 1,618 (46.0%) to 3,005 (72.1%; p < 0.001), and SAP compliance increased from 1,352 (35.5%) to 2,083 (63.2%; p < 0.001). Total antimicrobial consumption density decreased by 44.0% (120.04 to 67.17 DDD/100 bed-days; p < 0.001), and duration density declined by 39.6% (981.23 to 592.91 DOT/1,000 bed-days; p < 0.001; ITS regression step-reduction p < 0.001). Meropenem exposure remained strictly controlled and stable (165 (4.7%) vs. 208 (5.0%); p = 0.549). Conclusion The multidisciplinary AMSP successfully contained overall antibiotic selection pressure while preserving carbapenems through optimized beta-lactam/beta-lactamase inhibitor utilization. Microbial ecology remained stable, with Klebsiella spp. and Escherichia coli predominating. Unadjusted case-mix differences represent a recognized study limitation. Implementing a multidisciplinary AMSP achieved sustained reductions in antimicrobial consumption and improved diagnostic practices, providing a feasible framework to combat resistance in resource-limited ICUs. CI - Copyright (c) 2026, Shaik et al. FAU - Shaik, Naseema AU - Shaik N AD - Microbiology, NRI Medical College and General Hospital, Guntur, IND. FAU - Simgamsetty, Susmitha AU - Simgamsetty S AD - Microbiology, Saveetha Medical College and Hospital, Chennai, IND. FAU - Yarlagadda, Padmaja AU - Yarlagadda P AD - Microbiology, NRI Medical College and General Hospital, Guntur, IND. FAU - Penmethsa, Uma AU - Penmethsa U AD - Microbiology, NRI Medical College and General Hospital, Guntur, IND. FAU - Dharmalingam Jothinathan, Mukesh Kumar AU - Dharmalingam Jothinathan MK AD - Centre for Global Health Research, Saveetha Medical College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai, IND. LA - eng PT - Journal Article DEP - 20260928 PL - United States TA - Cureus JT - Cureus JID - 101596737 PMC - PMC13714860 OTO - NOTNLM OT - antibiotic consumption OT - antimicrobial resistance OT - antimicrobial stewardship OT - de-escalation OT - diagnostic stewardship OT - india OT - intensive care unit OT - time-series analysis COIS- Human subjects: Informed consent for treatment and open access publication was obtained or waived by all participants in this study. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/28 CRDT- 2026/10/29 06:08 PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:08 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - 10.7759/cureus.117062 [doi] PST - epublish SO - Cureus. 2026 Sep 28;18(9):e117062. doi: 10.7759/cureus.117062. eCollection 2026 Sep. PMID- 42953629 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2168-8184 (Print) IS - 2168-8184 (Electronic) IS - 2168-8184 (Linking) VI - 18 IP - 9 DP - 2026 Sep TI - Severe and Prolonged Paroxetine Intoxication With Delayed Absorption and Extreme Serum Concentrations: A Case Report. PG - e117066 LID - 10.7759/cureus.117066 [doi] LID - e117066 AB - Selective serotonin reuptake inhibitors (SSRIs) are commonly prescribed antidepressants, known for their relatively safe pharmacological profile and mild symptoms in cases of overdose compared with other antidepressants. In this case report, we describe a near-fatal case of a 70-year-old woman who ingested multiple drugs with suicidal intent, including an unknown quantity of celecoxib 200 mg, clorazepate acid 5 mg, omeprazole 20 mg, and immediate-release paroxetine 30 mg tablets. The patient developed severe serotonin syndrome complicated by acute respiratory distress syndrome (ARDS) and required admission to the intensive care unit (ICU). Because of progressive respiratory deterioration, she required intubation and invasive mechanical ventilation. Serotonin syndrome persisted for an unusually long period, with serum paroxetine concentrations of 4805 microg/L at admission that continued to rise to a maximum of 7824 microg/L on day four, both well above the lethal threshold. Intensive therapeutic interventions aimed at lowering paroxetine concentrations and resolving serotonin syndrome included activated charcoal, whole-bowel irrigation lavage, and rifampicin. Paroxetine concentrations gradually declined and reached the therapeutic range after 16 days. Following further ICU treatment, including prone ventilation and corticosteroids, the ARDS resolved, and the patient was extubated 21 days after ingestion. This case highlights that paroxetine overdose may present more severely than commonly reported in the literature, with delayed and prolonged toxicity, including serotonin syndrome and ARDS. Paroxetine overdose may be life-threatening and underscores the need for close monitoring of serum paroxetine concentrations and timely consideration of activated charcoal and whole-bowel irrigation. CI - Copyright (c) 2026, Beijnsberger et al. FAU - Beijnsberger, Luna AU - Beijnsberger L AD - Intensive Care Medicine, Elisabeth-TweeSteden Ziekenhuis, Tilburg, NLD. FAU - Boly, Chantal A AU - Boly CA AD - Intensive Care Medicine, Elisabeth-TweeSteden Ziekenhuis, Tilburg, NLD. FAU - Jaspers, Tessa C AU - Jaspers TC AD - Clinical Pharmacy, Elisabeth-TweeSteden Ziekenhuis, Tilburg, NLD. FAU - Van Oers, Jos A AU - Van Oers JA AD - Intensive Care Medicine, Elisabeth-TweeSteden Ziekenhuis, Tilburg, NLD. LA - eng PT - Case Reports PT - Journal Article DEP - 20260928 PL - United States TA - Cureus JT - Cureus JID - 101596737 PMC - PMC13714862 OTO - NOTNLM OT - acute respiratory distress syndrome (ards) OT - clinical pharmacokinetics OT - drug-induced toxicity OT - paroxetine OT - serotonin syndrome (ss) COIS- Human subjects: Informed consent for treatment and open access publication was obtained or waived by all participants in this study. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/28 CRDT- 2026/10/29 06:07 PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:07 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - 10.7759/cureus.117066 [doi] PST - epublish SO - Cureus. 2026 Sep 28;18(9):e117066. doi: 10.7759/cureus.117066. eCollection 2026 Sep. PMID- 42953627 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2691-3704 (Electronic) IS - 2691-3704 (Linking) VI - 6 IP - 10 DP - 2026 Oct 26 TI - Unlocking Iridium Chemical Space for Antibiotic Discovery through One-Pot Combinatorial Synthesis in a Direct-to-Biology Approach. PG - 5605-5622 LID - 10.1021/jacsau.6c00813 [doi] AB - Antibacterial resistance continues to drive the need for exploring novel chemical spaces beyond conventional organic scaffolds. Metal-based compounds have emerged as a promising class of antibiotic candidates, demonstrating high hit rates against priority bacterial pathogens without exhibiting increased toxicity compared to purely organic molecules. Here, we report a one-pot, three-component combinatorial strategy for the synthesis of piano-stool Ir-(III) cyclopentadienyl Schiff-base complexes as potential antibacterial agents. Using 15 picolinaldehydes (A-O), 24 amines (1-24), and four Cp/Cp* iridium precursors (Cp1-4), a library of 1440 complexes was generated and screened directly from crude reaction mixtures in a direct-to-biology approach. Biological screening revealed pronounced activity against Gram-positive Staphylococcus aureus, particularly for biphenyl-substituted Cp (Cp2) complexes, while no activity was observed against Gram-negative bacteria. A subset of the compounds exhibited potent and selective antibacterial activity with low mammalian cell toxicity. Dose-response validation identified 4 promising hit candidates, of which Ir15 displayed low-micromolar activity against a panel of Gram-positive pathogens, limited hemolysis, and moderate cytotoxicity. Initial mechanistic studies indicate that Ir15 acts through a membrane-associated mode of action involving mild membrane depolarization without large pore formation, alongside changes in nucleoid morphology. Overall, this work establishes Ir-(III)-Cp Schiff-base complexes as a modular antibacterial platform and identifies Ir15 as a promising lead compound for further optimization. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Ozsan, Cagri AU - Ozsan C AD - Department of Chemistry, Biochemistry & Pharmaceutical Sciences, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland. RINGGOLD: 27210 AD - Department of Chemistry, University of York, York YO10 5DD, U.K. RINGGOLD: 8748 FAU - Welsh, Athi AU - Welsh A AD - Department of Chemistry, Biochemistry & Pharmaceutical Sciences, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland. RINGGOLD: 27210 AD - Department of Chemistry, University of York, York YO10 5DD, U.K. RINGGOLD: 8748 FAU - Zineddu, Stefano AU - Zineddu S AD - Department of Chemistry "Ugo Schiff", University of Florence, Sesto Fiorentino 50019, Italy. FAU - Husbands, David R AU - Husbands DR AUID- ORCID: 0000-0001-8693-7766 AD - Department of Chemistry, University of York, York YO10 5DD, U.K. RINGGOLD: 8748 FAU - Robinson, Kiera AU - Robinson K AD - Department of Chemistry, University of York, York YO10 5DD, U.K. RINGGOLD: 8748 FAU - Chau, Anson Kwok-Hei AU - Chau AK AD - Department of Chemistry, University of York, York YO10 5DD, U.K. RINGGOLD: 8748 FAU - Frei, Angelo AU - Frei A AUID- ORCID: 0000-0001-6169-2491 AD - Department of Chemistry, Biochemistry & Pharmaceutical Sciences, University of Bern, Freiestrasse 3, 3012 Bern, Switzerland. RINGGOLD: 27210 AD - Department of Chemistry, University of York, York YO10 5DD, U.K. RINGGOLD: 8748 LA - eng PT - Journal Article DEP - 20260918 PL - United States TA - JACS Au JT - JACS Au JID - 101775714 PMC - PMC13714831 OTO - NOTNLM OT - Schiff-base OT - antibacterial OT - combinatorial synthesis OT - direct-to-biology OT - iridium OT - metalloantibiotics EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/18 CRDT- 2026/10/29 06:07 PHST- 2026/05/25 00:00 [received] PHST- 2026/09/01 00:00 [revised] PHST- 2026/09/02 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:07 [entrez] PHST- 2026/09/18 00:00 [pmc-release] AID - 10.1021/jacsau.6c00813 [doi] PST - epublish SO - JACS Au. 2026 Sep 18;6(10):5605-5622. doi: 10.1021/jacsau.6c00813. eCollection 2026 Oct 26. PMID- 42953624 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2691-3704 (Electronic) IS - 2691-3704 (Linking) VI - 6 IP - 10 DP - 2026 Oct 26 TI - Reengineered Vitamin B12 for Selective Aqueous Nitrate-to-Ammonium Electrocatalysis via Axial-Ligand Gating. PG - 5711-5721 LID - 10.1021/jacsau.6c00970 [doi] AB - Vitamin B12 (cobalamin) is an evolutionarily optimized Co-corrin redox cofactor capable of accessing low-valent cobalt states to mediate challenging biological transformations. Here, we reengineer this biological redox platform into an electrode-addressable catalyst for aqueous nitrate-to-ammonium electrocatalysis and demonstrate that catalytic selectivity is governed by the cooperative interplay between axial-ligand electronic tuning and local proton management. An isostructural series of lower-axial variants (C1-C4) was prepared by systematically modifying the native dimethylbenzimidazole environment, culminating in an l-histidine-ligated derivative (C4) bearing proximal -COOH/-NH(2) functionality. In mildly acidic MOPS buffer (pH 5.81), C4 exhibited the highest performance, achieving approximately 90% NH(4) (+) Faradaic efficiency at -1.1 V vs SCE while effectively suppressing H(2) and hydroxylamine formation compared with the other derivatives. Electrochemical studies reveal that axial-ligand engineering modulates the Co-(III)/Co-(I) redox manifold, facilitating access to the catalytically active Co-(I) state and promoting nitrate activation. Mechanistic investigations support a pathway in which electrochemical reduction induces Co-CN bond dissociation and solvent coordination to generate a Co-(I)-solvent intermediate, followed by nitrate binding and proton-coupled electron transfer through nitrite and hydroxylamine intermediates to ammonium. (15)N isotopic labeling and in situ spectroelectrochemical measurements confirm the formation of nitrate-derived NH(4) (+) and validate the proposed catalytic intermediates. To evaluate the transferability of this molecular design, the catalysts were immobilized on graphene oxide. The heterogeneous systems preserved the activity trend observed in solution, with C4@GO exhibiting the highest NH(4) (+) selectivity, demonstrating that the electronic effects imparted by lower axial-ligand engineering remain operative after immobilization. Together, these results establish vitamin B12 as a versatile Co-corrin platform and identify axial-ligand-controlled electronic tuning coupled with spatially programmed proton delivery as a general strategy for designing efficient molecular and hybrid electrocatalysts for selective multiproton, multielectron nitrate reduction in water. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Das, Chandan AU - Das C AD - Chemistry Department, Indian Institute of Technology Bombay, Mumbai 400076, India. RINGGOLD: 29491 FAU - Ghosh, Subho AU - Ghosh S AD - Chemistry Department, Indian Institute of Technology Bombay, Mumbai 400076, India. RINGGOLD: 29491 FAU - Biswas, Rathindranath AU - Biswas R AUID- ORCID: 0000-0002-0909-1442 AD - Chemistry Department, Indian Institute of Technology Bombay, Mumbai 400076, India. RINGGOLD: 29491 FAU - Guha, Aritra AU - Guha A AD - Chemistry Department, Indian Institute of Technology Bombay, Mumbai 400076, India. RINGGOLD: 29491 FAU - Majumder, Piyali AU - Majumder P AD - Department of Life Science, Ramnarain Ruia Autonomous College, Matunga (East), Mumbai 400019, India. FAU - Lahiri, Goutam Kumar AU - Lahiri GK AUID- ORCID: 0000-0002-0199-6132 AD - Chemistry Department, Indian Institute of Technology Bombay, Mumbai 400076, India. RINGGOLD: 29491 AD - Department of Chemical Sciences, Indian Institute of Science Education and Research, Mohanpur, Kolkata 741246, India. FAU - Dutta, Arnab AU - Dutta A AUID- ORCID: 0000-0002-9998-6329 AD - Chemistry Department, Indian Institute of Technology Bombay, Mumbai 400076, India. RINGGOLD: 29491 AD - National Centre of Excellence in Carbon Capture, Utilization, and Storage (NCoE-CCUS), Indian Institute of Technology Bombay, Mumbai 400076, India. AD - Centre for Climate Studies, Indian Institute of Technology Bombay, Mumbai 400076, India. LA - eng PT - Journal Article DEP - 20260921 PL - United States TA - JACS Au JT - JACS Au JID - 101775714 PMC - PMC13714829 OTO - NOTNLM OT - Ammonia electrosynthesis OT - Axial-ligand engineering OT - Bioinspired electrocatalysis OT - Molecular homo- and heterogeneous catalysis OT - Nitrate reduction OT - Proton-coupled electron transfer OT - Vitamin B12 derivatives EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/21 CRDT- 2026/10/29 06:07 PHST- 2026/06/20 00:00 [received] PHST- 2026/08/06 00:00 [revised] PHST- 2026/08/07 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:07 [entrez] PHST- 2026/09/21 00:00 [pmc-release] AID - 10.1021/jacsau.6c00970 [doi] PST - epublish SO - JACS Au. 2026 Sep 21;6(10):5711-5721. doi: 10.1021/jacsau.6c00970. eCollection 2026 Oct 26. PMID- 42953621 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2234-943X (Print) IS - 2234-943X (Electronic) IS - 2234-943X (Linking) VI - 16 DP - 2026 TI - Integrated bioinformatics and machine learning identify key hub genes in early-onset gastric cancer. PG - 1951255 LID - 10.3389/fonc.2026.1951255 [doi] LID - 1951255 AB - BACKGROUND: Early-onset gastric cancer (EOGC) represents a clinically and biologically distinct subtype of gastric cancer characterized by unique molecular features. However, its underlying molecular mechanisms remain unclear. This study aimed to identify EOGC-associated hub genes using an integrated bioinformatics and machine learning framework. METHODS: Differentially expressed genes (DEGs) between EOGC and late-onset gastric cancer (LOGC) were analyzed using functional enrichment, weighted gene co-expression network analysis (WGCNA), least absolute shrinkage and selection operator (LASSO) regression, and random forest algorithms. Immune infiltration was characterized using CIBERSORT. Candidate hub genes were validated using an independent external cohort, clinical specimens, single-cell transcriptomic datasets, and in vitro functional assays. A five-gene logistic regression model was established and evaluated using ROC-AUC, calibration analysis, and bootstrap resampling. RESULTS: Transcriptomic analysis identified 227 DEGs, and WGCNA identified seven co-expression modules, with the green module showing the strongest association with EOGC. Machine learning identified five hub genes: CCR7, CD1D, PTGER2, PIK3CG, and EBI3. The combined model showed good discriminatory performance in the training, internal validation, and independent external validation cohorts (AUC = 0.909, 0.859, and 0.802, respectively). Bootstrap resampling yielded AUC estimates of 0.908, 0.852, and 0.789, respectively, supporting model stability. All five genes showed higher expression in EOGC and were validated at the mRNA and protein levels. Immune profiling and single-cell analysis revealed an immune-associated molecular phenotype and distinct expression patterns across immune cell subsets. Silencing the hub genes suppressed gastric cancer cell proliferation and migration in vitro. CONCLUSION: We identified five EOGC-associated hub genes and revealed an immune-associated molecular landscape. The five-gene model represents an EOGC-associated molecular signature distinguishing EOGC from LOGC rather than a stand-alone clinical diagnostic tool. These findings provide insights into the molecular and immune biology of EOGC and may inform future studies of molecular stratification and treatment-related outcomes. CI - Copyright (c) 2026 Yi, Chen, Wu, Hou, Chen, Zhang, Zhu, Liu, Liang, Zhang and Yang. FAU - Yi, Shaoxiong AU - Yi S AD - Digestive Medicine Center, the Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China. AD - Guangdong Provincial Key Laboratory of Digestive Cancer Research, Shenzhen, China. FAU - Chen, Qiuxia AU - Chen Q AD - Department of Traditional Chinese Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. FAU - Wu, Jiaxin AU - Wu J AD - Digestive Medicine Center, the Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China. AD - Guangdong Provincial Key Laboratory of Digestive Cancer Research, Shenzhen, China. FAU - Hou, Haobin AU - Hou H AD - Digestive Medicine Center, the Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China. AD - Guangdong Provincial Key Laboratory of Digestive Cancer Research, Shenzhen, China. FAU - Chen, Huanjie AU - Chen H AD - Department of General Surgery, The First Affiliated Hospital of Shantou University Medical College, Shantou, China. FAU - Zhang, Weiliang AU - Zhang W AD - Department of Traditional Chinese Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. FAU - Zhu, Kaiyu AU - Zhu K AD - Center for Gastrointestinal Surgery, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. FAU - Liu, Baoyi AU - Liu B AD - Digestive Medicine Center, the Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China. AD - Guangdong Provincial Key Laboratory of Digestive Cancer Research, Shenzhen, China. FAU - Liang, Peiwen AU - Liang P AD - Digestive Medicine Center, the Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China. AD - Guangdong Provincial Key Laboratory of Digestive Cancer Research, Shenzhen, China. FAU - Zhang, Shijun AU - Zhang S AD - Department of Traditional Chinese Medicine, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China. FAU - Yang, Dongjie AU - Yang D AD - Digestive Medicine Center, the Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen, China. AD - Guangdong Provincial Key Laboratory of Digestive Cancer Research, Shenzhen, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Oncol JT - Frontiers in oncology JID - 101568867 PMC - PMC13714815 OTO - NOTNLM OT - bioinformatic OT - early-onset gastric cancer OT - hub genes OT - late-onset gastric cancer OT - machine learning COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 06:07 PHST- 2026/07/28 00:00 [received] PHST- 2026/09/06 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:07 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fonc.2026.1951255 [doi] PST - epublish SO - Front Oncol. 2026 Oct 14;16:1951255. doi: 10.3389/fonc.2026.1951255. eCollection 2026. PMID- 42953617 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2691-3704 (Electronic) IS - 2691-3704 (Linking) VI - 6 IP - 10 DP - 2026 Oct 26 TI - A Practical Palladium-Catalyzed Cysteine Arylation Platform for Late-Stage Functionalization of Peptides and Proteins. PG - 5868-5877 LID - 10.1021/jacsau.6c01159 [doi] AB - Site-selective modification of cysteine residues is central to the construction of peptide- and protein-based probes and therapeutics, yet existing methods are often constrained by linkage instability, narrow substrate scope, or harsh, nonbiocompatible reaction conditions. Here we report a palladium-catalyzed, site-selective cysteine arylation of peptides and proteins using readily accessible aryl thianthrenium salts. Enabled by the RuPhos-Pd-G3 catalyst, the reaction proceeds in aqueous buffer at 37 degrees C without added base, photoinitiator, or organic cosolvent, and tolerates a broad range of electron-rich, -neutral, and -deficient arylating reagents bearing diverse functional groups. Modification occurs with exclusive cysteine selectivity even amid numerous competing nucleophilic residues, enabling the installation of drug fragments, biotin, carbohydrates, fluorophores, and clickable handles onto unprotected peptides and the native protein BSA. As a methodological proof of concept, we further employ this strategy to assemble a receptor-targeting peptide-drug conjugate, establishing aryl thianthrenium salts as a versatile, biocompatible platform for stable aryl-thioether bioconjugation. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Guo, Quanping AU - Guo Q AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China. RINGGOLD: 12426 FAU - Wang, Chunlin AU - Wang C AD - School of Pharmacy, Lanzhou University, Lanzhou 730000, China. FAU - Ma, Xin AU - Ma X AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China. RINGGOLD: 12426 FAU - Mao, Wenbo AU - Mao W AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China. RINGGOLD: 12426 FAU - Liu, Pengyu AU - Liu P AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China. RINGGOLD: 12426 FAU - Chen, Kuan AU - Chen K AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China. RINGGOLD: 12426 FAU - Hu, Qingyu AU - Hu Q AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China. RINGGOLD: 12426 FAU - Wang, Mengran AU - Wang M AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China. RINGGOLD: 12426 FAU - Chen, Qiao AU - Chen Q AD - School of Pharmacy, Lanzhou University, Lanzhou 730000, China. FAU - Xu, Zhaoqing AU - Xu Z AUID- ORCID: 0000-0001-7663-6249 AD - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China. RINGGOLD: 12426 LA - eng PT - Journal Article DEP - 20261007 PL - United States TA - JACS Au JT - JACS Au JID - 101775714 PMC - PMC13714843 OTO - NOTNLM OT - aryl thianthrenium salts OT - bioconjugation OT - cysteine arylation OT - palladium catalysis OT - peptide-drug conjugates EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/07 CRDT- 2026/10/29 06:06 PHST- 2026/07/21 00:00 [received] PHST- 2026/09/11 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:06 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - 10.1021/jacsau.6c01159 [doi] PST - epublish SO - JACS Au. 2026 Oct 7;6(10):5868-5877. doi: 10.1021/jacsau.6c01159. eCollection 2026 Oct 26. PMID- 42953615 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2691-3704 (Electronic) IS - 2691-3704 (Linking) VI - 6 IP - 10 DP - 2026 Oct 26 TI - Direct Skeletal Modification of Bioactive N‑Fused Heterocycles via Transmutation. PG - 5798-5806 LID - 10.1021/jacsau.6c01052 [doi] AB - Advancements in pharmaceutical and agricultural lead discovery and optimization have been significantly enhanced through the precise editing of organic molecules at the atomic level, eliminating the need for prefunctionalization. Although many atom insertion and deletion reactions have been documented recently, instances of single-atom swaps are still limited, primarily due to the complexities involved in selectively cleaving and forming multiple chemical bonds surrounding the same atom. On the other hand, several natural products, dyes, and medications contain N-fused heterocycles that display a wide array of biological and photophysical activities. This situation presents an unexplored frontier for the skeleton editing of N-fused heterocycles. Herein, we disclose the straightforward single-atom editing of N-fused bioactive molecules by carbon-to-nitrogen transmutation. The reaction exhibits remarkable versatility and is capable of transforming a diverse array of functional groups containing heterocycles, including compounds with pharmaceutical relevance, thereby rendering it appropriate for a multitude of discovery initiatives. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Karan, Avijit AU - Karan A AD - Department of Chemistry, Visva-Bharati (A Central University), Santiniketan 731235, India. RINGGOLD: 30190 FAU - Neogi, Sukanya AU - Neogi S AD - Department of Chemistry, Visva-Bharati (A Central University), Santiniketan 731235, India. RINGGOLD: 30190 FAU - Hajra, Alakananda AU - Hajra A AUID- ORCID: 0000-0001-6141-0343 AD - Department of Chemistry, Visva-Bharati (A Central University), Santiniketan 731235, India. RINGGOLD: 30190 LA - eng PT - Journal Article DEP - 20260926 PL - United States TA - JACS Au JT - JACS Au JID - 101775714 PMC - PMC13714837 OTO - NOTNLM OT - N-fused heterocycles OT - Skeletal editing OT - bioactive molecules OT - carbon-to-nitrogen transmutation OT - modification of drug-like compounds EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/26 CRDT- 2026/10/29 06:06 PHST- 2026/07/06 00:00 [received] PHST- 2026/09/01 00:00 [revised] PHST- 2026/09/02 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:06 [entrez] PHST- 2026/09/26 00:00 [pmc-release] AID - 10.1021/jacsau.6c01052 [doi] PST - epublish SO - JACS Au. 2026 Sep 26;6(10):5798-5806. doi: 10.1021/jacsau.6c01052. eCollection 2026 Oct 26. PMID- 42953614 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1178-6973 (Print) IS - 1178-6973 (Electronic) IS - 1178-6973 (Linking) VI - 19 DP - 2026 TI - Metagenomic Next-Generation Sequencing Enhances Pathogen Detection and Prognostic Stratification in Hematopoietic Stem Cell Transplantation Recipients. PG - 617255 LID - 10.2147/IDR.S617255 [doi] LID - 617255 AB - BACKGROUND: As an emerging detection technique, metagenomic next-generation sequencing (mNGS) provides superior genomic resolution and enhances detection precision relative to conventional pathogen detection methods. Patients with malignant hematological disorders are susceptible to infections following hematopoietic stem cell transplantation (HSCT). mNGS demonstrates a significantly higher positivity rate compared with conventional testing. METHODS: A total of 100 patients who had undergone HSCT were retrospectively enrolled in this study. The detection rates and patient mortality rates associated with conventional diagnostic methods and mNGS were analyzed and compared. Additionally, the impact of mNGS results on the adjustment of antibacterial therapy was investigated, and the receiver operating characteristic (ROC) curve was utilized to assess the predictive value of mNGS for mortality. RESULTS: mNGS detected pathogens in 81% of patients (viruses 71.0%, with herpes simplex virus type 1 [HSV-1] at 26.7%; bacteria 25.0%, predominantly Enterobacteriaceae at 61.5%), outperforming conventional testing ( P < 0.001). The results indicated that C-reactive protein (CRP), age, length of hospital stay, transplantation modality, and granulocyte reconstitution time were independent predictors of mNGS-only positive results (P < 0.05). Antibiotic adjustment strategies aligned with mNGS findings were implemented in 59% of patients. The area under the curve (AUC) values for procalcitonin (PCT), CRP, granulocyte reconstitution time, megakaryocyte reconstitution time, and mNGS positivity alone in predicting mortality were 0.657, 0.756, 0.743, 0.724, and 0.539, respectively. The combined AUC of these five indicators was 0.812 (95% CI: 0.694-0.929). CONCLUSION: Compared with conventional methods, mNGS has a broader pathogen detection spectrum, which may improve the overall detection rate of unknown, uncommon, and mixed infectious pathogens. The selection of diagnostic modalities may be guided by patient-specific factors including CRP levels, age, length of hospital stay, transplantation modality, and granulocyte reconstitution time. mNGS informs antibiotic adjustment, while combined biomarkers (AUC = 0.812) provide prognostic value. CI - (c) 2026 Liu et al. FAU - Liu, Ying AU - Liu Y AUID- ORCID: 0009-0001-6758-3816 AD - Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China. FAU - Bian, Xintong AU - Bian X AD - Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China. FAU - Wang, Hongxu AU - Wang H AUID- ORCID: 0000-0002-2193-8458 AD - Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China. FAU - Tang, Dijiao AU - Tang D AD - Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, People's Republic of China. LA - eng PT - Journal Article DEP - 20261023 PL - New Zealand TA - Infect Drug Resist JT - Infection and drug resistance JID - 101550216 PMC - PMC13714704 OTO - NOTNLM OT - HSCT OT - detection rate OT - mNGS OT - survival analysis COIS- The authors declare that they have no competing interests. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/23 CRDT- 2026/10/29 06:06 PHST- 2026/04/15 00:00 [received] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:06 [entrez] PHST- 2026/10/23 00:00 [pmc-release] AID - 617255 [pii] AID - 10.2147/IDR.S617255 [doi] PST - epublish SO - Infect Drug Resist. 2026 Oct 23;19:617255. doi: 10.2147/IDR.S617255. eCollection 2026. PMID- 42953613 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2691-3704 (Electronic) IS - 2691-3704 (Linking) VI - 6 IP - 10 DP - 2026 Oct 26 TI - High-throughput Materials-to-Device Discovery of Lead-free Double Perovskites Beyond Radiative-limit Metrics. PG - 5722-5733 LID - 10.1021/jacsau.6c00980 [doi] AB - Metal halide perovskite solar cells (PSCs) are leading candidates for next-generation photovoltaics, yet their instability and lead toxicity motivate environmentally benign alternatives. Lead-free double perovskites offer improved structural robustness but often exhibit weak near-band-edge absorption and modest efficiencies, making it crucial to identify compositions and phases that balance stability with photovoltaic performance. Here we assemble an initial enumerated phase-diverse library of 31,276 structure-composition entries spanning six symmetry-distinct cubic, tetragonal, and monoclinic configurations and establish a device-guided multiscale workflow for realistic performance evaluation. This framework combines symmetry-aware optical screening, finite-temperature structural-retention assessment, multiscale parameter transfer, 300 K thermal-displacement-averaged optical response, and self-consistent drift-diffusion device modeling. The workflow selects 23 candidates for comparative device-level evaluation under unified conditions. The device-level analysis shows that candidates favored by bandgap or Spectroscopic Limited Maximum Efficiency (SLME) do not necessarily retain their advantage as recombination losses increase. By varying the effective bimolecular recombination coefficient and the Shockley-Read-Hall carrier lifetime, we quantify PCE degradation and ranking evolution as a model-conditioned measure of device-level loss tolerance. The shortlisted candidates reach baseline predicted PCEs of up to 15.26%, while pronounced rank changes under increasing nonradiative recombination loss highlight the limitations of radiative-limit metrics alone. This work therefore recasts lead-free double-perovskite screening from identifying the highest radiative-limit efficiency to prioritizing materials that combine competitive device performance with robust loss tolerance and experimental relevance. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Wang, Yuanxin AU - Wang Y AD - State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, P. R. China. AD - University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Meng, Lingyi AU - Meng L AUID- ORCID: 0000-0002-8985-5093 AD - State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, P. R. China. AD - Xiamen Key Laboratory of Rare Earth Photoelectric Functional Materials, Xiamen Institute of Rare Earth Materials, Haixi Institutes, Chinese Academy of Sciences, Xiamen 361021, P. R. China. AD - University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Zhuang, Wei AU - Zhuang W AUID- ORCID: 0000-0003-4106-0985 AD - State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, P. R. China. AD - University of Chinese Academy of Sciences, Beijing 100049, China. LA - eng PT - Journal Article DEP - 20261007 PL - United States TA - JACS Au JT - JACS Au JID - 101775714 PMC - PMC13714825 OTO - NOTNLM OT - drift-diffusion device simulation OT - first-principles calculations OT - high-throughput screening OT - lead-free double perovskites OT - multiscale modeling OT - nonradiative recombination EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/07 CRDT- 2026/10/29 06:06 PHST- 2026/06/22 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:06 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - 10.1021/jacsau.6c00980 [doi] PST - epublish SO - JACS Au. 2026 Oct 7;6(10):5722-5733. doi: 10.1021/jacsau.6c00980. eCollection 2026 Oct 26. PMID- 42953608 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-1078 (Print) IS - 1664-1078 (Electronic) IS - 1664-1078 (Linking) VI - 17 DP - 2026 TI - Operationalizing the physiological state (P) in nonlinear behavioral systems: a measurement framework for state-dependent dynamics. PG - 1873356 LID - 10.3389/fpsyg.2026.1873356 [doi] LID - 1873356 AB - Behavioral outcomes may vary within the same individual even when measured environmental conditions appear comparable. To test whether an organism's changing physiological condition provides information about such variation, this article proposes an operational measurement framework for Physiological State (P). P is defined as a latent, multidimensional, and time-dependent configuration generated by ongoing Physiological Organization. Antecedent conditions (A) and consequence-related conditions (C) remain external environmental variables, whereas behavior (B) is modeled as a separate downstream outcome. Rather than uniquely determining behavior, P influences Physiological Accessibility-the state-dependent availability of existing Adaptive Capacities for recruitment and use-and thereby constrains behavioral expression. The construct is distinguished from emotional and cognitive constructs, subjective reports, observable behavior, stress, allostatic load, and individual physiological indicators. Operationalizing requires prespecifying the target behavior and timescale; selecting reliable, sensitive, feasible, and nonredundant physiological indicators; aligning and preprocessing observations; deriving dynamic features; integrating those features through an identifiable latent-state model; representing estimated uncertainty; and conducting prospective validation. The Thirteen Physiological Systems and Seven Layers of Physiological State guide physiological coverage and interpretation but do not correspond one-to-one with the latent dimensions. In a candidate nonlinear state-space implementation, physiological observations, Y(t), provide evidence for estimating P(t), while state evolution depends on preceding P, current A, and relevant prior C. Filtered estimates use only information available through the prediction time. Because B remains outside the physiological observation vector and is evaluated prospectively as B(t + Delta), the framework avoids circularly defining the state by the outcome it is intended to predict. Empirical support would require reliable, interpretable, and temporally coherent state estimates that demonstrate reproducible prospective and incremental value beyond environmental variables, prior behavior, isolated physiological indicators, established single constructs, simple physiological composites, and appropriate linear or lower-dimensional alternatives. Failure to obtain identifiable state estimates or incremental prospective information about B would count against the proposed operationalization. The framework is theoretically and methodologically developmental and should not be interpreted as a validated instrument, diagnostic measure, or universal physiological score. CI - Copyright (c) 2026 Gomez Uncu. FAU - Gomez Uncu, Yoandra M AU - Gomez Uncu YM AD - Beautiful Minds ABA Services, Fort Myers, FL, United States. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Psychol JT - Frontiers in psychology JID - 101550902 PMC - PMC13714711 OTO - NOTNLM OT - latent-state estimation OT - nonlinear state-space modeling OT - nonlinear systems OT - physiological accessibility OT - physiological state OT - psychophysiological measurement OT - state-dependent behavior COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 06:06 PHST- 2026/05/05 00:00 [received] PHST- 2026/09/10 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:06 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpsyg.2026.1873356 [doi] PST - epublish SO - Front Psychol. 2026 Oct 14;17:1873356. doi: 10.3389/fpsyg.2026.1873356. eCollection 2026. PMID- 42953599 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2379-6146 (Electronic) IS - 2379-6146 (Linking) VI - 10 IP - 4 DP - 2026 Oct TI - Measuring Maturity: Development and Validation of the Learning Health System Self-Assessment Tool. PG - e70123 LID - 10.1002/lrh2.70123 [doi] LID - e70123 AB - BACKGROUND: The Learning Health System (LHS) concept holds significant promise for continuous, data-driven improvement in healthcare, yet operationalization at scale remains elusive. LHS maturity can be conceptualized as the extent to which organizations demonstrate essential capabilities. Current tools to assess LHS maturity have limited scope, and a rapid, multi-stakeholder tool to assess organizational-level LHS maturity remains lacking. METHODS: To address this gap, we developed a maturity self-assessment tool mapped to the LHS Action Framework by Reid and colleagues that operationalizes LHS maturity as capability levels across five core learning activities and selected infrastructure supports. Using design science and participatory action research, we conducted five sequential development steps: (1) item bank creation, (2) prioritization and refinement by the core research team, (3) expert panel review, (4) cognitive interviews with interprofessional decision-makers, and (5) pilot testing in a real-world setting. Each step incorporated participant feedback to refine and streamline the tool. RESULTS: The resulting tool consists of 41 items assessing the extent to which LHS activities occur across the LHS Action Framework's five learning activities and supporting infrastructure. Preliminary findings from a national Emergency Department LHS assessment (n = 53 responses from 33 sites) demonstrated the tool's feasibility and acceptability in a real-world setting, and its ability to identify capability gaps across the LHS Action Framework's constructs. CONCLUSIONS: The self-assessment tool provides a foundational measurement approach to guide LHS development, representing a critical step toward developing a comprehensive LHS maturity model. By facilitating a shared understanding of the LHS and enabling self-assessment of the current-state capabilities as core LHS maturity indicators, the tool may support evidence-informed and collaborative strategic planning for a variety of health system configurations. Future research should evaluate the tool's generalizability across diverse contexts and utility for tracking developmental progress over time. CI - (c) 2026 The Author(s). Learning Health Systems published by Wiley Periodicals LLC on behalf of University of Michigan. FAU - Rentes, Victor C AU - Rentes VC AUID- ORCID: 0000-0001-9214-3815 AD - Institute of Health Policy, Management and Evaluation University of Toronto Toronto Ontario Canada. AD - Institute for Better Health Trillium Health Partners Mississauga Ontario Canada. FAU - Reid, Robert J AU - Reid RJ AUID- ORCID: 0000-0002-9008-6375 AD - Institute of Health Policy, Management and Evaluation University of Toronto Toronto Ontario Canada. AD - Institute for Better Health Trillium Health Partners Mississauga Ontario Canada. AD - Temerty Faculty of Medicine, University of Toronto Toronto Ontario Canada. FAU - Greene, Sarah M AU - Greene SM AUID- ORCID: 0009-0004-4531-6575 AD - National Academy of Medicine Washington DC USA. FAU - Cheng, Ivy AU - Cheng I AD - Sunnybrook Health Sciences Centre Toronto Ontario Canada. AD - Division of Emergency Medicine University of Toronto Toronto Ontario Canada. FAU - Wodchis, Walter P AU - Wodchis WP AUID- ORCID: 0000-0003-2494-7031 AD - Institute of Health Policy, Management and Evaluation University of Toronto Toronto Ontario Canada. AD - Institute for Better Health Trillium Health Partners Mississauga Ontario Canada. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Learn Health Syst JT - Learning health systems JID - 101708071 PMC - PMC13716532 OTO - NOTNLM OT - health system transformation OT - implementation science OT - learning health systems OT - maturity model OT - organizational capability OT - self-assessment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/28 CRDT- 2026/10/29 06:05 PHST- 2026/04/23 00:00 [received] PHST- 2026/08/03 00:00 [revised] PHST- 2026/08/27 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:05 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - LRH270123 [pii] AID - 10.1002/lrh2.70123 [doi] PST - epublish SO - Learn Health Syst. 2026 Oct 28;10(4):e70123. doi: 10.1002/lrh2.70123. eCollection 2026 Oct. PMID- 42953598 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2691-3704 (Electronic) IS - 2691-3704 (Linking) VI - 6 IP - 10 DP - 2026 Oct 26 TI - Machine Learning-Guided Strategy Selection and Condition Prediction for Photoredox Catalysis. PG - 5595-5604 LID - 10.1021/jacsau.6c00471 [doi] AB - Photoredox catalysis offers powerful opportunities for organic synthesis, yet its broader application remains limited by the difficulty of identifying viable synthetic routines and conditions. Here, we present an experimentally validated, machine-learning-assisted workflow that combines literature-precedent retrieval with reaction-condition recommendation to support chemist-guided design of photoredox transformations. With 11,069 curated literature-derived reaction records, a variational autoencoder organizes photoredox reactions in a learned latent space to enable reaction-space navigation and retrieval of traceable synthetic precedents, while random-forest classifiers recommend feasible catalyst and reagent classes for proposed reactant-product pairs. External validation on unseen literature examples and experimental validation of selected model-guided proposals demonstrates the practical utility of the workflow. It further enabled three unreported photoredox transformations, highlighting its possibility for generating experimentally testable synthetic hypotheses. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Ye, Xiaoyu AU - Ye X AD - The Institute for Advanced Studies (IAS), Wuhan University, Wuhan 430072, China. RINGGOLD: 12390 FAU - Xu, Jiaxin AU - Xu J AD - The Institute for Advanced Studies (IAS), Wuhan University, Wuhan 430072, China. RINGGOLD: 12390 FAU - Tian, Xiaohui AU - Tian X AD - HOURS Inc., Suzhou 215000, China. FAU - Fan, Dongyuan AU - Fan D AD - The Institute for Advanced Studies (IAS), Wuhan University, Wuhan 430072, China. RINGGOLD: 12390 FAU - Ran, Jingdi AU - Ran J AD - The Institute for Advanced Studies (IAS), Wuhan University, Wuhan 430072, China. RINGGOLD: 12390 FAU - Zeng, Xiaodong AU - Zeng X AD - Shandong Laboratory of Yantai Drug Discovery, Bohai Rim Advanced Research Institute for Drug Discovery, Yantai 264117, China. FAU - Cai, Yunqi AU - Cai Y AD - Beijing National Laboratory for Condensed Matter Physics and Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China. FAU - Seeberger, Peter H AU - Seeberger PH AUID- ORCID: 0000-0003-3394-8466 AD - Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Potsdam 14476, Germany. RINGGOLD: 28321 FAU - Zeng, Zuo AU - Zeng Z AD - HOURS Inc., Suzhou 215000, China. FAU - Wang, Xiang Simon AU - Wang XS AD - Howard University College of Pharmacy, 2300 Fourth Street NW, Washington, District of Columbia 20059, United States. FAU - Xiao, Yuling AU - Xiao Y AD - State Key Laboratory of Drug Research & Center of Pharmaceutics, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. AD - Shandong Laboratory of Yantai Drug Discovery, Bohai Rim Advanced Research Institute for Drug Discovery, Yantai 264117, China. FAU - Chen, Yi-Hung AU - Chen YH AUID- ORCID: 0000-0001-6731-1119 AD - The Institute for Advanced Studies (IAS), Wuhan University, Wuhan 430072, China. RINGGOLD: 12390 LA - eng PT - Journal Article DEP - 20260924 PL - United States TA - JACS Au JT - JACS Au JID - 101775714 PMC - PMC13714848 OTO - NOTNLM OT - autoencoder OT - machine-learning-assisted workflow OT - organic synthesis OT - photoredox transformations OT - random-forest classifiers EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/24 CRDT- 2026/10/29 06:05 PHST- 2026/03/27 00:00 [received] PHST- 2026/07/28 00:00 [revised] PHST- 2026/07/29 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:05 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - 10.1021/jacsau.6c00471 [doi] PST - epublish SO - JACS Au. 2026 Sep 24;6(10):5595-5604. doi: 10.1021/jacsau.6c00471. eCollection 2026 Oct 26. PMID- 42953597 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1662-0631 (Print) IS - 1662-0631 (Electronic) IS - 1662-0631 (Linking) VI - 20 IP - 1 DP - 2026 Jan-Dec TI - Ultra-Short-Interval Staged Pancreatoduodenectomy after Minimally Invasive Direct Coronary Artery Bypass for Ampullary Carcinoma in a Patient with Severe Coronary Artery Disease: A Case Report. PG - 474-481 LID - 10.1159/crg/abwag017 [doi] AB - INTRODUCTION: Managing patients with concurrent ampullary carcinoma and severe coronary artery disease (CAD) poses a formidable surgical dilemma. Pancreatoduodenectomy (PD) is a highly invasive procedure with significant bleeding risks, while symptomatic CAD requires urgent revascularization. Standard percutaneous coronary intervention necessitates dual antiplatelet therapy (DAPT), which increases perioperative hemorrhagic complications. However, delaying PD to complete cardiac treatment risks tumor progression. We present a strategic "ultra-short-interval" staged approach to balance oncological urgency with cardiac safety. CASE PRESENTATION: A man in his 70s presented with severe anemia (Hb 6.6 g/dL) and exertional dyspnea (CCS class III/IV). Investigations revealed stage IB ampullary adenocarcinoma and severe CAD, including 90% stenosis in the left anterior descending artery. To avoid the bleeding risks of DAPT and the systemic trauma of conventional coronary artery bypass grafting, minimally invasive direct coronary artery bypass (MIDCAB) was performed. An ultra-short-interval staged approach was strategically planned, with PD scheduled just 7 days after MIDCAB. Despite transient postoperative hematochezia after the first procedure, the patient remained stable. As planned, subtotal stomach-preserving PD was successfully performed 1 week later without cardiovascular events. The patient was discharged on postoperative day 31. CONCLUSION: This ultra-short-interval staged approach - MIDCAB followed by PD within seven days - is a potent strategy for high-risk candidates. It facilitates essential coronary revascularization without DAPT or cardiopulmonary bypass, thereby minimizing oncological delay and tumor progression risks while mitigating the risk of catastrophic perioperative myocardial infarction or hemorrhage. CI - (c) The Author(s) 2026. Published by S. Karger AG. FAU - Yamamoto, Tenchi AU - Yamamoto T AD - Department of Surgery, Saitama Medical Center, Jichi Medical University, Saitama, Japan. FAU - Watanabe, Fumiaki AU - Watanabe F AD - Department of Surgery, Saitama Medical Center, Jichi Medical University, Saitama, Japan. FAU - Mizusawa, Yuki AU - Mizusawa Y AD - Department of Surgery, Saitama Medical Center, Jichi Medical University, Saitama, Japan. FAU - Aizawa, Hidetoshi AU - Aizawa H AD - Department of Surgery, Saitama Medical Center, Jichi Medical University, Saitama, Japan. FAU - Endo, Yuhei AU - Endo Y AD - Department of Surgery, Saitama Medical Center, Jichi Medical University, Saitama, Japan. FAU - Noda, Hiroshi AU - Noda H AD - Department of Surgery, Saitama Medical Center, Jichi Medical University, Saitama, Japan. FAU - Rikiyama, Toshiki AU - Rikiyama T AD - Department of Surgery, Saitama Medical Center, Jichi Medical University, Saitama, Japan. LA - eng PT - Case Reports PT - Journal Article DEP - 20261007 PL - Switzerland TA - Case Rep Gastroenterol JT - Case reports in gastroenterology JID - 101474819 PMC - PMC13716405 OTO - NOTNLM OT - Ampullary carcinoma OT - Coronary artery disease OT - Minimally invasive direct coronary artery bypass OT - Pancreatoduodenectomy COIS- The authors have no conflicts of interest to declare. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/07 CRDT- 2026/10/29 06:05 PHST- 2026/02/12 00:00 [received] PHST- 2026/09/01 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:05 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - 2026-2-8 [pii] AID - 10.1159/crg/abwag017 [doi] PST - epublish SO - Case Rep Gastroenterol. 2026 Oct 7;20(1):474-481. doi: 10.1159/crg/abwag017. eCollection 2026 Jan-Dec. PMID- 42953592 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2673-5059 (Electronic) IS - 2673-5059 (Linking) VI - 7 DP - 2026 TI - Dietary supplements are commonly used for menopause symptoms amid concerns about hormone therapy: a cross-sectional study. PG - 1924461 LID - 10.3389/fgwh.2026.1924461 [doi] LID - 1924461 AB - OBJECTIVE: Many women use dietary supplements to manage menopause symptoms, yet patterns of use, perceived effectiveness, and information sources remain incompletely described in contemporary literature. This cross-sectional study assessed current dietary supplement use, including use specifically for menopause symptoms, and perceptions of menopausal hormone therapy (MHT) among symptomatic menopausal women aged 45-65 years. METHODS: An online questionnaire was administered to a self-selected sample of 226 perimenopausal or postmenopausal participants recruited through online health forums, social media, menopause support groups, and newsletters. Survey items captured sociodemographic characteristics, health, supplement use, perceived effectiveness, information sources, and MHT awareness and concerns. Associations were examined using cross-tabulations, chi-square and exact tests, Spearman correlations, and logistic regression. RESULTS: Current use of at least one dietary supplement, for any purpose, was reported by 138 participants (61.1%; 95% CI 54.6-67.2). Self-reported use of supplements specifically to manage menopause symptoms (menopause-targeted use) was reported by 65 participants (28.9%; 95% CI 23.4-35.1). Among supplement users, the most frequently reported products were multivitamins (55.8%), vitamin D (52.9%), magnesium (51.4%), and probiotics (41.3%). MHT awareness was high (n = 187, 82.7%) and increased with income. Concerns about breast cancer (47.8%), blood clotting (44.7%), stroke (40.7%), and heart disease (37.2%) as risks of MHT were frequently reported. Healthcare providers and online sources were the leading sources of information, and social media influencers were rated least trustworthy. Former smokers (adjusted OR 2.98, 95% CI 1.21-7.34) and never smokers (adjusted OR 3.92, 95% CI 1.59-9.65) had higher odds of any supplement use than current smokers; this association was not observed for menopause-targeted use. CONCLUSION: In this self-selected online sample, current dietary supplement use for any purpose was common, although fewer than half of supplement users reported using supplements to manage menopause symptoms. Concerns about MHT persisted despite high awareness. These estimates should not be generalized to the broader population of midlife women. Future studies using probability-based samples and validated symptom measures are needed to characterize menopause-targeted supplement use and to inform clinical strategies that support individualized menopause care. CI - (c) 2026 Mitschke. FAU - Mitschke, Susanne AU - Mitschke S AD - Harvard T.H. Chan School of Public Health, Boston, MA, United States. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Glob Womens Health JT - Frontiers in global women's health JID - 101776281 PMC - PMC13714707 OTO - NOTNLM OT - dietary supplements OT - menopausal hormone therapy OT - menopause OT - menopause symptom management OT - women's health COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/13 CRDT- 2026/10/29 06:05 PHST- 2026/06/30 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:05 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fgwh.2026.1924461 [doi] PST - epublish SO - Front Glob Womens Health. 2026 Oct 13;7:1924461. doi: 10.3389/fgwh.2026.1924461. eCollection 2026. PMID- 42953588 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1177-8881 (Electronic) IS - 1177-8881 (Linking) VI - 20 DP - 2026 TI - TREM2 Signaling and Alzheimer's Disease: Experimental Modulators, Progress, and Challenges. PG - 611558 LID - 10.2147/DDDT.S611558 [doi] LID - 611558 AB - Alzheimer's Disease (AD) is the leading cause of dementia worldwide, with a growing global burden and limited disease-modifying therapeutic options. The transmembrane receptor termed triggering receptor expressed on myeloid cells 2 (TREM2), which is mainly expressed by myeloid cells, has emerged as a target of interest in AD due to its role in modulating microglia functions, including cholesterol metabolism, phagocytosis of amyloid-beta (Abeta), inflammatory signaling, and innate immune neuronal protection. Genetic research has also shown that partial loss-of-function mutations in the TREM2 gene, particularly the R47H and R62H variants, substantially increase the risk of late-onset AD, further underscoring the therapeutic rationale for enhancing TREM2 signaling to combat the disease. Therapeutic strategies targeting TREM2 have exponentially increased in recent years, spanning monoclonal antibodies (mAbs) with distinct epitopes and mechanisms of receptor modulation, orally bioavailable small-molecule drugs capable of penetrating the blood-brain barrier (BBB), and upstream mechanisms targeting the shedding of the TREM2 ectodomain. Early clinical trials have revealed that target engagement does not translate directly into clinical benefit in patients, highlighting knowledge gaps in the timing of intervention, patient selection, dose optimization, and the complex dichotomy of TREM2 signaling, in which both insufficient and excessive activation may be detrimental to AD patients. Nonetheless, the continued advancement of therapeutics into clinical trials reflects the confidence in the underlying scientific rationale. This narrative review summarizes the current understanding of wild-type and variant TREM2 signaling in AD pathogenesis, evaluates the preclinical and clinical evidence supporting each class of TREM2-targeted therapeutic strategies, and discusses the challenges, emerging insights, and future directions that could shape the next generation of TREM2-based interventions for AD. CI - (c) 2026 Sayegh et al. FAU - Sayegh, Mark AU - Sayegh M AD - School of Medicine, Creighton University, Phoenix, AZ, USA. FAU - Azarfar, Katia AU - Azarfar K AUID- ORCID: 0009-0006-1797-7178 AD - Department of Pharmacology and Neuroscience, Texas Tech University Health Sciences Center, Lubbock, TX, USA. FAU - Reyna, Ellie Marie AU - Reyna EM AUID- ORCID: 0009-0002-6804-1995 AD - Department of Pharmacology and Neuroscience, Texas Tech University Health Sciences Center, Lubbock, TX, USA. FAU - Kennedy, Jessica Marianne AU - Kennedy JM AUID- ORCID: 0009-0007-6230-9862 AD - Department of Pharmacology and Neuroscience, Texas Tech University Health Sciences Center, Lubbock, TX, USA. FAU - Sabbagh, Marwan N AU - Sabbagh MN AD - School of Medicine, Creighton University, Phoenix, AZ, USA. AD - Department of Neurology, Barrow Neurological Institute, Phoenix, AZ, USA. FAU - Decourt, Boris AU - Decourt B AUID- ORCID: 0000-0001-7637-5034 AD - Department of Pharmacology and Neuroscience, Texas Tech University Health Sciences Center, Lubbock, TX, USA. LA - eng PT - Journal Article PT - Review DEP - 20261023 PL - New Zealand TA - Drug Des Devel Ther JT - Drug design, development and therapy JID - 101475745 RN - 0 (Receptors, Immunologic) RN - 0 (Membrane Glycoproteins) RN - 0 (TREM2 protein, human) SB - IM MH - Humans MH - *Alzheimer Disease/drug therapy/metabolism MH - *Receptors, Immunologic/metabolism/genetics MH - *Membrane Glycoproteins/metabolism/genetics/antagonists & inhibitors MH - *Signal Transduction/drug effects MH - Animals PMC - PMC13714702 OTO - NOTNLM OT - Alzheimer's disease OT - TREM2 OT - microglia OT - myeloid cells OT - neuroinflammation COIS- Sabbagh is an Editor-in-Chief of Neurology and Therapy. Dr. Sabbagh has served on the Board of Directors for EIP Pharma/CervoMed and consulted for Roche-Genentech, Eisai, Lilly, NeuroTherapia, Novo Nordisk, Anavex, Cognito Therapeutics, GSK, Abbvie, Lighthouse Pharmaceuticals, Alzheon, and Athira. Dr. Decourt consults for Lighthouse Pharmaceuticals. Dr. Decourt also reports grants or contracts from Alzheimer's Association outside the submitted work. The authors report no other conflicts of interest in this work. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/23 CRDT- 2026/10/29 06:04 PHST- 2026/06/16 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:04 [entrez] PHST- 2026/10/23 00:00 [pmc-release] AID - 611558 [pii] AID - 10.2147/DDDT.S611558 [doi] PST - epublish SO - Drug Des Devel Ther. 2026 Oct 23;20:611558. doi: 10.2147/DDDT.S611558. eCollection 2026. PMID- 42953581 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2035-8164 (Electronic) IS - 2035-8237 (Print) IS - 2035-8164 (Linking) VI - 18 DP - 2026 TI - Fluoroscopy-Guided Intra-Articular Hip Corticosteroid Injections: Diagnostic and Therapeutic Utility, Technique, and Steroid Selection. PG - 172698 LID - 10.52965/001c.172698 [doi] LID - 172698 AB - Intra-articular hip corticosteroid injection is a practical option for selected patients with hip pain, particularly osteoarthritis, and may also clarify whether symptoms arise from the hip rather than the lumbar spine or periarticular structures. This review evaluates the diagnostic and therapeutic utility of injections performed under fluoroscopy, summarizes technique, and discusses steroid selection and dosing. # Recent Findings Randomized trials and contemporary syntheses support clinically meaningful short-term improvement in pain and function after image-guided hip corticosteroid injection, although durability is variable and repeated-injection data remain limited. Fluoroscopy provides continuous visualization of osseous landmarks and contrast-confirmed intra-articular spread. Ultrasound avoids radiation and may be more convenient depending on physician comfort level and ultrasound skills. Safety discussions have emphasized transient systemic effects, uncertain associations with rapidly progressive osteoarthritis, and the timing of injection before arthroplasty. # Summary Fluoroscopy-guided injection remains a reliable, reproducible diagnostic and therapeutic tool when the pain generator is uncertain or short-term symptom control may facilitate rehabilitation, bridge care, or support shared surgical decision-making. Benefit depends on careful patient selection, technically confirmed intra-articular delivery, conservative dosing, and explicit counseling that relief is usually temporary and that structural disease is not reversed. FAU - Hasoon, Jamal AU - Hasoon J AD - Department of Anesthesiology, Critical Care, and Pain Medicine The University of Texas Health Science Center at Houston. ROR: https://ror.org/03gds6c39 FAU - Urits, Ivan AU - Urits I AD - Department of Anesthesiology, Critical Care, and Pain Medicine The University of Texas Health Science Center at Houston. ROR: https://ror.org/03gds6c39 FAU - Imani, Farnad AU - Imani F AD - Department of Anesthesiology, Critical Care, and Pain Medicine The University of Texas Health Science Center at Houston. ROR: https://ror.org/03gds6c39 FAU - Varrassi, Giustino AU - Varrassi G AD - Department of Anesthesiology, Critical Care, and Pain Medicine The University of Texas Health Science Center at Houston. ROR: https://ror.org/03gds6c39 FAU - Viswanath, Omar AU - Viswanath O AD - Department of Anesthesiology, Critical Care, and Pain Medicine The University of Texas Health Science Center at Houston. ROR: https://ror.org/03gds6c39 LA - eng PT - Journal Article DEP - 20261026 PL - United States TA - Orthop Rev (Pavia) JT - Orthopedic reviews JID - 101524779 PMC - PMC13716511 OTO - NOTNLM OT - corticosteroid OT - diagnostic injection OT - fluoroscopy OT - hip osteoarthritis OT - interventional pain OT - intra-articular injection EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/26 CRDT- 2026/10/29 06:04 PHST- 2026/09/06 00:00 [received] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:04 [entrez] PHST- 2026/10/26 00:00 [pmc-release] AID - 172698 [pii] AID - 10.52965/001c.172698 [doi] PST - epublish SO - Orthop Rev (Pavia). 2026 Oct 26;18:172698. doi: 10.52965/001c.172698. eCollection 2026. PMID- 42953579 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2198-7793 (Print) IS - 2198-7793 (Electronic) IS - 2198-7793 (Linking) VI - 12 IP - 1 DP - 2026 TI - Malignant Perivascular Epithelioid Cell Tumor Originating from the Transverse Mesocolon. LID - 10.70352/scrj.cr.26-0592 [doi] LID - 26-0592 AB - INTRODUCTION: Perivascular epithelioid cell tumors (PEComas) are rare mesenchymal neoplasms with melanocytic and smooth muscle differentiation. Primary mesenteric PEComas are exceptionally uncommon and usually cannot be diagnosed from imaging alone. We report a case of malignant PEComa arising from the transverse mesocolon. CASE PRESENTATION: A 34-year-old woman was referred to our department after abdominal ultrasonography showed a large intra-abdominal mass. Contrast-enhanced CT revealed a 15-cm heterogeneous, hypervascular mesenteric tumor with internal necrosis and feeding vessels from the superior mesenteric artery. MRI showed no continuity with the uterus or ovaries. Because percutaneous biopsy was considered to carry a substantial risk of bleeding and dissemination, the tumor was resected for diagnosis and treatment. Laparotomy demonstrated a transverse-mesocolic tumor directly invading the transverse colon; transverse colectomy with en bloc tumor resection was performed. Histopathological evaluation showed pleomorphic epithelioid cells, marked nuclear atypia, coagulative necrosis, vascular invasion, and frequent mitoses. The tumor was positive for HMB-45, Melan-A, and smooth muscle actin and negative for TFE3, supporting malignant PEComa. The postoperative course was uneventful, and the patient remained free of recurrence 4 months after surgery. CONCLUSIONS: Malignant PEComa of the transverse mesocolon is extremely rare. This tumor should be considered among large, hypervascular mesenteric tumors, although imaging is not specific. Complete resection with negative margins is the principal treatment for localized disease. High-risk pathological features justify prolonged cross-sectional imaging surveillance and multidisciplinary, molecularly informed management if recurrence develops. CI - (c) 2026 The Author(s). Published by Japan Surgical Society. FAU - Yokota, Ryo AU - Yokota R AD - Department of Gastroenterological Surgery, Nippon Medical School Musashikosugi Hospital, Kawasaki, Kanagawa, Japan. FAU - Ohta, Ryo AU - Ohta R AD - Department of Gastroenterological Surgery, Nippon Medical School Musashikosugi Hospital, Kawasaki, Kanagawa, Japan. FAU - Takeda, Kohki AU - Takeda K AD - Department of Gastroenterological Surgery, Nippon Medical School Musashikosugi Hospital, Kawasaki, Kanagawa, Japan. FAU - Sekiguchi, Kumiko AU - Sekiguchi K AD - Department of Gastroenterological Surgery, Nippon Medical School Musashikosugi Hospital, Kawasaki, Kanagawa, Japan. FAU - Shimizu, Takao AU - Shimizu T AD - Department of Gastroenterological Surgery, Nippon Medical School Musashikosugi Hospital, Kawasaki, Kanagawa, Japan. FAU - Motoda, Norio AU - Motoda N AD - Department of Diagnostic Pathology, Nippon Medical School Musashikosugi Hospital, Kawasaki, Kanagawa, Japan. FAU - Taniai, Nobuhiko AU - Taniai N AD - Department of Gastroenterological Surgery, Nippon Medical School Musashikosugi Hospital, Kawasaki, Kanagawa, Japan. FAU - Matsuda, Akihisa AU - Matsuda A AD - Department of Gastroenterological Surgery, Nippon Medical School, Tokyo, Japan. LA - eng PT - Case Reports PT - Journal Article DEP - 20261028 PL - Japan TA - Surg Case Rep JT - Surgical case reports JID - 101662125 PMC - PMC13716536 OTO - NOTNLM OT - malignant perivascular epithelioid cell tumors OT - mesentery OT - perivascular epithelioid cell tumors (PEComas) OT - surgical resection OT - transverse mesocolon COIS- The authors declare that they have no competing interests. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/28 CRDT- 2026/10/29 06:04 PHST- 2026/07/27 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 06:04 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - scrj.cr.26-0592 [pii] AID - 10.70352/scrj.cr.26-0592 [doi] PST - ppublish SO - Surg Case Rep. 2026;12(1):26-0592. doi: 10.70352/scrj.cr.26-0592. Epub 2026 Oct 28. PMID- 42953572 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2813-2424 (Electronic) IS - 2813-2424 (Linking) VI - 5 DP - 2026 TI - The Pampa del Indio Project: long-term variations of Trypanosoma cruzi infection in Triatoma sordida, a non-target invasive vector. PG - 1953889 LID - 10.3389/fpara.2026.1953889 [doi] LID - 1953889 AB - INTRODUCTION: The Gran Chaco region still faces vector-borne transmission of Trypanosoma cruzi by the main vector, Triatoma infestans. In Pampa del Indio, a municipality belonging to the Argentinian Chaco, a long-term research and intervention program achieved the quasielimination of T. infestans from rural houses and halted the domestic vector-borne transmission of T. cruzi. Here, we evaluate the occurrence and distribution of T. cruzi infection in Triatoma sordida sensu lato, a secondary species that frequently invades rural domiciles and colonizes the peridomestic environment, over 2007-2016. Based on the prevalence of infection reported across the Gran Chaco region in the absence of T. infestans and given the limited sensitivity of optical microscopy, we hypothesized that T. cruzi infection in T. sordida post-intervention would be higher than in our previous reports (0-6%). METHODS AND RESULTS: Of 10,728 T. sordida collected at 528 premises along the intervention, we examined 1,946 specimens by OM or vkDNA-PCR and found a naive prevalence of T. cruzi infection of 1.44% (95% CI: 0.10% -2.07%). The mean number of infected T. sordida per house was 1.12 (SD = 0.33). Infection was higher in adults rather than nymphs; maximal in domiciles, intermediate in peridomestic kitchens, storerooms and granaries, and minimal in other peridomestic sites. Spatial analysis identified an infection hotspot in the northwestern section of the municipality. By means of single-season occupancy models, we estimated that the detection probability of T. cruzi infection was method-dependent, with OM detecting at most 73% of the truly infected T. sordida (assuming 100% detectability with vkDNA-PCR). Across nearly a decade of vector surveillance and control, T. cruzi infection in T. sordida remained <2%, and 0.9). Ziziphus montana occurs in northwestern Yunnan and is primarily limited by temperature seasonality (bio4), elevation, and coldest quarter temperature (bio11). Under future climate scenarios, its suitable habitat is projected to contract by 11.3%-20.4%, with the remaining highly suitable area retreating toward the Jinsha River basin. Bio11 is the primary factor influencing the distribution of Z. mauritiana, and its suitable range is projected to expand by 44.1%-119.1%. Seed heteromorphism was documented for the first time in Z. xiangchengensis, Z. montana, and Z. mairei, classified into Type A (2 carpels) and B (3 carpels) based on carpel number, with Type A seeds dominating (66.0%-94.8%). These two seed types showed significant differences in temperature response and dormancy characteristics. Four Ziziphus species displayed strong physical dormancy, removing the endocarp and cracking the seed coat results in a 100% germination rate, providing a practical solution for large-scale seedling propagation. Based on assessment, Z. montana is more suitable for ecological restoration in the Jinsha River Basin. The population age structure is of the expanding type and serves medicinal, edible, agricultural, ecological restoration, and handicraft purposes. We advocate for community-based "interactive" ecological restoration, using economic incentives to drive ecological recovery. This study contributes to ecological restoration, germplasm conservation, and local economic development. CI - (c) 2026 The Author(s). Ecology and Evolution published by British Ecological Society and John Wiley & Sons Ltd. FAU - Xu, Yulin AU - Xu Y AUID- ORCID: 0009-0009-5334-8303 AD - Key Laboratory of Phytochemistry and Natural Medicines Chinese Academy of Sciences Kunming China. AD - Yunnan Key Laboratory for Integrative Conservation of Plant Species With Extremely Small Populations Kunming Institute of Botany, Chinese Academy of Sciences Kunming China. AD - School of Ecology and Environmental Sciences Yunnan University Kunming China. FAU - Xiong, Zaiqiu AU - Xiong Z AD - Key Laboratory of Phytochemistry and Natural Medicines Chinese Academy of Sciences Kunming China. AD - Yunnan Key Laboratory for Integrative Conservation of Plant Species With Extremely Small Populations Kunming Institute of Botany, Chinese Academy of Sciences Kunming China. AD - School of Ecology and Environmental Sciences Yunnan University Kunming China. FAU - Zhang, Zhiming AU - Zhang Z AD - School of Ecology and Environmental Sciences Yunnan University Kunming China. FAU - Chen, Gao AU - Chen G AD - Key Laboratory of Phytochemistry and Natural Medicines Chinese Academy of Sciences Kunming China. AD - Yunnan Key Laboratory for Integrative Conservation of Plant Species With Extremely Small Populations Kunming Institute of Botany, Chinese Academy of Sciences Kunming China. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Ecol Evol JT - Ecology and evolution JID - 101566408 PMC - PMC13714631 OTO - NOTNLM OT - Jinsha River OT - MaxEnt OT - climate change OT - ecological restoration OT - heteromorphic seeds OT - ziziphus COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/28 CRDT- 2026/10/29 05:58 PHST- 2026/05/12 00:00 [received] PHST- 2026/08/31 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:58 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - ECE374531 [pii] AID - 10.1002/ece3.74531 [doi] PST - epublish SO - Ecol Evol. 2026 Oct 28;16(11):e74531. doi: 10.1002/ece3.74531. eCollection 2026 Nov. PMID- 42953487 OWN - NLM STAT- Publisher LR - 20261029 IS - 2046-2069 (Electronic) IS - 2046-2069 (Linking) DP - 2026 Oct 28 TI - First hyperpolarizability of dithienylethene-based photoswitches: a structure-property relationship study. LID - 10.1039/d6ra08114b [doi] AB - Despite their considerable technological potential, structure-property relationships in intramolecular charge-transfer-based photoswitches remain poorly established. Here, we present a quantum-chemical investigation of the first hyperpolarizability (beta) of a family of dithienylethene (DTE)-based photoswitches, considering static, electro-optic Pockels, and second-harmonic-generation (SHG) responses. Eight donor (D)/acceptor (A) architectures, with and without alkyne (y) pi-extension, are investigated in their open- and closed-ring forms using density functional theory [CAM-B3LYP/6-31+G(d,p)/GD3BJ/(PCM)] in the gas phase and three continuum solvents, with dynamic responses evaluated at 1064 nm. Ring closure generally enhances total first hyperpolarizability (beta (tot)) by restoring through core conjugation, while donor-acceptor asymmetry emerges as the dominant structural factor governing large NLO responses. Alkyne extension further enhances the response when coupled with a push-pull architecture. Solvation modulates both the magnitude and direction of the response, producing pronounced amplification of the closed/open beta (tot) ratio in selected systems and a striking reorientation of the SHG response vector for Dy-yA(open). Time-dependent DFT analysis suggests that the reorientation is associated with a redistribution among nearby excited-state contribution. Importantly, beta (tot) and the directional alignment ratio eta are not necessarily correlated. We therefore introduce R (NLO) as a combined comparative descriptor that captures both response magnitude and directionality. R (NLO) identifies the closed push-pull architectures, particularly Dy-yA(closed), D-yA(closed), and Dy-A(closed), as the most favorable systems among the considered chromophores. Overall, the results show that NLO performance is governed by the coupled effects of donor-acceptor asymmetry, ring closure, pi-extension, and dielectric environment, and that optimization of both response magnitude and directionality is essential for establishing meaningful structure-property relationships in DTE photoswitches. CI - This journal is (c) The Royal Society of Chemistry. FAU - Sarkar, Kanchan AU - Sarkar K AUID- ORCID: 0000-0002-0025-7055 AD - Institut fur Theoretische Chemie, Universitat Ulm Oberberghof 7 89081 Ulm Germany. FAU - Misra, Ramprasad AU - Misra R AUID- ORCID: 0000-0002-1599-404X AD - Institut fur Biologie, Humboldt-Universitat zu Berlin Invalidenstrasse 42 10115 Berlin Germany ramprasad.misra@hu-berlin.de. FAU - Gross, Axel AU - Gross A AUID- ORCID: 0000-0003-4037-7331 AD - Institut fur Theoretische Chemie, Universitat Ulm Oberberghof 7 89081 Ulm Germany. FAU - Sarkar, Rudraditya AU - Sarkar R AUID- ORCID: 0000-0001-7915-8625 AD - Department of Chemistry, GITAM School of Science Hyderabad 502329 Telangana India rudra.smgr@gmail.com rsarkar2@gitam.edu. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - RSC Adv JT - RSC advances JID - 101581657 PMC - PMC13715864 COIS- The authors declare no competing financial interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 PMCR- 2026/10/28 CRDT- 2026/10/29 05:58 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:58 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - d6ra08114b [pii] AID - 10.1039/d6ra08114b [doi] PST - aheadofprint SO - RSC Adv. 2026 Oct 28. doi: 10.1039/d6ra08114b. PMID- 42953484 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2168-8184 (Print) IS - 2168-8184 (Electronic) IS - 2168-8184 (Linking) VI - 18 IP - 9 DP - 2026 Sep TI - When a Seizure Reveals More Than a Hemorrhage: When to Suspect Cerebral Cavernous Malformations. PG - e117023 LID - 10.7759/cureus.117023 [doi] LID - e117023 AB - Cerebral cavernous malformations (CCMs) are low-flow vascular malformations that may be asymptomatic or present with seizures, hemorrhage, headache, or focal neurological deficits. Most are sporadic and solitary, whereas familial CCMs are associated with multiple lesions and autosomal-dominant inheritance. Distinguishing between these forms is clinically relevant for long-term management. We present a 33-year-old woman who presented with generalized tonic-clonic seizures following sleep deprivation. Computed tomography (CT) initially suggested a left frontal intraparenchymal hemorrhage. Magnetic resonance imaging (MRI) subsequently revealed two left frontal Zabramski type II CCMs. Electroencephalography showed cortical dysfunction with paroxysmal activity and contralateral propagation. Antiseizure treatment with phenytoin was initiated and later supplemented with levetiracetam. The patient remained asymptomatic and was referred for tertiary evaluation. This case highlights the diagnostic limitations of CT and the importance of MRI for CCM characterization and detection of multiple lesions. The presence of two cavernomas and a potentially relevant family history raises suspicion for familial CCM, although these findings alone do not establish a hereditary diagnosis. Genetic evaluation and detailed family assessment should therefore be considered. Treatment should be individualized; conservative management is appropriate in selected stable patients, whereas microsurgery and stereotactic radiosurgery are reserved for specific clinical scenarios. CI - Copyright (c) 2026, Uribe-Hernandez et al. FAU - Uribe-Hernandez, Fernando AU - Uribe-Hernandez F AD - Department of Medicine, National Autonomous University of Mexico, Iztacala Faculty of Higher Studies, Tlalnepantla, MEX. FAU - Reyes-Reyes, Jesus AU - Reyes-Reyes J AD - Department of Neurosurgery, Instituto de Seguridad Social del Estado de Mexico y Municipios (ISSEMYM), Regional Hospital Tlalnepantla, Tlalnepantla, MEX. LA - eng PT - Case Reports PT - Journal Article DEP - 20260927 PL - United States TA - Cureus JT - Cureus JID - 101596737 PMC - PMC13714666 OTO - NOTNLM OT - cavernomas OT - cerebral cavernoma OT - cerebral cavernous malformation OT - frontal cavernoma OT - magnetic resonance imaging COIS- Human subjects: Informed consent for treatment and open access publication was obtained or waived by all participants in this study. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/27 CRDT- 2026/10/29 05:58 PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:58 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - 10.7759/cureus.117023 [doi] PST - epublish SO - Cureus. 2026 Sep 27;18(9):e117023. doi: 10.7759/cureus.117023. eCollection 2026 Sep. PMID- 42953478 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-634X (Print) IS - 2296-634X (Electronic) IS - 2296-634X (Linking) VI - 14 DP - 2026 TI - Cellular origins and evolutionary dynamics of plasma cfDNA in solid tumors: integrating germline, tumor-derived, and hematopoietic variation for MRD and treatment guidance. PG - 1928945 LID - 10.3389/fcell.2026.1928945 [doi] LID - 1928945 AB - Plasma cell-free DNA (cfDNA) is a heterogeneous and rapidly changing biological pool derived from malignant cells, normal tissues, and hematopoietic cells. The abundance and molecular composition of circulating tumor DNA (ctDNA) are determined not only by tumor burden but also by tissue architecture, vascular access, cell-death programs, chromatin organization, nuclease processing, systemic clearance, treatment exposure, and clonal evolution. These biological determinants shape the mutation, methylation, fragmentation, nucleosomal, and temporal features exploited by contemporary liquid-biopsy assays. Accurate clinical interpretation therefore requires both sensitive detection and reliable source attribution, particularly the distinction of tumor-derived variants from constitutional germline alterations and clonal hematopoiesis. In this review, we integrate the biological origins and processing of plasma cfDNA with analytical strategies for low-tumor-fraction detection and critically evaluate the evidence supporting postoperative molecular residual disease (MRD), recurrence surveillance, treatment-response assessment, resistance monitoring, and ctDNA-guided therapy across solid tumors. Particular emphasis is placed on distinguishing prognostic validity, treatment-predictive value, and demonstrated clinical utility. Current evidence is strongest in colorectal cancer and, more recently, ctDNA-selected urothelial cancer, whereas routine ctDNA-directed intervention in resected non-small cell lung cancer and breast cancer remains insufficiently validated. By linking cfDNA biology to an explicit hierarchy of clinical evidence, this review defines both the established applications and the major limitations that must be addressed before broader implementation of ctDNA-guided precision oncology. CI - Copyright (c) 2026 Zhao, Wu, Huang, Yang and Zhang. FAU - Zhao, Quanneng AU - Zhao Q AD - Department of Clinical Laboratory, Beijing Anzhen Nanchong Hospital of Capital Medical University and Nanchong Central Hospital, Nanchong, Sichuan, China. FAU - Wu, Chunlei AU - Wu C AD - Department of Blood Transfusion, Beijing Anzhen Nanchong Hospital of Capital Medical University and Nanchong Central Hospital, Nanchong, Sichuan, China. FAU - Huang, Xiao AU - Huang X AD - Department of Clinical Laboratory, Nanchong Hospital of Traditional Chinese Medicine, Nanchong, Sichuan, China. FAU - Yang, Miyuan AU - Yang M AD - Department of Clinical Laboratory, Beijing Anzhen Nanchong Hospital of Capital Medical University and Nanchong Central Hospital, Nanchong, Sichuan, China. FAU - Zhang, Bing AU - Zhang B AD - Department of Clinical Laboratory, Beijing Anzhen Nanchong Hospital of Capital Medical University and Nanchong Central Hospital, Nanchong, Sichuan, China. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Cell Dev Biol JT - Frontiers in cell and developmental biology JID - 101630250 PMC - PMC13714532 OTO - NOTNLM OT - cell-free DNA OT - circulating tumor DNA OT - clonal hematopoiesis OT - germline variant OT - molecular residual disease OT - precision oncology OT - somatic mutation OT - variant-source attribution COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:58 PHST- 2026/07/05 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:58 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1928945 [pii] AID - 10.3389/fcell.2026.1928945 [doi] PST - epublish SO - Front Cell Dev Biol. 2026 Oct 14;14:1928945. doi: 10.3389/fcell.2026.1928945. eCollection 2026. PMID- 42953468 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1663-9812 (Print) IS - 1663-9812 (Electronic) IS - 1663-9812 (Linking) VI - 17 DP - 2026 TI - Corticosteroids in septic shock: mechanisms, heterogeneous treatment effects, and trajectory-guided precision pharmacotherapy. PG - 1962609 LID - 10.3389/fphar.2026.1962609 [doi] LID - 1962609 AB - Corticosteroid therapy in septic shock has evolved from empirical high-dose anti-inflammatory treatment to low-dose adjunctive pharmacotherapy aimed primarily at restoring vascular responsiveness and accelerating shock reversal. Population-level randomized evidence supports a modest average corticosteroid benefit, most consistently reflected in faster shock reversal and earlier vasopressor liberation, whereas mortality effects remain regimen- and population-dependent. This divergence suggests that average treatment effects alone are insufficient to guide individual decisions. We review how pharmacology, randomized evidence, candidate treatment-effect modifiers, and prespecified longitudinal phenotypes may inform the development of individualized corticosteroid strategies, while defining the evidence required before such strategies can guide clinical care. Glucocorticoid and mineralocorticoid receptor biology provides plausible pathways for both benefit and harm, but no single mechanism, cortisol test, transcriptomic endotype, or clinical trajectory is currently validated for routine treatment selection. Candidate enrichment signals must be distinguished as prognostic, predictive, pharmacodynamic, or safety-related and must be available before corticosteroid exposure when used for treatment selection. Observational studies require explicit alignment of eligibility, time zero, treatment strategies, and follow-up; target trial emulation can reduce avoidable temporal bias but cannot substitute for randomization or remove unmeasured confounding. Trajectory-guided enrichment remains investigational: any candidate trajectory used for treatment selection must be assigned or predicted from information available before exposure, and differential treatment effects require explicit evaluation rather than prognostic association alone. Treatment evaluation should extend beyond mortality to shock reversal, organ-support liberation, kidney outcomes, metabolic toxicity, infection, neuromuscular effects, and longer-term function. Clinical decision support should remain a future, clinician-supervised implementation layer that translates validated evidence into time-stamped actions rather than an autonomous prescribing system. Progress toward precision corticosteroid therapy now requires prespecified enrichment hypotheses, direct regimen comparisons, harmonized outcomes, prospective validation, and trials demonstrating clinical utility. CI - Copyright (c) 2026 Jia, Zhao, Liu and Li. FAU - Jia, Zifei AU - Jia Z AD - Second Clinical Medical College of Shandong Medical and Pharmaceutical University (Yantai Affiliated Hospital), Yantai, Shandong, China. FAU - Zhao, Xuanyu AU - Zhao X AD - Second Clinical Medical College of Shandong Medical and Pharmaceutical University (Yantai Affiliated Hospital), Yantai, Shandong, China. FAU - Liu, Xiangtian AU - Liu X AD - Department of Critical Care Medicine, Yantai Yuhuangding Hospital, Yantai, Shandong, China. FAU - Li, Xiaoli AU - Li X AD - Department of Critical Care Medicine, Yantai Yuhuangding Hospital, Yantai, Shandong, China. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Pharmacol JT - Frontiers in pharmacology JID - 101548923 PMC - PMC13714513 OTO - NOTNLM OT - biomarker enrichment OT - corticosteroids OT - fludrocortisone OT - heterogeneous treatment effects OT - hydrocortisone OT - longitudinal trajectories OT - precision pharmacotherapy OT - septic shock COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:57 PHST- 2026/08/09 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:57 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1962609 [pii] AID - 10.3389/fphar.2026.1962609 [doi] PST - epublish SO - Front Pharmacol. 2026 Oct 14;17:1962609. doi: 10.3389/fphar.2026.1962609. eCollection 2026. PMID- 42953467 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2329-4302 (Print) IS - 2329-4310 (Electronic) IS - 2329-4302 (Linking) VI - 13 IP - Suppl 1 DP - 2026 Feb TI - Learning optimized sampling patterns for accelerated MRI reconstruction via diffusion models. PG - S11210 LID - 10.1117/1.JMI.13.S1.S11210 [doi] LID - S11210 AB - PURPOSE: Magnetic resonance imaging (MRI) is a powerful medical imaging modality, but long acquisition times limit throughput, patient comfort, and clinical accessibility. Diffusion-based generative models serve as strong image priors for reducing scan time with accelerated MRI reconstruction and offer robustness across variations in the acquisition model. However, most existing diffusion-based approaches do not exploit the unique ability in MRI to jointly design both the sampling pattern and the reconstruction method. Although prior learning-based approaches have optimized sampling patterns for end-to-end unrolled networks, analogous methods for diffusion-based reconstruction have not been established due to the computational burden of posterior sampling. We propose a method to optimize k -space sampling patterns for accelerated multi-coil MRI reconstruction using diffusion models as priors. APPROACH: We introduce a training objective based on a single-step posterior mean estimate that avoids backpropagation through an expensive iterative reconstruction process. Then, we present a greedy strategy for learning Cartesian sampling patterns that selects informative k -space locations using gradient information from a pre-trained diffusion model while enforcing spatial diversity among samples. RESULTS: Experimental results across multiple anatomies and acceleration factors demonstrate that diffusion models using the optimized sampling patterns achieve higher-quality reconstructions in comparison with using fixed and learned baseline patterns. CONCLUSIONS: We propose a method to learn sampling patterns for accelerating MRI with diffusion-based generative models and posterior sampling reconstruction. CI - (c) 2026 Society of Photo-Optical Instrumentation Engineers (SPIE). FAU - Ravula, Sriram AU - Ravula S AD - The University of Texas at Austin, Chandra Family Department of Electrical and Computer Engineering, Austin, Texas, United States. FAU - Levac, Brett AU - Levac B AD - The University of Texas at Austin, Chandra Family Department of Electrical and Computer Engineering, Austin, Texas, United States. FAU - Arefeen, Yamin AU - Arefeen Y AD - The University of Texas at Austin, Chandra Family Department of Electrical and Computer Engineering, Austin, Texas, United States. AD - MD Anderson Cancer Center, Imaging Physics, Houston, Texas, United States. FAU - Jalal, Ajil AU - Jalal A AD - University of California, Berkeley, Electrical Engineering and Computer Sciences, Berkeley, California, United States. FAU - Dimakis, Alexandros G AU - Dimakis AG AD - University of California, Berkeley, Electrical Engineering and Computer Sciences, Berkeley, California, United States. FAU - Tamir, Jonathan I AU - Tamir JI AUID- ORCID: 0000-0001-9113-9566 AD - The University of Texas at Austin, Chandra Family Department of Electrical and Computer Engineering, Austin, Texas, United States. AD - The University of Texas at Austin, Oden Institute for Computational Engineering and Science, Austin, Texas, United States. AD - Dell Medical School, Department of Diagnostic Medicine, Austin, Texas, United States. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Med Imaging (Bellingham) JT - Journal of medical imaging (Bellingham, Wash.) JID - 101643461 PMC - PMC13715940 OTO - NOTNLM OT - compressed sensing OT - diffusion models OT - generative artificial intelligence OT - magnetic resonance imaging OT - optimal sampling EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2027/10/28 CRDT- 2026/10/29 05:57 PHST- 2026/02/11 00:00 [received] PHST- 2026/08/07 00:00 [revised] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:57 [entrez] PHST- 2027/10/28 00:00 [pmc-release] AID - 260068 [pii] AID - 10.1117/1.JMI.13.S1.S11210 [doi] PST - ppublish SO - J Med Imaging (Bellingham). 2026 Feb;13(Suppl 1):S11210. doi: 10.1117/1.JMI.13.S1.S11210. Epub 2026 Oct 28. PMID- 42953460 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - CD38-targeted therapies in kidney disease: evidence maturity, disease-stage dependence, and biomarker-guided retreatment. PG - 1972506 LID - 10.3389/fmed.2026.1972506 [doi] LID - 1972506 AB - CD38 targeting can reach antibody-secreting cells that persist after B-cell depletion, along with selected natural killer-cell and other immune-cell subsets. This narrative review examines CD38-targeted antibodies in immune-mediated and monoclonal immunoglobulin-related kidney diseases and kidney transplantation. It asks what clinical studies establish, how disease stage may affect response durability, and whether biomarkers can guide retreatment. Evidence differs by agent, regimen, and outcome. The most mature randomized evidence with kidney-organ outcomes comes from daratumumab-based combination therapy in immunoglobulin light-chain (AL) amyloidosis, a plasma-cell disorder. In other kidney and transplant indications, early-phase randomized trials and uncontrolled studies show immunologic, tissue, or proteinuria responses, but long-term kidney or graft benefit remains unproven. Disease duration, previous treatment, and residual tissue injury may help explain variable responses, although treatment selection by disease stage has not been validated. Biomarkers measure distinct aspects of response and can support monitoring; changes alone do not establish pathogenic-cell depletion or justify retreatment. Biomarker-guided dosing was feasible in a selected transplant cohort and requires comparative validation. Clinical interpretation must remain specific to the studied antibody and regimen. Research priorities include trials with kidney and graft outcomes, prospective patient selection, comparisons of treatment strategies, validation of biomarker-guided retreatment, and cumulative safety during repeated therapy. CI - (c) 2026 Feng and Yu. FAU - Feng, Peidong AU - Feng P AD - First School of Clinical Medicine, Shaanxi University of Chinese Medicine, Xianyang, Shaanxi, China. FAU - Yu, Xiaoyong AU - Yu X AD - Department of Nephrology, Shaanxi Provincial Hospital of Traditional Chinese Medicine, Xi'an, Shaanxi, China. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13714534 OTO - NOTNLM OT - CD38 OT - IgA nephropathy OT - antibody-mediated rejection OT - biomarker-guided retreatment OT - lupus nephritis OT - membranous nephropathy OT - plasma cells COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author XY declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:56 PHST- 2026/08/19 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:56 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmed.2026.1972506 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 14;13:1972506. doi: 10.3389/fmed.2026.1972506. eCollection 2026. PMID- 42953458 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2168-8184 (Print) IS - 2168-8184 (Electronic) IS - 2168-8184 (Linking) VI - 18 IP - 9 DP - 2026 Sep TI - Intermuscular Posterolateral Minimally Invasive Open Approach Revisited: Spectrum of Applications and Stabilization Outcomes. PG - e117048 LID - 10.7759/cureus.117048 [doi] LID - e117048 AB - INTRODUCTION AND AIM: Conventional posterior midline open surgical approach (COA) for thoracolumbar spine fixation requires extensive muscular dissection and retraction, resulting in significant complications, including excessive intraoperative bleeding, prolonged postoperative pain, muscle ischemia, and delayed functional recovery. While percutaneous pedicle screw fixation (PPSF) offers reduced invasiveness, it carries inherent risks of radiation exposure and high rates of pedicle screw breach. The posterolateral intermuscular minimally invasive open approach (PLOA) represents an alternative MISS technique that develops an intermuscular plane between paraspinal muscles. This study aimed to evaluate the safety, efficacy, and clinical utility of PLOA pedicle screw fixation (PSF) in various thoracolumbar spine pathologies requiring stabilization without extensive decompression Materials and methods: A retrospective analysis was conducted on 47 patients who underwent PLOA PSF between January 2016 and January 2023 at a tertiary care spinal unit and peripheral unit, with a minimum 24-month follow-up. Inclusion criteria encompassed traumatic fractures, fragility fractures, and pathological fractures, including spondylodiscitis and metastatic disease. Standard preoperative classification systems were applied for traumatic fractures, pathological fractures, and infectious cases. Surgical technique involved 8-10 cm paramedian incisions utilizing region-specific intermuscular posterolateral planes. Intraoperative variables, including operative time, blood loss, and screw accuracy, were documented. Postoperative assessment included hospital length of stay, analgesic requirements compared to COA, complications, and radiographic loss of correction on immediate and final follow-up radiographs using segmental Cobb's angles. Statistical analysis was performed using SPSS version 20.0 (Chicago, IL: SPSS Inc.). RESULTS: A total of 47 patients (mean age 59.1+/-12.04 years; 53%, n=25 males) were enrolled. Primary indications included traumatic fractures (38.2%, n=18), infective spondylodiscitis (36.2%, n=17), pathological tumors (12.7%, n=6), and fragility fractures (8.5%, n=4). A total of 289 pedicle screws were placed (mean 6.14+/-1.43 per patient), with 100% accuracy achieved in both fluoroscopic (51%, n=24 patients) and O-arm navigated (49%, n=23 patients) insertions. Mean operative time was 61.73+/-14.9 min and 59.56+/-11.06 min, with minimal blood loss of 44.78+/-8.45 mL and 45.21+/-8.45 mL. Hospital stay averaged 2.46+/-0.55 days. Postoperatively, 61.7% (n=29) of patients required less analgesia than COA patients, while 29.7% (n=14) required equivalent analgesia and 8.5% (n=4) required more. Loss of correction ranged from 0-20 degrees (mean: 3.46+/-4.92 degrees ) in 30 patients (63.8%), while 17 patients (36.2%) maintained correction. Complications were minimal, with 95.7% (n=45) experiencing no adverse events. CONCLUSION: PLOA represents a viable minimally invasive intermuscular dissection alternative for open thoracolumbar spine stabilization across diverse pathologies, demonstrating superior technical efficiency, minimal blood loss, and rapid postoperative recovery. It is a lower-morbidity approach in resource-limited settings where PPSFs per se are costlier or technology is unavailable in remote settings. CI - Copyright (c) 2026, Krishnan et al. FAU - Krishnan, Ajay AU - Krishnan A AD - Spine Surgery, Bhavnagar Institute of Medical Science, Bhavnagar, IND. AD - Spine Surgery, Stavya Spine Hospital and Research Institute, Ahmedabad, IND. FAU - Dave, Bharatkumar R AU - Dave BR AD - Spine Surgery, Stavya Spine Hospital and Research Institute, Ahmedabad, IND. FAU - Singh, Amritesh AU - Singh A AD - Spine Surgery, Stavya Spine Hospital and Research Institute, Ahmedabad, IND. FAU - Bali, Shiv Kumar AU - Bali SK AD - Orthopedics, All India Institute of Medical Sciences, Rishikesh, IND. FAU - Mayi, Shivanand C AU - Mayi SC AD - Spine Surgery, Stavya Spine Hospital and Research Institute, Ahmedabad, IND. FAU - Rai, Ravi Ranjan AU - Rai RR AD - Spine Surgery, Stavya Spine Hospital and Research Institute, Ahmedabad, IND. FAU - Dave, Mirant B AU - Dave MB AD - Spine Surgery, Stavya Spine Hospital and Research Institute, Ahmedabad, IND. FAU - Panthackel, Mikeson AU - Panthackel M AD - Spine Surgery, Stavya Spine Hospital and Research Institute, Ahmedabad, IND. FAU - Vashishtha, Arjit AU - Vashishtha A AD - Spine Surgery, Stavya Spine Hospital and Research Institute, Ahmedabad, IND. FAU - Adodariya, Yogenkumar AU - Adodariya Y AD - Spine Surgery, Stavya Spine Hospital and Research Institute, Ahmedabad, IND. FAU - Kulkarni, Saurabh S AU - Kulkarni SS AD - Orthopedics, Mahatma Gandhi Medical College and Research Institute, Aurangabad, IND. FAU - Krishnan, Preety AU - Krishnan P AD - Radiology, Stavya Spine Hospital and Research Institute, Ahmedabad, IND. LA - eng PT - Journal Article DEP - 20260928 PL - United States TA - Cureus JT - Cureus JID - 101596737 PMC - PMC13714668 OTO - NOTNLM OT - miss OT - navigated pedicle screw OT - posterolateral approach OT - thoracolumbar instability OT - weaver's approach COIS- Human subjects: Informed consent for treatment and open access publication was obtained or waived by all participants in this study. Institutional Ethics Committee of Stavya Spine Hospital and Research Institute issued approval #SSHRI/CS/NS/PLMISS/AK/106/12.25. The CTRI registration number is CTRI/2026/02/104106. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/28 CRDT- 2026/10/29 05:56 PHST- 2026/03/27 00:00 [received] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:56 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - 10.7759/cureus.117048 [doi] PST - epublish SO - Cureus. 2026 Sep 28;18(9):e117048. doi: 10.7759/cureus.117048. eCollection 2026 Sep. PMID- 42953446 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-861X (Print) IS - 2296-861X (Electronic) IS - 2296-861X (Linking) VI - 13 DP - 2026 TI - Reciprocal modulation between beef protein and wine phenolics during in vitro co-digestion: a proposed ternary complex-mediated mechanism modulated by cooking technique. PG - 1967708 LID - 10.3389/fnut.2026.1967708 [doi] LID - 1967708 AB - BACKGROUND: Beef and red wine form a classic dietary combination, yet how their components reciprocally influence each other during digestion remains unclear. METHODS: This study co-digested steamed, boiled and grilled beef with red wine using the INFOGEST 2.0 in vitro digestion model, combined with molecular docking, to investigate bidirectional modulation between beef proteins and wine phenolics. RESULTS: Wine co-digestion suppressed gastric carbonyl formation by 21.5%-30.8% and preserved free sulfhydryl groups by 27.7%-50.5% across cooking methods. Two-way analysis of variance indicated significant cooking x wine interactions for protein oxidation and amino acid release. The largest improvement occurred in grilled beef, whose gastric digestibility and intestinal amino acid content increased by 20.9% and 67.2%, respectively. Docking simulations predicted that selected phenolics bound to myosin heavy chain and pepsin through hydrogen bonding and hydrophobic interactions, supporting a proposed phenolics-pepsin-myosin ternary complex that may strengthen enzyme-substrate association. Reciprocally, the beef matrix enhanced ABTS antioxidant activity by 24.9% (gastric) and 125.3% (intestinal) relative to wine alone, and was associated with selective phenolic regulation: gastric gallic acid increased by up to 135.7%, whereas intestinal epicatechin and resveratrol rose up to 3.5- and 11.6-fold, respectively. CONCLUSION: These findings support the proposed ternary complex as a plausible molecular hub governing protein-phenolic reciprocity, providing a proposed mechanistic basis for optimizing cooking methods and meat-wine pairing strategies in mixed meals. CI - Copyright (c) 2026 Sun, Xing, Tang, Liu, Liu, Du, Sun and Jiang. FAU - Sun, Ziruo AU - Sun Z AD - School of Food Engineering, Ludong University, Yantai, Shandong, China. FAU - Xing, Shaoyun AU - Xing S AD - School of Food Engineering, Ludong University, Yantai, Shandong, China. FAU - Tang, Weixiang AU - Tang W AD - School of Food Engineering, Ludong University, Yantai, Shandong, China. FAU - Liu, Taihong AU - Liu T AD - School of Food Engineering, Ludong University, Yantai, Shandong, China. FAU - Liu, Kaiyue AU - Liu K AD - School of Food Engineering, Ludong University, Yantai, Shandong, China. FAU - Du, Chao AU - Du C AD - School of Food Engineering, Ludong University, Yantai, Shandong, China. FAU - Sun, Shuyang AU - Sun S AD - School of Food Engineering, Ludong University, Yantai, Shandong, China. FAU - Jiang, Wenguang AU - Jiang W AD - Ningxia Changyu Longyu Estate Co. Ltd., Yinchuan, Ningxia, China. AD - College of Enology, Northwest A&F University, Yangling, Shaanxi, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Nutr JT - Frontiers in nutrition JID - 101642264 PMC - PMC13714533 OTO - NOTNLM OT - beef-wine co-digestion OT - cooking technique OT - phenolic bioaccessibility OT - protein digestibility OT - protein-phenolics interaction COIS- WJ was employed by Ningxia Changyu Longyu Estate Co. Ltd. The remaining author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:56 PHST- 2026/08/14 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:56 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fnut.2026.1967708 [doi] PST - epublish SO - Front Nutr. 2026 Oct 14;13:1967708. doi: 10.3389/fnut.2026.1967708. eCollection 2026. PMID- 42953438 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2168-8184 (Print) IS - 2168-8184 (Electronic) IS - 2168-8184 (Linking) VI - 18 IP - 9 DP - 2026 Sep TI - Hybrid Spinal-General Anesthesia With a Supraglottic Airway in Severe Rheumatoid Arthritis: A Case Report. PG - e117042 LID - 10.7759/cureus.117042 [doi] LID - e117042 AB - Rheumatoid arthritis (RA) can present significant challenges during airway management because of cervical spine involvement, restricted neck mobility, temporomandibular joint disease, and limited mouth opening. We report the perioperative anesthetic management of a patient with severe deforming RA and multiple predictors of an anticipated difficult airway undergoing laparoscopic cholecystectomy. A 54-year-old woman with long-standing RA had a Mallampati grade IV airway, approximately 1.5 cm mouth opening, a short neck, and markedly restricted cervical spine movement. A comprehensive difficult airway strategy was formulated with immediate availability of videolaryngoscopy, fiberoptic bronchoscopy, supraglottic airway devices, and emergency front-of-neck airway equipment. Following spinal anesthesia with 3.2 mL of 0.5% hyperbaric bupivacaine and 15 mug of fentanyl, light general anesthesia was induced while preserving spontaneous ventilation. Neuromuscular blockade was avoided, and a size 3 i-gel second-generation supraglottic airway was successfully inserted on the first attempt. Anesthesia was maintained with sevoflurane while spontaneous ventilation was preserved throughout laparoscopic surgery. Pneumoperitoneum was established gradually with intra-abdominal pressure maintained below 12 mmHg. The procedure was completed uneventfully without desaturation, airway obstruction, or significant hemodynamic instability. The i-gel was removed after recovery of consciousness and adequate airway reflexes. Postoperative recovery was uncomplicated, with satisfactory analgesia and no respiratory, neurological, or airway-related complications. This case illustrates that hybrid spinal-general anesthesia using a second-generation supraglottic airway while preserving spontaneous ventilation may be a useful alternative in carefully selected patients with an anticipated difficult airway. Such an approach requires meticulous preoperative airway assessment, experienced anesthetic management, and immediate availability of appropriate rescue airway equipment. CI - Copyright (c) 2026, Bajaj et al. FAU - Bajaj, Shilpa AU - Bajaj S AD - Anesthesiology, Holy Family Hospital, New Delhi, IND. FAU - Kalshan, Rahul AU - Kalshan R AD - Anesthesiology, Holy Family Hospital, New Delhi, IND. FAU - Shekhawat, Jitendra AU - Shekhawat J AD - Anesthesiology, Holy Family Hospital, New Delhi, IND. FAU - Nidhi, Trisha AU - Nidhi T AD - Anesthesiology, Safdarjung Hospital, New Delhi, IND. LA - eng PT - Case Reports PT - Journal Article DEP - 20260927 PL - United States TA - Cureus JT - Cureus JID - 101596737 PMC - PMC13714519 OTO - NOTNLM OT - case report OT - difficult airway OT - general anesthesia OT - i-gel OT - laparoscopic cholecystectomy OT - rheumatoid arthritis OT - spinal anesthesia OT - spontaneous ventilation OT - supraglottic airway COIS- Human subjects: Informed consent for treatment and open access publication was obtained or waived by all participants in this study. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/27 CRDT- 2026/10/29 05:55 PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:55 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - 10.7759/cureus.117042 [doi] PST - epublish SO - Cureus. 2026 Sep 27;18(9):e117042. doi: 10.7759/cureus.117042. eCollection 2026 Sep. PMID- 42953434 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2090-6625 (Print) IS - 2090-6633 (Electronic) IS - 2090-6633 (Linking) VI - 2026 DP - 2026 TI - Disseminated Klebsiella pneumoniae Infection Causing Multisystem Abscesses and Necrotising Fasciitis in an Immunocompetent Patient: A Case Report. PG - 5938248 LID - 10.1155/crdi/5938248 [doi] LID - 5938248 AB - BACKGROUND: Disseminated Klebsiella pneumoniae infection that concurrently produces a liver abscess, bilateral pulmonary abscesses and necrotising fasciitis is uncommon in patients without overt immunocompromise. Definitive designation of a hypervirulent strain requires the string test and virulence-gene testing, which are not always available at the point of care. CASE PRESENTATION: A previously healthy 48-year-old man, in whom a contaminated lower limb wound was the suspected portal of entry, subsequently developed a liver abscess, multiple bilateral pulmonary abscesses, necrotising soft-tissue infection of the left lower limb and sepsis with circulatory instability. Culture of the liver-abscess aspirate yielded a mucoid K. pneumoniae that was susceptible to carbapenems, ceftazidime/avibactam, amikacin and levofloxacin, among other agents. Because neither the string test nor virulence genes (e.g., rmpA, iucA) were examined, the isolate was described only as having a clinically hypervirulent phenotype based on mucoid colony morphology and the multisite invasive pattern. Stenotrophomonas maltophilia and multidrug-resistant Acinetobacter baumannii were recovered sequentially from bronchoalveolar lavage fluid and sputum and were interpreted, in context, as airway colonisation or ventilator-associated tracheobronchitis. After multisite percutaneous drainage, repeated debridement with vacuum sealing drainage, staged antimicrobial therapy and organ support, the patient recovered and was discharged; at the 3-month follow-up, the lesions had largely resolved without recurrence. CONCLUSION: This case suggests that multisite metastatic abscesses in an immunocompetent individual should raise suspicion for invasive K. pneumoniae infection. Early, adequate source control together with rational antimicrobial stewardship appears central to a favourable outcome. The conclusions are limited by the single-case design and the absence of molecular confirmation of hypervirulence. CI - Copyright (c) 2026 Qi Yi et al. Case Reports in Infectious Diseases published by John Wiley & Sons Ltd. FAU - Yi, Qi AU - Yi Q AUID- ORCID: 0009-0005-7438-4873 AD - Department of Emergency Medicine Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University) Changsha Hunan China hunnu.edu.cn. FAU - Huang, Ying AU - Huang Y AD - Department of Emergency Medicine Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University) Changsha Hunan China hunnu.edu.cn. FAU - Cao, Huanxin AU - Cao H AD - Department of Emergency Medicine Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University) Changsha Hunan China hunnu.edu.cn. FAU - Zhang, Xingwen AU - Zhang X AUID- ORCID: 0000-0002-7603-1255 AD - Department of Emergency Medicine Hunan Provincial People's Hospital (The First Affiliated Hospital of Hunan Normal University) Changsha Hunan China hunnu.edu.cn. LA - eng PT - Journal Article DEP - 20261028 PL - Egypt TA - Case Rep Infect Dis JT - Case reports in infectious diseases JID - 101573243 PMC - PMC13714568 OTO - NOTNLM OT - Klebsiella pneumoniae OT - liver abscess OT - necrotising fasciitis OT - sepsis OT - source control COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/28 CRDT- 2026/10/29 05:55 PHST- 2026/05/02 00:00 [received] PHST- 2026/09/09 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:55 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - CRDI5938248 [pii] AID - 10.1155/crdi/5938248 [doi] PST - epublish SO - Case Rep Infect Dis. 2026 Oct 28;2026:5938248. doi: 10.1155/crdi/5938248. eCollection 2026. PMID- 42953428 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1663-9812 (Print) IS - 1663-9812 (Electronic) IS - 1663-9812 (Linking) VI - 17 DP - 2026 TI - Advanced drug delivery and PBPK modelling as translational tools for precision therapies in juvenile idiopathic arthritis. PG - 1917187 LID - 10.3389/fphar.2026.1917187 [doi] LID - 1917187 AB - Patients with persistent forms of juvenile idiopathic arthritis (JIA) often report limited therapeutic benefit from currently available treatments. Despite substantial advances in the management of rheumatoid arthritis (RA) and related disorders over recent decades, treatment strategies for JIA remain insufficiently defined. The application of advanced drug delivery systems holds promise in addressing these limitations by enabling controlled and sustained drug release over extended periods. Other integrative approaches such as novel physiologically based pharmacokinetic (PBPK) modelling have demonstrated to be a critical tool in optimizing pharmacotherapy in pediatric populations, particularly by improving dose selection and predicting drug disposition. This review summarizes current disease-modifying antirheumatic drugs (DMARDs) for JIA management and critically examines the current, still largely prospective, integration of emerging drug delivery systems with PBPK modelling, highlighting existing translational examples alongside key opportunities and challenges for precision therapies in this field. CI - Copyright (c) 2026 Cuquerella-Gilabert, Martin-Sabroso, Zarzoso-Foj, Aparicio-Blanco, Balsa, Torres-Suarez, Mangas-Sanjuan and Rodriguez-Nogales. FAU - Cuquerella-Gilabert, Marina AU - Cuquerella-Gilabert M AD - Department of Pharmacy and Pharmaceutical Technology and Parasitology, University of Valencia, Valencia, Spain. AD - Interuniversity Research Institute for Molecular Recognition and Technological Development, Polytechnic University of Valencia - University of Valencia, Valencia, Spain. FAU - Martin-Sabroso, Cristina AU - Martin-Sabroso C AD - Department of Pharmaceutics and Food Technology, Faculty of Pharmacy, Complutense University of Madrid, Madrid, Spain. AD - Institute of Industrial Pharmacy, Complutense University of Madrid, Madrid, Spain. FAU - Zarzoso-Foj, Javier AU - Zarzoso-Foj J AD - Department of Pharmacy and Pharmaceutical Technology and Parasitology, University of Valencia, Valencia, Spain. AD - Interuniversity Research Institute for Molecular Recognition and Technological Development, Polytechnic University of Valencia - University of Valencia, Valencia, Spain. FAU - Aparicio-Blanco, Juan AU - Aparicio-Blanco J AD - Department of Pharmaceutics and Food Technology, Faculty of Pharmacy, Complutense University of Madrid, Madrid, Spain. AD - Institute of Industrial Pharmacy, Complutense University of Madrid, Madrid, Spain. FAU - Balsa, Alejandro AU - Balsa A AD - Rheumatology Unit, La Paz University Hospital, Madrid, Spain. FAU - Torres-Suarez, Ana Isabel AU - Torres-Suarez AI AD - Department of Pharmaceutics and Food Technology, Faculty of Pharmacy, Complutense University of Madrid, Madrid, Spain. AD - Institute of Industrial Pharmacy, Complutense University of Madrid, Madrid, Spain. FAU - Mangas-Sanjuan, Victor AU - Mangas-Sanjuan V AD - Department of Pharmacy and Pharmaceutical Technology and Parasitology, University of Valencia, Valencia, Spain. AD - Interuniversity Research Institute for Molecular Recognition and Technological Development, Polytechnic University of Valencia - University of Valencia, Valencia, Spain. FAU - Rodriguez-Nogales, Carlos AU - Rodriguez-Nogales C AD - Department of Pharmaceutics and Food Technology, Faculty of Pharmacy, Complutense University of Madrid, Madrid, Spain. AD - Institute of Industrial Pharmacy, Complutense University of Madrid, Madrid, Spain. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Pharmacol JT - Frontiers in pharmacology JID - 101548923 PMC - PMC13714579 OTO - NOTNLM OT - DMARDs OT - PBPK OT - TMDD OT - arthritis OT - controlled release OT - drug delivery systems OT - juvenile idiopathic arthritis OT - long-acting formulations COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:54 PHST- 2026/06/23 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:54 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1917187 [pii] AID - 10.3389/fphar.2026.1917187 [doi] PST - epublish SO - Front Pharmacol. 2026 Oct 14;17:1917187. doi: 10.3389/fphar.2026.1917187. eCollection 2026. PMID- 42953423 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2980-2156 (Electronic) IS - 2980-2156 (Linking) VI - 48 IP - 5 DP - 2026 Oct TI - Effectiveness and Safety of Suction-Assisted Technologies in Pediatric Endourological Stone Surgery: A Systematic Review by the EAU-YAU Pediatric Urology Working Group. PG - 469-476 LID - 10.14744/cpr.2026.89214 [doi] AB - OBJECTIVE: Although new suction/vacuum technologies have been tested in adults in recent years, they remain uncommon in pediatric practice. This systematic review aimed to analyze the existing literature on the effectiveness and safety of new suction technologies for pediatric endourological stone interventions. MATERIALS AND METHODS: We conducted a systematic review (PROSPERO CRD420261335417) following the PRISMA 2020 and Cochrane guidelines, searching PubMed, Embase, MEDLINE, Web of Science, and Scopus to identify all published reports on new suction/vacuum technologies for pediatric endourological stone interventions through February 2026. The primary outcomes were intraoperative and postoperative complications and stone-free rates (SFRs). Secondary outcomes included operative time, hospital stay, and the need for ancillary procedures. We used the MINORS tool to assess the risk of bias in nonrandomized cohort studies. RESULTS: The review included a total of 367 patients (370 renal units) aged 1-17 years who underwent endourological stone surgery. The vacuum-assisted mini-PCNL group had a higher SFR of 81.3-100% and lower complication and reintervention rates of 0-27.8% and 0-12.5%, respectively, compared with conventional mini-PCNL. Furthermore, a higher SFR of 85.7-100% was observed with RIRS using FANS-UAS compared with an SFR of 60-88% in children who underwent conventional RIRS. The former group also had a lower reintervention rate (0-9.5% vs. 0-40%) and fewer grade III-IV complications (0% vs. 8.7%). CONCLUSION: Current evidence, although limited and mainly based on observational studies, suggests that suction-assisted technologies may improve stone clearance and maintain acceptable safety profiles in selected pediatric endourological stone interventions. However, the absence of randomized trials, heterogeneous outcome definitions, and limited long-term follow-up prevent definitive conclusions. CI - Copyright (c) Author(s). FAU - Selvi, Ismail AU - Selvi I AD - Department of Pediatric Urology, Health Science University Gazi Yasargil Training and Research Hospital, Diyarbakir, Turkiye. FAU - Baydilli, Numan AU - Baydilli N AD - Department of Pediatric Urology, Erciyes University Faculty of Medicine, Kayseri, Turkiye. FAU - Donmez, Muhammet Irfan AU - Donmez MI AD - Division of Pediatric Urology, Department of Urology, Istanbul University, Istanbul Faculty of Medicine, Istanbul, Turkiye. FAU - Khondker, Adree AU - Khondker A AD - Division of Urology, Department of Surgery, University of Toronto, Ontario, Canada. FAU - Madarriaga, Yesica Quiroz AU - Madarriaga YQ AD - Division of Pediatric Urology, Department of Urology, Fundacio Puigvert, Barcelona, Spain. FAU - Gernhold, Christa AU - Gernhold C AD - Department of Pediatric Urology, Ordensklinikum Linz Hospital of the Sisters of Charity, Linz, Austria. FAU - Pierucci, Ugo Maria AU - Pierucci UM AD - Department of Pediatric Surgery, Buzzi Children's Hospital, Milan, Italy. FAU - Lammers, Rianne Janna Marie AU - Lammers RJM AD - Department of Pediatric Urology, University Medical Center Groningen, Groningen, Netherlands. FAU - Tatanis, Vasileios AU - Tatanis V AD - Department of Pediatric Urology, University Hospital of Patras, Patras, Greece. FAU - Marco, Beatriz Banuelos AU - Marco BB AD - Department of Urology, Hospital Universitari de Girona Doctor Josep Trueta, Girona, Spain. FAU - t'Hoen, Lisette Aimee AU - t'Hoen LA AD - Department of Pediatric Urology, Sophia Children's Hospital, Erasmus University Medical Center, Rotterdam, Netherlands. LA - eng PT - Journal Article PT - Review DEP - 20261008 PL - Turkey TA - J Clin Pract Res JT - Journal of clinical practice and research JID - 9919014495706676 PMC - PMC13715658 OTO - NOTNLM OT - Children OT - RIRS OT - flexible and navigable ureteral access sheath OT - kidney stones OT - percutaneous nephrolithotomy OT - suction OT - vacuum COIS- The authors have no conflicts of interest to declare. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/08 CRDT- 2026/10/29 05:54 PHST- 2026/05/21 00:00 [received] PHST- 2026/06/29 00:00 [revised] PHST- 2026/07/06 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:54 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - JCPR-48-469 [pii] AID - 10.14744/cpr.2026.89214 [doi] PST - epublish SO - J Clin Pract Res. 2026 Oct 8;48(5):469-476. doi: 10.14744/cpr.2026.89214. eCollection 2026 Oct. PMID- 42953420 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2090-6447 (Print) IS - 2090-6455 (Electronic) VI - 2026 DP - 2026 TI - Extensive Mandibular Bone Regeneration Following Iodoform and Lyophilized Bone Grafting in a Patient Initially Indicated for Hemimandibulectomy. PG - 5757136 LID - 10.1155/crid/5757136 [doi] LID - 5757136 AB - Mandibular cysts arise from various etiologies and generally exhibit benign characteristics, with favorable treatment outcomes. Nevertheless, extensive cystic lesions may involve cortical bone, leading to substantial bone loss, reduced mechanical integrity of the mandible, and an increased risk of pathological fractures. In severe cases, hemimandibulectomy may become a clinical option, potentially resulting in functional and aesthetic impairment and requiring complex mandibular reconstruction. Therefore, conservative regenerative strategies aimed at preserving mandibular continuity may provide substantial benefits for patient recovery and quality of life. In this case report, we present a successful approach in which a mandible severely affected by benign cyst-induced bone loss was preserved and extensively regenerated over a 6-year follow-up period. Following preoperative endodontic management with iodoform, the cyst was completely enucleated, and the resulting extensive bone defect was filled with lyophilized bovine bone combined with iodoform and covered with collagen membranes and a titanium mesh. Long-term radiographic follow-up demonstrated progressive increases in radiopacity and integration of the treated area, whereas computed tomography at 5.5 years revealed restoration of cortical bone continuity and extensive mandibular remodeling. At 6 years, the treated region exhibited radiopacity similar to that of the contralateral preserved mandibular region. These findings suggest that, in carefully selected extensive benign mandibular lesions, a conservative regenerative approach may preserve mandibular continuity and potentially avoid extensive mandibular resection. CI - Copyright (c) 2026 Renato Zacura et al. Case Reports in Dentistry published by John Wiley & Sons Ltd. FAU - Zacura, Renato AU - Zacura R AD - Surgery Department Postgraduation Program of Biomaterials and Regenerative Medicine Faculty of Medical Sciences and Health Pontifical Catholic University of Sao Paulo Sao Paulo Brazil pucsp.br. FAU - Hausen, Moema A AU - Hausen MA AUID- ORCID: 0000-0002-7131-4045 AD - Surgery Department Postgraduation Program of Biomaterials and Regenerative Medicine Faculty of Medical Sciences and Health Pontifical Catholic University of Sao Paulo Sao Paulo Brazil pucsp.br. FAU - Gerenutti, Marli AU - Gerenutti M AD - Surgery Department Postgraduation Program of Biomaterials and Regenerative Medicine Faculty of Medical Sciences and Health Pontifical Catholic University of Sao Paulo Sao Paulo Brazil pucsp.br. LA - eng PT - Journal Article DEP - 20261028 PL - Egypt TA - Case Rep Dent JT - Case reports in dentistry JID - 101573242 PMC - PMC13714575 OTO - NOTNLM OT - absorbable bone biomaterials OT - hemimandibulectomy OT - iodoform OT - mandible regeneration OT - mandibular cysts COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/28 CRDT- 2026/10/29 05:54 PHST- 2026/01/21 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:54 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - CRID5757136 [pii] AID - 10.1155/crid/5757136 [doi] PST - epublish SO - Case Rep Dent. 2026 Oct 28;2026:5757136. doi: 10.1155/crid/5757136. eCollection 2026. PMID- 42953419 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1179-1594 (Print) IS - 1179-1594 (Electronic) IS - 1179-1594 (Linking) VI - 19 DP - 2026 TI - Self-Medication: A Critical Review of Clinical, Economic, and Health-System Trade-Offs. PG - 649932 LID - 10.2147/RMHP.S649932 [doi] LID - 649932 AB - BACKGROUND: Self-medication is an established component of self-care, but its implications for patients and health systems are not uniformly beneficial or harmful. This critical review examines the clinical, economic and health-system trade-offs. METHODS: PubMed, Scopus and Google Scholar informed a focused critical synthesis of 52 sources. The original literature check was conducted in August 2026; revision-stage source verification continued through 13 October 2026. Selection and descriptive critical appraisal were undertaken by one author. RESULTS: Pharmacist-supported minor-ailment care improved selected clinical and referral outcomes. An Australian trial reported symptom resolution or relief in 94% versus 88% with usual pharmacy care, with no statistically significant difference in reconsultation; its economic evaluation estimated an additional AUD7.14 per patient and AUD2277 per quality-adjusted life-year. In a Ugandan hospital-based study, self-medication was associated with admission attributed to adverse drug reactions (adjusted odds ratio 2.27; 95% confidence interval 1.14-4.55). Pharmacy interventions reduced non-prescription antibiotic dispensing in some settings, but persistent supply and limited clinical-outcome evidence constrained interpretation. Overall, benefits and risks varied by medicine, patient characteristics, professional support and healthcare access; evidence did not establish a single net clinical or economic benefit across these different practices. CONCLUSION: Policy should support suitable non-prescription care through medicine-safety checks and defined referral criteria. In low- and middle-income countries, higher-risk-medicine restrictions should accompany affordable diagnosis and treatment. Local evaluation should measure adverse outcomes, completed referrals and household and health-system costs. Focused single-author selection limits coverage and causal interpretation. CI - (c) 2026 Sabrie. FAU - Sabrie, Zainab AU - Sabrie Z AUID- ORCID: 0000-0003-1494-7652 AD - Faculty of Medicine and Health Sciences, SIMAD University, Mogadishu, Somalia. AD - Department of Pharmacy, Dr Sumait Hospital, SIMAD University, Mogadishu, Somalia. LA - eng PT - Journal Article PT - Review DEP - 20261024 PL - England TA - Risk Manag Healthc Policy JT - Risk management and healthcare policy JID - 101566264 PMC - PMC13715647 OTO - NOTNLM OT - antimicrobial resistance OT - community pharmacy OT - health economics OT - non-prescription medicines OT - self-care OT - self-medication COIS- The author declares no competing interests in this work. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/24 CRDT- 2026/10/29 05:54 PHST- 2026/08/22 00:00 [received] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:54 [entrez] PHST- 2026/10/24 00:00 [pmc-release] AID - 649932 [pii] AID - 10.2147/RMHP.S649932 [doi] PST - epublish SO - Risk Manag Healthc Policy. 2026 Oct 24;19:649932. doi: 10.2147/RMHP.S649932. eCollection 2026. PMID- 42953415 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1179-7258 (Print) IS - 1179-7258 (Electronic) IS - 1179-7258 (Linking) VI - 17 DP - 2026 TI - Virtual Reality in Surgical Education: Evidence, Applications, Implementation Challenges, and Future Directions. PG - 642019 LID - 10.2147/AMEP.S642019 [doi] LID - 642019 AB - This narrative review examines the role of virtual reality (VR) in surgical education, including practice, assessment, rehearsal, and collaboration. It considers applications across neurosurgery, ophthalmology, orthopedics, spine surgery, laparoscopy, robotic surgery, and oral and maxillofacial surgery. Targeted searches of PubMed and Crossref covered January 1990 through July 20, 2026. Titles and abstracts were screened for relevance, and study selection was cross-checked by two authors; systematic reviews, meta-analyses, randomized trials, multicenter studies, and specialty-specific validation studies were prioritized. No independent formal assessment of evidence quality was performed. Evidence most consistently supports short-term knowledge and technical-skill acquisition, particularly in laparoscopy, ophthalmology, and selected orthopedic procedures. Transfer to supervised operative performance is supported for specific tasks, but patient-level evidence remains limited and should not be generalized across platforms or specialties. Effective use depends on curricular design, deliberate practice, feedback, proficiency standards, and validity evidence appropriate to the intended educational use. Persistent barriers include incomplete haptic fidelity, cybersickness, unequal access, total cost of ownership, faculty readiness, heterogeneous outcome measures, and limited external validation. Artificial intelligence may support automated assessment and adaptive instruction, while hybrid simulation, patient-specific modeling, remote learning, and multiplayer environments may broaden educational reach. These innovations require transparent validation, human oversight, and equity-focused implementation. VR should be adopted as a targeted component of blended surgical education rather than as a replacement for other simulation methods or supervised clinical experience. CI - (c) 2026 Huang et al. FAU - Huang, Peng AU - Huang P AUID- ORCID: 0000-0003-3011-3359 AD - Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, People's Republic of China. AD - Clinical Research Center for Thyroid Disease in Hunan Province, Changsha, Hunan, 410008, People's Republic of China. AD - Hunan Provincial Engineering Research Center for Thyroid and Related Diseases Treatment Technology, Changsha, Hunan, 410008, People's Republic of China. FAU - Zeng, Jia-Xing AU - Zeng JX AD - Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, People's Republic of China. FAU - Zhan, Zhi-Jun AU - Zhan ZJ AD - Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, People's Republic of China. FAU - Tang, Neng AU - Tang N AD - Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, People's Republic of China. FAU - Chang, Shi AU - Chang S AD - Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, 410008, People's Republic of China. AD - Clinical Research Center for Thyroid Disease in Hunan Province, Changsha, Hunan, 410008, People's Republic of China. AD - Hunan Provincial Engineering Research Center for Thyroid and Related Diseases Treatment Technology, Changsha, Hunan, 410008, People's Republic of China. AD - National Clinical Research Center for Geriatric Disorders (Xiangya Hospital), Changsha, Hunan, 410008, People's Republic of China. LA - eng PT - Journal Article PT - Review DEP - 20261024 PL - New Zealand TA - Adv Med Educ Pract JT - Advances in medical education and practice JID - 101562700 PMC - PMC13715529 OAB - Virtual reality allows surgical learners to practice in an interactive computer-generated environment before performing procedures on patients. It can help learners understand three-dimensional anatomy, rehearse procedural steps, and receive feedback on their movements and errors. The most consistent evidence supports improvements in knowledge and technical skills. Some studies also show transfer to supervised surgery, especially in laparoscopy and cataract training, but these findings do not apply to every device or procedure. Programs should choose VR only when it solves a defined educational problem. They should combine it with expert feedback, other simulation methods, and supervised clinical training. Cost, motion sickness, limited touch sensation, accessibility, and the quality of performance measures must also be considered. Artificial intelligence and remote team training may make VR more personalized and widely available, but these uses still need transparent testing and educator oversight. OABL- eng OTO - NOTNLM OT - clinical transfer OT - competency assessment OT - haptic feedback OT - immersive learning OT - simulation-based medical education COIS- The authors report no conflicts of interest in this work. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/24 CRDT- 2026/10/29 05:53 PHST- 2026/07/20 00:00 [received] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:53 [entrez] PHST- 2026/10/24 00:00 [pmc-release] AID - 642019 [pii] AID - 10.2147/AMEP.S642019 [doi] PST - epublish SO - Adv Med Educ Pract. 2026 Oct 24;17:642019. doi: 10.2147/AMEP.S642019. eCollection 2026. PMID- 42953406 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2297-055X (Print) IS - 2297-055X (Electronic) IS - 2297-055X (Linking) VI - 13 DP - 2026 TI - Saint-Bonnet Doppler waveform classification system: a scoping review and evidence-based appraisal using AGREE II and AGREE-REX. PG - 1870182 LID - 10.3389/fcvm.2026.1870182 [doi] LID - 1870182 AB - BACKGROUND: Doppler waveform interpretation is central to the diagnosis and surveillance of lower limb peripheral arterial disease (PAD). Despite widespread clinical use of duplex ultrasound (DUS) and hand-held Doppler (HHD), waveform terminology remains highly variable and non-standardised across institutions. The Saint-Bonnet classification, proposed by the French College of Teachers in Vascular Medicine (CEMV), offers a structured eight-category morphological framework mapped to haemodynamic severity. Its evidence base and methodological quality have never been formally appraised. METHODS: A scoping review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR). MEDLINE (Ovid) and Embase (Ovid) were searched from 1946 to November 2025. Studies describing, applying, validating, or evaluating the Saint-Bonnet classification were eligible. Data were extracted using a standardised form. Methodological quality was appraised using AGREE II (six domains) and AGREE-REX (three domains), independently by two reviewers (H.A. and F.A.). RESULTS: Seven studies met the inclusion criteria; all published between 2017 and 2022 from a single French vascular research group. The Saint-Bonnet classification achieved a categorisation rate of 98.2% across 1,033 waveforms, outperforming alternative systems. Inter-rater reliability was moderate (kappa approximately 0.55). Waveform grade independently correlated with maximal walking distance, and waveform-guided ankle-brachial index (ABI) selection achieved near-perfect agreement with guideline standards (kappa up to 1.00). Automated neural network classification reached kappa = 0.79, approaching expert agreement. AGREE II appraisal revealed high scores for Clarity of Presentation and Scope & Purpose (97% and 83%), but low scores for Rigour of Development and Applicability (44% and 40%). AGREE-REX demonstrated high Clinical Applicability and Implementability. CONCLUSION: The Saint-Bonnet classification is a promising and practical framework for Doppler waveform interpretation, with consistent findings across the available studies. Low AGREE II scores for Rigour of Development and Applicability reflect gaps in its documented development and reporting rather than evidence against its clinical performance. However, all supporting evidence originates from a single, non-independent French research group, and the classification has not undergone external validation; broader adoption should await independent, multicentre confirmation of these findings. CI - (c) 2026 Alodayni, Alodaini, Davies and Normahani. FAU - Alodayni, Hamidah Mabruk AU - Alodayni HM AD - Section of Vascular Surgery, Department of Surgery and Cancer, Imperial College London, London, United Kingdom. AD - Department of Radiology and Medical Imaging, College of Applied Medical Sciences, Majmaah University, Al-Majmaah, Saudi Arabia. FAU - Alodaini, Fatimah AU - Alodaini F AD - Institute of Epidemiology and Health Care, University College London (UCL), London, United Kingdom. FAU - Davies, Alun H AU - Davies AH AD - Section of Vascular Surgery, Department of Surgery and Cancer, Imperial College London, London, United Kingdom. AD - Imperial Vascular Unit, Imperial College Healthcare NHS Trust, London, United Kingdom. FAU - Normahani, Pasha AU - Normahani P AD - Section of Vascular Surgery, Department of Surgery and Cancer, Imperial College London, London, United Kingdom. AD - Imperial Vascular Unit, Imperial College Healthcare NHS Trust, London, United Kingdom. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Cardiovasc Med JT - Frontiers in cardiovascular medicine JID - 101653388 PMC - PMC13714507 OTO - NOTNLM OT - AGREE II OT - AGREE-REX OT - Doppler waveform OT - Saint-Bonnet OT - duplex ultrasound OT - hand-held Doppler OT - peripheral arterial disease OT - scoping review COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:53 PHST- 2026/04/30 00:00 [received] PHST- 2026/09/13 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:53 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fcvm.2026.1870182 [doi] PST - epublish SO - Front Cardiovasc Med. 2026 Oct 14;13:1870182. doi: 10.3389/fcvm.2026.1870182. eCollection 2026. PMID- 42953404 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-634X (Print) IS - 2296-634X (Electronic) IS - 2296-634X (Linking) VI - 14 DP - 2026 TI - Metabolic control of methylation landscapes in cancer growth and plasticity: targeting the epigenetic-metabolic axis with natural compounds and emerging therapies. PG - 1943655 LID - 10.3389/fcell.2026.1943655 [doi] LID - 1943655 AB - Cancer cell plasticity enables tumor cells to dynamically transition between diverse phenotypic states, including epithelial-mesenchymal plasticity stemness, dormancy, lineage switching, immune evasion, and therapy-resistant phenotypes. Although metabolic reprogramming and epigenetic regulation have each been recognized as hallmarks of cancer, the mechanistic framework linking metabolic adaptation to dynamic cell-state transitions remains incompletely understood. In this Review, we propose that methylation serves as a major mechanistic interface through which metabolic rewiring can influence adaptive cancer cell plasticity. We discuss how one-carbon metabolism regulates the availability of the universal methyl donor S-adenosylmethionine (SAM), thereby influencing DNA methylation, histone methylation, and multiple forms of RNA methylation, with N6-methyladenosine (m(6)A) representing the principal RNA modification discussed in this review. Rather than responding uniformly to metabolic change, these methylation systems represent distinct but metabolically connected regulatory layers that share dependence on one-carbon metabolism while differing in their biochemical sensitivities and regulatory outputs. Net methylation states, however, are also shaped by metabolically constrained demethylation, including alpha-ketoglutarate-, oxygen-, Fe(2+)-, and flavin adenine dinucleotide (FAD)-dependent eraser pathways. Together, these metabolically responsive writing and erasing processes influence transcriptional stability, chromatin accessibility, and post-transcriptional regulation during reversible cell-state transitions. We further examine how environmental and therapeutic stresses, including hypoxia, nutrient limitation, immune surveillance, and anticancer therapy, reshape methylation programs through metabolic adaptation, and how such remodeling may support phenotypic flexibility and survival under selective pressure. Building upon these advances, we propose the concept of the adaptive methylation landscape, a perturbation-responsive, state-associated, and potentially reversible distribution of methylation states that contributes to shaping the spectrum of cellular states accessible during tumor evolution. Finally, we discuss emerging therapeutic strategies targeting the metabolic-methylation axis and highlight how single-cell multi-omics, spatial epigenomics, functional epigenome editing, and AI may enable dynamic mapping of adaptive methylation landscapes. We propose that cancer cell plasticity is not merely a genetic phenomenon but can also be shaped by metabolically responsive methylation programs, providing a testable conceptual framework for future precision epigenetic therapies. CI - Copyright (c) 2026 Liu, Yu, Li, Chen, Shi, Li, Wei and Luo. FAU - Liu, Qi AU - Liu Q AD - Changchun University of Chinese Medicine, Changchun, China. FAU - Yu, Ying AU - Yu Y AD - Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, China. FAU - Li, Wenbo AU - Li W AD - Changchun University of Chinese Medicine, Changchun, China. FAU - Chen, Xinxu AU - Chen X AD - School of Basic Medicine, Faculty of Medicine, Dalian University of Technology, Dalian, China. FAU - Shi, Zekai AU - Shi Z AD - Changchun University of Chinese Medicine, Changchun, China. FAU - Li, Gang AU - Li G AD - China Academy of Chinese Medical Sciences Guang'anmen Hospital, Beijing, China. FAU - Wei, Shibo AU - Wei S AD - School of Basic Medicine, Faculty of Medicine, Dalian University of Technology, Dalian, China. FAU - Luo, Zongjian AU - Luo Z AD - Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, China. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Cell Dev Biol JT - Frontiers in cell and developmental biology JID - 101630250 PMC - PMC13714509 OTO - NOTNLM OT - S-adenosylmethionine OT - cancer cell plasticity OT - epigenetic reprogramming OT - methylation OT - therapy resistance COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:53 PHST- 2026/07/21 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:53 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1943655 [pii] AID - 10.3389/fcell.2026.1943655 [doi] PST - epublish SO - Front Cell Dev Biol. 2026 Oct 14;14:1943655. doi: 10.3389/fcell.2026.1943655. eCollection 2026. PMID- 42953402 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Current evidence of flexible ureteroscopic laser lithotripsy under local anesthesia: feasibility, safety, and clinical application. PG - 1942070 LID - 10.3389/fmed.2026.1942070 [doi] LID - 1942070 AB - Flexible ureteroscopic laser lithotripsy (FURL) is commonly performed under general or neuraxial anesthesia, but local anesthesia (LA) has been explored as an alternative for selected patients. This structured narrative review summarizes current evidence for LA-FURL while explicitly separating LA alone, LA combined with systemic analgesia or conscious/moderate sedation, peripheral regional blocks, and neuraxial anesthesia. An updated literature search identified a small but growing body of direct LA-FURL evidence, consisting predominantly of retrospective or single-arm clinical series together with recent larger prospective cohorts. These studies suggest that FURL can be completed and tolerated under LA in selected patients, with stone-free rates generally in the range reported by individual series; however, differences in stone burden, anesthesia protocols, access-sheath technology, imaging modality, timing, and definitions of stone-free status preclude a pooled estimate or a claim of equivalence to general anesthesia. Observational complication profiles are encouraging, but comparative safety and non-inferiority versus general anesthesia have not been established. Technical measures such as careful patient preparation, gentle instrumentation, suction-assisted access sheaths, attention to intrarenal pressure, and selective pre-stenting may be useful, but most have not been validated as LA-FURL-specific standards. Evidence from ureteroscopy under conscious sedation is considered separately because it represents a distinct anesthetic strategy. Overall, LA-FURL is a promising option for appropriately selected patients and experienced teams, particularly when avoidance of general or neuraxial anesthesia is desirable, but the current evidence base remains heterogeneous and largely nonrandomized. Prospective comparative studies with standardized definitions of procedural completion, stone-free status, pain, complications, patient-reported outcomes, and cost are required before broader recommendations can be made. CI - (c) 2026 Chen, Xing, Shi and Lai. FAU - Chen, Hongbo AU - Chen H AD - Department of Urology, The Third Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China. FAU - Xing, Shiyu AU - Xing S AD - Department of Urology, The Third Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China. FAU - Shi, Shenglong AU - Shi S AD - Department of Urology, The Third Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China. FAU - Lai, Shufan AU - Lai S AD - Department of Anesthesiology, The Third Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi, China. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13714410 OTO - NOTNLM OT - feasibility OT - flexible ureteroscopic laser lithotripsy OT - flexible ureteroscopy OT - local anesthesia OT - safety OT - urolithiasis COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:53 PHST- 2026/07/19 00:00 [received] PHST- 2026/09/06 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:53 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmed.2026.1942070 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 14;13:1942070. doi: 10.3389/fmed.2026.1942070. eCollection 2026. PMID- 42953394 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1752-4571 (Print) IS - 1752-4571 (Electronic) IS - 1752-4571 (Linking) VI - 19 IP - 11 DP - 2026 Nov TI - Legacy of Recurrent Gene Flow and Evidence of Independent Evolution of ALS Resistance Mutations in Weedy Rice. PG - e70342 LID - 10.1111/eva.70342 [doi] LID - e70342 AB - Weedy rice (Oryza spp.) provides a unique model for studying adaptive evolution because herbicide resistance can arise through both crop-to-weed gene flow and independent mutational events. In Brazil, nearly two decades of cultivation of imidazolinone (IMI)-resistant rice have imposed intense selection for acetolactate synthase (ALS) inhibitors resistance. This study investigated how historical gene flow and independent evolution have shaped the contemporary landscape of ALS resistance mutations in Brazilian weedy rice. Specifically, we characterized the diversity and distribution of ALS resistance mutations and developed rapid allele-specific molecular markers for major resistance alleles. A total of 200 weedy rice accessions collected from the major rice-growing region of Brazil were screened for IMI resistance and genotyped using newly developed SNAP (single nucleotide amplified polymorphism) qPCR markers targeting the IMI rice-derived mutations Ala122Thr, Ser653Asn, and Gly654Glu. Samples negative for these mutations were sequenced, and dose-response assays were conducted with imazethapyr, penoxsulam, nicosulfuron, and cloransulam-methyl. Ninety-three percent of the accessions were resistant to IMI herbicides. The ALS mutation Gly654Glu, present in rice cultivars used until 2006, was found in 15.5% of weedy rice resistant accessions, Ala122Thr became the dominant mutation (54.5%), whereas Ser653Asn occurred at an intermediate frequency (16.0%), indicating a temporal shift in the genetic basis of ALS resistance. Sequencing further identified the ALS substitutions Ala205Val, Trp574Leu, and Ser653Thr, which were not associated with commercial IMI-resistant rice cultivars, indicating ongoing independent evolution of target-site resistance. Trp574Leu conferred broad cross-resistance to all ALS-inhibiting herbicides evaluated, whereas the remaining mutations displayed mutation-specific resistance spectra. The SNAP markers showed high specificity and sensitivity, enabling reliable high-throughput detection of resistance alleles. Together, our findings demonstrate that the contemporary ALS resistance landscape in Brazilian weedy rice has been shaped mainly by repeated crop-to-weed gene flow, but also ongoing independent mutational events that continue to diversify resistance under herbicide selection. CI - (c) 2026 The Author(s). Evolutionary Applications published by John Wiley & Sons Ltd. FAU - Turra, Guilherme Menegol AU - Turra GM AUID- ORCID: 0000-0002-4806-7613 AD - Crop Science Departament Federal University of Rio Grande Do Sul Porto Alegre RS Brazil. FAU - Angonese, Paula Sinigaglia AU - Angonese PS AUID- ORCID: 0000-0001-7947-8178 AD - Crop Science Departament Federal University of Rio Grande Do Sul Porto Alegre RS Brazil. FAU - Rudell, Eduardo Carlos AU - Rudell EC AUID- ORCID: 0000-0002-4296-2318 AD - Crop Science Departament Federal University of Rio Grande Do Sul Porto Alegre RS Brazil. FAU - Tasca, Vinicius Ferrari AU - Tasca VF AUID- ORCID: 0009-0007-6424-816X AD - Crop Science Departament Federal University of Rio Grande Do Sul Porto Alegre RS Brazil. FAU - Markus, Catarine AU - Markus C AUID- ORCID: 0000-0002-5330-3502 AD - Crop Science Departament Federal University of Rio Grande Do Sul Porto Alegre RS Brazil. FAU - Merotto Junior, Aldo AU - Merotto Junior A AUID- ORCID: 0000-0002-1581-0669 AD - Crop Science Departament Federal University of Rio Grande Do Sul Porto Alegre RS Brazil. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Evol Appl JT - Evolutionary applications JID - 101461828 PMC - PMC13714446 OTO - NOTNLM OT - ALS inhibitors OT - Oryza sativa OT - SNAP OT - molecular markers OT - target-site resistance COIS- The authors declare that the molecular markers and plant materials carrying the ALS resistance mutations described in this manuscript are covered by patent application BR1020260149047. G.M.T., P.S.A., and A.M.J. are named as inventors on this application. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/27 CRDT- 2026/10/29 05:52 PHST- 2026/07/18 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:52 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - EVA70342 [pii] AID - 10.1111/eva.70342 [doi] PST - epublish SO - Evol Appl. 2026 Oct 27;19(11):e70342. doi: 10.1111/eva.70342. eCollection 2026 Nov. PMID- 42953391 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1179-1314 (Print) IS - 1179-1314 (Electronic) IS - 1179-1314 (Linking) VI - 18 DP - 2026 TI - Clinicopathological Profile, Treatment Patterns and Documented Clinical Outcomes of Women with Breast Cancer at Shendi Hospital, Sudan: A Retrospective, Single-Center, Hospital-Based Cross-Sectional Study. PG - 650507 LID - 10.2147/BCTT.S650507 [doi] LID - 650507 AB - BACKGROUND: Breast cancer is the most common cancer in Sudan, yet hospital-based data from outside Khartoum are scarce. We described the clinicopathological profile, treatment and documented outcomes of women with breast cancer at Shendi Hospital and explored factors associated with recurrence, distant metastasis and global well-being. METHODS: In this retrospective, single-center, cross-sectional review, we abstracted records of 100 women with histologically confirmed invasive breast cancer managed in 2021-2025. Distant metastasis was defined as documented extranodal disease, and global well-being as a single good/fair/poor rating recorded in clinical notes. Associations were examined with exact tests and three pre-specified Firth penalized logistic regression models (four covariates each) with bootstrap validation; all inferential analyses were exploratory. RESULTS: Median age was 50 years (IQR 44-55). Stage III/IV disease was recorded in 72%, tumors >2 cm in 97% and nodal involvement in 73%. Receptor status was undocumented in 69%; all 31 documented results were hormone-receptor-negative (18 triple-negative, 13 HER2-positive), yet 16 of these women had recorded hormonal therapy. Mastectomy was performed in 65%; chemotherapy, radiotherapy and hormonal therapy were recorded in 99%, 48% and 45%. Recurrence was documented in 19/98 women (19%) and extranodal metastasis in 30/93 (32%); no deaths were recorded and follow-up duration was unknown. Tumors >5 cm were associated with recurrence (adjusted odds ratio 3.81, 95% CI 1.28-11.61), and extranodal metastasis with a fair well-being rating (5.64, 2.01-17.20); nodal status, economic status and age were not associated with any outcome after adjustment. Recorded treatment co-occurred with recurrence, but treatment timing was undocumented, precluding causal interpretation. CONCLUSION: Women at this center presented with advanced disease, and receptor documentation was largely absent and internally inconsistent. These exploratory associations need confirmation in prospective studies with complete receptor testing, dated treatment records and active follow-up. CI - (c) 2026 Ali et al. FAU - Ali, Elryah I AU - Ali EI AD - Department of Medical Laboratory Technology, College of Applied Medical Sciences, Northern Border University, Arar, Saudi Arabia. FAU - Abusail Mustafa, Saadalnour AU - Abusail Mustafa S AD - Department of Clinical Laboratory Science, College of Applied Medical Sciences, Najran University, Najran, Saudi Arabia. FAU - Elzein, Husham O AU - Elzein HO AD - Department of Medical Laboratory Technology, College of Applied Medical Sciences, Northern Border University, Arar, Saudi Arabia. FAU - Andarawi, Mohamed O AU - Andarawi MO AD - Department of Pathology, College of Medicine, King Khalid University, Abha, Saudi Arabia. FAU - Zaroog, Mohammed Suleiman AU - Zaroog MS AD - Department of Community Health, Faculty of Applied Medical Sciences, Northern Border University, Arar, Saudi Arabia. AD - Center for Health Research, Northern Border University, Arar, Saudi Arabia. FAU - Abass, Awad-Elkareem AU - Abass AE AUID- ORCID: 0000-0003-0833-3769 AD - Department of Medical Laboratory Technology, College of Applied Medical Sciences, Northern Border University, Arar, Saudi Arabia. FAU - Attaelmanan, Gamila Ali AU - Attaelmanan GA AD - Department of Hematology, Faculty of Medical Laboratory Sciences, Al-Neelain University, Khartoum, Sudan. FAU - Yasin, Fatima Mohamed Osman AU - Yasin FMO AUID- ORCID: 0009-0002-0300-6144 AD - Faculty of Medical Laboratory Sciences, University of Gadarif, Gadarif, Sudan. FAU - Alfeel, Ayman Hussein AU - Alfeel AH AD - Department of Medical Laboratory Sciences, College of Health Sciences, Gulf Medical University, Ajman, United Arab Emirates. FAU - Ismail, Marwan AU - Ismail M AUID- ORCID: 0000-0001-5692-1632 AD - Department of Medical Laboratory Sciences, College of Health Sciences, Gulf Medical University, Ajman, United Arab Emirates. LA - eng PT - Journal Article DEP - 20261024 PL - New Zealand TA - Breast Cancer (Dove Med Press) JT - Breast cancer (Dove Medical Press) JID - 101591856 PMC - PMC13715536 OTO - NOTNLM OT - Breast cancer OT - Sudan OT - hormone receptor status OT - late-stage presentation OT - medical record review OT - recurrence COIS- The authors declare that they have no competing interests in this work. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/24 CRDT- 2026/10/29 05:52 PHST- 2026/08/21 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:52 [entrez] PHST- 2026/10/24 00:00 [pmc-release] AID - 650507 [pii] AID - 10.2147/BCTT.S650507 [doi] PST - epublish SO - Breast Cancer (Dove Med Press). 2026 Oct 24;18:650507. doi: 10.2147/BCTT.S650507. eCollection 2026. PMID- 42953387 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2048-7177 (Print) IS - 2048-7177 (Electronic) IS - 2048-7177 (Linking) VI - 14 IP - 11 DP - 2026 Nov TI - Integrated Multi-Omics Analysis of Polygonatum odoratum Polysaccharide in D-Galactose-Treated Mice Reveals Changes in Oxidative Stress Markers and Gut Microbiota. PG - e72500 LID - 10.1002/fsn3.72500 [doi] LID - e72500 AB - Age-related changes are associated with oxidative stress, metabolic dysregulation, and alterations in the gut microbiota. Although Polygonatum odoratum polysaccharide (POP) has shown antioxidant and neuroprotective effects, its associations with gut bacterial composition, circulating metabolites, and tissue gene expression remain unclear. Using a D-galactose-treated mouse model, we assessed hippocampal histology and oxidative stress markers and performed bacterial 16S rRNA sequencing, untargeted serum metabolomics, and hippocampal and colonic transcriptomic analyses. POP treatment was associated with improved hippocampal histology, reduced malondialdehyde levels, and increased glutathione levels and superoxide dismutase activity. It was also associated with changes in selected gut bacterial taxa and serum lipid metabolites, lower expression of NF-kappaB-related genes (Btk, Lat, and Tnfrsf13c) in the colon, and lower Ninj2 expression in the hippocampus. Cross-omics analysis identified correlations among these bacterial, metabolite, and gene-expression features. These findings identify candidate gut-serum-tissue associations accompanying the effects of POP in D-galactose-treated mice. Functional studies are needed to determine whether any of these changes mediate its effects. CI - (c) 2026 The Author(s). Food Science & Nutrition published by Wiley Periodicals LLC. FAU - Li, Wenjian AU - Li W AUID- ORCID: 0009-0008-3715-2274 AD - School of Pharmacy Hunan University of Chinese Medicine Changsha China. AD - Hunan Province Engineering Technology Research Center of Bioactive Substance Discovery of Chinese Medicine Changsha China. FAU - Huang, Yan AU - Huang Y AD - Hunan Province Hospital of Integrated Traditional Chinese and Western Medicine (Affiliated Hospital of Hunan Academy of Chinese Medicine) Changsha China. FAU - Zhang, Wenfang AU - Zhang W AD - School of Pharmacy Hunan University of Chinese Medicine Changsha China. FAU - Li, Shunxiang AU - Li S AD - School of Pharmacy Hunan University of Chinese Medicine Changsha China. AD - Hunan Province Engineering Technology Research Center of Bioactive Substance Discovery of Chinese Medicine Changsha China. AD - Hunan Province Sino-US International Joint Research Center for Therapeutic Drugs of Senile Degenerative Diseases Changsha China. FAU - Li, Lisong AU - Li L AUID- ORCID: 0009-0007-6305-6510 AD - School of Informatics Hunan University of Chinese Medicine Changsha China. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Food Sci Nutr JT - Food science & nutrition JID - 101605473 PMC - PMC13715506 OTO - NOTNLM OT - D-galactose OT - Polygonatum odoratum polysaccharide OT - aging OT - multi-omics analysis OT - oxidative stress COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/28 CRDT- 2026/10/29 05:52 PHST- 2025/12/13 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:52 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - FSN372500 [pii] AID - 10.1002/fsn3.72500 [doi] PST - epublish SO - Food Sci Nutr. 2026 Oct 28;14(11):e72500. doi: 10.1002/fsn3.72500. eCollection 2026 Nov. PMID- 42953385 OWN - NLM STAT- Publisher LR - 20261029 IS - 2046-2069 (Electronic) IS - 2046-2069 (Linking) DP - 2026 Oct 28 TI - Synthesis, antitumor evaluation, and in silico investigation of pyridine-based pyrimidotriazine and triazolopyrimidine derivatives as potential EGFR-targeted anticancer agents. LID - 10.1039/d6ra07493f [doi] AB - Cancer remains a major global health challenge and one of the leading causes of mortality worldwide. In the present study, a new series of fused nitrogen-containing heterocyclic derivatives was synthesized from a pyridine-based pyrimidinethione key synthon. Hydrazinolysis followed by cyclo-condensation and condensation reactions afforded structurally diverse pyrimidotriazine, triazolopyrimidine, hydrazone, and pyrazolopyrazole derivatives. The antiproliferative activities of the synthesized compounds were evaluated against human lung carcinoma (A549) and human prostate carcinoma (PC3) cell lines, with WI-38 normal human lung fibroblasts used to assess selectivity. Compounds 6 and 8 emerged as the most promising cell-based candidates, displaying the highest activities against A549 and PC3 cells, respectively, together with favorable selectivity toward cancer cells relative to WI-38 normal cells. To investigate a possible mechanism underlying the observed antiproliferative effects, the most active derivatives (6-8) were screened for EGFR kinase inhibition. Compound 7 exhibited the strongest inhibitory activity, surpassing sorafenib and revealing a clear structure-activity relationship within the series. To gain structural insight into target recognition, molecular docking studies were conducted against EGFR (PDB ID: 1M17) and cyclin-dependent kinase 2 (CDK2; PDB ID: 2A4L). The docking protocol was validated by re-docking of the corresponding co-crystallized ligands, demonstrating satisfactory reproduction of the experimental binding modes. Compounds 6-8 adopted favorable binding orientations within the ATP-binding pockets of both enzymes. Compound 7 displayed the most favorable EGFR binding profile, whereas compound 6 showed the strongest predicted affinity toward CDK2. The observed interactions emphasized the importance of molecular planarity, aromaticity, hydrogen-bonding capability, and hydrophobic contacts in stabilizing ligand-enzyme complexes. In silico ADME analysis revealed generally acceptable drug-likeness and pharmacokinetic characteristics, with compound-dependent variations in lipophilicity, gastrointestinal absorption, blood-brain barrier permeability, and P-glycoprotein recognition. Collectively, these findings identify compounds 6-8 as promising anticancer candidates, with compounds 6 and 8 representing potent and selective cell-based leads and compound 7 emerging as a promising EGFR-directed scaffold for further optimization. Furthermore, this work expands the chemical space of fused nitrogen heterocycles and provides a useful platform for the development of kinase-targeted anticancer agents. CI - This journal is (c) The Royal Society of Chemistry. FAU - Ramadan, Sayed K AU - Ramadan SK AUID- ORCID: 0000-0003-2743-6544 AD - Chemistry Department, Faculty of Science, Ain Shams University Cairo 11566 Egypt sayed.karam2008@sci.asu.edu.eg. FAU - Abdelrahman, Ali H AU - Abdelrahman AH AD - Chemistry Department, Faculty of Science, Ain Shams University Cairo 11566 Egypt sayed.karam2008@sci.asu.edu.eg. FAU - Hegazy, Mohamed A AU - Hegazy MA AUID- ORCID: 0000-0002-2961-9312 AD - Petrochemicals Department, Egyptian Petroleum Research Institute (EPRI) Cairo 11727 Egypt. FAU - Labena, Ahmed AU - Labena A AD - Processes Design and Development Department, Egyptian Petroleum Research Institute (EPRI) Cairo 11727 Egypt. FAU - Gomha, Sobhi M AU - Gomha SM AUID- ORCID: 0000-0002-7739-2837 AD - Department of Chemistry, Faculty of Science, Islamic University of Madinah Madinah 42351 Saudi Arabia smgomha@iu.edu.sa. FAU - Zaki, Magdi E A AU - Zaki MEA AD - Department of Chemistry, Faculty of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU) Riyadh 11623 Saudi Arabia. FAU - Azab, Mohammad E AU - Azab ME AD - Chemistry Department, Faculty of Science, Ain Shams University Cairo 11566 Egypt sayed.karam2008@sci.asu.edu.eg. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - RSC Adv JT - RSC advances JID - 101581657 PMC - PMC13715544 COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 PMCR- 2026/10/28 CRDT- 2026/10/29 05:52 PHST- 2026/08/12 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:52 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - d6ra07493f [pii] AID - 10.1039/d6ra07493f [doi] PST - aheadofprint SO - RSC Adv. 2026 Oct 28. doi: 10.1039/d6ra07493f. PMID- 42953372 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1098-1004 (Electronic) IS - 1059-7794 (Print) IS - 1059-7794 (Linking) VI - 2026 DP - 2026 TI - Integrative Multiomics Mendelian Randomization Prioritizes Endoplasmic Reticulum Stress-Related Genes Associated With Gastric Cancer Risk. PG - 3845963 LID - 10.1155/humu/3845963 [doi] LID - 3845963 AB - BACKGROUND: Emerging research indicates connections between endoplasmic reticulum (ER) stress and gastric cancer (GC), but related genetic factors remain unclear. This study is aimed at prioritizing ER stress-related genes associated with GC risk using multiomics data. METHODS: We integrated GC GWAS data from FinnGen (discovery) and United Kingdom Biobank (replication) cohorts with QTL datasets, including mQTLs, eQTLs, and pQTLs. Summary-data-based Mendelian randomization (SMR) and colocalization analyses were performed to prioritize candidate genes, followed by characterization using transcriptomic profiling (GSE118916), methylation-expression correlation analysis (TCGA-STAD), and single-cell RNA-seq analysis (GSE270680). RESULTS: SMR analyses identified 83 mQTL, 10 eQTL, and 2 pQTL signals associated with GC risk. Among these, colocalization evidence was observed for 47 mQTL, 5 eQTL, and 2 pQTL signals. Transcriptomic analysis showed that PCSK6, YOD1, and RNF5 exhibited expression changes directionally consistent with their SMR associations with GC risk, whereas CTNNBIP1 showed an inconsistent expression pattern. PCSK6 was supported by mQTL, eQTL, and mQTL-eQTL evidence but lacked colocalization. YOD1, identified through pQTL-SMR, showed strong colocalization evidence (PPH4 = 0.95). RNF5 was highlighted via eQTL-SMR but lacked colocalization support. CTNNBIP1 was supported by mQTL, eQTL, and mQTL-eQTL evidence and colocalization, with a significant methylation-expression correlation observed in TCGA-STAD. Single-cell RNA-seq revealed that PCSK6 was predominantly expressed in fibroblasts, YOD1 in epithelial cells and mast cells, and RNF5 broadly across fibroblasts, epithelial cells, and selected immune cells. CONCLUSIONS: This multiomics study prioritized PCSK6, YOD1, and RNF5 as ER stress-related candidate genes showing genetic associations with GC risk and directionally consistent expression patterns, with single-cell analysis indicating distinct cell-type-specific expression patterns. These findings offer useful context for future functional and mechanistic investigations of the prioritized candidate genes. CI - Copyright (c) 2026 Dong Yang et al. Human Mutation published by John Wiley & Sons Ltd. FAU - Yang, Dong AU - Yang D AUID- ORCID: 0000-0002-9007-9909 AD - Department of Gastrosurgery Liaoning Cancer Hospital and Institute Shenyang China lnszl.com. FAU - Liu, Yuanlin AU - Liu Y AUID- ORCID: 0000-0002-1553-0341 AD - Department of Gastrosurgery Liaoning Cancer Hospital and Institute Shenyang China lnszl.com. FAU - Meng, Xiangyu AU - Meng X AUID- ORCID: 0000-0002-6876-0028 AD - Department of Gastrosurgery Liaoning Cancer Hospital and Institute Shenyang China lnszl.com. FAU - Wang, Chao AU - Wang C AUID- ORCID: 0009-0002-2220-8550 AD - Department of Gastrosurgery Liaoning Cancer Hospital and Institute Shenyang China lnszl.com. FAU - Yu, Zhiyong AU - Yu Z AUID- ORCID: 0000-0002-9362-5311 AD - Department of General Surgery Yingkou Central Hospital Yingkou China. FAU - Zhang, Tao AU - Zhang T AUID- ORCID: 0000-0001-7939-9090 AD - Department of Gastrosurgery Liaoning Cancer Hospital and Institute Shenyang China lnszl.com. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Hum Mutat JT - Human mutation JID - 9215429 SB - IM MH - Humans MH - *Stomach Neoplasms/genetics/metabolism MH - *Endoplasmic Reticulum Stress/genetics MH - Quantitative Trait Loci MH - *Mendelian Randomization Analysis/methods MH - Genome-Wide Association Study MH - Multiomics MH - *Genetic Predisposition to Disease MH - Gene Expression Profiling MH - Gene Expression Regulation, Neoplastic MH - Polymorphism, Single Nucleotide PMC - PMC13714348 OTO - NOTNLM OT - Mendelian randomization analysis OT - colocalization OT - endoplasmic reticulum stress OT - gastric cancer OT - multiomics OT - single-cell RNA sequencing COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/27 CRDT- 2026/10/29 05:51 PHST- 2025/09/30 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:51 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - HUMU3845963 [pii] AID - 10.1155/humu/3845963 [doi] PST - epublish SO - Hum Mutat. 2026 Oct 27;2026:3845963. doi: 10.1155/humu/3845963. eCollection 2026. PMID- 42953369 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2197-1153 (Print) IS - 2197-1153 (Electronic) IS - 2197-1153 (Linking) VI - 13 IP - 4 DP - 2026 Oct TI - Remnant preservation and stump suturing in anterior cruciate ligament reconstruction reduce tibial tunnel widening without compromising tunnel placement: A computed tomography-based randomised controlled trial. PG - e70962 LID - 10.1002/jeo2.70962 [doi] LID - e70962 AB - PURPOSE: Tunnel widening remains a concern following anterior cruciate ligament reconstruction because it may adversely affect graft incorporation and complicate revision surgery. Remnant preservation has been proposed as a strategy to reduce tunnel widening, but its effect on postoperative tunnel remodelling remains uncertain. This study aimed to determine whether remnant preservation with tibial stump suturing reduces tunnel widening without compromising tunnel placement accuracy. METHODS: In this prospective randomised controlled trial, 179 patients undergoing primary anterior cruciate ligament (ACL) reconstruction were randomised to remnant-preserving reconstruction with tibial stump suturing (n = 88) or conventional stump resection (n = 91). Standardised three-dimensional computed tomography was performed at 2 weeks and 1 year postoperatively. Tibial and femoral tunnel widening were calculated as the percentage change in tunnel diameter between scans, and tunnel placement accuracy was assessed using the computed tomography-based quadrant method. The incidence of clinically significant tunnel widening (>20%) was also compared. RESULTS: Baseline tunnel diameters were comparable between groups. At 1-year follow-up, remnant-preserving ACL reconstruction resulted in significantly less tibial tunnel widening than stump resection (14.58 +/- 4.49% vs. 19.74 +/- 4.36%, p < 0.001), with a lower incidence of clinically significant widening (>20%) (11.4% vs. 25.3%; relative risk, 0.45; p = 0.016). Femoral tunnel widening was similar between groups (18.23 +/- 2.92% vs. 18.35 +/- 2.59%, p = 0.766). No clinically relevant differences in tunnel placement were observed between groups. CONCLUSION: Remnant-preserving ACL reconstruction with tibial stump suturing selectively reduces tibial tunnel widening at 12 months without compromising anatomic tunnel placement accuracy. These findings support the use of remnant preservation when preservation of tibial tunnel integrity is a surgical consideration. LEVEL OF EVIDENCE: Level I. TRIAL REGISTRATION: ClinicalTrials.gov, NCT07236593; registration date: November 15, 2025 (retrospectively registered). CI - (c) 2026 The Author(s). Journal of Experimental Orthopaedics published by John Wiley & Sons Ltd on behalf of European Society of Sports Traumatology, Knee Surgery and Arthroscopy. FAU - El-Desouky, Mahmoud AU - El-Desouky M AUID- ORCID: 0000-0001-5558-4566 AD - Department of Orthopaedic Surgery, Faculty of Medicine Cairo University Cairo Egypt. FAU - Elkalyoby, Ahmed Samir AU - Elkalyoby AS AUID- ORCID: 0000-0002-3184-7402 AD - Department of Orthopaedic Surgery, Faculty of Medicine Cairo University Cairo Egypt. FAU - Rashwan, Amr Samir AU - Rashwan AS AUID- ORCID: 0009-0006-3125-2130 AD - Department of Orthopaedic Surgery, Faculty of Medicine Cairo University Cairo Egypt. FAU - Gaballah, Amr Ibrahim AU - Gaballah AI AD - Department of Orthopaedic Surgery, Faculty of Medicine Cairo University Cairo Egypt. FAU - Abdelrazek, Begad Hesham AU - Abdelrazek BH AD - Department of Orthopaedic Surgery, Faculty of Medicine Cairo University Cairo Egypt. LA - eng SI - ClinicalTrials.gov/NCT07236593 PT - Journal Article DEP - 20261028 PL - United States TA - J Exp Orthop JT - Journal of experimental orthopaedics JID - 101653750 PMC - PMC13715493 OTO - NOTNLM OT - ACL reconstruction OT - computed tomography OT - remnant preservation OT - tunnel placement accuracy OT - tunnel widening COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/28 CRDT- 2026/10/29 05:51 PHST- 2026/07/31 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:51 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - JEO270962 [pii] AID - 10.1002/jeo2.70962 [doi] PST - epublish SO - J Exp Orthop. 2026 Oct 28;13(4):e70962. doi: 10.1002/jeo2.70962. eCollection 2026 Oct. PMID- 42953367 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2214-7500 (Electronic) IS - 2214-7500 (Linking) VI - 17 DP - 2026 Dec TI - Acute and 28-day repeated-dose oral toxicity of a synthetic 3-hydroxyindolin-2-one derivative in BALB/c mice. PG - 102354 LID - 10.1016/j.toxrep.2026.102354 [doi] LID - 102354 AB - Preclinical safety evaluation of newly synthesized bioactive molecules is a crucial step in the drug development process. The acute and subacute oral toxicity of the synthesized 3-Hydroxyindolin-2-one derivative, 7-bromo-3-hydroxy-3-(2-oxo-2-(p-tolyl)ethyl)indolin-2-one (4b), was evaluated in BALB/c mice using a sequential-dose acute oral toxicity/tolerability assessment and a 28-day repeated-dose study. For the acute oral assessment, compound 4b was administered sequentially at 2000, 1000, and 300 mg/kg body weight, followed by a 14-day observation period. Mortality was observed at 2000 and 1000 mg/kg, whereas no mortality was observed at 300 mg/kg, and subacute toxicity was determined by oral doses of 15, 30, and 60 mg/kg per day for 28 days. To determine the body weight, hematological parameters, biochemical parameters, gross pathological examination, and histopathological evaluation of major organs were performed for the synthesized compound. No mortality was observed at 300 mg/kg during the acute observation period or during the 28-day repeated-dose study, and no treatment-related gross pathological changes were observed at the examined doses. Several statistically significant differences and deviations from the reference intervals were observed among individual hematological and biochemical parameters following repeated administration. However, these changes did not demonstrate a consistent dose-response pattern across sexes and were not accompanied by concordant clinical, gross pathological, or histopathological findings. Histopathological evaluation of selected organs in the vehicle-control and high-dose groups showed well-preserved tissue architecture, with no treatment-related lesions identified in the examined tissues. Together, these results indicate that the synthesized compound 4b caused no mortality or clear treatment-related hematological, biochemical, gross pathological, or histopathological effects at the administered doses. CI - (c) 2026 The Authors. FAU - Mondal, Nakibul Islam AU - Mondal NI AD - Department of Pharmacology, SRM College of Pharmacy, Faculty of Medicine and Health Sciences, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu, Tamil Nadu, 603203, India. FAU - Vellapandian, Chitra AU - Vellapandian C AD - Department of Pharmacology, SRM College of Pharmacy, Faculty of Medicine and Health Sciences, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu, Tamil Nadu, 603203, India. LA - eng PT - Journal Article DEP - 20261015 PL - Ireland TA - Toxicol Rep JT - Toxicology reports JID - 101630272 PMC - PMC13715401 OTO - NOTNLM OT - 3-hydroxyindolin-2-one derivative OT - Acute & Subacute oral toxicity OT - BALB/c mice OT - Histopathology OT - Preclinical safety evaluation COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/15 CRDT- 2026/10/29 05:51 PHST- 2026/08/05 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:51 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - S2214-7500(26)00156-3 [pii] AID - 102354 [pii] AID - 10.1016/j.toxrep.2026.102354 [doi] PST - epublish SO - Toxicol Rep. 2026 Oct 15;17:102354. doi: 10.1016/j.toxrep.2026.102354. eCollection 2026 Dec. PMID- 42953365 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-462X (Print) IS - 1664-462X (Electronic) IS - 1664-462X (Linking) VI - 17 DP - 2026 TI - Metabolomics-based comparison of metabolite profiles in Capparis spinosa L. from different habitats and associated soil factors. PG - 1914134 LID - 10.3389/fpls.2026.1914134 [doi] LID - 1914134 AB - INTRODUCTION: Capparis spinosa L. is a distinctive plant with edible and medicinal value, yet the factors influencing the accumulation of its effective components across diverse habitats remain unclear. This study aims to profile fruit metabolites and explore the association with rhizosphere soil environments. METHODS: Using LC-MS/MS-based untargeted metabolomics (six biological replicates per habitat), fruit metabolites from three habitats in northern Xinjiang (Urumqi, Karamay, and Ili) were profiled. Partial least squares discriminant analysis (PLS-DA) identified differential metabolites. 16S rRNA sequencing analyzed rhizosphere soil microbial communities, and correlation analyses linked fruit metabolites with soil metabolites and microbiota. RESULTS: A total of 1,739 metabolites were identified. PLS-DA revealed clear habitat separation, with 454, 340, and 411 differential metabolites in pairwise comparisons. Sixteen anti-inflammatory compounds (flavonoids, terpenoids, etc.) were candidate quality-associated metabolites, predominantly highest in Urumqi. Soil bacterial/fungal composition differed significantly among habitats, with Rubrobacterand Gaiella enriched in Urumqi. Correlation analysis linked 16 fruit metabolites (e.g., resveratrol, kaempferol) to 10 soil metabolites and specific soil genera. DISCUSSION: Habitat-driven differences in soil microbial communities and metabolite levels are associated with bioactive component abundance in C. spinosa fruit, offering targets for habitat selection and microbiome-informed cultivation. Note that LC-MS/MS signal intensities are semi-quantitative; reported differences reflect relative abundance rather than absolute content. CI - Copyright (c) 2026 Xiayifu, Zhao, Bai, Liu and Wang. FAU - Xiayifu, Xiakela AU - Xiayifu X AD - College of Ecology and Environment, Xinjiang University, Urumqi, Xinjiang, China. FAU - Zhao, Ruli AU - Zhao R AD - College of Life Science and Technology, Xinjiang University, Urumqi, Xinjiang, China. FAU - Bai, Ziyi AU - Bai Z AD - College of Ecology and Environment, Xinjiang University, Urumqi, Xinjiang, China. FAU - Liu, Xiaoying AU - Liu X AD - College of Life Science and Technology, Xinjiang University, Urumqi, Xinjiang, China. FAU - Wang, Xinhui AU - Wang X AD - College of Ecology and Environment, Xinjiang University, Urumqi, Xinjiang, China. AD - College of Life Science and Technology, Xinjiang University, Urumqi, Xinjiang, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Plant Sci JT - Frontiers in plant science JID - 101568200 PMC - PMC13715592 OTO - NOTNLM OT - Capparis spinosa L. OT - different habitats OT - metabolomics OT - quality differences OT - rhizosphere soil COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:51 PHST- 2026/06/22 00:00 [received] PHST- 2026/08/31 00:00 [revised] PHST- 2026/09/07 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:51 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpls.2026.1914134 [doi] PST - epublish SO - Front Plant Sci. 2026 Oct 14;17:1914134. doi: 10.3389/fpls.2026.1914134. eCollection 2026. PMID- 42953362 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 0970-9134 (Print) IS - 0973-7723 (Electronic) IS - 0970-9134 (Linking) VI - 42 IP - 11 DP - 2026 Nov TI - Developing proficiency in a transcatheter aortic valve replacement program: lessons from a single academic center's learning curve. PG - 1194-1203 LID - 10.1007/s12055-026-02249-x [doi] AB - PURPOSE: Aortic stenosis (AS) is a prevalent and high-mortality valve disease. Transcatheter aortic valve replacement (TAVR) is a minimally invasive treatment with outcomes often better than surgery, requiring a multidisciplinary team. This study analyzes outcomes and trends from a high-volume TAVR program in an urban, low-income setting since 2017 to guide future practices. METHODS: Medical records of patients that underwent TAVR were analyzed retrospectively from 2018 to 2023, dividing them into two cohorts to analyze trends in operative details, complications, length of stay (LOS), and readmission/mortality rates. The learning curve (LC) was assessed by operative time. RESULTS: Risk-adjusted cumulative sum (RA-CUSUM) analysis identified the transition point at case 76, dividing the cohort into early LC phase group (n = 76) and post-LC phase group (n = 190). Median operative time decreased from 106 to 80 minutes. After adjustment, operative time was 14% shorter in the post-learning-curve cohort (GMR 0.86, 95% CI 0.77-0.96; p = 0.005). CONCLUSION: Operative time serves as a useful benchmark for institutional maturation. In our program, reaching 76 cases represented a key transition point toward optimized workflows, enhanced efficiency, and improved resource utilization. CI - (c) Indian Association of Cardiovascular-Thoracic Surgeons 2026. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. FAU - Manueli Laos, Emiliano Gabriel AU - Manueli Laos EG AUID- ORCID: 0009-0008-5289-6546 AD - Division of General, Minimally Invasive and Robotic Surgery, Department of Surgery, University of Illinois Chicago, Chicago, IL 60612 USA. ROR: https://ror.org/02mpq6x41. GRID: grid.185648.6. ISNI: 0000 0001 2175 0319 AD - Digestive Disease Institute, Cleveland Clinic, Cleveland, OH 44195 USA. ROR: https://ror.org/03xjacd83. GRID: grid.239578.2. ISNI: 0000 0001 0675 4725 FAU - Fontaine-Nicola, Andres AU - Fontaine-Nicola A AD - Division of Cardiothoracic Surgery, Department of Surgery, University of Illinois Chicago, Chicago, IL 60612 USA. ROR: https://ror.org/02mpq6x41. GRID: grid.185648.6. ISNI: 0000 0001 2175 0319 AD - Department of Surgery, North Shore University Hospital, Zucker School of Medicine at Hofstra/Northwell, Manhasset, NY 11030 USA. ROR: https://ror.org/05m8d2x46. GRID: grid.240382.f. ISNI: 0000 0001 0490 6107 FAU - Zhang, Lily AU - Zhang L AD - Division of General, Minimally Invasive and Robotic Surgery, Department of Surgery, University of Illinois Chicago, Chicago, IL 60612 USA. ROR: https://ror.org/02mpq6x41. GRID: grid.185648.6. ISNI: 0000 0001 2175 0319 FAU - Carlson, Andrew AU - Carlson A AD - Department of Medicine, University of Illinois Chicago, Chicago, IL 60612 USA. ROR: https://ror.org/02mpq6x41. GRID: grid.185648.6. ISNI: 0000 0001 2175 0319 FAU - Masrur, Mario A AU - Masrur MA AD - Division of General, Minimally Invasive and Robotic Surgery, Department of Surgery, University of Illinois Chicago, Chicago, IL 60612 USA. ROR: https://ror.org/02mpq6x41. GRID: grid.185648.6. ISNI: 0000 0001 2175 0319 FAU - Ardati, Amer K AU - Ardati AK AD - Department of Medicine, Division of Cardiology, University of Illinois College of Medicine, Chicago, IL 60612 USA. ROR: https://ror.org/047426m28. GRID: grid.35403.31. ISNI: 0000 0004 1936 9991 FAU - Ibrahim, Khalil AU - Ibrahim K AD - Department of Medicine, Division of Cardiology, University of Illinois College of Medicine, Chicago, IL 60612 USA. ROR: https://ror.org/047426m28. GRID: grid.35403.31. ISNI: 0000 0004 1936 9991 FAU - Shroff, Adhir AU - Shroff A AD - Department of Medicine, Division of Cardiology, University of Illinois College of Medicine, Chicago, IL 60612 USA. ROR: https://ror.org/047426m28. GRID: grid.35403.31. ISNI: 0000 0004 1936 9991 FAU - Abdelhady, Khaled AU - Abdelhady K AD - Division of Cardiothoracic Surgery, Department of Surgery, University of Illinois Chicago, Chicago, IL 60612 USA. ROR: https://ror.org/02mpq6x41. GRID: grid.185648.6. ISNI: 0000 0001 2175 0319 LA - eng PT - Journal Article DEP - 20260804 PL - India TA - Indian J Thorac Cardiovasc Surg JT - Indian journal of thoracic and cardiovascular surgery JID - 8700105 PMC - PMC13714239 OTO - NOTNLM OT - Minimally invasive surgery OT - Single-center experience OT - Transcatheter aortic valve replacement COIS- Conflict of interestThe authors declare no existing conflicts of interest to disclose. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2027/11/01 CRDT- 2026/10/29 05:51 PHST- 2025/11/04 00:00 [received] PHST- 2026/07/03 00:00 [revised] PHST- 2026/07/08 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:51 [entrez] PHST- 2027/11/01 00:00 [pmc-release] AID - 2249 [pii] AID - 10.1007/s12055-026-02249-x [doi] PST - ppublish SO - Indian J Thorac Cardiovasc Surg. 2026 Nov;42(11):1194-1203. doi: 10.1007/s12055-026-02249-x. Epub 2026 Aug 4. PMID- 42953358 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2673-3439 (Electronic) IS - 2673-3439 (Linking) VI - 8 DP - 2026 TI - CRISPR/Cas genome editing strategies to enhance abiotic stress tolerance in sugar crops. PG - 1942811 LID - 10.3389/fgeed.2026.1942811 [doi] LID - 1942811 AB - Sugar crops including sugarcane, sugar beets, and sweet sorghum, are fundamental for global sucrose supply and crucial feedstocks for bioethanol production and industrial bioproducts. However, yield and sucrose accumulation are significantly influenced by abiotic stresses, including drought, salinity/alkalinity, heat, chilling/freezing, and heavy metal toxicity. The polygenic complexity of stress tolerance, particularly in sugarcane with its large, polyploid, and haplotype-diverse genome, presents significant challenges for conventional breeding, rendering it slow and imprecise. This review combines advances in genome-editing technologies, including CRISPR/Cas systems (Cas9 and Cas12a), along with transcriptional techniques (CRISPRa/i), base editors, and prime editors. These tools, combined with the progress in reference genomes, haplotype-resolved assemblies, and pangenomes, have facilitated precise gene discovery, genomic selection, and targeted editing. CRISPR-based approaches offer promising avenues for enhancing key stress-responsive pathways governing oxidative homeostasis, membrane integrity, osmotic regulation, hormone signaling, and ion-homeostasis in sugar crops. Prominent candidate targets include transcription factors (TF), signaling components, raffinose biosynthesis genes implicated in freezing tolerance, regulators of salt and alkaline stress, and determinants of heavy metal tolerance and sequestration. Translational challenges, such as off-target effects, low transformation efficiencies, and editing multiple homologues or haplotypes, are being addressed such as DNA-free ribonucleoprotein (RNP) delivery, high-throughput screening, haplotype-specific target design, and haplotype-aware guide RNA engineering based on unique genetic variation sites across different gene haplotypes. Collectively, the integration of advanced CRISPR toolkits with enhanced genomic information resources and phenotyping capabilities holds significant potential for accelerating the development of precision-bred, multi-stress-resilient sugar crops that sustain yield, quality, and industrial value under abiotic stress conditions. CI - Copyright (c) 2026 Liu, Kumar, Abu-Ellail, Lin, Li, Xu, Arif, Khan, Ismail, Zhao, Sakr, Haq and Lu. FAU - Liu, Hong-Bo AU - Liu HB AD - State Key Laboratory of Tropical Crop Breeding, Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan, China. FAU - Kumar, Tanweer AU - Kumar T AD - State Key Laboratory of Tropical Crop Breeding, Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan, China. AD - Agriculture Research Department Khyber, Sugar Crops Research Institute (SCRI), Mardan, Pakhtunkhwa, Pakistan. FAU - Abu-Ellail, Farrag F B AU - Abu-Ellail FFB AD - State Key Laboratory of Tropical Crop Breeding, Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan, China. AD - Breeding and Genetics Department, Sugar Crops Research Institute (SCRI), Agricultural Research Center (ARC), Giza, Egypt. FAU - Lin, Xiu-Qin AU - Lin XQ AD - State Key Laboratory of Tropical Crop Breeding, Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan, China. FAU - Li, Chun-Jia AU - Li CJ AD - State Key Laboratory of Tropical Crop Breeding, Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan, China. FAU - Xu, Chao-Hua AU - Xu CH AD - State Key Laboratory of Tropical Crop Breeding, Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan, China. FAU - Arif, Muhammad AU - Arif M AD - Agriculture Research Department Khyber, Sugar Crops Research Institute (SCRI), Mardan, Pakhtunkhwa, Pakistan. FAU - Khan, Wajid AU - Khan W AD - Agriculture Research Department Khyber, Sugar Crops Research Institute (SCRI), Mardan, Pakhtunkhwa, Pakistan. FAU - Ismail, Muhammad AU - Ismail M AD - Agriculture Research Department Khyber, Sugar Crops Research Institute (SCRI), Mardan, Pakhtunkhwa, Pakistan. FAU - Zhao, Li-Ping AU - Zhao LP AD - State Key Laboratory of Tropical Crop Breeding, Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan, China. FAU - Sakr, Ebtehag A E AU - Sakr EAE AD - Botany Department, Faculty of Women for Arts, Science, and Education, Ain Shams University, Cairo, Egypt. FAU - Haq, Nur-Ul AU - Haq NU AD - Agriculture Research Department Khyber, Sugar Crops Research Institute (SCRI), Mardan, Pakhtunkhwa, Pakistan. FAU - Lu, Xin AU - Lu X AD - State Key Laboratory of Tropical Crop Breeding, Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan, China. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Genome Ed JT - Frontiers in genome editing JID - 101775540 PMC - PMC13714499 OTO - NOTNLM OT - CRiSPR/Cas OT - abiotic stress OT - genome editing OT - sugar beet OT - sugarcane OT - sweet sorghum COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:50 PHST- 2026/07/20 00:00 [received] PHST- 2026/09/09 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:50 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1942811 [pii] AID - 10.3389/fgeed.2026.1942811 [doi] PST - epublish SO - Front Genome Ed. 2026 Oct 14;8:1942811. doi: 10.3389/fgeed.2026.1942811. eCollection 2026. PMID- 42953354 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1663-9812 (Print) IS - 1663-9812 (Electronic) IS - 1663-9812 (Linking) VI - 17 DP - 2026 TI - Carbon dots derived from traditional Chinese medicines: a novel therapeutic strategy for ocular diseases. PG - 1912653 LID - 10.3389/fphar.2026.1912653 [doi] LID - 1912653 AB - Carbon dots (CDs) represent a novel category of zero-dimensional carbon-based nanomaterials featuring favorable biosafety in vitro and in acute animal studies. They also exhibit distinctive optical properties and diverse pharmacological activities. Synthesized via eco-friendly green routes using traditional Chinese medicines (TCMs) and their extracts, TCM-derived carbon dots (TCM-CDs) display distinct properties compared to conventional nanocarriers. Benefiting from their inherent nanostructures, abundant surface functional groups, and nanozyme-like activity, TCM-CDs can deliver intrinsic therapeutic effects without loading exogenous drugs or relying on residual herbal ingredients. They possess multiple bioactivities, including antioxidant, anti-inflammatory, antibacterial and neuroprotective effects, which make them potential non-invasive therapeutic candidates for refractory ocular disorders. A growing body of studies has validated the promising therapeutic efficacy of TCM-CDs in animal models against autoimmune uveitis, retinal lesions, and optic nerve injuries, highlighting their encouraging preclinical findings that warrant further investigation toward clinical translation. Focusing on the intrinsic therapeutic capacity of TCM-CDs, this review systematically summarizes their core functional mechanisms and research progress in ocular disease intervention, discusses prevailing academic controversies and unresolved research bottlenecks in this field, and proposes prospective developmental directions. This work intends to offer comprehensive theoretical evidence and guidance directions for subsequent mechanistic investigations and clinical translation of TCM-CDs in ophthalmic research. CI - Copyright (c) 2026 Yang, Feng, Zhang, Zhang, Su, Liu, Sa and A. FAU - Yang, Tingyu AU - Yang T AD - Scientific Research Department, Inner Mongolia Fourth Hospital (Chest Hospital), Hohhot, Inner Mongolia, China. FAU - Feng, Zongqi AU - Feng Z AD - Department of Clinical Research and Translational Medicine, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia, China. FAU - Zhang, Xiao AU - Zhang X AD - Department of Clinical Research and Translational Medicine, Inner Mongolia People's Hospital, Hohhot, Inner Mongolia, China. FAU - Zhang, Lihui AU - Zhang L AD - Scientific Research Department, Inner Mongolia Fourth Hospital (Chest Hospital), Hohhot, Inner Mongolia, China. FAU - Su, Yu AU - Su Y AD - Scientific Research Department, Inner Mongolia Fourth Hospital (Chest Hospital), Hohhot, Inner Mongolia, China. FAU - Liu, Ruihua AU - Liu R AD - Scientific Research Department, Inner Mongolia Fourth Hospital (Chest Hospital), Hohhot, Inner Mongolia, China. FAU - Sa, Rina AU - Sa R AD - Scientific Research Department, Inner Mongolia Fourth Hospital (Chest Hospital), Hohhot, Inner Mongolia, China. FAU - A, Lamusi AU - A L AD - Department of Ophthalmology, Inner Mongolia International Hospital of Mongolian Medicine, Hohhot, Inner Mongolia, China. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Pharmacol JT - Frontiers in pharmacology JID - 101548923 PMC - PMC13714506 OTO - NOTNLM OT - TCM-CDs OT - academic controversies OT - carbon dots OT - ocular disease treatment OT - therapeutic mechanisms COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:50 PHST- 2026/06/18 00:00 [received] PHST- 2026/08/29 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:50 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1912653 [pii] AID - 10.3389/fphar.2026.1912653 [doi] PST - epublish SO - Front Pharmacol. 2026 Oct 14;17:1912653. doi: 10.3389/fphar.2026.1912653. eCollection 2026. PMID- 42953336 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2001-0370 (Print) IS - 2001-0370 (Electronic) IS - 2001-0370 (Linking) VI - 35 IP - 1 DP - 2026 TI - In Silico Design and Immunogenicity Studies of Two Multi-Epitope Vaccines against Klebsiella pneumoniae. PG - 0232 LID - 10.34133/csbj.0232 [doi] LID - 0232 AB - Klebsiella pneumoniae represents an important clinical problem worldwide, causing infections in healthy and immunocompromised individuals that are often resistant to multiple antibiotics. The diversity of potential antigens encoded by K. pneumoniae makes the development of a protein-based vaccine that combines different antigens a promising strategy to achieve high vaccine coverage. This study employs 8 highly conserved virulence factors encoded by K. pneumoniae to construct multi-epitope vaccines. The target proteins used here are the fimbrial subunit MrkA, the outer membrane proteins and porins OmpA, OmpW, LamB, and PhoE, and the siderophore receptors FepA, FhuA, and Fiu. Two different immunoinformatics strategies (BEPI and VAXI) were followed to select one B cell epitope per protein for vaccine construct design. Whereas the BEPI-strategy prioritizes the B cell epitope prediction score, the VAXI-strategy gives importance to B cell epitope antigenicity score. The validated multi-epitope sequences were fused to the immunostimulatory PADRE peptide. BEPI- and VAXI-vaccine proteins were predicted to be stable and easily expressed in Escherichia coli and Homo sapiens. Moreover, both met the safety thresholds of non-allergenicity, non-toxicity, and non-human homology. Specifically, the VAXI-vaccine protein scored higher for solubility and antigenicity and was predicted to induce improved humoral and cellular responses. The predicted secondary structures of both vaccines consisted of >70% random coils, >15% alpha-helices, and ~10% beta-strands, and their predicted 3-dimensional models indicated that most regions were exposed. Finally, >99.9% of global population coverage was predicted for both multi-epitope vaccines. We thus present 2 multi-epitope vaccines against K. pneumoniae virulence factors with promising physicochemical profiles regarding production and potential safety for preclinical testing and eventual use in humans. CI - Copyright (c) 2026 Marta Vicente-Pazos et al. FAU - Vicente-Pazos, Marta AU - Vicente-Pazos M AD - Intrahospital Infections Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III (ISCIII), Madrid, Spain. AD - Doctoral Program in Biomedical Sciences and Public Health, IMIENS, Universidad Nacional de Educacion a Distancia (UNED), Madrid, Spain. AD - Microbiology of Intestinal Diseases, Biology Department, Universitat de Girona, Girona, Spain. FAU - Lopez-Siles, Mireia AU - Lopez-Siles M AUID- ORCID: 0000-0002-5805-8446 AD - Microbiology of Intestinal Diseases, Biology Department, Universitat de Girona, Girona, Spain. FAU - McConnell, Michael J AU - McConnell MJ AD - Intrahospital Infections Laboratory, National Centre for Microbiology, Instituto de Salud Carlos III (ISCIII), Madrid, Spain. AD - Department of Biological Sciences, University of Notre Dame, Notre Dame, IN, USA. AD - Eck Institute for Global Health, Notre Dame, IN, USA. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Comput Struct Biotechnol J JT - Computational and structural biotechnology journal JID - 101585369 PMC - PMC13714322 COIS- Competing interests: M.V.-P. and M.L.-S. declare that they have no competing interests. M.J.M. is founder and shareholder of the biotechnology spin-off company Vaxdyn S.L. Vaxdyn had no role in the elaboration of this manuscript. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/28 CRDT- 2026/10/29 05:50 PHST- 2025/10/03 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:50 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 0232 [pii] AID - 10.34133/csbj.0232 [doi] PST - epublish SO - Comput Struct Biotechnol J. 2026 Oct 28;35(1):0232. doi: 10.34133/csbj.0232. eCollection 2026. PMID- 42953333 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 17 DP - 2026 TI - Corticosteroid response and biopsy decisions in tumefactive demyelination and primary central nervous system lymphoma. PG - 1988759 LID - 10.3389/fimmu.2026.1988759 [doi] LID - 1988759 AB - Distinguishing primary central nervous system lymphoma (PCNSL) from tumefactive demyelination becomes especially difficult after corticosteroid treatment. Both conditions may improve clinically and radiologically, while corticosteroids can reduce lymphoma cellularity, alter enhancement, and change the tissue available for sampling. The biopsy literature reflects this time dependence. Meta-analysis shows a higher average risk of nondiagnostic tissue after prebiopsy exposure, yet contemporary cohorts report high diagnostic yields in selected patients when enhancement persists and current imaging guides prompt sampling. We organize the evidence around four time-linked clinical questions. Before corticosteroids (T0), clinicians establish the competing probabilities and preserve baseline anatomy. After treatment (T1), they measure the anatomical and clinical transformation. Before biopsy (T2), current imaging should determine whether a representative target remains visible, safely accessible, and sufficiently sampleable. A persistent useful target supports prompt, imaging-updated biopsy; marked regression calls for planned short-interval imaging to recover a representative target. Cerebrospinal fluid biomarkers can modify probability, although single-marker negativity is weak and composite results remain prevalence- and platform-dependent. After biopsy (T3), pathology must fit the sampled site and subsequent course. A major verified inconsistency should reopen imaging-pathology review and prompt repeat biopsy when a useful target is available. This approach treats corticosteroid response as a measurable change in diagnostic substrate and links each decision to the evidence available at that time. CI - Copyright (c) 2026 Wang and Guo. FAU - Wang, Xu AU - Wang X AD - Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. FAU - Guo, Shougang AU - Guo S AD - Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 0 (Adrenal Cortex Hormones) SB - IM MH - Humans MH - *Demyelinating Diseases/drug therapy/diagnosis/pathology MH - *Central Nervous System Neoplasms/drug therapy/diagnosis/pathology MH - *Adrenal Cortex Hormones/therapeutic use MH - Biopsy MH - *Lymphoma/drug therapy/diagnosis/pathology MH - Diagnosis, Differential MH - Magnetic Resonance Imaging PMC - PMC13715385 OTO - NOTNLM OT - brain biopsy OT - corticosteroids OT - magnetic resonance imaging OT - neuroimmunology OT - primary central nervous system lymphoma OT - targetability OT - tumefactive demyelination COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:49 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:49 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fimmu.2026.1988759 [doi] PST - epublish SO - Front Immunol. 2026 Oct 14;17:1988759. doi: 10.3389/fimmu.2026.1988759. eCollection 2026. PMID- 42953329 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2950-1954 (Electronic) IS - 2950-1954 (Linking) VI - 14 DP - 2026 Dec TI - Clinical utility of circulating tumor cell PD-L1 expression for predicting immunotherapeutic response and prognosis in esophageal cancer. PG - 100681 LID - 10.1016/j.jlb.2026.100681 [doi] LID - 100681 AB - BACKGROUND: Immune checkpoint inhibitors (ICIs) have improved outcomes in esophageal cancer, yet reliable biomarkers remain lacking. Tissue PD-L1 testing is limited by intratumoral heterogeneity and invasiveness, whereas circulating tumor cells (CTCs) offer a non-invasive alternative. This study evaluated CTC PD-L1 expression as a predictive and prognostic biomarker in patients with advanced esophageal cancer receiving chemo-immunotherapy. METHODS: A total of 93 patients with stage III-IV esophageal cancer were enrolled. The primary biomarker analysis was restricted to 59 patients who received chemo-immunotherapy, while 34 patients treated with chemotherapy alone were analyzed separately. Baseline CTCs were isolated and PD-L1 expression assessed, alongside tissue PD-L1 (tPD-L1) by immunohistochemistry. Associations with objective response rate (ORR), progression-free survival (PFS), and overall survival (OS) were evaluated using ROC curves and univariate and multivariable Cox regression. RESULTS: CTC positivity correlated significantly with advanced stage, metastasis (p = 0.0411), and larger tumor size (p = 0.0454). In the chemo-immunotherapy cohort, CTC PD-L1 positivity was associated with a higher ORR (75.0% vs. 29.6%, p < 0.001) and showed moderate predictive ability (AUC = 0.7049, p = 0.0037). CTC PD-L1 positivity was significantly associated with OS (HR = 0.254, p = 0.002), but not significantly with PFS in univariate analysis (HR = 0.546, p = 0.042; Kaplan-Meier p = 0.061). Conversely, tPD-L1 status was not significantly associated with PFS or OS (both p > 0.05). In multivariable analysis adjusting for age, sex, histological subtype, stage, metastasis, and treatment modality, CTC PD-L1 remained an independent prognostic factor for PFS (HR = 0.488, p = 0.039) and OS (HR = 0.244, p = 0.002). CONCLUSIONS: Baseline CTC PD-L1 expression was associated with favorable response and OS in patients receiving chemo-immunotherapy, and may complement tissue testing. These exploratory findings require prospective validation in larger cohorts before clinical use. CI - (c) 2026 The Authors. Published by Elsevier B.V. on behalf of The International Society of Liquid Biopsy. FAU - Li, Jing AU - Li J AD - Department of Oncology, The First Affiliated Hospital of Zhengzhou University, longhu zhonghuan road, No.1, Zhengzhou, Henan, 450000, P. R. China. FAU - Li, Yueze AU - Li Y AD - Department of Oncology, The First Affiliated Hospital of Zhengzhou University, longhu zhonghuan road, No.1, Zhengzhou, Henan, 450000, P. R. China. FAU - Chen, Lin AU - Chen L AD - Zhuhai Sanmed Biotech Ltd., 266 Tongchang Road, Zhuhai, Guangdong, 519060, P. R. China. FAU - Yang, Zhonglin AU - Yang Z AD - Zhuhai Sanmed Biotech Ltd., 266 Tongchang Road, Zhuhai, Guangdong, 519060, P. R. China. FAU - Lu, Yue AU - Lu Y AD - Zhuhai Sanmed Biotech Ltd., 266 Tongchang Road, Zhuhai, Guangdong, 519060, P. R. China. FAU - Tang, Dongjiang AU - Tang D AD - Zhuhai Sanmed Biotech Ltd., 266 Tongchang Road, Zhuhai, Guangdong, 519060, P. R. China. FAU - Qin, Yanru AU - Qin Y AD - Department of Oncology, The First Affiliated Hospital of Zhengzhou University, longhu zhonghuan road, No.1, Zhengzhou, Henan, 450000, P. R. China. LA - eng PT - Journal Article DEP - 20261021 PL - United States TA - J Liq Biopsy JT - The journal of liquid biopsy JID - 9918957761506676 PMC - PMC13715408 OTO - NOTNLM OT - Circulating tumor cells OT - Esophageal cancer OT - Immunotherapy OT - Liquid biopsy OT - PD-L1 OT - Predictive biomarker COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/21 CRDT- 2026/10/29 05:49 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:49 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - S2950-1954(26)00227-4 [pii] AID - 100681 [pii] AID - 10.1016/j.jlb.2026.100681 [doi] PST - epublish SO - J Liq Biopsy. 2026 Oct 21;14:100681. doi: 10.1016/j.jlb.2026.100681. eCollection 2026 Dec. PMID- 42953321 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2590-1567 (Electronic) IS - 2590-1567 (Linking) VI - 12 DP - 2026 Dec TI - Anti-PD-L1 antibody-coated metal-organic framework for targeted PTPN2 Inhibitor delivery and enhanced immunotherapy of head and neck squamous cell carcinoma via cGAS-STING activation. PG - 100694 LID - 10.1016/j.ijpx.2026.100694 [doi] LID - 100694 AB - Head and neck squamous cell carcinoma (HNSCC) is a malignancy with limited responses to immune checkpoint blockade, due to insufficient tumor immunogenicity and immunosuppressive signaling. Protein tyrosine phosphatase non-receptor type 2 (PTPN2) has emerged to negatively regulate antitumor immune responses. However, effective tumor-targeted delivery of PTPN2 inhibitors remains a major challenge. Herein, we developed an anti-PD-L1 antibody-functionalized Mn metal-organic framework nanoplatform (Pi@MOF@aP). The manganese-based MOF core efficiently encapsulated the PTPN2 inhibitor and enabled pH-responsive release under the acidic tumor microenvironment. Surface modification with anti-PD-L1 antibodies enhanced tumor targeting, promoted selective cellular uptake, and provided additional immune checkpoint blockade. Pi@MOF@aP exhibited potent cytotoxicity against HNSCC cells while maintaining favorable compatibility with normal oral keratinocytes. Mechanistically, Pi@MOF@aP-mediated PTPN2 inhibition activated the cGAS-STING pathway, increased cytokine production, and enhanced antitumor immune responses. In HNSCC mouse models, Pi@MOF@aP achieved efficient tumor accumulation, significantly inhibited tumor growth, prolonged survival, and promoted CD4(+) and CD8(+) T cells; infiltration with no obvious toxicity. Therefore, this study presents a multifunctional nanotherapeutic platform that integrates targeted PTPN2 inhibition, PD-L1 blockade, and cGAS-STING activation, providing a promising strategy for improving immunotherapy efficacy in HNSCC. CI - (c) 2026 The Authors. FAU - Zhu, Zhanpeng AU - Zhu Z AD - Department of Neurovascular Surgery, First Hospital of Jilin University, Changchun, Jilin, 130021, China. FAU - Wang, Rui AU - Wang R AD - Department of Thoracic Surgery II, Department of Lung Transplantation, Organ Transplantation Center, First Hospital of Jilin University, Changchun, Jilin, 130021, China. FAU - Li, Wei AU - Li W AD - Department of Clinical Laboratory, China-Japan Union Hospital of Jilin University, Changchun, Jilin, 130033, China. FAU - Cui, Yu AU - Cui Y AD - Department of Otolaryngology-Head and Neck Surgery, First Hospital of Jilin University, Changchun, Jilin, 130021, China. LA - eng PT - Journal Article DEP - 20261020 PL - Netherlands TA - Int J Pharm X JT - International journal of pharmaceutics: X JID - 101753452 PMC - PMC13715415 OTO - NOTNLM OT - Anti-PD-L1 antibody OT - Head and neck squamous cell carcinoma OT - Metal-organic framework OT - PTPN2 inhibitor OT - cGAS-STING signaling COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/20 CRDT- 2026/10/29 05:49 PHST- 2026/08/25 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:49 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - S2590-1567(26)00214-8 [pii] AID - 100694 [pii] AID - 10.1016/j.ijpx.2026.100694 [doi] PST - epublish SO - Int J Pharm X. 2026 Oct 20;12:100694. doi: 10.1016/j.ijpx.2026.100694. eCollection 2026 Dec. PMID- 42953320 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2054-5703 (Print) IS - 2054-5703 (Electronic) IS - 2054-5703 (Linking) VI - 13 IP - 10 DP - 2026 Oct 7 TI - A unique ecological phenomenon quantified by hydroacoustics: the deep-sea eel spawning aggregation at Patience Seamount. PG - 260397 LID - 10.1098/rsos.260397 [doi] LID - 260397 AB - Patience Seamount, within Australia's Huon Marine Park, hosts persistent deep-sea fish aggregations dominated by basketwork eels (Diastobranchus capensis) and orange roughy (Hoplostethus atlanticus). Using integrated hydroacoustic surveys, deep towed camera imagery and physical sampling from 2023 to 2025, we quantified the spatial and temporal dynamics of these assemblages and produced species-specific biomass estimates. Acoustic backscatter attribution followed a two-phase approach: (i) camera-derived species distributions to inform spatial apportioning of backscatter and (ii) conducting biomass-constrained sensitivity analyses applying conservative bounds on orange roughy biomass (200-850 t) informed by historical assessments. We found a large, seasonally variable basketwork eel aggregation that increased markedly from March to May, when biomass reached 3547-3557 t (approx. 2.8 million individuals), likely corresponding to spawning. Distinct habitat preferences-with basketwork eels on deeper, flatter terrain and orange roughy on steep summit slopes-improved spatial separation of backscatter in this mixed-species environment. Without biomass constraints, orange roughy estimates became implausible relative to regional stock assessments, underscoring the need for species discrimination in deep-sea acoustic analyses. Patience Seamount's protection and recovery from historical fishing provide a rare opportunity for long-term study of deep-sea ecological processes. Our methods offer a scalable, non-invasive framework for monitoring deep-sea aggregations in marine protected areas. CI - (c) 2026 The Authors. FAU - Scoulding, Ben AU - Scoulding B AUID- ORCID: 0000-0002-9358-736X AD - Environment, Commonwealth Scientific and Industrial Research Organization, Hobart, Tasmania, Australia. FAU - Gastauer, Sven AU - Gastauer S AD - Thunen Institute of Sea Fisheries, Bremerhaven, Germany. FAU - Devine, Carlie AU - Devine C AD - Environment, Commonwealth Scientific and Industrial Research Organization, Hobart, Tasmania, Australia. FAU - Althaus, Franziska AU - Althaus F AD - Environment, Commonwealth Scientific and Industrial Research Organization, Hobart, Tasmania, Australia. FAU - Hobday, Alistair AU - Hobday A AD - Environment, Commonwealth Scientific and Industrial Research Organization, Hobart, Tasmania, Australia. FAU - Farley, Jessica AU - Farley J AD - Environment, Commonwealth Scientific and Industrial Research Organization, Hobart, Tasmania, Australia. FAU - Deagle, Bruce AU - Deagle B AD - NCMI, Commonwealth Scientific and Industrial Research Organization, Hobart, Tasmania, Australia. FAU - Pogonoski, John AU - Pogonoski J AD - NCMI, Commonwealth Scientific and Industrial Research Organization, Hobart, Tasmania, Australia. FAU - Gorton, Rebecca AU - Gorton R AD - Environment, Commonwealth Scientific and Industrial Research Organization, Hobart, Tasmania, Australia. FAU - Downie, Ryan AU - Downie R AD - Environment, Commonwealth Scientific and Industrial Research Organization, Hobart, Tasmania, Australia. FAU - Williams, Alan AU - Williams A AD - Environment, Commonwealth Scientific and Industrial Research Organization, Hobart, Tasmania, Australia. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - R Soc Open Sci JT - Royal Society open science JID - 101647528 PMC - PMC13714380 OTO - NOTNLM OT - Australian marine parks OT - Diastobranchus capensis OT - Hoplostethus atlanticus OT - basketwork eel OT - conservation OT - monitoring OT - optics OT - orange roughy COIS- We declare we have no competing interests. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/28 CRDT- 2026/10/29 05:48 PHST- 2026/02/24 00:00 [received] PHST- 2026/09/10 00:00 [revised] PHST- 2026/09/10 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:48 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - rsos260397 [pii] AID - 10.1098/rsos.260397 [doi] PST - epublish SO - R Soc Open Sci. 2026 Oct 28;13(10):260397. doi: 10.1098/rsos.260397. eCollection 2026 Oct 7. PMID- 42953300 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 0970-9134 (Print) IS - 0973-7723 (Electronic) IS - 0970-9134 (Linking) VI - 42 IP - 11 DP - 2026 Nov TI - Testosterone replacement therapy and aneurysm biology: current evidence and clinical implications. PG - 1216-1224 LID - 10.1007/s12055-026-02250-4 [doi] AB - BACKGROUND: Testosterone replacement therapy (TRT) is increasingly used to treat male hypogonadism, but its role in aneurysm biology remains unclear. Because aneurysm formation is driven by inflammation, extracellular matrix degradation, and vascular remodeling, testosterone may influence disease progression through androgen receptor-mediated pathways. METHODS: A narrative literature review was conducted using PubMed/MEDLINE, EMBASE, and Google Scholar (August-September 2025). Studies examining testosterone, TRT, vascular inflammation, and aneurysm-related outcomes, including experimental, preclinical, and clinical studies, were included. EVIDENCE SYNTHESIS: Available evidence is mostly mechanistic and preclinical. Animal studies indicate vascular bed-specific effects: physiological androgen signaling seems protective in abdominal aortic aneurysm models by reducing macrophage infiltration and inflammatory cytokine signaling, whereas exogenous or supraphysiologic testosterone may exacerbate oxidative stress and vascular injury in intracranial aneurysm models. Human data remain indirect, with no prospective studies specifically assessing aneurysm formation, growth, or rupture in TRT users. Large randomized trials and meta-analyses indicate cardiovascular safety comparable to placebo in appropriately selected patients, though higher rates of atrial fibrillation, pulmonary embolism, and erythrocytosis have been reported. CONCLUSION: Testosterone has context-, dose-, and vascular bed-specific effects on aneurysm biology. Until clinical studies on aneurysm outcomes are available, TRT should be individualized, dosed to meet physiological needs, and closely monitored for cardiovascular and vascular health. No evidence-based recommendation can currently be made to initiate, withhold, or modify TRT solely on the basis of aneurysm status. Multidisciplinary discussion and continued standard aneurysm surveillance are advisable for hypogonadal patients with known aneurysmal disease. CI - (c) The Author(s) 2026. FAU - de Oliveira Manduca Palmiero, Helbert AU - de Oliveira Manduca Palmiero H AUID- ORCID: 0000-0001-5951-8371 AD - Division of Neurosurgery, University of Sao Paulo Medical School, Dr. Eneas de Carvalho Aguiar Ave., , Sao Paulo, Room5083, 25505403-000 Brazil. ROR: https://ror.org/036rp1748. GRID: grid.11899.38. ISNI: 0000 0004 1937 0722 FAU - Gadelha Figueiredo, Eberval AU - Gadelha Figueiredo E AD - Division of Neurosurgery, University of Sao Paulo Medical School, Dr. Eneas de Carvalho Aguiar Ave., , Sao Paulo, Room5083, 25505403-000 Brazil. ROR: https://ror.org/036rp1748. GRID: grid.11899.38. ISNI: 0000 0004 1937 0722 LA - eng PT - Journal Article PT - Review DEP - 20260730 PL - India TA - Indian J Thorac Cardiovasc Surg JT - Indian journal of thoracic and cardiovascular surgery JID - 8700105 PMC - PMC13713931 OTO - NOTNLM OT - Abdominal Aortic Aneurysm OT - Aneurysm Formation OT - Inflammation OT - Intracranial Aneurysm OT - Testosterone Replacement Therapy OT - Vascular Remodeling COIS- Competing interestsThe authors declare no conflicts of interest concerning the materials or methods used in this study or the findings specified in this paper. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/07/30 CRDT- 2026/10/29 05:48 PHST- 2026/02/13 00:00 [received] PHST- 2026/07/08 00:00 [revised] PHST- 2026/07/08 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:48 [entrez] PHST- 2026/07/30 00:00 [pmc-release] AID - 2250 [pii] AID - 10.1007/s12055-026-02250-4 [doi] PST - ppublish SO - Indian J Thorac Cardiovasc Surg. 2026 Nov;42(11):1216-1224. doi: 10.1007/s12055-026-02250-4. Epub 2026 Jul 30. PMID- 42953292 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-2295 (Electronic) IS - 1664-2295 (Linking) VI - 17 DP - 2026 TI - FLAIR vascular hyperintensities in acute ischemic stroke: from collateral-related imaging findings to a candidate dynamic hemodynamic imaging phenotype. PG - 1960930 LID - 10.3389/fneur.2026.1960930 [doi] LID - 1960930 AB - With advances in reperfusion therapies for acute large vessel occlusion (LVO), stroke imaging evaluation has progressively shifted from time-based treatment selection toward comprehensive assessment of tissue status and hemodynamic adaptation. However, current imaging approaches primarily characterize vascular patency, perfusion status, and tissue injury, while remaining limited in their ability to directly capture the dynamic regulatory processes of cerebral circulation after arterial occlusion. In patients with LVO, ischemic tissue outcome is determined not only by the occlusion site and time to reperfusion but also by collateral circulation capacity, cerebral hemodynamic status, and tissue tolerance to ischemia. Therefore, imaging biomarkers capable of reflecting cerebral circulatory adaptation under ischemic conditions are of substantial research interest. FLAIR vascular hyperintensities (FVHs) are MRI findings characterized by linear or serpentine hyperintense signals along the cortical surface, within cortical sulci, or along the Sylvian fissure on fluid-attenuated inversion recovery (FLAIR) sequences. They are generally associated with reduced distal blood flow velocity, prolonged blood transit time, and hemodynamic alterations mediated by leptomeningeal collateral pathways. In recent years, numerous studies have investigated the role of FVHs in the diagnosis of acute ischemic stroke, evaluation of reperfusion therapy, and prediction of clinical outcomes. However, the clinical significance of FVHs remains incompletely understood. Some studies have suggested that FVHs are associated with favorable collateral status and smaller infarct volumes, whereas others have demonstrated that FVHs may also occur in patients with more pronounced hemodynamic impairment. These discrepancies suggest that FVHs may not simply represent favorable collateral circulation or ischemic severity, but may instead reflect the dynamic hemodynamic response of the cerebral circulation to reduced perfusion following arterial occlusion. From the perspective of precision stroke imaging, this review systematically summarizes the mechanisms underlying FVH formation, their biological significance, and clinical implications. Particular emphasis is placed on the relationships between FVHs and collateral circulation, FVHs-DWI mismatch, ischemic tissue status, and potential benefit from reperfusion therapy. Furthermore, current challenges related to FVH standardization and clinical translation are discussed, with particular emphasis on their potential role as a candidate dynamic hemodynamic imaging phenotype reflecting physiological hemodynamic adaptation. CI - Copyright (c) 2026 Qin, Wang and Han. FAU - Qin, Mingqian AU - Qin M AD - Affiliated Hospital of Shandong Second Medical University, Weifang, China. FAU - Wang, Lei AU - Wang L AD - Affiliated Hospital of Shandong Second Medical University, Weifang, China. FAU - Han, Guosheng AU - Han G AD - Affiliated Hospital of Shandong Second Medical University, Weifang, China. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Neurol JT - Frontiers in neurology JID - 101546899 SB - IM MH - Humans MH - *Ischemic Stroke/diagnostic imaging/physiopathology MH - *Collateral Circulation/physiology MH - *Hemodynamics/physiology MH - *Cerebrovascular Circulation/physiology MH - *Magnetic Resonance Imaging/methods MH - Animals MH - *Brain Ischemia/diagnostic imaging/physiopathology MH - Phenotype PMC - PMC13714180 OTO - NOTNLM OT - FLAIR vascular hyperintensities OT - FVHs OT - FVHs-DWI mismatch OT - acute ischemic stroke OT - collateral circulation OT - hemodynamic adaptation OT - large vessel occlusion (LVO) OT - reperfusion therapy COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:47 PHST- 2026/08/07 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:47 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fneur.2026.1960930 [doi] PST - epublish SO - Front Neurol. 2026 Oct 14;17:1960930. doi: 10.3389/fneur.2026.1960930. eCollection 2026. PMID- 42953287 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2832-3637 (Electronic) IS - 2832-3637 (Linking) VI - 4 IP - 10 DP - 2026 Oct 26 TI - Recent Advances in Near-Infrared Probes for Monitoring Drug Treatment Responses: Toward Efficacy Evaluation and High-Throughput Screening. PG - 2159-2179 LID - 10.1021/cbmi.6c00055 [doi] AB - Accurate drug efficacy evaluation and efficient potential drug screening are cornerstones of translational pharmacology, as they can directly underpin the optimization of therapeutic regimens, acceleration of drug development pipelines, and improvement of patient clinical outcomes. However, conventional analytical approaches for these tasks often suffer from inherent limitations, including invasiveness, low sensitivity to subtle molecular changes, inability to achieve real-time in vivo monitoring of therapeutic dynamics, and poor compatibility with physiologically relevant disease models. In response to these unmet needs, near-infrared (NIR) fluorescent probes have emerged as transformative tools, leveraging a suite of unique advantages, such as noninvasiveness, ultrahigh sensitivity, excellent target specificity, operational simplicity, deep tissue penetration, and cost-effectiveness, to enable precise, spatiotemporal tracking of drug actions in both preclinical and early clinical settings. This review provides a comprehensive, up-to-date overview of recent advances in the rational design and translational applications of NIR probes for drug efficacy assessment and high-throughput screening (HTS). Specifically, NIR probes can systematically detect drug-induced biochemical and microenvironmental alterations and, when are integrated into automated HTS platforms, significantly enhancing screening sensitivity, throughput, and physiological relevance. Finally, we delineate key challenges impeding the widespread clinical translation of NIR probe technology and outline future research directions. Progress in therapeutic areas holds profound potential to refine personalized drug treatment strategies, accelerate late-stage clinical trial validation, and drive paradigm-shifting innovations across the global pharmaceutical industry. CI - (c) 2026 The Authors. Co-published by Nanjing University and American Chemical Society. FAU - Li, Xinle AU - Li X AD - School of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang 311121, China. RINGGOLD: 26494 FAU - Hou, Shaobo AU - Hou S AD - School of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang 311121, China. RINGGOLD: 26494 FAU - He, Zhenghui AU - He Z AD - School of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang 311121, China. RINGGOLD: 26494 FAU - Sun, Yi AU - Sun Y AD - School of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang 311121, China. RINGGOLD: 26494 FAU - Sun, Yingxin AU - Sun Y AD - School of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang 311121, China. RINGGOLD: 26494 FAU - Yao, Yalun AU - Yao Y AD - School of Civil and Architectural Engineering, Jiangxi University of Water Resources and Electric Power, Nanchang 330099, China. FAU - Long, Zi AU - Long Z AD - School of Civil and Architectural Engineering, Jiangxi University of Water Resources and Electric Power, Nanchang 330099, China. FAU - Duan, Chong AU - Duan C AUID- ORCID: 0000-0003-1033-2415 AD - School of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang 311121, China. RINGGOLD: 26494 FAU - Zhou, Jian-Liang AU - Zhou JL AUID- ORCID: 0000-0002-4716-7807 AD - School of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang 311121, China. RINGGOLD: 26494 LA - eng PT - Journal Article PT - Review DEP - 20260518 PL - United States TA - Chem Biomed Imaging JT - Chemical & biomedical imaging JID - 9918574388106676 PMC - PMC13715351 OTO - NOTNLM OT - Biomarkers OT - Drug efficacy OT - High-throughput screening OT - Near-infrared probes OT - Therapeutic monitoring EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/05/18 CRDT- 2026/10/29 05:47 PHST- 2026/02/25 00:00 [received] PHST- 2026/05/08 00:00 [revised] PHST- 2026/05/09 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:47 [entrez] PHST- 2026/05/18 00:00 [pmc-release] AID - 10.1021/cbmi.6c00055 [doi] PST - epublish SO - Chem Biomed Imaging. 2026 May 18;4(10):2159-2179. doi: 10.1021/cbmi.6c00055. eCollection 2026 Oct 26. PMID- 42953286 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 0970-9134 (Print) IS - 0973-7723 (Electronic) IS - 0970-9134 (Linking) VI - 42 IP - 11 DP - 2026 Nov TI - Complete resection and left ventricular reconstruction for giant cardiac fibroma in a 2-year-old child. PG - 1238-1244 LID - 10.1007/s12055-026-02242-4 [doi] AB - Complete surgical excision is the preferred treatment for selected cardiac fibromas, although reconstruction depends on tumor location and myocardial involvement. Cardiac fibromas are the second most common primary cardiac tumor in children. They most commonly arise from the ventricular free wall or interventricular septum. Several treatment strategies have been reported, including surgical resection, single-ventricle palliation, heart transplantation, and conservative management for primary benign cardiac tumors. Recent evidence suggests that surgical resection can be curative in selected patients, eliminating the long-term dependence on antiarrhythmic drugs and avoiding the necessity for implantable defibrillators. Although this approach is now considered standard practice in some institutions-even for extensive fibromas-its adoption remains limited. We report the case of a 2-year-old child with a giant cardiac fibroma treated with complete tumor excision using a combined endocardial patch and two-layered ventricular reconstruction. The early postoperative course was uneventful, with preserved ventricular function at short-term follow-up. CI - (c) Indian Association of Cardiovascular-Thoracic Surgeons 2026. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. FAU - Garcia-Rojas, Frank AU - Garcia-Rojas F AUID- ORCID: 0000-0002-2361-5644 AD - National University of San Marcos, Lima, Peru. ROR: https://ror.org/006vs7897. GRID: grid.10800.39. ISNI: 0000 0001 2107 4576 AD - Cardiovascular Surgery Department, National University of San Marcos, Lima, Peru. ROR: https://ror.org/006vs7897. GRID: grid.10800.39. ISNI: 0000 0001 2107 4576 AD - National Cardiovascular Institute "Carlos A. Peschiera" (INCOR), Lima, Peru. FAU - Aguilar, Cristian AU - Aguilar C AD - Laboratory of Pathology, National University of San Marcos, Lima, Peru. ROR: https://ror.org/006vs7897. GRID: grid.10800.39. ISNI: 0000 0001 2107 4576 AD - National Cardiovascular Institute "Carlos A. Peschiera" (INCOR), Lima, Peru. FAU - Cornejo-Acevedo, Jose AU - Cornejo-Acevedo J AD - Cardio Pediatric Department, National University of San Marcos, Lima, Peru. ROR: https://ror.org/006vs7897. GRID: grid.10800.39. ISNI: 0000 0001 2107 4576 AD - National Cardiovascular Institute "Carlos A. Peschiera" (INCOR), Lima, Peru. FAU - Palma-Ortecho, Luis AU - Palma-Ortecho L AD - National University of San Marcos, Lima, Peru. ROR: https://ror.org/006vs7897. GRID: grid.10800.39. ISNI: 0000 0001 2107 4576 AD - Cardiovascular Surgery Department, National University of San Marcos, Lima, Peru. ROR: https://ror.org/006vs7897. GRID: grid.10800.39. ISNI: 0000 0001 2107 4576 AD - National Cardiovascular Institute "Carlos A. Peschiera" (INCOR), Lima, Peru. FAU - Chavarri-Velarde, Fernando AU - Chavarri-Velarde F AD - National University of San Marcos, Lima, Peru. ROR: https://ror.org/006vs7897. GRID: grid.10800.39. ISNI: 0000 0001 2107 4576 AD - Cardiovascular Surgery Department, National University of San Marcos, Lima, Peru. ROR: https://ror.org/006vs7897. GRID: grid.10800.39. ISNI: 0000 0001 2107 4576 AD - National Cardiovascular Institute "Carlos A. Peschiera" (INCOR), Lima, Peru. LA - eng PT - Case Reports PT - Journal Article DEP - 20260730 PL - India TA - Indian J Thorac Cardiovasc Surg JT - Indian journal of thoracic and cardiovascular surgery JID - 8700105 PMC - PMC13713965 OTO - NOTNLM OT - Cardiac tumors; Myocardial remodeling/reshaping/ventriculectomy; Ventricular fibroma COIS- Competing interestsThe authors declare no competing interests. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2027/11/01 CRDT- 2026/10/29 05:47 PHST- 2025/12/30 00:00 [received] PHST- 2026/06/29 00:00 [revised] PHST- 2026/06/29 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:47 [entrez] PHST- 2027/11/01 00:00 [pmc-release] AID - 2242 [pii] AID - 10.1007/s12055-026-02242-4 [doi] PST - ppublish SO - Indian J Thorac Cardiovasc Surg. 2026 Nov;42(11):1238-1244. doi: 10.1007/s12055-026-02242-4. Epub 2026 Jul 30. PMID- 42953281 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2168-8184 (Print) IS - 2168-8184 (Electronic) IS - 2168-8184 (Linking) VI - 18 IP - 9 DP - 2026 Sep TI - Laparoscopic and Robot-Assisted Surgery for Gestational Trophoblastic Disease: A Scoping Review of Patient Selection and Intraoperative Precautions. PG - e117071 LID - 10.7759/cureus.117071 [doi] LID - e117071 AB - Guidelines for gestational trophoblastic disease (GTD) state when hysterectomy is indicated, but say little about the operative route or what should be recorded during the operation. We conducted a Joanna Briggs Institute (JBI) scoping review, reported to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR), of laparoscopic and robot-assisted operations that resected or biopsied GTD tissue to establish which patients undergo these operations and what intraoperative precautions the reports contain. PubMed (August 18, 2026; 449 records) and Ichushi-Web (August 20, 2026; 329 records) were searched without language or date restriction, and backward citation chasing added two reports. The review maps what is reported; it estimates neither safety nor effectiveness, and no meta-analysis was performed. In total, 64 original reports describing 67 operations entered the main analysis, alongside 24 Ichushi-Web conference records as a supplementary layer; 54 were case reports, and the literature spans 1995 to 2026. GTD had been diagnosed before 38 of the 67 operations; before the other 29, it had not. The details needed to study intraperitoneal containment were rarely described: use or non-use of a uterine manipulator in five of 36 evaluable reports of operations on a retained uterus, morcellation in three of 36, a retrieval bag in two of 50, peritoneal washing cytology in six of 53, and pneumoperitoneum pressure in none of 52. A mechanical containment token was present in 17 of 57 evaluable reports, but of the 16 that could also be assessed for wording, 12 never used the words spillage, seeding, dissemination, or port-site. Port-site events were charted as an outcome and were not reported in 43 of the 49 reports in which that field was evaluable. Laparoscopic and robot-assisted surgery for GTD has been reported since 1995, mainly as single cases and in a preoperatively mixed population, and the intraoperative detail that would allow containment to be studied is usually absent. The gap this review identifies is one of reporting rather than of technique. CI - Copyright (c) 2026, Gou et al. FAU - Gou, Rei AU - Gou R AD - Department of Obstetrics and Gynecology, Yamanashi Red Cross Hospital, Kawaguchiko, JPN. FAU - Yuda, Makiko AU - Yuda M AD - Department of Obstetrics and Gynecology, Yamanashi Red Cross Hospital, Kawaguchiko, JPN. FAU - Fukasawa, Hiroko AU - Fukasawa H AD - Department of Obstetrics and Gynecology, Yamanashi Red Cross Hospital, Kawaguchiko, JPN. LA - eng PT - Journal Article PT - Review DEP - 20260928 PL - United States TA - Cureus JT - Cureus JID - 101596737 PMC - PMC13714951 OTO - NOTNLM OT - gestational trophoblastic disease OT - intraoperative reporting OT - laparoscopy OT - morcellation OT - peritoneal washing OT - robotic surgery OT - scoping review OT - specimen retrieval OT - uterine manipulator COIS- Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/28 CRDT- 2026/10/29 05:47 PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:47 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - 10.7759/cureus.117071 [doi] PST - epublish SO - Cureus. 2026 Sep 28;18(9):e117071. doi: 10.7759/cureus.117071. eCollection 2026 Sep. PMID- 42953277 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1468-6996 (Print) IS - 1878-5514 (Electronic) IS - 1468-6996 (Linking) VI - 27 IP - 1 DP - 2026 TI - Emission control of longitudinal optical phonon resonant thermal terahertz radiation from two-dimensional surface metal micro-structures on GaAs. PG - 2724893 LID - 10.1080/14686996.2026.2724893 [doi] LID - 2724893 AB - Thermal emission of radiation resonant with the longitudinal optical (LO) phonon (LORE) has been studied using surface metal line-and-space structures on semiconductors. The emission perpendicular to the surface and the independence of the peak photon energy on emission direction are significant features, unlike those of surface phonon polaritons (SPhP). However, this emission is restricted to the optical polarization perpendicular to the stripes. In this research, two-dimensional (2-D) square-lattice and ring-type surface structures are fabricated and almost unpolarized LORE spectra are observed at 8.5 THz at 630 K, persisting to 323 K. Although the local spectrum minima due to optical absorption via SPhP show dependence on structural periods, the LORE peak energy is found to be robust. The LORE intensity averaged over polarizations is nearly twice that of the one-dimensional structure. In contrast, when fabricating a rectangular emission window with the side length short enough for the LO-phonon coherence, no LORE is observed. A finite-difference time-domain calculation suggests that this phenomenon is due to the disappearance of electric dipoles. Thus, two-dimensional structures enable control over the allowance and prohibition of THz wave emission, as well as its polarization. CI - (c) 2026 The Author(s). Published by National Institute for Materials Science in partnership with Taylor & Francis Group. FAU - Oshima, Takumi AU - Oshima T AD - Graduate School of Science and Engineering, Chiba University, Chiba, Japan. FAU - Yoshikawa, Daiki AU - Yoshikawa D AD - Graduate School of Science and Engineering, Chiba University, Chiba, Japan. FAU - Ishitani, Yoshihiro AU - Ishitani Y AUID- ORCID: 0000-0001-6445-2565 AD - Graduate School of Science and Engineering, Chiba University, Chiba, Japan. LA - eng PT - Journal Article DEP - 20260921 PL - United States TA - Sci Technol Adv Mater JT - Science and technology of advanced materials JID - 101614420 PMC - PMC13715342 OAB - Polarization-independent continuous-wave terahertz emission resonant with longitudinal optical phonons, using two-dimensional surface-metal microstructures on a GaAs wafer gives a promising mechanism for thermally stable THz emitters. OABL- eng OTO - NOTNLM OT - Longitudinal optical phonon OT - control of emission and exitinction by selecting the size of 2D emission window OT - polarization free by two dimendional structures OT - radiation emission by phonon OT - surface structures in the region keeping LO phonon coherence COIS- No potential conflict of interest was reported by the author(s). EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/21 CRDT- 2026/10/29 05:47 PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:47 [entrez] PHST- 2026/09/21 00:00 [pmc-release] AID - 2724893 [pii] AID - 10.1080/14686996.2026.2724893 [doi] PST - epublish SO - Sci Technol Adv Mater. 2026 Sep 21;27(1):2724893. doi: 10.1080/14686996.2026.2724893. eCollection 2026. PMID- 42953276 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1941-9066 (Print) IS - 1941-9074 (Electronic) IS - 1941-9074 (Linking) VI - 19 IP - 1 DP - 2026 TI - Iron-Based Contrast for the Heart: Clinical Promise of Ferumoxytol-Enhanced CMR. PG - 7 LID - 10.1007/s12410-026-09618-5 [doi] LID - 7 AB - PURPOSE OF REVIEW: Ferumoxytol is an ultrasmall superparamagnetic iron oxide (USPIO) nanoparticle that was originally approved by the U.S. FDA for treating iron deficiency anemia in patients with chronic kidney disease. It has since emerged as an off-label, versatile intravascular contrast agent for cardiovascular magnetic resonance (CMR) imaging, and in 2025, received FDA approval for brain imaging. This review provides an overview of ferumoxytol-enhanced CMR applications primarily focused on the heart, outlines its key imaging and pharmacokinetic properties, and summarizes its diagnostic safety profile. RECENT FINDINGS: Unlike gadolinium-based contrast agents (GBCAs), which rapidly wash out from the circulation, ferumoxytol has high r(1) and r(2) relaxivities and remains confined to the intravascular compartment for prolonged periods. Once metabolized by the reticuloendothelial system, its iron core is incorporated into the body's hematopoietic pathways. These unique properties enable steady-state and delayed-phase imaging, simplify complex imaging workflows, and facilitate accelerated multidimensional cardiac imaging methods. Emerging applications for ferumoxytol-enhanced CMR include myocardial perfusion assessment, multidimensional imaging, vascular and hemodynamic evaluation, and comprehensive structural heart disease imaging. SUMMARY: Ferumoxytol expands the clinical applications of CMR by enabling imaging techniques that are not feasible with conventional GBCAs and broadening imaging options for patients in whom GBCA administration may be less desirable based on individualized clinical risk-benefit assessment, including selected patients with renal insufficiency, very young children, and pregnant women. CI - (c) The Author(s) 2026. FAU - Pogosyan, Arutyun AU - Pogosyan A AD - Division of Cardiology, David Geffen School of Medicine at UCLA and VA Greater Los Angeles Healthcare System, Los Angeles, CA USA. ROR: https://ror.org/05xcarb80. GRID: grid.417119.b. ISNI: 0000 0001 0384 5381 AD - Department of Radiological Sciences, David Geffen School of Medicine at UCLA, Los Angeles, CA USA. ROR: https://ror.org/046rm7j60. GRID: grid.19006.3e. ISNI: 0000 0000 9632 6718 AD - Department of Bioengineering, University of California, Los Angeles, CA USA. ROR: https://ror.org/046rm7j60. GRID: grid.19006.3e. ISNI: 0000 0001 2167 8097 FAU - Liu, Yuehong AU - Liu Y AD - Department of Radiological Sciences, David Geffen School of Medicine at UCLA, Los Angeles, CA USA. ROR: https://ror.org/046rm7j60. GRID: grid.19006.3e. ISNI: 0000 0000 9632 6718 FAU - Patel, Jasmine A AU - Patel JA AD - Division of Cardiology, David Geffen School of Medicine at UCLA and VA Greater Los Angeles Healthcare System, Los Angeles, CA USA. ROR: https://ror.org/05xcarb80. GRID: grid.417119.b. ISNI: 0000 0001 0384 5381 FAU - Mahmoudi, Mostafa AU - Mahmoudi M AD - Division of Cardiology, David Geffen School of Medicine at UCLA and VA Greater Los Angeles Healthcare System, Los Angeles, CA USA. ROR: https://ror.org/05xcarb80. GRID: grid.417119.b. ISNI: 0000 0001 0384 5381 AD - Department of Radiological Sciences, David Geffen School of Medicine at UCLA, Los Angeles, CA USA. ROR: https://ror.org/046rm7j60. GRID: grid.19006.3e. ISNI: 0000 0000 9632 6718 AD - Department of Bioengineering, University of California, Los Angeles, CA USA. ROR: https://ror.org/046rm7j60. GRID: grid.19006.3e. ISNI: 0000 0001 2167 8097 FAU - Xu, Hang AU - Xu H AD - Division of Cardiology, David Geffen School of Medicine at UCLA and VA Greater Los Angeles Healthcare System, Los Angeles, CA USA. ROR: https://ror.org/05xcarb80. GRID: grid.417119.b. ISNI: 0000 0001 0384 5381 AD - Department of Radiological Sciences, David Geffen School of Medicine at UCLA, Los Angeles, CA USA. ROR: https://ror.org/046rm7j60. GRID: grid.19006.3e. ISNI: 0000 0000 9632 6718 AD - Department of Bioengineering, University of California, Los Angeles, CA USA. ROR: https://ror.org/046rm7j60. GRID: grid.19006.3e. ISNI: 0000 0001 2167 8097 FAU - Finn, J Paul AU - Finn JP AD - Department of Radiological Sciences, David Geffen School of Medicine at UCLA, Los Angeles, CA USA. ROR: https://ror.org/046rm7j60. GRID: grid.19006.3e. ISNI: 0000 0000 9632 6718 AD - Department of Radiological Sciences, University of California at Los Angeles, Peter V. Ueberroth Building Suite 3371, 10945 Le Conte Ave., Los Angeles, CA 90095-7206 USA. ROR: https://ror.org/046rm7j60. GRID: grid.19006.3e. ISNI: 0000 0001 2167 8097 FAU - Nguyen, Kim-Lien AU - Nguyen KL AD - Division of Cardiology, David Geffen School of Medicine at UCLA and VA Greater Los Angeles Healthcare System, Los Angeles, CA USA. ROR: https://ror.org/05xcarb80. GRID: grid.417119.b. ISNI: 0000 0001 0384 5381 AD - Department of Radiological Sciences, David Geffen School of Medicine at UCLA, Los Angeles, CA USA. ROR: https://ror.org/046rm7j60. GRID: grid.19006.3e. ISNI: 0000 0000 9632 6718 AD - Department of Bioengineering, University of California, Los Angeles, CA USA. ROR: https://ror.org/046rm7j60. GRID: grid.19006.3e. ISNI: 0000 0001 2167 8097 AD - David Geffen School of Medicine at UCLA, VA Greater Los Angeles Healthcare System, 11301 Wilshire Blvd, MC 111E, Los Angeles, CA 90073 USA. ROR: https://ror.org/05xcarb80. GRID: grid.417119.b. ISNI: 0000 0001 0384 5381 LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - United States TA - Curr Cardiovasc Imaging Rep JT - Current cardiovascular imaging reports JID - 101484775 PMC - PMC13714125 OTO - NOTNLM OT - Cardiovascular magnetic resonance OT - Contrast agents OT - Ferumoxytol OT - Magnetic resonance imaging OT - USPIO COIS- Competing InterestsThe authors declare no competing interests. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/27 CRDT- 2026/10/29 05:47 PHST- 2026/07/07 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:47 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 9618 [pii] AID - 10.1007/s12410-026-09618-5 [doi] PST - ppublish SO - Curr Cardiovasc Imaging Rep. 2026;19(1):7. doi: 10.1007/s12410-026-09618-5. Epub 2026 Oct 27. PMID- 42953271 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2352-5878 (Electronic) IS - 2352-5878 (Linking) VI - 52 DP - 2026 Nov TI - Use of thigh-based recipient vessels for free flap reconstruction in gustilo anderson type IIIC lower extremity open fractures: Description of two cases. PG - 652-656 LID - 10.1016/j.jpra.2026.09.033 [doi] AB - BACKGROUND: Free tissue transfer is central to reconstruction of Gustilo IIIC lower-extremity injuries, but recipient vessel selection is challenging when local vasculature is compromised. We describe two Gustilo IIIC injuries reconstructed using thigh-based recipient vessels. CASE PRESENTATIONS: Case 1: A 50-year-old man sustained an open tibial fracture with popliteal artery thrombosis and extensive tibial vessel injury. Following revascularization and serial debridement, a 25 x 15 cm tibial defect was reconstructed with a contralateral anterolateral thigh (ALT) free flap to the descending genicular artery (DGA), using an interposition great saphenous vein graft. The flap survived without complication.Case 2: A 35-year-old man sustained an open tibial fracture with complete popliteal artery transection requiring vascular reconstruction. A 20 x 14 cm defect was initially reconstructed with a free latissimus dorsi flap to the DGA using an interposition vein graft, complicated by venous thrombosis and flap loss. Subsequent reconstruction with an ALT flap and radial forearm flow-through flap to the superficial femoral vessels (SFA) achieved successful soft-tissue coverage. DISCUSSION: The DGA provides an accessible recipient outside the primary zone of injury, with consistent anatomy and suitable calibre for microsurgical anastomosis. Its location facilitates a two-team approach, avoids popliteal fossa exploration, and may reduce interference with subsequent skeletal procedures. The SFA is a valuable alternative recipient vessel, offering robust and reliable inflow with a good arterial pressure head. CONCLUSION: Thigh-based recipient vessels, particularly the DGA and SFA, should be considered in severe lower-limb trauma when conventional below-knee recipient vessels are unavailable or compromised. CI - (c) 2026 The Authors. FAU - Zeng, Faith Ai Ping AU - Zeng FAP AD - Plastics, Reconstructive and Aesthetics Surgery Service, Department of General Surgery, Khoo Teck Puat Hospital, Singapore. FAU - Chan, Sally Shin Jie AU - Chan SSJ AD - Plastics, Reconstructive and Aesthetics Surgery Service, Department of General Surgery, Khoo Teck Puat Hospital, Singapore. FAU - Wu, Yijun AU - Wu Y AD - Plastics, Reconstructive and Aesthetics Surgery Service, Department of General Surgery, Khoo Teck Puat Hospital, Singapore. FAU - Hwee, Jolie Jingyi AU - Hwee JJ AD - Plastics, Reconstructive and Aesthetics Surgery Service, Department of General Surgery, Khoo Teck Puat Hospital, Singapore. LA - eng PT - Case Reports PT - Journal Article DEP - 20260930 PL - Netherlands TA - JPRAS Open JT - JPRAS open JID - 101680420 PMC - PMC13715301 OTO - NOTNLM OT - Descending genicular artery OT - Free flap OT - Gustilo iiic lower extremity injury OT - Lower limb reconstruction OT - Lower limb trauma OT - Orthoplastics OT - Superficial femoral artery OT - Thigh-based recipient vessels EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/30 CRDT- 2026/10/29 05:46 PHST- 2026/08/27 00:00 [received] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:46 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - S2352-5878(26)00349-9 [pii] AID - 10.1016/j.jpra.2026.09.033 [doi] PST - epublish SO - JPRAS Open. 2026 Sep 30;52:652-656. doi: 10.1016/j.jpra.2026.09.033. eCollection 2026 Nov. PMID- 42953266 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 0970-9134 (Print) IS - 0973-7723 (Electronic) IS - 0970-9134 (Linking) VI - 42 IP - 11 DP - 2026 Nov TI - CHAMPION-AF and left atrial appendage closure: non-inferiority or a trade-off in risk? PG - 1225-1228 LID - 10.1007/s12055-026-02271-z [doi] AB - The Watchman FLX versus Novel Oral Anticoagulation for Embolic Protection in the Management of Patients with Non-Valvular Atrial Fibrillation (CHAMPION-AF) trial compared left atrial appendage (LAA) closure using the Watchman FLX device with non-vitamin K antagonist oral anticoagulants (NOACs) in patients with non-valvular atrial fibrillation eligible for long-term anticoagulation. LAA closure demonstrated non-inferiority for the composite endpoint of cardiovascular death, stroke, or systemic embolism and reduced non-procedure-related bleeding. However, interpretation is limited by a permissive non-inferiority margin, reliance on a composite endpoint, exclusion of procedural bleeding from the primary safety outcome, and a numerical excess of ischaemic strokes. Overall, CHAMPION-AF represents a shift in risk profile rather than therapeutic equivalence, supporting LAA closure in selected patients rather than as a routine first-line alternative to NOAC therapy. CI - (c) Indian Association of Cardiovascular-Thoracic Surgeons 2026. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. FAU - Shales, Sufina AU - Shales S AUID- ORCID: 0000-0002-2480-6338 AD - Narayana Health, Bengaluru, India. ROR: https://ror.org/05kx1ke03. GRID: grid.416504.2. ISNI: 0000 0004 1796 819X LA - eng PT - Journal Article DEP - 20260905 PL - India TA - Indian J Thorac Cardiovasc Surg JT - Indian journal of thoracic and cardiovascular surgery JID - 8700105 PMC - PMC13713930 OTO - NOTNLM OT - CHAMPION-AF OT - Left atrial appendage closure OT - NOACs OT - Non-valvular atrial fibrillation COIS- Competing interestsSufina Shales serves as an Assistant Editor for the Indian Journal of Thoracic and Cardiovascular Surgery and was not involved in the peer review or editorial decision-making process for this manuscript. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2027/11/01 CRDT- 2026/10/29 05:46 PHST- 2026/08/03 00:00 [received] PHST- 2026/08/06 00:00 [revised] PHST- 2026/08/21 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:46 [entrez] PHST- 2027/11/01 00:00 [pmc-release] AID - 2271 [pii] AID - 10.1007/s12055-026-02271-z [doi] PST - ppublish SO - Indian J Thorac Cardiovasc Surg. 2026 Nov;42(11):1225-1228. doi: 10.1007/s12055-026-02271-z. Epub 2026 Sep 5. PMID- 42953260 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - The association between telemedicine and patient outcomes in osteoarthritis and inflammatory arthritis: a systematic review and meta-analysis of randomized controlled trials. PG - 1897145 LID - 10.3389/fmed.2026.1897145 [doi] LID - 1897145 AB - BACKGROUND: Osteoarthritis (OA) and inflammatory arthritis (IA) are prevalent chronic musculoskeletal disorders that cause persistent pain, functional impairment, and reduced quality of life. Telemedicine offers a potential strategy for long-term arthritis management, but the strength of the supporting evidence remains uncertain. This systematic review and meta-analysis evaluated the efficacy of telemedicine interventions in patients with OA and IA. METHODS: We searched PubMed, Embase, Cochrane Library, Web of Science, and WHO ICTRP up to 10 July 2026 for randomized controlled trials (RCTs). Two reviewers independently conducted study selection, data extraction, and risk-of-bias assessment using the RoB 2.0 tool. Random-effects meta-analysis was performed to calculate standardized mean differences (SMDs) with 95% confidence intervals (CIs) and 95% prediction intervals (PIs). The certainty of evidence was rated using the GRADE framework. RESULTS: Twenty-four RCTs enrolling 2,262 patients (1,568 with OA and 694 with IA) were included. Telemedicine was associated with improvements in pain (SMD = -0.66, 95% CI -1.00 to -0.32; 95% PI -1.90 to 0.58), quality of life (SMD = 0.42, 95% CI 0.12 to 0.71; 95% PI -0.60 to 1.44), WOMAC scores (SMD = -0.78, 95% CI -1.19 to -0.37; 95% PI -2.25 to 0.69), and self-efficacy (SMD = 0.47, 95% CI 0.06 to 0.89; 95% PI -1.04 to 1.98). All primary outcomes showed substantial heterogeneity (I (2) > 75%), with 95% PIs that spanned the null value, indicating that the true effect in future studies could be zero or even negative. The self-efficacy finding lacked robustness in leave-one-out sensitivity analyses and was susceptible to the influence of individual studies. Evidence for IA was restricted to a small number of trials on rheumatoid arthritis and ankylosing spondylitis, and the overall certainty of evidence was rated very low to low. CONCLUSIONS: Current evidence suggests an association between telemedicine and improved outcomes in arthritis, but these findings are exploratory and limited by low evidence certainty, marked heterogeneity, and wide prediction intervals. Evidence for IA is insufficient to draw definitive conclusions. SYSTEMATIC REVIEW REGISTRATION: https://www.crd.york.ac.uk/PROSPERO/view/CRD420261401824, PROSPERO CRD420261401824. CI - (c) 2026 Zhang, Zhou, Wu, Guo, Lin and Zhao. FAU - Zhang, Yize AU - Zhang Y AD - Second Clinical Medical College, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China. FAU - Zhou, Meijie AU - Zhou M AD - School of Public Health, Hainan Medical University, Haikou, Hainan, China. FAU - Wu, Fangzhen AU - Wu F AD - Department of Rheumatology and Endocrinology, The Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China. FAU - Guo, Yanfen AU - Guo Y AD - Department of Rheumatology and Endocrinology, The Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China. FAU - Lin, Xiaorong AU - Lin X AD - Department of Rheumatology and Endocrinology, The Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China. FAU - Zhao, Zhongwen AU - Zhao Z AD - Department of Rheumatology and Endocrinology, The Second Affiliated Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, China. LA - eng PT - Journal Article PT - Systematic Review DEP - 20261014 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13713862 OTO - NOTNLM OT - inflammatory arthritis OT - meta-analysis OT - osteoarthritis OT - systematic review OT - telemedicine COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:46 PHST- 2026/06/01 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:46 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmed.2026.1897145 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 14;13:1897145. doi: 10.3389/fmed.2026.1897145. eCollection 2026. PMID- 42953258 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1687-8728 (Print) IS - 1687-8736 (Electronic) IS - 1687-8728 (Linking) VI - 2026 DP - 2026 TI - Ocular Injuries and Eye Safety Practices in Dental Settings: A Scoping Review. PG - 4079205 LID - 10.1155/ijod/4079205 [doi] LID - 4079205 AB - INTRODUCTION: Aerosols, chemicals, curing lights, dental therapeutic lasers and microbiological pollutants in dental clinics can cause eye infections and impair clinicians, patients and auxiliary vision. This scoping review explored the existing evidence on dental-related ocular injuries. OBJECTIVE: This scoping review aimed to map the evidence on various ocular injuries arising from dental procedures. METHODS: Only published data were searched in PubMed, Scopus, CINAHL (Ebsco) and Embase (Elsevier). English-language literary data was collected from 2005 to the present. Studies conducted on all patients and dentists, and dental personnel directly involved in dental treatments, working at dental clinics and dental hospitals, were included. The front-desk employee and non-direct dental personnel/non-operating auxiliaries were exempted. Systemic disease-related and allergic ocular injuries were excluded. Data were imported into Rayyan for critical analysis. Two independent reviewers thoroughly scanned and analysed the selected references for inclusion criteria. A third reviewer was included to resolve article eligibility disputes between the two reviewers. A PRISMA flow diagram and narrative summary were presented to represent the collected data. Data on ocular injuries at the dental set-up were summarised and explained. RESULTS: Database search retrieved 49 complete articles. After complete screening, only 8 full articles were included that met the inclusion criteria. The results were obtained from original studies and case reports. Six main themes had emerged from thematic content analysis. CONCLUSIONS: The conclusions of this scoping review will increase awareness among dental clinicians and personnel regarding ocular injuries and support the implementation of appropriate precautionary measures. CI - Copyright (c) 2026 Roma Mascarenhas et al. International Journal of Dentistry published by John Wiley & Sons Ltd. FAU - Mascarenhas, Roma AU - Mascarenhas R AUID- ORCID: 0000-0003-4159-1233 AD - Department of Conservative Dentistry and Endodontics Manipal College of Dental Sciences Mangalore Manipal Academy of Higher Education Manipal India manipal.edu. FAU - Rao, Shushma AU - Rao S AUID- ORCID: 0000-0002-0855-0846 AD - Department of Public Health Dentistry Srinivas Institute of Dental Sciences Mangalore Affiliated to Rajiv Gandhi University of Health Sciences Bangalore Mangalore Karnataka India srinivasgroup.com. FAU - Mala, Kundabala AU - Mala K AUID- ORCID: 0000-0003-2407-1877 AD - Department of Conservative Dentistry and Endodontics Manipal College of Dental Sciences Mangalore Manipal Academy of Higher Education Manipal India manipal.edu. FAU - Rashmi, Shashi U AU - Rashmi SU AUID- ORCID: 0000-0001-8906-6353 AD - Department of Conservative Dentistry and Endodontics Manipal College of Dental Sciences Manipal Academy of Higher Education Manipal India manipal.edu. FAU - Mahajan, Prajval AU - Mahajan P AUID- ORCID: 0009-0003-0753-1832 AD - Manipal College of Dental Sciences Mangalore Manipal Academy of Higher Education Manipal India manipal.edu. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - United States TA - Int J Dent JT - International journal of dentistry JID - 101524183 PMC - PMC13714086 OTO - NOTNLM OT - dentistry OT - health OT - ocular injuries OT - ophthalmology OT - well-being COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/27 CRDT- 2026/10/29 05:46 PHST- 2026/01/13 00:00 [received] PHST- 2026/06/10 00:00 [revised] PHST- 2026/08/07 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:46 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - IJOD4079205 [pii] AID - 10.1155/ijod/4079205 [doi] PST - epublish SO - Int J Dent. 2026 Oct 27;2026:4079205. doi: 10.1155/ijod/4079205. eCollection 2026. PMID- 42953257 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2832-3637 (Electronic) IS - 2832-3637 (Linking) VI - 4 IP - 10 DP - 2026 Oct 26 TI - Kinetic Modeling and Radiomics Analysis of the Heterogeneity of Tumor-Associated Macrophages and Metabolism in Head and Neck Cancer. PG - 2302-2312 LID - 10.1021/cbmi.6c00065 [doi] AB - Purpose: Tumor-associated macrophages (TAMs) play an important role in the initiation and progression of head and neck squamous cell carcinoma (HNSCC). Herein, we evaluated the tumor tissue heterogeneity uptake of a CD163+ PET tracer, [(64)Cu]-ICT-01, and compared it with [(18)F]-FDG using kinetic modeling and radiomics analysis in a mouse oral carcinoma 1 (MOC1) HNSCC mouse model. Methods: MOC1 cells were implanted on the flanks of four C57BL/6 mice. At 3 weeks post tumor implantation, these mice underwent a 60 min dynamic scan post-injection of [(18)F]-FDG and then another 60 min dynamic scan the next day post-injection of [(64)Cu]-ICT-01. Nine tumor volumes were delineated using a region-growing algorithm, which were further divided into tissue volumes based on tracer uptake using k-means clustering. Kinetic modeling was performed using compartmental models and graphical Logan/Patlak. Standardized uptake value (SUV) PET images were generated using time windows of 1-10 min and 45- 60 min. A total of 91 textural radiomic features were obtained using Pyradiomics, and key features were selected using the coefficient of variance (CV). Results: k-means clustering identified three distinct tissue uptakes for [(64)Cu]-ICT-01, and only two tissues with high and low uptakes for [(18)F]-FDG. The 2TC4K and Logan models were found to be suitable models for modeling the kinetics of [(64)Cu]-ICT-01. [(18)F]-FDG generally yielded greater variances in quantitative parameters across tissues within the tumors than those of [(64)Cu]-ICT-01, except for K (1). However, greater variances in radiomic features were obtained for [(64)Cu]-ICT-01 than for [(18)F]-FDG. [(64)Cu]-ICT-01 also yielded six more features with CV > 0.7 compared to [(18)F]-FDG. Conclusion: [(64)Cu]-ICT-01 showed greater differences in uptake kinetics and textural information, while [(18)F]-FDG provided greater differences in quantitative values across the tissues within the tumor. [(64)Cu]-ICT-01 and [(18)F]-FDG showed the heterogeneous nature of the tumor microenvironment, and their combination would enable us to understand the tumor biology and hence improve tumor diagnosis. CI - (c) 2026 The Authors. Co-published by Nanjing University and American Chemical Society. FAU - Nai, Ying-Hwey AU - Nai YH AUID- ORCID: 0000-0002-6634-8785 AD - Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, United States. RINGGOLD: 12275 FAU - Heo, Gyu Seong AU - Heo GS AUID- ORCID: 0000-0001-6200-4742 AD - Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, United States. RINGGOLD: 12275 FAU - Madrid, Priscella AU - Madrid P AD - Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, United States. RINGGOLD: 12275 FAU - Sultan, Deborah AU - Sultan D AD - Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, United States. RINGGOLD: 12275 FAU - Laforest, Richard AU - Laforest R AD - Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, United States. RINGGOLD: 12275 FAU - Gropler, Robert AU - Gropler R AD - Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, United States. RINGGOLD: 12275 FAU - Liu, Yongjian AU - Liu Y AUID- ORCID: 0000-0002-1118-1535 AD - Mallinckrodt Institute of Radiology, Washington University School of Medicine, St Louis, Missouri 63110, United States. RINGGOLD: 12275 LA - eng PT - Journal Article DEP - 20260530 PL - United States TA - Chem Biomed Imaging JT - Chemical & biomedical imaging JID - 9918574388106676 PMC - PMC13715349 OTO - NOTNLM OT - CD163 OT - kinetic modeling OT - metabolism OT - positron emission tomography (PET) OT - radiomics OT - tissue heterogeneity evaluation EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/05/30 CRDT- 2026/10/29 05:46 PHST- 2026/03/09 00:00 [received] PHST- 2026/05/20 00:00 [revised] PHST- 2026/05/22 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:46 [entrez] PHST- 2026/05/30 00:00 [pmc-release] AID - 10.1021/cbmi.6c00065 [doi] PST - epublish SO - Chem Biomed Imaging. 2026 May 30;4(10):2302-2312. doi: 10.1021/cbmi.6c00065. eCollection 2026 Oct 26. PMID- 42953253 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 17 DP - 2026 TI - Decoding molecular heterogeneity in lupus nephritis: integrating single-cell and spatial multi-omics for endotype-driven precision therapeutics. PG - 1987126 LID - 10.3389/fimmu.2026.1987126 [doi] LID - 1987126 AB - Lupus nephritis (LN) develops in 40-60% of patients with systemic lupus erythematosus (SLE) and remains the leading cause of renal morbidity and progression to end-stage renal disease (ESRD). While the upstream immunological events initiating LN-including loss of self-tolerance, pathogenic autoantibody production, glomerular immune complex deposition, and type I interferon (IFN) activation-are broadly shared, accumulating evidence demonstrates that these convergent triggers give rise to molecularly heterogeneous tissue injury programs at the renal level. This fundamental divergence provides a mechanistic explanation for the inconsistent therapeutic responses observed with standardized immunosuppressive protocols. Advances in single-cell transcriptomics, spatial gene expression mapping, CITE-seq, and cross-platform multi-omic integration have deconvolved this heterogeneity into at least five recognizable tissue-level injury programs: (i) an IFN-gamma-polarized macrophage-tubular epithelial cell co-activation circuit, (ii) a Tfh-B cell ectopic germinal center pathway, (iii) a neutrophil extracellular trap (NET)-driven inflammatory cascade, (iv) a podocyte-autonomous injury program, and (v) a TREM2+ macrophage-orchestrated fibrotic program. Concurrently, multiplexed urinary biomarker platforms are under development as non-invasive surrogates for tissue-level pathogenic activity. This review synthesizes current understanding of disease mechanisms, molecular profiling evidence, biomarker candidates, and mechanism-directed therapies corresponding to each tissue injury program. We map existing and investigational agents-including anifrolumab, obinutuzumab, voclosporin, CAR-T cell therapy, and complement inhibitors-to their mechanistic targets and delineate the design requirements for prospective biomarker-stratified trials essential to validating molecularly guided treatment selection. CI - Copyright (c) 2026 Zhou, Xiao and Jian. FAU - Zhou, Jun AU - Zhou J AD - Department of Traditional Chinese Medicine, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi, China. AD - Department of Traditional Chinese Medicine, Gaoxin Branch of the First Affiliated Hospital, Nanchang University, Nanchang, Jiangxi, China. FAU - Xiao, Wei AU - Xiao W AD - Department of Traditional Chinese Medicine, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi, China. FAU - Jian, Hui AU - Jian H AD - Department of Traditional Chinese Medicine, Jiangxi University of Chinese Medicine, Nanchang, Jiangxi, China. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 0 (Biomarkers) SB - IM MH - Humans MH - *Lupus Nephritis/genetics/immunology/therapy/drug therapy MH - Single-Cell Analysis MH - Multiomics MH - Animals MH - Biomarkers MH - *Precision Medicine/methods PMC - PMC13714975 OTO - NOTNLM OT - lupus nephritis OT - molecular disease heterogeneity OT - precision renal medicine OT - single-cell transcriptomics OT - spatial multi-omics OT - urinary biomarkers COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:45 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:45 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fimmu.2026.1987126 [doi] PST - epublish SO - Front Immunol. 2026 Oct 14;17:1987126. doi: 10.3389/fimmu.2026.1987126. eCollection 2026. PMID- 42953251 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2691-3704 (Electronic) IS - 2691-3704 (Linking) VI - 6 IP - 10 DP - 2026 Oct 26 TI - Predictive Design of Fulvalene-Bridged Bimetallic Molecular Solar-Thermal Fuels. PG - 5648-5662 LID - 10.1021/jacsau.6c00919 [doi] AB - Molecular solar-thermal (MOST) energy storage requires the simultaneous optimization of photocharging efficiency, energy storage capacity, and thermal persistence. The diruthenium fulvalene complex (Fv)-Ru(2)(CO)(4) remains the only organometallic MOST fuel that supports a fully closed photothermal cycle, enabled by triplet-surface mediated photocharging and singlet-surface mediated thermal discharge. Isoelectronic metal substitution typically disrupts this closed cycle, either by quenching the essential triplet photocharging pathway or by kinetically trapping the high-energy isomeryet these failures have remained without a unifying mechanistic rationale, and whether viable compositions exist beyond (Fv)-Ru(2)(CO)(4) has been unknown. Here, we introduce a dual-gate framework that elevates metal-pair selection from an empirical choice to a predictive and rational design parameter. The framework defines two complementary filters: a ground-state Storage-Stability Gate, in which stored free energy and the thermal back-reaction barrier are intrinsically coupled, and an excited-state Photo Gate, requiring Franck-Condon-proximal access to the isomerizing triplet. Screening a 21-member library of Group 6/Group 8 homo- and heterobimetallic dimers with these two filters identifies (Fv)-OsW-(CO)(5) as a previously unrecognized, synthetically accessible MOST candidate. This proof-of-concept demonstrates that viable MOST candidates can be found beyond the (Fv)-Ru(2)(CO)(4) benchmark through rational design rather than empirical substitution. The framework simultaneously explains why isoelectronic substitution has historically failed, delineating viable and nonviable regions of bimetallic design space and providing transferable, mechanism-guided principles for MOST design. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Ganguly, Gaurab AU - Ganguly G AUID- ORCID: 0000-0002-3357-0435 AD - Institute of Theoretical Chemistry, Faculty of Chemistry, University of Vienna, Wahringer Str. 17, 1090 Vienna, Austria. RINGGOLD: 27258 FAU - Gonzalez, Leticia AU - Gonzalez L AUID- ORCID: 0000-0001-5112-794X AD - Institute of Theoretical Chemistry, Faculty of Chemistry, University of Vienna, Wahringer Str. 17, 1090 Vienna, Austria. RINGGOLD: 27258 LA - eng PT - Journal Article DEP - 20260816 PL - United States TA - JACS Au JT - JACS Au JID - 101775714 PMC - PMC13714945 OTO - NOTNLM OT - bimetallic complexes OT - computational screening OT - intersystem crossing OT - molecular photoswitches OT - molecular solar-thermal (MOST) energy storage OT - periodic trends EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/08/16 CRDT- 2026/10/29 05:45 PHST- 2026/06/11 00:00 [received] PHST- 2026/08/06 00:00 [revised] PHST- 2026/08/10 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:45 [entrez] PHST- 2026/08/16 00:00 [pmc-release] AID - 10.1021/jacsau.6c00919 [doi] PST - epublish SO - JACS Au. 2026 Aug 16;6(10):5648-5662. doi: 10.1021/jacsau.6c00919. eCollection 2026 Oct 26. PMID- 42953249 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Development and validation of a machine learning-based prediction model for emergence agitation in geriatric patients undergoing orthopedic surgery: a prospective cohort study. PG - 1903005 LID - 10.3389/fmed.2026.1903005 [doi] LID - 1903005 AB - OBJECTIVE: This study aims to identify factors associated with emergence agitation in geriatric patients undergoing orthopedic surgery and to develop a machine learning model for predicting the risk of emergence agitation. METHODS: This prospective cohort study included 612 geriatric patients who underwent elective orthopedic surgery at the First Affiliated Hospital of Jinzhou Medical University in Liaoning Province between April 2025 and April 2026. The patients were randomly divided into a training set (n = 430) and a test set (n = 182) at a 7:3 ratio. Least absolute shrinkage and selection operator (LASSO) regression and multivariable logistic regression analysis were used to identify predictors. Six machine learning algorithms were employed to develop prediction models. Model performance was evaluated using the area under the receiver operating characteristic curve (AUC), calibration curve, and decision curve analysis (DCA). The optimal model was interpreted using Shapley Additive Explanations (SHAP). RESULTS: The incidence of emergence agitation among geriatric patients who underwent orthopedic surgery was 27.61%. Preoperative anxiety, preoperative cognitive function, dexmedetomidine use, intraoperative hypothermia, intraoperative blood loss, and postoperative NRS pain score were identified as key predictors. The random forest (RF) model demonstrated the best performance, achieving an AUC of 0.905, accuracy of 0.819, sensitivity of 0.820, specificity of 0.818, F1 score of 0.713, and Brier score of 0.111 on the test set. DCA showed that the model had the highest clinical net benefit. SHAP analysis revealed that intraoperative blood loss was the most significant predictor, followed by postoperative NRS pain score and preoperative cognitive function. CONCLUSION: We developed and internally validated six machine learning models to predict the risk of emergence agitation in geriatric patients undergoing orthopedic surgery. The RF model achieved the best performance. Following successful external validation in multicenter cohorts, the model may assist clinicians in identifying patients at increased risk of emergence agitation during early PACU recovery and in prioritizing intensified monitoring and timely supportive management. CI - (c) 2026 Wang, Zhao, Huang, Tang, Liu, Wei and Zhang. FAU - Wang, Ping AU - Wang P AD - School of Nursing, Jinzhou Medical University, Jinzhou, Liaoning, China. FAU - Zhao, Jinxuan AU - Zhao J AD - School of Nursing, Jinzhou Medical University, Jinzhou, Liaoning, China. FAU - Huang, Jinhui AU - Huang J AD - School of Nursing, Jinzhou Medical University, Jinzhou, Liaoning, China. FAU - Tang, Yingying AU - Tang Y AD - The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China. FAU - Liu, Jiayi AU - Liu J AD - The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China. FAU - Wei, Xin AU - Wei X AD - The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China. FAU - Zhang, Mengshu AU - Zhang M AD - The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13713869 OTO - NOTNLM OT - emergence agitation OT - geriatric patients OT - machine learning OT - orthopedic surgery OT - prediction model OT - risk factors COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:44 PHST- 2026/06/08 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:44 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmed.2026.1903005 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 14;13:1903005. doi: 10.3389/fmed.2026.1903005. eCollection 2026. PMID- 42953232 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-462X (Print) IS - 1664-462X (Electronic) IS - 1664-462X (Linking) VI - 17 DP - 2026 TI - AgroXAI: a multi-XAI deep learning system for explainable multi-species plant disease classification. PG - 1965848 LID - 10.3389/fpls.2026.1965848 [doi] LID - 1965848 AB - Automated plant disease classification can benefit from models that provide both predictive performance and interpretable evidence for their decisions. This study presents AgroXAI, a Multi-XAI deep learning framework for explainable multi-species plant disease classification under a controlled, leakage-aware evaluation protocol. A publicly available dataset containing 2,815 leaf images from seven plant species was organized into 14 joint species-health classes and partitioned into fixed group-disjoint training, validation, and held-out test sets containing 1,970, 422, and 423 images, respectively. Preprocessing and architecture selection were conducted using only the training and validation partitions, leading to the AgroX-Swin classifier based on Swin Transformer-Tiny with 384 x 384 Original RGB inputs. Explainability was quantitatively evaluated using Grad-CAM++, SHAP, LIME, and their complementary fusions on a predefined class-balanced subset of 42 held-out images. Across five independently initialized training runs, AgroX-Swin achieved a mean held-out accuracy of 87.19% and macro-F1 of 0.8300 +/- 0.0122, while the five-model probability ensemble achieved 87.94% accuracy and 0.8355 macro-F1. Deterministic aggregation of the same 14-class predictions yielded 99.29% species-recognition accuracy and 88.42% binary health-status accuracy, with a diseased-class F1-score of 0.9160. Temperature scaling reduced the representative model's expected calibration error from 0.0792 to 0.0310. At the 20% perturbation level, Grad-CAM++ + LIME produced the largest mean confidence drop (0.2540), while Grad-CAM++ showed the highest transformation stability (0.8371) and SHAP + LIME showed the highest attribution-burden stability (0.7954), demonstrating complementary rather than uniformly superior behavior among individual and fused explanation methods. Model-randomization analysis further examined the dependence of Grad-CAM++ explanations on learned model parameters. AgroXAI provides an integrated framework combining leakage-aware model development, multi-seed training-stability assessment, held-out classification, calibration, statistical uncertainty analysis, and quantitative evaluation of complementary XAI methods. The resulting attribution maps are interpreted as prediction-relevant visual explanations rather than validated lesion localization or quantitative disease-severity estimates. External robustness and real-world agricultural generalization remain to be established using independently acquired field datasets. CI - Copyright (c) 2026 Kumar K. and Balaji. FAU - Kumar K, Sathish AU - Kumar K S AD - School of Computer Science and Engineering, Vellore Institute of Technology, Vellore, India. FAU - Balaji, G N AU - Balaji GN AD - School of Computer Science and Engineering, Vellore Institute of Technology, Vellore, India. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Plant Sci JT - Frontiers in plant science JID - 101568200 PMC - PMC13713886 OTO - NOTNLM OT - Swin transformer OT - deep learning OT - explainability validation OT - explainable artificial intelligence (XAI) OT - multi-XAI fusion OT - plant leaf disease detection COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:43 PHST- 2026/08/12 00:00 [received] PHST- 2026/09/11 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:43 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpls.2026.1965848 [doi] PST - epublish SO - Front Plant Sci. 2026 Oct 14;17:1965848. doi: 10.3389/fpls.2026.1965848. eCollection 2026. PMID- 42953225 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2297-1769 (Print) IS - 2297-1769 (Electronic) IS - 2297-1769 (Linking) VI - 13 DP - 2026 TI - Testing minimally invasive imaging methods for phenotypic sex determination in immature frogs. PG - 1934243 LID - 10.3389/fvets.2026.1934243 [doi] LID - 1934243 AB - Amphibians are regularly encountered in exotic veterinary practice, and knowledge of their sex can be extremely important for various programs for breeding and species conservation, as well as for veterinary care. However, sex identification remains challenging because many amphibians lack secondary sexual characteristics until reaching maturity, and genetic sex may not always correspond to phenotypic sex. Consequently, visual inspection of the gonads is often the most reliable method for identifying sex in amphibians. However, non-invasive imaging methods for gonad examination have not been formally validated in juvenile amphibians, where the small size of the gonads may limit visualization success. In our study, we attempted to determine the phenotypic sex of small-sized, immature and mature agile frogs (Rana dalmatina) using three non-invasive imaging methods: general veterinary ultrasound, native computed tomography (CT), and magnetic resonance imaging (MRI). Ovaries in immature females were not unambiguously recognizable with any of the tested methods, but became clearly visible during oocyte development, enabling identification in mature females with MRI and ultrasonography. Testes were identifiable in both immature and mature males by MRI but not by ultrasonography or CT. Weighing the costs and benefits of each method in light of our results, we make recommendations for further research into non-invasive sexing of juvenile amphibians. CI - Copyright (c) 2026 Ujhegyi, Bokony, Ziszisz, Kovacs, Horvath, Rozsnyik, Limboczki, Lini, Berkei, Nemeshazi, Kasler, Miko, Mathe, Szigeti and Balogh. FAU - Ujhegyi, Nikolett AU - Ujhegyi N AD - Department of Evolutionary Ecology, Plant Protection Institute, HUN-REN Centre for Agricultural Research, Budapest, Hungary. AD - Department of Wildlife Biology and Management, Hungarian University of Agriculture and Life Sciences, Godollo, Hungary. FAU - Bokony, Veronika AU - Bokony V AD - Department of Evolutionary Ecology, Plant Protection Institute, HUN-REN Centre for Agricultural Research, Budapest, Hungary. FAU - Ziszisz, Arisz AU - Ziszisz A AD - Department of Exotic Animal-, Wildlife-, Fish- and Honeybee Medicine, University of Veterinary Medicine Budapest, Budapest, Hungary. FAU - Kovacs, Noemi AU - Kovacs N AD - Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary. AD - In vivo Imaging Advanced Core Facility, Hungarian Centre of Excellence for Molecular Medicine, Szeged, Hungary. FAU - Horvath, Ildiko AU - Horvath I AD - Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary. AD - In vivo Imaging Advanced Core Facility, Hungarian Centre of Excellence for Molecular Medicine, Szeged, Hungary. FAU - Rozsnyik, Maya Zsofia AU - Rozsnyik MZ AD - Csipet Csapat Veterinary Center, Budapest, Hungary. FAU - Limboczki, Richard AU - Limboczki R AD - Csipet Csapat Veterinary Center, Budapest, Hungary. FAU - Lini, Akos Benedek AU - Lini AB AD - Csipet Csapat Veterinary Center, Budapest, Hungary. FAU - Berkei, Gabor AU - Berkei G AD - Helvetic Clinics, Budapest, Hungary. FAU - Nemeshazi, Edina AU - Nemeshazi E AD - Department of Evolutionary Ecology, Plant Protection Institute, HUN-REN Centre for Agricultural Research, Budapest, Hungary. AD - Konrad Lorenz Institute of Ethology, Department of Interdisciplinary Life Sciences, University of Veterinary Medicine Vienna, Vienna, Austria. FAU - Kasler, Andrea AU - Kasler A AD - Department of Evolutionary Ecology, Plant Protection Institute, HUN-REN Centre for Agricultural Research, Budapest, Hungary. AD - Department of Systematic Zoology and Ecology, ELTE Eotvos Lorand University, Budapest, Hungary. FAU - Miko, Zsanett AU - Miko Z AD - Department of Evolutionary Ecology, Plant Protection Institute, HUN-REN Centre for Agricultural Research, Budapest, Hungary. AD - Department of Systematic Zoology and Ecology, ELTE Eotvos Lorand University, Budapest, Hungary. FAU - Mathe, Domokos AU - Mathe D AD - Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary. AD - In vivo Imaging Advanced Core Facility, Hungarian Centre of Excellence for Molecular Medicine, Szeged, Hungary. FAU - Szigeti, Krisztian AU - Szigeti K AD - Department of Biophysics and Radiation Biology, Semmelweis University, Budapest, Hungary. AD - In vivo Imaging Advanced Core Facility, Hungarian Centre of Excellence for Molecular Medicine, Szeged, Hungary. FAU - Balogh, Emese AU - Balogh E AD - Department of Zoology, University of Veterinary Medicine Budapest, Budapest, Hungary. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Vet Sci JT - Frontiers in veterinary science JID - 101666658 PMC - PMC13714919 OTO - NOTNLM OT - Rana dalmatina OT - amphibians OT - computed tomography OT - gonad examination OT - juveniles OT - magnetic resonance (MR) OT - sexual maturity age OT - ultrasonography COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:43 PHST- 2026/07/10 00:00 [received] PHST- 2026/09/09 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:43 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fvets.2026.1934243 [doi] PST - epublish SO - Front Vet Sci. 2026 Oct 14;13:1934243. doi: 10.3389/fvets.2026.1934243. eCollection 2026. PMID- 42953221 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2516-1571 (Electronic) IS - 2096-5303 (Print) IS - 2516-1571 (Linking) VI - 9 IP - 4 DP - 2026 Dec TI - Artesunate ameliorates acute pancreatitis by enhancing p62-mediated zymophagy through non-canonical NRF2 activation. PG - pbag029 LID - 10.1093/pcmedi/pbag029 [doi] LID - pbag029 AB - OBJECTIVE: Zymophagy is a selective autophagy that eliminates activated trypsinogen during acute pancreatitis (AP). However, the upstream regulatory mechanisms and therapeutic potential of zymophagy remain poorly understood. This study investigated the mechanisms of zymophagy activation and its protective role in AP, using artesunate (AS) as a pharmacological tool. METHODS: AP models were induced by cerulein combined with lipopolysaccharide and evaluated by histopathological examination and enzymatic assays. Gene expression was knocked down with specific shRNA or siRNA, and expression levels were detected by real-time polymerase chain reaction (PCR). Zymophagy and its regulatory mechanisms were characterized through multiple experiments, and molecular interactions were validated by co-localization, immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), thermal shift assay, and molecular docking. RESULTS: AS mitigated AP in both in vivo and in vitro models by inhibiting trypsinogen hyperactivation in acinar cells. This protective effect was mediated by enhanced Atg5-mediated zymophagy activation via a p62-dependent and vacuole membrane protein 1 (VMP1)-ubiquitin-involved pathway, which promotes the targeted engulfment and degradation of trypsinogen. Mechanistically, AS binds the PB1 domain of p62, strengthens its interaction with Kelch-like ECH-associated protein 1 (Keap1), triggers ubiquitin-p62-mediated autophagic degradation of Keap1, facilitates the nuclear translocation of nuclear factor erythroid 2-related factor 2 (NRF2) and its selective binding to the antioxidant response element in the p62 promoter, and ultimately upregulates p62 transcription. CONCLUSION: Zymophagy is activated via a VMP1-ubiquitin-p62-dependent and Atg5-mediated canonical autophagy pathway. AS ameliorates AP by enhancing p62-mediated zymophagy via non-canonical NRF2 activation, which provides mechanistic evidence for therapeutic targeting of acinar zymophagy in AP management. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the West China School of Medicine & West China Hospital of Sichuan University. FAU - Cen, Yanyan AU - Cen Y AD - Department of Pharmacology, College of Pharmacy, Army Medical University, Chongqing 400038, China. FAU - Xiong, Yalan AU - Xiong Y AD - Department of Pharmacology, College of Pharmacy, Army Medical University, Chongqing 400038, China. FAU - Liu, Chenxu AU - Liu C AD - Department of Pharmacology, College of Pharmacy, Army Medical University, Chongqing 400038, China. FAU - Wang, Pan AU - Wang P AD - Department of Pharmacology, College of Pharmacy, Army Medical University, Chongqing 400038, China. FAU - Zhu, Ting AU - Zhu T AD - Department of Pharmacology, College of Pharmacy, Army Medical University, Chongqing 400038, China. FAU - Li, Fei AU - Li F AD - Biomedical Analysis Center, Army Medical University, Chongqing 400038, China. FAU - Zhang, Haigang AU - Zhang H AD - Department of Pharmacology, College of Pharmacy, Army Medical University, Chongqing 400038, China. FAU - Cao, Wenxuan AU - Cao W AD - Department of Pharmaceutical Analysis, College of Pharmacy, Army Medical University, Chongqing 400038, China. FAU - Deng, Youcai AU - Deng Y AUID- ORCID: 0000-0001-5488-8576 AD - Department of Pharmacology, College of Pharmacy, Army Medical University, Chongqing 400038, China. FAU - Pan, Xichun AU - Pan X AD - Department of Pharmacology, College of Pharmacy, Army Medical University, Chongqing 400038, China. LA - eng PT - Journal Article DEP - 20261013 PL - England TA - Precis Clin Med JT - Precision clinical medicine JID - 101739592 PMC - PMC13714909 OTO - NOTNLM OT - Keap1 OT - NRF2 OT - acute pancreatitis OT - artesunate OT - p62 OT - zymophagy COIS- The authors declare no competing interests. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/13 CRDT- 2026/10/29 05:43 PHST- 2026/07/30 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:43 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - pbag029 [pii] AID - 10.1093/pcmedi/pbag029 [doi] PST - epublish SO - Precis Clin Med. 2026 Oct 13;9(4):pbag029. doi: 10.1093/pcmedi/pbag029. eCollection 2026 Dec. PMID- 42953214 OWN - NLM STAT- Publisher LR - 20261029 IS - 1556-3316 (Print) IS - 1556-3324 (Electronic) IS - 1556-3316 (Linking) DP - 2026 Oct 26 TI - Knee Arthroscopy for Wear-Induced Synovitis of a Recalled Implant Is Associated With a High Rate of Failure and Fails to Avoid Need for Revision TKA. PG - 15563316261492330 LID - 10.1177/15563316261492330 [doi] LID - 15563316261492330 AB - BACKGROUND: Polyethylene wear-induced synovitis and osteolysis are well-recognized complications following total knee arthroplasty (TKA) and have been of particular concern in the setting of recalled Exactech implants. While knee arthroscopy (KA) has been proposed as a minimally invasive option to address intra-articular debris and synovitis, its effectiveness in preventing disease progression and implant failure remains unclear. PURPOSES: The purpose of this study was to evaluate the clinical and radiographic outcomes of KA in patients with recalled Exactech TKA presenting with polyethylene wear-induced synovitis. Specifically, we assessed rates of reoperation, revision arthroplasty, and progression of osteolysis following arthroscopic intervention. METHODS: A retrospective chart review was conducted on 38 patients who underwent KA after primary TKA with recalled Exactech implants between January 2022 and January 2023. Patient demographics, clinical outcomes, and radiographic and magnetic resonance imaging (MRI) findings were analyzed by a high-volume, fellowship-trained arthroplasty surgeon. RESULTS: Within 1 year of follow-up, 4/38 patients (10.5%) underwent revision TKA, 4/38 (10.5%) patients underwent repeat KA, and 6/38 patients (15.8%) demonstrated progressive osteolysis on follow-up radiographs, resulting in an overall failure rate of 14/38 patients (36.8%). Of the remaining 30 patients who had not undergone revision TKA or repeat KA, 29 of 30 patients (96.7%) had one of the following findings: possible component loosening (5/30), progressive radiolucent lines (25/30), and worsening osteolysis on follow-up radiographs (15/30). Additionally, upon review of patient post-arthroscopy MRIs, 28/30 patients (93.3%) had documentation of persistent/progressive polymeric-induced synovitis or persistent/progressive osteolysis. CONCLUSION: The high failure rate reported in the current study suggests that KA with debridement is not an appropriate treatment for patients with polyethylene wear-induced synovitis and osteolysis following the implantation of a recalled Exactech TKA. Delay of the surgery can result in more severe bone defects. In patients with wear-induced synovitis but without osteolysis or implant loosening, a polyethylene insert exchange is the preferred alternative treatment. LEVEL OF EVIDENCE: Prognostic study level IV. CI - (c) The Author(s) 2026. FAU - Schiller, Nicholas C AU - Schiller NC AUID- ORCID: 0009-0007-6802-9052 AD - Adult Reconstruction and Joint Replacement Service, Hospital for Special Surgery, New York, NY, USA. FAU - Hughes, Andrew AU - Hughes A AD - Adult Reconstruction and Joint Replacement Service, Hospital for Special Surgery, New York, NY, USA. FAU - Selkridge, Isaiah AU - Selkridge I AD - Adult Reconstruction and Joint Replacement Service, Hospital for Special Surgery, New York, NY, USA. FAU - Sculco, Thomas P AU - Sculco TP AD - Adult Reconstruction and Joint Replacement Service, Hospital for Special Surgery, New York, NY, USA. FAU - Sculco, Peter K AU - Sculco PK AD - Adult Reconstruction and Joint Replacement Service, Hospital for Special Surgery, New York, NY, USA. FAU - Boettner, Friedrich AU - Boettner F AUID- ORCID: 0000-0002-7906-9486 AD - Adult Reconstruction and Joint Replacement Service, Hospital for Special Surgery, New York, NY, USA. LA - eng PT - Journal Article DEP - 20261026 PL - United States TA - HSS J JT - HSS journal : the musculoskeletal journal of Hospital for Special Surgery JID - 101273938 PMC - PMC13713920 OTO - NOTNLM OT - knee arthroscopy OT - osteolysis OT - polyethylene wear OT - revision total knee arthroplasty OT - total knee arthroplasty COIS- The authors declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: Thomas P. Sculco, MD, is a member of the editorial board of HSS Journal. He did not participate in the peer review or decision-making process for this submission. Additional potential conflicts of interest include royalties from Exactech, the manufacturer of the implant discussed in this article, and relationships with American Journal of Orthopedics, J. Robert Gladden Society, and Orthopaedic Research and Education Foundation. Peter K. Sculco, MD, reports relationships with Envosi, Osgenic, BetterPT, HS2, LLC, HSS ASC Development Network, LLC Intellijoint Surgical, Inc, Joint Effort Administrative Services Organization, LLC, Ortho AI, Parvizi Surgical Innovation, LLC, and Zimmer Biomet. Friedrich Boettner, MD, declares relationships with OrthoDevelopment, Smith & Nephew, Medacta, Symbios, OrthoForum GmbH, AccuJoint, Inc, HS2, LLC Joint Effort Administrative Services Organization, LLC, Syntellix AG. The other authors declare no potential conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 PMCR- 2027/10/26 CRDT- 2026/10/29 05:43 PHST- 2026/04/07 00:00 [received] PHST- 2026/06/18 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:43 [entrez] PHST- 2027/10/26 00:00 [pmc-release] AID - 10.1177_15563316261492330 [pii] AID - 10.1177/15563316261492330 [doi] PST - aheadofprint SO - HSS J. 2026 Oct 26:15563316261492330. doi: 10.1177/15563316261492330. PMID- 42953213 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2976-5390 (Electronic) IS - 2976-5390 (Linking) VI - 4 IP - 10 DP - 2026 Oct TI - Medicare Part D utilization management for negotiated drugs and within-class comparators, 2019-2026. PG - qxag290 LID - 10.1093/haschl/qxag290 [doi] LID - qxag290 AB - INTRODUCTION: The Inflation Reduction Act requires Medicare drug plans to cover medications selected for federal price negotiation once negotiated prices take effect, and by 2026 all 10 first-round drugs were covered by every plan. Plans retain the ability to require prior authorization, step therapy, and/or quantity limits as formulary management tools. METHODS: Using national Medicare Part D formulary files, 2019-2026, we compared restrictions on 9 of the 10 negotiated medications with those on similar drugs in the same classes not selected for negotiation. Estimates were weighted by plan enrollment. RESULTS: Weighted by enrollment, step therapy on negotiated diabetes medications fell to zero by 2025 and rose to 5.0% in 2026, while comparators stayed below 1%; negotiated anticoagulants rose to 4.8%. Cost sharing is set by formulary tier rather than by drug and did not change. Prior authorization was near-absent in the cardiometabolic classes and near-universal in the specialty classes, with no change associated with negotiation. CONCLUSION: Guaranteed coverage does not guarantee access. In the first year of negotiated prices, universal listing coexisted with a return of step therapy on negotiated cardiometabolic drugs that comparators did not mirror, while what beneficiaries paid at the pharmacy was unchanged. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of Health Affairs Publishing, LLC. FAU - Jain, Vidur AU - Jain V AUID- ORCID: 0009-0000-9454-0515 AD - Division of Endocrinology, Diabetes, & Nutrition, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, United States. FAU - McCoy, Rozalina G AU - McCoy RG AUID- ORCID: 0000-0002-2289-3183 AD - Division of Endocrinology, Diabetes, & Nutrition, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, United States. AD - University of Maryland Institute for Health Computing, North Bethesda, MD 20852, United States. LA - eng PT - Journal Article DEP - 20261019 PL - England TA - Health Aff Sch JT - Health affairs scholar JID - 9918627882906676 PMC - PMC13714910 OTO - NOTNLM OT - Medicare Part D OT - drug price negotiation OT - formulary coverage OT - inflation reduction act OT - prior authorization OT - utilization management COIS- Conflicts of interest: Please see ICMJE form(s) for author conflicts of interest. These have been provided as supplementary materials. In the last 36 months, Dr. R.G.M. has received unrelated research support from the NIH (including NIDDK, NIA, NHLBI, and NCI), the Patient-Centered Outcomes Research Institute (PCORI), the Department of Defense, and the American Diabetes Association. She served as a consultant to NCQA and the Yale-New Haven Health System's Center for Outcomes Research and Evaluation and has received speaking honoraria and travel support from the American Diabetes Association. Mr. V.J. declares that he has no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/19 CRDT- 2026/10/29 05:43 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:43 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - qxag290 [pii] AID - 10.1093/haschl/qxag290 [doi] PST - epublish SO - Health Aff Sch. 2026 Oct 19;4(10):qxag290. doi: 10.1093/haschl/qxag290. eCollection 2026 Oct. PMID- 42953203 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2691-3704 (Electronic) IS - 2691-3704 (Linking) VI - 6 IP - 10 DP - 2026 Oct 26 TI - Terazosin Activates or Inhibits Human Phosphoglycerate Kinase 1 on a Dose-Dependent Basis: Mechanistic and Structural Insights by NMR. PG - 5623-5630 LID - 10.1021/jacsau.6c00823 [doi] AB - Phosphoglycerate kinase 1 (PGK1) is a key metabolic enzyme responsible for ATP production, and its dysregulation is linked to the Warburg effect in cancer and to neurodegenerative diseases such as Parkinson's. Recent studies indicate that terazosin, an FDA-approved drug, enhances PGK1-mediated ATP production. However, X-ray crystallography indicates that terazosin binds at a site that partially overlaps the nucleotide-binding site, thereby inhibiting PGK1. In this study, we employed solution-state nuclear magnetic resonance (NMR) chemical shift titrations to confirm that terazosin binds at a site that partially overlaps the nucleotide-binding pocket of PGK1, stabilizing the enzyme in its open conformation, which is accessible to substrates and products. This binding shifts the equilibrium toward the open state, enhancing product release at low concentrations while blocking substrate binding at higher concentrations. These findings are strengthened by the 3D NMR(2) structure determination of the PGK1-terazosin complex, using selective unlabeling combined with subsequent partial amino acid assignments, which validates the available X-ray structure. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Butikofer, Matthias AU - Butikofer M AUID- ORCID: 0000-0002-7900-8288 AD - Institute for Molecular Physical Science, Vladimir Prelog Weg 2, 8093 Zurich, Switzerland. AD - Department of Pharmaceutical Sciences, University of Vienna, Josef-Holaubek-Platz 2, A-1090 Vienna, Austria. FAU - Torres, Felix AU - Torres F AD - Institute for Molecular Physical Science, Vladimir Prelog Weg 2, 8093 Zurich, Switzerland. AD - NexMR AG, Wiesenstrasse 10A, 8952 Schlieren, Switzerland. FAU - Ghosh, Dhiman AU - Ghosh D AD - Institute for Molecular Physical Science, Vladimir Prelog Weg 2, 8093 Zurich, Switzerland. FAU - Lorz, Nils AU - Lorz N AD - Institute for Molecular Physical Science, Vladimir Prelog Weg 2, 8093 Zurich, Switzerland. FAU - Riek, Roland AU - Riek R AD - Institute for Molecular Physical Science, Vladimir Prelog Weg 2, 8093 Zurich, Switzerland. FAU - Orts, Julien AU - Orts J AUID- ORCID: 0000-0003-3287-1532 AD - Department of Pharmaceutical Sciences, University of Vienna, Josef-Holaubek-Platz 2, A-1090 Vienna, Austria. LA - eng PT - Journal Article DEP - 20260924 PL - United States TA - JACS Au JT - JACS Au JID - 101775714 PMC - PMC13714833 OTO - NOTNLM OT - Chemical shift perturbation OT - NMR spectroscopy OT - Parkinson's disease OT - Phosphoglycerate kinase 1 (PGK1) OT - Protein-ligand binding OT - Terazosin OT - Warburg effect EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/24 CRDT- 2026/10/29 05:42 PHST- 2026/05/26 00:00 [received] PHST- 2026/09/11 00:00 [revised] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:42 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - 10.1021/jacsau.6c00823 [doi] PST - epublish SO - JACS Au. 2026 Sep 24;6(10):5623-5630. doi: 10.1021/jacsau.6c00823. eCollection 2026 Oct 26. PMID- 42953191 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-2295 (Electronic) IS - 1664-2295 (Linking) VI - 17 DP - 2026 TI - Advances in artificial intelligence-driven MRI research in Alzheimer's disease. PG - 1943265 LID - 10.3389/fneur.2026.1943265 [doi] LID - 1943265 AB - Alzheimer's disease (AD), the most prevalent neurodegenerative disorder, poses substantial challenges for early diagnosis and longitudinal monitoring-two cornerstones of effective clinical management and therapeutic intervention. Magnetic resonance imaging (MRI), owing to its non-invasiveness and high spatial resolution, has emerged as an indispensable tool in AD research. In recent years, artificial intelligence (AI), particularly deep learning (DL) and machine learning (ML) techniques, has demonstrated remarkable potential for automating MRI analysis, extracting subtle imaging features, and supporting objective disease prediction. This review comprehensively summarizes the latest advances in AI-driven MRI applications for AD diagnosis, staging, prognosis, and adjuvant therapeutic monitoring. It highlights key progress in AI model development, multimodal data processing, and clinical translational value. Furthermore, current bottlenecks, including data heterogeneity, model interpretability, and real-world deployment, are systematically discussed, and future directions are outlined to enhance the precision and clinical utility of AI-assisted MRI in AD. Overall, AI-MRI has evolved from laboratory validation toward early clinical adoption, with explainability and multi-center generalizability identified as critical priorities in the next research phase. CI - Copyright (c) 2026 Deng, Zeng, Ou, Zhou, Gou and Huang. FAU - Deng, Lihua AU - Deng L AD - Department of Radiology, The First People's Hospital of Neijiang, Neijaing, Sichuan, China. AD - Department of Radiology, No. 926 Hospital, Joint Logistics Support Force of PLA, Kaiyuan, Yunnan, China. AD - 7T Magnetic Resonance Imaging Translational Medical Center, Department of Radiology, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing, China. FAU - Zeng, Yan AU - Zeng Y AD - Department of Ultrasound, The First People's Hospital of Neijiang, Neijiang, Sichuan, China. FAU - Ou, Peiling AU - Ou P AD - 7T Magnetic Resonance Imaging Translational Medical Center, Department of Radiology, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing, China. AD - Department of Radiology, Graduate School of Peking Union Medical College, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. FAU - Zhou, Suyi AU - Zhou S AD - 7T Magnetic Resonance Imaging Translational Medical Center, Department of Radiology, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing, China. FAU - Gou, Yu AU - Gou Y AD - Department of Radiology, No. 926 Hospital, Joint Logistics Support Force of PLA, Kaiyuan, Yunnan, China. FAU - Huang, Cong AU - Huang C AD - Department of Radiology, The First People's Hospital of Neijiang, Neijaing, Sichuan, China. AD - Department of Radiology, No. 926 Hospital, Joint Logistics Support Force of PLA, Kaiyuan, Yunnan, China. AD - Department of Nuclear Medicine, The First People's Hospital of Neijiang, Neijiang, Sichuan, China. LA - eng PT - Journal Article PT - Review DEP - 20261008 PL - Switzerland TA - Front Neurol JT - Frontiers in neurology JID - 101546899 SB - IM MH - Humans MH - *Alzheimer Disease/diagnostic imaging MH - *Magnetic Resonance Imaging/methods MH - *Artificial Intelligence MH - *Neuroimaging/methods MH - Machine Learning MH - Deep Learning PMC - PMC13714817 OTO - NOTNLM OT - Alzheimer's disease OT - artificial intelligence OT - deep learning OT - early diagnosis OT - image analysis OT - machine learning OT - magnetic resonance imaging COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/08 CRDT- 2026/10/29 05:41 PHST- 2026/07/20 00:00 [received] PHST- 2026/08/24 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:41 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - 10.3389/fneur.2026.1943265 [doi] PST - epublish SO - Front Neurol. 2026 Oct 8;17:1943265. doi: 10.3389/fneur.2026.1943265. eCollection 2026. PMID- 42953190 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 17 DP - 2026 TI - Coal dust-stimulated macrophage-derived extracellular vesicles promote thyroid cancer cell phenotypes involving miR-205-5p, CAMK1G, and NF-kappaB-associated signaling. PG - 1856770 LID - 10.3389/fimmu.2026.1856770 [doi] LID - 1856770 AB - BACKGROUND: Occupational coal dust exposure causes persistent pulmonary inflammation, but the mechanisms by which it may influence thyroid cancer progression remain unclear. Macrophage-derived extracellular vesicles (EVs) may participate in intercellular communication under particulate stress. OBJECTIVE: This study investigated whether EVs released by coal dust-stimulated macrophages alter thyroid cancer cell behavior and examined the involvement of miR-205-5p, CAMK1G, and NF-kappaB-associated signaling. METHODS: Coal dust was physicochemically characterized before use. EVs were isolated from PMA-differentiated THP-1 macrophages following coal dust exposure and characterized by transmission electron microscopy, nanoparticle tracking analysis, and Western blotting. Small-RNA sequencing, bulk RNA sequencing, TCGA-THCA analysis, and the GSE274674 single-cell dataset were integrated with RT-qPCR, nuclear-cytoplasmic fractionation, dual-luciferase reporter assays, and genetic rescue experiments. Coal dust-exposed C57BL/6J mice and PBMC-humanized NSFG mice bearing TPC-1 xenografts were used for in vivo evaluation. RESULTS: PKH26-labeled EV-associated material was detected in TPC-1 cells, and EV preparations increased cell viability, clonogenic growth, migration, and invasion while reducing apoptosis. miR-205-5p was enriched in these EVs and accumulated predominantly in the nuclear fraction of recipient cells. Inhibition of miR-205-5p attenuated the EV-associated malignant phenotypes. Integrated transcriptomic analyses prioritized CAMK1G and NF-kappaB-related signaling. EV treatment increased endogenous CAMK1G expression and the phosphorylation of p65 and IkappaBalpha. CAMK1G knockdown reduced these changes, whereas CAMK1G re-expression partially restored the effects suppressed by miR-205-5p inhibition. The reporter assay showed sequence-dependent repression of the isolated CAMK1G 3'-UTR construct, whereas endogenous-expression and rescue experiments supported a positive functional association; therefore, these findings are not interpreted as a simple direct positive regulatory relationship. In mice, coal dust exposure was associated with pulmonary abnormalities, altered serum FT4 and TPO-Ab levels, increased thyroid CAMK1G expression, and enhanced pulmonary CD206 immunoreactivity. In PBMC-humanized mice, coal dust exposure or experimentally administered macrophage-derived EVs increased xenograft growth, whereas CAMK1G knockdown attenuated tumor burden. CONCLUSION: These findings support a functional association between coal dust-stimulated macrophage-derived EV signaling and thyroid cancer progression involving miR-205-5p, CAMK1G, and NF-kappaB-associated activity. However, direct physiological trafficking of endogenous lung-derived EVs to thyroid or tumor tissue was not demonstrated. CI - Copyright (c) 2026 Zhao, Ma, Zhang and Zhang. FAU - Zhao, Feng AU - Zhao F AD - The First Affiliated Hospital of Anhui University of Science and Technology, Huainan, China. FAU - Ma, Tianqing AU - Ma T AD - The First Affiliated Hospital of Anhui University of Science and Technology, Huainan, China. FAU - Zhang, Chi AU - Zhang C AD - School of Medicine, Anhui University of Science & Technology, Huainan, China. FAU - Zhang, Hongzhen AU - Zhang H AD - Anhui University of Science and Technology, Huainan, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 0 (MicroRNAs) RN - 0 (NF-kappa B) RN - 0 (Coal) RN - 0 (MIR205, human) RN - 0 (Dust) SB - IM MH - *MicroRNAs/genetics/metabolism MH - Humans MH - Animals MH - *NF-kappa B/metabolism MH - Signal Transduction MH - Mice MH - *Macrophages/metabolism/immunology MH - *Thyroid Neoplasms/pathology/metabolism/genetics/etiology MH - *Extracellular Vesicles/metabolism MH - *Coal/adverse effects MH - Cell Line, Tumor MH - *Dust MH - Phenotype MH - Mice, Inbred C57BL PMC - PMC13713815 OTO - NOTNLM OT - CAMK1G OT - NF-kappaB signaling OT - coal dust OT - macrophage-derived extracellular vesicles OT - miR-205-5p OT - occupational Exposure OT - thyroid cancer COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:41 PHST- 2026/04/15 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:41 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fimmu.2026.1856770 [doi] PST - epublish SO - Front Immunol. 2026 Oct 14;17:1856770. doi: 10.3389/fimmu.2026.1856770. eCollection 2026. PMID- 42953187 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2297-1769 (Print) IS - 2297-1769 (Electronic) IS - 2297-1769 (Linking) VI - 13 DP - 2026 TI - Metagenomic characterization of microbial communities associated with alphaherpesvirinae detection in magnificent frigatebirds from French Guiana. PG - 1866497 LID - 10.3389/fvets.2026.1866497 [doi] LID - 1866497 AB - INTRODUCTION: A population of Magnificent Frigatebirds (Fregata magnificens, Suliformes) inhabiting French Guiana has been under investigation since 2005 due to high chick mortality rates associated with nodular proliferative skin lesions, characterized by featherless areas on the body. An initial study conducted in 2009 reported the presence of an alphaherpesvirus detected in a crust from a single individual in the same frigatebird population. However, the cause of the outbreaks and the associated high mortality in the frigatebird population remained undetermined. METHODS: Almost 15 years later, in 2024, we collected beak swabs, cloacal swabs, and blood samples from 14 animals. Six animals were clinically healthy, while eight showed clinical signs including skin crusts, hyperkeratosis on the eyes, corneal thickening, and signs of distress. To investigate the potential causes of those signs, we screened the samples for herpesviruses. To characterize bacterial and viral communities and investigate potential co-infections, we selected four beak swabs for metagenomic analysis: two from herpesvirus-positive individuals and two from herpesvirus-negative controls (one healthy and one with clinical signs), and sequenced them using metagenomic Next Generation Sequencing (NGS). RESULTS AND DISCUSSION: Two individuals tested positive for herpesviruses in beak swabs, one healthy and one with clinical signs. Analysis of the bacterial community revealed that the most abundant phyla were Actinobacteria, Proteobacteria, Firmicutes, Bacteroidetes, and Fusobacteria, which typically inhabit the gut microbiota. All reported bacterial phyla were detected in every sequenced sample. At the viral level, among non-bacteriophage viruses, Orthoherpesviridae showed the highest abundance in PCR-positive animals, followed by Adenoviridae- and Hepadnaviridae-related sequences. Sequence analysis of the DNA-directed DNA polymerase (DdDp) region of Orthoherpesviridae identified two sequences: one with 98.1% nucleotide identity to sequences reported in the same geographic area in 2005, and the other with 72.1% identity to a vulture herpesvirus from India. The bacterial and viral populations detected were largely similar between animals with and without clinical signs. Due to the limited sample size, it was not possible to determine whether the detected microorganisms were associated with the observed clinical signs. CI - Copyright (c) 2026 Di Bartolo, Sebastiano, Vaccari, Suffredini, Boni, Costantini and De Sabato. FAU - Di Bartolo, Ilaria AU - Di Bartolo I AD - Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanita, Rome, Italy. FAU - Sebastiano, Manrico AU - Sebastiano M AD - Behavioural Ecology and Ecophysiology Group, Department of Biology, University of Antwerp, Universiteitsplein, Wilrijk, Belgium. FAU - Vaccari, Gabriele AU - Vaccari G AD - Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanita, Rome, Italy. FAU - Suffredini, Elisabetta AU - Suffredini E AD - Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanita, Rome, Italy. FAU - Boni, Arianna AU - Boni A AD - Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanita, Rome, Italy. FAU - Costantini, David AU - Costantini D AD - Department of Ecological and Biological Sciences, University of Tuscia, Viterbo, Italy. FAU - De Sabato, Luca AU - De Sabato L AD - Department of Food Safety, Nutrition and Veterinary Public Health, Istituto Superiore di Sanita, Rome, Italy. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Vet Sci JT - Frontiers in veterinary science JID - 101666658 PMC - PMC13714930 OTO - NOTNLM OT - Fregata magnificens OT - frigatebird OT - herpesvirus OT - metagenomics OT - viral communities COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:41 PHST- 2026/04/27 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:41 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fvets.2026.1866497 [doi] PST - epublish SO - Front Vet Sci. 2026 Oct 14;13:1866497. doi: 10.3389/fvets.2026.1866497. eCollection 2026. PMID- 42953185 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-2392 (Print) IS - 1664-2392 (Electronic) IS - 1664-2392 (Linking) VI - 17 DP - 2026 TI - Forecasting accuracy is not enough: safety-oriented evaluation of neural digital twins prior to in-silico insulin and carbohydrate intervention in type 1 diabetes. PG - 1958918 LID - 10.3389/fendo.2026.1958918 [doi] LID - 1958918 AB - Neural networks trained on a single patient's continuous glucose monitoring (CGM) record are increasingly proposed as "digital twins" that could let clinicians rehearse insulin and carbohydrate decisions in silico before applying them to the patient. Before any such use, a candidate twin should respond to treatment in a direction consistent with established pharmacology: a model that merely extrapolates the recent glucose trend can report an excellent forecasting error while remaining effectively blind to insulin, and this failure is invisible to the root-mean-square error (RMSE) that dominates the glucose-forecasting literature. We examined this gap in two independent cohorts: a longitudinal, multimodal University of Manchester cohort (17 participants enrolled; 13 formed the Core analytical cohort after data-quality screening) and, as an independent replication of the protocol, the OhioT1DM dataset (12 participants). Patient-specific two-layer long short-term memory (LSTM) models were trained under a strict chronological 60/20/20 split and evaluated across five random seeds. A physiology-guided variant added insulin-on-board and carbohydrate-on-board states with a double-monotonicity training penalty encouraging insulin to lower and carbohydrate to raise predicted glucose. Two findings replicated across both cohorts and all seeds. First, on the accuracy axis, a physiology-free Vanilla LSTM had the lowest one-step RMSE (Manchester 7.00, Ohio 5.02 mg/dL; Friedman chi (2)(5) = 43.4 and 46.7, both p < 10 (-) (7)), and under two-hour rollout a non-learning persistence baseline beat every neural model at 60 and 120 minutes-even after rollout-aware training-so ranking twins by accuracy alone would select a model that cannot represent treatment at all. Second, on a graded insulin dose-response audit, the physiology-guided model produced the steepest correctly-directed (glucose-lowering) response among the primary configurations in both cohorts (slope significantly better than the unconstrained model: Holm-corrected p = 0.002 and 0.003), whereas removing the directional constraint left a wrong-signed response; a supplementary ablation without masking was steeper still, pointing to the directional penalty, not masking, as the driver. Its advantage over the simpler Vanilla model was significant on for this cohort dependence. Accuracy and treatment-response plausibility are separable properties; we provide a reproducible protocol auditing both before a neural twin is used for intervention simulation. CI - Copyright (c) 2026 Ibrahim, Banjar, Harper, Alarood and Alsolami. FAU - Ibrahim, Ahmed AU - Ibrahim A AD - Department of Computer Science and Artificial Intelligence, College of Computer Science and Engineering, University of Jeddah, Jeddah, Saudi Arabia. FAU - Banjar, Ameen AU - Banjar A AD - Department of Computer Science and Artificial Intelligence, College of Computer Science and Engineering, University of Jeddah, Jeddah, Saudi Arabia. AD - Department of Information Systems and Technology, College of Computer Science and Engineering, University of Jeddah, Jeddah, Saudi Arabia. FAU - Harper, Simon AU - Harper S AD - Department of Computer Science, The University of Manchester, Manchester, United Kingdom. FAU - Alarood, Ala AU - Alarood A AD - Department of Information Technology, College of Computer Science and Engineering, University of Jeddah, Jeddah, Saudi Arabia. FAU - Alsolami, Eesa AU - Alsolami E AD - Department of Cybersecurity, College of Computer Science and Engineering, University of Jeddah, Jeddah, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Endocrinol (Lausanne) JT - Frontiers in endocrinology JID - 101555782 RN - 0 (Insulin) RN - 0 (Blood Glucose) RN - 0 (Hypoglycemic Agents) RN - 0 (Dietary Carbohydrates) SB - IM MH - Humans MH - *Diabetes Mellitus, Type 1/drug therapy/blood MH - *Insulin/administration & dosage/therapeutic use MH - Continuous Glucose Monitoring MH - Female MH - Male MH - Adult MH - Computer Simulation MH - Blood Glucose/analysis MH - *Neural Networks, Computer MH - *Hypoglycemic Agents/therapeutic use/administration & dosage MH - Forecasting MH - *Dietary Carbohydrates/administration & dosage MH - Blood Glucose Self-Monitoring/methods MH - Longitudinal Studies MH - Prediction Algorithms MH - Long Short Term Memory PMC - PMC13714816 OTO - NOTNLM OT - LSTM OT - continuous glucose monitoring OT - digital twin OT - dose-response audit OT - glucose forecasting OT - in-silico intervention simulation OT - treatment-response plausibility OT - type 1 diabetes COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:41 PHST- 2026/08/05 00:00 [received] PHST- 2026/09/12 00:00 [revised] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:41 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fendo.2026.1958918 [doi] PST - epublish SO - Front Endocrinol (Lausanne). 2026 Oct 14;17:1958918. doi: 10.3389/fendo.2026.1958918. eCollection 2026. PMID- 42953182 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 17 DP - 2026 TI - Medullary epithelium-free areas in the rat thymus are specialized niches enriched for mature thymocytes and distinct stromal subsets. PG - 1945985 LID - 10.3389/fimmu.2026.1945985 [doi] LID - 1945985 AB - The thymic medulla provides the microenvironment for negative selection, late thymocyte maturation, and thymocyte egress, and is generally characterized by widespread distribution of medullary thymic epithelial cells (mTECs). In contrast, the rat thymic medulla contains medullary epithelium-free areas (mEFAs), but the cellular composition and functional significance of these regions remain unclear. Here, to address these questions, we combined spatial transcriptomic data with thymocyte and stromal scRNA-seq reference datasets using robust cell-type decomposition (RCTD) to characterize mEFAs. These analyses revealed that more mature phenotypes within the CD4SP, CD8SP, and regulatory T-cell lineages were preferentially localized in mEFAs, whereas immature SP subsets were enriched in medullary epithelium-containing areas. The newly identified rat thymic mesenchymal cell subsets TMC3 and TMC4 were also enriched in mEFAs. These subsets were broadly similar to mouse medullary fibroblasts but displayed distinct predicted interactions with SP thymocytes, including costimulatory molecule-receptor, chemokine-receptor, and ECM-integrin axes. In addition, venous endothelial cells (vECs) expressing portal endothelial cell markers were enriched in mEFAs. Spns2, which encodes a transporter that releases S1P from cells into the extracellular space, was preferentially expressed in both TMC4 and vEC subsets, suggesting that these cells may contribute to the formation of a local S1P gradient within mEFAs. These findings suggest that mEFAs may be specialized medullary niches associated with stromal organization, the localization of mature thymocytes, and thymic egress. CI - Copyright (c) 2026 Sawanobori and Ogawa. FAU - Sawanobori, Yasushi AU - Sawanobori Y AD - Department of Anatomy, Dokkyo Medical University, Mibu, Tochigi, Japan. FAU - Ogawa, Tadayuki AU - Ogawa T AD - Laboratory for Molecular Pathobiology, Research Center for Advanced Medical Science, Dokkyo Medical University, Mibu, Tochigi, Japan. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 SB - IM MH - Animals MH - *Thymus Gland/cytology/immunology/metabolism MH - *Thymocytes/cytology/immunology/metabolism MH - Rats MH - *Stromal Cells/metabolism/immunology/cytology MH - Epithelial Cells MH - Cell Differentiation MH - Spatial Transcriptomics PMC - PMC13713823 OTO - NOTNLM OT - bioinformatics OT - fibroblast OT - scRNA-seq OT - spatial transcriptomics OT - thymic mesenchymal cells OT - thymocyte OT - thymus COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:41 PHST- 2026/07/23 00:00 [received] PHST- 2026/08/22 00:00 [revised] PHST- 2026/09/02 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:41 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fimmu.2026.1945985 [doi] PST - epublish SO - Front Immunol. 2026 Oct 14;17:1945985. doi: 10.3389/fimmu.2026.1945985. eCollection 2026. PMID- 42953180 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 17 DP - 2026 TI - Case Report: Upadacitinib for Acrodermatitis continua of Hallopeau: a case series with literature review. PG - 1903377 LID - 10.3389/fimmu.2026.1903377 [doi] LID - 1903377 AB - BACKGROUND: Acrodermatitis continua of Hallopeau (ACH) is a relatively rare chronic skin disorder often associated with immune system abnormalities. Conventional systemic therapies and biologics have limited efficacy and are prone to relapse, highlighting the urgent need for new treatment options. Upadacitinib, a highly selective JAK1 inhibitor, has shown promising results in various inflammatory skin diseases; however, its application in ACH has rarely been reported. METHODS: A retrospective analysis was conducted on 6 ACH patients treated with upadacitinib at our outpatient clinic from 2023 to 2025. Clinical data, including demographics, disease duration, sites involved, prior treatments, dosage, treatment duration, DLQI scores, and adverse events, were collected. Meanwhile, a narrative literature search was performed in PubMed, Web of Science, and Embase to identify studies on JAK inhibitors in ACH. RESULTS: Among 6 patients (2 males, 4 females; mean age 42.8 years, range 22-59; mean disease duration 1.9 years, range 6 months-4 years), all received upadacitinib 15 mg/day for 3-13 months. Most improved in skin and nail lesions; mean DLQI decreased from 14.0 to 6.0. Response was assessed primarily by clinical signs: complete response required resolution of pustules and erythema with nail improvement; partial response required >/=50% clinical improvement. One patient had clinical complete response (DLQI 71%) and another clinical partial response (DLQI 42%). No adverse events occurred. Notably, 3 patients who failed tofacitinib improved after switching, though this is preliminary and may be confounded by cumulative exposure. CONCLUSION: In this small retrospective case series, upadacitinib 15 mg/day appeared effective in ACH, with most patients showing clinical improvement, although two had subthreshold DLQI improvement despite clinical response. The literature review indicates that JAK inhibitors, particularly upadacitinib, show promising efficacy in ACH, but current evidence remains limited to case reports and small series. Response after switching from tofacitinib in three patients is preliminary and may be confounded by cumulative exposure; larger prospective studies are needed to confirm efficacy, safety, and the value of switching. CI - Copyright (c) 2026 Gan, Yan, Xie, Liu and Chen. FAU - Gan, Yu-Ting AU - Gan YT AD - Department of Dermatology, The First Affiliated Hospital of Chongqing College of Traditional Chinese Medicine, Chongqing, China. AD - Chongqing University of Chinese Medicine, Chongqing, China. FAU - Yan, Shi AU - Yan S AD - Department of Dermatology, The First Affiliated Hospital of Chongqing College of Traditional Chinese Medicine, Chongqing, China. FAU - Xie, Wei-Na AU - Xie WN AD - Department of Dermatology, The First Affiliated Hospital of Chongqing College of Traditional Chinese Medicine, Chongqing, China. FAU - Liu, Yi AU - Liu Y AD - Department of Dermatology, The First Affiliated Hospital of Chongqing College of Traditional Chinese Medicine, Chongqing, China. FAU - Chen, Feng-Lin AU - Chen FL AD - Department of Dermatology, The First Affiliated Hospital of Chongqing College of Traditional Chinese Medicine, Chongqing, China. LA - eng PT - Case Reports PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 0 (Heterocyclic Compounds, 3-Ring) RN - 4RA0KN46E0 (upadacitinib) RN - 0 (Janus Kinase Inhibitors) SB - IM MH - Humans MH - Female MH - Male MH - Adult MH - Middle Aged MH - *Heterocyclic Compounds, 3-Ring/therapeutic use/adverse effects MH - *Acrodermatitis/drug therapy/diagnosis MH - Retrospective Studies MH - Treatment Outcome MH - *Janus Kinase Inhibitors/therapeutic use MH - Young Adult PMC - PMC13713813 OTO - NOTNLM OT - Acrodermatitis continua of Hallopeau OT - JAK inhibitor OT - case series OT - literature review OT - tofacitinib OT - upadacitinib COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:41 PHST- 2026/06/08 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:41 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fimmu.2026.1903377 [doi] PST - epublish SO - Front Immunol. 2026 Oct 14;17:1903377. doi: 10.3389/fimmu.2026.1903377. eCollection 2026. PMID- 42953179 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2691-3704 (Electronic) IS - 2691-3704 (Linking) VI - 6 IP - 10 DP - 2026 Oct 26 TI - Electrochemical Platform for Automated Design-of-Experiment Optimization of Ni-Catalyzed Cross-Electrophile Coupling Reactions. PG - 5885-5893 LID - 10.1021/jacsau.6c01220 [doi] AB - Electrosynthetic organic reactions have advanced significantly in recent years, but their adoption for large-scale applications remains limited. Here, we report an automated experimental platform designed to facilitate translation of small-scale chemical and electrochemical redox reactions into robust larger-scale electrochemical processes. This platform, which integrates a commercial chemical synthesis workstation, peristaltic pumps, and a potentiostat, is equipped to conduct an automated sequence of batch experiments to optimize standard variables, such as stir rate and temperature, in addition to variables unique to electrosynthesis, such as applied current and total charge passed. Analysis of these variables using a design-of-experiment (DoE) approach accelerates identification of optimal reaction conditions and translation of mg-to-multigram operations. To evaluate the DoE optimization protocol and experimental platform, four Ni-catalyzed C-(sp (2))-C-(sp (3)) cross-electrophile coupling reactions were selected from the literature that were originally conducted on a 0.2-0.4 mmol scale with chemical or electrochemical reduction methods. Each reaction was successfully scaled by a factor of 30-250, delivering up to 20 g (100 mmol) of isolated product. These results show how small-scale redox reactions may be rapidly translated to scalable electrosynthetic processes. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Chen, Jieping AU - Chen J AD - Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States. RINGGOLD: 5228 FAU - Johnson, Mathew R AU - Johnson MR AD - Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States. RINGGOLD: 5228 FAU - Tan, Zhifeng AU - Tan Z AD - Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States. RINGGOLD: 5228 FAU - Myren, Tessa H T AU - Myren THT AD - Drug Substance Technologies - Synthetics, Process Development, Amgen Inc., One Amgen Center Drive, Thousand Oaks, California 91320, United States. RINGGOLD: 7129 FAU - Quasdorf, Kyle W AU - Quasdorf KW AUID- ORCID: 0000-0003-0151-6511 AD - Drug Substance Technologies - Synthetics, Process Development, Amgen Inc., One Amgen Center Drive, Thousand Oaks, California 91320, United States. RINGGOLD: 7129 FAU - Wei, Carolyn S AU - Wei CS AD - Drug Substance Technologies - Synthetics, Process Development, Amgen Inc., One Amgen Center Drive, Thousand Oaks, California 91320, United States. RINGGOLD: 7129 FAU - Stahl, Shannon S AU - Stahl SS AUID- ORCID: 0000-0002-9000-7665 AD - Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States. RINGGOLD: 5228 LA - eng PT - Journal Article DEP - 20260922 PL - United States TA - JACS Au JT - JACS Au JID - 101775714 PMC - PMC13714832 OTO - NOTNLM OT - automated electrochemistry OT - cross-electrophile coupling OT - design of experiments (DoE) OT - electrochemical platform OT - nickel catalysis EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/22 CRDT- 2026/10/29 05:41 PHST- 2026/07/30 00:00 [received] PHST- 2026/08/31 00:00 [revised] PHST- 2026/09/02 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:41 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - 10.1021/jacsau.6c01220 [doi] PST - epublish SO - JACS Au. 2026 Sep 22;6(10):5885-5893. doi: 10.1021/jacsau.6c01220. eCollection 2026 Oct 26. PMID- 42953176 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1662-5161 (Print) IS - 1662-5161 (Electronic) IS - 1662-5161 (Linking) VI - 20 DP - 2026 TI - A two-stage RAG-LLM pipeline for roadmapping neuroscience research in operational environments. PG - 1884892 LID - 10.3389/fnhum.2026.1884892 [doi] LID - 1884892 AB - INTRODUCTION: Neuroscientific research and applications in real world environments are often constrained by limited access, equipment limitations, and high costs. Consequently, robustly selecting constructs to focus effort on is a high-impact task. METHOD: This study introduces a two-stage large language model (LLM) pipeline that generates research roadmaps from existing real-world data to inform research priorities in the field, given the state of the art. Stage 1 uses retrieval-augmented generation on operational logs to identify candidate psychological, social, and neurocognitive constructs and map their co-occurrence. Stage 2 evaluates how well these constructs are represented in the literature across four dimensions that commonly inform prioritization: ecological validity, neural correlates, biosensor protocols, and brain computer interface readiness, producing an evidence gap matrix. Pipeline evaluation combined human review, deterministic computations, and human-LLM alignment analysis. RESULTS: Applying the pipeline to Mars Desert Research Station logs, Stage 1 identified 19 constructs. Stage 2 highlighted critical gaps in neuroimaging and measurement protocols, particularly for social domains, thereby identifying potential research priorities and risks. DISCUSSION: This methodology offers a generalizable starting point for synthesizing operational logs into actionable research roadmaps, but the evaluation showed it still required human scrutiny. CI - Copyright (c) 2026 Spencer. FAU - Spencer, Cara A AU - Spencer CA AD - Computer Science Department, University of Colorado Boulder, Boulder, CO, United States. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Hum Neurosci JT - Frontiers in human neuroscience JID - 101477954 PMC - PMC13713852 OTO - NOTNLM OT - BCI-brain computer interface OT - LLM OT - biosensor OT - ecological validity OT - neuroimaging OT - retrieval-augmented generation (RAG) COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:41 PHST- 2026/05/18 00:00 [received] PHST- 2026/08/24 00:00 [revised] PHST- 2026/09/03 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:41 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fnhum.2026.1884892 [doi] PST - epublish SO - Front Hum Neurosci. 2026 Oct 14;20:1884892. doi: 10.3389/fnhum.2026.1884892. eCollection 2026. PMID- 42953173 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2691-3704 (Electronic) IS - 2691-3704 (Linking) VI - 6 IP - 10 DP - 2026 Oct 26 TI - Modular Triethylene Glycol Decouples Antimicrobial Potency from Cytotoxicity in Membrane-Active Drugs. PG - 5949-5960 LID - 10.1021/jacsau.6c01288 [doi] AB - Cationic membrane-active drugs are an important focus of antibiotic research, yet their potential is often limited by poor selectivity. Here, we establish steric protection of cations by modular triethylene glycol as a chemical design framework to decouple antimicrobial potency from cytotoxicity. Using antipsychotics, a class of widely prescribed membrane-active drugs, we show that lipophilicity and rigidity jointly govern antimicrobial potency and mammalian cell cytotoxicity. Membrane perturbation emerged as a key determinant of bioactivity and revealed physicochemical windows that decouple antimicrobial potency and cytotoxicity. Our findings spotlight octanol as a design motif for steric protection of cations that emerged from systematic tuning of side chain lipophilicity and mimics a known metabolite motif. As exemplified with the model drug chlorpromazine, this modification can maintain water solubility, improve membrane engagement, reduce mammalian cell cytotoxicity, enhance antibacterial potency, and amplify polymyxin amplification across Gram-negative bacteria, including drug-resistant clinical isolates. Our findings indicate that membrane remodeling associated with polymyxin resistance in Escherichia coli correlates with a collateral vulnerability to cationic amphiphiles, suggesting a physicochemically tractable consequence of resistance. These findings establish a chemical design framework for small-molecule cationic amphiphiles with improved physicochemical properties, membrane engagement, and bioactivity that can be difficult to achieve by traditional alkylation. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Seewald, Marc AU - Seewald M AD - Department of Chemistry and Chemical Biology, Technical University Dortmund, Dortmund 44227, Germany. RINGGOLD: 14311 AD - Dortmund Center for Advanced Exploration of Dynamics across Limits Using Spectroscopy, Technical University Dortmund, Dortmund 44227, Germany. FAU - Stamen, Marla AU - Stamen M AD - Department of Chemistry and Chemical Biology, Technical University Dortmund, Dortmund 44227, Germany. RINGGOLD: 14311 AD - Dortmund Center for Advanced Exploration of Dynamics across Limits Using Spectroscopy, Technical University Dortmund, Dortmund 44227, Germany. FAU - Li, Jian AU - Li J AD - Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund 44227, Germany. FAU - Engelkamp, Sven AU - Engelkamp S AD - Department of Chemistry and Chemical Biology, Technical University Dortmund, Dortmund 44227, Germany. RINGGOLD: 14311 AD - Dortmund Center for Advanced Exploration of Dynamics across Limits Using Spectroscopy, Technical University Dortmund, Dortmund 44227, Germany. FAU - Behnke, Jan-Simon AU - Behnke JS AD - Department of Chemistry and Chemical Biology, Technical University Dortmund, Dortmund 44227, Germany. RINGGOLD: 14311 AD - Dortmund Center for Advanced Exploration of Dynamics across Limits Using Spectroscopy, Technical University Dortmund, Dortmund 44227, Germany. FAU - Lucassen, Ralf AU - Lucassen R AD - Faculty Life Sciences, Rhine-Waal University of Applied Sciences, Kleve 47533, Germany. FAU - Borowick, Katharina AU - Borowick K AD - Department of Chemistry and Chemical Biology, Technical University Dortmund, Dortmund 44227, Germany. RINGGOLD: 14311 AD - Dortmund Center for Advanced Exploration of Dynamics across Limits Using Spectroscopy, Technical University Dortmund, Dortmund 44227, Germany. FAU - Weilburg, Marie AU - Weilburg M AD - Department of Chemistry and Chemical Biology, Technical University Dortmund, Dortmund 44227, Germany. RINGGOLD: 14311 AD - Dortmund Center for Advanced Exploration of Dynamics across Limits Using Spectroscopy, Technical University Dortmund, Dortmund 44227, Germany. FAU - Wycisk, Virginia AU - Wycisk V AD - Department of Chemistry and Chemical Biology, Technical University Dortmund, Dortmund 44227, Germany. RINGGOLD: 14311 AD - Dortmund Center for Advanced Exploration of Dynamics across Limits Using Spectroscopy, Technical University Dortmund, Dortmund 44227, Germany. FAU - Fielitz, Tom AU - Fielitz T AD - Institute of Chemistry, University of Potsdam, Potsdam 14476, Germany. RINGGOLD: 26583 FAU - Wu, Tzu-Chien AU - Wu TC AD - Institute of Chemistry, University of Potsdam, Potsdam 14476, Germany. RINGGOLD: 26583 FAU - Hartlieb, Matthias AU - Hartlieb M AD - Institute of Chemistry, University of Potsdam, Potsdam 14476, Germany. RINGGOLD: 26583 FAU - Schaumburg, Frieder AU - Schaumburg F AD - Institute of Medical Microbiology, University Hospital Munster, Munster 48149, Germany. FAU - Bockmuhl, Dirk AU - Bockmuhl D AD - Faculty Life Sciences, Rhine-Waal University of Applied Sciences, Kleve 47533, Germany. FAU - Raisch, Tobias AU - Raisch T AUID- ORCID: 0000-0002-6531-3812 AD - Department of Structural Biochemistry, Max Planck Institute of Molecular Physiology, Dortmund 44227, Germany. FAU - Urner, Leonhard Hagen AU - Urner LH AUID- ORCID: 0000-0001-8144-8955 AD - Department of Chemistry and Chemical Biology, Technical University Dortmund, Dortmund 44227, Germany. RINGGOLD: 14311 AD - Dortmund Center for Advanced Exploration of Dynamics across Limits Using Spectroscopy, Technical University Dortmund, Dortmund 44227, Germany. LA - eng PT - Journal Article DEP - 20261011 PL - United States TA - JACS Au JT - JACS Au JID - 101775714 PMC - PMC13714845 OTO - NOTNLM OT - Cationic Amphiphile OT - Cytotoxicity OT - Escherichia coli OT - Polymyxin Resistance OT - beta-Lactam Resistance EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/11 CRDT- 2026/10/29 05:40 PHST- 2026/08/10 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:40 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - 10.1021/jacsau.6c01288 [doi] PST - epublish SO - JACS Au. 2026 Oct 11;6(10):5949-5960. doi: 10.1021/jacsau.6c01288. eCollection 2026 Oct 26. PMID- 42953164 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2691-3704 (Electronic) IS - 2691-3704 (Linking) VI - 6 IP - 10 DP - 2026 Oct 26 TI - Ground-State Charge Generation-Enabled Nondestructive Defect Passivation in Organic Photovoltaics. PG - 5816-5825 LID - 10.1021/jacsau.6c01120 [doi] AB - Ground-state charge generation (GSCG) offers a potent yet underexplored avenue for trap engineering in organic solar cells (OSCs), provided it can be harnessed without disrupting the bulk heterojunction morphology. Herein, we demonstrate an efficient GSCG strategy via FeCl(3) doping in the polymer donor D18, achieving a paradigm of "nondestructive defect passivation." Mechanistically, the GSCG-induced trap filling operates in the pre-excitation ground state, effectively neutralizing deep-level defects without compromising the donor-acceptor interfacial integrity. Ultrafast spectroscopy reveals that this ground-state premodulation selectively optimizes photophysical dynamics: while preserving efficient exciton dissociation, it profoundly suppresses nongeminate recombination and blocks the energy-loss channel associated with triplet exciton formation. Consequently, the optimized devices deliver a remarkable open-circuit voltage of 0.917 V and a photoelectric conversion efficiency of 20.67%. This work elucidates the mechanism of morphology-compatible defect passivation, establishing GSCG as a critical lever for minimizing energy loss in high-performance OSCs. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Ran, Guangliu AU - Ran G AD - School of Physics and Astronomy, Applied Optics Beijing Area Major Laboratory, Center for Advanced Quantum Studies, Beijing Normal University, and Key Laboratory of Multiscale Spin Physics (Beijing Normal University), Ministry of Education, Beijing 100875, China. FAU - Xie, Yan AU - Xie Y AD - School of Physics and Astronomy, Applied Optics Beijing Area Major Laboratory, Center for Advanced Quantum Studies, Beijing Normal University, and Key Laboratory of Multiscale Spin Physics (Beijing Normal University), Ministry of Education, Beijing 100875, China. FAU - Zhuang, Bo AU - Zhuang B AUID- ORCID: 0000-0002-9906-1190 AD - Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China. RINGGOLD: 12465 FAU - Zhang, Jiahe AU - Zhang J AD - Beijing Key Laboratory of Energy Conversion and Storage Materials College of Chemistry, Beijing Normal University, Beijing 100875, China. FAU - Lv, Tanjin AU - Lv T AD - School of Physics and Astronomy, Applied Optics Beijing Area Major Laboratory, Center for Advanced Quantum Studies, Beijing Normal University, and Key Laboratory of Multiscale Spin Physics (Beijing Normal University), Ministry of Education, Beijing 100875, China. FAU - Bo, Zhishan AU - Bo Z AUID- ORCID: 0000-0003-0126-7957 AD - Beijing Key Laboratory of Energy Conversion and Storage Materials College of Chemistry, Beijing Normal University, Beijing 100875, China. FAU - Lu, Hao AU - Lu H AUID- ORCID: 0000-0002-0559-9417 AD - College of Materials Science and Engineering, Qingdao University, Qingdao, Shandong 266071, China. RINGGOLD: 12593 FAU - Zhang, Wenkai AU - Zhang W AUID- ORCID: 0000-0002-9574-6272 AD - School of Physics and Astronomy, Applied Optics Beijing Area Major Laboratory, Center for Advanced Quantum Studies, Beijing Normal University, and Key Laboratory of Multiscale Spin Physics (Beijing Normal University), Ministry of Education, Beijing 100875, China. LA - eng PT - Journal Article DEP - 20260925 PL - United States TA - JACS Au JT - JACS Au JID - 101775714 PMC - PMC13714824 OTO - NOTNLM OT - charge transfer OT - defect passivation OT - ground-state charge generation OT - organic solar cells OT - transient absorption EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/25 CRDT- 2026/10/29 05:40 PHST- 2026/07/15 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:40 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - 10.1021/jacsau.6c01120 [doi] PST - epublish SO - JACS Au. 2026 Sep 25;6(10):5816-5825. doi: 10.1021/jacsau.6c01120. eCollection 2026 Oct 26. PMID- 42953162 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 17 DP - 2026 TI - Alopecia areata coexisting with Hashimoto's thyroiditis and Sjogren's syndrome: case report of hair regrowth and selected laboratory changes during combination treatment including tofacitinib. PG - 1967270 LID - 10.3389/fimmu.2026.1967270 [doi] LID - 1967270 AB - BACKGROUND: Alopecia areata (AA) is an autoimmune disorder characterized by transient, non-scarring hair loss and preservation of the hair follicle. Hashimoto's thyroiditis (HT) and Sjogren's syndrome (SS) are common systemic autoimmune disorders that may share genetic and immunological predispositions with AA. Co-occurrence of all three conditions is rarely reported. CASE PRESENTATION: We report a 36-year-old female who presented with a 1-year history of progressive hair loss involving the occipital scalp margins, with a Severity of Alopecia Tool (SALT) score of 30 at treatment initiation (retrospectively revised to 15 on unblinded image review). She had a 10-year history of HT on stable levothyroxine replacement and a 6-month history of SS. Laboratory evaluation revealed elevated TSH (6.299 uIU/mL), markedly elevated TPO-Ab (922.7 IU/mL), positive anti-SSA antibodies. The patient received tofacitinib 5 mg twice daily, together with intralesional compound betamethasone, topical clobetasol propionate, and topical minoxidil. At 6 months, SALT score was 0; TPO-Ab was 255 IU/mL and Tg-Ab was 430 IU/mL; anti-SSA antibodies were negative; and TSH was 2.760 uIU/mL during continued levothyroxine replacement. Baseline and follow-up thyroid assays used different platforms (CLIA vs ECLIA), and the 6-month Tg-Ab value (430 IU/mL) was numerically higher than baseline (307.52 IU/mL); therefore, neither a biological increase nor decrease in thyroid autoantibodies can be confidently inferred from these numbers alone. Hair regrowth occurred under combination treatment and cannot be attributed to tofacitinib alone. No adverse events were reported during the available follow-up (6 months in clinic; 9 months by telephone). CONCLUSION: This case describes hair regrowth and selected laboratory changes observed during combination treatment including tofacitinib in a patient with AA and comorbid HT/SS. The observations are hypothesis-generating and temporal association does not establish treatment causation. CI - Copyright (c) 2026 You, Wang, Wang and Yang. FAU - You, Fangjia AU - You F AD - Beijing University of Chinese Medicine, Beijing, China. AD - Department of Dermatology, National Center for Integrative Medicine, Hair Medicine Center, China-Japan Friendship Hospital, Beijing, China. FAU - Wang, Lei AU - Wang L AD - Department of Dermatology, National Center for Integrative Medicine, Hair Medicine Center, China-Japan Friendship Hospital, Beijing, China. FAU - Wang, Ying AU - Wang Y AD - Department of Dermatology, National Center for Integrative Medicine, Hair Medicine Center, China-Japan Friendship Hospital, Beijing, China. FAU - Yang, Dingquan AU - Yang D AD - Department of Dermatology, National Center for Integrative Medicine, Hair Medicine Center, China-Japan Friendship Hospital, Beijing, China. LA - eng PT - Case Reports PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 87LA6FU830 (tofacitinib) RN - 0 (Piperidines) RN - 0 (Pyrimidines) SB - IM MH - Humans MH - Female MH - *Hashimoto Disease/drug therapy/complications/diagnosis MH - *Alopecia Areata/drug therapy/complications/diagnosis MH - *Piperidines/therapeutic use MH - *Sjogren's Syndrome/drug therapy/complications/diagnosis MH - Adult MH - *Pyrimidines/therapeutic use MH - Drug Therapy, Combination MH - Treatment Outcome MH - *Hair/growth & development/drug effects PMC - PMC13713845 OTO - NOTNLM OT - Hashimoto's thyroiditis OT - Sjogren's syndrome OT - alopecia areata OT - case report OT - tofacitinib COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:40 PHST- 2026/08/13 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:40 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fimmu.2026.1967270 [doi] PST - epublish SO - Front Immunol. 2026 Oct 14;17:1967270. doi: 10.3389/fimmu.2026.1967270. eCollection 2026. PMID- 42953153 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2691-3704 (Electronic) IS - 2691-3704 (Linking) VI - 6 IP - 10 DP - 2026 Oct 26 TI - Glycosylation Encodes Selective Galectin Recognition and Signaling by Osteopontin. PG - 5744-5756 LID - 10.1021/jacsau.6c01041 [doi] AB - Post-translational modifications critically regulate extracellular molecular recognition, yet their roles in intrinsically disordered proteins remain poorly defined. Here, we integrate glycoproteomics, NMR, and cellular assays to reveal how glycosylation shapes the interaction network of osteopontin (OPN), a highly glycosylated intrinsically disordered protein. We show that OPN displays a heterogeneous repertoire of predominantly sialylated O-glycans that drive selective lectin binding: galectin-3 preferentially binds terminal LacNAc-containing epitopes, whereas galectin-8 engages multiple sialylated and sulfated glycan motifs, promoting cross-linking of OPN. In contrast, binding to CD44 receptor is largely glycosylation-independent and is mediated primarily through the polypeptide backbone. This dual recognition mechanism enables OPN to act as a molecular scaffold that couples glycan-mediated galectin binding with CD44 receptor engagement. Consistent with this proposed mechanistic model, OPN/galectin complexes are recruited to CD44-expressing cells where glycosylated OPN suppresses galectin-8-induced apoptosis, whereas deglycosylated OPN does not. Together, our results reveal that glycosylation encodes interaction specificity and downstream signaling outcomes in an intrinsically disordered extracellular protein, establishing OPN as a paradigm for how glycans organize extracellular recognition networks. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Bermejo, Iris A AU - Bermejo IA AD - Center for Cooperative Research in Biosciences (CIC bioGUNE), Derio 48160, Spain. FAU - Balzo, Diego Del AU - Balzo DD AD - Center for Cooperative Research in Biosciences (CIC bioGUNE), Derio 48160, Spain. FAU - Azkargorta, Mikel AU - Azkargorta M AD - Center for Cooperative Research in Biosciences (CIC bioGUNE), Derio 48160, Spain. FAU - Marina, Alberto AU - Marina A AUID- ORCID: 0000-0001-7822-0503 AD - Center for Cooperative Research in Biosciences (CIC bioGUNE), Derio 48160, Spain. FAU - Delgado, Sandra AU - Delgado S AD - Center for Cooperative Research in Biosciences (CIC bioGUNE), Derio 48160, Spain. FAU - Gimeno, Ana AU - Gimeno A AUID- ORCID: 0000-0001-9668-2605 AD - Center for Cooperative Research in Biosciences (CIC bioGUNE), Derio 48160, Spain. AD - Ikerbasque, Basque Foundation for Science, Bilbao 48009, Spain. FAU - Peccati, Francesca AU - Peccati F AUID- ORCID: 0000-0002-7813-8216 AD - Center for Cooperative Research in Biosciences (CIC bioGUNE), Derio 48160, Spain. AD - Ikerbasque, Basque Foundation for Science, Bilbao 48009, Spain. FAU - Diercks, Tammo AU - Diercks T AUID- ORCID: 0000-0002-5200-0905 AD - Center for Cooperative Research in Biosciences (CIC bioGUNE), Derio 48160, Spain. FAU - Elortza, Felix AU - Elortza F AD - Center for Cooperative Research in Biosciences (CIC bioGUNE), Derio 48160, Spain. FAU - Millet, Oscar AU - Millet O AUID- ORCID: 0000-0001-8748-4105 AD - Center for Cooperative Research in Biosciences (CIC bioGUNE), Derio 48160, Spain. FAU - Arda, Ana AU - Arda A AUID- ORCID: 0000-0003-3027-7417 AD - Center for Cooperative Research in Biosciences (CIC bioGUNE), Derio 48160, Spain. AD - Ikerbasque, Basque Foundation for Science, Bilbao 48009, Spain. FAU - Mateos, Borja AU - Mateos B AD - Inorganic and Organic Chemistry Department, University of Barcelona, Marti i Franques 1-11, Barcelona 08028, Spain. FAU - Jimenez-Barbero, Jesus AU - Jimenez-Barbero J AUID- ORCID: 0000-0001-5421-8513 AD - Center for Cooperative Research in Biosciences (CIC bioGUNE), Derio 48160, Spain. AD - Ikerbasque, Basque Foundation for Science, Bilbao 48009, Spain. AD - Department of Organic & Inorganic Chemistry, Faculty of Science and Technology, University of the Basque Country, EHU, Leioa 48940, Spain. AD - Centro de Investigacion Biomedica en Red de Enfermedades Respiratorias, Madrid 28029, Spain. FAU - Unione, Luca AU - Unione L AUID- ORCID: 0000-0002-8724-8783 AD - Center for Cooperative Research in Biosciences (CIC bioGUNE), Derio 48160, Spain. AD - Ikerbasque, Basque Foundation for Science, Bilbao 48009, Spain. LA - eng PT - Journal Article DEP - 20261001 PL - United States TA - JACS Au JT - JACS Au JID - 101775714 PMC - PMC13714850 OTO - NOTNLM OT - cell signaling OT - glycosylation OT - intrinsically disordered protein OT - molecular recognition OT - osteopontin EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/01 CRDT- 2026/10/29 05:39 PHST- 2026/07/01 00:00 [received] PHST- 2026/09/01 00:00 [revised] PHST- 2026/09/02 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:39 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - 10.1021/jacsau.6c01041 [doi] PST - epublish SO - JACS Au. 2026 Oct 1;6(10):5744-5756. doi: 10.1021/jacsau.6c01041. eCollection 2026 Oct 26. PMID- 42953144 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-2565 (Electronic) IS - 2296-2565 (Linking) VI - 14 DP - 2026 TI - Urban-rural differences in refractive status and visual behaviors among preschool children aged 3-6 years in Shunyi district, Beijing: a multidimensional analysis. PG - 1928738 LID - 10.3389/fpubh.2026.1928738 [doi] LID - 1928738 AB - PURPOSE: To investigate urban-rural differences in refractive status among children aged 3-6 years in Shunyi District, Beijing, and their associations with daily visual behaviors, as well as to ascertain the relative contributions of familial risk and environmental factors in this population. METHODS: A total of 1,142 children (527 in urban areas and 615 in townships) from 11 kindergartens in Shunyi District were selected through stratified cluster random sampling. After fully paralyzing the ciliary muscle with 1% cyclopentolate, computerized refraction was performed to calculate the spherical equivalent (SE). Standardized questionnaires were used to obtain eye-use duration, eye-use distance, outdoor activity time and parental myopia history. Multifactor logistic regression and structural equation modeling (SEM) were used to analyze myopia risk factors and their path relationships. RESULTS: The overall myopia prevalence was 3.85% (3.23% in urban and 4.39% in rural areas), with no significant urban-rural difference (chi (2) = 1.308, p = 0.253). No significant associations of sex, age, or urban-rural status on myopia prevalence, visual behaviors, or outdoor time were observed. Multivariable logistic regression indicated that parental myopia was independently associated with myopia in preschool children (OR = 4.892, 95% CI: 2.675-8.954, p < 0.001). SEM showed good fit (RMSEA = 0.024, CFI = 0.978) and revealed a significant positive direct association of parental myopia with childhood myopia (standardized path coefficient beta = 0.32, p < 0.05), which was numerically stronger in urban children. The direct associations of near work load (beta = 0.08) and outdoor activity (beta = -0.07) were not statistically significant (p > 0.05). CONCLUSION: Myopia prevalence and visual behavior patterns in preschool children in Shunyi District are homogenized between urban and rural areas. In this age group, parental myopia is the only statistically significant independent predictor of childhood myopia (OR = 4.87). This study systematically examined the prominent role of familial risk in a preschool population from the same urban-rural fringe area, and proposed the hypothesis that "familial risk becomes more prominent following convergence in measured visual behaviors." Based on these findings, we propose a tiered strategy for myopia prevention and control. CI - Copyright (c) 2026 Tang and Jiang. FAU - Tang, Yong AU - Tang Y AD - Department of Ophthalmology, Beijing Shunyi Hospital, Beijing, China. FAU - Jiang, Aimin AU - Jiang A AD - Department of Ophthalmology, Beijing Shunyi Hospital, Beijing, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Public Health JT - Frontiers in public health JID - 101616579 SB - IM MH - Humans MH - Female MH - Male MH - *Rural Population/statistics & numerical data MH - *Urban Population/statistics & numerical data MH - Child, Preschool MH - Child MH - Beijing/epidemiology MH - *Myopia/epidemiology MH - Prevalence MH - Risk Factors MH - Surveys and Questionnaires PMC - PMC13713776 OTO - NOTNLM OT - myopia prevention OT - refractive error OT - structural equation model OT - urban-rural disparity OT - visual behavior COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:39 PHST- 2026/07/05 00:00 [received] PHST- 2026/09/02 00:00 [revised] PHST- 2026/09/11 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:39 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpubh.2026.1928738 [doi] PST - epublish SO - Front Public Health. 2026 Oct 14;14:1928738. doi: 10.3389/fpubh.2026.1928738. eCollection 2026. PMID- 42953143 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-2392 (Print) IS - 1664-2392 (Electronic) IS - 1664-2392 (Linking) VI - 17 DP - 2026 TI - Gonadotropin-releasing hormone antagonists versus agonists for endometriosis: a systematic review and meta-analysis of randomized controlled trials. PG - 1979544 LID - 10.3389/fendo.2026.1979544 [doi] LID - 1979544 AB - BACKGROUND: Endometriosis is a chronic estrogen-dependent inflammatory disease requiring individualized long-term hormonal management. Gonadotropin-releasing hormone agonists (GnRH-a) effectively suppress ovarian function but may induce hypoestrogenic adverse effects, whereas oral gonadotropin-releasing hormone antagonists (GnRH-ant) offer rapid, reversible suppression with reduced hormonal fluctuation. However, their comparative efficacy and safety remain unclear. METHODS: We performed a systematic review and meta-analysis of randomized controlled trials comparing GnRH-ant with leuprolide-based GnRH-a for the treatment of endometriosis, assessing symptom relief, pelvic signs, bone mineral density (BMD), quality of life, and hormonal outcomes. RESULTS: Five randomized controlled trials were included. Compared with GnRH-ant, GnRH-a yielded greater improvements in dysmenorrhea (standardized mean difference (SMD) = 0.69, 95% CI: 0.48-0.91), nonmenstrual pelvic pain (NMPP) (SMD = 0.33, 95% CI: 0.16-0.50), overall pelvic pain (OPP) (SMD = 0.25, 95% CI: 0.13-0.38), and dyspareunia (SMD = 0.14, 95% CI: 0.02-0.26). GnRH-a was also associated with better outcomes in pelvic tenderness, Douglas pouch induration scores, peak pain severity, analgesic requirements, and menstrual blood loss. Subgroup analysis suggested a more pronounced analgesic benefit of GnRH-a after 12 weeks of treatment. In contrast, GnRH-ant resulted in significantly less BMD reduction (SMD = 0.64, 95% CI: 0.39-0.89), a stronger bone-sparing effect at 24 weeks. No significant differences were observed in most quality-of-life domains. CONCLUSIONS: Leuprolide-based GnRH-a provides superior symptom control, whereas GnRH-ant offers less decline in BMD and reversible ovarian suppression, but differences among individual antagonists should be considered. Treatment selection should be individualized based on symptom severity, bone health, and fertility goals. Long-term head-to-head trials are warranted. SYSTEMATIC REVIEW REGISTRATION: https://www.crd.york.ac.uk/PROSPERO/view/CRD420261289010, identifier CRD420261289010. CI - Copyright (c) 2026 Liu, Cai, Li, Cai, Wang, Tang, Wu and Zhu. FAU - Liu, Tianmeng AU - Liu T AD - Department of Obstetrics and Gynecology, Xiamen Susong Hospital, Xiamen, Fujian, China. FAU - Cai, Yuhong AU - Cai Y AD - Women and Children's Hospital Affiliated to Xiamen University, Xiamen Maternal and Child Health Hospital, Xiamen, Fujian, China. FAU - Li, Mingyang AU - Li M AD - Graduate School of Dalian Medical University, Dalian, China. FAU - Cai, Suqing AU - Cai S AD - Department of Obstetrics and Gynecology, Xiamen Susong Hospital, Xiamen, Fujian, China. FAU - Wang, Leilei AU - Wang L AD - Department of Obstetrics and Gynecology, Xiamen Susong Hospital, Xiamen, Fujian, China. FAU - Tang, Dongmei AU - Tang D AD - Department of Obstetrics and Gynecology, Xiamen Susong Hospital, Xiamen, Fujian, China. FAU - Wu, Yiling AU - Wu Y AD - Department of Obstetrics and Gynecology, Xiamen Susong Hospital, Xiamen, Fujian, China. FAU - Zhu, Nan AU - Zhu N AD - Department of Obstetrics and Gynecology, Xiamen Susong Hospital, Xiamen, Fujian, China. LA - eng PT - Comparative Study PT - Journal Article PT - Meta-Analysis PT - Systematic Review DEP - 20261014 PL - Switzerland TA - Front Endocrinol (Lausanne) JT - Frontiers in endocrinology JID - 101555782 RN - 33515-09-2 (Gonadotropin-Releasing Hormone) RN - 0 (Hormone Antagonists) RN - EFY6W0M8TG (Leuprolide) SB - IM MH - Humans MH - Female MH - *Endometriosis/drug therapy MH - *Gonadotropin-Releasing Hormone/antagonists & inhibitors/agonists MH - Randomized Controlled Trials as Topic MH - *Hormone Antagonists/therapeutic use MH - Leuprolide/therapeutic use MH - Quality of Life MH - Bone Density/drug effects MH - Treatment Outcome MH - Pelvic Pain/drug therapy PMC - PMC13714813 OTO - NOTNLM OT - bone mineral density OT - endometriosis OT - gonadotropin-releasing hormone agonists OT - gonadotropin-releasing hormone antagonists OT - meta-analysis OT - pelvic pain COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:39 PHST- 2026/08/26 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:39 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fendo.2026.1979544 [doi] PST - epublish SO - Front Endocrinol (Lausanne). 2026 Oct 14;17:1979544. doi: 10.3389/fendo.2026.1979544. eCollection 2026. PMID- 42953132 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-2360 (Print) IS - 2296-2360 (Electronic) IS - 2296-2360 (Linking) VI - 14 DP - 2026 TI - Beyond delabeling: long-term penicillin use and avoidance following negative penicillin challenge in children. PG - 1983812 LID - 10.3389/fped.2026.1983812 [doi] LID - 1983812 AB - BACKGROUND: Penicillin allergy labels are frequently reported in children and contribute to avoidance of first-line antibiotic therapy. In appropriately selected pediatric patients, direct penicillin challenge is an increasingly used strategy for allergy evaluation and delabeling. However, whether successful delabeling translates into subsequent penicillin use in routine clinical practice remains poorly understood. PURPOSE: This study evaluated long-term antibiotic use after successful penicillin allergy delabeling in children, focusing on subsequent penicillin reuse, continued avoidance, and factors associated with subsequent reuse. METHODS: We conducted a retrospective cohort study of 305 children referred for penicillin allergy evaluation at the Hillel Yaffe Pediatric Allergy Clinic between 2019 and 2023. Patients underwent risk-stratified skin testing and/or direct oral challenge according to institutional protocol, followed by a 5-day home treatment when indicated. Long-term outcomes, including subsequent penicillin reuse and any adverse reactions following challenge, were assessed via structured telephone interviews with caregivers. RESULTS: The median age at index reaction was 4 years; reactions were predominantly nonimmediate (59.6%) and cutaneous (98.0%). Oral provocation testing was performed in 93.8% of patients and was well tolerated in 98.6%. Full tolerance, defined as negative clinic challenge and negative 5-day home treatment, was documented in 79.7% of the cohort. Among fully tolerant children with follow-up, 63.7% subsequently reused penicillin, whereas 36.3% had no documented subsequent use. No subsequent allergic reactions were reported among children who reused penicillin. Younger age and shorter time since provocation were independently associated with greater likelihood of subsequent penicillin reuse. CONCLUSION: Direct oral challenge was safe and successfully established penicillin tolerance in most children. However, successful delabeling did not consistently translate into subsequent penicillin use, highlighting a clinically important gap between diagnostic delabeling and its real-world implementation. Improved post-delabeling communication, documentation, and integration into antimicrobial stewardship may help maximize the clinical benefit of penicillin allergy delabeling. CI - (c) 2026 Schichter-Konfino, Sharter Even Tsur, Vered, Klein, Almog and Kasem. FAU - Schichter-Konfino, Vered AU - Schichter-Konfino V AD - Pediatric Allergy and Immunology Unit, Hillel Yaffe Medical Center, Hadera, Israel. AD - Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel. FAU - Sharter Even Tsur, Noam AU - Sharter Even Tsur N AD - Pediatric Department, Meir Medical Center, Kfar Saba, Israel. FAU - Vered, Nir AU - Vered N AD - Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel. AD - Pediatric Department, Hillel Yaffe Medical Center, Hadera, Israel. FAU - Klein, Adi AU - Klein A AD - Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel. AD - Pediatric Department, Hillel Yaffe Medical Center, Hadera, Israel. FAU - Almog, Meital AU - Almog M AD - Pediatric Allergy and Immunology Unit, Bnai Zion Medical Center, Haifa, Israel. FAU - Kasem, Eias AU - Kasem E AD - Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel. AD - Clalit Health Services, Sharon-Shomron County, Netanya, Israel. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Pediatr JT - Frontiers in pediatrics JID - 101615492 PMC - PMC13713809 OTO - NOTNLM OT - allergic reaction OT - antimicrobial stewardship OT - drug provocation testing OT - penicillin allergy OT - penicillin delabeling COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:38 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:38 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fped.2026.1983812 [doi] PST - epublish SO - Front Pediatr. 2026 Oct 14;14:1983812. doi: 10.3389/fped.2026.1983812. eCollection 2026. PMID- 42953128 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2673-4842 (Electronic) IS - 2673-4842 (Linking) VI - 7 DP - 2026 TI - Antimicrobial light-based interventions for management of oral infections: a mini review. PG - 1981642 LID - 10.3389/froh.2026.1981642 [doi] LID - 1981642 AB - Limitations of conventional disinfectants and rising antimicrobial resistance have accelerated the development of light-based, resistance-free interventions. This mini-review describes the mechanisms and evidence for antimicrobial photodynamic therapy (aPDT), antimicrobial blue light (aBL), antimicrobial selective photothermolysis (aSP) and photothermal therapy (PTT). Unlike antibiotics, light-based interventions avoid inducing microbial resistance or disturbing the host microflora. Interventions were evaluated across endodontics, periodontal and peri-implant disease, oral mucosal infections, and dental caries. Current literature is heavily skewed towards aPDT, leaving other interventions underexplored despite their mechanistic promise. aPDT is currently the only one with systematic reviews and meta-analyses, whereas evidence for aBL and PTT remains confined to in-vitro and preclinical studies. In periodontitis, aPDT achieved statistical significance, but clinical improvements were negligible and below meaningful thresholds, leading official guidelines from major bodies against its routine use. Clinical evidence currently supports aPDT only in oral candidiasis, where outcomes matched conventional treatments. aSP, tested only as pulsed Nd:YAG or CW diode adjuncts to debridement, was not superior to conventional methods, and selectivity has never been measured clinically. Promising directions are aBL, due to its selectivity for periodontopathogens and link to autofluorescence-based diagnosis, and PTT for its combined antimicrobial and regeneration capacity. Physical limiting factors for aPDT and aBL are shallow effective depth and oxygen dependence in anaerobic niches, and a narrow thermal safety margin for aSP and PTT. A recurring gap is lack of translation between microbial reduction and improved clinical outcomes across interventions, highlighting the need for multi-center trials based on clinically proven standardized protocols. CI - (c) 2026 Hiltch, Steier, Rupel, Ottaviani and Poli De Figueiredo. FAU - Hiltch, Gal AU - Hiltch G AD - Department of Dentistry, Federal University of Rio Grande do Sul-UFRGS, Porto Alegre, Brazil, Porto Alegre, Brazil. FAU - Steier, Liviu AU - Steier L AD - Department of Preventive and Restorative Sciences, Dental School, University of Pennsylvania-UPENN, Philadelphia, PA, United States. FAU - Rupel, Katia AU - Rupel K AD - Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Italy. FAU - Ottaviani, Giulia AU - Ottaviani G AD - Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Italy. FAU - Poli De Figueiredo, Jose Antonio AU - Poli De Figueiredo JA AD - Department of Morphological Sciences, Federal University of Rio Grande do Sul-UFRGS, Porto Alegre, Brazil. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Oral Health JT - Frontiers in oral health JID - 9918227262706676 PMC - PMC13714801 OTO - NOTNLM OT - antimicrobial blue light OT - antimicrobial resistance OT - antimicrobial therapy OT - oral infection OT - photothermal therapy (PPT) COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The authors GO, LS declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:38 PHST- 2026/08/28 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:38 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/froh.2026.1981642 [doi] PST - epublish SO - Front Oral Health. 2026 Oct 14;7:1981642. doi: 10.3389/froh.2026.1981642. eCollection 2026. PMID- 42953127 OWN - NLM STAT- Publisher LR - 20261029 IS - 2046-2069 (Electronic) IS - 2046-2069 (Linking) DP - 2026 Oct 28 TI - Synthesis of deuterated ALC-0315 for contrast variation SANS analysis of lipid nanoparticles. LID - 10.1039/d6ra04368b [doi] AB - Deuterated ALC-0315 was synthesized via two steps from three deuterated precursors. A hydrogen-deuterium (H/D) exchange protocol using a Pt/C catalyst was developed for the key precursor, 1-amino-butanol-d (8). SANS study reveals that deuteration significantly increases the contrast match point (CMP) of ALC-0315, allowing for contrast-variation small angle neutron scattering measurements to selectively highlight internal LNP core-shell structure. CI - This journal is (c) The Royal Society of Chemistry. FAU - Zhang, Hong-Hai AU - Zhang HH AUID- ORCID: 0000-0003-1413-8847 AD - Center for Nanophase Materials Sciences, Oak Ridge National Laboratory Oak Ridge Tennessee 37831 USA zhangh1@ornl.gov. FAU - Leite, Wellington AU - Leite W AD - Neutron Scattering Division, Oak Ridge National Laboratory Oak Ridge Tennessee 37831 USA. FAU - Pingali, Sai Venkatesh AU - Pingali SV AUID- ORCID: 0000-0001-7961-4176 AD - Neutron Scattering Division, Oak Ridge National Laboratory Oak Ridge Tennessee 37831 USA. FAU - Nusser, Brandon AU - Nusser B AD - Center for Nanophase Materials Sciences, Oak Ridge National Laboratory Oak Ridge Tennessee 37831 USA zhangh1@ornl.gov. FAU - Urban, Volker S AU - Urban VS AUID- ORCID: 0000-0002-7962-3408 AD - Neutron Scattering Division, Oak Ridge National Laboratory Oak Ridge Tennessee 37831 USA. FAU - O'Neill, Hugh AU - O'Neill H AD - Neutron Scattering Division, Oak Ridge National Laboratory Oak Ridge Tennessee 37831 USA. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - RSC Adv JT - RSC advances JID - 101581657 PMC - PMC13714720 COIS- There are no conflicts to declare. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 PMCR- 2026/10/28 CRDT- 2026/10/29 05:38 PHST- 2026/05/19 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:38 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - d6ra04368b [pii] AID - 10.1039/d6ra04368b [doi] PST - aheadofprint SO - RSC Adv. 2026 Oct 28. doi: 10.1039/d6ra04368b. PMID- 42953123 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2297-055X (Print) IS - 2297-055X (Electronic) IS - 2297-055X (Linking) VI - 13 DP - 2026 TI - Mechanism-defined secondary hypertension enables precision diagnosis and treatment: an evidence review. PG - 1958980 LID - 10.3389/fcvm.2026.1958980 [doi] LID - 1958980 AB - BACKGROUND: Secondary hypertension is under-recognised despite the availability of mechanism-based diagnostic tests and, for several aetiologies, potentially curative or highly targeted therapy. The clinically important question is not only which causes exist, but how reliably mechanisms translate into diagnosis and cause-directed treatment. OBJECTIVES: To systematically synthesise evidence across the principal causes of secondary hypertension using a mechanism --> diagnosis --> therapy framework; to compare diagnostic performance and cause-directed treatment effects; and to examine concordance between contemporary society guidelines and real-world implementation. METHODS: This systematic review followed Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020, with diagnostic accuracy reporting informed by PRISMA-DTA. MEDLINE (PubMed), Embase, CENTRAL, Web of Science Core Collection, Scopus, ClinicalTrials.gov, and the WHO ICTRP were searched from inception to 30 June 2025, supplemented by citation searching. The formal search identified 580 records; after removal of 168 duplicates, 412 records were screened, 106 full-text reports were assessed, and 58 included evidence records were represented across 63 publications. The review was not prospectively registered in PROSPERO. Screening, extraction and structured appraisal were performed by a single reviewer (the first author); therefore, no dual-reviewer agreement statistic was applicable. Risk of bias was appraised with RoB 2, ROBINS-I, QUADAS-2, and AMSTAR 2 as appropriate, and certainty was summarised using GRADE. A targeted post-search surveillance update through 31 July 2026 was used only to contextualise major newly available guidelines and Phase 3 trial reports and did not alter the original PRISMA counts. RESULTS: Primary aldosteronism (PA) is best understood as a continuum of renin-independent aldosterone excess: in Brown et al. [The unrecognized prevalence of primary aldosteronism: a cross-sectional study. Ann Intern Med. (2020) 173:10-20], biochemically overt autonomous aldosterone production was present in 11.3%, 15.7%, 21.6%, and 22.0% across normotensive, stage 1, stage 2, and resistant-hypertension strata, respectively. In unilateral PA, the PASO cohort distinguished complete biochemical success after adrenalectomy (94%; 656/699) from complete clinical success (37%; 259/705), with partial clinical improvement in a further 47%. PATHWAY-2 established spironolactone as the most effective fourth-line drug in resistant hypertension; CORAL, ASTRAL, STAR, and DRASTIC did not support routine revascularisation for stable atherosclerotic renal-artery stenosis; plasma-free metanephrines remained highly sensitive for phaeochromocytoma/paraganglioma; and PA testing in eligible patients with resistant hypertension remained only 1.6%-2.1%. Post-search Phase 3 trials of selective aldosterone synthase inhibition showed approximately 9 mm Hg placebo-corrected office systolic blood pressure reductions, but cardiovascular-outcome benefit has not been established, and electrolyte/renal monitoring remains necessary. CONCLUSIONS: Across secondary hypertension, a limited set of pathophysiological effector pathways maps to specific diagnostic tests and cause-directed treatments. The strongest opportunities for clinical gain are earlier recognition of high-yield phenotypes, accurate aetiological subtyping, and implementation of established testing pathways; emerging aldosterone synthase inhibitors expand blood-pressure-lowering options but should not yet be interpreted as proven cardiovascular-outcome therapies. CI - (c) 2026 Choudhary, Hait and Baladaniya. FAU - Choudhary, Arbind Kumar AU - Choudhary AK AD - Department of Pharmacology, Government Erode Medical College and Hospital, Perundurai, Tamil Nadu, India. FAU - Hait, Anamitra AU - Hait A AD - Department of Internal Medicine, Eastern Railway Production Unit Hospital, Chittaranjan, West Bengal, India. FAU - Baladaniya, Maheshkumar AU - Baladaniya M AD - Department of Physical Therapy, Neighborhood PT PC, New City, NY, United States. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Cardiovasc Med JT - Frontiers in cardiovascular medicine JID - 101653388 PMC - PMC13714811 OTO - NOTNLM OT - endocrine hypertension OT - molecular diagnostics OT - precision medicine OT - primary aldosteronism OT - renovascular hypertension OT - resistant hypertension OT - secondary hypertension OT - targeted therapy COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:37 PHST- 2026/08/05 00:00 [received] PHST- 2026/08/28 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:37 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fcvm.2026.1958980 [doi] PST - epublish SO - Front Cardiovasc Med. 2026 Oct 14;13:1958980. doi: 10.3389/fcvm.2026.1958980. eCollection 2026. PMID- 42953121 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 17 DP - 2026 TI - Cytoskeletal and phagolysosomal failure in macrophage antibacterial immunity: the PRRSV-porcine alveolar macrophage axis as a natural-host model. PG - 1892313 LID - 10.3389/fimmu.2026.1892313 [doi] LID - 1892313 AB - Viral infection of professional phagocytes can compromise antibacterial immunity because the cellular machinery reshaped for viral replication and immune evasion overlaps with the machinery required for bacterial capture, intracellular killing, and inflammatory signaling. Phagocyte cell biology has defined core cytoskeletal and trafficking nodes, including Rho-family GTPase control of actin assembly, integrin-mediated adhesion, and Rab-dependent phagosome maturation, and shown how bacterial pathogens target these nodes to evade clearance. Here we ask how recently described porcine reproductive and respiratory syndrome virus (PRRSV) lesions in porcine alveolar macrophages (PAMs) fit into this established cell biology. Direct evidence supports reduced phagocytic capture and impaired intracellular bacterial killing in defined systems. In vivo coinfection studies also report worse outcomes. Enhanced association or internalization of selected bacteria has also been reported, with ITGalpha5 and filamin A upregulation proposed as a candidate explanation. PRRSV further disrupts innate-immune signaling, autophagy-lysosome trafficking, ER-mitochondrial metabolism, and lipid homeostasis. These changes may contribute to antibacterial failure, but bacterial endpoints were not measured in the same experiments. At the tissue level, macrophage attrition and altered bystander states may disrupt cytokine relay, creating a permissive lung environment even when only a minority of PAMs are productively infected. PRRSV is particularly informative because it productively infects a professional phagocyte in the natural host, allowing viral perturbation, macrophage antibacterial function, and bacterial challenge to be examined in one tractable system. Because the candidate nsp5-RhoA trafficking route and the ITGalpha5/FLNA entry route were reported in different experimental systems, their potential combination defines a testable hypothesis requiring co-validation in the same primary PAM system. We use the PRRSV-PAM axis as a natural-host model to examine how viral infection of professional phagocytes can produce macrophage antibacterial failure, with implications for virus-associated bacterial vulnerability in veterinary and human respiratory disease. CI - Copyright (c) 2026 Wang, Shang, Yu, Li, Ji, Wang, Chen and Qi. FAU - Wang, Yubo AU - Wang Y AD - Department of Clinical Laboratory, The First Affiliated Hospital of Dalian Medical University, Dalian, China. FAU - Shang, Yihao AU - Shang Y AD - Department of Clinical Laboratory, The First Affiliated Hospital of Dalian Medical University, Dalian, China. FAU - Yu, Huiyang AU - Yu H AD - Department of Comparative Medicine, Dalian Medical University, Dalian, China. FAU - Li, Xintong AU - Li X AD - Department of Comparative Medicine, Dalian Medical University, Dalian, China. FAU - Ji, Ailin AU - Ji A AD - Department of Nephrology, The First Affiliated Hospital of Dalian Medical University, Dalian, China. FAU - Wang, Liang AU - Wang L AD - Department of Comparative Medicine, Dalian Medical University, Dalian, China. FAU - Chen, Jilin AU - Chen J AD - Department of Nephrology, The First Affiliated Hospital of Dalian Medical University, Dalian, China. FAU - Qi, Yu AU - Qi Y AD - Department of Clinical Laboratory, The First Affiliated Hospital of Dalian Medical University, Dalian, China. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 SB - IM MH - Animals MH - *Macrophages, Alveolar/immunology/virology/metabolism MH - *Porcine respiratory and reproductive syndrome virus/immunology MH - Swine MH - *Phagosomes/immunology/metabolism MH - *Porcine Reproductive and Respiratory Syndrome/immunology/virology MH - Immunity, Innate MH - *Cytoskeleton/immunology/metabolism MH - Phagocytosis/immunology MH - Host-Pathogen Interactions/immunology PMC - PMC13714797 OTO - NOTNLM OT - PRRSV OT - host-pathogen interaction OT - innate immunity OT - macrophage antibacterial immunity OT - phagolysosomal killing OT - porcine alveolar macrophages OT - professional phagocytes OT - viral-bacterial coinfection COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:37 PHST- 2026/05/27 00:00 [received] PHST- 2026/08/28 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:37 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fimmu.2026.1892313 [doi] PST - epublish SO - Front Immunol. 2026 Oct 14;17:1892313. doi: 10.3389/fimmu.2026.1892313. eCollection 2026. PMID- 42953120 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2624-8212 (Electronic) IS - 2624-8212 (Linking) VI - 9 DP - 2026 TI - Segmentation of ovarian cancer tumors from CT images based on a two-stage neural network fusion system. PG - 1974931 LID - 10.3389/frai.2026.1974931 [doi] LID - 1974931 AB - The detection of ovarian cancer using computed tomography images is making rapid progress, combining the strengths of different neural networks within a complex ensemble, a more effective model can be created to support medical decision-making in ovarian cancer cases. Various neural networks and ensemble models were tested on the TCGA OV database. Tumor regions were segmented using computer-assisted annotation masks, while molecular subtype labels were assigned at the patient level based on TCGA genomic classifications and mapped to tumors. As the main novelty, the proposed method involves a two-stage fusion system. The first stage combines several neural networks into four ensembles to capture diverse features, and the second stage integrates these results into a global system to enhance segmentation accuracy. The selected models demonstrated strong segmentation performance within the investigated experimental setting and were subsequently integrated into the proposed two-stage fusion framework. The neural networks for the first stage are Attention U-Net, DeepLabV3+, and ViT. The results show that the proposed two-stage fusion system achieved higher observed segmentation performance than the evaluated individual neural networks and first-stage ensemble configurations within the present TCGA-OV experimental setting. Across the five evaluated classes, the Global System achieved an average F1 score of 93.53%, recall of 93.38%, and precision of 93.68%, supporting the technical feasibility of the proposed two-stage fusion approach for ovarian tumor segmentation within the investigated dataset. CI - Copyright (c) 2026 Andone, Ichim and Popescu. FAU - Andone, Miruna-Cristina AU - Andone MC AD - Faculty of Automatic Control and Computers, National University of Science and Technology Politehnica of Bucharest, Bucharest, Romania. FAU - Ichim, Loretta AU - Ichim L AD - Faculty of Automatic Control and Computers, National University of Science and Technology Politehnica of Bucharest, Bucharest, Romania. AD - Stefan S. Nicolau Institute of Virology, Romanian Academy, Bucharest, Romania. FAU - Popescu, Dan AU - Popescu D AD - Faculty of Automatic Control and Computers, National University of Science and Technology Politehnica of Bucharest, Bucharest, Romania. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Artif Intell JT - Frontiers in artificial intelligence JID - 101770551 PMC - PMC13713784 OTO - NOTNLM OT - computed tomography OT - ensemble strategies OT - fusion decision OT - neural networks OT - ovarian cancer OT - tumor segmentation COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:37 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:37 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/frai.2026.1974931 [doi] PST - epublish SO - Front Artif Intell. 2026 Oct 14;9:1974931. doi: 10.3389/frai.2026.1974931. eCollection 2026. PMID- 42953117 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2045-7758 (Print) IS - 2045-7758 (Electronic) IS - 2045-7758 (Linking) VI - 16 IP - 11 DP - 2026 Nov TI - Lights Out in the Canopy: Arboreal Camera Traps Offer New Insights Into Sleeping-Site Partitioning and Sleep Ecology in Primate Communities. PG - e74525 LID - 10.1002/ece3.74525 [doi] LID - e74525 AB - Sleep is an essential yet costly biological process, leaving animals vulnerable to predation and constraining their use of space and time. Optimal sleeping sites therefore represent valuable, potentially contested resources, yet they remain largely overlooked in primate community ecology. Understanding how syntopic species compete for and partition sleeping sites can reveal mechanisms of coexistence, but community-level studies remain limited because multiple arboreal species are difficult to monitor simultaneously. Here, we used arboreal camera traps to investigate sleeping-site use and spatiotemporal partitioning within a Brazilian Atlantic Forest primate community. Over 7 months (2024-2025), we monitored ten Copaifera trapezifolia trees used as preferred sleeping sites by southern muriquis (Brachyteles arachnoides), brown howler monkeys (Alouatta guariba) and black-tufted capuchins (Sapajus nigritus cucullatus), recording 4195 videos documenting 1253 visits and 158 sleeping nights. C. trapezifolia trees constituted a shared but limited resource, and access reflected predicted interspecific differences in resource holding potential (RHP), the relative ability of a species to prevail in direct contests over a shared resource. Muriquis and capuchins, the higher-RHP species with larger home ranges, showed high temporal overlap in copaiba use-preferentially sleeping there on warmer nights and under lower lunar illumination, suggesting roles for thermoregulation and perceived predation risk-offset by reduced spatial overlap, consistent with greater ranging flexibility enabling spatial avoidance. Howlers, constrained by more restricted ranging and repeatedly displaced in direct encounters with the other species, instead relied on temporal segregation to access the same preferred trees. Nocturnal videos further revealed activity beyond residual wakefulness at the edges of the night, offering rare direct evidence of nocturnal behaviour and fragmented sleep in wild arboreal primates. These findings indicate that sleeping sites can function as contested, high-value resources structuring primate community dynamics through asymmetric contest competition, and highlight arboreal camera trapping as a powerful tool for studying primate sleep ecology. CI - (c) 2026 The Author(s). Ecology and Evolution published by British Ecological Society and John Wiley & Sons Ltd. FAU - Kaisin, Olivier AU - Kaisin O AUID- ORCID: 0000-0002-4826-131X AD - Laboratorio de Primatologia, Departamento de Biodiversidade, Instituto de Biociencias Universidade Estadual Paulista (UNESP) Rio Claro Sao Paulo Brazil. AD - Gembloux Agro-Bio Tech, Forest Is Life - TERRA Teaching and Research Center University of Liege (ULiege) Gembloux Belgium. FAU - Pochez, Matheo AU - Pochez M AD - Laboratorio de Primatologia, Departamento de Biodiversidade, Instituto de Biociencias Universidade Estadual Paulista (UNESP) Rio Claro Sao Paulo Brazil. AD - Gembloux Agro-Bio Tech, Forest Is Life - TERRA Teaching and Research Center University of Liege (ULiege) Gembloux Belgium. FAU - Vermeulen, Cedric AU - Vermeulen C AUID- ORCID: 0000-0002-1618-1892 AD - Gembloux Agro-Bio Tech, Forest Is Life - TERRA Teaching and Research Center University of Liege (ULiege) Gembloux Belgium. FAU - Culot, Laurence AU - Culot L AUID- ORCID: 0000-0002-3353-0134 AD - Laboratorio de Primatologia, Departamento de Biodiversidade, Instituto de Biociencias Universidade Estadual Paulista (UNESP) Rio Claro Sao Paulo Brazil. LA - eng SI - Dryad/10.5061/dryad.j3tx95xws PT - Journal Article DEP - 20261028 PL - England TA - Ecol Evol JT - Ecology and evolution JID - 101566408 PMC - PMC13714698 OTO - NOTNLM OT - Atlantic Forest OT - arboreal camera trapping OT - coexistence OT - sleep ecology OT - sleeping-site selection OT - spatiotemporal overlap COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/28 CRDT- 2026/10/29 05:37 PHST- 2026/06/22 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:37 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - ECE374525 [pii] AID - 10.1002/ece3.74525 [doi] PST - epublish SO - Ecol Evol. 2026 Oct 28;16(11):e74525. doi: 10.1002/ece3.74525. eCollection 2026 Nov. PMID- 42953112 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Whole-body volatile organic compound profiling reveals metabolic pathway perturbations in a murine polymicrobial sepsis model: a feasibility study. PG - 1978491 LID - 10.3389/fmed.2026.1978491 [doi] LID - 1978491 AB - Sepsis rewires host metabolism, and these changes may be readable in the volatile organic compounds (VOCs) released from the body. Whether whole-body VOC profiles can be mapped onto specific disrupted biochemical pathways has not been tested. In this discovery-phase, single-time-point feasibility study, polymicrobial sepsis was induced by cecal ligation and puncture (CLP) in C57BL/6 mice (14 CLP, 10 sham controls). Whole-body headspace gases were collected 12 h after surgery and analysed by thermal desorption-gas chromatography-mass spectrometry. After preprocessing, 113 features were tested univariately with Benjamini-Hochberg correction and by orthogonal partial least squares discriminant analysis (OPLS-DA). Candidate VOCs (FDR < 0.1, |log2FC| > 0.26, VIP > 1.0) were annotated against PubChem, KEGG COMPOUND, and the Human Metabolome Database. OPLS-DA separated the groups (R2X = 0.489, R2Y = 0.981, Q2 = 0.777; P = 0.001 by 1,000 permutations), and leave-one-out cross-validation classified 95.8% of animals correctly, though n = 24. Of 54 candidate VOCs, 18 had established endogenous origins; 16 fell and 2 rose in septic mice. Pathway mapping indicated four perturbed domains: lipid peroxidation (12 depleted VOCs), ketone body metabolism (3 decreased), cholesterol/isoprenoid metabolism (isoprene, decreased), and putative gut microbiota-associated metabolism (toluene and carbon disulfide, increased). All 18 endogenous VOCs kept candidate status after environment-derived VOCs were removed. Because whole-body headspace volatiles superimpose tissue metabolism, ventilation, activity, and background, these results suggest the septic volatilome may be mapped onto distinct pathway perturbations; ventilation and activity effects cannot be excluded. These findings generate testable hypotheses for non-invasive metabolic phenotyping in sepsis. CI - (c) 2026 Feng, Chen, Zhang, Fu, Hu, Mo and Wang. FAU - Feng, Wan-Yi AU - Feng WY AD - Department of Anesthesiology, Changhai Hospital, Naval Medical University, Shanghai, China. FAU - Chen, Yu AU - Chen Y AD - Department of Anesthesiology, Changhai Hospital, Naval Medical University, Shanghai, China. FAU - Zhang, Xiu-Ya AU - Zhang XY AD - Department of Naval Nutrition and Food Hygiene, Faculty of Naval Medicine, Naval Medical University, Shanghai, China. AD - Key Laboratory of Biological Defense, Ministry of Education, Naval Medical University, Shanghai, China. FAU - Fu, Rong AU - Fu R AD - Department of Naval Nutrition and Food Hygiene, Faculty of Naval Medicine, Naval Medical University, Shanghai, China. AD - Key Laboratory of Biological Defense, Ministry of Education, Naval Medical University, Shanghai, China. FAU - Hu, Qin AU - Hu Q AD - Department of Anesthesiology, Changhai Hospital, Naval Medical University, Shanghai, China. FAU - Mo, Feng-Feng AU - Mo FF AD - Department of Naval Nutrition and Food Hygiene, Faculty of Naval Medicine, Naval Medical University, Shanghai, China. AD - Key Laboratory of Biological Defense, Ministry of Education, Naval Medical University, Shanghai, China. FAU - Wang, Jia-Feng AU - Wang JF AD - Department of Anesthesiology, Changhai Hospital, Naval Medical University, Shanghai, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13713802 OTO - NOTNLM OT - cecal ligation and puncture OT - gas chromatography-mass spectrometry OT - gut microbiome OT - immunometabolism OT - lipid peroxidation OT - sepsis OT - volatile organic compounds OT - whole-body headspace volatilomics COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers at the time of submission. This had no impact on the peer review process and the final decision. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:37 PHST- 2026/08/25 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:37 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmed.2026.1978491 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 14;13:1978491. doi: 10.3389/fmed.2026.1978491. eCollection 2026. PMID- 42953104 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Association between preserved ratio impaired spirometry and postoperative pulmonary complications in patients undergoing lung cancer resection. PG - 1803143 LID - 10.3389/fmed.2026.1803143 [doi] LID - 1803143 AB - BACKGROUND: Postoperative pulmonary complications (PPCs) are associated with adverse clinical outcomes and increased hospitalization-related costs. Preserved ratio impaired lung function (PRISm) is a lung function phenotype distinct from obstructive lung disease, and little is known about the effect of PRISm on PPCs. The aim of this study was to investigate the association between PRISm and PPCs in patients undergoing lung cancer resection. METHODS: This retrospective cohort study involved patients who underwent lung cancer resection in the thoracic surgery case database between January 2019 and June 2019 at Shanghai Pulmonary Hospital. Demographics, preoperative measurements of spirometry, operative characteristics, PPCs, and length of stay were assessed. RESULTS: The incidence of PPCs in the three groups was 36.84% in the PRISm group, 23.39% in the normal spirometry group, and 51.57% in the COPD group. Using a variable-selection strategy (forced clinical confounders plus univariable screening at P < 0.20), patients with PRISm were 2.07 times more likely to develop a PPC compared with normal spirometry [95% confidence interval (95% CI): 1.21-3.55; p = 0.008], and the relationship was not statistically different between PRISm and COPD groups [odds ratio (OR) = 0.63, 95% CI: 0.38-1.04; p = 0.071]. In subgroup analyses, non-restrictive PRISm was not associated with a significantly increased risk of PPCs relative to restrictive PRISm (adjusted OR = 1.32, 95% CI: 0.59-2.95; p = 0.494). CONCLUSIONS: Compared with normal spirometry, PRISm was independently associated with a higher risk of PPCs in patients undergoing lung cancer resection. Preoperative identification of PRISm may refine preoperative risk stratification. Candidate targeted strategies, such as preoperative pulmonary rehabilitation, lung-protective intraoperative ventilation, and intensified postoperative surveillance, warrant prospective evaluation. The risk did not differ significantly between PRISm subtypes, though this finding requires further validation. CI - (c) 2026 Zhou, Sun, Zhang, Xu, Zhu, He, Liu and Guo. FAU - Zhou, Tianran AU - Zhou T AD - Department of Pulmonary Function Test, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China. FAU - Sun, Xingxing AU - Sun X AD - Department of Pulmonary Function Test, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China. FAU - Zhang, Jiao AU - Zhang J AD - Department of Pulmonary Function Test, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China. FAU - Xu, Jianhua AU - Xu J AD - Department of Pulmonary Function Test, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China. FAU - Zhu, Hanqing AU - Zhu H AD - Department of Pulmonary Function Test, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China. FAU - He, Mian AU - He M AD - Department of Pulmonary Function Test, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China. FAU - Liu, Jinming AU - Liu J AD - Department of Cardio-Pulmonary Circulation, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China. FAU - Guo, Jian AU - Guo J AD - Department of Pulmonary Function Test, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13713789 OTO - NOTNLM OT - COPD OT - postoperative pulmonary complication OT - postoperative pulmonary complications OT - preserved ratio impaired spirometry OT - spirometry COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:36 PHST- 2026/02/03 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:36 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmed.2026.1803143 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 14;13:1803143. doi: 10.3389/fmed.2026.1803143. eCollection 2026. PMID- 42953098 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-4185 (Print) IS - 2296-4185 (Electronic) IS - 2296-4185 (Linking) VI - 14 DP - 2026 TI - Synthetic genome shuffling of poxviruses in yeast generates novel poxvirus candidates with potential oncolytic properties. PG - 1943548 LID - 10.3389/fbioe.2026.1943548 [doi] LID - 1943548 AB - Oncolytic viruses represent a promising class of biotherapeutics, yet their clinical performance is often constrained by limited cytolytic potency and insufficient immune activation. To address these limitations, we developed a synthetic biology framework that applies genome-scale recombination in yeast to generate chimeric poxviruses derived from multiple non-human-pathogenic poxvirus lineages. We first established the near-complete assembly of a vaccinia virus genome within yeast and demonstrated its ability to yield a functional virus in human cancer cells, providing a modular foundation for genome engineering. Building on this platform, we combined genomic inputs from different poxvirus species to create a diverse library of chimeric constructs, a subset of which could be recovered as infectious particles. Phenotypic characterization revealed substantial variation among chimeras, including distinct plaque morphologies, spreading behaviors, and activity across human cancer cell lines, indicating the emergence of functional diversity rather than incremental modification. Whole-genome sequencing confirmed that individual chimeras integrated genetic segments from several parental species, validating the broad recombination capacity of the yeast-based system. Collectively, these results establish a conceptual advance in synthetic virology. Yeast-mediated genome shuffling enables the rapid generation of functionally diverse chimeric poxviruses from multispecies inputs, providing a flexible and scalable platform for the identification and development of future oncolytic poxvirus candidates. While the chimeric viruses described here do not yet contain the attenuating mutations required for tumor-selective replication, they provide a foundation for the engineering of next-generation oncolytic biotherapeutics. CI - Copyright (c) 2026 Agaoua, Rey, Hortelano, Moro, Grellier and Erbs. FAU - Agaoua, Aimeric AU - Agaoua A AD - Transgene, Illkirch-Graffenstaden, France. FAU - Rey, Camille AU - Rey C AD - Transgene, Illkirch-Graffenstaden, France. FAU - Hortelano, Julie AU - Hortelano J AD - Transgene, Illkirch-Graffenstaden, France. FAU - Moro, Anne-Isabelle AU - Moro AI AD - Transgene, Illkirch-Graffenstaden, France. FAU - Grellier, Benoit AU - Grellier B AD - Transgene, Illkirch-Graffenstaden, France. FAU - Erbs, Philippe AU - Erbs P AD - Transgene, Illkirch-Graffenstaden, France. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Bioeng Biotechnol JT - Frontiers in bioengineering and biotechnology JID - 101632513 PMC - PMC13713799 OTO - NOTNLM OT - oncolytic OT - poxvirus OT - shuffling OT - synthetic OT - tar OT - viruses COIS- All authors were employees and shareholders of Transgene. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:35 PHST- 2026/07/20 00:00 [received] PHST- 2026/09/10 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:35 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1943548 [pii] AID - 10.3389/fbioe.2026.1943548 [doi] PST - epublish SO - Front Bioeng Biotechnol. 2026 Oct 14;14:1943548. doi: 10.3389/fbioe.2026.1943548. eCollection 2026. PMID- 42953096 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2624-9367 (Electronic) IS - 2624-9367 (Linking) VI - 8 DP - 2026 TI - Sport-related neurocognitive performance and repetitive head-impact exposure in elite Muay Thai athletes: a matched athletic-control study. PG - 1908733 LID - 10.3389/fspor.2026.1908733 [doi] LID - 1908733 AB - BACKGROUND: Repetitive head impacts (RHIs) are increasingly studied in combat sports, but evidence regarding neurocognitive performance in elite Muay Thai athletes remains limited. This study compared neurocognitive performance between elite Muay Thai athletes and sprint-athlete controls and explored associations between cumulative RHI exposure and neurocognitive outcomes within the Muay Thai cohort. METHODS: This cross-sectional athletic-control study included 44 elite male Muay Thai athletes and 42 male sprint-athlete controls aged 15-25 years. Controls were selected for group-level comparability in age, education, training years, and competitive level. Neurocognitive performance was assessed using the Rey Auditory Verbal Learning Test, Go/No-Go task, Stroop Colour-Word Test, Trail Making Test Parts A and B, Digit Span, and Symbol Digit Modalities Test. Task-specific outcomes and standardised domain-level indicators were examined using independent-group comparisons and covariate-adjusted ANCOVA models. Exploratory Spearman correlations examined associations between a cumulative RHI exposure score derived from self-reported proxy indicators and neurocognitive outcomes within the Muay Thai cohort. RESULTS: No significant between-group differences were observed in task-specific or domain-level neurocognitive outcomes (all p > 0.05), and adjusted ANCOVA models showed no significant group effects across the four domain-level indicators. Within the Muay Thai cohort, cumulative RHI exposure was associated with the executive-function composite (rho = -0.32, p = 0.036), TMT-A completion time (rho = -0.44, p = 0.003), TMT-B completion time (rho = -0.41, p = 0.006), and Stroop interference time (rho = 0.56, p < 0.001). No other associations were significant. CONCLUSIONS: Elite Muay Thai athletes did not demonstrate significant group-level neurocognitive differences compared with sprint-athlete controls. Exploratory associations between cumulative RHI exposure and selected neurocognitive outcomes were observed within the Muay Thai cohort but were not consistent across the broader neurocognitive battery. These findings should not be interpreted as evidence of generalised neurocognitive impairment. Given the cross-sectional design, retrospective proxy-based exposure assessment, and exploratory correlations unadjusted for multiple comparisons, the findings are hypothesis-generating rather than causal or confirmatory. Prospective longitudinal studies incorporating objective head-impact monitoring and repeated neurocognitive assessment are needed. CI - (c) 2026 Tengku Sulaiman. FAU - Tengku Sulaiman, Raja Syed AU - Tengku Sulaiman RS AD - Major in Physical Education, School of Education, Walailak University, Nakhon Si Thammarat, Thailand. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Sports Act Living JT - Frontiers in sports and active living JID - 101765780 PMC - PMC13713806 OTO - NOTNLM OT - Muay Thai OT - combat sports OT - executive function OT - repetitive head impacts OT - sport-related neurocognitive performance COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:35 PHST- 2026/06/16 00:00 [received] PHST- 2026/08/28 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:35 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fspor.2026.1908733 [doi] PST - epublish SO - Front Sports Act Living. 2026 Oct 14;8:1908733. doi: 10.3389/fspor.2026.1908733. eCollection 2026. PMID- 42953080 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-1078 (Print) IS - 1664-1078 (Electronic) IS - 1664-1078 (Linking) VI - 17 DP - 2026 TI - Value orientations in large-scale English language assessments: a psychological analysis based on Schwartz's theory and stakeholder perceptions. PG - 1820640 LID - 10.3389/fpsyg.2026.1820640 [doi] LID - 1820640 AB - Large-scale language assessments are commonly viewed as instruments for measuring linguistic proficiency. However, from a Critical Language Testing perspective, they may also be understood as institutional contexts in which socially and culturally meaningful content is selected and represented. Drawing on Schwartz's Basic Human Values Theory as a theoretically grounded descriptive taxonomy, this study examines how value orientations are represented and distributed in large-scale English language assessment materials in China, and how they are perceived by student stakeholders. The corpus comprised 149 value-oriented assessment tasks identified from 1,227 assessment tasks in CET and TEM examinations administered between 2010 and 2024. A three-stage coding procedure was used to identify value-related texts and classify them into four higher-order dimensions-openness to change, self-transcendence, conservation, and self-enhancement. Questionnaire data from 1,066 students and interviews with seven participants were analyzed to explore stakeholder perceptions. Results indicate that all four value dimensions are present but unevenly distributed, with openness to change and self-transcendence more prominently represented. Students reported high awareness and general acceptance of value-oriented content. Notably, students' reported interest did not simply correspond to the frequency with which value-related themes appeared in the assessment corpus. Interview findings further suggested that students generally interpreted value-related themes as part of the thematic context of assessment texts rather than as explicit moral messages. By using Schwartz's value framework as a descriptive taxonomy alongside a critical perspective on assessment discourse, this study advances understanding of how value-related content is represented in institutional assessment contexts and interpreted by student stakeholders. CI - Copyright (c) 2026 Luo, Dai and Meng. FAU - Luo, Juan AU - Luo J AD - School of Foreign Studies, Changsha University of Science and Technology, Changsha, China. FAU - Dai, Ningze AU - Dai N AD - School of Foreign Studies, Changsha University of Science and Technology, Changsha, China. FAU - Meng, Chengye AU - Meng C AD - School of Foreign Studies, Changsha University of Science and Technology, Changsha, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Psychol JT - Frontiers in psychology JID - 101550902 PMC - PMC13713726 OTO - NOTNLM OT - basic human values theory OT - cognitive activation OT - large-scale language assessment OT - motivational salience OT - stakeholder perceptions COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:33 PHST- 2026/03/01 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:33 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpsyg.2026.1820640 [doi] PST - epublish SO - Front Psychol. 2026 Oct 14;17:1820640. doi: 10.3389/fpsyg.2026.1820640. eCollection 2026. PMID- 42953078 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2297-055X (Print) IS - 2297-055X (Electronic) IS - 2297-055X (Linking) VI - 13 DP - 2026 TI - Assessment of myocardial perfusion using dual-source computed tomography myocardial perfusion imaging in a goat model of reperfusion injury induced by distal microembolization. PG - 1809725 LID - 10.3389/fcvm.2026.1809725 [doi] LID - 1809725 AB - BACKGROUND: Myocardial ischemia-reperfusion injury (MIRI) contributes to adverse outcomes after acute myocardial infarction. Microvascular obstruction (MVO), a key component of MIRI, impairs myocardial perfusion despite successful epicardial revascularization. This study aimed to establish a reproducible large-animal model of embolization-induced MVO and evaluate myocardial perfusion heterogeneity using dual-source CT myocardial perfusion imaging (DSCT-MPI). METHODS: Eight male Saanen goats underwent 30 min of left circumflex artery occlusion followed by distal microcatheter injection of 20-mum polystyrene microspheres to induce reperfusion-associated MVO. DSCT-MPI was performed immediately after model establishment, with myocardial perfusion assessed by iodine distribution. Pathology was evaluated using triphenyltetrazolium chloride and hematoxylin-eosin staining, and Bcl-2, Bax, and caspase-3 were quantified. Receiver operating characteristic (ROC) analysis was used to determine the iodine concentration threshold for infarcted myocardium. RESULTS: Seven of eight goats survived and underwent DSCT-MPI. Of 119 myocardial segments, 39 were infarcted, 14 were non-infarcted hypoperfused segments consistent with distal MVO, and 66 were normal. Binary ROC and diagnostic-performance analyses included the 39 infarcted and 66 normal segments, excluding the 14 MVO-consistent segments. At an iodine concentration cutoff of 1,582 mug/cm(3), DSCT-MPI achieved 89.7% sensitivity, 90.9% specificity, and an AUC of 0.848 (95% CI, 0.736-0.959), with substantial histopathological agreement (kappa = 0.798). Infarcted myocardium showed reduced Bcl-2 and increased Bax and caspase-3 expression. The 14 hypoperfused segments without TTC-confirmed necrosis were classified as MVO-consistent rather than CMD. CONCLUSIONS: A minimally invasive, reproducible goat model of embolization-induced myocardial reperfusion injury was established. DSCT-MPI identified infarcted myocardium and non-infarcted hypoperfused regions consistent with distal MVO, demonstrating post-reperfusion perfusion heterogeneity without supporting CMD. Because no direct CMR comparison was performed, these findings should be considered exploratory pathology-based validation. CI - (c) 2026 Jin, Duan, Chen, Lu, Liu and Li. FAU - Jin, Hui AU - Jin H AD - Department of Cardiology, Baotou Central Hospital, Baotou, Inner Mongolia, China. FAU - Duan, Chaowei AU - Duan C AD - Department of Cardiology, Baotou Central Hospital, Baotou, Inner Mongolia, China. FAU - Chen, Xiaolin AU - Chen X AD - Department of Cardiology, Baotou Central Hospital, Baotou, Inner Mongolia, China. FAU - Lu, Yaojun AU - Lu Y AD - Department of Cardiology, Baotou Central Hospital, Baotou, Inner Mongolia, China. FAU - Liu, Xiaolin AU - Liu X AD - Department of Radiology, Baotou Central Hospital, Baotou, Inner Mongolia, China. FAU - Li, Meng AU - Li M AD - Department of Cardiology, Baotou Central Hospital, Baotou, Inner Mongolia, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Cardiovasc Med JT - Frontiers in cardiovascular medicine JID - 101653388 PMC - PMC13713717 OTO - NOTNLM OT - DSCT-MPI OT - goat model OT - microspheres OT - microvascular obstruction OT - myocardial ischemia-reperfusion injury OT - myocardial perfusion imaging COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:33 PHST- 2026/02/12 00:00 [received] PHST- 2026/07/03 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:33 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fcvm.2026.1809725 [doi] PST - epublish SO - Front Cardiovasc Med. 2026 Oct 14;13:1809725. doi: 10.3389/fcvm.2026.1809725. eCollection 2026. PMID- 42953075 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Autophagy-lysosome pathway dysfunction in MASLD progression: molecular mechanisms and therapeutic targets. PG - 1961366 LID - 10.3389/fmed.2026.1961366 [doi] LID - 1961366 AB - Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most prevalent chronic liver disease worldwide, yet no standardized pharmacotherapy has been approved for clinical use. The autophagy-lysosome pathway (ALP) serves as an essential intracellular degradation and recycling machinery that sustains hepatic metabolic homeostasis via modulation of lipid turnover, mitochondrial quality surveillance, and hepatocellular detoxification. Accumulating evidence demonstrates that ALP impairment acts as a core pathogenic factor across all stages of MASLD progression. This review systematically elaborates the molecular framework of the ALP and its multifaceted physiological functions in the liver, with special attention to the central pathogenic mechanisms underlying ALP impairment in MASLD: lysosomal dysfunction, defective autophagosome-lysosome fusion, dysregulation of key transcriptional and epigenetic regulators, and impaired subtypes of selective autophagy. ALP activity displays stage-specific characteristics across MASLD progression, featuring compensatory activation in simple hepatic steatosis but functional suppression in metabolic dysfunction-associated steatohepatitis (MASH). Hepatocytes and hepatic stellate cells exert opposing autophagic activity during liver fibrogenesis. We further summarize existing therapeutic strategies targeting the ALP, which fall into three categories: pharmacological agents (ALP agonists, lysosomal modulators, natural bioactive compounds, and nano-drug delivery systems), non-pharmacological interventions (dietary adjustment, physical exercise, and lifestyle modification), and gut-liver axis regulatory approaches. Finally, we address the primary translational bottlenecks and prospective avenues for converting these mechanistic findings into viable clinical therapies. CI - (c) 2026 Cao, Cheng, Yang, Lu, Li, Huang, Ba, Luo and Zhao. FAU - Cao, Shuaibing AU - Cao S AD - The Second Clinical Medical College, Henan University of Chinese Medicine, Zhengzhou, China. FAU - Cheng, Xinyue AU - Cheng X AD - The Second Clinical Medical College, Henan University of Chinese Medicine, Zhengzhou, China. FAU - Yang, Yi AU - Yang Y AD - Medical College, Henan University of Chinese Medicine, Zhengzhou, China. FAU - Lu, Baoping AU - Lu B AD - The Second Clinical Medical College, Henan University of Chinese Medicine, Zhengzhou, China. AD - Henan Medical College, Zhengzhou, China. FAU - Li, Shan AU - Li S AD - Medical College, Henan University of Chinese Medicine, Zhengzhou, China. FAU - Huang, Chuntian AU - Huang C AD - Medical College, Henan University of Chinese Medicine, Zhengzhou, China. FAU - Ba, Mingyu AU - Ba M AD - The First Affiliated Hospital of Henan University of TCM, Zhengzhou, China. FAU - Luo, Lei AU - Luo L AD - The Second Affiliated Hospital of Henan University of TCM, Zhengzhou, China. FAU - Zhao, Changpu AU - Zhao C AD - The Second Clinical Medical College, Henan University of Chinese Medicine, Zhengzhou, China. AD - The Second Affiliated Hospital of Henan University of TCM, Zhengzhou, China. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13714512 OTO - NOTNLM OT - autophagy-lysosome pathway OT - lysosomal dysfunction OT - metabolic dysfunction-associated steatotic liver disease OT - metabolism OT - targeted therapy COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:33 PHST- 2026/08/07 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:33 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmed.2026.1961366 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 14;13:1961366. doi: 10.3389/fmed.2026.1961366. eCollection 2026. PMID- 42953073 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2632-1297 (Electronic) IS - 2632-1297 (Linking) VI - 8 IP - 5 DP - 2026 TI - Multimodal eye-tracking reveals impaired inhibitory control and reduced preparatory pupil dilation in essential tremor. PG - fcag389 LID - 10.1093/braincomms/fcag389 [doi] LID - fcag389 AB - Essential tremor (ET) is increasingly recognized as a disorder involving cerebello-thalamo-cortical and autonomic processes beyond tremor itself. Oculomotor, pupillary and blink signals provide complementary physiological readouts of these distributed systems, yet they have largely been studied in isolation. Here, we used video-based eye tracking during an interleaved pro- and anti-saccade task to characterize oculomotor performance, pupil dynamics, blink behaviour and trial-by-trial cross-domain coupling in 45 ET patients and 46 controls. ET showed impaired inhibitory oculomotor control, reflected by increased direction errors, prolonged anti-saccade reaction times, extended voluntary override time and reduced generation of express saccades. Preparatory pupil dilation was attenuated and constriction timing delayed despite a preserved tonic baseline. In contrast, blink dynamics were largely preserved. Trial-by-trial pupil-saccade coupling was also largely preserved, indicating maintained cross-domain coordination despite reduced preparatory autonomic modulation. Exploratory machine-learning analyses suggested that combined pupil-saccade features may capture aspects of clinical heterogeneity, particularly early onset status, although these findings did not survive permutation testing. Together, these results indicate that ET is associated with selective impairment of inhibitory oculomotor control and preparatory autonomic responses, with preserved cross-domain coordination. Multimodal eye tracking may therefore provide a scalable, non-invasive framework for characterizing the heterogeneous physiological expression of ET. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Guarantors of Brain. FAU - Hong, Chien-Tai AU - Hong CT AUID- ORCID: 0000-0002-7448-1041 AD - Department of Neurology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan. AD - Department of Neurology, Shuang Ho Hospital, Taipei Medical University, Taipei 235, Taiwan. FAU - Chen, Jui-Tai AU - Chen JT AD - Department of Anesthesiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan. AD - Department of Anesthesiology, Shuang Ho Hospital, Taipei Medical University, Taipei 235, Taiwan. FAU - Yong, Audrey AU - Yong A AUID- ORCID: 0009-0000-3228-5766 AD - Eye-Tracking Laboratory, Shuang Ho Hospital, Taipei Medical University, Taipei 235, Taiwan. AD - College of Medicine, Chang Gung University, Taoyuan 333, Taiwan. FAU - Chen, Ta-Liang AU - Chen TL AUID- ORCID: 0000-0002-1156-2389 AD - Department of Anesthesiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan. AD - Health Policy Research Center, Taipei Medical University Hospital, Taipei 110, Taiwan. AD - Department of Anesthesiology, Wang Fang Hospital, Taipei Medical University, Taipei 116, Taiwan. FAU - Xu, Kevin S AU - Xu KS AD - Department of Computer and Data Sciences, Case Western Reserve University, Cleveland, OH 44106, USA. FAU - Wang, Chin-An AU - Wang CA AUID- ORCID: 0000-0002-6203-7701 AD - Department of Anesthesiology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan. AD - Department of Anesthesiology, Shuang Ho Hospital, Taipei Medical University, Taipei 235, Taiwan. AD - Eye-Tracking Laboratory, Shuang Ho Hospital, Taipei Medical University, Taipei 235, Taiwan. AD - Department of Education and Humanities in Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan. AD - Ph.D. Program in Medical Neuroscience, College of Medical Science and Technology, Taipei Medical University, Taipei 235, Taiwan. LA - eng PT - Journal Article DEP - 20261015 PL - England TA - Brain Commun JT - Brain communications JID - 101755125 PMC - PMC13714541 OTO - NOTNLM OT - autonomic function OT - essential tremor OT - inhibitory control OT - oculomotor control OT - pupillometry COIS- The authors report no competing interests. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/15 CRDT- 2026/10/29 05:33 PHST- 2026/04/09 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:33 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - fcag389 [pii] AID - 10.1093/braincomms/fcag389 [doi] PST - epublish SO - Brain Commun. 2026 Oct 15;8(5):fcag389. doi: 10.1093/braincomms/fcag389. eCollection 2026. PMID- 42953072 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2673-8740 (Electronic) IS - 2673-8740 (Linking) VI - 6 DP - 2026 TI - Topology-preserving coronary vessel segmentation in X-ray angiography using ResNet50-MASAG-UNet. PG - 1949450 LID - 10.3389/fradi.2026.1949450 [doi] LID - 1949450 AB - Coronary artery disease occurs when the accumulation of fatty atheroma deposits narrows the coronary blood vessels, thereby reducing blood flow to the myocardium. To identify these narrowings, cardiologists use X-ray coronary angiography (XCA). However, these angiographic images are often blurry and noisy, making vessels difficult to view clearly. To enhance visibility, we proposed an image improvement technique that combines three filtering approaches: Bilateral filtering, unsharp masking, and Contrast Limited Adaptive Histogram Equalization (CLAHE), to remove noise, sharpen vessel edges, and improve brightness balance, producing clearer vessel images to support subsequent analysis. Our proposed ResNet50-MASAG-UNet integrates a Multi-Scale Adaptive Spatial Attention Gate (MASAG) at each skip connection of a ResNet50-UNet, enabling the decoder to attend selectively to coronary vessel regions while suppressing non-vascular activations from catheters and bones. A topology-aware loss is used to preserve vessel connectivity by maintaining centreline continuity. Together, these elements enable more accurate and complete coronary vessel segmentation. The proposed model achieves improved segmentation performance on XCA images, attaining a Dice coefficient of 88.3%, a Jaccard Index of 79.1%, a precision of 86.4%, and a recall of 90.5%. It outperforms the standard UNet baseline and provides clearer vessel segmentation, demonstrating improved potential for coronary vessel analysis. CI - (c) 2026 Sunilkumar and Kumaresan. FAU - Sunilkumar, G AU - Sunilkumar G AD - School of Computer Science Engineering and Information Systems (SCORE), Vellore Institute of Technology, Vellore, India. FAU - Kumaresan, P AU - Kumaresan P AD - Centre For Artificial Intelligence Research (C-FAIR), Vellore Institute of Technology, Vellore, India. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Radiol JT - Frontiers in radiology JID - 9918367586306676 PMC - PMC13713719 OTO - NOTNLM OT - ResNet50-MASAG-UNet OT - X-ray coronary angiography OT - attention gates OT - coronary artery disease OT - vessel segmentation OT - vessel visualization COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:33 PHST- 2026/07/27 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:33 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fradi.2026.1949450 [doi] PST - epublish SO - Front Radiol. 2026 Oct 14;6:1949450. doi: 10.3389/fradi.2026.1949450. eCollection 2026. PMID- 42953067 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2398-8835 (Electronic) IS - 2398-8835 (Linking) VI - 9 IP - 11 DP - 2026 Nov TI - Investigating the Relationship Between Self-Compassion and Marital Satisfaction in Pregnant Women With Depression: A Cross-Sectional Study. PG - e73439 LID - 10.1002/hsr2.73439 [doi] LID - e73439 AB - BACKGROUND AND AIMS: Antenatal depression is a prevalent mental health concern that can adversely affect marital relationships. Self-compassion has emerged as a potential protective factor in improving psychological health and interpersonal relationships. This cross-sectional study aimed to investigate the relationship between self-compassion and marital satisfaction in pregnant women with depression. METHODS: In this cross-sectional analytical study, 240 pregnant women with depression were selected through convenience sampling from healthcare centers in Kermanshah, Iran. Depression was assessed using the Edinburgh Postnatal Depression Scale (EPDS) with a cutoff score > 12, and all participants met DSM-5 criteria for depressive symptoms. Participants completed the Self-Compassion Scale-Long Form, Hudson's Marital Satisfaction Inventory, and a demographic questionnaire. The cross-sectional design and convenience sampling limit the generalizability of findings. RESULTS: The mean age was 27.68 years (SD = 4.63). Logistic regression revealed that a one-unit increase in total self-compassion score was associated with a 10% increase in the odds of being in the satisfied group (OR = 1.10; 95% CI: 1.07-1.12; p < 0.001). "Common human experience" showed the strongest association (OR = 1.64; 95% CI: 1.43-1.87; p < 0.001). Positive components-self-kindness (r = 0.552), common humanity (r = 0.561), and well-being (r = 0.519)-had significant positive correlations with marital satisfaction, while negative components-self-judgment (r = -0.462), isolation (r = -0.506), and over-identification (r = -0.446)-showed significant negative correlations (all p < 0.001). CONCLUSION: Self-compassion, particularly its positive components, is significantly associated with higher marital satisfaction in depressed pregnant women. Self-compassion-based interventions may be a promising strategy to improve marital relationship quality and maternal mental health during pregnancy. CI - (c) 2026 The Author(s). Health Science Reports published by Wiley Periodicals LLC. FAU - Ashabi, Bita AU - Ashabi B AUID- ORCID: 0009-0003-8666-2442 AD - Research Committee of the School of Nursing and Midwifery Kermanshah University of Medical Sciences Kermanshah Iran. AD - School of Nursing and Midwifery Hamadan University of Medical Sciences Hamadan Iran. FAU - Haseli, Arezoo AU - Haseli A AUID- ORCID: 0000-0003-1487-633X AD - Department of Midwifery Education, School of Nursing and Midwifery, Family Health and Population Growth Research Center, Health Policy and Promotion Research Institute Kermanshah University of Medical Sciences Kermanshah Iran. FAU - Rezaeian, Shahab AU - Rezaeian S AUID- ORCID: 0000-0002-5094-5315 AD - Department of Epidemiology School of Health, Infectious Diseases Research Center, Health Policy and Promotion Institute, Imam Reza Hospital, Kermanshah University of Medical Sciences Kermanshah Iran. FAU - Mahmoodi, Ehsan AU - Mahmoodi E AD - Research Committee of the School of Nursing and Midwifery Kermanshah University of Medical Sciences Kermanshah Iran. FAU - Kamravamanesh, Mastaneh AU - Kamravamanesh M AUID- ORCID: 0000-0002-2283-4554 AD - Department of Reproductive Health, School of Nursing and Midwifery, Clinical Research Development Center, Motazedi Hospital Kermanshah University of Medical Sciences Kermanshah Iran. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Health Sci Rep JT - Health science reports JID - 101728855 PMC - PMC13714565 OTO - NOTNLM OT - depression OT - marital satisfaction OT - pregnancy OT - self-compassion COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/28 CRDT- 2026/10/29 05:33 PHST- 2025/08/14 00:00 [received] PHST- 2026/07/02 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:33 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - HSR273439 [pii] AID - 10.1002/hsr2.73439 [doi] PST - epublish SO - Health Sci Rep. 2026 Oct 28;9(11):e73439. doi: 10.1002/hsr2.73439. eCollection 2026 Nov. PMID- 42953053 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2634-3916 (Electronic) IS - 2634-3916 (Linking) VI - 7 IP - 9 DP - 2026 Nov TI - Deep learning analysis of sinus-rhythm echocardiography for the identification of patients with atrial fibrillation. PG - ztag168 LID - 10.1093/ehjdh/ztag168 [doi] LID - ztag168 AB - AIMS: Paroxysmal atrial fibrillation (AF) is underdiagnosed due to its intermittent and asymptomatic nature. We evaluated whether a deep neural network (DNN) applied to transthoracic echocardiograms (TTEs) acquired in sinus rhythm can discriminate between patients with and without documented AF and identify individuals at increased risk for subsequent AF documentation and adverse outcomes. METHODS AND RESULTS: We retrospectively analysed 85 686 TTE studies (2009-21). A DNN was applied to apical four-chamber and parasternal long-axis videos without Doppler. AF ground truth was based on International Classification of Diseases (ICD) codes, electrocardiogram, Holter, or prior echocardiographic documentation of AF. The dataset was split into training (60%), validation (20%), and an independent test set (20%). In the test cohort, we assessed discrimination performance. Among patients without prior AF, we evaluated associations between model classification and subsequent AF documentation and a composite outcome of all-cause mortality, heart failure hospitalization, or stroke. The test set included 17 121 patients (median age 66 years [interquartile range, IQR 54-75]; 57.6% male), of whom 3449 (20.1%) had prior AF. The model achieved an area under the receiver operating characteristic curve of 0.85, with sensitivity 0.80 and specificity 0.72. At the selected threshold, 6581 patients (38.4%) were classified as model-positive, including 3832 (58.2%) without prior AF. Among patients without prior AF, model-positive classification was associated with subsequent AF documentation [adjusted hazard ratio (HR) 1.64; 95% confidence interval (CI) 1.33-2.04] and with the composite outcome (adjusted HR 1.24; 95% CI 1.13-1.35). CONCLUSION: Deep learning applied to sinus-rhythm TTE demonstrates discrimination for AF and identifies an imaging-derived signal associated with subsequent AF documentation and adverse outcomes. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the European Society of Cardiology. FAU - Cohen, Ido AU - Cohen I AUID- ORCID: 0000-0003-4528-8194 AD - Leviev Cardiovascular Institute, Sheba Medical Center, Ramat Gan, Israel. AD - Faculty of Medical and Health Sciences, Tel Aviv University, Tel Aviv, Israel. FAU - Fiman, Michael AU - Fiman M AUID- ORCID: 0009-0008-3075-1506 AD - Aisap.ai, Ramat Gan, Israel. FAU - Loutati, Ranel AU - Loutati R AUID- ORCID: 0009-0008-9012-9540 AD - Leviev Cardiovascular Institute, Sheba Medical Center, Ramat Gan, Israel. AD - Faculty of Medical and Health Sciences, Tel Aviv University, Tel Aviv, Israel. FAU - Schwammenthal, Ehud AU - Schwammenthal E AUID- ORCID: 0009-0001-7065-8647 AD - Leviev Cardiovascular Institute, Sheba Medical Center, Ramat Gan, Israel. AD - Faculty of Medical and Health Sciences, Tel Aviv University, Tel Aviv, Israel. AD - Aisap.ai, Ramat Gan, Israel. FAU - Faierstein, Kobi AU - Faierstein K AD - Leviev Cardiovascular Institute, Sheba Medical Center, Ramat Gan, Israel. AD - Faculty of Medical and Health Sciences, Tel Aviv University, Tel Aviv, Israel. FAU - Fisher, Lior AU - Fisher L AD - Leviev Cardiovascular Institute, Sheba Medical Center, Ramat Gan, Israel. AD - Faculty of Medical and Health Sciences, Tel Aviv University, Tel Aviv, Israel. FAU - Maor, Elad AU - Maor E AUID- ORCID: 0000-0002-5087-0464 AD - Leviev Cardiovascular Institute, Sheba Medical Center, Ramat Gan, Israel. AD - Faculty of Medical and Health Sciences, Tel Aviv University, Tel Aviv, Israel. AD - Aisap.ai, Ramat Gan, Israel. LA - eng PT - Journal Article DEP - 20261021 PL - England TA - Eur Heart J Digit Health JT - European heart journal. Digital health JID - 101778323 PMC - PMC13714549 OTO - NOTNLM OT - Arrhythmia detection OT - Artificial intelligence OT - Atrial fibrillation OT - Deep learning OT - Echocardiography OT - Risk stratification COIS- Conflict of interest: Drs. Schwammenthal and Maor are co-founders of Aisap.ai. Mr Fiman is employed by Aisap.ai. All other authors declare no competing interests. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/21 CRDT- 2026/10/29 05:32 PHST- 2026/04/20 00:00 [received] PHST- 2026/06/30 00:00 [revised] PHST- 2026/08/11 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ztag168 [pii] AID - 10.1093/ehjdh/ztag168 [doi] PST - epublish SO - Eur Heart J Digit Health. 2026 Oct 21;7(9):ztag168. doi: 10.1093/ehjdh/ztag168. eCollection 2026 Nov. PMID- 42953052 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-2565 (Electronic) IS - 2296-2565 (Linking) VI - 14 DP - 2026 TI - Body mass index and health-related lifestyle behaviors among university students in Saudi Arabia: a single center cross-sectional study. PG - 1900451 LID - 10.3389/fpubh.2026.1900451 [doi] LID - 1900451 AB - BACKGROUND: Overweight and obesity are important public-health concerns among young adults. University students experience changes in dietary, physical-activity, sleep, and other health behaviors, but evidence from Saudi university populations remains heterogeneous. OBJECTIVE: To explore associations between BMI and selected dietary, physical-activity, sleep, and sociodemographic characteristics among university students. METHODS: This cross-sectional study analyzed electronic questionnaire responses collected from January to April 2025 on a sample of 196 students. Weight and height were self-reported and BMI was recalculated as weight (kg)/height(2) (m(2)). BMI was categorized as underweight (<18.5), normal (18.5 to <24.9), overweight (25 to <30), or obesity (>/=30 kg/m(2)). Associations were examined using contingency-table analyses and limited exploratory multivariable linear and logistic regression models. RESULTS: Among 196 analyzed students, 17 (8.7%) were underweight, 106 (54.1%) had normal BMI, 38 (19.4%) were overweight, and 35 (17.9%) had obesity; thus, 73 (37.2%) had overweight or obesity. Associations of BMI category with lifestyle were not statistically significant. Electronic-device use before bed showed a sparse-cell association (chi (2)=23.438, df = 9, Monte Carlo p = 0.010) that should be considered exploratory. In adjusted linear regression, male sex (beta = 5.26 kg/m(2), 95% CI 2.36-8.16, p < 0.001), and students aged >23 years had higher BMI (beta = 2.86, 95% CI 0.44-5.29, p = 0.021). In adjusted logistic regression, male sex (OR = 2.71, 95% CI 1.19-6.19, p = 0.018) and age >23 years (OR = 3.15, 95% CI 1.17-8.43, p = 0.023) were associated with overweight/obesity. CONCLUSION: Overweight and obesity were common. Gender and age showed associations with BMI status, whereas evidence for independent associations of the selected lifestyle measures was limited and imprecise. The cross-sectional design and self-reported measurements preclude causal inference. CI - Copyright (c) 2026 Alafif, Tharkar, Alharbi, Althobaiti, Alamry, Al-harbi and Alfawaz. FAU - Alafif, Nora AU - Alafif N AD - Department of Community Health Science, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia. FAU - Tharkar, Shabana AU - Tharkar S AD - Department of Community Health Science, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia. FAU - Alharbi, Rinas AU - Alharbi R AD - Department of Community Health Science, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia. FAU - Althobaiti, Hadeel AU - Althobaiti H AD - Department of Community Health Science, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia. FAU - Alamry, Rawa AU - Alamry R AD - Department of Community Health Science, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia. FAU - Al-Harbi, Reema AU - Al-Harbi R AD - Department of Community Health Science, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia. FAU - Alfawaz, Waad AU - Alfawaz W AD - Department of Community Health Science, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Public Health JT - Frontiers in public health JID - 101616579 SB - IM MH - Humans MH - Cross-Sectional Studies MH - Saudi Arabia/epidemiology MH - Male MH - Female MH - *Body Mass Index MH - *Students/statistics & numerical data/psychology MH - Universities MH - Young Adult MH - *Life Style MH - *Health Behavior MH - Adult MH - Exercise MH - Obesity/epidemiology MH - Adolescent MH - Overweight/epidemiology MH - Surveys and Questionnaires PMC - PMC13713735 OTO - NOTNLM OT - Saudi Arabia OT - body mass index OT - dietary behavior OT - physical activity OT - university students COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:32 PHST- 2026/06/04 00:00 [received] PHST- 2026/09/07 00:00 [revised] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:32 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpubh.2026.1900451 [doi] PST - epublish SO - Front Public Health. 2026 Oct 14;14:1900451. doi: 10.3389/fpubh.2026.1900451. eCollection 2026. PMID- 42953051 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 17 DP - 2026 TI - Gut microbiota and intestinal permeability in rheumatoid arthritis: therapeutic implications and future directions. PG - 1936364 LID - 10.3389/fimmu.2026.1936364 [doi] LID - 1936364 AB - Interactions between the intestinal microbiota, epithelial barrier and mucosal immune system have prompted investigation of gut-directed interventions in rheumatoid arthritis (RA). This review examines whether modifying these interactions can improve clinical outcomes and assesses the strength of the available evidence in RA. In experimental arthritis, interventions that preserve epithelial integrity or alter microbial metabolism can reduce disease severity, providing a biological basis for further study. However, their relevance to human RA remains uncertain, since effects observed in animal models do not establish clinical efficacy, and changes in intestinal measurements alone do not demonstrate improvement in joint disease. Future controlled trials should assess disease activity, and physical function alongside microbial composition and function, intestinal measurements and immune responses. Repeated sampling may help establish whether intestinal changes precede or accompany clinical improvement, while recording medication exposure and dietary intake is needed to interpret these relationships. Candidate biomarkers will require prospective validation before they can be used to select patients for treatment. These studies should determine whether gut-directed interventions provide additional clinical benefit, in whom, and at what risk. CI - Copyright (c) 2026 Bilski, Schramm-Luc, Szczepanik, Pierzchalski and Luc. FAU - Bilski, Jan AU - Bilski J AD - Department of Biomechanics and Kinesiology, Chair of Biomedical Sciences, Institute of Physiotherapy, Faculty of Health Sciences, Jagiellonian University Medical College, Krakow, Poland. FAU - Schramm-Luc, Agata I AU - Schramm-Luc AI AD - Department of Internal and Agricultural Medicine, Faculty of Medicine, Jagiellonian University Medical College, Krakow, Poland. FAU - Szczepanik, Marian AU - Szczepanik M AD - Chair of Biomedical Sciences, Institute of Physiotherapy, Faculty of Health Sciences, Jagiellonian University Medical College, Krakow, Poland. FAU - Pierzchalski, Piotr AU - Pierzchalski P AD - Department of Medical Physiology, Chair of Biomedical Sciences, Institute of Physiotherapy, Faculty of Health Sciences, Jagiellonian University Medical College, Krakow, Poland. FAU - Luc, Kevin AU - Luc K AD - Department of Internal and Agricultural Medicine, Faculty of Medicine, Jagiellonian University Medical College, Krakow, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 SB - IM MH - Humans MH - *Arthritis, Rheumatoid/microbiology/therapy/immunology/metabolism MH - Animals MH - *Gastrointestinal Microbiome/immunology MH - Intestinal Barrier Function MH - *Intestinal Mucosa/metabolism/microbiology/immunology MH - Permeability MH - Immunity, Mucosal PMC - PMC13714585 OTO - NOTNLM OT - gut-joint axis OT - intestinal microbiota OT - intestinal permeability OT - microbial metabolites OT - mucosal immunity OT - physical activity OT - rheumatoid arthritis OT - short-chain fatty acids COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:32 PHST- 2026/07/13 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:32 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fimmu.2026.1936364 [doi] PST - epublish SO - Front Immunol. 2026 Oct 14;17:1936364. doi: 10.3389/fimmu.2026.1936364. eCollection 2026. PMID- 42953043 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2054-5703 (Print) IS - 2054-5703 (Electronic) IS - 2054-5703 (Linking) VI - 13 IP - 10 DP - 2026 Oct 7 TI - Mechanistic ODE modelling of ovarian tumour microenvironment dynamics under hesperidin exposure. PG - 260368 LID - 10.1098/rsos.260368 [doi] LID - 260368 AB - Ovarian cancer progression is driven by complex interactions among tumour, immune, stromal, metabolic and angiogenic processes. Although hesperidin exhibits anti-cancer and immunomodulatory activities, its integrated effects on the tumour microenvironment (TME) remain insufficiently understood. This study presents a mechanistic ODE-based framework integrating tumour growth, immune regulation, macrophage polarization, cancer-associated fibroblast (CAF)-mediated extracellular matrix (ECM) remodelling, hypoxia-inducible factor (HIF) - 1alpha/ vascular endothelial growth factor (VEGF)-dependent angiogenesis, glucose-lactate metabolism and redox signalling within a hypothetical ovarian TME centred on PA-1 cells. Simulations across multiple hesperidin concentrations predicted dose-dependent suppression of tumour growth, reduced HIF - 1alpha accumulation, angiogenesis, ECM stiffening and lactate production, accompanied by enhanced anti-tumour immune activity. Local one-at-a-time (OAT) and global Sobol' sensitivity analyses identified tumour growth kinetics, CAF-ECM interactions, metabolic regulation and selected hesperidin-response parameters as principal determinants of system behaviour. Although not calibrated for quantitative prediction, the proposed framework provides mechanistic insight into how hesperidin-mediated perturbations propagate through interconnected tumour-immune-stromal networks. The model should be regarded as an exploratory, hypothesis-generating platform supporting future experimental validation of hesperidin-mediated mechanisms in ovarian cancer. CI - (c) 2026 The Authors. FAU - Dzik, Radoslaw AU - Dzik R AUID- ORCID: 0000-0002-6289-7234 AD - Department of Clinical Engineering, Academy of Silesia, Katowice, Poland. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - R Soc Open Sci JT - Royal Society open science JID - 101647528 PMC - PMC13714397 OTO - NOTNLM OT - ODE OT - PA-1 OT - hesperidin OT - mechanistic model OT - ovarian cancer COIS- The author declares no competing interests. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/28 CRDT- 2026/10/29 05:31 PHST- 2026/02/20 00:00 [received] PHST- 2026/06/09 00:00 [revised] PHST- 2026/08/25 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:31 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - rsos260368 [pii] AID - 10.1098/rsos.260368 [doi] PST - epublish SO - R Soc Open Sci. 2026 Oct 28;13(10):260368. doi: 10.1098/rsos.260368. eCollection 2026 Oct 7. PMID- 42953039 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1663-9812 (Print) IS - 1663-9812 (Electronic) IS - 1663-9812 (Linking) VI - 17 DP - 2026 TI - Targeting pain where it CouNts: the role of neurotensin signaling in pain and analgesia. PG - 1907900 LID - 10.3389/fphar.2026.1907900 [doi] LID - 1907900 AB - Chronic pain affects more than one-fifth of adults in the United States and represents a major public health burden due to its impact on quality of life, productivity, and healthcare costs. Current pharmacological treatments remain limited in efficacy and are often associated with significant adverse effects, highlighting the need for safer and more effective therapeutic strategies. Neurotensin (Nts), a neuropeptide widely expressed in the central and peripheral nervous systems, has emerged as an important modulator of nociceptive processing. Nts signals through the G protein-coupled receptors neurotensin receptor 1 (NTSR1) and neurotensin receptor 2 (NTSR2), which are expressed in key neuroanatomical components of pain pathways. In this review, we summarize current knowledge of the neuronal and molecular mechanisms underlying pain processing and discuss how Nts signaling modulates nociceptive transmission at peripheral, spinal, and supraspinal levels. In addition to regulating nociceptor excitability and spinal excitatory neurotransmission, Nts critically regulates neural circuits involved in descending pain modulation, including the periaqueductal gray and rostral ventromedial medulla, and contributes to pain processing within limbic and hypothalamic networks that integrate sensory and affective components of pain. Finally, we discuss recent advances in pharmacological modulation of Nts receptors, including biased allosteric modulators and receptor-selective agonists that show promising analgesic efficacy in preclinical models. Together, these findings position the neurotensin system as a promising target for the development of novel non-opioid therapies for pain. CI - Copyright (c) 2026 Chapman, Black, Leinninger and Laumet. FAU - Chapman, Alex D AU - Chapman AD AD - Neuroscience Program, Michigan State University, East Lansing, MI, United States. AD - Department of Physiology, Michigan State University, East Lansing, MI, United States. FAU - Black, Katherine L AU - Black KL AD - Neuroscience Program, Michigan State University, East Lansing, MI, United States. AD - Department of Physiology, Michigan State University, East Lansing, MI, United States. FAU - Leinninger, Gina M AU - Leinninger GM AD - Neuroscience Program, Michigan State University, East Lansing, MI, United States. AD - Department of Physiology, Michigan State University, East Lansing, MI, United States. FAU - Laumet, Geoffroy AU - Laumet G AD - Neuroscience Program, Michigan State University, East Lansing, MI, United States. AD - Department of Physiology, Michigan State University, East Lansing, MI, United States. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Pharmacol JT - Frontiers in pharmacology JID - 101548923 PMC - PMC13714406 OTO - NOTNLM OT - GPCR OT - analgesia OT - neuropharmacology OT - neurotensin OT - nociception OT - pain COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:31 PHST- 2026/06/12 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:31 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1907900 [pii] AID - 10.3389/fphar.2026.1907900 [doi] PST - epublish SO - Front Pharmacol. 2026 Oct 14;17:1907900. doi: 10.3389/fphar.2026.1907900. eCollection 2026. PMID- 42953037 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2048-7177 (Print) IS - 2048-7177 (Electronic) IS - 2048-7177 (Linking) VI - 14 IP - 11 DP - 2026 Nov TI - Comparative Analysis of Traditional and Modern Extraction Techniques for Phenolic Compounds From Leontopodium and Optimization of Extraction Process Using Response Surface Methodology. PG - e72523 LID - 10.1002/fsn3.72523 [doi] LID - e72523 AB - Phenolic compounds from Leontopodium leontopodioides (LLB) are natural bioactive constituents with potential antioxidant activity. This study compared reflux extraction (RE), microwave-assisted extraction (MAE), and ultrasound-assisted extraction (UAE) for recovering total flavonoids (TFC) and total phenolic content (TPC) from LLB. Based on the comparative results, UAE was further optimized using response surface methodology. The effects of habitat on TFC and TPC contents and the antioxidant activity of LLB extracts were also evaluated. Under the tested operating conditions, UAE with 70% ethanol showed the best overall extraction performance and was therefore selected for optimization. The optimized UAE conditions for TFC were an extraction time of 61 min, a solid-to-liquid ratio of 1:27 (g/mL), an ultrasound power of 610 W, and an extraction temperature of 58 degrees C, yielding 40.58 +/- 0.94 mg/g DW. For TPC, the optimum conditions were 40 min, 1:24 (g/mL), 430 W, and 57 degrees C, respectively, yielding 26.38 +/- 0.57 mg/g DW. LLB collected from desert grassland exhibited the highest TFC content and the strongest DPPH-, ABTS-, and hydroxyl-radical-scavenging activities, as well as the greatest reducing power. In contrast, TPC did not differ significantly among habitats (p > 0.05). These findings provide a basis for optimizing the extraction of phenolic constituents from LLB and support its future processing and utilization as a source of bioactive ingredients. CI - (c) 2026 The Author(s). Food Science & Nutrition published by Wiley Periodicals LLC. FAU - Yi, Rule AU - Yi R AUID- ORCID: 0009-0007-6809-6837 AD - College of Grassland Science, Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization of Ministry of Agriculture and Rural Affairs, Key Laboratory of Grassland Resources, Ministry of Education Inner Mongolia Agricultural University Hohhot China. FAU - Hao, Junfeng AU - Hao J AUID- ORCID: 0000-0003-1283-2114 AD - College of Grassland Science, Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization of Ministry of Agriculture and Rural Affairs, Key Laboratory of Grassland Resources, Ministry of Education Inner Mongolia Agricultural University Hohhot China. FAU - Bao, Jian AU - Bao J AD - Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences Hohhot Inner Mongolia People's Republic of China. FAU - Zhao, Muqier AU - Zhao M AUID- ORCID: 0000-0001-8828-6151 AD - College of Grassland Science, Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization of Ministry of Agriculture and Rural Affairs, Key Laboratory of Grassland Resources, Ministry of Education Inner Mongolia Agricultural University Hohhot China. FAU - Wang, Zhijun AU - Wang Z AD - College of Grassland Science, Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization of Ministry of Agriculture and Rural Affairs, Key Laboratory of Grassland Resources, Ministry of Education Inner Mongolia Agricultural University Hohhot China. FAU - Bao, Zhuna AU - Bao Z AD - College of Grassland Science, Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization of Ministry of Agriculture and Rural Affairs, Key Laboratory of Grassland Resources, Ministry of Education Inner Mongolia Agricultural University Hohhot China. FAU - Ge, Gentu AU - Ge G AD - College of Grassland Science, Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization of Ministry of Agriculture and Rural Affairs, Key Laboratory of Grassland Resources, Ministry of Education Inner Mongolia Agricultural University Hohhot China. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Food Sci Nutr JT - Food science & nutrition JID - 101605473 PMC - PMC13714452 OTO - NOTNLM OT - Leontopodium OT - antioxidant activity OT - extraction methods OT - phenolic compounds OT - response surface methodology OT - ultrasound-assisted extraction COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/27 CRDT- 2026/10/29 05:31 PHST- 2026/03/31 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:31 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - FSN372523 [pii] AID - 10.1002/fsn3.72523 [doi] PST - epublish SO - Food Sci Nutr. 2026 Oct 27;14(11):e72523. doi: 10.1002/fsn3.72523. eCollection 2026 Nov. PMID- 42953033 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2054-5703 (Print) IS - 2054-5703 (Electronic) IS - 2054-5703 (Linking) VI - 13 IP - 10 DP - 2026 Oct 7 TI - The evolution of group size and kin structure in complex societies. PG - 260096 LID - 10.1098/rsos.260096 [doi] LID - 260096 AB - Molecular and pedigree data reveal substantial variation in genetic relatedness within social groups across animals. Yet kin selection theory typically treats relatedness as a fixed parameter rather than an emergent property of group dynamics. We develop a demographically explicit model in which group size fluctuates through births, dispersal, mortality and floater immigration. Residents and potential joiners resolve conflict over membership, with joining probabilities set by inclusive-fitness effects at demographic equilibrium. Membership control jointly shapes group size distributions, stability and relatedness. Under insider control, unrelated outsiders are admitted primarily to small, low-productivity groups, so mean relatedness rises with group size and stays high in larger groups. Groups are most stable near the per capita productivity optimum, with equilibrium sizes slightly above it. Low fecundity relative to resident mortality increases floater reliance, promoting mixed-kin groups and reducing relatedness. Under outsider control, groups grow larger by admitting unrelated floaters, and relatedness remains low. By treating kin structure as an emergent outcome of demographic processes and membership decisions rather than a fixed parameter, our framework links relatedness to the processes that form and regulate social groups, offering new insight into the evolution of sociality. CI - (c) 2026 The Authors. FAU - Shen, Sheng-Feng AU - Shen SF AUID- ORCID: 0000-0002-0631-6343 AD - Biodiversity Research Center, Academia Sinica, Taipei City, Taiwan. FAU - Rubenstein, Dustin R AU - Rubenstein DR AUID- ORCID: 0000-0002-4999-3723 AD - Department of Ecology, Evolution and Environmental Biology, Columbia University, New York, NY, USA. FAU - Liu, Ming AU - Liu M AUID- ORCID: 0000-0002-5170-8688 AD - Department of Biology, University of Oxford, Oxford, UK. LA - eng SI - figshare/10.6084/m9.figshare.c.8721452 PT - Journal Article DEP - 20261028 PL - England TA - R Soc Open Sci JT - Royal Society open science JID - 101647528 PMC - PMC13714408 OTO - NOTNLM OT - cooperative breeding OT - group size OT - inclusive fitness OT - insider-outsider conflict OT - kin structure COIS- We declare we have no competing interests. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/28 CRDT- 2026/10/29 05:30 PHST- 2026/01/15 00:00 [received] PHST- 2026/09/02 00:00 [revised] PHST- 2026/09/03 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:30 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - rsos260096 [pii] AID - 10.1098/rsos.260096 [doi] PST - epublish SO - R Soc Open Sci. 2026 Oct 28;13(10):260096. doi: 10.1098/rsos.260096. eCollection 2026 Oct 7. PMID- 42953027 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Omics-derived signatures in metabolic dysfunction-associated steatotic liver disease: from molecular characterization to clinical translation. PG - 1946657 LID - 10.3389/fmed.2026.1946657 [doi] LID - 1946657 AB - Metabolic dysfunction-associated steatotic liver disease (MASLD) is a highly prevalent systemic metabolic disorder with heterogeneous clinical trajectories. Although liver biopsy remains the histological reference standard, its invasiveness and limited scalability restrict routine use, while established non-invasive tests mainly capture fibrosis-related structural changes and provide limited insight into molecular heterogeneity, active steatohepatitis and early fibrotic transition. Multi-omics technologies offer a mechanistically informed framework for resolving this heterogeneity. In this Review, we summarize recent advances in genomic, transcriptomic, single-cell and spatial, proteomic, lipidomic and metabolomic signatures and candidate biomarkers in MASLD. Genomic studies define inherited susceptibility and risk enrichment, whereas transcriptomic and spatial approaches reveal disease-associated pathways, cell states and tissue niches. Proteomics provides blood-accessible signals of steatohepatitis activity, matrix remodelling and systemic metabolic risk, while lipidomics and metabolomics capture lipotoxic lipid composition, mitochondrial stress, bile acid dysregulation and gut-liver metabolic perturbation. We further discuss how integrative multi-omics, systems biology and artificial intelligence can organize high-dimensional molecular data into disease modules, molecular subtypes, biomarker signatures and candidate therapeutic nodes. In addition, omics-informed studies of bioactive compounds and precision nutrition illustrate how nutritional modulation may be evaluated through molecular response profiles. Despite promising progress, most omics-derived biomarkers remain limited by cohort specificity, platform dependence, incomplete external validation and unclear clinical thresholds. Future translation will require longitudinal, spatially resolved and clinically anchored integration to support risk stratification, at-risk metabolic dysfunction-associated steatohepatitis detection, fibrosis assessment, treatment-response prediction and precision medicine in MASLD. CI - (c) 2026 Qin, Juramt, Chen, Li, Wei, Yang, Chen and Qiu. FAU - Qin, Ziyang AU - Qin Z AD - Department of Hepatobiliary and Pancreatic Surgery, General Surgery Center, the First Hospital of Jilin University, Changchun, Jilin, China. FAU - Juramt, Dulguun AU - Juramt D AD - Department of Hepatology and Gastroenterology, Charite-Universitatsmedizin Berlin, Campus Virchow-Klinikum (CVK) and Campus Charite Mitte (CCM), Berlin, Germany. FAU - Chen, Yuguo AU - Chen Y AD - Department of Hepatobiliary and Pancreatic Surgery, General Surgery Center, the First Hospital of Jilin University, Changchun, Jilin, China. FAU - Li, Yunyi AU - Li Y AD - Department of Hepatobiliary and Pancreatic Surgery, General Surgery Center, the First Hospital of Jilin University, Changchun, Jilin, China. FAU - Wei, Jiaqi AU - Wei J AD - Department of Hepatobiliary and Pancreatic Surgery, General Surgery Center, the First Hospital of Jilin University, Changchun, Jilin, China. FAU - Yang, Hongyu AU - Yang H AD - Department of Hepatobiliary and Pancreatic Surgery, General Surgery Center, the First Hospital of Jilin University, Changchun, Jilin, China. FAU - Chen, Lanlan AU - Chen L AD - Department of Hepatobiliary and Pancreatic Surgery, General Surgery Center, the First Hospital of Jilin University, Changchun, Jilin, China. AD - Department of Hepatology and Gastroenterology, Charite-Universitatsmedizin Berlin, Campus Virchow-Klinikum (CVK) and Campus Charite Mitte (CCM), Berlin, Germany. FAU - Qiu, Wei AU - Qiu W AD - Department of Hepatobiliary and Pancreatic Surgery, General Surgery Center, the First Hospital of Jilin University, Changchun, Jilin, China. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13714508 OTO - NOTNLM OT - biomarkers OT - metabolic dysfunction-associated steatohepatitis OT - metabolic dysfunction-associated steatotic liver disease OT - metabolomics OT - multi-omics OT - precision medicine OT - proteomics OT - spatial transcriptomics COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author LC declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:30 PHST- 2026/07/23 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:30 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmed.2026.1946657 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 14;13:1946657. doi: 10.3389/fmed.2026.1946657. eCollection 2026. PMID- 42953025 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2398-8835 (Electronic) IS - 2398-8835 (Linking) VI - 9 IP - 11 DP - 2026 Nov TI - Investigating the Effect of Photovoice on the Resilience and Perceived Social Stigma of Patients With Type 1 Diabetes: A Study Protocol for a Randomized Controlled Trial. PG - e72939 LID - 10.1002/hsr2.72939 [doi] LID - e72939 AB - BACKGROUND AND AIMS: Patients with type 1 diabetes often experience high levels of perceived social stigma, which can severely impact their psychological resilience. Photovoice is an innovative visual participatory method, but its efficacy in this population remains unexplored. This study protocol aims to evaluate the effect of a photovoice intervention on resilience and perceived social stigma in patients with type 1 diabetes. METHODS: This is a single-blind, two-group, three-stage clinical trial with a pretest-posttest design and 1-month follow-up. A total of 56 patients with type 1 diabetes will be selected fromIsfahan Comprehensive Health Centers using cluster sampling before being divided into two intervention and control groups by a simple randomization method. The intervention group will undergo a 4-week photovoice intervention, which focuses on topics such as identity recovery, management of fear and negative emotions, compatibility with family and friends, and management of emotions resulting from judgments and blame. During the intervention, participants will prepare photos with specified topics and analyze and discuss them in group sessions using the SHOWED technique. The control group will receive routine care at the health centers. Resilience and perceived social stigma in both groups will be measured before, immediately after, and 1 month after the intervention using standard questionnaires. The data will be analyzed through SPSS-20 software using t-tests and repeated measures ANOVA. RESULTS: Results will be reported in accordance with CONSORT guidelines following the completion of the trial. This paper presents the rationale and methodology for the study. CONCLUSION: This study will evaluate the feasibility and effectiveness of the photovoice intervention in enhancing psychological resilience and reducing perceived social stigma in patients with type 1 diabetes, and will provide evidence for the application of this approach in nursing care. CI - (c) 2026 The Author(s). Health Science Reports published by Wiley Periodicals LLC. FAU - Taheri Tehrani, Samaneh AU - Taheri Tehrani S AUID- ORCID: 0009-0006-3606-8412 AD - Department of Nursing, Nursing and Midwifery School Isfahan University of Medical Sciences Isfahan Iran. FAU - Mardanian Dehkordi, Leila AU - Mardanian Dehkordi L AUID- ORCID: 0000-0001-8250-8120 AD - Nursing and Midwifery Care Research Center Isfahan University of Medical Sciences Isfahan Iran. FAU - Elahdadi, Sajjad AU - Elahdadi S AD - Department of Assessment and Measurement, Faculty of Psychology and Educational Sciences Allameh Tabataba'i University Tehran Iran. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Health Sci Rep JT - Health science reports JID - 101728855 PMC - PMC13714449 OTO - NOTNLM OT - perceived social stigma OT - photovoice OT - resilience OT - type 1 diabetes COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/27 CRDT- 2026/10/29 05:30 PHST- 2025/07/22 00:00 [received] PHST- 2026/07/17 00:00 [revised] PHST- 2026/07/24 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:30 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - HSR272939 [pii] AID - 10.1002/hsr2.72939 [doi] PST - epublish SO - Health Sci Rep. 2026 Oct 27;9(11):e72939. doi: 10.1002/hsr2.72939. eCollection 2026 Nov. PMID- 42953017 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-634X (Print) IS - 2296-634X (Electronic) IS - 2296-634X (Linking) VI - 14 DP - 2026 TI - Research progress on the BAG3-HSP70 axis-mediated autophagy in acute myeloid leukemia. PG - 1980188 LID - 10.3389/fcell.2026.1980188 [doi] LID - 1980188 AB - Acute myeloid leukemia (AML) is a genetically heterogeneous hematological malignancy in which chemoresistance and relapse, often driven by leukemia stem cells (LSCs), remain major obstacles to cure. Autophagy is an evolutionarily conserved lysosomal degradation pathway whose function in AML is not fixed but is stage- and context-dependent: it restrains leukemogenesis in some settings while sustaining established and therapy-stressed LSCs in others. The Bcl-2-associated athanogene 3 (BAG3)-heat shock protein 70 (HSP70) axis integrates proteotoxic stress with the autophagy machinery, most notably through chaperone-assisted selective autophagy (CASA). This review applies a four-tier evidence framework-direct AML evidence, other hematological evidence, non-AML evidence, and proposed or hypothetical mechanisms-and explicitly separates BAG3-specific evidence from general HSP70 and autophagy findings. Direct AML evidence is comparatively strong for the cytoprotective, mitophagic and chemoresistance roles of autophagy and HSP70, but it is limited for a specific BAG3 dependency. In particular, direct BAG3 coupling to ULK1, competitive BAG3/HSP70 binding at the Beclin1 BH3 domain, and direct HSP70 shielding of ATG5 from cleavage are not supported by primary evidence. We examine the crosstalk between this axis and recurrent AML alterations (FLT3-ITD, NPM1 and TP53), and survey therapeutic strategies ranging from ATP-site HSP70 inhibitors to HSP70-BAG3 protein-protein interaction inhibitors, dual HSP70/HSP90 targeting, BAG3-directed agents, targeted protein degradation, nanoparticle delivery and emerging immunotherapies. We stratify direct axis-targeting from broad HSP70-family or proteostasis approaches. Finally, we summarize the principal knowledge gaps and the potential therapeutic window, cardiac-safety and biomarker challenges that must be addressed to convert the BAG3-HSP70 axis from a plausible mechanism into a precision target in AML. CI - Copyright (c) 2026 Liu and Wu. FAU - Liu, Yang AU - Liu Y AD - Department of Hematology, People's Liberation Army Joint Logistics Support Force 940th Hospital, Lanzhou, China. FAU - Wu, Tao AU - Wu T AD - Department of Hematology, People's Liberation Army Joint Logistics Support Force 940th Hospital, Lanzhou, China. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Cell Dev Biol JT - Frontiers in cell and developmental biology JID - 101630250 PMC - PMC13714359 OTO - NOTNLM OT - BAG3 OT - HSP70 OT - acute myeloid leukemia OT - autophagy OT - chaperone-assisted selective autophagy OT - chemoresistance OT - leukemia stem cells OT - targeted therapy COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:29 PHST- 2026/08/27 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:29 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1980188 [pii] AID - 10.3389/fcell.2026.1980188 [doi] PST - epublish SO - Front Cell Dev Biol. 2026 Oct 14;14:1980188. doi: 10.3389/fcell.2026.1980188. eCollection 2026. PMID- 42953014 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1663-4365 (Print) IS - 1663-4365 (Electronic) IS - 1663-4365 (Linking) VI - 18 DP - 2026 TI - A clinical prediction model for 90-day functional dependence after minor stroke/TIA with exploratory pharmacogenetic associations: a post-hoc analysis of the INSPIRES trial. PG - 1922100 LID - 10.3389/fnagi.2026.1922100 [doi] LID - 1922100 AB - BACKGROUND AND PURPOSE: Dual antiplatelet therapy (DAPT) reduces stroke recurrence after minor stroke/TIA, yet a clinically meaningful proportion of patients remain functionally dependent at 90 days. We developed an interpretable clinical prediction model to define a high-risk subgroup, and separately explored pharmacogenetic associations within this subgroup to generate hypotheses for precision secondary prevention. METHODS: In this post-hoc analysis of the INSPIRES trial (4,439 patients), LASSO logistic regression selected predictors for 90-day functional dependence (mRS > 2). Model performance was assessed by discrimination, calibration, and decision curve analysis. In a genotyped subcohort, CYP1A2 rs762551 and POR rs1057868 were evaluated for outcome association and enrichment in the high-risk subgroup. RESULTS: Logistic regression achieved the highest AUC point estimate (AUC = 0.727) among the four models evaluated (AUC range: 0.495-0.698; 95% CIs overlapped across models); it was selected as the final model for its interpretability and calibration, rather than for statistical superiority. Key predictors were admission NIHSS, age >65 years, and diabetes. The highest-risk quartile (25% of patients) carried a disproportionate outcome burden, with no effect modification by antiplatelet strategy (p = 0.793). CYP1A2 CC (23.65% vs. 16.28%; Pearson chi (2) p = 0.0031) and POR TC (54.58% vs. 46.22%; Pearson chi (2) p = 0.0105) genotypes were enriched in functionally dependent patients; CYP1A2 showed no significant allele-dosage trend (trend p = 0.0821), whereas POR demonstrated a significant allele-dosage trend (p = 0.0032). CONCLUSION: This clinical prediction model identified a high-risk subgroup accounting for most poor outcomes after minor stroke/TIA. Exploratory analyses identified CYP1A2 and POR variants enriched in this subgroup, generating pharmacogenetic hypotheses for future investigation. CI - Copyright (c) 2026 Wang, Li, Yan, Jiang, Pan and Wang. FAU - Wang, Yaping AU - Wang Y AD - Beijing Tiantan Hospital, Capital Medical University, Beijing, China. FAU - Li, Baizhu AU - Li B AD - Beijing Tiantan Hospital, Capital Medical University, Beijing, China. FAU - Yan, Hongyi AU - Yan H AD - Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, China. FAU - Jiang, Lingling AU - Jiang L AD - China National Clinical Research Center for Neurological Diseases, Beijing, China. FAU - Pan, Yuesong AU - Pan Y AD - Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, China. FAU - Wang, Yilong AU - Wang Y AD - Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, China. AD - Chinese Institute for Brain Research, Beijing, China. AD - National Center for Neurological Disorders, Beijing, China. AD - Advanced Innovation Center for Human Brain Protection, Capital Medical University, Beijing, China. AD - Beijing Laboratory of Oral Health, Capital Medical University, Beijing, China. AD - Laboratory for Clinical Medicine, Capital Medical University, Beijing, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Aging Neurosci JT - Frontiers in aging neuroscience JID - 101525824 PMC - PMC13713692 OTO - NOTNLM OT - dual antiplatelet therapy (DAPT) OT - functional outcome OT - minor stroke OT - pharmacogenetics OT - prediction model OT - risk stratification OT - transient ischemic attack (TIA) COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:29 PHST- 2026/06/28 00:00 [received] PHST- 2026/08/27 00:00 [revised] PHST- 2026/09/07 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:29 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fnagi.2026.1922100 [doi] PST - epublish SO - Front Aging Neurosci. 2026 Oct 14;18:1922100. doi: 10.3389/fnagi.2026.1922100. eCollection 2026. PMID- 42953008 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2673-7647 (Electronic) IS - 2673-7647 (Linking) VI - 6 DP - 2026 TI - In silico design of novel indolinone-based anticancer agents and proposal of synthetic routes. PG - 1964610 LID - 10.3389/fbinf.2026.1964610 [doi] LID - 1964610 AB - The RAF-MEK-ERK signaling pathway plays a central role in cancer development and progression and represents an important target for anticancer drug discovery. Simultaneous targeting of components of this pathway may therefore represent a promising strategy for cancer treatment. A quantitative structure-activity relationship (QSAR) model was developed using multiple linear regression (MLR). The QSAR model showed satisfactory predictive performance based on complementary internal and external validation metrics. The selected molecular descriptors were used to guide the structural modification of substituents, resulting in higher predicted activity. Accordingly, ten new molecules were designed through strategic substituent modifications. Synthetic routes for these compounds were also proposed based on reported literature procedures. Molecular docking, molecular dynamics (MD) simulations, and Petra, Osiris, and Molinspiration (POM) analyses supported the prioritization of Pred 9 and Pred 10 as computationally designed candidates for further investigation. In silico absorption, distribution, metabolism, excretion, and toxicity (ADMET) predictions indicated that Pred 9 and Pred 10 may exhibit favorable pharmacokinetic properties. Overall, the results support the consideration of these compounds for experimental synthesis and biological evaluation. However, Pred 9 and Pred 10 have not yet been synthesized or experimentally evaluated for MEK1 inhibition or anticancer activity. CI - Copyright (c) 2026 Er-rajy, El Fadili, Etabti, Aziz, Lenda, Mujwar, Chtita, Samadi and Elhallaoui. FAU - Er-Rajy, Mohammed AU - Er-Rajy M AD - LIMAS Laboratory, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez, Morocco. FAU - El Fadili, Mohamed AU - El Fadili M AD - LIMAS Laboratory, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez, Morocco. FAU - Etabti, Hanane AU - Etabti H AD - LIMAS Laboratory, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez, Morocco. FAU - Aziz, Arzine AU - Aziz A AD - Engineering Laboratory of Organometallic, Molecular Materials, and Environment (LIMOME). Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez, Morocco. FAU - Lenda, Fatimazohra AU - Lenda F AD - Physical-Chemical Laboratory of Materials, Natural Substances and the Environment (LAMSE), Department of Chemistry, Faculty of Sciences, Techniques of Tangier-University Abdelmalek Essaadi, Tangier, Morocco. FAU - Mujwar, Somdutt AU - Mujwar S AD - Chitkara College of Pharmacy, Chitkara University, Rajpura, Punjab, India. FAU - Chtita, Samir AU - Chtita S AD - Laboratory of Analytical and Molecular Chemistry, Faculty of Sciences Ben M'Sik, Hassan II University of Casablanca, Casablanca, Morocco. FAU - Samadi, Abdelouahid AU - Samadi A AD - Department of Chemistry, College of Science, United Arab Emirates University, Al Ain, United Arab Emirates. FAU - Elhallaoui, Menana AU - Elhallaoui M AD - LIMAS Laboratory, Faculty of Sciences Dhar El Mahraz, Sidi Mohamed Ben Abdellah University, Fez, Morocco. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Bioinform JT - Frontiers in bioinformatics JID - 9918227263306676 PMC - PMC13713695 OTO - NOTNLM OT - POM analysis OT - QSAR method OT - anticancer agents OT - indolinone OT - molecular docking OT - molecular dynamics OT - synthetic routes COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:29 PHST- 2026/08/11 00:00 [received] PHST- 2026/09/07 00:00 [revised] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:29 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1964610 [pii] AID - 10.3389/fbinf.2026.1964610 [doi] PST - epublish SO - Front Bioinform. 2026 Oct 14;6:1964610. doi: 10.3389/fbinf.2026.1964610. eCollection 2026. PMID- 42953006 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1663-9812 (Print) IS - 1663-9812 (Electronic) IS - 1663-9812 (Linking) VI - 17 DP - 2026 TI - CT habitat and peritumoral radiomics for predicting progression after repeat TACE in hepatocellular carcinoma: a multicenter study. PG - 1942002 LID - 10.3389/fphar.2026.1942002 [doi] LID - 1942002 AB - BACKGROUND: This study developed and validated an machine-learning-based radiomics model integrating tumor habitat radiomics, peritumoral radiomics, and clinical features to predict progression-free survival (PFS) following repeat transarterial chemoembolization (TACE) in patients with hepatocellular carcinoma (HCC). METHODS: This retrospective multicenter study included 270 patients with HCC. Patients from Center 1 were assigned to training (n = 94) and internal validation (n = 41) cohorts, while Centers 2 (n = 82) and 3 (n = 53) served as external validation cohorts. Tumor habitats and 5-mm peritumoral regions were generated from contrast-enhanced CT images. Radiomics features were selected using univariate Cox and LASSO-Cox regression. A fusion model and HPC risk score were constructed by integrating selected radiomics signatures and clinical variables. Performance was assessed using time-dependent ROC, C-index, calibration, decision curve analysis, and Kaplan-Meier analyses. RESULTS: The optimal habitat cluster number was K = 2. Eleven habitat features, three peritumoral features, and alpha-fetoprotein were retained in the fusion model. The model achieved AUCs of 0.923, 0.921, and 0.897 for 3-, 6-, and 9-month progression-free survival in the training cohort, and 0.932, 0.863, and 0.916 in the internal validation cohort, respectively; all external AUCs exceeded 0.82. The fusion model showed higher C-index values than the clinical model across cohorts. High-risk patients had significantly shorter PFS than low-risk patients (all P < 0.001). Calibration and decision curve analyses demonstrated good agreement and clinical utility. CONCLUSION: The CT-based fusion model and HPC risk score effectively predicted progression risk in HCC. CI - Copyright (c) 2026 Wang, Hu, Shi, He, Sui, Wen, Zhan, Yang, Han, Wang, Yang, Lv, Liu and Cui. FAU - Wang, Fei AU - Wang F AD - Department of Interventional Radiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. FAU - Hu, Yue AU - Hu Y AD - Department of Central Sterile Supply, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. FAU - Shi, Zhong-Xing AU - Shi ZX AD - Department of Interventional Radiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. FAU - He, Xin-Yu AU - He XY AD - Department of Interventional Radiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. FAU - Sui, Yu-Hang AU - Sui YH AD - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. FAU - Wen, Fu-Kai AU - Wen FK AD - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. FAU - Zhan, Jia-Shan AU - Zhan JS AD - Department of Radiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China. FAU - Yang, Ning-Jing AU - Yang NJ AD - Department of Radiology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China. FAU - Han, Xue-Jing AU - Han XJ AD - Department of Interventional Radiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. FAU - Wang, Jia-Hui AU - Wang JH AD - Department of Interventional Radiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. FAU - Yang, Jia-Yi AU - Yang JY AD - Department of Interventional Radiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. FAU - Lv, Wen AU - Lv W AD - Department of Interventional Radiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. FAU - Liu, Hong-Fei AU - Liu HF AD - Department of Interventional Radiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. FAU - Cui, Li-Ming AU - Cui LM AD - Department of Interventional Radiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Pharmacol JT - Frontiers in pharmacology JID - 101548923 PMC - PMC13713701 OTO - NOTNLM OT - artificial intelligence OT - habitat radiomics OT - hepatocellular carcinoma OT - peritumoral radiomics OT - transarterial chemoembolization COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:29 PHST- 2026/07/19 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:29 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1942002 [pii] AID - 10.3389/fphar.2026.1942002 [doi] PST - epublish SO - Front Pharmacol. 2026 Oct 14;17:1942002. doi: 10.3389/fphar.2026.1942002. eCollection 2026. PMID- 42953002 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1663-9812 (Print) IS - 1663-9812 (Electronic) IS - 1663-9812 (Linking) VI - 17 DP - 2026 TI - Risk prediction models for prolonged postoperative opioid use: a systematic review and meta-analysis of drug-risk stratification, validation and evidence benchmarks. PG - 1946700 LID - 10.3389/fphar.2026.1946700 [doi] LID - 1946700 AB - Prolonged postoperative opioid use represents a medication-safety problem in which short-term mu-opioid receptor agonist exposure may transition to sustained consumption, tolerance, opioid-induced hyperalgesia and dependence in susceptible patients. Risk prediction models could support clinical pharmacology-informed opioid stewardship by identifying patients who require individualised dosing, closer follow-up, alternative analgesic strategies or deprescribing support. We searched PubMed/MEDLINE, Embase, Web of Science, Scopus and the Cochrane Library from inception to 5 July 2026 for studies developing or validating multivariable models predicting opioid use beyond 30 days after surgery. One primary estimate was selected per independent cohort. Area under the receiver operating characteristic curve (AUROC) values were logit-transformed and pooled using random-effects restricted maximum likelihood models with Hartung-Knapp confidence intervals. 35 reports met the eligibility criteria and contributed 35 independent AUROC estimates to the quantitative synthesis. The pooled AUROC was 0.794 (95% CI 0.751-0.832), although between-study heterogeneity was extreme (I(2) = 99%; 95% prediction interval, 0.512-0.934). Exploratory analyses suggested numerically lower discrimination in externally or temporally validated models and in opioid-naive cohorts, but these findings were not consistently supported by meta-regression. Current models identify a measurable drug-risk signal, yet few meet all four predefined translational-evidence benchmarks, and insufficient validation, calibration and clinical-utility evidence limits their immediate use for individualised opioid pharmacotherapy. SYSTEMATIC REVIEW REGISTRATION: https://www.crd.york.ac.uk/PROSPERO/view/CRD420261442043, identifier CRD420261442043. CI - Copyright (c) 2026 Liao, Hou, Song, Yu, Li, Lu, Zheng and Chen. FAU - Liao, Yin-Sheng AU - Liao YS AD - Department of Anesthesiology, Zigong Fourth People's Hospital, Zigong, Sichuan, China. FAU - Hou, Ping AU - Hou P AD - Department of Anesthesiology, Zigong Fourth People's Hospital, Zigong, Sichuan, China. FAU - Song, Jian-Li AU - Song JL AD - Department of Anesthesiology, Zigong Fourth People's Hospital, Zigong, Sichuan, China. FAU - Yu, Xuan AU - Yu X AD - Department of Anesthesiology, Zigong Fourth People's Hospital, Zigong, Sichuan, China. FAU - Li, Qiang AU - Li Q AD - Department of Anesthesiology, Zigong Fourth People's Hospital, Zigong, Sichuan, China. FAU - Lu, Bin AU - Lu B AD - Department of Anesthesiology, Zigong Fourth People's Hospital, Zigong, Sichuan, China. FAU - Zheng, Mao-Yao AU - Zheng MY AD - Department of Anesthesiology, Zigong First People's Hospital, Zigong Academy of Medical Sciences, Zigong, Sichuan, China. FAU - Chen, Guan-Yu AU - Chen GY AD - Department of Anesthesiology, Zigong Fourth People's Hospital, Zigong, Sichuan, China. LA - eng PT - Journal Article PT - Systematic Review DEP - 20261014 PL - Switzerland TA - Front Pharmacol JT - Frontiers in pharmacology JID - 101548923 PMC - PMC13713708 OTO - NOTNLM OT - drug-risk stratification OT - opioid stewardship OT - prediction model OT - prolonged postoperative opioid use OT - systematic review COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:29 PHST- 2026/07/23 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:29 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1946700 [pii] AID - 10.3389/fphar.2026.1946700 [doi] PST - epublish SO - Front Pharmacol. 2026 Oct 14;17:1946700. doi: 10.3389/fphar.2026.1946700. eCollection 2026. PMID- 42953000 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1663-9812 (Print) IS - 1663-9812 (Electronic) IS - 1663-9812 (Linking) VI - 17 DP - 2026 TI - Extrachromosomal circular DNA drives focal amplification of AGAP2-AS1 to promote cutaneous tumorigenesis. PG - 1906797 LID - 10.3389/fphar.2026.1906797 [doi] LID - 1906797 AB - BACKGROUND: Extrachromosomal circular DNA (eccDNA) represents a pervasive structural continuum of genomic instability that drives intratumoral heterogeneity; the topography of the eccDNA-encoded non-coding transcriptome remains obscure in ultraviolet (UV)-driven malignancies. METHODS: We profiled eccDNA from Basal Cell Carcinoma, Inverted Follicular Keratosis, Merkel Cell Carcinoma, and matched adjacent normal tissues using Circle-Seq. Structural data were integrated with the eccDNA Gene Analyzer (ECGA) and The Cancer Genome Atlas (TCGA) clinical cohorts. In vitro siRNA-mediated knockdown assays were used to determine the oncogenic functionality of identified lncRNAs. RESULTS: Circle-Seq revealed genome-wide remodeling of circular DNA with focal hyper-amplification, GC-rich compositional biases, and recurrent hotspots on chromosomes 9, 16, and 20. Integrative screening identified AGAP2-AS1 as a clinically significant, copy-number-associated lncRNA, whose expression correlated with cellular responses to UV radiation and oxidative stress. Depletion of AGAP2-AS1 impaired tumor cell proliferation, migration, and invasion in vitro. CONCLUSION: The eccDNA landscape serves as a reservoir of functional non-coding elements; focal amplification of AGAP2-AS1 contributes to malignant progression and stress adaptation in cutaneous neoplasia. CI - Copyright (c) 2026 Zhang, Liu, Zhao, Liu, Liu, Xue, Lin and Zhu. FAU - Zhang, Jin AU - Zhang J AD - Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin, China. AD - Department of Ophthalmology, The First Hospital of Hohhot, Hohhot, China. FAU - Liu, Zeying AU - Liu Z AD - Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin, China. FAU - Zhao, Jinzhi AU - Zhao J AD - Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin, China. FAU - Liu, Xun AU - Liu X AD - Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin, China. FAU - Liu, Zhiying AU - Liu Z AD - Department of Ophthalmology, The First Hospital of Hohhot, Hohhot, China. FAU - Xue, Huiting AU - Xue H AD - Tumor Multimodal Digital and Intelligent Precision Diagnosis Engineering Research Center, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot, China. FAU - Lin, Tingting AU - Lin T AD - Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin, China. FAU - Zhu, Limin AU - Zhu L AD - Tianjin Key Laboratory of Retinal Functions and Diseases, Tianjin Branch of National Clinical Research Center for Ocular Disease, Eye Institute and School of Optometry, Tianjin Medical University Eye Hospital, Tianjin, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Pharmacol JT - Frontiers in pharmacology JID - 101548923 PMC - PMC13713694 OTO - NOTNLM OT - AGAP2-AS1 OT - cutaneous tumorigenesis OT - extrachromosomal circular DNA OT - eyelid neoplasm OT - gene amplification COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:28 PHST- 2026/06/11 00:00 [received] PHST- 2026/08/06 00:00 [revised] PHST- 2026/08/17 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:28 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1906797 [pii] AID - 10.3389/fphar.2026.1906797 [doi] PST - epublish SO - Front Pharmacol. 2026 Oct 14;17:1906797. doi: 10.3389/fphar.2026.1906797. eCollection 2026. PMID- 42952994 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1662-5161 (Print) IS - 1662-5161 (Electronic) IS - 1662-5161 (Linking) VI - 20 DP - 2026 TI - Advances in artificial intelligence for brain imaging and computer-aided diagnosis of neurological diseases: a systematic literature review. PG - 1963855 LID - 10.3389/fnhum.2026.1963855 [doi] LID - 1963855 AB - BACKGROUND: Brain imaging is central to the structural, functional, metabolic, and neurophysiological characterization of neurological disease, yet interpretation remains constrained by heterogeneity in acquisition, annotation, expertise, and clinical context. OBJECTIVE: This systematic literature review evaluates recent artificial intelligence approaches for brain imaging and computer-aided diagnosis, with emphasis on methods, modalities, neurological diseases, diagnostic tasks, datasets, validation practices, and translational limitations. METHODS: The review followed PRISMA 2020 and covered peer-reviewed English-language studies published from January 2020 through June 2026. Eight information sources were searched using database-specific terms for artificial intelligence, neuroimaging, neurological disease, and diagnostic analysis. The search identified 1,078 records; 236 duplicates were removed, 842 unique records were screened, 258 full texts were assessed, and 123 studies were included after a record-level eligibility re-audit. Heterogeneous evidence was synthesized narratively and by descriptive mapping rather than meta-analysis. RESULTS: MRI was frequently represented in the included studies and auxiliary mapping data; CT was commonly used in acute stroke and intracranial hemorrhage; PET supported selected molecular and differential-diagnosis studies; fMRI and diffusion imaging were used for network and white-matter analysis; and EEG extended the scope to neurophysiological diagnosis. CNN and three-dimensional networks were commonly reported, while attention, Transformer, graph, self-supervised, generative, multimodal, and foundation-model approaches appeared in selected recent studies. Stroke, dementia, and brain tumors were frequently represented, but heterogeneous populations, outcomes, and incomplete validation extraction precluded ranking diseases, modalities, architectures, or validation strategies. CONCLUSION: The evidence suggests movement from task-specific pattern recognition toward multimodal and context-rich decision support in selected settings, but it does not establish uniform methodological superiority or clinical maturity. Dependable translation requires patient-level data governance, transparent reporting, uncertainty estimation, independent multicenter testing, prospective workflow evaluation, and clinically meaningful endpoints. CI - Copyright (c) 2026 Omarov. FAU - Omarov, Batyrkhan AU - Omarov B AD - School of Digital Technologies, Narxoz University, Almaty, Kazakhstan. AD - Department of Mathematical and Computer Modeling, International Information Technology University, Almaty, Kazakhstan. AD - Department of Software Engineering, Faculty of Physics, Mathematics and Information Technology, Khalel Dosmukhamedov Atyrau University, Atyrau, Kazakhstan. LA - eng PT - Journal Article PT - Systematic Review DEP - 20261014 PL - Switzerland TA - Front Hum Neurosci JT - Frontiers in human neuroscience JID - 101477954 PMC - PMC13713707 OTO - NOTNLM OT - artificial intelligence OT - brain imaging OT - computer-aided diagnosis OT - deep learning OT - magnetic resonance imaging OT - neuroimaging OT - neurological diseases OT - systematic review COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:28 PHST- 2026/08/10 00:00 [received] PHST- 2026/09/01 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:28 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fnhum.2026.1963855 [doi] PST - epublish SO - Front Hum Neurosci. 2026 Oct 14;20:1963855. doi: 10.3389/fnhum.2026.1963855. eCollection 2026. PMID- 42952983 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2297-1769 (Print) IS - 2297-1769 (Electronic) IS - 2297-1769 (Linking) VI - 13 DP - 2026 TI - CircRNA circRtn4 as a candidate molecular marker for postmortem interval estimation in mouse brain tissues. PG - 1941625 LID - 10.3389/fvets.2026.1941625 [doi] LID - 1941625 AB - INTRODUCTION: Estimation of the postmortem interval (PMI) remains a major challenge in forensic research. Traditional PMI estimation methods using postmortem changes were usually inaccurate or empirical. Owing to their stability, abundance, and tissue specificity, circular RNAs (circRNAs) are promising candidates for PMI estimation. This study explores circRNA markers and aims to develop PMI estimation models in mouse brain tissue, with exploratory modeling in human samples. METHODS: Candidate circRNAs and reference genes were selected from online databases, and primers were designed accordingly. The back-splice junctions of candidate circRNAs were detected by Sanger sequencing. Total RNA was extracted from mouse and human samples collected across different PMIs and temperatures. Semi-quantitative RT-PCR and RT-qPCR were used to quantify circRNAs' levels, followed by linear and nonlinear regression modeling. Predictive accuracy was assessed using validation samples. RESULTS: At 25  degrees C, mRNA degraded faster than the other tested RNAs. According to postmortem stability, 28S rRNA, mt-co1, and circCDR1as were selected as reference genes for the PMI estimation model at room temperature, and 28S rRNA and mt-co1 were selected as reference genes at high temperature. CircRtn4 exhibited PMI-associated changes under both experimental conditions. In semi-quantitative RT-PCR experiments, model comparison supported quadratic models for the 25  degrees C data normalized to 28S rRNA and circCDR1as, whereas linear models were favored for mt-co1 at 25  degrees C and for both internal reference at 35  degrees C. Additionally, cubic terms provided no additional benefit. Validation using known-PMI samples showed variable prediction errors across models and time points. In the RT-qPCR analysis at 25  degrees C, only the linear model showed a statistically significant relationship with PMI. Exploratory regression equations were fitted to the single-donor human data at 20  degrees C and 25  degrees C. DISCUSSION: CircRtn4 may serve as a molecular marker for PMI estimation in mouse brain tissue under the tested temperature conditions. These findings indicate that postmortem circRNA-based models should be developed under defined temperature and species conditions. Further independent validation in veterinary-relevant species and human cohorts is required before considering forensic application. CI - Copyright (c) 2026 Song, Qian, Fu, Xiang, Lu, Zhou, Yang, Cheng and Fu. FAU - Song, Binghui AU - Song B AD - Key Laboratory of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Southwest Medical University, Luzhou, Sichuan, China. AD - Laboratory of Precision Medicine and DNA Forensic Medicine, the Research Center for Preclinical Medicine, Southwest Medical University, Luzhou, Sichuan, China. FAU - Qian, Jie AU - Qian J AD - Key Laboratory of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Southwest Medical University, Luzhou, Sichuan, China. FAU - Fu, Jiewen AU - Fu J AD - Key Laboratory of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Southwest Medical University, Luzhou, Sichuan, China. AD - Laboratory of Precision Medicine and DNA Forensic Medicine, the Research Center for Preclinical Medicine, Southwest Medical University, Luzhou, Sichuan, China. AD - Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand. FAU - Xiang, Zhuohang AU - Xiang Z AD - Key Laboratory of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Southwest Medical University, Luzhou, Sichuan, China. AD - Laboratory of Precision Medicine and DNA Forensic Medicine, the Research Center for Preclinical Medicine, Southwest Medical University, Luzhou, Sichuan, China. FAU - Lu, Lin AU - Lu L AD - Key Laboratory of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Southwest Medical University, Luzhou, Sichuan, China. AD - Laboratory of Precision Medicine and DNA Forensic Medicine, the Research Center for Preclinical Medicine, Southwest Medical University, Luzhou, Sichuan, China. FAU - Zhou, Tiejun AU - Zhou T AD - Department of Pathology, the Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China. FAU - Yang, Lisha AU - Yang L AD - Department of Obstetrics and Center for Prenatal Diagnosis, The Affiliated Hospital of Southwest Medical University, Luzhou, China. FAU - Cheng, Jingliang AU - Cheng J AD - Key Laboratory of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Southwest Medical University, Luzhou, Sichuan, China. AD - Laboratory of Precision Medicine and DNA Forensic Medicine, the Research Center for Preclinical Medicine, Southwest Medical University, Luzhou, Sichuan, China. FAU - Fu, Junjiang AU - Fu J AD - Key Laboratory of Epigenetics and Oncology, The Research Center for Preclinical Medicine, Southwest Medical University, Luzhou, Sichuan, China. AD - Laboratory of Precision Medicine and DNA Forensic Medicine, the Research Center for Preclinical Medicine, Southwest Medical University, Luzhou, Sichuan, China. AD - Laboratory of Forensic DNA, the Judicial Authentication Center, Southwest Medical University, Luzhou, Sichuan, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Vet Sci JT - Frontiers in veterinary science JID - 101666658 PMC - PMC13714255 OTO - NOTNLM OT - circular RNA (circRNA) OT - forensic biomarkers OT - postmortem RNA degradation OT - postmortem interval (PMI) OT - veterinary forensic science COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:27 PHST- 2026/07/18 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:27 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fvets.2026.1941625 [doi] PST - epublish SO - Front Vet Sci. 2026 Oct 14;13:1941625. doi: 10.3389/fvets.2026.1941625. eCollection 2026. PMID- 42952981 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2674-0702 (Electronic) IS - 2674-0702 (Linking) VI - 6 DP - 2026 TI - One health perspectives on pharmaceutical and clinical effluents driving multidrug resistance in aquatic ecosystems: a comprehensive review. PG - 1890380 LID - 10.3389/fsysb.2026.1890380 [doi] LID - 1890380 AB - Antimicrobial resistance (AMR) has emerged as one of the most pressing global health challenges, yet the contribution of environmental reservoirs to the spread of multidrug resistance (MDR) remains insufficiently integrated into mainstream discussions. This systematic review synthesizes evidence from 167 peer-reviewed studies published between 2000 and 2026 to examine how pharmaceutical manufacturing effluents and clinical waste drive the dissemination of antibiotic resistance genes (ARGs) and multidrug-resistant bacteria in aquatic ecosystems. The literature was systematically identified and selected from major scientific databases using predefined inclusion and exclusion criteria. The reviewed evidence consistently shows that aquatic environments receiving inadequately treated pharmaceutical and healthcare waste which creates environmental hotspots characterized by elevated antibiotic residues, mobile genetic elements, and resistant microbial communities. Sub-inhibitory antibiotic concentrations, biofilm-mediated horizontal gene transfer and quorum-sensing dynamics collectively accelerate resistance gene dissemination beyond what direct selective killing alone would predict. Heavy metals, including copper, zinc, mercury, cadmium, and arsenic, further reinforce resistance through co-selection mechanisms, sustaining clinically important determinants such as blaNDM-1, blaKPC, and mcr-1 even in environments with limited antibiotic exposure. The review also highlights the role of non-pathogenic environmental bacteria as genetic reservoirs that facilitate the movement of resistance traits into pathogenic microorganisms. Metagenomic evidence confirms bidirectional exchange of resistance determinants between environmental and clinical bacterial populations, firmly situating the environmental resistome within a One Health framework. Despite growing evidence, important knowledge gaps remain regarding environmental-to-clinical transmission pathways, long-term ecological impacts, and the effectiveness of current mitigation strategies under diverse environmental conditions. Future research should prioritize longitudinal surveillance, multi-omics and systems biology approaches, metagenomic resistome profiling, and machine-learning-based predictive frameworks to better understand resistance dynamics across interconnected ecosystems. The findings support the integration of ARG monitoring into environmental management programs and underscore the need for advanced wastewater treatment technologies, harmonized discharge regulations, and coordinated One Health policies to curb the environmental amplification of antimicrobial resistance. These insights also contribute directly to achieving multiple Sustainable Development Goals, particularly SDG 1, 2, 3, 6, 9, 12, and 17 by fostering sustainable and integrated approaches to AMR mitigation. CI - Copyright (c) 2026 Tyagi, Rajput and Mishra. FAU - Tyagi, Anshnu AU - Tyagi A AD - School of Forensic Sciences, National Forensic Sciences University, Delhi, India. FAU - Rajput, Minakshi AU - Rajput M AD - Faculty of Forensic Science, Department of Chemistry, School of Physical Sciences, DIT University, Dehradun, Uttarakhand, India. FAU - Mishra, Sudhanshu AU - Mishra S AD - Faculty of Forensic Science, Department of Chemistry, School of Physical Sciences, DIT University, Dehradun, Uttarakhand, India. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Syst Biol JT - Frontiers in systems biology JID - 9918435075106676 PMC - PMC13714260 OTO - NOTNLM OT - One Health OT - antibiotic resistance genes OT - antimicrobial resistance OT - aquatic ecosystem OT - pharmaceutical and clinical waste OT - sustainable development goals COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:27 PHST- 2026/05/25 00:00 [received] PHST- 2026/07/14 00:00 [revised] PHST- 2026/07/16 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:27 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1890380 [pii] AID - 10.3389/fsysb.2026.1890380 [doi] PST - epublish SO - Front Syst Biol. 2026 Oct 14;6:1890380. doi: 10.3389/fsysb.2026.1890380. eCollection 2026. PMID- 42952979 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-875X (Print) IS - 2296-875X (Electronic) IS - 2296-875X (Linking) VI - 13 DP - 2026 TI - Factors influencing future liver remnant hypertrophy after portal vein embolization: a mini review. PG - 1979361 LID - 10.3389/fsurg.2026.1979361 [doi] LID - 1979361 AB - BACKGROUND: Portal vein embolization (PVE) promotes future liver remnant (FLR) hypertrophy before major hepatectomy, but the response varies substantially among patients. This review summarizes determinants of post-PVE FLR hypertrophy to support preoperative risk stratification and individualized planning. METHODS: Targeted searches of PubMed/MEDLINE, Web of Science, Scopus, Embase, and China National Knowledge Infrastructure (CNKI) through 26 August 2026 combined terms for PVE, liver regeneration or FLR hypertrophy, and predictors or associated factors. Reference lists and the authors' existing reference collection were also screened, and evidence was narratively synthesized across five domains. RESULTS: FLR hypertrophy reflects multiple interacting determinants. Advanced age, selected chemotherapy exposures, and liver fibrosis have been associated with weaker regenerative responses. Elevated inflammatory markers and biliary infection may impair hypertrophy, whereas adequate embolization extent and appropriate embolic material selection may improve the response. CONCLUSION: FLR hypertrophy after PVE is determined by interacting demographic and clinical, laboratory, hepatic and biliary, tumor-related, and procedure-related factors. Recognizing these determinants may improve patient selection, help predict insufficient hypertrophy, and guide individualized PVE strategies to increase the safety of major hepatectomy. CI - (c) 2026 Lu, Liu, Wu, Xiang, Zeng, Guo, Lu, Yang, Wang and Dong. FAU - Lu, Yuxuan AU - Lu Y AD - Hepato-Pancreato-Biliary Center, Beijing Tsinghua Changgung Hospital, Key Laboratory of Digital Intelligence Hepatology (Ministry of Education), School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. AD - Research Unit of Precision Hepatobiliary Surgery Paradigm, Chinese Academy of Medical Sciences, Beijing, China. FAU - Liu, Hangcheng AU - Liu H AD - Hepato-Pancreato-Biliary Center, Beijing Tsinghua Changgung Hospital, Key Laboratory of Digital Intelligence Hepatology (Ministry of Education), School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. AD - Research Unit of Precision Hepatobiliary Surgery Paradigm, Chinese Academy of Medical Sciences, Beijing, China. FAU - Wu, Boyang AU - Wu B AD - Hepato-Pancreato-Biliary Center, Beijing Tsinghua Changgung Hospital, Key Laboratory of Digital Intelligence Hepatology (Ministry of Education), School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. AD - Research Unit of Precision Hepatobiliary Surgery Paradigm, Chinese Academy of Medical Sciences, Beijing, China. FAU - Xiang, Canhong AU - Xiang C AD - Hepato-Pancreato-Biliary Center, Beijing Tsinghua Changgung Hospital, Key Laboratory of Digital Intelligence Hepatology (Ministry of Education), School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. AD - Research Unit of Precision Hepatobiliary Surgery Paradigm, Chinese Academy of Medical Sciences, Beijing, China. FAU - Zeng, Jianping AU - Zeng J AD - Hepato-Pancreato-Biliary Center, Beijing Tsinghua Changgung Hospital, Key Laboratory of Digital Intelligence Hepatology (Ministry of Education), School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. AD - Research Unit of Precision Hepatobiliary Surgery Paradigm, Chinese Academy of Medical Sciences, Beijing, China. FAU - Guo, Quanyu AU - Guo Q AD - Department of General Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. FAU - Lu, Hongwei AU - Lu H AD - Department of General Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China. FAU - Yang, Shizhong AU - Yang S AD - Hepato-Pancreato-Biliary Center, Beijing Tsinghua Changgung Hospital, Key Laboratory of Digital Intelligence Hepatology (Ministry of Education), School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. AD - Research Unit of Precision Hepatobiliary Surgery Paradigm, Chinese Academy of Medical Sciences, Beijing, China. FAU - Wang, Jitao AU - Wang J AD - Hepato-Pancreato-Biliary Center, Beijing Tsinghua Changgung Hospital, Key Laboratory of Digital Intelligence Hepatology (Ministry of Education), School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. AD - Research Unit of Precision Hepatobiliary Surgery Paradigm, Chinese Academy of Medical Sciences, Beijing, China. FAU - Dong, Jiahong AU - Dong J AD - Hepato-Pancreato-Biliary Center, Beijing Tsinghua Changgung Hospital, Key Laboratory of Digital Intelligence Hepatology (Ministry of Education), School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. AD - Research Unit of Precision Hepatobiliary Surgery Paradigm, Chinese Academy of Medical Sciences, Beijing, China. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Surg JT - Frontiers in surgery JID - 101645127 PMC - PMC13714361 OTO - NOTNLM OT - future liver remnant OT - liver hypertrophy OT - liver regeneration OT - liver venous deprivation OT - major hepatectomy OT - perihilar cholangiocarcinoma OT - portal vein embolization OT - posthepatectomy liver failure COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:27 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/11 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:27 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fsurg.2026.1979361 [doi] PST - epublish SO - Front Surg. 2026 Oct 14;13:1979361. doi: 10.3389/fsurg.2026.1979361. eCollection 2026. PMID- 42952978 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2673-6101 (Electronic) IS - 2673-6101 (Print) IS - 2673-6101 (Linking) VI - 7 DP - 2026 TI - Clinical observations during adjunctive Cannabis treatment and subsequent oclacitinib dose reduction in dogs with atopic dermatitis. PG - 1852631 LID - 10.3389/falgy.2026.1852631 [doi] LID - 1852631 AB - Canine atopic dermatitis is a multifactorial chronic skin disease that is difficult to control and requires lifelong and multimodal therapy. Cannabis has regained medical relevance in several fields, particularly in dermatology, as the endocannabinoid system is uniquely expressed in skin tissues and cells. Modulation through cannabinoids aims to restore skin homeostasis by reducing oxidative stress, inflammation, and consequently the clinical signs exhibited by animals with dermatopathies. This study aimed to evaluate Cannabis oil as a therapeutic option for dogs with atopic dermatitis, for both managing recurrent clinical signs and for enabling oclacitinib dose reduction. Sixteen dogs with atopic dermatitis previously treated with oclacitinib without satisfactory improvement were selected. All dogs underwent a fixed-sequence repeated-measures protocol consisting of four standardized evaluation stages: T0 (oclacitinib alone), T30 (placebo + oclacitinib), T60 (Cannabis oil + oclacitinib), and T90 (Cannabis oil + oclacitinib at 50% reduced dose). Dermatological assessments were performed at each stage to monitor clinical progression and response, including CADESI-4, performed by a veterinary dermatologist, and pruritus scoring, reported by the owners; both were blinded to whether the animal was receiving placebo (mineral oil) or Cannabis oil. Of the 16 enrolled dogs, fourteen completed all study stages through T90. No statistically significant difference was observed for CADESI-4. Likewise, no significant difference in pruritus was observed between T30 (placebo) and T60 (Cannabis oil); however, a significant reduction in pruritus was found between T0 x T60 (p = 0.0010), and T0 x T90 (p = 0.0025). Mild, transient, and self-limiting adverse events, as reported by the owners, were observed during both the placebo and Cannabis oil treatment periods. It is concluded that adjunctive Cannabis oil contributed to improved pruritus control in dogs with atopic dermatitis. In addition, maintenance of pruritus scores was observed following a predefined 50% reduction in oclacitinib dosage, supporting further investigation of Cannabis oil as part of a multimodal therapeutic strategy for canine atopic dermatitis. CI - (c) 2026 Mariga, Silva, Schiefler, Amazonas, Mateus, Schmidt and Filho. FAU - Mariga, Carollina AU - Mariga C AD - Postgraduate Program in Veterinary Medicine, Federal University of Santa Maria (PPGMV-UFSM), Santa Maria, Rio Grande do Sul, Brazil. FAU - da Silva, Filipe Costa AU - da Silva FC AD - Postgraduate Program in Veterinary Medicine, Federal University of Santa Maria (PPGMV-UFSM), Santa Maria, Rio Grande do Sul, Brazil. FAU - Schiefler, Otavio Henrique de Melo AU - Schiefler OHM AD - Postgraduate Program in Veterinary Medicine, Federal University of Santa Maria (PPGMV-UFSM), Santa Maria, Rio Grande do Sul, Brazil. FAU - Amazonas, Erik AU - Amazonas E AD - Postgraduate Program in Conventional and Integrative Veterinary Medicine (PPGMVCI), Federal University of Santa Catarina (UFSC), Curitibanos, Santa Catarina, Brazil. FAU - Mateus, Ana Lucia Souza Silva AU - Mateus ALSS AD - Department of Statistics, Federal University of Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil. FAU - Schmidt, Claudete AU - Schmidt C AD - Department of Small Animal Medicine, Federal University of Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil. FAU - Filho, Saulo Tadeu Lemos Pinto AU - Filho STLP AD - Postgraduate Program in Veterinary Medicine, Federal University of Santa Maria (PPGMV-UFSM), Santa Maria, Rio Grande do Sul, Brazil. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Allergy JT - Frontiers in allergy JID - 9918227355906676 PMC - PMC13713675 OTO - NOTNLM OT - canine atopic dermatitis OT - cannabidiol OT - endocannabinoid system OT - multimodal therapy OT - pruritus COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:27 PHST- 2026/04/14 00:00 [received] PHST- 2026/09/08 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:27 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/falgy.2026.1852631 [doi] PST - epublish SO - Front Allergy. 2026 Oct 14;7:1852631. doi: 10.3389/falgy.2026.1852631. eCollection 2026. PMID- 42952974 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-634X (Print) IS - 2296-634X (Electronic) IS - 2296-634X (Linking) VI - 14 DP - 2026 TI - Methylation-associated downregulation of CRABP1 marks an aggressive malignant epithelial cell state in thyroid carcinoma. PG - 1974822 LID - 10.3389/fcell.2026.1974822 [doi] LID - 1974822 AB - Papillary thyroid carcinoma (PTC) is the most common thyroid malignancy, yet molecular features that distinguish indolent tumors from those with progression-associated clinicopathological features remain limited. Here, we integrated single-cell RNA sequencing of 17 PTC samples with copy-number inference and identified a CRABP1-positive malignant epithelial cell population that retained epithelial features (CDH1, KRT19) and exhibited a distinct transcriptional program. In TCGA-THCA bulk data, lower CRABP1 expression was associated with lymph-node metastasis and pathological T-category variation. Functional experiments in complementary thyroid carcinoma cell-line models showed that CRABP1 knockdown increased migration and invasion and was accompanied by an EMT-related transcriptional shift, whereas CRABP1 overexpression showed the opposite pattern. Public methylation analyses showed higher aggregate promoter-region methylation in tumors, whereas methylation of cg02276974, an intron-2/gene-body CpG, was weakly inversely associated with CRABP1 expression (r = -0.2069, R(2) = 0.0428; P < 0.001). Decitabine altered migration, invasion, Vimentin-associated, and xenograft phenotypes in CRABP1-manipulated models. Collectively, these results identify a CRABP1-positive malignant epithelial cell state associated with clinicopathological features in PTC and support an invasion-associated suppressive role for CRABP1 in thyroid carcinoma models. The methylation and decitabine findings define a methylation-associated pharmacological context; they do not establish CRABP1-specific or locus-specific epigenetic causality. CI - Copyright (c) 2026 Fu, Li, Huang, Hu, Luo, Wang, Wang, Ding, Zhou and Li. FAU - Fu, Tiancheng AU - Fu T AD - Department of Thyroid Surgery, The Tenth Affiliated Hospital, Southern Medical University (Dongguan People's Hospital), Dongguan, Guangdong, China. FAU - Li, Yudong AU - Li Y AD - Department of Thyroid Surgery, The Tenth Affiliated Hospital, Southern Medical University (Dongguan People's Hospital), Dongguan, Guangdong, China. FAU - Huang, Guoxiang AU - Huang G AD - Department of Thyroid Surgery, The Tenth Affiliated Hospital, Southern Medical University (Dongguan People's Hospital), Dongguan, Guangdong, China. FAU - Hu, Xiarong AU - Hu X AD - Department of Thyroid Surgery, The Tenth Affiliated Hospital, Southern Medical University (Dongguan People's Hospital), Dongguan, Guangdong, China. FAU - Luo, Guangfeng AU - Luo G AD - Department of Thyroid Surgery, The Tenth Affiliated Hospital, Southern Medical University (Dongguan People's Hospital), Dongguan, Guangdong, China. FAU - Wang, Chengcheng AU - Wang C AD - Northern Jiangsu People's Hospital Affiliated to Yangzhou University, Yangzhou, Jiangsu, China. FAU - Wang, Yanze AU - Wang Y AD - The Fourth General Surgery Department of the Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning, China. FAU - Ding, Ziyang AU - Ding Z AD - Liaoning Fangda General Hospital, Shenyang, Liaoning, China. FAU - Zhou, Jinghua AU - Zhou J AD - Department of Thyroid Surgery, The Tenth Affiliated Hospital, Southern Medical University (Dongguan People's Hospital), Dongguan, Guangdong, China. FAU - Li, Zhen AU - Li Z AD - Department of Thyroid Surgery, The Tenth Affiliated Hospital, Southern Medical University (Dongguan People's Hospital), Dongguan, Guangdong, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Cell Dev Biol JT - Frontiers in cell and developmental biology JID - 101630250 PMC - PMC13713623 OTO - NOTNLM OT - CRABP1 OT - DNA methylation OT - decitabine OT - epithelial-mesenchymal transition OT - papillary thyroid carcinoma OT - single-cell RNA sequencing OT - thyroid carcinoma COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:27 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:27 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1974822 [pii] AID - 10.3389/fcell.2026.1974822 [doi] PST - epublish SO - Front Cell Dev Biol. 2026 Oct 14;14:1974822. doi: 10.3389/fcell.2026.1974822. eCollection 2026. PMID- 42952970 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-1078 (Print) IS - 1664-1078 (Electronic) IS - 1664-1078 (Linking) VI - 17 DP - 2026 TI - Comprehensive artificial intelligence-assisted mental health program for university students in China (CAMPUS): study protocol for a multi-center prospective cohort with planned embedded randomized intervention trials. PG - 1877887 LID - 10.3389/fpsyg.2026.1877887 [doi] LID - 1877887 AB - University students face a rising prevalence of depression, anxiety, insomnia, and suicidal ideation that exceeds the capacity of conventional, resource-constrained campus support services. Although artificial intelligence (AI)-assisted screening tools have emerged, many have evaluated selected modalities or isolated prediction tasks, and their integration with prospectively evaluated intervention pathways remains limited. This protocol describes the Comprehensive Artificial Intelligence-Assisted Mental Health Program for University Students in China (CAMPUS), a multi-center research program comprising a five-year prospective cohort, eight planned embedded randomized controlled trials, and exploratory multimodal AI and multi-omics studies across seven Chinese universities. Longitudinal cohort enrolment commenced at Henan Medical University in September 2025 and is planned to expand across seven participating universities. Multidimensional screening integrates psychological self-report (PHQ-9, GAD-7, ISI, BSI-CV, and others), physiological measurements (EEG, MRI, fNIRS, HRV, eye tracking, facial-expression analysis, ERP, voice acoustics), and an exploratory biospecimen component for multi-omics profiling, potentially including genomic, transcriptomic, proteomic, metabolomic, and microbiome analyses, subject to the finalization and approval of layer-specific protocols. Students identified through cohort screening as potentially eligible for an intervention trial will undergo trial-specific eligibility assessment. Those who meet the eligibility criteria and provide trial-specific informed consent will be individually randomized in a 1:1 ratio to the intervention or its comparator within one of eight separate two-arm, multi-center, parallel-group randomized controlled trials. A Transformer-based multimodal AI framework with SHAP-based feature attribution will be developed and internally evaluated for questionnaire-defined risk stratification and exploratory treatment-response prediction. The initial Henan Medical University cohort recruitment and university-student mental-health assessment were approved by the Ethics Review Committee of Henan Medical University (XYLL-20250484), and written informed consent is obtained from participants before enrolment. Each additional participating center will obtain local ethics approval before study activation. Findings will be disseminated through peer-reviewed publications, scientific conferences, and policy briefs, providing evidence that may inform the design and prospective evaluation of youth mental-health programs in other university settings. CLINICAL TRIAL REGISTRATION: The two clinical trials were registered in the Chinese Clinical Trial Registry (ChiCTR). The registration details and URLs are as follows: ChiCTR2500113527: https://www.chictr.org.cn/showproj.html?proj=298271; ChiCTR2500113613: https://www.chictr.org.cn/showproj.html?proj=298268. CI - Copyright (c) 2026 Yang, Liu, Wang, Zhu, Qin, Yan, Wang, Pei, Liang, Su, Wang, Wu, Ji, Wang and Wei. FAU - Yang, Guang AU - Yang G AD - Department of Early Intervention, Mental Health and Artificial Intelligence Research Center, The Second Affiliated Hospital of Henan Medical University, Henan Mental Hospital, Xinxiang, China. AD - School of Public Health, Henan Medical University, Xinxiang, China. AD - International Collaborative Laboratory for Air Pollution Health Effects and Intervention, Henan Medical University, Xinxiang, China. FAU - Liu, Rongxun AU - Liu R AD - Department of Early Intervention, Mental Health and Artificial Intelligence Research Center, The Second Affiliated Hospital of Henan Medical University, Henan Mental Hospital, Xinxiang, China. AD - School of Public Health, Henan Medical University, Xinxiang, China. FAU - Wang, Chuansheng AU - Wang C AD - Department of Early Intervention, Mental Health and Artificial Intelligence Research Center, The Second Affiliated Hospital of Henan Medical University, Henan Mental Hospital, Xinxiang, China. FAU - Zhu, Rongxin AU - Zhu R AD - Early Intervention Unit, Department of Psychiatry, Nanjing Brain Hospital, Nanjing Medical University, Nanjing, China. FAU - Qin, Shisen AU - Qin S AD - Department of Early Intervention, Mental Health and Artificial Intelligence Research Center, The Second Affiliated Hospital of Henan Medical University, Henan Mental Hospital, Xinxiang, China. FAU - Yan, Jinnan AU - Yan J AD - Department of Early Intervention, Mental Health and Artificial Intelligence Research Center, The Second Affiliated Hospital of Henan Medical University, Henan Mental Hospital, Xinxiang, China. FAU - Wang, Yan AU - Wang Y AD - Center for Psychological Development, China Medical University, Shenyang, China. FAU - Pei, Hongbo AU - Pei H AD - School of Public Health, Lanzhou University, Lanzhou, China. FAU - Liang, Lijuan AU - Liang L AD - Department of Psychology, The First Affiliated Hospital of Hainan Medical University, Haikou, China. FAU - Su, Yingying AU - Su Y AD - School of Medicine, Southern University of Science and Technology, Shenzhen, China. FAU - Wang, Shuai AU - Wang S AD - School of Psychology, Shandong Second Medical University, Weifang, China. FAU - Wu, Weidong AU - Wu W AD - School of Public Health, Henan Medical University, Xinxiang, China. AD - International Collaborative Laboratory for Air Pollution Health Effects and Intervention, Henan Medical University, Xinxiang, China. FAU - Ji, Guangjun AU - Ji G AD - Department of Early Intervention, Mental Health and Artificial Intelligence Research Center, The Second Affiliated Hospital of Henan Medical University, Henan Mental Hospital, Xinxiang, China. FAU - Wang, Fei AU - Wang F AD - Early Intervention Unit, Department of Psychiatry, Nanjing Brain Hospital, Nanjing Medical University, Nanjing, China. AD - Department of Mental Health, School of Public Health, Nanjing Medical University, Nanjing, China. AD - Department of Psychiatry, Yale School of Medicine, New Haven, CT, United States. FAU - Wei, Yange AU - Wei Y AD - Department of Early Intervention, Mental Health and Artificial Intelligence Research Center, The Second Affiliated Hospital of Henan Medical University, Henan Mental Hospital, Xinxiang, China. AD - Department of Psychiatry, Peking University Sixth Hospital, Peking University Institute of Mental Health, NHC Key Laboratory of Mental Health (Peking University), National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Beijing, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Psychol JT - Frontiers in psychology JID - 101550902 PMC - PMC13713620 OTO - NOTNLM OT - artificial intelligence OT - digital mental health platform OT - individualized psychological intervention OT - multi-center cohort study OT - multi-omics OT - multidimensional screening OT - study protocol OT - university students COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:27 PHST- 2026/05/11 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:27 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpsyg.2026.1877887 [doi] PST - epublish SO - Front Psychol. 2026 Oct 14;17:1877887. doi: 10.3389/fpsyg.2026.1877887. eCollection 2026. PMID- 42952948 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-0640 (Print) IS - 1664-0640 (Electronic) IS - 1664-0640 (Linking) VI - 17 DP - 2026 TI - Longitudinal relationships between anxiety and fear of progression in post-CABG patients: a cross-sectional and cross-lagged panel network analysis. PG - 1937200 LID - 10.3389/fpsyt.2026.1937200 [doi] LID - 1937200 AB - BACKGROUND: Patients following coronary artery bypass grafting (CABG) frequently experience elevated anxiety and fear of progression (FoP), which has a high comorbidity and significantly increases adverse postoperative event risks. This prospective study explored the dynamic longitudinal associations between anxiety and FoP in post-CABG patients using network analysis methods. METHODS: A prospective longitudinal design enrolled first-time CABG patients from three tertiary hospitals in Urumqi, China. Anxiety and FoP were assessed using the Generalized Anxiety Disorder-7 (GAD-7) and the Fear of Progression Questionnaire-Short Form (FoP-Q-SF) at three time points: hospital discharge (T1), 1 month (T2), and 3 months (T3) post-discharge. Cross-sectional Gaussian Graphical Models were constructed at each time point, and cross-lagged panel network (CLPN) analysis was performed to examine longitudinal symptom associations. Six a priori selected covariates (age, gender, educational level, duration of CHD, chronic comorbidities, and NYHA functional classification) were residualized prior to network estimation. RESULTS: A total of 343 patients were included in the network analysis. From T1 to T3, total GAD-7 scores demonstrated a notable downward trend, whereas FoP-Q-SF scores exhibited a continuous upward trend. The core node at both T1 and T2 was FoP11 "Worrying about what will become of the family if something should happen to the patient, " which shifted to FoP2 "Being nervous prior to doctors' appointments or periodic examinations" at T3. In the T1-T2 CLPN, the strongest cross-lagged edge was from GAD7 "Feeling afraid as if something awful might happen" to FoP2 "Being nervous prior to doctors' appointments or periodic examinations". In the T2-T3 CLPN, the strongest cross-lagged edge was from GAD2 "Not being able to stop or control worrying" to FoP6 "Being afraid of the possibility that the children could contract disease". In the T1-T3 network, the strongest cross-lagged edge was from GAD4 "Trouble relaxing" to FoP3 "Being afraid of pain". Anxiety nodes generally exhibited higher Out-Expected Influence, while FoP nodes showed higher In-Expected Influence. CONCLUSION: Anxiety levels gradually decreased during early rehabilitation, whereas fear of progression increased over time. Early anxiety symptoms showed positive cross-lagged associations with later fear of progression. These findings suggest that clinical nurses should consider phased, precision-based psychological interventions targeting stage-specific symptoms during different rehabilitation stages. CI - Copyright (c) 2026 Luo, Chen, Li, Wang, Chen, Guo, Xing, Liang and Tao. FAU - Luo, Zhengwei AU - Luo Z AD - Department of Nursing, Xinjiang Medical University, Urumqi, China. FAU - Chen, Fenghui AU - Chen F AD - Department of Nursing, Xinjiang Medical University, Urumqi, China. FAU - Li, Huihui AU - Li H AD - Department of Nursing, Xinjiang Medical University, Urumqi, China. FAU - Wang, Weilong AU - Wang W AD - Department of Nursing, Xinjiang Medical University, Urumqi, China. FAU - Chen, Duohuan AU - Chen D AD - Department of Nursing, Xinjiang Medical University, Urumqi, China. FAU - Guo, Yongzhong AU - Guo Y AD - Department of Cardiac Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China. FAU - Xing, Juan AU - Xing J AD - Department of Cardiac Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi, China. FAU - Liang, Fang AU - Liang F AD - Department of Nursing, Xinjiang Medical University, Urumqi, China. FAU - Tao, Jianshuang AU - Tao J AD - Department of Cardiac Surgery, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Psychiatry JT - Frontiers in psychiatry JID - 101545006 PMC - PMC13713660 OTO - NOTNLM OT - anxiety OT - coronary artery bypass grafting OT - cross-lagged panel network analysis OT - fear of progression OT - network analysis COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:25 PHST- 2026/07/14 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:25 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpsyt.2026.1937200 [doi] PST - epublish SO - Front Psychiatry. 2026 Oct 14;17:1937200. doi: 10.3389/fpsyt.2026.1937200. eCollection 2026. PMID- 42952947 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2688-4844 (Electronic) IS - 2688-4844 (Linking) VI - 7 DP - 2026 Jan-Dec TI - Multimodal Hysterosalpingography for One-Stop Assessment of Tubal Patency and Fimbrial Function: A Prospective Diagnostic Trial. PG - 26884844261434409 LID - 10.1177/26884844261434409 [doi] LID - 26884844261434409 AB - BACKGROUND: Tubal infertility is a major cause of female infertility, necessitating accurate evaluation of fallopian tube patency and fimbrial function. While a laparoscopic dye test (LDT) remains the gold standard, its invasiveness limits clinical use. This study proposes a multimodal hysterosalpingography approach combining four-dimensional (4D) contrast-enhanced ultrasound (4D HyCoSy), two-dimensional color Doppler imaging (2D-CIS), and 2D baseline scanning with saline-infused uterine cavity contrast (2D-FS + SIPS) for noninvasive, comprehensive tubal assessment. METHODS: A prospective study included 136 infertile patients (242 fallopian tubes) from March 2022 to August 2025. All patients underwent multimodal hysterosalpingography. Diagnostic performance was compared against LDT, and correlations between fimbrial morphology and tubal function were analyzed. RESULTS: Multimodal integration significantly improved diagnostic accuracy: sensitivity for patency, partial obstruction, and total obstruction was 93.94%, 85.29%, and 90.7%, respectively, with a specificity of 97.49%, outperforming single-modality examinations (p < 0.05). The addition of 2D-FS + SIPS enhanced visualization of fimbrial structures, increasing detection rates from 34.7% to 62.1% (p < 0.001). Abnormal fimbrial morphology strongly correlated with tubal dysfunction, with abnormality rates rising from 16.9% (patent) to 61.1% (partially obstructed) and 95.1% (obstructed) (p < 0.001). Multimodal evaluation showed 88.6% concordance with LDT (kappa = 0.765), sensitivity of 92.9%, and specificity of 94.4%. CONCLUSION: Multimodal hysterosalpingography provides a noninvasive, one-stop solution for precise evaluation of tubal patency and fimbrial function. It identifies fimbrial pathology as a key factor in distal tubal obstruction, offering valuable insights for etiology diagnosis and personalized treatment of tubal infertility. CI - (c) The Author(s). FAU - Xie, Lijun AU - Xie L AD - Department of Ultrasound Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China. AD - Department of Ultrasound Medicine, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, China. FAU - Huang, Liyan AU - Huang L AD - Department of Ultrasound Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China. AD - Department of Ultrasound Medicine, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, China. FAU - Li, Haiying AU - Li H AD - Department of Ultrasound Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China. AD - Department of Ultrasound Medicine, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, China. FAU - Wei, Weili AU - Wei W AD - Department of Ultrasound Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China. AD - Department of Ultrasound Medicine, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, China. FAU - Zhou, Ting AU - Zhou T AD - Department of Ultrasound Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China. AD - Department of Ultrasound Medicine, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, China. FAU - Gan, Ling AU - Gan L AD - Department of Ultrasound Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China. AD - Department of Ultrasound Medicine, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, China. FAU - Liu, Xinxiu AU - Liu X AD - Department of Ultrasound Medicine, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China. AD - Department of Ultrasound Medicine, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, China. LA - eng PT - Journal Article DEP - 20260403 PL - United States TA - Womens Health Rep (New Rochelle) JT - Women's health reports (New Rochelle, N.Y.) JID - 101768931 PMC - PMC13714191 OTO - NOTNLM OT - 4D-HyCoSy OT - fimbrial function OT - multimodal hysterosalpingography OT - noninvasive diagnosis OT - tubal patency COIS- No competing financial interests exist. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/04/03 CRDT- 2026/10/29 05:25 PHST- 2026/03/03 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:25 [entrez] PHST- 2026/04/03 00:00 [pmc-release] AID - 10.1177_26884844261434409 [pii] AID - 10.1177/26884844261434409 [doi] PST - epublish SO - Womens Health Rep (New Rochelle). 2026 Apr 3;7:26884844261434409. doi: 10.1177/26884844261434409. eCollection 2026 Jan-Dec. PMID- 42952945 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 2731-717X (Electronic) IS - 2731-7161 (Print) IS - 2731-7161 (Linking) VI - 71 IP - 6 DP - 2026 TI - [Internet-based self-help for loneliness from the users' perspective]. PG - 452-460 LID - 10.1007/s00278-026-00881-x [doi] AB - Loneliness is associated with a range of adverse health outcomes. Internet-based self-help interventions offer a low-threshold approach to reducing loneliness; however, little is known about users' experiences. The aim of this study was to qualitatively examine users' experiences with the internet-based self-help intervention SOLUS‑D for the reduction of loneliness. The data were derived from a randomized controlled trial evaluating SOLUS‑D. Following the 10-week intervention period, semistructured telephone interviews were conducted with the participants. For the present analysis, 17 interviews were selected and analyzed using reflexive thematic analysis. Three main themes were identified. 1: participants reported changes in thinking, feeling and behavior, including increased awareness of automatic thoughts, greater clarity in dealing with feelings of loneliness, behavioral activation and enhanced self-efficacy. 2: the role of guidance emerged as important: automated messages were primarily perceived as reminders, whereas human guidance fostered motivation, a sense of safety and the feeling of being seen. 3: the findings indicated that SOLUS‑D was not equally suitable for all users. In particular, participants seeking direct social interaction or concrete opportunities to form relationships perceived limitations of the self-help format. The results suggest that internet-based self-help interventions can represent a relevant low-threshold option for addressing loneliness. They also highlight the importance for greater personalization of content and guidance specifically addressing individual and social factors that influence loneliness. CI - (c) The Author(s) 2026. FAU - Seewer, Noemi AU - Seewer N AUID- ORCID: 0000-0002-1208-629X AD - Abteilung fur Klinische Psychologie und Psychotherapie, Institut fur Psychologie, Universitat Bern, Fabrikstrasse 8, 3012 Bern, Schweiz. ROR: https://ror.org/02k7v4d05. GRID: grid.5734.5. ISNI: 0000 0001 0726 5157 FAU - Krieger, Tobias AU - Krieger T AD - Abteilung fur Klinische Psychologie und Psychotherapie, Institut fur Psychologie, Universitat Bern, Fabrikstrasse 8, 3012 Bern, Schweiz. ROR: https://ror.org/02k7v4d05. GRID: grid.5734.5. ISNI: 0000 0001 0726 5157 LA - ger PT - English Abstract PT - Journal Article TT - Internetbasierte Selbsthilfe bei Einsamkeit aus Sicht der Nutzenden: Eine reflexive thematische Analyse. DEP - 20261006 PL - Germany TA - Psychotherapie (Heidelb) JT - Psychotherapie (Heidelberg, Germany) JID - 9918383865606676 PMC - PMC13714050 OTO - NOTNLM OT - Cognitive behavioral therapy OT - Guidance OT - Perceived social isolation OT - Psychological intervention OT - Qualitative analysis EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/06 CRDT- 2026/10/29 05:25 PHST- 2026/05/05 00:00 [received] PHST- 2026/09/07 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:25 [entrez] PHST- 2026/10/06 00:00 [pmc-release] AID - 881 [pii] AID - 10.1007/s00278-026-00881-x [doi] PST - ppublish SO - Psychotherapie (Heidelb). 2026;71(6):452-460. doi: 10.1007/s00278-026-00881-x. Epub 2026 Oct 6. PMID- 42952944 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Dexmedetomidine-containing patient-controlled intravenous analgesia after elective caesarean delivery: a retrospective cohort study. PG - 1991923 LID - 10.3389/fmed.2026.1991923 [doi] LID - 1991923 AB - BACKGROUND: Dexmedetomidine is used as an adjunct in multimodal postoperative analgesia, but evidence from routine clinical care after caesarean delivery remains limited. We evaluated whether dexmedetomidine-containing patient-controlled intravenous analgesia (PCIA) was associated with postoperative analgesic and early recovery outcomes. METHODS: This single-centre retrospective cohort study included women aged 18-45 years undergoing elective term caesarean delivery between January and September 2025. Women were classified according to the initial postoperative PCIA regimen as receiving dexmedetomidine-containing or conventional PCIA. The primary outcome was movement pain on a 0-10 numerical rating scale at 24 h. Secondary outcomes included pain through 48 h, sufentanil delivery, patient-controlled analgesia demands, rescue analgesia, treatment-related events, and early recovery milestones. Propensity-score overlap weighting was used for the primary analysis. Propensity-score matching, conventional multivariable adjustment, complete-case and restricted-cohort analyses, and an expanded propensity-score model incorporating attending anaesthesiologist and calendar month were used to assess robustness. RESULTS: Among 304 eligible women, 132 received dexmedetomidine-containing PCIA and 172 received conventional PCIA. Mean movement pain at 24 h was 3.1 (SD 1.2) versus 3.7 (SD 1.3), corresponding to an overlap-weighted mean difference of -0.51 points (95% CI -0.80 to -0.22; P < 0.001). The primary estimate was similar across sensitivity analyses, including the expanded propensity-score model incorporating attending anaesthesiologist and calendar month. Among 290 women with complete 48-h pump records, median cumulative sufentanil delivery was 84 mug (IQR 78-91) versus 90 mug (82-98; median difference -5.2 mug, 95% CI -7.8 to -2.6; P < 0.001). Rescue analgesia was required in 24 of 132 women (18.2%) versus 46 of 172 (26.7%; risk ratio 0.72, 95% CI 0.52-0.99; P = 0.044). First mobilisation occurred at 16.9 versus 19.3 h (time ratio 0.89, 95% CI 0.84-0.95; P < 0.001), and first passage of flatus at 25.8 versus 28.9 h (time ratio 0.92, 95% CI 0.86-0.98; P = 0.012). Estimates for uncommon adverse events were imprecise. CONCLUSIONS: In this retrospective cohort, dexmedetomidine-containing PCIA was associated with modestly lower early movement pain and lower analgesic requirements, with shorter observed times to selected early recovery milestones. Estimates for uncommon adverse events did not establish safety equivalence. These observational findings should not be interpreted as establishing a causal treatment effect. CI - (c) 2026 Lu, Huang, Hu, Ying, Quan, Li, Li and Cheng. FAU - Lu, YinJun AU - Lu Y AD - Department of Anesthesiology, Yongkang Women and Children's Health Hospital, Yongkang, Zhejiang, China. FAU - Huang, YuHui AU - Huang Y AD - Department of Anesthesiology, Yongkang Women and Children's Health Hospital, Yongkang, Zhejiang, China. FAU - Hu, ChaoRen AU - Hu C AD - Department of Anesthesiology, Yongkang Women and Children's Health Hospital, Yongkang, Zhejiang, China. FAU - Ying, Li AU - Ying L AD - Department of Anesthesiology, Yongkang Women and Children's Health Hospital, Yongkang, Zhejiang, China. FAU - Quan, ShuCan AU - Quan S AD - Department of Anesthesiology, Yongkang Women and Children's Health Hospital, Yongkang, Zhejiang, China. FAU - Li, Lei AU - Li L AD - Department of Laboratory Medicine, Yongkang Women and Children's Health Hospital, Yongkang, Zhejiang, China. FAU - Li, Xiongying AU - Li X AD - Department of Obstetrics, Yongkang Women and Children's Health Hospital, Yongkang, Zhejiang, China. FAU - Cheng, YingLi AU - Cheng Y AD - Department of Anesthesiology, Yongkang Women and Children's Health Hospital, Yongkang, Zhejiang, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13713642 OTO - NOTNLM OT - caesarean delivery OT - dexmedetomidine OT - multimodal analgesia OT - patient-controlled intravenous analgesia OT - postoperative recovery COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:24 PHST- 2026/09/08 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:24 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmed.2026.1991923 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 14;13:1991923. doi: 10.3389/fmed.2026.1991923. eCollection 2026. PMID- 42952940 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2297-055X (Print) IS - 2297-055X (Electronic) IS - 2297-055X (Linking) VI - 13 DP - 2026 TI - Developing a case management program and clinical practice for high-risk sudden cardiac death patients through the lens of family systems theory. PG - 1903575 LID - 10.3389/fcvm.2026.1903575 [doi] LID - 1903575 AB - OBJECTIVE: To develop a case management plan for patients at high risk of sudden cardiac death (SCD) from the perspective of family systems theory and evaluate its clinical practice effects. METHODS: By using the random sampling method, 100 patients at high risk of SCD who visited our hospital from March 2025 to June 2025 were selected as the research subjects. They were randomly divided into an experimental group (n = 50) and a control group (n = 50). The control group received routine care and follow-up, while the experimental group implemented a case management plan based on the family systems theory, with both interventions lasting for one year. Both groups were evaluated at baseline and endpoint using disease cognition assessments, the Patient Health Questionnaire-9 (PHQ-9), Generalized Anxiety Disorder-7 (GAD-7), Tampa Scale for Kinesiophobia Heart (TSK-SV Heart), General Self-Efficacy Scale (GSES), Family APGAR, and Major Adverse Cardiac Events (MACE). RESULTS: There were no statistically significant differences in baseline data between the two groups (P > 0.05). After the intervention, the experimental group showed significantly better scores in disease cognition, PHQ-9, GAD-7, TSK-SV Heart, GSES, APGAR, and MACE compared to the control group (P < 0.05). CONCLUSION: The case management plan for high-risk SCD patients based on family systems theory is scientifically sound and feasible. It can effectively reduce anxiety and depression, alleviate kinesiophobia, improve disease cognition, and promote family care, thereby improving the patients' quality of life and prognosis. CI - (c) 2026 Liu, Li, Zhang, Yang, Yuan, Jia, Wang, Luo, Li, Chen, Gao and Zhang. FAU - Liu, Fulan AU - Liu F AD - Nursing Department, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. FAU - Li, Caihong AU - Li C AD - Nursing Department, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. FAU - Zhang, Zhaoguo AU - Zhang Z AD - Nursing Department, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. FAU - Yang, Jing AU - Yang J AD - Nursing Department, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. FAU - Yuan, Ke AU - Yuan K AD - Nursing Department, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. FAU - Jia, Xuewei AU - Jia X AD - Nursing Department, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. FAU - Wang, Wen AU - Wang W AD - Nursing Department, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. FAU - Luo, Jiangying AU - Luo J AD - Nursing Department, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. FAU - Li, Dan AU - Li D AD - Nursing Department, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. FAU - Chen, Lingling AU - Chen L AD - Nursing Department, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. FAU - Gao, Fengli AU - Gao F AD - Nursing Department, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. FAU - Zhang, Ping AU - Zhang P AD - Nursing Department, Beijing Tsinghua Changgung Hospital, School of Clinical Medicine, Tsinghua Medicine, Tsinghua University, Beijing, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Cardiovasc Med JT - Frontiers in cardiovascular medicine JID - 101653388 PMC - PMC13713636 OTO - NOTNLM OT - case management OT - family care OT - family systems theory OT - quality of life OT - sudden cardiac death COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:24 PHST- 2026/06/08 00:00 [received] PHST- 2026/08/27 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:24 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fcvm.2026.1903575 [doi] PST - epublish SO - Front Cardiovasc Med. 2026 Oct 14;13:1903575. doi: 10.3389/fcvm.2026.1903575. eCollection 2026. PMID- 42952939 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2193-9616 (Print) IS - 2193-9616 (Electronic) IS - 2193-9616 (Linking) VI - 14 IP - 3 DP - 2026 TI - Ethnopharmacological evaluation of Irvingia gabonensis (Aubry-Lecomte ex O'Rorke) Baill. seed extract: integrated computational and in vitro assessment of antioxidant, antidiabetic, and anticancer activities with in vivo validation of antidiabetic activity. PG - 275 LID - 10.1007/s40203-026-00779-1 [doi] LID - 275 AB - Irvingia gabonensis has been extensively investigated for its phytochemical composition and diverse biological activities, including antioxidant and metabolic effects. However, integrated studies combining phytochemical characterization with computational assessment of multiple disease-relevant molecular targets and corresponding experimental biological endpoints remain comparatively limited. This study therefore evaluated the antioxidant, antidiabetic, and anticancer potential of the methanol seed extract of I. gabonensis using an integrated approach comprising phytochemical profiling, computational analyses, and in vitro biological assays, with in vivo validation specifically restricted to antidiabetic activity in streptozotocin-induced diabetic rats. Preliminary phytochemical screening and LC-MS/MS profiling revealed phenolics, flavonoids, tannins, phytosterols, and triterpenoids, including quercetin, kaempferol, chlorogenic acid, rutin, beta-sitosterol, oleanolic acid, and ursolic acid. The extract exhibited total phenolic and flavonoid contents of 185.6 +/- 4.2 mg GAE/g and 96.4 +/- 2.8 mg QE/g, respectively. Molecular docking indicated favorable docking scores and predicted interactions of selected phytochemicals with alpha-amylase, alpha-glucosidase, DPP-4, Keap1, BCL-2, EGFR, and VEGFR-2, with quercetin showing favorable predicted interactions across several targets. In vitro, the extract showed concentration-dependent antioxidant activity, with DPPH and ABTS IC(5)(0) values of 82.6 +/- 3.1 and 76.3 +/- 2.7 mug/mL, respectively, and inhibited alpha-amylase (IC(5)(0) = 112.4 +/- 4.6 mug/mL) and alpha-glucosidase (IC(5)(0) = 104.3 +/- 4.1 mug/mL). Anticancer activity was evaluated exclusively in vitro. The extract exhibited differential cytotoxicity against PC-3, MCF-7, and HCT116 cancer cells compared with the non-malignant HEK293 comparator cell line, with lower cytotoxicity toward HEK293 cells under the tested conditions. Annexin V/PI analysis further demonstrated increased apoptotic cell populations in extract-treated cancer cells. In vivo evaluation was restricted to antidiabetic activity. In streptozotocin-induced diabetic rats, treatment with the extract (400 mg/kg) reduced fasting blood glucose and HbA1c, restored insulin levels, improved lipid profiles, enhanced endogenous antioxidant defenses, reduced lipid peroxidation, and ameliorated diabetes-associated histopathological alterations. Collectively, these findings provide computational, in vitro, and in vivo evidence supporting the antidiabetic potential of I. gabonensis seed extract. The observed anticancer effects remain limited to in vitro evidence, and the differential cytotoxicity observed relative to HEK293 cells should not be interpreted as evidence of tumour selectivity. Further studies employing tissue-matched non-malignant cells, appropriate animal tumour models, and mechanistic, pharmacokinetic, and toxicological investigations are required to establish its anticancer potential. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at https://doi.org/10.1007/s40203-026-00779-1. CI - (c) The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2026. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. FAU - Ahuchaogu, Ahamefula Anslem AU - Ahuchaogu AA AD - Department of Pure and Industrial Chemistry, Abia State University, Uturu, Nigeria. ROR: https://ror.org/03a39z514. GRID: grid.442675.6. ISNI: 0000 0000 9756 5366 FAU - Ukwubile, Cletus Anes AU - Ukwubile CA AUID- ORCID: 0000-0001-7183-4510 AD - Department of Pharmacognosy, Faculty of Pharmacy, University of Maiduguri, Maiduguri, Nigeria. ROR: https://ror.org/016na8197. GRID: grid.413017.0. ISNI: 0000 0000 9001 9645 FAU - Nwabekee, Ifeoma Uzoamaka AU - Nwabekee IU AD - Department of Pure and Industrial Chemistry, Abia State University, Uturu, Nigeria. ROR: https://ror.org/03a39z514. GRID: grid.442675.6. ISNI: 0000 0000 9756 5366 FAU - Nwagba, James Onyedikachi AU - Nwagba JO AD - Department of Pure and Industrial Chemistry, Abia State University, Uturu, Nigeria. ROR: https://ror.org/03a39z514. GRID: grid.442675.6. ISNI: 0000 0000 9756 5366 LA - eng PT - Journal Article DEP - 20261027 PL - Germany TA - In Silico Pharmacol JT - In silico pharmacology JID - 101623954 PMC - PMC13713973 OTO - NOTNLM OT - Antioxidant activity OT - Apoptosis OT - Diabetes mellitus OT - Irvingia gabonensis OT - Molecular docking COIS- Conflict of interestsThe authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2027/10/27 CRDT- 2026/10/29 05:24 PHST- 2026/08/06 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:24 [entrez] PHST- 2027/10/27 00:00 [pmc-release] AID - 779 [pii] AID - 10.1007/s40203-026-00779-1 [doi] PST - epublish SO - In Silico Pharmacol. 2026 Oct 27;14(3):275. doi: 10.1007/s40203-026-00779-1. eCollection 2026. PMID- 42952935 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2168-8184 (Print) IS - 2168-8184 (Electronic) IS - 2168-8184 (Linking) VI - 18 IP - 9 DP - 2026 Sep TI - Emerging Biomarkers in Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD): Current Evidence, Clinical Utility, and Future Perspectives. PG - e117033 LID - 10.7759/cureus.117033 [doi] LID - e117033 AB - Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most prevalent chronic liver disease worldwide and represents the hepatic manifestation of metabolic syndrome. The disease spectrum ranges from simple steatosis to steatohepatitis, fibrosis, cirrhosis, and hepatocellular carcinoma. Liver biopsy remains the histological reference standard for assessing steatohepatitis and microscopic disease activity and is valuable in selected cases; however, contemporary MASLD management increasingly relies on validated non-invasive tests for fibrosis risk stratification, with biopsy generally reserved for diagnostic uncertainty, discordant non-invasive findings, or situations in which histological information would alter management. This review aims to summarize the biochemical basis, pathophysiological mechanisms, current evidence, clinical utility, and limitations of emerging non-invasive biomarkers for the diagnosis, staging, and longitudinal monitoring of MASLD. A comprehensive narrative review of recent advances in molecular biology, metabolomics, proteomics, and inflammatory pathway research was conducted, synthesizing evidence on promising circulating biomarkers and their role in reflecting hepatic steatosis, lipotoxicity, apoptosis, inflammation, and fibrogenesis. This narrative review was conducted through a structured literature search of PubMed/MEDLINE, Scopus, and Google Scholar. Search terms included combinations of 'metabolic dysfunction-associated steatotic liver disease,' 'MASLD,' 'MASH,' 'non-invasive biomarkers,' 'cytokeratin-18,' 'FGF-21,' 'adipokines,' 'cytokines,' 'extracellular vesicles,' 'microRNAs,' 'metabolomics,' 'gut microbiota,' 'fibrosis biomarkers,' and 'non-invasive liver tests.' Priority was given to human studies, systematic reviews, meta-analyses, multicenter validation studies, and major professional-society guidelines relevant to the diagnosis, fibrosis risk stratification, prognosis, and treatment-response monitoring of MASLD. Reference lists of relevant articles were additionally reviewed to identify pertinent studies. As this was a narrative rather than a systematic review, no formal meta-analysis or standardized risk-of-bias assessment was performed. Recent research has identified several promising biomarkers, including cytokeratin-18 fragments, fibroblast growth factor-21 (FGF-21), adipokines, inflammatory cytokines, extracellular vesicles, circulating microRNAs, metabolomic signatures, and gut microbiota-derived metabolites. These biomarkers have demonstrated potential not only for diagnosing MASLD and distinguishing steatohepatitis from simple steatosis but also for predicting disease progression and monitoring therapeutic responses. However, no single biomarker currently meets all criteria to universally replace liver biopsy. Integrated multi-marker panels, enhanced by genetic data and artificial intelligence-driven algorithms, represent the most pragmatic strategy moving forward, with the aspirational goal of achieving a non-invasive, blood-based "virtual liver biopsy" in routine clinical practice. CI - Copyright (c) 2026, Dhanavade et al. FAU - Dhanavade, Sushant S AU - Dhanavade SS AD - Biochemistry, Krishna Institute of Medical Sciences, Krishna Vishwa Vidyapeeth (Deemed to Be University), Karad, IND. FAU - Kshirsagar, Mandakini S AU - Kshirsagar MS AD - Biochemistry, Krishna Institute of Medical Sciences, Krishna Vishwa Vidyapeeth (Deemed to Be University), Karad, IND. FAU - Vani, Axita AU - Vani A AD - Biochemistry, Krishna Institute of Medical Sciences, Krishna Vishwa Vidyapeeth (Deemed to Be University), Karad, IND. FAU - Mujawar, Altaf K AU - Mujawar AK AD - Biochemistry, Krishna Institute of Medical Sciences, Krishna Vishwa Vidyapeeth (Deemed to Be University), Karad, IND. FAU - Bansode, Pankaj V AU - Bansode PV AD - Biochemistry, Krishna Institute of Medical Sciences, Krishna Vishwa Vidyapeeth (Deemed to Be University), Karad, IND. LA - eng PT - Journal Article PT - Review DEP - 20260927 PL - United States TA - Cureus JT - Cureus JID - 101596737 PMC - PMC13714188 OTO - NOTNLM OT - adipokines OT - biomarkers OT - cytokeratin-18 OT - fgf-21 OT - liver fibrosis OT - masld OT - metabolomics OT - nafld OT - steatohepatitis COIS- Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/27 CRDT- 2026/10/29 05:24 PHST- 2026/08/04 00:00 [received] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:24 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - 10.7759/cureus.117033 [doi] PST - epublish SO - Cureus. 2026 Sep 27;18(9):e117033. doi: 10.7759/cureus.117033. eCollection 2026 Sep. PMID- 42952930 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2235-2988 (Electronic) IS - 2235-2988 (Linking) VI - 16 DP - 2026 TI - Circulating calprotectin and S100A8/A9-related biomarkers for diagnosis and prognostic stratification in adult acute infection and sepsis: a systematic review and meta-analysis. PG - 1934020 LID - 10.3389/fcimb.2026.1934020 [doi] LID - 1934020 AB - BACKGROUND: Circulating calprotectin is a proposed marker of acute innate immune activation, but reported diagnostic and prognostic performance is inconsistent. We assessed circulating calprotectin and S100A8/A9-related biomarkers for diagnosing acute infection and sepsis and stratifying prognosis in adults. METHODS: We searched biomedical databases and trial registries through July 4, 2026, for adult studies of circulating calprotectin/S100A8/A9 in acute infection or sepsis. Random-effects models pooled diagnostic and reported prognostic areas under the receiver operating characteristic curve (AUC). Threshold-based sensitivity and specificity were analyzed separately using bivariate random-effects models. We used the Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2) and Grading of Recommendations Assessment, Development and Evaluation (GRADE) to assess diagnostic risk of bias and evidence certainty. Exploratory subgroup, sensitivity, biomarker-comparison, and combined-model analyses were also performed. RESULTS: Twenty-two reports representing 21 independent cohorts were included. The primary diagnostic meta-analysis yielded a pooled AUC of 0.741 (95% confidence interval [CI], 0.657-0.810). The pooled AUC was 0.703 (95% CI, 0.600-0.789) for sepsis and 0.758 (95% CI, 0.636-0.849) for acute infection or infection-source-related outcomes. In separate threshold-based analyses, pooled sensitivity and specificity were 0.70 (95% CI, 0.46-0.86) and 0.57 (95% CI, 0.55-0.59) for sepsis, and 0.46 (95% CI, 0.09-0.88) and 0.86 (95% CI, 0.60-0.96) for acute infection or infection-source-related outcomes, respectively. For mortality/survival outcomes, pooled sensitivity and specificity were 0.72 (95% CI, 0.65-0.77) and 0.66 (95% CI, 0.53-0.77), respectively, while the pooled reported prognostic AUC was 0.737 (95% CI, 0.688-0.786). Exploratory analyses were directionally stable, but paired biomarker comparisons did not provide definitive evidence of superiority. Certainty was very low for all major outcomes. CONCLUSIONS: Circulating calprotectin and S100A8/A9-related biomarkers provide moderate, context-dependent information in adult acute infection and sepsis. They may serve as adjunctive host-response markers, particularly for selected infection/source contrasts, but current evidence does not support stand-alone diagnosis, universal thresholds, or replacement of established biomarkers and clinical assessment. SYSTEMATIC REVIEW REGISTRATION: https://www.crd.york.ac.uk/PROSPERO/view/CRD420261441569, identifier CRD420261441569. CI - Copyright (c) 2026 Liao, Deng, Song, Yu, Li, Lu and Chen. FAU - Liao, Yin-Sheng AU - Liao YS AD - Department of Anesthesiology, Zigong Fourth People's Hospital, Zigong, Sichuan, China. FAU - Deng, Yu-Ping AU - Deng YP AD - Department of Anesthesiology, Zigong Hospital of Chengdu University of Traditional Chinese Medicine, Zigong, Sichuan, China. FAU - Song, Jian-Li AU - Song JL AD - Department of Anesthesiology, Zigong Fourth People's Hospital, Zigong, Sichuan, China. FAU - Yu, Xuan AU - Yu X AD - Department of Anesthesiology, Zigong Fourth People's Hospital, Zigong, Sichuan, China. FAU - Li, Qiang AU - Li Q AD - Department of Anesthesiology, Zigong Fourth People's Hospital, Zigong, Sichuan, China. FAU - Lu, Bin AU - Lu B AD - Department of Anesthesiology, Zigong Fourth People's Hospital, Zigong, Sichuan, China. FAU - Chen, Guan-Yu AU - Chen GY AD - Department of Anesthesiology, Zigong Fourth People's Hospital, Zigong, Sichuan, China. LA - eng PT - Journal Article PT - Meta-Analysis PT - Systematic Review DEP - 20261014 PL - Switzerland TA - Front Cell Infect Microbiol JT - Frontiers in cellular and infection microbiology JID - 101585359 RN - 0 (Biomarkers) RN - 0 (Leukocyte L1 Antigen Complex) RN - 0 (Calgranulin A) RN - 0 (Calgranulin B) RN - 0 (S100A8 protein, human) RN - 0 (S100A9 protein, human) SB - IM MH - Humans MH - *Biomarkers/blood MH - *Leukocyte L1 Antigen Complex/blood MH - *Sepsis/diagnosis/blood MH - *Calgranulin A/blood MH - Prognosis MH - *Calgranulin B/blood MH - Sensitivity and Specificity MH - Adult MH - ROC Curve PMC - PMC13713635 OTO - NOTNLM OT - S100A8/A9 OT - acute infection OT - biomarker OT - calprotectin OT - critical care OT - diagnostic accuracy OT - meta-analysis OT - sepsis COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:23 PHST- 2026/07/10 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:23 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fcimb.2026.1934020 [doi] PST - epublish SO - Front Cell Infect Microbiol. 2026 Oct 14;16:1934020. doi: 10.3389/fcimb.2026.1934020. eCollection 2026. PMID- 42952924 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2673-7647 (Electronic) IS - 2673-7647 (Linking) VI - 6 DP - 2026 TI - Gene functions determine stochastic or adaptive futile transcription in cancers through deregulated start site deployments. PG - 1883461 LID - 10.3389/fbinf.2026.1883461 [doi] LID - 1883461 AB - Cancers display near-universal hallmarks, including energy addiction, aerobic glycolysis, and biosynthesis. In addition to glucose (and glutamine) addiction, cancers display futile transcription. Discretely measurable futile transcription occurs between transcription start sites (TSSs) and start codons of protein-coding genes. Multiple TSSs for each gene offer various combinations of futile transcription with no effect on the encoded proteins. The relationship between proximal and distal TSS deployment, futile transcription, and energy addiction of cancers remains unclear. By analyzing CAGE and PRO-cap data, we show that TSS deployment dysregulation in cancers increases the energy cost coefficients of cancer transcriptomes. We define the bases of the altered energy cost coefficients of cancer transcriptomes by comparing TSS deployment frequencies, associated entropies, and futile transcription distances across heterogeneous pools of cancers and non-cancers. We show that TSS deployment entropies differ between cancers and non-cancers. It leads to an overall higher distal TSS deployment in cancers but selectively favors an energy-economical proximal TSS deployment for cancer hallmark genes involved in cell proliferation and biosynthesis. We show that the frequency of TSS deployment is linked differently to futile transcription distance and gene function in cancers and non-cancers. This work lays out a theoretical framework describing the stochasticity of TSS deployment in the context of cancerogenesis and the energetics of transcription. It also suggests that the current human TSS landscape has evolved to minimize futile transcription, an outcome favored by stochastic TSS deployment. Under normal conditions of energy metabolism, it forms a threshold that incipient cancer cells breach. CI - Copyright (c) 2026 Satish, Kumar, Morbia and Singh. FAU - Satish, Aditi Lakshmi AU - Satish AL AD - Department of Biological Sciences and Engineering, HoMeCell Lab, Indian Institute of Technology Gandhinagar, Gandhinagar, India. FAU - Kumar, Praveen AU - Kumar P AD - Department of Biological Sciences and Engineering, HoMeCell Lab, Indian Institute of Technology Gandhinagar, Gandhinagar, India. FAU - Morbia, Ishani AU - Morbia I AD - Department of Biological Sciences and Engineering, HoMeCell Lab, Indian Institute of Technology Gandhinagar, Gandhinagar, India. FAU - Singh, Umashankar AU - Singh U AD - Department of Biological Sciences and Engineering, HoMeCell Lab, Indian Institute of Technology Gandhinagar, Gandhinagar, India. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Bioinform JT - Frontiers in bioinformatics JID - 9918227263306676 PMC - PMC13713664 OTO - NOTNLM OT - TSS distance OT - alternative TSS OT - cancer OT - gene function OT - stochastic OT - transcriptional cost COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:23 PHST- 2026/05/17 00:00 [received] PHST- 2026/08/07 00:00 [revised] PHST- 2026/08/11 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:23 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1883461 [pii] AID - 10.3389/fbinf.2026.1883461 [doi] PST - epublish SO - Front Bioinform. 2026 Oct 14;6:1883461. doi: 10.3389/fbinf.2026.1883461. eCollection 2026. PMID- 42952920 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Intratumoral susceptibility signals in adult-type diffuse gliomas: associations with molecular profile, tumor grade, and overall survival. PG - 1905588 LID - 10.3389/fmed.2026.1905588 [doi] LID - 1905588 AB - PURPOSE: Intratumoral Susceptibility Signals (ITSS) are low-signal-intensity structures observed on susceptibility-weighted imaging (SWI) which may reflect intratumoral microhemorrhages, calcifications, and neovascularization. Previous studies have demonstrated associations between ITSS, glioma grade, and molecular characteristics; however, their potential role in outcome prediction remains incompletely defined. This study investigated the diagnostic and and potential prognostic role of ITSS in adult diffuse gliomas. METHODS: We retrospectively analyzed 128 adult patients with histologically confirmed diffuse gliomas who underwent preoperative MRI including SWI. ITSS were graded from 0 to 3 according to the number of susceptibility signals. Associations between ITSS grade and CNS WHO tumor grade, IDH mutation status, MGMT promoter methylation and Ki-67 labeling index (LI) were evaluated. Overall survival (OS) was assessed using Kaplan-Meier analysis and Cox proportional hazards models. RESULTS: ITSS grade was significantly associated with WHO glioma grade (p < 0.001), with lower-grade tumors predominantly exhibiting ITSS grades 0-1 and higher-grade gliomas showing grades 2-3. IDH-mutant gliomas displayed significantly lower ITSS grades than IDH-wildtype tumors (p < 0.001). ITSS grade was positively correlated with Ki-67 labeling index (Spearman's rho = 0.423, p < 0.001). In the survival cohort (n = 87) higher ITSS burden was significantly associated with shorter OS (ITSS 2-3 vs. 0-1: HR = 3.19, 95% CI 1.65-6.19; p < 0.001). However, this association was no longer significant after adjustment for established prognostic factors (HR = 1.40, 95% CI 0.69-2.86; p = 0.350), with IDH status emerging as the strongest independent association with shorter OS. CONCLUSION: ITSS grading on SWI is associated with CNS WHO grade, molecular profile, proliferative activity, and OS in adult-type diffuse gliomas. Although ITSS was not an independent predictor of survival after adjustment for established prognostic factors, it may serve as a readily available, non-invasive imaging correlate of tumor aggressiveness, providing complementary information for preoperative tumor characterization while definitive molecular data are pending. CI - (c) 2026 Feraco, Giuliani, Espa, Gagliardo, Henssen, Meijer, Greco, Bertalot and Caffo. FAU - Feraco, Paola AU - Feraco P AD - Dipartimento di Medicina e Scienze della Salute, University of Trento, Trento, Italy. AD - Neuroradiology Unit, Santa Chiara Hospital, ASUIT, Trento, Italy. FAU - Giuliani, Diego AU - Giuliani D AD - Department of Economy and Management, University of Trento, Trento, Italy. FAU - Espa, Giuseppe AU - Espa G AD - Department of Economy and Management, University of Trento, Trento, Italy. AD - Radiomics Laboratory, Department of Economy and Management, University of Trento, Trento, Italy. FAU - Gagliardo, Cesare AU - Gagliardo C AD - Department of Biomedicine, Neurosciences and Advanced Diagnostics (BIND), University of Palermo, Palermo, Italy. FAU - Henssen, Dylan AU - Henssen D AD - Department of Medical Imaging, Radboud University Medical Center, Nijmegen, Netherlands. AD - Department of Nuclear Medicine, University Hospital Leipzig, Leipzig, Germany. FAU - Meijer, Frederick J A AU - Meijer FJA AD - Department of Medical Imaging, Radboud University Medical Center, Nijmegen, Netherlands. FAU - Greco, Valentina AU - Greco V AD - Medical Oncology Department, Santa Chiara Hospital, ASUIT, Trento, Italy. FAU - Bertalot, Giovanni AU - Bertalot G AD - Dipartimento di Medicina e Scienze della Salute, University of Trento, Trento, Italy. AD - Medical Oncology Department, Santa Chiara Hospital, ASUIT, Trento, Italy. FAU - Caffo, Orazio AU - Caffo O AD - Dipartimento di Medicina e Scienze della Salute, University of Trento, Trento, Italy. AD - Surgical Pathology, Santa Chiara Hospital, ASUIT, Trento, Italy. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13713659 OTO - NOTNLM OT - MRI OT - glioma OT - intratumoral susceptibility signals (ITSS) OT - isocitrate dehydrogenase OT - radiogenomic COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The authors PF, DH declared that they were an editorial board member of Frontiers at the time of submission. This had no impact on the peer review process and the final decision. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:23 PHST- 2026/06/10 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:23 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmed.2026.1905588 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 14;13:1905588. doi: 10.3389/fmed.2026.1905588. eCollection 2026. PMID- 42952915 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-1078 (Print) IS - 1664-1078 (Electronic) IS - 1664-1078 (Linking) VI - 17 DP - 2026 TI - Animation-assisted psychological intervention in pediatric care: mechanisms, clinical applications, and emerging evidence. PG - 1969526 LID - 10.3389/fpsyg.2026.1969526 [doi] LID - 1969526 AB - Children frequently experience fear, anxiety, and pain during medical encounters, and this distress can lead to poor cooperation, longer procedures, and lasting avoidance of care. Animation, delivered as cartoons, preparation films, applications, serious games, and virtual reality (VR) scenes, has become one of the most widely deployed non-pharmacological adjuncts in pediatric settings because it is inexpensive, non-invasive, and easily standardized. Drawing on a structured search of six databases through July 2026, this mini-review synthesizes randomized and meta-analytic evidence across perioperative anxiety, procedural pain, pediatric dentistry, chronic-disease self-management, and mental health promotion. It organizes the evidence around five psychological pathways: attentional competition, observational learning, exposure and inhibitory learning, narrative-affective processing, and self-efficacy. Additionally, it examines a sixth, non-psychological use in which animation is used to provide therapeutic visual stimulation. Evidence supports moderate short-term benefits for anticipatory anxiety and for needle- and dentistry-related distress, with weaker and less consistent effects for high-intensity procedures and physiological outcomes. We argue that the field's core issue is conceptual rather than empirical: animation refers to a delivery medium, not a treatment, and trials have compared media rather than mechanisms. This obscures three unresolved problems: discordance between self-reported and autonomic outcomes; an untheorized moderating role of the developmental stage; and a theoretical tension between distraction, which withdraws attention from the feared stimulus, and exposure, which requires engagement with it. Mechanism-matched comparators, core outcome sets, developmentally stratified designs, transparent intervention reporting, and follow-up across repeated procedures are prerequisites for cumulative progress. CI - Copyright (c) 2026 Dongxian, Zeqi, Qi and Shutong. FAU - Dongxian, Wu AU - Dongxian W AD - Hanseo University, Seosan-si, Republic of Korea. FAU - Zeqi, Yu AU - Zeqi Y AD - Royal College of Art, London, United Kingdom. FAU - Qi, Shu AU - Qi S AD - Royal College of Art, London, United Kingdom. FAU - Shutong, Wang AU - Shutong W AD - Hanseo University, Seosan-si, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Psychol JT - Frontiers in psychology JID - 101550902 PMC - PMC13714181 OTO - NOTNLM OT - animation OT - children OT - dental anxiety OT - distraction OT - mental health literacy OT - preoperative anxiety OT - procedural pain OT - virtual reality COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:23 PHST- 2026/08/16 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:23 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpsyg.2026.1969526 [doi] PST - epublish SO - Front Psychol. 2026 Oct 14;17:1969526. doi: 10.3389/fpsyg.2026.1969526. eCollection 2026. PMID- 42952913 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-861X (Print) IS - 2296-861X (Electronic) IS - 2296-861X (Linking) VI - 13 DP - 2026 TI - From yield to nutrition: re-imagining crop nutritional resilience for global nutrition security under climate change. PG - 1987519 LID - 10.3389/fnut.2026.1987519 [doi] LID - 1987519 AB - Climate change is increasingly challenging crop-based food and nutrition security not only through reductions in crop productivity but also by altering the nutritional composition, bioavailability, and functionality properties of food. Rising temperature, drought, excessive rainfall and flooding, elevated atmospheric CO(2), and salinity can modify the accumulation, partitioning, and availability of proteins, minerals, vitamins, carotenoids, phenolics, flavonoids, and other bioactive compounds in crop-derived foods. Elevated CO(2) is frequently associated with dilution of protein, iron (Fe), zinc (Zn), and other minerals in crops while heat and water stress can further disrupt nutrient uptake, translocation, and grain filling. However, responses are not universally negative: moderate environmental stress may stimulate selected phytochemicals and antioxidants, and responses depend strongly on crop species, genotype, nutrient, stress intensity and duration, developmental stage, and environmental context. Nutrient concentration alone also does not determine nutritional value because anti-nutritional factors, food matrix characteristics, processing, digestibility, and bioavailability influence the nutrients ultimately available to consumers. This mini-review synthesizes the evidence on climate-related changes in crop nutritional quality and develops the concept of nutritional resilience around four interconnected dimensions: productivity, nutrient density, bioavailability, and dietary diversity. The framework is intended to complement, not replace, existing food security, nutrition-sensitive agriculture, and climate-resilient approaches by emphasizing whether climate adaptation can maintain nutritionally meaningful food from crop production through consumption. CI - Copyright (c) 2026 Dissanayaka. FAU - Dissanayaka, D M S B AU - Dissanayaka DMSB AD - Department of Crop Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, Sri Lanka. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Front Nutr JT - Frontiers in nutrition JID - 101642264 PMC - PMC13714179 OTO - NOTNLM OT - biofortification OT - climate change OT - food systems OT - hidden hunger OT - micronutrients OT - nutrient density COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:22 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:22 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fnut.2026.1987519 [doi] PST - epublish SO - Front Nutr. 2026 Oct 14;13:1987519. doi: 10.3389/fnut.2026.1987519. eCollection 2026. PMID- 42952911 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2094-9278 (Electronic) IS - 0001-6071 (Print) IS - 0001-6071 (Linking) VI - 60 IP - 17 DP - 2026 TI - Comparison of Single Drop versus Two Drops 0.5% Tropicamide Using Manual and Automated Refraction in Children Aged 5-7 Years: A Randomized Controlled Trial. PG - 9-17 LID - 10.47895/amp.v60i17.14500 [doi] AB - BACKGROUND: Cycloplegia is important for obtaining accurate refractive measurements in children. However, the limited availability of cycloplegic agents in the Philippines presents challenges for pediatric mass-refraction programs. OBJECTIVE: This study evaluated the efficacy and reliability of 0.5% tropicamide administered as a single-drop versus the standard two-drop regimen for obtaining cycloplegic refraction in children. METHODS: This multicenter, randomized, single-sequence, crossover, controlled, single-blind study included children aged 5-7 years recruited from selected schools and a pediatric ophthalmology clinic in a tertiary hospital in the Philippines. Participants were randomized to receive a single drop and two drops of 0.5% tropicamide according to the study sequence. Automated refraction was performed at 5, 15, and 30 minutes, followed by manual refraction at 30-40 minutes. Another manual refraction performed 30-40 minutes after 2 drops of 0.5% tropicamide served as the reference standard. The pediatric ophthalmologists performing the reference refraction were masked to the automated refraction results. RESULTS: A total of 219 eyes from 111 children were included, 59% (66/111) of whom were male. Hyperopia was the most common refractive error (76%, 166/219), followed by astigmatism (62.6%, 137/219) and myopia (16%, 34/219). After a single drop of tropicamide, auto-mated refraction at 5, 15, and 30 minutes demonstrated significant differences from the reference standard in spherical equivalent (SE), sphere, cylinder, and axis (all P <0.05). The median SE values at 5, 15, and 30 minutes were 0.25 D (Interquartile Range or IQR, 1.25 D), 0.25 D (IQR, 1.50 D), and 0.375 D (IQR, 1.50 D), respectively, compared with 0.75 D (IQR, 1.75 D) for the reference refraction. In contrast, manual refraction performed 30-40 minutes after a single drop did not differ significantly from the reference standard for sphere (P = 0.479), cylinder (P = 0.470), axis (P = 0.137), or SE (P = 0.327). Following two drops, automated refraction at all time points remained significantly different from the reference standard for cylinder, axis, and SE. However, at 30 minutes, the spherical refraction did not differ significantly from the reference standard (P = 0.209). CONCLUSION: In children aged 5-7 years, manual refraction performed 30-40 minutes after either a single- or two-drop administration of 0.5% tropicamide produced results comparable with the reference standard. Automated refraction following a single drop demonstrated greater variability and should be interpreted with caution. For mass-refraction settings, a single-drop regimen followed by manual refraction after 30-40 minutes may be a practical alternative to the standard two-drop regimen. When automated refraction is used, the sphere obtained after two drops may provide an estimate of cycloplegic refraction. CI - (c) The Author(s) 2026. FAU - Torrefranca, Aramis B Jr AU - Torrefranca AB Jr AD - Department of Ophthalmology and Visual Sciences, Philippine General Hospital, University of the Philippines Manila, Manila, Philippines. AD - Department of Ophthalmology, Department of Health - Governor Celestino Gallares Memorial Medical Center, Bohol, Philippines. FAU - Santiago, Alvina Pauline D AU - Santiago APD AD - Department of Ophthalmology and Visual Sciences, Philippine General Hospital, University of the Philippines Manila, Manila, Philippines. AD - Eye and Vision Institute, The Medical City, Pasig City, Philippines. AD - International Eye Institute, St. Luke's Medical Center, Quezon City, Philippines. AD - Department of Ophthalmology, Manila Doctors Hospital, Manila, Philippines. FAU - Paulino, Jose Antonio T AU - Paulino JAT AD - Department of Ophthalmology, Asian Hospital and Medical Center, Muntinlupa City, Philippines. AD - Olivarez General Hospital, Paranaque City, Philippines. FAU - Astudillo, Paulita Pamela P AU - Astudillo PPP AD - Department of Ophthalmology and Visual Sciences, Philippine General Hospital, University of the Philippines Manila, Manila, Philippines. AD - Department of Ophthalmology, Jose B. Lingad Memorial Medical Center, Pampanga, Philippines. FAU - Monzon-Pajarillo, Andrea Kristina AU - Monzon-Pajarillo AK AD - Department of Ophthalmology and Visual Sciences, Philippine General Hospital, University of the Philippines Manila, Manila, Philippines. FAU - Valbuena, Marissa N AU - Valbuena MN AD - Department of Ophthalmology and Visual Sciences, Philippine General Hospital, University of the Philippines Manila, Manila, Philippines. LA - eng PT - Journal Article DEP - 20260915 PL - Philippines TA - Acta Med Philipp JT - Acta medica Philippina JID - 0312677 PMC - PMC13713872 OTO - NOTNLM OT - Philippines OT - autorefraction OT - manual refraction OT - single drop OT - tropicamide COIS- All authors declared no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/15 CRDT- 2026/10/29 05:22 PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:22 [entrez] PHST- 2026/09/15 00:00 [pmc-release] AID - AMP-60-17-14500 [pii] AID - 10.47895/amp.v60i17.14500 [doi] PST - epublish SO - Acta Med Philipp. 2026 Sep 15;60(17):9-17. doi: 10.47895/amp.v60i17.14500. eCollection 2026. PMID- 42952907 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2094-9278 (Electronic) IS - 0001-6071 (Print) IS - 0001-6071 (Linking) VI - 60 IP - 17 DP - 2026 TI - Modified Three-port Approach for Absolute Alcohol-induced Sclerosis of Iris Stromal Cyst: A Case Report. PG - 91-98 LID - 10.47895/amp.v60i17.13492 [doi] AB - Iris stromal cysts are rare, progressive ocular lesions that can cause visual impairment and amblyopia due to visual axis obstruction, corneal endothelial damage, uveitis, and secondary angle-closure glaucoma. Management is challenging due to recurrence, and surgical resection carries severe risks such as epithelial downgrowth, glaucoma, blindness, and potential loss of the eye. Absolute alcohol-induced sclerosis is a less invasive alternative; however, the literature on technique modifications remains limited. This case report describes the successful management of a primary iris stromal cyst with secondary ocular hypertension in a 9-year-old Filipino male using a modified three-port approach for absolute alcohol-induced sclerosis. The patient presented with a 4-month history of intermittent redness and pain in the left eye and was found to have elevated intraocular pressure (IOP) due to an iris stromal cyst. A modified three-port setup was prepared using a 30-gauge needle connected to a 3-way stopcock, a 3-mL syringe for aspiration, and an extension tube connected to a syringe containing absolute alcohol mixed with trypan blue dye. Although a 3-mL syringe was used for alcohol infusion, a 1-mL syringe may provide finer control. Postoperatively, transient corneal edema, mild anterior chamber inflammation, and steroid-induced intraocular pressure elevation were observed but resolved with medical management. At 18 months post-surgery, the patient remained asymptomatic, with a best-corrected visual acuity of 20/20, well-controlled IOP without medication, and no cyst recurrence. This case illustrates the effectiveness of a modified three-port approach in treating iris stromal cysts while preventing complications such as visual axis obstruction, glaucoma, and the need for more invasive surgery. This technique presents a practical and accessible option, especially in resource-limited settings. The paper also emphasizes the importance of meticulous technique and awareness of alcohol leakage, with key precautions outlined for safe application. CI - (c) The Author(s) 2026. FAU - Santos, Bobbie Marie M AU - Santos BMM AD - Department of Ophthalmology and Visual Sciences, Philippine General Hospital, University of the Philippines Manila, Manila, Philippines. FAU - Rivera, Jayvee S AU - Rivera JS AD - Department of Ophthalmology and Visual Sciences, Philippine General Hospital, University of the Philippines Manila, Manila, Philippines. FAU - Sosuan, George Michael N AU - Sosuan GMN AD - Department of Ophthalmology and Visual Sciences, Philippine General Hospital, University of the Philippines Manila, Manila, Philippines. FAU - Tan, Reginald Robert G AU - Tan RRG AD - Department of Ophthalmology and Visual Sciences, Philippine General Hospital, University of the Philippines Manila, Manila, Philippines. AD - Eye Institute, St. Luke's Medical Center, Quezon City, Philippines. FAU - Siong, Ruben Lim Bon AU - Siong RLB AD - Department of Ophthalmology and Visual Sciences, Philippine General Hospital, University of the Philippines Manila, Manila, Philippines. AD - Eye Institute, St. Luke's Medical Center, Quezon City, Philippines. LA - eng PT - Case Reports PT - Journal Article DEP - 20260915 PL - Philippines TA - Acta Med Philipp JT - Acta medica Philippina JID - 0312677 PMC - PMC13713870 OTO - NOTNLM OT - absolute alcohol OT - case report OT - iris cyst OT - ocular hypertension COIS- All authors declared no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/09/15 CRDT- 2026/10/29 05:22 PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:22 [entrez] PHST- 2026/09/15 00:00 [pmc-release] AID - AMP-60-17-13492 [pii] AID - 10.47895/amp.v60i17.13492 [doi] PST - epublish SO - Acta Med Philipp. 2026 Sep 15;60(17):91-98. doi: 10.47895/amp.v60i17.13492. eCollection 2026. PMID- 42952905 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 0970-9134 (Print) IS - 0973-7723 (Electronic) IS - 0970-9134 (Linking) VI - 42 IP - 11 DP - 2026 Nov TI - Atrial septal defect associated with suspected left ventricular non-compaction supported by histopathological findings in a child. PG - 1229-1233 LID - 10.1007/s12055-026-02241-5 [doi] AB - Left ventricular non-compaction (LVNC) is a rare cardiomyopathy characterized by excessive trabeculation of the left ventricular myocardium. The debate over whether LVNC is a distinct disease entity limits the estimates on its prevalence and incidence as there is a significant overlap with other known cardiomyopathies like hypertrophic cardiomyopathy, apical hypertrophy, and dilated cardiomyopathy. This condition is defined by excessive trabeculation of the myocardium, with deep intertrabecular recesses that communicate directly with the ventricular cavity. We report an 11-year-old boy with ostium secundum atrial septal defect (ASD), unexplained left ventricular dysfunction, and intraoperative findings suggestive of LVNC, in whom histopathological examination provided supportive evidence for the diagnosis. The decision for fenestrated ASD closure was based on reduced left ventricular compliance demonstrated during preoperative assessment. This case highlights the diagnostic challenges of LVNC when classical echocardiographic criteria are not fulfilled and emphasizes the complementary role of histopathological findings in selected cases. CI - (c) Indian Association of Cardiovascular-Thoracic Surgeons 2026. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. FAU - Osama, Md Ali AU - Osama MA AUID- ORCID: 0000-0002-0660-9939 AD - Department of Pathology, All India Institute of Medical Sciences, New Delhi, 110029 India. ROR: https://ror.org/02dwcqs71. GRID: grid.413618.9. ISNI: 0000 0004 1767 6103 FAU - Malick, Sunil Kumar AU - Malick SK AD - Department of Pathology, All India Institute of Medical Sciences, New Delhi, 110029 India. ROR: https://ror.org/02dwcqs71. GRID: grid.413618.9. ISNI: 0000 0004 1767 6103 FAU - Sargunan, Bala Brindha AU - Sargunan BB AUID- ORCID: 0000-0002-0258-5941 AD - Department of Cardiothoracic and Vascular Surgery, All India Institute of Medical Sciences, New Delhi, India. ROR: https://ror.org/02dwcqs71. GRID: grid.413618.9. ISNI: 0000 0004 1767 6103 FAU - Varghese, Mariya Biji AU - Varghese MB AD - Department of Pathology, All India Institute of Medical Sciences, New Delhi, 110029 India. ROR: https://ror.org/02dwcqs71. GRID: grid.413618.9. ISNI: 0000 0004 1767 6103 FAU - Rajashekar, Palleti AU - Rajashekar P AUID- ORCID: 0000-0003-1644-9964 AD - Department of Cardiothoracic and Vascular Surgery, All India Institute of Medical Sciences, New Delhi, India. ROR: https://ror.org/02dwcqs71. GRID: grid.413618.9. ISNI: 0000 0004 1767 6103 FAU - Arava, Sudheer AU - Arava S AD - Department of Pathology, All India Institute of Medical Sciences, New Delhi, 110029 India. ROR: https://ror.org/02dwcqs71. GRID: grid.413618.9. ISNI: 0000 0004 1767 6103 FAU - Ray, Ruma AU - Ray R AD - Department of Pathology, All India Institute of Medical Sciences, New Delhi, 110029 India. ROR: https://ror.org/02dwcqs71. GRID: grid.413618.9. ISNI: 0000 0004 1767 6103 LA - eng PT - Case Reports PT - Journal Article DEP - 20260717 PL - India TA - Indian J Thorac Cardiovasc Surg JT - Indian journal of thoracic and cardiovascular surgery JID - 8700105 PMC - PMC13713928 OTO - NOTNLM OT - Atrial septal defect OT - Congenital heart disease OT - Fenestrated ASD closure OT - Left ventricular non-compaction OT - Pediatric cardiomyopathy COIS- Conflict of interestThe authors declare that there are no potential conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2027/11/01 CRDT- 2026/10/29 05:22 PHST- 2026/03/02 00:00 [received] PHST- 2026/06/22 00:00 [revised] PHST- 2026/06/27 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:22 [entrez] PHST- 2027/11/01 00:00 [pmc-release] AID - 2241 [pii] AID - 10.1007/s12055-026-02241-5 [doi] PST - ppublish SO - Indian J Thorac Cardiovasc Surg. 2026 Nov;42(11):1229-1233. doi: 10.1007/s12055-026-02241-5. Epub 2026 Jul 17. PMID- 42952898 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2432-3853 (Electronic) IS - 2432-3853 (Linking) VI - 10 IP - 4 DP - 2026 TI - Risk of Advanced Colorectal Neoplasia among Patients Undergoing Repeated Colonoscopy Surveillance: A Two-Center Study. PG - 642-650 LID - 10.23922/jarc.2026-047 [doi] AB - OBJECTIVES: Postpolypectomy surveillance plays an important role in the prevention of colorectal cancer. However, optimal surveillance intervals for individuals with multiple prior colonoscopies remain unclear. This study aimed to evaluate the association between prior colonoscopy frequency and advanced neoplasia detection rate (ANDR) according to cumulative colorectal neoplasia treatment history using real-world data from individuals with multiple prior colonoscopies. METHODS: This two-center retrospective study analyzed colonoscopies performed between 2010 and 2020 in patients aged >/=40 years. The number of colonoscopies performed within the preceding five years was routinely recorded for each examination. The primary outcome was ANDR. Surveillance intervals were considered appropriate when the ANDR was /=3.07 as optimal cutoffs. CRP and WBC count showed low AUCs (0.560 and 0.580, respectively) and were not significant risk factors for SBO. Univariate analysis demonstrated that low BMI, low LMR, high NLR, stoma construction, and intra-abdominal infectious complications were significantly associated with SBO development. Multivariate logistic regression analysis identified low BMI (p = 0.022), low LMR (p = 0.047), and intra-abdominal infectious complications (p < 0.001) as independent predictors; PNI trend was not significant. CONCLUSIONS: Low BMI, low LMR, and postoperative intra-abdominal infection were associated with postoperative SBO after minimally invasive colon cancer surgery. CI - Copyright (c) 2026 The Japan Society of Coloproctology. FAU - Tamai, Koki AU - Tamai K AD - Department of Gastroenterological Surgery, Osaka Rosai Hospital, Sakai, Osaka, Japan. FAU - Tei, Mitsuyoshi AU - Tei M AD - Department of Gastroenterological Surgery, Osaka Rosai Hospital, Sakai, Osaka, Japan. FAU - Tsujimura, Naoto AU - Tsujimura N AD - Department of Gastroenterological Surgery, Osaka Rosai Hospital, Sakai, Osaka, Japan. FAU - Nishida, Kentaro AU - Nishida K AD - Department of Gastroenterological Surgery, Osaka Rosai Hospital, Sakai, Osaka, Japan. FAU - Mori, Soichiro AU - Mori S AD - Department of Gastroenterological Surgery, Osaka Rosai Hospital, Sakai, Osaka, Japan. FAU - Yoshikawa, Yukihiro AU - Yoshikawa Y AD - Department of Gastroenterological Surgery, Osaka Rosai Hospital, Sakai, Osaka, Japan. FAU - Ohara, Nobuyoshi AU - Ohara N AD - Department of Gastroenterological Surgery, Osaka Rosai Hospital, Sakai, Osaka, Japan. FAU - Hamakawa, Takuya AU - Hamakawa T AD - Department of Gastroenterological Surgery, Osaka Rosai Hospital, Sakai, Osaka, Japan. FAU - Takiuchi, Daisuke AU - Takiuchi D AD - Department of Gastroenterological Surgery, Osaka Rosai Hospital, Sakai, Osaka, Japan. FAU - Tsujie, Masanori AU - Tsujie M AD - Department of Gastroenterological Surgery, Osaka Rosai Hospital, Sakai, Osaka, Japan. FAU - Akamaru, Yusuke AU - Akamaru Y AD - Department of Gastroenterological Surgery, Osaka Rosai Hospital, Sakai, Osaka, Japan. LA - eng PT - Journal Article DEP - 20261025 PL - Japan TA - J Anus Rectum Colon JT - Journal of the anus, rectum and colon JID - 101718055 PMC - PMC13713568 OTO - NOTNLM OT - laparoscopic colon cancer surgery OT - lymphocyte-to monocyte ratio OT - small bowel obstruction COIS- The authors declare that there are no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/25 CRDT- 2026/10/29 05:22 PHST- 2026/04/20 00:00 [received] PHST- 2026/06/10 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:22 [entrez] PHST- 2026/10/25 00:00 [pmc-release] AID - 10.23922/jarc.2026-051 [doi] PST - epublish SO - J Anus Rectum Colon. 2026 Oct 25;10(4):552-560. doi: 10.23922/jarc.2026-051. eCollection 2026. PMID- 42952884 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Case Report: Rectal perforation combined with presacral infection following rubber band ligation and sclerotherapy for hemorrhoids. PG - 1938366 LID - 10.3389/fmed.2026.1938366 [doi] LID - 1938366 AB - BACKGROUND: Currently, rubber band ligation and injection sclerotherapy represent the first-line therapeutic strategy for grade I-III internal hemorrhoids. As an invasive procedure, it may still cause postoperative complications, among which, rectal perforation is one of the most serious complications. CASE PRESENTATION: This paper describes a patient treated at our facility who experienced a rectal perforation and presacral infection following rubber band ligation and injection sclerotherapy. In the first stage, we performed laparoscopic double-barreled transverse colostomy combined with anal irrigation and anal dilatation to achieve fecal diversion and adequate drainage of the infectious focus. The patient achieved a satisfactory postoperative recovery. Stoma reversal was performed 6 months after surgery. CONCLUSION: Rectal perforation combined with presacral infection is a rare but severe complication of rubber band ligation and sclerotherapy for hemorrhoids, among these, sclerotherapy is the primary factor, while rubber band ligation is a contributing factor. It is mostly related to inappropriate iatrogenic operative manipulation. Clinically, strict standardization of rubber band ligation and injection sclerotherapy procedures is required, and close surveillance of high-risk patients after surgery should be strengthened to avoid complications and prevent delayed diagnosis and intervention. CI - (c) 2026 Lu, Wang, Shi, Jiang, Zhang, Gan, Zhou and Wang. FAU - Lu, Yanqing AU - Lu Y AD - The Second Clinical Medical College of Guangzhou University of Chinese Medicine, Guangzhou, China. FAU - Wang, Ziwen AU - Wang Z AD - Department of Coloproctology, The Affiliated Guangzhou Hospital of TCM of Guangzhou University of Chinese Medicine, Guangzhou, China. FAU - Shi, Zhenfu AU - Shi Z AD - Department of Proctology, Yinchuan Hospital of Traditional Chinese Medicine Affiliated to Ningxia Medicine University, Yinchuan, China. FAU - Jiang, Zhi AU - Jiang Z AD - Department of Colorectal Surgery, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China. FAU - Zhang, Li AU - Zhang L AD - Department of Colorectal Surgery, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China. FAU - Gan, Guolian AU - Gan G AD - Department of Colorectal Surgery, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China. FAU - Zhou, Zhen AU - Zhou Z AD - Department of Colorectal Surgery, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China. FAU - Wang, Hao AU - Wang H AD - Department of Colorectal Surgery, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China. LA - eng PT - Case Reports PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13713612 OTO - NOTNLM OT - anal stenosis OT - case report OT - fecal diversion OT - injection sclerotherapy OT - presacral infection OT - rectal perforation OT - rubber band ligation COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:21 PHST- 2026/07/15 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:21 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmed.2026.1938366 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 14;13:1938366. doi: 10.3389/fmed.2026.1938366. eCollection 2026. PMID- 42952875 OWN - NLM STAT- Publisher LR - 20261029 IS - 1757-1774 (Print) IS - 1757-1782 (Electronic) IS - 1757-1782 (Linking) DP - 2026 Oct 26 TI - Development and content validation of a risk-stratified nursing intervention programme for the prevention of healthcare-associated infections. PG - 17571774261499905 LID - 10.1177/17571774261499905 [doi] LID - 17571774261499905 AB - BACKGROUND: Healthcare-associated infections (HAIs) remain a major patient-safety challenge, and preventive interventions are rarely tailored to individual infection risk. This study aimed to develop and content-validate a risk-stratified nursing intervention programme for preventing HAIs in hospitalised adults. METHODS: A methodological Delphi study was conducted with nursing experts in infection prevention and control. An evidence-informed preliminary programme was evaluated for relevance, clarity, appropriateness to risk level and feasibility. Twenty-two experts were invited; 14 participated in round one and 10 in round two. Consensus was assessed using predefined median, interquartile range and agreement criteria. RESULTS: The preliminary programme comprised 26 nursing interventions organised by infection risk level. In round one, 18 interventions (69.2%) were retained, seven were revised and one was repositioned as a monitoring strategy. In round two, four revised interventions met all predefined consensus criteria. Routine chlorhexidine use for moderate-risk patients was not retained, whereas conventional body washing and chlorhexidine use for selected high-risk situations were retained following integrated quantitative and qualitative assessment. The final programme integrates universal and risk-specific interventions, periodic risk reassessment, discharge planning, continuity of care and quality monitoring. CONCLUSIONS: The Delphi process established the content validity of a risk-stratified nursing intervention programme for HAI prevention. The programme provides a structured framework to support nurses in translating infection risk assessment into tailored preventive interventions, reassessment and continuity of care. Feasibility and clinical effectiveness require evaluation in routine practice. CI - (c) The Author(s) 2026. FAU - Todo Bom, Luis Filipe Pereira AU - Todo Bom LFP AUID- ORCID: 0000-0001-6739-8648 AD - Comprehensive Health Research Centre (CHRC), University of Evora, Evora, Portugal. AD - Center for Innovative Care and Health Technology (ciTechCare), Leiria, Portugal. AD - University of Leiria and Oeste, Leiria, Portugal. FAU - Dixe, Maria Dos Anjos Coelho Rodrigues AU - Dixe MDACR AUID- ORCID: 0000-0001-9035-8548 AD - Center for Innovative Care and Health Technology (ciTechCare), Leiria, Portugal. AD - University of Leiria and Oeste, Leiria, Portugal. FAU - Marques, Maria do Ceu AU - Marques MDC AUID- ORCID: 0000-0003-2658-3550 AD - Comprehensive Health Research Centre (CHRC), University of Evora, Evora, Portugal. AD - Nursing Department, University of Evora, Evora, Portugal. LA - eng PT - Journal Article DEP - 20261026 PL - England TA - J Infect Prev JT - Journal of infection prevention JID - 101469725 PMC - PMC13713896 OTO - NOTNLM OT - Delphi study OT - healthcare-associated infections OT - infection prevention and control OT - nursing interventions OT - patient safety OT - risk assessment COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:20 PMCR- 2027/10/26 CRDT- 2026/10/29 05:21 PHST- 2026/08/24 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:21 [entrez] PHST- 2027/10/26 00:00 [pmc-release] AID - 10.1177_17571774261499905 [pii] AID - 10.1177/17571774261499905 [doi] PST - aheadofprint SO - J Infect Prev. 2026 Oct 26:17571774261499905. doi: 10.1177/17571774261499905. PMID- 42952873 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Cardiometabolic biomarker profiles associated with estimated 10-year ASCVD risk by the China-PAR model in an older adult health examination cohort: a cross-sectional study. PG - 1897876 LID - 10.3389/fmed.2026.1897876 [doi] LID - 1897876 AB - OBJECTIVE: To characterize the associations between cardiometabolic biomarker profiles and estimated 10-year atherosclerotic cardiovascular disease (ASCVD) risk calculated using the China-PAR (Prediction for Atherosclerotic Cardiovascular Disease Risk in China) equation in adults aged >/=60 years undergoing routine physical examinations. METHODS: Participants aged >/=60 years were recruited from a hospital health examination center. Ten-year ASCVD risk was calculated using the China-PAR equation and classified as low-to-intermediate (<10%) or high (>/=10%). Associations between continuous biomarkers and the risk score were assessed using Spearman correlation with false discovery rate correction. Exploratory LASSO logistic regression was used for feature selection for China-PAR-defined high-risk status, followed by mutually adjusted logistic regression and quartile-stratified analyses. RESULTS: A total of 511 participants were included (median age 67 years; 62.8% male), and 191 (37.4%) were classified as high risk by China-PAR. Waist-to-hip ratio (WHR; rho = 0.43), fasting plasma glucose (FPG; rho = 0.40), and cystatin C (CysC; rho = 0.39) showed the strongest correlations with the calculated risk score. Exploratory LASSO feature selection retained CysC, FPG, atherogenic coefficient (AC), and N-terminal pro-B-type natriuretic peptide (NT-proBNP). Per one-standard-deviation increase, FPG [adjusted odds ratio [aOR] = 2.772, 95% confidence interval [CI]: 2.047-3.753] and CysC (aOR = 2.731, 95% CI: 1.881-3.964) showed the strongest associations with China-PAR-defined high-risk status. All four biomarkers showed graded trends across quartiles in the overall population and in descriptive sex-stratified analyses. CONCLUSIONS: CysC, FPG, AC, and NT-proBNP were associated with higher estimated China-PAR risk. Because FPG and AC overlap clinically or mathematically with variables used to construct China-PAR, their associations should be interpreted partly in the context of score construction. CysC and NT-proBNP are not components of the China-PAR equation and warrant prospective evaluation. These findings characterize biomarker profiles associated with higher estimated cardiovascular risk and may help increase clinical awareness of unfavorable cardiometabolic and cardiorenal features during routine health examinations. CI - (c) 2026 Zhang, Chen, Zhou and Xiao. FAU - Zhang, LiYing AU - Zhang L AD - Department of Clinical Laboratory, The First People's Hospital of Yibin, Yibin, Sichuan, China. FAU - Chen, Pu AU - Chen P AD - Department of Clinical Laboratory, The Third People's Hospital of Yibin City, Yibin, Sichuan, China. FAU - Zhou, WenTao AU - Zhou W AD - School of Laboratory Medicine, North Sichuan Medical College, Nanchong, Sichuan, China. FAU - Xiao, WeiLei AU - Xiao W AD - Department of Clinical Laboratory, The First People's Hospital of Yibin, Yibin, Sichuan, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13713855 OTO - NOTNLM OT - China-PAR OT - NT-proBNP OT - atherogenic coefficient OT - atherosclerotic cardiovascular disease OT - biomarkers OT - cystatin C OT - fasting plasma glucose OT - older adults COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:21 PHST- 2026/06/02 00:00 [received] PHST- 2026/08/28 00:00 [revised] PHST- 2026/08/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:21 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmed.2026.1897876 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 14;13:1897876. doi: 10.3389/fmed.2026.1897876. eCollection 2026. PMID- 42952866 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-2565 (Electronic) IS - 2296-2565 (Linking) VI - 14 DP - 2026 TI - Relative efficacy of different single and combined conservative interventions for adolescent idiopathic scoliosis: a systematic review and network meta-analysis. PG - 1860459 LID - 10.3389/fpubh.2026.1860459 [doi] LID - 1860459 AB - OBJECTIVE: To systematically evaluate the relative efficacy of different single and combined conservative interventions for adolescent idiopathic scoliosis (AIS), and to provide evidence-based support for individualized conservative treatment strategies in clinical practice. METHODS: Randomized controlled trials on conservative interventions for AIS were retrieved from PubMed, Embase, Web of Science, Scopus, and the Cochrane Library from database inception to December 31, 2025. Two reviewers independently performed study selection, data extraction, and risk-of-bias assessment. A network meta-analysis was conducted using Stata 18.0. The main outcomes included Cobb angle, angle of trunk rotation (ATR), and SRS-22 score. The surface under the cumulative ranking curve (SUCRA) was used to rank different interventions. RESULTS: A total of 26 randomized controlled trials were included, involving 1,384 patients with AIS and 59 study arms. The interventions included Schroth exercises, orthotic treatment, physiotherapeutic scoliosis-specific exercises (PSSE), Scientific Exercises Approach to Scoliosis (SEAS), core stabilization exercises, Pilates, and their combined interventions. The results showed that, for Cobb angle, Schroth exercises combined with hippotherapy, orthotic treatment combined with SEAS, and orthotic treatment alone ranked relatively high; for ATR, Schroth exercises combined with suspension training, Schroth exercises alone, and routine exercise ranked relatively high; for SRS-22 score, Schroth exercises combined with suspension training, Schroth exercises alone, and routine exercise showed relatively high ranking probabilities. Pairwise comparisons showed that Schroth exercises were superior to proprioceptive neuromuscular facilitation (PNF) for ATR. According to the GRADE assessment, the certainty of evidence was moderate for Cobb angle and SRS-22, and low for ATR. CONCLUSION: Both single and combined conservative interventions showed certain therapeutic value for AIS. Some combined interventions demonstrated higher relative efficacy probabilities in improving Cobb angle, ATR, and quality of life. Schroth-based interventions were relatively well-represented in the eligible RCT network and showed favorable ranking probabilities for some outcomes; however, these findings should not be extrapolated to all PSSE approaches. SYSTEMATIC REVIEW REGISTRATION: https://www.crd.york.ac.uk/PROSPERO/view/CRD420261369973, identifier: CRD420261369973. CI - Copyright (c) 2026 Cao, Tao, Shu, Xia and Wu. FAU - Cao, Yuanyuan AU - Cao Y AD - School of Exercise and Health, Shanghai University of Sport, Shanghai, China. FAU - Tao, Meng AU - Tao M AD - School of Exercise and Health, Shanghai University of Sport, Shanghai, China. FAU - Shu, Jiachen AU - Shu J AD - Shanghai Educational Center of Science & Art, Shanghai, China. FAU - Xia, Jun AU - Xia J AD - School of Exercise and Health, Shanghai University of Sport, Shanghai, China. FAU - Wu, Weibing AU - Wu W AD - School of Exercise and Health, Shanghai University of Sport, Shanghai, China. LA - eng PT - Journal Article PT - Network Meta-Analysis PT - Systematic Review DEP - 20261014 PL - Switzerland TA - Front Public Health JT - Frontiers in public health JID - 101616579 SB - IM MH - Humans MH - *Scoliosis/therapy MH - Adolescent MH - *Conservative Treatment/methods MH - *Exercise Therapy/methods MH - Treatment Outcome MH - Randomized Controlled Trials as Topic MH - Combined Modality Therapy PMC - PMC13713608 OTO - NOTNLM OT - Schroth exercise OT - adolescent idiopathic scoliosis OT - combined intervention OT - conservative intervention OT - network meta-analysis OT - systematic review COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:20 PHST- 2026/04/20 00:00 [received] PHST- 2026/09/07 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:20 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpubh.2026.1860459 [doi] PST - epublish SO - Front Public Health. 2026 Oct 14;14:1860459. doi: 10.3389/fpubh.2026.1860459. eCollection 2026. PMID- 42952860 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2432-3853 (Electronic) IS - 2432-3853 (Linking) VI - 10 IP - 4 DP - 2026 TI - Laparoscopic Right Hemicolectomy Using a Fluorescent Ureteral Catheter for Ascending Colon Metastasis of Endometrial Cancer in a Patient with Horseshoe Kidney: A Case Report. PG - 658-663 LID - 10.23922/jarc.2026-038 [doi] AB - INTRODUCTION: Horseshoe kidney is a rare congenital anomaly frequently associated with anatomical variations in the vascular and urinary systems. In colorectal surgery, these abnormalities increase the risk of inadvertent injury and require particular attention. CASE PRESENTATION: We report the case of a 61-year-old woman (body mass index 40) who had undergone a radical hysterectomy for endometrial cancer 27 years earlier and had been followed up in our gynecology department. An elevation in tumor marker levels was noted during follow-up, and metastasis of endometrial cancer was suspected in the ascending colon. To minimize the risk of ureteral injury during minimally invasive surgery in an obese patient who had a history of hysterectomy and a horseshoe kidney, laparoscopic right hemicolectomy with fluorescent ureteral navigation was performed. The right ureter was clearly identified using fluorescence during surgery and completely secured. The histological examination revealed endometrial cancer metastasis in the ascending colon. In this patient with obesity and a horseshoe kidney, laparoscopic surgery using a fluorescent ureteral catheter was successful. A review of the relevant literature is also provided herein. CI - Copyright (c) 2026 The Japan Society of Coloproctology. FAU - Yotsumoto, Takuhiro AU - Yotsumoto T AD - Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, Japan. FAU - Kondo, Akihiro AU - Kondo A AD - Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, Japan. FAU - Feng, Dongping AU - Feng D AD - Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, Japan. FAU - Taketani, Hiroshi AU - Taketani H AD - Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, Japan. FAU - Ishikawa, Ryo AU - Ishikawa R AD - Department of Diagnostic Pathology, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, Japan. FAU - Matsukawa, Hiroyuki AU - Matsukawa H AD - Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, Japan. FAU - Nishiura, Bunpei AU - Nishiura B AD - Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, Japan. FAU - Ando, Yasuhisa AU - Ando Y AD - Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, Japan. FAU - Suto, Hironobu AU - Suto H AD - Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, Japan. FAU - Kishino, Takayoshi AU - Kishino T AD - Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, Japan. FAU - Oshima, Minoru AU - Oshima M AD - Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, Japan. FAU - Okano, Keiichi AU - Okano K AD - Department of Gastroenterological Surgery, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa, Japan. LA - eng PT - Case Reports PT - Journal Article DEP - 20261025 PL - Japan TA - J Anus Rectum Colon JT - Journal of the anus, rectum and colon JID - 101718055 PMC - PMC13713533 OTO - NOTNLM OT - endometrial cancer OT - fluorescent ureteral navigation OT - horseshoe kidney OT - minimally invasive surgery OT - ureteral injury COIS- The authors declare that there are no conflicts of interest EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/25 CRDT- 2026/10/29 05:20 PHST- 2026/03/12 00:00 [received] PHST- 2026/06/21 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:20 [entrez] PHST- 2026/10/25 00:00 [pmc-release] AID - 10.23922/jarc.2026-038 [doi] PST - epublish SO - J Anus Rectum Colon. 2026 Oct 25;10(4):658-663. doi: 10.23922/jarc.2026-038. eCollection 2026. PMID- 42952859 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Analysis of the current status and associated factors of innovative leadership among male nurses in China: a cross-sectional study. PG - 1920462 LID - 10.3389/fmed.2026.1920462 [doi] LID - 1920462 AB - OBJECTIVE: To investigate the current state of innovative leadership among male nurses in Grade A, Level 3 hospitals in Henan Province, China, and analyze factors associated with innovative leadership, thereby providing an empirical basis for developing targeted intervention strategies. METHODS: Using convenience sampling, 222 male nurses from eight Grade A, Level 3 hospitals in Henan Province, China, were selected as study participants between February and June 2025. The survey was conducted using a general data questionnaire, the Innovative Leadership Scale, and the Clinical Thinking Diagnostic of Frontline Nurses. RESULTS: The median score of innovative leadership among male nurses was 154.00 (141.00, 168.00). Innovative leadership was positively correlated with critical thinking (r = 0.663, P < 0.01). In an enter multiple linear regression model, job role, number of published papers, participation in innovative leadership conferences/training, and critical thinking were independently associated with innovative leadership (P < 0.05), and the model explained 76.4% of the total variance. CONCLUSION: Male nurses working in tertiary hospitals in Henan Province showed a moderate level of innovative leadership. Nursing managers should monitor male nurses' innovative thinking and practical behaviors, and implement personalized strategies based on associated factors to support the development of innovative leadership and continuous improvement in nursing service quality. CI - (c) 2026 Ma, Chen, Li, Zhu, Zhang, Cao, Kou and Hu. FAU - Ma, Caixia AU - Ma C AD - Pediatric Department, Henan Provincial People's Hospital, Zhengzhou, Henan, China. FAU - Chen, Xin AU - Chen X AD - School of Nursing, Henan Medical University, Xinxiang, Henan, China. FAU - Li, Zhen AU - Li Z AD - Breast Surgery, Henan Provincial People's Hospital, Zhengzhou, Henan, China. FAU - Zhu, Peiyao AU - Zhu P AD - School of Nursing and Health, Henan University, Kaifeng, Henan, China. FAU - Zhang, Mengge AU - Zhang M AD - School of Nursing, Zhengzhou University, Zhengzhou, Henan, China. FAU - Cao, Jingwen AU - Cao J AD - Neurological ICU, Henan Provincial People's Hospital, Zhengzhou, Henan, China. FAU - Kou, Jie AU - Kou J AD - Nursing Department, Henan Provincial People's Hospital, Zhengzhou, Henan, China. FAU - Hu, Hengyu AU - Hu H AD - School of Nursing and Health, Henan University, Kaifeng, Henan, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13713839 OTO - NOTNLM OT - a cross-sectional study OT - associated factors OT - critical thinking OT - innovative leadership OT - male nurse COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:20 PHST- 2026/07/01 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:20 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmed.2026.1920462 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 14;13:1920462. doi: 10.3389/fmed.2026.1920462. eCollection 2026. PMID- 42952844 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-2565 (Electronic) IS - 2296-2565 (Linking) VI - 14 DP - 2026 TI - Associations of cumulative occupational benzene exposure with genotoxic and immunosenescence-related biomarkers: a cross-sectional study. PG - 1972556 LID - 10.3389/fpubh.2026.1972556 [doi] LID - 1972556 AB - BACKGROUND: Chronic benzene (BZ) exposure is a well-established cause of hematological malignancies, but its potential association with immunosenescence-related alterations at occupational exposure levels below current regulatory limits remains unclear. This cross-sectional study investigated the associations of cumulative BZ exposure with genotoxicity, immunosenescence-related, and hematological biomarkers. METHODS: The study included 228 participants: 115 workers directly involved in BZ production, 75 administrative workers from the same factories without direct involvement in BZ production, and 38 external controls without occupational exposure to industrial solvents. BZ exposure was assessed using personal air monitoring, and cumulative exposure dose (CED) was calculated from airborne BZ concentrations and exposure duration. Genomic damage was evaluated using the cytokinesis-block micronucleus (CBMN) and comet assays. Immunosenescence-related and cellular-stress markers included the CD4/CD8 ratio, CD28(-) frequency, p53 expression, gamma-H2AX expression, and mitochondrial DNA copy number. Hematological parameters were assessed using peripheral blood cell counts. Spearman correlation analysis and outcome-appropriate generalized linear models were used to evaluate the associations between CED and the investigated biomarkers. RESULTS: After full multivariable adjustment, higher CED was associated with greater comet tail intensity (MR = 1.0827, p < 0.001) and tail moment (MR = 1.0922, p < 0.001) and with lower CD4/CD8 ratio (MR = 0.9743, p < 0.001) and p53 expression (MR = 0.9739, p < 0.001). CED was associated with higher micronucleus frequency (MNF) in the age- and sex-adjusted model (RR = 1.0137, p = 0.033). After further adjustment for BMI, hypertension, smoking status, and alcohol use, the effect estimate remained positive, but the association did not reach conventional statistical significance (RR = 1.0123, p = 0.053). No statistically significant associations were observed between CED and comet tail length, CD28(-) frequency, gamma-H2AX expression, mitochondrial DNA copy number, WBC, RBC, platelet count, or neutrophil count in the fully adjusted models. CONCLUSION: Cumulative occupational BZ exposure below the applicable regulatory limits was associated with increased comet-assessed DNA damage and selected immune-related alterations, particularly lower CD4/CD8 ratio and p53 expression. The association between CED and MNF remained positive but was not statistically significant after full adjustment. These findings suggest a selective pattern of BZ-associated genotoxic and immune-related alterations rather than generalized hematological toxicity or a uniform immunosenescence phenotype. Prospective studies are needed to determine their long-term clinical and occupational health significance. CI - Copyright (c) 2026 Xu, Ruan, He, Zhang and Sun. FAU - Xu, Wenqian AU - Xu W AD - Department of Party and Mass Affairs, Shanghai Yangpu District Center for Disease Control and Prevention (Shanghai Yangpu District Health Supervision Institute), Shanghai, China. FAU - Ruan, Xiaoying AU - Ruan X AD - Department of Occupational Health, Hangzhou Hospital for Prevention and Treatment of Occupational Disease, Hangzhou, China. FAU - He, Lu AU - He L AD - Shanghai Yangpu District Center for Health Promotion, Shanghai, China. FAU - Zhang, Yaying AU - Zhang Y AD - Department of Environmental and Occupational Health, Shanghai Yangpu District Center for Disease Control and Prevention (Shanghai Yangpu District Health Supervision Institute), Shanghai, China. FAU - Sun, Yun AU - Sun Y AD - Department of Physical Examination, Shanghai Institute of Occupational Disease for Chemical Industry, Shanghai, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Public Health JT - Frontiers in public health JID - 101616579 RN - J64922108F (Benzene) RN - 0 (Biomarkers) SB - IM MH - Humans MH - *Benzene/adverse effects/toxicity MH - Cross-Sectional Studies MH - Biomarkers/blood MH - *Occupational Exposure/adverse effects/analysis MH - Male MH - Adult MH - Female MH - *DNA Damage/drug effects MH - Middle Aged MH - *Immunosenescence/drug effects MH - Comet Assay MH - Micronucleus Tests PMC - PMC13713486 OTO - NOTNLM OT - benzene OT - comet assay OT - genotoxicity OT - immunosenescence OT - micronucleus OT - occupational exposure COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:19 PHST- 2026/08/19 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:19 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpubh.2026.1972556 [doi] PST - epublish SO - Front Public Health. 2026 Oct 14;14:1972556. doi: 10.3389/fpubh.2026.1972556. eCollection 2026. PMID- 42952842 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-462X (Print) IS - 1664-462X (Electronic) IS - 1664-462X (Linking) VI - 17 DP - 2026 TI - Genome sequencing and de novo assembly elucidate the genetic basis of grain quality and fragrance in heirloom rice cultivar Mushk Budji. PG - 1896265 LID - 10.3389/fpls.2026.1896265 [doi] LID - 1896265 AB - BACKGROUND: Mushk Budji is a premium, GI-tagged, heritage rice landrace of Kashmir valley, J&K, India, distinguished by its short-bold grains, strong aroma, and soft texture. While its morphological and biochemical traits have been documented, no dedicated genomic resource has previously been available. Here we report a de novo genome assembly of Mushk Budji with adequate depth. METHODS: We first-time performed reference guided whole-genome sequencing of Mushk Budji using the Illumina NovaSeq 6000 platform, generating 64.97 Gb of raw data. The genome assembly was achieved using SPAdes and scaffolded using RagTag against the Nipponbare reference. Structural and functional annotations were conducted with AUGUSTUS and curated across NCBI, UniProt, and KEGG databases. The haplotype analysis was performed for loci related to quality using a set of contrasting genotypes. RESULTS AND DISCUSSION: The final assembly comprised 378.29 Mb with an N50 of 29.24 Mb and 97.0% BUSCO completeness. A total of 43,434 non-redundant protein-coding genes were identified, with significant enrichment in pathways related to aroma (2-acetyl-1-pyrroline) and starch biosynthesis. Allelic profiling confirmed the characteristic 8-bp deletion in Badh2, conferring fragrance, alongside additional genes shaping quality and adaptability. Unique haplotypes at SSI and RAmy1A, resembling those of temperate japonica variety Koshihikari, were associated with distinct soft and cohesive cooked texture of Mushk Budji. Comparative analyses revealed evolutionary divergence from canonical japonica references at specific loci. Selective signature analyses revealed contrasting evolutionary patterns among major quality genes. SSI showed evidence of balancing selection, whereas GS3 and Badh2 exhibited signatures of strong human-mediated selection, reflecting the historical preference for desirable grain quality and fragrance traits. Genes controlling amylose content and cooking quality (Wx and Alk) were under moderate purifying selection, indicating functional optimization. CONCLUSION: The genomics resource generated in the present study establishes Mushk Budji as a distinct temperate japonica landrace with unique allelic combinations underpinning its aroma, texture and adaptability. The dataset provides a foundation for conservation, breeding and functional genomics, ensuring the sustainable utilization of this heritage rice. CI - Copyright (c) 2026 Khan, Noor-ul-Ain, Reddy, Rafiq, Khan, Mohiddin, Zargar, Hussain, Alie, Sofi and Shikari. FAU - Khan, Raheel Shafeeq AU - Khan RS AD - Division of Genetics & Plant Breeding, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Wadura, J&K, India. FAU - Noor-Ul-Ain AU - Noor-Ul-Ain AD - Division of Genetics & Plant Breeding, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Wadura, J&K, India. FAU - Reddy, V Shasi Kiran AU - Reddy VSK AD - Division of Genetics & Plant Breeding, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Wadura, J&K, India. FAU - Rafiq, Ishrat AU - Rafiq I AD - Division of Genetics & Plant Breeding, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Wadura, J&K, India. FAU - Khan, Gazala H AU - Khan GH AD - Mountain Research Centre for Field Crops, Sher-e Kashmir University of Agricultural Sciences and Technology of Kashmir, Khudwani, J&K, India. FAU - Mohiddin, Fayaz AU - Mohiddin F AD - Mountain Research Centre for Field Crops, Sher-e Kashmir University of Agricultural Sciences and Technology of Kashmir, Khudwani, J&K, India. FAU - Zargar, Sajad M AU - Zargar SM AD - Division of Biotechnology, Sher-e Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, J&K, India. FAU - Hussain, Syed Zameer AU - Hussain SZ AD - Division of Food Science and Technology, Sher-e Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, J&K, India. FAU - Alie, Bashir A AU - Alie BA AD - Division of Agronomy, Sher-e Kashmir University of Agricultural Sciences and Technology of Kashmir, Wadura, J&K, India. FAU - Sofi, Najeeb-Ul-Rehman AU - Sofi NU AD - Mountain Research Centre for Field Crops, Sher-e Kashmir University of Agricultural Sciences and Technology of Kashmir, Khudwani, J&K, India. FAU - Shikari, Asif Bashir AU - Shikari AB AD - Division of Genetics & Plant Breeding, Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir, Wadura, J&K, India. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Plant Sci JT - Frontiers in plant science JID - 101568200 PMC - PMC13713480 OTO - NOTNLM OT - Mushk Budji OT - genes OT - genome OT - landrace OT - rice OT - sequencing COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The authors SZ, SH and AS declared that they were an editorial board member of Frontiers at the time of submission. This had no impact on the peer review process and the final decision. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:19 PHST- 2026/05/31 00:00 [received] PHST- 2026/07/24 00:00 [revised] PHST- 2026/08/03 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:19 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpls.2026.1896265 [doi] PST - epublish SO - Front Plant Sci. 2026 Oct 14;17:1896265. doi: 10.3389/fpls.2026.1896265. eCollection 2026. PMID- 42952841 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Prediction of residual stones after ESWL for ureteral calculi using deep learning-radiomics-based stone heterogeneity and body composition: an interaction analysis. PG - 1932507 LID - 10.3389/fmed.2026.1932507 [doi] LID - 1932507 AB - OBJECTIVE: To investigate the association between deep learning-radiomics-based stone heterogeneity, body composition, and residual stones after extracorporeal shock wave lithotripsy (ESWL) for ureteral calculi. METHODS: This retrospective study included 200 patients with ureteral stones who underwent ESWL between January 2024 and December 2025, comprising the development cohort. An additional 57 patients treated between January 2026 and June 2026 were included as the temporal testing cohort. Baseline clinical data and imaging data were collected. Deep learning (DL) and radiomics features of ureteral stones were extracted, and feature selection was performed to construct a DL-radiomics model (Radscore). TotalSegmentator was used to quantify visceral fat, skeletal muscle, and subcutaneous fat volumes within the T12-S4 region. The predictive performance of the clinical-body composition model, DL-radiomics model, and nomogram model was compared. Subgroup analyses were conducted to evaluate the stability of the nomogram model. The interaction between the Radscore and body composition in relation to residual stones was further explored using the optimal cutoff values derived from the training set. RESULTS: The development cohort was randomly divided into a training set and a validation set in a 7:3 ratio. Visceral fat and skeletal muscle volumes were included in the clinical-body composition model, yielding AUCs of 0.792 (0.706, 0.878), 0.821 (0.577, 1.000) and 0.827 (0.710, 0.943), respectively. The DL radiomics model achieved AUCs of 0.778 (0.679-0.876), 0.704 (0.363-1.000) and 0.742 (0.547-0.937). The nomogram model showed AUCs of 0.893 (0.835, 0.952), 0.824 (0.630, 1.000) and 0.898 (0.807, 0.989). Subgroup analyses of stone location, side of the stone, and perinephritis revealed no significant interaction in the nomogram model (P > 0.05). Calibration and decision curve analyses demonstrated good agreement and clinical utility of the nomogram model. In the interaction analysis between Radscore and body composition, visceral fat volume significantly attenuated the risk of residual stones associated with a high stone Radscore. CONCLUSION: Deep learning-radiomics-based stone heterogeneity demonstrates good predictive performance for residual stones after ESWL. Moreover, lower visceral fat volume significantly attenuates the impact of stone heterogeneity on residual stone formation. CI - (c) 2026 Wei, Fang, Zhang and Zhou. FAU - Wei, Shumin AU - Wei S AD - Medical Imaging Department, Xinghua People's Hospital Affiliated to Yangzhou University, Xinghua City, Jiangsu, China. FAU - Fang, Qingqing AU - Fang Q AD - Medical Imaging Department, Xinghua People's Hospital Affiliated to Yangzhou University, Xinghua City, Jiangsu, China. FAU - Zhang, Lin AU - Zhang L AD - Department of Urology, Xinghua People's Hospital Affiliated to Yangzhou University, Xinghua City, Jiangsu, China. FAU - Zhou, Liwei AU - Zhou L AD - Department of Urology, Xinghua People's Hospital Affiliated to Yangzhou University, Xinghua City, Jiangsu, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13713844 OTO - NOTNLM OT - body composition OT - deep learning OT - extracorporeal shock wave lithotripsy OT - interaction analysis OT - residual stone COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:19 PHST- 2026/07/09 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:19 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmed.2026.1932507 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 14;13:1932507. doi: 10.3389/fmed.2026.1932507. eCollection 2026. PMID- 42952817 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-1078 (Print) IS - 1664-1078 (Electronic) IS - 1664-1078 (Linking) VI - 17 DP - 2026 TI - Adaptation to university life, fear of loneliness, and depression in first-year students at a private university in the Peruvian Amazon: a structural equation model. PG - 1935089 LID - 10.3389/fpsyg.2026.1935089 [doi] LID - 1935089 AB - INTRODUCTION: The transition to university is a period of heightened psychological vulnerability. This study aimed to examine the association between adaptation to university life, fear of loneliness, and depression in Peruvian first-year university students. METHODS: A quantitative cross-sectional study was conducted with 345 participants selected through non-probability convenience sampling at a single private university in the Peruvian Amazon. The Student Adaptation to College Questionnaire, the Brief Scale of Fear of Loneliness, and the Patient Health Questionnaire-9 were administered, and data were analyzed using structural equation modeling (SEM). RESULTS: The proposed model showed an adequate fit [chi(2) (516) = 1,478, p < 0.001, CFI = 0.940, RMSEA = 0.074, SRMR = 0.069]. Personal-emotional adjustment (beta = 0.49, p < 0.001) and social adjustment (beta = 0.29, p = 0.003) were positively associated with fear of loneliness, whereas academic adjustment (beta = -0.34, p < 0.001) and institutional adjustment (beta = -0.22, p = 0.001) were negatively associated; in turn, fear of loneliness was positively related to depression (beta = 0.81, p < 0.001). Significant gender differences were found in institutional adjustment [t (343) = 2.597, p = 0.010, d = 0.29] and personal-emotional adjustment [t (343) = 3.076, p = 0.002, d = 0.34], with women reporting higher levels than men. DISCUSSION: The findings position fear of loneliness as a key affective link between first-year students' adjustment to university and their depressive symptoms, suggesting that early detection and intervention programs focused on adjustment, implemented during the first year, could help mitigate depression in this population. CI - Copyright (c) 2026 Gebol-Checan, Ascorbe-Pena, Miguel-Marino, Tapullima-Mori, Cjuno and Mayta-Llanos. FAU - Gebol-Checan, Rosa AU - Gebol-Checan R AD - Escuela Profesional de Psicologia, Universidad Peruana Union, Tarapoto, Peru. FAU - Ascorbe-Pena, Gianella AU - Ascorbe-Pena G AD - Escuela Profesional de Psicologia, Universidad Peruana Union, Tarapoto, Peru. FAU - Miguel-Marino, Royer AU - Miguel-Marino R AD - Escuela Profesional de Psicologia, Universidad Peruana Union, Tarapoto, Peru. FAU - Tapullima-Mori, Calixto AU - Tapullima-Mori C AD - Escuela Profesional de Psicologia, Universidad Peruana Union, Tarapoto, Peru. FAU - Cjuno, Julio AU - Cjuno J AD - Unidad de Posgrado de Psicologia, Universidad Peruana Union, Lima, Peru. FAU - Mayta-Llanos, Jhordan AU - Mayta-Llanos J AD - Escuela Profesional de Psicologia, Universidad Peruana Union, Tarapoto, Peru. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Psychol JT - Frontiers in psychology JID - 101550902 PMC - PMC13713792 OTO - NOTNLM OT - Peruvian students OT - depression OT - fear of loneliness OT - first-year students OT - university adaptation COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:18 PHST- 2026/07/11 00:00 [received] PHST- 2026/09/08 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:18 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpsyg.2026.1935089 [doi] PST - epublish SO - Front Psychol. 2026 Oct 14;17:1935089. doi: 10.3389/fpsyg.2026.1935089. eCollection 2026. PMID- 42952813 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-2360 (Print) IS - 2296-2360 (Electronic) IS - 2296-2360 (Linking) VI - 14 DP - 2026 TI - Totally single-incision laparoscopic choledochal cyst excision with intracorporeal hand-sewn Roux-en-Y reconstruction in children: clinical outcomes and learning curve. PG - 1951655 LID - 10.3389/fped.2026.1951655 [doi] LID - 1951655 AB - OBJECTIVE: To evaluate the feasibility, perioperative outcomes, and learning curve of totally single-incision laparoscopic choledochal cyst excision with intracorporeal hand-sewn Roux-en-Y reconstruction in children. METHODS: We retrospectively reviewed children who underwent laparoscopic choledochal cyst excision between January 2018 and December 2024. Thirty-five children underwent totally single-incision laparoscopic intracorporeal hand-sewn reconstruction (TSILC), and 40 underwent conventional single-incision laparoscopic-assisted reconstruction (SILC). Baseline characteristics, operative outcomes, recovery, complications, and follow-up were compared. Multivariable linear regression with HC3 robust standard errors was used to adjust continuous outcomes for baseline differences. The TSILC learning curve was assessed using cumulative sum (CUSUM) analysis of jejunojejunostomy time. RESULTS: TSILC patients were older (29.86 +/- 12.99 vs. 19.60 +/- 13.62 months, P = 0.001), heavier (14.90 +/- 5.07 vs. 9.22 +/- 4.40 kg, P < 0.001), and had a different sex distribution (P = 0.030). After adjustment, total operative time did not differ significantly (adjusted beta=8.75 min, 95% CI -12.10 to 29.60; P = 0.411), whereas jejunojejunostomy time remained longer with TSILC (adjusted beta=12.02 min, 95% CI 3.02 to 21.02; P = 0.009). TSILC was associated with less blood loss, a shorter incision, and earlier first flatus and defecation (all adjusted P < 0.001); oral intake time did not differ significantly after adjustment (P = 0.568). Early complications occurred in 3/35 TSILC and 5/40 SILC patients. Thirty-three TSILC procedures were completed without an additional port or open conversion; one required an additional port and one required open conversion. Median follow-up was 40.0 months (IQR 21.5-59.5) for TSILC and 46.5 months (IQR 27.2-66.2) for SILC. The CUSUM curve peaked at case 17, suggesting relative stabilization after case 18. CONCLUSION: TSILC with intracorporeal hand-sewn Roux-en-Y reconstruction was technically feasible in selected children and showed a measurable learning curve. The findings suggest that TSILC may offer perioperative advantages in appropriately selected older and larger children. CI - (c) 2026 Luo, Qin, Tang and Zhuang. FAU - Luo, Miao AU - Luo M AD - Department of Pediatric General Surgery, Shanghai Children's Medical Center Guizhou Hospital, Shanghai Jiao Tong University School of Medicine, Guiyang, China. FAU - Qin, Zhijie AU - Qin Z AD - Department of Pediatric General Surgery, Shanghai Children's Medical Center Guizhou Hospital, Shanghai Jiao Tong University School of Medicine, Guiyang, China. FAU - Tang, Yingming AU - Tang Y AD - Department of Pediatric General Surgery, Shanghai Children's Medical Center Guizhou Hospital, Shanghai Jiao Tong University School of Medicine, Guiyang, China. FAU - Zhuang, Yize AU - Zhuang Y AD - Department of Pediatric General Surgery, Shanghai Children's Medical Center Guizhou Hospital, Shanghai Jiao Tong University School of Medicine, Guiyang, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Pediatr JT - Frontiers in pediatrics JID - 101615492 PMC - PMC13713800 OTO - NOTNLM OT - CUSUM analysis OT - Roux-en-Y reconstruction OT - children OT - choledochal cyst OT - single-incision laparoscopy COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:17 PHST- 2026/07/29 00:00 [received] PHST- 2026/08/21 00:00 [revised] PHST- 2026/09/14 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:17 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fped.2026.1951655 [doi] PST - epublish SO - Front Pediatr. 2026 Oct 14;14:1951655. doi: 10.3389/fped.2026.1951655. eCollection 2026. PMID- 42952812 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1662-5099 (Print) IS - 1662-5099 (Electronic) IS - 1662-5099 (Linking) VI - 19 DP - 2026 TI - Combining pharmacological and non-invasive brain stimulation strategies to promote remyelination in multiple sclerosis. PG - 1980126 LID - 10.3389/fnmol.2026.1980126 [doi] LID - 1980126 AB - Remyelination in multiple sclerosis (MS) fails for several, partly overlapping reasons. The differentiation of oligodendrocyte precursor cells into myelin-producing oligodendrocytes is impaired both by intrinsic factors and by the surrounding lesion environment, which becomes increasingly inflammatory and senescent with age. Pharmacological strategies acting on a single target to promote oligodendrocyte lineage progression have been successful in animal models but have yet to produce meaningful clinical benefit in people with MS (pwMS). In this perspective paper, we argue that simultaneously targeting several of the mechanisms underlying this failure is a more promising route to repairing myelin lesions in pwMS. We outline how non-invasive brain stimulation (NIBS) might be leveraged to shift the glial microenvironment towards a pro-regenerative, remyelinating state, and how this can be paired with targeted pharmacological agents that enhance oligodendrocyte differentiation. We also discuss how the expected effect of such a combination may depend on age and lesion stage. This framework may offer a rational route beyond the translational failures of single-target remyelination therapies. CI - Copyright (c) 2026 Scheinok, De Bundel, Vanmierlo and Van Schependom. FAU - Scheinok, Thomas Jonathan AU - Scheinok TJ AD - AIMS Lab, Center for Neurosciences (C4N), UZ Brussel, Vrije Universiteit Brussel, Brussels, Belgium. AD - Laboratory of Pharmaceutical Chemistry, Drug Analysis and Drug Information (FASC), Research Group Experimental Pharmacology (EFAR), Center for Neurosciences (C4N), Vrije Universiteit Brussel (VUB), Brussels, Belgium. AD - NEUR Research Group, Center for Neurosciences (C4N), Vrije Universiteit Brussel, Brussels, Belgium. FAU - De Bundel, Dimitri AU - De Bundel D AD - Laboratory of Pharmaceutical Chemistry, Drug Analysis and Drug Information (FASC), Research Group Experimental Pharmacology (EFAR), Center for Neurosciences (C4N), Vrije Universiteit Brussel (VUB), Brussels, Belgium. FAU - Vanmierlo, Tim AU - Vanmierlo T AD - Division of Translational Neuroscience, Department of Psychiatry and Neuropsychology, Mental Health and Neuroscience Research Institute (MHeNS), Maastricht University, Maastricht, Netherlands. AD - Department of Neuroscience, Biomedical Research Institute (BIOMED), Hasselt University, Diepenbeek, Belgium. FAU - Van Schependom, Jeroen AU - Van Schependom J AD - AIMS Lab, Center for Neurosciences (C4N), UZ Brussel, Vrije Universiteit Brussel, Brussels, Belgium. AD - Department of Electronics and Informatics (ETRO), Vrije Universiteit Brussel, Brussels, Belgium. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Mol Neurosci JT - Frontiers in molecular neuroscience JID - 101477914 PMC - PMC13713505 OTO - NOTNLM OT - combination therapy OT - microglia OT - multiple sclerosis OT - neuroinflammation OT - non-invasive brain stimulation OT - oligodendrocyte precursor cells OT - remyelination COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:17 PHST- 2026/08/27 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:17 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fnmol.2026.1980126 [doi] PST - epublish SO - Front Mol Neurosci. 2026 Oct 14;19:1980126. doi: 10.3389/fnmol.2026.1980126. eCollection 2026. PMID- 42952806 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-2360 (Print) IS - 2296-2360 (Electronic) IS - 2296-2360 (Linking) VI - 14 DP - 2026 TI - Multicenter cohort of necrotizing enterocolitis in Latin America: evaluation of risk factors and clinical outcomes in Chilean Hospitals. PG - 1877057 LID - 10.3389/fped.2026.1877057 [doi] LID - 1877057 AB - BACKGROUND: Necrotizing enterocolitis (NEC) remains a leading cause of neonatal morbidity. While risk factors are well documented in high-income countries, data from Latin American cohorts remain limited. OBJECTIVE: We aimed to identify local predictors and clinical outcomes associated with NEC in two Chilean NICUs. METHODS: A prospective multicenter cohort study (2017-2018) included newborns /= IIA) was verified by a multidisciplinary team. Clinical and laboratory variables were analyzed using bivariate and multivariable penalized models to identify predictors of NEC and mortality. RESULTS: Among 177 newborns (mean GA 29.1 +/- 3.0 weeks; BW 1,252 +/- 448 g), NEC incidence was 7.9% (n = 14). In bivariate analysis, invasive mechanical ventilation (RR = 4.58; p = 0.02) and indomethacin (RR = 3.13; p = 0.04) were associated with NEC. In multivariable models, formula feeding was the most consistent predictor (OR = 2.69 vs. exclusive breast milk), though not statistically significant. Half of NEC cases (50%) were Bell stage IIIB. Surgery was required in 38.5% of cases (all stage IIIB; p = 0.009). Case fatality among NEC patients was 40%, associated with Bell stage IIIB (p = 0.048) and systemic shock (p = 0.048). CONCLUSION: Formula feeding was a consistent, though non-significant, predictor of NEC in multivariable analysis; the limited number of NEC cases (n = 14) precludes definitive causal inference. Exclusive breastfeeding remained the most protective feeding strategy, consistent with prior literature. Disease outcomes were driven by progression to Bell stage IIIB, and early monitoring of inflammatory and metabolic biomarkers may help identify infants at risk of progression to surgical NEC. CI - (c) 2026 Sandoval C., Delpiano M., Pantoja H., Aravena U., Smith R., Perez A. and Espinoza E.. FAU - Sandoval C, Alejandra AU - Sandoval C A AD - Hospital Carlos Van Buren, Valparaiso, Chile. AD - Departamento de Pediatria, Universidad de Valparaiso, Valparaiso, Chile. FAU - Delpiano M, Luis AU - Delpiano M L AD - Hospital Clinico San Borja-Arriaran, Santiago, Chile. AD - Facultad de Medicina, Universidad de Chile, Santiago, Chile. FAU - Pantoja H, Miguel Angel AU - Pantoja H MA AD - Hospital Clinico San Borja-Arriaran, Santiago, Chile. AD - Facultad de Medicina, Universidad de Chile, Santiago, Chile. AD - Servicio de Neonatologia, Clinica Alemana, Santiago, Chile. FAU - Aravena U, Marta AU - Aravena U M AD - Hospital Sotero del Rio, Santiago, Chile. AD - Servicio de Neonatologia, Hospital Sotero del Rio, Santiago, Chile. FAU - Smith R, Jeanne AU - Smith R J AD - Hospital Sotero del Rio, Santiago, Chile. AD - Servicio de Neonatologia, Hospital Sotero del Rio, Santiago, Chile. FAU - Perez A, Hector AU - Perez A H AD - Hospital Sotero del Rio, Santiago, Chile. AD - Servicio de Neonatologia, Hospital Sotero del Rio, Santiago, Chile. FAU - Espinoza E, Sebastian AU - Espinoza E S AD - Instituto de Tecnologia Para la Innovacion en Salud y Bienestar (ITiSB)-UNAB, Vina del Mar, Chile. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Pediatr JT - Frontiers in pediatrics JID - 101615492 PMC - PMC13713484 OTO - NOTNLM OT - mortality OT - necrotizing enterocolitis OT - neonatal intensive care OT - pneumatosis intestinalis OT - preterm infant COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:17 PHST- 2026/05/09 00:00 [received] PHST- 2026/08/23 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:17 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fped.2026.1877057 [doi] PST - epublish SO - Front Pediatr. 2026 Oct 14;14:1877057. doi: 10.3389/fped.2026.1877057. eCollection 2026. PMID- 42952798 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-861X (Print) IS - 2296-861X (Electronic) IS - 2296-861X (Linking) VI - 13 DP - 2026 TI - D-allulose attenuates dyslipidemia, oxidative stress, and inflammation in high-fat-diet-induced metabolic dysfunction-associated steatotic liver disease in rats. PG - 1982241 LID - 10.3389/fnut.2026.1982241 [doi] LID - 1982241 AB - BACKGROUND: Metabolic dysfunction-associated steatotic liver disease (MASLD) is a diet-related metabolic disorder characterized by hepatic lipid accumulation, dyslipidemia, insulin resistance, oxidative stress, and chronic inflammation, highlighting the need for safe nutritional interventions. AIM: This study evaluated the antihyperlipidemic, antioxidant, and anti-inflammatory effects of D-allulose, alone or in combination with dorsomorphin, in a high-fat diet (HFD)-induced rat model of Metabolic dysfunction-associated steatotic liver disease (MASLD). METHODS: Forty-eight male Wistar rats were allocated to a control diet group, an HFD group, or an HFD group treated orally with D-allulose (0.3 or 0.6 g/kg) with or without dorsomorphin (0.2 mg/kg) orally for 12 weeks. Omnibus 𝑝-values for the 22 biochemical endpoints were subjected to a retrospective Benjamini-Hochberg false-discovery-rate sensitivity analysis. RESULTS: HFD feeding induced weight gain, dyslipidemia, hepatic injury, oxidative stress, inflammation, and hyperinsulinemia. D-allulose dose-dependently ameliorated these disturbances, reducing atherogenic lipids, lipid peroxidation, inflammatory cytokines, and insulin while improving antioxidant status. Notably, TG decreased from 164.51 mg dL(-1) in HFD rats to 113.52 and 99.43 mg dL(-1) with low- and high-dose D-allulose, respectively, while LDL-C decreased from 86.45 to 53.19 and 49.24 mg dL(-1); HDL-C remained unchanged. D-allulose also attenuated HFD-induced hepatic steatosis, degeneration, necrosis, inflammation, and early fibrosis, and this histological protection was maintained or enhanced by dorsomorphin co-administration. A retrospective FDR sensitivity analysis supported the principal lipid, antioxidant, inflammatory, and insulin findings, whereas the ALT and FBG findings did not remain significant and were considered exploratory. CONCLUSION: The combination groups generally matched or outperformed D-allulose alone across several biochemical and histological endpoints. However, because dorsomorphin is a non-selective AMPK/BMP inhibitor and AMPK activity was not directly measured, these findings do not establish AMPK's mechanistic involvement. Overall, D-allulose shows promising protective impacts as a bioactive ingredient for obesity-related metabolic disorders. CI - Copyright (c) 2026 Almutairi, Yagoub and Barakat. FAU - Almutairi, Sami O AU - Almutairi SO AD - Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia. FAU - Yagoub, Abu ElGasim A AU - Yagoub AEA AD - Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, Saudi Arabia. FAU - Barakat, Hassan AU - Barakat H AD - Department of Food Science and Human Nutrition, College of Agriculture and Food, Qassim University, Buraydah, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Nutr JT - Frontiers in nutrition JID - 101642264 PMC - PMC13713509 OTO - NOTNLM OT - D-allulose OT - MASLD OT - anti-inflammatory OT - antioxidant activity OT - food supply OT - lipid metabolism OT - rat model COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:16 PHST- 2026/08/29 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:16 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fnut.2026.1982241 [doi] PST - epublish SO - Front Nutr. 2026 Oct 14;13:1982241. doi: 10.3389/fnut.2026.1982241. eCollection 2026. PMID- 42952793 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-2565 (Electronic) IS - 2296-2565 (Linking) VI - 14 DP - 2026 TI - Prediction of HIV incidence in Xinjiang Uygur autonomous region, China: a time series study. PG - 1920865 LID - 10.3389/fpubh.2026.1920865 [doi] LID - 1920865 AB - OBJECTIVE: In China, Xinjiang Uygur Autonomous Region has a high incidence of HIV, and the situation regarding its prevention and control remains challenging. This study aimed to compare the predictive performance of three time series models, SARIMAX (Seasonal Autoregressive Integrated Moving Average with Exogenous Regressors), Prophet with exogenous variables, and random forest, using rolling validation, and to provide references for HIV prevention and control in Xinjiang. METHODS: We collected 168 months of HIV incidence data in Xinjiang from 2012 to 2025. SARIMAX, Prophet, and random forest were developed with COVID-19 as an exogenous intervention variable. Prophet was tuned via grid search (optimal changepoint.prior.scale=0.1, seasonality.prior.scale=1); random forest was optimized with mtry=3 and 12-month lags selected by PACF. Rolling validation used RMSE, MAE, MAPE, sMAPE, and MASE with Bootstrap 95% CI. SARIMAX failed to converge in the first four windows; therefore, models were compared on windows 5-9 (2021-2025). RESULTS: The optimal SARIMAX model was SARIMAX(0, 1, 4)(1, 0, 0)(1) with an exogenous COVID-19 intervention variable; its residuals passed the Ljung-Box test (Q = 8.8, p = 0.7199). The Prophet model incorporated seasonal and periodic components and included COVID-19 as an exogenous variable; Bayesian MCMC inference gave a posterior mean of -0.1064. The random forest model used 12 lagged values, month, and COVID-19 as features, achieving a permutation importance (%IncMSE) of 4.31% for the COVID-19 variable, ranking 10th among 14 features. SHapley Additive exPlanations (SHAP) analysis further showed that COVID-19 ranked 14th (last) with a mean absolute SHAP value of 0.00135. Within windows 5-9 (2021-2025), random forest had the lowest RMSE (0.2211), MAE (0.1722), and MAPE (85.83%); (sMAPE 41.11%, MASE 0.8745). However, Diebold-Mariano tests with Bonferroni correction showed no significant differences among models (all p > 0.0167), with small RMSE differences (0.0047-0.0225). CONCLUSIONS: All three models are applicable. Although random forest had the lowest numerical errors, it was statistically indistinguishable from the other models; therefore, model selection should consider interpretability, data-updating frequency, and resource constraints rather than marginal accuracy differences. These findings support HIV surveillance and resource allocation in Xinjiang. CI - Copyright (c) 2026 Zhang and Zhao. FAU - Zhang, Tao AU - Zhang T AD - Department of Hospital Administration Office, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China. FAU - Zhao, Daren AU - Zhao D AD - Faculty of Social Sciences and Humanities, Mahidol University, Salaya, Nakhon Pathom Province, Thailand. AD - Department of Quality Control Management, Sichuan Provincial Orthopedics Hospital, Chengdu, Sichuan, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Public Health JT - Frontiers in public health JID - 101616579 SB - IM MH - China/epidemiology MH - Humans MH - *HIV Infections/epidemiology MH - Incidence MH - Random Forest MH - Prediction Algorithms MH - COVID-19/epidemiology MH - Forecasting MH - Models, Statistical PMC - PMC13713725 OTO - NOTNLM OT - China OT - HIV OT - SARIMAX OT - exogenous variable OT - prophet model OT - random forest OT - time series COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:15 PHST- 2026/06/26 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:15 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpubh.2026.1920865 [doi] PST - epublish SO - Front Public Health. 2026 Oct 14;14:1920865. doi: 10.3389/fpubh.2026.1920865. eCollection 2026. PMID- 42952785 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Endoscopic ultrasound service readiness across a provincial endoscopy network in Hubei, China: a cross-sectional institutional survey. PG - 1937464 LID - 10.3389/fmed.2026.1937464 [doi] LID - 1937464 AB - BACKGROUND: Endoscopic ultrasound (EUS) is essential for pancreatobiliary evaluation, subepithelial-lesion assessment, tumor staging, and tissue acquisition. We evaluated EUS service availability, institutional resources, and quality-indicator reporting across a provincial endoscopy network in Hubei, China. METHODS: We conducted a cross-sectional survey of 99 digestive endoscopy institutions from January to March 2026. Institutions were classified by administrative level and four mutually exclusive capability tiers: no EUS, miniprobe-only EUS, diagnostic radial/linear EUS without EUS-FNA, and EUS-guided fine-needle aspiration (EUS-FNA). Analyses included descriptive statistics, Wilson 95% confidence intervals, and exploratory univariable associations. RESULTS: Any EUS service was reported by 33/99 institutions (33.3%). Of these, 16 provided miniprobe-only EUS, 6 provided diagnostic radial/linear EUS without EUS-FNA, and 11 provided EUS-FNA. The diagnostic radial/linear and EUS-FNA tiers together comprised 17 institutions with radial/linear EUS capability, all of which were city-level hospitals. Median annual endoscopy volume was 1,518.5 (IQR, 309.3-4,000) in the no-EUS tier and 21,293 (IQR, 10,032-29,810.5) in the EUS-FNA tier; corresponding endoscopist counts were 4 (IQR, 2-6) and 14 (IQR, 11-19.5). The eight highest-volume institutions accounted for 81.9% of reported EUS activity. EUS completion-rate reporting was present in 30/33 EUS providers (90.9%), and EUS-FNA pathological-yield reporting in 9/11 EUS-FNA providers (81.8%). CONCLUSIONS: EUS services in the participating network were concentrated in city-level hospitals, with higher median annual endoscopy volume and endoscopist staffing in the higher capability tiers. These findings describe service provision within the surveyed institutions and identify questions for future evaluation of referral, training, and quality monitoring. CI - (c) 2026 Zhang, Li, Huang and Yu. FAU - Zhang, Jun AU - Zhang J AD - Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, China. AD - Hubei Provincial Clinical Research Center for Digestive Disease Minimally Invasive Incision, Renmin Hospital of Wuhan University, Wuhan, China. AD - Engineering Research Center for Artificial Intelligence Endoscopy Interventional Treatment of Hubei Province, Renmin Hospital of Wuhan University, Wuhan, China. FAU - Li, Yanxia AU - Li Y AD - Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, China. AD - Hubei Provincial Clinical Research Center for Digestive Disease Minimally Invasive Incision, Renmin Hospital of Wuhan University, Wuhan, China. AD - Engineering Research Center for Artificial Intelligence Endoscopy Interventional Treatment of Hubei Province, Renmin Hospital of Wuhan University, Wuhan, China. FAU - Huang, Xu AU - Huang X AD - Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, China. AD - Hubei Provincial Clinical Research Center for Digestive Disease Minimally Invasive Incision, Renmin Hospital of Wuhan University, Wuhan, China. AD - Engineering Research Center for Artificial Intelligence Endoscopy Interventional Treatment of Hubei Province, Renmin Hospital of Wuhan University, Wuhan, China. FAU - Yu, Honggang AU - Yu H AD - Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, China. AD - Hubei Provincial Clinical Research Center for Digestive Disease Minimally Invasive Incision, Renmin Hospital of Wuhan University, Wuhan, China. AD - Engineering Research Center for Artificial Intelligence Endoscopy Interventional Treatment of Hubei Province, Renmin Hospital of Wuhan University, Wuhan, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13713730 OTO - NOTNLM OT - EUS-guided fine-needle aspiration OT - endoscopic ultrasound OT - institutional survey OT - quality monitoring OT - service readiness COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:14 PHST- 2026/07/14 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:14 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmed.2026.1937464 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 14;13:1937464. doi: 10.3389/fmed.2026.1937464. eCollection 2026. PMID- 42952783 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Development and multi-database validation of machine learning models for 28-day mortality prediction in ICU patients with COPD and pulmonary hypertension. PG - 1959885 LID - 10.3389/fmed.2026.1959885 [doi] LID - 1959885 AB - BACKGROUND: Early mortality prediction in critically ill patients with chronic obstructive pulmonary disease (COPD) coexisting pulmonary hypertension (PH) remains challenging, particularly because prediction models developed in intensive care unit (ICU) database may not perform consistently across other healthcare systems. METHODS: This retrospective multicenter study employed two large-scale databases to establish and validate ML models for predicting early mortality in ICU patients with COPD and PH. The MIMIC-IV database served as the model-development cohort (2555 patients), with external validation conducted on eICU CRD (292 patients) database. Six ML algorithms were adopted: Random Forest (RF), Extreme Gradient Boosting (XGBoost), Logistic Regression (LR), Light Gradient Boosting Machine (LightGBM), Support Vector Machines (SVM), and Artificial Neural Network (ANN).The baseline variables were screened to identify potential predictors via least absolute shrinkage and selection operator (Lasso) regression. Model interpretability was evaluated via SHapley Additive exPlanations (SHAP) analysis. RESULTS: The overall cohort consisted of 2,847 ICU patients with COPD and PH. Twelve consistently crucial clinical features were identified, including acute kidney injury, pneumonia, age, weight, heart rate, red blood cell distribution width (RDW), white blood cell (WBC), anion gap, chloride, creatinine, base excess, potential of hydrogen (pH). The Random Forest algorithm achieved the optimal performance, with a training Area Under Curve (AUC) of 0.938, internal validation AUC of 0.748, and eICU CRD external validation AUC of 0.800. SHAP analysis indicated that acute kidney injury was the most influential predictor, followed by base excess and RDW. Additionally, a web tool was developed in this study to facilitate the application of early death risk prediction in clinical practice. CONCLUSIONS: This study is the multi-database validation of ML models for early mortality prediction in ICU patients with COPD and PH. The Random Forest model integrated with SHAP interpretability offers a reliable tool for clinical decision-making support, with consistent validity verified across two databases, including our internal validation cohort. This work provides a decision support tool to assist in identifying high-risk individuals within this complex comorbid population, and establishes a foundation for future studies with a more dynamic and personalized prognostic approach. CI - (c) 2026 Xia, Yang and Yang. FAU - Xia, Yuwei AU - Xia Y AD - The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, Zhejiang, China. FAU - Yang, Yuxuan AU - Yang Y AD - Pharmacy 4 Less Pty Ltd, Sydney, NSW, Australia. FAU - Yang, Junchao AU - Yang J AD - The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, Zhejiang, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13713754 OTO - NOTNLM OT - SHAP analysis OT - chronic obstructive pulmonary disease OT - machine learning OT - mortality prediction OT - multi-database validation OT - pulmonary hypertension COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:14 PHST- 2026/08/06 00:00 [received] PHST- 2026/09/12 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:14 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmed.2026.1959885 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 14;13:1959885. doi: 10.3389/fmed.2026.1959885. eCollection 2026. PMID- 42952779 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Risk factors for poor prognosis in neonatal sepsis: a systematic review and meta-analysis. PG - 1979094 LID - 10.3389/fmed.2026.1979094 [doi] LID - 1979094 AB - OBJECTIVE: To synthesize previous research findings through systematic review and meta-analysis and to evaluate risk factors associated with poor prognosis in neonatal sepsis, thereby providing additional evidence for early clinical risk assessment. METHODS: According to the study objectives, population, and study design, the inclusion criteria, exclusion criteria, and detailed search strategy were established. Eight databases were searched, including PubMed, Embase, Web of Science, the Cochrane Library, China National Knowledge Infrastructure (CNKI), Wanfang Data, VIP Chinese Science and Technology Journals Database, and the Chinese Biomedical Literature Database (CBM), from database inception to January 21, 2026. The specific coverage periods were as follows: PubMed (1946 to January 21, 2026), Embase (1947 to January 21, 2026), the Cochrane Library (1992 to January 21, 2026), Web of Science (1900 to January 21, 2026), CNKI (1915 to January 21, 2026), VIP Chinese Science and Technology Journals Database (1989 to January 21, 2026), CBM (1994 to January 21, 2026), and Wanfang Data (1998 to January 21, 2026). Studies meeting the inclusion criteria were selected. Study selection and data extraction were independently performed by two reviewers. Methodological quality was assessed using the Newcastle-Ottawa Scale, and statistical analyses were conducted using RevMan 5.4. RESULTS: A total of 26 studies were included. The main independent risk factors for poor prognosis in neonatal sepsis were as follows: 1 in circle elevated interleukin-6 [OR = 1.97, p < 0.001]; 2 in circle elevated procalcitonin [OR = 1.59, p < 0.001]; 3 in circle thrombocytopenia [OR = 2.47, p = 0.020]; 4 in circle elevated C-reactive protein [OR = 2.04, p = 0.003]; ⑤ leukopenia [OR = 2.90, p = 0.001]; ⑥ elevated lactate [OR = 2.18, p = 0.005]; ⑦ Gram-negative bacterial infection [OR = 5.37, p = 0.002]; and ⑧ decreased albumin [OR = 5.77, p = 0.001]. CONCLUSION: Elevated inflammatory biomarkers, hematologic abnormalities, hyperlactatemia, hypoalbuminemia, and Gram-negative bacterial infection are important indicators of poor prognosis in neonatal sepsis. Early recognition of these factors and timely intervention may improve clinical outcomes. SYSTEMATIC REVIEW REGISTRATION: https://www.crd.york.ac.uk/PROSPERO/view/CRD420261290404, CRD420261290404. CI - (c) 2026 Yang, Han, Wang and Lu. FAU - Yang, Dejuan AU - Yang D AD - Department of Laboratory Medicine, Qinghai Provincial Women and Children's Hospital, Xining, Qinghai, China. FAU - Han, Yan AU - Han Y AD - Neonatal Mother-Infant Rooming-in Unit, Qinghai Provincial Women and Children's Hospital, Xining, Qinghai, China. FAU - Wang, Liping AU - Wang L AD - Department of Laboratory Medicine, Qinghai Provincial Women and Children's Hospital, Xining, Qinghai, China. FAU - Lu, Wenhua AU - Lu W AD - Department of Neonatology, Qinghai Provincial Women and Children's Hospital, Xining, Qinghai, China. LA - eng PT - Journal Article PT - Systematic Review DEP - 20261014 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13713741 OTO - NOTNLM OT - meta-analysis OT - neonate OT - poor prognosis OT - risk factors OT - sepsis OT - systematic review COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:14 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:14 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmed.2026.1979094 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 14;13:1979094. doi: 10.3389/fmed.2026.1979094. eCollection 2026. PMID- 42952754 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-2392 (Print) IS - 1664-2392 (Electronic) IS - 1664-2392 (Linking) VI - 17 DP - 2026 TI - Network and pairwise meta-analysis of the effects of novel hypoglycemic agents on cardiorenal outcomes in patients with type 2 diabetes and heart failure. PG - 1955431 LID - 10.3389/fendo.2026.1955431 [doi] LID - 1955431 AB - OBJECTIVE: This study aims to evaluate the cardiovascular and renal benefits of novel hypoglycemic agents, including glucagon-like peptide-1 receptor agonists (GLP-1RAs), dipeptidyl peptidase-4 inhibitors (DPP-4is), sodium-glucose cotransporter-2 inhibitors (SGLT-2is), and glucose-dependent insulinotropic polypeptide/glucagon-like peptide-1 dual receptor agonists (GIP/GLP-1RAs) in patients with type 2 diabetes mellitus (T2DM) and heart failure (HF). METHODS: Systematic literature searches were conducted in MEDLINE, Embase, ClinicalTrials.gov, and Cochrane Central Register of Controlled Trials (CENTRAL) from inception to 30 April 2026. Randomized controlled trials (RCTs) for cardiovascular outcomes (CVOTs) or post hoc analyses of CVOTs comparing SGLT-2is, DPP-4is, GLP-1RAs, or GIP/GLP-1RAs in patients with T2DM and HF were selected. Network meta-analysis was used for indirect comparisons, with results presented as hazard ratios (HRs) and 95% confidence intervals (CIs). RESULTS: A total of 10 RCTs and two post hoc analyses of CVOTs involving 13,886 patients were included. Compared with placebo, SGLT-2is (HR [95% CI]: 0.75 [0.68, 0.82]) and GLP-1RAs (HR [95% CI]: 0.77 [0.61, 0.97]) both significantly reduced the risk of HF-related composite endpoints in patients with T2DM and HF. No statistically significant difference was observed between SGLT-2is and GLP-1RAs (HR [95% CI]: 0.98 [0.76, 1.25]). SGLT-2is also reduced the risk of HF worsening (HR [95% CI]: 0.72 [0.53, 0.96]), HF hospitalization (HR [95% CI]: 0.74 [0.66, 0.83]), and cardiovascular (CV) death (HR [95% CI]: 0.87 [0.77, 0.98]). For all-cause death, SGLT-2is showed a trend toward reduced risk compared to placebo, but this difference did not reach statistical significance (HR [95% CI]: 0.83 [0.67, 1.02]). For the renal composite outcome, SGLT-2is did not demonstrate a statistically significant benefit (HR [95% CI]: 0.76 [0.51, 1.14]). CONCLUSION: Optimal clinical management of T2DM requires a comprehensive assessment of cardiovascular and renal comorbidities. This network meta-analysis indicates that SGLT-2is significantly reduce the risk of HF-related composite endpoints and CV death in T2DM patients with HF, although their renoprotective effect does not reach statistical significance. GLP-1RAs also demonstrate beneficial effects on HF-related composite endpoints. These findings suggest that both SGLT-2is and GLP-1RAs are preferred glycemic control strategies for improving cardiovascular outcomes in T2DM patients with HF, while further evidence is needed to confirm their definitive renal benefits. SYSTEMATIC REVIEW REGISTRATION: https://www.crd.york.ac.uk/PROSPERO/view/CRD42026142123, identifier: CRD420261421235. CI - Copyright (c) 2026 Ye, Wu, Li, Huang and Wang. FAU - Ye, Xuejiao AU - Ye X AD - China Academy of Chinese Medical Sciences, Guang'anmen Hospital, Beijing, China. FAU - Wu, Qian AU - Wu Q AD - China Academy of Chinese Medical Sciences, Guang'anmen Hospital, Beijing, China. FAU - Li, Leyang AU - Li L AD - China Academy of Chinese Medical Sciences, Guang'anmen Hospital, Beijing, China. FAU - Huang, Mingyu AU - Huang M AD - China Academy of Chinese Medical Sciences, Guang'anmen Hospital, Beijing, China. FAU - Wang, Shihan AU - Wang S AD - China Academy of Chinese Medical Sciences, Guang'anmen Hospital, Beijing, China. LA - eng PT - Journal Article PT - Network Meta-Analysis PT - Systematic Review DEP - 20261014 PL - Switzerland TA - Front Endocrinol (Lausanne) JT - Frontiers in endocrinology JID - 101555782 RN - 0 (Hypoglycemic Agents) RN - 0 (Sodium-Glucose Transporter 2 Inhibitors) RN - 0 (Dipeptidyl-Peptidase IV Inhibitors) RN - 0 (Glucagon-Like Peptide-1 Receptor Agonists) SB - IM MH - Humans MH - *Diabetes Mellitus, Type 2/drug therapy/complications MH - *Heart Failure/drug therapy/complications MH - *Hypoglycemic Agents/therapeutic use MH - Sodium-Glucose Transporter 2 Inhibitors/therapeutic use MH - Dipeptidyl-Peptidase IV Inhibitors/therapeutic use MH - Randomized Controlled Trials as Topic MH - Glucagon-Like Peptide-1 Receptor Agonists PMC - PMC13713430 OTO - NOTNLM OT - dipeptidyl peptidase-4 inhibitor OT - glucagon-like peptide-1 receptor agonist OT - glucose-dependent insulinotropic polypeptide/glucagon-like peptide-1 dual receptor agonist OT - heart failure OT - sodium-glucose cotransporter-2 inhibitor OT - type 2 diabetes mellitus COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:12 PHST- 2026/08/01 00:00 [received] PHST- 2026/09/11 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:12 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fendo.2026.1955431 [doi] PST - epublish SO - Front Endocrinol (Lausanne). 2026 Oct 14;17:1955431. doi: 10.3389/fendo.2026.1955431. eCollection 2026. PMID- 42952753 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-2565 (Electronic) IS - 2296-2565 (Linking) VI - 14 DP - 2026 TI - Sport participation and gambling-related problems in adolescents: findings from a large school-based study. PG - 1931143 LID - 10.3389/fpubh.2026.1931143 [doi] LID - 1931143 AB - BACKGROUND: Adolescent gambling is increasingly shaped by digital access, sports betting, and gambling-like mechanisms embedded in everyday youth culture. This study examined gambling-related problems among high school students and their associations with sociodemographic, family, mental health-related, and behavioral characteristics, with particular attention to sport participation. METHODS: A school-based cross-sectional study was conducted among 2,379 high school students in Novi Sad, Serbia, between 2024 and 2026. Participants completed a structured sociodemographic and behavioral questionnaire and the Serbian version of the Gambling Disorder Identification Test. Students were classified as non-gambling, recreational gambling, or having gambling-related problems. Associations between gambling categories and selected characteristics were examined using omnibus chi-square tests, Cramer's V, and post-hoc pairwise comparisons. RESULTS: Overall, 1,969 students (82.8%) were classified as non-gambling, 314 (13.2%) as recreational gambling, and 96 (4.0%) as having gambling-related problems. Gambling category was significantly associated with male sex, lower school achievement, living arrangement, parental marital status, sport participation, previous psychiatric treatment, family psychiatric history, smoking, alcohol use, and psychoactive substance use. In the univariate analyses, sport participation increased from 64.0% among non-gambling students to 75.2% among recreational gamblers and 78.1% among students with gambling-related problems. Post-hoc comparisons showed that sport participation differentiated non-gambling students from both gambling-involved groups, but not recreational gambling from gambling-related problems. However, after adjustment for sex and other relevant behavioral and mental health-related characteristics, sport participation was no longer independently associated with gambling-related problems. Male sex, previous psychiatric treatment, smoking, alcohol use, and psychoactive substance use remained the strongest independent correlates. CONCLUSION: Gambling-related problems in adolescents were associated with a broader pattern of behavioral and mental health-related factors. Although sport participation was associated with gambling involvement in unadjusted analyses, this association did not remain significant after adjustment for sex and other relevant characteristics. These findings support understanding adolescent gambling within a broader risk-behavior and public health framework, with sport representing a relevant context for prevention rather than an independent risk factor. CI - Copyright (c) 2026 Ratkovic, Knezevic, Dickov, Ivanovic-Kovacevic, Savic, Ivetic, Bjelan and Comic. FAU - Ratkovic, Dragana AU - Ratkovic D AD - University of Novi Sad, Faculty of Medicine Novi Sad, Department of Psychiatry and Psychological Medicine, Novi Sad, Republic of Serbia. FAU - Knezevic, Vladimir AU - Knezevic V AD - University of Novi Sad, Faculty of Medicine Novi Sad, Department of Psychiatry and Psychological Medicine, Novi Sad, Republic of Serbia. FAU - Dickov, Aleksandra AU - Dickov A AD - University of Novi Sad, Faculty of Medicine Novi Sad, Department of Psychiatry and Psychological Medicine, Novi Sad, Republic of Serbia. FAU - Ivanovic-Kovacevic, Svetlana AU - Ivanovic-Kovacevic S AD - University of Novi Sad, Faculty of Medicine Novi Sad, Department of Psychiatry and Psychological Medicine, Novi Sad, Republic of Serbia. FAU - Savic, Predrag AU - Savic P AD - University of Novi Sad, Faculty of Medicine Novi Sad, Department of Psychiatry and Psychological Medicine, Novi Sad, Republic of Serbia. FAU - Ivetic, Olga AU - Ivetic O AD - University of Novi Sad, Faculty of Medicine Novi Sad, Department of Psychiatry and Psychological Medicine, Novi Sad, Republic of Serbia. FAU - Bjelan, Sanja AU - Bjelan S AD - University of Novi Sad, Faculty of Medicine Novi Sad, Department of Psychiatry and Psychological Medicine, Novi Sad, Republic of Serbia. FAU - Comic, Masa AU - Comic M AD - University of Novi Sad, Faculty of Medicine Novi Sad, Department of Psychiatry and Psychological Medicine, Novi Sad, Republic of Serbia. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Public Health JT - Frontiers in public health JID - 101616579 SB - IM MH - Humans MH - *Gambling/epidemiology/psychology MH - Male MH - Adolescent MH - Female MH - *Sports/statistics & numerical data/psychology MH - Cross-Sectional Studies MH - *Students/psychology/statistics & numerical data MH - Serbia/epidemiology MH - Schools MH - *Adolescent Behavior/psychology MH - Surveys and Questionnaires MH - Risk Factors PMC - PMC13713745 OTO - NOTNLM OT - adolescent gambling OT - behavioral addiction OT - gambling-related problems OT - public health OT - school-based study OT - sport participation OT - sports betting COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:12 PHST- 2026/07/07 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:12 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpubh.2026.1931143 [doi] PST - epublish SO - Front Public Health. 2026 Oct 14;14:1931143. doi: 10.3389/fpubh.2026.1931143. eCollection 2026. PMID- 42952745 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-4185 (Print) IS - 2296-4185 (Electronic) IS - 2296-4185 (Linking) VI - 14 DP - 2026 TI - Surface engineering strategies for PET-Based ligament scaffolds in anterior cruciate ligament reconstruction: a systematic review of mechanical and biological performances. PG - 1916072 LID - 10.3389/fbioe.2026.1916072 [doi] LID - 1916072 AB - BACKGROUND/OBJECTIVE: ACL reconstruction is among the most common orthopedic procedures, yet autografts and allografts carry significant limitations including donor site morbidity and immunological risk. PET-based synthetic grafts such as LARS were introduced to address these shortcomings, but their biologically inert surface raises concerns regarding cellular adhesion and host tissue integration. A new generation of functionalized PET scaffolds has emerged over the past decade, yet no systematic review has summarized this evidence since 2011. The present review aims to evaluate the biological and mechanical effects of novel functionalized PET scaffolds for ACL reconstruction. METHODS: A systematic review was conducted in accordance with the PRISMA 2020 statement. PubMed, Embase, and Web of Science were searched up to 20 August 2026 for original studies investigating chemically or biologically modified polyethylene terephthalate grafts for Anterior cruciate ligament reconstruction and reporting in vitro outcomes, in vivo animal model data, and mechanical properties. Two independent reviewers performed screening, full-text assessment, and data extraction. RESULTS: Seventeen studies met the inclusion criteria. Functionalization strategies included physical deposition, chemical surface modification, biomimetic mineral coatings, and hybrid composite fabrication. Across all studies, functionalized PET scaffolds generally demonstrated improved performance compared with unmodified PET controls. In vitro, enhanced cellular proliferation, upregulation of osteogenic markers, and increased angiogenic activity were observed. In vivo, improved graft-bone integration was demonstrated by higher bone volume fraction, reduced tunnel area, and decreased interface width. Post-implantation ultimate load to failure and stiffness generally favored functionalized PET scaffolds, though both parameters remained inferior to the native ACL in direct comparisons. CONCLUSION: Surface biofunctionalization appears to enhance the biological and mechanical performance of PET-based scaffolds for ACL reconstruction, suggesting that surface bioengineering may play an important role in promoting biological integration and functional performance. These findings indicate that functionalized PET scaffolds could represent potential donor-independent alternatives for ACL reconstruction and may warrant further investigation as synthetic ligament substitutes. However, a persistent mechanical gap versus native ligament tissue remains. These findings should be interpreted with caution due to methodological heterogeneity, predominant use of small animal models, and inconsistent outcome reporting across the included studies. CI - Copyright (c) 2026 Bertolino, Picchi, Resta, Oliva, Fidanza and Bonanzinga. FAU - Bertolino, Luca AU - Bertolino L AD - Humanitas University, Milan, Italy. FAU - Picchi, Aurelio AU - Picchi A AD - Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy. FAU - Resta, Simonetta AU - Resta S AD - IRCCS Humanitas Research Hospital, Milan, Italy. FAU - Oliva, Paolo AU - Oliva P AD - IRCCS Humanitas Research Hospital, Milan, Italy. FAU - Fidanza, Andrea AU - Fidanza A AD - Department of Life, Health and Environmental Sciences, University of L'Aquila, L'Aquila, Italy. AD - Mini-Invasive and Computer-Assisting Orthopaedic Surgery Unit, San Salvatore Hospital, L'Aquila, Italy. FAU - Bonanzinga, Tommaso AU - Bonanzinga T AD - IRCCS Humanitas Research Hospital, Milan, Italy. AD - Department of Biomedical Sciences, Humanitas University, Milan, Italy. LA - eng PT - Journal Article PT - Systematic Review DEP - 20261014 PL - Switzerland TA - Front Bioeng Biotechnol JT - Frontiers in bioengineering and biotechnology JID - 101632513 PMC - PMC13713685 OTO - NOTNLM OT - anterior cruciate ligament reconstruction OT - cell-material interaction OT - polyethylene terephthalate OT - preclinical studies OT - scaffold OT - surface functionalization COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:12 PHST- 2026/06/22 00:00 [received] PHST- 2026/09/03 00:00 [revised] PHST- 2026/09/11 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:12 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1916072 [pii] AID - 10.3389/fbioe.2026.1916072 [doi] PST - epublish SO - Front Bioeng Biotechnol. 2026 Oct 14;14:1916072. doi: 10.3389/fbioe.2026.1916072. eCollection 2026. PMID- 42952741 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2673-6101 (Electronic) IS - 2673-6101 (Print) IS - 2673-6101 (Linking) VI - 7 DP - 2026 TI - Non-invasive prediction of olfactory recovery in severe CRSwNP: a cross-compartment translational nomogram based on secretory IL-17A. PG - 1978136 LID - 10.3389/falgy.2026.1978136 [doi] LID - 1978136 AB - BACKGROUND: Predicting olfactory recovery after surgery for severe chronic rhinosinusitis with nasal polyps (CRSwNP) remains challenging. To overcome the reliance on invasive tissue biopsies, this study aimed to identify non-invasive liquid biopsy biomarkers reflecting the deep mucosal inflammatory microenvironment, ultimately developing a point-of-care prognostic model. METHODS: A "tissue-to-secretion" dual-cohort validation strategy was employed. A Random Forest (RF) algorithm screened deep-tissue proteomes (Luminex) in a discovery cohort (N = 67). An independent RF model evaluated non-invasive nasal secretions (Olink) in a validation cohort (N = 62). Bidirectionally convergent targets were assessed for continuous dose-response relationships with absolute olfactory improvement [Delta(visual analog scale)VAS] score at 6 months. A clinical-molecular nomogram was developed and internally validated using 1,000 bootstrap resamples. RESULTS: The tissue-based RF identified interleukin (IL)-17A as a core prognostic candidate. The independent secretion-based RF model also prioritized secretory IL-17A, achieving bidirectional convergence. While canonical Type 2 cytokines (IL-5, IL-13) lost predictive significance in secretions (P > 0.05), secretory IL-17A demonstrated a robust dose-response correlation (r = 0.334, P = 0.0003) and emerged as an independent protective factor for olfactory recovery ([odds ratio] OR = 1.408, 95% [confidence interval] CI: 1.03-2.03). The joint nomogram, seamlessly integrating baseline VAS and secretory IL-17A, yielded an optimism-corrected (area under the receiver operating characteristic curve) AUC of 0.793 (95% CI: [0.6908-0.9079]) with adequate calibration and substantial net clinical benefit. CONCLUSIONS: Through cross-compartment translation, we identified secretory IL-17A as a robust, non-invasive indicator of mucosal plasticity and olfactory reversibility. The derived joint nomogram provides an actionable framework for personalized pre-operative counseling and risk stratification in severe CRSwNP. CI - (c) 2026 Zhang, Wang, Lu, Hou, Wei, Huang, Liu, Huang, Xu, Li, Wen and Zhong. FAU - Zhang, Hangtian AU - Zhang H AD - Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China. AD - Guangdong Engineering Research Center of Otorhinolaryngology Diagnosis and Treatment, Guangzhou, Guangdong, China. AD - Otorhinolaryngology Institute of Sun Yat-en University, Guangzhou, Guangdong, China. AD - Guangzhou Key Laboratory of Otorhinolaryngology, Guangzhou, Guangdong, China. FAU - Wang, Linlu AU - Wang L AD - Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China. AD - Otorhinolaryngology Institute of Sun Yat-en University, Guangzhou, Guangdong, China. AD - Guangzhou Key Laboratory of Otorhinolaryngology, Guangzhou, Guangdong, China. AD - Department of Otolaryngology-Head and Neck Surgery, The Third Affiliated Hospital of SunYat-Sen University, Guangzhou, China. FAU - Lu, Tong AU - Lu T AD - Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China. AD - Guangdong Engineering Research Center of Otorhinolaryngology Diagnosis and Treatment, Guangzhou, Guangdong, China. AD - Otorhinolaryngology Institute of Sun Yat-en University, Guangzhou, Guangdong, China. AD - Guangzhou Key Laboratory of Otorhinolaryngology, Guangzhou, Guangdong, China. AD - Department of Allergy, The First Affiliated Hospital of Sun Yat-sen University, Otorhinolaryngology Institute of Sun Yat-sen University, Guangzhou, Guangdong, China. FAU - Hou, Yilin AU - Hou Y AD - Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China. AD - Otorhinolaryngology Institute of Sun Yat-en University, Guangzhou, Guangdong, China. AD - Guangzhou Key Laboratory of Otorhinolaryngology, Guangzhou, Guangdong, China. AD - Department of Otorhinolaryngology, Peking University Shenzhen Hospital, Shenzhen Peking University-the Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong, China. FAU - Wei, Yi AU - Wei Y AD - Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China. AD - Guangdong Engineering Research Center of Otorhinolaryngology Diagnosis and Treatment, Guangzhou, Guangdong, China. AD - Otorhinolaryngology Institute of Sun Yat-en University, Guangzhou, Guangdong, China. AD - Guangzhou Key Laboratory of Otorhinolaryngology, Guangzhou, Guangdong, China. AD - Department of Allergy, The First Affiliated Hospital of Sun Yat-sen University, Otorhinolaryngology Institute of Sun Yat-sen University, Guangzhou, Guangdong, China. FAU - Huang, Wenhao AU - Huang W AD - Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China. AD - Guangdong Engineering Research Center of Otorhinolaryngology Diagnosis and Treatment, Guangzhou, Guangdong, China. AD - Otorhinolaryngology Institute of Sun Yat-en University, Guangzhou, Guangdong, China. AD - Guangzhou Key Laboratory of Otorhinolaryngology, Guangzhou, Guangdong, China. AD - Department of Allergy, The First Affiliated Hospital of Sun Yat-sen University, Otorhinolaryngology Institute of Sun Yat-sen University, Guangzhou, Guangdong, China. FAU - Liu, Shaolin AU - Liu S AD - Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China. AD - Guangdong Engineering Research Center of Otorhinolaryngology Diagnosis and Treatment, Guangzhou, Guangdong, China. AD - Otorhinolaryngology Institute of Sun Yat-en University, Guangzhou, Guangdong, China. AD - Guangzhou Key Laboratory of Otorhinolaryngology, Guangzhou, Guangdong, China. AD - Department of Allergy, The First Affiliated Hospital of Sun Yat-sen University, Otorhinolaryngology Institute of Sun Yat-sen University, Guangzhou, Guangdong, China. FAU - Huang, Yanran AU - Huang Y AD - Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China. AD - Guangdong Engineering Research Center of Otorhinolaryngology Diagnosis and Treatment, Guangzhou, Guangdong, China. AD - Otorhinolaryngology Institute of Sun Yat-en University, Guangzhou, Guangdong, China. AD - Guangzhou Key Laboratory of Otorhinolaryngology, Guangzhou, Guangdong, China. AD - Department of Allergy, The First Affiliated Hospital of Sun Yat-sen University, Otorhinolaryngology Institute of Sun Yat-sen University, Guangzhou, Guangdong, China. FAU - Xu, Zhaofeng AU - Xu Z AD - Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China. AD - Guangdong Engineering Research Center of Otorhinolaryngology Diagnosis and Treatment, Guangzhou, Guangdong, China. AD - Otorhinolaryngology Institute of Sun Yat-en University, Guangzhou, Guangdong, China. AD - Guangzhou Key Laboratory of Otorhinolaryngology, Guangzhou, Guangdong, China. AD - Department of Allergy, The First Affiliated Hospital of Sun Yat-sen University, Otorhinolaryngology Institute of Sun Yat-sen University, Guangzhou, Guangdong, China. FAU - Li, Jian AU - Li J AD - Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China. AD - Guangdong Engineering Research Center of Otorhinolaryngology Diagnosis and Treatment, Guangzhou, Guangdong, China. AD - Otorhinolaryngology Institute of Sun Yat-en University, Guangzhou, Guangdong, China. AD - Guangzhou Key Laboratory of Otorhinolaryngology, Guangzhou, Guangdong, China. AD - Department of Allergy, The First Affiliated Hospital of Sun Yat-sen University, Otorhinolaryngology Institute of Sun Yat-sen University, Guangzhou, Guangdong, China. AD - Guangxi Hospital Division of the First Affiliated Hospital, Sun Yat-sen University, Nanning, China. FAU - Wen, Weiping AU - Wen W AD - Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China. AD - Guangdong Engineering Research Center of Otorhinolaryngology Diagnosis and Treatment, Guangzhou, Guangdong, China. AD - Otorhinolaryngology Institute of Sun Yat-en University, Guangzhou, Guangdong, China. AD - Guangzhou Key Laboratory of Otorhinolaryngology, Guangzhou, Guangdong, China. AD - Department of Allergy, The First Affiliated Hospital of Sun Yat-sen University, Otorhinolaryngology Institute of Sun Yat-sen University, Guangzhou, Guangdong, China. AD - Department of Otolaryngology, The Seventh Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China. FAU - Zhong, Hua AU - Zhong H AD - Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China. AD - Guangdong Engineering Research Center of Otorhinolaryngology Diagnosis and Treatment, Guangzhou, Guangdong, China. AD - Otorhinolaryngology Institute of Sun Yat-en University, Guangzhou, Guangdong, China. AD - Guangzhou Key Laboratory of Otorhinolaryngology, Guangzhou, Guangdong, China. AD - Department of Allergy, The First Affiliated Hospital of Sun Yat-sen University, Otorhinolaryngology Institute of Sun Yat-sen University, Guangzhou, Guangdong, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Allergy JT - Frontiers in allergy JID - 9918227355906676 PMC - PMC13713674 OTO - NOTNLM OT - Type 2 inflammation OT - anosmia OT - biomarkers OT - machine learning OT - nasal mucosa COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:12 PHST- 2026/08/25 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:12 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/falgy.2026.1978136 [doi] PST - epublish SO - Front Allergy. 2026 Oct 14;7:1978136. doi: 10.3389/falgy.2026.1978136. eCollection 2026. PMID- 42952738 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-861X (Print) IS - 2296-861X (Electronic) IS - 2296-861X (Linking) VI - 13 DP - 2026 TI - Nutritional diversity and bioactive potential of thirteen wild edible insects in India: implications for sustainable food and nutrition security. PG - 1942254 LID - 10.3389/fnut.2026.1942254 [doi] LID - 1942254 AB - BACKGROUND: Edible insects are increasingly recognized as sustainable alternative food resources owing to their high nutritional value, diverse bioactive compounds, and low environmental footprint. However, comprehensive nutritional characterization of many wild edible insect species remains limited. Therefore, this study evaluated the nutritional composition, amino acid and fatty acid profiles, bioactive properties, and multivariate nutritional relationships of 13 wild-harvested edible insect species traditionally consumed in Nagaland, India. METHODS: Proximate composition, total phenolic content, antioxidant content, amino acid composition, and fatty acid profiles were determined via standard analytical methods. Spearman's correlation analysis, principal component analysis (PCA), and chord diagram visualization were employed to examine associations among nutritional variables and species-specific nutritional characteristics. RESULTS: Across all 13 species, protein content ranged from 18.90 to 63.91%, while the lipid content varied from 4.31 to 35.08%. The total phenolic content ranged from 2.80 to 13.89 mg GAE g(-1), and the antioxidant content ranged from 2.01 to 45.11 mg AAE g(-1). Eight essential and nine nonessential amino acids were detected across all species, with leucine and proline as the predominant amino acids. Fatty acid profiling revealed myristic, palmitic, stearic, palmitoleic, oleic, and linoleic acids as the major fatty acids, with monounsaturated fatty acids constituting the dominant lipid fraction (46.28-56.90 mug 100 g(-1)), followed by saturated (29.69-42.50 mug100 g(-1)) and polyunsaturated fatty acids (0.11-20.12 mug 100 g(-1)). Strong positive Spearman's correlations were observed between carbohydrate and phenolic content (rho = 0.782) and carbohydrate and antioxidant content (rho = 0.784). Phenolic and antioxidant contents were also positively correlated (rho = 0.629). Multivariate analyses, including principal component analysis and chord diagram visualization, revealed distinct species-specific nutritional profiles. Provespa barthelemyi larvae presented relatively high concentrations of essential and nonessential amino acids and oleic acid. In contrast, Oecophylla smaragdina and Omphisa fuscidentalis were more strongly associated with elevated phenolic and antioxidant contents. CONCLUSION: Our findings demonstrate significant interspecific variation in proximate composition, amino acid and fatty acid profiles, phenolic and antioxidant content, underscoring the importance of species-specific nutritional characterization when selecting edible insects for nutritional enrichment in value-added product development and their potential contribution to sustainable food systems and future food security. CI - Copyright (c) 2026 Mozhui, Kakati, Trivedi, Tanwar, Dhillon, Ao, Pankaj, Chhetri and Anal. FAU - Mozhui, Lobeno AU - Mozhui L AD - Department of Zoology, Nagaland University, Lumami, India. FAU - Kakati, L N AU - Kakati LN AD - Faculty of Science, Assam down town University, Guwahati, Assam, India. FAU - Trivedi, Neha AU - Trivedi N AD - Department of Biochemistry, College of Basic Science and Humanities, Govind Ballabh Pant University of Agriculture & Technology, Pantnagar, Uttarakhand, India. FAU - Tanwar, Aditya Kumar AU - Tanwar AK AD - Division of Entomology, IARI, New Delhi, India. FAU - Dhillon, Mukesh Kumar AU - Dhillon MK AD - Division of Entomology, IARI, New Delhi, India. FAU - Ao, Bendang AU - Ao B AD - Department of Zoology, Nagaland University, Lumami, India. FAU - Pankaj, Pranay Punj AU - Pankaj PP AD - Department of Zoology, Nagaland University, Lumami, India. FAU - Chhetri, Rita Khatri AU - Chhetri RK AD - Department of Zoology, Nagaland University, Lumami, India. FAU - Anal, Jasha Momo H AU - Anal JMH AD - Natural Products and Medicinal Chemistry Division, CSIR- Indian Institute of Integrative Medicine, Jammu, India. AD - Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Nutr JT - Frontiers in nutrition JID - 101642264 PMC - PMC13713423 OTO - NOTNLM OT - amino acids OT - edible insects OT - fatty acids OT - functional foods OT - nutritional composition OT - traditional food COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:12 PHST- 2026/07/19 00:00 [received] PHST- 2026/09/11 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:12 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fnut.2026.1942254 [doi] PST - epublish SO - Front Nutr. 2026 Oct 14;13:1942254. doi: 10.3389/fnut.2026.1942254. eCollection 2026. PMID- 42952733 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 17 DP - 2026 TI - Case Report: Zanubrutinib for refractory immune thrombotic thrombocytopenic purpura. PG - 1895825 LID - 10.3389/fimmu.2026.1895825 [doi] LID - 1895825 AB - Immune-mediated thrombotic thrombocytopenic purpura (iTTP) results from severe ADAMTS13 deficiency and may relapse despite modern therapy, with survival exceeding 95% in contemporary cohorts. We report the case of a 43-year-old patient with multiple clinical and ADAMTS13 relapses over 15 years, who developed intolerance or had incomplete responses to several immunosuppressive agents, including anti-CD20 and anti-CD38 therapies. Splenectomy was considered high risk due to significant cardiac comorbidity. Off-label zanubrutinib, a second-generation Bruton tyrosine kinase (BTK) inhibitor licensed locally for chronic lymphocytic leukemia (CLL) was initiated after the sixth relapse, which was a clinical one, simultaneously with plasma exchange (PEX) and corticosteroids. Following initiation, ADAMTS13 activity rose, platelet counts normalized, and the patient has remained in clinical remission for 20 months under zanubrutinib. This case illustrates the feasibility of BTK inhibition in selected, treatment-limited iTTP. CI - Copyright (c) 2026 De Angelis, Schnegg-Kaufmann, Moutzouri, Jalowiec, Ratknic, Kremer Hovinga and Chanias. FAU - De Angelis, Adrian Dante AU - De Angelis AD AD - Department of Hematology and Central Hematological Laboratory, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland. AD - Centre for Oncology and Hematology, Thun-Spiez-Berner Oberland, Spital STS AG, Thun, Switzerland. FAU - Schnegg-Kaufmann, Annatina AU - Schnegg-Kaufmann A AD - Department of Hematology and Central Hematological Laboratory, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland. AD - Department for BioMedical Research, University of Bern, Bern, Switzerland. FAU - Moutzouri, Elisavet AU - Moutzouri E AD - Department of Hematology and Central Hematological Laboratory, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland. AD - Department of Hematology, HFR Hopital Fribourgeois, Fribourg, Switzerland. FAU - Jalowiec, Katarzyna Aleksandra AU - Jalowiec KA AD - Department of Hematology and Central Hematological Laboratory, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland. FAU - Ratknic, Milos AU - Ratknic M AD - Department of Hematology and Central Hematological Laboratory, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland. AD - Centre of Oncology/Haematology and Transfusion Medicine, Cantonal Hospital Aarau, Aarau, Switzerland. FAU - Kremer Hovinga, Johanna A AU - Kremer Hovinga JA AD - Department of Hematology and Central Hematological Laboratory, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland. AD - Department for BioMedical Research, University of Bern, Bern, Switzerland. FAU - Chanias, Ioannis AU - Chanias I AD - Department of Hematology and Central Hematological Laboratory, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland. AD - Department of Hematology, Bone Marrow Transplantation Unit and Institute of Cell Therapy, University Hospital of Patras, Rio, Greece. LA - eng PT - Case Reports PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - EC 2.7.10.2 (Agammaglobulinaemia Tyrosine Kinase) RN - EC 3.4.24.87 (ADAMTS13 Protein) RN - EC 3.4.24.87 (ADAMTS13 protein, human) RN - 0 (Protein Kinase Inhibitors) RN - EC 2.7.10.2 (BTK protein, human) RN - 0 (Pyrazoles) RN - 0 (Pyrimidines) SB - IM MH - Humans MH - Adult MH - *Purpura, Thrombotic Thrombocytopenic/drug therapy/diagnosis MH - Agammaglobulinaemia Tyrosine Kinase/antagonists & inhibitors MH - ADAMTS13 Protein/deficiency MH - Male MH - *Protein Kinase Inhibitors/therapeutic use MH - Treatment Outcome MH - Recurrence MH - Plasma Exchange MH - *Pyrazoles/therapeutic use MH - Purpura, Thrombocytopenic, Idiopathic MH - *Pyrimidines/therapeutic use PMC - PMC13713702 OTO - NOTNLM OT - BTK inhibition OT - autoimmunity OT - chronic OT - immune TTP OT - relapse/refractory COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:11 PHST- 2026/05/30 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:11 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fimmu.2026.1895825 [doi] PST - epublish SO - Front Immunol. 2026 Oct 14;17:1895825. doi: 10.3389/fimmu.2026.1895825. eCollection 2026. PMID- 42952730 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2235-2988 (Electronic) IS - 2235-2988 (Linking) VI - 16 DP - 2026 TI - Machine learning-based risk prediction for invasive pulmonary aspergillosis in non-neutropenic intensive care unit patients, with an exploratory analysis of pathogen-related risk factors. PG - 1967577 LID - 10.3389/fcimb.2026.1967577 [doi] LID - 1967577 AB - BACKGROUND: Invasive pulmonary aspergillosis (IPA) is increasingly recognized in non-neutropenic patients, but validated bedside risk stratification tools remain scarce. We developed a machine learning model to predict IPA and tested whether corticosteroid effect depends on bacterial co-pathogen. METHODS: This multicenter retrospective cohort study enrolled 837 non-neutropenic adults hospitalized with pulmonary infections across three tertiary hospitals in China between December 2020 and December 2025. The discovery cohort was split into training and internal validation sets. Four classifiers were compared, with Random Forest selected for optimal performance. The final model was externally validated in an independent cohort. Feature importance was assessed using SHAP (SHapley Additive exPlanations) analysis. Corticosteroid-bacteria interactions were examined using subgroup analyses and propensity score matching, with the synergy index S calculated to quantify biological interaction. A simplified bedside score was derived from the top-ranking predictors. RESULTS: The Random Forest model achieved external validation AUC of 0.865. Six predictors consistently dominated across all assessments: Pseudomonas aeruginosa isolation, hypoxemia (PaO(2)/FiO(2) <200 mmHg), diabetes mellitus, organ failure (Sequential Organ Failure Assessment, SOFA >5), lymphopenia (lymphocyte count <0.8x10(9)/L), and ICU (intensive care unit) admission. Corticosteroid exposure did not rank among the top six predictors. However, its association with IPA diverged sharply by bacterial context: OR below 1 with P. aeruginosa (OR 0.52, 95% CI 0.214-1.283) but OR above 1 with other Gram-negative bacilli (OR 2.17, 95% CI 0.722-6.535); P for interaction = 0.131; a directional pattern that persisted after propensity matching. The six-variable score derived from these predictors showed AUCs of 0.869 (training), 0.774 (internal validation), and 0.719 (external validation). CONCLUSIONS: IPA risk in non-neutropenic ICU patients is shaped more by bacterial co-pathogen context than by corticosteroid exposure alone. The divergent corticosteroid effects across bacterial species challenge the conventional assumption of a uniform steroid-associated risk. The externally validated six-variable score offers a practical, software-free tool to support pathogen-informed, individualized risk stratification and clinical decision-making in the ICU. CI - Copyright (c) 2026 Bai, Feng, Liu, Wang, Wang, Liu and Zhu. FAU - Bai, Jijia AU - Bai J AD - Department of Critical Care Medicine, General Hospital of Ningxia Medical University, Yinchuan, China. FAU - Feng, Tao AU - Feng T AD - The Third People's Hospital of Ningxia, Yinchuan, Ningxia, China. FAU - Liu, Bofei AU - Liu B AD - The Fourth People's Hospital of Ningxia Hui Autonomous Region, Yinchuan, Ningxia, China. FAU - Wang, Jinyan AU - Wang J AD - The Fourth People's Hospital of Ningxia Hui Autonomous Region, Yinchuan, Ningxia, China. FAU - Wang, Zhifeng AU - Wang Z AD - The Third People's Hospital of Ningxia, Yinchuan, Ningxia, China. FAU - Liu, Furui AU - Liu F AD - The First School of Clinical Medicine, Ningxia Medical University, Yinchuan, Ningxia, China. FAU - Zhu, Jinyuan AU - Zhu J AD - Department of Critical Care Medicine, General Hospital of Ningxia Medical University, Yinchuan, China. LA - eng PT - Journal Article PT - Multicenter Study DEP - 20261014 PL - Switzerland TA - Front Cell Infect Microbiol JT - Frontiers in cellular and infection microbiology JID - 101585359 RN - 0 (Adrenal Cortex Hormones) SB - IM MH - Humans MH - *Invasive Pulmonary Aspergillosis/microbiology/diagnosis/epidemiology MH - *Intensive Care Units MH - *Machine Learning MH - Retrospective Studies MH - Risk Factors MH - Random Forest MH - Female MH - Adrenal Cortex Hormones/therapeutic use MH - Middle Aged MH - Predictive Learning Models MH - Risk Assessment/methods MH - China MH - Pseudomonas aeruginosa MH - Aged PMC - PMC13713394 OTO - NOTNLM OT - Pseudomonas aeruginosa OT - corticosteroids OT - gram-negative bacteria OT - invasive pulmonary aspergillosis OT - machine learning OT - non-neutropenic COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:11 PHST- 2026/08/14 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:11 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fcimb.2026.1967577 [doi] PST - epublish SO - Front Cell Infect Microbiol. 2026 Oct 14;16:1967577. doi: 10.3389/fcimb.2026.1967577. eCollection 2026. PMID- 42952724 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2673-4915 (Electronic) IS - 2673-4915 (Linking) VI - 7 DP - 2026 TI - Efficacy of a single-file reciprocating protocol vs. a multi-file rotary protocol on the removal of gutta-percha and ceraSeal sealer from oval canals: a volumetric and active instrumentation time analysis. PG - 1926115 LID - 10.3389/fdmed.2026.1926115 [doi] LID - 1926115 AB - OBJECTIVE: To evaluate and compare the overall efficacy and time efficiency of two complete manufacturer-recommended retreatment protocols, a single-file reciprocating protocol, Reciproc (RC), vs. a multi-file continuous rotary protocol, ProTaper Universal Retreatment (PR), in removing gutta-percha and CeraSeal sealer from oval-shaped root canals. METHODOLOGY: Thirty-six single-rooted human mandibular premolars with single oval canals were selected, instrumented, and obturated with a matching cone gutta-percha and CeraSeal sealer. Initial filling volumes were evaluated via CBCT. The samples were randomly divided into two experimental groups (n = 18) according to the file used for retreatment. The net active instrumentation time was recorded. Post-retreatment CBCT scans were performed to measure the volume and percentage of remaining filling materials. Data were statistically analyzed at a significance level of p 0.99) for detecting medium-effect differences. RESULT: The overall quality score was 2.74 +/- 0.85, with a pass rate (>/=3.5) of only 19.8%; a sensitivity analysis using alternative cutoffs (>/=3.0 and >/=4.0) yielded pass rates of 42.4% and 6.7%, respectively, confirming the robustness of the low-quality conclusion. TikTok's overall quality, content scientificity, and source credibility scores were significantly higher than MicroBlog and Today's Headlines (all P < 0.05, eta (2) = 0.11-0.16), a difference partly attributable to TikTok's higher proportion of certified physicians. One-way ANOVA revealed significant overall differences among four creator types (F = 128.6, P < 0.001, eta (2) = 0.46), with Tukey post hoc tests confirming certified physicians > rehabilitation nurses > health institutions > ordinary users. Subgroup analysis showed that sexual/reproductive rehabilitation videos had the lowest quality (2.45 +/- 0.91). A weak negative association was observed between the engagement composite and quality (r = -0.18, 95% CI: -0.27 to -0.09, P < 0.001). CONCLUSION: The overall quality of breast cancer rehabilitation popular science videos on Chinese mainstream platforms was low. Patients should prioritize content from certified physicians and health institutions that cite guidelines and present balanced information. Platform-specific regulation and greater participation by certified oncologists and rehabilitation specialists are urgently needed. LEVEL OF EVIDENCE: Level II; Cross-Sectional Study. CI - (c) 2026 Wu, Chen, Chen, Wang and Wang. FAU - Wu, Siqi AU - Wu S AD - Rehabilitation Medicine Department, The Affiliated Hospital of Southwest Medical University, Luzhou City, Sichuan Province, China. FAU - Chen, Chang AU - Chen C AD - Department of Traditional Chinese Medicine Rehabilitation, Jiangbei Campus, The First Affiliated Hospital of Army Medical University (Third Military Medical University), Chongqing, China. FAU - Chen, Ge AU - Chen G AD - Department of Orthopaedics, The Affiliated Hospital of Southwest Medical University, Luzhou City, Sichuan Province, China. FAU - Wang, Fuyou AU - Wang F AD - Department of Traditional Chinese Medicine Rehabilitation, Jiangbei Branch of The First Hospital Affiliated to Army Medical University (Third Military Medical University), Chongqing, China. FAU - Wang, Jianxiong AU - Wang J AD - Department of Rehabilitation Medicine, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, University of Electronic Science and Technology of China, Chengdu, China. AD - Rehabilitation Medicine Department, The Affiliated Hospital of Southwest Medical University, Luzhou City, Sichuan Province, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Digit Health JT - Frontiers in digital health JID - 101771889 PMC - PMC13713626 OTO - NOTNLM OT - TikTok OT - breast neoplasms OT - cancer rehabilitation OT - health literacy OT - lymphedema OT - social media COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:10 PHST- 2026/08/17 00:00 [received] PHST- 2026/09/12 00:00 [revised] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:10 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fdgth.2026.1970196 [doi] PST - epublish SO - Front Digit Health. 2026 Oct 14;8:1970196. doi: 10.3389/fdgth.2026.1970196. eCollection 2026. PMID- 42952706 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-1078 (Print) IS - 1664-1078 (Electronic) IS - 1664-1078 (Linking) VI - 17 DP - 2026 TI - Effectiveness of a brief body-oriented intervention on muscle activities when playing the violin. PG - 1944291 LID - 10.3389/fpsyg.2026.1944291 [doi] LID - 1944291 AB - BACKGROUND: Violin performance requires highly complex sensorimotor coordination, resulting in specific playing techniques and individual ergonomic strategies. Despite this adaptability, violinists frequently experience musculoskeletal problems, particularly involving muscle fatigue and overuse. A better understanding of muscle activities during violin performance and approaches to reduce muscular load remains necessary. OBJECTIVE: This study investigated the effects of a brief body-oriented intervention based on the Feldenkrais Method on muscle activity and movement patterns during violin performance. METHODS: Fourteen violinists performed a musical excerpt while upper-limb kinematics, violin position, and electromyographic (EMG) activity of selected shoulder and arm muscles were recorded. Measurements were obtained at three time points: (1) baseline, (2) following a 30-min rest period, and (3) after a 45-min Feldenkrais-based intervention. Participants also reported the presence of playing-related musculoskeletal complaints. RESULTS: The EMG activity values decreased across the three measurements in all muscle groups. However, the muscle activity of some muscle groups, including the forearm flexor muscles on both sides and the left biceps, decreased after the pause, while the muscle activity of other muscle groups, including both upper trapezius muscles and the right biceps, decreased after the intervention. The range of motion in the right arm remained unchanged across the measurements. The analysis of the violin positioning identified four distinct playing types based on the violin height (high: scroll at shoulder level; low: scroll at breast level) and angle relative to the frontal plane (approximately 20 degrees or 45 degrees ). Certain muscle activation patterns differed between these playing types. Violinists playing in a lower violin position reported shoulder and neck complaints more frequently than those performing in higher playing positions. CONCLUSION: Although the short rest period reduced activity in several muscles, the Feldenkrais-based intervention provided additional reductions in muscles that did not respond to rest alone, particularly the upper trapezius muscles, a region commonly associated with playing-related pain in violinists. The findings suggest that approaches based on body-oriented interventions are necessary to selectively reduce muscular load during violin performance. Those interventions help optimize muscle function and potentially reduce the risk of long-term musculoskeletal problems. CI - Copyright (c) 2026 Nusseck, Emslander and Spahn. FAU - Nusseck, Manfred AU - Nusseck M AD - Freiburg Institute for Musicians' Medicine, University of Music Freiburg, Medical Center and Faculty of Medicine - University of Freiburg, Freiburg Center for Music Research and Teaching, Freiburg, Germany. FAU - Emslander, Kilian AU - Emslander K AD - Freiburg Institute for Musicians' Medicine, University of Music Freiburg, Medical Center and Faculty of Medicine - University of Freiburg, Freiburg Center for Music Research and Teaching, Freiburg, Germany. FAU - Spahn, Claudia AU - Spahn C AD - Freiburg Institute for Musicians' Medicine, University of Music Freiburg, Medical Center and Faculty of Medicine - University of Freiburg, Freiburg Center for Music Research and Teaching, Freiburg, Germany. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Psychol JT - Frontiers in psychology JID - 101550902 PMC - PMC13713406 OTO - NOTNLM OT - Feldenkrais method OT - intervention OT - motion analysis OT - muscle activity OT - playing position OT - playing-related problems COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:10 PHST- 2026/07/21 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:10 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpsyg.2026.1944291 [doi] PST - epublish SO - Front Psychol. 2026 Oct 14;17:1944291. doi: 10.3389/fpsyg.2026.1944291. eCollection 2026. PMID- 42952697 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-302X (Print) IS - 1664-302X (Electronic) IS - 1664-302X (Linking) VI - 17 DP - 2026 TI - Cereal-based solid-state production and storage stability of native Trichoderma harzianum isolates for biological control applications. PG - 1991071 LID - 10.3389/fmicb.2026.1991071 [doi] LID - 1991071 AB - The effectiveness of Trichoderma-based biocontrol products depends on achieving high spore yields and maintaining viable propagules during storage. This study evaluated the effects of substrate type, isolate, and storage duration on spore production and viability of four Trichoderma harzianum isolates (BH10-2, KH5-1, MG7-2, and SH11-2) cultured on four cereal-based substrates: rice grain (RG), rice powder (RP), sorghum grain (SG), and sorghum powder (SP). The experiment was arranged in a factorial Completely Randomized Design (CRD) with three replications under laboratory conditions. Spore production was assessed 10 days after fermentation, while spore viability was determined after 30, 60, and 90 days of storage following a 7-day drying period. Colony-forming unit (CFU) data were log-transformed before analysis using two-way ANOVA for initial spore production and a linear mixed-effects model (LMM) for repeated measurements of spore viability during storage. Mean separation was performed using Tukey's HSD test at P 50 years. Sixty-two patients with ED (median age: 77 years; 30 men) received standardized instruction in PMCM. Of them, 55 were also prescribed laxatives, but 12 did not use PMCM because their symptoms improved with laxatives alone. Of the remaining 50 patients, PMCM was effective in 35 (70%), with 22 (44%) showing marked improvement. The median follow-up period was 2.0 months, and no adverse events related to PMCM were observed. CONCLUSION: PMCM is a simple and noninvasive maneuver that may represent a useful adjunctive therapy for patients with ED who are refractory to laxative therapy. CI - Copyright (c) 2026 The Japan Society of Coloproctology. FAU - Watanabe, Hideo AU - Watanabe H AD - Watanabe Hospital, Matsuyama, Ehime, Japan. FAU - Ishimaru, Kei AU - Ishimaru K AD - Department of Minimally Invasive Gastroenterology, Ehime University Graduate School of Medicine, Toon, Ehime, Japan. FAU - Ono, Yoshito AU - Ono Y AD - Watanabe Hospital, Matsuyama, Ehime, Japan. FAU - Abe, Yosuke AU - Abe Y AD - Watanabe Hospital, Matsuyama, Ehime, Japan. FAU - Takeda, Masanori AU - Takeda M AD - Watanabe Hospital, Matsuyama, Ehime, Japan. FAU - Watanabe, Mayu AU - Watanabe M AD - Watanabe Hospital, Matsuyama, Ehime, Japan. FAU - Oshikiri, Taro AU - Oshikiri T AD - Department of Gastrointestinal Surgery and Surgical Oncology, Ehime University Graduate School of Medicine, Toon, Ehime, Japan. LA - eng PT - Journal Article DEP - 20261025 PL - Japan TA - J Anus Rectum Colon JT - Journal of the anus, rectum and colon JID - 101718055 PMC - PMC13713535 OTO - NOTNLM OT - Rome IV OT - constipation OT - perianal manual compression method COIS- The authors declare that there are no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/25 CRDT- 2026/10/29 05:08 PHST- 2025/12/26 00:00 [received] PHST- 2026/06/14 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:08 [entrez] PHST- 2026/10/25 00:00 [pmc-release] AID - 10.23922/jarc.2025-128 [doi] PST - epublish SO - J Anus Rectum Colon. 2026 Oct 25;10(4):591-597. doi: 10.23922/jarc.2025-128. eCollection 2026. PMID- 42952659 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2432-3853 (Electronic) IS - 2432-3853 (Linking) VI - 10 IP - 4 DP - 2026 TI - Secondary Perianal Extramammary Paget's Disease Originating from a High-Grade Tubular Villous Adenoma Treated with Sphincter-Preserving Wide Local Excision and VY Advancement Flap: A Case Report. PG - 676-683 LID - 10.23922/jarc.2026-055 [doi] AB - Secondary extramammary Paget's disease (EMPD) of the perianal region most commonly arises from an invasive colorectal adenocarcinoma; pagetoid spread from a non-invasive precursor, such as a high-grade tubular-villous adenoma (TVA), is rare. A 70-year-old man presented with a high-grade TVA of the anal canal and concurrent perianal EMPD. Initial immunohistochemistry of the perianal lesion showed CK7(-), CK20(+), and CAM5.2(+), supporting secondary EMPD of colorectal origin. Adenoma resection achieved negative margins (R0), with no invasive malignancy on imaging or endoscopy. Given staged resection, histological continuity could not be confirmed, and the lesions were initially managed as independent entities. Wide local excision with VY flap reconstruction was subsequently performed. The excised specimen showed diffuse CK20(+) and CDX2(+) with focal CK7 positivity, suggesting a colorectal-type secondary origin. GATA3 was positive in the Paget lesion but negative in the adenoma; this isolated discordant finding is discussed in the main text. The patient has remained recurrence-free for 3 years with preserved anal function. Focal CK7 positivity should not be interpreted as evidence against a secondary origin when CK20/CDX2 are diffusely coexpressed. When the precursor is suggested to be noninvasive, sphincter-preserving wide local excision with VY flap reconstruction appears feasible as an alternative to APR. CI - Copyright (c) 2026 The Japan Society of Coloproctology. FAU - Inoue, Takahiro AU - Inoue T AD - Department of Gastrointestinal Surgery, Sapporo Kojinkai Memorial Hospital, Sapporo, Hokkaido, Japan. FAU - Uemura, Kyoichi AU - Uemura K AD - Department of Surgery, Doukikai Tomakomai Hospital, Tomakomai, Hokkaido, Japan. FAU - Mizushima, Yasuhiro AU - Mizushima Y AD - Department of Surgery, Doukikai Tomakomai Hospital, Tomakomai, Hokkaido, Japan. LA - eng PT - Case Reports PT - Journal Article DEP - 20261025 PL - Japan TA - J Anus Rectum Colon JT - Journal of the anus, rectum and colon JID - 101718055 PMC - PMC13713529 OTO - NOTNLM OT - VY advancement flap OT - extramammary Paget's disease OT - pagetoid spread OT - perianal OT - sphincter preservation OT - tubular-villous adenoma COIS- The authors declare that there are no conflicts of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/25 CRDT- 2026/10/29 05:06 PHST- 2026/04/22 00:00 [received] PHST- 2026/07/15 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:06 [entrez] PHST- 2026/10/25 00:00 [pmc-release] AID - 10.23922/jarc.2026-055 [doi] PST - epublish SO - J Anus Rectum Colon. 2026 Oct 25;10(4):676-683. doi: 10.23922/jarc.2026-055. eCollection 2026. PMID- 42952652 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1663-9812 (Print) IS - 1663-9812 (Electronic) IS - 1663-9812 (Linking) VI - 17 DP - 2026 TI - Cuban epidemic neuropathy: lessons learned from scientific and political controversies. PG - 1927625 LID - 10.3389/fphar.2026.1927625 [doi] LID - 1927625 AB - INTRODUCTION: Cuban epidemic neuropathy (CEN), which emerged in the early 1990s during Cuba's Special Period, was one of the largest neurological epidemics of the 20th century and became the subject of a scientific and political controversy. OBJECTIVE: To revisit the epidemic and examine the lessons learned from the scientific debates, institutional responses, and political context that shaped its investigation and management. DEVELOPMENT: This perspective integrates epidemiological, clinical, neuropathological, therapeutic and health evidence, to analyze competing etiological hypotheses and the influence of geopolitical and institutional factors on scientific interpretation. Accumulated evidence-including severe dietary restriction, depletion of B-group vitamins and other nutrients, increased metabolic demands, tobacco and alcohol exposure, and axonal pathology-supports a multifactorial toxic-nutritional model, over a viral agent. Nutritional depletion may have reduced mitochondrial metabolic reserve, antioxidant and detoxification capacity, and increased oxidative stress. Mitochondrial dysfunction and oxidative injury provide a convergent mechanism for selective vulnerability of the optic nerve and peripheral axons. Therapeutic implications underscore the benefit of early nutritional intervention even when the specific contributions of individual nutrients or toxins remain uncertain. The scientific controversy illustrates how geopolitical and institutional factors influenced the interpretation of science, research priorities, communication, and public health decision-making. CONCLUSION: CEN demonstrates the importance of multidisciplinary evidence, scientific independence, transparent communication, early intervention, and recognition of social determinants in neurological epidemics. These lessons remain highly relevant as Cuba today confronts renewed economic hardship and disturbing geopolitical challenges in progress. CI - Copyright (c) 2026 Gonzalez-Quevedo, Santiesteban Freixas and Figaredo Curiel. FAU - Gonzalez-Quevedo, Alina AU - Gonzalez-Quevedo A AD - Instituto de Neurologia y Neurocirugia, Havana, Cuba. FAU - Santiesteban Freixas, Rosaralis AU - Santiesteban Freixas R AD - Instituto de Neurologia y Neurocirugia, Havana, Cuba. FAU - Figaredo Curiel, Francisco H AU - Figaredo Curiel FH AD - Centro de Estudios Demograficos de la Universidad de La Habana, Havana, Cuba. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Pharmacol JT - Frontiers in pharmacology JID - 101548923 PMC - PMC13713340 OTO - NOTNLM OT - Cuban epidemic neuropathy OT - mitochondrial dysfunction OT - optic neuropathy OT - social determinants of health OT - toxic-nutritional neuropathy OT - vitamin deficiency COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:06 PHST- 2026/07/03 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:06 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1927625 [pii] AID - 10.3389/fphar.2026.1927625 [doi] PST - epublish SO - Front Pharmacol. 2026 Oct 14;17:1927625. doi: 10.3389/fphar.2026.1927625. eCollection 2026. PMID- 42952648 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 17 DP - 2026 TI - B-cell receptor repertoire remodeling in Kawasaki disease is associated with clonal expansion and distinct repertoire features in coronary artery lesions. PG - 1994376 LID - 10.3389/fimmu.2026.1994376 [doi] LID - 1994376 AB - BACKGROUND: Kawasaki disease (KD) is an acute systemic vasculitis in children, but the characteristics of the B-cell receptor (BCR) repertoire during acute disease and their association with coronary artery lesions (CALs) remain poorly understood. METHODS: High-throughput sequencing of BCR heavy-chain (BCR-H) CDR3 regions was performed in 22 children, including 9 healthy controls and 13 patients with KD, of whom 9 had no CAL and 4 had CAL. Repertoire diversity, clonal expansion, immunoglobulin isotype distribution, V-J gene usage, CDR3 characteristics, and inter-individual and cross-isotype repertoire sharing were systematically evaluated. RESULTS: KD exhibited reduced BCR repertoire diversity, with a significant decrease in IgM diversity. The repertoire showed increased contributions of high-frequency and hyperexpanded clonotypes, with the most pronounced expansion in patients with CAL. IgM displayed the strongest clonal remodeling, characterized by reduced low-frequency and increased highly abundant clonotypes. KD also showed selective alterations in IGHV usage and V-J gene pairing. CAL was associated with increased IGHA usage and changes in CDR3 amino acid composition. Substantial inter-individual CDR3 sharing was observed among KD patients, and 20 recurrent clonotypes absent from healthy controls were identified as candidate KD-associated sequences. Cross-isotype CDR3 sharing also tended to be higher in KD, particularly among patients with CAL. CONCLUSIONS: Acute KD is characterized by substantial BCR repertoire remodeling, marked by selective IgM clonal expansion, repertoire convergence, and CAL-associated IgA-biased remodeling. These findings provide new insights into humoral immune dysregulation in KD and identify candidate B-cell clonotypes for further investigation of disease pathogenesis and coronary involvement. CI - Copyright (c) 2026 Xu, Lin, Chen, Zhang, Ma, Yao and Li. FAU - Xu, Yuanyuan AU - Xu Y AD - Department of Immunology, Center of Immunomolecular Engineering, Zunyi Medical University, Zunyi, Guizhou, China. FAU - Lin, Xixiong AU - Lin X AD - Department of Immunology, Center of Immunomolecular Engineering, Zunyi Medical University, Zunyi, Guizhou, China. FAU - Chen, Yanfei AU - Chen Y AD - Department of Cardiology, the Affiliated Children's Hospital of Kunming Medical University, Kunming, Yunnan, China. FAU - Zhang, Xing AU - Zhang X AD - Department of Cardiology, the Affiliated Children's Hospital of Kunming Medical University, Kunming, Yunnan, China. FAU - Ma, Long AU - Ma L AD - Department of Immunology, Center of Immunomolecular Engineering, Zunyi Medical University, Zunyi, Guizhou, China. FAU - Yao, Xinsheng AU - Yao X AD - Department of Immunology, Center of Immunomolecular Engineering, Zunyi Medical University, Zunyi, Guizhou, China. FAU - Li, Jun AU - Li J AD - Department of Immunology, Center of Immunomolecular Engineering, Zunyi Medical University, Zunyi, Guizhou, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 0 (Receptors, Antigen, B-Cell) RN - 0 (Complementarity Determining Regions) RN - 0 (Immunoglobulin M) RN - 0 (Immunoglobulin Heavy Chains) SB - IM MH - Humans MH - *Mucocutaneous Lymph Node Syndrome/immunology/genetics MH - *Receptors, Antigen, B-Cell/genetics/immunology MH - Female MH - Male MH - Complementarity Determining Regions/genetics MH - *Coronary Artery Disease/immunology/etiology MH - *Coronary Vessels/pathology/immunology MH - Child, Preschool MH - Infant MH - *B-Lymphocytes/immunology MH - Immunoglobulin M/immunology/genetics MH - Child MH - High-Throughput Nucleotide Sequencing MH - Immunoglobulin Heavy Chains/genetics PMC - PMC13713354 OTO - NOTNLM OT - B-cell receptor repertoire OT - IgA OT - IgM OT - Kawasaki disease OT - coronary artery lesions COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:06 PHST- 2026/09/10 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:06 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fimmu.2026.1994376 [doi] PST - epublish SO - Front Immunol. 2026 Oct 14;17:1994376. doi: 10.3389/fimmu.2026.1994376. eCollection 2026. PMID- 42952645 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2673-253X (Electronic) IS - 2673-253X (Linking) VI - 8 DP - 2026 TI - GlucoSense: early diabetes risk assessment via fusion of human activity recognition and glucose prediction. PG - 1932366 LID - 10.3389/fdgth.2026.1932366 [doi] LID - 1932366 AB - Non-Insulin-Dependent Diabetes Mellitus (NIDDM) is a chronic and progressive metabolic disease characterized by chronically high blood glucose levels. It is a disease with an often asymptomatic onset, which means that a high percentage of affected individuals remain unidentified until quite far in the disease process, thereby underlining the importance of a good, reliable screening instrument for proactive detection. In this paper, GlucoSense is introduced as a single framework for early NIDDM risk stratification by combining Human Activity Recognition (HAR) with continuous blood glucose forecasting using two Graph Attention Network version 2 (GATv2)-based architectures. The first is a HAR module trained with smartphone triaxial accelerometer data that covers 23 activity classes, while the second is a glucose prediction module trained with the OhioT1DM dataset to predict glucose levels for a 30 min horizon. Unlike sequential models, GlucoSense encodes complex dependencies between movement patterns, cardiac activity, nutrition, and glycemic behaviour by modeling relationships in a graph structure. The study was longitudinal, spanning 90 days, with six participants. Three important clinical parameters, mean glucose level, time above hyperglycemic level, and glycemic coefficient of variance were calculated. The system was able to correctly identify metabolically healthy individuals and those with prediabetic patterns. The framework was cross-validated with HbA1c laboratory tests to verify the clinical consistency of the framework. Both modules were benchmarked against sequential, convolutional, attention-based, and graph-based baselines under an identical protocol with five independent runs. The glucose module lowered the root mean squared error from 31.82 mg/dL for an LSTM baseline to 25.60 mg/dL, and the activity module reached 98.50% accuracy, with every difference confirmed by paired significance testing and reported with 95% confidence intervals. Understanding glucose dynamics based on smartphone sensor data and a limited number of physiological markers, GlucoSense offers a non-invasive, scalable and cost-effective alternative to traditional clinical glucose screening. This paper demonstrates the feasibility of smartphone-based longitudinal diabetes risk assessment and motivates future large-scale population studies. CI - (c) 2026 Khan, Uddin, Shamsul Alam, Baselizadeh and Masum. FAU - Khan, Md Fokrul Islam AU - Khan MFI AD - College of Business, Westcliff University, Los Angeles, CA, United States. FAU - Uddin, Md Zia AU - Uddin MZ AD - Sustainable Communication Technologies, SINTEF Digital, Oslo, Norway. FAU - Shamsul Alam, Mohammed AU - Shamsul Alam M AD - Department of Computer Science and Engineering, International Islamic University Chittagong, Chittagong, Bangladesh. FAU - Baselizadeh, Adel AU - Baselizadeh A AD - RITMO Centre for Interdisciplinary Studies in Rhythm, Time and Motion (IFI), Department of Informatics, Faculty of Mathematics and Natural Sciences, University of Oslo, Oslo, Norway. FAU - Masum, Abdul Kadar Muhammad AU - Masum AKM AD - Department of Computer Science and Engineering, International Islamic University Chittagong, Chittagong, Bangladesh. AD - Department of Computer Science and Engineering, Southeast University, Dhaka, Bangladesh. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Digit Health JT - Frontiers in digital health JID - 101771889 PMC - PMC13713498 OTO - NOTNLM OT - NIDDM OT - continuous glucose monitoring OT - early diabetes diagnosis OT - graph attention network OT - human activity recognition OT - type 2 diabetes COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:05 PHST- 2026/07/08 00:00 [received] PHST- 2026/08/26 00:00 [revised] PHST- 2026/09/03 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:05 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fdgth.2026.1932366 [doi] PST - epublish SO - Front Digit Health. 2026 Oct 14;8:1932366. doi: 10.3389/fdgth.2026.1932366. eCollection 2026. PMID- 42952643 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2296-2565 (Electronic) IS - 2296-2565 (Linking) VI - 14 DP - 2026 TI - Current status and capabilities of cancer prevention and treatment in county-level hospitals in Fujian Province: a baseline survey under the hierarchical medical system. PG - 1955931 LID - 10.3389/fpubh.2026.1955931 [doi] LID - 1955931 AB - This paper presents an overall estimate on the current status and capabilities of cancer prevention and treatment at selected county-level hospitals in Fujian Province (southeast China) to obtain baseline data for further development, construction, and reformation of county-level cancer prevention and treatment centers under the hierarchical medical and healthcare system. A province-wide survey was conducted by Fujian Cancer Hospital from June to August 2025 using a custom-made ordinary questionnaire of 40 questions to investigate cancer-specific medical resources and the comprehensive service capabilities of 35 targeted county-level hospitals in 33 counties. Effective feedback was reported by 33 participating hospitals in 31 counties. The results are analyzed via self-programmed Python codes. The following results are obtained: (a) Twenty-five participating county-level hospitals have no independent cancer-specific wards; 29 participating county-level hospitals face a shortage of medical staff; and medical devices can meet the demand of primary diagnosis and treatment; (b) Insufficient prevention and screening is reported: 16, 9, and 12 county-level hospitals have never conducted cancer risk assessment, high-risk group management, and cancer screening, respectively; (c) Full coverage of cancer multidisciplinary teams (MDTs) has yet to be achieved, and 8 hospitals have no comprehensive treatment standardization, regulation, or implementation; (d) Bidirectional referral is still ineffective due to lack of referral regulation, and 16 participating county-level hospitals have conducted fewer than 10 cases in the past year; (e) Cancer information platforms and cancer-specific databases are still in the early stages and need substantial and sustained effort. Further development of county-level cancer prevention and treatment centers is necessary to increase medical resources for cancer prevention and treatment, enhance cancer prevention and treatment capabilities, and promote the implementation of hierarchical diagnosis and treatment in cancer-specific fields. CI - Copyright (c) 2026 Guo, Feng, Yu and Xu. FAU - Guo, Chen AU - Guo C AD - Department of Medical Administration, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, China. FAU - Feng, Qingmei AU - Feng Q AD - Department of Medical Administration, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, China. FAU - Yu, Zuotian AU - Yu Z AD - Department of Medical Administration, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, China. FAU - Xu, Haipeng AU - Xu H AD - Department of Medical Administration, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Public Health JT - Frontiers in public health JID - 101616579 SB - IM MH - Humans MH - *Neoplasms/prevention & control/therapy/diagnosis MH - China MH - *Hospitals, County/statistics & numerical data/organization & administration MH - Surveys and Questionnaires PMC - PMC13713488 OTO - NOTNLM OT - cancer prevention and treatment OT - county-level hospital OT - hierarchical medical system OT - medical resources OT - survey COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:04 PHST- 2026/08/02 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:04 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpubh.2026.1955931 [doi] PST - epublish SO - Front Public Health. 2026 Oct 14;14:1955931. doi: 10.3389/fpubh.2026.1955931. eCollection 2026. PMID- 42952639 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2673-4915 (Electronic) IS - 2673-4915 (Linking) VI - 7 DP - 2026 TI - Sensory-based non-pharmacological interventions for reducing dental anxiety and improving behavioural cooperation in paediatric patients: a systematic review with GRADE assessment. PG - 1895336 LID - 10.3389/fdmed.2026.1895336 [doi] LID - 1895336 AB - BACKGROUND AND OBJECTIVES: Dental anxiety affects an estimated 9%-20% of children and disproportionately impacts those with neurodevelopmental conditions. Sensory-based non-pharmacological interventions, which intentionally modulate auditory, visual, tactile, olfactory, or proprioceptive input, have emerged as promising approaches for anxiety and behaviour management in paediatric dentistry, but no published review has applied formal GRADE certainty assessment across multiple sensory-based modalities. This review evaluated the effectiveness of sensory-based interventions for dental anxiety and behavioural cooperation in paediatric patients (/=153 and >/=433 copies/mL, respectively). CMV testing was weekly until day +100 post-HSCT; EBV testing followed clinical indication at the treating physician's discretion. IgG serostatus is not routinely tested at KHCC for post-transplant monitoring. Acknowledging some missing values, we evaluated the incidence, risk factors, and survival impact of CMV/EBV co-infection using chi-square tests, Kaplan-Meier analysis, and multivariable logistic and Cox proportional-hazards regression. RESULTS: Among 941 recipients (56.5% adult, 43.5% pediatric), adults received 89.1% of CMV tests (838/941) and 76.6% of CMV infections (105/137), whereas pediatric patients accounted for 59.8% of EBV infections (76/127); EBV testing covered 65.5% (616/941). Co-infection incidence was 7.6% (70/916). Co-infection was significantly associated with allogeneic transplant (p=0.004), haploidentical donor (p=0.010), reduced-intensity conditioning (p=0.017), and acute graft-versus-host disease (aGVHD) (p=0.028), but not with malignancy status (54/70 [77.1%] vs. 577/760 [75.9%] malignant; p=0.819); haploidentical (vs. related) donor type remained independent on multivariable analysis (adjusted OR 2.01, 95% CI 1.18-3.41, p=0.0098). CMV monoinfection was associated with adult age and haploidentical transplantation; EBV monoinfection with pediatric age, non-malignant disease, and aGVHD. Pediatric 3-year overall survival (OS) was superior (78.4% vs. 64.6%; median follow-up 41.8 months). The difference in 3-year OS between co-infected (67.3%) and non-co-infected (70.9%) patients did not reach statistical significance (Kaplan-Meier log-rank p = 0.4046; crude mortality by Pearson chi-square, p = 0.286); chronic GVHD was the only independent predictor of OS (adjusted HR 0.016, 95% CI 0.002-0.150, p=0.0003). CONCLUSION: CMV and EBV post-HCT exhibit distinct, age-associated risk profiles, and co-infection defines a high-risk phenotype linked to intensive immunosuppression and aGVHD. Three-year OS did not differ significantly between groups; given the observational design, selection bias from non-uniform EBV testing, and possible type II error, this should not be taken as evidence that pre-emptive strategies eliminate the survival impact of co-infection. Findings support age- and risk-adapted viral monitoring. CI - Copyright (c) 2026 Alsmadi, Hashem, Halahleh, Jarrah, Talab, Sultan and Al-Hussaini. FAU - Alsmadi, Osama AU - Alsmadi O AD - Department of Cell Therapy and Applied Genomics, King Hussein Cancer Center, Amman, Jordan. AD - Faculty of Allied Medical Sciences, Applied Science University, Amman, Jordan. FAU - Hashem, Hasan AU - Hashem H AD - Department of Internal Medicine, King Hussein Cancer Center, Amman, Jordan. FAU - Halahleh, Khalid AU - Halahleh K AD - Department of Internal Medicine, King Hussein Cancer Center, Amman, Jordan. FAU - Jarrah, Jude AU - Jarrah J AD - Department of Internal Medicine, King Hussein Cancer Center, Amman, Jordan. FAU - Talab, Yazan AU - Talab Y AD - Department of Cell Therapy and Applied Genomics, King Hussein Cancer Center, Amman, Jordan. FAU - Sultan, Iyad AU - Sultan I AD - Artificial Intelligence and Data Innovation Office, King Hussein Cancer Center, Amman, Jordan. FAU - Al-Hussaini, Maysa AU - Al-Hussaini M AD - Department of Cell Therapy and Applied Genomics, King Hussein Cancer Center, Amman, Jordan. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Cell Infect Microbiol JT - Frontiers in cellular and infection microbiology JID - 101585359 SB - IM MH - Humans MH - *Hematopoietic Stem Cell Transplantation/adverse effects MH - *Epstein-Barr Virus Infections/epidemiology MH - *Cytomegalovirus Infections/epidemiology MH - Female MH - Retrospective Studies MH - Adult MH - *Coinfection/epidemiology/virology MH - Male MH - Child MH - Adolescent MH - Child, Preschool MH - Middle Aged MH - Herpesvirus 4, Human MH - Risk Factors MH - Cytomegalovirus MH - Incidence MH - Young Adult MH - Infant MH - Graft vs Host Disease MH - Aged MH - *Transplant Recipients PMC - PMC13713334 OTO - NOTNLM OT - EBV_CMV OT - GvHD (graft-versus-host disease) OT - adult OT - co-infection OT - pediatrics OT - stem cell transplant (SCT) COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:03 PHST- 2026/01/22 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:03 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fcimb.2026.1793798 [doi] PST - epublish SO - Front Cell Infect Microbiol. 2026 Oct 14;16:1793798. doi: 10.3389/fcimb.2026.1793798. eCollection 2026. PMID- 42952604 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 17 DP - 2026 TI - Admission immune-inflammatory biomarker profiles for trauma severity stratification: an externally validated machine learning study. PG - 1950721 LID - 10.3389/fimmu.2026.1950721 [doi] LID - 1950721 AB - BACKGROUND: Traumatic injury induces rapid systemic immune-inflammatory alterations, but whether routinely measurable admission biomarkers can provide an early biological estimate of anatomical injury burden remains uncertain. This study aimed to characterize trauma-associated immune-inflammatory profiles across trauma severity levels defined by injury severity score (ISS) and evaluate their combined value for early severity stratification. METHODS: This two-center retrospective study included healthy participants as a physiological reference group, non-trauma acute-care patients as a clinically relevant comparator, and trauma patients classified as mild (ISS <16), moderate (ISS 16-25), or severe (ISS >25). Nineteen admission laboratory biomarkers and demographic variables were evaluated in a development cohort of 775 participants and an independent external validation cohort of 349 participants. Multinomial least absolute shrinkage and selection operator regression was used to identify severity-associated biomarkers. Ten classifiers were compared on the same predefined trauma-only development split, and the selected model was evaluated in an independent external trauma cohort. RESULTS: A 10-feature panel was retained following feature selection and collectively defined an immune-inflammatory profile associated with increasing trauma severity. Increasing injury burden was associated with heterogeneous and partly nonlinear inflammatory and hematologic alterations, including changes in neutrophil-related indices, lymphocyte-related measures, and albumin. The multinomial logistic regression model achieved the best overall performance, with an accuracy of 0.941 and 0.973 in the internal holdout and external validation cohorts, respectively, with an external macro-AUC of 0.993. Model interpretation consistently identified neutrophil count and RDW-CV as major contributors to severity stratification. CONCLUSION: Admission-stage blood biomarkers capture reproducible immune-inflammatory alterations associated with increasing anatomical injury severity. Their combined analysis may provide a complementary approach for early biological assessment of injury burden and supports further investigation of clinically accessible biomarkers of trauma-associated immune-inflammatory dysregulation. CI - Copyright (c) 2026 Dai, Zhang, Wu, Xu, Chen, Zhou, Xiao, Wu, Xiang, He, Bao and Huang. FAU - Dai, Xianling AU - Dai X AD - Department of Clinical Laboratory Medicine, First Affiliated Hospital, Army Medical University, Chongqing, China. FAU - Zhang, Ligai AU - Zhang L AD - Department of Clinical Laboratory Medicine, First Affiliated Hospital, Army Medical University, Chongqing, China. FAU - Wu, Zhiyu AU - Wu Z AD - Department of Clinical Laboratory Medicine, Chongqing Banan Traditional Chinese Medicine Hospital, Chongqing, China. FAU - Xu, Hanqing AU - Xu H AD - Department of Clinical Laboratory Medicine, First Affiliated Hospital, Army Medical University, Chongqing, China. FAU - Chen, Bin AU - Chen B AD - Department of Clinical Laboratory Medicine, First Affiliated Hospital, Army Medical University, Chongqing, China. FAU - Zhou, Yang AU - Zhou Y AD - Department of Clinical Laboratory Medicine, First Affiliated Hospital, Army Medical University, Chongqing, China. FAU - Xiao, Ye AU - Xiao Y AD - Health Management Center, First Affiliated Hospital, Army Medical University, Chongqing, China. FAU - Wu, Yu AU - Wu Y AD - Department of Clinical Laboratory Medicine, First Affiliated Hospital, Army Medical University, Chongqing, China. FAU - Xiang, Shiqing AU - Xiang S AD - Department of Clinical Laboratory Medicine, First Affiliated Hospital, Army Medical University, Chongqing, China. FAU - He, Jian AU - He J AD - Department of Respiratory and Critical Care Medicine, First Affiliated Hospital, Army Medical University, Chongqing, China. FAU - Bao, Jing AU - Bao J AD - Department of Clinical Laboratory Medicine, First Affiliated Hospital, Army Medical University, Chongqing, China. FAU - Huang, Yuting AU - Huang Y AD - Department of Clinical Laboratory Medicine, First Affiliated Hospital, Army Medical University, Chongqing, China. LA - eng PT - Journal Article PT - Multicenter Study DEP - 20261014 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 0 (Biomarkers) SB - IM MH - Humans MH - *Biomarkers/blood MH - *Machine Learning MH - Female MH - *Wounds and Injuries/immunology/diagnosis/blood MH - Retrospective Studies MH - Male MH - Injury Severity Score MH - Adult MH - Middle Aged MH - *Inflammation/immunology/blood MH - Trauma Severity Indices PMC - PMC13713292 OTO - NOTNLM OT - external validation OT - immune-inflammatory biomarkers OT - injury severity OT - machine learning OT - trauma COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 15:20 MHDA- 2026/10/29 15:21 PMCR- 2026/10/14 CRDT- 2026/10/29 05:02 PHST- 2026/07/28 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/29 15:21 [medline] PHST- 2026/10/29 15:20 [pubmed] PHST- 2026/10/29 05:02 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fimmu.2026.1950721 [doi] PST - epublish SO - Front Immunol. 2026 Oct 14;17:1950721. doi: 10.3389/fimmu.2026.1950721. eCollection 2026. PMID- 42952596 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2674-0109 (Electronic) IS - 2674-0109 (Linking) VI - 6 DP - 2026 TI - Acute cardiovascular responses to slow and deep breathing in normotensive non-pregnant and pregnant women. PG - 1913383 LID - 10.3389/fnetp.2026.1913383 [doi] LID - 1913383 AB - OBJECTIVE: To compare the acute cardiovascular responses of non-pregnant and pregnant women to slow and deep breathing (SDB) at a range of breathing frequencies. Participants: Forty-one women (23 non-pregnant and 18 pregnant). METHODS: Participants completed five different breathing conditions in a randomised order (5-min duration); spontaneous breathing (Sf (r) ), dynamic SDB breathing frequency (Df (r) ), and three fixed breathing frequencies of 4 breaths(.) min(-1) (4Ff (r) ), 6 breaths. min(-1) (6Ff (r) ), and 8 breaths. min(-1) (8Ff (r) ). Main Outcome Measures: Non-invasive beat-to-beat arterial blood pressure, heart rate and respired airflow were monitored continuously. RESULTS: The maximum amplitude of respiratory sinus arrhythmia (RSA) during SDB was 2.1 times higher than during spontaneous breathing (Sf (r) ) for both groups, maximising at 6 breaths(.) min(-1) (0.25s +/- 0.1 non-pregnant, 0.15s +/- 0.1 pregnant). Although absolute RSA was significantly lower in pregnant women during spontaneous breathing (Sf (r) ; 0.07s vs. 0.12s non-pregnant), SDB increased RSA to the same level as non-pregnant women's spontaneous breathing levels. In both groups, the amplitude of blood pressure oscillations (both systolic and diastolic blood pressure) increased as breathing frequency declined, reaching a peak at 4 breaths(.) min(-1) (4Ff (r) ), which was up to 4.5 times higher (14.3%, 10 mmHg) than during spontaneous breathing (Sf (r) ). CONCLUSION: Cardiovascular responses maximised at /= 30) and the highest number of allele calls. IonTorrent data had quality issues that impacted allele calls and clustering of epidemiologically related isolates. Therefore, sequencing using the Illumina platform will be supported moving forward as part of our national workflow. wgMLST and hqSNPs produced comparable results using Illumina data, and related strains could be distinguished from unrelated strains that were genetically similar and harbored the same toxin subtype. Therefore, a central, wgMLST database will allow U.S. public health laboratories to analyze their own sequencing data and submit data to a national database in PulseNet, the U.S. National molecular surveillance system for foodborne, waterborne, and One Health related outbreaks or events. CI - Copyright (c) 2026 Gladney, Halpin, Carleton, Pouseele and Luquez. FAU - Gladney, Lori M AU - Gladney LM AD - Enteric Diseases Laboratory Branch, Division of Foodborne, Waterborne and Environmental Diseases, National Center for Emerging Zoonotic and Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, United States. FAU - Halpin, Jessica L AU - Halpin JL AD - Enteric Diseases Laboratory Branch, Division of Foodborne, Waterborne and Environmental Diseases, National Center for Emerging Zoonotic and Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, United States. FAU - Carleton, Heather A AU - Carleton HA AD - Enteric Diseases Laboratory Branch, Division of Foodborne, Waterborne and Environmental Diseases, National Center for Emerging Zoonotic and Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, United States. FAU - Pouseele, Hannes AU - Pouseele H AD - Biomerieux Benelux, Schaerbeek, Belgium. FAU - Luquez, Carolina AU - Luquez C AD - Enteric Diseases Laboratory Branch, Division of Foodborne, Waterborne and Environmental Diseases, National Center for Emerging Zoonotic and Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, United States. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Microbiol JT - Frontiers in microbiology JID - 101548977 PMC - PMC13713324 OTO - NOTNLM OT - Clostridium botulinum OT - botulism OT - foodborne investigation OT - high-quality SNP typing OT - wgMLST COIS- The author(s) declare that the research was conducted in collaboration with BioMerieux, the company that owns BioNumerics, the proprietary software platform used to construct and distribute the wgMLST schema. HP was employed by BioMerieux Benelux. The remaining author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/14 CRDT- 2026/10/29 05:00 PHST- 2026/06/26 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 05:00 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fmicb.2026.1920805 [doi] PST - epublish SO - Front Microbiol. 2026 Oct 14;17:1920805. doi: 10.3389/fmicb.2026.1920805. eCollection 2026. PMID- 42952565 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2673-4842 (Electronic) IS - 2673-4842 (Linking) VI - 7 DP - 2026 TI - Maxillary foreign body management in odontogenic sinusitis: an algorithm based on a single centre experience. PG - 1945885 LID - 10.3389/froh.2026.1945885 [doi] LID - 1945885 AB - PURPOSE: This study aimed to evaluate the management of patients with odontogenic maxillary sinusitis associated with foreign bodies. METHODS: We retrospectively reviewed 165 patients treated at our institution; 48 met the inclusion criteria and were analyzed. All presented odontogenic sinusitis with a maxillary sinus foreign body and underwent surgery according to a multidisciplinary treatment algorithm based on clinical and radiological findings. RESULTS: Thirty-two patients were treated with a simultaneous combined oral and nasal approach, 12 with an oral approach alone, and 4 with a nasal approach alone. Complete removal of the foreign body and complete symptom resolution were achieved in all cases. CONCLUSION: In this retrospective series, surgical treatment was successful in all 48 patients. Our results support that a multidisciplinary approach was the most frequently utilized strategy in our cohort and proved highly effective in appropriately selected patients for the management of odontogenic sinusitis associated with maxillary foreign bodies, allowing surgeons to tailor the surgical approach to the specific anatomical and clinical presentation. CI - (c) 2026 Sabatino, Lopez, Valentini, Mattaroccia, Iafrati, Di Giovanni, Moffa and Casale. FAU - Sabatino, Lorenzo AU - Sabatino L AD - Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy. FAU - Lopez, Michele Antonio AU - Lopez MA AD - Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy. AD - Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy. FAU - Valentini, Matteo AU - Valentini M AD - Universita Campus Bio-Medico di Roma, Rome, Italy. FAU - Mattaroccia, Marco AU - Mattaroccia M AD - Universita Campus Bio-Medico di Roma, Rome, Italy. FAU - Iafrati, Francesco AU - Iafrati F AD - Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy. FAU - Di Giovanni, Simone AU - Di Giovanni S AD - Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy. FAU - Moffa, Antonio AU - Moffa A AD - Universita Campus Bio-Medico di Roma, Rome, Italy. FAU - Casale, Manuele AU - Casale M AD - Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy. AD - Universita Campus Bio-Medico di Roma, Rome, Italy. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Oral Health JT - Frontiers in oral health JID - 9918227262706676 PMC - PMC13713381 OTO - NOTNLM OT - endoscopic middle meatus antrostomy OT - maxillary sinus foreign body OT - modified mini Caldwell-Luc OT - multidisciplinary surgery OT - odontogenic sinusitis OT - treatment algorithm COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/14 CRDT- 2026/10/29 05:00 PHST- 2026/07/22 00:00 [received] PHST- 2026/09/13 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 05:00 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/froh.2026.1945885 [doi] PST - epublish SO - Front Oral Health. 2026 Oct 14;7:1945885. doi: 10.3389/froh.2026.1945885. eCollection 2026. PMID- 42952563 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-1078 (Print) IS - 1664-1078 (Electronic) IS - 1664-1078 (Linking) VI - 17 DP - 2026 TI - Cognitive transparency and trust calibration in explainable AI systems: a psychological model of human-AI decision reliance. PG - 1924913 LID - 10.3389/fpsyg.2026.1924913 [doi] LID - 1924913 AB - INTRODUCTION: Explainable artificial intelligence (XAI) is widely expected to improve transparency and support appropriate human reliance on AI-assisted decision-making. However, the psychological processes through which explainability relates to reliance remain insufficiently understood, and explainability, transparency, and trust have largely been examined separately. OBJECTIVES: This study develops and tests a psychological model of cognitive transparency in which AI explainability, algorithmic transparency, and perceived AI accuracy are associated with human reliance on AI through cognitive understanding, cognitive trust, and trust calibration, with AI literacy as a boundary condition. METHODS: A cross-sectional online survey was completed by 342 AI users in South Korea in May and June 2026. Each participant evaluated one self-selected AI system that they had personally used; all constructs were measured by self-report. The data were analyzed using partial least squares structural equation modeling (PLS-SEM) in SmartPLS 4. RESULTS: AI explainability (beta = 0.349) and algorithmic transparency (beta = 0.297) were positively associated with cognitive understanding. Cognitive understanding (beta = 0.388) and, more strongly, perceived AI accuracy (beta = 0.492) were associated with cognitive trust. Cognitive trust was associated with trust calibration (beta = 0.631), which was associated with human reliance on AI (beta = 0.600). All hypothesized indirect effects were significant, and AI literacy strengthened the association between AI explainability and cognitive understanding (beta = 0.192). The model explained 36%-54% of the variance in the endogenous constructs and showed medium predictive power. CONCLUSION: The findings are consistent with a sequence in which explainable AI relates to reliance through users' understanding and trust judgments. Because all constructs were self-reported and measured at a single point in time, the results describe associations among users' perceptions rather than causal effects, independently verified calibration, or objectively appropriate reliance. The findings suggest that AI developers in domains such as healthcare, education, and finance, where calibrated reliance is critical, should pair user-facing explanations with information about system accuracy and with AI literacy support. CI - Copyright (c) 2026 Rehman. FAU - Rehman, Usman AU - Rehman U AD - Department of Global Venture Technology Management, Hoseo University, Seoul, Republic of Korea. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Psychol JT - Frontiers in psychology JID - 101550902 PMC - PMC13713401 OTO - NOTNLM OT - AI literacy OT - AI trust OT - algorithmic decision-making OT - cognitive transparency OT - decision reliance OT - explainable artificial intelligence (XAI) OT - human-AI interaction OT - trust calibration COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/14 CRDT- 2026/10/29 05:00 PHST- 2026/07/01 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 05:00 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpsyg.2026.1924913 [doi] PST - epublish SO - Front Psychol. 2026 Oct 14;17:1924913. doi: 10.3389/fpsyg.2026.1924913. eCollection 2026. PMID- 42952560 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2234-943X (Print) IS - 2234-943X (Electronic) IS - 2234-943X (Linking) VI - 16 DP - 2026 TI - Predicting recurrence risk after neoadjuvant therapy in breast cancer: a single-center retrospective cohort study integrating residual cancer burden and immune microenvironment features. PG - 1925326 LID - 10.3389/fonc.2026.1925326 [doi] LID - 1925326 AB - BACKGROUND: Residual cancer burden (RCB) after neoadjuvant therapy (NAT) provides prognostic information, but its combination with immune microenvironment features for recurrence prediction remains underexplored. METHODS: This single-center retrospective cohort study included 420 consecutive patients with breast cancer who underwent neoadjuvant therapy followed by definitive surgery between January 2015 and June 2024. Residual cancer burden (RCB) and post-neoadjuvant immune features, including tumor-infiltrating lymphocytes (TILs), CD8, FOXP3, and the proportion of CD163-positive among CD68-positive macrophages, were assessed. Patients were stratified by calendar era and randomly partitioned within strata into a training cohort (n=294) and an internal hold-out validation cohort (n=126). A LASSO-penalized Cox workflow was used for predictor selection, followed by unpenalized Cox refitting. Model performance was evaluated using C-statistics, time-specific Brier scores, calibration, bootstrap internal validation, and 5-year time-dependent decision curve analysis. RESULTS: During a median follow-up of 61 months, 126 first recurrence events were observed (88 training and 38 validation). The final combined model retained RCB category, triple-negative breast cancer (TNBC), CD8 status, and CD163-positive macrophage proportion. The combined model achieved C-statistics of 0.79 (95% CI 0.75-0.83) in the training cohort and 0.75 (95% CI 0.69-0.81) in the internal validation cohort, with corresponding 5-year Brier scores of 0.142 and 0.158. Bootstrap internal validation yielded an optimism-corrected C-statistic of 0.772 and calibration slope of 0.92. The combined model provided the greatest net benefit among the evaluated models over 5-year threshold probabilities of approximately 15%-45%. CONCLUSIONS: Integration of RCB with TNBC status and post-neoadjuvant CD8 and CD163-positive macrophage features may provide incremental prognostic information for recurrence after neoadjuvant therapy. The model showed acceptable internal discrimination and calibration, but external validation is required before clinical implementation or treatment-directed use. CI - Copyright (c) 2026 Zhao, Deng, Bai, Duan and Yi. FAU - Zhao, Zhihua AU - Zhao Z AD - Physical Examination Center, Wang Jiang Hospital, Sichuan University, Chengdu, Sichuan, China. FAU - Deng, Rui AU - Deng R AD - Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan, China. FAU - Bai, Liangliang AU - Bai L AD - Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan, China. FAU - Duan, Xiaogang AU - Duan X AD - Pharmacy Department, Chengdu Eighth People's Hospital, Chengdu, Sichuan, China. FAU - Yi, Jingyuan AU - Yi J AD - General Inpatient Department, West China Health Care Hospital, Sichuan University, Chengdu, Sichuan, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Oncol JT - Frontiers in oncology JID - 101568867 PMC - PMC13713326 OTO - NOTNLM OT - breast cancer OT - immune microenvironment OT - neoadjuvant therapy OT - residual cancer burden OT - tumor-infiltrating lymphocytes COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/14 CRDT- 2026/10/29 05:00 PHST- 2026/07/01 00:00 [received] PHST- 2026/09/08 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 05:00 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fonc.2026.1925326 [doi] PST - epublish SO - Front Oncol. 2026 Oct 14;16:1925326. doi: 10.3389/fonc.2026.1925326. eCollection 2026. PMID- 42952555 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2297-055X (Print) IS - 2297-055X (Electronic) IS - 2297-055X (Linking) VI - 13 DP - 2026 TI - Developing and evaluating machine learning models in the prediction of recurrent sudden cardiac arrest. PG - 1883279 LID - 10.3389/fcvm.2026.1883279 [doi] LID - 1883279 AB - BACKGROUND: Sudden cardiac arrest (SCA) is a major killer of adults in the United States and has a significant recurrence rate. Predicting the risk of recurrent SCA has implications to patients' counseling and management. OBJECTIVE: The goal of this study is to develop and evaluate machine learning (ML) models to predict recurrent SCA in this high-risk population. METHODS: Patients (n = 5,286) whose index arrest was coded as a primary diagnosis were selected and followed to the endpoint of second SCA. The cohort was divided into training (n = 3,382, 3.8% event rate), internal validation (n = 846, 3.8% event rate), and holdout test (1,058, 3.8% event rate) sets. External validation was performed using a cohort of 1,776 patients (2.3% event rate). Multiple ML discriminators were tested using model area under the receiver operating characteristic curve (AUROC), sensitivity, specificity, positive (PPV) and negative (NPV) predictive values, and F1 scores. The model with highest AUROC was evaluated on a holdout test set and was externally validated. RESULTS: The gradient boosting (GB) model outperformed other models with AUROC of 0.93 in the internal validation set. It also achieved high discrimination in the holdout (AUROC=0.92), and external validation (AUROC = 0.90, sensitivity = 0.92, specificity = 0.83, F1 score = 0.19) sets. Shapley additive explanations (SHAP) demonstrated that a diagnostic code of shockable rhythm (ventricular tachycardia), evidence of structural heart disease, conduction abnormalities, and initial blood work results at the time of the index arrest were main contributors to model prediction. Low (NPV >/= 99%), intermediate, and high (PPV>/=25%) risk groups defined from the internal validation set were applied to the holdout and external validation sets with strong prediction of time to recurrent SCA (p = 0.003). CONCLUSION: Our ML prediction model demonstrates very good and stable discrimination in the prediction of recurrent SCA based on explainable clinical factors. External validation results confirm the generalizability of our findings. This model could support risk stratification and counseling for survivors of SCA. CI - (c) 2026 Gohel, Daidone, Gokhale, Riek, Thoma, Mulukutla, Moon, Patel, Jain, Akcakaya, Al-Zaiti and Saba. FAU - Gohel, Paulomi AU - Gohel P AD - Department of Medicine, Division of Cardiology and the Heart and Vascular Institute at the University of Pittsburgh Medical Center, Pittsburgh, PA, United States. FAU - Daidone, Michael AU - Daidone M AD - Department of Medicine, Division of Cardiology and the Heart and Vascular Institute at the University of Pittsburgh Medical Center, Pittsburgh, PA, United States. FAU - Gokhale, Tanmay A AU - Gokhale TA AD - Department of Medicine, Division of Cardiology and the Heart and Vascular Institute at the University of Pittsburgh Medical Center, Pittsburgh, PA, United States. FAU - Riek, Nathan AU - Riek N AD - School of Engineering at the University of Pittsburgh, Pittsburgh, PA, United States. FAU - Thoma, Floyd AU - Thoma F AD - Department of Medicine, Division of Cardiology and the Heart and Vascular Institute at the University of Pittsburgh Medical Center, Pittsburgh, PA, United States. FAU - Mulukutla, Suresh AU - Mulukutla S AD - Department of Medicine, Division of Cardiology and the Heart and Vascular Institute at the University of Pittsburgh Medical Center, Pittsburgh, PA, United States. FAU - Moon, Seung J AU - Moon SJ AD - Department of Medicine, Division of Cardiology and the Heart and Vascular Institute at the University of Pittsburgh Medical Center, Pittsburgh, PA, United States. FAU - Patel, Chinmay AU - Patel C AD - Department of Medicine, Division of Cardiology and the Heart and Vascular Institute at the University of Pittsburgh Medical Center, Pittsburgh, PA, United States. FAU - Jain, Sandeep K AU - Jain SK AD - Department of Medicine, Division of Cardiology and the Heart and Vascular Institute at the University of Pittsburgh Medical Center, Pittsburgh, PA, United States. FAU - Akcakaya, Murat AU - Akcakaya M AD - School of Engineering at the University of Pittsburgh, Pittsburgh, PA, United States. FAU - Al-Zaiti, Salah AU - Al-Zaiti S AD - School of Nursing, University of Rochester, Rochester, NY, United States. FAU - Saba, Samir AU - Saba S AD - Department of Medicine, Division of Cardiology and the Heart and Vascular Institute at the University of Pittsburgh Medical Center, Pittsburgh, PA, United States. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Cardiovasc Med JT - Frontiers in cardiovascular medicine JID - 101653388 PMC - PMC13713392 OTO - NOTNLM OT - machine learning OT - prediction models OT - recurrence OT - sudden cardiac arrest OT - ventricular arrhythmias COIS- SS reports research support and consultation work from Abbott, Boston Scientific and Medtronic; SJ reports research support from Boston Scientific; CP reports research support from Boston Scientific. The remaining author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/14 CRDT- 2026/10/29 04:59 PHST- 2026/05/16 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:59 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fcvm.2026.1883279 [doi] PST - epublish SO - Front Cardiovasc Med. 2026 Oct 14;13:1883279. doi: 10.3389/fcvm.2026.1883279. eCollection 2026. PMID- 42952550 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2234-943X (Print) IS - 2234-943X (Electronic) IS - 2234-943X (Linking) VI - 16 DP - 2026 TI - Barriers to survivorship care for adolescent and young adult cancer survivors: perspectives of survivors and cancer specialists. PG - 1977660 LID - 10.3389/fonc.2026.1977660 [doi] LID - 1977660 AB - BACKGROUND: Adolescent and young adult (AYA) cancer survivors have complex long-term survivorship needs beyond cancer recurrence surveillance. Although survivor concerns and healthcare provider perspectives have been reported separately, few studies have evaluated both within the same healthcare setting. We conducted complementary surveys of AYA cancer survivors and physicians to characterize survivorship concerns, barriers to long-term follow-up (LTFU), and telemedicine preferences. METHODS: This single-center, cross-sectional study included questionnaire surveys of 98 AYA cancer survivors receiving outpatient follow-up after cancer treatment and 45 physicians involved in AYA survivorship care at the National Cancer Center Hospital, Japan. Survivors were surveyed regarding LTFU, survivorship concerns, and telemedicine preferences, and physicians regarding LTFU practices, referral barriers, and AYA-specific challenges. Associations between survivor characteristics and telemedicine preference were explored using univariable analyses. RESULTS: Among survivors, 84 of 98 (85.7%) considered LTFU necessary, and 51 (52.0%) reported difficulty attending follow-up because of school or work commitments. Of 91 survivors evaluable for telemedicine preference, 69 (75.8%) were likely or somewhat likely to use at least one telemedicine service. Among all 98 survivors, frequently selected telemedicine topics included disease information (45.9%), daily activities (31.6%), anxiety and worry (30.6% each), financial issues (29.6%), and work- or school-related concerns (27.6%). No demographic, clinical, treatment-related or healthcare utilization factors were significantly associated with telemedicine preference. Among physicians, 13 of 44 (29.5%) reported an established departmental LTFU policy. Follow-up at the treating cancer center was supported by 76.2% for survivors at high risk of recurrence but by only 21.4% for all AYA survivors. Major barriers to coordinated care included difficulty identifying hospitals providing cancer-specific LTFU (73.8%) or multidisciplinary care (71.4%). Physicians identified reproductive medicine and life events as major AYA-specific challenges and mental health, endocrine disorders, and sexual and reproductive health as areas requiring additional support. CONCLUSIONS: AYA cancer survivors reported substantial survivorship concerns, whereas physicians identified barriers to coordinated multidisciplinary care. Telemedicine was of interest to many survivors and warrants prospective evaluation as a potential component of multidisciplinary survivorship care. CI - Copyright (c) 2026 Kojima, Ando, Nogami, Iwata, Suzuki and Satomi. FAU - Kojima, Yuki AU - Kojima Y AD - Department of Medical Oncology, National Cancer Center Hospital, Tokyo, Japan. FAU - Ando, Yayoi AU - Ando Y AD - Clinical Research Support Office, National Cancer Center Hospital, Tokyo, Japan. FAU - Nogami, Yuki AU - Nogami Y AD - Department of Pediatric Oncology, National Cancer Center Hospital, Tokyo, Japan. FAU - Iwata, Shintaro AU - Iwata S AD - Department of Musculoskeletal Oncology and Rehabilitation, National Cancer Center Hospital, Tokyo, Japan. FAU - Suzuki, Tatsuya AU - Suzuki T AD - Department of Hematology , National Cancer Center Hospital, Tokyo, Japan. FAU - Satomi, Eriko AU - Satomi E AD - Department of Palliative Medicine, National Cancer Center Hospital, Tokyo, Japan. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Oncol JT - Frontiers in oncology JID - 101568867 PMC - PMC13713312 OTO - NOTNLM OT - adolescent and young adult OT - cancer survivorship OT - long-term follow-up OT - survivorship care OT - telemedicine COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/14 CRDT- 2026/10/29 04:59 PHST- 2026/08/25 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:59 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fonc.2026.1977660 [doi] PST - epublish SO - Front Oncol. 2026 Oct 14;16:1977660. doi: 10.3389/fonc.2026.1977660. eCollection 2026. PMID- 42952539 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2234-943X (Print) IS - 2234-943X (Electronic) IS - 2234-943X (Linking) VI - 16 DP - 2026 TI - Multiparametric MRI radiomics for preoperative prediction of tumor budding grade in rectal cancer. PG - 1984365 LID - 10.3389/fonc.2026.1984365 [doi] LID - 1984365 AB - BACKGROUND: Tumor budding (TB) is a histopathological marker of tumor aggressiveness and an established prognostic factor in colorectal cancer. In rectal cancer, however, TB is usually assessed on biopsy or resection material, and a reliable result may not be available when preoperative management is being considered. PURPOSE: To develop and internally validate a radiomics model for preoperative discrimination of low-grade versus intermediate/high-grade TB in patients with rectal cancer who proceed to upfront surgery without neoadjuvant treatment. The primary model was selected according to the highest repeated cross-validation AUC, with alternative feature sets and algorithms evaluated exploratorily. METHODS: This retrospective study included 139 patients with pathologically confirmed rectal cancer who had received no neoadjuvant treatment. TB was assessed on postoperative resection specimens. T1WI, T2WI, arterial-phase contrast-enhanced T1WI, and DWI were analyzed. Radiomics features were extracted from the tumor core, a combined tumor-plus-2-mm expansion, and an isolated external 2-mm ring. Seven feature sets and four machine-learning algorithms were evaluated; the primary model was selected according to the highest repeated cross-validation AUC. Feature filtering, imputation, selection, and scaling were performed within the training portion of every split. Model performance was estimated using stratified five-fold cross-validation repeated five times and patient-level out-of-fold (OOF) probabilities. RESULTS: The primary core-ROI Elastic Net model achieved a repeated cross-validation AUC of 0.825 (range 0.809-0.839 across repetitions), with PR-AUC 0.781, sensitivity 0.604, specificity 0.851, and Brier score 0.173. Its patient-level mean OOF AUC was 0.829 (2,000 paired bootstrap replicates; 95% CI, 0.755-0.896). At a probability threshold of 0.50, patient-level sensitivity was 0.607 and specificity was 0.867. In conditional paired bootstrap comparisons, the core model showed higher AUCs than the clinical, peritumoral-ring, clinical-plus-expanded, clinical-plus-core, and clinical-plus-core-plus-ring models (BH-adjusted P = 0.004-0.005). The difference versus the expanded ROI was smaller and did not reach statistical significance after adjustment (P = 0.052). CONCLUSION: Multiparametric MRI radiomics features from the tumor core showed promising internal discrimination of low-grade versus intermediate/high-grade TB among patients who underwent upfront surgery without neoadjuvant treatment. The model may support preoperative TB risk stratification in this population, but external validation is required before clinical use. CI - Copyright (c) 2026 Chen, Zhou, Wu, Zhang, He, Pan, Xia, Li, Hou, Shen and Chen. FAU - Chen, Peng AU - Chen P AD - Department of Radiology, Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University, Huzhou, China. FAU - Zhou, Yanyan AU - Zhou Y AD - Department of Radiology, Changxing Hospital of Traditional Chinese Medicine, Huzhou, China. FAU - Wu, Dan AU - Wu D AD - Department of Radiology, Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University, Huzhou, China. FAU - Zhang, Chengmeng AU - Zhang C AD - Department of Radiology, Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University, Huzhou, China. FAU - He, Xiangyi AU - He X AD - Department of Pathology, Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University, Huzhou, China. FAU - Pan, Ru AU - Pan R AD - Department of Pathology, Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University, Huzhou, China. FAU - Xia, Dong AU - Xia D AD - Department of Radiology, Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University, Huzhou, China. FAU - Li, Jinge AU - Li J AD - School of Medicine and Nursing, Huzhou Normal University, Huzhou, China. FAU - Hou, Jue AU - Hou J AD - School of Medicine and Nursing, Huzhou Normal University, Huzhou, China. FAU - Shen, Jian AU - Shen J AD - Department of Radiology, Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University, Huzhou, China. FAU - Chen, Hao AU - Chen H AD - Department of Radiology, Huzhou Central Hospital, Fifth School of Clinical Medicine of Zhejiang Chinese Medical University, Huzhou, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Oncol JT - Frontiers in oncology JID - 101568867 PMC - PMC13713355 OTO - NOTNLM OT - explainable artificial intelligence OT - machine learning OT - magnetic resonance imaging OT - radiomics OT - rectal cancer OT - tumor budding COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/14 CRDT- 2026/10/29 04:58 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:58 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fonc.2026.1984365 [doi] PST - epublish SO - Front Oncol. 2026 Oct 14;16:1984365. doi: 10.3389/fonc.2026.1984365. eCollection 2026. PMID- 42952536 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-1078 (Print) IS - 1664-1078 (Electronic) IS - 1664-1078 (Linking) VI - 17 DP - 2026 TI - The impact of hospital ethical climate on ICU nurses' ethical behavior: mediated by moral courage. PG - 1936074 LID - 10.3389/fpsyg.2026.1936074 [doi] LID - 1936074 AB - OBJECTIVE: This study purpose to explore the influence of hospital ethical climate on moral courage and ethical behavior of ICU nurses and to examine the mediating role of moral courage. METHODS: Nurses were selected from the intensive care units (ICUs) of three Grade-A tertiary hospitals in Henan Province by convenience sampling and data collated from General Information Questionnaire, Hospital Ethical Climate Scale, Nurses' Moral Courage Scale and Ethical Behavior Scale for Nurses-Revised. Pearson correlation analysis was used to evaluate associations among the three variables and structural equation modeling to test the influence of hospital ethical climate and ICU nurses' moral courage on ethical behavior. RESULTS: A total of 243 questionnaires were distributed, and 222 valid responses were returned, yielding an effective response rate of 91.4%. Correlation analysis revealed significant positive pairwise correlations among hospital ethical climate, ICU nurses' moral courage, and ethical behavior (r = 0.740 ~ 0.814, p < 0.01). Further mediation analysis indicated than there was a positive association between hospital ethical climate and ICU nurses' ethical behavior (t = 17.032, p < 0.01), moral courage was also positively associated with ethical behavior (t = 3.877, p < 0.01). Moreover, moral courage partially mediated the relationship between hospital ethical climate and ethical behavior, with the mediating effect accounting for 32.1% of the total effect. CONCLUSION: Moral courage plays a mediating role between perceived hospital ethical climate and ethical behavior in ICU nurses. Accordingly, nursing managers are advised to implement multiple strategies to promote a supportive ethical climate and nurture moral courage, so as to improve ethical conduct among ICU nurses, guarantee patient safety and care quality, and maintain their professional competency and psychological well-being. CI - Copyright (c) 2026 Zhao, Zhu, Zheng and Wang. FAU - Zhao, Zhiling AU - Zhao Z AD - Coronary Care Unit, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan, China. FAU - Zhu, Keke AU - Zhu K AD - Coronary Care Unit, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan, China. FAU - Zheng, Fangqi AU - Zheng F AD - Coronary Care Unit, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan, China. FAU - Wang, He AU - Wang H AD - Coronary Care Unit, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan, China. AD - Nursing Department, Henan University of Chinese Medicine, Zhengzhou, Henan, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Psychol JT - Frontiers in psychology JID - 101550902 PMC - PMC13713357 OTO - NOTNLM OT - ICU nurses OT - ethical behavior OT - hospital ethical climate OT - mediating effect OT - moral courage COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/14 CRDT- 2026/10/29 04:58 PHST- 2026/07/13 00:00 [received] PHST- 2026/08/19 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:58 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fpsyg.2026.1936074 [doi] PST - epublish SO - Front Psychol. 2026 Oct 14;17:1936074. doi: 10.3389/fpsyg.2026.1936074. eCollection 2026. PMID- 42952534 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 2234-943X (Print) IS - 2234-943X (Electronic) IS - 2234-943X (Linking) VI - 16 DP - 2026 TI - IVIM-based habitat imaging for non-invasive assessment of Ki-67 proliferation status in cervical cancer. PG - 1885498 LID - 10.3389/fonc.2026.1885498 [doi] LID - 1885498 AB - OBJECTIVE: This study aimed to evaluate whether voxel-level IVIM-based habitat analysis supports exploratory pretreatment stratification of Ki-67 proliferation status in cervical cancer. MATERIALS AND METHODS: Between January 2019 and June 2025, medical records of 149 patients with histopathologically confirmed cervical cancer were initially screened. Before image-quality assessment, 49 were excluded because IVIM MRI had been performed after chemoradiotherapy (n = 5), cervical biopsy sampling (n = 21), or lesion excision (n = 10), or because no pathological Ki-67 immunohistochemistry result was available (n = 13). Of the remaining 100 patients, 17 were excluded because of severe image artefacts (n = 11) or lesions too small for reliable ROI delineation (n = 6), leaving 83 patients for analysis. Patients were stratified into high Ki-67 (Ki-67 >70%, n = 38) and low/equal Ki-67 (Ki-67 10%, red blood cell count <4x10(12)/L, mean platelet volume <8 fL, and plateletcrit <0.1%.In the independent external validation (n=488), the LightGBM model remained stable and reliable, with an AUC of 0.940 (95% CI: 0.920-0.959), sensitivity of 0.818, specificity of 0.888, accuracy of 85.2%, and favorable calibration and clinical net benefit. CONCLUSION: The LightGBM prediction model developed in this study can enable early identification of high-risk patients, providing a reference for healthcare providers to implement early interventions. CI - Copyright (c) 2026 Fan, Wu, Cheng, Tang, Huang and Chen. FAU - Fan, Hao AU - Fan H AD - Department of Pharmacy, Northeast Yunnan Central Hospital, Zhaotong, China. FAU - Wu, Xuejiao AU - Wu X AD - Department of Pharmacy, Yiliang County People's Hospital, Zhaotong, China. FAU - Cheng, Zhenyu AU - Cheng Z AD - Department of Pharmacy, Northeast Yunnan Central Hospital, Zhaotong, China. FAU - Tang, Xiubai AU - Tang X AD - Department of Pharmacy, Northeast Yunnan Central Hospital, Zhaotong, China. FAU - Huang, Hui AU - Huang H AD - Department of Pharmacy, Northeast Yunnan Central Hospital, Zhaotong, China. FAU - Chen, Keyan AU - Chen K AD - Department of Pharmacy, Northeast Yunnan Central Hospital, Zhaotong, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Oncol JT - Frontiers in oncology JID - 101568867 PMC - PMC13713280 OTO - NOTNLM OT - SHAP analysis OT - chemotherapy-induced myelosuppression OT - machine learning OT - prediction model OT - risk factors COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/14 CRDT- 2026/10/29 04:56 PHST- 2026/01/04 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:56 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fonc.2026.1780678 [doi] PST - epublish SO - Front Oncol. 2026 Oct 14;16:1780678. doi: 10.3389/fonc.2026.1780678. eCollection 2026. PMID- 42952512 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-042X (Print) IS - 1664-042X (Electronic) IS - 1664-042X (Linking) VI - 17 DP - 2026 TI - Acute effects of bench press conditioning performed with different velocity-loss thresholds on punch performance in elite adolescent boxers. PG - 1973551 LID - 10.3389/fphys.2026.1973551 [doi] LID - 1973551 AB - BACKGROUND: Velocity-loss (VL) thresholds may help regulate the balance between potentiation and fatigue during post-activation performance enhancement protocols; however, their effects on boxing-specific performance remain unclear. This study investigated the acute effects of bench press conditioning regulated by different VL thresholds on straight-punch performance in adolescent boxers. METHODS: Eighteen male boxers (17.6 +/- 0.7 years) completed four conditions in a randomized, counterbalanced crossover design: an active control condition (CON; two sets of 10 bench press repetitions using a habitual warm-up load below 30% one-repetition maximum [1RM]) and three VL conditions consisting of two sets of bench press at 85% 1RM terminated at 10% (VL10%), 20% (VL20%), or 30% (VL30%) velocity loss. Lead- and rear-hand peak punch velocity (PPV), peak punch force (PPF), and an estimated peak punch-power index (PPP) were assessed at baseline and 4, 8, 12, and 16 min after conditioning. Outcomes were analyzed using linear mixed-effects models, followed by Dunnett-adjusted comparisons of each VL condition with CON. Additional Tukey-adjusted pairwise comparisons were performed among VL10%, VL20%, and VL30%. RESULTS: Significant condition x time interactions were observed for rear-hand PPV, lead- and rear-hand PPF, and lead- and rear-hand PPP (all p /=50% of any brainstem section on any axial or coronal slice of MRI was considered HALF-IT positive, whereas < 50% was considered HALF-IT negative. The primary efficacy outcome was a favorable functional outcome, defined as a modified Rankin Scale (mRS) score of 0-3 at 90 days; functional independence (mRS 0-2) was analyzed as a secondary outcome. Reperfusion was graded with the modified Thrombolysis in Cerebral Infarction (mTICI) scale. Primary safety endpoints included symptomatic intracranial hemorrhage at 24 h and all-cause mortality at 90 days. Multivariable logistic regression was performed to identify independent predictors of the primary efficacy outcome. RESULTS: 37 patients (23 HALF-IT negative; 14 HALF-IT positive) met the inclusion criteria. The rate of 90-day mRS 0-3 was significantly lower in the HALF-IT positive compared with HALF-IT negative group [14.3% vs. 68.2%, OR 0.08 (0.01-0.45), p = 0.004]. HALF-IT positive status remained associated with a lower rate of 90-day mRS 0-3 after adjusting for age, initial NIHSS, onset-to-reperfusion time, intravenous thrombolysis administration complete reperfusion (final mTICI 3) [adjusted OR 0.02, 95% CI 0-0.84, p = 0.02]. Safety events were numerically higher in the HALF-IT positive group but the difference did not reach statistical significance. The two groups differed substantially at baseline in age, presenting NIHSS, and intravenous thrombolysis exposure, whereas stroke etiology (p = 0.28) and level of basilar occlusion (p = 0.63) were similarly distributed. CONCLUSION: In this small, retrospective, hypothesis-generating cohort, HALF-IT positivity was associated with worse 90-day functional outcomes after EVT for BAO. Further prospective validation in larger cohorts is required before the score can inform treatment decisions. CI - Copyright (c) 2026 Jones, Tariq, Ashouri, Chaudhari, AlMajali, Ezzeldin, Kareem, Awwad, Wong, Molaie, Sanz Cuesta, Saver, Limaye and Zaidat. FAU - Jones, Ann Marie AU - Jones AM AD - Department of Vascular and Endovascular Neurology, Indiana University School of Medicine, Indianapolis, IN, United States. FAU - Tariq, Zain AU - Tariq Z AD - Department of Neuroscience, Bon Secours Mercy Health St. Vincent Hospital, Toledo, OH, United States. FAU - Ashouri, Yazan AU - Ashouri Y AD - Department of Neuroscience, Bon Secours Mercy Health St. Vincent Hospital, Toledo, OH, United States. FAU - Chaudhari, Amit AU - Chaudhari A AD - Department of Neuroscience, Bon Secours Mercy Health St. Vincent Hospital, Toledo, OH, United States. FAU - AlMajali, Mohammad AU - AlMajali M AD - Department of Neuroendovascular Surgery, Ochsner Clinic Foundation Lafayette General Hospital, Lafayette, LA, United States. FAU - Ezzeldin, Mohamad AU - Ezzeldin M AD - Department of Neuroendovascular Surgery, HCA Houston Healthcare Kingwood, Kingwood, TX, United States. FAU - Kareem, Mustafa Abdul AU - Kareem MA AD - Department of Neuroscience, Bon Secours Mercy Health St. Vincent Hospital, Toledo, OH, United States. FAU - Awwad, Ahmad R AU - Awwad AR AD - Department of Neuroscience, Bon Secours Mercy Health St. Vincent Hospital, Toledo, OH, United States. FAU - Wong, Gregory AU - Wong G AD - Department of Vascular and Endovascular Neurology, University of California, Los Angeles, CA, United States. FAU - Molaie, Amir M AU - Molaie AM AD - Department of Vascular and Endovascular Neurology, University of California, Los Angeles, CA, United States. FAU - Sanz Cuesta, Borja AU - Sanz Cuesta B AD - Department of Vascular and Endovascular Neurology, University of California, Los Angeles, CA, United States. FAU - Saver, Jeffrey L AU - Saver JL AD - Department of Vascular and Endovascular Neurology, University of California, Los Angeles, CA, United States. FAU - Limaye, Kaustubh AU - Limaye K AD - Department of Vascular and Endovascular Neurology, Indiana University School of Medicine, Indianapolis, IN, United States. FAU - Zaidat, Osama O AU - Zaidat OO AD - Department of Neuroscience, Bon Secours Mercy Health St. Vincent Hospital, Toledo, OH, United States. LA - eng PT - Journal Article PT - Multicenter Study DEP - 20261014 PL - Switzerland TA - Front Neurol JT - Frontiers in neurology JID - 101546899 SB - IM MH - Humans MH - Retrospective Studies MH - *Vertebrobasilar Insufficiency/surgery MH - *Thrombectomy/methods MH - Female MH - Male MH - Aged MH - *Triage/methods MH - Endovascular Procedures MH - Middle Aged MH - *Severity of Illness Index MH - Aged, 80 and over MH - Treatment Outcome MH - Basilar Artery MH - *Outcome Assessment, Health Care PMC - PMC13713328 OTO - NOTNLM OT - basilar artery occlusion OT - endovascular thrombectomy OT - ischemic stroke OT - neuroimaging OT - triaging tool COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The authors KL and OZ declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 15:20 PMCR- 2026/10/14 CRDT- 2026/10/29 04:56 PHST- 2026/06/21 00:00 [received] PHST- 2026/09/04 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:56 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fneur.2026.1915436 [doi] PST - epublish SO - Front Neurol. 2026 Oct 14;17:1915436. doi: 10.3389/fneur.2026.1915436. eCollection 2026. PMID- 42952502 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-2295 (Electronic) IS - 1664-2295 (Linking) VI - 17 DP - 2026 TI - Association of endovascular treatment versus intravenous thrombolysis in mild stroke across occlusion subgroups. PG - 1878069 LID - 10.3389/fneur.2026.1878069 [doi] LID - 1878069 AB - BACKGROUND: The benefit of endovascular treatment (EVT) vs. intravenous thrombolysis (IVT) is uncertain in minor stroke patients (National Institutes of Health Stroke Scale [NIHSS] /= 25%, calculated from the peak slow-phase velocities (SPVs) of all four irrigations using a uniform formula, served as the primary reference standard. In the derivation cohort, 200 predefined combinations of warm monothermal caloric asymmetry (MCA), the lower of the two warm SPVs (WarmMin), and spontaneous nystagmus SPV were evaluated, with sensitivity >/=97.5% as the primary constraint. The selected rule was then applied unchanged to the temporal validation cohort. RESULTS: Among 2,316 patients in the validation cohort, 846 (36.5%) had CP >/= 25%. The AUC was 0.888 (95% CI, 0.873-0.902) for warm monothermal testing and 0.777 (95% CI, 0.756-0.798) for cold monothermal testing (AUC difference, 0.111; 95% CI, 0.084-0.136; DeLong p < 0.001). Of the 200 candidate rules, 99 achieved sensitivity >/=97.5%. The final candidate rule required |MCA_W| < 17.5%, WarmMin >11 degrees /s, and spontaneous nystagmus SPV < 4 degrees /s. In the validation cohort, this rule yielded a sensitivity of 98.3% (95% CI, 97.2-99.1%), specificity of 36.3% (95% CI, 33.8-38.8%), and negative predictive value of 97.4% (95% CI, 95.7-98.6%). Overall, 547 patients (23.6%) met the candidate-stop criteria, including 14 false negatives with CP >/= 25%. Per 1,000 patients, the rule was estimated to avoid 472.4 subsequent cold irrigations while missing 6.0 cases with CP >/= 25%. No false negatives with CP >/= 40% were observed. CONCLUSION: Warm monothermal testing showed better overall discrimination than cold monothermal testing. A composite rule incorporating MCA, minimum warm response strength, and spontaneous nystagmus SPV identified a subset of low-risk patients, but still produced a small number of CP-positive false negatives. The rule should not replace complete bithermal testing and requires independent prospective validation. CI - Copyright (c) 2026 Ma, Shen, Zhang, Wang, Wang, Jin, Yang and Chen. FAU - Ma, Xiaobao AU - Ma X AD - Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Shen, Jiali AU - Shen J AD - Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Zhang, Qin AU - Zhang Q AD - Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Wang, Lu AU - Wang L AD - Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Wang, Wei AU - Wang W AD - Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Jin, Yulian AU - Jin Y AD - Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Yang, Jun AU - Yang J AD - Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Chen, Jianyong AU - Chen J AD - Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. LA - eng PT - Journal Article PT - Validation Study DEP - 20261014 PL - Switzerland TA - Front Neurol JT - Frontiers in neurology JID - 101546899 SB - IM MH - Humans MH - *Caloric Tests/methods/standards MH - Retrospective Studies MH - *Triage/methods/standards MH - Female MH - *Paresis/diagnosis MH - Cold Temperature MH - Sensitivity and Specificity MH - Hot Temperature PMC - PMC13713255 OTO - NOTNLM OT - caloric testing OT - canal paresis OT - diagnostic accuracy OT - internal temporal validation OT - monothermal testing OT - spontaneous nystagmus COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 15:20 PMCR- 2026/10/14 CRDT- 2026/10/29 04:53 PHST- 2026/07/30 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:53 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fneur.2026.1953594 [doi] PST - epublish SO - Front Neurol. 2026 Oct 14;17:1953594. doi: 10.3389/fneur.2026.1953594. eCollection 2026. PMID- 42952475 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1664-2392 (Print) IS - 1664-2392 (Electronic) IS - 1664-2392 (Linking) VI - 17 DP - 2026 TI - The diagnostic journey of fibrodysplasia ossificans progressiva: evidence from a national cohort of 114 patients. PG - 1941326 LID - 10.3389/fendo.2026.1941326 [doi] LID - 1941326 AB - BACKGROUND: Fibrodysplasia ossificans progressiva (FOP) is an ultra-rare genetic disorder caused by gain-of-function variants in ACVR1, characterized by congenital great-toe malformations, recurrent inflammatory soft-tissue swellings, and progressive heterotopic ossification (HO). Early lesions may resemble neoplastic, inflammatory, infectious, or trauma-related conditions, whereas invasive procedures can trigger additional HO. METHODS: We performed a cross-sectional analysis of 114 patients with molecularly confirmed FOP enrolled in the National Rare Diseases Registry System of China. The diagnostic journey was reconstructed to evaluate diagnostic delay, misdiagnosis, missed diagnostic opportunities, and patient-reported outcomes after high-risk procedures. RESULTS: Patients were recruited from 23 provinces or regions. Diagnostic delay exceeded 3 years in 34.5% patients and 10 years in 9.7% patients. FOP was not considered at the first hospital visit in 85 of 114 patients (74.6%), and 72 of 114 (63.2%) reported misdiagnosis. Among misdiagnosed patients, tumor-related diagnoses were most common (36/72, 50.0%), followed by orthopedic or congenital (13/72, 18.1%) and inflammatory or infectious diagnoses (12/72, 16.7%). 102 of 114 patients (89.5%) reported congenital great-toe malformation. Biopsy was reported by 51 patients (44.7%), of whom 41 (80.4%) reported subsequent worsening; 22 of 25 patients (88.0%) who underwent HO excision reported recurrence or new lesions at the surgical site. CONCLUSION: Diagnostic errors remained common in FOP. Biopsy and HO excision were also frequently reported in FOP patients and were often followed by patient-reported worsening or recurrence. These findings provide a rationale for prospective evaluation of an early-recognition pathway emphasizing examination of the great toes, avoidance of invasive procedures when FOP is suspected, ACVR1 testing, and specialist referral. CI - Copyright (c) 2026 Cui, Ouyang, Guo, Cai, Pang, Chi, Jiajue, Li, Liu, Jiang, Wang, Li, Xing, Xu and Xia. FAU - Cui, Lijia AU - Cui L AD - Department of Endocrinology, National Health Commission (NHC) Key Laboratory of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. AD - State Key Laboratory of Complex, Severe, and Rare Diseases, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Ouyang, Quanshi AU - Ouyang Q AD - Department of Endocrinology, National Health Commission (NHC) Key Laboratory of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. AD - State Key Laboratory of Complex, Severe, and Rare Diseases, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Guo, Jian AU - Guo J AD - State Key Laboratory of Complex, Severe, and Rare Diseases, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Cai, Wanping AU - Cai W AD - Department of Endocrinology, National Health Commission (NHC) Key Laboratory of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. AD - State Key Laboratory of Complex, Severe, and Rare Diseases, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Pang, Qianqian AU - Pang Q AD - Department of Endocrinology, National Health Commission (NHC) Key Laboratory of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. AD - State Key Laboratory of Complex, Severe, and Rare Diseases, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Chi, Yue AU - Chi Y AD - Department of Endocrinology, National Health Commission (NHC) Key Laboratory of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. AD - State Key Laboratory of Complex, Severe, and Rare Diseases, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Jiajue, Ruizhi AU - Jiajue R AD - Department of Endocrinology, National Health Commission (NHC) Key Laboratory of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. AD - State Key Laboratory of Complex, Severe, and Rare Diseases, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Li, Xiang AU - Li X AD - Department of Endocrinology, National Health Commission (NHC) Key Laboratory of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. AD - State Key Laboratory of Complex, Severe, and Rare Diseases, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Liu, Wei AU - Liu W AD - Department of Endocrinology, National Health Commission (NHC) Key Laboratory of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. AD - State Key Laboratory of Complex, Severe, and Rare Diseases, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Jiang, Yan AU - Jiang Y AD - Department of Endocrinology, National Health Commission (NHC) Key Laboratory of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. AD - State Key Laboratory of Complex, Severe, and Rare Diseases, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Wang, Ou AU - Wang O AD - Department of Endocrinology, National Health Commission (NHC) Key Laboratory of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. AD - State Key Laboratory of Complex, Severe, and Rare Diseases, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Li, Mei AU - Li M AD - Department of Endocrinology, National Health Commission (NHC) Key Laboratory of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. AD - State Key Laboratory of Complex, Severe, and Rare Diseases, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Xing, Xiaoping AU - Xing X AD - Department of Endocrinology, National Health Commission (NHC) Key Laboratory of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. AD - State Key Laboratory of Complex, Severe, and Rare Diseases, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. FAU - Xu, Qiming AU - Xu Q AD - Department of Orthopedic Oncology, Beijing Jishuitan Hospital, Capital Medical University, Beijing, China. FAU - Xia, Weibo AU - Xia W AD - Department of Endocrinology, National Health Commission (NHC) Key Laboratory of Endocrinology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. AD - State Key Laboratory of Complex, Severe, and Rare Diseases, Institute of Clinical Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Endocrinol (Lausanne) JT - Frontiers in endocrinology JID - 101555782 RN - EC 2.7.11.30 (ACVR1 protein, human) RN - EC 2.7.11.30 (Activin Receptors, Type I) SB - IM MH - Humans MH - *Myositis Ossificans/diagnosis/genetics/epidemiology MH - Female MH - Male MH - Cross-Sectional Studies MH - Child MH - Adult MH - Adolescent MH - China/epidemiology MH - Child, Preschool MH - Delayed Diagnosis/statistics & numerical data MH - *Diagnostic Errors/statistics & numerical data MH - Young Adult MH - Registries MH - Activin Receptors, Type I/genetics MH - Infant MH - Cohort Studies PMC - PMC13713263 OTO - NOTNLM OT - Acvr1 OT - diagnostic delay OT - fibrodysplasia ossificans progressiva OT - iatrogenic harm OT - misdiagnosis OT - missed diagnosis COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 15:20 PMCR- 2026/10/14 CRDT- 2026/10/29 04:50 PHST- 2026/07/18 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/29 15:20 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:50 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 10.3389/fendo.2026.1941326 [doi] PST - epublish SO - Front Endocrinol (Lausanne). 2026 Oct 14;17:1941326. doi: 10.3389/fendo.2026.1941326. eCollection 2026. PMID- 42952461 OWN - NLM STAT- Publisher LR - 20261029 IS - 1521-3927 (Electronic) IS - 1022-1336 (Linking) DP - 2026 Oct 29 TI - Post-Synthetic Thioesterification of Carboxyl-Functionalized HCP for Enhanced CO(2) Capture and Selectivity. LID - e70463 [pii] LID - 10.1002/marc.70463 [doi] AB - Post-synthetic modification (PSM) is an effective route to improve the CO(2) capture and CO(2)/N(2) selectivity of hyper-cross-linked polymers (HCPs) by introducing tailored adsorption sites. A key challenge, however, is achieving these improvements without substantially compromising porosity. Here, we report a simple PSM strategy that incorporates multiple CO(2)-philic functionalities through a condensation reaction between a carboxyl-functionalized HCP (HCP-TRIP) and thiourea-formaldehyde resin, yielding a thioester-functionalized material (HCP-TRIP-P). The modification largely preserves the porous framework, with only a minor reduction in Brunauer-Emmett-Teller (BET) surface area from 843 to 794 m(2) g(-1). In parallel, CO(2) uptake increases by 43%, from 15.4 wt.% to 22.0 wt.% at 273 K and 1 bar. The CO(2)/N(2) selectivity is also markedly enhanced, rising from 31 to 82 as estimated by the Henry initial slope method, and from 25 to 53 based on ideal adsorbed solution theory (IAST) predictions for a CO(2)/N(2) (15/85 v/v) gas mixture at 298 K and 1 bar. Dynamic breakthrough experiments confirm effective CO(2)/N(2) separation under dry and 50% RH conditions; HCP-TRIP-P retains > 99% adsorption capacity over five cycles at 273 K. This work demonstrates a facile PSM route to simultaneously enhance CO(2) adsorption and CO(2)/N(2) selectivity while maintaining the intrinsic porosity of the HCPs. CI - (c) 2026 Wiley-VCH GmbH. FAU - Alemin, Yahya AU - Alemin Y AD - Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, China. FAU - Qin, Haojie AU - Qin H AD - Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, China. FAU - Hu, Jiarui AU - Hu J AD - Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, China. FAU - Xie, Peixuan AU - Xie P AD - Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, China. FAU - Wang, Xiaoyan AU - Wang X AUID- ORCID: 0000-0003-1876-532X AD - Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, China. FAU - Gao, Hui AU - Gao H AUID- ORCID: 0000-0003-4415-6413 AD - Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, China. FAU - Tan, Bien AU - Tan B AUID- ORCID: 0000-0001-7181-347X AD - Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan, China. LA - eng GR - 22475076/National Natural Science Foundation of China/ GR - 52403268/National Natural Science Foundation of China/ GR - 2024AFB042/Natural Science Foundation of Hubei Province/ GR - 2025011202030399/Key R&D Project of Wuhan City/ GR - 202504060102022/Natural Science Foundation of Wuhan City/ GR - B21003/Innovation and Talent Recruitment Base of New Energy Chemistry and Device/ GR - 2026JYBKF04/Open Research Fund of the Key Laboratory of Material Chemistry for Energy Conversion and Storage (HUST), Ministry of Education/ PT - Journal Article DEP - 20261029 PL - Germany TA - Macromol Rapid Commun JT - Macromolecular rapid communications JID - 9888239 SB - IM OTO - NOTNLM OT - CO2/N2 selectivity OT - hyper-cross-linked polymers OT - porosity engineering OT - post-synthetic modification OT - selective CO2 adsorption EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 04:44 PHST- 2026/10/13 00:00 [revised] PHST- 2026/06/12 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:44 [entrez] AID - e70463 [pii] AID - 10.1002/marc.70463 [doi] PST - aheadofprint SO - Macromol Rapid Commun. 2026 Oct 29:e70463. doi: 10.1002/marc.70463. PMID- 42952460 OWN - NLM STAT- Publisher LR - 20261029 IS - 1592-8721 (Electronic) IS - 0390-6078 (Linking) DP - 2026 Oct 29 TI - Platelet transfusion biology: from hemostatic support to inflammatory and immune signaling. LID - 10.3324/haematol.2026.301566 [doi] AB - Platelet transfusion remains indispensable for the prevention and treatment of bleeding in hematology, oncology, transplantation, critical care, surgery and trauma. Yet the biological impact of a platelet concentrate extends well beyond numerical correction of thrombocytopenia. Platelets are anucleate but metabolically active immune cells that express pattern-recognition receptors, release cytokines, chemokines, lipid mediators, extracellular vesicles, mitochondria-derived material and damage-associated molecular patterns; and interact with leukocytes, endothelial cells and complement. During collection, processing and storage, platelet concentrates acquire a distinct productspecific phenotype shaped by donor biology, residual plasma, additive solutions, leukoreduction, pathogen reduction, temperature and storage time. These variables affect not only post-transfusion recovery and hemostatic function, but also inflammatory signaling and immune related complications. The recipient is equally important: sepsis, chemotherapy, malignancy, neonatal immune development, endothelial activation and prior alloimmunization can determine whether transfused platelets restore hemostasis, amplify inflammation, or trigger refractoriness. This review explores platelet transfusion through the lens of a biological triad formed by the donor, the platelet product, and the recipient. We focus on how platelet immune receptors, storage-induced inflammatory remodeling, soluble biological response modifiers, extracellular vesicles and complement shape the interactions of transfused platelets with recipient leukocytes and endothelium. Alloimmunization and refractoriness are considered more briefly as recipient-dependent modifiers of platelet recovery and transfusion efficacy. The central challenge is no longer to define a single optimal platelet product, but to engineer and select the right platelet product for the right patient, at the right moment. FAU - Hamzeh-Cognasse, Hind AU - Hamzeh-Cognasse H AD - INSERM, U 1059 SAINBIOSE, Universite Jean Monnet, Saint-Etienne. FAU - Maitre, Blandine AU - Maitre B AD - Universite de Strasbourg, INSERM, Etablissement Francais du Sang (EFS) Grand Est, BPPS UMR_S1255, Federation de Medecine Translationnelle de Strasbourg (FMTS), Strasbourg. FAU - Lambert, Michele P AU - Lambert MP AD - Division of Hematology, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania. FAU - Cognasse, Fabrice AU - Cognasse F AD - INSERM, U 1059 SAINBIOSE, Universite Jean Monnet, Saint-Etienne, France; Etablissement Francais du Sang Auvergne-Rhone-Alpes, Saint-Etienne. fabrice.cognasse@efs.sante.fr. LA - eng PT - Journal Article DEP - 20261029 PL - Italy TA - Haematologica JT - Haematologica JID - 0417435 SB - IM EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 04:44 PHST- 2026/07/06 00:00 [received] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:44 [entrez] AID - 10.3324/haematol.2026.301566 [doi] PST - aheadofprint SO - Haematologica. 2026 Oct 29. doi: 10.3324/haematol.2026.301566. PMID- 42952457 OWN - NLM STAT- Publisher LR - 20261029 IS - 2328-9503 (Electronic) IS - 2328-9503 (Linking) DP - 2026 Oct 29 TI - Acute Endocrine Alterations After Traumatic Brain Injury and 6-Month Functional Outcome. LID - 10.1002/acn3.70570 [doi] AB - OBJECTIVE: Acute multi-axis endocrine alterations after traumatic brain injury (TBI) remain incompletely characterized. This study aimed to examine their temporal patterns and explore their associations with 6-month functional outcome and early inflammatory markers. METHODS: We conducted a single-center prospective observational cohort study of hospitalized adults with traumatic brain injury. Among 130 enrolled patients, 125 with complete 6-month Glasgow Outcome Scale-Extended follow-up were included. Blood samples were collected on Day 1, 7, and 14 after injury to assess hypothalamic-pituitary-adrenal, thyroid, gonadal, and growth hormone axes. Day-1 inflammatory markers were measured. Associations of individual biomarkers with unfavorable 6-month outcome were evaluated using separate Firth penalized logistic regression models adjusted for admission Glasgow Coma Scale (GCS). RESULTS: Serum cortisol levels at 08:00 and 16:00 decreased significantly over time (Kendall's W = 0.205 [95% CI 0.085-0.388] and 0.239 [95% CI 0.126-0.408], respectively; both p < 0.001), whereas triiodothyronine, free triiodothyronine, and thyroid-stimulating hormone increased during the acute phase. Patients with unfavorable 6-month outcome had higher day-1 interleukin-6, neutrophil count, and procalcitonin levels and lower day-1 triiodothyronine and free triiodothyronine levels than those with favorable outcome. After adjustment for admission GCS in separate Firth models, interleukin-6, white blood cell count, neutrophil count, procalcitonin, day-1 triiodothyronine, and day-1 free triiodothyronine were not significantly associated with unfavorable 6-month outcome. INTERPRETATION: Endocrine alterations after acute TBI were heterogeneous across injury severity groups and time points. Although selected day-1 inflammatory and thyroid-axis measures differed between outcome groups, their associations with unfavorable 6-month functional outcome were not statistically significant after adjustment for admission GCS. These outcome-related findings require validation in larger cohorts. CI - (c) 2026 The Author(s). Annals of Clinical and Translational Neurology published by Wiley Periodicals LLC on behalf of American Neurological Association. FAU - Xu, Biao AU - Xu B AD - The First Clinical Medical College of Ningxia Medical University, Yinchuan, Ningxia, China. AD - Key Laboratory of Ningxia Stem Cell and Regenerative Medicine, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, China. FAU - Guo, Peng AU - Guo P AD - The 904th Hospital of the Joint Logistic Support Force of PLA, Wuxi, Jiangsu, China. FAU - Pan, Rui AU - Pan R AD - The First Clinical Medical College of Ningxia Medical University, Yinchuan, Ningxia, China. AD - Key Laboratory of Ningxia Stem Cell and Regenerative Medicine, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, China. FAU - Wang, Zhenghao AU - Wang Z AD - The First Clinical Medical College of Ningxia Medical University, Yinchuan, Ningxia, China. AD - Key Laboratory of Ningxia Stem Cell and Regenerative Medicine, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, China. FAU - Zhou, Jingchao AU - Zhou J AD - The First Clinical Medical College of Ningxia Medical University, Yinchuan, Ningxia, China. AD - Department of Neurosurgery, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China. FAU - Zhang, Pengcheng AU - Zhang P AD - The First Clinical Medical College of Ningxia Medical University, Yinchuan, Ningxia, China. AD - Key Laboratory of Ningxia Stem Cell and Regenerative Medicine, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, China. FAU - Zhang, Xingzhe AU - Zhang X AD - The First Clinical Medical College of Ningxia Medical University, Yinchuan, Ningxia, China. AD - Department of Neurosurgery, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China. FAU - Zhang, Qinghua AU - Zhang Q AD - Department of Neurosurgery, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China. FAU - Ma, Haibin AU - Ma H AD - Key Laboratory of Ningxia Stem Cell and Regenerative Medicine, Institute of Medical Sciences, General Hospital of Ningxia Medical University, Yinchuan, China. FAU - Niu, Zhanfeng AU - Niu Z AUID- ORCID: 0000-0003-3297-1429 AD - Department of Neurosurgery, General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China. LA - eng GR - 82160266/the National Natural Science Foundation of China/ GR - 2019AAC03224/the Natural Science Foundation of Ningxia Province/ GR - 2025AAC030802/the Natural Science Foundation of Ningxia Province/ PT - Journal Article DEP - 20261029 PL - United States TA - Ann Clin Transl Neurol JT - Annals of clinical and translational neurology JID - 101623278 SB - IM PMC - PMC13717412 OTO - NOTNLM OT - endocrine alterations OT - functional outcome OT - inflammation OT - thyroid hormones OT - traumatic brain injury COIS- Preprint Statement: An earlier version of this manuscript was posted on Research Square as a preprint entitled "Acute Multi-Axis Endocrine Alterations After Traumatic Brain Injury: Associations With Injury Severity and 6-Month Functional Outcome." The preprint is available at: https://doi.org/10.21203/rs.3.rs-9597854/v1. The authors declare no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 PMCR- 2026/10/29 CRDT- 2026/10/29 04:44 PHST- 2026/09/17 00:00 [revised] PHST- 2026/07/14 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:44 [entrez] PHST- 2026/10/29 00:00 [pmc-release] AID - ACN370570 [pii] AID - 10.1002/acn3.70570 [doi] PST - aheadofprint SO - Ann Clin Transl Neurol. 2026 Oct 29:10.1002/acn3.70570. doi: 10.1002/acn3.70570. PMID- 42952441 OWN - NLM STAT- Publisher LR - 20261029 IS - 1346-8138 (Electronic) IS - 0385-2407 (Linking) DP - 2026 Oct 29 TI - When JAK Selectivity Matters: Worsening Urticaria and Recurrent Allergic Rhinitis After Switching From Baricitinib to Ritlecitinib in Alopecia Areata. LID - 10.1111/1346-8138.70559 [doi] FAU - Ito, Koki AU - Ito K AD - Department of Dermatology, Shiga University of Medical Science, Otsu, Shiga, Japan. FAU - Ikuno, Yasuaki AU - Ikuno Y AUID- ORCID: 0009-0003-1498-3802 AD - Department of Dermatology, Shiga University of Medical Science, Otsu, Shiga, Japan. FAU - Yamada, Masahiro AU - Yamada M AD - Department of Dermatology, Shiga University of Medical Science, Otsu, Shiga, Japan. FAU - Yamaguchi, Akihiko AU - Yamaguchi A AD - Department of Dermatology, Shiga University of Medical Science, Otsu, Shiga, Japan. FAU - Takahashi, Toshifumi AU - Takahashi T AUID- ORCID: 0000-0001-6059-9092 AD - Department of Dermatology, Shiga University of Medical Science, Otsu, Shiga, Japan. FAU - Arakawa, Akiko AU - Arakawa A AUID- ORCID: 0000-0002-6679-3029 AD - Department of Dermatology, Shiga University of Medical Science, Otsu, Shiga, Japan. AD - Department of Dermatology, Ludwig-Maximilian-University, Munich, Germany. AD - Department of Dermatology, Leipzig University, Leipzig, Germany. FAU - Fujimoto, Noriki AU - Fujimoto N AUID- ORCID: 0000-0003-4051-0649 AD - Department of Dermatology, Shiga University of Medical Science, Otsu, Shiga, Japan. LA - eng PT - Letter DEP - 20261029 PL - England TA - J Dermatol JT - The Journal of dermatology JID - 7600545 SB - IM EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 04:32 PHST- 2026/10/19 00:00 [revised] PHST- 2026/08/28 00:00 [received] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:32 [entrez] AID - 10.1111/1346-8138.70559 [doi] PST - aheadofprint SO - J Dermatol. 2026 Oct 29. doi: 10.1111/1346-8138.70559. PMID- 42952438 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1557-9042 (Electronic) IS - 0897-7151 (Linking) VI - 43 IP - 23-24 DP - 2026 Nov TI - Dynamic Changes of Glu and Asp Concentrations in Blood and Spinal Fluid of Patients with Traumatic Spinal Cord Injury and Their Correlation with Prognosis. PG - 1998-2007 LID - 10.1177/08977151251412821 [doi] AB - As excitatory neurotransmitters, glutamic acid (Glu) and aspartic acid (Asp) may play a key role in the pathological process of traumatic spinal cord injury. The purpose of this study was to investigate the dynamic changes of Glu and Asp concentrations in blood and spinal fluid of patients with secondary spinal cord injury and their correlation with prognosis, so as to provide evidence for disease monitoring and treatment. In this study, 62 patients with secondary spinal cord injury admitted from January 2021 to December 2023 were selected as study subjects. Blood and spinal fluid samples were collected at 1, 3, 7, 14, and 28 days after injury, and the concentrations of Glu and Asp were determined by high-performance liquid chromatography. At the same time, the neurological functions of the patients at admission and 3 months after injury were evaluated using the American Spinal Cord Injury Association (ASIA) scoring criteria, and the patients were divided into a good prognosis group (ASIA score increased by >/=10 points) and a poor prognosis group (ASIA score increased by <10 points). Repeated measurement ANOVA was used to compare the differences of Glu and Asp concentrations in blood and spinal fluid of patients and the control group at different time points, and Spearman correlation analysis was used to explore the correlation between Glu and Asp concentrations and changes in ASIA score. Logistic regression analysis was performed to determine the risk factors affecting the prognosis of patients, and a receiver operating characteristics (ROC) curve was drawn to evaluate the diagnostic value of relevant indicators for prognosis. The concentrations of Glu and Asp in blood and spinal fluid of patients with poor prognosis were higher than those of patients with good prognosis at all time points (p < 0.05). The concentrations of Glu and Asp in blood and spinal fluid of patients with poor prognosis were higher than those of patients with good prognosis at all time points (p < 0.05). Spearman correlation analysis showed that Glu and Asp concentrations in blood and spinal fluid were significantly negatively correlated with ASIA score (r = -0.65, p < 0.01; r = -0.58, p < 0.01). Logistic regression analysis showed that Glu concentration (OR = 2.58, 95% CI: 1.56-4.29) and Asp concentration (OR = 2.25, 95% CI: 1.32-3.84) in spinal fluid 7 days after injury were independent risk factors for prognosis. ROC curve showed that the area under the curve of the combined detection of Glu and Asp in spinal fluid at 7 days after injury was significantly higher than that of single index (p < 0.01). The concentrations of Glu and Asp in the blood and spinal fluid of patients with acute traumatic spinal cord injury increase first and then decrease, and are closely related to the prognosis of patients. Early monitoring of these neurotransmitter concentrations after injury is helpful to evaluate the prognosis of patients and provide a reference for the formulation of personalized treatment plans. ROC curve analysis further clarified the diagnostic efficacy of relevant indicators in prognostic judgment. FAU - Zhang, Xinyu AU - Zhang X AD - Emergency Department (Xiangjiang Campus), Hebei Medical University Third Hospital, Shijiazhuang, China. FAU - Zhang, Xiaobo AU - Zhang X AD - Emergency Department (Xiangjiang Campus), Hebei Medical University Third Hospital, Shijiazhuang, China. FAU - Yang, Dong AU - Yang D AD - Emergency Department (Xiangjiang Campus), Hebei Medical University Third Hospital, Shijiazhuang, China. FAU - Huo, Xiulin AU - Huo X AD - Department of Anesthesiology, Hebei Medical University Third Hospital, Shijiazhuang, China. FAU - Liu, Li AU - Liu L AD - Emergency Department (Xiangjiang Campus), Hebei Medical University Third Hospital, Shijiazhuang, China. FAU - Jia, Liwei AU - Jia L AD - Emergency Department (Xiangjiang Campus), Hebei Medical University Third Hospital, Shijiazhuang, China. FAU - Deng, Zhuojun AU - Deng Z AD - Emergency Department (Xiangjiang Campus), Hebei Medical University Third Hospital, Shijiazhuang, China. LA - eng PT - Journal Article DEP - 20260113 PL - United States TA - J Neurotrauma JT - Journal of neurotrauma JID - 8811626 RN - 3KX376GY7L (Glutamic Acid) RN - 30KYC7MIAI (Aspartic Acid) RN - 0 (Biomarkers) SB - IM MH - Humans MH - *Spinal Cord Injuries/blood/cerebrospinal fluid/diagnosis/metabolism MH - *Glutamic Acid/blood/cerebrospinal fluid MH - Prognosis MH - *Aspartic Acid/blood/cerebrospinal fluid MH - Female MH - Male MH - Adult MH - Middle Aged MH - Biomarkers/blood/cerebrospinal fluid OTO - NOTNLM OT - aspartate OT - dynamic change OT - glutamic acid OT - prognosis OT - spinal cord traumatic injury COIS- Author Disclosure StatementThe authors declare that they have no competing interests. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 CRDT- 2026/10/29 04:23 PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:23 [entrez] AID - 10.1177/08977151251412821 [doi] PST - ppublish SO - J Neurotrauma. 2026 Nov;43(23-24):1998-2007. doi: 10.1177/08977151251412821. Epub 2026 Jan 13. PMID- 42952434 OWN - NLM STAT- Publisher LR - 20261029 IS - 1362-301X (Electronic) IS - 0269-9052 (Linking) DP - 2026 Oct 29 TI - Hospital mortality and discharge destination prediction after fall-related traumatic brain injury in older adults. PG - 1-9 LID - 10.1080/02699052.2026.2748378 [doi] AB - OBJECTIVE: Develop prediction models to support care for older adults with fall-related traumatic brain injury (TBI). SUMMARY OF BACKGROUND: Fall-related TBI is a leading cause of hospitalization and death among older adults. Prognostication remains challenging in this population because frailty and comorbidity burden greatly influence recovery trajectories. Tailored prognostic models to support individualized patient care remain limited. PATIENTS AND METHODS: Data from 179,287 older adults (>/=65 years) hospitalized with fall-related TBI in the NTDB (2017-2021) were analyzed. MARS models were developed to predict in-hospital mortality and discharge destination (home, rehabilitation, or care facility). Predictor selection was guided by clinicians' input, and model performance was evaluated using discrimination and calibration measures. RESULTS: The mortality model showed high discrimination (AUC 0.88, 95% CI 0.87-0.89; balanced accuracy 79%). The discharge model demonstrated moderate-to-good discrimination across discharge destinations (AUCs 0.77-0.85; balanced accuracies 70%-77%). Both models showed good calibration and were implemented as interactive web-based tools. CONCLUSION: This study developed two prognostic models that provide individualized outcome probabilities to support prognostic communication and care planning for older adults hospitalized with fall-related TBI. FAU - Osong, Biche AU - Osong B AD - Center for Pediatric Trauma Research and Center for Injury Research and Policy, The Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA. FAU - Armstrong, Megan AU - Armstrong M AD - Center for Pediatric Trauma Research and Center for Injury Research and Policy, The Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA. FAU - Williams, Hannah AU - Williams H AD - Center for Pediatric Trauma Research and Center for Injury Research and Policy, The Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA. FAU - Sribnick, Eric A AU - Sribnick EA AD - Center for Pediatric Trauma Research and Center for Injury Research and Policy, The Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA. AD - Division of Pediatric Neurosurgery, Nationwide Children's Hospital, Columbus, Ohio, USA. AD - Department of Pediatrics, The Ohio State University College of Medicine, Columbus, Ohio, USA. FAU - Groner, Jonathan I AU - Groner JI AD - Center for Pediatric Trauma Research and Center for Injury Research and Policy, The Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA. AD - Department of Pediatrics, The Ohio State University College of Medicine, Columbus, Ohio, USA. AD - Division of Pediatric Surgery, Nationwide Children's Hospital, Columbus, Ohio, USA. FAU - Xiang, Henry AU - Xiang H AD - Center for Pediatric Trauma Research and Center for Injury Research and Policy, The Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA. AD - Department of Pediatrics, The Ohio State University College of Medicine, Columbus, Ohio, USA. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Brain Inj JT - Brain injury JID - 8710358 SB - IM OTO - NOTNLM OT - Traumatic brain injury OT - discharge destination OT - falls OT - mortality OT - multivariate adaptive regression splines OT - older adults EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 04:22 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:22 [entrez] AID - 10.1080/02699052.2026.2748378 [doi] PST - aheadofprint SO - Brain Inj. 2026 Oct 29:1-9. doi: 10.1080/02699052.2026.2748378. PMID- 42952433 OWN - NLM STAT- Publisher LR - 20261029 IS - 1724-6059 (Electronic) IS - 1121-8428 (Linking) DP - 2026 Oct 29 TI - Ultrasound-guided core-needle biopsy for the diagnosis of calciphylaxis in a peritoneal dialysis patient. LID - aajag272 [pii] LID - 10.1093/joneph/aajag272 [doi] AB - Calciphylaxis is a life-threatening and rare disease, also known as calcific uremic arteriolopathy (CUA). Skin biopsy is the gold standard for diagnosis. However, it is an invasive procedure and might aggravate the ulcer. Therefore, additional precise diagnostic approaches are needed. In this case report, a patient with end-stage renal disease receiving peritoneal dialysis presented with skin ulceration and necrosis on the left leg. Considering the risk of aggravating the existing ulcer, we performed an ultrasound-guided core-needle biopsy. Histopathological examination confirmed calcification of small- and medium-sized vessels in the dermis and subcutaneous tissue, supporting the diagnosis of calciphylaxis. The patient subsequently received comprehensive treatment, including sodium thiosulfate therapy, wound care, correction of chronic kidney disease-mineral and bone disorder, and adjustment of dialysis therapy. With comprehensive and timely treatment, the patient's skin lesions gradually improved and showed progressive healing. This case suggests that ultrasound-guided core-needle biopsy may represent a feasible diagnostic option for calciphylaxis, providing histopathological confirmation. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Italian Society of Nephrology. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Gao, Xiaojun AU - Gao X AD - Department of Nephrology, The Third People's Hospital of Chengdu, Southwest Jiaotong University Affiliated Hospital, Chengdu 610014, Sichuan, China. FAU - Zhang, Huijian AU - Zhang H AD - Department of Nephrology, The Third People's Hospital of Chengdu, Southwest Jiaotong University Affiliated Hospital, Chengdu 610014, Sichuan, China. FAU - Shu, Ying AU - Shu Y AD - Department of Nephrology, The Third People's Hospital of Chengdu, Southwest Jiaotong University Affiliated Hospital, Chengdu 610014, Sichuan, China. FAU - Liang, Zhu AU - Liang Z AD - Department of Nephrology, The Third People's Hospital of Chengdu, Southwest Jiaotong University Affiliated Hospital, Chengdu 610014, Sichuan, China. FAU - Tong, Yuna AU - Tong Y AD - Department of Nephrology, The Third People's Hospital of Chengdu, Southwest Jiaotong University Affiliated Hospital, Chengdu 610014, Sichuan, China. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - J Nephrol JT - Journal of nephrology JID - 9012268 SB - IM OTO - NOTNLM OT - Calciphylaxis OT - Core-needle biopsy OT - Hyperparathyroidism OT - Sodium thiosulfate EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 04:13 PHST- 2026/02/13 00:00 [received] PHST- 2026/07/01 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:13 [entrez] AID - 9004277 [pii] AID - 10.1093/joneph/aajag272 [doi] PST - aheadofprint SO - J Nephrol. 2026 Oct 29:aajag272. doi: 10.1093/joneph/aajag272. PMID- 42952428 OWN - NLM STAT- Publisher LR - 20261029 IS - 1532-298X (Electronic) IS - 1040-4651 (Linking) DP - 2026 Oct 29 TI - Allelic tuning of PtoRLK609 kinase activity shapes adaptive xylem remodeling in Populus tomentosa. LID - koag328 [pii] LID - 10.1093/plcell/koag328 [doi] AB - Climate change poses unprecedented challenges to perennial trees. However, the molecular mechanisms by which trees achieve long-term adaptive xylem remodeling remain poorly understood. Here, using transcriptomics and population genomics in 302 Populus tomentosa accessions sampled from three climatically distinct subpopulations, we identified receptor-like kinases (RLKs) associated with climate-related variation in xylem lignification. Among 49 candidates, the wall-associated kinase PtoRLK609 showed among the strongest signatures of population differentiation, a pattern consistent with climate-associated selection. Two major PtoRLK609 haplotypes, defined by four linked missense variants, showed marked geographic differentiation, with PtoRLK609Hap2 reaching fixation or near-fixation in northern populations that experience drier and cooler climates, and was associated with elevated lignin content. PtoRLK609 phosphorylates the transcription factor PtoERF105 at Ser171, which promotes its nuclear accumulation and enhances activation of the lignin biosynthesis genes PtoLAC4 and PtoCCoAOMT1, as well as the vessel development regulator PtoVND7. The PtoRLK609Hap2 protein variant showed higher kinase activity and more efficient phosphorylation of PtoERF105 than PtoRLK609Hap1, linking natural protein variation to differential transcriptional output. Consistent with this mechanism, gain- and loss-of-function analyses showed that PtoRLK609 promotes secondary cell wall lignification, elevates the syringyl-to-guaiacyl (S/G) ratio, increases vessel density, and is associated with better maintenance of stem water status under controlled water deficit. These findings characterize a PtoRLK609-PtoERF105 phosphorylation module associated with xylem remodeling and identify an RLK locus that is a strong candidate for contributing to climate-associated divergence in perennial trees. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of American Society of Plant Biologists. FAU - Qi, Weina AU - Qi W AD - State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. AD - National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. FAU - Wen, Zheng AU - Wen Z AD - State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. FAU - Huang, Weixiong AU - Huang W AUID- ORCID: 0000-0002-4020-8629 AD - State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. AD - National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. FAU - Ren, Tailin AU - Ren T AD - State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. AD - National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. FAU - Xiao, Liang AU - Xiao L AUID- ORCID: 0000-0001-6683-8955 AD - State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. AD - National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. FAU - Fang, Yuanyuan AU - Fang Y AUID- ORCID: 0009-0007-2059-7069 AD - School of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China. FAU - Li, Yunzhou AU - Li Y AUID- ORCID: 0009-0003-9220-7593 AD - State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. AD - National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. FAU - Yuan, Xin AU - Yuan X AD - State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. AD - National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. FAU - Cheng, Wenzhuang AU - Cheng W AD - State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. AD - National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. FAU - He, Wei AU - He W AD - State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. AD - National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. FAU - Quan, Mingyang AU - Quan M AUID- ORCID: 0000-0001-5301-1801 AD - State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. AD - National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. FAU - Du, Qingzhang AU - Du Q AUID- ORCID: 0000-0003-1410-2609 AD - State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. AD - National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. FAU - Ingvarsson, Par K AU - Ingvarsson PK AUID- ORCID: 0000-0001-9225-7521 AD - Department of Plant Biology, Swedish University of Agricultural Sciences, 750 07 Uppsala, Sweden. FAU - El-Kassaby, Yousry A AU - El-Kassaby YA AUID- ORCID: 0000-0002-4887-8977 AD - Department of Forest and Conservation Sciences, Faculty of Forestry, The University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada. FAU - Zhang, Deqiang AU - Zhang D AUID- ORCID: 0000-0002-8849-2366 AD - State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. AD - National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Plant Cell JT - The Plant cell JID - 9208688 SB - IM EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 04:13 PHST- 2025/11/02 00:00 [received] PHST- 2026/04/28 00:00 [revised] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:13 [entrez] AID - 9004285 [pii] AID - 10.1093/plcell/koag328 [doi] PST - aheadofprint SO - Plant Cell. 2026 Oct 29:koag328. doi: 10.1093/plcell/koag328. PMID- 42952426 OWN - NLM STAT- Publisher LR - 20261029 IS - 1095-8290 (Electronic) IS - 0305-7364 (Linking) DP - 2026 Oct 29 TI - Life in the fast lane: early-successional species show four different strategies. LID - mcag344 [pii] LID - 10.1093/aob/mcag344 [doi] AB - BACKGROUND AND AIMS: Global land-use change and climate change are transforming tropical forests into regrowing secondary forests, where early-successional species play a crucial role by colonizing abandoned lands and paving the way for later-successional species. While often considered a homogeneous group with uniformly fast strategies, this study evaluates the diversity of early-successional strategies from Ghanaian tropical dry and wet forests. METHODS: We selected 30 early-successional species from Ghanaian dry and wet forests differing in leaf habit (deciduous vs. evergreen), and measured wood density and 12 morphological and physiological leaf traits related to drought adaptation and resource acquisition. Traits were compared between forest types and leaf habits using two-way ANOVA, and multivariate analyses identified trait associations and trade-offs. KEY RESULTS: Dry forest species were more drought-tolerant and had slower gas exchange rates than wet forest species, reflecting reduced water use under limited water availability. Multivariate trait analysis showed that early-successional species span a two-dimensional trait space with two main trade-offs: (1) drought tolerance (i.e. high absolute leaf turgor loss point and wood density) versus gas exchange, and (2) investment in leaf structural carbon versus nutrients. The two axes defined four strategies: species that (1) tolerate drought and have tough leaves ("tolerant-and-tough"), (2) tolerate drought and have nutrient-rich leaves ("tolerant-and-rich"), (3) grow rapidly and have tough leaves ("fast-and-tough"), and (4) grow rapidly and have nutrient-rich leaves ("fast-and-rich"). CONCLUSIONS: Early-successional species followed four diverse strategies rather than converging on a single, fast-growing, acquisitive strategy. Their strategies also extended beyond the commonly described fast-slow gradient, with unexpected trait combinations reflecting the contrasting demands of rapid carbon gain and water conservation during early succession. Such variation may steer successional trajectories because different environmental conditions select for different early-successional strategies. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Kok, Jazz J M AU - Kok JJM AUID- ORCID: 0000-0002-1549-3376 AD - Wageningen University, Forest Ecology and Forest Management Group, PO Box 47, Wageningen, 6700 AA, The Netherlands. FAU - Bongers, Frans AU - Bongers F AD - Wageningen University, Forest Ecology and Forest Management Group, PO Box 47, Wageningen, 6700 AA, The Netherlands. FAU - Amissah, Lucy AU - Amissah L AD - CSIR Forestry Research Institute of Ghana, UPO Box 63, Kumasi, Ghana. AD - CSIR College of Science and Technology, P. O. Box M 32, Accra, Ghana. FAU - Baah, Alexander AU - Baah A AD - Kwame Nkrumah University of Science and Technology, GPS AK-448-4944, Kumasi, Ghana. FAU - Matsuo, Tomonari AU - Matsuo T AD - Wageningen University, Forest Ecology and Forest Management Group, PO Box 47, Wageningen, 6700 AA, The Netherlands. FAU - Poorter, Lourens AU - Poorter L AUID- ORCID: 0000-0003-1391-4875 AD - Wageningen University, Forest Ecology and Forest Management Group, PO Box 47, Wageningen, 6700 AA, The Netherlands. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Ann Bot JT - Annals of botany JID - 0372347 SB - IM OTO - NOTNLM OT - Ghana OT - drought tolerance OT - early-successional tree species OT - functional traits OT - gas exchange OT - species strategies OT - succession OT - tropical forest EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 04:13 PHST- 2025/06/02 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:13 [entrez] AID - 9004269 [pii] AID - 10.1093/aob/mcag344 [doi] PST - aheadofprint SO - Ann Bot. 2026 Oct 29:mcag344. doi: 10.1093/aob/mcag344. PMID- 42952420 OWN - NLM STAT- Publisher LR - 20261029 IS - 1460-2237 (Electronic) IS - 0268-1080 (Linking) DP - 2026 Oct 29 TI - Policy coherence for resilient health systems: Do Sierra Leone's health sector strategies active in the 2025 to 2026 planning window pull in the same direction? LID - czag122 [pii] LID - 10.1093/heapol/czag122 [doi] AB - Sierra Leone is entering a dense period of health sector strategising, with multiple frameworks active across system strengthening, partner coordination, digital transformation, health security, and water, sanitation and hygiene (WASH). Their coexistence raises a critical question: do they reinforce a coherent agenda for resilient, equitable universal health coverage (UHC) or generate fragmentation and implementation burden? We conducted a qualitative health policy analysis combining documentary review of five national strategies, the National Health Sector Strategic Plan (NHSSP) 2026 to 2030, the National Health Compact 2025, the Digital Health Roadmap 2024 to 2026, the National Action Plan for Health Security 2025 to 2029, and the WASH Sector Strategic Plan 2026 to 2035, together with selected Ministry of Health frameworks, and sixteen semi structured key informant interviews. Data were analysed using the Framework Method, guided conceptually by a policy coherence lens spanning strategic priorities, governance, monitoring, financing, and district level implications. The strategies converged around primary health care, equity, service readiness, surveillance, workforce strengthening, WASH, and improved data systems. Coherence was strongest at the level of aspiration and weakest in operational architecture, with three recurrent fault lines: parallel governance structures, fragmented monitoring frameworks, and limited translation of cross cutting ambition into integrated district implementation. Documentary analysis showed misaligned planning horizons, including a Digital Health Roadmap that expires within the first year of the umbrella NHSSP, and indicator sets that define shared priorities differently across documents. Sierra Leone's policy portfolio is directionally aligned but operationally fragmented. Strengthening coherence will require a clearer strategy hierarchy, a unified results framework, rationalised coordination, embedded digital governance, stronger health, WASH and health security integration, and financing that supports cross programme implementation. Findings derive from a purposive sample and are analytical rather than statistically representative. CI - (c) The Author(s) 2026. Published by Oxford University Press in association with The London School of Hygiene and Tropical Medicine. FAU - Osborne, Augustus AU - Osborne A AUID- ORCID: 0000-0002-0226-841X AD - Institute for Development, Western area, Freetown, Sierra Leone. FAU - Mustapha, Mariama AU - Mustapha M AD - College of Medicine and Allied Health Sciences, University of Sierra Leone, Western area, Freetown, Sierra Leone. FAU - Vandy, Ahmed AU - Vandy A AUID- ORCID: 0009-0008-2603-9989 AD - College of Medicine and Allied Health Sciences, University of Sierra Leone, Western area, Freetown, Sierra Leone. FAU - Njai, Abdul Jibril AU - Njai AJ AD - Ministry of Health, Western area, Freetown, Sierra Leone. FAU - Moses, Francis AU - Moses F AD - Ministry of Health, Western area, Freetown, Sierra Leone. FAU - Kabba, Mustapha AU - Kabba M AD - Ministry of Health, Western area, Freetown, Sierra Leone. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Health Policy Plan JT - Health policy and planning JID - 8610614 SB - IM OTO - NOTNLM OT - Sierra Leone OT - health policy analysis OT - health systems resilience OT - policy coherence OT - strategic planning OT - universal health coverage EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 04:13 PHST- 2026/06/14 00:00 [received] PHST- 2026/08/25 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:13 [entrez] AID - 9004279 [pii] AID - 10.1093/heapol/czag122 [doi] PST - aheadofprint SO - Health Policy Plan. 2026 Oct 29:czag122. doi: 10.1093/heapol/czag122. PMID- 42952408 OWN - NLM STAT- Publisher LR - 20261029 IS - 1521-3773 (Electronic) IS - 1433-7851 (Linking) DP - 2026 Oct 29 TI - pH-Driven Conformational Switching of Binaphthyl-Pyrene Imidazolium Macrocycles Enables Tunable Chiroptical Recognition of Dinucleotide Sequences in Water. LID - e5363221 [pii] LID - 10.1002/anie.5363221 [doi] AB - Pyrene-based excimer chiral luminescent systems represent promising platforms for constructing multifunctional chiroptical materials, as excimer formation can simultaneously generate fluorescence (FL), circular dichroism (CD), and circularly polarized luminescence (CPL) signals. In this work, we report two enantiomeric pairs of binaphthyl-pyrene imidazolium macrocycles (RR/SS-1.4Cl- and RR/SS-2.4Cl-) that integrate confined pyrene excimers, water-soluble recognition sites, and dynamic chiral cavity into a single macrocyclic scaffold. All macrocycles exhibit intramolecular pyrene excimer emission, characteristic CD, and strong CPL. The phenolic hydroxyl groups endow RR-1.4Cl- with reversible pH-driven conformational switching between closed-cavity and open-cavity structures, enabling reversible regulation of FL, CD, and CPL signals. Host-guest studies reveal two distinct dinucleotide recognition modes: surface-associated guests preserve the pyrene-stacking arrangement, whereas cavity-encapsulated guests disrupt the confined pyrene stacking and induce pronounced multiple optical responses. Upon deprotonation, the open-cavity conformation of RR-1.4Cl- selectively enhances interactions with cavity-preferred dinucleotides, resulting in enhanced sequence-selective recognition. By integrating CD and FL responses as multidimensional optical fingerprints, a single macrocyclic sensor enables accurate discrimination of all 16 dinucleotide sequences through heatmap analysis and principal component analysis (PCA). This work demonstrates a general strategy for constructing adaptive chiral macrocycles that translate subtle biomolecular sequence variations into amplified optical signatures. CI - (c) 2026 Wiley-VCH GmbH. FAU - He, Tianci AU - He T AD - Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, P. R. China. FAU - Huo, Mengxin AU - Huo M AD - Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, P. R. China. FAU - He, Dingyu AU - He D AD - Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, P. R. China. FAU - Xiang, Song AU - Xiang S AD - Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, P. R. China. FAU - Yang, Ting AU - Yang T AD - Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, P. R. China. FAU - Huo, Haoyang AU - Huo H AD - Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, P. R. China. FAU - Su, Feng AU - Su F AUID- ORCID: 0009-0003-7734-9331 AD - Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, P. R. China. FAU - Yu, Yang AU - Yu Y AD - Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, P. R. China. FAU - Cheng, Lin AU - Cheng L AD - Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, P. R. China. FAU - Cao, Liping AU - Cao L AUID- ORCID: 0000-0002-1747-6107 AD - Key Laboratory of Synthetic and Natural Functional Molecule of the Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, P. R. China. LA - eng GR - 22571250/National Natural Science Foundation of China/ GR - 22371229/National Natural Science Foundation of China/ GR - the Sanqin Talent Special Support Program/ GR - 2026RS-CXTD-28/the Innovation Capability Support Program of Shaanxi/ GR - sklscbs202529/the Open Project of State Key Laboratory of Synergistic Chem-Bio Synthesis/ PT - Journal Article DEP - 20261029 PL - Germany TA - Angew Chem Int Ed Engl JT - Angewandte Chemie (International ed. in English) JID - 0370543 SB - IM OTO - NOTNLM OT - chiroptical recognition OT - conformational switching OT - dinucleotide OT - macrocycles OT - pyrene excimer EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 04:03 PHST- 2026/10/12 00:00 [revised] PHST- 2026/09/16 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:03 [entrez] AID - e5363221 [pii] AID - 10.1002/anie.5363221 [doi] PST - aheadofprint SO - Angew Chem Int Ed Engl. 2026 Oct 29:e5363221. doi: 10.1002/anie.5363221. PMID- 42952404 OWN - NLM STAT- Publisher LR - 20261029 IS - 1521-3773 (Electronic) IS - 1433-7851 (Linking) DP - 2026 Oct 29 TI - Ribozyme Redesign From an Existing RNA Scaffold Enables Multifunctional Hydrolysis for Molecular Release. LID - e4905134 [pii] LID - 10.1002/anie.4905134 [doi] AB - The discovery of novel ribozymes is crucial for both advancing RNA technologies and illuminating potential RNA world scenarios. Currently, catalytic evolution of ribozymes primarily relies on the slow, random process of natural or artificial selection. In this study, we show that a distinct catalytic feature can be deliberately imposed onto a preexisting ribozyme scaffold through ribozyme redesign with a minimal alteration rather than molecular evolution. We demonstrate a single-nucleotide mutation repurposes an alkyl-transfer ribozyme into a multifunctional hydrolysis ribozyme, while preserving its overall catalytic scaffold. This hydrolase ribozyme operates analogously to a canonical protein enzyme, transforming various ester and ether substrates into the release of carboxylic acids, alcohols, and amines. Structural and kinetic analyses uncover a water-mediated mechanism for ester/ether bond cleavage. Application of this hydrolase ribozyme in living cells enables controlled activation and release of diverse payloads on demand, such as fluorescent and drug molecules, for cell-specific discrimination. This study highlights the previously underappreciated multifunctionalities within RNA catalytic architectures, broadening the scope of developing versatile ribozymes for diversified applications. CI - (c) 2026 Wiley-VCH GmbH. FAU - Yan, Jun AU - Yan J AD - MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou, China. FAU - He, Yuan-Lin AU - He YL AD - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangdong-Hong Kong Joint Laboratory for RNA Medicine, Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China. FAU - Zhang, Chen-Xi AU - Zhang CX AD - MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou, China. FAU - Ye, Sen-Lin AU - Ye SL AD - MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou, China. FAU - Li, Xiao-Hui AU - Li XH AD - MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou, China. FAU - Wu, Feng-Tai AU - Wu FT AD - MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou, China. FAU - Wang, Liang-Liang AU - Wang LL AD - MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou, China. FAU - Huang, Lin AU - Huang L AUID- ORCID: 0000-0002-2121-365X AD - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Guangdong-Hong Kong Joint Laboratory for RNA Medicine, Medical Research Center, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China. FAU - Xu, Liang AU - Xu L AUID- ORCID: 0000-0003-1239-4476 AD - MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-Sen University, Guangzhou, China. LA - eng GR - 22477144/National Natural Science Foundation of China/ GR - 22222706/National Natural Science Foundation of China/ GR - 22507053/National Natural Science Foundation of China/ GR - 32171191/National Natural Science Foundation of China/ GR - 2020YFA0211200/National Key R&D Program of China/ GR - 2024B1515040028/GBRCE for Functional Molecular Engineering, Guangdong Basic and Applied Basic Research Foundation/ PT - Journal Article DEP - 20261029 PL - Germany TA - Angew Chem Int Ed Engl JT - Angewandte Chemie (International ed. in English) JID - 0370543 SB - IM OTO - NOTNLM OT - RNA catalysis OT - RNA technology OT - molecular release OT - multifunctional hydrolysis OT - ribozyme engineering EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 04:03 PHST- 2026/10/21 00:00 [revised] PHST- 2026/07/17 00:00 [received] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 04:03 [entrez] AID - e4905134 [pii] AID - 10.1002/anie.4905134 [doi] PST - aheadofprint SO - Angew Chem Int Ed Engl. 2026 Oct 29:e4905134. doi: 10.1002/anie.4905134. PMID- 42952379 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1537-2537 (Electronic) IS - 0047-2425 (Print) IS - 0047-2425 (Linking) VI - 55 IP - 6 DP - 2026 Nov-Dec TI - Association of runoff risk with private well contamination in a fractured dolostone aquifer. LID - e70296 [pii] LID - 10.1002/jeq2.70296 [doi] AB - In the United States, private well owners are responsible for monitoring and maintaining their drinking water but have little control over neighboring contamination sources and limited information about daily water quality. Potential for rapid contamination in karst settings exacerbates the challenge. The Runoff Risk Advisory Forecast (RRAF) is a real-time online tool available in five US states that helps nutrient applicators avoid times and locations of high runoff risk. We wanted to determine if RRAF could be repurposed in areas susceptible to groundwater contamination to inform well owners of conditions when contamination is likely. Private wells were repeatedly sampled on seven dates (32-36 wells per date) and analyzed for nitrate-nitrogen, total coliforms, and bovine manure markers. Three-day windows of RRAF risk prior to sampling were selected post hoc, ranging from 1 to 3 days prior for manure markers to 10-12 days prior for nitrate. Contaminants were associated with RRAF values of >/=75% within these windows based on observed weather. A hydrologic association between RRAF risk and groundwater depth was also apparent. Overall, results suggest the RRAF model anticipates conditions of elevated contamination risk for susceptible wells. For nitrate, between-well variation and delayed peak concentration suggested that well location and construction factors may be more informative than runoff events. Microbial contamination was rapid and distributed among wells, with one or more detections of coliforms in 31% of wells and bovine markers in 61% of wells. Thus, a warning system based on the RRAF model could help identify conditions of acute pathogen exposure. CI - (c) 2026 The Author(s). Journal of Environmental Quality published by Wiley Periodicals LLC on behalf of American Society of Agronomy, Crop Science Society of America, and Soil Science Society of America. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA. FAU - Heffron, Joe AU - Heffron J AUID- ORCID: 0000-0002-5110-9271 AD - USDA-ARS Environmentally Integrated Dairy Management Research Unit, Marshfield, Wisconsin, USA. AD - U. S. Geological Survey and USDA-ARS Laboratory for Infectious Disease and the Environment, Marshfield, Wisconsin, USA. FAU - Stokdyk, Joel P AU - Stokdyk JP AUID- ORCID: 0000-0003-2887-6277 AD - U. S. Geological Survey and USDA-ARS Laboratory for Infectious Disease and the Environment, Marshfield, Wisconsin, USA. AD - U.S. Geological Survey, Upper Midwest Water Science Center, Marshfield, Wisconsin, USA. FAU - Burch, Tucker R AU - Burch TR AUID- ORCID: 0000-0001-5756-514X AD - USDA-ARS Environmentally Integrated Dairy Management Research Unit, Marshfield, Wisconsin, USA. AD - U. S. Geological Survey and USDA-ARS Laboratory for Infectious Disease and the Environment, Marshfield, Wisconsin, USA. FAU - Cook, Rachel M AU - Cook RM AUID- ORCID: 0009-0007-3505-7946 AD - USDA-ARS Environmentally Integrated Dairy Management Research Unit, Marshfield, Wisconsin, USA. AD - U. S. Geological Survey and USDA-ARS Laboratory for Infectious Disease and the Environment, Marshfield, Wisconsin, USA. FAU - Firnstahl, Aaron D AU - Firnstahl AD AUID- ORCID: 0000-0003-2686-7596 AD - U. S. Geological Survey and USDA-ARS Laboratory for Infectious Disease and the Environment, Marshfield, Wisconsin, USA. AD - U.S. Geological Survey, Upper Midwest Water Science Center, Marshfield, Wisconsin, USA. FAU - Young, Eric O AU - Young EO AUID- ORCID: 0000-0001-9319-6521 AD - USDA-ARS Environmentally Integrated Dairy Management Research Unit, Marshfield, Wisconsin, USA. FAU - Sherman, Jessica F AU - Sherman JF AUID- ORCID: 0000-0003-0595-9707 AD - USDA-ARS Environmentally Integrated Dairy Management Research Unit, Marshfield, Wisconsin, USA. FAU - Goering, Dustin AU - Goering D AUID- ORCID: 0009-0001-0737-4318 AD - U.S. National Weather Service, North Central River Forecast Center, Chanhassen, Minnesota, USA. FAU - Kieke, Burney A Jr AU - Kieke BA Jr AUID- ORCID: 0000-0002-7430-0570 AD - Center for Clinical Epidemiology and Population Health, Marshfield Clinic Research Institute, Marshfield, Wisconsin, USA. FAU - Borchardt, Mark A AU - Borchardt MA AUID- ORCID: 0000-0002-6471-2627 AD - USDA-ARS Environmentally Integrated Dairy Management Research Unit, Marshfield, Wisconsin, USA. AD - U. S. Geological Survey and USDA-ARS Laboratory for Infectious Disease and the Environment, Marshfield, Wisconsin, USA. LA - eng GR - USDA/ GR - 5090-12630006-000D/Agricultural Research Service/ PT - Journal Article PL - United States TA - J Environ Qual JT - Journal of environmental quality JID - 0330666 RN - 0 (Water Pollutants, Chemical) RN - 0 (Nitrates) RN - 0 (Manure) SB - IM MH - *Groundwater/chemistry MH - *Environmental Monitoring MH - *Water Pollutants, Chemical/analysis MH - Animals MH - Nitrates/analysis MH - *Water Pollution MH - Cattle MH - Manure/analysis MH - Water Wells MH - Water Supply PMC - PMC13717291 OAB - In the United States, private wells are not regulated at the federal level. Individual well owners must monitor water quality and mitigate hazards on their own initiative, but they have little control over most factors affecting their well's water quality and few tools to assess health risks. This research aimed to evaluate an online tool for predicting surface water runoff as an early warning system for private well contamination in a region of intensive agriculture and rapid infiltration to groundwater. Runoff risk corresponded to changes in groundwater levels, indicating relevance for well water. High runoff risk was associated with microbial and nutrient contamination in 36 private wells that draw water from a vulnerable karst aquifer. This study demonstrates the feasibility of repurposing an existing tool in five US states to alert well owners of impending, acute contamination events. OABL- eng COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/29 CRDT- 2026/10/29 03:53 PHST- 2026/03/03 00:00 [received] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:53 [entrez] PHST- 2026/10/29 00:00 [pmc-release] AID - e70296 [pii] AID - JEQ270296 [pii] AID - 10.1002/jeq2.70296 [doi] PST - ppublish SO - J Environ Qual. 2026 Nov-Dec;55(6):e70296. doi: 10.1002/jeq2.70296. PMID- 42952377 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2045-7634 (Electronic) IS - 2045-7634 (Linking) VI - 15 IP - 11 DP - 2026 Nov TI - Genomic Alterations in Pulmonary Carcinoid Tumors: A Targeted Analysis of Gene Rearrangements and Oncogenic Mutations. LID - e72441 [pii] LID - 10.1002/cam4.72441 [doi] AB - INTRODUCTION: Pulmonary carcinoids (PCs) are rare, well-differentiated, neuroendocrine lung tumors with limited treatment options for advanced disease, underscoring the need for targeted therapies. PCs typically exhibit a low mutational burden. Although MEN1 and chromatin-modifier mutations are known contributors, potentially actionable alterations remain poorly characterized. To better define therapeutic opportunities, we evaluated oncogenic fusions and variants across 17 cancer-associated genes in a large retrospective PC cohort. METHODS: Formalin-fixed, paraffin-embedded primary tumors from Helsinki University Hospital (n = 119, collected between 1990 and 2020) were subjected to targeted RNA-based fusion and variant analysis using the Archer FusionPlex Lung v2 assay. RESULTS: The most frequent recurrent genetic rearrangements involved ALK (n = 5), BRAF (n = 3), FGFR2 (n = 3), and NTRK3 (n = 2), each featuring diverse fusion partners. Variant analysis identified BRAF, EGFR, FGFR1, and FGFR3 as the most frequently mutated genes, although only 2.2% (n = 4) of all detected variants were classified as oncogenic. Notably, 66.7% of all oncogenic or likely oncogenic variants occurred in BRAF and EGFR. Oncogenic variants were identified in ALK, BRAF, EGFR, and ERBB2. Although all FGFR1-3 variants were of uncertain significance, their frequency (34.1% of all detected variants), together with the three FGFR2 fusions, suggested that FGFR alterations may be biologically relevant in PCs. CONCLUSION: Despite the typically muted genomic landscape of PCs, our study revealed a small yet therapeutically relevant subset of alterations. The findings further characterized the molecular profile of PCs and identified potentially actionable molecular subgroups. While these alterations were not associated with disease-specific survival or metastatic status, they support consideration of molecular profiling in selected patients with advanced disease. CI - (c) 2026 The Author(s). Cancer Medicine published by John Wiley & Sons Ltd. FAU - Niinimaki, Jenni AU - Niinimaki J AUID- ORCID: 0009-0009-8342-0230 AD - Department of Pathology, HUS Diagnostic Center, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. FAU - Kytola, Soili AU - Kytola S AUID- ORCID: 0000-0002-4477-0942 AD - Department of Genetics, HUS Diagnostic Center, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. FAU - Ristimaki, Ari AU - Ristimaki A AUID- ORCID: 0000-0002-5373-9234 AD - Department of Pathology, HUS Diagnostic Center, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. AD - Applied Tumor Genomics Research Program, Research Programs Unit, University of Helsinki, Helsinki, Finland. FAU - Arola, Johanna AU - Arola J AUID- ORCID: 0000-0003-3865-5507 AD - Department of Pathology, HUS Diagnostic Center, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. FAU - Vesterinen, Tiina AU - Vesterinen T AUID- ORCID: 0000-0003-2997-0337 AD - Department of Pathology, HUS Diagnostic Center, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. LA - eng GR - Cancer Foundation Finland/ GR - TYH2021204/Helsinki University Hospital Research Fund/ PT - Journal Article PL - United States TA - Cancer Med JT - Cancer medicine JID - 101595310 RN - EC 2.7.11.1 (Proto-Oncogene Proteins B-raf) RN - EC 2.7.10.1 (Anaplastic Lymphoma Kinase) RN - EC 2.7.10.1 (ALK protein, human) RN - 0 (Biomarkers, Tumor) RN - EC 2.7.11.1 (BRAF protein, human) SB - IM MH - Humans MH - *Lung Neoplasms/genetics/pathology MH - *Carcinoid Tumor/genetics/pathology MH - *Gene Rearrangement MH - *Mutation MH - Female MH - Male MH - Retrospective Studies MH - Middle Aged MH - Proto-Oncogene Proteins B-raf/genetics MH - Aged MH - Adult MH - Genomics MH - Anaplastic Lymphoma Kinase/genetics MH - *Biomarkers, Tumor/genetics PMC - PMC13717302 OTO - NOTNLM OT - gene rearrangements OT - molecular profiling OT - neuroendocrine tumor OT - pulmonary carcinoid OT - variants COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/29 CRDT- 2026/10/29 03:53 PHST- 2026/09/14 00:00 [revised] PHST- 2026/03/30 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:53 [entrez] PHST- 2026/10/29 00:00 [pmc-release] AID - e72441 [pii] AID - CAM472441 [pii] AID - 10.1002/cam4.72441 [doi] PST - ppublish SO - Cancer Med. 2026 Nov;15(11):e72441. doi: 10.1002/cam4.72441. PMID- 42952375 OWN - NLM STAT- Publisher LR - 20261029 IS - 1521-4095 (Electronic) IS - 0935-9648 (Linking) DP - 2026 Oct 29 TI - Biointerface-Customized Nanomodulators Induce Mitochondria-Specific Dysfunction to Reprogram Tumor Immunity. LID - e75542 [pii] LID - 10.1002/adma.75542 [doi] AB - Tumor mitochondria regulate cancer progression and immune evasion. However, current therapeutic strategies lack the subcellular precision required to selectively induce immune-beneficial mitochondrial dysfunction without causing systemic toxicity. Here, we report a biointerface-customized nanomodulator (C/C-HP@M2M-TPP), developed through a biointerface-engineered strategy (BES), that integrates sequential targeting and controlled activation to induce mitochondria-specific dysfunction and reprogram tumor immunity. Through a customized biointerface coating, the nanomodulators achieve preferential tumor accumulation and mitochondria-directed subcellular localization. Co-delivered calcium ion donors and efflux inhibitors cooperatively induce mitochondrial ion interference and dysfunction, which is further amplified by photothermal activation. This localized mitochondrial interference promotes immunogenic cell death and robust antitumor immune activation, resulting in potent tumor regression in mice. Integrated single-cell transcriptomic and proteomic analyses further identify mitochondrial disruption as a key mechanistic driver of tumor immune reprogramming. This work establishes biointerface-customized nanomodulation as a subcellular immunomodulatory therapeutic strategy in vivo. CI - (c) 2026 Wiley-VCH GmbH. FAU - Zhen, Xueyan AU - Zhen X AUID- ORCID: 0009-0006-0611-529X AD - School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China. FAU - Chen, Yuxin AU - Chen Y AD - School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China. FAU - Li, Linhao AU - Li L AD - School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China. FAU - Zhang, Tingting AU - Zhang T AD - School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China. FAU - Li, Min AU - Li M AD - School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China. FAU - Wang, Runting AU - Wang R AD - School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China. FAU - Sun, Yihan AU - Sun Y AD - School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China. FAU - Li, Jiaqi AU - Li J AD - School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China. FAU - Ma, Shurui AU - Ma S AD - School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China. FAU - Zhou, Renyue AU - Zhou R AD - School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China. FAU - Wang, Sicen AU - Wang S AUID- ORCID: 0000-0002-2757-3277 AD - School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China. AD - Shaanxi Engineering Research Center of Cardiovascular Drugs Screening & Analysis, Xi'an, China. FAU - Xie, Xiaoyu AU - Xie X AUID- ORCID: 0000-0003-3936-3565 AD - School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, China. AD - Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education, Xi'an, China. LA - eng GR - 82273891/National Natural Science Foundation of China/ GR - 82373832/National Natural Science Foundation of China/ GR - U24A20796/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261029 PL - Germany TA - Adv Mater JT - Advanced materials (Deerfield Beach, Fla.) JID - 9885358 SB - IM OTO - NOTNLM OT - cancer research OT - cell biology OT - engineering OT - immune system OT - immunogenic cell death OT - in vivo OT - mitochondrion OT - programmed cell death OT - subcellular localization EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 03:53 PHST- 2026/09/30 00:00 [revised] PHST- 2026/02/02 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:53 [entrez] AID - e75542 [pii] AID - 10.1002/adma.75542 [doi] PST - aheadofprint SO - Adv Mater. 2026 Oct 29:e75542. doi: 10.1002/adma.75542. PMID- 42952372 OWN - NLM STAT- Publisher LR - 20261029 IS - 1521-3773 (Electronic) IS - 1433-7851 (Linking) DP - 2026 Oct 29 TI - Synthesis of a Stable Hydrophilic trans-Cyclooctene Amino Acid Unlocks Fast Bioorthogonal Click Chemistry for the Generation of Antibody-Drug Conjugates by Genetic Code Expansion. LID - e5717042 [pii] LID - 10.1002/anie.5717042 [doi] AB - The strain-promoted inverse electron demand Diels-Alder cycloaddition (SPIEDAC), usually employing trans-cyclooctenes (TCOs) and tetrazines, represents one of the fastest bioorthogonal click reactions currently available within the bioorthogonal "toolbox". TCOs can be conveniently introduced into proteins via genetic code expansion (GCE) technology, enabling a selectable site-specific incorporation of such noncanonical amino acids (ncAAs) at predefined positions. Their application under physiological conditions, however, is limited due their inherent instability toward alkene isomerization to the unreactive cis-isomer. This has limited the use of TCO-containing ncAAs for quantitative labeling applications, for example, of therapeutical bioconjugates. In this study, we report on the synthesis and application of a stable hydrophilic TCO-ncAA for efficient production of a highly efficacious antibody-drug conjugate (ADC) via GCE and subsequent SPIEDAC conjugation. CI - (c) 2026 The Author(s). Angewandte Chemie International Edition published by Wiley-VCH GmbH. FAU - Genov, Georgi R AU - Genov GR AUID- ORCID: 0000-0002-8039-3160 AD - VERAXA Biotech GmbH, Heidelberg, Germany. FAU - Osmanovic, Dina AU - Osmanovic D AD - VERAXA Biotech GmbH, Heidelberg, Germany. FAU - Basic, Ines AU - Basic I AD - VERAXA Biotech GmbH, Heidelberg, Germany. FAU - Koehler, Christine AU - Koehler C AD - VERAXA Biotech GmbH, Heidelberg, Germany. FAU - Sauter, Paul F AU - Sauter PF AUID- ORCID: 0009-0004-5184-9300 AD - VERAXA Biotech GmbH, Heidelberg, Germany. LA - eng GR - VERAXA Biotech GmbH/ PT - Journal Article DEP - 20261029 PL - Germany TA - Angew Chem Int Ed Engl JT - Angewandte Chemie (International ed. in English) JID - 0370543 SB - IM OTO - NOTNLM OT - antibody-drug conjugates OT - bioorthogonal chemistry OT - genetic code expansion OT - trans-cyclooctene EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 03:53 PHST- 2026/09/20 00:00 [revised] PHST- 2026/06/23 00:00 [received] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:53 [entrez] AID - e5717042 [pii] AID - 10.1002/anie.5717042 [doi] PST - aheadofprint SO - Angew Chem Int Ed Engl. 2026 Oct 29:e5717042. doi: 10.1002/anie.5717042. PMID- 42952359 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1399-3054 (Electronic) IS - 0031-9317 (Print) IS - 0031-9317 (Linking) VI - 178 IP - 6 DP - 2026 Nov-Dec TI - Adjustment of Root System Architecture and Root Function in Response to Multifactorial Abiotic Stress. LID - e71170 [pii] LID - 10.1111/ppl.71170 [doi] AB - The root of land plants is a remarkably adaptive organ. Unlike the shoot with its main functions in light harvesting and photosynthesis, sexual reproduction and diaspore distribution, the root explores the soil for nutrient resources and water, while preferably avoiding zones of unfavorable conditions. Roots realize an extraordinarily flexible architecture and perceive and integrate environmental input parameters to optimize growth, architecture, and physiology. In nature, roots encounter a heterogeneous rooting environment characterized by combinations of negative and positive growth parameters, for example, nutrient availability, temperature, hypoxia, toxic metal ions, or compacted soil, necessitating research on multifactorial stress effects on roots. While many studies have addressed the combination of altered nutrient status, in particular nitrogen and phosphorus, and other environmental stress factors such as drought, temperature, and soil compaction, fewer studies addressed climate change-related combinations of stressors such as heat, drought, and hypoxia. The decisive mechanism of environmental input integration targets root growth and architecture, but also exudation. The adjustment is realized by altered activity of apical meristems for root elongation, the pericycle for lateral root initiation and formation, the development of adventitious roots, and root thickening by cambial activity and senescence. This review presents selective case studies to exemplarily summarize current experimental approaches and obtained knowledge on root responses to multifactorial environmental constraints with focus on assimilate-, redox-, and auxin-dependent regulation. The review pinpoints the need to advance and harmonize our experimental approaches by investigating the sensory role and growth response of roots at the local and systemic level. CI - (c) 2026 The Author(s). Physiologia Plantarum published by John Wiley & Sons Ltd on behalf of Scandinavian Plant Physiology Society. FAU - Dietz, Karl-Josef AU - Dietz KJ AUID- ORCID: 0000-0003-0311-2182 AD - Biochemistry and Physiology of Plants, Faculty of Biology, Bielefeld University, Bielefeld, Germany. AD - CeBiTec, Bielefeld University, Bielefeld, Germany. LA - eng GR - DI 346/23-1/Deutsche Forschungsgemeinschaft/ PT - Journal Article PT - Review PL - Denmark TA - Physiol Plant JT - Physiologia plantarum JID - 1256322 RN - 0 (Indoleacetic Acids) SB - IM MH - *Plant Roots/physiology/anatomy & histology MH - *Stress, Physiological/physiology MH - Indoleacetic Acids/metabolism MH - Droughts PMC - PMC13717286 OTO - NOTNLM OT - abiotic stress OT - acclimation OT - cell signaling OT - multifactorial stress OT - root architecture OT - root growth COIS- The paper and the figures are designed without the use of generative AI. The author declares no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/29 CRDT- 2026/10/29 03:43 PHST- 2026/10/09 00:00 [revised] PHST- 2026/06/19 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:43 [entrez] PHST- 2026/10/29 00:00 [pmc-release] AID - e71170 [pii] AID - PPL71170 [pii] AID - 10.1111/ppl.71170 [doi] PST - ppublish SO - Physiol Plant. 2026 Nov-Dec;178(6):e71170. doi: 10.1111/ppl.71170. PMID- 42952347 OWN - NLM STAT- Publisher LR - 20261029 IS - 1613-6829 (Electronic) IS - 1613-6810 (Linking) DP - 2026 Oct 29 TI - Non-Invasive Smart Pressure Sensor for Early-Stage Thermal Runaway Warning for Cylindrical Batteries. LID - e76428 [pii] LID - 10.1002/smll.76428 [doi] AB - Batteries have been widely applied in various fields due to the advantages such as high energy density, long service life and so on, whereas they are accompanied by frequent thermal runaway incidents and this is a worldwide challenge. However, non-invasive warning methods capable of providing early-stage alerts are missing, especially for non-planar sidewall pressure monitoring of cylindrical batteries. Here, a smart pressure sensor based on shape memory sensing composite is introduced, which enables conformal contact with cylindrical batteries and can continuously monitor the lateral pressure caused by volume expansion during charging-discharging. Moreover, the assembly process, designed based on COMSOL simulations, ensures conformal contact between the sensor and the test interface, effectively preventing uneven stress distribution during the assembly of the multi-layer structure. This smart sensor features impressive stability and recoverability, a broad pressure detection range (50-2340 kPa), excellent hysteresis error (1.17%), high linearity (R(2) = 0.99) and a maximum deviation of 1.56% over 20000 cycles. It can monitor radial pressure changes, achieving a warning time ratio of 80%, 84%, 86%, and 79% in thermal runaway tests on 18650, 21700 ternary lithium-ion batteries, 32700 LiFePO(4) battery and 18650 sodium-ion battery, respectively. Timely measures after warning can prevent thermal runaway. CI - (c) 2026 The Author(s). Small published by Wiley-VCH GmbH. FAU - Liu, Yi AU - Liu Y AD - School of Materials Science and Engineering, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Wang, Ang AU - Wang A AD - Institute of Thermal Science and Technology, Shandong University, Jinan, China. AD - Institute for Advanced Technology, Shandong University, Jinan, China. FAU - Xu, Yingying AU - Xu Y AD - Institute of Power Machinery and Vehicular Engineering, College of Energy Engineering, Zhejiang University, Hangzhou, China. FAU - Wu, Yaobo AU - Wu Y AD - Institute of Power Machinery and Vehicular Engineering, College of Energy Engineering, Zhejiang University, Hangzhou, China. FAU - Yang, Shaohua AU - Yang S AD - School of Aeronautics and Astronautics, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Zou, Youyun AU - Zou Y AD - School of Aeronautics and Astronautics, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Wang, Xinyu AU - Wang X AUID- ORCID: 0000-0003-4143-334X AD - Institute of Thermal Science and Technology, Shandong University, Jinan, China. AD - Institute for Advanced Technology, Shandong University, Jinan, China. FAU - Huang, Yuqi AU - Huang Y AUID- ORCID: 0000-0003-3152-5021 AD - Institute of Power Machinery and Vehicular Engineering, College of Energy Engineering, Zhejiang University, Hangzhou, China. FAU - Wang, Zongrong AU - Wang Z AUID- ORCID: 0000-0003-4588-8640 AD - School of Aeronautics and Astronautics, Zhejiang University, Hangzhou, Zhejiang, China. FAU - Qu, Shaoxing AU - Qu S AUID- ORCID: 0000-0002-1217-4644 AD - School of Aeronautics and Astronautics, Zhejiang University, Hangzhou, Zhejiang, China. LA - eng GR - 12372166/National Natural Science Foundation of China/ GR - 12202382/National Natural Science Foundation of China/ GR - LZ23A020005/Natural Science Foundation of Zhejiang Province/ GR - 2019YFC1520900/National Key Research and Development Program of China/ GR - Huanjiang Laboratory/ GR - 226-2023-00002/Fundamental Research Funds for the Central Universities/ GR - 226202400128/Fundamental Research Funds for the Central Universities/ PT - Journal Article DEP - 20261029 PL - Germany TA - Small JT - Small (Weinheim an der Bergstrasse, Germany) JID - 101235338 SB - IM OTO - NOTNLM OT - cylindrical batteries OT - flexible pressure sensor OT - pressure monitoring OT - shape memory polyimide OT - thermal runaway warning EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 03:33 PHST- 2026/10/02 00:00 [revised] PHST- 2026/05/30 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:33 [entrez] AID - e76428 [pii] AID - 10.1002/smll.76428 [doi] PST - aheadofprint SO - Small. 2026 Oct 29:e76428. doi: 10.1002/smll.76428. PMID- 42952341 OWN - NLM STAT- Publisher LR - 20261029 IS - 1521-4095 (Electronic) IS - 0935-9648 (Linking) DP - 2026 Oct 29 TI - Synthesis of Ti(2)B(2)Cl(x) MBenes in Molten Salts From Theoretical and Experimental Perspectives. LID - e75478 [pii] LID - 10.1002/adma.75478 [doi] AB - The unique properties and application possibilities of two-dimensional (2D) materials motivate the exploration of different nanolaminated compounds. Here, by using a molten salt approach, we selectively etch Ti(2)InB(2) with ZnCl(2) to produce a multilayer (ml) Ti(2)B(2)Cl(x) MBene. Upon etching, indium atoms are completely removed from the precursor and replaced by chlorine surface terminations with a coverage of 1.1 < x < 1.4. Further, in situ x-ray diffraction indicates a direct biphasic transformation from Ti(2)InB(2) to ml-MBene, with no signs of intermediate phase formation. A computational framework based on density functional theory further corroborates these experimental observations by showing a negative reaction free energy for the formation of ml-MBene, favourable over all competing processes. In addition, A-element substitution into the 3D Ti(2)ZnB(2) phase is predicted to be endergonic, consistent with the absence of experimental evidence for its formation. Initial Li-ion battery performance evaluation showed a stable discharge capacity similar to or better than MAX phases and other borides. Altogether, the theoretical framework combined with materials synthesis and characterization provides a general approach for 2D materials development, for further expansion of the family of 2D materials. CI - (c) 2026 The Author(s). Advanced Materials published by Wiley-VCH GmbH. FAU - Ronchi, Rodrigo M AU - Ronchi RM AUID- ORCID: 0000-0001-6508-3124 AD - Materials Design division, Department of Physics, Chemistry, and Biology (IFM), Linkoping University, Linkoping, Sweden. FAU - Defoy, Emile AU - Defoy E AUID- ORCID: 0000-0003-4116-1962 AD - Sorbonne Universite, CNRS, Laboratoire de Chimie de La Matiere Condensee de Paris (LCMCP), Paris, France. FAU - Petruhins, Andrejs AU - Petruhins A AUID- ORCID: 0000-0002-9745-5380 AD - Materials Design division, Department of Physics, Chemistry, and Biology (IFM), Linkoping University, Linkoping, Sweden. FAU - Palisaitis, Justinas AU - Palisaitis J AUID- ORCID: 0000-0003-3203-7935 AD - Thin Film Physics Division, Department of Physics, Chemistry, and Biology (IFM), Linkoping University, Linkoping, Sweden. FAU - Qin, Leiqiang AU - Qin L AUID- ORCID: 0000-0002-7047-0927 AD - Materials Design division, Department of Physics, Chemistry, and Biology (IFM), Linkoping University, Linkoping, Sweden. FAU - Halim, Joseph AU - Halim J AUID- ORCID: 0000-0002-7502-1215 AD - Materials Design division, Department of Physics, Chemistry, and Biology (IFM), Linkoping University, Linkoping, Sweden. FAU - Yu, Lianghao AU - Yu L AUID- ORCID: 0009-0007-2952-3541 AD - Materials Design division, Department of Physics, Chemistry, and Biology (IFM), Linkoping University, Linkoping, Sweden. FAU - Tang, Lan AU - Tang L AD - School of Mechanics and Optoelectronic Physics, Anhui University of Science and Technology, Huainan, China. FAU - Reguer, Solenn AU - Reguer S AUID- ORCID: 0000-0002-7449-0503 AD - Synchrotron SOLEIL, L'Orme des Merisiers, Gif-sur-Yvette, France. FAU - Thiaudiere, Dominique AU - Thiaudiere D AD - Synchrotron SOLEIL, L'Orme des Merisiers, Gif-sur-Yvette, France. FAU - Chen, Ningjun AU - Chen N AD - Materials Design division, Department of Physics, Chemistry, and Biology (IFM), Linkoping University, Linkoping, Sweden. FAU - Vavilapalli, Durga Sankar AU - Vavilapalli DS AUID- ORCID: 0000-0002-9456-0230 AD - Materials Design division, Department of Physics, Chemistry, and Biology (IFM), Linkoping University, Linkoping, Sweden. FAU - Portehault, David AU - Portehault D AUID- ORCID: 0000-0003-4914-4913 AD - Sorbonne Universite, CNRS, Laboratoire de Chimie de La Matiere Condensee de Paris (LCMCP), Paris, France. FAU - Bjork, Jonas AU - Bjork J AUID- ORCID: 0000-0002-1345-0006 AD - Materials Design division, Department of Physics, Chemistry, and Biology (IFM), Linkoping University, Linkoping, Sweden. FAU - Persson, Per O A AU - Persson POA AUID- ORCID: 0000-0001-9140-6724 AD - Thin Film Physics Division, Department of Physics, Chemistry, and Biology (IFM), Linkoping University, Linkoping, Sweden. FAU - Rosen, Johanna AU - Rosen J AUID- ORCID: 0000-0002-5173-6726 AD - Materials Design division, Department of Physics, Chemistry, and Biology (IFM), Linkoping University, Linkoping, Sweden. LA - eng GR - 2023.0250/Knut and Alice Wallenberg Foundation/ GR - 2020.0033/Knut and Alice Wallenberg Foundation/ GR - 2022-06725/Swedish Research Council/ GR - 2021-00171/Swedish Research Council/ GR - ERCMULTI2D101087713/ERC_/European Research Council/International GR - ERCGENESIS864850/ERC_/European Research Council/International GR - 864850/ERC_/European Research Council/International GR - SFO-Mat-LiU2009-00971/Swedish Government Strategic Research Area in Materials Science on Advanced Functional Materials at Linkoping University/ GR - MADNESS23-PEXD-0011/National Agency for Research (ANR)/ GR - RIF21-0026/Swedish Foundation for Strategic Research (SSF)/ GR - 20240574/SOLEIL/ PT - Journal Article DEP - 20261029 PL - Germany TA - Adv Mater JT - Advanced materials (Deerfield Beach, Fla.) JID - 9885358 SB - IM OTO - NOTNLM OT - 2D materials OT - DFT OT - MAB phase OT - MBene OT - in situ XRD OT - molten salt EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 03:33 PHST- 2026/10/05 00:00 [revised] PHST- 2026/03/02 00:00 [received] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:33 [entrez] AID - e75478 [pii] AID - 10.1002/adma.75478 [doi] PST - aheadofprint SO - Adv Mater. 2026 Oct 29:e75478. doi: 10.1002/adma.75478. PMID- 42952328 OWN - NLM STAT- Publisher LR - 20261029 IS - 1097-0347 (Electronic) IS - 1043-3074 (Linking) DP - 2026 Oct 29 TI - Response-Adapted Surgery Following Neoadjuvant Chemotherapy in Oral Cavity Squamous Cell Carcinoma: An Era-Based Retrospective Cohort Study. LID - 10.1002/hed.70532 [doi] AB - BACKGROUND: Evidence regarding response-adapted resection after neoadjuvant chemotherapy (NACT) in oral cavity squamous cell carcinoma remains limited. METHODS: We compared conventional-era (2018-2021) and response-adapted-era (2022-2024) surgery after NACT. Margins, recurrence, 2-year survival, and surgical outcomes were assessed. RESULTS: Among 129 patients (58 conventional; 71 response-adapted), positive margins occurred in 3.4% versus 1.4% and local recurrence in 12.1% versus 7.0%. Two-year disease-free survival (DFS) was 56.6% versus 67.8% (log-rank p = 0.135). Adjusted DFS HR for the response-adapted era was 0.54 (95% CI 0.28-1.04; p = 0.066). ENE predicted inferior DFS (HR 3.60; p < 0.001). Responder-only analysis showed no significant survival difference. CONCLUSIONS: No observed increase in positive margins or local recurrence accompanied the later surgical strategy. Retrospective selection, unequal follow-up, and limited events preclude conclusions of noninferiority or a causal survival benefit. CI - (c) 2026 Wiley Periodicals LLC. FAU - Rai, Shreya AU - Rai S AUID- ORCID: 0000-0001-5202-0095 AD - Department of Surgical Oncology, Rajiv Gandhi Cancer Institute and Research Centre, New Delhi, India. FAU - Agarwal, Mudit AU - Agarwal M AD - Department of Surgical Oncology, Rajiv Gandhi Cancer Institute and Research Centre, New Delhi, India. FAU - Singh, Abhishek AU - Singh A AUID- ORCID: 0000-0001-9705-087X AD - Department of Surgical Oncology, Rajiv Gandhi Cancer Institute and Research Centre, New Delhi, India. FAU - Singh, Pradyumna AU - Singh P AD - Department of Surgical Oncology, Rajiv Gandhi Cancer Institute and Research Centre, New Delhi, India. FAU - Bansal, Kshitiz AU - Bansal K AD - Department of Surgical Oncology, Rajiv Gandhi Cancer Institute and Research Centre, New Delhi, India. FAU - Karthik, P AU - Karthik P AD - Department of Surgical Oncology, Rajiv Gandhi Cancer Institute and Research Centre, New Delhi, India. FAU - Gugliani, Abhishek AU - Gugliani A AD - Department of Surgical Oncology, Rajiv Gandhi Cancer Institute and Research Centre, New Delhi, India. FAU - Garg, Neha AU - Garg N AUID- ORCID: 0009-0008-7194-6019 AD - Department of Surgical Oncology, Rajiv Gandhi Cancer Institute and Research Centre, New Delhi, India. FAU - Leela, P AU - Leela P AD - Department of Surgical Oncology, Rajiv Gandhi Cancer Institute and Research Centre, New Delhi, India. FAU - Tandon, Sarthak AU - Tandon S AUID- ORCID: 0000-0002-2319-0391 AD - Department of Radiation Oncology, Rajiv Gandhi Cancer Institute and Research Centre, New Delhi, India. FAU - Goyal, Jitin AU - Goyal J AD - Department of Radiology, Rajiv Gandhi Cancer Institute and Research Centre, New Delhi, India. FAU - Gupta, Manoj AU - Gupta M AD - Department of Nuclear Medicine, Rajiv Gandhi Cancer Institute and Research Centre, New Delhi, India. FAU - Bansal, Rashmi AU - Bansal R AD - Department of Medical Oncology, Rajiv Gandhi Cancer Institute and Research Centre, New Delhi, India. FAU - Saha, Rajat AU - Saha R AD - Department of Medical Oncology, Rajiv Gandhi Cancer Institute and Research Centre, New Delhi, India. FAU - Ahlawat, Parveen AU - Ahlawat P AD - Department of Radiation Oncology, Rajiv Gandhi Cancer Institute and Research Centre, New Delhi, India. FAU - Pasricha, Sunil AU - Pasricha S AUID- ORCID: 0000-0002-5655-5107 AD - Department of OncoPathology, Rajiv Gandhi Cancer Institute and Research Centre, New Delhi, India. FAU - Gairola, Munish AU - Gairola M AD - Department of Radiation Oncology, Rajiv Gandhi Cancer Institute and Research Centre, New Delhi, India. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Head Neck JT - Head & neck JID - 8902541 SB - IM OTO - NOTNLM OT - neoadjuvant chemotherapy OT - oral cavity squamous cell carcinoma OT - propensity score matching OT - surgical margins OT - survival EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 03:23 PHST- 2026/10/04 00:00 [revised] PHST- 2026/05/21 00:00 [received] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:23 [entrez] AID - 10.1002/hed.70532 [doi] PST - aheadofprint SO - Head Neck. 2026 Oct 29. doi: 10.1002/hed.70532. PMID- 42952325 OWN - NLM STAT- Publisher LR - 20261029 IS - 1531-7013 (Electronic) IS - 1087-2418 (Linking) DP - 2026 Oct 30 TI - Genetic and genomic medicine across the lung transplant continuum: inherited disease, pharmacogenomics, and molecular surveillance. LID - 10.1097/MOT.0000000000001325 [doi] AB - PURPOSE OF REVIEW: Genetic information is increasingly relevant across the lung transplant continuum. A molecular diagnosis of the recipient's native lung disease can refine candidate evaluation, identify extrapulmonary vulnerability, guide family counseling, and anticipate posttransplant complications. Pharmacogenetic and molecular biomarkers offer opportunities to individualize therapy and allograft surveillance. This review summarizes the clinical utility and limitations of these emerging approaches. RECENT FINDINGS: Recent literature highlights the importance of cystic fibrosis in the era of highly effective cystic fibrosis transmembrane conductance regulator (CFTR) modulators, short telomere syndromes and hereditary pulmonary fibrosis, alpha-1 antitrypsin deficiency, primary ciliary dyskinesia, pharmacogenomics, and molecular surveillance. Among these, recognition of short telomere syndromes before transplantation has the clearest implications for multidisciplinary risk assessment and posttransplant management. CYP3A5 can inform initial tacrolimus dosing, but therapeutic drug monitoring remains indispensable; thiopurine methyltransferase and Nudix hydrolase 15 are actionable when azathioprine is used. Donor-derived cell-free DNA is a promising, noninvasive marker of allograft injury and prognosis, yet it is not specific for rejection and cannot independently replace microbiologic assessment, donor-specific antibody testing, bronchoscopy, or biopsy. SUMMARY: Genomic tools are most useful when applied selectively and interpreted within clinical context. A tiered implementation model offers a practical path for incorporating genomic tools, improving patient outcomes while limiting overinterpretation. CI - Copyright (c) 2026 Wolters Kluwer Health, LLC. All rights reserved. FAU - Katpally, Ram R AU - Katpally RR AD - Department of Internal Medicine, University of Iowa, Carver College of Medicine, Iowa City, Iowa, USA. FAU - Pena, Tahuanty AU - Pena T FAU - Klesney-Tait, Julia AU - Klesney-Tait J LA - eng PT - Journal Article DEP - 20261030 PL - United States TA - Curr Opin Organ Transplant JT - Current opinion in organ transplantation JID - 9717388 SB - IM OTO - NOTNLM OT - cystic fibrosis OT - donor-derived cell-free DNA OT - genetics OT - lung transplantation OT - pharmacogenomics OT - telomere biology disorder EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 03:22 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:22 [entrez] AID - 00075200-990000000-00249 [pii] AID - 10.1097/MOT.0000000000001325 [doi] PST - aheadofprint SO - Curr Opin Organ Transplant. 2026 Oct 30. doi: 10.1097/MOT.0000000000001325. PMID- 42952322 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1097-0258 (Electronic) IS - 0277-6715 (Linking) VI - 45 IP - 25-27 DP - 2026 Nov TI - Bivariate Copula Models With Event-Free Cure Effect for Survival Extrapolations and Individual-Level Correlation Analysis. LID - e70763 [pii] LID - 10.1002/sim.70763 [doi] AB - OBJECTIVES: Overall survival (OS) extrapolations from mixture cure models (MCMs) are often sensitive to the cure fraction, which is challenging to estimate when data are immature. Furthermore, in some cancer populations, it may be unreasonable to assume curative effects among patients who are not event-free. We addressed these problems using bivariate copula MCMs to jointly model OS and progression- (PFS) or recurrence-free survival (RFS), with a single event-free cure fraction parameter. METHODS: We proposed a practical framework for parametric survival copula model selection and interpretation, which involves estimating five representative candidate copula functions and examining OS distributions conditioned on intermediate event times. Bivariate MCMs with event-free cure fraction, based on previously selected marginal distributions, were estimated for two studies of nivolumab with contrasting survival patterns, namely, in previously treated advanced esophageal squamous cell carcinoma (OS-PFS correlation) and resected melanoma (OS-RFS correlation). RESULTS: OS extrapolations from bivariate MCMs with an effective PFS (or RFS) cure fraction were more conservative than from univariate MCMs. Although patient-level OS-PFS/RFS correlation coefficients were sensitive to the copula, model averaging and inspecting estimated conditional OS distributions enabled clinically meaningful inference on the prognostic impact of intermediate events. CONCLUSIONS: Bivariate MCMs with event-free cured subpopulation incorporated curative effects into OS extrapolations more cautiously than conventional MCMs, based on a more rapidly maturing composite endpoint. The model is especially useful when OS data are immature and if post-event cure is clinically implausible. Employing alternative copulas that cover archetypal association patterns mitigated model selection bias in patient-level correlation analysis. CI - (c) 2026 John Wiley & Sons Ltd. FAU - Sharpe, Daniel J AU - Sharpe DJ AUID- ORCID: 0000-0003-2079-2677 AD - Parexel, London, UK. FAU - Yates, Georgia AU - Yates G AD - Parexel, London, UK. FAU - De, Tuli AU - De T AD - Parexel, Billerica, Massachusetts, USA. FAU - Kandikatla, Renuka AU - Kandikatla R AUID- ORCID: 0009-0003-1353-9670 AD - Bristol-Myers Squibb, Lawrenceville, New Jersey, USA. FAU - Singh, Prianka AU - Singh P AD - Bristol-Myers Squibb, Lawrenceville, New Jersey, USA. FAU - Dixon, Matthew AU - Dixon M AD - Bristol-Myers Squibb, Lawrenceville, New Jersey, USA. FAU - Dave, Kiran AU - Dave K AD - Bristol-Myers Squibb, Uxbridge, UK. FAU - Vanderpuye-Orgle, Jackie AU - Vanderpuye-Orgle J AD - Parexel, Billerica, Massachusetts, USA. FAU - Kurt, Murat AU - Kurt M AUID- ORCID: 0009-0008-9496-6215 AD - Bristol-Myers Squibb, Lawrenceville, New Jersey, USA. LA - eng GR - Bristol-Myers Squibb/ PT - Journal Article PL - England TA - Stat Med JT - Statistics in medicine JID - 8215016 SB - IM MH - Humans MH - *Models, Statistical MH - Survival Analysis MH - Disease-Free Survival MH - Carcinoma, Squamous Cell/drug therapy/mortality MH - Melanoma/drug therapy/mortality/surgery MH - Esophageal Neoplasms/drug therapy/mortality MH - Progression-Free Survival MH - Computer Simulation OTO - NOTNLM OT - copula models OT - cure model OT - individual-level correlation analysis OT - surrogate outcomes analysis OT - survival analysis OT - survival extrapolations EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 CRDT- 2026/10/29 03:22 PHST- 2026/08/16 00:00 [revised] PHST- 2025/03/28 00:00 [received] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:22 [entrez] AID - e70763 [pii] AID - 10.1002/sim.70763 [doi] PST - ppublish SO - Stat Med. 2026 Nov;45(25-27):e70763. doi: 10.1002/sim.70763. PMID- 42952319 OWN - NLM STAT- Publisher LR - 20261029 IS - 2234-2400 (Print) IS - 2234-2400 (Linking) DP - 2026 Oct 14 TI - Comparison of the early double-wire technique with the repeat single-wire technique for successful bile duct cannulation: a systematic review and meta-analysis. LID - 10.5946/ce.2025.373 [doi] AB - BACKGROUND/AIMS: Inadvertent pancreatic duct cannulation during endoscopic retrograde cholangiopancreatography (ERCP) presents a challenge in selective bile duct cannulation. Potential strategies include early double-guidewire (EDG) and repeated single-guidewire (RSG) techniques, though comparative effectiveness remains unclear. To evaluate the efficacy and safety of EDG versus RSG in patients with unintentional pancreatic duct cannulation during ERCP for biliary interventions. METHODS: We conducted a systematic review and meta-analysis of randomized controlled trials and cohort studies comparing EDG and RSG from Cochrane Central Register of Controlled Trials, Embase, and Ovid Medline until February 25, 2025. The primary outcome was successful bile duct cannulation. Secondary outcomes included rate of post-ERCP pancreatitis (PEP). RESULTS: Three studies comprising 464 patients (228 EDG, 236 RSG) were included. The pooled risk ratio for successful biliary cannulation favored EDG (1.38; 95% confidence interval [CI], 0.96-1.99; p=0.08). Rates of PEP were similar between groups (risk ratio, 1.12; 95% CI, 0.69-1.82; p=0.64). Subgroup analyses suggested greater benefit of EDG when performed by expert endoscopists (risk ratio, 1.42; 95% CI, 1.05-1.91, p=0.02). CONCLUSIONS: EDG may improve cannulation success in selected populations, particularly under expert operators, without increasing PEP risk. Considerable heterogeneity underscores the need for further large-scale studies to clarify its role. FAU - Zheng, Katina AU - Zheng K AD - Division of Gastroenterology, St. Michael's Hospital, University of Toronto, Toronto, ON, Canada. FAU - Alabdulkarim, Balqis AU - Alabdulkarim B AD - Division of Gastroenterology, St. Michael's Hospital, University of Toronto, Toronto, ON, Canada. FAU - Khalaf, Kareem AU - Khalaf K AD - Division of Gastroenterology, St. Michael's Hospital, University of Toronto, Toronto, ON, Canada. FAU - Yuan, Yuhong AU - Yuan Y AD - Department of Medicine, McMaster University, Hamilton, ON, Canada. AD - Department of Medicine, Western University, London, ON, Canada. FAU - Bucheeri, Mohamed Abdulla Ghuloom Abdulla AU - Bucheeri MAGA AD - Division of Gastroenterology, St. Michael's Hospital, University of Toronto, Toronto, ON, Canada. FAU - Li, Huaqi AU - Li H AD - Division of Gastroenterology, St. Michael's Hospital, University of Toronto, Toronto, ON, Canada. FAU - Teshima, Christopher AU - Teshima C AD - Division of Gastroenterology, St. Michael's Hospital, University of Toronto, Toronto, ON, Canada. FAU - May, Gary Robert AU - May GR AD - Division of Gastroenterology, St. Michael's Hospital, University of Toronto, Toronto, ON, Canada. FAU - Mosko, Jeffrey D AU - Mosko JD AD - Division of Gastroenterology, St. Michael's Hospital, University of Toronto, Toronto, ON, Canada. FAU - Calo, Natalia Causada AU - Calo NC AD - Division of Gastroenterology, St. Michael's Hospital, University of Toronto, Toronto, ON, Canada. LA - eng PT - Journal Article DEP - 20261014 PL - Korea (South) TA - Clin Endosc JT - Clinical endoscopy JID - 101576886 OTO - NOTNLM OT - Bile ducts OT - Catheterization OT - Endoscopic retrograde cholangiopancreatography OT - Pancreatic ducts OT - Pancreatitis EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 03:20 PHST- 2025/10/15 00:00 [received] PHST- 2026/04/08 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:20 [entrez] AID - ce.2025.373 [pii] AID - 10.5946/ce.2025.373 [doi] PST - aheadofprint SO - Clin Endosc. 2026 Oct 14. doi: 10.5946/ce.2025.373. PMID- 42952316 OWN - NLM STAT- Publisher LR - 20261029 IS - 2234-2400 (Print) IS - 2234-2400 (Linking) DP - 2026 Oct 29 TI - Navigating the safety recall of selected Hot-AXIOS sizes: international survey of practice adaptations among therapeutic endoscopic ultrasound experts. LID - 10.5946/ce.2026.271 [doi] FAU - Vanella, Giuseppe AU - Vanella G AD - Pancreatobiliary Endoscopy and Endosonography Division, IRCCS San Raffaele Scientific Institute, Milan, Italy. AD - Vita-Salute San Raffaele University, Milan, Italy. FAU - Marzocca, Giuseppe AU - Marzocca G AD - Pancreatobiliary Endoscopy and Endosonography Division, IRCCS San Raffaele Scientific Institute, Milan, Italy. AD - Vita-Salute San Raffaele University, Milan, Italy. FAU - Bronswijk, Michiel AU - Bronswijk M AD - University Hospitals Gasthuisberg, University of Leuven, Leuven, Belgium. AD - Imelda General Hospital, Bonheiden, Belgium. FAU - Voermans, Rogier P AU - Voermans RP AD - Amsterdam University Medical Center, location University of Amsterdam, Amsterdam, The Netherlands. AD - Cancer Center Amsterdam, Amsterdam, The Netherlands. FAU - van der Merwe, Schalk AU - van der Merwe S AD - University Hospitals Gasthuisberg, University of Leuven, Leuven, Belgium. FAU - Arcidiacono, Paolo Giorgio AU - Arcidiacono PG AD - Pancreatobiliary Endoscopy and Endosonography Division, IRCCS San Raffaele Scientific Institute, Milan, Italy. AD - Vita-Salute San Raffaele University, Milan, Italy. FAU - van Wanrooij, Roy L J AU - van Wanrooij RLJ AD - Cancer Center Amsterdam, Amsterdam, The Netherlands. AD - Amsterdam UMC, location Vrije University, Amsterdam, The Netherlands. CN - AXIOS-RECOVER Group LA - eng PT - Journal Article DEP - 20261029 PL - Korea (South) TA - Clin Endosc JT - Clinical endoscopy JID - 101576886 EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 03:20 PHST- 2026/06/04 00:00 [received] PHST- 2026/08/01 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:20 [entrez] AID - ce.2026.271 [pii] AID - 10.5946/ce.2026.271 [doi] PST - aheadofprint SO - Clin Endosc. 2026 Oct 29. doi: 10.5946/ce.2026.271. PMID- 42952302 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1473-656X (Electronic) IS - 1040-872X (Linking) VI - 38 IP - 6 DP - 2026 Dec 1 TI - Implementing sustainability in gynecologic surgery: barriers and a roadmap for change. PG - 482-488 LID - 10.1097/GCO.0000000000001150 [doi] AB - PURPOSE OF REVIEW: Healthcare generates more than 4% of global greenhouse gas emissions, and the operating room is a disproportionate contributor. Gynecologic surgery is an important target for interventions as a high-volume specialty heavily reliant on disposable trocars, energy devices, and custom packs. Despite growing calls to action and evidence that selected sustainability initiatives can be implemented without compromising patient safety, adoption remains inconsistent and often dependent on local champions. RECENT FINDINGS: Barriers span five domains: (a) Organizational and structural obstacles including limited governance, hierarchical decision-making, inconsistent regulatory guidance, and supply chains favoring single-use products. (b) Knowledge and training gaps persisting across perioperative teams and hospital leadership, compounded by the lack of standardized environmental metrics. (c) Cultural resistance reflecting safety concerns about reusables despite demonstrated clinical equivalence. (d) Operational barriers including inadequate infrastructure and upfront costs preceding potential savings. (e) Intervention-specific challenges that limit implementation of waste segregation, reusable transitions, anesthetic gas management, and energy efficiency. SUMMARY: Addressing these barriers requires leadership commitment, clearer regulatory guidance, ongoing education, industry collaboration, stakeholder engagement, and context-specific application of implementation science and quality-improvement methods. Standardized metrics, institution-specific targets, and ongoing monitoring can support phased implementation, track progress, and build institutional support for sustained change. CI - Copyright (c) 2026 Wolters Kluwer Health, LLC. All rights reserved. FAU - Hall, Evelyn F AU - Hall EF AD - Division of Urogynecology and Reconstructive Pelvic Surgery, Department of Obstetrics and Gynecology, Tufts University School of Medicine and Tufts Medical Center, Boston, Massachusetts. FAU - Dilger, Amanda E AU - Dilger AE AD - Department of Otorhinolaryngology - Head and Neck Surgery, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York, USA. FAU - Mou, Tsung AU - Mou T AD - Division of Urogynecology and Reconstructive Pelvic Surgery, Department of Obstetrics and Gynecology, Tufts University School of Medicine and Tufts Medical Center, Boston, Massachusetts. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - England TA - Curr Opin Obstet Gynecol JT - Current opinion in obstetrics & gynecology JID - 9007264 SB - IM MH - Humans MH - *Gynecologic Surgical Procedures/standards/instrumentation MH - Female MH - *Operating Rooms/organization & administration OTO - NOTNLM OT - carbon footprint OT - environmental sustainability OT - gynecologic surgery OT - implementation science OT - operating room waste EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 CRDT- 2026/10/29 03:13 PHST- 2026/09/17 00:00 [received] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:13 [entrez] AID - 00001703-202612000-00016 [pii] AID - 10.1097/GCO.0000000000001150 [doi] PST - ppublish SO - Curr Opin Obstet Gynecol. 2026 Dec 1;38(6):482-488. doi: 10.1097/GCO.0000000000001150. Epub 2026 Oct 27. PMID- 42952301 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1473-656X (Electronic) IS - 1040-872X (Linking) VI - 38 IP - 6 DP - 2026 Dec 1 TI - The impact of midlife hormonal treatment on lower urinary tract symptoms. PG - 440-445 LID - 10.1097/GCO.0000000000001159 [doi] AB - PURPOSE OF REVIEW: Women in midlife are increasingly interested in receiving menopausal hormone therapy (MHT) for symptom relief. Increased patient interest and MHT prescribing require a clinical understanding of the effect of hormonal treatment on lower urinary tract symptoms (LUTS), which often develop in midlife and worsen with age. This review summarizes the divergent LUTS effects conferred by hormone type and mode of delivery. RECENT FINDINGS: Vaginal estrogen is well established to help alleviate LUTS, and recent data suggest that periurethral application is noninferior to intravaginal application. Many studies have shown that systemic estrogen results in new and worsening LUTS, and recent studies have begun to probe this opposing effect. Selective estrogen receptor modulators are less well studied but seem to have a less deleterious effect on LUTS than MHT. SUMMARY: The mechanism of the divergent effects of estrogen on LUTS by route of administration remains unclear, but further research may help provide more individualized hormone therapy options for women in midlife experiencing systemic and/or LUTS. CI - Copyright (c) 2026 Wolters Kluwer Health, LLC. All rights reserved. FAU - Byrne, Elizabeth H AU - Byrne EH AD - Mass General Brigham Urogynecology and Reconstructive Pelvic Surgery, Boston, Massachusetts, USA. FAU - Von Bargen, Emily C AU - Von Bargen EC LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - England TA - Curr Opin Obstet Gynecol JT - Current opinion in obstetrics & gynecology JID - 9007264 RN - 0 (Estrogens) RN - 0 (Selective Estrogen Receptor Modulators) SB - IM MH - Humans MH - Female MH - *Lower Urinary Tract Symptoms/drug therapy MH - *Estrogen Replacement Therapy/methods/adverse effects MH - *Estrogens/administration & dosage/adverse effects MH - Middle Aged MH - *Menopause MH - Administration, Intravaginal MH - Selective Estrogen Receptor Modulators/administration & dosage/therapeutic use OTO - NOTNLM OT - lower urinary tract symptoms OT - menopausal hormone therapy OT - urinary incontinence OT - vaginal estrogen EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 CRDT- 2026/10/29 03:13 PHST- 2026/08/21 00:00 [received] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:13 [entrez] AID - 00001703-202612000-00009 [pii] AID - 10.1097/GCO.0000000000001159 [doi] PST - ppublish SO - Curr Opin Obstet Gynecol. 2026 Dec 1;38(6):440-445. doi: 10.1097/GCO.0000000000001159. Epub 2026 Oct 27. PMID- 42952297 OWN - NLM STAT- Publisher LR - 20261029 IS - 1521-4095 (Electronic) IS - 0935-9648 (Linking) DP - 2026 Oct 29 TI - Dual Neuromodulation Remodels Neuro-Immune Crosstalk and Chemokine Network for Combating Postoperative Progression of Prostate Cancer. LID - e75452 [pii] LID - 10.1002/adma.75452 [doi] AB - The autonomic nerves drive prostate cancer (PCa) progression, yet a strategy for dual modulation of sympathetic and parasympathetic nerves remains undeveloped. Here, building on the multicenter clinical findings, we develop a personalized nanotechnology-enabled dual neurotherapy (denoted as ADNT) for PCa. The ADNT employs hybrid nanovesicles composed of autologous tumor cell membranes and functionalized liposomes to codeliver the beta-blocker and the muscarinic receptor antagonist to autonomic nerves in residual tumor. In refractory, highly invasive, and orthotopic murine PCa models, the ADNT effectively delays postoperative recurrence and metastasis. Light-sheet fluorescence microscopy visually confirms the downregulation of autonomic nerve infiltration. Cytometry by time-of-flight reveals that ADNT remodels neuro-immune crosstalk and the chemokine network, promoting antitumor immune response and suppressing tumor progression. Additionally, ADNT also alleviates cancer-associated cachexia and anxiety. Combined with immune checkpoint blockade, ADNT induces complete responses in 100% of mice. Immunofluorescence staining of human PCa specimens confirms the clinical translatability of ADNT, establishing ADNT as a promising therapeutic strategy for PCa. CI - (c) 2026 Wiley-VCH GmbH. FAU - Hao, Shi-Jie AU - Hao SJ AD - State Key Laboratory of Digital Medical Engineering, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China. FAU - Zheng, Xiejunhao AU - Zheng X AD - Department of Urology, Zhongda Hospital, Southeast University, Nanjing, P. R. China. AD - Surgical Research Center, Institute of Urology, Medical School of Southeast University, Nanjing, P. R. China. FAU - Wu, Shun-Yu AU - Wu SY AD - State Key Laboratory of Digital Medical Engineering, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China. FAU - Wang, Zitong AU - Wang Z AD - Department of Urology, Zhongda Hospital, Southeast University, Nanjing, P. R. China. AD - Surgical Research Center, Institute of Urology, Medical School of Southeast University, Nanjing, P. R. China. FAU - Wu, Tiange AU - Wu T AD - Department of Urology, Zhongda Hospital, Southeast University, Nanjing, P. R. China. AD - Surgical Research Center, Institute of Urology, Medical School of Southeast University, Nanjing, P. R. China. FAU - An, Yanli AU - An Y AD - Center of Interventional Radiology and Vascular Surgery, Department of Radiology, Medical School, Nurturing Center of Jiangsu Province for State Laboratory of AI Imaging & Interventional Radiology (Southeast University), Basic Medicine Research and Innovation Center of Ministry of Education, Zhongda Hospital, Southeast University, Nanjing, P. R. China. FAU - Guan, Han AU - Guan H AUID- ORCID: 0009-0002-1537-0824 AD - Department of Urology, First Affiliated Hospital of Bengbu Medical College, Bengbu, P. R. China. FAU - Wang, Zihao AU - Wang Z AD - State Key Laboratory of Digital Medical Engineering, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China. FAU - Zhang, Shen AU - Zhang S AD - State Key Laboratory of Digital Medical Engineering, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China. FAU - Yang, Wei AU - Yang W AUID- ORCID: 0009-0007-4898-8694 AD - State Key Laboratory of Digital Medical Engineering, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China. FAU - Teng, Gao-Jun AU - Teng GJ AUID- ORCID: 0000-0002-5951-4538 AD - Cultivation and Construction Site of the State Key Laboratory of Intelligent Imaging and Interventional Medicine, Department of Radiology, Zhongda Hospital, Medical School of Southeast University, Nanjing, P. R. China. FAU - Xu, Bin AU - Xu B AUID- ORCID: 0000-0003-4993-0500 AD - Department of Urology, Zhongda Hospital, Southeast University, Nanjing, P. R. China. AD - National Innovation Platform for Integration of Medical Engineering Education (NMEE), Basic Medicine Research and Innovation Center (Ministry of Education), Southeast University, Nanjing, P. R. China. FAU - Wu, Fu-Gen AU - Wu FG AUID- ORCID: 0000-0003-1773-2868 AD - State Key Laboratory of Digital Medical Engineering, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing, P. R. China. LA - eng GR - 2025ZD0545600/Noncommunicable Chronic Diseases-National Science and Technology Major Project/ GR - 82372127/National Natural Science Foundation of China/ GR - BE2023771/Social Development General Project of Jiangsu Science and Technology Department/ GR - CXJH_SEU 25142/SEU Innovation Capability Enhancement Plan for Doctoral Students/ PT - Journal Article DEP - 20261029 PL - Germany TA - Adv Mater JT - Advanced materials (Deerfield Beach, Fla.) JID - 9885358 SB - IM OTO - NOTNLM OT - autonomic nervous system OT - cachexia OT - cancer nanotechnology OT - immune checkpoint OT - immune system OT - metastasis OT - neuroimmunology OT - prostate cancer OT - taxonomy OT - tumor progression EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 03:13 PHST- 2026/10/06 00:00 [revised] PHST- 2026/07/20 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:13 [entrez] AID - e75452 [pii] AID - 10.1002/adma.75452 [doi] PST - aheadofprint SO - Adv Mater. 2026 Oct 29:e75452. doi: 10.1002/adma.75452. PMID- 42952296 OWN - NLM STAT- Publisher LR - 20261029 IS - 1538-8514 (Electronic) IS - 1535-7163 (Linking) DP - 2026 Oct 29 TI - Proliferating Cell Nuclear Antigen as New Therapeutic Target in Multiple Myeloma. LID - 10.1158/1535-7163.MCT-25-1543 [doi] AB - Multiple myeloma (MM), the second most common hematologic malignancy, remains incurable despite major therapeutic advances, highlighting an urgent need for novel therapeutic strategies. PCNA is involved in DNA replication and repair, chromatin remodeling, and cell-cycle progression, and increased PCNA expression has been associated with aggressive MM phenotypes and poor clinical outcomes. Cancer cells express a post-translationally modified cancer-associated isoform of PCNA (caPCNA), potentially providing a selective therapeutic target. We evaluated the anti-myeloma activity of the novel caPCNA inhibitor AOH1996 alone and in combination with established anti-myeloma agents in vitro, in vivo, and ex vivo. AOH1996 inhibited growth of multiple MM cell lines in a dose-dependent manner, induced G2-phase accumulation and apoptosis, and reduced sphere formation and colony formation capacity. Combination treatment with bortezomib, venetoclax, melphalan, or cyclophosphamide enhanced anti-myeloma activity relative to single-agent treatment. AOH1996 treatment down-regulated the expression of multiple tumor-associated proteins and inflammatory and pro-survival cytokines. miRNA analysis demonstrated suppression of several oncogenic miRNAs along with upregulation of tumor-suppressive miRNA miR-145. Forced expression of miR-145 inhibited MM cell growth and enhanced sensitivity to AOH1996, implicating miR-145 as a mechanistic mediator of PCNA inhibition's anti-tumor effects. In xenograft models, AOH1996 significantly enhanced the anti-tumor activity of bortezomib and venetoclax without apparent toxicity. Ex vivo studies demonstrated selective activity against primary MM cells and CD138+ plasma cells with minimal effects on normal PBMCs. Collectively, these findings support caPCNA as a promising therapeutic target in MM and provide strong preclinical rationale for further clinical investigation of AOH1996-based therapeutic strategies. FAU - Bao, Bin AU - Bao B AUID- ORCID: 0000-0002-9627-0392 AD - Wayne State University Detroit, MI United States. ROR: https://ror.org/01070mq45 FAU - Aboukameel, Amro AU - Aboukameel A AUID- ORCID: 0009-0009-9912-2887 AD - Wayne State University United States. ROR: https://ror.org/01070mq45 FAU - Bannoura, Sahar F AU - Bannoura SF AUID- ORCID: 0009-0008-1832-902X AD - Wayne State University Detroit, MI United States. ROR: https://ror.org/01070mq45 FAU - Khan, Husain Yar AU - Khan HY AUID- ORCID: 0000-0002-9161-1514 AD - Wayne State University Detroit, MI United States. ROR: https://ror.org/01070mq45 FAU - Uddin, Md Hafiz AU - Uddin MH AUID- ORCID: 0000-0002-0188-6857 AD - Wayne State University Detroit, MI United States. ROR: https://ror.org/01070mq45 FAU - Shi, Yang AU - Shi Y AUID- ORCID: 0000-0002-3993-2599 AD - The Barbara Ann Karmanos Cancer Institute Detroit, Michigan United States. ROR: https://ror.org/00ee40h97 FAU - Wan, Yin AU - Wan Y AUID- ORCID: 0000-0001-5455-105X AD - Wayne State University Detroit, MI United States. FAU - Sukkari, M Walid AU - Sukkari MW AUID- ORCID: 0000-0002-2545-0142 AD - Wayne State University Detroit, MI United States. ROR: https://ror.org/01070mq45 FAU - Mohammad, Ramzi M AU - Mohammad RM AUID- ORCID: 0000-0003-0232-9101 AD - Wayne State University School of Medicine, Karmanos Cancer Institute Detroit, Michigan United States. FAU - Kaddoura, Marcella AU - Kaddoura M AUID- ORCID: 0000-0002-4529-7405 AD - Wayne State University Detroit, MI United States. ROR: https://ror.org/01070mq45 FAU - Kin, Andrew AU - Kin A AUID- ORCID: 0000-0002-3263-2374 AD - Wayne State University Detroit, MI United States. ROR: https://ror.org/01070mq45 FAU - Cole, Craig E AU - Cole CE AUID- ORCID: 0000-0002-5537-761X AD - The Barbara Ann Karmanos Cancer Institute Detroit, MI United States. ROR: https://ror.org/00ee40h97 FAU - Sucheston-Campbell, Lara AU - Sucheston-Campbell L AUID- ORCID: 0009-0002-7403-5559 AD - Wayne State University Detroit, MI United States. ROR: https://ror.org/01070mq45 FAU - Gu, Long AU - Gu L AUID- ORCID: 0000-0002-3536-5056 AD - Beckman Research Institute of City of Hope Duarte, CA United States. FAU - Haratipour, Pouya AU - Haratipour P AUID- ORCID: 0000-0003-0181-8958 AD - City Of Hope National Medical Center United States. ROR: https://ror.org/00w6g5w60 FAU - Hickey, Robert J AU - Hickey RJ AUID- ORCID: 0000-0001-5099-8137 AD - Beckman Research Institute of City of Hope Duarte, CA United States. FAU - Malkas, Linda H AU - Malkas LH AUID- ORCID: 0000-0001-7624-8510 AD - City of Hope Duarte, CA United States. ROR: https://ror.org/01z1vct10 FAU - Azmi, Asfar S AU - Azmi AS AUID- ORCID: 0000-0003-1178-9505 AD - Wayne State University Detroit, MI United States. ROR: https://ror.org/01070mq45 FAU - Zonder, Jeffrey A AU - Zonder JA AUID- ORCID: 0000-0002-1741-2795 AD - Wayne State University Detroit, MI United States. ROR: https://ror.org/01070mq45 LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Mol Cancer Ther JT - Molecular cancer therapeutics JID - 101132535 SB - IM EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 03:13 PHST- 2026/10/15 00:00 [accepted] PHST- 2025/12/26 00:00 [received] PHST- 2026/05/19 00:00 [revised] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:13 [entrez] AID - 789709 [pii] AID - 10.1158/1535-7163.MCT-25-1543 [doi] PST - aheadofprint SO - Mol Cancer Ther. 2026 Oct 29. doi: 10.1158/1535-7163.MCT-25-1543. PMID- 42952293 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1530-6860 (Electronic) IS - 0892-6638 (Print) IS - 0892-6638 (Linking) VI - 40 IP - 21 DP - 2026 Nov 15 TI - Multi-Organ Inflammatory and Endocrine Crosstalk Shapes the Early Brain Response to Combined Burn and Blast Injury. LID - e72354 [pii] LID - 10.1096/fj.202504681R [doi] AB - The pathology of concomitant burn and traumatic brain injury remains poorly defined, despite their frequent co-occurrence in military settings and the potential for deleterious synergy. To address this gap, we combined established models of blast-induced mild traumatic brain injury and 15% total body surface area flame burn to approximate a common combat-related trauma and examined how the injuries interact to shape systemic inflammation and brain transcriptional programs. Burn injury induced systemic G-CSF and IL-6 secretion and hematopoietic reprioritization, whereas overlapping brain injury did not substantially alter these systemic effects but magnified brain transcriptional responses relative to single injuries. Bulk RNA-seq profiling at 6 h post-injury revealed enriched gene sets associated with inflammation and endocrine stress in the combined injury group, alongside elevated systemic cytokines and corticosterone; thereby suggesting concurrent immune-endocrine activation. Since our prior work demonstrated burn wound-associated IL-1 signaling induces G-CSF and IL-6 secretion, we tested IL-1 neutralizing antibodies and anakinra as therapies. Treatment reduced G-CSF and IL-6 secretion, improved erythropoiesis, and selectively modulated brain transcriptional changes. Single-nucleus RNA-seq projection mapping predicted that the transcripts altered by combined injury were broadly enriched across vascular and glial cell types. Targeted expression profiling identified a shared brain-liver signature characterized by an IL-1/6-dependent inflammatory response alongside an interleukin-independent stress response. These findings highlight IL-1-responsive inflammatory signals and endocrine stress interactions within a multi-organ network that shapes the early brain transcriptional response to the combined injury. CI - (c) 2026 The Author(s). The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology. FAU - Schwierling, Hana C AU - Schwierling HC AD - Division of Pharmaceutical Sciences, James L. Winkle College of Pharmacy, University of Cincinnati, Cincinnati, Ohio, USA. FAU - Sengupta, Satarupa AU - Sengupta S AD - Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA. FAU - Noel, John G AU - Noel JG AD - Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA. FAU - O'Connell, Christopher J AU - O'Connell CJ AD - Department of Genetics, Harvard University School of Medicine, Cambridge, Massachusetts, USA. FAU - Potter, Andrew S AU - Potter AS AD - Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA. FAU - Adam, Mike AU - Adam M AD - Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA. FAU - Robson, Matthew J AU - Robson MJ AD - Division of Pharmaceutical Sciences, James L. Winkle College of Pharmacy, University of Cincinnati, Cincinnati, Ohio, USA. FAU - Gardner, Jason C AU - Gardner JC AUID- ORCID: 0000-0002-5924-9201 AD - Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio, USA. LA - eng GR - W81XWH-22-1-0849/Office of the Assistant Secretary of Defense for Health Affairs through the Military Burn Research Program/ GR - RO1HL155579/HHS | National Institutes of Health (NIH)/ GR - R01NS137887/HHS | National Institutes of Health (NIH)/ PT - Journal Article PL - United States TA - FASEB J JT - FASEB journal : official publication of the Federation of American Societies for Experimental Biology JID - 8804484 RN - 0 (Interleukin-6) RN - 143011-72-7 (Granulocyte Colony-Stimulating Factor) RN - W980KJ009P (Corticosterone) SB - IM MH - Animals MH - *Burns/metabolism/complications/pathology MH - *Inflammation/metabolism/pathology MH - *Blast Injuries/metabolism/complications/pathology MH - *Brain/metabolism/pathology MH - *Brain Injuries, Traumatic/metabolism/pathology MH - Male MH - Interleukin-6/metabolism MH - Granulocyte Colony-Stimulating Factor/metabolism MH - Rats MH - Corticosterone/metabolism PMC - PMC13717289 OTO - NOTNLM OT - anemia OT - blast-induced brain injury OT - burn injury OT - hypothalamic-pituitary-adrenal axis OT - interleukin-1 OT - neuroinflammation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/29 CRDT- 2026/10/29 03:12 PHST- 2026/06/01 00:00 [revised] PHST- 2025/12/17 00:00 [received] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:12 [entrez] PHST- 2026/10/29 00:00 [pmc-release] AID - e72354 [pii] AID - FSB272354 [pii] AID - 10.1096/fj.202504681R [doi] PST - ppublish SO - FASEB J. 2026 Nov 15;40(21):e72354. doi: 10.1096/fj.202504681R. PMID- 42952291 OWN - NLM STAT- Publisher LR - 20261029 IS - 1748-3115 (Electronic) IS - 1748-3107 (Linking) DP - 2026 Oct 28 TI - Mobility devices, social inclusion and participation in youth with disabilities: a scoping review. PG - 1-16 LID - 10.1080/17483107.2026.2748804 [doi] AB - OBJECTIVE: To examine how participation and social inclusion are addressed in the literature related to mobility device use among youth with disabilities. MATERIALS & METHODS: A scoping review was conducted using Arksey and O'Malley's five-stage framework. Eight databases, including MEDLINE (OVID), Web of Science, Embase, CINAHL, Emcare, Social Work Abstracts, Social Sciences Abstracts and Sociological Abstracts, were searched for studies examining mobility device use in relation to participation and social inclusion among youth aged 14 to 21 years with childhood-onset motor disabilities. Data were extracted and organised by participation setting. RESULTS: Fourteen articles were included in this review, with the majority focusing on wheelchair use. The findings suggested that mobility devices may support participation across diverse settings, including physical activity, home and social settings. However, there remains a gap in understanding how mobility devices influence social inclusion among youth, because social inclusion was not measured directly as a primary outcome in the included studies. CONCLUSION: Mobility devices may support independence and participation among youth with disabilities across home, school, community and recreational settings. However, their specific role in promoting social inclusion remains unclear. Future research should focus on how different types of mobility devices impact social inclusion. Such evidence may inform clinical decision-making, support device selection and promote youth development, well-being and social integration. FAU - Firouzeh, Pegah AU - Firouzeh P AUID- ORCID: 0000-0002-1578-8887 AD - CanChild Center for Childhood-Onset Disability Research, School of Rehabilitation Science, McMaster University, Hamilton, Canada. FAU - Szilagy, Alexis AU - Szilagy A AD - Faculty of Health Sciences, McMaster University, Hamilton, Canada. FAU - Di Rezze, Briano AU - Di Rezze B AUID- ORCID: 0000-0002-6061-2506 AD - CanChild Center for Childhood-Onset Disability Research, School of Rehabilitation Science, McMaster University, Hamilton, Canada. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - Disabil Rehabil Assist Technol JT - Disability and rehabilitation. Assistive technology JID - 101255937 SB - IM OAB - Mobility device selection should consider functional mobility as well as youths' participation goals, social engagement and preferred activities across home, school and community settings.Environmental barriers and facilitators should be considered when making decisions about mobility devices to ensure they effectively support meaningful participation.Research and clinical practice may benefit from standardised tools for assessing participation and social inclusion among youth who use mobility devices. OABL- eng OTO - NOTNLM OT - Youth with disabilities OT - mobility devices OT - participation OT - scoping review OT - social inclusion EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 03:03 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:03 [entrez] AID - 10.1080/17483107.2026.2748804 [doi] PST - aheadofprint SO - Disabil Rehabil Assist Technol. 2026 Oct 28:1-16. doi: 10.1080/17483107.2026.2748804. PMID- 42952285 OWN - NLM STAT- Publisher LR - 20261029 IS - 1526-3231 (Electronic) IS - 0749-8063 (Linking) DP - 2026 Oct 28 TI - Arthroscopic Capsular Release Provides Greater Range-of-Motion Improvement Within the First 6 Months Postoperatively Than Nonoperative Management Modalities in Frozen Shoulder: A Systematic Review and Meta-analysis of Randomized Controlled Trials. LID - 10.1002/arj.70519 [doi] AB - PURPOSE: To evaluate the comparative efficacy and safety of arthroscopic capsular release (ACR) compared with nonoperative management modality for frozen shoulder. METHODS: A systematic search of PubMed, Embase, Cochrane Library, Web of Science, China National Knowledge Infrastructure, and Wanfang databases was conducted to identify randomized controlled trials comparing ACR with nonoperative management modalities for frozen shoulder. Two reviewers independently performed study selection, data extraction, and quality assessment. Outcomes included shoulder pain assessed using the visual analog scale, shoulder function, range of motion, and complications. RESULTS: The analysis included 6 randomized controlled trials encompassing 705 patients. Evidence of very low to moderate certainty indicated that, within the first 6 months postoperatively, although the improvement in shoulder range of motion with ACR reached the minimal clinically important difference, the magnitude of this improvement diminished over time. Regarding pain, a statistically significant but not clinically meaningful improvement was observed at 6 months postoperatively; no significant improvement was found during other follow-up periods. Safety profiles were comparable between both treatment approaches. CONCLUSIONS: The evidence suggests that ACR may offer superior improvements in shoulder range of motion within the first 6 months postoperatively compared with nonoperative management, but the evidence quality is very low to moderate, primarily because of significant heterogeneity and methodological limitations. LEVEL OF EVIDENCE: Level I, systematic review of Level I studies. CI - (c) 2026 the Arthroscopy Association of North America. FAU - Sun, Zilin AU - Sun Z AUID- ORCID: 0009-0000-5942-6389 AD - School of Physical Education, China University of Geosciences (Wuhan), Wuhan, China. FAU - Liu, Renyi AU - Liu R AUID- ORCID: 0000-0003-4448-5719 AD - School of Physical Education, China University of Geosciences (Wuhan), Wuhan, China. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Arthroscopy JT - Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association JID - 8506498 SB - IM EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 03:02 PHST- 2025/07/09 00:00 [received] PHST- 2026/03/24 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 03:02 [entrez] AID - 10.1002/arj.70519 [doi] PST - aheadofprint SO - Arthroscopy. 2026 Oct 28. doi: 10.1002/arj.70519. PMID- 42952282 OWN - NLM STAT- Publisher LR - 20261029 IS - 1613-6829 (Electronic) IS - 1613-6810 (Linking) DP - 2026 Oct 28 TI - Chitosan-Gated Polycarbonate Nanochannels for Tunable Water and Cation Transport. LID - e76447 [pii] LID - 10.1002/smll.76447 [doi] AB - Biological membranes regulate transport by responding to pH changes and ion binding. Emulating this logic, chitosan is introduced into cylindrical nanochannels of multiporous polycarbonate ion-track-etched membranes via covalent immobilization or electrostatic adsorption. Chitosan is confined within the nanochannels, leaving outer-surface liquid-solid interactions unchanged. The channels remain open when dry, while upon hydration the polymer swells and modulates water and ion transport. Chitosan shifts the membrane from microfiltration to ultrafiltration via swelling-driven steric hindrance. Consistent with reduced water flux, the flux of tris(2,2'-bipyridine)ruthenium(II) (Ru(bpy)(3) (2) (+)) decreases by approximately 75% and approximately 92% at pH 7.5 and pH 2.5, respectively, compared to pristine nanochannels. The stronger limitation at pH 2.5 arises from combined steric hindrance and electrostatic exclusion by protonated chitosan. Salts provide an independent stimulus: at neutral pH, they promote electrostatic screening, polymer-chain reorganization, and chitosan deswelling, thereby increasing water flux, whereas under acidic conditions, the addition of KCl or CaCl(2) mainly affects cation transport through electrostatic screening, while chitosan remains highly swollen. This biomimetic platform enables pH- and ion-triggered, bidirectional control of both water flux and cation transport under different pH and ionic conditions and thereby advances nanoporous materials for smart separations, selective ion handling, and adaptive flow regulation. CI - (c) 2026 Wiley-VCH GmbH. FAU - Varol, H Samet AU - Varol HS AUID- ORCID: 0000-0001-8245-2243 AD - Department of Pharmaceutical Science, DISFARM, University of Milan, Milan, Italy. AD - Department of Chemistry "Giacomo Ciamician", University of Bologna, Bologna, Italy. FAU - Di Lisa, Luana AU - Di Lisa L AUID- ORCID: 0009-0005-0304-1478 AD - Department of Chemistry "Giacomo Ciamician", University of Bologna, Bologna, Italy. FAU - Craveri, Lorenzo AU - Craveri L AUID- ORCID: 0009-0004-5381-745X AD - Department of Environment, Land and Infrastructure Engineering, Politecnico di Torino, Torino, Italy. FAU - Pollicino, Antonino AU - Pollicino A AUID- ORCID: 0000-0001-6814-9977 AD - Department of Civil Engineering and Architecture, University of Catania, Catania, Italy. FAU - Gulino, Antonino AU - Gulino A AD - Department of Chemical Sciences, INSTM UdR of Catania, University of Catania, Catania, Italy. FAU - Genovese, Damiano AU - Genovese D AUID- ORCID: 0000-0002-4389-7247 AD - Department of Chemistry "Giacomo Ciamician", University of Bologna, Bologna, Italy. FAU - Tiraferri, Alberto AU - Tiraferri A AUID- ORCID: 0000-0001-9859-1328 AD - Department of Environment, Land and Infrastructure Engineering, Politecnico di Torino, Torino, Italy. FAU - Liguori, Anna AU - Liguori A AUID- ORCID: 0000-0002-0504-3654 AD - Department of Chemistry "Giacomo Ciamician", University of Bologna, Bologna, Italy. FAU - Focarete, Maria Letizia AU - Focarete ML AUID- ORCID: 0000-0002-0458-7836 AD - Department of Chemistry "Giacomo Ciamician", University of Bologna, Bologna, Italy. AD - Health Sciences and Technologies (HST) CIRI, University of Bologna, Ozzano Emilia, Bologna, Italy. LA - eng GR - Alexander von Humboldt Foundation/ GR - ECS00000022/MUR-PNRR project SAMOTHRACE/ GR - J33C22001330009/European Union - NextGenerationEU/ PT - Journal Article DEP - 20261028 PL - Germany TA - Small JT - Small (Weinheim an der Bergstrasse, Germany) JID - 101235338 SB - IM OTO - NOTNLM OT - chitosan OT - ion transport OT - nanochannels OT - nanofiltration OT - nanofluidics OT - stimuli-responsive membranes OT - track-etched membranes EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 02:53 PHST- 2026/09/22 00:00 [revised] PHST- 2025/12/18 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:53 [entrez] AID - e76447 [pii] AID - 10.1002/smll.76447 [doi] PST - aheadofprint SO - Small. 2026 Oct 28:e76447. doi: 10.1002/smll.76447. PMID- 42952277 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1479-9731 (Electronic) IS - 1479-9723 (Linking) VI - 23 DP - 2026 Jan-Dec TI - Telehealth for healthcare delivery in adults with bronchiectasis: A systematic review. PG - 14799731261501160 LID - 10.1177/14799731261501160 [doi] AB - BackgroundThis systematic review examined the feasibility and clinical effectiveness of telehealth in adults with bronchiectasis.MethodsFive electronic databases were searched for telehealth interventions in adults with bronchiectasis. Feasibility and clinical effects data were extracted. Best evidence synthesis was reported as effect size (d), 95% confidence interval (CI).ResultsNine studies were included (n=156 participants). Interventions were pulmonary rehabilitation (n=4 studies), physical activity promotion (n=1 study), airway clearance therapy (n=3 studies), and multidisciplinary treatment (n=1 study). Studies reported high adherence to interventions (73-100%), high patient satisfaction (81-100%), and minor adverse events with selected interventions. Telehealth pulmonary rehabilitation (4 studies; 21 participants) demonstrated improvements in health-related quality of life (HRQOL) with small to large effect sizes at end-intervention (Chronic Respiratory Questionnaire domains: d range 0.23 to 1.78) but mixed effects on psychological symptoms (d range -1.12 to 0.04). Telehealth airway clearance therapy improved HRQOL (St Georges Respiratory Questionnaire total score: mean difference -5.8 [95% CI -10.8 to -0.9]).ConclusionsTelehealth pulmonary rehabilitation and airway clearance interventions are low risk, feasible and may be clinically effective in this population. Small sample sizes underscore the need for larger trials. Little is known about other telehealth-delivered interventions in bronchiectasis. FAU - Hearn, Kirsty AU - Hearn K AUID- ORCID: 0009-0009-0422-0733 AD - Department of Physiotherapy, Monash University, Melbourne, VIC, Australia. RINGGOLD: 2541 AD - Department of Physiotherapy, Melbourne, VIC, Australia. RINGGOLD: 1890 FAU - Holland, Anne E AU - Holland AE AD - School of Translational Medicine, Monash University, Melbourne, VIC, Australia. RINGGOLD: 2541 AD - Institute for Breathing and Sleep, Austin Health, Melbourne, VIC, Australia. RINGGOLD: 568983 FAU - Lee, Annemarie L AU - Lee AL AUID- ORCID: 0000-0002-8631-0135 AD - Department of Physiotherapy, Monash University, Melbourne, VIC, Australia. RINGGOLD: 2541 AD - Institute for Breathing and Sleep, Austin Health, Melbourne, VIC, Australia. RINGGOLD: 568983 LA - eng PT - Journal Article PT - Systematic Review DEP - 20261028 PL - England TA - Chron Respir Dis JT - Chronic respiratory disease JID - 101197408 SB - IM MH - Humans MH - *Bronchiectasis/therapy/rehabilitation MH - Quality of Life MH - *Telemedicine MH - Patient Satisfaction MH - *Respiratory Therapy/methods MH - Adult MH - Digital Health OTO - NOTNLM OT - bronchiectasis OT - physiotherapy OT - telehealth COIS- Declaration of conflicting interestsThe authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 CRDT- 2026/10/29 02:53 PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:53 [entrez] AID - 10.1177/14799731261501160 [doi] PST - ppublish SO - Chron Respir Dis. 2026 Jan-Dec;23:14799731261501160. doi: 10.1177/14799731261501160. Epub 2026 Oct 28. PMID- 42952273 OWN - NLM STAT- Publisher LR - 20261029 IS - 1469-7793 (Electronic) IS - 0022-3751 (Linking) DP - 2026 Oct 28 TI - Prolonged rod photoresponse and enhanced cone sensitivity in the transgenic mouse retina lacking arrestin and guanylate cyclase activating proteins. LID - 10.1113/JP291252 [doi] AB - Light activates visual pigments and downstream G protein signalling cascades in rod and cone photoreceptor cells. Timely response inactivation requires visual pigment inactivation by arrestin and restoration of cellular cGMP by guanylate cyclase activating proteins (GCAPs). Using GCAP-deficient mice, we previously showed that apo-opsin produces photoresponse-like signals. To investigate the inactivation of apo-opsin by arrestin, we generated GCAP-deficient mice lacking arrestin (Gcaps(-/-)Arr1(-/-)). Using ex vivo electroretinography, we found that the dim flash responses of Gcaps(-/-)Arr1(-/-) rods were approximately 100-fold slower than those of control Gcaps(-/-) rods. As a result, even brief dim light exposure during sample preparation resulted in prolonged rod saturation, so that only cone responses were initially observed in Gcaps(-/-)Arr1(-/-) retinas. In contrast, rod responses were observed immediately when Gcaps(-/-)Arr1(-/-) retinas were dissected under infrared light to avoid rod photoactivation. Microspectrophotometry showed that the rate of production and decay of the active rhodopsin photoproduct, Meta II, was comparable between Gcaps(-/-) and Gcaps(-/-)Arr1(-/-) rods. However, the rate of photoproduct Meta III decay was 3-fold slower in Gcaps(-/-)Arr1(-/-) rods, supporting a role for arrestin in accelerating the decay of photoactivated rhodopsin. To evaluate the effect of rod saturation on cone responses, we compared photopic cone responses from wild-type and Gnat1(-/-) retinas lacking rod signalling. Cone photoresponses were larger and more sensitive in wild-type retinas, suggesting the potentiation of cone function by the saturation of rod phototransduction. Together, our results demonstrate that the very slow deactivation of rod responses in Gcaps(-/-)Arr1(-/-) retinas might result in selective and potentiated cone signalling in darkness. KEY POINTS: Arrestin and guanylate cyclase activating proteins (GCAPs) play an important role in inactivation of the phototransduction cascade and termination of the light response in rod photoreceptors. The deletion of arrestin and GCAPs results in approximately 100-fold slower dim flash responses in rods so that even brief dim light exposure produces saturation of the rods for tens of minutes. The decay of the rhodopsin photoactivated intermediate Meta III was slowed down 3-fold by the deletion of arrestin in Gcaps(-/-) rods, supporting a role for arrestin in accelerating the decay of photoactivated rhodopsin. Cone photoresponses were smaller and less sensitive in retinas lacking rod signalling, suggesting potentiation of cone function by the saturation of rod phototransduction. Together, these results demonstrate that the very slow deactivation of rod responses in arrestin- and GCAP-deficient retinas can result in selective and potentiated cone signalling in darkness. CI - (c) 2026 The Author(s). The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society. FAU - Sato, Shinya AU - Sato S AD - Department of Ophthalmology and Visual Sciences, Gavin Herbert Eye Institute -Brunson Center for Translational Vision Research, University of California, Irvine, California, USA. FAU - Frederiksen, Rikard AU - Frederiksen R AD - Jules Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, California, USA. FAU - Sampath, Alapakkam P AU - Sampath AP AUID- ORCID: 0000-0002-0785-9577 AD - Jules Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, California, USA. FAU - Kefalov, Vladimir J AU - Kefalov VJ AUID- ORCID: 0000-0002-1659-008X AD - Department of Ophthalmology and Visual Sciences, Gavin Herbert Eye Institute -Brunson Center for Translational Vision Research, University of California, Irvine, California, USA. AD - Department of Physiology and Biophysics, University of California, Irvine, California, USA. LA - eng GR - Research to Prevent Blindness/ GR - P30 EY00331/Jules Stein Eye Institute Core NEI/ GR - Gavin Herbert Eye Institute at the University of California, Irvine,/ GR - EY035884/EY/NEI NIH HHS/United States GR - EY029817/EY/NEI NIH HHS/United States GR - EY034070/EY/NEI NIH HHS/United States GR - EY00331/EY/NEI NIH HHS/United States PT - Journal Article DEP - 20261028 PL - England TA - J Physiol JT - The Journal of physiology JID - 0266262 SB - IM OTO - NOTNLM OT - arrestin OT - calcium feedback OT - photoreceptor cell OT - phototransduction OT - rod-cone coupling EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 02:53 PHST- 2026/02/23 00:00 [received] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:53 [entrez] AID - 10.1113/JP291252 [doi] PST - aheadofprint SO - J Physiol. 2026 Oct 28. doi: 10.1113/JP291252. PMID- 42952269 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1557-0681 (Electronic) IS - 1478-2189 (Linking) VI - 24 IP - 4 DP - 2026 Dec TI - Flipping Patients' Understanding of Pain-Flipped Learning Pain Education Model in Physical Therapy. LID - e70295 [pii] LID - 10.1002/msc.70295 [doi] AB - BACKGROUND: Chronic pain affects more than 1 in 5 adults in the United States. Our understanding of pain has improved, particularly regarding psychosocial factors. Compelling evidence supports pain neuroscience education for chronic pain, but the best methods for implementation in clinical practice remain unclear. OBJECTIVE: The aim was to evaluate the impact of a flipped learning pain education model in physical therapy on the function of patients with chronic pain. In addition, the purpose was to describe the predicted and actual number of physical therapy visits in this population. METHODS: A retrospective, uncontrolled single-therapist chart review was conducted. Adults diagnosed with chronic pain who received physical therapy using the flipped learning pain education model and had pre- and post-rehabilitation Patient-Specific Functional Scale (PSFS) scores were included. The main outcome was change in the PSFS. The number of predicted visits was derived from the Focus on Therapeutic Outcomes tool. RESULTS: A total of 28 individuals (68% women) were identified. PSFS scores significantly increased from a median of 2.0 (interquartile range, IQR 3.2) to 8.6 (IQR 2.9) (p < 0.001). At baseline, the median predicted number of visits was 12.0 (IQR 4.0), while the median actual visits were 5.0 (IQR 1.8). A total of 24 patients (85.7%) achieved or exceeded the minimal clinically important difference. CONCLUSIONS: PSFS scores were higher at the final recorded visit in this selected complete-case cohort receiving physical therapy using a flipped learning model. The findings should be interpreted with caution due to the exploratory nature of the study. CI - (c) 2026 John Wiley & Sons Ltd. All rights reserved, including rights for text and data mining and training of artificial intelligence technologies or similar technologies. FAU - Prasad, Sharna AU - Prasad S AD - Samaritan Physical Rehabilitation, Lebanon Community Hospital, Lebanon, Oregon, USA. FAU - Bishop, Kelly AU - Bishop K AD - Department of Physical Therapy Education, College of Health Sciences-Northwest, Western University of Health Sciences, Lebanon, Oregon, USA. FAU - Cabalum, Emma Ruth AU - Cabalum ER AD - Department of Physical Therapy Education, College of Health Sciences-Northwest, Western University of Health Sciences, Lebanon, Oregon, USA. FAU - Nguyen, Jennifer AU - Nguyen J AUID- ORCID: 0009-0003-6371-5652 AD - Department of Physical Therapy Education, College of Health Sciences-Northwest, Western University of Health Sciences, Lebanon, Oregon, USA. FAU - Prescott, Emily AU - Prescott E AD - Department of Physical Therapy Education, College of Health Sciences-Northwest, Western University of Health Sciences, Lebanon, Oregon, USA. FAU - Sowers, Dillon AU - Sowers D AD - Department of Physical Therapy Education, College of Health Sciences-Northwest, Western University of Health Sciences, Lebanon, Oregon, USA. FAU - Walker, Amy AU - Walker A AD - Department of Physical Therapy Education, College of Health Sciences-Northwest, Western University of Health Sciences, Lebanon, Oregon, USA. FAU - Raisanen, Anu M AU - Raisanen AM AUID- ORCID: 0000-0003-3056-8169 AD - Office of the Provost and Academic Affairs, Elon University, Elon, North Carolina, USA. LA - eng PT - Journal Article PL - England TA - Musculoskeletal Care JT - Musculoskeletal care JID - 101181344 SB - IM MH - Humans MH - Female MH - *Physical Therapy Modalities MH - Male MH - *Chronic Pain/rehabilitation/therapy MH - Middle Aged MH - Retrospective Studies MH - *Patient Education as Topic/methods MH - Adult MH - Aged OTO - NOTNLM OT - chronic pain OT - pain management OT - rehabilitation EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 CRDT- 2026/10/29 02:52 PHST- 2026/09/14 00:00 [revised] PHST- 2025/06/23 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:52 [entrez] AID - e70295 [pii] AID - 10.1002/msc.70295 [doi] PST - ppublish SO - Musculoskeletal Care. 2026 Dec;24(4):e70295. doi: 10.1002/msc.70295. PMID- 42952264 OWN - NLM STAT- Publisher LR - 20261029 IS - 1520-5711 (Electronic) IS - 1054-3406 (Linking) DP - 2026 Oct 28 TI - Estimand-based design strategies for cardiovascular recurrent event trials of combination therapies. PG - 1-40 LID - 10.1080/10543406.2026.2747684 [doi] AB - Cardiovascular trials increasingly evaluate combination therapies in settings where patients may experience recurrent events such as myocardial infarction, heart failure hospitalization, or stroke. Traditional single-event analyses fail to capture the cumulative disease burden and temporal dynamics, motivating the integration of recurrent event methods within an estimand framework. This manuscript reviews estimand strategies for recurrent event analyses in cardiovascular trials of combination therapies, emphasizing alignment between clinical questions, intercurrent events, and causal interpretation. We compare commonly used models, including the Andersen-Gill, Prentice-Williams-Peterson, and Negative Binomial approaches, highlighting their suitability for different estimands. We distinguish each model's conditional or marginal estimand, its identifiability assumptions, and the difference between sensitivity analyses that vary assumptions under the same estimand and supplementary analyses that target alternative estimands with different intercurrent event strategies. The impact of intercurrent events such as treatment discontinuation, rescue therapy, and mortality on estimand interpretation is explored. Novel methodological considerations specific to combination-therapy trials, including component-specific intercurrent events (ICE), drug-drug interaction estimands, mixed intercurrent event (ICE) strategies, and principal-stratum estimands conditional on dual adherence, are formally defined and discussed with work examples. We further discuss how the choice of estimand influences causal inference and model selection. Finally, we provide a regulatory perspective on estimand-based approaches in cardiovascular trials. This manuscript serves as a methodological guide for designing and analyzing cardiovascular trials with recurrent events for combination therapies to ensure clarity of clinical interpretation, robustness of causal conclusions, and regulatory alignment. FAU - Mukherjee, Ayon AU - Mukherjee A AUID- ORCID: 0000-0002-9655-2605 AD - Population Health Sciences Institute, Newcastle University, Newcastle upon Tyne, UK. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - J Biopharm Stat JT - Journal of biopharmaceutical statistics JID - 9200436 SB - IM OTO - NOTNLM OT - Cardiovascular recurrent events OT - causal inference OT - combination therapy OT - drug-drug interaction estimand OT - mixed ICE strategy OT - regulatory alignment EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 02:44 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:44 [entrez] AID - 10.1080/10543406.2026.2747684 [doi] PST - aheadofprint SO - J Biopharm Stat. 2026 Oct 28:1-40. doi: 10.1080/10543406.2026.2747684. PMID- 42952250 OWN - NLM STAT- Publisher LR - 20261029 IS - 1613-6829 (Electronic) IS - 1613-6810 (Linking) DP - 2026 Oct 28 TI - Designable Nanozymes for Site-Selective Amide/Ester Hydrolysis. LID - e76522 [pii] LID - 10.1002/smll.76522 [doi] AB - Site selectivity in catalysis requires the catalyst to recognize not only the targeted reactive group on the substrate but also its structural context. Such selectivity is vital to many biological catalytic reactions, including those found in the posttranslational modification of proteins. Although site-selective catalysts would enable precise transformations of molecules, polymers, and materials, no general strategy exists for the rational design of site-selective catalysts. We here report nanozymes with tunable substrate-binding sites and strategically positioned catalytic groups. A zinc cofactor, together with proximal acidic and basic groups, activates water for nucleophilic attack on the bound ester or amide. The size/shape of the active site and the position of the catalytic groups are tuned rationally through molecular imprinting, using modularly synthesized acylthiourea templates. These nanozymes enable programmable site-selective hydrolysis of esters and amides, independent of the intrinsic reactivities of the competing functional groups. CI - (c) 2026 The Author(s). Small published by Wiley-VCH GmbH. FAU - Singh, Shashank AU - Singh S AUID- ORCID: 0000-0002-7908-4272 AD - Department of Chemistry, Iowa State University, Ames, Iowa, USA. FAU - Barik, Rasmita AU - Barik R AD - Department of Chemistry, Iowa State University, Ames, Iowa, USA. FAU - Zhao, Yan AU - Zhao Y AUID- ORCID: 0000-0003-1215-2565 AD - Department of Chemistry, Iowa State University, Ames, Iowa, USA. LA - eng GR - GM/NIGMS NIH HHS/United States GR - R35GM156461/NH/NIH HHS/United States PT - Journal Article DEP - 20261028 PL - Germany TA - Small JT - Small (Weinheim an der Bergstrasse, Germany) JID - 101235338 SB - IM OTO - NOTNLM OT - active site OT - artificial enzyme OT - cooperative catalysis OT - hydrolysis OT - molecular imprinting OT - site-selectivity EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 02:43 PHST- 2026/09/30 00:00 [revised] PHST- 2026/03/12 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:43 [entrez] AID - e76522 [pii] AID - 10.1002/smll.76522 [doi] PST - aheadofprint SO - Small. 2026 Oct 28:e76522. doi: 10.1002/smll.76522. PMID- 42952244 OWN - NLM STAT- Publisher LR - 20261029 IS - 1349-7006 (Electronic) IS - 1347-9032 (Print) IS - 1347-9032 (Linking) DP - 2026 Oct 28 TI - NEDD4 Promotes Hepatocellular Carcinoma Progression Through Ubiquitin-Dependent Degradation of EPHB6. LID - 10.1111/cas.70573 [doi] AB - Neural precursor cell expressed developmentally down-regulated 4 (NEDD4) has been implicated in the progression of various human malignancies, including hepatocellular carcinoma (HCC). However, the relationship between NEDD4's ubiquitin ligase activity and the motility of HCC cells remains unclear. We investigated the significance of the NEDD4/EPHB6 (erythropoietin-producing hepatocyte B6) axis in driving epithelial-mesenchymal transition (EMT) progression in HCC. In this study, we investigated the role of the HECT-type E3 ubiquitin ligase NEDD4 in HCC progression and identified its downstream substrate through integrated bioinformatic screening, protein-level validation, and NEDD4 immunoprecipitation coupled with mass spectrometry. The regulatory relationship between NEDD4 and EPHB6 was examined using ubiquitination assays, protein stability analysis, and functional rescue experiments. Potential downstream signaling changes associated with the NEDD4-EPHB6 axis were further examined through pathway analysis and molecular validation. NEDD4 silencing suppressed, whereas its overexpression enhanced HCC cell invasion and migration. Mechanistically, NEDD4 functioned as an E3 ubiquitin ligase that promoted Lys48-linked polyubiquitination and proteasomal degradation of erythropoietin-producing hepatocyte B6 (EPHB6), resulting in reduced EPHB6 protein abundance without altering EPHB6 mRNA levels. Disruption of the NEDD4-EPHB6 axis attenuated EMT, which was further validated in a xenograft mouse model. Moreover, NEDD4 protein expression was inversely correlated with EPHB6 levels in clinical HCC tissues. In summary, our study identifies the NEDD4-EPHB6 axis as a previously unrecognized regulator of EMT-associated malignant progression in HCC and demonstrates its association with activation of TGF-beta/SMAD2/3 signaling. This study provides new mechanistic insight into how post-translational regulation of membrane-associated proteins controls HCC progression. CI - (c) 2026 The Author(s). Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. FAU - Zhang, Guowei AU - Zhang G AD - Institute of Digestive Diseases, Xuzhou Medical University, Xuzhou, China. AD - General Surgery Department, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. AD - Department of General Surgery, Xuzhou Central Hospital, Southeast University, Xuzhou, China. FAU - Ji, Mengxuan AU - Ji M AUID- ORCID: 0009-0005-3800-7974 AD - Institute of Digestive Diseases, Xuzhou Medical University, Xuzhou, China. AD - General Surgery Department, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. FAU - Liu, Zhiyi AU - Liu Z AD - Institute of Digestive Diseases, Xuzhou Medical University, Xuzhou, China. AD - General Surgery Department, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. FAU - Xu, Jiawei AU - Xu J AD - Institute of Digestive Diseases, Xuzhou Medical University, Xuzhou, China. AD - General Surgery Department, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. FAU - Hu, Qinghe AU - Hu Q AD - Institute of Digestive Diseases, Xuzhou Medical University, Xuzhou, China. AD - General Surgery Department, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. FAU - Zhang, Xue AU - Zhang X AD - Institute of Digestive Diseases, Xuzhou Medical University, Xuzhou, China. AD - General Surgery Department, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. FAU - Qiu, Jie AU - Qiu J AD - Institute of Digestive Diseases, Xuzhou Medical University, Xuzhou, China. AD - General Surgery Department, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. FAU - Wu, Kai AU - Wu K AD - Institute of Digestive Diseases, Xuzhou Medical University, Xuzhou, China. AD - General Surgery Department, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. FAU - Zhang, Bin AU - Zhang B AD - Institute of Digestive Diseases, Xuzhou Medical University, Xuzhou, China. AD - General Surgery Department, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. FAU - He, Yi AU - He Y AD - Experimental Teaching Center for Basic Surgery, the First Clinical Medical College, Xuzhou Medical University, Xuzhou, China. FAU - Shi, Hengliang AU - Shi H AUID- ORCID: 0009-0001-1969-4036 AD - Institute of Digestive Diseases, Xuzhou Medical University, Xuzhou, China. AD - General Surgery Department, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. AD - Jiangsu Key Laboratory of Organ Transplantation and Transplant Immunology, Xuzhou, China. AD - Center for Sci-Tech Innovation and Public Service, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. LA - eng GR - BF2025632/Frontier Technology Research and Development Program of Jiangsu (Healthcare Field)/ GR - 82573050/National Natural Science Foundation of China/ GR - PYJH2025305/Advanced Program of The Affiliated Hospital of Xuzhou Medical University/ GR - KYCX25_3317/Jiangsu Postgraduate Research Innovation Program/ GR - 2025TD03/New Round of Xuzhou "Pengcheng Talent Program"-High-level Healthcare Talent Recruitment and Development Project/ GR - GSPJS202401/Construction Project of High Level Hospital of Jiangsu Province/ GR - GSPJS202502/Construction Project of High Level Hospital of Jiangsu Province/ GR - PT0121/Jiangsu Key Laboratory of Organ Transplantation and Transplant Immunology/ PT - Journal Article DEP - 20261028 PL - England TA - Cancer Sci JT - Cancer science JID - 101168776 SB - IM PMC - PMC13717255 OTO - NOTNLM OT - EMT OT - EPHB6 OT - HCC OT - NEDD4 OT - ubiquitination COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 PMCR- 2026/10/28 CRDT- 2026/10/29 02:43 PHST- 2026/10/05 00:00 [revised] PHST- 2026/04/01 00:00 [received] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:43 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - CAS70573 [pii] AID - 10.1111/cas.70573 [doi] PST - aheadofprint SO - Cancer Sci. 2026 Oct 28:10.1111/cas.70573. doi: 10.1111/cas.70573. PMID- 42952241 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1557-0681 (Electronic) IS - 1478-2189 (Print) IS - 1478-2189 (Linking) VI - 24 IP - 4 DP - 2026 Dec TI - Accuracy, Quality and Readability of Knee Osteoarthritis Information on Social Media (Instagram e TikTok) and Search Platforms (Google e YouTube): A Cross-Sectional Study. LID - e70267 [pii] LID - 10.1002/msc.70267 [doi] AB - BACKGROUND: Knee osteoarthritis (KOA) is a chronic condition with a high global prevalence, often resulting in functional decline. Faced with this, many people turn to the internet to find information about the disease and available treatments. Besides the amount of content available online, it does not ensure its accuracy, quality and readability. This study aims to evaluate the accuracy, quality and readability of KOA-related information available on Instagram, TikTok, Google and YouTube in Brazilian Portuguese. METHODS: Google and YouTube searches were performed using 'Tratamento de osteoartrite de joelho', 'Tratamento de artrose no joelho' and 'Tratamento de artrite no joelho'. For each term, the first 20 results were selected. Searches on Instagram and TikTok were conducted using the hashtags: #artrosenojoelho, #artrosedojoelho, and #artrosedejoelho, selecting the first 100 posts for each term. All the research was done on June 19, 2024. Content accuracy was assessed against a reference resource on knee osteoarthritis, information quality was assessed using DISCERN, and the readability of Google content was evaluated using the Flesch-Kincaid Grade Level scale. RESULTS: We found that the information about medical management was the most classified as inaccurate. The overall DISCERN score indicated low quality (2.3 +/- 0.7) and 78% of Google texts required at least 14 years of schooling to read. CONCLUSION: As digital platforms have become a widely used source of health information, our findings raise concerns about the potential for misinformation to delay effective treatment and perpetuate low-value care. CI - (c) 2026 The Author(s). Musculoskeletal Care published by John Wiley & Sons Ltd. FAU - Castro, Rayane Quintao AU - Castro RQ AUID- ORCID: 0000-0002-8452-2740 AD - Programa de pos-graduacao em Saude, Faculdade de Medicina, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil. FAU - Ferreira de Oliveira, Giovanna AU - Ferreira de Oliveira G AUID- ORCID: 0009-0006-2119-0407 AD - Programa de pos-graduacao em Ciencias da Reabilitacao e Desempenho Fisico Funcional, Faculdade de Fisioterapia, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil. FAU - Marques, Marcella Nogueira AU - Marques MN AUID- ORCID: 0009-0000-1216-9098 AD - Programa de pos-graduacao em Saude, Faculdade de Medicina, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil. FAU - Silva, Victoria Beatriz AU - Silva VB AUID- ORCID: 0009-0005-4868-3378 AD - Faculdade de Fisioterapia, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil. FAU - Fonseca, Diogo Simoes AU - Fonseca DS AUID- ORCID: 0000-0002-3904-839X AD - Programa de pos-graduacao em Ciencias da Reabilitacao e Desempenho Fisico Funcional, Faculdade de Fisioterapia, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil. AD - Faculdade de Fisioterapia, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil. FAU - Malaguti, Carla AU - Malaguti C AUID- ORCID: 0000-0002-6619-136X AD - Programa de pos-graduacao em Saude, Faculdade de Medicina, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil. AD - Programa de pos-graduacao em Ciencias da Reabilitacao e Desempenho Fisico Funcional, Faculdade de Fisioterapia, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil. AD - Faculdade de Fisioterapia, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil. LA - eng GR - 001/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/ PT - Journal Article PL - England TA - Musculoskeletal Care JT - Musculoskeletal care JID - 101181344 SB - IM MH - *Osteoarthritis, Knee MH - Humans MH - *Social Media/standards MH - Cross-Sectional Studies MH - *Comprehension MH - *Consumer Health Information/standards MH - Brazil MH - Internet MH - Digital Media PMC - PMC13717208 OTO - NOTNLM OT - accuracy OT - knee osteoarthritis OT - quality OT - readability COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/29 CRDT- 2026/10/29 02:42 PHST- 2026/08/15 00:00 [revised] PHST- 2025/11/26 00:00 [received] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:42 [entrez] PHST- 2026/10/29 00:00 [pmc-release] AID - e70267 [pii] AID - MSC70267 [pii] AID - 10.1002/msc.70267 [doi] PST - ppublish SO - Musculoskeletal Care. 2026 Dec;24(4):e70267. doi: 10.1002/msc.70267. PMID- 42952233 OWN - NLM STAT- Publisher LR - 20261029 IS - 1613-6829 (Electronic) IS - 1613-6810 (Linking) DP - 2026 Oct 28 TI - Selective Interfacial Regulation of LiMn(2)O(4) for Flow Electrochemical Lithium Extraction. LID - e76531 [pii] LID - 10.1002/smll.76531 [doi] AB - Gas-produced water (GPW) is increasingly recognized as an important low-quality aqueous lithium resource. Electrochemical lithium extraction using LiMn(2)O(4) (LMO) offers a promising strategy for lithium extraction from aqueous resources. However, its application to GPW is hindered by competing-ion co-intercalation and Mn dissolution, which compromise extraction selectivity and stability. Inspired by selective ion transport in biological ion pumps, a Li(0.33)La(0.56)TiO(3) (LLTO)-engineered LMO interface is developed to integrate Li(+)-sieving nanochannels with interfacial stabilization. Experimental and mechanistic analyses demonstrate that the LLTO-engineered interface promotes local Li(+) enrichment, while its Li(+)-sieving nanochannels restrict competing-cation access, thereby accelerating selective Li(+) transport and optimizing Li(+) storage. Concurrently, the LLTO suppresses Mn dissolution, thereby preserving interfacial stability during repeated Li(+) extraction and release. Furthermore, the cyclic flow 3LLTO-LMO//3LLTO-lambda-MnO(2) system delivers 16.6 mg.g(-1) lithium release capacity after 10 cycles and achieves an 87.5% Li(+) recovery rate while retaining 60.7% Li(+) purity from simulated GPW after 20 cycles, and further demonstrates applicability to real GPW. This work establishes a Li(+)-selective interfacial regulation strategy for efficient and stable lithium extraction and provides a material-to-process framework for sustainable lithium extraction from low-quality aqueous lithium resources. CI - (c) 2026 Wiley-VCH GmbH. FAU - Gu, Jun AU - Gu J AD - College of Science, State Key Laboratory of Heavy Oil Processing, Key Lab of Oil & Gas Optical Detection Technology, China University of Petroleum-Beijing, Beijing, People's Republic of China. FAU - Chao, Yanhong AU - Chao Y AUID- ORCID: 0000-0001-7075-6944 AD - College of Science, State Key Laboratory of Heavy Oil Processing, Key Lab of Oil & Gas Optical Detection Technology, China University of Petroleum-Beijing, Beijing, People's Republic of China. FAU - Tang, Minmeng AU - Tang M AUID- ORCID: 0000-0002-2848-9712 AD - College of Chemical Engineering and Environment, State Key Laboratory of Heavy Oil Processing, China University of Petroleum-Beijing, Beijing, People's Republic of China. FAU - Zhang, Li AU - Zhang L AD - College of Science, State Key Laboratory of Heavy Oil Processing, Key Lab of Oil & Gas Optical Detection Technology, China University of Petroleum-Beijing, Beijing, People's Republic of China. FAU - Chen, Linlin AU - Chen L AUID- ORCID: 0000-0002-5370-5624 AD - School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, People's Republic of China. FAU - He, Muyao AU - He M AD - College of Science, State Key Laboratory of Heavy Oil Processing, Key Lab of Oil & Gas Optical Detection Technology, China University of Petroleum-Beijing, Beijing, People's Republic of China. FAU - Zhu, Wenshuai AU - Zhu W AUID- ORCID: 0000-0002-7782-5794 AD - College of Chemical Engineering and Environment, State Key Laboratory of Heavy Oil Processing, China University of Petroleum-Beijing, Beijing, People's Republic of China. AD - Shandong Key Laboratory of Green Electricity & Hydrogen Science and Technology, Shandong Institute of Petroleum and Chemical Technology, Dongying, People's Republic of China. FAU - Liu, Zhichang AU - Liu Z AD - College of Chemical Engineering and Environment, State Key Laboratory of Heavy Oil Processing, China University of Petroleum-Beijing, Beijing, People's Republic of China. FAU - Xu, Chunming AU - Xu C AD - College of Chemical Engineering and Environment, State Key Laboratory of Heavy Oil Processing, China University of Petroleum-Beijing, Beijing, People's Republic of China. LA - eng GR - U25D9013/National Natural Science Foundation of China/ GR - 22494714/National Natural Science Foundation of China/ GR - 22278426/National Natural Science Foundation of China/ GR - 22425808/China National Funds for Distinguished Young Scientists/ GR - CNIF20240103/Carbon Neutrality Research Institute Fund/ GR - HSK202506/Open Fund Project of State Key Laboratory of Synthetic Biology/ GR - 2462026YJRC015/Science Foundation of China University of Petroleum, Beijing/ GR - 2462025BJRC002/Science Foundation of China University of Petroleum, Beijing/ GR - Independent Research Project of the State Key Laboratory of Heavy Oil Processing/ PT - Journal Article DEP - 20261028 PL - Germany TA - Small JT - Small (Weinheim an der Bergstrasse, Germany) JID - 101235338 SB - IM OTO - NOTNLM OT - LiMn2O4 OT - enhancement mechanism OT - flow system OT - low-quality gas-produced water OT - selective interface EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 02:33 PHST- 2026/10/13 00:00 [revised] PHST- 2026/08/27 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:33 [entrez] AID - e76531 [pii] AID - 10.1002/smll.76531 [doi] PST - aheadofprint SO - Small. 2026 Oct 28:e76531. doi: 10.1002/smll.76531. PMID- 42952230 OWN - NLM STAT- Publisher LR - 20261029 IS - 1521-3773 (Electronic) IS - 1433-7851 (Linking) DP - 2026 Oct 28 TI - Automated Solid-Phase Flow Synthesis for Scaffold Diversification of a High-Throughput Screening Hit. LID - e1500194 [pii] LID - 10.1002/anie.1500194 [doi] AB - High-throughput screening (HTS) enables rapid hit identification, yet subsequent analogue synthesis and structure-activity relationship exploration remain constrained by labor-intensive multistep workflows. Here, we describe an automated solid-phase flow synthesis (SPS-flow) platform for diversity-oriented analogue generation of an HTS-derived, non-peptidic small-molecule hit scaffold. A benzodiazepine hit against cyclin-dependent kinase 11B (CDK11B) was selected as a proof-of-concept and translated into a modular 10-11-step synthetic sequence through fragment-guided retrosynthetic design. A sterically tuned triazene linker on 2-chlorotrityl resin was developed to tolerate diverse reaction conditions while enabling traceless cleavage and post-synthetic diazonium diversification, and Chemical Recipe File (CRF)-based control coordinated continuous-, circulation-, and stop-flow operations within a unified automated workflow. The platform enabled the synthesis of 42 benzodiazepine analogues in 5%-33% overall yields, with each sequence completed within approximately 45-48 h from a common resin-bound precursor. Within the explored first-generation design space, the library rapidly defined the synthetic accessibility and preliminary structure-activity landscape of the scaffold under SPS-flow conditions, supporting early triage of the series and demonstrating that SPS-flow can be used as a programmable platform for automated multistep diversification and early-stage drug optimization. CI - (c) 2026 Wiley-VCH GmbH. FAU - Yu, Zhenyang AU - Yu Z AUID- ORCID: 0000-0002-5335-3948 AD - Department of Chemistry, National University of Singapore, Singapore, Singapore. AD - National University of Singapore (Chongqing) Research Institute, Chongqing, China. FAU - Zhang, Zhi-Qi AU - Zhang ZQ AD - Department of Chemistry, National University of Singapore, Singapore, Singapore. FAU - Wu, Jiale AU - Wu J AUID- ORCID: 0009-0001-8053-9991 AD - Department of Chemistry, National University of Singapore, Singapore, Singapore. FAU - Zhang, Yanbin AU - Zhang Y AD - Department of Chemistry, National University of Singapore, Singapore, Singapore. FAU - Ang, Hwee Ting AU - Ang HT AD - Department of Chemistry, National University of Singapore, Singapore, Singapore. FAU - Zhou, Feng AU - Zhou F AD - Department of Chemistry, National University of Singapore, Singapore, Singapore. FAU - Ang, Jackie AU - Ang J AD - Experimental Drug Development Centre (EDDC), Agency for Science, Technology and Research (A*STAR), Chromos, Singapore. FAU - Wong, Mei Yee AU - Wong MY AD - Experimental Drug Development Centre (EDDC), Agency for Science, Technology and Research (A*STAR), Chromos, Singapore. FAU - Hai, Ronald Toh Kuan AU - Hai RTK AD - Experimental Drug Development Centre (EDDC), Agency for Science, Technology and Research (A*STAR), Chromos, Singapore. FAU - Mak, Frankie S AU - Mak FS AD - Experimental Drug Development Centre (EDDC), Agency for Science, Technology and Research (A*STAR), Chromos, Singapore. FAU - Wu, Jie AU - Wu J AUID- ORCID: 0000-0002-9865-180X AD - Department of Chemistry, National University of Singapore, Singapore, Singapore. AD - National University of Singapore (Chongqing) Research Institute, Chongqing, China. LA - eng GR - NRF-CRP25-2020RS-0002/National Research Foundation/ GR - A-8003842-00-00/Pfizer/ PT - Journal Article DEP - 20261028 PL - Germany TA - Angew Chem Int Ed Engl JT - Angewandte Chemie (International ed. in English) JID - 0370543 SB - IM OTO - NOTNLM OT - automated synthesis OT - chemical recipe file OT - flow chemistry OT - medicinal chemistry OT - solid-phase synthesis EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 02:33 PHST- 2026/10/19 00:00 [revised] PHST- 2026/07/24 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:33 [entrez] AID - e1500194 [pii] AID - 10.1002/anie.1500194 [doi] PST - aheadofprint SO - Angew Chem Int Ed Engl. 2026 Oct 28:e1500194. doi: 10.1002/anie.1500194. PMID- 42952229 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2045-7634 (Electronic) IS - 2045-7634 (Linking) VI - 15 IP - 11 DP - 2026 Nov TI - Virtual Social Interaction and Psychological Capital in Chemotherapy Patients: A Randomized Controlled Trial. LID - e72419 [pii] LID - 10.1002/cam4.72419 [doi] AB - BACKGROUND: Cancer-related psychological distress remains underaddressed globally. Virtual interventions offer promising alternatives but lack evidence for enhancing Psychological Capital (PsyCap) in chemotherapy patients. OBJECTIVE: This study aimed to evaluate the efficacy of a virtual social interaction intervention on PsyCap in patients undergoing chemotherapy. METHODS: This single-blind randomized controlled trial (RCT) enrolled 76 chemotherapy patients hospitalized in the oncology ward of Imam Sajjad Hospital, Ramsar, Mazandaran, Iran. Participants were selected via convenience sampling and then allocated to intervention or control groups using an odd-even (alternating) assignment method. The intervention group participated in 6 weeks of virtual social interactions, consisting of asynchronous materials (recorded audio, short videos, images, PDF, and PowerPoint files) uploaded weekly to Iranian messaging platforms (Eitaa), as well as live synchronous group sessions conducted on the Skyroom platform with a minimum of 10 participants per session. The control group received standard care. The PsyCap was measured using Luthans' Psychological Capital Questionnaire at both baseline and post-intervention. The Analysis of Covariance (ANCOVA) models (A/B) were adjusted for baseline scores and age. RESULTS: The intervention group showed improvements across all outcomes, with the total PsyCap score increasing by 23.6 points. Components significantly increased: self-efficacy by 5.8 points, resilience by 5.8, hope by 5.5, and optimism by 6.5 (p < 0.0001). ANCOVA models confirmed robust effects after adjusting for baseline scores and age (Delta SMD > 1). CONCLUSIONS: Virtual social interactions significantly enhance PsyCap in chemotherapy patients. However, to confidently generalize these findings and make recommendations, similar studies need to be conducted and replicated in larger, multicenter trials. CI - (c) 2026 The Author(s). Cancer Medicine published by John Wiley & Sons Ltd. FAU - Rafat, Masome AU - Rafat M AUID- ORCID: 0009-0008-7665-4234 AD - Student Research Committee, Nursing Care Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R. Iran. FAU - Pourhabib, Ali AU - Pourhabib A AUID- ORCID: 0000-0002-6355-1980 AD - Nursing Care Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R. Iran. FAU - Mahmoudian, Amaneh AU - Mahmoudian A AUID- ORCID: 0000-0002-0970-8318 AD - Nursing Care Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R. Iran. FAU - Keyhanian, Shahrbanoo AU - Keyhanian S AUID- ORCID: 0000-0003-3810-4131 AD - Faculty of Medicine, Islamic Azad University, Tonekabon Branch, Tonekabon, I.R. Iran. FAU - Fotokian, Zahra AU - Fotokian Z AUID- ORCID: 0000-0002-9274-7413 AD - Nursing Care Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R. Iran. FAU - Kheirkhah, Farzan AU - Kheirkhah F AUID- ORCID: 0000-0003-1420-5480 AD - Social Determinants of Health Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R. Iran. LA - eng PT - Journal Article PT - Randomized Controlled Trial PL - United States TA - Cancer Med JT - Cancer medicine JID - 101595310 RN - 0 (Antineoplastic Agents) SB - IM MH - Humans MH - Female MH - Male MH - *Neoplasms/psychology/drug therapy MH - Middle Aged MH - Single-Blind Method MH - *Social Interaction MH - Adult MH - *Antineoplastic Agents/therapeutic use MH - Iran MH - Surveys and Questionnaires MH - Psychological Distress MH - *Stress, Psychological PMC - PMC13717221 OTO - NOTNLM OT - chemotherapy OT - nursing education OT - psychological capital OT - psychological resilience OT - psycho-oncology OT - randomized controlled trial OT - social media OT - telemedicine COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/28 CRDT- 2026/10/29 02:33 PHST- 2026/09/30 00:00 [revised] PHST- 2025/10/06 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:33 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e72419 [pii] AID - CAM472419 [pii] AID - 10.1002/cam4.72419 [doi] PST - ppublish SO - Cancer Med. 2026 Nov;15(11):e72419. doi: 10.1002/cam4.72419. PMID- 42952215 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1976-9148 (Print) IS - 1976-9148 (Linking) VI - 34 IP - 6 DP - 2026 Nov 1 TI - SD-2102, a Novel PDK1 Inhibitor, Suppresses Lung Cancer Growth by Targeting PDK1/Akt Signaling Pathway. PG - 1413-1423 LID - 10.4062/biomolther.2026.131 [doi] AB - The phosphoinositide-dependent kinase-1 (PDK1)-Akt signaling pathway plays a central role in regulating diverse cellular processes, including cell growth, survival, and metabolism. Dysregulation of this pathway is frequently observed in malignancies and is associated with tumor progression and resistance to therapy. Despite the recognized therapeutic potential of PDK1, potent and selective inhibitors remain limited. In this study, we developed and characterized a novel PDK1 inhibitor, SD-2102, and evaluated its anticancer effects in vitro and in vivo. A novel small-molecule compound, SD-2102, was synthesized and screened for PDK1 inhibitory activity. Binding affinity and interaction sites were examined using biochemical assays to identify the targeted domain. Functional assays, including cell proliferation, cell cycle analysis, and apoptosis detection, were performed in cancer cell lines. The in vivo efficacy of SD-2102 was assessed using a xenograft model. SD-2102 was predicted to interact with the pleckstrin homology domain of PDK1 and reduced PDK1 plasma membrane translocation. This inhibition led to decreased phosphorylation and activation of Akt. In vitro, SD-2102 suppressed cancer cell proliferation by inducing G1 phase arrest and promoting apoptosis. Importantly, SD-2102 induced minimal apoptosis in BEAS-2B non-tumorigenic human bronchial epithelial cells, supporting a preferential apoptotic effect toward lung cancer cells. In vivo, treatment with SD-2102 significantly reduced tumor growth in the xenograft model, consistent with the cellular findings. Together, these findings suggest that SD-2102 suppresses PDK1/Akt signaling while exhibiting preferential anticancer activity in lung cancer cells, supporting its further development as an anticancer agent. FAU - Namgung, Hee AU - Namgung H AD - Department of Health Sciences and Technology, GAIHST, Gachon University, Incheon 21999, Republic of Korea. FAU - Park, Se Yong AU - Park SY AD - College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea. FAU - Darlami, Om AU - Darlami O AD - College of Pharmacy, Gachon University, Incheon 21936, Republic of Korea. FAU - Hwang, Jung Ho AU - Hwang JH AD - College of Pharmacy, Gachon University, Incheon 21936, Republic of Korea. FAU - Kang, Ju-Hee AU - Kang JH AD - College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea. FAU - Lee, Yoonji AU - Lee Y AD - College of Pharmacy, Chung-Ang University, Seoul 06974, Republic of Korea. FAU - Shin, Dongyun AU - Shin D AD - College of Pharmacy, Gachon University, Incheon 21936, Republic of Korea. FAU - Oh, Seung Hyun AU - Oh SH AD - College of Veterinary Medicine, Seoul National University, Seoul 08826, Republic of Korea. LA - eng PT - Journal Article DEP - 20261030 PL - Korea (South) TA - Biomol Ther (Seoul) JT - Biomolecules & therapeutics JID - 101472832 OTO - NOTNLM OT - Anticancer agent OT - Lung cancer OT - PDK1 inhibitor OT - PDK1/Akt pathway EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 CRDT- 2026/10/29 02:33 PHST- 2026/06/09 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:33 [entrez] AID - biomolther.2026.131 [pii] AID - 10.4062/biomolther.2026.131 [doi] PST - ppublish SO - Biomol Ther (Seoul). 2026 Nov 1;34(6):1413-1423. doi: 10.4062/biomolther.2026.131. Epub 2026 Oct 30. PMID- 42952212 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1976-9148 (Print) IS - 1976-9148 (Linking) VI - 34 IP - 6 DP - 2026 Nov 1 TI - Emerging Roles of ETV4 in Tumor Microenvironment Remodeling: Mechanisms and Therapeutic Targeting. PG - 1350-1367 LID - 10.4062/biomolther.2026.180 [doi] AB - ETS variant transcription factor 4 (ETV4), a member of the PEA3 subfamily of ETS transcription factors, has emerged as an oncogenic transcriptional regulator that promotes tumor growth, invasion, metastasis, and therapeutic resistance. Although previous studies have largely focused on its cancer cell-intrinsic functions, accumulating evidence suggests that ETV4 also contributes to remodeling of the tumor microenvironment (TME). ETV4 influences diverse microenvironmental processes, including metabolic adaptation, immune crosstalk, stromal and extracellular matrix remodeling, and cellular responses to hypoxic and mechanical stress. It has also been implicated in extracellular vesicle- and non-coding RNA-mediated communication, further supporting its role in coordinating reciprocal interactions between tumor cells and their surrounding microenvironment. Collectively, these findings position ETV4 as a molecular interface linking oncogenic signaling to TME reprogramming. In this review, we synthesize current evidence on the involvement of ETV4 in metabolic, immune, stromal, extracellular matrix, hypoxic, and intercellular communication networks. We further discuss emerging therapeutic strategies aimed at suppressing ETV4-associated activity through inhibition of upstream RTK-MAPK signaling, disruption of transcriptional cofactor dependencies, modulation of protein stability, and targeting of ETV4-dependent microenvironmental interactions. A more comprehensive understanding of ETV4-mediated tumor-microenvironment crosstalk may uncover context-specific vulnerabilities and provide a rationale for combination therapeutic strategies in ETV4-active malignancies. FAU - Yu, Su Hyeon AU - Yu SH AD - Jeju Research Institute of Pharmaceutical Sciences, College of Pharmacy, Jeju National University, Jeju 63243, Republic of Korea. FAU - Liu, Xiaodong AU - Liu X AD - Jeju Research Institute of Pharmaceutical Sciences, College of Pharmacy, Jeju National University, Jeju 63243, Republic of Korea. FAU - Kang, Jiyoon AU - Kang J AD - Jeju Research Institute of Pharmaceutical Sciences, College of Pharmacy, Jeju National University, Jeju 63243, Republic of Korea. FAU - Kim, Ji Won AU - Kim JW AD - Jeju Research Institute of Pharmaceutical Sciences, College of Pharmacy, Jeju National University, Jeju 63243, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20261030 PL - Korea (South) TA - Biomol Ther (Seoul) JT - Biomolecules & therapeutics JID - 101472832 OTO - NOTNLM OT - Cancer-associated fibroblasts OT - ETS variant transcription factor 4 OT - Immune crosstalk OT - Metabolic reprogramming OT - Therapeutic resistance OT - Tumor microenvironment EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 CRDT- 2026/10/29 02:33 PHST- 2026/07/27 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:33 [entrez] AID - biomolther.2026.180 [pii] AID - 10.4062/biomolther.2026.180 [doi] PST - ppublish SO - Biomol Ther (Seoul). 2026 Nov 1;34(6):1350-1367. doi: 10.4062/biomolther.2026.180. Epub 2026 Oct 30. PMID- 42952208 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261029 LR - 20261029 IS - 1976-9148 (Print) IS - 1976-9148 (Linking) VI - 34 IP - 6 DP - 2026 Nov 1 TI - Beyond Receptor-Proximal Uncoupling: EGFR-PKC Signaling and Arrestin Deubiquitination-Coupled Nuclear Trafficking in GPCR Signaling Regulation. PG - 1283-1298 LID - 10.4062/biomolther.2026.132 [doi] AB - G protein-coupled receptors (GPCRs) constitute the largest family of cell-surface signaling proteins and major therapeutic targets. Their function is critically shaped by desensitization, the attenuation of receptor responsiveness during sustained or repeated stimulation. The canonical model, established in systems such as rhodopsin and the beta(2)-adrenergic receptor, emphasizes GRK-mediated receptor phosphorylation, arrestin recruitment, and steric inhibition of G-protein coupling, resulting in rapid and largely reversible receptor desensitization. Accumulating evidence, however, indicates that GPCR adaptation can extend beyond this receptor-proximal mechanism. Here, I propose a Two-Level Framework that incorporates an EGFR-dependent adaptive program following sustained activation of selected GPCRs. EGFR serves as an upstream integration hub linking two complementary pathways: a PKC-dependent proximal pathway that mediates receptor-level desensitization, and a Src-PDK1-Akt-USP33 pathway proposed to promote arrestin deubiquitination and subsequent nuclear translocation of arrestin-Gbetagamma complexes. This translocation may reduce membrane-associated Gbetagamma availability, thereby contributing to persistent attenuation of receptor signaling, while also influencing nuclear transcriptional activity. The persistent component is revealed by washout-rechallenge paradigms, in which signaling remains impaired despite apparent receptor recovery. Thus, Level II represents an additional layer of GPCR adaptation in which receptor desensitization is accompanied by longer-lasting remodeling of intracellular signaling capacity. Although derived primarily from D2-like and beta(2)-adrenergic receptor systems, this framework may apply more broadly to GPCRs capable of transactivating EGFR or related receptor tyrosine kinases, providing a broader view of desensitization that integrates receptor-level regulation with network-level adaptation. FAU - Kim, Kyeong-Man AU - Kim KM AD - Department of Pharmacology, College of Pharmacy, Chonnam National University, Gwangju 61186, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20261030 PL - Korea (South) TA - Biomol Ther (Seoul) JT - Biomolecules & therapeutics JID - 101472832 OTO - NOTNLM OT - Arrestin deubiquitination OT - EGFR transactivation OT - GPCR desensitization OT - Gbetagamma OT - Protein kinase C OT - USP33 EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 CRDT- 2026/10/29 02:33 PHST- 2026/06/10 00:00 [received] PHST- 2026/08/26 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:33 [entrez] AID - biomolther.2026.132 [pii] AID - 10.4062/biomolther.2026.132 [doi] PST - ppublish SO - Biomol Ther (Seoul). 2026 Nov 1;34(6):1283-1298. doi: 10.4062/biomolther.2026.132. Epub 2026 Oct 30. PMID- 42952195 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1098-2299 (Electronic) IS - 0272-4391 (Linking) VI - 87 IP - 7 DP - 2026 Nov TI - Recent Progress in HDAC-Based Dual-Target Inhibitors for Colorectal Cancer. LID - e70403 [pii] LID - 10.1002/ddr.70403 [doi] AB - Colorectal cancer (CRC) remains one of the leading causes of cancer-related incidence and mortality worldwide, with accumulating evidence indicating that its tumorigenesis and progression are tightly linked to epigenetic dysregulation. Histone deacetylases (HDACs) are key epigenetic regulators involved in gene transcription, cell-cycle regulation, and cancer progression, whereas the therapeutic benefits of conventional HDAC inhibitors are frequently compromised by limited selectivity and resistance development. Recently, dual-target HDAC inhibitors (HDACis) have attracted considerable attention as an emerging paradigm in anticancer drug development. These multitarget strategies can simultaneously enhance synergistic antitumor efficacy and alleviate the compensatory resistance commonly associated with single-target therapies via multi-mechanistic interventions. Accordingly, the concurrent modulation of HDACs and additional therapeutic targets represents a viable therapeutic strategy for CRC. In this review, we systematically summarize the recent progress of dual-target HDAC is in CRC treatment, with particular emphasis on their therapeutic potential and implications for future translational research and clinical practice. CI - (c) 2026 Wiley Periodicals LLC. FAU - Yang, Jiaqi AU - Yang J AUID- ORCID: 0009-0008-7459-2981 AD - College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, PR China. FAU - Shi, Xinyu AU - Shi X AD - College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, PR China. FAU - Xu, Yang AU - Xu Y AD - College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, PR China. FAU - Kong, Qinghe AU - Kong Q AD - College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, PR China. FAU - Kong, Degang AU - Kong D AD - College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, PR China. FAU - Feng, Yun AU - Feng Y AD - College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, PR China. FAU - Zhou, Honglei AU - Zhou H AUID- ORCID: 0000-0001-5539-4690 AD - College of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, PR China. LA - eng GR - SDYKC2024140/Shandong Provincial Graduate Student Excellence and Quality Course Project/ GR - LCKY202429/Clinical Research Special Project of Shandong University of Traditional Chinese Medicine/ PT - Journal Article PT - Review PL - United States TA - Drug Dev Res JT - Drug development research JID - 8204468 RN - 0 (Histone Deacetylase Inhibitors) RN - EC 3.5.1.98 (Histone Deacetylases) RN - 0 (Antineoplastic Agents) SB - IM MH - Humans MH - *Histone Deacetylase Inhibitors/therapeutic use/pharmacology/chemistry MH - *Colorectal Neoplasms/drug therapy/enzymology MH - Histone Deacetylases/metabolism MH - *Antineoplastic Agents/pharmacology/therapeutic use MH - Animals MH - Epigenesis, Genetic/drug effects OTO - NOTNLM OT - colorectal cancer OT - dual-target HDAC inhibitors OT - structure-activity relationship EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 CRDT- 2026/10/29 02:23 PHST- 2026/07/28 00:00 [revised] PHST- 2026/05/28 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:23 [entrez] AID - e70403 [pii] AID - 10.1002/ddr.70403 [doi] PST - ppublish SO - Drug Dev Res. 2026 Nov;87(7):e70403. doi: 10.1002/ddr.70403. PMID- 42952189 OWN - NLM STAT- Publisher LR - 20261029 IS - 1521-3773 (Electronic) IS - 1433-7851 (Linking) DP - 2026 Oct 28 TI - Germa-Michael Addition: Scope, Mechanism, and Access to Biocompatible Water-soluble Type I Photoinitiators. LID - e7379558 [pii] LID - 10.1002/anie.7379558 [doi] AB - Photoinitiators that operate efficiently in aqueous environments are essential for biomedical and materials applications. However, nontoxic photoinitiators that simultaneously combine high initiation efficiency, visible-light activity enabling sufficient curing depth, and adequate water solubility remain scarce. To overcome these limitations, we introduce a germanium-based, water-soluble photoinitiator prepared via the Germa-Michael addition. We also demonstrate the scope of this reaction and present the corresponding mechanism, which features complete regioselectivity. The aqueous solubility of the initiators is governed by PEG chain length, with full dissolution achieved at M(n) approximately 950 Da (compound 11). Photo-DSC benchmarking shows that 11 retains initiating efficacy comparable to the state-of-the-art germanium-based systems. Compound 11 displays good stability across acidic to basic pH values and exceptional stability in organic media. In HT29 cells, 11 shows low cytotoxicity up to 10 microM, with dose- and time-dependent effects at 50 microM, supporting biocompatibility in application-relevant ranges. The NHS-activated ester 7 enables selective bioconjugation to amines and amino acids. Additionally, we showcase a double Germa‑Michael addition on a single germanium center. Overall, this work positions Germa-Michael chemistry as a powerful entry to next-generation aqueous visible-light photoinitiators. CI - (c) 2026 The Author(s). Angewandte Chemie International Edition published by Wiley-VCH GmbH. FAU - Paris, Matthias AU - Paris M AD - Institute of Inorganic Chemistry, Graz University of Technology, Graz, Austria. AD - Christian Doppler Laboratory For New Semiconductor Materials based on Functionalized Hydrosilanes, Graz, Austria. FAU - Vorcnik, David AU - Vorcnik D AUID- ORCID: 0009-0005-1066-8463 AD - Institute of Inorganic Chemistry, Graz University of Technology, Graz, Austria. AD - Christian Doppler Laboratory For New Semiconductor Materials based on Functionalized Hydrosilanes, Graz, Austria. FAU - Raptova, Renata AU - Raptova R AD - Institute of Physical and Theoretical Chemistry, Graz University of Technology, Graz, Austria. FAU - Wiech, Mathias AU - Wiech M AD - Institute of Physical and Theoretical Chemistry, Graz University of Technology, Graz, Austria. FAU - Ansmann, Nils AU - Ansmann N AUID- ORCID: 0009-0002-6836-9835 AD - Institute of Inorganic Chemistry, Ruprecht-Karls Universitat Heidelberg, Heidelberg, Germany. FAU - Zhang, Zizheng AU - Zhang Z AUID- ORCID: 0009-0006-0250-1716 AD - Chair of Chemistry of Polymeric Materials, Montanuniversitat Leoben, Leoben, Austria. FAU - Torvisco, Ana AU - Torvisco A AUID- ORCID: 0000-0002-6203-7330 AD - Institute of Inorganic Chemistry, Graz University of Technology, Graz, Austria. FAU - Fischer, Roland C AU - Fischer RC AUID- ORCID: 0000-0001-9523-5010 AD - Institute of Inorganic Chemistry, Graz University of Technology, Graz, Austria. FAU - Bandl, Christine AU - Bandl C AUID- ORCID: 0000-0002-1110-0950 AD - Chair of Chemistry of Polymeric Materials, Montanuniversitat Leoben, Leoben, Austria. FAU - Fuchs, Julia AU - Fuchs J AUID- ORCID: 0000-0003-4576-615X AD - Division of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, Graz, Austria. AD - Institute of Biomechanics, Graz University of Technology, Graz, Austria. FAU - Brislinger, Dagmar AU - Brislinger D AUID- ORCID: 0000-0002-7319-5377 AD - Division of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, Graz, Austria. FAU - Greb, Lutz AU - Greb L AUID- ORCID: 0000-0002-5253-419X AD - Institute of Inorganic Chemistry, Ruprecht-Karls Universitat Heidelberg, Heidelberg, Germany. FAU - Gescheidt, Georg AU - Gescheidt G AUID- ORCID: 0000-0002-6827-4337 AD - Institute of Physical and Theoretical Chemistry, Graz University of Technology, Graz, Austria. FAU - Haas, Michael AU - Haas M AUID- ORCID: 0000-0002-9213-940X AD - Institute of Inorganic Chemistry, Graz University of Technology, Graz, Austria. AD - Christian Doppler Laboratory For New Semiconductor Materials based on Functionalized Hydrosilanes, Graz, Austria. LA - eng GR - 10.55776/I5710/Austrian Science Fund/ GR - 10.55776/PAT4237923/Austrian Science Fund/ GR - German Research Foundation/ GR - Austrian Federal Ministry of Labour and Economy/ GR - National Foundation for Research, Technology and Development/ GR - Marie Sklodowska-Curie Doctoral Networks/ PT - Journal Article DEP - 20261028 PL - Germany TA - Angew Chem Int Ed Engl JT - Angewandte Chemie (International ed. in English) JID - 0370543 SB - IM OTO - NOTNLM OT - biocompatibility OT - germanium OT - photochemistry OT - synthesis design OT - water-soluble photoinitiators EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 02:22 PHST- 2026/09/17 00:00 [revised] PHST- 2026/06/24 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:22 [entrez] AID - e7379558 [pii] AID - 10.1002/anie.7379558 [doi] PST - aheadofprint SO - Angew Chem Int Ed Engl. 2026 Oct 28:e7379558. doi: 10.1002/anie.7379558. PMID- 42952186 OWN - NLM STAT- Publisher LR - 20261029 IS - 1521-3773 (Electronic) IS - 1433-7851 (Linking) DP - 2026 Oct 28 TI - Metal-Free Reduction of CO(2) to Methanol Using Boranes at Ambient Conditions Promoted by Boron-Appended N-Heterocyclic Nitrenium Catalysts. LID - e3203227 [pii] LID - 10.1002/anie.3203227 [doi] AB - The chemical reduction of carbon dioxide (CO(2)) into value-added C1 building blocks, particularly under sustainable, metal-free conditions, remains a profound challenge in modern catalysis. Herein, we report robust boron-tethered N-heterocyclic nitrenium (NHN) catalysts for the reduction of CO(2) under ambient conditions to produce methoxide-level products. The developed protocol works under room temperature and 1 bar CO(2) pressure, with a low catalyst loading. The borane reductant governs the selectivity of the hydroboration reaction. Employing catecholborane (HBcat) or borane dimethylsulfide complex (BH(3).SMe(2)) drives rapid, selective, complete reduction to methoxide-level products. In contrast, the use of 9-borabicyclo[3.3.1]nonane (9-BBN) under identical conditions yields a mixture of acetal-level (CH(2)(OBBN)(2)) and methoxyborane (MeOBBN) species, while pinacolborane (HBpin) exhibits lower reactivity, generating a distribution across all three reduction states. Overall, the catalytic platform exhibits excellent turnover numbers (TON). This work establishes a sustainable, metal-free methodology for tunable reduction of CO(2) to methanol equivalents, highlighting the versatility of main-group catalysis. CI - (c) 2026 Wiley-VCH GmbH. FAU - Mondal, Rahul AU - Mondal R AUID- ORCID: 0009-0005-1387-342X AD - Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bengaluru, India. FAU - Mondal, Reetu Rani AU - Mondal RR AD - Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bengaluru, India. FAU - Geetharani, K AU - Geetharani K AUID- ORCID: 0000-0003-2064-1297 AD - Department of Inorganic and Physical Chemistry, Indian Institute of Science, Bengaluru, India. LA - eng GR - CRG/2023/004627/Science and Engineering Research Board (SERB)/ PT - Journal Article DEP - 20261028 PL - Germany TA - Angew Chem Int Ed Engl JT - Angewandte Chemie (International ed. in English) JID - 0370543 SB - IM OTO - NOTNLM OT - CO2 reduction OT - N-heterocyclic nitrenium ion OT - boranes OT - hydroboration OT - main-group catalysis EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 02:22 PHST- 2026/10/12 00:00 [revised] PHST- 2026/07/28 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:22 [entrez] AID - e3203227 [pii] AID - 10.1002/anie.3203227 [doi] PST - aheadofprint SO - Angew Chem Int Ed Engl. 2026 Oct 28:e3203227. doi: 10.1002/anie.3203227. PMID- 42952185 OWN - NLM STAT- Publisher LR - 20261029 IS - 1600-0714 (Electronic) IS - 0904-2512 (Linking) DP - 2026 Oct 28 TI - Familial Florid Cemento-Osseous Dysplasia: A Systematic Review Based on Contemporary Diagnostic Criteria. LID - 10.1111/jop.70193 [doi] AB - PURPOSE: Familial florid cemento-osseous dysplasia (FFCOD) is a rare fibro-osseous jaw disorder characterized by multifocal lesions and familial aggregation. This systematic review aimed to synthesize the evidence on the demographic, clinical, radiographic, histopathological, genetic, therapeutic, and follow-up characteristics of FFCOD. METHODS: A systematic review was conducted in accordance with PRISMA 2020 guidelines and registered in PROSPERO (CRD420261295922). Searches were performed in PubMed, Embase, Scopus, Web of Science, Cochrane Library, SciELO, LIVIVO, ProQuest, OpenGrey, IBICT/BDTD, Google Scholar, and reference lists through April 2025. Studies following the current World Health Organization (WHO) diagnostic criteria were eligible. Two independent reviewers performed study selection, data extraction, and risk-of-bias assessment using the Joanna Briggs Institute critical appraisal tool for case reports. RESULTS: Eight studies comprising 25 affected individuals were included. Females accounted for 60.0% of cases, and the mean age was 34.8 +/- 19.9 years. Multifocal involvement of both jaws (48.0%) and all four quadrants (32.0%) was common. All included cases exhibited a mixed radiolucent-radiopaque appearance (100.0%), while bone expansion was the most frequent clinical manifestation (33.3%). Histopathological examination consistently demonstrated fibrocellular stroma associated with cemento- and/or bone-like mineralized material. Molecular analysis was reported in one study and identified an ANO5 missense variant. Osteomyelitis, osteonecrosis, and bone exposure/sequestration were the most frequently reported complications. CONCLUSION: FFCOD demonstrates consistent clinicopathological characteristics, including multifocal jaw involvement, bone expansion, and a mixed radiolucent-radiopaque appearance. Although many individuals remain asymptomatic, infectious and necrotic complications may occur, emphasizing the importance of early recognition, careful treatment planning, and long-term clinical and radiographic follow-up. CI - (c) 2026 The Author(s). Journal of Oral Pathology & Medicine published by John Wiley & Sons Ltd. FAU - de Sousa Lopes Cascaes, Paula AU - de Sousa Lopes Cascaes P AUID- ORCID: 0009-0002-5684-6630 AD - Division of Oral Radiology, Faculdade Sao Leopoldo Mandic, Campinas, Sao Paulo, Brazil. FAU - Weigel, Sofia Junqueira AU - Weigel SJ AUID- ORCID: 0009-0004-4162-4474 AD - Division of Oral Radiology, Faculdade Sao Leopoldo Mandic, Campinas, Sao Paulo, Brazil. FAU - do Nascimento, Monikelly AU - do Nascimento M AUID- ORCID: 0000-0001-6765-0428 AD - Division of Oral Radiology, Faculdade Sao Leopoldo Mandic, Campinas, Sao Paulo, Brazil. FAU - Tavares, Gustavo Pereira AU - Tavares GP AUID- ORCID: 0009-0002-0050-1326 AD - Department of Surgery, Stomatology, Pathology and Radiology, Bauru School of Dentistry, University of Sao Paulo, Bauru, Brazil. FAU - Soares, Andresa Borges AU - Soares AB AUID- ORCID: 0000-0001-6112-3096 AD - Department of Surgery, Stomatology, Pathology and Radiology, Bauru School of Dentistry, University of Sao Paulo, Bauru, Brazil. FAU - Junqueira, Jose Luiz Cintra AU - Junqueira JLC AUID- ORCID: 0000-0001-6788-4021 AD - Division of Oral Radiology, Faculdade Sao Leopoldo Mandic, Campinas, Sao Paulo, Brazil. FAU - Soares, Mariana Quirino Silveira AU - Soares MQS AUID- ORCID: 0000-0002-9387-3729 AD - Division of Oral Radiology, Faculdade Sao Leopoldo Mandic, Campinas, Sao Paulo, Brazil. LA - eng PT - Journal Article DEP - 20261028 PL - Denmark TA - J Oral Pathol Med JT - Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology JID - 8911934 SB - IM OTO - NOTNLM OT - familial florid cemento-osseous dysplasia OT - fibro-osseous lesions OT - jaw diseases OT - systematic review EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 02:22 PHST- 2026/09/08 00:00 [revised] PHST- 2026/07/06 00:00 [received] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:22 [entrez] AID - 10.1111/jop.70193 [doi] PST - aheadofprint SO - J Oral Pathol Med. 2026 Oct 28. doi: 10.1111/jop.70193. PMID- 42952184 OWN - NLM STAT- Publisher LR - 20261029 IS - 1521-3773 (Electronic) IS - 1433-7851 (Linking) DP - 2026 Oct 28 TI - Chemoenzymatic Synthesis of 6-Sulfo Sialyl Lewis(x) Containing Glycans to Probe the Receptor Specificity of MERS Coronavirus. LID - e9067572 [pii] LID - 10.1002/anie.9067572 [doi] AB - The initial attachment of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) to host cell sialosides is critical for infection, yet its precise receptor specificity remains poorly understood. Here, we describe a chemoenzymatic methodology to synthesize a comprehensive panel of 6-sulfo sialyl Lewis(x) (6-sulfo-SLe(x)) containing glycans. Our approach involves enzymatically assembling an oligo-lactosamine chain modified at specific positions with N-trifluoroacetyl-glucosamine (GlcNTFA) moieties. Mild base treatment removes the TFA group to yield glucosamine, which effectively blocks enzymatic fucosylation. By combining this approach with the unique substrate selectivity of GlcNAc-6-O-sulfotransferases 2 (CHST-2), we selectively prepared fucosylated 6-sulfo-SLe(x) glycans. Microarray screening of these printed glycans revealed that a 6-sulfo-SLe(x) derivative presented on an extended LacNAc chain is the preferred host receptor for MERS-CoV. Conjugation of this compound to a polyglycerol-based dendrimer generated a multivalent inhibitor that potently blocks hemagglutination of human red blood cells by the MERS-CoV spike protein N-terminal domain (NTD). Furthermore, computational modeling demonstrated that the fucose moiety does not directly contact the viral spike protein. Instead, it pre-organizes the ligand into a favorable conformation, enabling a critical salt bridge between the glycan's sulfate group and the guanidinium side chain of viral residue Arg307. CI - (c) 2026 The Author(s). Angewandte Chemie International Edition published by Wiley-VCH GmbH. FAU - Wu, Yunfei AU - Wu Y AD - Division of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands. FAU - Kimpel, Anne L M AU - Kimpel ALM AUID- ORCID: 0009-0007-6172-8387 AD - Division of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands. FAU - van Trijp, Jacobus P AU - van Trijp JP AD - Division of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands. FAU - Uslu, Elif AU - Uslu E AUID- ORCID: 0009-0009-3678-1893 AD - Division of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands. FAU - Vos, Gasmall io, Russianl M AU - Vos GM AUID- ORCID: 0000-0002-4989-2367 AD - Division of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands. FAU - Unione, Luca AU - Unione L AD - Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Derio, Spain. AD - Ikerbasque, Basque Foundation for Science, Bilbao, Spain. FAU - de Vries, Robert P AU - de Vries RP AUID- ORCID: 0000-0002-1586-4464 AD - Division of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands. FAU - Boons, Geert-Jan AU - Boons GJ AUID- ORCID: 0000-0003-3111-5954 AD - Division of Chemical Biology and Drug Discovery, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, the Netherlands. AD - Complex Carbohydrate Research Center, Chemistry Department and Biochemistry Department, University of Georgia, Athens, Georgia, USA. AD - Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, the Netherlands. LA - eng GR - 101020769/ERC_/European Research Council/International GR - 101117639-Glyco13Cell/European Research Council ERC-2023-STG/ GR - PID2022-142639OA-I00/Agencia Estatal de Investigacion of Spain MCIN/AEI/ PT - Journal Article DEP - 20261028 PL - Germany TA - Angew Chem Int Ed Engl JT - Angewandte Chemie (International ed. in English) JID - 0370543 SB - IM OTO - NOTNLM OT - biological activity OT - carbohydrates OT - chemoenzymatic OT - enzymes OT - receptor specificity EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 02:22 PHST- 2026/10/16 00:00 [revised] PHST- 2026/05/04 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:22 [entrez] AID - e9067572 [pii] AID - 10.1002/anie.9067572 [doi] PST - aheadofprint SO - Angew Chem Int Ed Engl. 2026 Oct 28:e9067572. doi: 10.1002/anie.9067572. PMID- 42952180 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1860-7314 (Electronic) IS - 1860-6768 (Print) IS - 1860-6768 (Linking) VI - 21 IP - 11 DP - 2026 Nov TI - Engineering Mosaic T7 Bacteriophages for Dual Peptide Display to Enable Cellular Targeting and Ligand-Directed Protein Capture. LID - e70322 [pii] LID - 10.1002/biot.70322 [doi] AB - Integrating molecular recognition, tracing, and interactome capture within a single nanoparticle could allow for probing, manipulation, and control of complex biological systems. Achieving such multifunctionality within a single well-defined nanostructure remains challenging. T7 bacteriophage-based biological nanoparticles provide a robust and genetically programmable scaffold; however, conventional T7 display systems typically accommodate only one exogenous peptide species per phage particle, limiting multifunctional and combinatorial applications. Here, we describe a mosaic phage platform that enables the simultaneous display of two peptides on separate T7 coat protein subunits. By combining genome-encoded and plasmid-driven expression of peptide-fused capsid proteins, the system provides a modular route for assembling multifunctional phage nanoparticles. We generated mosaic T7 phages co-presenting the neuropilin-1 (NRP-1)-binding peptide RPARPAR alongside either a HA-tag or a Twin-Strep (Tstrep) tag affinity handle. These engineered phages specifically target NRP-1-expressing cells and enable immunofluorescence visualization and pull-down of NRP-1 from complex lysates. Furthermore, a random peptide T7 phage library co-displaying the constant lung-homing peptide RPARPAR shows preferential pulmonary accumulation in vivo, demonstrating utility for combinatorial targeting and tissue-directed phage selection. Collectively, this dual-display approach expands phage display by enabling cell targeting, visualization, molecular interactome probing, and in vivo tissue homing within a single genetically encoded nanoparticle platform. CI - (c) 2026 The Author(s). Biotechnology Journal published by Wiley-VCH GmbH. FAU - Marchetti, Luca AU - Marchetti L AUID- ORCID: 0000-0003-2234-5854 AD - Laboratory of Precision and Nanomedicine, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia. FAU - Posnograjeva, Kristina AU - Posnograjeva K AD - Laboratory of Precision and Nanomedicine, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia. FAU - Lingasamy, Prakash AU - Lingasamy P AUID- ORCID: 0000-0002-8826-4531 AD - Laboratory of Precision and Nanomedicine, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia. FAU - Pleiko, Karlis AU - Pleiko K AUID- ORCID: 0000-0002-1073-197X AD - Laboratory of Precision and Nanomedicine, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia. AD - National Institute of Research and Innovation, Riga, Latvia. FAU - Ilves, Ivar AU - Ilves I AUID- ORCID: 0000-0002-1747-9973 AD - Institute of Technology, University of Tartu, Tartu, Estonia. FAU - Teesalu, Tambet AU - Teesalu T AUID- ORCID: 0000-0002-9458-6385 AD - Laboratory of Precision and Nanomedicine, Institute of Biomedicine and Translational Medicine, University of Tartu, Tartu, Estonia. AD - Materials Research Laboratory, University of California, Santa Barbara, California, USA. LA - eng GR - 101182883/European Union's Horizon Europe/ GR - PRG230 PRG1788/Estonian Research Council/ GR - MOB3ERC121/Estonian Research Council/ GR - 101225036/ERC_/European Research Council/International GR - 2014-2020.4.01.15-0012/European Regional Development Fund/ GR - ECM-CART (EURONANOMED2021-113)/EuroNanoMed III/ GR - iNanoGun (EURONANOMED2020-071)/EuroNanoMed III/ GR - ReachGLIO (TRANSCAN2022-784-017)/TRANSCAN-3/ GR - 1.1.1.9/LZP/1/24/049 (AptaPhage)/Latvian Council of Science / European Regional Development Fund/ PT - Journal Article PL - Germany TA - Biotechnol J JT - Biotechnology journal JID - 101265833 RN - 0 (Peptide Library) RN - 0 (Capsid Proteins) RN - 0 (Peptides) RN - 0 (Ligands) RN - 144713-63-3 (Neuropilin-1) SB - IM MH - *Bacteriophage T7/genetics/metabolism MH - *Peptide Library MH - Capsid Proteins/genetics/metabolism MH - *Peptides/genetics/metabolism/chemistry MH - Ligands MH - Neuropilin-1/metabolism/genetics/chemistry MH - Nanoparticles/chemistry MH - Animals MH - Humans MH - Mice MH - *Cell Surface Display Techniques/methods PMC - PMC13717207 OTO - NOTNLM OT - T7 phage display OT - bacteriophage engineering OT - homing peptide OT - molecular targeting OT - multifunctional nanoparticles OT - protein pull-down OT - receptor identification COIS- Tambet Teesalu is the inventor of patents on iRGD and CendR peptides. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/29 CRDT- 2026/10/29 02:13 PHST- 2026/09/09 00:00 [revised] PHST- 2026/07/24 00:00 [received] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:13 [entrez] PHST- 2026/10/29 00:00 [pmc-release] AID - e70322 [pii] AID - BIOT70322 [pii] AID - 10.1002/biot.70322 [doi] PST - ppublish SO - Biotechnol J. 2026 Nov;21(11):e70322. doi: 10.1002/biot.70322. PMID- 42952179 OWN - NLM STAT- Publisher LR - 20261029 IS - 1522-1601 (Electronic) IS - 0161-7567 (Linking) DP - 2026 Oct 28 TI - Pulsatile Perfusion Therapy Protects Internal Carotid Artery Blood Flow and Cerebrovascular Blood Oxygen Saturation with Simulated Hemorrhage in Humans. LID - 10.1152/japplphysiol.90377.2026 [doi] AB - Pulsatile Perfusion Therapy (PPT), a method we developed to induce 0.1 Hz hemodynamic oscillations (10-second cycles), protects blood oxygen saturation during ischemia. PPT via oscillatory lower body negative pressure (LBNP) protected cerebrovascular blood oxygen saturation (ScO(2)) during simulated hemorrhage in humans (induced via LBNP). Thigh cuff compressions are a new approach to achieve similar 0.1 Hz hemodynamic oscillations. We hypothesized that PPT via thigh cuff compressions would attenuate reductions in cerebral blood flow and cerebrovascular blood oxygen saturation during LBNP in humans. Fifteen healthy humans (6F/9M; age, 25.8 +/- 4.5 y) completed two protocols of -60 mmHg LBNP. In the PPT condition, thigh cuffs intermittently inflated and deflated in 10-s cycles during the LBNP protocol. In the control condition (CON), the thigh cuffs remained deflated. Internal carotid artery (ICA) blood flow and ScO(2) were measured continuously. PPT attenuated reductions in ICA blood flow (PPT: -17.6 +/- 11.3% vs. CON: -31.0 +/- 11.8%; P=0.02) and ScO(2) (PPT: -5.2 +/- 2.4% vs. CON: -7.1 +/- 3.6%; P=0.07) compared to CON. These data provide evidence for PPT as a potential intervention for the treatment of cerebral hypoperfusion during hemorrhage. FAU - Dinh, Viet Q AU - Dinh VQ AUID- ORCID: 0000-0001-6472-8648 AD - Cerebral & Cardiovascular Physiology Laboratory, Department of Physiology & Anatomy, University of North Texas Health Science Center, Fort Worth, TX, USA. ROR: https://ror.org/05msxaq47 FAU - Davis, K Austin AU - Davis KA AUID- ORCID: 0000-0002-1277-6266 AD - Cerebral & Cardiovascular Physiology Laboratory, Department of Physiology & Anatomy, University of North Texas Health Science Center, Fort Worth, TX, USA. ROR: https://ror.org/05msxaq47 AD - Applied Physiology and Advanced Imaging Laboratory, Department of Kinesiology, The University of Texas at Arlington, Arlington, TX, USA. FAU - Peralez, Lindsey AU - Peralez L AD - Cerebral & Cardiovascular Physiology Laboratory, Department of Physiology & Anatomy, University of North Texas Health Science Center, Fort Worth, TX, USA. ROR: https://ror.org/05msxaq47 FAU - Rickards, Caroline A AU - Rickards CA AUID- ORCID: 0000-0002-5759-6912 AD - Cerebral & Cardiovascular Physiology Laboratory, Department of Physiology & Anatomy, University of North Texas Health Science Center, Fort Worth, TX, USA. ROR: https://ror.org/05msxaq47 LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Appl Physiol (1985) JT - Journal of applied physiology (Bethesda, Md. : 1985) JID - 8502536 SB - IM OTO - NOTNLM OT - 0.1 Hz OT - cerebral blood flow OT - cerebrovascular blood oxygen saturation OT - hemodynamic oscillations OT - lower body negative pressure EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 02:13 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:13 [entrez] AID - 10.1152/japplphysiol.90377.2026 [doi] PST - aheadofprint SO - J Appl Physiol (1985). 2026 Oct 28. doi: 10.1152/japplphysiol.90377.2026. PMID- 42952178 OWN - NLM STAT- Publisher LR - 20261029 IS - 2198-3844 (Electronic) IS - 2198-3844 (Linking) DP - 2026 Oct 28 TI - Propofol Modulates Fear Memory Reconsolidation by Inhibiting D2 Receptor-Expressing Neurons in the Medial Prefrontal Cortex. LID - e78527 [pii] LID - 10.1002/advs.78527 [doi] AB - Fear memory reconsolidation provides a temporal window for modifying established fear memories, yet the underlying cellular and neural circuit mechanisms remain incompletely understood. Using in vivo fiber photometry and chemogenetic approaches in freely moving mice, we show that propofol modulates fear memory reconsolidation by inhibiting D2 receptor-expressing neurons in the medial prefrontal cortex (mPFC). These neurons exhibit heightened activity during fear memory reconsolidation and project prominently to the basolateral amygdala (BLA). Notably, local pharmacological activation of D2 receptors in the mPFC recapitulates the fear-attenuating effect of propofol when administered during the reconsolidation window. Circuit-level investigations further show that propofol suppresses the reconsolidation-associated enhancement of the mPFC(D2)-BLA synaptic transmission, and chemogenetic activation of this projection counteracts the effect of propofol on fear memory reconsolidation. Moreover, selective knockdown of D2 receptors in BLA-projecting mPFC neurons diminishes the effect of propofol, supporting a critical role for D2 receptor signaling within this projection-defined circuit. Together, these findings identify the mPFC(D2)-BLA circuit as a key neural substrate through which propofol modulates fear memory reconsolidation and highlight prefrontal D2 receptor signaling as a potential target for reconsolidation-based interventions aimed at maladaptive fear memories. CI - (c) 2026 The Author(s). Advanced Science published by Wiley-VCH GmbH. FAU - Li, Jie AU - Li J AD - Shanghai Key Laboratory of Mental Disorders Translational Research, Brain Health Institute, National Center for Mental Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine and School of Psychology, Shanghai, China. AD - School of Life Science and Technology, ShanghaiTech University, Shanghai, China. AD - Department of Psychiatry, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China. FAU - Guo, Yi-Ge AU - Guo YG AD - School of Life Science and Technology, ShanghaiTech University, Shanghai, China. FAU - Xu, Xing AU - Xu X AD - Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Meng, Gui-Lin AU - Meng GL AUID- ORCID: 0000-0002-4531-3938 AD - Department of Neurology, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Li, Han AU - Li H AD - Shanghai Key Laboratory of Mental Disorders Translational Research, Brain Health Institute, National Center for Mental Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine and School of Psychology, Shanghai, China. FAU - Chu, Liu-Wei AU - Chu LW AD - School of Life Science and Technology, ShanghaiTech University, Shanghai, China. FAU - Chen, Xin-Yuan AU - Chen XY AD - School of Life Science and Technology, ShanghaiTech University, Shanghai, China. FAU - Guo, Xiao-Yun AU - Guo XY AUID- ORCID: 0000-0003-2738-3868 AD - Shanghai Key Laboratory of Mental Disorders Translational Research, Brain Health Institute, National Center for Mental Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine and School of Psychology, Shanghai, China. FAU - Xu, Tao AU - Xu T AUID- ORCID: 0000-0001-5868-4079 AD - Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Yuan, Ti-Fei AU - Yuan TF AUID- ORCID: 0000-0003-0510-715X AD - Shanghai Key Laboratory of Mental Disorders Translational Research, Brain Health Institute, National Center for Mental Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine and School of Psychology, Shanghai, China. FAU - Hu, Ji AU - Hu J AD - School of Life Science and Technology, ShanghaiTech University, Shanghai, China. FAU - Zhu, Xiao-Na AU - Zhu XN AUID- ORCID: 0009-0008-3876-7975 AD - Shanghai Key Laboratory of Mental Disorders Translational Research, Brain Health Institute, National Center for Mental Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine and School of Psychology, Shanghai, China. LA - eng GR - 2025YFC2511300/National Key R&D Program of China/ GR - 82522034/National Natural Science Foundation of China/ GR - 82371522/National Natural Science Foundation of China/ GR - YG2024ZD25/Sponsored by the Fundamental Research Funds for the Central Universities/ GR - GZC20251512/Postdoctoral Fellowship Program of China Postdoctoral Science Foundation/ GR - gyfybsky-2026-13/Doctoral Research Startup Fund of the Affiliated Hospital of Guizhou Medical University/ PT - Journal Article DEP - 20261028 PL - Germany TA - Adv Sci (Weinh) JT - Advanced science (Weinheim, Baden-Wurttemberg, Germany) JID - 101664569 SB - IM PMC - PMC13717115 OTO - NOTNLM OT - D2 receptors OT - D2 receptor-expressing neurons OT - fear memory reconsolidation OT - mPFCD2-BLA pathway OT - medial prefrontal cortex (mPFC) OT - propofol COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 PMCR- 2026/10/28 CRDT- 2026/10/29 02:13 PHST- 2026/10/14 00:00 [revised] PHST- 2026/09/09 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:13 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e78527 [pii] AID - ADVS78527 [pii] AID - 10.1002/advs.78527 [doi] PST - aheadofprint SO - Adv Sci (Weinh). 2026 Oct 28:e78527. doi: 10.1002/advs.78527. PMID- 42952177 OWN - NLM STAT- Publisher LR - 20261029 IS - 1548-9221 (Electronic) IS - 1548-9221 (Linking) DP - 2026 Oct 28 TI - Central Command During Exercise: Hierarchical Integration of Feedforward and Feedback Cardiovascular Control. LID - 10.1152/physiol.90017.2026 [doi] AB - Cardiovascular regulation during exercise has traditionally been explained by the interaction between central command, a feedforward neural mechanism originating in higher brain centers, and peripheral cardiovascular reflexes, particularly the exercise pressor reflex and arterial baroreflex. Although central command is widely recognized as initiating autonomic adjustments in proportion to perceived effort, accumulating evidence indicates that its role extends well beyond the generation of parallel autonomic drive. In this review, we synthesize experimental findings spanning spinal and brainstem neurophysiology to integrative studies in intact animals and humans, showing that descending signals associated with central command may access the neural circuitry involved in processing peripheral cardiovascular information and regulating autonomic output. Neurophysiological studies identify multiple sites of interaction, including inhibitory gating of group III/IV muscle afferent transmission within the spinal dorsal horn and modulation of barosensory processing within the nucleus of the solitary tract. At the integrative level, studies of the arterial baroreflex, exercise pressor reflex, and trigeminally mediated diving reflex further demonstrate that central command can selectively regulate reflex expression and modify interactions among competing autonomic inputs rather than summating with them. Together, these observations support a conceptual evolution of central command from a feedforward initiator to a hierarchical autonomic organizing system (CHAOS) that dynamically integrates central command with peripheral cardiovascular feedback according to behavioral context. This CHAOS framework provides a unifying physiological interpretation for findings traditionally considered within individual reflex systems and offers a broader perspective on cardiovascular regulation during exercise and other conditions imposing competing autonomic demands. FAU - Araujo, Marcela S AU - Araujo MS AD - NeuroV̇ASQ̇ - Integrative Physiology Laboratory, Faculty of Physical Education, University of Brasilia, Brasilia, DF, Brazil. ROR: https://ror.org/02xfp8v59 AD - National Institute of Science and Technology-INCT, Physical (In)activity and Health, CNPq, Brasilia, Brazil. FAU - Mizuno, Masaki AU - Mizuno M AUID- ORCID: 0000-0003-4455-8084 AD - Department of Applied Clinical Research, UT Southwestern Medical Center, Dallas, Texas, USA. FAU - Silva, Bruno M AU - Silva BM AD - National Institute of Science and Technology-INCT, Physical (In)activity and Health, CNPq, Brasilia, Brazil. AD - Department of Physiology, Federal University of Sao Paulo, Sao Paulo, Brazil. FAU - Vianna, Lauro C AU - Vianna LC AUID- ORCID: 0000-0002-5747-0295 AD - NeuroV̇ASQ̇ - Integrative Physiology Laboratory, Faculty of Physical Education, University of Brasilia, Brasilia, DF, Brazil. ROR: https://ror.org/02xfp8v59 AD - National Institute of Science and Technology-INCT, Physical (In)activity and Health, CNPq, Brasilia, Brazil. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Physiology (Bethesda) JT - Physiology (Bethesda, Md.) JID - 101208185 SB - IM OTO - NOTNLM OT - arterial baroreflex OT - autonomic regulation OT - cardiovascular reflexes OT - exercise pressor reflex OT - neural integration EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 02:13 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:13 [entrez] AID - 10.1152/physiol.90017.2026 [doi] PST - aheadofprint SO - Physiology (Bethesda). 2026 Oct 28. doi: 10.1152/physiol.90017.2026. PMID- 42952173 OWN - NLM STAT- Publisher LR - 20261029 IS - 2198-3844 (Electronic) IS - 2198-3844 (Linking) DP - 2026 Oct 28 TI - An Adipocyte MKK3-p38beta Axis Preserves Insulin Responsiveness in Mice and Is Reciprocally Altered in Human Obesity. LID - e20388 [pii] LID - 10.1002/advs.202520388 [doi] AB - Obesity is a major risk factor for insulin resistance and type 2 diabetes. Adipocyte stress responses can either promote metabolic dysfunction or preserve insulin action, but the contribution of the p38 MAPK activator MKK3 to adipose tissue physiology remains poorly understood. Here, we show that adipose MKK3 expression increases early during high-fat feeding and is also elevated in human obesity. In human visceral adipose tissue, MKK3 expression was positively associated with body mass index and leptin and inversely associated with HbA1c in unadjusted analyses. Using germline, Fabp4-Cre, and Adipo-Cre Mkk3-deficient mice, together with p38beta-deficient mice and primary adipocytes, we found that loss of adipocyte MKK3 impairs insulin responsiveness. Adipo-Cre;Mkk3(fl/fl) (Adipo(Mkk3)) mice displayed reduced insulin-stimulated glucose uptake selectively in subcutaneous white adipose tissue despite unchanged body composition and basal brown adipose tissue activity. Mechanistically, MKK3 or p38beta deficiency increased p70S6K activity and inhibitory IRS1 Ser307 phosphorylation, reduced GLUT4 translocation and Akt activation, and these defects were partially rescued by rapamycin or the selective p70S6K inhibitor PF-4708671. Together, these findings identify an adipocyte MKK3-p38beta-p70S6K axis that helps preserve insulin signaling during nutritional stress. CI - (c) 2026 The Author(s). Advanced Science published by Wiley-VCH GmbH. FAU - Bernardo, Edgar AU - Bernardo E AUID- ORCID: 0000-0002-0500-9632 AD - Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain. FAU - Matesanz, Nuria AU - Matesanz N AD - Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid, Spain. FAU - Torres, Jorge-Luis AU - Torres JL AD - Complejo Asistencial de Zamora, Zamora, Spain. FAU - Arcones, Alba C AU - Arcones AC AUID- ORCID: 0000-0001-8182-4973 AD - Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain. FAU - Jimenez-Loygorri, Juan Ignacio AU - Jimenez-Loygorri JI AUID- ORCID: 0000-0002-3065-9952 AD - Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain. FAU - Mora, Alfonso AU - Mora A AUID- ORCID: 0000-0002-6397-4836 AD - Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain. FAU - Herrera-Melle, Leticia AU - Herrera-Melle L AD - Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid, Spain. FAU - Leiva-Vega, Luis AU - Leiva-Vega L AUID- ORCID: 0000-0002-0050-8117 AD - Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain. FAU - Rodriguez, Maria Elena AU - Rodriguez ME AD - Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain. FAU - Hernandez-Cosido, Lourdes AU - Hernandez-Cosido L AUID- ORCID: 0000-0003-1359-7478 AD - Bariatric Surgery Unit, Department of General Surgery, University Hospital of Salamanca, Department of Surgery, University of Salamanca, Salamanca, Spain. FAU - Leon, Marta AU - Leon M AUID- ORCID: 0009-0005-4170-341X AD - Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain. FAU - Gonzalez, Paula Yong AU - Gonzalez PY AD - Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain. FAU - Duran, Teresa AU - Duran T AUID- ORCID: 0009-0006-7153-4343 AD - Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain. FAU - Parracho, Tamara AU - Parracho T AUID- ORCID: 0000-0001-9283-4464 AD - Department of Physiology, CiMUS, University of Santiago de Compostela, Santiago de Compostela, Spain. FAU - Mulero, Francisca AU - Mulero F AUID- ORCID: 0000-0001-9584-4211 AD - Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain. FAU - Nogueiras, Ruben AU - Nogueiras R AUID- ORCID: 0000-0002-9976-9930 AD - Department of Physiology, CiMUS, University of Santiago de Compostela, Santiago de Compostela, Spain. FAU - Arthur, J Simon C AU - Arthur JSC AUID- ORCID: 0000-0002-8135-1958 AD - Division of Cell Signalling and Immunology, Faculty of Life Sciences, University of Dundee, Dundee, UK. FAU - Nebreda, Angel R AU - Nebreda AR AUID- ORCID: 0000-0002-7631-4060 AD - Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Barcelona, Spain. AD - Institucio Catalana de Recerca i Estudis Avancats (ICREA), Barcelona, Spain. FAU - Davis, Roger J AU - Davis RJ AUID- ORCID: 0000-0002-0130-1652 AD - Program in Molecular Medicine, University of Massachusetts Chan Medical School, Massachusetts, USA. FAU - Marcos, Miguel AU - Marcos M AUID- ORCID: 0000-0003-1269-4487 AD - Department of Internal Medicine, University Hospital of Salamanca-IBSAL, Salamanca, Spain. AD - Department of Medicine, University of Salamanca, Salamanca, Spain. FAU - Sabio, Guadalupe AU - Sabio G AUID- ORCID: 0000-0002-2822-0625 AD - Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain. LA - eng GR - SAF2010-19347/Ministerio de Economia y Competitividad/ GR - excellence2024_22/CRIS Contra el Cancer/ GR - PID2019-104399RB-I00/Ministerio de Ciencia e Innovacion/ GR - PMP21/00113/Instituto de Salud Carlos III/ GR - PI20/00743/Instituto de Salud Carlos III/ GR - INT21/00065/Instituto de Salud Carlos III/ GR - FPU15 05802/Ministerio de Educacion, Cultura y Deporte/ GR - Sociedad Espanola de Medicina Interna (SEMI)/ GR - GRS2916/A1/2023/Junta de Castilla y Leon/ GR - GRS2388/A/21/Junta de Castilla y Leon/ GR - GRS2648/A/22/Junta de Castilla y Leon/ GR - ERC Synergy Grant AdipoHealth/ERC_/European Research Council/International GR - SYG2024/SAL-GL-1012/Comunidad de Madrid CAM-SINERGICOS 2024/ GR - LCF/PR/HR24/52440001/La Caixa Foundation/ GR - IMPACT-2021/Infraestructura de Medicina de Precision asociada a la Ciencia y Tecnologia/ GR - SOCALMI2022/Sociedad Castellano-Leonesa y Cantabra de Medicina Interna/ GR - SEV-2015-0505/Severo Ochoa Center of Excellence/ GR - S2022/BMD-7403(RENIM-CM)/Comunidad de Madrid/ GR - CA1/RSUE/2021-00614/Margarita salas/ GR - FJC2020-043126-I/Juan de la Cierva Program/ GR - POSTD2610149JIME/Fundacion Cientifica Asociacion Espanola Contra el Cancer/ GR - CNIO Friends/ PT - Journal Article DEP - 20261028 PL - Germany TA - Adv Sci (Weinh) JT - Advanced science (Weinheim, Baden-Wurttemberg, Germany) JID - 101664569 SB - IM PMC - PMC13717117 OTO - NOTNLM OT - MKK3/p38beta signaling OT - adipocytes OT - insulin resistance OT - obesity OT - type 2 diabetes mellitus COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 PMCR- 2026/10/28 CRDT- 2026/10/29 02:12 PHST- 2026/08/12 00:00 [revised] PHST- 2025/10/16 00:00 [received] PHST- 2026/09/07 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:12 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e20388 [pii] AID - ADVS77744 [pii] AID - 10.1002/advs.202520388 [doi] PST - aheadofprint SO - Adv Sci (Weinh). 2026 Oct 28:e20388. doi: 10.1002/advs.202520388. PMID- 42952171 OWN - NLM STAT- Publisher LR - 20261029 IS - 1098-2353 (Electronic) IS - 0897-3806 (Linking) DP - 2026 Oct 28 TI - The Fibular Artery Communicating Branch and Fibular Artery Dominance: An Anatomical Hazard for Posterior Approaches to the Ankle. LID - 10.1002/ca.70248 [doi] AB - Open reduction of posterior malleolar fractures (PMFs) increasingly employs direct posterior approaches. The posterolateral and posteromedial approaches both develop the deep posterior compartment in the plane between the fibular and posterior tibial arteries, placing the fibular artery communicating branch (FACB) within the operative field; inadvertent injury to this branch has been associated with limb-threatening ischemia. This study quantified the prevalence of the FACB and of fibular artery dominance (FAD), a configuration in which the fibular artery provides the principal distal supply because the posterior tibial artery is atretic or absent, and considered the implications for surgical approach selection. In a single-center cross-sectional study, 916 consecutive lower-limb computed tomography angiograms performed over 1 year were screened; 726 met the inclusion criteria and were assessed by trained reviewers for the FACB, FAD, laterality and clinical indication. An FACB was identified in 280 patients (38.6%, 95% CI 35.1-42.2). Its prevalence varied with clinical indication (chi(2) = 33.67, p < 0.001), being highest in peripheral vascular disease (45.1%) and lowest in other vascular referrals (16.4%). FAD occurred exclusively in FACB-positive limbs, in 28 patients (10.0% of FACB-positive limbs; 3.9% of the whole cohort, 95% CI 2.7-5.5), and its distribution did not differ by indication (Freeman-Halton exact p = 0.81). Because the FACB lies within the field of the posterolateral and posteromedial approaches but posterior and lateral to the medial-posteromedial (MPM) approach, the MPM approach avoids the at-risk plane. These findings support anatomically informed approach selection and caution when a posterolateral or posteromedial approach is planned, particularly in patients with peripheral vascular disease. CI - (c) 2026 The Author(s). Clinical Anatomy published by Wiley Periodicals LLC on behalf of American Association of Clinical Anatomists and British Association of Clinical Anatomists. FAU - Johnston McCarter, Annabelle AU - Johnston McCarter A AUID- ORCID: 0000-0003-1763-4643 AD - University Hospitals of Liverpool Group, Liverpool, UK. AD - University of Liverpool, Liverpool, UK. FAU - Orji, Chijioke AU - Orji C AD - University Hospitals of Liverpool Group, Liverpool, UK. FAU - Wyatt, Craig AU - Wyatt C AD - University Hospitals of Liverpool Group, Liverpool, UK. FAU - Aslam, Shahjahan AU - Aslam S AD - University Hospitals of Liverpool Group, Liverpool, UK. FAU - Wyman, Emily AU - Wyman E AUID- ORCID: 0000-0002-8196-4922 AD - University Hospitals of Liverpool Group, Liverpool, UK. FAU - El-Gheryani, Muhaned AU - El-Gheryani M AUID- ORCID: 0009-0004-5937-8849 AD - University Hospitals of Liverpool Group, Liverpool, UK. FAU - Akbar, Zeeshan AU - Akbar Z AD - University Hospitals of Liverpool Group, Liverpool, UK. FAU - Gala, Kosha AU - Gala K AD - University Hospitals of Liverpool Group, Liverpool, UK. FAU - Mason, Lyndon AU - Mason L AD - University Hospitals of Liverpool Group, Liverpool, UK. AD - University of Liverpool, Liverpool, UK. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Clin Anat JT - Clinical anatomy (New York, N.Y.) JID - 8809128 SB - IM OTO - NOTNLM OT - anatomical variation OT - ankle fractures OT - computed tomography angiography OT - fibular artery OT - posterior tibial artery OT - surgical approach EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 02:12 PHST- 2026/10/11 00:00 [revised] PHST- 2026/08/14 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:12 [entrez] AID - 10.1002/ca.70248 [doi] PST - aheadofprint SO - Clin Anat. 2026 Oct 28. doi: 10.1002/ca.70248. PMID- 42952168 OWN - NLM STAT- Publisher LR - 20261029 IS - 2198-3844 (Electronic) IS - 2198-3844 (Linking) DP - 2026 Oct 28 TI - BRCA1 Mutation-Recognizing Tetrahedral Framework Nucleic Acid Nanoplatform Enables Coordinated PARP/WEE1 Inhibition and STING-Associated Antitumor Immunity in Ovarian Cancer. LID - e78457 [pii] LID - 10.1002/advs.78457 [doi] AB - Improving therapeutic efficacy and antitumor immune responsiveness in BRCA1-mutant ovarian cancer remains challenging. Here, we developed a BRCA1 mutation-guided tetrahedral framework nucleic acid nanosystem, FBOA-tFNA, for coordinated intracellular delivery of the PARP inhibitor olaparib and the WEE1 inhibitor adavosertib. After folate receptor-mediated uptake, FBOA-tFNA recognizes BRCA1 mutant RNA through toehold-mediated strand displacement, enabling mutation-selective beacon activation, while drug release is independently regulated by reduction-responsive disulfide cleavage. Coordinated PARP/WEE1 inhibition amplified DNA damage, impaired G2/M checkpoint protection, and promoted tumor-cell killing in BRCA1-mutant ovarian cancer models. Resulting DNA damage-associated intracellular dsDNA signals activated STING-associated signaling, type I interferon responses, immunogenic cell death (ICD)-associated stress, dendritic-cell maturation, and CD8(+) T-cell activation. Engineered patient-derived ovarian cancer organoids expressing a BRCA1(2594delC)-containing transcript further supported the mutation-selective recognition and therapeutic potential of FBOA-tFNA ex vivo. In vivo, FBOA-tFNA showed tumor-associated fluorescence accumulation, BRCA1 mutant-transcript recognition-associated beacon activation, enhanced antitumor efficacy, and preliminary repeated-dose tolerability. Moreover, FBOA-tFNA enhanced antitumor immune responses and improved the response to anti-PD-1 therapy, accompanied by increased effector and memory-like T-cell populations. This programmable DNA nanoplatform provides a personalized combination strategy for coordinated DNA-damage therapy and immunomodulation in BRCA1-mutant ovarian cancer. CI - (c) 2026 The Author(s). Advanced Science published by Wiley-VCH GmbH. FAU - Wang, Chenyi AU - Wang C AUID- ORCID: 0000-0003-0451-5346 AD - Laboratory of Gynecologic Oncology, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Maternity and Child Health Hospital, Fujian Medical University, Fuzhou, China. AD - Fujian Clinical Research Center for Gynecological Oncology, Fujian Maternity and Child Health Hospital (Fujian Obstetrics and Gynecology Hospital), Fuzhou, China. AD - Fujian Key Laboratory of Women and Children's Critical Diseases Research, Fuzhou, China. AD - School of Medicine, Fuzhou University, Fuzhou, China. AD - MOE Key Laboratory For Analytical Science of Food Safety and Biology, Fujian Key Laboratory of Analysis and Testing Technology For Food Safety and Health, College of Chemistry, Fuzhou University, Fuzhou, China. FAU - Yao, Mengying AU - Yao M AD - MOE Key Laboratory For Analytical Science of Food Safety and Biology, Fujian Key Laboratory of Analysis and Testing Technology For Food Safety and Health, College of Chemistry, Fuzhou University, Fuzhou, China. FAU - Xie, Jinhui AU - Xie J AD - Laboratory of Gynecologic Oncology, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Maternity and Child Health Hospital, Fujian Medical University, Fuzhou, China. AD - Fujian Clinical Research Center for Gynecological Oncology, Fujian Maternity and Child Health Hospital (Fujian Obstetrics and Gynecology Hospital), Fuzhou, China. AD - Fujian Key Laboratory of Women and Children's Critical Diseases Research, Fuzhou, China. AD - School of Medicine, Fuzhou University, Fuzhou, China. FAU - Wang, Dingjie AU - Wang D AUID- ORCID: 0009-0002-4577-9567 AD - Laboratory of Gynecologic Oncology, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Maternity and Child Health Hospital, Fujian Medical University, Fuzhou, China. AD - Fujian Clinical Research Center for Gynecological Oncology, Fujian Maternity and Child Health Hospital (Fujian Obstetrics and Gynecology Hospital), Fuzhou, China. AD - Fujian Key Laboratory of Women and Children's Critical Diseases Research, Fuzhou, China. FAU - Shi, Yashi AU - Shi Y AUID- ORCID: 0009-0002-4133-7761 AD - Laboratory of Gynecologic Oncology, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Maternity and Child Health Hospital, Fujian Medical University, Fuzhou, China. AD - Fujian Clinical Research Center for Gynecological Oncology, Fujian Maternity and Child Health Hospital (Fujian Obstetrics and Gynecology Hospital), Fuzhou, China. AD - Fujian Key Laboratory of Women and Children's Critical Diseases Research, Fuzhou, China. FAU - Mao, Xiaodan AU - Mao X AUID- ORCID: 0000-0001-8242-914X AD - Laboratory of Gynecologic Oncology, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Maternity and Child Health Hospital, Fujian Medical University, Fuzhou, China. AD - Fujian Clinical Research Center for Gynecological Oncology, Fujian Maternity and Child Health Hospital (Fujian Obstetrics and Gynecology Hospital), Fuzhou, China. AD - Fujian Key Laboratory of Women and Children's Critical Diseases Research, Fuzhou, China. FAU - Lu, Chunhua AU - Lu C AUID- ORCID: 0000-0001-7725-885X AD - School of Medicine, Fuzhou University, Fuzhou, China. AD - MOE Key Laboratory For Analytical Science of Food Safety and Biology, Fujian Key Laboratory of Analysis and Testing Technology For Food Safety and Health, College of Chemistry, Fuzhou University, Fuzhou, China. FAU - Sun, Pengming AU - Sun P AUID- ORCID: 0000-0002-5072-6091 AD - Laboratory of Gynecologic Oncology, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Maternity and Child Health Hospital, Fujian Medical University, Fuzhou, China. AD - Fujian Clinical Research Center for Gynecological Oncology, Fujian Maternity and Child Health Hospital (Fujian Obstetrics and Gynecology Hospital), Fuzhou, China. AD - Fujian Key Laboratory of Women and Children's Critical Diseases Research, Fuzhou, China. AD - School of Medicine, Fuzhou University, Fuzhou, China. LA - eng GR - 2026QNGGB009/Fujian Provincial Traditional Chinese Medicine Science and Technology Project Funding Program/ GR - 82674160/National Natural Science Foundation of China/ GR - 2026J0011280/Fujian Provincial Natural Science Foundation/ GR - 2026J002019/Fujian Provincial Natural Science Foundation/ GR - YCXY25-04/Fujian Provincial Medical Peak Excellence Initiative; and the 2025 Science and Technology Innovation Start-up Fund of Fujian Maternity and Child Health Hospital/ PT - Journal Article DEP - 20261028 PL - Germany TA - Adv Sci (Weinh) JT - Advanced science (Weinheim, Baden-Wurttemberg, Germany) JID - 101664569 SB - IM PMC - PMC13717119 OTO - NOTNLM OT - BRCA1 mutation OT - PARP/WEE1 inhibitor OT - STING signaling OT - ovarian cancer OT - synthetic lethality COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 PMCR- 2026/10/28 CRDT- 2026/10/29 02:04 PHST- 2026/10/13 00:00 [revised] PHST- 2026/07/07 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:04 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e78457 [pii] AID - ADVS78457 [pii] AID - 10.1002/advs.78457 [doi] PST - aheadofprint SO - Adv Sci (Weinh). 2026 Oct 28:e78457. doi: 10.1002/advs.78457. PMID- 42952160 OWN - NLM STAT- Publisher LR - 20261029 IS - 1528-0691 (Electronic) IS - 1528-0691 (Linking) DP - 2026 Oct 28 TI - Gold Meets Mechanochemistry: Emerging Opportunities in Organometallics and Catalytic Reactions. LID - e70266 [pii] LID - 10.1002/tcr.70266 [doi] AB - Mechanochemistry has recently emerged as a practical approach for accessing gold-based organometallic complexes under solvent-minimized or solvent-free conditions. In this personal account, I summarize the developing intersection between gold chemistry and ball-milling, with emphasis on the preparation of Au(I) and Au(III) complexes bearing N-heterocyclic carbene, acyclic diaminocarbene, phosphine, pincer, and cyclometalated ligands. It also discusses the still limited but conceptually important use of gold species in mechanochemical catalysis, including C-H functionalization, multicomponent reactions, alcohol oxidation, and nitroarene reduction. Beyond listing synthetic protocols, this account highlights the operational advantages, current limitations, and unresolved mechanistic questions that define this emerging area. Notable limitations include lack of mechanistic understanding, less informative green metric analysis, unknown reproducibility studies, and scale-up opportunities. The available studies indicate that mechanochemistry can simplify access to selected gold complexes and enable distinctive solid-state catalytic manifolds; however, broader substrate scope, standardized milling parameters, rigorous green metrics, and deeper mechanistic insight will be essential for the field to mature. CI - (c) 2026 The Chemical Society of Japan and Wiley-VCH GmbH. FAU - Praveen, Chandrasekar AU - Praveen C AUID- ORCID: 0000-0001-6999-6801 AD - Synthesis and Enabling Chemical Technologies Laboratory (SECT Lab), Department of Chemistry, Faculty of Engineering and Technology, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu District, Tamil Nadu, India. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Chem Rec JT - Chemical record (New York, N.Y.) JID - 101085550 SB - IM OTO - NOTNLM OT - catalysis OT - gold OT - mechanochemistry OT - organometallics OT - solvent-less synthesis EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 02:04 PHST- 2026/10/12 00:00 [revised] PHST- 2026/06/13 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:04 [entrez] AID - e70266 [pii] AID - 10.1002/tcr.70266 [doi] PST - aheadofprint SO - Chem Rec. 2026 Oct 28:e70266. doi: 10.1002/tcr.70266. PMID- 42952159 OWN - NLM STAT- Publisher LR - 20261029 IS - 2198-3844 (Electronic) IS - 2198-3844 (Linking) DP - 2026 Oct 28 TI - ReLink: Reliable Deep Learning for Microscopic Motion Tracking. LID - e78541 [pii] LID - 10.1002/advs.78541 [doi] AB - Single-particle tracking (SPT) serves as a fundamental technique for deciphering complex microscopic dynamics in biological and physical systems by resolving trajectories of individual particles. However, linking localizations into trajectories remains challenging in realistic experiments, where crowding, blinking, and localization errors lead to linking ambiguity. Both statistical and deep-learning approaches have been explored in these conditions, yet reliable link selection that generalizes across varying data distributions remains elusive. Here, we propose ReLink, a general deep-learning framework for reliable trajectory linking in SPT. By integrating physical priors into geometric representations and leveraging confidence-based adaptive filtering at inference, ReLink enables automated selection of trustworthy linkages, thereby preserving the fidelity of downstream trajectory-based analyses. Across challenging simulated scenarios spanning broad experimental conditions and motion regimes, ReLink consistently outperforms representative statistical and deep learning-based trajectory linking methods in both edge-level and trajectory-level evaluation metrics, leading to markedly improved downstream inference on public benchmarks. We further validate ReLink on diverse real-world experiments, from free diffusion systems to crowded intracellular environments, demonstrating its practical significance for quantitative interpretation of microscopic dynamics and its promise for next-generation trustworthy and automated SPT. CI - (c) 2026 The Author(s). Advanced Science published by Wiley-VCH GmbH. FAU - Jiang, Yuan AU - Jiang Y AUID- ORCID: 0009-0003-7086-6416 AD - School of Computer Science and Technology, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong, China. FAU - Feng, Xiaochen AU - Feng X AUID- ORCID: 0009-0001-9582-017X AD - School of Computer Science and Technology, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong, China. FAU - Wei, Hongda AU - Wei H AD - Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School (SIGS), Tsinghua University, Shenzhen, Guangdong, China. FAU - Sha, Hao AU - Sha H AUID- ORCID: 0000-0002-3231-1570 AD - School of Computer Science and Technology, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong, China. FAU - Wang, Hongpeng AU - Wang H AUID- ORCID: 0000-0001-8108-2674 AD - School of Computer Science and Technology, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong, China. FAU - Zhang, Min AU - Zhang M AD - School of Computer Science and Technology, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong, China. FAU - Han, Sanyang AU - Han S AUID- ORCID: 0000-0001-7414-7193 AD - Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School (SIGS), Tsinghua University, Shenzhen, Guangdong, China. FAU - Zhang, Yongbing AU - Zhang Y AUID- ORCID: 0000-0003-3320-2904 AD - School of Computer Science and Technology, Harbin Institute of Technology (Shenzhen), Shenzhen, Guangdong, China. LA - eng GR - 62331011/National Natural Science Foundation of China/ GR - 2023ZT10C040/Guangdong Innovative and Entrepreneurial Research Team Program/ PT - Journal Article DEP - 20261028 PL - Germany TA - Adv Sci (Weinh) JT - Advanced science (Weinheim, Baden-Wurttemberg, Germany) JID - 101664569 SB - IM PMC - PMC13717127 OTO - NOTNLM OT - reliable deep learning OT - single particle tracking OT - stochastic processes OT - trajectory linking COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 PMCR- 2026/10/28 CRDT- 2026/10/29 02:04 PHST- 2026/10/17 00:00 [revised] PHST- 2026/04/14 00:00 [received] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:04 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e78541 [pii] AID - ADVS78541 [pii] AID - 10.1002/advs.78541 [doi] PST - aheadofprint SO - Adv Sci (Weinh). 2026 Oct 28:e78541. doi: 10.1002/advs.78541. PMID- 42952156 OWN - NLM STAT- Publisher LR - 20261029 IS - 2198-3844 (Electronic) IS - 2198-3844 (Linking) DP - 2026 Oct 28 TI - Stroke-Heart Syndrome: Mechanisms, Temporal Phenotypes, and Diagnostic Reasoning. LID - e78548 [pii] LID - 10.1002/advs.78548 [doi] AB - Stroke-heart syndrome (SHS) encompasses new or worsened cardiac abnormalities that arise after acute stroke, most often within the first 30 days and with an early peak. Its spectrum includes acute myocardial injury, electrocardiographic changes, arrhythmia, ventricular dysfunction, Takotsubo syndrome, and heart failure, but similar findings may reflect antecedent cardiac disease or systemic illness. This Review defines the accepted clinical boundaries of SHS and integrates central autonomic network disruption, neuroendocrine stress, inflammation, endothelial and microvascular dysfunction, oxygen supply-demand imbalance, and premorbid substrate into a time-resolved framework. Eight provisional, non-mutually-exclusive phenotypic domains are proposed to organize current evidence without implying validated endotypes. A diagnostic approach based on symptom chronology, prior cardiac data, serial troponin and electrocardiography, rhythm monitoring, echocardiography, selective coronary assessment, and cardiac magnetic resonance is presented to distinguish stroke-triggered injury from recent myocardial infarction, pre-existing atrial fibrillation, chronic myocardial injury, and established heart failure. No syndrome-specific treatment is validated; care should prioritize recognized cardiac emergencies, correction of reversible stressors, and coordinated stroke-cardiology decision-making. Prospective studies with documented premorbid status, standardized time points, and blinded adjudication are needed to validate the proposed phenotypes and determine whether they improve outcomes. CI - (c) 2026 The Author(s). Advanced Science published by Wiley-VCH GmbH. FAU - Lin, Fa AU - Lin F AUID- ORCID: 0000-0001-8705-2652 AD - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, People's Republic of China. FAU - Lyu, Jian AU - Lyu J AUID- ORCID: 0000-0001-6518-622X AD - Department of Neurology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China. AD - NMPA Key Laboratory for Clinical Research and Evaluation of Traditional Chinese Medicine, Beijing, People's Republic of China. AD - China National Clinical Research Center for Chinese Medicine Cardiology, Beijing, People's Republic of China. FAU - Liu, Yanci AU - Liu Y AUID- ORCID: 0000-0002-6950-0770 AD - Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing, People's Republic of China. FAU - Feng, Zhiyuan AU - Feng Z AUID- ORCID: 0000-0002-7433-2994 AD - Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China. FAU - Bao, Jie AU - Bao J AUID- ORCID: 0009-0002-7416-6786 AD - Senior Department of Cardiology, The Sixth Medical Center of PLA General Hospital, Beijing, People's Republic of China. FAU - Song, Wenxiong AU - Song W AUID- ORCID: 0000-0001-9893-5071 AD - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, People's Republic of China. FAU - Zhu, Bingcheng AU - Zhu B AUID- ORCID: 0000-0001-7868-6821 AD - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, People's Republic of China. FAU - Liu, Minghao AU - Liu M AUID- ORCID: 0009-0006-7889-5205 AD - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, People's Republic of China. FAU - Xue, Jionghao AU - Xue J AUID- ORCID: 0009-0002-6812-6630 AD - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, People's Republic of China. FAU - Weng, Jiadi AU - Weng J AUID- ORCID: 0009-0005-9236-2037 AD - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, People's Republic of China. FAU - Zhang, Jiani AU - Zhang J AUID- ORCID: 0009-0009-4468-5543 AD - Beijing Chaoyang Hospital, Capital Medical University, Beijing, People's Republic of China. FAU - Zhao, Jizong AU - Zhao J AUID- ORCID: 0000-0001-7304-0255 AD - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, People's Republic of China. FAU - Chen, Xiaolin AU - Chen X AD - Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing, People's Republic of China. AD - China National Clinical Research Center for Neurological Diseases, Beijing, People's Republic of China. FAU - Dong, Bin AU - Dong B AUID- ORCID: 0000-0003-0211-7344 AD - Affiliated Hospital of Hebei University, Baoding, Hebei, People's Republic of China. LA - eng GR - 82602508/National Natural Science Foundation of China/ GR - ZF2026436/Government-Funded Project for Outstanding Clinical Medical Talents/ GR - BJBH2026-445638/Beijing Postdoctoral Research Foundation/ PT - Journal Article PT - Review DEP - 20261028 PL - Germany TA - Adv Sci (Weinh) JT - Advanced science (Weinheim, Baden-Wurttemberg, Germany) JID - 101664569 SB - IM PMC - PMC13717121 OTO - NOTNLM OT - autonomic dysfunction OT - brain-heart axis OT - diagnostic reasoning OT - myocardial injury OT - stroke-heart syndrome COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 PMCR- 2026/10/28 CRDT- 2026/10/29 02:03 PHST- 2026/10/11 00:00 [revised] PHST- 2026/06/17 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 02:03 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e78548 [pii] AID - ADVS78548 [pii] AID - 10.1002/advs.78548 [doi] PST - aheadofprint SO - Adv Sci (Weinh). 2026 Oct 28:e78548. doi: 10.1002/advs.78548. PMID- 42952142 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1526-9914 (Electronic) IS - 1526-9914 (Linking) VI - 27 IP - 11 DP - 2026 Nov TI - Vision and implementation for the first U.S. carbon ion therapy center part I: Clinical rationale and the need for CIRT in the United States. LID - e70876 [pii] LID - 10.1002/acm2.70876 [doi] AB - Carbon ion radiotherapy (CIRT) has evolved into an established clinical modality over the past three decades. Owing to its unique physical and biological characteristics, CIRT has demonstrated favorable outcomes for selected malignancies characterized by radioresistance, proximity to critical organs, locally advanced disease requiring safe dose escalation, and recurrent tumors requiring re-irradiation. This review summarizes the physical and radiobiological rationale for CIRT, the evolution of international clinical practice and the clinical relative biological effectiveness models and dose prescription systems currently used in Asia and Europe. We discuss the differences between the modified microdosimetric kinetic model and Local Effect Model frameworks and their implications for interpreting and applying international clinical experience. We also describe the biological dose calculation framework adopted by Mayo Clinic Florida (MCF) to integrate established international CIRT experience into the first U.S. program. Published estimates of CIRT eligibility and need in the United States are reviewed to provide context for the anticipated U.S. patient population. This population includes many clinically complex disease sites characterized by proximity to critical organs, respiratory motion, anatomical variation, and the potential need for adaptive treatment. These clinical characteristics define important requirements for the first U.S. CIRT program and provide the foundation for the design philosophy and technical implementation described in the companion Part II article. CI - (c) 2026 The Author(s). Journal of Applied Clinical Medical Physics published by Wiley Periodicals, LLC on behalf of The American Association of Physicists in Medicine. FAU - Liang, Xiaoying AU - Liang X AUID- ORCID: 0000-0002-6688-1045 AD - Department of Radiation Oncology, Mayo Clinic, Jacksonville, Florida, USA. FAU - Beltran, Chris J AU - Beltran CJ AD - Department of Radiation Oncology, Mayo Clinic, Jacksonville, Florida, USA. LA - eng PT - Journal Article PT - Review PL - United States TA - J Appl Clin Med Phys JT - Journal of applied clinical medical physics JID - 101089176 SB - IM MH - Humans MH - *Heavy Ion Radiotherapy/methods MH - United States MH - *Neoplasms/radiotherapy MH - Radiotherapy Dosage MH - *Radiotherapy Planning, Computer-Assisted/methods MH - Relative Biological Effectiveness MH - *Organs at Risk/radiation effects PMC - PMC13717058 OTO - NOTNLM OT - carbon ion radiotherapy OT - clinical indications OT - united states COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/28 CRDT- 2026/10/29 01:53 PHST- 2026/09/30 00:00 [revised] PHST- 2026/07/15 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 01:53 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e70876 [pii] AID - ACM270876 [pii] AID - 10.1002/acm2.70876 [doi] PST - ppublish SO - J Appl Clin Med Phys. 2026 Nov;27(11):e70876. doi: 10.1002/acm2.70876. PMID- 42952140 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1552-5279 (Electronic) IS - 1552-5260 (Print) IS - 1552-5260 (Linking) VI - 22 IP - 10 DP - 2026 Oct TI - EEG-based brain effective and functional connectivity predict PET-defined amyloid positivity in cognitively normal individuals. LID - e71931 [pii] LID - 10.1002/alz.71931 [doi] AB - INTRODUCTION: Early identification of amyloid-beta (Abeta) pathology is essential for timely intervention in Alzheimer's disease (AD), as Abeta accumulation precedes clinical symptoms by years. METHODS: We predicted positron emission tomography (PET) -defined Abeta status in cognitively normal individuals using non-invasive resting-state dry electroencephalography (EEG). Brain effective and functional connectivity were quantified to characterize dynamic information transfer between regions. Features derived from dynamic brain connectivity were used to train machine learning models for Abeta classification. RESULTS: EEG-based models can predict amyloid positivity with accuracy comparable to, or in some cases exceeding, that of plasma p-tau217 and previously reported predictors based on cognitive measures, demographics, and health factors, depending on the model. DISCUSSION: Beyond near real-time Abeta inference, the framework identifies the most affected brain regions and connections associated with neurodegeneration, offering complementary information to blood-based biomarkers and supporting its potential as an affordable tool for early AD detection in primary care and population screening. CI - (c) 2026 The Author(s). Alzheimer's & Dementia published by Wiley Periodicals LLC on behalf of Alzheimer's Association. FAU - Sun, Boxin AU - Sun B AD - Department of Electrical and Computer Engineering, Michigan State University, East Lansing, Michigan, USA. AD - Michigan Alzheimer's Disease Research Center, Ann Arbor, Michigan, USA. FAU - Gu, Ming AU - Gu M AD - Department of Electrical and Computer Engineering, Michigan State University, East Lansing, Michigan, USA. AD - Michigan Alzheimer's Disease Research Center, Ann Arbor, Michigan, USA. FAU - Maher, Amanda AU - Maher A AD - Michigan Alzheimer's Disease Research Center, Ann Arbor, Michigan, USA. AD - Department of Psychiatry, University of Michigan, Ann Arbor, Michigan, USA. FAU - DuBois, Kelly N AU - DuBois KN AD - Michigan Alzheimer's Disease Research Center, Ann Arbor, Michigan, USA. AD - Department of Translational Neuroscience, Michigan State University, Grand Rapids, Michigan, USA. FAU - Kanaan, Nicholas M AU - Kanaan NM AD - Michigan Alzheimer's Disease Research Center, Ann Arbor, Michigan, USA. AD - Department of Translational Neuroscience, Michigan State University, Grand Rapids, Michigan, USA. FAU - Bross, Jordan AU - Bross J AD - Michigan Alzheimer's Disease Research Center, Ann Arbor, Michigan, USA. AD - Department of Psychiatry, University of Michigan, Ann Arbor, Michigan, USA. FAU - Koeppe, Robert AU - Koeppe R AD - Michigan Alzheimer's Disease Research Center, Ann Arbor, Michigan, USA. AD - Department of Radiology, University of Michigan, Ann Arbor, Michigan, USA. FAU - Persad, Carol C AU - Persad CC AD - Michigan Alzheimer's Disease Research Center, Ann Arbor, Michigan, USA. AD - Department of Psychiatry, University of Michigan, Ann Arbor, Michigan, USA. FAU - Murphey, Yi L AU - Murphey YL AD - Department of Electrical and Computer Engineering, University of Michigan-Dearborn, Dearborn, Michigan, USA. FAU - Kavcic, Voyko AU - Kavcic V AD - Michigan Alzheimer's Disease Research Center, Ann Arbor, Michigan, USA. AD - Institute of Gerontology, Wayne State University, Detroit, Michigan, USA. AD - International Institute of Applied Gerontology, Ljubljana, Slovenia. FAU - Ren, Jian AU - Ren J AD - Department of Electrical and Computer Engineering, Michigan State University, East Lansing, Michigan, USA. FAU - Giordani, Bruno AU - Giordani B AD - Michigan Alzheimer's Disease Research Center, Ann Arbor, Michigan, USA. AD - Department of Psychiatry, University of Michigan, Ann Arbor, Michigan, USA. FAU - Li, Tongtong AU - Li T AD - Department of Electrical and Computer Engineering, Michigan State University, East Lansing, Michigan, USA. AD - Michigan Alzheimer's Disease Research Center, Ann Arbor, Michigan, USA. LA - eng GR - NSF-2032709/National Science Foundation/ GR - NSF-1919154/National Science Foundation/ GR - NSF-2535060/National Science Foundation/ GR - P30AG072931/NH/NIH HHS/United States GR - P30AG024824/NH/NIH HHS/United States GR - S10OD034250-01/NH/NIH HHS/United States GR - R01AG068338/NH/NIH HHS/United States PT - Journal Article PL - United States TA - Alzheimers Dement JT - Alzheimer's & dementia : the journal of the Alzheimer's Association JID - 101231978 RN - 0 (Amyloid beta-Peptides) RN - 0 (tau Proteins) RN - 0 (Biomarkers) SB - IM MH - Humans MH - *Positron-Emission Tomography MH - *Brain/diagnostic imaging/physiology/metabolism MH - *Electroencephalography/methods MH - Female MH - *Amyloid beta-Peptides/metabolism MH - Male MH - Alzheimer Disease/diagnosis MH - tau Proteins/blood MH - Aged MH - Machine Learning MH - Cognition/physiology MH - Biomarkers PMC - PMC13717061 OTO - NOTNLM OT - EEG OT - amyloid-beta OT - brain effective and functional connectivity OT - early screening of Alzheimer's disease OT - p-Tau217 COIS- The authors declare no conflict of interest. Author disclosures are available in the supporting information. Author disclosures are available in the Supporting Information. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/28 CRDT- 2026/10/29 01:53 PHST- 2026/08/16 00:00 [revised] PHST- 2026/04/21 00:00 [received] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 01:53 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e71931 [pii] AID - ALZ71931 [pii] AID - 10.1002/alz.71931 [doi] PST - ppublish SO - Alzheimers Dement. 2026 Oct;22(10):e71931. doi: 10.1002/alz.71931. PMID- 42952137 OWN - NLM STAT- Publisher LR - 20261029 IS - 1097-0029 (Electronic) IS - 1059-910X (Linking) DP - 2026 Oct 28 TI - DINOSim: Training-Free Semantic Segmentation on Microscopy Images. LID - 10.1002/jemt.70194 [doi] AB - We present DINOSim, a training-free method for segmenting objects in microscopy images without the need for large annotated datasets or additional model training. DINOSim builds on the pretrained DINOv2 image encoder, which captures semantic information from images. By comparing the encoder's features of image patches to those of a user-selected reference, DINOSim generates pseudo-labels that guide semantic segmentation. Subsequently, a k-nearest neighbors framework is used to refine predictions across new images. Our experiments show that DINOSim can effectively segment previously unseen objects in diverse microscopy datasets, offering performance comparable to supervised approaches while avoiding the need for costly manual labeling. We also investigate how different choices of user prompt selection and model size affect accuracy and generalization. To make the method widely accessible, we provide an open-source napari plugin (github.com/AAitorG/napari-DINOSim), enabling researchers to apply DINOSim to their own data easily. Overall, DINOSim offers a fast, flexible and practical solution for bioimage analysis, particularly valuable in resource-constrained settings. CI - (c) 2026 The Author(s). Microscopy Research and Technique published by Wiley Periodicals LLC. FAU - Gonzalez-Marfil, Aitor AU - Gonzalez-Marfil A AUID- ORCID: 0000-0003-1367-4154 AD - Department of Computer Science and Artificial Intelligence, University of the Basque Country (UPV/EHU), San Sebastian, Spain. AD - Donostia International Physics Center (DIPC), San Sebastian, Spain. FAU - Gomez-de-Mariscal, Estibaliz AU - Gomez-de-Mariscal E AUID- ORCID: 0000-0003-2082-3277 AD - Antonio Xavier Institute of Chemical and Biological Technology, Universidade Nova de Lisboa, Oeiras, Portugal. AD - NOVA Institute for Medical Systems Biology, Association, NOVA IMSB, Paco de Arcos, Portugal. AD - iNOVA4Health, Programme in Translational Medicine, Lisboa, Portugal. FAU - Arganda-Carreras, Ignacio AU - Arganda-Carreras I AUID- ORCID: 0000-0003-0229-5722 AD - Department of Computer Science and Artificial Intelligence, University of the Basque Country (UPV/EHU), San Sebastian, Spain. AD - Donostia International Physics Center (DIPC), San Sebastian, Spain. AD - Biofisika Institute (CSIC-UPV/EHU), Leioa, Spain. AD - IKERBASQUE, Basque Foundation for Science, Bilbao, Spain. LA - eng GR - PID2024-157485NB-I00/Ministerio de Ciencia, Innovacion y Universidades/ GR - RED2024-153844-T/Ministerio de Ciencia, Innovacion y Universidades/ GR - AIA2025-164165-C42/Ministerio de Ciencia, Innovacion y Universidades/ PT - Journal Article DEP - 20261028 PL - United States TA - Microsc Res Tech JT - Microscopy research and technique JID - 9203012 SB - IM OTO - NOTNLM OT - foundation models OT - image segmentation OT - training-free EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 01:52 PHST- 2026/09/15 00:00 [revised] PHST- 2026/07/14 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 01:52 [entrez] AID - 10.1002/jemt.70194 [doi] PST - aheadofprint SO - Microsc Res Tech. 2026 Oct 28. doi: 10.1002/jemt.70194. PMID- 42952136 OWN - NLM STAT- Publisher LR - 20261029 IS - 1521-4095 (Electronic) IS - 0935-9648 (Linking) DP - 2026 Oct 28 TI - Modulating Local Electronegativity in Covalent Organic Frameworks for Host-Guest Oxygen Reduction. LID - e75544 [pii] LID - 10.1002/adma.75544 [doi] AB - Developing high-performance metal-free electrocatalysts for the oxygen reduction reaction remains a major challenge in sustainable energy conversion. Herein, we report a host-guest chemistry strategy based on metal-free covalent organic frameworks (COFs) to significantly enhance the catalytic performance. Unlike conventional COFs that focus primarily on linkage chemistry or heteroatom doping, our approach explicitly leverages molecular-level design to create tailored binding pockets within the COFs pores, enabling high selectivity and strong interactions with O(2). A series of fluorene-based COF hosts with systematically tuned electronegativity are designed and synthesized to strengthen molecular recognition and binding toward oxygen via tailored electronic and structural environments. The optimized material achieves a remarkable half-wave potential (E(1/2), vs. RHE) of 0.78 V, among the highest reported metal-free COF-based catalysts. This exceptional performance originates from enhanced host-guest recognition, which effectively facilitates O(2) adsorption while optimizing the stabilization and subsequent dissociation of key reaction intermediates on the active site. Through a combination of in situ characterization and theoretical calculations, these host-guest interactions promote the optimized adsorption of intermediates and accelerate reaction kinetics. This study provides a new paradigm for the design of high-efficiency metal-free electrocatalysts through precise molecular recognition, opening avenues for advanced catalytic systems beyond conventional approaches. CI - (c) 2026 Wiley-VCH GmbH. FAU - Zhao, Genfu AU - Zhao G AUID- ORCID: 0009-0004-8591-2785 AD - National Center for International Joint Research of Photoelectric Energy Materials and Application, International Joint Research Center for Advanced Energy Materials of Yunnan Province, School of Materials and Energy, Yunnan University, Kunming, China. FAU - Zou, Xiaoxiao AU - Zou X AD - National Center for International Joint Research of Photoelectric Energy Materials and Application, International Joint Research Center for Advanced Energy Materials of Yunnan Province, School of Materials and Energy, Yunnan University, Kunming, China. FAU - Cai, Lebin AU - Cai L AD - State Key Laboratory of New Textile Materials and Advanced Processing, Key Laboratory of Material Chemistry for Energy Conversion and Storage (Ministry of Education), Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), Wuhan, China. FAU - Gong, Zhemin AU - Gong Z AD - National Center for International Joint Research of Photoelectric Energy Materials and Application, International Joint Research Center for Advanced Energy Materials of Yunnan Province, School of Materials and Energy, Yunnan University, Kunming, China. FAU - Sun, Yongjiang AU - Sun Y AD - National Center for International Joint Research of Photoelectric Energy Materials and Application, International Joint Research Center for Advanced Energy Materials of Yunnan Province, School of Materials and Energy, Yunnan University, Kunming, China. FAU - Qi, Kai AU - Qi K AUID- ORCID: 0000-0002-3641-1165 AD - State Key Laboratory of New Textile Materials and Advanced Processing, Key Laboratory of Material Chemistry for Energy Conversion and Storage (Ministry of Education), Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), Wuhan, China. FAU - Xia, Bao Yu AU - Xia BY AUID- ORCID: 0000-0002-2054-908X AD - State Key Laboratory of New Textile Materials and Advanced Processing, Key Laboratory of Material Chemistry for Energy Conversion and Storage (Ministry of Education), Hubei Key Laboratory of Material Chemistry and Service Failure, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology (HUST), Wuhan, China. AD - Center for Next-Generation Energy Materials, School of Chemical Engineering, Sungkyunkwan University (SKKU), Suwon, Gyeonggi-do, Republic of Korea. FAU - Guo, Hong AU - Guo H AUID- ORCID: 0000-0001-5693-2980 AD - National Center for International Joint Research of Photoelectric Energy Materials and Application, International Joint Research Center for Advanced Energy Materials of Yunnan Province, School of Materials and Energy, Yunnan University, Kunming, China. LA - eng GR - 22561160130/NSFC-Research Grants Council of Hong Kong Joint Research Scheme/ GR - U25D9010/Major Program of Joint Funds of NSFC/ GR - W2441008/Research Plan of International Collaboration Fund for Creative Research Teams (ICFCRT) of NSFC/ GR - 202301AS070040/Natural Science Foundation of Yunnan Province/ PT - Journal Article DEP - 20261028 PL - Germany TA - Adv Mater JT - Advanced materials (Deerfield Beach, Fla.) JID - 9885358 SB - IM OTO - NOTNLM OT - covalent organic frameworks OT - electronegativity OT - host-guest chemistry OT - oxygen reduction EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 01:52 PHST- 2026/10/15 00:00 [revised] PHST- 2026/07/31 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 01:52 [entrez] AID - e75544 [pii] AID - 10.1002/adma.75544 [doi] PST - aheadofprint SO - Adv Mater. 2026 Oct 28:e75544. doi: 10.1002/adma.75544. PMID- 42952135 OWN - NLM STAT- Publisher LR - 20261029 IS - 1941-0921 (Electronic) IS - 1941-0921 (Linking) DP - 2026 Oct 28 TI - Sleep Strategies and Performance Outcomes in Ultramarathon Runners: A Systematic Review. PG - 19417381261494498 LID - 10.1177/19417381261494498 [doi] AB - CONTEXT: Ultramarathon events impose prolonged physiological and cognitive demands, often spanning 1 or more nights with limited sleep opportunity. OBJECTIVE: To examine associations between pre-race and intra-race sleep strategies and physical and cognitive performance outcomes in ultramarathon runners. DATA SOURCES: PubMed, EMBASE, PsycINFO, SPORTDiscus, and Web of Science were searched from inception to July 25, 2025. STUDY SELECTION: Primary observational or experimental studies published in English, or available in English translation were eligible when they involved ultramarathon runners (>42.2 km) and reported sleep as a variable of interest in relation to race performance or cognitive performance. STUDY DESIGN: Systematic review. LEVEL OF EVIDENCE: Level 3. DATA EXTRACTION: Two reviewers independently extracted study design, participant characteristics, race characteristics, sleep outcomes, performance outcomes, and cognitive outcomes. Methodological quality was assessed using the Joanna Briggs Institute critical appraisal checklists. RESULTS: Nine studies involving 2262 participants were included, with overall low risk of bias. Of the 4 studies examining pre-race sleep, 3 reported a significant association or group difference with a race-performance outcome. Reduced sleep was generally associated with poorer physical and cognitive performance. Greater intra-race sleep duration was consistently associated with longer finish times, reflecting additional nonmoving time and increased sleep need in longer events. Greater pre-race sleep duration, including sleep extension, was associated with faster finish times, and better pre-race sleep quality was linked to higher cognitive performance. CONCLUSION: Pre-race sleep extension was associated with faster finish times, although the observational evidence does not establish causality. Further research using objective sleep measures and controlled designs is required. FAU - Brown, Daniel A AU - Brown DA AUID- ORCID: 0000-0002-1077-4021 AD - Physiotherapy Department, Gold Coast Hospital and Health Service, Gold Coast, Queensland, Australia. AD - Physiotherapy Department, Bond University, Gold Coast, Queensland, Australia. AD - Griffith University, School of Allied Health, Sport and Social Work, Parklands Drive, Southport Gold Coast Queensland, Australia. FAU - Parreira, Jason AU - Parreira J AD - Doctor of Physiotherapy Student, Bond University, Gold Coast, Queensland, Australia. FAU - Spencer, Katie AU - Spencer K AD - Doctor of Physiotherapy Student, Bond University, Gold Coast, Queensland, Australia. FAU - Skinner, Serena AU - Skinner S AD - Doctor of Physiotherapy Student, Bond University, Gold Coast, Queensland, Australia. FAU - Goto, Wataru AU - Goto W AD - Physiotherapy Department, Gold Coast Hospital and Health Service, Gold Coast, Queensland, Australia. FAU - Sattler, Larissa AU - Sattler L AUID- ORCID: 0000-0001-6023-8182 AD - Physiotherapy Department, Gold Coast Hospital and Health Service, Gold Coast, Queensland, Australia. AD - Physiotherapy Department, Bond University, Gold Coast, Queensland, Australia. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Sports Health JT - Sports health JID - 101518422 SB - IM OTO - NOTNLM OT - cognitive performance OT - endurance OT - intra-race sleep OT - sleep deprivation OT - sleep extension EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 01:52 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 01:52 [entrez] AID - 10.1177/19417381261494498 [doi] PST - aheadofprint SO - Sports Health. 2026 Oct 28:19417381261494498. doi: 10.1177/19417381261494498. PMID- 42952127 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1478-5153 (Electronic) IS - 1362-1017 (Linking) VI - 31 IP - 6 DP - 2026 Nov TI - QUIET Care: A Nursing-Led Conceptual Analysis of Alarm-Related Acoustic Burden in Adult Critical Care. LID - e70745 [pii] LID - 10.1111/nicc.70745 [doi] AB - BACKGROUND: Adult intensive care units (ICUs) generate a persistent acoustic burden from alarms, care activity, equipment and communication. This burden can undermine patient rest and contribute to alarm fatigue, cognitive workload and distress among nurses. AIM: To present a nursing-led conceptual framework for addressing alarm-related acoustic burden in adult critical care. STUDY DESIGN: Literature-informed conceptual analysis. A structured purposive evidence map was audited using two PubMed searches run on 9 September 2026 (3377 unique records after PMID deduplication) and citation checking. Twenty-nine selected sources were charted against the five domains. This was not a systematic review; no original data, formal consensus or framework validation was undertaken. RESULTS: QUIET Care proposes five connected practice domains: quantify and qualify the sound environment; use patient-specific monitoring and alarms; integrate quiet into workflow; engage people and culture; and track, test and learn. It treats the domains as locally adaptable hypotheses and requires explicit safety balancing measures. CONCLUSIONS: QUIET Care is a pragmatic proposal for local co-design and evaluation, not a validated intervention or universal sound target. RELEVANCE TO CLINICAL PRACTICE: The framework may help critical care nurses and leaders organise source mapping, alarm customisation, workflow redesign, team ownership and small safety-monitored tests of change while preserving the audibility of urgent alerts. CI - (c) 2026 British Association of Critical Care Nurses. FAU - Alsharari, Abdalkarem Fedgash AU - Alsharari AF AUID- ORCID: 0000-0002-8911-3356 AD - College of Nursing, Jouf University, Sakaka, Saudi Arabia. FAU - Shaban, Mostafa AU - Shaban M AUID- ORCID: 0000-0001-7822-1653 AD - College of Nursing, Jouf University, Sakaka, Saudi Arabia. LA - eng GR - DGSSR-2023-01-02549/Deanship of Graduate Studies and Scientific Research at Jouf University/ PT - Journal Article PT - Review PL - England TA - Nurs Crit Care JT - Nursing in critical care JID - 9808649 SB - IM MH - Humans MH - *Clinical Alarms/adverse effects MH - *Noise/adverse effects MH - *Intensive Care Units MH - *Critical Care MH - *Critical Care Nursing MH - Working Conditions MH - Adult OTO - NOTNLM OT - clinical alarms OT - critical care nursing OT - intensive care units OT - noise OT - patient safety EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 CRDT- 2026/10/29 01:43 PHST- 2026/09/11 00:00 [revised] PHST- 2026/08/13 00:00 [received] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 01:43 [entrez] AID - e70745 [pii] AID - 10.1111/nicc.70745 [doi] PST - ppublish SO - Nurs Crit Care. 2026 Nov;31(6):e70745. doi: 10.1111/nicc.70745. PMID- 42952126 OWN - NLM STAT- Publisher LR - 20261029 IS - 1941-0921 (Electronic) IS - 1941-0921 (Linking) DP - 2026 Oct 28 TI - Efficacy of Apparent Diffusion Coefficient Sampling in Muscle Tears and Intersex Differences in Professional Football Players: A Cohort Study. PG - 19417381261492942 LID - 10.1177/19417381261492942 [doi] AB - BACKGROUND: Magnetic resonance imaging (MRI) is the gold standard for monitoring the evolution of muscle injuries in athletes, although no established quantitative measure of recovery currently exists. HYPOTHESIS: Apparent diffusion coefficient (ADC) values may provide a quantitative measure of muscle healing, and sex-related differences may exist in the evolution of muscle injuries. STUDY DESIGN: Single-center retrospective observational cohort study. LEVEL OF EVIDENCE: Level 2a. METHODS: Twenty-six professional football (soccer) players (13 male ,13 female) undergoing MRI for muscle injury were retrospectively selected between October 2020 and June 2024. All athletes underwent MRI at baseline (T0) and at 2-week follow-up, including diffusion-weighted imaging (DWI) acquisitions with b values of 0-100-800 s/mm((2)). Two musculoskeletal radiologists measured ADC values within the index lesions at each examination. Changes in ADC between the two time points (DeltaADC) were assessed using a paired-samples t test, while sex-related differences were evaluated using the Mann-Whitney U test. RESULTS: The overall mean ADC value at the lesion site decreased from 2.06 +/- 0.35 mm(2)/s at T0 to 1.61 +/- 0.30 mm(2)/s at follow-up corresponding to a mean DeltaADC of 0.44 +/- 0.20 and a 21.84% reduction. The difference between T0 and follow-up was statistically significant (t = 8.12; P < 0.001). Mean DeltaADC values were 0.32 +/- 0.20 mm((2))/s in male players and 0.56 +/- 0.25 mm((2))/s in female players, respectively, with a statistically significant difference between sexes (z = -2.95; P = 0.003). When only hamstring injuries were considered, female players showed a significantly greater decrease in ADC over time than male players (Mann-Whitney U test; exact P = 0.02), with mean DeltaADC values of 0.56 +/- 0.30 and 0.26 +/- 0.08 mm((2))/s, respectively. CONCLUSION: Muscle injury is associated with an acute increase in ADC values followed by a progressive decrease during healing. ADC may therefore represent a quantitative imaging biomarker for monitoring muscle recovery. CLINICAL RELEVANCE: ADC measurement within muscle tears may provide a quantitative assessment of healing status and support individualized rehabilitation strategies in athletes. Sex-related differences may exist in injury pathophysiology and recovery patterns relevant to safe return to play. FAU - Lusi, Stefano AU - Lusi S AUID- ORCID: 0000-0001-9650-493X AD - Department of Diagnostic and Interventional Radiology, IRCCS Humanitas Research Hospital, Milan, Italy, and Department of Musculoskeletal Radiology, Hopital, AP-HP Centre, Paris, France. FAU - Carnicelli, Giorgia AU - Carnicelli G AUID- ORCID: 0000-0001-8738-5561 AD - Department of Diagnostic and Interventional Radiology, IRCCS Humanitas Research Hospital, Milan, Italy, and Department of Biomedical Sciences, Humanitas University, Milan, Italy. FAU - Quaglia, Alessandro AU - Quaglia A AD - Department of Knee Orthopaedic and Sports Traumatology Unit, Humanitas Research Hospital, Rozzano, Milan, Italy, and FC Internazionale Medical Staff, Milan, Italy. FAU - Carimati, Giulia AU - Carimati G AD - Department of Knee Orthopaedic and Sports Traumatology Unit, Humanitas Research Hospital, Rozzano, Milan, Italy, and FC Internazionale Medical Staff, Milan, Italy. FAU - Volpi, Piero AU - Volpi P AD - Department of Knee Orthopaedic and Sports Traumatology Unit, Humanitas Research Hospital, Rozzano, Milan, Italy, and FC Internazionale Medical Staff, Milan, Italy. FAU - Francone, Marco AU - Francone M AD - Department of Diagnostic and Interventional Radiology, IRCCS Humanitas Research Hospital, Milan, Italy, and Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Milan, Italy. FAU - Balzarini, Luca AU - Balzarini L AD - Department of Diagnostic and Interventional Radiology, IRCCS Humanitas Research Hospital, Milan, Italy. FAU - Magarelli, Nicola AU - Magarelli N AD - Department of Diagnostic and Interventional Radiology, IRCCS Humanitas Research Hospital, Milan, Italy, and Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Milan, Italy. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Sports Health JT - Sports health JID - 101518422 SB - IM OTO - NOTNLM OT - apparent diffusion coefficient OT - athlete OT - muscle tear OT - sports injury EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 01:43 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 01:43 [entrez] AID - 10.1177/19417381261492942 [doi] PST - aheadofprint SO - Sports Health. 2026 Oct 28:19417381261492942. doi: 10.1177/19417381261492942. PMID- 42952125 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1521-2254 (Electronic) IS - 1099-498X (Print) IS - 1099-498X (Linking) VI - 28 IP - 11 DP - 2026 Nov TI - Pan-Cancer Landscape of TMEM9 With Experimental Validation in Head and Neck Squamous Cell Carcinoma. LID - e70117 [pii] LID - 10.1002/jgm.70117 [doi] AB - BACKGROUND: Transmembrane protein 9 (TMEM9) has been implicated in tumor progression, but its pan-cancer diagnostic, prognostic, and immunological relevance remains incompletely characterized. METHODS: TMEM9 expression, diagnostic and prognostic value, genetic alterations, functional states, and immune associations were systematically evaluated using TCGA, GTEx, and complementary public cancer databases. Experimental validation was primarily performed in HNSC models using Western blotting, colony-formation, wound-healing, Transwell, and xenograft assays, with additional exploratory validation in RCC models. RESULTS: TMEM9 showed good-to-high diagnostic performance in 15 evaluable cancer cohorts, with particularly high AUCs in CHOL (1.000), KICH (0.970), LIHC (0.949), and THYM (0.925), whereas BRCA also showed strong discriminatory performance (AUC = 0.890). Higher TMEM9 expression was associated with unfavorable survival in several cancers, although its prognostic relevance varied by tumor type. TMEM9 expression was also associated with cancer-related pathways, functional states, immune cell infiltration, and immunoregulatory gene expression in a context-dependent manner. Experimentally, TMEM9 knockdown suppressed colony formation, wound closure, migration, and invasion in HNSC cells and inhibited FaDu xenograft growth. Similar inhibitory effects were additionally observed in exploratory RCC models. CONCLUSION: TMEM9 exhibits cancer-type-dependent diagnostic, prognostic, and immunological associations, whereas functional experiments support a tumor-promoting role for TMEM9 in HNSC. Exploratory RCC experiments suggest that this effect may also occur in selected renal cancer cellular contexts. CI - (c) 2026 The Author(s). The Journal of Gene Medicine published by John Wiley & Sons Ltd. FAU - Wang, Mi AU - Wang M AD - Department of Emergency, Wenzhou TCM Hospital of Zhejiang Chinese Medical University, Wenzhou, Zhejiang, China. FAU - Ying, Shangyan AU - Ying S AD - Internal Medicine of Traditional Chinese Medicine, Wenzhou TCM Hospital of Zhejiang Chinese Medical University, Wenzhou, Zhejiang, China. FAU - Weng, Chunyan AU - Weng C AD - Department of Gastroenterology, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, Zhejiang, China. FAU - Li, Youfeng AU - Li Y AUID- ORCID: 0009-0001-5918-9843 AD - Department of Rehabilitation, Wenzhou TCM Hospital of Zhejiang Chinese Medical University, Wenzhou, Zhejiang, China. LA - eng PT - Journal Article PL - England TA - J Gene Med JT - The journal of gene medicine JID - 9815764 RN - 0 (Membrane Proteins) RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - *Membrane Proteins/genetics/metabolism MH - Animals MH - Prognosis MH - *Biomarkers, Tumor/genetics MH - Gene Expression Regulation, Neoplastic MH - *Squamous Cell Carcinoma of Head and Neck/genetics/metabolism/pathology MH - Mice MH - *Head and Neck Neoplasms/genetics/metabolism/pathology MH - Cell Line, Tumor MH - Female MH - Cell Movement PMC - PMC13717056 OTO - NOTNLM OT - TMEM9 OT - diagnostic and prognostic biomarkers OT - head and neck squamous cell carcinoma OT - pan-cancer analysis OT - renal cell carcinoma OT - tumor immune microenvironment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/29 CRDT- 2026/10/29 01:42 PHST- 2026/08/31 00:00 [revised] PHST- 2026/02/20 00:00 [received] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 01:42 [entrez] PHST- 2026/10/29 00:00 [pmc-release] AID - e70117 [pii] AID - JGM70117 [pii] AID - 10.1002/jgm.70117 [doi] PST - ppublish SO - J Gene Med. 2026 Nov;28(11):e70117. doi: 10.1002/jgm.70117. PMID- 42952101 OWN - NLM STAT- Publisher LR - 20261029 IS - 1349-7006 (Electronic) IS - 1347-9032 (Print) IS - 1347-9032 (Linking) DP - 2026 Oct 28 TI - Dietary Leucine Restriction Suppresses Platelet-Driven HCC Growth by Attenuating mTOR Signaling. LID - 10.1111/cas.70574 [doi] AB - Platelets play a crucial role in regulating tumor progression, but the mechanisms underlying platelet-induced metabolic reprogramming in hepatocellular carcinoma (HCC) remain incompletely understood. Here, metabolomic profiling identified significant accumulation of leucine in HCC cells following platelet incubation. Co-culture with platelets promoted HCC cell proliferation, migration, and invasion in complete medium, whereas leucine deprivation in the co-culture medium attenuated these malignant behaviors. Mechanistically, platelets selectively stimulated the phosphorylation of S6K1 and downstream S6, an activation that was suppressed under leucine-deficient conditions. In addition, pharmacological inhibition of S6K1 activity using PF-4708671 reduced platelet-promoted cellular proliferation, migration, and invasion, confirming the S6K1/S6 axis as an essential functional effector of this response. In vivo, dietary leucine restriction reduced tumor growth following platelet pre-incubation in the MHCC-97H xenograft model. Together, these findings demonstrate that leucine accumulation and subsequent S6K1/S6 activation mediate platelet-promoted HCC progression, indicating that targeting leucine availability or S6K1 signaling may offer therapeutic strategies for platelet-associated HCC. CI - (c) 2026 The Author(s). Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. FAU - Ruan, Xeulian AU - Ruan X AD - Department of Clinical Laboratory, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China. FAU - Ou, Guiyong AU - Ou G AD - Department of Blood Transfusion, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China. FAU - Mo, Cuiju AU - Mo C AD - Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University; Key Laboratory of Clinical Laboratory Medicine of Guangxi Department of Education, Nanning, Guangxi, China. FAU - Zhang, Yi AU - Zhang Y AD - Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University; Key Laboratory of Clinical Laboratory Medicine of Guangxi Department of Education, Nanning, Guangxi, China. FAU - Qin, Shanzi AU - Qin S AD - Department of Clinical Laboratory, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China. FAU - Chen, Yongling AU - Chen Y AD - Department of Blood Transfusion, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, People's Republic of China. FAU - Li, Dan AU - Li D AD - Department of Clinical Laboratory, The First Affiliated Hospital of Guangxi Medical University; Key Laboratory of Clinical Laboratory Medicine of Guangxi Department of Education, Nanning, Guangxi, China. FAU - Nie, Liuying AU - Nie L AD - Department of Blood Transfusion, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China. FAU - Xie, Li AU - Xie L AD - Department of Clinical Laboratory, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China. FAU - Hu, Zuojian AU - Hu Z AUID- ORCID: 0000-0003-4695-1189 AD - Department of Clinical Laboratory, The Second Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Cancer Sci JT - Cancer science JID - 101168776 SB - IM PMC - PMC13717124 OTO - NOTNLM OT - S6K1 OT - hepatocellular carcinoma OT - leucine deprivation OT - metabolomics OT - platelet COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 PMCR- 2026/10/28 CRDT- 2026/10/29 01:22 PHST- 2026/10/13 00:00 [revised] PHST- 2026/05/12 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 01:22 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - CAS70574 [pii] AID - 10.1111/cas.70574 [doi] PST - aheadofprint SO - Cancer Sci. 2026 Oct 28:10.1111/cas.70574. doi: 10.1111/cas.70574. PMID- 42952099 OWN - NLM STAT- Publisher LR - 20261029 IS - 1544-0591 (Electronic) IS - 0022-0345 (Linking) DP - 2026 Oct 28 TI - Effectiveness of Community Water Fluoridation in Queensland: A Population-Based Causal Analysis Following the 2008 Policy Change. PG - 220345261491339 LID - 10.1177/00220345261491339 [doi] AB - Water fluoridation (WF) is an important preventive program against dental caries. Recent Cochrane reviews reported only limited new scientific evidence of WF. This research aimed to provide contemporary evidence on the causal relationship between WF and oral health. A causal framework was used to ensure methodological rigour in repeated cross-sectional, population-based studies of randomly selected 5 to 14 y old children in Queensland, thereby enabling controlled difference-in-differences (DiD) analyses. The primary exposure was access to WF, defined as newly fluoridated (New-WF; areas that became fluoridated since 2010), never fluoridated (No-WF), or long-term fluoridated areas (Long-WF). The outcomes were dental caries experience (decayed, missing, or filled tooth surfaces) and prevalence in the primary and permanent dentition, collected by dental examiners who were uniformly trained and calibrated during both phases. The DiD estimands were the differences in changes in outcomes between New-WF and the control (No-WF), estimated from multivariable regression models. Data of 5,407 children during 2010-2012 and 7,718 children during 2022-2024 were collected. Variations in key confounders (area and individual socioeconomic factors) were controlled for using inverse probability of treatment weights. Dental caries among children in New-WF had consistently declined relative to the control groups. In the primary dentition, the New-WF children had a 1.40-fold (95% confidence interval [CI]: 1.08 to 1.80) reduction in caries experience and a 1.28-fold (1.00 to 1.67) reduction in caries prevalence, equivalent to 1.1 fewer affected tooth surfaces and a 10.8% lower prevalence. In the permanent dentition, reductions were smaller: a 1.32-fold (0.95 to 1.84) reduction in caries experience and a 1.04-fold (0.81 to 1.33) reduction in prevalence. These findings show that WF is effective in preventing dental caries in children when disease levels are low and other preventive programs are available. This controlled DiD study provides robust contemporary scientific evidence of the effects of WF on dental caries, strengthening WF as a public health policy. FAU - Do, L G AU - Do LG AUID- ORCID: 0000-0003-3684-9949 AD - School of Dentistry, Faculty of Health, Medicine and Behavioural Sciences, The University of Queensland, Australia. FAU - Spencer, A John AU - Spencer AJ AD - Adelaide Dental School, The University of Adelaide, Australia. FAU - Roberts-Thomson, K AU - Roberts-Thomson K AD - Adelaide Dental School, The University of Adelaide, Australia. FAU - Lalloo, R AU - Lalloo R AUID- ORCID: 0000-0001-5822-1269 AD - School of Dentistry, Faculty of Health, Medicine and Behavioural Sciences, The University of Queensland, Australia. FAU - Broadbent, J AU - Broadbent J AUID- ORCID: 0000-0002-8905-398X AD - Faculty of Dentistry, University of Otago, Dunedin, New Zealand. FAU - Foley, M AU - Foley M AD - School of Dentistry, Faculty of Health, Medicine and Behavioural Sciences, The University of Queensland, Australia. FAU - Whelton, H AU - Whelton H AUID- ORCID: 0000-0002-0881-360X AD - College of Medicine and Health, University College Cork, Cork, Ireland. FAU - Le, T AU - Le T AD - School of Dentistry, Faculty of Health, Medicine and Behavioural Sciences, The University of Queensland, Australia. FAU - Herzig, M AU - Herzig M AD - Queensland Health, Brisbane, Australia. FAU - Sexton, C T AU - Sexton CT AD - School of Dentistry, Faculty of Health, Medicine and Behavioural Sciences, The University of Queensland, Australia. FAU - Gielis, E AU - Gielis E AD - Queensland Health, Brisbane, Australia. FAU - Appleyard, B AU - Appleyard B AD - Queensland Health, Brisbane, Australia. FAU - Schuch, H S AU - Schuch HS AD - School of Dentistry, Faculty of Health, Medicine and Behavioural Sciences, The University of Queensland, Australia. FAU - Ha, D H AU - Ha DH AUID- ORCID: 0009-0005-9472-7650 AD - School of Dentistry, Faculty of Health, Medicine and Behavioural Sciences, The University of Queensland, Australia. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Dent Res JT - Journal of dental research JID - 0354343 SB - IM OTO - NOTNLM OT - Australia OT - causal inference approach OT - child dental caries OT - fluoride use OT - observational study OT - population programs EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 01:13 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 01:13 [entrez] AID - 10.1177/00220345261491339 [doi] PST - aheadofprint SO - J Dent Res. 2026 Oct 28:220345261491339. doi: 10.1177/00220345261491339. PMID- 42952081 OWN - NLM STAT- Publisher LR - 20261029 IS - 2576-6422 (Electronic) IS - 2576-6422 (Linking) DP - 2026 Oct 28 TI - MXene-Protein Corona Interfaces for Molecular Profiling of Alzheimer's Disease. LID - 10.1021/acsabm.6c01228 [doi] AB - The protein corona (PC) that forms on the surface of nanomaterials upon contact with biological fluids provides a molecular snapshot of the host's physiological and pathological state. Here, we investigate two-dimensional (2D) titanium carbide (Ti3C2Tx) MXene nanosheets as nanobiointerfaces for capturing Alzheimer's disease (AD)-associated plasma protein signatures. Ti3C2Tx MXene flakes were incubated with plasma from clinically diagnosed AD patients and age-matched healthy controls (HC), leading to the formation of Ti3C2Tx MXene-PC complexes. Physicochemical characterization using dynamic light scattering, zeta potential analysis, and transmission electron microscopy revealed disease-dependent changes in hydrodynamic size, surface charge, and PC profile. Proteomic analysis of the isolated PC layers quantified 1,611 proteins without prior fractionation, demonstrating broad plasma proteome coverage. Principal component analysis (PCA) revealed disease-associated separation between AD and HC-derived Ti3C2Tx MXene PC proteomes along specific principal components despite interindividual heterogeneity. Differential abundance analysis identified selective enrichment of heterogeneous nuclear ribonucleoproteins (hnRNPs), annexins, and inflammatory mediators in AD-derived PC, implicating dysregulated RNA metabolism, membrane stress responses, and immune activation, hallmark processes in AD pathology. Our findings demonstrate that Ti3C2Tx MXene-PC interfaces reshape the detectable plasma proteome and enable disease-associated molecular phenotyping, establishing a versatile nanointerface-driven framework for AD-related proteomic fingerprinting and molecular profiling. These results provide a foundation for future studies exploring MXene-based biointerfaces for plasma biomarker discovery and translational diagnostic applications. CI - (c) 2026 American Chemical Society. FAU - Velazquez, Samantha AU - Velazquez S AD - Department of Biology, University of Colorado Colorado Springs, Colorado Springs, CO80918, United States. FAU - Juber, Matthew C AU - Juber MC AD - Department of Medicine, University of Colorado School of Medicine, Aurora, CO80045, United States. FAU - Brindley, Dalton AU - Brindley D AD - Department of Physics and Energy Science, University of Colorado Colorado Springs, Colorado Springs, CO80918, United States. FAU - Thakur, Anupma AU - Thakur A AUID- ORCID: 0000-0001-7897-9229 AD - School of Materials Engineering, Purdue University, West Lafayette, IN47907, United States. FAU - Anasori, Babak AU - Anasori B AUID- ORCID: 0000-0002-1955-253X AD - School of Materials Engineering, Purdue University, West Lafayette, IN47907, United States. AD - School of Mechanical Engineering, Purdue University, West Lafayette, IN47907, United States. FAU - Lau, Edward AU - Lau E AUID- ORCID: 0000-0001-9083-5922 AD - Department of Medicine, University of Colorado School of Medicine, Aurora, CO80045, United States. FAU - Ashkarran, Ali Akbar AU - Ashkarran AA AUID- ORCID: 0000-0001-7883-6690 AD - Department of Physics and Energy Science, University of Colorado Colorado Springs, Colorado Springs, CO80918, United States. AD - BioFrontiers Center, University of Colorado Colorado Springs, Colorado Springs, CO80918, United States. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - ACS Appl Bio Mater JT - ACS applied bio materials JID - 101729147 SB - IM OTO - NOTNLM OT - Ti3C2Tx MXene OT - alzheimer disease OT - biomarker discovery OT - nanomaterials' protein corona OT - proteomics EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 01:03 PHST- 2026/06/14 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 01:03 [entrez] AID - 5460540 [pii] AID - 10.1021/acsabm.6c01228 [doi] PST - aheadofprint SO - ACS Appl Bio Mater. 2026 Oct 28. doi: 10.1021/acsabm.6c01228. PMID- 42952077 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2162-3279 (Electronic) VI - 16 IP - 11 DP - 2026 Nov TI - Sequential Associations of Sleep Architecture, Nocturnal Oxygenation, and Respiratory Disturbance With a Composite PSG-Derived Rhythm-Event Index. LID - e71882 [pii] LID - 10.1002/brb3.71882 [doi] AB - BACKGROUND: Sleep-disordered breathing (SDB), particularly obstructive sleep apnea (OSA), has been associated with cardiovascular complications including cardiac arrhythmias. However, the relative contributions of respiratory, oxygenation, and sleep architecture indices to composite PSG-derived rhythm-event index remain incompletely understood. OBJECTIVE: To investigate the sequential associations of sleep architecture, nocturnal oxygenation, and respiratory disturbance indices with the composite PSG-derived rhythm-event index in patients undergoing polysomnography (PSG). METHODS: Demographic, respiratory, oxygenation and sleep-architecture variables were extracted from PSG records. The primary outcome was a composite PSG-derived rhythm-event count, estimated as round(arrhythmia index x TST_hours) from the PSG-exported arrhythmia index, which was calculated during total sleep time only. Negative binomial regression used an offset of log(TST_hours). Because the outcome demonstrated overdispersion, negative binomial regression with an offset of log(TST hours) was used. Because the original 14-predictor model was over-parameterized for the sample size, a six-predictor model (age, sex, BMI, AHI, T90, and sleep efficiency) was chosen post hoc. This reduced model was not prespecified before the original analysis. Sleep efficiency was retained because it showed a strong signal in the exploratory 14-predictor model. The resulting p-values should therefore be interpreted as optimistic and exploratory. A sequential model-building approach (Model 1: age, sex, BMI, AHI; Model 2: plus T90; Model 3: plus sleep efficiency) was used to document the incremental contribution of each domain. A fully adjusted 14-predictor model was fitted only as a supplementary analysis. Wald 95% CIs and bootstrap percentile 95% CIs (2000 resamples) are reported. RESULTS: Forty-one participants were included (mean age 47.8 +/- 13.5 years; median BMI 29.4 kg/m(2)). Severe OSA (AHI >/= 30 events/hour) was present in 56.1%. Median arrhythmia index was 3.2 events/hour, with marked overdispersion. In the six-predictor model, only higher sleep efficiency was associated with lower arrhythmia rate (IRR = 0.946 per 1 percentage-point increase, 95% CI 0.921-0.972, p < 0.001; bootstrap 95% CI 0.888-0.984). T90 was associated in the Wald inference (IRR = 1.034, 95% CI 1.006-1.063, p = 0.016) but this association was not robust under bootstrap resampling (95% CI 0.979-1.067). AHI was not associated with arrhythmia rate (IRR = 1.000, 95% CI 0.987-1.014, p = 0.954). Age, sex and BMI were also not significant. A 14-predictor exploratory model had a marginally lower AIC (411.4) but a substantially higher BIC (438.8), consistent with overfitting. CONCLUSION: In this exploratory selected sleep-center cohort, AHI and other respiratory-event indices were not independently associated with the composite PSG-derived rhythm-event index. Only higher sleep efficiency showed a robust association with lower arrhythmia rate, but this finding is hypothesis-generating and may reflect unmeasured confounding. These results require confirmation in larger prospective studies and should not guide clinical risk stratification. CI - (c) 2026 The Author(s). Brain and Behavior published by Wiley Periodicals LLC. FAU - Nikoubakht, Nasim AU - Nikoubakht N AD - Department of Anesthesiology, School of Medicine, Hazrat-e Rasool General Hospital, Iran University of Medical Sciences, Tehran, Iran. FAU - Kashaninasab, Fatemeh AU - Kashaninasab F AD - Department of Psychiatry, Rasool Akram Medical Complex Clinical Research Development Center (RCRDC), Iran University of Medical Sciences, Tehran, Iran. FAU - Ghalehbandi, Mir Farhad AU - Ghalehbandi MF AD - Department of Psychiatry, School of Medicine, Mental Health Research Center, Psychosocial Health Research Institute, Iran University of Medical Sciences, Tehran, Iran. FAU - Khoozan, Mahboobeh AU - Khoozan M AD - Mental Health Research Center, Department of Psychiatry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. FAU - Nazari, Mohammad Ali AU - Nazari MA AD - School of Medicine, Iran University of Medical Sciences, Tehran, Iran. LA - eng PT - Journal Article PL - United States TA - Brain Behav JT - Brain and behavior JID - 101570837 RN - S88TT14065 (Oxygen) SB - IM MH - Humans MH - Polysomnography/methods MH - Female MH - Male MH - Middle Aged MH - *Sleep/physiology MH - *Sleep Apnea, Obstructive/physiopathology MH - *Sleep Apnea Syndromes/physiopathology MH - *Arrhythmias, Cardiac/physiopathology MH - *Oxygen/metabolism MH - Adult MH - Sleep Duration PMC - PMC13717065 COIS- The authors declare that they have no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/28 CRDT- 2026/10/29 01:03 PHST- 2026/10/13 00:00 [revised] PHST- 2026/05/31 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 01:03 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e71882 [pii] AID - BRB371882 [pii] AID - 10.1002/brb3.71882 [doi] PST - ppublish SO - Brain Behav. 2026 Nov;16(11):e71882. doi: 10.1002/brb3.71882. PMID- 42952065 OWN - NLM STAT- Publisher LR - 20261029 IS - 2234-8565 (Print) IS - 2234-8565 (Linking) DP - 2026 Oct 30 TI - Stent-assisted coiling versus simple coiling for ruptured intracranial aneurysms in the acute phase: A single-center retrospective cohort study. LID - 10.7461/jcen.2026.E2026.08.003 [doi] AB - OBJECTIVE: To compare intraprocedural complications, follow-up angiographic occlusion, and functional outcomes between stent-assisted coiling (SAC) and simple coiling for ruptured intracranial aneurysms treated during acute subarachnoid hemorrhage. METHODS: We retrospectively analyzed 217 patients treated from March 2018 to January 2026 (SAC, n=97; simple coiling, n=120). Follow-up angiographic analysis used the latest evaluable digital subtraction angiography (DSA) in survivors. Multivariable logistic regression evaluated intraprocedural complications, complete occlusion, and poor follow-up functional outcome; sensitivity analyses tested alternative covariate sets. RESULTS: Wide-neck morphology was more frequent in the SAC group (79.4% vs. 49.6%, p=5.71x10-6. Angiographically detected intraprocedural complications were more frequent after SAC (29.9% vs. 12.5%, p=0.002), and SAC remained associated with this endpoint in the primary model (adjusted odds ratio [aOR] 2.33, 95% confidence interval [CI] 1.07-5.08; p=0.033). The Raymond-Roy Occlusion Classification (RRC) distribution differed between groups (p=0.020), with complete occlusion in 43/54 (79.6%) versus 38/68 (55.9%). SAC was associated with complete occlusion after adjustment (aOR 2.59, 95% CI 1.06-6.32; p=0.037). Poor follow-up outcome did not differ (45.6% vs. 42.5%, p=0.672), and SAC was not independently associated with poor outcome. CONCLUSIONS: SAC was associated with a higher incidence of angiographically detected intraprocedural complications and more favorable follow-up angiographic occlusion in selected morphologically unfavorable ruptured aneurysms. A functional benefit was not demonstrated. FAU - Jung, Jiwon AU - Jung J AD - Department of Neurosurgery, Pusan National University Hospital, Busan, Korea. FAU - Ko, Jun Kyeung AU - Ko JK AD - Department of Neurosurgery, Pusan National University Hospital, Busan, Korea. redcheek09@naver.com. AD - Biomedical Research Institute, Pusan National University Hospital, Busan, Korea. redcheek09@naver.com. AD - School of Medicine, Pusan National University, Busan, Korea. redcheek09@naver.com. LA - eng PT - Journal Article DEP - 20261030 PL - Korea (South) TA - J Cerebrovasc Endovasc Neurosurg JT - Journal of cerebrovascular and endovascular neurosurgery JID - 101589154 OTO - NOTNLM OT - Embolization OT - Endovascular procedures OT - Intracranial aneurysm OT - Stents OT - Subarachnoid hemorrhage OT - Treatment outcome OT - therapeutic EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 01:00 PHST- 2026/08/02 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 01:00 [entrez] AID - jcen.2026.E2026.08.003 [pii] AID - 10.7461/jcen.2026.E2026.08.003 [doi] PST - aheadofprint SO - J Cerebrovasc Endovasc Neurosurg. 2026 Oct 30. doi: 10.7461/jcen.2026.E2026.08.003. PMID- 42952062 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1399-0012 (Electronic) IS - 0902-0063 (Linking) VI - 40 IP - 11 DP - 2026 Nov TI - Living Liver Donors With Obesity and Metabolic Risk: Review and Guidance on Donor Triage and Care. LID - e70681 [pii] LID - 10.1111/ctr.70681 [doi] AB - With the increasing prevalence of obesity in the United States, potential living liver donors (LLDs) are similarly impacted. Historically, LLDs with obesity are deemed too high risk for donation due to concern for hepatic steatosis (HS) or post-surgical complications. Evolving data show that obesity is not the absolute indicator of HS and metabolic risk. Instead, distribution of fat in the visceral area as well as body composition drive the risk for metabolic dysfunction associated steatotic liver disease (MASLD). Prior practice patterns of denial of obese LLDs with metabolic risk require a change in mindset from the liver transplant community with a thoughtful, individualized risk assessment of all LLDs. By losing weight utilizing various dietary and procedural strategies, these "gray zone" LLDs improve their metabolic risk profile and can move into a low risk "green zone", thus expanding the donor pool. Little data exist whether "gray zone" LLDs who succeed in weight loss have long-term consequences to their own health if they regain their weight or the clinical impact on their LDLT recipients. Ethical and financial questions remain as to the LDLT centers' direct responsibility to maintaining donor health. Future studies evaluating interventions and outcomes are needed. CI - (c) 2026 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. FAU - Han, Hyosun AU - Han H AD - Department of Medicine, USC Keck School of Medicine, Los Angeles, California, USA. FAU - Chacko, Kristina Rachel AU - Chacko KR AD - Department of Medicine, Albert Einstein College of Medicine, New York, New York, USA. FAU - Orandi, Babak AU - Orandi B AD - Department of Surgery, NYU Grossman School of Medicine, New York, New York, USA. FAU - von Ahrens, Dagny E AU - von Ahrens DE AD - Department of Surgery, Albert Einstein College of Medicine, New York, New York, USA. FAU - Shingina, Alexandra AU - Shingina A AD - Department of Medicine, University of Michigan School of Medicine, Ann Arbor, Michigan, USA. FAU - Kim, Susan C AU - Kim SC AD - Department of Surgery, Abdominal Transplantation, USC Keck School of Medicine, Los Angeles, California, USA. FAU - Watt, Kymberly D AU - Watt KD AD - Department of Medicine, Mayo Clinic, Rochester, Minnesota, USA. FAU - Liapakis, AnnMarie AU - Liapakis A AD - Department of Medicine, NYU Grossman School of Medicine, New York, New York, USA. CN - NALLDIG Consortium LA - eng PT - Journal Article PT - Review PL - Denmark TA - Clin Transplant JT - Clinical transplantation JID - 8710240 SB - IM MH - Humans MH - *Living Donors/supply & distribution MH - *Liver Transplantation MH - *Obesity/complications MH - Risk Factors MH - *Triage/standards MH - *Donor Selection/standards MH - *Practice Guidelines as Topic/standards MH - Prognosis MH - Fatty Liver OTO - NOTNLM OT - complication: medical/metabolic OT - donors and donation: living OT - liver transplantation: living donor OT - metabolic syndrome OT - obesity EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 CRDT- 2026/10/29 00:54 PHST- 2026/02/21 00:00 [revised] PHST- 2025/05/26 00:00 [received] PHST- 2026/08/27 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 00:54 [entrez] AID - e70681 [pii] AID - 10.1111/ctr.70681 [doi] PST - ppublish SO - Clin Transplant. 2026 Nov;40(11):e70681. doi: 10.1111/ctr.70681. PMID- 42952037 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1539-6304 (Electronic) IS - 1088-5412 (Linking) VI - 47 IP - 6 DP - 2026 Nov 1 TI - Adverse childhood experiences and allergic diseases: A scoping review. PG - 386-393 LID - 10.2500/aap.2026.47.260069 [doi] AB - Background: One in seven individuals experience at least four adverse childhood experiences (ACE). These experiences have been associated with poor health outcomes. Objectives: The objective was to map the association between ACEs and asthma, food allergy, allergic rhinitis, atopic dermatitis, urticaria, and mast cell activation syndrome. Methods: Original research that evaluated the association between ACEs and asthma, food allergy, allergic rhinitis, atopic dermatitis, urticaria, or mast cell activation syndrome was selected and abstracted. Review articles and non-peer-reviewed literature were excluded. Sources of evidence included several medical literature databases from inception to April 3, 2026. Each article was reviewed in duplicate at title and abstract, full text, and data extraction, with conflicts resolved by consensus or independent review. Quality assessment was conducted by using a modified assessment tool. Results: After duplicate removal (181 articles), 357 full-text articles were evaluated, with 267 studies excluded. The full text of 90 articles was assessed for eligibility, with 31 studies excluded (1 preprint, 11 wrong outcome, 1 wrong exposure, 17 wrong study design, 1 wrong population). The studies were subject to recall bias and limitations in terms of diagnostic accuracy of the outcome. Of 59 studies selected for inclusion in the review, 57 focused primarily on asthma/lung disease, 6 variably reported on poorly described allergy outcomes, and 2 reported on atopic dermatitis. No studies were identified that explored the association between ACEs and food allergy, urticaria, or mast cell activation syndrome. There is available evidence of an association of ACEs and asthma risk with a potential dose-response effect noted, indicating the possibility of a greater risk with greater ACE exposure. Conclusion: An association between ACEs and asthma is suggested. More research is needed to understand the impact of ACEs on other conditions evaluated by allergist/immunologists. FAU - Verdi, Marylee AU - Verdi M AD - From the Dartmouth College, Hanover, New Hampshire. FAU - White, Andrew A AU - White AA AD - Division of Allergy, Asthma, and Immunology, Scripps Clinic, San Diego, California. FAU - Sun, Michael AU - Sun M AD - Department of Psychological and Brain Sciences, Dartmouth College, Hanover, New Hampshire. FAU - Chapman, Natalie AU - Chapman N AD - Boston Children's Hospital, Boston, Massachusetts. FAU - Mahdavinia, Mahboobeh AU - Mahdavinia M AD - McGovern Medical School, University of Texas Health, Houston, Texas. FAU - Codispoti, Christopher D AU - Codispoti CD AD - McGovern Medical School, University of Texas Health, Houston, Texas. FAU - Shaker, Marcus S AU - Shaker MS AD - Section of Allergy and Immunology, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire. LA - eng PT - Journal Article PT - Scoping Review PL - United States TA - Allergy Asthma Proc JT - Allergy and asthma proceedings JID - 9603640 SB - IM MH - Humans MH - *Hypersensitivity/epidemiology/etiology MH - *Adverse Childhood Experiences/statistics & numerical data MH - Child MH - Asthma/epidemiology EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 CRDT- 2026/10/29 00:33 PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 00:33 [entrez] AID - 10.2500/aap.2026.47.260069 [doi] PST - ppublish SO - Allergy Asthma Proc. 2026 Nov 1;47(6):386-393. doi: 10.2500/aap.2026.47.260069. PMID- 42952025 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1442-2042 (Electronic) IS - 0919-8172 (Linking) VI - 33 IP - 11 DP - 2026 Nov TI - PSMA Theranostics for Prostate Cancer: Evidence, Pitfalls, and Implementation in Japan. LID - e70686 [pii] LID - 10.1111/iju.70686 [doi] AB - Prostate-specific membrane antigen (PSMA) is a Type II transmembrane glycoprotein markedly overexpressed on prostate cancer cells, enabling a single molecular target to support both positron emission tomography (PET) imaging and targeted radionuclide therapy. Over the past decade, PSMA-PET/CT has been incorporated into international guidelines for primary staging of high-risk prostate cancer and for biochemical recurrence, with the landmark proPSMA trial establishing its diagnostic superiority over conventional imaging; whether PSMA-PET-guided management improves long-term oncologic outcomes, however, remains an open question addressable only by prospective PSMA-specific trials. On the therapeutic side, (177)Lu-PSMA-617 has become a standard treatment option for metastatic castration-resistant prostate cancer (mCRPC) after androgen-receptor pathway inhibitor (ARPI) and taxane chemotherapy, and is moving into earlier lines of disease. Alpha-emitting and next-generation ligands, together with quantitative whole-body imaging and artificial intelligence, are redefining both patient selection and response assessment, although randomized evidence for many of these approaches remains limited. In Japan, the concurrent 2025 approval of (177)Lu-vipivotide tetraxetan and its companion PSMA diagnostic has initiated a national roll-out that will depend on a hub-and-spoke framework of regional PSMA-PET centers and therapy spokes, a prepared nuclear medicine workforce, and domestic registries to refine Japanese-specific evidence. This review summarizes the current clinical evidence for PSMA-PET/CT and (177)Lu-PSMA radioligand therapy, highlights interpretive and implementation pitfalls, and outlines practical priorities for the Japanese clinical and academic community. CI - (c) 2026 The Japanese Urological Association. FAU - Hirata, Kenji AU - Hirata K AUID- ORCID: 0000-0003-0036-8975 AD - Department of Diagnostic Imaging, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Hokkaido, Japan. AD - Department of Nuclear Medicine, Hokkaido University Hospital, Sapporo, Hokkaido, Japan. FAU - Watanabe, Shiro AU - Watanabe S AUID- ORCID: 0000-0002-7930-0975 AD - Department of Diagnostic Imaging, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Hokkaido, Japan. AD - Department of Nuclear Medicine, Hokkaido University Hospital, Sapporo, Hokkaido, Japan. FAU - Wakabayashi, Hiroshi AU - Wakabayashi H AUID- ORCID: 0000-0002-4942-6470 AD - Department of Nuclear Medicine, Kanazawa University Hospital, Kanazawa, Ishikawa, Japan. FAU - Kudo, Kohsuke AU - Kudo K AUID- ORCID: 0000-0001-5351-9242 AD - Department of Diagnostic Imaging, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Hokkaido, Japan. AD - Department of Diagnostic and Interventional Radiology, Hokkaido University Hospital, Sapporo, Hokkaido, Japan. FAU - Nishie, Akihiro AU - Nishie A AUID- ORCID: 0000-0001-7103-7757 AD - Department of Radiology, Graduate School of Medicine, University of the Ryukyus, Ginowan, Okinawa, Japan. LA - eng PT - Journal Article PT - Review PL - Australia TA - Int J Urol JT - International journal of urology : official journal of the Japanese Urological Association JID - 9440237 RN - 0 (Radiopharmaceuticals) RN - EC 3.4.17.21 (Glutamate Carboxypeptidase II) RN - 0 (Antigens, Surface) RN - EC 3.4.17.21 (FOLH1 protein, human) RN - 5H0DOZ21UJ (Lutetium) RN - 0 (Dipeptides) RN - 0 (Heterocyclic Compounds, 1-Ring) RN - 0 (PSMA-617) RN - EC 3.4.21.77 (Prostate-Specific Antigen) SB - IM MH - Humans MH - Male MH - Japan MH - *Radiopharmaceuticals/therapeutic use MH - *Glutamate Carboxypeptidase II/metabolism/analysis/antagonists & inhibitors MH - *Antigens, Surface/metabolism/analysis MH - Positron Emission Tomography Computed Tomography/methods MH - *Prostatic Neoplasms, Castration-Resistant/pathology/diagnostic imaging/therapy/drug therapy MH - Lutetium/therapeutic use MH - *Dipeptides/therapeutic use MH - Heterocyclic Compounds, 1-Ring/therapeutic use MH - *Prostatic Neoplasms MH - *Theranostic Nanomedicine/methods MH - Prostate/diagnostic imaging/pathology MH - Prostate-Specific Antigen OTO - NOTNLM OT - 177Lu-PSMA-617 OT - PET/CT OT - PSMA OT - prostate cancer OT - radioligand therapy OT - theranostics EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 CRDT- 2026/10/29 00:33 PHST- 2026/10/12 00:00 [revised] PHST- 2026/08/16 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 00:33 [entrez] AID - e70686 [pii] AID - 10.1111/iju.70686 [doi] PST - ppublish SO - Int J Urol. 2026 Nov;33(11):e70686. doi: 10.1111/iju.70686. PMID- 42952024 OWN - NLM STAT- Publisher LR - 20261029 IS - 1099-1573 (Electronic) IS - 0951-418X (Linking) DP - 2026 Oct 28 TI - Dihydroartemisinin Engages NQO1 and Promotes Ferroptosis-Related Oxidative Stress in Non-Small Cell Lung Cancer. LID - 10.1002/ptr.70497 [doi] AB - Dihydroartemisinin (DHA), a bioactive derivative of Artemisia annua L. (AAL), has shown antitumor activity in several malignancies, including non-small cell lung cancer (NSCLC). However, the molecular determinants linking DHA exposure to ferroptosis in NSCLC remain incompletely understood. This study aimed to identify functionally relevant targets of DHA-associated ferroptotic stress and to investigate the role of NAD(P)H quinone dehydrogenase 1 (NQO1) in this process. An integrated network pharmacology and transcriptomic approach was used to identify ferroptosis-related candidate targets of A. annua. Molecular docking and molecular dynamics simulations were used for target prioritization, followed by surface plasmon resonance (SPR) and cellular thermal shift assay (CETSA) to evaluate the interaction between DHA and NQO1. The effects of DHA on ferroptosis-related phenotypes and redox homeostasis were examined in A549 and PC9 cells. NQO1 overexpression, NQO1 knockdown, and GPX4 rescue experiments were performed to assess pathway involvement. Antitumor activity and NQO1-associated effects were further evaluated in mouse tumor models. Network pharmacology identified 15 active components of AAL and 61 NSCLC-associated therapeutic targets enriched in ROS-related and ferroptosis-associated pathways. Among 10 ferroptosis-related core targets, NQO1 was prioritized based on disease relevance, predicted DHA interaction, selective protein suppression, and complementary computational and experimental evidence. DHA was selected for mechanistic validation based on its pharmacological relevance and anti-NSCLC activity. SPR supported direct DHA-NQO1 binding (KD = 7.61 x 10(-6) M). Mechanistically, DHA suppressed NQO1 expression through transcriptional inhibition and enhanced protein degradation. DHA induced ferroptosis hallmarks, including ROS burst, LPO and Fe(2+) accumulation, GSH/GSSG depletion, MDA elevation, and GPX4 inactivation, in A549 and PC9 cells. NQO1 overexpression partially reversed these effects, whereas NQO1 knockdown phenocopied DHA-induced ferroptotic responses. GPX4 overexpression partially rescued the ferroptotic phenotype induced by NQO1 depletion, supporting a functional link between NQO1 and the GPX4 antioxidant axis. In vivo, DHA suppressed tumor growth, an effect partially reversed by NQO1 overexpression. DHA triggers ferroptosis in the NSCLC models examined, particularly in LUAD-derived cell models, in part through NQO1-mediated ROS burst and impairment of the GSH/GPX4 antioxidant defense. These findings identify NQO1 as a critical mediator of DHA-induced ferroptosis in these models and provide a mechanistic rationale for further investigation of DHA and related artemisinin derivatives in NSCLC. CI - (c) 2026 The Author(s). Phytotherapy Research published by John Wiley & Sons Ltd. FAU - Guo, Xiuli AU - Guo X AUID- ORCID: 0009-0001-7417-7461 AD - Department of Pulmonary Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Tao, Gan AU - Tao G AD - Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Zhang, Mini AU - Zhang M AD - Department of Pulmonary Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Han, Linzhi AU - Han L AUID- ORCID: 0009-0009-1443-767X AD - Department of Pulmonary Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Yan, Sisi AU - Yan S AD - Department of Pulmonary Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Wang, Chunsheng AU - Wang C AUID- ORCID: 0000-0003-4258-1790 AD - Department of Pulmonary Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Zhang, Jianguo AU - Zhang J AD - Department of Pulmonary Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Guo, Xiaowan AU - Guo X AD - Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Hu, Kehua AU - Hu K AD - Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Gong, Yan AU - Gong Y AUID- ORCID: 0000-0002-4805-0459 AD - Tumor Precision Diagnosis and Treatment Technology and Translational Medicine, Hubei Engineering Research Center, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - Hubei Key Laboratory of Tumor Biological Behavior, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Wu, Qiuji AU - Wu Q AUID- ORCID: 0000-0002-4655-3108 AD - Department of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - Hubei Key Laboratory of Tumor Biological Behavior, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Xie, Conghua AU - Xie C AUID- ORCID: 0000-0001-6623-9864 AD - Department of Pulmonary Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - Hubei Key Laboratory of Tumor Biological Behavior, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. LA - eng GR - 81803061/National Natural Science Foundation of China/ GR - 82473253/National Natural Science Foundation of China/ GR - WJ2023M068/Health Commission of Hubei Province Scientific Research Project/ GR - XKJS202005/Leading Discipline of Oncology Construction Project of Zhongnan Hospital of Wuhan University/ GR - ZNQNJC2022003/Youth Interdisciplinary Special Fund of Zhongnan Hospital of Wuhan University/ GR - 2023020201020510/Knowledge Innovation Program of Wuhan-Shuguang Project/ GR - ZNJC202322/Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University/ GR - ZNJC202312/Translational Medicine and Interdisciplinary Research Joint Fund of Zhongnan Hospital of Wuhan University/ GR - 2025LCYJZX-MS009/Clinical Research Special Fund of Zhongnan Hospital of Wuhan University/ GR - 2025LCYJZX-ZD005/Clinical Research Special Fund of Zhongnan Hospital of Wuhan University/ GR - 2042025YXB027/Fundamental Research Funds for the Central Universities ("Medicine + X")/ GR - Z-2017-24-2108/Immunoradiotherapy Research Fund of the Chinese Medical Association-Radiation Oncology Branch/ PT - Journal Article DEP - 20261028 PL - England TA - Phytother Res JT - Phytotherapy research : PTR JID - 8904486 SB - IM OTO - NOTNLM OT - GSH/GPX4 OT - NQO1 OT - NSCLC OT - dihydroartemisinin OT - ferroptosis EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 00:33 PHST- 2026/10/18 00:00 [revised] PHST- 2026/03/31 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 00:33 [entrez] AID - 10.1002/ptr.70497 [doi] PST - aheadofprint SO - Phytother Res. 2026 Oct 28. doi: 10.1002/ptr.70497. PMID- 42952013 OWN - NLM STAT- Publisher LR - 20261029 IS - 1097-0096 (Electronic) IS - 0091-2751 (Linking) DP - 2026 Oct 28 TI - Radiomic Signatures From Conventional MRI Predict Recovery in Obstetric Brachial Plexus Palsy: A Machine Learning Approach in Infants. LID - 10.1002/jcu.70453 [doi] AB - PURPOSE: Obstetric brachial plexus palsy (OBPP) causes variable neurological outcomes, but early prognostic biomarkers remain limited. This study evaluated whether radiomic features extracted from routine T2-STIR MRI can predict functional recovery in infants with OBPP, and whether combining these features with routinely available clinical predictors improves prognostic performance. METHODS: In this prospective single-center study, 39 infants with OBPP (mean age 12.5 +/- 5.7 weeks) underwent 3 T MRI during physiological sleep. Postganglionic lesions were semi-automatically segmented on coronal T2-STIR images, and 111 radiomic features were extracted per patient. After a minimum two-year clinical follow-up (mean 25.5 +/- 2.0 months; range 24-32 months), patients were classified as having complete (n = 17) or partial recovery (n = 22). Feature selection was performed using the Boruta algorithm. Five supervised machine learning models were trained and internally optimized on the training set (n = 28) using leave-one-out cross-validation (LOOCV), and their performance was subsequently evaluated on an independent holdout test set (n = 11). A clinical model (Narakas classification, birth weight, pseudomeningocele) and a combined clinical-radiomic model, summarized as a nomogram, were additionally developed. RESULTS: Eight radiomic features, including shape, first-order, and texture metrics, differentiated recovery groups. Among the radiomics-only models, SVM-RBF performed best (training AUC = 0.929, F1-score = 0.889; test AUC = 0.867, F1-score = 0.750), followed by random forest (training AUC = 0.952; test AUC = 0.783); test-set specificity was low for several classifiers. The combined clinical-radiomic model outperformed the radiomics-only model (training AUC = 1.000; test AUC = 0.933, accuracy = 0.818), and the bias-corrected calibration curve of the nomogram showed good agreement with observed outcomes (mean absolute error = 0.07). CONCLUSION: Radiomic analysis of routine T2-STIR MRI shows promise for predicting recovery in OBPP, and its combination with simple clinical predictors further improves discrimination. Given the small sample size and the performance drop between training and test sets, these findings should be regarded as preliminary and hypothesis-generating, and require validation in larger, multicenter cohorts before clinical use. CI - (c) 2026 Wiley Periodicals LLC. FAU - Cifci, Gokcen Coban AU - Cifci GC AUID- ORCID: 0000-0002-4010-2883 AD - Department of Radiology, Hacettepe University School of Medicine, Ankara, Turkiye. FAU - Avci, Hanife AU - Avci H AUID- ORCID: 0000-0002-1405-9754 AD - Department of Biostatistics, Hacettepe University School of Medicine, Ankara, Turkiye. FAU - Gumeler, Ekim AU - Gumeler E AUID- ORCID: 0000-0003-3783-2372 AD - Department of Radiology, Hacettepe University School of Medicine, Ankara, Turkiye. FAU - Karatas, Meltem AU - Karatas M AUID- ORCID: 0000-0003-2962-4573 AD - Bogazici University Institute of Biomedical Engineering, Istanbul, Turkiye. FAU - Uzumcugil, Akin AU - Uzumcugil A AD - Department of Orthopedics and Traumatology, Hacettepe University School of Medicine, Ankara, Turkiye. FAU - Firat, Tuzun AU - Firat T AUID- ORCID: 0000-0003-2200-7222 AD - Department of Physical Therapy and Rehabilitation, Hacettepe University, Ankara, Turkiye. FAU - Oguz, Kader Karli AU - Oguz KK AUID- ORCID: 0000-0002-3385-4665 AD - Department of Radiology, Division of Neuroradiology, UC Davis Medical Center, Sacramento, California, USA. FAU - Sengul, Gulgun AU - Sengul G AUID- ORCID: 0000-0002-5826-7379 AD - Department of Anatomy, Ege University, School of Medicine, Izmir, Turkiye. AD - Department of Neuroscience, Institute of Health Sciences, Ege University, Izmir, Turkiye. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Clin Ultrasound JT - Journal of clinical ultrasound : JCU JID - 0401663 SB - IM OTO - NOTNLM OT - MRI OT - machine learning OT - obstetric brachial plexus palsy OT - radiomics OT - recovery prediction EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 00:23 PHST- 2026/10/06 00:00 [revised] PHST- 2026/06/09 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 00:23 [entrez] AID - 10.1002/jcu.70453 [doi] PST - aheadofprint SO - J Clin Ultrasound. 2026 Oct 28. doi: 10.1002/jcu.70453. PMID- 42952008 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1550-6606 (Electronic) IS - 0022-1767 (Linking) VI - 215 IP - 10 DP - 2026 Oct 1 TI - Experimental high blood glucose metabolically rejuvenates CD8+ T cells for improved control of tumor growth through an IGF1-IGF1R axis. LID - vkag262 [pii] LID - 10.1093/jimmun/vkag262 [doi] AB - Epidemiologic studies suggest that patients with pre-existing type 1 diabetes have a decreased risk of developing melanoma, prostate cancer, and breast cancer, although the underlying mechanism remains unresolved. In translational model studies, we observed that a streptozotocin-induced high blood glucose model exhibited restricted melanoma and carcinoma (mammary, lung, and colon) growth in association with extended overall survival. Tumor-infiltrating CD8+ T cells were responsible for tumor growth restriction. Tumor-infiltrating CD8+ T cells but not tumor cells themselves exhibited higher glycolytic and cytotoxic activity in high-glucose hosts. Such improved antitumor T cell function was linked to upregulated expression of Igf1, Igf1R, and phospho-mTOR in CD8+ T cells in the tumor microenvironment. High blood glucose patient-derived peripheral CD8+ T cells displayed superior activation in vitro after allogenic tumor antigen stimulation versus nondiabetic CD8+ T cells. Activation of high-glucose-patient CD8+ T cells was sensitive to targeted antagonism of IGF1R and mTOR, supporting the operational involvement of the IGF1R-mTOR signaling axis. Our results suggest that selective activation of the intrinsic IGF1R-mTOR signaling axis in CD8+ T cells serves as a preferred endpoint to achieving more effective immunotherapy outcomes in cancer patients. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of The American Association of Immunologists. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Sarkar, Anirban AU - Sarkar A AD - Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata, India. FAU - Dhar, Sukanya AU - Dhar S AD - Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata, India. FAU - Bera, Saurav AU - Bera S AD - Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata, India. FAU - Chakravarti, Mohona AU - Chakravarti M AD - Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata, India. FAU - Verma, Ayushi AU - Verma A AD - Cancer Biology Division, CSIR-Central Drug Research Institute, Lucknow, India. FAU - Khan, Muqtada Ali AU - Khan MA AD - Cancer Biology Division, CSIR-Central Drug Research Institute, Lucknow, India. FAU - Prasad, Parash AU - Prasad P AD - Cell Biology and Physiology, CSIR-Indian Institute of Chemical Biology, Kolkata, India. FAU - Das, Anupam AU - Das A AD - Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Mohali, India. FAU - Choudhury, Pritha Roy AU - Choudhury PR AD - Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata, India. FAU - Sultana, Jasmine AU - Sultana J AD - Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata, India. FAU - Das, Juhina AU - Das J AD - Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata, India. FAU - Saha, Akata AU - Saha A AD - Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata, India. FAU - Bhuniya, Avishek AU - Bhuniya A AD - Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata, India. FAU - Guha, Ipsita AU - Guha I AD - Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata, India. FAU - Dasgupta, Shayani AU - Dasgupta S AD - Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata, India. FAU - Roy, Sib Sankar AU - Roy SS AD - Cell Biology and Physiology, CSIR-Indian Institute of Chemical Biology, Kolkata, India. FAU - Banerjee, Saptak AU - Banerjee S AD - Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata, India. FAU - Roy, Subir AU - Roy S AD - Department of Clinical Biochemistry, Doyen Diagnostics & Research Foundation, Kolkata, India. FAU - Bhar, Debarati AU - Bhar D AD - Department of Endocrinology, R. G. Kar Medical College and Hospital, Kolkata, India. FAU - Storkus, Walter J AU - Storkus WJ AD - Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, PA, United States. FAU - Baral, Rathindranath AU - Baral R AD - Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata, India. FAU - Datta, Dipak AU - Datta D AD - Cancer Biology Division, CSIR-Central Drug Research Institute, Lucknow, India. FAU - Bose, Anamika AU - Bose A AD - Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, Kolkata, India. AD - Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Mohali, India. LA - eng GR - University Grant Commission, New Delhi, Government of India/ GR - Indian Council of Medical Research, New Delhi, India/ GR - 45/04/2022-IMM/BMS/ PT - Journal Article PL - England TA - J Immunol JT - Journal of immunology (Baltimore, Md. : 1950) JID - 2985117R RN - 67763-96-6 (Insulin-Like Growth Factor I) RN - EC 2.7.10.1 (Receptor, IGF Type 1) RN - EC 2.7.11.1 (TOR Serine-Threonine Kinases) RN - 0 (Blood Glucose) RN - 0 (Igf1r protein, mouse) SB - IM MH - *CD8-Positive T-Lymphocytes/immunology/metabolism MH - Animals MH - *Insulin-Like Growth Factor I/metabolism MH - *Receptor, IGF Type 1/metabolism MH - Mice MH - Signal Transduction MH - Humans MH - TOR Serine-Threonine Kinases/metabolism MH - Tumor Microenvironment/immunology MH - Male MH - *Blood Glucose/metabolism MH - Lymphocytes, Tumor-Infiltrating/immunology/metabolism MH - Cell Line, Tumor MH - *Diabetes Mellitus, Experimental/immunology MH - Female MH - Mice, Inbred C57BL OTO - NOTNLM OT - CD8 T cells OT - high glucose model OT - insulin like growth factor 1 OT - insulin like growth factor 1 receptor OT - tumor microenvironment EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 CRDT- 2026/10/29 00:13 PHST- 2024/12/17 00:00 [received] PHST- 2026/08/09 00:00 [revised] PHST- 2026/08/28 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 00:13 [entrez] AID - 9003854 [pii] AID - 10.1093/jimmun/vkag262 [doi] PST - ppublish SO - J Immunol. 2026 Oct 1;215(10):vkag262. doi: 10.1093/jimmun/vkag262. PMID- 42952006 OWN - NLM STAT- Publisher LR - 20261029 IS - 1537-7385 (Electronic) IS - 0894-9115 (Linking) DP - 2026 Oct 29 TI - Effect of Incorporating Perceptual Learning Into Plantar Hardness Discrimination on the Risk of Falling and Muscle Torque in Patients With Hemiplegia: A Randomized Controlled Trial. LID - 10.1097/PHM.0000000000003194 [doi] AB - OBJECTIVES: This study aims to evaluate the impact of perceptual training involving plantar hardness discrimination on fall risk and muscle torque in individuals with hemiplegia. DESIGN: A randomized controlled trial was conducted in thirty-four stroke patients were randomly assigned to the study and control groups in equal numbers and assessed both before and after treatment. The outcome evaluations comprised fall risk assessment by the Biodex Balance System (BBS), the Isokinetic assessment of lower-limb muscle torque, and the Functional Independent Measures Scale (FIM). Each group was given a selected physical treatment program, while the study group also received perceptual learning with plantar hardness discrimination. RESULTS: Post-treatment comparison revealed that the study group exhibited a significantly lower risk of falling than the control group (P <0.001, effect size [ES]=1.48). Similarly, knee extensors torque increased significantly in the study group relative to the control group (P <0.001, ES=1.19). Additionally, the study group showed a significant rise in FIM scores compared with the control group (P <0.001, ES=1.56). CONCLUSIONS: For stroke patients, incorporating perceptual learning into plantar hardness discrimination in the rehabilitation program is regarded as beneficial physical therapy neurorehabilitation for enhancing risk of falling, lower limb muscle torque and function. CI - Copyright (c) 2026 Wolters Kluwer Health, LLC. All rights reserved. FAU - Mahmoud, Lama Saad El-Din AU - Mahmoud LSE AD - Assistant Professor, Department of Physical Therapy for Neurology and Neurosurgery, Faculty of Physical Therapy, October 6 University, Giza, Egypt. FAU - Mohamed, Hanaa S AU - Mohamed HS AD - Lecturer of physical therapy, Basic Science Department, Faculty of Physical Therapy, Modern University for Technology and Information, Cairo, Egypt. FAU - Shafeek, Marian M AU - Shafeek MM AD - Assistant professor of physical therapy for pediatrics and its surgery, Department of Physical Therapy for Pediatrics, Faculty of Physical Therapy, October University for Modern Sciences & Arts (MSA University), Giza, Egypt. FAU - Mahmud, Mohammed Saad El-Din AU - Mahmud MSE AD - Egyption Board of Neurology Higher Committee of Medical Specialies Egyption Fellowship Board, Cairo, Egypt. FAU - M Battesha, Hanan Hosny AU - M Battesha HH AUID- ORCID: 0000-0003-3242-4978 AD - Associate Professor, Physical Therapy Department, Faculty of Applied Medical Sciences, Al-Zaytoonah University of Jordan, Amman, Jordan. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Am J Phys Med Rehabil JT - American journal of physical medicine & rehabilitation JID - 8803677 SB - IM OTO - NOTNLM OT - Hemiplegia OT - Muscle Torque OT - Perception OT - Plantar Hardness Discrimination OT - Risk of Falling OT - Stroke COIS- Disclosures: The authors have no conflicts of interest or financial disclosures. Conflicts of interest: The authors do not have any conflict of interest to declare. Conflicts of interest: The authors have no relevant financial or non-financial interests to disclose. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 00:13 PHST- 2026/03/30 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 00:13 [entrez] AID - 00002060-990000000-01110 [pii] AID - 10.1097/PHM.0000000000003194 [doi] PST - aheadofprint SO - Am J Phys Med Rehabil. 2026 Oct 29. doi: 10.1097/PHM.0000000000003194. PMID- 42951966 OWN - NLM STAT- Publisher LR - 20261029 IS - 1476-4687 (Electronic) IS - 0028-0836 (Linking) DP - 2026 Oct 28 TI - Perinatal elimination of genetically aberrant human cortical neurons. LID - 10.1038/s41586-026-11190-3 [doi] AB - Human neurons are postmitotic and long-lived, requiring precise genomic regulation. Normal neuronal function is highly dosage-sensitive, with copy number variants (CNVs) and heterozygous point mutations associated with a host of neurodevelopmental and neuropsychiatric conditions(1-4). Here we investigated somatic CNVs arising in single neurons to understand the dynamic processes that shape the normal neuronal genetic landscape. Altogether, we sequenced 2,357 single neurons from human postmortem brain from fetal to postnatal life. Two single-cell whole-genome sequencing approaches-Tn5-transposase-based amplification (1,526 neurons, 16 individuals) and primary template amplification(5) (831 neurons, 12 individuals)-showed that an average of 25.9% [12.7-46%] of prenatal cortical neurons have highly aberrant genomes, that is, widespread CNVs involving multiple chromosomes. This proportion reduced by about 4.5-fold after birth across the two single-cell whole-genome sequencing approaches (Fisher meta-analysis P = 3.5 x 10(-)(6)). Neurons with widespread CNVs were mainly eliminated perinatally, timed roughly parallel to periods of programmed cell death(6), whereas neurons with smaller CNV burdens declined slowly in frequency with postnatal age. CNVs in surviving neurons were depleted for dosage-sensitive genes and genes involved in neurodevelopmental disorders (hypergeometric test P < 0.05), suggesting selective elimination of neurons with these CNVs. Analysis of 44,861 nuclei with single-nucleus assay for transposase-accessible chromatin (snATAC) and single-nucleus RNA sequencing (snRNA-seq) showed that neurons with high CNV burdens exhibited abnormal synaptic gene expression, suggesting that abnormal synaptic gene regulation contributes to neuronal elimination. Elimination of defective neuronal genomes might represent an important developmental genome quality-control process that may be vulnerable in neurodevelopmental disease, and suggests purifying selection during human neurodevelopment. CI - (c) 2026. The Author(s). FAU - Shao, Diane D AU - Shao DD AUID- ORCID: 0000-0003-2087-4082 AD - Department of Neurology, Boston Children's Hospital, Boston, MA, USA. AD - Department of Pediatrics, Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA, USA. FAU - Zhao, Yifan AU - Zhao Y AUID- ORCID: 0000-0003-4829-1428 AD - Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA. AD - Program in Health Sciences and Technology, Harvard Medical School and Massachusetts Institute of Technology, Boston, MA, USA. AD - Wellcome Sanger Institute, Hinxton, UK. AD - European Bioinformatics Institute, European Molecular Biology Laboratory, Hinxton, UK. FAU - Brew, Joseph AU - Brew J AUID- ORCID: 0009-0000-4752-0749 AD - Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA. FAU - Adla, Yahia AU - Adla Y AD - Department of Pediatrics, Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA, USA. AD - Howard Hughes Medical Institute, Chevy Chase, MD, USA. FAU - Ghosh, Urmi AU - Ghosh U AD - Department of Pediatrics, Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA, USA. AD - Howard Hughes Medical Institute, Chevy Chase, MD, USA. AD - Master of Public Health Program, School of Public Health, Brown University, Providence, RI, USA. FAU - Viswanadham, Vinayak V AU - Viswanadham VV AUID- ORCID: 0000-0002-0236-6490 AD - Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA. FAU - Luquette, Lovelace J AU - Luquette LJ AD - Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA. FAU - Zhao, Sijing AU - Zhao S AUID- ORCID: 0000-0003-1247-6834 AD - Department of Pediatrics, Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA, USA. AD - Biological and Biomedical Sciences Graduate Program, Harvard Medical School, Boston, MA, USA. FAU - Kriz, Andrea J AU - Kriz AJ AD - Department of Pediatrics, Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA, USA. FAU - Qian, Xuyu AU - Qian X AD - Department of Pediatrics, Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA, USA. AD - Howard Hughes Medical Institute, Chevy Chase, MD, USA. AD - Department of Pediatrics, Children's Hospital of Philadelphia, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. FAU - Taketomi, Takumi AU - Taketomi T AUID- ORCID: 0000-0003-1275-3563 AD - PhD Program in Human Biology, University of Tsukuba, Tsukuba, Japan. FAU - Coy, Shannon AU - Coy S AD - Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA. FAU - Santagata, Sandro AU - Santagata S AD - Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA. AD - Laboratory of Systems Pharmacology, Harvard Medical School, Boston, MA, USA. AD - Ludwig Center, Harvard University, Boston, MA, USA. FAU - Miller, Michael AU - Miller M AUID- ORCID: 0000-0002-6205-3314 AD - Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA. FAU - Tsuruta, Fuminori AU - Tsuruta F AUID- ORCID: 0000-0002-3471-7654 AD - PhD Program in Human Biology, University of Tsukuba, Tsukuba, Japan. AD - Institute of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan. FAU - Park, Peter J AU - Park PJ AUID- ORCID: 0000-0001-9378-960X AD - Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA. peter_park@hms.harvard.edu. FAU - Walsh, Christopher A AU - Walsh CA AUID- ORCID: 0000-0002-0156-2238 AD - Department of Neurology, Boston Children's Hospital, Boston, MA, USA. christopher.walsh@childrens.harvard.edu. AD - Department of Pediatrics, Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA, USA. christopher.walsh@childrens.harvard.edu. AD - Howard Hughes Medical Institute, Chevy Chase, MD, USA. christopher.walsh@childrens.harvard.edu. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Nature JT - Nature JID - 0410462 SB - IM COIS- Competing interests: P.J.P. is a member of the scientific advisory board (SAB) for Bioskryb Genomics. C.A.W. is a member of the SAB of Bioskryb Genomics (cash, equity), Mosaica Therapeutics (cash, equity), and an advisor to Maze Therapeutics (equity). EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 00:08 PHST- 2025/04/02 00:00 [received] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 00:08 [entrez] AID - 10.1038/s41586-026-11190-3 [pii] AID - 10.1038/s41586-026-11190-3 [doi] PST - aheadofprint SO - Nature. 2026 Oct 28. doi: 10.1038/s41586-026-11190-3. PMID- 42951963 OWN - NLM STAT- Publisher LR - 20261029 IS - 1476-4687 (Electronic) IS - 0028-0836 (Linking) DP - 2026 Oct 28 TI - Pharmacological inhibition of METTL1 as a therapy against solid and blood cancers. LID - 10.1038/s41586-026-11178-z [doi] AB - The N7-methylguanosine (m(7)G) modification is one of the most prevalent tRNA modifications, mainly catalysed by the METTL1-WDR4 protein complex(1-4). The m(7)G methyltransferase METTL1 has been linked to oncogenic transformation and the maintenance of several aggressive malignancies; however, the clinical implications of therapeutically targeting a tRNA-modifying enzyme have not been established. Here we present the development and characterization of STM9005, a highly selective and potent first-in-class tRNA methyltransferase inhibitor of METTL1. Treatment of numerous cancer cell models (including leukaemia, melanoma, pancreatic carcinoma, oesophageal cancer and small cell lung carcinoma) with STM9005 blocks cell growth, causes differentiation changes and dysregulates cell cycle progression. These cellular effects were accompanied by a selective decrease in m(7)G on a subset of tRNAs and a reduction in their expression levels, which impacted the translation of oncogenic mRNAs. We show that pharmacological inhibition of METTL1 in vivo impairs tumour growth and significantly prolongs survival in multiple mouse cancer models of different tissue origin. Collectively, our results provide a strong proof of concept that targeting of tRNA methylation is a promising avenue of anti-cancer therapy and illustrate that catalytic inhibition of METTL1 represents a therapeutic strategy against a number of aggressive malignancies. CI - (c) 2026. The Author(s). FAU - Yankova, Eliza AU - Yankova E AUID- ORCID: 0000-0003-2913-0012 AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. AD - Milner Therapeutics Institute, University of Cambridge, Cambridge, UK. FAU - Sapetschnig, Alexandra AU - Sapetschnig A AUID- ORCID: 0000-0001-8617-0235 AD - Storm Therapeutics, Cambridge, UK. FAU - Thomas, Beth AU - Thomas B AD - Storm Therapeutics, Cambridge, UK. FAU - Evans, Sian AU - Evans S AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. AD - Milner Therapeutics Institute, University of Cambridge, Cambridge, UK. FAU - Eleftheriou, Maria AU - Eleftheriou M AUID- ORCID: 0000-0002-1842-5838 AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. AD - Milner Therapeutics Institute, University of Cambridge, Cambridge, UK. FAU - Gozdecka, Malgorzata AU - Gozdecka M AUID- ORCID: 0000-0002-3134-1276 AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. FAU - Fischl, Harry AU - Fischl H AD - Storm Therapeutics, Cambridge, UK. FAU - Brevini, Teresa AU - Brevini T AUID- ORCID: 0000-0002-3581-5379 AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. FAU - Wilson, Rachael AU - Wilson R AUID- ORCID: 0000-0001-5129-7295 AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. FAU - Bucknell, Sarah J AU - Bucknell SJ AUID- ORCID: 0000-0002-6501-8899 AD - Storm Therapeutics, Cambridge, UK. FAU - Hutchin, Andrew AU - Hutchin A AUID- ORCID: 0000-0002-4961-6995 AD - Evotec, Abingdon, UK. FAU - Livi, Carmen M AU - Livi CM AD - Storm Therapeutics, Cambridge, UK. FAU - Giotopoulos, George AU - Giotopoulos G AUID- ORCID: 0000-0003-1390-6592 AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. FAU - Asby, Ryan AU - Asby R AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. FAU - Bond, Laura AU - Bond L AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. FAU - Azevedo, Aleksandra AU - Azevedo A AUID- ORCID: 0009-0007-2280-9483 AD - Storm Therapeutics, Cambridge, UK. FAU - Webster, Natalie AU - Webster N AD - Storm Therapeutics, Cambridge, UK. FAU - Rogan, Jack AU - Rogan J AD - Storm Therapeutics, Cambridge, UK. FAU - Russell, James AU - Russell J AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. FAU - Andrews, Byron AU - Andrews B AD - Storm Therapeutics, Cambridge, UK. FAU - Weisser, Hendrik AU - Weisser H AD - Storm Therapeutics, Cambridge, UK. FAU - Baxter, Joanna AU - Baxter J AD - Cambridge Blood and Stem Cell Biobank, Department of Haematology, University of Cambridge, Cambridge, UK. FAU - Lopez-Luis, Judith AU - Lopez-Luis J AUID- ORCID: 0000-0003-3202-9935 AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. AD - Milner Therapeutics Institute, University of Cambridge, Cambridge, UK. FAU - Mantica, Giovanna AU - Mantica G AUID- ORCID: 0000-0002-7429-7236 AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. FAU - Tobin, Jake AU - Tobin J AUID- ORCID: 0009-0001-2939-0896 AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. AD - Centre for Genomics Research, Discovery Sciences, BioPharmaceuticals R&D, AstraZeneca, Cambridge, UK. FAU - Lopez-Leon, Diego AU - Lopez-Leon D AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. FAU - Pask, Dean C AU - Pask DC AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. FAU - Tandon, Vidur AU - Tandon V AUID- ORCID: 0009-0001-4787-7825 AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. FAU - Fosbeary, Richard AU - Fosbeary R AD - Storm Therapeutics, Cambridge, UK. FAU - Sawyer, Sapphire AU - Sawyer S AUID- ORCID: 0009-0008-9281-1666 AD - Storm Therapeutics, Cambridge, UK. FAU - Usluer, Sunay AU - Usluer S AD - Wellcome Sanger Institute, Cambridge, UK. AD - Open Targets, Wellcome Genome Campus, Cambridge, UK. FAU - Joseph, Nithila AU - Joseph N AD - Wellcome Sanger Institute, Cambridge, UK. AD - Open Targets, Wellcome Genome Campus, Cambridge, UK. FAU - Stamou, Evangelia AU - Stamou E AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. AD - Milner Therapeutics Institute, University of Cambridge, Cambridge, UK. FAU - Bejar, Maria T AU - Bejar MT AUID- ORCID: 0000-0002-8850-0178 AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK. FAU - Malam, Yogeshkumar AU - Malam Y AUID- ORCID: 0000-0002-1192-7560 AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. FAU - Rad, Roland AU - Rad R AUID- ORCID: 0000-0002-6849-9659 AD - Institute of Molecular Oncology and Functional Genomics, School of Medicine, Technische Universitat Munchen, Munich, Germany. AD - Center for Translational Cancer Research (TranslaTUM), School of Medicine, Technische Universitat Munchen, Munich, Germany. AD - German Cancer Consortium (DKTK), Heidelberg, Germany. AD - Department of Medicine II, Klinikum rechts der Isar, School of Medicine, Technische Universitat Munchen, Munich, Germany. FAU - Laurenti, Elisa AU - Laurenti E AUID- ORCID: 0000-0002-9917-9092 AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. FAU - Li, Juan AU - Li J AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. FAU - Alcolea, Maria P AU - Alcolea MP AUID- ORCID: 0000-0002-8396-6454 AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK. FAU - Adams, David J AU - Adams DJ AUID- ORCID: 0000-0001-9490-0306 AD - Wellcome Sanger Institute, Cambridge, UK. FAU - Khaled, Walid T AU - Khaled WT AUID- ORCID: 0000-0001-9068-5776 AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Pharmacology, University of Cambridge, Cambridge, UK. FAU - Sampaziotis, Fotios AU - Sampaziotis F AUID- ORCID: 0000-0003-0812-7586 AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Cambridge Liver Unit, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK. AD - National Institute of Health Research (NIHR) Blood and Transplant Research Unit (BTRU) at the University of Cambridge in collaboration with Newcastle University and in partnership with NHS Blood and Transplant (NHSBT), Cambridge, UK. AD - Department of Medicine, University of Cambridge, Cambridge, UK. FAU - Vassiliou, George S AU - Vassiliou GS AUID- ORCID: 0000-0003-4337-8022 AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. FAU - Huntly, Brian J P AU - Huntly BJP AUID- ORCID: 0000-0003-0312-161X AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. AD - Department of Haematology, University of Cambridge, Cambridge, UK. FAU - Rausch, Oliver AU - Rausch O AUID- ORCID: 0000-0003-4074-3848 AD - Storm Therapeutics, Cambridge, UK. oliver.rausch@catalyst-bio.co.uk. FAU - Tzelepis, Konstantinos AU - Tzelepis K AUID- ORCID: 0000-0002-4865-7648 AD - Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK. kt404@cam.ac.uk. AD - Department of Haematology, University of Cambridge, Cambridge, UK. kt404@cam.ac.uk. AD - Milner Therapeutics Institute, University of Cambridge, Cambridge, UK. kt404@cam.ac.uk. AD - Wellcome Sanger Institute, Cambridge, UK. kt404@cam.ac.uk. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Nature JT - Nature JID - 0410462 SB - IM COIS- Competing interests: K.T. has received stock options, consultancy fees and research funding from Storm Therapeutics, and is a stockholder of TEP Therapeutics. A.S., B.T., H.F., S.J.B., H.W., C.M.L., A.A., N.W., J. Rogan, B.A., R.F., S.S. and O.R. were employees of Storm Therapeutics at the time the experiments were performed. G.S.V. is a consultant to STRM.BIO and holds a research grant from AstraZeneca for research unrelated to that presented here. J.T. receives research funding from AstraZeneca. E.L. holds a research grant from CSL Behring for research unrelated to that presented here. The other authors declare no competing interests. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 00:08 PHST- 2025/07/23 00:00 [received] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 00:08 [entrez] AID - 10.1038/s41586-026-11178-z [pii] AID - 10.1038/s41586-026-11178-z [doi] PST - aheadofprint SO - Nature. 2026 Oct 28. doi: 10.1038/s41586-026-11178-z. PMID- 42951951 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1476-4687 (Electronic) IS - 0028-0836 (Print) IS - 0028-0836 (Linking) VI - 658 IP - 8138 DP - 2026 Oct TI - A single-molecule spin-photon interface. PG - 1153-1159 LID - 10.1038/s41586-026-11147-6 [doi] AB - Optical interfaces that connect long-lived spin qubits to photons are a central requirement for quantum networking and distributed quantum information processing. At present, solid-state atomic defects are leading candidates because of their inherent spin and optical coherence(1,2). Building on these advancements, synthetically tailored molecular systems represent a fundamental change in the field, using precise atomic control and consistent bottom-up assembly(3,4). However, a molecular platform that concurrently satisfies the demands of a robust spin-photon interface-namely, the combination of bright fluorescence, high spectral stability and long ground-state spin lifetimes-has yet to be realized at the single-molecule level. Here we show that a triplet ground-state carbene molecule, embedded within a structurally matched host crystal, functions as a robust spin-photon interface with single-molecule addressability. The system exhibits narrow ZPLs, spectral stability over more than an hour, spin-selective optical transitions and single-molecule optically detected magnetic resonance. Coherent control yields millisecond-scale dynamical-decoupling coherence and tens-of-milliseconds spin relaxation at a temperature of 4.5 K. These results establish molecular qubits as a viable platform for single-emitter quantum optics(5,6) while preserving the advantages of bottom-up chemical design(7) and processable materials(8-10). CI - (c) 2026. The Author(s). FAU - Roggors, Simon AU - Roggors S AD - NVision Quantum Technologies, Ulm, Germany. AD - Institute for Quantum Optics (IQO), Ulm University, Ulm, Germany. FAU - Unden, Thomas AU - Unden T AD - NVision Quantum Technologies, Ulm, Germany. FAU - Aubele, Anna AU - Aubele A AD - NVision Quantum Technologies, Ulm, Germany. FAU - Mentzel, Paul AU - Mentzel P AD - NVision Quantum Technologies, Ulm, Germany. FAU - Bayer, Gregor AU - Bayer G AD - NVision Quantum Technologies, Ulm, Germany. FAU - Salhov, Alon AU - Salhov A AUID- ORCID: 0000-0001-9111-2400 AD - NVision Quantum Technologies, Ulm, Germany. AD - Racah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem, Israel. FAU - Scharpf, Jochen AU - Scharpf J AD - NVision Quantum Technologies, Ulm, Germany. FAU - Plenio, Martin B AU - Plenio MB AUID- ORCID: 0000-0003-4238-8843 AD - Institute of Theoretical Physics, Ulm University, Ulm, Germany. AD - Center for Integrated Quantum Science and Technology (IQST), Ulm University, Ulm, Germany. FAU - Retzker, Alex AU - Retzker A AUID- ORCID: 0000-0002-7291-7120 AD - Racah Institute of Physics, The Hebrew University of Jerusalem, Jerusalem, Israel. FAU - Jelezko, Fedor AU - Jelezko F AD - Institute for Quantum Optics (IQO), Ulm University, Ulm, Germany. AD - Center for Integrated Quantum Science and Technology (IQST), Ulm University, Ulm, Germany. FAU - Eichhorn, Tim R AU - Eichhorn TR AD - NVision Quantum Technologies, Ulm, Germany. FAU - Schaub, Tobias A AU - Schaub TA AD - NVision Quantum Technologies, Ulm, Germany. AD - Institute for Quantum Optics (IQO), Ulm University, Ulm, Germany. FAU - Pfender, Matthias AU - Pfender M AUID- ORCID: 0000-0003-3934-8306 AD - NVision Quantum Technologies, Ulm, Germany. matthias@nvision-quantum.com. FAU - Neumann, Philipp AU - Neumann P AD - NVision Quantum Technologies, Ulm, Germany. FAU - Schwartz, Ilai AU - Schwartz I AD - NVision Quantum Technologies, Ulm, Germany. ilai@nvision-quantum.com. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Nature JT - Nature JID - 0410462 RN - OP0UW79H66 (Methane) RN - 2465-56-7 (carbene) SB - IM MH - *Photons MH - *Quantum Theory MH - *Methane/analogs & derivatives/chemistry MH - Temperature MH - *Quantum Mechanics PMC - PMC13716814 COIS- Competing interests: The authors declare the following competing financial interests: S.R., T.U., A.A., P.M., G.B., A.S., J.S., T.R.E., T.A.S., M.P., P.N. and I.S. are employees of NVision Quantum Technologies (NVision). M.B.P., A.R., F.J. and I.S. are shareholders of NVision. NVision is commercializing products in the field of quantum information technology. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/28 CRDT- 2026/10/29 00:08 PHST- 2026/04/08 00:00 [received] PHST- 2026/09/10 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 00:08 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 10.1038/s41586-026-11147-6 [pii] AID - 11147 [pii] AID - 10.1038/s41586-026-11147-6 [doi] PST - ppublish SO - Nature. 2026 Oct;658(8138):1153-1159. doi: 10.1038/s41586-026-11147-6. Epub 2026 Oct 28. PMID- 42951946 OWN - NLM STAT- Publisher LR - 20261029 IS - 1538-4683 (Electronic) IS - 1061-5377 (Linking) DP - 2026 Oct 29 TI - Artificial Intelligence Applied to Echocardiography for Detection of Heart Failure with Preserved Ejection Fraction and Elevated Filling Pressures: A Diagnostic Test Accuracy Meta-Analysis. LID - 10.1097/CRD.0000000000001523 [doi] AB - Heart failure with preserved ejection fraction (HFpEF) accounts for nearly half of heart failure cases and remains difficult to diagnose non-invasively. Artificial intelligence (AI) applied to echocardiography may improve detection, but its aggregate diagnostic accuracy has not been quantified. We performed a diagnostic test accuracy meta-analysis of AI-enabled echocardiography for identifying HFpEF, elevated left ventricular filling pressure, or diastolic dysfunction. MEDLINE/PubMed, Embase, and CENTRAL were searched from inception to June 2026. Studies reporting the accuracy of AI models applied to echocardiography against invasive, guideline-adjudicated, or ASE/EACVI reference standards were included. Sensitivity and specificity were pooled using a bivariate random-effects (Reitsma) model, with a summary receiver operating characteristic (ROC) curve, diagnostic odds ratio, and likelihood ratios. Risk of bias was assessed with QUADAS-2. Eight studies (2770 participants; 1317 [47.5%] with the target condition) were included. Pooled sensitivity was 84.0% (95% CI: 73.0-91.0%) and specificity 83.0% (95% CI: 73.0-89.0%), with an area under the summary ROC curve of 0.875 (95% CI: 0.791-0.930). The diagnostic odds ratio was 24.73 (95% CI: 18.46-33.12); positive and negative likelihood ratios were 4.84 and 0.20. Heterogeneity was high. Performance did not differ by AI architecture but varied significantly by reference standard. No small-study effects were detected. AI-enabled echocardiography shows good pooled accuracy for detecting HFpEF and elevated filling pressures, supporting its diagnostic potential. High heterogeneity and reference-standard dependence warrant larger, prospectively validated studies before routine clinical adoption. CI - Copyright (c) 2026 Wolters Kluwer Health, LLC. All rights reserved. FAU - A Al-Harbi, Faisal AU - A Al-Harbi F AD - From the Department of Medicine, College of Medicine, Qassim University, Buraidah 51452, Saudi Arabia. FAU - Alawaji, Hatim Hussain AU - Alawaji HH AD - From the Department of Medicine, College of Medicine, Qassim University, Buraidah 51452, Saudi Arabia. FAU - Alhabdan, Hamad Suliman AU - Alhabdan HS AD - From the Department of Medicine, College of Medicine, Qassim University, Buraidah 51452, Saudi Arabia. FAU - Alsultan, Norah Sulaiman AU - Alsultan NS AD - From the Department of Medicine, College of Medicine, Qassim University, Buraidah 51452, Saudi Arabia. FAU - Alrebdi, Dana Abdullah AU - Alrebdi DA AD - From the Department of Medicine, College of Medicine, Qassim University, Buraidah 51452, Saudi Arabia. FAU - AlMutairi, Osamah Hamdi AU - AlMutairi OH AD - From the Department of Medicine, College of Medicine, Qassim University, Buraidah 51452, Saudi Arabia. FAU - Alharbi, Faisal Mohammed AU - Alharbi FM AD - From the Department of Medicine, College of Medicine, Qassim University, Buraidah 51452, Saudi Arabia. FAU - Alosail, Abdulmajeed Faleh AU - Alosail AF AD - Department of Internal Medicine, College of Medicine, King Faisal University, Al-Ahsa, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Cardiol Rev JT - Cardiology in review JID - 9304686 SB - IM OTO - NOTNLM OT - artificial intelligence OT - echocardiography OT - heart failure with preserved ejection fraction COIS- Disclosure: The authors declare no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 00:07 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 00:07 [entrez] AID - 00045415-990000000-01062 [pii] AID - 10.1097/CRD.0000000000001523 [doi] PST - aheadofprint SO - Cardiol Rev. 2026 Oct 29. doi: 10.1097/CRD.0000000000001523. PMID- 42951939 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1742-481X (Electronic) IS - 1742-4801 (Print) IS - 1742-4801 (Linking) VI - 23 IP - 11 DP - 2026 Nov TI - Combined Use of Adhesive Mesh Closure and Closed-Incision Negative Pressure Therapy for High-Risk Surgical Wounds: A Proof-Of-Concept Study. LID - e71089 [pii] LID - 10.1111/iwj.71089 [doi] AB - Surgical wound complications remain a major challenge, particularly in high-risk patients. Closed-incision negative pressure wound therapy (ciNPWT) and adhesive mesh closure systems have shown benefits independently, but their combined use has not been previously evaluated. This single-centre prospective proof-of-concept study included consecutive patients at extremely high risk of wound complications undergoing complex surgical procedures. All patients were treated with adhesive mesh closure (Dermabond Prineo) and ciNPWT (PICO) using a standardized protocol. Outcomes were compared with a historical cohort treated with conventional skin closure. Primary outcomes were wound healing and surgical site complications; secondary outcomes included persistent drainage, delayed healing, readmissions and duration of therapy. Seventeen surgical procedures (16 patients) were included in the prospective cohort and 39 in the historical cohort. The combined strategy achieved consistent wound healing without device-related or allergic complications. Compared with controls, no significant difference was observed for the composite outcome of surgical site complications (17.6% vs. 23.1%), although lower rates were observed for persistent drainage (0 vs. 23.1%, p = 0.045), delayed healing (5.9% vs. 20.5%) and readmissions (0 vs. 20.5%); only persistent drainage reached statistical significance. ciNPWT duration was shorter (12.3 vs. 17.3 days; p = 0.006). This combined approach appears feasible and safe in a highly selected cohort of high-risk patients, with preliminary findings suggesting potential reductions in wound-related complications. CI - (c) 2026 The Author(s). International Wound Journal published by Medicalhelplines.com Inc and John Wiley & Sons Ltd. FAU - Sanz-Ruiz, Pablo AU - Sanz-Ruiz P AUID- ORCID: 0000-0002-7588-1880 AD - Department of Orthopaedic Surgery and Traumatology, General University Hospital Gregorio Maranon, Madrid, Spain. AD - Faculty of Medicine, Complutense University of Madrid, Madrid, Spain. AD - Instituto de Investigacion Sanitaria Gregorio Maranon (IiSGM), Madrid, Spain. FAU - Marquez-Gomez, Miguel AU - Marquez-Gomez M AUID- ORCID: 0000-0001-8876-6868 AD - Department of Orthopaedic Surgery and Traumatology, General University Hospital Gregorio Maranon, Madrid, Spain. AD - Instituto de Investigacion Sanitaria Gregorio Maranon (IiSGM), Madrid, Spain. FAU - Peinado, Lourdes Prats AU - Peinado LP AUID- ORCID: 0000-0003-4109-3373 AD - Department of Orthopaedic Surgery and Traumatology, General University Hospital Gregorio Maranon, Madrid, Spain. FAU - Ayuso, Alejandro Barrios AU - Ayuso AB AUID- ORCID: 0009-0004-9310-3291 AD - Department of Orthopaedic Surgery and Traumatology, General University Hospital Gregorio Maranon, Madrid, Spain. FAU - Bueno, Juan Dimas AU - Bueno JD AUID- ORCID: 0009-0001-8981-7943 AD - Department of Orthopaedic Surgery and Traumatology, General University Hospital Gregorio Maranon, Madrid, Spain. FAU - Vaquero, Javier AU - Vaquero J AD - Department of Orthopaedic Surgery and Traumatology, General University Hospital Gregorio Maranon, Madrid, Spain. AD - Faculty of Medicine, Complutense University of Madrid, Madrid, Spain. AD - Instituto de Investigacion Sanitaria Gregorio Maranon (IiSGM), Madrid, Spain. LA - eng PT - Journal Article PL - England TA - Int Wound J JT - International wound journal JID - 101230907 SB - IM MH - Humans MH - *Negative-Pressure Wound Therapy/methods MH - Female MH - *Wound Healing/physiology MH - Prospective Studies MH - Male MH - Aged MH - Middle Aged MH - *Surgical Mesh MH - *Surgical Wound/therapy MH - *Surgical Wound Infection/prevention & control/therapy MH - Proof of Concept Study MH - Aged, 80 and over MH - Adult MH - Treatment Outcome MH - *Wound Closure Techniques PMC - PMC13716930 OTO - NOTNLM OT - bandages OT - negative-pressure wound therapy OT - surgical wound dehiscence OT - surgical wound infection OT - wound healing COIS- The authors declare no conflicts of interest with respect to the research, authorship or publication of this article. The devices used (Dermabond Prineo, Ethicon Inc. and PICO, Smith & Nephew) were procured through standard institutional channels. The manufacturers had no role in study design, data collection, analysis, interpretation or manuscript preparation. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/28 CRDT- 2026/10/29 00:07 PHST- 2026/09/12 00:00 [revised] PHST- 2026/04/06 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 00:07 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e71089 [pii] AID - IWJ71089 [pii] AID - 10.1111/iwj.71089 [doi] PST - ppublish SO - Int Wound J. 2026 Nov;23(11):e71089. doi: 10.1111/iwj.71089. PMID- 42951925 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2471-254X (Electronic) IS - 2471-254X (Linking) VI - 10 IP - 11 DP - 2026 Nov 1 TI - Clinical, safety, and patient-reported outcomes associated with second-line and later therapies in primary biliary cholangitis: A systematic review. LID - 10.1097/HC9.0000000000001048 [doi] LID - e01048 AB - BACKGROUND: Primary biliary cholangitis (PBC) is a rare, chronic, cholestatic disease that can result in cirrhosis, liver failure, and death. While biochemical markers such as alkaline phosphatase (ALP) and total bilirubin (TB) measure disease activity and progression, definitions of biochemical response vary. This systematic literature review (SLR) identified clinical studies for investigational and approved treatments in patients with PBC with inadequate response or intolerance to ursodeoxycholic acid requiring second-line (2L) or later (2L+) therapies. METHODS: Electronic databases were searched on August 7, 2025 (MEDLINE; Embase) or September 30, 2025 (Cochrane Database of Systematic Reviews; CENTRAL), and conference proceedings (2021-2025), bibliographies, and ClinicalTrials.gov were searched on August 15, 2025, following a pre-specified protocol (PROSPERO ID: CRD42023382262). Interventional and observational studies in the 2L/2L+ setting were prioritized for inclusion, and randomized controlled trials for extraction. RESULTS: A total of 149 publications on 44 unique studies were included. Phase III trials evaluated elafibranor, seladelpar, budenoside, bezafibrate, and obeticholic acid. Various composite biochemical response endpoints were reported across 34% of studies, most frequently the POISE criteria (18%). Biochemical markers were assessed frequently (ALP: 89%; TB: 71%); however, upper limits of normal and response thresholds varied substantially. Response rates in placebo-controlled trials favored active treatment, although placebo responses were highly variable. Non-invasive fibrosis measures were reported in 16% of studies and showed small short-term changes. Patient-reported outcomes assessed pruritus (52%) and fatigue (32%) using a variety of scales. CONCLUSION: The evidence base for 2L/2L+ PBC therapies is extensive but heterogeneous in study designs, patient populations, and treatment assessment scales and outcome definitions, limiting cross-trial comparisons. This SLR provides a robust foundation for contextualizing study outcomes within the broader landscape and supporting clinical decision-making. CI - Copyright (c) 2026 The Author(s). Published by Wolters Kluwer Health, LLC. on behalf of the American Association for the Study of Liver Diseases. FAU - Carbone, Marco AU - Carbone M AD - Division of Gastroenterology and Center for Autoimmune Liver Diseases, Department of Medicine and Surgery, University of Milan-Bicocca, Milan, Italy. FAU - Jones, David AU - Jones D AD - Institute of Cellular Medicine and NIHR Newcastle Biomedical Research Centre, Newcastle University, Newcastle Upon Tyne, UK. FAU - Solanke, Oyinkan AU - Solanke O AD - Ipsen, London, UK. FAU - Brandt, Rikke AU - Brandt R AD - Ipsen, London, UK. FAU - Krause, Taryn AU - Krause T AD - Ipsen, London, UK. FAU - Asquith, Darren AU - Asquith D AD - Ipsen, London, UK. FAU - Pashley, Alex AU - Pashley A AD - Costello Medical, Cambridge, UK. FAU - Shaw, Sonia AU - Shaw S AD - Costello Medical, Cambridge, UK. FAU - Worthington, Emma AU - Worthington E AD - Costello Medical, Cambridge, UK. FAU - Schattenberg, Jorn M AU - Schattenberg JM AD - Department of Internal Medicine II, Saarland University Medical Center, Homburg, Germany. AD - Pharma Science Hub Saarbrucken, Germany. LA - eng PT - Journal Article PT - Systematic Review DEP - 20261014 PL - United States TA - Hepatol Commun JT - Hepatology communications JID - 101695860 RN - 724L30Y2QR (Ursodeoxycholic Acid) RN - 0 (Cholagogues and Choleretics) RN - 0GEI24LG0J (Chenodeoxycholic Acid) RN - 0462Z4S4OZ (obeticholic acid) RN - Y9449Q51XH (Bezafibrate) SB - IM MH - Humans MH - *Liver Cirrhosis, Biliary/drug therapy MH - *Patient Reported Outcome Measures MH - Ursodeoxycholic Acid/therapeutic use MH - *Cholagogues and Choleretics/therapeutic use MH - Treatment Outcome MH - Chenodeoxycholic Acid/analogs & derivatives/therapeutic use MH - Bezafibrate/therapeutic use PMC - PMC13668302 OTO - NOTNLM OT - biliary cholangitis OT - biological markers OT - clinical decision-making OT - fibrosis OT - pruritus OT - research design OT - second-line therapy COIS- Marco Carbone: served as advisor for Ipsen, Advanz, AlphaSigma, Amgen, Intercept, Cymabay, Gilead, GlaxoSmithKline, Mayoly, Echosens, Falk, Mirum, Kowa, and Zydus; received support for attending meetings and/or travel from Advanz, CymaBay, and Ipsen. David Jones: received consulting fees from Intercept, Ipsen, and Umecrine; received payment or honoraria from GlaxoSmithKline and Ipsen; received support for attending meetings and/or travel from Advanz; participated on a Data Safety Monitoring Board or Advisory Board for CymaBay and Ipsen. Oyinkan Solanke, Rikke Brandt, and Taryn Krause: employees of Ipsen. Darren Asquith: employee and shareholder of Ipsen. Alex Pashley, Sonia Shaw, and Emma Worthington: employees of Costello Medical. Jorn M. Schattenberg: received consultant honoraria from Akero, Alexion, Altimmune, Astra Zeneca, 89Bio, Boehringer Ingelheim, Gilead Sciences, GSK, Ipsen, Inventiva Pharma, Madrigal Pharmaceuticals, Merck Kriya Therapeutics, Echosens, Eli Lilly, E-Therapeutics, Merck, MSD Sharp & Dohme GmbH, Novo Nordisk, Roche, and Vantage Biosciences Ltd; received speaker honoraria from AbbVie, Boehringer Ingelheim, Gilead Sciences, Ipsen, Lilly, Madrigal Pharmaceuticals, MSD, Novo Nordisk, and Opella; received travel support from Boehringer Ingelheim, Novo Nordisk, and Ipsen; stockholder options: Hepta Bio and HeapCure Pharma. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/14 CRDT- 2026/10/29 00:07 PHST- 2026/01/22 00:00 [received] PHST- 2026/07/17 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 00:07 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 02009842-202611010-00001 [pii] AID - HEP4-26-0126 [pii] AID - 10.1097/HC9.0000000000001048 [doi] PST - epublish SO - Hepatol Commun. 2026 Oct 14;10(11):e01048. doi: 10.1097/HC9.0000000000001048. eCollection 2026 Nov 1. PMID- 42951905 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2471-254X (Electronic) IS - 2471-254X (Linking) VI - 10 IP - 11 DP - 2026 Nov 1 TI - Machine learning models of noninvasive tests to predict metabolic dysfunction-associated steatohepatitis and fibrosis stage. LID - 10.1097/HC9.0000000000001064 [doi] LID - e01064 AB - BACKGROUND: Fibrosis stage in metabolic dysfunction-associated steatohepatitis (MASH) is currently diagnosed by liver biopsy in clinical trials. Using noninvasive tests, no consensus method exists for staging fibrosis or diagnosing MASH. We aimed to develop machine learning (ML) methods to predict fibrosis stage and MASH with F2/F3 fibrosis using noninvasive biomarkers. METHODS: Data from 1970 biopsy-confirmed patients screened for phase 3 resmetirom trials (MAESTRO-NASH, MAESTRO-NAFLD-1) were retrospectively analyzed. Outcomes were fibrosis stage and MASH with F2/F3 fibrosis [NAFLD Activity Score (NAS) >/=4 with moderate to advanced noncirrhotic fibrosis], modeled from 37 clinical, laboratory, and imaging predictors. Random forest models were developed, including a full model using all 37 predictors and "Lean" models using subsets of predictors routinely available in clinical practice. These models were evaluated by 4-fold cross-validation using AUC. RESULTS: For three-class fibrosis staging (F0/F1 vs. F2/F3 vs. F4), one-versus-rest cross-validation mean AUCs were 0.76, 0.75, and 0.94 for the full model and 0.83, 0.78, and 0.89 for the best-performing Lean model. Liver stiffness measures (LSM) and MASH composite algorithms, such as the Fibrosis-4 Index (FIB-4) and FibroScan-AST, were among the most important predictors. For MASH with F2/F3 fibrosis, the full and best-performing Lean models achieved AUCs of 0.75 and 0.79, respectively. Parsimonious top-k models retaining LSM preserved much of this performance. CONCLUSION: ML models using routinely available noninvasive biomarkers discriminated fibrosis stage and MASH with F2/F3 fibrosis with strong performance, supporting patient selection for clinical trials or practice. Lean predictor models that incorporated LSM outperformed models limited to blood-based biomarkers or a combination of FIB-4 and LSM. These proposed ML models need additional external and prospective validation before deployment for clinical use. CI - Copyright (c) 2026 The Author(s). Published by Wolters Kluwer Health, LLC. on behalf of the American Association for the Study of Liver Diseases. FAU - Loomba, Rohit AU - Loomba R AD - MASLD Research Center, Division of Gastroenterology and Hepatology, University of California at San Diego, La Jolla, California, USA. FAU - Taub, Rebecca AU - Taub R AD - Madrigal Pharmaceuticals, Inc., West Conshohocken, Pennsylvania, USA. FAU - Labriola, Dominic AU - Labriola D AD - Madrigal Pharmaceuticals, Inc., West Conshohocken, Pennsylvania, USA. FAU - Priel, Jan AU - Priel J AD - Cytel, Cambridge, Massachusetts, USA. FAU - Padmanabhan, Krishna AU - Padmanabhan K AD - Madrigal Pharmaceuticals, Inc., West Conshohocken, Pennsylvania, USA. FAU - Kim, W Ray AU - Kim WR AD - Mayo Clinic College of Medicine and Science, Phoenix, Arizona, USA. LA - eng PT - Journal Article DEP - 20261014 PL - United States TA - Hepatol Commun JT - Hepatology communications JID - 101695860 RN - 0 (Biomarkers) SB - IM MH - Humans MH - *Liver Cirrhosis/diagnosis/pathology/etiology MH - Female MH - Predictive Learning Models MH - Biomarkers/blood MH - Retrospective Studies MH - Male MH - *Non-alcoholic Fatty Liver Disease/diagnosis/pathology MH - Middle Aged MH - *Machine Learning MH - Severity of Illness Index MH - Adult MH - Liver/pathology MH - Biopsy MH - Random Forest MH - Predictive Value of Tests PMC - PMC13668301 OTO - NOTNLM OT - MASH OT - biomarker OT - imaging OT - liver fibrosis OT - predictive learning model COIS- Rohit Loomba receives funding support from NCATS (5UL1TR001442), NIDDK (U01DK061734, U01DK130190, and P30DK120515), and the John C. Martin Foundation (RP124) and serves as a consultant to Aardvark Therapeutics, Altimmune, Arrowhead Pharmaceuticals, AstraZeneca, Cascade Pharmaceuticals, Eli Lilly, Gilead, Glympse Bio, Inipharma, Intercept, Inventiva, Ionis, Janssen Inc., Lipidio, Madrigal Pharmaceuticals, Neurobo, Novo Nordisk, Merck, Pfizer, Sagimet, 89 bio, Takeda, Terns Pharmaceuticals, and Viking Therapeutics. In addition, his institution received research grants from Madrigal Pharmaceuticals. Rohit Loomba, Dominic Labriola, and Krishna Padmanabhan are employees and shareholders of Madrigal Pharmaceuticals. Jan Priel has served as a consultant to Madrigal Pharmaceuticals. W. Ray Kim has nothing to report. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/14 CRDT- 2026/10/29 00:06 PHST- 2026/07/15 00:00 [received] PHST- 2026/08/11 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 00:06 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 02009842-202611010-00008 [pii] AID - HEP4-26-1036 [pii] AID - 10.1097/HC9.0000000000001064 [doi] PST - epublish SO - Hepatol Commun. 2026 Oct 14;10(11):e01064. doi: 10.1097/HC9.0000000000001064. eCollection 2026 Nov 1. PMID- 42951894 OWN - NLM STAT- Publisher LR - 20261029 IS - 1530-0293 (Electronic) IS - 0090-3493 (Linking) DP - 2026 Oct 29 TI - Variation in Pediatric Extracorporeal Cardiopulmonary Resuscitation Survival After In-Hospital Cardiac Arrest: Development and Validation of a Survival Model for Risk-Standardized Survival Rates: An Analysis of the Extracorporeal Life Support Organization Registry. LID - 10.1097/CCM.0000000000007383 [doi] AB - OBJECTIVES: To develop and internally validate a multivariable prediction model for survival to hospital discharge after pediatric extracorporeal cardiopulmonary resuscitation (ECPR) for in-hospital cardiac arrest (IHCA), and to use this model to derive risk-standardized survival rates (RSSRs) and quantify between-hospital variation in outcomes. DESIGN: Retrospective multicenter cohort study. SETTING: Hospitals contributing to the Extracorporeal Life Support Organization (ELSO) registry between 2020 and 2023. PATIENTS: Children (< 18 yr) treated with ECPR for IHCA. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: Eligible patients were identified from the ELSO Registry and split into derivation (N = 1060) and validation (N = 317) cohorts using a hospital-stratified split to preserve within-hospital clustering. Survival prediction models were derived using penalized logistic regression with the least absolute shrinkage and selection operator. A full-event model incorporating 14 routinely available clinical variables demonstrated fair discrimination (area under the curve, 0.728 in the derivation cohort and 0.678 in the validation cohort) and good calibration (observed-to-expected ratio, 0.995; Brier score, 0.222 in the validation cohort). This model was embedded in a multilevel logistic regression model to generate hospital-level RSSRs for centers reporting five or more ECPR cases. Between-hospital variation was quantified using the median odds ratio, which was 1.30 (95% CI, 1.11-1.93), indicating significant differences in survival likelihood between centers after adjusting for patient-level risk. CONCLUSIONS: In a large, international cohort of pediatric ECPR patients, we developed and internally validated a clinically interpretable model for survival to hospital discharge. The model performance as a discriminator likely limits its use as a standalone tool for clinical decision-making. However, strong internal calibration allowed for determination of hospital-level RSSRs, which revealed significant inter-hospital variability. These findings demonstrate the feasibility of developing a risk-adjustment model capable of estimating hospital-level RSSRs. Additional external validation and evaluation of residual case-mix differences are needed before such models can be used for benchmarking across pediatric ECPR centers. CI - Copyright (c) 2026 by the Society of Critical Care Medicine and Wolters Kluwer Health, LLC. All Rights Reserved. FAU - Loaec, Morgann AU - Loaec M AD - Division of Critical Care Medicine, Department of Anesthesiology and Critical Care Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA. AD - Resuscitation Science Center, CHOP Research Institute, Children's Hospital of Philadelphia, Philadelphia, PA. FAU - Himebauch, Adam S AU - Himebauch AS AD - Division of Critical Care Medicine, Department of Anesthesiology and Critical Care Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA. AD - Resuscitation Science Center, CHOP Research Institute, Children's Hospital of Philadelphia, Philadelphia, PA. AD - Extracorporeal Membrane Oxygenation Center, Children's Hospital of Philadelphia, Philadelphia, PA. FAU - Balcarcel, Daniel AU - Balcarcel D AD - Division of Critical Care Medicine, Department of Anesthesiology and Critical Care Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA. AD - Resuscitation Science Center, CHOP Research Institute, Children's Hospital of Philadelphia, Philadelphia, PA. FAU - Reilly, John P AU - Reilly JP AD - Division of Pulmonary, Allergy, and Critical Care, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA. AD - Center for Clinical Epidemiology and Biostatistics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA. FAU - Feng, Rui AU - Feng R AD - Center for Clinical Epidemiology and Biostatistics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA. FAU - Regan, Mike AU - Regan M AD - Extracorporeal Membrane Oxygenation Center, Children's Hospital of Philadelphia, Philadelphia, PA. FAU - Thiagarajan, Ravi AU - Thiagarajan R AD - Division of Cardiac Critical Care, Department of Cardiology, Boston Children's Hospital, Boston, MA. FAU - Bembea, Melania M AU - Bembea MM AD - Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, Baltimore, MD. FAU - Lasa, Javier J AU - Lasa JJ AD - Divisions of Cardiology and Critical Care Medicine, Children's Health, UT Southwestern Medical Center, Dallas, TX. FAU - Kilbaugh, Todd J AU - Kilbaugh TJ AD - Division of Critical Care Medicine, Department of Anesthesiology and Critical Care Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA. AD - Resuscitation Science Center, CHOP Research Institute, Children's Hospital of Philadelphia, Philadelphia, PA. AD - Extracorporeal Membrane Oxygenation Center, Children's Hospital of Philadelphia, Philadelphia, PA. FAU - Sutton, Robert M AU - Sutton RM AD - Division of Critical Care Medicine, Department of Anesthesiology and Critical Care Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA. AD - Resuscitation Science Center, CHOP Research Institute, Children's Hospital of Philadelphia, Philadelphia, PA. FAU - Morgan, Ryan W AU - Morgan RW AD - Division of Critical Care Medicine, Department of Anesthesiology and Critical Care Medicine, The Children's Hospital of Philadelphia, Philadelphia, PA. AD - Resuscitation Science Center, CHOP Research Institute, Children's Hospital of Philadelphia, Philadelphia, PA. CN - ELSO Member Centers LA - eng PT - Journal Article DEP - 20261029 PL - United States TA - Crit Care Med JT - Critical care medicine JID - 0355501 SB - IM OTO - NOTNLM OT - extracorporeal cardiopulmonary resuscitation OT - extracorporeal membrane oxygenation systems OT - pediatric in-hospital cardiac arrest OT - prognostication COIS- Drs. Loaec's and Himebauch's institution received funding from the National Heart, Lung, and Blood Institute. Drs. Loaec, Himebauch, and Feng received support for article research from the National Institutes of Health (NIH). Dr. Thiagarajan's institution received funding from the U.S. Department of Defense (DoD) (W81XWH2210301); he received funding from the Society of Critical Care Medicine and Extracorporeal Membrane Oxygenation. Drs. Bembea's, Sutton's, and Morgan's institutions received funding from the NIH. Dr. Bembea's institution received funding from the DoD; she received funding from the U.S. Food and Drug Administration. The remaining authors have disclosed that they do not have any potential conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/29 00:05 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 00:05 [entrez] AID - 00003246-990000000-00992 [pii] AID - 10.1097/CCM.0000000000007383 [doi] PST - aheadofprint SO - Crit Care Med. 2026 Oct 29. doi: 10.1097/CCM.0000000000007383. PMID- 42951888 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Oct 28 TI - A human erythropoietin-albumin fusion protein tailored for enhanced plasma half-life and delivery across mucosal surfaces. LID - 10.1038/s41467-026-78243-z [doi] LID - 10780 AB - Erythropoietin (EPO) products stimulate production of erythrocytes in patients with anemia. The drugs are delivered intravenously or subcutaneously up to several times per week due to short plasma half-life. Improving the pharmacokinetic properties of EPO will allow less frequent administration, and thus impact therapeutic utility and patient compliance. In this study, we took advantage of an albumin variant (QMP) engineered for improved engagement of FcRn, a key regulator of the plasma half-life of albumin. After genetic coupling of EPO to QMP, the fusion protein shows favorable neonatal Fc receptor (FcRn) binding, and 5.2-fold prolonged plasma half-life in human FcRn expressing mice upon intravenous administration, when comparing with EPO fused to WT albumin, with a similar half-life obtained following subcutaneous administration. Importantly, both fusions bind the EPO receptor with binding kinetics equal to that of the product darbepoetin alfa. After intravenous administration, mice receiving darbepoetin alfa experiences a faster drop in EPO levels compared to those given EPO-QMP, and moreover, we measure a prolonged increase in erythrocytes with EPO-QMP. Furthermore, intranasal administration results in 5-fold higher circulating amounts of EPO-QMP and a prolonged increase in erythrocytes compared to darbepoetin alfa. Thus, long-acting EPO-QMP could be an attractive design to explore for both invasive and non-invasive delivery to patients with anemia. CI - (c) 2026. The Author(s). FAU - Anthi, Aina Karen AU - Anthi AK AUID- ORCID: 0000-0002-1725-4571 AD - Precision Immunotherapy Alliance (PRIMA), University of Oslo, Oslo, Norway. AD - Department of Immunology, Oslo University Hospital Rikshospitalet, Oslo, Norway. AD - Department of Pharmacology, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway. FAU - Bejan, Daniela AU - Bejan D AUID- ORCID: 0009-0005-1496-6273 AD - Precision Immunotherapy Alliance (PRIMA), University of Oslo, Oslo, Norway. AD - Department of Immunology, Oslo University Hospital Rikshospitalet, Oslo, Norway. AD - Department of Pharmacology, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway. FAU - Mester, Simone AU - Mester S AD - Precision Immunotherapy Alliance (PRIMA), University of Oslo, Oslo, Norway. AD - Department of Immunology, Oslo University Hospital Rikshospitalet, Oslo, Norway. AD - Department of Pharmacology, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway. FAU - Bethur, Nijaguna AU - Bethur N AD - The Jackson Laboratory, Bar Harbor, ME, USA. FAU - Gonzalo-Gil, Elena AU - Gonzalo-Gil E AD - The Jackson Laboratory, Alachua, FL, USA. FAU - Benjakul, Sopisa AU - Benjakul S AUID- ORCID: 0000-0003-3708-8125 AD - Precision Immunotherapy Alliance (PRIMA), University of Oslo, Oslo, Norway. AD - Department of Immunology, Oslo University Hospital Rikshospitalet, Oslo, Norway. AD - Department of Pharmacology, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway. FAU - Aaen, Kristin Hovden AU - Aaen KH AUID- ORCID: 0000-0001-7181-1052 AD - Precision Immunotherapy Alliance (PRIMA), University of Oslo, Oslo, Norway. AD - Department of Immunology, Oslo University Hospital Rikshospitalet, Oslo, Norway. AD - Department of Pharmacology, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway. FAU - Schlothauer, Tilman AU - Schlothauer T AUID- ORCID: 0000-0001-5844-4438 AD - Biochemical and Analytical Research, Large Molecule Research, Roche Pharma Research and Early Development (pRED), Roche Innovation Center Munich, Penzberg, Germany. FAU - Sandlie, Inger AU - Sandlie I AD - Department of Biosciences, University of Oslo, Oslo, Norway. FAU - Andersen, Jan Terje AU - Andersen JT AUID- ORCID: 0000-0003-1710-1628 AD - Precision Immunotherapy Alliance (PRIMA), University of Oslo, Oslo, Norway. j.t.andersen@medisin.uio.no. AD - Department of Immunology, Oslo University Hospital Rikshospitalet, Oslo, Norway. j.t.andersen@medisin.uio.no. AD - Department of Pharmacology, Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway. j.t.andersen@medisin.uio.no. LA - eng GR - 332727/Norges Forskningsrad (Research Council of Norway)/ GR - 274993/Norges Forskningsrad (Research Council of Norway)/ GR - 287927/Norges Forskningsrad (Research Council of Norway)/ GR - 314909/Norges Forskningsrad (Research Council of Norway)/ GR - 332727/Norges Forskningsrad (Research Council of Norway)/ GR - 314909/Norges Forskningsrad (Research Council of Norway)/ GR - 332727/Norges Forskningsrad (Research Council of Norway)/ GR - 332727/Norges Forskningsrad (Research Council of Norway)/ GR - 332727/Norges Forskningsrad (Research Council of Norway)/ GR - 274993/Norges Forskningsrad (Research Council of Norway)/ GR - 332727/Norges Forskningsrad (Research Council of Norway)/ GR - 287927/Norges Forskningsrad (Research Council of Norway)/ GR - 2019047/Ministry of Health and Care Services | Helse Sor-Ost RHF (Southern and Eastern Norway Regional Health Authority)/ GR - 2019084/Ministry of Health and Care Services | Helse Sor-Ost RHF (Southern and Eastern Norway Regional Health Authority)/ GR - 2024046/Ministry of Health and Care Services | Helse Sor-Ost RHF (Southern and Eastern Norway Regional Health Authority)/ GR - 2019084/Ministry of Health and Care Services | Helse Sor-Ost RHF (Southern and Eastern Norway Regional Health Authority)/ GR - 2024046/Ministry of Health and Care Services | Helse Sor-Ost RHF (Southern and Eastern Norway Regional Health Authority)/ GR - 2019047/Ministry of Health and Care Services | Helse Sor-Ost RHF (Southern and Eastern Norway Regional Health Authority)/ GR - 2024046/Ministry of Health and Care Services | Helse Sor-Ost RHF (Southern and Eastern Norway Regional Health Authority)/ GR - NNF26OC0114113/Novo Nordisk Fonden (Novo Nordisk Foundation)/ PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20261028 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 RN - 11096-26-7 (Erythropoietin) RN - 0 (Recombinant Fusion Proteins) RN - 0 (Receptors, Fc) RN - TW3XAW0RCY (Fc receptor, neonatal) RN - 0 (Albumins) RN - 0 (Histocompatibility Antigens Class I) RN - 15UQ94PT4P (Darbepoetin alfa) RN - 0 (Receptors, Erythropoietin) RN - 0 (EPO protein, human) SB - IM MH - Animals MH - *Erythropoietin/pharmacokinetics/administration & dosage/blood/genetics MH - Humans MH - Half-Life MH - *Recombinant Fusion Proteins/pharmacokinetics/administration & dosage/blood/genetics MH - Receptors, Fc/metabolism/genetics MH - Mice MH - *Albumins/genetics/pharmacokinetics/metabolism/administration & dosage MH - Histocompatibility Antigens Class I/metabolism/genetics MH - Erythrocytes/metabolism/drug effects MH - Darbepoetin alfa/pharmacokinetics/administration & dosage MH - Female MH - Administration, Intranasal MH - Receptors, Erythropoietin/metabolism MH - Protein Binding MH - Administration, Intravenous MH - Male PMC - PMC13716905 COIS- Competing interests: I.S. and J.T.A. are co-inventors on the patent entitled "Albumin Variants and Uses Thereof" (EP3063171B1, US10208102, and US10781245). A.K.A., I.S., and J.T.A. have ownership interests in Superio Bio AS. A.K.A. is employed by Superio Bio AS. S.M., I.S., and J.T.A. have ownership interests in Authera AS. S.M. is employed by Authera AS. The remaining authors declare no competing interests. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/28 CRDT- 2026/10/29 00:03 PHST- 2025/04/07 00:00 [received] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/29 00:03 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 10.1038/s41467-026-78243-z [pii] AID - 78243 [pii] AID - 10.1038/s41467-026-78243-z [doi] PST - epublish SO - Nat Commun. 2026 Oct 28;17(1):10780. doi: 10.1038/s41467-026-78243-z. PMID- 42951879 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261028 IS - 1752-8062 (Electronic) IS - 1752-8054 (Print) IS - 1752-8054 (Linking) VI - 19 IP - 11 DP - 2026 Nov TI - Liquid Compared to Solid Oral Formulations of the Thyroid Hormone Receptor Agonist Elunetirom Under Fasted and Fed Conditions in Healthy Participants. LID - e70743 [pii] LID - 10.1111/cts.70743 [doi] AB - Elunetirom (ABX-002) is a brain-targeted, oral prodrug being developed for the adjunctive treatment of major depressive disorder and bipolar depression. The active moiety, LL-340001, is a thyroid hormone receptor agonist with modest selectivity for beta over alpha thyroid hormone receptors. Upon entering the brain, the intended site of action, elunetirom is hydrolyzed by fatty acid amide hydrolase to LL-340001. In this 3-way randomized, crossover, bioequivalence study, the pharmacokinetics (PK) and safety of an oral liquid formulation used in early clinical development were compared with those of an oral solid formulation intended for later-stage development and potential commercialization, under fasted and fed conditions. Healthy adult participants received single doses of elunetirom (45 mug) as a liquid formulation under fasted conditions and as a solid formulation under fasted conditions and 30 min after consumption of a high-fat meal. Administration of the solid formulation under fasted conditions resulted in elunetirom and LL-340001 exposures similar to those observed with the liquid formulation and is therefore considered to have comparative bioavailability. Food intake increased elunetirom total exposure (AUC) compared with the fasted state but did not affect maximum exposure (C(max)). In contrast, administration with food reduced LL-340001 C(max) compared with the fasted state but did not affect AUC. Food intake delayed the time to reach C(max) (T(max)) for both elunetirom and LL-340001. Elunetirom, administered orally as a liquid and as a solid formulation, was safe and well tolerated under fed and fasted conditions. CI - (c) 2026 Autobahn Therapeutics, Inc. Clinical and Translational Science published by Wiley Periodicals LLC on behalf of American Society for Clinical Pharmacology and Therapeutics. FAU - Harris, Jason R AU - Harris JR AD - Autobahn Therapeutics, San Diego, California, USA. FAU - van Haarst, Aernout D AU - van Haarst AD AUID- ORCID: 0000-0001-6296-2123 AD - Celerion, Lincoln, Nebraska, USA. FAU - Baccei, Jill AU - Baccei J AD - Autobahn Therapeutics, San Diego, California, USA. FAU - Franey, Bridgette AU - Franey B AD - Autobahn Therapeutics, San Diego, California, USA. FAU - Everett, Jason AU - Everett J AD - Autobahn Therapeutics, San Diego, California, USA. FAU - George, Kory AU - George K AD - Autobahn Therapeutics, San Diego, California, USA. FAU - Paglialunga, Sabina AU - Paglialunga S AUID- ORCID: 0000-0002-5666-942X AD - Celerion, Lincoln, Nebraska, USA. FAU - Webster, Kenneth AU - Webster K AD - Celerion, Lincoln, Nebraska, USA. FAU - Hillstrand, Michelle AU - Hillstrand M AD - Autobahn Therapeutics, San Diego, California, USA. FAU - Banfield, Chris AU - Banfield C AD - Autobahn Therapeutics, San Diego, California, USA. AD - CB Pharma Consulting Services LLC, Cambridge, Massachusetts, USA. FAU - Scanlan, Thomas S AU - Scanlan TS AD - Oregon Health & Science University, Portland, Oregon, USA. FAU - Davar, Gudarz AU - Davar G AD - Autobahn Therapeutics, San Diego, California, USA. LA - eng GR - Autobahn Therapeutics/ PT - Comparative Study PT - Journal Article PT - Randomized Controlled Trial PL - United States TA - Clin Transl Sci JT - Clinical and translational science JID - 101474067 RN - 0 (N-(8-(2-hydroxybenzoyl)amino)caprylate) RN - 0 (Prodrugs) RN - 0 (Caprylates) SB - IM MH - Humans MH - Male MH - Adult MH - Cross-Over Studies MH - *Fasting MH - Administration, Oral MH - Female MH - Young Adult MH - Biological Availability MH - Therapeutic Equivalency MH - Healthy Volunteers MH - Area Under Curve MH - Middle Aged MH - Prodrugs/administration & dosage/pharmacokinetics MH - Adolescent MH - *Food-Drug Interactions MH - Caprylates PMC - PMC13716846 OTO - NOTNLM OT - ABX-002 OT - LL-340001 OT - bioavailability OT - bioequivalence OT - bipolar depression OT - major depressive disorder OT - prodrug OT - thyromimetic COIS- A.D.v.H., S.P., and K.W. are employed by Celerion. J.R.H., J.B., B.F., J.E., K.G., M.H., and G.D. are employed by and own stock and/or stock options in Autobahn Therapeutics. T.S.S. is a founder of, advisor to, and owns stock and/or stock options in Autobahn Therapeutics. C.B. is a paid consultant for Autobahn Therapeutics. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:31 PMCR- 2026/10/28 CRDT- 2026/10/28 23:54 PHST- 2026/09/22 00:00 [revised] PHST- 2026/07/07 00:00 [received] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/29 05:31 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:54 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e70743 [pii] AID - CTS70743 [pii] AID - 10.1111/cts.70743 [doi] PST - ppublish SO - Clin Transl Sci. 2026 Nov;19(11):e70743. doi: 10.1111/cts.70743. PMID- 42951877 OWN - NLM STAT- Publisher LR - 20261028 IS - 2328-9503 (Electronic) IS - 2328-9503 (Linking) DP - 2026 Oct 28 TI - Serostatus-Specific Prognostic Profiles and Phenotype-Dependent Therapeutic Windows in Autoimmune Encephalitis: A Multicenter Cohort Study. LID - 10.1002/acn3.70571 [doi] AB - BACKGROUND: While autoimmune encephalitis (AE) outcomes vary, serostatus-specific prognostic determinants and institutional clustering effects remain poorly characterized. We aimed to delineate the distinct prognostic architecture of AE, identifying serostatus-specific risk factors and potential therapeutic windows. METHODS: This multicenter, retrospective cohort study (2016-2024) included 498 patients (382 antibody-positive [Ab+], 116 antibody-negative [Ab-]) across 11 tertiary centers. The primary endpoint was 1-year poor functional outcome (mRS > 2). We utilized Least Absolute Shrinkage and Selection Operator (LASSO) regression and generalized linear mixed models (GLMM) to adjust for institutional clustering, alongside phenotype-treatment interaction analyses. RESULTS: Poor outcomes occurred in 12.9% of patients. GLMM analysis demonstrated negligible institutional heterogeneity. Prognostic architectures diverged by serostatus: Ab+ outcomes were dictated by failure to achieve early functional remission (discharge mRS: OR = 3.74; CASE: OR = 1.56; both p < 0.01) and age >/= 65 years (OR = 5.78, p = 0.040). Conversely, Ab- outcomes were independent of discharge severity metrics, driven by baseline demographic and phenotypic features: age >/= 65 and atypical presentation. Crucially, clinical phenotype modified the therapeutic response (p(interaction) = 0.025). Delayed immunotherapy (>/= 30 days) significantly increased poor outcome risk in atypical presentations (OR = 9.16, p = 0.025) but not in canonical phenotypes. A derived prognostic nomogram yielded excellent discrimination (AUC = 0.909). DISCUSSION: Long-term AE outcomes are dictated by patient-level clinical severity and phenotype, with negligible influence from institutional variations. These findings advocate for a serostatus- and phenotype-stratified management strategy. Crucially, atypical presentations exhibit a highly time-dependent therapeutic window requiring rapid immunotherapy, while elderly patients warrant prioritized intervention to mitigate inherent baseline risks. CI - (c) 2026 The Author(s). Annals of Clinical and Translational Neurology published by Wiley Periodicals LLC on behalf of American Neurological Association. FAU - Tang, Xiaohong AU - Tang X AD - Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. AD - Department of Neurology, People's Hospital of Hongze District, Huaian, China. FAU - Wang, Jingxian AU - Wang J AD - Department of Medical Records, Nanjing Brain Hospital, Nanjing, China. FAU - Zhu, Kaixin AU - Zhu K AD - Department of Neurology, The First Affiliated Hospital of University of Science and Technology of China, Hefei, China. FAU - Zhang, Zhen AU - Zhang Z AD - Department of Neurology, Xuzhou Central Hospital, Xuzhou, China. FAU - Wu, Liao AU - Wu L AD - Department of Neurology, The Affiliated Huai'an No. 1 People's Hospital of Nanjing Medical University, Huai'an, China. FAU - Wang, Wei AU - Wang W AD - Department of Neurology, Yancheng First People's Hospital, Yancheng, China. FAU - Li, Chang AU - Li C AD - Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. FAU - Li, Qingyun AU - Li Q AD - Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. FAU - Li, Hao AU - Li H AD - Department of Neurology, The Fourth Affiliated Hospital of Soochow University, Suzhou, China. FAU - Chen, Jinghua AU - Chen J AD - Department of Oncology, The First Affiliated Hospital of Shandong First Medical University, Jinan, China. FAU - Lian, Zhangxu AU - Lian Z AD - Department of Neurology, Xuzhou Central Hospital, Xuzhou, China. FAU - Liu, Bin AU - Liu B AD - Department of Geriatric Medicine, Nanjing Brain Hospital, Nanjing, China. FAU - Liu, Tengfei AU - Liu T AD - Department of Neurology, Xuzhou Mining Group General Hospital, Xuzhou, China. FAU - Wen, Chunmei AU - Wen C AD - Department of Neurology, Yancheng Third People's Hospital, Yancheng, China. FAU - Wang, Nuan AU - Wang N AD - Department of Neurology, First People's Hospital of Xuzhou, Xuzhou, China. FAU - Qun, Sen AU - Qun S AD - Department of Neurology, The First Affiliated Hospital of University of Science and Technology of China, Hefei, China. AD - Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China. FAU - Xiao, Lishun AU - Xiao L AD - School of Public Health, Xuzhou Medical University, Xuzhou, China. FAU - Lai, Qingwei AU - Lai Q AUID- ORCID: 0000-0001-9805-6894 AD - Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. AD - Department of Neurology, The First Clinical College, Xuzhou Medical University, Xuzhou, China. LA - eng GR - KC25064/Science and Technology Program of Xuzhou/ GR - LCZX202513/Clinical Research Special Project for High Level Hospital Construction in Jiangsu Province/ GR - QL-YB077/XZHMU QL Joint Research Fund/ GR - SHJDBF2024206/Paired Assistance Research Project of The Affiliated Hospital of Xuzhou Medical University/ GR - Special Funding for Overseas Research and Training for Staff of the Affiliated Hospital of Xuzhou Medical University/ PT - Journal Article DEP - 20261028 PL - United States TA - Ann Clin Transl Neurol JT - Annals of clinical and translational neurology JID - 101623278 SB - IM PMC - PMC13716874 OTO - NOTNLM OT - autoimmune encephalitis OT - mixed-effects model OT - prognosis OT - risk stratification OT - seronegative COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 PMCR- 2026/10/28 CRDT- 2026/10/28 23:54 PHST- 2026/09/30 00:00 [revised] PHST- 2026/05/12 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:54 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - ACN370571 [pii] AID - 10.1002/acn3.70571 [doi] PST - aheadofprint SO - Ann Clin Transl Neurol. 2026 Oct 28:10.1002/acn3.70571. doi: 10.1002/acn3.70571. PMID- 42951869 OWN - NLM STAT- Publisher LR - 20261028 IS - 1757-7861 (Electronic) IS - 1757-7853 (Print) IS - 1757-7853 (Linking) DP - 2026 Oct 28 TI - Limited-Incision Kager's Fascia Flap Coverage Versus Open Repair for Acute Achilles Tendon Rupture: A Retrospective Comparative Study. LID - 10.1111/os.70456 [doi] AB - PURPOSE: There is ongoing debate within the medical community regarding the optimal surgical technique for repairing Achilles tendon ruptures. Therefore, this study aimed to compare a novel limited-incision Kager's fascia flap coverage technique with conventional open repair for acute Achilles tendon rupture. METHODS: Ninety patients surgically treated for acute closed Achilles tendon rupture between January 2017 and December 2021 were retrospectively followed, including 41 with limited-incision Kager's fascia flap coverage technique and 49 with traditional open surgical suture. Functional outcomes were evaluated at 12 months postoperatively using the American Orthopaedic Foot and Ankle Society (AOFAS) ankle-hindfoot score and the Achilles Tendon Total Rupture Score (ATRS). Color Doppler ultrasound examination of Achilles tendon blood supply was performed at 3 months postoperatively to assess hemodynamic parameters. To evaluate the influence of age on outcomes, patients were divided into >/= 50 years and < 50 years subgroups. RESULTS: The limited-incision Kager's fascia flap coverage group demonstrated superior outcomes compared with the traditional open surgery group. At 12-month follow-up, the fascia flap group had significantly higher AOFAS scores (91.43 +/- 2.74 vs. 84.80 +/- 2.85) and ATRS scores (94.56 +/- 2.45 vs. 86.26 +/- 3.15) than the control group (both p < 0.001). At 3-month postoperative ultrasound assessment, the fascia flap group also showed better hemodynamic parameters, with higher peak systolic velocity (11.44 +/- 2.06 cm/s vs. 10.39 +/- 1.83 cm/s, p = 0.012), higher end-diastolic velocity (4.45 +/- 0.64 cm/s vs. 3.36 +/- 0.51 cm/s, p < 0.001), and lower resistance index (0.60 +/- 0.06 vs. 0.67 +/- 0.08, p < 0.001) compared with the control group. In age-stratified analyses, the functional and hemodynamic advantages of the fascia flap technique showed consistent trends across both age subgroups, suggesting potential benefits for older patients. All differences were statistically significant (p < 0.05). CONCLUSION: The limited-incision Kager's fascia flap coverage technique for treating acute closed Achilles tendon ruptures shows certain efficacy, improving limb function, reducing surgery-related injuries, and potentially promoting Achilles tendon healing by enhancing local blood supply at the rupture site, thereby improving clinical outcomes and outperforming traditional open surgery. Simultaneously, the technology demonstrates good efficacy across different age groups. CI - (c) 2026 The Author(s). Orthopaedic Surgery published by Tianjin Hospital and John Wiley & Sons Australia, Ltd. FAU - Liu, Changhuan AU - Liu C AD - Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - Hubei Clinical Medical Research Center of Trauma and Microsurgery, Wuhan, Hubei, China. FAU - Wang, Zheng AU - Wang Z AUID- ORCID: 0000-0002-1176-7659 AD - Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - Hubei Clinical Medical Research Center of Trauma and Microsurgery, Wuhan, Hubei, China. AD - Elderly Hip Fracture Diagnosis and Treatment Center, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Fang, Xue AU - Fang X AD - Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - Hubei Clinical Medical Research Center of Trauma and Microsurgery, Wuhan, Hubei, China. AD - Department of Traumatic Orthopedics, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China. FAU - Zhang, Wang AU - Zhang W AUID- ORCID: 0000-0003-2849-1887 AD - Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - Hubei Clinical Medical Research Center of Trauma and Microsurgery, Wuhan, Hubei, China. FAU - Zhu, Lekai AU - Zhu L AUID- ORCID: 0009-0005-2926-8929 AD - Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - Hubei Clinical Medical Research Center of Trauma and Microsurgery, Wuhan, Hubei, China. FAU - Jiang, Haodong AU - Jiang H AD - Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - Hubei Clinical Medical Research Center of Trauma and Microsurgery, Wuhan, Hubei, China. FAU - Tan, Wei AU - Tan W AUID- ORCID: 0009-0009-5937-7367 AD - Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - Hubei Clinical Medical Research Center of Trauma and Microsurgery, Wuhan, Hubei, China. FAU - Zhou, Min AU - Zhou M AD - Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - Hubei Clinical Medical Research Center of Trauma and Microsurgery, Wuhan, Hubei, China. FAU - Li, Zonghuan AU - Li Z AUID- ORCID: 0000-0002-7259-5360 AD - Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - Hubei Clinical Medical Research Center of Trauma and Microsurgery, Wuhan, Hubei, China. FAU - Yang, Xiaqing AU - Yang X AUID- ORCID: 0009-0006-3899-2086 AD - Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - Hubei Clinical Medical Research Center of Trauma and Microsurgery, Wuhan, Hubei, China. FAU - Li, Pengcheng AU - Li P AD - Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - Hubei Clinical Medical Research Center of Trauma and Microsurgery, Wuhan, Hubei, China. AD - Elderly Hip Fracture Diagnosis and Treatment Center, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Chen, Xi AU - Chen X AUID- ORCID: 0000-0003-0763-9598 AD - Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - Hubei Clinical Medical Research Center of Trauma and Microsurgery, Wuhan, Hubei, China. FAU - Luo, Shuilian AU - Luo S AD - Department of Comprehensive Ultrasound, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Xie, Zhe AU - Xie Z AD - Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - Hubei Clinical Medical Research Center of Trauma and Microsurgery, Wuhan, Hubei, China. FAU - Wang, Xin AU - Wang X AUID- ORCID: 0000-0002-7592-7789 AD - Department of Orthopedics Trauma and Microsurgery, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - Hubei Clinical Medical Research Center of Trauma and Microsurgery, Wuhan, Hubei, China. AD - Elderly Hip Fracture Diagnosis and Treatment Center, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. LA - eng GR - YYXKNL2022009/Discipline Construction Project of the Department of Finance of Hubei Province/ PT - Journal Article DEP - 20261028 PL - Australia TA - Orthop Surg JT - Orthopaedic surgery JID - 101501666 SB - IM PMC - PMC13716872 OTO - NOTNLM OT - Achilles tendon rupture OT - Kager's fascia flap OT - color Doppler ultrasonography OT - hemodynamics OT - limited incision repair OT - minimally invasive surgery COIS- The authors declare no conflicts of interest. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 PMCR- 2026/10/28 CRDT- 2026/10/28 23:43 PHST- 2026/09/26 00:00 [revised] PHST- 2026/07/06 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:43 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - OS70456 [pii] AID - 10.1111/os.70456 [doi] PST - aheadofprint SO - Orthop Surg. 2026 Oct 28:10.1111/os.70456. doi: 10.1111/os.70456. PMID- 42951866 OWN - NLM STAT- Publisher LR - 20261028 IS - 1532-6535 (Electronic) IS - 0009-9236 (Print) IS - 0009-9236 (Linking) DP - 2026 Oct 28 TI - Evaluating the Association Between PPI Use and Dementia Risk Considering Methodological Choices: An Updated Meta-Analysis. LID - 10.1002/cpt.70534 [doi] AB - Proton pump inhibitors (PPIs), commonly prescribed for gastrointestinal disorders, have been investigated for their association with the risk of dementia. We aimed to assess the association between PPI use and dementia risk in older adults and examine the influence of methodological choices on this association. We conducted a meta-analysis of longitudinal observational studies, including subgroup analyses to address time-related biases and comparator selection. Relevant studies were systematically searched in the MEDLINE, Embase, and CENTRAL databases from their inception to January 25, 2026. Twenty-three studies of older adults without dementia at baseline, reporting adjusted estimates for PPI users versus a comparator group, met the inclusion criteria. The pooled hazard ratio (HR) indicated a modest association between PPI use and dementia (HR = 1.18, 95% confidence interval [CI] 1.02-1.37). Dementia risk did not differ significantly between PPI users and users of histamine-2 receptor antagonists (H2RAs) (HR = 1.04, 95% CI 0.89-1.20). In a post hoc analysis, H2RA use itself was associated with dementia (HR = 1.44, 95% CI 1.07-1.93), whereas excluding H2RA-treated patients strengthened the PPI estimate (HR = 1.75, 95% CI 1.38-2.22). Estimates also differed according to how time-related bias was handled: lag time, HR = 1.25, 95% CI 1.07-1.46; time-varying exposure, HR = 1.38, 95% CI 1.16-1.64. PPI use was modestly associated with incident dementia. However, the strength and specificity depended substantially on comparator selection and handling of time-related bias. Well-designed, long-term studies using active comparators are needed to clarify whether this effect is PPI-specific. CI - (c) 2026 The Author(s). Clinical Pharmacology & Therapeutics (c) 2026 American Society for Clinical Pharmacology and Therapeutics. FAU - Ha, Heonyoung AU - Ha H AUID- ORCID: 0009-0007-9739-0218 AD - Graduate Program of Industrial Pharmaceutical Sciences, Yonsei University, Incheon, Republic of Korea. FAU - Lee, Hyun Woo AU - Lee HW AUID- ORCID: 0000-0002-5676-3846 AD - Department of Pharmaceutical Medicine and Regulatory Science, Colleges of Medicine and Pharmacy, Yonsei University, Incheon, Republic of Korea. AD - Department of Pharmacy and Yonsei Institute of Pharmaceutical Sciences, College of Pharmacy, Yonsei University, Incheon, Republic of Korea. FAU - Park, Jae-Eun AU - Park JE AUID- ORCID: 0009-0003-6528-4585 AD - College of Pharmacy, Yeungnam University, Gyeongsan, Republic of Korea. FAU - Ye, Byoung Seok AU - Ye BS AUID- ORCID: 0000-0003-0187-8440 AD - Department of Neurology, College of Medicine, Yonsei University, Seoul, Republic of Korea. AD - Metabolism-Dementia Research Institute, Yonsei University College of Medicine, Seoul, Republic of Korea. FAU - Yu, Yun Mi AU - Yu YM AUID- ORCID: 0000-0002-8267-9453 AD - Department of Pharmaceutical Medicine and Regulatory Science, Colleges of Medicine and Pharmacy, Yonsei University, Incheon, Republic of Korea. AD - Department of Pharmacy and Yonsei Institute of Pharmaceutical Sciences, College of Pharmacy, Yonsei University, Incheon, Republic of Korea. AD - Yonsei-SL Bigen Research Institute, College of Pharmacy, Yonsei University, Incheon, Republic of Korea. FAU - Ah, Young-Mi AU - Ah YM AUID- ORCID: 0000-0003-1607-9828 AD - College of Pharmacy, Yeungnam University, Gyeongsan, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Clin Pharmacol Ther JT - Clinical pharmacology and therapeutics JID - 0372741 SB - IM PMC - PMC13716873 COIS- The authors declared no competing interests for this work. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 PMCR- 2026/10/28 CRDT- 2026/10/28 23:43 PHST- 2026/04/10 00:00 [received] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:43 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - CPT70534 [pii] AID - 10.1002/cpt.70534 [doi] PST - aheadofprint SO - Clin Pharmacol Ther. 2026 Oct 28:10.1002/cpt.70534. doi: 10.1002/cpt.70534. PMID- 42951862 OWN - NLM STAT- Publisher LR - 20261028 IS - 1941-2444 (Electronic) IS - 0148-6071 (Linking) DP - 2026 Oct 28 TI - Malnutrition severity and nutrition care in hospitalized adults: A descriptive cohort study. LID - 10.1002/jpen.70164 [doi] AB - BACKGROUND: Malnutrition is prevalent in acute hospital populations. Morrison Healthcare launched a data system enabling hospitals to track and report malnutrition, including dietitian diagnosis, assigned ICD-10 codes, nutrition interventions, and length of stay. This study aimed to describe characteristics of adults with malnutrition and examine associations between nutrition care and hospital length of stay. METHODS: This descriptive cohort study analyzed de-identified data from hospitalized adults with malnutrition identified by a dietitian and/or ICD-10 coded at discharge between December 2019 and January 2025. Categorical variables are reported as frequencies (%) and continuous variables as means +/- SD. Chi-square tests assessed associations between malnutrition diagnosis and patient characteristics. Regression was used to examine associations between patient characteristics, nutrition interventions, and hospital length of stay. RESULTS: Among patients with malnutrition identified by either source, dietitians diagnosed severe malnutrition in 102,484 (57.2%) and mild/moderate malnutrition in 50,780 (28.4%). Malnutrition was identified by dietitians in a higher proportion of patients than was documented using ICD-10 codes (85.6% vs. 76.1%, P < 0.001). Mean length of stay was 9.6 (11.7) days and was significantly different between malnutrition diagnosis groups. Early assessment by a dietitian within 24 h was associated with ~31% shorter length of stay. Length of stay was shorter in patients with malnutrition who received oral or enteral nutrition support. CONCLUSION: Differences between dietitian-identified malnutrition and ICD-10 coded malnutrition highlight variability in documentation practices. Earlier dietitian assessment and selected nutrition interventions were associated with differences in hospital length of stay. Further research is needed to evaluate causal relationships and long-term impacts of nutrition care. CI - (c) 2026 The Author(s). Journal of Parenteral and Enteral Nutrition published by Wiley Periodicals LLC on behalf of American Society for Parenteral and Enteral Nutrition. FAU - Rozga, Mary AU - Rozga M AD - Altarum, Novi, Michigan, USA. FAU - Oblein, Julia AU - Oblein J AD - Morrison Healthcare, Atlanta, Georgia, USA. FAU - Leger, Gisele AU - Leger G AUID- ORCID: 0000-0002-0205-5246 AD - Morrison Healthcare, Atlanta, Georgia, USA. FAU - Janowski, Maureen AU - Janowski M AUID- ORCID: 0000-0002-3758-4893 AD - Morrison Healthcare, Atlanta, Georgia, USA. FAU - Papoutsakis, Constantina AU - Papoutsakis C AUID- ORCID: 0000-0001-7173-275X AD - Academy of Nutrition and Dietetics, Chicago, Illinois, USA. FAU - Steiber, Alison AU - Steiber A AD - Academy of Nutrition and Dietetics, Chicago, Illinois, USA. FAU - Handu, Deepa AU - Handu D AUID- ORCID: 0000-0002-2547-147X AD - Academy of Nutrition and Dietetics, Chicago, Illinois, USA. AD - Evidence Analysis Center, Chicago, Illinois, USA. LA - eng GR - This research was funded by Morrison Healthcare/ PT - Journal Article DEP - 20261028 PL - United States TA - JPEN J Parenter Enteral Nutr JT - JPEN. Journal of parenteral and enteral nutrition JID - 7804134 SB - IM OTO - NOTNLM OT - ICD codes OT - length of stay OT - malnutrition OT - nutrition assessment OT - registered dietitian nutritionist EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 23:33 PHST- 2026/10/05 00:00 [revised] PHST- 2025/12/05 00:00 [received] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:33 [entrez] AID - 10.1002/jpen.70164 [doi] PST - aheadofprint SO - JPEN J Parenter Enteral Nutr. 2026 Oct 28. doi: 10.1002/jpen.70164. PMID- 42951853 OWN - NLM STAT- Publisher LR - 20261028 IS - 0529-5815 (Print) IS - 0529-5815 (Linking) VI - 64 IP - 12 DP - 2026 Oct 29 TI - [Preoperative serum thyroglobulin in papillary thyroid carcinoma: associations with tumor burden and cervical lymph node metastasis]. PG - 1307-1315 LID - 10.3760/cma.j.cn112139-20260618-00268 [doi] AB - Objectives: To investigate the correlation between preoperative serum thyroglobulin(Tg) levels and papillary thyroid carcinoma(PTC) tumor burden, and to evaluate Tg's diagnostic value for cervical lymph node metastasis in PTC. Methods: This is a retrospective case series study. The clinicopathological data of 1 585 patients with PTC who underwent initial surgical treatment at the Department of General Surgery, Peking Union Medical College Hospital between November 2013 and December 2020 were retrospectively analyzed. There were 563 male and 1 022 female patients, aged (41.1+/-10.5)years (range: 15 to 74 years). All included patients were thyroglobulin antibody(TgAb)-negative preoperatively and had no concomitant benign thyroid disease. A stratified multivariate linear regression model was used to analyze the weight of different tumor burden dimensions in explaining log-transformed Tg (LnTg). Block-specific analysis was used to evaluate the independent explanatory contribution of each dimension to LnTg. Restricted cubic spline (RCS) analysis was performed to assess both overall and nonlinear associations between tumor burden parameters and LnTg. Receiver operating characteristic (ROC) curves were constructed to determine the optimal cutoff value of preoperative Tg for predicting lateral cervical lymph node metastasis, and its diagnostic performance was evaluated both alone and in combination with preoperative ultrasound findings. Results: Univariate analysis showed statistically significant differences in serum Tg levels among patients with different tumor numbers (1 vs. 2 vs. >/=3), diffrent maximum tumor diameter (1-2 cm vs. >2 cm), different degrees of capsular invasion (no invasion vs. capsular invasion only vs. extraglandular invasion), different total numbers of metastatic lymph nodes (0 vs. 1-5 vs. >/=6), and with or without central or lateral cervical lymph node metastasis (all P<0.01). Stratified regression analysis showed that primary tumor burden contributed most to the independent explanation of Tg (partial R(2)=0.130, P<0.01), followed by lymph node burden (partial R(2)=0.029, P<0.01). Restricted cubic spline analysis confirmed that maximum tumor diameter, total number of metastatic lymph nodes and number of metastatic lateral cervical lymph nodes were correlated with LnTg (overall effect P<0.01), and the former two showed no clear nonlinear trend. The area under the receiver operating characteristic curve of preoperative Tg for predicting lateral neck lymph node metastasis was 0.742 (95%CI: 0.699 to 0.782), with an optimal cutoff value of 23.8 ng/ml (Roche). At this cutoff value, the negative predictive value (NPV) for predicting lateral cervical lymph node metastasis was 93.4%, slightly lower than that of ultrasound (97.5%). When combined with preoperative ultrasound, the positive predictive value (PPV) reached 94.0%, while a favorable NPV (93.5%) was maintained. Its NPV for predicting extensive lymph node metastasis (>/=6 nodes) was 89.4%, slightly lower than that of ultrasound (91.5%). Conclusions: In PTC patients with negative TgAb and no concomitant benign thyroid diseases, preoperative serum Tg levels are correlated with tumor burden indicators such as the maximum tumor diameter and the number of metastatic lymph nodes. When 23.8 ng/ml (Roche assay) is adopted as the cutoff value, preoperative serum Tg shows a high NPV for predicting lateral cervical and extensive lymph node metastasis. Moreover, preoperative Tg can improve the PPV of ultrasound for predicting lateral cervical lymph node metastasis, thereby assisting preoperative ultrasonographic evaluation of lateral cervical lymph node metastasis. FAU - Huang, H AU - Huang H AD - Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing 100730, China. FAU - Ge, G N AU - Ge GN AD - International Medical Department, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing 100730, China. FAU - Kuang, Z F AU - Kuang ZF AD - Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing 100730, China. FAU - Zhang, C J AU - Zhang CJ AD - Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing 100730, China. FAU - Chen, G AU - Chen G AD - Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing 100730, China. FAU - Xu, X Q AU - Xu XQ AD - Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing 100730, China. FAU - Cao, Y AU - Cao Y AD - Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing 100730, China. FAU - Zhao, Y AU - Zhao Y AD - Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing 100730, China. FAU - Yao, J J AU - Yao JJ AD - Department of Anesthesiology and Operating Room, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing 100730, China. FAU - Xia, Y AU - Xia Y AD - Department of Ultrasound, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China. FAU - Li, X Y AU - Li XY AD - Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing 100730, China. LA - chi PT - English Abstract PT - Journal Article DEP - 20261029 PL - China TA - Zhonghua Wai Ke Za Zhi JT - Zhonghua wai ke za zhi [Chinese journal of surgery] JID - 0153611 SB - IM COIS- 所有作者声明不存在利益冲突 EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 23:31 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:31 [entrez] AID - 10.3760/cma.j.cn112139-20260618-00268 [doi] PST - aheadofprint SO - Zhonghua Wai Ke Za Zhi. 2026 Oct 29;64(12):1307-1315. doi: 10.3760/cma.j.cn112139-20260618-00268. PMID- 42951850 OWN - NLM STAT- Publisher LR - 20261028 IS - 0529-5815 (Print) IS - 0529-5815 (Linking) VI - 64 IP - 12 DP - 2026 Oct 29 TI - [Analysis of surgical outcomes in 170 patients with parathyroid carcinoma: a single institute experience]. PG - 1284-1292 LID - 10.3760/cma.j.cn112139-20260531-00236 [doi] AB - Objective: To investigate postoperative survival, recurrence, metastasis, and risk factors in patients with parathyroid carcinoma (PC). Methods: This is a retrospective case series study. Clinicopathological data from 170 PC patients treated at Beijing Chao-Yang Hospital, Capital Medical University, between January 2010 and December 2025 were retrospectively analyzed. There were 85 male patients and 85 female patients, with an age of (49.3+/-14.7) years (range:19 to 80 years). Among them, 63 patients underwent initial surgery (usually extrafascial dissection) at our institution, and 107 patients received supplemental reoperations (usually extended en bloc resection) due to tumor recurrence, metastasis, or positive surgical margins after previous surgery at other institutions. Postoperative overall survival (OS) of patients with PC was analyzed using the Kaplan-Meier method. The cumulative incidence of distant metastasis (DM) was estimated using the cumulative incidence function (CIF), and the Fine-Gray competing risk model was applied for multivariate analysis to identify independent risk factors for DM. Results: The follow-up period (M(IQR)) was 3.7 (4.1) years (range: 0.5 to 13.9 years) after surgery. The expected 5-year and 10-year OS rates for the whole cohort were 80.4% and 73.4%, respectively. Among patients who underwent initial surgery at Beijing Chao-Yang Hospital, Capital Medical University, 92.1% (58/63) achieved biochemical remission, 3.2% (2/63) survived with cancer, and 4.7% (3/63) died of disease. Among patients with recurrent disease after previous operations at other institutions, 26.5% (18/68) achieved biochemical remission, 35.3% (24/68) survived with cancer, and 38.2% (26/68) died of disease. Among patients who received supplemental reoperations due to positive surgical margins, the biochemical remission rate was 97.4% (38/39), while the tumor-bearing survival rate was 2.6% (1/39); no deaths occurred. Patients with DM suffered a worse 5-year OS rate than those without DM (43.5% vs. 96.0%, P<0.01). Upper aerodigestive tract (UAT) invasion, local recurrence, and lymph node metastasis were also associated with decreased OS (all P<0.01). In contrast, patients who received R0 resection achieved significantly higher 5-year OS rate than those with non-R0 resection (91.3% vs. 44.8%, P<0.01). Multivariate Fine-Gray model analysis revealed that non-R0 resection (sHR=4.701, 95%CI: 1.128 to 19.593, P=0.034), UAT invasion (sHR=2.217, 95%CI: 1.075 to 4.574, P=0.031), lymph node metastasis (sHR=1.977, 95%CI: 1.022 to 3.827, P=0.043), and higher preoperative parathyroid hormone (PTH) levels (sHR=1.000, 95%CI: 1.000 to 1.001, P=0.016) were independent risk factors of DM. Conclusions: Non-R0 resection, UAT invasion, lymph node metastasis, and higher preoperative PTH levels are independent risk factors for possible DM involvement in patients with PC. Extrafascial dissection and extended en bloc resection might respectively improve the biochemical remission rate for the initial surgery and the local control rate for local recurrent PC. FAU - Zhao, T AU - Zhao T AD - Division of Thyroid and Neck Surgery, Department of General Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China. FAU - Shen, H AU - Shen H AD - Division of Thyroid and Neck Surgery, Department of General Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China. FAU - Wei, B B AU - Wei BB AD - Division of Thyroid and Neck Surgery, Department of General Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China. FAU - Huang, N AU - Huang N AD - Division of Thyroid and Neck Surgery, Department of General Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China. FAU - Shen, R F AU - Shen RF AD - Division of Thyroid and Neck Surgery, Department of General Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China. FAU - Liu, X AU - Liu X AD - Division of Thyroid and Neck Surgery, Department of General Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China. FAU - Wang, Q AU - Wang Q AD - Division of Thyroid and Neck Surgery, Department of General Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China. FAU - Wang, J C AU - Wang JC AD - Division of Thyroid and Neck Surgery, Department of General Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China. FAU - Feng, D L AU - Feng DL AD - Division of Thyroid and Neck Surgery, Department of General Surgery, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China. LA - chi GR - PX2023009/Beijing Municipal Administration of Hospitals Scientific Research Incubating Program/ PT - English Abstract PT - Journal Article DEP - 20261029 PL - China TA - Zhonghua Wai Ke Za Zhi JT - Zhonghua wai ke za zhi [Chinese journal of surgery] JID - 0153611 SB - IM COIS- 所有作者声明不存在利益冲突 EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 23:31 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:31 [entrez] AID - 10.3760/cma.j.cn112139-20260531-00236 [doi] PST - aheadofprint SO - Zhonghua Wai Ke Za Zhi. 2026 Oct 29;64(12):1284-1292. doi: 10.3760/cma.j.cn112139-20260531-00236. PMID- 42951849 OWN - NLM STAT- Publisher LR - 20261028 IS - 0529-5815 (Print) IS - 0529-5815 (Linking) VI - 64 IP - 12 DP - 2026 Oct 29 TI - [A clinical study on predicting the surgical difficulty of robot-assisted total thyroidectomy combined with lateral cervical lymph node dissection based on machine learning algorithms]. PG - 1276-1283 LID - 10.3760/cma.j.cn112139-20260402-00140 [doi] AB - Objectives: To construct a prediction model based on machine learning algorithms for the surgical difficulty of robot-assisted total thyroidectomy combined with lateral cervical lymph node dissection,and to validate its predictive performance and clinical utility. Methods: This retrospective case series study collected clinical data from 496 patients with differentiated thyroid cancer admitted to the Thyroid and Breast Surgery Department of the 960(th) Hospital of the People's Liberation Army Joint Logistics Support Force between January 2014 and July 2024, who underwent robot-assisted total thyroidectomy combined with lateral neck lymph node dissection using the Da Vinci system. There were 143 male cases and 353 female cases,aged (36.5+/-10.4)years (range: 18 to 70 years). Patients were randomly divided into a training set (n=347) and a validation set (n=149) at a ratio of 7ratio3. Clinical characteristics,ultrasound features,and hematological parameters were collected. Feature selection was performed using the least absolute shrinkage and selection operator (LASSO) regression. Based on the selected features,eight machine learning algorithms-including logistic regression,support vector machine (SVM),gradient boosting machine,neural network,random forest,extreme gradient boosting (XGBoost),k-nearest neighbors and adaptive boosting-were utilized to construct prediction models. Model performance was evaluated using metrics such as the area under the receiver operating characteristic curve (AUC) and F1 score. The Shapley additive explanations (SHAP) method was applied to conduct an interpretable analysis of the optimal model. Results: A total of 8 key features for predicting surgical difficulty were selected through LASSO regression dimensionality reduction: the maximum short-axis diameter of positive lateral cervical lymph nodes,positive lymph nodes in cervical levels Ⅱ and Ⅲ,the number of positive lateral cervical lymph nodes,sex,diffuse thyroid changes on ultrasound,weight,surgeon's years of experience,and thyroid peroxidase antibody (TPOAb) positivity. The SVM model demonstrated the best performance,with the highest AUC (0.815),the highest F1 score (0.708), and a relatively high sensitivity (0.727). SHAP analysis confirmed that lateral cervical lymph node characteristics,higher body weight,male sex,diffuse thyroid changes on ultrasound,and TPOAb positivity were high-risk factors associated with increased surgical difficulty. Conclusions: The model constructed based on patients' preoperative multidimensional features exhibits good predictive performance for the surgical difficulty of Da Vinci robot-assisted total thyroidectomy combined with lateral cervical lymph node dissection. The integration of SHAP interpretable analysis enables an individualized and visualized preoperative risk assessment,which provides a scientific basis for surgeons to rationally allocate medical resources and optimize perioperative management. FAU - Li, W T AU - Li WT AD - Department of Thyroid and Breast Surgery,the 960th Hospital of the People's Liberation Army Joint Logistics Support Force, Jinan 250117, China. FAU - He, Q Q AU - He QQ AD - Department of Thyroid and Breast Surgery,the 960th Hospital of the People's Liberation Army Joint Logistics Support Force, Jinan 250117, China. FAU - Ma, Y H AU - Ma YH AD - Department of Thyroid and Breast Surgery,the 960th Hospital of the People's Liberation Army Joint Logistics Support Force, Jinan 250117, China. FAU - Song, T Y AU - Song TY AD - Department of Thyroid and Breast Surgery,the 960th Hospital of the People's Liberation Army Joint Logistics Support Force, Jinan 250117, China. FAU - Li, Z H AU - Li ZH AD - Department of Thyroid and Breast Surgery,the 960th Hospital of the People's Liberation Army Joint Logistics Support Force, Jinan 250117, China. FAU - Pan, B X AU - Pan BX AD - Department of Thyroid and Breast Surgery,the 960th Hospital of the People's Liberation Army Joint Logistics Support Force, Jinan 250117, China. FAU - Li, J X AU - Li JX AD - Department of Thyroid and Breast Surgery,the 960th Hospital of the People's Liberation Army Joint Logistics Support Force, Jinan 250117, China. FAU - Zhou, P AU - Zhou P AD - Department of Thyroid and Breast Surgery,the 960th Hospital of the People's Liberation Army Joint Logistics Support Force, Jinan 250117, China. LA - chi GR - 202430064/Jinan Clinical Medical Science and Technology Innovation Program/ PT - English Abstract PT - Journal Article DEP - 20261029 PL - China TA - Zhonghua Wai Ke Za Zhi JT - Zhonghua wai ke za zhi [Chinese journal of surgery] JID - 0153611 SB - IM COIS- 所有作者声明不存在利益冲突 EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 23:31 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:31 [entrez] AID - 10.3760/cma.j.cn112139-20260402-00140 [doi] PST - aheadofprint SO - Zhonghua Wai Ke Za Zhi. 2026 Oct 29;64(12):1276-1283. doi: 10.3760/cma.j.cn112139-20260402-00140. PMID- 42951846 OWN - NLM STAT- Publisher LR - 20261028 IS - 0529-5815 (Print) IS - 0529-5815 (Linking) VI - 64 IP - 12 DP - 2026 Oct 29 TI - [Research report on the surgical recommendations of the "2025 American Thyroid Association management guidelines for adult patients with differentiated thyroid cancer"]. PG - 1250-1259 LID - 10.3760/cma.j.cn112139-20260714-00313 [doi] AB - The "2025 American Thyroid Association management guidelines for adult patients with differentiated thyroid cancer" released in 2025 proposed several new recommendations regarding the assessment of thyroid nodules, surgical treatment options for differentiated thyroid cancer, and postoperative management. However, some of the recommendations were based on relatively low-level evidence and failed to fully reflect the epidemiological characteristics, the distribution of medical resources, and clinical practice variations of thyroid cancer in China. Therefore, the General Surgery Branch of China International Exchange and Promotive Association for Medical and Health Care and other academic organizations, focusing on seven surgical management issues including the indications for fine-needle aspiration biopsy of thyroid nodules, whether low-risk papillary thyroid cancer can be selected for active surveillance, the indications for unilateral thyroid lobectomy, prophylactic central compartment lymph node dissection, whether papillary thyroid cancer can be treated with ablation therapy, the placement of drainage tubes after thyroid cancer surgery, and targets for thyroid stimulating hormone suppression therapy, based on the latest international and domestic evidence-based medicine evidence and Chinese clinical practice, organized experts in relevant fields to conduct multiple rounds of discussion and voting, thereby developing this research report. It follows the principles of individualization and standardization, aiming to provide a reference for maximizing patient benefit. CN - General Surgery Branch of China International Exchange and Promotive Association for Medical and Health Care CN - Expert Working Group of Thyroid Association, Chinese College of Surgeons, Chinese Medical Doctor Association CN - Thyroid Disease Committee of Chinese Research Hospital Association CN - Thyroid and Metabolism Surgery Group of the Chinese Society of Surgery LA - chi PT - English Abstract PT - Journal Article DEP - 20261029 PL - China TA - Zhonghua Wai Ke Za Zhi JT - Zhonghua wai ke za zhi [Chinese journal of surgery] JID - 0153611 SB - IM COIS- 所有编者声明不存在利益冲突 EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 23:31 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:31 [entrez] AID - 10.3760/cma.j.cn112139-20260714-00313 [doi] PST - aheadofprint SO - Zhonghua Wai Ke Za Zhi. 2026 Oct 29;64(12):1250-1259. doi: 10.3760/cma.j.cn112139-20260714-00313. PMID- 42951844 OWN - NLM STAT- Publisher LR - 20261028 IS - 0529-5815 (Print) IS - 0529-5815 (Linking) VI - 64 IP - 12 DP - 2026 Oct 29 TI - [Evolution of surgical strategies for secondary hyperparathyroidism]. PG - 1238-1243 LID - 10.3760/cma.j.cn112139-20260521-00210 [doi] AB - Secondary hyperparathyroidism (SHPT) is an important component of chronic kidney disease-mineral and bone disorder (CKD-MBD). It may be accompanied by bone pain, pruritus, abnormalities in bone metabolism, and vascular and soft-tissue calcifications, thereby increasing the disease burden in patients with end-stage kidney disease. Historically, surgical management of SHPT was mainly based on persistently elevated parathyroid hormone (PTH) levels and patients' responses to medical therapy, gradually establishing a management strategy centered on PTH control. This strategy has played an important role in selecting candidates for surgery and facilitating multidisciplinary communication. With the evolution of the CKD-MBD concept and accumulating evidence on clinical outcomes, the goals of surgical treatment have gradually expanded from merely lowering PTH levels to improving bone metabolism, relieving symptoms, controlling CKD-MBD-related target-organ damage, and improving long-term outcomes. Based on current guidelines, expert consensus, and clinical studies, this review discusses the historical basis and limitations of PTH threshold-based strategies and examines the evolution of surgical management for SHPT, with particular emphasis on the timing of surgical evaluation, surgical indications, choice of surgical procedure, perioperative management, postoperative follow-up, and future directions for optimization. FAU - Zheng, X AU - Zheng X AD - Department of Thyroid Surgery, West China Hospital, Sichuan University, Chengdu 610041, China. FAU - Zhou, L AU - Zhou L AD - Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China. FAU - Ou, X H AU - Ou XH AD - Department of Nuclear Medicine, West China Hospital, Sichuan University, Chengdu 610041, China. FAU - Wang, Q AU - Wang Q AD - Department of Endocrinology and Metabolism, West China Hospital, Sichuan University, Chengdu 610041, China. FAU - Shi, Y Y AU - Shi YY AD - Department of Nephrology, West China Hospital, Sichuan University, Chengdu 610041, China. FAU - Li, Z H AU - Li ZH AD - Department of Thyroid Surgery, West China Hospital, Sichuan University, Chengdu 610041, China. LA - chi GR - 82570926/National Natural Science Foundation of China (General Program)/ PT - English Abstract PT - Journal Article DEP - 20261029 PL - China TA - Zhonghua Wai Ke Za Zhi JT - Zhonghua wai ke za zhi [Chinese journal of surgery] JID - 0153611 SB - IM COIS- 所有作者声明不存在利益冲突 EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 23:31 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:31 [entrez] AID - 10.3760/cma.j.cn112139-20260521-00210 [doi] PST - aheadofprint SO - Zhonghua Wai Ke Za Zhi. 2026 Oct 29;64(12):1238-1243. doi: 10.3760/cma.j.cn112139-20260521-00210. PMID- 42951843 OWN - NLM STAT- Publisher LR - 20261028 IS - 0529-5815 (Print) IS - 0529-5815 (Linking) VI - 64 IP - 12 DP - 2026 Oct 29 TI - [Advances in the diagnosis and treatment of primary hyperparathyroidism and the evolution of its clinical characteristics in China]. PG - 1233-1237 LID - 10.3760/cma.j.cn112139-20260601-00240 [doi] AB - Primary hyperparathyroidism (PHPT) is a common clinical endocrine and metabolic disorder. Its clinical phenotype spectrum is undergoing a profound shift, evolving from the classic, severe presentations characterized by bone and kidney involvement towards more occult and subclinical forms. With the increasing adoption of routine biochemical screening and advancements in diagnostic technologies, there has been a notable rise in subclinical phenotypes of PHPT in China, particularly asymptomatic PHPT and normocalcemic PHPT. This trend not only reflects the progress of Chinese public health system but also imposes higher demands for precision in clinical diagnosis and treatment. Meanwhile, preoperative localization techniques have evolved from traditional ultrasound and radionuclide scanning to the era of four-dimensional CT and choline positron emission tomography, laying a solid foundation for the widespread adoption of minimally invasive parathyroidectomy. However, the clinical management of PHPT in China still faces significant challenges, including insufficient awareness among primary care physicians, imbalanced accessibility to advanced imaging equipment, and the complexity of managing multi-gland disease. This review systematically examines relevant literature published both domestically and internationally in recent years. It aims to outline the theoretical evolution, technological advancements, and disease characteristics specific to the Chinese population, with the goal of providing a reference for the standardized diagnosis and treatment of PHPT in China. FAU - Gao, J Y AU - Gao JY AD - Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China. FAU - Liao, Q AU - Liao Q AD - Department of General Surgery, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China. LA - chi GR - 2025‑PUMCH‑H‑008/National High Level Hospital Clinical Research Funding/ PT - English Abstract PT - Journal Article DEP - 20261029 PL - China TA - Zhonghua Wai Ke Za Zhi JT - Zhonghua wai ke za zhi [Chinese journal of surgery] JID - 0153611 SB - IM COIS- 所有作者声明不存在利益冲突 EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 23:31 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:31 [entrez] AID - 10.3760/cma.j.cn112139-20260601-00240 [doi] PST - aheadofprint SO - Zhonghua Wai Ke Za Zhi. 2026 Oct 29;64(12):1233-1237. doi: 10.3760/cma.j.cn112139-20260601-00240. PMID- 42951836 OWN - NLM STAT- Publisher LR - 20261028 IS - 1540-8159 (Electronic) IS - 0147-8389 (Linking) DP - 2026 Oct 28 TI - Feasibility of Left Bundle Branch Pacing and Management of Jailed Leads After Transcatheter Tricuspid Valve Implantation. LID - 10.1111/pace.70464 [doi] AB - Atrioventricular conduction disturbances and the need for cardiac implantable electronic device (CIED) therapy are frequently required after transcatheter tricuspid valve implantation (TTVI). The interaction between the annulus, transcatheter heart valve and the leads is often complex and the optimal management under debate. We prospectively included 36 patients undergoing TTVI between June 2021 and January 2025. 14 patients had a preexisting CIED. 11 patients experienced right ventricular lead-jailing without signs for early or delayed lead failure after one year, one patient underwent lead extraction and two patients had a leadless pacemaker. In four patients, a pacemaker in LBBAP was successfully implanted due to new-onset postoperative atrioventricular block. The device measurements as well as the valve function remained stable over a follow-up of at least one up to 2.5 years with persistent LBB-capture. This case series demonstrates that LBBAP following TTVI is feasible with encouraging early outcomes. Careful patient selection and dedicated follow-up are essential for patients with CIED treated with TTVI. CI - (c) 2026 The Author(s). Pacing and Clinical Electrophysiology published by Wiley Periodicals LLC. FAU - Seiler, Thomas AU - Seiler T AUID- ORCID: 0000-0001-8325-2075 AD - Department of Cardiology, Inselspital, University Hospital Bern, University of Bern, Bern, Switzerland. FAU - Haeberlin, Andreas AU - Haeberlin A AUID- ORCID: 0000-0002-9283-0110 AD - Department of Cardiology, Inselspital, University Hospital Bern, University of Bern, Bern, Switzerland. FAU - Angellotti, Domenico AU - Angellotti D AUID- ORCID: 0000-0002-7941-0779 AD - Department of Cardiology, Inselspital, University Hospital Bern, University of Bern, Bern, Switzerland. FAU - Bartkowiak, Joanna AU - Bartkowiak J AD - Department of Cardiology, Inselspital, University Hospital Bern, University of Bern, Bern, Switzerland. FAU - Kozhuharov, Nikola AU - Kozhuharov N AUID- ORCID: 0000-0003-2086-3690 AD - Department of Cardiology, Inselspital, University Hospital Bern, University of Bern, Bern, Switzerland. FAU - Kovacs, Boldizsar AU - Kovacs B AD - Department of Cardiology, Inselspital, University Hospital Bern, University of Bern, Bern, Switzerland. FAU - Windecker, Stephan AU - Windecker S AUID- ORCID: 0000-0003-2653-6762 AD - Department of Cardiology, Inselspital, University Hospital Bern, University of Bern, Bern, Switzerland. FAU - Reichlin, Tobias AU - Reichlin T AD - Department of Cardiology, Inselspital, University Hospital Bern, University of Bern, Bern, Switzerland. FAU - Praz, Fabien AU - Praz F AUID- ORCID: 0000-0001-5416-165X AD - Department of Cardiology, Inselspital, University Hospital Bern, University of Bern, Bern, Switzerland. FAU - Noti, Fabian AU - Noti F AUID- ORCID: 0000-0002-2719-5149 AD - Department of Cardiology, Inselspital, University Hospital Bern, University of Bern, Bern, Switzerland. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Pacing Clin Electrophysiol JT - Pacing and clinical electrophysiology : PACE JID - 7803944 SB - IM OTO - NOTNLM OT - lead jailing OT - left bundle area pacing OT - transcatheter tricuspid valve implantation EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 23:23 PHST- 2026/08/10 00:00 [revised] PHST- 2026/06/24 00:00 [received] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:23 [entrez] AID - 10.1111/pace.70464 [doi] PST - aheadofprint SO - Pacing Clin Electrophysiol. 2026 Oct 28. doi: 10.1111/pace.70464. PMID- 42951825 OWN - NLM STAT- Publisher LR - 20261028 IS - 1744-5086 (Electronic) IS - 0928-6586 (Linking) DP - 2026 Oct 28 TI - Demographic and Clinical Factors Associated with Fuchs Endothelial Corneal Dystrophy: A Nationwide Population-Based Study. PG - 1-7 LID - 10.1080/09286586.2026.2754406 [doi] AB - PURPOSE: To identify demographic and clinical factors associated with Fuchs endothelial corneal dystrophy (FECD) in Israel. METHODS: We conducted a population-based nested case-control study using data from Israel's largest healthcare provider covering the years 2005 to 2023. FECD patients were identified and compared with ten age and sex-matched controls per case, selected using risk-set sampling. Demographic factors and comorbidities were analyzed using conditional logistic regression. RESULTS: We identified 591 FECD patients. The mean age at diagnosis was 64.35 +/- 11 years; 198 (33.5%) were male. Multivariable analysis revealed several factors significantly associated with FECD: Medium and high SES (OR = 1.308, p = 0.014) and (OR = 1.456, p = 0.017), cataract (OR = 4.836, p < 0.001), skin cancer (OR = 1.362, p = 0.01), keratoconus (OR = 4.349, p = 0.002), hypermetropia (OR = 1.839, p < 0.001), glaucoma (OR = 1.726, p = 0.003) and uveitis (OR = 3.082, p < 0.001). CONCLUSIONS: This population-based study identifies several factors significantly associated with FECD, including medium and high SES, cataract, skin cancer, keratoconus, hypermetropia, glaucoma and uveitis. These findings improve understanding of the demographic and clinical context associated with FECD and inform future longitudinal and mechanistic studies. FAU - Segal, Adi AU - Segal A AD - Ophthalmology, Lady Davis Carmel Medical Center, Haifa, Israel. AD - The Ruth and Bruce Rappaport Faculty of Medicine, Technion - Israel Institute of Technology, Haifa, Israel. FAU - Vaknin, Mordechai AU - Vaknin M AD - Ophthalmology, Lady Davis Carmel Medical Center, Haifa, Israel. FAU - Haddad, Joule AU - Haddad J AD - Department of Community Medicine and Epidemiology, Lady Davis Carmel Medical Center, Haifa, Israel. FAU - Gabison, Eric E AU - Gabison EE AD - Service d'Ophthalmologie, Hospital Fondation Rothschild, Paris, France. FAU - Ben Shaul, Or AU - Ben Shaul O AD - Ophthalmology, Lady Davis Carmel Medical Center, Haifa, Israel. AD - The Ruth and Bruce Rappaport Faculty of Medicine, Technion - Israel Institute of Technology, Haifa, Israel. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Ophthalmic Epidemiol JT - Ophthalmic epidemiology JID - 9435674 SB - IM OTO - NOTNLM OT - Corneal endothelium OT - Epidemiology OT - FECD OT - factors associated with FECD OT - population-based study EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 23:06 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:06 [entrez] AID - 10.1080/09286586.2026.2754406 [doi] PST - aheadofprint SO - Ophthalmic Epidemiol. 2026 Oct 28:1-7. doi: 10.1080/09286586.2026.2754406. PMID- 42951807 OWN - NLM STAT- Publisher LR - 20261028 IS - 2095-9281 (Electronic) IS - 2095-9273 (Linking) DP - 2026 Oct 15 TI - 3d orbital occupancy regulation stabilizes *CO(2)(delta)(-) intermediate for efficient urea electrosynthesis. LID - S2095-9273(26)01258-2 [pii] LID - 10.1016/j.scib.2026.10.028 [doi] AB - Electrosynthesis of urea via C-N coupling of CO(2) with nitrate (NO(3)(-)) has emerged as a fascinating energy-efficient technology. However, the inherent disparity in activation barriers between CO(2) and NO(3)(-) severely impedes efficient C-N bond formation. Herein, we present an unconventional CO(2)-mediated C-N coupling pathway enabled by d-orbital modulation of Cu single atoms anchored on titanium carbide (Cu(SACs)/TiC). Combined theoretical calculations and in-situ spectroscopic analyses reveal that strong electronic metal-support interaction (EMSI) downshifts the d band center of Cu(SACs), while spin-charge injection increases Cu [Formula: see text] occupancy from t(2g)(6)e(g)(3) to t(2g)(6)e(g)(4). This electronic reconfiguration of Cu(SACs) d-orbital enables controlled electron transfer into the CO(2) pi* orbitals, facilitating the electron-transfer (ET) to generate *CO(2)(delta)(-) intermediate. Simultaneously, the bonding orbitals of *NO(2) generated on the TiC directly couple with the antibonding orbital of *CO(2)(delta)(-), promoting C-N bond formation toward urea. Consequently, the optimal Cu(SACs)/TiC delivers an outstanding C selectivity of 97.8%, along with an excellent Faradaic efficiency (FE) of 50.1% and yield of 558.8 mug h(-1) mg(cat)(-1) for urea production in the electrolyte of 0.05 mol L(-1) KNO(3) and 0.1 mol L(-1) KHCO(3). This work highlights a unique C-N coupling pathway via d-orbital engineering for efficient electrochemical C-N bond formation from CO(2) and nitrogenous feedstocks. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Luo, Yu AU - Luo Y AD - Key Laboratory of Jiangxi Province for Persistent Pollutants Prevention Control and Resource Reuse, National-Local Joint Engineering Research Center of Heavy Metals Pollutants Control and Resource Utilization, Nanchang Hangkong University, Nanchang 330063, China; School of Materials Science and Engineering, Beihang University, Beijing 100191, China. FAU - Lu, Chen-Qi AU - Lu CQ AD - Key Laboratory of Jiangxi Province for Persistent Pollutants Prevention Control and Resource Reuse, National-Local Joint Engineering Research Center of Heavy Metals Pollutants Control and Resource Utilization, Nanchang Hangkong University, Nanchang 330063, China. FAU - Xiao, Chuang AU - Xiao C AD - Key Laboratory of Jiangxi Province for Persistent Pollutants Prevention Control and Resource Reuse, National-Local Joint Engineering Research Center of Heavy Metals Pollutants Control and Resource Utilization, Nanchang Hangkong University, Nanchang 330063, China. FAU - Ding, Lin AU - Ding L AD - Key Laboratory of Jiangxi Province for Persistent Pollutants Prevention Control and Resource Reuse, National-Local Joint Engineering Research Center of Heavy Metals Pollutants Control and Resource Utilization, Nanchang Hangkong University, Nanchang 330063, China. FAU - Chu, Chu AU - Chu C AD - Key Laboratory of Jiangxi Province for Persistent Pollutants Prevention Control and Resource Reuse, National-Local Joint Engineering Research Center of Heavy Metals Pollutants Control and Resource Utilization, Nanchang Hangkong University, Nanchang 330063, China; School of Materials Science and Engineering, Beihang University, Beijing 100191, China. FAU - Liu, Ling-Ling AU - Liu LL AD - Key Laboratory of Jiangxi Province for Persistent Pollutants Prevention Control and Resource Reuse, National-Local Joint Engineering Research Center of Heavy Metals Pollutants Control and Resource Utilization, Nanchang Hangkong University, Nanchang 330063, China. FAU - Zhang, Long-Shuai AU - Zhang LS AD - Key Laboratory of Jiangxi Province for Persistent Pollutants Prevention Control and Resource Reuse, National-Local Joint Engineering Research Center of Heavy Metals Pollutants Control and Resource Utilization, Nanchang Hangkong University, Nanchang 330063, China. FAU - Tian, Lei AU - Tian L AD - Key Laboratory of Jiangxi Province for Persistent Pollutants Prevention Control and Resource Reuse, National-Local Joint Engineering Research Center of Heavy Metals Pollutants Control and Resource Utilization, Nanchang Hangkong University, Nanchang 330063, China. FAU - Liu, Zhao-Qing AU - Liu ZQ AD - School of Chemistry and Chemical Engineering, Institute of Clean Energy and Materials, Key Laboratory for Clean Energy and Materials, Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou 510006, China; School of Chemistry, South China Normal University, Guangzhou 510006, China. Electronic address: lzqgzhu@gzhu.edu.cn. FAU - Zou, Jian-Ping AU - Zou JP AD - Key Laboratory of Jiangxi Province for Persistent Pollutants Prevention Control and Resource Reuse, National-Local Joint Engineering Research Center of Heavy Metals Pollutants Control and Resource Utilization, Nanchang Hangkong University, Nanchang 330063, China. Electronic address: zjp_112@126.com. LA - eng PT - Journal Article DEP - 20261015 PL - Netherlands TA - Sci Bull (Beijing) JT - Science bulletin JID - 101655530 SB - IM OTO - NOTNLM OT - Cu single atom OT - C-N coupling OT - Electronic metal-support interaction OT - Urea electrosynthesis OT - d-orbital modulation EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 23:04 PHST- 2026/07/08 00:00 [received] PHST- 2026/08/23 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:04 [entrez] AID - S2095-9273(26)01258-2 [pii] AID - 10.1016/j.scib.2026.10.028 [doi] PST - aheadofprint SO - Sci Bull (Beijing). 2026 Oct 15:S2095-9273(26)01258-2. doi: 10.1016/j.scib.2026.10.028. PMID- 42951786 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-9809 (Electronic) IS - 1556-8598 (Linking) DP - 2026 Oct 28 TI - Valuable Role of Fluorodeoxyglucose-PET in Therapeutic Decision Making and Theranostics of Neurologic Disorders in Conjunction with Non-Fluorodeoxyglucose Tracers. LID - S1556-8598(26)00130-6 [pii] LID - 10.1016/j.cpet.2026.09.021 [doi] AB - Brain (1)(8)F-fluorodeoxyglucose (FDG) positron emission tomography (PET) is an important functional imaging modality for evaluating glucose metabolism. Over time, it has been observed that various metabolic patterns can support diagnoses, inform prognosis, guide therapeutic decision-making, and assess treatment response. Clinical applications span seizures, brain tumors, traumatic brain injury, neuroinflammatory and infectious disorders, neurodegenerative diseases, and other chronic neurological conditions. Although FDG is not itself a theranostic radiopharmaceutical, its ability to characterize metabolism may contribute to treatment planning, particularly in molecularly targeted neuro-oncology. This chapter reviews established and investigational applications of brain FDG-PET, emphasizing disease characterization, therapeutic selection, and response assessment. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Newberg, Andrew B AU - Newberg AB AD - Department of Integrative Medicine and Nutritional Sciences, Research at the Marcus Institute of Integrative Health, Philadelphia, PA, USA; Department of Radiology, Research at the Marcus Institute of Integrative Health, Philadelphia, PA, USA; Department of Radiology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USA. Electronic address: Andrew.newberg@jefferson.edu. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - PET Clin JT - PET clinics JID - 101260152 SB - IM OTO - NOTNLM OT - 18F fluorodeoxyglucose (FDG) OT - Brain imaging OT - Cerebral glucose metabolism OT - Neurologic disorders OT - Positron emission tomography (PET) OT - Theranostics OT - Therapy COIS- Disclosures The author have nothing to disclose. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 23:04 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:04 [entrez] AID - S1556-8598(26)00130-6 [pii] AID - 10.1016/j.cpet.2026.09.021 [doi] PST - aheadofprint SO - PET Clin. 2026 Oct 28:S1556-8598(26)00130-6. doi: 10.1016/j.cpet.2026.09.021. PMID- 42951781 OWN - NLM STAT- Publisher LR - 20261028 IS - 1097-6841 (Electronic) IS - 0022-3913 (Linking) DP - 2026 Oct 28 TI - Biomechanical evaluation of shield design in multiple anterior implant restorations: A three-dimensional finite element analysis. LID - S0022-3913(26)00675-X [pii] LID - 10.1016/j.prosdent.2026.10.003 [doi] AB - STATEMENT OF PROBLEM: The socket shield technique (SST) has demonstrated benefits in preserving esthetic outcomes. However, the biomechanical behavior associated with its application in multiple adjacent implant restorations remains unclear. PURPOSE: The purpose of this finite element analysis study was to evaluate the biomechanical influence of classic and Half-C shield designs in bilateral maxillary central incisor and combined central-lateral incisor implant restorations and to provide biomechanical evidence to support shield selection in multitooth SST applications. MATERIAL AND METHODS: A 3-dimensional model of the complete maxilla was reconstructed. Two common anterior implant restoration scenarios were simulated: bilateral central incisors and combined central-lateral incisors. Classic and Half-C shields were generated, and 8 finite element models were constructed. Time-dependent loading was applied to the crowns. Stress distributions in the implant region and strain distributions in the surrounding bone were compared. Statistical analysis was performed using a general linear model univariate procedure (alpha=.05). RESULTS: The Half-C shield exhibited lower overall stress and produced a more balanced strain distribution than the classic shield. Shield type and socket aspects both significantly influenced the strain in the surrounding bone (P<.001). The Half-C configuration reduced mesiodistal strain and showed synergistic stress-relieving effects when applied on both teeth. CONCLUSIONS: The Half-C shield provided biomechanical advantages by reducing peak stress and moderating strain distribution, indicating better mechanical performance than the classic shield and supporting its use in 2-tooth restorations. CI - Copyright (c) 2026 Editorial Council for The Journal of Prosthetic Dentistry. Published by Elsevier Inc. All rights reserved. FAU - Zuo, Qianqian AU - Zuo Q AD - Graduate student, Department of Biomedical Engineering, School of Architecture and Environment, Sichuan University, Chengdu, PR China; and Graduate student, Sichuan University Yibin Park, Yibin, PR China. FAU - Teng, Haidong AU - Teng H AD - Graduate student, Department of Mechanics, School of Architecture and Environment, Sichuan University, Chengdu, PR China; and Graduate student, Sichuan University Yibin Park, Yibin, PR China. FAU - Zhang, Yuanli AU - Zhang Y AD - Associate Professor, Department of Medical Technology, Chongqing Three Gorges Medical College, Chongqing, PR China. FAU - Liu, Zhan AU - Liu Z AD - Professor, School of Architecture and Environment, Sichuan University, Chengdu, PR China; Key Lab for Biomechanical Engineering of Sichuan Province, Sichuan University, Chengdu, PR China; and Sichuan University Yibin Park, Yibin Institute of Industrial Technology, Yibin, PR China. Electronic address: bmeliuzhan@163.com. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Prosthet Dent JT - The Journal of prosthetic dentistry JID - 0376364 SB - IM EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 23:04 PHST- 2026/03/10 00:00 [received] PHST- 2026/08/20 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:04 [entrez] AID - S0022-3913(26)00675-X [pii] AID - 10.1016/j.prosdent.2026.10.003 [doi] PST - aheadofprint SO - J Prosthet Dent. 2026 Oct 28:S0022-3913(26)00675-X. doi: 10.1016/j.prosdent.2026.10.003. PMID- 42951775 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-0097 (Electronic) IS - 0109-5641 (Linking) DP - 2026 Oct 28 TI - Effect of epigallocatechin-3-gallate on resin-dentin bond strength: A systematic review and meta-analysis of dentin pretreatment and adhesive incorporation strategies. LID - S0109-5641(26)00468-9 [pii] LID - 10.1016/j.dental.2026.10.011 [doi] AB - OBJECTIVES: To systematically evaluate the effect of epigallocatechin-3-gallate (EGCG) on dentin bond strength and bonding durability according to its application strategy. METHODS: This systematic review was conducted in accordance with the PRISMA 2020 statement and registered in PROSPERO (CRD420251003015). PubMed/MEDLINE, Web of Science, EMBASE, Scopus, and the Cochrane Library were searched from inception to July 2026. Clinical, in situ, and in vitro studies evaluating EGCG as a dentin pretreatment or incorporated into dental adhesives were eligible. Study selection, data extraction, and risk-of-bias assessment were performed independently by two reviewers using the RoBDEMAT and RoB 2 tools. Meta-analyses were performed using RevMan to evaluate the effects of EGCG on microtensile bond strenght, considering immediate and long-term outcomes, using random-effects models and reporting mean differences (MD) with 95% confidence intervals (CI). RESULTS: 35 studies met the eligibility criteria. 17 evaluated EGCG as a dentin pretreatment, 15 investigated its incorporation into dental adhesives, and three assessed both strategies. Meta-analysis demonstrated that dentin pretreatment with EGCG significantly improved immediate and long-term microtensile bond strength. When incorporated into adhesives, EGCG improved bond strength after aging only in etch-and-rinse adhesive systems, whereas no significant effects were observed for self-etch adhesives. Risk-of-bias assessment indicated generally favorable methodological quality, although some concerns were identified in both clinical and in vitro studies. SIGNIFICANCE: EGCG may improve resin-dentin bond strength, although its effectiveness depends on the application strategy and adhesive system. Dentin pretreatment showed more favorable results than incorporation into adhesive formulations. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Nunes, Gabriel Pereira AU - Nunes GP AD - Department of Prosthodontics and Periodontics, Piracicaba Dental School, Sao Paulo State University of Campinas (UNICAMP), Piracicaba, SP, Brazil. FAU - de Oliveira Alves, Renata AU - de Oliveira Alves R AD - Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, Sao Paulo University (USP), Bauru, SP, Brazil. FAU - Peres, Georgia Rondo AU - Peres GR AD - Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, Sao Paulo University (USP), Bauru, SP, Brazil. FAU - Alves de Toledo, Priscila Toninatto AU - Alves de Toledo PT AD - Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, Sao Paulo University (USP), Bauru, SP, Brazil. FAU - Chrisostomo, Daniella Alvim AU - Chrisostomo DA AD - Dental Research Institute, Faculty of Dentistry, University of Toronto, 124 Edward Street, Toronto, ON, M5G 1G6, Canada. FAU - E Oliveira, Hiskell Francine Fernandes AU - E Oliveira HFF AD - Department of Prosthodontics and Periodontics, Piracicaba Dental School, Sao Paulo State University of Campinas (UNICAMP), Piracicaba, SP, Brazil. FAU - Prakki, Anuradha AU - Prakki A AD - Dental Research Institute, Faculty of Dentistry, University of Toronto, 124 Edward Street, Toronto, ON, M5G 1G6, Canada. Electronic address: a.prakki@dentistry.utoronto.ca. FAU - Duque, Cristiane AU - Duque C AD - Faculty of Dental Medicine, Centre for Interdisciplinary Research in Health (CIIS), Universidade Catolica Portuguesa, Viseu, Portugal. Electronic address: cduque@ucp.pt. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - Dent Mater JT - Dental materials : official publication of the Academy of Dental Materials JID - 8508040 SB - IM OTO - NOTNLM OT - Adhesion OT - Dentin OT - Dentin-Bonding Agents OT - Epigallocatechin-3-gallate COIS- Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: n/a If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 23:04 PHST- 2026/04/07 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:04 [entrez] AID - S0109-5641(26)00468-9 [pii] AID - 10.1016/j.dental.2026.10.011 [doi] PST - aheadofprint SO - Dent Mater. 2026 Oct 28:S0109-5641(26)00468-9. doi: 10.1016/j.dental.2026.10.011. PMID- 42951762 OWN - NLM STAT- Publisher LR - 20261028 IS - 2405-4569 (Electronic) IS - 2405-4569 (Linking) DP - 2026 Oct 28 TI - The Prognostic Value of Lymphovascular Invasion in Combination with Nodal Stage on Disease-specific Survival in Patients with Bladder Cancer Following Upfront Radical Cystectomy. LID - S2405-4569(26)00200-2 [pii] LID - 10.1016/j.euf.2026.09.004 [doi] AB - The prognostic value of lymphovascular invasion (LVI) after radical cystectomy (RC) remains debated, particularly in relation to pathological tumor stage (pT) and pathological nodal status (pN). We analyzed the strongest prognostic factors for disease-specific survival (DSS) in a multicenter study with local pathology review, including 1229 patients with cT1-4aN0M0 urothelial bladder cancer (BC) treated with upfront RC (1986-2020). A classification and regression tree (CART) analysis was used to prioritize prognostic variables. CART identified pN status as the primary discriminator, followed by LVI as the second strongest prognostic factor within pN-negative disease. LVI was present in 584 patients (48%). Median follow-up was 4.1 yr. In multivariable Cox regression models, LVI was independently associated with DSS in both the pN-negative (hazard ratio [HR] 1.50, 95% confidence interval (CI) = 1.16-1.94; p = 0.002) and pN-positive (HR 1.38, 95%CI = 1.04-1.83; p = 0.03) cohorts. CART defined three prognostic groups: favorable prognosis (pN negative/LVI negative), intermediate prognosis (pN negative/LVI positive), and poor prognosis (pN positive), with 5-yr DSS of 65%, 48%, and 25%, respectively. Among patients with poor prognosis, adjuvant chemotherapy (AC) was associated with longer DSS (HR 0.47; p < 0.001). In the intermediate-prognosis group, receipt of AC was not statistically associated with DSS (HR 0.66, 95%CI 0.39-1.10; p = 0.10). Combined assessment of pN status and LVI provides clinically meaningful postoperative risk stratification that may help refine postoperative risk assessment, surveillance planning, and multidisciplinary discussion regarding adjuvant treatment. CI - Copyright (c) 2026 The Author(s). Published by Elsevier B.V. All rights reserved. FAU - van Gennep, Erik J AU - van Gennep EJ AD - Department of Surgical Oncology (Urology), Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, The Netherlands; Department of Urology, Leiden University Medical Center, Leiden, The Netherlands. Electronic address: e.j.van_gennep@lumc.nl. FAU - Claps, Francesco AU - Claps F AD - Department of Surgical Oncology (Urology), Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, The Netherlands; Department of Urology, Leiden University Medical Center, Leiden, The Netherlands; Urological Clinic, Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Italy; Oncological Urology, Veneto Institute of Oncology IOV - IRCCS, Padua, Italy. FAU - KleinJan, Gijs H AU - KleinJan GH AD - Department of Urology, Leiden University Medical Center, Leiden, The Netherlands. FAU - Bostrom, Peter J AU - Bostrom PJ AD - Department of Surgery (Urology), University Health Network, Princess Margaret Cancer Center, University of Toronto, Toronto, Canada; Department of Urology, Turku University Hospital and University of Turku, Turku, Finland. FAU - Shariat, Shahrokh F AU - Shariat SF AD - Department of Urology, University of Texas Southwestern Medical Center, Dallas, USA; Department of Urology, Comprehensive Cancer Center, Medical University of Vienna, Vienna, Austria; Department of Urology, Weill Cornell Medical College, New York, USA; Department of Urology, Second Faculty of Medicine, Charles University, Prague, Czech Republic; Institute for Urology and Reproductive Health, I.M. Sechenov First Moscow State Medical University, Moscow, Russia. FAU - Neuzillet, Yann AU - Neuzillet Y AD - Department of Surgical Oncology (Urology), Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, The Netherlands; Institut Curie, CNRS, UMR144, Molecular Oncology Team, PSL Research University, Paris, France; Department of Urology, Hopital Foch, UVSQ-Paris-Saclay University, Suresnes, France. FAU - Zlotta, Alexandre R AU - Zlotta AR AD - Department of Surgery (Urology), University Health Network, Princess Margaret Cancer Center, University of Toronto, Toronto, Canada. FAU - Amodeo, Antonio AU - Amodeo A AD - Oncological Urology, Veneto Institute of Oncology IOV - IRCCS, Padua, Italy. FAU - Eckstein, Markus AU - Eckstein M AD - Institute of Pathology, University Hospital Erlangen, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Erlangen, Germany. FAU - Mertens, Laura S AU - Mertens LS AD - Department of Surgical Oncology (Urology), Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, The Netherlands. FAU - Bussani, Rossana AU - Bussani R AD - Department of Pathology, University of Trieste, Trieste, Italy. FAU - Burger, Maximilian AU - Burger M AD - Department of Urology, Caritas St Josef Medical Center, University of Regensburg, Regensburg, Germany. FAU - Boormans, Joost L AU - Boormans JL AD - Department of Urology, Erasmus MC Cancer Institute, University Medical Center, Rotterdam, The Netherlands. FAU - Wullich, Bernd AU - Wullich B AD - Department of Urology and Pediatric Urology, University Hospital Erlangen, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Erlangen, Germany. FAU - Contieri, Roberto AU - Contieri R AD - Department of Surgical Oncology (Urology), Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, The Netherlands. FAU - Hartmann, Arndt AU - Hartmann A AD - Institute of Pathology, University Hospital Erlangen, Friedrich-Alexander-Universitat Erlangen-Nurnberg, Erlangen, Germany. FAU - Mayr, Roman AU - Mayr R AD - Department of Urology, Caritas St Josef Medical Center, University of Regensburg, Regensburg, Germany. FAU - Trombetta, Carlo AU - Trombetta C AD - Urological Clinic, Department of Medicine, Surgery and Health Sciences, University of Trieste, Trieste, Italy. FAU - Pavan, Nicola AU - Pavan N AD - Urological Clinic, Department of Precision Medicine in Medical, Surgical and Critical Care, University of Palermo, Palermo, Italy. FAU - Porreca, Angelo AU - Porreca A AD - Urology Department, Humanitas Gavazzeni, Humanitas University, Bergamo, Italy. FAU - Mir, M Carmen AU - Mir MC AD - Department of Urology, Hospital Universitario La Ribera, Valencia, Spain. FAU - Pouessel, Damien AU - Pouessel D AD - Department of Medical Oncology, Claudius Regaud Institute, Toulouse University Cancer Center (IUCT) Oncopole, Toulouse, France. FAU - Roshani, Hossain AU - Roshani H AD - Department of Urology, Haga Teaching Hospital, The Hague, The Netherlands. FAU - van der Kwast, Theo H AU - van der Kwast TH AD - Department of Pathology, University Health Network, Princess Margaret Cancer Center, University of Toronto, Toronto, Canada. FAU - Allory, Yves AU - Allory Y AD - Institut Curie, CNRS, UMR144, Molecular Oncology Team, PSL Research University, Paris, France; Department of Pathology, Institut Curie, Paris, France. FAU - Zuiverloon, Tahlita C M AU - Zuiverloon TCM AD - Department of Urology, Erasmus MC Cancer Institute, University Medical Center, Rotterdam, The Netherlands. FAU - van Leenders, Geert J L H AU - van Leenders GJLH AD - Department of Pathology, Erasmus MC Cancer Institute, University Medical Center, Rotterdam, The Netherlands. FAU - Lotan, Yair AU - Lotan Y AD - Department of Urology, University of Texas Southwestern Medical Center, Dallas, USA. FAU - van Rhijn, Bas W G AU - van Rhijn BWG AD - Department of Surgical Oncology (Urology), Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Amsterdam, The Netherlands; Department of Urology, Leiden University Medical Center, Leiden, The Netherlands; Department of Surgery (Urology), University Health Network, Princess Margaret Cancer Center, University of Toronto, Toronto, Canada; Department of Urology, Caritas St Josef Medical Center, University of Regensburg, Regensburg, Germany. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Eur Urol Focus JT - European urology focus JID - 101665661 SB - IM OTO - NOTNLM OT - Bladder cancer OT - Lymphovascular invasion OT - Radical cystectomy OT - Survival OT - Urothelial neoplasm EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 23:03 PHST- 2026/04/10 00:00 [received] PHST- 2026/08/18 00:00 [revised] PHST- 2026/09/07 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:03 [entrez] AID - S2405-4569(26)00200-2 [pii] AID - 10.1016/j.euf.2026.09.004 [doi] PST - aheadofprint SO - Eur Urol Focus. 2026 Oct 28:S2405-4569(26)00200-2. doi: 10.1016/j.euf.2026.09.004. PMID- 42951752 OWN - NLM STAT- Publisher LR - 20261028 IS - 1499-3872 (Print) DP - 2026 Oct 11 TI - Ex vivo liver resection and autotransplantation: Comparative risk of complications in left versus right liver grafts. LID - S1499-3872(26)00160-8 [pii] LID - 10.1016/j.hbpd.2026.09.007 [doi] AB - BACKGROUND: For conventionally unresectable hepatic alveolar echinococcosis (HAE), ex vivo liver resection and autotransplantation (ELRA) enables R0 resection. Left and right lobe grafts present distinct complication risks that guide surgical planning and postoperative management. However, evidence comparing these graft outcomes in HAE remains limited. METHODS: We analyzed 142 ELRA patients with HAE (2010-2023), grouped as left lobe (n = 98) or right lobe (n = 44). Propensity score matching (PSM) paired 42 patients per group. Least absolute shrinkage and selection operator (LASSO) regression identified predictors of biliary and outflow complications, followed by multivariate logistic regression to build a prognostic model. Model performance was evaluated using receiver operating characteristic (ROC) curves, calibration plots, and decision curve analysis. RESULTS: No intraoperative deaths occurred. Pleural effusion was the most common complication (67%). Right lobe transplantation was associated with longer surgical time and higher blood loss (P < 0.05). Biliary complications were more frequent in the right lobe group (38% vs. 19%, P < 0.05), with independent predictors including hepatic bile duct dilation [odds ratio (OR) = 18.82, 95% confidence interval (CI): 5.06-97.35] and right lobe graft type (OR = 6.12, 95% CI: 1.61-31.16). Outflow complications were more common in the left lobe group (38% vs. 17%; P < 0.05), with independent predictors including artificial blood vessel (OR = 3.42, 95% CI: 1.09-10.80), right lobe graft type (OR = 0.29, 95% CI: 0.09-0.96), and portal hypertension (OR = 3.93, 95% CI: 1.01-15.30). Predictive models showed strong discrimination for biliary [area under the receiver operating characteristic curve (AUC) = 0.83; 95% CI: 0.74-0.91] and outflow complications (AUC = 0.80, 95% CI: 0.68-0.93), with decision curve analysis confirming clinical utility. CONCLUSIONS: A high-accuracy prognostic model was developed for predicting ELRA complications, aiding individualized surgical decision-making. CI - Copyright (c) 2026 First Affiliated Hospital, Zhejiang University School of Medicine in China. Published by Elsevier B.V. All rights reserved. FAU - Yilihaer, Yierfan AU - Yilihaer Y AD - Department of Hepatobiliary and Echinococcosis Surgery, Digestive and Vascular Surgery Center, the First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830054, China; State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi, 830054, China. FAU - Tulahong, Alimu AU - Tulahong A AD - Department of Hepatobiliary and Echinococcosis Surgery, Digestive and Vascular Surgery Center, the First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830054, China; State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi, 830054, China. FAU - Wen, Hao AU - Wen H AD - State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi, 830054, China. FAU - Jiang, Tie-Min AU - Jiang TM AD - Department of Hepatobiliary and Echinococcosis Surgery, Digestive and Vascular Surgery Center, the First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830054, China; State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi, 830054, China. FAU - Shao, Ying-Mei AU - Shao YM AD - Department of Hepatobiliary and Echinococcosis Surgery, Digestive and Vascular Surgery Center, the First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830054, China; State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi, 830054, China. FAU - Aji, Tuerganaili AU - Aji T AD - Department of Hepatobiliary and Echinococcosis Surgery, Digestive and Vascular Surgery Center, the First Affiliated Hospital of Xinjiang Medical University, Urumqi, 830054, China; State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, Xinjiang Medical University, Urumqi, 830054, China. Electronic address: tuergan78@sina.com. LA - eng PT - Journal Article DEP - 20261011 PL - Singapore TA - Hepatobiliary Pancreat Dis Int JT - Hepatobiliary & pancreatic diseases international : HBPD INT JID - 101151457 SB - IM OTO - NOTNLM OT - Biliary complications OT - Ex vivo liver resection OT - Hepatectomy OT - Hepatic alveolar echinococcosis OT - Liver transplantation OT - Outflow complications COIS- Competing interest No benefits in any form have been received or will be received from a commercial party related directly or indirectly to the subject of this article. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 23:03 PHST- 2025/07/03 00:00 [received] PHST- 2025/09/23 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:03 [entrez] AID - S1499-3872(26)00160-8 [pii] AID - 10.1016/j.hbpd.2026.09.007 [doi] PST - aheadofprint SO - Hepatobiliary Pancreat Dis Int. 2026 Oct 11:S1499-3872(26)00160-8. doi: 10.1016/j.hbpd.2026.09.007. PMID- 42951750 OWN - NLM STAT- Publisher LR - 20261028 IS - 1532-8422 (Electronic) IS - 1053-0770 (Linking) DP - 2026 Sep 26 TI - Peripheral Nerve Blocks for Postoperative Pain Management in Cardiothoracic Surgery: A Systematic Review and Meta-analysis. LID - S1053-0770(26)00963-8 [pii] LID - 10.1053/j.jvca.2026.09.047 [doi] AB - OBJECTIVE: To evaluate the effectiveness, comparative effectiveness, and safety of peripheral nerve blocks for postoperative pain management after cardiothoracic surgery. DESIGN: Systematic review and meta-analysis of randomized controlled trials, with strength of evidence rated using the Agency for Healthcare Research and Quality methodology (PROSPERO CRD42024549561). SETTING: Perioperative care settings in studies published between 2013 and 2024. PARTICIPANTS: Adults undergoing open or minimally invasive cardiothoracic surgery. INTERVENTIONS: Peripheral nerve blocks, including chest wall blocks and erector spinae plane blocks, compared with sham, no block, local infiltration, or neuraxial techniques. MEASUREMENTS AND MAIN RESULTS: A total of 104 randomized controlled trials were included. Chest wall blocks reduced 24-hour pain scores and opioid consumption compared with sham or no block in both open cardiac and minimally invasive thoracic surgery. Erector spinae plane blocks demonstrated similar effects, with no clinically meaningful differences compared with chest wall blocks. Peripheral nerve blocks provided analgesia comparable to neuraxial techniques across several outcomes. Reported adverse events were rare and inconsistently defined. CONCLUSIONS: Peripheral nerve blocks provide some reductions in postoperative pain and opioid use after cardiothoracic surgery and appear to have a favorable safety profile. Chest wall blocks and erector spinae plane blocks were found not to differ in effectiveness, and both offer analgesia comparable to neuraxial techniques. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Southwell, Bronwyn J AU - Southwell BJ AD - Department of Anesthesiology, University of Minnesota, Minneapolis, MN; Center for Learning Health System Sciences, University of Minnesota, Minneapolis, MN. Electronic address: South095@umn.edu. FAU - Parikh, Romil R AU - Parikh RR AD - School of Public Health, University of Minnesota, Minneapolis, MN. FAU - Brandt, Sallee AU - Brandt S AD - Center for Learning Health System Sciences, University of Minnesota, Minneapolis, MN; School of Public Health, University of Minnesota, Minneapolis, MN. FAU - Choconta-Piraquive, Luz Angela AU - Choconta-Piraquive LA AD - School of Public Health, University of Minnesota, Minneapolis, MN. FAU - Forte, Mary L AU - Forte ML AD - School of Public Health, University of Minnesota, Minneapolis, MN. FAU - Pagadala, Swathi AU - Pagadala S AD - School of Public Health, University of Minnesota, Minneapolis, MN. FAU - Persson, Drew J AU - Persson DJ AD - School of Public Health, University of Minnesota, Minneapolis, MN; University of Minnesota Medical School, Minneapolis, MN. FAU - Kolbow, Madison M AU - Kolbow MM AD - University of Minnesota Medical School, Minneapolis, MN. FAU - Lott, Gabriella L AU - Lott GL AD - University of Minnesota Medical School, Minneapolis, MN. FAU - Claussen, Amy M AU - Claussen AM AD - School of Public Health, University of Minnesota, Minneapolis, MN. FAU - Yam, Hawking AU - Yam H AD - School of Public Health, University of Minnesota, Minneapolis, MN. FAU - Duval, Sue AU - Duval S AD - Department of Medicine, University of Minnesota, Minneapolis, MN. FAU - Belani, Kumar AU - Belani K AD - Department of Anesthesiology, University of Minnesota, Minneapolis, MN. FAU - Tignanelli, Christopher J AU - Tignanelli CJ AD - Center for Learning Health System Sciences, University of Minnesota, Minneapolis, MN; Department of Surgery, University of Minnesota, Minneapolis, MN. FAU - Banik, Ratan AU - Banik R AD - Department of Anesthesiology, University of Minnesota, Minneapolis, MN. FAU - Butler, Mary E AU - Butler ME AD - Center for Learning Health System Sciences, University of Minnesota, Minneapolis, MN; School of Public Health, University of Minnesota, Minneapolis, MN. LA - eng PT - Journal Article DEP - 20260926 PL - United States TA - J Cardiothorac Vasc Anesth JT - Journal of cardiothoracic and vascular anesthesia JID - 9110208 SB - IM OTO - NOTNLM OT - cardiothoracic surgery OT - multimodal pain management OT - systematic review COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this manuscript. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 23:03 PHST- 2026/04/10 00:00 [received] PHST- 2026/08/02 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:03 [entrez] AID - S1053-0770(26)00963-8 [pii] AID - 10.1053/j.jvca.2026.09.047 [doi] PST - aheadofprint SO - J Cardiothorac Vasc Anesth. 2026 Sep 26:S1053-0770(26)00963-8. doi: 10.1053/j.jvca.2026.09.047. PMID- 42951747 OWN - NLM STAT- Publisher LR - 20261028 IS - 1558-4240 (Electronic) IS - 0749-0720 (Linking) DP - 2026 Sep 22 TI - Considerations for the Management of Ectoparasites on Cattle and Other Ruminants with Pesticides. LID - S0749-0720(26)00063-0 [pii] LID - 10.1016/j.cvfa.2026.09.004 [doi] AB - Pesticides are critically important tools available for the management of ectoparasites in cattle and other ruminants. This review covers important information to consider before selecting and deploying pesticidal products for management of ectoparasites, including pesticide classes and their modes of action, product types, and complications to management plans due to pesticide resistance in the ectoparasites. The goal of this review is to provide a useful reference for veterinarians, animal scientists, producers, and researchers as they incorporate pesticides into their ectoparasite management programs. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Burgess, Edwin R 4th AU - Burgess ER 4th AD - Department of Entomology and Nematology, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA. Electronic address: edwinburgess@ufl.edu. FAU - Scott, Jeffrey G AU - Scott JG AD - Department of Entomology, Cornell University, Ithaca, NY 14853, USA. LA - eng PT - Journal Article PT - Review DEP - 20260922 PL - United States TA - Vet Clin North Am Food Anim Pract JT - The Veterinary clinics of North America. Food animal practice JID - 8511905 SB - IM OTO - NOTNLM OT - Cattle OT - Ectoparasite OT - Insecticide OT - Pesticide OT - Ruminant OT - Veterinary science COIS- Disclosure The authors have nothing to disclose. EDAT- 2026/10/29 05:30 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 23:03 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/29 05:30 [pubmed] PHST- 2026/10/28 23:03 [entrez] AID - S0749-0720(26)00063-0 [pii] AID - 10.1016/j.cvfa.2026.09.004 [doi] PST - aheadofprint SO - Vet Clin North Am Food Anim Pract. 2026 Sep 22:S0749-0720(26)00063-0. doi: 10.1016/j.cvfa.2026.09.004. PMID- 42951730 OWN - NLM STAT- Publisher LR - 20261028 IS - 1468-2060 (Electronic) IS - 0003-4967 (Linking) DP - 2026 Oct 28 TI - Real‑world assessment of the effectiveness and response profiles of biologic DMARDs in psoriatic arthritis: findings from an emulated multicentre clinical trial. LID - S0003-4967(26)00559-5 [pii] LID - 10.1016/j.ard.2026.09.008 [doi] AB - OBJECTIVES: To evaluate 6-month effectiveness and long-term drug-retention rates of biologic disease-modifying antirheumatic drugs (bDMARDs) in psoriatic arthritis (PsA) using real-world data analysed within an emulated multicentre clinical trial framework. Secondary objectives included the characterisation of response profiles and the identification of possible predictors of clinical response across bDMARD classes. METHODS: Between January 2022 and June 2025, consecutive patients with moderate-to-high PsA disease activity initiating a bDMARD were prospectively enrolled in a multicentre Gruppo Italiano di Ricerca in Reumatologia Clinica e Sperimentale study. Real-world data were analysed within a target trial emulation framework comparing tumour necrosis factor (TNF) inhibitors, interleukin (IL)-17 inhibitors, and IL-23-pathway inhibitors. Propensity score weighting was used to improve comparability in measured baseline characteristics across treatment groups. Effectiveness was assessed through longitudinal changes in the Disease Activity Index for Psoriatic Arthritis (DAPSA) over 6 months. Subsequent long-term drug retention was evaluated using a 6-month landmark analysis restricted to patients who remained on the index bDMARD at month 6. Potential clinical factors associated with treatment response and discontinuation were also explored. RESULTS: Overall, 990 patients were included: 342 initiated TNF inhibitors, 313 IL-17 inhibitors, and 335 IL-23-pathway inhibitors. DAPSA decreased significantly over 6 months in all 3 treatment groups, with no statistically significant between-class differences in adjusted longitudinal trajectories. Among patients who remained on the index bDMARD through month 6, the adjusted analyses did not identify statistically significant between-class differences in subsequent long-term drug retention. Shorter disease duration was associated with more favourable outcomes in selected exploratory analyses, whereas associations with previous bDMARD exposure were inconsistent. Additional class-specific associations were identified but should be considered exploratory and hypothesis-generating. Adverse-event-related discontinuations documented in routine clinical records were described; however, the study was not designed to support formal comparative safety conclusions. CONCLUSIONS: In this real-world emulated multicentre clinical trial, bDMARDs showed comparable overall trajectories of short-term effectiveness and long-term treatment persistence in PsA. The identification of partially distinct response patterns may support a more individualised approach to bDMARD selection. CI - Copyright (c) 2026 The Author(s). Published by Elsevier B.V. All rights reserved. FAU - Ruscitti, Piero AU - Ruscitti P AD - Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy. Electronic address: piero.ruscitti@univaq.it. FAU - Masedu, Francesco AU - Masedu F AD - Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy. FAU - Pantano, Ilenia AU - Pantano I AD - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Naples, Italy. FAU - Annunziata, Marco AU - Annunziata M AD - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Naples, Italy. FAU - Berardicurti, Onorina AU - Berardicurti O AD - Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy; Research and Clinical Unit of Immunorheumatology, Department of Medicine and Surgery, Universita Campus Bio-Medico di Roma, Rome, Italy. FAU - Celletti, Eleonora AU - Celletti E AD - Rheumatology Unit, Clinica Medica Institute, Ospedale "SS. Annunziata" di Chieti/Universita "G. d'Annunzio", Chieti, Italy. FAU - Ciaffi, Jacopo AU - Ciaffi J AD - Medicine & Rheumatology Unit, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy; Department of Biomedical and Neuromotor Sciences (DIBINEM), Alma Mater Studiorum University of Bologna, Bologna, Italy. FAU - Cipriani, Chiara AU - Cipriani C AD - Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy. FAU - Corrado, Addolorata AU - Corrado A AD - Rheumatology Unit, Department of Medical and Surgical Sciences, Azienda Ospedaliero-Universitaria Policlinico Riuniti di Foggia, Universita degli Studi di Foggia, Foggia, Italy. FAU - Costa, Luisa AU - Costa L AD - Rheumatology Unit, Department of Clinical Medicine and Surgery, University of Naples Federico II, Naples, Italy. FAU - Dionisi, Alice AU - Dionisi A AD - Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy. FAU - Di Penta, Myriam AU - Di Penta M AD - Rheumatology Unit, Clinica Medica Institute, Ospedale "SS. Annunziata" di Chieti/Universita "G. d'Annunzio", Chieti, Italy. FAU - Gandolfo, Saviana AU - Gandolfo S AD - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Naples, Italy; Rheumatology Unit, Ospedale del Mare, Naples, Italy. FAU - La Barbera, Lidia AU - La Barbera L AD - Rheumatology Section, Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties, University Hospital P Giaccone, University of Palermo, Palermo, Italy. FAU - Lo Gullo, Alberto AU - Lo Gullo A AD - Rheumatology Unit, Papardo Hospital, Messina, Italy. FAU - Mariani, Francesco Maria AU - Mariani FM AD - Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy. FAU - Mauro, Daniele AU - Mauro D AD - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Naples, Italy. FAU - Mellone, Martina AU - Mellone M AD - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Naples, Italy. FAU - Navarini, Luca AU - Navarini L AD - Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy; Research and Clinical Unit of Immunorheumatology, Department of Medicine and Surgery, Universita Campus Bio-Medico di Roma, Rome, Italy. FAU - Oliva, Marianna AU - Oliva M AD - Rheumatology Unit, Papardo Hospital, Messina, Italy. FAU - Raimondi, Maura AU - Raimondi M AD - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Naples, Italy. FAU - Ricciardi, Riccardo Mattia AU - Ricciardi RM AD - Rheumatology Unit, Clinica Medica Institute, Ospedale "SS. Annunziata" di Chieti/Universita "G. d'Annunzio", Chieti, Italy. FAU - Saracino, Francesca AU - Saracino F AD - Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy; Research and Clinical Unit of Immunorheumatology, Department of Medicine and Surgery, Universita Campus Bio-Medico di Roma, Rome, Italy. FAU - Sgarlata, Giulia AU - Sgarlata G AD - Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy. FAU - Simone, Angelo AU - Simone A AD - Rheumatic and Systemic Autoimmune Diseases Unit, Department of Interdisciplinary Medicine, University of Bari Medical School, Italy. FAU - Shariat Panahi, Azadeh AU - Shariat Panahi A AD - Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy. FAU - Tasso, Marco AU - Tasso M AD - Rheumatology Unit, Department of Clinical Medicine and Surgery, University of Naples Federico II, Naples, Italy. FAU - Tragni, Daniele AU - Tragni D AD - Internal Medicine Unit, Department of Interdisciplinary Medicine, University of Bari Medical School "Aldo Moro", Italy. FAU - Vallocchia, Luca AU - Vallocchia L AD - Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy. FAU - Venafro, Francesca AU - Venafro F AD - Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy. FAU - Viapiano, Federica AU - Viapiano F AD - Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy. FAU - Valenti, Marco AU - Valenti M AD - Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy. FAU - Cantatore, Francesco Paolo AU - Cantatore FP AD - Rheumatology Unit, Department of Medical and Surgical Sciences, Azienda Ospedaliero-Universitaria Policlinico Riuniti di Foggia, Universita degli Studi di Foggia, Foggia, Italy. FAU - Favoino, Elvira AU - Favoino E AD - Rheumatic and Systemic Autoimmune Diseases Unit, Department of Interdisciplinary Medicine, University of Bari Medical School, Italy. FAU - Prete, Marcella AU - Prete M AD - Internal Medicine Unit, Department of Interdisciplinary Medicine, University of Bari Medical School "Aldo Moro", Italy. FAU - Racanelli, Vito AU - Racanelli V AD - Centre for Medical Sciences, University of Trento and Internal Medicine Division, Santa Chiara Hospital, Provincial Health Care Agency (APSS), Trento, Italy. FAU - Perosa, Federico AU - Perosa F AD - Rheumatic and Systemic Autoimmune Diseases Unit, Department of Interdisciplinary Medicine, University of Bari Medical School, Italy. FAU - Ursini, Francesco AU - Ursini F AD - Medicine & Rheumatology Unit, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy; Department of Biomedical and Neuromotor Sciences (DIBINEM), Alma Mater Studiorum University of Bologna, Bologna, Italy. FAU - Caso, Francesco AU - Caso F AD - Rheumatology Unit, Department of Clinical Medicine and Surgery, University of Naples Federico II, Naples, Italy. FAU - Cipriani, Paola AU - Cipriani P AD - Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy. FAU - Caporali, Roberto AU - Caporali R AD - Department of Clinical Science and Community Health, University of Milan, Milan, Italy; Department of Rheumatology and Medical Sciences, ASST Pini-CTO, Milan, Italy. FAU - Scarpa, Raffaele AU - Scarpa R AD - Rheumatology Unit, Department of Clinical Medicine and Surgery, University of Naples Federico II, Naples, Italy. FAU - Giacomelli, Roberto AU - Giacomelli R AD - Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy; Research and Clinical Unit of Immunorheumatology, Department of Medicine and Surgery, Universita Campus Bio-Medico di Roma, Rome, Italy. FAU - Ciccia, Francesco AU - Ciccia F AD - Department of Precision Medicine, University of Campania "Luigi Vanvitelli", Naples, Italy. FAU - Guggino, Giuliana AU - Guggino G AD - Rheumatology Section, Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties, University Hospital P Giaccone, University of Palermo, Palermo, Italy. CN - GIRRCS study group LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Ann Rheum Dis JT - Annals of the rheumatic diseases JID - 0372355 SB - IM COIS- Competing interests All authors declare they have no competing interests. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 23:01 PHST- 2026/05/06 00:00 [received] PHST- 2026/08/26 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 23:01 [entrez] AID - S0003-4967(26)00559-5 [pii] AID - 10.1016/j.ard.2026.09.008 [doi] PST - aheadofprint SO - Ann Rheum Dis. 2026 Oct 28:S0003-4967(26)00559-5. doi: 10.1016/j.ard.2026.09.008. PMID- 42951726 OWN - NLM STAT- Publisher LR - 20261028 IS - 1471-5007 (Electronic) IS - 1471-4922 (Linking) DP - 2026 Oct 28 TI - CLAG3 mutations reduce hemoglobin S-mediated protection against malaria. LID - S1471-4922(26)00311-9 [pii] LID - 10.1016/j.pt.2026.10.003 [doi] AB - Hemoglobin S (HbS), which confers protection against malaria, could exert selective pressure on the genome of Plasmodium falciparum. Forster et al. identified new parasite variants, including two variants shared by CLAG3 paralogs, that reduce the protective effect of HbS. These findings support the hypothesis of coevolution between humans and parasites. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Rihet, Pascal AU - Rihet P AD - Aix-Marseille Univ, INSERM, TAGC, UMR1090, MarMaRa Institute, Marseille, France. Electronic address: pascal.rihet@univ-amu.fr. FAU - Marquet, Sandrine AU - Marquet S AD - Aix-Marseille Univ, INSERM, TAGC, UMR1090, MarMaRa Institute, Marseille, France; CNRS, Marseille, France. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Trends Parasitol JT - Trends in parasitology JID - 100966034 SB - IM OTO - NOTNLM OT - CLAG3 variants OT - FIKK variants OT - Plasmodium falciparum OT - clinical malaria OT - hemoglobin S COIS- Declaration of interests The authors declare no competing interests. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 23:01 PHST- 2026/10/07 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 23:01 [entrez] AID - S1471-4922(26)00311-9 [pii] AID - 10.1016/j.pt.2026.10.003 [doi] PST - aheadofprint SO - Trends Parasitol. 2026 Oct 28:S1471-4922(26)00311-9. doi: 10.1016/j.pt.2026.10.003. PMID- 42951721 OWN - NLM STAT- Publisher LR - 20261028 IS - 1558-4623 (Electronic) IS - 0001-2998 (Linking) DP - 2026 Oct 28 TI - Molecular imaging in adult spondylodiscitis: current evidence, clinical applications, and future perspectives. LID - S0001-2998(26)00192-3 [pii] LID - 10.1053/j.semnuclmed.2026.10.004 [doi] AB - Adult spondylodiscitis is an infection of the intervertebral disc and adjacent vertebral bodies that may progress to paravertebral extension, epidural involvement, spinal instability, sepsis, and neurological compromise. Because clinical symptoms and laboratory markers are often nonspecific, imaging is central to diagnosis, assessment of disease extent, biopsy planning, and selective evaluation of treatment response. Magnetic resonance imaging (MRI) remains the first-line anatomical technique for early marrow, disc, epidural, and paraspinal disease, while computed tomography (CT) is valuable for osseous destruction, instability, and biopsy guidance. Molecular imaging complements these modalities by depicting biological activity rather than structure alone. Fluorine-18 fluorodeoxyglucose positron emission tomography/computed tomography ([18F]FDG PET/CT) is the most established molecular technique, offering high diagnostic performance, whole-body evaluation for multifocal or metastatic infection, and support for management when MRI is contraindicated, equivocal, or difficult to interpret after surgery. Its main limitations are limited pathogen specificity, uptake in sterile inflammatory or reparative processes, heterogeneous interpretation criteria, and uncertain thresholds for treatment monitoring. Emerging approaches, including hybrid positron emission tomography/magnetic resonance imaging (PET/MRI), fibroblast activation protein inhibitor (FAPI) tracers, pathogen-targeted radiopharmaceuticals, quantitative PET biomarkers, and artificial intelligence (AI), remain investigational and require prospective validation of diagnostic and clinical utility. This review summarizes current evidence, clinical applications, limitations, and future directions for molecular imaging in spondylodiscitis. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Al-Ibraheem, Akram AU - Al-Ibraheem A AD - Department of Nuclear Medicine, King Hussein Cancer Center (KHCC), P.O. Box 1269, Al-Jubeiha, Amman 11941, Jordan; School of Medicine, University of Jordan, Queen Rania Street, Amman 11942, Jordan. Electronic address: aibraheem@khcc.jo. FAU - Estrada-Lobato, Enrique AU - Estrada-Lobato E AD - Nuclear Medicine and Diagnostic Imaging Section, Division of Human Health, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna International Centre, P.O. Box 100, 1400 Vienna, Austria. FAU - Ruzzeh, Saad AU - Ruzzeh S AD - Department of Nuclear Medicine, King Hussein Cancer Center (KHCC), P.O. Box 1269, Al-Jubeiha, Amman 11941, Jordan. FAU - Brink, Anita AU - Brink A AD - Nuclear Medicine and Diagnostic Imaging Section, Division of Human Health, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna International Centre, P.O. Box 100, 1400 Vienna, Austria. FAU - Giammarile, Francesco AU - Giammarile F AD - Nuclear Medicine and Diagnostic Imaging Section, Division of Human Health, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna International Centre, P.O. Box 100, 1400 Vienna, Austria. FAU - Al-Foqahaa, Mahed AU - Al-Foqahaa M AD - Department of Nuclear Medicine, King Hussein Cancer Center (KHCC), P.O. Box 1269, Al-Jubeiha, Amman 11941, Jordan. FAU - Al-Jaddadi, Maryam AU - Al-Jaddadi M AD - Department of Nuclear Medicine, King Hussein Cancer Center (KHCC), P.O. Box 1269, Al-Jubeiha, Amman 11941, Jordan. FAU - Hussain, Safa AU - Hussain S AD - Department of Nuclear Medicine, King Hussein Cancer Center (KHCC), P.O. Box 1269, Al-Jubeiha, Amman 11941, Jordan. FAU - Amro, Abdullah AU - Amro A AD - Department of Nuclear Medicine, King Hussein Cancer Center (KHCC), P.O. Box 1269, Al-Jubeiha, Amman 11941, Jordan. FAU - Paez, Diana AU - Paez D AD - Nuclear Medicine and Diagnostic Imaging Section, Division of Human Health, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna International Centre, P.O. Box 100, 1400 Vienna, Austria. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Semin Nucl Med JT - Seminars in nuclear medicine JID - 1264464 SB - IM OTO - NOTNLM OT - FDG PET/CT OT - Magnetic resonance imaging OT - Molecular imaging OT - Spinal infection OT - Spondylodiscitis OT - Vertebral osteomyelitis COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 23:01 PHST- 2026/10/10 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 23:01 [entrez] AID - S0001-2998(26)00192-3 [pii] AID - 10.1053/j.semnuclmed.2026.10.004 [doi] PST - aheadofprint SO - Semin Nucl Med. 2026 Oct 28:S0001-2998(26)00192-3. doi: 10.1053/j.semnuclmed.2026.10.004. PMID- 42951718 OWN - NLM STAT- Publisher LR - 20261028 IS - 1558-4623 (Electronic) IS - 0001-2998 (Linking) DP - 2026 Oct 28 TI - Imaging of myositis: An overview and update. LID - S0001-2998(26)00194-7 [pii] LID - 10.1053/j.semnuclmed.2026.10.007 [doi] AB - Idiopathic inflammatory myopathies (IIMs) are heterogeneous autoimmune disorders that affect skeletal muscle and may involve the skin, lungs, heart, gastrointestinal tract, or be associated with malignancy. Imaging supports phenotyping, biopsy targeting, assessment of disease burden, and monitoring. Magnetic resonance imaging (MRI) remains the principal modality for muscle assessment, depicting edema, fascial and subcutaneous inflammation, fatty replacement, and atrophy without ionizing radiation. Whole-body MRI maps disease distribution, whereas quantitative techniques, including Dixon fat-fraction imaging and T2 mapping, provide objective measures of activity and chronic damage. Ultrasound offers a portable, inexpensive adjunct, although operator dependence and limited standardization restrict comprehensive assessment. For nuclear medicine, 18F-fluorodeoxyglucose (FDG) PET/CT provides a complementary whole-body measure of metabolism, potentially identifying muscle inflammation, interstitial lung disease, other systemic manifestations, and occult malignancy in selected patients. Physiologic muscle uptake and heterogeneous acquisition and quantification protocols limit specificity and prevent universal PET thresholds. Hybrid PET/MRI combines metabolic and structural characterization, but evidence supporting routine use remains limited. Fibroblast activation protein inhibitor (FAPI) PET is an emerging pathway-targeted approach with preliminary evidence in muscle and myositis-associated lung disease. This review summarizes imaging patterns across major myositis phenotypes, contrasts established and investigational uses of MRI, ultrasound, FDG PET/CT, PET/MRI, and emerging tracers, illustrates important mimics and pitfalls, and proposes a practical multimodality imaging approach. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Dabiri, Mona AU - Dabiri M AD - Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, 601 North Caroline Street, Baltimore, MD 21287, United States. FAU - Ahlawat, Shivani AU - Ahlawat S AD - Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, 601 North Caroline Street, Baltimore, MD 21287, United States. FAU - Shaghaghi, Reza AU - Shaghaghi R AD - Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, 601 North Caroline Street, Baltimore, MD 21287, United States. FAU - Fayad, Laura M AU - Fayad LM AD - Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, 601 North Caroline Street, Baltimore, MD 21287, United States. Electronic address: lfayad1@jhmi.edu. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Semin Nucl Med JT - Seminars in nuclear medicine JID - 1264464 SB - IM OTO - NOTNLM OT - Dermatomyositis OT - FAPI OT - FDG PET/CT OT - Idiopathic inflammatory myopathy OT - MRI OT - PET/MRI OT - Ultrasound COIS- Declaration of competing interest The authors disclose the following research funding relationships: EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 23:01 PHST- 2026/10/18 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 23:01 [entrez] AID - S0001-2998(26)00194-7 [pii] AID - 10.1053/j.semnuclmed.2026.10.007 [doi] PST - aheadofprint SO - Semin Nucl Med. 2026 Oct 28:S0001-2998(26)00194-7. doi: 10.1053/j.semnuclmed.2026.10.007. PMID- 42951717 OWN - NLM STAT- Publisher LR - 20261028 IS - 1558-4623 (Electronic) IS - 0001-2998 (Linking) DP - 2026 Oct 29 TI - PSMA-directed radioligand therapy and radium-223 in prostate cancer bone metastases. LID - S0001-2998(26)00190-X [pii] LID - 10.1053/j.semnuclmed.2026.10.003 [doi] AB - Bone metastases are a major cause of pain, skeletal complications, marrow compromise and mortality in advanced prostate cancer. The therapeutic biology of skeletal disease is heterogeneous: malignant epithelial cells expressing prostate-specific membrane antigen (PSMA) coexist with osteoblasts, osteoclasts, reactive mineralising bone and variable marrow infiltration. Radium-223 dichloride is a bone-seeking alpha emitter with randomised evidence of overall-survival benefit in selected men with metastatic castration-resistant prostate cancer (mCRPC), whereas PSMA-targeted radioligand therapy delivers radiation to PSMA-expressing tumour deposits in bone and soft tissues. Their mechanistic complementarity has motivated sequential and concurrent strategies, but complementary localisation does not itself establish clinical synergy. This review critically integrates ALSYMPCA, ERA-223, PEACE-3, VISION, TheraP, PSMAfore, RALU and AlphaBet, together with radiobiology, molecular imaging, marrow and skeletal toxicity, dosimetry and evolving therapeutic sequencing. The final 133-patient retrospective RALU report describes the safety and outcomes observed after radium-223 followed by lutetium-177-PSMA in selected heavily pretreated patients; AlphaBet reports preliminary safety and antitumour activity with concurrent lutetium-177-PSMA-I&T and radium-223 in a single-arm early-phase study. Neither provides randomised evidence of an incremental survival benefit for dual-radionuclide treatment. Conversely, PEACE-3 establishes an overall-survival benefit for radium-223 combined with enzalutamide under contemporary bone-protective practice, whereas ERA-223 underscores the fracture hazards of inappropriate combinations. Future trials must integrate lesion-level imaging, whole-body disease phenotype, marrow reserve, patient-reported outcomes, competing systemic treatments and personalised radiation exposure. The most defensible clinical strategy is evidence-weighted selection of radionuclide therapy according to disease distribution and treatment history, with concurrent dual-radionuclide approaches restricted to appropriately governed trials. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Inc. All rights reserved. FAU - Sathekge, Mike AU - Sathekge M AD - Nuclear Medicine Research Infrastructure (NuMeRI), Pretoria, South Africa; Department of Nuclear Medicine, University of Pretoria, and Steve Biko Academic Hospital, Pretoria, South Africa. Electronic address: mike.sathekge@up.ac.za. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - United States TA - Semin Nucl Med JT - Seminars in nuclear medicine JID - 1264464 SB - IM OTO - NOTNLM OT - Bone metastases OT - Dosimetry OT - Metastatic castration-resistant prostate cancer OT - PSMA-targeted radioligand therapy OT - Radiobiology OT - Radium-223 COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 23:01 PHST- 2026/10/12 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 23:01 [entrez] AID - S0001-2998(26)00190-X [pii] AID - 10.1053/j.semnuclmed.2026.10.003 [doi] PST - aheadofprint SO - Semin Nucl Med. 2026 Oct 29:S0001-2998(26)00190-X. doi: 10.1053/j.semnuclmed.2026.10.003. PMID- 42951714 OWN - NLM STAT- Publisher LR - 20261028 IS - 1532-1967 (Electronic) IS - 0305-7372 (Linking) DP - 2026 Oct 29 TI - The evolving landscape of DLL3-directed Therapeutics in small cell lung cancer. PG - 103234 LID - S0305-7372(26)00148-9 [pii] LID - 10.1016/j.ctrv.2026.103234 [doi] AB - Small cell lung cancer (SCLC) remains one of the most lethal thoracic malignancies, characterized by rapid progression, early metastasis, and limited durable responses to conventional therapy. Delta-like ligand 3 (DLL3), a Notch pathway inhibitory ligand aberrantly overexpressed on the surface of approximately 70-85% of SCLC tumor cells but largely absent from normal adult tissue, has emerged as a compelling and therapeutically actionable target. Early efforts to exploit DLL3 through the antibody-drug conjugate (ADC) rovalpituzumab tesirine (Rova-T) yielded important mechanistic insights despite failing to demonstrate survival benefit in phase III trials and have since catalyzed a new generation of more refined DLL3-directed strategies. This review critically examines the evolving portfolio of DLL3-targeted therapies across three principal modalities: T-cell engagers, ADCs, and chimeric antigen receptor T-cell (CAR-T) therapies. In parallel, we examine emerging non-DLL3 therapeutic strategies reshaping the broader SCLC landscape. We additionally discuss the translational science underpinning DLL3 as a biomarker, including the emerging roles of circulating tumor cell-based liquid biopsy and DLL3-directed immunoPET-CT imaging in patient selection and treatment monitoring. CI - Copyright (c) 2026 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Tieu, Vivian Thuy AU - Tieu VT AD - Department of Internal Medicine, University of California Irvine School of Medicine, 101 The City Drive South, Orange, CA, 92868, United States. FAU - Arter, Zhaohui L AU - Arter ZL AD - Department of Internal Medicine, University of California Irvine School of Medicine, 101 The City Drive South, Orange, CA, 92868, United States. FAU - Nagasaka, Misako AU - Nagasaka M AD - Department of Internal Medicine, University of California Irvine School of Medicine, 101 The City Drive South, Orange, CA, 92868, United States; Department of Medicine, St. Marianna University School of Medicine, Kawasaki, JAPAN. Electronic address: nagasakm@hs.uci.edu. LA - eng PT - Journal Article PT - Review DEP - 20261029 PL - Netherlands TA - Cancer Treat Rev JT - Cancer treatment reviews JID - 7502030 SB - IM OTO - NOTNLM OT - ADC OT - BiTE OT - CAR-T OT - DLL3 OT - Notch signaling pathway OT - Small cell lung cancer COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Outside of the work reported in this paper: VT: nothing to declare. ZA: Advisory board for Catalyst, Janssen/JNJ., Rigel, EMD Serono, BI, AZ, Jassen. MN: Honoraria from AstraZeneca, Daiichi Sankyo, Lilly, Pfizer, Genentech, BMS/Mirati, Takeda, Johnson and Johnson, Boehringer Ingelheim, and Regeneron; consulting fees from Caris Life Sciences; travel support from AnHeart Therapeutics/ Nuvation Bio. The authors report no other potential conflicts of interest in this work. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 23:00 PHST- 2026/05/21 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 23:00 [entrez] AID - S0305-7372(26)00148-9 [pii] AID - 10.1016/j.ctrv.2026.103234 [doi] PST - aheadofprint SO - Cancer Treat Rev. 2026 Oct 29:103234. doi: 10.1016/j.ctrv.2026.103234. PMID- 42951702 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1943-7722 (Electronic) IS - 0002-9173 (Linking) VI - 166 IP - 4 DP - 2026 Oct 10 TI - Subclonal AGGF1::PDGFRB rearrangement in a myelodysplastic/myeloproliferative neoplasm with suboptimal response to imatinib. LID - aqag115 [pii] LID - 10.1093/ajcp/aqag115 [doi] AB - OBJECTIVES: Myeloid/lymphoid neoplasms with PDGFRB rearrangements are rare hematologic malignancies with marked clinicopathologic and genetic heterogeneity. AGGF1 is an exceptionally rare PDGFRB fusion partner previously reported only in pediatric lymphoblastic leukemia. Here, we describe the first AGGF1::PDGFRB rearrangement in a myeloid neoplasm and characterize its clonal evolution. METHODS: A man older than 80 years of age with progressive leukocytosis, anemia, and thrombocytopenia underwent bone marrow examination, conventional karyotyping, next-generation DNA sequencing, and RNA sequencing. RESULTS: The bone marrow showed features of a myelodysplastic/myeloproliferative neoplasm with neutrophilia. Molecular studies identified clonally dominant pathogenic DNMT3A, RUNX1, SF3B1, and WT1 mutations and KMT2A partial tandem duplication. RNA sequencing detected a subclonal AGGF1::PDGFRB fusion, supported by inv(5)(q13q33) in 6 of 20 metaphases. Hydroxyurea and imatinib produced a marked reduction in leukocyte count within 1 month. Two months later, however, the disease progressed to acute myeloid leukemia with acquisition of FLT3 internal tandem duplication, while AGGF1::PDGFRB was no longer detectable. CONCLUSIONS: This case expands the spectrum of PDGFRB-rearranged myeloid neoplasms and demonstrates that a potentially targetable tyrosine kinase rearrangement may arise as a subclonal rather than a founding event. Defining clonal architecture and temporal evolution is therefore important for accurate disease classification, therapy selection, and interpretation of treatment response. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of American Society for Clinical Pathology. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Monika, Fnu AU - Monika F AD - Department of Pathology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, United States. AD - Morsani College of Medicine, University of South Florida, Tampa, FL, United States. FAU - Shao, Haipeng AU - Shao H AD - Department of Pathology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, United States. FAU - Jain, Akriti G AU - Jain AG AD - Leukemia & Myeloid Disorders Program, Taussig Cancer Institute, Cleveland Clinic, Cleveland, OH, United States. FAU - Tackett, Michelle AU - Tackett M AD - Malignant Hematology Program, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, United States. FAU - Kuykendall, Andrew T AU - Kuykendall AT AD - Malignant Hematology Program, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, United States. FAU - Sallman, David A AU - Sallman DA AD - Malignant Hematology Program, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, United States. FAU - Yan, Mingfei AU - Yan M AUID- ORCID: 0000-0002-2054-5986 AD - Department of Pathology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, United States. LA - eng PT - Case Reports PT - Journal Article PL - England TA - Am J Clin Pathol JT - American journal of clinical pathology JID - 0370470 RN - EC 2.7.10.1 (Receptor, Platelet-Derived Growth Factor beta) RN - 8A1O1M485B (Imatinib Mesylate) RN - EC 2.7.10.1 (PDGFRB protein, human) RN - 0 (Antineoplastic Agents) RN - 0 (Oncogene Proteins, Fusion) SB - IM MH - Humans MH - *Receptor, Platelet-Derived Growth Factor beta/genetics MH - *Imatinib Mesylate/therapeutic use MH - Male MH - *Myelodysplastic-Myeloproliferative Diseases/genetics/pathology/drug therapy MH - Gene Rearrangement MH - Aged, 80 and over MH - Antineoplastic Agents/therapeutic use MH - *Oncogene Proteins, Fusion/genetics MH - *Myeloproliferative Disorders/genetics/pathology/drug therapy OTO - NOTNLM OT - AGGF1 OT - PDGFRB OT - clonal evolution OT - myeloid/lymphoid neoplasm EDAT- 2026/10/28 23:27 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 22:52 PHST- 2026/08/02 00:00 [received] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:52 [entrez] AID - 9004156 [pii] AID - 10.1093/ajcp/aqag115 [doi] PST - ppublish SO - Am J Clin Pathol. 2026 Oct 10;166(4):aqag115. doi: 10.1093/ajcp/aqag115. PMID- 42951697 OWN - NLM STAT- Publisher LR - 20261028 IS - 1469-7793 (Electronic) IS - 0022-3751 (Linking) DP - 2026 Oct 28 TI - Bacteroidota-derived glycine lipids differentially regulate skeletal muscle mitochondrial adaptation during Western diet feeding in male Ldlr(-/-) mice. LID - 10.1113/JP291898 [doi] AB - The Western diet (WD) is associated with skeletal muscle mitochondrial dysfunction, impaired oxidative metabolism, redox imbalance and inflammation. Emerging evidence suggests that gut microbiome-derived metabolites contribute to host metabolic regulation, yet the role of Bacteroidota-derived glycine lipids in skeletal muscle remains unknown. This study tested whether two structurally related bacterial glycine lipids, lipid 654 (L654) and lipid 1256 (L1256), regulate skeletal muscle mitochondrial remodelling in WD-fed Ldlr(-/-) mice. Male Ldlr(-/-) mice were fed a normal chow or WD and administered L654, L1256 or vehicle control for 8 weeks. Skeletal muscle glycine lipid accumulation, mitochondrial respiration, mitochondrial biogenesis markers, mitophagy-associated signalling, inflammatory and antioxidant proteins and muscle fibre cross-sectional area were assessed. L1256, but not L654, accumulated in skeletal muscle. Functionally, L1256 selectively enhanced fatty acid-supported mitochondrial respiration and increased respiratory control ratio. L1256 also increased peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1alpha), mitochondrial transcription factor A (TFAM) and electron transport chain complexes IV and V, suggesting enhanced oxidative mitochondrial remodelling. In parallel, L1256 altered markers associated with mitochondrial quality control signalling, increased nuclear factor erythroid 2-related factor 2 (Nrf2), manganese superoxide dismutase (MnSOD) and glutathione peroxidase 4 (GPX4), suppressed nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB) signalling and preserved muscle fibre cross-sectional area. L654 upregulated mitochondrial biogenesis transcripts without altering protein abundance. These findings identify L1256 as a microbiome-derived lipid retained within skeletal muscle that enhanced oxidative metabolism and preserved mitochondrial quality signalling under metabolic stress. The data suggest that tissue retention of bacterial lipids may be an important determinant of skeletal muscle mitochondrial adaptation. KEY POINTS: Western diet contributes to skeletal muscle mitochondrial dysfunction, impaired oxidative metabolism and chronic inflammation. Microbiome-derived glycine lipids are bioactive molecules from Bacteroidota species, but their effects on skeletal muscle mitochondrial metabolism remain poorly understood. Bacteroidota-derived glycine lipids produce distinct skeletal muscle responses in Western diet-fed Ldlr(-) (/) (-) mice, where lipid 1256 accumulated in skeletal muscle and enhanced fatty acid-supported respiration, whereas lipid 654 primarily induced transcriptional responses without detectable tissue accumulation. Lipid 1256 increased peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1alpha), mitochondrial transcription factor A (TFAM) and complexes IV and V of the electron transport chain together with restoration of Parkin, nuclear factor erythroid 2-related factor 2 (Nrf2), manganese superoxide dismutase (MnSOD) and glutathione peroxidase 4 (GPX4) while reducing nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB) and interleukin 6 (IL-6) expression and preserving skeletal muscle cross-sectional area. These findings suggest that gut microbiome-derived glycine lipids are regulators of skeletal muscle mitochondrial metabolism, and that tissue retention of bacterial lipids influences mitochondrial oxidative remodelling during metabolic stress. CI - (c) 2026 The Author(s). The Journal of Physiology (c) 2026 The Physiological Society. FAU - Kim, Siyun AU - Kim S AUID- ORCID: 0009-0004-5105-9755 AD - Department of Kinesiology, University of Connecticut, Storrs, Connecticut, USA. FAU - Noh, Sung Gi AU - Noh SG AD - Department of Kinesiology, University of Connecticut, Storrs, Connecticut, USA. FAU - Ji, Minje AU - Ji M AD - Department of Kinesiology, University of Connecticut, Storrs, Connecticut, USA. FAU - Vence, Tomas AU - Vence T AD - Department of Physiology and Neurobiology, University of Connecticut, Storrs, Connecticut, USA. FAU - Gao, Lidan AU - Gao L AUID- ORCID: 0009-0007-4096-4778 AD - Department of Nutritional Sciences, University of Connecticut, Storrs, Connecticut, USA. FAU - Otoko, Oliver AU - Otoko O AD - Department of Nutritional Sciences, University of Connecticut, Storrs, Connecticut, USA. FAU - Nichols, Frank C AU - Nichols FC AD - Department of Periodontology, University of Connecticut School of Dental Medicine, Farmington, Connecticut, USA. FAU - Blesso, Christopher N AU - Blesso CN AD - Department of Nutritional Sciences, University of Connecticut, Storrs, Connecticut, USA. FAU - Kwon, Oh Sung AU - Kwon OS AUID- ORCID: 0000-0003-2935-1130 AD - Department of Kinesiology, University of Connecticut, Storrs, Connecticut, USA. AD - UConn Center on Aging, University of Connecticut Health, Farmington, Connecticut, USA. AD - Department of Orthopedic Surgery, University of Connecticut Health, Farmington, Connecticut, USA. LA - eng GR - University of Connecticut/ PT - Journal Article DEP - 20261028 PL - England TA - J Physiol JT - The Journal of physiology JID - 0266262 SB - IM OTO - NOTNLM OT - Western diet OT - bacterial glycine lipids OT - inflammation OT - mitochondrial quality control OT - mitochondrial respiration OT - skeletal muscle EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 22:42 PHST- 2026/06/11 00:00 [received] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:42 [entrez] AID - 10.1113/JP291898 [doi] PST - aheadofprint SO - J Physiol. 2026 Oct 28. doi: 10.1113/JP291898. PMID- 42951695 OWN - NLM STAT- Publisher LR - 20261028 IS - 2508-5859 (Electronic) IS - 2508-5859 (Linking) DP - 2026 Oct 29 TI - Minimally invasive versus open liver resection for hepatocellular carcinoma in older adults: A systematic review and meta-analysis. LID - 10.14701/ahbps.26-167 [doi] AB - BACKGROUNDS/AIMS: As the population ages, an increasing number of older adults are diagnosed with resectable hepatocellular carcinoma (HCC). Although minimally invasive liver resection (MILR) has shown perioperative advantages, its safety and efficacy in older patients are not fully established. This meta-analysis evaluated the outcomes of MILR compared to open liver resection (OLR) in older adults (>/= 65 years) with HCC. METHODS: A search was conducted in Medline, Embase, CINAHL, and The Cochrane Library from their inception until November 20, 2025. The review adhered to PRISMA guidelines and was prospectively registered on PROSPERO (CRD420251239665). RESULTS: Fourteen retrospective studies comprising 3,092 older adults were included (1,367 MILR, 1,725 OLR). MILR was associated with lower overall (odds ratio [OR] = 0.47; 95% confidence interval [CI] 0.38-0.58; I(2) = 0%) and major complications (OR = 0.61; 95% CI 0.45-0.83; I(2) = 15%). However, no statistically significant difference was detected in 90-day mortality (OR = 0.75; 95% CI 0.41-1.38; I(2) = 0%), disease-free survival (hazard ratio [HR] = 0.83; 95% CI 0.60-1.15; I(2) = 72%), or overall survival (HR = 0.80; 95% CI 0.56-1.16; I(2) = 80%). CONCLUSIONS: In selected older adults with HCC, MILR was associated with fewer perioperative complications than OLR, while no statistically significant difference was detected in 90-day mortality or long-term survival. Residual confounding, predominance of minor resections, absence of validated frailty assessment, and limited octogenarian data preclude causal inference or confirmation that the association persists with advancing age. FAU - Al Darwashi, Lamees M AU - Al Darwashi LM AUID- ORCID: 0009-0004-8509-7725 AD - School of Medicine, University of Nicosia, Nicosia, Cyprus. FAU - Michael, Nicoleta AU - Michael N AUID- ORCID: 0009-0005-8415-1344 AD - School of Medicine, University of Nicosia, Nicosia, Cyprus. FAU - Kifle, Amanuel M AU - Kifle AM AUID- ORCID: 0009-0005-0891-6739 AD - School of Medicine, University of Nicosia, Nicosia, Cyprus. FAU - Elgazar, Sarah AU - Elgazar S AUID- ORCID: 0009-0001-9414-136X AD - School of Medicine, University of Nicosia, Nicosia, Cyprus. FAU - Faccenda, Alberto AU - Faccenda A AUID- ORCID: 0009-0000-9023-2814 AD - School of Medicine, University of Nicosia, Nicosia, Cyprus. FAU - Ghani, Ahmed AU - Ghani A AUID- ORCID: 0009-0002-4019-3612 AD - School of Medicine, University College Dublin, Dublin, Ireland. FAU - Alogakos, Marios AU - Alogakos M AUID- ORCID: 0009-0005-5625-7395 AD - Division of Surgical Oncology, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, United States. FAU - Than, Christian A AU - Than CA AUID- ORCID: 0000-0002-4193-9925 AD - Cambridge University Hospitals NHS Foundation Trust, Addenbrooke's Hospital, Cambridge, United Kingdom. AD - School of Biomedical Sciences, The University of Queensland, St Lucia, Australia. FAU - Nakanishi, Hayato AU - Nakanishi H AUID- ORCID: 0000-0002-2883-722X AD - Department of Surgery, Mayo Clinic, Rochester, MN, United States. FAU - Al Zadjali, Adil AU - Al Zadjali A AUID- ORCID: 0009-0005-8368-5598 AD - Department of Surgery, Sultan Qaboos University Hospital, University Medical City, Muscat, Sultanate of Oman. FAU - Al Farai, Abdallah AU - Al Farai A AUID- ORCID: 0000-0001-8330-2968 AD - Surgical Oncologist, GI Cancers Program, Sultan Qaboos Comprehensive Cancer Care & Research Centre (SQCCCRC), University Medical City, Muscat, Sultanate of Oman. LA - eng PT - Journal Article DEP - 20261029 PL - Korea (South) TA - Ann Hepatobiliary Pancreat Surg JT - Annals of hepato-biliary-pancreatic surgery JID - 101698342 OTO - NOTNLM OT - Carcinoma, hepatocellular OT - Hepatectomy OT - Minimally invasive surgical procedures EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 22:33 PHST- 2026/06/12 00:00 [received] PHST- 2026/08/18 00:00 [revised] PHST- 2026/08/30 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:33 [entrez] AID - ahbps.26-167 [pii] AID - 10.14701/ahbps.26-167 [doi] PST - aheadofprint SO - Ann Hepatobiliary Pancreat Surg. 2026 Oct 29. doi: 10.14701/ahbps.26-167. PMID- 42951694 OWN - NLM STAT- Publisher LR - 20261028 IS - 2508-5859 (Electronic) IS - 2508-5859 (Linking) DP - 2026 Oct 29 TI - Staple-line disruption of a short left-middle hepatic venous common trunk causing right atrial injury during extended left hepatectomy: A case report. LID - 10.14701/ahbps.26-219 [doi] AB - Injury to the inferior vena cava or the major hepatic veins is a life-threatening complication that can occur during hepatectomy. To prevent catastrophic outcomes, careful preoperative assessment of the venous anatomy, the selection of appropriate transection techniques, and advance coordination with cardiovascular surgical teams are essential. A 67-year-old male underwent an extended left hepatectomy with caudate lobectomy for intrahepatic cholangiocarcinoma that had invaded the roots of the left and middle hepatic veins. The common trunk of these veins was divided using a vascular stapler, and pulsatile bleeding developed from the staple-line approximately 4 minutes after transection. Despite immediate attempts at hemostasis, the staple-line disruption progressed; the laceration was ultimately found to extend from the hepatic venous confluence into the right atrium, resulting in uncontrollable hemorrhage. Cardiopulmonary bypass was established immediately, and complete hemostasis was achieved with primary repair. The patient recovered without major complications and remains alive 1 year after surgery. An extremely short left-middle hepatic venous common trunk located in close proximity to the right atrium may represent a technically challenging configuration for hepatic vein transection. Recognizing such anatomy preoperatively may encourage surgeons to consider alternative transection techniques and to plan vascular control strategies in advance for unexpected major venous bleeding. FAU - Nakahashi, Hiromitsu AU - Nakahashi H AUID- ORCID: 0009-0004-6419-0744 AD - Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, Institute of Medicine, University of Tsukuba, Tsukuba, Japan. FAU - Takahashi, Kazuhiro AU - Takahashi K AUID- ORCID: 0000-0003-1089-0644 AD - Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, Institute of Medicine, University of Tsukuba, Tsukuba, Japan. FAU - Tatsuzawa, Maiko AU - Tatsuzawa M AUID- ORCID: 0009-0005-3509-7302 AD - Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, Institute of Medicine, University of Tsukuba, Tsukuba, Japan. FAU - Furuta, Tomoaki AU - Furuta T AUID- ORCID: 0000-0001-8843-9605 AD - Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, Institute of Medicine, University of Tsukuba, Tsukuba, Japan. FAU - Miyazaki, Yoshihiro AU - Miyazaki Y AUID- ORCID: 0000-0002-1851-964X AD - Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, Institute of Medicine, University of Tsukuba, Tsukuba, Japan. FAU - Shimomura, Osamu AU - Shimomura O AUID- ORCID: 0000-0002-1735-5057 AD - Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, Institute of Medicine, University of Tsukuba, Tsukuba, Japan. FAU - Hashimoto, Shinji AU - Hashimoto S AUID- ORCID: 0009-0008-1362-4804 AD - Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, Institute of Medicine, University of Tsukuba, Tsukuba, Japan. FAU - Oda, Tatsuya AU - Oda T AUID- ORCID: 0000-0001-6115-0158 AD - Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery, Institute of Medicine, University of Tsukuba, Tsukuba, Japan. LA - eng PT - Case Reports PT - Journal Article DEP - 20261029 PL - Korea (South) TA - Ann Hepatobiliary Pancreat Surg JT - Annals of hepato-biliary-pancreatic surgery JID - 101698342 OTO - NOTNLM OT - Cardiopulmonary bypass OT - Hepatectomy OT - Hepatic veins OT - Surgical staplers EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 22:33 PHST- 2026/08/14 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:33 [entrez] AID - ahbps.26-219 [pii] AID - 10.14701/ahbps.26-219 [doi] PST - aheadofprint SO - Ann Hepatobiliary Pancreat Surg. 2026 Oct 29. doi: 10.14701/ahbps.26-219. PMID- 42951688 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1745-1701 (Electronic) IS - 0586-7614 (Print) IS - 0586-7614 (Linking) VI - 52 IP - 6 DP - 2026 Oct 27 TI - Clozapine Augmentation Strategies and Clinical Predictors of Prolonged Tertiary Hospitalization in Clozapine-Resistant Schizophrenia: a Retrospective Cohort Study. LID - 10.1093/schbul/sbag187 [doi] LID - sbag187 AB - BACKGROUND AND HYPOTHESIS: Clozapine-resistant schizophrenia (CRS) remains a major clinical challenge, with persistent symptoms and functional impairment despite adequate clozapine treatment. Real-world evidence is limited, and guidelines recommend a narrow set of augmentation strategies-aripiprazole (D2/3R partial agonist), amisulpride (D2/3R selective antagonist), and electroconvulsive therapy (ECT)-without clear guidance on selection or sequencing. This study examined augmentation choice and its association with length of stay (LOS) and symptomatic outcomes. STUDY DESIGN: We conducted a retrospective cohort study using BC Psychosis Program electronic records (2012-2025). Symptom severity and functioning scores (PANSS, CGI-S, GAPS, SOFAS) were extracted at admission and discharge. Multivariable Cox models assessed LOS for index and discharge augmentation strategies. ANCOVA compared symptom change across discharge strategies. A machine-learning model examined predictors of ECT versus pharmacological augmentation at discharge. STUDY RESULTS: Eighty-eight admissions were included. In adjusted index models, D2/3R selective antagonism was associated with shorter LOS than ECT (HR 2.50, 95% CI 1.35-4.62); partial agonism was not (HR 1.41, 95% CI 0.75-2.63). Partial agonist augmentation had the highest inefficacy-related switching. By discharge strategy, ECT was associated with greater reduction in positive symptoms than selective antagonism (MD -3.20, 95% CI -5.65 to -0.75) and partial agonism (MD -3.87, 95% CI -6.57 to -1.16). Greater impairment in psychosis and functioning on admission predicted ECT at discharge (AUC 0.74; balanced accuracy 0.72). CONCLUSIONS: D2/3R selective antagonism was the most resource-efficient strategy, while ECT produced greater improvement in positive symptoms. Incorporating baseline psychosis severity and functioning into measurement-based care may support more informed augmentation selection. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Maryland Psychiatric Research Center. FAU - Baldwin, Kaitlyn AU - Baldwin K AD - Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia, V6T1Z3, Canada. FAU - Honer, William G AU - Honer WG AD - Department of Psychiatry, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, V6T2A1, Canada. AD - BC Psychosis Program, Vancouver, British Columbia, V6T2A1, Canada. FAU - Shahbazi, Reza AU - Shahbazi R AD - Machine Learning Engineer based in San Francisco, San Francisco, CA, United States. FAU - Fredrikson, Diane AU - Fredrikson D AD - Department of Psychiatry, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, V6T2A1, Canada. FAU - White, Randall F AU - White RF AD - Department of Psychiatry, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, V6T2A1, Canada. AD - BC Psychosis Program, Vancouver, British Columbia, V6T2A1, Canada. FAU - Villa-Rodriguez, Fidel AU - Villa-Rodriguez F AUID- ORCID: 0000-0002-8273-8820 AD - Non-Invasive Neurostimulation Therapies Laboratory, Department of Psychiatry, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, V6T2A1, Canada. AD - School of Biomedical Engineering, Faculty of Applied Science | Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, V6T1Z3, Canada. FAU - Rafizadeh, Reza AU - Rafizadeh R AUID- ORCID: 0000-0002-4163-8241 AD - Department of Psychiatry, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, V6T2A1, Canada. AD - BC Psychosis Program, Vancouver, British Columbia, V6T2A1, Canada. LA - eng PT - Journal Article PL - United States TA - Schizophr Bull JT - Schizophrenia bulletin JID - 0236760 RN - J60AR2IKIC (Clozapine) RN - 0 (Antipsychotic Agents) RN - 8110R61I4U (Amisulpride) SB - IM MH - Humans MH - *Clozapine/administration & dosage/pharmacology MH - *Schizophrenia, Treatment-Resistant/therapy/drug therapy MH - Female MH - Retrospective Studies MH - *Antipsychotic Agents/administration & dosage/pharmacology MH - Male MH - *Electroconvulsive Therapy/statistics & numerical data MH - Adult MH - *Length of Stay/statistics & numerical data MH - Middle Aged MH - *Outcome Assessment, Health Care MH - *Amisulpride/administration & dosage/pharmacology MH - *Schizophrenia/therapy PMC - PMC13716498 OTO - NOTNLM OT - ECT OT - augmentation OT - clozapine OT - schizophrenia OT - treatment-resistance COIS- R.R. is a recipient of 2025 Michael Smith Health Research BC/BC Schizophrenia Society Foundation Health Professional-Investigator award. K.B. and R.S. have none to declare. W.G.H. has received consulting fees or sat on paid advisory boards for: Newron, Bausch Health, Boehringer Ingelheim, and AbbVie. D.F. has received consulting fees or sat on paid advisory boards for Bausch Health and also sits on the Board of Directors for EPI Canada. F.V.R. has received research funding from CIHR, Brain Canada, MSFHR, VCHRI, and the Weston Brain Institute, along with philanthropic support from the Seedlings Foundation and in-kind equipment support from MagVenture. He has received honoraria from Allergan and holds unpaid volunteer and committee roles with the BC Schizophrenia Society Foundation, Clinical TMS Society, and Brain Stimulation Society. R.F.W. has received income from Canadian Agency for Drugs and Technologies in Health and Advisory Board Activities for HLS Therapeutics. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 PMCR- 2026/10/28 CRDT- 2026/10/28 22:13 PHST- 2026/05/27 00:00 [received] PHST- 2026/08/09 00:00 [revised] PHST- 2026/08/26 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:13 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 9004153 [pii] AID - sbag187 [pii] AID - 10.1093/schbul/sbag187 [doi] PST - ppublish SO - Schizophr Bull. 2026 Oct 27;52(6):sbag187. doi: 10.1093/schbul/sbag187. PMID- 42951685 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1745-1701 (Electronic) IS - 0586-7614 (Print) IS - 0586-7614 (Linking) VI - 52 IP - 6 DP - 2026 Oct 27 TI - Risk Indicator Aggregation and Cluster Stability Across Developmental Domains from Early to Middle Childhood in Children at Familial High-Risk of Schizophrenia or Bipolar Disorder and Population-Based Controls. LID - 10.1093/schbul/sbag141 [doi] LID - sbag141 AB - BACKGROUND AND HYPOTHESES: Children of parents with schizophrenia or bipolar disorder are at increased risk of developing a mental disorder, including psychosis. While domain-specific developmental risk indicators have been identified, studies examining the accumulated risk distribution are lacking. Hypothesizing heterogeneity, we aimed to identify clusters with differential risk indicator load across known risk domains, and to examine cluster stability across early to middle childhood in children at familial high-risk of schizophrenia (FHR-SZ) or bipolar disorder (FHR-BP). STUDY DESIGN: A total of 441 children at FHR-SZ or FHR-BP and population-based controls were examined across multiple domains including neurocognition, social behavior, motor function, emotion regulation, language, and psychopathology at ages 7 and 11. Data from age 7 were clustered using k-means clustering and data from age 11 were clustered using the same cluster centers. STUDY RESULTS: Four clusters were identified and named Best outcome profile, Intermediate outcome profile, Selectively affected profile, and Broadly affected profile. The clusters showed marked heterogeneity regarding child performance and functioning across domains, and associations with family and caregiver functioning were shown across groups. From age 7 to 11, 60% stayed in the same cluster. CONCLUSIONS: Our findings of partially stable clusters with differential risk profiles for later onset of psychotic disorders evidence the need to consider risk heterogeneity in preventive interventions targeting offspring at FHR-SZ or FHR-BP, since some children have profiles with serious impairments across multiple domains while others have profiles with few or no impairments and therefore may be more resilient. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Maryland Psychiatric Research Center. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Krantz, Mette Falkenberg AU - Krantz MF AD - CORE- Copenhagen Research Center for Mental Health, Mental Health Center Copenhagen, Capital Region of Denmark, Copenhagen University Hospital, Gentofte Hospitalsvej, 2900 Hellerup, Denmark. AD - The Research Unit - Child and Adolescent Mental Health Center, Copenhagen University Hospital - Bispebjerg and Frederiksberg, Gentofte Hospitalsvej, 2900 Hellerup, Denmark. AD - The Lundbeck Foundation, 2100 Copenhagen O, Denmark. AD - Faculty of Health and Medical Sciences, University of Copenhagen - Institute of Clinical Medicine, 2200 Copenhagen N, Denmark. AD - Lundbeck Foundation Initiative for Integrative Psychiatric Research - iPSYCH, 8210 Aarhus V, Denmark. FAU - Hoffmann, Adam Gorm AU - Hoffmann AG AD - Department of Public Health, University of Copenhagen - Section of Biostatistics, 1353 Copenhagen K, Denmark. FAU - Hemager, Nicoline AU - Hemager N AUID- ORCID: 0000-0003-1339-9981 AD - CORE- Copenhagen Research Center for Mental Health, Mental Health Center Copenhagen, Capital Region of Denmark, Copenhagen University Hospital, Gentofte Hospitalsvej, 2900 Hellerup, Denmark. AD - The Research Unit - Child and Adolescent Mental Health Center, Copenhagen University Hospital - Bispebjerg and Frederiksberg, Gentofte Hospitalsvej, 2900 Hellerup, Denmark. AD - Lundbeck Foundation Initiative for Integrative Psychiatric Research - iPSYCH, 8210 Aarhus V, Denmark. AD - Department of Psychology, Faculty of Social Sciences, University of Copenhagen, 1353 Copenhagen K, Denmark. FAU - Gregersen, Maja AU - Gregersen M AUID- ORCID: 0000-0002-6510-1449 AD - CORE- Copenhagen Research Center for Mental Health, Mental Health Center Copenhagen, Capital Region of Denmark, Copenhagen University Hospital, Gentofte Hospitalsvej, 2900 Hellerup, Denmark. AD - The Research Unit - Child and Adolescent Mental Health Center, Copenhagen University Hospital - Bispebjerg and Frederiksberg, Gentofte Hospitalsvej, 2900 Hellerup, Denmark. AD - Lundbeck Foundation Initiative for Integrative Psychiatric Research - iPSYCH, 8210 Aarhus V, Denmark. FAU - Weye, Nanna AU - Weye N AD - CORE- Copenhagen Research Center for Mental Health, Mental Health Center Copenhagen, Capital Region of Denmark, Copenhagen University Hospital, Gentofte Hospitalsvej, 2900 Hellerup, Denmark. FAU - Sondergaard, Anne AU - Sondergaard A AD - CORE- Copenhagen Research Center for Mental Health, Mental Health Center Copenhagen, Capital Region of Denmark, Copenhagen University Hospital, Gentofte Hospitalsvej, 2900 Hellerup, Denmark. AD - Lundbeck Foundation Initiative for Integrative Psychiatric Research - iPSYCH, 8210 Aarhus V, Denmark. FAU - Burton, Birgitte AU - Burton B AD - CORE- Copenhagen Research Center for Mental Health, Mental Health Center Copenhagen, Capital Region of Denmark, Copenhagen University Hospital, Gentofte Hospitalsvej, 2900 Hellerup, Denmark. AD - Lundbeck Foundation Initiative for Integrative Psychiatric Research - iPSYCH, 8210 Aarhus V, Denmark. FAU - Brandt, Julie AU - Brandt J AD - CORE- Copenhagen Research Center for Mental Health, Mental Health Center Copenhagen, Capital Region of Denmark, Copenhagen University Hospital, Gentofte Hospitalsvej, 2900 Hellerup, Denmark. AD - Lundbeck Foundation Initiative for Integrative Psychiatric Research - iPSYCH, 8210 Aarhus V, Denmark. FAU - Greve, Aja AU - Greve A AUID- ORCID: 0000-0002-1369-6639 AD - Lundbeck Foundation Initiative for Integrative Psychiatric Research - iPSYCH, 8210 Aarhus V, Denmark. AD - The Psychosis Research Unit, Aarhus University Hospital - Psychiatry, 8200 Aarhus N, Denmark. AD - Center for Clinical Intervention and Neuropsychiatric Schizophrenia Research (CINS) and Center for Neuropsychiatric Schizophrenia Research (CNSR), Mental Health Centre Glostrup, University of Copenhagen, 2600 Glostrup, Denmark. FAU - Knudsen, Christina Bruun AU - Knudsen CB AD - Lundbeck Foundation Initiative for Integrative Psychiatric Research - iPSYCH, 8210 Aarhus V, Denmark. AD - The Psychosis Research Unit, Aarhus University Hospital - Psychiatry, 8200 Aarhus N, Denmark. FAU - Andreassen, Anna Krogh AU - Andreassen AK AD - Lundbeck Foundation Initiative for Integrative Psychiatric Research - iPSYCH, 8210 Aarhus V, Denmark. AD - The Psychosis Research Unit, Aarhus University Hospital - Psychiatry, 8200 Aarhus N, Denmark. FAU - Veddum, Lotte AU - Veddum L AUID- ORCID: 0000-0002-5235-2345 AD - Lundbeck Foundation Initiative for Integrative Psychiatric Research - iPSYCH, 8210 Aarhus V, Denmark. AD - The Psychosis Research Unit, Aarhus University Hospital - Psychiatry, 8200 Aarhus N, Denmark. AD - Department of Clinical Medicine, Faculty of Health and Medical Services, Aarhus University, 8200 Aarhus N, Denmark. FAU - Jepsen, Jens Richardt Mollegaard AU - Jepsen JRM AD - CORE- Copenhagen Research Center for Mental Health, Mental Health Center Copenhagen, Capital Region of Denmark, Copenhagen University Hospital, Gentofte Hospitalsvej, 2900 Hellerup, Denmark. AD - The Research Unit - Child and Adolescent Mental Health Center, Copenhagen University Hospital - Bispebjerg and Frederiksberg, Gentofte Hospitalsvej, 2900 Hellerup, Denmark. AD - Center for Clinical Intervention and Neuropsychiatric Schizophrenia Research (CINS) and Center for Neuropsychiatric Schizophrenia Research (CNSR), Mental Health Centre Glostrup, University of Copenhagen, 2600 Glostrup, Denmark. FAU - Mors, Ole AU - Mors O AD - The Psychosis Research Unit, Aarhus University Hospital - Psychiatry, 8200 Aarhus N, Denmark. AD - Department of Clinical Medicine, Faculty of Health and Medical Services, Aarhus University, 8200 Aarhus N, Denmark. FAU - Thorup, Anne A E AU - Thorup AAE AD - The Research Unit - Child and Adolescent Mental Health Center, Copenhagen University Hospital - Bispebjerg and Frederiksberg, Gentofte Hospitalsvej, 2900 Hellerup, Denmark. AD - Faculty of Health and Medical Sciences, University of Copenhagen - Institute of Clinical Medicine, 2200 Copenhagen N, Denmark. FAU - Ekstrom, Claus Thorn AU - Ekstrom CT AD - Department of Public Health, University of Copenhagen - Section of Biostatistics, 1353 Copenhagen K, Denmark. FAU - Nordentoft, Merete AU - Nordentoft M AD - CORE- Copenhagen Research Center for Mental Health, Mental Health Center Copenhagen, Capital Region of Denmark, Copenhagen University Hospital, Gentofte Hospitalsvej, 2900 Hellerup, Denmark. AD - Faculty of Health and Medical Sciences, University of Copenhagen - Institute of Clinical Medicine, 2200 Copenhagen N, Denmark. LA - eng GR - R102-A9118/Lundbeck Foundation Initiative for Integrative Psychiatric Research/ GR - R155-2014-1724/Lundbeck Foundation Initiative for Integrative Psychiatric Research/ GR - R381-2021-1305/Lundbeckfonden/ GR - Mental Health Services of the Capital Region of Denmark/ GR - Aarhus University/ GR - University of Copenhagen/ GR - Tryg Foundation/ GR - Innovation Fund/ GR - Beatrice Surovell Haskell Fund for Child Mental Health Research of Copenhagen/ PT - Journal Article PL - United States TA - Schizophr Bull JT - Schizophrenia bulletin JID - 0236760 SB - IM MH - Humans MH - Child MH - *Schizophrenia/epidemiology/physiopathology MH - Female MH - *Bipolar Disorder/physiopathology/epidemiology MH - Male MH - *Child of Impaired Parents/statistics & numerical data MH - Cluster Analysis MH - *Child Development/physiology MH - Clustering Algorithms MH - Risk PMC - PMC13716501 OTO - NOTNLM OT - cluster profiles OT - heterogeneity OT - offspring risk OT - prevention OT - scalability OT - severe mental illness COIS- The authors report no financial, personal, or commercial conflicts of interest and they take responsibility for the integrity and content of this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 PMCR- 2027/10/28 CRDT- 2026/10/28 22:13 PHST- 2026/01/21 00:00 [received] PHST- 2026/06/25 00:00 [revised] PHST- 2026/07/02 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:13 [entrez] PHST- 2027/10/28 00:00 [pmc-release] AID - 9004150 [pii] AID - sbag141 [pii] AID - 10.1093/schbul/sbag141 [doi] PST - ppublish SO - Schizophr Bull. 2026 Oct 27;52(6):sbag141. doi: 10.1093/schbul/sbag141. PMID- 42951684 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1745-1701 (Electronic) IS - 0586-7614 (Print) IS - 0586-7614 (Linking) VI - 52 IP - 6 DP - 2026 Oct 27 TI - Neurometabolic Differences in Psychosis Observed with 7 T Magnetic Resonance Spectroscopy. LID - 10.1093/schbul/sbag137 [doi] LID - sbag137 AB - BACKGROUND: Altered brain chemistry is thought to contribute to impaired cognition and perceptual functioning in people with psychotic psychopathology (PwPP). As heritable genetic factors shape the development of psychosis, alterations in brain chemistry may extend to biological relatives of PwPP. Magnetic resonance spectroscopy (MRS) is a non-invasive method for quantifying concentrations of various neurochemicals in the human brain. Previous studies using MRS have generally focused on specific diagnoses (eg, schizophrenia), rather than PwPP trans-diagnostically, and comparably few studies have examined relatives. The goal of the current study was to assess neurometabolic differences in PwPP and relatives using MRS in a large number of participants. STUDY DESIGN: As part of the Psychosis Human Connectome Project, we acquired ultra-short echo time (8 ms) MRS data at 7 tesla in both occipital and prefrontal cortices from 64 PwPP, 42 first-degree biological relatives, and 43 controls. STUDY RESULTS: We observed lower levels of N-acetyl-aspartate (NAA) in the occipital lobe in PwPP and relatives versus controls, and a trend toward lower N-acetyl-aspartylglutamate in prefrontal cortex in PwPP versus controls. Lower OCC NAA levels were also evident in diagnostic subgroups of PwPP (schizophrenia and bipolar disorder) compared to controls. We also observed higher levels of glucose in both occipital and prefrontal cortices in PwPP. Hierarchical clustering analyses showed that higher glucose levels were linked to higher psychiatric symptoms, poorer cognition, and impaired visual task performance. CONCLUSIONS: Together, our findings point to alterations in neural metabolism across multiple brain areas in PwPP that are associated with impaired cognitive and perceptual functioning. CI - Published by Oxford University Press on behalf of the Maryland Psychiatric Research Center 2026. FAU - Schallmo, Michael-Paul AU - Schallmo MP AUID- ORCID: 0000-0001-8252-8607 AD - Department of Psychiatry and Behavioral Sciences, University of Minnesota, Minneapolis, MN 55454, United States. FAU - Demro, Caroline AU - Demro C AUID- ORCID: 0000-0003-0620-9713 AD - Department of Psychiatry and Behavioral Sciences, University of Minnesota, Minneapolis, MN 55454, United States. AD - Department of Psychology, University of Minnesota, Minneapolis, MN 55455, United States. FAU - Killebrew, Kyle W AU - Killebrew KW AD - Department of Psychiatry and Behavioral Sciences, University of Minnesota, Minneapolis, MN 55454, United States. FAU - Sheldon, Aislin A AU - Sheldon AA AD - Department of Psychiatry and Behavioral Sciences, University of Minnesota, Minneapolis, MN 55454, United States. FAU - Olman, Cheryl A AU - Olman CA AD - Department of Psychology, University of Minnesota, Minneapolis, MN 55455, United States. AD - Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota, Minneapolis, MN 55455, United States. FAU - Sponheim, Scott R AU - Sponheim SR AUID- ORCID: 0000-0002-2782-0856 AD - Department of Psychiatry and Behavioral Sciences, University of Minnesota, Minneapolis, MN 55454, United States. AD - Veterans Affairs Medical Center, Minneapolis, MN 55417, United States. FAU - Marjanska, Malgorzata AU - Marjanska M AD - Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota, Minneapolis, MN 55455, United States. LA - eng GR - U01 MH108150/NH/NIH HHS/United States GR - P41 EB015894/University of Minnesota Center for Magnetic Resonance Research/ GR - P30 NS076408/University of Minnesota Center for Magnetic Resonance Research/ GR - UL1 TR002494/University of Minnesota Clinical and Translational Science Institute/ PT - Journal Article PL - United States TA - Schizophr Bull JT - Schizophrenia bulletin JID - 0236760 RN - 30KYC7MIAI (Aspartic Acid) RN - 997-55-7 (N-acetylaspartate) RN - 1W8M12WXYL (isospaglumic acid) RN - 0 (Dipeptides) SB - IM MH - Humans MH - Female MH - *Psychotic Disorders/metabolism/diagnostic imaging/physiopathology MH - *Aspartic Acid/analogs & derivatives/metabolism MH - Male MH - Adult MH - Magnetic Resonance Spectroscopy/methods MH - *Prefrontal Cortex/metabolism/diagnostic imaging MH - *Occipital Lobe/metabolism/diagnostic imaging MH - Connectome MH - Young Adult MH - *Schizophrenia/metabolism/diagnostic imaging MH - Middle Aged MH - Dipeptides PMC - PMC13716499 OTO - NOTNLM OT - N-acetyl-aspartate OT - bipolar disorder OT - glucose OT - human connectome project OT - magnetic resonance spectroscopy OT - schizophrenia COIS- The authors have no conflicts of interest to disclose with regard to this manuscript. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 PMCR- 2026/10/28 CRDT- 2026/10/28 22:13 PHST- 2026/01/27 00:00 [received] PHST- 2026/05/29 00:00 [revised] PHST- 2026/06/25 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:13 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 9004149 [pii] AID - sbag137 [pii] AID - 10.1093/schbul/sbag137 [doi] PST - ppublish SO - Schizophr Bull. 2026 Oct 27;52(6):sbag137. doi: 10.1093/schbul/sbag137. PMID- 42951659 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 0485-1439 (Print) IS - 0485-1439 (Linking) VI - 67 IP - 9 DP - 2026 TI - [Fundamentals of genetic counseling in hematology: pedigree construction and management of secondary findings in HemeSight((R))]. PG - 1399-1407 LID - 10.11406/rinketsu.67.1399 [doi] AB - Since the clinical adoption of comprehensive genomic profiling for hematologic malignancies (HemeSight((R))) in 2025, hematologists have increasingly encountered germline findings in routine practice. In hematologic malignancies, genetic information is relevant not only to diagnosis, prognosis, and treatment selection, but also to the clinical management of at-risk relatives-including predictive testing- and donor selection for hematopoietic stem cell transplantation. This review summarizes the fundamentals of genetic counseling in hematology, focusing on the characteristics of genetic information, the distinction and relationship between genetic medicine and genetic counseling, the significance of family history-taking and pedigree construction, and the management of secondary findings identified through HemeSight((R)). In addition, two hypothetical cases are presented: a case of AML with a pathogenic germline DDX41 variant and a case of pediatric ALL in which a pathogenic germline BRCA1 variant was identified as a secondary finding. These cases highlight practical issues, including predictive testing for relatives, evaluation of related donor candidates, and the need for multidisciplinary collaboration. In hematology practice, timely collaboration with clinical genetics services is essential for supporting patients and their families. FAU - Akiyama, Nana AU - Akiyama N AD - Department of Clinical Genomics, The University of Tokyo Hospital. LA - jpn PT - Case Reports PT - English Abstract PT - Journal Article PT - Review PL - Japan TA - Rinsho Ketsueki JT - [Rinsho ketsueki] The Japanese journal of clinical hematology JID - 2984782R RN - EC 3.6.4.13 (DEAD-box RNA Helicases) RN - 0 (BRCA1 Protein) SB - IM MH - Humans MH - *Genetic Counseling/methods MH - *Pedigree MH - Female MH - *Hematologic Neoplasms/genetics/therapy/diagnosis MH - Male MH - Genetic Testing MH - DEAD-box RNA Helicases/genetics MH - Germ-Line Mutation MH - BRCA1 Protein/genetics OTO - NOTNLM OT - Family tree OT - Genetic counseling OT - Germline findings OT - Predictive testing EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 22:03 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:03 [entrez] AID - 10.11406/rinketsu.67.1399 [doi] PST - ppublish SO - Rinsho Ketsueki. 2026;67(9):1399-1407. doi: 10.11406/rinketsu.67.1399. PMID- 42951653 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 0485-1439 (Print) IS - 0485-1439 (Linking) VI - 67 IP - 9 DP - 2026 TI - [Management of invasive fungal infections in hematologic malignancies: practical antifungal selection considering drug-drug interactions]. PG - 1351-1358 LID - 10.11406/rinketsu.67.1351 [doi] AB - Recent advances in molecularly targeted therapies have substantially shifted the treatment landscape of hematologic malignancies. Nevertheless, invasive fungal infections remain a major complication, particularly in the setting of prolonged neutropenia and profound immunosuppression, and continue to affect both incidence and clinical outcomes, necessitating appropriate management. In recent years, drug-drug interactions between triazole antifungals and molecularly targeted or immunosuppressive agents have been shown to influence not only pharmacokinetics and adverse events but also treatment continuity. Consequently, antifungal selection is no longer limited to infection control but has become an integral component of overall treatment strategy. Focusing on acute myeloid leukemia and hematopoietic cell transplantation, this review summarizes the importance of appropriate antifungal selection in clinical practice based on the risk of fungal infections and the impact of drug-drug interactions in the era of novel therapies. FAU - Mori, Yasuo AU - Mori Y AD - Department of Hematology, Oncology & Cardiovascular Medicine Kyushu University Hospital. LA - jpn PT - English Abstract PT - Journal Article PT - Review PL - Japan TA - Rinsho Ketsueki JT - [Rinsho ketsueki] The Japanese journal of clinical hematology JID - 2984782R RN - 0 (Antifungal Agents) RN - 0 (Immunosuppressive Agents) RN - 0 (Triazoles) SB - IM MH - Humans MH - *Antifungal Agents/therapeutic use/adverse effects/pharmacokinetics MH - Drug Interactions MH - *Hematologic Neoplasms/complications/therapy MH - *Invasive Fungal Infections/drug therapy/etiology MH - Immunosuppressive Agents/adverse effects/therapeutic use MH - Triazoles/therapeutic use/adverse effects MH - Molecular Targeted Therapy OTO - NOTNLM OT - Drug-drug interaction OT - Fungal infection OT - Molecularly targeted agent OT - Triazole EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 22:03 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:03 [entrez] AID - 10.11406/rinketsu.67.1351 [doi] PST - ppublish SO - Rinsho Ketsueki. 2026;67(9):1351-1358. doi: 10.11406/rinketsu.67.1351. PMID- 42951647 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 0485-1439 (Print) IS - 0485-1439 (Linking) VI - 67 IP - 9 DP - 2026 TI - [Impact of genomic panel testing on indications for transplantation in hematological malignancies]. PG - 1304-1309 LID - 10.11406/rinketsu.67.1304 [doi] AB - Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is an important curative treatment for hematologic malignancies, but its indications are determined by prognosis and treatment response. With the widespread adoption of cancer gene panel testing using next-generation sequencing, refined prognostic models integrating genomic information-such as the ELN 2022 classification for acute myeloid leukemia and the IPSS-M for myelodysplastic syndromes-are now being introduced into clinical practice. This enables more comprehensive risk stratification than is possible with conventional clinical and morphological indicators alone, refining indications for transplantation. In acute lymphoblastic leukemia, the identification of poor-prognosis genetic abnormalities, such as Ph-like ALL, also contributes to transplant decision-making during the first complete remission. Furthermore, the detection of germline pathogenic variants, notably DDX41 mutations, has crucial implications for related donor selection and genetic counseling. This review outlines the latest findings and future perspectives regarding the impact of gene panel testing on transplant indications and donor selection in hematologic malignancies. FAU - Kanda, Junya AU - Kanda J AD - Department of Hematology, Graduate School of Medicine, Kyoto University. LA - jpn PT - English Abstract PT - Journal Article PT - Review PL - Japan TA - Rinsho Ketsueki JT - [Rinsho ketsueki] The Japanese journal of clinical hematology JID - 2984782R SB - IM MH - Humans MH - *Hematologic Neoplasms/genetics/therapy/diagnosis MH - *Hematopoietic Stem Cell Transplantation MH - *Genetic Testing/methods MH - High-Throughput Nucleotide Sequencing OTO - NOTNLM OT - Allogeneic hematopoietic stem cell transplantation OT - Germline predisposition OT - Hematologic malignancies OT - Next-generation sequencing EDAT- 2026/10/28 23:27 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 22:03 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:03 [entrez] AID - 10.11406/rinketsu.67.1304 [doi] PST - ppublish SO - Rinsho Ketsueki. 2026;67(9):1304-1309. doi: 10.11406/rinketsu.67.1304. PMID- 42951642 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 0485-1439 (Print) IS - 0485-1439 (Linking) VI - 67 IP - 9 DP - 2026 TI - [T-cell redirecting therapies for multiple myeloma: an update]. PG - 1257-1270 LID - 10.11406/rinketsu.67.1257 [doi] AB - T-cell redirecting therapies, particularly BCMA-directed CAR-T-cell therapy and bispecific antibodies, have substantially advanced the treatment of multiple myeloma. These therapies induce deep responses in patients with relapsed or refractory disease after exposure to proteasome inhibitors, immunomodulatory drugs, and anti-CD38 antibodies, and are increasingly being used in earlier lines of therapy. However, the availability of multiple BCMA-targeted approaches, including CAR-T-cell therapy, bispecific antibodies, and belantamab mafodotin, has made treatment selection increasingly complex. In clinical practice, it is important to consider not only efficacy, but also treatment sequence, bridging therapy before CAR-T-cell infusion, effects on subsequent therapies, treatment access, and patient-specific risks. In addition, GPRC5D-targeted therapy and dual-target strategies may provide new options for patients previously treated with BCMA-targeted therapies and for those with extramedullary disease. Long-term management, including infection prevention, hypogammaglobulinemia, prolonged cytopenias, and secondary malignancies, is also becoming increasingly important. This review summarizes the current clinical role of T-cell redirecting therapies in relapsed or refractory multiple myeloma, with a focus on treatment sequencing, extramedullary disease, long-term management, and future directions. FAU - Yoshihara, Kyoko AU - Yoshihara K AD - Department of Hematology, Hyogo Medical University. AD - Department of Transfusion and Cellular Therapy, Hyogo Medical University. LA - jpn PT - English Abstract PT - Journal Article PT - Review PL - Japan TA - Rinsho Ketsueki JT - [Rinsho ketsueki] The Japanese journal of clinical hematology JID - 2984782R RN - 0 (Antibodies, Bispecific) RN - 0 (Antibodies, Monoclonal, Humanized) RN - 0 (B-Cell Maturation Antigen) RN - DB1041CXDG (belantamab mafodotin) RN - 0 (Receptors, Chimeric Antigen) SB - IM MH - Humans MH - *Multiple Myeloma/therapy/immunology MH - Antibodies, Bispecific/therapeutic use MH - *Immunotherapy, Adoptive/methods MH - *T-Lymphocytes/immunology MH - Antibodies, Monoclonal, Humanized/therapeutic use MH - B-Cell Maturation Antigen/immunology MH - Molecular Targeted Therapy MH - Receptors, Chimeric Antigen OTO - NOTNLM OT - BCMA OT - Bispecific antibodies OT - CAR-T OT - Multiple myeloma EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 22:03 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:03 [entrez] AID - 10.11406/rinketsu.67.1257 [doi] PST - ppublish SO - Rinsho Ketsueki. 2026;67(9):1257-1270. doi: 10.11406/rinketsu.67.1257. PMID- 42951637 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 0485-1439 (Print) IS - 0485-1439 (Linking) VI - 67 IP - 9 DP - 2026 TI - [Diagnosis and management of peripheral T-cell lymphoma]. PG - 1217-1226 LID - 10.11406/rinketsu.67.1217 [doi] AB - Peripheral T-cell lymphoma (PTCL) comprises more than 30 distinct disease entities and represents a rare and heterogeneous group of lymphoid neoplasms. The 2022 WHO Classification of Haematolymphoid Tumours, 5th edition (WHO-HAEM5) and the International Consensus Classification (ICC) substantially refined the classification of nodal PTCL, most notably through the reorganization of TFH-phenotype nodal T-cell lymphomas into nodal T-follicular helper cell lymphoma (nTFHL) and the recognition of EBV-positive nodal T/NK-cell lymphoma as a distinct entity. In the first-line setting, brentuximab vedotin plus CHP (BV-CHP) demonstrated superiority over CHOP in the first positive phase III trial for CD30-positive PTCL, whereas no regimen has yet proven superior to CHOP for CD30-negative PTCL. The role of consolidative autologous stem cell transplantation in first remission remains insufficiently validated by prospective randomized trials, and JCOG2210 is currently underway in Japan to address this. In relapsed and refractory disease, no standard salvage regimen has been established and optimizing treatment selection based on molecular and clinical profiles remains a critical challenge. Accurate pathological diagnosis and molecular stratification will be central to future treatment development in PTCL. FAU - Suehara, Yasuhito AU - Suehara Y AD - Department of Hematology, Institute of Medicine, University of Tsukuba. LA - jpn PT - English Abstract PT - Journal Article PT - Review PL - Japan TA - Rinsho Ketsueki JT - [Rinsho ketsueki] The Japanese journal of clinical hematology JID - 2984782R RN - 7XL5ISS668 (Brentuximab Vedotin) SB - IM MH - Humans MH - *Lymphoma, T-Cell, Peripheral/diagnosis/therapy/classification MH - Antineoplastic Combined Chemotherapy Protocols/therapeutic use MH - Brentuximab Vedotin/administration & dosage OTO - NOTNLM OT - Anaplastic large cell lymphoma OT - Brentuximab vedotin OT - Nodal T-follicular helper cell lymphoma OT - Peripheral T-cell lymphoma EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 22:03 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:03 [entrez] AID - 10.11406/rinketsu.67.1217 [doi] PST - ppublish SO - Rinsho Ketsueki. 2026;67(9):1217-1226. doi: 10.11406/rinketsu.67.1217. PMID- 42951632 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 0485-1439 (Print) IS - 0485-1439 (Linking) VI - 67 IP - 9 DP - 2026 TI - [RNA helicases and hematopoietic neoplasms: insights into RNA processing and disease pathogenesis]. PG - 1172-1181 LID - 10.11406/rinketsu.67.1172 [doi] AB - RNA helicases comprise a large family of enzymes that act on RNA or RNA-protein complexes to remodel their structures. More than 70 RNA helicases have been identified in humans, and they are involved in nearly all cellular processes that require dynamic RNA structural rearrangements. A subset of myeloid neoplasms is associated with germline predisposition, among which DDX41, a representative member of the DEAD-box RNA helicase family, is one of the most important causative genes. Although one of the major cellular functions of DDX41 is thought to be pre-mRNA splicing, it is unlikely to directly determine splice site selection. Rather, recent studies suggest that DDX41 plays an integrative regulatory role in transcription and RNA splicing by preventing the accumulation of specific nucleic acid structures known as R-loops. This review summarizes the physiological roles of RNA helicases, including DDX41, and discusses their relevance to human disease, with a particular focus on hematopoietic neoplasms. FAU - Matsui, Hirotaka AU - Matsui H AD - Department of Laboratory Medicine, National Cancer Center Hospital. LA - jpn PT - English Abstract PT - Journal Article PT - Review PL - Japan TA - Rinsho Ketsueki JT - [Rinsho ketsueki] The Japanese journal of clinical hematology JID - 2984782R RN - EC 3.6.4.13 (DEAD-box RNA Helicases) RN - EC 3.6.1.- (DDX41 protein, human) RN - EC 3.6.4.13 (RNA Helicases) RN - 63231-63-0 (RNA) SB - IM MH - Humans MH - *DEAD-box RNA Helicases/genetics/physiology/metabolism MH - *Hematologic Neoplasms/genetics MH - RNA Splicing/genetics MH - *RNA Helicases/genetics/physiology MH - R-Loop Structures/genetics MH - RNA/metabolism/genetics OTO - NOTNLM OT - DDX41 OT - Myeloid neoplasms OT - R-loop OT - RNA helicase EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 22:03 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:03 [entrez] AID - 10.11406/rinketsu.67.1172 [doi] PST - ppublish SO - Rinsho Ketsueki. 2026;67(9):1172-1181. doi: 10.11406/rinketsu.67.1172. PMID- 42951631 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 0485-1439 (Print) IS - 0485-1439 (Linking) VI - 67 IP - 9 DP - 2026 TI - [Treatment strategies for chronic-phase chronic myeloid leukemia in 2026]. PG - 1159-1171 LID - 10.11406/rinketsu.67.1159 [doi] AB - The prognosis of chronic-phase chronic myeloid leukemia (CP-CML) has improved substantially since the introduction of tyrosine kinase inhibitor (TKI) therapy. Accordingly, treatment strategies now extend beyond disease control to include improving quality of life and achieving treatment-free remission (TFR). This review discusses first-line treatment selection based on the EUTOS long-term survival (ELTS) score, patient age, and the risk of vascular adverse events; the efficacy and safety profiles of second-generation TKIs, ponatinib, and asciminib; molecular response milestones according to treatment goals; and current approaches to TKI dose optimization. Furthermore, it summarizes treatment selection in the second-line and later settings based on BCR::ABL1 mutation analysis in patients with resistant disease, as well as the results of TFR clinical trials conducted in Japan. Drawing on this evidence, this review presents practical treatment strategies for CP-CML tailored to patient characteristics and therapeutic goals. FAU - Ono, Takaaki AU - Ono T AD - Department of Transfusion and Cell Therapy, Hamamatsu University School of Medicine. LA - jpn PT - English Abstract PT - Journal Article PT - Review PL - Japan TA - Rinsho Ketsueki JT - [Rinsho ketsueki] The Japanese journal of clinical hematology JID - 2984782R RN - 0 (Protein Kinase Inhibitors) RN - 0 (Pyridazines) RN - 4340891KFS (ponatinib) RN - EC 2.7.10.2 (Fusion Proteins, bcr-abl) RN - 0 (Antineoplastic Agents) RN - 0 (Imidazoles) SB - IM MH - Humans MH - *Protein Kinase Inhibitors/therapeutic use/administration & dosage/adverse effects MH - *Leukemia, Myeloid, Chronic-Phase/drug therapy/genetics MH - Pyridazines/administration & dosage/therapeutic use MH - Fusion Proteins, bcr-abl/genetics MH - Antineoplastic Agents MH - Imidazoles/administration & dosage/adverse effects/therapeutic use MH - Quality of Life OTO - NOTNLM OT - Asciminib OT - Chronic myeloid leukemia OT - Treatment-free remission OT - Tyrosine kinase inhibitor EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 22:03 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:03 [entrez] AID - 10.11406/rinketsu.67.1159 [doi] PST - ppublish SO - Rinsho Ketsueki. 2026;67(9):1159-1171. doi: 10.11406/rinketsu.67.1159. PMID- 42951624 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 0485-1439 (Print) IS - 0485-1439 (Linking) VI - 67 IP - 9 DP - 2026 TI - [Allogeneic hematopoietic stem cell transplantation in aplastic anemia: evolution of transplant strategies and updates to the 2024 ASTCT and 2026 ASH guidelines]. PG - 1103-1113 LID - 10.11406/rinketsu.67.1103 [doi] AB - Outcomes of immunosuppressive therapy (IST) for severe aplastic anemia (SAA) have improved with the addition of thrombopoietin receptor agonists; however, approximately one-third of patients still require allogeneic hematopoietic cell transplantation (HCT) due to treatment resistance or relapse. The 2024 ASTCT guidelines removed the historical age limit of 40 years for upfront matched sibling donor (MSD)-HCT and recommended matched unrelated donor (MUD)-HCT or haploidentical donor HCT over IST for patients without an MSD. The 2026 ASH guidelines adopt a more cautious approach but nonetheless explicitly prioritize MSD-HCT over IST for patients up to age 40 and recommend MUD-HCT as equivalent to IST. Both guidelines thus reflect a broader expansion of upfront transplant indications in SAA. This review examines the historical development of conditioning regimens, donor selection strategies including umbilical cord blood, and updated transplant indications in light of these evolving recommendations. FAU - Onishi, Yasushi AU - Onishi Y AD - Sendai Minami Onishi Clinic. AD - Department of Hematology, Tohoku University School of Medicine. LA - jpn PT - English Abstract PT - Journal Article PT - Review PL - Japan TA - Rinsho Ketsueki JT - [Rinsho ketsueki] The Japanese journal of clinical hematology JID - 2984782R RN - 0 (Immunosuppressive Agents) SB - IM MH - Humans MH - *Anemia, Aplastic/therapy MH - *Hematopoietic Stem Cell Transplantation/methods MH - Transplantation Conditioning/methods MH - *Practice Guidelines as Topic MH - Transplantation, Homologous MH - Immunosuppressive Agents/therapeutic use OTO - NOTNLM OT - Cord blood OT - Fulminant aplastic anemia OT - Haploidentical donor OT - Posttransplantation-cyclophosphamide EDAT- 2026/10/28 23:27 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 22:03 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:03 [entrez] AID - 10.11406/rinketsu.67.1103 [doi] PST - ppublish SO - Rinsho Ketsueki. 2026;67(9):1103-1113. doi: 10.11406/rinketsu.67.1103. PMID- 42951622 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 0485-1439 (Print) IS - 0485-1439 (Linking) VI - 67 IP - 9 DP - 2026 TI - [Molecular genetics of paroxysmal nocturnal hemoglobinuria]. PG - 1086-1092 LID - 10.11406/rinketsu.67.1086 [doi] AB - Paroxysmal nocturnal hemoglobinuria (PNH) is a clonal hematopoietic disorder caused by mutations affecting the biosynthesis of glycosylphosphatidylinositol (GPI) anchors. Recent advances in genetic analyses have shown that, in addition to PIGA mutations, rare mutations in PIGT, PIGB, and PIGV can also underlie PNH. Moreover, PNH may have an oligoclonal structure, and small PIGA-mutated clones can be detected even in healthy individuals. The expansion of PNH clones is thought to be driven not only by immune-mediated selective pressure but also, in some cases, by HMGA2 mutations and myeloid driver lesions, suggesting that PNH develops through multifactorial mechanisms that vary among patients. In addition, the genetic factors involved in progression to secondary myeloid neoplasms and response to complement inhibitors are becoming better understood. This article reviews the genetic findings relevant to PNH and discusses their clinical significance. FAU - Takamori, Hiroyuki AU - Takamori H AD - Department of Hematology and Oncology, Graduate School of Medicine, The University of Osaka. AD - Department of Hematology, Yao Municipal Hospital. LA - jpn PT - English Abstract PT - Journal Article PT - Review PL - Japan TA - Rinsho Ketsueki JT - [Rinsho ketsueki] The Japanese journal of clinical hematology JID - 2984782R RN - 0 (phosphatidylinositol glycan-class A protein) RN - 0 (Membrane Proteins) RN - 0 (Glycosylphosphatidylinositols) SB - IM MH - *Hemoglobinuria, Paroxysmal/genetics/immunology MH - Humans MH - *Mutation MH - Membrane Proteins/genetics MH - Glycosylphosphatidylinositols/biosynthesis OTO - NOTNLM OT - Bone marrow failure OT - Complement inhibitor OT - PIGA OT - Paroxysmal nocturnal hemoglobinuria EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 22:03 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:03 [entrez] AID - 10.11406/rinketsu.67.1086 [doi] PST - ppublish SO - Rinsho Ketsueki. 2026;67(9):1086-1092. doi: 10.11406/rinketsu.67.1086. PMID- 42951619 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 0485-1439 (Print) IS - 0485-1439 (Linking) VI - 67 IP - 9 DP - 2026 TI - [Dysregulated control of hematopoietic stem cells in myeloid malignancies]. PG - 1061-1068 LID - 10.11406/rinketsu.67.1061 [doi] AB - Myeloid neoplasia comprises a spectrum of disorders now recognized as a continuum, ranging from clonal hematopoiesis to overt disease. Recent genomic studies have shown that hematopoietic stem cells (HSCs) harboring somatic mutations undergo clonal expansion long before clinical manifestation. Although these mutant stem cells often retain multilineage differentiation potential, their intrinsic functions-including self-renewal capacity and differentiation dynamics-are altered. In addition, accumulating evidence indicates that differentiated progeny derived from mutant stem cells exhibit functional abnormalities, such as dysregulated inflammatory responses and altered interactions with the bone marrow microenvironment. These alterations, spanning both stem cell and differentiated cell compartments, contribute to inflammatory conditions, remodeling of the bone marrow niche, and the establishment of a selective environment that promotes clonal expansion while impairing normal hematopoiesis. In this educational review, I summarize recent advances in understanding dysregulated HSC control in the early stages of myeloid neoplasia, with particular emphasis on aging, inflammation, immune interactions, and crosstalk with the bone marrow niche. Focusing on these early events underscores the importance of viewing myeloid neoplasia from its cellular and microenvironmental origins. FAU - Yamashita, Masayuki AU - Yamashita M AD - Division of Experimental Hematology, Department of Hematology, St. Jude Children's Research Hospital. LA - jpn PT - English Abstract PT - Journal Article PT - Review PL - Japan TA - Rinsho Ketsueki JT - [Rinsho ketsueki] The Japanese journal of clinical hematology JID - 2984782R SB - IM MH - Humans MH - *Hematopoietic Stem Cells/pathology/cytology MH - Stem Cell Niche MH - Cell Differentiation/genetics MH - Animals MH - Hematopoiesis/genetics MH - Mutation MH - Aging OTO - NOTNLM OT - Bone marrow niche remodeling OT - Clonal hematopoiesis OT - Hematopoietic stem cell dysregulation OT - Myeloid neoplasia EDAT- 2026/10/28 23:27 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 22:03 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:03 [entrez] AID - 10.11406/rinketsu.67.1061 [doi] PST - ppublish SO - Rinsho Ketsueki. 2026;67(9):1061-1068. doi: 10.11406/rinketsu.67.1061. PMID- 42951617 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2095-8137 (Print) IS - 2095-8137 (Linking) VI - 47 IP - 6 DP - 2026 Nov 18 TI - Genome-wide scan and fine-mapping nominate ANAPC4 as a candidate modulator of Chinese Holstein heifer puberty timing. PG - 1969-1979 LID - 2095-8137(2026)06-1969-11 [pii] LID - 10.24272/j.issn.2095-8137.2025.462 [doi] AB - Dairy fertility strongly affects farm sustainability because young cows must be raised for many months before they begin producing milk. Age at first service (AFS) is a key driver of reproductive efficiency and rearing costs in dairy systems. We analyzed Chinese Holstein heifers to dissect the genetic architecture of AFS and nominate functional candidates. After low-coverage whole-genome sequencing and genotype imputation, a total of 7 964 217 SNPs were retained for genome-wide association study (GWAS) and window-based variance partitioning. Among the seven traits evaluated-AFS, age at first calving (AFC), first-to-last insemination interval in heifers (IFLh), number of services per conception in heifers (NSh), conception rate in heifers (CRh), birth weight of heifers (BiWh), and gestation length in heifers (GLh)-AFS exhibited the highest heritability, with a moderate estimate of h (2)=0.276. Three candidate regions prioritized by significance and additive variance jointly explained 0.91% of the genetic variance. On BTA6, the association peak spans ZCCHC4 and ANAPC4; notably, ANAPC4 contributes to estrogen-induced oocyte maturation. In blood, cis-expression quantitative trait loci mapping and co-localization analysis provided strong evidence that the GWAS locus colocalizes with ANAPC4 expression, with the posterior probability of shared causality (PP.H (4)) reaching 0.921. SuSiE fine-mapping pinpointed the association to a ~30 kb region comprising a 20 variant credible set that segregates into two high linkage disequilibrium haplotypes (H0 and H1), with the H1H1 genotype linked to elevated ANAPC4 expression and a ~2.1 day reduction in AFS. Motif analyses suggested allele-specific TF-binding differences between haplotypes consistent with regulatory effects. Taken together, these findings pinpoint a fine-mapped regulatory haplotype that upregulates ANAPC4 expression and is associated with earlier AFS, providing actionable markers for genomic selection to advance heifer puberty timing and improve farm profitability. FAU - Pan, Cui-Li AU - Pan CL AD - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, China. FAU - Ma, Long-Gang AU - Ma LG AD - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, China. FAU - Cao, Xue-Sha AU - Cao XS AD - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, China. FAU - Wang, Qi-Yan AU - Wang QY AD - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, China. FAU - Wang, Lu-Lu AU - Wang LL AD - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, China. FAU - Wang, Jiong AU - Wang J AD - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, China. FAU - Wang, Ao AU - Wang A AD - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, China. FAU - Li, Ran AU - Li R AD - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, China. FAU - Wang, Xi-Hong AU - Wang XH AD - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, China. FAU - Ma, Yun AU - Ma Y AD - College of Animal Science and Technology, Ningxia Key Laboratory of Ruminant Molecular and Cellular Breeding, Ningxia University, Yinchuan, Ningxia 750021, China. FAU - Liu, Guang-Lei AU - Liu GL AD - Hebei Pinyuan Biotechnology Co., Ltd., Shijiazhuang, Hebei 050600, China. FAU - Zhu, Kai AU - Zhu K AD - Hebei Pinyuan Biotechnology Co., Ltd., Shijiazhuang, Hebei 050600, China. FAU - Lv, Xin-Zhe AU - Lv XZ AD - Hebei Pinyuan Biotechnology Co., Ltd., Shijiazhuang, Hebei 050600, China. FAU - Zhang, Zhuang-Biao AU - Zhang ZB AD - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, China. E-mail: zhangzhuangbiao18@163.com. FAU - Jiang, Yu AU - Jiang Y AD - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi 712100, China. E-mail: yu.jiang@nwafu.edu.cn. LA - eng PT - Journal Article PL - China TA - Zool Res JT - Zoological research JID - 101697192 SB - IM MH - Animals MH - Cattle/genetics/physiology MH - Female MH - *Genome-Wide Association Study/veterinary MH - Polymorphism, Single Nucleotide MH - *Sexual Maturation/genetics/physiology MH - Quantitative Trait Loci MH - Chromosome Mapping OTO - NOTNLM OT - ANAPC4 OT - Chinese Holstein heifers OT - Fine-mapping OT - GWAS OT - Puberty timing EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 22:03 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:03 [entrez] AID - 2095-8137(2026)06-1969-11 [pii] AID - 10.24272/j.issn.2095-8137.2025.462 [doi] PST - ppublish SO - Zool Res. 2026 Nov 18;47(6):1969-1979. doi: 10.24272/j.issn.2095-8137.2025.462. PMID- 42951616 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2095-8137 (Print) IS - 2095-8137 (Linking) VI - 47 IP - 6 DP - 2026 Nov 18 TI - Neural representation of color-selective populations in the pigeon entopallium. PG - 1957-1968 LID - 2095-8137(2026)06-1957-12 [pii] LID - 10.24272/j.issn.2095-8137.2025.568 [doi] AB - Understanding how birds encode object color is fundamental to elucidating the neural mechanisms of color vision. Although behavioral studies have shown high color-discrimination accuracy in birds, it remains unclear whether higher-order visual areas organize color information in a structured manner. Here, we performed multi-site multi-unit activity recordings from the pigeon entopallium while presenting multi-category images that systematically varied in hue, luminance, and saturation. Many entopallium sites exhibited robust hue selectivity that remained stable across luminance and saturation changes, and population responses were shaped mainly by gain modulation. These sites also showed category-invariant color selectivity within the tested object set. Population-level analyses revealed a distinct and structured representational geometry that corresponded closely to perceptual color axes, with color-related correlations stronger than category-related correlations. These results suggest that the pigeon entopallium exhibits population-level color coding and produces a hue-related representational geometry that is broadly consistent with reports from primate V4 under comparable stimulus manipulations. FAU - He, Qing-Zhi AU - He QZ AD - School of Electrical and Information Engineering, Zhengzhou University, Zhengzhou, Henan 450001, China. FAU - Zhu, Jun-Cai AU - Zhu JC AD - School of Electrical and Information Engineering, Zhengzhou University, Zhengzhou, Henan 450001, China. FAU - Niu, Xiao-Ke AU - Niu XK AD - School of Electrical and Information Engineering, Zhengzhou University, Zhengzhou, Henan 450001, China. FAU - Wu, Peng AU - Wu P AD - School of Electrical and Information Engineering, Zhengzhou University, Zhengzhou, Henan 450001, China. FAU - Zhang, Ru-Peng AU - Zhang RP AD - School of Electrical and Information Engineering, Zhengzhou University, Zhengzhou, Henan 450001, China. FAU - Li, Bo AU - Li B AD - School of Electrical and Information Engineering, Zhengzhou University, Zhengzhou, Henan 450001, China. FAU - Wang, Jiang-Tao AU - Wang JT AD - School of Electrical and Information Engineering, Zhengzhou University, Zhengzhou, Henan 450001, China. E-mail: jiangtaowang@zzu.edu.cn. FAU - Wang, Zhi-Zhong AU - Wang ZZ AD - School of Electrical and Information Engineering, Zhengzhou University, Zhengzhou, Henan 450001, China. E-mail: wzz1982@zzu.edu.cn. LA - eng PT - Journal Article PL - China TA - Zool Res JT - Zoological research JID - 101697192 SB - IM MH - Animals MH - *Columbidae/physiology MH - *Color Perception/physiology MH - *Neurons/physiology MH - Color OTO - NOTNLM OT - Color representation OT - Entopallium OT - Hue selectivity OT - Neural coding OT - Pigeon EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 22:03 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:03 [entrez] AID - 2095-8137(2026)06-1957-12 [pii] AID - 10.24272/j.issn.2095-8137.2025.568 [doi] PST - ppublish SO - Zool Res. 2026 Nov 18;47(6):1957-1968. doi: 10.24272/j.issn.2095-8137.2025.568. PMID- 42951610 OWN - NLM STAT- Publisher LR - 20261028 IS - 1881-784X (Electronic) IS - 1881-7831 (Linking) DP - 2026 Oct 29 TI - Hot-water extraction, ethanol precipitation, and silkworm bioassays detect innate immune-stimulating activity in coffee extracts. LID - 10.5582/ddt.2026.01061 [doi] AB - A workflow combining hot-water extraction and ethanol precipitation can provide macromolecule-enriched fractions, but rapid in vivo evaluation of their innate immune-stimulating activity is not well established. We used coffee as a test matrix and evaluated the resulting fractions using a silkworm muscle-contraction assay and a Pseudomonas aeruginosa infection assay. Ethanol-precipitated fractions were prepared from three coffee origins, with or without lactic acid bacterial fermentation, under light- or dark-roast conditions. All sample groups showed measurable muscle-contraction activity. Specific activity ranged from 0.74 to 9.1 U/mg and was higher after light roasting in 11 of 12 matched conditions. Selected fractions increased survival at approximately 24 h after P. aeruginosa challenge, most clearly among Colombia EX samples; protection varied with bean origin, fermentation condition, roast, and dose. Non-fermented fractions were also active, indicating that fermentation was not uniformly required. These results support the feasibility of combining hot-water extraction, ethanol precipitation, and silkworm testing to detect bioactivity in macromolecule-enriched fractions. The method may be useful for prioritizing samples for chemical characterization. FAU - Miyashita, Atsushi AU - Miyashita A AD - Teikyo University Institute of Medical Mycology, Tokyo, Japan. FAU - Mikami, Kazuhiro AU - Mikami K AD - Teikyo University Institute of Medical Mycology, Tokyo, Japan. FAU - Tabuchi, Fumiaki AU - Tabuchi F AD - Teikyo University Institute of Medical Mycology, Tokyo, Japan. FAU - Sekimizu, Kazuhisa AU - Sekimizu K AD - Faculty of Pharma-Science, Teikyo University, Tokyo, Japan. AD - Genome Pharmaceutical Institute Co., Ltd., Tokyo, Japan. LA - eng PT - Journal Article DEP - 20261029 PL - Japan TA - Drug Discov Ther JT - Drug discoveries & therapeutics JID - 101493809 SB - IM OTO - NOTNLM OT - Bombyx mori OT - Pseudomonas aeruginosa OT - polysaccharide OT - prophylaxis EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 22:03 PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:03 [entrez] AID - 10.5582/ddt.2026.01061 [doi] PST - aheadofprint SO - Drug Discov Ther. 2026 Oct 29. doi: 10.5582/ddt.2026.01061. PMID- 42951607 OWN - NLM STAT- Publisher LR - 20261028 IS - 1349-3329 (Electronic) IS - 0040-8727 (Linking) DP - 2026 Oct 29 TI - EIF5A2 Promotes the Migration and Invasion of Esophageal Squamous Cell Carcinoma Cells and Tumor Progression in vivo Through GLUT2-Mediated Glycolysis. LID - 10.1620/tjem.2026.J118 [doi] FAU - Chen, Yushan AU - Chen Y AD - Radiology Department, Zhangzhou Affiliated Hospital of Fujian Medical University. FAU - Huang, Lingling AU - Huang L AD - Radiology Department, Zhangzhou Affiliated Hospital of Fujian Medical University. FAU - Zhan, Shujun AU - Zhan S AD - Radiology Department, Zhangzhou Affiliated Hospital of Fujian Medical University. FAU - Huang, Danqi AU - Huang D AD - Nuclear Medicine Department, Zhangzhou Affiliated Hospital of Fujian Medical University. FAU - Lin, Yong AU - Lin Y AD - Thoracic Surgery Department, Zhangzhou Affiliated Hospital of Fujian Medical University. FAU - Chen, Shaoqiang AU - Chen S AD - Department of Human Anatomy, Fujian Medical University. LA - eng PT - Journal Article DEP - 20261029 PL - Japan TA - Tohoku J Exp Med JT - The Tohoku journal of experimental medicine JID - 0417355 SB - IM EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 22:03 PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:03 [entrez] AID - 10.1620/tjem.2026.J118 [doi] PST - aheadofprint SO - Tohoku J Exp Med. 2026 Oct 29. doi: 10.1620/tjem.2026.J118. PMID- 42951603 OWN - NLM STAT- Publisher LR - 20261028 IS - 1557-7716 (Electronic) IS - 1523-0864 (Linking) DP - 2026 Oct 28 TI - The Major Iron Transporter DMT1 Promotes Renal Fibrosis by Activating Ferroptosis and Enforcing TGF-beta1 Signaling in Renal Tubular Cells. PG - 15230864261501810 LID - 10.1177/15230864261501810 [doi] AB - INTRODUCTION: Renal fibrosis is a common pathological feature of chronic kidney disease (CKD), but effective therapies are still lacking. Ferroptosis has been increasingly associated with CKD; however, the iron-handling proteins that link ferroptotic stress to fibrotic signaling remain poorly defined. Here, we identify the divalent metal transporter 1 (DMT1) as a previously unrecognized regulatory factor that integrates iron metabolism, ferroptotic stress, and transforming growth factor-beta (TGF-beta) signaling in tubular epithelial cells (TECs). RESULTS: DMT1 expression was elevated in TECs from folic acid injection- or unilateral ureteral obstruction-induced mouse CKD models and in isolated primary TECs exposed to TGF-beta1 or palmitic acid, accompanied by increased mitochondrial reactive oxygen species (mtROS) production. Pharmacological inhibition or genetic deletion of DMT1 in TECs significantly attenuated renal fibrosis, with reduced renal fibrosis area, decreased expression of profibrotic genes and markers, lower mtROS production, and restored fatty acid oxidation. DMT1 deficiency also attenuated lipid peroxidation, with reduced 4-HNE levels and lipid droplet deposition in mouse CKD models. Additionally, DMT1 deletion reduced tissue iron content, reversed ferroptosis-associated morphological changes in TECs, and restored tissue glutathione peroxidase-4 expression. Notably, DMT1 deletion reduced both total Smad2 and phosphorylated Smad2 induced by TGF-beta1, consistent with suppression of the TGF-beta1-Smad axis. INNOVATION AND CONCLUSIONS: These findings support a model in which tubular DMT1 integrates iron overload, ferroptotic stress, and TGF-beta1-Smad signaling during fibrotic progression in CKD. DMT1 functions as a signaling hub contributing to TGF-beta1-Smad axis activation, although a direct causal chain from iron influx to transforming growth factor beta receptor 1 induction remains to be fully delineated. DMT1 inhibition may offer a tubular-selective therapeutic strategy for CKD. Antioxid. Redox Signal. 00, 000-000. FAU - Pan, Ying AU - Pan Y AD - Department of Gastroenterology, Affiliated Hospital of Chifeng University, Chifeng, Inner Mongolia Autonomous Region, China. FAU - Liu, Meiqi AU - Liu M AD - Department of Gastroenterology, Affiliated Hospital of Chifeng University, Chifeng, Inner Mongolia Autonomous Region, China. FAU - Qiu, Jiawei AU - Qiu J AD - Department of Gastroenterology, Affiliated Hospital of Chifeng University, Chifeng, Inner Mongolia Autonomous Region, China. FAU - Jia, Liqin AU - Jia L AD - Department of Gastroenterology, Affiliated Hospital of Chifeng University, Chifeng, Inner Mongolia Autonomous Region, China. FAU - Li, Mingyang AU - Li M AD - Department of Gastroenterology, Affiliated Hospital of Chifeng University, Chifeng, Inner Mongolia Autonomous Region, China. FAU - Gong, Xiaohui AU - Gong X AD - Department of Emergency Medicine, Affiliated Hospital of Chifeng University, Chifeng, Inner Mongolia Autonomous Region, China. AD - Institute of Cardiovascular Disease of Chifeng University, Chifeng, Inner Mongolia Autonomous Region, China. FAU - Cao, Hongfei AU - Cao H AD - Department of Gastroenterology, Affiliated Hospital of Chifeng University, Chifeng, Inner Mongolia Autonomous Region, China. FAU - Cao, Hongru AU - Cao H AUID- ORCID: 0009-0006-8864-2287 AD - Department of Nephrology, Affiliated Hospital of Chifeng University, Chifeng, Inner Mongolia Autonomous Region, China. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Antioxid Redox Signal JT - Antioxidants & redox signaling JID - 100888899 SB - IM OTO - NOTNLM OT - DMT1 OT - TGF-beta1 OT - chronic kidney disease OT - ferroptosis OT - renal tubular cell EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 22:03 PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:03 [entrez] AID - 10.1177/15230864261501810 [doi] PST - aheadofprint SO - Antioxid Redox Signal. 2026 Oct 28:15230864261501810. doi: 10.1177/15230864261501810. PMID- 42951602 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1347-5223 (Electronic) IS - 0009-2363 (Linking) VI - 74 IP - 10 DP - 2026 TI - Stick-Slip Behavior of Ethenzamide-Based Binary Powder Mixtures Evaluated Using the Shear Cell Method: phi(CSL) as a Robust State Parameter. PG - 819-831 LID - 10.1248/cpb.c26-00420 [doi] AB - Stick-slip (SS) behavior often disrupts the smooth flow of pharmaceutical powders during transfer and can impair product uniformity. In this study, binary powder systems consisting of ethenzamide and pharmaceutical excipients were investigated using a constant-volume shear cell method to clarify the factors governing SS behavior in mixed powder formulations. Eight pharmaceutical excipients with distinct particle properties were selected to analyze the effects on SS behavior as a function of the mixing ratio. X-ray computed tomography (XRCT) was used to characterize interparticle void structures in excipient powder beds. The SS amplitude and interval increased with increasing consolidation load. The addition of excipients progressively suppressed the SS behavior depending on their void characteristics. Although limited to discrete mixing ratios and static XRCT, excipients with a higher fraction of interparticle voids of moderate size (5.6-23 microm) tended to suppress SS behavior at lower mixing ratios. Correlation analyses revealed strong positive correlations between the SS amplitude and static resistance parameters, such as the angle of internal friction and cohesion, whereas correlations with steady-state flow parameters were weak when data across different consolidation loads were analyzed together. At a fixed consolidation load, SS amplitude showed a clearer correlation with the dynamic friction angle (phi(CSL)), indicating that phi(CSL) reflects a flow-related property of the powder bed at the critical state and shows relatively robust behavior against variations in consolidation conditions. FAU - Miyazaki, Tamaki AU - Miyazaki T AD - Division of Drugs, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki 210-9501, Japan. FAU - Koide, Tatsuo AU - Koide T AD - Division of Drugs, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki 210-9501, Japan. FAU - Yonemochi, Etsuo AU - Yonemochi E AD - School of Pharmacy at Narita, International University of Health and Welfare, 4-3 Kozunomori, Narita, Chiba 286-8686, Japan. LA - eng PT - Journal Article PL - Japan TA - Chem Pharm Bull (Tokyo) JT - Chemical & pharmaceutical bulletin JID - 0377775 RN - 0 (Powders) RN - 0 (Excipients) RN - L929ZCK4BF (ethenzamide) RN - 0 (Salicylamides) SB - IM MH - Powders/chemistry MH - *Excipients/chemistry MH - Particle Size MH - Drug Compounding MH - Tomography, X-Ray Computed MH - Surface Properties MH - Salicylamides OTO - NOTNLM OT - ethenzamide OT - pharmaceutical excipient OT - powder flowability parameter OT - powder mixture OT - shear cell method OT - stick-slip phenomenon EDAT- 2026/10/28 23:27 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 22:03 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:03 [entrez] AID - 10.1248/cpb.c26-00420 [doi] PST - ppublish SO - Chem Pharm Bull (Tokyo). 2026;74(10):819-831. doi: 10.1248/cpb.c26-00420. PMID- 42951589 OWN - NLM STAT- Publisher LR - 20261028 IS - 1550-9613 (Electronic) IS - 0278-4297 (Linking) DP - 2026 Oct 28 TI - Doppler Ultrasonography and Computed Tomography Angiography in Carotid Artery Stenosis: Discordance and Its Potential Impact on Surgical Decision-Making. LID - 10.1002/jum.70530 [doi] AB - OBJECTIVES: The degree of carotid artery stenosis influences surgical decision-making; therefore, accurate assessment is essential for appropriate patient selection. This study evaluated agreement between Doppler ultrasonography (DUS) and computed tomography angiography (CTA) in patients with significant stenosis detected on DUS and investigated factors associated with discordance. METHODS: In this retrospective study, 79 patients who underwent DUS between January 2024 and October 2025 were included. DUS stenosis grade, CTA stenosis according to NASCET and ECST methods, lumen diameters, plaque characteristics, and accompanying vascular features were evaluated. RESULTS: Agreement between DUS and CTA was fair using NASCET (kappa = 0.413; p < .001) and moderate using ECST (kappa = 0.531; p < .001). Agreement between NASCET and ECST was substantial (kappa = 0.799; p < .001). Discordance was most frequent in the 50-69% DUS stenosis group and varied according to stenosis severity and CTA measurement method. Plaque type, plaque length, and calcification were not associated with discordance. Residual lumen diameter on CTA was significantly larger in NASCET-based discordant cases (p = .018) whereas no significant difference was observed using ECST classification. In some patients, discrepancies could potentially affect surgical decision-making. CONCLUSION: Agreement between DUS and CTA in significant carotid stenosis was fair using NASCET and moderate using ECST, with discordance particularly frequent in the 50-69% stenosis group and dependent on the CTA measurement method. Combined assessment with 2 noninvasive imaging modalities may help reduce misclassification in patients considered for surgical treatment. CI - (c) 2026 American Institute of Ultrasound in Medicine. FAU - Bayraktar, Ezgi Suat AU - Bayraktar ES AUID- ORCID: 0000-0003-0541-984X AD - Department of Radiology, Aydin State Hospital, Aydin, Turkey. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - J Ultrasound Med JT - Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine JID - 8211547 SB - IM OTO - NOTNLM OT - Doppler ultrasound OT - carotid stenosis OT - computed tomography angiography EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 22:03 PHST- 2026/10/01 00:00 [revised] PHST- 2026/08/26 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:03 [entrez] AID - 10.1002/jum.70530 [doi] PST - aheadofprint SO - J Ultrasound Med. 2026 Oct 28. doi: 10.1002/jum.70530. PMID- 42951585 OWN - NLM STAT- Publisher LR - 20261028 IS - 1349-7235 (Electronic) IS - 0918-2918 (Linking) DP - 2026 Oct 29 TI - Trough Rivaroxaban Plasma Concentrations and Early Thrombus Regression in Acute Pulmonary Embolism: A Sub-analysis of the SAKURA-PE/DVT REGISTRY. LID - 10.2169/internalmedicine.8146-26 [doi] AB - Objectives　To evaluate the association between trough rivaroxaban plasma concentrations and early thrombus regression in patients with acute pulmonary embolism (PE).Methods　This study was a sub-analysis of the multicenter, prospective SAKURA PE/DVT REGISTRY. The thrombus regression rate was calculated using contrast-enhanced computed tomography performed at diagnosis and approximately two weeks after treatment initiation. Trough rivaroxaban plasma concentrations were measured using a chromogenic anti-factor Xa assay. Correlations between plasma concentrations, thrombus regression rates, and coagulation parameters were analyzed.Patients　Among 153 patients with acute PE treated with direct oral anticoagulants, 106 received rivaroxaban, and trough plasma concentrations were measured in 51 patients.Results　The median trough rivaroxaban plasma concentration was 120.0 (55.6-186.0) ng/mL, with substantial interindividual variability. Trough rivaroxaban plasma concentrations were positively correlated with the thrombus regression rate in all patients (rho = 0.33, P = 0.018) and in the intensive therapy group (rho = 0.41, P = 0.009). In the multivariable analysis, log-transformed rivaroxaban plasma concentrations were independently associated with the thrombus regression rate in the intensive therapy group (beta = 9.79, 95% confidence interval 0.72-18.86; P = 0.035). Prothrombin time was strongly correlated with rivaroxaban plasma concentration (rho = 0.85, P < 0.001). Plasma concentrations tended to increase with age and showed greater variability in elderly patients.Conclusion　Higher trough rivaroxaban plasma concentrations were associated with greater early thrombus regression in patients with acute PE. Prothrombin time may be a practical surrogate for rivaroxaban exposure and may support individualized anticoagulation strategies in selected patients. FAU - Migita, Shohei AU - Migita S AD - Division of Cardiology, Department of Medicine, Nihon University Itabashi Hospital, Nihon University School of Medicine, Japan. FAU - Fukamachi, Daisuke AU - Fukamachi D AD - Department of Cardiology, Nihon University Hospital, Japan. FAU - Saito, Yuki AU - Saito Y AD - Division of Cardiology, Department of Medicine, Nihon University Itabashi Hospital, Nihon University School of Medicine, Japan. FAU - Toyama, Kazuto AU - Toyama K AD - Division of Cardiology, Department of Medicine, Nihon University Itabashi Hospital, Nihon University School of Medicine, Japan. FAU - Matsumoto, Naoya AU - Matsumoto N AD - Department of Cardiology, Nihon University Hospital, Japan. FAU - Okubo, Kimie AU - Okubo K AD - Itabashi Medical Association Hospital, Japan. FAU - Tachibana, Eizo AU - Tachibana E AD - Kawaguchi Municipal Medical Center, Japan. FAU - Oiwa, Koji AU - Oiwa K AD - Yokohama Chuo Hospital, Japan. FAU - Haruta, Hironori AU - Haruta H AD - TMG Asaka Medical Center, Japan. FAU - Nomoto, Kazumiki AU - Nomoto K AD - Tokyo Rinkai Hospital, Japan. FAU - Arima, Ken AU - Arima K AD - Kasukabe Municipal Medical Center, Japan. FAU - Ichikawa, Makoto AU - Ichikawa M AD - Sekishindo Hospital, Japan. FAU - Uchiyama, Hiroe AU - Uchiyama H AD - Department of Radiology, Nihon University School of Medicine, Japan. FAU - Tago, Kenichiro AU - Tago K AD - Department of Radiology, Nihon University School of Medicine, Japan. FAU - Okada, Masahiro AU - Okada M AD - Department of Radiology, Nihon University School of Medicine, Japan. FAU - Nakayama, Tomohiro AU - Nakayama T AD - Divisions of Laboratory Medicine, Department of Pathology and Microbiology, Nihon University School of Medicine, Japan. FAU - Okumura, Yasuo AU - Okumura Y AD - Division of Cardiology, Department of Medicine, Nihon University Itabashi Hospital, Nihon University School of Medicine, Japan. LA - eng PT - Journal Article DEP - 20261029 PL - Japan TA - Intern Med JT - Internal medicine (Tokyo, Japan) JID - 9204241 SB - IM OTO - NOTNLM OT - DOAC OT - anticoagulant OT - pulmonary embolism OT - rivaroxaban OT - venous thromboembolism EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 22:02 PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:02 [entrez] AID - 10.2169/internalmedicine.8146-26 [doi] PST - aheadofprint SO - Intern Med. 2026 Oct 29. doi: 10.2169/internalmedicine.8146-26. PMID- 42951581 OWN - NLM STAT- Publisher LR - 20261028 IS - 1349-7235 (Electronic) IS - 0918-2918 (Linking) DP - 2026 Oct 29 TI - Improving Hepatic Functional Reserve in Patients with Haemophilia and HIV/HCV-associated Cirrhosis: A Multicentre Prospective Study of Prolonged Foscenvivint Therapy. LID - 10.2169/internalmedicine.7831-26 [doi] AB - Objective　Patients with hemophilia who acquired HIV and HCV through contaminated clotting factor concentrates form a rare population with progressive cirrhosis in whom liver biopsy is contraindicated by bleeding risk, limiting therapeutic options. We evaluated the safety and preliminary efficacy of prolonged foscenvivint (OP-724), a selective modulator of CREB-binding protein/beta-catenin-dependent transcription, on the hepatic functional reserve in this population.Methods　In this multicenter, open-label, non-randomized, prospective study at three Japanese centers, consecutive adults with hemophilia and HIV/HCV-associated cirrhosis received intravenous foscenvivint 280 mg/m(2) over 3-4 hours, twice weekly for 24 weeks. The primary endpoint was the change in the albumin-bilirubin (ALBI) score from baseline to week 24. Secondary endpoints included the Child-Pugh score, liver stiffness (vibration-controlled transient elastography and magnetic resonance elastography), serum fibrosis markers, and safety with specific monitoring of bleeding events.Patients　Five adult males with hemophilia A (mean age 59.2 years) and HIV/HCV-associated cirrhosis, all with a sustained virologic response >/=48 months before enrolment.Results　Four patients completed the treatment; one discontinued treatment at week 22 due to non-treatment-related pyogenic arthritis. ALBI score improved in all evaluable patients, with mean changes from baseline of -0.420+/-0.130 at week 24 and -0.318+/-0.302 at week 28, four weeks after treatment completion. The serum albumin levels increased during treatment and remained above baseline at week 28. No bleeding- or treatment-related serious adverse events occurred.Conclusion　Prolonged foscenvivint was well tolerated and it was associated with an improved hepatic functional reserve during treatment in this biopsy-infeasible population. These findings are hypothesis-generating and suggest that noninvasive functional endpoints may be a feasible evaluation strategy, which requires confirmation in controlled studies. FAU - Kimura, Kiminori AU - Kimura K AD - Department of Hepatology, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, Japan. FAU - Ikoma, Akemi AU - Ikoma A AD - Department of Clinical Research and Trials Center, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, Japan. FAU - Okamoto, Noriyo AU - Okamoto N AD - Department of Hepatology, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, Japan. FAU - Endo, Tomoyuki AU - Endo T AD - Department of Hematology, Hokkaido University Hospital, Japan. FAU - Sakamori, Ryotaro AU - Sakamori R AD - Department of Gastroenterology and Hepatology, National Hospital Organization Osaka National Hospital, Japan. FAU - Yotsumoto, Mihoko AU - Yotsumoto M AD - Department of Laboratory Medicine, Tokyo Medical University, Japan. FAU - Imamura, Junji AU - Imamura J AD - HIV/AIDS Comprehensive Medical Center, National Hospital Organization Sendai Medical Center, Japan. FAU - Kimura, Masamichi AU - Kimura M AD - Department of Hepatology, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, Japan. FAU - Imamura, Jun AU - Imamura J AD - Department of Hepatology, Tokyo Metropolitan Cancer and Infectious Diseases Center, Komagome Hospital, Japan. FAU - Tsukada, Kunihisa AU - Tsukada K AD - Department of Infectious Diseases and Infection Control, Saitama Medical Center, Saitama Medical University, Japan. FAU - Ishikawa, Tetsuya AU - Ishikawa T AD - Department of Integrated Health Sciences, Nagoya University Graduate School of Medicine, Japan. FAU - Watanabe, Tsunamasa AU - Watanabe T AD - Division of Gastroenterology and Hepatology, St. Marianna University School of Medicine, Japan. FAU - Yotsuyanagi, Hiroshi AU - Yotsuyanagi H AD - Division of Infectious Diseases, Advanced Clinical Research Center, Institute of Medical Science, University of Tokyo, Japan. FAU - Gatanaga, Hiroyuki AU - Gatanaga H AD - AIDS Clinical Center, National Center for Global Health and Medicine, Japan Institute for Health Security, Japan. LA - eng PT - Journal Article DEP - 20261029 PL - Japan TA - Intern Med JT - Internal medicine (Tokyo, Japan) JID - 9204241 SB - IM OTO - NOTNLM OT - ALBI score OT - HIV/HCV coinfection OT - Hemophilia OT - Hepatic functional reserve OT - Liver cirrhosis EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 22:02 PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:02 [entrez] AID - 10.2169/internalmedicine.7831-26 [doi] PST - aheadofprint SO - Intern Med. 2026 Oct 29. doi: 10.2169/internalmedicine.7831-26. PMID- 42951580 OWN - NLM STAT- Publisher LR - 20261028 IS - 1349-7235 (Electronic) IS - 0918-2918 (Linking) DP - 2026 Oct 29 TI - A Case of Recurrent COVID-19 Pneumonia Following Rituximab Therapy: Treatment Informed by a Serial Transbronchial Lung Cryobiopsy, BALF SARS-CoV-2 PCR, and the Clinical Course. LID - 10.2169/internalmedicine.7725-26 [doi] AB - Coronavirus disease 2019 (COVID-19) presents with diagnostic and therapeutic challenges in immunocompromised patients, particularly those receiving anti-CD20 antibody therapy. This report describes a patient with recurrent pneumonia following COVID-19 after receiving anti-CD20 antibody therapy. Two transbronchial lung cryobiopsies documented active lung injury in two clinically distinct episodes. Based on an integrated assessment of the histopathological findings, bronchoalveolar lavage fluid SARS-CoV-2 PCR results, and the clinical course, antiviral and corticosteroid therapies were administered, followed by an improvement in the clinical course. This case suggests that cryobiopsy-based histopathology, integrated with the virological findings and clinical course, may provide complementary information for treatment selection in this patient population. FAU - Hayashi, Jun AU - Hayashi J AD - Department of Pulmonology, Kameda Medical Center, Japan. FAU - Nagai, Tatsuya AU - Nagai T AD - Department of Pulmonology, Kameda Medical Center, Japan. FAU - Seki, Kurumi AU - Seki K AD - Department of Pathology, Kameda Medical Center, Japan. FAU - Toho, Masanori AU - Toho M AD - Department of Medicine, Division of Hematology/Oncology, Kameda Medical Center, Japan. FAU - Sato, Yuki AU - Sato Y AD - Department of Pulmonology, Kameda Medical Center, Japan. FAU - Kawakami, Hiroki AU - Kawakami H AD - Department of Pulmonology, Kameda Medical Center, Japan. FAU - Yamaji, Soichiro AU - Yamaji S AD - Department of Pulmonology, Kameda Medical Center, Japan. FAU - Ito, Hikaru AU - Ito H AD - Department of Pulmonology, Kameda Medical Center, Japan. FAU - Otsuki, Ayumu AU - Otsuki A AD - Department of Pulmonology, Kameda Medical Center, Japan. FAU - Ito, Hiroyuki AU - Ito H AD - Department of Pulmonology, Kameda Medical Center, Japan. FAU - Osawa, Ryosuke AU - Osawa R AD - Department of Infectious Diseases, Kameda Medical Center, Japan. FAU - Fukuoka, Junya AU - Fukuoka J AD - Department of Pathology, Kameda Medical Center, Japan. FAU - Matsue, Kosei AU - Matsue K AD - Department of Medicine, Division of Hematology/Oncology, Kameda Medical Center, Japan. FAU - Nakashima, Kei AU - Nakashima K AD - Department of Pulmonology, Kameda Medical Center, Japan. LA - eng PT - Journal Article DEP - 20261029 PL - Japan TA - Intern Med JT - Internal medicine (Tokyo, Japan) JID - 9204241 SB - IM OTO - NOTNLM OT - COVID-19 OT - acute lung injury OT - persistent infection OT - rituximab OT - transbronchial lung cryobiopsy EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 22:02 PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:02 [entrez] AID - 10.2169/internalmedicine.7725-26 [doi] PST - aheadofprint SO - Intern Med. 2026 Oct 29. doi: 10.2169/internalmedicine.7725-26. PMID- 42951579 OWN - NLM STAT- Publisher LR - 20261028 IS - 1349-7235 (Electronic) IS - 0918-2918 (Linking) DP - 2026 Oct 29 TI - Recurrent Ischemic Stroke after Carotid Artery Stenting for Thyroid Cartilage Induced CCA Stenosis: Was Stenting an Appropriate Treatment? LID - 10.2169/internalmedicine.7599-26 [doi] AB - Mechanical stimulation of the common carotid artery (CCA) by the hyoid bone or thyroid cartilage can cause common carotid artery (CCA) stenosis and cerebral infarction. A 71-year-old man presented with symptomatic CCA stenosis. Imaging revealed CCA plaque at the site of contact with the thyroid cartilage, and ultrasonography revealed dynamic compression during swallowing. Despite technically successful carotid artery stenting, infarctions can recur without typical stent-related complications. The plaque may be trapped by the stent under persistent external mechanical stress, resulting in intermittent protrusions. Additional stenting prevented the recurrence. This case highlights the importance of considering external mechanical factors when selecting a treatment strategy. FAU - Kobayashi, Yuya AU - Kobayashi Y AD - Department of Neurology, Ina Central Hospital, Japan. FAU - Yui, Ryotaro AU - Yui R AD - Department of Neurology, Ina Central Hospital, Japan. FAU - Yuzawa, Chinami AU - Yuzawa C AD - Department of Neurology, Ina Central Hospital, Japan. FAU - Kurashina, Minori AU - Kurashina M AD - Department of Neurology, Ina Central Hospital, Japan. FAU - Sekijima, Yoshiki AU - Sekijima Y AD - Department of Medicine (Neurology & Rheumatology), Shinshu University School of Medicine, Japan. LA - eng PT - Journal Article DEP - 20261029 PL - Japan TA - Intern Med JT - Internal medicine (Tokyo, Japan) JID - 9204241 SB - IM OTO - NOTNLM OT - Carotid artery stenting OT - Common carotid artery stenosis OT - Hyoid bone OT - Plaque protrusion OT - Recurrent cerebral infarction OT - Thyroid cartilage EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 22:02 PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:02 [entrez] AID - 10.2169/internalmedicine.7599-26 [doi] PST - aheadofprint SO - Intern Med. 2026 Oct 29. doi: 10.2169/internalmedicine.7599-26. PMID- 42951578 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1882-6482 (Electronic) IS - 0021-5082 (Linking) VI - 81 DP - 2026 TI - [Environmental burden of generative artificial intelligence from a planetary health perspective: A narrative review]. LID - 10.1265/jjh.26004 [doi] AB - Generative artificial intelligence (GenAI) is widely used in healthcare to support evidence-based practice, policymaking, and research activities. Hundreds of millions of people worldwide use GenAI for a wide range of purposes, and this number continues to grow. In recent years, the discipline of "planetary health" has become mainstream as an urgent global issue, as excess mortality and morbidity attributable to extreme climate events have been reported worldwide. Although GenAI is increasingly used as a tool to promote planetary health, the rapid expansion of its use can add a substantial burden to the global environment. In Japan, however, the environmental impact of GenAI is rarely discussed. In this narrative review, we summarized quantitative data on the environmental impacts of GenAI on the basis of academic literature and reports from various industries. Data centers supporting the day-to-day operation of GenAI and the manufacturers of the required hardware consume vast amounts of electricity and water and emit large quantities of greenhouse gases. Furthermore, the mining of rare metals and the disposal of hardware release harmful chemicals that may contaminate soil and water. Unlike several other countries and international organizations, Japan currently lacks specific legislation and guidelines addressing the environmental impact of GenAI. The environmental burden of GenAI is underestimated because the reporting is largely voluntary and limited data are disclosed. Legislation, guidelines, and reporting standards promoting the transparent disclosure of environmental impacts will facilitate more reliable assessments. It is also important for individual users to make informed choices, such as selecting energy-efficient models or AI systems optimized for specific tasks. FAU - Miyata, Jun AU - Miyata J AD - Department of Island and Community Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. FAU - Noguchi, Naomi AU - Noguchi N AD - School of Public Health, Faculty of Medicine and Health, University of Sydney, New South Wales, Australia. LA - jpn PT - English Abstract PT - Journal Article PT - Review PL - Japan TA - Nihon Eiseigaku Zasshi JT - Nihon eiseigaku zasshi. Japanese journal of hygiene JID - 0417457 RN - 0 (Greenhouse Gases) SB - IM MH - *Generative Artificial Intelligence MH - Humans MH - Japan MH - *Environment MH - Greenhouse Gases MH - *Global Health MH - *Artificial Intelligence OTO - NOTNLM OT - energy supply OT - environmental pollution OT - generative artificial intelligence OT - greenhouse gas OT - planetary health OT - water supply EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 22:02 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:02 [entrez] AID - 10.1265/jjh.26004 [doi] PST - ppublish SO - Nihon Eiseigaku Zasshi. 2026;81. doi: 10.1265/jjh.26004. PMID- 42951574 OWN - NLM STAT- Publisher LR - 20261028 IS - 0546-1766 (Print) IS - 0546-1766 (Linking) DP - 2026 Oct 29 TI - [Development of an ICT utilization checklist for community-workplace collaboration promotion programs in local government: Consensus through a two-round Delphi study]. LID - 10.11236/jph.26-046 [doi] AB - Objective　To develop a checklist for utilizing information and communication technology (ICT) in community-workplace collaboration programs implemented by local government, based on a consensus among municipal personnel responsible for these programs in organizations utilizing multiple ICT tools.Methods　Using data from a questionnaire and interview surveys conducted in 2022-2023, a checklist comprising seven domains was drafted: online meetings (eight items), e-mail newsletters (seven items), websites (eight items), live online seminars (eight items), on-demand online seminars (eight items), health-related applications (nine items), and common foundational items across ICT utilization (eight items). The expert panel was selected from a nationwide survey conducted in June 2024 among prefectures and public health centers with community-workplace collaboration councils. Personnel responsible for community-workplace collaboration programs were invited from 54 organizations in which five or more ICT tools were utilized. A two-round Delphi study was conducted using an initial checklist based on the findings of the questionnaire and interview surveys. Participants rated the importance of items on a five-point scale, reported their implementation status, and provided open-ended comments. Consensus was defined as an agreement rate >/=70%, a median importance score >/=4, and an interquartile range ＜1.5.Results　Responses were obtained from 39 of 54 organizations (72.2%) in the first round, and 35 of 39 (89.7%) in the second round. The items were revised after the first round. In the second round, 55 items had an agreement rate >/=70%, and 53 items had an agreement rate >/=80%. The final checklist comprised 55 items across the seven domains: online meetings (eight items), e-mail newsletters (seven items), websites (eight items), live online seminars (eight items), on-demand online seminars (seven items), health-related applications (nine items), and common foundational items across ICT utilization (eight items).Conclusion　Using the Delphi method, a checklist was developed that encompasses the preparation, organizational and operational systems, implementation strategies, and evaluation components necessary for ICT utilization in community-workplace collaboration programs. Organizations can assess their Internet infrastructure and organizational readiness using the common foundational items before selectively applying the domain-specific checklists based on the ICT tools being implemented. The checklist may also assist organizations that are already using ICT, to periodically evaluate its effectiveness. Despite achieving consensus for all checklist items, several items related to budget, operational systems and evaluation had low implementation rates, indicating a necessity for strategies to enhance effective ICT utilization. FAU - Ozaki, Itsuko AU - Ozaki I AD - Graduate School of Nursing, Nagoya City University. FAU - Watai, Izumi AU - Watai I AD - Faculty of Nursing, Hamamatsu University School of Medicine. FAU - Zako, Yuri AU - Zako Y AD - Graduate School of Nursing, Nagoya City University. FAU - Obie, Kazuko AU - Obie K AD - Faculty of Nursing, Sugiyama Jogakuen University. FAU - Miyata, Ruriko AU - Miyata R AD - Faculty of Nursing, Suzuka University of Medical Science. FAU - Takabe, Sayaka AU - Takabe S AD - Faculty of Nursing, Hamamatsu University School of Medicine. FAU - Tsuzuki, Chikage AU - Tsuzuki C AD - Graduate School of Nursing, Osaka Metropolitan University. FAU - Yokoyama, Tetsuji AU - Yokoyama T AD - Department of Health Promotion, National Institute of Public Health. FAU - Tsushita, Kazuyo AU - Tsushita K AD - Faculty of Nutrition Sciences, Japan Nutrition University. LA - jpn PT - English Abstract PT - Journal Article DEP - 20261029 PL - Japan TA - Nihon Koshu Eisei Zasshi JT - [Nihon koshu eisei zasshi] Japanese journal of public health JID - 19130150R SB - IM OTO - NOTNLM OT - Delphi method OT - ICT utilization OT - checklist OT - community-workplace collaboration promotion programs EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 22:02 PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 22:02 [entrez] AID - 10.11236/jph.26-046 [doi] PST - aheadofprint SO - Nihon Koshu Eisei Zasshi. 2026 Oct 29. doi: 10.11236/jph.26-046. PMID- 42951566 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1425 DP - 2026 Dec 1 TI - Eutectogel-based thin film microextraction coupled with UPLC-PDA for selective detection of diflufenican. PG - 346331 LID - S0003-2670(26)01281-X [pii] LID - 10.1016/j.aca.2026.346331 [doi] AB - BACKGROUND: Per- and polyfluoroalkyl substance (PFAS) pesticides remain an under-quantified source of human dietary exposure, largely due to their trace-level occurrence and the complexity of food matrices. These analytical challenges underscore the need for selective extraction approaches capable of minimizing matrix interferences. RESULTS: In this work, a new thin-film microextraction (TFME) device incorporating multifunctional eutectogels was developed for the selective extraction and preconcentration of the PFAS herbicide diflufenican from food samples prior to UPLC-PDA quantification, and its performance was systematically evaluated. High-throughput screening of selectivity-enhanced deep eutectic solvents (DESs) toward diflufenican was performed using machine-learning-enhanced quantum chemical methods. Simultaneously, the eutectogel-based thin film was fabricated via UV-initiated photopolymerization of the DES, monomer, and crosslinker, yielding supramolecular gels stabilized by non-covalent interactions. The established method demonstrated excellent analytical performance, with a wide linear range (0.5-500 mug/L), a low LOD (0.04 mug/L and 0.53 mug/kg), and satisfactory recoveries (97.2-112%). Compared with the established methods, the proposed method possessed higher precision while affording a lower limit of detection and reduced hazardous reagent consumption. Mechanistic exploration corroborated by molecular simulations and multi-spectroscopic characterizations revealed that the eutectogel effectively captures diflufenican through hydrogen-bond interaction, pi-pi transpose, and proton-transfer inhibition, while the original DES interaction network undergoes dynamic reorganization. SIGNIFICANCE: The demonstrated tunability of the developed eutectogel-based thin-film microextraction device positions it as a versatile platform for the selective preconcentration of fluorinated species in complex matrices, supporting advances in the monitoring of hazardous substances in food. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Zhang, Ruixue AU - Zhang R AD - School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing, 100048, China. FAU - Fan, Chen AU - Fan C AD - School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing, 100048, China. Electronic address: fanchen@btbu.edu.cn. FAU - Li, Jie AU - Li J AD - School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing, 100048, China. FAU - Duan, Ran AU - Duan R AD - School of Light Industry Science and Engineering, Beijing Technology and Business University, Beijing, 100048, China. LA - eng PT - Journal Article DEP - 20260927 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 RN - 0 (Gels) RN - 0 (Deep Eutectic Solvents) RN - 0 (Herbicides) SB - IM MH - Gels/chemistry MH - Chromatography, High Pressure Liquid MH - *Liquid Phase Microextraction/methods MH - Food Contamination/analysis MH - Deep Eutectic Solvents/chemistry MH - *Herbicides/analysis OTO - NOTNLM OT - Deep eutectic solvent OT - Diflufenican OT - Food safety OT - PFAS pesticide OT - Preconcentration COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 21:56 PHST- 2026/07/21 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S0003-2670(26)01281-X [pii] AID - 10.1016/j.aca.2026.346331 [doi] PST - ppublish SO - Anal Chim Acta. 2026 Dec 1;1425:346331. doi: 10.1016/j.aca.2026.346331. Epub 2026 Sep 27. PMID- 42951565 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1425 DP - 2026 Dec 1 TI - Direct and highly sensitive impedimetric detection of creatinine in artificial urine using photopolymerized boronic-functionalized imprinted polymers films. PG - 346330 LID - S0003-2670(26)01280-8 [pii] LID - 10.1016/j.aca.2026.346330 [doi] AB - Highly sensitive creatinine (Cre) detection in complex urinary matrices remains challenging because of nonspecific interference and limited molecular selectivity. Here, a boronic acid-functionalized molecularly imprinted polymer (MIP) electrode was developed for label-free electrochemical impedance spectroscopy (EIS) detection of Cre in undiluted artificial urine. A Cre-imprinted film containing 4-vinylbenzeneboronic acid (4-VBBA) was photopolymerized on a vinyl-functionalized Au electrode to enhance molecular recognition. The optimized MIP electrode exhibited a linear dynamic range of 5-250 nM, a sensitivity of 0.027 nM(-1), and a limit of detection (LOD) of 1.63 nM. Its sensitivity was 6.75-fold higher than that of the non-imprinted polymer (NIP). Incorporation of 4-VBBA further increased the sensitivity from 0.020 to 0.027 nM(-1) and improved the selectivity coefficients from 3.12 to 5.76 to 4.49-8.57. The Cre response remained well preserved in the presence of a 10-fold excess of individual interferents. The sensor also demonstrated high fabrication reproducibility (RSD, 2.15%), operational repeatability over ten regeneration cycles (RSD, 2.67%), and analytical recoveries of 98.69-101.55%. These results establish the 4-VBBA-functionalized MIP as a robust and highly selective recognition interface for sensitive Cre sensing in complex urine-like matrices. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Lee, Yujin AU - Lee Y AD - Department of Polymer Science & Engineering, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 41566, Republic of Korea. FAU - Lim, Seok Jin AU - Lim SJ AD - Department of Polymer Science & Engineering, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 41566, Republic of Korea. FAU - Lee, Seung Hoon AU - Lee SH AD - School of Semiconductor Convergence Engineering, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 41566, Republic of Korea. FAU - Park, Jong Pil AU - Park JP AD - Department of Food Science and Technology and GreenTech-based Food Safety Research Group, BK21 Four, Chung-Ang University, Anseong, 17546, Republic of Korea. Electronic address: jppark@cau.ac.kr. FAU - Park, Jinyoung AU - Park J AD - Department of Polymer Science & Engineering, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 41566, Republic of Korea; School of Semiconductor Convergence Engineering, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 41566, Republic of Korea. Electronic address: jinpark@knu.ac.kr. LA - eng PT - Journal Article DEP - 20260926 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 RN - 0 (Boronic Acids) RN - AYI8EX34EU (Creatinine) RN - 0 (Molecularly Imprinted Polymers) RN - 0 (Polymers) RN - 0 (4-vinylphenyl boronic acid) RN - 0 (Vinyl Compounds) SB - IM MH - *Boronic Acids/chemistry MH - *Dielectric Spectroscopy/methods MH - *Creatinine/urine MH - *Molecularly Imprinted Polymers/chemistry MH - *Molecular Imprinting MH - Limit of Detection MH - Electrodes MH - Polymerization MH - *Polymers/chemistry MH - Humans MH - Photochemical Processes MH - Vinyl Compounds OTO - NOTNLM OT - 4-Vinylbenzeneboronic acid OT - Creatinine OT - Electrochemical impedance spectroscopy OT - Molecularly imprinted polymer OT - Photopolymerization COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:56 PHST- 2026/07/21 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S0003-2670(26)01280-8 [pii] AID - 10.1016/j.aca.2026.346330 [doi] PST - ppublish SO - Anal Chim Acta. 2026 Dec 1;1425:346330. doi: 10.1016/j.aca.2026.346330. Epub 2026 Sep 26. PMID- 42951562 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1425 DP - 2026 Dec 1 TI - Measurement uncertainty evaluation from analytical validation data: Asymmetric coverage interval due to uncorrected significant bias. PG - 346327 LID - S0003-2670(26)01277-8 [pii] LID - 10.1016/j.aca.2026.346327 [doi] AB - BACKGROUND: Reliability in analytical measurements depends on appropriate measurement uncertainty estimation. In top-down approaches, precision and recovery data obtained during analytical method validation are commonly used for this purpose. Although significant systematic effects should preferably be corrected, correction is not always feasible, and uncorrected bias may instead be incorporated into the uncertainty estimate. However, treating directional bias as a symmetric uncertainty component may result in unnecessarily wide intervals. This study proposes an asymmetric approach (ASYM) in which bias shifts the uncertainty interval without increasing the random uncertainty component. RESULTS: A Python-based Monte Carlo algorithm was developed to evaluate coverage probability and interval width over recovery values from 40% to 120% and precision up to 30%. ASYM was compared with a conventional root-sum-of-squares symmetric approach (RSSu or SYM) and alternative uncertainty models (SUMU, SUMUmax, and Ue). Moreover, the models were additionally evaluated using real analytical validation datasets from pesticide residues by LC-MS/MS. When bias was small relative to precision, the approaches generally provided similar coverage. As bias increased, ASYM and SUMU generally maintained coverage closer to the nominal 95% level, whereas other models produced wider intervals and misstated coverage levels. Application to real analytical data supported these trends. A practical decision framework based on the bias-to-precision ratio was suggested. SIGNIFICANCE AND NOVELTY: This study systematically evaluates asymmetric uncertainty estimation for uncorrected bias, distinguishing directional bias from random uncertainty. ASYM avoids artificially inflated intervals, provides coverage closer to the nominal level, and supports uncertainty model selection using the bias-to-precision ratio. CI - Copyright (c) 2026 The Authors. Published by Elsevier B.V. All rights reserved. FAU - do Nascimento Maranho, Ricardo Luiz AU - do Nascimento Maranho RL AD - Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes, 580, Sao Paulo, SP, 05508-000, Brazil. FAU - Lourenco, Felipe Rebello AU - Lourenco FR AD - Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of Sao Paulo, Av. Prof. Lineu Prestes, 580, Sao Paulo, SP, 05508-000, Brazil. Electronic address: feliperl@usp.br. LA - eng PT - Journal Article DEP - 20260925 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 SB - IM OTO - NOTNLM OT - Asymmetry OT - Bias OT - Coverage probability OT - Measurement uncertainty OT - Monte Carlo simulation COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:56 PHST- 2026/06/15 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S0003-2670(26)01277-8 [pii] AID - 10.1016/j.aca.2026.346327 [doi] PST - ppublish SO - Anal Chim Acta. 2026 Dec 1;1425:346327. doi: 10.1016/j.aca.2026.346327. Epub 2026 Sep 25. PMID- 42951560 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1425 DP - 2026 Dec 1 TI - Lysosome-targeted fluorescent probe for real-time imaging of hypochlorite in rheumatoid arthritis and machine learning-assisted quantification in plant tissues. PG - 346324 LID - S0003-2670(26)01274-2 [pii] LID - 10.1016/j.aca.2026.346324 [doi] AB - BACKGROUND: Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease closely associated with excessive oxidative stress. Hypochlorite (ClO(-)) are key reactive oxidants in inflammatory microenvironments which contribute to lysosomal dysfunction and inflammatory cascade amplification. However, rapid and selective visualization of ClO(-) in complex biological systems remains challenging. In addition, residual ClO(-) in plant-derived foods raises safety concerns, while conventional analytical methods are unsuitable for direct in situ detection. To address this issue, we developed a lysosome-targeted fluorescent probe HB-Z, for sensitive ClO(-) detection in RA models and plant tissues. RESULTS: The designed probe HB-Z consists of a 1,8-naphthalimide-based fluorophore, p-aminophenyl ether recognition unit, and morpholine lysosomal-targeting group, enabling remarkable turn-on fluorescence response upon ClO(-) triggered cleavage. Spectroscopic studies demonstrated excellent selectivity, rapid response within seconds, high sensitivity, and good pH stability. HB-Z successfully imaged exogenous and endogenous ClO(-) in live RAW 264.7 macrophages with distinct lysosomal localization. In a lambda-carrageenan-induced acute arthritis mouse model, HB-Z enabled visualization of elevated joint ClO(-) levels and real-time monitoring of oxidative fluctuations during disease progression and antioxidant-mediated remission. Furthermore, HB-Z achieved concentration-dependent fluorescence imaging of exogenous ClO(-) residues in soybean sprouts. By integrating fluorescence features and soaking time, a stochastic gradient descent machine-learning classifier further enabled three-class discrimination of disinfection status with high accuracy. SIGNIFICANCE: This probe provides a versatile platform for dynamic ClO(-) imaging in inflammatory diseases and intelligent assessment of food disinfection quality, possessing great potential for exploring the mechanism of inflammatory diseases and realizing intelligent food safety detection. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Zhang, Zhipeng AU - Zhang Z AD - State Key Laboratory of Flexible Electronics (LoFE) & Institute of Advanced Materials (IAM), School of Flexible Electronics (Future Technologies), Nanjing Tech University (NanjingTech), Nanjing, 211816, China. FAU - Chen, Zhengxin AU - Chen Z AD - State Key Laboratory of Flexible Electronics (LoFE) & Institute of Advanced Materials (IAM), School of Flexible Electronics (Future Technologies), Nanjing Tech University (NanjingTech), Nanjing, 211816, China. FAU - Wu, Hanbin AU - Wu H AD - State Key Laboratory of Flexible Electronics (LoFE) & Institute of Advanced Materials (IAM), School of Flexible Electronics (Future Technologies), Nanjing Tech University (NanjingTech), Nanjing, 211816, China. Electronic address: 202361122067@njtech.edu.cn. FAU - Huang, Zhongxi AU - Huang Z AD - State Key Laboratory of Flexible Electronics (LoFE) & Institute of Advanced Materials (IAM), School of Flexible Electronics (Future Technologies), Nanjing Tech University (NanjingTech), Nanjing, 211816, China. FAU - Zhang, Long AU - Zhang L AD - State Key Laboratory of Flexible Electronics (LoFE) & Institute of Advanced Materials (IAM), School of Flexible Electronics (Future Technologies), Nanjing Tech University (NanjingTech), Nanjing, 211816, China. FAU - Wang, Yao AU - Wang Y AD - State Key Laboratory of Flexible Electronics (LoFE) & Institute of Advanced Materials (IAM), School of Flexible Electronics (Future Technologies), Nanjing Tech University (NanjingTech), Nanjing, 211816, China. FAU - Ma, Zihan AU - Ma Z AD - State Key Laboratory of Flexible Electronics (LoFE) & Institute of Advanced Materials (IAM), School of Flexible Electronics (Future Technologies), Nanjing Tech University (NanjingTech), Nanjing, 211816, China. FAU - Pu, Yangyang AU - Pu Y AD - State Key Laboratory of Flexible Electronics (LoFE) & Institute of Advanced Materials (IAM), School of Flexible Electronics (Future Technologies), Nanjing Tech University (NanjingTech), Nanjing, 211816, China. FAU - Yu, Changmin AU - Yu C AD - State Key Laboratory of Flexible Electronics (LoFE) & Institute of Advanced Materials (IAM), School of Flexible Electronics (Future Technologies), Nanjing Tech University (NanjingTech), Nanjing, 211816, China. Electronic address: iamcmyu@njtech.edu.cn. LA - eng PT - Journal Article DEP - 20260925 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 RN - 712K4CDC10 (Hypochlorous Acid) RN - 0 (Fluorescent Dyes) RN - 0 (Fluorescent Chemosensor Compounds) SB - IM MH - *Hypochlorous Acid/analysis/metabolism MH - Animals MH - *Fluorescent Dyes/chemistry/chemical synthesis MH - *Machine Learning MH - *Arthritis, Rheumatoid/diagnostic imaging/metabolism/chemically induced MH - *Lysosomes/metabolism/chemistry MH - Mice MH - RAW 264.7 Cells MH - Fluorescent Chemosensor Compounds MH - Optical Imaging OTO - NOTNLM OT - Fluorescent probe OT - Hypochlorite OT - Machine learning OT - Rheumatoid arthritis COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:56 PHST- 2026/06/25 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S0003-2670(26)01274-2 [pii] AID - 10.1016/j.aca.2026.346324 [doi] PST - ppublish SO - Anal Chim Acta. 2026 Dec 1;1425:346324. doi: 10.1016/j.aca.2026.346324. Epub 2026 Sep 25. PMID- 42951558 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1425 DP - 2026 Dec 1 TI - A comparison of PCA, ASCA, and vASCA for enhancing the interpretability of biological Raman spectroscopy. PG - 346321 LID - S0003-2670(26)01271-7 [pii] LID - 10.1016/j.aca.2026.346321 [doi] AB - BACKGROUND: Raman spectroscopy is often used to analyze complex biological systems, but interpreting the resulting spectra remains difficult because of overlapping vibrational bands and the limitations of standard multivariate approaches such as Principal Component Analysis. This work assesses whether ANOVA-Simultaneous Component Analysis and its variable-selection extension can address these interpretability issues. More specifically, the goal was to determine if this approach could isolate spectral features that reflect metabolic syndrome and its progression over time. RESULTS: Serum Raman spectra were collected from control rats and those with metabolic syndrome at four time points. The datasets were then analyzed comparatively using Principal Component Analysis, ANOVA-Simultaneous Component Analysis, and Variable-Selection ANOVA-Simultaneous Component Analysis, with attention not only to statistical separation but also to the interpretability of the resulting features in a biochemical context. Variable-Selection ANOVA-Simultaneous Component Analysis shows a narrower set of statistically relevant spectral regions. Among these, a Raman band in the 907-926 cm(-1) range appeared as a candidate signature associated with metabolic syndrome. Temporal variation was also evident, particularly in lipid- and protein-related bands between 8 and 52 weeks, although some overlap across groups persisted. Compared with the other methods, this approach yielded a clearer feature-based separation and, in most comparisons, stronger statistical contrasts. SIGNIFICANCE AND NOVELTY: The use of Variable-Selection ANOVA-Simultaneous Component Analysis makes the relationship between spectral features and the physiology of the disease clearer. Our approach paves the way to establish an interpretable tool to identify relevant spectral signatures in biological Raman spectra datasets. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Ramirez-Elias, Miguel G AU - Ramirez-Elias MG AD - Facultad de Ciencias, Universidad Autonoma de San Luis Potosi, Av. Chapultepec 1570, Privadas del Pedregal, San Luis Potosi, 78295, Mexico. Electronic address: miguel.ghebre@uaslp.mx. FAU - Vallejo-Espana, Daniel AU - Vallejo-Espana D AD - Research Centre for Information and Communication Technologies (CITIC-UGR), University of Granada, Granada, 18014, Spain; Instituto de Investigacion Biosanitaria ibs.GRANADA, Granada, Spain. FAU - Donjuan-Loredo, Guadalupe AU - Donjuan-Loredo G AD - Facultad de Medicina, Universidad Autonoma de San Luis Potosi, Av. Venustiano Carranza 2405, Lomas los Filtros, San Luis Potosi, 78210, Mexico. FAU - Camacho, Jose AU - Camacho J AD - Research Centre for Information and Communication Technologies (CITIC-UGR), University of Granada, Granada, 18014, Spain; Instituto de Investigacion Biosanitaria ibs.GRANADA, Granada, Spain. LA - eng PT - Comparative Study PT - Journal Article DEP - 20260923 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 SB - IM MH - *Spectrum Analysis, Raman/methods MH - Animals MH - *Principal Component Analysis MH - Rats MH - *Metabolic Syndrome/blood MH - Analysis of Variance OTO - NOTNLM OT - ANOVA-simultaneous component analysis OT - Chemometrics OT - Metabolic syndrome OT - Raman spectroscopy OT - Variable selection COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Miguel G. Ramirez-Elias reports financial support was provided by Iberoamerican University Postgraduate Association. Miguel G. Ramirez-Elias reports financial support was provided by Government of Andalusia Ministry of Economic Transformation Industry Knowledge and Universities. Jose Camacho reports financial support was provided by State Agency of Research. Jose Camacho reports financial support was provided by European Regional Development Fund. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:56 PHST- 2026/07/16 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S0003-2670(26)01271-7 [pii] AID - 10.1016/j.aca.2026.346321 [doi] PST - ppublish SO - Anal Chim Acta. 2026 Dec 1;1425:346321. doi: 10.1016/j.aca.2026.346321. Epub 2026 Sep 23. PMID- 42951552 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1425 DP - 2026 Dec 1 TI - HOF-101/AgInS(2) photogate-engineered organic photoelectrochemical transistor for amplified miRNA-21 biosensing. PG - 346315 LID - S0003-2670(26)01265-1 [pii] LID - 10.1016/j.aca.2026.346315 [doi] AB - BACKGROUND: Nucleic acid detection requires sensitive and controllable signal transduction strategies for reliable bioanalysis. Organic photoelectrochemical transistor (OPECT) provides a promising platform for transistor-level signal amplification, while its analytical performance is strongly dependent on photogate design and interfacial signal transduction. RESULTS: Herein, we report a photogate-engineered OPECT for transistor-amplified biosensing of miRNA-21, enabled by a HOF-101/AgInS(2) heterojunction. The integration of AgInS(2) quantum dots with a photosensitive hydrogen-bonded organic framework (HOF-101) forms an efficient photogate, promoting photoinduced charge separation and enabling light-driven modulation of the interfacial gate potential. Upon target recognition, catalytic hairpin assembly (CHA) triggers rolling circle amplification (RCA), producing G-rich DNA strands that, in the presence of hemin, generate peroxidase-mimetic catalytic centers. These catalytic units then mediate enzymatic precipitation at the photogate interface, leading to the accumulation of insulating deposits. The resulting interfacial deposition modulates the photogating response and effective gate voltage, thereby transducing molecular recognition events into amplified transistor current responses. This molecular-to-transistor signal transduction pathway enables highly sensitive and selective measurement of miRNA-21. The present design provides a strategy for integrating photoresponsive interface engineering with transistor-level amplification for advanced bioanalysis. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Shi, Xiao-Mei AU - Shi XM AD - Institute of Biomedical Engineering and Health Sciences, School of Medical and Health Engineering, Changzhou University, Changzhou, 213164, China. FAU - Li, Pengyin AU - Li P AD - Institute of Biomedical Engineering and Health Sciences, School of Medical and Health Engineering, Changzhou University, Changzhou, 213164, China. FAU - Che, Bo AU - Che B AD - Institute of Biomedical Engineering and Health Sciences, School of Medical and Health Engineering, Changzhou University, Changzhou, 213164, China. FAU - Deng, Linhong AU - Deng L AD - Institute of Biomedical Engineering and Health Sciences, School of Medical and Health Engineering, Changzhou University, Changzhou, 213164, China. Electronic address: dlh@cczu.edu.cn. FAU - Fan, Gao-Chao AU - Fan GC AD - College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China. Electronic address: gcfan@qust.edu.cn. LA - eng PT - Journal Article DEP - 20260922 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 RN - 0 (MicroRNAs) RN - 0 (MIRN21 microRNA, human) RN - 045A6V3VFX (Indium) RN - 3M4G523W1G (Silver) SB - IM MH - *MicroRNAs/analysis MH - *Biosensing Techniques/methods/instrumentation MH - *Electrochemical Techniques/instrumentation/methods MH - *Transistors, Electronic MH - *Indium/chemistry MH - Photochemical Processes MH - Humans MH - Silver/chemistry MH - Quantum Dots/chemistry OTO - NOTNLM OT - Cascade amplification OT - HOF-101/AgInS(2) heterojunction OT - Organic photoelectrochemical transistor OT - Photogate engineering OT - miRNA-21 COIS- Declaration of competing interest We hereby declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work. Furthermore, we affirm that there are no professional or personal interests of any kind in any products, services, or companies that could be construed as influencing the position presented in, or the review of, the manuscript entitled. EDAT- 2026/10/28 23:27 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 21:56 PHST- 2026/08/15 00:00 [received] PHST- 2026/09/13 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S0003-2670(26)01265-1 [pii] AID - 10.1016/j.aca.2026.346315 [doi] PST - ppublish SO - Anal Chim Acta. 2026 Dec 1;1425:346315. doi: 10.1016/j.aca.2026.346315. Epub 2026 Sep 22. PMID- 42951550 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1425 DP - 2026 Dec 1 TI - l-Histidine functionalized MXene quantum dots as novel fluorescent sensors for wide range detection of methyl orange. PG - 346313 LID - S0003-2670(26)01263-8 [pii] LID - 10.1016/j.aca.2026.346313 [doi] AB - The wide-range and highly selective detection of toxic dye molecules remains an important challenge in environmental analysis. As an organic synthetic dye, methyl orange (MO) can pose considerable health risks even at low concentrations. Although diverse analytical approaches have been established for the detection of MO, the simultaneous achievement of a broad detection range and high selectivity remains challenging. Herein, nitrogen-functionalized MXene quantum dots (N-MQDs) were fabricated and employed for the first time as fluorescent probes for MO detection. N-MQDs were obtained using 2D MXene Ti(3)C(2) nanosheets and l-histidine as raw chemicals via the hydrothermal method. Notably, l-histidine, a non-toxic dopant, was utilized for the first time in the preparation of N-MQDs. Furthermore, the introduction of l-histidine contributed to the passivation of surface defect sites in N-MQDs, resulting in bright fluorescence emission. N-MQDs exhibited a detection limit of 0.02 muM for MO and a detection range of 0.05-450 muM, which is wider than those of most reported methods for MO detection. N-MQDs were applied to the detection of MO in real water samples, and satisfactory spike recovery values were obtained. Mechanistic investigation of the response toward MO demonstrated that the fluorescence quenching of the N-MQDs by MO was attributed to Forster resonance energy transfer. The results obtained in this work demonstrate that N-MQDs are promising fluorescence probes for MO detection and show great promise in environmental monitoring applications. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Zhao, Libing AU - Zhao L AD - Key Laboratory of Special Functional Materials for Ecological Environment and Information (Hebei University of Technology), Ministry of Education, Tianjin, 300130, People's Republic of China; School of Materials Science and Engineering, Hebei University of Technology, Tianjin, 300130, People's Republic of China. FAU - Ji, Shijun AU - Ji S AD - Key Laboratory of Special Functional Materials for Ecological Environment and Information (Hebei University of Technology), Ministry of Education, Tianjin, 300130, People's Republic of China; School of Materials Science and Engineering, Hebei University of Technology, Tianjin, 300130, People's Republic of China. FAU - Xuan, Qishun AU - Xuan Q AD - Key Laboratory of Special Functional Materials for Ecological Environment and Information (Hebei University of Technology), Ministry of Education, Tianjin, 300130, People's Republic of China; School of Materials Science and Engineering, Hebei University of Technology, Tianjin, 300130, People's Republic of China. FAU - Tang, Jiayi AU - Tang J AD - Key Laboratory of Special Functional Materials for Ecological Environment and Information (Hebei University of Technology), Ministry of Education, Tianjin, 300130, People's Republic of China; School of Materials Science and Engineering, Hebei University of Technology, Tianjin, 300130, People's Republic of China. FAU - Sun, Jian-Feng AU - Sun JF AD - Key Laboratory of Special Functional Materials for Ecological Environment and Information (Hebei University of Technology), Ministry of Education, Tianjin, 300130, People's Republic of China; School of Materials Science and Engineering, Hebei University of Technology, Tianjin, 300130, People's Republic of China. Electronic address: sunjianfeng10@hebut.edu.cn. LA - eng PT - Journal Article DEP - 20260922 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 SB - IM OTO - NOTNLM OT - Dye molecules OT - Fluorescence OT - Fluorescent detection OT - Forster resonance energy transfer OT - MXene quantum dots COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:56 PHST- 2026/06/22 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S0003-2670(26)01263-8 [pii] AID - 10.1016/j.aca.2026.346313 [doi] PST - ppublish SO - Anal Chim Acta. 2026 Dec 1;1425:346313. doi: 10.1016/j.aca.2026.346313. Epub 2026 Sep 22. PMID- 42951548 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1425 DP - 2026 Dec 1 TI - Fluorescent labeling of lysosomal alpha-L-fucosidase using an activity-based near-infrared (NIR) fluorogenic probe. PG - 346311 LID - S0003-2670(26)01261-4 [pii] LID - 10.1016/j.aca.2026.346311 [doi] AB - BACKGROUND: Lysosomal alpha-L-fucosidase catalyzes the degradation of fucosylated glycoconjugates and is implicated in multiple pathological events. Although conventional fluorogenic substrates have been utilized to detect the glycosidase in cells, they are inappropriate for long-term imaging owing to the diffusion of the fluorophores released upon enzymatic cleavage. On the other hand, existing activity-based probes are useful for capturing the target enzyme, but they cannot be readily used for live-cell fluorescence imaging. RESULTS: We developed alphaFuc-Bn-NIR(CM)-AY, an activity-based near-infrared (NIR) fluorogenic probe for selective fluorescence imaging and covalent labeling of active alpha-L-fucosidase. The probe comprised (1) an alpha-L-fucose recognition unit linked to a hemicyanine-derived NIR fluorophore through a self-immolative scaffold, (2) a carbamate-based reactive moiety appended to the fluorophore, and (3) a terminal alkyne bioorthogonal handle. The probe functioned as a mechanism-based, irreversible inhibitor that covalently labeled and inactivated active alpha-L-fucosidase upon fluorescence activation. In addition, alphaFuc-Bn-NIR(CM)-AY showed high selectivity toward alpha-L-fucosidase and enabled robust fluorescent labeling of the enzyme. Moreover, the probe allowed detection of the enzyme at concentrations as low as 20 nM, and the labeling was markedly reduced by an alpha-L-fucosidase inhibitor. In live cells, enzyme-mediated activation produced persistent lysosome-associated fluorescence with minimal cytotoxicity. The terminal alkyne further facilitated click chemistry-mediated biotinylation and affinity enrichment of labeled lysosomal alpha-L-fucosidase from treated cells. SIGNIFICANCE: alphaFuc-Bn-NIR(CM)-AY integrates fluorescence activation, covalent enzyme labeling, and affinity-based enrichment within a single NIR platform, overcoming the diffusion-associated limitation of conventional fluorogenic substrates and the imaging limitation of existing activity-based probes. It provides a useful tool for prolonged imaging and covalent capture of active lysosomal alpha-L-fucosidase. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Kim, Yujun AU - Kim Y AD - Department of Chemistry, Yonsei University, Seoul, 03722, Republic of Korea. FAU - Lee, Chang-Hee AU - Lee CH AD - Department of Chemistry, Yonsei University, Seoul, 03722, Republic of Korea. FAU - Shin, Injae AU - Shin I AD - Department of Chemistry, Yonsei University, Seoul, 03722, Republic of Korea. Electronic address: injae@yonsei.ac.kr. LA - eng PT - Journal Article DEP - 20260921 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 RN - EC 3.2.1.51 (alpha-L-Fucosidase) RN - 0 (Fluorescent Dyes) RN - 0 (Fluorescent Chemosensor Compounds) SB - IM MH - *alpha-L-Fucosidase/metabolism/analysis/antagonists & inhibitors/chemistry MH - *Fluorescent Dyes/chemistry/chemical synthesis MH - Humans MH - *Lysosomes/enzymology MH - Fluorescent Chemosensor Compounds MH - Animals MH - Optical Imaging MH - Infrared Rays OTO - NOTNLM OT - Activity-based probe OT - Fluorogenic probe OT - Lysosome targeting OT - Near-infrared imaging OT - alpha-L-fucosidase COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:56 PHST- 2026/06/30 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S0003-2670(26)01261-4 [pii] AID - 10.1016/j.aca.2026.346311 [doi] PST - ppublish SO - Anal Chim Acta. 2026 Dec 1;1425:346311. doi: 10.1016/j.aca.2026.346311. Epub 2026 Sep 21. PMID- 42951545 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1425 DP - 2026 Dec 1 TI - Magnetic solid-phase extraction coupled with fluorescent lateral flow immunoassay for simultaneous on-site detection of methamphetamine, morphine, and ketamine in wastewater. PG - 346308 LID - S0003-2670(26)01258-4 [pii] LID - 10.1016/j.aca.2026.346308 [doi] AB - BACKGROUND: Wastewater-based epidemiology provides objective, non-invasive data on community drug use, but conventional ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis is laboratory-based and time-consuming. Although fluorescent lateral flow immunoassay (LFIA) offers a simple, rapid, and low-cost alternative for on-site screening, its direct application to wastewater is limited by low analyte concentrations and complex sample matrices. RESULTS: We developed a magnetic solid-phase extraction (MSPE)-LFIA platform based on polydopamine-coated magnetic nanoparticle adsorbents for simultaneous detection of methamphetamine, morphine, and ketamine in wastewater. By investigating the organic solvent compatibility of commercial LFIA strips and using an acidic eluent with low methanol content, the nitrogen blowdown reconstitution step was avoided. Under optimized conditions, the proposed method achieved limits of detection of 13.6, 14.4, and 50.8 ng L(-1), offering 66-, 61-, and 45-fold higher sensitivity than direct LFIA, with recoveries of 86.8-115.7% and RSDs below 12.3%. The entire procedure was completed within 15 min without bulky instrumentation. The method was applied to real wastewater samples, and the results exhibited good consistency with UPLC-MS/MS and a lower false-negative rate (16.7%) compared with direct LFIA (100%). SIGNIFICANCE: This work provides the first MSPE-LFIA platform for simultaneous on-site screening of methamphetamine, morphine, and ketamine in wastewater. This platform enabled direct eluate loading and eliminated the nitrogen blowdown step, thereby simplifying the workflow. Given its simplicity and low cost, it is expected to be applicable to multiplex detection of common illicit drugs or other hazardous organic pollutants in wastewater. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Hu, Cong AU - Hu C AD - Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences & Center for Medical Research and Innovation, Shanghai Pudong Hospital, Pudong Medical Center, Fudan University, Shanghai, 201203, China; Department of Forensic Toxicology, Academy of Forensic Science, Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Science Platform, Key Laboratory of Forensic Sciences, Ministry of Justice, Shanghai, 200063, China. FAU - Hou, Jingxin AU - Hou J AD - Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences & Center for Medical Research and Innovation, Shanghai Pudong Hospital, Pudong Medical Center, Fudan University, Shanghai, 201203, China. FAU - Zhu, Ruiqin AU - Zhu R AD - Department of Forensic Toxicology, Academy of Forensic Science, Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Science Platform, Key Laboratory of Forensic Sciences, Ministry of Justice, Shanghai, 200063, China. FAU - Feng, Jianan AU - Feng J AD - School of Pharmacy, Shanghai University of Medicine and Health Sciences, Shanghai, 201318, China. FAU - Yuan, Shuai AU - Yuan S AD - Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences & Center for Medical Research and Innovation, Shanghai Pudong Hospital, Pudong Medical Center, Fudan University, Shanghai, 201203, China; Department of Forensic Toxicology, Academy of Forensic Science, Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Science Platform, Key Laboratory of Forensic Sciences, Ministry of Justice, Shanghai, 200063, China. FAU - Li, Hanyin AU - Li H AD - Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences & Center for Medical Research and Innovation, Shanghai Pudong Hospital, Pudong Medical Center, Fudan University, Shanghai, 201203, China. FAU - Xiang, Ping AU - Xiang P AD - Department of Forensic Toxicology, Academy of Forensic Science, Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Science Platform, Key Laboratory of Forensic Sciences, Ministry of Justice, Shanghai, 200063, China. Electronic address: xiangping2630@163.com. FAU - Li, Yan AU - Li Y AD - Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences & Center for Medical Research and Innovation, Shanghai Pudong Hospital, Pudong Medical Center, Fudan University, Shanghai, 201203, China. Electronic address: yanli@fudan.edu.cn. LA - eng PT - Journal Article DEP - 20260922 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 RN - 76I7G6D29C (Morphine) RN - 44RAL3456C (Methamphetamine) RN - 0 (Wastewater) RN - 690G0D6V8H (Ketamine) RN - 0 (Water Pollutants, Chemical) RN - 0 (Magnetite Nanoparticles) RN - 0 (Indoles) RN - 0 (polydopamine) RN - 0 (Polymers) SB - IM MH - *Solid Phase Extraction/methods MH - *Morphine/analysis/isolation & purification MH - *Methamphetamine/analysis MH - *Wastewater/analysis/chemistry MH - Immunoassay/methods MH - *Ketamine/analysis/isolation & purification MH - *Water Pollutants, Chemical/analysis/isolation & purification MH - Magnetite Nanoparticles/chemistry MH - Indoles/chemistry MH - Limit of Detection MH - Polymers OTO - NOTNLM OT - Drugs of abuse OT - Immunochromatographic assay OT - Magnetic solid-phase extraction OT - Rapid screening OT - Wastewater COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 21:56 PHST- 2026/08/24 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S0003-2670(26)01258-4 [pii] AID - 10.1016/j.aca.2026.346308 [doi] PST - ppublish SO - Anal Chim Acta. 2026 Dec 1;1425:346308. doi: 10.1016/j.aca.2026.346308. Epub 2026 Sep 22. PMID- 42951543 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1425 DP - 2026 Dec 1 TI - Complementary molecular windows revealed by direct NanoESI and SPE-ESI FT-ICR-MS for dissolved organic matter characterization. PG - 346306 LID - S0003-2670(26)01256-0 [pii] LID - 10.1016/j.aca.2026.346306 [doi] AB - Nanoelectrospray ionization (nanoESI) coupled with Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) has emerged as a powerful approach for the direct molecular characterization of dissolved organic matter (DOM) without solid-phase extraction (SPE). However, the differences in molecular coverage and compositional selectivity between direct nanoESI and conventional SPE-ESI for DOM analysis remain unclear. In this study, two standard reference materials, Suwannee River natural organic matter (SRNOM) and Elliott soil fulvic acid (ESFA), were used to systematically compare two ionization sources (nanoESI and ESI) and two sample introduction modes (direct injection and SPE pretreatment). Compared with conventional ESI, nanoESI increased total ion intensity by 4.5-fold and 3.9-fold for SRNOM and ESFA, respectively, and expanded assigned molecular formulas by 1.5-1.7 times. ESI yielded a greater number of aromatic and highly unsaturated compounds, whereas nanoESI enabled broader molecular coverage, including aliphatic compounds, oxygen-rich aromatics, and heteroatom-containing species. SPE pretreatment enriched lignins and condensed aromatics in both samples, while direct nanoESI retained more lipid- and peptide-like compounds in SRNOM and abundant aminosugar- and carbohydrate-like compounds in ESFA. Combing the ionization and pretreatment effects, SPE-ESI favors the detection of aromatic fractions, whereas direct nanoESI better captures aliphatic- and tannin-like components. The two methods yield complementary information and enabled more comprehensive and unbiased molecular profiling of environmental DOM. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Zhao, Yanping AU - Zhao Y AD - Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology, Institute of Analysis, Guangdong Academy of Sciences (China National Analytical Center, Guangzhou), Guangzhou, 510070, China. FAU - Han, Yue AU - Han Y AD - Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology, Institute of Analysis, Guangdong Academy of Sciences (China National Analytical Center, Guangzhou), Guangzhou, 510070, China. FAU - Chen, Chao AU - Chen C AD - Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology, Institute of Analysis, Guangdong Academy of Sciences (China National Analytical Center, Guangzhou), Guangzhou, 510070, China. FAU - Han, Huixia AU - Han H AD - Institute of Surface-Earth System Science, Tianjin University, Tianjin, 300072, China. FAU - Yang, Yunyun AU - Yang Y AD - Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology, Institute of Analysis, Guangdong Academy of Sciences (China National Analytical Center, Guangzhou), Guangzhou, 510070, China. FAU - Song, Yumei AU - Song Y AD - Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology, Institute of Analysis, Guangdong Academy of Sciences (China National Analytical Center, Guangzhou), Guangzhou, 510070, China. FAU - Chen, Zhiliang AU - Chen Z AD - South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou, 510006, China. FAU - Fu, Pingqing AU - Fu P AD - Institute of Surface-Earth System Science, Tianjin University, Tianjin, 300072, China. FAU - Guo, Pengran AU - Guo P AD - Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology, Institute of Analysis, Guangdong Academy of Sciences (China National Analytical Center, Guangzhou), Guangzhou, 510070, China. Electronic address: prguo@fenxi.com.cn. LA - eng PT - Journal Article DEP - 20260921 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 SB - IM OTO - NOTNLM OT - Dissolved organic matter OT - Electrospray ionization OT - Fourier transform ion cyclotron resonance mass spectrometry OT - Ionization selectivity OT - Nanoelectrospray ionization OT - Solid-phase extraction COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:56 PHST- 2026/06/21 00:00 [received] PHST- 2026/09/09 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S0003-2670(26)01256-0 [pii] AID - 10.1016/j.aca.2026.346306 [doi] PST - ppublish SO - Anal Chim Acta. 2026 Dec 1;1425:346306. doi: 10.1016/j.aca.2026.346306. Epub 2026 Sep 21. PMID- 42951539 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1425 DP - 2026 Dec 1 TI - Synergistic dual-signal amplification aptasensor and AuNPs@TtTp COF-based SERS platform for ultrasensitive detection of SARS-CoV-2 receptor-binding domain. PG - 346302 LID - S0003-2670(26)01252-3 [pii] LID - 10.1016/j.aca.2026.346302 [doi] AB - BACKGROUND: Early and accurate detection of the virus SARS-CoV-2, responsible for COVID-19 pandemic, is essential for controlling the disease spread. However, existing diagnostic platforms are constrained by cost, turnaround time, or limited sensitivity. Surface-enhanced Raman scattering (SERS) technique offers an attractive alternative, though conventional SERS substrates suffer from limited reproducibility and weak, non-specific analyte binding. RESULTS: This study presents developing a highly sensitive and selective signal-off SERS aptasensor for detecting SARS-CoV-2 by combining a gold-nanoparticle-embedded covalent organic framework (AuNPs@TtTp COF) substrate with Au@4ATP@Ag@Au core-shell SERS tags functionalized with the virus RBD (receptor binding domain)-specific DNA aptamer. Target-induced disassembly of the tag-aptamer-COF hotspot architecture produces a concentration-dependent decrease in SERS intensity, giving a detection limit of 1.3 x 10(-17) g/mL over a dynamic range of 1 x 10(-16) - 1 x 10(-10) g/mL, with RSD values of 6.65 - 7.61% across replicate spots and substrates. Molecular dynamics simulations of three candidate aptamer-RBD complexes identifies the TZ1 model as most stable (binding free energy -57.99 kcal/mol). The aptasensor detects RBD in diluted human saliva, with results closely matching those of a commercial antigen test-kit. SIGNIFICANCE: By embedding gold nanoparticles within a triazine-linked COF and pairing them with dual-shell Au@4ATP@Ag@Au SERS tags, this platform achieves reproducible, ultrasensitive detection of the SARS-CoV-2 RBD, several orders of magnitude below other alternatives and outperforming previously reported SERS-based sensors for the same target. Because the design relies only on swapping the aptamer sequence to redirect target recognition, this strategy offers a readily adaptable template for rapid, low-cost diagnostics against other viral pathogens, and its performance in real saliva samples points toward a practical route from bench-scale detection to point-of-care screening. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Pal, Anabadya AU - Pal A AD - Department of Biochemistry and Biophysics, University of Kalyani, Kalyani, West Bengal, 741235, India. FAU - Chaudhuri, Ankur AU - Chaudhuri A AD - Structural Biology and Bioinformatics Division, CSIR-Indian Institute of Chemical Biology, Kolkata, West Bengal, 700091, India. FAU - Azaharuddin, Md AU - Azaharuddin M AD - Department of Biochemistry and Biophysics, University of Kalyani, Kalyani, West Bengal, 741235, India. FAU - Roy, Amitesh AU - Roy A AD - Department of Chemistry, University of Kalyani, Kalyani, West Bengal, 741235, India. FAU - Chakrabarty, Soumajit AU - Chakrabarty S AD - Department of Biochemistry and Biophysics, University of Kalyani, Kalyani, West Bengal, 741235, India. FAU - Nandi, Susmita AU - Nandi S AD - Department of Biochemistry and Biophysics, University of Kalyani, Kalyani, West Bengal, 741235, India. FAU - Chakrabarti, Saikat AU - Chakrabarti S AD - Structural Biology and Bioinformatics Division, CSIR-Indian Institute of Chemical Biology, Kolkata, West Bengal, 700091, India. FAU - Basu, Tarakdas AU - Basu T AD - Department of Biochemistry and Biophysics, University of Kalyani, Kalyani, West Bengal, 741235, India. Electronic address: tarakdb@yahoo.com. LA - eng PT - Journal Article DEP - 20260923 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 RN - 7440-57-5 (Gold) RN - 0 (Aptamers, Nucleotide) SB - IM MH - *Spectrum Analysis, Raman/methods MH - Gold/chemistry MH - *Aptamers, Nucleotide/chemistry MH - *Metal Nanoparticles/chemistry MH - *SARS-CoV-2/isolation & purification/metabolism/chemistry MH - Humans MH - Limit of Detection MH - *COVID-19/diagnosis/virology MH - *Biosensing Techniques/methods MH - Saliva/virology MH - Rapid Diagnostic Tests OTO - NOTNLM OT - Aptasensor OT - Covalent organic framework (COF) OT - RBD OT - SARS-CoV-2 OT - Surface-enhanced Raman spectroscopy (SERS) COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:56 PHST- 2026/07/08 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S0003-2670(26)01252-3 [pii] AID - 10.1016/j.aca.2026.346302 [doi] PST - ppublish SO - Anal Chim Acta. 2026 Dec 1;1425:346302. doi: 10.1016/j.aca.2026.346302. Epub 2026 Sep 23. PMID- 42951534 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1425 DP - 2026 Dec 1 TI - Electrochemical profiling of urinary extracellular vesicle proteomes to reveal differential hypoxic adaptation. PG - 346296 LID - S0003-2670(26)01246-8 [pii] LID - 10.1016/j.aca.2026.346296 [doi] AB - Hypobaric hypoxia induces physiological stress, yet early molecular alterations preceding functional impairment are not adequately captured by conventional physiologic analysis. Extracellular vesicles (EVs), encapsulated proteins and RNAs reflective of cellular state, represent a compelling source of non-invasive biomarkers for interrogating hypoxia-induced molecular adaptation at the proteome and post-transcriptional levels. Here, we present an electrochemical-proteomic framework for quantitative profiling of urinary EV (uEV) cargo during hypobaric hypoxic exposure. Two analog astronauts were monitored during a 10-day high-altitude expedition (14,860 ft). Pre-, mid-, and post-exposure physiological assessments using pulse oximetry and spirometry were complemented by molecular analysis of uEVs. EVs were captured directly on a screen-printed gold electrode-based immunosensor, followed by electric-field lysis for offering on-chip access to intravesicular cargo. EV enrichment and hypoxic stress responses were validated by Western blotting. Non-faradaic electrochemical impedance spectroscopy enables quantification of EV-associated proteins, including epithelial secretory proteins (MUC5AC and MUC1), EV biogenesis markers (TSG101), membrane remodeling proteins (GM2AP), and growth factor signaling receptors (HER2, EGFR, and GPC1), while EV-associated microRNAs were profiled by small RNA sequencing. The participant exhibiting greater physiological perturbation under hypoxia showed reduced pulmonary function and coordinated upregulation of EV proteins linked to epithelial activation, vesicle biogenesis, membrane remodeling, and growth-factor signaling, along with microRNAs (miR-200 family, miR-375, and the miR-17-92 cluster). In contrast, the participant maintaining more stable pulmonary function under hypoxia exhibited stable lung mechanics with minimal molecular perturbation. These findings underscore electrochemical uEV profiling as a sensitive and non-invasive approach for proteome-level assessment of individual-specific physiological response to hypoxic stress under extreme environmental conditions. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Praween, Nusrat AU - Praween N AD - Department of Avionics, Indian Institute of Space Science and Technology, Thiruvananthapuram, India. FAU - Akshaya, M V AU - Akshaya MV AD - Department of Avionics, Indian Institute of Space Science and Technology, Thiruvananthapuram, India. FAU - Sreekumar, M G AU - Sreekumar MG AD - Department of Avionics, Indian Institute of Space Science and Technology, Thiruvananthapuram, India. FAU - Kumar, Sunil AU - Kumar S AD - Human Space Flight Center, Indian Space Research Organisation (ISRO), Bengaluru, India. FAU - Basu, Palash Kumar AU - Basu PK AD - Department of Avionics, Indian Institute of Space Science and Technology, Thiruvananthapuram, India. Electronic address: palashbasu.sensor@gmail.com. LA - eng PT - Journal Article DEP - 20260922 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 RN - 0 (Proteome) RN - 0 (MicroRNAs) SB - IM MH - *Extracellular Vesicles/metabolism/chemistry MH - Humans MH - *Proteome/analysis/metabolism MH - *Hypoxia/urine/metabolism MH - *Adaptation, Physiological MH - *Electrochemical Techniques MH - MicroRNAs MH - Proteomics OTO - NOTNLM OT - EV-Associated proteomics and microRNAs OT - Electrochemical impedance spectroscopy OT - High-altitude physiology OT - Hypoxic stress OT - Non-invasive biomarkers OT - Urinary extracellular vesicles COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 21:56 PHST- 2026/08/02 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S0003-2670(26)01246-8 [pii] AID - 10.1016/j.aca.2026.346296 [doi] PST - ppublish SO - Anal Chim Acta. 2026 Dec 1;1425:346296. doi: 10.1016/j.aca.2026.346296. Epub 2026 Sep 22. PMID- 42951531 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1425 DP - 2026 Dec 1 TI - Machine learning-assisted fluorescence immunochromatographic detection of Salmonella typhimurium in complex food matrices via tannic acid-enhanced AIE nanoprobes. PG - 346293 LID - S0003-2670(26)01243-2 [pii] LID - 10.1016/j.aca.2026.346293 [doi] AB - BACKGROUND: Rapid on-site detection of Salmonella typhimurium in complex food matrices remains challenging due to the need for fluorescent probes that maintain intense emission in aqueous environments while resisting interference from salts and pH fluctuations. In this work, we explore tannic acid (TA) as a non-covalent interfacial modifier to construct SiO(2)/TCPE/TA (STT) nanoparticles for fluorescence immunochromatographic assays (ICA). Density functional theory (DFT) and time-dependent DFT calculations reveal that TA interacts with the aggregation-induced emission luminogens (AIEgens) TCPE through cooperative pi-pi stacking and hydrogen bonding, which stabilizes the excited state, slightly narrows the energy gap, and suppresses non-radiative decay pathways. RESULTS: Guided by the theoretical predictions, we synthesize STT nanoparticles featuring uniform spherical morphology, tunable size, and a TA-enriched surface. The TA modification boosts the absolute fluorescence quantum yield from 12.91% to 30.51% and extends the fluorescence lifetime from 1.79 ns to 2.57 ns. Taking advantage of these improved photophysical properties, the STT-based ICA strip achieves a visual detection limit of 500 CFU mL(-1) and a quantitative limit of 128 CFU mL(-1) for S. typhimurium. Practical applicability is confirmed in spiked milk, chicken, and lettuce samples, with recoveries ranging from 82.75% to 119.29%. Moreover, a machine-learning classifier trained on strip images from the three food matrices attains perfect classification accuracy across five concentration levels, enabling automated and objective result reading. SIGNIFICANCE: This work presents a non-covalent confinement strategy to engineer bright and robust AIE nanoprobes. By combining mechanistic insights from DFT calculations with experimental validation, the TA-mediated approach not only enhances fluorescence performance but also ensures matrix tolerance. The successful integration of STT nanoparticles into a lateral flow immunoassay, coupled with machine-learning-assisted interpretation, offers a rapid, sensitive, and user-friendly platform for foodborne pathogen surveillance, with strong potential for point-of-need applications. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Ji, Chenxin AU - Ji C AD - College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi, 712100, China. FAU - Li, Yuechun AU - Li Y AD - College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi, 712100, China. FAU - Cheng, Yuanyuan AU - Cheng Y AD - College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi, 712100, China. FAU - Cui, Zhaowen AU - Cui Z AD - College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi, 712100, China. FAU - Sun, Jing AU - Sun J AD - Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, 23 Xining Road, Xining, Qinghai, 810008, China. FAU - Hao, Guo AU - Hao G AD - Fuping County Inspection and Testing Center, Fuping, Shaanxi, 711799, China. FAU - Ji, Yanwei AU - Ji Y AD - College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi, 712100, China. Electronic address: jiyanwei@nwsuaf.edu.cn. FAU - Wang, Jianlong AU - Wang J AD - College of Food Science and Engineering, Northwest A&F University, 22 Xinong Road, Yangling, Shaanxi, 712100, China. Electronic address: wanglong79@nwsuaf.edu.cn. LA - eng PT - Journal Article DEP - 20260918 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 RN - 0 (Tannins) RN - 0 (Fluorescent Dyes) RN - 7631-86-9 (Silicon Dioxide) RN - 0 (tannic acid) RN - 0 (Polyphenols) SB - IM MH - *Salmonella typhimurium/isolation & purification MH - *Tannins/chemistry MH - *Nanoparticles/chemistry MH - *Machine Learning MH - *Fluorescent Dyes/chemistry MH - *Chromatography, Affinity/methods MH - Animals MH - *Food Microbiology MH - Fluorescence MH - Food Contamination/analysis MH - Limit of Detection MH - Silicon Dioxide/chemistry MH - Polyphenols OTO - NOTNLM OT - Aggregation-induced emission OT - Food safety OT - Immunochromatographic assays OT - Salmonella typhimurium OT - Tannic acid COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:56 PHST- 2026/06/14 00:00 [received] PHST- 2026/08/29 00:00 [revised] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S0003-2670(26)01243-2 [pii] AID - 10.1016/j.aca.2026.346293 [doi] PST - ppublish SO - Anal Chim Acta. 2026 Dec 1;1425:346293. doi: 10.1016/j.aca.2026.346293. Epub 2026 Sep 18. PMID- 42951529 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1425 DP - 2026 Dec 1 TI - Logic-gated in situ assembly of membrane-anchored G-quadruplex potassium channels for cancer cell apoptosis. PG - 346281 LID - S0003-2670(26)01231-6 [pii] LID - 10.1016/j.aca.2026.346281 [doi] AB - BACKGROUND: Precise recognition of the tumor microenvironment (TME) coupled with selective activation of therapeutic functions remains a major challenge in precision medicine. To address this, we present a fusogenic liposome-anchored DNA nanodevice that performs an AND logic operation in response to ATP and acidic pH and undergoes in situ assembly on the plasma membrane. RESULTS: The nanodevice comprises two transmembrane DNA strands (S1 and S2) containing pH-responsive i-motif switches and split G-quadruplex segments that are sequestered by ATP-binding aptamers. Upon fusion with the cell membrane, TME-specific stimuli trigger the structural reconfiguration of the nanodevice. Acidic conditions induce i-motif folding, bringing the two strands into proximity, while ATP binding displaces the aptamer locks. Under acidic and ATP-rich conditions, aptamer release and i-motif folding jointly promote membrane-localized assembly of a G-quadruplex structure. The nanodevice showed a ratiometric fluorescence response to pH and ATP, including a linear ATP response from 0.2 to 1.0 mM with a detection limit of 0.097 mM. The assembled G-quadruplex structure acted as an artificial transmembrane ion channel, induced intracellular K(+) depletion, mitochondrial membrane depolarization, caspase-3 activation, and apoptosis. SIGNIFICANCE: This work provides a proof-of-concept strategy for logic-gated ion interference under tumor-mimicking extracellular conditions, highlighting the utility of DNA nanodevices in translating multiple microenvironmental stimuli into targeted cellular perturbations. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Feng, Lei AU - Feng L AD - Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, College of Chemistry and Molecular Sciences, Anhui Normal University, Wuhu, 241002, PR China. FAU - Wang, Qiuting AU - Wang Q AD - Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, College of Chemistry and Molecular Sciences, Anhui Normal University, Wuhu, 241002, PR China. FAU - Xu, Wenwen AU - Xu W AD - Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, College of Chemistry and Molecular Sciences, Anhui Normal University, Wuhu, 241002, PR China. FAU - Ling, Pinghua AU - Ling P AD - Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, College of Chemistry and Molecular Sciences, Anhui Normal University, Wuhu, 241002, PR China. Electronic address: phling@ahnu.edu.cn. LA - eng PT - Journal Article DEP - 20260917 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 RN - 0 (Aptamers, Nucleotide) RN - 0 (Potassium Channels) RN - 8L70Q75FXE (Adenosine Triphosphate) RN - 0 (Liposomes) SB - IM MH - *G-Quadruplexes MH - *Apoptosis MH - Humans MH - Hydrogen-Ion Concentration MH - Aptamers, Nucleotide/chemistry MH - *Potassium Channels/metabolism/chemistry MH - Adenosine Triphosphate/analysis/metabolism MH - *Cell Membrane/metabolism/chemistry MH - DNA Nanostructures MH - Liposomes/chemistry MH - *Logic OTO - NOTNLM OT - Apoptosis OT - Artificial ion channel OT - DNA logic gate OT - G-quadruplex OT - In situ assembly COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 21:56 PHST- 2026/09/08 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S0003-2670(26)01231-6 [pii] AID - 10.1016/j.aca.2026.346281 [doi] PST - ppublish SO - Anal Chim Acta. 2026 Dec 1;1425:346281. doi: 10.1016/j.aca.2026.346281. Epub 2026 Sep 17. PMID- 42951527 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1425 DP - 2026 Dec 1 TI - A KIT-6 mesoporous silica nanoparticle-based chemiluminescent aptasensor for sensitive detection of 8-Oxo-dG. PG - 346279 LID - S0003-2670(26)01229-8 [pii] LID - 10.1016/j.aca.2026.346279 [doi] AB - BACKGROUND: Excessive accumulation of reactive oxygen species disrupts repair mechanisms and causes irreversible damage to lipids, proteins, and DNA. A target-responsive chemiluminescence sensor was developed for the sensitive detection of 8-OxodG, an important biomarker of oxidative DNA damage. RESULTS: In this chemiluminescence sensor, acridinium ester (AE) was loaded into KIT-6 mesoporous silica nanoparticles and capping the pores with an 8-oxodG-specific aptamer. In the presence of 8-oxodG, target-aptamer recognition induced aptamer detachment and AE release. The released AE reacted with H(2)O(2) to generate a concentration-dependent chemiluminescence signal. Under optimized conditions, the sensor exhibited a response range of 0.01-100 muM and a detection limit of 4.8 nM in PBS, by achieving a satisfactory recovery rate of 70.4-108.9% with RSD of 12.7-20.0%. The sensor also showed good selectivity and was successfully evaluated in spiked artificial urine with detection limit of 22.03 nM. SIGNIFICANCE: The combination of aptamer-controlled molecular gating, the interconnected pore structure of KIT-6, and chemiluminescent signal generation provides a sensitive controlled-release platform for 8-oxodG detection and supports its further development for biological-sample analysis. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Imani, Maryam AU - Imani M AD - Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University, Tehran, 14115-154, Iran. FAU - Vaezi, Zahra AU - Vaezi Z AD - Department of Biomaterial, Faculty of Interdisciplinary Sciences and Technologies, Tarbiat Modares University, Tehran, 14115-154, Iran. Electronic address: zahra.vaezi@modares.ac.ir. FAU - Naderi-Manesh, Hossein AU - Naderi-Manesh H AD - Department of Biophysics, Faculty of Biological Science, Tarbiat Modares University, Tehran, 14115-154, Iran. Electronic address: naderman@modares.ac.ir. LA - eng PT - Journal Article DEP - 20260916 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 RN - 7631-86-9 (Silicon Dioxide) RN - 0 (Aptamers, Nucleotide) RN - 88847-89-6 (8-Hydroxy-2'-Deoxyguanosine) RN - G9481N71RO (Deoxyguanosine) SB - IM MH - *Silicon Dioxide/chemistry MH - *Nanoparticles/chemistry MH - *Aptamers, Nucleotide/chemistry MH - *Luminescent Measurements/methods MH - 8-Hydroxy-2'-Deoxyguanosine MH - Porosity MH - *Biosensing Techniques/methods MH - Limit of Detection MH - *Deoxyguanosine/analogs & derivatives/analysis/urine MH - Humans OTO - NOTNLM OT - 8-oxodG OT - Aptamer OT - Biosensor OT - Chemiluminescence OT - KIT-6 OT - Mesoporous silica nanoparticles COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 21:56 PHST- 2026/07/06 00:00 [received] PHST- 2026/09/06 00:00 [revised] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S0003-2670(26)01229-8 [pii] AID - 10.1016/j.aca.2026.346279 [doi] PST - ppublish SO - Anal Chim Acta. 2026 Dec 1;1425:346279. doi: 10.1016/j.aca.2026.346279. Epub 2026 Sep 16. PMID- 42951522 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1425 DP - 2026 Dec 1 TI - Selective enrichment and profiling of GlcNAc-containing oligosaccharides in Ophiocordyceps sinensis (Berk.) across five developmental stages using WGA-functionalized NH(2)-MIL-125. PG - 346273 LID - S0003-2670(26)01223-7 [pii] LID - 10.1016/j.aca.2026.346273 [doi] AB - Glycosaminoglycans (GAGs)-like oligosaccharides in Ophiocordyceps sinensis (O. sinensis) are potential mediators of fungus-host interactions. However, their characterization is severely hindered by co-existing neutral saccharides and the low abundance N-acetylglucosamine (GlcNAc)-containing species (0.67%) of total monosaccharide. To overcome this matrix interference, we engineered a dual-interaction affinity platform (NH(2)-MIL-125-WGA) by covalently immobilizing Wheat Germ Agglutinin (WGA) onto an amino-functionalized titanium metal-organic framework. The optimized material achieved a high WGA loading capacity of 413.14 +/- 16.93 mug/mg and maintained over 80% recovery of target acidic glycans, even facing a 1000-fold excess of neutral sugars. The elimination of matrix suppression enabled the identification of 52 previously undetectable GAG-like oligosaccharides in O. sinensis via tandem mass spectrometry. Furthermore, comparative glycomic profiling across five developmental stages (mycelium, zombie larva, fruiting bodies, and spore spread period) revealed that highly sulfated and polymerized oligosaccharides were significantly upregulated during the infective mycelium stage but were notably downregulated during the transition to the reproductive fruiting body stage. These findings demonstrate the capability of the NH(2)-MIL-125-WGA platform for trace glycan analysis and suggest that dynamic variations in GAG content and sulfation patterns are critical to the fungal life cycle. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Liu, Qing-Hua AU - Liu QH AD - State Key Laboratory of Mechanism and Quality of Chinese Medicine, Guangdong-Macao Joint Laboratory for Innovative Drug Research on TCM and Natural-Derived Small RNAs, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macao Special Administrative Region of China. FAU - Yang, Liu AU - Yang L AD - State Key Laboratory of Mechanism and Quality of Chinese Medicine, Guangdong-Macao Joint Laboratory for Innovative Drug Research on TCM and Natural-Derived Small RNAs, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macao Special Administrative Region of China. FAU - Wang, Cai-Yun AU - Wang CY AD - State Key Laboratory of Mechanism and Quality of Chinese Medicine, Guangdong-Macao Joint Laboratory for Innovative Drug Research on TCM and Natural-Derived Small RNAs, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macao Special Administrative Region of China. FAU - Zhu, Guo-Yuan AU - Zhu GY AD - State Key Laboratory of Mechanism and Quality of Chinese Medicine, Guangdong-Macao Joint Laboratory for Innovative Drug Research on TCM and Natural-Derived Small RNAs, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macao Special Administrative Region of China. FAU - Bai, Li-Ping AU - Bai LP AD - State Key Laboratory of Mechanism and Quality of Chinese Medicine, Guangdong-Macao Joint Laboratory for Innovative Drug Research on TCM and Natural-Derived Small RNAs, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macao Special Administrative Region of China. FAU - Qian, Zheng-Ming AU - Qian ZM AD - Key Laboratory of State Administration of Traditional Chinese Medicine, Dongguan HEC Cordyceps R&D Co., Ltd., Dongguan, Guangdong Province, China. FAU - Dong, Lin-Lin AU - Dong LL AD - Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China. FAU - Jiang, Zhi-Hong AU - Jiang ZH AD - State Key Laboratory of Mechanism and Quality of Chinese Medicine, Guangdong-Macao Joint Laboratory for Innovative Drug Research on TCM and Natural-Derived Small RNAs, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macao Special Administrative Region of China. Electronic address: zhjiang@must.edu.mo. FAU - Zhang, Wei AU - Zhang W AD - State Key Laboratory of Mechanism and Quality of Chinese Medicine, Guangdong-Macao Joint Laboratory for Innovative Drug Research on TCM and Natural-Derived Small RNAs, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macao Special Administrative Region of China. Electronic address: wzhang@must.edu.mo. LA - eng PT - Journal Article DEP - 20260921 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 RN - 0 (Oligosaccharides) RN - V956696549 (Acetylglucosamine) RN - 0 (Wheat Germ Agglutinins) SB - IM MH - *Oligosaccharides/analysis/chemistry/metabolism MH - *Acetylglucosamine/chemistry/analysis MH - *Wheat Germ Agglutinins/chemistry MH - *Hypocreales/chemistry/growth & development OTO - NOTNLM OT - Enrichment OT - Glycosaminoglycans OT - NH(2)-MIL-125 OT - Ophiocordyceps sinensis OT - Wheat Germ Agglutinin COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:56 PHST- 2026/06/20 00:00 [received] PHST- 2026/09/05 00:00 [revised] PHST- 2026/09/14 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S0003-2670(26)01223-7 [pii] AID - 10.1016/j.aca.2026.346273 [doi] PST - ppublish SO - Anal Chim Acta. 2026 Dec 1;1425:346273. doi: 10.1016/j.aca.2026.346273. Epub 2026 Sep 21. PMID- 42951519 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1425 DP - 2026 Dec 1 TI - De novo development strategy for a robust immunocapture-LC-MS/MS assay to quantify soluble fibrinogen-like protein 1 (FGL1) as a potential clinical biomarker for immunotherapy drug development. PG - 346269 LID - S0003-2670(26)01219-5 [pii] LID - 10.1016/j.aca.2026.346269 [doi] AB - De novo development of immunocapture-LC-MS/MS assays to quantify low-abundance protein biomarkers remains challenging due to requirements for high sensitivity, specificity, and robustness. Assay development is often complicated by uncertainties associated with post-translational modifications (PTMs), surrogate peptide selection, antibody performance, and unknown endogenous concentration ranges. To address these challenges, we developed a systematic, four-stage de novo development strategy consisting of: (1) in silico digestion to identify surrogate peptide candidates; (2) optimization of LC-MS/MS conditions and enzymatic digestion conditions; (3) confirmation of antibody performance against endogenous proteins; and (4) evaluation of endogenous protein concentrations. With this strategy, we developed, to our knowledge, the first immunocapture-LC-MS/MS assay to quantify total soluble fibrinogen-like protein 1 (FGL1) in human serum, a major ligand of lymphocyte activation gene-3 (LAG-3) involved in T-cell suppression. The assay utilized IRPNDFIPNVI as the surrogate peptide and mouse serum as the surrogate matrix. After immunocapture enrichment with an anti-FGL1 antibody, the surrogate peptide and internal standard (IS) were separated by reversed-phase UHPLC and detected on a SCIEX Triple Quad 6500+ system using MRM transitions of m/z 649.4 --> 856.5 for IRPNDFIPNVI peptide and m/z 652.4 --> 856.5 for IRPNDFIPNVAL((13)C(5),(15)N)I as the internal standard. The assay achieved a calibration range of 10-1000 ng/mL with sufficient sensitivity to enable quantification of FGL1 in serum samples collected from cancer patients. Fit-for-purpose validation using analytical and endogenous quality control samples showed intra-assay precision 96% of Cr(VI) within 24 h while displaying negligible Cr(III) accumulation. Performance matched theoretical predictions across pH 3.50-9.52 and ionic strengths of 0.1-100 mmol L(-1) NaNO(3). Blank values were below 0.028 mug L(-1) and method detection limits under 0.017 mug L(-1) for a 1-day deployment. Coupling the 500NFR binding gel with a planar optode enabled simultaneous high-resolution two-dimensional imaging of Cr(VI) and O(2) in the rice rhizosphere, revealing localized Cr(VI) depletion zones within 2.5 mm of the root axis and excellent spatial uniformity (RSD <10%). SIGNIFICANCE AND NOVELTY: This work establishes the 500NFR-DGT as a robust and selective platform for high-resolution in situ mapping of Cr(VI), overcoming heterogeneity limitations of conventional DGT binding gels. The integration of NFR with a planar optode enables simultaneous imaging of Cr(VI) and O(2), providing a powerful tool to elucidate redox-driven Cr dynamics in the rhizosphere and inform remediation strategies for contaminated soils. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Liu, Zhaodong AU - Liu Z AD - State Key Laboratory of Water Pollution Control and Green Resource Recycling, School of Environment, Nanjing University, Nanjing, 210023, China; State Key Laboratory of Nutrient Use and Management, Key Laboratory of Agro-Environment of Huang-Huai-Hai Plain, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Environment, Shandong Academy of Agricultural Sciences, Jinan, 250100, China. FAU - Williams, Paul N AU - Williams PN AD - Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, David Keir Building, Malone Road, Belfast, Northern Ireland, BT9 5BN, UK. FAU - Yang, Danxing AU - Yang D AD - State Key Laboratory of Water Pollution Control and Green Resource Recycling, School of Environment, Nanjing University, Nanjing, 210023, China. FAU - Luo, Jun AU - Luo J AD - State Key Laboratory of Water Pollution Control and Green Resource Recycling, School of Environment, Nanjing University, Nanjing, 210023, China. Electronic address: esluojun@nju.edu.cn. LA - eng PT - Journal Article DEP - 20260910 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 SB - IM OTO - NOTNLM OT - Diffusive gradients in thin-films (DGT) OT - Hexavalent chromium (Cr(VI)) OT - High-resolution chemical imaging OT - In situ speciation analysis OT - Rice rhizosphere COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:56 PHST- 2026/05/20 00:00 [received] PHST- 2026/08/17 00:00 [revised] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S0003-2670(26)01198-0 [pii] AID - 10.1016/j.aca.2026.346248 [doi] PST - ppublish SO - Anal Chim Acta. 2026 Dec 1;1425:346248. doi: 10.1016/j.aca.2026.346248. Epub 2026 Sep 10. PMID- 42951506 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1873-4324 (Electronic) IS - 0003-2670 (Linking) VI - 1425 DP - 2026 Dec 1 TI - The early MALDI years and how "microscopy-grade" transmission-mode MALDI-2 mass spectrometry imaging was realized after a long delay: a personal retrospective and review. PG - 346153 LID - S0003-2670(26)01103-7 [pii] LID - 10.1016/j.aca.2026.346153 [doi] AB - Since its invention in the mid-1980s, matrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) has developed into a key bioanalytical technique for numerous research areas in the life sciences. One remarkable application shift that began in the mid-1990s is the increasing use of the label-free MALDI technique as a molecular microscope. One holy grail of MALDI-MS Imaging (MALDI-MSI) is to reach a true single-cell or even a sub-cellular resolution to visualize the molecular content of individual cells and organelles thereof. This requires pixel sizes in the low to sub-micrometer range. These are difficult to achieve, such that new irradiation concepts are needed. Another contemporary research focus is on the advancement of multimodal imaging, especially in the combination of immunofluorescence (IF) microscopy and highly-resolved MALDI-MSI. Drawing upon four decades of active research in the MALDI field, I structured this review to offer both a personal (retro-)perspective and a comprehensive exploration of both historical and contemporary aspects. The initial chapter provides a historical overview of the development of the first laser microprobe mass analyzer (LAMMA 500), while the subsequent chapter chronicles the discovery of the MALDI technique and its initial applications in biological research. The third and fourth sections then direct the reader's attention to selected recent advancements in MALDI-imaging, highlighting pioneering contributions from both in-house and external laboratories. These developments include the utilization of laser postionization for MALDI-2 and the resurgence of the LAMMA 500 concept in the form of transmission (t-) mode MALDI-2. The recently introduced direct coupling of t-MALDI-2 with IF microscopy holds significant promise in unveiling novel avenues for cell biology research. CI - Copyright (c) 2026 The Author. Published by Elsevier B.V. All rights reserved. FAU - Dreisewerd, Klaus AU - Dreisewerd K AD - University of Munster, Institute of Hygiene, Domagkstr. 40, Munster, 48149, Germany. Electronic address: Klaus.dreisewerd@uni-muenster.de. LA - eng PT - Historical Article PT - Journal Article PT - Review DEP - 20260825 PL - Netherlands TA - Anal Chim Acta JT - Analytica chimica acta JID - 0370534 SB - IM MH - *Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods/history/instrumentation MH - Humans MH - History, 20th Century MH - Animals MH - History, 21st Century OTO - NOTNLM OT - LAMMA 500 OT - MALDI OT - Mass spectrometry imaging OT - Multimodal imaging OT - Subcellular resolution OT - t-MALDI-2 COIS- Declaration of competing interest The author is co-inventor of patents on the MALDI technique. EDAT- 2026/10/28 23:27 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 21:56 PHST- 2026/05/21 00:00 [received] PHST- 2026/08/17 00:00 [revised] PHST- 2026/08/18 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S0003-2670(26)01103-7 [pii] AID - 10.1016/j.aca.2026.346153 [doi] PST - ppublish SO - Anal Chim Acta. 2026 Dec 1;1425:346153. doi: 10.1016/j.aca.2026.346153. Epub 2026 Aug 25. PMID- 42951494 OWN - NLM STAT- In-Process LR - 20261028 IS - 1875-6263 (Electronic) IS - 1028-4559 (Linking) VI - 65 IP - 6 DP - 2026 Nov TI - The role of laparoscopic and robotic surgery in epithelial ovarian cancer: A consensus statement of the Taiwan Association of Minimally Invasive Gynecology (TAMIG). PG - 1276-1278 LID - S1028-4559(26)00305-0 [pii] LID - 10.1016/j.tjog.2026.08.021 [doi] FAU - Wang, Peng-Hui AU - Wang PH AD - Department of Obstetrics and Gynecology, Taipei Veterans General Hospital and National Yang Ming Chiao Tung University, Taipei, Taiwan; Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia. FAU - Yen, Chih-Feng AU - Yen CF AD - Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital and University, Tao-Yuan, Taiwan. FAU - Huang, Kuan-Gen AU - Huang KG AD - Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital and University, Tao-Yuan, Taiwan. FAU - Wang, Kung-Liahng AU - Wang KL AD - Department of Obstetrics and Gynecology, MacKay Memorial Hospital, Taipei City, Taiwan. FAU - Chen, Kuo-Hu AU - Chen KH AD - Department of Obstetrics and Gynecology, Taipei Tzu-Chi Hospital, The Buddhist Tzu-Chi Medical Foundation, Taipei, Taiwan and School of Medicine, Tzu-Chi University, Hualien, Taiwan. FAU - Lin, Yu-Shan AU - Lin YS AD - Department of Obstetrics and Gynecology, Jen-Ai Hospital Dali Branch, Taichung, Taiwan. FAU - Lee, Chyi-Long AU - Lee CL AD - Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital and University, Tao-Yuan, Taiwan. Electronic address: leechyilong@gmail.com. CN - Board of Directors and consultants of the Taiwan Association of Minimally Invasive Gynecology (TAMIG) LA - eng PT - Letter PL - China (Republic : 1949- ) TA - Taiwan J Obstet Gynecol JT - Taiwanese journal of obstetrics & gynecology JID - 101213819 SB - IM COIS- Conflicts of interest Drs. Peng-Hui Wang, Kung-Liahng Wang, and Chyi-Long Lee, editorial board members at Taiwanese Journal of Obstetrics and Gynecology, had no role in the peer review process of or decision to publish this article. The authors declare that they have no conflicts of interest related to the subject matter or materials discussed in this article. FIR - Lin, Wu-Chou IR - Lin WC IRAD- China Medical University Hospital, Taichung, Taiwan. FIR - Huang, Kuan-Hui IR - Huang KH IRAD- Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan. FIR - Chang, Chi-Chang IR - Chang CC IRAD- E-Da Dachang Hospital, Kaohsiung, Taiwan. FIR - Wu, Ming-Ping IR - Wu MP IRAD- Chi Mei Medical Center, Tainan, Taiwan. FIR - Wu, Hsin-Hong IR - Wu HH IRAD- Changhua Christian Hospital, Changhua, Taiwan. FIR - Huang, Ming-Chao IR - Huang MC IRAD- Mackay Memorial Hospital, Taipei, Taiwan. FIR - Sun, Chung-Hsien IR - Sun CH IRAD- Lucina Woman and Children Hospital, Kaohsiung, Taiwan. FIR - Long, Cheng-Yu IR - Long CY IRAD- Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung, Taiwan. FIR - Chang, Yu IR - Chang Y IRAD- Kaohsiung Show Chwan Memorial Hospital, Kaohsiung, Taiwan. FIR - Sheu, Bor-Ching IR - Sheu BC IRAD- National Sun Yat-Sen University, Kaohsiung, Taiwan. FIR - Kay, Nari IR - Kay N IRAD- E-Da Hospital, Kaohsiung, Taiwan. FIR - Chen, Yi-Jen IR - Chen YJ IRAD- Taipei Veterans General Hospital, Taipei, Taiwan. FIR - Tsui, Kuan-Hao IR - Tsui KH IRAD- Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan. FIR - Ou, Yu-Che IR - Ou YC IRAD- Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan. FIR - Wu, Pei-Ju IR - Wu PJ IRAD- Chung Shan Medical University Hospital, Taichung, Taiwan. FIR - Kuo, Hsin-Hong IR - Kuo HH IRAD- Hungchi Women's and Children's Hospital, Tao-Yuan, Taiwan. FIR - We, Meng-Hsing IR - We MH IRAD- National Cheng Kung University Hospital, Tainan, Taiwan. FIR - Cheng, Ya-Min IR - Cheng YM IRAD- Kuo General Hospital, Tainan, Taiwan. FIR - Tzeng, Chii-Ruey IR - Tzeng CR IRAD- TFC Taipei Fertility Center, Taipei, Taiwan. FIR - Chang, Yin-Yi IR - Chang YY IRAD- China Medical University Hospital, Taichung, Taiwan. FIR - Chuang, Fei-Chi IR - Chuang FC IRAD- Fei-Chi Chuang Women's Clinics, Kaohsiung, Taiwan. FIR - Ding, Dah-Ching IR - Ding DC IRAD- Hualien Tzu Chi Hospital, Hualien, Taiwan. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 21:56 PHST- 2026/08/18 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S1028-4559(26)00305-0 [pii] AID - 10.1016/j.tjog.2026.08.021 [doi] PST - ppublish SO - Taiwan J Obstet Gynecol. 2026 Nov;65(6):1276-1278. doi: 10.1016/j.tjog.2026.08.021. PMID- 42951472 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1875-6263 (Electronic) IS - 1028-4559 (Linking) VI - 65 IP - 6 DP - 2026 Nov TI - Frequent blastocyst contractions with area reduction >30% are associated with reduced embryo quality but not IVF outcomes: A retrospective cohort study of fresh and frozen single embryo transfer. PG - 1191-1197 LID - S1028-4559(26)00285-8 [pii] LID - 10.1016/j.tjog.2026.08.007 [doi] AB - OBJECTIVE: This study aimed to analyze the effect of blastocyst contraction on embryo quality and subsequent in vitro fertilization (IVF) outcomes in women undergoing fresh single embryo transfer (SET) and frozen SET. MATERIAL AND METHODS: This retrospective cohort study included 300 patients who underwent 343 oocyte retrievals at Mackay Memorial Hospital between February 1, 2022, and February 29, 2024. The oocytes were divided into two categories based on whether blastocyst contraction occurred. We further analyzed the blastocyst morphology grading and KIDScore. We also analyzed subsequent fresh and frozen SET outcomes. Furthermore, we conducted a subgroup analysis for the frequency of contraction number and embryo quality. RESULTS: In all embryo analyses, the contraction group was associated with significantly lower morphology grading (good embryos 25.7% vs. 68.9%, fair embryos 27.4% vs. 4.3%, poor embryos 46.9% vs. 26.8%, p < 0.001) and lower KIDScore (>==8, 14.3% vs. 20.2%, >== 6 and < 8, 25.5% vs. 28.2%, <6, 60.1% vs. 51.6%, p = 0.020) compared to the non-contraction group. In the contraction number analysis, higher numbers (3-4 and >== 5 times) were associated with significantly poorer embryo quality than less numbers (1-2 times) (p < 0.001). However, in the fresh and frozen-thawed SET analyses, there were no significant differences in the clinical pregnancy, ongoing pregnancy, livebirth, and miscarriage rates between the contraction and non-contraction groups. CONCLUSION: The occurrence of blastocyst contractions-particularly at higher frequencies-was associated with poorer embryo quality. However, in the analysis of fresh and frozen-thawed SET, the clinical pregnancy, ongoing pregnancy, livebirth, and miscarriage rates were not significantly different between both groups, indicating that although contraction may reflect underlying differences in embryo viability, its impact on IVF success is minimal when high-quality embryos are selected rigorously for transfer. However, large research is needed to validate this conclusion. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Lee, Chia-Chen AU - Lee CC AD - Division of Reproduction and Infertility, Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taiwan. FAU - Lee, Kuan-Sheng AU - Lee KS AD - Division of Reproduction and Infertility, Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taiwan. FAU - Chiu, Christine Shan-Chi AU - Chiu CS AD - Division of Reproduction and Infertility, Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taiwan. FAU - Lee, Robert Kuo-Kuang AU - Lee RK AD - Division of Reproduction and Infertility, Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taiwan. FAU - Lin, Ming-Huei AU - Lin MH AD - Division of Reproduction and Infertility, Department of Obstetrics and Gynecology, Mackay Memorial Hospital, Taiwan. Electronic address: huei97@mmh.org.tw. LA - eng PT - Journal Article PL - China (Republic : 1949- ) TA - Taiwan J Obstet Gynecol JT - Taiwanese journal of obstetrics & gynecology JID - 101213819 SB - IM MH - Humans MH - Female MH - Retrospective Studies MH - Pregnancy MH - *Fertilization in Vitro/methods/statistics & numerical data MH - Adult MH - Cryopreservation MH - *Blastocyst/physiology MH - *Single Embryo Transfer/methods MH - Pregnancy Rate MH - Oocyte Retrieval OTO - NOTNLM OT - Blastocyst OT - Embryo quality OT - Fertilization in vitro OT - Oocyte retrieval OT - Single embryo transfer OT - Spontaneous Abortion COIS- Declarations of interest None. EDAT- 2026/10/28 23:27 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 21:56 PHST- 2025/08/28 00:00 [accepted] PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S1028-4559(26)00285-8 [pii] AID - 10.1016/j.tjog.2026.08.007 [doi] PST - ppublish SO - Taiwan J Obstet Gynecol. 2026 Nov;65(6):1191-1197. doi: 10.1016/j.tjog.2026.08.007. PMID- 42951463 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1875-6263 (Electronic) IS - 1028-4559 (Linking) VI - 65 IP - 6 DP - 2026 Nov TI - Struma ovarii: Diagnostic pitfalls, management strategies, and clinical outcomes from a tertiary medical center. PG - 1133-1140 LID - S1028-4559(26)00276-7 [pii] LID - 10.1016/j.tjog.2026.08.004 [doi] AB - OBJECTIVE: To summarize the clinical, imaging, pathological, and surgical characteristics of struma ovarii and evaluate outcomes from a 17-year single-center experience. MATERIALS AND METHODS: A retrospective review of 35 patients with histologically confirmed struma ovarii treated at Cathay General Hospital between January 2008 and January 2025 was conducted. Clinical data, imaging features, surgical approach, frozen section use, pathology, and follow-up outcomes were analyzed. RESULTS: The mean age at diagnosis was 43.3 years. One-third of patients were asymptomatic; others presented with abdominal pain, distension, or pelvic mass. Mean CA-125 was 51.5 U/mL. Ultrasonography commonly revealed multiloculated cystic masses with heterogeneous echotexture and minimal Doppler flow; several cases exhibited the characteristic "struma pearl sign." Laparoscopic surgery was performed in 60% of patients, and frozen section in 57%. Pathology showed 18 benign pure, 15 benign impure, and 2 malignant cases with papillary thyroid carcinoma arising within struma ovarii. During a median follow-up of 18 months, no recurrence or metastasis occurred. CONCLUSION: Struma ovarii is a rare ovarian teratoma with variable clinical and imaging presentations. The struma pearl sign, when present, may serve as a supportive sonographic clue, but its absence does not exclude the diagnosis. Frozen section may assist intraoperative decision-making in selected cases, although final diagnosis should rely on permanent histopathological evaluation. Long-term follow-up remains important, particularly for malignant or atypical cases. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Cheng, I-Ning AU - Cheng IN AD - Department of Women's Medicine, Cathay General Hospital, Taipei, Taiwan. FAU - Chen, Wei-An AU - Chen WA AD - Department of Women's Medicine, Cathay General Hospital, Taipei, Taiwan; Graduate Institute of Biomedical Informatics, College of Medical Science and Technology, Taipei Medical University, Taipei City, Taiwan. FAU - Tseng, Yu-Ju AU - Tseng YJ AD - Department of Women's Medicine, Cathay General Hospital, Taipei, Taiwan. FAU - Ho, Chih-Ming AU - Ho CM AD - Department of Women's Medicine, Cathay General Hospital, Taipei, Taiwan; School of Medicine, Fu Jen Catholic University, Hsin-Chuang, New Taipei City, Taiwan. FAU - Chien, Tsai-Yen AU - Chien TY AD - Department of Women's Medicine, Cathay General Hospital, Taipei, Taiwan. FAU - Huang, Chia-Yen AU - Huang CY AD - Department of Women's Medicine, Cathay General Hospital, Taipei, Taiwan; School of Medicine, Fu Jen Catholic University, Hsin-Chuang, New Taipei City, Taiwan; Department of Obstetrics and Gynecology, Sijhih Cathay General Hospital, New Taipei City, Taiwan. Electronic address: cgh11360@cgh.org.tw. LA - eng PT - Journal Article PL - China (Republic : 1949- ) TA - Taiwan J Obstet Gynecol JT - Taiwanese journal of obstetrics & gynecology JID - 101213819 RN - 0 (CA-125 Antigen) SB - IM MH - Humans MH - Female MH - *Struma Ovarii/surgery/diagnosis/pathology/diagnostic imaging MH - *Ovarian Neoplasms/surgery/diagnosis/pathology/diagnostic imaging MH - Retrospective Studies MH - Adult MH - Middle Aged MH - Tertiary Care Centers MH - Ultrasonography MH - Laparoscopy MH - Young Adult MH - CA-125 Antigen/blood MH - Frozen Sections MH - Thyroid Cancer, Papillary/pathology MH - Aged MH - Treatment Outcome MH - Adolescent OTO - NOTNLM OT - Frozen section OT - Ovarian teratoma OT - Papillary thyroid carcinoma OT - Retrospective study OT - Struma ovarii OT - Thyroid tissue OT - Ultrasound COIS- Declaration of competing interest The authors have no conflicts of interest relevant to this article. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:56 PHST- 2026/06/24 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S1028-4559(26)00276-7 [pii] AID - 10.1016/j.tjog.2026.08.004 [doi] PST - ppublish SO - Taiwan J Obstet Gynecol. 2026 Nov;65(6):1133-1140. doi: 10.1016/j.tjog.2026.08.004. PMID- 42951462 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1875-6263 (Electronic) IS - 1028-4559 (Linking) VI - 65 IP - 6 DP - 2026 Nov TI - Protective effects of melatonin against bisphenol A-mediated endometrial tumorigenesis: An experimental study targeting COX-2-dependent MMP-9 expression. PG - 1126-1132 LID - S1028-4559(26)00275-5 [pii] LID - 10.1016/j.tjog.2026.04.019 [doi] AB - OBJECTIVE: Bisphenol A (BPA), a widespread environmental endocrine disruptor, contributes to the development of endometrial carcinogenesis by activating the cyclooxygenase-2 (COX-2)/matrix metalloproteinase-9 (MMP-9) axis. In this study, the protective effects of melatonin on BPA-induced protumorigenic alterations were evaluated in the human endometrial carcinoma cell line RL95-2. MATERIALS AND METHODS: RL95-2 cells were treated with BPA (10(-7) M) alone or in combination with varying concentrations of melatonin (10(-10) to 10(-4) M) for 24 h. An MTT assay was performed to assess cell viability. RT-PCR and Western blotting were performed to evaluate COX-2 and MMP-9 expression. Immunofluorescence and flow cytometry were performed to analyze protein localization and quantify the COX-2(+)/MMP-9(+) cell population. Cells were treated with NS-398, a selective COX-2 inhibitor, to validate COX-2 dependency. RESULTS: BPA significantly increased RL95-2 cell proliferation and upregulated COX-2 and MMP-9 expression. Melatonin cotreatment, particularly at 10(-6) M, suppressed BPA-induced proliferation and attenuated both COX-2 and MMP-9 expression at the gene and protein levels. Flow cytometry revealed that BPA significantly increased the proportion of COX-2(+)/MMP-9(+) cells. Both melatonin and NS-398 reversed this effect. These findings highlight the capacity of melatonin to mitigate BPA-induced proliferation and invasiveness by suppressing COX-2-mediated MMP-9 expression. CONCLUSION: Melatonin effectively mitigated BPA-induced proliferation and proinflammatory and proinvasive RL95-2 phenotypes by targeting the COX-2/MMP-9 signaling axis. These findings support the potential of melatonin as a physiologically safe therapeutic agent for preventing endometrial tumorigenesis induced by environmental endocrine disruptors, underscoring its anti-inflammatory relevance in reproductive oncology. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Chen, Tsung-Ming AU - Chen TM AD - Department of Occupational Safety and Health, Chia Nan University of Pharmacy and Science, Tainan, Taiwan; Center of General Education, Chia Nan University of Pharmacy and Science, Tainan, Taiwan. FAU - Hsuan-Yu Lin, Sean AU - Hsuan-Yu Lin S AD - Department of Laboratory Medicine, Kuo General Hospital, Tainan, Taiwan; Center for Reproductive Medicine, Kuo General Hospital, Tainan, Taiwan. FAU - Wang, Kai-Hung AU - Wang KH AD - Department of Laboratory Medicine, Kuo General Hospital, Tainan, Taiwan; Center for Reproductive Medicine, Kuo General Hospital, Tainan, Taiwan. Electronic address: kevinwang0518@yahoo.com.tw. FAU - Lin, Ta-Chin AU - Lin TC AD - Center for Reproductive Medicine, Kuo General Hospital, Tainan, Taiwan; Department of Obstetrics and Gynecology, Kuo General Hospital, Tainan, Taiwan. Electronic address: tachin25@gmail.com. FAU - Kuo, Tsung-Cheng AU - Kuo TC AD - Center for Reproductive Medicine, Kuo General Hospital, Tainan, Taiwan; Department of Obstetrics and Gynecology, Kuo General Hospital, Tainan, Taiwan. LA - eng PT - Journal Article PL - China (Republic : 1949- ) TA - Taiwan J Obstet Gynecol JT - Taiwanese journal of obstetrics & gynecology JID - 101213819 RN - MLT3645I99 (bisphenol A) RN - 0 (Phenols) RN - JL5DK93RCL (Melatonin) RN - 0 (Bisphenol A Compounds) RN - 0 (Benzhydryl Compounds) RN - EC 3.4.24.35 (Matrix Metalloproteinase 9) RN - EC 1.14.99.1 (Cyclooxygenase 2) RN - EC 3.4.24.35 (MMP9 protein, human) RN - 0 (Endocrine Disruptors) RN - EC 1.14.99.1 (PTGS2 protein, human) SB - IM MH - Female MH - Humans MH - *Phenols/toxicity/adverse effects MH - *Melatonin/pharmacology MH - Bisphenol A Compounds MH - *Benzhydryl Compounds/toxicity MH - *Matrix Metalloproteinase 9/metabolism/drug effects MH - *Cyclooxygenase 2/metabolism/drug effects MH - *Endometrial Neoplasms/chemically induced/prevention & control/pathology MH - Cell Line, Tumor MH - Cell Proliferation/drug effects MH - Endocrine Disruptors/toxicity MH - *Carcinogenesis/drug effects MH - Cell Survival/drug effects OTO - NOTNLM OT - Bisphenol A OT - COX-2 OT - MMP-9 OT - Melatonin OT - RL95-2 cells COIS- Conflicts of interest statement The authors have no conflicts of interest relevant to this article to declare. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:56 PHST- 2026/04/16 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S1028-4559(26)00275-5 [pii] AID - 10.1016/j.tjog.2026.04.019 [doi] PST - ppublish SO - Taiwan J Obstet Gynecol. 2026 Nov;65(6):1126-1132. doi: 10.1016/j.tjog.2026.04.019. PMID- 42951461 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1875-6263 (Electronic) IS - 1028-4559 (Linking) VI - 65 IP - 6 DP - 2026 Nov TI - A pilot study of electromagnetic therapy versus biofeedback-based pelvic floor muscle training for pelvic organ prolapse: A mixed-methods approach. PG - 1117-1125 LID - S1028-4559(26)00274-3 [pii] LID - 10.1016/j.tjog.2026.01.024 [doi] AB - OBJECTIVES: This pilot study aimed to: (1) evaluate the effects of high-intensity focused electromagnetic (HIFEM) therapy on pelvic organ prolapse (POP) symptoms and pelvic floor function; (2) compare outcomes across HIFEM, pelvic floor muscle training (PFMT) with biofeedback and/or electrostimulation, and usual care; and (3) explore participant acceptability of the interventions. MATERIALS AND METHODS: A mixed-methods pilot study was conducted in women aged >20 years with stage I-III POP. Participants were randomized to HIFEM, PFMT, or usual care. The HIFEM group underwent twice-weekly 28-min sessions for eight weeks using a non-invasive electromagnetic chair. PFMT consisted of supervised sessions incorporating biofeedback and/or electrostimulation. Usual care included lifestyle advice. Outcomes were assessed using validated questionnaires for POP symptoms and pelvic floor distress, digital palpation, and transperineal ultrasound. RESULTS: Twenty-eight women completed the study (HIFEM, n = 10; PFMT, n = 9; usual care, n = 9). Compared with usual care, HIFEM significantly improved pelvic floor muscle endurance (p = 0.001), whereas PFMT significantly improved muscle strength (p = 0.034), endurance (p = 0.023), and rapid contractions (p = 0.019). Only HIFEM demonstrated significant improvements in POP symptom severity (p = 0.015) and pelvic floor distress (p = 0.041). No serious adverse events were reported. Participant satisfaction was highest in the HIFEM group (100%). Qualitative analysis supported intervention acceptability, with participants reporting increased pelvic floor awareness, improved symptom control, and enhanced psychological well-being. CONCLUSIONS: Both HIFEM and PFMT with biofeedback appear to be safe, acceptable, and effective non-surgical treatment options for women with POP. Although no significant differences were observed between the two interventions, these findings should be interpreted with caution because of the small sample size. Larger studies are needed to confirm comparative efficacy and inform clinical implementation. CLINICAL TRIAL REGISTRATION NUMBER: NCT06532292. URL OF THE REGISTRATION SITE: https://clinicaltrials.gov/study/NCT06532292?cond=Pelvic%20Organ%20Prolapse&intr=high-intensity&rank=1. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Lin, Kuan-Yin AU - Lin KY AD - School and Graduate Institute of Physical Therapy, College of Medicine, National Taiwan University, 3F., No.17, Xuzhou Rd., Zhongzheng Dist., Taipei City 100, Taiwan; Department of Physical Medicine and Rehabilitation, National Taiwan University Hospital, No.7, Chung Shan S. Rd.(Zhongshan S. Rd.), Zhongzheng Dist., Taipei City 100, Taiwan. Electronic address: kuanyinl27173@ntu.edu.tw. FAU - Wang, Li-Ying AU - Wang LY AD - School and Graduate Institute of Physical Therapy, College of Medicine, National Taiwan University, 3F., No.17, Xuzhou Rd., Zhongzheng Dist., Taipei City 100, Taiwan; Physical Therapy Center, National Taiwan University Hospital, No.7, Chung Shan S. Rd.(Zhongshan S. Rd.), Zhongzheng Dist., Taipei City 100, Taiwan. FAU - Wu, Pei-Chi AU - Wu PC AD - Department of Obstetrics and Gynecology, National Taiwan University Hospital, No.7, Chung Shan S. Rd.(Zhongshan S. Rd.), Zhongzheng Dist., Taipei City 100, Taiwan. FAU - Chen, Chao-Yin AU - Chen CY AD - School and Graduate Institute of Physical Therapy, College of Medicine, National Taiwan University, 3F., No.17, Xuzhou Rd., Zhongzheng Dist., Taipei City 100, Taiwan; Physical Therapy Center, National Taiwan University Hospital, No.7, Chung Shan S. Rd.(Zhongshan S. Rd.), Zhongzheng Dist., Taipei City 100, Taiwan. FAU - Huang, Min-Hsuan AU - Huang MH AD - Physical Therapy Center, National Taiwan University Hospital, No.7, Chung Shan S. Rd.(Zhongshan S. Rd.), Zhongzheng Dist., Taipei City 100, Taiwan. LA - eng SI - ClinicalTrials.gov/NCT06532292 PT - Comparative Study PT - Journal Article PT - Randomized Controlled Trial PL - China (Republic : 1949- ) TA - Taiwan J Obstet Gynecol JT - Taiwanese journal of obstetrics & gynecology JID - 101213819 SB - IM MH - Humans MH - Female MH - Pilot Projects MH - *Pelvic Organ Prolapse/therapy/physiopathology MH - Middle Aged MH - Pelvic Floor/physiopathology MH - *Biofeedback, Psychology/methods MH - *Exercise Therapy/methods MH - *Magnetic Field Therapy/methods MH - Treatment Outcome MH - Aged MH - Adult MH - Electric Stimulation Therapy/methods OTO - NOTNLM OT - Biofeedback OT - Electromagnetic therapy OT - Pelvic floor muscle training OT - Pelvic organ prolapse COIS- Conflicts of interest statement Kuan-Yin Lin reports financial support from the National Science and Technology Council (Taiwan). All other authors declare no known competing financial interests or personal relationships that could have influenced the work reported in this article. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:56 PHST- 2026/01/20 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S1028-4559(26)00274-3 [pii] AID - 10.1016/j.tjog.2026.01.024 [doi] PST - ppublish SO - Taiwan J Obstet Gynecol. 2026 Nov;65(6):1117-1125. doi: 10.1016/j.tjog.2026.01.024. PMID- 42951460 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1875-6263 (Electronic) IS - 1028-4559 (Linking) VI - 65 IP - 6 DP - 2026 Nov TI - Learning curve and determinants of sentinel lymph node mapping outcomes in endometrial cancer: A single-center CUSUM analysis. PG - 1110-1116 LID - S1028-4559(26)00273-1 [pii] LID - 10.1016/j.tjog.2026.03.027 [doi] AB - OBJECTIVE: To evaluate the learning curve of sentinel lymph node (SLN) mapping in endometrial cancer and to identify predictors of mapping failure in a single-center experience. MATERIALS AND METHODS: We retrospectively analyzed 59 patients with clinically early-stage endometrial cancer who underwent minimally invasive staging with SLN mapping at Cathay General Hospital between August 2021 and February 2025. Cumulative bilateral detection rates and cumulative sum (CUSUM) analyses were applied to assess the learning curve. Clinical, surgical, and pathological factors associated with mapping failure were examined by univariate analysis. RESULTS: Median age was 52 years and median BMI was 27.4 kg/m(2). Endometrioid histology comprised 95% of cases, and 81% were 2023 FIGO stage I. The overall detection rate was 92.3%, with bilateral mapping in 69.5%, unilateral mapping in 23.7%, and complete failure in 6.8%. Bilateral detection improved from 65% in the early phase (cases 1-20) to 72% in the late phase (cases 21-59). CUSUM indicated proficiency after approximately 38 cases, with stabilization projected at approximately 65 cases. Observed mapping failures were often associated with inadequate dye migration or empty specimens. Adnexal adhesions showed a borderline association with bilateral mapping failure (p = 0.080), whereas age, BMI, histologic grade, lymphovascular invasion, and stage were not significant. CONCLUSION: SLN mapping in endometrial cancer exhibits a distinct learning curve, with proficiency achieved after approximately 40-65 cases. Adhesions encountered during surgery, particularly adnexal adhesions, may represent a key technical barrier, underscoring the importance of structured training, standardized protocols, and meticulous surgical management to optimize mapping outcomes. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Chen, Wei-An AU - Chen WA AD - Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei, Taiwan; Graduate Institute of Biomedical Informatics, College of Medical Science and Technology, Taipei Medical University, Taipei City, Taiwan. FAU - Tseng, Yu-Ju AU - Tseng YJ AD - Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei, Taiwan. FAU - Ho, Chih-Ming AU - Ho CM AD - Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei, Taiwan; School of Medicine, Fu Jen Catholic University, Hsin-Chuang, New Taipei City, Taiwan. FAU - Chien, Tsai-Yen AU - Chien TY AD - Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei, Taiwan. FAU - Chang, Tzu-Hao AU - Chang TH AD - Graduate Institute of Biomedical Informatics, College of Medical Science and Technology, Taipei Medical University, Taipei City, Taiwan; Clinical Big Data Research Center, Taipei Medical University Hospital, Taipei, Taiwan. FAU - Huang, Chia-Yen AU - Huang CY AD - Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei, Taiwan; School of Medicine, Fu Jen Catholic University, Hsin-Chuang, New Taipei City, Taiwan. Electronic address: bagiao2003@gmail.com. LA - eng PT - Journal Article PL - China (Republic : 1949- ) TA - Taiwan J Obstet Gynecol JT - Taiwanese journal of obstetrics & gynecology JID - 101213819 SB - IM MH - Humans MH - Female MH - *Endometrial Neoplasms/pathology/surgery MH - *Learning Curve MH - Retrospective Studies MH - Middle Aged MH - *Sentinel Lymph Node/pathology/surgery MH - *Sentinel Lymph Node Biopsy/methods MH - Neoplasm Staging MH - Lymphatic Metastasis MH - Adult MH - Aged OTO - NOTNLM OT - Adhesions OT - Endometrial cancer OT - Learning curve OT - Mapping failure OT - Sentinel lymph node COIS- Declaration of competing interest The authors have no conflicts of interest relevant to this article. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:56 PHST- 2026/03/03 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:56 [entrez] AID - S1028-4559(26)00273-1 [pii] AID - 10.1016/j.tjog.2026.03.027 [doi] PST - ppublish SO - Taiwan J Obstet Gynecol. 2026 Nov;65(6):1110-1116. doi: 10.1016/j.tjog.2026.03.027. PMID- 42951442 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1558-0474 (Electronic) IS - 0889-8545 (Linking) VI - 53 IP - 4 DP - 2026 Dec TI - Hypertension in Pregnancy and Postpartum. PG - 747-764 LID - S0889-8545(26)00065-3 [pii] LID - 10.1016/j.ogc.2026.07.003 [doi] AB - Suboptimal prepregnancy metabolic health predisposes women to hypertensive disorders of pregnancy (HDP). Prevention of HDP starts with prepregnancy metabolic health optimization. If hypertension is present during pregnancy, adequate blood pressure control becomes crucial. Pregnancy and lactation safety should be considered when selecting an antihypertensive medication regimen. Aspirin prophylaxis is recommended for women at risk of preeclampsia. HDPs and other APOs increase the risk of future maternal cardiovascular events. Therefore, continued surveillance, screening, and cardiometabolic health optimization after pregnancy are essential to improving long-term health. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Hoppe, Kara K AU - Hoppe KK AD - Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, University of Wisconsin-Madison, Madison, WI, USA. Electronic address: khoppe2@wisc.edu. FAU - Thordsen, Sarah AU - Thordsen S AD - Division of Cardiovascular Medicine, Department of Medicine, University of Wisconsin-Madison, Madison, WI, USA. LA - eng PT - Journal Article PT - Review DEP - 20260915 PL - United States TA - Obstet Gynecol Clin North Am JT - Obstetrics and gynecology clinics of North America JID - 8709551 RN - 0 (Antihypertensive Agents) RN - R16CO5Y76E (Aspirin) SB - IM MH - Humans MH - Female MH - Pregnancy MH - *Hypertension, Pregnancy-Induced/prevention & control/drug therapy/diagnosis/physiopathology MH - *Antihypertensive Agents/therapeutic use MH - Postpartum Period MH - Pre-Eclampsia/prevention & control MH - Aspirin/therapeutic use MH - Pregnancy Outcome OTO - NOTNLM OT - Adverse pregnancy outcomes OT - Cardiovascular disease OT - Hypertension OT - Postpartum OT - Pre-eclampsia OT - Preconception OT - Pregnancy COIS- Disclosures K.K. Hoppe: Grant funding: NIH, CDC, Wisconsin Partnership Program. Industry trial involvement: Marani, Ouma Health, and Deciphr. S. Thordson: None to report. EDAT- 2026/10/28 23:27 MHDA- 2026/10/29 05:30 CRDT- 2026/10/28 21:55 PHST- 2026/10/29 05:30 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:55 [entrez] AID - S0889-8545(26)00065-3 [pii] AID - 10.1016/j.ogc.2026.07.003 [doi] PST - ppublish SO - Obstet Gynecol Clin North Am. 2026 Dec;53(4):747-764. doi: 10.1016/j.ogc.2026.07.003. Epub 2026 Sep 15. PMID- 42951389 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1875-9157 (Electronic) IS - 1875-9157 (Linking) VI - 19 IP - 4 DP - 2026 Dec TI - Uterine Adenosarcoma: Unusual Presentations and Mimics. PG - 617-636 LID - S1875-9181(26)00067-X [pii] LID - 10.1016/j.path.2026.06.009 [doi] AB - Uterine adenosarcoma is an uncommon biphasic neoplasm composed of malignant stroma and benign non-neoplastic epithelium. Most adenosarcomas show canonical low-grade morphology, which must be distinguished from various benign uterine polyps. A subset of adenosarcomas shows sarcomatous overgrowth, myometrial invasion, vascular invasion, heterologous differentiation, or marked nuclear atypia. These findings are prognostically adverse and may raise the differential with other uterine sarcomas. This article provides an overview of the clinicopathologic features of uterine adenosarcoma, with a particular focus on challenging presentations and histologic mimics. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Dinh, Timothy A AU - Dinh TA AD - Department of Pathology, University of Michigan, North Campus Research Complex, 2800 Plymouth Road, Building 35, Ann Arbor, MI 48109, USA. Electronic address: timdinh@med.umich.edu. FAU - Chapel, David B AU - Chapel DB AD - Department of Pathology, University of Michigan, North Campus Research Complex, 2800 Plymouth Road, Building 35, Ann Arbor, MI 48109, USA. LA - eng PT - Journal Article PT - Review DEP - 20260805 PL - United States TA - Surg Pathol Clin JT - Surgical pathology clinics JID - 101491209 RN - Adenosarcoma of the uterus SB - IM MH - Humans MH - *Adenosarcoma/pathology/diagnosis MH - Female MH - *Uterine Neoplasms/pathology/diagnosis MH - Diagnosis, Differential MH - Carcinosarcoma/pathology/diagnosis MH - Polyps/pathology/diagnosis MH - Uterus/pathology MH - Mixed Tumor, Mullerian/pathology/diagnosis MH - Rhabdomyosarcoma/pathology/diagnosis OTO - NOTNLM OT - Adenosarcoma OT - Carcinosarcoma OT - Endometrial stromal sarcoma OT - Mixed tumor OT - Mullerian OT - Polyps OT - Rhabdomyosarcoma OT - Uterine neoplasms COIS- Disclosure Dr D.B. Chapel reports consulting work for Johnson and Johnson. Dr T.A. Dinh reports no conflicts. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:53 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:53 [entrez] AID - S1875-9181(26)00067-X [pii] AID - 10.1016/j.path.2026.06.009 [doi] PST - ppublish SO - Surg Pathol Clin. 2026 Dec;19(4):617-636. doi: 10.1016/j.path.2026.06.009. Epub 2026 Aug 5. PMID- 42951386 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1875-9157 (Electronic) IS - 1875-9157 (Linking) VI - 19 IP - 4 DP - 2026 Dec TI - Pitfalls, Problems, and Dilemmas in Applying the FIGO 2023 Staging System for Endometrial Cancer. PG - 569-587 LID - S1875-9181(26)00065-6 [pii] LID - 10.1016/j.path.2026.06.007 [doi] AB - The publication of FIGO 2023 staging for endometrial carcinoma (EC) fundamentally changed pathologic staging of EC by incorporating histologic subtypes, extent of lymphovascular space invasion, and molecular classification. In this article, the authors discuss the new staging system, the dilemmas with molecular classification, and clinical application details of histologic examination as they relate to the staging practices of surgical pathologists. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Joehlin, Amy S AU - Joehlin AS AD - Department of Pathology, Cleveland Clinic, Cleveland, OH, USA; Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH, USA. Electronic address: joehlia@ccf.org. FAU - McGregor, Stephanie M AU - McGregor SM AD - Department of Pathology & Laboratory Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA; School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA. LA - eng PT - Journal Article PT - Review DEP - 20260826 PL - United States TA - Surg Pathol Clin JT - Surgical pathology clinics JID - 101491209 SB - IM MH - Humans MH - *Endometrial Neoplasms/pathology/genetics/diagnosis/classification MH - Female MH - *Neoplasm Staging/methods MH - Neoplasm Invasiveness MH - Lymphatic Metastasis OTO - NOTNLM OT - Endometrial carcinoma OT - FIGO 2023 staging OT - Lymphovascular space invasion OT - Molecular classification COIS- Disclosure The authors have nothing to disclose. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:53 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:53 [entrez] AID - S1875-9181(26)00065-6 [pii] AID - 10.1016/j.path.2026.06.007 [doi] PST - ppublish SO - Surg Pathol Clin. 2026 Dec;19(4):569-587. doi: 10.1016/j.path.2026.06.007. Epub 2026 Aug 26. PMID- 42951385 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1875-9157 (Electronic) IS - 1875-9157 (Linking) VI - 19 IP - 4 DP - 2026 Dec TI - An Atlas-Based Approach to Assigning International Federation of Gynecology and Obstetrics Stage to Different Presentations of Cervical Cancer. PG - 553-567 LID - S1875-9181(26)00068-1 [pii] LID - 10.1016/j.path.2026.06.010 [doi] AB - The International Federation of Gynecology and Obstetrics (FIGO) staging system for cervical carcinoma provides a globally accepted framework that guides treatment decisions and prognosticates disease. The 2018 update incorporates imaging and pathologic findings into the schema, reflecting advances in diagnostic modalities and improving stage accuracy. Despite these improvements, significant information gaps remain, and assigning an accurate FIGO stage may be challenging in several situations faced by pathologists. This article uses real world case examples for various clinical situations and presentations of cervical carcinoma, focusing on a practical approach and providing strategies to ensure accurate and clinically relevant FIGO stage designation. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Hodgson, Anjelica AU - Hodgson A AD - Laboratory Medicine Program, University Health Network, 200 Elizabeth Street, Toronto, Ontario, M5G 2C4, Canada; Department of Laboratory Medicine and Pathobiology, University of Toronto, 1 King's College Circle, Toronto, Ontario, M5S 3K9, Canada. Electronic address: anjelica.hodgson@uhn.ca. FAU - Park, Kay J AU - Park KJ AD - Department of Pathology and Laboratory Medicine, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA. LA - eng PT - Journal Article PT - Review DEP - 20260811 PL - United States TA - Surg Pathol Clin JT - Surgical pathology clinics JID - 101491209 SB - IM MH - Humans MH - *Uterine Cervical Neoplasms/pathology/diagnosis MH - Female MH - *Neoplasm Staging/methods MH - Prognosis MH - Neoplasm Invasiveness OTO - NOTNLM OT - Carcinoma OT - Cervical cancer OT - Depth of invasion OT - FIGO staging OT - Measurements OT - Tumor size COIS- Disclosure The authors have nothing to disclose that relates to the content of this article. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:53 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:53 [entrez] AID - S1875-9181(26)00068-1 [pii] AID - 10.1016/j.path.2026.06.010 [doi] PST - ppublish SO - Surg Pathol Clin. 2026 Dec;19(4):553-567. doi: 10.1016/j.path.2026.06.010. Epub 2026 Aug 11. PMID- 42951384 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1875-9157 (Electronic) IS - 1875-9157 (Linking) VI - 19 IP - 4 DP - 2026 Dec TI - Diagnostic and Prognostic Roles of Universal Estrogen Receptor Testing in Endometrial Carcinoma. PG - 541-552 LID - S1875-9181(26)00064-4 [pii] LID - 10.1016/j.path.2026.06.006 [doi] AB - Estrogen receptor (ER) immunohistochemical evaluation has emerged as a key variable in the risk assessment of endometrial carcinoma. In the most common molecular subtype of endometrial carcinomas, the no specific molecular profile, ER is used in conjunction with stage, grade, and lymphovascular space invasion (LVSI) for risk assessment and guiding adjuvant treatments. Additionally, ER is a useful tool for aiding primary site and histotype assessment and has a role in predicting response to hormonal therapies for endometrial carcinoma. These applications of ER immunostaining will be reviewed. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Huvila, Jutta AU - Huvila J AD - Department of Pathology, University of Turku, Turku, Finland; Department of Pathology, Turku University Hospital, Turku, Finland. Electronic address: jutta.huvila@utu.fi. LA - eng PT - Journal Article PT - Review DEP - 20260811 PL - United States TA - Surg Pathol Clin JT - Surgical pathology clinics JID - 101491209 RN - 0 (Receptors, Estrogen) RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - *Endometrial Neoplasms/diagnosis/pathology/metabolism MH - Female MH - Prognosis MH - *Receptors, Estrogen/metabolism/analysis MH - *Biomarkers, Tumor/metabolism/analysis MH - Immunohistochemistry MH - Neoplasm Staging OTO - NOTNLM OT - Biomarker OT - Diagnostic OT - Endometrial carcinoma OT - Estrogen receptor OT - Predictive OT - Prognostic COIS- Disclosure The author has nothing to disclose. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:53 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:53 [entrez] AID - S1875-9181(26)00064-4 [pii] AID - 10.1016/j.path.2026.06.006 [doi] PST - ppublish SO - Surg Pathol Clin. 2026 Dec;19(4):541-552. doi: 10.1016/j.path.2026.06.006. Epub 2026 Aug 11. PMID- 42951383 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1875-9157 (Electronic) IS - 1875-9157 (Linking) VI - 19 IP - 4 DP - 2026 Dec TI - Predictive Biomarker Tests for Ovarian Cancer: A Primer for Pathologists. PG - 529-539 LID - S1875-9181(26)00062-0 [pii] LID - 10.1016/j.path.2026.06.004 [doi] AB - Several biomarkers play a role in treatment selection for epithelial ovarian cancer. Homologous recombination deficiency (HRD) is a biomarker reflecting predisposing germline genetic changes as well as somatically acquired changes. Tumors with HRD may be eligible for poly-ADP(ribose) polymerase inhibitor therapy in the maintenance or recurrent setting. Expression of HER2 (ERBB2 gene product) or folate receptor alpha (FOLR1 gene product), assessed using immunohistochemical assays, is a biomarker for therapy with antibody-drug conjugates. Although the biomarker landscape in ovarian cancer remains relatively limited, these markers provide meaningful diagnostic and therapeutic guidance. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Lawson, Barrett C AU - Lawson BC AD - Department of Anatomic Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. FAU - Hagemann, Ian S AU - Hagemann IS AD - Washington University School of Medicine, St Louis, MO, USA. Electronic address: hagemani@wustl.edu. LA - eng PT - Journal Article PT - Review DEP - 20260826 PL - United States TA - Surg Pathol Clin JT - Surgical pathology clinics JID - 101491209 RN - 0 (Biomarkers, Tumor) RN - EC 2.7.10.1 (Erb-b2 Receptor Tyrosine Kinases) RN - 0 (Folate Receptor 1) RN - EC 2.7.10.1 (ERBB2 protein, human) SB - IM MH - Humans MH - Female MH - *Ovarian Neoplasms/genetics/diagnosis/pathology MH - *Biomarkers, Tumor/genetics/analysis/metabolism MH - *Neoplasms, Glandular and Epithelial/genetics/diagnosis/pathology MH - Erb-b2 Receptor Tyrosine Kinases/genetics/metabolism MH - Carcinoma, Ovarian Epithelial MH - Folate Receptor 1/genetics/metabolism OTO - NOTNLM OT - Biomarkers OT - FOLR1 OT - HER2 OT - Homologous recombination OT - Ovarian cancer COIS- Disclosures Dr I.S. Hagemann discloses consulting fees from AstraZeneca. Dr B.C. Lawson has nothing to disclose. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 21:53 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:53 [entrez] AID - S1875-9181(26)00062-0 [pii] AID - 10.1016/j.path.2026.06.004 [doi] PST - ppublish SO - Surg Pathol Clin. 2026 Dec;19(4):529-539. doi: 10.1016/j.path.2026.06.004. Epub 2026 Aug 26. PMID- 42951375 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1742-7843 (Electronic) IS - 1742-7835 (Print) IS - 1742-7835 (Linking) VI - 139 IP - 6 DP - 2026 Dec TI - Peptide-Linked Bivalent Nanobody Constructs as Positive Allosteric Modulators of the Calcium-Sensing Receptor. LID - e70320 [pii] LID - 10.1111/bcpt.70320 [doi] AB - We previously developed nanobodies acting as positive allosteric modulators (PAMs) of the calcium-sensing receptor (CaSR). Here, we sought to develop more potent CaSR modulators by creating bivalent nanobody constructs using 'tail-to-head' peptide-linker conjugation. The constructs were functionally characterized at CaSR in HEK293 cells in a Ca(2+) mobilization assay. Homobivalent Nb4-Nb4 and Nb10-Nb10 constructs with linkers comprising 3, 5, 6 or 9 GGGG(S/T) repeats all displayed CaSR PAM potencies comparable with those exhibited by their monovalent counterparts. Likewise, five heterobivalent nanobodies combining Nb4, Nb10, Nb11, Nb36 or Nb37 through [GGGG(S/T)](9) linkers exhibited PAM potencies similar to that of the monovalent version of the first (N-terminal) nanobody in the respective constructs. This prompted us to examine the functional properties of [GGGG(S/T)](9)-linked constructs combining Nb4 and the mGluR5-selective nanobody PAM Nb43(mGlu5). Nb4-L9-Nb43(mGlu5) displayed slightly increased CaSR PAM potency relative to Nb4, whereas Nb43(mGlu5)-L9-Nb4 exhibited ~40- and ~10-fold reduced potency compared with Nb4-L9-Nb43(mGlu5) and Nb4, respectively. The findings indicate that 'tail-to-head' conjugation markedly reduces CaSR binding affinity of the second (C-terminal) nanobody and likely explains the lack of synergistic enhancement of PAM potency in the bivalent constructs. Thus, the suitability of tail-to-head linker conjugation of nanobodies is context dependent, governed by how the nanobody engages its target and by whether the linker perturbs these interactions. CI - (c) 2026 The Author(s). Basic & Clinical Pharmacology & Toxicology published by John Wiley & Sons Ltd on behalf of Nordic Association for the Publication of BCPT (former Nordic Pharmacological Society). FAU - Nabipour, Mahdis AU - Nabipour M AUID- ORCID: 0009-0004-8776-6427 AD - Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. FAU - Skadhauge, Charlotte Hagen AU - Skadhauge CH AD - Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. FAU - Rasmussen, Line Juel AU - Rasmussen LJ AD - Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. FAU - Brauner-Osborne, Hans AU - Brauner-Osborne H AUID- ORCID: 0000-0001-9495-7388 AD - Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. FAU - Mathiesen, Jesper M AU - Mathiesen JM AD - Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. FAU - Jensen, Anders A AU - Jensen AA AUID- ORCID: 0000-0002-7927-5052 AD - Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. LA - eng GR - R346-2020-1829/Lundbeck Foundation/ GR - NNF21OC0069896/Novo Nordisk Fonden/ GR - 1030-00050A/Danmarks Frie Forskningsfond/ PT - Journal Article PL - England TA - Basic Clin Pharmacol Toxicol JT - Basic & clinical pharmacology & toxicology JID - 101208422 RN - 0 (Receptors, Calcium-Sensing) RN - 0 (Single-Domain Antibodies) RN - SY7Q814VUP (Calcium) RN - 0 (Peptides) SB - IM MH - *Receptors, Calcium-Sensing/metabolism MH - Humans MH - HEK293 Cells MH - *Single-Domain Antibodies/pharmacology/chemistry MH - Allosteric Regulation/drug effects MH - Calcium/metabolism MH - *Peptides/chemistry/pharmacology PMC - PMC13716545 OAB - Nanobodies are small camelid antibody fragments that selectively bind their targets and sometimes also alter their function. We have previously identified monovalent nanobodies that potentiate calcium-sensing receptor (CaSR) signalling. Here, we linked different nanobody pairs via peptide linkers to create bivalent constructs and tested their modulatory properties at CaSR. None of the bivalent constructs were more potent CaSR potentiators than the monovalent nanobodies. Further analysis showed that linker-based 'tail-to-head' fusion of the nanobodies greatly reduces the second nanobody's ability to bind CaSR, likely explaining the lack of improved modulator activity. Thus, the usefulness of peptide-linked bivalent nanobodies depends on how each nanobody engages its target. OABL- eng OTO - NOTNLM OT - bivalent nanobodies OT - calcium-sensing receptor (CaSR) OT - nanobodies OT - peptide-linker OT - positive allosteric modulator (PAM) COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 PMCR- 2026/10/29 CRDT- 2026/10/28 21:33 PHST- 2026/09/24 00:00 [revised] PHST- 2026/06/25 00:00 [received] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:33 [entrez] PHST- 2026/10/29 00:00 [pmc-release] AID - e70320 [pii] AID - BCPT70320 [pii] AID - 10.1111/bcpt.70320 [doi] PST - ppublish SO - Basic Clin Pharmacol Toxicol. 2026 Dec;139(6):e70320. doi: 10.1111/bcpt.70320. PMID- 42951370 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1525-6049 (Electronic) IS - 0886-022X (Print) IS - 0886-022X (Linking) VI - 48 IP - 1 DP - 2026 Dec TI - Predicting 12- and 24-month documented CKD progression from routine laboratory data: a single-center temporal evaluation. PG - 2753249 LID - 10.1080/0886022X.2026.2753249 [doi] LID - 2753249 AB - BACKGROUND: Routine laboratory data may support CKD follow-up. We evaluated 12- and 24-month documented progression prediction and exploratory review outputs. METHODS: This retrospective single-center study used 2012-2025 hospital records. CKD required objective persistence for >/=90 days. Development used 2012-2021 indices; temporal evaluation used unseen patients indexed in 2022-2023. Among horizon-evaluable indices, the endpoint was documented sustained >/=40% estimated glomerular filtration rate (eGFR) decline or kidney failure. Development selected 12-month and conditional second-year models combined for non-decreasing cumulative risk. Laboratory and documentation outputs were assessed separately. RESULTS: Temporal evaluation of horizon-evaluable patients included 143 patients (10 event patients; 21/364 positive indices) at 12 months and 87 patients (22 event patients; 50/247 positive indices) at 24 months. Area under the precision-recall curve (AUPRC) was 0.510 (95% confidence interval [CI] 0.217-0.789) and 0.677 (0.459-0.853), respectively. Incremental 12-month AUPRC over age, sex and current eGFR was uncertain (difference -0.009, 95% CI -0.171 to 0.123). At 24 months, area under the receiver operating characteristic curve (AUROC) was higher than the core comparator (difference 0.057, 95% CI 0.007-0.121); clinical utility was not evaluated. Calibration slopes were 0.996 (0.689-1.640) and 1.028 (0.676-1.661); wide CIs precluded reliable individual-risk calibration. Documentation ranking did not improve on prior-mention strategies. CONCLUSIONS: This study is proof of concept. Small event counts, follow-up selection and uncertain incremental value limit interpretation. Multisite validation is required before implementation. FAU - Jin, Mudan AU - Jin M AD - Department of Laboratory Medicine, Shanghai Fengxian District Traditional Chinese Medicine Hospital, Shanghai, China. FAU - Jing, Jie AU - Jing J AD - Department of Laboratory and Pathology, 72nd Group Army Hospital, Huzhou, China. FAU - Lin, Di AU - Lin D AD - Department of Laboratory Medicine, Wangjiang Subdistrict Community Healthcare Center, Shangcheng District, Hangzhou, China. FAU - Li, Jing AU - Li J AD - Department of Laboratory and Pathology, 72nd Group Army Hospital, Huzhou, China. FAU - Zhang, Lang AU - Zhang L AD - Department of Laboratory and Pathology, 72nd Group Army Hospital, Huzhou, China. FAU - Guo, Pinhao AU - Guo P AD - Department of Anesthesiology, 72nd Group Army Hospital, Huzhou Normal University, Huzhou, China. FAU - Wang, Zheng AU - Wang Z AD - Department of Hepatobiliary and Pancreatic Surgery, The Second People's Hospital of Huai'an, the Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, China. FAU - Wen, Xingzhu AU - Wen X AD - Department of General Surgery, 72nd Group Army Hospital, Huzhou Normal University, Huzhou, China. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Ren Fail JT - Renal failure JID - 8701128 SB - IM MH - Humans MH - Retrospective Studies MH - Female MH - Glomerular Filtration Rate MH - Disease Progression MH - *Renal Insufficiency, Chronic/physiopathology/diagnosis MH - Male MH - Aged MH - Middle Aged MH - Time Factors PMC - PMC13717766 OTO - NOTNLM OT - Chronic kidney disease OT - calibration OT - documentation review OT - risk prediction OT - routine laboratory data OT - temporal evaluation COIS- All authors declare no conflicts of interest related to this work. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 PMCR- 2026/10/28 CRDT- 2026/10/28 21:33 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:33 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 2753249 [pii] AID - 10.1080/0886022X.2026.2753249 [doi] PST - ppublish SO - Ren Fail. 2026 Dec;48(1):2753249. doi: 10.1080/0886022X.2026.2753249. Epub 2026 Oct 28. PMID- 42951337 OWN - NLM STAT- Publisher LR - 20261028 IS - 1741-3850 (Electronic) IS - 1741-3842 (Linking) DP - 2026 Oct 28 TI - Unpacking the coastal health gap: the tide is high and so are the disparities in healthy life expectancy in England and Wales. LID - fdag079 [pii] LID - 10.1093/pubmed/fdag079 [doi] AB - BACKGROUND: Coastal communities in England and Wales face unique challenges that may contribute to disparities in healthy life expectancy (HLE). METHODS: This study uses 2021 Census data and mortality records to estimate HLE at birth across 7264 Middle-Layer Super Output Areas (MSOAs) in England and Wales. HLE was calculated using the Sullivan method. Coastal MSOAs were identified using population-weighted centroids within 2 km of the UK coastline. Linear regression models assessed the association between coastal classification and HLE, adjusting for region, deprivation, educational attainment, and industry of employment. Interaction terms explored whether deprivation moderated coastal health disparities. RESULTS: Coastal MSOAs exhibited significantly lower HLE than inland MSOAs, particularly in the South East and East of England regions. After adjusting for socioeconomic factors, the coastal disadvantage was attenuated but remained significant in the most deprived areas of select regions. Area deprivation was a strong predictor of reduced HLE, with each decile increase associated with a 1.5-year reduction in HLE. CONCLUSIONS: The findings challenge the notion of a uniform coastal health penalty, highlighting the importance of regional context and deprivation in shaping health outcomes. Targeted public health interventions should prioritize deprived coastal communities through fairer resource allocation and decentralization of decision-making. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Faculty of Public Health. FAU - Manjon, Adriana AU - Manjon A AD - UCL Institute of Epidemiology and Health Care, Gower Street, WC1E 6BT, London, UK. FAU - Jivraj, Stephen AU - Jivraj S AUID- ORCID: 0000-0001-5751-983X AD - UCL Institute of Epidemiology and Health Care, Gower Street, WC1E 6BT, London, UK. LA - eng GR - ES/X001202/1/Economic and Social Research Council/ PT - Journal Article DEP - 20261028 PL - England TA - J Public Health (Oxf) JT - Journal of public health (Oxford, England) JID - 101188638 SB - IM OTO - NOTNLM OT - communities OT - geography OT - public health EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 21:03 PHST- 2025/12/24 00:00 [received] PHST- 2026/06/05 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:03 [entrez] AID - 9004146 [pii] AID - 10.1093/pubmed/fdag079 [doi] PST - aheadofprint SO - J Public Health (Oxf). 2026 Oct 28:fdag079. doi: 10.1093/pubmed/fdag079. PMID- 42951334 OWN - NLM STAT- Publisher LR - 20261028 IS - 1476-4687 (Electronic) IS - 0028-0836 (Linking) DP - 2026 Oct 28 TI - Switchable Chlorine Atom Transfer Radical Reactions. LID - 10.1038/s41586-026-11348-z [doi] AB - Atom transfer radical reactions-encompassing atom transfer radical addition (ATRA)(1) and atom transfer radical polymerization (ATRP)(2)-provide a versatile route to synthesize functionalized small molecules and polymers. Using readily available unactivated alkyl chlorides(3) instead of activated organohalides represents the state-of-the-art in this field, as this requires cleaving stronger C-Cl bonds in the substrates while preserving weaker ones in the products. Here we describe a dual-copper relay catalytic strategy to overcome this limitation. A dinuclear copper complex selectively activates the robust C-Cl bond of unactivated alkyl chlorides, while a mononuclear copper catalyst mediates controllable C-Cl bond reconstruction. The reaction pathway can be switched between ATRA and ATRP-type reactions in the presence or absence of the mononuclear copper partner, thus providing a switchable platform for chlorine atom transfer radical reactions. This protocol can not only enable synthesis of structurally diverse ATRA products and alpha-end-functionalized polyacrylates, but allow for direct modification of poly(vinyl chloride) (PVC) under mild reaction conditions. It demonstrates the successful utility of unactivated organochlorides in organic synthesis and polymer science. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature Limited. FAU - Huang, Jun AU - Huang J AD - State Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China. FAU - Liu, Yu AU - Liu Y AD - State Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China. FAU - Yan, Wencheng AU - Yan W AD - State Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China. FAU - Dong, Guichao AU - Dong G AD - State Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China. FAU - Zhang, Xinyue AU - Zhang X AD - School of Pharmacy, Xuzhou Medical University, Xuzhou, China. FAU - Chen, Hongliang AU - Chen H AD - State Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China. FAU - Zhao, Shiyi AU - Zhao S AD - State Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China. FAU - Xu, Zhou AU - Xu Z AD - School of Pharmacy, Xuzhou Medical University, Xuzhou, China. FAU - Zhu, Chengjian AU - Zhu C AUID- ORCID: 0000-0003-4465-9408 AD - State Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China. FAU - Han, Jie AU - Han J AUID- ORCID: 0009-0006-1076-1454 AD - State Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China. FAU - Xie, Jin AU - Xie J AUID- ORCID: 0000-0003-2600-6139 AD - State Key Laboratory of Coordination Chemistry, Jiangsu Key Laboratory of Advanced Organic Materials, Chemistry and Biomedicine Innovation Center (ChemBIC), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, China. xie@nju.edu.cn. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Nature JT - Nature JID - 0410462 SB - IM EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 21:03 PHST- 2025/07/23 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:03 [entrez] AID - 10.1038/s41586-026-11348-z [pii] AID - 10.1038/s41586-026-11348-z [doi] PST - aheadofprint SO - Nature. 2026 Oct 28. doi: 10.1038/s41586-026-11348-z. PMID- 42951332 OWN - NLM STAT- Publisher LR - 20261028 IS - 1445-2197 (Electronic) IS - 1445-1433 (Linking) DP - 2026 Oct 28 TI - Optimising Paediatric Gastrostomy Outcomes Through Appropriate Procedural Selection: A 10-Year Retrospective Cohort Study. LID - 10.1111/ans.71019 [doi] AB - BACKGROUND: Gastrostomy is a common intervention for enteral nutrition in children. While originally an open procedure, techniques now include percutaneous endoscopic (PEG), laparoscopic (LAP) and laparoscopic-assisted PEG (LAPEG). This study compared outcomes across these four techniques over a 10-year period at a single tertiary paediatric centre. METHODS: A retrospective cohort study was conducted on all initial gastrostomies performed between January 2014 and September 2024. Data collected included patient demographics, clinical characteristics and procedural details. Primary outcomes were intra and postoperative complications. Secondary outcomes included procedure time, length of stay and gastrostomy-related readmission within 1 year. Statistical analysis utilised chi-squared, Fisher's exact tests and Kruskal-Wallis test. RESULTS: A total of 431 patients underwent gastrostomy (PEG = 318, LAPEG = 53, LAP = 36, Open = 24). PEG demonstrated the shortest procedure time, while Open gastrostomy was significantly longer (p < 0.05). Intraoperative complications were rare (2.3%) and did not differ significantly between techniques. Post-operative complication rates differed significantly (p = 0.036), being lowest in LAPEG (24.5%) and highest in Open (58.3%). Gastrostomy related postoperative complications did not differ significantly between groups (p = 0.112). CONCLUSION: PEG demonstrated increased efficiency for appropriately selected patients, while LAPEG and LAP were valuable options for cases requiring additional operative flexibility. Open gastrostomy retains an important role in patients whose complexity precludes minimally invasive methods. The comparable safety across techniques reflects the multidisciplinary approach to matching procedural complexity with patient needs, highlighting case selection as the key determinant of clinical outcomes. CI - (c) 2026 The Author(s). ANZ Journal of Surgery published by John Wiley & Sons Australia, Ltd on behalf of Royal Australasian College of Surgeons. FAU - Sharma, Ashvin AU - Sharma A AUID- ORCID: 0009-0006-6202-8334 AD - Faculty of Medicine, Dentistry and Health Sciences, The University of Western Australia, Crawley, Western Australia, Australia. FAU - Shah, Syed Saad Ali AU - Shah SSA AUID- ORCID: 0009-0009-4530-7544 AD - Faculty of Medicine, Dentistry and Health Sciences, The University of Western Australia, Crawley, Western Australia, Australia. FAU - Fernando, Dhanushke T AU - Fernando DT AUID- ORCID: 0000-0002-3274-9353 AD - Department of Paediatric Surgery, Perth Children's Hospital, Nedlands, Western Australia, Australia. FAU - Das, Adarsh AU - Das A AUID- ORCID: 0000-0001-6289-4319 AD - Palliative Care Service, Sir Charles Gairdner Hospital, Nedlands, Western Australia, Australia. AD - School of Public Health, Faculty of Health, Medicine and Behavioural Sciences, University of Queensland, Brisbane, Queensland, Australia. FAU - Gera, Parshotam AU - Gera P AD - Department of Paediatric Surgery, Perth Children's Hospital, Nedlands, Western Australia, Australia. AD - Curtin Medical School, Faculty of Health Sciences, Curtin University, Perth, Western Australia, Australia. LA - eng PT - Journal Article DEP - 20261028 PL - Australia TA - ANZ J Surg JT - ANZ journal of surgery JID - 101086634 SB - IM OTO - NOTNLM OT - LAP OT - LAPEG OT - PEG OT - gastrostomy OT - paediatric EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 21:03 PHST- 2026/10/05 00:00 [revised] PHST- 2026/07/03 00:00 [received] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 21:03 [entrez] AID - 10.1111/ans.71019 [doi] PST - aheadofprint SO - ANZ J Surg. 2026 Oct 28. doi: 10.1111/ans.71019. PMID- 42951305 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1939-0041 (Electronic) IS - 1939-0041 (Linking) VI - 18 IP - 6 DP - 2026 Nov-Dec TI - Nanozyme-Driven Next-Generation miRNA Biosensing. LID - e70083 [pii] LID - 10.1002/wnan.70083 [doi] AB - MicroRNAs (miRNAs) are small, noncoding RNA molecules nearly 22 nucleotides in length that contribute significantly to regulating gene expression. Their aberrant expression patterns are associated with disease progression, including cardiovascular and neurological disorders and cancer. However, the rapid detection of miRNAs remains challenging due to their low abundance, sequence similarity, and degradation. Conventional methods of detecting miRNAs, such as Northern blotting, microarray analysis, quantitative reverse transcription polymerase chain reaction (RT-qPCR), and next-generation sequencing (NGS), offer significant benefits for miRNA profiling, though they have drawbacks, including limited specificity, complex workflows, relatively high cost, and specialized equipment requirements. Advanced methodologies driven by nanotechnology, especially those that combine clustered regularly interspaced short palindromic repeats (CRISPR)/Cas approaches, provide revolutionary developments. Nanozymes enhance the stability and biocompatibility of catalytic amplification, while CRISPR technologies enable real-time, multiplex, and ultrasensitive miRNA detection with accurate sequence recognition. The rapidly expanding field encompasses diverse nanozyme architectures, recognition mechanisms, amplification strategies, and signal-transduction platforms, providing a broad basis for critically assessing advances, limitations, and emerging opportunities in nanozyme-enabled miRNA biosensing. Nanozyme-enabled miRNA detection has evolved across diverse sensing platforms, such as colorimetric and optical assays, sophisticated photoelectrochemical (PEC) and electrochemiluminescence (ECL) biosensors, and portable and paper-based diagnostic technologies. The convergence of nanozyme catalysis, CRISPR-based recognition, and advanced sensing strategies holds promise for sensitive, selective, and portable miRNA diagnostics. CI - (c) 2026 Wiley Periodicals LLC. FAU - Deshwal, Akanksha AU - Deshwal A AUID- ORCID: 0000-0001-5581-0383 AD - Amity Institute of Nanotechnology, Amity University Uttar Pradesh (AUUP), Noida, India. FAU - Tripathi, Ravi Mani AU - Tripathi RM AUID- ORCID: 0000-0001-9417-4615 AD - Amity Institute of Nanotechnology, Amity University Uttar Pradesh (AUUP), Noida, India. LA - eng GR - TAR/2022/000074/Anusandhan National Research Foundation (ANRF)/ PT - Journal Article PT - Review PL - United States TA - Wiley Interdiscip Rev Nanomed Nanobiotechnol JT - Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology JID - 101508311 RN - 0 (MicroRNAs) SB - IM MH - *MicroRNAs/analysis MH - *Biosensing Techniques MH - Humans MH - Animals MH - *Nanostructures/chemistry MH - *Nanotechnology OTO - NOTNLM OT - CRISPR-Cas OT - microRNA OT - nanozymes OT - paper-based biosensor EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 20:53 PHST- 2026/10/05 00:00 [revised] PHST- 2025/10/30 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:53 [entrez] AID - e70083 [pii] AID - 10.1002/wnan.70083 [doi] PST - ppublish SO - Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2026 Nov-Dec;18(6):e70083. doi: 10.1002/wnan.70083. PMID- 42951302 OWN - NLM STAT- Publisher LR - 20261028 IS - 1538-8654 (Electronic) IS - 1527-7941 (Linking) DP - 2026 Oct 28 TI - Stoma Disgust in Colorectal Cancer Patients With Enterostomy: A Literature Review. LID - 10.1097/ASW.0000000000000542 [doi] AB - OBJECTIVE: To discuss the concept, assessment tools, influencing factors, and intervention measures of stoma disgust. DATA SOURCES: Publications on stoma disgust in colorectal cancer patients with enterostomy were retrieved, summarized, and evaluated by a team of investigators and clinical experts. STUDY SELECTION: Full-text articles written in English and the Chinese language, and studying stoma disgust in colorectal cancer patients with enterostomy. DATA EXTRACTION: The research topic includes the concept, assessment tools, influencing factors, and intervention measures of stoma disgust. DATA SYNTHESIS: This study reviews the concept, assessment tools, influencing factors, and intervention measures of stoma disgust. The level of disgust in stoma patients is influenced by gender, age, stoma type, preoperative education, length of hospital stay, self-efficacy, social support, and cultural and religious beliefs. Psychotherapy, improving patient self-efficacy, and social support can effectively reduce stoma disgust levels. CONCLUSIONS: Stoma disgust is a common emotional problem faced by colorectal cancer patients with an enterostomy. Future research could develop comprehensive measurement tools for stoma disgust, and future qualitative research could delve deeper into the influencing factors of stoma disgust and implement targeted, multidimensional clinical interventions to reduce stoma disgust. CI - Copyright (c) 2026 the Author(s). Published by Wolters Kluwer Health, LLC. FAU - Luo, Jiandong AU - Luo J AD - Department of Endoscopy Center, Hangzhou Red Cross Hospital, Hangzhou, Zhejiang, China. FAU - Wu, Dengzhu AU - Wu D AD - Department of Endoscopy Center, Hangzhou Red Cross Hospital, Hangzhou, Zhejiang, China. FAU - Tan, Guilin AU - Tan G AD - Department of Endoscopy Center, Hangzhou Red Cross Hospital, Hangzhou, Zhejiang, China. FAU - Fang, Bifei AU - Fang B AD - Department of Endoscopy Center, Hangzhou Red Cross Hospital, Hangzhou, Zhejiang, China. FAU - Hu, Jiajie AU - Hu J AUID- ORCID: 0009-0005-3860-7654 AD - Nursing Department, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China. LA - eng GR - ZHKYQ202215/Project of 2022 Annual Scientific Research Project of the Chinese Nursing Association/ PT - Journal Article DEP - 20261028 PL - United States TA - Adv Skin Wound Care JT - Advances in skin & wound care JID - 100911021 SB - IM OTO - NOTNLM OT - colorectal cancer OT - enterostomy OT - research progress OT - stoma disgust EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:43 PHST- 2025/12/22 00:00 [received] PHST- 2026/07/23 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:43 [entrez] AID - 00129334-990000000-00246 [pii] AID - 10.1097/ASW.0000000000000542 [doi] PST - aheadofprint SO - Adv Skin Wound Care. 2026 Oct 28. doi: 10.1097/ASW.0000000000000542. PMID- 42951287 OWN - NLM STAT- Publisher LR - 20261028 IS - 1531-4995 (Electronic) IS - 0023-852X (Linking) DP - 2026 Oct 28 TI - Auditory Temporal Processing in Patients With Vestibular Migraine. LID - 10.1002/lary.71030 [doi] AB - OBJECTIVE: Vestibular migraine (VM) has been associated with central sensory processing abnormalities and neural hyperexcitability, which may disrupt temporal coding despite normal peripheral hearing. This study aimed to determine whether auditory temporal processing is differentially affected in VM by comparing temporal resolution and temporal masking abilities with those of healthy controls. METHODS: This cross-sectional study included 17 adults aged 20-50 years with definite VM and 17 age- and sex-matched healthy controls. All participants had normal hearing sensitivity and normal cognitive function, confirmed using the Standardized Mini-Mental State Examination (SMMSE). Auditory temporal processing was evaluated using the Gap Detection Test (GDT) and the Forward Masking Test (FMT). Group differences were analyzed using appropriate statistical methods. RESULTS: No significant differences were observed between the groups regarding age or sex. Participants with VM demonstrated significantly poorer GDT performance compared with healthy controls, indicating impaired auditory temporal resolution (p < 0.05). In contrast, no significant difference was found between the groups in FMT performance (p > 0.05). CONCLUSIONS: Individuals with VM exhibit selective impairment of auditory temporal processing despite normal peripheral hearing. These findings support the inclusion of central auditory temporal processing assessment as part of the comprehensive audiological evaluation of this population and suggest that auditory training targeting temporal processing deficits may represent a potential avenue for future rehabilitation. CI - (c) 2026 The American Laryngological, Rhinological and Otological Society, Inc. FAU - Bozkurt, Hatice Kubra AU - Bozkurt HK AUID- ORCID: 0000-0003-3189-058X AD - Department of Audiology, Gulhane Faculty of Health Sciences, University of Health Sciences, Ankara, Turkiye. AD - Department of Otorhinolaryngology, University of Health Sciences, Gulhane Training and Research Hospital, Ankara, Turkey. FAU - Akin Ocal, Fatma Ceyda AU - Akin Ocal FC AUID- ORCID: 0000-0001-7212-2208 AD - Department of Otorhinolaryngology, University of Health Sciences, Gulhane Training and Research Hospital, Ankara, Turkey. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Laryngoscope JT - The Laryngoscope JID - 8607378 SB - IM OTO - NOTNLM OT - forward masking OT - gap detection OT - temporal masking OT - temporal resolution OT - vestibular migraine EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:43 PHST- 2026/10/02 00:00 [revised] PHST- 2026/07/26 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:43 [entrez] AID - 10.1002/lary.71030 [doi] PST - aheadofprint SO - Laryngoscope. 2026 Oct 28. doi: 10.1002/lary.71030. PMID- 42951266 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-3150 (Electronic) IS - 0041-0101 (Linking) DP - 2026 Oct 28 TI - Proteomic and Functional Comparison of Active-Season and Overwintering-Stage Venoms from Naja atra. PG - 109342 LID - S0041-0101(26)00360-0 [pii] LID - 10.1016/j.toxicon.2026.109342 [doi] AB - Seasonal changes in physiological state and metabolism may influence snake venom composition and biological activity, yet these relationships remain poorly understood. This study investigated seasonal variation in venom composition and functional properties in Naja atra. Venom samples were collected from the same ten adult snakes during the active season and after approximately two months of overwintering. Venom yield, protein composition, and multiple biological activities were compared using quantitative proteomic profiling and functional assays. Proteomic quantification was based on scaled protein abundance values exported from Proteome Discoverer (version 3.3), with peptide and protein identification performed using the Sequest HT search engine. Overwintering was accompanied by body-weight loss and reductions in venom yield, with wet and dry venom yields decreasing by 37.75% and 30.06%, respectively. Proteomic analysis identified 178 protein entries in active-season venom (AV) and 163 in overwintering venom (HV), with 142 shared entries, indicating substantial conservation of the detectable venom protein repertoire alongside compositional changes. Three-finger toxins showed lower relative abundance during overwintering, whereas snake venom metalloproteinases and L-amino acid oxidase exhibited relatively higher representation. Functional assays revealed increased phospholipase A(2) (PLA(2)) activity and decreased proteolytic activity in overwintering venom, whereas L-amino acid oxidase activity, nicotinic acetylcholine receptor (nAChR)-binding activity, cytotoxicity, and hemolytic activity remained largely comparable between groups. These findings indicate that overwintering is associated with reduced venom yield and selective remodeling of venom composition and function rather than uniform suppression of venom activity. Although the underlying mechanisms remain unresolved, this study highlights physiological state as an important consideration in understanding snake venom plasticity. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Hu, Jianguo AU - Hu J AD - Faculty of Modern Health Care, Anhui Sanlian University, Hefei, Anhui, China. FAU - Lu, Xin AU - Lu X AD - Faculty of Modern Health Care, Anhui Sanlian University, Hefei, Anhui, China. FAU - Guo, Hongxia AU - Guo H AD - Faculty of Modern Health Care, Anhui Sanlian University, Hefei, Anhui, China. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Toxicon JT - Toxicon : official journal of the International Society on Toxinology JID - 1307333 SB - IM OTO - NOTNLM OT - 3FTx OT - Naja atra OT - PLA(2) OT - overwintering OT - venom COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:37 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:37 [entrez] AID - S0041-0101(26)00360-0 [pii] AID - 10.1016/j.toxicon.2026.109342 [doi] PST - aheadofprint SO - Toxicon. 2026 Oct 28:109342. doi: 10.1016/j.toxicon.2026.109342. PMID- 42951258 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-4995 (Electronic) IS - 0168-3659 (Linking) DP - 2026 Oct 28 TI - Tetrahedral DNA engineered Janus nanoplatform for potentiated Zn(2+) interference therapy and cGAS-STING activation via efficient delivery of exogenous DNA. PG - 115464 LID - S0168-3659(26)00868-0 [pii] LID - 10.1016/j.jconrel.2026.115464 [doi] AB - While zinc-ion interference therapy presents a compelling approach for cancer immunotherapy, poor intracellular bioavailability and weak innate immune activation restrict its therapeutic potential. To overcome these bottlenecks, we developed tetrahedral DNA-integrated ZnFe(2)O(4)-ZnO Janus nanoparticles (TDN-ZIZJP) to achieve spatiotemporal modulation of immunity. Distinct from conventional cubic nanoparticles, this nail-shaped Janus architecture amplifies cell-particle interactions, substantially improving tumor penetration and cellular uptake. This unique feature endows improved selectively dissolving of ZnO domain within the mildly acidic tumor microenvironment, resulting in a targeted intracellular surge of Zn(2+). This enhanced ion flux provokes oxidative stress and endogenous DNA damage, thereby increasing the availability of endogenous cytosolic DNA. Moreover, the integrated tetrahedral DNA nanostructures serve as a robust reservoir, delivering abundant exogenous dsDNA directly into the cytoplasm. Zn(2+)-induced endogenous DNA release and TDN-derived exogenous dsDNA jointly increase the availability of cytosolic DNA substrates for cGAS. This dual-source DNA supply supports sustained cGAS-STING activation, thereby enhancing type I interferon signaling, promoting dendritic cell maturation and increasing cytotoxic T-cell infiltration. This study provides a new avenue that couples Zn(2+)-mediated ion interference and endogenous DNA damage with an exogenous dsDNA reservoir, providing a highly tunable platform for metal ion-regulated immunotherapy. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Tan, Mingya AU - Tan M AD - College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China. FAU - Liu, Meiling AU - Liu M AD - College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China; Department of Radiology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China. FAU - Sun, Xin AU - Sun X AD - 96604 Military Hospital, Lanzhou 730000, China. FAU - Li, Jiangyang AU - Li J AD - Cancer Center, Daping Hospital, Army Medical University, Chongqing 400042, China. FAU - Huo, Linlin AU - Huo L AD - College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China; Department of Respiratory and Critical Care Medicine, the Second Affiliated Hospital of Third Military Medical University, Army Medical University, Chongqing 400037, China. FAU - Fan, Mengke AU - Fan M AD - College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China; Department of Radiology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China. FAU - Zhao, Jiayi AU - Zhao J AD - College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China. FAU - Liu, Chunlu AU - Liu C AD - College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China. FAU - Zhao, Zhenghuan AU - Zhao Z AD - College of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China. Electronic address: roddirck@cqmu.edu.cn. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - J Control Release JT - Journal of controlled release : official journal of the Controlled Release Society JID - 8607908 SB - IM OTO - NOTNLM OT - Ion-interference therapy OT - Janus nanoparticles OT - Synergistic cancer therapy OT - Tetrahedral DNA nanostructure OT - cGAS-STING pathway COIS- Declaration of competing interest The authors declare no conflict of interest. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:37 PHST- 2026/07/06 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:37 [entrez] AID - S0168-3659(26)00868-0 [pii] AID - 10.1016/j.jconrel.2026.115464 [doi] PST - aheadofprint SO - J Control Release. 2026 Oct 28:115464. doi: 10.1016/j.jconrel.2026.115464. PMID- 42951257 OWN - NLM STAT- Publisher LR - 20261028 IS - 1872-7697 (Electronic) IS - 0167-8760 (Linking) DP - 2026 Oct 28 TI - Neural, vascular, motor and autonomic habituation to high-intensity auditory stimulation: A multimodal characterization of the human defensive responses. PG - 113501 LID - S0167-8760(26)00184-4 [pii] LID - 10.1016/j.ijpsycho.2026.113501 [doi] AB - The defense reflex has been extensively characterized across sensory modalities; however, the integration of electrical, neurovascular, motor, and peripheral autonomic responses involving the central and autonomic nervous systems remains poorly understood. This study investigated defensive responses to high-intensity auditory stimulation using a multimodal approach combining electroencephalography (EEG), functional near-infrared spectroscopy (fNIRS), and peripheral physiological measures. Participants were exposed to twenty white-noise bursts (100 dB, 500 ms, instantaneous rise time) separated by silent intervals (50 +/- 10 s), grouped into five blocks to assess initial responses and habituation. Hemodynamic responses revealed widespread activation of bilateral auditory and frontal regions following stimulus onset, with a progressive reduction in response amplitude and extension of active channels across blocks. Network analysis suggests progressive reorganization of functional connectivity between sensory and frontal regions across repeated stimulation. At the peripheral level, habituation was observed in phasic electrodermal activity, vascular indices, motor response, EEG-derived ocular activity, and parasympathetic cardiac modulation, primarily after the first block, whereas respiratory measures remained stable. Cardiac defense-like responses show a multi-temporal component structure, with habituation restricted to the late decelerative phase. Multivariate analyses revealed a structured yet partially independent organization of neural and autonomic responses to habituation. Together, these findings provide a comprehensive characterization of multisystem responses to intense auditory stimulation, highlighting selective habituation across central, neurovascular, motor, and autonomic systems, and offering new insights into how neurovascular and peripheral processes adapt to repeated auditory threats. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Munoz, Vanesa AU - Munoz V AD - Human Psychobiology Laboratory, Experimental Psychology Department, University of Seville, Spain. Electronic address: lmunnoz@us.es. FAU - Angulo-Ruiz, Brenda Y AU - Angulo-Ruiz BY AD - Human Psychobiology Laboratory, Experimental Psychology Department, University of Seville, Spain. Electronic address: bangulo@us.es. FAU - Gomez, Carlos M AU - Gomez CM AD - Human Psychobiology Laboratory, Experimental Psychology Department, University of Seville, Spain. Electronic address: cgomez@us.es. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Int J Psychophysiol JT - International journal of psychophysiology : official journal of the International Organization of Psychophysiology JID - 8406214 SB - IM OTO - NOTNLM OT - Defensive responses OT - Habituation OT - High-auditory stimulation OT - Multimodal recordings OT - Peripheral responses OT - Startle EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:37 PHST- 2026/06/29 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:37 [entrez] AID - S0167-8760(26)00184-4 [pii] AID - 10.1016/j.ijpsycho.2026.113501 [doi] PST - aheadofprint SO - Int J Psychophysiol. 2026 Oct 28:113501. doi: 10.1016/j.ijpsycho.2026.113501. PMID- 42951252 OWN - NLM STAT- Publisher LR - 20261028 IS - 1530-0285 (Electronic) IS - 0893-3952 (Linking) DP - 2026 Oct 28 TI - Invasive Lobular Carcinoma With Extracellular Mucin Production: First Whole Exome Sequencing of a Rare Histological Variant of Breast Carcinoma. PG - 101110 LID - S0893-3952(26)00153-5 [pii] LID - 10.1016/j.modpat.2026.101110 [doi] AB - Invasive lobular carcinoma with extracellular mucin production (ILCEMP) is a rare histological variant of invasive lobular carcinoma (ILC), with only about 40 cases described in the English literature. In the latest WHO classification of breast tumours, ILCEMP is described under the mucinous carcinoma section, noting that it is not certain whether these tumours represent a subtype of ILC or mucinous carcinoma. We performed whole-exome sequencing in seven ILCEMP primary tumors and two matched regional metastatic lymph nodes from seven patients. Samples were provided by six different European institutions. Most cases have been previously described histologically in two studies. Genomically, apart from 16q deletions, recurrent deletions were identified in 8p (3/7 patients), 11p (3/7 patients), 11q (5/7 patients), 17p (3/7 patients), 18p (6/7 patients), 18q (4/7 patients), 19p (3/7 patients) and Xq (6/7 patients). Concerning somatic mutations, the majority were missense mutations, followed by frameshift deletions, splice-site mutations, and nonsense mutations. CDH1 alterations were detected in six tumors, coupled with CDH1 LOH or 16q loss in five of them. Copy number variations were found with SNV/Indel alterations in AXIN2, CTNNA1, CTNNA2, and CTNNB1 (5/7 cases). TP53 was targeted in four patients. Also, ERBB2 carried SNVs and CNVs (>4 CN) in 4 patients. Alterations affecting the RTK-RAS signaling axis, an ANK1-associated cytoskeletal module, and genes involved in chromatin remodeling, including KMT2C, were also identified. Overall, our findings support the notion that ILCEMP displays a lobular rather than mucinous molecular profile and reveal potentially targetable molecular alterations. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Koufopoulos, Nektarios I AU - Koufopoulos NI AD - 2nd Department of Pathology, Medical School, National and Kapodistrian University of Athens, "Attikon" University Hospital, Athens, Greece. Electronic address: nkoufo@med.uoa.gr. FAU - Tsampazis, Georgios Kousis AU - Tsampazis GK AD - Department of Biology, School of Sciences and Engineering, University of Crete, Voutes University Campus, Heraklion, Greece; Institute of Computer Science, Foundation for Research and Technology - Hellas (FORTH), Heraklion, Greece. FAU - Pateras, Ioannis S AU - Pateras IS AD - 2nd Department of Pathology, Medical School, National and Kapodistrian University of Athens, "Attikon" University Hospital, Athens, Greece. FAU - Spathis, Aris AU - Spathis A AD - 2nd Department of Pathology, Medical School, National and Kapodistrian University of Athens, "Attikon" University Hospital, Athens, Greece. FAU - Zacharioudakis, Methodios AU - Zacharioudakis M AD - Department of Biology, School of Sciences and Engineering, University of Crete, Voutes University Campus, Heraklion, Greece. FAU - Leventakou, Danai AU - Leventakou D AD - 2nd Department of Pathology, Medical School, National and Kapodistrian University of Athens, "Attikon" University Hospital, Athens, Greece. FAU - Samaras, Menelaos G AU - Samaras MG AD - 2nd Department of Pathology, Medical School, National and Kapodistrian University of Athens, "Attikon" University Hospital, Athens, Greece. FAU - De Gaetano, James AU - De Gaetano J AD - Department of Pathology, Mater Dei Hospital, Msida, Malta. FAU - Khaldi, Lubna AU - Khaldi L AD - Department of Pathology, "Saint Savvas" Anticancer Hospital, Athens, Greece. FAU - Stahls, Anders AU - Stahls A AD - HUH Diagnostic Center, University of Helsinki and Helsinki University Hospital, Helsinki, Finland. FAU - Varga, Zsuzsanna AU - Varga Z AD - Department of Pathology and Molecular Pathology, University Hospital Zurich, Zurich, Switzerland. FAU - Psyrri, Amanda AU - Psyrri A AD - Oncology Section, 2nd Department of Internal Medicine Propaedeutic, Medical School, National and Kapodistrian University of Athens, "Attikon" University Hospital, Athens, Greece. FAU - Arkadopoulos, Nikolaos AU - Arkadopoulos N AD - 4th Department of Surgery, Medical School, National and Kapodistrian University of Athens, "Attikon" University Hospital, Athens, Greece. FAU - Foukas, Periklis G AU - Foukas PG AD - 2nd Department of Pathology, Medical School, National and Kapodistrian University of Athens, "Attikon" University Hospital, Athens, Greece. FAU - Pavlidis, Pavlos AU - Pavlidis P AD - Department of Biology, School of Sciences and Engineering, University of Crete, Voutes University Campus, Heraklion, Greece; Institute of Computer Science, Foundation for Research and Technology - Hellas (FORTH), Heraklion, Greece. FAU - Frisan, Teresa AU - Frisan T AD - Department of Molecular Biology, Umea Centre for Microbial Research (UCMR), Umea University, Umea, Sweden. FAU - Cserni, Gabor AU - Cserni G AD - Department of Pathology, Albert Szent-Gyorgyi Faculty of Medicine, University of Szeged, Szeged, Hungary; Department of Pathology, Bacs-Kiskun County Teaching Hospital, Kecskemet, Hungary. FAU - Panayiotides, Ioannis G AU - Panayiotides IG AD - 2nd Department of Pathology, Medical School, National and Kapodistrian University of Athens, "Attikon" University Hospital, Athens, Greece; 1st Department of Pathology, Medical School, National and Kapodistrian University of Athens, Athens, Greece. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Mod Pathol JT - Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc JID - 8806605 SB - IM OTO - NOTNLM OT - ANK1 OT - CDH1 OT - KRAS OT - chromatin remodelers OT - invasive lobular carcinoma with extracellular mucin production OT - whole exome sequencing OT - beta-catenin axis EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:37 PHST- 2026/04/14 00:00 [received] PHST- 2026/09/12 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:37 [entrez] AID - S0893-3952(26)00153-5 [pii] AID - 10.1016/j.modpat.2026.101110 [doi] PST - aheadofprint SO - Mod Pathol. 2026 Oct 28:101110. doi: 10.1016/j.modpat.2026.101110. PMID- 42951248 OWN - NLM STAT- Publisher LR - 20261028 IS - 1876-4754 (Electronic) IS - 1876-4754 (Linking) DP - 2026 Oct 28 TI - Low-intensity pulsed ultrasound activates Piezo1 to restore brain lymphatic drainage and improve outcomes in traumatic brain injury mice. PG - 103245 LID - S1935-861X(26)00222-6 [pii] LID - 10.1016/j.brs.2026.103245 [doi] AB - BACKGROUND: Meningeal lymphatic vessels (mLVs) are specialized lymphatic structures that drain cerebrospinal fluid (CSF) and facilitate immune surveillance of the central nervous system. This drainage system is impaired after traumatic brain injury (TBI); mLVs undergo structural regression, and CSF outflow to peripheral lymphoid tissues is markedly slowed. The resulting accumulation of hyperphosphorylated tau, amyloid-beta, and inflammatory mediators drives sustained neuroinflammation and cognitive decline. The neuroprotective effect of low-intensity pulsed ultrasound (LIPUS) in TBI mice has been previously demonstrated, however, its impact on brain lymphatic drainage is unclear. METHODS: Here, we employed a controlled cortical impact (CCI) mouse model of TBI combined with serial LIPUS stimulation to investigate its therapeutic potential for mLV function. RESULTS: LIPUS restored neurological function in TBI mice, improving motor coordination and spatial/recognition memory while attenuating tau pathology, Abeta burden, and astrocyte reactivity. Mechanistically, LIPUS activated Piezo1 on meningeal lymphatic endothelial cells (LECs), triggering a downstream signaling cascade that promoted LEC proliferation and migration in vitro and restored lymphatic coverage and drainage function in vivo. To identify the critical mediator of this process, we examined the pathway's downstream components and found that vascular endothelial growth factor C (VEGFC) served as the critical downstream effector: VEGFC overexpression rescued meningeal lymphatic restoration and CSF drainage. CONCLUSIONS: These results establish LIPUS as a non-invasive therapeutic strategy that functionally restores brain lymphatic drainage after TBI, representing a promising preclinical therapeutic strategy for proteinopathy-associated neurological diseases that warrants further optimization for clinical translation. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Wang, Shuo AU - Wang S AD - Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing 100003, China; Department of Neurosurgery, Tianjin Neurological Institute, State Key Laboratory of Experimental Hematology, Laboratory of Post-Neuroinjury Neurorepair and Regeneration in Central Nervous System Tianjin & Ministry of Education, Tianjin Medical University General Hospital, Tianjin 300052, China. FAU - Wang, Bo AU - Wang B AD - Department of Neurosurgery, Tianjin Neurological Institute, State Key Laboratory of Experimental Hematology, Laboratory of Post-Neuroinjury Neurorepair and Regeneration in Central Nervous System Tianjin & Ministry of Education, Tianjin Medical University General Hospital, Tianjin 300052, China. FAU - Wang, Yuchang AU - Wang Y AD - Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing 100003, China; Department of Neurosurgery, Tianjin Neurological Institute, State Key Laboratory of Experimental Hematology, Laboratory of Post-Neuroinjury Neurorepair and Regeneration in Central Nervous System Tianjin & Ministry of Education, Tianjin Medical University General Hospital, Tianjin 300052, China. FAU - Nie, Meng AU - Nie M AD - Department of Neurosurgery, Tianjin Neurological Institute, State Key Laboratory of Experimental Hematology, Laboratory of Post-Neuroinjury Neurorepair and Regeneration in Central Nervous System Tianjin & Ministry of Education, Tianjin Medical University General Hospital, Tianjin 300052, China. FAU - Yuan, Jiangyuan AU - Yuan J AD - Department of Neurosurgery, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310000, China. FAU - Wu, Chenrui AU - Wu C AD - Department of Neurosurgery, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 610072, China. FAU - Mi, Liang AU - Mi L AD - Department of Neurosurgery, Tianjin Neurological Institute, State Key Laboratory of Experimental Hematology, Laboratory of Post-Neuroinjury Neurorepair and Regeneration in Central Nervous System Tianjin & Ministry of Education, Tianjin Medical University General Hospital, Tianjin 300052, China. FAU - Chen, Bo AU - Chen B AD - Department of Neurosurgery, Tianjin Neurological Institute, State Key Laboratory of Experimental Hematology, Laboratory of Post-Neuroinjury Neurorepair and Regeneration in Central Nervous System Tianjin & Ministry of Education, Tianjin Medical University General Hospital, Tianjin 300052, China. FAU - Liu, Tao AU - Liu T AD - Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing 100003, China; The George Institute for Global Health, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia. Electronic address: TLiu1@georgeinstitute.org.au. FAU - Jiang, Rongcai AU - Jiang R AD - Department of Neurosurgery, Xuanwu Hospital, Capital Medical University, Beijing 100003, China. Electronic address: jiang116216@163.com. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Brain Stimul JT - Brain stimulation JID - 101465726 SB - IM OTO - NOTNLM OT - Low-intensity pulsed ultrasound OT - Meningeal lymphatic vessels OT - Piezo1 OT - Traumatic brain injury OT - Vascular endothelial growth factor C COIS- Declaration of Competing Interest ☐ The authors declare that they have no competing financial interests or personal relationships that could be perceived to have influenced the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:37 PHST- 2026/07/06 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:37 [entrez] AID - S1935-861X(26)00222-6 [pii] AID - 10.1016/j.brs.2026.103245 [doi] PST - aheadofprint SO - Brain Stimul. 2026 Oct 28:103245. doi: 10.1016/j.brs.2026.103245. PMID- 42951244 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-7064 (Electronic) IS - 0028-3908 (Linking) DP - 2026 Oct 28 TI - SCN2B Drives Depression-like Phenotypes in Mice by Suppressing BDNF Signaling via Its Intracellular Domain NICD. PG - 111242 LID - S0028-3908(26)00417-X [pii] LID - 10.1016/j.neuropharm.2026.111242 [doi] AB - The rising global burden of depression necessitates novel mechanistic insights and therapeutic targets. Voltage-gated sodium channel beta2-subunit (Navbeta2, coded by SCN2B) has been implicated in neuropsychiatric disorders, yet its role in depression remains unexplored. Here, we identify SCN2B as a previously unrecognized driver of depression-like pathophysiology. Using SCN2B-overexpressing (SCN2B-OV) mice across different ages (1, 12, and 20 months) and CRISPR- engineered Navbeta2(V173A,V175K) mice with selective reduction of the Navbeta2 intracellular domain (NICD), we combined behavioral phenotyping, electrophysiology, neurotransmitter profiling, and molecular analyses, with chronic unpredictable mild stress (CUMS) as an environmental challenge. SCN2B-OV mice exhibited accelerated depression-like phenotypes, including anhedonia (sucrose preference 52.42% reduction, P<0.05), behavioral despair (forced swim test immobility 67.42% increase, P<0.05), and cognitive deficits (Y-maze alternation 50.90% reduction, P<0.05), independent of natural aging. Electrophysiologically, SCN2B overexpression induced a striking prefrontal-hippocampal imbalance: prefrontal cortex (PFC) became hyperexcitable (glutamate release increased by 64.7%; sEPSC amplitude increased 2.05-fold, P<0.05), whereas the hippocampus showed synaptic suppression (GABA release reduced by 32.65%; sIPSC frequency reduced by 66.67%, P<0.05). Mechanistically, SCN2B overexpression suppressed BDNF/TrkB/PI3K/AKT signaling axis (BDNF reduced by 53.17%; p-TrkB reduced by 63.62%, P<0.05), accompanied by decreased synaptic proteins (PSD95 43.45%, synapsin I 40.31%, P<0.05). Remarkably, NICD-reduced Navbeta2(V173A,V175K) mice were fully resistant to CUMS-induced behavioral and neurotransmitter deficits, establishing NICD as a critical mediator of stress vulnerability. Collectively, these findings unravel a causal pathway in which SCN2B overexpression drives NICD-dependent suppression of BDNF signaling, leading to region-specific excitatory/inhibitory imbalance, synaptic disintegration, and depression-like phenotypes. Targeting Navbeta2 proteolysis to block NICD generation may thus represent a novel therapeutic strategy for mood disorders. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Lu, Min-Nan AU - Lu MN AD - Institute of Neuroscience, Kunming Medical University, Kunming, 650500, Yunnan, China; Science and Technology Achievement Incubation Center, Kunming Medical University, Kunming, Yunnan, 650500, China. Electronic address: luminnan@kmmu.edu.cn. FAU - Yan, Guo-Ji AU - Yan GJ AD - Institute of Neuroscience, Kunming Medical University, Kunming, 650500, Yunnan, China. Electronic address: 20200027@kmmu.edu.cn. FAU - Wang, Dan AU - Wang D AD - Institute of Neuroscience, Kunming Medical University, Kunming, 650500, Yunnan, China. Electronic address: 20210030@kmmu.edu.com. FAU - Huang, Hong-Chen AU - Huang HC AD - Institute of Neuroscience, Kunming Medical University, Kunming, 650500, Yunnan, China. Electronic address: 18986954087@163.com. FAU - Zhou, Ying AU - Zhou Y AD - Electron Microscopy Laboratory, Scientific Research Experiment Center, Kunming Medical University, Kunming, Yunnan, 650500, China. Electronic address: zynl1990@163.com. FAU - Li, Shan-Shan AU - Li SS AD - Experimental Teaching Center, Basic Medical College, Kunming Medical University, Kunming, Yunnan 650500, China. Electronic address: 94186610@qq.com. FAU - Ye, Chen-Jun AU - Ye CJ AD - Institute of Neuroscience, Kunming Medical University, Kunming, 650500, Yunnan, China. Electronic address: yechenjun@kmmu.edu.cn. FAU - Dong, Xiao-Han AU - Dong XH AD - Institute of Neuroscience, Kunming Medical University, Kunming, 650500, Yunnan, China. Electronic address: xiaohan13460@126.com. FAU - Yang, Chen-Di AU - Yang CD AD - Institute of Neuroscience, Kunming Medical University, Kunming, 650500, Yunnan, China. Electronic address: 976435455@qq.com. FAU - Liang, Xi-Wen AU - Liang XW AD - Institute of Neuroscience, Kunming Medical University, Kunming, 650500, Yunnan, China. Electronic address: liangxw188@126.com. FAU - Niu, Yong-Min AU - Niu YM AD - Institute of Neuroscience, Kunming Medical University, Kunming, 650500, Yunnan, China. Electronic address: 421089982@qq.com. FAU - Hu, Tao AU - Hu T AD - Department of Laboratory Medicine, The Third People's Hospital of Yunnan Province, Kunming, China. Electronic address: 18134318@qq.com. FAU - Yang, Xin-Wang AU - Yang XW AD - Department of Anatomy and Histology and Embryology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, Yunnan, 650500, China. Electronic address: yangxinwanghp@163.com. FAU - Xiyang, Yan-Bin AU - Xiyang YB AD - Institute of Neuroscience, Kunming Medical University, Kunming, 650500, Yunnan, China. Electronic address: xiyangyanbin@kmmu.edu.cn. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Neuropharmacology JT - Neuropharmacology JID - 0236217 SB - IM OTO - NOTNLM OT - BDNF OT - Depression OT - Excitatory-inhibitory balance OT - NICD OT - Neuroplasticity OT - SCN2B COIS- Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:37 PHST- 2026/05/12 00:00 [received] PHST- 2026/10/22 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:37 [entrez] AID - S0028-3908(26)00417-X [pii] AID - 10.1016/j.neuropharm.2026.111242 [doi] PST - aheadofprint SO - Neuropharmacology. 2026 Oct 28:111242. doi: 10.1016/j.neuropharm.2026.111242. PMID- 42951242 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-7064 (Electronic) IS - 0028-3908 (Linking) VI - 303 DP - 2026 Oct 28 TI - Ligand-specific effects of 5-HT2A receptor antagonists on fear extinction in C57BL/6J mice: Comparative insights from MDL 11,939 and MDL 100,907. PG - 111243 LID - S0028-3908(26)00418-1 [pii] LID - 10.1016/j.neuropharm.2026.111243 [doi] AB - Serotonin (5-HT) 2A receptors (5-HT(2)AR) modulate corticolimbic circuits underlying fear extinction, but the behavioral consequences of 5-HT(2)AR antagonism during extinction remain poorly defined. Here, we examined the systemic effects of the 5-HT(2)A/(2)C antagonist MDL 11,939 (Glemanserin) and the selective 5-HT(2)A antagonist MDL 100,907 (Volinanserin) on fear extinction in adult female and male C57BL/6J mice. Prior to drug administration, mice acquired comparable conditioned freezing responses during delay fear conditioning. Twenty-four hours later, acute administration of MDL 11,939 (1.0 mg/kg) or MDL 100,907 (0.01 mg/kg) increased freezing to the first conditioned stimulus (CS) presentation on Extinction Day 1 in males, indicating enhanced expression of conditioned fear. Repeated administration of MDL 11,939 significantly impaired extinction in male mice, evidenced by increased freezing across extinction trials and an increased number of trials required to reach extinction criterion. Neither acute nor repeated MDL 11,939 altered extinction in female mice. In males, MDL 100,907 produced a transient increase in freezing during extinction but did not delay extinction acquisition and had no effect in females. Because MDL 11,939 has reported affinity for 5-HT(2)C receptors, we tested potential contributions of 5-HT(2)C receptor antagonism in a separate cohort of male mice given repeated administration of the selective 5-HT(2)C antagonist SB 242084 (0.25 or 0.5 mg/kg). Neither dose affected conditioned fear expression, extinction learning, or trials required to reach extinction criterion. Together, these findings demonstrate ligand-, sex-, and dose-dependent effects of 5-HT(2)AR antagonism on fear extinction and suggest that distinct intracellular receptor signaling mechanisms may differentially regulate extinction-related behavior. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Tyulmenkova, Anastasia AU - Tyulmenkova A AD - Department of Biological Sciences, Charles E. Schmidt College of Science, Florida Atlantic University, Jupiter, FL, 33458, USA. FAU - Stackman, Robert W Jr AU - Stackman RW Jr AD - Department of Biological Sciences, Charles E. Schmidt College of Science, Florida Atlantic University, Jupiter, FL, 33458, USA; Department of Psychology, Charles E. Schmidt College of Science, Florida Atlantic University, Jupiter, FL, 33458, USA. Electronic address: rstackma@fau.edu. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Neuropharmacology JT - Neuropharmacology JID - 0236217 SB - IM OTO - NOTNLM OT - 5-HT(2)A receptor OT - Antagonism OT - Fear extinction OT - MDL OT - Serotonin COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:37 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 20:37 [entrez] AID - S0028-3908(26)00418-1 [pii] AID - 10.1016/j.neuropharm.2026.111243 [doi] PST - aheadofprint SO - Neuropharmacology. 2026 Oct 28;303:111243. doi: 10.1016/j.neuropharm.2026.111243. PMID- 42951230 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-1597 (Electronic) IS - 1572-1000 (Linking) DP - 2026 Oct 28 TI - Optical Assessment of Corneal Endothelial Morphology in Treatment-Naive Dysthyroid Patients: A Specular Microscopy Analysis. PG - 105699 LID - S1572-1000(26)00364-9 [pii] LID - 10.1016/j.pdpdt.2026.105699 [doi] AB - PURPOSE: To evaluate corneal endothelial morphology in treatment-naive dysthyroid patients using age-adjusted analysis and identify diagnostic thresholds. METHODS: This prospective case-control study enrolled 46 treatment-naive dysthyroid patients (27 hyperthyroid, 19 hypothyroid) and 48 controls. Optical imaging of endothelial parameters (ECD, CV, hexagonality, average cell area, CCT) was performed using non-contact specular microscopy, a non-invasive optical assessment technique. Age-adjusted ANCOVA with formal assumption testing, ROC analysis with bootstrap confidence intervals, and a left eye sensitivity analysis were performed. RESULTS: Hexagonality was significantly lower in both hyperthyroid (56.56+/-7.19% vs 62.54+/-4.30%; d=-1.09; p=0.003) and hypothyroid groups (56.42+/-8.01% vs controls; d=-1.10; p=0.027). Average cell area was elevated in both groups (d>1.1; p/=275mum(2)) with a combined model AUC of 0.828 (optimism-corrected 0.812). Left eye sensitivity analysis confirmed the concordance of HEX and AVG findings across both eyes, while CV showed discordant laterality. ECD and CCT were preserved. CONCLUSION: Treatment-naive dysthyroid patients showed age-independent endothelial morphological alterations despite preserved cell density. Optical assessment via specular microscopy with ROC-derived cutoffs offers preliminary clinical utility for preoperative risk stratification in dysthyroid patients, pending external validation. Findings in the hypothyroid subgroup, which did not reach the planned sample size, should be considered hypothesis-generating. CI - Copyright (c) 2026 The Author(s). Published by Elsevier B.V. All rights reserved. FAU - Bayram, Ugur AU - Bayram U AD - Department of Ophthalmology, Giresun University Faculty of Medicine, Giresun, Turkey. FAU - Ozen, Serkan AU - Ozen S AD - Department of Ophthalmology, Giresun University Faculty of Medicine, Giresun, Turkey. Electronic address: serkan_zen@hotmail.com. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Photodiagnosis Photodyn Ther JT - Photodiagnosis and photodynamic therapy JID - 101226123 SB - IM OTO - NOTNLM OT - corneal endothelium OT - hexagonality OT - optical imaging OT - specular microscopy OT - thyroid dysfunction OT - treatment-naive COIS- Declaration of competing interest The authors declare no conflicts of interest. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S1572-1000(26)00364-9 [pii] AID - 10.1016/j.pdpdt.2026.105699 [doi] PST - aheadofprint SO - Photodiagnosis Photodyn Ther. 2026 Oct 28:105699. doi: 10.1016/j.pdpdt.2026.105699. PMID- 42951229 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-3913 (Electronic) IS - 0898-6568 (Linking) DP - 2026 Oct 28 TI - NMI suppresses BEFV replication and is degraded by BEFV-induced NIX-dependent selective autophagy. PG - 112976 LID - S0898-6568(26)00636-4 [pii] LID - 10.1016/j.cellsig.2026.112976 [doi] AB - N-Myc interactor (NMI) functions in various viral infections and serves as a regulatory component of innate immune signaling, its function during bovine ephemeral fever virus (BEFV) infection remains to be elucidated. Herein, we establish that NMI attenuates BEFV replication in BHK-21 and MDBK cells, as evidenced by diminished viral nucleoprotein gene expression and viral loads in NMI-overexpressing cells, whereas NMI depletion significantly enhances viral replication. Further analyses reveal that NMI augments type I interferon (IFN-I) pathway activation via the transcriptional induction of Ifnb and downstream effectors, namely Isg15, Ifitm3, Mx1, Isg54 and Isg56. At the molecular level, NMI engages with IRF3, facilitating its phosphorylation and subsequent nuclear import, consequently potentiating IFN-I-mediated immune activation. Notably, BEFV infection counteracts this antiviral defense by inducing autophagy, resulting in time-dependent autophagic degradation of NMI. Further analysis demonstrates that BEFV-mediated NMI degradation occurs selectively through the autophagy receptor NIP3-like protein X (NIX), as pharmacological inhibition of autophagy or NIX knockout abrogates NMI degradation, while NIX overexpression accelerates NMI turnover. Collectively, our results delineate a novel molecular mechanism whereby NMI restricts BEFV replication through the IRF3-IFN axis, while BEFV evades this restriction via NIX-mediated selective autophagy. These observations furnish critical insights into NMI function and the dynamic crosstalk between host antiviral defenses and viral immune evasion mechanisms, which may lay the groundwork for the rational design of antiviral therapeutics. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Zhu, Hongchao AU - Zhu H AD - Ruminant Diseases Research Center, College of Life Sciences, Shandong Normal University, Jinan, Shandong 250358, China. FAU - Sun, Xiaonan AU - Sun X AD - Ruminant Diseases Research Center, College of Life Sciences, Shandong Normal University, Jinan, Shandong 250358, China. FAU - Zhang, Yangyang AU - Zhang Y AD - Ruminant Diseases Research Center, College of Life Sciences, Shandong Normal University, Jinan, Shandong 250358, China. FAU - Sun, Xiaorui AU - Sun X AD - Ruminant Diseases Research Center, College of Life Sciences, Shandong Normal University, Jinan, Shandong 250358, China. FAU - Wang, Hongmei AU - Wang H AD - Ruminant Diseases Research Center, College of Life Sciences, Shandong Normal University, Jinan, Shandong 250358, China. Electronic address: hongmeiwang@sdnu.edu.cn. FAU - He, Hongbin AU - He H AD - Ruminant Diseases Research Center, College of Life Sciences, Shandong Normal University, Jinan, Shandong 250358, China. Electronic address: hongbinhe@sdnu.edu.cn. FAU - Hou, Peili AU - Hou P AD - Ruminant Diseases Research Center, College of Life Sciences, Shandong Normal University, Jinan, Shandong 250358, China. Electronic address: peilihou@sdnu.edu.cn. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Cell Signal JT - Cellular signalling JID - 8904683 SB - IM OTO - NOTNLM OT - Autophagy OT - BEFV OT - IFN response OT - NMI COIS- Declaration of competing interest The authors declare that they have no competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/07/29 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S0898-6568(26)00636-4 [pii] AID - 10.1016/j.cellsig.2026.112976 [doi] PST - aheadofprint SO - Cell Signal. 2026 Oct 28:112976. doi: 10.1016/j.cellsig.2026.112976. PMID- 42951228 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-3913 (Electronic) IS - 0898-6568 (Linking) DP - 2026 Oct 28 TI - P2X7 receptor-mediated inflammatory and ionic remodeling promotes atrial fibrillation in diabetes. PG - 112973 LID - S0898-6568(26)00633-9 [pii] LID - 10.1016/j.cellsig.2026.112973 [doi] AB - AIMS: Diabetes mellitus (DM) is a major risk factor for atrial fibrillation (AF), yet the mechanisms by which diabetic metabolic stress promotes atrial arrhythmogenesis remain incompletely understood. Recent evidence suggests that purinergic signaling-driven sterile inflammation plays an important role in cardiac remodeling. This study aimed to investigate the role of the ATP-gated purinergic P2X7 receptor (P2X7R) in DM-associated AF and to explore its underlying mechanisms. METHODS AND RESULTS: Rats were randomly assigned to four groups: control (Con), diabetes mellitus (DM), P2X7R overexpression in controls (Con+P2X7), and P2X7R knockdown in diabetic rats (DM + P2X7 KD), with gene manipulation achieved using AAV9-cTnT vectors. AF susceptibility, atrial structural and electrical remodeling were systematically evaluated using electrophysiological studies, optical mapping, patch-clamp recordings, and molecular analyses. DM markedly increased AF inducibility and duration, accompanied by enhanced sympathetic nerve activity and activation of P2X7R-related inflammatory signaling. Compared with controls, DM atria exhibited pronounced interstitial fibrosis, connexin remodeling, delayed atrial activation, slowed conduction velocity, and increased conduction heterogeneity. Atrial P2X7R overexpression in control rats recapitulated key arrhythmogenic features, whereas P2X7R KD in diabetic rats significantly attenuated atrial remodeling and reduced AF susceptibility, without altering the diabetes-induced increase in sympathetic activity. At the cellular level, P2X7R selectively modulated I(Na), I(Ca,L), and I(Kur) while relatively preserving I(to), and was associated with mitochondrial dysfunction and disturbed Ca(2+) homeostasis, characterized by increased mitochondrial reactive oxygen species, loss of mitochondrial membrane potential, increased cytosolic Ca(2+) signals, and reduced mitochondrial Ca(2+) uptake. CONCLUSION: P2X7R-mediated inflammatory and mitochondrial signaling contribute to diabetic atrial remodeling and AF susceptibility. Targeting P2X7R may represent a potential therapeutic strategy for preventing AF in diabetes. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Fu, Yuntao AU - Fu Y AD - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR China; Cardiovascular Research Institute, Wuhan University, Wuhan 430060, PR China; Hubei Key Laboratory of Cardiology, Wuhan 430060, PR China. FAU - Ke, Yuanjia AU - Ke Y AD - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR China; Cardiovascular Research Institute, Wuhan University, Wuhan 430060, PR China; Hubei Key Laboratory of Cardiology, Wuhan 430060, PR China. FAU - Cao, Zhen AU - Cao Z AD - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR China; Cardiovascular Research Institute, Wuhan University, Wuhan 430060, PR China; Hubei Key Laboratory of Cardiology, Wuhan 430060, PR China. FAU - Chen, Yuting AU - Chen Y AD - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR China; Cardiovascular Research Institute, Wuhan University, Wuhan 430060, PR China; Hubei Key Laboratory of Cardiology, Wuhan 430060, PR China. FAU - Long, Xiaojian AU - Long X AD - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR China; Cardiovascular Research Institute, Wuhan University, Wuhan 430060, PR China; Hubei Key Laboratory of Cardiology, Wuhan 430060, PR China. FAU - Li, Yajia AU - Li Y AD - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR China; Cardiovascular Research Institute, Wuhan University, Wuhan 430060, PR China; Hubei Key Laboratory of Cardiology, Wuhan 430060, PR China. FAU - Guo, Kexin AU - Guo K AD - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR China; Cardiovascular Research Institute, Wuhan University, Wuhan 430060, PR China; Hubei Key Laboratory of Cardiology, Wuhan 430060, PR China. FAU - Luo, Yixuan AU - Luo Y AD - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR China; Cardiovascular Research Institute, Wuhan University, Wuhan 430060, PR China; Hubei Key Laboratory of Cardiology, Wuhan 430060, PR China. FAU - Hu, Kaigeng AU - Hu K AD - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR China; Cardiovascular Research Institute, Wuhan University, Wuhan 430060, PR China; Hubei Key Laboratory of Cardiology, Wuhan 430060, PR China. FAU - Zhao, Qingyan AU - Zhao Q AD - Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan 430060, PR China; Cardiovascular Research Institute, Wuhan University, Wuhan 430060, PR China; Hubei Key Laboratory of Cardiology, Wuhan 430060, PR China. Electronic address: ruyan71@163.com. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Cell Signal JT - Cellular signalling JID - 8904683 SB - IM OTO - NOTNLM OT - Atrial fibrillation OT - Calcium homeostasis OT - Diabetes mellitus OT - Inflammation OT - Mitochondrial damage OT - P2X7 receptor COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this manuscript. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/06/25 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S0898-6568(26)00633-9 [pii] AID - 10.1016/j.cellsig.2026.112973 [doi] PST - aheadofprint SO - Cell Signal. 2026 Oct 28:112973. doi: 10.1016/j.cellsig.2026.112973. PMID- 42951224 OWN - NLM STAT- Publisher LR - 20261028 IS - 1095-9572 (Electronic) IS - 1053-8119 (Linking) DP - 2026 Oct 28 TI - Impact of hearing loss on visual selective attention: Evidence from N2pc and resting-state networks. PG - 122321 LID - S1053-8119(26)00636-1 [pii] LID - 10.1016/j.neuroimage.2026.122321 [doi] AB - Efficient visual attention is fundamental to environmental adaptation and social interaction. While previous studies have observed alterations in visual processing among deaf individuals, findings remain controversial regarding whether these changes stem from hearing loss (sensory compensation vs. cognitive deficit) or sign language experience. To address this issue, the present study provides task-related and resting-state electroencephalography (EEG) evidence to disentangle the specific drivers of visual selective attention plasticity. A visual search task was used to examine selective attention in 95 participants (34 prelingual deaf, 27 hearing signers, and 34 hearing nonsigners). Compared to both hearing groups, deaf individuals exhibited significantly lower hit rates and diminished N2-posterior contralateral (N2pc) amplitudes. Hearing signers and nonsigners showed comparable behavioral performance, N2pc amplitudes, and resting-state network topology, whereas the deaf group was further characterized by a degraded resting-state alpha network topology, evidenced by reduced clustering coefficients, global efficiency, and weakened frontal-occipital connectivity. N2pc amplitude correlated significantly with resting-state network properties and behavioral accuracy. These results suggest that deaf individuals may suffer from an imbalance in the regulation of visual selective attention, most clearly indexed by the evoked N2pc and by intrinsic alpha-network organization, and likely linked to increased resource competition following the loss of auditory monitoring. Our findings provide neurophysiological evidence that these attentional deficits are associated with hearing loss rather than with sign-language experience, offering a new perspective for understanding the boundaries of cross-modal plasticity in the human brain. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Zeng, Tongao AU - Zeng T AD - School of Education Science, Hunan Normal University, Changsha, China; Department of Special Education, Faculty of Education, East China Normal University, Shanghai, China; Tibet Autonomous Region Key Laboratory for High Altitude Brain Science and Environmental Acclimatization, Tibet University, Lhasa, China. FAU - Bu, Xiaoou AU - Bu X AD - School of Special Education, Nanjing Normal University of Special Education, Nanjing, China. FAU - Zhu, Han AU - Zhu H AD - Department of Special Education, Faculty of Education, East China Normal University, Shanghai, China. FAU - Liu, Ziyao AU - Liu Z AD - Department of Special Education, Faculty of Education, East China Normal University, Shanghai, China. FAU - Xiao, Hongli AU - Xiao H AD - Department of Special Education, Faculty of Education, East China Normal University, Shanghai, China. FAU - Li, Fuxiang AU - Li F AD - Department of Special Education, Faculty of Education, East China Normal University, Shanghai, China. FAU - Zhang, Wenjun AU - Zhang W AD - Department of Special Education, Faculty of Education, East China Normal University, Shanghai, China. FAU - Yang, Fuyi AU - Yang F AD - Department of Special Education, Faculty of Education, East China Normal University, Shanghai, China. FAU - Chen, Jie AU - Chen J AD - School of Education Science, Hunan Normal University, Changsha, China. Electronic address: xlxchen@163.com. FAU - Ma, Hailin AU - Ma H AD - Tibet Autonomous Region Key Laboratory for High Altitude Brain Science and Environmental Acclimatization, Tibet University, Lhasa, China; Plateau Brain Science Research Center, Tibet University, Lhasa, China; Qinghai Cardio-Cerebrovascular Specialty Hospital, Xining, China. Electronic address: David_ma79@163.com. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Neuroimage JT - NeuroImage JID - 9215515 SB - IM OTO - NOTNLM OT - Deafness OT - N2pc OT - Resting-state EEG network OT - Sign language experience OT - Visual selective attention COIS- Declaration of competing interest The authors report no conflict of interest. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/03/24 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S1053-8119(26)00636-1 [pii] AID - 10.1016/j.neuroimage.2026.122321 [doi] PST - aheadofprint SO - Neuroimage. 2026 Oct 28:122321. doi: 10.1016/j.neuroimage.2026.122321. PMID- 42951223 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-3476 (Electronic) IS - 0378-5173 (Linking) DP - 2026 Oct 28 TI - Enhanced inhalation delivery of ROS-responsive methylprednisolone dimer nanoparticles via hydrogen-oxygen carrier gas for efficient treatment of acute lung injury with CFD-simulated pulmonary deposition. PG - 127568 LID - S0378-5173(26)01017-3 [pii] LID - 10.1016/j.ijpharm.2026.127568 [doi] AB - Distal lung delivery remains a key bottleneck for inhaled therapy in severe pulmonary disease. We developed a hydrogen-oxygen mixture (H(2)/O(2) mixture, 2:1 v/v) gas-assisted nebulization platform to deliver ROS-responsive, self-assembled methylprednisolone dimer nanoparticles (MP2-TK NPs). MP2-TK NPs were stable during nebulization and selectively released drug under ROS-rich conditions. Compared with air, H(2)/O(2) mixture improved NGI-assessed aerosol deposition and artificial mucus penetration. CFD simulations further supported enhanced distal deposition, particularly for clinically respirable droplets (1-5 mum). In an LPS-induced acute lung injury mouse model, H(2)/O(2)-assisted nebulization increased pulmonary distribution and small-airway localization of MP2-TK NPs, leading to reduced bronchoalveolar lavage cytokines (IL-6, TNF-alpha, IL-1beta), total cell counts, and protein levels. H(2)/O(2) mixture inhalation alone also showed anti-inflammatory activity, consistent with hydrogen's antioxidant effects. This strategy couples carrier-gas engineering with ROS-responsive nanomedicine to enhance deep-lung delivery and therapeutic efficacy in acute lung injury. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Tian, Xidong AU - Tian X AD - State Key Laboratory of Mechanism and Quality of Chinese Medicine, University of Macau, Macau 999078, China; Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China. FAU - Li, Bogang AU - Li B AD - State Key Laboratory of Mechanism and Quality of Chinese Medicine, University of Macau, Macau 999078, China; Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China. FAU - Chen, Jiamao AU - Chen J AD - State Key Laboratory of Mechanism and Quality of Chinese Medicine, University of Macau, Macau 999078, China; Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China. FAU - Zha, Haidong AU - Zha H AD - State Key Laboratory of Mechanism and Quality of Chinese Medicine, University of Macau, Macau 999078, China; Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China. FAU - Liu, Chang AU - Liu C AD - School of Pharmacy, Shenyang Key Laboratory of Intelligent Mucosal Drug Delivery Systems, Shenyang Pharmaceutical University, Shenyang 110016, China. FAU - Guan, Jianhuan AU - Guan J AD - State Key Laboratory of Mechanism and Quality of Chinese Medicine, University of Macau, Macau 999078, China; Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China. FAU - Liu, Tzu-Ming AU - Liu TM AD - Institute of Translational Medicine, Faculty of Medicine Frontiers Science Center for Precision Oncology, University of Macau, Macau 999078, China. FAU - Ouyang, Defang AU - Ouyang D AD - State Key Laboratory of Mechanism and Quality of Chinese Medicine, University of Macau, Macau 999078, China; Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China; Department of Biological Sciences, Faculty of Science, University of Macau, Macau 999078, China. Electronic address: defangouyang@um.edu.mo. FAU - Zheng, Ying AU - Zheng Y AD - State Key Laboratory of Mechanism and Quality of Chinese Medicine, University of Macau, Macau 999078, China; Institute of Chinese Medical Sciences, University of Macau, Macau 999078, China; Department of Pharmacy and Pharmaceutical Sciences, Faculty of Meidicine, University of Macau, Macau 999078, China. Electronic address: yzheng@um.edu.mo. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Int J Pharm JT - International journal of pharmaceutics JID - 7804127 SB - IM OTO - NOTNLM OT - Acute lung injury OT - CFD-simulated pulmonary deposition OT - Carrier-gas engineering OT - Hydrogen-oxygen mixed gas OT - Inhalation drug delivery OT - ROS-responsivenanoprodrug COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S0378-5173(26)01017-3 [pii] AID - 10.1016/j.ijpharm.2026.127568 [doi] PST - aheadofprint SO - Int J Pharm. 2026 Oct 28:127568. doi: 10.1016/j.ijpharm.2026.127568. PMID- 42951220 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-3476 (Electronic) IS - 0378-5173 (Linking) DP - 2026 Oct 28 TI - The photostability of ketamine products: part ii impact of light exposure and storage on multi-use ketamine parenteral products. PG - 127544 LID - S0378-5173(26)00993-2 [pii] LID - 10.1016/j.ijpharm.2026.127544 [doi] AB - Ketamine drug products for parenteral use are commonly packaged in multi-use clear glass vials. However, light exposure may cause formation of N-nitroso-ketamine (NNKet), a potential carcinogen and nitrosamine drug substance-related impurity (NDSRI) of regulatory concern. To better understand ketamine photostability and nitrosamine formation potential, a photostability investigation was performed using a two-stage approach evaluating ambient and intense light exposure followed by post-exposure storage. This design reflects clinical reality where multi-use injectable ketamine experiences multiple intermittent light exposures during use. A selective and sensitive UHPLC-MS/MS analytical procedure was developed and validated for NNKet quantification. Sample preparation required solid phase extraction (SPE) to separate NNKet from ketamine before LC-MS/MS measurement, essential for overcoming autosampler contamination and ionization suppression from million-fold excess ketamine. Unexpectedly, NNKet concentration initially decreased during light exposure but rapidly increased during post-exposure storage. NNKet increased from baseline levels of approximately 5 ng/mL and exceeded the lowest acceptable intake limit of 14 ng/mL for 10 mg/mL ketamine drug product, reaching 100 ng/mL in some cases within 8 weeks of post-exposure storage. Control samples (light-protected vials) showed minimal NNKet change (maximum increase of 4.7 ng/mL from baseline). Kinetic modeling and experiments were attempted to explain the underlying mechanism. These findings demonstrate a post-market safety concern for ketamine drug products stored in clear glass vials, suggesting that container-closure systems minimizing light exposure, such as amber glass vials, may help mitigate nitrosamine formation risks in ketamine drug products. CI - Published by Elsevier B.V. FAU - Wang, Hairong AU - Wang H AD - Office of Pharmaceutical Quality Research, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Life Science Building-64, Silver Spring, MD 20993, USA. FAU - Zou, Guozhang AU - Zou G AD - Office of Pharmaceutical Quality Research, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Life Science Building-64, Silver Spring, MD 20993, USA. FAU - Liang, Yuanling AU - Liang Y AD - Office of Pharmaceutical Quality Assessment, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD 20993, USA. FAU - Gu, Jianghong AU - Gu J AD - Office of Pharmaceutical Quality Research, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Life Science Building-64, Silver Spring, MD 20993, USA. FAU - Lupitskyy, Robert AU - Lupitskyy R AD - Office of Pharmaceutical Quality Research, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Life Science Building-64, Silver Spring, MD 20993, USA. FAU - Dong, Xiangyi AU - Dong X AD - Office of Pharmaceutical Quality Research, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Life Science Building-64, Silver Spring, MD 20993, USA. FAU - Essandoh, Martha AU - Essandoh M AD - Office of Pharmaceutical Quality Research, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Life Science Building-64, Silver Spring, MD 20993, USA. FAU - Shakleya, Diaa AU - Shakleya D AD - Office of Pharmaceutical Quality Research, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Life Science Building-64, Silver Spring, MD 20993, USA. FAU - Xu, Xiaoming AU - Xu X AD - Office of Pharmaceutical Quality Research, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Life Science Building-64, Silver Spring, MD 20993, USA. FAU - Keire, David AU - Keire D AD - Office of Pharmaceutical Quality Research, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Life Science Building-64, Silver Spring, MD 20993, USA. FAU - Faustino, Patrick AU - Faustino P AD - Office of Pharmaceutical Quality Research, Office of Pharmaceutical Quality, Center for Drug Evaluation and Research, U.S. Food and Drug Administration, Life Science Building-64, Silver Spring, MD 20993, USA. Electronic address: Patrick.Faustino@fda.hhs.gov. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Int J Pharm JT - International journal of pharmaceutics JID - 7804127 SB - IM OTO - NOTNLM OT - Ketamine OT - Light exposure OT - N-nitroso-ketamine OT - NDSRIs OT - Nitrosamine OT - Photostability COIS- Declaration of competing interest The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/04/20 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S0378-5173(26)00993-2 [pii] AID - 10.1016/j.ijpharm.2026.127544 [doi] PST - aheadofprint SO - Int J Pharm. 2026 Oct 28:127544. doi: 10.1016/j.ijpharm.2026.127544. PMID- 42951209 OWN - NLM STAT- Publisher LR - 20261028 IS - 2341-1929 (Electronic) IS - 2341-1929 (Linking) DP - 2026 Oct 28 TI - Evidence for formulating recommendations on the use of desflurane vs sevoflurane and propofol in general anaesthesia: an umbrella review. PG - 502163 LID - S2341-1929(26)00199-X [pii] LID - 10.1016/j.redare.2026.502163 [doi] AB - OBJECTIVE: To evaluate the efficacy, effectiveness, and safety of desflurane compared with sevoflurane and propofol for the maintenance of general anaesthesia across different patient subgroups. METHODS: Umbrella review including evidence synthesis studies published between 2014 and 2024. Methodological quality was assessed using AMSTAR 2, and certainty of evidence using the GRADE approach. RESULTS: Thirty-one systematic reviews were included. Desflurane was consistently associated with faster recovery than sevoflurane across the analysed subgroups, although the certainty of evidence was low or very low. Compared with propofol, favourable findings for desflurane in selected early recovery outcomes were less consistent. No clinically relevant differences were identified for morbidity outcomes, except for a possible advantage of propofol in postoperative nausea and vomiting and emergence agitation. CONCLUSIONS: Desflurane may be conditionally considered when rapid recovery is prioritized, with individualized decision-making required. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Reinoso Barbero, F AU - Reinoso Barbero F AD - Servicio de Anestesiologia Pediatrica, Cuidados Criticos y Dolor, Hospital Universitario La Paz, Madrid. FAU - Benot Lopez, S AU - Benot Lopez S AD - Comision de Evaluacion de Tecnologias Sanitarias del Servicio Andaluz de Salud (SAS), Hospital Universitario Virgen del Rocio, Sevilla. FAU - Candia Arana, C A AU - Candia Arana CA AD - Servicio de Anestesiologia y Reanimacion y Tratamiento del Dolor, Complejo Hospitalario Universitario de Cartagena, Murcia. FAU - Abad Gurumeta, A AU - Abad Gurumeta A AD - Servicio de Anestesiologia y Reanimacion y Tratamiento del Dolor, Hospital Universitario Infanta Leonor, Madrid. FAU - Marin, L AU - Marin L AD - Anestesiologia, Berna, Switzerland. FAU - Hernandez Bisshopp, R AU - Hernandez Bisshopp R AD - Servicio de Anestesiologia, Reanimacion y Tratamiento del Dolor, Hospital Universitario Nuestra Senora de Candelaria, Santa Cruz de Tenerife. FAU - Salazar Bonilla, C J AU - Salazar Bonilla CJ AD - Servicio de Anestesiologia, Reanimacion y Tratamiento del Dolor, Hospital Universitario Nuestra Senora de Candelaria, Santa Cruz de Tenerife. FAU - Aliaga Medina, J AU - Aliaga Medina J AD - Servicio de Anestesiologia, Reanimacion y Tratamiento del Dolor, Hospital Clinic, Barcelona. FAU - Sanabria Carretero, P AU - Sanabria Carretero P AD - Servicio de Anestesiologia Pediatrica, Cuidados Criticos y Dolor, Hospital Universitario La Paz, Madrid. FAU - Varela Duran, M AU - Varela Duran M AD - Servicio de Anestesiologia, Reanimacion y Terapia del Dolor, Hospital Montecelo, Area Sanitaria de Pontevedra e O Salnes, Pontevedra. FAU - Reinoso Camino, M AU - Reinoso Camino M AD - Servicio de Medicina Preventiva y Salud Publica, Hospital Virgen de las Nieves, Granada. FAU - de la Matta, M AU - de la Matta M AD - Servicio de Anestesiologia Reanimacion y Tratamiento del Dolor, Hospital Universitario Virgen del Rocio, Sevilla. Electronic address: mdlmatta@hotmail.com. CN - FRB, CCA, AAG, RHB, CJSB, JAM, PSC, MVD, MM, LM son miembros del Grupo de Trabajo en Sostenibilidad de la SEDAR. MVD, MM y LM ejercen las funciones de coordinadores de este LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - Spain TA - Rev Esp Anestesiol Reanim (Engl Ed) JT - Revista espanola de anestesiologia y reanimacion JID - 101778594 SB - IM OTO - NOTNLM OT - Anestesia general OT - Desflurano OT - Propofol OT - Recuperacion postoperatoria OT - Revision sistematica OT - Sevoflurano OT - desflurane OT - general anaesthesia OT - postoperative recovery OT - propofol OT - sevoflurane OT - systematic review EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/02/02 00:00 [received] PHST- 2026/04/13 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S2341-1929(26)00199-X [pii] AID - 10.1016/j.redare.2026.502163 [doi] PST - aheadofprint SO - Rev Esp Anestesiol Reanim (Engl Ed). 2026 Oct 28:502163. doi: 10.1016/j.redare.2026.502163. PMID- 42951206 OWN - NLM STAT- Publisher LR - 20261028 IS - 2212-8778 (Electronic) IS - 2212-8778 (Linking) DP - 2026 Oct 28 TI - Regular exercise augments gut-brain communication and visceral feedback control of appetite. PG - 102463 LID - S2212-8778(26)00147-X [pii] LID - 10.1016/j.molmet.2026.102463 [doi] AB - OBJECTIVES: Regular exercise is widely accepted to support long-term body weight management; however, the neurobiological adaptations that contribute to the beneficial effects of habitual physical activity beyond increases in energy expenditure per se remain unclear. Here, we investigated whether regular exercise alters gut-brain communication and visceral feedback control of feeding. METHODS: Mice were subjected to regular voluntary wheel running. Energy expenditure and feeding were assessed under basal conditions and following an overnight fast. Nutrients were delivered directly into the stomach by intragastric infusion. Brain-wide responses to intragastric nutrient delivery were assessed using positron emission tomography (PET). Single-cell sequencing and histological analyses were used to determine nutrient-responsive neuronal populations in the caudal brainstem. Genetic associations with body weight were evaluated using GWAS data. RESULTS: Intragastric nutrient delivery suppressed feeding more strongly in exercising mice. Brain-wide PET revealed enhanced responses to intragastric nutrients in various brain regions. Single-cell sequencing identified several nutrient-responsive caudal brainstem populations, including a previously unrecognized population marked by expression of fibrinogen C domain containing 1 (FIBCD1). Regular exercise selectively enhanced the nutrient-evoked activation of FIBCD1 neurons. Histological analyses demonstrate that FIBCD1 neurons are also present in the human brainstem, and GWAS data suggest a potential relevance of this neuron population to body-weight regulation. CONCLUSIONS: Regular exercise enhances behavioral and brain responses to intragastric nutrient delivery and sensitizes FIBCD1 neurons in the brainstem of mice. These findings reveal an exercise-associated adaptation of gut-brain communication that may contribute to improved matching of caloric intake to energy expenditure. CI - Copyright (c) 2026 The Author(s). Published by Elsevier GmbH.. All rights reserved. FAU - Borgmann, Diba AU - Borgmann D AD - Novo Nordisk Foundation Center for Basic Metabolic Research, Central Regulation of Metabolism, Faculty for Health and Medical Sciences, University of Copenhagen, Denmark; Max Planck Institute for Metabolism Research, Synaptic Transmission in Energy Homeostasis, Cologne, Germany; Center for Physical Activity Research, Rigshospitalet, University of Copenhagen, Denmark; Max Planck Institute for Neurobiology of Behavior, Behavior and Brain Organization, Bonn, Germany. Electronic address: diba.borgmann@sf.mpg.de. FAU - Rausch, Dylan M AU - Rausch DM AD - Novo Nordisk Foundation Center for Basic Metabolic Research, Central Regulation of Metabolism, Faculty for Health and Medical Sciences, University of Copenhagen, Denmark; Novo Nordisk A/S, Copenhagen, Denmark. FAU - Lundh, Sofia AU - Lundh S AD - Novo Nordisk A/S, Copenhagen, Denmark. FAU - Hassan, Shad AU - Hassan S AD - Novo Nordisk Foundation Center for Basic Metabolic Research, Central Regulation of Metabolism, Faculty for Health and Medical Sciences, University of Copenhagen, Denmark. FAU - Tokgoz, Cansu AU - Tokgoz C AD - Max Planck Institute for Metabolism Research, Synaptic Transmission in Energy Homeostasis, Cologne, Germany. FAU - Glatzel, Annika AU - Glatzel A AD - Max Planck Institute for Metabolism Research, Multimodal Imaging, Cologne, Germany. FAU - Cabot, Leonie AU - Cabot L AD - Max Planck Institute for Metabolism Research, Synaptic Transmission in Energy Homeostasis, Cologne, Germany. FAU - Ten Voorde, Malou Cajsa AU - Ten Voorde MC AD - Novo Nordisk Foundation Center for Basic Metabolic Research, Central Regulation of Metabolism, Faculty for Health and Medical Sciences, University of Copenhagen, Denmark. FAU - Baech-Laursen, Cecilie AU - Baech-Laursen C AD - Center for Physical Activity Research, Rigshospitalet, University of Copenhagen, Denmark. FAU - Sanchis Tortosa, Paula AU - Sanchis Tortosa P AD - Center for Physical Activity Research, Rigshospitalet, University of Copenhagen, Denmark. FAU - Brandt, Claus AU - Brandt C AD - Center for Physical Activity Research, Rigshospitalet, University of Copenhagen, Denmark; Novo Nordisk A/S, Copenhagen, Denmark. FAU - Backes, Heiko AU - Backes H AD - Max Planck Institute for Metabolism Research, Multimodal Imaging, Cologne, Germany. FAU - Pers, Tune H AU - Pers TH AD - Novo Nordisk Foundation Center for Basic Metabolic Research, Central Regulation of Metabolism, Faculty for Health and Medical Sciences, University of Copenhagen, Denmark. FAU - Fenselau, Henning AU - Fenselau H AD - Max Planck Institute for Metabolism Research, Synaptic Transmission in Energy Homeostasis, Cologne, Germany; Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases (CECAD), University of Cologne, Joseph-Stelzmann-Str. 26, 50931 Cologne, Germany; Policlinic for Endocrinology, Diabetes, and Preventive Medicine, University Hospital Cologne, Kerpener Str. 26, 50924 Cologne, Germany. Electronic address: henning.fenselau@sf.mpg.de. LA - eng PT - Journal Article DEP - 20261028 PL - Germany TA - Mol Metab JT - Molecular metabolism JID - 101605730 SB - IM OTO - NOTNLM OT - Brainstem OT - Energy expenditure OT - Exercise OT - Feeding behavior OT - Fibcd1 OT - Gut-brain-axis EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/08/14 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S2212-8778(26)00147-X [pii] AID - 10.1016/j.molmet.2026.102463 [doi] PST - aheadofprint SO - Mol Metab. 2026 Oct 28:102463. doi: 10.1016/j.molmet.2026.102463. PMID- 42951203 OWN - NLM STAT- Publisher LR - 20261028 IS - 1615-5947 (Electronic) IS - 0890-5096 (Linking) DP - 2026 Oct 28 TI - Functional Vulnerability and Long-Term Mortality in Adults With Ankle-Brachial Index-Defined Peripheral Artery Disease: A NHANES Cohort Study. LID - S0890-5096(26)00755-7 [pii] LID - 10.1016/j.avsg.2026.10.028 [doi] AB - OBJECTIVES: Functional limitations are common in peripheral artery disease (PAD), but the association of a brief self-reported functional-burden measure with objectively defined PAD and long-term mortality remains incompletely characterized. METHODS: We analyzed National Health and Nutrition Examination Survey 1999-2004 participants aged >=40 years with ankle-brachial index (ABI), physical functioning, and linked mortality data. PAD was defined as minimum ABI <=0.90. A NHANES-derived functional vulnerability index (NFVI; range 0-4) was constructed from difficulty walking a quarter mile, walking up ten steps, stooping/crouching/kneeling, and standing for long periods. NFVI was intended as a brief functional-burden measure rather than a diagnostic frailty instrument. Survey-weighted models evaluated PAD status, NFVI, and mortality, with validated modified cardiovascular health (mCVH) used for additional adjustment. RESULTS: The PAD NFVI cohort included 462 participants and 375 deaths. PAD was associated with high NFVI after adjustment for sociodemographic factors and mCVH (OR 1.748, 95% CI 1.218-2.510; P=0.005). In PAD participants, each 1-point higher NFVI was associated with all-cause mortality after adjustment for sociodemographic factors and minimum ABI (HR 1.095, 95% CI 1.017-1.179; P=0.017). After additional mCVH adjustment, the continuous association was attenuated (HR 1.075, 95% CI 0.993-1.165; P=0.075), whereas high NFVI remained associated with mortality (HR 1.346, 95% CI 1.026-1.767; P=0.032). Complete-case selection was substantial (462/647 PAD participants), with marked differences between included and excluded participants, particularly in age, potentially limiting generalizability. CONCLUSIONS: Functional vulnerability was more common among adults with ABI-defined PAD and was associated with long-term all-cause mortality. These findings demonstrate an epidemiologic association rather than the clinical utility, validation, or incremental prognostic value of NFVI and do not establish a clinically meaningful NFVI mortality threshold. Prospective PAD-specific validation and comparison with established frailty measures are required before clinical use. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Wang, Mingxiu AU - Wang M AD - Affiliation: Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221002, P.R. China. FAU - Wang, Yong AU - Wang Y AD - Affiliation: Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221002, P.R. China. Electronic address: mumei20071115@163.com. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Ann Vasc Surg JT - Annals of vascular surgery JID - 8703941 SB - IM OTO - NOTNLM OT - NHANES OT - ankle-brachial index OT - functional status OT - mobility limitation OT - mortality OT - peripheral artery disease EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/06/03 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S0890-5096(26)00755-7 [pii] AID - 10.1016/j.avsg.2026.10.028 [doi] PST - aheadofprint SO - Ann Vasc Surg. 2026 Oct 28:S0890-5096(26)00755-7. doi: 10.1016/j.avsg.2026.10.028. PMID- 42951194 OWN - NLM STAT- Publisher LR - 20261028 IS - 1615-5947 (Electronic) IS - 0890-5096 (Linking) DP - 2026 Oct 28 TI - Five-Year Outcomes of Covered Stent-Graft Repair for Traumatic and Iatrogenic Femoral Artery Injuries. LID - S0890-5096(26)00760-0 [pii] LID - 10.1016/j.avsg.2026.10.033 [doi] AB - BACKGROUND: Endovascular repair with covered stent-grafts offers a prompt and effective strategy to manage lower-extremity arterial trauma and catheterization-related iatrogenic vascular complications. However, long-term durability and late complication profiles of covered stent-grafts in the femoral segments remain poorly defined. The aim of the study was to evaluate the long-term outcomes of covered stent-grafting for traumatic and iatrogenic injuries of the common, superficial, and deep femoral arteries over a 5-year follow-up period. METHODS: In this single-center retrospective study, 58 consecutive patients (40 males [69.0%]; mean age: 57 +/- 13.6 years) who underwent endovascular repair for femoral arterial injuries between August 2011 and August 2021 were analyzed. The primary outcome was the 5-year primary patency rate. Secondary outcomes included primary-assisted and secondary patency rates, as well as subgroup comparisons of patency based on stent-graft type, lesion type, and injury type. RESULTS: Etiologies included iatrogenic injury in 36 patients (62.1%) and traumatic injury in 22 patients (37.9%). Technical success was achieved in 100% of cases. The overall 5-year Kaplan-Meier estimates for primary, primary-assisted, and secondary patency rates were 72.4%, 91.4%, and 94.8%, respectively, with a mean primary patency duration of 52 months. Log-rank tests demonstrated no statistically significant differences in primary patency rates across stent-graft types (p = 0.942), lesion types (p = 0.751), or injury types (p = 0.864). CONCLUSION: In appropriately selected patients, covered stent-graft repair was associated with high technical success, low observed complication rates, and favorable long-term patency for traumatic and iatrogenic femoral arterial injuries. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Ates, Omer Faruk AU - Ates OF AD - Istanbul Atlas University, Faculty of Medicine, Department of Radiology, Istanbul, Turkiye. Electronic address: taydasonur@gmail.com. FAU - Taydas, Onur AU - Taydas O AD - Sakarya University, Faculty of Medicine, Department of Radiology, Sakarya, Turkiye. FAU - Canyigit, Murat AU - Canyigit M AD - Ankara Yildirim Beyazit University, Ankara Bilkent City Hospital, Department of Radiology, Ankara, Turkiye. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Ann Vasc Surg JT - Annals of vascular surgery JID - 8703941 SB - IM OTO - NOTNLM OT - arteriovenous fistula OT - covered stent OT - endovascular repair OT - pseudoaneurysm OT - vascular trauma COIS- Conflict of interest The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/09/29 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S0890-5096(26)00760-0 [pii] AID - 10.1016/j.avsg.2026.10.033 [doi] PST - aheadofprint SO - Ann Vasc Surg. 2026 Oct 28:S0890-5096(26)00760-0. doi: 10.1016/j.avsg.2026.10.033. PMID- 42951193 OWN - NLM STAT- Publisher LR - 20261028 IS - 1615-5947 (Electronic) IS - 0890-5096 (Linking) DP - 2026 Oct 28 TI - Brachial Plexus Injuries from Overhead Arm Positioning During F/BEVAR: A Case Series and Call for Standardized Technique. LID - S0890-5096(26)00749-1 [pii] LID - 10.1016/j.avsg.2026.10.022 [doi] AB - OBJECTIVE: Overhead arm positioning during fenestrated and branched endovascular aortic repair (F/BEVAR) may improve imaging quality and reduce procedural radiation dose. Elevated arm positioning during general anesthesia, however, may result in risk for nerve symptoms. The aim of this study was to assess the occurrence of upper extremity symptoms (UES) after overhead arm positioning during F/BEVAR. DESIGN: Single-center, retrospective, cohort. METHODS: All consecutive patients undergoing F/BEVAR performed with bilateral overhead arm positioning at *our* Hospital between 2023 and 2025 were included. Neutral head position, padded overhead support, and abduction limited to 180 minutes. Procedural technical success was 98.5%, and in-hospital mortality 1.5%. CONCLUSIONS: Bilateral overhead arm positioning during F/BEVAR was associated with a measurable incidence of UES. Although most symptoms were transient and resolved without intervention, clinically significant BPI occurred in a small proportion of patients despite standardized positioning. These findings support cautious and selective use of prolonged overhead arm positioning and warrant prospective evaluation of safe positioning duration and patient-related susceptibility. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Inc. All rights reserved. FAU - Bayona, Juan Gabriel AU - Bayona JG AD - Department of Surgical Sciences, Section of vascular surgery, Uppsala University, Uppsala, Sweden; Department of Vascular Surgery, Clinica Medellin, Medellin, Colombia. FAU - Hautala, Laura AU - Hautala L AD - Department of Diagnostics and Intervention, Surgery, Umea University, Umea, Sweden. FAU - Prendes, Carlota F AU - Prendes CF AD - Department of Surgical Sciences, Section of vascular surgery, Uppsala University, Uppsala, Sweden. FAU - Kuzniar, Marek AU - Kuzniar M AD - Department of Surgical Sciences, Section of vascular surgery, Uppsala University, Uppsala, Sweden. FAU - Wanhainen, Anders AU - Wanhainen A AD - Department of Surgical Sciences, Section of vascular surgery, Uppsala University, Uppsala, Sweden; Department of Diagnostics and Intervention, Surgery, Umea University, Umea, Sweden. FAU - Mani, Kevin AU - Mani K AD - Department of Surgical Sciences, Section of vascular surgery, Uppsala University, Uppsala, Sweden. Electronic address: kevin.mani@uu.se. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Ann Vasc Surg JT - Annals of vascular surgery JID - 8703941 SB - IM OTO - NOTNLM OT - brachial plexus injury OT - endovascular aortic repair OT - overhead arm support OT - patient positioning OT - radiation reduction EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/08/17 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S0890-5096(26)00749-1 [pii] AID - 10.1016/j.avsg.2026.10.022 [doi] PST - aheadofprint SO - Ann Vasc Surg. 2026 Oct 28:S0890-5096(26)00749-1. doi: 10.1016/j.avsg.2026.10.022. PMID- 42951188 OWN - NLM STAT- Publisher LR - 20261028 IS - 1615-5947 (Electronic) IS - 0890-5096 (Linking) DP - 2026 Oct 28 TI - Outcomes Following Celiac Plexus Neurectomy in Patients With Median Arcuate Ligament Syndrome: A Retrospective Survey Study. LID - S0890-5096(26)00750-8 [pii] LID - 10.1016/j.avsg.2026.10.023 [doi] AB - OBJECTIVE: To characterize patient-reported outcomes following open median arcuate ligament (MAL) resection with celiac plexus neurectomy, and identify preoperative factors associated with postoperative pain relief, recurrence, and symptom improvement. METHODS: Retrospective survey study at a single tertiary hospital. Patients undergoing open MAL resection with celiac plexus neurectomy between January 2019 and March 2022 were contacted; 224 of 503 (44.5%) completed a 19-item electronic questionnaire assessing initial pain relief, pain recurrence, and symptom improvement. Seven preoperative predictors including sex, age, pain duration, symptom burden, nutritional support, celiac plexus block (CPB) response, and prior MALS surgery were evaluated using Firth-penalized logistic regression, interval-censored survival analysis, and multivariable linear regression, with Bonferroni correction applied to exploratory predictors. RESULTS: The cohort was 89.1% female with median age of 21 [17-34] years. Initial relief was reported by 211/224 (94.2%); of these, 50 (23.7%) experienced recurrence, typically within one to six months. Median symptom improvement score was 6 [4-7]. Complete preoperative CPB response predicted reduced recurrence hazard (HR 0.48 [0.25-0.91]; p=0.024), greater symptom improvement (beta 2.02 [0.99-3.05]; p<0.001), and higher one-year freedom from recurrence (OR 2.41 [1.11-5.21]; p=0.026); no other predictor was significant. CONCLUSIONS: Open MAL resection with celiac plexus neurectomy yielded high rates of initial pain relief and durable benefit. Complete preoperative CPB response was the only independent predictor of reduced recurrence and greater symptom improvement, supporting its use in patient selection and counseling. Revision surgery without prior neurectomy achieved outcomes comparable to primary surgery, supporting re-operation in appropriate patients. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Inc. All rights reserved. FAU - Shung, Artemis AU - Shung A AD - Department of Research and Discovery, Stamford Health, One Hospital Plaza, Stamford, Connecticut 06902, USA,. Electronic address: ashung@stamhealth.org. FAU - Rose, Suzanne J AU - Rose SJ AD - Department of Research and Discovery, Stamford Health, One Hospital Plaza, Stamford, Connecticut 06902, USA,. Electronic address: srose@stamhealth.org. FAU - Hsu, Richard AU - Hsu R AD - Department of Surgery, Stamford Hospital, One Hospital Plaza, Stamford, Connecticut 06902, USA. Electronic address: rhsu1@stamhealth.org. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Ann Vasc Surg JT - Annals of vascular surgery JID - 8703941 SB - IM OTO - NOTNLM OT - Celiac plexus OT - Celiac plexus block OT - MALS OT - Median arcuate ligament syndrome OT - Patient Reported Outcome Measures EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/07/28 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S0890-5096(26)00750-8 [pii] AID - 10.1016/j.avsg.2026.10.023 [doi] PST - aheadofprint SO - Ann Vasc Surg. 2026 Oct 28:S0890-5096(26)00750-8. doi: 10.1016/j.avsg.2026.10.023. PMID- 42951186 OWN - NLM STAT- Publisher LR - 20261028 IS - 1615-5947 (Electronic) IS - 0890-5096 (Linking) DP - 2026 Oct 28 TI - Long-Term Patency and Clinical Outcomes After Retrograde Mesenteric Bypass for Chronic Mesenteric Ischemia. LID - S0890-5096(26)00751-X [pii] LID - 10.1016/j.avsg.2026.10.024 [doi] AB - OBJECTIVES: Chronic mesenteric ischemia (CMI) is an uncommon yet clinically significant condition resulting from insufficient blood flow to the gastrointestinal tract, frequently leading to severe symptoms and increased morbidity. Although endovascular therapy has become the initial treatment strategy in many centres, open surgical reconstruction remains an important option for selected patients. This study evaluated long-term graft patency and clinical outcomes after retrograde mesenteric bypass. METHODS: We evaluated 34 patients treated with retrograde mesenteric bypass for CMI between 2009 and 2024. Patency rates were measured with Kaplan-Meier analysis. Thrombosis was classified as early (365 days). RESULTS: Superior mesenteric artery bypass was performed in 33 patients (97.1%), celiac bypass in 25 (73.5%), and dual-vessel reconstruction in 24 (70.6%). Median follow-up was 2737 days (IQR, 1760-4353), approximately 7.5 years. No early thrombosis occurred. Two mid-term and three late occlusions were observed. Primary patency rates were 93.3% at 1 and 3 years, 89.4% at 5 years, and 85.0% at 10 years. Clinical symptoms resolved after revascularisation in all patients surviving the perioperative period, and no recurrent symptoms were documented during follow-up. No patient required reintervention. CONCLUSIONS: Retrograde mesenteric bypass was associated with stable long-term patency, sustained symptom resolution, and low perioperative mortality. It remains a durable open surgical option for chronic mesenteric ischemia, particularly when endovascular revascularisation is not feasible or is unlikely to provide durable results. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Sezen, Arda AU - Sezen A AD - Department of Cardiovascular Surgery, Faculty of Medicine, Ege University, Izmir, Turkey. Electronic address: arda.sezen.13@gmail.com. FAU - Kapkin, Aysen Yaprak AU - Kapkin AY AD - Department of Cardiovascular Surgery, Faculty of Medicine, Ege University, Izmir, Turkey. FAU - Oguz, Emrah AU - Oguz E AD - Department of Cardiovascular Surgery, Faculty of Medicine, Ege University, Izmir, Turkey. FAU - Posacioglu, Hakan AU - Posacioglu H AD - Department of Cardiovascular Surgery, Faculty of Medicine, Ege University, Izmir, Turkey. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Ann Vasc Surg JT - Annals of vascular surgery JID - 8703941 SB - IM OTO - NOTNLM OT - Blood Vessel Prosthesis OT - Mesenteric Arteries OT - Mesenteric Ischemia OT - Revascularization OT - Vascular Patency COIS- Conflict of interest None declared. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/08/16 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S0890-5096(26)00751-X [pii] AID - 10.1016/j.avsg.2026.10.024 [doi] PST - aheadofprint SO - Ann Vasc Surg. 2026 Oct 28:S0890-5096(26)00751-X. doi: 10.1016/j.avsg.2026.10.024. PMID- 42951180 OWN - NLM STAT- Publisher LR - 20261028 IS - 1615-5947 (Electronic) IS - 0890-5096 (Linking) DP - 2026 Oct 28 TI - Early and midterm outcomes of the Gore Excluder Iliac Branch Endoprosthesis within and outside the instructions for use for aortoiliac and iliac artery aneurysms. LID - S0890-5096(26)00747-8 [pii] LID - 10.1016/j.avsg.2026.10.020 [doi] AB - OBJECTIVE: To evaluate perioperative and midterm outcomes of endovascular aneurysm repair using the Gore Excluder Iliac Branch Endoprosthesis (IBE) for aortoiliac and iliac artery aneurysms treated within and outside the instructions for use (IFU). METHODS: This single-center retrospective cohort study included patients who underwent IBE implantation for non-ruptured aortoiliac or iliac artery aneurysms between April 2017 and December 2022. Outcomes were analyzed at the patient, procedure, or IBE-treated-side level, as appropriate. The primary endpoints were technical success, IBE-related complications, and reinterventions. Secondary endpoints included internal iliac artery (IIA) branch patency, aneurysm sac status, and freedom from reintervention or aneurysm-related death. RESULTS: A total of 56 unique patients underwent 58 procedures involving 73 IBE-treated sides; 24 sides were within-IFU and 49 were outside-IFU. Technical success was achieved in 100% of within-IFU procedures and 95.0% of outside-IFU procedures. At 36 months, freedom from reintervention was 79.1% and 94.0% in the within-IFU and outside-IFU groups, respectively (P = .24), and IIA branch patency was 88.8% and 85.5%, respectively. No significant between-group difference in IIA branch patency was detected after accounting for within-patient clustering (hazard ratio, 1.53; 95% confidence interval, 0.28-8.35; P = .62). Seven IIA branch occlusions occurred during follow-up, none of which was associated with buttock claudication. In exploratory univariable analysis, type II endoleak was associated with CIA aneurysm sac growth (odds ratio, 7.86; 95% confidence interval, 1.08-57.0; P = .04). CONCLUSIONS: IBE use outside the IFU was feasible in carefully selected patients. No significant differences in midterm clinical outcomes were detected between the within-IFU and outside-IFU groups. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Hara, Masayuki AU - Hara M AD - Division of Vascular Surgery, Department of Surgery, The Jikei University School of Medicine, 3-25-8, Nishi-shimbashi, Minato-ku, Tokyo, 105-8461, Japan. Electronic address: haharappy@hotmail.com. FAU - Kasa, Kentaro AU - Kasa K AD - Division of Vascular Surgery, Department of Surgery, The Jikei University School of Medicine, 3-25-8, Nishi-shimbashi, Minato-ku, Tokyo, 105-8461, Japan. Electronic address: kasa@jikei.ac.jp. FAU - Ohki, Takao AU - Ohki T AD - Division of Vascular Surgery, Department of Surgery, The Jikei University School of Medicine, 3-25-8, Nishi-shimbashi, Minato-ku, Tokyo, 105-8461, Japan. Electronic address: takohki@msn.com. FAU - Shukuzawa, Kota AU - Shukuzawa K AD - Division of Vascular Surgery, Department of Surgery, The Jikei University School of Medicine, 3-25-8, Nishi-shimbashi, Minato-ku, Tokyo, 105-8461, Japan. Electronic address: kota.shukuzawa@gmail.com. FAU - Fukushima, Soichiro AU - Fukushima S AD - Division of Vascular Surgery, Department of Surgery, The Jikei University School of Medicine, 3-25-8, Nishi-shimbashi, Minato-ku, Tokyo, 105-8461, Japan. Electronic address: sfuku713@gmail.com. FAU - Ozawa, Hirotsugu AU - Ozawa H AD - Division of Vascular Surgery, Department of Surgery, The Jikei University School of Medicine, 3-25-8, Nishi-shimbashi, Minato-ku, Tokyo, 105-8461, Japan. Electronic address: hirotsugu_ozawa@yahoo.co.jp. FAU - Omori, Makiko AU - Omori M AD - Division of Vascular Surgery, Department of Surgery, The Jikei University School of Medicine, 3-25-8, Nishi-shimbashi, Minato-ku, Tokyo, 105-8461, Japan. Electronic address: o.makibawamidori@gmail.com. FAU - Shirouzu, Miyo AU - Shirouzu M AD - Division of Vascular Surgery, Department of Surgery, The Jikei University School of Medicine, 3-25-8, Nishi-shimbashi, Minato-ku, Tokyo, 105-8461, Japan. Electronic address: miyoshirouzu@gmail.com. FAU - Nakagawa, Hikaru AU - Nakagawa H AD - Division of Vascular Surgery, Department of Surgery, The Jikei University School of Medicine, 3-25-8, Nishi-shimbashi, Minato-ku, Tokyo, 105-8461, Japan. Electronic address: hnakagawa5678@gmail.com. FAU - Nishide, Ryo AU - Nishide R AD - Division of Vascular Surgery, Department of Surgery, The Jikei University School of Medicine, 3-25-8, Nishi-shimbashi, Minato-ku, Tokyo, 105-8461, Japan. Electronic address: ryounishide@gmail.com. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Ann Vasc Surg JT - Annals of vascular surgery JID - 8703941 SB - IM OTO - NOTNLM OT - Endovascular aneurysm repair OT - iliac artery aneurysm OT - iliac branch endoprosthesis OT - instructions for use OT - internal iliac artery preservation EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S0890-5096(26)00747-8 [pii] AID - 10.1016/j.avsg.2026.10.020 [doi] PST - aheadofprint SO - Ann Vasc Surg. 2026 Oct 28:S0890-5096(26)00747-8. doi: 10.1016/j.avsg.2026.10.020. PMID- 42951179 OWN - NLM STAT- Publisher LR - 20261028 IS - 1553-4669 (Electronic) IS - 1553-4650 (Linking) DP - 2026 Oct 28 TI - Author's Reply. LID - S1553-4650(26)01007-1 [pii] LID - 10.1016/j.jmig.2026.10.015 [doi] FAU - Servidoni, Ana Clara Pimenta AU - Servidoni ACP AD - Gynecology Division, Beneficencia Portuguesa de Sao Paulo, Sao Paulo, SP, Brazil; Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of Medicine, New Haven, Connecticut, USA; Faculdade Santa Marcelina, Sao Paulo, Brazil. FAU - Tristao, Luca Schiliro AU - Tristao LS AD - Department of Gynecology and Obstetrics, Centro Universitario Faculdade de Medicina do ABC, Santo Andre, Brazil. FAU - Lemos, Maria Julia AU - Lemos MJ AD - Department of Women's Health, Kurah Women's Health Institute, Rio de Janeiro, Brazil. FAU - Surmann, Hanna AU - Surmann H AD - Department of Gynecology and Obstetrics, University of Muenster, Muenster, Germany. FAU - Curi-Pesantes, Berenice AU - Curi-Pesantes B AD - Mailman School of Public Health, Columbia University, New York, USA; Department of Obstetrics and Gynecology, Wellspan Health, Pennsylvania, USA. FAU - Perroni, Pedro Mussi AU - Perroni PM AD - Faculdade de Medicina de Ribeirao Preto da Universidade de Sao Paulo (FMRP-USP), Sao Paulo, SP, Brazil. FAU - Mello, Pedro Lucas Pinheiro AU - Mello PLP AD - Faculdade Santa Marcelina, Sao Paulo, Brazil. FAU - Nakayama, Camila M Aihara AU - Nakayama CMA AD - School of Medicine, University of Mogi das Cruzes, Mogi das Cruzes, SP, Brazil; Hospital of the University of Sao Paulo School of Medicine (HCFMUSP), Sao Paulo, Sao Paulo, Brazil. FAU - Lopes, Marcio Delascio AU - Lopes MD AD - Faculdade Santa Marcelina, Sao Paulo, Brazil. FAU - Ribeiro, Paulo A AU - Ribeiro PA AD - Irmandade Santa Casa de Misericordia do Estado de Sao Paulo, Obstetrics and Gynecology Department, Sao Paulo, Brazil. FAU - Taylor, Hugh S AU - Taylor HS AD - Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of Medicine, New Haven, Connecticut, USA. FAU - Abrao, Mauricio S AU - Abrao MS AD - Gynecology Division, Beneficencia Portuguesa de Sao Paulo, Sao Paulo, SP, Brazil; Gynecology Division, Obstetrics and Gynecology Department, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo, SP, Brazil. Electronic address: msabrao@mac.com. LA - eng PT - Letter DEP - 20261028 PL - United States TA - J Minim Invasive Gynecol JT - Journal of minimally invasive gynecology JID - 101235322 SB - IM COIS- Declaration of competing interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/10/20 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S1553-4650(26)01007-1 [pii] AID - 10.1016/j.jmig.2026.10.015 [doi] PST - aheadofprint SO - J Minim Invasive Gynecol. 2026 Oct 28:S1553-4650(26)01007-1. doi: 10.1016/j.jmig.2026.10.015. PMID- 42951178 OWN - NLM STAT- Publisher LR - 20261028 IS - 1531-5037 (Electronic) IS - 0022-3468 (Linking) DP - 2026 Oct 28 TI - Comparison of Surgical Approaches for Hirschsprung's Disease: A Network Meta-Analysis. PG - 163568 LID - S0022-3468(26)00650-0 [pii] LID - 10.1016/j.jpedsurg.2026.163568 [doi] AB - PURPOSE: Hirschsprung's disease can be treated using multiple procedures, including Soave, Swenson, and Duhamel techniques, performed via open, laparoscopic, transanal, or robotic approaches. However, the optimal surgical approaches remain controversial, particularly regarding perioperative efficiency and long-term bowel function. We performed a network meta-analysis to compare these approaches. MATERIALS AND METHODS: A comprehensive literature search was conducted according to PRISMA guidelines across electronic databases, including PubMed, Embase, and the Cochrane Library, from inception until the last update on October 31, 2025. Nine techniques (open, laparoscopic, transanal Soave and Swenson; open, laparoscopic Duhamel; and robotic-assisted procedures) were included. Primary outcomes were operative time, length of stay, and blood loss; secondary outcomes were postoperative Hirschsprung-associated enterocolitis (HAEC), constipation, and fecal incontinence. A random-effects frequentist network meta-analysis was conducted using MetaInsight to obtain the ranking probabilities. RESULTS: Twenty-eight studies, including 1,911 patients, were included. For perioperative outcomes, Transanal Swenson (TSw) had the shortest operative time, followed by Transanal Soave (TS) and Laparoscopic Swenson (LSw), whereas Open/ Laparoscopic Duhamel and robotic-assisted procedures required longer times. LSw ranked best for length of stay, and Robotic-assisted surgery was associated with shorter hospitalization compared with most open techniques. Robotic-assisted surgery showed the lowest intraoperative blood loss and was statistically superior to the other approaches. No significant differences were observed in postoperative HAEC, constipation, or incontinence; bowel function outcomes were comparable across techniques. CONCLUSION: This is the first network meta-analysis to comprehensively compare all major surgical approaches, suggesting that the choice of surgical approach for Hirschsprung's disease mainly affects perioperative efficiency rather than long-term bowel function. Minimally invasive and transanal procedures offer advantages in operative time and hospitalization, and robotic surgery minimizes blood loss. No significant differences were identified in postoperative HAEC, constipation, or incontinence. These findings may guide surgical planning in consideration of perioperative care. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Ku, Hsiao-Chien AU - Ku HC AD - Division of Pediatric Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan. FAU - Wei, Chin-Hung AU - Wei CH AD - Division of Pediatric Surgery, Department of Surgery, Shuang-Ho Hospital, New Taipei City, Taiwan; Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan. FAU - Huang, Yan-Jiun AU - Huang YJ AD - Division of Colorectal Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan; Division of General Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan; Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan. FAU - Huang, Fu-Huan AU - Huang FH AD - Division of Pediatric Surgery, Department of Surgery, Taipei Medical University Hospital, Taipei, Taiwan; Department of Surgery, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan. Electronic address: 191009@h.tmu.edu.tw. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Pediatr Surg JT - Journal of pediatric surgery JID - 0052631 SB - IM OTO - NOTNLM OT - Duhamel OT - Hirschsprung's disease OT - Soave OT - Swenson OT - network meta-analysis OT - pull-through procedure OT - robotic surgery OT - transanal endorectal pull-through COIS- Conflict of interest All authors declare no conflicts of interest. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/03/01 00:00 [received] PHST- 2026/06/01 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S0022-3468(26)00650-0 [pii] AID - 10.1016/j.jpedsurg.2026.163568 [doi] PST - aheadofprint SO - J Pediatr Surg. 2026 Oct 28:163568. doi: 10.1016/j.jpedsurg.2026.163568. PMID- 42951173 OWN - NLM STAT- Publisher LR - 20261028 IS - 1096-0953 (Electronic) IS - 0013-9351 (Linking) DP - 2026 Oct 28 TI - A Dual-Function Hydrogel Platform for Cu(II)/Fe(III) Remediation and Controlled Nutrient Release. PG - 126052 LID - S0013-9351(26)02387-X [pii] LID - 10.1016/j.envres.2026.126052 [doi] AB - The development of multifunctional materials that address multiple environmental challenges simultaneously represents a paradigm shift in sustainable water and soil management. This study presents a dual functional hydrogel consisting of urea-biochar encapsulated crosslinked hydrogel (U-BECA) designed for Cu(II)/Fe(III) removal and controlled nutrient release. The hydrogel was synthesized through calcium ion crosslinking of sodium alginate incorporating biochar and urea, characterized using FTIR, SEM, EDS, DMA, XRD and evaluated under single and binary metal ion systems. Results demonstrated effective adsorption with selectivity order Fe(3+) > Cu(2+). The urea-loading strategy also exhibited sustained urea release over extended periods while maintaining metal ion adsorption capacity, offering integrated pollution remediation and nutrient delivery. The Fe(3+) and Cu(2+) adsorption respectively followed Langmuir and Sips model with theoretical adsorption capacities of 131.38 mg/g for Fe(3+) and 95.63 mg/g for Cu(2+). Dynamic mechanical analysis (DMA) confirmed the hydrogel's structural integrity, exhibiting a storage modulus of 319 kPa and no gel-to-sol transition, indicative of a mechanically stable and highly elastic network. SEM-EDS analysis revealed a dense, Ca(2+)-crosslinked microstructure that became rougher and enriched with particulate deposits following Cu(2+) and Fe(3+) adsorption. The persistence of characteristic metal peaks in the EDS spectra further confirmed successful metal uptake and retention within the hydrogel matrix. The effect of pH on urea release was evaluated, revealing pH-responsive release behavior. The Korsmeyer-Peppas model accurately described the release kinetics at pH 4 (n = 0.347), indicating a Fickian diffusion-controlled mechanism. The hydrogel also retained substantial adsorption capacity for both Cu(2+) and Fe(3+) over three consecutive adsorption-desorption cycles. These findings highlight hydrogel as a promising multifunctional material for integrated metal ion remediation and controlled nutrient delivery. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Umar, Muhammad AU - Umar M AD - University of Doha for Science and Technology, 24449 Arab League St, Doha, Qatar. FAU - Udayakumar, Sruthi AU - Udayakumar S AD - University of Doha for Science and Technology, 24449 Arab League St, Doha, Qatar. FAU - Evans, Eric A AU - Evans EA AD - Arizona State University, 660 S. College Avenue, Tempe, AZ, 85287, USA. FAU - Fini, Elham H AU - Fini EH AD - Arizona State University, 660 S. College Avenue, Tempe, AZ, 85287, USA. FAU - Akbarzadeh, Vajiheh AU - Akbarzadeh V AD - University of Doha for Science and Technology, 24449 Arab League St, Doha, Qatar. Electronic address: Vajiheh.Akbarzadeh@udst.edu.qa. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Environ Res JT - Environmental research JID - 0147621 SB - IM OTO - NOTNLM OT - Circular agriculture OT - Controlled nutrient release OT - Fickian diffusion OT - Hydrogel OT - Metal ion adsorption OT - Urea-loaded hydrogel OT - Water remediation COIS- Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/08/12 00:00 [received] PHST- 2026/10/22 00:00 [revised] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S0013-9351(26)02387-X [pii] AID - 10.1016/j.envres.2026.126052 [doi] PST - aheadofprint SO - Environ Res. 2026 Oct 28:126052. doi: 10.1016/j.envres.2026.126052. PMID- 42951172 OWN - NLM STAT- Publisher LR - 20261028 IS - 1096-0953 (Electronic) IS - 0013-9351 (Linking) DP - 2026 Oct 28 TI - Associations of multiple ambient air pollutants and sleep apnea, sleep architecture and nocturnal oxygenation in adult patients with or without obstructive sleep apnea (OSA). PG - 126044 LID - S0013-9351(26)02379-0 [pii] LID - 10.1016/j.envres.2026.126044 [doi] AB - Obstructive sleep apnea (OSA) affects an estimated 936 million adults worldwide, yet how environmental exposures relate to sleep architecture and nocturnal oxygenation remains unclear. We conducted a retrospective polysomnography-based study of 4049 adults in Shenzhen, China, to examine the relationships of ambient air pollutants, PM(2.5) inorganic components, and meteorological factors with OSA severity, sleep architecture, and nocturnal oxygenation. Lag 0-180 days was selected post hoc as the main reporting window. Within this window, PM(2.5), PM(10), NO(2), SO(2), CO, and diurnal temperature range (DTR) were all linked to greater OSA severity. Multiple pollutants were associated with lighter sleep; for example, PM(2.5) and PM(10) were associated with 1.71%TST and 1.32%TST increases in N1 sleep and 1.51%TST and 1.32%TST decreases in N3 sleep, respectively. O(3) and DTR showed the clearest adverse relationships with nocturnal oxygenation and sleep continuity. Non-linear analyses showed increasing exposure-response associations for PM(2.5), SO(2), and DTR, but a U-shaped association for O(3). Interaction and mixture analyses further indicated meteorological modification and more consistent mixture relationships with altered sleep architecture than with categorical OSA severity. These findings highlight the importance of broader polysomnographic dimensions, cumulative exposure windows, and co-exposure patterns in future research. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Wang, Xia-Yuhui AU - Wang XY AD - Department of Public Health and Preventive Medicine, School of Medicine, Jinan University, Guangzhou 510632, China. FAU - Wang, Suhan AU - Wang S AD - Medical Research Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510080, China; Sleep Center, Department of Pulmonary and Critical Care Medicine, Guangdong Provincial Geriatrics Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou 510080, China. FAU - Tan, Xinyu AU - Tan X AD - Department of Public Health and Preventive Medicine, School of Medicine, Jinan University, Guangzhou 510632, China. FAU - Wu, Yu AU - Wu Y AD - Department of Public Health and Preventive Medicine, School of Medicine, Jinan University, Guangzhou 510632, China. FAU - Tang, Yuming AU - Tang Y AD - Department of Sleep Medicine, Institute of Respiratory Diseases, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen, Guangdong, China. FAU - Zhong, Siyi AU - Zhong S AD - Department of Public Health and Preventive Medicine, School of Medicine, Jinan University, Guangzhou 510632, China. FAU - Huang, Guangtong AU - Huang G AD - Department of Public Health and Preventive Medicine, School of Medicine, Jinan University, Guangzhou 510632, China. FAU - Fang, Jian AU - Fang J AD - Department of Public Health and Preventive Medicine, School of Medicine, Jinan University, Guangzhou 510632, China. FAU - Yang, Pan AU - Yang P AD - Department of Public Health and Preventive Medicine, School of Medicine, Jinan University, Guangzhou 510632, China; Guangdong Provincial Key Laboratory of Environmental Pollution and Health, Jinan University, Guangzhou 510632, China; Key Laboratory of Viral Pathogenesis & Infection Prevention and Control (Jinan University), Ministry of Education, Guangzhou 510632, China. Electronic address: yangpan419@163.com. FAU - Cheng, Hanrong AU - Cheng H AD - Department of Sleep Medicine, Institute of Respiratory Diseases, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, The First Affiliated Hospital of Southern University of Science and Technology, Shenzhen, Guangdong, China; Institute of Health Medicine, Southern University of Science and Technology, Shenzhen 518055, China. Electronic address: cheng.hanrong@szhospital.com. FAU - Chen, Gongbo AU - Chen G AD - Climate, Air Quality Research Unit, School of Public Health and Preventive Medicine, Monash University, Melbourne, Australia. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Environ Res JT - Environmental research JID - 0147621 SB - IM OTO - NOTNLM OT - Air pollution OT - Obstructive sleep apnea OT - Oxygen saturation OT - Sleep architecture COIS- Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/08/02 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S0013-9351(26)02379-0 [pii] AID - 10.1016/j.envres.2026.126044 [doi] PST - aheadofprint SO - Environ Res. 2026 Oct 28:126044. doi: 10.1016/j.envres.2026.126044. PMID- 42951166 OWN - NLM STAT- Publisher LR - 20261028 IS - 1096-0953 (Electronic) IS - 0013-9351 (Linking) DP - 2026 Oct 28 TI - Assessing sources of lead exposure in bearded vultures (Gypaetus barbatus) in the French Alps. PG - 126025 LID - S0013-9351(26)02360-1 [pii] LID - 10.1016/j.envres.2026.126025 [doi] AB - The reduction of mortality is essential for the conservation of long-lived threatened bird species such as the bearded vulture (Gypaetus barbatus) and poisoning due to the ingestion of lead (Pb) ammunition used in hunting is among its main threats. In this study, we non-invasively assessed Pb exposure in the bearded vulture population of the French Alps by analyzing segments of flight feathers. Our aim was to determine the incidence of abnormal Pb exposure and examine its relationship with physiological stress, reflected by corticosterone levels in feathers. We also evaluated the contribution of Pb ammunition and diet, including soil ingestion, to Pb exposure in these birds, and examined its relationship with the Pb isotopic signature observed in birds, including some birds of prey found dead in the study area. The sequential analysis of flight feather segments allowed us to establish a monthly incidence of abnormal lead exposure of 3.3%. Corticosterone concentrations in flight feathers were not affected by Pb exposure. Regarding the contribution of different Pb sources, prey and soil contributed 0.021 and 0.016 mg/kg bw per day, respectively, whereas Pb ammunition particles ingested by scavengers when consuming shot prey frequently exceeded 100 mg. The isotopic signature of Pb ammunition is highly variable among manufacturers, but the discriminant analysis made it possible to associate Pb in the tissues of poisoned birds with the ingestion of Pb ammunition. Although the incidence of Pb exposure in bearded vultures is lower than in other scavenger birds in Europe, their conservation status underscores the need to reduce avoidable causes of mortality. In particular, Pb poisoning could be reduced by replacing Pb-based ammunition with non-toxic alternatives. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Mateo, Rafael AU - Mateo R AD - Institute of Environmental Assessment and Water Research, IDAEA-CSIC, Jordi Girona 18-26, 08034 Barcelona; Institute for Game and Wildlife Research, IREC (CSIC-UCLM-JCCM), Rda. de Toledo 12, 13071 Ciudad Real, Spain. Electronic address: rafael.mateo@idaea.csic.es. FAU - Gimeno-Lozano, Lidia AU - Gimeno-Lozano L AD - Institute for Game and Wildlife Research, IREC (CSIC-UCLM-JCCM), Rda. de Toledo 12, 13071 Ciudad Real, Spain. FAU - Monclus, Laura AU - Monclus L AD - Department of Animal Health and Anatomy, Autonomous University of Barcelona, Bellaterra 08193, Spain; Norwegian Geotechnical Institute (NGI), Professor Brochs gate 2, Trondheim 7030, Norway. FAU - Jimenez-Moreno, Maria AU - Jimenez-Moreno M AD - Instituto de Ciencias Ambientales, Universidad de Castilla-La Mancha, Avda. Carlos III s/n, 45071 Toledo, Spain. FAU - Rodriguez Martin-Doimeadios, Rosa C AU - Rodriguez Martin-Doimeadios RC AD - Instituto de Ciencias Ambientales, Universidad de Castilla-La Mancha, Avda. Carlos III s/n, 45071 Toledo, Spain. FAU - Camarero, Pablo R AU - Camarero PR AD - Institute for Game and Wildlife Research, IREC (CSIC-UCLM-JCCM), Rda. de Toledo 12, 13071 Ciudad Real, Spain. FAU - Lopez-Bejar, Manel AU - Lopez-Bejar M AD - Department of Animal Health and Anatomy, Autonomous University of Barcelona, Bellaterra 08193, Spain. FAU - Champly, Ilka AU - Champly I AD - Asters, Conservatory of natural areas of Haute-Savoie, France. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Environ Res JT - Environmental research JID - 0147621 SB - IM OTO - NOTNLM OT - EU OT - Pb ammunition OT - birds OT - conservation OT - hunting OT - regulation COIS- Declaration of Competing Interest ☒ The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Ilka Champly reports financial support was provided by European Commission. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/06/09 00:00 [received] PHST- 2026/10/25 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S0013-9351(26)02360-1 [pii] AID - 10.1016/j.envres.2026.126025 [doi] PST - aheadofprint SO - Environ Res. 2026 Oct 28:126025. doi: 10.1016/j.envres.2026.126025. PMID- 42951162 OWN - NLM STAT- Publisher LR - 20261028 IS - 1872-6240 (Electronic) IS - 0006-8993 (Linking) DP - 2026 Oct 28 TI - Paclitaxel induces region-specific microglial remodeling associated with chronic behavioral deficits and modulated by electroacupuncture in rats. PG - 150620 LID - S0006-8993(26)00482-8 [pii] LID - 10.1016/j.brainres.2026.150620 [doi] AB - Chemotherapy-associated behavioral deficits, including cognitive impairment and depression, collectively termed chemobrain, often persist into the chronic phase after treatment, yet their underlying cellular mechanisms remain unclear. In particular, it remains unknown whether long-lasting behavioral deficits are associated with sustained neuroinflammatory responses or other forms of microglial alteration. We therefore examined whether chronic behavioral deficits were associated with region-specific microglial morphological alterations and inflammatory gene expression changes, and how EA intervention was associated with these alterations. Male SD rats were assigned to the Control, PTX, and PTX + EA groups. Cognitive and emotional behaviors were assessed using behavioral tests. Microglial morphology in the hippocampus across the dorsoventral axis and mPFC was analyzed using skeleton analysis, and inflammatory gene expression was evaluated by qPCR. PTX induced cognitive impairment and depressive-like behavior, accompanied by a selective reduction in microglial process number and length in the dentate gyrus of the dorsal hippocampus and the infralimbic cortex of the mPFC. Microglial morphology differed between the PTX and PTX + EA groups in both regions, although the difference in the dorsal dentate gyrus was limited to process number. No significant changes in the inflammatory genes examined were detected in the hippocampus or mPFC. Thus, PTX produced chronic behavioral deficits accompanied by selective microglial remodeling in the dorsal dentate gyrus and infralimbic cortex, but not in the ventral hippocampus, with no detectable changes in inflammatory gene expression. Behavioral outcomes and microglial morphology differed between the PTX and PTX + EA groups, indicating an association between EA intervention and region-specific microglial remodeling. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Hiraiwa, Shinya AU - Hiraiwa S AD - Department of Acupuncture and Moxibustion, Meiji University of Integrative Medicine, Nantan, Kyoto, Japan. FAU - Muramoto, Taiga AU - Muramoto T AD - Department of Systematic Anatomy and Neurobiology, The University of Osaka Graduate School of Dentistry, Suita, Osaka, Japan; Department of Anatomy, Meiji University of Integrative Medicine, Nantan, Kyoto, Japan. FAU - Umeda, Masahiro AU - Umeda M AD - Meiji University of Integrative Medicine, Nantan, Kyoto, Japan. FAU - Ebara, Satomi AU - Ebara S AD - Department of Anatomy, Meiji University of Integrative Medicine, Nantan, Kyoto, Japan. FAU - Fukuda, Fumihiko AU - Fukuda F AD - Department of Acupuncture and Moxibustion, Meiji University of Integrative Medicine, Nantan, Kyoto, Japan. Electronic address: f_fukuda@meiji-u.ac.jp. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Brain Res JT - Brain research JID - 0045503 SB - IM OTO - NOTNLM OT - Chemobrain OT - Electroacupuncture OT - Hippocampus OT - Medial prefrontal cortex OT - Microglia OT - Paclitaxel COIS- Declaration of competing interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/06/02 00:00 [received] PHST- 2026/08/26 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S0006-8993(26)00482-8 [pii] AID - 10.1016/j.brainres.2026.150620 [doi] PST - aheadofprint SO - Brain Res. 2026 Oct 28:150620. doi: 10.1016/j.brainres.2026.150620. PMID- 42951159 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-2763 (Electronic) IS - 1873-2763 (Linking) DP - 2026 Oct 28 TI - Automated laboratory detection of hypercalcemia: Diagnostic yield, clinical pathway and outcomes in a real-world cohort. PG - 118144 LID - S8756-3282(26)00370-4 [pii] LID - 10.1016/j.bone.2026.118144 [doi] AB - BACKGROUND: Automated laboratory detection of hypercalcemia may facilitate the recognition of previously unrecognized primary hyperparathyroidism (PHPT), but its value in routine clinical practice remains poorly defined, particularly with regard to subsequent etiological evaluation, healthcare resource utilization, and biochemical follow-up. METHODS: This retrospective cohort study evaluated an automated laboratory case-finding strategy for hypercalcemia across a healthcare area serving more than 230,000 inhabitants. Consecutive laboratory alerts generated between 2012 and 2020 were reviewed. After duplicate alerts were removed, 685 unique patients constituted the laboratory-detected cohort, of whom 258 underwent sufficient etiological evaluation and constituted the fully evaluated cohort. Clinical, biochemical, imaging, therapeutic, and longitudinal follow-up data were collected. RESULTS: Among the 258 fully evaluated patients, definite PHPT was diagnosed in 229 (88.8%). Eight additional patients were retrospectively considered to have probable mild PHPT with concomitant vitamin D deficiency, while other etiologies included tertiary hyperparathyroidism associated with chronic kidney disease, malignancy-associated hypercalcemia, medication-related hypercalcemia, familial hypocalciuric hypercalcemia, sarcoidosis, and vitamin D intoxication. Patients underwent a median of seven specialist consultations. Among patients with an available date of definitive etiological diagnosis, the median interval from the initial laboratory alert to diagnosis was 432 days (IQR 94.5-1219.5). During follow-up, 219/258 patients (84.9%) had at least one documented normocalcemic measurement after biochemical confirmation of persistent hypercalcemia, and 150/258 (58.1%) fulfilled the definition of sustained normocalcemia. In Kaplan-Meier analysis, the median time to first documented normocalcemia was 595 days (95% CI 505-780). CONCLUSIONS: Automated laboratory case-finding can identify patients with previously unrecognized persistent hypercalcemia and provide an entry point for subsequent etiological evaluation. PHPT was the predominant diagnosis within the fully evaluated cohort, although post-detection selection must be considered when interpreting this proportion. The prolonged diagnostic pathway and substantial healthcare use observed after the initial laboratory alert highlight the need to link automated detection strategies to structured clinical follow-up. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Roig-Marin, Noel AU - Roig-Marin N AD - Facultad de Medicina, Universidad Miguel Hernandez, Campus de San Juan de Alicante, Alicante, Spain. Electronic address: noel.roig@goumh.umh.es. FAU - Lopez-Garrigos, Maite AU - Lopez-Garrigos M AD - Clinical Laboratory Department, Hospital Universitario de San Juan, San Juan de Alicante, Spain; Department of Biochemistry, Universidad Miguel Hernandez, 03202, Elche, Spain. FAU - Flores, Emilio AU - Flores E AD - Clinical Laboratory Department, Hospital Universitario de San Juan, San Juan de Alicante, Spain; Department of Clinical Medicine, Universidad Miguel Hernandez, 03550, Sant Joan d'Alacant, Spain. FAU - Segui-Ripoll, Jose Miguel AU - Segui-Ripoll JM AD - Department of Clinical Medicine, Universidad Miguel Hernandez, 03550, Sant Joan d'Alacant, Spain; Department of Internal Medicine, University Hospital of San Juan, Alicante, Spain. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Bone JT - Bone JID - 8504048 SB - IM OTO - NOTNLM OT - Automated laboratory case-finding OT - Diagnostic pathway OT - Hypercalcemia OT - Laboratory alerts OT - Primary hyperparathyroidism OT - Real-world evidence COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/07/15 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S8756-3282(26)00370-4 [pii] AID - 10.1016/j.bone.2026.118144 [doi] PST - aheadofprint SO - Bone. 2026 Oct 28:118144. doi: 10.1016/j.bone.2026.118144. PMID- 42951158 OWN - NLM STAT- Publisher LR - 20261028 IS - 1096-0279 (Electronic) IS - 1046-5928 (Linking) DP - 2026 Oct 28 TI - Fabrication of a monoclonal antibody-based electro-immunosensor to detect SARS-CoV-2 nucleocapsid antigen. PG - 107030 LID - S1046-5928(26)00153-1 [pii] LID - 10.1016/j.pep.2026.107030 [doi] AB - The nucleocapsid protein is one of the major structural proteins of the SARS-CoV-2 virus, which has caused the COVID-19 pandemic. Due to their high abundance in the host circulation system, post-early infection, and their participation in other cyclic events that support viral survival, they exert control over the viral life cycle within the host. Thus, assessing these protein levels in serum can serve as an early diagnostic tool for COVID-19 infections. In the current study, monoclonal antibodies (mAbs) targeting recombinantly produced NCoV2 (rNCoV2) antigen have been developed, characterized, and assessed for their immunodiagnostic potential by indirect ELISA, sandwich ELISA, and further integrated into electro-immunosensor platforms. Hybridomas secreting anti-rNCoV2 mAbs were obtained by fusion of mouse myeloma Sp2/0 cells with rNCoV2 antigen-immunized murine splenocytes using a chemical fusion agent. Hybridoma clones PAb8E8c3-G8 of isotype IgG2A and PBa1-A2 of isotype IgG1, both bearing k light chains, were further developed and assessed for their diagnostic potential, as they have shown a stable growth pattern and the desired antibody response against the rNCoV2 antigen. The lowest concentration of antigen detected by both mAbs with confidence was 7.81 ng/well. Of the two, mAb PAb8E8c3-G8 was selected for mAb-based electro-immunosensor fabrication. A simple label-free GCE-carbon black-based electro-immunosensor was fabricated and enhanced with L-Cys/AuNP, onto which the mAb was immobilized. The integrated mAb electro-immunosensor could efficiently detect the rNCoV2 antigen, with LOD and LOQ values of 4.618 pg/mL and 15.24 pg/mL, respectively. Thus, in this study, we have generated mAbs against the rNCoV2 antigen, which have shown better diagnostic potential in terms of specificity and sensitivity. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Thangaraj, Prashanthi K AU - Thangaraj PK AD - Centre for Bioseparation Technology (CBST), Vellore Institute of Technology (VIT), Vellore, Tamil Nadu-632014, India. Electronic address: prashanthi.kt2020@vitstudent.ac.in. FAU - Jayaprakash, N S AU - Jayaprakash NS AD - Centre for Bioseparation Technology (CBST), Vellore Institute of Technology (VIT), Vellore, Tamil Nadu-632014, India. Electronic address: nsjayaprakash@vit.ac.in. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Protein Expr Purif JT - Protein expression and purification JID - 9101496 SB - IM OTO - NOTNLM OT - Nucleocapsid protein OT - SARS-CoV-2 virus OT - affinity purification OT - carbon black OT - electro-immunosensor OT - monoclonal antibody COIS- Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:36 PHST- 2026/07/16 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:36 [entrez] AID - S1046-5928(26)00153-1 [pii] AID - 10.1016/j.pep.2026.107030 [doi] PST - aheadofprint SO - Protein Expr Purif. 2026 Oct 28:107030. doi: 10.1016/j.pep.2026.107030. PMID- 42951156 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-2976 (Electronic) IS - 0960-8524 (Linking) DP - 2026 Oct 28 TI - Multi-omics insights into rare earth element recovery by microalgae under salinity gradients. PG - 136153 LID - S0960-8524(26)02235-2 [pii] LID - 10.1016/j.biortech.2026.136153 [doi] AB - Conventional rare earth element (REE) extraction methods are resource-intensive and generate secondary pollutants, whereas biomining using microorganisms such as microalgae offers a more sustainable alternative. Microalgal growth and metal recovery are strongly influenced by environmental factors, especially salinity; however, the molecular mechanisms driving REE recovery in microalgae exposed to saline conditions remain largely unexplored. The overarching aim of this study is to elucidate the effects of salinity on REE recovery by microalgae using a multi-omics framework. Specifically, nine microalgal strains were screened across salinities of 0-5 %, followed by integrated metabolomic, transcriptomic, and proteomic characterization of Limnospira maxima under control, REE-only, 3.5 % salinity, and combined REE-salinity conditions. Among the tested species, L. maxima exhibited the highest REE recovery efficiency, outperforming other microalgae by a factor of 2-10. In L. maxima, heavy REE (Sc, Y, Tb, and Dy) were recovered 10-15 % more efficiently than light REE (La, Pr, Nd), particularly under salinities between 0.5 % and 5 %. The selective recovery of REE by L. maxima was most pronounced at elevated salinities. Global metabolomic profiles of the combined treatment were primarily associated with salinity, suggesting that salinity establishes the dominant physiological state within which REE-associated responses occur. Multi-omics analysis showed distinct responses to REE and salinity stress, including changes in carbon fixation, photosynthesis, and amino acid and lipid metabolism, with both transcript-protein convergence and pathway-level discordance indicating complex cross-layer regulation. Overall, the results identify L. maxima as a promising candidate for REE recovery under saline conditions while defining molecular responses and knowledge gaps relevant to future development of microalgae-based REE separation. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Vo, Phong H N AU - Vo PHN AD - Climate Change Cluster, Faculty of Science, University of Technology Sydney, 15 Broadway, Ultimo, NSW 2007, Australia; School of Natural Sciences, Faculty of Science and Engineering, Macquarie University, NSW 2109, Australia. Electronic address: phong.vo@uts.edu.au. FAU - Songsomboon, Kittikun AU - Songsomboon K AD - Climate Change Cluster, Faculty of Science, University of Technology Sydney, 15 Broadway, Ultimo, NSW 2007, Australia. FAU - Kim, Mikael AU - Kim M AD - Climate Change Cluster, Faculty of Science, University of Technology Sydney, 15 Broadway, Ultimo, NSW 2007, Australia. FAU - Strezov, Vladimir AU - Strezov V AD - School of Natural Sciences, Faculty of Science and Engineering, Macquarie University, NSW 2109, Australia. FAU - Clark, Simon AU - Clark S AD - School of Engineering, Faculty of Science and Engineering, Macquarie University, NSW 2109, Australia. FAU - Kuzhiumparambil, Unnikrishnan AU - Kuzhiumparambil U AD - Climate Change Cluster, Faculty of Science, University of Technology Sydney, 15 Broadway, Ultimo, NSW 2007, Australia. FAU - Pernice, Mathieu AU - Pernice M AD - Climate Change Cluster, Faculty of Science, University of Technology Sydney, 15 Broadway, Ultimo, NSW 2007, Australia. FAU - Ralph, Peter J AU - Ralph PJ AD - Climate Change Cluster, Faculty of Science, University of Technology Sydney, 15 Broadway, Ultimo, NSW 2007, Australia. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Bioresour Technol JT - Bioresource technology JID - 9889523 SB - IM OTO - NOTNLM OT - Metabolomics OT - Microalgae OT - Proteomics OT - Rare earth elements OT - Salinity OT - Transcriptomics COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:35 PHST- 2026/07/31 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:35 [entrez] AID - S0960-8524(26)02235-2 [pii] AID - 10.1016/j.biortech.2026.136153 [doi] PST - aheadofprint SO - Bioresour Technol. 2026 Oct 28:136153. doi: 10.1016/j.biortech.2026.136153. PMID- 42951154 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-2976 (Electronic) IS - 0960-8524 (Linking) DP - 2026 Oct 28 TI - Comparison of two genetic systems for protein production in Cupriavidus necator H16. PG - 136154 LID - S0960-8524(26)02236-4 [pii] LID - 10.1016/j.biortech.2026.136154 [doi] AB - Many chemical manufacturing routes are being replaced with enzymatic processes to improve sustainability. In biocatalysis, enzyme production is often the main bottleneck. Bacterial proteins are frequently produced using the workhorse E. coli BL21(DE3) and its derivatives. However, other bacteria with beneficial characteristics can also be engineered for this purpose. Cupriavidus necator H16 (C. necator), for example, is a Gram-negative bacterium well-known for its chemolithoautotrophic metabolism and high polyhydroxybutyrate (PHB) accumulation. Previous studies have demonstrated protein production without inclusion body formation, which is one of the main challenges when producing enzymes in E. coli. Nevertheless, high-yield protein production in C. necator remains an understudied field. Here, we investigated the bottlenecks limiting protein production in C. necator. We optimized a T7 RNA polymerase genetic system and quantified the impact of several genetic elements such as T7 promoter, RBS strength and codon usage towards GFP production. Codon usage was the main factor limiting protein production in C. necator. Tuning the RBS strength and selecting a different T7 promoter strongly influenced expression leakiness. We then produced the ene-reductase YqjM from Bacillus subtilis in C. necator, analyzing the performance of the engineered C. necator strain and our previously developed pMVRha expression plasmid. C. necator produced high amounts of soluble protein and one of the strains produced higher amounts of FMN-loaded enzymes compared to E. coli BL21(DE3) in the conditions tested. This result highlights the potential of non-model bacteria to achieve high-yield enzyme production and promote the transition to biocatalysis-driven chemical synthesis. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Vajente, Matteo AU - Vajente M AD - Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Groningen 9713AV, the Netherlands. FAU - Hallamaa, Marleen AU - Hallamaa M AD - Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Groningen 9713AV, the Netherlands. FAU - Ballerstedt, Hendrik AU - Ballerstedt H AD - Institute of Applied Microbiology (iAMB), Aachen Biology and Biotechnology (ABBt), RWTH Aachen University, WSS research centre "catalaix", Worringerweg 1, 52074 Aachen, Germany. FAU - Blank, Lars Mathias AU - Blank LM AD - Institute of Applied Microbiology (iAMB), Aachen Biology and Biotechnology (ABBt), RWTH Aachen University, WSS research centre "catalaix", Worringerweg 1, 52074 Aachen, Germany. FAU - Schmidt, Sandy AU - Schmidt S AD - Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Groningen 9713AV, the Netherlands. Electronic address: s.schmidt@rug.nl. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Bioresour Technol JT - Bioresource technology JID - 9889523 SB - IM OTO - NOTNLM OT - Biocatalysis OT - Enzyme production OT - FMN cofactor OT - Metabolic engineering OT - T7 RNA polymerase COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:35 PHST- 2026/07/11 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:35 [entrez] AID - S0960-8524(26)02236-4 [pii] AID - 10.1016/j.biortech.2026.136154 [doi] PST - aheadofprint SO - Bioresour Technol. 2026 Oct 28:136154. doi: 10.1016/j.biortech.2026.136154. PMID- 42951144 OWN - NLM STAT- Publisher LR - 20261028 IS - 1083-351X (Electronic) IS - 0021-9258 (Linking) DP - 2026 Oct 28 TI - Dysfunction of three CD36 variants is due to impaired glycosylation and trafficking. PG - 113705 LID - S0021-9258(26)02577-9 [pii] LID - 10.1016/j.jbc.2026.113705 [doi] AB - CD36 deficiency is characterized by the loss of CD36 due to genetic mutations. Clinical studies have associated CD36 deficiency with hyperlipidemia, insulin resistance, and cardiomyopathy. However, the structural and functional effects of human CD36 variants remain poorly understood. P90S is a causative mutation of CD36 deficiency while E123K and S127L are of unknown significance and typically found in malarial endemic regions. Despite their variable phenotypes, they are all located in the same structural region of CD36. In this study, we expressed these three naturally occurring human CD36 variants in HEK293 cells and found that all variants exhibited impaired oxLDL binding and uptake that was associated with reduced expression, shortened half-lives, and altered glycan processing, consistent with defects in receptor maturation. Inhibition of proteasomal or lysosomal pathways did not restore variant stability, suggesting that reduced receptor abundance is not mediated by a single canonical degradation pathway. All variants exhibited reduced membrane accumulation without enrichment in specific intracellular compartments, indicating impaired trafficking rather than selective retention. These findings provide mechanistic insight into the regulation of human CD36 expression, maturation, and trafficking, and the functional consequences of genetic variation. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Tillison, Emma A AU - Tillison EA AD - Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA. FAU - Jovanovic, Vuk AU - Jovanovic V AD - Department of Medicine, Medical College of Wisconsin, Milwaukee, WI, USA. FAU - Luu, Quoc Quang AU - Luu QQ AD - Versiti Blood Research Institute, Milwaukee, WI, USA. FAU - Chen, Yiliang AU - Chen Y AD - Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA; Department of Medicine, Medical College of Wisconsin, Milwaukee, WI, USA; Versiti Blood Research Institute, Milwaukee, WI, USA; Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, WI, USA. FAU - Sahoo, Daisy AU - Sahoo D AD - Department of Biochemistry, Medical College of Wisconsin, Milwaukee, WI, USA; Department of Medicine, Medical College of Wisconsin, Milwaukee, WI, USA; Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, WI, USA. Electronic address: dsahoo@mcw.edu. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Biol Chem JT - The Journal of biological chemistry JID - 2985121R SB - IM OTO - NOTNLM OT - CD36 OT - Scavenger receptor OT - confocal microscopy OT - glycosylation OT - human variants OT - oxidized LDL OT - transmembrane proteins EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:35 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:35 [entrez] AID - S0021-9258(26)02577-9 [pii] AID - 10.1016/j.jbc.2026.113705 [doi] PST - aheadofprint SO - J Biol Chem. 2026 Oct 28:113705. doi: 10.1016/j.jbc.2026.113705. PMID- 42951139 OWN - NLM STAT- Publisher LR - 20261028 IS - 1083-351X (Electronic) IS - 0021-9258 (Linking) DP - 2026 Oct 28 TI - THE UBC8-GID E3 UBIQUITIN LIGASE MODULE IS REQUIRED FOR STRESS RESPONSE IN CANDIDA ALBICANS. PG - 113701 LID - S0021-9258(26)02573-1 [pii] LID - 10.1016/j.jbc.2026.113701 [doi] AB - Candida albicans is a leading cause of fungal infections in humans. To survive and proliferate within diverse and dynamic host niches, Candida must rapidly adapt, efficiently acquire essential nutrients, and mount protective stress responses. While cellular stress signalling networks are well described, regulation via the ubiquitin system, including E2-E3 ubiquitylation modules, remains largely unexplored in Candida. Here we investigate the multiprotein Glucose Induced Degradation Deficient (GID) E3 ubiquitin ligase complex and its cognate E2 conjugating enzyme, CaUbc8. Utilizing mass spectrometry-based proteomics, we identified conserved GID subunits, including the previously unannotated CaGid5 homologue. AlphaFold3 modelling predicted a conserved CaUbc8-GID E3 complex architecture, with modular features similar to the homologous Saccharomyces cerevisiae GID E3 complex, with CaGid5 functioning as the adaptor for the interchangeable substrate receptors CaGid4 and CaGid11. We observed that Candida cells responded to different stresses, including carbon source switching, heat shock, and osmotic stress, via a dynamic and differential expression of CaGid4 and CaGid11, suggesting the conditional assembly of distinct GID(CaGid4) and GID(CaGid11) E3 complexes. Notably, copper stress selectively enhanced the expression of CaGid4. Absence of CaGid4 receptor, GID E3 catalytic activity, or Ubc8 reduced copper stress-induced Candida growth and viability, and attenuated antifungal drug efficacy. System-wide proteomics analysis revealed CaGid4-dependent proteome and interactome dynamics implying modulation of oxidative stress response pathways. Together, our studies identified the multi-stress-responsive CaUbc8-GID E3 complex required for antifungal drug efficacy in Candida albicans, and we propose it as a new tractable target for the development of candidiasis therapeutics. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Zeng, Zirong AU - Zeng Z AD - Centre for Inflammation Research, Institute for Regeneration and Repair, University of Edinburgh, UK; Edinburgh Medical School, College of Medicine and Veterinary Medicine, University of Edinburgh, UK. FAU - Chrustowicz, Jakub AU - Chrustowicz J AD - Department of Molecular Machines and Signaling, Max Planck Institute of Biochemistry, Martinsried, Germany. FAU - Spanos, Christos AU - Spanos C AD - Discovery Research Platform for Hidden Cell Biology, Centre for Cell Biology, University of Edinburgh, UK. FAU - Cholewa-Waclaw, Justyna AU - Cholewa-Waclaw J AD - High Content Screening Facility, Institute for Regeneration and Repair, University of Edinburgh, UK. FAU - Kelly, Van AU - Kelly V AD - School of Chemistry, University of Edinburgh, UK. FAU - Zhao, Yixin AU - Zhao Y AD - Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK. FAU - Scharf, Daniel H AU - Scharf DH AD - Department of Microbiology, School of Basic Medical Sciences, Zhejiang University School of Medicine, Hangzhou 310058, China. FAU - Munro, Carol A AU - Munro CA AD - Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK. FAU - Schulman, Brenda A AU - Schulman BA AD - Department of Molecular Machines and Signaling, Max Planck Institute of Biochemistry, Martinsried, Germany. FAU - Alpi, Arno F AU - Alpi AF AD - Institute for Cell Biology, University of Edinburgh, UK. FAU - Makrantoni, Vasso AU - Makrantoni V AD - Centre for Inflammation Research, Institute for Regeneration and Repair, University of Edinburgh, UK; Edinburgh Medical School, College of Medicine and Veterinary Medicine, University of Edinburgh, UK; Centre for Infection Immunity and Cancer, Zhejiang University -University of Edinburgh (ZJU-UoE) Institute, Haining 314400, China. Electronic address: vasso.makrantoni@ed.ac.uk. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Biol Chem JT - The Journal of biological chemistry JID - 2985121R SB - IM OTO - NOTNLM OT - Candida albicans OT - E3 ubiquitin ligase OT - GID E3 OT - antifungal resistance OT - copper OT - stress response OT - ubiquitylation EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:35 PHST- 2026/03/19 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:35 [entrez] AID - S0021-9258(26)02573-1 [pii] AID - 10.1016/j.jbc.2026.113701 [doi] PST - aheadofprint SO - J Biol Chem. 2026 Oct 28:113701. doi: 10.1016/j.jbc.2026.113701. PMID- 42951138 OWN - NLM STAT- Publisher LR - 20261028 IS - 1083-351X (Electronic) IS - 0021-9258 (Linking) DP - 2026 Oct 28 TI - A case for DNA-membrane interactions in the responses to DNA damage. PG - 113708 LID - S0021-9258(26)02580-9 [pii] LID - 10.1016/j.jbc.2026.113708 [doi] AB - Eukaryotic cells harbour two genomes, the nuclear and the mitochondrial ones. Both are subjected to spontaneous and induced DNA damage that threatens biological function. In the nucleus, damage in the DNA is detected, signalled and taken care of by a dedicated, complex network of proteins termed the DNA Damage Response (DDR). In the mitochondria, this first-front DNA damage detection network is less characterized, despite that effective, downstream mitochondrial DNA (mtDNA) repair is documented. Also, given that mtDNA exists as multiple copies inside a single mitochondrion, the cell can selectively get rid of unhealthy mtDNA molecules. This later process suggests the existence of an additional quality control mechanism operating within mitochondria. Careful analysis of the literature suggests that damaged DNA can cause alterations in surrounding membrane structure / composition that could be integrated as an output to appropriately respond to such DNA damage. In this review, I discuss the evidence for membranes' involvement in the response to mtDNA damage. Finally, I discuss similarities and differences between mitochondrial and nuclear membranes and further extend the hypothesis to the possibility that membranes may play roles in signalling DNA damage that arise in the nuclear genome. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Moriel-Carretero, Maria AU - Moriel-Carretero M AD - Centre de Recherche en Biologie cellulaire de Montpellier (CRBM), Universite de Montpellier - Centre National de la Recherche Scientifique, France. Electronic address: maria.moriel@crbm.cnrs.fr. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - J Biol Chem JT - The Journal of biological chemistry JID - 2985121R SB - IM OTO - NOTNLM OT - DNA damage OT - DNA damage response OT - membrane OT - mitochondria OT - nuclear envelope EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:35 PHST- 2025/12/21 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:35 [entrez] AID - S0021-9258(26)02580-9 [pii] AID - 10.1016/j.jbc.2026.113708 [doi] PST - aheadofprint SO - J Biol Chem. 2026 Oct 28:113708. doi: 10.1016/j.jbc.2026.113708. PMID- 42951122 OWN - NLM STAT- Publisher LR - 20261028 IS - 1878-5476 (Electronic) IS - 1878-5476 (Linking) DP - 2026 Oct 28 TI - Sexual Violence in Sport and a Dual Harm Hypothesis of Victimization and Disrupted Belonging: A Systematic Review. PG - 103278 LID - S1469-0292(26)00219-0 [pii] LID - 10.1016/j.psychsport.2026.103278 [doi] AB - Sexual violence (SV) in sport is a public health and safeguarding concern, but evidence across prevalence, abuse dynamics, risk factors, and consequences remains fragmented. This systematic review synthesized quantitative, qualitative, and mixed-methods research across these four domains. Eight databases were searched from inception to July 2025. Study selection was conducted independently by three reviewers, methodological quality was appraised using the Mixed Methods Appraisal Tool, and findings were synthesized narratively. Sixty-three publications representing 58 independent studies were included. Study-level prevalence estimates ranged from below 2% to 61% and reached 70% in a female subgroup. Much of this dispersion reflected differences in construct breadth, setting, perpetrator scope, and recall period. Estimates were more similar within matched strata. Abuse dynamics involved grooming, progressive boundary violations, career dependency, and perpetrators extending beyond coaches to peers, medical personnel, and multi-offender configurations. Risk factors operated across individual, interpersonal, organizational, and sociocultural levels, with relational power, organizational structures, and cultures of silence emerging as important enabling conditions. Consequences included adverse mental, social, and sexual health outcomes, non-disclosure, institutional betrayal, relational withdrawal, and disengagement from sport. These findings provide a basis for hypothesizing that SV in sport may produce a sport-specific dual harm, combining the multidimensional injury of victimization with disrupted belonging and participation in sport. Hence, effective safeguarding requires multilevel, evidence-informed prevention that addresses structural and cultural conditions alongside individual behavior. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Carmo, Eunice AU - Carmo E AD - Center for Psychology at University of Porto, Faculty of Psychology and Educational Sciences, University of Porto, Rua Alfredo Allen, 4200-135 Porto, Portugal; William James Center for Research, Department of Education and Psychology, University of Aveiro, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal. Electronic address: Up201810040@edu.med.up.pt. FAU - Moreira, Barbara AU - Moreira B AD - Center for Psychology at University of Porto, Faculty of Psychology and Educational Sciences, University of Porto, Rua Alfredo Allen, 4200-135 Porto, Portugal. FAU - Azevedo, Vanessa AU - Azevedo V AD - Center for Psychology at University of Porto, Faculty of Psychology and Educational Sciences, University of Porto, Rua Alfredo Allen, 4200-135 Porto, Portugal; Department of Social and Behavioural Sciences, University of Maia, Maia, Portugal. FAU - Carvalho, Joana AU - Carvalho J AD - William James Center for Research, Department of Education and Psychology, University of Aveiro, Campus Universitario de Santiago, 3810-193 Aveiro, Portugal. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - Netherlands TA - Psychol Sport Exerc JT - Psychology of sport and exercise JID - 101088724 SB - IM OTO - NOTNLM OT - Athletes OT - Public Health OT - Safeguarding OT - Sexual Violence in Sport OT - Systematic Review COIS- Declaration of Competing Interest The authors received no financial support for the research, authorship, or publication of this article and declare no conflicts of interest. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:35 PHST- 2026/05/07 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:35 [entrez] AID - S1469-0292(26)00219-0 [pii] AID - 10.1016/j.psychsport.2026.103278 [doi] PST - aheadofprint SO - Psychol Sport Exerc. 2026 Oct 28:103278. doi: 10.1016/j.psychsport.2026.103278. PMID- 42951121 OWN - NLM STAT- Publisher LR - 20261028 IS - 1096-1186 (Electronic) IS - 1043-6618 (Linking) DP - 2026 Oct 28 TI - Shugan Xiaozhi Formula Reduces Hepatic Steatosis and Stiffness in MASLD Patients with Elevated ALT: An Exploratory 24-Week Randomized Trial. PG - 109705 LID - S1043-6618(26)01620-8 [pii] LID - 10.1016/j.phrs.2026.109705 [doi] AB - BACKGROUND: Metabolic dysfunction-related fatty liver disease (MASLD) affects approximately one-third of adults worldwide. The most common manifestations in the early stage are liver dysfunction, hepatic steatosis，metabolic abnormalities, and obesity. The failure of single behavioral interventions and the limited availability of treatment drugs remain unmet clinical needs. METHODS: In this 24-week, multicenter, prospective, randomized, open-label, active-controlled, proof-of-concept trial conducted in China, 285 participants with MASLD (ALT >40U/L to 5xULN) were randomly assigned in a 1:1 ratio to receive Shugan Xiaozhi Formula (SGXZ) granules or vitamin E 300mg/day. The primary exploratory endpoints were changes in hepatic steatosis measured by MRI-proton density fat fraction (MRI-PDFF) and FibroScan-controlled attenuation parameter (CAP), and changes in liver stiffness by transient elastography (TE) and magnetic resonance elastography (MRE). Additional outcomes included liver enzymes, anthropometric measures, and safety parameters. Analyses were performed in the completer population (n=152) using ANCOVA and logistic regression. RESULTS: At week 24, SGXZ significantly reduced MRI-PDFF (adjusted mean difference vs vitamin E, -3.44 percentage points; 95% CI, -5.18 to -1.69; P<0.001) and CAP (-45.42dB/m; 95% CI, -58.37 to -32.47; P<0.001). A higher proportion of SGXZ recipients achieved a >/=1-category reduction in MRI-PDFF-defined steatosis grade (22.4% vs 5.3%; OR, 5.83; P=0.007) and a >/=30dB/m reduction in CAP (82.9% vs 39.5%; OR, 8.01; P<0.001). Liver stiffness parameters also improved more with SGXZ (TE, -1.14 kPa; P<0.001; MRE, -0.06m/s; P<0.001). Changes in ALT and AST did not differ between groups, whereas GGT normalization was more frequent with SGXZ (52.3% vs 27.7%; OR, 2.87; P=0.010). SGXZ was also associated with significantly greater reductions in body weight (between-group mean difference, -2.69kg; 95% CI, -3.60 to -1.78; P < 0.001), BMI (-0.94kg/m(2); 95% CI, -1.26 to -0.62; P < 0.001), and WHR (-0.04; 95% CI, -0.08 to -0.01; P = 0.020). SGXZ was generally well tolerated; gastrointestinal events were more common but were mild and self-limiting. No serious adverse events or clinically meaningful laboratory safety signals occurred. CONCLUSIONS: In this exploratory trial, SGXZ was associated with greater reductions in hepatic steatosis, imaging-based liver stiffness, body weight, BMI, and WHR than vitamin E, with an acceptable safety profile. These findings suggest that SGXZ represents a highly promising compound natural medicine therapeutic option for the complex metabolic diseases MASLD/MASH. It meets the urgent clinical need for safe and effective therapies to comprehensively improve patients' conditions. Of course, these findings require further validation in large-scale trials with sufficient statistical power. CLINICAL TRIAL REGISTRATION NUMBER: ChiCTR2200057595. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Sun, Yuxin AU - Sun Y AD - Department of Hepatology, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen 518033, China. FAU - Han, Zhiyi AU - Han Z AD - Department of Hepatology, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen 518033, China. FAU - Li, Can AU - Li C AD - The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, PR China; Liuzhou Traditional Chinese Medicine Hospital (Liuzhou Zhuang Medicine Hospital), Liuzhou, Guangxi Zhuang Autonomous Region, PR China. FAU - Hu, Die AU - Hu D AD - Shenzhen Traditional Chinese Medicine Hospital Affiliated to Nanjing University of Chinese Medicine, Shenzhen, PR China. FAU - Xie, Fanci AU - Xie F AD - The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, PR China; Department of General Medicine, Longhua District People's Hospital, Shenzhen, PR China. FAU - Li, Qingqing AU - Li Q AD - The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, PR China. FAU - Luo, Hong AU - Luo H AD - The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, PR China. FAU - Liao, Mianmian AU - Liao M AD - Dongguan Key Laboratory of Traditional Chinese Medicine Hepatology, the Affiliated Dongguan Songshan Lake Central Hospital, Guangdong Medical University, Dongguan 523326, China. FAU - Zhou, Xiaoling AU - Zhou X AD - Liuzhou Traditional Chinese Medicine Hospital (Liuzhou Zhuang Medicine Hospital), Liuzhou, Guangxi Zhuang Autonomous Region, PR China. FAU - Hu, Jingbao AU - Hu J AD - The Seventh Clinical College of Guangzhou University of Chinese Medicine, Shenzhen, PR China. FAU - Mo, Shizao AU - Mo S AD - Tengxian Hospital of Traditional Chinese Medicine, Guangxi Zhuang Autonomous Region, PR China. FAU - Cai, Nailiang AU - Cai N AD - Sanya People's Hospital, Jiefang Road, Tianya District, Sanya, Hainan, PR China. FAU - Ma, Xiaojun AU - Ma X AD - Foshan Hospital of Traditional Chinese Medicine, No. 6 Qinren Road, Foshan, PR China. FAU - Lai, Yu AU - Lai Y AD - Meizhou Hospital of Traditional Chinese Medicine, Meizhou, Guangdong, PR China. FAU - Zhang, Shijun AU - Zhang S AD - The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan Er Road, Guangzhou, PR China. FAU - Li, Dan AU - Li D AD - China Resources Sanjiu Modern Chinese Medicine Pharmaceutical Co., Ltd., PR China. FAU - Xing, Yufeng AU - Xing Y AD - Department of Hepatology, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen 518033, China. Electronic address: yufeng000729@163.com. FAU - Zhou, Hua AU - Zhou H AD - Chinese Medicine Guangdong Laboratory (Hengqin Laboratory), State Key Laboratory of Traditional Chinese Medicine Syndrome, State Key Laboratory of Dampness Syndrome of Chinese Medicine, Guangdong Provincial Key Laboratory of Chinese Medicine for Prevention and Treatment of Refractory Chronic Diseases, Guangdong Provincial Hospital of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangdong, PR China. Electronic address: gutcmzhs@hotmail.com. FAU - Tong, Guangdong AU - Tong G AD - Department of Hepatology, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen 518033, China. Electronic address: tgd755@163.com. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Pharmacol Res JT - Pharmacological research JID - 8907422 SB - IM OTO - NOTNLM OT - MASLD OT - MRI-PDFF OT - Shugan Xiaozhi Formula OT - herbal medicine OT - non-invasive imaging OT - randomized trial COIS- Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Guangdong Tong reports equipment, drugs, or supplies was provided by the China Resources Sanjiu Modern Chinese Medicines Pharmaceutical Co., Ltd. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:35 PHST- 2026/08/24 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:35 [entrez] AID - S1043-6618(26)01620-8 [pii] AID - 10.1016/j.phrs.2026.109705 [doi] PST - aheadofprint SO - Pharmacol Res. 2026 Oct 28:109705. doi: 10.1016/j.phrs.2026.109705. PMID- 42951120 OWN - NLM STAT- Publisher LR - 20261028 IS - 1096-1186 (Electronic) IS - 1043-6618 (Linking) DP - 2026 Oct 28 TI - Targeting the Catalytic and ZnF-UBP Domains of HDAC6 Differentially Controls NLRP3 Inflammasome Priming and Assembly in Paclitaxel-Induced Peripheral Neuropathy. PG - 109711 LID - S1043-6618(26)01626-9 [pii] LID - 10.1016/j.phrs.2026.109711 [doi] AB - Paclitaxel-induced peripheral neuropathy (PIPN) is a dose-limiting complication of cancer chemotherapy driven by macrophage-mediated NLRP3 inflammasome activation. While suppressing TLR-NF-kappaB-dependent priming can limit neuroinflammation by reducing both NLRP3 induction and downstream inflammasome activation, such upstream intervention may also affect broader immune transcriptional programs in vulnerable oncology patients. Here, we identify a structural division of labor within histone deacetylase 6 (HDAC6) that pharmacologically uncouples the priming and assembly steps of NLRP3 activation. In a murine PIPN model, we demonstrate that paclitaxel directly primes macrophages. Complementary ND7/23-BMDM experiments support a model in which ATP released from paclitaxel-stressed neuronal cells provides a second signal for inflammasome assembly. By integrating domain-selective pharmacology with genetic knockdown-rescue using catalytically inactive and ZnF-UBP-deletion mutants, we reveal that the catalytic domain of HDAC6 governs upstream TLR4-NF-kappaB-dependent priming. In contrast, the non-catalytic ZnF-UBP domain is required for downstream HDAC6-NLRP3-ASC complexation and ASC speck assembly, ultimately driving caspase-1 activation, GSDMD cleavage, and IL-1beta release. In vivo, targeting the ZnF-UBP domain with CP31 effectively alleviated paclitaxel-evoked pain hypersensitivity and dorsal root ganglion (DRG) neuroinflammation without altering canonical alpha-tubulin acetylation. By reframing HDAC6 into domain-separable intervention points, we provide a pathway-restricted therapeutic strategy for PIPN that precisely disarms downstream pathological inflammasome assembly while largely preserving the measured upstream priming responses. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Ying, Haoran AU - Ying H AD - School of Pharmacy, Shenzhen University Medical School, Shenzhen University, Shenzhen 518055, China. FAU - Yue, Ruizhu AU - Yue R AD - School of Pharmacy, Shenzhen University Medical School, Shenzhen University, Shenzhen 518055, China. FAU - Zhang, Jin AU - Zhang J AD - School of Pharmacy, Shenzhen University Medical School, Shenzhen University, Shenzhen 518055, China. FAU - Guo, Xing AU - Guo X AD - School of Pharmacy, Shenzhen University Medical School, Shenzhen University, Shenzhen 518055, China. FAU - Li, Jinsong AU - Li J AD - School of Pharmacy, Shenzhen University Medical School, Shenzhen University, Shenzhen 518055, China. FAU - Mo, Wenyu AU - Mo W AD - School of Pharmacy, Shenzhen University Medical School, Shenzhen University, Shenzhen 518055, China. FAU - Hao, Yue AU - Hao Y AD - School of Pharmacy, Shenzhen University Medical School, Shenzhen University, Shenzhen 518055, China. Electronic address: yuehao@szu.edu.cn. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Pharmacol Res JT - Pharmacological research JID - 8907422 SB - IM OTO - NOTNLM OT - Domain-selective targeting OT - HDAC6 OT - NLRP3 inflammasome OT - Neuroinflammation OT - Paclitaxel-induced peripheral neuropathy OT - Zinc-finger ubiquitin-binding domain COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Competing interests The authors declare that they have no competing interests. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:35 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:35 [entrez] AID - S1043-6618(26)01626-9 [pii] AID - 10.1016/j.phrs.2026.109711 [doi] PST - aheadofprint SO - Pharmacol Res. 2026 Oct 28:109711. doi: 10.1016/j.phrs.2026.109711. PMID- 42951117 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-1131 (Electronic) IS - 0738-081X (Linking) DP - 2026 Oct 28 TI - Melanoma risk in astronauts: Clinical screening, surveillance, and the microgravity paradox. LID - S0738-081X(26)00307-X [pii] LID - 10.1016/j.clindermatol.2026.10.011 [doi] AB - Astronauts show significantly elevated melanoma incidence and mortality relative to the general population, a finding with direct relevance to an expanding civilian spaceflight population. We conducted a systematic review to characterize this risk and translate it into practical guidance for dermatologists. Of 11 studies meeting eligibility criteria, only one was a human clinical or epidemiological study; the remainder were experimental, and consistently showed that simulated microgravity suppresses melanoma proliferation, adhesion, migration, and invasion while promoting apoptosis through collapse of FAK-RhoA-mTORC1-ERK1/2 mechanotransduction, a laboratory finding in direct tension with the clinical risk observed in astronauts. The majority of astronauts studied were career pilots, an occupational group with an independently established approximately two-fold melanoma incidence and elevated mortality specifically among pilots from cockpit UVA exposure accrued before spaceflight, which we identify as the most clinically substantial explanation for this paradox. We recommend risk-stratified pre-flight total-body skin examination with dermoscopy, deferral of dermatologic management to the ground rather than in-flight, post-flight surveillance after long-duration missions, and individualized counseling that distinguishes career astronauts from the demographically distinct civilian spaceflight population now emerging through missions such as Inspiration4 and Polaris Dawn. CI - Copyright (c) 2026. Published by Elsevier Inc. CN - Space Dermatology Consortium LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Clin Dermatol JT - Clinics in dermatology JID - 8406412 SB - IM OTO - NOTNLM OT - Astronaut health OT - Melanoma OT - Microgravity OT - Skin cancer screening OT - Space medicine COIS- Declaration of competing interests None FIR - Akbarialiabad, Hossein IR - Akbarialiabad H IRAD- Oregon Health & Science University, Portland, OR, USA; School of Clinical Medicine, UNSW Medicine & Health, University of New South Wales, Sydney, NSW, Australia. FIR - Khayatan, Danial IR - Khayatan D IRAD- Department of Dermatology, Columbia University Irving Medical Center, New York, NY, USA. FIR - Taghrir, Mohammad Hossein IR - Taghrir MH IRAD- School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. FIR - Leachman, Sancy A IR - Leachman SA IRAD- Department of Dermatology, Oregon Health & Science University, Portland, OR, USA. FIR - Grant-Kels, Jane M IR - Grant-Kels JM IRAD- Department of Dermatology, University of Connecticut School of Medicine, Farmington, CT, USA; Department of Dermatology, University of Florida College of Medicine, Gainesville, FL, USA. FIR - Melin, Matthew Mark IR - Melin MM IRAD- Gonda Vascular Center, Wound Clinic, Mayo Clinic, Rochester, MN, USA. FIR - Beheshti, Afshin IR - Beheshti A IRAD- McGowan Institute for Regenerative Medicine, Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA; Center for Space Biomedicine, Trivedi Institute for Space and Global Biomedicine, University of Pittsburgh, Pittsburgh, PA, USA; Department of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA, USA; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA, USA. FIR - Costes, Sylvain V IR - Costes SV IRAD- Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA; Department of Radiation Oncology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. FIR - Bunick, Christopher G IR - Bunick CG IRAD- Department of Dermatology, Program in Translational Biomedicine, Institute for Global Health, Yale University School of Medicine, New Haven, CT, USA. FIR - Mostaghimi, Arash IR - Mostaghimi A IRAD- Department of Dermatology, Brigham and Women's Hospital, Boston, MA, USA. FIR - Christiansen, Rowena IR - Christiansen R IRAD- Department of Medical Education, Melbourne Medical School, The University of Melbourne, Melbourne, VIC, Australia. FIR - El-Assaad, Fatima IR - El-Assaad F IRAD- University of New South Wales Microbiome Research Centre, School of Clinical Medicine, UNSW Medicine & Health, St George & Sutherland Clinical Campuses, Sydney, NSW, Australia. FIR - Grada, Ayman IR - Grada A IRAD- Department of Dermatology, Case Western Reserve University School of Medicine, Cleveland, OH, USA. Electronic address: ayman.grada@case.edu. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:35 PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:35 [entrez] AID - S0738-081X(26)00307-X [pii] AID - 10.1016/j.clindermatol.2026.10.011 [doi] PST - aheadofprint SO - Clin Dermatol. 2026 Oct 28:S0738-081X(26)00307-X. doi: 10.1016/j.clindermatol.2026.10.011. PMID- 42951106 OWN - NLM STAT- Publisher LR - 20261028 IS - 1469-0691 (Electronic) IS - 1198-743X (Linking) DP - 2026 Oct 28 TI - Beyond microbiology: sociocultural, governance and economic determinants of foodborne outbreaks-lessons from the 2026 cyclosporiasis outbreak in the United States. LID - S1198-743X(26)00600-2 [pii] LID - 10.1016/j.cmi.2026.10.022 [doi] AB - BACKGROUND: Foodborne outbreaks reflect both biological hazards and the systems through which food is produced, distributed, consumed and regulated. The 2026 cyclosporiasis outbreak linked to processed iceberg lettuce in the United States shows how epidemiology, traceback, laboratory findings and regulatory action can evolve during the same investigation. OBJECTIVES: To examine how social, cultural, regulatory, institutional and economic factors influence the generation, recognition, source attribution, spread, control and consequences of foodborne outbreaks. SOURCES: We searched PubMed and Embase using reproducible, broad and event-specific strategies through 16 October 2026, supplemented by public health and regulatory websites. Titles and abstracts were screened to identify reports that could contribute a distinct mechanistic or contextual lesson; candidate events were then selected purposively. CONTENT: The 2026 outbreak was associated with 12,883 laboratory-confirmed illnesses, 570 hospitalisations and two deaths. Epidemiological and traceback analyses identified processed iceberg lettuce as the vehicle of the outbreak and a single supplier in central Mexico as the implicated source. A product sample was reclassified as a false positive; later on-site sampling detected Cyclospora in outgoing wastewater from the supplier's processing facility and in a drainage ditch at an implicated grower, but the precise route of contamination remained unresolved. Historical examples illustrate how changes in attribution, food insecurity, cultural practices, communication, commercial incentives, industrial concentration, and host vulnerability can shape outbreaks. IMPLICATIONS: These examples suggest practical considerations rather than formal recommendations. Outbreak reports are easier to interpret when epidemiological, traceback and laboratory evidence are described separately, and changes in attribution are explained promptly. Convergent epidemiology and traceback can support proportionate control even when product testing is negative or unavailable. The proposed framework is an interpretive synthesis, not a validated causal model. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved. FAU - Gupta, Nitin AU - Gupta N AD - Division of Tropical Medicine, Department of Infectious Diseases, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India; Emerging Infections Subcommittee (EIS), European Society of Clinical Microbiology and Infectious Diseases (ESCMID); Center of Tropical Medicine and Travel Medicine, Department of Infectious Diseases, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands. FAU - Grobusch, Martin P AU - Grobusch MP AD - Emerging Infections Subcommittee (EIS), European Society of Clinical Microbiology and Infectious Diseases (ESCMID); Center of Tropical Medicine and Travel Medicine, Department of Infectious Diseases, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands. Electronic address: m.p.grobusch@amsterdamumc.nl. FAU - Jokelainen, Pikka AU - Jokelainen P AD - Emerging Infections Subcommittee (EIS), European Society of Clinical Microbiology and Infectious Diseases (ESCMID); One Health and International Collaborations, Infectious Disease Preparedness, Statens Serum Institut, Copenhagen, Denmark. FAU - Tsiodras, Sotirios AU - Tsiodras S AD - Emerging Infections Subcommittee (EIS), European Society of Clinical Microbiology and Infectious Diseases (ESCMID); 4th Department of Internal Medicine and Infectious Diseases, Attikon University Hospital, National and Kapodistrian University of Athens Medical School, Athens, Greece. FAU - Barac, Aleksandra AU - Barac A AD - Emerging Infections Subcommittee (EIS), European Society of Clinical Microbiology and Infectious Diseases (ESCMID); Clinic for Infectious and Tropical Diseases, University Clinical Center of Serbia, Faculty of Medicine, University of Belgrade, Belgrade, Serbia. FAU - Mora-Rillo, Marta AU - Mora-Rillo M AD - Emerging Infections Subcommittee (EIS), European Society of Clinical Microbiology and Infectious Diseases (ESCMID); High Level Isolation Unit, La Paz University Hospital, IdiPAZ, CIBERINFEC, Madrid, Spain. FAU - Lescure, F-Xavier AU - Lescure FX AD - Emerging Infections Subcommittee (EIS), European Society of Clinical Microbiology and Infectious Diseases (ESCMID); Infectious and Tropical Diseases Department, APHP, Bichat Hospital and Universite Paris Cite, INSERM, IAME, Paris, France. FAU - Gkrania-Klotsas, Effrossyni AU - Gkrania-Klotsas E AD - Emerging Infections Subcommittee (EIS), European Society of Clinical Microbiology and Infectious Diseases (ESCMID); Department of Infectious Diseases, Cambridge University Hospitals NHS Trust and NIHR Cambridge Biomedical Research Centre, Cambridge, UK. FAU - Drexler, Jan Felix AU - Drexler JF AD - Emerging Infections Subcommittee (EIS), European Society of Clinical Microbiology and Infectious Diseases (ESCMID); Institute of Virology, Charite-Universitatsmedizin Berlin, corporate member of Freie Universitat Berlin and Humboldt-Universitat zu Berlin, Berlin, Germany. CN - Emerging Infections Subcommittee (EIS) of ESCMID LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - Clin Microbiol Infect JT - Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases JID - 9516420 SB - IM OTO - NOTNLM OT - One Health OT - cyclosporiasis OT - food systems OT - foodborne outbreaks OT - outbreak attribution COIS- Potential conflict of interest The authors declare no conflicts of interest relevant to this article. FIR - Barac, Aleksandra IR - Barac A IRAD- Clinic for Infectious and Tropical Diseases, University Clinical Center of Serbia, Faculty of Medicine, University of Belgrade, Belgrade, Serbia. Electronic address: aleksandrabarac85@gmail.com. FIR - Drexler, Jan Felix IR - Drexler JF IRAD- Institute of Virology, Charite-Universitatsmedizin Berlin, corporate member of Freie Universitat Berlin and Humboldt-Universitat zu Berlin, Berlin, Germany. Electronic address: felix.drexler@charite.de. FIR - Gkrania-Klotsas, Effrossyni IR - Gkrania-Klotsas E IRAD- Department of Infectious Diseases, Cambridge University Hospitals NHS Trust and NIHR Cambridge Biomedical Research Centre, Cambridge, UK. Electronic address: egkraniaklotsas@nhs.net. FIR - Grobusch, Martin P IR - Grobusch MP IRAD- Center of Tropical Medicine and Travel Medicine, Department of Infectious Diseases, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands. FIR - Gupta, Nitin IR - Gupta N IRAD- Department of Infectious Diseases, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Karnataka, India. Electronic address: nitin.gupta@manipal.edu. FIR - Jokelainen, Pikka IR - Jokelainen P IRAD- One Health and International Collaborations, Infectious Disease Preparedness, Statens Serum Institut, Copenhagen, Denmark. Electronic address: PIJO@ssi.dk. FIR - Lescure, F-Xavier IR - Lescure FX IRAD- Infectious and Tropical Diseases Department, APHP, Bichat Hospital and Universite Paris Cite, INSERM, IAME, Paris, France. Electronic address: xavier.lescure@aphp.fr. FIR - Mora-Rillo, Marta IR - Mora-Rillo M IRAD- High Level Isolation Unit, La Paz University Hospital, IdiPAZ, CIBERINFEC, Madrid, Spain. Electronic address: marta.mora@salud.madrid.org. FIR - Pano-Pardo, Jose-Ramon IR - Pano-Pardo JR IRAD- Division of Infectious Diseases, Hospital Clinico Universitario, Zaragoza, Spain. Electronic address: joserrapa@gmail.com. FIR - Pellejero-Sagastizabal, Galadriel IR - Pellejero-Sagastizabal G IRAD- Infectious Diseases Department, Hospital San Pedro, La Rioja, Spain. Electronic address: gpellejerosagas@gmail.com. FIR - Tsiodras, Sotirios IR - Tsiodras S IRAD- 4th Department of Internal Medicine and Infectious Diseases, Attikon University Hospital, National and Kapodistrian University of Athens Medical School, Athens, Greece. Electronic address: sotirios.tsiodras@gmail.com. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:34 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:34 [entrez] AID - S1198-743X(26)00600-2 [pii] AID - 10.1016/j.cmi.2026.10.022 [doi] PST - aheadofprint SO - Clin Microbiol Infect. 2026 Oct 28:S1198-743X(26)00600-2. doi: 10.1016/j.cmi.2026.10.022. PMID- 42951105 OWN - NLM STAT- Publisher LR - 20261028 IS - 1898-4002 (Electronic) IS - 1896-1126 (Linking) DP - 2026 Oct 28 TI - Alterations in salivary inflammatory cytokine levels in Graves' disease patients with and without orbitopathy. LID - S1896-1126(26)00049-0 [pii] LID - 10.1016/j.advms.2026.10.009 [doi] AB - PURPOSE: Graves' disease (GD) is an autoimmune thyroid disorder that may be complicated by Graves' orbitopathy (GO). This study evaluated salivary cytokine alterations in GD and the influence of orbitopathy activity and treatment, aiming to identify potential non-invasive biomarkers differentiating GD patients with and without orbitopathy from healthy controls. PATIENTS AND METHODS: This exploratory observational cross-sectional study included consecutively enrolled adult patients with confirmed GD, with or without orbitopathy (n=32 and n=18, respectively), and age- and gender-matched healthy controls (n=50). Selected salivary inflammatory cytokines were analysed. RESULTS: Salivary IL-10 and CCL2 concentrations were lower in GD patients without orbitopathy than in GD patients with orbitopathy and controls, although differences were attenuated after adjustment for demographic and clinical covariates. ROC analysis showed significant discrimination of GD without orbitopathy from controls for IL-10 (AUC=0.741), CCL2 (AUC=0.717), and IL-33 (AUC=0.678), and from GD with orbitopathy for IL-10 (AUC=0.755), CCL2 (AUC=0.740), and IL-33 (AUC=0.695). Higher IL-6 was observed in GD patients with orbitopathy receiving antithyroid drugs and higher IL-15 in patients with active GO, but these differences did not remain significant after multiple-comparison correction. CONCLUSIONS: Salivary cytokine patterns differed across the GD spectrum, with lower IL-10 and CCL2 concentrations in GD without clinically evident orbitopathy. IL-10, CCL2, and IL-33 showed apparent discriminatory ability in distinguishing GD from patients with orbitopathy and controls, warranting validation in independent cohorts. Subgroup findings for IL-6 and IL-15 provide insufficient evidence for treatment-related effects or GO activity markers. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Mlodzikowska, Martyna AU - Mlodzikowska M AD - Department of Conservative Dentistry, Poznan University of Medical Sciences, Poznan, Poland. FAU - Nijakowski, Kacper AU - Nijakowski K AD - Department of Conservative Dentistry, Poznan University of Medical Sciences, Poznan, Poland. Electronic address: kacpernijakowski@ump.edu.pl. FAU - Laganowski, Kacper AU - Laganowski K AD - Department of Conservative Dentistry, Poznan University of Medical Sciences, Poznan, Poland. FAU - Gruszczynski, Dawid AU - Gruszczynski D AD - Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, Poznan, Poland. FAU - Zawalna, Natalia AU - Zawalna N AD - Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, Poznan, Poland. FAU - Ruchala, Marek AU - Ruchala M AD - Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, Poznan, Poland. FAU - Sawicka-Gutaj, Nadia AU - Sawicka-Gutaj N AD - Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, Poznan, Poland. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Adv Med Sci JT - Advances in medical sciences JID - 101276222 SB - IM OTO - NOTNLM OT - Graves' disease OT - Graves' orbitopathy OT - biomarkers OT - cytokines OT - saliva COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:34 PHST- 2026/06/01 00:00 [received] PHST- 2026/08/20 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:34 [entrez] AID - S1896-1126(26)00049-0 [pii] AID - 10.1016/j.advms.2026.10.009 [doi] PST - aheadofprint SO - Adv Med Sci. 2026 Oct 28:S1896-1126(26)00049-0. doi: 10.1016/j.advms.2026.10.009. PMID- 42951104 OWN - NLM STAT- Publisher LR - 20261028 IS - 1877-0568 (Electronic) IS - 1877-0568 (Linking) DP - 2026 Oct 28 TI - Recovery of Pre-symptomatic Tegner Activity Level After Double-Level Osteotomy for Medial Compartment Osteoarthritis: Outcomes at 24 Months. PG - 104976 LID - S1877-0568(26)00397-X [pii] LID - 10.1016/j.otsr.2026.104976 [doi] AB - BACKGROUND: Double-level osteotomy (DLO) provides high return-to-sport rates, but these are generally based on the immediate preoperative activity level, which may already be reduced by knee pain. We evaluated recovery to a recalled pre-symptomatic activity level and associated factors. HYPOTHESIS: Recovery would be less frequent against the pre-symptomatic reference than the immediate preoperative level. PATIENTS AND METHODS: This retrospective study included 51 DLO patients wishing to resume sports, with symptom durations below five years. Tegner activity scale (TAS) scores were recalled for five years before surgery and assessed immediately preoperatively and at 12 and 24 months. Exploratory Firth logistic regression included age, BMI, Kellgren-Lawrence grade, and preoperative Pain Catastrophizing Scale (PCS) to examine failure to recover the five-year TAS at 24 months. RESULTS: Median [IQR] TAS was 5 [4-8] at the five-year reference, 3 [1-5] preoperatively, 4 [2-5] at 12 months, and 4 [3-7] at 24 months. At 24 months, 56.9% recovered the five-year TAS versus 88.2% recovering the preoperative TAS. Higher PCS was associated with failure to recover the five-year TAS (adjusted OR per point, 1.066; 95% CI, 1.008-1.143; p = 0.025). DISCUSSION: Recovery proportions differed by reference, with interpretation limited by recall bias and patient selection. Higher PCS was associated with non-recovery in exploratory analysis. LEVEL OF EVIDENCE: III; retrospective cohort study. CI - Copyright (c) 2026. Published by Elsevier Masson SAS. FAU - Nagashima, Ryo AU - Nagashima R AD - Department of Rehabilitation, Yokohama Sekishinkai Hospital, Japan. FAU - Miyazaki, Riku AU - Miyazaki R AD - Department of Rehabilitation, Yokohama Sekishinkai Hospital, Japan. FAU - Saito, Mizuki AU - Saito M AD - Department of Rehabilitation, Yokohama Sekishinkai Hospital, Japan. FAU - Goto, Kazuyuki AU - Goto K AD - Department of Rehabilitation, Yokohama Sekishinkai Hospital, Japan. FAU - Osawa, Katsunari AU - Osawa K AD - Department of Joint Surgery, Yokohama Sekishinkai Hospital, Japan. FAU - Yamada, Naoki AU - Yamada N AD - Department of Joint Surgery, Yokohama Sekishinkai Hospital, Japan. FAU - Hara, Jun AU - Hara J AD - Department of Orthopaedic Surgery, Yokohama Sekishinkai Hospital, Japan. FAU - Jung, Woon-Hwa AU - Jung WH AD - Department of Orthopaedic Surgery, Murup Hospital, Changwon, South Korea. FAU - Takeuchi, Ryohei AU - Takeuchi R AD - Department of Joint Surgery, Yokohama Sekishinkai Hospital, Japan. Electronic address: ryotake007@gmail.com. LA - eng PT - Journal Article DEP - 20261028 PL - France TA - Orthop Traumatol Surg Res JT - Orthopaedics & traumatology, surgery & research : OTSR JID - 101494830 SB - IM OTO - NOTNLM OT - Double-level osteotomy OT - Knee osteoarthritis OT - Pain catastrophizing OT - Pre-symptomatic activity level OT - Tegner Activity Scale COIS- Declaration of competing interest None. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:34 PHST- 2026/07/20 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:34 [entrez] AID - S1877-0568(26)00397-X [pii] AID - 10.1016/j.otsr.2026.104976 [doi] PST - aheadofprint SO - Orthop Traumatol Surg Res. 2026 Oct 28:104976. doi: 10.1016/j.otsr.2026.104976. PMID- 42951099 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-1635 (Electronic) IS - 1350-9462 (Linking) DP - 2026 Oct 28 TI - 3D Corneal and Retinal Bioprinting: Tissue Engineering Strategies, Translational Challenges, and Clinical Opportunities. PG - 101538 LID - S1350-9462(26)00104-7 [pii] LID - 10.1016/j.preteyeres.2026.101538 [doi] AB - Three-dimensional bioprinting has emerged as a promising strategy for addressing major unmet needs in ophthalmology, including the global shortage of donor corneal tissue and the absence of restorative therapies for retinal degenerative disease. Advances in biomaterials, stem cell biology, and biofabrication have enabled increasingly sophisticated ocular constructs, ranging from engineered corneal grafts to multicellular retinal models that recapitulate key aspects of native tissue architecture. However, substantial challenges remain in reproducing the optical, mechanical, and biological functions required for clinical translation. This review critically evaluates the current state of corneal and retinal bioprinting through a framework organized around tissue target, fabrication modality, and intended application. Major bioprinting approaches, including extrusion-based, laser-assisted, drop-on-demand, and light-based technologies, are compared with respect to resolution, cell compatibility, scalability, and translational potential. Particular emphasis is placed on the engineering constraints governing construct fidelity, bioink design, tissue integration, and long-term functional performance. Current evidence suggests that corneal and retinal bioprinting have progressed along distinct translational trajectories. Corneal bioprinting has achieved encouraging preclinical outcomes, with selected approaches entering early clinical evaluation, whereas retinal bioprinting has generated highly informative disease models but remains at an earlier stage of translational development. Across both tissues, the field has increasingly shifted from reproducing individual cellular components toward reconstructing the biological interfaces and multicellular systems that underlie tissue function. Future progress will depend on establishing functional validation frameworks, reproducible manufacturing workflows, and standardized benchmarks to support the clinical development of vision-restoring bioprinted constructs. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Shah, Jainam AU - Shah J AD - Albert Einstein College of Medicine, Bronx, New York, United States. FAU - Pathuri, Sachin AU - Pathuri S AD - Albert Einstein College of Medicine, Bronx, New York, United States. FAU - Sharma, Ruchi AU - Sharma R AD - National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States. FAU - Skottman, Heli AU - Skottman H AD - Eye Regeneration Group, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland. FAU - Moro, Anni AU - Moro A AD - Eye Regeneration Group, Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland. FAU - Karamichos, Dimitrios AU - Karamichos D AD - North Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, Texas, United States; Department of Family Medicine, Texas College of Osteopathic Medicine, Fort Worth, Texas, United States. FAU - Choi, Alexander AU - Choi A AD - North Texas Eye Research Institute, University of North Texas Health Science Center, Fort Worth, Texas, United States; Department of Family Medicine, Texas College of Osteopathic Medicine, Fort Worth, Texas, United States. FAU - Ong, Joshua AU - Ong J AD - Department of Ophthalmology, Harvard Medical School, Massachusetts Eye and Ear, Boston, Massachusetts, United States. FAU - Mehta, Jodhbir Singh AU - Mehta JS AD - Tissue Engineering and Stem Cell Group, Singapore Eye Research Institute, Singapore; Regenerative Therapy Research Group, Singapore Eye Research Institute, Singapore; Ophthalmology and Visual Sciences Academic Clinical Program, Duke-NUS Medical School, Singapore. FAU - Shrivastava, Anurag AU - Shrivastava A AD - Albert Einstein College of Medicine, Bronx, New York, United States; Department of Ophthalmology and Visual Sciences, Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York, United States. FAU - Sagoo, Mandeep AU - Sagoo M AD - National Institute for Health Research Biomedical Research Center at Moorfields Eye Hospital and UCL Institute of Ophthalmology, London, United Kingdom; Retinoblastoma Service, The Royal London Hospital, Barts Health NHS Trust, London, United Kingdom; Ocular Oncology Service, Moorfields Eye Hospital NHS Foundation Trust, London, United Kingdom. FAU - Berdahl, John P AU - Berdahl JP AD - Vance Thompson Vision, Sioux Falls, South Dakota, United States. FAU - Pandit, Rahul AU - Pandit R AD - Department of Ophthalmology, Blanton Eye Institute, Houston Methodist Hospital, Houston, Texas, United States; The Houston Methodist Research Institute, Houston Methodist Hospital, Houston, Texas, United States; Department of Ophthalmology, University of Texas Medical Branch, Galveston, Texas, United States. FAU - Bharti, Kapil AU - Bharti K AD - National Eye Institute, National Institutes of Health, Bethesda, Maryland, United States. Electronic address: kapil.bharti@nih.gov. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - Prog Retin Eye Res JT - Progress in retinal and eye research JID - 9431859 SB - IM COIS- Declaration of Competing Interest The authors declare no conflicts of interest relevant to the subject matter of this manuscript. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:34 PHST- 2026/08/14 00:00 [received] PHST- 2026/10/26 00:00 [revised] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:34 [entrez] AID - S1350-9462(26)00104-7 [pii] AID - 10.1016/j.preteyeres.2026.101538 [doi] PST - aheadofprint SO - Prog Retin Eye Res. 2026 Oct 28:101538. doi: 10.1016/j.preteyeres.2026.101538. PMID- 42951098 OWN - NLM STAT- Publisher LR - 20261028 IS - 1090-2449 (Electronic) IS - 0014-4894 (Linking) DP - 2026 Oct 28 TI - Thiosemicarbazone Derivatives Exhibit Potential Leishmanicidal Effects against L. infantum. PG - 109227 LID - S0014-4894(26)00134-7 [pii] LID - 10.1016/j.exppara.2026.109227 [doi] AB - Visceral leishmaniasis remains a significant global health burden, with current therapeutic options hampered by high toxicity and the emergence of drug-resistant strains. Thiosemicarbazones, a class of compounds with well-established antimicrobial and antiparasitic properties, represent a promising avenue for novel therapeutics. This study screened 18 thiosemicarbazone derivatives against Leishmania infantum and identified two lead candidates, TBD and TBB, with potent antileishmanial activity. TBD and TBB inhibited promastigotes (IC50 = 5.43 muM and 3.14 muM, respectively) and axenic amastigotes (EC50 = 8.22 muM and 16.11 muM), with favorable selectivity indices in human peripheral blood mononuclear cells (SI = 51.23 for TBD; SI = 9.58 for TBB). Cell death assays revealed that TBD induced dose-dependent apoptosis in promastigotes, with late-apoptotic populations increasing from 5.75% at 1x IC50 to 33.6% at 4x IC50. In axenic amastigotes, TBD at 4x EC50 triggered early apoptosis in 31.0% of cells, significantly surpassing amphotericin B (20.1%, p < 0.01). Furthermore, TBD induced mitochondrial membrane depolarization in L. infantum promastigotes, with Variation Index values of -0.451, -0.444, and -0.805 at 1x, 2x, and 4x IC(50), respectively. Importantly, TBD exhibited no detectable cytotoxicity in human PBMCs. These findings position TBD as a compelling candidate for further preclinical development as a novel treatment for visceral leishmaniasis. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Lamec, Delva Thyares Fonseca AU - Lamec DTF AD - Immunology of Infectious Diseases Laboratory, Department of Cellular and Molecular Biology, Federal University of Paraiba, Joao Pessoa 58051-900, PB, Brazil; Postgraduate Program in Natural and Synthetic Bioactive Products, Federal University of Paraiba, Joao Pessoa 58051-900, PB, Brazil. FAU - Fernandes, Ana Leticia Monteiro AU - Fernandes ALM AD - Immunology of Infectious Diseases Laboratory, Department of Cellular and Molecular Biology, Federal University of Paraiba, Joao Pessoa 58051-900, PB, Brazil. FAU - Cardoso, Leonardo Lima AU - Cardoso LL AD - Immunology of Infectious Diseases Laboratory, Department of Cellular and Molecular Biology, Federal University of Paraiba, Joao Pessoa 58051-900, PB, Brazil. FAU - Almeida, Fernanda Silva AU - Almeida FS AD - Immunology of Infectious Diseases Laboratory, Department of Cellular and Molecular Biology, Federal University of Paraiba, Joao Pessoa 58051-900, PB, Brazil. FAU - Ferreira, Rosalia Santos AU - Ferreira RS AD - Immunology of Infectious Diseases Laboratory, Department of Cellular and Molecular Biology, Federal University of Paraiba, Joao Pessoa 58051-900, PB, Brazil. FAU - Vanderley, Shayenne Eduarda Ramos AU - Vanderley SER AD - Immunology of Infectious Diseases Laboratory, Department of Cellular and Molecular Biology, Federal University of Paraiba, Joao Pessoa 58051-900, PB, Brazil; Postgraduate Program in Natural and Synthetic Bioactive Products, Federal University of Paraiba, Joao Pessoa 58051-900, PB, Brazil. FAU - Peixoto, Rephany Fonseca AU - Peixoto RF AD - Immunology of Infectious Diseases Laboratory, Department of Cellular and Molecular Biology, Federal University of Paraiba, Joao Pessoa 58051-900, PB, Brazil. FAU - do Amaral, Ian P G AU - do Amaral IPG AD - Laboratory of Biochemistry, Department of Cellular and Molecular Biology, Federal University of Paraiba, Joao Pessoa 58051-900, PB, Brazil. FAU - Castro, Aleff Cruz AU - Castro AC AD - Medicinal Organic Synthesis Laboratory, Department of Chemistry, Federal University of Paraiba, Joao Pessoa 58051-900, PB, Brazil. FAU - Lima Junior, Claudio Gabriel AU - Lima Junior CG AD - Medicinal Organic Synthesis Laboratory, Department of Chemistry, Federal University of Paraiba, Joao Pessoa 58051-900, PB, Brazil. FAU - Keesen, Tatjana Souza Lima Clemente AU - Keesen TSLC AD - Immunology of Infectious Diseases Laboratory, Department of Cellular and Molecular Biology, Federal University of Paraiba, Joao Pessoa 58051-900, PB, Brazil. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Exp Parasitol JT - Experimental parasitology JID - 0370713 SB - IM OTO - NOTNLM OT - Leishmaniasis OT - antileishmanial compounds OT - low cytotoxicity OT - systemic toxicity COIS- Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:34 PHST- 2025/08/18 00:00 [received] PHST- 2026/10/21 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:34 [entrez] AID - S0014-4894(26)00134-7 [pii] AID - 10.1016/j.exppara.2026.109227 [doi] PST - aheadofprint SO - Exp Parasitol. 2026 Oct 28:109227. doi: 10.1016/j.exppara.2026.109227. PMID- 42951097 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-3700 (Electronic) IS - 0031-9422 (Linking) DP - 2026 Oct 28 TI - Coelawrenins A-K, dihydrophenanthrene and stilbene derivatives from Coelogyne lawrenceana and their anti-cancer activity against glioma cells. PG - 115126 LID - S0031-9422(26)00359-6 [pii] LID - 10.1016/j.phytochem.2026.115126 [doi] AB - Eleven previously undescribed compounds, designated coelawrenins A-K (1-11), together with seventeen known compounds (12-28), were isolated from the whole plant of orchid Coelogyne lawrenceana. The structures of the previously undescribed compounds were elucidated by comprehensive spectroscopic analyses, including NMR, MS, UV, and FT-IR, and their configurations were determined by optical rotation and circular dichroism. In vitro cytotoxicity of isolated compounds (5, 7-10, 12-17, 19-21, and 23-27) towards glioma cell lines was examined by the MTT assay. Among the isolated constituents, compounds 9, 10, and 12 exhibited moderate to potent cytotoxicity against glioma cell lines. Specifically, coelawrenin J (10) demonstrated significant inhibitory effects with an IC(50) value of 55.20 +/- 2.34 (U87MG), 42.40 +/- 5.29 (U251MG), and 57.77 +/- 4.25 (patient-derived Jx22) muM. Importantly, coelawrenin J (10) showed lower toxicity toward normal human dermal papilla cells, indicating selective activity against malignant glioma cells. Consistent with its antiproliferative effects, coelawrenin J (10) dose-dependently suppressed clonogenic survival, suggesting sustained inhibition of tumorigenic potential. Mechanistic investigation revealed significant downregulation of the anti-apoptotic proteins Bcl-xL and Mcl-1, indicating induction of apoptotic priming and potential sensitization to therapeutic intervention. Collectively, these findings identify coelawrenin J (10) as a selective pro-apoptotic natural product with promising translational potential for glioblastoma therapy, warranting further preclinical investigation. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Rojpitikul, Thanawan AU - Rojpitikul T AD - Pharmaceutical Sciences and Technology Program, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand. FAU - Aung, Hnin Mon AU - Aung HM AD - Pharmaceutical Sciences and Technology Program, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand. FAU - Naini, Al Arofatus AU - Naini AA AD - Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), Cibinong Science Center Complex - BRIN, Cibinong, Bogor, West Java, 16911, Indonesia. FAU - Punpreuk, Yanyong AU - Punpreuk Y AD - Department of Agriculture, Ministry of Agriculture and Cooperatives, Bangkok, 10900, Thailand. FAU - Jungsuttiwong, Siriporn AU - Jungsuttiwong S AD - Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani, 34190, Thailand. FAU - Kongkatitham, Virunh AU - Kongkatitham V AD - Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand. FAU - Rojsitthisak, Pornchai AU - Rojsitthisak P AD - Department of Food and Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Center of Excellence in Natural Products for Ageing and Chronic Diseases, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand. FAU - Griguer, Corinne E AU - Griguer CE AD - Free Radical & Radiation Biology Program, Department of Radiation Oncology, University of Iowa, Iowa, IA 52242, USA. FAU - Buranasudja, Visarut AU - Buranasudja V AD - Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand. FAU - Sritularak, Boonchoo AU - Sritularak B AD - Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Center of Excellence in Natural Products for Ageing and Chronic Diseases, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand. Electronic address: boonchoo.sr@chula.ac.th. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Phytochemistry JT - Phytochemistry JID - 0151434 SB - IM OTO - NOTNLM OT - Anti-cancer OT - Coelogyne lawrenceana OT - Dihydrophenanthrene OT - Glioblastoma OT - Orchidaceae OT - Stilbene COIS- Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:34 PHST- 2026/03/06 00:00 [received] PHST- 2026/10/22 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:34 [entrez] AID - S0031-9422(26)00359-6 [pii] AID - 10.1016/j.phytochem.2026.115126 [doi] PST - aheadofprint SO - Phytochemistry. 2026 Oct 28:115126. doi: 10.1016/j.phytochem.2026.115126. PMID- 42951092 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-3169 (Electronic) IS - 0378-4274 (Linking) DP - 2026 Oct 28 TI - A single exposure to the environmental contaminant tributyltin induces oxidative stress and vascular endothelial dysfunction. PG - 113225 LID - S0378-4274(26)02454-9 [pii] LID - 10.1016/j.toxlet.2026.113225 [doi] AB - Organotin compounds, such as tributyltin (TBT), are environmental contaminants known for toxic effects. Subacute exposure to TBT leads to vascular dysfunction associated with increased production of reactive oxygen species (ROS). This study aimed to investigate whether a single exposure to TBT induces endothelial dysfunction. Adult female rats were divided into two groups: control (CT) and TBT-exposed. Vascular reactivity of isolated aortic rings was assessed 24hours after a single oral dose of TBT (500ng/kg). Vasodilator responses to acetylcholine and sodium nitroprusside were not altered by TBT exposure. However, aortic rings from TBT-exposed rats exhibited greater contractile response to phenylephrine and reduced influence of endothelial denudation or L-NAME incubation on vascular reactivity. The impaired nitrergic modulation induced by TBT was prevented by co-incubation with superoxide dismutase plus L-NAME. In vitro DAF-2 assay indicated reduced NO production in the TBT group. TBT-induced vascular hyperreactivity was reversed by incubation with apocynin, TIRON, catalase, allopurinol, losartan, indomethacin, NS-398, SQ-29,548, and SC-19,220. In contrast, the non-selective NOX inhibitor VAS2870 did not modify the response to phenylephrine. Dihydroethidium staining showed increased ROS levels- including superoxide anion and hydrogen peroxide-in aortas from TBT-exposed animals. Scanning electron microscopy revealed endothelial structural damage in the TBT group. Together, these findings indicate that a single dose of TBT (500ng/kg) caused vascular dysfunction, likely resulting from impaired endothelial and nitrergic modulation mediated by increased ROS production derived from xanthine oxidase, activation of the local renin-angiotensin system, and cyclooxygenase pathways in female rats. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Marques, Vinicius Bermond AU - Marques VB AD - Department of Physiology, Health Sciences Center, Federal University of Espirito Santo, Brazil. Electronic address: ivanita.stefanon@ufes.br. FAU - Santos, Gersica de Almeida Correia AU - Santos GAC AD - Department of Physiology, Health Sciences Center, Federal University of Espirito Santo, Brazil. FAU - Silva, Kevin Mesquita AU - Silva KM AD - Department of Physiology, Health Sciences Center, Federal University of Espirito Santo, Brazil. FAU - Ximenes, Carolina Falcao AU - Ximenes CF AD - Department of Physiology, Health Sciences Center, Federal University of Espirito Santo, Brazil. FAU - Costa, Anna Karolina N AU - Costa AKN AD - Department of Physiology, Health Sciences Center, Federal University of Espirito Santo, Brazil. FAU - Hortelan, Michelle AU - Hortelan M AD - Department of Physiology, Health Sciences Center, Federal University of Espirito Santo, Brazil. FAU - Taufner, Sara Bianca AU - Taufner SB AD - Department of Physiology, Health Sciences Center, Federal University of Espirito Santo, Brazil. FAU - Graceli, Jones Bernardes AU - Graceli JB AD - Department of Morphology, Health Sciences Center, Federal University of Espirito Santo, Brazil; Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL 62901, USA. FAU - Fernandes, Aurelia Araujo AU - Fernandes AA AD - Department of Morphology, Health Sciences Center, Federal University of Espirito Santo, Brazil. FAU - Ribeiro, Eduardo Hertel AU - Ribeiro EH AD - Department of Physiology, Health Sciences Center, Federal University of Espirito Santo, Brazil. FAU - Dedkova, Elena N AU - Dedkova EN AD - Department of Molecular Biosciences, University of California, Davis, CA, USA; Department of Basic Sciences, California Northstate University, Elk Grove, CA, USA. FAU - Stefanon, Ivanita AU - Stefanon I AD - Department of Physiology, Health Sciences Center, Federal University of Espirito Santo, Brazil. Electronic address: bermondmarques@gmail.com. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Toxicol Lett JT - Toxicology letters JID - 7709027 SB - IM OTO - NOTNLM OT - aortic rings OT - endothelium OT - environmental contaminant OT - oxidative stress OT - tributyltin OT - vascular reactivity COIS- Declaration of Competing Interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:33 PHST- 2026/07/13 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:33 [entrez] AID - S0378-4274(26)02454-9 [pii] AID - 10.1016/j.toxlet.2026.113225 [doi] PST - aheadofprint SO - Toxicol Lett. 2026 Oct 28:113225. doi: 10.1016/j.toxlet.2026.113225. PMID- 42951088 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-6513 (Electronic) IS - 0885-3924 (Linking) DP - 2026 Oct 28 TI - Models of palliative care for malignant disease in rural settings: Systematic review. LID - S0885-3924(26)01027-4 [pii] LID - 10.1016/j.jpainsymman.2026.10.003 [doi] AB - BACKGROUND: Access to palliative care for people with advanced cancer in rural settings remains inequitable due to workforce shortages, geographic isolation, and limited formalised care structures. This review synthesised evidence on rural palliative care models for people with malignant disease. METHOD: Systematic review of rural palliative care models for malignant disease published since January 2000; four databases were searched on 30 May 2024 and updated 30 May 2026. Predefined domains included: model/population characteristics, Health related quality of life (HRQoL), symptom burden, healthcare utilisation, model evaluation/experience, and health professional education. Study quality was appraised and findings synthesised narratively. RESULTS: 22 publications representing 18 studies were included. Models varied in leadership and delivery and were predominantly community-based (13/18); nine were specialist palliative care-led, six oncology-, generalist- or primary care-led, and three lay navigator-led with specialist support. Telehealth or hybrid delivery was used in 11/18. Outcomes were heterogeneous. Symptom burden (6/18) and location of death (5/18) were commonly reported. Several specialist- and generalist-led observational studies reported improvements in selected symptoms and health care use, while the two randomised controlled trials showed mixed HRQoL findings. Model acceptability and experience were frequently assessed (15/18), with continuity, flexible delivery, telehealth support and workforce development commonly valued, although infrastructure and resource constraints moderated implementation. CONCLUSION: Rural palliative care models may support symptom management, access and care closer to home, with specialist palliative care expertise commonly embedded within local services. Comparative and economic evaluation of scalable rural models remains limited. PROTOCOL REGISTRATION: PROSPERO CRD42022303861. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Arnold, Kirsten AU - Arnold K AD - Monash University, Melbourne. FAU - Pascoe, Amy AU - Pascoe A AD - Monash University, Melbourne. FAU - Steele, Patrick AU - Steele P AD - Dept of Palliative Care, Royal Melbourne Hospital, Melbourne; Dept of Palliative Care, Peter MacCallum Cancer Centre, Melbourne, Australia; Palliative Care South East, Narre Warren, Australia. FAU - Hon, Venise AU - Hon V AD - Monash University, Melbourne. FAU - Mounsey, Lisa AU - Mounsey L AD - Dept of Palliative Care, Barwon health, Geelong. FAU - Smallwood, Natasha AU - Smallwood N AD - Monash University, Melbourne; Department of Respiratory Medicine, Alfred Health, Melbourne. FAU - Disler, Rebecca AU - Disler R AD - Monash University, Melbourne; Department of Rural Health, The Melbourne University. Electronic address: Rebecca.disler@monash.edu. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - J Pain Symptom Manage JT - Journal of pain and symptom management JID - 8605836 SB - IM COIS- Conflicts of interest None EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:33 PHST- 2026/07/08 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:33 [entrez] AID - S0885-3924(26)01027-4 [pii] AID - 10.1016/j.jpainsymman.2026.10.003 [doi] PST - aheadofprint SO - J Pain Symptom Manage. 2026 Oct 28:S0885-3924(26)01027-4. doi: 10.1016/j.jpainsymman.2026.10.003. PMID- 42951081 OWN - NLM STAT- Publisher LR - 20261028 IS - 0219-1032 (Electronic) IS - 1016-8478 (Linking) DP - 2026 Oct 28 TI - A New Chapter for Molecules and Cells. PG - 100409 LID - S1016-8478(26)00100-7 [pii] LID - 10.1016/j.mocell.2026.100409 [doi] AB - This editorial closes a three-year editorial term at Molecules and Cells and reviews what changed during that period, including more selective editorial standards, the transition to publication by Elsevier, the introduction of new article formats, and a broadening of the journal's international authorship and readership. The journal enters its next chapter with stronger visibility and a clear editorial direction. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Inc. All rights reserved. FAU - Lee, Seung-Jae V AU - Lee SV AD - Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, South Korea. Electronic address: seungjaevlee@kaist.ac.kr. LA - eng PT - Editorial DEP - 20261028 PL - United States TA - Mol Cells JT - Molecules and cells JID - 9610936 SB - IM COIS- Declaration of conflict of interest S.-J.V.L. served as Editor-in-Chief of Molecules and Cells during the period described. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:33 PHST- 2026/10/23 00:00 [received] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:33 [entrez] AID - S1016-8478(26)00100-7 [pii] AID - 10.1016/j.mocell.2026.100409 [doi] PST - aheadofprint SO - Mol Cells. 2026 Oct 28:100409. doi: 10.1016/j.mocell.2026.100409. PMID- 42951080 OWN - NLM STAT- Publisher LR - 20261028 IS - 2589-5559 (Electronic) IS - 2589-5559 (Linking) DP - 2026 Oct 28 TI - Postmenopausal estradiol levels and female sex modulate haemodynamic responsiveness in experimental portal hypertension. PG - 102073 LID - S2589-5559(26)00344-7 [pii] LID - 10.1016/j.jhepr.2026.102073 [doi] AB - BACKGROUND & AIMS: Sex and 17beta-estradiol (E2) influence outcomes of cirrhosis, yet preclinical studies in portal hypertension are conducted predominantly in males. We investigated sex and E2-dependent differences in G protein-coupled estrogen receptor 1 (GPER1) signalling and portal pressure response to PDE5 inhibition (PDE5i) in experimental cirrhosis. METHODS: We assessed sex inclusion in rodent studies by PubMed literature search and analysed human hepatic GPER1 expression by sex and age using bulk RNA-seq. Bile duct ligation was performed in male, ovariectomised (OVX) and sham-operated female rats. E2 was administered 24h before invasive haemodynamic assessment in males and OVX females. Acute PDE5i effects were investigated during haemodynamic measurement. RESULTS: Of 510 rat and 1,657 mouse studies, 87.3% and 77.3% respectively included males only. Human bulk RNA-seq suggested lower hepatic GPER1 expression in cirrhotic females aged >/= 50 vs. < 50 years. OVX reduced hepatic - but not aortic - GPER1 expression. E2 reduced portal pressure (mean +/- SEM; 22.6 +/- 1.4 vs. 14.3 +/- 1.1 mmHg, p = 0.009) by lowering hepatic portal vascular resistance (74.4 +/- 12.7 vs. 25.4 +/- 3.1 mmHg.min.ml(-1).g(-1), p = 0.03) only in OVX females. PDE5i decreased portal pressure in males (21.3 +/- 1.2 vs. 16.9 +/- 1.8 mmHg, p = 0.03) irrespective of E2. OVX abolished the PDE5i response, which was restored by E2 (14.3 +/- 1.1 vs. 10.0 +/- 0.9 mmHg, p = 0.04). CONCLUSIONS: In experimental cirrhosis, estradiol depletion in OVX females was associated with reduced hepatic GPER1 expression and a diminished portal pressure response to PDE5i. This highlights sex and menopausal status as key determinants of treatment response in portal hypertension and supports the need for sex-specific validation. IMPACT AND IMPLICATIONS: Sex and hormonal status likely contribute to clinically relevant variability in the pharmacology of portal hypertension. Our work highlights that haemodynamic drug response varies due to sex and estradiol status in pre- and postmenopausal females, yet preclinical studies predominantly rely on male models. Integrating sex and menopausal status into early drug development could improve translational accuracy and anticipate non-responder populations. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Schachteli, Fabian AU - Schachteli F AD - Department of Internal Medicine B, University of Munster, Munster, Germany. Electronic address: fabian.schachteli@ukmuenster.de. FAU - Klein, Sabine AU - Klein S AD - Department of Internal Medicine B, University of Munster, Munster, Germany. Electronic address: sabine.klein@ukmuenster.de. FAU - Schierwagen, Robert AU - Schierwagen R AD - Department of Internal Medicine B, University of Munster, Munster, Germany. Electronic address: robert.schierwagen@ukmuenster.de. FAU - Fischer, Julia AU - Fischer J AD - Department of Internal Medicine B, University of Munster, Munster, Germany. Electronic address: Julia.Fischer2@ukmuenster.de. FAU - Peiffer, Kai-Henrik AU - Peiffer KH AD - Department of Internal Medicine B, University of Munster, Munster, Germany. Electronic address: kai-henrik.peiffer@ukmuenster.de. FAU - Fobker, Manfred AU - Fobker M AD - Central Laboratory Facility, University Hospital Munster, Munster, Germany. Electronic address: manfred.fobker@ukmuenster.de. FAU - Vorona, Elena AU - Vorona E AD - Department of Internal Medicine B, University of Munster, Munster, Germany. Electronic address: elena.vorona@ukmuenster.de. FAU - Arif, Sania AU - Arif S AD - Department of Internal Medicine B, University of Munster, Munster, Germany. Electronic address: sania.arif@ukmuenster.de. FAU - Zarei, Sara AU - Zarei S AD - Department of Internal Medicine B, University of Munster, Munster, Germany. Electronic address: sara.zarei@ukmuenster.de. FAU - Brol, Maximilian Joseph AU - Brol MJ AD - Department of Internal Medicine B, University of Munster, Munster, Germany. Electronic address: maximilian.brol@ukmuenster.de. FAU - Trebicka, Jonel AU - Trebicka J AD - Department of Internal Medicine B, University of Munster, Munster, Germany; European Foundation for the Study of Chronic Liver Failure, Barcelona, Spain. Electronic address: jonel.trebicka@ukmuenster.de. FAU - Uschner, Frank Erhard AU - Uschner FE AD - Department of Internal Medicine B, University of Munster, Munster, Germany. Electronic address: frankerhard.uschner@ukmuenster.de. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - JHEP Rep JT - JHEP reports : innovation in hepatology JID - 101761237 SB - IM OTO - NOTNLM OT - estradiol signalling OT - liver cirrhosis OT - menopause OT - portal hypertension OT - sex differences OT - vascular dysfunction COIS- Declaration of Competing Interest: The authors declare no conflict of interest that could potentially influence or bias the work. F.E.U. received speaker fees from AstraZeneca, Dr. Falk Foundation, AbbVie, Gilead and travel support from AbbVie. J.T. was supported by German Research Foundation (DFG) project ID 403224013-SFB 1382 (A09), by the German Federal Ministry of Education and Research (BMBF) for the DEEP-HCC project and by the Hessian Ministry of Higher Education, Research and the Arts (HMWK) for the ENABLE and ACLF-I cluster projects. J.T. is supported by the MICROB-PREDICT (project ID 825694), DECISION (project ID 847949), and IHMCSA (project ID 964590) projects have received funding from the European Union's Horizon 2020 research and innovation program and received speaker and/or consulting fees from AstraZeneca, Versantis (a part of GENFIT Group), Gore, Boehringer-Ingelheim, Falk, Grifols, Genfit and CSL Behring. F.S., S.K., R.S., K.H.P., M.F., E.V., S.A., S.Z., J.F., M.J.B. have nothing to declare. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:33 PHST- 2026/03/19 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:33 [entrez] AID - S2589-5559(26)00344-7 [pii] AID - 10.1016/j.jhepr.2026.102073 [doi] PST - aheadofprint SO - JHEP Rep. 2026 Oct 28:102073. doi: 10.1016/j.jhepr.2026.102073. PMID- 42951079 OWN - NLM STAT- Publisher LR - 20261028 IS - 2589-5559 (Electronic) IS - 2589-5559 (Linking) DP - 2026 Oct 28 TI - A protein modification of TIGAR Fuels HCC Growth by Boosting Tumor Glucose Metabolism. PG - 102084 LID - S2589-5559(26)00355-1 [pii] LID - 10.1016/j.jhepr.2026.102084 [doi] AB - BACKGROUND & AIMS: Hepatocellular carcinoma (HCC) is the most common primary liver malignancy and is characterized by profound metabolic reprogramming. TIGAR (TP53-induced glycolysis and apoptosis regulator) regulates glucose metabolism and redox balance by redirecting glucose flux toward the pentose phosphate pathway. Although TIGAR is frequently upregulated in HCC, the mechanisms controlling its activity remain poorly understood. We investigated whether SUMOylation regulates TIGAR function and HCC progression. METHODS: TIGAR SUMOylation was evaluated in two independent HCC patient cohorts (n=86 and n=19), HCC cell lines and mouse models. Seahorse assays, metabolomic profiling, (13)C-glucose tracing, mitochondrial analyses and in vivo tumor models were used to investigate its effects on glucose metabolism, oxidative stress and tumor growth. RESULTS: TIGAR was found to be SUMOylated in HCC tissues, cultured cells and murine tumor models. SUMOylation enhanced the pro-tumorigenic activity of TIGAR by promoting its interaction with glycolytic enzymes, including PFKFB3 and HK2, resulting in increased glycolytic activity and pentose phosphate pathway flux. Conversely, SUMOylation-deficient K82R cells exhibited increased oxidative stress, mitochondrial dysfunction and reduced proliferation and invasion compared with WT TIGAR. In the orthotopic liver model (n=4 per group), K82R expression was associated with reduced tumor growth. In the CAM assay, SUMOylation-deficient K82R clones showed significantly reduced tumor weight (n=8 per group, p < 0.0001) and volume (n=4 per group, p < 0.05) compared with WT TIGAR cells. CONCLUSIONS: This study identifies Lys82 SUMOylation as a novel mechanism regulating TIGAR-mediated metabolic reprogramming and HCC progression. Targeting TIGAR SUMOylation may represent a novel therapeutic strategy for HCC. IMPACT AND IMPLICATIONS: This study identifies TIGAR SUMOylation as a new mechanism driving metabolic adaptation in hepatocellular carcinoma. SUMOylated TIGAR promotes glycolysis, pentose phosphate pathway activity and mitochondrial function. Loss of TIGAR SUMOylation increases oxidative stress and reduces tumor growth and invasion. These findings highlight TIGAR SUMOylation as a potential biomarker and therapeutic target in liver cancer. Overall, our data support targeting this pathway to improve therapeutic strategies for hepatocellular carcinoma. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Rejano-Gordillo, Claudia M AU - Rejano-Gordillo CM AD - Liver Disease Lab, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Bizkaia, Spain. Electronic address: crejano@cicbigoune.es. FAU - Conter, Carolina AU - Conter C AD - Liver Disease Lab, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Bizkaia, Spain. FAU - Pena-Sanfelix, Patricia AU - Pena-Sanfelix P AD - Liver Disease Lab, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Bizkaia, Spain. FAU - Mosca, Laura AU - Mosca L AD - Department of Life Sciences, Health and Health Professions, Link Campus University, Via del Casale di S. Pio V, 44, 00165 Roma, Italy. FAU - Merlos-Rodrigo, Miguel Angel AU - Merlos-Rodrigo MA AD - Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. FAU - Tamargo-Azpilicueta, Joaquin AU - Tamargo-Azpilicueta J AD - Institute of Chemical Research (IIQ)-Isla de la Cartuja Scientific Research Center (cicCartuja), University of Seville-CSIC, Spain. FAU - Diaz-Cuevas, Carlos AU - Diaz-Cuevas C AD - Liver Disease Lab, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Bizkaia, Spain. FAU - Lachiondo-Ortega, Sofia AU - Lachiondo-Ortega S AD - Liver Disease Lab, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Bizkaia, Spain. FAU - Zapata-Pavas, L Estefania AU - Zapata-Pavas LE AD - Liver Disease Lab, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Bizkaia, Spain. FAU - Ferreiro, Nerea AU - Ferreiro N AD - Liver Disease Lab, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Bizkaia, Spain. FAU - Davoli, Eleonora AU - Davoli E AD - Liver Disease Lab, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Bizkaia, Spain. FAU - Gutiez, Naiara AU - Gutiez N AD - Inflammation and Macrophage Plasticity Lab, CIC bioGUNE-BRTA, Derio, Bizkaia, Spain. FAU - Anguita, Juan AU - Anguita J AD - Inflammation and Macrophage Plasticity Lab, CIC bioGUNE-BRTA, Derio, Bizkaia, Spain; Ikerbasque, Basque Foundation for Science, Bilbao, Spain. FAU - Piva, Marco AU - Piva M AD - Liver Disease Lab, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Bizkaia, Spain; Ikerbasque, Basque Foundation for Science, Bilbao, Spain. FAU - Guerra, Pietro AU - Guerra P AD - Department of Medicine, University of Padova, via Giustiniani, 2, 35128 Padova, Italy. FAU - Gonzalez-Recio, Irene AU - Gonzalez-Recio I AD - Liver Disease Lab, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Bizkaia, Spain. FAU - Goikoetxea-Usandizaga, Naroa AU - Goikoetxea-Usandizaga N AD - Liver Disease Lab, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Bizkaia, Spain; Biomedical Research Network Center for Liver and Digestive Diseases (CIBERehd), Carlos III National Health Institute, Madrid, Spain. FAU - Beltra, Marc AU - Beltra M AD - Department of Biochemistry and Molecular Biomedicine, Faculty of Biology, Institute of Biomedicine of the University of Barcelona, University of Barcelona, Barcelona, Spain; Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Barcelona, Spain. FAU - Azkargorta, Mikel AU - Azkargorta M AD - Proteomics Platform, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Derio, Spain. FAU - Elortza, Felix AU - Elortza F AD - Proteomics Platform, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), Derio, Spain. FAU - Herman Sanchez, Natalia AU - Herman Sanchez N AD - Department of Cell Biology, Physiology, and Immunology, University of Cordoba, CIBER Pathophysiology of Obesity and Nutrition (CIBERobn), Cordoba, 14004, Spain; Maimonides Institute of Biomedical Research of Cordoba (IMIBIC), Reina Sofia University Hospital, Cordoba, 14004, Spain. FAU - Pontisso, Patrizia AU - Pontisso P AD - Department of Medicine, University of Padova, via Giustiniani, 2, 35128 Padova, Italy. FAU - Banales, Jesus M AU - Banales JM AD - Ikerbasque, Basque Foundation for Science, Bilbao, Spain; Biomedical Research Network Center for Liver and Digestive Diseases (CIBERehd), Carlos III National Health Institute, Madrid, Spain; Department of Liver and Gastrointestinal Diseases, Biodonostia Health Research Institute, Donostia University Hospital, University of the Basque Country (UPV/EHU), San Sebastian, Spain; Department of Biochemistry and Genetics, School of Sciences, University of Navarra, Pamplona, Spain. FAU - Nogueiras, Ruben AU - Nogueiras R AD - Molecular Metabolism Lab, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), Av. Barcelona, Campus Vida, Santiago de Compostela University, Santiago de Compostela, Spain. FAU - Marin, Jose J G AU - Marin JJG AD - Experimental Hepatology and Drug Targeting (HEVEPHARM), Institute for Biomedical Research of Salamanca (IBSAL), University of Salamanca, 37007 Salamanca, Spain. FAU - Fernandez-Barrena, Maite G AU - Fernandez-Barrena MG AD - Biomedical Research Network Center for Liver and Digestive Diseases (CIBERehd), Carlos III National Health Institute, Madrid, Spain; Navarra Health Research Institute (IdiSNA), Hepatology Laboratory, Solid Tumors Program, CIMA, CCUN, University of Navarra, 31008 Pamplona, Spain. FAU - Avila, Matias A AU - Avila MA AD - Biomedical Research Network Center for Liver and Digestive Diseases (CIBERehd), Carlos III National Health Institute, Madrid, Spain; Navarra Health Research Institute (IdiSNA), Hepatology Laboratory, Solid Tumors Program, CIMA, CCUN, University of Navarra, 31008 Pamplona, Spain. FAU - Zorzano, Antonio AU - Zorzano A AD - Department of Biochemistry and Molecular Biomedicine, Faculty of Biology, Institute of Biomedicine of the University of Barcelona, University of Barcelona, Barcelona, Spain; Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Barcelona, Spain; Biomedical Research Network Center for Diabetes and Associated Metabolic Diseases (CIBERDEM), Carlos III Health Institute, 28029, Madrid, Spain. FAU - Luque, Raul M AU - Luque RM AD - Department of Cell Biology, Physiology, and Immunology, University of Cordoba, CIBER Pathophysiology of Obesity and Nutrition (CIBERobn), Cordoba, 14004, Spain; Maimonides Institute of Biomedical Research of Cordoba (IMIBIC), Reina Sofia University Hospital, Cordoba, 14004, Spain. FAU - Heger, Zbynek AU - Heger Z AD - Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic. FAU - Gahete, Manuel D AU - Gahete MD AD - Department of Cell Biology, Physiology, and Immunology, University of Cordoba, CIBER Pathophysiology of Obesity and Nutrition (CIBERobn), Cordoba, 14004, Spain; Maimonides Institute of Biomedical Research of Cordoba (IMIBIC), Reina Sofia University Hospital, Cordoba, 14004, Spain. FAU - Villa, Erica AU - Villa E AD - Chimomo Department, Gastroenterology Unit, University of Modena and Reggio Emilia, Modena, Italy; UC Gastroenterology, Department of Medical Specialties, Modena University Hospital, Modena, Italy. FAU - Lafarga, Miguel AU - Lafarga M AD - Departamento de Anatomia y Biologia Celular, Universidad de Cantabria, Santander, Spain. FAU - Diaz-Moreno, Irene AU - Diaz-Moreno I AD - Institute of Chemical Research (IIQ)-Isla de la Cartuja Scientific Research Center (cicCartuja), University of Seville-CSIC, Spain. FAU - Martinez-Chantar, Maria Luz AU - Martinez-Chantar ML AD - Liver Disease Lab, Center for Cooperative Research in Biosciences (CIC bioGUNE), Basque Research and Technology Alliance (BRTA), 48160 Derio, Bizkaia, Spain; Biomedical Research Network Center for Liver and Digestive Diseases (CIBERehd), Carlos III National Health Institute, Madrid, Spain. Electronic address: mlmartinez@cicbiogune.es. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - JHEP Rep JT - JHEP reports : innovation in hepatology JID - 101761237 SB - IM OTO - NOTNLM OT - HCC OT - SUMOylation OT - TIGAR OT - Warburg effect OT - metabolism OT - mitochondrial dysfunction OT - oxidative stress OT - pentose phosphate pathway COIS- CONFLICT OF INTEREST STATEMENT the authors declare no conflict of interest. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:33 PHST- 2025/12/10 00:00 [received] PHST- 2026/10/21 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:33 [entrez] AID - S2589-5559(26)00355-1 [pii] AID - 10.1016/j.jhepr.2026.102084 [doi] PST - aheadofprint SO - JHEP Rep. 2026 Oct 28:102084. doi: 10.1016/j.jhepr.2026.102084. PMID- 42951078 OWN - NLM STAT- Publisher LR - 20261028 IS - 2589-5559 (Electronic) IS - 2589-5559 (Linking) DP - 2026 Oct 28 TI - Myeloid Suppressor Cells Drive HCC Invasiveness via the THBS1-SDC4 Pathway. PG - 102085 LID - S2589-5559(26)00356-3 [pii] LID - 10.1016/j.jhepr.2026.102085 [doi] AB - BACKGROUND & AIMS: Monocytic myeloid-derived suppressor cells (M-MDSCs) accumulate in hepatocellular carcinoma (HCC), but their activation and tumor-promoting mechanisms remain unclear. We investigated how stromal-myeloid-tumor crosstalk promotes HCC invasiveness and epithelial-mesenchymal transition (EMT). METHODS: We analyzed single-cell RNA-sequencing data from 36 HCC patients and spatial transcriptomics data from 2 HCC specimens to characterize M-MDSC localization and intercellular signaling. Matrix cancer-associated fibroblast and M-MDSC phenotypes and interactions were assessed using molecular and functional assays. Thrombospondin-1-syndecan-4 signaling was investigated in xenografts in immunodeficient mice (n = 8/group) and diethylnitrosamine/carbon tetrachloride-induced HCC in the immunocompetent mice (n = 6/group). RESULTS: M-MDSCs preferentially localized to EMT-active tumor margins and correlated with EMT activity (Spearman r = 0.30; false discovery rate = 3.3 x 10(-4)) and shorter overall survival (log-rank P = 0.00124). Matrix cancer-associated fibroblasts promoted M-MDSC activation through collagen-associated CD44 signaling. Activated M-MDSCs induced EMT through thrombospondin-1-syndecan-4 signaling, whereas syndecan-4 deficiency suppressed tumor cell proliferation, migration, invasion, and EMT. Thrombospondin-1-deficient M-MDSCs reduced xenograft progression and increased interferon-gamma-positive CD8(+) T-cell infiltration and cytotoxic immune markers in immunocompetent HCC. A thrombospondin communication pathway score was associated with shorter overall survival (log-rank P = 2.28 x 10(-5); Cox hazard ratio = 1.23, P = 0.0193). CONCLUSIONS: Matrix cancer-associated fibroblasts promote monocytic myeloid-derived suppressor cell activation, driving epithelial-mesenchymal transition in hepatocellular carcinoma through thrombospondin-1-syndecan-4 signaling. Targeting this axis may restrain tumor progression and enhance antitumor immunity. IMPACT AND IMPLICATIONS: This study provides mechanistic insight into how M-MDSCs are activated within the HCC tumor microenvironment and subsequently drive tumor cell EMT through THBS1-SDC4 signaling. These findings highlight the importance of tumor-immune-stromal crosstalk in HCC progression and provide a mechanistic framework for understanding the pro-tumor functions of M-MDSCs. The THBS1-SDC4 axis may represent a therapeutically targetable pathway to restrain M-MDSC-mediated tumor aggressiveness and modulate the tumor microenvironment. Further validation in independent clinical cohorts and assessment of therapeutic efficacy are warranted to determine its translational potential. CI - Copyright (c) 2026 The Author(s). Published by Elsevier B.V. All rights reserved. FAU - Liu, Siyuan AU - Liu S AD - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China; MOE Innovation Center for Basic Research in Tumor Immunotherapy, Hefei, China; Anhui Province Key Laboratory of Tumor Immune Microenvironment and Immunotherapy, Hefei, China. FAU - Gu, Jinghua AU - Gu J AD - First Clinical Medical College (First Affiliated Hospital), Anhui Medical University, Hefei, China; Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China. FAU - Sheng, Shuyan AU - Sheng S AD - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China; MOE Innovation Center for Basic Research in Tumor Immunotherapy, Hefei, China; Anhui Province Key Laboratory of Tumor Immune Microenvironment and Immunotherapy, Hefei, China. FAU - Zhang, Shaobo AU - Zhang S AD - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China; MOE Innovation Center for Basic Research in Tumor Immunotherapy, Hefei, China; Anhui Province Key Laboratory of Tumor Immune Microenvironment and Immunotherapy, Hefei, China. FAU - Fei, Zimao AU - Fei Z AD - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China; MOE Innovation Center for Basic Research in Tumor Immunotherapy, Hefei, China; Anhui Province Key Laboratory of Tumor Immune Microenvironment and Immunotherapy, Hefei, China. FAU - Li, Conghan AU - Li C AD - Department of General Surgery, The Second Affiliated Hospital of Anhui Medical University, Hefei, China. FAU - Hu, Kaipeng AU - Hu K AD - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China; MOE Innovation Center for Basic Research in Tumor Immunotherapy, Hefei, China; Anhui Province Key Laboratory of Tumor Immune Microenvironment and Immunotherapy, Hefei, China. FAU - Jiang, Chenyao AU - Jiang C AD - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China; MOE Innovation Center for Basic Research in Tumor Immunotherapy, Hefei, China; Anhui Province Key Laboratory of Tumor Immune Microenvironment and Immunotherapy, Hefei, China. FAU - Bu, Fangzhao AU - Bu F AD - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China; MOE Innovation Center for Basic Research in Tumor Immunotherapy, Hefei, China; Anhui Province Key Laboratory of Tumor Immune Microenvironment and Immunotherapy, Hefei, China. FAU - Qin, Xihu AU - Qin X AD - Department of Hepato-biliary-pancreatic Surgery, the Third Affiliated Hospital of Nanjing Medical University, Changzhou, China. FAU - Xu, Qingxiang AU - Xu Q AD - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China; MOE Innovation Center for Basic Research in Tumor Immunotherapy, Hefei, China; Anhui Province Key Laboratory of Tumor Immune Microenvironment and Immunotherapy, Hefei, China. Electronic address: xqx@ahmu.edu.cn. FAU - Xu, Zhu AU - Xu Z AD - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China; MOE Innovation Center for Basic Research in Tumor Immunotherapy, Hefei, China; Anhui Province Key Laboratory of Tumor Immune Microenvironment and Immunotherapy, Hefei, China. Electronic address: xuzhu@ahmu.edu.cn. FAU - Sun, Beicheng AU - Sun B AD - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China; MOE Innovation Center for Basic Research in Tumor Immunotherapy, Hefei, China; Anhui Province Key Laboratory of Tumor Immune Microenvironment and Immunotherapy, Hefei, China. Electronic address: sunbc@ahmu.edu.cn. FAU - Xia, Anliang AU - Xia A AD - Department of Hepatobiliary Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China; MOE Innovation Center for Basic Research in Tumor Immunotherapy, Hefei, China; Anhui Province Key Laboratory of Tumor Immune Microenvironment and Immunotherapy, Hefei, China. Electronic address: xiaanliang@ahmu.edu.cn. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - JHEP Rep JT - JHEP reports : innovation in hepatology JID - 101761237 SB - IM OTO - NOTNLM OT - Cell-cell communication OT - Extracellular matrix OT - Fibroblast heterogeneity OT - Immunosuppressive niche OT - Myeloid biology COIS- Conflict of interest statement The authors declare no competing interests. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:33 PHST- 2025/11/06 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:33 [entrez] AID - S2589-5559(26)00356-3 [pii] AID - 10.1016/j.jhepr.2026.102085 [doi] PST - aheadofprint SO - JHEP Rep. 2026 Oct 28:102085. doi: 10.1016/j.jhepr.2026.102085. PMID- 42951071 OWN - NLM STAT- Publisher LR - 20261028 IS - 1878-8769 (Electronic) IS - 1878-8750 (Linking) DP - 2026 Oct 28 TI - Development and Evaluation of a Dual-Articulating Rigid Endoscope for Neuroendoscopic Surgery. PG - 125457 LID - S1878-8750(26)00673-X [pii] LID - 10.1016/j.wneu.2026.125457 [doi] AB - BACKGROUND: Neuroendoscopic surgery offers minimally invasive access with enhanced visualization; however, limited maneuverability within narrow corridors often leads to interference between the endoscope shaft and surgical instruments. We developed a dual-articulating rigid endoscope (DARE) to address this limitation. METHODS: A prototype DARE (5.4 mm diameter, 200 mm length) with two articulating segments underwent quantitative field-of-view assessment, neuroendoscopic surgery model testing and cadaveric evaluation. Field of view, surgical performance and instrument interference were compared with those of conventional rigid endoscopes. Cadaveric procedures included optic canal decompression via an endonasal paraseptal transsphenoidal approach and pineal region dissection via an endoscopic occipital transtentorial approach. RESULTS: The DARE provided a wider horizontal angular field and a larger maximum observable angle than conventional rigid endoscopes. In the surgical model, the DARE provided a sufficient field of view at both central and peripheral regions of the model while minimizing interference with instruments in both hands, even when positioned close to the target. In contrast, conventional rigid endoscopes required oblique viewing to visualize the periphery and showed increased interference during close-up manipulation. In cadaveric dissections, the device improved the freedom of bimanual instrument handling in anatomically complex regions where interference is typically problematic. However, operation of the endoscope required an additional operator, and a second endoscope was sometimes necessary for safe monitoring. CONCLUSIONS: The DARE provided a large maximum observable angle and effectively reduced instrument interference and expanded the operative workspace. Although further refinement is needed, this device has the potential to advance neuroendoscopic surgery. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Inc. All rights reserved. FAU - Fujinami, Ryota AU - Fujinami R AD - Department of Neurosurgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. Electronic address: ryotafujinami@gmail.com. FAU - Yamada, Hiroshi AU - Yamada H AD - Department of Neurosurgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. Electronic address: hyamada@med.nagoya-cu.ac.jp. FAU - Nozaki, Youshirou AU - Nozaki Y AD - Department of Neurosurgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. Electronic address: soflno8ctb@gmail.com. FAU - Yamada, Shigeki AU - Yamada S AD - Department of Neurosurgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. Electronic address: shigekiyamada393@gmail.com. FAU - Ueki, Takatoshi AU - Ueki T AD - Department of Integrative Anatomy, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. Electronic address: ueki@med.nagoya-cu.ac.jp. FAU - Tanikawa, Motoki AU - Tanikawa M AD - Department of Neurosurgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. Electronic address: mtnkw@med.nagoya-cu.ac.jp. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - World Neurosurg JT - World neurosurgery JID - 101528275 SB - IM OTO - NOTNLM OT - cadaveric study OT - dual-articulating endoscope OT - instrument interference OT - neuroendoscopic surgery OT - surgical simulation model EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:33 PHST- 2026/05/18 00:00 [received] PHST- 2026/10/20 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:33 [entrez] AID - S1878-8750(26)00673-X [pii] AID - 10.1016/j.wneu.2026.125457 [doi] PST - aheadofprint SO - World Neurosurg. 2026 Oct 28:125457. doi: 10.1016/j.wneu.2026.125457. PMID- 42951070 OWN - NLM STAT- Publisher LR - 20261028 IS - 1878-8769 (Electronic) IS - 1878-8750 (Linking) DP - 2026 Oct 28 TI - Who Benefits From Aggressive Surgery? Predictors of Survival and Short-Term Functional Status After Decompressive Craniectomy for Penetrating Traumatic Brain Injury in the Gaza War: A Consecutive Wartime Cohort. PG - 125461 LID - S1878-8750(26)00677-7 [pii] LID - 10.1016/j.wneu.2026.125461 [doi] AB - OBJECTIVE: Operative series of penetrating traumatic brain injury (pTBI) are vulnerable to treatment-selection bias because patients with adverse features are often managed non-operatively. We evaluated all-cause in-hospital mortality and short-term discharge function in a consecutive wartime cohort under a permissive decompressive craniectomy policy. METHODS: We retrospectively analyzed a two-center 2025 registry including 151 consecutive patients with CT-confirmed pTBI who underwent decompressive craniectomy. The primary outcome was all-cause in-hospital mortality; the secondary outcome was discharge Glasgow Outcome Scale (GOS) among survivors with internally consistent entries. Mortality associations were assessed using Fisher exact tests and a 4-covariate Firth penalized logistic model with bootstrap optimism correction and post-hoc intercept recalibration. RESULTS: Mortality was 67/147 (46%; 95% CI 37.4-54.0); assigning the 4 unresolved cases as survivors or deaths yielded a whole-cohort range of 44%-47%. Bilaterally fixed and dilated pupils were associated with 10/12 deaths (83%) and remained the only independent predictor in the prespecified model (OR 9.38, 95% CI 1.48-59.4). Transventricular injury carried 13/20 mortality (65%), whereas bihemispheric trajectory was not associated with death. Among 80 survivors, 60 had internally consistent discharge GOS and 41/60 (68%) had GOS 4-5. Three-month GOS was available in only 12/151 patients. CONCLUSIONS: Severe trajectory features were not uniformly fatal, whereas bilaterally fixed and dilated pupils remained the strongest independent prognostic marker. The findings argue against absolute futility judgments based on trajectory alone. Because discharge function was incompletely ascertained and longer-term follow-up was limited, these data demonstrate survivability under a permissive wartime operative policy rather than long-term functional benefit. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Alshawwaf, Furat K M AU - Alshawwaf FKM AD - Department of Neurosurgery, Nasser Medical Complex (NMC), Ministry of Health, Khan Younis, Gaza, Palestine. Electronic address: q40668881@gmail.com. FAU - Ayyad, Ali AU - Ayyad A AD - Department of Neurosurgery, Jordan University Hospital, Amman, Jordan; Department of Neurosurgery, Faculty of Medicine, The University of Jordan, Amman, Jordan. Electronic address: aayyad@web.de. FAU - Abu Alia, Luay A AU - Abu Alia LA AD - The Hashemite University, Amman, Jordan. Electronic address: luaya@hu.edu.jo. FAU - Alaqad, Alaa AU - Alaqad A AD - Department of Neurosurgery, Nasser Medical Complex (NMC), Ministry of Health, Khan Younis, Gaza, Palestine; Department of Neurosurgery, European Gaza Hospital (EGH), Ministry of Health, Khan Younis, Gaza, Palestine. Electronic address: alaa1931997@gmail.com. FAU - Abu-Qadous, Fatma F AU - Abu-Qadous FF AD - Department of Neurosurgery, Nasser Medical Complex (NMC), Ministry of Health, Khan Younis, Gaza, Palestine. Electronic address: Falhoobi@gmail.com. FAU - Tabash, Mohammed Y AU - Tabash MY AD - Department of Neurosurgery, Nasser Medical Complex (NMC), Ministry of Health, Khan Younis, Gaza, Palestine. Electronic address: mohammed.tabash25@gmail.com. FAU - Alhussein, Nouraldeen Abu AU - Alhussein NA AD - Department of Neurosurgery, Nasser Medical Complex (NMC), Ministry of Health, Khan Younis, Gaza, Palestine. Electronic address: nourdeen044@gmail.com. FAU - Alqatati, Mohammed F AU - Alqatati MF AD - Department of Neurosurgery, Nasser Medical Complex (NMC), Ministry of Health, Khan Younis, Gaza, Palestine. Electronic address: mohdfadel1993@gmail.com. FAU - Barhoom, Raed AU - Barhoom R AD - Department of Neurosurgery, Nasser Medical Complex (NMC), Ministry of Health, Khan Younis, Gaza, Palestine. Electronic address: raednasralla94@gmail.com. FAU - Alhoobi, Mohammed M M AU - Alhoobi MMM AD - Department of Neurosurgery, Nasser Medical Complex (NMC), Ministry of Health, Khan Younis, Gaza, Palestine; Department of Neurosurgery, European Gaza Hospital (EGH), Ministry of Health, Khan Younis, Gaza, Palestine. Electronic address: Malhoobi@gmail.com. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - World Neurosurg JT - World neurosurgery JID - 101528275 SB - IM OTO - NOTNLM OT - Decompressive craniectomy OT - Gaza OT - Glasgow Outcome Scale OT - Global neurosurgery OT - Mass casualty OT - Penetrating traumatic brain injury OT - Triage EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:33 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/20 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:33 [entrez] AID - S1878-8750(26)00677-7 [pii] AID - 10.1016/j.wneu.2026.125461 [doi] PST - aheadofprint SO - World Neurosurg. 2026 Oct 28:125461. doi: 10.1016/j.wneu.2026.125461. PMID- 42951067 OWN - NLM STAT- Publisher LR - 20261028 IS - 1878-8769 (Electronic) IS - 1878-8750 (Linking) DP - 2026 Oct 28 TI - Laboratory Parameters Associated with Reoperation in Chronic Subdural Hematoma: A Retrospective Cohort Study with Sex-Stratified Analyses. PG - 125455 LID - S1878-8750(26)00671-6 [pii] LID - 10.1016/j.wneu.2026.125455 [doi] AB - BACKGROUND: Chronic subdural hematoma (cSDH) frequently recurs after surgery, yet the prognostic relevance of routinely available laboratory parameters remains insufficiently characterized. This study evaluated demographic and laboratory correlates of reoperation after cSDH surgery, with emphasis on sex-specific differences. METHODS: We retrospectively analyzed 310 consecutive adults undergoing cSDH surgery at a tertiary center (2015-2024). Demographic and admission laboratory variables were assessed using univariable comparisons with Benjamini-Hochberg correction, followed by multivariable logistic and LASSO regression with prespecified candidate variables, including exploratory sex-stratified analyses. RESULTS: Seventy-seven patients (25%) underwent reoperation, with a median interval of 15 days. Median age was 77 years, and 70% were male. In multivariable analysis, lower platelet count was associated with reoperation in the overall cohort (OR 0.99, p = 0.021). In the sex-stratified multivariable analysis, lower body weight (OR 0.96, p = 0.033), lower platelet count (OR 0.99, p = 0.005), and higher TSH (OR 1.60, p = 0.004) were independently associated with reoperation among male patients. The female-specific LASSO model did not identify any variables with reoperation. However, the limited number of reoperation events (n = 18) in this subgroup and poor cross-validated model discrimination (AUC 0.43) substantially constrain the interpretation of this result. CONCLUSIONS: Routinely available baseline laboratory parameters, particularly lower platelet count, may provide relevant prognostic information for reoperation after cSDH surgery. Sex-stratified analyses identified additional but exploratory associations, suggesting that sex may modestly influence the contribution of individual laboratory parameters to reoperation risk. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Inc. All rights reserved. FAU - Schlicht, Frederick AU - Schlicht F AD - Department of Neurosurgery, Jena University Hospital, Friedrich-Schiller-University Jena, Am Klinikum 1, 07747 Jena, Germany. Electronic address: frederick.schlicht@uni-jena.de. FAU - Aboud, Nazeer AU - Aboud N AD - Department of Neurosurgery, Jena University Hospital, Friedrich-Schiller-University Jena, Am Klinikum 1, 07747 Jena, Germany. FAU - Borda, Ricardo AU - Borda R AD - Department of Statistics, El Bosque University, 110121 Bogota, Colombia. FAU - Sarmiento, Daniel AU - Sarmiento D AD - Department of Statistics, El Bosque University, 110121 Bogota, Colombia. FAU - Senft, Christian AU - Senft C AD - Department of Neurosurgery, Jena University Hospital, Friedrich-Schiller-University Jena, Am Klinikum 1, 07747 Jena, Germany. FAU - Dinc, Nazife AU - Dinc N AD - Department of Neurosurgery, Jena University Hospital, Friedrich-Schiller-University Jena, Am Klinikum 1, 07747 Jena, Germany. FAU - Schwarz, Falko AU - Schwarz F AD - Department of Neurosurgery, Jena University Hospital, Friedrich-Schiller-University Jena, Am Klinikum 1, 07747 Jena, Germany. FAU - Calero-Martinez, Sergio A AU - Calero-Martinez SA AD - Department of Neurosurgery, Jena University Hospital, Friedrich-Schiller-University Jena, Am Klinikum 1, 07747 Jena, Germany; Instituto de Neurociencias, Faculty of Medicine, El Bosque University, 110121 Bogota, Colombia. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - World Neurosurg JT - World neurosurgery JID - 101528275 SB - IM OTO - NOTNLM OT - Least Absolute Shrinkage and Selection Operator OT - chronic subdural hematoma OT - laboratory biomarkers OT - reoperation OT - sex-specific EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:33 PHST- 2026/06/04 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:33 [entrez] AID - S1878-8750(26)00671-6 [pii] AID - 10.1016/j.wneu.2026.125455 [doi] PST - aheadofprint SO - World Neurosurg. 2026 Oct 28:125455. doi: 10.1016/j.wneu.2026.125455. PMID- 42951050 OWN - NLM STAT- Publisher LR - 20261028 IS - 1097-6809 (Electronic) IS - 0741-5214 (Linking) DP - 2026 Oct 28 TI - Carotid Endarterectomy Versus Trans-Carotid Artery Revascularisation (TCAR) for Asymptomatic Carotid Stenosis A Systematic Review and Meta-Analysis. LID - S0741-5214(26)01807-0 [pii] LID - 10.1016/j.jvs.2026.10.052 [doi] AB - OBJECTIVES: The treatment for asymptomatic carotid artery stenosis includes a surgical or interventional approach in selected cases. Although endarterectomy should be considered over stenting for selected patients, according to the latest European Society of Vascular Surgery (ESVS) guidelines, the Carotid Revascularization and Medical Management for Asymptomatic Carotid Stenosis Trials (CREST-2) suggested that carotid stenting would be associated with low rates of perioperative stroke or death. The transcarotid artery revascularization (TCAR) technique is a relatively new endovascular method, which involves placing a stent at the carotid bifurcation through direct carotid artery access, performed under a flow reversal system. This approach avoids manipulation of the aortic arch, potentially reducing embolic events. The aim of this systematic review is to compare TCAR with carotid endarterectomy (CEA) in asymptomatic patients undergoing carotid intervention. It seeks to evaluate whether TCAR is associated with improved perioperative outcomes, such as lower rates of stroke and death, compared with CEA, given its potentially less invasive approach. METHODS: The review was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) on the platforms PubMed/Medline, OVID/Embase, and Scopus. The following MeSH terms were used: "asymptomatic carotid stenosis" AND "carotid endarterectomy", "transcarotid artery revascularization" OR "TCAR". Full-text screening and data extraction were performed with the support of the COVIDENCE platform. Six studies were selected for this systematic review, comprising a total of 132,684 patients (30,581 undergoing TCAR and 102,103 undergoing CEA). Statistical analysis was performed using R software. RESULTS: The selected studies for this systematic review showed similar results in terms of perioperative stroke and death for asymptomatic patients who underwent carotid endarterectomy and TCAR. CONCLUSION: Our results suggest that there are no significant differences between CEA and TCAR for the treatment of asymptomatic patients who require a surgical or interventional approach. Further studies are necessary to provide more robust data in this field. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Domingos Rosa Schneidwind, Karina AU - Domingos Rosa Schneidwind K AD - University of Freiburg. Electronic address: karinarosa@me.com. FAU - Mofidi, Reza AU - Mofidi R AD - University of Edinburgh. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - J Vasc Surg JT - Journal of vascular surgery JID - 8407742 SB - IM OTO - NOTNLM OT - "Asymptomatic Carotid Stenosis" OT - "CEA" OT - "Carotid Endarterectomy" OT - "TCAR" OT - "Trans Carotid Artey Stenosis" EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:33 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:33 [entrez] AID - S0741-5214(26)01807-0 [pii] AID - 10.1016/j.jvs.2026.10.052 [doi] PST - aheadofprint SO - J Vasc Surg. 2026 Oct 28:S0741-5214(26)01807-0. doi: 10.1016/j.jvs.2026.10.052. PMID- 42951048 OWN - NLM STAT- Publisher LR - 20261028 IS - 1097-6809 (Electronic) IS - 0741-5214 (Linking) DP - 2026 Oct 28 TI - Ultrasound-guided selective nerve block promotes perioperative vascular remodeling and early maturation of radiocephalic arteriovenous fistulas. LID - S0741-5214(26)01774-X [pii] LID - 10.1016/j.jvs.2026.10.020 [doi] AB - BACKGROUND: Autogenous arteriovenous fistula (AVF) is the preferred vascular access for maintenance hemodialysis, but its successful maturation depends on adequate perioperative vascular remodeling. We evaluated whether ultrasound-guided selective nerve block (SNB) promotes vascular remodeling and improves AVF maturation after radiocephalic AVF creation. METHODS: This was a retrospective analysis based on a prospectively-maintained single-center vascular-access clinical follow-up database. We included 163 patients with stage 5 chronic kidney disease undergoing primary radiocephalic AVF creation between January 2023 and January 2024. Patients received ultrasound-guided selective nerve block (SNB, n=72) or local infiltration anesthesia (LA, n=91). Perioperative changes in radial artery and cephalic vein diameters were evaluated, postoperative brachial artery blood flow was recorded, and AVF maturation was assessed at 1, 2, and 3 months. Linear mixed-effects models and multivariable logistic regression were used for analysis. RESULTS: Compared with LA, SNB significantly increased radial artery and cephalic vein diameters after anesthesia, whereas both vessels constricted in the LA group (both P<0.001). Linear mixed-effects analysis demonstrated significant time effects and group-by-time interactions for both vessels, while a significant group effect was observed only for the cephalic vein (all P<0.001). Postoperative brachial artery blood flow was significantly greater in the SNB group (P<0.001). The 2-month AVF maturation rate was significantly higher in the SNB group than in the LA group (60% vs. 35%, P=0.002), whereas no between-group differences were observed at 1 or 3 months. Younger age, larger preoperative radial artery diameter, and SNB were independent predictors of AVF maturation. CONCLUSIONS: Ultrasound-guided selective nerve block promotes perioperative vascular remodeling, increases postoperative blood flow, and independently facilitates early AVF maturation after radiocephalic AVF creation. These findings support SNB as a promising perioperative strategy to optimize vascular access outcomes in patients undergoing AVF creation. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Wen, Jing AU - Wen J AD - Department of Nephrology, Haidian Hospital (Haidian section of Peking University Third Hospital), Beijing, China. FAU - Zhan, Shen AU - Zhan S AD - Department of Nephrology, Haidian Hospital (Haidian section of Peking University Third Hospital), Beijing, China. FAU - Zhang, Lihong AU - Zhang L AD - Department of Nephrology, Haidian Hospital (Haidian section of Peking University Third Hospital), Beijing, China. FAU - Wang, Yuzhu AU - Wang Y AD - Department of Nephrology, Haidian Hospital (Haidian section of Peking University Third Hospital), Beijing, China. Electronic address: wangyuzhu_13579@sina.com. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Vasc Surg JT - Journal of vascular surgery JID - 8407742 SB - IM OTO - NOTNLM OT - Chronic kidney disease OT - arteriovenous fistula maturation OT - hemodialysis OT - selective nerve block OT - vascular remodeling EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:33 PHST- 2026/08/05 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:33 [entrez] AID - S0741-5214(26)01774-X [pii] AID - 10.1016/j.jvs.2026.10.020 [doi] PST - aheadofprint SO - J Vasc Surg. 2026 Oct 28:S0741-5214(26)01774-X. doi: 10.1016/j.jvs.2026.10.020. PMID- 42951047 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-260X (Electronic) IS - 0925-4439 (Linking) DP - 2026 Oct 28 TI - Targeting triple-negative breast cancer: Molecular basis and emerging antibody-drug conjugate strategies. PG - 168515 LID - S0925-4439(26)00381-9 [pii] LID - 10.1016/j.bbadis.2026.168515 [doi] AB - Triple-negative breast cancer (TNBC) accounts for ~10-15% of all breast cancers and is an aggressive, molecularly heterogeneous subtype associated with poor clinical outcomes and limited targeted treatment options. Consequently, systemic therapy has historically relied on chemotherapy, which is often compromised by recurrence, dose-limiting toxicity, and the rapid development of therapeutic resistance. Antibody-drug conjugates (ADCs) have emerged as a transformative therapeutic platform by combining tumor-specific antibody targeting highly potent cytotoxic payloads through advances in antibody engineering, linker chemistry, and payload design. This mini-review summarizes recent advances in ADC development for TNBC, with a particular focus on the molecular basis of ADC therapy, the chemical determinants governing efficacy and selectivity, emerging mechanisms of resistance, and next-generation strategies to improve therapeutic durability and clinical outcomes. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Feng, Haosheng AU - Feng H AD - Center for Nuclear Receptors and Cell Signaling, University of Houston, Houston, TX, 77204, United States; Department of Biology and Biochemistry, University of Houston, Houston, TX, 77204, United States. FAU - Padala, Chiranjeevi AU - Padala C AD - Center for Nuclear Receptors and Cell Signaling, University of Houston, Houston, TX, 77204, United States; Department of Biology and Biochemistry, University of Houston, Houston, TX, 77204, United States. FAU - Liu, Bin AU - Liu B AD - Center for Nuclear Receptors and Cell Signaling, University of Houston, Houston, TX, 77204, United States; Department of Biology and Biochemistry, University of Houston, Houston, TX, 77204, United States; Department of Chemistry, University of Houston, Houston, TX, 77204, United States; Department of Biomedical Engineering, University of Houston, Houston, TX, 77204, United States; Program of Materials Science and Engineering, University of Houston, Houston, TX, 77204, United States. Electronic address: bliu39@central.uh.edu. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - Netherlands TA - Biochim Biophys Acta Mol Basis Dis JT - Biochimica et biophysica acta. Molecular basis of disease JID - 101731730 SB - IM OTO - NOTNLM OT - Antibody-drug conjugates OT - Disease molecular mechanism OT - Drug resistance OT - Targeted therapy OT - Triple-negative breast cancer COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:33 PHST- 2026/05/01 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:33 [entrez] AID - S0925-4439(26)00381-9 [pii] AID - 10.1016/j.bbadis.2026.168515 [doi] PST - aheadofprint SO - Biochim Biophys Acta Mol Basis Dis. 2026 Oct 28:168515. doi: 10.1016/j.bbadis.2026.168515. PMID- 42951044 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-260X (Electronic) IS - 0925-4439 (Linking) DP - 2026 Oct 28 TI - Beyond pyroptosis: Non-lytic functions of GSDMD in tumor immunity and cancer immunotherapy. PG - 168519 LID - S0925-4439(26)00385-6 [pii] LID - 10.1016/j.bbadis.2026.168519 [doi] AB - Gasdermin D (GSDMD) is classically defined as the pore-forming executioner of pyroptosis, but GSDMD activation does not invariably culminate in terminal lysis. Direct mechanistic studies show that limited GSDMD pores can support interleukin-1beta (IL-1beta), ATP, or interferon-stimulated gene 15 (ISG15) release before overt plasma-membrane rupture, whereas Ca(2+)-triggered endosomal sorting complex required for transport (ESCRT)-III recruitment and ALG-2-interacting protein X (ALIX)-dependent endosomal clearance can limit or reverse membrane injury. Nerve injury-induced protein 1 (NINJ1) acts downstream of early pore-mediated permeability to execute terminal plasma-membrane rupture and macromolecular damage-associated molecular pattern (DAMP) release. Emerging evidence extends GSDMD activity to nuclear-envelope transport and extracellular-vesicle-mediated transfer of pore structures, although these mechanisms are model-specific and are not uniformly non-lytic. In tumor immunity, GSDMD has opposing compartment-dependent effects. In a B16F10 anti-programmed death-ligand 1 (PD-L1) model, GSDMD in Cx3cr1(+) myeloid/antigen-presenting cell (APC) populations restricted a cyclic GMP-AMP synthase (cGAS)-dependent ISG program. In MC38 and KPC models, non-lytic GSDMD pores in CD4(+) T cells amplified Ca(2+)-nuclear factor of activated T cells (NFAT)-IL-2 helper signaling. In selected colorectal cancer (CRC) and oral squamous cell carcinoma (OSCC) models, distinct full-length, nuclear, or cleavage-associated GSDMD states regulated DNA repair, growth, metastasis, and immune exclusion. In selected pancreatic and ovarian cancer models, treatment-induced gasdermin activation contributed to immunogenic lytic death. This review distinguishes established basic mechanisms, model-defined causal evidence, retrospective associations, and hypothesis-generating translation. We propose cell-type- and state-resolved biomarkers and compartment-selective interventions as prospective research frameworks rather than clinically validated decision tools. Standardized functional assays, unified multicompartment models, selective delivery, and prospective human validation are required before GSDMD can be considered clinically actionable. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Wang, Xiaoying AU - Wang X AD - Wuxi School of Medicine, Jiangnan University, Wuxi, 214122, China; Medical Basic Research Innovation Center for Gut Microbiota and Chronic Diseases, Wuxi School of Medicine, Jiangnan University, Wuxi, 214122, China; Public Health Research Center and Department of Public Health and Preventive Medicine, Wuxi School of Medicine, Jiangnan University, Wuxi, Jiangsu, China. Electronic address: xiaoyingwang@jiangnan.edu.cn. FAU - Du, Qindan AU - Du Q AD - Wuxi School of Medicine, Jiangnan University, Wuxi, 214122, China. FAU - Wu, Junjie AU - Wu J AD - Institute of Integrated Chinese and Western Medicine, Affiliated Hospital of Jiangnan University, Wuxi, China. FAU - Han, Zhen AU - Han Z AD - Wuxi School of Medicine, Jiangnan University, Wuxi, 214122, China. FAU - Zhang, Yumeng AU - Zhang Y AD - Wuxi School of Medicine, Jiangnan University, Wuxi, 214122, China. FAU - Hu, Xuewen AU - Hu X AD - Wuxi School of Medicine, Jiangnan University, Wuxi, 214122, China. FAU - Xiong, Rui AU - Xiong R AD - Wuxi School of Medicine, Jiangnan University, Wuxi, 214122, China. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - Netherlands TA - Biochim Biophys Acta Mol Basis Dis JT - Biochimica et biophysica acta. Molecular basis of disease JID - 101731730 SB - IM OTO - NOTNLM OT - Candidate biomarkers OT - Cell-type specificity OT - GSDMD OT - Immune checkpoint blockade OT - Membrane repair OT - NINJ1 OT - Non-pyroptotic signaling OT - Sublytic pores OT - Tumor immunity OT - Viable-cell secretion COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:33 PHST- 2026/06/30 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:33 [entrez] AID - S0925-4439(26)00385-6 [pii] AID - 10.1016/j.bbadis.2026.168519 [doi] PST - aheadofprint SO - Biochim Biophys Acta Mol Basis Dis. 2026 Oct 28:168519. doi: 10.1016/j.bbadis.2026.168519. PMID- 42951041 OWN - NLM STAT- Publisher LR - 20261028 IS - 1096-0333 (Electronic) IS - 0041-008X (Linking) DP - 2026 Oct 28 TI - Naringin attenuates melamine-induced renal biochemical alterations in rats: Histopathological and immunohistochemical assessment. PG - 118095 LID - S0041-008X(26)00391-1 [pii] LID - 10.1016/j.taap.2026.118095 [doi] AB - BACKGROUND: Naringin has shown nephroprotective activity in several toxicant models. This study evaluated renal outcomes during concurrent naringin administration and repeated melamine exposure. METHODS: Thirty Sprague-Dawley rats were allocated to five groups, each containing three males and three females: control, naringin alone (100 mg/kg/day), melamine alone (300 mg/kg/day), and melamine plus naringin (50 or 100 mg/kg/day). Treatment continued orally for 28 days. We assessed serum creatinine, urea, and uric acid; renal superoxide dismutase activity; tumor necrosis factor-alpha concentrations; histopathology; and 15A3 immunoreactivity. Factorial models used individual SOD values and rounded group means, standard deviations, and sample sizes for the other biochemical outcomes to estimate treatment contrasts averaged equally across sexes. Tissue-score frequencies were summarized descriptively. RESULTS: Treatment-group effects were significant for all five biochemical outcomes (all p < 0.001). Compared with melamine alone, the 100 mg/kg naringin regimen was associated with a sex-balanced mean creatinine difference of -0.640 mg/dL (Tukey-adjusted 95% CI -0.809 to -0.471). Both naringin regimens showed partial attenuation of the measured biochemical abnormalities, with larger differences at 100 mg/kg. These contrasts remained significant in a heteroscedastic sensitivity analysis. Renal SOD activity was higher than with melamine alone but remained below control values. Selected within-sex pairwise comparisons of H&E injury scores and 15A3 staining categories among the melamine-only and two co-treatment groups were not significant after Holm adjustment (all adjusted p >/= 0.300). Separate weekly treatment-group comparisons of body weight were not significant. CONCLUSIONS: Concurrent naringin administration was associated with partial attenuation of the measured biochemical abnormalities during melamine exposure. The selected pairwise analyses did not establish differences in H&E injury or 15A3 staining categories among the melamine-only and co-treatment groups. These findings do not establish recovery of renal filtration or tubular function. Small samples, summary-data inference, and antibody specificity limit conclusions about sex differences and DNA-specific injury. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Hemeda, Mohamed S AU - Hemeda MS AD - Department of Forensic Medicine and Clinical Toxicology, Port Said University, Port Said, Egypt. Electronic address: mohamudsadawy@med.psu.edu.eg. FAU - Farrag, Mayada Saad AU - Farrag MS AD - Department of Pathology, Faculty of Medicine, Port Said University, Port Said, Egypt. FAU - Abdelkarim, Nourhan Mohamed AU - Abdelkarim NM AD - Department of Human Anatomy and Embryology, Faculty of Medicine, Port Said University, Port Said, Egypt. FAU - Mohamed, Abdulrhman Al-Shahhat AU - Mohamed AA AD - Department of Human Anatomy and Embryology, Faculty of Medicine, Port Said University, Port Said, Egypt. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Toxicol Appl Pharmacol JT - Toxicology and applied pharmacology JID - 0416575 SB - IM OTO - NOTNLM OT - Flavanone glycoside OT - Oxidized guanosine OT - Superoxide dismutase OT - Tubular injury OT - Tumor necrosis factor alpha COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:32 PHST- 2026/10/04 00:00 [received] PHST- 2026/10/24 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:32 [entrez] AID - S0041-008X(26)00391-1 [pii] AID - 10.1016/j.taap.2026.118095 [doi] PST - aheadofprint SO - Toxicol Appl Pharmacol. 2026 Oct 28:118095. doi: 10.1016/j.taap.2026.118095. PMID- 42951037 OWN - NLM STAT- Publisher LR - 20261028 IS - 1573-2517 (Electronic) IS - 0165-0327 (Linking) DP - 2026 Oct 28 TI - Speech-based differentiation of unipolar and bipolar depression across scripted reading and interview questions. PG - 122675 LID - S0165-0327(26)01529-6 [pii] LID - 10.1016/j.jad.2026.122675 [doi] AB - BACKGROUND: Differentiating bipolar depression (BPD) from unipolar depression (UPD) remains clinically challenging. We examined whether acoustic and linguistic speech features could support this distinction and whether their informativeness varied across scripted reading and interview questions. METHODS: This study included 109 healthy controls (HCs), 52 patients with UPD, and 53 patients with BPD experiencing depressive episodes. Participants completed a scripted reading task and an interview-based speech task comprising 16 questions. Acoustic features were extracted from all audio recordings, and linguistic features were derived from interview transcripts. Group differences were examined using robust analysis of covariance with global false discovery rate correction. Two-level stacking models with fold-wise sex adjustment of acoustic features evaluated classification performance and importance of speech features and interview-based speech elicitation prompts. RESULTS: Multiple acoustic and linguistic features showed significant group differences in interview responses, whereas no acoustic features differed significantly during scripted reading. The best-performing models achieved F1 scores of 0.6655 for three-class classification (HC, UPD and BPD) and 0.6430 for binary classification (UPD and BPD). Within interview-based speech, statistical and machine learning analyses identified Average Pause Duration, Phonation Ratio, and quantity-related expressions as particularly informative features. Two interview questions about (hypo-)manic experiences contributed most to differentiating BPD from UPD. CONCLUSIONS: Acoustic and linguistic speech features may help differentiate BPD from UPD during depressive episodes, with their informativeness varying across speech tasks and interview questions. Our findings suggest that targeted interview-based speech assessment may have potential as a non-invasive adjunct to conventional clinical evaluation. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Zhong, Rongrong AU - Zhong R AD - Clinical Research Center & Division of Mood Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Biostatistics & Health Informatics, Institute of Psychiatry, Psychology and Neuroscience, King's College London, United Kingdom; College of Psychiatry and Clinical Psychology, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Zhang, Yuezhou AU - Zhang Y AD - Department of Biostatistics & Health Informatics, Institute of Psychiatry, Psychology and Neuroscience, King's College London, United Kingdom. FAU - Wu, Xiaohui AU - Wu X AD - Clinical Research Center & Division of Mood Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Cui, Lvchun AU - Cui L AD - Clinical Research Center & Division of Mood Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Lin, Jiansen AU - Lin J AD - Clinical Research Center & Division of Mood Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China; College of Psychiatry and Clinical Psychology, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Lv, Xue AU - Lv X AD - Clinical Research Center & Division of Mood Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Chen, Jiayue AU - Chen J AD - Clinical Research Center & Division of Mood Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Chen, Shen Tse AU - Chen ST AD - Clinical Research Center & Division of Mood Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Yu, Yue AU - Yu Y AD - Clinical Research Center & Division of Mood Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Folarin, Amos AU - Folarin A AD - Department of Biostatistics & Health Informatics, Institute of Psychiatry, Psychology and Neuroscience, King's College London, United Kingdom; Institute of Health Informatics, University College London, London, United Kingdom; NIHR Biomedical Research Centre at South London and Maudsley NHS Foundation Trust, London, United Kingdom; NIHR Biomedical Research Centre at University College London Hospitals NHS Foundation Trust, London, United Kingdom; Health Data Research UK, University College London, London, United Kingdom. FAU - Dobson, Richard AU - Dobson R AD - Department of Biostatistics & Health Informatics, Institute of Psychiatry, Psychology and Neuroscience, King's College London, United Kingdom; Institute of Health Informatics, University College London, London, United Kingdom; NIHR Biomedical Research Centre at South London and Maudsley NHS Foundation Trust, London, United Kingdom; NIHR Biomedical Research Centre at University College London Hospitals NHS Foundation Trust, London, United Kingdom; Health Data Research UK, University College London, London, United Kingdom. FAU - Chen, Jun AU - Chen J AD - Clinical Research Center & Division of Mood Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address: doctorcj2010@gmail.com. FAU - Fang, Yiru AU - Fang Y AD - College of Psychiatry and Clinical Psychology, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Department of Psychiatry & Affective Disorders Center, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Key Laboratory of Psychotic Disorders, Shanghai, China. Electronic address: yirufang@aliyun.com. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - J Affect Disord JT - Journal of affective disorders JID - 7906073 SB - IM OTO - NOTNLM OT - Bipolar depression OT - Diagnosis OT - Machine learning OT - Speech OT - Unipolar depression COIS- Declaration of competing interest Amos Folarin and Richard Dobson are co-founders of Onsentia. Amos Folarin holds shares of Google. Other authors have no conflicts of interest to declare that are relevant to the content of this article. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:32 PHST- 2026/07/30 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:32 [entrez] AID - S0165-0327(26)01529-6 [pii] AID - 10.1016/j.jad.2026.122675 [doi] PST - aheadofprint SO - J Affect Disord. 2026 Oct 28:122675. doi: 10.1016/j.jad.2026.122675. PMID- 42951035 OWN - NLM STAT- Publisher LR - 20261028 IS - 1874-1754 (Electronic) IS - 0167-5273 (Linking) DP - 2026 Oct 28 TI - Echocardiographic atrial and right heart to pulmonary circulation coupling indices identify high-risk in moderate aortic stenosis. PG - 135029 LID - S0167-5273(26)00879-X [pii] LID - 10.1016/j.ijcard.2026.135029 [doi] AB - PURPOSE: Emerging evidence points on an increased mortality risk associated to moderate aortic stenosis (AS), yet this population remains insufficiently characterized. We tested the prognostic value of two echocardiographic coupling indices reflecting right and left-sided cardiac function: the ratio of tricuspid annular plane systolic excursion to pulmonary artery systolic pressure ratio (TAPSE/PASP) and the left atrial volumetric/mechanical coupling index (LACI). METHODS: Retrospective, single-center study including 135 patients with moderate AS. TAPSE/PASP was used as a non-invasive surrogate of right ventricular-pulmonary circulation (RV-Pc) coupling, and LACI was defined as ratio of left atrial volume index to mitral a' velocity. The primary endpoint was all-cause mortality. Cox regression and Kaplan-Meier analyses were used to assess the prognostic value of both indices. RESULTS: During a mean follow-up of 1.61 +/- 0.79 years, 33 patients (24%) died. Reduced TAPSE/PASP (< 0.67 mm/mmHg) and elevated LACI (> 5.17) were independently associated with an increased mortality (HR: 3.03 and 3.16, respectively, p < 0.01). These associations remained significant even after adjusting for age. Stratification into three groups - preserved, single, or combined uncoupling -showed progressively lower 1-year survival rates, ranging from 95% to 49% (p < 0.0001). None of the coupling-derived variables was associated with hemodynamic progression or need of aortic valve replacement. CONCLUSION: TAPSE/PASP and LACI are independently associated with mortality in patients with moderate AS. These parameters may provide incremental prognostic information in this often-overlooked population. These findings may prompt further studies evaluating the potential role of these parameters in helping to refine prognostic phenotyping. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Santangelo, Gloria AU - Santangelo G AD - Department of Cardio-Thoracic-Vascular Diseases, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy. Electronic address: gloria.santangelo@policlinico.mi.it. FAU - Faggiano, Andrea AU - Faggiano A AD - Department of Cardio-Thoracic-Vascular Diseases, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy. FAU - Mallardi, Giulio Pio Federico AU - Mallardi GPF AD - Department of Cardio-Thoracic-Vascular Diseases, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy. FAU - Baroni, Michele AU - Baroni M AD - Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy. FAU - Donisi, Luca AU - Donisi L AD - Heart Rhythm Management Department, Clinique Pasteur, Toulouse, France. FAU - Guareschi, Alessandro AU - Guareschi A AD - Department of Cardio-Thoracic-Vascular Diseases, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy. FAU - Pasero, Guido AU - Pasero G AD - Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy. FAU - Sozzi, Fabiola AU - Sozzi F AD - Department of Cardio-Thoracic-Vascular Diseases, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy. FAU - Ruscica, Massimiliano AU - Ruscica M AD - Department of Cardio-Thoracic-Vascular Diseases, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; Department of Pharmacological and Biomolecular Sciences "Rodolfo Paoletti", University of Milan, Milan, Italy. Electronic address: massimiliano.ruscica@unimi.it. FAU - Guazzi, Marco AU - Guazzi M AD - University of Milan, Health Sciences Department, Milan, Italy; San Paolo University Hospital, Azienda Socio Sanitaria Territoriale Santi Paolo e Carlo, Milan, Italy. FAU - Carugo, Stefano AU - Carugo S AD - Department of Cardio-Thoracic-Vascular Diseases, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy; Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy. FAU - Bursi, Francesca AU - Bursi F AD - University of Milan, Health Sciences Department, Milan, Italy; San Paolo University Hospital, Azienda Socio Sanitaria Territoriale Santi Paolo e Carlo, Milan, Italy. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Int J Cardiol JT - International journal of cardiology JID - 8200291 SB - IM OTO - NOTNLM OT - LACI OT - Left atrioventricular coupling echocardiography OT - Moderate aortic stenosis OT - Right-heart coupling metric OT - TAPSE/PASP COIS- Declaration of competing interest No potential conflict of interest was reported by the authors. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:32 PHST- 2026/07/06 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:32 [entrez] AID - S0167-5273(26)00879-X [pii] AID - 10.1016/j.ijcard.2026.135029 [doi] PST - aheadofprint SO - Int J Cardiol. 2026 Oct 28:135029. doi: 10.1016/j.ijcard.2026.135029. PMID- 42951034 OWN - NLM STAT- Publisher LR - 20261028 IS - 1874-1754 (Electronic) IS - 0167-5273 (Linking) DP - 2026 Oct 28 TI - Coronary vascular dysfunction is associated with adverse left ventricular filling in patients with angina but no obstructed coronary arteries. PG - 135031 LID - S0167-5273(26)00881-8 [pii] LID - 10.1016/j.ijcard.2026.135031 [doi] AB - BACKGROUND: Angina with no obstructive coronary arteries is often due to coronary vascular dysfunction, which is increasingly described as a contributor and predictor of heart failure with preserved ejection fraction. We tested the hypothesis that coronary vascular dysfunction contributes to cardiac dysfunction. METHODS: Sixty-eight participants with suspected coronary vascular dysfunction underwent LV pressure-volume assessment at rest and during isometric handgrip stress. Coronary function testing was performed by infusing standard doses of adenosine, acetylcholine, and nitroglycerine through a guiding catheter placed in the left main coronary artery, per established protocols. RESULTS: Of the participants studied, 12 had normal coronary vascular function while 56 had coronary vascular dysfunction. At rest, there were no differences between groups in any invasive pressure or volumetric measurement. With isometric handgrip, peak filling rate increased significantly in participants with normal coronary vascular function (0.56 +/- 0.31 vs. 0.85 +/- 0.68 1/s/mmHg) but remained unchanged in participants with coronary vascular dysfunction (0.51 +/- 0.22 vs. 0.55 +/- 0.43 1/s/mmHg; group x time interaction, p = 0.04). Moreover, left ventricular end-diastolic pressure decreased significantly with isometric handgrip in participants with normal coronary vascular function (12 +/- 3 vs. 9 +/- 4 mmHg) but increased in participants with coronary vascular dysfunction (14 +/- 4 vs. 15 +/- 6 mmHg; group x time interaction, p = 0.03). CONCLUSIONS: Coronary vascular dysfunction was associated with an impaired ability of the LV to adapt its filling during stress. Further work is needed to assess potential clinical significance. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Oneglia, Andrew P AU - Oneglia AP AD - The University of Texas at Arlington, Department of Kinesiology, Arlington, TX, United States of America; CD Leycom, the Netherlands. FAU - Wei, Janet AU - Wei J AD - Cedars-Sinai Medical Center, Barbra Streisand Women's Heart Center, Smidt Heart Institute, Los Angeles, CA, United States of America. FAU - Samuels, Bruce AU - Samuels B AD - Cedars-Sinai Medical Center, Barbra Streisand Women's Heart Center, Smidt Heart Institute, Los Angeles, CA, United States of America. FAU - Samuel, T Jake AU - Samuel TJ AD - Johns Hopkins University School of Medicine, Department of Medicine, Baltimore, MD, United States of America. FAU - Sharif, Behzad AU - Sharif B AD - Cedars-Sinai Medical Center, Barbra Streisand Women's Heart Center, Smidt Heart Institute, Los Angeles, CA, United States of America; Weldon School of Biomedical Engineering, Purdue University, Indianapolis, IN, United States of America. FAU - Azarbal, Babak AU - Azarbal B AD - Cedars-Sinai Medical Center, Barbra Streisand Women's Heart Center, Smidt Heart Institute, Los Angeles, CA, United States of America. FAU - Petersen, John W AU - Petersen JW AD - University of Florida, Division of Cardiology, Department of Medicine, Gainesville, FL, United States of America. FAU - Cui, Yujie AU - Cui Y AD - Cedars-Sinai Medical Center, Biostatistics and Bioinformatics Research Center, Los Angeles, CA, United States of America. FAU - Takahashi, Tatsunori AU - Takahashi T AD - Cedars-Sinai Medical Center, Department of Cardiology, Smidt Heart Institute, Los Angeles, CA, United States of America. FAU - Handberg, Eileen AU - Handberg E AD - University of Florida, Division of Cardiology, Department of Medicine, Gainesville, FL, United States of America. FAU - Pepine, Carl J AU - Pepine CJ AD - University of Florida, Division of Cardiology, Department of Medicine, Gainesville, FL, United States of America. FAU - Merz, C Noel Bairey AU - Merz CNB AD - Cedars-Sinai Medical Center, Barbra Streisand Women's Heart Center, Smidt Heart Institute, Los Angeles, CA, United States of America. FAU - Nelson, Michael D AU - Nelson MD AD - The University of Texas at Arlington, Department of Kinesiology, Arlington, TX, United States of America; Cedars-Sinai Medical Center, Barbra Streisand Women's Heart Center, Smidt Heart Institute, Los Angeles, CA, United States of America. Electronic address: michael.nelson3@uta.edu. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Int J Cardiol JT - International journal of cardiology JID - 8200291 SB - IM OTO - NOTNLM OT - ANOCA OT - Coronary vascular dysfunction OT - Diastolic dysfunction OT - Diastolic reserve OT - HFPEF OT - Isometric handgrip stress COIS- Declaration of competing interest APO is employed by CD Leycom. JW: None B Samuels: Consultant/Speakers Bureau for Abbott Vascular and Philips. Advisory board member for Vehaticor. TJS: None. B Sharif: None. BA: None. JWP: None. YC: None. TT: None. EH: None. CJP: None. CNBM: Board of directors and receives stock, iRhythm. Research grants from Phillips, Abbott Diagnostics. MDN: None. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:32 PHST- 2026/09/23 00:00 [received] PHST- 2026/10/23 00:00 [revised] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:32 [entrez] AID - S0167-5273(26)00881-8 [pii] AID - 10.1016/j.ijcard.2026.135031 [doi] PST - aheadofprint SO - Int J Cardiol. 2026 Oct 28:135031. doi: 10.1016/j.ijcard.2026.135031. PMID- 42951022 OWN - NLM STAT- Publisher LR - 20261028 IS - 2452-0748 (Electronic) IS - 2452-0748 (Linking) DP - 2026 Oct 28 TI - Evaluation of the environmental fate and toxicity of product-released and exposed engineered nanomaterials from commercial products in aquatic systems. PG - 100650 LID - S2452-0748(26)00040-6 [pii] LID - 10.1016/j.impact.2026.100650 [doi] AB - The utilisation of nano-enabled products (NEPs) can lead to the release of engineered nanomaterials (ENMs) into aquatic systems, where they undergo transformations upon interacting with other substances in environmental compartments. These exposed product-released (PR)-ENMs pose a potential risk to organisms and may adversely affect the ecosystem. In this study, ten commercially available day-to-day products (sunscreens, soaps, creams, toothpaste, and gel) were evaluated, including the release behaviour of ENMs in aqueous media (using four representative commercial products). This included physicochemical properties and toxicity, assessed using the zebrafish embryo development test (ZFET). Among the ten selected NEPs, titanium dioxide (nTiO(2)) exhibited angular, elongated, spherical, and rectangular shapes, with particle sizes ranging from 37.7 x 16.1 nm to 99.3 x 62.0 nm (length x width). At the same time, zinc oxide (nZnO) displayed angular, irregular, and hexagonal shapes with a particle size range of 38.1 x 18.4 nm to 125.4 x 69.4 nm. The total detected concentrations of Ti and Zn were 0.08% to 19.4% and 1.95 x 10(-3)% to 3.6%, respectively, while Ag, which was also expected in NEPs, was not detected. The NEPs released an agglomerated, angular, elongated, and spherical shape of nTiO(2) (0.011% to 0.049%), while in nZnO (9.0 x 10(-4)% to 0.012%), angular and rectangular shapes were released. The developmental toxicity of ENMs to zebrafish embryos was dose-dependent, with exposed PR-ENMs causing greater adverse effects on hatching rate and mortality than PR-ENMs. The study identified key characteristics of ENMs, their release mechanisms, and potential toxicity, thereby demonstrating the ecological risks associated with PR-ENMs and their environmentally driven alterations. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Mzinyati, T E AU - Mzinyati TE AD - Department of Chemical Sciences, University of Johannesburg, Johannesburg 2006, South Africa. FAU - Thwala, M AU - Thwala M AD - Academy of Science of South Africa, Lynwood, Pretoria 0001, South Africa. FAU - Odermatt, B AU - Odermatt B AD - Institute of Anatomy, Medical Faculty, University of Bonn, Bonn, Germany. FAU - Lehutso, R F AU - Lehutso RF AD - Water Research Centre, Council for Scientific and Industrial Research, Pretoria 0001, South Africa. FAU - Seopela, M P AU - Seopela MP AD - Department of Chemical Sciences, University of Johannesburg, Johannesburg 2006, South Africa. Electronic address: mpseopela@uj.ac.za. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - NanoImpact JT - NanoImpact JID - 101676795 SB - IM OTO - NOTNLM OT - Engineered nanomaterials OT - Environmental fate OT - Nano-enabled products OT - Toxicity assessment OT - Zebrafish (Danio rerio) OT - nTiO(2) OT - nZnO COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:32 PHST- 2026/06/14 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:32 [entrez] AID - S2452-0748(26)00040-6 [pii] AID - 10.1016/j.impact.2026.100650 [doi] PST - aheadofprint SO - NanoImpact. 2026 Oct 28:100650. doi: 10.1016/j.impact.2026.100650. PMID- 42951021 OWN - NLM STAT- Publisher LR - 20261028 IS - 2451-9030 (Electronic) IS - 2451-9022 (Linking) DP - 2026 Oct 28 TI - Altered Neural Oscillatory Coupling Reveals Cognitive Control Deficits with Hierarchical Characteristics and Emotional Specificity in Major Depressive Disorder. LID - S2451-9022(26)00316-2 [pii] LID - 10.1016/j.bpsc.2026.10.011 [doi] AB - BACKGROUND: Cognitive control deficits are a prevalent feature of major depressive disorder (MDD) and are influenced by emotional contexts. However, it remains unclear whether these deficits specifically exist at particular hierarchical levels of cognitive control and whether they are selectively exacerbated under distinct emotional contexts. Furthermore, the neural mechanisms underlying such deficits and their association with depressive symptoms remain to be further elucidated. METHODS: To fill these gaps, two experiments were conducted. In Experiment 1, 54 MDDs and 41 healthy controls (HCs) performed emotional and non-emotional hierarchical cognitive control tasks. Hierarchical demands were manipulated using low- and high-abstraction rules. Based on hierarchy- and emotion-specific deficits identified in Experiment 1, Experiment 2 (34 MDDs; 28 HCs) introduced rule memory set-size manipulation to test whether the deficit generalized to other hierarchical cognitive control subprocesses. Electroencephalography and eye-tracking were simultaneously recorded. Phase-amplitude coupling (PAC) quantified cross-frequency interactions subserving cognitive control. RESULTS: Delta-beta PAC was selectively reduced in MDDs compared with HCs during high-abstraction conditions under negative emotional contexts, but not in low-abstraction conditions or other contexts. Under the same conditions, MDDs also failed to show the typical memory-related increase in theta-gamma PAC. Moreover, emotion regulation scores significantly mediated the pathway from PAC abnormalities to depressive severity. CONCLUSIONS: Our findings indicate that altered neural oscillatory coupling reveals cognitive control deficits in MDD that exhibit both hierarchical characteristics and emotional specificity. These neural abnormalities may contribute to depressive severity through maladaptive emotion regulation strategies, providing a novel biological account of the pathophysiological mechanisms underlying MDD. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Zhang, Wenquan AU - Zhang W AD - Medical School, Tianjin University, Tianjin, 300072, China; State Key Laboratory of Advanced Medical Materials and Medical Devices, Tianjin, 300072, China. FAU - Gao, Ying AU - Gao Y AD - Mental Health Center, Tianjin Medical University, Tianjin, 300222, China. FAU - Li, Xinyao AU - Li X AD - Medical School, Tianjin University, Tianjin, 300072, China; State Key Laboratory of Advanced Medical Materials and Medical Devices, Tianjin, 300072, China. FAU - Li, Meijuan AU - Li M AD - Mental Health Center, Tianjin Medical University, Tianjin, 300222, China. FAU - Liu, Yuan AU - Liu Y AD - Mental Health Center, Tianjin Medical University, Tianjin, 300222, China. FAU - Li, Jie AU - Li J AD - Mental Health Center, Tianjin Medical University, Tianjin, 300222, China. FAU - Chen, Sitong AU - Chen S AD - Medical School, Tianjin University, Tianjin, 300072, China; State Key Laboratory of Advanced Medical Materials and Medical Devices, Tianjin, 300072, China; Haihe Laboratory of Brain-Computer Interaction and Human-Machine Integration, Tianjin, 300392, China. Electronic address: shuangliu@tju.edu.cn. FAU - Liu, Shuang AU - Liu S AD - Medical School, Tianjin University, Tianjin, 300072, China; State Key Laboratory of Advanced Medical Materials and Medical Devices, Tianjin, 300072, China; Haihe Laboratory of Brain-Computer Interaction and Human-Machine Integration, Tianjin, 300392, China. Electronic address: shuangliu@tju.edu.cn. FAU - Ming, Dong AU - Ming D AD - Medical School, Tianjin University, Tianjin, 300072, China; State Key Laboratory of Advanced Medical Materials and Medical Devices, Tianjin, 300072, China; Haihe Laboratory of Brain-Computer Interaction and Human-Machine Integration, Tianjin, 300392, China. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Biol Psychiatry Cogn Neurosci Neuroimaging JT - Biological psychiatry. Cognitive neuroscience and neuroimaging JID - 101671285 SB - IM OTO - NOTNLM OT - EEG OT - emotion OT - hierarchical cognitive control OT - major depressive disorder OT - phase-amplitude coupling EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:32 PHST- 2026/06/17 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:32 [entrez] AID - S2451-9022(26)00316-2 [pii] AID - 10.1016/j.bpsc.2026.10.011 [doi] PST - aheadofprint SO - Biol Psychiatry Cogn Neurosci Neuroimaging. 2026 Oct 28:S2451-9022(26)00316-2. doi: 10.1016/j.bpsc.2026.10.011. PMID- 42951018 OWN - NLM STAT- Publisher LR - 20261028 IS - 1942-5546 (Electronic) IS - 0025-6196 (Linking) DP - 2026 Oct 28 TI - Comparing SGLT2 Inhibitors on Incident Major Adverse Kidney Events in Type 2 Diabetes: A Target Trial Emulation. LID - S0025-6196(26)18817-X [pii] LID - 10.1016/j.mayocp.2026.09.025 [doi] AB - OBJECTIVE: To determine whether canagliflozin, dapagliflozin, and empagliflozin differ in rates of major adverse kidney events (MAKE) in adults with T2D at moderate cardiovascular risk and without stage 3+ chronic kidney disease (CKD) at baseline. PATIENTS AND METHODS: Using target trial emulation and U.S. claims data from commercial, Medicare Advantage, and traditional Medicare plans, we compared canagliflozin, dapagliflozin, and empagliflozin among adults >/= 21years with T2D at moderate cardiovascular risk (1-5% annualized MACE or death risk), without insulin use or stage 3+ chronic kidney disease (CKD) at baseline, initiating treatment from January 1, 2014, and December 31, 2021. Propensity scores estimated via super learner ensemble were incorporated as inverse probability weights into Cox models examining major adverse kidney events (MAKE: stage 3+ CKD, kidney failure, kidney replacement therapy initiation) and all-cause death under intention-to-treat analysis through December 31, 2022. RESULTS: The weighted cohort included 41,248, 17,548, and 72,638 adults initiating canagliflozin, dapagliflozin, and empagliflozin, respectively. One-year post-initiation, rates of MAKE were 3.2% in the dapagliflozin and empagliflozin arms and 3.5% in the canagliflozin arm; by three years, 8.4%, 8.5%, and 9.1%. Compared to canagliflozin, MAKE risk was lower with dapagliflozin (HR 0.91; 95%CI 0.85-0.97) and empagliflozin (HR 0.93; 95%CI 0.88-0.97), with no difference between dapagliflozin and empagliflozin. CONCLUSION: Empagliflozin and dapagliflozin were associated with modestly lower adverse kidney event rates compared to canagliflozin. These findings provide clinically actionable evidence to support selection of SGLT2i to reduce CKD risk in a highly prevalent subset of people with T2D. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Zehra, Anum AU - Zehra A AD - University of Maryland School of Medicine, Baltimore, MD, USA. FAU - Aktay, Sinan L AU - Aktay SL AD - University of Maryland School of Medicine, Baltimore, MD, USA. FAU - Deng, Yihong AU - Deng Y AD - Mayo Clinic Robert D. and Patricia E. Kern Center for the Science of Health Care Delivery, Rochester, MN, USA; Optum Labs, Eden Prairie, MN, USA. FAU - Swarna, Kavya Sindhu AU - Swarna KS AD - Mayo Clinic Robert D. and Patricia E. Kern Center for the Science of Health Care Delivery, Rochester, MN, USA; Optum Labs, Eden Prairie, MN, USA. FAU - Neumiller, Joshua J AU - Neumiller JJ AD - Department of Pharmacotherapy, Washington State University, Spokane, WA, USA; Providence Medical Research Center, Spokane, WA, USA. FAU - Polley, Eric C AU - Polley EC AD - Department of Public Health Sciences, University of Chicago, Chicago, IL, USA. FAU - Herrin, Jeph AU - Herrin J AD - Section of Cardiovascular Medicine, Department of Medicine, Yale School of Medicine, New Haven, CT, USA. FAU - Galindo, Rodolfo J AU - Galindo RJ AD - Division of Endocrinology, Department of Medicine, University of Miami Miller School of Medicine, Miami, FL, USA. FAU - Umpierrez, Guillermo E AU - Umpierrez GE AD - Division of Endocrinology, Department of Medicine, Emory University School of Medicine, Atlanta, GA, USA. FAU - Ross, Joseph S AU - Ross JS AD - Department of Internal Medicine, Yale School of Medicine, New Haven, CT, USA; Department of Health Policy and Management, Yale School of Public Health, New Haven, CT, USA. FAU - Brito, Juan P AU - Brito JP AD - Division of Endocrinology, Diabetes, Metabolism, and Nutrition, Mayo Clinic, Rochester, MN, USA; Knowledge and Evaluation Research Unit, Division of Endocrinology, Diabetes, Metabolism, and Nutrition, Department of Medicine, Mayo Clinic, Rochester, MN, USA. FAU - Montori, Victor M AU - Montori VM AD - Division of Endocrinology, Diabetes, Metabolism, and Nutrition, Mayo Clinic, Rochester, MN, USA; Knowledge and Evaluation Research Unit, Division of Endocrinology, Diabetes, Metabolism, and Nutrition, Department of Medicine, Mayo Clinic, Rochester, MN, USA. FAU - Mickelson, Mindy M AU - Mickelson MM AD - Mayo Clinic Robert D. and Patricia E. Kern Center for the Science of Health Care Delivery, Rochester, MN, USA. FAU - McCoy, Rozalina G AU - McCoy RG AD - Optum Labs, Eden Prairie, MN, USA; Division of Community Internal Medicine, Geriatrics, and Palliative Care, Department of Medicine, Mayo Clinic, Rochester, MN; University of Maryland Institute for Health Computing, North Bethesda, MD, USA; Division of Endocrinology, Diabetes, and Nutrition, Department of Medicine, University of Maryland School of Medicine, Baltimore, MD, USA. Electronic address: Rozalina.McCoy@som.umaryland.edu. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Mayo Clin Proc JT - Mayo Clinic proceedings JID - 0405543 SB - IM EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:32 PHST- 2026/05/21 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:32 [entrez] AID - S0025-6196(26)18817-X [pii] AID - 10.1016/j.mayocp.2026.09.025 [doi] PST - aheadofprint SO - Mayo Clin Proc. 2026 Oct 28:S0025-6196(26)18817-X. doi: 10.1016/j.mayocp.2026.09.025. PMID- 42951009 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-4626 (Electronic) IS - 1091-255X (Linking) DP - 2026 Oct 28 TI - Long-Term Pancreatic Function, Reconstruction Durability, and Disease Control After Pancreas-Preserving Total Duodenectomy: Systematic Review and Single-Arm Meta-Analysis. PG - 102652 LID - S1091-255X(26)00333-1 [pii] LID - 10.1016/j.gassur.2026.102652 [doi] AB - BACKGROUND: Pancreas-preserving total duodenectomy (PPTD) enables complete duodenal resection while preserving the pancreatic head in selected benign or premalignant disease. Long-term pancreatic function, reconstruction durability, and disease control after PPTD remain incompletely defined. METHODS: We performed a PRISMA-compliant systematic review and single-arm meta-analysis registered in PROSPERO. PubMed, Scopus, Web of Science, and the Cochrane Library were searched through June 29, 2026. Institutional reports were grouped into cohort families to avoid patient duplication. Pooled proportions were estimated using inverse-variance logit models or generalized linear mixed models for prespecified outcomes with zero events. Risk of bias and certainty of evidence were assessed using ROBINS-I and GRADE. RESULTS: Nine independent retrospective cohorts comprising 199 patients were included; 86.4% had familial adenomatous polyposis, and follow-up ranged from 20 to 125 months. Overall postoperative morbidity was 64.7% (95% CI, 51.5-76.0), major morbidity 40.3% (28.5-53.3), biliopancreatic anastomotic leakage 32.1% (23.5-42.1), and clinically relevant postoperative pancreatic fistula 27.5% (17.5-40.5). One early postoperative death was reported. New-onset diabetes and exocrine pancreatic insufficiency were each reported in 1.8%, and subsequent pancreatic resection occurred in 3.6%. Late pancreatitis occurred in 18.3%, recurrent pancreatitis in 12.4%, biliopancreatic stenosis in 5.0%, cholangitis in 3.3%, and late reoperation in 9.5%. Endoscopic surveillance was documented in 83.3%; jejunal adenomas occurred in 25.8%, additional jejunal resection in 4.8%, jejunal cancer in 2.5%, and gastric cancer in 4.6%. Certainty of evidence was very low across outcomes. CONCLUSIONS: PPTD is associated with substantial perioperative morbidity but low early mortality, with infrequently reported long-term endocrine and exocrine impairment, although no study applied functional testing. Late pancreatitis and metachronous jejunal disease remain clinically relevant and support lifelong endoscopic surveillance. Evidence remains limited to small retrospective series and does not establish comparative superiority over other surgical strategies. CI - Copyright (c) 2026 Society for Surgery of the Alimentary Tract. Published by Elsevier Inc. All rights reserved. FAU - Tafner, Philipe Franco do Amaral AU - Tafner PFDA AD - Department of Surgery, School of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil. FAU - de Brito, Giulia Trucolo AU - de Brito GT AD - Department of Surgery, School of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil. FAU - de Souza Bonfitto, Morgana Danubia Gomes AU - de Souza Bonfitto MDG AD - Department of Pathology, School of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil. FAU - de Almeida de Saito, Helena Paes AU - de Almeida de Saito HP AD - Department of Medical Oncology, School of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil. FAU - Gestic, Martinho Antonio AU - Gestic MA AD - Department of Surgery, School of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil. FAU - Utrini, Murillo Pimentel AU - Utrini MP AD - Department of Surgery, School of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil. FAU - Neto, Francisco Callejas AU - Neto FC AD - Department of Surgery, School of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil. FAU - Cazzo, Everton AU - Cazzo E AD - Department of Surgery, School of Medical Sciences, University of Campinas (UNICAMP), Campinas, SP, Brazil. Electronic address: notrevezzo@yahoo.com.br. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - Netherlands TA - J Gastrointest Surg JT - Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract JID - 9706084 SB - IM OTO - NOTNLM OT - Pancreas-preserving total duodenectomy OT - duodenal polyposis OT - familial adenomatous polyposis OT - meta-analysis OT - pancreas-sparing duodenectomy OT - pancreatic function EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:32 PHST- 2026/09/24 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:32 [entrez] AID - S1091-255X(26)00333-1 [pii] AID - 10.1016/j.gassur.2026.102652 [doi] PST - aheadofprint SO - J Gastrointest Surg. 2026 Oct 28:102652. doi: 10.1016/j.gassur.2026.102652. PMID- 42951007 OWN - NLM STAT- Publisher LR - 20261028 IS - 1532-2971 (Electronic) IS - 1090-0233 (Linking) DP - 2026 Oct 28 TI - A 3D Mitral Valve Interstitial Cell Model Recapitulates Selected Transcriptomic Signatures of Degenerative Mitral Valve Disease. PG - 106953 LID - S1090-0233(26)00410-7 [pii] LID - 10.1016/j.tvjl.2026.106953 [doi] AB - Degenerative mitral valve disease (DMVD) is a highly prevalent cardiovascular disorder in humans, dogs and pigs. However, the molecular mechanisms driving mitral valve interstitial cell (VIC) activation and extracellular matrix (ECM) remodeling remain incompletely understood. In this study, we established a three-dimensional (3D) VIC-collagen model (VIC-COL) to investigate the cellular changes of VIC activation and ECM remodeling in response to transforming growth factor-beta1 (TGF-beta1) stimulation. The results revealed that upregulation of alpha-smooth muscle actin (alpha-SMA), fibronectin and matrix metalloproteinase 2 (MMP2) was induced by TGF-beta1 in the VIC-COL model; subsequent transcriptomic profiling identified a significant phenotypic shift toward pro-inflammatory and matrix remodeling pathways. Crucially, comparative transcriptomic analysis with naturally occurred DMVD public datasets identified 120 shared Gene Ontology (GO) biological process pathways and 111 conserved differentially expressed genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis confirmed that TGF-beta1 treatment in the porcine in vitro model recapitulates a subset of the transcriptional features of DMVD, including the cell adhesion molecules, ECM-receptor interactions, tumor necrosis factor (TNF) signaling and cytokine-cytokine receptor interactions. Gene Set Enrichment Analysis (GSEA) further corroborated concordant Hallmark inflammatory signatures, including TNF-alpha signaling via nuclear factor-kappa B (NF-kappaB), and interleukin-6/Janus kinase/signal transducer and activator of transcription 3 (IL-6/JAK/STAT3) signaling. These shared transcriptomic features indicate that TGF-beta1 stimulation captures certain molecular aspects of ECM remodeling, VIC activation, and inflammatory-related signaling seen in naturally occurring DMVD. Collectively, the 3D VIC-COL system provides a reproducible in vitro platform for investigating selected molecular mechanisms of DMVD. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Zhao, Yuanjie AU - Zhao Y AD - School of Animal Science and Veterinary Medicine, Hainan University, Haikou 570228, China; School of Life and Health, Hainan University, Haikou 570228, China. FAU - Chen, Yu AU - Chen Y AD - School of Animal Science and Veterinary Medicine, Hainan University, Haikou 570228, China; Hainan International One Health Institute, Hainan University, Haikou, Hainan 570228, China; Hainan Province Key Laboratory of One Health, Haikou, Hainan 570228, China. FAU - Ding, Renbo AU - Ding R AD - School of Animal Science and Veterinary Medicine, Hainan University, Haikou 570228, China; Hainan International One Health Institute, Hainan University, Haikou, Hainan 570228, China; Hainan Province Key Laboratory of One Health, Haikou, Hainan 570228, China. FAU - Zhang, Miao AU - Zhang M AD - School of Animal Science and Veterinary Medicine, Hainan University, Haikou 570228, China; School of Life and Health, Hainan University, Haikou 570228, China. FAU - Chen, Shumin AU - Chen S AD - School of Animal Science and Veterinary Medicine, Hainan University, Haikou 570228, China. FAU - Tang, Qiyu AU - Tang Q AD - The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, EH25 9RG, United Kingdom. FAU - MacRae, Vicky E AU - MacRae VE AD - The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, EH25 9RG, United Kingdom; Centre for Biomedical Research and Health Innovation, School of Life Sciences, Anglia Ruskin University, Cambridge, CB1 1PT, United Kingdom. FAU - Zhu, Dongxing AU - Zhu D AD - Guangzhou Institute of Cardiovascular Disease, Guangdong Key Laboratory of Vascular Diseases, State Key Laboratory of Respiratory Disease, The Second Affiliated Hospital, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, Guangdong 510260, China. FAU - Liu, Mengmeng AU - Liu M AD - School of Animal Science and Veterinary Medicine, Hainan University, Haikou 570228, China; Hainan International One Health Institute, Hainan University, Haikou, Hainan 570228, China; Hainan Province Key Laboratory of One Health, Haikou, Hainan 570228, China. Electronic address: mmliu@hainanu.edu.cn. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Vet J JT - Veterinary journal (London, England : 1997) JID - 9706281 SB - IM OTO - NOTNLM OT - 3D hydrogel system OT - degenerative mitral valve disease OT - transcriptome analysis OT - valve interstitial cell COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Conflict of Interest The authors have no competing financial interests to declare. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:32 PHST- 2026/06/23 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:32 [entrez] AID - S1090-0233(26)00410-7 [pii] AID - 10.1016/j.tvjl.2026.106953 [doi] PST - aheadofprint SO - Vet J. 2026 Oct 28:106953. doi: 10.1016/j.tvjl.2026.106953. PMID- 42951003 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-176X (Electronic) IS - 0300-5712 (Linking) DP - 2026 Oct 28 TI - One-shade versus multi-shade flowable composites for non-carious cervical lesions: color match and 18-month clinical performance in a double-blind, split-mouth randomized trial. PG - 107139 LID - S0300-5712(26)00808-0 [pii] LID - 10.1016/j.jdent.2026.107139 [doi] AB - OBJECTIVES: This double-blind randomized clinical trial aimed to evaluate the objective color match and clinical performance of a one-shade flowable composite (Vittra APS Unique Flow, FGM) compared with a multi-shade flowable composite (Opallis Flow, FGM) in the restoration of non-carious cervical lesions (NCCLs). METHODS: A total of 124 NCCLs were restored in 62 participants (n = 62 restorations per material) in a randomized, split-mouth, multicenter design. Objective color parameters (DeltaE(ab) and DeltaE(00)) were assessed using a spectrophotometer by comparing the cervical third before and after restoration and the cervical and middle thirds after restoration at 7 days. Clinical performance was assessed using FDI criteria at baseline and after 6, 12, and 18 months. Data were analyzed using paired Student's t-tests and Wilcoxon signed-rank tests (alpha = 0.05). RESULTS: No significant differences between the one-shade and multi-shade composites were detected for DeltaE(ab) or DeltaE(00) in either objective color comparison (p > 0.05). At 18 months, retention rates were 98.4% for the one-shade composite and 100% for the multi-shade composite, with no significant difference between materials. No significant differences were observed for the remaining functional, biological, or esthetic FDI criteria, except color match (A3), for which the multi-shade composite showed a higher frequency of clinically excellent/very good scores (p = 0.008). All restorations evaluated for color match remained clinically acceptable. CONCLUSIONS: The one-shade flowable composite showed favorable 18-month clinical performance, with no significant differences in objective color measurements compared with the multi-shade composite. However, visual color matching favored the multi-shade material at 18 months, although all restorations evaluated for color match remained clinically acceptable. CLINICAL SIGNIFICANCE: One-shade flowable composites may simplify NCCL restorations by eliminating shade selection while maintaining favorable clinical performance and clinically acceptable color match. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved. FAU - Barbosa, Laryssa Mylenna Madruga AU - Barbosa LMM AD - Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa, Ponta Grossa, Parana, Brazil. Electronic address: laryssamylenna@hotmail.com. FAU - Favoreto, Michael Willian AU - Favoreto MW AD - Department of Restorative Dentistry, University of Sao Paulo, Sao Paulo, Sao Paulo, Brazil. Electronic address: michaelfavoreto@hotmail.com. FAU - Cochinski, Gabriel David AU - Cochinski GD AD - Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa, Ponta Grossa, Parana, Brazil. Electronic address: gabriel.d.cochinski@gmail.com. FAU - Monteles, Camila Farias AU - Monteles CF AD - Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa, Ponta Grossa, Parana, Brazil. Electronic address: camilafariasmonteles@hotmail.com. FAU - Rocha, Aurelio de Oliveira AU - Rocha AO AD - Department of Pediatric Dentistry, Federal University of Santa Catarina, Florianopolis, Santa Catarina, Brazil. Electronic address: aureliorocha2015@gmail.com. FAU - Dos Anjos, Lucas Menezes AU - Dos Anjos LM AD - Department of Pediatric Dentistry, Federal University of Santa Catarina, Florianopolis, Santa Catarina, Brazil. Electronic address: lucasanjos02@gmail.com. FAU - de Lima de Souza, Jacqueline AU - de Lima de Souza J AD - Department of Restorative Dentistry, Federal University of Parana, Curitiba, Parana, Brazil. Electronic address: souzajaqueline@ufpr.br. FAU - Matos, Thalita de Paris AU - Matos TP AD - Department of Restorative Dentistry, Federal University of Parana, Curitiba, Parana, Brazil. Electronic address: thalitamatos@live.com. FAU - Loguercio, Alessandro D AU - Loguercio AD AD - Department of Restorative Dentistry, School of Dentistry, State University of Ponta Grossa, Ponta Grossa, Parana, Brazil. Electronic address: aloguercio@hotmail.com. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - J Dent JT - Journal of dentistry JID - 0354422 SB - IM OTO - NOTNLM OT - Color match OT - Flowable composite OT - Non-carious cervical lesion OT - One-shade composite OT - Randomized clinical trial OT - Resin composite COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:32 PHST- 2026/09/19 00:00 [received] PHST- 2026/10/20 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:32 [entrez] AID - S0300-5712(26)00808-0 [pii] AID - 10.1016/j.jdent.2026.107139 [doi] PST - aheadofprint SO - J Dent. 2026 Oct 28:107139. doi: 10.1016/j.jdent.2026.107139. PMID- 42951002 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-176X (Electronic) IS - 0300-5712 (Linking) DP - 2026 Oct 28 TI - SHED in Regenerative Medicine: Cell-Based Therapies and Cell-Free Strategies. PG - 107141 LID - S0300-5712(26)00810-9 [pii] LID - 10.1016/j.jdent.2026.107141 [doi] AB - INTRODUCTION: Stem cells from human exfoliated deciduous teeth (SHED) have gained increasing attention in regenerative medicine. However, direct stem cell transplantation faces limitations such as cell survival, manufacturing consistency, genetic instability during expansion, immune compatibility, and potential tumorigenic risk. This review examines advances in SHED-based cell therapies as a biological and therapeutic foundation for emerging cell-free strategies, with emphasis on their mechanisms, regenerative applications, and translational challenges. DATA: A comprehensive literature search was conducted to identify studies focusing on SHED-based regeneration, SHED-derived cell-free components, including secretomes, extracellular vesicles (EVs), extracellular matrix (ECM), conditioned medium (CM), and decellularized matrices. SOURCES: The data sources included PubMed and Web of Science, encompassing peer-reviewed articles published in recent years regarding SHED and regenerative medicine. STUDY SELECTION: Studies were selected based on their relevance to SHED biological characteristics, cell-based therapeutic applications, and limitations of viable-cell transplantation, together with the mechanisms and regenerative applications of SHED-derived cell-free strategies. Preference was given to preclinical studies demonstrating the regenerative potential and clinical applicability of these alternatives to live cell therapies. CONCLUSIONS: Studies of SHED-based cell therapies support their regenerative potential and provide a biological foundation for developing cell-free strategies. Preclinical evidence indicates that SHED-derived products can support tissue repair through bioactive molecules and modulation of the host microenvironment. These emerging approaches may address some limitations of viable-cell transplantation, but standardized preparation, mechanistic validation, and further evaluation of safety and clinical efficacy are required for translation. CLINICAL SIGNIFICANCE: The transition to SHED-derived cell-free therapies marks a pivotal advancement in regenerative medicine. By harnessing selected functional activities of viable cells while avoiding associated risks, these therapies offer a "ready-to-use" therapeutic modality for tissue regeneration. With reproducible manufacturing, appropriate delivery strategies, and demonstrated safety and efficacy, they may enhance the precision and feasibility of regenerative dentistry and broader tissue repair. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Liu, Ying AU - Liu Y AD - Division of Paediatric Dentistry and Orthodontics, Faculty of Dentistry, University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR, China. FAU - Huang, Mengyu AU - Huang M AD - Division of Paediatric Dentistry and Orthodontics, Faculty of Dentistry, University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR, China. FAU - Dissanayaka, Waruna Lakmal AU - Dissanayaka WL AD - Division of Applied Oral Sciences & Community Dental Care, Faculty of Dentistry, University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR, China. FAU - Yiu, Cynthia AU - Yiu C AD - Division of Paediatric Dentistry and Orthodontics, Faculty of Dentistry, University of Hong Kong, 34 Hospital Road, Sai Ying Pun, Hong Kong SAR, China. Electronic address: ckyyiu@hku.hk. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - J Dent JT - Journal of dentistry JID - 0354422 SB - IM OTO - NOTNLM OT - cell-free regenerative therapy OT - conditioned medium OT - decellularized matrix OT - extracellular vesicles OT - secretome OT - stem cells derived from human exfoliated deciduous teeth OT - tissue regeneration COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:32 PHST- 2026/08/06 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:32 [entrez] AID - S0300-5712(26)00810-9 [pii] AID - 10.1016/j.jdent.2026.107141 [doi] PST - aheadofprint SO - J Dent. 2026 Oct 28:107141. doi: 10.1016/j.jdent.2026.107141. PMID- 42950993 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-2259 (Electronic) IS - 0168-9452 (Linking) DP - 2026 Oct 28 TI - Cellular identity dynamics during plant regeneration: lineage, state, and developmental competence. PG - 113555 LID - S0168-9452(26)00583-2 [pii] LID - 10.1016/j.plantsci.2026.113555 [doi] AB - Plant regeneration requires cells to alter developmental trajectories by reorganization of the existing cellular state, rather than uniform erasure of cellular identity. Increasing evidence from lineage tracing, single-cell transcriptomics, chromatin profiling and spatial approaches has revealed substantial heterogeneity and temporal separation in cellular properties. This review examines regeneration from four distinct properties of a cell: lineage history, molecular state, developmental competence, and fate, with positional information considered as a contextual constraint. Transcriptomic and chromatin studies show that pre-existing regulatory programs can be selectively repressed, retained or remodeled. Regenerative competence emerges from the interaction between this molecular state and contextual signals. We discuss how wound-associated signals, optimum stress, hormones, transcriptional networks, chromatin remodeling and cell-cycle dynamics reshape these properties over time in overlapping and context-dependent ways. We propose that regeneration can therefore involve temporary dissociation of lineage history, molecular state, competence, and fate, followed by their coordination during developmental stabilization. This provides a framework for understanding how cells retain aspects of their developmental history while acquiring the competence for new developmental fates and highlights the need for approaches that resolve these properties in the same cells over time. Distinguishing what a cell was, what state it occupies, what it can become, and what it ultimately becomes provides a framework for interpreting cellular identity dynamics during plant regeneration. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Thakur, Vidisha AU - Thakur V AD - Independent researcher Author Institution is Unverified, INDIA. Electronic address: vidisha.thakur30@gmail.com. FAU - Rane, Jagadish AU - Rane J AD - ICAR-Central Institute for Arid Horticulture Author Institution is Unverified. FAU - Pandey, Girish AU - Pandey G AD - Department of Bioscience and Biotechnology, Banasthali Vidyapith Author Institution is Unverified. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - Ireland TA - Plant Sci JT - Plant science : an international journal of experimental plant biology JID - 9882015 SB - IM OTO - NOTNLM OT - cell fate OT - cellular identity OT - chromatin OT - developmental competence OT - lineage history OT - plant regeneration OT - reprogramming OT - single-cell genomics OT - wound signaling COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Competing interests The authors declare that they have no competing interests EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:32 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:32 [entrez] AID - S0168-9452(26)00583-2 [pii] AID - 10.1016/j.plantsci.2026.113555 [doi] PST - aheadofprint SO - Plant Sci. 2026 Oct 28:113555. doi: 10.1016/j.plantsci.2026.113555. PMID- 42950988 OWN - NLM STAT- Publisher LR - 20261028 IS - 1520-6017 (Electronic) IS - 0022-3549 (Linking) DP - 2026 Oct 28 TI - Synergistic lubrication of trazodone hydrochloride by magnesium stearate and Syloid: Critical role of mixing sequence. PG - 104570 LID - S0022-3549(26)00418-1 [pii] LID - 10.1016/j.xphs.2026.104570 [doi] AB - Sticking of active pharmaceutical ingredients (APIs) to punches and dies remains a significant challenge in direct compression, particularly for highly adhesive compounds such as trazodone hydrochloride. This study investigated influence of lubricant type, premix design, and mixing sequence on lubrication efficiency and sticking mitigation. Binary mixtures containing trazodone hydrochloride and either magnesium stearate or amorphous porous silica (Syloid) were compared with ternary systems combining both excipients. Magnesium stearate provided effective boundary lubrication and markedly reduced sticking propensity, whereas Syloid alone showed poor lubrication efficiency and in some cases increased sticking behavior. Conversely, ternary systems exhibited strong dependence on the order of component incorporation. SEM/EDS analysis revealed Syloid can preferentially occupy API surfaces when introduced during early mixing stages, limiting access of magnesium stearate to critical interfacial regions and reducing lubrication efficiency. Conversely, premixing strategies favoring early adsorption of magnesium stearate promoted formation of effective lubrication layer and resulted in synergistically improved compaction performance. Surface etching experiments indicated pressure-dependent redistribution of magnesium stearate during compaction, consistent with its pronounced plastic deformability. Findings demonstrate lubrication efficiency is determined not only by lubricant selection but also by sequence-dependent microstructural organization established during premixing, highlighting premix design as a critical parameter for mitigating sticking. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Ludas, Radek AU - Ludas R AD - University of Chemistry and Technology Prague (UCT Prague), Department of Organic Technology, Technicka 5, Prague, Czech Republic. Electronic address: Radek.Ludas@vscht.cz. FAU - Kominova, Pavlina AU - Kominova P AD - University of Chemistry and Technology Prague (UCT Prague), Department of Organic Technology, Technicka 5, Prague, Czech Republic. Electronic address: Pavlina.Kominova@vscht.cz. FAU - Zamostny, Petr AU - Zamostny P AD - University of Chemistry and Technology Prague (UCT Prague), Department of Organic Technology, Technicka 5, Prague, Czech Republic. Electronic address: Petr.Zamostny@vscht.cz. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Pharm Sci JT - Journal of pharmaceutical sciences JID - 2985195R SB - IM OTO - NOTNLM OT - Compaction OT - Drug-excipient interaction(s) OT - Excipient(s) OT - Mechanical properties OT - Mixing OT - Powder technology(s) OT - Tableting COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:31 PHST- 2026/08/14 00:00 [received] PHST- 2026/10/26 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:31 [entrez] AID - S0022-3549(26)00418-1 [pii] AID - 10.1016/j.xphs.2026.104570 [doi] PST - aheadofprint SO - J Pharm Sci. 2026 Oct 28:104570. doi: 10.1016/j.xphs.2026.104570. PMID- 42950984 OWN - NLM STAT- Publisher LR - 20261028 IS - 1878-1810 (Electronic) IS - 1878-1810 (Linking) DP - 2026 Oct 28 TI - Deletion of TRPV4 in the POMC neurons exacerbates diet-induced obesity via Ca(2+)-dependent PGAM5-mediated mitophagy. LID - S1931-5244(26)00219-7 [pii] LID - 10.1016/j.trsl.2026.10.009 [doi] AB - Obesity is a major global health challenge driven by profound metabolic dysregulation. Hypothalamic pro-opiomelanocortin (POMC) neurons are indispensable for whole-body metabolic homeostasis, and transient receptor potential vanilloid 4 (TRPV4) channels have emerged as regulators of metabolic control. However, the cell-type-specific function of TRPV4 in POMC neurons remains poorly defined. Herein, we demonstrate a vital role of TRPV4 in regulating POMC neuronal activity. We found that hypothalamic TRPV4 was selectively upregulated in diet-induced obese mice, but remained unchanged in genetically obese mice. TRPV4 expression levels were influenced by nutritional status and exhibited co-localization with POMC. Mice lacking TRPV4 specifically in POMC neurons showed no alterations in systemic energy metabolism under chow diet conditions. However, under high-fat diet feeding, these mice exhibited exacerbated obesity and hyperphagia. In vitro assays revealed that TRPV4 activation impaired POMC neurons by promoting mitophagy. In contrast, TRPV4 inhibition restored POMC neuronal function. Mechanistically, TRPV4 compromised POMC neuronal activity by triggering Ca(2+)-dependent PGAM5 activation and enhancing PINK1/PARKIN-mediated mitophagy. These findings provide comprehensive insights into TRPV4 as a critical regulator of hypothalamic energy balance circuits and highlight its potential as a therapeutic target for metabolic disorders. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Xi, Pengjiao AU - Xi P AD - Division of Medical Technology, Tianjin Medical University, Tianjin, 300203, China. FAU - Li, Xuemin AU - Li X AD - Division of Medical Technology, Tianjin Medical University, Tianjin, 300203, China. FAU - Zhang, Yan AU - Zhang Y AD - Department of Clinical Laboratory Medicine, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230001, Anhui, China. FAU - Song, Xinrui AU - Song X AD - School of Public Health, Tianjin Medical University, Tianjin, 300070, China. FAU - Li, Silin AU - Li S AD - Division of Medical Technology, Tianjin Medical University, Tianjin, 300203, China. FAU - Wen, Jun AU - Wen J AD - Division of Medical Technology, Tianjin Medical University, Tianjin, 300203, China. FAU - Yuan, Na AU - Yuan N AD - Division of Medical Technology, Tianjin Medical University, Tianjin, 300203, China. FAU - Yu, Chunxia AU - Yu C AD - Division of Medical Technology, Tianjin Medical University, Tianjin, 300203, China. Electronic address: yuchunxia@tmu.edu.cn. FAU - Qi, Zhi AU - Qi Z AD - Department of Molecular Pharmacology, School of Medicine, Nankai University, Tianjin, 300071, China. Electronic address: qizhi@nankai.edu.cn. FAU - Shen, Yanna AU - Shen Y AD - Division of Medical Technology, Tianjin Medical University, Tianjin, 300203, China. Electronic address: shenyanna@tmu.edu.cn. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Transl Res JT - Translational research : the journal of laboratory and clinical medicine JID - 101280339 SB - IM OTO - NOTNLM OT - Obesity,.TRPV4,.POMC neuron,.Mitophagy,.PGAM5 COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:31 PHST- 2026/07/12 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:31 [entrez] AID - S1931-5244(26)00219-7 [pii] AID - 10.1016/j.trsl.2026.10.009 [doi] PST - aheadofprint SO - Transl Res. 2026 Oct 28:S1931-5244(26)00219-7. doi: 10.1016/j.trsl.2026.10.009. PMID- 42950982 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-1611 (Electronic) IS - 0022-1910 (Linking) DP - 2026 Oct 28 TI - Chemical diversification of juvenile hormones and receptor responses in representative hemiptera: Insights from comparison with a thysanopteran species. PG - 105093 LID - S0022-1910(26)00166-6 [pii] LID - 10.1016/j.jinsphys.2026.105093 [doi] AB - Juvenile hormone (JH) diversity and receptor ligand selectivity in hemipteran insects remain poorly understood. In this study, we surveyed the occurrence of juvenile hormone III (JH III) and juvenile hormone III skipped bisepoxide (JHSB(3)) in representative hemipteran species and a thysanopteran outgroup using ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) with authentic standards. JH III was detected in Thrips tabaci and Bemisia tabaci, whereas JHSB(3) and/or JH III were detected in the examined hemipteran species. Although the present analysis was qualitative and does not exclude the possibility of undetected low hormone titers, distinct JH profiles were repeatedly observed among lineages, indicating substantial diversity in JH occurrence within Hemiptera. To investigate whether this chemical diversity is accompanied by changes in receptor function, we examined ligand-dependent interactions between the JH receptor Methoprene-tolerant (Met) and its cofactor Taiman (Tai) using a mammalian two-hybrid assay. Met proteins exhibited species-specific response types: JH III-preferential, dual-responsive, and JHSB(3)-biased. Notably, receptor responsiveness did not always correspond to the endogenous JH profile of the species. Together, these findings reveal considerable diversification of both JH chemistry and receptor responsiveness across hemipteran lineages and support the hypothesis that diversification of JHs has been accompanied by evolutionary variation in receptor responses during the evolution of Condylognatha. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Tachibana, Hikaru AU - Tachibana H AD - Graduate School of Science, Osaka City University, Osaka, Japan. FAU - Furuta, Kenjiro AU - Furuta K AD - Division of Insect Advanced Technology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba, Japan. FAU - Kimura, Toshiyuki AU - Kimura T AD - Advanced Analysis Center, National Agriculture and Food Research Organization, Tsukuba, Japan. FAU - Watanabe, Kazuyo AU - Watanabe K AD - Division of Insect Advanced Technology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba, Japan. FAU - Takeda, Kazuki AU - Takeda K AD - Faculty of Science, Osaka Metropolitan University, Osaka, Japan. FAU - Kayukawa, Takumi AU - Kayukawa T AD - Division of Insect Advanced Technology, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Tsukuba, Japan. Electronic address: kayukawa.takumi815@naro.go.jp. FAU - Goto, Shin G AU - Goto SG AD - Graduate School of Science, Osaka City University, Osaka, Japan; Graduate School of Science, Osaka Metropolitan University, Osaka, Japan. Electronic address: shingoto@omu.ac.jp. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - J Insect Physiol JT - Journal of insect physiology JID - 2985080R SB - IM OTO - NOTNLM OT - Hemiptera OT - JHSB(3) OT - Juvenile hormone OT - Ligand binding OT - Methoprene-tolerant OT - Receptor selectivity OT - Thysanoptera COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Shin Goto reports financial support was provided by Japan Society for the Promotion of Science. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:31 PHST- 2026/04/11 00:00 [received] PHST- 2026/10/27 00:00 [revised] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:31 [entrez] AID - S0022-1910(26)00166-6 [pii] AID - 10.1016/j.jinsphys.2026.105093 [doi] PST - aheadofprint SO - J Insect Physiol. 2026 Oct 28:105093. doi: 10.1016/j.jinsphys.2026.105093. PMID- 42950978 OWN - NLM STAT- Publisher LR - 20261028 IS - 1096-7184 (Electronic) IS - 1096-7176 (Linking) DP - 2026 Oct 28 TI - An Integrated Strategy to Reprogram NRPS Initiation Modules for Long-Chain Surfactin Biosynthesis. PG - 102574 LID - S1096-7176(26)00169-2 [pii] LID - 10.1016/j.ymben.2026.102574 [doi] AB - Engineering of nonribosomal peptide synthetases (NRPSs) is frequently limited by loss of biosynthetic productivity following domain recombination, particularly within the initiation module, where interdomain compatibility plays a critical yet underexplored role. Although substrate selectivity can often be reprogrammed by replacing the starter condensation (Cs) domain, such modifications are typically accompanied by severe reductions in productivity, imposing a major bottleneck in NRPS engineering. To produce long-chain surfactin homologs, we replaced the native Cs domain of the surfactin synthetase SrfAA with a homologous Cs domain from a long-chain lipopeptide-producing NRPS. This enabled incorporation of long-chain fatty acyl substrates but resulted in a substantial decrease in production titer. Systematic analyses suggested that this productivity loss is primarily associated with suboptimal interdomain compatibility within the engineered initiation module rather than intrinsic deficiencies of the substituted Cs domain. By combining targeted mutagenesis of the Cs domain with interdomain engineering and fine-tuning of the carrier (T(1)) domain, we achieved recovery of production through combinatorial optimization. Notably, improved productivity was largely achieved without altering acyl-chain specificity, supporting the view that catalytic efficiency can be enhanced independently of substrate selection. These findings suggest that productive reprogramming of NRPS initiation modules requires coordinated optimization of both domain-specific properties and interdomain compatibility. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Kobayashi, Shingo AU - Kobayashi S AD - Agri-Bio Research Center, KANEKA CORPORATION, 1-8, Miyamae-cho, Takasago-cho, Takasago-city, Hyogo 676-8688, Japan. Electronic address: Shingo.Kobayashi@kaneka.co.jp. FAU - Katsuyama, Yohei AU - Katsuyama Y AD - Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan; Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan. FAU - Matsui, Misato AU - Matsui M AD - Agri-Bio Research Center, KANEKA CORPORATION, 1-8, Miyamae-cho, Takasago-cho, Takasago-city, Hyogo 676-8688, Japan. FAU - Aoki, Rina AU - Aoki R AD - Agri-Bio Research Center, KANEKA CORPORATION, 1-8, Miyamae-cho, Takasago-cho, Takasago-city, Hyogo 676-8688, Japan. FAU - Matsumoto, Riku AU - Matsumoto R AD - Agri-Bio Research Center, KANEKA CORPORATION, 1-8, Miyamae-cho, Takasago-cho, Takasago-city, Hyogo 676-8688, Japan. FAU - Asamizu, Shumpei AU - Asamizu S AD - Engineering Biology Research Center, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan. FAU - Tanaka, Kenya AU - Tanaka K AD - Engineering Biology Research Center, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan. FAU - Bamba, Takahiro AU - Bamba T AD - Engineering Biology Research Center, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan. FAU - Ohshima, Kanji AU - Ohshima K AD - Bio-Pharma Research Laboratories, KANEKA CORPORATION, 1-8, Miyamae-cho, Takasago-cho, Takasago-city, Hyogo 676-8688, Japan. FAU - Hasunuma, Tomohisa AU - Hasunuma T AD - Engineering Biology Research Center, Kobe University, 1-1 Rokkodai, Nada, Kobe, 657-8501, Japan; Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan; RIKEN Center for Sustainable Resource Science, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan. FAU - Ohnishi, Yasuo AU - Ohnishi Y AD - Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan; Collaborative Research Institute for Innovative Microbiology, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan. LA - eng PT - Journal Article DEP - 20261028 PL - Belgium TA - Metab Eng JT - Metabolic engineering JID - 9815657 SB - IM OTO - NOTNLM OT - Bacillus subtilis OT - Cyclic lipopeptide OT - Nonribosomal peptide synthetase OT - Starter condensation domain OT - Surfactin EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:31 PHST- 2026/09/18 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:31 [entrez] AID - S1096-7176(26)00169-2 [pii] AID - 10.1016/j.ymben.2026.102574 [doi] PST - aheadofprint SO - Metab Eng. 2026 Oct 28:102574. doi: 10.1016/j.ymben.2026.102574. PMID- 42950975 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-1570 (Electronic) IS - 0300-9572 (Linking) DP - 2026 Oct 28 TI - Traumatic Emergency Thoracotomy for Resuscitation in Shock (TETRiS): a national service evaluation of resuscitative thoracotomy practice outside the operating theatre. PG - 111371 LID - S0300-9572(26)02418-4 [pii] LID - 10.1016/j.resuscitation.2026.111371 [doi] AB - BACKGROUND: Resuscitative thoracotomy (RT) is performed in patients with traumatic cardiac arrest or peri-arrest to treat potentially reversible causes of circulatory collapse. Optimal patient selection and timing remain uncertain, and existing evidence is largely retrospective, often excluding prehospital deaths. This prospective service evaluation examined contemporary RT practice and outcomes across a national major trauma system, including prehospital and emergency department settings. METHODS: Prospective 12-month observational study across 20 UK Helicopter Emergency Medical Services and 26 Major Trauma Centre Emergency Departments, using the PHOTON, TERN, and NaTRIC research networks. RESULTS: A total of 116 patients underwent RT, of which 82 (70.7%) were performed prehospital. Median age was 30 years, 97 patients (83.6%) were male, and 81 (69.8%) sustained penetrating injury. Suspected cardiac tamponade was the most common indication for RT (72/116 [62.1%]), although operative findings demonstrated exsanguinating haemorrhage in 82 patients (70.7%). Overall, 50 patients (43.1%) achieved return of spontaneous circulation and eight (6.9%) survived to hospital discharge. Survival was strongly associated with pathophysiological cause, duration of arrest, and physiological state at the time of intervention, with substantially better outcomes observed in patients with cardiac tamponade than exsanguination. Patients undergoing emergency department RT demonstrated shorter arrest durations, more favourable physiology, and higher survival than those undergoing prehospital RT. There were no survivors beyond 10 minutes of traumatic cardiac arrest. CONCLUSION: In this prospective national evaluation of RT practice, outcomes were strongly associated with the pathophysiological cause and duration of arrest, and the residual physiology present at the time of intervention. The most favourable outcomes were observed in patients with cardiac tamponade and those undergoing RT before irreversible physiological deterioration. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Ramage, Lisa AU - Ramage L AD - Barts Health NHS Trust, London, UK; Essex & Herts Air Ambulance. FAU - Tucker, Harriet AU - Tucker H AD - St George's University Hospitals NHS Foundation Trust, London, UK. FAU - Marsden, Max Er AU - Marsden ME AD - Barts Health NHS Trust, London, UK; Centre for Trauma Sciences, Queen Mary University of London, London, UK. FAU - Wohlgemut, Jared M AU - Wohlgemut JM AD - Barts Health NHS Trust, London, UK; Centre for Trauma Sciences, Queen Mary University of London, London, UK. FAU - Naeem, Salman AU - Naeem S AD - Flinders Medical Centre, Adelaide, SA. FAU - Christian, Michael AU - Christian M AD - London's Air Ambulance, London, UK. FAU - Davenport, Ross AU - Davenport R AD - Barts Health NHS Trust, London, UK; Centre for Trauma Sciences, Queen Mary University of London, London, UK. FAU - Perkins, Zane B AU - Perkins ZB AD - Barts Health NHS Trust, London, UK; Centre for Trauma Sciences, Queen Mary University of London, London, UK; London's Air Ambulance, London, UK. Electronic address: zane.perkins2@nhs.net. CN - TETRiS Collaborative LA - eng PT - Journal Article DEP - 20261028 PL - Ireland TA - Resuscitation JT - Resuscitation JID - 0332173 SB - IM OTO - NOTNLM OT - Cardiac Tamponade OT - Haemorrhage OT - Resuscitative Thoracotomy OT - Trauma Systems OT - Traumatic Cardiac Arrest COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Zane Perkins reports a relationship with Queen Mary University of London that includes: funding grants. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. FIR - Abdelrehem, Eslam IR - Abdelrehem E FIR - Anderson, Rory IR - Anderson R FIR - Arbid, Dion IR - Arbid D FIR - Ashworth, Charlotte IR - Ashworth C FIR - Barker, Paul IR - Barker P FIR - Beattie, Scott IR - Beattie S FIR - Bennett, Jonathan IR - Bennett J FIR - Body, Richard IR - Body R FIR - Bootland, Duncan IR - Bootland D FIR - Boyle, Adrian IR - Boyle A FIR - Brookes, Andrew IR - Brookes A FIR - Brown, Luise IR - Brown L FIR - Burley, Duncan IR - Burley D FIR - Challen, Kirsty IR - Challen K FIR - Coutts, Amanda IR - Coutts A FIR - Crouch, Robert IR - Crouch R FIR - Eckersley, Tom IR - Eckersley T FIR - Evans, Amanda IR - Evans A FIR - Evans, Carl IR - Evans C FIR - Evans, Laura IR - Evans L FIR - Fletcher, John IR - Fletcher J FIR - Franklin, David IR - Franklin D FIR - Frost, Elizabeth IR - Frost E FIR - Fuller, Gordon IR - Fuller G FIR - Garrett, Justin IR - Garrett J FIR - Gough, Christopher IR - Gough C FIR - Greenhalgh, Rob IR - Greenhalgh R FIR - Griggs, Jo IR - Griggs J FIR - Hancock, Paul IR - Hancock P FIR - Hardwick, Susie IR - Hardwick S FIR - Harris, Amy IR - Harris A FIR - Harrison, Oliver IR - Harrison O FIR - Hazlerigg, Antonia IR - Hazlerigg A FIR - Holliday, Jonathan IR - Holliday J FIR - Horner, Daniel IR - Horner D FIR - Ihlenfeldt, Hadassah IR - Ihlenfeldt H FIR - Jackman, Daniel IR - Jackman D FIR - Jefferson, Oliver IR - Jefferson O FIR - Jones, Jonathan IR - Jones J FIR - Karimi, Shayda IR - Karimi S FIR - Karra, Andriani IR - Karra A FIR - Kirby, Kim IR - Kirby K FIR - Knight, Katy IR - Knight K FIR - Lachowycz, Kate IR - Lachowycz K FIR - Lang, Nigel IR - Lang N FIR - Leech, Caroline IR - Leech C FIR - Lewis, Simon IR - Lewis S FIR - Lowe, David IR - Lowe D FIR - Luck, Hazel IR - Luck H FIR - Lunevicius, Raimundas IR - Lunevicius R FIR - Lutton, Stuart IR - Lutton S FIR - Major, Rob IR - Major R FIR - McCoy, Nigel IR - McCoy N FIR - McHenry, Ryan IR - McHenry R FIR - McKinlay, Jayne IR - McKinlay J FIR - McLachlan, Sarah IR - McLachlan S FIR - Monaghan, Darren IR - Monaghan D FIR - Moncur, Lyle IR - Moncur L FIR - Mullins, Nicola IR - Mullins N FIR - Murray, Elizabeth IR - Murray E FIR - Nash, Mark IR - Nash M FIR - Novak, Alex IR - Novak A FIR - Nutbeam, Tim IR - Nutbeam T FIR - O'Meara, Matthew IR - O'Meara M FIR - Paterson, Donna IR - Paterson D FIR - Pells, Greta IR - Pells G FIR - Percival, David IR - Percival D FIR - Pisirir, Erhan IR - Pisirir E FIR - Pountney, Andrew IR - Pountney A FIR - Procter, Richard IR - Procter R FIR - Quilty, Fionn IR - Quilty F FIR - Perks, James IR - Perks J FIR - Rodgers, Kenneth IR - Rodgers K FIR - Raitt, James IR - Raitt J FIR - Rawlinson, David IR - Rawlinson D FIR - Roberts, Tom IR - Roberts T FIR - Sadler, Jack IR - Sadler J FIR - Sattout, Abdo IR - Sattout A FIR - Shanahan, Thomas IR - Shanahan T FIR - Sharpe, Danny IR - Sharpe D FIR - Smith, Jason IR - Smith J FIR - Smith, Maria IR - Smith M FIR - Smith, Martin IR - Smith M FIR - Smith, Michael IR - Smith M FIR - Spowage-Delaney, Benjamin IR - Spowage-Delaney B FIR - Stevens, Joanna IR - Stevens J FIR - Studd, Neil IR - Studd N FIR - Taylor, Matthew IR - Taylor M FIR - Thacker, Matthew IR - Thacker M FIR - Thomson, Kevin IR - Thomson K FIR - Thorburn, Joshua IR - Thorburn J FIR - Tonkins, Michael IR - Tonkins M FIR - Valentine, Ed IR - Valentine E FIR - Watson, Laura IR - Watson L FIR - Weeks, John IR - Weeks J FIR - Wilkins, Craig IR - Wilkins C FIR - Williams, Sarah IR - Williams S FIR - Worley, Daniel IR - Worley D EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:31 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:31 [entrez] AID - S0300-9572(26)02418-4 [pii] AID - 10.1016/j.resuscitation.2026.111371 [doi] PST - aheadofprint SO - Resuscitation. 2026 Oct 28:111371. doi: 10.1016/j.resuscitation.2026.111371. PMID- 42950972 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-6424 (Electronic) IS - 0269-7491 (Linking) DP - 2026 Oct 28 TI - Vehicle interiors as mobile reservoirs of urban microplastics: seasonal accumulation, polymer composition and occupant exposure inferred from car wash vacuum debris. PG - 129447 LID - S0269-7491(26)01817-8 [pii] LID - 10.1016/j.envpol.2026.129447 [doi] AB - Vehicle cabins are enclosed, plastic rich spaces that people occupy daily, yet the microplastics that accumulate inside them are rarely measured. Debris removed by car wash vacuum cleaners offers a direct and largely unexploited window on that accumulation and is itself an unmanaged urban waste stream. We quantified the abundance, morphology, colour and polymer composition of microplastics in vacuum debris from 15 formal car wash facilities in Mbombela (formerly Nelspruit), South Africa, spanning three land use zones (low density residential, central business district and industrial) and three seasons (hot-wet, cool-dry and hot-dry) in 2025, with 100 samples per season. Samples were density separated in zinc chloride (1.7 g cm(-3)), vacuum filtered, enumerated by stereomicroscopy (x80) and confirmed by Fourier transform infrared micro-spectroscopy. Microplastics were detected at every facility, although the proportion of individual samples with no detectable particles rose sharply in the hot-dry season, from 2.9 % to 74.3 % in the low density zone. Abundance peaked in the hot-wet season and was highest in the low density zone (4 480 +/- 3 260 particles kg(-1)). Fragments dominated, followed by fibres, foams, films and beads, and both morphotype and colour composition shifted with season and land use. Fourier-transform infrared micro-spectroscopy identified a polymer suite consistent with vehicle interior materials and occupant-derived debris, led by polydimethylsiloxane, poly (vinyl chloride) and poly (methyl methacrylate). Season explained substantially more variation in estimated occupant exposure than land use zone (partial eta(2) = 0.265-0.280 versus 0.014-0.019), and ingestion of settled dust accounted for 94-96 % of estimated annual fibre exposure against 4-6 % for inhalation. Car wash vacuum debris therefore functions both as an integrative indicator of the microplastics urban residents encounter inside vehicles and as an overlooked point source of secondary microplastic release where it is disposed of without containment. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Maluleke, Ndzivalelo C AU - Maluleke NC AD - Aquatic Systems Research group, School of Biology and Environmental Science, University of Mpumalanga, Nelspruit 1200, South Africa. FAU - Dalu, Tatenda AU - Dalu T AD - Centre for Invasion Biology, School for Climate Studies, Stellenbosch University, South Africa. FAU - Munyai, Linton F AU - Munyai LF AD - Aquatic Systems Research Group, Department of Water and Sanitation, University of Limpopo, Sovenga, 0727, South Africa. Electronic address: linton.munyai@ul.ac.za. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Environ Pollut JT - Environmental pollution (Barking, Essex : 1987) JID - 8804476 SB - IM OTO - NOTNLM OT - FTIR OT - South Africa OT - car wash vacuum debris OT - human exposure OT - microplastics OT - vehicle interior dust COIS- Declaration of Competing Interest ☒ The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Linton F Munyai reports financial support was provided by National Research Foundation. Linton F Munyai reports financial support was provided by Department of Higher Education and Training, South Africa. Tatenda Dalu reports financial support was provided by National Research Foundation. Celebration Maluleke reports financial support was provided by Mining Qualification Authority. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:31 PHST- 2026/08/17 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/10/28 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:31 [entrez] AID - S0269-7491(26)01817-8 [pii] AID - 10.1016/j.envpol.2026.129447 [doi] PST - aheadofprint SO - Environ Pollut. 2026 Oct 28:129447. doi: 10.1016/j.envpol.2026.129447. PMID- 42950961 OWN - NLM STAT- Publisher LR - 20261028 IS - 1557-3117 (Electronic) IS - 1053-2498 (Linking) DP - 2026 Oct 28 TI - Cell and Gene Therapy in Lung Transplantation: From Experimental Promise towards Clinical Translation. LID - S1053-2498(26)02151-0 [pii] LID - 10.1016/j.healun.2026.10.021 [doi] AB - Lung transplantation (LTx) remains the only definitive therapy for selected patients with end-stage lung disease, yet outcomes are limited by early ischemia-reperfusion injury (IRI) and primary graft dysfunction (PGD), and by chronic lung allograft dysfunction (CLAD) as the leading cause of late graft failure. These entities share overlapping injury and repair pathways, including alveolocapillary barrier disruption, microvascular dysfunction, innate immune activation, and maladaptive remodeling, which remain incompletely controlled by current perioperative management and systemic immunosuppression. This review summarizes cell and gene therapy approaches in LTx and frames these strategies around the IRI/PGD-to-CLAD continuum. Stromal cell therapies, regulatory immune cell approaches, and cell-free or cell-derived products such as extracellular vesicles and mitochondrial transfer are discussed, with emphasis on route, dose, persistence, and the graft microenvironment as determinants of biological activity. Gene modulation strategies and delivery platforms relevant to the lung, including viral vectors, lipid nanoparticles, and virus-like particles, are outlined, together with preclinical gene therapy strategies targeting IRI and allograft rejection. Ex vivo lung perfusion (EVLP) is highlighted as a practical window for organ-confined delivery and early mechanistic assessment prior to implantation. Key translational considerations are outlined, including product characterization and potency, clinically interpretable dosing, and the need for harmonized endpoints and pharmacodynamic readouts to improve comparability across studies. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Olm, Franziska AU - Olm F AD - Wallenberg Center for Molecular Medicine, Lund University, Lund, Sweden; Department of Clinical Sciences, Lund University, Lund, Sweden; Lund Stem Cell Center, Lund University, Lund, Sweden. FAU - Kortleven, Pheline AU - Kortleven P AD - Laboratory of Respiratory Diseases and Thoracic Surgery, KU Leuven, Belgium. FAU - Mittendorfer, Margareta AU - Mittendorfer M AD - Wallenberg Center for Molecular Medicine, Lund University, Lund, Sweden; Department of Clinical Sciences, Lund University, Lund, Sweden; Lund Stem Cell Center, Lund University, Lund, Sweden. FAU - Carlon, Marianne S AU - Carlon MS AD - Laboratory of Respiratory Diseases and Thoracic Surgery, KU Leuven, Belgium; Leuven Viral Vector Core, KU Leuven, Belgium. FAU - Ceulemans, Laurens J AU - Ceulemans LJ AD - Laboratory of Respiratory Diseases and Thoracic Surgery, KU Leuven, Belgium; Department of Thoracic Surgery, University Hospitals Leuven, Belgium. Electronic address: laurens.ceulemans@uzleuven.be. FAU - Lindstedt, Sandra AU - Lindstedt S AD - Wallenberg Center for Molecular Medicine, Lund University, Lund, Sweden; Department of Clinical Sciences, Lund University, Lund, Sweden; Lund Stem Cell Center, Lund University, Lund, Sweden. Electronic address: sandra.lindstedt@med.lu.se. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - J Heart Lung Transplant JT - The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation JID - 9102703 SB - IM OTO - NOTNLM OT - Cell and gene therapy OT - Ex vivo lung perfusion (EVLP) OT - Ischemia-reperfusion injury (IRI) OT - Lung transplantation OT - Primary graft dysfunction (PGD) COIS- Conflicts of interest The authors declare no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper; SL serves as an Editorial Board Member for this journal and was not involved in the editorial review or the decision to publish this article. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:31 PHST- 2026/03/03 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:31 [entrez] AID - S1053-2498(26)02151-0 [pii] AID - 10.1016/j.healun.2026.10.021 [doi] PST - aheadofprint SO - J Heart Lung Transplant. 2026 Oct 28:S1053-2498(26)02151-0. doi: 10.1016/j.healun.2026.10.021. PMID- 42950954 OWN - NLM STAT- Publisher LR - 20261028 IS - 1437-7780 (Electronic) IS - 1341-321X (Linking) DP - 2026 Oct 28 TI - Successful Nonsurgical Management of Exophiala dermatitidis Infective Endocarditis Following Catheter-Related Bloodstream Infection: A Case Report and Literature Review. PG - 103117 LID - S1341-321X(26)00215-1 [pii] LID - 10.1016/j.jiac.2026.103117 [doi] AB - Fungal endocarditis (FE) caused by Exophiala dermatitidis (E. dermatitidis) is extremely rare, and optimal management remains unclear because most reported cases have been treated with combined surgical and antifungal therapy. We describe a 76-year-old man with catheter-related bloodstream infection complicated by tricuspid valve FE due to E. dermatitidis. The patient had a history of repeated catheter-related infections and was considered a poor candidate for surgery because of his general condition. After prompt removal of the implanted central venous port, he received sequential antifungal therapy with micafungin, liposomal amphotericin B, voriconazole, and long-term itraconazole suppression. Blood cultures cleared, and serial transthoracic echocardiography showed no clinically significant progression of the tricuspid vegetation. At more than 590 days after diagnosis, he remains alive without recurrent fungemia while receiving oral itraconazole. This case suggests that nonsurgical management with source control, systemic antifungal therapy guided by clinical response and antifungal susceptibility information, and long-term azole suppression may be a feasible option for selected patients with E. dermatitidis FE when surgery is not possible. CI - Copyright (c) 2026 Japanese Society of Chemotherapy, Japanese Association for Infectious Diseases, and Japanese Society for Infection Prevention and Control. Published by Elsevier Ltd. All rights reserved. FAU - Sato, Masaki AU - Sato M AD - Department of Nurse practitioner, Aichi Medical University Hospital, Nagakute, Aichi, Japan; Department of Clinical Infectious Diseases, Aichi Medical University Hospital, Nagakute, Aichi, Japan. FAU - Asai, Nobuhiro AU - Asai N AD - Department of Clinical Infectious Diseases, Aichi Medical University Hospital, Nagakute, Aichi, Japan; Department of Infection Prevention and Control, Aichi Medical University Hospital, Nagakute, Aichi, Japan. FAU - Ohno, Tomoko AU - Ohno T AD - Department of Infection Prevention and Control, Aichi Medical University Hospital, Nagakute, Aichi, Japan. FAU - Nakamura, Akiko AU - Nakamura A AD - Department of Infection Prevention and Control, Aichi Medical University Hospital, Nagakute, Aichi, Japan. FAU - Kawamoto, Yuzuka AU - Kawamoto Y AD - Department of Infection Prevention and Control, Aichi Medical University Hospital, Nagakute, Aichi, Japan. FAU - Takanyama, Mina AU - Takanyama M AD - Department of Infection Prevention and Control, Aichi Medical University Hospital, Nagakute, Aichi, Japan. FAU - Yamada, Atsuko AU - Yamada A AD - Department of Infection Prevention and Control, Aichi Medical University Hospital, Nagakute, Aichi, Japan. FAU - Shibata, Yuichi AU - Shibata Y AD - Department of Infection Prevention and Control, Aichi Medical University Hospital, Nagakute, Aichi, Japan. FAU - Matsumura, Tatsuki AU - Matsumura T AD - Department of Gastroenterological Surgery, Aichi Medical University Hospital, Nagakute, Aichi, Japan. FAU - Ohta, Hirotoshi AU - Ohta H AD - Department of Infection Prevention and Control, Aichi Medical University Hospital, Nagakute, Aichi, Japan. FAU - Mikamo, Hiroshige AU - Mikamo H AD - Department of Clinical Infectious Diseases, Aichi Medical University Hospital, Nagakute, Aichi, Japan; Department of Infection Prevention and Control, Aichi Medical University Hospital, Nagakute, Aichi, Japan; Department of Clinical Infectious Diseases, Asahi University Hospital, Gifu, Japan. Electronic address: mikamo@aichi-med-u.ac.jp. LA - eng PT - Case Reports PT - Journal Article DEP - 20261028 PL - Netherlands TA - J Infect Chemother JT - Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy JID - 9608375 SB - IM OTO - NOTNLM OT - Exophiala dermatitidis OT - catheter-related bloodstream infection OT - central venous port OT - fungal endocarditis OT - long-term azole suppressive therapy EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:31 PHST- 2026/07/06 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:31 [entrez] AID - S1341-321X(26)00215-1 [pii] AID - 10.1016/j.jiac.2026.103117 [doi] PST - aheadofprint SO - J Infect Chemother. 2026 Oct 28:103117. doi: 10.1016/j.jiac.2026.103117. PMID- 42950953 OWN - NLM STAT- Publisher LR - 20261028 IS - 2213-3348 (Electronic) IS - 2213-333X (Linking) DP - 2026 Oct 28 TI - Long-term outcomes of cyanoacrylate closure alone versus combined high saphenous ligation and cyanoacrylate closure in large-diameter great saphenous veins. PG - 102649 LID - S2213-333X(26)00209-X [pii] LID - 10.1016/j.jvsv.2026.102649 [doi] AB - OBJECTIVE: Long-term evidence regarding the effectiveness of cyanoacrylate closure (CAC) in large-diameter great saphenous veins (GSVs) is limited. This study aimed to compare the long-term outcomes of CAC alone with those of combined high saphenous ligation and cyanoacrylate closure (HSL + CAC) for large-diameter GSV insufficiency. METHODS: This single-center retrospective cohort study evaluated 198 extremities in 180 patients treated for symptomatic GSV insufficiency between January 2018 and December 2022 who had a preoperative GSV diameter of >/=8 mm. CAC was performed in 127 extremities and HSL + CAC in 71. Clinical and long-term duplex outcomes were compared using generalized estimating equation (GEE) models that accounted for within-patient clustering and prespecified confounders. Propensity score overlap weighting (PSOW) was used as a sensitivity analysis. RESULTS: Long-term duplex follow-up data were available for 186 extremities (93.9%). HSL + CAC was associated with greater GSV occlusion (84.1% vs 65.8%; P = .007), less saphenofemoral junction (SFJ) reflux (5.8% vs 47.0%; P < .001), a lower postoperative Venous Clinical Severity Score (VCSS; 2.45 +/- 2.04 vs 3.15 +/- 1.89; P = .019), and greater VCSS improvement (3.45 +/- 2.21 vs 1.47 +/- 1.93; P < .001). Reoperation was performed in 8.7% of extremities treated with CAC alone but in none treated with HSL + CAC (P = .008). In adjusted GEE analyses, HSL + CAC was associated with a lower postoperative VCSS (beta, -1.15; 95% CI, -1.71 to -0.59), greater GSV occlusion (OR, 3.05; 95% CI, 1.31-7.14), and less SFJ reflux (OR, 0.06; 95% CI, 0.015-0.223). PSOW estimates were consistent with the primary analyses. Complication rates were low and similar in both groups. CONCLUSIONS: In large-diameter GSVs, HSL + CAC was associated with better long-term anatomic and clinical outcomes than CAC alone, without an appreciable increase in complications. These findings suggest that adding HSL to CAC may improve long-term outcomes in selected patients with large-diameter GSVs. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Baltaci, Hakan AU - Baltaci H AD - Department of Cardiovascular Surgery, Elazig Fethi Sekin City Hospital, Elazig, Turkiye. Electronic address: hknbltc93@gmail.com. FAU - Duman, Zihni Mert AU - Duman ZM AD - Department of Cardiovascular Surgery, Pamukkale University Faculty of Medicine, Denizli, Turkiye. Electronic address: dumanzihnimert@gmail.com. FAU - Tacyildiz, Suna Yergin AU - Tacyildiz SY AD - Department of Radiology, Elazig Fethi Sekin City Hospital, Elazig, Turkiye. Electronic address: suna.yergin23@gmail.com. FAU - Tacyildiz, Celal AU - Tacyildiz C AD - Department of Radiology, Elazig Fethi Sekin City Hospital, Elazig, Turkiye. Electronic address: drcelaltacyildiz@gmail.com. FAU - Azboy, Davut AU - Azboy D AD - Department of Cardiovascular Surgery, Elazig Fethi Sekin City Hospital, Elazig, Turkiye. Electronic address: drazboy@gmail.com. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Vasc Surg Venous Lymphat Disord JT - Journal of vascular surgery. Venous and lymphatic disorders JID - 101607771 SB - IM OTO - NOTNLM OT - Cyanoacrylate closure OT - Great saphenous vein insufficiency OT - High saphenous ligation OT - Large-diameter vein OT - Saphenofemoral junction reflux EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:31 PHST- 2026/09/18 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:31 [entrez] AID - S2213-333X(26)00209-X [pii] AID - 10.1016/j.jvsv.2026.102649 [doi] PST - aheadofprint SO - J Vasc Surg Venous Lymphat Disord. 2026 Oct 28:102649. doi: 10.1016/j.jvsv.2026.102649. PMID- 42950942 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-3492 (Electronic) IS - 0009-8981 (Linking) DP - 2026 Oct 28 TI - Exploring patient-based real-time quality control: Insights from EWMA and simulation modeling. PG - 122948 LID - S0009-8981(26)02130-3 [pii] LID - 10.1016/j.cca.2026.122948 [doi] AB - BACKGROUND: Accurate clinical laboratory testing is vital for patient safety. This study compares patient-based real-time quality control (PBRTQC) methods, focusing on the Exponentially Weighted Moving Average (EWMA) algorithm against the Moving Average (MA) and Moving Median (MM) algorithms to optimize quality control through parameter tuning. METHODS: A simulation modeling study using nine months of historical data for 23 analytes evaluated PBRTQC performance. Data processing involved Box-Cox transformations to correct skewed distributions. Optimization targeted parameters like truncation limits, weight factors, and control limits. RESULTS: Screening monitoring objects improved stability and reduced false alarms. Box-Cox transformations outperformed extreme value exclusion. Optimizing the EWMA smoothing parameter (lambda) reduced false positives by >50% for 80% of analytes. EWMA showed superior sensitivity and specificity, especially for normally distributed data. CONCLUSION: PBRTQC methods can be optimized via simulations, enhancing clinical testing accuracy. Key optimization factors include monitoring object selection, data normalization, and parameter tuning. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Ying, Furong AU - Ying F AD - Department of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China. FAU - Zhao, Zhichao AU - Zhao Z AD - Yuyao People's Hospital, Yuyao, Zhejiang 315400, PR China. FAU - Chen, Wei AU - Chen W AD - Wenzhou Medical University, Wenzhou, Zhejiang 325000, PR China. FAU - Yang, Jianrong AU - Yang J AD - Department of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China. FAU - Vaishnani, Deep K AU - Vaishnani DK AD - Wenzhou Medical University, Wenzhou, Zhejiang 325000, PR China. FAU - Hla, Khaing Wut Yi AU - Hla KWY AD - Wenzhou Medical University, Wenzhou, Zhejiang 325000, PR China. FAU - Yang, Qiuqin AU - Yang Q AD - Wenzhou Medical University, Wenzhou, Zhejiang 325000, PR China. FAU - Zhao, Qiangwei AU - Zhao Q AD - Wenzhou Medical University, Wenzhou, Zhejiang 325000, PR China. FAU - Chen, Chen AU - Chen C AD - Wenzhou Medical University, Wenzhou, Zhejiang 325000, PR China. FAU - Zeng, Han AU - Zeng H AD - Wenzhou Medical University, Wenzhou, Zhejiang 325000, PR China. FAU - Chen, Han AU - Chen H AD - Department of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China. Electronic address: chenhan@wmu.edu.cn. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Clin Chim Acta JT - Clinica chimica acta; international journal of clinical chemistry JID - 1302422 SB - IM OTO - NOTNLM OT - Exponentially weighted moving average OT - Patient based real time quality control OT - Quality control OT - Simulation modeling COIS- Declaration of competing interest The authors report there are no competing interests to declare. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:30 PHST- 2026/02/23 00:00 [received] PHST- 2026/10/24 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:30 [entrez] AID - S0009-8981(26)02130-3 [pii] AID - 10.1016/j.cca.2026.122948 [doi] PST - aheadofprint SO - Clin Chim Acta. 2026 Oct 28:122948. doi: 10.1016/j.cca.2026.122948. PMID- 42950925 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-0003 (Electronic) IS - 0141-8130 (Linking) DP - 2026 Oct 28 TI - Selected metal-organic frameworks meet biopolymers: synthesis strategies and synergistic effects for biomedical applications. PG - 155029 LID - S0141-8130(26)04981-0 [pii] LID - 10.1016/j.ijbiomac.2026.155029 [doi] AB - Metal-organic frameworks (MOFs) offer high surface area, tunable porosity, and modular chemistry that support biomedical applications. Pristine metal-organic frameworks face aqueous instability and safety concerns; thus, incorporation with biopolymers provides a viable strategy to enhance structural integrity. Existing literature reviews primarily focus on specific material pairings or isolated applications. To provide a systematic perspective, this review evaluates synthesis strategies and synergistic mechanisms of metal-organic framework/biopolymer composites across drug delivery, wound healing, bone tissue engineering, and cancer theranostics. Fabrication pathways encompass in-situ growth on biopolymer matrices, covalently cross-linked networks, post-synthetic coatings, and template-guided morphological engineering. Biopolymers, including chitosan, alginate, gelatin, starch, and beta-cyclodextrin matrices, enhance mechanical stability and cytocompatibility. Concurrently, frameworks such as ZIF-8, MIL-53(Fe), MIL-101(Al), UiO-66, and HKUST-1 facilitate high therapeutic loading and responsive release. Biopolymer encapsulation functions as a physical diffusion barrier, mitigating initial burst release while enabling pH-responsive or redox-triggered kinetics under acidic pathological conditions. Surface functionalization preserves targeting capabilities and minimizes premature metal ion leaching. Composite dressings integrate antimicrobial activity with accelerated cellular migration for wound closure. Hydrogels and coated scaffolds promote osteogenesis and biomineralization in osteoblast cultures and rodent defect models. Furthermore, metal-organic framework/biopolymer composites enable combined chemotherapy, photothermal, or chemodynamic treatments alongside diagnostic imaging guidance. Preclinical evidence indicates that these hybrid materials address key stability limitations, offering adaptable material networks for therapeutic development. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Nguyen, Kien Gia AU - Nguyen KG AD - Nguyen Tat Thanh University Center for Hi-Tech Development, Saigon Hi-Tech Park, Ho Chi Minh City, Viet Nam; Institute of Applied Technology and Sustainable Development, Nguyen Tat Thanh University, Ho Chi Minh City, Viet Nam. FAU - Nguyen, Luan Minh AU - Nguyen LM AD - Institute of Advanced Technology, Vietnam Academy of Science and Technology, 1B TL29 Street, An Phu Dong Ward, Ho Chi Minh City, 700000, Viet Nam. FAU - Nguyen, Dai Hai AU - Nguyen DH AD - Institute of Advanced Technology, Vietnam Academy of Science and Technology, 1B TL29 Street, An Phu Dong Ward, Ho Chi Minh City, 700000, Viet Nam. FAU - Nguyen, Thuy Thi Thanh AU - Nguyen TTT AD - Nong Lam University Ho Chi Minh City, Ho Chi Minh City, 700000, Viet Nam. FAU - Nguyen, Duyen Thi Cam AU - Nguyen DTC AD - Nguyen Tat Thanh University Center for Hi-Tech Development, Saigon Hi-Tech Park, Ho Chi Minh City, Viet Nam; Institute of Applied Technology and Sustainable Development, Nguyen Tat Thanh University, Ho Chi Minh City, Viet Nam. Electronic address: ntcduyen@ntt.edu.vn. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - Netherlands TA - Int J Biol Macromol JT - International journal of biological macromolecules JID - 7909578 SB - IM OTO - NOTNLM OT - Biopolymer OT - Drug delivery OT - Metal-organic framework OT - Synthesis strategy OT - Wound healing COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:30 PHST- 2026/02/28 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:30 [entrez] AID - S0141-8130(26)04981-0 [pii] AID - 10.1016/j.ijbiomac.2026.155029 [doi] PST - aheadofprint SO - Int J Biol Macromol. 2026 Oct 28:155029. doi: 10.1016/j.ijbiomac.2026.155029. PMID- 42950920 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-0003 (Electronic) IS - 0141-8130 (Linking) DP - 2026 Oct 28 TI - Targeting mitogen-activated protein kinase 6-driven oncogenic signaling in lung adenocarcinoma: Insights from protein-ligand dynamics, conformational landscapes, and MM/PBSA binding energetics. PG - 155070 LID - S0141-8130(26)05023-3 [pii] LID - 10.1016/j.ijbiomac.2026.155070 [doi] AB - The atypical mitogen-activated protein kinase 6 (MAPK6), commonly referred to as ERK3, is a member of the MAP kinase family, plays a vital role in both the initiation and progression of cancer promotes the growth of tumors. Here, we used a multi-faceted in silico approach to identify potential phytochemical inhibitors of MAPK6. Using a curated collection of compounds from the IMPPAT 2.0 database, we utilized structure-based virtual screening methods to select several candidate phytochemicals. We then evaluated three selected candidates (Glabrocoumarin, Candidine, and Artonin P) using density functional theory (DFT) analysis and essential dynamics to assess their stability properties and interactions with the MAPK6 active site. To further support our assessment of the candidate phytochemicals' potential as MAPK6 inhibitors, we ran extended 1000 ns MD simulations to confirm conformational stability and the persistence of key intermolecular interactions. Overall, our analyses revealed strong binding interactions between the selected candidates and MAPK6, along with stable accommodations in the MAPK6 active site. Consequently, our analyses indicate that several plant-derived molecular scaffolds may be used as inhibitors of MAPK6. Additionally, our analyses support using computational approaches to evaluate the potential efficacy of phytochemicals as MAPK6 inhibitors. Ultimately, our analyses suggest that further experimental evaluation of the selected candidates will help identify effective therapeutics for treating lung cancer. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Sulaimani, Md Nayab AU - Sulaimani MN AD - Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, 110025, India. FAU - Ubaid-Ullah, Shah AU - Ubaid-Ullah S AD - Department of Biotechnology, Govt. Degree College, Pulwama, J & K Higher Education Department, Jammu and Kashmir, 192301, India. FAU - Alajmi, Mohamed F AU - Alajmi MF AD - Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia. FAU - Sohal, Sukhwinder Singh AU - Sohal SS AD - Centre for Respiratory Science and Health, School of Health and Biomedical Sciences, RMIT University, Melbourne, VIC, Australia. FAU - Dohare, Ravins AU - Dohare R AD - Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, 110025, India. Electronic address: ravins@jmi.ac.in. FAU - Hassan, Md Imtaiyaz AU - Hassan MI AD - Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, 110025, India. Electronic address: mihassan@jmi.ac.in. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Int J Biol Macromol JT - International journal of biological macromolecules JID - 7909578 SB - IM OTO - NOTNLM OT - Drug discovery OT - Lung adenocarcinoma OT - MD simulation OT - Mitogen-activated protein kinase 6 OT - Phytochemicals COIS- Declaration of competing interest The authors declare that they have no conflict of interest in the publication. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:30 PHST- 2026/09/20 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:30 [entrez] AID - S0141-8130(26)05023-3 [pii] AID - 10.1016/j.ijbiomac.2026.155070 [doi] PST - aheadofprint SO - Int J Biol Macromol. 2026 Oct 28:155070. doi: 10.1016/j.ijbiomac.2026.155070. PMID- 42950915 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-0003 (Electronic) IS - 0141-8130 (Linking) DP - 2026 Oct 28 TI - Construction of high amine density polymer‑sodium alginate based gel: Efficient and selective Cu(II) removal enabled by optimization strategy of interfacial compatibility and three-dimensional mass transfer network. PG - 155075 LID - S0141-8130(26)05028-2 [pii] LID - 10.1016/j.ijbiomac.2026.155075 [doi] AB - Traditional small-molecule amine-modified adsorbents suffer from insufficient free amine coordination sites, fragile 3D cross-linking networks and poor interfacial compatibility in Cu(II) wastewater treatment. Herein, a high-amine-density linear polymer polyethyleneimine (PEI) was incorporated into a graphene oxide/sodium alginate/montmorillonite (GO/SA/MMT) system via an induced self-assembly strategy to fabricate a composite gel PEI-GSM. PEI simultaneously supplies abundant free amine sites, promotes synergistic inter-chain cross-linking, and constructs a highly porous 3D interpenetrating network that provides ample mass transfer pathways and coordination sites for Cu(II). Our study provides an in-depth analysis of the structure-property relationship, interfacial regulation patterns and adsorption mechanisms of PEI-GSM gels, and compares their performance with that of small-molecule amine modification strategies in terms of enhancing Cu(II) coordination capacity, strengthening the cross-linked network and optimizing interfacial compatibility. PEI-GSM achieves 98.3% Cu(II) removal efficiency along with excellent reusability and anti-interference ability. Notably, the Cu(II)-loaded PEI-GSM serves as a visible-light photocatalyst for enrofloxacin degradation via .OH and .O(2)(-) radicals, realizing waste resource utilization and mitigating secondary pollution of spent adsorbents. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Wang, Haitao AU - Wang H AD - Liaoning Key Laboratory for Chemical Clean Production, Liaoning Key Laboratory for Surface Functionalization of Titanium Dioxide Powder, Institute Environmental Research, School of Materials and Environmental Engineering, Bohai University, Jinzhou, 121013, Liaoning, China. FAU - Li, Wenhui AU - Li W AD - Liaoning Key Laboratory for Chemical Clean Production, Liaoning Key Laboratory for Surface Functionalization of Titanium Dioxide Powder, Institute Environmental Research, School of Materials and Environmental Engineering, Bohai University, Jinzhou, 121013, Liaoning, China. FAU - Hao, Jie AU - Hao J AD - Liaoning Key Laboratory for Chemical Clean Production, Liaoning Key Laboratory for Surface Functionalization of Titanium Dioxide Powder, Institute Environmental Research, School of Materials and Environmental Engineering, Bohai University, Jinzhou, 121013, Liaoning, China. FAU - Wang, Yupeng AU - Wang Y AD - Liaoning Key Laboratory for Chemical Clean Production, Liaoning Key Laboratory for Surface Functionalization of Titanium Dioxide Powder, Institute Environmental Research, School of Materials and Environmental Engineering, Bohai University, Jinzhou, 121013, Liaoning, China. FAU - Song, Junyi AU - Song J AD - Liaoning Key Laboratory for Chemical Clean Production, Liaoning Key Laboratory for Surface Functionalization of Titanium Dioxide Powder, Institute Environmental Research, School of Materials and Environmental Engineering, Bohai University, Jinzhou, 121013, Liaoning, China. FAU - Niu, Yongman AU - Niu Y AD - Liaoning Key Laboratory for Chemical Clean Production, Liaoning Key Laboratory for Surface Functionalization of Titanium Dioxide Powder, Institute Environmental Research, School of Materials and Environmental Engineering, Bohai University, Jinzhou, 121013, Liaoning, China. FAU - Gu, Gaoyuan AU - Gu G AD - School of Energy and Environment, Southeast University, Nanjing, 210096, China. FAU - Bi, Changlong AU - Bi C AD - Liaoning Key Laboratory for Chemical Clean Production, Liaoning Key Laboratory for Surface Functionalization of Titanium Dioxide Powder, Institute Environmental Research, School of Materials and Environmental Engineering, Bohai University, Jinzhou, 121013, Liaoning, China. Electronic address: bichanglong@bhu.edu.cn. FAU - Zhang, Jingyuan AU - Zhang J AD - Liaoning Key Laboratory for Chemical Clean Production, Liaoning Key Laboratory for Surface Functionalization of Titanium Dioxide Powder, Institute Environmental Research, School of Materials and Environmental Engineering, Bohai University, Jinzhou, 121013, Liaoning, China. FAU - Yang, Shuyi AU - Yang S AD - Liaoning Key Laboratory for Chemical Clean Production, Liaoning Key Laboratory for Surface Functionalization of Titanium Dioxide Powder, Institute Environmental Research, School of Materials and Environmental Engineering, Bohai University, Jinzhou, 121013, Liaoning, China. FAU - Tao, E AU - Tao E AD - Liaoning Key Laboratory for Chemical Clean Production, Liaoning Key Laboratory for Surface Functionalization of Titanium Dioxide Powder, Institute Environmental Research, School of Materials and Environmental Engineering, Bohai University, Jinzhou, 121013, Liaoning, China. Electronic address: etao@bhu.edu.cn. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Int J Biol Macromol JT - International journal of biological macromolecules JID - 7909578 SB - IM OTO - NOTNLM OT - 3D interpenetrating network and interfacial compatibility enhancement OT - Confinement effect for Cu(II) selective removal OT - Sodium alginate-based gel adsorbent COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:30 PHST- 2026/08/29 00:00 [received] PHST- 2026/10/20 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:30 [entrez] AID - S0141-8130(26)05028-2 [pii] AID - 10.1016/j.ijbiomac.2026.155075 [doi] PST - aheadofprint SO - Int J Biol Macromol. 2026 Oct 28:155075. doi: 10.1016/j.ijbiomac.2026.155075. PMID- 42950911 OWN - NLM STAT- Publisher LR - 20261028 IS - 1532-8503 (Electronic) IS - 1051-2276 (Linking) DP - 2026 Oct 28 TI - Reframing Dietary Potassium Management in Chronic Kidney Disease: A Proportionate, Risk-Informed Approach for Multidisciplinary Practice. LID - S1051-2276(26)00198-6 [pii] LID - 10.1053/j.jrn.2026.09.005 [doi] AB - Dietary potassium restriction is a longstanding component of nutrition care in chronic kidney disease (CKD), yet its application often varies across practice settings and lacks consistent evidence for improved clinical outcomes. While clinicians routinely balance biochemical risk, nutritional adequacy, and patient context, the reasoning behind potassium-related decisions is rarely made visible in guidelines or multidisciplinary practice. This paper introduces the Proportionate Potassium Management Framework (PPMF), a practice-oriented model that supports transparent, risk-calibrated dietary decisions in CKD care. The framework prioritises systematic consideration of non-dietary contributors to hyperkalaemia and promotes selective, context-sensitive use of dietary restriction. Rather than prescribing uniform actions, the PPMF legitimises professional judgement as a core safety mechanism, offering a structure for documenting and justifying dietary decisions in complex, uncertain, or contested cases. In doing so, it aims to support nutritionally adequate, person-centred care without compromising biochemical safety or team accountability. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Morris, Andrew AU - Morris A AD - Health & Community Wellbeing Research Centre, Coventry University, Priory Street, CV1 5FB, United Kingdom; Worcestershire Acute Hospitals NHS Trust, Charles Hastings Way, Worcester, WR5 1DD, United Kingdom; Renal Theme, NIHR West Midlands Regional Research Delivery Network, United Kingdom. Electronic address: ac5129@coventry.ac.uk. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Ren Nutr JT - Journal of renal nutrition : the official journal of the Council on Renal Nutrition of the National Kidney Foundation JID - 9112938 SB - IM OTO - NOTNLM OT - Chronic kidney disease OT - Clinical decision-making OT - Dietary potassium OT - Hyperkalaemia management OT - Renal nutrition practice EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:30 PHST- 2026/01/15 00:00 [received] PHST- 2026/07/09 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:30 [entrez] AID - S1051-2276(26)00198-6 [pii] AID - 10.1053/j.jrn.2026.09.005 [doi] PST - aheadofprint SO - J Ren Nutr. 2026 Oct 28:S1051-2276(26)00198-6. doi: 10.1053/j.jrn.2026.09.005. PMID- 42950902 OWN - NLM STAT- Publisher LR - 20261028 IS - 1532-8406 (Electronic) IS - 0883-5403 (Linking) DP - 2026 Oct 28 TI - Clinical Outcomes of Fascia Iliaca Compartment Block Combined with Two Different Patient-Controlled Analgesia Regimens for Hip Arthroplasty: A Propensity Score-Matched Retrospective Observational Study. LID - S0883-5403(26)01116-2 [pii] LID - 10.1016/j.arth.2026.10.029 [doi] AB - BACKGROUND: The objective of our study was to retrospectively evaluate the analgesic efficacy, safety, and postoperative recovery of fascia iliaca compartment block combined with patient-controlled intravenous analgesia (FICB + PCIA) versus patient-controlled continuous nerve block analgesia (FICB + PCNB) in patients undergoing hip arthroplasty. METHODS: This single-center retrospective observational study was based on routinely collected clinical data. There were 136 patients (68 per group) who were selected via stratified random sampling from 1,500 eligible patients who underwent unilateral hip arthroplasty. A 1:1 nearest-neighbor propensity score matching (PSM) was performed with multiple clinical covariates to balance baseline characteristics. Standardized mean difference (SMD) was used to evaluate baseline balance after matching, with SMD less than 0.1 defined as good balance. The primary endpoint was postoperative resting Visual Analog Scale (VAS) scores at six, 12, 24, 48 and 72 hours. The secondary endpoints included 72-hour adverse events, analgesic consumption, lengths of hospital stay, and postoperative complications. RESULTS: All SMD of baseline covariates were below 0.1 after matching, with no intergroup differences. The VAS scores decreased significantly over time in both groups (F(time) = 22.846, P < 0.001). The FICB + PCNB group had lower overall resting VAS scores (F(group) = 4.974, P = 0.026), with no significant time-group interaction (F(interaction) = 1.728, P = 0.142). The total 72-hour adverse reaction rate was 10.29% in both groups. Opioid consumption was significantly lower in the FICB + PCNB group (P < 0.001). Duration of analgesic pump use, lengths of hospital stay, and complication rates were comparable between groups. CONCLUSION: The FICB + PCNB provides superior postoperative analgesia and reduces opioid consumption without increasing risks of adverse reactions or complications. It aligns with enhanced recovery after surgery principles and has favorable clinical application value. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Li, Man AU - Li M AD - Department of Anesthesiology, Sichuan Orthopedic Hospital, Chengdu 610041, Sichuan Province, China. FAU - Li, Yating AU - Li Y AD - The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010020, Inner Mongolia Autonomous Region, China. FAU - Zhou, Yin AU - Zhou Y AD - Department of Anesthesiology, Sichuan Orthopedic Hospital, Chengdu 610041, Sichuan Province, China. FAU - Qin, Zhijun AU - Qin Z AD - Department of Anesthesiology, Sichuan Orthopedic Hospital, Chengdu 610041, Sichuan Province, China. Electronic address: mrwupost@126.com. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Arthroplasty JT - The Journal of arthroplasty JID - 8703515 SB - IM OTO - NOTNLM OT - Fascia Iliaca Compartment Block OT - Hip Arthroplasty OT - Patient-Controlled Analgesia OT - Postoperative Pain OT - Propensity Score Matching EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:30 PHST- 2026/06/11 00:00 [received] PHST- 2026/10/20 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:30 [entrez] AID - S0883-5403(26)01116-2 [pii] AID - 10.1016/j.arth.2026.10.029 [doi] PST - aheadofprint SO - J Arthroplasty. 2026 Oct 28:S0883-5403(26)01116-2. doi: 10.1016/j.arth.2026.10.029. PMID- 42950887 OWN - NLM STAT- Publisher LR - 20261028 IS - 1476-5381 (Electronic) IS - 0007-1188 (Linking) DP - 2026 Oct 28 TI - GLP-1 receptor activation attenuates chronic migraine-like behaviour: Sex-specific modulation of opioid- and endocannabinoid-related pathways. LID - 10.1111/bph.70720 [doi] AB - BACKGROUND AND PURPOSE: Chronic migraine (CM) is a highly disabling condition that remains suboptimally treated. Metabolic disturbances may trigger migraine chronification, whereas impaired endogenous pain inhibition contributes to CM development. Glucagon-like peptide-1 receptor agonists (GLP-1RAs), used for type 2 diabetes and obesity, show efficacy in chronic pain models. We evaluated the prophylactic effects of liraglutide, semaglutide and dulaglutide compared with propranolol (reference drug), potential opioidergic/endocannabinoid involvement and sex differences in a CM model. EXPERIMENTAL APPROACH: Chronic migraine-like hypersensitivity was induced by repeated nitroglycerin administration in male and female mice. The prophylactic effects of GLP-1RAs and propranolol were examined using von Frey, acetone and orofacial glutamate tests. Body weight and glucose tolerance were monitored to evaluate metabolic safety. Potential opioidergic/endocannabinoid involvement in semaglutide-induced antinociception (selected GLP-1RA class representative) was assessed by pharmacological blockade and/or quantitative polymerase chain reaction (qPCR) analysis of pain-related neurobiomarker mRNA expression in brainstem and trigeminal ganglia. KEY RESULTS: Liraglutide, semaglutide and dulaglutide dose-dependently attenuated CM-like hypersensitivity in both sexes, with efficacy comparable to propranolol. GLP-1RAs did not affect metabolic parameters, whereas propranolol induced male-specific hyperglycaemia. Semaglutide-induced antinociception was partially sensitive to naloxone and was associated with sex-dimorphic, brainstem-specific transcriptional changes in opioid- and endocannabinoid-related pathways. Semaglutide increased Glp1r, Cnr1, Pomc, Pcsk1 and Pcsk2 mRNA expression in males, whereas it up-regulated Glp1r and down-regulated Faah and Mgll in females. CONCLUSIONS AND IMPLICATIONS: GLP-1RAs exert class-wide antinociceptive effects in a preclinical CM model, with semaglutide-based analyses suggesting sex-specific modulation of central opioid- and endocannabinoid-related pathways. These findings support further investigation of GLP-1RAs as prophylactic strategies for migraine, particularly in individuals with metabolic comorbidities. CI - (c) 2026 The Author(s). British Journal of Pharmacology published by John Wiley & Sons Ltd on behalf of British Pharmacological Society. FAU - Sicovic, Katarina AU - Sicovic K AUID- ORCID: 0009-0003-2441-1752 AD - Department of Pharmacology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia. FAU - Nastic, Katarina AU - Nastic K AUID- ORCID: 0009-0002-7052-7548 AD - Department of Pharmacology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia. FAU - Dinic, Miroslav AU - Dinic M AUID- ORCID: 0000-0001-5275-4531 AD - Group for Probiotics and Microbiota-Host Interaction, Department of Microbiology and Plant Biology, Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia. FAU - Brdaric, Emilija AU - Brdaric E AUID- ORCID: 0000-0002-5980-5394 AD - Group for Probiotics and Microbiota-Host Interaction, Department of Microbiology and Plant Biology, Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade, Belgrade, Serbia. FAU - Pecikoza, Uros AU - Pecikoza U AUID- ORCID: 0000-0002-2732-5978 AD - Department of Pharmacology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia. FAU - Tomic, Maja AU - Tomic M AUID- ORCID: 0000-0002-5178-7355 AD - Department of Pharmacology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia. FAU - Micov, Ana AU - Micov A AUID- ORCID: 0000-0003-1731-4631 AD - Department of Pharmacology, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia. LA - eng GR - 451-03-33/2026-03/200042/Ministry of Science, Technological Development, and Innovation of the Republic of Serbia/ GR - 451-03-33/2026-03/200161/Ministry of Science, Technological Development, and Innovation of the Republic of Serbia/ GR - 451-03-34/2026-03/200161/Ministry of Science, Technological Development, and Innovation of the Republic of Serbia/ PT - Journal Article DEP - 20261028 PL - England TA - Br J Pharmacol JT - British journal of pharmacology JID - 7502536 SB - IM OTO - NOTNLM OT - GLP-1 receptor agonists OT - chronic migraine OT - endocannabinoid pathways OT - opioidergic pathways OT - sex differences EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:12 PHST- 2026/09/10 00:00 [revised] PHST- 2026/04/08 00:00 [received] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:12 [entrez] AID - 10.1111/bph.70720 [doi] PST - aheadofprint SO - Br J Pharmacol. 2026 Oct 28. doi: 10.1111/bph.70720. PMID- 42950883 OWN - NLM STAT- Publisher LR - 20261028 IS - 1938-291X (Electronic) IS - 0022-0493 (Linking) DP - 2026 Oct 28 TI - Aggregation pattern, economic injury level, and sampling protocol for Chaetanaphothrips orchidii (Thysanoptera: Thripidae) in citrus orchards. LID - toag318 [pii] LID - 10.1093/jee/toag318 [doi] AB - Chaetanaphothrips orchidii (Moulton) is an invasive citrus pest in the Mediterranean, which has damaged up to 70% of fruit production. In this study, we aim to calculate its aggregation pattern, develop a sampling plan, and determine its economic injury levels (EIL). For these aims, 10 citrus orchards from Eastern Spain were sampled twice per month between 2018 and 2020, with sampling years varying among orchards. Both larvae and adults of C. orchidii tended to aggregate in fruits in contact with other fruits or leaves, in most orchards and years sampled. Fruit damage percentage increased with thrips occupation during the year. To monitor C. orchidii in citrus, we recommend sampling fruits in contact twice per month from June to October with either enumerative or binomial sampling. The manuscript provides an EIL calculator that considers management cost and revenue specific to each orchard. This tool estimated an EIL of 4.45% of fruit occupied by C. orchidii, for a standard Navel orange orchard sprayed with the maximum allowed concentration of acetamiprid. This sampling protocol and the EIL calculator will allow technicians and growers to decide when and whether an insecticide treatment is required. We expect these efforts to enhance the current integrated pest management program for C. orchidii in citrus orchards. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Junca-Morales, Carolina AU - Junca-Morales C AUID- ORCID: 0009-0002-9013-4936 AD - Centro de Proteccion Vegetal y Biotecnologia, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, Valencia, Spain. AD - Programa de Doctorado en Recursos y Tecnologias Agricolas, Universitat Politecnica de Valencia, Valencia, Spain. FAU - Catalan, Jose AU - Catalan J AD - Centro de Proteccion Vegetal y Biotecnologia, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, Valencia, Spain. FAU - Perez-Rodriguez, Jesica AU - Perez-Rodriguez J AD - Department of Genetics, Institute of Biotechnology and Biomedicine (BIOTECMED), Universitat de Valencia, Burjassot, Valencia, Spain. FAU - Monzo, Cesar AU - Monzo C AD - Centro de Proteccion Vegetal y Biotecnologia, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, Valencia, Spain. FAU - Tena, Alejandro AU - Tena A AUID- ORCID: 0000-0002-5001-4334 AD - Centro de Proteccion Vegetal y Biotecnologia, Instituto Valenciano de Investigaciones Agrarias (IVIA), Moncada, Valencia, Spain. LA - eng GR - Ministerio de Ciencia e Innovacion, and Fondo Social Europeo Plus/ PT - Journal Article DEP - 20261028 PL - England TA - J Econ Entomol JT - Journal of economic entomology JID - 2985127R SB - IM OTO - NOTNLM OT - IPM OT - anthurium thrips OT - grapefruit OT - sampling effort EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 20:03 PHST- 2026/03/12 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 20:03 [entrez] AID - 9004108 [pii] AID - 10.1093/jee/toag318 [doi] PST - aheadofprint SO - J Econ Entomol. 2026 Oct 28:toag318. doi: 10.1093/jee/toag318. PMID- 42950850 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1539-1612 (Electronic) IS - 1539-1604 (Print) IS - 1539-1604 (Linking) VI - 25 IP - 6 DP - 2026 Nov-Dec TI - An Inverse-Selection-Probability Weighted Approach for Real-World Progression-Free Survival With Left-Truncated Clinico-Genomic Data. LID - e70145 [pii] LID - 10.1002/pst.70145 [doi] AB - Next-generation sequencing (NGS) has become a vital tool in precision medicine, revolutionizing cancer diagnosis and treatment. However, in real-world studies where NGS defines study entry, data are often left-truncated because patients who do not survive until sequencing are excluded. Consequently, standard survival analysis techniques can result in biased, overestimated survival probabilities. While the commonly used risk set adjustment method addresses this bias, it excludes patients experiencing progression prior to sequencing, reducing statistical efficiency and power. We introduce a novel weighted approach utilizing the inverse of sample selection probability to include all observed patients. We also developed a computationally efficient, user-friendly method for calculating weights and estimating variance. The performance of the proposed method is evaluated through comprehensive simulation studies and further demonstrated using real-world data of metastatic breast cancer patients. The proposed weighted approach, utilizing inverse sample selection probabilities, ensures accurate parameter estimates and reliable inference. CI - (c) 2026 TEMPUS AI Inc. Pharmaceutical Statistics published by John Wiley & Sons Ltd. FAU - Liu, Yan AU - Liu Y AD - Tempus AI, Inc, Chicago, Illinois, USA. FAU - Hyun, Seung Won AU - Hyun SW AD - Tempus AI, Inc, Chicago, Illinois, USA. FAU - Sangli, Chithra AU - Sangli C AUID- ORCID: 0009-0000-1234-7122 AD - Tempus AI, Inc, Chicago, Illinois, USA. FAU - Beauchamp, Kyle AU - Beauchamp K AUID- ORCID: 0000-0001-6095-8788 AD - Tempus AI, Inc, Chicago, Illinois, USA. FAU - Islam, Farahnaz AU - Islam F AUID- ORCID: 0000-0002-8331-9871 AD - Tempus AI, Inc, Chicago, Illinois, USA. FAU - Shin, Dongho AU - Shin D AUID- ORCID: 0009-0006-5754-6815 AD - Tempus AI, Inc, Chicago, Illinois, USA. LA - eng PT - Journal Article PL - England TA - Pharm Stat JT - Pharmaceutical statistics JID - 101201192 SB - IM MH - Humans MH - Breast Neoplasms/genetics MH - Probability MH - Female MH - Progression-Free Survival MH - Computer Simulation MH - *High-Throughput Nucleotide Sequencing/methods/statistics & numerical data MH - *Genomics/methods/statistics & numerical data MH - Precision Medicine/methods PMC - PMC13716531 COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 PMCR- 2026/10/28 CRDT- 2026/10/28 19:52 PHST- 2026/10/06 00:00 [revised] PHST- 2026/04/15 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:52 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e70145 [pii] AID - PST70145 [pii] AID - 10.1002/pst.70145 [doi] PST - ppublish SO - Pharm Stat. 2026 Nov-Dec;25(6):e70145. doi: 10.1002/pst.70145. PMID- 42950833 OWN - NLM STAT- Publisher LR - 20261028 IS - 1555-9823 (Electronic) IS - 0003-1348 (Linking) DP - 2026 Oct 28 TI - Robotic Living Donor Hepatectomy After Previous Abdominal Surgery: Feasibility in a Carefully Selected Cohort. PG - 31348261504333 LID - 10.1177/00031348261504333 [doi] AB - BackgroundPrevious abdominal surgery (PAS) complicates open and laparoscopic living donor hepatectomy through intra-abdominal adhesions. Whether a robotic-intended strategy is feasible in donors with prior operations has not been described. A single-center experience in carefully selected donors is described.MethodsAll living donor hepatectomies at a single US center (August 2019 to December 2024) were reviewed. The primary intention-to-treat cohort comprised all 31 robotic-intended cases (13 PAS, 18 No-PAS), and the 28 cases intended for fully robotic completion served as a sensitivity analysis. PAS was classified as upper or lower abdominal. Exploratory comparisons appear as effect estimates with 95% confidence intervals.ResultsRobotic-intended hepatectomy was completed in all 13 PAS donors, 5 with prior upper abdominal surgery. Neither bile leak nor liver failure developed in a PAS donor, and adhesions accounted for no conversion. Blood loss, operative time, and hospital stay did not differ significantly, yet the confidence intervals stayed wide (hospital stay difference 1 day (95% CI -0.5 to 3)), so similarity cannot be established. Ileus affected 6 of 13 PAS donors and clustered in the upper abdominal subgroup, 4 of 5 (80.0%; 95% CI 28.4 to 99.5%); across all 12 events, 11 were Clavien-Dindo grade I.DiscussionAt an experienced center, a robotic-intended strategy proved feasible across varied previous operations. The donors described here were selected, so safety or equivalence cannot be established. Lower abdominal PAS may be managed routinely, whereas upper abdominal PAS calls for selective use in experienced programs together with prospective study. FAU - Hallesy, Jacob AU - Hallesy J AUID- ORCID: 0009-0005-1499-5162 AD - Department of Surgery, Virginia Commonwealth University School of Medicine, Richmond, VA, USA. RINGGOLD: 6886 FAU - Park, Andrew Min-Gi AU - Park AM AD - Department of Surgery, Virginia Commonwealth University School of Medicine, Richmond, VA, USA. RINGGOLD: 6886 FAU - Imai, Daisuke AU - Imai D AD - Division of Transplant Surgery, Department of Surgery, Hume-Lee Transplant Center, Virginia Commonwealth University Health System, Richmond, VA, USA. RINGGOLD: 6887 FAU - Sambommatsu, Yuzuru AU - Sambommatsu Y AD - Division of Transplant Surgery, Department of Surgery, Hume-Lee Transplant Center, Virginia Commonwealth University Health System, Richmond, VA, USA. RINGGOLD: 6887 FAU - Kumaran, Vinay AU - Kumaran V AD - Division of Transplant Surgery, Department of Surgery, Hume-Lee Transplant Center, Virginia Commonwealth University Health System, Richmond, VA, USA. RINGGOLD: 6887 FAU - Saeed, Muhammad AU - Saeed M AD - Division of Transplant Surgery, Department of Surgery, Hume-Lee Transplant Center, Virginia Commonwealth University Health System, Richmond, VA, USA. RINGGOLD: 6887 FAU - Khan, Aamir A AU - Khan AA AD - Division of Transplant Surgery, Department of Surgery, Hume-Lee Transplant Center, Virginia Commonwealth University Health System, Richmond, VA, USA. RINGGOLD: 6887 FAU - Sharma, Amit AU - Sharma A AD - Division of Transplant Surgery, Department of Surgery, Hume-Lee Transplant Center, Virginia Commonwealth University Health System, Richmond, VA, USA. RINGGOLD: 6887 FAU - Cotterell, Adrian H AU - Cotterell AH AD - Division of Transplant Surgery, Department of Surgery, Hume-Lee Transplant Center, Virginia Commonwealth University Health System, Richmond, VA, USA. RINGGOLD: 6887 FAU - Bruno, David A AU - Bruno DA AD - Division of Transplant Surgery, Department of Surgery, Hume-Lee Transplant Center, Virginia Commonwealth University Health System, Richmond, VA, USA. RINGGOLD: 6887 FAU - Lee, Seung Duk AU - Lee SD AD - Division of Transplant Surgery, Department of Surgery, Hume-Lee Transplant Center, Virginia Commonwealth University Health System, Richmond, VA, USA. RINGGOLD: 6887 LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Am Surg JT - The American surgeon JID - 0370522 SB - IM OTO - NOTNLM OT - adhesiolysis OT - intra-abdominal adhesions OT - living donor liver transplantation OT - previous abdominal surgery OT - robotic donor hepatectomy EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:42 PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:42 [entrez] AID - 10.1177/00031348261504333 [doi] PST - aheadofprint SO - Am Surg. 2026 Oct 28:31348261504333. doi: 10.1177/00031348261504333. PMID- 42950830 OWN - NLM STAT- Publisher LR - 20261028 IS - 2666-3791 (Electronic) IS - 2666-3791 (Linking) DP - 2026 Oct 28 TI - Moving beyond overconfidence: A clinical roadmap for integrating uncertainty-aware AI models into medical workflows. PG - 103124 LID - S2666-3791(26)00541-0 [pii] LID - 10.1016/j.xcrm.2026.103124 [doi] AB - Conventional artificial intelligence (AI) models can remain overconfident when their predictions are unreliable, limiting clinical adoption. Uncertainty quantification (UQ) supplements predictions with information about confidence, but the literature is often mathematically dense and difficult to translate into action. In this clinically oriented review, we synthesize current evidence and offer a practical perspective on the deployment of uncertainty-aware AI. We describe how uncertainty arises, summarize four principal computational approaches, and examine applications across clinical tasks, model development, foundation models, large language models, and agentic workflows. Uncertainty estimates may identify outputs requiring review, but they do not independently establish safety; their value depends on validation against relevant errors and linkage to appropriate actions in the intended setting. We, therefore, propose an adaptable roadmap connecting local reliability evaluation and threshold selection with subgroup assessment, prospective clinical evaluation, ongoing monitoring, and governance. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Inc. All rights reserved. FAU - Luo, Yilong AU - Luo Y AD - Joint Shantou International Eye Center, Shantou University & The Chinese University of Hong Kong, Shantou, China; Guangdong Engineering Technology Research Center of Precision Treatment for Ocular Diseases, Shantou, China; Guangdong Engineering Research Center of Intelligent Diagnosis and Treatment for Ocular Diseases, Shantou, China; Shantou Key Laboratory of Ocular Disease Prevention, Treatment, and Research, Shantou, China. FAU - Wang, Meng AU - Wang M AD - Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; Centre for Innovation and Precision Eye Health, Yong Loo Lin School of Medicine, National University of Singapore, Singapore. FAU - Lin, Tian AU - Lin T AD - Department of Ophthalmology, Shenzhen Nanshan People's Hospital and the 6th Affiliated Hospital of Shenzhen University Health Science Center, Shenzhen, China. FAU - Lin, Aidi AU - Lin A AD - Joint Shantou International Eye Center, Shantou University & The Chinese University of Hong Kong, Shantou, China; Guangdong Engineering Technology Research Center of Precision Treatment for Ocular Diseases, Shantou, China; Guangdong Engineering Research Center of Intelligent Diagnosis and Treatment for Ocular Diseases, Shantou, China; Shantou Key Laboratory of Ocular Disease Prevention, Treatment, and Research, Shantou, China. FAU - Chen, Yifan AU - Chen Y AD - Joint Shantou International Eye Center, Shantou University & The Chinese University of Hong Kong, Shantou, China; Guangdong Engineering Technology Research Center of Precision Treatment for Ocular Diseases, Shantou, China; Guangdong Engineering Research Center of Intelligent Diagnosis and Treatment for Ocular Diseases, Shantou, China; Shantou Key Laboratory of Ocular Disease Prevention, Treatment, and Research, Shantou, China. FAU - He, Guiqin AU - He G AD - Meizhou People's Hospital, Meizhou, China. FAU - Lin, Zhuanling AU - Lin Z AD - Joint Shantou International Eye Center, Shantou University & The Chinese University of Hong Kong, Shantou, China; Guangdong Engineering Technology Research Center of Precision Treatment for Ocular Diseases, Shantou, China; Guangdong Engineering Research Center of Intelligent Diagnosis and Treatment for Ocular Diseases, Shantou, China; Shantou Key Laboratory of Ocular Disease Prevention, Treatment, and Research, Shantou, China. FAU - Chen, Li Jia AU - Chen LJ AD - Joint Shantou International Eye Center, Shantou University & The Chinese University of Hong Kong, Shantou, China; Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China. FAU - Yam, Jason C AU - Yam JC AD - Joint Shantou International Eye Center, Shantou University & The Chinese University of Hong Kong, Shantou, China; Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China. FAU - Cheng, Ching-Yu AU - Cheng CY AD - Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore; Centre for Innovation and Precision Eye Health, Yong Loo Lin School of Medicine, National University of Singapore, Singapore. FAU - Pang, Chi Pui AU - Pang CP AD - Joint Shantou International Eye Center, Shantou University & The Chinese University of Hong Kong, Shantou, China; Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China. FAU - Tham, Clement C AU - Tham CC AD - Joint Shantou International Eye Center, Shantou University & The Chinese University of Hong Kong, Shantou, China; Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China. FAU - Fu, Huazhu AU - Fu H AD - Institute of Advanced Intelligence and Computing, Agency for Science, Technology and Research, Singapore. FAU - Chen, Haoyu AU - Chen H AD - Joint Shantou International Eye Center, Shantou University & The Chinese University of Hong Kong, Shantou, China; Guangdong Engineering Technology Research Center of Precision Treatment for Ocular Diseases, Shantou, China; Guangdong Engineering Research Center of Intelligent Diagnosis and Treatment for Ocular Diseases, Shantou, China; Shantou Key Laboratory of Ocular Disease Prevention, Treatment, and Research, Shantou, China; Department of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong SAR, China. Electronic address: haoyuchen@cuhk.edu.hk. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Cell Rep Med JT - Cell reports. Medicine JID - 101766894 SB - IM OTO - NOTNLM OT - clinical deployment OT - human-AI collaboration OT - medical artificial intelligence OT - uncertainty quantification COIS- Declaration of interests The authors declare no competing interests. Declaration of generative and AI-assisted technologies in the manuscript preparation process During the preparation of this manuscript, the authors used an LLM to refine grammar and language. After using this tool, the authors reviewed and edited the content as needed and take full responsibility for the final publication. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:42 PHST- 2026/03/28 00:00 [received] PHST- 2026/06/20 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:42 [entrez] AID - S2666-3791(26)00541-0 [pii] AID - 10.1016/j.xcrm.2026.103124 [doi] PST - aheadofprint SO - Cell Rep Med. 2026 Oct 28:103124. doi: 10.1016/j.xcrm.2026.103124. PMID- 42950818 OWN - NLM STAT- Publisher LR - 20261028 IS - 1097-4172 (Electronic) IS - 0092-8674 (Linking) DP - 2026 Oct 28 TI - Multimodal spatial transcriptomics redefines cortical pathology in Huntington's disease. LID - S0092-8674(26)01210-9 [pii] LID - 10.1016/j.cell.2026.09.064 [doi] AB - Huntington's disease (HD) is caused by CAG expansion in the huntingtin gene (HTT), yet how somatic repeat instability and HTT inclusions relate to selective cell loss in the human brain remains unclear. We have developed a multimodal spatial transcriptomics approach that enables the definition of transcriptional programs with subcellular resolution, somatic CAG repeat lengths, and eight other pathology markers, including HTT inclusions, in every cell of intact brain sections. Imaging 428,173 cells in the control and HD cortex revealed the selective vulnerability of layer 5-6 (L5-6) near-projecting (NP) neurons and L6b deep-layer excitatory neurons, which undergo a >50% loss that is closely linked to large somatic repeat expansions (>450 +/- 55 CAGs). Intranuclear inclusions were most prevalent at intermediate somatic repeat expansions (250-350 CAGs) and were associated with broader transcriptional changes than somatic repeat expansion alone. These data provide a comprehensive resource and establish a general framework for connecting repeat expansion and protein pathology across diverse cell types. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Vazquez-Sanchez, Sonia AU - Vazquez-Sanchez S AD - Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA. FAU - Arnold-Garcia, Olatz AU - Arnold-Garcia O AD - Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA; Department of Neurosciences, Biogipuzkoa Health Research Institute, 20014 San Sebastian, Spain; Centro de Investigacion Biomedica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), Carlos III Institute (ISCIII), Spanish Ministry of Sciences and Innovation, 28029 Madrid, Spain. FAU - Chillon-Marinas, Carlos AU - Chillon-Marinas C AD - Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA. FAU - Maimon, Roy AU - Maimon R AD - Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA; Department of Biomedical Engineering, New York University, Tandon School of Engineering, New York, NY 10003, USA. FAU - Cui, Jie AU - Cui J AD - Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA. FAU - McNair, Katelyn AU - McNair K AD - Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA. FAU - Dai, Yuanyi AU - Dai Y AD - Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA. FAU - Jimenez, Natalia L AU - Jimenez NL AD - Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA. FAU - Child, Daniel D AU - Child DD AD - Department of Laboratory Medicine and Pathology, University of Washington, Seattle, WA 98109, USA. FAU - Thompson, Leslie M AU - Thompson LM AD - Department of Neurobiology & Behavior, University of California, Irvine, Irvine, CA 92617, USA; Department of Psychiatry & Human Behavior, University of California, Irvine, Irvine, CA 92617, USA. FAU - Lopez-Erauskin, Jone AU - Lopez-Erauskin J AD - Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA. FAU - Bintu, Bogdan AU - Bintu B AD - Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA. Electronic address: bbintu@health.ucsd.edu. FAU - Cleveland, Don W AU - Cleveland DW AD - Department of Cellular and Molecular Medicine, University of California at San Diego, La Jolla, CA 92093, USA; Department of Neurosciences, University of California at San Diego, La Jolla, CA 92093, USA. Electronic address: dcleveland@health.ucsd.edu. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Cell JT - Cell JID - 0413066 SB - IM OTO - NOTNLM OT - Huntington's disease OT - multimodal spatial transcriptomics OT - neurodegeneration OT - protein aggregation OT - somatic repeat expansion OT - trinucleotide repeats COIS- Declaration of interests D.W.C. is a consultant for Ionis Pharmaceuticals. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:41 PHST- 2026/01/13 00:00 [received] PHST- 2026/05/13 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:41 [entrez] AID - S0092-8674(26)01210-9 [pii] AID - 10.1016/j.cell.2026.09.064 [doi] PST - aheadofprint SO - Cell. 2026 Oct 28:S0092-8674(26)01210-9. doi: 10.1016/j.cell.2026.09.064. PMID- 42950796 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-1506 (Electronic) IS - 0003-9969 (Linking) VI - 193 DP - 2026 Oct 23 TI - Animal orthodontic tooth movement models: A scoping review of model characteristics and reported outcomes. PG - 106789 LID - S0003-9969(26)00297-9 [pii] LID - 10.1016/j.archoralbio.2026.106789 [doi] AB - OBJECTIVE(S): To characterise the experimental designs, outcome assessments, and reporting practices used in animal models of orthodontic tooth movement (OTM). DESIGN: PubMed, Scopus, Embase via Ovid, and the Cochrane Central Register of Controlled Trials were searched on 8 May 2026. Two reviewers independently screened records and assessed eligibility. Studies applying active orthodontic force in vivo were included. Animal species, model configurations, loading parameters, outcome domains, and nine experimental reporting items were charted and synthesised descriptively. Reporting information was classified as reported (R), partially reported (P), not reported (NR), or not available (NA). RESULTS: The searches identified 2977 records, of which 1584 were screened and 269 underwent eligibility assessment; 247 studies were included. Rodents accounted for 235 studies (95.1%): 165 rat-only, 68 mouse-only, and two mixed rat-and-mouse studies. Tooth site and movement were chartable in 209 studies (84.6%), appliance or anchorage in 181 (73.3%), force in 160 (64.8%), and loading or observation time in 219 (88.7%). Among the nine reporting items, animal or group sample size was coded R in 103 studies (41.7%), random allocation in 66 (26.7%), and force verification in 29 (11.7%). CONCLUSIONS: Animal OTM research is dominated by rodent models and encompasses diverse loading protocols, experimental settings, and outcome measurements. Mapping these characteristics alongside reporting practices provides a structured reference for model selection, outcome assessment, and the design of reproducible animal OTM studies. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Huang, Xinqi AU - Huang X AD - State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China. FAU - Liao, Juntong AU - Liao J AD - State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China. FAU - Huang, Zhimao AU - Huang Z AD - School of Biomedical Engineering, University of New South Wales, Sydney, NSW, Australia. FAU - Cen, Xiao AU - Cen X AD - State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China. Electronic address: cenx@scu.edu.cn. LA - eng PT - Journal Article PT - Review DEP - 20261023 PL - England TA - Arch Oral Biol JT - Archives of oral biology JID - 0116711 SB - IM OTO - NOTNLM OT - Animal models OT - Bone remodeling OT - Orthodontic tooth movement OT - Periodontal ligament OT - Root resorption OT - Scoping review COIS- Declaration of Competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:13 PHST- 2026/09/21 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:13 [entrez] AID - S0003-9969(26)00297-9 [pii] AID - 10.1016/j.archoralbio.2026.106789 [doi] PST - aheadofprint SO - Arch Oral Biol. 2026 Oct 23;193:106789. doi: 10.1016/j.archoralbio.2026.106789. PMID- 42950794 OWN - NLM STAT- Publisher LR - 20261028 IS - 1090-2120 (Electronic) IS - 0045-2068 (Linking) VI - 184 DP - 2026 Oct 25 TI - C-4-functionalized cyclogeraniols from saffron: Structural diversity, Enantiomerism, and stereochemistry-dependent anti-melanogenic activity. PG - 110696 LID - S0045-2068(26)01232-0 [pii] LID - 10.1016/j.bioorg.2026.110696 [doi] AB - Crocus sativus L. (saffron) is known for its depigmenting and anti-melanogenic properties, yet the chemical diversity and bioactivity of its cyclogeraniol-derived (CGLs) minor metabolites remain insufficiently explored. In this study, phytochemical investigation of saffron stigmas led to the isolation of a series of C-4-functionalized cyclogeraniols, including six previously undescribed compounds, crosativoids A-F (1-6). Their structures and absolute configurations were elucidated by comprehensive spectroscopic analysis, single-crystal X-ray diffraction, and ECD calculations. These metabolites represent three distinct C-4 linkage patterns, namely CC, CN, and CO linkages. Among them, CGL 1 features a rare C-4C-C bond and an unprecedented 6/5/6/6 polycyclic framework, whereas CGLs (+/-)-2 and (+/-)-3 are cyclogeraniol hybrids bearing uracil and hypoxanthine-derived moieties, respectively. Chiral analysis of the CN and CO derivatives revealed intrinsic enantiomerism and suggested preferential formation of the C-4R configuration in saffron-derived cyclogeraniols. A plausible biosynthetic pathway involving C-4-selective nucleophilic addition was proposed. Biological evaluation showed that the 4R (-)-enantiomer of CGL 2 inhibited melanin accumulation in B16F10 cells and downregulated the MITF/TYR axis, with an SPR-derived affinity value of 455 nM toward tyrosinase. These findings expand the structural and stereochemical diversity of saffron cyclogeraniols and identify C-4-functionalized derivatives as promising anti-melanogenic candidates. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Li, Ting-Ting AU - Li TT AD - School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, PR China. FAU - Ji, Si-Bo AU - Ji SB AD - School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, PR China. FAU - Ma, Si-Qi AU - Ma SQ AD - School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, PR China. FAU - Zhang, Han-Qi AU - Zhang HQ AD - School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, PR China; Anhui Province Key Laboratory of Bioactive Natural Products, Hefei, 230012, PR China. FAU - Cai, Bai-Xiang AU - Cai BX AD - School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, PR China; Anhui Province Key Laboratory of Bioactive Natural Products, Hefei, 230012, PR China. FAU - Nuermaimaiti, Muaitaer AU - Nuermaimaiti M AD - Xinjiang Key Laboratory of Hetian Characteristic Chinese Traditional Medicine Research, Xinjiang Hetian College, Hetian, 848099, PR China. FAU - Wang, Ju-Tao AU - Wang JT AD - School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, PR China; Anhui Province Key Laboratory of Bioactive Natural Products, Hefei, 230012, PR China. Electronic address: wjt591@ahtcm.edu.cn. FAU - Wang, Guo-Kai AU - Wang GK AD - School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, PR China; Anhui Province Key Laboratory of Bioactive Natural Products, Hefei, 230012, PR China. Electronic address: wanggk@ahtcm.edu.cn. FAU - Yu, Yang AU - Yu Y AD - School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, PR China; Anhui Province Key Laboratory of Bioactive Natural Products, Hefei, 230012, PR China; Functional Activity and Resource Utilization on Edible and Medicinal Fungi Joint Laboratory of Anhui Province, Hefei, 230012, PR China. Electronic address: yyang71@ahtcm.edu.cn. LA - eng PT - Journal Article DEP - 20261025 PL - United States TA - Bioorg Chem JT - Bioorganic chemistry JID - 1303703 SB - IM OTO - NOTNLM OT - Anti-melanogenic activity OT - Crocus sativus OT - Cyclogeraniol OT - Enantiomerism OT - MITF/TYR OT - Saffron OT - Tyrosinase COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:13 PHST- 2026/06/29 00:00 [received] PHST- 2026/10/20 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:13 [entrez] AID - S0045-2068(26)01232-0 [pii] AID - 10.1016/j.bioorg.2026.110696 [doi] PST - aheadofprint SO - Bioorg Chem. 2026 Oct 25;184:110696. doi: 10.1016/j.bioorg.2026.110696. PMID- 42950793 OWN - NLM STAT- Publisher LR - 20261028 IS - 1090-2120 (Electronic) IS - 0045-2068 (Linking) VI - 184 DP - 2026 Oct 23 TI - Discovery of novel pyridine-2-tetrazole derivatives as in vivo memory enhancement agents. PG - 110683 LID - S0045-2068(26)01219-8 [pii] LID - 10.1016/j.bioorg.2026.110683 [doi] AB - Despite the currently incurable nature of Alzheimer's disease (AD), acetylcholinesterase (AChE) inhibitors remain the primary therapeutic strategy for symptom management. In this study, twenty-five novel pyridine-2-tetrazole derivatives were designed, their binding affinities with AChE were predicted using machine learning models, and their drug-likeness were calculated. Following their synthesis, the compounds were evaluated in vitro for AChE inhibitory activity. Based on these findings, lead compounds 11 and 28 were selected for in vivo assessment to determine their effects on cognitive function in trimethyltin (TMT)-induced cognitive impairment mouse models at dosages of 20 mg/kg and 60 mg/kg. Although compound 28 exhibited modest in vitro AChE inhibition, it significantly improved cognitive performance in TMT-induced mice across both dosage groups. This cognitive enhancement was demonstrated through the Object Recognition Test, modified Y-maze, and Passive Avoidance Test. These findings suggest that compound 28 may exert its neuroprotective effects through a different molecular mechanism rather than exclusive AChE inhibition, highlighting its potential as a lead compound for the treatment of cognitive impairment and related neurodegenerative disorders such as AD. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Hung, Nguyen Viet AU - Hung NV AD - Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Cua Nam, Hanoi, 11021, Viet Nam; Hanoi University of Mining and Geology, 18 Vien, Bac Tu Liem, Hanoi, 11910, Viet Nam. FAU - Hoang, Van-Hai AU - Hoang VH AD - Faculty of Pharmacy, PHENIKAA University, Hanoi, 12116, Viet Nam. FAU - Long, Le Thien Bao AU - Long LTB AD - Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Cua Nam, Hanoi, 11021, Viet Nam. FAU - Linh, Vu Ngoc Hai AU - Linh VNH AD - Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Cua Nam, Hanoi, 11021, Viet Nam. FAU - Tien, Le Quoc AU - Tien LQ AD - Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Cua Nam, Hanoi, 11021, Viet Nam. FAU - Linh, Tran Vu Thuy AU - Linh TVT AD - Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Cua Nam, Hanoi, 11021, Viet Nam. FAU - Bang, Le Thi Tam AU - Bang LTT AD - Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Cua Nam, Hanoi, 11021, Viet Nam. FAU - Nguyen, Tiep K AU - Nguyen TK AD - Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Cua Nam, Hanoi, 11021, Viet Nam. FAU - Pham, Hang Thi Nguyet AU - Pham HTN AD - Faculty of Pharmacy, Phenikaa School of Medicine and Pharmacy, Phenikaa University, Nguyen Trac, Duong Noi, Hanoi, 12114, Viet Nam. FAU - Minh, Dinh Thi AU - Minh DT AD - National Institute of Medicinal Materials, Cua Nam, Hanoi, 100000, Viet Nam. FAU - Hien, Tran Thi Thu AU - Hien TTT AD - Vietnam University of Traditional Medicine, 2 Tran Phu, Ha Dong, Hanoi, 100000, Viet Nam. FAU - Wang, Shuai AU - Wang S AD - Department of Chemistry, Engineering Center of Catalysis and Synthesis for Chiral Molecules, Shanghai Engineering Research Center of Industrial Asymmetric Catalysis of Chiral Drugs, State Key Laboratory of Green Chemical Synthesis and Conversion, Fudan University, Shanghai, China. FAU - Tran, Phuong-Thao AU - Tran PT AD - Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Cua Nam, Hanoi, 11021, Viet Nam. Electronic address: thaotp@hup.edu.vn. LA - eng PT - Journal Article DEP - 20261023 PL - United States TA - Bioorg Chem JT - Bioorganic chemistry JID - 1303703 SB - IM OTO - NOTNLM OT - AChE inhibitors OT - Alzheimer's disease OT - In vivo OT - Machine learning OT - Pyridine OT - TMT-induced OT - Tetrazole COIS- Declaration of coCmpeting interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:13 PHST- 2026/08/01 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:13 [entrez] AID - S0045-2068(26)01219-8 [pii] AID - 10.1016/j.bioorg.2026.110683 [doi] PST - aheadofprint SO - Bioorg Chem. 2026 Oct 23;184:110683. doi: 10.1016/j.bioorg.2026.110683. PMID- 42950787 OWN - NLM STAT- Publisher LR - 20261028 IS - 1532-1991 (Electronic) IS - 0143-4160 (Linking) VI - 138 DP - 2026 Oct 22 TI - Glutathione peroxidase 8 overexpression promotes invasion and metastasis via the store-operated calcium entry signaling axis in poorly differentiated lung adenocarcinoma. PG - 103207 LID - S0143-4160(26)00100-4 [pii] LID - 10.1016/j.ceca.2026.103207 [doi] AB - Poorly differentiated lung adenocarcinoma (PDLA) is the most aggressive subtype and characterized by early metastasis, rapid progression, and a dismal prognosis. Glutathione peroxidase 8 (GPX8) is frequently upregulated in poorly differentiated tumors and closely associated with poor survival, yet its specific role in the invasion and metastasis of PDLA remains poorly defined. Emerging evidence implicates GPX8 in calcium (Ca(2)(+)) homeostasis; therefore, we investigated whether GPX8 promotes the invasion and metastasis of PDLA by regulating Ca(2)(+) signaling. Bioinformatics analysis, immunohistochemistry (IHC), western blotting (WB), and next-generation sequencing (NGS) consistently confirmed high expression levels of GPX8 in PDLA tissues and NCI-H1975 cell lines. The overexpression of GPX8 depleted Ca(2)(+) stores in the endoplasmic reticulum (ER) and enhanced Ca(2)(+) influx in a manner that was partially dependent on Orai1-mediated store-operated calcium entry (SOCE) and could be attenuated by CM 4620 (dual Orai1/2 channel inhibitor)and knockdown of Orai1. The effect of SOCE induced by thapsigargin (TG) was analogous to the phenomenon observed upon GPX8 overexpression, and this classical SOCE-mediated Ca(2)(+) influx was almost completely abrogated by treatment with 20 muM of CM4620 (P < 0.01). Moreover, GPX8 downregulated E-cadherin, upregulated Vimentin, promoted invasion in vitro, and induced lung metastasis in nude mice. Interestingly, siRNA-mediated Orai1 knockdown elevated E-cadherin expression, reduced Vimentin levels, and suppressed epithelial-mesenchymal transition (EMT). Bioinformatics prediction and dual-luciferase reporter assays verified that the Ca(2)(+)-dependent transcription factors CREB1 and CREB3 positively regulated GPX8 transcription, forming a potential feed-forward loop to amplify SOCE signaling. Our findings reveal that GPX8 acts as a critical driver of PDLA invasion and metastasis via CREB1/3-regulated SOCE-mediated Ca(2)(+) signaling and epithelial-mesenchymal transition (EMT), suggesting that targeting the GPX8/SOCE/Ca(2)(+) axis may represent a promising therapeutic strategy for PDLA. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Liang, Yun AU - Liang Y AD - Department of Cardiothoracic Surgery, The Third Affiliated Hospital of Soochow University, Changzhou, 213000, PR China; Department of Thoracic Surgery, Yantaishan Hospital Affiliated to Shandong Medical and Pharmaceutical University, Yantai, 264000, PR China. FAU - Yang, Menghao AU - Yang M AD - Department of Biochemistry and Molecular Biology, Shandong Medical and Pharmaceutical University, Yantai, 264000, PR China. FAU - Wang, Bin AU - Wang B AD - Department of Cardiothoracic Surgery, The Third Affiliated Hospital of Soochow University, Changzhou, 213000, PR China. FAU - Ren, Mengmeng AU - Ren M AD - Department of Cardiology, Yuhuangding Hospital Affiliated to Qingdao University, Yantai, 264000, PR China. FAU - Liu, Ying AU - Liu Y AD - Department of Pathology, Yantaishan Hospital Affiliated to Shandong Medical and Pharmaceutical University, Yantai, 264000, PR China. FAU - Li, Qiang AU - Li Q AD - Department of Pathology, Yantaishan Hospital Affiliated to Shandong Medical and Pharmaceutical University, Yantai, 264000, PR China. FAU - Wang, Runze AU - Wang R AD - Department of Biochemistry and Molecular Biology, Shandong Medical and Pharmaceutical University, Yantai, 264000, PR China. FAU - Lv, Guohui AU - Lv G AD - Department of Biochemistry and Molecular Biology, Shandong Medical and Pharmaceutical University, Yantai, 264000, PR China. FAU - Li, Youjie AU - Li Y AD - Department of Biochemistry and Molecular Biology, Shandong Medical and Pharmaceutical University, Yantai, 264000, PR China. FAU - Liu, Shuliang AU - Liu S AD - Department of Thoracic Surgery, Yantaishan Hospital Affiliated to Shandong Medical and Pharmaceutical University, Yantai, 264000, PR China. FAU - Mu, Xuri AU - Mu X AD - Department of Thoracic Surgery, Yantaishan Hospital Affiliated to Shandong Medical and Pharmaceutical University, Yantai, 264000, PR China. FAU - Zhang, Wei AU - Zhang W AD - Department of Pathology, Yantaishan Hospital Affiliated to Shandong Medical and Pharmaceutical University, Yantai, 264000, PR China. FAU - Xie, Ning AU - Xie N AD - Department of Thoracic Surgery, Yantaishan Hospital Affiliated to Shandong Medical and Pharmaceutical University, Yantai, 264000, PR China. Electronic address: sunnyvvv@163.com. FAU - Di, Dongmei AU - Di D AD - Department of Cardiothoracic Surgery, The Third Affiliated Hospital of Soochow University, Changzhou, 213000, PR China. Electronic address: ddm5122@163.com. LA - eng PT - Journal Article DEP - 20261022 PL - Netherlands TA - Cell Calcium JT - Cell calcium JID - 8006226 SB - IM OTO - NOTNLM OT - Adenocarcinoma OT - Calcium OT - Glutathione peroxidase 8 (GPX8) OT - Lung cancer OT - Store-operated calcium entry (SOCE) COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:13 PHST- 2026/06/18 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:13 [entrez] AID - S0143-4160(26)00100-4 [pii] AID - 10.1016/j.ceca.2026.103207 [doi] PST - aheadofprint SO - Cell Calcium. 2026 Oct 22;138:103207. doi: 10.1016/j.ceca.2026.103207. PMID- 42950785 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-4138 (Electronic) IS - 0309-1740 (Linking) VI - 243 DP - 2026 Oct 16 TI - Comprehensive assessment of prediction of pH in red meat based on near-infrared spectroscopy (NIRS). PG - 110240 LID - S0309-1740(26)00213-5 [pii] LID - 10.1016/j.meatsci.2026.110240 [doi] AB - pH is an indicator of meat quality, affecting colour, tenderness, and shelf life. This study is aimed to carry a comprehensive assessment of non-invasive prediction model for pH using Near-Infrared Spectroscopy (NIRS) across red meat species. A calibration model based on 2539 samples was built using a deep convolutional neural network (DCNN), integrating data from 17 muscles, 3 species (beef, lamb, venison), and 4 NIRS devices. The model achieved a strong calibration performance (R(2) = 0.95, SEP = 0.07) and a competent validation performance (R(2) = 0.82, SEP = 0.14). External testing confirmed the model's ability to predict pH across different animals and muscles, although reduced accuracy was observed with the INDIGO handheld device. The lack of ability to capture information related to muscle structure reduced the prediction performance of the sensor and generated bias. Results confirm the feasibility of a robust NIRS-based tool for meat pH assessment, provided device used to collect NIR spectra is suitable for red meat. This approach opens new opportunities for real-time quality control in meat processing, with a potential to improve consistency and reduce losses due to pH-related defects. CI - Copyright (c) 2026 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Ellies-Oury, M P AU - Ellies-Oury MP AD - Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (INRAE), Universite Clermont Auvergne, VetAgro Sup, UMR1213, Recherches sur les Herbivores, Theix, Saint-Genes Champanelle, 63122, France; Bordeaux Sciences Agro, CS 40201, Gradignan, 33175, France; Bioeconomy Science Institute, Palmerston North, New Zealand. Electronic address: marie-pierre.ellies@agro-bordeaux.fr. FAU - Realini, C E AU - Realini CE AD - Bioeconomy Science Institute, Palmerston North, New Zealand. FAU - Zhang, R AU - Zhang R AD - Bioeconomy Science Institute, Palmerston North, New Zealand. FAU - Dixit, Y AU - Dixit Y AD - Bioeconomy Science Institute, Palmerston North, New Zealand. FAU - Reis, M M AU - Reis MM AD - Bioeconomy Science Institute, Palmerston North, New Zealand. Electronic address: marlon.m.reis@agresearch.co.nz. LA - eng PT - Journal Article DEP - 20261016 PL - England TA - Meat Sci JT - Meat science JID - 101160862 SB - IM OTO - NOTNLM OT - Near-infrared spectroscopy OT - Non-invasive prediction OT - Red meat OT - Spectral analysis OT - pH COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:12 PHST- 2025/09/04 00:00 [received] PHST- 2026/08/25 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:12 [entrez] AID - S0309-1740(26)00213-5 [pii] AID - 10.1016/j.meatsci.2026.110240 [doi] PST - aheadofprint SO - Meat Sci. 2026 Oct 16;243:110240. doi: 10.1016/j.meatsci.2026.110240. PMID- 42950772 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-3320 (Electronic) IS - 0960-7404 (Linking) VI - 69 DP - 2026 Oct 26 TI - International survey on neoadjuvant treatments in anatomically resectable pancreatic adenocarcinoma (INSPIRA): a real-life snapshot. PG - 102580 LID - S0960-7404(26)00231-8 [pii] LID - 10.1016/j.suronc.2026.102580 [doi] AB - BACKGROUND: Neoadjuvant therapy (NAT) has shown promising results in borderline and locally advanced pancreatic ductal adenocarcinoma (PDAC). Together with the concept of biological resectability, this has raised interest in extending NAT to anatomically resectable PDAC (AR-PDAC). METHODS: An international online survey was conducted among surgeons, oncologists, radiotherapists, and gastroenterologists to assess current perspectives on NAT in AR-PDAC. RESULTS: A total of 391 responses from 61 countries were collected. Overall, 77% reported discussing all pancreatic cancer cases in multidisciplinary meetings. NAT was recommended only for selected AR-PDAC patients by 47% of respondents. The main selection criteria were elevated CA19-9 (74.6%), suspicious regional lymph nodes (63.4%), and suspected occult micro-metastases (55.6%). Disease progression during therapy, potentially precluding surgery, was a concern for 26.5%. However, 93.3% agreed that NAT can help assess tumor biology and avoid futile surgery in rapidly progressing disease. Regarding future practice, 53.6% anticipated NAT becoming standard care for all AR-PDAC, while 34.6% supported its use only in selected cases. CONCLUSION: NAT for AR-PDAC attracts interest among surveyed clinicians worldwide. Although currently reserved mainly for selected patients, its potential to refine patient selection, avoid futile surgery, and support more aggressive surgical strategies in responders may encourage evaluation and adoption. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - D'Alterio, Chiara AU - D'Alterio C AD - Department of Surgery, Umberto I University Hospital, Sapienza University of Rome, Rome, Italy. FAU - Lai, Quirino AU - Lai Q AD - General Surgery and Organ Transplantation Unit, Umberto I University Hospital, Sapienza University of Rome, Rome, Italy. FAU - Lanzone, Maria AU - Lanzone M AD - Department of Surgery, Umberto I University Hospital, Sapienza University of Rome, Rome, Italy. FAU - Valente, Roberto AU - Valente R AD - Department of Diagnostic and Intervention, Surgery, Umea University, Umea, Sweden. FAU - da Correggio, Matteo Fabbrici AU - da Correggio MF AD - Department of Surgery, Umberto I University Hospital, Sapienza University of Rome, Rome, Italy. FAU - Iannone, Immacolata AU - Iannone I AD - Department of Surgery, Umberto I University Hospital, Sapienza University of Rome, Rome, Italy. FAU - Coppola, Alessandro AU - Coppola A AD - Department of Surgery, Umberto I University Hospital, Sapienza University of Rome, Rome, Italy. Electronic address: aless.coppola@uniroma1.it. CN - INSPIRA Collaborative Group LA - eng PT - Journal Article DEP - 20261026 PL - Netherlands TA - Surg Oncol JT - Surgical oncology JID - 9208188 SB - IM COIS- Conflicts of interest Dr. Valente is consultant for Boston Scientific. The authors declare that there is no conflict of interest regarding the publication of this article. FIR - Berkane, Salah IR - Berkane S IRAD- Faculty of medecine Algiers. University of Algiers. EPH IBN ZIRI Bologhine, Algiers, Algeria. Electronic address: salahberkane07@yahoo.fr. FIR - Koltai, Tomas IR - Koltai T IRAD- The Hospital Centro Gallego of Buenos Aires, Argentina. Electronic address: tomaskoltai@gmail.com. FIR - McCormack, Lucas IR - McCormack L IRAD- Hospital Aleman of Buenos Aires, Argentina. Electronic address: lmccormack@hospitalaleman.com. FIR - Barreto, S George IR - Barreto SG IRAD- Flinders University, Australia. Electronic address: georgebarreto@yahoo.com. FIR - O'Rourke, Nick IR - O'Rourke N IRAD- Royal Brisbane Hospital, Australia. Electronic address: Orourke.nick@gmail.com. FIR - Muralidhaan, Vijayaragavan IR - Muralidhaan V IRAD- University of Melbourne Department of Surgery, Austin Health, Australia. Electronic address: muv@unimelb.edu.au. FIR - Oo, June IR - Oo J IRAD- Alfred Health, Australia. Electronic address: oojune@gmail.com. FIR - Perini, Marcos Vinicius IR - Perini MV IRAD- Surgery at the University of Melbourne, Australia. Electronic address: marcos.perini@unimelb.edu.au. FIR - Gananadha, Sivakumar IR - Gananadha S IRAD- Canberra Hospital, Australia. Electronic address: siva@gananadha.com.au. FIR - Luo, Yuchen IR - Luo Y IRAD- Northern Health, Australia. Electronic address: Yuchen.Frank.Luo@gmail.com. FIR - Orourke, Tom IR - Orourke T IRAD- Greenslopes Private Hospital and the Mater Hospital, Australia. Electronic address: thomas.orourke@health.wld.gov.au. FIR - Klug, Reinhold IR - Klug R IRAD- Horn-Allentsteig State Hospital, Australia. Electronic address: reinhold.klug@horn.lknoe.at. FIR - Kirchweger, Patrick IR - Kirchweger P IRAD- Department of Surgery, Ordensklinikum Linz, Austria. Electronic address: patrickkirchweger@gmx.at. FIR - Strobel, Oliver IR - Strobel O IRAD- Medical University of Vienna, Austria. Electronic address: oliver.strobel@meduniwien.ac.at. FIR - Bayramov, Nuru IR - Bayramov N IRAD- Azerbaijan Medical University, Azerbaijan. Electronic address: nurubay2006@yahoo.com. FIR - Abreu de Carvalho, Luis Filipe IR - Abreu de Carvalho LF IRAD- Ghent University Hospital, Belgium. Electronic address: luis.abreudecarvalho@ugent.be. FIR - Frederik, Berrevoet IR - Frederik B IRAD- Ghent University Hospital, Belgium. Electronic address: frederik.berrevoet@ugent.be. FIR - Navez, Julie IR - Navez J IRAD- Departement of Abdominal Surgery and Transplantation, Belgium. Electronic address: julie.navez@hubruxelles.be. FIR - Apers, Thomas IR - Apers T IRAD- Ziekenhuis aan de stroom (ZAS) - Antwerp - Belgium, Belgium. Electronic address: Thomas.apers@zas.be. FIR - Vera, Hartman IR - Vera H IRAD- University of Antwerp, Belgium. Electronic address: vera.hartman@uza.be. FIR - Lancelot, Marique IR - Lancelot M IRAD- Cliniques Ubiversitaires, Belgium. Electronic address: lancelot.c.marique@gmail.com. FIR - Roeyen, Geert IR - Roeyen G IRAD- University Hospital Antwerp, Belgium. Electronic address: geert.roeyen@uza.be. FIR - Osvaldt, Alessandro IR - Osvaldt A IRAD- Universidade Federal do Rio Grande do Sul, Brazil. Electronic address: aosvaldt@hcpa.edu.br. FIR - Araruna, Gustavo IR - Araruna G IRAD- Hiae, United Arab Emirates. Electronic address: gustavoararuna@hotmail.com. FIR - Utrini, Murillo IR - Utrini M IRAD- State University of Campinas - UNICAMP, Brazil. Electronic address: mutrini@gmail.com. FIR - Dardnov, Dragomir IR - Dardnov D IRAD- University Hospital Lozentez, Sofia, Bulgaria. Electronic address: drdardanov@abv.bg. FIR - Bennett, Sean IR - Bennett S IRAD- Queen's University, Canada. Electronic address: sean.bennett@kingstonhsc.ca. FIR - Rousek, Michael IR - Rousek M IRAD- 2nd Faculty of Medicine, Charles University in Prague and Military University Hospital in Prague, Czech Republic. Electronic address: rousek.uvn@gmail.com. FIR - Branes, Alejandro IR - Branes A IRAD- Complejo Asistencial Dr. Sotero del Rio, Chile. Electronic address: alejandro.branes@sotero.cl. FIR - Martinez Castillo, Jorge IR - Martinez Castillo J IRAD- Surgery School of Medicine. Pontificia Universidad Catolica de Chile, Chile. Electronic address: jamartin@med.puc.cl. FIR - Figueroa, Eduardo IR - Figueroa E FIR - Liang, Tingbo IR - Liang T IRAD- The First Affiliated Hospital of Zhejiang University School of Medicine, China. Electronic address: liangtingbo@zju.du.cn. FIR - Jiang, Li IR - Jiang L IRAD- Huazhong University of Science and Technology, Tongji medical college, Tongji hospital, China. Electronic address: jiangli@tjh.tjmu.edu.cn. FIR - Lu, Zipeng IR - Lu Z IRAD- The First Affiliated Hospital with Nanjing Medical University, China. Electronic address: surgeonmark@hotmail.com. FIR - Duarte, Alvaro IR - Duarte A IRAD- AHPBA-IHPBA, United States. Electronic address: alvaro.andres.duarte@gmail.com. FIR - Oliverius, Martin IR - Oliverius M IRAD- Surgery Clinic 3.Medical Faculty and Royal Vinohrady Hospital Prague, Czech Republic. Electronic address: martin.oliverius@fnkv.cz. FIR - Molacek, Jiri IR - Molacek J IRAD- University Hospital in Pilsen, Czech Republic. Electronic address: molacek@fnplzen.cz. FIR - Tschuor, Christoph IR - Tschuor C IRAD- Department of Transplantation, Digestive Disease and General Surgery Center of Cancer and Transplantation Rigshospitalet, Copenhagen University Hospital, Inge Lehmannsvej 7, Copenhagen O, DK- 2100, Denmark. Electronic address: christoph.tschuor@regionh.dk. FIR - Aspiazu, Cristhian IR - Aspiazu C IRAD- Hospital Teodoro Maldonado Carbo, Ecuador. Electronic address: cristhian_aspiazu@hotmail.com. FIR - Lucia, Marta Cueva IR - Lucia MC IRAD- AHPBA, United States. Electronic address: martacueva27@gmail.com. FIR - Barzallo, David IR - Barzallo D IRAD- AHPBA IHPBA COMBINED, United States. Electronic address: drdavidbarzallo@gmail.com. FIR - Finch-Jones, Margaret IR - Finch-Jones M IRAD- University Hospitals Bristol and Weston NHS Foundation Trust, United Kingdom. Electronic address: meg.finch-jones@uhbw.nhs.uk. FIR - Mekonnen, Nebiat Embiale IR - Mekonnen NE IRAD- HPB surgery, United Kingdom. Electronic address: nebiatbdu@gmail.com. FIR - Bezabih, Yoseph Solomon IR - Bezabih YS IRAD- Haramaya University, Ethiopia. Electronic address: josephsol87@gmail.com. FIR - Kokkola, Arto IR - Kokkola A IRAD- Helsinki University Hospital, Finland. Electronic address: arto.kokkola@hus.fi. FIR - Gabriella, Pittau IR - Gabriella P IRAD- Centre Hepatobiliaire Paul Brousse, France. Electronic address: gabriella.pittau@gmail.com. FIR - Daniel, Azoulay IR - Daniel A IRAD- CHB, France. Electronic address: daniel.azoulay@aphp.fr. FIR - Antonio, Sa Cunha IR - Antonio SC IRAD- Centre hepatobilaire Hopital Paul Brousse, France. Electronic address: Antonio.sacunha@aphp.com. FIR - Addeo, Pietro IR - Addeo P IRAD- University of Strasbourg, France. Electronic address: pietrofrancesco.addeo@chru-strasbourg.fr. FIR - Marchese, Ugo IR - Marchese U IRAD- Department of Digestive, HPB and Endocrine Surgery, Hopital Cochin, AP-HP Centre, 27 rue du Faubourg St Jacques, Paris, 75014, France; Universite de Paris Cite, 15 rue de l'ecole de medecine, Paris, 75006, France. Electronic address: umarchese1@gmail.com. FIR - Rosso, Edoardo IR - Rosso E IRAD- Pole Sante Sud, Le Mans, France. Electronic address: edoardo_rosso@hotmail.com. FIR - Florence, Huguet IR - Florence H IRAD- Tenon Hospital, France. Electronic address: florence.huguet@aphp.fr. FIR - Safi, Dokmak IR - Safi D IRAD- HPB surgery and liver transplantation, Beaujon hospital, Clichy, France. Electronic address: safi.dokmak@aphp.fr. FIR - Alexandre, Doussot IR - Alexandre D IRAD- CHU Besancon, France. Electronic address: lex.doussot@yahoo.com. FIR - Laurence, Chiche IR - Laurence C IRAD- CHU BORDEAUX, France. Electronic address: laurence.chiche@chu-bordeaux.fr. FIR - Gumbs, Andrew IR - Gumbs A IRAD- Hopital Antoine Beclere, Assistance Publique-Hopitaux de Paris, France. Electronic address: andrew.gumbs@aphp.fr. FIR - De Rosa, Raffaele Vincenzo IR - De Rosa RV IRAD- CHU Rennes, France. Electronic address: Raffaelevincenzo.de.rosa@chu-rennes.fr. FIR - Micelli Lupinacci, Renato IR - Micelli Lupinacci R IRAD- Hopital Ambroise-Pare. APHP Universite Paris Saclay, France. Electronic address: rmlupinacci@gmail.com. FIR - Epis, Lorenzo IR - Epis L IRAD- Hopital de hautepierre, France. Electronic address: loreepis@gmail.com. FIR - Ronellenfitsch, Ulrich IR - Ronellenfitsch U IRAD- University Hospital Halle (Saale), Germany. Electronic address: ulrich.ronellenfitsch@uk-halle.de. FIR - Kleeff, Jorg IR - Kleeff J IRAD- Martin-Luther-University Halle-Wittenberg, Germany. Electronic address: joerg.kleeff@uk-halle.de. FIR - Stavrou, Gregor A IR - Stavrou GA IRAD- Klinikum Saarbruecken, Germany. Electronic address: gstavrou@klinikum-saarbruecken.de. FIR - Wagner, Kim Caroline IR - Wagner KC IRAD- Asklepios Klinik Barmbek, Germany. Electronic address: ki.wagner@asklepios.com. FIR - Felix, Nickel IR - Felix N IRAD- University Medical Center Hamburg-Eppendorf, Germany. Electronic address: f.nickel@uke.de. FIR - Ruess, Dietrich A IR - Ruess DA IRAD- General and Visceral Surgery - Medical Center University of Freiburg - Germany, Germany. Electronic address: dietrich.ruess@uniklinik-freiburg.de. FIR - Faik Guntac, Uzunoglu IR - Faik Guntac U IRAD- University Hospital Hamburg-Eppendorf, Germany. Electronic address: f.uzunoglu@uke.de. FIR - Rebelo, Artur IR - Rebelo A IRAD- Uniklinikum Halle (Aale), Germany. Electronic address: artur.rebelo@uk-halle.de. FIR - Ntourakis, Dimitrios IR - Ntourakis D IRAD- Division of Surgery, School of Medicine, European University Cyprus, Germany. Electronic address: dntourakis@hotmail.com. FIR - Schizas, Dimitrios IR - Schizas D IRAD- FIRST DEPARTMENT OF SURGERY, NATIONAL AND KAPODISTRIAN UNIVERSITY OF ATHENS, LAIKON GENERAL HOSPITAL, ATHENS, Greece. Electronic address: schizasad@gmail.com. FIR - Karydakis, Lysandros IR - Karydakis L IRAD- First Department of surgery,"Laiko" General Hospital, NKUOA, Greece. Electronic address: lys_karydakis@yahoo.gr. FIR - Syllaios, Athanasios IR - Syllaios A IRAD- Laiko University Hospital, Greece. Electronic address: nh_reas@hotmail.com. FIR - Vailas, Michail IR - Vailas M IRAD- Laiko General Hospital, Greece. Electronic address: mike_vailas@yahoo.com. FIR - Katsaros, Ioannis IR - Katsaros I IRAD- First Department of Surgery, National and Kapodistrian University of Athens, Laikon General Hospital, Greece. Electronic address: gikats.md@gmail.com. FIR - Tsoulfas, Georgios IR - Tsoulfas G IRAD- Aristotle University School of Medicine, Greece. Electronic address: tsoulfasg@gmail.com. FIR - Kapiris, Stylianos IR - Kapiris S IRAD- EVANGELISMOS General Hospital of Athens, Greece. Electronic address: stkapiris@hotmail.com. FIR - Tsaramanidis, Savvas IR - Tsaramanidis S IRAD- General Surgeon, Interbalkan Medical Center, Greece. Electronic address: tsarasavvas@gmail.com. FIR - Papadakos, Stavros IR - Papadakos S IRAD- First Department of Gastroenterology, United States. Electronic address: stavrospapadakos@gmail.com. FIR - Sotiropoulou, Maria IR - Sotiropoulou M IRAD- Evaggelismos General hospital Athens greece, Greece. Electronic address: marosotiropoulou@gmail.com. FIR - Panagakis, Andreas IR - Panagakis A IRAD- Laiko General Hospital of Athens, Aristotle University of Thessaloniki (AUTH), Greece. Electronic address: andrew19921908@gmail.com. FIR - Antonakis, Pantelis IR - Antonakis P IRAD- 2nd Dept of Surgery, NKUA, Aretaieion Hospital, Athens, Greece. Electronic address: pantonakis@med.uoa.gr. FIR - Triantafyllidis, Ioannis IR - Triantafyllidis I IRAD- General Hospital of Kavala, Greece. Electronic address: jtrianta@gmail.com. FIR - Kofina, Konstantinia IR - Kofina K IRAD- Genesis Clinic, Greece. Electronic address: NADIAKOFINA@YAHOO.COM. FIR - Machairas, Nikolaos IR - Machairas N IRAD- 2nd Department of Propaeudeutic Surgery, NKUA, Greece. Electronic address: nmachair@gmail.com. FIR - Mylonakis, Adam IR - Mylonakis A IRAD- First Department of Surgery, Laikon General Hospital, National and Kapodistrian University of Athens, Athens, Greece. Electronic address: adam.mylonakis@gmail.com. FIR - Toutouzas, Konstantinos IR - Toutouzas K IRAD- NATIONAL AND KAPODISTRIAN UNIVERSITY OF ATHENS, Greece. Electronic address: tousur@hotmail.com. FIR - Garmpis, Nikolaos IR - Garmpis N IRAD- Surgical Department, Sotiria General Hospital, Athens, Greece. Electronic address: nikosg22@hotmail.com. FIR - Damaskos, Christos IR - Damaskos C IRAD- N. S. Christeas Laboratory of Experimental Surgery and Surgical Research, Medical School, National and Kapodistrian University of Athens, Athens, Greece. Electronic address: x_damaskos@yahoo.gr. FIR - Efremidou, Eleni IR - Efremidou E IRAD- First Surgical Department, University Hospital of Alexandroupolis, Democritus University of Thrace, Alexandroupolis, Greece. Electronic address: eeffraem@med.duth.gr. FIR - Tzimas, Georgios IR - Tzimas G IRAD- Hygeia Mitera Hospital, Greece. Electronic address: GEORGE_TZIMAS@HOTMAIL.COM. FIR - Angelakoudis, Apostolos IR - Angelakoudis A FIR - Sakarellos, Panagiotis IR - Sakarellos P IRAD- G.N.A. LAIKO, ATHENS, Greece. Electronic address: panagiotissakarellos@gmail.com. FIR - Contreras, Jose IR - Contreras J FIR - Marimuthu, Raghunath Raja IR - Marimuthu RR IRAD- IMS and SUM Hospital, India. Electronic address: raghunath89@gmail.com. FIR - Vasavada, Bhavin IR - Vasavada B FIR - Sundaram, Sridhar IR - Sundaram S IRAD- Tata Memorial Hospital, Mumbai, India. Electronic address: drsridharsundaram@gmail.com. FIR - Gupta, Amit IR - Gupta A IRAD- AIIMS Rishikesh, India. Electronic address: dramit2411@gmail.com. FIR - Gupta, Sweety IR - Gupta S IRAD- AIIMS Rishikesh, India. Electronic address: drsg2411@gmail.com. FIR - Gupta, Archit IR - Gupta A IRAD- Department of GI Surgery, Medanta the Medicity, Gurugram, India. Electronic address: archit9th@gmail.com. FIR - Bhaduri, Debanshu IR - Bhaduri D IRAD- M N Budhrani Cancer Institute, SVMMC, Pune, Maharashtra, India. Electronic address: radical.surgeon@gmail.com. FIR - Bora, Dibyajyoti IR - Bora D IRAD- Apollo Hospitals Guwahati, G.S. Road, Christian Basti, Guwahati, India. Electronic address: dibyarashmi@yahoo.com. FIR - Parikh, Kush IR - Parikh K IRAD- Sanjay Gandhi Post graduate Institute of Medical Sciences, India. Electronic address: parikhkush28@gmail.com. FIR - O'Connell, Robert Michael IR - O'Connell RM IRAD- St Vincent's University Hospital, Dublin, Ireland. Electronic address: robertmoconnell@rcsi.com. FIR - Mahamid, Ahmad IR - Mahamid A IRAD- Carmel Medical Center, Haifa, Israel. Electronic address: Mahamidam@yahoo.com. FIR - Dagan, Amir IR - Dagan A IRAD- Shaare Zedek Medical Center, Israel. Electronic address: Adagan@szmc.org.il. FIR - Viti, Virginia IR - Viti V FIR - Farolfi, Tommaso IR - Farolfi T IRAD- Fondazione Policlinico Campus Biomedico di Roma, Italy. Electronic address: t.farolfi@policlinicocampus.it. FIR - Rompianesi, Gianluca IR - Rompianesi G IRAD- Federico II University Hospital of Naples, Italy. Electronic address: gianlucarompianesi@gmail.com. FIR - Carannante, Filippo IR - Carannante F IRAD- UOC Chirurgia Colorettale, Fondazione Policlinico Universitario Campus Bio-Medico di Roma, Italy. Electronic address: f.carannante@policlinicocampus.it. FIR - De Pastena, Matteo IR - De Pastena M IRAD- General and Pancreatic Surgical Department, Italy. Electronic address: matteo.depastena@aovr.veneto.it. FIR - Gronchi, Federico IR - Gronchi F IRAD- AOUI di Verona, sede di Borgo Roma, Chirurgia del pancreas, Italy. Electronic address: gronchifederico@gmail.com. FIR - Salvia, Roberto IR - Salvia R IRAD- University of verona, Italy. Electronic address: roberto.salvia@gmail.com. FIR - Caringi, Silvio IR - Caringi S IRAD- Ospedale Regionale "F. Miulli", Italy. Electronic address: silviocaringi@hotmail.it. FIR - Maurino, Ludovica IR - Maurino L FIR - Romano, Maurizio IR - Romano M IRAD- Chirurgia 2, Clinicizzata Universita di Padova, Ospedale di Treviso, Italy. Electronic address: maurizio.romano@aulss2.veneto.it. FIR - Angrisani, Marco IR - Angrisani M IRAD- Department of General, Hepatobiliary and Pancreatic Surgery, Liver Transplantation Service, San Camillo Forlanini Hospital of Rome, Rome, 00152, Italy. Electronic address: marco.angrisani3@gmail.com. FIR - Pecorelli, Nicolo IR - Pecorelli N IRAD- Division of Pancreatic Surgery, San Raffaele Scientific Institute, Milan, Italy. Electronic address: pecorelli.nicolo@hsr.it. FIR - Kauffmann, Emanuele Federico IR - Kauffmann EF IRAD- UO chirurgia generale e dei trapianti, Pisa, Italy. Electronic address: ekauffmann@hotmail.it. FIR - Carruezzo, Cristina IR - Carruezzo C IRAD- Sapienza - Universita di Roma, Italy. Electronic address: cristina.carruezzo@uniroma1.it. FIR - Luzzi, Andrea-Pierre IR - Luzzi AP IRAD- Universita degli Studi di Genova, Italy. Electronic address: andreapierre.luzzi@gmail.com. FIR - Tersigni, Ilaria IR - Tersigni I IRAD- Ospedale Belcolle/Santa Rosa - Viterbo, Italy. Electronic address: ilariatersigni@yahoo.it. FIR - Leongito, Maddalena IR - Leongito M IRAD- IRCCS pascale, Italy. Electronic address: maddalena.leongito@istitutotumori.na.it. FIR - Casadei, Chiara IR - Casadei C IRAD- General and oncologic surgery unit, Morgagni-Pierantoni Hospital, Ausl Romagna, Forli, Italy. Electronic address: chiara.casadei3@auslromagna.it. FIR - D'Ercole, Gabriele IR - D'Ercole G IRAD- Fondazione Policlinico Universitario Campus Bio-Medico, Italy. Electronic address: g.dercole@policlinicocampus.it. FIR - Di Bucchianico, Alessandro IR - Di Bucchianico A IRAD- Ospedale Santa Maria Della Misericordia Di Urbino, Italy. Electronic address: a.dibucchianico@sanita.marche.it. FIR - Giuliani, Giuseppe IR - Giuliani G IRAD- Misericordia Hospital - department of General and Emergency surgery, Grosseto, Italy. Electronic address: giu.giuliani86@gmail.com. FIR - Tamburrino, Domenico IR - Tamburrino D IRAD- San Raffaele Hospital, Italy. Electronic address: m_tamburrino@hotmail.com. FIR - Attalla El Halabieh, Miriam IR - Attalla El Halabieh M IRAD- Policlinico Agostino Gemelli IRCSS, Italy. Electronic address: Miriam.attalla27@gmail.com. FIR - Coppola, Alessandro IR - Coppola A IRAD- Sapienza University of Rome, Italy. FIR - D'Alterio, Chiara IR - D'Alterio C IRAD- Sapienza University of Rome, Italy. Electronic address: chiara.dalterio@uniroma1.it. FIR - Corallo, Salvatore IR - Corallo S IRAD- Dipartimento di Medicina Interna e Terapia medica, Universita di Pavia, Pavia, Italy. Electronic address: salvatore.corallo@unipv.it. FIR - Biasutto, Dario IR - Biasutto D IRAD- Fondazione Policlinico Universitario Campus Bio-Medico, Italy. Electronic address: dario.biasutto@gmail.com. FIR - Santini, Daniele IR - Santini D IRAD- La Sapienza University of Rome, Italy. Electronic address: daniele.santini@uniroma1.it. FIR - Picone, Vincenzo IR - Picone V IRAD- Policlinico Umberto I, Italy. Electronic address: Vi.picone@policlinicoumberto1.it. FIR - Dell'Aquila, Emanuela IR - Dell'Aquila E IRAD- IRCCS istituto nazionale Tumori regina Elena, Italy. Electronic address: emanuela.dellaquila@gmail.com. FIR - Libia, Annarita IR - Libia A IRAD- Vito Fazzi hospital, Italy. Electronic address: libiamd@me.com. FIR - Giovinazzo, Francesco IR - Giovinazzo F IRAD- San Camillo Hospital, Italy. Electronic address: giovinazzo_francesco@live.com. FIR - Barbarello, Linda IR - Barbarello L IRAD- UO Chirurgia Generale e Trapianti Pisa, Italy. Electronic address: l.barbarello@ao-pisa.toscana.it. FIR - Tufo, Andrea IR - Tufo A IRAD- UOC Chirurgia Generale- Ospedale del mare - Napoli, Italy. Electronic address: tufo.andrea@gmail.com. FIR - Lombardo, Carlo IR - Lombardo C IRAD- Division of General and Transplant Surgery, Italy. Electronic address: carlolombardomd@gmail.com. FIR - Ginesini, Michael IR - Ginesini M IRAD- University of Pisa, Italy. Electronic address: m.ginesini1@gmail.com. FIR - Costa, Francesca IR - Costa F IRAD- Universita di Pisa, Italy. Electronic address: france.83@hotmail.it. FIR - Loiaco, Giuseppe IR - Loiaco G IRAD- Minimally Invasive and Robotic HPB Surgery Division, Federico II University Hospital, Italy. Electronic address: giuseppeloiaco@gmail.com. FIR - Delvecchio, Antonella IR - Delvecchio A IRAD- Ente Ecclesiastico Ospedale Regionale "F. Miulli", Italy. Electronic address: antodel88@libero.it. FIR - Napoli, Niccolo IR - Napoli N IRAD- University of Pisa, Italy. Electronic address: niccolo.napoli@unipi.it. FIR - Morandi, Alessio IR - Morandi A IRAD- HPB Surgery Unit, Department of Experimental and Clinical Medicine, Azienda Ospedaliero Universitaria Careggi, Florence, 50134, Italy. Electronic address: morandialessio9@gmail.com. FIR - Meniconi, Roberto Luca IR - Meniconi RL IRAD- Azienda Ospedaliera San Camillo-Forlanini, Italy. Electronic address: roberto.meniconi@gmail.com. FIR - Turri, Giulia IR - Turri G IRAD- UOC Chirurgia Generale ed Epatobiliare, AOUI Verona, Italy. Electronic address: giulia.turri89@gmail.com. FIR - Di Martino, Marcello IR - Di Martino M IRAD- Universita Del Piemonte Orientale, Italy. Electronic address: marcello.dimartino@uniupo.it. FIR - Scotti, Mauro Alessandro IR - Scotti MA IRAD- IRCCS SAN GERARDO MONZA, Italy. Electronic address: Mauroalessandro.scotti@irccs-sangerardo.it. FIR - Belli, Andrea IR - Belli A IRAD- NATIONAL CANCER INSTITUTE - G. PASCALE - IRCCS, Italy. Electronic address: a.belli@istitutotumori.na.it. FIR - Crippa, Stefano IR - Crippa S IRAD- San Raffaele Hospital Milan & Vita Salute San Raffaele University, Italy. Electronic address: crippa.stefano@hsr.it. FIR - Nicolini, Daniele IR - Nicolini D IRAD- HPB and Abdominal Transplant Surgery, A.O.U. delle Marche, Ancona, Italy. Electronic address: nicolini.daniele1980@gmail.com. FIR - Avella, Pasquale IR - Avella P IRAD- Hepatobiliary and Pancreatic Surgery Unit, Pineta Grande Hospital, Castel Volturno, Caserta, Italy. Electronic address: avella.p@libero.it. FIR - Papis, Davide IR - Papis D IRAD- Ospedale Sant'Anna di Como, Italy. Electronic address: Davide.papis@asst-lariana.it. FIR - Deiro, Giacomo IR - Deiro G IRAD- Department of General Surgery 3, AOU Citta della Salute e della Scienza di Torino "Molinette", Corso Bramante, 88, Turin, 10126, Italy. Electronic address: giacomo.deiro@gmail.com. FIR - Quero, Giuseppe IR - Quero G IRAD- Fondazione Policlinico Agostino Gemelli IRCCS di Roma, Italy. Electronic address: Giuseppe.quero@policlinicogemelli.it. FIR - Gatto, Chiara IR - Gatto C IRAD- Pineta Grande Hospital, Italy. Electronic address: gattochiara974@gmail.com. FIR - Pecora, Irene IR - Pecora I IRAD- Azienda USL Toscana Sud Est, Italy. Electronic address: irene.pecora@gmail.com. FIR - Benedetti Cacciaguerra, Andrea IR - Benedetti Cacciaguerra A IRAD- HPB and Transplant Unit, Polytechnic University of Marche, Italy. Electronic address: dott.benedetti@gmail.com. FIR - Papa, Valerio IR - Papa V IRAD- Digestive Surgery Unit, Fondazione Policlinico Universitario "A. Gemelli" IRCCS, Universita Cattolica del Sacro Cuore, Roma, Italy. Electronic address: valeriopapa@msn.com. FIR - Nappo, Gennaro IR - Nappo G IRAD- Istituto Clinico Humanitas Rozzano, Italy. Electronic address: gennaro.nappo@humanitas.it. FIR - Zingaretti, Caterina Costanza IR - Zingaretti CC IRAD- Azienda Ospedaliera Universitaria Citta della Salute e della Scienza, Torino, Italy. Electronic address: cc.zingaretti@gmail.com. FIR - Spoletini, Gabriele IR - Spoletini G IRAD- Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Italy. Electronic address: gabriele.spoletini@policlinicogemelli.it. FIR - Ripolli, Allegra IR - Ripolli A IRAD- University of Pisa, Italy. Electronic address: allegra.ripolli@gmail.com. FIR - Fiorillo, Claudio IR - Fiorillo C IRAD- Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Italy. Electronic address: claudio.fiorillo@policlinicogemelli.it. FIR - Lancellotti, Francesco IR - Lancellotti F IRAD- Ferrara University Hospital, Italy. Electronic address: md.francesco.lancellotti@gmail.com. FIR - Memeo, Riccardo IR - Memeo R IRAD- Miulli Hospital, Acquaviva delle Fonti (Bari), Italy. Electronic address: r.memeo@miulli.it. FIR - Caputo, Damiano IR - Caputo D IRAD- Fondazione Policlinico Universitario Campus Bio-Medico, Italy. Electronic address: d.caputo@policlinicocampus.it. FIR - Picciariello, Arcangelo IR - Picciariello A IRAD- University of Salento, Lecce, Italy. Electronic address: arcangelopicciariello@gmail.com. FIR - Martines, Gennaro IR - Martines G IRAD- Azienda Ospedaliero Universitaria Policlinico Bari Italy, Italy. Electronic address: gennaromartines@gmail.com. FIR - Nicolazzi, Marco IR - Nicolazzi M IRAD- Universita Del Piemonte Orientale, Italy. Electronic address: Marco.nicolazzimd@gmail.com. FIR - Pacilio, Carlo Alberto IR - Pacilio CA IRAD- Morgagni-Pierantoni Hospital, Forli, Italy. Electronic address: carloalberto.pacilio@gmail.com. FIR - Barberis, Andrea IR - Barberis A IRAD- S.C. Chirurgia Generale ed Epatobiliopancreatica. E.O Ospedali Galliera, Genova, Italy. Electronic address: andrea.barberis@galliera.it. FIR - Giuliani, Alessandra IR - Giuliani A IRAD- Policlinico Riuniti Foggia, Italy. Electronic address: ale.giuliani88@gmail.com. FIR - Tancredi, Marsia IR - Tancredi M IRAD- Universita degli Studi di Torino, Italy. Electronic address: Marsiatancredi.1995@gmail.com. FIR - Tomasicchio, Giovanni IR - Tomasicchio G IRAD- Azienda Ospedaliero Universitaria Policlinico di Bari, Italy. Electronic address: giovannitomasicchio92@gmail.com. FIR - Panzera, Gianmarco IR - Panzera G IRAD- Universita Cattolica del Sacro Cuore di Roma - Universita di Roma la Sapienza, Italy. Electronic address: panzeragianmarco@gmail.com. FIR - Patrono, Damiano IR - Patrono D IRAD- General Surgery 2U- AOU CITTA della Salute e della Scienza di Torino, Italy. Electronic address: damiano.patrono@unito.it. FIR - Morra, Rocco IR - Morra R IRAD- Department of Abdominal Oncology, Experimental Clinical Abdominal Oncology, Istituto Nazionale Tumori di Napoli, IRCCS "G. Pascale", Via M. Semmola, Naples, 80131, Italy. Electronic address: rocco.morra@istitutotumori.na.it. FIR - Scolari, Federica IR - Scolari F IRAD- Azienda ospedaliero universitaria delle marche, Italy. Electronic address: federica.scolari@ospedaliriuniti.marche.ir. FIR - Ingallinella, Sara IR - Ingallinella S IRAD- Hepatobiliary Surgery Division, IRCCS San Raffaele Hospital, Via Olgettina 60, Milan, 20132, Italy. Electronic address: Ingallinella.sara@hsr.it. FIR - Saullo, Paolina IR - Saullo P IRAD- Department of Surgery, Sapienza University of Rome, Viale Regina Elena 324, Rome, 00161, Italy. Electronic address: Paolina.saullo@uniroma1.it. FIR - Tirloni, Luca IR - Tirloni L IRAD- Chirurgia Epato-bilio-pancreatica - Azienda Ospedaliero-Universitaria Careggi, Firenze, Italy. Electronic address: tirloniluca@gmail.com. FIR - Dalla Valle, Raffaele IR - Dalla Valle R IRAD- University of Parma, Italy. Electronic address: raffaele.dallavalle@unipr.it. FIR - Pasquali, Claudio IR - Pasquali C IRAD- Azienda Ospedaliera di Padova, Italy. Electronic address: claudio.pasquali@unipd.it. FIR - Pellegrino, Roberta IR - Pellegrino R IRAD- ARNAS G Brotzu Cagliari, Italy. Electronic address: robipellegrino@gmail.com. FIR - Zurlo, Valeria IR - Zurlo V IRAD- Presidio Ospedaliero Vito Fazzi, Italy. Electronic address: valeriazurlo26@gmail.com. FIR - Coppola, Roberto IR - Coppola R IRAD- Fondazione Policlinico Campus Biomedico, Italy. Electronic address: rbc53rbc@gmail.com. FIR - Saffioti, Maria Cristina IR - Saffioti MC IRAD- La Maddalena Hospital S.P.a, Italy. Electronic address: mariacristina.saffioti@gmail.com. FIR - Manzia, Tommaso Maria IR - Manzia TM IRAD- Tor Vergata University, Italy. Electronic address: manzia@med.uniroma2.it. FIR - Fiore, Michele IR - Fiore M IRAD- Research Unit of Radiation Oncology, Department of Medicine and Surgery, Universita Campus Bio-Medico di Roma, Rome, Italy; Operative Research Unit of Radiation Oncology, Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy. Electronic address: m.fiore@unicampus.it. FIR - Menghi, Roberta IR - Menghi R IRAD- Universita Cattolica del Sacro Cuore - Policlinico Agostino Gemelli Roma, Italy. Electronic address: roberta.menghi@policlinicogemelli.it. FIR - Falconi, Massimo IR - Falconi M IRAD- Pancreatic and Transplant surgery, IRCCS San Raffaele Hospital, Milano, Italy. Electronic address: falconi.massimo@hsr.it. FIR - de Nucci, Germana IR - de Nucci G IRAD- ASST Rhodense presidio di garbagnate milanese, Italy. Electronic address: germanadenucci1@gmail.com. FIR - Binda, Cecilia IR - Binda C IRAD- Gastroenterology and Digestive Endoscopy Unit, Forli-Cesena Hospitals, AUSL Romagna, Italy. Electronic address: cecilia.binda@gmail.com. FIR - Angelico, Roberta IR - Angelico R IRAD- University of Rome Tor Vergata, Italy. Electronic address: Roberta.angelico@gmail.com. FIR - de Luca, Raffaele IR - de Luca R IRAD- Department of Surgical Oncology IRCCS Istituto Tumori " Giovanni Paolo II", Bari, Italy. Electronic address: dr.raffaele.deluca@gmail.com. FIR - Cucchetti, Alessandro IR - Cucchetti A IRAD- Morgagni - Pierantoni Hospital, Italy. Electronic address: alessandro.cucchett2@unibo.it. FIR - Padoan, Virginia IR - Padoan V IRAD- University-Hospital of Padova, Italy. Electronic address: virginia.padoan@aopd.veneto.it. FIR - Brentegani, Giacomo IR - Brentegani G IRAD- General Surgery Mantova, Italy. Electronic address: giacomo.brentegani@asst-mantova.it. FIR - Rapposelli, Ilario Giovanni IR - Rapposelli IG IRAD- Department of Medical Oncology, IRCCS Istituto Romagnolo per lo Studio dei Tumori (IRST) "Dino Amadori", Meldola, Italy. Electronic address: ilario.rapposelli@irst.emr.it. FIR - Arrivi, Giulia IR - Arrivi G IRAD- Oncology Unit Department of Clinical and Molecular Medicine Sapienza University of Rome Sant'Andrea University Hospital Rome Italy, Italy. Electronic address: giulia.arrivi@uniroma1.it. FIR - Marcucci, Stefano IR - Marcucci S IRAD- Ospedale Santa Chiara - Trento, Universita degli Studi di Verona, Italy. Electronic address: stefano.marcucci@apss.tn.it. FIR - Giuliani, Tommaso IR - Giuliani T IRAD- Ospedale Morgagni Pierantoni FOrli, Italy. Electronic address: tommasogiuliani@gmail.com. FIR - Lucchetti, Jessica IR - Lucchetti J IRAD- FONDAZIONE POLICLINICO UNIVERSITARIO CAMPUS BIO MEDICO DI ROMA, via Alvaro del Portillo 200, 00128, Italy. Electronic address: Jess.lucchetti@gmail.com. FIR - Zironda, Andrea IR - Zironda A IRAD- ASST GRANDE OSPEDALE METROPOLITANO NIGUARDA, Italy. Electronic address: And.zironda@gmail.com. FIR - De Stefano, Federico IR - De Stefano F IRAD- Division of Pancreatic Surgery, Pancreas Translational & Clinical Research Center, IRCCS San Raffaele Scientific Institute, Milan, Italy. Electronic address: destefano.federico@hsr.it. FIR - De Rose, Agostino Maria IR - De Rose AM IRAD- Hepatobiliary Surgery Unit, Fondazione Policlinico Universitario A.Gemelli IRCCS, Italy. Electronic address: agostinomaria.derose@policlinicogemelli.it. FIR - Esposito, Alessandro IR - Esposito A IRAD- Department of General and Pancreatic Surgery - Pancreas Unit, University Verona Hospital Trust, Verona, Italy. Electronic address: alessandro.esposito@aovr.veneto.it. FIR - Albanesi, Francesca IR - Albanesi F IRAD- Universita degli Studi di Mlano, Fondazione IRCCS Istituto Nazionale dei Tumori - Milano, MI, Italy. Electronic address: francesca.alba.96@gmail.com. FIR - La Vaccara, Vincenzo IR - La Vaccara V IRAD- UOC Chirurgia Generale Fondazione Policlinico Universitario Campus Bio-Medico, Italy. Electronic address: v.lavaccara@me.com. FIR - Zerbi, Alessandro IR - Zerbi A IRAD- Humanitas University, Italy. Electronic address: alessandro.zerbi@humanitas.it. FIR - Di Meo, Giovanna IR - Di Meo G IRAD- University of Bari, Italy. Electronic address: dimeojenny@gmail.com. FIR - Mereu, Antonfranco Luca Alessandro IR - Mereu ALA IRAD- Ospedale Michele e Pietro Ferrero, Italy. Electronic address: estreet75@libero.it. FIR - Iacomino, Alessandro IR - Iacomino A IRAD- Chirurgia 2 - Ca'Foncello Treviso, Italy. Electronic address: Iacominoalessandro@gmail.com. FIR - Cammarata, Roberto IR - Cammarata R IRAD- Fondazione Policlinico Universitario Campus Bio-Medico, Italy. Electronic address: roberto.cammarata@policlinicocampus.it. FIR - Corallino, Diletta IR - Corallino D IRAD- Department of General Surgery and Surgical Specialties, Sapienza University of Rome, Viale del Policlinico 155, Rome, 00161, Italy. Electronic address: dilettadile89@gmail.com. FIR - Del Prete, Luca IR - Del Prete L IRAD- IRCCS Fondazione Ca Granda Ospedale Maggiore Policlinico di Milano, Italy. Electronic address: luca.delpre90@gmail.com. FIR - Patrone, Renato IR - Patrone R IRAD- HPB department IRCCS Pascale of Naples, 80100, Italy. Electronic address: dott.patrone@gmail.com. FIR - Bianco, Giuseppe IR - Bianco G IRAD- Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Italy. Electronic address: giuseppe.bianco@policlinicogemelli.com. FIR - Capurso, Gabriele IR - Capurso G IRAD- San Raffaele Research Hospital, Italy. Electronic address: capurso.gabriele@hsr.it. FIR - Scopelliti, Michele IR - Scopelliti M IRAD- Ulss2 Treviso - Chirurgia 2, Italy. Electronic address: michele.scopelliti@aulss2.veneto.it. FIR - Yoshino, Jun IR - Yoshino J IRAD- Musashino Red Cross Hospital, Japan. Electronic address: junmsrg@tmd.ac.jp. FIR - Takemura, Nobuyuki IR - Takemura N IRAD- Saitama Medical Center, Saitama Medical University, Japan. Electronic address: ntake@saitama-med.ac.jp. FIR - Suzuki, Yasuyuki IR - Suzuki Y IRAD- Hyogo Prefectural Awaji Medical Center, Japan. Electronic address: szk@awajimc.jp. FIR - Minagawa, Masahiro IR - Minagawa M IRAD- Nagaoka Red Cross Hospital, Japan. Electronic address: mmina@nagaoka.jrc.or.jp. FIR - Satoi, Sohei IR - Satoi S IRAD- Dept of Pancreatobiliary Surgery, Kansai Medical University, Japan. Electronic address: satoi@hirakata.kmu.ac.jp. FIR - Hirano, Satoshi IR - Hirano S IRAD- Hokkaido University, Japan. Electronic address: satto@med.hokudai.ac.jp. FIR - Hirose, Yuki IR - Hirose Y IRAD- Niigata University Graduate School of Medical and Dental Sciences, Japan. Electronic address: y-hirose@med.niigata-u.ac.jp. FIR - Ninomiya, Riki IR - Ninomiya R IRAD- Saitama Medical Center, Saitama Medical University, Japan. Electronic address: rickynino9222@gmail.com. FIR - Anazawa, Takayuki IR - Anazawa T IRAD- Kyoto University, Japan. Electronic address: anazawa@kuhp.kyoto-u.ac.jp. FIR - Abu Hilal, Mohammad IR - Abu Hilal M IRAD- Department of Surgery, School of Medicine, The University of Jordan, Amman, Jordan. Electronic address: abuhilal9@gmail.com. FIR - Ilkul, Johnbosco H IR - Ilkul JH IRAD- Kenyatta National Hospital, Kenya. Electronic address: ILKULHAMAD@GMAIL.COM. FIR - Pukitis, Aldis IR - Pukitis A IRAD- University of Latvia and Pauls Stradins Clinical University Hospital in Riga, Latvia. Electronic address: pukitis@latnet.lv. FIR - Kvietkauskas, Mindaugas IR - Kvietkauskas M IRAD- Vilnius University Hospital Santaros Klinikos, Lithuania. Electronic address: mindaugas.kvietkauskas@santa.lt. FIR - Ignatavicius, Povilas IR - Ignatavicius P IRAD- Lithuanian University of Health Sciences, Lithuania. Electronic address: povilas.ignatavicius@lsmu.lt. FIR - Gulla, Aiste IR - Gulla A IRAD- Clinic of Surgery, Nephrology, Gastroenterology, Institute of Clinical Medicine, Faculty of medicine, Vilnius University, Lithuania. Electronic address: aistegulla@gmail.com. FIR - Fassari, Alessia IR - Fassari A IRAD- Centre Hospitalier de Luxembourg, Luxembourg. Electronic address: alessia.fassari@gmail.com. FIR - de Blasi, Vito IR - de Blasi V IRAD- Centre Hospitalier de Luxembourg, Luxembourg. Electronic address: dr.vdeblasi@gmail.com. FIR - Maia, Jose Costa IR - Maia JC IRAD- CHCSJ Macau SAR, Macau. Electronic address: costamaia57@gmail.com. FIR - Sellappan, Harivinthan IR - Sellappan H IRAD- Queen Elizabeth Hospital, Sabah, Malaysia. Electronic address: hari.vs@gmail.com. FIR - Yusoff, Ahmad Ramzi IR - Yusoff AR IRAD- Universiti Teknologi MARA, Malaysia. Electronic address: draramzy76@gmail.com. FIR - Koh, Peng Soon IR - Koh PS IRAD- Universiti Malaya, Malaysia. Electronic address: kohps2000@yahoo.com. FIR - Lee, Yeong Sing IR - Lee YS IRAD- Universiti Malaya Medical Centre, Malaysia. Electronic address: leeys@ummc.edu.my. FIR - Chapa, Oscar IR - Chapa O IRAD- Hospital General de Mexico en la Ciudad de Mexico, Mexico. Electronic address: clinicadepancreas@gmail.com. FIR - Miranda-Devora, Gerardo IR - Miranda-Devora G IRAD- ABC Medical Center, Mexico City, Mexico. Electronic address: drgmirandad@gmail.com. FIR - Ahmed, Bensaad IR - Ahmed B IRAD- Mohammed VI International University Hospital, Mohammed VI University of Sciences and Health, Casablanca, Morocco; Research Unit, Mohammed VI Center for Research and Innovation, Rabat, Morocco; Mohammed VI Faculty of Medicine, Mohammed VI University of Sciences and Health, Casablanca, Morocco. Electronic address: Dr.bensaad.ahmed@gmail.com. FIR - Pradhan, Sumita IR - Pradhan S IRAD- Tribhuvan University Teaching Hospital, Nepal. Electronic address: Sumiepradhan@gmail.com. FIR - Maharjan, Dhiresh Kumar IR - Maharjan DK IRAD- Kathmandu Medical College and Nepal Cancer Hospital, Nepal. Electronic address: maharjandhiresh@gmail.com. FIR - Lamichhane, Deep IR - Lamichhane D IRAD- Bhaktapur cancer hospital, Nepal. Electronic address: deeplamichhane@gmail.com. FIR - Chu, Michael Jen Jie IR - Chu MJJ IRAD- Laparoscopy Auckland, New Zealand. Electronic address: admin@drmichaelchu.co.nz. FIR - Soreide, Kjetil IR - Soreide K IRAD- Stavanger University Hospital, Norway. Electronic address: ksoreide@mac.com. FIR - Labori, Knut Jorgen IR - Labori KJ IRAD- Oslo University Hospital, Norway. Electronic address: k.j.labori@medisin.uio.no. FIR - Yan, Han IR - Yan H IRAD- The Affiliated BenQ Medical Center of Nanjing Medical University, China. Electronic address: carrick8862@163.com. FIR - El Achtar, Olivia IR - El Achtar O IRAD- Instituto Oncologico Nacional de Panama, Panama. Electronic address: draelachtar@gmail.com. FIR - Marcos, Juan Carlos IR - Marcos JC IRAD- Universidad Peruana de Ciencias Aplicadas, Blackwell University, Peru. Electronic address: jcmarcose@gmail.com. FIR - Nawacki, Lukasz IR - Nawacki L IRAD- The Jan Kochanowski University in Kielce, Poland. Electronic address: lukasz.nawacki@ujk.edu.pl. FIR - Zorniak, Michal IR - Zorniak M IRAD- The Maria Sklodowska-Curie National Research Institute of Oncology, Poland. Electronic address: michal.zorniak@gliwice.nio.gov.pl. FIR - Correia de Sa, Tiago IR - Correia de Sa T IRAD- Hepatobiliopancreatic Surgery and Transplantation Department, ULS Santo Antonio, Portugal. Electronic address: tiago.rc.sa@gmail.com. FIR - Castro Borges, Filipe IR - Castro Borges F IRAD- Botton-Champalimaud Pancreatic Cancer Centre. University if Lisbon, Portugal. Electronic address: filipe.borges@fundacaochampalimaud.pt. FIR - Del Rio Martin, Juan IR - Del Rio Martin J IRAD- Doctors Hospital Dorado/Orlando Health, Puerto Rico. Electronic address: juanvicente.delriomartin@gmail.com. FIR - Kim, Song Cheol IR - Kim SC IRAD- Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, Brain Korea 21 Project, Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea. Electronic address: drksc@amc.seoul.kr. FIR - Lee, Okjoo IR - Lee O IRAD- Soonchunhyang University Hospital, South Korea. Electronic address: altanis7@gmail.com. FIR - Marsico, Valentina IR - Marsico V IRAD- Asl roma 2, Italy. Electronic address: valentina.marsico@aslroma2.it. FIR - Octav, Ginghina IR - Octav G IRAD- Bucharest Institute of Oncology, Romania. Electronic address: octavginghina@gmail.com. FIR - Silvana Adelina, Gheorghe IR - Silvana Adelina G IRAD- Institute of Oncology Prof. Dr. Al. Trestioreanu Bucharest, Romania. Electronic address: adelina.silvana.gheorghe@gmail.com. FIR - Mardare, Mara IR - Mardare M IRAD- Bucharest Institute of Oncology, Romania. Electronic address: mara_mardare@yahoo.com. FIR - Dumitrascu, Traian IR - Dumitrascu T IRAD- Fundeni Clinical Institute, Carol Davila Univesity of Medicine and Pharmacy Bucharest Romania, Romania. Electronic address: traian.dumitrascu@umfcd.ro. FIR - Egorov, Viacheslav IR - Egorov V IRAD- Ilyinskaya Hospital, Russia. Electronic address: egorov12333@gmail.com. FIR - Egorov, Vasiliy IR - Egorov V IRAD- Kazan State Medical University, Russia. Electronic address: drvasiliy21@gmail.com. FIR - Zamora-Valdes, Daniel IR - Zamora-Valdes D IRAD- Hepatobiliary Sciences and Liver Transplantation Department, KASCH, KAMC, Riyadh, MNGHA, KSA, Saudi Arabia. Electronic address: dzamora@outlook.com. FIR - Karamarkovic, Aleksandar IR - Karamarkovic A IRAD- Faculty of Medicine Unicersity of Belgrade, Surgical Clinic UCC Zvezdara Belgrade, Serbia. Electronic address: alekara@sbb.ra. FIR - Bezmarevic, Mihailo IR - Bezmarevic M IRAD- Department of Hepatobiliary and Pancreatic Surgery, Clinic for General Surgery, University of Defense, Military Medical Academy, Belgrade, Serbia. Electronic address: bezmarevicm@gmail.com. FIR - Shelat, Vishal IR - Shelat V IRAD- Tan Tock Seng Hospital, Singapore. Electronic address: vgshelat@gmail.com. FIR - Khan, Zafar Ahmed IR - Khan ZA IRAD- Wits Donald Gordon Medical Centre, South Africa. Electronic address: zafcee@gmail.com. FIR - Jonas, Eduard IR - Jonas E IRAD- University of Cape Town, South Africa. Electronic address: eduard.jonas@uct.ac.za. FIR - Sardiwalla, Imraan IR - Sardiwalla I IRAD- Dr George Mukhari Academic Hospital, South Africa. Electronic address: imraansardiwalla@gmail.com. FIR - Noel, Colin IR - Noel C IRAD- University of the Free State. Universitas Academic Hospital, South Africa. Electronic address: drcbnoel@gmail.com. FIR - Kumar, Neha IR - Kumar N IRAD- Sefako Makgatho Health Sciences University, South Africa. Electronic address: kumarneha3004@gmail.com. FIR - Alsammani, Mohammedsu IR - Alsammani M IRAD- WITS UNIVERSITY, South Africa. Electronic address: alsammani21@gmail.com. FIR - Coetzee, Werner IR - Coetzee W FIR - Gonzalez Abos, Carolina IR - Gonzalez Abos C IRAD- Hospital Clinic de Barcelona, Spain. Electronic address: carol.gonzaleza@gmail.com. FIR - Serrablo, Alejandro IR - Serrablo A IRAD- Miguel Servet University Hospital, Spain. Electronic address: aserrablo@gmail.com. FIR - Serradilla-Martin, Mario IR - Serradilla-Martin M IRAD- Department of Surgery, Hospital Universitario Virgen de las Nieves, Granada, Spain. Electronic address: mserradilla@ugr.es. FIR - Domingo Del Pozo, Carlos IR - Domingo Del Pozo C IRAD- Hospital Universitaria Dr. Peset, Spain. Electronic address: domingocarlos@hotmail.com. FIR - Ausania, Fabio IR - Ausania F IRAD- Hospital Clinic Barcelona, Spain. Electronic address: f.ausania@googlemail.com. FIR - Lopez-Lopez, Victor IR - Lopez-Lopez V IRAD- Clinic and University Virgen de la Arrixaca Hospital. IMIB-Pascual Parrilla, Spain. Electronic address: victorrelopez@gmail.com. FIR - Bolado, Federico IR - Bolado F IRAD- Servicio de Aparato Digestivo, Hospital Universitario de Navarra, Spain. Electronic address: fboladoc@cfnavarra.es. FIR - Luzjuez, Luz Divina Juez IR - Luzjuez LDJ IRAD- Hospital Universitario Ramon y Cajal. IRYCIS. Madrid, Spain. Electronic address: luz.juez@gmail.com. FIR - Ramia, Jose M IR - Ramia JM IRAD- Hospital General Universitario Dr. Balmis, Spain. Electronic address: jose_ramia@hotmail.com. FIR - Sanchez Cabus, Santiago IR - Sanchez Cabus S IRAD- Hospital de la Santa Creu i Sant Pau, Spain. Electronic address: ssanchezca@santpau.cat. FIR - Conde, Belen IR - Conde B IRAD- E-AHPBA, Switzerland. Electronic address: bciconde@gmail.com. FIR - Halimi, Asif IR - Halimi A IRAD- Umea university, Sweden. Electronic address: Asif.halimi@umu.se. FIR - Sparrelid, Ernesto IR - Sparrelid E IRAD- Division of Surgery and Oncology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden. Electronic address: ernesto.sparrelid@ki.se. FIR - Rangelova, Elena IR - Rangelova E IRAD- Sahlgrenska University Hospital, University of Gothenburg, Sweden. Electronic address: elena.rangelova@gu.se. FIR - Gkekas, Ioannis IR - Gkekas I IRAD- Umea University, Faculty of Medicine, Department of Diagnostics and Intervention, Sweden. Electronic address: gkekasioannis@windowslive.com. FIR - Bergthor, Bjornsson IR - Bergthor B IRAD- Linkoping University, Sweden. Electronic address: Bergthor.Bjornsson@liu.se. FIR - Andersson, Bodil IR - Andersson B IRAD- Lund University and Skane University Hospital, Lund, Sweden. Electronic address: bodil.andersson@med.lu.se. FIR - Bereza-Carlson, Paulina IR - Bereza-Carlson P IRAD- Lunds University, Sweden. Electronic address: Paulina.bereza-carlson@med.lu.se. FIR - Valente, Roberto IR - Valente R IRAD- Department of Diagnostics and Intervention, Surgery, Umea University, Umea, Sweden. Electronic address: roberto.valente@umu.se. FIR - Scandavini, Chiara Maria IR - Scandavini CM IRAD- Karolinska University Hospital, Sweden. Electronic address: chiara.scanda@hotmail.it. FIR - Halimi, Asif IR - Halimi A IRAD- Umea university, Sweden. Electronic address: asif_halimi@hotmail.com. FIR - Sotirova, Ira IR - Sotirova I IRAD- Umea university, Sweden. Electronic address: Ira.sotirova@gmail.com. FIR - Kuemmerli, Christoph IR - Kuemmerli C IRAD- Clarunis University Digestive Health Care Center, University Hospital Basel, Basel, Switzerland. Electronic address: christoph.kuemmerli@clarunis.ch. FIR - Adrian, Billeter IR - Adrian B IRAD- Clarunis University Digestive Healthcare Center Basel, Switzerland. Electronic address: adrian.billeter@clarunis.ch. FIR - De Dosso, Sara IR - De Dosso S IRAD- Oncology Institute of Southern Switzerland, Switzerland. Electronic address: sara.dedosso@eoc.ch. FIR - Eshmuminov, Dilmurodjon IR - Eshmuminov D IRAD- Department of Surgery, Univercity Hospital Zurich, Switzerland. Electronic address: dilmurodjon.eshmuminov@usz.ch. FIR - Duro, Francesca IR - Duro F IRAD- Ospedale Santa Maria Terni, Italy. Electronic address: durofrancesca3@gmail.com. FIR - Keeratibharat, Nattawut IR - Keeratibharat N IRAD- School of Surgery, Institute of Medicine, Suranaree University of Technology, Thailand. Electronic address: nattawut1986@gmail.com. FIR - Tovikkai, Chutwichai IR - Tovikkai C IRAD- Faculty of Medicine Siriraj Hospital, Thailand. Electronic address: chutwichai.tov@mahidol.ac.th. FIR - de Meijer, Vincent E IR - de Meijer VE IRAD- Department of Surgery, University of Groningen and University Medical Center Groningen, Groningen, the Netherlands. Electronic address: v.e.de.meijer@umcg.nl. FIR - Clarysse, Mathias IR - Clarysse M IRAD- Maastricht UMC+, the Netherlands. Electronic address: mathias.clarysse@mumc.nl. FIR - Besselink, Marc Mg IR - Besselink MM IRAD- Amsterdam UMC, location University of Amsterdam, Department of Surgery, Amsterdam, the Netherlands. Electronic address: m.g.besselink@amsterdamumc.nl. FIR - Bozkurt, Emre IR - Bozkurt E IRAD- Department of General Surgery, Koc University School of Medicine, Turkey. Electronic address: dr.emrebozkurt@gmail.com. FIR - Canturk, Alp Omer IR - Canturk AO IRAD- Sakarya University Training and Resarch Hospital, Turkey. Electronic address: dr.omercanturk@gmail.com. FIR - Uludag, Gokhan IR - Uludag G FIR - Coker, Ahmet IR - Coker A IRAD- Ege University and Medicana Int Izmir Hospital, Turkey. Electronic address: ahmet.coker@ege.edu.tr. FIR - Serin, Kursat IR - Serin K IRAD- Istanbul University HPB Surgery Department, Turkey. Electronic address: dr_krserin@hotmail.com. FIR - Okut, Gokalp IR - Okut G IRAD- Health Science University Izmir City Hospital, Turkey. Electronic address: gokalp.okut@gmail.com. FIR - Ugur, Mehmet Alperen IR - Ugur MA IRAD- Saglik Bilimleri Universitesi, Turkey. Electronic address: mehmet.alperen.ugur@gmail.com. FIR - Yilmaz, Inan IR - Yilmaz I IRAD- E-AHPBA, Switzerland. Electronic address: inan2701@gmail.com. FIR - Benk, Mehmet Sah IR - Benk MS IRAD- Mugla Training and Research Hospital, Turkey. Electronic address: dr.m.sah@gmail.com. FIR - Sonmez, Recep Ercin IR - Sonmez RE IRAD- Istanbul Medeniyet University Goztepe Prof Dr Suleyman Yalcin City Hospital, Turkey. Electronic address: sonmezercin@gmail.com. FIR - Ozcan, Onder IR - Ozcan O IRAD- MUGLA SITKI KOCMAN UNIVERSTY, Turkey. Electronic address: onderozan@mu.edu.tr. FIR - Karabulut, Kagan IR - Karabulut K IRAD- Ondokuz mayis university, Turkey. Electronic address: Kagankarabulut@hotmail.com. FIR - Acar, Serdar IR - Acar S IRAD- Suleyman Demirel University, Turkey. Electronic address: dr.serdaracar@gmail.com. FIR - Hawkins, Maria IR - Hawkins M IRAD- University College London, United Kingdom. Electronic address: m.hawkins@ucl.ac.uk. FIR - Pandanaboyana, Sanjay IR - Pandanaboyana S FIR - Sutcliffe, Robert IR - Sutcliffe R IRAD- University Hospital Birmingham NHS Trust, United Kingdom. Electronic address: robert.sutcliffe@uhb.nhs.uk. FIR - Thakkar, Rohan IR - Thakkar R IRAD- Freeman hospital, Necastle upon Tyne, United Kingdom. Electronic address: rohan.thakkar@nhs.net. FIR - Bandyopadhyay, Samik IR - Bandyopadhyay S IRAD- Manchester Royal Infirmary, United Kingdom. Electronic address: drsamikbanerjee@yahoo.com. FIR - Chatzizacharias, Nikolaos IR - Chatzizacharias N IRAD- Queen Elizabeth Hospital Birmingham, United Kingdom. Electronic address: chatzizacharias@gmail.com. FIR - Daniels, Sarah IR - Daniels S IRAD- Sheffield TEaching hospitals, United Kingdom. Electronic address: sarahdaniels1@nhs.net. FIR - Motelchuk, Serhii IR - Motelchuk S IRAD- Shalimov National Scientific Center for Surgery and Transplantology, Kyiv, Ukraine. Electronic address: dr.motelchuk@gmail.com. FIR - Sumarokova, Valeriia IR - Sumarokova V IRAD- Kyiv City Oncology Center, Ukraine. Electronic address: valeriia.sumarokova@gmail.com. FIR - McDonnell, Declan IR - McDonnell D IRAD- University Hospital Southampton, United Kingdom. Electronic address: declan@doctors.org.uk. FIR - Fusai, Giuseppe Kito IR - Fusai GK IRAD- HPB & Liver Transpkant Unit - Royal Free Hospital, United Kingdom. Electronic address: G.fusai@nhs.net. FIR - Mavroeidis, Vasileios K IR - Mavroeidis VK IRAD- NBT & UHBW, Bristol, United Kingdom. Electronic address: blackbasildr@yahoo.gr. FIR - Szatmary, Peter IR - Szatmary P IRAD- Liverpool University Hospitals NHS Foundation Trust, United Kingdom. Electronic address: szatmary@liverpool.ac.uk. FIR - Spiers, Harry IR - Spiers H IRAD- Department of Hepato-pancreato-biliary Surgery, Addenbrooke's Hospital, Cambridge, United Kingdom; Department of Surgery, University of Cambridge, Cambridge, United Kingdom. Electronic address: hs781@cam.ac.uk. FIR - Mowbray, Nick IR - Mowbray N IRAD- Morriston Hospital, Swansea, United Kingdom. Electronic address: nicholas.mowbray@wales.nhs.uk. FIR - Bynon, J Steve Jr IR - Bynon JS Jr IRAD- McGovern School of Medicine University of Texas Houston, United Kingdom. Electronic address: john.s.bynon@uth.tmc.edu. FIR - Stauffer, John IR - Stauffer J IRAD- Mayo Clinic Florida, United States. Electronic address: stauffer.john@mayo.edu. FIR - Kunzler, Filipe IR - Kunzler F IRAD- Miami Cancer Institute, United States. Electronic address: kunzler.filipe@gmail.com. FIR - Ocuin, Lee IR - Ocuin L IRAD- Lehigh Valley Health Network, United States. Electronic address: lee.ocuinmd@gmail.com. FIR - Bader, Amanda IR - Bader A IRAD- University of Pennsylvania, United States. Electronic address: amanda.bader@pennmedicine.upenn.edu. FIR - Moris, Dimitrios IR - Moris D IRAD- Medstar Georgetown Transplant Institute, United States. Electronic address: dimmoris@yahoo.com. FIR - Beal, Eliza IR - Beal E IRAD- Karmanos Cancer Institute/Wayne State University, United States. Electronic address: Beale@karmanos.org. FIR - St Julien, Jamii IR - St Julien J IRAD- HCA Florida Trinity Hospital, United States. Electronic address: jamii4@gmail.com. FIR - Forrester, Jared IR - Forrester J IRAD- Hoag Family Cancer Institute, United States. Electronic address: jforrester9@gmail.com. FIR - Melstrom, Laleh IR - Melstrom L IRAD- City of Hope National Medical Center, United States. Electronic address: lmelstrom@coh.org. FIR - Nassour, Ibrahim IR - Nassour I IRAD- University of Florida, United States. Electronic address: Nassour.ibrahim@gmail.com. FIR - Moaven, Omeed IR - Moaven O IRAD- Louisiana State University, New Orleans, United States. Electronic address: omoave@lsuhsc.edu. FIR - William, Helton IR - William H IRAD- Virginia Mason Medical Center, United States. Electronic address: scott.helton@commonspirit.org. FIR - Imagawa, David K IR - Imagawa DK IRAD- University of California, Irvine Medical Center, United States. Electronic address: dkimagaw@hs.uci.edu. FIR - Fong, Zhi Ven IR - Fong ZV IRAD- Mayo Clinic Arizona, United States. Electronic address: Fong.Zhi@mayo.edu. FIR - Schmocker, Ryan IR - Schmocker R IRAD- University of Tennessee Graduate School of Medicine, United States. Electronic address: rkschmocker@gmail.com. FIR - Bonds, Morgan IR - Bonds M IRAD- OU Health College of Medicine, United States. Electronic address: morgan-bonds@ouhsc.edu. FIR - Pineda, Jaime Pineda IR - Pineda JP FIR - McLoughlin, James IR - McLoughlin J FIR - Lowy, Andrew IR - Lowy A IRAD- UC San Diego, United States. Electronic address: alowy@ucsd.edu. FIR - Zeoli, Mariana IR - Zeoli M IRAD- Department of Vascular SurgeryMontevideo, Uruguay. Electronic address: marianazeoli@yahoo.com.ar. FIR - Ross, Sharona IR - Ross S IRAD- AdventHealth Tampa, United States. Electronic address: Mysharonaross@gmail.com. FIR - Etherington, Mark IR - Etherington M IRAD- University of Pennsylvania, United States. Electronic address: Marketh@pennmedicine.UPenn.edu. FIR - Ferguson, Ben IR - Ferguson B IRAD- University of Michigan, United States. Electronic address: benferg@med.umich.edu. FIR - Serrano, Oscar IR - Serrano O IRAD- Hartford Hospital/University of Connecticut, United States. Electronic address: oscar.serrano@hhchealth.org. FIR - Galka, Eva IR - Galka E IRAD- Strong Memorial Hospital General Surgery - Rochester, United States. Electronic address: Eva_Galka@urmc.rochester.edu. FIR - Ecker, Brett L IR - Ecker BL IRAD- Division of Surgical Oncology, Department of Surgery, Northwestern University Feinberg School of Medicine, Chicago, IL, United States. Electronic address: brettecker@gmail.com. FIR - Correa, Camilo IR - Correa C IRAD- Mount Sinai Health System, United States. Electronic address: Camilo.Correa@mountsinai.org. FIR - Javed, Ammar IR - Javed A IRAD- NYU Langone Health, United States. Electronic address: ammar.javed@nyulangone.org. FIR - Peng, June IR - Peng J IRAD- University of California San Francisco, United States. Electronic address: june.peng@ucsf.edu. FIR - Chirichella, Thomas IR - Chirichella T IRAD- Mercy Health St. Elizabeth Youngstown Hospital, United States. Electronic address: tchirichella@gmail.com. FIR - Davis, Catherine IR - Davis C IRAD- Baylor Univ Med Center, United States. Electronic address: Chambletondavis@gmail.com. FIR - Pawlik, Timothy IR - Pawlik T IRAD- The Ohio State Wexner Medical Center, United States. Electronic address: tim.pawlik@osumc.edu. FIR - Golas, Benjamin IR - Golas B IRAD- Hackensack Meridian Health System, United States. Electronic address: bengolas1@gmail.com. FIR - Sanabria, Juan IR - Sanabria J IRAD- Marshall and CaaSA, United States. Electronic address: juan.sanabria@case.edu. FIR - Kleinman, Michael IR - Kleinman M IRAD- Memorial Hermann Southwest Hospital, United States. Electronic address: M.kleinman@comcast.net. FIR - Desai, Darius IR - Desai D IRAD- St. Luke's University Health Network, United States. Electronic address: dcdesai01@gmail.com. FIR - Sastry, Amit IR - Sastry A IRAD- Summa Health Akron City Hospital, United States. Electronic address: amit.sastry@gmail.com. FIR - Schrope, Beth IR - Schrope B IRAD- Columbia University, United States. Electronic address: bs170@cumc.columbia.edu. FIR - Stackhouse, Katie IR - Stackhouse K IRAD- Cleveland Clinic Akron General, United States. Electronic address: kstackhouse2@gmail.com. FIR - Kennedy, Timothy IR - Kennedy T IRAD- Georgetown University MedStar Health, United States. Electronic address: tkennedyhoya@yahoo.com. FIR - Vollmer, Charles IR - Vollmer C IRAD- University of Pennsylvania, United States. Electronic address: Charles.vollmer@uphs.UPenn.edu. FIR - Pasko, Jennifer IR - Pasko J IRAD- Providence Sacred Heart, United States. Electronic address: pasko.jen@gmail.com. FIR - White, Michael IR - White M IRAD- Bon Secours Surgical Specialists at St. Mary's Hospital, Richmond, VA, United States. Electronic address: Michael_White3@bshsi.org. FIR - Nguyen, Thanh Sang IR - Nguyen TS IRAD- TRUNG VUONG hospital, Vietnam. Electronic address: Drthanhsangnguyenbvtv@gmail.com. FIR - Chihaka, Onesai IR - Chihaka O IRAD- Gastroenterology clinic Harare, Zimbabwe. Electronic address: obchihaka@gmail.com. FIR - Chooklin, Serge IR - Chooklin S IRAD- Lviv Regional Clinical Hospital, Ukraine. Electronic address: chooklin_serge@hotmail.com. FIR - Christophides, Thalis IR - Christophides T EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:12 PHST- 2026/07/10 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:12 [entrez] AID - S0960-7404(26)00231-8 [pii] AID - 10.1016/j.suronc.2026.102580 [doi] PST - aheadofprint SO - Surg Oncol. 2026 Oct 26;69:102580. doi: 10.1016/j.suronc.2026.102580. PMID- 42950762 OWN - NLM STAT- Publisher LR - 20261028 IS - 1618-1328 (Electronic) IS - 0176-1617 (Linking) VI - 327 DP - 2026 Oct 19 TI - Analytical techniques and method-dependent biases in micro- and nanoplastics research in plant systems. PG - 154900 LID - S0176-1617(26)00213-0 [pii] LID - 10.1016/j.jplph.2026.154900 [doi] AB - Microplastics and nanoplastics are increasingly recognized as emerging contaminants in agroecosystems, yet their interactions with plant systems remain insufficiently understood. Recent evidence indicates that plants can take up and accumulate microplastics and nanoplastics through roots and foliar exposure, leading to their accumulation in different tissues and raising concerns about potential impacts on plant physiology and food safety. However, elucidating these processes is strongly constrained by methodological limitations arising from complex plant matrices, heterogeneous particle distribution, and the detection of small-sized plastics. This review focuses on current and emerging techniques for the detection of micro- and nanoplastics in plant systems, including vibrational spectroscopy, mass spectrometry, and imaging techniques. These key methods are assessed in the context of plant-specific applications, with emphasis on their capabilities for quantification, size resolution, and spatial localization, as well as their limitations arising from plant matrix interference. By highlighting key methodological challenges and future needs, this review aims to provide practical guidance for method selection and promote more reliable, comparable, and standardized research on microplastics and nanoplastics in plants. CI - Copyright (c) 2026 Elsevier GmbH. All rights reserved. FAU - Li, Mingyang AU - Li M AD - State Key Laboratory of Efficient Production of Forest Resource, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China. FAU - Liu, Yanwei AU - Liu Y AD - Laboratory of Environmental Nanotechnology and Health Effect, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China. FAU - Zhang, Guifang AU - Zhang G AD - State Key Laboratory of Efficient Production of Forest Resource, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China. FAU - Qin, Yu AU - Qin Y AD - State Key Laboratory of Efficient Production of Forest Resource, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China. FAU - Zhang, Shili AU - Zhang S AD - State Key Laboratory of Efficient Production of Forest Resource, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China. FAU - Wu, Hongyang AU - Wu H AD - State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100193, China. FAU - Lin, Jinxing AU - Lin J AD - State Key Laboratory of Efficient Production of Forest Resource, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China; Yazhouwan National Laboratory, Sanya, 570024, China. FAU - Li, Xiaojuan AU - Li X AD - State Key Laboratory of Efficient Production of Forest Resource, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China. Electronic address: lixj@bjfu.edu.cn. LA - eng PT - Journal Article DEP - 20261019 PL - Germany TA - J Plant Physiol JT - Journal of plant physiology JID - 9882059 SB - IM OTO - NOTNLM OT - Analytical methods OT - Method-dependent biases OT - Microplastics OT - Nanoplastics OT - Plant uptake and translocation COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:12 PHST- 2026/08/04 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:12 [entrez] AID - S0176-1617(26)00213-0 [pii] AID - 10.1016/j.jplph.2026.154900 [doi] PST - aheadofprint SO - J Plant Physiol. 2026 Oct 19;327:154900. doi: 10.1016/j.jplph.2026.154900. PMID- 42950757 OWN - NLM STAT- Publisher LR - 20261028 IS - 1532-3072 (Electronic) IS - 0040-8166 (Linking) VI - 104 IP - Pt 3 DP - 2026 Oct 22 TI - Selective expression and neurochemical characterization of Huntingtin-associated protein 1-expressing neurons in the adult mouse habenula. PG - 104078 LID - S0040-8166(26)00774-3 [pii] LID - 10.1016/j.tice.2026.104078 [doi] AB - Huntingtin-associated protein 1 (HAP1) is a neuroprotective interactor with pathogenic proteins implicated in several neurodegenerative diseases. The habenula is a small epithalamic structure that has emerged as a critical regulator of aversion, reward, stress responses, anxiety, and mood. Despite its pivotal roles in intracellular trafficking and neuronal function, the distribution and neurochemical characterization of HAP1 within the habenula have not been systematically investigated. Therefore, the present study used Western blotting and immunohistochemistry to characterize HAP1 immunoreactivity in the adult mouse habenula. Our current study revealed that HAP1-immunoreactive (ir) neurons were selectively localized to the lateral habenula (LHb), whereas no detectable HAP1 immunoreactivity was observed in the medial habenula. Within the LHb, HAP1-ir neurons were concentrated in the LHbMC, LHbMPc, and LHbMS subdivisions. Double-label immunofluorescence revealed that 9% of HAP1-positive neurons expressed choline acetyltransferase and 22% expressed neuronal nitric oxide synthase. Moderate proportions expressed calbindin (52%) and calretinin (59%), whereas the highest proportions expressed calcitonin gene-related peptide (84%) and cholecystokinin (74%). No detectable co-localization was observed between HAP1 and parvalbumin. Substance P immunoreactivity was observed in fibers rather than neuronal somata within the analyzed LHb region, precluding quantitative assessment of neuronal co-expression. These findings demonstrate that HAP1-ir neurons exhibit distinct neurochemical profiles, particularly involving peptidergic and calcium-binding protein-expressing neuronal populations. These selective co-expression profiles highlight the neurochemical heterogeneity of HAP1-ir neurons within the LHb and provide an anatomical framework for future investigations of HAP1 function in habenular circuitry. CI - Copyright (c) 2026 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Meher, Mirza Mienur AU - Meher MM AD - Department of Basic Laboratory Sciences, Faculty of Medicine and Health Sciences, Yamaguchi University Graduate School of Medicine, 1‑1‑1 Minami‑Kogushi, Ube, 755‑8505, Japan; Division of Neuroanatomy, Department of Neuroscience, Yamaguchi University Graduate School of Medicine, 1‑1‑1 Minami‑Kogushi, Ube, 755‑8505, Japan; Department of Microbiology and Public Health, Faculty of Veterinary Medicine and Animal Science, Gazipur Agricultural University, Gazipur, 1706, Bangladesh. FAU - Islam, Md Nabiul AU - Islam MN AD - Division of Neuroanatomy, Department of Neuroscience, Yamaguchi University Graduate School of Medicine, 1‑1‑1 Minami‑Kogushi, Ube, 755‑8505, Japan. Electronic address: nabiul@yamaguchi-u.ac.jp. FAU - Afrin, Marya AU - Afrin M AD - Division of Neuroanatomy, Department of Neuroscience, Yamaguchi University Graduate School of Medicine, 1‑1‑1 Minami‑Kogushi, Ube, 755‑8505, Japan; Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, 2202, Bangladesh. FAU - Yanai, Akie AU - Yanai A AD - Department of Basic Laboratory Sciences, Faculty of Medicine and Health Sciences, Yamaguchi University Graduate School of Medicine, 1‑1‑1 Minami‑Kogushi, Ube, 755‑8505, Japan; Division of Neuroanatomy, Department of Neuroscience, Yamaguchi University Graduate School of Medicine, 1‑1‑1 Minami‑Kogushi, Ube, 755‑8505, Japan. Electronic address: akiey@yamaguchi-u.ac.jp. FAU - Shinoda, Koh AU - Shinoda K AD - Division of Neuroanatomy, Department of Neuroscience, Yamaguchi University Graduate School of Medicine, 1‑1‑1 Minami‑Kogushi, Ube, 755‑8505, Japan; School of Human Care Studies, Nagoya University of Arts and Sciences, 57 Takenoyama, Iwasaki-cho, Nisshin-shi, Aichi, 470-0196, Japan. Electronic address: shinoda@nuas.ac.jp. LA - eng PT - Journal Article DEP - 20261022 PL - Scotland TA - Tissue Cell JT - Tissue & cell JID - 0214745 SB - IM OTO - NOTNLM OT - Calcium-binding proteins OT - HAP1 OT - Habenula OT - Neuropeptides OT - Neuropsychiatric disorders OT - Stress circuitry COIS- Declaration of Competing Interest The authors declare that they have no conflicts of interest that could have influenced this work. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:12 PHST- 2026/01/07 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:12 [entrez] AID - S0040-8166(26)00774-3 [pii] AID - 10.1016/j.tice.2026.104078 [doi] PST - aheadofprint SO - Tissue Cell. 2026 Oct 22;104(Pt 3):104078. doi: 10.1016/j.tice.2026.104078. PMID- 42950754 OWN - NLM STAT- Publisher LR - 20261028 IS - 2468-2942 (Electronic) IS - 2468-2942 (Linking) VI - 49 DP - 2026 Oct 26 TI - Patterns of high-risk histopathology features of retinoblastoma in enucleated and exenterated eyes at Tikur Anbessa Specialized Hospital: a retrospective study. PG - 101506 LID - S2468-2942(26)00415-6 [pii] LID - 10.1016/j.ctarc.2026.101506 [doi] AB - INTRODUCTION: Retinoblastoma (RB) is a common eye tumor in children, particularly in developing countries, where it is often diagnosed at advanced stages. Early detection is crucial for successful treatment, as untreated RB can be fatal. A study conducted at Tikur Anbessa Specialized Hospital (TASH) aimed to assess the rate of high-risk histopathological features (HRF) in RB patients. METHODS: Institution based retrospective review was conducted. Data was collected from patients' pathology reports. The data was entered and analyzed using SPSS version 26 data analysis software. RESULT: The study involved 110 children, with a mean age of 36.33 months (SD = 20.6), a minimum age of 4 months, and a maximum age of 120 months. Most cases (98, 89.1%) were diagnosed before the age of 4 years. Gross findings were evaluated using 1 to 3 blocks in 33 (50.8%) of the cases, and the most common histopathological feature observed was necrosis, present in 91 (82.7%) of the cases. High-risk features (HRF) were observed in 62 (56.4%) of the patients, with the most frequent HRF being postlaminar optic nerve invasion (28, 25.5%), followed by extrascleral invasion (27, 24.5%). The age of the patient was significantly correlated with HRF, with a positive correlation coefficient of 0.187 a p-value of 0.025. CONCLUSION: A significant number of patients having RB assessed at TASH showed a high frequency of HRF. This emphasizes the importance of early detection and comprehensive histopathology reporting to improve patient outcomes. CI - Copyright (c) 2026 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Wubalem, Seblewengel Maru AU - Wubalem SM AD - Department of Pathology, Tikur Anbessa Specialized Hospital, Addis Ababa University, Addis Ababa, Ethiopia. Electronic address: maruseblewengel@gmail.com. FAU - Belay, Winini AU - Belay W AD - Department of Reproductive Health and Health Service Management, School of Public Health, Addis Ababa University, Addis Ababa, Ethiopia. Electronic address: wininiquidnac@gmail.com. FAU - Yirgalem, Kaleab AU - Yirgalem K AD - Department of Pathology, Hawassa University Comprehensive Specialized Hospital, Hawassa, Sidama, Ethiopia. Electronic address: kaleabyirge@gmail.com. FAU - Mengesha, Tariku AU - Mengesha T AD - Armauer Hansen research institute, Addis Ababa, Ethiopia. Electronic address: newtariku@gmail.com. FAU - Ergete, Wondwossen AU - Ergete W AD - Department of Pathology, Tikur Anbessa Specialized Hospital, Addis Ababa University, Addis Ababa, Ethiopia. Electronic address: wondwossen_ergete@yahoo.com. LA - eng PT - Journal Article DEP - 20261026 PL - England TA - Cancer Treat Res Commun JT - Cancer treatment and research communications JID - 101694651 SB - IM OTO - NOTNLM OT - Children OT - Eye tumor OT - High risk histopathology feature OT - Retinoblastoma COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:12 PHST- 2025/11/11 00:00 [received] PHST- 2026/10/25 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:12 [entrez] AID - S2468-2942(26)00415-6 [pii] AID - 10.1016/j.ctarc.2026.101506 [doi] PST - aheadofprint SO - Cancer Treat Res Commun. 2026 Oct 26;49:101506. doi: 10.1016/j.ctarc.2026.101506. PMID- 42950752 OWN - NLM STAT- Publisher LR - 20261028 IS - 2468-2942 (Electronic) IS - 2468-2942 (Linking) VI - 49 DP - 2026 Oct 26 TI - Probiotic modulation of colorectal cancer stemness and metastasis: mechanisms and therapeutic implications. PG - 101504 LID - S2468-2942(26)00413-2 [pii] LID - 10.1016/j.ctarc.2026.101504 [doi] AB - Colorectal cancer (CRC) remains a major cause of cancer-related mortality, with metastasis, therapeutic resistance, and the persistence and plasticity of cancer stem cells (CSCs) contributing to treatment failure. This review evaluates evidence linking defined probiotic strains, multispecies formulations, and other microbiota-directed interventions to CRC stemness and metastatic biology. Because effects vary according to strain identity, viability, dose, formulation, host context, and experimental model, findings from one microorganism or product cannot be generalized across probiotics as a class. Most available studies do not demonstrate direct targeting of colorectal CSCs. Instead, reported effects are predominantly indirect and involve changes in microbiota composition, microbial metabolites, inflammatory and immune signaling, epithelial barrier integrity, and the tumor microenvironment. A smaller group of mechanistic studies identifies strain- and context-specific effects on stemness-related pathways. Animal studies also report effects on intestinal barrier integrity, tumor growth, angiogenesis, immune-cell composition, treatment-associated toxicity, and experimental treatment sensitivity, but these findings remain preclinical. Human evidence is limited and mainly concerns gastrointestinal symptoms, microbiota changes, and treatment tolerance. None of the human studies identified in this review demonstrated that probiotic supplementation improves objective tumor response, progression-free survival, overall survival, metastasis-free survival, or checkpoint-immunotherapy efficacy in CRC. Accordingly, the current literature supports biological plausibility for selected, well-characterized microbial interventions rather than a class-wide anticancer effect of probiotics. Direct CSC eradication, prevention of metastatic recurrence, and reproducible enhancement of chemotherapy/immunotherapy efficacy remain clinically unproven. Future studies require strain-resolved reporting, standardized dosing and formulation, mechanistic validation, and adequately powered clinical trials with oncologic endpoints. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved. FAU - Oriquat, Ghaleb AU - Oriquat G AD - Faculty of Allied Medical Sciences, Hourani Center for Applied Scientific Research, Al-Ahliyya Amman University, Amman, Jordan. Electronic address: goreqat@ammanu.edu.jo. FAU - Rizaev, Jasur AU - Rizaev J AD - Department of Public Health and Healthcare management, Rector, Samarkand State Medical University, Samarkand, Uzbekistan. Electronic address: rizaevjasur@yahoo.com. FAU - Kadhim, Amir Abdul AU - Kadhim AA AD - Department of Medical Laboratory Technologies, College of Medical Technology, The Islamic University, Najaf, Iraq. Electronic address: Amir.Abdul.Kadhim@iunajaf.edu.iq. FAU - Jamuna, K V AU - Jamuna KV AD - Department of Forensic Science, School of Sciences, JAIN (Deemed to be University), Bangalore, Karnataka, India. Electronic address: kv.jamuna@jainuniversity.ac.in. FAU - Maharana, Laxmidhar AU - Maharana L AD - Department of Pharmaceutical Sciences, Siksha 'O' Anusandhan (Deemed to be University), Bhubaneswar, Odisha, 751030, India. Electronic address: laxmidharmaharana@soa.ac.in. FAU - Bainsal, Neeraj AU - Bainsal N AD - University Institute of Pharma Sciences, Chandigarh University, Mohali, Punjab, India. Electronic address: neeraj012025@outlook.com. FAU - Singhal, Divya AU - Singhal D AD - Centre for Research Impact and Outcome, Chitkara University, Rajpura, Punjab, India; Sharda School of Bio-Science & Technology, Sharda University, Greater Noida, India. Electronic address: divyasinghal2026@outlook.com. FAU - Polatova, Djamila AU - Polatova D AD - Scientific-Practical Medical Center for Pediatric Oncology, Hematology and Immunology, Tashkent, Uzbekistan. Electronic address: djamilapolatova@zohomail.com. LA - eng PT - Journal Article PT - Review DEP - 20261026 PL - England TA - Cancer Treat Res Commun JT - Cancer treatment and research communications JID - 101694651 SB - IM OTO - NOTNLM OT - Cancer stem cells OT - Colorectal cancer OT - Metastasis OT - Probiotics OT - Tumor microenvironment COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:12 PHST- 2026/07/12 00:00 [received] PHST- 2026/08/18 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:12 [entrez] AID - S2468-2942(26)00413-2 [pii] AID - 10.1016/j.ctarc.2026.101504 [doi] PST - aheadofprint SO - Cancer Treat Res Commun. 2026 Oct 26;49:101504. doi: 10.1016/j.ctarc.2026.101504. PMID- 42950751 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-2550 (Electronic) IS - 0304-4017 (Linking) VI - 349 DP - 2026 Oct 26 TI - Identification of a promising benzothiadiazole-derived compound with larvicidal activity against Toxocara canis: in vitro, in silico and in vivo evaluation. PG - 110966 LID - S0304-4017(26)00285-2 [pii] LID - 10.1016/j.vetpar.2026.110966 [doi] AB - Toxocariasis is a globally distributed zoonotic disease caused primarily by infection with Toxocara canis, whose larvae migrate through host tissues. Therapeutic options for tissue toxocariasis remain limited, highlighting the need for the development of new antiparasitic compounds. Benzothiadiazole derivatives have attracted increasing interest because of their biological applications and their potential for the identification of biologically active compounds. In this study, fourteen synthetic derivatives from the bis-arylsulfenyl-benzo-2,1,3-thiadiazole series were evaluated through in vitro, in silico, and in vivo approaches. Initial screening identified four compounds with complete larvicidal activity at a concentration of 1 mg/mL. Determination of the minimum larvicidal concentration and cytotoxicity assays using J774A.1 murine macrophages enabled the selection of compounds C3 and C9 for further investigation. Both compounds exhibited larvicidal activity and low cytotoxicity at the concentrations tested. Confirmation assays demonstrated the absence of larval recovery in animals inoculated with larvae previously exposed to either compound, indicating loss of infectivity. In silico analyses revealed physicochemical properties compatible with good oral bioavailability. In a murine model of toxocariasis, treatment with compound C9 significantly reduced parasite burden compared with the untreated control group and exhibited efficacy comparable to that of albendazole. In contrast, compound C3 did not demonstrate significant in vivo activity. These findings identify C9 as a promising candidate for the development of new therapeutic alternatives against toxocariasis and support further studies to investigate its pharmacological properties and therapeutic potential. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Faria, Andrezza Medeiros AU - Faria AM AD - Laboratory of Parasitology, School of Medicine, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil. Electronic address: andrezzamedeiros@hotmail.com. FAU - Netto de Oliveira da Cunha, Carolina AU - Netto de Oliveira da Cunha C AD - Laboratory of Parasitology, School of Medicine, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil. FAU - Panassolo, Victoria Pires AU - Panassolo VP AD - Laboratory of Parasitology, School of Medicine, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil. FAU - Martins, Lourdes Helena Rodrigues AU - Martins LHR AD - Laboratory of Parasitology, School of Medicine, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil. FAU - Katla, Ramesh AU - Katla R AD - Laboratory of Chemistry, School of Chemistry and Food, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil. FAU - Katla, Rakhi AU - Katla R AD - Laboratory of Chemistry, School of Chemistry and Food, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil. FAU - Rosa, Gilber Ricardo AU - Rosa GR AD - Laboratory of Chemistry, School of Chemistry and Food, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil. FAU - Rosa, Clarissa Helena AU - Rosa CH AD - Laboratory of Chemistry, School of Chemistry and Food, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil. FAU - Ramos, Daniela Fernandes AU - Ramos DF AD - Laboratory for the Development of New Drugs, School of Medicine, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil. FAU - Scaini, Carlos James AU - Scaini CJ AD - Laboratory of Parasitology, School of Medicine, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil. FAU - Rodrigues, Debora Carvalho AU - Rodrigues DC AD - Laboratory for the Development of New Drugs, School of Medicine, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil. FAU - de Avila, Luciana Farias da Costa AU - de Avila LFDC AD - Laboratory of Parasitology, School of Medicine, Federal University of Rio Grande (FURG), Rio Grande, RS, Brazil. LA - eng PT - Journal Article DEP - 20261026 PL - Netherlands TA - Vet Parasitol JT - Veterinary parasitology JID - 7602745 SB - IM OTO - NOTNLM OT - Anthelmintics OT - Murine model OT - Toxocariasis COIS- Declaration of Competing Interest The authors declare that they have no competing interests. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:12 PHST- 2026/07/29 00:00 [received] PHST- 2026/10/22 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:12 [entrez] AID - S0304-4017(26)00285-2 [pii] AID - 10.1016/j.vetpar.2026.110966 [doi] PST - aheadofprint SO - Vet Parasitol. 2026 Oct 26;349:110966. doi: 10.1016/j.vetpar.2026.110966. PMID- 42950749 OWN - NLM STAT- Publisher LR - 20261028 IS - 1872-7123 (Electronic) IS - 0165-1781 (Linking) VI - 367 DP - 2026 Oct 24 TI - Machine learning and network analysis of psychosocial factors associated with non-suicidal self-injury severity among college students. PG - 117541 LID - S0165-1781(26)00600-1 [pii] LID - 10.1016/j.psychres.2026.117541 [doi] AB - BACKGROUND: Non-suicidal self-injury (NSSI) is a prevalent and clinically significant behavior. Although multiple psychosocial factors are linked to NSSI, their relative predictive importance and conditional structure remain unclear. This study applied complementary machine learning and network analysis approaches to examine psychosocial correlates of NSSI severity. METHODS: Participants were 2436 college students in China (27.1% male; mean age = 19.77 years). Psychosocial variables included emotion dysregulation, impulsivity, parenting style, negative life events, perceived social support, and emotion regulation strategies. Five supervised machine learning models were compared for predicting NSSI severity, and Shapley Additive Explanations (SHAP) analysis characterized predictor contributions. EBICglasso network analysis characterized psychosocial network structure and subgroup differences by NSSI status and sex. RESULTS: Regularized linear models performed most consistently across validation and test sets, and Elastic Net was selected for stable predictive performance and robustness to correlated predictors (RMSE = 5.33, R(2) = 0.18). Feature importance and SHAP analyses identified emotion dysregulation, maltreatment experience, suicide exposure, negative life events, and parenting-related factors as important features associated with NSSI severity, whereas perceived social support showed an inverse predictive pattern. Network analysis revealed a broadly modular partial-correlation structure, with emotion dysregulation showing relatively high strength centrality. Network Comparison Tests indicated significant differences by NSSI status and exploratory sex-related differences in overall network structure. CONCLUSIONS: Both approaches highlighted emotion dysregulation and negative life events as psychosocial correlates of NSSI severity. These findings support the complementary value of predictive and network approaches for characterizing NSSI-related psychosocial vulnerability and generating hypotheses for longitudinal research. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Liu, Hanmei AU - Liu H AD - Key Laboratory of Adolescent Cyberpsychology and Behavior (Ministry of Education), School of Psychology, Central China Normal University, Wuhan, 430079, China; Center of Health Administration and Development Studies, School of Mental Health and Psychological Sciences, Hubei University of Medicine, Shiyan, 442000, China. FAU - Guo, Yunxiao AU - Guo Y AD - Key Laboratory of Adolescent Cyberpsychology and Behavior (Ministry of Education), School of Psychology, Central China Normal University, Wuhan, 430079, China. FAU - Hu, Jiawei AU - Hu J AD - Key Laboratory of Adolescent Cyberpsychology and Behavior (Ministry of Education), School of Psychology, Central China Normal University, Wuhan, 430079, China. FAU - Xue, Dongdong AU - Xue D AD - Key Laboratory of Adolescent Cyberpsychology and Behavior (Ministry of Education), School of Psychology, Central China Normal University, Wuhan, 430079, China. FAU - Xie, Guangjin AU - Xie G AD - Key Laboratory of Adolescent Cyberpsychology and Behavior (Ministry of Education), School of Psychology, Central China Normal University, Wuhan, 430079, China; School of Teacher Education, Zunyi Normal College, Zunyi, 563006, China. FAU - Mao, Chang AU - Mao C AD - Center of Health Administration and Development Studies, School of Mental Health and Psychological Sciences, Hubei University of Medicine, Shiyan, 442000, China. FAU - Ren, Zhihong AU - Ren Z AD - Key Laboratory of Adolescent Cyberpsychology and Behavior (Ministry of Education), School of Psychology, Central China Normal University, Wuhan, 430079, China; School of Psychology, Liaoning Normal University, Dalian, 116029, China. Electronic address: ren@ccnu.edu.cn. LA - eng PT - Journal Article DEP - 20261024 PL - Ireland TA - Psychiatry Res JT - Psychiatry research JID - 7911385 SB - IM OTO - NOTNLM OT - College students OT - Emotion dysregulation OT - Machine learning OT - Network analysis OT - Non-suicidal self-injury COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:12 PHST- 2026/06/09 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:12 [entrez] AID - S0165-1781(26)00600-1 [pii] AID - 10.1016/j.psychres.2026.117541 [doi] PST - aheadofprint SO - Psychiatry Res. 2026 Oct 24;367:117541. doi: 10.1016/j.psychres.2026.117541. PMID- 42950739 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-6297 (Electronic) IS - 0001-6918 (Linking) VI - 271 DP - 2026 Oct 28 TI - Words under the skin: Physiological responses to interoceptive metaphors. PG - 107958 LID - S0001-6918(26)01759-2 [pii] LID - 10.1016/j.actpsy.2026.107958 [doi] AB - Embodied accounts of language propose that linguistic meaning is grounded in sensorimotor systems. While previous research has focused on the central nervous system, the involvement of the autonomic nervous system (ANS) in language comprehension remains underexplored. To fill this gap, we investigated whether processing interoceptive metaphors, namely figurative expressions referring to internal bodily sensations (e.g., 'I feel butterflies in my stomach'), modulates the ANS. Forty-five healthy participants read interoceptive metaphors and non-bodily-related literal sentences while cardiac, electrodermal, and respiratory signals were continuously recorded. We further distinguished between two types of interoceptive metaphors, depending on whether the interoceptive element served as the target (INT-target, e.g., 'I feel butterflies in my stomach') or source domain (INT-source, e.g., 'I thirst for success' to convey ambition) of the metaphor. Results revealed subtle but measurable effects: compared to non-bodily-related literal sentences, interoceptive metaphors were associated with higher heart rate variability and longer inspiratory duration. No significant differences emerged between INT-target and INT-source metaphors. These findings provide the first evidence that language grounded in internal bodily experience is associated with subtle differences in selected autonomic indices relative to non-bodily-related literal sentences. CI - Copyright (c) 2026 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Montalti, Martina AU - Montalti M AD - Department of Ancient and Modern Civilizations, University of Messina, Messina, Italy. FAU - Cazzato, Valentina AU - Cazzato V AD - Department of Cognitive Sciences, Psychology, Education and Cultural Studies, University of Messina, Messina, Italy; School of Psychology, Faculty of Health, Liverpool John Moores University, Liverpool, United Kingdom. FAU - Cuccio, Valentina AU - Cuccio V AD - Department of Ancient and Modern Civilizations, University of Messina, Messina, Italy. Electronic address: vcuccio@unime.it. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Acta Psychol (Amst) JT - Acta psychologica JID - 0370366 SB - IM OTO - NOTNLM OT - Autonomic nervous system OT - Electrodermal activity OT - Embodied language processing OT - Heart rate variability OT - Interoceptive metaphors OT - Physiological responses OT - Respiration COIS- Declaration of competing interest The authors declare no competing interests. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:11 PHST- 2026/07/01 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:11 [entrez] AID - S0001-6918(26)01759-2 [pii] AID - 10.1016/j.actpsy.2026.107958 [doi] PST - aheadofprint SO - Acta Psychol (Amst). 2026 Oct 28;271:107958. doi: 10.1016/j.actpsy.2026.107958. PMID- 42950730 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-3557 (Electronic) IS - 1386-1425 (Linking) VI - 367 IP - Pt 2 DP - 2026 Oct 23 TI - Exploring dual catalytic functionality of MnO(2) nanozymes through coupled colorimetric and electrochemical responses. PG - 129032 LID - S1386-1425(26)01603-3 [pii] LID - 10.1016/j.saa.2026.129032 [doi] AB - In this work, MnO(2) nanorods (MnO(2)) were synthesized and demonstrated as a versatile dual-functional nanozyme platform for integrated colorimetric and electrochemical sensing pathways. Using furazolidone (FZD) as a model probe, we unraveled a unique kinetic modulation mechanism where FZD acts as an interfacial electron-transfer modulator rather than a simple enzyme inhibitor. Spectroscopic investigations reveal that FZD selectively suppresses the over-oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to its diimine form (TMB(2+), 450 nm), promoting the accumulation of the stable radical cation (TMB(.+), 652 nm). Concurrently, the redox-active surface of MnO(2) accelerates the direct electrochemical reduction of FZD's nitro groups via a multi-electron pathway. Density functional theory (DFT) calculations confirm that the low-lying lowest unoccupied molecular orbital (LUMO) of FZD (-3.62 eV) facilitates reactive oxygen species capture, while facet-dependent adsorption energetics (E(ads) = -75.1 kJ.mol(-1) on 100) support the adsorption-controlled catalysis. Under optimized conditions, the platform achieved reliable dual-mode detection for FZD. This study provides a fundamental framework for designing single-material nanozyme systems with coupled catalytic outputs. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Mai, Chu Quynh AU - Mai CQ AD - Phenikaa University Nano Institute (PHENA), Phenikaa School of Engineering (PSE), Phenikaa University, Hanoi, 12116, Viet Nam. FAU - Mai, Vu Thi Huong AU - Mai VTH AD - Phenikaa University Nano Institute (PHENA), Phenikaa School of Engineering (PSE), Phenikaa University, Hanoi, 12116, Viet Nam. FAU - Thuc, Duong Hoang AU - Thuc DH AD - Phenikaa University Nano Institute (PHENA), Phenikaa School of Engineering (PSE), Phenikaa University, Hanoi, 12116, Viet Nam. FAU - Van Tuyen, Nguyen AU - Van Tuyen N AD - University of Transport Technology, No. 54 Trieu Khuc Street, Thanh Liet, Hanoi, 100000, Viet Nam. FAU - Thuan, Tran Hung AU - Thuan TH AD - Faculty of Agricultural Technology, VNU Univeristy of Engineering and Technology, 144 Xuan Thuy, Cau Giay, Hanoi, Viet Nam. FAU - Ngo, Xuan-Dinh AU - Ngo XD AD - Phenikaa University Nano Institute (PHENA), Phenikaa School of Engineering (PSE), Phenikaa University, Hanoi, 12116, Viet Nam. FAU - Hoang, Van-Tuan AU - Hoang VT AD - Phenikaa University Nano Institute (PHENA), Phenikaa School of Engineering (PSE), Phenikaa University, Hanoi, 12116, Viet Nam. Electronic address: tuan.hoangvan@phenikaa-uni.edu.vn. FAU - Le, Anh-Tuan AU - Le AT AD - Phenikaa University Nano Institute (PHENA), Phenikaa School of Engineering (PSE), Phenikaa University, Hanoi, 12116, Viet Nam. Electronic address: tuan.leanh@phenikaa-uni.edu.vn. LA - eng PT - Journal Article DEP - 20261023 PL - England TA - Spectrochim Acta A Mol Biomol Spectrosc JT - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JID - 9602533 SB - IM OTO - NOTNLM OT - Colorimetric sensing OT - Electrochemical sensing OT - MnO(2) OT - Nanozyme OT - Oxidase-like activity COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:11 PHST- 2026/08/18 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:11 [entrez] AID - S1386-1425(26)01603-3 [pii] AID - 10.1016/j.saa.2026.129032 [doi] PST - aheadofprint SO - Spectrochim Acta A Mol Biomol Spectrosc. 2026 Oct 23;367(Pt 2):129032. doi: 10.1016/j.saa.2026.129032. PMID- 42950729 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-3557 (Electronic) IS - 1386-1425 (Linking) VI - 367 IP - Pt 2 DP - 2026 Oct 24 TI - Reference optical characteristics of barium copper silicate pigments for non-invasive examination of painted cultural heritage. PG - 129022 LID - S1386-1425(26)01593-3 [pii] LID - 10.1016/j.saa.2026.129022 [doi] AB - Barium copper silicate pigments are representative synthetic inorganic pigments used in ancient Chinese painted objects. Their unique crystal structures and optical responses have made them important research subjects in archaeometry and cultural heritage analysis. However, barium copper silicate pigments in archaeological samples are often associated with other mineral phases, binding materials, and weathering products. Establishing reliable reference data is therefore essential for distinguishing and identifying different barium copper silicate pigments. In this study, Chinese purple (BaCuSi(2)O(6)), Chinese dark blue (BaCu(2)Si(2)O(7)), and Chinese blue (BaCuSi(4)O(10)) were synthesized by a hydrothermal method. X-ray diffraction (XRD), Raman spectroscopy, UV-Vis absorption spectroscopy, diffuse reflectance spectroscopy, steady-state photoluminescence excitation spectroscopy, and near-infrared emission spectroscopy were employed to establish comparable optical characteristic data for the three barium copper silicate pigments under identical experimental conditions. The results demonstrate that the three pigments exhibit distinguishable optical responses. The main absorption peaks were located at 578, 592, and 628 nm for Chinese purple, Chinese dark blue, and Chinese blue, respectively. Diffuse reflectance spectra revealed different reflectance behaviors among the three pigments, with Chinese blue showing the highest reflectance, followed by Chinese purple and Chinese dark blue. The corresponding photoluminescence excitation peaks were located at 581, 591, and 628 nm, while the near-infrared emission peaks appeared at 902, 917, and 954 nm, respectively. These differences indicate the influence of different crystal phases on visible-light absorption and near-infrared luminescence behaviors. Under visible-induced near-infrared luminescence imaging conditions, Chinese blue exhibited the strongest apparent luminescence response, followed by Chinese purple and Chinese dark blue. The reference pigments prepared in this study can provide materials for systematic investigations related to conservation and restoration, while the obtained spectroscopic data further enrich the optical characteristics of barium copper silicate pigments. In particular, the directly comparable reference optical data established under identical experimental conditions help address the insufficient systematic optical characterization data of barium copper silicate pigments, particularly Chinese dark blue, offering scientific support for the non-invasive identification and spatial localization of barium copper silicate pigments in painted cultural heritage objects. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Ma, Zijun AU - Ma Z AD - School of Cultural Heritage, Northwest University, Xi'an, Shaanxi 710127, China. FAU - Sun, Feng AU - Sun F AD - School of Cultural Heritage, Northwest University, Xi'an, Shaanxi 710127, China. Electronic address: sunfeng@nwu.edu.cn. FAU - Wang, Lu AU - Wang L AD - Shaanxi History Museum, Xi'an, Shaanxi 710061, China. FAU - Yan, Qingqing AU - Yan Q AD - Hubei Provincial Institute of Cultural Relics and Archaeology, Wuhan, Hubei 430071, China. FAU - Xue, Weishan AU - Xue W AD - School of Cultural Heritage, Northwest University, Xi'an, Shaanxi 710127, China. FAU - Wu, Xinru AU - Wu X AD - School of Cultural Heritage, Northwest University, Xi'an, Shaanxi 710127, China. FAU - Xu, Changzhi AU - Xu C AD - School of Cultural Heritage, Northwest University, Xi'an, Shaanxi 710127, China. LA - eng PT - Journal Article DEP - 20261024 PL - England TA - Spectrochim Acta A Mol Biomol Spectrosc JT - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JID - 9602533 SB - IM OTO - NOTNLM OT - Barium copper silicate pigments OT - Hydrothermal synthesis OT - Non-invasive identification OT - Photoluminescence OT - Visible-induced near-infrared luminescence imaging COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:11 PHST- 2026/08/08 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:11 [entrez] AID - S1386-1425(26)01593-3 [pii] AID - 10.1016/j.saa.2026.129022 [doi] PST - aheadofprint SO - Spectrochim Acta A Mol Biomol Spectrosc. 2026 Oct 24;367(Pt 2):129022. doi: 10.1016/j.saa.2026.129022. PMID- 42950727 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-3557 (Electronic) IS - 1386-1425 (Linking) VI - 367 IP - Pt 2 DP - 2026 Oct 23 TI - A self-assembled silver nanowire-based SERS sensor with creatinine internal standard for the detection of uric acid in urine. PG - 129030 LID - S1386-1425(26)01601-X [pii] LID - 10.1016/j.saa.2026.129030 [doi] AB - Rapid and reliable detection of uric acid is crucial for a prompt diagnosis of metabolic disorders. However, this remains challenging due to the intricacy of biological matrices and the limitations of current analytical methods. In this study, a surface-enhanced Raman scattering (SERS) sensor based on silver nanowire arrays functionalized with cetyltrimethylammonium chloride (Ag NWs@CTAC) was developed for the quantitative detection of uric acid in human urine. To ensure accuracy, creatinine, a metabolite excreted at a steady rate, was introduced as an internal standard to correct for signal fluctuations. The positively charged CTAC layer enhanced selectivity by electrostatically adsorbing negatively charged uric acid. Additionally, the uniformly arranged Ag NWs@CTAC array provided high density and repeatable SERS hotspots, ensuring both uniformity and signal enhancement. The sensor achieved a detection limit as low as 1.27 mg L(-1) for uric acid, along with accurate recovery rates in artificial urine samples. These features make the Ag NWs@CTAC SERS sensor a promising candidate for quick, accurate, and reliable uric acid detection in clinical and point-of-care applications. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Jiang, Panpan AU - Jiang P AD - College of Optoelectronic Materials and Technology, Jianghan University, Wuhan, 430056, China; Institute of Intelligent Sport and Proactive Health, Department of Health and Physical Education, Jianghan University, Wuhan, 430056, China. FAU - Gao, Nan AU - Gao N AD - Institute of Intelligent Sport and Proactive Health, Department of Health and Physical Education, Jianghan University, Wuhan, 430056, China. FAU - Wu, Yuxiang AU - Wu Y AD - College of Optoelectronic Materials and Technology, Jianghan University, Wuhan, 430056, China; Institute of Intelligent Sport and Proactive Health, Department of Health and Physical Education, Jianghan University, Wuhan, 430056, China. Electronic address: yxwu@jhun.edu.cn. LA - eng PT - Journal Article DEP - 20261023 PL - England TA - Spectrochim Acta A Mol Biomol Spectrosc JT - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JID - 9602533 SB - IM OTO - NOTNLM OT - Internal standard OT - Self-assembly OT - Surface-enhanced Raman scattering OT - Uric acid COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:11 PHST- 2026/03/12 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:11 [entrez] AID - S1386-1425(26)01601-X [pii] AID - 10.1016/j.saa.2026.129030 [doi] PST - aheadofprint SO - Spectrochim Acta A Mol Biomol Spectrosc. 2026 Oct 23;367(Pt 2):129030. doi: 10.1016/j.saa.2026.129030. PMID- 42950719 OWN - NLM STAT- Publisher LR - 20261028 IS - 1095-8630 (Electronic) IS - 0301-4797 (Linking) VI - 419 DP - 2026 Oct 28 TI - Carbon emission reduction in urban green space construction: A multi-module optimization framework for multi-scale applications. PG - 131236 LID - S0301-4797(26)02696-4 [pii] LID - 10.1016/j.jenvman.2026.131236 [doi] AB - Urban green spaces serve as critical spatial carriers for mitigating the urban heat island effect and sustaining urban carbon balance. Their materialization phase spans material production, transportation, and on-site construction, and the associated carbon emissions are largely predetermined by planning and design decisions, yet no systematic optimization pathway has been established. Conventional life cycle assessment relies on static databases, while experience-driven design adjustments struggle to accommodate multiple constraints imposed by policy, functionality, and spatial distribution simultaneously. This study presents a multi-module optimization framework that sequentially links carbon emission calculation, optimization algorithms, and spatial layout adjustment and applies it to three real-world green space projects at different spatial scales. A performance comparison of six algorithms across the three project scales showed that particle swarm optimization (PSO) consistently obtained optimal or near-optimal solutions and was therefore selected as the core solver. Under the engineering constraint of a maximum adjustment magnitude (MA) of 0.10, the mean emission reduction rates for the three sites were 7.34%, 8.48%, and 6.06%, respectively. Under different constraint scenarios, the framework further translates optimization outputs into implementable land-use adjustment schemes. By advancing urban green space carbon management from post-construction accounting to upstream intervention at the planning and design stage, this framework provides a quantifiable decision-support tool for carbon neutrality goals in urban green space planning. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Tao, Peiyuan AU - Tao P AD - Department of Landscape Architecture, College of Landscape Architecture, Beijing Forestry University, 35 Qinghua East Road, Haidian District, Beijing, 100083, China. Electronic address: peiyuantao@bjfu.edu.cn. FAU - Wang, Yangzi AU - Wang Y AD - Department of Landscape Architecture, College of Landscape Architecture, Beijing Forestry University, 35 Qinghua East Road, Haidian District, Beijing, 100083, China. Electronic address: yz365@bjfu.edu.cn. FAU - Shao, Ming AU - Shao M AD - Department of Landscape Architecture, College of Landscape Architecture, Beijing Forestry University, 35 Qinghua East Road, Haidian District, Beijing, 100083, China. Electronic address: shao_0701@bjfu.edu.cn. FAU - Zhang, Yichun AU - Zhang Y AD - Department of Landscape Architecture, College of Landscape Architecture, Beijing Forestry University, 35 Qinghua East Road, Haidian District, Beijing, 100083, China. Electronic address: zyc626June@bjfu.edu.cn. FAU - Zhang, Ruonan AU - Zhang R AD - Department of Landscape Architecture, College of Landscape Architecture, Beijing Forestry University, 35 Qinghua East Road, Haidian District, Beijing, 100083, China. Electronic address: zrn1123@bjfu.edu.cn. FAU - Shi, Qirong AU - Shi Q AD - Department of Landscape Architecture, College of Landscape Architecture, Beijing Forestry University, 35 Qinghua East Road, Haidian District, Beijing, 100083, China. Electronic address: S7250639@bjfu.edu.cn. FAU - Yao, Peng AU - Yao P AD - Department of Landscape Architecture, College of Landscape Architecture, Beijing Forestry University, 35 Qinghua East Road, Haidian District, Beijing, 100083, China. Electronic address: chinayp@bjfu.edu.cn. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - J Environ Manage JT - Journal of environmental management JID - 0401664 SB - IM OTO - NOTNLM OT - Carbon emission OT - Environmental decision support OT - Materialization phase OT - Optimization algorithm OT - Urban green space COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:11 PHST- 2026/04/02 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:11 [entrez] AID - S0301-4797(26)02696-4 [pii] AID - 10.1016/j.jenvman.2026.131236 [doi] PST - aheadofprint SO - J Environ Manage. 2026 Oct 28;419:131236. doi: 10.1016/j.jenvman.2026.131236. PMID- 42950703 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-0267 (Electronic) IS - 0020-1383 (Linking) VI - 57 IP - 12 DP - 2026 Oct 26 TI - Early fecal diversion versus no initial diversion in blunt open pelvic fractures: A systematic review and meta-analysis of pelvic infection and mortality. PG - 113828 LID - S0020-1383(26)00815-6 [pii] LID - 10.1016/j.injury.2026.113828 [doi] AB - BACKGROUND: The benefit of routine early fecal diversion in blunt open pelvic fractures remains uncertain. We examined its association with pelvic infection and short-term mortality. METHODS: MEDLINE/PubMed, Embase, Scopus, Web of Science Core Collection, and CENTRAL were searched from inception through 12 August 2026, with citation searching. Comparative observational studies were synthesized using crude risk ratios (RRs), REML random-effects models, modified Hartung-Knapp confidence intervals, and Mantel-Haenszel sensitivity analyses without continuity correction. Two reviewers independently selected studies, extracted data, and assessed ROBINS-I risk of bias and GRADE certainty. RESULTS: Eighteen observational comparative reports were retained. Potential cohort overlap prevented a valid unique overall participant total. Eight cohorts contributed to broad pelvic infection (171 participants; seven estimable): RR 0.78 (95% CI 0.35-1.70; I(2)=19.6%). The stricter outcome-definition analysis included five cohorts (88 participants; four estimable): RR 0.72 (95% CI 0.07-6.97). Excluding two author-reported infected hematomas through an existing replacement sensitivity gave RR 0.84 (95% CI 0.34-2.05). Eight mortality cohorts (236 participants) yielded RR 0.82 (95% CI 0.33-2.07; I(2)=19.0%). Post-2006 evidence remained inconclusive for infection (three cohorts; RR 0.74, 95% CI 0.009-58.85) and mortality (five cohorts; RR 1.19, 95% CI 0.27-5.26). Diversion was preferentially used in patients with more severe injuries. All reports had Serious or Critical risk of bias; certainty was very low. CONCLUSIONS: Available evidence does not establish a reduction in pelvic infection or short-term mortality with routine early diversion. Confounding, inconsistent outcome ascertainment, and imprecision prevent causal conclusions or exclusion of benefit in selected high-risk injuries. CI - Copyright (c) 2026 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Ferrari, Federico AU - Ferrari F AD - University of Pavia, Corso Strada Nuova 65, Pavia, 27100, Italy. Electronic address: federico.ferrari10@universitadipavia.it. FAU - Ancieta, Marioly Dahinira Herrera AU - Ancieta MDH AD - Department of Internal Medicine and Therapeutics, University of Pavia, Viale Golgi 19, Pavia, 27100, Italy. FAU - Laura, Lucero Ytala Samame AU - Laura LYS AD - Faculty of Human Medicine, Universidad de San Martin de Porres, Avenida Alameda del Corregidor, La Molina, Lima, 1531, Peru. FAU - Botti, Marta AU - Botti M AD - Department of General Surgery 2, Fondazione IRCCS Policlinico San Matteo, Viale Golgi 19, Pavia, 27100, Italy. LA - eng PT - Journal Article DEP - 20261026 PL - Netherlands TA - Injury JT - Injury JID - 0226040 SB - IM OTO - NOTNLM OT - Colostomy OT - Fecal diversion OT - Meta-analysis OT - Open pelvic fracture OT - Pelvic sepsis OT - Trauma COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:11 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:11 [entrez] AID - S0020-1383(26)00815-6 [pii] AID - 10.1016/j.injury.2026.113828 [doi] PST - aheadofprint SO - Injury. 2026 Oct 26;57(12):113828. doi: 10.1016/j.injury.2026.113828. PMID- 42950697 OWN - NLM STAT- Publisher LR - 20261028 IS - 1878-5891 (Electronic) IS - 0378-5955 (Linking) VI - 483 DP - 2026 Oct 23 TI - Development of a noninvasive video-oculography setup for determining VOR presence in a severe vestibular impairment mouse model. PG - 109844 LID - S0378-5955(26)00315-1 [pii] LID - 10.1016/j.heares.2026.109844 [doi] AB - The vestibulo-ocular reflex (VOR), which stabilizes gaze during head movement, serves as a key indicator of vestibular integrity. Conventional systems for VOR measurement in mice often require invasive head fixation, posing risks to animal welfare and experimental reproducibility. In this study, we developed and validated a noninvasive immobilization setup for detecting the presence of VOR responses in a severe vestibular impairment mouse model, offering a welfare-oriented alternative to surgically fixed systems. Using video-oculography (VOG), we assessed VOR responses in control and vestibular-impaired CD-1 mice, with vestibular dysfunction induced via oral administration of the ototoxic compound allylnitrile. As expected, allylnitrile-treated mice exhibited a near-complete absence of VOR responses, confirming profound vestibular impairment. Complementary assessments included Vestibular Dysfunction Index (VDI) scoring, swimming tests, immunofluorescent staining for hair cell counts in vestibular cristae and hearing tests. Because the induced, severe deficits precluded quantitative gain and phase analysis in this study, the set-up is currently limited to binary VOR detection in this model. However, further research will determine if under conditions of partial or progressive impairment, this noninvasive system supports more detailed characterization of VOR dynamics. Overall, the optimized, surgery-free setup demonstrated here enhances the reproducibility and welfare standards of vestibular testing in mice, offering a refined platform for detecting VOR presence in a severe vestibular impairment mouse model. CI - Copyright (c) 2026 The Author(s). Published by Elsevier B.V. All rights reserved. FAU - Aben, Fien AU - Aben F AD - Department of Translational Neurosciences, Faculty of Medicine and Health Sciences, University of Antwerp, Universiteitsplein 1, Wilrijk, 2610, Belgium. Electronic address: fien.aben@uantwerpen.be. FAU - Bossaerts, Lien AU - Bossaerts L AD - Department of Translational Neurosciences, Faculty of Medicine and Health Sciences, University of Antwerp, Universiteitsplein 1, Wilrijk, 2610, Belgium. Electronic address: lienbossaerts@hotmail.com. FAU - Keteleer, Geert AU - Keteleer G AD - Department of Translational Neurosciences, Faculty of Medicine and Health Sciences, University of Antwerp, Universiteitsplein 1, Wilrijk, 2610, Belgium. Electronic address: geert.keteleer@uantwerpen.be. FAU - Palou, Aida AU - Palou A AD - Departament de Ciencies Fisiologiques, Universitat de Barcelona, Carrer de la Feixa Llarga, s/n, 08907 l'Hospitalet de Llobergat, Barcelona, Spain; Institut de Neurociences, Universitat de Barcelona, Edif. de Ponent, Pg Vall d'Hebron, 171, Horta-Guinardo, Barcelona, 08035, Spain; Institut d'Investigacio Biomedica de Bellvitge (IDIBELL), Avinguda de la Granvia de l'Hospitalet 199, 08908 l'Hospitalet de Llobregat, Barcelona, Spain. Electronic address: apaloumi45@ub.edu. FAU - Renner, Ana AU - Renner A AD - Departament de Ciencies Fisiologiques, Universitat de Barcelona, Carrer de la Feixa Llarga, s/n, 08907 l'Hospitalet de Llobergat, Barcelona, Spain; Institut de Neurociences, Universitat de Barcelona, Edif. de Ponent, Pg Vall d'Hebron, 171, Horta-Guinardo, Barcelona, 08035, Spain; Institut d'Investigacio Biomedica de Bellvitge (IDIBELL), Avinguda de la Granvia de l'Hospitalet 199, 08908 l'Hospitalet de Llobregat, Barcelona, Spain. Electronic address: ana.renner@ub.edu. FAU - Llorens, Jordi AU - Llorens J AD - Departament de Ciencies Fisiologiques, Universitat de Barcelona, Carrer de la Feixa Llarga, s/n, 08907 l'Hospitalet de Llobergat, Barcelona, Spain; Institut de Neurociences, Universitat de Barcelona, Edif. de Ponent, Pg Vall d'Hebron, 171, Horta-Guinardo, Barcelona, 08035, Spain; Institut d'Investigacio Biomedica de Bellvitge (IDIBELL), Avinguda de la Granvia de l'Hospitalet 199, 08908 l'Hospitalet de Llobregat, Barcelona, Spain. Electronic address: jllorens@ub.edu. FAU - Szewczyk, Krystyna AU - Szewczyk K AD - Department of Translational Neurosciences, Faculty of Medicine and Health Sciences, University of Antwerp, Universiteitsplein 1, Wilrijk, 2610, Belgium. Electronic address: krystyna.szewczyk@uantwerpen.be. FAU - Verdoodt, Dorien AU - Verdoodt D AD - Department of Translational Neurosciences, Faculty of Medicine and Health Sciences, University of Antwerp, Universiteitsplein 1, Wilrijk, 2610, Belgium; Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Universiteitsplein 1, Wilrijk, 2610, Belgium. Electronic address: Dorien.Verdoodt@uantwerpen.be. FAU - Van Rompaey, Vincent AU - Van Rompaey V AD - Department of Translational Neurosciences, Faculty of Medicine and Health Sciences, University of Antwerp, Universiteitsplein 1, Wilrijk, 2610, Belgium; Department of Otorhinolaryngology and Head & Neck Surgery, Antwerp University Hospital, Drie Eikenstraat 655, Edegem, 2650, Belgium. Electronic address: vincent.vanrompaey@uantwerpen.be. LA - eng PT - Journal Article DEP - 20261023 PL - Netherlands TA - Hear Res JT - Hearing research JID - 7900445 SB - IM OTO - NOTNLM OT - Animal welfare OT - Ototoxicity OT - Vestibular dysfunction OT - Vestibulo-ocular reflex OT - Video-oculography COIS- Declaration of conflicting interest The authors declare no competing interests. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:11 PHST- 2026/08/04 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:11 [entrez] AID - S0378-5955(26)00315-1 [pii] AID - 10.1016/j.heares.2026.109844 [doi] PST - aheadofprint SO - Hear Res. 2026 Oct 23;483:109844. doi: 10.1016/j.heares.2026.109844. PMID- 42950695 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-0402 (Electronic) IS - 1367-5931 (Linking) VI - 95 DP - 2026 Oct 28 TI - FBXO22 is a promising E3 ubiquitin ligase for targeted protein degradation. PG - 102803 LID - S1367-5931(26)00152-3 [pii] LID - 10.1016/j.cbpa.2026.102803 [doi] AB - Targeted protein degradation (TPD) is an exciting new strategy to destroy, rather than simply inhibit, therapeutic targets of interest. Degrader molecules function by recruiting protein targets to E3 ubiquitin ligases, resulting in target polyubiquitination and destruction. Although there are >600 E3s, relatively few, notably CRBN and VHL, are routinely used for TPD applications in either academic or clinical settings. An up-and-coming ligase on the TPD scene is FBXO22, which has multiple distinct features when compared to other commonly used E3s. Here, we highlight the native functions of FBXO22 and the chemical matter (degraders) used to repurpose FBXO22's ubiquitination activity. As FBXO22 is overexpressed in a variety of cancers, this may provide a therapeutic window to destroy targets selectively in cancer cells. Taken together, FBXO22 fulfills a necessary void in the TPD field and provides a new approach to develop novel cancer therapeutics. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved. FAU - Liu, Tongkun AU - Liu T AD - Center for Integrative Chemical Biology and Drug Discovery, Division of Chemical Biology and Medicinal Chemistry, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. FAU - Robertson, Kevin C AU - Robertson KC AD - Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599, USA; Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA. FAU - McGowan, Amanda S AU - McGowan AS AD - Center for Integrative Chemical Biology and Drug Discovery, Division of Chemical Biology and Medicinal Chemistry, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. FAU - Thompson, Reagan E AU - Thompson RE AD - Department of Chemistry, University of North Carolina, Chapel Hill, NC 27599, USA. FAU - James, Lindsey I AU - James LI AD - Center for Integrative Chemical Biology and Drug Discovery, Division of Chemical Biology and Medicinal Chemistry, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA; Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA. Electronic address: ingerman@email.unc.edu. FAU - Brown, Nicholas G AU - Brown NG AD - Department of Pharmacology, University of North Carolina, Chapel Hill, NC 27599, USA; Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599, USA. Electronic address: nbrown1@med.unc.edu. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - Curr Opin Chem Biol JT - Current opinion in chemical biology JID - 9811312 SB - IM COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Nicholas Brown reports financial support was provided by Amgen Inc. The other authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:10 PHST- 2026/07/31 00:00 [received] PHST- 2026/09/10 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:10 [entrez] AID - S1367-5931(26)00152-3 [pii] AID - 10.1016/j.cbpa.2026.102803 [doi] PST - aheadofprint SO - Curr Opin Chem Biol. 2026 Oct 28;95:102803. doi: 10.1016/j.cbpa.2026.102803. PMID- 42950688 OWN - NLM STAT- Publisher LR - 20261028 IS - 1872-7131 (Electronic) IS - 0387-7604 (Linking) VI - 48 IP - 6 DP - 2026 Oct 28 TI - EEG network analysis to identify cortical pathology in Lennox-Gastaut syndrome. PG - 104597 LID - S0387-7604(26)00098-7 [pii] LID - 10.1016/j.braindev.2026.104597 [doi] AB - OBJECTIVE: This study aimed to identify preoperative electrophysiologic biomarkers to localize the epileptogenic zone (EZ) and predict surgical outcomes in patients with Lennox-Gastaut syndrome (LGS) using a graph-theoretical network analysis. METHODS: We retrospectively analyzed preoperative long-term EEG data from 71 LGS patients in three subgroups: Group A (n = 23), with favorable outcomes after resective surgery (RS); Group B (n = 22), with unfavorable outcomes after RS; and Group C (n = 26), who underwent palliative surgery instead of RS. We analyzed 500-ms EEG windows centered on the point of peak phase coupling preceding interictal generalized sharp-and-slow waves. We computed two sets of biomarkers: (1) the clustering coefficient (CCE) to assess overall network connectivity, and (2) a novel 'Network Dominance Score' (the product of Betweenness Centrality, Node Out-Degree, and Node Out-Strength) to identify the putative ictogenic hub. RESULTS: The CCE effectively differentiate the favorable-outcome group (Group A), the unfavorable-outcome group (Group B), and the palliative surgery group (Group C) (Area Under the Curve = 0.915). The Network Dominance Score achieved 100% accuracy in correctly lateralizing the lesion for all patients in the Group A. Conversely, for the Group B, this analysis identified either bilateral or incorrectly localized hubs, demonstrating a clear correlation between our analytical findings and surgical outcomes. CONCLUSION: This graph-theoretical approach offers a promising non-invasive tool to augment conventional presurgical evaluations, improve patient selection for RS, and predict surgical outcomes in individuals with LGS. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Wang, Zhi Ji AU - Wang ZJ AD - Division of Pediatric Neurology, Department of Pediatrics, Severance Children's Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea; Radio Frequency Bio Research Center, Kwangwoon University, Seoul, Republic of Korea. FAU - Kwon, Hye Eun AU - Kwon HE AD - Department of Pediatrics, International St. Mary's Hospital, Catholic Kwandong University College of Medicine, Incheon, Republic of Korea. FAU - Shin, Hui Jin AU - Shin HJ AD - Division of Pediatric Neurology, Department of Pediatrics, Severance Children's Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea. FAU - Lee, Seok Jin AU - Lee SJ AD - Division of Pediatric Neurology, Department of Pediatrics, Severance Children's Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea. FAU - Hur, Yun Jung AU - Hur YJ AD - Department of Pediatrics, Haeundae Paik Hospital, Inje University College of Medicine, Busan, Republic of Korea. FAU - Yang, Shan AU - Yang S AD - Radio Frequency Bio Research Center, Kwangwoon University, Seoul, Republic of Korea. FAU - Enkhzaya, Ganbold AU - Enkhzaya G AD - Radio Frequency Bio Research Center, Kwangwoon University, Seoul, Republic of Korea. FAU - Kim, Nam Young AU - Kim NY AD - Radio Frequency Bio Research Center, Kwangwoon University, Seoul, Republic of Korea; Laboratory of Molecular Pathology and Cancer Genomics, Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, Seoul, Republic of Korea. Electronic address: nykim@kw.ac.kr. FAU - Kim, Eun Seong AU - Kim ES AD - Radio Frequency Bio Research Center, Kwangwoon University, Seoul, Republic of Korea. Electronic address: 3037eskim@gmail.com. FAU - Kim, Heung Dong AU - Kim HD AD - Department of Pediatrics, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea. Electronic address: hdkimmd@yuhs.ac.kr. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Brain Dev JT - Brain & development JID - 7909235 SB - IM OTO - NOTNLM OT - Electroencephalography OT - Epileptogenic zone OT - Graph theory OT - Lennox-Gastaut syndrome OT - Surgical outcome prediction COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:10 PHST- 2026/05/21 00:00 [received] PHST- 2026/08/20 00:00 [revised] PHST- 2026/09/08 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:10 [entrez] AID - S0387-7604(26)00098-7 [pii] AID - 10.1016/j.braindev.2026.104597 [doi] PST - aheadofprint SO - Brain Dev. 2026 Oct 28;48(6):104597. doi: 10.1016/j.braindev.2026.104597. PMID- 42950683 OWN - NLM STAT- Publisher LR - 20261028 IS - 1768-3254 (Electronic) IS - 0223-5234 (Linking) VI - 321 IP - Pt 3 DP - 2026 Oct 23 TI - H(2)S-activated NO-releasing theranostics for combined gas and photodynamic therapy against colorectal cancer. PG - 119450 LID - S0223-5234(26)00895-0 [pii] LID - 10.1016/j.ejmech.2026.119450 [doi] AB - NO gas therapy has been constrained by its poor targeting and insufficient cytotoxicity toward tumor tissues. Due to hydrogen sulfide (H(2)S) overexpression in colorectal cancer, combining H(2)S-activated and light-controlled NO release with other phototherapeutic modalities enabled precise delivery of NO with high spatiotemporal accuracy and negligible drug resistance, while enhancing therapeutic efficacy. In this study, we developed H(2)S-activated theranostics BSO-NO for combined NO gas and photodynamic therapy against colorectal cancer, guided by H(2)S-activated fluorescence enhancement. BSO-NO selectively reacted with the overexpressed H(2)S in colorectal cancer to generate BOH-NO, accompanied by an enhancement of fluorescence signal. Subsequent irradiation was directed at the tumor site to trigger on-demand release of NO and reactive oxygen species (ROS) from BOH-NO. Notably, H(2)S overexpressed in colorectal tumors also promoted NO release, thereby boosting therapeutic efficacy of NO gas therapy. This combined gas and photodynamic therapy further exhibited excellent antitumor ablation performance in vivo. CI - Copyright (c) 2026 Elsevier Masson SAS. All rights reserved. FAU - Wu, Kehuan AU - Wu K AD - Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Fudan University, Shanghai, 201301, China. FAU - Ma, Shaozong AU - Ma S AD - Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Fudan University, Shanghai, 201301, China. FAU - Liu, Chenjia AU - Liu C AD - Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Fudan University, Shanghai, 201301, China. FAU - Zhao, Mengting AU - Zhao M AD - Key Laboratory for Advanced Materials and Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China. FAU - Xue, Yongxing AU - Xue Y AD - Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Fudan University, Shanghai, 201301, China. FAU - Cheng, Jiaxin AU - Cheng J AD - Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Fudan University, Shanghai, 201301, China. FAU - Zhao, Chunchang AU - Zhao C AD - Key Laboratory for Advanced Materials and Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, China. FAU - Sun, Lixin AU - Sun L AD - Shandong Provincial Key Laboratory of Brain Science, Institute of Brain Science and Brain-inspired Research, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, 250117, China; Shandong Institute of Brain Science and Brain-inspired Research, Jinan, 250117, China. Electronic address: lixin_sun@sdfmu.edu.cn. FAU - Gu, Xianfeng AU - Gu X AD - Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Fudan University, Shanghai, 201301, China. Electronic address: xfgu@fudan.edu.cn. LA - eng PT - Journal Article DEP - 20261023 PL - France TA - Eur J Med Chem JT - European journal of medicinal chemistry JID - 0420510 SB - IM OTO - NOTNLM OT - Colorectal cancer OT - H(2)S-Activated OT - NO gas therapy OT - Photodynamic therapy OT - Theranostics COIS- Declaration of competing interest The authors that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:10 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:10 [entrez] AID - S0223-5234(26)00895-0 [pii] AID - 10.1016/j.ejmech.2026.119450 [doi] PST - aheadofprint SO - Eur J Med Chem. 2026 Oct 23;321(Pt 3):119450. doi: 10.1016/j.ejmech.2026.119450. PMID- 42950681 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-3336 (Electronic) IS - 0304-3894 (Linking) VI - 518 DP - 2026 Oct 23 TI - Chiral antibiotics amplify the phage-associated antibiotic resistome in salinized soil. PG - 144003 LID - S0304-3894(26)02984-5 [pii] LID - 10.1016/j.jhazmat.2026.144003 [doi] AB - Anthropogenic global change is intensifying antibiotic pollution and soil salinization, yet how salinity shapes bacteria-phage interactions to drive the dissemination of antibiotic resistance genes (ARGs) under chiral antibiotic pressure remains poorly understood. Using viral metagenomic analyses and laboratory validation, we show that salinity amplifies the enrichment of total and high-risk ARGs along gradients of racemic ofloxacin and its S-enantiomer levofloxacin, concomitant with the parallel accumulation of mobile genetic elements and virulence factors. High-concentration antibiotic treatment in saline soils selected for stress-tolerant, fast-growing antibiotic-resistant bacteria (ARBs) carrying abundant prophages and diverse defense systems, thereby promoting tight ARB-phage interactions. Under these dual extreme stresses, lysogenic phages were enriched, enhancing their carriage of ARGs, auxiliary metabolic genes (AMGs) that bolster host survival, and antidefense genes via a "piggyback-the-winner" strategy, thereby increasing ARG dissemination potential. Notably, high-concentration levofloxacin exerted stronger selective pressure than ofloxacin, increasing virus-to-host ratios and phage-associated ARG accumulation, as supported by incubation assays. Collectively, our findings support a mechanistic framework in which soil salinization interacts with chiral antibiotic exposure, particularly levofloxacin, to increase phage-associated ARG dissemination potential. This highlights a long-underappreciated, Anthropocene-driven pathway intensifying environmental resistance risks and posing new challenges for the One Health framework. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Xie, Ting AU - Xie T AD - State Key Laboratory of Regional and Urban Ecology, Ningbo Observation and Research Station, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; Zhejiang Key Laboratory of Pollution Control for Port-Petrochemical Industry, CAS Haixi Industrial Technology Innovation Center in Beilun, Ningbo 315830, China. FAU - Lin, Da AU - Lin D AD - State Key Laboratory of Regional and Urban Ecology, Ningbo Observation and Research Station, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; Zhejiang Key Laboratory of Pollution Control for Port-Petrochemical Industry, CAS Haixi Industrial Technology Innovation Center in Beilun, Ningbo 315830, China. FAU - Tong, Si-Qi AU - Tong SQ AD - Key Laboratory of Marine Environment and Ecology, Ministry of Education and College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China. FAU - Wang, Qi AU - Wang Q AD - Key Laboratory of Marine Environment and Ecology, Ministry of Education and College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China. FAU - Ni, Ni AU - Ni N AD - Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing Institute of Environmental Science, Ministry of Ecology and Environment of the People's Republic of China, Nanjing 210042, China. FAU - Wang, Na AU - Wang N AD - Key Laboratory of Pesticide Environmental Assessment and Pollution Control, Nanjing Institute of Environmental Science, Ministry of Ecology and Environment of the People's Republic of China, Nanjing 210042, China. FAU - Yang, Zhugen AU - Yang Z AD - Faculty of Engineering and Applied Sciences, Cranfield University, Milton Keynes MK43 0AL, UK. FAU - Liu, Xiao-Hui AU - Liu XH AD - Key Laboratory of Marine Environment and Ecology, Ministry of Education and College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China. Electronic address: lxh7786@ouc.edu.cn. FAU - Zhu, Dong AU - Zhu D AD - State Key Laboratory of Regional and Urban Ecology, Ningbo Observation and Research Station, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; Zhejiang Key Laboratory of Pollution Control for Port-Petrochemical Industry, CAS Haixi Industrial Technology Innovation Center in Beilun, Ningbo 315830, China. Electronic address: dzhu@iue.ac.cn. LA - eng PT - Journal Article DEP - 20261023 PL - Netherlands TA - J Hazard Mater JT - Journal of hazardous materials JID - 9422688 SB - IM OTO - NOTNLM OT - Antibiotic resistance genes (ARGs) OT - Auxiliary metabolic genes (AMGs) OT - Chiral antibiotic OT - Life history traits OT - Phage-host interaction COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:10 PHST- 2026/06/24 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:10 [entrez] AID - S0304-3894(26)02984-5 [pii] AID - 10.1016/j.jhazmat.2026.144003 [doi] PST - aheadofprint SO - J Hazard Mater. 2026 Oct 23;518:144003. doi: 10.1016/j.jhazmat.2026.144003. PMID- 42950667 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-3336 (Electronic) IS - 0304-3894 (Linking) VI - 518 DP - 2026 Oct 23 TI - Facilitating the sequential conversion of *NO(x) intermediates by tandem catalyst and electrolyte engineering for high ammonia selectivity from electrochemical nitrate reduction. PG - 144016 LID - S0304-3894(26)02997-3 [pii] LID - 10.1016/j.jhazmat.2026.144016 [doi] AB - Efficient electrochemical nitrate reduction to ammonia (eNO(3)RA) requires the continuous conversion of surface-bound nitrate reduction intermediates, especially at low nitrate concentrations. We construct a tandem catalyst by electrodeposition of NiO(x) on the Cu(x)O nanowires to promote the sequential conversion of *NO(2) to *NO, and then a superior performance with 95.89% nitrate conversion and 95.83% ammonia selectivity is achieved for 1 mM nitrate. In situ Raman spectroscopy and density functional theory calculations confirm that the NiO(x) promotes the conversion of *NO(2) to *NO by lowering the corresponding free energy barrier from 0.85 to 0.59 eV. Additionally, the enrichment of potassium ions in the electric double layer can alleviate the electrostatic repulsion at the electrode-electrolyte interface, which facilitates the accumulation of nitrate and stimulates the eNO(3)RA at a low concentration of nitrate. Our results provide a deep insight into the eNO(3)RA pathways at low concentrations and may facilitate their application towards dilute nitrate waste streams. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Zhang, Kemeng AU - Zhang K AD - School of Ecology and Environment, Zhengzhou University, Zhengzhou, Henan 450001, PR China. FAU - Li, Fang AU - Li F AD - School of Ecology and Environment, Zhengzhou University, Zhengzhou, Henan 450001, PR China. FAU - Guo, Qilong AU - Guo Q AD - School of Ecology and Environment, Zhengzhou University, Zhengzhou, Henan 450001, PR China. FAU - Zhang, Changsen AU - Zhang C AD - School of Ecology and Environment, Zhengzhou University, Zhengzhou, Henan 450001, PR China. FAU - Xu, Qixiang AU - Xu Q AD - School of Ecology and Environment, Zhengzhou University, Zhengzhou, Henan 450001, PR China. FAU - He, Yunfei AU - He Y AD - Zhejiang Provincial Key Laboratory of Wetland Intelligent Monitoring and Ecological Restoration, School of Engineering, Hangzhou Normal University, Hangzhou 310018, PR China. Electronic address: heyyff@hznu.edu.cn. FAU - Liu, Panpan AU - Liu P AD - School of Ecology and Environment, Zhengzhou University, Zhengzhou, Henan 450001, PR China. Electronic address: liupanpan@zzu.edu.cn. LA - eng PT - Journal Article DEP - 20261023 PL - Netherlands TA - J Hazard Mater JT - Journal of hazardous materials JID - 9422688 SB - IM OTO - NOTNLM OT - Electrocatalytic nitrate reduction to ammonia OT - Low concentration, Electrical double layer OT - Tandem catalyst, Electrolyte engineering COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:10 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:10 [entrez] AID - S0304-3894(26)02997-3 [pii] AID - 10.1016/j.jhazmat.2026.144016 [doi] PST - aheadofprint SO - J Hazard Mater. 2026 Oct 23;518:144016. doi: 10.1016/j.jhazmat.2026.144016. PMID- 42950666 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-3336 (Electronic) IS - 0304-3894 (Linking) VI - 518 DP - 2026 Oct 27 TI - Old meets new: How emerging contaminants reshape the environmental behavior of legacy contaminants in soils-interaction mechanisms, remediation reassessment, and the regulatory role of soil organic matter. PG - 144041 LID - S0304-3894(26)03022-0 [pii] LID - 10.1016/j.jhazmat.2026.144041 [doi] AB - Microplastics, per- and polyfluoroalkyl substances (PFAS), and antibiotics coexist in soils with legacy metal(loid)s, polycyclic aromatic hydrocarbons (PAHs), and pesticides. These mixtures can alter contaminant fate, microbial selection, and remediation performance, but direct soil evidence remains uneven. This review synthesizes studies mainly from 2015 to 2025, earlier work, and selected early-online papers through June 2026. Soil evidence supports microplastic interactions with metals and PAHs; PFAS-metal evidence remains limited, mainly to retention. Antibiotic-metal evidence primarily concerns resistance co-selection rather than altered metal fate. We organize candidate processes into overlapping diagnostic tiers (physical co-location, interfacial chemical coupling, and biologically mediated feedback) and appraise evidence by study design, environmental relevance, mechanistic support, and risk of bias. A comparative map specifies required observations, alternative explanations, endpoint direction, and direct-evidence confidence. Concentration addition, independent action, and the combination index apply only to commensurable toxicological dose-response endpoints; transport, speciation, accumulation, and resistance require factorial or response-surface analyses. HIX, SUVA254, and PARAFAC components remain conditional dissolved organic matter proxies requiring method controls and independent validation. We classify remediation conclusions as direct mixture evidence, transferred single-contaminant principles, or author recommendations. The synthesis separates established findings from provisional interpretations. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Song, Jinhang AU - Song J AD - Beijing Key Laboratory of Water Resources & Environmental Engineering, China University of Geosciences, Beijing 100083, China; State Key Laboratory of Environmental Criteria and Risk Assessment, and Key Laboratory of Groundwater Pollution Simulation and Control of Ministry of Ecology and Environment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. FAU - Zheng, Jianguo AU - Zheng J AD - State Key Laboratory of Environmental Criteria and Risk Assessment, and Key Laboratory of Groundwater Pollution Simulation and Control of Ministry of Ecology and Environment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. FAU - Liu, Fei AU - Liu F AD - Beijing Key Laboratory of Water Resources & Environmental Engineering, China University of Geosciences, Beijing 100083, China. FAU - Yuan, Ying AU - Yuan Y AD - State Key Laboratory of Environmental Criteria and Risk Assessment, and Key Laboratory of Groundwater Pollution Simulation and Control of Ministry of Ecology and Environment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. FAU - Hui, Kunlong AU - Hui K AD - State Key Laboratory of Environmental Criteria and Risk Assessment, and Key Laboratory of Groundwater Pollution Simulation and Control of Ministry of Ecology and Environment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; State Key Laboratory of Soil Pollution Control and Safety, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: huikunlong@craes.org.cn. FAU - Tan, Wenbin AU - Tan W AD - State Key Laboratory of Environmental Criteria and Risk Assessment, and Key Laboratory of Groundwater Pollution Simulation and Control of Ministry of Ecology and Environment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Netherlands TA - J Hazard Mater JT - Journal of hazardous materials JID - 9422688 SB - IM OTO - NOTNLM OT - Bioavailability OT - Colloid-facilitated transport OT - Interfacial partitioning OT - Mixture toxicology OT - Resistance co-selection OT - Treatment rebound COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:10 PHST- 2026/06/30 00:00 [received] PHST- 2026/10/21 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:10 [entrez] AID - S0304-3894(26)03022-0 [pii] AID - 10.1016/j.jhazmat.2026.144041 [doi] PST - aheadofprint SO - J Hazard Mater. 2026 Oct 27;518:144041. doi: 10.1016/j.jhazmat.2026.144041. PMID- 42950658 OWN - NLM STAT- Publisher LR - 20261028 IS - 1872-7506 (Electronic) IS - 0925-4927 (Linking) VI - 364 DP - 2026 Oct 24 TI - MEG-based neural biomarkers and AI for early schizophrenia identification. PG - 112364 LID - S0925-4927(26)00229-5 [pii] LID - 10.1016/j.pscychresns.2026.112364 [doi] AB - Early schizophrenia detection is vital for timely intervention, and magnetoencephalography (MEG) offers a promising tool for capturing subtle neurophysiological disruptions. However, machine learning (ML) performance in psychiatric diagnosis depends heavily on feature relevance. In this study, we evaluated two distinct feature paradigms: Graph-Theoretical Metrics (GTM; 77 topological measures derived from MEG connectivity); and Expert-guided Neurocognitive and Graph-Theoretical Metrics (ENGTM; GTM integrated with 42 domain-expert-selected clinical/cognitive dimensions, such as MCCB and IQ). MEG data from 52 participants (early-stage schizophrenia vs. healthy controls) were analyzed using six ML classifiers within a nested cross-validation framework. Models trained on GTM features showed moderate performance, with AdaBoost achieving an AUC of 0.77. In contrast, ENGTM-trained models demonstrated significantly superior diagnostic capacity. The Decision Tree model reached 0.96 accuracy (0.97 sensitivity), while Random Forest and SVM achieved near-perfect precision and specificity (0.98). These findings demonstrate that expert-guided feature selection substantially enhances ML performance in MEG-based schizophrenia detection. By bridging clinical psychiatric insight with computational pipelines, this approach improves diagnostic precision and supports the development of reliable, multi-modal neuroimaging biomarkers for clinical use. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Dehesh, Tania AU - Dehesh T AD - Modeling in Health Research Center, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran; Department of Biostatistics and Epidemiology, School of Public Health, Kerman University of Medical Sciences, Kerman, Iran. FAU - Sharini, Hamid AU - Sharini H AD - Department of Medical Physics and Biomedical Engineering, School of Medicine, Kermanshah University of Medical Sciences (KUMS), Kermanshah, Iran. FAU - Solouki, Leila AU - Solouki L AD - Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran; Health Data Science Research Center, Health Research Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran. FAU - Jahani, Yones AU - Jahani Y AD - Modeling in Health Research Center, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran; Medical Informatics Research Center, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran. FAU - Nabizadeh, Amir Hossein AU - Nabizadeh AH AD - Medical Informatics Research Center, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran; INESC-ID, Lisbon, Portugal, Lisbon, Portugal. FAU - Jafari, Samira AU - Jafari S AD - Modeling in Health Research Center, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran. Electronic address: s_jafari@kmu.ac.ir. LA - eng PT - Journal Article DEP - 20261024 PL - Netherlands TA - Psychiatry Res Neuroimaging JT - Psychiatry research. Neuroimaging JID - 101723001 SB - IM OTO - NOTNLM OT - Feature selection OT - Machine learning OT - Magnetoencephalography (MEG) OT - Neurophysiological biomarker OT - Schizophrenia detection COIS- Declaration of competing interest The authors declare that they have no conflict of interest. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:10 PHST- 2026/06/19 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:10 [entrez] AID - S0925-4927(26)00229-5 [pii] AID - 10.1016/j.pscychresns.2026.112364 [doi] PST - aheadofprint SO - Psychiatry Res Neuroimaging. 2026 Oct 24;364:112364. doi: 10.1016/j.pscychresns.2026.112364. PMID- 42950653 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-7838 (Electronic) IS - 0010-0277 (Linking) VI - 279 DP - 2026 Oct 28 TI - Working- and long-term memory target templates converge to produce coactive guidance of attention during visual search. PG - 106747 LID - S0010-0277(26)00316-1 [pii] LID - 10.1016/j.cognition.2026.106747 [doi] AB - Templates that guide attention during visual search can be maintained in visual working memory (VWM) or in long-term memory (LTM). A central challenge for theories of attention is explaining how these sources of bias combine to determine selection. Here, we tested whether they converge on a common priority signal. Specifically, we contrasted a coactive model of guidance, assuming convergence, with three non-coactive alternatives, each holding that guidance from VWM and LTM operate independently. Participants searched for the presence of a target defined by either of two cued colors. One cued color changed on each trial (dynamic, engaging VWM), whereas the other remained constant (static, engaging LTM). The target could match either one of the cued colors (single-target trials) or both (redundant-target trials). Through distributional analysis, we tested for the presence of redundant-target RTs that were faster than could have been generated by the two individual guidance processes operating independently in parallel. Such "violations" of the race model inequality (RMI) are observed only within a coactive system. For single-target trials, searches were substantially more efficient for dynamic-match targets than for static-match targets. Nevertheless, we observed robust violations of the RMI on redundant-target trials. Together, these results demonstrate that target templates can be maintained in qualitatively different states yet ultimately converge to jointly influence a common attentional priority signal. CI - Copyright (c) 2026 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Bahle, Brett AU - Bahle B AD - Department of Psychology, University of Michigan, United States of America. Electronic address: bcbahle@umich.edu. FAU - Niu, Zexuan AU - Niu Z AD - Department of Psychological and Brain Sciences, The University of Iowa, United States of America. FAU - Strueder, Jeremy AU - Strueder J AD - Department of Psychological and Brain Sciences, The University of Iowa, United States of America. FAU - Hollingworth, Andrew AU - Hollingworth A AD - Department of Psychological and Brain Sciences, The University of Iowa, United States of America. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Cognition JT - Cognition JID - 0367541 SB - IM OTO - NOTNLM OT - Long-term memory OT - Visual search OT - Working memory COIS- Declaration of competing interest The authors declare no conflicts of interest. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:10 PHST- 2026/06/24 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:10 [entrez] AID - S0010-0277(26)00316-1 [pii] AID - 10.1016/j.cognition.2026.106747 [doi] PST - aheadofprint SO - Cognition. 2026 Oct 28;279:106747. doi: 10.1016/j.cognition.2026.106747. PMID- 42950646 OWN - NLM STAT- Publisher LR - 20261028 IS - 1878-1705 (Electronic) IS - 1567-5769 (Linking) VI - 191 IP - Pt 1 DP - 2026 Oct 28 TI - Rapamycin attenuates bone cancer pain and tumor progression associated with reduced NE/NETosis-related molecular signatures in a mouse model. PG - 117610 LID - S1567-5769(26)01458-X [pii] LID - 10.1016/j.intimp.2026.117610 [doi] AB - Bone cancer pain (BCP) is a refractory condition associated with tumor metastasis for which effective treatment options remain limited. Although NETosis-related inflammatory processes and neutrophil elastase (NE) have been implicated in pathological pain, their association with BCP remains insufficiently characterized. Here, we investigated NETosis-related molecular alterations in a BCP mouse model and evaluated the analgesic and antitumor effects of rapamycin, a mammalian target of rapamycin (mTOR) inhibitor with immunomodulatory properties. The BCP model was established by injecting Lewis lung carcinoma cells into the femur, resulting in progressive mechanical allodynia, heat hyperalgesia, and spontaneous pain. Spinal transcriptomic analysis revealed enrichment of genes associated with NETosis-related processes, with upregulated expression of Elane and Mpo mRNA. Exploratory molecular docking was performed to visualize a possible interaction pattern between rapamycin and NE. In vivo, rapamycin (1.5-5 mg/kg, i.p.) produced dose-related attenuation of pain-related behaviors. The 5 mg/kg dose showed the greatest behavioral improvement and was selected for subsequent analyses. Western blotting and immunofluorescence demonstrated that rapamycin treatment was associated with reduced levels of NETosis-associated markers, including NE, MPO, and citrullinated histone H3, in the spinal cord. Rapamycin treatment was also associated with reduced local tumor growth. Collectively, these findings suggest that NE/NETosis-related molecular alterations may represent a relevant feature of BCP pathophysiology. Rapamycin attenuated pain-related behaviors and was associated with reduced local tumor burden and lower levels of NE/NETosis-related molecular markers in this mouse model, suggesting that these molecular alterations may accompany the observed beneficial effects. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Li, Wen AU - Li W AD - Medical School of Chinese PLA, Beijing, People's Republic of China; Department of Pain, First Medical Center of Chinese PLA General Hospital, Beijing, People's Republic of China. FAU - Wang, Xiaochen AU - Wang X AD - Department of Critical Care Medicine, First Medical Center of Chinese PLA General Hospital, Beijing, People's Republic of China; School of Medicine, Nankai University, Tianjin, People's Republic of China. FAU - Wang, Long AU - Wang L AD - Department of Pain, First Medical Center of Chinese PLA General Hospital, Beijing, People's Republic of China. FAU - Tang, Guo AU - Tang G AD - Department of Pain, First Medical Center of Chinese PLA General Hospital, Beijing, People's Republic of China. FAU - Li, Yang AU - Li Y AD - Department of Pain, First Medical Center of Chinese PLA General Hospital, Beijing, People's Republic of China. Electronic address: liyang4522@163.com. FAU - Feng, Zeguo AU - Feng Z AD - Medical School of Chinese PLA, Beijing, People's Republic of China; Department of Pain, First Medical Center of Chinese PLA General Hospital, Beijing, People's Republic of China. Electronic address: beijing_301@sina.com. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Int Immunopharmacol JT - International immunopharmacology JID - 100965259 SB - IM OTO - NOTNLM OT - Bone cancer pain OT - Hyperalgesia OT - Neutrophil elastase OT - Neutrophil extracellular trap formation OT - Rapamycin COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:09 PHST- 2026/08/16 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:09 [entrez] AID - S1567-5769(26)01458-X [pii] AID - 10.1016/j.intimp.2026.117610 [doi] PST - aheadofprint SO - Int Immunopharmacol. 2026 Oct 28;191(Pt 1):117610. doi: 10.1016/j.intimp.2026.117610. PMID- 42950637 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-3573 (Electronic) IS - 0039-9140 (Linking) VI - 314 DP - 2026 Oct 13 TI - Improved SERS detection of tetrahydrocannabinol using 4-aminophenol derivatization and machine learning. PG - 130735 LID - S0039-9140(26)01391-3 [pii] LID - 10.1016/j.talanta.2026.130735 [doi] AB - Fast, sensitive, and selective detection of Delta(9)-tetrahydrocannabinol (THC) is essential for forensic screening and quality control of cannabis-derived products. Although surface-enhanced Raman spectroscopy (SERS) is promising for THC detection, its practical use remains limited by insufficient sensitivity and selectivity, especially in complex cannabis-infused samples. In this work, chemical derivatization was used to enhance SERS performance for THC detection. Two commonly used colorimetric reagents, i.e., Fast Blue BB (FBBB) and 4-aminophenol (4-AP), were evaluated with silver nanorod SERS substrates. Among the tested reagents, 4-AP showed better compatibility with SERS, yielding clear and enhanced Raman fingerprints at multiple bands, with 1100 cm(-1) as the most suitable THC marker. Compared with our previous non-derivatized approach, the developed method lowered the detection limit by 20-fold, reaching 0.051 mg/L. The method also enabled spectral differentiation of THC in the presence of other prominent cannabinoids, including cannabidiol (CBD), cannabinol (CBN) and tetrahydrocannabinolic acid (THCA). Its applicability was further demonstrated by detecting THC in commercial cannabis-infused beverage samples, achieving a sub-ppm detection limit without sample extraction. Multivariate PCA and supervised machine learning models were further developed to support data analysis. Using selected THC-related bands improved chemical interpretability and provided more consistent performance across the evaluated models. Classification accuracies were 92.5-98.7% for discrimination between THC-containing and THC-free samples and 84.8-88.4% for threshold-based screening of spiked beverage samples. Overall, combining simple chemical derivatization with SERS and machine learning provides a practical approach for sensitive and selective THC analysis, with potential for screening cannabis-infused products. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Thepin, Nopphamat AU - Thepin N AD - Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, 10903, Thailand. FAU - Somboonsaksri, Pacharamon AU - Somboonsaksri P AD - National Electronics and Computer Technology Center (NECTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand. FAU - Lapkrajang, Davis AU - Lapkrajang D AD - Department of Electronic Engineering and Computer Systems, Faculty of Engineering and Industrial Technology, Silpakorn University, Nakhon Pathom, 73000, Thailand. FAU - Termsaithong, Nichakarn AU - Termsaithong N AD - National Electronics and Computer Technology Center (NECTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand. FAU - Limwichean, Saksorn AU - Limwichean S AD - National Electronics and Computer Technology Center (NECTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand. FAU - Jintamethasawat, Rungroj AU - Jintamethasawat R AD - National Electronics and Computer Technology Center (NECTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand. FAU - Botta, Raju AU - Botta R AD - National Electronics and Computer Technology Center (NECTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand. Electronic address: raju.botta@nectec.or.th. FAU - Ngernsutivorakul, Thitaphat AU - Ngernsutivorakul T AD - Department of Chemistry, Faculty of Science, Kasetsart University, Bangkok, 10903, Thailand; Center of Excellence for Innovation in Chemistry (PERCH-CIC), Ministry of Higher Education, Science, Research and Innovation (MHESI), Bangkok, 10400, Thailand. Electronic address: thitaphat.n@ku.th. LA - eng PT - Journal Article DEP - 20261013 PL - Netherlands TA - Talanta JT - Talanta JID - 2984816R SB - IM OTO - NOTNLM OT - 4-Aminophenol OT - Cannabis beverage OT - Derivatization OT - Machine learning OT - Surface-enhanced Raman spectroscopy OT - Tetrahydrocannabinol COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:09 PHST- 2026/07/21 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:09 [entrez] AID - S0039-9140(26)01391-3 [pii] AID - 10.1016/j.talanta.2026.130735 [doi] PST - aheadofprint SO - Talanta. 2026 Oct 13;314:130735. doi: 10.1016/j.talanta.2026.130735. PMID- 42950635 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-3573 (Electronic) IS - 0039-9140 (Linking) VI - 314 DP - 2026 Oct 27 TI - In situ growth of covalent organic frameworks on the surface of Cu-MOF derivatives boosting self-powered electrochemical sensor. PG - 130839 LID - S0039-9140(26)01495-5 [pii] LID - 10.1016/j.talanta.2026.130839 [doi] AB - The advancement of miniaturized and intelligent sensing nodes in Internet of Things and wearable electronics necessitates self-powered systems with high sensitivity and stability. This work developed a novel visible-light-assisted self-powered electrochemical sensor (SPES) based on zinc-air battery (ZAB). Herein, a series of composite photocathode materials, CuO(x)/C@COF-PTCDA, CuO(x)/C@COF-NTCDA, and CuO(x)/C@COF-PMDA, were designed and fabricated via the in-situ pyrolysis of copper-based metal-organic framework (CuBTC) coupled with the concurrent growth of imide-linked covalent organic frameworks (COFs). These composites combine high conductivity and abundant catalytic active sites, enhancing the oxygen reduction reaction kinetics and photocatalytic oxygen reduction activity, thereby achieving efficient synergistic conversion of chemical and solar energy. The optimized SPES-ZAB based on CuO(x)/C@COF-PTCDA delivered an open-circuit voltage of 1.48 V under illumination with excellent stability. By integrating an antifouling peptide and steric-hindrance sensing strategy, the constructed SPES-ZAB achieved highly sensitive and selective detection of human chorionic gonadotropin (hCG), exhibiting a linear range of 0.5-500 ng/mL, along with good selectivity, reproducibility, and accurate detection capability in real urine samples. This study provides a novel strategy for material design and system integration towards developing efficient, stable, and antifouling self-powered sensing platforms, advancing the practical application of intelligent sensors in health monitoring and environmental analysis. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Ying, Tianfu AU - Ying T AD - Hubei Key Laboratory for Processing and Application of Catalytic Materials, College of Chemistry and Chemical Engineering, Huanggang Normal University, Huanggang, 438000, PR China; Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, PR China. FAU - Liu, Jinghua AU - Liu J AD - Hubei Key Laboratory for Processing and Application of Catalytic Materials, College of Chemistry and Chemical Engineering, Huanggang Normal University, Huanggang, 438000, PR China. FAU - Liang, Jingxuan AU - Liang J AD - Hubei Key Laboratory for Processing and Application of Catalytic Materials, College of Chemistry and Chemical Engineering, Huanggang Normal University, Huanggang, 438000, PR China. FAU - Qin, Ziyan AU - Qin Z AD - Hubei Key Laboratory for Processing and Application of Catalytic Materials, College of Chemistry and Chemical Engineering, Huanggang Normal University, Huanggang, 438000, PR China. FAU - Shao, Dong AU - Shao D AD - Hubei Key Laboratory for Processing and Application of Catalytic Materials, College of Chemistry and Chemical Engineering, Huanggang Normal University, Huanggang, 438000, PR China. FAU - Chen, Yuhui AU - Chen Y AD - Hubei Key Laboratory for Processing and Application of Catalytic Materials, College of Chemistry and Chemical Engineering, Huanggang Normal University, Huanggang, 438000, PR China; Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, PR China. FAU - Zhu, Junlun AU - Zhu J AD - Hubei Key Laboratory for Processing and Application of Catalytic Materials, College of Chemistry and Chemical Engineering, Huanggang Normal University, Huanggang, 438000, PR China; Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, PR China. Electronic address: zhujunlun@nju.edu.cn. FAU - Zhang, Xiuhua AU - Zhang X AD - Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, PR China. FAU - Wang, Shengfu AU - Wang S AD - Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, PR China. FAU - Wen, Wei AU - Wen W AD - Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules & College of Chemistry and Chemical Engineering, Hubei University, Wuhan, 430062, PR China. Electronic address: wenwei@hubu.edu.cn. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Talanta JT - Talanta JID - 2984816R SB - IM OTO - NOTNLM OT - Antifouling OT - Covalent organic framework OT - Metal-organic framework-derived OT - Self-powered electrochemical sensor COIS- Declaration of competing interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:09 PHST- 2026/07/30 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:09 [entrez] AID - S0039-9140(26)01495-5 [pii] AID - 10.1016/j.talanta.2026.130839 [doi] PST - aheadofprint SO - Talanta. 2026 Oct 27;314:130839. doi: 10.1016/j.talanta.2026.130839. PMID- 42950633 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-3573 (Electronic) IS - 0039-9140 (Linking) VI - 314 DP - 2026 Oct 27 TI - Synergistic enhancement of laser-induced breakdown spectroscopy via Ag@Cu-MOFs/CMFP composite substrate for ultrasensitive detection of trace lead in water. PG - 130829 LID - S0039-9140(26)01485-2 [pii] LID - 10.1016/j.talanta.2026.130829 [doi] AB - Sensitive detection of lead (Pb) in water via laser-induced breakdown spectroscopy (LIBS) remains challenging due to plasma emission quenching by the aqueous matrix. To address this limitation, this study proposes a surface-enhanced LIBS (SE-LIBS) strategy coupled with analyte enrichment on a solid substrate. The substrate, denoted as Ag@Cu-MOFs/CMFP, was fabricated by growing a copper-based metal-organic framework (Cu-MOF) on carboxymethylated filter paper (CMFP), followed by deposition of silver nanoparticles (Ag NPs) through chemical reduction. The Cu-MOF layer selectively captures Pb(2+) via ion exchange, delivering a high adsorption capacity of 59.7 mg g(-1). This enrichment step effectively transfers dissolved Pb into a uniform solid matrix amenable to laser interrogation. Concurrently, Ag NPs enhance laser-matter coupling through localized surface plasmon resonance (LSPR). Plasma diagnostics revealed that the incorporation of Ag NPs increased the electron temperature by 2.1-fold, while the Pb I 405.8 nm emission intensity reached 4.3 times that of pristine filter paper. Under optimized experimental conditions, this method achieved a limit of detection (LOD) of 44.3 mug L(-1) and a relative standard deviation (RSD) of 2.8%. The substrate also maintained excellent selectivity in the presence of competing cations. Recovery tests with river-water samples gave Pb recoveries of 97-104%. By synergistically combining MOF-mediated pre-concentration with AgNPs-assisted emission enhancement, this work offers a sensitive, selective, and field-compatible approach for Pb monitoring in aquatic environments. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Wang, Bo AU - Wang B AD - Department of Nuclear Science and Technology, Nanjing University of Aeronautics and Astronautics, Nanjing, 211106, China. FAU - Jia, Wenbao AU - Jia W AD - Department of Nuclear Science and Technology, Nanjing University of Aeronautics and Astronautics, Nanjing, 211106, China; Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou, 215021, China. FAU - Dong, Wenjing AU - Dong W AD - School of Materials Science and Engineering Northwestern Polytechnical University, Xi'an, 710072, China. FAU - Shan, Qing AU - Shan Q AD - Department of Nuclear Science and Technology, Nanjing University of Aeronautics and Astronautics, Nanjing, 211106, China; Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou, 215021, China. FAU - Ling, Yongsheng AU - Ling Y AD - Department of Nuclear Science and Technology, Nanjing University of Aeronautics and Astronautics, Nanjing, 211106, China; Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Suzhou, 215021, China. Electronic address: lingyongsheng@nuaa.edu.cn. FAU - Zhang, Zhichao AU - Zhang Z AD - Jiangxi Province Key Laboratory of Nuclear Physics and Technology, East China University of Technology, Nanchang, 330013, China. Electronic address: zhangzc@ecut.edu.cn. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Talanta JT - Talanta JID - 2984816R SB - IM OTO - NOTNLM OT - Laser-induced breakdown spectroscopy OT - Lead detection OT - Metal-organic frameworks OT - Silver nanoparticles OT - Solid-phase enrichment COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:09 PHST- 2026/06/02 00:00 [received] PHST- 2026/10/23 00:00 [revised] PHST- 2026/10/24 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:09 [entrez] AID - S0039-9140(26)01485-2 [pii] AID - 10.1016/j.talanta.2026.130829 [doi] PST - aheadofprint SO - Talanta. 2026 Oct 27;314:130829. doi: 10.1016/j.talanta.2026.130829. PMID- 42950632 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-3573 (Electronic) IS - 0039-9140 (Linking) VI - 314 DP - 2026 Oct 22 TI - Methylene-tuned sulfonic acid-functionalized silica stationary phases for multi-mode chromatography. PG - 130812 LID - S0039-9140(26)01468-2 [pii] LID - 10.1016/j.talanta.2026.130812 [doi] AB - Traditional single-mode stationary phases often suffer from limited separation capability for complex samples containing both hydrophobic, hydrophilic, and ionizable components, requiring multiple column switches that reduce efficiency and increase method complexity. Mixed-mode stationary phases providing two or more retention mechanisms on a single column offer a promising solution. In this study, two novel stationary phases, phenylmethanesulfonic acid-modified silica (Sil-SMSa) and benzenesulfonic acid-modified silica (Sil-SSa), were designed and prepared via thiol-ene click chemistry, offering reversed-phase chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC), and ion exchange chromatography (IEC) modes, enabling effective separation of nucleobases and nucleosides, vitamins, flavonoids, sulfonamides, polycyclic aromatic hydrocarbons (PAHs), and rare earth elements (REEs). Compared with in-house C18 column and HILIC columns, both Sil-SMSa and Sil-SSa showed better selectivity, broader polarity coverage, and lower solvent consumption. The methylene spacer for Sil-SMSa improved sulfonic acid group (-SO(3)(-)) arrangement and hydrophilic layer order, enhancing the resolution of structurally similar compounds. In contrast, the sulfonic acid groups of Sil-SSa are directly attached to the benzene ring, the surface is more rigid and the hydrophilic layer is restricted, leading to stronger electrostatic interactions in HILIC and IEC. This comparison provides evidence for how surface structure regulates multi-mode separation performance and guides the rational design of advanced materials. This work demonstrates that subtle structural variations, such as the presence or absence of a methylene spacer, can significantly modulate separation selectivity and retention behavior, highlighting the importance of the spacer in the development of high-performance multi-mode stationary phases. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Yang, Yali AU - Yang Y AD - CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, China; Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, 730070, China. FAU - Zhang, Ying AU - Zhang Y AD - Key Laboratory of Pesticide and Veterinary Drug Monitoring, State Administration for Market Regulation, Lanzhou Institute for Food and Drug Control, Lanzhou, 730000, China. FAU - Quan, Kaijun AU - Quan K AD - CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, China. FAU - Zhao, Hongwei AU - Zhao H AD - Key Laboratory of Rare Earths, Ganjiang Innovation Academy, Chinese Academy of Sciences, Ganzhou, 341000, China. FAU - Zhang, Bo AU - Zhang B AD - Department of Chemistry and the MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China. FAU - Liu, Xiuhui AU - Liu X AD - Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, College of Chemistry & Chemical Engineering, Northwest Normal University, Lanzhou, 730070, China. FAU - Miao, Qian AU - Miao Q AD - Key Laboratory of Pesticide and Veterinary Drug Monitoring, State Administration for Market Regulation, Lanzhou Institute for Food and Drug Control, Lanzhou, 730000, China. FAU - Qiu, Hongdeng AU - Qiu H AD - CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, China; Key Laboratory of Rare Earths, Ganjiang Innovation Academy, Chinese Academy of Sciences, Ganzhou, 341000, China. FAU - Chen, Jia AU - Chen J AD - CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, China. Electronic address: jiachen@licp.cas.cn. LA - eng PT - Journal Article DEP - 20261022 PL - Netherlands TA - Talanta JT - Talanta JID - 2984816R SB - IM OTO - NOTNLM OT - Methylene spacer OT - Multi-mode stationary phase OT - Sulfonic acid-functionalized silica OT - Thiol-ene click chemistry COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:09 PHST- 2026/08/30 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:09 [entrez] AID - S0039-9140(26)01468-2 [pii] AID - 10.1016/j.talanta.2026.130812 [doi] PST - aheadofprint SO - Talanta. 2026 Oct 22;314:130812. doi: 10.1016/j.talanta.2026.130812. PMID- 42950627 OWN - NLM STAT- Publisher LR - 20261028 IS - 1878-562X (Electronic) IS - 1567-5394 (Linking) VI - 174 DP - 2026 Oct 27 TI - Structural mechanisms governing protein-electrode electron transfer: accessibility, redox relays, conformational gating, and quaternary shielding. PG - 109491 LID - S1567-5394(26)00277-X [pii] LID - 10.1016/j.bioelechem.2026.109491 [doi] AB - Direct electron transfer (DET) between redox proteins and electrodes depends on the interplay between molecular architecture and interfacial physicochemical properties. Here, we developed a computationally inexpensive structural framework integrating planar electrode accessibility, internal redox relays, conformational gating, quaternary shielding, and protein-surface chemistry. Experimentally resolved protein structures were analyzed using orientation sampling and redox-center topology. Horseradish peroxidase exhibited favorable electrode accessibility (F20 = 0.392), whereas multicofactor enzymes revealed alternative routes to electronic communication. F20 correlated inversely with molecular weight (Spearman rho = -0.883) and radius of gyration (rho = -0.737), but not with size-normalized compactness (rho = -0.069). Cellobiose dehydrogenase exhibited a pronounced conformational effect, with F20 increasing from 0.0277 to 0.2110 between closed and open states. Quaternary assembly restricted electrode accessibility in selected enzymes, while internal redox relays connected buried catalytic centers to peripheral electron-transfer sites. External evaluation using copper nitrite reductase and [NiFe]‑hydrogenase supported the structural interpretation of relay organization and electrode accessibility. Importantly, comparable geometric accessibility did not necessarily imply equivalent electrochemical competence, emphasizing the influence of surface chemistry, redox energetics, and electrode-interface interactions. The framework provides a reproducible, mechanism-oriented approach for interpreting protein-electrode communication and identifying experimentally testable strategies for bioelectrocatalytic interface design. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Castillo, John J AU - Castillo JJ AD - Grupo de Investigacion en Bioquimica y Microbiologia, Universidad Industrial de Santander, Bucaramanga, Santander, Colombia. Electronic address: jcasleon@uis.edu.co. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Bioelectrochemistry JT - Bioelectrochemistry (Amsterdam, Netherlands) JID - 100953583 SB - IM OTO - NOTNLM OT - Bioelectrocatalysis OT - Conformational gating OT - Direct electron transfer OT - Electron-transfer relays OT - Protein electrochemistry OT - Protein-electrode interface OT - Redox enzymes COIS- Declaration of competing interest The author declares that he/she have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:09 PHST- 2026/09/28 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:09 [entrez] AID - S1567-5394(26)00277-X [pii] AID - 10.1016/j.bioelechem.2026.109491 [doi] PST - aheadofprint SO - Bioelectrochemistry. 2026 Oct 27;174:109491. doi: 10.1016/j.bioelechem.2026.109491. PMID- 42950626 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-4235 (Electronic) IS - 0956-5663 (Linking) VI - 317 DP - 2026 Oct 24 TI - A one-step functionalized 3D-printed electrochemical biosensor for serotype-selective detection of dengue NS1. PG - 119362 LID - S0956-5663(26)00995-4 [pii] LID - 10.1016/j.bios.2026.119362 [doi] AB - Rapid and selective detection of dengue virus serotypes remains challenging because of the co-circulation of antigenically related arboviruses. We developed a fully 3D-printed electrochemical biosensor for dengue virus serotype 2 non-structural protein 1 (DENV-2 NS1). The integrated three-electrode platform incorporated a graphite/polylactic acid working electrode modified in a single drop-casting step with preassembled monoclonal antibody-gold nanoparticle conjugates (mAb-AuNP). DENV-2 NS1 binding was monitored by cyclic voltammetry using [Fe(CN)(6)](3-/4-) as a redox probe; immunocomplex formation partially blocked interfacial electron transfer and decreased the cathodic peak current. The response stabilized after 15 min and was linear from 0.15 to 0.75 mug L(-1) (1.75-8.74 pM), with R(2) = 0.9922 and a limit of detection of 0.061 mug L(-1) (0.71 pM). Three independently prepared biosensors showed a relative standard deviation of 3.4%, indicating good fabrication reproducibility. The immunosensor produced a substantially greater response to DENV-2 NS1 than to DENV-1 NS1, Zika virus NS1, or chikungunya virus E2 protein. In 100-fold-diluted human serum spiked at 0.15 and 0.25 mug L(-1), standard-addition analysis yielded recoveries of 106.0 +/- 1.3% and 97.7 +/- 1.1%, respectively. These findings demonstrate that one-step mAb-AuNP functionalization of fully 3D-printed electrodes provides a rapid, reproducible, and serotype-selective approach to NS1 detection, warranting further validation with clinical samples. CI - Copyright (c) 2026 The Authors. Published by Elsevier B.V. All rights reserved. FAU - D'Amato, Dayenny L AU - D'Amato DL AD - Departamento de Quimica Inorganica, Universidade Federal Fluminense, Campus do Valonguinho, Outeiro Sao Joao Batista s/n, Centro, Niteroi, RJ, 24020-150, Brazil. FAU - Caldas, Natalia M AU - Caldas NM AD - Departamento de Quimica Analitica, Universidade Federal Fluminense, Campus do Valonguinho, Outeiro Sao Joao Batista s/n, Centro, Niteroi, RJ, 24020-150, Brazil. FAU - de Faria, Lucas V AU - de Faria LV AD - Departamento de Quimica Analitica, Universidade Federal do Rio de Janeiro, Campus Fundao, Avenida Athos da Silveira Ramos 149, Cidade Universitaria, Rio de Janeiro, RJ, 21941-590, Brazil. FAU - Ligiero, Carolina B P AU - Ligiero CBP AD - Departamento de Quimica Inorganica, Universidade Federal Fluminense, Campus do Valonguinho, Outeiro Sao Joao Batista s/n, Centro, Niteroi, RJ, 24020-150, Brazil. FAU - Souza, Ana Beatriz C AU - Souza ABC AD - Departamento de Quimica Inorganica, Universidade Federal Fluminense, Campus do Valonguinho, Outeiro Sao Joao Batista s/n, Centro, Niteroi, RJ, 24020-150, Brazil. FAU - de Mello, Camille C AU - de Mello CC AD - Departamento de Quimica Inorganica, Universidade Federal Fluminense, Campus do Valonguinho, Outeiro Sao Joao Batista s/n, Centro, Niteroi, RJ, 24020-150, Brazil. FAU - Oliveira, Guilherme P AU - Oliveira GP AD - Departamento de Quimica Analitica, Universidade Federal Fluminense, Campus do Valonguinho, Outeiro Sao Joao Batista s/n, Centro, Niteroi, RJ, 24020-150, Brazil. FAU - Dornellas, Rafael M AU - Dornellas RM AD - Departamento de Quimica Analitica, Universidade Federal Fluminense, Campus do Valonguinho, Outeiro Sao Joao Batista s/n, Centro, Niteroi, RJ, 24020-150, Brazil. Electronic address: rafaeldornellas@id.uff.br. FAU - Ronconi, Celia M AU - Ronconi CM AD - Departamento de Quimica Inorganica, Universidade Federal Fluminense, Campus do Valonguinho, Outeiro Sao Joao Batista s/n, Centro, Niteroi, RJ, 24020-150, Brazil. Electronic address: cmronconi@id.uff.br. LA - eng PT - Journal Article DEP - 20261024 PL - England TA - Biosens Bioelectron JT - Biosensors & bioelectronics JID - 9001289 SB - IM OTO - NOTNLM OT - 3D-printed electrode OT - Antibody-gold nanoparticle conjugates OT - Dengue virus serotype 2 (DENV-2) OT - Electrochemical biosensor OT - NS1 protein OT - Serotype-selective detection COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:09 PHST- 2026/06/15 00:00 [received] PHST- 2026/08/21 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:09 [entrez] AID - S0956-5663(26)00995-4 [pii] AID - 10.1016/j.bios.2026.119362 [doi] PST - aheadofprint SO - Biosens Bioelectron. 2026 Oct 24;317:119362. doi: 10.1016/j.bios.2026.119362. PMID- 42950625 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-4235 (Electronic) IS - 0956-5663 (Linking) VI - 317 DP - 2026 Oct 26 TI - Plant-adaptive fluorescence hydrogel sensor for non-invasive monitoring of pesticide degradation. PG - 119365 LID - S0956-5663(26)00998-X [pii] LID - 10.1016/j.bios.2026.119365 [doi] AB - Addressing the global food shortage crisis requires precise and timely diagnostics of plant exogenous stresses (such as pesticides) to enhance the quality and yield of crops. Plant-wearable sensors (PWS) offer a promising approach to real-time monitoring of pesticides for plant health but remain limited by challenges in low signal-conversion efficiency and poor mechanical adaptability at the plant-sensor interface. Here we report a robust PWS by embedding an aggregation-induced emission (AIE) silver nanoclusters@zeolitic imidazolate framework@polydopamine (AgNCs@ZIF@PDA) composite into a plant-adaptive hydrogel patch, enabling the on-site monitoring of pesticide information. The conformal hydrogel interface of the PWS utilizes an in-situ radical triggered sol-gel phase transition to lock into the abrupt hairy surface irregularities, resolving the issue of mechanical adaptability. The resultant AgNCs@ZIF@PDA-based hydrogel patch exhibits high sensitivity toward 2,4-dichlorophenoxyacetic acid, with a limit of detection as low as 26.7 ng/mL. Mechanism investigations elucidate that the synergistic combination of the AIE effect of AgNCs and the specific responsive behavior of the heterogeneous interface of PDA enhances the energy-transfer (signal-conversion) efficiency. Notably, the PWS enables non-invasive, continuous quantification of pesticide degradation kinetics. This robust fluorescence PWS enables real-time tracking of pesticides for plant health, thereby advancing the goals of precision chemical application and resource-efficient agriculture. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Huang, Zihao AU - Huang Z AD - Department of Food Quality and Safety College of Food Science and Engineering, Jilin University, Changchun, 130062, PR China. FAU - Wen, Liujun AU - Wen L AD - Department of Food Quality and Safety College of Food Science and Engineering, Jilin University, Changchun, 130062, PR China. FAU - Yan, Xu AU - Yan X AD - Key Laboratory of Advanced Gas Sensors, College of Electronic Science and Engineering, Jilin University, Changchun, 130012, PR China. FAU - Lin, Zhen AU - Lin Z AD - State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, 130012, PR China. FAU - Yao, Dong AU - Yao D AD - State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, 130012, PR China. FAU - Zhang, Lening AU - Zhang L AD - Department of Thoracic Surgery, China-Japan Union Hospital, Jilin University, Changchun, 130033, PR China. Electronic address: zhanglening@jlu.edu.cn. FAU - Li, Hongxia AU - Li H AD - Department of Food Quality and Safety College of Food Science and Engineering, Jilin University, Changchun, 130062, PR China; Key Laboratory of Advanced Gas Sensors, College of Electronic Science and Engineering, Jilin University, Changchun, 130012, PR China. Electronic address: hxiali@jlu.edu.cn. LA - eng PT - Journal Article DEP - 20261026 PL - England TA - Biosens Bioelectron JT - Biosensors & bioelectronics JID - 9001289 SB - IM OTO - NOTNLM OT - Hydrogel patch OT - In-situ OT - Pesticide OT - Plant health OT - Plant-wearable sensor COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:09 PHST- 2026/06/30 00:00 [received] PHST- 2026/10/21 00:00 [revised] PHST- 2026/10/24 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:09 [entrez] AID - S0956-5663(26)00998-X [pii] AID - 10.1016/j.bios.2026.119365 [doi] PST - aheadofprint SO - Biosens Bioelectron. 2026 Oct 26;317:119365. doi: 10.1016/j.bios.2026.119365. PMID- 42950613 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-2782 (Electronic) IS - 0893-6080 (Linking) VI - 206 IP - Pt B DP - 2026 Oct 23 TI - Memory dendritic neural P systems for multi-ground type fault line selection in distribution networks. PG - 109795 LID - S0893-6080(26)01250-5 [pii] LID - 10.1016/j.neunet.2026.109795 [doi] AB - Rapid and accurate single-line-to-ground (SLG) fault line selection in distribution networks is crucial to power system safety. Although existing deep learning methods have achieved good performance, there is still room for improvement in the accuracy of fault line selection when faced with multiple neutral grounding types and varying fault conditions. The neural-like P systems use the "point neurons" computing mode and fails to fully utilize the multi-dendritic branching characteristics of biological neurons, resulting in limited computing efficiency. Therefore, this paper proposes a novel memory dendritic neural P (MDNP) system, which effectively improves the system's fault feature learning ability under different grounding types by introducing the triggering rules of memory dendritic factors, dynamically adjustable dendritic structure and adaptive learning mechanism, significantly reduces computing resource consumption and accelerates convergence speed. Furthermore, the design of online application scheme enables the MDNP system to achieve online optimization of fault line selection in dynamic power grid. The experimental results show that compared to seven baseline models, the MDNP system achieves fault line selection accuracy of 98.2% and 97.3% under non-grounding and arc suppression coil grounding types, respectively. The average fault line selection time is only 1.78s. Additionally, the computational resource consumption is reduced by 56.6% compared to traditional neural-like P systems, demonstrating its exceptional performance and potential for fault line selection tasks. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Ma, Song AU - Ma S AD - National and Local Joint Engineering Laboratory for Renewable Energy Access to Grid Technology, Hefei University of Technology, Hefei, Anhui 230009, China. Electronic address: masong0007@163.com. FAU - Li, Fei AU - Li F AD - National and Local Joint Engineering Laboratory for Renewable Energy Access to Grid Technology, Hefei University of Technology, Hefei, Anhui 230009, China. Electronic address: lifei@hfut.edu.cn. FAU - Yin, Xiu AU - Yin X AD - Business School, Shandong Province Brain-lnspired lntelligence Future Laboratory, Shandong Normal University, Jinan, Shandong 250014, China. Electronic address: xiuyinsdnu@163.com. FAU - Liu, Xiyu AU - Liu X AD - Business School, Shandong Province Brain-lnspired lntelligence Future Laboratory, Shandong Normal University, Jinan, Shandong 250014, China. Electronic address: xyliu@sdnu.edu.cn. FAU - Hu, Yangheng AU - Hu Y AD - School of Automation, Chengdu University of Information Technology, No. 24 Block 1, Xuefu Road, Chengdu, Sichuan 610225, China. Electronic address: huyhctu@163.com. FAU - Zhang, Xing AU - Zhang X AD - National and Local Joint Engineering Laboratory for Renewable Energy Access to Grid Technology, Hefei University of Technology, Hefei, Anhui 230009, China. Electronic address: honglf@ustc.edu.cn. FAU - Ma, Mingyao AU - Ma M AD - National and Local Joint Engineering Laboratory for Renewable Energy Access to Grid Technology, Hefei University of Technology, Hefei, Anhui 230009, China. Electronic address: miyama@hfut.edu.cn. LA - eng PT - Journal Article DEP - 20261023 PL - United States TA - Neural Netw JT - Neural networks : the official journal of the International Neural Network Society JID - 8805018 SB - IM OTO - NOTNLM OT - Distribution network OT - Fault line selection OT - Memory dendritic OT - Neural-like P systems OT - Single-line-to-ground COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:08 PHST- 2025/07/20 00:00 [received] PHST- 2026/07/11 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:08 [entrez] AID - S0893-6080(26)01250-5 [pii] AID - 10.1016/j.neunet.2026.109795 [doi] PST - aheadofprint SO - Neural Netw. 2026 Oct 23;206(Pt B):109795. doi: 10.1016/j.neunet.2026.109795. PMID- 42950609 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-4076 (Electronic) IS - 1879-4068 (Linking) VI - 18 IP - 2 DP - 2026 Oct 28 TI - Conversational artificial intelligence for geriatric screening: A mixed-methods feasibility study. PG - 104662 LID - S1879-4068(26)01819-9 [pii] LID - 10.1016/j.jgo.2026.104662 [doi] AB - INTRODUCTION: Geriatric assessment (GA) can identify clinically relevant vulnerabilities in older adults, but remains underused because of time, staffing, and workflow barriers. Conversational artificial intelligence (AI) may provide a scalable approach to support geriatric screening within clinical practice. MATERIALS AND METHODS: We conducted a two-phase mixed-methods feasibility study evaluating Geriatric Risk Assessment and Capability Evaluation (GRACE), a conversational AI platform for selected structured Practical Geriatric Assessment (PGA)-based screening items, compared with manual PGA assessment. Seventy adults aged >/=65 years were recruited from an academic health system and affiliated community sites. Phase 1 (n = 46) informed platform development and refinement, and Phase 2 (n = 24) evaluated the refined platform. Screening performance was evaluated at the scored-item level using sensitivity, specificity, predictive values, Cohen's kappa, and Gwet's AC1. Usability was measured with the System Usability Scale (SUS) and semi-structured interviews with participants and clinicians explored acceptability and implementation. RESULTS: In Phase 1 (n = 46), GRACE demonstrated sensitivity of 0.68, specificity of 0.96, and negative predictive value (NPV) of 0.96. Following platform refinement, Phase 2 (n = 24) demonstrated sensitivity of 0.77, specificity of 0.97, positive predictive value of 0.72, NPV of 0.98, Cohen's kappa of 0.72, and Gwet's AC1 of 0.94. Phase 2 represented the primary estimate of refined-platform performance; overall Phase 1-to-Phase 2 differences were numerical rather than statistically significant. Descriptively pooled across all 70 participants, sensitivity was 0.71, specificity 0.96, NPV 0.97, kappa 0.65, and AC1 0.92. Performance varied across domains, with stronger performance in several structured domains and lower sensitivity in functional and psychosocial domains. Participants generally reported favorable usability, and patient and clinician interviews supported a potential role for GRACE in pre-visit information gathering and targeted clinician follow-up. DISCUSSION: The refined GRACE platform demonstrated promising performance for structured geriatric screening, including high specificity and NPV, supporting its potential role in pre-visit screening and prioritization of clinician follow-up. GRACE should be used as a clinician-guided, human-in-the-loop screening tool rather than as a standalone method for excluding geriatric deficits because GRACE showed lower sensitivity in some domains and NPV depends on prevalence. Larger prospective studies are needed to validate the refined platform and evaluate its implementation and clinical impact. CI - Copyright (c) 2026 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Naeim, Arash AU - Naeim A AD - UCLA Division of Hematology-Oncology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA. Electronic address: anaeim@mednet.ucla.edu. FAU - Cheng, Justin AU - Cheng J AD - UCLA Division of Hematology-Oncology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA. FAU - Spiegel, Brennan AU - Spiegel B AD - Cedars-Sinai Center for Virtual Medicine and Health System Transformation, Los Angeles, CA, USA. FAU - Bakshian, Alexandra AU - Bakshian A AD - Center for AI & SMART Health, UCLA Clinical and Translational Science Institute Los Angeles, CA, USA. FAU - Tetrault, Ella AU - Tetrault E AD - Cedars-Sinai Center for Virtual Medicine and Health System Transformation, Los Angeles, CA, USA. FAU - Mehra, Muskaan AU - Mehra M AD - Cedars-Sinai Center for Virtual Medicine and Health System Transformation, Los Angeles, CA, USA. FAU - Amini, Dian AU - Amini D AD - Center for AI & SMART Health, UCLA Clinical and Translational Science Institute Los Angeles, CA, USA. FAU - Krut, Zoe AU - Krut Z AD - Cedars-Sinai Center for Virtual Medicine and Health System Transformation, Los Angeles, CA, USA. FAU - Shang, Helen AU - Shang H AD - UCLA Division of Hematology-Oncology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA. FAU - Sedrak, Mina S AU - Sedrak MS AD - UCLA Division of Hematology-Oncology, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA. FAU - Bui, Alex AU - Bui A AD - Center for AI & SMART Health, UCLA Clinical and Translational Science Institute Los Angeles, CA, USA. FAU - Johnson, Taylor AU - Johnson T AD - Center for AI & SMART Health, UCLA Clinical and Translational Science Institute Los Angeles, CA, USA. FAU - Wang, Raymond AU - Wang R AD - Center for AI & SMART Health, UCLA Clinical and Translational Science Institute Los Angeles, CA, USA. FAU - Petruse, Antonia AU - Petruse A AD - Center for AI & SMART Health, UCLA Clinical and Translational Science Institute Los Angeles, CA, USA. FAU - Yiqun, Jiang AU - Yiqun J AD - UCLA Department of Medicine Statistical Analysis Core, Los Angeles, CA, USA. FAU - Liran, Omer AU - Liran O AD - Cedars-Sinai Center for Virtual Medicine and Health System Transformation, Los Angeles, CA, USA. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - J Geriatr Oncol JT - Journal of geriatric oncology JID - 101534770 SB - IM OTO - NOTNLM OT - Conversational artificial intelligence OT - Geriatric assessment OT - Large language models OT - Older adults OT - Screening COIS- Declaration of competing interest Omer Liran is a co-founder of and holds an equity interest in VRx. Arash Naeim was a co-founder of and held an equity interest in Invista Health, which is no longer operating. All other authors declare no competing interests. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:08 PHST- 2026/01/29 00:00 [received] PHST- 2026/09/09 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:08 [entrez] AID - S1879-4068(26)01819-9 [pii] AID - 10.1016/j.jgo.2026.104662 [doi] PST - aheadofprint SO - J Geriatr Oncol. 2026 Oct 28;18(2):104662. doi: 10.1016/j.jgo.2026.104662. PMID- 42950598 OWN - NLM STAT- Publisher LR - 20261028 IS - 2950-4333 (Electronic) IS - 2950-4333 (Linking) VI - 74 IP - 6 DP - 2026 Oct 28 TI - Cross-cultural adaptation, consensus using Delphi method and content validity of the first French emergency department patient-reported experience measure. PG - 203801 LID - S2950-4333(26)00635-X [pii] LID - 10.1016/j.jeph.2026.203801 [doi] AB - AIM: The aims of this study were 1) to perform cross-cultural adaptation of the ED PREM into the French ED PREM; 2) to seek consensus on this version using the Delphi method and 3) establish its content validity. METHODS: This study was designed following the Consensus-based Standards for the selection of health Measurement Instruments guidelines. A cross-cultural adaptation of the ED PREM, renamed the French ED PREM, was performed. Consensus was sought through a Delphi method involving an expert committee, including the original developer and patient partners. Content validity was assessed with 50 patients and 7 healthcare professionals in the first round, and 10 patients in the second round, evaluating the relevance, comprehensibility, and comprehensiveness of the French ED PREM. The endpoint was an item-level content validity index (I-CVI) > 0.78 and a scale-level content validity index (S-CVI) > 0.90 without suggestions for revision. RESULTS: Consensus was achieved in two rounds, with 100% expert participation and agreement. All French ED PREM items achieved a robust content validity (I-CVI > 0.78, S-CVI > 0,90) and demonstrated excellent agreement among evaluators beyond chance (k > 0.74). CONCLUSION: Through a rigorous cross-cultural adaptation of the ED PREM, this international collaborative research has demonstrated the content validity of the French ED PREM. This work contributes to the international harmonization of patient experience measures and may facilitate future comparisons across healthcare systems. CI - Copyright (c) 2026 Elsevier Masson SAS. All rights reserved. FAU - Tiberti, Nadia AU - Tiberti N AD - Emergency Department, Aix-Pertuis Hospital, avenue des Tamaris, F-13616, Aix-en-Provence cedex 1, France; Faculty of Medical and Paramedical Sciences, Aix-Marseille University, Center for Studies and Research on Health Services and Quality of Life (CEReSS, EA 3279), 27 Boulevard Jean Moulin, F-13385 Marseille Cedex 07, France. Electronic address: nadia.tiberti@univ-amu.fr. FAU - Bull, Claudia AU - Bull C AD - Queensland Centre for Mental Health Research, The Faculty of Health, Medicine and Behavioural Sciences, The University of Queensland, QLD, Australia. FAU - Chenou, Aline AU - Chenou A AD - Emergency Department, Strasbourg University Hospital, F-67200, Strasbourg, France; ICube Laboratory UMR 7357 team IMAGeS, University of Strasbourg, France. FAU - Boyer, Laurent AU - Boyer L AD - Faculty of Medical and Paramedical Sciences, Aix-Marseille University, Center for Studies and Research on Health Services and Quality of Life (CEReSS, EA 3279), 27 Boulevard Jean Moulin, F-13385 Marseille Cedex 07, France. FAU - Douillet, Delphine AU - Douillet D AD - Emergency Department, Health Faculty, Angers University Hospital, Angers, France; UMR MitoVasc CNRS 6015 - INSERM 1083, Equipe CARME, UNIV Angers, equipe de recherche CARE, Angers, France. FAU - Colson, Sebastien AU - Colson S AD - Faculty of Medical and Paramedical Sciences, Aix-Marseille University, Center for Studies and Research on Health Services and Quality of Life (CEReSS, EA 3279), 27 Boulevard Jean Moulin, F-13385 Marseille Cedex 07, France. LA - eng PT - Journal Article DEP - 20261028 PL - France TA - J Epidemiol Popul Health JT - Journal of epidemiology and population health JID - 9918769574106676 SB - IM OTO - NOTNLM OT - Emergency medical services OT - Patient centred care OT - Patient reported experience measures OT - Quality indicators COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:08 PHST- 2026/04/03 00:00 [received] PHST- 2026/07/01 00:00 [revised] PHST- 2026/08/16 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:08 [entrez] AID - S2950-4333(26)00635-X [pii] AID - 10.1016/j.jeph.2026.203801 [doi] PST - aheadofprint SO - J Epidemiol Popul Health. 2026 Oct 28;74(6):203801. doi: 10.1016/j.jeph.2026.203801. PMID- 42950590 OWN - NLM STAT- Publisher LR - 20261028 IS - 1095-8673 (Electronic) IS - 0022-4804 (Linking) VI - 328 DP - 2026 Oct 28 TI - Barriers and Priorities for Strengthening Prehospital Trauma Care in Hawassa, Ethiopia. PG - 229-237 LID - S0022-4804(26)00625-6 [pii] LID - 10.1016/j.jss.2026.09.034 [doi] AB - INTRODUCTION: Prehospital emergency care remains underdeveloped in Ethiopia, particularly outside Addis Ababa. This study assessed frontline provider perspectives on barriers and priorities for strengthening prehospital trauma care in Hawassa. METHODS: We surveyed 57 emergency care personnel at Hawassa University Comprehensive Specialized Hospital and Hawassa Dispatch Center in April-May 2025. The 35-question survey, adapted from the World Health Organization Prehospital Emergency Care Assessment Tool, assessed infrastructure, workforce, protocols, communication, and governance. Direct operational questions were analyzed among prehospital personnel only. Questions addressing the prehospital-hospital interface were summarized separately for prehospital and hospital-based personnel. RESULTS: Among prehospital personnel, long distances to facilities were the leading infrastructure challenge (47%, 7/15), whereas inadequate training (47%, 7/15) and burnout (40%, 6/15) were the main workforce concerns. Airway management was the top training priority (67%, 10/15 first choice). Top protocol gaps were poor adherence (40%, 6/15) and inadequate training (27%, 4/15). Among prehospital and hospital-based personnel, respectively, equipment use and maintenance was selected as the leading training need by 40% (6/15) and 33% (14/42), whereas emergency medical procedures were selected by 33% (5/15) and 29% (12/42). Staff recruitment and retention was the leading funding priority in both groups, selected by 47% (7/15) and 45% (19/42), respectively. Improved infrastructure and road conditions were selected as the highest priority for addressing ambulance response times among 67% (10/15) and 62% (26/42), respectively. CONCLUSIONS: Emergency care personnel identified gaps in infrastructure, equipment, training, protocols, and coordination. Priorities for intervention included restoring the emergency hotline, standardizing ambulance equipment, providing xABCDE-focused training, and implementing standardized handover protocols. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Abu-Hamdan, Fayrouz AU - Abu-Hamdan F AD - Division of Trauma and Acute Care Surgery, Department of Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin. Electronic address: fabuhamdan@mcw.edu. FAU - Abuaqel, Dalal AU - Abuaqel D AD - Division of Trauma and Acute Care Surgery, Department of Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin. FAU - Tesfasilassie, Tomas AU - Tesfasilassie T AD - Department of Surgery, Columbia University Irving Medical Center, New York, New York. FAU - Gari, Taye AU - Gari T AD - Hawassa University Comprehensive Specialized Hospital, Hawassa University College of Medicine and Health Sciences, Hawassa, Ethiopia. FAU - Mellese, Belay AU - Mellese B AD - Hawassa University Comprehensive Specialized Hospital, Hawassa University College of Medicine and Health Sciences, Hawassa, Ethiopia. FAU - Taafesse, Haben AU - Taafesse H AD - Hawassa University Comprehensive Specialized Hospital, Hawassa University College of Medicine and Health Sciences, Hawassa, Ethiopia. FAU - Wirtu, Zemedkun Motera AU - Wirtu ZM AD - Hawassa University Comprehensive Specialized Hospital, Hawassa University College of Medicine and Health Sciences, Hawassa, Ethiopia. FAU - Shimber, Emnet Tesfaye AU - Shimber ET AD - Hawassa University Comprehensive Specialized Hospital, Hawassa University College of Medicine and Health Sciences, Hawassa, Ethiopia. FAU - Jayaraman, Sudha AU - Jayaraman S AD - Departments of Surgery and Biomedical Engineering, University of Utah School of Medicine, Salt Lake City, Utah. FAU - Zafar, Syed Nabeel AU - Zafar SN AD - Department of Surgery, University of Wisconsin-Madison School of Medicine and Public Health, Madison, Wisconsin. FAU - Dodgion, Christopher AU - Dodgion C AD - Division of Trauma and Acute Care Surgery, Department of Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin. FAU - Iverson, Katherine R AU - Iverson KR AD - Division of Trauma and Acute Care Surgery, Department of Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Surg Res JT - The Journal of surgical research JID - 0376340 SB - IM OTO - NOTNLM OT - Emergency medical services OT - Ethiopia OT - Low- and middle-income countries OT - Prehospital emergency care OT - Trauma systems OT - Workforce capacity EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:08 PHST- 2026/06/11 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:08 [entrez] AID - S0022-4804(26)00625-6 [pii] AID - 10.1016/j.jss.2026.09.034 [doi] PST - aheadofprint SO - J Surg Res. 2026 Oct 28;328:229-237. doi: 10.1016/j.jss.2026.09.034. PMID- 42950582 OWN - NLM STAT- Publisher LR - 20261028 IS - 1525-1438 (Electronic) IS - 1048-891X (Linking) VI - 36 IP - 12 DP - 2026 Sep 23 TI - International expert consensus on the contemporary role of pelvic exenteration in recurrent or persistent cervical cancer. PG - 105629 LID - S1048-891X(26)01160-6 [pii] LID - 10.1016/j.ijgc.2026.105629 [doi] AB - OBJECTIVE: To evaluate how the role of pelvic exenteration in locally recurrent or persistent cervical cancer has evolved in the era of modern systemic therapies and radiotherapy techniques and to assess the current expert perspectives on patient selection, treatment sequencing, and surgical management. METHODS: An international 2-step modified Delphi survey was conducted following a dedicated expert meeting on pelvic exenteration in cervical cancer held at the European Institute of Oncology, Milan, Italy, in October 2025. The faculty members of the scientific meeting were invited to participate in the survey after the presentation and discussion of the available evidence. The questionnaire included 33 statements across 3 domains: (1) patient selection criteria for pelvic exenteration, (2) alternatives to pelvic exenteration, including novel systemic therapies and advanced re-irradiation strategies, and (3) surgical management and reconstructive techniques. Items were rated using a 5-point Likert scale. Consensus was predefined as >/=70% agreement (scores 4-5) or disagreement (scores 1-2). RESULTS: Fifteen experts from 15 different institutions across 5 countries completed the survey. Consensus was achieved for 23 (69.7%) statements. Experts agreed that non-human papillomavirus-associated histologies and high-grade tumors should not be considered contraindications to pelvic exenteration. No specific age or body mass index cut-offs were identified as independently contraindicating surgery, whereas performance status and psychosocial support were deemed essential factors in patient selection. Complete agreement was reached regarding peritoneal dissemination and distant metastatic disease, including para-aortic lymph nodes, as contraindications to pelvic exenteration. The presence of a single positive pelvic lymph node was not generally considered a contraindication to surgery; however, 67% of respondents agreed that the presence of multiple positive pelvic lymph nodes should preclude pelvic exenteration. No consensus was reached regarding non-central disease location, tumor size cut-offs, or the impact of disease timing (persistence versus recurrence). Novel systemic therapies, including immunotherapy and antibody-drug conjugates, were generally preferred over pelvic exenteration unless severe local symptoms, such as fistulas, pelvic pain, and tumor necrosis, required immediate local control. By contrast, re-irradiation strategies generated less agreement, with pelvic exenteration generally being favored over re-irradiation only when the latter was technically not feasible or unlikely to provide durable local control. Most experts considered neoadjuvant therapy appropriate in selected patients with a high risk of positive margins or poor performance status. Minimally invasive surgery was not recognized as a standard approach. Regarding reconstructive techniques, consensus favored the formation of an ileal conduit and terminal colostomy for urinary and gastrointestinal diversion, respectively, and vertical rectus abdominis myocutaneous or combined flaps to avoid empty pelvis syndrome and for perineal or pelvic floor reconstruction. CONCLUSIONS: The role of pelvic exenteration in recurrent or persistent cervical cancer has evolved within an increasingly multidisciplinary and personalized treatment landscape. Contemporary expert opinion supports prioritizing modern systemic therapies and re-irradiation strategies over pelvic exenteration initially. Nevertheless, pelvic exenteration continues to represent a potentially curative option in carefully selected patients when complete resection is achievable and non-surgical alternatives have failed, are not feasible, or are unlikely to provide durable local control. CI - Copyright (c) 2026 European Society of Gynaecological Oncology and the International Gynecologic Cancer Society. Published by Elsevier Inc. All rights reserved. FAU - Caruso, Giuseppe AU - Caruso G AD - European Institute of Oncology (IEO) IRCCS, Gynecologic Oncology Program, Milan, Italy; University of Milan, Department of Oncology and Hemato-Oncology, Milan, Italy; Early Career IJGC Editorial Board Member, International Journal of Gynecological Cancer. Electronic address: giuseppe.caruso@ieo.it. FAU - Casaccia Giordano, Filippo AU - Casaccia Giordano F AD - European Institute of Oncology (IEO) IRCCS, Gynecologic Oncology Program, Milan, Italy; University of Milan, Department of Oncology and Hemato-Oncology, Milan, Italy. FAU - Zanagnolo, Vanna AU - Zanagnolo V AD - European Institute of Oncology (IEO) IRCCS, Gynecologic Oncology Program, Milan, Italy. FAU - Aloisi, Alessia AU - Aloisi A AD - European Institute of Oncology (IEO) IRCCS, Gynecologic Oncology Program, Milan, Italy. FAU - Abu-Rustum, Nadeem R AU - Abu-Rustum NR AD - Memorial Sloan Kettering Cancer Center, Gynecology Service, Department of Surgery, New York, NY, USA; Weill Cornell Medical College, Department of Obstetrics and Gynecology, New York, NY, USA. FAU - Angioli, Roberto AU - Angioli R AD - Campus Bio-Medico University Hospital of Rome, Department of Obstetrics and Gynecology, Rome, Italy. FAU - Chiantera, Vito AU - Chiantera V AD - Fondazione Pascale, Istituto Nazionale dei Tumori, Napoli, Italy. FAU - Chiva, Luis AU - Chiva L AD - Clinica Universidad de Navarra, Department of Gynecology and Obstetrics, Madrid, Spain. FAU - Cibula, David AU - Cibula D AD - General University Hospital, First Medical Faculty of the Charles University, Department of Gynaecology, Obstetrics and Neonatology, Prague, Czech Republic; Editorial Board Member, International Journal of Gynecological Cancer. FAU - Fagotti, Anna AU - Fagotti A AD - Fondazione Policlinico A. Gemelli IRCCS and Catholic University of the Sacred Heart, Gynecologic Oncology Unit, Rome, Italy. FAU - Fotopoulou, Christina AU - Fotopoulou C AD - Editorial Board Member, International Journal of Gynecological Cancer; Imperial College London, Department of Surgery and Cancer, Faculty of Medicine, London, United Kingdom. FAU - Gadducci, Angiolo AU - Gadducci A AD - University of Pisa, Department of Clinical and Experimental Medicine, Division of Gynecology and Obstetrics, Pisa, Italy. FAU - Kumar, Amanika AU - Kumar A AD - Mayo Clinic, Department of Obstetrics and Gynecology, Rochester, MN, USA. FAU - Landoni, Fabio AU - Landoni F AD - Humanitas San Pio X, Gynecologic Oncology Surgical Division, Milan, Italy. FAU - Raspagliesi, Francesco AU - Raspagliesi F AD - Fondazione IRCCS Istituto Nazionale dei Tumori di Milano, Gynecologic Oncology Unit, Milan, Italy. FAU - Scollo, Paolo AU - Scollo P AD - Azienda Ospedaliera per l'Emergenza Cannizzaro, Catania, Italy; Kore University of Enna, Department of Medicine and Surgery, Enna, Italy. FAU - Tozzi, Roberto AU - Tozzi R AD - University of Padua, Department of Women and Children's Health, Unit of Gynecology and Obstetrics, Padua, Italy. FAU - Vizzielli, Giuseppe AU - Vizzielli G AD - University of Udine, Department of Medicine, Udine, Italy; "Santa Maria della Misericordia" University Hospital, Azienda Sanitaria Universitaria Friuli Centrale, Clinic of Obstetrics and Gynecology, Udine, Italy. FAU - Multinu, Francesco AU - Multinu F AD - European Institute of Oncology (IEO) IRCCS, Gynecologic Oncology Program, Milan, Italy; University of Milan, Department of Oncology and Hemato-Oncology, Milan, Italy; Early Career IJGC Editorial Board Member, International Journal of Gynecological Cancer. FAU - Aletti, Giovanni AU - Aletti G AD - European Institute of Oncology (IEO) IRCCS, Gynecologic Oncology Program, Milan, Italy; University of Milan, Department of Oncology and Hemato-Oncology, Milan, Italy; Editorial Board Member, International Journal of Gynecological Cancer. FAU - Biffi, Roberto AU - Biffi R AD - European Institute of Oncology (IEO) IRCCS, Gynecologic Oncology Program, Milan, Italy. FAU - Colombo, Nicoletta AU - Colombo N AD - European Institute of Oncology (IEO) IRCCS, Gynecologic Oncology Program, Milan, Italy. FAU - Maggioni, Angelo AU - Maggioni A AD - European Institute of Oncology (IEO) IRCCS, Gynecologic Oncology Program, Milan, Italy. LA - eng PT - Journal Article DEP - 20260923 PL - United States TA - Int J Gynecol Cancer JT - International journal of gynecological cancer : official journal of the International Gynecological Cancer Society JID - 9111626 SB - IM OTO - NOTNLM OT - Cervical Cancer OT - Pelvic Exenteration OT - Precision Oncology OT - Re-Irradiation OT - Targeted Therapy COIS- Declaration of Competing Interests Given their role as Editorial Board Members, G.A., D.C., and C.F. had no involvement in the peer review of this article and had no access to information regarding its peer review. Given their role as Early Career IJGC Editorial Board Members, G.C. and F.M. had no involvement in the peer review of this article and had no access to information regarding its peer review. N.A-R. reports grants or contracts paid to the institution from GRAIL. C.F. received honoraria for advisory boards from AstraZeneca, MSD, GSK, Medtronic, Oncoinvent, Eli Lilly, and AbbVie. The other authors have no conflicts of interest to declare. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:07 PHST- 2026/07/10 00:00 [received] PHST- 2026/09/09 00:00 [revised] PHST- 2026/09/12 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:07 [entrez] AID - S1048-891X(26)01160-6 [pii] AID - 10.1016/j.ijgc.2026.105629 [doi] PST - aheadofprint SO - Int J Gynecol Cancer. 2026 Sep 23;36(12):105629. doi: 10.1016/j.ijgc.2026.105629. PMID- 42950556 OWN - NLM STAT- Publisher LR - 20261028 IS - 1878-0407 (Electronic) IS - 1744-117X (Linking) VI - 61 DP - 2026 Oct 17 TI - Comparative genomic and transcriptomic dissection of protein phosphatase regulatory networks associated with spawning strategies in cyprinid fishes. PG - 102058 LID - S1744-117X(26)00317-5 [pii] LID - 10.1016/j.cbd.2026.102058 [doi] AB - The invasion of silver carp (Hypophthalmichthys molitrix) and bighead carp (H. nobilis) in North America exemplifies the severe ecological threats posed by invasive cyprinids. Their drifting (pelagic) eggs confer superior dispersal ability compared with the adhesive eggs of relatives such as common carp, yet the molecular basis remains poorly understood. Here, we hypothesize that evolutionary divergence in protein phosphatase pathways underlies these differences. By comparative genomics across 11 cypriniform species, we identified 169 single-copy orthologous genes (SCOGs) shared by all taxa. Species-specific positive-selection analyses detected up to 447 positively selected genes (PSGs) and up to 1345 rapidly evolving genes (REGs) among different lineages. Transcriptomic profiling of fertilized eggs from adhesive-egg (common carp, crucian carp) and drifting-egg (silver carp, bighead carp) species revealed distinct expression patterns. Integrative screening yielded 72 key genes, of which 11 are functionally linked to protein phosphatase/kinase regulation. Experimental validation indicates that adhesive-egg fishes exhibit predominant expression of the PP1 pathway, whereas drifting-egg fishes rely on the Mg(2+)/Mn(2+)-dependent PPM1D pathway. Drifting eggs rapidly absorb water and accumulate Mg(2+) within 30 min post-fertilization, which may promote PPM1D-mediated signaling to promote embryonic development, a trait that enhances survival and dispersal. Our findings reveal a molecular association between spawning type and invasive potential, and identify PPM1D and its Mg(2+) dependency as potential targets for invasion management, providing a theoretical foundation for managing the spread of invasive carps. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Xu, Chengbin AU - Xu C AD - Institute of International Rivers and Eco-security, Southwest United Graduate School, Yunnan University, Kunming, 650500, China; Key Laboratory of Green Prevention and Control of Tropical Agriculture and Forestry Disasters, School of Tropical Agriculture and Forestry, Hainan University, DanZhou, 571737, China. FAU - Ye, Baohui AU - Ye B AD - Key Laboratory of Green Prevention and Control of Tropical Agriculture and Forestry Disasters, School of Tropical Agriculture and Forestry, Hainan University, DanZhou, 571737, China. FAU - Liu, Shiying AU - Liu S AD - Key Laboratory of Green Prevention and Control of Tropical Agriculture and Forestry Disasters, School of Tropical Agriculture and Forestry, Hainan University, DanZhou, 571737, China. FAU - Liang, Yuanye AU - Liang Y AD - Key Laboratory of Green Prevention and Control of Tropical Agriculture and Forestry Disasters, School of Tropical Agriculture and Forestry, Hainan University, DanZhou, 571737, China. FAU - Guo, Xinglong AU - Guo X AD - Institute of International Rivers and Eco-security, Southwest United Graduate School, Yunnan University, Kunming, 650500, China. FAU - Zheng, Shicheng AU - Zheng S AD - Institute of International Rivers and Eco-security, Southwest United Graduate School, Yunnan University, Kunming, 650500, China. FAU - Shehzad, Muhammad Basit AU - Shehzad MB AD - Key Laboratory of Green Prevention and Control of Tropical Agriculture and Forestry Disasters, School of Tropical Agriculture and Forestry, Hainan University, DanZhou, 571737, China. FAU - Xie, Ping AU - Xie P AD - College of Life Sciences, Hebei University, Baoding, 071002, China; Institute for Ecological Research and Pollution Control of Plateau Lakes, School of Ecology and Environmental Sciences, Yunnan University, Kunming, 650500, China. Electronic address: xieping@ihb.ac.cn. FAU - Fan, Yongmei AU - Fan Y AD - Key Laboratory of Green Prevention and Control of Tropical Agriculture and Forestry Disasters, School of Tropical Agriculture and Forestry, Hainan University, DanZhou, 571737, China. Electronic address: yongmeifan@126.com. LA - eng PT - Journal Article DEP - 20261017 PL - Netherlands TA - Comp Biochem Physiol Part D Genomics Proteomics JT - Comparative biochemistry and physiology. Part D, Genomics & proteomics JID - 101270611 SB - IM OTO - NOTNLM OT - Biological invasion OT - Cyprinidae OT - Protein phosphatase (PPM1D) OT - Reproductive adaptation OT - Spawning strategy COIS- Declaration of competing interest No conflict of interest exits in the submission of this manuscript, and manuscript is approved by all authors for publication. I would like to declare on behalf of myself that the work described was original research that has not been published previously, and not under consideration for publication elsewhere, in whole or in part. All the authors listed have approved the manuscript that is enclosed.The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:07 PHST- 2026/08/06 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:07 [entrez] AID - S1744-117X(26)00317-5 [pii] AID - 10.1016/j.cbd.2026.102058 [doi] PST - aheadofprint SO - Comp Biochem Physiol Part D Genomics Proteomics. 2026 Oct 17;61:102058. doi: 10.1016/j.cbd.2026.102058. PMID- 42950551 OWN - NLM STAT- Publisher LR - 20261028 IS - 1477-2566 (Electronic) IS - 1465-3249 (Linking) VI - 29 IP - 1 DP - 2026 Jul 29 TI - Cell therapies: are we doing enough to protect patients and address the barriers to patient access? PG - 102959 LID - S1465-3249(26)00920-5 [pii] LID - 10.1016/j.jcyt.2026.102959 [doi] AB - This article is intended for cell therapy developers. It examines how unproven, unregulated cell therapies continue to pose significant risks to patients while simultaneously creating tangible obstacles for legitimate cell therapy developers. Specifically, direct-to-consumer clinics offering unproven and unregulated cell therapies expose vulnerable individuals to harm, erode public trust, and complicate the cell therapy clinical development landscape by competing unfairly with rigorously regulated cell therapies. This article examines these challenges and emphasizes the importance of distinguishing evidence-based cell therapy development from the unproven and unregulated practices of direct-to-consumer clinics. In the context of cell therapy, we provide an overview of regulated non-trial access pathways available to patients with serious or life-threatening conditions in the United States of America and European Union. These pathways, most notably the US Food and Drug Administration's Expanded Access Program and the European Union's Hospital Exemption, enable patient access to cell therapies outside of standard regulatory approval pathways when appropriate alternative therapies are unavailable or unsuitable. By operating within established regulatory frameworks, these pathways aim to balance patient access with safety oversight. Additionally, we address some of the practical challenges associated with conducting cell therapy clinical trials, which include trial design, endpoint selection, and patient expectation management. The viewpoints presented in the article aim to provide cell therapy developers with the framing and context needed to advocate for stronger regulatory coherence, protect legitimate cell therapy development pipelines, and ensure that patients access only safe and effective cell therapies. CI - Copyright (c) 2026 International Society for Cell & Gene Therapy. Published by Elsevier Inc. All rights reserved. FAU - Creane, Michael AU - Creane M AD - HAON Life Science, NovaUCD, Belfield Innovation Park, University College Dublin, Dublin, Ireland. FAU - Grilley, Bambi J AU - Grilley BJ AD - Department of Pediatrics, Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, Texas, USA. FAU - Forte, Miguel AU - Forte M AD - Kiji Therapeutics, Brussels, Belgium. FAU - Sanchez-Guijo, Fermin AU - Sanchez-Guijo F AD - University of Salamanca, IBSAL-University Hospital of Salamanca, Salamanca, Spain. FAU - Jorgensen, Christian AU - Jorgensen C AD - Institute for Regenerative Medicine and Biotherapy (IRMB), University Hospital of Montpellier, INSERM, Montpellier, France; Clinical Immunology and Osteoarticular Diseases Therapeutic Unit, Hopital Lapeyronie, Montpellier, France. FAU - Martin, Ivan AU - Martin I AD - Department of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland. FAU - Ikonomou, Laertis AU - Ikonomou L AD - Department of Oral Biology, University at Buffalo, The State University of New York, Buffalo, New York, USA; Cell, Gene and Tissue Engineering Center, University at Buffalo, The State University of New York, Buffalo, New York, USA. FAU - Viswanathan, Sowmya AU - Viswanathan S AD - Osteoarthritis Research Program, Division of Orthopaedic Surgery, Schroeder Arthritis Institute, University Health Network, Toronto, Ontario, Canada; Krembil Research Institute, University Health Network, Toronto, Ontario, Canada; Institute of Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada; Department of Medicine, Division of Hematology, University of Toronto, Toronto, Ontario, Canada. Electronic address: sowmya.viswanathan@uhn.ca. LA - eng PT - Journal Article DEP - 20260729 PL - England TA - Cytotherapy JT - Cytotherapy JID - 100895309 SB - IM OTO - NOTNLM OT - International Society for Cell & Gene Therapy OT - cell therapies OT - direct-to-consumer cell therapies OT - direct-to-consumer clinics OT - expanded access OT - hospital exemption OT - patient access OT - regulation OT - unproven cell therapies OT - unregulated cell therapies COIS- Declaration of competing interest BJG through QBRegulatory Consulting, LLC provides or has provided consulting services to AlloVir, Tessa Therapeutics, March Biosciences, Proxima Clinical Research, VVB Bio and Emory University. BJG is an equity holder in AlloVir and holder of stock options with March Bio and VVB Bio. BJG has received honoraria for speaking, presenting, lecturing, or as a grant or peer reviewer from CIRM, ISCT-CMaT, KIACET (Korea), IDRC (South Africa), JSRM (Japan), SITC and Elsevier. BJG is involved in projects that receive grant funding from CPRIT and the National Institutes of Health/National Cancer Institute. BJG received support for attending meetings/travel from ISCT, Emory University, and SITC. BJG sits on the ISCT Board of Directors and is the President Elect and serves leadership roles at ISCT. LI has written an expert report in a class action lawsuit filed against a business selling unproven "stem cell"-based interventions and wrote the report on a pro bono basis. SV has 60% ownership in a regulatory cell therapy consulting company. SV is the Vice-President of the ISCT North America Regional Executive Committee. MC is the Treasurer of the ISCT Europe Regional Executive Committee. MF sits on the ISCT Board of Directors and is the immediate past president. The remaining authors declare no conflict of interest. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:06 PHST- 2026/02/13 00:00 [received] PHST- 2026/07/01 00:00 [revised] PHST- 2026/07/20 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:06 [entrez] AID - S1465-3249(26)00920-5 [pii] AID - 10.1016/j.jcyt.2026.102959 [doi] PST - aheadofprint SO - Cytotherapy. 2026 Jul 29;29(1):102959. doi: 10.1016/j.jcyt.2026.102959. PMID- 42950549 OWN - NLM STAT- Publisher LR - 20261028 IS - 1878-7452 (Electronic) IS - 1878-7452 (Linking) VI - 83 IP - 12 DP - 2026 Sep 22 TI - Shifts in Scholarly Productivity Following Implementation of USMLE Step 1 Pass/Fail Reporting: Implications for General Surgery Residency Application. PG - 104219 LID - S1931-7204(26)00351-X [pii] LID - 10.1016/j.jsurg.2026.104219 [doi] AB - OBJECTIVE: Following the transition of the United States Medical Licensing Examination (USMLE) Step 1 from a 3-digit numerical score to pass/fail reporting in January 2022, the emphasis of scholarly productivity on residency applicant selection may have evolved. However, this change remains unclear. This study aims to evaluate changes in research productivity and scholarly activity (primary outcomes), as well as additional academic metrics (secondary outcomes), among general surgery residency applicants after the USMLE scoring. DESIGN: This is a retrospective single-institution study comparing Electronic Residency Application Service (ERAS) applications submitted to an academic general surgery residency program before (Group A: 2015-2021) and after (Group B: 2022-2024) implementation of pass/fail Step 1 reporting. Scholarly activity was categorized as (1) poster and oral presentations, (2) peer-reviewed journal articles and abstracts (published and unpublished), and (3) online publications. Groups were compared using Chi-square or Fisher's exact tests for categorical variables and parametric or nonparametric tests for continuous variables, as appropriate. SETTING: General surgery program at a single hybrid academic/community center. PARTICIPANTS: A total of 7,458 general surgery residency applicants were included: 5,503 (73.8%) in Group A and 1,955 (26.2%) in Group B. RESULTS: Among the 7458 general surgery applicants, 41% were female. Compared with Group A, applicants in Group B demonstrated significantly higher mean numbers of oral and poster presentations (5.44 vs 3.56, p < 0.001), journal articles and abstracts (4.95 vs 2.74, p < 0.001), and online publications (2.09 vs 0.25, p < 0.001). Group B also had higher mean (SD) USMLE Step 2 scores (243 [14] vs 237 [15], p < 0.001). CONCLUSIONS: Following the transition to pass/fail Step 1 reporting, general surgery residency applicants demonstrated increased research productivity and higher Step 2 scores, suggesting a potential shift toward strengthening objective academic metrics to maintain competitiveness. CI - Copyright (c) 2026 Association of Program Directors in Surgery. Published by Elsevier Inc. All rights reserved. FAU - Huhta, Nathaniel AU - Huhta N AD - Department of Surgery, Western Michigan University Homer Stryker M.D. School of Medicine, Kalamazoo, Michigan. Electronic address: nathaniel.huhta@wmed.edu. FAU - Moore, Megan AU - Moore M AD - Department of Surgery, Western Michigan University Homer Stryker M.D. School of Medicine, Kalamazoo, Michigan. FAU - Elian, Alain AU - Elian A AD - Department of Surgery, Western Michigan University Homer Stryker M.D. School of Medicine, Kalamazoo, Michigan. FAU - Shebrain, Saad AU - Shebrain S AD - Department of Surgery, Western Michigan University Homer Stryker M.D. School of Medicine, Kalamazoo, Michigan. LA - eng PT - Journal Article DEP - 20260922 PL - United States TA - J Surg Educ JT - Journal of surgical education JID - 101303204 SB - IM OTO - NOTNLM OT - ERAS applications OT - general surgery residency OT - research productivity OT - scholarly activity EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:06 PHST- 2026/04/26 00:00 [received] PHST- 2026/07/14 00:00 [revised] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:06 [entrez] AID - S1931-7204(26)00351-X [pii] AID - 10.1016/j.jsurg.2026.104219 [doi] PST - aheadofprint SO - J Surg Educ. 2026 Sep 22;83(12):104219. doi: 10.1016/j.jsurg.2026.104219. PMID- 42950540 OWN - NLM STAT- Publisher LR - 20261028 IS - 1938-0666 (Electronic) IS - 1526-8209 (Linking) VI - 26 IP - 11 DP - 2026 Sep 12 TI - Early On-Treatment Ultrasound Tumor Shrinkage and HER2-Positive Residual Invasive Disease After Neoadjuvant Therapy: A 2-Center Study. PG - 48-57 LID - S1526-8209(26)00169-2 [pii] LID - 10.1016/j.clbc.2026.09.006 [doi] AB - BACKGROUND: Residual invasive disease after neoadjuvant therapy (NAT) may differ in human epidermal growth factor receptor 2 (HER2) status from the pretreatment tumor. We evaluated whether early ultrasound response adds information for estimating HER2-positive residual invasive disease at surgery beyond baseline factors. PATIENTS AND METHODS: This retrospective 2-center study included women with nonmetastatic HER2-positive breast cancer treated with NAT and surgery. HER2-positive residual invasive disease was the primary endpoint; pathological complete response (pCR) and HER2-negative residual invasive disease formed the comparison group. The clinical model included nodal stage, estrogen receptor expression, pretreatment tumor size, baseline HER2 immunohistochemistry, treatment intensity, and timing of HER2-directed therapy. The dynamic model additionally included tumor shrinkage after 2 cycles. Performance was assessed by bootstrap correction, repeated cross-validation, and an external supportive cohort. RESULTS: Of 815 development-cohort patients, 224 had HER2-positive residual invasive disease; 26 of 83 external-cohort patients had this outcome. Median shrinkage was 40.8% for pCR, 27.8% for HER2-negative residual disease, and 18.5% for HER2-positive residual disease. Each 10-percentage-point greater shrinkage was associated with lower odds of HER2-positive residual invasive disease (odds ratio, 0.763; 95% confidence interval, 0.708-0.823). Repeated cross-validated area under the curve increased from 0.667 to 0.736; the external dynamic-model area under the curve was 0.874. Within residual disease, the increase was modest (0.634-0.656). CONCLUSION: Early ultrasound tumor shrinkage provided incremental risk information, but phenotype-specific discrimination was modest. Prospective evaluation is required before model estimates guide management. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Zhang, Chi AU - Zhang C AD - Department of Breast and Thyroid Surgery, Chongqing Key Laboratory of Molecular Oncology and Epigenetics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Zhou, Lin AU - Zhou L AD - Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China. FAU - Tang, Lingfeng AU - Tang L AD - Department of Breast and Thyroid Surgery, Chongqing Key Laboratory of Molecular Oncology and Epigenetics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Wang, Zijian AU - Wang Z AD - Department of Breast and Thyroid Surgery, Chongqing Key Laboratory of Molecular Oncology and Epigenetics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Li, Shunbo AU - Li S AD - Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China. FAU - Tang, Yimei AU - Tang Y AD - Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China. FAU - Tian, Chunxiang AU - Tian C AD - Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China. Electronic address: tiancx.cd@qq.com. FAU - Liu, Zeyu AU - Liu Z AD - Chengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China. Electronic address: 37781380@qq.com. FAU - Liu, Shengchun AU - Liu S AD - Department of Breast and Thyroid Surgery, Chongqing Key Laboratory of Molecular Oncology and Epigenetics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. Electronic address: liushengchun1968@163.com. LA - eng PT - Journal Article DEP - 20260912 PL - United States TA - Clin Breast Cancer JT - Clinical breast cancer JID - 100898731 SB - IM OTO - NOTNLM OT - HER2-positive breast cancer OT - Prediction model OT - Response assessment OT - Risk stratificatio OT - Ultrasonography COIS- Disclosure The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:06 PHST- 2026/07/18 00:00 [received] PHST- 2026/08/23 00:00 [revised] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:06 [entrez] AID - S1526-8209(26)00169-2 [pii] AID - 10.1016/j.clbc.2026.09.006 [doi] PST - aheadofprint SO - Clin Breast Cancer. 2026 Sep 12;26(11):48-57. doi: 10.1016/j.clbc.2026.09.006. PMID- 42950538 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-2448 (Electronic) IS - 0043-1354 (Linking) VI - 309 IP - Pt A DP - 2026 Oct 17 TI - Optimizing wastewater surveillance data by normalization to monitor pathogen circulation in dynamic populations for public health decision-making. PG - 127161 LID - S0043-1354(26)01833-6 [pii] LID - 10.1016/j.watres.2026.127161 [doi] AB - Wastewater surveillance is increasingly applied to monitor infectious disease trends. However, interpreting pathogen concentrations in wastewater is challenging because wastewater dilution and population size fluctuations can obscure true infection trends. To adjust for this, normalization using the human biomarkers Pepper Mild Mottle Virus (PMMoV), crAssphage, or flow-based approaches are commonly applied. Yet, the ability of these strategies to capture within-week and seasonal population changes, such as those caused by commuting and tourism, and their comparative effectiveness remains unclear. We conducted a longitudinal study at 59 wastewater treatment plants across the Netherlands, with varying levels of tourism and commuting. Between December 2020 and January 2021, and July 2023 to January 2024, 3829 wastewater samples were collected. SARS-CoV-2 (target pathogen), PMMoV, and crAssphage concentrations were quantified by RT-qPCR and flow data was retrieved from operational records. The different normalization strategies were evaluated by their effects on viral load outcomes in relation to COVID-19 case data and trend stability. PMMoV and crAssphage both reflect seasonal holiday-related dynamics and within-week changes due to commuting. Biomarker variability decreases as population size increases, indicating greater sensitivity in smaller catchments. Normalization of SARS-CoV-2 trends with biomarkers or flow leads to reduced residual variance, facilitating trend recognition. Normalization using human biomarkers or flow can substantially improve the reliability of wastewater-based pathogen surveillance, especially in areas with dynamic populations. These findings guide selection of normalization strategies to improve detection of pathogen trends, thereby enhancing the value of wastewater surveillance for public health decision-making. CI - Copyright (c) 2026 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - van der Drift, Anne-Merel R AU - van der Drift AR AD - Centre for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM), Antonie van Leeuwenhoeklaan 9, Bilthoven, 3721 MA, the Netherlands; Institute for Risk Assessment Science (IRAS), Utrecht University (UU), Yalelaan 2, Utrecht, 3584 CM, the Netherlands. Electronic address: anne-merel.van.der.drift@rivm.nl. FAU - Senyer, Merve AU - Senyer M AD - Centre for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM), Antonie van Leeuwenhoeklaan 9, Bilthoven, 3721 MA, the Netherlands. FAU - Agrandi, Sharaf AU - Agrandi S AD - Centre for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM), Antonie van Leeuwenhoeklaan 9, Bilthoven, 3721 MA, the Netherlands. FAU - Hetebrij, Wouter A AU - Hetebrij WA AD - Centre for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM), Antonie van Leeuwenhoeklaan 9, Bilthoven, 3721 MA, the Netherlands. FAU - Lodder, Willemijn J AU - Lodder WJ AD - Centre for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM), Antonie van Leeuwenhoeklaan 9, Bilthoven, 3721 MA, the Netherlands. FAU - de Jonge, Eline AU - de Jonge E AD - Centre for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM), Antonie van Leeuwenhoeklaan 9, Bilthoven, 3721 MA, the Netherlands. FAU - Carstens, Gesa AU - Carstens G AD - Centre for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM), Antonie van Leeuwenhoeklaan 9, Bilthoven, 3721 MA, the Netherlands. FAU - de Roda Husman, Ana Maria AU - de Roda Husman AM AD - Centre for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM), Antonie van Leeuwenhoeklaan 9, Bilthoven, 3721 MA, the Netherlands; Institute for Risk Assessment Science (IRAS), Utrecht University (UU), Yalelaan 2, Utrecht, 3584 CM, the Netherlands. FAU - van der Beek, Rudolf F H J AU - van der Beek RFHJ AD - Centre for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM), Antonie van Leeuwenhoeklaan 9, Bilthoven, 3721 MA, the Netherlands. FAU - Nagelkerke, Erwin AU - Nagelkerke E AD - Centre for Infectious Disease Control (CIb), National Institute for Public Health and the Environment (RIVM), Antonie van Leeuwenhoeklaan 9, Bilthoven, 3721 MA, the Netherlands. LA - eng PT - Journal Article DEP - 20261017 PL - England TA - Water Res JT - Water research JID - 0105072 SB - IM OTO - NOTNLM OT - Commuters OT - Sewage OT - Storage effects OT - Tourism OT - Wastewater-based epidemiology COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:06 PHST- 2026/07/29 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:06 [entrez] AID - S0043-1354(26)01833-6 [pii] AID - 10.1016/j.watres.2026.127161 [doi] PST - aheadofprint SO - Water Res. 2026 Oct 17;309(Pt A):127161. doi: 10.1016/j.watres.2026.127161. PMID- 42950536 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-2448 (Electronic) IS - 0043-1354 (Linking) VI - 309 IP - Pt A DP - 2026 Oct 23 TI - Coupled extraction and spontaneous room-temperature degradation of haloacetic acids in a hydrophobic deep eutectic solvent. PG - 127190 LID - S0043-1354(26)01862-2 [pii] LID - 10.1016/j.watres.2026.127190 [doi] AB - Haloacetic acids (HAAs) are persistent, highly mobile disinfection byproducts in drinking water distribution systems and disinfected wastewater. Their small size and hydrophilic nature make the removal and degradation in water difficult. Here, we examine a hydrophobic deep eutectic solvent (HDES) as an integrated extraction and degradation phase for representative HAAs. The HDES efficiently partitioned mono-, di-, and trihalogenated HAAs from clean water and wastewater effluent into a distinct nonaqueous phase. Whereas degradation of PFAS in the same HDES system required heating, the enriched HAAs underwent extensive room-temperature C-halogen and C-C bond cleavage in basic HDES without external energy input, a reactivity inaccessible in water. Product distributions revealed halogenation-dependent pathway selection: mono- and dihalogenated substrates followed C2-preserving hydrolytic dehalogenation, whereas trihalogenated substrates shifted toward decarboxylation-derived C1 fragmentation. Comparative density functional theory calculations showed that HDES lowers activation barriers for competing dehalogenation and decarboxylation initiation steps relative to water and revealed an alpha-halogenation-dependent crossover in pathway accessibility. Five sequential extraction/destruction cycles in wastewater effluent retained both separation and reaction functionality. By uniting extraction and room-temperature destruction in a single engineered phase, this work advances solvent-phase engineering as a strategy for contaminants that are difficult to both remove and degrade in water. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Fan, Zixi AU - Fan Z AD - Environmental Engineering Program, Department of Chemical and Environmental Engineering, University of Cincinnati, Cincinnati, Ohio, 45221, United States; Department of Civil, Materials, and Environmental Engineering, University of Illinois Chicago, Chicago, Illinois, 60607, United States. FAU - Mendez, Christian M AU - Mendez CM AD - Environmental Engineering Program, Department of Chemical and Environmental Engineering, University of Cincinnati, Cincinnati, Ohio, 45221, United States. FAU - Bentel, Michael J AU - Bentel MJ AD - Department of Civil, Materials, and Environmental Engineering, University of Illinois Chicago, Chicago, Illinois, 60607, United States. FAU - Niu, Xi-Zhi AU - Niu XZ AD - Environmental Engineering Program, Department of Chemical and Environmental Engineering, University of Cincinnati, Cincinnati, Ohio, 45221, United States. Electronic address: xi-zhi.niu@uc.edu. LA - eng PT - Journal Article DEP - 20261023 PL - England TA - Water Res JT - Water research JID - 0105072 SB - IM OTO - NOTNLM OT - Decarboxylation OT - Dehalogenation OT - Disinfection byproducts OT - Haloacetic acids OT - Hydrophobic deep eutectic solvents OT - Integrated extraction and degradation COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:06 PHST- 2026/07/02 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:06 [entrez] AID - S0043-1354(26)01862-2 [pii] AID - 10.1016/j.watres.2026.127190 [doi] PST - aheadofprint SO - Water Res. 2026 Oct 23;309(Pt A):127190. doi: 10.1016/j.watres.2026.127190. PMID- 42950505 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-3778 (Electronic) IS - 0021-9673 (Linking) VI - 1790 DP - 2026 Oct 21 TI - The challenge of identification in the analysis of per- and polyfluoroalkyl substances in food: risks of false positive by liquid chromatography tandem mass spectrometry. PG - 467563 LID - S0021-9673(26)00889-7 [pii] LID - 10.1016/j.chroma.2026.467563 [doi] AB - Reliable identification of per- and polyfluoroalkyl substances (PFAS) in complex food matrices remains analytically challenging, particularly for compounds exhibiting limited fragmentation in low-resolution mass spectrometry. Current identification criteria require at least two ion transitions; however, for short-chain PFAS, the second transition is often a precursor-->precursor signal or a non-specific fragment, which may not provide structural confirmation. In this study, the risk of false positive identification is demonstrated using two case studies. First, apparent detection of perfluoropentanoic acid (PFPeA) in soluble coffee was shown to be an analytical artefact after modification of chromatographic conditions revealed a co-eluting interference. Second, analysis of N-methyl perfluorooctane sulfonamidoethanol (N-MeFOSE) in pizza samples demonstrated that transitions based on ammonium acetate adducts and non-specific fragments (m/z 59) do not provide confirmation. In both cases, alternative approaches (LC-MS/MS alternative chromatographic conditions and GC-MS/MS) demonstrated the absence of the target analytes. These findings highlight that in cases where only a single or non-selective MRM transition is available, the use of an alternative analytical approach such as GC-MS/MS, LC-MS/MS with modified chromatographic conditions, or LCHRMS should be considered to ensure reliable confirmation. Verification through spiking experiments may reveal the presence of co-eluting matrix interferences, and a critical evaluation of suspect results prior to reporting is essential to prevent misidentification of PFAS and the generation of false positive results. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Theurillat, Xanthippe AU - Theurillat X AD - Societe des Produits Nestle SA. - Nestle Research Lausanne, Vers-chez-les-Blanc, 1000 Lausanne 26, Switzerland. Electronic address: xanthippi.theurillat@rdls.nestle.com. FAU - Eriksen, Bjorn AU - Eriksen B AD - Societe des Produits Nestle SA. - Nestle Research Lausanne, Vers-chez-les-Blanc, 1000 Lausanne 26, Switzerland. Electronic address: bjorn.eriksen@rdls.nestle.com. FAU - Delatour, Thierry AU - Delatour T AD - Societe des Produits Nestle SA. - Nestle Research Lausanne, Vers-chez-les-Blanc, 1000 Lausanne 26, Switzerland. Electronic address: thierry.delatour@rdls.nestle.com. LA - eng PT - Journal Article DEP - 20261021 PL - Netherlands TA - J Chromatogr A JT - Journal of chromatography. A JID - 9318488 SB - IM OTO - NOTNLM OT - Identification / confirmation OT - LC-MS/MS OT - Per- and polyfluoroalkyl substances OT - Risk of false positive detection COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:05 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:05 [entrez] AID - S0021-9673(26)00889-7 [pii] AID - 10.1016/j.chroma.2026.467563 [doi] PST - aheadofprint SO - J Chromatogr A. 2026 Oct 21;1790:467563. doi: 10.1016/j.chroma.2026.467563. PMID- 42950503 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-3778 (Electronic) IS - 0021-9673 (Linking) VI - 1790 DP - 2026 Oct 19 TI - Fast HPLC separation of rare earths with direct VUV detection and superficially porous particle-based stationary phases. PG - 467561 LID - S0021-9673(26)00887-3 [pii] LID - 10.1016/j.chroma.2026.467561 [doi] AB - A liquid chromatography-vacuum ultraviolet detection (LC-VUV) method was developed for isocratic rare-earth element separation and direct detection using aqueous mobile phases containing alpha-hydroxyisobutyric acid (HIBA) and gamma-aminopropylsilane-halodecane silica stationary phases. Chlorodecane- and iododecane-modified fully porous and superficially porous gamma-APS columns were evaluated to determine how stationary-phase chemistry, stationary-phase format, HIBA concentration, and pH affect retention. Retention studies and HIBA-free control injections support a coupled mechanism in which Ln-HIBA species interact with the modified stationary phase, rather than simple retention of free HIBA or uncomplexed hydrated Ln(III) ions. Iododecane phases produced stronger retention, especially for heavier lanthanides, while SPP columns provided faster separations. LC-VUV enabled direct detection of La-Lu, excluding Pm, under isocratic aqueous HIBA conditions. Cl-SPP stationary phase separated most lanthanides in <4 min, while I-SPP expanded the retention window for middle and heavy lanthanides in <10 min. Dy/Y remained the main unresolved pair. VUV provided measurable LODs for all evaluated REEs, including Sc, Y, La, Gd, Tb, Yb, and Lu, which were not detected by UV-Vis under the tested conditions. The lowest VUV LODs ranged from 0.6 ppm for Ce to 29 ppm for Lu. VUV spectra provided complementary information for selected elements and matrix components, and a real Nd-Fe-B magnet digest demonstrated baseline separation of Fe and Nd. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Salehi, Reza AU - Salehi R AD - The University of Texas at Arlington, Chemistry Department, 700 Planetarium Place, CPB110, Arlington, TX, 76019, USA. FAU - Olds, Maria AU - Olds M AD - The University of Texas at Arlington, Chemistry Department, 700 Planetarium Place, CPB110, Arlington, TX, 76019, USA. FAU - Aslani, Saba AU - Aslani S AD - The University of Texas at Arlington, Chemistry Department, 700 Planetarium Place, CPB110, Arlington, TX, 76019, USA. FAU - Dombrowski, Annika AU - Dombrowski A AD - VUV Analytics, 1500 Arrow Point Drive, Suite 805, Building 8, Cedar Park, TX, 78613, USA. FAU - Armstrong, Daniel W AU - Armstrong DW AD - The University of Texas at Arlington, Chemistry Department, 700 Planetarium Place, CPB110, Arlington, TX, 76019, USA. Electronic address: sec4dwa@uta.edu. LA - eng PT - Journal Article DEP - 20261019 PL - Netherlands TA - J Chromatogr A JT - Journal of chromatography. A JID - 9318488 SB - IM OTO - NOTNLM OT - Custom-made stationary phases OT - Direct detection OT - HIBA OT - Rare-earth elements OT - Vacuum ultraviolet detection COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Dr. Daniel W. Armstrong has a potential research conflict of interest due to a financial interest with the company AZYP, LLC. A management plan has been created to preserve objectively in research in accordance with UTA policy. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:05 PHST- 2026/05/21 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:05 [entrez] AID - S0021-9673(26)00887-3 [pii] AID - 10.1016/j.chroma.2026.467561 [doi] PST - aheadofprint SO - J Chromatogr A. 2026 Oct 19;1790:467561. doi: 10.1016/j.chroma.2026.467561. PMID- 42950492 OWN - NLM STAT- Publisher LR - 20261028 IS - 1532-2157 (Electronic) IS - 0748-7983 (Linking) VI - 53 IP - 1 DP - 2026 Oct 20 TI - A simple three-variable triage rule using PR Allred score, standardized digital Ki-67, and histologic grade to identify an Oncotype DX recurrence score of 25 or less in HR+/HER2- early breast cancer. PG - 112329 LID - S0748-7983(26)00948-0 [pii] LID - 10.1016/j.ejso.2026.112329 [doi] AB - INTRODUCTION: The 21-gene Oncotype DX Recurrence Score (RS) is inaccessible in many settings due to cost. This study developed a clinicopathologic nomogram and a simple clinical rule to identify patients likely to have an RS /=50% coverage; internal validation used bootstrap resampling that repeated the threshold search. RESULTS: PR Allred score, Ki-67 index, and histologic grade were independent predictors of RS /=6, Ki-67 Over a century of orthodontic research: A bibliometric and scientometric analysis of growth, citation impact, and structural evolution. PG - 101257 LID - S1761-7227(26)00124-5 [pii] LID - 10.1016/j.ortho.2026.101257 [doi] AB - INTRODUCTION: Orthodontic research has grown for more than a century, but its evidence base has not been examined across its full history. We evaluated its evolution in publication growth, citation impact, journal distribution, geographic contribution, and international collaboration. METHODS: We froze an OpenAlex topic-indexed corpus ("Orthodontics and Dentofacial Orthopedics") on December 31, 2025, and, after removing pre-1900 metadata artefacts, analysed 46,265 records from 1900 to 2025; in a two-rater manual check of a random sample, 92.4% of records were genuinely orthodontic (precision; recall not assessed). We assessed growth, structural transitions, citation inequality, field-weighted citation impact (FWCI), publishing concentration, country-level output, language, and collaboration. RESULTS: Output accelerated after a structural changepoint in 1996: the absolute slope of annual output (records added per year) increased more than 25-fold while the proportional growth rate remained broadly stable; 86.5% of the corpus dates from 1996 onward. Citation impact was skewed (median 2 citations, 35.7% uncited, Gini coefficient 0.794). Annual mean FWCI exceeded 1.0 from 2022 onward, although over short citation windows. Among venue-resolved records, the annual Herfindahl-Hirschman Index fell from 920 in 1990 to 97 in 2024, and 95% of the 2024 output was spread across 460 venues (1455 across all years). Among the 62.5% of records with resolved affiliations, the United States led cumulative output under whole counting (5265 papers), followed by Brazil (3360) and India (2757); India led annual output in 2021-2024. Internationally co-authored papers were associated with higher citation impact than single-country papers in every period (median FWCI 0.90 versus 0.47). CONCLUSION: Orthodontic research is now predominantly post-1990s, globally redistributed, dispersed across a large journal ecosystem, and highly asymmetric in citation impact. These results provide a quantitative baseline; they are consistent with, but do not by themselves establish, implications for literature retrieval, journal selection, and collaborative research strategy. CI - Copyright (c) 2026 The Authors. Published by Elsevier Masson SAS.. All rights reserved. FAU - Zecca, Piero Antonio AU - Zecca PA AD - University of Insubria, Department of Medicine and Technological Innovation, Via Monte Generoso 71, 21100 Varese, Italy. Electronic address: pieroantonio.zecca@uninsubria.it. FAU - Serafin, Marco AU - Serafin M AD - University of Milan, Department of Biomedical, Surgical, and Dental Sciences, Milan, Italy. FAU - Caprioglio, Alberto AU - Caprioglio A AD - University of Milan, Department of Biomedical, Surgical, and Dental Sciences, Milan, Italy; Ospedale Maggiore Policlinico, IRCCS Ca' Granda, Milan, Italy. LA - eng PT - Journal Article DEP - 20261028 PL - France TA - Int Orthod JT - International orthodontics JID - 101184882 SB - IM OTO - NOTNLM OT - Bibliometrics OT - Citation analysis OT - Geographic distribution OT - Orthodontics OT - Publication trends OT - Scientific output COIS- Disclosure of interest The authors declare that they have no competing interest. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:04 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:04 [entrez] AID - S1761-7227(26)00124-5 [pii] AID - 10.1016/j.ortho.2026.101257 [doi] PST - aheadofprint SO - Int Orthod. 2026 Oct 28;25(1):101257. doi: 10.1016/j.ortho.2026.101257. PMID- 42950434 OWN - NLM STAT- Publisher LR - 20261028 IS - 1540-1413 (Electronic) IS - 1540-1405 (Linking) DP - 2026 Oct 28 TI - Optimizing Breast Cancer Surgery Recovery: A Real-World Comparative Effectiveness Study of Prehabilitation and Delayed Rehabilitation Care. LID - e267051 [pii] LID - 10.6004/jnccn.2026.7051 [doi] AB - BACKGROUND: Prehabilitation is recommended to reduce surgery-related functional decline but is not routinely integrated into presurgical breast cancer care, often resulting in rehabilitation being delayed until after surgery. We conducted a retrospective comparative effectiveness study evaluating outcomes associated with real-world prehabilitation versus delayed rehabilitation care models. METHODS: Women who began rehabilitation before (prehabilitation; n=416) or after breast cancer surgery (delayed rehabilitation; n=232) were identified from the electronic medical record. Upper extremity function and health-related quality of life (HRQoL) outcomes were extracted presurgery, postsurgery, and at final evaluation. Adjusted linear mixed-effect models examined change over time and between-group differences, and logistic regression estimated likelihood of achieving normal limits. Analyses were conducted overall and stratified by surgery type. Rehabilitation utilization and recovery to baseline levels among patients who received prehabilitation were summarized descriptively. RESULTS: Prehabilitation was associated with superior postsurgery function and HRQoL, as well as higher odds of achieving normal limits, compared with delayed rehabilitation. Both groups improved during postoperative rehabilitation; however, functional outcomes and strength remained superior among patients who received prehabilitation at final evaluation. Benefits of prehabilitation were most pronounced among women undergoing mastectomy or bilateral mastectomy, although meaningful benefits were also observed following lumpectomy. All patients who received prehabilitation recovered to baseline levels on at least one outcome. Across the perioperative period, patients who received prehabilitation attended fewer visits than those who received delayed rehabilitation (median, 5 vs 7; P=.008). CONCLUSIONS: Both prehabilitation and delayed rehabilitation supported recovery of upper extremity functioning and HRQoL following breast cancer surgery. However, prehabilitation may represent a more effective and efficient care model-particularly for women undergoing more extensive surgical procedures-achieving comparable or better outcomes with fewer visits. These findings support integration of prehabilitation into breast cancer surgical care pathways. FAU - Wood, Kelley C AU - Wood KC AD - 1ReVital Cancer Rehabilitation, Select Medical, Mechanicsburg, PA. FAU - Bertram, Jessica J AU - Bertram JJ AD - 1ReVital Cancer Rehabilitation, Select Medical, Mechanicsburg, PA. AD - 2Baylor Scott & White Institute for Rehabilitation, Dallas, TX. FAU - Hodges, Amanda AU - Hodges A AD - 1ReVital Cancer Rehabilitation, Select Medical, Mechanicsburg, PA. FAU - Godbole, Nikita AU - Godbole N AD - 3Emory Healthcare, Atlanta, GA. FAU - Brizeno, Vanessa G AU - Brizeno VG AD - 4Baylor Scott & White Institute for Rehabilitation, Austin, TX. FAU - Perchaluk, Jessie AU - Perchaluk J AD - 5NovaCare Rehabilitation, Southampton, PA. FAU - Ng, Peter C AU - Ng PC AD - 6Kessler Rehabilitation Center, Monroe Township, NJ. FAU - Andris, Michelle AU - Andris M AD - 7Select Physical Therapy, Normandy Park, WA. FAU - Rice, Julie AU - Rice J AD - 8Select Physical Therapy, Puyallup, WA. FAU - Thompson, Kelsey N AU - Thompson KN AD - 2Baylor Scott & White Institute for Rehabilitation, Dallas, TX. FAU - Meehan, Deanna AU - Meehan D AD - 9Kessler Rehabilitation Center, Edison, NJ. FAU - Redfern, Megan AU - Redfern M AD - 10Select Physical Therapy, Port Orchard, WA. FAU - Manvi, Sruti AU - Manvi S AD - 2Baylor Scott & White Institute for Rehabilitation, Dallas, TX. FAU - Canavan, Lynn AU - Canavan L AD - 11Texas Breast Specialists, McKinney, TX. FAU - Pergolotti, Mackenzi AU - Pergolotti M AD - 1ReVital Cancer Rehabilitation, Select Medical, Mechanicsburg, PA. AD - 12Department of Occupational Therapy, Colorado State University, Fort Collins, CO. AD - 13Department of Occupational Science and Occupational Therapy, University of North Carolina, Chapel Hill, NC. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Natl Compr Canc Netw JT - Journal of the National Comprehensive Cancer Network : JNCCN JID - 101162515 SB - IM OTO - NOTNLM OT - Perioperative Care OT - Prehabilitation OT - Quality of Life OT - Recovery of Function OT - Upper Extremity EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:03 PHST- 2026/01/06 00:00 [received] PHST- 2026/05/27 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:03 [entrez] AID - e267051 [pii] AID - 10.6004/jnccn.2026.7051 [doi] PST - aheadofprint SO - J Natl Compr Canc Netw. 2026 Oct 28:e267051. doi: 10.6004/jnccn.2026.7051. PMID- 42950433 OWN - NLM STAT- Publisher LR - 20261028 IS - 1361-6579 (Electronic) IS - 0967-3334 (Linking) DP - 2026 Oct 28 TI - Cross-Database Generalization of a Single-Feature Threshold Model for Congestive Heart Failure Detection from Short-Term Heart Rate Variability. LID - 10.1088/1361-6579/aebae2 [doi] AB - Objective To evaluate the transferability of interpretable single-feature threshold models across the five evaluated databases for congestive heart failure (CHF) detection from short-term heart rate variability (HRV), and to compare their performance with optimized multi-feature classifiers. Approach Twenty HRV features from 60-second inter-beat interval windows in five databases were evaluated using leave-one-database-pair-out nested cross-validation with six outer-test sets and their corresponding outer-development sets. Within each outer-development set, directions and thresholds were fitted on inner-train sets, and features were selected according to mean inner-validation F1 score. The selected model was refitted on the complete outer-development set. The most frequently selected feature was further evaluated across the six outer-test sets with separately fitted directions and thresholds. Eight multi-feature classifiers were optimized and evaluated using the same data splits. Performance was assessed using record-balanced window-level metrics and database-stratified recording-level bootstrap confidence intervals. Main results The short-term detrended fluctuation analysis scaling exponent (f14) was selected most frequently. The f14 threshold model achieved the highest mean test AUC (0.881 +/- 0.038), F1 score (0.718 +/- 0.079), and sensitivity among the evaluated single-feature and multi-feature models. Among the eight multi-feature classifiers, Random Forest achieved the highest mean test AUC (0.858 +/- 0.067). Significance An interpretable single-feature threshold model showed strong CHF-Normal Sinus Rhythm (NSR) discrimination in the five evaluated databases. f14 was the strongest candidate in this evaluation, although its thresholds varied across outer-development sets and its clinical specificity requires further validation. CI - (c) 2026 Institute of Physics and Engineering in Medicine. All rights, including for text and data mining, AI training, and similar technologies, are reserved. FAU - Song, Yongjun AU - Song Y AUID- ORCID: 0000-0001-8929-3564 AD - School of Computer Science and Engineering, Guangzhou Institute of Science and Technology, No. 138, Xing Tai San Road, Taihe Town, Baiyun District, Guangzhou, Guangdong, 510540, China. FAU - Wu, Bangyu AU - Wu B AD - School of Computer Science and Engineering, Guangzhou Institute of Science and Technology, No. 138, Xing Tai San Road, Taihe Town, Baiyun District, Guangzhou, Guangdong, 510540, China. FAU - Song, Yingying AU - Song Y AD - School of Computer Science and Engineering, Guangzhou Institute of Science and Technology, No. 138, Xing Tai San Road, Taihe Town, Baiyun District, Guangzhou, Guangzhou, Guangdong, 510540, China. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Physiol Meas JT - Physiological measurement JID - 9306921 SB - IM OTO - NOTNLM OT - Congestive heart failure OT - Cross-database validation OT - Heart rate variability OT - Mobile health OT - Short-term detrended fluctuation analysis OT - Wearable monitoring EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:03 PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:03 [entrez] AID - 10.1088/1361-6579/aebae2 [doi] PST - aheadofprint SO - Physiol Meas. 2026 Oct 28. doi: 10.1088/1361-6579/aebae2. PMID- 42950427 OWN - NLM STAT- Publisher LR - 20261028 IS - 1943-5681 (Electronic) IS - 0002-9645 (Linking) DP - 2026 Oct 28 TI - Evaluation of the sterility and clinical use of a disposable spray cap for ophthalmic medication delivery in equine patients. PG - 1-8 LID - 10.2460/ajvr.26.06.0263 [doi] AB - OBJECTIVE: To evaluate the sterility of a commercially available disposable spray cap in an equine barn environment and assess its clinical use. METHODS: This pilot study included an ex vivo microbiological study and an in vivo usability study. Ex vivo study: diclofenac 0.1% ophthalmic solution and neomycin-polymyxin B-dexamethasone 0.1% ophthalmic suspension were evaluated using either a traditional dropper or spray cap treatment method. Bottles were stored in an equine veterinary hospital barn and dispensed every 12 hours. Samples collected on day 0 and the final study day were analyzed for microbial contamination with next-generation DNA sequencing. In vivo usability study: client-owned horses were enrolled, and owners administered ophthalmic medications with the spray cap. A client survey evaluated owner satisfaction with the spray cap method. RESULTS: Next-generation DNA sequencing detected no microbial contamination in any of the ophthalmic samples. Five horses were enrolled in the in vivo usability study, and 3 horses seemed to tolerate the spray cap very well, with the highest mean score for a horse of 4.9/5. Two horses did not seem to tolerate the spray cap, with the lowest mean score of 2.0/5. Audible spraying sound produced during activation of the device was reported as the biggest problem. CONCLUSIONS: The spray cap remained sterile in a barn environment and demonstrated favorable clinical use for some horses. Horse tolerance was variable depending on their temperament. CLINICAL RELEVANCE: Disposable spray cap devices may represent a practical alternative for topical ophthalmic medication administration in selected equine patients and may also have applications for other species in veterinary medicine. FAU - Raphtis, Vanessa AU - Raphtis V AUID- ORCID: 0009-0008-5941-7974 FAU - Barr, Erin AU - Barr E FAU - Henriksen, Michala de Linde AU - Henriksen ML AUID- ORCID: 0000-0002-7901-7790 LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Am J Vet Res JT - American journal of veterinary research JID - 0375011 SB - IM OTO - NOTNLM OT - alternative treatment methods OT - microbiology OT - next-generation DNA sequencing OT - ocular drug delivery OT - ophthalmology EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:03 PHST- 2026/06/15 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:03 [entrez] AID - 10.2460/ajvr.26.06.0263 [doi] PST - aheadofprint SO - Am J Vet Res. 2026 Oct 28:1-8. doi: 10.2460/ajvr.26.06.0263. PMID- 42950425 OWN - NLM STAT- Publisher LR - 20261028 IS - 1943-569X (Electronic) IS - 0003-1488 (Linking) DP - 2026 Oct 28 TI - Use of 2.5% polyacrylamide hydrogel mitigates joint capsule stiffness and cartilage degradation in experimental equine osteoarthritis. PG - 1-11 LID - 10.2460/javma.26.04.0315 [doi] AB - OBJECTIVE: To evaluate the symptom- and disease-modifying effects of injectable 2.5% polyacrylamide gel (iPAAG) in an experimental model of equine osteoarthritis (OA). METHODS: 16 horses underwent osteochondral fragment creation (day 0) in 1 randomly-selected middle carpal joint (MCJ). On day 14, the MCJ was injected with 2 mL of phosphate-buffered saline (n = 8) or 2.5% iPAAG (8); high-speed treadmill exercise commenced on day 16 to induce OA. Clinical parameters (lameness, joint effusion, range of motion, flexion response) and synovial fluid were collected weekly and biweekly, respectively. Radiographs were obtained before surgery, at day 14, and at day 70. Postmortem evaluation (day 70) included gross, arthroscopic, and histopathologic evaluation of the MCJs and measurement of joint capsule stiffness. RESULTS: There were no significant differences in clinical parameters or endpoint arthroscopic, macroscopic, or synovial membrane histopathology scores between groups. In 2.5% iPAAG-treated limbs, the third carpal bone had significantly lower (better) total articular cartilage histology scores (6.38 +/- 4.10 vs 11.25 +/- 4.20) and proteoglycan staining scores of the intermediate articular cartilage layer (0.38 +/- 0.74 vs 1.88 +/- 1.25) compared to saline-treated OA joints. Saline-treated OA joints had significantly increased joint capsule stiffness versus contralateral sham-operated MCJs (1,824 +/- 1,105 vs 1,002 +/- 495 Pa), whereas 2.5% iPAAG-treated joints did not (1,099 +/- 647 vs 1,184 +/- 504 Pa). CONCLUSIONS: Treatment with 2.5% iPAAG showed some ability to mitigate the negative effects of OA on articular cartilage. Treatment also resulted in retention of normal joint capsule elasticity during the progression of OA. CLINICAL RELEVANCE: Treatment with 2.5% iPAAG may result in retention of normal joint capsule elasticity in horses with naturally occurring OA. FAU - Contino, Erin K AU - Contino EK AUID- ORCID: 0000-0002-1756-510X AD - 1Orthopaedic Research Center, Translational Medicine Institute, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO. FAU - AbuAlia, Mohammed J AU - AbuAlia MJ AUID- ORCID: 0000-0001-7643-2174 AD - 2Department of Orthopedic Surgery, Rush University Medical Center, Chicago, IL. FAU - Nelson, Brad B AU - Nelson BB AUID- ORCID: 0000-0002-0205-418X AD - 1Orthopaedic Research Center, Translational Medicine Institute, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO. FAU - Goodrich, Laurie R AU - Goodrich LR AUID- ORCID: 0000-0002-8010-4429 AD - 1Orthopaedic Research Center, Translational Medicine Institute, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO. FAU - Barrett, Myra F AU - Barrett MF AUID- ORCID: 0000-0001-5060-6009 AD - 1Orthopaedic Research Center, Translational Medicine Institute, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO. FAU - King, Melissa R AU - King MR AUID- ORCID: 0000-0002-1203-3059 AD - 1Orthopaedic Research Center, Translational Medicine Institute, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO. FAU - Pezzanite, Lynn M AU - Pezzanite LM AUID- ORCID: 0000-0003-4990-5006 AD - 1Orthopaedic Research Center, Translational Medicine Institute, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO. FAU - McIlwraith, C Wayne AU - McIlwraith CW AUID- ORCID: 0000-0002-7302-2265 AD - 1Orthopaedic Research Center, Translational Medicine Institute, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO. FAU - Wimmer, Markus A AU - Wimmer MA AUID- ORCID: 0000-0001-6169-3873 AD - 2Department of Orthopedic Surgery, Rush University Medical Center, Chicago, IL. FAU - Seabaugh, Kathryn A AU - Seabaugh KA AUID- ORCID: 0000-0002-3088-8827 AD - 1Orthopaedic Research Center, Translational Medicine Institute, Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Am Vet Med Assoc JT - Journal of the American Veterinary Medical Association JID - 7503067 SB - IM OTO - NOTNLM OT - equine OT - intra-articular OT - joint capsule OT - osteoarthritis OT - polyacrylamide gel EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:03 PHST- 2026/05/05 00:00 [received] PHST- 2026/09/08 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:03 [entrez] AID - 10.2460/javma.26.04.0315 [doi] PST - aheadofprint SO - J Am Vet Med Assoc. 2026 Oct 28:1-11. doi: 10.2460/javma.26.04.0315. PMID- 42950424 OWN - NLM STAT- Publisher LR - 20261028 IS - 1361-6579 (Electronic) IS - 0967-3334 (Linking) DP - 2026 Oct 28 TI - Measured, not labelled: un-analyzable input versus misclassification in single-lead wearable ECG. LID - 10.1088/1361-6579/aebae3 [doi] AB - Wearable single-lead ECG has expanded arrhythmia screening, but many recordings are inconclusive, and each corpus defines that word differently. We ask how much deployment error is un-analyzable input rather than misclassification when analyzability is measured with one instrument. Approach. We evaluate three diagnostic databases in-domain and leave-dataset-out under a strict loss counting windows carrying each corpus's inconclusive label as errors unless deferred. We score analyzability with an unsupervised morphological criterion calibrated against three experts on free-living ECG. Reference unreadability is sparse and imbalanced: only seven of 15 subjects contain an unreadable window and one supplies 88% of all unreadable windows. Thresholded sensitivity and every prevalence or true-share correction are therefore exploratory. Main results. The criterion flags almost all expert-unreadable windows but only subsets of the windows labelled inconclusive by their corpora: an inconclusive rate measures convention as much as signal. At tau_a = 0.50, criterion-positive windows are 8.1%, 56.7% and 69.6% of the strict proxy loss on Holter, handheld and patch. The true-share point intervals lie below 50% on handheld and above it on patch, but joint calibration-and-sampling envelopes span 50% on both and still do at the Youden threshold 0.60. On handheld, the calibration-only envelope is more than twice as wide as the corpus-sampling-only envelope. Gate-versus-confidence ordering changes with the accounting and corpus; no general selective-risk advantage of the gate is established. Significance. Corpus labels, criterion flags and latent expert unreadability must not be conflated. A gate can win by predicting a labelling convention, and this retrospective study validates neither transfer to smartwatch dry electrodes nor improvement in patient outcomes. Measure the inconclusive rate with a stated criterion on the raw signal; do not inherit it from a corpus label. CI - (c) 2026 Institute of Physics and Engineering in Medicine. All rights, including for text and data mining, AI training, and similar technologies, are reserved. FAU - Cheng, Yunfei AU - Cheng Y AUID- ORCID: 0000-0002-0634-5830 AD - Health Technology and Engineering Research Center, SKG Health Technologies Co., Ltd., 23A Floor, Building 3, Zhongke R&D Park, No.009, Gaoxin South 1st Road, High-tech Zone Community, Yuehai street, Nanshan District, Shenzhen, Guangdong, 518063, China. FAU - Deng, Peng AU - Deng P AUID- ORCID: 0000-0001-8980-9903 AD - Health Technology and Engineering Research Center, SKG Health Technologies Co., Ltd., 23A Floor, Building 3, Zhongke R&D Park, No.009, Gaoxin South 1st Road, High-tech Zone Community, Yuehai street, Nanshan District, Shenzhen, Guangdong, 518063, China. FAU - Lian, Yun AU - Lian Y AD - Health Technology and Engineering Research Center, SKG Health Technologies Co., Ltd., 23A Floor, Building 3, Zhongke R&D Park, No.009, Gaoxin South 1st Road, High-tech Zone Community, Yuehai street, Nanshan District, Shenzhen, Guangdong, 518063, China. FAU - Yang, Ruichen AU - Yang R AD - Health Technology and Engineering Research Center, SKG Health Technologies Co., Ltd., 23A Floor, Building 3, Zhongke R&D Park, No.009, Gaoxin South 1st Road, High-tech Zone Community, Yuehai street, Nanshan District, Shenzhen, Guangdong, 518063, China. FAU - Sun, Huibin AU - Sun H AD - Health Technology and Engineering Research Center, SKG Health Technologies Co., Ltd., 23A Floor, Building 3, Zhongke R&D Park, No.009, Gaoxin South 1st Road, High-tech Zone Community, Yuehai street, Nanshan District, Shenzhen, Guangdong, 518063, China. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Physiol Meas JT - Physiological measurement JID - 9306921 SB - IM OTO - NOTNLM OT - atrial fibrillation OT - inconclusive ECG OT - selective classification OT - signal quality assessment OT - single-lead ECG OT - wearable health monitoring EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:03 PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:03 [entrez] AID - 10.1088/1361-6579/aebae3 [doi] PST - aheadofprint SO - Physiol Meas. 2026 Oct 28. doi: 10.1088/1361-6579/aebae3. PMID- 42950423 OWN - NLM STAT- Publisher LR - 20261028 IS - 1361-648X (Electronic) IS - 0953-8984 (Linking) DP - 2026 Oct 28 TI - Softening of low-energy anharmonic phonon modes in rutile TiO(2)investigated by inelastic x-ray and neutron scattering. LID - 10.1088/1361-648X/aebad6 [doi] AB - Low-energy anharmonic phonons in rutile TiO(2)were investigated by inelastic x-ray and neutron scattering (IXS and INS) techniques. Owing to the different sensitivities of IXS and INS to phonon modes, the transverse optical branches corresponding to the B(1u)and A(2u)modes at the Gamma point were selectively observed in the respective spectra. It was found that the lowest-energy B(1u)mode exhibits pronounced softening with decreasing temperature, while the A(2u)mode also shows softening, as previously reported. The observed temperature-dependent softening was well reproduced by density functional theory calculations based on the stochastic self-consistent harmonic approximation, although the magnitude of the softening was quantitatively underestimated. The pronounced softening of the B(1u)mode, as well as its finite linewidth, is discussed in terms of possible higher-order phonon interactions between the B(1u)and A(2u)modes. The implications of this strong anharmonicity for lattice-related physicochemical properties, including dielectric response, thermal transport, Li-ion insertion, and polaron-related photocatalytic phenomena, are also addressed. CI - (c) 2026 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved. FAU - Moriguchi, Kota AU - Moriguchi K AD - Department of Physical Science and Engineering, Nagoya Institute of Technology, Gokisocho, Showaku, Nagoya, Aichi, 466-8555, Japan. FAU - Kimura, Koji AU - Kimura K AUID- ORCID: 0000-0001-5485-3672 AD - Department of Physical Science and Engineering, Nagoya Institute of Technology, Gokisocho, Showaku, Nagoya, 466-8555, Japan. FAU - Hashimoto, Yujiro AU - Hashimoto Y AUID- ORCID: 0009-0005-8060-7340 AD - Department of Physics, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan. FAU - Dung, Ngoc AU - Dung N AD - Department of Precision Engineering, Osaka University, 2-1 Yamadaoka, Suita, 565-0871, Japan. FAU - Tsutsui, Satoshi AU - Tsutsui S AD - Japan Synchrotron Radiation Research Institute, 1-1-1, Kouto, Sayo District, Hyogo Prefecture, 679-5198, Japan. FAU - Manjo, Taishun AU - Manjo T AD - Precision Spectroscopy Division, Japan Synchrotron Radiation Research Institute, 1-1-1, Kouto, Sayo District, Hyogo Prefecture, 679-5198, Japan. FAU - Fukui, Hiroshi AU - Fukui H AUID- ORCID: 0000-0002-7880-635X AD - Precision Spectroscopy Division, JASRI, Kouto 1-1-1, Sayo District, Hyogo, 679-5198, Japan. FAU - Ishikawa, Daisuke AU - Ishikawa D AD - Kokido Hikari Kagaku Kenkyu Center, 1-1-1, Kouto, Sayo-gun, Hyogo, 679-5198, Japan. FAU - Baron, Alfred Q R AU - Baron AQR AUID- ORCID: 0000-0002-5899-2116 AD - Materials Dynamics Laboratory, RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo, Hyogo, 679-5148, Japan. FAU - Ikeda, Yoichi AU - Ikeda Y AD - Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi Prefecture, 980-8577, Japan. FAU - Halim, Harry Handoko AU - Halim HH AUID- ORCID: 0000-0001-6285-1650 AD - Graduate School of Engineering, Osaka University, Osaka University, 2-1 Yamadaoka Suita Osaka JAPAN, Suita, 565-0871, Japan. FAU - Nakano, Akitoshi AU - Nakano A AD - Department of Applied Physics, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan. FAU - Morikawa, Yoshitada AU - Morikawa Y AUID- ORCID: 0000-0003-4895-4121 AD - Department of Precision Engineering, Osaka University, Graduate School of Engineering, Osaka University, Yamada-oka 2-1, Suita, 565-0871, Japan. FAU - Taniguchi, Hiroki AU - Taniguchi H AD - Department of Physics, Nagoya University, Furo-cho, Chikusa-ku,, Nagoya, 464-8601, Japan. FAU - Hayashi, Koichi AU - Hayashi K AD - Nagoya Institute of Technology, Gokisocho, Showaku, Nagoya, Aichi, 4668555, Japan. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - J Phys Condens Matter JT - Journal of physics. Condensed matter : an Institute of Physics journal JID - 101165248 SB - IM OTO - NOTNLM OT - anharmonic phonons OT - inelastic neutron scattering OT - inelastic x-ray scattering OT - lattice dynamics OT - rutile TiO2 EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:03 PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:03 [entrez] AID - 10.1088/1361-648X/aebad6 [doi] PST - aheadofprint SO - J Phys Condens Matter. 2026 Oct 28. doi: 10.1088/1361-648X/aebad6. PMID- 42950404 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-7072 (Electronic) IS - 0308-8146 (Linking) VI - 532 IP - Pt 4 DP - 2026 Oct 14 TI - Reassessing the link between soluble solids and sugars in melon: FTIR models as an alternative tool. PG - 151549 LID - S0308-8146(26)03709-X [pii] LID - 10.1016/j.foodchem.2026.151549 [doi] AB - Soluble solids content (SSC, degrees Brix) is widely used to estimate melon sugar content. However, in a diverse set of Ibericus melons, it correlated moderately (R(2)  approximately  0.60) with total sugars and sucrose, and not with hexoses content or the hexose-to-sucrose ratio. Moreover, the sensitivity of SSC under high selection pressure for high sugar (10%-20%) remained low (55%-60%). In contrast, FTIR trial specific models predicted sucrose (s), hexoses (h), citric acid (not malic), total sugars, sucrose equivalents, and the h to s ratio with mean prediction R(2) ranging 0.73-0.88 and errors ranging 3.5-8.9%. A general model, performed similarly to the weakest specific one, strongly affected by low fructose levels, resulting in mean prediction R(2) of 0.63 (external validation). Externally validated specific models achieved prediction R(2) values of 0.79-0.94 (mean 0.84) and errors ranging 3.6-28.7% (mean 11%). FTIR represents a good alternative to SSC for high-throughput phenotyping. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved. FAU - Rosello, Salvador AU - Rosello S AD - Joint Research Unit UJI/UPV - Improvement of Agri-Food Quality, Department de Ciencies Agraries i del Medi Natural, Universitat Jaume I, Avda. Sos Baynat s/n, Castellon, 12071, Spain. FAU - de Castro, Ana Perez AU - de Castro AP AD - Joint Research Unit UJI/UPV - Improvement of Agri-Food Quality, COMAV, Universitat Politecnica de Valencia, Cno. de Vera, s.n, Valencia, 46022, Spain. FAU - Pico, Belen AU - Pico B AD - COMAV, Instituto de Conservacion y Mejora de la Agrodiversidad, Universitat Politecnica de Valencia, Cno. de Vera, s.n, Valencia, 46022, Spain. FAU - Garcia-Cabrera, Maria AU - Garcia-Cabrera M AD - COMAV, Instituto de Conservacion y Mejora de la Agrodiversidad, Universitat Politecnica de Valencia, Cno. de Vera, s.n, Valencia, 46022, Spain. FAU - Cebolla-Cornejo, Jaime AU - Cebolla-Cornejo J AD - Joint Research Unit UJI/UPV - Improvement of Agri-Food Quality, COMAV, Universitat Politecnica de Valencia, Cno. de Vera, s.n, Valencia, 46022, Spain. Electronic address: jaicecor@upv.es. LA - eng PT - Journal Article DEP - 20261014 PL - England TA - Food Chem JT - Food chemistry JID - 7702639 SB - IM OTO - NOTNLM OT - Breeding OT - Brix OT - Cucumis melo L OT - FT-MIR OT - Fourier-transform mid-infrared spectroscopy OT - Selection OT - Soluble solids content OT - Spectroscopy COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Belen Pico reports financial support was provided by Generalitat Valenciana. Belen Pico reports financial support was provided by Agencia Estatal de Investigacion. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:02 PHST- 2026/03/26 00:00 [received] PHST- 2026/09/11 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:02 [entrez] AID - S0308-8146(26)03709-X [pii] AID - 10.1016/j.foodchem.2026.151549 [doi] PST - aheadofprint SO - Food Chem. 2026 Oct 14;532(Pt 4):151549. doi: 10.1016/j.foodchem.2026.151549. PMID- 42950397 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-033X (Electronic) IS - 0959-440X (Linking) VI - 102 DP - 2026 Oct 28 TI - Modulation of pre-mRNA splicing by small molecules. PG - 103423 LID - S0959-440X(26)00205-8 [pii] LID - 10.1016/j.sbi.2026.103423 [doi] AB - Alternative splicing of mRNA precursors is an important mechanism of gene regulation, which is frequently altered in a variety of pathologies. Recent advances demonstrate that modulating splice-site selection can offer new therapeutic options for unmet medical needs. Here, we review structural and functional data that help explain basic principles of splice site selection and their modulation by small molecules. These include selective effects of inhibitors of branch point recognition, as well as the effects of intercalating compounds that serve to repair imperfect base-pairing interactions important for 5' splice site recognition. The remarkable complexity and versatility of the spliceosome, which removes introns with high precision despite the diversity of splice-site sequences, can offer unique opportunities to selectively modulate splice-site selection for gene regulation and therapeutic applications. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved. FAU - Arecco, Niccolo AU - Arecco N AD - Genome Biology Program, Centre for Genomic Regulation (CRG), The Barcelona Institute for Science and Technology, Barcelona, Spain. Electronic address: arecco.niccolo@gmail.com. FAU - Valcarcel, Juan AU - Valcarcel J AD - Genome Biology Program, Centre for Genomic Regulation (CRG), The Barcelona Institute for Science and Technology, Barcelona, Spain; Universitat Pompeu Fabra (UPF), Barcelona, Spain; Institucio Catalana de Recerca i Estudis Avancats (ICREA), Barcelona, Spain. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - Curr Opin Struct Biol JT - Current opinion in structural biology JID - 9107784 SB - IM COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Juan Valcarcel Juarez reports financial support was provided by Center for Genomic Regulation. Juan Valcarcel reports a relationship with Center for Genomic Regulation that includes employment. Juan Valcarcel is a member of the scientific advisory board of Remix Therapeutics and Stoke Therapeutics, and a founder of Alterna Therapeutics. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:02 PHST- 2026/06/26 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:02 [entrez] AID - S0959-440X(26)00205-8 [pii] AID - 10.1016/j.sbi.2026.103423 [doi] PST - aheadofprint SO - Curr Opin Struct Biol. 2026 Oct 28;102:103423. doi: 10.1016/j.sbi.2026.103423. PMID- 42950396 OWN - NLM STAT- Publisher LR - 20261028 IS - 1878-7479 (Electronic) IS - 1878-7479 (Linking) VI - 24 IP - 1 DP - 2026 Oct 28 TI - HDAC3 inhibition alleviates neuroinflammation and white matter injury after subarachnoid hemorrhage through modulation of BDNF/TrkB/NF-kappaB signaling. PG - e01099 LID - S1878-7479(26)00269-2 [pii] LID - 10.1016/j.neurot.2026.e01099 [doi] AB - White matter injury (WMI) is a major contributor to neurological deficits after subarachnoid hemorrhage (SAH). Histone deacetylase 3 (HDAC3) is an established modulator of inflammatory responses, yet its contribution to post-SAH white matter pathology remains poorly understood. We used a rat endovascular perforation model and treated animals with RGFP966, a selective HDAC3 inhibitor. Clinical data, single-cell RNA sequencing (scRNA-seq), magnetic resonance imaging (MRI), and transmission electron microscopy were integrated to evaluate cellular remodeling and structural integrity. Short- and long-term neurological recovery was assessed using a systematic battery of behavioral tests, while the underlying BDNF/TrkB/NF-kappaB signaling axis was validated through gain- and loss-of-function experiments. HDAC3 levels were elevated in both the CSF of SAH patients and rat brain tissues, inversely correlating with myelin basic protein (MBP) expression. RGFP966 treatment effectively preserved WMI, evidenced by reduced axonal injury and improved myelin shielding, and translated to superior long-term cognitive performance. ScRNA-seq analysis identified a profound shift in the microglial and oligodendrocyte-lineage landscapes after SAH. HDAC3 inhibition effectively curbed the expansion of pro-inflammatory microglial subsets while restoring homeostatic signatures and enhancing pro-myelination programs. Mechanistically, RGFP966 upregulated BDNF expression and restored TrkB phosphorylation, thereby suppressing NF-kappaB-mediated neuroinflammation. Notably, BDNF knockdown abolished the protective effects of HDAC3 inhibition, whereas exogenous BDNF mimicked the anti-inflammatory and myelin-protective phenotype induced by RGFP966. In conclusion, our findings demonstrate that HDAC3 is a pivotal driver of post-SAH neuroinflammation and WMI. Inhibition of HDAC3 exerts neuroprotection by recalibrating the microglial immune microenvironment and restoring the BDNF/TrkB axis. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Inc. All rights reserved. FAU - Yang, Hongqiao AU - Yang H AD - Ningbo Key Laboratory of Nervous System and Brain Function, Department of Neurosurgery, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, 315010, China. FAU - Hu, Ziliang AU - Hu Z AD - Ningbo Key Laboratory of Nervous System and Brain Function, Department of Neurosurgery, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, 315010, China. FAU - Zhuang, Hao AU - Zhuang H AD - Ningbo Key Laboratory of Nervous System and Brain Function, Department of Neurosurgery, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, 315010, China. FAU - Chen, Siqi AU - Chen S AD - Ningbo Key Laboratory of Nervous System and Brain Function, Department of Neurosurgery, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, 315010, China. FAU - Zhou, Shengjun AU - Zhou S AD - Ningbo Key Laboratory of Nervous System and Brain Function, Department of Neurosurgery, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, 315010, China. FAU - Sun, Meng AU - Sun M AD - Ningbo Key Laboratory of Nervous System and Brain Function, Department of Neurosurgery, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, 315010, China. FAU - Lian, Lifei AU - Lian L AD - Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. FAU - Zhou, Chenhui AU - Zhou C AD - Ningbo Key Laboratory of Nervous System and Brain Function, Department of Neurosurgery, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, 315010, China. FAU - Gao, Xiang AU - Gao X AD - Ningbo Key Laboratory of Nervous System and Brain Function, Department of Neurosurgery, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, 315010, China. Electronic address: fyygaoxiang@nbu.edu.cn. FAU - Huang, Yi AU - Huang Y AD - Ningbo Key Laboratory of Nervous System and Brain Function, Department of Neurosurgery, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, 315010, China. Electronic address: huangy102@gmail.com. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Neurotherapeutics JT - Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics JID - 101290381 SB - IM OTO - NOTNLM OT - HDAC3 OT - Neuroinflammation OT - Single-cell RNA sequencing OT - Subarachnoid hemorrhage OT - White matter injury COIS- Competing interests The authors declare no conflict of interest. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:02 PHST- 2026/05/12 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:02 [entrez] AID - S1878-7479(26)00269-2 [pii] AID - 10.1016/j.neurot.2026.e01099 [doi] PST - aheadofprint SO - Neurotherapeutics. 2026 Oct 28;24(1):e01099. doi: 10.1016/j.neurot.2026.e01099. PMID- 42950387 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-4367 (Electronic) IS - 0927-7765 (Linking) VI - 270 DP - 2026 Oct 23 TI - Biomimetic PLGA nanoparticles-mediated low-intensity pulsed ultrasound-triggered targeted release of C646 for esophageal cancer therapy. PG - 116281 LID - S0927-7765(26)00869-6 [pii] LID - 10.1016/j.colsurfb.2026.116281 [doi] AB - Esophageal squamous cell carcinoma (ESCC) is associated with high morbidity and poor clinical prognosis among the Chinese population. Conventional chemoradiotherapy is hampered by severe systemic toxic effects and inadequate tumor-targeting performance. EP300 represents a promising epigenetic therapeutic target for ESCC. Nevertheless, the small-molecule EP300 inhibitor C646 suffers from low target selectivity and substantial off-target toxicity. Herein, we constructed homotypic tumor-cell-membrane-coated, C646-loaded PLGA nanoparticles (C646/PLGA@CM NPs). This nanosystem integrates biomimetic homologous targeting, immune evasion capability, and low intensity pulsed ultrasound (LIPUS, US)-triggered on-demand release for combinatorial therapy against ESCC. Physicochemical characterizations confirmed successful membrane coating, satisfactory colloidal stability, and US-triggered drug-release profiles. Cellular assays demonstrated that C646/PLGA@CM NPs achieve efficient homologous uptake in tumor cells and evade phagocytosis by macrophages. Quantitative analysis using SynergyFinder confirmed strong synergistic antitumor effects of the nanoparticles combined with US. In vitro assays showed that C646/PLGA@CM combined with US markedly suppressed the proliferation and migration of KYSE-140 cells by inducing G2/M-phase cell-cycle arrest and reversing epithelial-mesenchymal transition (EMT). Furthermore, results from ESCC subcutaneous xenograft mouse models revealed that C646/PLGA@CM combined with US exerted potent in-vivo antitumor efficacy with satisfactory biosafety. Transcriptomic profiling together with qRT-PCR assays indicated that the antitumor mechanism involves EP300-inhibition-mediated activation of the p53/FoxO pathway, alongside dysregulation of tumor-suppressive and oncogenic miRNAs. Collectively, this CM-coated nanoplatform combined with US offers a feasible precision-targeted therapeutic strategy for ESCC. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Xu, Jing AU - Xu J AD - Department of Ultrasonography, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, China; Department of Ultrasonography, Shandong University affiliation Shandong Provincial Third Hospital, Ji'nan, China. FAU - Pang, Shudong AU - Pang S AD - Department of hepatobiliary Surgery, Shandong University affiliation Shandong Provincial Third Hospital, Ji'nan, China. FAU - Kang, Yasheng AU - Kang Y AD - Department of Ultrasound, Peoples Hospital of Inner Mongolia Autonomous Region, Hohhot, China. FAU - Wu, Liping AU - Wu L AD - Department of Ultrasonography, Shandong University affiliation Shandong Provincial Third Hospital, Ji'nan, China. FAU - Ma, Haijun AU - Ma H AD - Department of Ultrasonography, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, China; Department of Ultrasound, Tai'an Maternity and Child Health Care Hospital, Tai'an, China. FAU - Lyu, Yanling AU - Lyu Y AD - Department of Ultrasonography, Shandong University affiliation Shandong Provincial Third Hospital, Ji'nan, China. Electronic address: 380929402@qq.com. FAU - Li, Shilin AU - Li S AD - Department of Ultrasonography, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, China. Electronic address: lslqz@fjmu.edu.cn. LA - eng PT - Journal Article DEP - 20261023 PL - Netherlands TA - Colloids Surf B Biointerfaces JT - Colloids and surfaces. B, Biointerfaces JID - 9315133 SB - IM OTO - NOTNLM OT - Cancer cell membrane-coated nanoparticles OT - EP300 OT - Esophageal squamous cell carcinoma OT - Low intensity pulsed ultrasound OT - Nanomaterials COIS- Declaration of Competing Interest The authors declare no conflict of interest. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:02 PHST- 2026/07/10 00:00 [received] PHST- 2026/10/20 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:02 [entrez] AID - S0927-7765(26)00869-6 [pii] AID - 10.1016/j.colsurfb.2026.116281 [doi] PST - aheadofprint SO - Colloids Surf B Biointerfaces. 2026 Oct 23;270:116281. doi: 10.1016/j.colsurfb.2026.116281. PMID- 42950386 OWN - NLM STAT- Publisher LR - 20261028 IS - 1532-3080 (Electronic) IS - 0960-9776 (Linking) VI - 90 DP - 2026 Oct 12 TI - To stop or not to stop? International physician survey on HER2 targeted therapy for metastatic breast cancer patients in remission. PG - 104935 LID - S0960-9776(26)00245-6 [pii] LID - 10.1016/j.breast.2026.104935 [doi] AB - INTRODUCTION: HER2-targeted therapy (HER2-TT) has improved outcomes in HER2+ metastatic breast cancer (MBC) with increasing numbers of long-term survivors in complete response (CR). Little is known about discontinuing maintenance HER2-TT in these patients. METHODS: An online survey of physicians was conducted via the Breast International Group (BIG) network between 30 October 2024 and 1 January 2025 to gather insights on HER2-TT use. RESULTS: A total of 237 physicians from 197 institutions in 38 countries answered the questionnaire. Most reported full access to trastuzumab (88.6%) and pertuzumab (80.6%). While 23.6% of physicians estimated that >/=30% of their HER2+ MBC patients diagnosed over the past decade are still alive >5 years post diagnosis, 86.1% reported that <25% of patients with no evidence of disease after treatment have discontinued HER2-TT. About 59.5% believed that HER2+ MBC can be cured, while 21.9% were unsure, and 18.6% believed that it cannot be cured. Less than half (43.9%) felt that maintenance HER2-TT could be stopped in patients in CR for prolonged period; 49.8% selected "maybe". Opinions on timing of discontinuation after achieving CR were split: 21.1% favoured stopping after 1-<3 years, 25.3% after 3-<5 years and 40.5% after >/=5 years. Patient's choice was the most important factor in the decision making for discontinuing HER2-TT for 43.0% of physicians, followed by safety (31.6%) and physician's recommendation (15.2%). CONCLUSIONS: Although majority of physicians believed that HER2+ MBC can be cured, most do not discontinue maintenance HER2-TT. Further data on outcomes after treatment cessation are needed to inform evidence-based optimization strategies. CI - Copyright (c) 2026 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Lee, Han Yi AU - Lee HY AD - Division of Medical Oncology, National Cancer Centre Singapore, Singapore, Singapore. FAU - Ong, Whee Sze AU - Ong WS AD - Division of Clinical Trials and Epidemiology Sciences, National Cancer Centre Singapore, Singapore, Singapore. FAU - Tjan-Heijnen, Vivianne AU - Tjan-Heijnen V AD - Department of Medical Oncology, Maastricht University Medical Center+, Maastricht, the Netherlands. FAU - Lambertini, Matteo AU - Lambertini M AD - Department of Internal Medicine and Medical Specialties (Di.M.I.), School of Medicine, University of Genova, Genova, Italy; Medical Oncology Unit, Ente Ospedaliero Ospedali Galliera, Genova, Italy. FAU - Lu, Yen Shen AU - Lu YS AD - Department of Oncology, National Taiwan University Hospital, Taipei, Taiwan. FAU - Park, Kyong Hwa AU - Park KH AD - Division of Hematology-Oncology, Department of Internal Medicine, University Anam Hospital, Republic of Korea; University College of Medicine, Seoul, Republic of Korea. FAU - Sagara, Yasuaki AU - Sagara Y AD - Department of Breast and Thyroid Surgical Oncology, Sagara Hospital, Hakuaikai Medical Corporation, Kagoshima, Japan. FAU - Vidal, Maria AU - Vidal M AD - Hospital Clinic de Barcelona, Barcelona, Spain. FAU - Urruticoechea, Ander AU - Urruticoechea A AD - Medical Oncology dep. Donostia University Hospital - Biogipuzkoa. Osakidetza GEICAM (Spanish Breast Cancer Group), Spain. FAU - Oliveira, Mafalda AU - Oliveira M AD - Medical Oncology Department, Vall d'Hebron University Hospital and Breast Cancer Group, Vall d'Hebron Institute of Oncology (VHIO), Barcelona, Spain. FAU - Ciruelos, Eva AU - Ciruelos E AD - Instituto de Investigacion Sanitaria Hospital 12 de Octubre (imas12), Madrid, Spain. FAU - Zdenkowski, Nicholas AU - Zdenkowski N AD - School of Medicine and Public Health, University of Newcastle, NSW, Australia; Breast Cancer Trials, Newcastle, NSW, Australia. FAU - Bedard, Philippe L AU - Bedard PL AD - Division of Medical Oncology and Hematology, Princess Margaret Cancer Centre, University Health Network, Toronto, ON, Canada; Department of Medicine, University of Toronto, Toronto, ON, Canada. FAU - Ignatiadis, Michail AU - Ignatiadis M AD - Department of Medical Oncology, Institut Jules Bordet, Hopital Universitaire de Bruxelles, Universite Libre de Bruxelles, Brussels, Belgium. FAU - Lee, Soo Chin AU - Lee SC AD - Department of Haematology-Oncology, National University Cancer Institute, Singapore, Singapore. FAU - Sohn, Joohyuk AU - Sohn J AD - Division of Medical Oncology, Yonsei University College of Medicine, Yonsei Cancer Center, Seoul, Republic of Korea. FAU - Zaman, Khalil AU - Zaman K AD - Department of Medical Oncology, Lausanne University Hospital, CHUV and University of Lausanne, Switzerland. FAU - Werutsky, Gustavo AU - Werutsky G AD - Latin American Cooperative Oncology Group (LACOG), Porto Alegre, Brazil. FAU - Curigliano, Giuseppe AU - Curigliano G AD - Division of Early Drug Development for Innovative Therapies, European Institute of Oncology IRCCS, Milano, Italy; Department of Oncology and Hemato-Oncology, University of Milano, Milan, Italy. FAU - O'Reilly, Seamus AU - O'Reilly S AD - Cancer Trials Ireland, 123 St Stephens Green, Dublin, Ireland. FAU - Tsang, Janice AU - Tsang J AD - School of Clinical Medicine, The University of Hong Kong, Hong Kong Special Administrative Region of China. FAU - Kim, Sung-Bae AU - Kim SB AD - Department of Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea. FAU - Saji, Shigehira AU - Saji S AD - Department of Medical Oncology, Fukushima Medical University, Fukushima, Japan. FAU - Ohno, Shinji AU - Ohno S AD - Department of Breast and Thyroid Surgical Oncology, Sagara Hospital, Hakuaikai Medical Corporation, Kagoshima, Japan. FAU - Adam, Virginie AU - Adam V AD - Breast International Group (BIG), Brussels, Belgium. FAU - Arahmani, Amal AU - Arahmani A AD - Breast International Group (BIG), Brussels, Belgium. FAU - Caballero, Carmela AU - Caballero C AD - Breast International Group (BIG), Brussels, Belgium. FAU - Yap, Yoon Sim AU - Yap YS AD - Division of Medical Oncology, National Cancer Centre Singapore, Singapore, Singapore; Duke-NUS (National University of Singapore) Medical School, Singapore. Electronic address: gmsyapys@duke-nus.edu.sg. LA - eng PT - Journal Article DEP - 20261012 PL - Netherlands TA - Breast JT - Breast (Edinburgh, Scotland) JID - 9213011 SB - IM COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Han Yi Lee reports relationships with Novartis and Roche that include speaking and lecture fees; and with Gilead, Daiichi Sankyo, AstraZeneca, and Novartis that include travel reimbursement. Whee Sze Ong declares no competing interests. Vivianne Tjan-Heijnen reports relationships with Novartis and Eli Lilly that include speaking and lecture fees. Matteo Lambertini reports relationships with Roche, Eli Lilly, Novartis, AstraZeneca, Pfizer, Gilead, MSD, Pierre Fabre, Menarini, Nordic Pharma, Bayer, Ipsen, and Daiichi Sankyo that include consultation or advisory fees; with Roche, Eli Lilly, Novartis, Pfizer, AstraZeneca, Takeda, Ipsen, Sandoz, Libbs, Daiichi Sankyo, Gilead, and Menarini that include speaking and lecture fees; with Gilead, Daiichi Sankyo, and Roche that include travel reimbursement; and with Gilead that include institutional research support. Yen Shen Lu reports grant support from/research collaborations with Novartis, Pfizer, MSD, Roche, Astra Zeneca, ACT Genomics; advisory board/speaker invitation from Novartis, Pfizer, MSD, Roche, Astra Zeneca, Eisai, Eli Lilly, Daiichi Sankyo and Europharma; conference support from Novartis and Eisai. Kyong Hwa Park reports relationships with AstraZeneca, Gilead, Roche, Pfizer, Celtrion, Daewoong and Daiichi Sankyo that include consultation or advisory fees; with iMBDx that include equity and stock ownership; and with Celltrion that include speaking and lecture fees. Yasuaki Sagara reports relationships with Nippon Boehringer Ingelheim, Eli Lilly Japan, Chugai Pharma, AstraZeneca Japan, MSD K.K., Novartis, Nippon Kayaku, Kyowa Kirin International, and Daiichi Sankyo/UCB Japan that include consultation or advisory fees; and with AstraZeneca, Chugai Pharma, Eisai, Pfizer, Eli Lilly, MSD, and Daiichi Sankyo Japan that include speaking and lecture fees. Maria Vidal reports relationships with AstraZeneca, Adamed, Daiichi Sankyo, Pfizer, and Roche that include consultation or advisory fees; with AstraZeneca, Daiichi Sankyo, Gilead Sciences, Guardant Health, Novartis, Pfizer, Roche/Genentech, and Veracyte that include speaking and lecture fees; and with Gilead Sciences, Roche, Pfizer, Novartis, AstraZeneca, and Daiichi Sankyo that include travel reimbursement. Ander Urruticoechea reports relationships with Ellipses Pharma, Eisai, Menarini Stemline, and Oncomatryx that include consultation or advisory fees; with Gilead, Menarini Stemline, and Eli Lilly that include speaking and lecture fees; and with Gilead, Novartis, AstraZeneca, Daiichi Sankyo, and Roche that include travel reimbursement. Mafalda Oliveira reports board memberships with SOLTI Cancer Research Group (President, 2023-2027); relationships with AstraZeneca, Daiichi Sankyo, Gilead, Hoffmann-La Roche, Eli Lilly, MSD, Novartis, Pfizer, and ProteinQure that include consultation or advisory fees; with AstraZeneca, Curio Science, Gilead, i-One, Eli Lilly, and MSD that include speaking and lecture fees; and with AstraZeneca, Gilead, Pfizer, and Roche that include travel reimbursement. Eva Cieruelos reports board memberships with Instituto de Salud Carlos III (Scientific Evaluator) and SOLTI Cooperative Group (Member of the Board of Directors); relationships with AstraZeneca, Avenzo, BeiGene, Daiichi Sankyo, Eli Lilly, MSD, Novartis, Pfizer, Reveal Genomics, and Roche that include consultation or advisory fees; with Gilead, Eli Lilly, Pfizer, and Roche that include speaking and lecture fees; and with AstraZeneca that include travel reimbursement. Nicholas Zdenkowski reports relationships with Eli Lilly, Eisai, AstraZeneca, MSD, Novartis, Gilead, Roche, Menarini, and Pfizer that include consultation or advisory fees; and with Roche, Pfizer, Eisai, Amgen, Gilead, Novartis, Eli Lilly, AstraZeneca, and MSD that include speaking and lecture fees. Philippe L. Bedard reports a board membership with the Myeloma Cancer Research Network; relationships with Bayer, Daiichi Sankyo/UCB Japan, and Eli Lilly that include consultation or advisory fees; with Boehringer Ingelheim that include speaking and lecture fees; and other relationships with Relay Therapeutics and Eli Lilly. Michail Ignatiadis reports relationships with Pfizer, Daiichi Sankyo, AstraZeneca, Menarini Stemline, Gilead Sciences, and Novartis that include consultation or advisory fees; and with Gilead, Roche, and AstraZeneca that include travel reimbursement. Soo Chin Lee reports relationships with Pfizer, Novartis, AstraZeneca, Eli Lilly, MSD, Roche, Gilead, DKSH, Daiichi Sankyo, and Menarini that include consultation or advisory fees and speaking and lecture fees. Joohyuk Sohn reports relationships with Seagen, MSD, Roche, Pfizer, Novartis, AstraZeneca, Eli Lilly, GSK, Boehringer Ingelheim, Sanofi, Daiichi Sankyo, Qurient, Dragonfly, Eikon, Gilead, Celcuity, Bristol Myers Squibb, HLB Life Science, Sermonix Pharmaceuticals, Olema, Hanmi Pharm, Ildong Pharmaceutical, and Samyang Holdings that include funding or grants. Khalil Zaman reports relationships with Roche, Daiichi Sankyo, AstraZeneca, Gilead Sciences, Eli Lilly, Novartis, and Pfizer that include consultation or advisory fees and speaking and lecture fees; with Agendia, AstraZeneca, MSD, Daiichi Sankyo, Exact Sciences, Eli Lilly, Pierre Fabre, Gilead Sciences, Novartis, Pfizer, and Roche that include funding or grants; and with Pfizer, Astra Zeneca/MedImmune, Daiichi Sankyo, Merck/MSD that include speaking and lecture fees and travel reimbursement. Gustavo Werutsky reports relationships with AstraZeneca, Novartis, Daiichi Sankyo, and Roche that include consultation or advisory fees; with Novartis, Roche, Takeda Pharmaceutical, Janssen, and Libbs that include funding or grants; and with AstraZeneca, Merck & Co., Novartis, Pfizer, Roche, and Daiichi Sankyo that include speaking and lecture fees. Giuseppe Curigliano reports board memberships with ESMO (President-Elect), Europa Donna (Scientific Council), EUSOMA (Leadership Role until the end of 2026), and Fondazione Beretta; relationships with AstraZeneca, Bristol Myers Squibb, Daiichi Sankyo, Exact Sciences, Gilead, Eli Lilly, Menarini, Merck, Pfizer, Roche, and Veracyte that include consultation or advisory fees; and with AstraZeneca, Daiichi Sankyo, Novartis, Pfizer, and Roche that include speaking and lecture fees. Seamus O'Reilly reports relationships with AstraZeneca, Menarini, and Novartis that include consultation or advisory fees; and with Pfizer, Novartis, Daiichi Sankyo, and Merck that include travel reimbursement. Janice Tsang reports relationships with NanoString and Pfizer that include consultation or advisory fees; and with Amgen, NanoString, Novartis, Pfizer, and Roche that include speaking and lecture fees. Sung-Bae Kim reports relationships with Novartis, Eli Lilly, ISU Abix, AstraZeneca, Dae Hwa Pharmaceutical Co. Ltd., OBI Pharma, BeiGene, and Daiichi Sankyo that include consultation or advisory fees; with Novartis, DongKook Pharm Co., and Sanofi that include funding or grants; and with Novartis, Eli Lilly, Daiichi Sankyo, AstraZeneca, and OBI Pharma that include speaking and lecture fees. Shigehira Saji reports relationships with Chugai/Roche, AstraZeneca, Eli Lilly, Pfizer, Ono, Daiichi Sankyo, and MSD that include consultation or advisory fees; and with Chugai, Kyowa Kirin, MSD, Guardant Health, Eisai, Takeda, Daiichi Sankyo, Eli Lilly, AstraZeneca, Pfizer, Taiho, Ono, Myriad, Gilead, Exact Sciences, and Nipro that include speaking and lecture fees. Shinji Ohno reports relationships with Kyowa Kirin Co., Ltd., Eli Lilly Japan K.K., Eisai Co., Ltd., MSD K.K., and Gilead Sciences, Inc. That include speaking and lecture fees. Virginie Adam declares no competing interests. Amal Arahmani declares no competing interests. Carmela Caballero declares no competing interests. Yoon-Sim Yap reports relationships with AstraZeneca, Daiichi Sankyo, DKSH, Novartis, Roche, Pfizer, Olema, Menarini, PUMA, and Gilead that include consultation or advisory fees and speaking and lecture fees; with MSD that include funding or grants; and with AstraZeneca, Daiichi Sankyo, DKSH, Novartis, and Roche that include travel reimbursement. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:02 PHST- 2026/08/10 00:00 [received] PHST- 2026/09/02 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:02 [entrez] AID - S0960-9776(26)00245-6 [pii] AID - 10.1016/j.breast.2026.104935 [doi] PST - aheadofprint SO - Breast. 2026 Oct 12;90:104935. doi: 10.1016/j.breast.2026.104935. PMID- 42950383 OWN - NLM STAT- Publisher LR - 20261028 IS - 1090-2414 (Electronic) IS - 0147-6513 (Linking) VI - 326 DP - 2026 Oct 28 TI - Differential regulation of conjugative transfer and OMV-mediated ARG delivery by iprovalicarb and cycluron: Implications for pathway-specific risk assessment. PG - 120972 LID - S0147-6513(26)01302-3 [pii] LID - 10.1016/j.ecoenv.2026.120972 [doi] AB - Horizontal gene transfer (HGT) is a major driver of the rapid dissemination of antibiotic resistance genes (ARGs) among bacteria. Non-antibiotic compounds can promote plasmid-mediated conjugative transfer. In waterfowl farming, waterfowl frequently move among water, soil, and feces, which serve as reservoirs for pesticides and ARGs and thus potential hotspots for ARG spread. However, whether outer membrane vesicles (OMVs), an emerging ARG transmission pathway, are regulated by pesticides and respond in a manner consistent with conjugation is unclear. In this study, two pesticides detected in waterfowl farm environments, iprovalicarb and cycluron were selected to investigate their effects on conjugative transfer and OMV-mediated ARG dissemination. Both pesticides significantly promoted RP4 plasmid conjugation frequency at sub-inhibitory concentrations. This effect was associated with elevated bacterial reactive oxygen species (ROS), increased membrane permeability and fluidity, enhanced interbacterial contact, and altered energy metabolism. However, their effects on the OMV pathway diverged: iprovalicarb increased OMV production and vesicle size and was associated with more bla-positive colonies after OMV exposure, whereas cycluron inhibited OMV production. This decoupling suggests that pesticides can differentially regulate conjugative and OMV-mediated ARG transfer. However, the bla gene was not stably inherited in recipient bacteria. Overall, these findings highlight the need to evaluate these two pathways separately when assessing the risk of antimicrobial resistance. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Zhang, Shaqiu AU - Zhang S AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Chengdu, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China. FAU - Fan, Yan AU - Fan Y AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. FAU - He, Peijie AU - He P AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. FAU - Li, Qianlong AU - Li Q AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China. FAU - Wang, Mingshu AU - Wang M AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Chengdu, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China. FAU - Jia, Renyong AU - Jia R AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Chengdu, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China. FAU - Chen, Shun AU - Chen S AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Chengdu, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China. FAU - Liu, Mafeng AU - Liu M AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Chengdu, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China. FAU - Zhu, Dekang AU - Zhu D AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Chengdu, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China. FAU - Zhao, Xinxin AU - Zhao X AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Chengdu, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China. FAU - Wu, Ying AU - Wu Y AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Chengdu, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China. FAU - Yang, Qiao AU - Yang Q AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Chengdu, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China. FAU - Huang, Juan AU - Huang J AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Chengdu, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China. FAU - Ou, Xumin AU - Ou X AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Chengdu, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China. FAU - Sun, Di AU - Sun D AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Chengdu, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China. FAU - Tian, Bin AU - Tian B AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Chengdu, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China. FAU - He, Yu AU - He Y AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Chengdu, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China. FAU - Wu, Zhen AU - Wu Z AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Chengdu, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China. FAU - Cheng, Anchun AU - Cheng A AD - Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China; Institute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Chengdu, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, 611130, China; Institute of Veterinary Immunology and Green Drugs, Veterinary Department in College of Animal Science, State Key Laboratory of Green Pesticide, Guizhou University, Guiyang, 550025, China. Electronic address: chenganchun@vip.163.com. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Ecotoxicol Environ Saf JT - Ecotoxicology and environmental safety JID - 7805381 SB - IM OTO - NOTNLM OT - Antibiotic resistance genes OT - Conjugative transfer OT - Outer membrane vesicles OT - Pesticides COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:01 PHST- 2026/06/04 00:00 [received] PHST- 2026/09/04 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:01 [entrez] AID - S0147-6513(26)01302-3 [pii] AID - 10.1016/j.ecoenv.2026.120972 [doi] PST - aheadofprint SO - Ecotoxicol Environ Saf. 2026 Oct 28;326:120972. doi: 10.1016/j.ecoenv.2026.120972. PMID- 42950382 OWN - NLM STAT- Publisher LR - 20261028 IS - 1090-2414 (Electronic) IS - 0147-6513 (Linking) VI - 326 DP - 2026 Oct 28 TI - Analytical instability and aqueous degradation of carbapenem antibiotics: formation of beta-lactam-retaining transformation products. PG - 120986 LID - S0147-6513(26)01316-3 [pii] LID - 10.1016/j.ecoenv.2026.120986 [doi] AB - Carbapenems are last-resort antibiotics used to treat severe infections caused by multidrug-resistant bacteria. Despite their clinical importance and documented consumption, they remain largely undetected in environmental monitoring studies. This study investigated whether the lack of detection of carbapenems in environmental studies reflects analytical limitations, rapid degradation, or both. Sensitive LC-MS/MS methods were developed for selected carbapenems and used to assess compound stability under analytical and environmentally relevant conditions. All investigated carbapenems exhibited limited stability, with degradation strongly accelerated by methanol. Under environmentally relevant conditions, parent compounds degraded rapidly in river water, biofilm-containing water, and UV-exposed water, with little difference between treatments. High-resolution LC-MS/MS analysis revealed formation of multiple transformation products (TPs) retaining an intact beta-lactam ring. Meropenem generated four beta-lactam-retaining TPs, and faropenem generated four, several of which remained detectable long after disappearance of the parent compounds. These findings help explain the lack of detection of carbapenems in environmental surveys and demonstrate that disappearance of parent compounds does not necessarily imply elimination of beta-lactam-containing structures. The persistence of beta-lactam-retaining TPs suggests that environmental exposure and associated selective pressure may persist after parent carbapenems have degraded, highlighting the need for further investigation of their biological activity and potential role in antimicrobial resistance. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Martinez I Quer, Alba AU - Martinez I Quer A AD - Department of Environmental Science, Aarhus University, Frederiksborgvej 399, Roskilde, 4000, Denmark. FAU - Toussaint, Antonin AU - Toussaint A AD - Department of Environmental Science, Aarhus University, Frederiksborgvej 399, Roskilde, 4000, Denmark; Ecole Nationale Superieure de Chimie de Rennes, 11 Allee de Beaulieu, CS 50837, Cedex 7, Rennes, 35708, France. FAU - Jessen, Else AU - Jessen E AD - Department of Environmental Science, Aarhus University, Frederiksborgvej 399, Roskilde, 4000, Denmark; Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, Frederiksberg C, 1871, Denmark. FAU - Kisielius, Vaidotas AU - Kisielius V AD - Department of Environmental Science, Aarhus University, Frederiksborgvej 399, Roskilde, 4000, Denmark. Electronic address: vk@envs.au.dk. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Ecotoxicol Environ Saf JT - Ecotoxicology and environmental safety JID - 7805381 SB - IM OTO - NOTNLM OT - Degradation kinetics OT - Emerging contaminants OT - High-resolution mass spectrometry OT - Micropollutants OT - Suspect screening COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:01 PHST- 2026/07/01 00:00 [received] PHST- 2026/10/23 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:01 [entrez] AID - S0147-6513(26)01316-3 [pii] AID - 10.1016/j.ecoenv.2026.120986 [doi] PST - aheadofprint SO - Ecotoxicol Environ Saf. 2026 Oct 28;326:120986. doi: 10.1016/j.ecoenv.2026.120986. PMID- 42950379 OWN - NLM STAT- Publisher LR - 20261028 IS - 1090-2414 (Electronic) IS - 0147-6513 (Linking) VI - 326 DP - 2026 Oct 28 TI - Integrated assessment of mercury-tolerant plant-associated bacteria from a chlor-alkali site identifies candidates for bioaugmentation. PG - 120984 LID - S0147-6513(26)01314-X [pii] LID - 10.1016/j.ecoenv.2026.120984 [doi] AB - Mercury (Hg)-contaminated environments harbour microorganisms with traits that may be useful for plant-assisted remediation, but high Hg tolerance alone may not identify the most suitable strains for inoculation. In this study, plant-associated bacteria isolated from a chlor-alkali site were evaluated through phenotypic screening, whole-genome analysis, genome-scale metabolic modelling, and willow inoculation assays. Fifty-one Hg-tolerant isolates were recovered, of which 16 were selected for detailed characterization. All selected isolates displayed at least one plant growth-promoting trait, including indole-3-acetic acid production, siderophore production, or phosphate solubilization. Pseudomonas canadensis UFC_B020 and Pseudomonas umsongensis UFC_B021 grew at 200 microM Hg, the highest concentration tested. Genome-based analyses identified putative functions related to metal transport, membrane efflux, oxidative stress responses and plant-beneficial metabolism, while community-scale modelling predicted potential metabolite exchanges within three selected bacterial consortia. In willow inoculation assays, consortium 1 increased root projected area by 5.7% relative to the uninoculated control. The strongest root response was therefore associated with a different consortium from that containing the two isolates with the highest Hg tolerance, highlighting the value of considering both Hg tolerance and plant responses in candidate selection. Together, these results support an integrated strategy combining Hg tolerance, plant growth-promoting traits, genome-based functional information and consortium performance to prioritize site-derived bacteria for subsequent bioaugmentation studies. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Meyer, Lorraine AU - Meyer L AD - Universite Marie et Louis Pasteur, CNRS, Chrono-environnement (UMR 6249), Besancon, F-25000, France. FAU - Zhou, Jun AU - Zhou J AD - Universite Marie et Louis Pasteur, CNRS, Chrono-environnement (UMR 6249), Montbeliard, F-25200, France. Electronic address: jun.zhou@univ-fcomte.fr. FAU - Pfendler, Stephane AU - Pfendler S AD - Universite Marie et Louis Pasteur, CNRS, Chrono-environnement (UMR 6249), Montbeliard, F-25200, France. FAU - Mishra, Akanksha AU - Mishra A AD - IDENER.AI, Biotechnology Division, C/ Gonzalo Jimenez de Quesada, 2, Tower, Floor 11, Seville, 41092, Spain. FAU - Otero-Gonzalez, Lila AU - Otero-Gonzalez L AD - IDENER.AI, Biotechnology Division, C/ Gonzalo Jimenez de Quesada, 2, Tower, Floor 11, Seville, 41092, Spain. FAU - Franco, Marta AU - Franco M AD - IDENER.AI, Biotechnology Division, C/ Gonzalo Jimenez de Quesada, 2, Tower, Floor 11, Seville, 41092, Spain. FAU - Gil-Guerrero, Sara AU - Gil-Guerrero S AD - IDENER.AI, Biotechnology Division, C/ Gonzalo Jimenez de Quesada, 2, Tower, Floor 11, Seville, 41092, Spain. FAU - Meunier, Alexandre AU - Meunier A AD - CHU Besancon Franche-Comte, Hygiene Hospitaliere, Besancon, F-25000, France. FAU - Hocquet, Didier AU - Hocquet D AD - Universite Marie et Louis Pasteur, CNRS, Chrono-environnement (UMR 6249), Besancon, F-25000, France; CHU Besancon Franche-Comte, Hygiene Hospitaliere, Besancon, F-25000, France. FAU - Guyot, Stephane AU - Guyot S AD - Universite Bourgogne Europe, Institut Agro, INRAE, UMR PAM, Dijon, F-21000, France. FAU - Capelli, Nicolas AU - Capelli N AD - Universite Marie et Louis Pasteur, CNRS, Chrono-environnement (UMR 6249), Besancon, F-25000, France. FAU - Chalot, Michel AU - Chalot M AD - Universite Marie et Louis Pasteur, CNRS, Chrono-environnement (UMR 6249), Montbeliard, F-25200, France. LA - eng PT - Journal Article DEP - 20261028 PL - Netherlands TA - Ecotoxicol Environ Saf JT - Ecotoxicology and environmental safety JID - 7805381 SB - IM OTO - NOTNLM OT - Functional screening OT - Genome-scale metabolic modelling OT - Metal tolerance OT - Microbial consortia OT - Rhizosphere COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:01 PHST- 2026/07/17 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:01 [entrez] AID - S0147-6513(26)01314-X [pii] AID - 10.1016/j.ecoenv.2026.120984 [doi] PST - aheadofprint SO - Ecotoxicol Environ Saf. 2026 Oct 28;326:120984. doi: 10.1016/j.ecoenv.2026.120984. PMID- 42950376 OWN - NLM STAT- Publisher LR - 20261028 IS - 1773-0449 (Electronic) IS - 1156-5233 (Linking) VI - 36 IP - 4 DP - 2026 Oct 24 TI - Monoclonal antibodies targeting Candida cell wall and cell-surface antigens: a systematic review. PG - 101664 LID - S1156-5233(26)00065-X [pii] LID - 10.1016/j.mycmed.2026.101664 [doi] AB - BACKGROUND: Invasive candidiasis remains associated with high mortality, while multidrug-resistant species such as Candida auris have increased interest in antibody-based immunotherapies. OBJECTIVE: To systematically evaluate monoclonal antibodies (mAbs) targeting Candida cell wall proteins (CWPs), their mechanisms of protection, and translational challenges. METHODS: PubMed, Scopus, Web of Science, and Embase were searched (2003-2025) following PRISMA 2020 guidelines. Studies reporting characterised mAbs against defined CWP targets with protective data were included. RESULTS: Twelve studies met the inclusion criteria. Ten target categories were identified, including Hwp1, Hyr1, Hsp90, Utr2/Pga31, Ssa1, and beta-1,2-mannoside/mannotriose glycotopes. Protective mechanisms included opsonophagocytosis, biofilm inhibition, immune modulation, and direct disruption of fungal integrity. Cross-species activity against C. auris was reported for several antibodies. Mycograb (anti-Hsp90) is the only passive mAb identified to have reached advanced (Phase III) clinical evaluation; Als3 has reached Phase 2 evaluation via the NDV-3A active-vaccination platform rather than passive mAb therapy. CONCLUSIONS: Candida CWPs represent promising antibody targets, but clinical translation requires antibody humanisation, pharmacokinetic studies, and well-designed combination trials. CI - Copyright (c) 2026 SFMM. Published by Elsevier Masson SAS. All rights reserved. FAU - Kamran, Muhammad AU - Kamran M AD - Department of Allied Health Sciences, Government College Women University Faisalabad, Punjab, Pakistan. Electronic address: kamranahmdani@gmail.com. FAU - Shah, Naseer Ali AU - Shah NA AD - Department of Biosciences, COMSATS University Islamabad, Pakistan. Electronic address: drnaseer@comsats.edu.pk. FAU - Amir, Afreenish AU - Amir A AD - Department of Microbiology, National Institute of Health (NIH), Islamabad, Pakistan. LA - eng PT - Journal Article PT - Review DEP - 20261024 PL - France TA - J Mycol Med JT - Journal de mycologie medicale JID - 9425651 SB - IM OTO - NOTNLM OT - Antifungal immunotherapy OT - Candida albicans OT - Candida auris OT - Cell wall protein OT - Invasive candidiasis OT - Monoclonal antibody OT - Systematic review COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:00 PHST- 2026/06/02 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:00 [entrez] AID - S1156-5233(26)00065-X [pii] AID - 10.1016/j.mycmed.2026.101664 [doi] PST - aheadofprint SO - J Mycol Med. 2026 Oct 24;36(4):101664. doi: 10.1016/j.mycmed.2026.101664. PMID- 42950374 OWN - NLM STAT- Publisher LR - 20261028 IS - 2590-0412 (Electronic) IS - 2590-0412 (Linking) VI - 90 DP - 2026 Oct 28 TI - Invasive RV pressure-volume loops show hemodynamic benefits of reducing lung hyperinflation in COPD. PG - 101330 LID - S2590-0412(26)00084-X [pii] LID - 10.1016/j.resmer.2026.101330 [doi] AB - AIMS: Right heart dysfunction is a common complication in chronic obstructive pulmonary disease (COPD), yet the underlying mechanisms remain poorly understood. Endoscopic lung volume reduction (ELVR) using Zephyr(R) valves improves lung function by reducing emphysema-induced hyperinflation, providing clinical benefits beyond enhanced pulmonary mechanics. Magnetic resonance imaging studies suggest that alleviating lung hyperinflation may also positively affect left heart function. We hypothesized that ELVR may also improve right heart function. RESEARCH QUESTION: This study evaluated right heart function after ELVR in patients with COPD using invasive pressure-volume loop analysis. STUDY DESIGN AND METHODS: Eleven patients with severe COPD underwent baseline right heart catheterization because of suspected pulmonary hypertension, and pressure-volume loop analysis. Eight patients underwent ELVR, followed by right heart function reassessment 12 weeks post-procedure. RESULTS: Pre-ELVR, pulmonary artery pressures ranged from 23 to 32 mmHg, vascular resistance was 2.2-4.8 WU, and significant impaired pressure-volume indexes were observed compared to reference data. Post-ELVR, lung hyperinflation improved significantly, with a reduction in the Residual Volume /Total Lung Capacity (RV/TLC) ratio. RV/TLC changes correlated with improvements in end-diastolic pressure-volume relationships (r=0.82, P=0.02) and the time constant of relaxation Tau (r=0.84, P=0.01). Functional outcomes, such as 6-minute walk distance and dyspnea scores, were driven by stroke work and diastolic parameters, respectively. CONCLUSIONS: In this exploratory single center study, endoscopic lung volume reduction with associated improvement in hyperinflation led to significant improvement in exercise capacity and in right ventricular function. CI - Copyright (c) 2026 SPLF and Elsevier Masson SAS. All rights reserved. FAU - Rohou, Juliette AU - Rohou J AD - PhyMedExp, Department of Respiratory Disease, Univ Montpellier, INSERM, CNRS, CHU, Montpellier, France. FAU - Boissin, Clement AU - Boissin C AD - PhyMedExp, Department of Respiratory Disease, Univ Montpellier, INSERM, CNRS, CHU, Montpellier, France. FAU - Mallet, Jean Pierre AU - Mallet JP AD - PhyMedExp, Department of Respiratory Disease, Univ Montpellier, INSERM, CNRS, CHU, Montpellier, France. FAU - Nogue, Erika AU - Nogue E AD - Clinical research and epidemiology unit, CHU Montpellier, Univ Montpellier, Montpellier, France. FAU - Volpato, Mathilde AU - Volpato M AD - PhyMedExp, Department of Respiratory Disease, Univ Montpellier, INSERM, CNRS, CHU, Montpellier, France. FAU - de Tombe, Pieter AU - de Tombe P AD - PhyMedExp, Department of Respiratory Disease, Univ Montpellier, INSERM, CNRS, CHU, Montpellier, France. FAU - Grillet, Pierre Edouard AU - Grillet PE AD - PhyMedExp, Department of Biochemistry and Hormonology, Univ Montpellier, INSERM, CNRS, CHU, Montpellier, France. FAU - Ahmed, Engi AU - Ahmed E AD - PhyMedExp, Department of Respiratory Disease, Univ Montpellier, INSERM, CNRS, CHU, Montpellier, France. FAU - Molinari, Nicolas AU - Molinari N AD - Clinical research and epidemiology unit, CHU Montpellier, Univ Montpellier, Montpellier, France. FAU - Roubille, Francois AU - Roubille F AD - PhyMedExp, Department of cardiology, Univ Montpellier, INSERM, CNRS; INI-CRT, CHU de, Montpellier, France. FAU - Cazorla, Olivier AU - Cazorla O AD - PhyMedExp, Department of Respiratory Disease, Univ Montpellier, INSERM, CNRS, CHU, Montpellier, France. FAU - Bourdin, Arnaud AU - Bourdin A AD - PhyMedExp, Department of Respiratory Disease, Univ Montpellier, INSERM, CNRS, CHU, Montpellier, France. Electronic address: a-bourdin@chu-montpellier.fr. LA - eng PT - Journal Article DEP - 20261028 PL - France TA - Respir Med Res JT - Respiratory medicine and research JID - 101746324 SB - IM OTO - NOTNLM OT - Emphysema, pulmonary hypertension (PH) OT - Endoscopic lung volume reduction (ELVR) OT - Heart failure with preserved ejection fraction (HFpEF) COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Rohou reports financial support was provided by French Federation of Cardiology. Rohou reports financial support was provided by Apard. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:00 PHST- 2025/11/20 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:00 [entrez] AID - S2590-0412(26)00084-X [pii] AID - 10.1016/j.resmer.2026.101330 [doi] PST - aheadofprint SO - Respir Med Res. 2026 Oct 28;90:101330. doi: 10.1016/j.resmer.2026.101330. PMID- 42950373 OWN - NLM STAT- Publisher LR - 20261028 IS - 0740-2570 (Print) IS - 0740-2570 (Linking) VI - 43 IP - 6 DP - 2026 Sep 29 TI - A comprehensive review on the pathologic evaluation of breast cancer after neoadjuvant systemic therapy. PG - 151060 LID - S0740-2570(26)00080-8 [pii] LID - 10.1016/j.semdp.2026.151060 [doi] AB - Neoadjuvant systemic therapy (NST) is increasingly used across biologic subtypes of breast cancer and includes cytotoxic chemotherapy, HER2-directed therapy, immunotherapy, endocrine therapy, and selected investigational approaches. The expanding therapeutic landscape creates new challenges for pathologists, including recognition of therapy-related morphologic alterations, localization and sampling of the tumor bed, measurement of discontinuous residual invasive carcinoma, assessment of treated lymph nodes, calculation of residual cancer burden (RCB), post-treatment ypTNM staging, and interpretation of biomarkers in residual disease. This review summarizes therapy-associated pathologic changes and provides a practical approach to specimen evaluation and reporting. Emphasis is placed on comparing ypTNM and RCB measurements, diagnostic pitfalls, and circumstances in which repeating biomarker assessment may be clinically informative. Emerging strategies such as image-guided biopsy to identify exceptional responders and preoperative radiotherapy are discussed as newer approaches. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Ismail, Ali AU - Ismail A AD - University of Louisville, Louisville, KY, USA. Electronic address: ali.ismail@louisville.edu. FAU - Sahoo, Sunati AU - Sahoo S AD - Beth Israel Deaconess Medical Center, Department of Pathology, 330 Brookline Avenue, Boston, MA 02215, United States. Electronic address: ssahoo2@bidmc.harvard.edu. LA - eng PT - Journal Article PT - Review DEP - 20260929 PL - United States TA - Semin Diagn Pathol JT - Seminars in diagnostic pathology JID - 8502262 SB - IM OTO - NOTNLM OT - Breast cancer OT - Neoadjuvant chemotherapy OT - Neoadjuvant endocrine therapy OT - Residual cancer burden OT - treatment-related changes COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:00 PHST- 2026/07/07 00:00 [received] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:00 [entrez] AID - S0740-2570(26)00080-8 [pii] AID - 10.1016/j.semdp.2026.151060 [doi] PST - aheadofprint SO - Semin Diagn Pathol. 2026 Sep 29;43(6):151060. doi: 10.1016/j.semdp.2026.151060. PMID- 42950368 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-680X (Electronic) IS - 1761-7727 (Linking) VI - 24 IP - 4S DP - 2026 Oct 28 TI - Physical and mechanical properties of clear plastic retainers: A scoping review. PG - 101253 LID - S1761-7227(26)00120-8 [pii] LID - 10.1016/j.ortho.2026.101253 [doi] AB - AIM: Physical properties refer to a material's observable properties, and mechanical properties relate to a material's response to external forces. This scoping review aims to collate and synthesize the evidence concerning the physical and mechanical properties of clear plastic retainers (CPRs). METHODS: Six databases and a grey literature source were searched with key terms regarding CPRs and their physical and mechanical properties. The study followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses Extension for Scoping Reviews. The findings were categorized into seven sections by mechanical and/or physical property. RESULTS: The initial search yielded 1086 studies with forty-three satisfying selection criteria. Five related to three-dimensional (3D)-printed CPRs. The remainder concerned 'traditionally' fabricated, thermoplastic CPRs (T-CPRs). Nine thermoplastic materials and three 3D-printed materials were evaluated with a total of 36 commercial brands being investigated. 3D-CPRs generally displayed inferior optical and mechanical properties compared to T-CPRs although post-processing generally appeared to enhance relevant properties. Curry and coffee were frequently associated with staining. Cleaning agents containing sodium hypochlorite and tooth-whitening agents containing peroxides appeared to negatively affect surface roughness and mechanical properties. CONCLUSION: 3D-CPRs displayed inferior optical and mechanical properties compared to T-CPRs. Patients should be advised to avoid using bleach-containing agents to clean their CPRs and avoid using their CPRs as receptacles for tooth-whitening materials. They should be made aware of the staining-potential of coffee and curry on the appliance. Further relevant clinical research over the long term is required. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Masson SAS.. All rights reserved. FAU - Sidhu, Leah M AU - Sidhu LM AD - Orthodontic Unit, School of Dentistry, Adelaide University, Adelaide, South Australia, Australia. FAU - Meade, Maurice J AU - Meade MJ AD - Orthodontic Unit, School of Dentistry, Adelaide University, Adelaide, South Australia, Australia. Electronic address: maurice.meade@adelaide.edu.au. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - France TA - Int Orthod JT - International orthodontics JID - 101184882 SB - IM OTO - NOTNLM OT - Clear retainers OT - Essix retainers OT - Mechanical properties OT - Orthodontic retainers OT - Physical properties OT - Vacuum formed retainers COIS- Disclosure of interest The authors declare that they have no competing interest. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:00 PHST- 2026/08/06 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:00 [entrez] AID - S1761-7227(26)00120-8 [pii] AID - 10.1016/j.ortho.2026.101253 [doi] PST - aheadofprint SO - Int Orthod. 2026 Oct 28;24(4S):101253. doi: 10.1016/j.ortho.2026.101253. PMID- 42950367 OWN - NLM STAT- Publisher LR - 20261028 IS - 0151-9638 (Print) IS - 0151-9638 (Linking) VI - 153 IP - 4 DP - 2026 Oct 28 TI - Protected from onychomycosis: selective sparing of an overlapped toenail. PG - 103536 LID - S0151-9638(26)00070-0 [pii] LID - 10.1016/j.annder.2026.103536 [doi] FAU - Podolsky, A S AU - Podolsky AS AD - Renaissance School of Medicine, Stony Brook University, NY, USA. FAU - Lipner, S R AU - Lipner SR AD - Department of Dermatology, Weill Cornell Medicine, NY, USA. Electronic address: shl9032@med.cornell.edu. LA - eng PT - Journal Article DEP - 20261028 PL - France TA - Ann Dermatol Venereol JT - Annales de dermatologie et de venereologie JID - 7702013 SB - IM COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 19:00 PHST- 2026/04/28 00:00 [received] PHST- 2026/08/26 00:00 [accepted] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 19:00 [entrez] AID - S0151-9638(26)00070-0 [pii] AID - 10.1016/j.annder.2026.103536 [doi] PST - aheadofprint SO - Ann Dermatol Venereol. 2026 Oct 28;153(4):103536. doi: 10.1016/j.annder.2026.103536. PMID- 42950356 OWN - NLM STAT- Publisher LR - 20261028 IS - 2057-1976 (Electronic) IS - 2057-1976 (Linking) DP - 2026 Oct 28 TI - On the feasibility of temporal interference stimulation of human brains using two arrays of electrodes. LID - 10.1088/2057-1976/aeba92 [doi] AB - Temporal interference (TI) stimulation leverages two pairs of electrodes to induce an interfering electrical field in the brain. Studies show that TI can stimulate the deep brain without affecting shallow areas. Optimal placement of these electrodes for maximal focal modulation remains computationally challenging. We are the first to propose two arrays instead of two or multiple pairs of electrodes to boost modulation focality. However, it outputs too many electrodes with overlaps across two frequencies, making it difficult to implement in practice. Objective: Based on recent progress in developing multi-channel TI devices and computational work, here we advocate two-array TI with feasibility data. Methods & Results: We review TI optimization algorithms, and compare them over 25 heads across six brain targets. We show that the latest optimization algorithm for two-pair TI innately works for two-array TI with the fastest speed (under 30s) and a similar amount of electrodes as in multi-pair TI. At four of the six targets, this fastest algorithm for two-array TI achieves similar or even better focality than TI using 16 pairs of electrodes that takes days to optimize. We show a hardware implementation of two-array TI on our 8-channel TI device. We argue that two-pair TI is only preferred for maximal modulation strength or when hardware is limited to two current sources. We restate the focality-intensity tradeoff in the context of TI and provide a first voxel-level map (at 4 mm resolution) of achievable TI focality and modulation strength in the MNI-152 head template. Conclusions: Compared to two-pair or multi-pair TI, we promote two-array TI for its similar performance in focality and lower cost in both optimization time and electrodes needed. We hope this work will pave the way for future adoptions of two-array TI for more focal non-invasive deep brain stimulation. CI - (c) 2026 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved. FAU - Huang, Yu AU - Huang Y AUID- ORCID: 0000-0003-4178-0739 AD - Research and Development, Soterix Medical Inc, 1480 US Highway 9 North, #204, Woodbridge Township, New Jersey, 07095, United States. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Biomed Phys Eng Express JT - Biomedical physics & engineering express JID - 101675002 SB - IM OTO - NOTNLM OT - computational models OT - model optimization OT - multi-channel TI stimulator OT - noninvasive deep brain stimulation OT - temporal interference stimulation EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 18:53 PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 18:53 [entrez] AID - 10.1088/2057-1976/aeba92 [doi] PST - aheadofprint SO - Biomed Phys Eng Express. 2026 Oct 28. doi: 10.1088/2057-1976/aeba92. PMID- 42950354 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1449-8987 (Electronic) IS - 1448-5028 (Linking) VI - 23 IP - 6 DP - 2026 Dec 17 TI - Body mass index and major surgical complications in outpatient surgical abortion: an Australia-wide case-control study. LID - SH26025 [pii] LID - 10.1071/SH26025 [doi] AB - BACKGROUND: Existing evidence on the association between body mass index (BMI) and surgical abortion complications is inconclusive and derives exclusively from retrospective cohort studies conducted in the United States of America, often underpowered for uncommon surgical outcomes. In Australia, BMI is commonly considered when determining eligibility for outpatient surgical abortion, yet no Australian data have examined its association with major surgical complications. METHODS: We conducted a retrospective case-control study across 10 oupatient surgical abortion clinics operated by MSI Australia between 2016 and 2024. Cases were procedures complicated by major surgical complications (haemorrhage >/=500 mL, uterine perforation, hospital transfer, laparoscopy, laparotomy, disseminated intravascular coagulation, intensive care admission, blood transfusion, or hysterectomy), identified through mandatory incident reporting. Four gestation-matched controls were selected per case. BMI was analysed as a continuous variable, as obesity (>/=30 kg/m2), and by ordered categories. Multivariable logistic regression adjusted for parity, gestational age and previous caesarean section. RESULTS: BMI data were available for 775 of 795 individuals (159 cases, 636 controls). Mean BMI was slightly higher among cases than controls (25.9 vs 24.8 kg/m2; P = 0.017), but distributions were similar. Crude associations between BMI, obesity and complications did not persist after adjustment (per-unit increase: adjusted odds ratio (aOR) 1.01, 95% confidence interval [CI] 0.97-1.04; obesity: aOR 1.24, 95% CI 0.78-1.96). CONCLUSIONS: BMI was not an independent predictor of major surgical complications in outpatient surgical abortion within the BMI range represented. Risk stratification should prioritise gestational age, parity and previous caesarean section rather than BMI alone, noting limited data for very high BMI categories. CI - (c) 2026 The Author(s) (or their employer(s)). Published by CSIRO Publishing. FAU - Drever, Natalie AU - Drever N AUID- ORCID: 0000-0003-3906-8769 AD - Cairns Clinical School, College of Medicine and Dentistry, James Cook University, Cairns Hospital, Level 2, A Block, The Esplanade, Cairns North, Qld 4878, Australia. AD - Department of Obstetrics and Gynaecology, Cairns Hospital, Cairns, Qld, Australia. FAU - Johnston, Liam AU - Johnston L AD - Cairns Clinical School, College of Medicine and Dentistry, James Cook University, Cairns Hospital, Level 2, A Block, The Esplanade, Cairns North, Qld 4878, Australia. FAU - O'Brien, Cecelia AU - O'Brien C AD - College of Medicine and Dentistry, James Cook University, Townsville City, Qld, Australia. FAU - Melville, Catriona AU - Melville C AUID- ORCID: 0000-0001-5891-0259 AD - Department of Obstetrics and Gynaecology, Logan Hospital, Loganholme, Qld, Australia. FAU - Black, Kirsten AU - Black K AD - Reproduction and Perinatal Centre, Faculty of Medicine and Health, University of Sydney, Camperdown, NSW, Australia. FAU - Goldstone, Philip AU - Goldstone P AD - MSI Australia, Sydney, Qld, Australia. FAU - de Costa, Caroline AU - de Costa C AD - The Cairns Institute, James Cook University, Cairns, Qld, Australia. LA - eng PT - Journal Article PL - Australia TA - Sex Health JT - Sexual health JID - 101242667 SB - IM MH - Humans MH - Female MH - *Body Mass Index MH - Case-Control Studies MH - *Abortion, Induced/adverse effects MH - Australia/epidemiology MH - Adult MH - Retrospective Studies MH - *Postoperative Complications/epidemiology MH - Pregnancy MH - *Obesity/complications/epidemiology MH - Risk Factors OTO - NOTNLM OT - BMI OT - abortion care OT - case-control study OT - dilation and curettage OT - dilation and evacuation OT - obesity OT - reproductive health services OT - surgical abortion EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 18:53 PHST- 2026/01/22 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 18:53 [entrez] AID - 278999 [pii] AID - 10.1071/SH26025 [doi] PST - ppublish SO - Sex Health. 2026 Dec 17;23(6):SH26025. doi: 10.1071/SH26025. PMID- 42950350 OWN - NLM STAT- Publisher LR - 20261028 IS - 2057-1976 (Electronic) IS - 2057-1976 (Linking) DP - 2026 Oct 28 TI - Learning discriminative time-frequency representations for EEG segment-level seizure-state classification with channel selection. LID - 10.1088/2057-1976/aeba93 [doi] AB - Abstract Electroencephalography (EEG)-based seizure monitoring supports the diagnosis and management of epilepsy; however, high-dimensional multichannel EEG recordings impose substantial computational burden and limit suitability for portable monitoring systems. This paper proposes a reduced-channel framework for segment-level classification of EEG into ictal, preictal and interictal states. It integrates attention-guided EEG channel selection, high-resolution time-frequency analysis via the Time Reassigned MultiSynchroSqueezing Transform (TMSST) and Concentration of Frequency and Time (ConceFT), and a hybrid CNN-BiLSTM classifier. The framework is evaluated on the CHB-MIT and Siena Scalp EEG databases under two complementary protocols: a stratified 70/30 holdout split and rigorous Leave-One-Subject-Out (LOSO) cross-validation to assess subject independent generalization. Under holdout evaluation using a single EEG channel, the proposed framework achieves an accuracy of 99.28 +/- 0.63%, sensitivity of 99.18%, specificity of 99.64%, and MCC of 0.97 on CHB-MIT, and an accuracy of 98.75 +/- 0.74%, sensitivity of 98.77%, specificity of 99.38%, and MCC(Matthews correlation coefficients) of 0.97 on Siena. LOSO cross-validation demonstrates subject independent generalization, exceeding 91% accuracy on both databases. These results demonstrate that the framework achieves an effective balance among segment-level classification accuracy, computational efficiency and reduced-channel operation. Moreover, applicability for portable and wearable EEG-based seizure-state monitoring applications is investigated. CI - (c) 2026 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved. FAU - Ghosh, Antara AU - Ghosh A AUID- ORCID: 0000-0001-9468-253X AD - Jadavpur University, Kolkata, Kolkata, 700032, India. FAU - Dey, Debangshu AU - Dey D AUID- ORCID: 0000-0002-7163-628X AD - Jadavpur University, Kolkata, Kolkata, 700032, India. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Biomed Phys Eng Express JT - Biomedical physics & engineering express JID - 101675002 SB - IM OTO - NOTNLM OT - BiLSTM OT - CNN OT - ConceFT OT - EEG OT - Seizure-State Classification OT - TMSST EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 18:52 PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 18:52 [entrez] AID - 10.1088/2057-1976/aeba93 [doi] PST - aheadofprint SO - Biomed Phys Eng Express. 2026 Oct 28. doi: 10.1088/2057-1976/aeba93. PMID- 42950323 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261028 IS - 1715-1635 (Print) IS - 1715-1635 (Linking) VI - 159 IP - S2 DP - 2026 Jul-Aug TI - Community-based blood pressure screening in Newfoundland and Labrador: Results from the 2024 May Measurement Month campaign. PG - S11-S19 LID - 10.3138/cpj-25-0078 [doi] AB - BACKGROUND: May Measurement Month (MMM) is an annual, global blood pressure (BP) screening campaign that aims to raise awareness of the risks associated with hypertension. During MMM 2024, community pharmacies across Newfoundland and Labrador (NL) participated in the initiative, contributing BP screening data to local, national, and international research efforts. The primary objective of this study was to assess the local burden of elevated BP among those with and without known hypertension. METHODS: This cross-sectional study followed a modified version of the MMM protocol. All consenting adults 18 years or older were eligible to participate. Data were collected at 25 community pharmacies in NL. Descriptive statistics were used to summarize data; logistic regression was used to identify associations between elevated BP and selected covariates. RESULTS: Among 220 participants (mean age 53.3 +/- 17.8 years), 68.2% were female, and 40.9% had known hypertension. Analysis included 208 participants with 3 BP readings. Elevated BP was observed in 25.5%, including 18.3% without known hypertension and 36.9% taking antihypertensives. Body mass index and diabetes were statistically significant predictors of elevated BP in the multivariate model. DISCUSSION: The proportion of participants in this study with elevated BP exceeds national statistics. The large number of participants with elevated BP and known hypertension and/or diabetes suggests that improvements are needed in the primary health care setting to support patients in reaching their BP targets. CONCLUSION: Community pharmacists in NL uncovered large numbers of patients with elevated BP as part of MMM 2024, demonstrating the success of this community-based initiative. FAU - Coombs, Stephen D AU - Coombs SD AD - Memorial University School of Pharmacy, St. John's, NL. FAU - Young, Stephanie W AU - Young SW AD - Memorial University School of Pharmacy, St. John's, NL. FAU - Tsuyuki, Ross T AU - Tsuyuki RT AD - University of Alberta Faculty of Medicine and Dentistry, Edmonton, AB. FAU - Lee, Tiffany A AU - Lee TA AD - Memorial University School of Pharmacy, St. John's, NL. LA - eng PT - Journal Article DEP - 20260720 PL - Canada TA - Can Pharm J (Ott) JT - Canadian pharmacists journal : CPJ = Revue des pharmaciens du Canada : RPC JID - 101257110 EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 17:43 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 17:43 [entrez] AID - 10.3138/cpj-25-0078 [doi] PST - ppublish SO - Can Pharm J (Ott). 2026 Jul-Aug;159(S2):S11-S19. doi: 10.3138/cpj-25-0078. Epub 2026 Jul 20. PMID- 42950319 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261028 IS - 1715-1635 (Print) IS - 1715-1635 (Linking) VI - 159 IP - 3 DP - 2026 May-Jun TI - Barriers and facilitators to antimicrobial stewardship in community pharmacy: A systematic review. PG - 202-213 LID - 10.3138/cpj-25-0045 [doi] AB - BACKGROUND: Community pharmacists (CPs) are well-positioned to address the threat of antimicrobial resistance (AMR) by encouraging appropriate antimicrobial use. Understanding the individual and contextual determinants of CPs' involvement in antimicrobial stewardship (AMS) helps select sustainable strategies. Our objective was to identify and classify the barriers and facilitators to CPs' involvement in AMS. METHODS: We conducted a systematic review of studies that evaluated the determinants of CP recommending, prescribing, or counselling on appropriate antibiotic use. We searched 5 databases (Ovid Medline, Embase, International Pharmaceutical Abstracts, PsycINFO, and Ebsco CINAHL) from inception to April 24, 2024, using search concepts of community pharmacy and AMS. Title and abstract, as well as full-text screening, were performed by 2 reviewers. Risk of bias was assessed using a modified Critical Appraisal Skills Programme tool. We extracted study details, with barriers and facilitators categorized according to the Theoretical Domains Framework and Consolidated Framework for Implementation Research. RESULTS: Of the 2429 studies screened, 40 were included. High-income countries constituted 26 studies while 14 were in low-middle-income countries. Nine evaluated a specific AMS initiative. Key barriers included lack of time to participate in AMS, lack of collaboration with physicians, and lack of access to patient records. Facilitators included financial incentives and reimbursement for service, specialized training opportunities, and guidelines for AMS practice. CONCLUSION: This review identified key barriers and facilitators to antimicrobial stewardship in community pharmacy. These determinants can form the basis for selecting impactful community pharmacy AMS strategies to reduce the burden of AMR. FAU - Vigneswaran, Shankavi AU - Vigneswaran S AD - Dalla Lana School of Public Health, University of Toronto, Toronto, ON. FAU - Langford, Bradley J AU - Langford BJ AD - Dalla Lana School of Public Health, University of Toronto, Toronto, ON. AD - Hotel Dieu Shaver Health and Rehabilitation Centre, St. Catharines, ON. FAU - Brown, Kevin AU - Brown K AD - Dalla Lana School of Public Health, University of Toronto, Toronto, ON. FAU - Daneman, Nick AU - Daneman N AD - Institute of Health Policy, Management and Evaluation, University of Toronto, ON. AD - Sunnybrook Health Sciences Centre, Toronto, ON. AD - Temerty Faculty of Medicine, University of Toronto, ON. FAU - Ha, Rita AU - Ha R AD - North York Family Health Team, North York, ON. FAU - Ho, Certina AU - Ho C AD - ISMP Canada, Toronto, ON. AD - Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON. FAU - Ko, Lina AU - Ko L AD - Independent pharmacy, Markham, ON. FAU - Leung, Valerie AU - Leung V AD - Michael Garron Hospital, Toronto East Health Network, Toronto, ON. FAU - Levinson, Wendy AU - Levinson W AD - Choosing Wisely Canada, Toronto, ON. FAU - Maximos, Mira AU - Maximos M AD - Women's College Hospital, Toronto, ON. FAU - Nekolaichuk, Erica AU - Nekolaichuk E AD - University of Toronto Libraries, Gerstein Science Information Centre, University of Toronto, Toronto, ON. FAU - Neudorf, Kim AU - Neudorf K AD - Patients for Patient Safety Canada, Edmonton, AB. FAU - Ng, Angeline AU - Ng A AD - Ontario Pharmacists Association, Toronto, ON. FAU - Papastergiou, John AU - Papastergiou J AD - Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON. AD - Shoppers Drug Mart, Toronto, ON. FAU - Raybardhan, Sumit AU - Raybardhan S AD - Dalla Lana School of Public Health, University of Toronto, Toronto, ON. AD - North York General Hospital, Toronto, ON. FAU - Schwartz, Kevin L AU - Schwartz KL AD - Temerty Faculty of Medicine, University of Toronto, ON. AD - Unity Health Toronto, Toronto, ON. FAU - Smith, Maureen AU - Smith M AD - Patient partner, Toronto, ON. FAU - Szymczak, Julia E AU - Szymczak JE AD - University of Utah School of Medicine, Salt Lake City, Utah, USA. FAU - Tadrous, Mina AU - Tadrous M AD - Women's College Hospital, Toronto, ON. FAU - Tam, Kathy AU - Tam K AD - Ontario Pharmacists Association, Toronto, ON. FAU - Warshafsky, Daniel AU - Warshafsky D AD - Dalla Lana School of Public Health, University of Toronto, Toronto, ON. AD - Ontario Ministry of Health, Toronto, ON. FAU - So, Miranda AU - So M AD - Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON. AD - Toronto General Hospital Research Institute, Toronto, ON. AD - University Health Network, Toronto, ON. AD - University of Melbourne, Melbourne, Australia. LA - eng PT - Journal Article DEP - 20260409 PL - Canada TA - Can Pharm J (Ott) JT - Canadian pharmacists journal : CPJ = Revue des pharmaciens du Canada : RPC JID - 101257110 EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 17:43 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 17:43 [entrez] AID - 10.3138/cpj-25-0045 [doi] PST - ppublish SO - Can Pharm J (Ott). 2026 May-Jun;159(3):202-213. doi: 10.3138/cpj-25-0045. Epub 2026 Apr 9. PMID- 42950276 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261028 IS - 1715-1635 (Print) IS - 1715-1635 (Linking) VI - 159 IP - 2 DP - 2026 Mar-Apr TI - Peripartum mental health education in Canadian pharmacy curricula. PG - 111-119 LID - 10.3138/cpj-25-0034 [doi] AB - BACKGROUND: Peripartum mental health conditions are common in Canada, yet many women lack proper support. Pharmacists represent a potentially underused resource for these patients, but it is unclear whether Canadian PharmD programs adequately train future pharmacists to address this need. This study aimed to assess peripartum mental health instruction in Canadian PharmD programs. METHODS: A national cross-sectional survey was conducted during November 2023 to August 2024. It was distributed via email by members of the Association of Faculties of Pharmacy of Canada to a representative from each pharmacy school. The survey included 34 questions covering curriculum, course status, content, and instructional methodology. Data were analyzed using descriptive statistics. RESULTS: The survey response rate was 60% (6/10 schools). Respondents agreed that pharmacists can play a critical role in supporting peripartum patients with mental illness. While most schools included topics such as peripartum depression and anxiety, many felt that peripartum mental health content was insufficiently covered. Most schools dedicated 1-3 hours to this topic, primarily through lectures. Two schools included laboratory components, such as simulated patient interviews. No school had a standalone course on peripartum mental health care. Respondents expressed difficulty fitting this content into an overloaded curriculum. Topics considered essential were appropriate therapy selection, patient-centred communication, and peripartum depression. CONCLUSIONS: This survey highlights that while peripartum mental health is recognized as a potentially important area of pharmacy practice, its coverage in Canadian PharmD programs is limited. Future research should clarify the opportunities for pharmacists in peripartum mental health care and their related educational needs. FAU - Urslak, Randilynne AU - Urslak R AD - School of Pharmacy, University of Waterloo, Waterloo, ON. AD - School of Pharmaceutical Sciences, University of Ottawa, Ottawa, ON. FAU - Deng, Catherine AU - Deng C AD - School of Pharmacy, University of Waterloo, Waterloo, ON. FAU - Nakhla, Nardine AU - Nakhla N AD - School of Pharmacy, University of Waterloo, Waterloo, ON. FAU - Richard, Cynthia AU - Richard C AD - School of Pharmacy, University of Waterloo, Waterloo, ON. FAU - Maxwell, Colleen J AU - Maxwell CJ AD - School of Pharmacy, University of Waterloo, Waterloo, ON. LA - eng PT - Journal Article DEP - 20260217 PL - Canada TA - Can Pharm J (Ott) JT - Canadian pharmacists journal : CPJ = Revue des pharmaciens du Canada : RPC JID - 101257110 EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:28 CRDT- 2026/10/28 17:33 PHST- 2026/10/28 23:28 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 17:33 [entrez] AID - 10.3138/cpj-25-0034 [doi] PST - ppublish SO - Can Pharm J (Ott). 2026 Mar-Apr;159(2):111-119. doi: 10.3138/cpj-25-0034. Epub 2026 Feb 17. PMID- 42950254 OWN - NLM STAT- Publisher LR - 20261028 IS - 1552-4175 (Electronic) IS - 1099-8004 (Linking) DP - 2026 Oct 28 TI - Acute and Chronic Cardiovascular and Neuromuscular Effects of Blood Flow Restriction Resistance Training in Older Adults: A Systematic Review and Meta-Analysis. PG - 10998004261502747 LID - 10.1177/10998004261502747 [doi] AB - Low-load blood flow restriction resistance training (BFR-RT) may offer a lower-load alternative to conventional resistance training for older adults, but its cardiovascular responses and neuromuscular benefits remain incompletely characterized. To synthesize the acute cardiovascular responses and chronic cardiovascular and neuromuscular effects of low-load BFR-RT in older adults. PubMed/MEDLINE, Scopus, Web of Science, CENTRAL, and CINAHL were searched from inception to July 15, 2026. Randomized controlled trials, including parallel-group and crossover designs, comparing low-load BFR-RT with no-exercise/passive control or conventional non-BFR resistance training were eligible. Risk of bias was assessed using the Cochrane RoB 2 tool. Meta-analyses were performed using mean differences (MDs) with 95% confidence intervals (CIs). Twenty-seven studies were included. Acutely, BFR-RT increased systolic blood pressure versus no exercise (MD, 5.30 mmHg; 95% CI, 0.48 to 10.12) and increased diastolic blood pressure (MD, 5.23 mmHg) and mean arterial pressure (MD, 5.81 mmHg) versus conventional resistance training, while heart rate, stroke volume, and cardiac output were not significantly different. Chronically, resting cardiovascular and endothelial outcomes were largely unchanged. Versus no exercise, BFR-RT significantly improved knee-extension, leg-extension, and leg-press 1RM and quadriceps cross-sectional area. Comparisons with conventional resistance training were mixed and outcome-specific. Low-load BFR-RT improves selected neuromuscular outcomes in older adults, particularly relative to no exercise, while cardiovascular responses are comparator-dependent and chronic vascular effects appear largely neutral. These findings support cautious, individualized application rather than assumptions of cardiovascular superiority or equivalence to conventional resistance training. FAU - Norouzi, Kazem AU - Norouzi K AD - Department of Sports Injuries and Corrective Exercises, Ramo Health Club, Mashhad, Iran. FAU - Nobari, Hadi AU - Nobari H AD - LFE Research Group, Department of Health and Human Performance, Faculty of Physical Activity and Sport Science (INEF), Universidad Politecnica de Madrid, Madrid, Spain. RINGGOLD: 16771 AD - Department of Sports Medicine, Sport Sciences Research Institute, Tehran, Iran. FAU - Jamali Masoomi, Samira AU - Jamali Masoomi S AD - Department of Sport Sciences, Faculty of Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran. RINGGOLD: 48440 FAU - Saberisani, Reza AU - Saberisani R AD - Department of Health and Sport Rehabilitation, Faculty of Sport Sciences and Health, Shahid Beheshti University, Tehran, Iran. RINGGOLD: 48512 FAU - Niyazi, Arghavan AU - Niyazi A AD - Department of Exercise Physiology, Ayandegan-e-Sharq Healthcare Center, Mashhad, Iran. FAU - Mohammad Rahimi, Gholam Rasul AU - Mohammad Rahimi GR AUID- ORCID: 0000-0002-8159-2065 AD - Department of Exercise Physiology, Ayandegan-e-Sharq Healthcare Center, Mashhad, Iran. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Biol Res Nurs JT - Biological research for nursing JID - 9815758 SB - IM OTO - NOTNLM OT - blood flow restriction training OT - hemodynamic responses OT - muscle strength OT - older adults OT - resistance exercise EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 17:23 PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 17:23 [entrez] AID - 10.1177/10998004261502747 [doi] PST - aheadofprint SO - Biol Res Nurs. 2026 Oct 28:10998004261502747. doi: 10.1177/10998004261502747. PMID- 42950253 OWN - NLM STAT- Publisher LR - 20261028 IS - 2473-9537 (Electronic) IS - 2473-9529 (Linking) DP - 2026 Oct 28 TI - Omission of Radiation Therapy is Associated with Inferior Disease Control in High Risk Early-Stage Hodgkin Lymphoma. LID - bloodadvances.2026021839 [pii] LID - 10.1182/bloodadvances.2026021839 [doi] AB - Early-stage classical Hodgkin lymphoma (HL) is highly curable, yet optimal frontline treatment remains contested. Combined modality therapy (CMT) with chemotherapy followed by radiotherapy (RT) has been the standard, but concern over RT-associated toxicities has driven adoption of chemotherapy-alone approaches despite randomized trials failing to demonstrate noninferiority with RT omission. We analyzed 810 adult patients treated across eight academic centers for stage I/II HL between 1/2012 and 1/2022, comparing outcomes with CMT and chemotherapy alone. Primary endpoints were progression-free (PFS) and overall survival (OS); propensity-score matching was conducted to homogenize baseline characteristics between the groups, and subgroup analyses were conducted to identify clinical features associated with differential benefit. Among 480 propensity-matched patients, five-year OS was 98.6% with CMT versus 97.3% with chemotherapy alone (p=0.620); five-year PFS was 87.2% vs 81.4% (p=0.053). CMT conferred significantly superior PFS in patients with bulky disease (91.8% vs 73.0%; HR 3.77; p=0.005) and interim (iPET)-positive disease (Deauville score (DS)>/=4; 74.9% vs 51.9%; HR 2.97; p=0.020). iPET response was the strongest independent predictor of both progression (HR 7.02) and mortality (HR 6.97), with PFS declining markedly across DS groups (DS1-2: 92.2%; DS3: 79.4%; DS4-5: 46.4%). Among iPET-positive patients, those receiving unplanned consolidative RT required less salvage therapy than those managed with chemotherapy escalation alone (29.0% vs. 45.2%; p=0.037). Late toxicities were more frequent with CMT, though 69% of second malignancies occurred outside the RT field. These findings support risk-adapted, biomarker-driven treatment selection and the need for prospective trials incorporating modern RT techniques and iPET-adapted algorithms. CI - Copyright (c) 2026 American Society of Hematology. FAU - Dave, Nikita K AU - Dave NK AD - Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania, United States. FAU - Dreyfuss, Alexandra Danielle AU - Dreyfuss AD AD - University of Miami, Miami, Florida, United States. FAU - Moskowitz, Alison J AU - Moskowitz AJ AD - Memorial Sloan Kettering Cancer Center, New York, New York, United States. FAU - Yahalom, Joachim AU - Yahalom J AD - Memorial Sloan-Kettering Cancer Center, New York, New York, United States. FAU - Alfaro, Maria Katerina C AU - Alfaro MKC AD - Perelman School of Medicine, Philadelphia, Pennsylvania, United States. FAU - Birrer, Nicole AU - Birrer N AD - University of Utah, Salt Lake City, Utah, United States. FAU - Hu, Boyu AU - Hu B AD - Huntsman Cancer Institute/University of Utah, Salt Lake City, Utah, United States. FAU - Koch, Rachel AU - Koch R AD - Fox Chase Cancer Center, Philadelphia, Pennsylvania, United States. FAU - Frosch, Zachary Ak AU - Frosch ZA AUID- ORCID: 0000-0002-4599-0533 AD - Fox Chase Cancer Center, Philadelphia, Pennsylvania, United States. FAU - Colton, Meryl AU - Colton M AD - University of California San Francisco, San Francisco, California, United States. FAU - Ho, Carrie AU - Ho C AD - Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, California, United States. FAU - Amoozgar, Behzad AU - Amoozgar B AD - Hackensack University Medical Center, United States. FAU - Feldman, Tatyana A AU - Feldman TA AD - Hackensack University Medical Center, Hackensack, New Jersey, United States. FAU - Saxena, Abhiraj AU - Saxena A AUID- ORCID: 0009-0009-7338-2269 AD - Division of Blood Disorders, Rutgers Cancer Institute, New Brunswick, NJ, Brunswick, New Jersey, United States. FAU - Rhodes, Joanna M AU - Rhodes JM AD - Rutgers Cancer Institute, New Brunswick, New Jersey, United States. FAU - Dunton, Ashley AU - Dunton A AUID- ORCID: 0009-0002-4214-9359 AD - Medical College of Wisconsin Cancer Center, United States. FAU - Shah, Nirav N AU - Shah NN AUID- ORCID: 0000-0002-4336-1071 AD - Medical College of Wisconsin, Wauwatosa, Wisconsin, United States. FAU - Mantione, Amelia AU - Mantione A AD - Drexel University College of Medicine, Philadelphia, Pennsylvania, United States. FAU - Hubbeling, Harper AU - Hubbeling H AD - University of Pennsylvania, Philadelphia, Pennsylvania, United States. FAU - LaRiviere, Michael J AU - LaRiviere MJ AUID- ORCID: 0000-0002-1662-5086 AD - University of Pennsylvania, Philadelphia, Pennsylvania, United States. FAU - Plastaras, John P AU - Plastaras JP AD - University of Pennsylvania, Philadelphia, Pennsylvania, United States. FAU - Fregonese, Beatrice AU - Fregonese B AD - Memorial Sloan Kettering Cancer Center, New York, New York, United States. FAU - Imber, Brandon S AU - Imber BS AUID- ORCID: 0000-0002-1281-5915 AD - Memorial Sloan Kettering Cancer Center, New York, New York, United States. FAU - Muenzen, Reid M AU - Muenzen RM AD - Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania, United States. FAU - Barta, Stefan K AU - Barta SK AUID- ORCID: 0000-0002-3280-5271 AD - Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania, United States. FAU - Carter, Jordan AU - Carter J AD - University of Pennsylvania, Philadelphia, Pennsylvania, United States. FAU - Chong, Elise A AU - Chong EA AUID- ORCID: 0000-0001-8895-1625 AD - Lymphoma Program, Abramson Cancer Center, University of Pennsylvania, Philadelphia, Pennsylvania, United States. FAU - Cook, Michael AU - Cook M AUID- ORCID: 0000-0002-6171-9647 AD - University of Pennsylvania, Philadelphia, Pennsylvania, United States. FAU - Landsburg, Daniel J AU - Landsburg DJ AD - University of Pennsylvania, Philadelphia, Pennsylvania, United States. FAU - Nasta, Sunita D AU - Nasta SD AD - University of Pennsylvania, Philadelphia, Pennsylvania, United States. FAU - Schuster, Stephen J AU - Schuster SJ AUID- ORCID: 0000-0002-3376-8978 AD - Lymphoma Program, Abramson Cancer Center, University of Pennsylvania, Philadelphia, Pennsylvania, United States. FAU - Thomas, Colin J AU - Thomas CJ AD - University of Pennsylvania, Philadelphia, Pennsylvania, United States. FAU - Tomasulo, Emily AU - Tomasulo E AD - University of Pennsylvania, Washington Crossing, Pennsylvania, United States. FAU - Svoboda, Jakub AU - Svoboda J AD - University of Pennsylvania, Philadelphia, Pennsylvania, United States. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Blood Adv JT - Blood advances JID - 101698425 SB - IM EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 17:14 PHST- 2026/09/22 00:00 [accepted] PHST- 2026/07/16 00:00 [received] PHST- 2026/09/04 00:00 [revised] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 17:14 [entrez] AID - 571810 [pii] AID - 10.1182/bloodadvances.2026021839 [doi] PST - aheadofprint SO - Blood Adv. 2026 Oct 28:bloodadvances.2026021839. doi: 10.1182/bloodadvances.2026021839. PMID- 42950249 OWN - NLM STAT- Publisher LR - 20261028 IS - 1553-3514 (Electronic) IS - 1553-3506 (Linking) DP - 2026 Oct 28 TI - Dynamic Machine Learning Prediction of Colorectal Anastomotic Leak Using Preoperative Factors and Serial Postoperative Biomarker Trajectories. PG - 15533506261501663 LID - 10.1177/15533506261501663 [doi] AB - AimAnastomotic leak remains a life-threatening complication after colorectal surgery. Static preoperative models show only modest discrimination, whereas clinical signs often appear late. We aimed to develop and internally validate dynamic machine-learning models integrating preoperative factors with serial postoperative biomarker and physiological trajectories.MethodIn this retrospective single-centre cohort, XGBoost models were developed using information available preoperatively and through POD1, POD3, or POD5. For the POD3 and POD5 models, patients with AL diagnosed by the respective time point were excluded so that each model estimated the risk of a subsequent leak. Internal validation used repeated stratified 5-fold cross-validation with 5 repeats, with preprocessing and threshold selection performed within the training data.ResultsAmong 409 patients, 31 (7.6%) developed AL. AUROC was 0.696 +/- 0.085 preoperatively and 0.714 +/- 0.083 at POD1. At POD3, 405 patients remained at risk and 27 subsequently developed AL; AUROC was 0.781 +/- 0.086. At POD5, 398 patients remained at risk and 20 subsequently developed AL; AUROC was 0.908 +/- 0.047 and AUPRC 0.460 +/- 0.152, with sensitivity 0.850 +/- 0.177, specificity 0.792 +/- 0.074, PPV 0.194 +/- 0.081, and NPV 0.990 +/- 0.011.ConclusionSerial postoperative trajectories improved internal risk stratification for AL, particularly by POD5. The high POD5 NPV should be interpreted in the context of low event prevalence and internal validation; external validation is required before the model is used to support clinical decisions. FAU - Tatar, Ozan Can AU - Tatar OC AUID- ORCID: 0000-0002-9046-7362 AD - Department of General Surgery, Kocaeli City Hospital, Izmit, Kocaeli, Turkey. AD - Faculty of Technology, Department of Biomedical Engineering, Kocaeli University, Izmit, Kocaeli, Turkey. FAU - Ozdamar, Murat AU - Ozdamar M AUID- ORCID: 0009-0007-3852-903X AD - Department of General Surgery, Kocaeli City Hospital, Izmit, Kocaeli, Turkey. FAU - Cepil, Mehmet Fatih AU - Cepil MF AD - Department of General Surgery, Kocaeli City Hospital, Izmit, Kocaeli, Turkey. FAU - Mestci, Ceyhun AU - Mestci C AD - Department of General Surgery, Kocaeli City Hospital, Izmit, Kocaeli, Turkey. FAU - Gunes, Abdullah AU - Gunes A AD - Department of General Surgery, Kocaeli City Hospital, Izmit, Kocaeli, Turkey. FAU - Subasi, Ozkan AU - Subasi O AD - Department of General Surgery, Kocaeli City Hospital, Izmit, Kocaeli, Turkey. FAU - Yazicioglu, Murat Burc AU - Yazicioglu MB AD - Department of General Surgery, Kocaeli City Hospital, Izmit, Kocaeli, Turkey. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Surg Innov JT - Surgical innovation JID - 101233809 SB - IM OTO - NOTNLM OT - C-reactive protein OT - anastomotic leak OT - colorectal surgery OT - dynamic prediction OT - landmark analysis OT - machine learning EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 17:02 PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 17:02 [entrez] AID - 10.1177/15533506261501663 [doi] PST - aheadofprint SO - Surg Innov. 2026 Oct 28:15533506261501663. doi: 10.1177/15533506261501663. PMID- 42950247 OWN - NLM STAT- Publisher LR - 20261028 IS - 2473-9537 (Electronic) IS - 2473-9529 (Linking) DP - 2026 Oct 28 TI - CAR T-cell therapy referral patterns and utilization for relapsed/refractory large B-cell lymphoma in the United States. LID - bloodadvances.2026021289 [pii] LID - 10.1182/bloodadvances.2026021289 [doi] AB - Chimeric antigen receptor (CAR) T-cell therapy has improved outcomes for patients with relapsed/refractory (R/R) large B‑cell lymphoma (LBCL), yet access in routine clinical practice remains limited. We evaluated referral patterns, durability of clinical benefit, and health care resource utilization (HCRU) among US patients with R/R LBCL. Komodo Health US claims data were retrospectively analyzed (October 2016‒May 2025). Adults with R/R LBCL after >/=2 prior lines of therapy who received CAR T-cell therapy at authorized treatment centers (ATC) were included; patients were stratified by referral and treatment status. Outcomes included referral rates, demographics and subsequent treatment, time‑to‑next treatment or death (TTNT-D), and 12‑month postinfusion HCRU. Among 8959 patients, 20% were referred to an ATC and 15% received CAR T-cell therapy (7% originated at an ATC, 8% referred to an ATC). Referred patients were younger (median age, 61.5 vs 67.3 years; P<0.001) and lived closer to ATCs (median distance 17 vs 24 miles; P<0.001). Among referred patients, baseline demographics were similar between CAR T-cell therapy-treated and nontreated groups. In the second‑line closed‑claims cohort, CAR T-cell therapy recipients had longer TTNT-D compared with the nontreated group (median 28 vs 12 months; P<0.005). Inpatient utilization peaked at 1 month postinfusion (mean 1.54 vs 0.62 visits) before declining to similar rates (0.81 vs 0.59). Most eligible patients with R/R LBCL did not receive CAR T-cell therapy due to referral gaps or alternative treatment selection at ATCs. Strengthening referral pathways and increasing awareness of the benefits of CAR T-cell therapy may enable broader, more equitable access. CI - Copyright (c) 2026 American Society of Hematology. FAU - Shadman, Mazyar AU - Shadman M AD - Fred Hutch Cancer Center, Seattle, Washington, United States. FAU - Nastoupil, Loretta J AU - Nastoupil LJ AD - Southwest Oncology, Durango, Colorado, United States. FAU - Patel, Krish AU - Patel K AD - Sarah Cannon Research Institute, Nashville, Tennessee, United States. FAU - Kamdar, Manali AU - Kamdar M AUID- ORCID: 0000-0002-1930-152X AD - University of Colorado Cancer Center, Aurora, Colorado, United States. FAU - Lunning, Matthew A AU - Lunning MA AD - Fred and Pamela Buffett Cancer Center at the University of Nebraska Medical Center, Omaha, Nebraska, United States. FAU - Liu, Fei Fei AU - Liu FF AUID- ORCID: 0000-0003-3364-0808 AD - Bristol Myers Squibb, Princeton, New Jersey, United States. FAU - Fu, Christine T AU - Fu CT AD - Bristol Myers Squibb, Madison, New Jersey, United States. FAU - Cantrell, Michael AU - Cantrell M AD - Bristol Myers Squibb, Princeton, New Jersey, United States. FAU - Goyal, Amit AU - Goyal A AD - ZS Associates, Princeton, New Jersey, United States. FAU - Mukherjee, Dwaipayan AU - Mukherjee D AD - ZS Associates India Pvt. Ltd., Bengaluru, India. FAU - Graff, Tara AU - Graff T AUID- ORCID: 0009-0005-6985-6979 AD - Mission Cancer and Blood, Waukee, Iowa, United States. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Blood Adv JT - Blood advances JID - 101698425 SB - IM EDAT- 2026/10/28 23:27 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 17:02 PHST- 2026/10/13 00:00 [accepted] PHST- 2026/06/02 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 23:27 [pubmed] PHST- 2026/10/28 17:02 [entrez] AID - 571807 [pii] AID - 10.1182/bloodadvances.2026021289 [doi] PST - aheadofprint SO - Blood Adv. 2026 Oct 28:bloodadvances.2026021289. doi: 10.1182/bloodadvances.2026021289. PMID- 42950244 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2561-7605 (Electronic) IS - 2561-7605 (Linking) VI - 9 DP - 2026 Oct 28 TI - Impact of Chatbot-Based Cognitive Training on Cognitive Function and White Matter Integrity in Cognitively Unimpaired Older Adults: Randomized Controlled Trial. PG - e94127 LID - 10.2196/94127 [doi] AB - BACKGROUND: Cognitive training may enhance cognition and support brain health during aging, but few trials have evaluated readily accessible chatbot-based programs with neuroimaging outcomes. OBJECTIVE: This study aimed to evaluate the effects of a chatbot-based cognitive training program on white matter microstructural organization and cognition in cognitively unimpaired older adults. METHODS: In a single-blind randomized controlled trial, 72 cognitively unimpaired older adults were assigned to chatbot-based cognitive training (30 min/day, 5 days/week for 12 weeks; n=37) or a no-intervention control (n=35). Diffusion tensor imaging at baseline and 12 weeks quantified fractional anisotropy (FA) in predefined white matter tracts, and cognition was assessed with the Cambridge Neuropsychological Test Automated Battery. RESULTS: Groups were comparable at baseline. After 12 weeks, the training group showed significantly less FA reduction than controls in the bilateral tapetum and right posterior thalamic radiation, surviving false discovery rate control (P<.05). The training group also improved on Paired Associates Learning (PAL), an episodic memory measure (P=.04). PAL improvement was positively associated with FA change in the right posterior thalamic radiation and right sagittal stratum (false discovery rate-adjusted P=.04 for each). CONCLUSIONS: These findings suggest that a 12-week chatbot-based cognitive training program may support episodic memory and the relative maintenance of white matter microstructural organization in selected pathways in cognitively unimpaired older adults, consistent with a brain-maintenance framework. TRIAL REGISTRATION: CRIS KCT0004630; https://tinyurl.com/4xfesb68. CI - (c)Bori R Kim, Eunhee Kim, Soo Mee Lim, Jinwoo Kim, Jee Hyang Jeong, Geon Ha Kim. Originally published in JMIR Aging (https://aging.jmir.org), 28.10.2026. FAU - Kim, Bori R AU - Kim BR AUID- ORCID: 0000-0002-8069-1213 AD - Department of Neurology, College of Medicine, Ewha Womans University Mokdong Hospital, Ewha Womans University, Seoul, Republic of Korea. AD - Ewha Medical Research Institute, Ewha Womans University, Seoul, Republic of Korea. FAU - Kim, Eunhee AU - Kim E AUID- ORCID: 0000-0003-0085-4615 AD - Department of Radiology, College of Medicine, Ewha Womans University Mokdong Hospital, Ewha Womans University, Seoul, Republic of Korea. FAU - Lim, Soo Mee AU - Lim SM AUID- ORCID: 0000-0003-0782-8583 AD - Department of Neurology, College of Medicine, Ewha Womans University Seoul Hospital, Ewha Womans University, Seoul, Republic of Korea. FAU - Kim, Jinwoo AU - Kim J AUID- ORCID: 0000-0002-0162-5446 AD - Department of Digital Therapeutics Research and Development, HAII Corp., Seoul, Republic of Korea. AD - Graduate School of Cognitive Science, Yonsei University, Seoul, Republic of Korea. FAU - Jeong, Jee Hyang AU - Jeong JH AUID- ORCID: 0000-0001-7945-6956 AD - Department of Neurology, College of Medicine, Ewha Womans University Seoul Hospital, Ewha Womans University, Seoul, Republic of Korea. FAU - Kim, Geon Ha AU - Kim GH AUID- ORCID: 0000-0001-5395-1924 AD - Department of Neurology, College of Medicine, Ewha Womans University Mokdong Hospital, Ewha Womans University, Seoul, Republic of Korea. LA - eng PT - Journal Article PT - Randomized Controlled Trial DEP - 20261028 PL - Canada TA - JMIR Aging JT - JMIR aging JID - 101740387 SB - IM MH - Humans MH - *Cognitive Training/methods MH - *White Matter/diagnostic imaging/physiology MH - Aged MH - *Cognition/physiology MH - Single-Blind Method MH - Female MH - Male MH - Neuropsychological Tests MH - Diffusion Tensor Imaging MH - Cognitive Enhancement MH - Aged, 80 and over OTO - NOTNLM OT - aging OT - chatbot OT - cognitive training OT - diffusion tensor imaging OT - randomized controlled trial OT - white matter EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 16:53 PHST- 2026/03/29 00:00 [received] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/09/23 00:00 [revised] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 16:53 [entrez] AID - v9i1e94127 [pii] AID - 10.2196/94127 [doi] PST - epublish SO - JMIR Aging. 2026 Oct 28;9:e94127. doi: 10.2196/94127. PMID- 42950240 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2369-3762 (Electronic) IS - 2369-3762 (Linking) VI - 12 DP - 2026 Oct 28 TI - Perceived Ratings of AI-Generated and Expert-Reviewed vs Clinician-Developed Virtual Cardiology Patient Scenarios Among Medical Students: Randomized Controlled Study. PG - e100839 LID - 10.2196/100839 [doi] AB - BACKGROUND: Virtual patients (VPs) are widely used in medical education, but creating high-quality cases requires clinical expertise, iterative revision, and alignment with educational objectives. Generative AI may support VP authoring, but direct comparisons between AI-generated and clinician-developed cases delivered within the same educational setting remain limited. OBJECTIVE: This study aimed to compare medical students' learner-perceived ratings of 1 AI-generated, expert-reviewed virtual cardiology patient scenario with those of 1 clinician-developed virtual cardiology patient scenario delivered through the same mobile platform. METHODS: A randomized, participant-blinded 2-arm controlled study was conducted in October 2025 during scheduled fifth-year emergency medicine course sessions. Eligible participants were fifth-year medical students who had completed the cardiology course. Students were assigned 1:1 to complete either a clinician-developed VP (CD-VP; n=64) or a ChatGPT-generated VP (GPT-VP; n=67). Both scenarios followed a shared acute coronary syndrome blueprint and were delivered through the same mobile-optimized app (mobile VPs app [MobiViP]) with identical interface structure and branching logic. The GPT-VP was generated using GPT-5 and refined through iterative follow-up prompting under expert review; direct manual rewriting was prohibited. After completion, students completed a mandatory 19-item questionnaire covering realism and validity, cognitive load, self-reported learning and reflection, and engagement and satisfaction. Analyses included reliability testing, 1-sample tests against a neutral midpoint, Mann-Whitney U tests, and Spearman correlations. Full scenario content and development details are provided as multimedia appendices. RESULTS: A total of 131 complete questionnaires were analyzed, with no item-level missing data. Mean subscale scores in the CD-VP group were 3.81 (SD 0.64) for realism and validity, 3.67 (SD 0.74) for cognitive load, 3.64 (SD 0.73) for self-reported learning and reflection, and 4.16 (SD 0.64) for engagement and satisfaction; corresponding GPT-VP scores were 4.11 (SD 0.56), 3.03 (SD 0.76), 3.86 (SD 0.77), and 4.28 (SD 0.58), respectively. Compared with the CD-VP, the GPT-VP received higher ratings for realism and validity (Mann-Whitney U=1503.0; P=.003) and lower cognitive load ratings, indicating a lower perceived burden (Mann-Whitney U=1070.5; P<.001). Self-reported learning and reflection showed a marginal difference between groups, with a higher rating for the GPT-VP (Mann-Whitney U=1719.0; P=.05), whereas engagement and satisfaction did not differ significantly (Mann-Whitney U=1922.5; P=.31). Internal consistency was acceptable to good for most subscales, although internal consistency for the realism and validity subscale in the GPT-VP group was moderate (Cronbach alpha=0.67). CONCLUSIONS: In this single-center randomized study, 1 AI-generated, expert-reviewed cardiology VP scenario was rated similarly to or more favorably than 1 clinician-developed scenario in selected learner-perceived domains. Future research should expand to objective learning, retention, development workload, and scalability outcomes. CI - (c)Marios Georgios Bantidos, Eleni Dafli, Efstratios Karagiannidis, Barbara Fyntanidou, Sotiria Gilou, Athina Nasoufidou, Panagiotis Bamidis. Originally published in JMIR Medical Education (https://mededu.jmir.org), 28.10.2026. FAU - Bantidos, Marios Georgios AU - Bantidos MG AUID- ORCID: 0009-0001-9409-388X AD - Lab of Medical Physics and Digital Innovation, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Central Macedonia, Greece. AD - Department of Emergency Medicine, AHEPA University General Hospital, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Central Macedonia, Greece. FAU - Dafli, Eleni AU - Dafli E AUID- ORCID: 0000-0002-2439-090X AD - Lab of Medical Physics and Digital Innovation, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Central Macedonia, Greece. FAU - Karagiannidis, Efstratios AU - Karagiannidis E AUID- ORCID: 0000-0001-8328-5942 AD - Department of Emergency Medicine, AHEPA University General Hospital, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Central Macedonia, Greece. FAU - Fyntanidou, Barbara AU - Fyntanidou B AUID- ORCID: 0000-0003-0019-0134 AD - Department of Emergency Medicine, AHEPA University General Hospital, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Central Macedonia, Greece. FAU - Gilou, Sotiria AU - Gilou S AUID- ORCID: 0000-0002-3503-7976 AD - Lab of Medical Physics and Digital Innovation, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Central Macedonia, Greece. FAU - Nasoufidou, Athina AU - Nasoufidou A AUID- ORCID: 0000-0003-3057-5297 AD - Second Department of Cardiology, Hippokration General Hospital, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Central Macedonia, Greece. FAU - Bamidis, Panagiotis AU - Bamidis P AUID- ORCID: 0000-0002-9936-5805 AD - Lab of Medical Physics and Digital Innovation, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Central Macedonia, Greece. LA - eng PT - Journal Article PT - Randomized Controlled Trial DEP - 20261028 PL - Canada TA - JMIR Med Educ JT - JMIR medical education JID - 101684518 SB - IM MH - Humans MH - *Cardiology/education MH - Male MH - *Students, Medical/psychology/statistics & numerical data MH - Female MH - Generative Artificial Intelligence MH - Adult MH - *Patient Simulation MH - Education, Medical, Undergraduate/methods MH - Clinical Competence MH - *Artificial Intelligence MH - Surveys and Questionnaires OTO - NOTNLM OT - ChatGPT OT - RCT OT - VPs OT - cardiology OT - generative AI OT - generative artificial intelligence OT - medical education OT - randomized controlled trial OT - virtual patients EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 23:27 CRDT- 2026/10/28 16:52 PHST- 2026/05/30 00:00 [received] PHST- 2026/08/03 00:00 [accepted] PHST- 2026/07/29 00:00 [revised] PHST- 2026/10/28 23:27 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 16:52 [entrez] AID - v12i1e100839 [pii] AID - 10.2196/100839 [doi] PST - epublish SO - JMIR Med Educ. 2026 Oct 28;12:e100839. doi: 10.2196/100839. PMID- 42950203 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1533-4406 (Electronic) IS - 0028-4793 (Linking) VI - 395 IP - 17 DP - 2026 Oct 29 TI - Gram-Negative Bacterial Resistance. PG - 1728-1743 LID - 10.1056/NEJMra2506114 [doi] AB - The rapid worldwide spread of antimicrobial resistance among gram-negative bacteria is driven by a complex interplay of human, environmental, and health care-associated factors and accelerated by mobile genetic elements that facilitate the dissemination of resistance genes. Resistance in gram-negative bacteria arises primarily through reduced drug entry, active drug efflux, modification of antibiotic targets, and enzymatic drug inactivation, most notably through beta-lactamase production. Clinically important resistant pathogens include extended-spectrum beta-lactamase-producing Enterobacterales, carbapenem-resistant Enterobacterales, Pseudomonas aeruginosa with difficult-to-treat resistance, and carbapenem-resistant Acinetobacter baumannii. Limited access to effective antibiotics and the continued emergence of resistance to available agents pose substantial challenges to the management of these infections. Appropriate management requires timely and accurate diagnostics, targeted use of newer beta-lactam agents, effective source control, and judicious selection of treatment durations. CI - Copyright (c) 2026 Massachusetts Medical Society. FAU - Tamma, Pranita D AU - Tamma PD AUID- ORCID: 0000-0002-4143-6324 AD - University of Pennsylvania Perelman School of Medicine, Philadelphia. AD - Children's Hospital of Philadelphia, Philadelphia. LA - eng PT - Journal Article PT - Review PL - United States TA - N Engl J Med JT - The New England journal of medicine JID - 0255562 RN - 0 (Anti-Bacterial Agents) RN - 0 (beta Lactam Antibiotics) RN - EC 3.5.2.6 (beta-Lactamases) RN - 0 (Carbapenems) RN - 0 (Bacterial Proteins) RN - 0 (Porins) RN - EC 3.5.2.6 (carbapenemase) SB - IM MH - Humans MH - *Anti-Bacterial Agents/therapeutic use/pharmacology MH - beta Lactam Antibiotics/pharmacology/therapeutic use MH - beta-Lactam Resistance/genetics MH - beta-Lactamases/genetics/metabolism MH - Carbapenems/pharmacology/therapeutic use MH - *Drug Resistance, Bacterial/genetics MH - *Drug Resistance, Multiple, Bacterial/genetics MH - *Gram-Negative Bacteria/drug effects/enzymology/genetics/metabolism MH - *Gram-Negative Bacterial Infections/diagnosis/drug therapy/microbiology MH - Interspersed Repetitive Sequences/genetics MH - Bacterial Proteins/genetics/metabolism MH - Metabolic Reprogramming/genetics MH - Porins/genetics/metabolism MH - Bacterial Outer Membrane/metabolism MH - Drug Therapy, Combination/methods MH - Antimicrobial Stewardship EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 CRDT- 2026/10/28 16:32 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 16:32 [entrez] AID - 10.1056/NEJMra2506114 [doi] PST - ppublish SO - N Engl J Med. 2026 Oct 29;395(17):1728-1743. doi: 10.1056/NEJMra2506114. PMID- 42950179 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2687-8941 (Electronic) IS - 2687-8941 (Linking) VI - 12 IP - 10 DP - 2026 Oct TI - Socioeconomic and Systemic-Level Determinants of Stage at Diagnosis in Colon and Rectal Cancer: A 30-Year Population-Based Study in Quito, Ecuador. PG - e2600271 LID - 10.1200/GO-26-00271 [doi] AB - PURPOSE: Health systems in middle-income countries are often compromised by parallel, unintegrated subsystems (public safety-net, social security, private care), exacerbating health disparities. We evaluated how this architecture and individual socioeconomic position relate to advanced clinical stage at diagnosis, stratified by anatomic site (colon and rectal) and age cohort, in Quito, Ecuador, over a 30-year period (1990-2019). METHODS: After excluding unstaged and death certificate-only cases, data from 2,552 patients were analyzed. Selection bias was evaluated using Cramer's V and rank-biserial effect sizes. Multivariable logistic regression identified factors associated with synchronous late-stage (stage IV) presentation, adjusting for demographics, socioeconomic markers, and diagnostic health sector. Secondary overall survival trends were evaluated using Kaplan-Meier estimates and exploratory stage-stratified Cox models. RESULTS: In patients >/=65 years, public-sector diagnosis was associated with a higher odds of late-stage colon cancer (adjusted Odds Ratio = 2.04; 95% CI, 1.24 to 3.35) compared with diagnosis in the private sector but showed no association with rectal cancer. Late-stage diagnosis probability remained stable over the 30-year study period. Exploratory survival models linked public-sector entry to higher mortality hazards (adjusted hazard ratios, 1.35-3.01), with adjusted survival trajectories exhibiting broad temporal stability across recent decades. CONCLUSION: Public-sector care s persistently associated with diagnostic delays that has spanned three decades, independent of socioeconomic position. Beyond early-detection barriers, exploratory survival models generate the hypothesis that public-sector disparities extend into longitudinal survival outcomes, reinforcing the urgent need for structural reforms such as organized screening and equitable public care pathways. FAU - Albacura-Campues, Carol T AU - Albacura-Campues CT AUID- ORCID: 0009-0002-8987-8540 AD - Universidad Internacional del Ecuador UIDE, Quito, Ecuador. FAU - Ayala Davila, Raul A AU - Ayala Davila RA AUID- ORCID: 0000-0001-8000-6274 AD - Universidad Internacional del Ecuador UIDE, Quito, Ecuador. AD - Grups de Recerca d'America i Africa Llatines/GRAAL, Barcelona, Spain. FAU - Gualan, Monsermin AU - Gualan M AUID- ORCID: 0000-0002-5989-3187 AD - Universidad Internacional del Ecuador UIDE, Quito, Ecuador. AD - Grups de Recerca d'America i Africa Llatines/GRAAL, Barcelona, Spain. FAU - Vera, David AU - Vera D AUID- ORCID: 0000-0001-6922-5687 AD - Universidad Internacional del Ecuador UIDE, Quito, Ecuador. FAU - Cooper, Philip J AU - Cooper PJ AUID- ORCID: 0000-0002-6770-6871 AD - Universidad Internacional del Ecuador UIDE, Quito, Ecuador. AD - School of Health and Medical Sciences, City St George's University of London, London, UK. FAU - Romero-Sandoval, Natalia AU - Romero-Sandoval N AUID- ORCID: 0000-0001-6881-6581 AD - Universidad Internacional del Ecuador UIDE, Quito, Ecuador. AD - Grups de Recerca d'America i Africa Llatines/GRAAL, Barcelona, Spain. CN - SEDHI UNIT LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - JCO Glob Oncol JT - JCO global oncology JID - 101760170 SB - IM MH - Humans MH - Ecuador/epidemiology MH - Female MH - Male MH - Aged MH - Neoplasm Staging MH - *Colonic Neoplasms/diagnosis/epidemiology/pathology/mortality MH - Middle Aged MH - *Rectal Neoplasms/diagnosis/epidemiology/pathology/mortality MH - Socioeconomic Disparities in Health MH - Socioeconomic Factors EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 CRDT- 2026/10/28 16:03 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 16:03 [entrez] AID - 10.1200/GO-26-00271 [doi] PST - ppublish SO - JCO Glob Oncol. 2026 Oct;12(10):e2600271. doi: 10.1200/GO-26-00271. Epub 2026 Oct 28. PMID- 42950176 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2687-8941 (Electronic) IS - 2687-8941 (Linking) VI - 12 IP - 10 DP - 2026 Oct TI - Framework for Health Technology Assessment for Universal Cancer Care in India. PG - e2600071 LID - 10.1200/GO-26-00071 [doi] AB - India faces a growing cancer burden, with late diagnosis, high treatment costs, and out-of-pocket expenditure posing challenges to achieving universal cancer care (UCC). This paper examines the role of health technology assessment (HTA) in informing priority setting, strategic purchasing, and resource allocation for cancer care in India, and discusses its contribution toward achieving UCC. We undertook a targeted review of published literature and policy documents describing India's UCC initiatives and institutionalization of HTA. Bibliographic databases were searched to identify HTA and economic evaluation studies in oncology conducted under the aegis of Health Technology Assessment in India (HTAIn), the central HTA agency of the Government of India. The HTAIn website was additionally reviewed to identify completed oncology HTA reports. Evidence was synthesized narratively to describe the applications of HTA and key challenges in translating HTA evidence into policy and practice. HTA has informed multiple dimensions of publicly financed cancer care in India, including selection of interventions for health benefit packages under Ayushman Bharat Pradhan Mantri Jan Aarogya Yojana, development and refinement of treatment and program guidelines, and generation of value-based prices to inform pricing and reimbursement decisions. Applications span cancer prevention and screening, pharmaceuticals, radiotherapy, and other treatment modalities. HTA also provides a framework for considering efficiency, equity, financial risk protection, and health-system implications when allocating limited resources. HTA can play a pivotal role in advancing UCC in India by supporting transparent, evidence-informed priority setting and strategic purchasing. Strengthening mechanisms for timely HTA, evidence uptake, periodic reassessment, and linkage with reimbursement and pricing decisions are important to maximizing health gains, promoting equity, and reducing financial hardship. FAU - Gupta, Nidhi AU - Gupta N AUID- ORCID: 0000-0003-4079-2631 AD - Department of Radiation Oncology, Government Medical College and Hospital, Chandigarh, India. FAU - Jyani, Gaurav AU - Jyani G AUID- ORCID: 0000-0003-3787-2446 AD - Department of Community Medicine and School of Public Health, Post Graduate Institute of Medical Education and Research, Chandigarh, India. FAU - Prinja, Shankar AU - Prinja S AUID- ORCID: 0000-0001-7719-6986 AD - Department of Community Medicine and School of Public Health, Post Graduate Institute of Medical Education and Research, Chandigarh, India. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - JCO Glob Oncol JT - JCO global oncology JID - 101760170 SB - IM MH - Humans MH - India MH - *Technology Assessment, Biomedical/methods MH - *Neoplasms/therapy/economics/epidemiology MH - *Universal Health Care MH - Cost-Benefit Analysis EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 CRDT- 2026/10/28 16:03 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 16:03 [entrez] AID - 10.1200/GO-26-00071 [doi] PST - ppublish SO - JCO Glob Oncol. 2026 Oct;12(10):e2600071. doi: 10.1200/GO-26-00071. Epub 2026 Oct 28. PMID- 42950173 OWN - NLM STAT- Publisher LR - 20261028 IS - 1527-7755 (Electronic) IS - 0732-183X (Linking) DP - 2026 Oct 28 TI - KEYNOTE-867: A Phase III, Randomized, Double-Blind Study of Stereotactic Body Radiotherapy With Pembrolizumab for Unresected Stage I or II Non-Small Cell Lung Cancer. PG - JCO2600404 LID - 10.1200/JCO-26-00404 [doi] AB - PURPOSE: Stereotactic body radiotherapy (SBRT) is standard of care for inoperable stage I or II non-small cell lung cancer (NSCLC). KEYNOTE-867 (ClinicalTrials.gov identifier: NCT03924869) evaluated the addition of pembrolizumab to SBRT in participants diagnosed with stage I or II NSCLC that was inoperable or in participants who refused surgery. METHODS: Adults with untreated, stage I or II (T1 to limited T3, N0, M0) NSCLC (AJCC 8(th) edition) that was inoperable, or adults who refused surgery, were randomly assigned 1:1 to thoracic SBRT to primary tumors for /=1 dose of study treatment, grade 3-5 treatment-related adverse events (AEs) occurred in 46/226 (20.4%) participants in the pembrolizumab arm and 8/218 (3.7%) participants in the placebo arm. There were five treatment-related deaths all of which occurred in the pembrolizumab arm (2.2%). CONCLUSION: The addition of pembrolizumab to SBRT did not improve EFS and was associated with a higher incidence of treatment-related AEs compared with SBRT alone in participants with inoperable stage I or II NSCLC. Based on the benefit-risk profile, the KEYNOTE-867 trial was prematurely stopped, and all study treatment was discontinued. FAU - Jabbour, Salma K AU - Jabbour SK AUID- ORCID: 0000-0001-8200-5371 AD - Department of Radiation Oncology, Rutgers Cancer Institute, Rutgers University, New Brunswick, NJ. FAU - Soter, Szabolcs AU - Soter S AD - Department of Pulmonology, National Koranyi Institute of Pulmonology, Budapest, Hungary. FAU - Eaton, Michael AU - Eaton M AD - Radiation Therapy Department, Franciscan Health, Indianapolis, IN. FAU - Galffy, Gabriella AU - Galffy G AUID- ORCID: 0000-0001-7255-035X AD - Pulmonology Center of the Reformed Church in Hungary, Torokbalint, Hungary. FAU - Kowalski, Dariusz AU - Kowalski D AUID- ORCID: 0000-0002-9452-3229 AD - Department of Lung Cancer and Thoracic Tumors, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland. FAU - Hata, Akito AU - Hata A AUID- ORCID: 0000-0001-7035-7249 AD - Division of Thoracic Oncology, Kobe Minimally Invasive Cancer Center, Kobe, Japan. FAU - Valdivia, Augusto AU - Valdivia A AUID- ORCID: 0000-0002-8039-0007 AD - Medical Oncology Department, Vall d'Hebron Institute of Oncology (VHIO)-Vall d'Hebron Hospital Universitari, Barcelona, Spain. FAU - Frost, Nikolaj AU - Frost N AUID- ORCID: 0000-0001-7452-7129 AD - Department of Infectious Diseases and Respiratory Medicine, Charite-Universitatsmedizin Berlin, Berlin, Germany. FAU - Levchenko, Evgeny AU - Levchenko E AUID- ORCID: 0000-0003-3837-2515 AD - Department of Thoracic Oncology, N. N. Petrov National Medical Research Center of Oncology, Sankt-Petersburg, Russia. FAU - Monteiro, Mariana AU - Monteiro M AUID- ORCID: 0000-0001-9552-6977 AD - Department of Medical Oncology, Americas Institute, Sao Paulo, Brazil. FAU - Haridass, Anoop AU - Haridass A AUID- ORCID: 0000-0002-4185-290X AD - Radiation Oncology Division, The Clatterbridge Cancer Centre, Liverpool, United Kingdom. FAU - Bouchard, Myriam AU - Bouchard M AD - Service de radiooncologie, Centre Integre Universitaire de Sante et de Services Sociaux de l'Estrie-Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, Quebec, Canada. FAU - Lee, Ki Hyeong AU - Lee KH AUID- ORCID: 0000-0002-7830-5950 AD - Department of Internal Medicine, Chungbuk National University Hospital, Cheongju, South Korea. FAU - Ahn, Myung-Ju AU - Ahn MJ AUID- ORCID: 0000-0002-5740-9654 AD - Division of Hematology-Oncology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea. FAU - Hughes, Brett G M AU - Hughes BGM AUID- ORCID: 0000-0002-1811-0537 AD - Department of Cancer Care Services, Royal Brisbane and Women's Hospital and The University of Queensland, Brisbane, Australia. FAU - Durham, Andre AU - Durham A AUID- ORCID: 0000-0002-5875-0301 AD - Service de radio-oncologie, Hopitaux Universitaires de Geneve, Geneva, Switzerland. FAU - Xu, Yan AU - Xu Y AD - Biostatistics and Research Decision Sciences, Merck & Co, Inc, Rahway, NJ. FAU - Samkari, Ayman AU - Samkari A AD - Global Clinical Development, Merck & Co, Inc, Rahway, NJ. FAU - Song, Andrew AU - Song A AUID- ORCID: 0000-0001-8386-4397 AD - Global Clinical Development, Merck & Co, Inc, Rahway, NJ. FAU - Pircher, Andreas AU - Pircher A AUID- ORCID: 0000-0002-7747-3012 AD - Internal Medicine V, Department of Hematology and Oncology, Medical University of Innsbruck, Innsbruck, Austria. LA - eng SI - ClinicalTrials.gov/NCT03924869 PT - Journal Article DEP - 20261028 PL - United States TA - J Clin Oncol JT - Journal of clinical oncology : official journal of the American Society of Clinical Oncology JID - 8309333 SB - IM EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 16:02 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 16:02 [entrez] AID - 10.1200/JCO-26-00404 [doi] PST - aheadofprint SO - J Clin Oncol. 2026 Oct 28:JCO2600404. doi: 10.1200/JCO-26-00404. PMID- 42950166 OWN - NLM STAT- Publisher LR - 20261028 IS - 1545-4487 (Electronic) IS - 0066-4170 (Linking) DP - 2026 Oct 28 TI - Chemical Ecology and Management of Imported Fire Ants. LID - 10.1146/annurev-ento-012826-052349 [doi] AB - Imported fire ants (Solenopsis invicta and S. richteri) are invasive pests that were introduced into the United States via Mobile, Alabama, in the early twentieth century. As these species spread, interbreeding led to the emergence of hybrid populations. Their rapid expansion and ecological dominance prompted extensive control efforts, including chemical eradication campaigns and biologically informed strategies. Today, fire ants infest over 140 million hectares across the southern United States, affecting public health, agriculture, and ecosystems. Despite decades of research, many fire ant semiochemicals remain unidentified because of the ants' complex social behavior and because multicomponent chemical signals occur in trace amounts and are difficult to isolate, especially those involved in aerial mating. Additionally, components often lack activity unless presented in specific combinations. These challenges limit traditional bioassay-guided structure elucidation. This review summarizes advances in fire ant chemical ecology, highlights persistent knowledge gaps, and proposes innovative strategies to accelerate pheromone discovery and improve management. FAU - Chen, Jian AU - Chen J AD - Agricultural Research Service, Southeast Area, Biological Control of Pests Research Unit, United States Department of Agriculture, Stoneville, Mississippi, USA; email: jian.chen@usda.gov. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Annu Rev Entomol JT - Annual review of entomology JID - 0372367 SB - IM EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 15:52 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 15:52 [entrez] AID - 10.1146/annurev-ento-012826-052349 [doi] PST - aheadofprint SO - Annu Rev Entomol. 2026 Oct 28. doi: 10.1146/annurev-ento-012826-052349. PMID- 42950163 OWN - NLM STAT- Publisher LR - 20261028 IS - 1744-5086 (Electronic) IS - 0928-6586 (Linking) DP - 2026 Oct 28 TI - Glaucoma Treatment Trends in Australia Over a 10-Year Period. PG - 1-8 LID - 10.1080/09286586.2026.2754994 [doi] AB - PURPOSE: To characterise trends in the use of selective laser trabeculoplasty (SLT), glaucoma surgery and medication prescribing in Australia between 2015 and 2024. STUDY DESIGN: Nationwide, population-based cross-sectional analysis. METHODS: Medicare Benefits Schedule (MBS) procedure claims and Pharmaceutical Benefits Scheme (PBS)/Repatriation PBS dispensing data were analysed from 2015 to 2024. Procedure and prescription rates were expressed per 100,000 Australian residents using Australian Bureau of Statistics population estimates. RESULTS: Between 2015 and 2024, 344,370 SLT procedures were recorded. Rates rose from 127 to 150 per 100,000 (beta = 1,335 procedures/year; 95% CI 969-1,701; p < 0.001). Annual glaucoma medication prescriptions decreased from 4,167,417 to 3,390,394 (17,498 to 12,466 per 100,000), without a statistically significant linear trend (beta = -52,005/year; 95% CI - 104,373-363; p = 0.051). Overall, 113,466 glaucoma surgeries were recorded: annual procedures increased from 4,953 (20.8 per 100,000) to 15,334 (56.4 per 100,000). Minimally invasive glaucoma surgeries (MIGS) increased from 2,177 in 2017 to 10,501 in 2024, compared with 4,080 to 4,386 filtration procedures and 292 to 409 drainage device procedures. Negative binomial regression found no significant annual rate trends for MIGS (IRR 1.16; 95% CI 0.86-1.57; p = 0.34), filtration procedures (IRR 1.00; 95% CI 0.81-1.24; p = 0.97), or drainage devices (IRR 1.03; 95% CI 0.83-1.28; p = 0.79). Optometrists' share of glaucoma prescriptions increased from 1.25% to 5.63%. CONCLUSIONS: Our findings demonstrate changing glaucoma treatment trends in Australia. SLT and MIGS procedure volumes increased as medication prescriptions declined, while optometrists accounted for an increasing proportion of medication prescribing. FAU - Rana, Khizar AU - Rana K AD - Department of Ophthalmology & Visual Sciences, University of Adelaide, Adelaide, SA, Australia. AD - Department of Ophthalmology, Queen Elizabeth Hospital, Woodville South, SA, Australia. FAU - Paris, Jai AU - Paris J AD - Department of Ophthalmology & Visual Sciences, University of Adelaide, Adelaide, SA, Australia. FAU - Chan, Weng Onn AU - Chan WO AD - Department of Ophthalmology & Visual Sciences, University of Adelaide, Adelaide, SA, Australia. AD - Department of Ophthalmology, Queen Elizabeth Hospital, Woodville South, SA, Australia. FAU - Sun, Michelle T AU - Sun MT AD - Department of Ophthalmology & Visual Sciences, University of Adelaide, Adelaide, SA, Australia. FAU - Casson, Robert AU - Casson R AD - Department of Ophthalmology & Visual Sciences, University of Adelaide, Adelaide, SA, Australia. FAU - Cheng, Jason AU - Cheng J AD - Sydney Medical School, Faculty of Medicine and Health, The University of Sydney, Sydney, Australia. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Ophthalmic Epidemiol JT - Ophthalmic epidemiology JID - 9435674 SB - IM OTO - NOTNLM OT - Glaucoma OT - Pharmaceutical Benefits Scheme OT - glaucoma therapy OT - selective laser trabeculoplasty OT - treatment EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 15:32 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 15:32 [entrez] AID - 10.1080/09286586.2026.2754994 [doi] PST - aheadofprint SO - Ophthalmic Epidemiol. 2026 Oct 28:1-8. doi: 10.1080/09286586.2026.2754994. PMID- 42950153 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1532-6586 (Electronic) IS - 0161-4754 (Linking) VI - 49 IP - 1 DP - 2026 Sep 25 TI - Effect of applied spinal manipulation force direction on vertebral accelerations: An in vivo ovine study. LID - eJMPT-D-26-00008 [pii] LID - 10.67400/JMPT-D-26-00008 [doi] AB - OBJECTIVE: The purpose of this study was to determine the effect of varying spinal manipulative thrust (SMT) applied force angle on vertebral accelerations in an in vivo ovine model. METHODS: Twelve healthy young Merino sheep (mean 24 kg, SD 3 kg) were tested under institutional ethics approval. Tri-axial accelerometers rigidly attached to intraosseous pins at L2 and L4 spinous processes quantified vertebral accelerations during SMTs delivered to L3 at 3 force vectors: 90 degrees (posteroanterior), 60 degrees (cephalad), and 120 degrees (caudal). Thirteen SMTs ( approximately 200 N peak force) were applied at approximately 6 Hz per condition across 3 randomized repeated-measures trials. Peak-to-peak (p-p) accelerations were compared among force directions using 1-way analysis of variance. RESULTS: Significantly increased p-p accelerations were observed for caudally directed (120 degrees ) thrusts at both L2 and L4 (P < .01), as well as at the L2-L4 segmental transfer level. Cephalad thrusts (60 degrees ) produced greater L2 than L4 accelerations; caudal thrusts (120 degrees ) produced greater L4 than L2 accelerations. CONCLUSION: The applied force direction significantly influences segmental and adjacent-segment vertebral accelerations, with implications for optimizing line-of-drive selection in clinical SMT practice. FAU - Colloca, Christopher J AU - Colloca CJ AUID- ORCID: 0000-0002-6381-8278 FAU - Kosmopoulos, Victor AU - Kosmopoulos V AUID- ORCID: 0000-0002-9238-8414 FAU - Gunzburg, Robert AU - Gunzburg R AUID- ORCID: 0000-0002-5882-0664 LA - eng PT - Journal Article DEP - 20260925 PL - United States TA - J Manipulative Physiol Ther JT - Journal of manipulative and physiological therapeutics JID - 7807107 SB - IM MH - Animals MH - *Manipulation, Spinal/methods MH - Sheep MH - *Acceleration MH - Biomechanical Phenomena MH - *Lumbar Vertebrae/physiology MH - Female OTO - NOTNLM OT - Acceleration OT - Biomechanics OT - Lumbar OT - Manipulation OT - Ovine Model OT - Spinal OT - Vertebrae EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 CRDT- 2026/10/28 15:12 PHST- 2026/03/22 00:00 [received] PHST- 2026/05/03 00:00 [accepted] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 15:12 [entrez] AID - eJMPT-D-26-00008 [pii] AID - 10.67400/JMPT-D-26-00008 [doi] PST - epublish SO - J Manipulative Physiol Ther. 2026 Sep 25;49(1):eJMPT-D-26-00008. doi: 10.67400/JMPT-D-26-00008. Print 2026 Sep 25. PMID- 42950148 OWN - NLM STAT- Publisher LR - 20261028 IS - 1944-8252 (Electronic) IS - 1944-8244 (Linking) DP - 2026 Oct 29 TI - First Evidence and Role of gamma-Fe2O3 Seeds in Low-Temperature-Processed Sn-Doped Hematite Photoanodes. LID - 10.1021/acsami.6c15012 [doi] AB - Sn doping is an effective strategy to improve the photoelectrochemical response of hematite photoanodes, but its activation typically requires high-temperature treatments that can degrade conductive substrates and limit device integration. Considering photoelectrochemical water splitting as a case study, this work investigates the mechanism of photoelectrochemical activation of Sn-doped hematite-based photoanodes by oxidative annealing of thin metallic Fe-Sn alloy films at a low temperature. X-ray absorption spectroscopy, Raman spectroscopy, grazing-incidence X-ray diffraction, and electron paramagnetic resonance reveal that while hematite is the main crystalline phase, poorly ordered gamma-Fe2O3 seeds selectively form in Sn-containing films annealed at 450 degrees C and progressively transform into Sn-doped alpha-Fe2O3 at higher annealing temperatures. Under solar illumination in 1 M KOH, photoanodes annealed at 450 degrees C exhibit photocurrent density ( approximately 480 muA/cm2) and onset potential ( approximately 0.9 V vs RHE) comparable to those obtained after annealing at a higher temperature (750 degrees C). Sn K-edge X-ray absorption spectroscopy identifies Sn4+ in local Sn-Fe coordination environments consistent with incorporation into the hematite lattice. The gamma-Fe2O3 domains may act as transient Sn reservoirs, accommodating Sn beyond the hematite solubility limit and promoting the formation of well-ordered, photoactive Sn-doped alpha-Fe2O3 nanocrystals, while limiting SnO2 segregation at an Sn/(Fe + Sn) atomic ratio of approximately 3 at %. Control films deposited under an oxygen atmosphere contain a lower gamma-Fe2O3 fraction and show poor activity, indicating that low-temperature activation arises not from Sn addition alone but requires oxidation-driven restructuring of the metallic Fe-Sn precursor. The gamma-Fe2O3 seeds represent structural fingerprints of a synthesis pathway that enables efficient Sn incorporation and low-thermal-budget fabrication of hematite photoanodes for solar water splitting. CI - (c) 2026 The Author(s). Published by American Chemical Society. FAU - Ragonese, P AU - Ragonese P AD - Department of Physics and Astronomy, University of Padova, Padova35131, Italy. FAU - Kalinic, B AU - Kalinic B AUID- ORCID: 0000-0003-1750-4929 AD - Department of Physics and Astronomy, University of Padova, Padova35131, Italy. FAU - Mattioli, G AU - Mattioli G AD - Department of Physics and Astronomy, University of Padova, Padova35131, Italy. FAU - Naveenkumar, N W AU - Naveenkumar NW AD - Department of Physics and Astronomy, University of Padova, Padova35131, Italy. FAU - Franco, L AU - Franco L AUID- ORCID: 0000-0003-3548-4423 AD - Department of Chemical Sciences, University of Padova, Padova35131, Italy. FAU - Mattei, G AU - Mattei G AD - Department of Physics and Astronomy, University of Padova, Padova35131, Italy. FAU - Maurizio, C AU - Maurizio C AUID- ORCID: 0000-0002-0517-1314 AD - Department of Physics and Astronomy, University of Padova, Padova35131, Italy. LA - eng GR - 202277JCZ5/Ministero dell'Universita e della Ricerca/ GR - C93C22002750006/Ministero dell'Universita e della Ricerca/ GR - CN00000023/Ministero dell'Universita e della Ricerca/ PT - Journal Article DEP - 20261029 PL - United States TA - ACS Appl Mater Interfaces JT - ACS applied materials & interfaces JID - 101504991 SB - IM OTO - NOTNLM OT - Sn-doping OT - hematite photoanodes OT - physical vapor deposition OT - water splitting OT - gamma-Fe3O3 EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 15:03 PHST- 2026/07/21 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 15:03 [entrez] AID - 5460439 [pii] AID - 10.1021/acsami.6c15012 [doi] PST - aheadofprint SO - ACS Appl Mater Interfaces. 2026 Oct 29. doi: 10.1021/acsami.6c15012. PMID- 42950147 OWN - NLM STAT- Publisher LR - 20261028 IS - 2998-8756 (Electronic) IS - 2998-8748 (Linking) DP - 2026 Oct 28 TI - Occupational Stressors, Perceived Injustice, and Pathways to Pain Interference and Insomnia Among Workers With Headache or Low Back Pain. LID - 10.1097/PSY.0000000000001543 [doi] AB - OBJECTIVE: Perceived injustice is a psychosocial risk factor associated with delayed recovery and poor occupational outcomes among workers with pain conditions. We examined a theory-informed pathway linking occupational stressors, perceived injustice, pain interference, depressive symptoms, and insomnia, while evaluating selected measurement properties of the Japanese Injustice Experience Questionnaire-Short Form (IEQ-SF-J). METHODS: This cross-sectional online survey included 600 Japanese workers (300 with headache and 300 with low back pain). Structural validity of the IEQ-SF-J was evaluated using exploratory and confirmatory factor analyses (CFA) and item response theory. Construct validity was examined using Spearman correlations. Structural equation modelling (SEM) estimated indirect associations between job demands or low job control and pain interference, depressive symptoms, and insomnia, with perceived injustice specified as a latent intervening construct. RESULTS: CFA supported a unidimensional structure (comparative fit index=0.993; root mean square error of approximation=0.080). Internal consistency was acceptable (Cronbach's alpha=0.82; McDonald's omega=0.90), and test-retest reliability was moderate (intraclass correlation coefficient=0.60). IEQ-SF-J scores correlated significantly with pain interference, insomnia, and depressive symptoms. Job demands and low job control showed significant indirect associations with all three outcomes involving perceived injustice. CONCLUSIONS: The IEQ-SF-J showed supportive evidence for several measurement properties and occupied a theoretically coherent position in associations linking occupational stressors with pain interference, depressive symptoms, and insomnia among workers with headache or low back pain. CI - Copyright (c) 2026 Society for Biopsychosocial Science and Medicine. FAU - Yamada, Keiko AU - Yamada K AUID- ORCID: 0000-0002-4378-3290 FAU - Mibu, Akira AU - Mibu A AUID- ORCID: 0000-0002-6141-7809 FAU - Adachi, Tomonori AU - Adachi T AUID- ORCID: 0000-0002-6227-0802 FAU - Enomoto, Kiyoka AU - Enomoto K AUID- ORCID: 0000-0002-3193-8259 FAU - Nishigami, Tomohiko AU - Nishigami T AUID- ORCID: 0000-0002-6969-8916 FAU - Sullivan, Michael AU - Sullivan M AUID- ORCID: 0000-0002-4228-1678 LA - eng GR - JP21K18100; JP24K21147/Japan Society for Promotion of Society/ PT - Journal Article DEP - 20261028 PL - United States TA - Biopsychosoc Sci Med JT - Biopsychosocial science and medicine JID - 9918976361906676 SB - IM OTO - NOTNLM OT - 10th Revision OT - 5-item version OT - AIS-5 = Athens Insomnia Scale OT - CFA = confirmatory factor analysis OT - CFI = comparative fit index OT - CI = confidence interval OT - DC-JBAP2020 = 2020 Japanese Biopsychosocial Assessment of Pain project OT - DIF = differential item functioning OT - EFA = exploratory factor analysis OT - ICC = intraclass correlation coefficient OT - ICD-10 = International Classification of Diseases OT - IEQ = Injustice Experience Questionnaire OT - IEQ-SF = Injustice Experience Questionnaire-Short Form OT - IEQ-SF-J = Japanese version of the Injustice Experience Questionnaire-Short Form OT - IRT = item response theory OT - ISPOR = International Society for Pharmacoeconomics and Outcomes Research OT - Japan OT - NRS = Numeric Rating Scale OT - PCS = Pain Catastrophizing Scale OT - PDI = Pain Disability Index OT - PDI-5 = 5-item Pain Disability Index scoring format OT - PHQ-2 = Patient Health Questionnaire-2 OT - RMSEA = root mean square error of approximation OT - SEM = structural equation modelling OT - SRMR = standardized root mean square residual OT - TLI = Tucker-Lewis index OT - WLSMV = weighted least squares mean and variance adjusted. OT - chronic pain OT - measurement properties OT - occupational health OT - perceived injustice OT - structural equation modelling EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 15:03 PHST- 2026/05/04 00:00 [received] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 15:03 [entrez] AID - 02276378-990000000-00165 [pii] AID - 10.1097/PSY.0000000000001543 [doi] PST - aheadofprint SO - Biopsychosoc Sci Med. 2026 Oct 28. doi: 10.1097/PSY.0000000000001543. PMID- 42950144 OWN - NLM STAT- Publisher LR - 20261028 IS - 2373-9878 (Electronic) IS - 2373-9878 (Linking) DP - 2026 Oct 28 TI - 3D-Printed Microneedles for Periodontal and Oral Mucosal Therapy: A Systematic Review of Therapeutic and Fabrication Strategies. LID - 10.1021/acsbiomaterials.6c01471 [doi] AB - Microneedles (MNs) technologies have emerged as a promising platform for localized drug delivery in the mouth, offering minimally invasive access to the oral mucosa, including gingival tissues. However, their integration with advanced additive manufacturing remains underexplored, despite the need for high-precision, patient-specific devices that can overcome the limitations of conventional pharmacotherapy. This review analyzes the recent developments in 3D-printed MNs for intraoral drug delivery, focusing on polymeric designs enabled by stereolithography, digital light processing, continuous liquid interface production, and two-photon polymerization. High-resolution vat-photopolymerization systems demonstrate superior spatial resolution and fabrication accuracy, allowing the reproducible production of MNs geometries with optimized tip radii, internal channels, and drug-loading microstructures. MNs-mediated delivery of antimicrobial, anti-inflammatory, and healing-promoting agents demonstrates potential not only for the management of periodontal inflammation, oral mucosal lesions, and soft-tissue defects but also for preventive interventions in early-stage diseases and for the control of recurrence. Despite these advances, major barriers persist, including limited in vivo validation under dynamic oral conditions, insufficient mechanical assessment under masticatory forces and salivary flow, and a lack of standardized regulatory frameworks for intraoral MNs devices. Overall, the current findings indicate that 3D-printed MNs represent an emerging and highly promising class of localized therapeutic delivery systems for the management of conditions affecting the periodontal tissues and the oral mucosa, with substantial opportunities for technological refinement and clinical translation. CI - (c) 2026 The Author(s). Published by American Chemical Society. FAU - Pereira, Luis M AU - Pereira LM AD - CICECO, Aveiro Institute of Materials, University of Aveiro , Campus Universitario de Santiago, Aveiro3810-193, Portugal. AD - TEMA - Centre for Mechanical Technology and Automation, Department of Mechanical Engineering, University of Aveiro, Aveiro3810-193, Portugal. FAU - Lei, Catarina Costa AU - Lei CC AD - CICECO, Aveiro Institute of Materials, University of Aveiro , Campus Universitario de Santiago, Aveiro3810-193, Portugal. AD - TEMA - Centre for Mechanical Technology and Automation, Department of Mechanical Engineering, University of Aveiro, Aveiro3810-193, Portugal. FAU - Herrera, David AU - Herrera D AD - ETEP (Etiology and Therapy of Periodontal and Peri-Implant Diseases) Research Group, University Complutense, Madrid28040, Spain. FAU - Oliveira, Jose M AU - Oliveira JM AUID- ORCID: 0000-0003-1564-5728 AD - CICECO, Aveiro Institute of Materials, University of Aveiro , Campus Universitario de Santiago, Aveiro3810-193, Portugal. AD - School of Design, Management and Production Technologies Northern Aveiro, Estrada do Cercal 449, Santiago da Riba-Ul3720-511, Portugal. FAU - Mesquita-Guimaraes, Joana AU - Mesquita-Guimaraes J AUID- ORCID: 0000-0002-6342-7824 AD - TEMA - Centre for Mechanical Technology and Automation, Department of Mechanical Engineering, University of Aveiro, Aveiro3810-193, Portugal. AD - LASI - Intelligent Systems Associate Laboratory, Guimaraes4800-058, Portugal. LA - eng GR - 2025.09118.CBM/German Academic Exchange Service/ GR - 2024.00977.BD/Fundacao para a Ciencia e a Tecnologia/ GR - 2025.09118.CBM/Fundacao para a Ciencia e a Tecnologia/ GR - LA/P/0006/2020/Fundacao para a Ciencia e a Tecnologia/ GR - UID 00481/2025/Fundacao para a Ciencia e a Tecnologia/ GR - UID/50011/2025/Fundacao para a Ciencia e a Tecnologia/ PT - Journal Article DEP - 20261028 PL - United States TA - ACS Biomater Sci Eng JT - ACS biomaterials science & engineering JID - 101654670 SB - IM OTO - NOTNLM OT - 3D-printed microneedles OT - anti-inflammatory OT - antimicrobial OT - drug delivery systems OT - oral mucosal diseases OT - tissue healing OT - vat photopolymerization EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 15:03 PHST- 2026/08/05 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 15:03 [entrez] AID - 5460423 [pii] AID - 10.1021/acsbiomaterials.6c01471 [doi] PST - aheadofprint SO - ACS Biomater Sci Eng. 2026 Oct 28. doi: 10.1021/acsbiomaterials.6c01471. PMID- 42950139 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1536-2442 (Electronic) IS - 1536-2442 (Linking) VI - 26 IP - 5 DP - 2026 Sep 1 TI - Differential attraction of Lygus hesperus (Hemiptera: Miridae) to host plants in the laboratory. LID - 10.1093/jisesa/ieag117 [doi] LID - ieag117 AB - Lygus hesperus Knight is a generalist pest of alfalfa, strawberry, cotton, and other crops in western North America. Quantifying the relative olfactory preference of L. hesperus to different hosts and understanding the potential chemical basis of host selection could facilitate the monitoring and management of this serious pest. In this study, we examined the response of female L. hesperus to olfactory cues from 17 host plant species using flight tunnel and Y-tube olfactometer assays. We then selected 4 host plant species that elicited different responses along a gradient of low to high attraction and quantified their volatile profiles using dynamic headspace extractions and gas chromatography-mass spectrometry (GC-MS). Female L. hesperus responded to host plant volatiles in both behavioral assays with the highest proportion of female L. hesperus moving upwind when exposed to Medicago sativa. When given a choice between M. sativa and each other species individually in Y-tube assays, female L. hesperus preferred M. sativa relative to Artemisia vulgaris, Fragaria ananassa, Hordeum jubatum, Plantago major, and Capsella bursa-pastoris. The overall volatile profile of M. sativa differed statistically from the profile of C. bursa-pastoris and F. ananassa, with higher emission of various specific compounds, (Z)-beta-ocimene and (E)-beta-ocimene, in M. sativa. The information gathered in this study suggests a potential use of M. sativa as a trap for L. hesperus and points to specific plant volatiles to consider in the development of chemical lures for L. hesperus. CI - Published by Oxford University Press on behalf of Entomological Society of America 2026. FAU - Hetherington, Matthew C AU - Hetherington MC AUID- ORCID: 0000-0002-3307-620X AD - Department of Entomology, University of Wisconsin - Madison, Madison, WI, USA. FAU - Guedot, Christelle AU - Guedot C AUID- ORCID: 0000-0002-6558-9886 AD - Department of Entomology, University of Wisconsin - Madison, Madison, WI, USA. FAU - Brunet, Johanne AU - Brunet J AUID- ORCID: 0000-0003-4555-7411 AD - United States Department of Agriculture, Agricultural Research Service, Vegetable Crops Research Unit, Madison, WI, USA. AD - Brunet Research, Madison, WI, USA. LA - eng GR - 2017-70005-27332/National Institute of Food and Agriculture-Alfalfa Forage Research/ GR - UW-Madison Department of Entomology Lilian and Alex Feir Fellowship/ PT - Journal Article PL - United States TA - J Insect Sci JT - Journal of insect science (Online) JID - 101096396 RN - 0 (Volatile Organic Compounds) SB - IM MH - Animals MH - Female MH - *Heteroptera/physiology MH - *Volatile Organic Compounds MH - Gas Chromatography-Mass Spectrometry PMC - PMC13715977 OTO - NOTNLM OT - behavioral assays OT - floral scent OT - generalist herbivores OT - host preference OT - volatile organic compounds COIS- The authors have no relevant financial or non-financial interests to disclose. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 15:02 PHST- 2026/06/24 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 15:02 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 9004053 [pii] AID - ieag117 [pii] AID - 10.1093/jisesa/ieag117 [doi] PST - ppublish SO - J Insect Sci. 2026 Sep 1;26(5):ieag117. doi: 10.1093/jisesa/ieag117. PMID- 42950138 OWN - NLM STAT- Publisher LR - 20261028 IS - 1944-8252 (Electronic) IS - 1944-8244 (Linking) DP - 2026 Oct 28 TI - Electronic Structure Modulation and Electrochemically Induced Reconstruction of Ni-Doped Fe Oxyhydroxides for Overall Water Splitting. LID - 10.1021/acsami.6c16334 [doi] AB - Structurally analogous metal oxyhydroxides such as NiFeOOH and CoFeOOH lie at the pinnacle of the oxygen evolution reaction (OER) volcano plot for alkaline water electrolysis. However, stabilization of a single FeOOH phase is inherently challenging because of the small thermodynamic energy differences among FeOOH polymorphs and the phase selection being governed by crystallization kinetics. Herein, we report a heterovalent Ni doping strategy to stabilize beta-FeOOH structural morphology and enhance the local electric field, thereby tuning its electronic conductivity to promote OER activity. Moreover, Ni doping alters the interfacial structure in an optimal OH- ionic environment by lowering the reaction energy barriers at Fe active sites with a significantly lower adsorption free energy of 3.56 eV for the *OOH intermediate. Under cathodic polarization, NFO24/CFC undergoes in situ reconstruction, transforming bulky alpha-Fe2O3 NCs into hydroxylated rich FeOOH-like thin nanosheets. This structural evolution generates abundant active sites and facilitates charge-transfer kinetics, thereby accounting for its superior hydrogen evolution reaction (HER) activity. Consequently, NFO6/CFC delivers a high anodic current density with an OER overpotential of 355 mV, while NFO24/CFC exhibits superior HER performance with an overpotential of 272 mV at a current density of 10 mA cm-2 in alkaline media. Furthermore, the two-electrode assembly using NFO6/CFC parallelNFO24/CFC operates at a cell voltage of 1.87 V to drive overall water splitting and demonstrates long-term stability of 24 h at 10 mA cm-2 in alkaline conditions. CI - (c) 2026 American Chemical Society. FAU - Rhakho, Novuhulu AU - Rhakho N AD - Centre for Nano and Material Sciences, Jain (Deemed-to-be University), Jain Global Campus, Ramanagara, Bangalore, Karnataka562112, India. FAU - Yhobu, Zhoveta AU - Yhobu Z AUID- ORCID: 0000-0003-3401-6783 AD - Centre for Nano and Material Sciences, Jain (Deemed-to-be University), Jain Global Campus, Ramanagara, Bangalore, Karnataka562112, India. FAU - Muniswamy, Jeevitha AU - Muniswamy J AUID- ORCID: 0009-0000-5991-7946 AD - Centre for Nano and Material Sciences, Jain (Deemed-to-be University), Jain Global Campus, Ramanagara, Bangalore, Karnataka562112, India. FAU - Das, Arpita AU - Das A AD - Department of Physics, School of Applied Sciences, Kalinga Institute of Industrial Technology, Bhubaneswar, Odisha751024, India. FAU - Budagumpi, Srinivasa AU - Budagumpi S AUID- ORCID: 0000-0001-6591-7811 AD - Centre for Nano and Material Sciences, Jain (Deemed-to-be University), Jain Global Campus, Ramanagara, Bangalore, Karnataka562112, India. FAU - Austeria P, Muthu AU - Austeria P M AUID- ORCID: 0000-0002-8373-9032 AD - Centre for Nano and Material Sciences, Jain (Deemed-to-be University), Jain Global Campus, Ramanagara, Bangalore, Karnataka562112, India. FAU - Saxena, Manav AU - Saxena M AUID- ORCID: 0000-0003-4636-3942 AD - Centre for Nano and Material Sciences, Jain (Deemed-to-be University), Jain Global Campus, Ramanagara, Bangalore, Karnataka562112, India. FAU - Jadhav, Arvind H AU - Jadhav AH AUID- ORCID: 0000-0002-9128-1981 AD - Centre for Nano and Material Sciences, Jain (Deemed-to-be University), Jain Global Campus, Ramanagara, Bangalore, Karnataka562112, India. FAU - Samal, Akshaya K AU - Samal AK AUID- ORCID: 0000-0002-7623-3711 AD - Centre for Nano and Material Sciences, Jain (Deemed-to-be University), Jain Global Campus, Ramanagara, Bangalore, Karnataka562112, India. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - ACS Appl Mater Interfaces JT - ACS applied materials & interfaces JID - 101504991 SB - IM OTO - NOTNLM OT - HER OT - OER OT - binder free OT - carbon fiber cloth OT - doping OT - oxyhydroxide OT - reconstruction OT - water splitting EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 15:02 PHST- 2026/08/13 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 15:02 [entrez] AID - 5460427 [pii] AID - 10.1021/acsami.6c16334 [doi] PST - aheadofprint SO - ACS Appl Mater Interfaces. 2026 Oct 28. doi: 10.1021/acsami.6c16334. PMID- 42950124 OWN - NLM STAT- Publisher LR - 20261028 IS - 1724-6059 (Electronic) IS - 1121-8428 (Linking) DP - 2026 Oct 28 TI - Clinical and Laboratory Factors Associated with Renal Complications Following High-Voltage Electrical Burns: A Retrospective Cohort Study. LID - aajag269 [pii] LID - 10.1093/joneph/aajag269 [doi] AB - BACKGROUND: High-voltage electrical burns cause deep tissue injury, rhabdomyolysis, and acute kidney injury (AKI), but factors associated with renal complications are incompletely characterized. We investigated clinical and laboratory characteristics associated with dipstick-defined presumptive myoglobinuria, AKI, and renal replacement therapy (RRT) after high-voltage electrical burns. METHODS: This retrospective cohort included 37 male patients with high-voltage electrical burns (>/=1000 V) treated at a tertiary burn center. Demographics, injury severity, outcomes, surgical interventions, and admission and 48-hour laboratory parameters were compared using nonparametric univariable tests. Receiver operating characteristic (ROC) analyses explored the discriminatory performance of selected variables. As 48-hour serum creatinine contributed to AKI ascertainment, it was not considered an independent AKI biomarker. RESULTS: Dipstick-defined presumptive myoglobinuria was present in 30 patients (81.1%), AKI occurred in 9 (24.3%), and RRT was required in 3 (8.1%). Patients with AKI had greater TBSA and higher CK, serum myoglobin, and troponin I concentrations than patients without AKI. Among variables available at admission, CK showed the highest discrimination for AKI (AUC 0.925, 95% CI 0.822-0.993), followed by troponin I (AUC 0.919, 95% CI 0.814-0.992) and TBSA (AUC 0.907, 95% CI 0.776-0.992). The increase in serum creatinine over 48 hours was considered part of AKI ascertainment rather than an independent diagnostic marker. CONCLUSIONS: Renal complications after high-voltage electrical burns were associated with greater burn severity and more pronounced laboratory abnormalities. Among admission variables, CK, troponin I, and TBSA showed high discriminatory ability for AKI. These findings are exploratory and require confirmation in larger independent cohorts. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Italian Society of Nephrology. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Yigit, Ebral AU - Yigit E AUID- ORCID: 0000-0001-8766-0754 AD - Gazi Yasargil Training and Research Hospital, Diyarbakir, Turkey. FAU - Karayil, Ali Riza AU - Karayil AR AD - Van Training and Research Hospital, Department of General Surgery, Van, Turkey. FAU - Akelma, Hakan AU - Akelma H AD - Mardin Artuklu University, Mardin, Turkey. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - J Nephrol JT - Journal of nephrology JID - 9012268 SB - IM OTO - NOTNLM OT - Acute kidney injury OT - High-voltage electrical injury OT - Myoglobinuria OT - Rhabdomyolysis EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 14:43 PHST- 2026/07/06 00:00 [received] PHST- 2026/08/16 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:43 [entrez] AID - 9004074 [pii] AID - 10.1093/joneph/aajag269 [doi] PST - aheadofprint SO - J Nephrol. 2026 Oct 28:aajag269. doi: 10.1093/joneph/aajag269. PMID- 42950122 OWN - NLM STAT- Publisher LR - 20261028 IS - 1532-2548 (Electronic) IS - 0032-0889 (Linking) DP - 2026 Oct 28 TI - The Plant Ubiquitin System: From Identification of E2 and E3 enzymes to Molecular Mechanisms and Biological Function. LID - kiag799 [pii] LID - 10.1093/plphys/kiag799 [doi] AB - Ubiquitination is an essential post-translation modification involved in almost every cellular process and is critical for proper functioning of eukaryotic cells. The modification involves the covalent attachment of ubiquitin, a small, conserved peptide, to select proteins. One or more ubiquitin molecules may be attached in varying configurations, leading to a range of possible outcomes that regulate protein function, including changes in location, stability, and activity. Components of the ubiquitin system are highly expanded in plants relative to other eukaryotes and play major roles in almost all aspects of plant growth and development, from phytohormone perception and signalling, to maintaining nutrient homeostasis in the face of fluctuating environmental conditions. We begin this Centennial Review by highlighting two Plant Physiology papers that contributed to the identification and characterisation of ubiquitin enzymes. We then provide an update on our understanding of how plants use the ubiquitin system to link environmental change with plant growth and development. We first focus on recent advances in understanding how the structure and function of ubiquitin ligases are modulated by phytohormones and nutrient status. We then highlight emerging knowledge linking components of the ubiquitin system with biomolecular condensates, including stress granules, and discuss the potential functional implications. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of American Society of Plant Biologists. FAU - Bradley, James M AU - Bradley JM AUID- ORCID: 0000-0001-9579-6803 AD - Cell and Systems Biology, University of Toronto, Toronto ON Canada. FAU - Stone, Sophia L AU - Stone SL AUID- ORCID: 0000-0003-2140-6037 AD - Department of Biology, Dalhousie University, Halifax NS Canada. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Plant Physiol JT - Plant physiology JID - 0401224 SB - IM OTO - NOTNLM OT - Biomolecular condensates OT - Hormone Signalling OT - Nutrient Stress OT - Protein Degradation OT - Stress Granules OT - Ubiquitin Conjugating Enzymes OT - Ubiquitin Ligases OT - Ubiquitination EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 14:43 PHST- 2026/06/29 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:43 [entrez] AID - 9004080 [pii] AID - 10.1093/plphys/kiag799 [doi] PST - aheadofprint SO - Plant Physiol. 2026 Oct 28:kiag799. doi: 10.1093/plphys/kiag799. PMID- 42950110 OWN - NLM STAT- Publisher LR - 20261028 IS - 1536-3694 (Electronic) IS - 0163-4356 (Linking) DP - 2026 Oct 28 TI - Quantification of Antibiotic Concentration at the Site of Infection: Analytical Challenges and Current Practices. LID - 10.1097/FTD.0000000000001501 [doi] AB - BACKGROUND: Quantifying antibiotic concentrations at infection sites is challenging because of invasive sampling and complex matrices. Despite limited knowledge, target-site data are crucial for optimizing dosing, minimizing resistance, ensuring safer strategies, and reducing toxicity risks through adequate target attainment. METHODS: This review aims to provide an overview of the most commonly reported challenges associated with quantifying target-site concentrations of antibiotics. Antibiotics were selected as the focus because of their use in hospital and outpatient settings. An extensive literature search was conducted using Embase.com. After title and abstract screening of 828 articles, 398 articles were included for full-text screening, and 72 were included based on relevance to analytical challenges in target-site quantification. Reported challenges were categorized in subgroups: sampling difficulties, sample heterogeneity, unavailability of free drug concentrations, drug complexities, and others. RESULTS: Direct measurement of antibiotic concentrations at infection sites is constrained by invasive procedures and complex analytical methods. Key challenges include sampling heterogeneity, difficulty in obtaining samples, and the lack of free drug concentration data. Several opportunities have been proposed to address these challenges. CONCLUSIONS: We recommend updating international validation guidelines to include standardized tissue sampling protocols, encouraging the commercial sectors to develop reference standards and blank materials for alternative matrices, and promoting the use of minimally invasive techniques, such as microneedle biosensors, to improve the accuracy and safety of target-site quantification. These advances offer promising avenues to enhance antimicrobial therapy optimization despite current. CI - Copyright (c) 2026 The Author(s). Published by Wolters Kluwer Health, LLC. FAU - Bahmany, Soma AU - Bahmany S AD - Department of Hospital Pharmacy, Erasmus MC, University Medical Center, Rotterdam, the Netherlands. FAU - Holst, Amanda J AU - Holst AJ AD - Department of Hospital Pharmacy, Erasmus MC, University Medical Center, Rotterdam, the Netherlands. FAU - Bramer, Wichor M AU - Bramer WM AD - Medical Library, Erasmus MC-Erasmus University Medical Center, Rotterdam, the Netherlands. FAU - Ramautar, Rawi AU - Ramautar R AD - Leiden Academic Center for Drug Research, Leiden University, Leiden, the Netherlands; and. FAU - Flint, Robert B AU - Flint RB AD - Department of Hospital Pharmacy, Erasmus MC, University Medical Center, Rotterdam, the Netherlands. AD - Department of Pediatrics, Division of Neonatal and Pediatric Intensive Care, Erasmus MC Sophia Children's Hospital, Rotterdam, the Netherlands. FAU - Koch, Birgit C P AU - Koch BCP AD - Department of Hospital Pharmacy, Erasmus MC, University Medical Center, Rotterdam, the Netherlands. AD - CATOR, Center for Antimicrobial Treatment Optimization, Rotterdam, the Netherlands. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Ther Drug Monit JT - Therapeutic drug monitoring JID - 7909660 SB - IM OTO - NOTNLM OT - anti-infective agents OT - challenges OT - microdialysis OT - target-site quantification OT - tissue biopsy COIS- The authors declare no conflict of interest. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 14:23 PHST- 2025/12/19 00:00 [received] PHST- 2026/05/15 00:00 [accepted] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:23 [entrez] AID - 00007691-990000000-00518 [pii] AID - 10.1097/FTD.0000000000001501 [doi] PST - aheadofprint SO - Ther Drug Monit. 2026 Oct 28. doi: 10.1097/FTD.0000000000001501. PMID- 42950109 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261028 IS - 2817-1705 (Electronic) IS - 2817-1705 (Linking) VI - 5 DP - 2026 Oct 28 TI - Harnessing AI for Hypothyroidism Monitoring Using Wearable-Derived Heart Rate Data: Retrospective Cohort Study. PG - e86030 LID - 10.2196/86030 [doi] LID - e86030 AB - BACKGROUND: Hypothyroidism is a prevalent endocrine disorder characterized by an insidious onset and nonspecific symptoms, often leading to significant diagnostic delays. While biochemical thyroid function tests (TFTs) are the gold standard, their invasive nature and cost make them impractical for continuous, proactive screening of at-risk populations. Thyroid hormones exert direct chronotropic effects on the cardiovascular system, and their levels are closely correlated with heart rate (HR). Wearable devices capable of continuous HR monitoring offer a novel opportunity for noninvasive, digital phenotype-based detection. OBJECTIVE: This study aimed to develop and validate an AI-assisted system that uses wearable-derived sleep HR data as a digital monitoring tool for individuals at risk of hypothyroidism, helping identify those who warrant further confirmatory thyroid testing. METHODS: This retrospective cohort study used 1677 paired observations consisting of sleep HR data and serum TFT results, including free thyroxine (T4) and thyroid-stimulating hormone levels, from 413 participants (mean age 43.1, SD 9.7 y; women: n=314, 76%). Participants wore consumer-grade wearables (Fitbit) that recorded HR and sleep data. Each TFT result was paired with sleep HR data extracted from the 15 consecutive nights preceding the blood test. An AI system was developed using a gradient boosting algorithm (LightGBM) to classify thyroid status by integrating baseline TFT results with statistical and temporal HR features. The model's performance was evaluated using patient-level leave-one-out cross-validation. To examine the benefit of longitudinal data, performance was compared using 1, 2, or 3 prior paired observations as reference points. RESULTS: To ensure clinical relevance and avoid performance inflation from hyperthyroid cases, we prioritized the analysis of a subset containing only euthyroid and hypothyroid data (n=744 paired observations). In this clinically challenging subset, the system achieved an accuracy of 74.6%, with a sensitivity of 82% and a specificity of 73.5% using a single-reference data pair. Incorporating longitudinal HR patterns further enhanced performance; using 3 reference pairs improved accuracy to 78.1% (sensitivity 81.8% and specificity 77.7%). In the overall dataset (n=1677 paired observations), the system demonstrated an accuracy of 80.8%, with a sensitivity of 82% and a specificity of 80.8% using a single-reference pair. Notably, the system achieved high clinical safety by detecting 96.3% of severe overt hypothyroidism cases (free T4 <0.5 ng/dL). CONCLUSIONS: This AI-assisted system demonstrates the feasibility of a sensitive, noninvasive longitudinal monitoring tool for hypothyroidism, robustly identifying a high proportion of hypothyroid cases-particularly severe ones-to prioritize at-risk individuals for confirmatory blood tests. Given the predominantly female iatrogenic-withdrawal cohort, prospective sex-balanced validation in primary hypothyroidism is warranted. CI - (c) Kyubo Shin, Yewon Jung, Won Sang Yoo, Min Joo Kim, Tae Jung Oh, Joon Ho Moon, Sung Hye Kong, Won Woo Lee, Jae Hoon Moon. Originally published in JMIR AI (https://ai.jmir.org). FAU - Shin, Kyubo AU - Shin K AUID- ORCID: 0000-0002-0329-3166 AD - Thyroscope Inc, Ulsan, Republic of Korea. FAU - Jung, Yewon AU - Jung Y AUID- ORCID: 0009-0008-6210-0425 AD - Thyroscope Inc, Ulsan, Republic of Korea. FAU - Yoo, Won Sang AU - Yoo WS AUID- ORCID: 0000-0002-2314-4184 AD - Thyroscope Inc, Ulsan, Republic of Korea. AD - Department of Internal Medicine, Dankook University College of Medicine, Cheonan, Republic of Korea. FAU - Kim, Min Joo AU - Kim MJ AUID- ORCID: 0000-0002-9765-9340 AD - Department of Internal Medicine, Seoul National University Bundang Hospital and Seoul National University College of Medicine, 82, Gumi-ro 173 Beon-gil, Bundang-gu, Seongnam, 13620, Republic of Korea, 82 317877068. FAU - Oh, Tae Jung AU - Oh TJ AUID- ORCID: 0000-0002-5078-6123 AD - Department of Internal Medicine, Seoul National University Bundang Hospital and Seoul National University College of Medicine, 82, Gumi-ro 173 Beon-gil, Bundang-gu, Seongnam, 13620, Republic of Korea, 82 317877068. FAU - Moon, Joon Ho AU - Moon JH AUID- ORCID: 0000-0003-1897-0905 AD - Department of Internal Medicine, Seoul National University Bundang Hospital and Seoul National University College of Medicine, 82, Gumi-ro 173 Beon-gil, Bundang-gu, Seongnam, 13620, Republic of Korea, 82 317877068. FAU - Kong, Sung Hye AU - Kong SH AUID- ORCID: 0000-0002-8791-0909 AD - Department of Internal Medicine, Seoul National University Bundang Hospital and Seoul National University College of Medicine, 82, Gumi-ro 173 Beon-gil, Bundang-gu, Seongnam, 13620, Republic of Korea, 82 317877068. FAU - Lee, Won Woo AU - Lee WW AUID- ORCID: 0000-0002-9188-6921 AD - Department of Nuclear Medicine, Seoul National University Bundang Hospital and Seoul National University College of Medicine, Seongnam, Republic of Korea. FAU - Moon, Jae Hoon AU - Moon JH AUID- ORCID: 0000-0001-6327-0575 AD - Thyroscope Inc, Ulsan, Republic of Korea. AD - Department of Internal Medicine, Seoul National University Bundang Hospital and Seoul National University College of Medicine, 82, Gumi-ro 173 Beon-gil, Bundang-gu, Seongnam, 13620, Republic of Korea, 82 317877068. AD - Center for Artificial Intelligence in Healthcare, Seoul National University Bundang Hospital, Seongnam, Republic of Korea. LA - eng PT - Journal Article DEP - 20261028 PL - Canada TA - JMIR AI JT - JMIR AI JID - 9918645789006676 PMC - PMC13716157 OTO - NOTNLM OT - artificial intelligence OT - heart rate OT - hypothyroidism OT - machine learning OT - wearable device COIS- Conflicts of Interest: KS and YJ are employees of Thyroscope Inc. Jae Hoon Moon is a cofounder, executive, and shareholder of Thyroscope Inc, a company that owns patents related to this study. WSY serves as a medical advisor for Thyroscope Inc. MJK, TJO, Joon Ho Moon, SHK, and WWL have nothing to disclose. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 14:23 PHST- 2025/10/17 00:00 [received] PHST- 2026/09/07 00:00 [revised] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:23 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - v5i1e86030 [pii] AID - 86030 [pii] AID - 10.2196/86030 [doi] PST - epublish SO - JMIR AI. 2026 Oct 28;5:e86030. doi: 10.2196/86030. PMID- 42950105 OWN - NLM STAT- Publisher LR - 20261028 IS - 2326-6074 (Electronic) IS - 2326-6066 (Linking) DP - 2026 Oct 28 TI - Watch-and-Wait after Neoadjuvant PD-1 Blockade in Nonmetastatic dMMR/MSI-H Lower Rectal Cancer: A Phase 2 Trial (BASKET). LID - 10.1158/2326-6066.CIR-26-0730 [doi] AB - Neoadjuvant PD-1 blockade produces substantial tumor regression in mismatch repair-deficient or microsatellite instability-high (dMMR/MSI-H) locally advanced lower rectal cancer (LRC), but response assessment and the safety of watch-and-wait (W&W) remain uncertain. BASKET was an open-label, multicenter, single-arm, phase 2 study conducted at two Chinese centers (ClinicalTrials.gov, NCT04643041). Patients with non-metastatic dMMR/MSI-H LRC received six cycles of neoadjuvant sintilimab (200 mg every 3 weeks), followed by multimodal assessment including endoscopy, digital rectal examination, CEA, MRI, CT, and transrectal ultrasound-guided biopsy. Patients meeting protocol-defined clinical complete response (cCR) criteria entered W&W surveillance. Of 15 treated patients, 13 completed treatment and response assessment. All 13 per-protocol patients achieved cCR and entered W&W (13/13, 100%), corresponding to 13/15 (86.7%) in the intention-to-treat (ITT) population. Only 1 of 13 per-protocol patients (7.7%) achieved radiologic complete response on MRI; persistent radiographic abnormalities were adjudicated using the other response modalities. At a median follow-up of 37.7 months (range, 0.7-56.2) in the ITT population, one death occurred 0.7 months after treatment initiation, and no local recurrence or distant metastasis. The estimated 1-year disease-free and overall survival rates were both 93.3% (95% CI, 68.1%-99.8%). Among the 13 per-protocol patients no local recurrence, distant metastasis, or death was observed. No grade 3 or higher immune-related adverse events or biopsy-related complications occurred. These findings suggest that W&W may be feasible in carefully selected patients following rigorous multimodal response assessment and with close surveillance; however, the incremental value of biopsy and the oncologic equivalence of W&W to surgery remain unproven. FAU - Pei, Fengyun AU - Pei F AUID- ORCID: 0009-0004-1835-7769 AD - Sixth Affiliated Hospital of Sun Yat-sen University Guang zhou, Guang dong China. ROR: https://ror.org/005pe1772 FAU - Yao, Qi-Jun AU - Yao QJ AUID- ORCID: 0009-0000-9725-4506 AD - Sixth Affiliated Hospital of Sun Yat-sen University China. ROR: https://ror.org/005pe1772 FAU - Zhao, Yandong AU - Zhao Y AUID- ORCID: 0000-0001-8661-0229 AD - Sixth Affiliated Hospital of Sun Yat-sen University China. ROR: https://ror.org/005pe1772 FAU - He, Fang AU - He F AUID- ORCID: 0000-0002-8165-7914 AD - Sixth Affiliated Hospital of Sun Yat-sen University China. ROR: https://ror.org/005pe1772 FAU - Zhang, Linsen AU - Zhang L AUID- ORCID: 0009-0006-3875-2082 AD - The Third Affiliated Hospital of Kunming Medical University，Yunnan CancerHospital，Peking University Cancer Hospital Yunnan Kunming, Yunnan China. FAU - Liu, Zhimin AU - Liu Z AUID- ORCID: 0000-0003-1749-9322 AD - Sixth Affiliated Hospital of Sun Yat-sen University guangzhou China. ROR: https://ror.org/005pe1772 FAU - Zhong, Qinghua AU - Zhong Q AUID- ORCID: 0000-0001-6199-5599 AD - Sixth Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong China. ROR: https://ror.org/005pe1772 FAU - Xie, Pei-Yi AU - Xie PY AUID- ORCID: 0009-0006-0970-4823 AD - Sixth Affiliated Hospital of Sun Yat-sen University China. ROR: https://ror.org/005pe1772 FAU - Cai, Zerong AU - Cai Z AUID- ORCID: 0000-0002-4514-7116 AD - Sixth Affiliated Hospital of Sun Yat-sen University Guangzhou China. ROR: https://ror.org/005pe1772 FAU - Zhou, Jiaming AU - Zhou J AUID- ORCID: 0000-0001-8412-0146 AD - Sixth Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong China. ROR: https://ror.org/005pe1772 FAU - Tan, Shuyun AU - Tan S AUID- ORCID: 0000-0002-2099-3674 AD - Sixth Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong China. ROR: https://ror.org/005pe1772 FAU - Hu, Huanxin AU - Hu H AUID- ORCID: 0009-0009-9335-9971 AD - Sixth Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong China. ROR: https://ror.org/005pe1772 FAU - Lu, Xianglong AU - Lu X AUID- ORCID: 0009-0006-8243-9704 AD - The Third Affiliated Hospital of Kunming Medical University Kunming, Yunnan China. FAU - Wang, Menghan AU - Wang M AUID- ORCID: 0009-0005-9783-2229 AD - Sixth Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong China. ROR: https://ror.org/005pe1772 FAU - Shi, Lishuo AU - Shi L AUID- ORCID: 0000-0002-0715-9941 AD - Sixth Affiliated Hospital of Sun Yat-sen University Guangzhou, Guangdong China. ROR: https://ror.org/005pe1772 FAU - Ling, Li AU - Ling L AUID- ORCID: 0000-0003-3292-274X AD - Sun Yat-sen University Guangzhou China. ROR: https://ror.org/0064kty71 FAU - Liu, Ping AU - Liu P AUID- ORCID: 0009-0001-1364-015X AD - Yunnan Cancer Hospital Kunming, Yunnan China. FAU - Huang, Jun AU - Huang J AUID- ORCID: 0000-0002-1891-8237 AD - Sixth Affiliated Hospital of Sun Yat-sen University Guang zhou, Guang dong China. ROR: https://ror.org/005pe1772 LA - eng SI - ClinicalTrials.gov/NCT04643041 PT - Journal Article DEP - 20261028 PL - United States TA - Cancer Immunol Res JT - Cancer immunology research JID - 101614637 SB - IM EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 14:23 PHST- 2026/10/28 00:00 [accepted] PHST- 2026/06/21 00:00 [received] PHST- 2026/08/02 00:00 [revised] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:23 [entrez] AID - 789701 [pii] AID - 10.1158/2326-6066.CIR-26-0730 [doi] PST - aheadofprint SO - Cancer Immunol Res. 2026 Oct 28. doi: 10.1158/2326-6066.CIR-26-0730. PMID- 42950104 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1553-7358 (Electronic) IS - 1553-734X (Print) IS - 1553-734X (Linking) VI - 22 IP - 10 DP - 2026 Oct TI - Modeling reaction-diffusion processes in simplified to complex astroglial branchlet geometries using different simulation tools. PG - e1014878 LID - 10.1371/journal.pcbi.1014878 [doi] LID - e1014878 AB - Computational modeling of spatial biochemical processes in neuroscience is increasingly important as advances in experimental and imaging techniques provide detailed insights into cellular organization. Reaction-diffusion models are commonly used to study the diffusion and interactions of ions and molecules within neuronal cells, and multiple open-access simulation platforms have been developed to support such analysis. However, a comprehensive analysis of commonly used simulation tools, including MCell, STEPS, NeuroRD, and NEURON, remains lacking, particularly for realistic glial cell geometries, as these tools were originally developed for neurons. Here, we present a systematic analysis of these four simulation environments by implementing equivalent calcium diffusion and calcium-calmodulin reaction-diffusion models across a range of spatial domains. These include simplified geometries (cylinders and rectangular cuboids) as well as biologically realistic astroglial morphologies derived from electron microscopy data, including mouse Bergmann glia and mouse and human cortical astrocytes. Using matched kinetic parameters and diffusion constants, we examine simulation outputs and morphological variations across platforms under comparable conditions. We find that all tools produce overall consistent results for the systems considered, indicating that each can reliably capture diffusion and reaction-diffusion dynamics in simplified and complex geometries. At the same time, we show that the structural heterogeneity of branchlets from human astrocytes and mouse Bergmann glia gives rise to distinct calcium signaling, demonstrating that fine-scale morphology critically shapes astroglial dynamics and must be accurately captured by simulation frameworks. In addition, notable differences emerge in geometry representation, implementation strategies, computational aspects, and usability of the tools. By highlighting key trade-offs between geometric fidelity, flexibility, and computational cost in modeling intracellular biochemical processes, these findings provide practical guidance for selecting appropriate simulation tools. More broadly, our results support the use of reaction-diffusion modeling in morphologically realistic astrocytic environments and contribute to enabling mechanistic studies of localized signaling events at synaptic and perisynaptic sites. CI - Copyright: (c) 2026 Keto et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Keto, Laura AU - Keto L AUID- ORCID: 0000-0003-4649-1805 AD - Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland. FAU - Seppala, Saana AU - Seppala S AUID- ORCID: 0009-0002-6709-2256 AD - Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland. FAU - Ndubuaku, Derek AU - Ndubuaku D AUID- ORCID: 0009-0000-7838-1075 AD - Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland. FAU - Maki, Annika AU - Maki A AUID- ORCID: 0009-0003-9314-183X AD - Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland. FAU - Maki-Marttunen, Tuomo AU - Maki-Marttunen T AD - Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland. FAU - Linne, Marja-Leena AU - Linne ML AD - Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland. FAU - Manninen, Tiina AU - Manninen T AUID- ORCID: 0000-0002-0456-1185 AD - Faculty of Medicine and Health Technology, Tampere University, Tampere, Finland. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - PLoS Comput Biol JT - PLoS computational biology JID - 101238922 RN - SY7Q814VUP (Calcium) RN - 0 (Calmodulin) SB - IM MH - *Astrocytes/cytology/metabolism/physiology MH - Animals MH - Humans MH - Computer Simulation MH - Mice MH - Diffusion MH - Calcium/metabolism MH - Computational Biology MH - *Models, Neurological MH - *Models, Biological MH - Calmodulin/metabolism PMC - PMC13716318 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 14:22 PHST- 2026/04/13 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:22 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - PCOMPBIOL-D-26-00873 [pii] AID - 10.1371/journal.pcbi.1014878 [doi] PST - epublish SO - PLoS Comput Biol. 2026 Oct 28;22(10):e1014878. doi: 10.1371/journal.pcbi.1014878. eCollection 2026 Oct. PMID- 42950082 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1097-4652 (Electronic) IS - 0021-9541 (Linking) VI - 241 IP - 10 DP - 2026 Oct TI - Carbonic Anhydrases in Sarcoma: Resetting the Tumor's Survival Script. LID - e70249 [pii] LID - 10.1002/jcp.70249 [doi] AB - Carbonic anhydrases (CA) are zinc-containing metalloenzymes that catalyze the reversible hydration of carbon dioxide, playing a central role in pH homeostasis. In aggressive sarcomas, including osteosarcoma and Ewing sarcoma, specific CA isoforms are exploited to establish a pH gradient characterized by an alkaline cytoplasm and acidic extracellular microenvironment. This gradient promotes tumor cell survival under hypoxia, facilitates invasion and metastasis, and drives resistance to conventional therapies. High levels of CA II and CA IX expression are associated with worse 5-year survival in patients diagnosed with Ewing Sarcoma and osteosarcoma, respectively. Carbonic anhydrase inhibitors have evolved from non-selective sulfonamides to isoform-selective agents, such as the clinically advanced SLC-0111, which preferentially targets CA IX. Beyond direct cytotoxicity, carbonic anhydrase inhibitors exert multifaceted antitumor effects: they reverse pathological pH gradients, overcome pH-dependent chemoresistance, suppress invasive potential, and induce distinct cell death pathways including apoptosis and ferroptosis. Thus, CA inhibitors hold considerable promise for use in combination therapy, especially chemotherapy and immunotherapy. Clinical trials of CA inhibitors in advanced solid tumors have established favorable safety profiles, supporting their evaluation in CA-enriched sarcoma populations. This review summarizes the mechanistic rationale for targeting carbonic anhydrases in osteosarcoma and Ewing sarcoma, highlighting the therapeutic potential of CA inhibitors as part of combination regimens to overcome treatment resistance and improve patient outcomes. CI - (c) 2026 Wiley Periodicals LLC. FAU - Fayzullina, Daria R AU - Fayzullina DR AUID- ORCID: 0000-0002-0800-3869 AD - Laboratory for Molecular and Cellular Tumor Treatment, Institute for Regenerative Medicine, I.M. Sechenov First Moscow State Medical University, Moscow, Russia. FAU - Loginova, Nina P AU - Loginova NP AUID- ORCID: 0009-0002-7631-8149 AD - Laboratory for Molecular and Cellular Tumor Treatment, Institute for Regenerative Medicine, I.M. Sechenov First Moscow State Medical University, Moscow, Russia. FAU - Navruzbekova, Alina R AU - Navruzbekova AR AUID- ORCID: 0009-0000-7679-8926 AD - Laboratory for Molecular and Cellular Tumor Treatment, Institute for Regenerative Medicine, I.M. Sechenov First Moscow State Medical University, Moscow, Russia. FAU - Timashev, Peter S AU - Timashev PS AD - Laboratory for Molecular and Cellular Tumor Treatment, Institute for Regenerative Medicine, I.M. Sechenov First Moscow State Medical University, Moscow, Russia. AD - Department of Biological Chemistry, Sechenov University, Moscow, Russia. FAU - Ulasov, Ilya V AU - Ulasov IV AUID- ORCID: 0000-0002-0818-0363 AD - Laboratory for Molecular and Cellular Tumor Treatment, Institute for Regenerative Medicine, I.M. Sechenov First Moscow State Medical University, Moscow, Russia. LA - eng PT - Journal Article PT - Review PL - United States TA - J Cell Physiol JT - Journal of cellular physiology JID - 0050222 RN - 0 (Carbonic Anhydrase Inhibitors) RN - EC 4.2.1.1 (Carbonic Anhydrases) SB - IM MH - Humans MH - *Carbonic Anhydrase Inhibitors/therapeutic use MH - *Carbonic Anhydrases/metabolism/genetics MH - Animals MH - *Osteosarcoma/drug therapy/pathology/enzymology MH - *Sarcoma/drug therapy/pathology/enzymology MH - Hydrogen-Ion Concentration MH - *Sarcoma, Ewing/drug therapy/pathology/enzymology MH - Tumor Microenvironment/drug effects MH - Cell Survival/drug effects OTO - NOTNLM OT - cancer OT - carbonic anhydrases OT - pH cellular balance OT - sarcoma EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 CRDT- 2026/10/28 14:13 PHST- 2026/09/15 00:00 [revised] PHST- 2026/05/22 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:13 [entrez] AID - e70249 [pii] AID - 10.1002/jcp.70249 [doi] PST - ppublish SO - J Cell Physiol. 2026 Oct;241(10):e70249. doi: 10.1002/jcp.70249. PMID- 42950072 OWN - NLM STAT- Publisher LR - 20261028 IS - 1536-0911 (Electronic) IS - 1536-0903 (Linking) DP - 2026 Oct 27 TI - Predictors and Risk Factors of Necrotizing Enterocolitis: An Integrative Review. LID - 10.1097/ANC.0000000000001441 [doi] AB - BACKGROUND: Necrotizing enterocolitis (NEC) is associated with high morbidity and mortality among preterm infants. Early intervention is critical, but existing predictors and risk factors remain inconsistent. Integrating bedside nursing assessments with machine learning (ML) may enable prediction of NEC before deterioration. PURPOSE: This integrative review synthesizes evidence on predictors and risk factors of NEC in very low birth weight (VLBW; <1500 g) infants to guide ML-based prediction models, support nursing assessment, and support early clinical decision making. DATA SOURCES: MEDLINE, CINAHL, and Embase. STUDY SELECTION: We included quantitative studies published from 2015 to 2025, involving VLBW infants <32 weeks gestation and examining NEC predictors or risk factors. From 405 records, 325 abstracts were screened, and 14 studies met inclusion criteria. DATA EXTRACTION: Two reviewers independently assessed full texts, resolving differences through consensus. Data were organized thematically using an inductive approach with a predetermined extraction table. Study quality was evaluated with a Joanna Briggs Institute tool. RESULTS: Across 14 studies, 55 predictors or risk factors were identified. Gestational age, birth weight, and human milk exposure were consistently associated with NEC risk. Other maternal, antenatal, delivery, nutritional, social, and clinical variables varied widely, with only 16 factors appearing in multiple studies. Most studies used retrospective cohort designs, and one incorporated ML methods. IMPLICATIONS FOR PRACTICE AND RESEARCH: Nurses are central to NEC prevention through human milk promotion, risk surveillance, and bedside advocacy. Future NEC prediction should integrate dynamic, contextual variables with standardized definitions and validation across diverse settings. CI - Copyright (c) 2026 by The National Association of Neonatal Nurses. FAU - Fordham, Keliy AU - Fordham K AUID- ORCID: 0000-0002-0286-596 AD - Author Affiliations: College of Nursing, University of Florida, Gainesville, FL (Fordham, Monk, Bjarnadottir, Yao, and Parker); Tampa General Hospital, Tampa, Florida (Cousin); and College of Industrial and Systems Engineering, University of Florida, Gainesville, Florida (Zhong). FAU - Monk, Angela AU - Monk A FAU - Bjarnadottir, Ragnhildur I AU - Bjarnadottir RI FAU - Cousin, Lakeshia AU - Cousin L FAU - Zhong, Xiang AU - Zhong X FAU - Yao, Yingwei AU - Yao Y FAU - Parker, Leslie A AU - Parker LA LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Adv Neonatal Care JT - Advances in neonatal care : official journal of the National Association of Neonatal Nurses JID - 101125644 SB - IM OTO - NOTNLM OT - infant OT - machine learning OT - necrotizing enterocolitis OT - nursing assessment OT - risk factors OT - very low birth weight EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 14:04 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:04 [entrez] AID - 00149525-990000000-00327 [pii] AID - 10.1097/ANC.0000000000001441 [doi] PST - aheadofprint SO - Adv Neonatal Care. 2026 Oct 27. doi: 10.1097/ANC.0000000000001441. PMID- 42950067 OWN - NLM STAT- Publisher LR - 20261028 IS - 2160-1836 (Electronic) IS - 2160-1836 (Linking) DP - 2026 Oct 28 TI - Genomic prediction enables selection for improved fruit ripening traits in northern highbush blueberry (Vaccinium corymbosum L.) breeding populations. LID - jkag271 [pii] LID - 10.1093/g3journal/jkag271 [doi] AB - Blueberry (Vaccinium corymbosum L.) breeding is a time-consuming, complex, and labor-intensive process due to the long-lived woody perennial nature of blueberries and their autotetraploid (2n = 4x = 48) genome. Blueberry breeders have recently adopted and implemented molecular tools for enhancing breeding efficiency and accuracy. Genomic prediction (GP) utilizing genome-wide molecular marker information to predict breeding values of untested genotypes could help accelerate development of adapted varieties. GP and its implementation through genomic selection is an attractive accompaniment to traditional breeding for its potential to improve selection efficiency, particularly for complex polygenic traits. In this study, we utilized a low-cost ($15/sample) mid-density genetic marker platform developed for blueberries by the Breeding Insight initiative to test the first application of genomic prediction in a breeding population of Northern Highbush Blueberries in the Pacific Northwest (PNW), United States. We predicted breeding values for 18 traits related to fruit quality, timing of flowering, and fruit ripening time using 1,822 markers and phenotype data from 4,320 seedlings. Within-population cross-validation indicated moderate to moderately high prediction accuracy (0.6 to 0.8). In contrast, genome-wide association study (GWAS) identified significant markers with small effect (0.50% < phenotype variance explained (PVE) < 2.5%) for 14 traits, while genomic prediction regularly exceeded 0.60 for most traits in most years. Low GWAS signal and strong genomic prediction performance for several traits are consistent with predominantly polygenic trait architecture in this breeding population, where prediction benefits more from genome-wide relatedness and cumulative small effects than from a small number of detectable major loci. This study demonstrates that related individuals can be predicted when phenotypes from the same environment or year structure help estimate marker effects. These results support the potential for genomic prediction to assist early-stage selection in the US Department of Agriculture-Agricultural Research Service PNW blueberry breeding program, with possible reductions in time and phenotyping burden pending future validation in independent cohorts and breeding cycles. CI - Published by Oxford University Press on behalf of The Genetics Society of America 2026. FAU - Hislop, Lillian M AU - Hislop LM AUID- ORCID: 0000-0002-0043-5325 AD - United States Department of Agriculture-Agricultural Research Service, Horticultural Crops Production and Genetic Improvement Research Unit, 3420 NW Orchard Ave., Corvallis, OR 97330, United States. AD - Research Department, Savanna Institute, 2453 Atwood Ave. Suite 209, Madison, WI 53704, United States. FAU - Anderson, Todd AU - Anderson T AUID- ORCID: 0009-0001-2966-2302 AD - Department of Horticulture, Oregon State University, 2750 SW Campus Way, Corvallis, OR 97331, United States. FAU - Rhoades, Kathleen AU - Rhoades K AUID- ORCID: 0009-0004-9172-2443 AD - Research Department, Savanna Institute, 2453 Atwood Ave. Suite 209, Madison, WI 53704, United States. FAU - Bassil, Nahla AU - Bassil N AUID- ORCID: 0000-0001-8625-2740 AD - United States Department of Agriculture-Agricultural Research Service, National Clonal Germplasm Repository, 33447 Peoria Rd., Corvallis, OR 97333, United States. FAU - Zhao, Dongyan AU - Zhao D AUID- ORCID: 0000-0002-2080-8416 AD - Breeding Insight, University of Florida, Institute of Food and Agricultural Sciences, PO Box 110695, Gainesville, FL 32611, United States. FAU - Beil, Craig T AU - Beil CT AUID- ORCID: 0000-0002-1756-9987 AD - Breeding Insight, University of Florida, Institute of Food and Agricultural Sciences, PO Box 110695, Gainesville, FL 32611, United States. FAU - Sheehan, Moira J AU - Sheehan MJ AUID- ORCID: 0000-0002-3400-2165 AD - Breeding Insight, University of Florida, Institute of Food and Agricultural Sciences, PO Box 110695, Gainesville, FL 32611, United States. FAU - Luby, Claire H AU - Luby CH AUID- ORCID: 0000-0002-4954-0468 AD - United States Department of Agriculture-Agricultural Research Service, Horticultural Crops Production and Genetic Improvement Research Unit, 3420 NW Orchard Ave., Corvallis, OR 97330, United States. AD - Department of Plant Sciences and Plant Pathology, Montana State University, Plant BioScience Building, Bozeman, MT 59715, United States. FAU - Hardigan, Michael A AU - Hardigan MA AUID- ORCID: 0000-0001-6926-7561 AD - United States Department of Agriculture-Agricultural Research Service, Horticultural Crops Production and Genetic Improvement Research Unit, 3420 NW Orchard Ave., Corvallis, OR 97330, United States. FAU - Loarca, Jenyne AU - Loarca J AUID- ORCID: 0000-0002-3661-3795 AD - United States Department of Agriculture-Agricultural Research Service, Horticultural Crops Production and Genetic Improvement Research Unit, 3420 NW Orchard Ave., Corvallis, OR 97330, United States. LA - eng GR - 2072-21000-060-000D/USDA-ARS CRIS/ GR - Genotyping costs/ GR - SCR_026645/Breeding Insight/ GR - 8062-21000-043-004-A/Cornell University under Cooperative Agreements/ GR - 8062-21000-052-002-A/Cornell University under Cooperative Agreements/ GR - 8062-21000-052-003-A/Cornell University under Cooperative Agreements/ GR - 8062-21000-052-020-A/University of Florida, Gainesville, under a Cooperative Agreement/ GR - Research data/ GR - Breeding Insight/ GR - USDA Agricultural Research Service/ GR - SCINet/ GR - 0201-88888-003-000D/ARS/ GR - 0201-88888-002-000D/ARS/ PT - Journal Article DEP - 20261028 PL - England TA - G3 (Bethesda) JT - G3 (Bethesda, Md.) JID - 101566598 SB - IM OTO - NOTNLM OT - Bayesian genotyping OT - clonal crop breeding OT - molecular breeding OT - phenology OT - polyploid breeding OT - vaccinium EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 14:04 PHST- 2026/05/18 00:00 [received] PHST- 2026/08/19 00:00 [accepted] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:04 [entrez] AID - 9004044 [pii] AID - 10.1093/g3journal/jkag271 [doi] PST - aheadofprint SO - G3 (Bethesda). 2026 Oct 28:jkag271. doi: 10.1093/g3journal/jkag271. PMID- 42950054 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2375-2548 (Electronic) IS - 2375-2548 (Linking) VI - 12 IP - 44 DP - 2026 Oct 30 TI - Wdr82-COMPASS coordinates Foxp3 and TCR signaling to sustain T(reg) cell control of microbiota-driven intestinal inflammation. PG - eaeh9188 LID - 10.1126/sciadv.aeh9188 [doi] LID - eaeh9188 AB - Regulatory T cells (T(reg) cells) maintain immune tolerance, but how broadly acting epigenetic pathways are selectively used by T(reg) cells versus conventional T cells (T(conv) cells) remains unclear. Here, we identify the COMPASS-trimethylation of histone H3 lysine 4 (H3K4me3) pathway, through its Wdr82 component, as a key epigenetic regulator that preferentially supports T(reg) cell development and function. Although Wdr82 deficiency broadly impairs T cell activation, T cell-specific deletion paradoxically causes progressive, microbiota-dependent intestinal inflammation and expansion of inflammatory T(conv) cell programs. This pathology is prevented by microbiome depletion or transfer of wild-type T(reg) cells, indicating a primary defect in T(reg) cell-mediated tolerance. Mechanistically, Wdr82/COMPASS promotes Foxp3 induction, maintains H3K4me3 and chromatin accessibility at T(reg) cell-associated genes, and restrains effector programs within the T(reg) cell lineage. Foxp3 coordinates with COMPASS components in a T cell receptor/costimulation-dependent manner, linking antigen receptor signaling to chromatin regulation. Together, these findings reveal selective integration of a broadly active epigenetic pathway into the Foxp3-centered regulatory hierarchy that sustains T(reg) cell control of microbiota-driven intestinal inflammation. FAU - Huang, Wenjun AU - Huang W AUID- ORCID: 0009-0005-0002-3426 AD - Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Li, Jun AU - Li J AUID- ORCID: 0000-0002-2611-8918 AD - Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - He, Minghong AU - He M AUID- ORCID: 0000-0001-9978-7218 AD - Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Xu, Beisi AU - Xu B AUID- ORCID: 0000-0003-0099-858X AD - Center for Applied Bioinformatics, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Rosikiewicz, Wojciech AU - Rosikiewicz W AUID- ORCID: 0000-0001-7031-3430 AD - Center for Applied Bioinformatics, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Hao, Xiaolei AU - Hao X AUID- ORCID: 0000-0003-3413-2338 AD - Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Jiang, Menglin AU - Jiang M AUID- ORCID: 0000-0002-1660-9916 AD - Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Bai, Lu AU - Bai L AUID- ORCID: 0000-0002-8779-336X AD - Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Zhang, Siwei AU - Zhang S AUID- ORCID: 0000-0002-1646-063X AD - Center for Applied Bioinformatics, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Li, Lei AU - Li L AUID- ORCID: 0000-0002-8828-5199 AD - Center for Applied Bioinformatics, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Qi, Wenjie AU - Qi W AD - Center for Applied Bioinformatics, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Choudhary, Vivek AU - Choudhary V AD - Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Jheng, Min-Jhen AU - Jheng MJ AD - Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Chang, Ti-Cheng AU - Chang TC AUID- ORCID: 0000-0001-5302-9147 AD - Center for Applied Bioinformatics, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Janke, Laura J AU - Janke LJ AUID- ORCID: 0000-0002-9871-6239 AD - Department of Pathology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Pruett-Miller, Shondra M AU - Pruett-Miller SM AUID- ORCID: 0000-0002-3793-585X AD - Center for Advanced Genome Engineering, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Kodali, Kiran AU - Kodali K AUID- ORCID: 0000-0002-0854-1405 AD - Center for Proteomics and Metabolomics, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Yuan, Zuo-Fei AU - Yuan ZF AUID- ORCID: 0000-0003-1252-6766 AD - Center for Proteomics and Metabolomics, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - High, Anthony A AU - High AA AUID- ORCID: 0000-0003-2323-5649 AD - Center for Proteomics and Metabolomics, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Jin, Hongjian AU - Jin H AUID- ORCID: 0000-0003-3833-7170 AD - Center for Applied Bioinformatics, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Li, Chunliang AU - Li C AUID- ORCID: 0000-0002-5938-5510 AD - Department of Tumor Cell Biology, 4, Memphis, TN 38105, USA. FAU - Feng, Yongqiang AU - Feng Y AUID- ORCID: 0000-0002-2320-5063 AD - Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Sci Adv JT - Science advances JID - 101653440 RN - 0 (Forkhead Transcription Factors) RN - 0 (Foxp3 protein, mouse) RN - 0 (Receptors, Antigen, T-Cell) RN - 0 (Histones) SB - IM MH - *T-Lymphocytes, Regulatory/immunology/metabolism MH - *Forkhead Transcription Factors/metabolism/genetics MH - Animals MH - *Signal Transduction MH - Mice MH - *Receptors, Antigen, T-Cell/metabolism MH - Histones/metabolism MH - Epigenesis, Genetic MH - Lymphocyte Activation MH - *Inflammation/metabolism/immunology MH - Intestines/immunology/microbiology MH - Mice, Knockout MH - *Gastrointestinal Microbiome/immunology PMC - PMC13715726 COIS- The authors declare that they have no competing interests. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 14:03 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:03 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - aeh9188 [pii] AID - 10.1126/sciadv.aeh9188 [doi] PST - ppublish SO - Sci Adv. 2026 Oct 30;12(44):eaeh9188. doi: 10.1126/sciadv.aeh9188. Epub 2026 Oct 28. PMID- 42950047 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2375-2548 (Electronic) IS - 2375-2548 (Linking) VI - 12 IP - 44 DP - 2026 Oct 30 TI - Cultural norms of exogamy and mobility shape genetic evolution of Central African hunter-gatherers. PG - eaeh1658 LID - 10.1126/sciadv.aeh1658 [doi] LID - eaeh1658 AB - Through a hunting and gathering lifestyle, humans have managed to thrive across all terrestrial ecosystems. A key adaptive feature enabling this ecological success is the ability of hunter-gatherer societies to maintain high levels of genetic diversity despite ecological and demographic shocks, but the mechanisms underlying this resilience are poorly understood. Here, we integrate genomic, demographic, mobility, and ethnographic data from two Central African hunter-gatherer populations to show that genetic diversity emerges from interacting effects of population size, mobility, and cultural norms governing marriage. We first demonstrate direct selection against background homozygosity: Even modest increases in runs of homozygosity, in the near absence of close-kin marriages, are associated with reduced reproductive success. Despite regional differences in effective population size, clustering of relatives, sedentism, and exogamy rules, overall levels of homozygosity are similarly low in both populations. These shared genetic outcomes are achieved through distinct strategies: In one region, strict exogamy combined with high lifetime mobility limits local relatedness, whereas in the other, more relaxed exogamy norms are offset by increased male mate-search distances that reduce offspring homozygosity. Together, our results show that human populations flexibly adjust mobility and social norms to demographic constraints to preserve genetic diversity and avoid fitness costs, revealing culture as a central component of human adaptation. FAU - Padilla-Iglesias, Cecilia AU - Padilla-Iglesias C AUID- ORCID: 0000-0003-1302-5955 AD - HUMAN ORIGINS-Cluster of Excellence for Integrative Human Origins Studies (EXC 3101), University of Tubingen, Tubingen, Germany. AD - Evolutionary Ecology Group, Department of Zoology, University of Cambridge, Cambridge, UK. AD - Emmanuel College, University of Cambridge, Cambridge, UK. AD - McDonald Institute for Archaeological Research, University of Cambridge, Cambridge, UK. FAU - Nganga, Dieudrice AU - Nganga D AUID- ORCID: 0009-0008-6311-9400 AD - Human Evolutionary Ecology Group, Department of Evolutionary Anthropology, University of Zurich, Zurich, Switzerland. AD - Marien Ngouabi University, Brazzaville, Republic of Congo. FAU - Amboulou, Eustache AU - Amboulou E AD - Human Evolutionary Ecology Group, Department of Evolutionary Anthropology, University of Zurich, Zurich, Switzerland. AD - Marien Ngouabi University, Brazzaville, Republic of Congo. FAU - Ruf, Juliette AU - Ruf J AD - Human Evolutionary Ecology Group, Department of Evolutionary Anthropology, University of Zurich, Zurich, Switzerland. FAU - Gerbault, Pascale AU - Gerbault P AUID- ORCID: 0000-0003-3824-0870 AD - Laboratory of Anthropology, Genetics and Peopling History, Department of Genetics and Evolution, University of Geneva, Geneva, Switzerland. FAU - Docquier, Mylene AU - Docquier M AD - iGE3 Genomics Platform, University of Geneva, Geneva, Switzerland. FAU - Averti, Suspense Ifo AU - Averti SI AUID- ORCID: 0000-0003-3351-9987 AD - Marien Ngouabi University, Brazzaville, Republic of Congo. FAU - Vinicius, Lucio AU - Vinicius L AUID- ORCID: 0000-0002-9396-3249 AD - Human Evolutionary Ecology Group, Department of Evolutionary Anthropology, University of Zurich, Zurich, Switzerland. FAU - Manica, Andrea AU - Manica A AUID- ORCID: 0000-0003-1895-450X AD - HUMAN ORIGINS-Cluster of Excellence for Integrative Human Origins Studies (EXC 3101), University of Tubingen, Tubingen, Germany. AD - Evolutionary Ecology Group, Department of Zoology, University of Cambridge, Cambridge, UK. FAU - Migliano, Andrea AU - Migliano A AUID- ORCID: 0000-0003-4364-2735 AD - Human Evolutionary Ecology Group, Department of Evolutionary Anthropology, University of Zurich, Zurich, Switzerland. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Sci Adv JT - Science advances JID - 101653440 SB - IM MH - Humans MH - Male MH - Genetic Variation MH - Female MH - Genetics, Population MH - *Evolution, Molecular MH - Central African People MH - Homozygote MH - *Culture MH - Marriage MH - Population Density PMC - PMC13715702 COIS- The authors declare that they have no competing interests. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 14:03 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:03 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - aeh1658 [pii] AID - 10.1126/sciadv.aeh1658 [doi] PST - ppublish SO - Sci Adv. 2026 Oct 30;12(44):eaeh1658. doi: 10.1126/sciadv.aeh1658. Epub 2026 Oct 28. PMID- 42950045 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2375-2548 (Electronic) IS - 2375-2548 (Linking) VI - 12 IP - 44 DP - 2026 Oct 30 TI - The structural history of Eukarya. PG - eaeh2524 LID - 10.1126/sciadv.aeh2524 [doi] LID - eaeh2524 AB - Comparative genomics has traditionally relied on sequence-based markers, yet the global evolutionary landscape of protein architecture remains largely unexplored. We present the Structural History of Eukarya (SHE), a structural similarity-derived phylogeny from all-vs-all structural comparisons on the proteome level of 1542 eukaryotic species, encompassing nearly 300 trillion pairwise protein alignments. This proteome-scale analysis reveals a bipartite model of eukaryotic evolution: a rigid Strict Core dominated by cytoskeletal architecture, supporting a more plastic Operational Engine centered on translational machinery. We identify lineage-specific accelerations of structural evolution in species such as birds and ants that are decoupled from proteome expansion. Finally, we demonstrate that proteome-wide structural fidelity enables the functional annotation of uncharacterized proteins and a data-driven framework for model organism selection, replacing heuristic choices with quantitative matching to human biological processes. SHE is freely accessible as an interactive portal. FAU - Daumiller, Diandra AU - Daumiller D AUID- ORCID: 0009-0009-5387-1292 AD - The Department of Molecular Biosciences, The Wenner-Gren Institute. Stockholm University, Svante Arrhenius vag 20C, 114 18 Stockholm. AD - Science for Life Laboratory, Solna 171 65, Sweden. FAU - Li, Qiuzhen AU - Li Q AUID- ORCID: 0009-0009-2913-4786 AD - The Department of Molecular Biosciences, The Wenner-Gren Institute. Stockholm University, Svante Arrhenius vag 20C, 114 18 Stockholm. AD - Science for Life Laboratory, Solna 171 65, Sweden. FAU - Bryant, Patrick AU - Bryant P AUID- ORCID: 0000-0003-3439-1866 AD - The Department of Molecular Biosciences, The Wenner-Gren Institute. Stockholm University, Svante Arrhenius vag 20C, 114 18 Stockholm. AD - Science for Life Laboratory, Solna 171 65, Sweden. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Sci Adv JT - Science advances JID - 101653440 RN - 0 (Proteome) SB - IM MH - Phylogeny MH - Animals MH - *Evolution, Molecular MH - *Eukaryota/genetics/metabolism/classification MH - *Proteome/genetics MH - Humans PMC - PMC13715714 COIS- All authors declare that they have no competing interests. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 14:03 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:03 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - aeh2524 [pii] AID - 10.1126/sciadv.aeh2524 [doi] PST - ppublish SO - Sci Adv. 2026 Oct 30;12(44):eaeh2524. doi: 10.1126/sciadv.aeh2524. Epub 2026 Oct 28. PMID- 42950042 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2375-2548 (Electronic) IS - 2375-2548 (Linking) VI - 12 IP - 44 DP - 2026 Oct 30 TI - The nonsense-mediated decay RNA-surveillance pathway facilitates the hypoxia response by C. elegans. PG - eaej4259 LID - 10.1126/sciadv.aej4259 [doi] LID - eaej4259 AB - Hypoxia (decreased O(2)) induces physiological, metabolic, and behavioral changes. The major hypoxia response pathway is conserved from nematodes to mammals and is driven by activation of the HIF (hypoxia inducible factor) family of transcription factors. Despite a fundamental role of the HIF pathway in regulating cellular homeostasis in response to hypoxic stress, interactions between the HIF pathway and cellular quality control pathways are largely unknown. Using an unbiased genetic screen, we found that in C. elegans nonsense-mediated decay (NMD), an evolutionarily conserved mechanism of RNA surveillance, acts in parallel to HIF-1 to selectively enhance specific aspects of the response to HIF-1 activation, possibly through coregulation of a distinct subset of HIF-1-up-regulated genes. Our findings reveal a functional integration between oxygen sensing and RNA surveillance and establish NMD as a key regulator of specific aspects of the HIF-1-driven transcriptional program and physiological response. FAU - Diehl, Calista AU - Diehl C AUID- ORCID: 0000-0003-2033-5732 AD - Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology Cambridge, MA 02139, USA. FAU - Horvitz, H Robert AU - Horvitz HR AUID- ORCID: 0000-0002-9964-9613 AD - Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology Cambridge, MA 02139, USA. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Sci Adv JT - Science advances JID - 101653440 RN - 0 (Caenorhabditis elegans Proteins) RN - 0 (Hypoxia-Inducible Factor 1) RN - 0 (HIF-1 protein, C elegans) RN - S88TT14065 (Oxygen) RN - 0 (Transcription Factors) SB - IM MH - Animals MH - *Caenorhabditis elegans/genetics/metabolism MH - *Nonsense Mediated mRNA Decay MH - Caenorhabditis elegans Proteins/genetics/metabolism MH - *Hypoxia/genetics/metabolism MH - Hypoxia-Inducible Factor 1/metabolism/genetics MH - Signal Transduction MH - Gene Expression Regulation MH - Oxygen/metabolism MH - Transcription Factors PMC - PMC13715696 COIS- The authors declare that they have no competing interests. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 14:03 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:03 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - aej4259 [pii] AID - 10.1126/sciadv.aej4259 [doi] PST - ppublish SO - Sci Adv. 2026 Oct 30;12(44):eaej4259. doi: 10.1126/sciadv.aej4259. Epub 2026 Oct 28. PMID- 42950040 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 2375-2548 (Electronic) IS - 2375-2548 (Linking) VI - 12 IP - 44 DP - 2026 Oct 30 TI - Momentum quantization of hyperbolic phonon polaritons in photonic-crystal cavity. PG - eaej0277 LID - 10.1126/sciadv.aej0277 [doi] LID - eaej0277 AB - Hyperbolic phonon polaritons (HPhPs) in van der Waals crystals enable extreme electromagnetic confinement and access to exceptionally large in-plane momenta, offering a powerful platform for deep-subwavelength nanophotonics. Although the lower Reststrahlen band (RS1) of hexagonal boron nitride (h-BN) is highly attractive for low-loss, strongly volume-confined polaritons, fully exploiting this potential remains challenging because RS1 modes are weakly accessible by conventional near-field detection and require efficient leakage suppression for high-Q confinement. Here, we demonstrate that momentum quantization of RS1 HPhPs enables high-Q, ultrasmall mode volume cavity states in an h-BN photonic-crystal nanocavity architecture. Periodic lateral cavity boundaries impose Fabry-Perot phase matching, while the surrounding photonic-crystal lattice suppresses in-plane leakage and stabilizes discrete cavity-supported momentum states, transforming the continuous hyperbolic dispersion into a ladder of geometry-defined momentum eigenstates. Using photo-induced force microscopy, we directly visualize the quantized cavity modes in real space and reconstruct their discretized dispersion in momentum space. The resulting hyperbolic polaritonic cavity crystal exhibits quality factors exceeding 340 and ultrasmall mode volumes down to [Formula: see text], corresponding to Purcell enhancements approaching 10(7). This approach establishes a route to high-Q, ultrasmall-volume RS1 polaritonic nanocavities with geometry-defined momentum states, providing a scalable platform for enhanced light-matter interactions, selective vibrational spectroscopy, and reconfigurable mid-infrared nanophotonic devices. FAU - Woo, Hwi Je AU - Woo HJ AUID- ORCID: 0000-0001-9630-0128 AD - Material Property Metrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea. FAU - Choi, Heejin AU - Choi H AUID- ORCID: 0009-0004-2918-7419 AD - Institute of Advanced Optics and Photonics, Hanbat National University, Daejeon 34158, Republic of Korea. AD - Department of Physics, Sejong University, Seoul 05006, Republic of Korea. FAU - Biswas, Piyali AU - Biswas P AUID- ORCID: 0000-0003-1196-932X AD - Institute of Advanced Optics and Photonics, Hanbat National University, Daejeon 34158, Republic of Korea. FAU - Kim, Seonyeong AU - Kim S AUID- ORCID: 0000-0003-2364-0003 AD - Institute of Advanced Optics and Photonics, Hanbat National University, Daejeon 34158, Republic of Korea. FAU - Kim, Hansung AU - Kim H AUID- ORCID: 0009-0003-5584-9714 AD - Material Property Metrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea. AD - Department of Physics, Sejong University, Seoul 05006, Republic of Korea. FAU - Seo, Sunae AU - Seo S AD - Department of Physics, Sejong University, Seoul 05006, Republic of Korea. FAU - Jung, Suyong AU - Jung S AUID- ORCID: 0000-0003-3885-9999 AD - Material Property Metrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea. FAU - Lee, Eun Seong AU - Lee ES AUID- ORCID: 0000-0001-9444-1824 AD - Material Property Metrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea. FAU - Jahng, Junghoon AU - Jahng J AUID- ORCID: 0000-0002-5745-9686 AD - Material Property Metrology Group, Korea Research Institute of Standards and Science (KRISS), Daejeon 34113, Republic of Korea. FAU - Lee, Chang-Won AU - Lee CW AUID- ORCID: 0000-0003-0546-0439 AD - Institute of Advanced Optics and Photonics, Hanbat National University, Daejeon 34158, Republic of Korea. AD - Department of Applied Optics, School of Basic Sciences, Hanbat National University, Daejeon 34158, Republic of Korea. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Sci Adv JT - Science advances JID - 101653440 SB - IM PMC - PMC13715691 COIS- The authors declare that they have no competing interests. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 14:03 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:03 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - aej0277 [pii] AID - 10.1126/sciadv.aej0277 [doi] PST - ppublish SO - Sci Adv. 2026 Oct 30;12(44):eaej0277. doi: 10.1126/sciadv.aej0277. Epub 2026 Oct 28. PMID- 42950035 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 2375-2548 (Electronic) IS - 2375-2548 (Linking) VI - 12 IP - 44 DP - 2026 Oct 30 TI - Engineering a closed-loop electrochemical pathway for cadmium recovery and aqueous battery reutilization. PG - eaek6073 LID - 10.1126/sciadv.aek6073 [doi] LID - eaek6073 AB - Cadmium (Cd) waste is an unavoidable toxic by-product of zinc processing, but its electrochemical reutilization remains limited by the lack of routes that couple selective recovery with stable metal-anode operation. Here, we report a closed-loop electrochemical strategy that transforms model Cd-containing waste electrolytes into regenerable aqueous Cd metal anodes. A three-step potential-programmed electrodeposition process selectively recovers Cd while constructing compact Cd(002)-textured electrodes with tunable areal capacity. To preserve this recovered structure during cycling, cesium iodide is introduced to reconstruct the Cd-electrolyte interface, enabling iodide-dominated and cesium-assisted regulation of desolvation, ion flux, corrosion, and Cd growth. The resulting Cd anode achieves 99.98% average Coulombic efficiency and stable high-rate plating/stripping. Cd||I(2) coin and pouch cells validate full-cell operation under high loading and reduced excess Cd. Failed Cd anodes can be reextracted, regenerated, and reused, completing a recovery-operation-regeneration loop. FAU - Wang, Yue AU - Wang Y AUID- ORCID: 0000-0001-6819-1374 AD - Department of Materials Science and Engineering, College of Design and Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117575, Singapore. FAU - Zhao, Nan AU - Zhao N AD - Pillar of Engineering Product Development, Singapore University of Technology and Design, 8 Somapah Road, Singapore 487372, Singapore. FAU - Song, Haobin AU - Song H AUID- ORCID: 0000-0003-3392-2557 AD - Department of Materials Science and Engineering, College of Design and Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117575, Singapore. AD - Pillar of Engineering Product Development, Singapore University of Technology and Design, 8 Somapah Road, Singapore 487372, Singapore. FAU - Cui, Yangfeng AU - Cui Y AUID- ORCID: 0000-0003-4060-7627 AD - Department of Materials Science and Engineering, College of Design and Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117575, Singapore. FAU - Yang, Hui Ying AU - Yang HY AUID- ORCID: 0000-0002-2244-8231 AD - Department of Materials Science and Engineering, College of Design and Engineering, National University of Singapore, 9 Engineering Drive 1, Singapore 117575, Singapore. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Sci Adv JT - Science advances JID - 101653440 SB - IM PMC - PMC13715700 COIS- The authors declare that they have no competing interests. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 14:03 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:03 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - aek6073 [pii] AID - 10.1126/sciadv.aek6073 [doi] PST - ppublish SO - Sci Adv. 2026 Oct 30;12(44):eaek6073. doi: 10.1126/sciadv.aek6073. Epub 2026 Oct 28. PMID- 42950032 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 2375-2548 (Electronic) IS - 2375-2548 (Linking) VI - 12 IP - 44 DP - 2026 Oct 30 TI - Promoting CH(4) photoconversion by reconstruction of polaron states. PG - eaej4630 LID - 10.1126/sciadv.aej4630 [doi] LID - eaej4630 AB - Selective oxidation of methane (CH(4)) has attractive potentials for mitigating global warming and producing value-added chemicals, while the activation of highly inert CH(4) remains challenging. Herein, we developed a single-atomic Pt-doped WO(3) (Pt(1)-WO(3)) catalyst, which enabled to reconstruct polaron states from W to Pt-O sites. This polaron reconstruction allows for fast separation of photogenerated charge carriers and tuning of reactant adsorption sites, and greatly improves the photocatalytic CH(4) activation and conversion efficiency. Under 150 mW.cm(-2) light intensity and aqueous conditions, the Pt(1)-WO(3) photocatalyst exhibited a peak CH(4)-to-CH(3)OH conversion yield of 1151 mumol.g(cat)(-1).h(-1) without the use of photosensitizers, sacrificial reagents or strong oxidizers like O(2) or H(2)O(2), exceeding the previous reports on photocatalytic CH(4) oxidation under similar conditions, thus suggesting the capability of tuning polaron states for enhanced photocatalysis. FAU - Hao, Shuya AU - Hao S AUID- ORCID: 0009-0009-7754-1490 AD - Laboratory of Advanced Materials, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Shanghai Academy of Natural Sciences; Fudan University, Shanghai 200438, China. FAU - Xue, Yuanyuan AU - Xue Y AUID- ORCID: 0000-0002-1639-6316 AD - Laboratory of Advanced Materials, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Shanghai Academy of Natural Sciences; Fudan University, Shanghai 200438, China. FAU - Lv, Ximeng AU - Lv X AD - Laboratory of Advanced Materials, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Shanghai Academy of Natural Sciences; Fudan University, Shanghai 200438, China. FAU - Peng, Chen AU - Peng C AUID- ORCID: 0000-0003-2767-7698 AD - Laboratory of Advanced Materials, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Shanghai Academy of Natural Sciences; Fudan University, Shanghai 200438, China. FAU - Wei, Ruilin AU - Wei R AD - Laboratory of Advanced Materials, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Shanghai Academy of Natural Sciences; Fudan University, Shanghai 200438, China. FAU - Wang, Huining AU - Wang H AD - Laboratory of Advanced Materials, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Shanghai Academy of Natural Sciences; Fudan University, Shanghai 200438, China. FAU - Han, Qing AU - Han Q AD - Laboratory of Advanced Materials, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Shanghai Academy of Natural Sciences; Fudan University, Shanghai 200438, China. FAU - Zheng, Gengfeng AU - Zheng G AUID- ORCID: 0000-0002-1803-6955 AD - Laboratory of Advanced Materials, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Shanghai Academy of Natural Sciences; Fudan University, Shanghai 200438, China. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Sci Adv JT - Science advances JID - 101653440 SB - IM PMC - PMC13715711 COIS- The authors declare they have no competing interests. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 14:03 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:03 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - aej4630 [pii] AID - 10.1126/sciadv.aej4630 [doi] PST - ppublish SO - Sci Adv. 2026 Oct 30;12(44):eaej4630. doi: 10.1126/sciadv.aej4630. Epub 2026 Oct 28. PMID- 42950029 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2375-2548 (Electronic) IS - 2375-2548 (Linking) VI - 12 IP - 44 DP - 2026 Oct 30 TI - Nanomaterial-genetics enables near-infrared-driven genetically targeted neuromodulation. PG - eaef7843 LID - 10.1126/sciadv.aef7843 [doi] LID - eaef7843 AB - Stimuli-responsive nanomaterials offer powerful capabilities for neuromodulation, yet there is a continuous need for more versatile methods to ensure cell-specific targeting, a prerequisite for their precise application in neuroscience. To address this gap, we introduce the nanomaterial-genetics (NMG) system, a strategy that integrates genetically encoded synthetic receptors with ligand-functionalized nanoparticles to achieve receptor-guided, cell-selective targeting. Neurons are engineered to express an NMG Receptor carrying an extracellular HaloTag, which selectively captures HaloTag ligand-functionalized gold nanoparticles (AuNP-Ligands). This genetically encoded receptor-ligand pairing enables efficient and localized nanomaterial-mediated photothermal activation under near-infrared (NIR) irradiation at ultralow nanoparticle doses, whereas receptor-negative neurons remain largely unresponsive. Receptor-guided targeting substantially reduces nanomaterial-associated cytotoxicity under the tested conditions while preserving robust neuromodulatory efficacy. We demonstrate genetically targeted activation of neurons in vitro and in vivo, including transcranial NIR modulation of mouse locomotor behavior. Collectively, the NMG system offers an extensible complement to current neuromodulation technologies, providing a versatile platform for multimodal and precision neuromodulation. FAU - Liu, Yi AU - Liu Y AUID- ORCID: 0000-0002-0088-5203 AD - Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education; Key Laboratory of Innovation and Transformation of Advanced Medical Devices, Ministry of Industry and Information Technology; National Medical Innovation Platform for Industry-Education Integration in Advanced Medical Devices (Interdiscipline of Medicine and Engineering); School of Biological Science and Medical Engineering, School of Engineering Medicine, Beihang University, Beijing 100191, China. AD - Zhongshan School of Medicine, Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen 518107, China. FAU - Wang, Xiaolei AU - Wang X AUID- ORCID: 0009-0005-2392-2926 AD - Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education; Key Laboratory of Innovation and Transformation of Advanced Medical Devices, Ministry of Industry and Information Technology; National Medical Innovation Platform for Industry-Education Integration in Advanced Medical Devices (Interdiscipline of Medicine and Engineering); School of Biological Science and Medical Engineering, School of Engineering Medicine, Beihang University, Beijing 100191, China. FAU - Yin, Weiwei AU - Yin W AD - IDG/McGovern Institute for Brain Research, School of Basic Medical Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China. FAU - Chen, Jiahui AU - Chen J AD - Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education; Key Laboratory of Innovation and Transformation of Advanced Medical Devices, Ministry of Industry and Information Technology; National Medical Innovation Platform for Industry-Education Integration in Advanced Medical Devices (Interdiscipline of Medicine and Engineering); School of Biological Science and Medical Engineering, School of Engineering Medicine, Beihang University, Beijing 100191, China. FAU - Fan, Yu AU - Fan Y AD - Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education; Key Laboratory of Innovation and Transformation of Advanced Medical Devices, Ministry of Industry and Information Technology; National Medical Innovation Platform for Industry-Education Integration in Advanced Medical Devices (Interdiscipline of Medicine and Engineering); School of Biological Science and Medical Engineering, School of Engineering Medicine, Beihang University, Beijing 100191, China. FAU - Yang, Jiani AU - Yang J AD - Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education; Key Laboratory of Innovation and Transformation of Advanced Medical Devices, Ministry of Industry and Information Technology; National Medical Innovation Platform for Industry-Education Integration in Advanced Medical Devices (Interdiscipline of Medicine and Engineering); School of Biological Science and Medical Engineering, School of Engineering Medicine, Beihang University, Beijing 100191, China. FAU - Wang, Yu AU - Wang Y AD - Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education; Key Laboratory of Innovation and Transformation of Advanced Medical Devices, Ministry of Industry and Information Technology; National Medical Innovation Platform for Industry-Education Integration in Advanced Medical Devices (Interdiscipline of Medicine and Engineering); School of Biological Science and Medical Engineering, School of Engineering Medicine, Beihang University, Beijing 100191, China. AD - Zhongshan School of Medicine, Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen 518107, China. FAU - Ge, Lin AU - Ge L AUID- ORCID: 0000-0002-4430-9304 AD - Neuropharmacology Platform of the Center of Pharmaceutical Technology, Tsinghua University, Beijing 100084, China. FAU - Rao, Wei AU - Rao W AUID- ORCID: 0000-0002-4168-0298 AD - State Key Laboratory of Cryogenic Science and Technology and Beijing Key Laboratory of Cryobiomedicine, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China. FAU - Wang, Laiyuan AU - Wang L AD - School of Flexible Electronics (SoFE) and State Key Laboratory of Optoelectronic Materials and Technologies (OEMT), Sun Yat-sen University, Shenzhen 518107, China. FAU - Li, Cheng AU - Li C AD - Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education; Key Laboratory of Innovation and Transformation of Advanced Medical Devices, Ministry of Industry and Information Technology; National Medical Innovation Platform for Industry-Education Integration in Advanced Medical Devices (Interdiscipline of Medicine and Engineering); School of Biological Science and Medical Engineering, School of Engineering Medicine, Beihang University, Beijing 100191, China. FAU - Guo, Zengcai V AU - Guo ZV AD - IDG/McGovern Institute for Brain Research, School of Basic Medical Sciences, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing 100084, China. FAU - Liu, Nan AU - Liu N AUID- ORCID: 0000-0002-9606-4732 AD - School of Life Sciences, Yunnan University, Kunming 650091, China. FAU - Sun, Xuyang AU - Sun X AUID- ORCID: 0000-0003-0867-1100 AD - Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education; Key Laboratory of Innovation and Transformation of Advanced Medical Devices, Ministry of Industry and Information Technology; National Medical Innovation Platform for Industry-Education Integration in Advanced Medical Devices (Interdiscipline of Medicine and Engineering); School of Biological Science and Medical Engineering, School of Engineering Medicine, Beihang University, Beijing 100191, China. FAU - Yang, Yaxiong AU - Yang Y AUID- ORCID: 0000-0002-3313-6049 AD - Zhongshan School of Medicine, Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen 518107, China. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Sci Adv JT - Science advances JID - 101653440 RN - 7440-57-5 (Gold) RN - 0 (Ligands) SB - IM MH - Animals MH - *Neurons/metabolism MH - *Infrared Rays MH - Mice MH - Gold/chemistry MH - Metal Nanoparticles/chemistry MH - *Nanostructures/chemistry MH - Humans MH - Ligands PMC - PMC13715694 COIS- The authors declare that they have no competing interests. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 14:03 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:03 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - aef7843 [pii] AID - 10.1126/sciadv.aef7843 [doi] PST - ppublish SO - Sci Adv. 2026 Oct 30;12(44):eaef7843. doi: 10.1126/sciadv.aef7843. Epub 2026 Oct 28. PMID- 42950026 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2375-2548 (Electronic) IS - 2375-2548 (Linking) VI - 12 IP - 44 DP - 2026 Oct 30 TI - FOSL1-associated condensates modulate superenhancer-mediated selected IL-1 family gene activation in psoriasis. PG - eaeh6541 LID - 10.1126/sciadv.aeh6541 [doi] LID - eaeh6541 AB - Psoriasis is a chronic inflammatory skin disease involving abnormal keratinocyte gene expression, yet the upstream epigenetic mechanisms driving these alterations remain unclear. Here we performed multiomics analyses (chromatin immunoprecipitation sequencing, CUT&Tag, ATAC-seq, and RNA sequencing) on psoriatic lesions, perilesional skin, healthy skin, and M5-stimulated HaCaT cells. Functional validation was performed using CRISPR-mediated superenhancer (SE) editing, epigenetic modulation, circular chromosome conformation capture sequencing, assay for chromatin-bound condensates by exploratory sequencing, coimmunoprecipitation, and fluorescence recovery after photobleaching. We found widespread activation of enhancers and SEs in lesions, including an interleukin 1 (IL-1)-SE regulating IL1A, IL1B, and IL36G transcription. BET inhibition reduced SE activity, repressed IL-1 cytokines, and relieved psoriasis-like inflammation in vivo. Fos-like antigen 1 (FOSL1) was identified as a key transcription factor enriched in active SEs and associated with condensate formation, contributing to the maintenance of EP300 occupancy and histone H3 lysine 27 acetylation deposition. Macrophage-derived IL-1 up-regulated FOSL1 through mitogen-activated protein kinase/ERK signaling, forming a positive feedback loop that amplified inflammation. This study revealed an FOSL1-SE-centered regulatory network driving keratinocyte inflammatory responses, providing previously unknown therapeutic targets for psoriasis. FAU - Qiu, Yueqi AU - Qiu Y AUID- ORCID: 0000-0003-2467-2701 AD - Hospital for Skin Diseases, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China. AD - Key Laboratory of Basic and Translational Research on Immune-Mediated Skin Diseases, Chinese Academy of Medical Sciences, Nanjing, China. AD - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Nanjing, China. FAU - Sun, Ke AU - Sun K AUID- ORCID: 0009-0005-1777-0719 AD - Hospital for Skin Diseases, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China. AD - Key Laboratory of Basic and Translational Research on Immune-Mediated Skin Diseases, Chinese Academy of Medical Sciences, Nanjing, China. FAU - Yu, Yaqin AU - Yu Y AD - Key Laboratory of Basic and Translational Research on Immune-Mediated Skin Diseases, Chinese Academy of Medical Sciences, Nanjing, China. AD - Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital, Central South University, Changsha, China. FAU - Hou, Huihui AU - Hou H AUID- ORCID: 0009-0003-2634-2703 AD - Key Laboratory of Basic and Translational Research on Immune-Mediated Skin Diseases, Chinese Academy of Medical Sciences, Nanjing, China. AD - School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, China. FAU - Wu, Ruifang AU - Wu R AUID- ORCID: 0000-0001-9923-949X AD - Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital, Central South University, Changsha, China. FAU - Lu, Qianjin AU - Lu Q AUID- ORCID: 0009-0009-3625-0603 AD - Hospital for Skin Diseases, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China. AD - Key Laboratory of Basic and Translational Research on Immune-Mediated Skin Diseases, Chinese Academy of Medical Sciences, Nanjing, China. AD - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Nanjing, China. AD - Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital, Central South University, Changsha, China. FAU - Zhao, Ming AU - Zhao M AUID- ORCID: 0000-0002-1320-1093 AD - Hospital for Skin Diseases, Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, China. AD - Key Laboratory of Basic and Translational Research on Immune-Mediated Skin Diseases, Chinese Academy of Medical Sciences, Nanjing, China. AD - Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Nanjing, China. AD - Hunan Key Laboratory of Medical Epigenomics, The Second Xiangya Hospital, Central South University, Changsha, China. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Sci Adv JT - Science advances JID - 101653440 RN - 0 (Proto-Oncogene Proteins c-fos) RN - 0 (Fos-Related Antigen 1) RN - 0 (Interleukin-1) SB - IM MH - Humans MH - *Psoriasis/genetics/metabolism/pathology MH - *Proto-Oncogene Proteins c-fos/metabolism/genetics MH - Fos-Related Antigen 1 MH - *Interleukin-1/genetics/metabolism MH - Keratinocytes/metabolism MH - *Enhancer Elements, Genetic MH - Animals MH - Gene Expression Regulation MH - Epigenesis, Genetic MH - *Transcriptional Activation MH - HaCaT Cells PMC - PMC13715713 COIS- The authors declare that they have no competing interests. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 14:03 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:03 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - aeh6541 [pii] AID - 10.1126/sciadv.aeh6541 [doi] PST - ppublish SO - Sci Adv. 2026 Oct 30;12(44):eaeh6541. doi: 10.1126/sciadv.aeh6541. Epub 2026 Oct 28. PMID- 42950025 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 2375-2548 (Electronic) IS - 2375-2548 (Linking) VI - 12 IP - 44 DP - 2026 Oct 30 TI - Visible-light-mediated stereoselective construction of oxa-spiro bicyclohexanes via strain-release [2pi + 2sigma] cycloaddition. PG - eaeh7873 LID - 10.1126/sciadv.aeh7873 [doi] LID - eaeh7873 AB - The stereoselective construction of rigid, three-dimensional architectures from compact building blocks remains a central challenge in organic synthesis. Here, we report a visible-light-mediated strain-release [2pi + 2sigma] spiroannulation of cyclopentene-1,3-diones (CPDs) with bicyclo[1.1.0]butanes (BCBs), giving efficient access to underexplored oxa-spiro-BCH frameworks under mild conditions. The reaction forms two bonds with excellent regioselectivity and diastereoselectivity (>20:1 dr), with stereocontrol extending to a center formed remotely from the initial bond-forming event. Mechanistic experiments and DFT calculations support excitation of the CPD to a triplet (n,pi*) state, selective oxygen-centered capture of the strained BCB sigma bond, intersystem crossing, and diastereodifferentiating radical recombination, the selectivity arising from a stabilizing pi-pi interaction in the recombination transition state. These findings uncover an unusual mode of CPD triplet-state reactivity while providing stereoselective access to conformationally constrained, sp(3)-rich frameworks whose substituent-vector geometry shows an unexpected correspondence to that of 1,7-disubstituted naphthalene. FAU - Singh, Sanjay AU - Singh S AUID- ORCID: 0000-0003-1244-5675 AD - Center for Catalytic Hydrocarbon Functionalizations, Institute for Basic Science (IBS), Daejeon 34141, Korea. AD - Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea. AD - Department of Chemistry, Pandit Deendayal Energy University, Gandhinagar, Gujarat 382426, India. FAU - Kim, Changha AU - Kim C AUID- ORCID: 0000-0003-0119-0056 AD - Center for Catalytic Hydrocarbon Functionalizations, Institute for Basic Science (IBS), Daejeon 34141, Korea. AD - Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea. FAU - Lee, Dongwoo AU - Lee D AUID- ORCID: 0009-0008-1092-8626 AD - Center for Catalytic Hydrocarbon Functionalizations, Institute for Basic Science (IBS), Daejeon 34141, Korea. AD - Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea. FAU - Kim, Dongwook AU - Kim D AUID- ORCID: 0000-0003-4432-371X AD - Center for Catalytic Hydrocarbon Functionalizations, Institute for Basic Science (IBS), Daejeon 34141, Korea. FAU - Hong, Sungwoo AU - Hong S AUID- ORCID: 0000-0001-9371-1730 AD - Center for Catalytic Hydrocarbon Functionalizations, Institute for Basic Science (IBS), Daejeon 34141, Korea. AD - Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Sci Adv JT - Science advances JID - 101653440 SB - IM PMC - PMC13715710 COIS- The authors declare that they have no competing interests. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 14:03 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:03 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - aeh7873 [pii] AID - 10.1126/sciadv.aeh7873 [doi] PST - ppublish SO - Sci Adv. 2026 Oct 30;12(44):eaeh7873. doi: 10.1126/sciadv.aeh7873. Epub 2026 Oct 28. PMID- 42950019 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1946-6242 (Electronic) IS - 1946-6234 (Linking) VI - 18 IP - 873 DP - 2026 Oct 28 TI - T cell-mediated depletion of intratumoral regulatory T cells using a CCR8xCD3 T cell-engaging bispecific antibody (TCB) promotes antitumor immunity in preclinical mouse models. PG - eaea7127 LID - 10.1126/scitranslmed.aea7127 [doi] AB - T cell-engaging bispecific antibodies (TCBs) have seen notable successes in hematologic malignancies, and emerging data demonstrate their potential for the treatment of solid tumors. Most oncology applications of TCBs have focused on targeting tumor cells via tumor-specific antigens; however, TCBs have been underutilized for strategic depletion of immunosuppressive cells in the TME. Regulatory T cells (T(reg) cells) play a crucial role in suppressing antitumor immune responses, making them an attractive target for immune-based therapies. T(reg) cells express several surface markers that can serve as potential targets for antibody-mediated depletion, including C-C chemokine receptor type 8 (CCR8), which is highly enriched on tumor-infiltrating T(reg) cells. Monoclonal antibody (mAb)-mediated T(reg) cell depletion relies on natural killer (NK) cells, which can be scarce in some solid tumors, whereas TCBs can broadly engage the T cell compartment by overwriting antigen specificity and redirecting potent T cell cytotoxicity against target cells. Here, we have developed a CCR8xCD3 TCB and show that it effectively depletes CCR8-expressing T(reg) cells in a T cell-mediated manner. CCR8xCD3 TCB led to the selective depletion of intratumoral T(reg) cells from human solid tumor samples in vitro and mediated inhibition of tumor growth in multiple syngeneic tumor models with variable levels of T cell infiltration alone or in combination with anti-PD-L1. In cynomolgus monkeys, CCR8xCD3 TCB was well tolerated without evidence of acute phase inflammation or cytokine release syndrome and led to effective and durable depletion of the CCR8-expressing subset of T(reg) cells. FAU - Wang, Baomei AU - Wang B AUID- ORCID: 0000-0002-0662-600X AD - Department of Research Oncology, Genentech, South San Francisco, CA, USA. FAU - Ishizuka, Isabel AU - Ishizuka I AUID- ORCID: 0000-0002-0758-8076 AD - Department of Research Oncology, Genentech, South San Francisco, CA, USA. FAU - Esen, Emel AU - Esen E AUID- ORCID: 0000-0002-3144-0346 AD - Department of Translational Medicine, Genentech, South San Francisco, CA, USA. FAU - Li, Ji AU - Li J AUID- ORCID: 0000-0002-1481-4289 AD - Department of Research Oncology, Genentech, South San Francisco, CA, USA. FAU - Chiu, Cecilia AU - Chiu C AUID- ORCID: 0000-0001-8797-9984 AD - Department of Antibody Engineering, Genentech, South San Francisco, CA, USA. FAU - Sun, Yonglian AU - Sun Y AUID- ORCID: 0000-0001-7622-6821 AD - Department of Antibody Engineering, Genentech, South San Francisco, CA, USA. FAU - Lin, Zhonghua AU - Lin Z AUID- ORCID: 0000-0002-8397-5780 AD - Department of Antibody Engineering, Genentech, South San Francisco, CA, USA. FAU - Seshasayee, Dhaya AU - Seshasayee D AUID- ORCID: 0000-0002-8430-3433 AD - Department of Antibody Engineering, Genentech, South San Francisco, CA, USA. FAU - Kusi, Aija AU - Kusi A AUID- ORCID: 0009-0004-0044-2746 AD - Department of Developmental Sciences Translational Safety, Genentech, South San Francisco, CA, USA. FAU - Zhong, Yu AU - Zhong Y AD - Department of Developmental Sciences Translational Safety, Genentech, South San Francisco, CA, USA. FAU - Halpern, Wendy AU - Halpern W AUID- ORCID: 0000-0002-5060-6874 AD - Department of Developmental Sciences Translational Safety, Genentech, South San Francisco, CA, USA. FAU - Adedeji, Adeyemi O AU - Adedeji AO AD - Department of Developmental Sciences Translational Safety, Genentech, South San Francisco, CA, USA. FAU - Yu, Lanlan AU - Yu L AD - Department of Developmental Sciences Translational Safety, Genentech, South San Francisco, CA, USA. FAU - Tran, Peter AU - Tran P AD - Department of Developmental Sciences BioAnalytical Sciences, Genentech, South San Francisco, CA, USA. FAU - Yin, Zhaojun AU - Yin Z AUID- ORCID: 0009-0009-4610-9486 AD - Department of Developmental Sciences BioAnalytical Sciences, Genentech, South San Francisco, CA, USA. FAU - Yu, Shang-Fan AU - Yu SF AD - Department of Translational Oncology, Genentech, South San Francisco, CA, USA. FAU - Del Rosario, Geoff AU - Del Rosario G AD - Department of Translational Oncology, Genentech, South San Francisco, CA, USA. FAU - Oei, Yoko AU - Oei Y AD - Department of Translational Oncology, Genentech, South San Francisco, CA, USA. FAU - Yang, Yagai AU - Yang Y AD - Department of Translational Oncology, Genentech, South San Francisco, CA, USA. FAU - Schartner, Jill AU - Schartner J AD - Department of Translational Oncology, Genentech, South San Francisco, CA, USA. FAU - Go, Mary Ann AU - Go MA AD - Department of Translational Oncology, Genentech, South San Francisco, CA, USA. FAU - Protti, Giulia AU - Protti G AUID- ORCID: 0000-0002-7144-3285 AD - Department of Computational Biology & Medicine, Genentech, South San Francisco, CA, USA. FAU - Muller, Soren AU - Muller S AUID- ORCID: 0000-0001-9490-5270 AD - Department of Computational Biology & Medicine, Genentech, South San Francisco, CA, USA. FAU - Wu, Cong AU - Wu C AD - Department of Biochemical Cellular Pharmacology, Genentech, South San Francisco, CA, USA. FAU - Li, Ran AU - Li R AUID- ORCID: 0000-0002-8537-8824 AD - Department of Translational Pharmacokinetics and Pharmacodynamics Developmental Sciences PTPK, Development Science, Genentech, South San Francisco, CA, USA. FAU - Lee, Genee AU - Lee G AUID- ORCID: 0000-0002-8835-4297 AD - Department of Research Oncology, Genentech, South San Francisco, CA, USA. FAU - Koerber, James T AU - Koerber JT AUID- ORCID: 0000-0001-7756-972X AD - Department of Antibody Engineering, Genentech, South San Francisco, CA, USA. FAU - Rutz, Sascha AU - Rutz S AUID- ORCID: 0009-0004-2853-882X AD - Department of Research Oncology, Genentech, South San Francisco, CA, USA. FAU - Junttila, Teemu T AU - Junttila TT AUID- ORCID: 0000-0001-9936-2420 AD - Department of Research Oncology, Genentech, South San Francisco, CA, USA. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Sci Transl Med JT - Science translational medicine JID - 101505086 RN - 0 (Antibodies, Bispecific) RN - 0 (Receptors, CCR8) RN - 0 (CD3 Complex) SB - IM MH - Animals MH - *T-Lymphocytes, Regulatory/immunology MH - *Antibodies, Bispecific/immunology/pharmacology MH - Humans MH - *Receptors, CCR8/metabolism/immunology MH - Cell Line, Tumor MH - *Lymphocyte Depletion MH - *CD3 Complex/metabolism/immunology MH - Disease Models, Animal MH - Mice MH - Female MH - *Neoplasms/immunology MH - Macaca fascicularis EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 CRDT- 2026/10/28 14:03 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:03 [entrez] AID - 10.1126/scitranslmed.aea7127 [doi] PST - ppublish SO - Sci Transl Med. 2026 Oct 28;18(873):eaea7127. doi: 10.1126/scitranslmed.aea7127. Epub 2026 Oct 28. PMID- 42950010 OWN - NLM STAT- Publisher LR - 20261028 IS - 2379-3694 (Electronic) IS - 2379-3694 (Linking) DP - 2026 Oct 28 TI - In Situ Analysis of UVB-Induced Keratinocyte-Melanoma Cell Communication Integrated with Scanning Electrochemical Microscopy and Microfluidic Devices. LID - 10.1021/acssensors.6c00702 [doi] AB - Ultraviolet B (UVB) radiation is a key environmental risk factor for remodeling the melanoma microenvironment, yet the bidirectional communication mechanisms between keratinocytes and melanoma cells remain incompletely understood. This study developed an in situ monitoring platform integrated with scanning electrochemical microscopy (SECM) and a microfluidic chip. With a fabricated Ca2+ ion-selective electrode as a SECM tip (Ca2+-ISE SECM tip), together with a lactate oxidase-modified Pt microelectrode tip (LOx-muPt tip) and a multi-potential step waveform (MPSW) method, the dynamic monitoring of Ca2+, lactate, and ROS in keratinocyte-melanoma cell communication has been achieved. UVB radiation dose-dependently enhanced A-375 melanoma cell migration in both monoculture and co-culture systems. Mechanistically, UVB induced ROS production and stimulated glycolysis in A-375 cells, resulting in elevated lactate release and autocrine-driven migration enhancement. Notably, in the co-culture system, this pro-migratory effect was significantly amplified, while inhibition by the glycolysis inhibitor 2-deoxy-d-glucose (2-DG) was minimal, suggesting a dominant paracrine signaling mechanism. In situ SECM monitoring further demonstrated that UVB also induced ROS generation, transient Ca2+ release, and significantly increased TGF-beta1 secretion in HaCaT keratinocytes. N-acetylcysteine (NAC) treatment blocked Ca2+ release and TGF-beta1 secretion in HaCaT cells, thereby inhibiting co-cultured A-375 cell migration. Further studies confirmed that HaCaT-derived TGF-beta1 serves as a key paracrine factor, promoting migration by upregulating YB-1 expression in A-375 cells, a process dependent on extracellular Ca2+. Consequently, this work reveals an intercellular signaling axis in the UVB-exposed skin tumor microenvironment: UVB --> ROS --> Ca2+ release --> TGF-beta1 secretion --> YB-1 activation, providing new mechanistic insights and potential therapeutic targets for microenvironment-dependent melanoma progression. CI - (c) 2026 American Chemical Society. FAU - Tu, Feng AU - Tu F AD - School of Chemistry and Molecular Engineering, East China Normal University, Shanghai200241, P. R. China. FAU - Chen, Shiqi AU - Chen S AD - School of Chemistry and Molecular Engineering, East China Normal University, Shanghai200241, P. R. China. FAU - Lu, Yuqi AU - Lu Y AD - School of Chemistry and Molecular Engineering, East China Normal University, Shanghai200241, P. R. China. FAU - Ge, Yuxi AU - Ge Y AD - School of Chemistry and Molecular Engineering, East China Normal University, Shanghai200241, P. R. China. FAU - Zhang, Fan AU - Zhang F AUID- ORCID: 0000-0003-4229-3916 AD - School of Chemistry and Molecular Engineering, East China Normal University, Shanghai200241, P. R. China. FAU - Wang, Qingjiang AU - Wang Q AUID- ORCID: 0000-0003-0767-7893 AD - School of Chemistry and Molecular Engineering, East China Normal University, Shanghai200241, P. R. China. LA - eng GR - 22074042/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261028 PL - United States TA - ACS Sens JT - ACS sensors JID - 101669031 SB - IM OTO - NOTNLM OT - all-solid-state calcium-selective microelectrode OT - cell migration OT - melanoma cells OT - microfluidic chip OT - scanning electrochemical microscopy (SECM) EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 14:03 PHST- 2026/02/14 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 14:03 [entrez] AID - 5460357 [pii] AID - 10.1021/acssensors.6c00702 [doi] PST - aheadofprint SO - ACS Sens. 2026 Oct 28. doi: 10.1021/acssensors.6c00702. PMID- 42950002 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261030 IS - 2050-084X (Electronic) IS - 2050-084X (Linking) VI - 13 DP - 2026 Oct 28 TI - Ultrasound neuromodulation hypotheses on the directionality of effects and community resource. LID - 10.7554/eLife.100827 [doi] LID - RP100827 AB - Low-intensity transcranial ultrasound stimulation (TUS) is a promising non-invasive technique for brain stimulation and focal neuromodulation. Research with humans and animal models has raised the possibility that TUS can be biased towards enhancing or suppressing neural function. Here, we first collate a set of hypotheses on the directionality of TUS effects and conduct an initial meta-analysis on available healthy human participant TUS studies reporting stimulation parameters and outcomes (n=47 studies, 52 experiments). In initial exploratory analyses with univariate tests, we find that parameters, such as the intensity and continuity of stimulation (duty cycle, DC) show statistical trends towards likely TUS neural enhancement or suppression of function. Machine-learning analyses were limited by the currently small sample size. Given that human TUS sample sizes are expected to increase, predictability on the directionality of TUS effects could improve if a database is available. Therefore, we establish an inTUS database and resource for the systematic reporting of TUS parameters and outcomes to assist in greater precision in TUS use for brain stimulation and neuromodulation. The paper concludes with a selective review of human clinical TUS studies illustrating how hypotheses on the directionality of TUS effects could be developed for empirical testing in the intended clinical application, not limited to the examples provided. CI - (c) 2024, Caffaratti et al. FAU - Caffaratti, Hugo AU - Caffaratti H AUID- ORCID: 0009-0008-4079-3910 AD - Department of Neurosurgery, University of Iowa, Iowa City, Iowa, United States. FAU - Slater, Ben AU - Slater B AD - Biosciences Institute, Newcastle University Medical School, Newcastle upon Tyne, United Kingdom. FAU - Shaheen, Nour AU - Shaheen N AD - Department of Neurosurgery, University of Iowa, Iowa City, Iowa, United States. FAU - Rhone, Ariane E AU - Rhone AE AUID- ORCID: 0000-0002-5699-8733 AD - Department of Neurosurgery, University of Iowa, Iowa City, Iowa, United States. FAU - Calmus, Ryan AU - Calmus R AD - Department of Neurosurgery, University of Iowa, Iowa City, Iowa, United States. AD - Biosciences Institute, Newcastle University Medical School, Newcastle upon Tyne, United Kingdom. FAU - Kritikos, Michael AU - Kritikos M AD - Department of Neurosurgery, University of Iowa, Iowa City, Iowa, United States. FAU - Kumar, Sukhbinder AU - Kumar S AD - Department of Neurosurgery, University of Iowa, Iowa City, Iowa, United States. FAU - Dlouhy, Brian AU - Dlouhy B AD - Department of Neurosurgery, University of Iowa, Iowa City, Iowa, United States. FAU - Oya, Hiroyuki AU - Oya H AUID- ORCID: 0000-0002-1733-5478 AD - Department of Neurosurgery, University of Iowa, Iowa City, Iowa, United States. FAU - Griffiths, Timothy D AU - Griffiths TD AUID- ORCID: 0000-0001-8066-4381 AD - Department of Neurosurgery, University of Iowa, Iowa City, Iowa, United States. AD - Biosciences Institute, Newcastle University Medical School, Newcastle upon Tyne, United Kingdom. FAU - Boes, Aaron AU - Boes A AD - Department of Psychiatry, University of Iowa, Iowa City, United States. FAU - Trapp, Nicholas AU - Trapp N AD - Department of Psychiatry, University of Iowa, Iowa City, United States. FAU - Kaiser, Marcus AU - Kaiser M AD - NIHR Biomedical Research Centre, School of Medicine, University of Nottingham, Nottingham, United Kingdom. AD - Rui Jin Hospital, Shanghai Jiao Tong University, Shanghai, China. FAU - Sallet, Jerome AU - Sallet J AD - Stem Cell and Brain Research Institute, INSERM U1208, University of Lyon, Lyon, France. AD - Department of Experimental Psychology, University of Oxford, Oxford, United Kingdom. FAU - Banks, Matthew I AU - Banks MI AD - Department of Anesthesiology, University of Wisconsin at Madison, Madison, United States. FAU - Howard, Matthew A 3rd AU - Howard MA 3rd AD - Department of Neurosurgery, University of Iowa, Iowa City, Iowa, United States. FAU - Zanaty, Mario AU - Zanaty M AD - Department of Neurosurgery, University of Iowa, Iowa City, Iowa, United States. FAU - Petkov, Chris AU - Petkov C AUID- ORCID: 0000-0002-4932-7907 AD - Department of Neurosurgery, University of Iowa, Iowa City, Iowa, United States. AD - Biosciences Institute, Newcastle University Medical School, Newcastle upon Tyne, United Kingdom. AD - Iowa Neuroscience Institute, University of Iowa, Iowa City, United States. LA - eng GR - U01NS137991/NH/NIH HHS/United States GR - SBE-2342847/National Science Foundation/ GR - R01DC004290/NH/NIH HHS/United States GR - PhD studentship/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom GR - EP/W004488/1/Engineering and Physical Sciences Research Council/ GR - EP/X01925X/1/Engineering and Physical Sciences Research Council/ GR - UKRI527/MRC_/Medical Research Council/United Kingdom GR - Guangci Professorship Program/Ruijin Hospital/ PT - Journal Article PT - Meta-Analysis DEP - 20261028 PL - England TA - Elife JT - eLife JID - 101579614 SB - IM UOF - medRxiv. 2025 Jul 08:2024.06.14.24308829. doi: 10.1101/2024.06.14.24308829. PMID: 38947047 MH - Humans MH - *Brain/physiology/radiation effects PMC - PMC13716386 OTO - NOTNLM OT - Human OT - brain stimulation OT - human OT - low intensity focused ultrasound OT - neuromodulation OT - neuroscience OT - non-invasive OT - transcranial ultrasound COIS- HC, BS, NS, AR, RC, MK, SK, BD, HO, TG, AB, NT, MK, JS, MB, MH, MZ, CP No competing interests declared EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 13:53 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:53 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 100827 [pii] AID - 10.7554/eLife.100827 [doi] PST - epublish SO - Elife. 2026 Oct 28;13:RP100827. doi: 10.7554/eLife.100827. PMID- 42949996 OWN - NLM STAT- Publisher LR - 20261028 IS - 1553-7358 (Electronic) IS - 1553-734X (Linking) VI - 22 IP - 10 DP - 2026 Oct 28 TI - The evolutionary advantage of replacers in the Moran process. PG - e1014892 LID - 10.1371/journal.pcbi.1014892 [doi] AB - Evolution occurs in populations of reproducing individuals. In stochastic descriptions of evolutionary dynamics, such as the Moran process, individuals are chosen randomly for birth and for death. If the same type is chosen for both steps, then the reproductive event is wasted, because the composition of the population remains unchanged. Here we introduce a new phenotype, which we call a replacer. Replacers are efficient competitors. When a replacer is chosen for reproduction, the offspring will always replace an individual of another type (if available). We determine the selective advantage of replacers in well-mixed populations and on one-dimensional lattices. We find that being a replacer substantially boosts the fixation probability of neutral and deleterious mutants. In particular, fixation probability of a single neutral replacer who invades a well-mixed population of size N is of the order of 1/N rather than the standard 1/N. Even more importantly, replacers are much better protected against invasions once they have reached fixation. Therefore, replacers dominate the mutation selection equilibrium even if the phenotype of being a replacer comes at a substantial cost: curiously, for large population sizes and small mutation rates, the relative reproductive rate of a successful replacer can be as low as 1/e. CI - Copyright: (c) 2026 Pecho et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Pecho, Michal AU - Pecho M AUID- ORCID: 0009-0007-2852-3080 AD - Computer Science Institute, Charles University, Prague, Czech Republic. FAU - Tkadlec, Josef AU - Tkadlec J AUID- ORCID: 0000-0002-1097-9684 AD - Computer Science Institute, Charles University, Prague, Czech Republic. FAU - Nowak, Martin A AU - Nowak MA AD - Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, Massachusetts, United States of America. AD - Department of Mathematics, Harvard University, Cambridge, Massachusetts, United States of America. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - PLoS Comput Biol JT - PLoS computational biology JID - 101238922 SB - IM COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 13:53 PHST- 2026/06/10 00:00 [received] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:53 [entrez] AID - PCOMPBIOL-D-26-01414 [pii] AID - 10.1371/journal.pcbi.1014892 [doi] PST - aheadofprint SO - PLoS Comput Biol. 2026 Oct 28;22(10):e1014892. doi: 10.1371/journal.pcbi.1014892. PMID- 42949992 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Farming system dynamics and sustainability pathways in three districts of Jimma zone, Southwestern Ethiopia. PG - e0359541 LID - 10.1371/journal.pone.0359541 [doi] LID - e0359541 AB - Smallholder farmers in southwestern Ethiopia face overlapping challenges that trap them in cycles of poverty and land degradation, yet the feedback mechanisms sustaining these traps remain poorly understood. This study addresses the research question what distinct feedback mechanisms perpetuate low productivity across four coexisting farming systems and system-specific leverage points. We used system dynamics modeling, collecting data from 375 households across three purposively selected districts (Dedo, Gera, and Sekoru), complemented by 21 key informant interviews and 8 focus group discussions. Causal loop diagrams (CLDs) were constructed to map feedback cycles within each system. Our results identified four major farming systems with observable disparities across each system. Farm income ranges from 15,691 Ethiopian Birr (ETB) (subsistence, 33% of households) to 46,114 ETB (coffee-honey, 33%). Meanwhile, frequent extension contact varies from 5.7% (subsistence) to 36.1% (wheat-livestock, 19%). Maize-livestock farmers (15%) overgraze at 2.3 TLU/ha (vs. recommended 0.8), mining soil fertility. Coffee-honey farmers face deforestation declined (canopy cover 60% --> 25%) and 81% climate impact perception; wheat-livestock farmers have only 8.3% formal credit access. CLDs identified distinct reinforcing traps: the food-labor nexus in subsistence, the soil mining trap in maize-livestock, the input-labor-pest complex in wheat-livestock, and the deforestation-pollination-price triad in coffee-honey. The findings indicate that each farming system is locked into a distinct self-reinforcing cycle, and that system-specific, testable intervention ranging from nutrition-sensitive safety nets for subsistence to climate-smart agroforestry for coffee-honey are required to break these cycles. These findings provide a dynamic, multi-system framework to guide policy design and future empirical evidence for sustainable smallholder agriculture in Ethiopia and similar contexts. CI - Copyright: (c) 2026 Chalchisa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Chalchisa, Tamiru AU - Chalchisa T AUID- ORCID: 0000-0003-4506-3307 AD - Department of Rural Development and Agricultural Extension, College of Agriculture and Veterinary Medicine, Jimma University, Jimma, Ethiopia. FAU - Amare, Abayineh AU - Amare A AD - Department of Rural Development and Agricultural Extension, College of Agriculture and Veterinary Medicine, Jimma University, Jimma, Ethiopia. FAU - Tolera, Teferi AU - Tolera T AD - Department of Rural Development and Agricultural Extension, College of Agriculture and Veterinary Medicine, Jimma University, Jimma, Ethiopia. FAU - Daniel, Meskelu AU - Daniel M AD - Department of Rural Development and Agricultural Extension, College of Agriculture and Veterinary Medicine, Jimma University, Jimma, Ethiopia. FAU - Gemeda, Dessalegn Obsi AU - Gemeda DO AD - Department of Natural Resource Management, College of Agriculture and Veterinary Medicine, Jimma University, Jimma, Ethiopia. AD - New Era and Development in Civil Engineering Research Group, Scientific Research Center, Al-Ayen Iraqi University (AUIQ), Nasiriyah, Thi-Qar, Iraq. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Ethiopia MH - *Agriculture/methods MH - *Conservation of Natural Resources MH - Humans PMC - PMC13716233 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 13:44 PHST- 2026/06/22 00:00 [received] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:44 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - PONE-D-26-29694 [pii] AID - 10.1371/journal.pone.0359541 [doi] PST - epublish SO - PLoS One. 2026 Oct 28;21(10):e0359541. doi: 10.1371/journal.pone.0359541. eCollection 2026. PMID- 42949985 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1751-3766 (Electronic) IS - 1751-3758 (Linking) VI - 20 IP - 1 DP - 2026 Dec 31 TI - Global stability analysis of within-host Zika virus-chikungunya virus co-infection with CTL immune responses. PG - 2749208 LID - 10.1080/17513758.2026.2749208 [doi] AB - We formulate and analyse a mathematical model for the within-host co-infection dynamics of Zika virus (ZIKV) and chikungunya virus (CHIKV), incorporating latently infected cells and cytotoxic T lymphocyte (CTL) immune responses. We establish the nonnegativity and boundedness of solutions. Using the next-generation matrix method, we derive the basic reproduction numbers (RZL) and (RCL) for the ZIKV and CHIKV submodels, respectively, and the overall reproduction number (R0L = maxRZL, RCL). Invasion reproduction numbers, (RZL,inv) and (RCL,inv), are derived to characterise the ability of either virus to invade an established infection. Global stability of the equilibria is established using Lyapunov functions. We investigate three therapeutic protocols: antiviral treatment blocking viral entry, treatment reducing viral production, and IL-2 therapy enhancing CTL responses. Sensitivity analysis of (RZL) and (RCL) is performed, and numerical simulations support the stability results. Simulations indicate that combined antiviral and immune-based interventions suppress co-infection by reducing viral replication and enhancing immune-mediated clearance. FAU - Elaiw, Ahmed M AU - Elaiw AM AUID- ORCID: 0000-0001-5030-633X AD - Department of Mathematics, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia. FAU - Alkhudhari, Zainab Y AU - Alkhudhari ZY AD - Department of Mathematics, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia. FAU - Almohaimeed, Ebtehal A AU - Almohaimeed EA AD - Department of Mathematics, College of Science, Qassim University, Buraydah, Saudi Arabia. FAU - Hobiny, Aatef D AU - Hobiny AD AD - Department of Mathematics, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - J Biol Dyn JT - Journal of biological dynamics JID - 101299725 SB - IM MH - *T-Lymphocytes, Cytotoxic/immunology MH - Humans MH - *Chikungunya virus/physiology/immunology MH - *Zika Virus/physiology/immunology MH - *Coinfection/immunology/virology MH - *Zika Virus Infection/immunology/virology/drug therapy MH - *Chikungunya Fever/immunology/virology/drug therapy MH - Models, Biological MH - Computer Simulation MH - Basic Reproduction Number OTO - NOTNLM OT - 34D20 OT - 92B05 OT - 92C37 OT - CTL immunity OT - Zika OT - chikungunya OT - global stability OT - sensitivity analysis EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 CRDT- 2026/10/28 13:44 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:44 [entrez] AID - 10.1080/17513758.2026.2749208 [doi] PST - ppublish SO - J Biol Dyn. 2026 Dec 31;20(1):2749208. doi: 10.1080/17513758.2026.2749208. Epub 2026 Oct 28. PMID- 42949970 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1091-6490 (Electronic) IS - 0027-8424 (Linking) VI - 123 IP - 44 DP - 2026 Nov 3 TI - Scaling laws link conserved gene-abundance distributions to adaptive expression in diatom communities worldwide. PG - e2615743123 LID - 10.1073/pnas.2615743123 [doi] AB - Marine microbes sustain Earth's major biogeochemical cycles, yet how they maintain stable ecosystem functions across diverse environments remains unclear. We analyzed metagenomic and metatranscriptomic data from diatom communities sampled during the Tara Oceans global expedition. While gene abundance distributions remain statistically similar across sample, gene expression varies systematically across geographical locations, a global pattern we show reflects predictable scaling laws. Global distributions, scaling relationships, and variability among sampling stations are captured by a stochastic model linking gene abundance dynamics to gene expression. While colder waters are associated with higher transcriptional activity and warmer regions show more selective gene usage, we find that transcript abundance increases nonlinearly with transcript richness. This general scaling is captured by our model and suggests that diatom communities balance high expression of core genes with specific functional diversity. These scaling relationships hold consistently across ocean basins, with temperature, used as an effective large-scale environmental descriptor whose signature cannot be fully disentangled from spatial structure, sufficing to predict transcriptional patterns from genetic composition. Our findings reveal how the stability of gene-abundance structure in diatom ecosystems coexists with transcriptional variability, enabling flexible functional responses. FAU - Pigani, Emanuele AU - Pigani E AUID- ORCID: 0000-0002-0566-0759 AD - Dipartimento di Fisica e Astronomia "Galileo Galilei", Universita di Padova, Padova 35131, Italy. ROR: https://ror.org/00240q980 AD - Stazione Zoologica Anton Dohrn, Napoli 80135, Italy. ROR: https://ror.org/03v5jj203 AD - Quantitative Life Sciences, The Abdus Salam International Centre for Theoretical Physics, Trieste 34151, Italy. ROR: https://ror.org/009gyvm78 FAU - Simoens, Kobe AU - Simoens K AD - Dipartimento di Fisica e Astronomia "Galileo Galilei", Universita di Padova, Padova 35131, Italy. ROR: https://ror.org/00240q980 FAU - Bernardi, Davide AU - Bernardi D AUID- ORCID: 0000-0002-7043-6606 AD - Dipartimento di Fisica e Astronomia "Galileo Galilei", Universita di Padova, Padova 35131, Italy. ROR: https://ror.org/00240q980 AD - National Biodiversity Future Center, Palermo 90133, Italy. FAU - Campese, Lucia AU - Campese L AUID- ORCID: 0000-0002-3543-738X AD - Stazione Zoologica Anton Dohrn, Napoli 80135, Italy. ROR: https://ror.org/03v5jj203 FAU - Iudicone, Daniele AU - Iudicone D AD - Stazione Zoologica Anton Dohrn, Napoli 80135, Italy. ROR: https://ror.org/03v5jj203 FAU - Azaele, Sandro AU - Azaele S AUID- ORCID: 0000-0002-5153-4833 AD - Dipartimento di Fisica e Astronomia "Galileo Galilei", Universita di Padova, Padova 35131, Italy. ROR: https://ror.org/00240q980 AD - National Biodiversity Future Center, Palermo 90133, Italy. FAU - Suweis, Samir AU - Suweis S AUID- ORCID: 0000-0002-1603-8375 AD - Dipartimento di Fisica e Astronomia "Galileo Galilei", Universita di Padova, Padova 35131, Italy. ROR: https://ror.org/00240q980 LA - eng GR - 862923/AtlantECO/ GR - 101059915/Biocean5D/ GR - C53D23003420001/NRRP/ GR - C93C22002810006/NRRP/ GR - 101082021/MARCO-BOLO/ PT - Journal Article DEP - 20261028 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 SB - IM MH - *Diatoms/genetics MH - Ecosystem MH - Oceans and Seas MH - Transcriptome MH - Metagenomics OTO - NOTNLM OT - functional stability OT - marine diatoms OT - metagenomics and metatranscriptomics OT - neutral models OT - stochastic gene expression COIS- Competing interests statement:The authors declare no competing interest. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 CRDT- 2026/10/28 13:44 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:44 [entrez] AID - 10.1073/pnas.2615743123 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2026 Nov 3;123(44):e2615743123. doi: 10.1073/pnas.2615743123. Epub 2026 Oct 28. PMID- 42949962 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1091-6490 (Electronic) IS - 0027-8424 (Linking) VI - 123 IP - 44 DP - 2026 Nov 3 TI - A strain-insensitive multiplexed soft microneedle patch for robust interstitial fluid biomarker monitoring. PG - e2611528123 LID - 10.1073/pnas.2611528123 [doi] AB - Wearable microneedle sensors analyze biomarkers in situ from interstitial fluids (ISF), enabling minimally invasive, precise, and continuous monitoring of metabolic diseases. However, conventional designs either lack adequate conformability to skin or suffer from electrode fractures during skin penetration and body motion, leading to signal instability that undermines reliable multiparameter acquisition. Here, we present a fully integrated system with a soft microneedle sensor array featuring nano-hill topographies on strain-resilient electrodes to delocalize strain via geometric confinement. This design reduces signal variability by 14-fold compared to conventional designs, enabling stable, simultaneous in situ monitoring of multiple ISF biomarkers. It achieves clinical-grade accuracy for continuous glucose monitoring (mean absolute relative difference of 10.8%) and captures dynamic synchronized variations in glucose, local tissue pH, and temperature in a living subject in real time. The system integrates a reusable electronic module with a disposable microneedle array with a sensor footprint of just 1.8 cm(2). By combining high signal stability with multiparameter synchrony in a compact form factor, this system offers a practical solution for reliable, real-time ISF analysis under dynamic in vivo conditions. FAU - Qiao, Zheng AU - Qiao Z AD - Department of Biomedical Engineering, National University of Singapore, Singapore 117583, Singapore. ROR: https://ror.org/01tgyzw49 FAU - Chen, Shuwen AU - Chen S AD - Institute of Medical Equipment Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China. ROR: https://ror.org/00p991c53 FAU - Liu, Shuoyan AU - Liu S AD - Department of Materials Science and Engineering, National University of Singapore, Singapore 117575, Singapore. ROR: https://ror.org/01tgyzw49 FAU - Madden, Leigh Edward AU - Madden LE AUID- ORCID: 0000-0003-1111-9288 AD - Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 308232, Singapore. ROR: https://ror.org/02e7b5302 FAU - Fan, Shicheng AU - Fan S AD - Department of Biomedical Engineering, National University of Singapore, Singapore 117583, Singapore. ROR: https://ror.org/01tgyzw49 FAU - Liang, Lanfeng AU - Liang L AUID- ORCID: 0000-0003-2127-904X AD - Department of Biomedical Engineering, National University of Singapore, Singapore 117583, Singapore. ROR: https://ror.org/01tgyzw49 FAU - Nai, Mui Hoon AU - Nai MH AUID- ORCID: 0000-0003-2823-1508 AD - Department of Biomedical Engineering, National University of Singapore, Singapore 117583, Singapore. ROR: https://ror.org/01tgyzw49 FAU - Lin, Baobao AU - Lin B AD - Department of Biomedical Engineering, National University of Singapore, Singapore 117583, Singapore. ROR: https://ror.org/01tgyzw49 FAU - Wu, Zixiong AU - Wu Z AD - Department of Biomedical Engineering, National University of Singapore, Singapore 117583, Singapore. ROR: https://ror.org/01tgyzw49 FAU - Li, Zhijie AU - Li Z AD - Department of Biomedical Engineering, National University of Singapore, Singapore 117583, Singapore. ROR: https://ror.org/01tgyzw49 FAU - Becker, David Lawrence AU - Becker DL AD - Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore 308232, Singapore. ROR: https://ror.org/02e7b5302 FAU - Wu, Changsheng AU - Wu C AUID- ORCID: 0000-0002-0921-6356 AD - Department of Materials Science and Engineering, National University of Singapore, Singapore 117575, Singapore. ROR: https://ror.org/01tgyzw49 AD - Institute for Health Innovation and Technology, (iHealthtech), National University of Singapore, Singapore 119276, Singapore. ROR: https://ror.org/01tgyzw49 FAU - Lim, Chwee Teck AU - Lim CT AUID- ORCID: 0000-0003-4019-9782 AD - Department of Biomedical Engineering, National University of Singapore, Singapore 117583, Singapore. ROR: https://ror.org/01tgyzw49 AD - Institute for Health Innovation and Technology, (iHealthtech), National University of Singapore, Singapore 119276, Singapore. ROR: https://ror.org/01tgyzw49 AD - Advanced Research and Technology Innovation Centre, National University of Singapore, Singapore 119276, Singapore. ROR: https://ror.org/01tgyzw49 AD - Mechanobiology Institute, National University of Singapore, Singapore 117411, Singapore. ROR: https://ror.org/01tgyzw49 LA - eng GR - A-0001415-06-00/NUS | Institute for Health Innovation and Technology, National University of Singapore (iHealthtech)/ GR - A-0009363-03-00/National University of Singapore (NUS)/ GR - A-005947-22-00/Advanced Research and Technology Innovation Centre, College of Design and Engineering, National University of Singapore (ARTIC)/ GR - E-468-00-0012-01/National University of Singapore (NUS)/ GR - 62501247/MOST | National Natural Science Foundation of China (NSFC)/ GR - 2024JCYJ069/Huazhong University of Science and Technology (HUST)/ GR - ZYYKFJJ2024001/Jinhua Institute of Intelligent Manufacturing/ PT - Journal Article DEP - 20261028 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 RN - 0 (Biomarkers) RN - IY9XDZ35W2 (Glucose) SB - IM MH - *Extracellular Fluid/chemistry/metabolism MH - *Biomarkers/analysis MH - *Needles MH - Humans MH - Glucose/analysis MH - Continuous Glucose Monitoring MH - *Biosensing Techniques/instrumentation/methods MH - Hydrogen-Ion Concentration MH - Equipment Design MH - Electrodes MH - Microneedle Drug Delivery MH - Wearable Electronic Devices OTO - NOTNLM OT - electrochemical sensors OT - flexible sensors OT - glucose monitoring OT - microneedle OT - wearable electronics COIS- Competing interests statement:The authors declare no competing interest. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 CRDT- 2026/10/28 13:43 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:43 [entrez] AID - 10.1073/pnas.2611528123 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2026 Nov 3;123(44):e2611528123. doi: 10.1073/pnas.2611528123. Epub 2026 Oct 28. PMID- 42949949 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1553-7404 (Electronic) IS - 1553-7390 (Print) IS - 1553-7390 (Linking) VI - 22 IP - 10 DP - 2026 Oct TI - Recombination rate and efficiency of linked selection in small and large stickleback populations. PG - e1012335 LID - 10.1371/journal.pgen.1012335 [doi] LID - e1012335 AB - Population genetic theory predicts that natural selection will be more efficient in large than small populations because genetic drift reduces the efficiency of selection in small populations. Small populations adapting to new environments can also be expected to evolve higher recombination rates to facilitate adaptation as well as to dissociate and purge harmful mutations. We tested these hypotheses (1) by investigating differences in the strength of association between nucleotide diversity (pi) and recombination rate across the genomes of nine-spined sticklebacks (Pungitius pungitius) from four small freshwater (mean Ne approximately 7 096) and four large marine (mean Ne = 376 828) populations, as well as (2) by comparing recombination rates between small and large populations using population specific linkage maps. We found the predicted positive correlation of pi with recombination rate from all but the smallest freshwater populations, suggesting prevalent linked selection even after accounting for variation in GC/CpG content, and gene density. Mean recombination rates did not differ between freshwater and marine populations, except that the smallest Ne freshwater population exhibited significantly elevated recombination rate. Exploratory GWAS analyses suggested a possible polygenic basis for recombination rate. These results suggest an important role for linked selection in reducing pi in low recombination regions especially in large populations. Moreover, as predicted by theory, at least one of the small freshwater populations appears to have evolved a higher recombination rate than its marine ancestors. CI - Copyright: (c) 2026 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Wang, Hongbo AU - Wang H AUID- ORCID: 0000-0001-8262-2580 AD - Area of Ecology and Biodiversity, School of Biological Sciences, The University of Hong Kong, Hong Kong SAR, China. FAU - Zhang, Chaowei AU - Zhang C AUID- ORCID: 0000-0003-4641-1756 AD - Area of Ecology and Biodiversity, School of Biological Sciences, The University of Hong Kong, Hong Kong SAR, China. FAU - Reid, Kerry AU - Reid K AD - Area of Ecology and Biodiversity, School of Biological Sciences, The University of Hong Kong, Hong Kong SAR, China. FAU - Merila, Juha AU - Merila J AUID- ORCID: 0000-0001-9614-0072 AD - Area of Ecology and Biodiversity, School of Biological Sciences, The University of Hong Kong, Hong Kong SAR, China. AD - Ecological Genetics Research Unit, Research Program in Organismal and Evolutionary Biology, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - PLoS Genet JT - PLoS genetics JID - 101239074 SB - IM MH - Animals MH - *Smegmamorpha/genetics MH - *Selection, Genetic/genetics MH - *Recombination, Genetic/genetics MH - Genetics, Population MH - Genetic Variation MH - Genetic Drift MH - Fresh Water MH - Genetic Linkage MH - Genome-Wide Association Study PMC - PMC13716167 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 13:43 PHST- 2026/04/01 00:00 [received] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:43 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - PGENETICS-D-26-00316 [pii] AID - 10.1371/journal.pgen.1012335 [doi] PST - epublish SO - PLoS Genet. 2026 Oct 28;22(10):e1012335. doi: 10.1371/journal.pgen.1012335. eCollection 2026 Oct. PMID- 42949940 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 0363-6771 (Print) IS - 0363-6771 (Linking) VI - 74 IP - 6 DP - 2026 Nov-Dec TI - Coronectomy as an alternative to extraction for mandibular third molars with high risk of paresthesia: a case report. PG - 58-61 AB - Coronectomy, a surgical alternative to dental extraction, involves the removal of the crown and retention of the intraosseous roots at least 3 mm below the alveolar crest. This report presents the clinical case of a 55-year-old woman who sought care due to pain and food accumulation in the region of the mandibular left third molar. Clinical evaluation and imaging tests revealed that the impacted tooth was close to the mandibular canal, and coronectomy was selected to avoid possible injury to the inferior alveolar nerve and consequent short- or long-term paresthesia. After administration of anesthesia and elevation of a full-thickness envelope flap, osteotomy was performed, and the crown was sectioned from the root buccolingually at the cementoenamel junction in such a way as to preserve the alveolar bone crest. The crown was carefully removed using elevators, and then the remaining root and surrounding bone were recontoured to maintain the root at least 3 mm below the margin of the alveolar crest without causing injury to the inferior alveolar nerve. Clinical and radiographic evaluations 2 years after the procedure revealed excellent tissue healing and good bone repair without root migration. The favorable outcome in this patient supports coronectomy as a viable and conservative treatment alternative in specific cases selected based on clear indications and careful planning, supported by adequate diagnostic imaging. It is important to schedule periodic follow-up evaluations to observe the behavior of the remaining roots. FAU - Mello, Luis Fernando AU - Mello LF FAU - Gonzalez, Alejandra Hortencia M AU - Gonzalez AHM FAU - Gomez, Ana Luiza AU - Gomez AL FAU - Texeira, Itamar Francisco AU - Texeira IF FAU - Andrade, Jose Rubens AU - Andrade JR FAU - Volpato, Luiz Evaristo Ricci AU - Volpato LER LA - eng PT - Case Reports PT - Journal Article PL - United States TA - Gen Dent JT - General dentistry JID - 7610466 SB - IM MH - Humans MH - Female MH - *Molar, Third/surgery/diagnostic imaging MH - Middle Aged MH - *Paresthesia/prevention & control/etiology MH - *Tooth Crown/surgery MH - Tooth Extraction/methods MH - Mandible/surgery MH - *Tooth, Impacted/surgery/diagnostic imaging MH - Tooth Root/surgery OTO - NOTNLM OT - coronectomy OT - mandibular canal OT - mandibular nerve OT - paresthesia OT - third molar OT - tooth extraction COIS- None reported. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 CRDT- 2026/10/28 13:43 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:43 [entrez] PST - ppublish SO - Gen Dent. 2026 Nov-Dec;74(6):58-61. PMID- 42949935 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 0363-6771 (Print) IS - 0363-6771 (Linking) VI - 74 IP - 6 DP - 2026 Nov-Dec TI - Laser surgical management of proliferative verrucous leukoplakia: a case report. PG - 29-33 AB - Proliferative verrucous leukoplakia (PVL) is an oral potentially malignant disorder that mainly affects middle-aged and older women. Its etiology remains unknown, and the disorder is not associated with known risk factors such as tobacco use. PVL lesions are characterized by a progressive, persistent, and recurrent nature, a fact that renders treatment challenging. This case report describes the treatment of PVL using a high-power laser surgical approach. A 54-year-old male smoker reported a chief concern of persistent white plaques in the mouth that had been present for approximately 5 years but had no associated symptoms. Intraoral clinical examination revealed white plaques of varying thicknesses whose surfaces were flat in some areas and granular in others; the lesions had irregular margins and involved the buccal mucosa bilaterally, extending to the retromolar trigone, oropharynx, hard palate, gingiva, and alveolar ridge. Incisional biopsies were performed in the bilateral buccal mucosa and oropharynx, and histopathologic analysis of the tissue revealed hyperkeratosis associated with mild epithelial dysplasia. The combination of clinical data and histopathologic findings led to the diagnosis of PVL. The patient was monitored, and additional biopsies were required over time. Due to the extent of the lesions and their nonhomogenous appearance, the patient was referred for evaluation and surgical management. A series of 9 surgical interventions with a high-power (980-nm) diode laser, operated in continuous mode at a power of 1.5 W, were performed at intervals of 45 to 60 days. Regular monitoring continues at 3-month intervals, and, 18 months after the first treatment, the patient has remained satisfied with the results, shows no significant recurrences, and has successfully reduced his tobacco use. Early diagnosis and continuous follow-up of PVL are essential to minimize the risk of malignancy and to ensure selection of the most appropriate therapeutic option. The results in this case add to the evidence that high-power laser therapy is an effective alternative for controlling these lesions. FAU - Silva, Thais Savier Pereira AU - Silva TSP FAU - Monteiro, Joao Pedro Denardi AU - Monteiro JPD FAU - Sugaya, Norberto Nobuo AU - Sugaya NN FAU - Azevedo, Luciane Hiramatzu AU - Azevedo LH FAU - Anbinder, Ana Lia AU - Anbinder AL FAU - Alves, Monica Ghislaine Oliveira AU - Alves MGO FAU - Almeida, Janete Dias AU - Almeida JD LA - eng PT - Case Reports PT - Journal Article PL - United States TA - Gen Dent JT - General dentistry JID - 7610466 SB - IM MH - Humans MH - Middle Aged MH - Male MH - *Leukoplakia, Oral/surgery/pathology MH - *Laser Therapy/methods OTO - NOTNLM OT - biopsy OT - laser therapy OT - leukoplakia OT - oral potentially malignant disorders OT - oropharynx COIS- None reported. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 CRDT- 2026/10/28 13:43 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:43 [entrez] PST - ppublish SO - Gen Dent. 2026 Nov-Dec;74(6):29-33. PMID- 42949934 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 0363-6771 (Print) IS - 0363-6771 (Linking) VI - 74 IP - 6 DP - 2026 Nov-Dec TI - Invasive cervical resorption: clinical management of necrotic pulp. PG - 22-27 AB - Invasive cervical resorption (ICR) is a pathologic and aggressive form of external root resorption that affects a small percentage of the population. Its etiology remains unclear and is considered multifactorial, involving trauma, orthodontic treatment, and internal bleaching, among other factors. This case report describes the clinical management of a patient diagnosed with class 3Ap ICR in the maxillary left canine. Cone beam computed tomography was crucial for diagnosis and treatment planning. The treatment included endodontic therapy, surgical intervention, and restoration with bioactive resin containing surface-treated prereacted glass ionomer filler. Follow-up after 6 months showed clinical and radiographic treatment success, with no signs of recurrence. This report highlights the importance of early diagnosis and interdisciplinary management of ICR to preserve affected teeth and avoid extraction. FAU - Villaca, Cibele Angelis Coelho AU - Villaca CAC FAU - Gracio, Ana Clara Maria Malta AU - Gracio ACMM FAU - Santos, Carlos Henrique Sales Dias AU - Santos CHSD FAU - Ferreira, Camila Lopes AU - Ferreira CL LA - eng PT - Case Reports PT - Journal Article PL - United States TA - Gen Dent JT - General dentistry JID - 7610466 SB - IM MH - Humans MH - *Root Resorption/diagnostic imaging/therapy/surgery MH - Cone-Beam Computed Tomography MH - *Dental Pulp Necrosis/therapy/diagnostic imaging MH - *Tooth Cervix/pathology/diagnostic imaging MH - Root Canal Therapy/methods MH - Cuspid/pathology/diagnostic imaging OTO - NOTNLM OT - S-PRG OT - bioactive resin OT - cone beam computed tomography OT - endodontics OT - invasive cervical resorption COIS- None reported. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 CRDT- 2026/10/28 13:43 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:43 [entrez] PST - ppublish SO - Gen Dent. 2026 Nov-Dec;74(6):22-27. PMID- 42949926 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - The diminishing returns of distance: Computational analysis of approach-avoidance behavior under threat of predation. PG - e0360036 LID - 10.1371/journal.pone.0360036 [doi] LID - e0360036 AB - Foraging under threat of predation is a paradigmatic scenario involving the interplay between approach and avoidance motivation across species. Previous foraging paradigms in humans have revealed aspects of approach-avoidance behavior and its neural underpinnings, but have generally involved simple choices such as a binary decision to stay or flee. Here, we present results from 40 adult participants who completed the Weighing Opportunity and Loss in Foraging (WOLF) task, a novel two-dimensional foraging context with threat of predation in which participants choose from five possible actions at each turn. We fit computational models in which choices depend on the subjective utility of each option, which itself depends on approach motivation (resource richness) and avoidance motivation (predator distance). Model comparison revealed that an inverse power law model, in which the marginal subjective utility of additional distance from the wolf diminished with wolf distance, outperformed a linear model. Model parameters explained more than 75% of the variance in task performance, mediated by the average resource richness of choices (for the approach-related parameter) and the number of deaths on the task (for the avoidance-related parameters). An exploratory analysis found that self-reported anxious arousal was associated with a more linear relationship between wolf distance and avoidance motivation. The results demonstrate that a behaviorally rich foraging paradigm in which participants select from multiple options at each turn can enable explicit modeling of subjective utilities in the interplay between approach and avoidance motivation. Future work can examine neural underpinnings of complex approach-avoidance behavior and their alteration in mental health disorders. CI - Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. FAU - Howlett, Jonathon R AU - Howlett JR AUID- ORCID: 0000-0002-6600-8609 AD - VA San Diego Healthcare System, San Diego, California, United States of America. AD - Department of Psychiatry, University of California San Diego, San Diego, California, United States of America. FAU - Simmons, Alan N AU - Simmons AN AD - VA San Diego Healthcare System, San Diego, California, United States of America. AD - Department of Psychiatry, University of California San Diego, San Diego, California, United States of America. FAU - Harle, Katia M AU - Harle KM AD - VA San Diego Healthcare System, San Diego, California, United States of America. AD - Department of Psychiatry, University of California San Diego, San Diego, California, United States of America. FAU - Stout, Daniel M AU - Stout DM AUID- ORCID: 0000-0001-8707-9206 AD - VA San Diego Healthcare System, San Diego, California, United States of America. AD - Department of Psychiatry, University of California San Diego, San Diego, California, United States of America. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Humans MH - *Predatory Behavior/physiology MH - Female MH - *Avoidance Learning/physiology MH - Male MH - Animals MH - Adult MH - *Choice Behavior/physiology MH - Motivation MH - Computer Simulation MH - Young Adult PMC - PMC13716165 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 13:43 PHST- 2025/04/14 00:00 [received] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:43 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - PONE-D-25-14922 [pii] AID - 10.1371/journal.pone.0360036 [doi] PST - epublish SO - PLoS One. 2026 Oct 28;21(10):e0360036. doi: 10.1371/journal.pone.0360036. eCollection 2026. PMID- 42949908 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - TFCdetector: Multivariable time series anomaly detection with time-frequency constraints. PG - e0361145 LID - 10.1371/journal.pone.0361145 [doi] LID - e0361145 AB - The multivariate time series collected from numerous sensors exhibit complex interwoven relationships, arising from both the coupling among different components and the temporal dependencies of fluctuations within each component. Disentangling these relationships remains a persistent challenge for the time series community. Existing reconstruction-based methods attempt to unravel the intertwined relationships through sequence decomposition and multi-scale modeling. However, the decomposed components remain mixtures of complex latent factors. Moreover, most methods overlook anomalies present in the training set, which inadvertently causes the model to develop a certain affinity toward anomalous patterns. To address these limitations, we propose TFCdetector. Motivated by the intrinsic properties of anomalies, TFCdetector leverages time-frequency domain analysis to explicitly suppress the model's representation learning of anomalies across diverse granularities and components. To further constrain the model's learning of anomalies, we propose an energy-correlation constraint loss that operates on both segment-level energy and sequence-level correlation. Notably, the adaptively updated memory storage block enhances the expression of normal patterns by leveraging the affinity between normal patterns in the input sequence and the memory storage block. Furthermore, the meticulously designed twin frequency attention enables the model to dynamically select local or global frequency information beneficial for the task. Extensive experiments on 1 synthetic dataset and 7 real-world datasets show that TFCdetector achieves competitive results in multivariate time series detection tasks.The implementation code is available at https://github.com/FanJY3/TFCdetector. CI - Copyright: (c) 2026 Fan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Fan, Jinyu AU - Fan J AD - College of Physics and Electronic Information Engineering, Qinghai Normal University, Xining, China. FAU - Zhao, Jianfei AU - Zhao J AD - College of Physics and Electronic Information Engineering, Qinghai Normal University, Xining, China. FAU - Ma, Jun AU - Ma J AUID- ORCID: 0009-0007-3495-8160 AD - College of Physics and Electronic Information Engineering, Qinghai Normal University, Xining, China. AD - College of Computer Technology and Application, Qinghai University, Xining, China. FAU - Cai, Yuxi AU - Cai Y AD - College of Computer Science and Technology, Xinjiang University, Urumqi, China. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Multivariate Analysis MH - Algorithms MH - Time Factors PMC - PMC13716231 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 13:36 PHST- 2026/03/13 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:36 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - PONE-D-26-12645 [pii] AID - 10.1371/journal.pone.0361145 [doi] PST - epublish SO - PLoS One. 2026 Oct 28;21(10):e0361145. doi: 10.1371/journal.pone.0361145. eCollection 2026. PMID- 42949902 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Patient safety culture in dental clinics: A cross-sectional study in Afghanistan. PG - e0359289 LID - 10.1371/journal.pone.0359289 [doi] LID - e0359289 AB - Healthcare safety is a major global concern since unsafe and low-quality services can lead to poor health outcomes and even harm to patients. Promoting a strong patient safety culture is a prerequisite for improving patient safety. The present study aimed to assess patient safety culture in dental clinics in Herat, Afghanistan in 2024. This cross-sectional study included 210 dental team members selected by random cluster sampling from 7 regions. The assessment tool was the Medical Office Survey on Patient Safety Culture (MOSOPS) modified for use in dentistry. A pilot study confirmed content validity and reliability (Cronbach's alpha > 0.70). Data were analyzed using descriptive statistics, bivariate tests (t-tests and ANOVA), and multiple linear regression to examine associations with patient safety culture. The total patient safety culture score was (0.70 +/- 0.10) (on a 0-1 scale), indicating a moderate level. The strongest mean scores were for organizational learning (0.78 +/- 0.14) and teamwork (0.77 +/- 0.17). The lowest score was observed for office processes and standardization (0.62 +/- 0.13). Multiple linear regression revealed that being female (B = 0.031, p = 0.049) and holding a managerial position (B = 0.073, p < 0.001) were significantly associated with higher patient safety culture scores. Overall, patient safety culture was generally favorable, although important organizational gaps remain. Future improvement efforts should focus on standardizing office processes and strengthening information exchange with external healthcare providers. In resource-limited settings such as Afghanistan, feasible interventions including locally adapted protocols, and ongoing managerial support and supervision may further strengthen patient safety culture. CI - Copyright: (c) 2026 Osmani et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Osmani, Safiyeh AU - Osmani S AD - Hariva institute of Higher Education, Herat, Afghanistan. AD - Student Research Committee, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran. FAU - Emrani, Zahra AU - Emrani Z AD - Social Determinants of Health Research Center, Basic Sciences Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran. AD - Department of Health Economics and Management Sciences, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran. FAU - Akhlaghi, Saeed AU - Akhlaghi S AD - Social Determinants of Health Research Center, Basic Sciences Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran. AD - Department of Biostatistics, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran. FAU - Shahraki, Amin AU - Shahraki A AUID- ORCID: 0009-0008-1229-1892 AD - Student Research Committee, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran. AD - Department of Health Economics and Management Sciences, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran. FAU - Moghri, Javad AU - Moghri J AD - Social Determinants of Health Research Center, Basic Sciences Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran. AD - Department of Health Economics and Management Sciences, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran. FAU - Kokabisaghi, Fatemeh AU - Kokabisaghi F AUID- ORCID: 0000-0002-9745-8784 AD - Social Determinants of Health Research Center, Basic Sciences Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran. AD - Department of Health Economics and Management Sciences, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Humans MH - Afghanistan MH - Cross-Sectional Studies MH - *Patient Safety/standards MH - *Dental Clinics/standards/organization & administration MH - Female MH - Male MH - Adult MH - Surveys and Questionnaires MH - Pilot Projects MH - Organizational Culture MH - *Safety Management PMC - PMC13716210 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 13:35 PHST- 2026/07/06 00:00 [received] PHST- 2026/09/13 00:00 [accepted] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:35 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - PONE-D-26-31020 [pii] AID - 10.1371/journal.pone.0359289 [doi] PST - epublish SO - PLoS One. 2026 Oct 28;21(10):e0359289. doi: 10.1371/journal.pone.0359289. eCollection 2026. PMID- 42949900 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Environmental drivers of taste characteristics in wild edible plants: Effects of topographic gradients and human harvesting evaluated using dwarf bamboo culms. PG - e0360962 LID - 10.1371/journal.pone.0360962 [doi] LID - e0360962 AB - Wild edible plants grow in natural environments with little direct human management; consequently, their productivity and quality frequently exhibit pronounced spatiotemporal variability, making stable supply difficult. This variability represents a major constraint on the exploitation of wild edible plants as local food resources, and overcoming it requires an ecological understanding of the factors affecting variation in both productivity and quality. Here, we examined the effects of human harvesting and environmental factors on the taste characteristics of young culms of Sasa kurilensis. No statistically significant differences were detected between harvested and control plots in any major taste index, including umami, bitter aftertaste, astringency, umami richness, and sweetness (measured as Brix values). In contrast, taste characteristics showed substantial variation among study plots, suggesting a strong association with local environmental conditions. Elevation and slope angle were significantly correlated with bitter aftertaste, umami, and sweetness; plots at higher elevations and on steeper slopes tended to produce plants with a less bitter aftertaste, and higher sweetness and umami. Principal component analysis integrating multiple taste indices revealed that the major axis of taste variation was significantly associated with elevation, and that harvesting treatment had no detectable effect. These results suggest that the taste characteristics of young culms in the study area are governed primarily by topographic gradients rather than by human harvesting. Although wild edible plants are important food resources in boreal and temperate forests worldwide, many are poorly managed. By quantitatively establishing relationships between taste characteristics and environmental factors in young culms, this study provides a scientific basis for determining the optimal timing, location, and selection criteria for wild edible plant harvesting, thereby contributing to the development of sustainable use strategies. CI - Copyright: (c) 2026 Katayama et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Katayama, Noboru AU - Katayama N AUID- ORCID: 0000-0003-1602-7497 AD - General Education, Faculty of Commerce, Otaru University of Commerce, Otaru, Hokkaido, Japan. FAU - Ryuda, Noriko AU - Ryuda N AD - Analytical Research Center for Experimental Sciences, Saga University, Saga, Japan. FAU - Ryuda, Masasuke AU - Ryuda M AD - Analytical Research Center for Experimental Sciences, Saga University, Saga, Japan. FAU - Takagi, Kentaro AU - Takagi K AUID- ORCID: 0000-0002-1321-2841 AD - Teshio Experimental Forest, Field Science Center for Northern Biosphere, Hokkaido University, Horonobe, Hokkaido, Japan. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Humans MH - *Taste MH - *Plants, Edible/growth & development MH - *Sasa/physiology/growth & development MH - *Environment PMC - PMC13716176 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 13:35 PHST- 2026/05/09 00:00 [received] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:35 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - PONE-D-26-22953 [pii] AID - 10.1371/journal.pone.0360962 [doi] PST - epublish SO - PLoS One. 2026 Oct 28;21(10):e0360962. doi: 10.1371/journal.pone.0360962. eCollection 2026. PMID- 42949897 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Geographic proximity to Down syndrome specialty clinics for individuals born with Down syndrome, 1999-2020 in Texas. PG - e0361583 LID - 10.1371/journal.pone.0361583 [doi] LID - e0361583 AB - BACKGROUND: While effective national clinic networks exist for a select few conditions, the availability and use of multidisciplinary specialty care for most genetic or congenital conditions, including Down syndrome (DS), remain uneven. This uneven distribution creates significant geographic and social gradients in healthcare access. To understand these differences within a geographically expansive and socio-demographically diverse setting, this study quantified travel burden to pediatric DS specialty clinics in Texas, identifying differences based on key maternal socio-demographic and geographic factors. METHODS: A cross-sectional analysis of 8,201 individuals with DS presumed living and 477 deceased individuals with DS born between 1999-2020 from the Texas Birth Defects Registry was performed. One-way driving time and distance along the road networks from the maternal residence to the nearest seven pediatric DS specialty clinics were calculated using ArcGIS Pro. Travel burden was analyzed by maternal race/ethnicity, education, age and residence at delivery. Travel burden measures were categorized as low, moderate to high, and very high. RESULTS: Among 8,678 live‑born individuals with DS, 45% resided over 90 miles from the closest in-state DS specialty clinic, and 41% faced one‑way driving times exceeding two hours. Those born to mothers in border counties experienced the greatest travel burden, with median one-way driving times exceeding 7 hours. Rural residents had a four times greater travel burden than urban residents. Individuals born to Hispanic mothers traveled twice as far as those born to non-Hispanic mothers. Residents of high‑poverty tracts had a median one-way distance of approximately 150 miles, triple the 35 miles seen in low‑poverty tracts. CONCLUSION: Our findings suggest that substantial travel burden to DS specialty clinics exists in Texas, affecting individuals with DS born to Hispanic and younger mothers, as well as those in rural, border, and concentrated-poverty regions. Further research is needed to explore life course residential history and clinic utilization to address these differences. CI - Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. FAU - Gandhi, Henal A AU - Gandhi HA AUID- ORCID: 0000-0002-1156-515X AD - Birth Defects Epidemiology and Surveillance Branch, Texas Department of State Health Services, Austin, Texas, United States of America. FAU - Betancourt, Dayana AU - Betancourt D AD - Birth Defects Epidemiology and Surveillance Branch, Texas Department of State Health Services, Austin, Texas, United States of America. FAU - Shumate, Charles AU - Shumate C AD - Birth Defects Epidemiology and Surveillance Branch, Texas Department of State Health Services, Austin, Texas, United States of America. FAU - Allred, Rachel AU - Allred R AD - Birth Defects Epidemiology and Surveillance Branch, Texas Department of State Health Services, Austin, Texas, United States of America. FAU - Dixon, Ashley AU - Dixon A AD - Birth Defects Epidemiology and Surveillance Branch, Texas Department of State Health Services, Austin, Texas, United States of America. FAU - Kilburn, Madeline AU - Kilburn M AD - Birth Defects Epidemiology and Surveillance Branch, Texas Department of State Health Services, Austin, Texas, United States of America. FAU - Singletary, Claire AU - Singletary C AD - Department of Pediatrics and Department of Obstetrics, Gynecology, and Reproductive Sciences, McGovern Medical School at UTHealth Houston, Genetic Counseling Program, The University of Texas MD Anderson Cancer Center UTHealth Houston Graduate School of Biomedical Sciences, University of Texas Health Science Center at Houston, Houston, Texas, United States of America. FAU - Riconda, Daniel AU - Riconda D AD - Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States of America. FAU - Agopian, A J AU - Agopian AJ AD - Department of Epidemiology, University of Texas Health Science Center at Houston (UTHealth) School of Public Health, Houston, Texas, United States of America. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Humans MH - Texas/epidemiology MH - Female MH - *Down Syndrome/epidemiology/therapy MH - Cross-Sectional Studies MH - *Health Services Accessibility/statistics & numerical data MH - Male MH - Travel/statistics & numerical data MH - Adult MH - Child MH - Registries MH - Adolescent MH - Child, Preschool MH - Geography PMC - PMC13716173 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 13:35 PHST- 2025/12/11 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:35 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - PONE-D-25-64488 [pii] AID - 10.1371/journal.pone.0361583 [doi] PST - epublish SO - PLoS One. 2026 Oct 28;21(10):e0361583. doi: 10.1371/journal.pone.0361583. eCollection 2026. PMID- 42949893 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Biological and environmental correlates of cougar (Puma concolor) survival in west central Alberta. PG - e0357879 LID - 10.1371/journal.pone.0357879 [doi] LID - e0357879 AB - Managing large carnivores benefits from understanding their vital rates, including survival. For cougars (Puma concolor), the hazards relevant to survival across the landscape are poorly understood. The risks that landscape features present can change temporally, increasing survival in one season while creating mortality risk in another. Between 2016 and 2021, 80 cougars were radio-collared and tracked in west central Alberta, Canada, for a total of 1082 cougar months with a mean monitoring time of 13.5 months/individual. We documented 37 deaths, determined causes of mortality, generated survival estimates using Kaplan-Meier models by sex and age class annually and for two seasons (i.e., warm and cold), and investigated factors affecting survival using Cox proportional hazard models. Humans were responsible for 29 of the 37 documented mortalities, including harvest, removal of problem individuals, landowner removals, incidental trapping, and vehicle collisions, with harvest representing the largest single source of mortality (16/37 deaths) for both sexes. However, we were unable to detect a difference in survival estimates between sexes or age classes and found no evidence of a male-biased harvest. Mean annual survival was 0.60 (95% CI: 0.50-0.72) and varied seasonally with lower survival in the cold season (0.62, 95% CI: 0.52-0.74) than the warm season (0.96, 95% CI: 0.92-1.00). Using Cox proportional hazards models (Andersen-Gill), model selection identified a best-supported mixed model combining biological, environmental, and anthropogenic features: season, proximity to residences, proximity to secondary roads, linear-feature crossing rate, and step length. CI - Copyright: (c) 2026 Littlefair et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Littlefair, Carter J AU - Littlefair CJ AUID- ORCID: 0009-0009-8646-1564 AD - Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada. FAU - Frame, Paul F AU - Frame PF AD - Fish and Wildlife Stewardship, Alberta Environment and Protected Areas, Edmonton, Alberta, Canada. FAU - Edwards, Mark A AU - Edwards MA AD - Office of the Chief Scientist, Alberta Environment and Protected Areas, Edmonton, Alberta, Canada. AD - Department of Renewable Resources, University of Alberta, Edmonton, Alberta, Canada. FAU - Derocher, Andrew E AU - Derocher AE AD - Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Animals MH - Alberta MH - Male MH - Seasons MH - Female MH - *Puma/physiology MH - Proportional Hazards Models MH - Environment MH - Conservation of Natural Resources PMC - PMC13716201 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 13:35 PHST- 2026/03/25 00:00 [received] PHST- 2026/08/24 00:00 [accepted] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:35 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - PONE-D-26-14840 [pii] AID - 10.1371/journal.pone.0357879 [doi] PST - epublish SO - PLoS One. 2026 Oct 28;21(10):e0357879. doi: 10.1371/journal.pone.0357879. eCollection 2026. PMID- 42949880 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Causal decomposition of Black-white disparities in NIH's selection of proposals for panel discussion. PG - e0358838 LID - 10.1371/journal.pone.0358838 [doi] LID - e0358838 AB - Prior research on NIH R01 grants peer review found that selection of proposals for panel discussion constituted the decision point with the largest contribution to Black-white NIH funding disparities. We use a causal decomposition framework to understand what factors causally contribute to reducing Black-white disparities in selection into discussion, and to assess how recent procedural changes may affect Black-white disparities in selection into discussion. We first use causal decomposition methodology to explore whether hypothetical interventions eliminating Black-white disparities and/or differences in attributes would reduce Black-white disparities in selection of proposals for panel discussion. The attributes we consider include those associated with applicants (degree, career stage, their institution's funding bin) and their submitted proposals (application type, amended status, Preliminary Overall Impact Score, and NIH assigned Administering Organization and Integrated Review Group). Under reasonable assumptions, we find that among the attributes considered, Preliminary Overall Impact Score was the only one that, after equalizing, could dissolve Black-white disparities in selection into discussion. In addition, we conduct a thought experiment to explore potential impacts of the NIH's move to binary scoring for the Investigator and Environment criteria in the new Simplified Peer Review Framework on Black-white disparities in selection into discussion. Analyses from our thought experiment suggest that changing to binary scoring of the Investigator-and-Environment factor may not have any effect on disparities in selection into discussion. Overall, under the assumptions of the causal decomposition framework, these results suggest that efforts aimed at reducing disparities in selection into discussion should focus on identifying upstream, policy-relevant, and practically feasible interventions for eliminating racial disparities in Preliminary Overall Impact Scores. In contrast, the transition to the Simplified Peer Review Framework is unlikely to substantially reduce disparities in selection into discussion. CI - Copyright: (c) 2026 Prakash et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Prakash, Shreya AU - Prakash S AUID- ORCID: 0000-0003-2406-188X AD - Department of Statistics, University of Washington, Seattle, Washington, United States of America. FAU - Lee, Carole J AU - Lee CJ AUID- ORCID: 0000-0002-5323-9205 AD - Department of Philosophy, University of Washington, Seattle, Washington, United States of America. AD - Center for an Informed Public, University of Washington, Seattle, Washington, United States of America. FAU - Erosheva, Elena A AU - Erosheva EA AD - Department of Statistics, University of Washington, Seattle, Washington, United States of America. AD - School of Social Work, University of Washington, Seattle, Washington, United States of America. AD - Center for Statistics and the Social Sciences, University of Washington, Seattle, Washington, United States of America. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - United States MH - Humans MH - *White People MH - *National Institutes of Health (U.S.) MH - *Black or African American MH - White PMC - PMC13716228 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 13:33 PHST- 2025/09/02 00:00 [received] PHST- 2026/09/03 00:00 [accepted] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:33 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - PONE-D-25-47858 [pii] AID - 10.1371/journal.pone.0358838 [doi] PST - epublish SO - PLoS One. 2026 Oct 28;21(10):e0358838. doi: 10.1371/journal.pone.0358838. eCollection 2026. PMID- 42949879 OWN - NLM STAT- Publisher LR - 20261028 IS - 1944-7922 (Electronic) IS - 1060-3271 (Linking) DP - 2026 Oct 28 TI - Development of an HPLC-UV-Vis Method for the Determination of Epirubicin Residues on Stainless-Steel Surfaces. LID - qsag098 [pii] LID - 10.1093/jaoacint/qsag098 [doi] AB - BACKGROUND: Cross-contamination control is essential in pharmaceutical manufacturing for highly potent and cytotoxic active pharmaceutical ingredients used in oncology products. Epirubicin, an anthracycline antineoplastic agent, requires reliable residue monitoring after cleaning because residual carryover on shared manufacturing equipment may pose quality and safety risks. OBJECTIVE: This study aimed to develop a practical HPLC-UV-Vis method for epirubicin determination, evaluate its chromatographic performance using solution-based standards and quality-control samples, and apply the method to swab samples collected from contaminated stainless-steel surfaces following different cleaning procedures. METHODS: Chromatographic separation was achieved on a Poroshell 120 SB-C8 column using an isocratic mobile phase of acetonitrile and purified water containing 0.1% orthophosphoric acid (28:72, v/v) at 0.5 mL/min, with UV-Vis detection at 480 nm, within the visible region. Chromatographic performance was evaluated using solution-based standards and quality-control samples for selectivity, carryover, linearity, sensitivity, accuracy, precision, stability, and system suitability. AISI 304 matte stainless-steel plates were contaminated with epirubicin solution, subjected to different cleaning procedures, and sampled by swabbing. Surface recovery was assessed separately at a single spiking level. RESULTS: The method was linear over 20-1000 ng/mL. The LOQ was established at 20 ng/mL (S/N = 10.2). The single-level surface-recovery assessment yielded a recovery of 70.5%. Cleaning procedures including 2% sodium hydroxide resulted in no quantifiable parent epirubicin peak under the applied chromatographic conditions. CONCLUSION: The developed HPLC-UV-Vis method showed suitable chromatographic performance for epirubicin determination under the investigated solution-based conditions and was applied to residue monitoring in swab samples collected from stainless-steel surfaces following cleaning. Because surface recovery was assessed at only one spiking level, the complete surface-sampling and extraction procedure was not comprehensively validated. HIGHLIGHTS: The developed HPLC-UV-Vis method was applied to epirubicin residue monitoring in swab samples collected from stainless-steel surfaces, with no quantifiable parent epirubicin peak observed after sodium hydroxide-containing cleaning procedures. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of AOAC INTERNATIONAL. FAU - Inan, Gozde Gulin AU - Inan GG AUID- ORCID: 0000-0002-1997-410X AD - Department of Analytical Chemistry, Faculty of Pharmacy, University of Health Sciences, Istanbul, Turkiye. AD - Department of Analytical Chemistry, Faculty of Pharmacy; Institute of Health Science, Istanbul University, Istanbul, Turkey. FAU - Danis, Ibrahim AU - Danis I AUID- ORCID: 0000-0003-4646-4129 AD - Drug Research and Application Center; Istanbul University, Istanbul, Turkiye. FAU - Unal, Durisehvar Ozer AU - Unal DO AUID- ORCID: 0000-0003-0754-1240 AD - Drug Research and Application Center; Istanbul University, Istanbul, Turkiye. AD - Department of Analytical Chemistry, Faculty of Pharmacy, Istanbul University, Istanbul, Turkiye. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - J AOAC Int JT - Journal of AOAC International JID - 9215446 SB - IM EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 13:33 PHST- 2026/06/20 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:33 [entrez] AID - 9003929 [pii] AID - 10.1093/jaoacint/qsag098 [doi] PST - aheadofprint SO - J AOAC Int. 2026 Oct 28:qsag098. doi: 10.1093/jaoacint/qsag098. PMID- 42949877 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261028 IS - 2767-3375 (Electronic) IS - 2767-3375 (Linking) VI - 6 IP - 10 DP - 2026 TI - Vitamin C deficiency in a Northern Saskatchewan Population, Canada. PG - e0007464 LID - 10.1371/journal.pgph.0007464 [doi] LID - e0007464 AB - Vitamin C deficiency, shaped by systemic inequities and disrupted food systems, remains a concern in remote Indigenous communities. In Northern Saskatchewan, clinicians observed increasing suspected scurvy among adults from geographically isolated First Nations communities. We conducted an exploratory retrospective review of adults who underwent clinician-initiated serum ascorbic acid testing at the La Ronge Medical Clinic from January 2011 to March 2024. Testing was based on clinical suspicion or perceived risk rather than population-based screening. Vitamin C deficiency was defined as <11 micromol/L and hypovitaminosis C as 11-23 micromol/L. Demographic, clinical, and testing-indication data were extracted, with exploratory comparisons by First Nations status, sex, age, and documented characteristic scurvy features. Study governance followed OCAP principles through partnerships with Lac La Ronge Indian Band Health Services and the Northern Inter-Tribal Health Authority. Among 48 individuals with analyzable results, 27 (56%) had vitamin C deficiency and 10 (21%) had hypovitaminosis C. Deficiency was more common among First Nations than non-First Nations patients (66% versus 20%, p = 0.014). Dermatologic and oral manifestations were common indications for testing, although deficiency was also identified among patients without documented characteristic scurvy features. The high proportion of deficiency among tested patients suggests that vitamin C deficiency is an important clinical concern in this setting. However, this clinically selected sample should not be interpreted as estimating population-level prevalence. Given restricted laboratory access and the technical challenges of serum ascorbic acid testing, empiric supplementation may be a practical option for high-risk individuals. Longer-term responses should prioritize Indigenous-led food sovereignty, culturally grounded interventions, and policies addressing food access and underlying structural inequities. CI - Copyright: (c) 2026 Irvine et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Irvine, Jeffrey David Connor AU - Irvine JDC AUID- ORCID: 0009-0000-4391-9904 AD - Northern Medical Services, Department of Family Medicine, College of Medicine, University of Saskatchewan, Saskatchewan, Canada. FAU - Atreyu, Yoseph C AU - Atreyu YC AD - Northern Medical Services, Department of Family Medicine, College of Medicine, University of Saskatchewan, Saskatchewan, Canada. FAU - Carlson, Mary AU - Carlson M AD - Lac La Ronge Indian Band Health Services, La Ronge, Saskatchewan, Canada. FAU - Quinn, Brian AU - Quinn B AUID- ORCID: 0009-0006-8456-6352 AD - Population Health Unit, Saskatchewan Health Authority, Saskatchewan, Canada. FAU - Irvine, James AU - Irvine J AUID- ORCID: 0000-0001-5104-903X AD - Department of Family Medicine, College of Medicine, University of Saskatchewan, Saskatchewan, Canada. FAU - Ndubuka, Nnamdi AU - Ndubuka N AUID- ORCID: 0000-0002-1200-5787 AD - Northern Inter-Tribal Health Authority, Prince Albert, Saskatchewan, Canada. AD - Community Health and Epidemiology, College of Medicine, University of Saskatchewan, Saskatchewan, Canada. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - PLOS Glob Public Health JT - PLOS global public health JID - 9918283779606676 PMC - PMC13716197 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 13:33 PHST- 2025/11/28 00:00 [received] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:33 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - PGPH-D-25-03694 [pii] AID - 10.1371/journal.pgph.0007464 [doi] PST - epublish SO - PLOS Glob Public Health. 2026 Oct 28;6(10):e0007464. doi: 10.1371/journal.pgph.0007464. eCollection 2026. PMID- 42949874 OWN - NLM STAT- Publisher LR - 20261028 IS - 1525-3163 (Electronic) IS - 0021-8812 (Linking) DP - 2026 Oct 28 TI - Genome-wide association study of eye muscle area and intramuscular fat in pure- and crossbred beef cattle. LID - skag340 [pii] LID - 10.1093/jas/skag340 [doi] AB - The genetic improvement of meat quality traits, such as eye muscle area (EMA) and intramuscular fat (IMF), is a primary goal for increasing the profitability and efficiency of the beef industry. However, it remains unclear how the genetic architecture of these traits differs across diverse breed types, which complicates the development of accurate multi-breed genomic evaluations. The objective of this study was to identify common genomic regions associated with ultrasound EMA and IMF across five beef cattle populations, including two purebreds (Angus and Hereford) and three crossbreds (Brangus, Santa Gertrudis, and Speckle Park). Genome-wide association studies (GWAS) were performed using a linear mixed model. Phenotypic and genomic data from 66,313 bulls and 56,200 heifers for EMA, and 65,230 bulls and 54,905 heifers for IMF, were analysed using medium-density SNP arrays. The results showed some associated genomic regions that were shared across breeds, likely due to their similar genetic origins or breeding objectives, for example: chromosome 2 at approximately 0.57-14 Mb for both bull and heifer EMA and IMF in Santa Gertrudis and Speckle Park; chromosome 5 at positions approximately 45-50 Mb for bull and heifer IMF in Brangus and Santa Gertrudis and for approximately 105-106 for bull EMA in Angus and Hereford breeds; chromosome 7 at approximately 90-91 Mb for bull EMA and heifer IMF in Angus and Hereford, chromosome 17 at approximately 60-62 Mb for bull IMF in Angus and Hereford; and chromosome 20 at approximately 4-5 Mb for heifer IMF in Angus and Hereford. Some genomic signals were breed-specific, likely reflecting differences in allele frequencies or linkage disequilibrium between pure- and crossbred populations. This research demonstrates that while some genetic drivers of meat quality are conserved across breeds, breed-specific markers are essential for accurate genomic prediction. These findings provide a biological basis for applying marker weighting in genomic evaluations to enhance selection accuracy for meat quality traits in multi-breed evaluations. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the American Society of Animal Science. FAU - Manzari, Zeinab AU - Manzari Z AD - AGBU, a Joint Venture of the NSW Department of Primary Industries and Regional Development and the University of New England, Armidale, New South Wales, 2351, Australia. FAU - Connors, Natalie K AU - Connors NK AD - AGBU, a Joint Venture of the NSW Department of Primary Industries and Regional Development and the University of New England, Armidale, New South Wales, 2351, Australia. FAU - van der Werf, Julius H J AU - van der Werf JHJ AD - School of Environmental and Rural Science, University of New England, Armidale, New South Wales, 2351, Australia. FAU - Johnston, David J AU - Johnston DJ AD - AGBU, a Joint Venture of the NSW Department of Primary Industries and Regional Development and the University of New England, Armidale, New South Wales, 2351, Australia. FAU - Ferdosi, Mohammad H AU - Ferdosi MH AD - AGBU, a Joint Venture of the NSW Department of Primary Industries and Regional Development and the University of New England, Armidale, New South Wales, 2351, Australia. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Anim Sci JT - Journal of animal science JID - 8003002 SB - IM OAB - Identifying the genetic factors that influence meat quality is important for profitable beef production. This research looked at several beef cattle breeds, including cross- and purebreds, to find specific parts of the DNA (genomic regions) linked to muscle area and meat fat. Our results revealed some genomic regions are common across breeds with similar breeding goals, while others are unique to the specific cross- or purebreds. These findings help us better understand which markers to prioritising and weighting in genomic models to improve the accuracy of animal selection and the efficiency of meat production. OABL- eng OTO - NOTNLM OT - Beef cattle OT - Genome-wide Association Study OT - Genomic regions OT - Meat traits EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 13:33 PHST- 2025/12/07 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:33 [entrez] AID - 9003919 [pii] AID - 10.1093/jas/skag340 [doi] PST - aheadofprint SO - J Anim Sci. 2026 Oct 28:skag340. doi: 10.1093/jas/skag340. PMID- 42949863 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1941-5923 (Electronic) IS - 1941-5923 (Linking) VI - 27 DP - 2026 Oct 28 TI - Bilateral Iatrogenic Pneumothorax After Acupuncture in a 26-Year-Old Woman Presenting With Cough, Pleuritic Chest Pain, and Tachycardia: A Case Report. PG - e954143 LID - 10.12659/AJCR.954143 [doi] AB - BACKGROUND Acupuncture is widely used and generally safe, but pneumothorax is a serious complication of needling in the neck, shoulder, or upper back. Bilateral pneumothorax is rare and can present with nonspecific symptoms, delaying diagnosis. This case report highlights the presentation of immediate cough during upper-back needling, delayed presentation with bilateral pneumothorax despite preserved oxygenation, and radiographic regression during specialist-supervised conservative inpatient management. CASE REPORT A 26-year-old woman presented to the emergency department with persistent cough, pleuritic chest pain, and difficulty taking a full breath 2 days after acupuncture in the trapezius region. She was otherwise hemodynamically stable, with a blood pressure of 132/93 mm Hg and oxygen saturation of 100% on room air, but had marked tachycardia (140 beats/min at triage) and decreased right-sided breath sounds. Chest radiography showed bilateral apical pneumothoraces measuring 21 mm on the right and 17 mm on the left. Computed tomography pulmonary angiography excluded pulmonary embolism and confirmed bilateral pneumothoraces up to 23 mm without hemothorax. Preserved blood pressure and oxygenation, absence of respiratory compromise, and availability of specialist inpatient monitoring supported conservative management without pleural intervention. Serial radiographs showed regression to 6 mm on the right and 9 mm on the left before discharge. CONCLUSIONS Recent acupuncture involving the neck, shoulder, or upper back should prompt consideration of pneumothorax in patients with chest pain, cough, dyspnea, or unexplained tachycardia, even when oxygen saturation is normal. Conservative inpatient management may be feasible only in carefully selected patients with bilateral procedure-related pneumothorax under close specialist clinical and radiographic surveillance. FAU - Nozewski, Jakub AU - Nozewski J AUID- ORCID: 0000-0002-8765-233X AD - Emergency Medicine Unit, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Bydgoszcz, Poland. AD - Emergency Department, University Hospital No. 2 (Dr. Jan Biziel Memorial Hospital), Bydgoszcz, Poland. FAU - Zalewska, Bernadeta AU - Zalewska B AD - Emergency Department, University Hospital No. 2 (Dr. Jan Biziel Memorial Hospital), Bydgoszcz, Poland. FAU - Zwierko, Beata AU - Zwierko B AUID- ORCID: 0009-0008-9640-7636 AD - Department of Clinical Neuropsychology, Faculty of Health Sciences, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Bydgoszcz, Poland. LA - eng PT - Case Reports PT - Journal Article DEP - 20261028 PL - United States TA - Am J Case Rep JT - The American journal of case reports JID - 101489566 SB - IM MH - Humans MH - Female MH - *Pneumothorax/etiology/diagnostic imaging MH - Adult MH - Chest Pain/etiology MH - Cough/etiology MH - Tachycardia/etiology MH - *Acupuncture Therapy/adverse effects MH - Iatrogenic Disease EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 CRDT- 2026/10/28 13:23 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:23 [entrez] AID - 954143 [pii] AID - 10.12659/AJCR.954143 [doi] PST - epublish SO - Am J Case Rep. 2026 Oct 28;27:e954143. doi: 10.12659/AJCR.954143. PMID- 42949853 OWN - NLM STAT- Publisher LR - 20261028 IS - 1527-3792 (Electronic) IS - 0022-5347 (Linking) DP - 2026 Oct 28 TI - The Missing Comparator: Smoking Cessation Cost-Effectiveness in the Era of Novel Bladder Cancer Therapies. PG - 101097JU0000000000005359 LID - 10.1097/JU.0000000000005359 [doi] FAU - Stroud, Margaret AU - Stroud M AD - Department of Urology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. FAU - Talwar, Ruchika AU - Talwar R AD - Department of Urology, Vanderbilt Health, Nashville, Tennessee. AD - Office of Population Health, Vanderbilt Health, Nashville, Tennessee. FAU - Bjurlin, Marc A AU - Bjurlin MA AUID- ORCID: 0000-0003-3500-8849 AD - Department of Urology, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. AD - Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA. LA - eng PT - Editorial DEP - 20261028 PL - United States TA - J Urol JT - The Journal of urology JID - 0376374 SB - IM OTO - NOTNLM OT - bladder cancer OT - cost-effectiveness OT - non-muscle-invasive bladder cancer OT - quality-adjusted life-years OT - smoking cessation EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 13:13 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:13 [entrez] AID - 10.1097/JU.0000000000005359 [doi] PST - aheadofprint SO - J Urol. 2026 Oct 28:101097JU0000000000005359. doi: 10.1097/JU.0000000000005359. PMID- 42949852 OWN - NLM STAT- Publisher LR - 20261028 IS - 2040-3372 (Electronic) IS - 2040-3364 (Linking) DP - 2026 Oct 28 TI - Interfacial hydrogen bonding and quantum confinement synergistically control triplet-energy-transfer-mediated photocatalysis by perovskite nanocrystals. LID - 10.1039/d6nr03307e [doi] AB - Triplet-energy-transfer (TET) photocatalysis by semiconductor nanocrystals (NCs) relies on short-range Dexter transfer across the NC-substrate interface, making catalytic efficiency highly sensitive to both NC photophysics and substrate organization. While lead halide perovskite NCs have recently emerged as potent triplet sensitizers, current strategies largely rely on carboxylate-mediated substrate anchoring to facilitate interfacial TET. Generalizable substrate-organization strategies and their interplay with quantum confinement in governing photocatalytic reactivity remain unresolved. Here, we demonstrate that CsPbBr(3) NCs catalyze TET-mediated [2 + 2] photocycloaddition of chalcone derivatives and reveal that interfacial hydrogen bonding and quantum confinement synergistically govern photocatalytic reactivity. Native ammonium ligands of the NCs promote substrate recognition through hydrogen-bonding interactions, organizing chalcone substrates at the NC surface without covalent anchoring. Stern-Volmer analysis and transient absorption spectroscopy reveal pronounced size-dependent TET dynamics, with quantum-confined NCs exhibiting substantially enhanced triplet sensitization. These effects translate directly into photocatalytic performance, enabling efficient intermolecular photocycloadditions of diverse chalcone derivatives and selective heterocoupling with alkene partners through preferential chalcone sensitization. Control experiments further demonstrate that efficient photocatalysis requires both interfacial hydrogen bonding and quantum confinement. This work establishes an interfacial design principle for perovskite-NC triplet photocatalysis and provides new insight into controlling energy-transfer reactions at semiconductor nanocrystal interfaces. FAU - Zahoor, Sadia AU - Zahoor S AUID- ORCID: 0009-0000-5640-6294 AD - Department of Chemistry, Emory University, Atlanta, Georgia, 30322, USA. feng.zhai@emory.edu. FAU - Soni, Ashish AU - Soni A AUID- ORCID: 0000-0001-7429-576X AD - Department of Chemistry, Emory University, Atlanta, Georgia, 30322, USA. feng.zhai@emory.edu. FAU - Zhao, Joel AU - Zhao J AUID- ORCID: 0009-0003-7997-1992 AD - Department of Chemistry, Emory University, Atlanta, Georgia, 30322, USA. feng.zhai@emory.edu. FAU - Singh, Jyoti AU - Singh J AUID- ORCID: 0000-0003-4693-3992 AD - Department of Chemistry, Emory University, Atlanta, Georgia, 30322, USA. feng.zhai@emory.edu. FAU - Ho, Eric A AU - Ho EA AUID- ORCID: 0000-0002-0689-7651 AD - Department of Chemistry, Emory University, Atlanta, Georgia, 30322, USA. feng.zhai@emory.edu. FAU - Zhai, Feng AU - Zhai F AUID- ORCID: 0000-0001-7892-3188 AD - Department of Chemistry, Emory University, Atlanta, Georgia, 30322, USA. feng.zhai@emory.edu. FAU - Wang, Lili AU - Wang L AUID- ORCID: 0000-0002-6298-0302 AD - Department of Chemistry, Emory University, Atlanta, Georgia, 30322, USA. feng.zhai@emory.edu. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Nanoscale JT - Nanoscale JID - 101525249 SB - IM EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 13:13 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:13 [entrez] AID - 10.1039/d6nr03307e [doi] PST - aheadofprint SO - Nanoscale. 2026 Oct 28. doi: 10.1039/d6nr03307e. PMID- 42949849 OWN - NLM STAT- Publisher LR - 20261028 IS - 1527-3792 (Electronic) IS - 0022-5347 (Linking) DP - 2026 Oct 28 TI - A Randomized Trial of Multimodal Prehabilitation Before Robotic-Assisted Radical Prostatectomy to Improve Postoperative Quality of Life and Functional Outcomes. PG - 101097JU0000000000005365 LID - 10.1097/JU.0000000000005365 [doi] AB - PURPOSE: Robotic-assisted radical prostatectomy (RARP) causes postoperative impairments. Although prehabilitation has shown benefits in surgical populations, data specific to RARP remain limited. We evaluated whether multimodal prehabilitation improves early postoperative health-related quality of life (HRQoL), functional recovery and mental health. MATERIALS AND METHODS: Prospective, single-center randomized trial including men with prostate cancer scheduled for RARP (May 2022- September 2024). Participants were randomized to a 4-week multimodal prehabilitation program including physical and pelvic floor muscle training, nutritional counselling and psychological support or to usual care. The primary outcome was change in the EORTC QLQ-C30 global health status score from baseline to 1 month after surgery. Secondary endpoints included functional and perioperative outcomes at 1, 3 and 6 months. RESULTS: Ninety-five of 100 randomized patients were analyzed (53 prehabilitation, 42 control). At 1-month post-operatively, global health status score was higher in the prehabilitation group (adjusted mean difference +15 points, 0-100 scale; 95% confidence interval (CI) 7-22; p<0.001). Improvements at 1-month were also observed in several HRQoL domains, mental health and urinary continence (mean difference in ICIQ-UI SF -2.7; p=0.032). No differences were detected in physical performance or erectile function. Prehabilitation benefits attenuated over time, with the global health status difference decreasing to +4.5 points at 6 months (-1.9 points/month; p = 0.024), whereas improvements in selected HRQoL domains, mental health, and ICIQ-UI SF were maintained throughout follow-up. CONCLUSIONS: Multimodal prehabilitation before RARP is feasible and should be integrated into clinical practice, as it enhances postoperative recovery and patient-centered outcomes. FAU - Carbonell, Enric AU - Carbonell E AD - Department of Urology, Hospital Clinic de Barcelona, Barcelona, Spain. AD - Universitat de Barcelona, Barcelona, Spain. FAU - Dana, Fernando AU - Dana F AD - Universitat de Barcelona, Barcelona, Spain. AD - Department of Anesthesiology, Hospital Clinic de Barcelona, Barcelona, Spain. FAU - Tena, Beatriz AU - Tena B AD - Universitat de Barcelona, Barcelona, Spain. AD - Department of Anesthesiology, Hospital Clinic de Barcelona, Barcelona, Spain. AD - Fundacio de Recerca Clinic Barcelona - IDIBAPS, Barcelona, Spain. FAU - Sebio-Garcia, Raquel AU - Sebio-Garcia R AD - Fundacio de Recerca Clinic Barcelona - IDIBAPS, Barcelona, Spain. AD - Department of Physical Medicine and Rehabilitation, Hospital Clinic de Barcelona, Barcelona, Spain. FAU - Salinas-Gonzalez, Raquel AU - Salinas-Gonzalez R AD - Department of Physical Medicine and Rehabilitation, Hospital Clinic de Barcelona, Barcelona, Spain. FAU - Romano-Andrioni, Barbara AU - Romano-Andrioni B AD - Department of Endocrinology and Nutrition, Hospital Clinic de Barcelona, Barcelona, Spain. FAU - Capitan, David AU - Capitan D AD - Department of Anesthesiology, Hospital Clinic de Barcelona, Barcelona, Spain. FAU - Teres, Silvia AU - Teres S AD - Institute of Digestive and Metabolic disorders, Hospital Clinic de Barcelona, Barcelona, Spain. FAU - Mercader, Claudia AU - Mercader C AD - Department of Urology, Hospital Clinic de Barcelona, Barcelona, Spain. FAU - Vendrell, Marina AU - Vendrell M AD - Universitat de Barcelona, Barcelona, Spain. AD - Department of Anesthesiology, Hospital Clinic de Barcelona, Barcelona, Spain. FAU - Musquera, Mireia AU - Musquera M AD - Department of Urology, Hospital Clinic de Barcelona, Barcelona, Spain. AD - Universitat de Barcelona, Barcelona, Spain. FAU - Laxe, Sara AU - Laxe S AD - Fundacio de Recerca Clinic Barcelona - IDIBAPS, Barcelona, Spain. AD - Department of Physical Medicine and Rehabilitation, Hospital Clinic de Barcelona, Barcelona, Spain. FAU - Ribal, Maria J AU - Ribal MJ AD - Department of Urology, Hospital Clinic de Barcelona, Barcelona, Spain. AD - Universitat de Barcelona, Barcelona, Spain. FAU - Vickers, Andrew J AU - Vickers AJ AD - Department of Epidemiology and Biostatistics, Memorial Sloan Kettering Cancer Center, New York, NY, USA. FAU - Alcaraz, Antonio AU - Alcaraz A AD - Department of Urology, Hospital Clinic de Barcelona, Barcelona, Spain. AD - Universitat de Barcelona, Barcelona, Spain. AD - Fundacio de Recerca Clinic Barcelona - IDIBAPS, Barcelona, Spain. FAU - Martinez-Palli, Graciela AU - Martinez-Palli G AD - Department of Anesthesiology, Hospital Clinic de Barcelona, Barcelona, Spain. AD - Fundacio de Recerca Clinic Barcelona - IDIBAPS, Barcelona, Spain. FAU - Vilaseca, Antoni AU - Vilaseca A AD - Department of Urology, Hospital Clinic de Barcelona, Barcelona, Spain. AD - Universitat de Barcelona, Barcelona, Spain. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Urol JT - The Journal of urology JID - 0376374 SB - IM OTO - NOTNLM OT - health-related quality of life OT - multimodal prehabilitation OT - prehabilitation OT - prostate cancer OT - radical prostatectomy OT - robotic-assisted radical prostatectomy EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 13:12 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:12 [entrez] AID - 10.1097/JU.0000000000005365 [doi] PST - aheadofprint SO - J Urol. 2026 Oct 28:101097JU0000000000005365. doi: 10.1097/JU.0000000000005365. PMID- 42949843 OWN - NLM STAT- Publisher LR - 20261028 IS - 2380-0194 (Electronic) IS - 2380-0186 (Linking) DP - 2026 Oct 28 TI - Posterior Cervical Foraminotomy: A Step-by-Step Surgical Technique Guide by the Cervical Spine Research Society. LID - 10.1097/BSD.0000000000002208 [doi] AB - INTRODUCTION: Posterior cervical foraminotomy is a motion-sparing technique utilized to treat radiculopathy resulting from herniated discs and/or osseous foraminal stenosis. This procedure allows for decompression of the target nerve root without the need for an anterior approach, thus mitigating the risk profile of anterior procedures. Posterior cervical foraminotomy has demonstrated equivalent outcomes to anterior cervical discectomy and fusion in improving pain and weakness, while allowing for rapid recovery. SURGICAL TECHNIQUE: Careful evaluation of preoperative imaging is necessary to assess the location and nature of the compressive pathology. The procedure consists of resecting the lateral lamina and medial aspect of the corresponding facet to decompress the exiting nerve root by unroofing the foramen. Discectomy can be performed as needed. Complications include nerve root injury, durotomy, incomplete symptomatic relief, and rarely instability and/or postoperative kyphosis. CONCLUSION: Posterior cervical foraminotomy is an effective, motion-sparing treatment for cervical radiculopathy that provides durable symptomatic relief and rapid recovery in well-selected patients while avoiding the risk profile associated with anterior cervical approaches. Careful attention to each surgical step and ensuring an adequate decompression are necessary for maximizing patient benefit and minimizing potential complications. CI - Copyright (c) 2026 Wolters Kluwer Health, LLC. All rights reserved. FAU - Albert, Andrew AU - Albert A AD - Texas Back Institute, Plano, TX. FAU - Satin, Alexander AU - Satin A AD - Texas Back Institute, Plano, TX. FAU - Derman, Peter AU - Derman P AD - Texas Back Institute, Plano, TX. FAU - Su, Brian W AU - Su BW AD - Marin Health Spine Institute, Marin, CA. FAU - Ghogawala, Zoher AU - Ghogawala Z AD - Lahey Hospital and Medical Center, Burlington, MA. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Clin Spine Surg JT - Clinical spine surgery JID - 101675083 SB - IM OTO - NOTNLM OT - cervical radiculopathy OT - indications OT - neuroforaminal stenosis OT - posterior cervical foraminotomy COIS- The authors declare no conflict of interest. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 13:03 PHST- 2025/11/24 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 13:03 [entrez] AID - 01933606-990000000-00865 [pii] AID - 10.1097/BSD.0000000000002208 [doi] PST - aheadofprint SO - Clin Spine Surg. 2026 Oct 28. doi: 10.1097/BSD.0000000000002208. PMID- 42949819 OWN - NLM STAT- Publisher LR - 20261028 IS - 2192-2659 (Electronic) IS - 2192-2640 (Linking) DP - 2026 Oct 28 TI - Bone Organoids Across the Development-to-Remodeling Continuum: From Tissue Formation to Functional Competence and Translation. LID - e71911 [pii] LID - 10.1002/adhm.71911 [doi] AB - Bone organoids are emerging as three-dimensional skeletal models that reproduce selected aspects of bone development, organization, and function under controlled conditions. Physiologically relevant models require more than cellular aggregation and mineral deposition; they must progressively acquire organized matrices, multicellular interactions, vascular and metabolic support, and mechanical responsiveness. In this review, we examine developmental principles guiding bone-organoid construction, including skeletal hierarchy, cellular niches, ossification pathways, and spatiotemporal signaling. We then discuss current strategies based on skeletal, mesenchymal stromal, and pluripotent stem cells, together with advances in biomaterials, three-dimensional bioprinting, perfusion, and microfluidic culture. To organize these developments, we introduce the development-to-remodeling continuum (DRC), which distinguishes tissue formation from multicellular integration, adaptive responsiveness, and remodeling competence. We assess collagen-mineral organization, osteocyte-network formation, vascular and metabolic maturation, osteoblast-osteoclast coupling, marrow and immune integration, and mechanical adaptation. We further evaluate applications in developmental and disease modeling, drug assessment, personalized modeling, and bone regeneration. Current limitations include variability, incomplete maturation, transport constraints, and insufficient functional benchmarking. By linking construction strategies to specific bone-related functions and the evidence supporting them, the DRC provides a framework for evaluating functional claims in relation to intended application and translational readiness. CI - (c) 2026 The Author(s). Advanced Healthcare Materials published by Wiley-VCH GmbH. FAU - Tang, Xijie AU - Tang X AD - Department of Orthopaedics, Wuhan Third Hospital, Wuhan, China. FAU - Xu, Haijia AU - Xu H AD - Department of Orthopaedics, Wuhan Third Hospital, Wuhan, China. FAU - Liu, Xiangzhong AU - Liu X AD - Department of Orthopaedics, Wuhan Third Hospital, Wuhan, China. FAU - Yang, Yi AU - Yang Y AD - Hubei Key Laboratory of Exercise Training and Monitoring, Department of Sports Medicine, Wuhan Sports University, Wuhan, China. FAU - Li, Zhanghua AU - Li Z AUID- ORCID: 0009-0003-7718-8742 AD - Department of Orthopaedics, Wuhan Third Hospital, Wuhan, China. LA - eng GR - 2025AFD655/Natural Science Foundation of Hubei Province/ GR - 14th Five-Year-Plan Advantageous and Characteristic Disciplines (Groups) of Colleges and Universities in Hubei Province/ GR - 2026020301040138/Wuhan Natural Science Foundation Clinical Research Key Project of Municipal Medical Institutions/ PT - Journal Article PT - Review DEP - 20261028 PL - Germany TA - Adv Healthc Mater JT - Advanced healthcare materials JID - 101581613 SB - IM OTO - NOTNLM OT - biomaterials OT - bone organoids OT - bone regeneration OT - bone-on-a-chip OT - functional maturation OT - skeletal development OT - three-dimensional bioprinting EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 12:33 PHST- 2026/10/10 00:00 [revised] PHST- 2026/08/24 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 12:33 [entrez] AID - e71911 [pii] AID - 10.1002/adhm.71911 [doi] PST - aheadofprint SO - Adv Healthc Mater. 2026 Oct 28:e71911. doi: 10.1002/adhm.71911. PMID- 42949817 OWN - NLM STAT- Publisher LR - 20261028 IS - 1477-9234 (Electronic) IS - 1477-9226 (Linking) DP - 2026 Oct 28 TI - Electrifying oxygen atom transfer reactions: utilizing pathways of OER and ORR for organic synthesis. LID - 10.1039/d6dt01709f [doi] AB - Oxygen atom transfer (OAT) reactions are key transformations in the synthesis of commodity and fine chemicals, with existing processes relying on stoichiometric oxidants and sometimes harsh reaction conditions. Electrification enables these reactions (e-OAT) to occur at room temperature and replaces chemical oxidants with low-cost electricity using sustainable O atom donors such as water or O(2) to reduce environmental impact. At the same time, e-OAT separates the thermodynamic driving force provided by electricity from the oxygen atom source, providing potential for a unique selectivity that cannot easily be achieved in traditional methods using chemical oxidants. In order to spark new developments in the field of e-OAT, this Perspective summarizes the fundamental mechanistic aspects and successful examples of chemical OAT to highlight the relation as well as the development potential of e-OAT reactions across epoxidation, C-H hydroxylation, sulfoxidation, N-oxidation and phosphine oxidation. Covering both homogeneous and heterogeneous catalysts, the state-of-the-art and most recent developments of these e-OAT reactions are included herein. We elaborate on the role of different reactive intermediate types and the design principles for catalysts and reaction conditions, outlining ways to achieve high reaction selectivity for e-OAT processes over parasitic side reactions such as O(2) evolution, as well as the strategies for facilitating challenging reactions such as partial methane oxidation to methanol. To inspire innovation, we visualize an overlap of the active intermediates of e-OAT with OER and ORR catalysis platforms, with an opportunity for repurposing the existing successful catalysts for e-OAT. Finally, we showcase with selected examples that the successful application of e-OAT catalysis will eventually require combined control over catalyst species as well as reactor design to accomplish demanding target reactions, such as epoxidation, methane hydroxylation or oxidative N(2) fixation, at optimal performance. FAU - Pletsch, Helena AU - Pletsch H AUID- ORCID: 0000-0001-7190-3811 AD - Technical University of Munich, TUM School of Natural Sciences, Department of Chemistry & Catalysis Research Center (CRC), Lichtenbergstr. 4, 85748 Garching, Germany. FAU - Kilaparthi, Sravan Kumar AU - Kilaparthi SK AUID- ORCID: 0000-0003-1717-8916 AD - Department of Chemical Engineering, Faculty of Chemical Sciences and Technologies, University of Castilla-La Mancha, Avda. Camilo Jose Cela 12, E-13071, Ciudad Real, Spain. AD - University of Antwerp, Faculty of Applied Engineering, Department of Biochemical and Chemical Engineering, Research Group Applied Electrochemistry & Catalysis (ELCAT), Universiteitsplein 1, 2610 Antwerp, Belgium. dominik.halter@uantwerp.be. FAU - Halter, Dominik P AU - Halter DP AUID- ORCID: 0000-0003-0733-8955 AD - Technical University of Munich, TUM School of Natural Sciences, Department of Chemistry & Catalysis Research Center (CRC), Lichtenbergstr. 4, 85748 Garching, Germany. AD - University of Antwerp, Faculty of Applied Engineering, Department of Biochemical and Chemical Engineering, Research Group Applied Electrochemistry & Catalysis (ELCAT), Universiteitsplein 1, 2610 Antwerp, Belgium. dominik.halter@uantwerp.be. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - Dalton Trans JT - Dalton transactions (Cambridge, England : 2003) JID - 101176026 SB - IM EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 12:33 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 12:33 [entrez] AID - 10.1039/d6dt01709f [doi] PST - aheadofprint SO - Dalton Trans. 2026 Oct 28. doi: 10.1039/d6dt01709f. PMID- 42949810 OWN - NLM STAT- Publisher LR - 20261028 IS - 2192-2659 (Electronic) IS - 2192-2640 (Linking) DP - 2026 Oct 28 TI - A Mixed‑Valence Cu/Mn Bimetallic Coordination Polymer Nano‑Assembly Co‑Loaded With Taurine and Lenvatinib for Synergistic Tumor Chemo-Metalloimmunotherapy. LID - e71908 [pii] LID - 10.1002/adhm.71908 [doi] AB - Despite considerable progress in cancer immunotherapy, the therapeutic efficacy of current cancer therapy continues to be hampered by the immunosuppressive tumor microenvironment (ITME), which is characterized by acidification, hypoxia, elevated H(2)O(2) levels, and aberrant angiogenesis. While transition metal-based nanomaterials hold great promise for modulating the ITME, their effectiveness is often constrained by insufficient reactive oxygen species (ROS) generation, low immunogenicity, poor immune cell infiltration, and T-cell exhaustion. To address these issues, we developed a bimetallic Cu/Mn-doped nanoplatform, CMPTL, which further integrates acid-dissociable taurine and the angiogenesis inhibitor lenvatinib for malignant solid tumor therapy. This nanoplatform simultaneously triggers violent ROS and cuproptosis, leading to mitochondrial damage and the release of mitochondrial DNA. The subsequent activation of the cGAS-STING pathway, synergistically enhanced by Mn(2+), initiates robust immune responses. Additionally, taurine helps alleviate T-cell exhaustion, while lenvatinib promotes tumor vessel normalization, leading to comprehensive remodeling of the TIME. Together, these multimodal mechanisms endow CMPTL with potent antitumor activity, positioning it as a promising candidate for cancer therapy. CI - (c) 2026 Wiley-VCH GmbH. FAU - Luo, Yangwen AU - Luo Y AD - Frontiers Science Center For Transformative Molecules, School of Chemistry and Chemical Engineering, Zhangjiang Institute for Asdvanced Study, Shanghai Jiao Tong University, Shanghai, P. R. China. AD - Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, and School of Pharmacy, Shanghai Jiao Tong University, Shanghai, P. R. China. AD - Department of Hepatic Oncology, Shanghai Geriatric Medical Center, Shanghai, P. R. China. FAU - Sun, Yunhan AU - Sun Y AD - Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, and School of Pharmacy, Shanghai Jiao Tong University, Shanghai, P. R. China. FAU - Zhang, Wenkai AU - Zhang W AUID- ORCID: 0000-0002-4087-4875 AD - Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, and School of Pharmacy, Shanghai Jiao Tong University, Shanghai, P. R. China. FAU - Shi, Xiaoying AU - Shi X AD - Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, and School of Pharmacy, Shanghai Jiao Tong University, Shanghai, P. R. China. FAU - Shen, Tianyi AU - Shen T AD - Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, and School of Pharmacy, Shanghai Jiao Tong University, Shanghai, P. R. China. FAU - Gu, Muge AU - Gu M AD - Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, and School of Pharmacy, Shanghai Jiao Tong University, Shanghai, P. R. China. FAU - Shen, Jiefeng AU - Shen J AUID- ORCID: 0000-0002-7551-7405 AD - College of Chemistry and Chemical Engineering, Donghua University, Shanghai, P. R. China. FAU - Yuan, Wei-En AU - Yuan WE AUID- ORCID: 0000-0003-0241-9449 AD - Engineering Research Center of Cell & Therapeutic Antibody, Ministry of Education, and School of Pharmacy, Shanghai Jiao Tong University, Shanghai, P. R. China. FAU - Yang, Guoqiang AU - Yang G AUID- ORCID: 0000-0002-8356-6653 AD - Frontiers Science Center For Transformative Molecules, School of Chemistry and Chemical Engineering, Zhangjiang Institute for Asdvanced Study, Shanghai Jiao Tong University, Shanghai, P. R. China. FAU - Yang, Hua AU - Yang H AUID- ORCID: 0000-0003-0346-2844 AD - Department of Hepatic Oncology, Shanghai Geriatric Medical Center, Shanghai, P. R. China. AD - Department of Liver Surgery and Transplantation, Key Laboratory of Carcinogenesis and Cancer Invasion of Ministry of Education, Liver Cancer Institute and Zhongshan Hospital, Fudan University, Shanghai, P. R. China. LA - eng GR - 2232023A-07/Fundamental Research Funds for the Central Universities/ GR - 25X010202131/Fundamental Research Funds for the Central Universities/ GR - 22071148/National Natural Science Foundation of China/ GR - KJXM-2022-02-01/Shanghai Jiao Tong University/ PT - Journal Article DEP - 20261028 PL - Germany TA - Adv Healthc Mater JT - Advanced healthcare materials JID - 101581613 SB - IM OTO - NOTNLM OT - cGAS-STING OT - cancer therapy OT - copper OT - taurine OT - tumor microenvironment EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 12:23 PHST- 2026/10/16 00:00 [revised] PHST- 2026/05/29 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 12:23 [entrez] AID - e71908 [pii] AID - 10.1002/adhm.71908 [doi] PST - aheadofprint SO - Adv Healthc Mater. 2026 Oct 28:e71908. doi: 10.1002/adhm.71908. PMID- 42949809 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2473-4209 (Electronic) IS - 0094-2405 (Linking) VI - 53 IP - 11 DP - 2026 Nov TI - Regularization in multiplexed PET image reconstruction. LID - e70738 [pii] LID - 10.1002/mp.70738 [doi] AB - BACKGROUND: Multiplexed PET (mPET) using one tracer labeled with a positron plus prompt gamma ray emitter is limited by low sensitivity to triple-photon coincidences (two annihilation photons and one prompt gamma photon), leading to high noise in the reconstructed images. PURPOSE: The use of regularization has been previously shown to increase the image quality of single tracer PET images under low signal-to-noise ratio conditions. We will demonstrate the use of regularization in simultaneously acquired multi-tracer mPET data reconstruction. METHODS: First, we present how a maximum a posteriori expectation maximization solution can be obtained for simultaneously acquired multi-isotope PET data. Next, we use a Monte Carlo simulation of an image quality phantom mPET acquisition with a pure positron ((18)F) and a positron plus prompt gamma ((44)Sc) emitter to evaluate the performance of different regularization methods. RESULTS: For a baseline regularization method to simultaneously reconstruct both activity distributions, we show that a relative difference penalty (RDP) provides the best tradeoff of the penalty functions tested in image quality and quantitative accuracy for a simulated hot sphere phantom compared to quadratic and log-cosh penalty functions, with a contrast-to-noise ratio (CNR) in (18)F/(44)Sc of over 60% higher on average for the RDP compared to the quadratic penalty in the largest spheres. However, this regularization approach has up to 2x worse reconstructed inter-tracer crosstalk ratio compared to unregularized reconstruction. Finally, we present a further optimization by alternating reconstruction of the two individual tracer images with regularization to achieve a CNR in the largest (18)F/(44)Sc spheres with an up to 35% improvement on average over the simultaneous RDP-based regularization reconstruction results described above, while maintaining a 10%-20% lower inter-tracer crosstalk ratio over the unregularized reconstruction. CONCLUSIONS: For the selected radionuclides, simulated system and phantom, we demonstrate proof-of-principle that using regularization applied to an alternating update form of EM achieves significantly higher CNR in both tracers compared to unregularized alternating update OSEM and regularized simultaneous OSEM, with significantly lower crosstalk in the prompt gamma emitter reconstruction than the regularized simultaneous OSEM. CI - (c) 2026 American Association of Physicists in Medicine. FAU - Foster, Jackson W AU - Foster JW AD - Department of Electrical Engineering, Stanford University, Stanford, California, USA. AD - Molecular Imaging Program, Department of Radiology, Stanford University, Stanford, California, USA. FAU - He, Wen AU - He W AD - Molecular Imaging Program, Department of Radiology, Stanford University, Stanford, California, USA. FAU - Chinn, Garry AU - Chinn G AD - Molecular Imaging Program, Department of Radiology, Stanford University, Stanford, California, USA. FAU - Levin, Craig S AU - Levin CS AD - Department of Electrical Engineering, Stanford University, Stanford, California, USA. AD - Molecular Imaging Program, Department of Radiology, Stanford University, Stanford, California, USA. AD - Department of Bioengineering, Stanford University, Stanford, California, USA. AD - Department of Physics, Stanford University, Stanford, California, USA. LA - eng GR - F31CA265205/CA/NCI NIH HHS/United States GR - Dr. Ralph and Marian Falk Medical Research Trust/ GR - Stanford Bio-X/ GR - Society of Nuclear Medicine and Molecular Imaging - Mars Shot/ PT - Journal Article PL - United States TA - Med Phys JT - Medical physics JID - 0425746 SB - IM MH - *Positron-Emission Tomography/methods MH - *Image Processing, Computer-Assisted/methods MH - Phantoms, Imaging MH - Monte Carlo Method MH - Signal-To-Noise Ratio OTO - NOTNLM OT - Maximum A Posteriori Expectation Maximization (MAP-EM) OT - Monte Carlo simulation OT - image reconstruction OT - joint estimation OT - list mode OT - multiplexed PET OT - positron emission tomography (PET) OT - positron plus prompt gamma emitter OT - regularization EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 CRDT- 2026/10/28 12:23 PHST- 2026/07/28 00:00 [revised] PHST- 2026/01/27 00:00 [received] PHST- 2026/07/31 00:00 [accepted] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 12:23 [entrez] AID - e70738 [pii] AID - 10.1002/mp.70738 [doi] PST - ppublish SO - Med Phys. 2026 Nov;53(11):e70738. doi: 10.1002/mp.70738. PMID- 42949807 OWN - NLM STAT- Publisher LR - 20261028 IS - 1533-4287 (Electronic) IS - 1064-8011 (Linking) DP - 2026 Oct 27 TI - The Relationship of Countermovement Jump Performance to Operational Readiness in United States Air Force Special Operators. LID - 10.1519/JSC.0000000000005704 [doi] AB - Tomlitz, ST, Dawes, J, Abel, MG, and Potenza, MA. The relationship of countermovement jump performance to operational readiness in United States air force special operators. J Strength Cond Res XX(X): 000-000, 2026-Operational readiness signifies the degree to which special operations soldiers are physically capable of accomplishing occupational tasks. The purpose of this study was to examine the relationship between selected countermovement jump (CMJ)-derived neuromuscular performance characteristics and operational readiness assessment scores among United States Air Force Pararescuemen (PJs). Thirty-six male PJs (age = 37.06 +/- 5.98 years, height = 178.73 +/- 6.27 cm, body mass (BM) = 86.51 +/- 8.97 kg, body mass index = 27.06 +/- 2.24 kg/m2) performed a series of tests, including a CMJ test and components of the tier II Operator Fitness Test (OFT). The CMJ-derived metrics (jump height, concentric impulse (CI), reactive strength modified, peak power (PP), PP/BM, concentric peak force, peak landing force, landing impulse, CI asymmetry, countermovement depth, eccentric PP/BM, force at zero velocity) were correlated with the tier II OFT events using bivariate Spearman's correlations. The significance level of this study was set at 0.05). Intragroup comparisons revealed that OHRQoL scores at follow-up times (T1, T3, T6, T12, T18) were significantly lower than baseline (T0) for all groups (P<0,01), except for the social disability and handicap domain which improved in some groups (p 0.05). CONCLUSION: Functional immediate loading of SIs or MIs with mandibular overdentures led to significant and sustained improvements in OHRQoL, with minimal complications across all groups. Both MI diameters (2.5 and 3.0 mm) showed no statistically significant differences in outcomes compared with SIs, supporting their use as reliable alternatives in appropriately selected edentulous patients. Clinical decision-making may therefore be guided primarily by anatomical and patient-related factors rather than by minor variations in implant diameter. FAU - Moftah, Heba M AU - Moftah HM FAU - Al-Saadi, Mohannad H AU - Al-Saadi MH FAU - Darwich, Khaldoun AU - Darwich K LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Int J Prosthodont JT - The International journal of prosthodontics JID - 8900938 SB - IM EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 12:03 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 12:03 [entrez] AID - 7143524 [pii] AID - 10.11607/ijp.9778 [doi] PST - aheadofprint SO - Int J Prosthodont. 2026 Oct 28;0(0):1-21. doi: 10.11607/ijp.9778. PMID- 42949774 OWN - NLM STAT- Publisher LR - 20261028 IS - 1942-4426 (Electronic) IS - 0893-2174 (Linking) VI - 0 IP - 0 DP - 2026 Oct 28 TI - Erosive-Abrasive Effects on Surface, Mechanical, and Optical Properties of 3D-Printed Resin Composites for Prosthetic Applications. PG - 1-30 LID - 10.11607/ijp.9907 [doi] AB - PURPOSE: To evaluate the effects of erosive-abrasive challenges on the microhardness (SH), surface roughness (SR), gloss (Gs), surface morphology, and color stability (DeltaE) of 3D-printed resin composites for use in dental prosthetic restorations. MATERIALS AND METHODS: Rectangular specimens (10x10x2 mm(3); n=10) were fabricated using digital light processing (DLP) 3D printing, post-polymerization (post-curing), polished, and standardized. Specimens were subjected to an erosive-abrasive protocol involving citric acid immersion and simulated toothbrushing. SH, SR, and Gs were measured before and after cycling. Additional specimens were immersed in coffee to assess DeltaE, and surface morphology was analyzed by scanning electron microscopy. Data were statistically analyzed (alpha=0.05). RESULTS: Significant differences in SH were observed among materials at baseline and after cycling (p < 0.001). The nanohybrid resin composite showed the highest initial values but a significant reduction after the challenge, whereas nanosilicate and zirconia-containing composites remained stable. SR did not differ among groups, although only the nanohybrid resin composite showed a significant increase after toothbrushing. Gloss was influenced by material and time, with the nanosilicate resin composite maintaining the highest values after cycling, although all materials exhibited a reduction after the challenge. No differences in DeltaE were observed under a white background, whereas significant differences were found under a black background, with higher variability for the nanosilicate resin composite. CONCLUSION: The response of 3D-printed resin composites to erosive-abrasive challenges was material-dependent. Nanohybrid composites showed greater susceptibility to degradation, whereas zirconia-containing and nanosilicate resin composites demonstrated a more favorable response under all evaluated parameters. These findings highlight the importance of material selection for esthetic outcomes and potential long-term performance in dental prostheses; however, extrapolation to clinical longevity requires further investigation. FAU - Massola Filho, Francisco AU - Massola Filho F FAU - Rodrigues, Mariana Souza AU - Rodrigues MS FAU - Rached, Filipe Abi AU - Rached FA FAU - Rainundo, Sofia Furlanete AU - Rainundo SF FAU - Velo, Marilia Mattar De Amoedo Campos AU - Velo MMAC FAU - Campos, Edson Alves De AU - Campos EA LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Int J Prosthodont JT - The International journal of prosthodontics JID - 8900938 SB - IM EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 12:03 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 12:03 [entrez] AID - 7143479 [pii] AID - 10.11607/ijp.9907 [doi] PST - aheadofprint SO - Int J Prosthodont. 2026 Oct 28;0(0):1-30. doi: 10.11607/ijp.9907. PMID- 42949773 OWN - NLM STAT- Publisher LR - 20261028 IS - 1759-9679 (Electronic) IS - 1759-9660 (Linking) DP - 2026 Oct 28 TI - Development of an eco-friendly sensor manufactured from reused milk cartons and graphene-based conductive ink as substrates. LID - 10.1039/d6ay01428c [doi] AB - This work presents the development of an innovative, disposable, and eco-friendly electrochemical sensor based on a post-consumer milk carton substrate and graphene-based conductive ink. The sensor was fabricated through a simple and cost-effective process, promoting sustainable reuse of urban solid waste while maintaining excellent electrochemical performance. Cyclic voltammetry and square wave voltammetry revealed a reversible redox profile and efficient electron transfer properties, demonstrating the viability of the device for analytical applications. The sensor was applied to the detection of two analytes of high clinical and environmental relevance: L-cysteine (L-Cys), a key biomarker involved in redox balance under pathological conditions, and paraquat (PQ), a highly toxic herbicide widely used in agriculture. For L-Cys, the device showed a linear response in the range of 50.0 to 700.0 micromol L(-1) with a detection limit of 14.6 micromol L(-1), suitable for physiological levels in human serum. For PQ, a linear response was observed in the range of 5.0 to 300.0 micromol L(-1), with a detection limit of 0.21 micromol L(-1), meeting the safety standards established by U.S. regulations for drinking water. The sensor also demonstrated good selectivity in the presence of common interfering species and was successfully applied in real sample analyses, including serum, tap water, and fruit juice. These results highlight the potential of this low-cost and sustainable platform for decentralized and real-time monitoring in clinical diagnostics and environmental safety, aligning with global sustainability goals and promoting circular economy principles. FAU - Blasques, Rodrigo Vieira AU - Blasques RV AUID- ORCID: 0000-0001-7059-3145 AD - Electrochemistry and Biotechnology Laboratory, University of CEUMA - UNICEUMA, Sao Luis, MA, 65065-470, Brazil. blasques@live.com. AD - Multifunctional Nanomaterials Hybrid Laboratory (LABNAHM), Engineering School, Mackenzie Presbyterian University, Sao Paulo, Brazil. FAU - Nascimento Sousa, Amanda Caroline AU - Nascimento Sousa AC AD - Electrochemistry and Biotechnology Laboratory, University of CEUMA - UNICEUMA, Sao Luis, MA, 65065-470, Brazil. blasques@live.com. FAU - Cruz, Glauber AU - Cruz G AUID- ORCID: 0000-0003-0468-553X AD - Processes and Thermochemical Systems Laboratory (LPSisTer), Department of Mechanical Engineering, Federal University of Maranhao (UFMA), Sao Luis, Maranhao, 65085-580, Brazil. FAU - Canevari, Thiago C AU - Canevari TC AUID- ORCID: 0000-0002-4336-8097 AD - Multifunctional Nanomaterials Hybrid Laboratory (LABNAHM), Engineering School, Mackenzie Presbyterian University, Sao Paulo, Brazil. FAU - Barcellos da Rosa, Marcelo AU - Barcellos da Rosa M AD - Federal University of Santa Maria - UFSM, Department of Chemistry, Av. Roraima, 1000, Santa Maria, RS, 97105-900, Brazil. FAU - Mendes Villis, Paulo Cesar AU - Mendes Villis PC AUID- ORCID: 0000-0002-7730-3830 AD - Electrochemistry and Biotechnology Laboratory, University of CEUMA - UNICEUMA, Sao Luis, MA, 65065-470, Brazil. blasques@live.com. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Anal Methods JT - Analytical methods : advancing methods and applications JID - 101519733 SB - IM EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 12:03 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 12:03 [entrez] AID - 10.1039/d6ay01428c [doi] PST - aheadofprint SO - Anal Methods. 2026 Oct 28. doi: 10.1039/d6ay01428c. PMID- 42949772 OWN - NLM STAT- Publisher LR - 20261028 IS - 1533-3450 (Electronic) IS - 1046-6673 (Linking) DP - 2026 Oct 28 TI - Proteinuria, eGFR Decline, and the Pathway to Kidney Failure in Alport Syndrome. LID - 10.1681/ASN.0000001318 [doi] AB - Alport syndrome is a genetic disorder caused by pathogenic variants in COL4A3, COL4A4, or COL4A5, which encode the alpha3, alpha4, and alpha5 chains of type IV collagen. These chains form the collagen alpha3alpha4alpha5(IV) network required for basement membrane function in the kidney, inner ear, and eye. In the kidney, impaired production, assembly, or localization of the alpha3alpha4alpha5 network leads to abnormal glomerular basement membrane (GBM) composition, podocyte dysfunction, proteinuria, glomerulosclerosis, progressive loss of filtration capacity, and kidney failure. End point selection for clinical trials in Alport syndrome requires measures that report disease activity and predict meaningful clinical benefit in trials of feasible size and duration. Proteinuria arises from early stages of Alport pathogenesis: abnormal GBM permeability, podocyte foot-process effacement, podocyte detachment, and glomerulosclerosis. eGFR decline is a later readout of cumulative nephron loss and progression toward kidney failure. This review examines the evidence linking proteinuria and eGFR decline to kidney failure in Alport syndrome, focusing on two contributors to proteinuria: 1) podocyte dysfunction with foot-process effacement, which may be partially modifiable, and 2) proteinuria associated with podocyte loss and glomerulosclerosis, which is less likely to be reversible. The relative contribution of these components may vary with genotype, sex, age, and disease duration. Biopsy-based markers, such as collagen alpha3alpha4alpha5(IV) localization, GBM ultrastructure, podocyte foot-process width, glomerulosclerosis, and tubulointerstitial fibrosis, provide staging information along the disease pathway. The current role of these biopsy markers is strongest for target engagement and pathway modification, because quantitative relationships between changes in these markers and kidney failure risk remain incompletely defined. An end point framework for Alport syndrome should account for genotype, sex, age, baseline proteinuria, baseline eGFR, disease duration, and the expected nonlinear trajectories of proteinuria and eGFR decline. CI - Copyright (c) 2026 by the American Society of Nephrology. FAU - Gale, Daniel P AU - Gale DP AUID- ORCID: 0000-0002-9170-1579 AD - Centre for Kidney and Bladder Health, University College London, UK. FAU - Miner, Jeffrey H AU - Miner JH AUID- ORCID: 0000-0002-1510-8714 AD - Division of Nephrology, Department of Medicine, WashU Medicine, St. Louis, MO, USA. FAU - Rheault, Michelle N AU - Rheault MN AUID- ORCID: 0000-0003-2494-3970 AD - Division of Pediatric Nephrology, Department of Pediatrics, University of Minnesota Medical School, Minneapolis, Minnesota, USA. FAU - Fornoni, Alessia AU - Fornoni A AUID- ORCID: 0000-0002-1313-7773 AD - Peggy and Harold Katz Family Drug Discovery Center and Division of Nephrology and Hypertension, University of Miami, USA. FAU - Lennon, Rachel AU - Lennon R AUID- ORCID: 0000-0001-6400-0227 AD - Manchester Cell-Matrix Centre, Division of Cell-Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, UK. AD - Department of Paediatric Nephrology, Royal Manchester Children's Hospital, Central Manchester University Hospitals NHS Foundation Trust, Manchester Academic Health Centre. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Am Soc Nephrol JT - Journal of the American Society of Nephrology : JASN JID - 9013836 SB - IM EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 12:03 PHST- 2026/07/31 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 12:03 [entrez] AID - 00001751-990000000-01177 [pii] AID - 10.1681/ASN.0000001318 [doi] PST - aheadofprint SO - J Am Soc Nephrol. 2026 Oct 28. doi: 10.1681/ASN.0000001318. PMID- 42949770 OWN - NLM STAT- Publisher LR - 20261028 IS - 2050-7518 (Electronic) IS - 2050-750X (Linking) DP - 2026 Oct 28 TI - Enzyme-functionalized beta-cyclodextrin silicasomes for sequential stroma remodeling and combination chemotherapy in neuroblastoma. LID - 10.1039/d6tb01810f [doi] AB - Neuroblastoma is a high-risk cancer that develops in the nervous system of children under the age of five. One of its main challenges is that it develops a very dense desmoplastic stroma that physically obstructs the penetration of treatments and promotes the malignant capabilities of cancer cells, which rapidly develop resistance to chemotherapeutic agents. This renders traditional treatments ineffective, as the high doses required to overcome these barriers lead to severe off-target toxicities, causing irreversible damage to the patient's developing tissues. To breach this protective microenvironment, we engineered an intelligent, multi-stage nanoplatform consisting of collagenase-functionalized beta-cyclodextrin silicasomes for sequential stroma remodelling and combination chemotherapy. Using extracellular matrix-biomimetic 3D neuroblastoma models, we confirmed that the surface-bound enzymatic action of the nanosystems actively degraded the components of the external stroma, facilitating deep penetration of the silicasomes into the shielded tumor core. Once inside, the nanocarriers selectively targeted cancer cells and their complementation with the beta-cyclodextrin modules drove the co-delivery of complementary chemotherapeutics doxorubicin and paclitaxel, which exhibit high cytotoxicity against solid malignancies but are traditionally limited by issues related to differing pharmacokinetics. Ultimately, these findings provide an in vitro proof-of-concept for addressing stromal barriers in neuroblastoma, offering a strategy that warrants further biocompatibility and in vivo studies to validate its translational potential across stroma-rich solid tumors. FAU - Plaza-Palomo, Guillermo AU - Plaza-Palomo G AUID- ORCID: 0009-0008-7613-3404 AD - Dpto. Materiales y Produccion Aeroespacial, ETSI Aeronautica y del Espacio, Universidad Politecnica de Madrid, 28040 Madrid, Spain. sandra.jfalcao@upm.es. FAU - Calvo-Jimenez, Lara AU - Calvo-Jimenez L AD - Dpto. Materiales y Produccion Aeroespacial, ETSI Aeronautica y del Espacio, Universidad Politecnica de Madrid, 28040 Madrid, Spain. sandra.jfalcao@upm.es. FAU - Jimenez-Falcao, Sandra AU - Jimenez-Falcao S AD - Dpto. Materiales y Produccion Aeroespacial, ETSI Aeronautica y del Espacio, Universidad Politecnica de Madrid, 28040 Madrid, Spain. sandra.jfalcao@upm.es. FAU - Megias, Diego AU - Megias D AD - Advanced Optical Microscopy Unit, Instituto de Salud Carlos III (ISCIII), Madrid, Spain. FAU - Gonzalez-Murillo, Africa AU - Gonzalez-Murillo A AD - Servicio de Hematologia y Oncologia Pediatrica, Hospital Infantil Universitario Nino Jesus, Madrid, Spain. FAU - Ramirez, Manuel AU - Ramirez M AD - Servicio de Hematologia y Oncologia Pediatrica, Hospital Infantil Universitario Nino Jesus, Madrid, Spain. FAU - Baeza, Alejandro AU - Baeza A AUID- ORCID: 0000-0002-9042-8865 AD - Dpto. Materiales y Produccion Aeroespacial, ETSI Aeronautica y del Espacio, Universidad Politecnica de Madrid, 28040 Madrid, Spain. sandra.jfalcao@upm.es. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - J Mater Chem B JT - Journal of materials chemistry. B JID - 101598493 SB - IM EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 11:53 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 11:53 [entrez] AID - 10.1039/d6tb01810f [doi] PST - aheadofprint SO - J Mater Chem B. 2026 Oct 28. doi: 10.1039/d6tb01810f. PMID- 42949768 OWN - NLM STAT- Publisher LR - 20261028 IS - 2168-6602 (Electronic) IS - 0890-1171 (Linking) DP - 2026 Oct 28 TI - The "Best of 2026 List" of Health Promotion Researchers. PG - 8901171261502805 LID - 10.1177/08901171261502805 [doi] AB - Each year the editorial team of the American Journal of Health Promotion selects our "Best of the Year List" of health promotion studies from the prior year. This editorial features the Editor's Picks Awards, the Editor in Chief Awards, the Michael P. O'Donnell Award and the Dorothy Nyswander Award for the best research and writing published in 2026 in this journal. Our criteria for selection includes: whether the study addresses a topic of timely importance in health promotion, the research question is clearly stated and the study methods used are well executed; whether the paper is often cited and downloaded; if the study findings offer a unique contribution to the literature; and if the paper is well-written and enjoyable to read. Awardees in 2026 brought our journal's readers timely perspectives on the role of artificial intelligence in addressing mental health, the role of public policy in supporting caregivers, the pernicious effects of misinformation on our views of research findings and the wonderful contributions story-telling can make in effective health education. This "best of 2026 list" featured studies based in low-income neighborhoods, healthcare delivery systems, workplaces and mental health agencies and includes case studies and qualitative and quantitative research methods that enabled researchers to better explain what helps or hinders well-being. FAU - Terry, Paul E AU - Terry PE AD - The Health Enhancement Research Organization (HERO), Raleigh, NC, USA. LA - eng PT - Editorial DEP - 20261028 PL - United States TA - Am J Health Promot JT - American journal of health promotion : AJHP JID - 8701680 SB - IM OTO - NOTNLM OT - artificial intelligence OT - best practices OT - best science OT - best studies OT - health promotion OT - story-telling EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 11:53 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 11:53 [entrez] AID - 10.1177/08901171261502805 [doi] PST - aheadofprint SO - Am J Health Promot. 2026 Oct 28:8901171261502805. doi: 10.1177/08901171261502805. PMID- 42949767 OWN - NLM STAT- Publisher LR - 20261028 IS - 2198-3844 (Electronic) IS - 2198-3844 (Linking) DP - 2026 Oct 28 TI - Single-Nucleus RNA Sequencing Identifies CREM as a Brake on Myofiber Hypertrophy in Chickens. LID - e78534 [pii] LID - 10.1002/advs.78534 [doi] AB - Myofiber hypertrophy is central to economically important agricultural traits and vertebrate development, yet the underlying cellular and molecular programs remain elusive. Chickens have undergone substantial divergence in muscle growth following decades of artificial selection, making them a unique model for dissecting these mechanisms at single-cell resolution. Here, we performed singlenucleus RNA sequencing on 91 144 nuclei from postnatal chicken pectoralis major. Eight major conserved cell populations were resolved, with temporal dynamics exhibiting divergence between fast-growing broilers and slow-growing layers. A novel terminally differentiated myocyte subpopulation (AK1(+) myocytes) was markedly enriched in slow-growing birds. Analysis revealed that accelerated myoblast expansion and delayed terminal differentiation are primary drivers of myofiber hypertrophy, supported by a pro-growth niche involving IGF-signaling FAPs and macrophage-myogenic crosstalk. Further comprehensive analyses identified CREM as a master repressor of myofiber hypertrophy. Mechanistically, CREM directly binds and transcriptionally activates the JUN promoter, and both factors cooperatively sustain the expression of growth inhibitors MSTN, FBXO32, and TRIM63. In vivo and in vitro functional validation confirmed that CREM deletion promotes myofiber hypertrophy, increasing pectoralis major weight and percent by 29.96% and 20.50%, respectively. This study uncovers CREM as a novel brake on myofiber hypertrophy, offering fundamental insights into vertebrate skeletal muscle development. CI - (c) 2026 The Author(s). Advanced Science published by Wiley-VCH GmbH. FAU - Gu, Shuang AU - Gu S AD - National Engineering Laboratory for Animal Breeding, Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China. AD - Department of Animal Genetics and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China. FAU - Zhao, Xiyu AU - Zhao X AD - State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China. FAU - Jiang, Zheyi AU - Jiang Z AD - National Engineering Laboratory for Animal Breeding, Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China. FAU - Wen, Chaoliang AU - Wen C AUID- ORCID: 0000-0002-0514-2847 AD - National Engineering Laboratory for Animal Breeding, Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China. FAU - Wang, Chaoyi AU - Wang C AD - National Engineering Laboratory for Animal Breeding, Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China. FAU - Wang, Qiulian AU - Wang Q AD - National Engineering Laboratory for Animal Breeding, Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China. FAU - Huang, Qiang AU - Huang Q AD - National Engineering Laboratory for Animal Breeding, Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China. FAU - Lan, Fangren AU - Lan F AD - National Engineering Laboratory for Animal Breeding, Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China. FAU - Wu, Shenghui AU - Wu S AD - LC-Bio Technology Co. Ltd., Hangzhou, China. FAU - Li, Junying AU - Li J AUID- ORCID: 0000-0001-9842-8723 AD - National Engineering Laboratory for Animal Breeding, Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China. FAU - Sun, Congjiao AU - Sun C AUID- ORCID: 0000-0002-9673-9533 AD - National Engineering Laboratory for Animal Breeding, Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China. FAU - Yin, Huadong AU - Yin H AD - State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China. FAU - Yang, Ning AU - Yang N AUID- ORCID: 0000-0001-5772-3320 AD - National Engineering Laboratory for Animal Breeding, Department of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China. LA - eng GR - 2022YFF1000200/National Key Research and Development Program of China/ GR - 00109015/2115 Talent Development Program of China Agricultural University/ PT - Journal Article DEP - 20261028 PL - Germany TA - Adv Sci (Weinh) JT - Advanced science (Weinheim, Baden-Wurttemberg, Germany) JID - 101664569 SB - IM PMC - PMC13715926 OTO - NOTNLM OT - chicken OT - myofiber hypertrophy OT - single-nucleus RNA sequencing OT - skeletal muscle development OT - transcription factor COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 PMCR- 2026/10/28 CRDT- 2026/10/28 11:53 PHST- 2026/10/16 00:00 [revised] PHST- 2026/07/06 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 11:53 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e78534 [pii] AID - ADVS78534 [pii] AID - 10.1002/advs.78534 [doi] PST - aheadofprint SO - Adv Sci (Weinh). 2026 Oct 28:e78534. doi: 10.1002/advs.78534. PMID- 42949757 OWN - NLM STAT- Publisher LR - 20261028 IS - 2040-3372 (Electronic) IS - 2040-3364 (Linking) DP - 2026 Oct 28 TI - Polyoxoniobate-hybridized gold nanoparticles for the electrooxidation of glycerol to value-added C3 products. LID - 10.1039/d6nr03911a [doi] AB - Au nanoparticle catalysts are benchmark materials for the electrooxidation of glycerol under alkaline conditions; however, achieving high selectivity toward value-added C3 products remains a fundamental challenge. Herein, we present a strategy to regulate the electronic structure of Au nanoparticles, thereby controlling product selectivity during glycerol electrooxidation through hybridization of Au nanoparticles with polyoxometalate anions. Hybridization with a highly negatively charged polyoxoniobate cluster Nb6 ([Nb(6)O(19)](8-)) enhances C3 selectivity under strongly alkaline conditions, with Nb species retaining Nb6-like local structures. Notably, Nb6 bearing Ba(2+) as a countercation enhanced C3 selectivity more effectively than the corresponding Mg(2+) salt, achieving a C3 selectivity of 76% at +1.15 V(RHE). Nb6 hybridization alters the electrochemical behavior of the Au surface, potentially modulating the electronic states of Au, weakening the adsorption strength of reaction intermediates, and thereby directing the reaction pathway toward C3 products. Furthermore, this electronic effect is more pronounced for the Ba(2+) salt than for the Mg(2+) salt, consistent with the observed glycerol electrooxidation performance. These findings demonstrate that hybridization of Au catalysts with alkaline-stable polyoxometalates provides a powerful and generalizable approach for engineering catalyst-electrolyte interfaces and enhancing catalytic performance in alkaline electrocatalytic reactions. FAU - Akino, Shimon AU - Akino S AD - Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. FAU - Kawakami, Kimitake AU - Kawakami K AD - Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. FAU - Yonesato, Kentaro AU - Yonesato K AUID- ORCID: 0000-0001-9143-6691 AD - Department of Advanced Materials Science, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwa-no-ha, Kashiwa-shi, Chiba 277-8563, Japan. ksuzuki@appchem.t.u-tokyo.ac.jp. FAU - Kikkawa, Soichi AU - Kikkawa S AUID- ORCID: 0000-0002-8193-6374 AD - Department of Chemistry, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji-shi, Tokyo 192-0397, Japan. FAU - Yamazoe, Seiji AU - Yamazoe S AUID- ORCID: 0000-0002-8382-8078 AD - Department of Chemistry, Graduate School of Science, Tokyo Metropolitan University, 1-1 Minami-Osawa, Hachioji-shi, Tokyo 192-0397, Japan. FAU - Kaneko, Takuma AU - Kaneko T AD - Japan Synchrotron Radiation Research, Institute (JASRI), 1-1-1 Kouto, Sayo-gun, Sayo-cho, Hyogo 679-5198, Japan. FAU - Uruga, Tomoya AU - Uruga T AD - Japan Synchrotron Radiation Research, Institute (JASRI), 1-1-1 Kouto, Sayo-gun, Sayo-cho, Hyogo 679-5198, Japan. FAU - Honjo, Yoshiaki AU - Honjo Y AD - RIKEN SPring-8 Center, RIKEN, 1-1-1 Kouto, Sayo-gun, Sayo-cho, Hyogo 679-5148, Japan. FAU - Hatsui, Takaki AU - Hatsui T AUID- ORCID: 0000-0001-8144-3484 AD - RIKEN SPring-8 Center, RIKEN, 1-1-1 Kouto, Sayo-gun, Sayo-cho, Hyogo 679-5148, Japan. FAU - Harano, Koji AU - Harano K AUID- ORCID: 0000-0001-6800-8023 AD - Center for Basic Research on Materials, National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba-shi, Ibaraki 305-0044, Japan. AD - Research Center for Autonomous Systems Materialogy (ASMat), Institute of Integrated Research, Institute of Science Tokyo, 4259 Nagatsuta-cho, Midori-ku, Yokohama-shi, Kanagawa 226-8501, Japan. FAU - Yamaguchi, Kazuya AU - Yamaguchi K AUID- ORCID: 0000-0002-7661-4936 AD - Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. FAU - Suzuki, Kosuke AU - Suzuki K AUID- ORCID: 0000-0002-8123-1462 AD - Department of Applied Chemistry, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. AD - Department of Advanced Materials Science, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwa-no-ha, Kashiwa-shi, Chiba 277-8563, Japan. ksuzuki@appchem.t.u-tokyo.ac.jp. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Nanoscale JT - Nanoscale JID - 101525249 SB - IM EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 11:35 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 11:35 [entrez] AID - 10.1039/d6nr03911a [doi] PST - aheadofprint SO - Nanoscale. 2026 Oct 28. doi: 10.1039/d6nr03911a. PMID- 42949745 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2574-3805 (Electronic) IS - 2574-3805 (Linking) VI - 9 IP - 10 DP - 2026 Oct 1 TI - Organ Preservation With Watch and Wait in Patients With Rectal Cancer. PG - e2640267 LID - 10.1001/jamanetworkopen.2026.40267 [doi] LID - e2640267 AB - IMPORTANCE: With growing evidence and evolving guidelines, organ preservation with watch and wait for stage II to III rectal cancer after neoadjuvant therapy is increasingly implemented. However, the true frequency of watch-and-wait use is unknown. OBJECTIVE: To estimate rates of intentional organ preservation with watch and wait by evaluating secular trends across age groups. DESIGN, SETTING, AND PARTICIPANTS: This retrospective cohort study used data from the National Cancer Database (NCDB), with analyses conducted in September 2025. Patients with stage II to III rectal cancer diagnosed between 2006 and 2023 who received neoadjuvant chemoradiation in Commission on Cancer-accredited hospitals participating in the NCDB across the US were included. EXPOSURE: Age, year of diagnosis, and treatment strategy (proctectomy vs nonsurgical management). MAIN OUTCOMES AND MEASURES: The primary outcome consisted of watch-and-wait rates, defined as excess nonsurgical management greater than baseline. To account for nonsurgical management unrelated to the decision for watch and wait, joinpoint regression estimated baseline rates of nonsurgical management and annual percentage changes. A secondary analysis examined associations of age and year with watch-and-wait rates using multivariable logistic regression. RESULTS: Among 101 001 patients with rectal cancer (median [IQR] age, 60 [51-69] years; 37 957 female [37.58%]), 84 324 underwent surgery and 16 677 did not. Baseline rates of nonsurgical management remained stable through 2014. Joinpoints occurred in 2014 and 2019, with annual percentage changes of -0.45% (95% CI, -2.26% to 0.78%; P = .42) in 2006 to 2014, 9.83% (95% CI, 4.66% to 13.73%; P = .001) in 2015 to 2018, and 21.71% (95% CI, 17.74% to 29.57%; P < .001) in 2019 to 2023. Baseline nonsurgical management increased with age, from 1331 of 19 785 patients younger than 50 years (6.73%) to 1748 of 6210 patients aged 80 years and older (28.15%). Earlier adoption occurred in older patients; the group aged 80 years and older surpassed 5-percentage point excess watch and wait by 2015 (33.78% annual rate vs 28.15% baseline rate [5.63-percentage point excess]) and 10% by 2017 (39.56% annual rate vs 28.15% baseline rate [11.41-percentage point excess]), compared with 2019 (12.88% annual rate vs 6.73% baseline rate [6.15-percentage point excess]) and 2021 (20.75% annual rate vs 6.73% baseline rate [14.02%] excess) in patients younger than 50 years. Overall, watch and wait increased from 140 of 6101 patients (2.30%) in 2015 to 2068 of 6280 patients (32.94%) in 2023. Increasing year (odds ratio for 2023 vs 2006, 6.23; 95% CI, 5.48 to 7.09) and older age (odds ratio for ages >/=80 vs <50 years, 4.25; 95% CI, 3.98 to 4.54) were independently associated with nonsurgical management (both P < .001). CONCLUSIONS AND RELEVANCE: In this study, watch-and-wait rates increased across all age groups, with a marked acceleration after 2019 suggesting evidence-concordant adoption of the watch-and-wait approach. This underscores the need for careful patient selection, accurate clinical response assessment, and rigorous surveillance monitoring to ensure safe oncologic outcomes. FAU - Keeling, Stephanie S AU - Keeling SS AD - Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center, Houston. AD - Department of Surgery, Baylor College of Medicine, Houston, Texas. FAU - Behman, Ramy AU - Behman R AD - Department of Colon and Rectal Surgery, The University of Texas MD Anderson Cancer Center, Houston. FAU - Hu, Chung-Yuan AU - Hu CY AD - Department of Colon and Rectal Surgery, The University of Texas MD Anderson Cancer Center, Houston. FAU - Lin, John K AU - Lin JK AD - Department of Health Services Research, The University of Texas MD Anderson Cancer Center, Houston. AD - Department of Genitourinary Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston. FAU - Manisundaram, Naveen AU - Manisundaram N AD - Department of Surgery, Baylor College of Medicine, Houston, Texas. FAU - Moon, Jeongyoon AU - Moon J AD - Department of Colon and Rectal Surgery, The University of Texas MD Anderson Cancer Center, Houston. FAU - Bednarski, Brian K AU - Bednarski BK AD - Department of Colon and Rectal Surgery, The University of Texas MD Anderson Cancer Center, Houston. FAU - Konishi, Tsuyoshi AU - Konishi T AD - Department of Colon and Rectal Surgery, The University of Texas MD Anderson Cancer Center, Houston. FAU - Marincola Smith, Paula AU - Marincola Smith P AD - Department of Colon and Rectal Surgery, The University of Texas MD Anderson Cancer Center, Houston. FAU - Smith, J Joshua AU - Smith JJ AD - Department of Colon and Rectal Surgery, The University of Texas MD Anderson Cancer Center, Houston. FAU - White, Michael G AU - White MG AD - Department of Colon and Rectal Surgery, The University of Texas MD Anderson Cancer Center, Houston. FAU - Chang, George J AU - Chang GJ AD - Department of Colon and Rectal Surgery, The University of Texas MD Anderson Cancer Center, Houston. AD - Department of Health Services Research, The University of Texas MD Anderson Cancer Center, Houston. LA - eng PT - Journal Article DEP - 20261001 PL - United States TA - JAMA Netw Open JT - JAMA network open JID - 101729235 SB - IM CIN - doi: 10.1001/jamanetworkopen.2026.40202 MH - Humans MH - *Rectal Neoplasms/therapy/pathology MH - Female MH - *Watchful Waiting/statistics & numerical data MH - Retrospective Studies MH - Male MH - Middle Aged MH - Aged MH - *Organ Sparing Treatments/statistics & numerical data/methods MH - Neoadjuvant Therapy MH - United States MH - Proctectomy/statistics & numerical data MH - Neoplasm Staging PMC - PMC13717419 COIS- Conflict of Interest Disclosures: Dr Lin reported receiving grants from the National Institutes of Health outside the submitted work. Dr Marincola Smith reported receiving personal fees from Sentinel BioTherapeutics, Intuitive Surgical, Now UptoDate, Iota, Guardant Health, Foundation Medicine, Johnson & Johnson, Urogen, Regeneron, GSK, Gerson Lehrman Group, Agenus, BioNTech, and Clario and travel support from Artidis outside the submitted work. No other disclosures were reported. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 11:34 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 11:34 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 2855919 [pii] AID - zoi261093 [pii] AID - 10.1001/jamanetworkopen.2026.40267 [doi] PST - epublish SO - JAMA Netw Open. 2026 Oct 1;9(10):e2640267. doi: 10.1001/jamanetworkopen.2026.40267. PMID- 42949744 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2574-3805 (Electronic) IS - 2574-3805 (Linking) VI - 9 IP - 10 DP - 2026 Oct 1 TI - Bias-Adjusted Prevalence of Chronic Traumatic Encephalopathy Among Deceased National Football League Players. PG - e2640884 LID - 10.1001/jamanetworkopen.2026.40884 [doi] LID - e2640884 AB - IMPORTANCE: The majority of knowledge regarding chronic traumatic encephalopathy (CTE) in National Football League (NFL) players is derived from autopsy-based studies on voluntarily donated brains. Because these cohorts are highly vulnerable to selection bias (specifically registry bias), population-level prevalence of CTE among former NFL players is unknown, limiting understanding of the impact of this disease. OBJECTIVE: To estimate the selection-bias adjusted prevalence of CTE neuropathology in deceased NFL players. DESIGN, SETTING, AND PARTICIPANTS: This population-based cross-sectional study used Understanding Neurologic Injury and Traumatic Encephalopathy (UNITE) brain bank data combined with National Death Index records on former NFL players. Both brain donor and nondonor NFL players who died between January 2008 and December 2023 were included. Data were analyzed from November 2025 to April 2026. EXPOSURE: Playing in at least 1 regular or postseason NFL game. MAIN OUTCOMES AND MEASURES: The main outcomes of this study were bias-adjusted prevalence of CTE neuropathology and all-cause dementia among the target population, both of which were measured in donors prior to this study. A cognitive symptom-related brain donation selection ratio (probability of donation given CTE divided by the probability of donation given no CTE) was estimated using Poisson regression and was applied to adjust donor-observed prevalence of CTE neuropathology at death and all-cause dementia during life. The prevalence estimates were adjusted for bias using the estimated selection ratio. RESULTS: The cohort consisted of 2362 deceased former NFL players (mean [SD] years played, 5.1 [3.9]; 971 [41.1%] aged >/=80 years at death), of whom 396 were brain donors and 1966 were nondonors. Of 387 donors, 362 (93.5%) had CTE. The median selection ratio was 4.97 (95% CI, 4.72-5.26). Bias-adjusted population prevalence of CTE at death was 74.5% (95% CI, 69.6%-78.3%), with a conservative lower-bound analysis estimating 63.7% (57.2%-68.9%). Bias-adjusted population prevalence of all-cause dementia history during life was 27.2% (95% CI, 22.6%-31.6%). CONCLUSIONS AND RELEVANCE: The findings of this cross-sectional study suggest that selection bias materially inflates donor-based prevalence estimates but is unlikely to fully explain the high burden of CTE neuropathology among this population. Further research is required to understand the clinical impact of this disease. FAU - Gallini, Julia AU - Gallini J AD - Department of Biostatistics, Boston University School of Public Health, Boston, Massachusetts. FAU - Alosco, Michael L AU - Alosco ML AD - Departments of Anatomy & Neurobiology, Chobanian & Avedisian School of Medicine, Boston, Massachusetts. AD - Boston University Alzheimer's Disease Research Center and CTE Center, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts. FAU - Abdolmohammadi, Bobak AU - Abdolmohammadi B AD - Boston University Alzheimer's Disease Research Center and CTE Center, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts. FAU - Weuve, Jennifer AU - Weuve J AD - Department of Epidemiology, Boston University School of Public Health, Boston, Massachusetts. FAU - McKee, Ann C AU - McKee AC AD - Boston University Alzheimer's Disease Research Center and CTE Center, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts. AD - Department of Neurology, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts. AD - Department of Pathology and Laboratory Medicine, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts. FAU - Stein, Thor D AU - Stein TD AD - Boston University Alzheimer's Disease Research Center and CTE Center, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts. AD - Department of Pathology and Laboratory Medicine, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts. FAU - Stern, Robert A AU - Stern RA AD - Departments of Anatomy & Neurobiology, Chobanian & Avedisian School of Medicine, Boston, Massachusetts. AD - Boston University Alzheimer's Disease Research Center and CTE Center, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts. AD - Department of Neurology, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts. AD - Department of Neurosurgery, Chobanian & Avedisian School of Medicine, Boston, Massachusetts. FAU - Daneshvar, Daniel H AU - Daneshvar DH AD - Department of Physical Medicine and Rehabilitation, Spaulding Rehabilitation Hospital, Charlestown, Massachusetts. FAU - Mez, Jesse AU - Mez J AD - Boston University Alzheimer's Disease Research Center and CTE Center, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts. AD - Department of Neurology, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts. FAU - Nowinski, Christopher AU - Nowinski C AD - Boston University Alzheimer's Disease Research Center and CTE Center, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts. AD - Concussion & CTE Foundation, Boston, Massachusetts. FAU - Tripodis, Yorghos AU - Tripodis Y AD - Department of Biostatistics, Boston University School of Public Health, Boston, Massachusetts. AD - Boston University Alzheimer's Disease Research Center and CTE Center, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts. LA - eng PT - Journal Article DEP - 20261001 PL - United States TA - JAMA Netw Open JT - JAMA network open JID - 101729235 SB - IM MH - Humans MH - *Chronic Traumatic Encephalopathy/epidemiology MH - *Football/injuries/statistics & numerical data MH - Male MH - Cross-Sectional Studies MH - Prevalence MH - United States/epidemiology MH - Middle Aged MH - Adult MH - Aged MH - Bias MH - *Athletes/statistics & numerical data PMC - PMC13717411 COIS- Conflict of Interest Disclosures: Dr Alosco reported receiving grants from the Department of Defense and Life Molecular Imaging Inc, honorarium from the Michael J Fox Foundation Inc, and royalties from Oxford University Press (textbook editor) outside the submitted work. Dr Weuve reported receiving personal fees from the Alzheimer's Association (writing of facts and figures report) outside the submitted work. Dr Stein reported receiving grants from the US Department of Veterans Affairs outside the submitted work. Dr Stern reported receiving personal fees from Psychological Assessment Resources (royalties from published neuropsychological tests) and Wolters Kluwer (royalties for authorship of the UpToDate topic of chronic traumatic encephalopathy) outside the submitted work. Dr Daneshvar reported having stock options with StataDx and Solius and receiving personal fees from Medicolegal (expert testimony) and Eli Lilly and Co outside the submitted work. Dr Mez reported receiving grants from the Department of Defense and honoraria from Imperial College London and the Lou Ruvo Center for Brain Health outside the submitted work. Dr Nowinski reported receiving travel support from the National Football League, NFL Players Association, WWE, AEW, and Total Nonstop Action; being a volunteer advisor for the NFL Players Association; receiving personal fees from Lilly and the Players Advocacy Committee for the NFL Concussion Settlement; and having stock options with Oxeia Biopharmaceuticals and StataDx outside the submitted work. Dr Tripodis reported receiving personal fees from the American Medical Association outside the submitted work. No other disclosures were reported. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 11:34 PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 11:34 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 2855918 [pii] AID - zoi261108 [pii] AID - 10.1001/jamanetworkopen.2026.40884 [doi] PST - epublish SO - JAMA Netw Open. 2026 Oct 1;9(10):e2640884. doi: 10.1001/jamanetworkopen.2026.40884. PMID- 42949737 OWN - NLM STAT- Publisher LR - 20261028 IS - 2168-6084 (Electronic) IS - 2168-6068 (Print) IS - 2168-6068 (Linking) DP - 2026 Oct 28 TI - Targeted and Immune-Based Therapies in Cutaneous T-Cell Lymphomas: A Systematic Review and Meta-Analysis. LID - 10.1001/jamadermatol.2026.4263 [doi] LID - e264263 AB - IMPORTANCE: Advanced cutaneous T-cell lymphomas (CTCLs), including mycosis fungoides (MF) and Sezary syndrome (SS), are associated with substantial morbidity and limited durable treatment options. Although several targeted and immune-based therapies have emerged, their comparative efficacy, durability, and safety have not been comprehensively synthesized. OBJECTIVE: To evaluate the efficacy and safety of targeted and immune-based monotherapies for CTCL and characterize subtype-specific outcomes in MF and SS. DATA SOURCES: MEDLINE, Embase, and CENTRAL databases were searched from inception to April 10, 2026. STUDY SELECTION: Studies reporting efficacy or safety outcomes in patients with CTCL treated with targeted or immune-based monotherapy were included. Two reviewers independently screened studies and extracted data. DATA EXTRACTION AND SYNTHESIS: Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) reporting guidelines were followed and risk of bias was assessed using the Joanna Briggs Institute tool. Random-effects meta-analyses were performed to estimate pooled overall response rates (ORRs) with 95% CIs. MAIN OUTCOMES AND MEASURES: Pooled ORR; secondary outcomes were duration of response, progression-free survival, time to response, treatment-related adverse events (AEs), grade 3 or greater AEs, treatment discontinuations, recurrence, and subtype-specific response rates. RESULTS: The analysis included 41 studies comprising 1660 patients with a mean (SD) age of 61.6 (12.6) years; of the 1460 with reported sex, 647 were female (44.3%) and 813 male (55.7%) individuals. Pooled ORRs varied across therapeutic cohorts, led by brentuximab vedotin (69%; 95% CI, 62%-75%; n = 510), interferon alfa (68%; 95% CI, 32%-90%; n = 59), alemtuzumab (65%; 95% CI, 45%-80%; n = 96), mogamulizumab (45%; 95% CI, 35%-56%; n = 514), denileukin diftitox (39%; 95% CI, 33%-46%; n = 341), and pembrolizumab (34%; 95% CI, 20%-51%; n = 33). Among MF cohorts, pooled ORRs ranged from 38% to 66% across evaluated therapies. Among SS cohorts, pooled ORRs ranged from 60% to 74% across evaluated therapies. Sample-size-weighted means of study-reported median duration of response ranged from 4.7 to 9.4 months across reported therapies, while median progression-free survival ranged from 14.4 to 23.3 months. Grade 3 or greater AE rates varied across therapies, ranging from 15% (95% CI, 11%-21%) with mogamulizumab to 26% (95% CI, 7%-64%) with denileukin diftitox. CONCLUSIONS AND RELEVANCE: In this systematic review and meta-analysis, targeted and immune-based therapies demonstrated clinically meaningful activity in CTCL, although important differences existed in response rates, response durability, and toxic effect profiles across therapeutic cohorts. Therapeutic activity also varied according to disease subtype, highlighting the importance of individualized treatment selection. These findings support incorporating disease subtype, anticipated durability of response, and toxicity considerations into treatment decision-making for patients with CTCL. FAU - Bai, Jia Qi Adam AU - Bai JQA AD - Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada. FAU - Wen, Sherilyn AU - Wen S AD - Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada. FAU - Chu, Nicole AU - Chu N AD - Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada. FAU - Geng, Ryan S Q AU - Geng RSQ AD - Division of Dermatology, Department of Medicine, University of Toronto, Toronto, Ontario, Canada. FAU - Gniadecki, Robert AU - Gniadecki R AD - Division of Dermatology, Faculty of Medicine, University of Alberta, Edmonton, Alberta, Canada. FAU - Piguet, Vincent AU - Piguet V AD - Division of Dermatology, Department of Medicine, Women's College Hospital, Toronto, Ontario, Canada. AD - Division of Dermatology, Department of Medicine, University of Toronto, Toronto, Ontario, Canada. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - JAMA Dermatol JT - JAMA dermatology JID - 101589530 SB - IM PMC - PMC13717453 COIS- Conflict of Interest Disclosures: Dr Gniadecki reported advisory board membership and honoraria from Kyowa Kirin during the conduct of the study; advisory board membership and honoraria from AbbVie; institutional grants from UCB outside the submitted work. Dr Piguet reported grants from AbbVie, Eli Lilly, Incyte, Jassen, LEO Pharma, Amgen, Arcutis Canada, Novartis, Organon, Pfizer, Sanofi, and UCB; and receiving payment or honorarium for speaking engagements from Sanofi China; advisory board membership for LEO Pharma, Incyte, Novartis, and UCB, all outside the submitted work. No other disclosures were reported. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 PMCR- 2027/10/28 CRDT- 2026/10/28 11:34 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 11:34 [entrez] PHST- 2027/10/28 00:00 [pmc-release] AID - 2855847 [pii] AID - doi260052 [pii] AID - 10.1001/jamadermatol.2026.4263 [doi] PST - aheadofprint SO - JAMA Dermatol. 2026 Oct 28:e264263. doi: 10.1001/jamadermatol.2026.4263. PMID- 42949711 OWN - NLM STAT- Publisher LR - 20261028 IS - 1029-2330 (Electronic) IS - 1026-7158 (Linking) DP - 2026 Oct 28 TI - Novel Benzimidazole Linked Isoxazoline Derivatives: Molecular Docking, Synthesis, and Pharmacological Evaluation for Anti-Ulcer and Anti-Histaminic Activities. PG - 1-38 LID - 10.1080/1061186X.2026.2756483 [doi] AB - Gastric acid and pepsin damage the GI tract lining in peptic ulcer disease. It affects many people worldwide, especially in developing countries. H1 antihistamines relieve allergy symptoms by blocking H1 receptors, which histamine binds. We designed novel benzimidazole-linked isoxazoline derivatives, molecular docking was carried out to study their possible target interaction with the anti-ulcer associated target 7W48 and H1 antihistaminic target 8X5Y, and synthesized 1-hydroxyethyl)-1H-benzimidazole by condensation of o-phenylenediamine with lactic acid. Claisen-Schmidt condensation of ketone derivatives with substituted aromatic aldehydes produced chalcones. Benzimidazole-linked isoxazoline derivatives (6a-6h) were produced by hydroxylamine hydrochloride cyclization. The ligands with the highest docking scores were selected for biological target evaluation (6d: -7.66 and 6e: -8.62, respectively). In ethanol-induced gastric ulcer models, compound 6d showed dose-dependent gastroprotection, with ulcer indices of 4.227 +/- 0.110 (200 mg/kg) and 3.523 +/- 0.095 (400 mg/kg) exhibiting 27.59% and 39.64% inhibition, respectively, but less effective than omeprazole (1.380 +/- 0.046; 76.36% In an ex vivo study, compound 6e showed concentration-dependent inhibition (20.700 +/- 0.990%-73.800 +/- 0.849% at 200-400 mg/kg), but less than Mizolastine (80.700 +/- 0.849% at 1.6 mug/ml). Compound 6d had dose-dependent antiulcer activity. The concentration-dependent anti-histaminic activity of compound 6e was significant. Standard drugs (omeprazole and mizolastine) were stronger than both compounds. These derivatives showed promising gastroprotective and antihistaminic properties and can be used as lead for further optimization and development. The combined molecular docking and biological evaluation results suggest that compounds 6d and 6e can be further explored for anti-ulcer and antihistaminic activities, respectively. Nevertheless, to confirm their suitability for further drug development studies, experimental validation of their ADME-related properties, toxicity predictions, and proposed molecular mechanisms is required. FAU - H G, Yathish AU - H G Y AD - Department of Pharmaceutical Chemistry, Sri Adichunchanagiri College of Pharmacy, Adichunchanagiri University, B G Nagara, Karnataka, India-571448. FAU - C, Manojmouli AU - C M AD - Department of Pharmaceutical Chemistry, Sri Adichunchanagiri College of Pharmacy, Adichunchanagiri University, B G Nagara, Karnataka, India-571448. FAU - Y M, Yashavanth AU - Y M Y AD - Department of Pharmaceutical Chemistry, Sri Adichunchanagiri College of Pharmacy, Adichunchanagiri University, B G Nagara, Karnataka, India-571448. FAU - B V, Prasad AU - B V P AD - Department of Pharmaceutical Chemistry, Sri Adichunchanagiri College of Pharmacy, Adichunchanagiri University, B G Nagara, Karnataka, India-571448. FAU - Pasha, T Yunus AU - Pasha TY AD - Department of Pharmaceutical Chemistry, Sri Adichunchanagiri College of Pharmacy, Adichunchanagiri University, B G Nagara, Karnataka, India-571448. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - J Drug Target JT - Journal of drug targeting JID - 9312476 SB - IM OTO - NOTNLM OT - Anti-ulcer OT - Antihistamine OT - Benzimidazole OT - Molecular Docking EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 11:33 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 11:33 [entrez] AID - 10.1080/1061186X.2026.2756483 [doi] PST - aheadofprint SO - J Drug Target. 2026 Oct 28:1-38. doi: 10.1080/1061186X.2026.2756483. PMID- 42949705 OWN - NLM STAT- Publisher LR - 20261028 IS - 1460-4744 (Electronic) IS - 0306-0012 (Print) IS - 0306-0012 (Linking) DP - 2026 Oct 28 TI - Label-free Raman spectroscopy for cancer therapy response: from methodological framework to clinical translation. LID - 10.1039/d6cs00966b [doi] AB - The early detection of therapy-induced biomolecular changes and the prediction of therapeutic response remain major challenges in oncology. Current clinical methods often assess treatment response through macroscopic or morphological endpoints, whereas molecular alterations associated with sensitivity, resistance, or recurrence may arise earlier and remain undetected. Raman spectroscopy offers a label-free and non-destructive approach to probe the intrinsic biochemical composition of cells, tissues, and biofluids, providing spectral and spatial information that can support therapy response prediction and longitudinal monitoring. This review discusses Raman-based technologies for cancer therapy assessment, from preclinical models to emerging clinical applications. We first outline the clinical need for rapid, non-destructive, and molecularly informative tools, considering tumor heterogeneity, therapy resistance, the tumor microenvironment, and current standards for response evaluation. We then provide a methodological framework for selecting Raman modalities, including spontaneous Raman (SR) spectroscopy, surface-enhanced Raman spectroscopy (SERS), stimulated Raman spectroscopy (SRS), and coherent anti-Stokes Raman spectroscopy (CARS), together with key considerations on acquisition strategies, multimodal integration, experimental design, preprocessing, feature analysis, and machine learning. We summarize Raman spectral biomarkers associated with therapy-induced changes in nucleic acids, proteins, lipids, metabolites, extracellular matrix components, and anticancer drugs, and critically examine studies using Raman spectroscopy to predict or monitor responses to major therapeutic approaches. Finally, we discuss barriers to clinical translation, including acquisition speed, inter-instrument reproducibility, preclinical model limitations, regulatory requirements, workflow integration, clinical trials, and prospective validation. Overall, Raman spectroscopy emerges as a promising platform for molecular phenotyping in precision oncology, provided that future studies progress toward standardized, clinically validated, and actionable workflows. FAU - Alcolea-Rodriguez, Victor AU - Alcolea-Rodriguez V AUID- ORCID: 0000-0002-2392-0817 AD - CNR-Institute for Photonics and Nanotechnologies (CNR-IFN), P.zza Leonardo Da Vinci 32, 20133 Milan, Italy. victor.alcolea@polimi.it. AD - Department of Physics, Politecnico di Milano, P.zza Leonardo da Vinci 32, 20133 Milan, Italy. FAU - Bresci, Arianna AU - Bresci A AUID- ORCID: 0000-0003-3022-8834 AD - Department of Physics, Politecnico di Milano, P.zza Leonardo da Vinci 32, 20133 Milan, Italy. AD - Laser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA. FAU - Muhlig, Anna AU - Muhlig A AUID- ORCID: 0009-0001-8278-6494 AD - Clinical Biophotonics, Department of Otorhinolaryngology, Jena University Hospital, 07747 Jena, Germany. AD - Comprehensive Cancer Center Central Germany, 07747 Jena, Germany. FAU - Agrimi, Chiara AU - Agrimi C AUID- ORCID: 0009-0009-4710-0520 AD - CNR-Institute for Photonics and Nanotechnologies (CNR-IFN), P.zza Leonardo Da Vinci 32, 20133 Milan, Italy. victor.alcolea@polimi.it. AD - Department of Physics, Politecnico di Milano, P.zza Leonardo da Vinci 32, 20133 Milan, Italy. FAU - Morasso, Carlo AU - Morasso C AUID- ORCID: 0000-0001-9185-0198 AD - Laboratory of Nanomedicine, Istituti Clinici Scientifici Maugeri IRCCS, Pavia, Italy. FAU - Polli, Dario AU - Polli D AUID- ORCID: 0000-0002-6960-5708 AD - CNR-Institute for Photonics and Nanotechnologies (CNR-IFN), P.zza Leonardo Da Vinci 32, 20133 Milan, Italy. victor.alcolea@polimi.it. AD - Department of Physics, Politecnico di Milano, P.zza Leonardo da Vinci 32, 20133 Milan, Italy. FAU - Guntinas-Lichius, Orlando AU - Guntinas-Lichius O AUID- ORCID: 0000-0001-9671-0784 AD - Clinical Biophotonics, Department of Otorhinolaryngology, Jena University Hospital, 07747 Jena, Germany. AD - Department of Otorhinolaryngology, Jena University Hospital, 07747 Jena, Germany. FAU - Vanna, Renzo AU - Vanna R AUID- ORCID: 0000-0001-6218-8393 AD - CNR-Institute for Photonics and Nanotechnologies (CNR-IFN), P.zza Leonardo Da Vinci 32, 20133 Milan, Italy. victor.alcolea@polimi.it. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - Chem Soc Rev JT - Chemical Society reviews JID - 0335405 SB - IM PMC - PMC13715935 COIS- There are no conflicts to declare. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 PMCR- 2026/10/28 CRDT- 2026/10/28 11:33 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 11:33 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - d6cs00966b [pii] AID - 10.1039/d6cs00966b [doi] PST - aheadofprint SO - Chem Soc Rev. 2026 Oct 28. doi: 10.1039/d6cs00966b. PMID- 42949671 OWN - NLM STAT- Publisher LR - 20261028 IS - 1460-2709 (Electronic) IS - 1369-3786 (Linking) DP - 2026 Oct 28 TI - Human infections caused by filamentous basidiomycetes: A global systematic review. LID - myag115 [pii] LID - 10.1093/mmy/myag115 [doi] AB - Filamentous basidiomycetes are increasingly recognized as causes of diverse human disease, yet knowledge of their clinical spectrum, diagnosis, treatment, antifungal susceptibility, and outcomes remains fragmented. We systematically reviewed published clinically significant human infections caused by filamentous basidiomycetes. Clinical, microbiological, radiological, treatment, susceptibility, and outcome data were extracted, cases from India were compared with those from the rest of the world (RoW), and logistic regression identified independent mortality predictors. Antifungal susceptibility data were summarized descriptively. We included 253 cases from 112 studies, comprising 56 from India and 197 from RoW. Respiratory or sinonasal disease without demonstrated tissue invasion predominated, although invasive and localized/non-invasive disease also occurred. Among patients with known immune status, 61.7% were immunocompetent, significantly more frequently in India. Schizophyllum commune (38.3%), Bjerkandera adusta (29.2%), and Hormographiella aspergillata (9.5%) predominated. Molecular sequencing was used for species-level identification in 62.5% of cases. Outcomes were available for 238 patients; 33 died (13.9%). Immunocompromised status (adjusted odds ratio [aOR], 7.12; 95% CI, 1.44-35.19) and histologic invasion (aOR, 3.19; 95% CI, 1.10-9.28) independently predicted mortality, whereas surgery was associated with lower observed mortality (aOR, 0.07; 95% CI, 0.01-0.66), although causality cannot be inferred. Amphotericin B and mold-active triazoles, particularly voriconazole, posaconazole, and isavuconazole, showed the most favorable in vitro activity, whereas fluconazole, 5-flucytosine, and echinocandins showed limited activity. Filamentous basidiomycetes cause a broad spectrum of disease in immunocompetent and immunocompromised hosts. Molecular identification is essential for accurate diagnosis, while immune status and tissue invasion are important independent determinants of adverse clinical outcomes. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Vinayan, Sneha AU - Vinayan S AD - Department of Neuromicrobiology, National Institute of Mental Health and Neuro Sciences, Bengaluru, Karnataka, India. FAU - S, Nagarathna AU - S N AD - Department of Neuromicrobiology, National Institute of Mental Health and Neuro Sciences, Bengaluru, Karnataka, India. FAU - Samaddar, Arghadip AU - Samaddar A AUID- ORCID: 0000-0002-4444-4274 AD - Department of Neuromicrobiology, National Institute of Mental Health and Neuro Sciences, Bengaluru, Karnataka, India. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Med Mycol JT - Medical mycology JID - 9815835 SB - IM OAB - This systematic review synthesizes 253 global cases of human filamentous basidiomycete infections, highlighting their clinical spectrum, diagnostic challenges, antifungal susceptibility, and mortality predictors. It emphasizes the need for improved molecular diagnosis and standardized management. OABL- eng OTO - NOTNLM OT - Bjerkandera adusta OT - Ceriporia lacerata OT - Hormographiella aspergillata OT - Schizophyllum commune OT - amphotericin B OT - filamentous basidiomycetes OT - internal transcribed spacer OT - voriconazole EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 11:23 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 11:23 [entrez] AID - 9003816 [pii] AID - 10.1093/mmy/myag115 [doi] PST - aheadofprint SO - Med Mycol. 2026 Oct 28:myag115. doi: 10.1093/mmy/myag115. PMID- 42949669 OWN - NLM STAT- Publisher LR - 20261028 IS - 2051-6355 (Electronic) IS - 2051-6347 (Linking) DP - 2026 Oct 28 TI - From goldene to noblene: exhaustive enumeration of the ordered Au-Ag-Cu monolayer alloys. LID - 10.1039/d6mh01971d [doi] AB - Two-dimensional metals became a laboratory reality with the isolation of goldene, a gold sheet one atom thick released by chemical exfoliation, which raises the question of what alloying can achieve on the same close-packed lattice. Here we combine an exhaustive enumeration of derivative superstructures with density functional theory to map the ordered Au-Ag-Cu monolayer alloys, relaxing every symmetry-inequivalent arrangement up to four atoms per cell and extending the set with larger cells selected by a fitted model. We find that composition sets the scale of the mixing energy while the arrangement of the atoms adds a spread of 13 meV per atom within a fixed cell stoichiometry, a spread that at one stoichiometry exceeds 50 meV per atom between two arrangements of the same three atoms and at two stoichiometries decides the sign. The energetics is carried by a competition between Au-Cu contacts, which bind, and Ag-Cu contacts, which do not, and bulk enthalpies computed under the same protocol show that all three unlike-pair interactions strengthen per bond in the monolayer, with Au-Cu the outlier at more than twice its bulk value. A pair model completed by an elastic size-mismatch term reproduces the computed energies with a mean absolute error of 5.5 meV per atom and returns a composition landscape in which no balanced ternary composition is resolved as reaching the convex hull. One equimolar ordering, which we name noblene, is the only structure in which every atom is surrounded exclusively by unlike species, and it is dynamically stable and metallic, and carries the highest Poisson's ratio of the four, 0.507 against 0.448 for the highest of the pure monolayers. Since noblene sits on the lattice that goldene already realizes, the route that produced goldene is a plausible starting point for its synthesis. FAU - Pereira Junior, Marcelo Lopes AU - Pereira Junior ML AUID- ORCID: 0000-0001-9058-510X AD - Department of Electrical Engineering, College of Technology, University of Brasilia, Federal District, 70910-900, Brasilia, Brazil. marcelo.lopes@unb.br. AD - NanoEngineering Laboratory, College of Technology, University of Brasilia, Federal District, 70910-900, Brasilia, Brazil. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Mater Horiz JT - Materials horizons JID - 101623537 SB - IM EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 11:23 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 11:23 [entrez] AID - 10.1039/d6mh01971d [doi] PST - aheadofprint SO - Mater Horiz. 2026 Oct 28. doi: 10.1039/d6mh01971d. PMID- 42949663 OWN - NLM STAT- Publisher LR - 20261028 IS - 1460-4744 (Electronic) IS - 0306-0012 (Linking) DP - 2026 Oct 28 TI - Design guidelines for body-conformable aqueous zinc-based batteries. LID - 10.1039/d6cs00347h [doi] AB - Advanced wearable medical devices represent a key direction for transforming traditional healthcare systems, where aqueous zinc-based batteries (AZBs) with inherent electrochemical safety serve as promising flexible energy storage solutions. However, conventional battery designs remain too rigid for conformal integration. This review presents a system-level design guideline for body-conformable AZBs, linking application-specific mechanical principles with scalable fabrication, material selection, and parameter optimization. We compare physical models that relate filler content, orientation, and contact topology to effective conductivity, clarifying how deformation-induced changes in conductive-network connectivity govern the electromechanical performance of stretchable electrodes. Subsequently, we introduce innovative structural strategies that impart device-level stretchability without relying on the intrinsic stretchability of materials and analyze quantitative relationships between electrode geometric parameters and mechanical properties. Finally, we highlight emerging advanced cathode chemistries, including sulfur cathodes, four-electron iodine redox chemistry, and electrolytic MnO(2) cathodes that have the potential to overcome the energy-density bottleneck, as well as polymer cathodes and air cathodes with high potential for body-conformable applications. This review offers systematic design principles and accelerates the translation of AZBs toward body-conformable healthcare electronics. FAU - Li, Xiaofeng AU - Li X AD - State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, P. R. China. cyj.strive@csu.edu.cn. AD - School of Materials Science and Engineering, Hunan Provincial Key Laboratory of Electronic Packaging and Advanced Functional Materials, Central South University, Changsha 410083, P. R. China. FAU - Chen, Yuejiao AU - Chen Y AD - State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, P. R. China. cyj.strive@csu.edu.cn. FAU - Zhou, Jiang AU - Zhou J AUID- ORCID: 0000-0003-0858-4533 AD - State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, P. R. China. cyj.strive@csu.edu.cn. AD - School of Materials Science and Engineering, Hunan Provincial Key Laboratory of Electronic Packaging and Advanced Functional Materials, Central South University, Changsha 410083, P. R. China. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - Chem Soc Rev JT - Chemical Society reviews JID - 0335405 SB - IM EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 11:13 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 11:13 [entrez] AID - 10.1039/d6cs00347h [doi] PST - aheadofprint SO - Chem Soc Rev. 2026 Oct 28. doi: 10.1039/d6cs00347h. PMID- 42949657 OWN - NLM STAT- Publisher LR - 20261028 IS - 1463-9084 (Electronic) IS - 1463-9076 (Linking) DP - 2026 Oct 28 TI - Structure and interaction of mixed ionic liquids with lithium using first-principles calculations. LID - 10.1039/d6cp02281b [doi] AB - This study employs first-principles and ab initio molecular dynamics (AIMD) calculations to investigate the interactions between mixed ionic-liquid systems (ILs) and lithium-metal surfaces. The systems contain two cations, [C(4)mpyr](+) and [C(4)mpip](+), and five anions: [FSI](-), [TFSI](-), [FTFSI](-), [MCTFSI](-), and [OTF](-). They were arranged in checkerboard configurations on Li(100), and their interfacial interactions were analyzed using the independent gradient model (IGM), electron density difference (EDD), and charge analysis. Replacing [C(4)mpyr](+) with [C(4)mpip](+) produces stronger adsorption in the FSI-, FTFSI-, and MCTFSI-containing systems, but weaker adsorption in the TFSI- and OTF-containing systems. Cation and anion mixing also reduces hydrogen bonding in selected systems, suggesting a potentially favorable effect on electrolyte transport; however, the present vacuum-monolayer model cannot directly quantify bulk viscosity or ionic conductivity. AIMD simulations further indicate that adding [FSI](-) leads to an earlier onset of [MCTFSI](-) decomposition within the simulated trajectories. These findings provide microscopic insight into the initial interactions of mixed ionic liquids with lithium. FAU - Dai, Fu AU - Dai F AD - National Engineering Research Center of Vacuum Metallurgy, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. szzhang@kust.edu.cn. AD - Engineering Laboratory for Advanced Battery and Materials of Yunnan Province, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. FAU - Zeng, Yixing AU - Zeng Y AD - National Engineering Research Center of Vacuum Metallurgy, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. szzhang@kust.edu.cn. AD - Engineering Laboratory for Advanced Battery and Materials of Yunnan Province, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. FAU - Zhang, Shaoze AU - Zhang S AUID- ORCID: 0000-0001-9387-2462 AD - National Engineering Research Center of Vacuum Metallurgy, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. szzhang@kust.edu.cn. AD - Engineering Laboratory for Advanced Battery and Materials of Yunnan Province, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. FAU - Yao, Yaochun AU - Yao Y AUID- ORCID: 0000-0001-6837-1789 AD - National Engineering Research Center of Vacuum Metallurgy, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. szzhang@kust.edu.cn. AD - Engineering Laboratory for Advanced Battery and Materials of Yunnan Province, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. FAU - Luo, Dengyang AU - Luo D AD - National Engineering Research Center of Vacuum Metallurgy, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. szzhang@kust.edu.cn. AD - Engineering Laboratory for Advanced Battery and Materials of Yunnan Province, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. AD - Southwest United Graduate School, Kunming 650091, Yunnan Province, China. FAU - Yang, Rui AU - Yang R AD - National Engineering Research Center of Vacuum Metallurgy, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. szzhang@kust.edu.cn. AD - Engineering Laboratory for Advanced Battery and Materials of Yunnan Province, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. FAU - Zhou, Fushuang AU - Zhou F AD - National Engineering Research Center of Vacuum Metallurgy, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. szzhang@kust.edu.cn. AD - Engineering Laboratory for Advanced Battery and Materials of Yunnan Province, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. FAU - Zhang, Keyu AU - Zhang K AUID- ORCID: 0000-0001-5296-9735 AD - National Engineering Research Center of Vacuum Metallurgy, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. szzhang@kust.edu.cn. AD - Engineering Laboratory for Advanced Battery and Materials of Yunnan Province, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. FAU - Li, Yin AU - Li Y AUID- ORCID: 0009-0000-0120-840X AD - National Engineering Research Center of Vacuum Metallurgy, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. szzhang@kust.edu.cn. AD - Engineering Laboratory for Advanced Battery and Materials of Yunnan Province, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. FAU - Hu, Junxian AU - Hu J AUID- ORCID: 0000-0003-0139-207X AD - National Engineering Research Center of Vacuum Metallurgy, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. szzhang@kust.edu.cn. AD - Engineering Laboratory for Advanced Battery and Materials of Yunnan Province, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. FAU - Yang, Bin AU - Yang B AD - National Engineering Research Center of Vacuum Metallurgy, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. szzhang@kust.edu.cn. AD - Engineering Laboratory for Advanced Battery and Materials of Yunnan Province, School of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming 650093, Yunnan Province, China. FAU - He, Yan AU - He Y AD - Jiangxi Province Key Laboratory of Functional Organic Polymers, School of Chemistry and Materials Science, East China University of Technology, Nanchang 330013, China. FAU - Liu, Honglai AU - Liu H AD - Key Laboratory for Advanced Materials and School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Phys Chem Chem Phys JT - Physical chemistry chemical physics : PCCP JID - 100888160 SB - IM EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 CRDT- 2026/10/28 11:13 PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 11:13 [entrez] AID - 10.1039/d6cp02281b [doi] PST - aheadofprint SO - Phys Chem Chem Phys. 2026 Oct 28. doi: 10.1039/d6cp02281b. PMID- 42949655 OWN - NLM STAT- Publisher LR - 20261028 IS - 2198-3844 (Electronic) IS - 2198-3844 (Linking) DP - 2026 Oct 28 TI - Noninvasive Detection and Subtyping of Esophageal Cancer by Circulating Cell-Free mtDNA Fragmentomics: A Multi-Center Validation Study. LID - e78377 [pii] LID - 10.1002/advs.78377 [doi] AB - Esophageal cancer (EC) is highly lethal due to late diagnosis, and accurate non-invasive tools for early detection and subtyping remain lacking. Circulating cell-free mitochondrial DNA (ccf-mtDNA) fragmentomics offers a promising biomarker source. In this large multi-center study enrolling 1,260 participants spanning healthy control (HC), benign esophageal diseases (BED), precancerous lesions and EC patients, we developed two machine learning models based on plasma ccf-mtDNA fragmentomic profiles: an EC detection (ED) model and a pathological subtyping prediction (PSP) model. The ED model achieved an AUC of 0.9706 (EC vs. non-EC) in the training cohort, with high performance in internal (0.9762), external (0.9748), and Chinese minority (0.9668, Uyghur and Kazakh) validation cohorts. At the optimal cut-off, it yielded 92.16% sensitivity for stage I EC in the combined validation cohort and correctly identified 93.10% of early EC cases missed by preoperative biopsy. It also discriminated high-grade intraepithelial neoplasia from non-EC with an AUC of 0.9597. The PSP model distinguished ESCC from EAC with 89.71% accuracy, improving to 90.74% after integrating key clinical parameters. In summary, ccf-mtDNA fragmentomics provides a robust, non-invasive platform for early EC detection, precancerous risk stratification, and histological subtyping, demonstrating strong potential to transform clinical management. CI - (c) 2026 The Author(s). Advanced Science published by Wiley-VCH GmbH. FAU - Liu, Yue AU - Liu Y AUID- ORCID: 0009-0005-2831-5199 AD - State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and Department of Physiology and Pathophysiology, Fourth Military Medical University, Xi'an, China. FAU - Yang, Wenying AU - Yang W AD - College of Medical Technology, Shaanxi University of Chinese Medicine, Xianyang, China. FAU - Li, Yu AU - Li Y AD - State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and Department of Physiology and Pathophysiology, Fourth Military Medical University, Xi'an, China. FAU - Wang, Siyuan AU - Wang S AD - State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and Department of Physiology and Pathophysiology, Fourth Military Medical University, Xi'an, China. FAU - Li, Xiang AU - Li X AD - Department of Clinical Laboratory, The First Affiliated Hospital of Air Force Medical University, Xi'an, China. FAU - Peng, Fan AU - Peng F AD - State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and Department of Physiology and Pathophysiology, Fourth Military Medical University, Xi'an, China. FAU - Dang, Miao AU - Dang M AUID- ORCID: 0009-0003-4467-4269 AD - State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and Department of Physiology and Pathophysiology, Fourth Military Medical University, Xi'an, China. FAU - Zhang, Huanqin AU - Zhang H AD - State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and Department of Physiology and Pathophysiology, Fourth Military Medical University, Xi'an, China. FAU - Jiao, Huanmin AU - Jiao H AD - State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and Department of Physiology and Pathophysiology, Fourth Military Medical University, Xi'an, China. FAU - Yang, Yuqing AU - Yang Y AD - State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and Department of Physiology and Pathophysiology, Fourth Military Medical University, Xi'an, China. FAU - Su, Zhaoquan AU - Su Z AUID- ORCID: 0009-0009-3847-1178 AD - Clinical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical University, Urumqi, China. FAU - Ma, Xiumin AU - Ma X AUID- ORCID: 0000-0001-8011-7513 AD - Clinical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical University, Urumqi, China. FAU - Gong, Zhiyun AU - Gong Z AD - Department of Clinical Laboratory, Fudan University Shanghai Cancer Center, Shanghai, China. FAU - Lu, Renquan AU - Lu R AUID- ORCID: 0000-0003-3291-5742 AD - Department of Clinical Laboratory, Fudan University Shanghai Cancer Center, Shanghai, China. FAU - Xing, Jinliang AU - Xing J AUID- ORCID: 0000-0003-2532-7029 AD - State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and Department of Physiology and Pathophysiology, Fourth Military Medical University, Xi'an, China. FAU - Liu, Yang AU - Liu Y AUID- ORCID: 0009-0007-3811-6163 AD - State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers and Department of Clinical Diagnosis, Tangdu Hospital, Fourth Military Medical University, Xi'an, China. LA - eng GR - 82330073/National Natural Science Foundation of China/ GR - 82402731/National Natural Science Foundation of China/ GR - 2024SF-ZDCYL-02-02/Science & Technology Co-ordination and Innovation Project of Shaanxi Province, China/ GR - 2023LC2324/Clinical Research Project of Air Force Medical University/ GR - 2023LC2315/Clinical Research Project of Air Force Medical University/ GR - 2023ZD0510900/Noncommunicable Chronic Diseases-National Science and Technology Major Project/ GR - SQ2023AAA031957/National Science and Technology Major Project Youth Talent Cultivation Program/ PT - Journal Article DEP - 20261028 PL - Germany TA - Adv Sci (Weinh) JT - Advanced science (Weinheim, Baden-Wurttemberg, Germany) JID - 101664569 SB - IM PMC - PMC13715668 OTO - NOTNLM OT - circulating cell-free mitochondrial DNA OT - early detection OT - esophageal cancer OT - fragmentomics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:31 PMCR- 2026/10/28 CRDT- 2026/10/28 11:13 PHST- 2026/09/15 00:00 [revised] PHST- 2026/04/15 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/28 17:31 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 11:13 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e78377 [pii] AID - ADVS78377 [pii] AID - 10.1002/advs.78377 [doi] PST - aheadofprint SO - Adv Sci (Weinh). 2026 Oct 28:e78377. doi: 10.1002/advs.78377. PMID- 42949645 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1754-8411 (Electronic) IS - 1754-8403 (Linking) VI - 19 IP - 10 DP - 2026 Oct 1 TI - Mouse models of gastric preneoplasia and cancer, with a focus on pathology. LID - dmm052902 [pii] LID - 10.1242/dmm.052902 [doi] AB - Gastric cancer is the fifth most diagnosed cancer and the fifth leading cause of cancer deaths worldwide, with limited options of targeted therapy available. Thus, a better understanding of key molecular events that contribute to gastric tumorigenesis would facilitate the development of novel therapies to prevent or treat gastric cancer. Extensive efforts have been made towards generating murine models that recapitulate the complex process of gastric cancer pathogenesis. There have been some challenges, however, in identifying and interpreting the results obtained by using these models. Namely, hyperplasias have often been misinterpreted as invasive cancer, and identifying cancer cells of origin has been complicated by nonspecific lineage-tracing studies. In this Review, we summarize the current models of gastric preneoplasia and cancer and discuss their limitations, especially with regard to correlating mouse and human pathology. CI - (c) 2026. Published by The Company of Biologists. FAU - Ho, Raymond AU - Ho R AUID- ORCID: 0000-0002-6043-4287 AD - Department of Molecular and Precision Medicine, Penn State College of Medicine, Hershey, PA 17033, USA. AD - Medical Scientist Training Program, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Zeng, Yongji AU - Zeng Y AD - Department of Molecular and Precision Medicine, Penn State College of Medicine, Hershey, PA 17033, USA. FAU - Mills, Jason C AU - Mills JC AUID- ORCID: 0000-0002-0402-4662 AD - Department of Molecular and Precision Medicine, Penn State College of Medicine, Hershey, PA 17033, USA. LA - eng GR - R01DK094989/DK/NIDDK NIH HHS/United States GR - R01DK134531/DK/NIDDK NIH HHS/United States GR - R01DK105129/DK/NIDDK NIH HHS/United States GR - R01CA239645/CA/NCI NIH HHS/United States PT - Journal Article PT - Review DEP - 20261028 PL - England TA - Dis Model Mech JT - Disease models & mechanisms JID - 101483332 SB - IM MH - Animals MH - *Stomach Neoplasms/pathology MH - Humans MH - Disease Models, Animal MH - *Precancerous Conditions/pathology MH - Mice OTO - NOTNLM OT - Gastric cancer OT - Intestinal metaplasia OT - Mouse models OT - Paligenosis OT - Pseudoinvasion OT - SPEM COIS- Competing interests The authors declare no competing or financial interests. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 CRDT- 2026/10/28 11:03 PHST- 2026/02/28 00:00 [received] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 11:03 [entrez] AID - 373314 [pii] AID - 10.1242/dmm.052902 [doi] PST - ppublish SO - Dis Model Mech. 2026 Oct 1;19(10):dmm052902. doi: 10.1242/dmm.052902. Epub 2026 Oct 28. PMID- 42949643 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2001-1326 (Electronic) IS - 2001-1326 (Linking) VI - 16 IP - 11 DP - 2026 Nov TI - Toward a digital urinary biopsy for early risk stratification in diabetic kidney disease: Anchoring shed cells, vesicles, and cell-free DNA to spatial kidney cell states. LID - e70866 [pii] LID - 10.1002/ctm2.70866 [doi] AB - BACKGROUND: Diabetic kidney disease (DKD) is the leading cause of end-stage kidney disease worldwide. However, albuminuria and estimated glomerular filtration rate (eGFR), which define and stage DKD, report established injury and do not resolve the cellular heterogeneity underlying variable progression. Urine, the only biofluid to traverse every nephron segment, carries shed cells, extracellular vesicles (EVs) and cell-free DNA (cfDNA), each retaining information about its cell of origin. MAIN BODY: This review develops the concept of a digital urinary biopsy, in which the three compartments are read together against spatial single-cell kidney atlases to infer non-invasively which cell states are active and whether niche-associated programs are engaged, an approach we term atlas-anchored molecular profiling. For each compartment, we tabulate study population, sample size, processing, assay, performance and validation status. Because shed-cell RNA, selectively sorted vesicle cargo and cfDNA methylation differ in origin, measurement scale and bias, each requires its own reference matrix, sampling function and background terms. All three carry renal information in feasibility studies, yet none is a validated clinical assay in DKD. Moreover, a cfDNA methylation reference at the required resolution is lacking, and no named computational method has been tested on urine. CONCLUSION: We therefore present the framework as a structured research hypothesis and define the required validation, from analytical and clinical validity to prospective demonstration of utility, with non-albuminuric DKD, which albuminuria-based screening misses, as the index use case. It offers a testable route to cell-resolved, non-invasive staging of DKD rather than a demonstrated capability. CI - (c) 2026 The Author(s). Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics. FAU - Wu, Juan AU - Wu J AD - Department of Geriatrics, Xiangya Hospital, Central South University, Changsha, Hunan, China. AD - National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, China. FAU - Sheng, Ping AU - Sheng P AD - Department of General Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, China. FAU - Lu, Ying AU - Lu Y AD - Department of General Medicine, Xiangya Hospital, Central South University, Changsha, Hunan, China. FAU - Chen, Ke AU - Chen K AUID- ORCID: 0009-0007-1557-7282 AD - Department of Geriatrics, Xiangya Hospital, Central South University, Changsha, Hunan, China. AD - National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, Changsha, Hunan, China. LA - eng PT - Journal Article PT - Review PL - United States TA - Clin Transl Med JT - Clinical and translational medicine JID - 101597971 RN - 0 (Cell-Free Nucleic Acids) SB - IM MH - Humans MH - *Diabetic Nephropathies/diagnosis/urine/physiopathology/pathology MH - *Cell-Free Nucleic Acids/analysis/urine MH - *Extracellular Vesicles MH - Risk Assessment/methods MH - Biopsy/methods MH - *Kidney/pathology/physiopathology PMC - PMC13715840 OTO - NOTNLM OT - cell-free DNA methylation OT - diabetic kidney disease OT - extracellular vesicles OT - single-cell transcriptomics OT - urinary liquid biopsy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 11:03 PHST- 2026/09/24 00:00 [revised] PHST- 2026/07/30 00:00 [received] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 11:03 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e70866 [pii] AID - CTM270866 [pii] AID - 10.1002/ctm2.70866 [doi] PST - ppublish SO - Clin Transl Med. 2026 Nov;16(11):e70866. doi: 10.1002/ctm2.70866. PMID- 42949639 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1929-0748 (Electronic) IS - 1929-0748 (Linking) VI - 15 DP - 2026 Oct 28 TI - Efficacy and Mechanism of Transcutaneous Auricular Vagus Nerve Stimulation in Patients With Poststroke Dysphagia: Protocol for a Randomized, Sham-Controlled Clinical Trial. PG - e95182 LID - 10.2196/95182 [doi] LID - e95182 AB - BACKGROUND: Dysphagia is a common complication following stroke. While current management strategies for poststroke dysphagia (PSD) range from nutritional support to neurostimulation techniques, an optimal treatment regimen remains elusive. As a promising noninvasive neuromodulation approach, transcutaneous auricular vagus nerve stimulation (taVNS) requires further validation in rigorously designed trials to establish robust evidence of its efficacy and mechanisms of action. OBJECTIVE: This study aims to validate the efficacy of taVNS in treating PSD and to elucidate its underlying mechanisms, thereby supporting the development of a novel, noninvasive therapeutic strategy for improving swallowing function in stroke survivors. METHODS: This study will use a prospective, randomized, double-blind, controlled trial design. A total of 40 patients with PSD will be enrolled and randomly allocated to either a taVNS group or a sham taVNS group. Both groups will receive conventional swallowing rehabilitation therapy as the baseline intervention. The taVNS group will receive bilateral electrical stimulation at the cymba conchae and cavum conchae of the auricle. These sites correspond to the "Heart" and "Kidney" auricular acupoints, selected in accordance with traditional Chinese medicine theory. In contrast, the sham taVNS group will receive stimulation at regions of the auricle not innervated by the vagus nerve. The primary outcome measure will be the Penetration-Aspiration Scale score. Secondary outcomes will include the Yale Pharyngeal Residue Severity Rating Scale, hyoid bone displacement and hyoid-thyroid approximation distance assessed via B-mode ultrasonography, and motor-evoked potentials of the suprahyoid muscles elicited by transcranial magnetic stimulation. All outcome measures will be evaluated at baseline and after the 2-week intervention period. RESULTS: This paper describes the protocol for a study evaluating the efficacy and mechanisms of taVNS in patients with PSD. The study was funded in January 2025. Participant recruitment began in March 2025 and is projected to conclude by October 2026. Data collection began in March 2025 and is projected to be completed by October 2026. Primary study results are expected to be submitted for publication in winter 2026. CONCLUSIONS: This study aims to validate the efficacy of taVNS in treating PSD and to elucidate its underlying mechanisms, thereby supporting the development of a novel, noninvasive therapeutic strategy for improving swallowing function in stroke survivors. CI - (c) Luming Gao, Jin Li, Miao Xie, Canyi Xu, Changying Zhang, Weichuan Kuang, Xi Wen, Xiaoyin Wang, Yue Liu, Yu Wang, Tong Liu. Originally published in JMIR Research Protocols (https://www.researchprotocols.org). FAU - Gao, Luming AU - Gao L AUID- ORCID: 0009-0002-8783-8553 AD - The Fifth College of Clinical Medicine, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China. FAU - Li, Jin AU - Li J AUID- ORCID: 0009-0004-0228-1210 AD - The Fifth College of Clinical Medicine, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China. AD - Guangdong Provincial Engineering Technology Research Institute of Traditional Chinese Medicine, Guangzhou, Guangdong, China. AD - Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine, Guangzhou, Guangdong, China. AD - Department of Acupuncture and Rehabilitation, Guangdong Provincial Second Hospital of Traditional Chinese Medicine, 60 Hengfu Road, Yuexiu District, Guangzhou, Guangdong, China, 86 18319673139. FAU - Xie, Miao AU - Xie M AUID- ORCID: 0009-0009-0503-6372 AD - The Fifth College of Clinical Medicine, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China. FAU - Xu, Canyi AU - Xu C AUID- ORCID: 0009-0002-3325-2450 AD - The Fifth College of Clinical Medicine, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China. FAU - Zhang, Changying AU - Zhang C AUID- ORCID: 0009-0000-7988-8783 AD - The Fifth College of Clinical Medicine, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China. FAU - Kuang, Weichuan AU - Kuang W AUID- ORCID: 0009-0001-1970-7431 AD - Department of Acupuncture and Rehabilitation, Guangdong Provincial Second Hospital of Traditional Chinese Medicine, 60 Hengfu Road, Yuexiu District, Guangzhou, Guangdong, China, 86 18319673139. FAU - Wen, Xi AU - Wen X AUID- ORCID: 0000-0002-1222-260X AD - Department of Acupuncture and Rehabilitation, Guangdong Provincial Second Hospital of Traditional Chinese Medicine, 60 Hengfu Road, Yuexiu District, Guangzhou, Guangdong, China, 86 18319673139. FAU - Wang, Xiaoyin AU - Wang X AUID- ORCID: 0009-0005-5852-4071 AD - Department of Acupuncture and Rehabilitation, Guangdong Provincial Second Hospital of Traditional Chinese Medicine, 60 Hengfu Road, Yuexiu District, Guangzhou, Guangdong, China, 86 18319673139. FAU - Liu, Yue AU - Liu Y AUID- ORCID: 0009-0006-7345-6270 AD - Department of Acupuncture and Rehabilitation, Guangdong Provincial Second Hospital of Traditional Chinese Medicine, 60 Hengfu Road, Yuexiu District, Guangzhou, Guangdong, China, 86 18319673139. FAU - Wang, Yu AU - Wang Y AUID- ORCID: 0000-0001-5070-0038 AD - Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing, Beijing, China. FAU - Liu, Tong AU - Liu T AUID- ORCID: 0000-0002-5185-2188 AD - The Fifth College of Clinical Medicine, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China. AD - Guangdong Provincial Key Laboratory of Research and Development in Traditional Chinese Medicine, Guangzhou, Guangdong, China. AD - Department of Acupuncture and Rehabilitation, Guangdong Provincial Second Hospital of Traditional Chinese Medicine, 60 Hengfu Road, Yuexiu District, Guangzhou, Guangdong, China, 86 18319673139. LA - eng PT - Clinical Trial Protocol PT - Journal Article DEP - 20261028 PL - Canada TA - JMIR Res Protoc JT - JMIR research protocols JID - 101599504 SB - IM MH - Humans MH - *Deglutition Disorders/therapy/etiology MH - *Stroke/complications MH - Double-Blind Method MH - Randomized Controlled Trials as Topic MH - *Vagus Nerve Stimulation/methods MH - Prospective Studies MH - *Transcutaneous Electric Nerve Stimulation/methods MH - Female MH - Male MH - Treatment Outcome MH - Middle Aged MH - Adult MH - Aged PMC - PMC13715380 OTO - NOTNLM OT - poststroke dysphagia OT - protocol OT - randomized controlled trial OT - stroke OT - transcutaneous auricular vagus nerve stimulation COIS- Conflicts of Interest: None declared. EDAT- 2026/10/28 17:31 MHDA- 2026/10/28 17:32 PMCR- 2026/10/28 CRDT- 2026/10/28 11:03 PHST- 2026/03/17 00:00 [received] PHST- 2026/07/08 00:00 [revised] PHST- 2026/07/16 00:00 [accepted] PHST- 2026/10/28 17:32 [medline] PHST- 2026/10/28 17:31 [pubmed] PHST- 2026/10/28 11:03 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - v15i1e95182 [pii] AID - 95182 [pii] AID - 10.2196/95182 [doi] PST - epublish SO - JMIR Res Protoc. 2026 Oct 28;15:e95182. doi: 10.2196/95182. PMID- 42949634 OWN - NLM STAT- Publisher LR - 20261028 IS - 1364-5528 (Electronic) IS - 0003-2654 (Linking) DP - 2026 Oct 28 TI - Non-destructive discrimination of black gel pen inks via physics-guided band-gate attention network and hyperspectral imaging. LID - 10.1039/d6an00923a [doi] AB - The non-destructive identification of black gel pen inks and writing-to-alteration intervals is important in forensic ink analysis, particularly for legal and financial documents. This study proposes an intelligent framework integrating hyperspectral imaging (HSI) with a novel deep learning model, PG-BCANet, for objective ink characterization. Hyperspectral data were collected from black gel pen samples on standard A4 paper, representing different pen models and specified writing-to-alteration intervals. After noise suppression and baseline drift correction using Savitzky-Golay (SG) smoothing and Standard Normal Variate (SNV) normalization, three feature selection algorithms, including Principal Component Analysis (PCA), Interval Random Forest (iRF), and Successive Projections Algorithm (SPA), were comparatively evaluated. For classification, the proposed PG-BCANet, constructed on a 1-D Convolutional Neural Network (1DCNN), incorporates a Band-Gate mechanism, Efficient Channel Attention (ECA), and residual connections to optimize feature extraction from high-dimensional spectral data. PG-BCANet achieved accuracies of 96.6% for model identification and up to 89.2% for discrimination among the investigated temporal intervals. These results exceeded the corresponding KNN and SVM baselines under the adopted experimental protocol. This research provides a non-destructive approach for forensic ink analysis and supports further investigation of deep learning-enhanced HSI in forensic science. FAU - Qian, Qian AU - Qian Q AD - College of Optical and Electronic Technology, China Jiliang University, Hangzhou 310018, China. yinlu890622@163.com. FAU - Li, Yan AU - Li Y AD - College of Optical and Electronic Technology, China Jiliang University, Hangzhou 310018, China. yinlu890622@163.com. FAU - Zhang, Xinri AU - Zhang X AD - College of Optical and Electronic Technology, China Jiliang University, Hangzhou 310018, China. yinlu890622@163.com. FAU - Xie, Xinrui AU - Xie X AD - College of Optical and Electronic Technology, China Jiliang University, Hangzhou 310018, China. yinlu890622@163.com. FAU - Yin, Lu AU - Yin L AUID- ORCID: 0000-0001-8179-1569 AD - College of Optical and Electronic Technology, China Jiliang University, Hangzhou 310018, China. yinlu890622@163.com. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Analyst JT - The Analyst JID - 0372652 SB - IM EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 10:54 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 10:54 [entrez] AID - 10.1039/d6an00923a [doi] PST - aheadofprint SO - Analyst. 2026 Oct 28. doi: 10.1039/d6an00923a. PMID- 42949628 OWN - NLM STAT- Publisher LR - 20261028 IS - 1521-3773 (Electronic) IS - 1433-7851 (Linking) DP - 2026 Oct 28 TI - Molecular Basis of NPY Recognition and Ligand Selectivity at the Neuropeptide Y(5) Receptor. LID - e4104254 [pii] LID - 10.1002/anie.4104254 [doi] AB - Neuropeptide Y receptors (YRs) have been demonstrated to mediate physiological functions such as food intake, energy expenditure and mood regulation. Despite the low sequence identity exhibited by the four YR subtypes, Y(1)R, Y(2)R, Y(4)R, and Y(5)R, all of them recognize the endogenous ligand neuropeptide Y (NPY), which demonstrates their complex ligand recognition and activation mechanisms. The Y(5)R is regarded as a promising drug target for cachexia and cancer, but drug development has been hampered by the lack of structural information on ligand-Y(5)R interactions. We report the cryo-electron microscopy structure of Y(5)R in complex with NPY and the G(i1) protein, revealing unique features of the Y(5)R extracellular regions that participate in ligand recognition and illustrating the specific binding mode of the NPY C-terminus to Y(5)R. The structural information is supported by mutagenesis studies investigating ligand binding and receptor activation with the endogenous ligand NPY and the selective Y(5)R agonist sY(5)ago. Structural analysis provides valuable insights into the factors driving receptor subtype selectivity, thus offering an opportunity for drug discovery of Y(5)R. CI - (c) 2026 The Author(s). Angewandte Chemie International Edition published by Wiley-VCH GmbH. FAU - Peuker, Lisa Christin AU - Peuker LC AUID- ORCID: 0000-0002-1405-8129 AD - Institute of Biochemistry, Leipzig University, Leipzig, Germany. FAU - Wang, Hongyu AU - Wang H AUID- ORCID: 0000-0001-8003-796X AD - School of Pharmacy, Fudan University, Shanghai, China. AD - State Key Laboratory of Drug Research, State Key Laboratory of Chemical Biology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Chen, Kun AU - Chen K AD - State Key Laboratory of Drug Research, State Key Laboratory of Chemical Biology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China. AD - University of Chinese Academy of Sciences, Beijing, China. AD - Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Zhongshan, China. FAU - Lobner, Kristin AU - Lobner K AUID- ORCID: 0009-0002-0530-0064 AD - Institute of Biochemistry, Leipzig University, Leipzig, Germany. FAU - Morl, Karin AU - Morl K AUID- ORCID: 0000-0002-9399-9428 AD - Institute of Biochemistry, Leipzig University, Leipzig, Germany. FAU - Schwarz, Simon Jonas AU - Schwarz SJ AUID- ORCID: 0009-0007-2075-700X AD - Institute of Biochemistry, Leipzig University, Leipzig, Germany. FAU - Zhao, Qiang AU - Zhao Q AD - State Key Laboratory of Drug Research, State Key Laboratory of Chemical Biology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China. AD - University of Chinese Academy of Sciences, Beijing, China. AD - Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Zhongshan, China. FAU - Wu, Beili AU - Wu B AD - State Key Laboratory of Drug Research, State Key Laboratory of Chemical Biology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China. AD - University of Chinese Academy of Sciences, Beijing, China. AD - School of Pharmaceutical Science and Technology, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China. AD - Shanghai Academy of Natural Sciences (SANS), Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China. FAU - Beck-Sickinger, Annette Gabriele AU - Beck-Sickinger AG AUID- ORCID: 0000-0003-4560-8020 AD - Institute of Biochemistry, Leipzig University, Leipzig, Germany. LA - eng GR - 421152132/Deutsche Forschungsgemeinschaft/ GR - 468939089/Deutsche Forschungsgemeinschaft/ GR - 268/552-1 FUGG/Deutsche Forschungsgemeinschaft/ GR - 2022YFA1302900/National Key Research and Development Program of China/ GR - 32161133011/National Science Foundation of China/ GR - XDB0830000/CAS Strategic Priority Research Programs/ GR - XDB1060000/CAS Strategic Priority Research Programs/ PT - Journal Article DEP - 20261028 PL - Germany TA - Angew Chem Int Ed Engl JT - Angewandte Chemie (International ed. in English) JID - 0370543 SB - IM OTO - NOTNLM OT - ligand binding mode OT - receptors OT - structure-activity relationships EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 10:53 PHST- 2026/09/09 00:00 [revised] PHST- 2026/07/23 00:00 [received] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 10:53 [entrez] AID - e4104254 [pii] AID - 10.1002/anie.4104254 [doi] PST - aheadofprint SO - Angew Chem Int Ed Engl. 2026 Oct 28:e4104254. doi: 10.1002/anie.4104254. PMID- 42949622 OWN - NLM STAT- Publisher LR - 20261028 IS - 1521-3773 (Electronic) IS - 1433-7851 (Linking) DP - 2026 Oct 28 TI - Bromide-Activated Four-Electron Iodine Chemistry Enabled by a Pyridinium-Integrated Hydrogel Electrolyte. LID - e3951000 [pii] LID - 10.1002/anie.3951000 [doi] AB - Aqueous four-electron zinc-iodine (Zn-I(2)) batteries offer high capacity and operating voltage, yet most reported systems rely on Cl(-) to activate the I(0)/I(+) conversion, generating hydrolysis-prone ICl(2) (-) intermediates that cause rapid capacity decay. However, how inner I-X bond polarization (X = F, Cl, and Br) governs the hydrolytic stability of interhalogen intermediates remains unclear. Here, we conduct both experimental and computational analyses, revealing Br(-) as the most effective halide. Compared with F(-) and Cl(-), Br(-) forms less-polarized and more hydrolytically stable IBr(2) (-) intermediates, markedly improving high-voltage iodine reversibility. Building on this Br(-)-mediated stabilization, we further design a multifunctional PNA-PyBr hydrogel electrolyte by incorporating pyridine hydrobromide into a sulfonate-rich poly(sodium p-styrenesulfonate-co-acrylamide) network. Pyridinium cations immobilize IBr(2) (-) as water-insoluble Py(+)IBr(2) (-) complexes, protecting I(+) species from hydrolysis, while sulfonate groups regulate the aqueous hydrogen-bond network and establish Zn(2+)-selective transport pathways. The resulting cells deliver a near-theoretical capacity of 422.6 mAh g(-1) and a record lifespan of over 20 000 cycles at 20 C. Moreover, a 500 mAh pouch cell with an areal capacity of 3.5 mAh cm(-2) sustains 600 cycles with negligible capacity decay, demonstrating the practical potential of Br(-)-activated four-electron Zn-I(2) chemistry. CI - (c) 2026 The Author(s). Angewandte Chemie International Edition published by Wiley-VCH GmbH. FAU - Ma, Rujiao AU - Ma R AUID- ORCID: 0000-0003-4437-1367 AD - School of Chemical Engineering, Adelaide University, Adelaide, Australia. FAU - Zhang, Shao-Jian AU - Zhang SJ AUID- ORCID: 0000-0001-7738-0425 AD - School of Chemical Engineering, Adelaide University, Adelaide, Australia. FAU - Zhao, Xun AU - Zhao X AUID- ORCID: 0000-0002-3711-4568 AD - School of Chemical Engineering, Adelaide University, Adelaide, Australia. FAU - Hu, Yiyang AU - Hu Y AUID- ORCID: 0000-0002-8758-8809 AD - School of Chemical Engineering, Adelaide University, Adelaide, Australia. FAU - Wu, Han AU - Wu H AUID- ORCID: 0000-0002-5281-7325 AD - School of Chemical Engineering, Adelaide University, Adelaide, Australia. FAU - Hao, Junnan AU - Hao J AUID- ORCID: 0000-0002-5777-7844 AD - School of Chemical Engineering, Adelaide University, Adelaide, Australia. LA - eng GR - IL230100039/Australian Research Council/ GR - DP220102596/Australian Research Council/ GR - CE230100032/Australian Research Council/ GR - Shi-Zhang Qiao, Chair of Nanotechnology at the School of Chemical Engineering/ GR - Adelaide University/ PT - Journal Article DEP - 20261028 PL - Germany TA - Angew Chem Int Ed Engl JT - Angewandte Chemie (International ed. in English) JID - 0370543 SB - IM OTO - NOTNLM OT - Br-triggered OT - Zn reversibility OT - four-electron reaction OT - hydrogel OT - shuttle-free EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 10:53 PHST- 2026/10/07 00:00 [revised] PHST- 2026/08/27 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 10:53 [entrez] AID - e3951000 [pii] AID - 10.1002/anie.3951000 [doi] PST - aheadofprint SO - Angew Chem Int Ed Engl. 2026 Oct 28:e3951000. doi: 10.1002/anie.3951000. PMID- 42949619 OWN - NLM STAT- Publisher LR - 20261028 IS - 1950-6945 (Electronic) IS - 1294-9361 (Linking) DP - 2026 Oct 28 TI - Comparative efficacy of first-line antiseizure monotherapy in pediatric focal epilepsy: An umbrella review of systematic reviews and meta-analyses. LID - 10.1002/epd2.70443 [doi] AB - INTRODUCTION: First-line antiseizure medication (ASM) monotherapy remains the recommended initial treatment for pediatric focal epilepsy. Although several ASMs are available, uncertainty persists regarding their comparative efficacy, safety, and tolerability, particularly in children. This umbrella review aimed to synthesize and map the available evidence from systematic reviews and meta-analyses evaluating first-line ASM monotherapies for focal epilepsy, with a focus on informing pediatric treatment decisions. METHODS: This umbrella review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidance. Systematic reviews and meta-analyses evaluating first-line ASM monotherapy for focal epilepsy were identified through comprehensive database searches. Data on study characteristics, patient populations, ASM comparisons, epilepsy subtypes, follow-up duration, efficacy, safety, and tolerability outcomes were extracted and synthesized descriptively. Evidence mapping was performed to summarize the findings, and potential overlap of primary studies across the included reviews was considered. Where available, outcome-specific certainty-of-evidence ratings reported by the source reviews using GRADE or CINeMA were also extracted and summarized. RESULTS: Sixteen systematic reviews and meta-analyses were included, comprising randomized controlled trials and individual participant data analyses from pediatric and mixed-age populations. Frequently evaluated ASMs included carbamazepine, lamotrigine, levetiracetam, oxcarbazepine, valproate, phenytoin, and several newer-generation agents. Across the included reviews, no single first-line ASM showed consistent superiority for seizure control outcomes. Selected comparisons suggested tolerability advantages for lamotrigine, levetiracetam, and some newer-generation ASMs; however, these findings were not uniform across outcomes or reviews. Several comparative estimates were imprecise, with confidence intervals encompassing potentially clinically meaningful effects, and substantial heterogeneity was present across epilepsy subtype definitions, patient populations, follow-up durations, and outcome reporting. The frequent inclusion of mixed-age populations further limited the strength and direct applicability of pediatric-specific conclusions. CONCLUSIONS: Current umbrella-level evidence does not establish clear superiority of one first-line ASM over another for seizure control in pediatric focal epilepsy. Importantly, the absence of statistically significant differences should not be interpreted as evidence of therapeutic equivalence, because several estimates remain imprecise and clinically meaningful differences cannot be excluded. Interpretation is further limited by the scarcity of pediatric-specific comparative data, the frequent inclusion of mixed-age populations, and methodological and outcome heterogeneity across reviews. Safety and tolerability should therefore be considered alongside efficacy when individualizing treatment selection. Well-designed pediatric-specific comparative studies using standardized outcome measures are needed to strengthen evidence-based treatment recommendations. CI - (c) 2026 International League Against Epilepsy. FAU - Karaahmet, Aysu Yildiz AU - Karaahmet AY AUID- ORCID: 0000-0003-1134-9016 AD - Faculty of Health Sciences, Department of Midwifery, Biruni University, Istanbul, Turkiye. FAU - Bilgic, Fatma Sule AU - Bilgic FS AUID- ORCID: 0000-0002-5950-2553 AD - Faculty of Health Sciences, Department of Midwifery, Canakkale Onsekiz Mart University, Canakkale, Turkiye. FAU - Yuksel, Adnan AU - Yuksel A AUID- ORCID: 0000-0002-7275-8254 AD - Faculty of Medicine, Department of Pediatrics, Biruni University, Istanbul, Turkiye. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Epileptic Disord JT - Epileptic disorders : international epilepsy journal with videotape JID - 100891853 SB - IM OTO - NOTNLM OT - antiseizure medications OT - evidence mapping OT - monotherapy OT - network meta-analysis OT - pediatric focal epilepsy OT - umbrella review EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 10:44 PHST- 2026/09/18 00:00 [revised] PHST- 2026/07/17 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 10:44 [entrez] AID - 10.1002/epd2.70443 [doi] PST - aheadofprint SO - Epileptic Disord. 2026 Oct 28. doi: 10.1002/epd2.70443. PMID- 42949575 OWN - NLM STAT- Publisher LR - 20261028 IS - 1528-1132 (Electronic) IS - 0009-921X (Linking) DP - 2026 Oct 28 TI - What Is the 40-year Survivorship of Periacetabular Osteotomy Free From Conversion to THA? LID - 10.1097/CORR.0000000000004155 [doi] AB - BACKGROUND: Periacetabular osteotomy (PAO) is a widely accepted technique for the surgical treatment of symptomatic acetabular dysplasia with reproducibly favorable short- and midterm outcomes. Long-term follow-up studies are essential because PAO is performed in young patients with the aim of preserving the native hip for many decades. However, follow-up of 30 years remains scarce, and no study has reported 40-year outcomes after PAO. QUESTIONS/PURPOSES: (1) What is the cumulative 40-year survivorship free from conversion to THA? (2) How did hip function change and osteoarthritis progress during follow-up of the surviving hips? (3) Which preoperative factors were associated with conversion to THA? (4) What clinically relevant thresholds can be identified for preoperative factors associated with conversion to THA? METHODS: This was a retrospective case series of a consecutive, single-surgeon cohort followed longitudinally and previously reported at 10, 20, and 30 years. Between 1984 and 1987, a total of 75 hips (63 patients) were treated with PAO for acetabular dysplasia at the Department of Orthopaedic Surgery of the Inselspital Bern by the originator of the procedure. Of those, 57% (43 of 75) of hips had reached the endpoint (conversion to THA or arthrodesis) before our previous report on this group (published at a minimum of 27 years). Over the entire follow-up, 5 hips (4 patients) in which the patient died with the hip preserved were treated as competing events. Since the previous report, a further 8 hips were converted and 8 hips (6 patients) were lost to follow-up, leaving 11 hips (7 patients) with complete clinical and radiographic follow-up here, all at a minimum of 36 years. During the study period (1984 to 1987), the only indication for PAO was hip dysplasia with closed triradiate cartilage. The study group here consisted of 83% (52 of 63) women, the median (range) patient age at surgery was 26 years (12 to 55), and the median (range) BMI was 22 kg/m2 (16 to 28). A total of 27 hips had previous surgery to attempt sufficient coverage. Clinical and radiographic follow-up was performed at 10-year intervals, at 10, 20, 30, and 40 years after PAO. Survivorship free from conversion to THA was estimated using a competing-risks analysis with death without conversion treated as a competing event. Twelve patients underwent bilateral PAO and contributed two hips each. Because two hips from the same patient are not independent observations, all confidence intervals (CIs) and p values were calculated using a robust variance estimator with the patient as the clustering unit. Clinical scores, hip ROM, and osteoarthritis grade were described at each follow-up. Preoperative factors associated with conversion to THA were analyzed using Cox proportional hazards regression with a robust variance estimator clustered on patient. Thresholds for predictive factors were identified using maximally selected rank statistics. This approach tests every observed value of a factor as a potential dividing line and selects the value producing the greatest difference in survivorship between the two resulting groups. RESULTS: At 40 years, the survivorship free from conversion to THA was 27% (95% CI 12% to 39%). Merle d'Aubigne-Postel and Harris hip scores improved during the first decade after surgery and remained above the preoperative level at each follow-up visit. Two preoperative factors were independently associated with a higher risk of conversion to THA: a higher Tonnis osteoarthritis grade (HR 2.3 per 1-grade increase [95% CI 1.4 to 3.9]; p < 0.01) and a positive anterior impingement test result (HR 3.9 versus a negative test result [95% CI 1.9 to 7.7]; p < 0.01). Threshold analysis, which identifies the value of a preoperative factor that best separates hips likely to be preserved from those likely to be converted, identified 28 years for age at surgery. Younger age was therefore associated with a higher likelihood of retaining the native hip. CONCLUSION: PAO provides durable joint preservation in acetabular dysplasia and can delay THA for decades, with 27% of hips remaining free from conversion at 40 years. Preoperative osteoarthritis and a positive anterior impingement test result were associated with conversion to THA. Older age was also associated with conversion, with a threshold of 28 years, although age was related to the degree of joint degeneration at presentation. Patient selection should therefore be based primarily on the preoperative condition of the joint, with younger age at surgery as an additional consideration. LEVEL OF EVIDENCE: Level III, therapeutic study. CI - Copyright (c) 2026 The Author(s). Published by Wolters Kluwer Health, LLC. on behalf of the Association of Bone and Joint Surgeons. FAU - Hirt, Julien AU - Hirt J AUID- ORCID: 0009-0004-4729-1930 AD - Department of Orthopaedic Surgery and Traumatology, Inselspital Bern, University of Bern, Bern, Switzerland. FAU - Roshardt, Jose AU - Roshardt J AUID- ORCID: 0009-0002-6082-0791 FAU - Siebenrock, Klaus A AU - Siebenrock KA AUID- ORCID: 0000-0003-3627-4313 FAU - Ganz, Reinhold AU - Ganz R FAU - Steppacher, Simon D AU - Steppacher SD AUID- ORCID: 0000-0002-4907-8939 FAU - Tannast, Moritz AU - Tannast M AUID- ORCID: 0000-0002-2761-565 LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Clin Orthop Relat Res JT - Clinical orthopaedics and related research JID - 0075674 SB - IM COIS- Each author certifies that there are no funding or commercial associations (consultancies, stock ownership, equity interest, patent/licensing arrangements, etc.) that might pose a conflict of interest in connection with the submitted article related to the author or any immediate family members. All ICMJE Conflict of Interest Forms for authors and Clinical Orthopaedics and Related Research(R) editors and board members are on file with the publication and can be viewed on request. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 10:33 PHST- 2026/05/06 00:00 [received] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 10:33 [entrez] AID - 00003086-990000000-02631 [pii] AID - 10.1097/CORR.0000000000004155 [doi] PST - aheadofprint SO - Clin Orthop Relat Res. 2026 Oct 28. doi: 10.1097/CORR.0000000000004155. PMID- 42949569 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2691-1299 (Electronic) IS - 2691-1299 (Linking) VI - 6 IP - 10 DP - 2026 Oct TI - Effects of PosturePals on Pressure Pain Threshold, Muscle Activity, Craniovertebral Angle, and Quality of Life Among Adults with Cervicothoracic Pain Syndrome: Study Protocol for a Randomized Controlled Trial. LID - e70501 [pii] LID - 10.1002/cpz1.70501 [doi] AB - BACKGROUND: Cervicothoracic pain syndrome (CTPS) is a widespread condition that adversely affects the quality of life (QOL) and is frequently associated with poor posture and muscular imbalance. Despite its prevalence, effective non-invasive interventions remain unexplored. This protocol of a randomized controlled trial (RCT) aims to investigate the effects of PosturePals on pressure pain threshold (PPT), cervicothoracic muscle activity, craniovertebral angle (CVA), and QOL in individuals with CTPS. METHODS: The study will be a two-arm, parallel-group, assessor-blinded randomized-controlled trial involving 58 individuals diagnosed with CTPS, aged 18 to 45 years. Participants will be randomly assigned to two groups: an experimental group receiving PosturePals in addition to conventional physiotherapy, and a control group receiving sham taping and conventional physiotherapy. Outcomes such as PPT (algometry), cervicothoracic muscle activity [surface electromyography (sEMG)], CVA (photographic method), and QOL (SF-36 questionnaires) will be analyzed at baseline (T0), 4 weeks post-intervention (T1), and at 2 weeks follow-up (T2). The study has been approved by the Institutional Ethical Committee of the School of Allied Health Sciences at Galgotias University and registered with the Central Trial Registry of India (CTRI). DISCUSSION: This trial will offer good quality evidence on whether the PosturePals group shows significant improvements in PPT, cervicothoracic muscle activity, CVA, and overall QOL in comparison to the control group. These findings will support the efficacy of PosturePals as a non-invasive therapeutic tool for CTPS, potentially informing clinical practices and guiding future research in musculoskeletal rehabilitation. TRIAL REGISTRATION: CTRI registration number: CTRI/2025/01/079689 registered on 29/01/2025; Ethical Approval: SEC/SAHS/PHD/24/04. (c) 2026 Wiley Periodicals LLC. CI - (c) 2026 Wiley Periodicals LLC. FAU - Malik, Shikha AU - Malik S AUID- ORCID: 0000-0003-0713-838X AD - Department of Physiotherapy, School of Allied Health Sciences, Galgotias University, Greater Noida, India. FAU - Sidiq, Mohammad AU - Sidiq M AUID- ORCID: 0000-0003-2448-8971 AD - Department of Physiotherapy, School of Allied Health Sciences, Galgotias University, Greater Noida, India. FAU - Sharma, Jyoti AU - Sharma J AUID- ORCID: 0000-0002-6510-3903 AD - Department of Physiotherapy, School of Allied Health Sciences, Galgotias University, Greater Noida, India. LA - eng PT - Journal Article PT - Randomized Controlled Trial PL - United States TA - Curr Protoc JT - Current protocols JID - 101773894 SB - IM MH - Humans MH - *Quality of Life MH - Adult MH - *Pain Threshold/physiology MH - Middle Aged MH - Young Adult MH - Adolescent MH - Male MH - *Neck Pain/therapy/physiopathology MH - Female MH - Pressure MH - *Physical Therapy Modalities MH - *Athletic Tape OTO - NOTNLM OT - PosturePals OT - cervicothoracic pain syndrome OT - dynamic taping OT - forward head posture OT - rehabilitation EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 10:33 PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 10:33 [entrez] AID - e70501 [pii] AID - 10.1002/cpz1.70501 [doi] PST - ppublish SO - Curr Protoc. 2026 Oct;6(10):e70501. doi: 10.1002/cpz1.70501. PMID- 42949565 OWN - NLM STAT- Publisher LR - 20261029 IS - 2470-9239 (Electronic) IS - 2470-9239 (Linking) DP - 2026 Oct 28 TI - Clinical efficacy and cost-effectiveness of epilepsy surgery in low- and middle-income countries: A systematic review and meta-analysis. LID - 10.1002/epi4.70376 [doi] AB - OBJECTIVE: To synthesize evidence on clinical outcomes, direct surgical costs, and cost-effectiveness of resective epilepsy surgery for drug-resistant epilepsy in low- and middle-income countries (LMICs). METHODS: MEDLINE, PubMed, Scopus, and the Cochrane Library were searched through May 17, 2026. Original studies from World Bank-classified LMICs reporting clinical outcomes and/or direct costs of resective surgery were included. Engel Class I favorable seizure outcomes at >/= 12 months were pooled using a random-effects model with the Hartung-Knapp-Sidik-Jonkman variance estimator, while cost data were synthesized narratively because of heterogeneity. RESULTS: Ten studies involving 2587 patients from seven LMICs were included from 20 948 identified records. Direct surgical costs ranged from US$500 in Iran to US$14894 in Colombia in 2025-adjusted dollars, equivalent to 9.4%-271% of national GDP per capita. Across four comparative observational studies, surgery was associated with higher odds of achieving Engel Class I favorable seizure outcomes than continued medical management (pooled OR 22.51; 95% CI 11.17-45.38; p < 0.00001; I(2) = 9%). Favorable outcomes occurred in 52%-79.4% of surgical patients versus 7.7%-16% of medically managed patients. Two Thai studies reported conflicting cost-effectiveness results relative to the national willingness-to-pay threshold. SIGNIFICANCE: Resective epilepsy surgery was associated with substantially better seizure outcomes in appropriately selected patients in LMICs. However, the observational clinical evidence and heterogeneous, limited economic data preclude causal interpretation and broad conclusions on cost-effectiveness across settings. PLAIN LANGUAGE SUMMARY: Epilepsy surgery can help people whose seizures remain uncontrolled despite medication, but access to surgery is limited in low- and middle-income countries. This review examined 10 studies involving 2587 patients from seven countries. Patients who underwent surgery were more likely to experience favorable seizure outcomes than those receiving medication alone. Surgical costs varied widely between countries, and evidence about whether surgery provides good value for money remains limited and inconsistent. These findings suggest that expanding access to epilepsy surgery may benefit appropriately selected patients, while further research is needed to understand its affordability and long-term economic value. CI - (c) 2026 The Author(s). Epilepsia Open published by Wiley Periodicals LLC on behalf of International League Against Epilepsy. FAU - Arifin, Muhamad Thohar AU - Arifin MT AUID- ORCID: 0000-0003-2363-8026 AD - Master of Hospital Administration Program, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Yogyakarta, Yogyakarta, Indonesia. AD - Department of Neurosurgery, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia. FAU - Pribadi, Firman AU - Pribadi F AUID- ORCID: 0000-0003-4325-6738 AD - Master Program of Management, Faculty of Economics and Business, Universitas Muhammadiyah Yogyakarta, Yogyakarta, Indonesia. FAU - Oner, Nurettin AU - Oner N AUID- ORCID: 0000-0002-4761-7863 AD - Department of Healthcare Management, Faculty of Health Sciences, Ankara University, Ankara, Turkey. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Epilepsia Open JT - Epilepsia open JID - 101692036 SB - IM PMC - PMC13715675 OTO - NOTNLM OT - cost-effectiveness OT - drug-resistant epilepsy OT - epilepsy surgery OT - low- and middle-income countries OT - seizure outcomes COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/28 CRDT- 2026/10/28 10:23 PHST- 2026/09/04 00:00 [revised] PHST- 2026/07/15 00:00 [received] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 10:23 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - EPI470376 [pii] AID - 10.1002/epi4.70376 [doi] PST - aheadofprint SO - Epilepsia Open. 2026 Oct 28:10.1002/epi4.70376. doi: 10.1002/epi4.70376. PMID- 42949559 OWN - NLM STAT- Publisher LR - 20261028 IS - 2332-4260 (Electronic) IS - 2332-4252 (Linking) DP - 2026 Oct 28 TI - Accuracy, Safety, and Workflow of Frameless Neuronavigation-Guided Stereoelectroencephalography in Drug-Resistant Epilepsy: A Single-Center Cohort Study. LID - 10.1227/ons.0000000000002228 [doi] AB - BACKGROUND AND OBJECTIVES: Stereoelectroencephalography (SEEG) is central to invasive presurgical evaluation in drug-resistant epilepsy (DRE). We evaluated the accuracy, safety, and workflow efficiency of frameless neuronavigation-guided SEEG in routine clinical practice. METHODS: This retrospective single-center cohort included consecutive adult and pediatric patients with DRE who underwent SEEG between July 2016 and August 2025. Entry-point and target-point deviations were measured on postoperative computed tomography fused to the preoperative planning data set. Safety end points were perioperative complications. Operative duration and time per electrode were recorded. Comparative analyses between AdTech and Dixi systems were restricted to the primary-surgery cohort to maintain independent samples. A subgroup analysis examined procedures performed by experienced surgeons, defined as more than 250 implanted electrodes. RESULTS: Seventy-seven patients underwent 82 SEEG procedures. Across all procedures, 984 electrodes were implanted. The median number of electrodes per surgery was 13. The median entry-point and target-point deviations were 2.1 and 1.8 mm, respectively. Entry-point deviation did not differ between AdTech and Dixi electrodes in the primary-surgery cohort (2.1 vs 2.1 mm; P = .579), whereas target-point deviation was slightly lower with Dixi (1.6 vs 1.9 mm; P = .030). The duration of Dixi procedures was shorter than AdTech procedures (102 vs 186 minutes P < .001) and had lower time per electrode (10 vs 14 minutes; P < .001). Four minor hemorrhagic complications occurred (4.9%/procedure; 0.4%/electrode); no permanent neurological deficits, reoperations, hardware-related complications, or procedure-related deaths occurred. A seizure-onset zone was localized in 48 patients (62%), enabling subsequent resective or disconnective surgery. CONCLUSION: Frameless neuronavigation-guided SEEG achieved reproducible millimetric accuracy, low morbidity, and efficient workflow in a consecutive real-world cohort. These findings support frameless neuronavigation as a safe and scalable option for invasive presurgical evaluation in DRE. CI - Copyright (c) Congress of Neurological Surgeons 2026. All rights reserved. FAU - Auer, Christian AU - Auer C AUID- ORCID: 0000-0001-5751-3526 AD - Department of Neurosurgery, Kepler University Hospital GmbH, Johannes Kepler University Linz, Linz, Austria. AD - Clinical Research Institute for Neurosciences, Faculty of Medicine, Johannes Kepler University Linz, Linz, Austria. FAU - Groppel, Gudrun AU - Groppel G AUID- ORCID: 0000-0003-1560-8337 AD - Clinical Research Institute for Neurosciences, Faculty of Medicine, Johannes Kepler University Linz, Linz, Austria. AD - Department of Pediatrics and Adolescent Medicine, Kepler University Hospital GmbH, Johannes Kepler University, Linz, Austria. FAU - Gruber, Andreas AU - Gruber A AUID- ORCID: 0000-0002-0102-1765 AD - Department of Neurosurgery, Kepler University Hospital GmbH, Johannes Kepler University Linz, Linz, Austria. AD - Clinical Research Institute for Neurosciences, Faculty of Medicine, Johannes Kepler University Linz, Linz, Austria. FAU - Hauser, Thomas AU - Hauser T AD - Department of Neurosurgery, Kepler University Hospital GmbH, Johannes Kepler University Linz, Linz, Austria. AD - Clinical Research Institute for Neurosciences, Faculty of Medicine, Johannes Kepler University Linz, Linz, Austria. FAU - Knogler, Katharina AU - Knogler K AD - Department of Pediatrics and Adolescent Medicine, Kepler University Hospital GmbH, Johannes Kepler University, Linz, Austria. FAU - Samueli, Sharon AU - Samueli S AUID- ORCID: 0000-0002-0091-5636 AD - Department of Pediatrics and Adolescent Medicine, Kepler University Hospital GmbH, Johannes Kepler University, Linz, Austria. FAU - Stark, Birgit AU - Stark B AD - Department of Pediatrics and Adolescent Medicine, Kepler University Hospital GmbH, Johannes Kepler University, Linz, Austria. FAU - Sansoni, Gabriela Azevedo AU - Sansoni GA AUID- ORCID: 0000-0003-1751-3921 AD - Department of Pediatrics and Adolescent Medicine, Kepler University Hospital GmbH, Johannes Kepler University, Linz, Austria. FAU - Wach, Katharina AU - Wach K AD - Department of Pediatrics and Adolescent Medicine, Kepler University Hospital GmbH, Johannes Kepler University, Linz, Austria. FAU - Rossmann, Tobias AU - Rossmann T AD - Department of Neurosurgery, Kepler University Hospital GmbH, Johannes Kepler University Linz, Linz, Austria. AD - Clinical Research Institute for Neurosciences, Faculty of Medicine, Johannes Kepler University Linz, Linz, Austria. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Oper Neurosurg JT - Operative neurosurgery (Hagerstown, Md.) JID - 101635417 SB - IM OTO - NOTNLM OT - Accuracy OT - Epilepsy surgery OT - Frameless neuronavigation OT - Presurgical evaluation OT - SEEG EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 10:13 PHST- 2026/05/27 00:00 [received] PHST- 2026/09/13 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 10:13 [entrez] AID - 01787389-990000000-02188 [pii] AID - 10.1227/ons.0000000000002228 [doi] PST - aheadofprint SO - Oper Neurosurg. 2026 Oct 28. doi: 10.1227/ons.0000000000002228. PMID- 42949554 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1539-6924 (Electronic) IS - 0272-4332 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - Information Spillovers Across Media Markets: Risk Perceptions and Cyclospora Outbreaks in U.S. Bagged Salads. LID - e70382 [pii] LID - 10.1111/risa.70382 [doi] AB - From 2018 to 2022, contamination of fresh produce by Cyclospora cayetanensis, primarily affecting bagged salads, caused more than 3,300 outbreak-associated illnesses across the United States. Yet the economic consequences of these outbreaks, and the role of information exposure in shaping consumer responses, remain poorly understood. This study quantifies changes in bagged salad purchases and associated retail losses during six selected large multistate outbreaks occurring from 2018 to 2022 and examines how risk perceptions evolve across outbreak-affected and neighboring regions. Guided by a risk perception framework emphasizing risk proximity and information-driven belief updating, we integrate epidemiologic outbreak data, designated market area (DMA)-level Google Trends search intensity as a geographically granular proxy for information exposure, and NielsenIQ household purchase data. Because DMAs often cross state lines, this design identifies information spillovers to non-outbreak states sharing a media market. A Heckman selection framework accounts for purchase participation and unobserved heterogeneity, while complementary retail scanner data assess whether retailer stocking adjustments contributed to the observed purchase declines. Across the outbreak periods examined in this study, weekly bagged salad purchases declined by an average of 3.89%, implying cumulative retail losses of approximately $373.67 million. Outbreak-specific searches (e.g., "Cyclospora") were associated with greater perceived risk and larger purchase reductions, while general salad-related searches were associated with smaller reductions. Spillovers across shared media markets further reduced purchases in non-outbreak states by 3.33% per week. Retailer analysis found no statistically significant store-level stocking reductions during outbreak or recall periods, supporting the interpretation that the observed purchase declines were primarily demand-driven. These findings highlight the economic significance of information-driven consumer responses and the importance of timely, accurate, and geographically targeted risk communication during foodborne outbreaks. CI - (c) 2026 Society for Risk Analysis. FAU - Yang, Xuerui AU - Yang X AUID- ORCID: 0000-0002-5449-4224 AD - Department of Family, Youth and Community Sciences, University of Florida, Institute of Food and Agricultural Sciences. AD - Department of Human Sciences, The Ohio State University. FAU - Hanks, Andrew S AU - Hanks AS AUID- ORCID: 0000-0003-0687-4772 AD - Department of Human Sciences, The Ohio State University. FAU - Scharff, Robert L AU - Scharff RL AUID- ORCID: 0000-0001-8775-1488 AD - Department of Human Sciences, The Ohio State University. LA - eng PT - Journal Article PL - United States TA - Risk Anal JT - Risk analysis : an official publication of the Society for Risk Analysis JID - 8109978 SB - IM MH - United States/epidemiology MH - *Disease Outbreaks MH - Humans MH - *Cyclospora MH - *Salads/parasitology MH - Risk Assessment MH - *Food Contamination MH - *Cyclosporiasis/epidemiology MH - Commerce EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 10:13 PHST- 2026/09/03 00:00 [revised] PHST- 2025/07/23 00:00 [received] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 10:13 [entrez] AID - e70382 [pii] AID - 10.1111/risa.70382 [doi] PST - ppublish SO - Risk Anal. 2026 Nov;46(11):e70382. doi: 10.1111/risa.70382. PMID- 42949545 OWN - NLM STAT- Publisher LR - 20261028 IS - 1944-8252 (Electronic) IS - 1944-8244 (Linking) DP - 2026 Oct 28 TI - Stomata-Inspired Composite Films for All-Climate Dynamic Thermal Management in High-Humidity Environments. LID - 10.1021/acsami.6c15706 [doi] AB - Passive daytime radiative cooling faces two fatal challenges in high-humidity environments: radiative transmission attenuation in the atmospheric window in summer and overcooling caused by static high emissivity in winter. Existing technologies can only solve one of these problems and cannot achieve all-climate adaptation. Inspired by the dynamic transpiration regulation mechanism of plant stomata, we have fabricated a humidity-responsive cellulose-hydrogel composite film, which autonomously adjusts the pore size of the porous structure via moisture adsorption-desorption to selectively modulate near-infrared reflectance. This film achieves a solar reflectance of 92.0% and an infrared emissivity of 96.1%. At high temperatures, it triggers a synergistic evaporative-radiative cooling effect, realizing a 15.6 degrees C sub-ambient temperature drop and effectively offsetting the cooling performance degradation caused by high humidity; at low temperatures, the material suppresses its radiative cooling power by reducing its solar reflectance. With excellent mechanical properties and weather resistance, it provides a novel design paradigm for all-climate intelligent thermal management in high-humidity regions. CI - (c) 2026 American Chemical Society. FAU - Lv, Zizheng AU - Lv Z AD - School of Chemistry and Chemical Engineering, State Key Laboratory of Green Papermaking and Resource Recycling; Qilu University of Technology (Shandong Academy of Sciences)Jinan250353, P. R. China. FAU - Tao, Furong AU - Tao F AUID- ORCID: 0000-0002-5978-2698 AD - School of Chemistry and Chemical Engineering, State Key Laboratory of Green Papermaking and Resource Recycling; Qilu University of Technology (Shandong Academy of Sciences)Jinan250353, P. R. China. FAU - Ji, Hairui AU - Ji H AUID- ORCID: 0000-0002-8214-0474 AD - School of Chemistry and Chemical Engineering, State Key Laboratory of Green Papermaking and Resource Recycling; Qilu University of Technology (Shandong Academy of Sciences)Jinan250353, P. R. China. FAU - Ji, Xingxiang AU - Ji X AD - School of Chemistry and Chemical Engineering, State Key Laboratory of Green Papermaking and Resource Recycling; Qilu University of Technology (Shandong Academy of Sciences)Jinan250353, P. R. China. FAU - Liu, Libin AU - Liu L AUID- ORCID: 0000-0001-5156-4752 AD - School of Chemistry and Chemical Engineering, State Key Laboratory of Green Papermaking and Resource Recycling; Qilu University of Technology (Shandong Academy of Sciences)Jinan250353, P. R. China. LA - eng GR - 22405145/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261028 PL - United States TA - ACS Appl Mater Interfaces JT - ACS applied materials & interfaces JID - 101504991 SB - IM OTO - NOTNLM OT - anti-overcooling OT - cellulose OT - evaporative cooling OT - radiative cooling OT - thermal regulation EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 10:03 PHST- 2026/07/30 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 10:03 [entrez] AID - 5460073 [pii] AID - 10.1021/acsami.6c15706 [doi] PST - aheadofprint SO - ACS Appl Mater Interfaces. 2026 Oct 28. doi: 10.1021/acsami.6c15706. PMID- 42949541 OWN - NLM STAT- Publisher LR - 20261028 IS - 1099-1573 (Electronic) IS - 0951-418X (Linking) DP - 2026 Oct 28 TI - 4'-Hydroxywogonin Attenuates Hepatocellular Carcinoma Progression by Suppressing Gas6/Axl-PI3K/AKT Signaling. LID - 10.1002/ptr.70499 [doi] AB - 4'-Hydroxywogonin, a bioactive flavonoid derived from Scutellaria baicalensis, has reported anti-inflammatory and anticancer activities; however, its therapeutic potential and molecular mechanism in hepatocellular carcinoma (HCC) remain unclear. This study investigated the anti-tumor effects of 4'-hydroxywogonin and its regulatory role in Gas6/Axl-associated signaling. Human HCC cells were used to evaluate proliferation, migration, invasion, cell cycle distribution, apoptosis, senescence, and epithelial-mesenchymal transition. Whole-transcriptome sequencing, western blotting, quantitative polymerase chain reaction, molecular docking, and thermal shift analysis were performed to explore the underlying mechanism, and antitumor efficacy and safety were further examined in a mouse xenograft model. 4'-Hydroxywogonin significantly inhibited HCC cell proliferation, migration, and invasion, while inducing G1-phase arrest, apoptosis, and senescence. Transcriptomic and molecular analyses identified Axl as a key affected target. 4'-Hydroxywogonin reduced Gas6 and Axl expression, suppressed PI3K/AKT activation, and downregulated cell cycle and epithelial-mesenchymal transition (EMT)-related proteins. Molecular docking predicted a potential interaction between 4'-hydroxywogonin and Axl, which was supported by thermal shift analysis. Axl overexpression partially rescued the inhibitory effects of 4'-hydroxywogonin, suggesting involvement of Axl-dependent and additional pathways. In vivo, 4'-hydroxywogonin markedly reduced tumor growth with efficacy comparable to sorafenib and without evident hepatotoxicity. These findings indicate that 4'-hydroxywogonin suppresses HCC progression, at least in part, through inhibition of the Gas6/Axl-PI3K/AKT signaling axis, supporting its further development as a natural therapeutic candidate. CI - (c) 2026 The Author(s). Phytotherapy Research published by John Wiley & Sons Ltd. FAU - Chen, Chi-Yuan AU - Chen CY AUID- ORCID: 0000-0003-4995-297X AD - Biobank, Chang Gung Memorial Hospital, Taoyuan, Taiwan. AD - Graduate Institute of Health Industry and Technology, Research Center for Food and Cosmetic Safety, Chang Gung University of Science and Technology, Taoyuan, Taiwan. AD - Institute of Oral Biology, College of Dentistry, National Yang Ming Chiao Tung University, Taipei, Taiwan. FAU - Chen, Chin-Chuan AU - Chen CC AD - Biobank, Chang Gung Memorial Hospital, Taoyuan, Taiwan. AD - Graduate Institute of Natural Products, Chang Gung University, Taoyuan, Taiwan. FAU - Yeh, Chau-Ting AU - Yeh CT AD - Liver Research Center, Department of Hepato-Gastroenterology, Chang Gung Memorial Hospital, Taoyuan, Taiwan. FAU - Chan, Chieh-Wen AU - Chan CW AD - Graduate Institute of Health Industry and Technology, Research Center for Food and Cosmetic Safety, Chang Gung University of Science and Technology, Taoyuan, Taiwan. FAU - Chu, Yu-De AU - Chu YD AUID- ORCID: 0000-0002-5496-8573 AD - Liver Research Center, Department of Hepato-Gastroenterology, Chang Gung Memorial Hospital, Taoyuan, Taiwan. FAU - Leu, Yann-Lii AU - Leu YL AD - Biobank, Chang Gung Memorial Hospital, Taoyuan, Taiwan. AD - Graduate Institute of Natural Products, Chang Gung University, Taoyuan, Taiwan. FAU - Satange, Roshan B AU - Satange RB AUID- ORCID: 0000-0002-5150-9363 AD - Biobank, Chang Gung Memorial Hospital, Taoyuan, Taiwan. AD - Graduate Institute of Natural Products, Chang Gung University, Taoyuan, Taiwan. FAU - Chou, Li-Fang AU - Chou LF AUID- ORCID: 0000-0002-0189-1699 AD - Kidney Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan. FAU - Chuang, Wen-Yu AU - Chuang WY AUID- ORCID: 0000-0002-3729-073X AD - Department of Pathology, Cathay General Hospital, Taipei, Taiwan. FAU - Hsueh, Chuen AU - Hsueh C AD - Department of Pathology, Cathay General Hospital, Taipei, Taiwan. FAU - Shieh, Tzong-Ming AU - Shieh TM AUID- ORCID: 0000-0003-1759-9120 AD - Institute of Oral Biology, College of Dentistry, National Yang Ming Chiao Tung University, Taipei, Taiwan. AD - School of Dentistry, College of Dentistry, China Medical University, Taichung, Taiwan. FAU - Wang, Tong-Hong AU - Wang TH AUID- ORCID: 0000-0001-9248-9031 AD - Biobank, Chang Gung Memorial Hospital, Taoyuan, Taiwan. AD - Graduate Institute of Health Industry and Technology, Research Center for Food and Cosmetic Safety, Chang Gung University of Science and Technology, Taoyuan, Taiwan. AD - Graduate Institute of Natural Products, Chang Gung University, Taoyuan, Taiwan. AD - Liver Research Center, Department of Hepato-Gastroenterology, Chang Gung Memorial Hospital, Taoyuan, Taiwan. LA - eng GR - 111-2320-B-182A-011/National Science and Technology Council/ GR - 112-2320-B-182A-009-MY3/National Science and Technology Council/ GR - 115-2320-B-182A-009-MY3/National Science and Technology Council/ GR - CMRPG3N0512/Chang Gung Medical Foundation/ GR - CMRPG3N0513/Chang Gung Medical Foundation/ GR - CMU112-S-51/China Medical University, Taiwan/ PT - Journal Article DEP - 20261028 PL - England TA - Phytother Res JT - Phytotherapy research : PTR JID - 8904486 SB - IM OTO - NOTNLM OT - 4'-hydroxywogonin OT - Gas6/Axl signaling OT - PI3K/AKT OT - apoptosis OT - epithelial-mesenchymal transition (EMT) OT - hepatocellular carcinoma (HCC) EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 10:03 PHST- 2026/10/13 00:00 [revised] PHST- 2026/06/18 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 10:03 [entrez] AID - 10.1002/ptr.70499 [doi] PST - aheadofprint SO - Phytother Res. 2026 Oct 28. doi: 10.1002/ptr.70499. PMID- 42949538 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1477-4054 (Electronic) IS - 1467-5463 (Print) IS - 1467-5463 (Linking) VI - 27 IP - 5 DP - 2026 Sep 1 TI - TRACER: response-protected cross-domain alignment of cancer pharmacogenomic models with patient tumors. LID - 10.1093/bib/bbag593 [doi] LID - bbag593 AB - Pharmacogenomics integrates tumor molecular features with drug responses to support precision oncology, but biological domain shifts and optimization conflicts hinder transfer from screened cell lines to unlabeled patient tumors. We developed TRACER (Transfer of Response Across domains via Conflict-aware End-to-end Regulation), an end-to-end framework combining supervised drug-response prediction, biologically conditioned neighbor alignment and response-protected gradient regulation. By projecting only conflicting components of the auxiliary alignment gradient, TRACER preserves the response-optimization direction learned from experimental labels. Across ModelID-disjoint, seen-drug splits, TRACER-Full achieved a global Pearson correlation of 0.5125 $\pm $ 0.0192 on per-drug z-standardized test pairs, compared with 0.5179 $\pm $ 0.0193 for Response-only, a mean difference of $-0.0054$. Mean oncogenic Hallmark neighbor consistency increased by $0.1162 \pm 0.0210$ in alignment-excluded The Cancer Genome Atlas audit samples. At the patient level, a source-validation-defined drug partition and uniform stability analysis supported four predicted pharmacological response subtypes (PRSs) in breast invasive carcinoma (BRCA), four in uterine corpus endometrial carcinoma (UCEC) and two in sarcoma (SARC). PRS-construction-excluded drugs characterized distinct predicted mechanism-level profiles, and pan-cancer indication recovery provided a lineage-aware internal consistency assessment of the patient rankings. TRACER provides an interpretable and auditable framework for response-preserving cross-domain alignment and it generates patient pharmacogenomic hypotheses for subsequent functional validation. CI - (c) The Author(s) 2026. Published by Oxford University Press. FAU - Wang, Zhenxing AU - Wang Z AUID- ORCID: 0009-0002-1123-6812 AD - College of Computer Science and Artificial Intelligence, Northeast Forestry University, No. 26 Hexing Road, Xiangfang District, Harbin, Heilongjiang 150040, China. FAU - Wang, Qihe AU - Wang Q AUID- ORCID: 0009-0006-0210-5350 AD - College of Computer Science and Artificial Intelligence, Northeast Forestry University, No. 26 Hexing Road, Xiangfang District, Harbin, Heilongjiang 150040, China. FAU - Wang, Guangchao AU - Wang G AUID- ORCID: 0009-0002-0816-9313 AD - College of Computer Science and Artificial Intelligence, Northeast Forestry University, No. 26 Hexing Road, Xiangfang District, Harbin, Heilongjiang 150040, China. FAU - Wang, Guohua AU - Wang G AUID- ORCID: 0000-0001-7381-2374 AD - College of Computer Science and Artificial Intelligence, Northeast Forestry University, No. 26 Hexing Road, Xiangfang District, Harbin, Heilongjiang 150040, China. AD - Department of Computer Science and Technology, Faculty of Computing, Harbin Institute of Technology, No. 92 West Dazhi Street, Nangang District, Harbin, Heilongjiang 150001, China. FAU - Zhao, Yuming AU - Zhao Y AUID- ORCID: 0000-0001-7219-0999 AD - College of Computer Science and Artificial Intelligence, Northeast Forestry University, No. 26 Hexing Road, Xiangfang District, Harbin, Heilongjiang 150040, China. LA - eng GR - 62302100/National Natural Science Foundation of China/ GR - 62225109/National Science Fund for Distinguished Young Scholars/ GR - ZD2024F001/Key Project of the Natural Science Foundation of Heilongjiang Province/ GR - 2572025BR13/Fundamental Research Funds for the Central Universities/ PT - Journal Article PL - England TA - Brief Bioinform JT - Briefings in bioinformatics JID - 100912837 RN - 0 (Antineoplastic Agents) SB - IM MH - Humans MH - *Pharmacogenetics/methods MH - *Neoplasms/genetics/drug therapy MH - *Antineoplastic Agents/therapeutic use/pharmacology MH - Female MH - Algorithms PMC - PMC13714793 OTO - NOTNLM OT - cancer pharmacogenomics OT - domain adaptation OT - gradient conflict OT - multitask learning OT - pharmacological response subtyping OT - representation alignment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/28 CRDT- 2026/10/28 10:03 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 10:03 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 9003614 [pii] AID - bbag593 [pii] AID - 10.1093/bib/bbag593 [doi] PST - ppublish SO - Brief Bioinform. 2026 Sep 1;27(5):bbag593. doi: 10.1093/bib/bbag593. PMID- 42949536 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1477-4054 (Electronic) IS - 1467-5463 (Print) IS - 1467-5463 (Linking) VI - 27 IP - 5 DP - 2026 Sep 1 TI - Advances in computational biology approaches for digital modeling of tumor microenvironment. LID - 10.1093/bib/bbag595 [doi] LID - bbag595 AB - Tumor progression arises from tightly coupled cellular, vascular, metabolic, immune, and biomechanical processes within the tumor microenvironment (TME), forming a heterogeneous multiscale system that cannot be fully characterized through experimental observation alone. Computational modeling provides a quantitative framework for integrating these mechanisms, but the diversity of available methods complicates model selection, parameterization, validation, and clinical translation. This review presents a problem-driven synthesis of computational approaches for TME modeling. Continuous, discrete, and discrete-continuous hybrid paradigms are compared in terms of biological resolution, data requirements, interpretability, and computational cost, and are linked to five mechanistic domains: tumor-cell dynamics, vascular transport, extracellular matrix and mechanics, metabolism and hypoxia, and immune interactions. Model credibility is examined through parameter identifiability, uncertainty quantification, numerical validity, biological fidelity, and clinical relevance, with emphasis on interpreting performance according to intended use and validation context. Machine learning is analyzed by function within mechanistic-ML integration: surrogate models accelerate forward simulation, inverse methods estimate latent parameters and states, patient-specific workflows combine multimodal clinical data with mechanistic models, and physics-informed neural networks embed governing equations into learning. We further discuss longitudinal personalization, physics-aware multiscale integration, clinically deployable digital twins, external generalization, and synthetic-data-to-clinical transfer. A translation-readiness pathway is proposed from mechanistic hypothesis generation to adaptive digital-twin decision support. Credible TME modeling should prioritize the minimum sufficient model, explicit intended use, transparent uncertainty, and evidence matched to the biological or clinical question. CI - (c) The Author(s) 2026. Published by Oxford University Press. FAU - Ming, Wenlong AU - Ming W AUID- ORCID: 0000-0002-5553-905X AD - Jiangsu Key Laboratory of Intelligent Medical Image Computing, School of Artificial Intelligence, Nanjing University of Information Science and Technology, 219 Ning Liu Road, Nanjing, Jiangsu Province 210044, China. FAU - Lu, Huimin AU - Lu H AD - Jiangsu Key Laboratory of Intelligent Medical Image Computing, School of Artificial Intelligence, Nanjing University of Information Science and Technology, 219 Ning Liu Road, Nanjing, Jiangsu Province 210044, China. FAU - Zeng, Tianhao AU - Zeng T AD - Jiangsu Key Laboratory of Intelligent Medical Image Computing, School of Artificial Intelligence, Nanjing University of Information Science and Technology, 219 Ning Liu Road, Nanjing, Jiangsu Province 210044, China. FAU - Luo, Kun AU - Luo K AD - Department of Neurosurgery, The First Affiliated Hospital of Xinjiang Medical University, No. 137 Liyushan South Road, Xinshi District, Urumqi, Xinjiang Uygur Autonomous Region 830054, China. FAU - Xu, Jun AU - Xu J AD - Jiangsu Key Laboratory of Intelligent Medical Image Computing, School of Artificial Intelligence, Nanjing University of Information Science and Technology, 219 Ning Liu Road, Nanjing, Jiangsu Province 210044, China. FAU - Liu, Hongde AU - Liu H AUID- ORCID: 0000-0001-8768-764X AD - State Key Laboratory of Digital Medical Engineering, School of Biological Science & Medical Engineering, Southeast University, No. 2 Sipailou, Xuanwu District, Nanjing, Jiangsu Province 210096, China. LA - eng PT - Journal Article PT - Review PL - England TA - Brief Bioinform JT - Briefings in bioinformatics JID - 100912837 SB - IM MH - Humans MH - *Tumor Microenvironment MH - *Computational Biology/methods MH - *Neoplasms/pathology/metabolism MH - *Models, Biological MH - Computer Simulation MH - Machine Learning MH - Soft Computing PMC - PMC13714792 OTO - NOTNLM OT - digital twins OT - discrete-continuous hybrid modeling OT - mechanistic-machine learning integration OT - multiscale modeling OT - patient-specific modeling OT - tumor microenvironment COIS- None declared. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/28 CRDT- 2026/10/28 10:03 PHST- 2026/04/03 00:00 [received] PHST- 2026/08/19 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 10:03 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 9003611 [pii] AID - bbag595 [pii] AID - 10.1093/bib/bbag595 [doi] PST - ppublish SO - Brief Bioinform. 2026 Sep 1;27(5):bbag595. doi: 10.1093/bib/bbag595. PMID- 42949532 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1435-8115 (Electronic) IS - 1431-9276 (Linking) VI - 32 IP - 6 DP - 2026 Oct 12 TI - Image Drift Under High-Power Laser Irradiation in a Side-Entry Fiber-Optic TEM Holder. LID - ozag113 [pii] LID - 10.1093/mam/ozag113 [doi] AB - In situ laser irradiation in a transmission electron microscope (TEM) enables direct observation of laser-induced microstructural evolution. A side-entry TEM holder with an optical fiber has been fabricated for 808 nm continuous-wave near-infrared irradiation, and the apparent image-drift behavior under high-power laser exposure has been investigated. Support-free Au, Cu, Ni, and stainless steel (SS) grids were first examined using a 30 s laser-on/30 s laser-off protocol. The image-drift magnitude followed the order SS > Ni > Cu approximately Au, with rapid switching displacement followed by a continued drift at a progressively decreasing rate. To investigate the effect of support materials on the image drift, Au and Cu grids with lacey carbon and continuous carbon films were also evaluated. For both metals, image drift consistently increased in the order: support-free < lacey carbon < continuous carbon film, reflecting the combined effects of optical absorption, thermal transport, and mechanical constraint. Infrared thermography under vacuum further confirmed material-dependent heating, consistent with the thermoelastic interpretation of the measured drift. The Cu grid showed no visible damage under irradiation of /=3 times/week), seafood (1-2 times/week) and vegetables (>/=3 times/week) was associated with lower odds of ever wheezing (meat: AOR = 0.27, 95% CI: 0.10-0.75; seafood: AOR = 0.24, 95% CI: 0.13-0.46; vegetables: AOR = 0.32, 95% CI: 0.11-0.93), while no clear associations were observed for current wheeze.SummaryHigher consumption of meat, seafood and vegetables was linked to lower odds of ever wheezing in Tshwane preschool children. Promoting nutrient-rich dietary patterns may help prevent early-life respiratory symptoms in underserved communities. FAU - Bhuda, Mandla AU - Bhuda M AUID- ORCID: 0009-0001-2468-019X AD - Department of Health Studies, University of South Africa, Pretoria, South Africa. RINGGOLD: 56866 AD - Faculty of Health Sciences, School of Health Systems and Public Health, University of Pretoria, Pretoria, South Africa. FAU - Wichmann, Janine AU - Wichmann J AD - Faculty of Health Sciences, School of Health Systems and Public Health, University of Pretoria, Pretoria, South Africa. FAU - Shirinde, Joyce AU - Shirinde J AD - Faculty of Health Sciences, School of Health Systems and Public Health, University of Pretoria, Pretoria, South Africa. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Nutr Health JT - Nutrition and health JID - 8306569 SB - IM OTO - NOTNLM OT - Tshwane OT - Wheezing OT - asthma OT - dietary patterns OT - preschool children EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 09:53 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:53 [entrez] AID - 10.1177/02601060261502311 [doi] PST - aheadofprint SO - Nutr Health. 2026 Oct 28:2601060261502311. doi: 10.1177/02601060261502311. PMID- 42949521 OWN - NLM STAT- Publisher LR - 20261028 IS - 2638-6100 (Electronic) IS - 2638-6100 (Linking) DP - 2026 Oct 28 TI - Anatomy-informed CT Spatial Phenotype Associated with Extrapancreatic Perineural Invasion in Resected Pancreatic Ductal Adenocarcinoma. PG - e260357 LID - 10.1148/ryai.260357 [doi] AB - Purpose To develop and evaluate an interpretable CT spatial phenotype associated with extrapancreatic perineural invasion (EPNI) in resected pancreatic ductal adenocarcinoma. Materials and Methods This multicenter study included training, internal testing, neoadjuvant testing, retrospective external testing, prospective external testing, and The Cancer Imaging Archive (TCIA) sets. The Preoperative Risk of Perineural High-risk Extension in Tumors (PROPHET) model and threshold were locked before testing. Performance was assessed with area under the receiver operating characteristic curve (AUC) and threshold metrics. Six radiologists compared unaided and assisted readings. A stratified Cox model related PROPHET risk to survival, adjusting for age, sex, tumor size, carbohydrate antigen 19-9, nodal category, and differentiation. Results Among 1854 patients (median age, 63 years; 1131 male), AUCs were 0.92 (95% CI, 0.86-0.96) in prospective external testing, 0.85 (95% CI, 0.75-0.93) in retrospective external testing, 0.90 (95% CI, 0.83-0.95) after neoadjuvant therapy, 0.99 (95% CI, 0.98-> 0.99) in internal testing, and 0.80 (95% CI, 0.68-0.93) in TCIA. TCIA sensitivity and specificity were 24/30 (80%) and 2/19 (11%). Assistance increased mean sensitivity to 93% from 82% and reduced reading time to 3.02 from 3.64 minutes per patient (both Holm-adjusted P < .001). Higher PROPHET risk was associated with shorter survival (adjusted hazard ratio, 1.95; 95% CI, 1.53-2.47; nominal P < .001). Conclusion PROPHET provides a CT-visible tumor-vessel spatial phenotype associated with pathologically confirmed EPNI. (c) The Author(s) 2026. Published by the Radiological Society of North America under a CC BY 4.0 license. FAU - Chen, Chengwei AU - Chen C AUID- ORCID: 0000-0002-8167-7464 AD - Department of Radiology, Changhai Hospital, 168 Changhai Road, Shanghai 200433, China. FAU - Yu, Jieyu AU - Yu J AUID- ORCID: 0000-0002-8701-7440 AD - Department of Radiology, Changhai Hospital, 168 Changhai Road, Shanghai 200433, China. FAU - Shen, Yixuan AU - Shen Y AD - Department of Radiology, Changhai Hospital, 168 Changhai Road, Shanghai 200433, China. FAU - Zheng, Danqun AU - Zheng D AUID- ORCID: 0009-0004-1154-5169 AD - Department of Radiology, Changhai Hospital, 168 Changhai Road, Shanghai 200433, China. FAU - Zheng, Yilun AU - Zheng Y AD - Department of Radiology, Changhai Hospital, 168 Changhai Road, Shanghai 200433, China. FAU - Liu, Fang AU - Liu F AD - Department of Radiology, Changhai Hospital, 168 Changhai Road, Shanghai 200433, China. FAU - Wang, Tiegong AU - Wang T AD - Department of Radiology, Changhai Hospital, 168 Changhai Road, Shanghai 200433, China. FAU - Fang, Xu AU - Fang X AUID- ORCID: 0000-0001-5795-9843 AD - Department of Radiology, Changhai Hospital, 168 Changhai Road, Shanghai 200433, China. FAU - Li, Jing AU - Li J AD - Department of Radiology, Changhai Hospital, 168 Changhai Road, Shanghai 200433, China. FAU - Lu, Jianping AU - Lu J AUID- ORCID: 0000-0003-1393-9212 AD - Department of Radiology, Changhai Hospital, 168 Changhai Road, Shanghai 200433, China. FAU - Jiang, Hui AU - Jiang H AUID- ORCID: 0000-0003-3972-1297 AD - Department of Pathology, Changhai Hospital, Shanghai, China. FAU - Shao, Chengwei AU - Shao C AUID- ORCID: 0000-0003-1640-9663 AD - Department of Radiology, Changhai Hospital, 168 Changhai Road, Shanghai 200433, China. FAU - Bian, Yun AU - Bian Y AUID- ORCID: 0000-0002-4863-4956 AD - Department of Radiology, Changhai Hospital, 168 Changhai Road, Shanghai 200433, China. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Radiol Artif Intell JT - Radiology. Artificial intelligence JID - 101746556 SB - IM EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 09:53 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:53 [entrez] AID - 10.1148/ryai.260357 [doi] PST - aheadofprint SO - Radiol Artif Intell. 2026 Oct 28:e260357. doi: 10.1148/ryai.260357. PMID- 42949514 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 0009-4293 (Print) IS - 0009-4293 (Linking) VI - 80 IP - 10 DP - 2026 Oct 28 TI - mTOR and Extracellular Vesicles: Linking Chemical Stress to Ecological Communication. PG - 685-694 LID - 10.2533/chimia.2026.685 [doi] AB - Environmental stress, including that exerted by exposure to toxic chemicals, can shift cellular priorities from growth towards maintenance and communication. These changes in cellular resource allocation are coordinated by integrative molecular hubs such as the mechanistic Target of Rapamycin (mTOR) pathway. It regulates both the autophagy, which supports maintenance, and the release of extracellular vesicles (EVs), which mediate intercellular communication. This review discusses these interconnected processes and argues that environmental contaminants may influence not only intracellular signaling but also the molecular information exchanged among cells and potentially among organisms. EV-focused studies, performed at the intersection of chemical ecology, molecular biology, and ecotoxicology, can advance mechanistic understanding and open new opportunities for non-invasive environmental biomonitoring. CI - Copyright 2026 Ksenia J. Groh. License: This work is licensed under a Creative Commons Attribution 4.0 International License. FAU - Groh, Ksenia J AU - Groh KJ AD - Department of Environmental Toxicology, Eawag - Swiss Federal Institute of Aquatic Science and Technology, CH-8600 Dubendorf. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - Switzerland TA - Chimia (Aarau) JT - Chimia JID - 0373152 RN - EC 2.7.11.1 (TOR Serine-Threonine Kinases) RN - 0 (Environmental Pollutants) SB - IM MH - *TOR Serine-Threonine Kinases/metabolism MH - *Extracellular Vesicles/metabolism/drug effects MH - *Stress, Physiological MH - Humans MH - Animals MH - Signal Transduction MH - *Environmental Pollutants/toxicity OTO - NOTNLM OT - Chemical pollution OT - Environmental monitoring OT - Extracellular vesicle OT - Mechanistic Target of Rapamycin (mTOR) EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 09:53 PHST- 2026/07/14 00:00 [received] PHST- 2026/09/10 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:53 [entrez] AID - 10.2533/chimia.2026.685 [doi] PST - epublish SO - Chimia (Aarau). 2026 Oct 28;80(10):685-694. doi: 10.2533/chimia.2026.685. PMID- 42949511 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 0009-4293 (Print) IS - 0009-4293 (Linking) VI - 80 IP - 10 DP - 2026 Oct 28 TI - Emerging Technologies for the Discovery and Characterization of Microbial Natural Products. PG - 664-670 LID - 10.2533/chimia.2026.664 [doi] AB - The discovery of natural products has changed dramatically in recent years, and new technologies have been developed continuously to improve this process, including the integration of artificial intelligence tools. Herein, we summarize some emerging methodologies, including genome mining, activation of selected biosynthetic pathways, chemical probes, and mass spectrometry-based metabolomics. Selected examples are discussed in detail, highlighting and describing their discovery strategies. CI - Copyright 2026 Simon Sieber. License: This work is licensed under a Creative Commons Attribution 4.0 International License. FAU - Sieber, Simon AU - Sieber S AD - Department of Chemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - Switzerland TA - Chimia (Aarau) JT - Chimia JID - 0373152 RN - 0 (Biological Products) SB - IM MH - *Biological Products/chemistry/metabolism MH - *Drug Discovery/methods MH - Metabolomics MH - Mass Spectrometry OTO - NOTNLM OT - Drug Discovery OT - Genome mining OT - Metabolomics OT - Natural products EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 09:53 PHST- 2026/06/15 00:00 [received] PHST- 2026/08/27 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:53 [entrez] AID - 10.2533/chimia.2026.664 [doi] PST - epublish SO - Chimia (Aarau). 2026 Oct 28;80(10):664-670. doi: 10.2533/chimia.2026.664. PMID- 42949508 OWN - NLM STAT- Publisher LR - 20261028 IS - 1600-0722 (Electronic) IS - 0909-8836 (Linking) DP - 2026 Oct 28 TI - Time-to-transformation transcriptomic modeling identifies oral squamous cell carcinoma-initiation and SPP1-integrin niche programs in oral leukoplakia. LID - e70157 [pii] LID - 10.1111/eos.70157 [doi] AB - Oral leukoplakia and other oral potentially malignant disorders carry heterogeneous risks of subsequent oral squamous cell carcinoma (OSCC), whereas conventional clinicopathological factors have limited ability to discriminate individual risk and event timing. We developed a time-to-transformation transcriptomic score (TT-score) in the longitudinal GSE26549 cohort, including 86 participants and 35 subsequent oral-cancer events. A 12-gene TT-score was derived using multimethod feature selection and Cox-GLMNET modeling, and the full development pipeline was evaluated by 20 repeated five-fold nested cross-validations. Strict internal validation yielded a mean out-of-fold C-index of 0.740 (95% confidence interval, 0.679-0.801), with mean time-dependent area under the curve (AUCs) of 0.731, 0.771, and 0.811 at 2-, 3-, and 5-yr, respectively. Full-pipeline outcome permutation analysis supported the non-random nature of the observed risk-ranking signal. Across external progression-axis cohorts, direct stage associations of the fixed TT-score were cohort-dependent, whereas TT-score-related epithelial proliferation, invasion, hypoxia/metabolic, OSCC-initiation-like, and SPP1-related microenvironmental programs showed more consistent stage-associated trends. Single-cell analyses and published spatial-atlas signatures further contextualized these programs. Overall, the TT-score captured a transcriptomic risk state associated with the timing of subsequent oral cancer and provides a candidate framework for independent longitudinal validation and targeted assay development. CI - (c) 2026 Scandinavian Division of the International Association for Dental Research. Published by John Wiley & Sons Ltd. FAU - Guo, Zhengze AU - Guo Z AUID- ORCID: 0009-0006-4831-2304 AD - Department of Stomatology, The 81st Group Army Hospital of the Chinese People's Liberation Army, Zhangjiakou, Hebei, China. FAU - Shi, Yong AU - Shi Y AUID- ORCID: 0009-0007-9479-7450 AD - Department of Stomatology, The 81st Group Army Hospital of the Chinese People's Liberation Army, Zhangjiakou, Hebei, China. FAU - Zhang, Xianan AU - Zhang X AUID- ORCID: 0009-0002-3421-7884 AD - Department of Stomatology, The 81st Group Army Hospital of the Chinese People's Liberation Army, Zhangjiakou, Hebei, China. FAU - Wu, QiuYue AU - Wu Q AD - Department of Stomatology, The 81st Group Army Hospital of the Chinese People's Liberation Army, Zhangjiakou, Hebei, China. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Eur J Oral Sci JT - European journal of oral sciences JID - 9504563 SB - IM OTO - NOTNLM OT - Cox-GLMNET OT - SPP1 OT - literature-atlas signatures OT - nested cross-validation OT - oral leukoplakia OT - oral potentially malignant disorders OT - risk modeling OT - single-cell RNA sequencing OT - subsequent oral cancer OT - time-to-transformation transcriptomic score EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 09:53 PHST- 2026/06/21 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:53 [entrez] AID - e70157 [pii] AID - 10.1111/eos.70157 [doi] PST - aheadofprint SO - Eur J Oral Sci. 2026 Oct 28:e70157. doi: 10.1111/eos.70157. PMID- 42949483 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1079-7114 (Electronic) IS - 0031-9007 (Linking) VI - 137 IP - 15 DP - 2026 Oct 9 TI - Two-Dimensional Transverse-Momentum Subtraction and Semi-Inclusive Deep-Inelastic Scattering at Next-to-Next-to-Next-to-Leading Order in QCD. PG - 151901 LID - 10.1103/gnk4-8p43 [doi] AB - Identified hadron production is essential for the study of nucleon structure and QCD hadronization at high energies. We present the first calculation of unpolarized semi-inclusive deep-inelastic scattering at next-to-next-to-next-to-leading order in perturbative QCD. Our calculation is based on a novel method of two-dimensional transverse-momentum subtraction motivated by QCD factorization of soft and collinear singularities. The next-to-next-to-next-to-leading order corrections are moderate in general but can be significant in threshold regions, and exhibit excellent perturbative convergence and reduced scale variations. The fully differential framework allows for arbitrary selection cuts and directly enables precision nucleon tomography at the upcoming Electron-Ion Collider, establishing the theory foundation needed to match the anticipated experimental accuracy. Generalization of the method to calculations of polarized semi-inclusive deep-inelastic scattering is also feasible. FAU - Dong, Liang AU - Dong L AD - Shanghai Jiao Tong University, State Key Laboratory of Dark Matter Physics, Shanghai Key Laboratory for Particle Physics and Cosmology, Key Laboratory for Particle Astrophysics and Cosmology (MOE), School of Physics and Astronomy, Shanghai 200240, China. ROR: https://ror.org/0220qvk04 FAU - Fang, Shen AU - Fang S AD - Fudan University, Department of Physics, Center for Field Theory and Particle Physics, and Key Laboratory of Nuclear Physics and Ion-beam Application (MOE), Shanghai, 200433, China. ROR: https://ror.org/013q1eq08 FAU - Gao, Jun AU - Gao J AD - Shanghai Jiao Tong University, State Key Laboratory of Dark Matter Physics, Shanghai Key Laboratory for Particle Physics and Cosmology, Key Laboratory for Particle Astrophysics and Cosmology (MOE), School of Physics and Astronomy, Shanghai 200240, China. ROR: https://ror.org/0220qvk04 FAU - Li, Hai Tao AU - Li HT AD - Shandong University, School of Physics, Jinan, Shandong 250100, China. ROR: https://ror.org/0207yh398 FAU - Shao, Ding Yu AU - Shao DY AD - Fudan University, Department of Physics, Center for Field Theory and Particle Physics, and Key Laboratory of Nuclear Physics and Ion-beam Application (MOE), Shanghai, 200433, China. ROR: https://ror.org/013q1eq08 AD - Fudan University, Shanghai Research Center for Theoretical Nuclear Physics, NSFC and , Shanghai 200438, China. ROR: https://ror.org/013q1eq08 AD - Peking University, Center for High Energy Physics, Beijing 100871, China. ROR: https://ror.org/02v51f717 AD - Institute of Modern Physics, Southern Center for Nuclear-Science Theory (SCNT), Chinese Academy of Sciences, Huizhou 516000, Guangdong Province, China. ROR: https://ror.org/03x8rhq63 FAU - Zhu, Hua Xing AU - Zhu HX AD - Peking University, Center for High Energy Physics, Beijing 100871, China. ROR: https://ror.org/02v51f717 AD - Institute of Modern Physics, Southern Center for Nuclear-Science Theory (SCNT), Chinese Academy of Sciences, Huizhou 516000, Guangdong Province, China. ROR: https://ror.org/03x8rhq63 AD - Peking University, School of Physics, Beijing 100871, China. ROR: https://ror.org/02v51f717 FAU - Zhu, Yu Jiao AU - Zhu YJ AD - Max-Planck-Institut fur Physik, Werner-Heisenberg-Institut, Boltzmannstrasse 8, 85748 Garching, Germany. ROR: https://ror.org/0079jjr10 LA - eng PT - Journal Article PL - United States TA - Phys Rev Lett JT - Physical review letters JID - 0401141 SB - IM EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 09:43 PHST- 2026/04/12 00:00 [received] PHST- 2026/08/03 00:00 [revised] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:43 [entrez] AID - 10.1103/gnk4-8p43 [doi] PST - ppublish SO - Phys Rev Lett. 2026 Oct 9;137(15):151901. doi: 10.1103/gnk4-8p43. PMID- 42949451 OWN - NLM STAT- Publisher LR - 20261028 IS - 1724-6016 (Electronic) IS - 1120-6721 (Linking) DP - 2026 Oct 28 TI - Efficacy and safety of cylindrical annular refractive element spectacle lenses for myopia in children: A meta-analysis. PG - 11206721261502948 LID - 10.1177/11206721261502948 [doi] AB - BackgroundMyopia is an increasing global public health concern, particularly in East Asia. Cylindrical Annular Refractive Element (CARE) spectacle lenses aim to slow myopia progression by inducing peripheral myopic defocus. This meta-analysis evaluates the efficacy and safety of CARE lenses versus single-vision spectacle lenses in children.MethodsWe conducted a systematic search for randomized controlled trials comparing CARE spectacle lenses to single-vision lenses in children with myopia. Primary outcomes were mean change in spherical equivalent refraction and axial length. All analyses were performed using R software. Pooled mean differences and risk ratios with 95% confidence intervals were calculated using a random-effects model.ResultsAt 12 months, CARE lenses significantly reduced myopia progression compared to single-vision lenses. The treatment effect increased with longer follow-up, with one study reporting sustained benefit at 24 months. CARE lenses were associated with a significantly lower risk of fast progression. No significant differences were observed between groups in visual acuity change or discontinuation rates.ConclusionCARE spectacle lenses may be a well-tolerated, non-invasive option for slowing myopia progression in children, with modest benefits observed over one to two years. Larger, longer-term RCTs in diverse populations are needed to confirm these findings and establish sustained efficacy. FAU - Almutawtah, Sara AU - Almutawtah S AD - Department of Ophthalmology, Kuwait Institute for Medical Specialization: Kuwait City, Safat, Kuwait. RINGGOLD: 60599 FAU - Ahmad, Shahad T AU - Ahmad ST AD - Department of Ophthalmology, Kuwait Institute for Medical Specialization: Kuwait City, Safat, Kuwait. RINGGOLD: 60599 FAU - Alasoosi, Rashed A AU - Alasoosi RA AUID- ORCID: 0009-0009-6807-0906 AD - Department of Ophthalmology, Kuwait Institute for Medical Specialization: Kuwait City, Safat, Kuwait. RINGGOLD: 60599 FAU - Alhumaidi, Abdulwahab AU - Alhumaidi A AD - School of Medicine, Royal College of Surgeons In Ireland, Dublin, Ireland. RINGGOLD: 8863 FAU - Almutairi, Omar A AU - Almutairi OA AD - School of Medicine, Royal College of Surgeons In Ireland, Dublin, Ireland. RINGGOLD: 8863 FAU - Alzoabi, Abdullah AU - Alzoabi A AD - School of Medicine, Royal College of Surgeons In Ireland, Dublin, Ireland. RINGGOLD: 8863 FAU - Alrashed, Abdullah J AU - Alrashed AJ AD - College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Kingdom of Bahrain. RINGGOLD: 108541 FAU - Alqussair, Farah R AU - Alqussair FR AUID- ORCID: 0009-0003-0859-5299 AD - College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Kingdom of Bahrain. RINGGOLD: 108541 FAU - Mohammad, Fahad AU - Mohammad F AD - School of Medicine, Royal College of Surgeons In Ireland, Dublin, Ireland. RINGGOLD: 8863 FAU - Alharran, Abdullah M AU - Alharran AM AUID- ORCID: 0009-0003-2337-6588 AD - College of Medicine and Medical Sciences, Arabian Gulf University, Manama, Kingdom of Bahrain. RINGGOLD: 108541 LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Eur J Ophthalmol JT - European journal of ophthalmology JID - 9110772 SB - IM OTO - NOTNLM OT - CARE lenses OT - Cylindrical Annular Refractive Elements OT - Myopia control OT - axial length OT - children OT - meta-analysis OT - spectacle lenses EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 09:43 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:43 [entrez] AID - 10.1177/11206721261502948 [doi] PST - aheadofprint SO - Eur J Ophthalmol. 2026 Oct 28:11206721261502948. doi: 10.1177/11206721261502948. PMID- 42949450 OWN - NLM STAT- Publisher LR - 20261028 IS - 1550-5154 (Electronic) IS - 1063-8628 (Linking) DP - 2026 Oct 28 TI - Bioinformatics for Healthcare Quality Management: Clinical Outcomes, Implementation Barriers, and Care Improvement. LID - 10.1097/QMH.0000000000000600 [doi] AB - BACKGROUND AND OBJECTIVES: The bioinformatics-enabled workflows as quality-management tools have become central to modern healthcare, enabling genomic sequencing, transcriptomics, proteomics, and multiomics integration to support clinical decision-making. Despite expanding adoption, synthesized evidence on measurable clinical value and organizational, workforce, interoperability, governance, and equity barriers remains limited. To synthesize evidence on the contribution of bioinformatics-enabled workflows to healthcare quality improvement, care-process performance, clinical decision-making, patient outcomes, and implementation challenges from 2015 to 2025. METHODS: Following Preferred Reporting Items for Systematic Reviews and Meta-Analyses 2020 guidelines, we searched PubMed/MEDLINE, Scopus, Web of Science, Embase, and IEEE Xplore. We included studies from real-world healthcare or public health settings where bioinformatics supported diagnostic quality, timeliness, treatment appropriateness, patient safety, and workflow efficiency, or operational decision-making, reporting quantitative outcomes including diagnostic yield, time-to-diagnosis, treatment modification, or economic impact. RESULTS: Twenty studies met the selection criteria, spanning precision oncology, pharmacogenomics, infectious disease surveillance, clinical genomics and rare disease diagnosis, and multiomics biomarker translation. Across these domains, bioinformatics-enabled workflows improved diagnostic yield (25%-40% in rare disease genomics) and diagnostic accuracy, and shortened time-to-diagnosis, from 4-6 weeks to as little as 3 days in acute pediatric care and from weeks to under 24 hours for pathogen genomic surveillance. Workflows also supported treatment modification, with matched or altered therapy in 33%-37% of oncology cases, and improved pathogen detection and antimicrobial-resistance monitoring. Economic evidence, although still limited, was favorable: first-line exome sequencing was cost-saving, with average reductions of approximately US$9000 per patient in rare disease diagnosis. CONCLUSIONS: Although precision medicine relies heavily on bioinformatics, improvements to infrastructure, consistent governance, and capacity building for clinical staff are essential to ensure its scalable and equitable implementation. CI - Copyright (c) 2026 Wolters Kluwer Health, LLC. All rights reserved. FAU - Salam, Javed Us AU - Salam JU AD - Author Affiliation(s): Department of Bioscience, Comsats University, Islamabad, Pakistan (Mr Salam); Department of Precision Medicine, School of Medicine (Ms Yousaf, Dr Mir, and Dr Lee), Department of Metabiohealth, Institute for Cross-disciplinary Studies (Dr Lee), Department of Artificial Intelligence (Dr Lee), Personalized Cancer Immunotherapy Research Center, Sungkyunkwan University School of Medicine, Suwon, South Korea (Dr Lee); and Department of Family Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Jongno-gu Seoul, South Korea (Dr Lee). FAU - Yousaf, Maha AU - Yousaf M FAU - Mir, Bilal Ahmad AU - Mir BA FAU - Lee, Seung Won AU - Lee SW LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Qual Manag Health Care JT - Quality management in health care JID - 9306156 SB - IM OTO - NOTNLM OT - bioinformatics OT - clinical genomics OT - healthcare informatics OT - implementation barriers OT - precision medicine COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 09:43 PHST- 2026/05/31 00:00 [received] PHST- 2026/08/04 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:43 [entrez] AID - 00019514-990000000-00204 [pii] AID - 10.1097/QMH.0000000000000600 [doi] PST - aheadofprint SO - Qual Manag Health Care. 2026 Oct 28. doi: 10.1097/QMH.0000000000000600. PMID- 42949449 OWN - NLM STAT- Publisher LR - 20261028 IS - 1727-9445 (Electronic) IS - 1608-5906 (Linking) DP - 2026 Oct 28 TI - From integrity to impact: Strengthening governance in HIV/AIDS NGOS through transparency and accountability. PG - 1-11 LID - 10.2989/16085906.2026.2742897 [doi] AB - BACKGROUND: Weak governance in HIV/AIDS non-governmental organisations (NGOs) can undermine service delivery, erode donor confidence, and weaken community trust. Transparency and accountability are promoted as safeguards of integrity, yet their implementation often exposes tensions between donor oversight, organisational realities, and community expectations. AIM: This study examines how governance practices shape legitimacy and service delivery effectiveness in HIV/ AIDS NGOs in Gauteng Province, South Africa. METHODS: A qualitative design was employed, drawing on insights from NGO leaders engaged in donor relations and accountability processes. Twenty-eight purposively selected leaders from five HIV/AIDS NGOs participated in in-depth interviews. Data were thematically analysed to identify governance practices, accountability process contradictions, and perceived impacts on legitimacy and service delivery. RESULTS: Three themes emerged. First, negotiating donor demands and organisational realities: transparency mechanisms reassured funders but imposed administrative burdens that diverted staff time from service delivery. Second, community trust as contested legitimacy: participatory mechanisms such as social audits and monitoring committees were valued but often constrained by local hierarchies and resource limitations, risking symbolic rather than substantive accountability. Third, integrity in service delivery under pressure: ethical safeguards such as transparent procurement and confidentiality protections strengthened program reliability, but their absence led to resource misallocation and weakened trust. Across themes, participants highlighted contradictions between symbolic compliance and substantive accountability, as well as between donor oversight and community expectations. CONCLUSIONS: Transparency alone is insufficient to sustain legitimacy. Governance reforms are most effective when transparency, accountability, and integrity systems operate as interconnected components. Strengthening both donor oversight and community-based participatory mechanisms is essential to reinforcing trust, safeguarding resources, and ensuring equitable HIV/AIDS service delivery. FAU - Mpofu, Limkile AU - Mpofu L AUID- ORCID: 0000-0002-9505-5279 AD - College of Human Sciences, Department of Psychology, University of South Africa, Pretoria, South Africa. FAU - Govender, Kaymarlin AU - Govender K AUID- ORCID: 0000-0002-9586-1510 AD - Health Economics and HIV and AIDS Research Division (HEARD), University of KwaZulu-Natal, Durban, South Africa. LA - eng PT - Journal Article DEP - 20261028 PL - South Africa TA - Afr J AIDS Res JT - African journal of AIDS research : AJAR JID - 101146510 SB - IM OTO - NOTNLM OT - accountability OT - donor confidence OT - governance reforms OT - service delivery effectiveness OT - transparency EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 09:43 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:43 [entrez] AID - 10.2989/16085906.2026.2742897 [doi] PST - aheadofprint SO - Afr J AIDS Res. 2026 Oct 28:1-11. doi: 10.2989/16085906.2026.2742897. PMID- 42949439 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1939-1676 (Electronic) IS - 0891-6640 (Print) IS - 0891-6640 (Linking) VI - 40 IP - 5 DP - 2026 Sep 1 TI - Effects of a gluten-free hydrolyzed diet in dogs with paroxysmal dyskinesia: a prospective cohort study. LID - 10.1093/jvimsj/aalag255 [doi] LID - aalag255 AB - BACKGROUND: Paroxysmal dyskinesia (PD) is an episodic movement disorder in dogs. Gluten-sensitive etiology has been suggested, but prospective studies in different breeds are lacking. HYPOTHESIS/OBJECTIVES: Investigate whether a gluten-free hydrolyzed diet (GFHD) decreases PD episode frequency and gastrointestinal (GI) signs, and evaluate associations with serum anti-gliadin peptide immunoglobulin G (AGA IgG) and anti-transglutaminase-2 immunoglobulin A (TG-2 IgA) titers. ANIMALS: Twenty-three client-owned dogs with PD. METHODS: Prospective cohort study with 3 time points: baseline (T0), 90 days (T1), and 180 days (T2). Dogs were fed a GFHD. Episode frequency, GI signs and AGA IgG and TG-2 IgA titers were assessed at each time point. Response to GFHD was classified as excellent (episode disappearance), good (>/=50% decrease), fairly good (<50% decrease), or poor (no change or increase). RESULTS: Median number of episodes decreased from 6 (range, 2-60) at T0 to 2 (range, 0-10) at T2 (P = .0003). At T2, 17/23 dogs were responders (5 excellent, 12 good). Gastrointestinal signs were present in 15/23 dogs at T0 and in 6/23 dogs at T2, with significant decreases in vomiting and diarrhea (P = .01). No significant correlation was found between episode frequency and AGA IgG or TG-2 IgA titers at any time point (P > .1). Antibody results did not significantly change between T0 and T2. CONCLUSIONS AND CLINICAL IMPORTANCE: The GFHD was associated with a significant decrease in PD frequency and GI signs in most dogs. The AGA IgG and TG-2 IgA titers were not predictive of dietary response, suggesting limited value as biomarkers. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the American College of Veterinary Internal Medicine. FAU - Okonji, Samuel AU - Okonji S AD - Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy. AD - Veterinaria Corlazzoli, Rome, Italy. FAU - Del Baldo, Francesca AU - Del Baldo F AD - Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy. FAU - Garuti, Elisa AU - Garuti E AD - Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy. FAU - Vecchiato, Carla G AU - Vecchiato CG AD - Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy. FAU - Di Carlo, Alessandro AU - Di Carlo A AD - Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy. FAU - Costantini, Gaia AU - Costantini G AD - Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy. FAU - Cauduro, Alberto AU - Cauduro A AD - Neurovet, Legnano, Milan, Italy. FAU - Di Paola, Andrea AU - Di Paola A AD - Neurovet, Legnano, Milan, Italy. FAU - Falzone, Cristian AU - Falzone C AD - Clinica Veterinaria Pedrani, Diagnostica Piccoli Animali Srl, Zugliano, Italy. FAU - Gallucci, Antonella AU - Gallucci A AD - Veterinary Neurological Center ;La Fenice,' Selargius, Italy. FAU - Tartari, Ilaria AU - Tartari I AD - Veterinary Neurological Center ;La Fenice,' Selargius, Italy. FAU - Baroni, Massimo AU - Baroni M AD - Valdinievole Veterinary Clinic, Monsummano Terme, Italy. FAU - Poli, Federica AU - Poli F AD - Valdinievole Veterinary Clinic, Monsummano Terme, Italy. FAU - Bianchi, Ezio AU - Bianchi E AD - Department of Veterinary Medical Sciences, University of Parma, Parma, Italy. FAU - Buffagni, Valentina AU - Buffagni V AD - Department of Veterinary Medical Sciences, University of Parma, Parma, Italy. FAU - Valentini, Alessia AU - Valentini A AD - AniCura Clinica Veterinaria Arcella, Padova, Italy. FAU - Gagliardo, Teresa AU - Gagliardo T AD - PalermoVet Veterinary Diagnostic Center, Palermo, Italy. FAU - Gandini, Gualtiero AU - Gandini G AD - Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy. LA - eng GR - Nestle Purina PetCare Europe/ PT - Journal Article PL - England TA - J Vet Intern Med JT - Journal of veterinary internal medicine JID - 8708660 RN - 0 (Immunoglobulin A) RN - 0 (Immunoglobulin G) RN - 9007-90-3 (Gliadin) RN - EC 2.3.2.13 (Transglutaminases) RN - EC 2.3.2.13 (Protein Glutamine gamma Glutamyltransferase 2) RN - EC 3.6.1.- (GTP-Binding Proteins) SB - IM MH - Animals MH - Dogs MH - *Dog Diseases/diet therapy MH - Female MH - Prospective Studies MH - Immunoglobulin A/blood MH - Male MH - *Diet, Gluten-Free/veterinary MH - *Chorea/veterinary/diet therapy MH - Immunoglobulin G/blood MH - Gliadin/immunology MH - Transglutaminases/immunology MH - Protein Glutamine gamma Glutamyltransferase 2 MH - GTP-Binding Proteins/immunology MH - Cohort Studies PMC - PMC13714782 OTO - NOTNLM OT - antibodies OT - canine OT - dystonia OT - involuntary movement OT - movement disorder OT - seizure COIS- Potential conflicts of interest related to industry funding were managed through independent study design, conduct, and analysis by the academic investigators. The sponsor had no role in case selection, data collection, outcome assessment, statistical analysis, or interpretation of results. To decrease bias, the study used a prospective multicenter design with predefined inclusion criteria, standardized diagnostic confirmation of paroxysmal dyskinesia based on neurologist review of video-recorded episodes, and objective outcome measures (episode frequency). Laboratory analyses were performed by an external accredited laboratory, and statistical methods were predefined in the study protocol. These measures are described in the "Materials and methods" section of the manuscript. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/28 CRDT- 2026/10/28 09:33 PHST- 2026/02/22 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:33 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 9003621 [pii] AID - aalag255 [pii] AID - 10.1093/jvimsj/aalag255 [doi] PST - ppublish SO - J Vet Intern Med. 2026 Sep 1;40(5):aalag255. doi: 10.1093/jvimsj/aalag255. PMID- 42949438 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1939-1676 (Electronic) IS - 0891-6640 (Print) IS - 0891-6640 (Linking) VI - 40 IP - 5 DP - 2026 Sep 1 TI - Outcomes of a 42-day course of molnupiravir in cats with feline infectious peritonitis: a prospective descriptive study. LID - 10.1093/jvimsj/aalag268 [doi] LID - aalag268 AB - BACKGROUND: Antiviral therapy for cats with feline infectious peritonitis (FIP) has traditionally been administered for 84 days, although the optimal duration of molnupiravir treatment remains unknown. HYPOTHESIS/OBJECTIVES: To evaluate the efficacy of a 42-day course of molnupiravir, orally (PO), for naturally occurring FIP in cats. ANIMALS: Nineteen client-owned cats with newly diagnosed FIP. METHODS: Prospective pilot study. Cats received molnupiravir, PO, q12h for 42 days at a median dosage of 11.5 mg/kg (IQR, 10.2-12.5 mg/kg). Cats with ocular or neurological involvement were excluded. Clinical assessments were performed during treatment and for 6 months after treatment cessation. RESULTS: Nineteen cats were enrolled. Two cats failed to complete treatment because of death or persistent clinical signs. Fourteen of 19 cats (74%; 95% CI, 49-91) achieved sustained remission on an intention-to-treat basis. Each non-completer failed on day 5 or 10, respectively, within a window common to 42- and 84-day protocols, and therefore do not inform the question of treatment duration; among the 17 cats reaching day 42, 14 (82%; 95% CI, 57-96) remained in remission for at least 6 months. Three cats relapsed after treatment cessation; all responded to retreatment with molnupiravir at the same dosage extended to 84 days. CONCLUSIONS AND CLINICAL IMPORTANCE: This prospective study suggests that a shortened course of molnupiravir is feasible in selected cats with non-ocular, non-neurological FIP. ABSTRACT (JAPANESE): : (FIP) にするウイルスは 84 されてきたが、モルヌピラビルのなはらかではない。 : FIP のにするモルヌピラビル 42 のをすること。 : たに FIP とされた19。 : きパイロット。モルヌピラビルを 12 ごとに 42 した。のは 11.5 mg/kg (、 10.2-12.5 mg/kg) であった。またはをするはした。および 6 か、をった。 : 19 をした。 2 はまたはのによりをできなかった。 Intention-to-treat では 19 14 (74 %； 95% 、 49-91) がをした。の 2 はそれぞれ5および10にとなり、いずれも 42 および 84 のプロトコルにするであったため、のにはしなかった。 42 までをした 17 のうち 14 (82% ； 95% 、 57-96) はなくとも 6 かをした。 3 はにしたが、がのモルヌピラビルによる 84 のにした。 および: きパイロットのから、およびをわない FIP のでは、モルヌピラビルのがであるがされた。. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the American College of Veterinary Internal Medicine. FAU - Hishinuma, Kenta AU - Hishinuma K AUID- ORCID: 0009-0006-9913-4413 AD - Veterinary Specialists Emergency Center, Saitama 333-0823, Japan. FAU - Inoue, Akiko AU - Inoue A AUID- ORCID: 0009-0008-7870-109X AD - Veterinary Specialists Emergency Center, Saitama 333-0823, Japan. FAU - Fukushima, Kenjiro AU - Fukushima K AUID- ORCID: 0000-0003-4271-8267 AD - Veterinary Specialists Emergency Center, Saitama 333-0823, Japan. FAU - Sato, Masahiko AU - Sato M AUID- ORCID: 0000-0002-5611-4483 AD - Veterinary Specialists Emergency Center, Saitama 333-0823, Japan. LA - eng PT - Journal Article PL - England TA - J Vet Intern Med JT - Journal of veterinary internal medicine JID - 8708660 RN - 0 (Antiviral Agents) SB - IM MH - Animals MH - Cats MH - *Feline Infectious Peritonitis/drug therapy MH - Female MH - Prospective Studies MH - Male MH - *Antiviral Agents/therapeutic use/administration & dosage MH - Treatment Outcome MH - Pilot Projects PMC - PMC13714784 OAB - Feline infectious peritonitis (FIP) is a serious disease in cats caused by feline coronavirus. Antiviral treatment is commonly given for 84 days, but the best treatment duration for molnupiravir is unknown. In this prospective pilot study, 19 cats without eye or neurological involvement received molnupiravir by mouth for 42 days. Seventeen cats completed the 42-day course. Fourteen of the 19 enrolled cats remained in remission for at least 6 months after treatment. Three cats relapsed after completing treatment. All 3 responded when molnupiravir was restarted at the same dose and continued for approximately 84 days. Two cats did not complete the initial treatment course because of death or persistent clinical signs early in treatment. These findings suggest that a 42-day course of molnupiravir can be feasible for selected cats with FIP, although this study was small and did not directly compare 42-day and 84-day treatment courses. OABL- eng OTO - NOTNLM OT - antiviral therapy OT - feline infectious peritonitis OT - molnupiravir OT - nucleoside analogue OT - treatment duration COIS- Authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/28 CRDT- 2026/10/28 09:33 PHST- 2026/02/05 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:33 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 9003617 [pii] AID - aalag268 [pii] AID - 10.1093/jvimsj/aalag268 [doi] PST - ppublish SO - J Vet Intern Med. 2026 Sep 1;40(5):aalag268. doi: 10.1093/jvimsj/aalag268. PMID- 42949435 OWN - NLM STAT- Publisher LR - 20261028 IS - 1533-4023 (Electronic) IS - 0160-2446 (Linking) DP - 2026 Oct 28 TI - Prognostic Value of Non-Invasive Hepatic Fibrosis Scores Across Acute Coronary Syndrome Subtypes. LID - 10.1097/FJC.0000000000001896 [doi] AB - Hepatic fibrosis indices have emerged as potential prognostic markers in cardiovascular disease. This observational study included 760 consecutive patients with acute coronary syndrome (STEMI, NSTEMI, and unstable angina pectoris) admitted between March 2020 and May 2024 to evaluate the distribution of APRI, FIB-4, FIB-5, and FIB-6 across ACS subtypes and their predictive value for in-hospital mortality and cardiogenic shock. Fibrosis indices were calculated from admission laboratory values, and multivariate logistic regression and ROC curve analyses were performed. STEMI patients had significantly higher APRI (0.95+/-1.43 vs. 0.41+/-0.71 vs. 0.20+/-0.11), FIB-4 (3.68+/-4.03 vs. 1.98+/-2.17 vs. 1.17+/-0.52), and FIB-5 (5.16+/-1.08 vs. 4.66+/-0.84 vs. 4.38+/-0.43) than NSTEMI and unstable angina patients (all p<0.001). In-hospital mortality and cardiogenic shock occurred in 3.8% and 3.2% of patients, respectively. Cardiogenic shock, reduced left ventricular ejection fraction, and lower serum albumin independently predicted mortality, whereas older age independently predicted cardiogenic shock. None of the hepatic fibrosis indices independently predicted either outcome, and their discriminative performance was limited (AUC 0.526-0.632 for mortality and 0.570-0.662 for cardiogenic shock). These findings indicate that although hepatic fibrosis scores are elevated in STEMI, they primarily reflect the severity of acute myocardial injury rather than providing incremental prognostic value beyond established clinical predictors in patients with acute coronary syndrome. CI - Copyright (c) 2026 Wolters Kluwer Health, LLC. All rights reserved. FAU - Kaya, Ahmet Ferhat AU - Kaya AF AD - Department of Cardiology, Van Regional Education and Research Hospital, Van, Turkey. FAU - Polat, Fuat AU - Polat F AUID- ORCID: 0000-0002-6414-3743 AD - Department of Cardiology, Dr. Siyami Ersek Thoracic and Cardiovascular Surgery Education Research Hospital, Istanbul, Turkey. FAU - Ayhan, Gorkem AU - Ayhan G AD - Department of Cardiology, Van Regional Education and Research Hospital, Van, Turkey. FAU - Yetmis, Furkan AU - Yetmis F AD - Department of Cardiology, Mus State Hospital, Mus, Turkey. FAU - Ozbek, Emrah AU - Ozbek E AD - Department of Cardiology, Van Regional Education and Research Hospital, Van, Turkey. FAU - Can, Veysi AU - Can V AD - Department of Cardiology, Van Regional Education and Research Hospital, Van, Turkey. FAU - Kumet, Omer AU - Kumet O AD - Department of Cardiology, Van Regional Education and Research Hospital, Van, Turkey. AD - Saglik Bilimleri Universitesi, Istanbul, Turkey. FAU - Aktan, Adem AU - Aktan A AD - Department of Cardiology, Mardin Artuklu University, Mardin, Turkey. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Cardiovasc Pharmacol JT - Journal of cardiovascular pharmacology JID - 7902492 SB - IM OTO - NOTNLM OT - acute coronary syndrome OT - cardiogenic shock OT - hepatic fibrosis indices OT - in-hospital mortality OT - prognostic markers COIS- Conflict of Interest: The authors declare no conflicts of interest, financial or otherwise, that could influence the objectivity of the research presented in this manuscript. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 09:33 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:33 [entrez] AID - 00005344-990000000-00626 [pii] AID - 10.1097/FJC.0000000000001896 [doi] PST - aheadofprint SO - J Cardiovasc Pharmacol. 2026 Oct 28. doi: 10.1097/FJC.0000000000001896. PMID- 42949430 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1471-8391 (Electronic) IS - 0007-1420 (Linking) VI - 160 IP - 1 DP - 2026 Oct 5 TI - Psychological factors as predictors of compliance and outcome in non-operative management of Achilles tendon rupture: a systematic review. LID - ldag041 [pii] LID - 10.1093/bmb/ldag041 [doi] AB - INTRODUCTION: Non-operative management of acute Achilles tendon rupture (ATR) with early functional rehabilitation produces functional outcomes equivalent to surgery, but its effectiveness depends critically on patient compliance with bracing, weight-bearing and physiotherapy. Psychological factors, including motivation, self-efficacy, kinesiophobia, and social support, are plausible determinants of compliance and outcome, yet have received minimal systematic attention. SOURCES OF DATA: Seven databases were searched from inception to June 2026 without language restriction. Studies of adults with ATR managed non-operatively, with any psychological assessment reporting compliance, re-rupture rate, Achilles Tendon Total Rupture Score (ATRS) or return to sport, were eligible. Six studies met the inclusion criteria (797 non-operative patients in total), and two further non-operative randomized controlled trials were used for functional benchmarking only. Data were synthesized narratively (SWiM framework). AREAS OF AGREEMENT: Non-operative management with early functional rehabilitation yields ATRS scores of 72-86 at 6-12 months, comparable to operative cohorts, with re-rupture rates of 5%-12% under functional bracing. Treatment success depends on patient compliance, and functional deficits persist for years irrespective of the management modality. AREAS OF CONTROVERSY: Surgery continues to carry a lower re-rupture incidence than conservative management, although the absolute difference has narrowed with early functional rehabilitation. Whether pre-treatment psychological profiling should inform the choice between operative and non-operative management remains unvalidated, and no included study reported compliance as a formal outcome or its association with any psychological factor. GROWING POINTS: Kinesiophobia, anxiety, and depression are present throughout the first year of non-operative rehabilitation, and kinesiophobia scores above the clinical cut-off were observed in most participants of the only dedicated non-operative cohort. Kinesiophobia and readiness-to-return scores do not appear to differ between operatively and non-operatively managed patients, supporting a common psychological assessment framework. Qualitative evidence identifies motivation and social support as key facilitators of recovery. AREAS TIMELY FOR DEVELOPING RESEARCH: Future non-operative ATR randomized trials should incorporate the I-PRRS, TSK-11, and a validated motivation measure as pre-specified secondary outcomes, together with guideline-based compliance definitions. A prospective cohort study of baseline psychological profile as a predictor of compliance and re-rupture should be prioritized, followed by evaluation of simple psychological screening within consent and treatment-selection pathways. CI - (c) The Author(s) 2026. Published by Oxford University Press. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Maffulli, Nicola AU - Maffulli N AUID- ORCID: 0000-0002-5327-3702 AD - Department of Trauma and Orthopaedic Surgery, Faculty of Medicine and Psychology, University La Sapienza, Piazzale Aldo Moro, 5, 00185, Rome, Italy. AD - Centre for Sports and Exercise Medicine, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, 275 Bancroft Road, London E14 9DG, United Kingdom. AD - School of Pharmacy and Bioengineering, Keele University School of Medicine, Stoke on Trent, Staffordshire, ST4 6QG, United Kingdom. FAU - Spiezia, Filippo AU - Spiezia F AUID- ORCID: 0000-0002-3710-7982 AD - Department of Health Sciences, University of Basilicata, Via dell'Ateneo Lucano, 10, 85100, Potenza, Italy. AD - Department of Orthopaedics and Trauma Surgery, San Carlo Hospital, Via Potito Petrone, SNC, Potenza 85100, Basilicata, Italy. LA - eng PT - Journal Article PT - Systematic Review PL - England TA - Br Med Bull JT - British medical bulletin JID - 0376542 SB - IM MH - Humans MH - *Achilles Tendon/injuries MH - Rupture/psychology/therapy/rehabilitation MH - *Patient Compliance/psychology MH - Kinesiophobia MH - *Tendon Injuries/psychology/therapy/rehabilitation MH - Treatment Outcome MH - Social Support MH - Self Efficacy MH - *Conservative Treatment/psychology/methods MH - Physical Therapy Modalities MH - Motivation MH - Braces OTO - NOTNLM OT - ATRS OT - Achilles tendon rupture OT - compliance OT - conservative treatment OT - functional brace OT - kinesiophobia OT - motivation OT - non-operative management OT - psychological predictors OT - rehabilitation EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 09:33 PHST- 2026/07/21 00:00 [received] PHST- 2026/08/20 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:33 [entrez] AID - 9003616 [pii] AID - 10.1093/bmb/ldag041 [doi] PST - ppublish SO - Br Med Bull. 2026 Oct 5;160(1):ldag041. doi: 10.1093/bmb/ldag041. PMID- 42949429 OWN - NLM STAT- Publisher LR - 20261028 IS - 1537-2995 (Electronic) IS - 0041-1132 (Linking) DP - 2026 Oct 28 TI - Shelf-life of cord blood red cell concentrates: Comparison of two additive solutions. LID - 10.1111/trf.70468 [doi] AB - BACKGROUND: Cord blood-derived red blood cell concentrates (CB-RBCs) may provide a physiologically tailored transfusion product for preterm neonates, but their limited shelf life remains a major barrier to clinical implementation. We longitudinally characterized CB-RBC storage over 28 days and evaluated the effect of additive solution composition in DEHP-free storage bags. STUDY DESIGN AND METHODS: Thirty-eight CB-RBC units were stored in either PAGGSM (n = 18) or SAGM (n = 20) at 2-6 degrees C for 28 days. Hematological, biochemical, metabolic, and flow-cytometric parameters were assessed at baseline and on days 7, 14, 21, and 28. Longitudinal changes were evaluated using linear mixed-effects models. RESULTS: All units met predefined quality specifications at baseline. Hemolysis increased over time but was unaffected by additive solution, with approximately 90% of units remaining below the 0.8% threshold at day 28. PAGGSM and SAGM showed distinct electrolyte profiles, with lower sodium and chloride and higher inorganic phosphate concentrations in PAGGSM. Potassium and lactate increased in both groups, but their accumulation was significantly lower with PAGGSM, particularly during the second half of storage. Changes in red cell indices also differed between solutions, with a smaller increase in MCV and better maintenance of MCHC with PAGGSM. ATP progressively declined, while a distinct CFSE-high RBC population emerged over storage. CONCLUSIONS: CB-RBC maintained acceptable in vitro quality over 28 days of storage in DEHP-free bags. PAGGSM was associated with distinct temporal patterns in selected metabolic, ionic, and red cell indices, warranting further investigation as an alternative additive solution for CB-RBCs. CI - (c) 2026 AABB. FAU - Pellegrino, Claudio AU - Pellegrino C AUID- ORCID: 0000-0002-1614-5576 AD - Fondazione Policlinico A. Gemelli IRCCS, Rome, Italy. AD - Universita Cattolica del Sacro Cuore, Rome, Italy. FAU - Degni, Giorgia AU - Degni G AD - Fondazione Policlinico A. Gemelli IRCCS, Rome, Italy. AD - Universita Cattolica del Sacro Cuore, Rome, Italy. FAU - Pilla, Milena AU - Pilla M AD - Universita Cattolica del Sacro Cuore, Rome, Italy. FAU - Fabbri, Marco AU - Fabbri M AD - Transfusion Medicine, Azienda Ospedaliero Universitaria Pisana, Pisa, Italy. FAU - Gabbriellini, Sabrina AU - Gabbriellini S AD - Transfusion Medicine, Azienda Ospedaliero Universitaria Pisana, Pisa, Italy. FAU - Baroni, Silvia AU - Baroni S AD - Fondazione Policlinico A. Gemelli IRCCS, Rome, Italy. AD - Universita Cattolica del Sacro Cuore, Rome, Italy. FAU - Urbani, Andrea AU - Urbani A AD - Fondazione Policlinico A. Gemelli IRCCS, Rome, Italy. AD - Universita Cattolica del Sacro Cuore, Rome, Italy. FAU - Teofili, Luciana AU - Teofili L AUID- ORCID: 0000-0002-7214-1561 AD - Universita Cattolica del Sacro Cuore, Rome, Italy. AD - Centro Nazionale Sangue, Rome, Italy. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Transfusion JT - Transfusion JID - 0417360 SB - IM OTO - NOTNLM OT - additive solutions OT - blood storage OT - cord blood OT - neonatal transfusion OT - red blood cells OT - storage lesion OT - transfusion medicine EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 09:33 PHST- 2026/10/13 00:00 [revised] PHST- 2026/09/10 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:33 [entrez] AID - 10.1111/trf.70468 [doi] PST - aheadofprint SO - Transfusion. 2026 Oct 28. doi: 10.1111/trf.70468. PMID- 42949427 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1555-2101 (Electronic) IS - 0160-6689 (Linking) VI - 87 IP - 4 DP - 2026 Oct 26 TI - Lithium in the Prevention of Postpartum Recurrence of Mood Disorders: A Systematic Review and Meta-Analysis. LID - 26r16567 [pii] LID - 10.4088/JCP.26r16567 [doi] AB - Background: Women with bipolar disorder are at increased risk for relapse/recurrence during the postpartum period. This risk decreases with prophylactic pharmacologic treatment, compared to those who remain medication-free. Lithium use in preventing relapse has been studied extensively in individuals with bipolar disorder, though its specific use in the postpartum has not received as much attention. Objective: The aim of this systematic review (CRD420261357096) was to estimate risk of postpartum relapse, specifically in women with bipolar disorder who received lithium prophylactically versus those who discontinued it during pregnancy. Study Selection: Following PRISMA guidelines, reviewers screened 45 studies focused on women with bipolar I or II disorders from searches of PubMed, PsycINFO, and Cochrane databases conducted on April 2, 2026, that were written in English. Data Extraction: Two independent observers extracted data using a data extraction form created on Covidence. Random-effects meta-analysis of recurrence proportions, between-study variance, heterogeneity, and leave-one-out sensitivity analyses were conducted. Results: Eight studies (554 women) providing information on the prophylactic effect of lithium during the postpartum period, and 2 studies evaluating the impact of lithium discontinuation on postpartum relapse, were identified. The pooled recurrence proportion was 0.21 (95% CI 0.15-0.29) in lithium-treated patients and 0.41 (95% CI 0.33-0.50) in lithium-untreated patients. The most common timing of prophylaxis initiation was during pregnancy (51.9%). Studies examining the impact of lithium discontinuation provided indirect evidence of its effectiveness in preventing postpartum recurrence. Conclusion: Findings from our meta-analysis suggest that lithium is effective in preventing postpartum recurrence of mood disorders; however, caution is warranted when interpreting study results because of confounding by indication and limited literature. CI - (c) Copyright 2026 Physicians Postgraduate Press, Inc. FAU - Sharma, Verinder AU - Sharma V AD - Department of Psychiatry, Western University, London, Ontario, Canada. AD - Department of Obstetrics & Gynecology, Western University, London, Ontario, Canada. AD - Parkwood Institute Mental Health, St. Joseph's Health Care, London, Ontario, Canada. AD - Corresponding Author: Verinder Sharma, MB, BS, FRCP(C), Parkwood Institute Mental Health, St. Joseph's Health Care, 550 Wellington Road, London, ON, N6C 0A7, Canada (vsharma@uwo.ca). FAU - Dietrich, Emily L AU - Dietrich EL AD - Lawson Research Institute, St. Joseph's Health Care, London, Ontario, Canada. FAU - Cha, Boseok AU - Cha B AD - Department of Psychiatry, Gyeongsang National University College of Medicine, Jinju, Republic of Korea. FAU - Gonzalez-Torres, Christina AU - Gonzalez-Torres C AD - Department of Psychiatry, Western University, London, Ontario, Canada. AD - Parkwood Institute Mental Health, St. Joseph's Health Care, London, Ontario, Canada. FAU - Mazmanian, Dwight AU - Mazmanian D AD - Department of Psychology, Lakehead University, Thunder Bay, Ontario, Canada. LA - eng PT - Journal Article PT - Meta-Analysis PT - Systematic Review DEP - 20261026 PL - United States TA - J Clin Psychiatry JT - The Journal of clinical psychiatry JID - 7801243 RN - 0 (Lithium Compounds) RN - 0 (Antimanic Agents) SB - IM MH - Humans MH - Female MH - *Secondary Prevention/methods MH - *Bipolar Disorder/drug therapy/prevention & control MH - *Postpartum Period MH - *Lithium Compounds/therapeutic use MH - Pregnancy MH - *Antimanic Agents/therapeutic use MH - Recurrence COIS- Drs Sharma, Cha, Gonzalez-Torres, and Mazmanian and Ms Dietrich have no conflicts to disclose. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 09:33 PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:33 [entrez] AID - 26r16567 [pii] AID - 10.4088/JCP.26r16567 [doi] PST - epublish SO - J Clin Psychiatry. 2026 Oct 26;87(4):26r16567. doi: 10.4088/JCP.26r16567. PMID- 42949425 OWN - NLM STAT- Publisher LR - 20261028 IS - 2150-136X (Electronic) IS - 2150-1351 (Linking) DP - 2026 Oct 28 TI - Bronchoscopy in Pediatric Cardiac Surgery Patients: Airway Findings, Clinical Outcomes, and the Selective Role of Rigid Bronchoscopy. PG - 21501351261497290 LID - 10.1177/21501351261497290 [doi] AB - BackgroundThis study evaluated the indications, airway findings, therapeutic interventions, and clinical outcomes associated with bronchoscopy in pediatric cardiac surgery patients, including the selective role of rigid bronchoscopy.MethodsThis retrospective, single-center study included patients who underwent bronchoscopy between January 2019 and January 2025. Among 1840 surgical patients, 60 required bronchoscopy because of prolonged mechanical ventilation, extubation failure, difficulty weaning from ventilatory support, or suspected airway pathology.ResultsThe median age of the patients was 9 months, and the first bronchoscopy was performed at a median of 8.5 postoperative days (range, 5-24 days). Findings were normal in 32/60 patients (53.3%). The most common abnormalities were mucus plugging/secretion retention (20.0%, 12/60), blood clots (8.3%, 5/60), and tracheomalacia (8.3%, 5/60). Thirteen patients underwent 16 rigid bronchoscopic procedures. Bronchoscopy guided subsequent management, including diaphragmatic plication in four patients and surgery for bronchial compression in three. Among 17 patients with mucus plugging/secretion retention or blood clot, eight achieved successful extubation without tracheostomy. Tracheostomy was performed in 34/60 patients (56.7%), and in-hospital mortality in this group was 28.3% (17/60). Brief transient oxygen desaturation occurred occasionally, but no major procedure-related complications were observed.ConclusionsBronchoscopy provides clinically useful diagnostic and therapeutic information in selected pediatric cardiac surgery patients. Mucus plugging or retained secretions were the most common abnormal finding, while normal findings helped redirect evaluation toward alternative causes of ventilator dependence. Flexible bronchoscopy was adequate for most patients, whereas rigid bronchoscopy was reserved for selected cases of dense mucus plugs or organized blood clots that could not be adequately removed with flexible techniques. FAU - Okumus, Mustafa AU - Okumus M AUID- ORCID: 0000-0001-8705-5183 AD - Department of Pediatric Surgery, Gaziosmanpasa Hospital, Istanbul Yeni Yuzyil University, Istanbul, Turkiye. RINGGOLD: 469683 FAU - Suzan, Dilek AU - Suzan D AUID- ORCID: 0000-0002-1220-5826 AD - Department of Pediatric Cardiovascular Surgery, Gaziosmanpasa Hospital, Istanbul Yeni Yuzyil University, Istanbul, Turkiye. RINGGOLD: 469683 FAU - Yazicioglu, Volkan AU - Yazicioglu V AUID- ORCID: 0000-0001-5693-4978 AD - Department of Pediatric Cardiovascular Surgery, Gaziosmanpasa Hospital, Istanbul Yeni Yuzyil University, Istanbul, Turkiye. RINGGOLD: 469683 FAU - Balaban, Ismail AU - Balaban I AUID- ORCID: 0000-0001-9424-9760 AD - Department of Pediatric Cardiology, Gaziosmanpasa Hospital, Istanbul Yeni Yuzyil University, Istanbul, Turkiye. RINGGOLD: 469683 FAU - Kilercik, Hakan AU - Kilercik H AUID- ORCID: 0000-0003-1624-0124 AD - Department of Anesthesiology and Reanimation, Gaziosmanpasa Hospital, Istanbul Yeni Yuzyil University, Istanbul, Turkiye. RINGGOLD: 469683 FAU - Yildirim, Ozgur AU - Yildirim O AUID- ORCID: 0000-0002-6842-135X AD - Department of Pediatric Cardiovascular Surgery, Gaziosmanpasa Hospital, Istanbul Yeni Yuzyil University, Istanbul, Turkiye. RINGGOLD: 469683 LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - World J Pediatr Congenit Heart Surg JT - World journal for pediatric & congenital heart surgery JID - 101518415 SB - IM OTO - NOTNLM OT - Pediatric cardiac surgery OT - airway obstruction OT - bronchoscopy OT - mechanical ventilation OT - mucus plugging OT - rigid bronchoscopy EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 09:33 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:33 [entrez] AID - 10.1177/21501351261497290 [doi] PST - aheadofprint SO - World J Pediatr Congenit Heart Surg. 2026 Oct 28:21501351261497290. doi: 10.1177/21501351261497290. PMID- 42949423 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1502-3850 (Electronic) IS - 0001-6357 (Linking) VI - 85 DP - 2026 Oct 28 TI - Research progress on the effects of implant-supported overdentures on residual ridge remodelling in edentulous jaws: a narrative review. PG - 741-750 LID - 10.2340/aos.v85.47067 [doi] AB - Residual ridge resorption (RRR) following tooth loss is a continuous and irreversible process that limits the effectiveness of conventional complete dentures and compromises masticatory function and quality of life. Implant-supported overdentures (implant overdentures [IODs]) can reconstruct physiological stress distribution, regulate inflammatory molecular signalling and optimise bone remodelling through combined biological and mechanical pathways, thereby helping to slow RRR. This review systematically summarises the biological mechanisms of RRR, including inflammatory mediators, the receptor activator of nuclear factor-kappa B ligand/osteoprotegerin pathway and systemic factors; the mechanical mechanisms, including Wolff's law and stress distribution patterns; the protective effects of IODs on residual ridges; key determinants of their bone-preserving efficacy, including implant-, attachment-, occlusion- and patient-related factors; current controversies; and advances in digital applications. Regarding attachment systems, current evidence from randomised controlled trials, long-term cohort studies and meta-analyses indicates that ball, Locator and bar attachments demonstrate broadly comparable peri-implant marginal bone outcomes, although Locator attachments may be associated with lower posterior RRR over 10 years, whereas bar attachments may offer superior crestal bone stability in specific clinical scenarios. No single attachment system demonstrates a clinically meaningful, reproducible advantage in bone preservation, and selection should be individualised based on anatomical, functional and maintenance considerations. Current evidence remains limited by heterogeneous study designs, variable follow-up periods and an overreliance on finite element analyses, underscoring the need for standardised long-term clinical trials with harmonised outcome measures. Evidence from recent high-quality studies over the past 5 years is integrated to propose stratified clinical recommendations according to bone volume, systemic status and economic factors. Digital technologies, including computer-guided surgery, digital impressions and occlusal analysis, further enhance precision and predictability of bone-preserving outcomes. This review provides a theoretical basis and clinical reference for optimising rehabilitation protocols, preserving residual ridges over the long term and improving prosthetic prognosis in patients with edentulism. FAU - Fan, Nana AU - Fan N AD - Department of Prosthodontics, Wuxi Stomatology Hospital, Wuxi City, Jiangsu Province, China. FAU - Gong, Liqin AU - Gong L AD - Department of Prosthodontics, Wuxi Stomatology Hospital, Wuxi City, Jiangsu Province, China. FAU - Tang, Liqin AU - Tang L AD - Department of Prosthodontics, Wuxi Stomatology Hospital, Wuxi City, Jiangsu Province, China. FAU - Xue, Qiang AU - Xue Q AD - Department of Prosthodontics, Wuxi Stomatology Hospital, Wuxi City, Jiangsu Province, China. xueqiang75_Q@163.com. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - Sweden TA - Acta Odontol Scand JT - Acta odontologica Scandinavica JID - 0370344 SB - IM MH - Humans MH - *Denture, Overlay MH - *Bone Remodeling MH - *Jaw, Edentulous MH - *Dental Prosthesis, Implant-Supported EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 09:33 PHST- 2026/05/25 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:33 [entrez] AID - 10.2340/aos.v85.47067 [doi] PST - epublish SO - Acta Odontol Scand. 2026 Oct 28;85:741-750. doi: 10.2340/aos.v85.47067. PMID- 42949399 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1530-6992 (Electronic) IS - 1530-6984 (Linking) VI - 26 IP - 42 DP - 2026 Oct 28 TI - Ultrathin BiOx Charge-Transfer Doping for Cross-TMD Electronic Modulation and Homogeneous WSe2 Inverters. PG - 14675-14682 LID - 10.1021/acs.nanolett.6c03585 [doi] AB - Charge-transfer doping provides a nondestructive route to tune two-dimensional transition-metal dichalcogenides (TMDs), but selectively patterned n-type dopants effective across different TMD channels remain limited. Here, ultrathin bismuth oxide (BiOx) is used as an n-type charge-transfer doping and passivation layer for monolayer MoS2 and WSe2 transistors. In MoS2, 2 nm BiOx preserves the channel structure and yields a TLM-extracted effective contact resistance of 0.71 +/- 0.06 kOmega.mum at n2D = 1.8 x 1013 cm-2. Thicker BiOx strengthens n-type modulation but reduces gate controllability. The modulation remains stable after ambient storage and air annealing. BiOx also enhances electron transport in WSe2 and enables homogeneous WSe2 inverters with MoOx-doped p-FETs, achieving a gain of approximately 19 and a total normalized noise margin of 0.895 VDD at VDD = 2 V. These results establish BiOx as a cross-TMD n-type charge-transfer dopant for contact, threshold-voltage, and polarity engineering. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Hsu, Ching-Hao AU - Hsu CH AUID- ORCID: 0009-0007-2928-5555 AD - Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei106, Taiwan. FAU - Hsu, Yu-Wei AU - Hsu YW AUID- ORCID: 0009-0002-1510-5975 AD - Graduate School of Advanced Technology, National Taiwan University, Taipei106, Taiwan. FAU - Hsu, Ta-Wei AU - Hsu TW AD - Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei106, Taiwan. FAU - Lin, Yu-Tung AU - Lin YT AD - Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei106, Taiwan. FAU - Chiu, Li-Ya AU - Chiu LY AD - Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei106, Taiwan. FAU - Ni, I-Chih AU - Ni IC AD - Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei106, Taiwan. FAU - Wu, Chih-I AU - Wu CI AUID- ORCID: 0000-0003-3613-7511 AD - Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei106, Taiwan. LA - eng GR - NSTC 114-2622-8-002-016/National Science and Technology Council/ GR - NSTC 115-2112-M-002-007-/National Science and Technology Council/ GR - NSTC 115-2640-M-002-002-/National Science and Technology Council/ GR - NSTC 115-2740- M-002-007/National Science and Technology Council/ PT - Journal Article PL - United States TA - Nano Lett JT - Nano letters JID - 101088070 SB - IM OTO - NOTNLM OT - bismuth oxide (BiOx) OT - charge-transfer doping OT - contact engineering OT - field-effect transistors (FETs) OT - inverter OT - threshold-voltage engineering OT - transition-metal dichalcogenides (TMDs) OT - two-dimensional semiconductors EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 09:14 PHST- 2026/07/21 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:14 [entrez] AID - 5453865 [pii] AID - 10.1021/acs.nanolett.6c03585 [doi] PST - ppublish SO - Nano Lett. 2026 Oct 28;26(42):14675-14682. doi: 10.1021/acs.nanolett.6c03585. PMID- 42949397 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1530-6992 (Electronic) IS - 1530-6984 (Linking) VI - 26 IP - 42 DP - 2026 Oct 28 TI - Precursor-Free Photoelectrochemical Fabrication of Spiral Lead Oxide Nanostructures under Circularly Polarized Light. PG - 14717-14724 LID - 10.1021/acs.nanolett.6c03830 [doi] AB - Chiral nanostructures have attracted considerable attention because of their strong interactions with circularly polarized light (CPL) and their various applications. Although various fabrication methods have been reported, photoelectrochemical approaches using CPL as the chiral source are attractive because of their simplicity, cost-effectiveness, and scalability. However, previous CPL-based methods have required two or more steps, including the preparation of anisotropic precursors. Here, we demonstrate a single-step method for the selective growth of chiral PbO2 nanostructures with handedness dictated by CPL at a chiral yield of 87%, by simply irradiating a transparent electrode with CPL, without using precursor nanoparticles. The obtained structures exhibit well-defined spiral morphologies and high chiroptical activity with maximum dissymmetry factors (g-factors) ranging from 0.006 to 0.010. This conceptual framework, involving symmetry breaking through random nucleation and Mie-resonance-mediated spiral growth under CPL, may be applicable to the template-free fabrication of various chiral nanomaterials. CI - (c) 2026 American Chemical Society. FAU - Hayakawa, Ami AU - Hayakawa A AD - Graduate School of Science and Engineering, University of Toyama, 3190 Gofuku, Toyama930-8555, Japan. FAU - Tatsuma, Tetsu AU - Tatsuma T AUID- ORCID: 0000-0001-8738-9837 AD - Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo153-8505, Japan. FAU - Nishi, Hiroyasu AU - Nishi H AUID- ORCID: 0009-0004-7257-5601 AD - Graduate School of Science and Engineering, University of Toyama, 3190 Gofuku, Toyama930-8555, Japan. LA - eng GR - JP23H01932/Japan Society for the Promotion of Science/ GR - JP26K21860/Japan Society for the Promotion of Science/ PT - Journal Article PL - United States TA - Nano Lett JT - Nano letters JID - 101088070 SB - IM OTO - NOTNLM OT - Chiral nanostructures OT - Circularly polarized light OT - Lead oxide OT - Mie resonance OT - Photoelectrochemical nanofabrication EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 09:14 PHST- 2026/08/04 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:14 [entrez] AID - 5453818 [pii] AID - 10.1021/acs.nanolett.6c03830 [doi] PST - ppublish SO - Nano Lett. 2026 Oct 28;26(42):14717-14724. doi: 10.1021/acs.nanolett.6c03830. PMID- 42949380 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1530-6992 (Electronic) IS - 1530-6984 (Linking) VI - 26 IP - 42 DP - 2026 Oct 28 TI - Buoyancy-Mediated in Situ Phase-Transition Microbubbles Enable Efficient Aqueous Humor Drug Delivery for Accelerated Corneal Endothelial Regeneration. PG - 14578-14589 LID - 10.1021/acs.nanolett.6c03143 [doi] AB - The corneal endothelium plays a pivotal role in maintaining corneal clarity, and its damage leads to a hydration dysfunction and secondary glaucoma. We previously identified XMU-MP-1, a Hippo pathway inhibitor, as a potent stimulator of corneal endothelial cell proliferation in nonhuman primate models. However, its therapeutic potential is limited by poor intraocular permeability and low bioavailability. Herein, we report an engineered perfluoropentane (PFP)-cored lipid microbubble (MB) platform for the buoyancy-mediated targeted delivery of XMU-MP-1. Post intracameral injection, drug-loaded microbubbles (D@MB) undergo spontaneous in situ liquid-to-gas phase transition at physiological temperature, which grants them buoyancy-driven upward movement in aqueous humor to maintain continuous direct contact with the endothelial layer, achieving an extended retention of over 72 h in the anterior chamber. Finally, the D@MB drug delivery platform accelerates regenerative processes and restores both the corneal ultrastructure and physiological function. This study highlights a minimally invasive approach that bypasses traditional surgical interventions to stimulate the repair of the damaged corneal endothelium. CI - (c) 2026 American Chemical Society. FAU - Cai, Shundong AU - Cai S AD - Xiamen University Affiliated Xiamen Eye Center, Eye Institute of Xiamen University, School of Medicine, Xiamen University, Xiamen361102, China. AD - Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Fujian Engineering and Research Center of Eye Regenerative Medicine, Xiamen361102, China. FAU - Li, Mengdie AU - Li M AD - Xiamen University Affiliated Xiamen Eye Center, Eye Institute of Xiamen University, School of Medicine, Xiamen University, Xiamen361102, China. AD - Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Fujian Engineering and Research Center of Eye Regenerative Medicine, Xiamen361102, China. FAU - Zhuang, Jingbin AU - Zhuang J AD - Xiamen University Affiliated Xiamen Eye Center, Eye Institute of Xiamen University, School of Medicine, Xiamen University, Xiamen361102, China. AD - Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Fujian Engineering and Research Center of Eye Regenerative Medicine, Xiamen361102, China. FAU - Zhong, Meiqin AU - Zhong M AD - Xiamen University Affiliated Xiamen Eye Center, Eye Institute of Xiamen University, School of Medicine, Xiamen University, Xiamen361102, China. AD - Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Fujian Engineering and Research Center of Eye Regenerative Medicine, Xiamen361102, China. FAU - Zhang, Mingyou AU - Zhang M AD - Xiamen University Affiliated Xiamen Eye Center, Eye Institute of Xiamen University, School of Medicine, Xiamen University, Xiamen361102, China. AD - Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Fujian Engineering and Research Center of Eye Regenerative Medicine, Xiamen361102, China. FAU - Hu, Xu AU - Hu X AD - Xiamen University Affiliated Xiamen Eye Center, Eye Institute of Xiamen University, School of Medicine, Xiamen University, Xiamen361102, China. AD - Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Fujian Engineering and Research Center of Eye Regenerative Medicine, Xiamen361102, China. FAU - Ye, Jinfa AU - Ye J AD - School of Pharmacy, Xiamen Medical College, Xiamen361023, China. AD - State Key Laboratory of Physical Chemistry of Solid Surfaces & The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen361002, China. FAU - Han, Yun AU - Han Y AD - Xiamen University Affiliated Xiamen Eye Center, Eye Institute of Xiamen University, School of Medicine, Xiamen University, Xiamen361102, China. AD - Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Fujian Engineering and Research Center of Eye Regenerative Medicine, Xiamen361102, China. FAU - Su, Min AU - Su M AD - School of Pharmacy, Xiamen Medical College, Xiamen361023, China. FAU - Li, Wei AU - Li W AD - Xiamen University Affiliated Xiamen Eye Center, Eye Institute of Xiamen University, School of Medicine, Xiamen University, Xiamen361102, China. AD - Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Fujian Engineering and Research Center of Eye Regenerative Medicine, Xiamen361102, China. FAU - Liu, Gang AU - Liu G AUID- ORCID: 0000-0003-2613-7286 AD - State Key Laboratory of Physical Chemistry of Solid Surfaces & The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen361002, China. AD - State Key Laboratory of Cellular Stress Biology, School of Life Science, Xiamen University, Xiamen361102, China. FAU - Chu, Chengchao AU - Chu C AUID- ORCID: 0000-0002-1694-9363 AD - Xiamen University Affiliated Xiamen Eye Center, Eye Institute of Xiamen University, School of Medicine, Xiamen University, Xiamen361102, China. AD - Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Fujian Engineering and Research Center of Eye Regenerative Medicine, Xiamen361102, China. AD - State Key Laboratory of Physical Chemistry of Solid Surfaces & The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen361002, China. AD - State Key Laboratory of Cellular Stress Biology, School of Life Science, Xiamen University, Xiamen361102, China. LA - eng GR - 32271447/National Natural Science Foundation of China/ GR - 32401176/National Natural Science Foundation of China/ GR - 824B2054/National Natural Science Foundation of China/ GR - 2022J01021/Natural Science Foundation of Fujian Province/ GR - 2024J08337/Natural Science Foundation of Fujian Province/ GR - 2024J09005/Natural Science Foundation of Fujian Province/ GR - 2023YFB3810000/Major State Basic Research Development Program of China/ GR - 2023YFB3810002/Major State Basic Research Development Program of China/ GR - 2023YFB3810004/Major State Basic Research Development Program of China/ GR - 2024Y9727/Fujian Province Joint Funding Project for Scientific and Technological Innovation/ GR - K2023-17/Xiamen Medical College Research Fund/ PT - Journal Article PL - United States TA - Nano Lett JT - Nano letters JID - 101088070 RN - 0 (Fluorocarbons) RN - 678-26-2 (perfluoropentane) RN - 0 (Pentanes) SB - IM MH - Animals MH - Fluorocarbons/chemistry MH - *Endothelium, Corneal/drug effects/physiology MH - *Microbubbles MH - *Drug Delivery Systems MH - *Aqueous Humor/chemistry/metabolism MH - *Regeneration/drug effects MH - Phase Transition MH - Rabbits MH - Humans MH - Cell Proliferation/drug effects MH - Pentanes MH - Intracameral Injection OTO - NOTNLM OT - Corneal endothelial regeneration OT - Hippo pathway inhibitor OT - Microbubble OT - Perfluoropentane OT - Phase-transition OT - Tissue repair EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 09:14 PHST- 2026/06/26 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:14 [entrez] AID - 5451003 [pii] AID - 10.1021/acs.nanolett.6c03143 [doi] PST - ppublish SO - Nano Lett. 2026 Oct 28;26(42):14578-14589. doi: 10.1021/acs.nanolett.6c03143. PMID- 42949375 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1530-6992 (Electronic) IS - 1530-6984 (Linking) VI - 26 IP - 42 DP - 2026 Oct 28 TI - All-Optical Ultrafast Pure Spin Current in the Altermagnet Cr2SO. PG - 14535-14540 LID - 10.1021/acs.nanolett.6c02424 [doi] AB - All-optical generation of pure spin current horizontal line the flow of spin in the absence of a corresponding charge flow horizontal line relies on symmetry based compensation between the two spin channels. 2D d-wave altermagnets, which in many respects are ideal spintronic materials due to their very low spin-orbit coupling, possess a magnetic point group and highly anisotropic valley manifolds that would appear to preclude such current compensation, excluding them as materials for the ultrafast generation of pure spin current. Here we show that infrared valley excitation combined with a THz pulse envelope allows the generation of large and nearly 100% pure spin currents in the 2D altermagnet Cr2SO. Our approach is based on a valley selection rule coupling linearly polarized light to opposite spin valleys, along with the intrinsic momentum shift that a co-occurring THz pulse imbues a valley spin excitation with. These results provide a practical and all-optical route to the generation of pure spin current in 2D d-wave altermagnets, opening a path to lightwave control of spin in an environment with very low intrinsic spin-orbit coupling. CI - (c) 2026 American Chemical Society. FAU - Gill, Deepika AU - Gill D AUID- ORCID: 0000-0003-2299-1063 AD - Max-Born-Institute for Non-Linear Optics, Max-Born Strasse 2A, 12489Berlin, Germany. FAU - Wu, Ruikai AU - Wu R AD - Max-Born-Institute for Non-Linear Optics, Max-Born Strasse 2A, 12489Berlin, Germany. FAU - Chen, Wenhan AU - Chen W AD - Max-Planck-Institut fur Mikrostrukturphysik, Weinberg 2, D-06120Halle, Germany. FAU - Elliott, Peter AU - Elliott P AD - Scientific Computing Department, Science and Technology Facilities Council UK Research and Innovation (STFC-UKRI), Rutherford Appleton Laboratory, Harwell Campus, DidcotOX11 0QX, United Kingdom. FAU - Sharma, Sangeeta AU - Sharma S AD - Max-Born-Institute for Non-Linear Optics and Short Pulse Spectroscopy, Max-Born Strasse 2A, 12489Berlin, Germany. AD - Institute for Theoretical Solid-State Physics, Freie Universitat Berlin, Arnimallee 14, 14195Berlin, Germany. AD - Halle-Berlin-Regensburg Cluster of Excellence CCE, https://www.chiralelectronics.de/. FAU - Shallcross, Sam AU - Shallcross S AUID- ORCID: 0000-0003-4666-1501 AD - Max-Born-Institute for Non-Linear Optics, Max-Born Strasse 2A, 12489Berlin, Germany. LA - eng GR - 328545488 TRR227 - A04/Deutsche Forschungsgemeinschaft/ GR - SH-498-9/1/Deutsche Forschungsgemeinschaft/ GR - SPP2558 - 498/11-1/Deutsche Forschungsgemeinschaft/ GR - K612/2024/Leibniz-Gemeinschaft/ GR - P118/2021/Leibniz-Gemeinschaft/ PT - Journal Article PL - United States TA - Nano Lett JT - Nano letters JID - 101088070 SB - IM OTO - NOTNLM OT - altermagnets OT - lightwave spin control OT - pure spin current OT - terahertz pulses OT - valley selection rules EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 09:14 PHST- 2026/05/19 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:14 [entrez] AID - 5450569 [pii] AID - 10.1021/acs.nanolett.6c02424 [doi] PST - ppublish SO - Nano Lett. 2026 Oct 28;26(42):14535-14540. doi: 10.1021/acs.nanolett.6c02424. PMID- 42949372 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1530-6992 (Electronic) IS - 1530-6984 (Linking) VI - 26 IP - 42 DP - 2026 Oct 28 TI - Photobattery Architectures to Suppress Charge Recombination. PG - 14520-14528 LID - 10.1021/acs.nanolett.6c02373 [doi] AB - Integrating solar energy conversion and storage into single-unit photobatteries offers compact energy supplies. However, achieving sufficient photovoltage to charge batteries without external bias remains a critical bottleneck for these devices. Here, we demonstrate that the photovoltage in Zn-ion photobatteries can be modulated and enhanced by selecting and structuring the photoactive materials strategically. Randomly dispersed electrode configurations are easy to manufacture but they can suffer from charge recombination. In our experiments, these yielded a negligible photovoltage of approximately 0.01 V. In comparison, our proposed layer-by-layer design, integrating a solid-state dye-sensitized solar cell (DSSC) with an optimized spinel Zn-Mn oxide cathode, increased the photovoltage output to approximately 1 V under 1-sun illumination. This dramatic performance enhancement is achieved by better control over interfaces and charge transport and establishes a strategy for improving the architecture of future photobatteries. CI - (c) 2026 The Authors. Published by American Chemical Society. FAU - Shimokawa, Kohei AU - Shimokawa K AUID- ORCID: 0000-0003-3261-9679 AD - Institute for Manufacturing, Department of Engineering, University of Cambridge, CambridgeCB3 0FE, U.K. AD - Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Sendai980-8578, Japan. AD - Institute for Materials Research, Tohoku University, Sendai980-8577, Japan. FAU - Kim, Byung-Man AU - Kim BM AUID- ORCID: 0000-0002-4764-153X AD - Institute for Manufacturing, Department of Engineering, University of Cambridge, CambridgeCB3 0FE, U.K. FAU - Pujari, Arvind AU - Pujari A AUID- ORCID: 0000-0002-5415-3411 AD - Institute for Manufacturing, Department of Engineering, University of Cambridge, CambridgeCB3 0FE, U.K. AD - Cavendish Laboratory, Department of Physics, University of Cambridge, CambridgeCB3 0HE, U.K. FAU - Abbasi, Hooman AU - Abbasi H AD - Institute for Manufacturing, Department of Engineering, University of Cambridge, CambridgeCB3 0FE, U.K. FAU - Ichitsubo, Tetsu AU - Ichitsubo T AUID- ORCID: 0000-0002-1127-3034 AD - Institute for Materials Research, Tohoku University, Sendai980-8577, Japan. FAU - De Volder, Michael AU - De Volder M AD - Institute for Manufacturing, Department of Engineering, University of Cambridge, CambridgeCB3 0FE, U.K. LA - eng GR - 24K17516/Japan Society for the Promotion of Science/ GR - 26K01236/Japan Society for the Promotion of Science/ GR - 866005/Horizon 2020 European Commission (EC)/ PT - Journal Article PL - United States TA - Nano Lett JT - Nano letters JID - 101088070 SB - IM OTO - NOTNLM OT - Cathode materials OT - Energy harvesting and storage OT - Photobatteries OT - Photoelectrochemistry EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 09:13 PHST- 2026/05/17 00:00 [received] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/09/20 00:00 [revised] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:13 [entrez] AID - 5449469 [pii] AID - 10.1021/acs.nanolett.6c02373 [doi] PST - ppublish SO - Nano Lett. 2026 Oct 28;26(42):14520-14528. doi: 10.1021/acs.nanolett.6c02373. PMID- 42949371 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1530-6992 (Electronic) IS - 1530-6984 (Linking) VI - 26 IP - 42 DP - 2026 Oct 28 TI - Real-Time Single Molecule Imaging Reveals Pathway-Selective Action of Aducanumab on Amyloid-beta Aggregation. PG - 14560-14568 LID - 10.1021/acs.nanolett.6c02842 [doi] AB - Aducanumab is an anti-amyloid-beta (Abeta) antibody that binds aggregated Abeta species and has been reported to inhibit secondary nucleation on fibril surfaces. However, how antibody binding influences aggregate dynamics at the molecular level remains incompletely understood. Here, we used high-speed atomic force microscopy (HS-AFM) to directly visualize the interactions between aducanumab and Abeta42 aggregates in real time at single-molecule resolution. Aducanumab extensively decorated oligomers, protofibrils, and fibrils, with fibril binding depending on local surface structure. Consistent with its reported inhibition of secondary nucleation, bulk thioflavin T assays showed slowed aggregation. Nevertheless, in the continued presence of the antibody, protofibril formation proceeded horizontal line a pathway distinct from secondary nucleation. These observations reveal a pathway-selective mode of action: extensive surface binding and the suppression of secondary nucleation do not extend to protofibril formation, which proceeds in the continued presence of the antibody. CI - (c) 2026 American Chemical Society. FAU - Watanabe-Nakayama, Takahiro AU - Watanabe-Nakayama T AUID- ORCID: 0000-0001-9758-3975 AD - WPI Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa920-1192, Japan. FAU - Noguchi-Shinohara, Moeko AU - Noguchi-Shinohara M AD - Department of Neurology, Graduate School of Medical Sciences, Kanazawa University, 13-1, Takara-machi, Kanazawa920-8640, Japan. FAU - Umeda, Kenichi AU - Umeda K AUID- ORCID: 0000-0002-3650-3828 AD - WPI Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa920-1192, Japan. FAU - Muramatsu, Daiki AU - Muramatsu D AD - Department of Neurology, Graduate School of Medical Sciences, Kanazawa University, 13-1, Takara-machi, Kanazawa920-8640, Japan. FAU - Konno, Hiroki AU - Konno H AUID- ORCID: 0000-0002-1712-171X AD - WPI Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa920-1192, Japan. FAU - Kodera, Noriyuki AU - Kodera N AUID- ORCID: 0000-0003-4880-8423 AD - WPI Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa920-1192, Japan. FAU - Ando, Toshio AU - Ando T AUID- ORCID: 0000-0001-8819-154X AD - WPI Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa920-1192, Japan. FAU - Ono, Kenjiro AU - Ono K AUID- ORCID: 0000-0001-8454-6155 AD - Department of Neurology, Graduate School of Medical Sciences, Kanazawa University, 13-1, Takara-machi, Kanazawa920-8640, Japan. LA - eng GR - NA/Kanazawa University/ GR - JP19K07965/Japan Society for the Promotion of Science/ GR - JP22K07514/Japan Society for the Promotion of Science/ GR - NA/Ministry of Education, Culture, Sports, Science and Technology/ PT - Journal Article PL - United States TA - Nano Lett JT - Nano letters JID - 101088070 RN - 0 (Amyloid beta-Peptides) RN - 0 (Antibodies, Monoclonal, Humanized) RN - 105J35OE21 (aducanumab) RN - 0 (Protein Aggregates) RN - 0 (Peptide Fragments) RN - 0 (amyloid beta-protein (1-42)) SB - IM MH - *Amyloid beta-Peptides/chemistry/metabolism MH - *Antibodies, Monoclonal, Humanized/pharmacology/chemistry MH - Microscopy, Atomic Force/methods MH - *Single Molecule Imaging/methods MH - Humans MH - Protein Aggregates/drug effects MH - *Peptide Fragments/chemistry MH - *Alzheimer Disease/drug therapy MH - Protein Binding OTO - NOTNLM OT - Aducanumab OT - Alzheimer's disease OT - amyloid OT - high-speed atomic force microscopy OT - humanized monoclonal antibodies OT - single molecule imaging EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 09:13 PHST- 2026/06/10 00:00 [received] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/09/24 00:00 [revised] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:13 [entrez] AID - 5444291 [pii] AID - 10.1021/acs.nanolett.6c02842 [doi] PST - ppublish SO - Nano Lett. 2026 Oct 28;26(42):14560-14568. doi: 10.1021/acs.nanolett.6c02842. PMID- 42949366 OWN - NLM STAT- Publisher LR - 20261028 IS - 1875-9270 (Electronic) IS - 1051-9815 (Linking) DP - 2026 Oct 28 TI - Postural ergonomic risks during clinical dental training: A five-dimension observational analysis. PG - 10519815261492236 LID - 10.1177/10519815261492236 [doi] AB - BackgroundErgonomic problems are common in the field of dentistry due to musculoskeletal and neurological conditions that occur in various parts of the body as a result of poor posture and repetitive movements.ObjectivesTo analyze ergonomic errors by clinical area among dental students at a private university in Cusco.MethodsA cross-sectional observational study using secondary data was conducted, involving a population of 182 students selected according to specific criteria. A sample of 150 students from different areas of the dental clinic at a private university in Cusco, corresponding to the seventh, eighth, and ninth semesters, was analyzed. The RULA and REBA ergonomic scales were applied as assessment tools.ResultsThe study revealed that poor postures predominated in the pediatric dentistry clinical area across the five dimensions analyzed, followed by endodontics, restorative dentistry, periodontics, and oral rehabilitation.ConclusionsThere is a high prevalence of poor posture among dental students, highlighting the need for ergonomic education and preventive measures to reduce musculoskeletal injuries and improve the well-being of future dentists, regardless of their specialty. FAU - Gutierrez-Gayoso, Giovanna AU - Gutierrez-Gayoso G AD - Escuela Profesional de Estomatologia, Segunda Especialidad, Universidad Privada San Juan Bautista, Lima, Peru. RINGGOLD: 33222 FAU - Mamani Concha, Chandy AU - Mamani Concha C AUID- ORCID: 0009-0008-2380-776X AD - Escuela de Estomatologia, Universidad Andina del Cusco, Cusco, Peru. RINGGOLD: 187045 FAU - Aguilar Sumire, Vianeth G AU - Aguilar Sumire VG AUID- ORCID: 0009-0005-5617-5576 AD - Escuela Profesional de Estomatologia, Segunda Especialidad, Universidad Privada San Juan Bautista, Lima, Peru. RINGGOLD: 33222 FAU - Nieto Huaman, Cindy A AU - Nieto Huaman CA AUID- ORCID: 0009-0005-0245-9469 AD - Escuela de Estomatologia, Universidad Andina del Cusco, Cusco, Peru. RINGGOLD: 187045 FAU - Escobar Zabala, Oscar D AU - Escobar Zabala OD AUID- ORCID: 0000-0002-0875-7548 AD - Departamento de Estomatologia, Universidad Nacional de Chimborazo, Riobamba, Ecuador. RINGGOLD: 27899 LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Work JT - Work (Reading, Mass.) JID - 9204382 SB - IM OTO - NOTNLM OT - dentistry OT - ergonomics OT - musculoskeletal diseases OT - occupational health OT - posture OT - students dental EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 09:13 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:13 [entrez] AID - 10.1177/10519815261492236 [doi] PST - aheadofprint SO - Work. 2026 Oct 28:10519815261492236. doi: 10.1177/10519815261492236. PMID- 42949353 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1520-5118 (Electronic) IS - 0021-8561 (Linking) VI - 74 IP - 42 DP - 2026 Oct 28 TI - Decoupled Adult and Larval Host Preferences in Tephritid Fruit Flies with a Conserved Oviposition Attractant. PG - 32847-32857 LID - 10.1021/acs.jafc.6c09170 [doi] AB - Tephritid fruit flies exhibit considerable variability in host range, yet the chemosensory mechanisms underlying adult host selection and larval performance across species with contrasting dietary breadths remain unclear. Here, we investigated behavioral responses of adults and larvae of four species horizontal line Bactrocera dorsalis, B. correcta, Zeugodacus tau, and Z. cucurbitae horizontal line to seven fruit and vegetable cues. Adult preferences varied, with guava eliciting the strongest attraction in all species. Larval preferences were weaker and decoupled from adult choices. Comparative transcriptomics of guava-exposed antennae identified OBP19d as the most highly upregulated odorant-binding protein across species. RNAi knockdown of BdOBP19d in B. dorsalis reduced electrophysiological responses to guava volatiles and specifically abolished oviposition preference for 4-Ethylacetophenone. Molecular docking, electrophysiology, and behavioral assays collectively validated this interaction. These results reveal a conserved olfactory module for shared host attraction and identify 4-Ethylacetophenone as a candidate attractant for B. dorsalis females, with potential implications for pest management. CI - (c) 2026 American Chemical Society. FAU - Xu, Xiaomiao AU - Xu X AD - Key Laboratory of Agricultural Product Quality and Biosafety of the Ministry of Education, College of Plant Protection, Anhui Agricultural University, Hefei100081, China. FAU - Ma, Tonghui AU - Ma T AD - Key Laboratory of Agricultural Product Quality and Biosafety of the Ministry of Education, College of Plant Protection, Anhui Agricultural University, Hefei100081, China. FAU - Zhang, Lingwei AU - Zhang L AD - Key Laboratory of Agricultural Product Quality and Biosafety of the Ministry of Education, College of Plant Protection, Anhui Agricultural University, Hefei100081, China. FAU - Zhang, Yue AU - Zhang Y AD - Key Laboratory of Agricultural Product Quality and Biosafety of the Ministry of Education, College of Plant Protection, Anhui Agricultural University, Hefei100081, China. FAU - Yao, Jun AU - Yao J AD - Key Laboratory of Agricultural Product Quality and Biosafety of the Ministry of Education, College of Plant Protection, Anhui Agricultural University, Hefei100081, China. FAU - Wang, Jinsheng AU - Wang J AD - Key Laboratory of Agricultural Product Quality and Biosafety of the Ministry of Education, College of Plant Protection, Anhui Agricultural University, Hefei100081, China. FAU - Xu, Li AU - Xu L AD - Key Laboratory of Agricultural Product Quality and Biosafety of the Ministry of Education, College of Plant Protection, Anhui Agricultural University, Hefei100081, China. FAU - Zhan, Shuai AU - Zhan S AD - State Key Laboratory of Plant Trait Design, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai200032, China. FAU - Wang, Yaohui AU - Wang Y AUID- ORCID: 0000-0003-1903-1088 AD - Key Laboratory of Agricultural Product Quality and Biosafety of the Ministry of Education, College of Plant Protection, Anhui Agricultural University, Hefei100081, China. LA - eng GR - 2508085Y017/Natural Science Foundation of Anhui Province/ GR - 2023AH030045/Excellent Young Talents Fund Program of Higher Education Institutions of Anhui Province/ GR - 32202283/National Natural Science Foundation of China (NSFC)/ GR - 32225008/National Natural Science Foundation of China (NSFC)/ GR - 32530015/National Natural Science Foundation of China (NSFC)/ PT - Journal Article PL - United States TA - J Agric Food Chem JT - Journal of agricultural and food chemistry JID - 0374755 RN - 0 (Insect Proteins) RN - 0 (Receptors, Odorant) RN - 0 (Pheromones) RN - 0 (Acetophenones) RN - 0 (odorant-binding protein) SB - IM MH - Animals MH - *Tephritidae/physiology/genetics/growth & development/chemistry/metabolism MH - Female MH - Oviposition MH - Insect Proteins/genetics/metabolism/chemistry MH - Larva/physiology/genetics/growth & development MH - Receptors, Odorant/genetics/metabolism/chemistry MH - Fruit/parasitology/chemistry MH - *Pheromones/metabolism/chemistry MH - Psidium/chemistry/parasitology MH - Acetophenones/metabolism MH - Host Specificity OTO - NOTNLM OT - 4-Ethylacetophenone OT - OBP19d OT - Tephritidae fruit flies OT - host selection OT - oviposition behavior EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 09:13 PHST- 2026/06/21 00:00 [received] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:13 [entrez] AID - 5453813 [pii] AID - 10.1021/acs.jafc.6c09170 [doi] PST - ppublish SO - J Agric Food Chem. 2026 Oct 28;74(42):32847-32857. doi: 10.1021/acs.jafc.6c09170. PMID- 42949317 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1520-5118 (Electronic) IS - 0021-8561 (Linking) VI - 74 IP - 42 DP - 2026 Oct 28 TI - Clostridium butyricum Alleviates Meat Quality Deterioration in Eimeria-Infected Lambs Alongside Recovery of Thiamine Diphosphate-Dependent Glucose Metabolism. PG - 33405-33420 LID - 10.1021/acs.jafc.6c09444 [doi] AB - Eimeria spp. infection impairs lamb performance and meat quality, but the associated muscle metabolic basis and potential nutritional mitigation remains unclear. Twenty-four lambs were assigned to control (CON), Eimeria-infected (IF), and Eimeria-infected supplemented with Clostridium butyricum (CB; CB-IF) groups. Infection increased drip loss, and reduced cooking yield, whereas CB supplementation improved these traits. Metabolomics analysis revealed reduce thiamine diphosphate (TDP) and 4-methyl-5-hydroxyethylthiazole phosphate (HET-P) after infection, with partial recovery following CB supplementation. Integrated transcriptomic and WGCNA analysis identified coordinated associations among thiamine-related metabolites, glucose- and mitochondrial-metabolism-related gene signatures, and meat-quality traits. RT-qPCR supported the expression patterns of selected transcriptomic candidates. Collectively, CB supplementation alleviated selected meat-quality alterations and was associated with coordinated recovery of thiamine-related and glucose/mitochondrial metabolic signatures during Eimeria spp. infection. CI - (c) 2026 American Chemical Society. FAU - Liu, Manyu AU - Liu M AD - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - Key Laboratory of Quality and Safety Control of Poultry Products, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou450046, China. FAU - Guo, Lizhen AU - Guo L AD - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - Key Laboratory of Quality and Safety Control of Poultry Products, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou450046, China. FAU - Lin, Meiqi AU - Lin M AD - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - Key Laboratory of Quality and Safety Control of Poultry Products, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou450046, China. FAU - Li, Juanfeng AU - Li J AD - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - Key Laboratory of Quality and Safety Control of Poultry Products, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou450046, China. FAU - Zhen, Aoning AU - Zhen A AD - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - Key Laboratory of Quality and Safety Control of Poultry Products, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou450046, China. FAU - Li, Junqiang AU - Li J AD - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - Key Laboratory of Quality and Safety Control of Poultry Products, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou450046, China. FAU - Zhang, Longxian AU - Zhang L AUID- ORCID: 0000-0002-5706-131X AD - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - Key Laboratory of Quality and Safety Control of Poultry Products, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou450046, China. FAU - Li, Senyang AU - Li S AD - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - Key Laboratory of Quality and Safety Control of Poultry Products, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou450046, China. FAU - Huang, Shucheng AU - Huang S AUID- ORCID: 0000-0003-3163-8616 AD - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - Key Laboratory of Quality and Safety Control of Poultry Products, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. FAU - Jian, Fuchun AU - Jian F AUID- ORCID: 0000-0001-9481-2277 AD - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - Key Laboratory of Quality and Safety Control of Poultry Products, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou450046, China. AD - International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou450046, China. LA - eng GR - Z20251831016/Central Funds Guiding Local Science and Technology Development Special Project/ PT - Journal Article PL - United States TA - J Agric Food Chem JT - Journal of agricultural and food chemistry JID - 0374755 RN - IY9XDZ35W2 (Glucose) RN - Q57971654Y (Thiamine Pyrophosphate) SB - IM MH - Animals MH - *Glucose/metabolism MH - Sheep/metabolism MH - *Meat/analysis MH - *Clostridium butyricum/physiology MH - *Eimeria/physiology MH - *Thiamine Pyrophosphate/metabolism MH - *Sheep Diseases/parasitology/metabolism/microbiology MH - *Coccidiosis/veterinary/metabolism/parasitology MH - Animal Feed/analysis OTO - NOTNLM OT - Clostridium butyricum OT - Eimeria spp. OT - glucose metabolism OT - meat quality OT - multiomics OT - thiamine metabolism EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 09:13 PHST- 2026/06/25 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/09/25 00:00 [revised] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:13 [entrez] AID - 5450608 [pii] AID - 10.1021/acs.jafc.6c09444 [doi] PST - ppublish SO - J Agric Food Chem. 2026 Oct 28;74(42):33405-33420. doi: 10.1021/acs.jafc.6c09444. PMID- 42949299 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-3479 (Electronic) IS - 0020-7292 (Linking) DP - 2026 Oct 28 TI - Integrating large language models and expert knowledge to identify risk factors and predict mode of delivery with machine learning. LID - 10.1002/ijgo.71376 [doi] AB - BACKGROUND: Accurately predicting the mode of delivery is a significant challenge in obstetrics, with implications for maternal healthcare planning, resource allocation, and personalized patient counseling. While machine learning offers promising predictive capabilities, the selection of relevant clinical and socioeconomic risk factors from vast possibilities remains a critical and nontrivial step that directly affects model performance and clinical utility. OBJECTIVES: This study aimed to (1) predict the mode of delivery (vaginal vs. cesarean) using machine learning algorithms and (2) identify the key risk factors influencing delivery mode prediction. METHODS: A retrospective study was conducted on eligible pregnant women who delivered at Bent-Al-Huda Hospital in Bojnourd, Iran. A hybrid feature identification approach was employed: large language models (LLMs) initially proposed 63 potential risk factors. Through iterative refinement involving domain experts and assessment of data availability and frequency, this list was consolidated to 35 final variables for modeling. Machine learning algorithms, including multilayer perceptron (MLP), decision tree, naive Bayes, and support vector machine (SVM), were applied to build predictive models. RESULTS: Among all models, SVM showed the best performance using all features, achieving an accuracy of 94.8% and an area under the curve (AUC) of 0.94. Decision tree analysis identified previous cesarean section history, insurance type, and fetal presentation as the three most important predictive factors. After excluding 40 women with prior cesarean delivery, SVM maintained robust performance with accuracy of 94.3% and an AUC of 0.92. Using antenatal features only, MLP achieved the highest accuracy before and after excluding women with previous cesarean section (92.9% and 92.2%), while SVM also showed the highest discriminative ability (accuracy 92.4%, AUC 0.90 and accuracy 91.3%, AUC 0.87, respectively). CONCLUSION: This study demonstrates that combining expert knowledge with LLMs enables effective risk factor identification and that careful feature selection allows the proposed model to predict delivery type with high accuracy. CI - (c) 2026 International Federation of Gynecology and Obstetrics. FAU - Rashidi, Farzaneh AU - Rashidi F AUID- ORCID: 0000-0001-7497-4180 AD - Clinical Research Development Unit, Bentolhoda Hospital, North Khorasan University of Medical Sciences, Bojnurd, Iran. FAU - Qanbarzadeh, Saba AU - Qanbarzadeh S AD - Clinical Research Development Unit, Bentolhoda Hospital, North Khorasan University of Medical Sciences, Bojnurd, Iran. FAU - Orooji, Azam AU - Orooji A AUID- ORCID: 0000-0002-2773-6179 AD - Department of Medical Biotechnology, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Int J Gynaecol Obstet JT - International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics JID - 0210174 SB - IM OTO - NOTNLM OT - birth OT - classification OT - delivery OT - large language models OT - prediction EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 09:04 PHST- 2026/08/12 00:00 [revised] PHST- 2026/04/22 00:00 [received] PHST- 2026/09/01 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:04 [entrez] AID - 10.1002/ijgo.71376 [doi] PST - aheadofprint SO - Int J Gynaecol Obstet. 2026 Oct 28. doi: 10.1002/ijgo.71376. PMID- 42949293 OWN - NLM STAT- Publisher LR - 20261028 IS - 1098-5514 (Electronic) IS - 0022-538X (Linking) DP - 2026 Oct 28 TI - Functional analysis of non-muscle myosin heavy chain IIA (amino acids 1831-1882) in facilitating pseudorabies virus entry via interaction with glycoproteins gH and gL. PG - e0143226 LID - 10.1128/jvi.01432-26 [doi] AB - Pseudorabies virus (PRV) is the etiological agent responsible for porcine pseudorabies, causing substantial financial losses in the global swine industry. Non-muscle myosin heavy chain IIA (NMHC IIA), as an actin-binding motor protein, has been recognized as a functional factor in herpesvirus entry. However, its precise mechanism in PRV invasion remains poorly defined. Our investigation demonstrated that targeted inhibition of NMHC IIA, using a specific chemical inhibitor, siRNA-mediated knockdown, C-terminus of NMHC IIA (sPRA, amino acids [aa] 1672-1981), or rabbit anti-sPRA serum, significantly decreases PRV infection in PK-15 cells in a dose-dependent manner. During the early stage of PRV infection, NMHC IIA underwent rapid and pronounced translocation from the cytoplasm to the cell surface, which is beneficial to the virus entry. The study identified that sPRA mediates PRV internalization by binding to viral glycoproteins gH and gL. Furthermore, we pinpointed the subdomain spanning aa 1831-1882 within sPRA as the critical domain for both mediating the interaction with gH/gL and facilitating viral internalization. These results advance our comprehension of the molecular pathways through which NMHC IIA supports PRV infection and highlight its potential as a therapeutic target for PRV intervention. IMPORTANCE: Pseudorabies virus (PRV), a member of the Alphaherpesvirinae subfamily, not only poses an ongoing threat to the global swine industry but also presents a potential zoonotic risk. Although the host protein NMHC IIA has been identified as a critical factor mediating virus entry, its precise molecular mechanism during PRV infection is still unclear. Herein, we demonstrated that NMHC IIA participates in PRV internalization through binding to viral glycoproteins gH and gL. Moreover, we precisely mapped the critical functional region, spanning amino acids 1831-1882 in the C-terminus of NMHC IIA, responsible for both the viral entry process and the interaction with viral proteins. These findings could deepen the understanding of the interaction mechanism between PRV and the host, and provide a promising broad-spectrum antiviral target. FAU - Wang, Lizhen AU - Wang L AUID- ORCID: 0009-0001-8434-1421 AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China. RINGGOLD: 12469. ROR: https://ror.org/0051rme32 AD - Shaanxi Scientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture and Rural Affairs, Yangling, Shaanxi, China. ROR: https://ror.org/05ckt8b96 FAU - Wang, Lei AU - Wang L AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China. RINGGOLD: 12469. ROR: https://ror.org/0051rme32 AD - Shaanxi Scientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture and Rural Affairs, Yangling, Shaanxi, China. ROR: https://ror.org/05ckt8b96 FAU - Ren, Binbin AU - Ren B AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China. RINGGOLD: 12469. ROR: https://ror.org/0051rme32 AD - Shaanxi Scientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture and Rural Affairs, Yangling, Shaanxi, China. ROR: https://ror.org/05ckt8b96 FAU - Zhao, Jiakai AU - Zhao J AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China. RINGGOLD: 12469. ROR: https://ror.org/0051rme32 AD - Shaanxi Scientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture and Rural Affairs, Yangling, Shaanxi, China. ROR: https://ror.org/05ckt8b96 FAU - Zhang, Lu AU - Zhang L AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China. RINGGOLD: 12469. ROR: https://ror.org/0051rme32 AD - Shaanxi Scientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture and Rural Affairs, Yangling, Shaanxi, China. ROR: https://ror.org/05ckt8b96 FAU - Chen, Xu AU - Chen X AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China. RINGGOLD: 12469. ROR: https://ror.org/0051rme32 AD - Shaanxi Scientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture and Rural Affairs, Yangling, Shaanxi, China. ROR: https://ror.org/05ckt8b96 FAU - Tang, Ting AU - Tang T AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China. RINGGOLD: 12469. ROR: https://ror.org/0051rme32 AD - Shaanxi Scientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture and Rural Affairs, Yangling, Shaanxi, China. ROR: https://ror.org/05ckt8b96 FAU - Zhao, Qin AU - Zhao Q AUID- ORCID: 0000-0002-8993-7494 AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China. RINGGOLD: 12469. ROR: https://ror.org/0051rme32 AD - Shaanxi Scientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture and Rural Affairs, Yangling, Shaanxi, China. ROR: https://ror.org/05ckt8b96 FAU - Sun, Yani AU - Sun Y AUID- ORCID: 0000-0002-2943-9151 AD - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China. RINGGOLD: 12469. ROR: https://ror.org/0051rme32 AD - Shaanxi Scientific Observing and Experimental Station of Veterinary Pharmacology and Diagnostic Technology, Ministry of Agriculture and Rural Affairs, Yangling, Shaanxi, China. ROR: https://ror.org/05ckt8b96 LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Virol JT - Journal of virology JID - 0113724 SB - IM OTO - NOTNLM OT - gH/gL OT - non-muscle myosin heavy chain IIA OT - pseudorabies virus OT - virus internalization EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 09:04 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:04 [entrez] AID - 10.1128/jvi.01432-26 [doi] PST - aheadofprint SO - J Virol. 2026 Oct 28:e0143226. doi: 10.1128/jvi.01432-26. PMID- 42949287 OWN - NLM STAT- Publisher LR - 20261028 IS - 2332-4260 (Electronic) IS - 2332-4252 (Linking) DP - 2026 Oct 28 TI - Standardized Occipital Artery-Posterior Cerebral Artery Bypass With Encephalo-Duro-Pericranial Synangiosis Using a Cortical P4 Recipient for Posterior Circulation-Involved Moyamoya Disease. LID - 10.1227/ons.0000000000002227 [doi] AB - BACKGROUND AND OBJECTIVES: Posterior cerebral artery involvement in moyamoya disease (MMD) is associated with occipital hypoperfusion, visual disturbance, recurrent ischemic events, and worse neurological status. In selected patients, posterior circulation-targeted revascularization may represent an important component of surgical management. We evaluated outcomes after a standardized occipital artery-posterior cerebral artery (OA-PCA) bypass strategy using a cortical P4 recipient combined with encephalo-duro-pericranial synangiosis (EDPS) in patients with posterior circulation-involved MMD. METHODS: We retrospectively reviewed consecutive posterior circulation revascularization procedures performed from August 2012 through December 2024. All procedures used a standardized OA-PCA bypass to a cortical P4 recipient combined with EDPS. The primary outcome was bypass patency on follow-up imaging. Secondary outcomes included collateral formation, perfusion improvement, early visual improvement, modified Rankin Scale improvement, and procedure-related perioperative adverse events. RESULTS: A total of 113 procedures in 109 patients were included. Early postoperative computed tomography angiography confirmed bypass patency in all evaluable procedures (112/112, 100%). At 12 months or later, bypass patency was observed in 59 of 102 procedures (57.8%), collateral formation in 63 of 102 procedures (61.8%, grades A-B), and perfusion improvement in 63 of 102 procedures (61.8%). Among 72 procedures with documented preoperative visual disturbance, early visual improvement occurred in 31 (43.1%). Modified Rankin Scale improvement of at least 1 point at last follow-up occurred in 69 of 113 procedures (61.1%). Procedure-related perioperative adverse events were infrequent, and no bypass-related reoperation occurred within 30 days. CONCLUSION: In this single-center retrospective series, standardized OA-PCA bypass using a cortical P4 recipient combined with EDPS was associated with reliable early graft patency and favorable angiographic, perfusion, and clinical outcomes in selected patients with posterior circulation-involved MMD. Late graft status should be interpreted together with collateral development, perfusion findings, and clinical course rather than in isolation. CI - Copyright (c) 2026 The Author(s). Published by Wolters Kluwer Health, LLC on behalf of Congress of Neurological Surgeons. FAU - Huang, Ying AU - Huang Y AUID- ORCID: 0000-0001-6606-7935 AD - Department of Neurosurgery, Aviation General Hospital, Beijing, China. FAU - Chang, Jia-Yi AU - Chang JY AD - Department of Neurosurgery, Aviation General Hospital, Beijing, China. FAU - Feng, Zeng-Wei AU - Feng ZW AD - Department of Neurosurgery, Aviation General Hospital, Beijing, China. FAU - Zuo, Yun-Long AU - Zuo YL AD - Department of Neurosurgery, Aviation General Hospital, Beijing, China. FAU - Zuo, Fu-Xing AU - Zuo FX AD - Department of Neurosurgery, Aviation General Hospital, Beijing, China. FAU - Yu, Li-Ping AU - Yu LP AD - Department of Neurosurgery, Aviation General Hospital, Beijing, China. FAU - Xue, Ling AU - Xue L AD - Department of Neurosurgery, Aviation General Hospital, Beijing, China. FAU - Chen, Guo-Qiang AU - Chen GQ AD - Department of Neurosurgery, Aviation General Hospital, Beijing, China. FAU - Han, Hong-Yan AU - Han HY AD - Department of Neurosurgery, Aviation General Hospital, Beijing, China. FAU - Kuroda, Satoshi AU - Kuroda S AD - Department of Neurosurgery, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama, Toyama, Japan. LA - eng GR - No. JB2019-02/Moyamoya Disease Research Laboratory Grant of Aviation General Hospital/ PT - Journal Article DEP - 20261028 PL - United States TA - Oper Neurosurg JT - Operative neurosurgery (Hagerstown, Md.) JID - 101635417 SB - IM OTO - NOTNLM OT - Encephalo-duro-pericranial synangiosis OT - Moyamoya disease OT - Occipital artery-posterior cerebral artery bypass OT - Posterior cerebral artery OT - Posterior circulation OT - Revascularization EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 09:04 PHST- 2026/06/18 00:00 [received] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:04 [entrez] AID - 01787389-990000000-02187 [pii] AID - 10.1227/ons.0000000000002227 [doi] PST - aheadofprint SO - Oper Neurosurg. 2026 Oct 28. doi: 10.1227/ons.0000000000002227. PMID- 42949284 OWN - NLM STAT- Publisher LR - 20261028 IS - 2165-0497 (Electronic) IS - 2165-0497 (Linking) DP - 2026 Oct 28 TI - Laboratory characteristics associated with microbial identification quality: a national multicenter study based on external quality assessment across China. PG - e0290026 LID - 10.1128/spectrum.02900-26 [doi] AB - Clinical microbiology laboratories are essential for infectious disease diagnosis and antimicrobial stewardship. However, data on laboratory capacity and quality gaps across tertiary hospitals in China remain limited. This multicenter cross-sectional study aimed to evaluate associations between laboratory characteristics and microbial identification performance, and to develop an exploratory prediction model for attainment of the program-defined mutual recognition threshold. Data were collected from 183 tertiary hospital microbiology laboratories across 31 provinces in China. Thirteen baseline laboratory characteristics, including institutional scale, staffing, equipment, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) availability, and ISO 15189 accreditation, were analyzed in relation to microbial identification performance assessed through a standardized national external quality assessment scheme. Correlation analysis, multivariable linear regression, K-means clustering, and logistic regression were used to evaluate associations, performance heterogeneity, and attainment of the program-defined mutual recognition threshold. MALDI-TOF MS availability showed the strongest and most consistent associations with identification performance and was positively associated in adjusted models with fastidious bacteria (beta = 9.84, 95% CI 3.65-16.02; P = 0.002), rare bacteria (beta = 7.23, 95% CI 4.03-10.43; P < 0.001), yeast (beta = 7.16, 95% CI 1.66-12.66; P = 0.011), and total identification scores (beta = 45.44, 95% CI 5.38-85.50; P = 0.026). Hospital bed capacity was associated only with yeast identification, while biosafety cabinet number was associated with Salmonella serotyping performance. Cluster analysis identified three performance tiers, supporting the presence of heterogeneity in microbial identification capability. The exploratory prediction model retained MALDI-TOF MS availability and conventional incubator number and showed moderate discrimination (AUC = 0.755), with a sensitivity of 92.9% and specificity of 50.0% at the optimal cutoff probability of 0.524. These findings indicate that technology availability and selected laboratory characteristics are associated with microbial identification performance and may help inform targeted laboratory quality improvement strategies. IMPORTANCE: Reliable microbial identification underpins infectious disease diagnosis, antimicrobial stewardship, and infection control. However, evidence directly linking routine laboratory resources to standardized identification performance remains limited. In this national multicenter study, standardized external quality assessment results were integrated with 13 baseline laboratory characteristics from 183 tertiary hospital microbiology laboratories across 31 provinces in China. matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) availability emerged as the laboratory characteristic most consistently associated with microbial identification performance, particularly for fastidious bacteria, rare bacteria, yeast, and overall identification scores. Exploratory clustering further demonstrated substantial heterogeneity in performance across laboratories, while a prediction model incorporating MALDI-TOF MS availability and conventional incubator number showed moderate discrimination for attainment of the mutual recognition threshold. These findings provide quantitative evidence that technology availability and broader laboratory capacity characteristics are associated with identification quality and may help guide targeted quality improvement and capacity-building efforts. FAU - Wang, Yaxuan AU - Wang Y AUID- ORCID: 0009-0008-3021-4129 AD - National Center for Clinical Laboratories, Beijing Hospital, National Center for Gerontology; National Clinical Research Center for Gerontology; The Key Laboratory of Geriatrics of NHC; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, People's Republic of China. RINGGOLD: 12501. ROR: https://ror.org/02drdmm93 FAU - Hu, Jihong AU - Hu J AD - National Center for Clinical Laboratories, Beijing Hospital, National Center for Gerontology; National Clinical Research Center for Gerontology; The Key Laboratory of Geriatrics of NHC; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, People's Republic of China. RINGGOLD: 12501. ROR: https://ror.org/02drdmm93 FAU - Pian, Yaya AU - Pian Y AD - National Center for Clinical Laboratories, Beijing Hospital, National Center for Gerontology; National Clinical Research Center for Gerontology; The Key Laboratory of Geriatrics of NHC; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, People's Republic of China. RINGGOLD: 12501. ROR: https://ror.org/02drdmm93 FAU - Zhang, Ran AU - Zhang R AD - National Center for Clinical Laboratories, Beijing Hospital, National Center for Gerontology; National Clinical Research Center for Gerontology; The Key Laboratory of Geriatrics of NHC; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, People's Republic of China. RINGGOLD: 12501. ROR: https://ror.org/02drdmm93 FAU - Gao, Zhenxiang AU - Gao Z AD - National Center for Clinical Laboratories, Beijing Hospital, National Center for Gerontology; National Clinical Research Center for Gerontology; The Key Laboratory of Geriatrics of NHC; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, People's Republic of China. RINGGOLD: 12501. ROR: https://ror.org/02drdmm93 FAU - Liu, Wei AU - Liu W AD - National Center for Clinical Laboratories, Beijing Hospital, National Center for Gerontology; National Clinical Research Center for Gerontology; The Key Laboratory of Geriatrics of NHC; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, People's Republic of China. RINGGOLD: 12501. ROR: https://ror.org/02drdmm93 FAU - Liu, Yudong AU - Liu Y AUID- ORCID: 0000-0002-5153-3400 AD - National Center for Clinical Laboratories, Beijing Hospital, National Center for Gerontology; National Clinical Research Center for Gerontology; The Key Laboratory of Geriatrics of NHC; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, People's Republic of China. RINGGOLD: 12501. ROR: https://ror.org/02drdmm93 FAU - Ma, Jie AU - Ma J AUID- ORCID: 0000-0002-2938-818X AD - National Center for Clinical Laboratories, Beijing Hospital, National Center for Gerontology; National Clinical Research Center for Gerontology; The Key Laboratory of Geriatrics of NHC; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, People's Republic of China. RINGGOLD: 12501. ROR: https://ror.org/02drdmm93 LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Microbiol Spectr JT - Microbiology spectrum JID - 101634614 SB - IM OTO - NOTNLM OT - MALDI-TOF MS OT - clinical microbiology OT - external quality assessment OT - laboratory characteristics OT - microbial identification OT - quality improvement EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 09:04 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:04 [entrez] AID - 10.1128/spectrum.02900-26 [doi] PST - aheadofprint SO - Microbiol Spectr. 2026 Oct 28:e0290026. doi: 10.1128/spectrum.02900-26. PMID- 42949278 OWN - NLM STAT- Publisher LR - 20261028 IS - 1744-8360 (Electronic) IS - 1473-7175 (Linking) DP - 2026 Oct 28 TI - Acute anxiety states in emergency neuropsychiatry: diagnostic boundaries, time-sensitive mechanisms, and rapid-acting therapeutic strategies. PG - 1-16 LID - 10.1080/14737175.2026.2751476 [doi] AB - INTRODUCTION: Acute anxiety states (AAS) impose a disproportionate burden on emergency neuropsychiatry. Nevertheless, they remain under-standardized in triage and rapid-phase management and are often misclassified among high-risk medical mimics. AREAS COVERED: This narrative review is based on literature retrieved from PubMed, Embase, ClinicalTrials.gov, and the Cochrane Library (January 2000-September 2026), and addresses diagnostic boundaries, acute versus chronic neurobiological mechanisms, current pharmacotherapy limitations, and emerging rapid-acting agents. This article reconceptualizes AAS as a transdiagnostic, time-sensitive phenotype rather than a discrete nosological entity. It critically appraises DSM-5-TR/ICD-11 diagnostic boundaries, GRACE-1-aligned triage for non-cardiac chest pain, and age-specific screening including 4AT and GAI-SF. The authors review the acute neurobiology distinguishing AAS from trait anxiety - transient glutamatergic-GABAergic remodeling, neurosteroid depletion, non-genomic HPA feedback, and amygdala-PFC-BNST circuit dysregulation - alongside rapid-acting candidates including MM-120 (now redesignated DT120), LYT-300, and subunit-selective GABAkines. EXPERT OPINION: Reflexively discharging AAS after cardiac rule-out perpetuates somatic hypervigilance and forfeits cardiovascular risk mitigation. However, extrapolating provocation-paradigm efficacy of psychoplastogens and pherines to chaotic ED cohorts is premature; regulatory, safety, equity, and false-positive concerns surrounding biomarker-driven closed-loop platforms remain unresolved. Over the next 5-10 years, genuine translational progress will be defined by mechanism-targeted anxiolytics validated in unselected acute-care populations, not by digital phenotyping alone. FAU - Wei, Yumei AU - Wei Y AD - Shanghai Key Laboratory of Psychotic Disorders, Brain Health Institute, National Center for Mental Disorder, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine and School of Psychology, Shanghai, China. FAU - Shi, Zhongyong AU - Shi Z AD - Department of Psychiatry, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China. FAU - Shen, Yuan AU - Shen Y AD - Shanghai Key Laboratory of Psychotic Disorders, Brain Health Institute, National Center for Mental Disorder, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine and School of Psychology, Shanghai, China. AD - Department of Psychiatry, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - Expert Rev Neurother JT - Expert review of neurotherapeutics JID - 101129944 SB - IM OTO - NOTNLM OT - Acute anxiety states OT - emergency psychiatry OT - neurosteroids OT - non-cardiac chest pain OT - psychoplastogens OT - rapid-acting anxiolytics EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 09:03 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:03 [entrez] AID - 10.1080/14737175.2026.2751476 [doi] PST - aheadofprint SO - Expert Rev Neurother. 2026 Oct 28:1-16. doi: 10.1080/14737175.2026.2751476. PMID- 42949275 OWN - NLM STAT- Publisher LR - 20261028 IS - 1098-6618 (Electronic) IS - 0893-8512 (Linking) DP - 2026 Oct 28 TI - The global landscape of Campylobacter: genomic epidemiology, AMR trends, and the path to vaccine development. PG - e0019126 LID - 10.1128/cmr.00191-26 [doi] AB - SUMMARYCampylobacter species, particularly Campylobacter jejuni and Campylobacter coli, are leading causes of bacterial gastroenteritis worldwide, responsible for an estimated 500 million cases of acute diarrheal illness annually. Despite their global prevalence and association with severe post-infectious sequelae such as Guillain-Barre syndrome, no licensed vaccine exists, and the growing threat of antimicrobial resistance further complicates clinical management. This review synthesizes current knowledge on the epidemiology, genomic diversity, antimicrobial resistance mechanisms, and vaccine development strategies for Campylobacter species, with emphasis on global trends and public health relevance in the United States. Through a One Health lens, we examine transmission across human, animal, and environmental reservoirs, and highlight the role of integrated genomic surveillance in tracking clonal expansion, horizontal gene transfer, and resistance dissemination. We also examine historical and current approaches to vaccine development, including whole-cell, subunit, glycoconjugate, and genomics-guided antigen discovery strategies. Challenges such as antigenic variability, phase variation, and inadequate experimental models have impeded progress, though recent genomics-informed interventions show promise in both poultry and human contexts. Continued investment in genomic surveillance, antimicrobial resistance, and rational antigen selection will be essential for developing effective vaccines and reducing the global burden of campylobacteriosis. FAU - Downham, Rachael L AU - Downham RL AUID- ORCID: 0009-0004-0207-9637 AD - Department of Cellular & Molecular Biology, University of Hawai;i John A. Burns School of Medicine, Honolulu, Hawai;i, USA. FAU - Dombach, Jamie L AU - Dombach JL AD - Department of Microbiology, Tripler Army Medical Center, Honolulu, Hawai;i, USA. RINGGOLD: 8015. ROR: https://ror.org/05p4q8207 FAU - MacArthur, John L AU - MacArthur JL AD - Department of Microbiology, Tripler Army Medical Center, Honolulu, Hawai;i, USA. RINGGOLD: 8015. ROR: https://ror.org/05p4q8207 FAU - Norris, Michael H AU - Norris MH AD - Pathogen Analysis and Translational Health Group, School of Life Sciences, University of Hawai;i at Manoa, Honolulu, Hawai;i, USA. RINGGOLD: 3949. ROR: https://ror.org/01wspgy28 FAU - Ewers, Evan C AU - Ewers EC AD - Department of Medicine, Tripler Army Medical Center, Honolulu, Hawai;i, USA. RINGGOLD: 8015. ROR: https://ror.org/05p4q8207 AD - Department of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA. RINGGOLD: 1685. ROR: https://ror.org/04r3kq386 FAU - Le Saux, Olivier L P AU - Le Saux OLP AD - Department of Cellular & Molecular Biology, University of Hawai;i John A. Burns School of Medicine, Honolulu, Hawai;i, USA. FAU - Tribble, David R AU - Tribble DR AD - Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA. RINGGOLD: 1685. ROR: https://ror.org/04r3kq386 FAU - Kamau, Edwin AU - Kamau E AUID- ORCID: 0000-0002-5761-7883 AD - Department of Microbiology, Tripler Army Medical Center, Honolulu, Hawai;i, USA. RINGGOLD: 8015. ROR: https://ror.org/05p4q8207 LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Clin Microbiol Rev JT - Clinical microbiology reviews JID - 8807282 SB - IM OTO - NOTNLM OT - Campylobacter OT - One Health OT - epidemiology OT - genomics OT - resistance OT - surveillance OT - vaccine targets OT - vaccines OT - virulence OT - zoonoses EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 09:03 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:03 [entrez] AID - 10.1128/cmr.00191-26 [doi] PST - aheadofprint SO - Clin Microbiol Rev. 2026 Oct 28:e0019126. doi: 10.1128/cmr.00191-26. PMID- 42949272 OWN - NLM STAT- Publisher LR - 20261028 IS - 1944-8252 (Electronic) IS - 1944-8244 (Linking) DP - 2026 Oct 28 TI - Silver-Cluster and Polyoxometalate-Incorporated Metal-Organic Frameworks for Electrochemical Nitrite Sensing and Capacitive Energy Storage. LID - 10.1021/acsami.6c18102 [doi] AB - In this work, four new polyoxometalate-based metal-organic frameworks (POMOFs), namely [Ag10HCl2(dtb)8(PMo12O40)3].2H2O (JOU-42-Mo), [Ag10HCl2(dtb)8(PW12O40)3].2H2O (JOU-42-W), [Ag4Cl(dtb)2PMo12O40].2H2O (JOU-43-Mo), and [Ag4(OH)(H2O)2(dtb)2PW12O40] (JOU-43-W) (dtb = 4,4'-(1,4-phenylene)bis(4H-1,2,4-triazole)), constructed from silver clusters and Keggin-type polyoxometalates (POMs), were synthesized via a solvothermal strategy. Single-crystal X-ray diffraction analyses reveal that these compounds consist of two structural families. The JOU-42 series features a three-dimensional (3D) fcu-type framework assembled from decanuclear Ag clusters, generating tetrahedral and octahedral cages for the confinement of POM guests, whereas the JOU-43 series adopts a 2D + 2D --> 3D interpenetrated architecture containing one-dimensional channels for POM encapsulation. The combination of redox-active POM clusters and accessible Ag sites endows these POMOFs with enhanced electrochemical sensing performance toward nitrite (NO2-). Among them, JOU-42-Mo exhibits the best NO2- sensing performance, achieving a wide linear detection range of 20-30,000 mumol L-1 and a low detection limit of 3.88 mumol L-1, together with excellent selectivity, reproducibility, and stability. Furthermore, the POMOF electrodes display promising capacitive energy storage characteristics, demonstrating the multifunctional potential of Ag-cluster-based POMOFs. CI - (c) 2026 American Chemical Society. FAU - Zhao, Li-Ting AU - Zhao LT AD - School of Environmental and Chemical Engineering, Jiangsu Ocean University, Lianyungang, Jiangsu222005, China. FAU - Zhang, Lin AU - Zhang L AD - School of Environmental and Chemical Engineering, Jiangsu Ocean University, Lianyungang, Jiangsu222005, China. FAU - Xiang, Ke-Jie AU - Xiang KJ AD - School of Environmental and Chemical Engineering, Jiangsu Ocean University, Lianyungang, Jiangsu222005, China. FAU - Luo, Yu-Hui AU - Luo YH AUID- ORCID: 0000-0002-2597-0017 AD - School of Environmental and Chemical Engineering, Jiangsu Ocean University, Lianyungang, Jiangsu222005, China. FAU - Zhang, Dong-En AU - Zhang DE AD - School of Environmental and Chemical Engineering, Jiangsu Ocean University, Lianyungang, Jiangsu222005, China. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - ACS Appl Mater Interfaces JT - ACS applied materials & interfaces JID - 101504991 SB - IM OTO - NOTNLM OT - electrochemical sensing OT - metal-organic frameworks OT - nitrite detection OT - polyoxometalate OT - silver cluster OT - supercapacitor EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 09:03 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:03 [entrez] AID - 5459830 [pii] AID - 10.1021/acsami.6c18102 [doi] PST - aheadofprint SO - ACS Appl Mater Interfaces. 2026 Oct 28. doi: 10.1021/acsami.6c18102. PMID- 42949271 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2473-4209 (Electronic) IS - 0094-2405 (Linking) VI - 53 IP - 11 DP - 2026 Nov TI - Can we assess brain perfusion on a regional basis from digital subtraction angiography images with motion artifacts? LID - e70728 [pii] LID - 10.1002/mp.70728 [doi] AB - BACKGROUND: A method called IPEN (for Intra-operative PErfusion assessment with No gantry rotation) has been developed that uses x-ray angiography images and assesses volumetric soft tissue perfusion on a regional basis. It has the potential to enable quantitative cross-sectional perfusion assessment before, during, and after interventional procedures, such that physicians can assess the risk and outcome of the procedure. However, the current IPEN will not function if there is no native (non-subtracted) x-ray angiography images available and digital subtraction angiography (DSA) images are corrupted by motion artifacts. PURPOSE: To demonstrate that region-based tissue enhancement can be extracted from DSA images even if they are contaminated by motion artifacts. METHODS: We developed a motion-compensating IPEN framework using DSA images (called MCI-D) and validated its core formulation. A key breakthrough enabling MCI-D was the finding that, even when DSA images contain motion artifacts, subtracting two DSA images can isolate the change in tissue enhancement between the images if motion is absent between the two times. RESULTS: A qualitative assessment using computer simulated XCAT perfusion phantom data validated the finding, and clinical data supported the feasibility. An extensive, systematic, and quantitative assessment showed that MCI-D was highly correlated to the ground truth (e.g., the correlation coefficients > 0.99 for both the time-enhancement curve and the blood flow perfusion index, maximum slope of the rising part of the time-enhancement curve). CONCLUSIONS: MCI-D enables accurate motion-compensated perfusion assessment from DSA images, even in the presence of severe motion artifacts, provided that the motion is not continuous and occurs within a short period of time. CI - (c) 2026 American Association of Physicists in Medicine. FAU - Taguchi, Katsuyuki AU - Taguchi K AD - The Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. FAU - Subramanian, Shalini AU - Subramanian S AD - Department of Biomedical Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. FAU - Segars, W Paul AU - Segars WP AD - Carl E. Ravin Advanced Imaging Laboratories, Department of Radiology, Duke University, Durham, North Carolina, USA. FAU - Faria, Andreia V AU - Faria AV AD - The Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. LA - eng GR - R21 EB029049/NH/NIH HHS/United States GR - R01 NS126256/NH/NIH HHS/United States GR - P41 EB031771/NH/NIH HHS/United States GR - R01 EB001838/NH/NIH HHS/United States GR - P41 EB028744/NH/NIH HHS/United States PT - Journal Article PL - United States TA - Med Phys JT - Medical physics JID - 0425746 SB - IM MH - *Angiography, Digital Subtraction MH - *Artifacts MH - Humans MH - Movement MH - *Brain/diagnostic imaging/blood supply MH - *Image Processing, Computer-Assisted/methods MH - Phantoms, Imaging MH - *Cerebrovascular Circulation MH - Motion OTO - NOTNLM OT - computed tomography OT - intervention OT - perfusion OT - x-ray system EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 09:03 PHST- 2026/09/22 00:00 [revised] PHST- 2026/04/21 00:00 [received] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:03 [entrez] AID - e70728 [pii] AID - 10.1002/mp.70728 [doi] PST - ppublish SO - Med Phys. 2026 Nov;53(11):e70728. doi: 10.1002/mp.70728. PMID- 42949267 OWN - NLM STAT- Publisher LR - 20261028 IS - 2165-0497 (Electronic) IS - 2165-0497 (Linking) DP - 2026 Oct 28 TI - Genomic determinants of fluoroquinolone resistance and delafloxacin activity in Staphylococcus epidermidis isolates causing implant-associated bone and joint infections. PG - e0081426 LID - 10.1128/spectrum.00814-26 [doi] AB - Implant-associated bone and joint infections (IA-BJIs) due to Staphylococcus epidermidis are increasingly problematic because of biofilm formation and rising fluoroquinolone resistance. Delafloxacin is a recently approved fluoroquinolone with promising activity against resistant staphylococci; however, genomic determinants of susceptibility in S. epidermidis from IA-BJI remain insufficiently characterized. The aim of this study was to characterize delafloxacin in vitro activity against S. epidermidis clinical isolates from IA-BJI and to define the genomic determinants underlying reduced susceptibility to delafloxacin. This retrospective multicenter study (2022-2026) including 47 S. epidermidis isolates recovered from IA-BJI. Antimicrobial susceptibility was determined by broth microdilution/MIC strips. Multilocus sequence typing, QRDR substitutions (gyrA, gyrB, parC, parE), efflux pump genes, and the presence of the biofilm-associated ica operon were characterized by whole-genome sequencing (MiSeq, Illumina). Thirty-five isolates were levofloxacin-resistant. Among these, delafloxacin MIC(50/90) were 0.38 and 1.5 mg/L; 49% (17/35) met the Staphylococcus aureus MIC NSB --> PHA --> AN. Theoretical calculations reveal that CoFe optimizes the electronic structure to modulate the surface coverage of active hydrogen (*H) from water dissociation, thereby promoting the hydrogenation reaction while efficiently suppressing the competing hydrogen evolution reaction. Coupling this cathode with 5-hydroxymethylfurfural (HMF) oxidation to produce 2,5-furandicarboxylic acid (FDCA) gives a higher energy utilization efficiency and economic returns than the conventional system that coupled with the oxygen evolution reaction. This work demonstrates a promising strategy for upgrading both nitroaromatic and biomass-derived substrates via green electrosynthesis, offering a viable route toward the energy-efficient and economically attractive co-production of fine chemicals. CI - (c) 2026 American Chemical Society. FAU - Tao, Jingzhe AU - Tao J AD - Department of Applied Chemistry, College of Chemical Engineering, State Key Laboratory of Advanced Separation Membrane Materials, Zhejiang University of Technology, Hangzhou310014, P. R. China. FAU - Wang, Weijian AU - Wang W AD - Department of Applied Chemistry, College of Chemical Engineering, State Key Laboratory of Advanced Separation Membrane Materials, Zhejiang University of Technology, Hangzhou310014, P. R. China. FAU - Diao, Zihao AU - Diao Z AD - Department of Applied Chemistry, College of Chemical Engineering, State Key Laboratory of Advanced Separation Membrane Materials, Zhejiang University of Technology, Hangzhou310014, P. R. China. FAU - Dou, Haoyun AU - Dou H AUID- ORCID: 0009-0003-5359-4316 AD - College of Physics and Electronics Information, Yunnan Key Laboratory of Optoelectronic Information Technology, Key Laboratory of Advanced Technique & Preparation for Renewable Energy Materials, Yunnan Normal University, Kunming650500, P. R. China. FAU - Wang, Zhengyu AU - Wang Z AD - Department of Applied Chemistry, College of Chemical Engineering, State Key Laboratory of Advanced Separation Membrane Materials, Zhejiang University of Technology, Hangzhou310014, P. R. China. FAU - Shi, Xiaowei AU - Shi X AUID- ORCID: 0000-0002-8948-072X AD - Department of Applied Chemistry, College of Chemical Engineering, State Key Laboratory of Advanced Separation Membrane Materials, Zhejiang University of Technology, Hangzhou310014, P. R. China. FAU - Wang, Hong-En AU - Wang HE AUID- ORCID: 0000-0002-6859-5683 AD - College of Physics and Electronics Information, Yunnan Key Laboratory of Optoelectronic Information Technology, Key Laboratory of Advanced Technique & Preparation for Renewable Energy Materials, Yunnan Normal University, Kunming650500, P. R. China. FAU - Zheng, Lingxia AU - Zheng L AUID- ORCID: 0000-0002-5358-9133 AD - Department of Applied Chemistry, College of Chemical Engineering, State Key Laboratory of Advanced Separation Membrane Materials, Zhejiang University of Technology, Hangzhou310014, P. R. China. LA - eng GR - 22575217/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261028 PL - United States TA - ACS Appl Mater Interfaces JT - ACS applied materials & interfaces JID - 101504991 SB - IM OTO - NOTNLM OT - active hydrogen species coverage modulation OT - co-electrolysis system OT - electrocatalytic hydrogenation OT - nitrobenzene electroreduction OT - non-precious catalysts EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 09:03 PHST- 2026/07/28 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 09:03 [entrez] AID - 5459877 [pii] AID - 10.1021/acsami.6c15490 [doi] PST - aheadofprint SO - ACS Appl Mater Interfaces. 2026 Oct 28. doi: 10.1021/acsami.6c15490. PMID- 42949228 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1940-087X (Electronic) IS - 1940-087X (Linking) IP - 236 DP - 2026 Oct 27 TI - The Diagnostic Value and Molecular Mechanism of miR-1271-5p Combined with Ultrasound in Endometriosis. LID - 10.3791/73384 [doi] AB - Endometriosis (EMs) is a systemic inflammatory disease characterized by the growth of endometrial tissue outside the uterus. Its underlying mechanisms remain unclear, contributing to limitations in diagnosis and treatment. This study evaluated the diagnostic value of serum miR-1271-5p combined with transvaginal ultrasound (TVUS) and examined the regulatory relationship between miR-1271-5p and insulin-like growth factor 1 receptor (IGF1R). The study included 115 patients with EMs and 90 healthy volunteers. MiR-1271-5p and IGF1R expression levels were measured using real-time quantitative polymerase chain reaction (RT-qPCR). Receiver operating characteristic (ROC) analysis was used to assess the diagnostic performance of miR-1271-5p and TVUS individually and in combination. A dual-luciferase reporter assay was used to verify the interaction between miR-1271-5p and IGF1R, and in vitro experiments were conducted using hEM15A and 12Z cells. The combined use of miR-1271-5p and TVUS demonstrated high diagnostic performance for EMs. MiR-1271-5p negatively regulated IGF1R, and inhibition of miR-1271-5p significantly reduced cell proliferation, migration, and invasion. Silencing IGF1R reversed these effects. Serum IGF1R mRNA expression was reduced in patients with EMs and was inversely correlated with miR-1271-5p expression. These findings support the diagnostic value of serum miR-1271-5p combined with TVUS and indicate that miR-1271-5p regulates EMs-associated cellular behavior through IGF1R. FAU - Ye, Liqin AU - Ye L AD - Department of Ultrasound Medicine, The First Hospital of Putian City. FAU - Zhang, Yuanyuan AU - Zhang Y AD - Outpatient Department, Shanghai Changzheng Hospital, The Second Affiliated Hospital of Naval Medical University. FAU - Zheng, Qiuhua AU - Zheng Q AD - Ultrasound Department, The Fourth Affiliated Hospital of Guangzhou Medical University. FAU - Hu, Ting AU - Hu T AD - Ultrasound Department, The Central Hospital of Shangrao. FAU - Xu, Linlin AU - Xu L AD - Department of Midwifery, Weifang Nursing Vocational College; Linlinxu_wf@163.com. LA - eng PT - Journal Article PT - Video-Audio Media DEP - 20261027 PL - United States TA - J Vis Exp JT - Journal of visualized experiments : JoVE JID - 101313252 RN - 0 (MicroRNAs) RN - 0 (MIRN1271 microRNA, human) RN - EC 2.7.10.1 (Receptor, IGF Type 1) RN - 0 (IGF1R protein, human) RN - 0 (Receptors, Somatomedin) SB - IM MH - Humans MH - Female MH - *MicroRNAs/blood/genetics MH - *Endometriosis/diagnostic imaging/blood/diagnosis/genetics MH - Ultrasonography/methods MH - Receptor, IGF Type 1/genetics/biosynthesis MH - Adult MH - Receptors, Somatomedin/genetics EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 08:43 PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:43 [entrez] AID - 10.3791/73384 [doi] PST - epublish SO - J Vis Exp. 2026 Oct 27;(236). doi: 10.3791/73384. PMID- 42949213 OWN - NLM STAT- Publisher LR - 20261028 IS - 2326-5205 (Electronic) IS - 2326-5191 (Linking) DP - 2026 Oct 28 TI - The development and validation of a new diagnostic sonographic enthesitis score for psoriatic arthritis: Diagnostic Ultrasound Enthesitis Tool (DUET). LID - 10.1002/art.70384 [doi] AB - OBJECTIVE: The Diagnostic Ultrasound Enthesitis Tool (DUET) study aimed to develop and validate a new sonographic scoring system for enthesitis to aid PsA diagnosis. METHODS: We prospectively enrolled patients with early PsA, psoriasis without arthritis (PsO), and non-inflammatory musculoskeletal symptoms as controls (CTL) from 17 centers. A standardized ultrasound assessment of 16 entheseal sites was performed and images were centrally scored by three blinded readers for inflammatory and structural entheseal lesions. Using a stepwise approach with logistic regression models stratified by age, we identified optimal lesion combinations, weightings, and entheseal sites for distinguishing PsA from CTL. Age-specific cut-off points were selected to maintain specificity above 70%. Performance was assessed in a validation cohort of PsA and CTL. RESULTS: We included 213 PsA, 100 PsO, and 106 CTL in the discovery cohort. The final DUET score combined weighted inflammatory (hypoechogenicity + thickening + Doppler grade x 2) and structural (enthesophyte grade + erosion x 3) scores at three entheseal sites: Achilles, proximal patellar tendon insertion, and triceps. Age stratification (55 years) improved discriminative performance. In the discovery cohort, DUET achieved an AUC of 0.65, with specificity of 73-74% and sensitivity of 47-49%. In an external validation cohort (101 PsA, 69 CTL), specificity improved to 74-100% with similar sensitivity (49-52%). Sensitivity increased up to 63% in patients with tender entheses. CONCLUSION: DUET is a novel, externally validated sonographic enthesitis score that could support the diagnostic assessment of patients with early PsA, particularly in individuals with tender entheses. CI - (c) 2026 American College of Rheumatology. FAU - Eder, Lihi AU - Eder L AUID- ORCID: 0000-0002-1473-1715 AD - Women's College Hospital, University of Toronto, Toronto, ON, Canada. FAU - Ribeiro, Andre L AU - Ribeiro AL AUID- ORCID: 0000-0002-6782-8924 AD - Hospital Moinhos de Vento, and Hospital de Clinicas de Porto Alegre, Porto Alegre, Brazil. FAU - de Toledo, Ricardo Acayaba AU - de Toledo RA AD - Centro Integrado de Pesquisas do Hospital de Base de Sao Jose do Rio Preto, Sao Jose do Rio Preto Medical School, Brazil. FAU - Bakewell, Catherine AU - Bakewell C AD - Intermountain Healthcare Medical Group, Salt Lake City, UT, USA. FAU - Carron, Philippe AU - Carron P AUID- ORCID: 0000-0001-9254-6171 AD - Department of Rheumatology, University Hospital Ghent, Gent, Belgium; Center for Inflammation Research, Molecular Immunology and Inflammation Unit, VIB-Ghent University, Zwijnaarde, Belgium. FAU - Elnady, Basant AU - Elnady B AUID- ORCID: 0000-0002-5908-0151 AD - Department of Rheumatology, Benha University, Benha, Egypt; Department of Rheumatology, Al Hada Armed Forces Hospital, Taif, Saudi Arabia. FAU - Haddad, Amir AU - Haddad A AD - Carmel Medical Center, Ruth and Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel. FAU - Kavanaugh, Arthur AU - Kavanaugh A AD - Division of rheumatology, autoimmunity, and immunology; the University of California, San Diego, San Diego, CA, USA. FAU - Katz, Arnon AU - Katz A AD - GRAPPA Patient Research Partner group, Haifa, Israel. FAU - Kohler, Minna J AU - Kohler MJ AD - Massachusetts General Hospital, Harvard Medical School, Boston, USA. FAU - Marin, Josefina AU - Marin J AD - Hospital Rey Juan Carlos, Madrid, Spain. FAU - Afgani, Fahmeen AU - Afgani F AD - Women's College Hospital, Toronto, ON, Canada. FAU - Malliotis, Nikolas AU - Malliotis N AD - Section of Rheumatology, Uppsala University Hospital, Uppsala, Sweden. FAU - Nguyen, Nam AU - Nguyen N AUID- ORCID: 0000-0002-7669-7081 AD - Department of Medicine, University of Toronto, Toronto, ON, Canada. FAU - Nzeusseu, Adrien AU - Nzeusseu A AD - Department of Rheumatology, Cliniques Universitaires Saint-Luc; Institut de Recherche Experimentale et Clinique (IREC), Universite catholique de Louvain, Brussels, Belgium. FAU - Polachek, Ari AU - Polachek A AUID- ORCID: 0000-0002-2411-8084 AD - Institute of Rheumatology, Rabin medical center - Beilinson hospital, and Department of Rheumatology, Sourasky Medical Center, Gray Faculty of Medical and Health Sciences, Tel-Aviv University, Tel-Aviv, Israel. FAU - Rodriguez, Ernesto AU - Rodriguez E AD - Florida Medical Clinic, Zephyrhills, FL, USA. FAU - Rosemffet, Marcos AU - Rosemffet M AD - Psychophysical Rehabilitation Institute, Buenos Aires, Argentina. FAU - Singh, Abha AU - Singh A AD - Division of Rheumatology, University of Arizona College of Medicine, Banner University Medical Center, Phoenix, AZ, USA. FAU - Stoenoiu, Maria S AU - Stoenoiu MS AD - Department of Rheumatology, Cliniques Universitaires Saint-Luc; Institut de Recherche Experimentale et Clinique (IREC), Universite catholique de Louvain, Brussels, Belgium. FAU - Szentpetery, Agnes AU - Szentpetery A AUID- ORCID: 0000-0002-2962-8964 AD - Rheumatology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden. FAU - Tinazzi, Ilaria AU - Tinazzi I AD - Unit of Rheumatology, Ospedale Sacro Cuore-Don Calabria, Verona, Italy. FAU - Yinh, Janeth AU - Yinh J AD - Massachusetts General Hospital, Harvard Medical School, USA. FAU - Yang, Mu AU - Yang M AD - University of Waterloo, Waterloo, ON, Canada. FAU - McGonagle, Dennis AU - McGonagle D AD - University of Leeds, Leeds, UK. FAU - Cook, Richard AU - Cook R AD - University of Waterloo, Waterloo, ON, Canada. FAU - Kaeley, Gurjit S AU - Kaeley GS AD - Division of Rheumatology, University of Florida, Jacksonville, FL, USA. FAU - Aydin, Sibel Z AU - Aydin SZ AD - University of Ottawa Faculty of Medicine, Ottawa Hospital Research Institute, Ottawa, ON, Canada. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Arthritis Rheumatol JT - Arthritis & rheumatology (Hoboken, N.J.) JID - 101623795 SB - IM EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 08:35 PHST- 2026/09/17 00:00 [revised] PHST- 2026/01/31 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:35 [entrez] AID - 10.1002/art.70384 [doi] PST - aheadofprint SO - Arthritis Rheumatol. 2026 Oct 28. doi: 10.1002/art.70384. PMID- 42949206 OWN - NLM STAT- Publisher LR - 20261029 IS - 1467-7652 (Electronic) IS - 1467-7644 (Print) IS - 1467-7644 (Linking) DP - 2026 Oct 28 TI - Natural Variation in CYCLIC NUCLEOTIDE-GATED ION CHANNEL 16 Controls Fruit Weight in Tomato. LID - 10.1111/pbi.70782 [doi] AB - Fruit weight is a crucial agronomic trait influencing yield and quality in tomato domestication. Although numerous quantitative trait loci (QTLs) controlling fruit weight have been reported, the underlying molecular mechanisms remain largely unresolved. Here, we identify natural variation in the promoter region of CYCLIC NUCLEOTIDE-GATED ION CHANNEL 16 (CNGC16) associated with both fruit weight and gene expression differences via genome-wide association study (GWAS) in tomato accessions. Geographical and subpopulation analyses reveal that the large-fruit CNGC16 haplotype was selectively favoured during domestication and may contribute to local environmental adaptation. Functional assays demonstrate that CNGC16 regulates fruit weight by promoting cell division and maintaining cytosolic Ca(2+) homeostasis in fruit cells. Mechanistically, CNGC16 physically and genetically interacts with calmodulin 6 (CaM6) to mediate calcium signalling that controls fruit size. These findings uncover a novel calcium-dependent pathway regulating tomato fruit weight and propose CNGC16 and CaM6 as promising molecular targets for crop improvement. CI - (c) 2026 The Author(s). Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. FAU - Wang, Xiang AU - Wang X AUID- ORCID: 0009-0006-2727-0862 AD - College of Horticultural Science, Zhejiang A&F University, Zhejiang, China. FAU - Huang, Yixuan AU - Huang Y AD - College of Horticultural Science, Zhejiang A&F University, Zhejiang, China. FAU - Miao, Huaiqi AU - Miao H AD - College of Horticultural Science, Zhejiang A&F University, Zhejiang, China. FAU - Dai, Ying AU - Dai Y AD - College of Horticultural Science, Zhejiang A&F University, Zhejiang, China. FAU - Wang, Youwei AU - Wang Y AD - College of Horticultural Science, Zhejiang A&F University, Zhejiang, China. FAU - Ding, Ziyuan AU - Ding Z AD - College of Horticultural Science, Zhejiang A&F University, Zhejiang, China. FAU - Li, Nanyi AU - Li N AD - College of Horticultural Science, Zhejiang A&F University, Zhejiang, China. FAU - Wu, Gang AU - Wu G AUID- ORCID: 0000-0002-6876-3886 AD - College of Horticultural Science, Zhejiang A&F University, Zhejiang, China. AD - College of Life Sciences, Zhejiang Normal University, Zhejiang, China. LA - eng GR - 32502685/National Natural Science Foundation of China/ GR - LQ24C150001/Zhejiang Provincial Natural Science Foundation of China/ GR - Zhejiang Normal University/ PT - Journal Article DEP - 20261028 PL - England TA - Plant Biotechnol J JT - Plant biotechnology journal JID - 101201889 SB - IM PMC - PMC13715367 OTO - NOTNLM OT - CNGC16 OT - CaM6 OT - GWAS OT - calcium OT - domestication OT - fruit weight OT - natural variation OT - tomato COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/28 CRDT- 2026/10/28 08:35 PHST- 2026/10/12 00:00 [revised] PHST- 2025/11/04 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:35 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - PBI70782 [pii] AID - 10.1111/pbi.70782 [doi] PST - aheadofprint SO - Plant Biotechnol J. 2026 Oct 28:10.1111/pbi.70782. doi: 10.1111/pbi.70782. PMID- 42949205 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1940-087X (Electronic) IS - 1940-087X (Linking) IP - 236 DP - 2026 Oct 27 TI - Immune-Related Biomarkers and an Exploratory Prognostic Signature in Biliary Tract and Gallbladder Cancer. LID - 10.3791/72007 [doi] AB - Gallbladder cancer is an aggressive biliary tract malignancy associated with poor prognosis and limited molecularly guided treatment options. Chronic inflammation and immune dysregulation play central roles in gallbladder carcinogenesis; however, reproducible computational workflows for identifying immune-related biomarkers remain limited. This study describes an integrated bioinformatic workflow for identifying immune-related differentially expressed genes (IRDEGs) and developing an exploratory prognostic gene signature. Differential expression analysis was performed using dataset-specific R-based workflows, and differentially expressed genes were intersected with immune-related gene sets obtained from ImmPort, InnateDB, and the Molecular Signatures Database (MSigDB). Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and gene set enrichment analyses were conducted to characterize biological functions and signaling pathways. Protein-protein interaction networks were constructed using STRING and Cytoscape; immune-cell infiltration was estimated using CIBERSORT; and prognostic genes were identified through sequential univariate, least absolute shrinkage and selection operator (LASSO) Cox, and multivariable Cox regression analyses. The analysis identified 824 consensus differentially expressed genes, including 312 IRDEGs. These genes were significantly enriched in inflammatory and immune-regulatory pathways, including IL6/JAK/STAT3, nuclear factor of kappa light chain enhancer (NF-kappaB), Toll-like receptor, and chemokine signaling pathways. CXCL8, IL1B, MMP9, TLR2, S100A8, and S100A9 were identified as key hub genes. Immune-cell infiltration analysis revealed a markedly altered tumor immune microenvironment. An exploratory five-gene prognostic signature comprising CXCL8, S100A9, IL16, MMP9, and CD36 successfully stratified patients according to overall survival risk. This integrated workflow provides a reproducible framework for identifying immune-related biomarkers and developing exploratory prognostic signatures in gallbladder cancer. However, independent clinical cohorts and experimental validation are required before these findings can be translated into clinical applications. FAU - Zhou, Zhe AU - Zhou Z AD - Department of General Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine. AD - Shanghai Key Laboratory of Biliary Tract Disease Research. AD - Research Institute of Biliary Tract Disease, Shanghai Jiao Tong University School of Medicine. FAU - Yang, Yue AU - Yang Y AD - Department of General Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine. AD - Shanghai Key Laboratory of Biliary Tract Disease Research. AD - Research Institute of Biliary Tract Disease, Shanghai Jiao Tong University School of Medicine. FAU - Yang, Ziyi AU - Yang Z AD - Department of General Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine. AD - Shanghai Key Laboratory of Biliary Tract Disease Research. AD - Research Institute of Biliary Tract Disease, Shanghai Jiao Tong University School of Medicine. FAU - Liu, Shilei AU - Liu S AD - Department of General Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine. AD - Shanghai Key Laboratory of Biliary Tract Disease Research. AD - Research Institute of Biliary Tract Disease, Shanghai Jiao Tong University School of Medicine. FAU - Gong, Wei AU - Gong W AD - Department of General Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine. AD - Shanghai Key Laboratory of Biliary Tract Disease Research. AD - Research Institute of Biliary Tract Disease, Shanghai Jiao Tong University School of Medicine; gong19702026@hotmail.com. LA - eng PT - Journal Article PT - Video-Audio Media DEP - 20261027 PL - United States TA - J Vis Exp JT - Journal of visualized experiments : JoVE JID - 101313252 RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - *Gallbladder Neoplasms/immunology/genetics MH - *Biomarkers, Tumor/genetics/immunology MH - Prognosis MH - *Biliary Tract Neoplasms/immunology/genetics MH - Gene Expression Profiling/methods EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 08:35 PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:35 [entrez] AID - 10.3791/72007 [doi] PST - epublish SO - J Vis Exp. 2026 Oct 27;(236). doi: 10.3791/72007. PMID- 42949204 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1940-087X (Electronic) IS - 1940-087X (Linking) IP - 236 DP - 2026 Oct 27 TI - Oncogenic Role of PLOD3 in Melanoma Progression via Wnt/beta-catenin Signaling and Tumor Microenvironment Reprogramming. LID - 10.3791/73234 [doi] AB - Melanoma is an aggressive malignancy, and treatment strategies remain limited for patients with advanced disease, highlighting the need for a better understanding of the driving mechanisms. This study aimed to identify genes associated with melanoma tumorigenesis, characterize their potential mechanisms, and explore the therapeutic relevance and druggability of selected candidates through experimental and computational approaches. Melanoma-related hub genes were screened by integrating weighted gene co-expression network analysis (WGCNA) with least absolute shrinkage and selection operator (LASSO) regression analysis. The biological functions of the candidate gene were validated using proliferation and migration assays in melanoma cells. RNA sequencing was applied to investigate the underlying molecular mechanism, whereas CIBERSORT and xCell analyses were used to investigate the relationship between PLOD3 and immune infiltration. Structure-based virtual screening was performed to explore candidate PLOD3-binding compounds. PLOD3, C4orf48, BUB1, and CD300LF were identified as a melanoma-related gene signature, among which PLOD3 exhibited the strongest association with melanoma progression and metastasis. PLOD3 was significantly upregulated in melanoma and positively correlated with epithelial-mesenchymal transition (EMT) signatures across multiple independent cohorts. Loss-of-function experiments revealed reduced proliferation, migration, and invasion of melanoma cells following PLOD3 depletion. PLOD3 expression was also associated with Wnt/beta-catenin pathway activity, and treatment with a pharmacological Wnt/beta-catenin activator partially restored the cellular phenotypes suppressed by PLOD3 silencing. Furthermore, elevated PLOD3 expression was associated with reduced CD8(+) T-cell infiltration and an immunosuppressive tumor microenvironment. Structure-based virtual screening identified four candidate compounds-Rosamultin, Actein, Temsirolimus, and Punicalagin-with strong predicted binding affinity to the catalytic glycosyltransferase (GT) domain of PLOD3, supporting its target potential. PLOD3 functions as a critical driver of melanoma progression by promoting Wnt/beta-catenin signaling and shaping an immunosuppressive tumor microenvironment. These findings identify PLOD3 as a promising therapeutic target and provide candidate compounds for further investigation in melanoma-targeted therapy. FAU - Xu, Haifeng AU - Xu H AD - Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology; tanakh1989@163.com. FAU - Zhang, Yu AU - Zhang Y AD - Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology. LA - eng PT - Journal Article PT - Video-Audio Media DEP - 20261027 PL - United States TA - J Vis Exp JT - Journal of visualized experiments : JoVE JID - 101313252 SB - IM MH - Humans MH - *Melanoma/pathology/genetics/metabolism MH - *Tumor Microenvironment/physiology MH - *Wnt Signaling Pathway/physiology MH - Cell Line, Tumor MH - Disease Progression EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 08:34 PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:34 [entrez] AID - 10.3791/73234 [doi] PST - epublish SO - J Vis Exp. 2026 Oct 27;(236). doi: 10.3791/73234. PMID- 42949202 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1651-2081 (Electronic) IS - 1650-1977 (Linking) VI - 58 DP - 2026 Oct 28 TI - Patient-reported outcome measures for depressive symptoms in adults with spinal cord injury: a COSMIN systematic review. PG - jrm46626 LID - 10.2340/jrm.v58.46626 [doi] AB - OBJECTIVE: To evaluate the measurement properties of patient-reported measures of depressive symptoms in adults with spinal cord injury and inform their purpose-specific selection and interpretation in clinical and research settings. DESIGN: Systematic review. SUBJECTS/PATIENTS: Adults with traumatic or non-traumatic spinal cord injury. METHODS: 10 databases were searched from inception to 25 June 2026, with reference checking, citation tracking, instrument websites, and targeted searches. Two reviewers independently selected reports, extracted data, assessed risk of bias, rated measurement properties, and graded certainty using current international guidance for outcome measurement instruments. RESULTS: 28 reports evaluated 27 separately scored versions, forms, or depression subscales from 14 instrument families. The Spinal Cord Injury-Quality of Life Depression Item Bank had the strongest condition-specific development and internal-structure evidence, but evidence gaps and insufficient measurement invariance prevented recommendation. The English Patient Health Questionnaire-9 had sufficient case-finding evidence but insufficient structural validity for interpreting its conventional total score as a clearly unidimensional severity measure. The English depression subscale of the Hospital Anxiety and Depression Scale showed sufficient structural validity, internal consistency, and construct validity. Evidence for measurement error and responsiveness was scarce. CONCLUSION: No measure had sufficiently complete evidence for routine use across screening, severity assessment, and change measurement. FAU - Lu, Runlin AU - Lu R AUID- ORCID: 0009-0007-6479-9981 AD - Department of Spine and Orthopedics, Tianyou Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, Hubei, China. FAU - Dun, Yixin AU - Dun Y AUID- ORCID: 0009-0009-2504-6447 AD - Department of Spine and Orthopedics, Tianyou Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, Hubei, China. FAU - Li, Lingzhi AU - Li L AUID- ORCID: 0009-0009-5070-6320 AD - 1Department of Spine and Orthopedics, Tianyou Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, Hubei, China. FAU - Hu, Bing AU - Hu B AUID- ORCID: 0009-0003-5090-1229 AD - Department of Spine and Orthopedics, Tianyou Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, Hubei, China. 15549484980@163.com. FAU - Zhang, Caihong AU - Zhang C AUID- ORCID: 0000-0002-7260-1636 AD - Liyuan Hospital of Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei, China. LA - eng PT - Journal Article PT - Systematic Review DEP - 20261028 PL - Sweden TA - J Rehabil Med JT - Journal of rehabilitation medicine JID - 101088169 SB - IM MH - Humans MH - *Spinal Cord Injuries/psychology/complications MH - *Patient Reported Outcome Measures MH - *Depression/diagnosis/etiology MH - Quality of Life MH - Adult EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 08:34 PHST- 2026/08/01 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:34 [entrez] AID - 10.2340/jrm.v58.46626 [doi] PST - epublish SO - J Rehabil Med. 2026 Oct 28;58:jrm46626. doi: 10.2340/jrm.v58.46626. PMID- 42949200 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1768-322X (Electronic) IS - 0248-4900 (Linking) VI - 118 IP - 11 DP - 2026 Nov TI - FAM60A Regulates SIN3A-HDAC Complex Activity to Induce G1/S Phase Arrest and Promote Differentiation of Low-Grade Glioma. LID - e70088 [pii] LID - 10.1111/boc.70088 [doi] AB - BACKGROUND: Low-grade gliomas (LGGs) exhibit dysregulated cell-cycle progression, contributing to tumor growth. Family with sequence similarity 60 member A (FAM60A), a component of the SIN3A-HDAC epigenetic complex, is implicated in cell proliferation and stemness, but its role in glioma remains unclear. METHODS: Human glioblastoma U87 MG cells and SW1088 (grade III astrocytoma) were used to investigate the function of FAM60A. Serum starvation was applied to synchronize cells in the G1 phase. Cellular proliferation, migration, and invasion capacities were examined using the CCK-8 and Transwell assays. The distribution of cells within various phases of the cell cycle was assessed through flow cytometry. HDAC activity, histone acetylation, and protein interactions were examined by fluorometric assay, Western blotting, and co-immunoprecipitation. Rescue experiments with the HDAC inhibitor were used to evaluate functional interplay among FAM60A, SIN3A, and HDAC. RESULTS: FAM60A depletion inhibited proliferation, induced G1/S arrest, downregulated Cyclin D1/CDK4/6, and upregulated p21/p27, while reducing migration and stemness. Overexpression had opposite effects. Mechanistically, FAM60A promoted the expression and activity of the SIN3A-HDAC complex, thereby reducing histone acetylation and facilitating the transcriptional repression of cell-cycle inhibitors. SIN3A knockdown or HDAC inhibition recapitulated FAM60A loss, and their combination further enhanced G1 arrest, cell-cycle inhibitor upregulation, histone acetylation, and differentiation. CONCLUSION: FAM60A promotes LGG cell proliferation by facilitating SIN3A-HDAC-mediated transcriptional repression of cell-cycle inhibitors. Targeting FAM60A or the SIN3A-HDAC complex may represent a potential therapeutic strategy for controlling LGG growth. CI - (c) 2026 Societe Francaise des Microscopies and Societe Biologie Cellulaire de France. FAU - Feng, Yucheng AU - Feng Y AD - Department of Neurosurgery, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China. FAU - Yan, Zeying AU - Yan Z AD - Department of Neurosurgery, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China. FAU - Chen, Hongwu AU - Chen H AD - Department of Neurosurgery, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China. FAU - Huang, Jiehao AU - Huang J AD - Department of Critical Care Medicine, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China. FAU - Xu, Yimin AU - Xu Y AD - Department of Neurosurgery, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China. FAU - Lin, Rui AU - Lin R AUID- ORCID: 0009-0004-6766-3703 AD - Department of Neurosurgery, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China. LA - eng GR - 2024A1515110235/Guangdong Basic and Applied Basic Research Foundation/ GR - B2023414/Medical Scientific Research Foundation of Guangdong Province/ GR - YCTJ-2022-09/Talented Person Support Plan from The First Affiliated Hospital of Shantou University Medical College/ PT - Journal Article PL - England TA - Biol Cell JT - Biology of the cell JID - 8108529 RN - EC 3.5.1.98 (Histone Deacetylases) RN - EC 3.5.1.98 (Sin3 Histone Deacetylase and Corepressor Complex) RN - 0 (SIN3A transcription factor) RN - 0 (Repressor Proteins) SB - IM MH - Humans MH - *Glioma/metabolism/pathology/genetics MH - *Histone Deacetylases/metabolism MH - *Sin3 Histone Deacetylase and Corepressor Complex/metabolism MH - Cell Line, Tumor MH - Cell Proliferation MH - *Cell Differentiation MH - *Repressor Proteins/metabolism MH - *G1 Phase Cell Cycle Checkpoints MH - Cell Movement OTO - NOTNLM OT - FAM60A OT - G1/S arrest OT - HDAC OT - SIN3A OT - epigenetic regulation OT - glioma EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 08:34 PHST- 2026/08/24 00:00 [revised] PHST- 2026/05/09 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:34 [entrez] AID - e70088 [pii] AID - 10.1111/boc.70088 [doi] PST - ppublish SO - Biol Cell. 2026 Nov;118(11):e70088. doi: 10.1111/boc.70088. PMID- 42949187 OWN - NLM STAT- Publisher LR - 20261028 IS - 1526-4610 (Electronic) IS - 0017-8748 (Linking) DP - 2026 Oct 28 TI - Opioids as the earliest acute treatment for emergency department headache and short-term all-cause emergency department return: A two-center comparative cohort study. LID - 10.1111/head.70260 [doi] AB - OBJECTIVE: To compare first-line emergency department (ED) treatment classes for acute headache on short-term all-cause ED return. BACKGROUND: In-ED pain relief anchors acute headache trials but is recorded incompletely in routine care. All-cause ED return has not been used to compare first-line treatments; guidelines favor dopamine-receptor antagonists over opioids for acute migraine. METHODS: Retrospective two-center cohort of adults treated for headache in the ED (MIMIC-IV-ED, 2011-2019; MC-MED, 2020-2022); first-line treatment was the earliest qualifying acute agent. The primary contrast was opioids versus guideline-preferred dopamine-receptor antagonists. The primary outcome was 72-h all-cause ED return among discharged patients; 7-day return was secondary. Estimates, adjusted for prespecified confounders available in both databases, are reported as risk ratios (RRs) with 95% confidence intervals (CIs), pooled and site-specific. RESULTS: Among 13,285 treated adults (10,799 MIMIC-IV-ED; 2486 MC-MED), MIMIC-IV-ED opioid recipients were older than dopamine-antagonist recipients (median 48 vs. 39 years) and more often high-acuity at triage (980/1895 [51.7%] vs. 900/3056 [29.5%]). First-line opioids were associated with higher 72-h all-cause ED return (adjusted risks 6.8% vs. 3.8%; RR 1.78; 95% CI 1.31-2.32) and 7-day return (10.7% vs. 6.6%; RR 1.62; 95% CI 1.28-1.99). The MC-MED estimate was directionally concordant but anchors direction only (85 opioid-treated discharged patients). Restricting to a headache-coded or headache-triaged return attenuated the association, which was no longer statistically significant (72-h RR 1.31; 95% CI 0.91-1.88). CONCLUSION: Patients selected for first-line opioids were older and higher acuity and returned more often within 72 h and 7 days at both health systems. These risk-adjusted associations, conditional on discharge, reflect all-cause ED utilization rather than confirmed headache recurrence and do not establish that changing first-line class would change return rates. The attenuated headache-specific result is as consistent with administrative undercoding as with no association and is not a justification for prescribing opioids. CI - (c) 2026 American Headache Society. FAU - Gorenshtein, Alon AU - Gorenshtein A AUID- ORCID: 0009-0000-7542-8608 AD - Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA. AD - BRIDGE GenAI Lab, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA. FAU - Adiniaev, Yosef AU - Adiniaev Y AD - BRIDGE GenAI Lab, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA. FAU - Liba, Tom AU - Liba T AD - Azrieli Faculty of Medicine, Bar-Ilan University, Safed, Israel. FAU - Klang, Eyal AU - Klang E AD - BRIDGE GenAI Lab, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA. AD - Department of Radiology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA. FAU - Daniel, Oved AU - Daniel O AD - Neurology Division, Tel Aviv Sourasky University Medical Center, Tel Aviv, Israel. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Headache JT - Headache JID - 2985091R SB - IM OAB - Emergency departments use several medicines to treat headache, but pain scores during the visit are often missing or inconsistent, so less is known about what happens after patients go home. We used records from two large hospital systems to compare whether patients returned to the emergency department for any reason; patients whose first recorded treatment included an opioid returned within 72 h about 1.8 times as often as those given guideline-preferred dopamine-blocking medicines. Because patients treated with opioids were older and sicker at the start, this study does not show that opioids caused the return visits or that a different first medicine would prevent them, but the findings were similar across both hospital systems and add evidence to consider when choosing emergency headache treatment. OABL- eng OTO - NOTNLM OT - dopamine antagonists OT - emergency department OT - headache OT - migraine OT - opioids OT - return visit EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 08:33 PHST- 2026/09/16 00:00 [revised] PHST- 2026/07/15 00:00 [received] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:33 [entrez] AID - 10.1111/head.70260 [doi] PST - aheadofprint SO - Headache. 2026 Oct 28. doi: 10.1111/head.70260. PMID- 42949180 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1099-1344 (Electronic) IS - 0362-4803 (Linking) VI - 69 IP - 13-14 DP - 2026 Nov TI - Design, Synthesis, and Biological Evaluation of (99m)Tc-Labeled Thalidomide Analogs as Novel Tumor-Targeting Radiopharmaceuticals. LID - e70045 [pii] LID - 10.1002/jlcr.70045 [doi] AB - This study aimed to develop tumor-selective radiopharmaceuticals for noninvasive imaging using three thalidomide analogs: N-(2,6-dioxopiperidin-3-yl)-2-((3-ethyl-7-nitro-4-oxo-3,4-dihydroquinazolin-2-yl)thio)acetamide (DPDQA), 2-amino-N-(2,6-dioxopiperidin-3-yl)benzamide (ADPB), and 2-(2-chloroquinazolin-4-yl)hydrazine-1-carboxamide (CQHC). These compounds were radiolabeled with (99m)Tc via a direct labeling method using sodium dithionite (Na(2)S(2)O(4)) as a reducing agent. Optimal labeling conditions were determined by evaluating pH, ligand/reducing agent amounts, reaction time, and temperature. Radiochemical purity reached 92% +/- 0.31% for (99m)Tc-DPDQA (pH 8, 500-mug ligand, 500-mg Na(2)S(2)O(4), 15 min), 89.8% +/- 0.128% for (99m)Tc-ADPB (pH 7, 800-mug ligand, 25-mg Na(2)S(2)O(4), 15 min), and 92% +/- 0.16% for (99m)Tc-CQHC (pH 10, 200-mug ligand, 150-mg Na(2)S(2)O(4), 30 min). All complexes demonstrated good in vitro stability up to 24 h. In vivo biodistribution studies in tumor-bearing mice revealed high tumor uptake (T/NT ratios: 19.75, 6.18, and 22.72 for (99m)Tc-DPDQA, (99m)Tc-ADPB, and (99m)Tc-CQHC, respectively), with renal excretion as the primary clearance pathway. These results highlight the potential of (99m)Tc-labeled thalidomide analogs as selective tumor-imaging agents outperforming existing agents like (99m)Tc-TEPA. CI - (c) 2026 John Wiley & Sons Ltd. FAU - Rashed, Hassan M AU - Rashed HM AUID- ORCID: 0000-0003-0325-6434 AD - Labeled Compounds Department, Hot Laboratories and Waste Management Center, Egyptian Atomic Energy Authority, Cairo, Egypt. FAU - Mohamed, Ashraf Mohamed Hamed AU - Mohamed AMH AD - Radiation Protection Department, Nuclear and Radiological Regulatory Authority, Egyptian Atomic Energy Authority, Cairo, Egypt. FAU - Eissa, Ibrahim H AU - Eissa IH AD - Pharmaceutical Medicinal Chemistry and Drug Design Department, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt. FAU - Abdallah, Abdallah E AU - Abdallah AE AUID- ORCID: 0000-0003-2597-7419 AD - Pharmaceutical Medicinal Chemistry and Drug Design Department, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt. FAU - El-Zahabi, Mohamed Ayman AU - El-Zahabi MA AUID- ORCID: 0000-0003-4538-5840 AD - Pharmaceutical Medicinal Chemistry and Drug Design Department, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt. FAU - Sakr, Tamer M AU - Sakr TM AUID- ORCID: 0000-0001-9162-5687 AD - Radioactive Isotopes and Generator Department, Hot Laboratories and Waste Management Center, Egyptian Atomic Energy Authority, Cairo, Egypt. LA - eng PT - Journal Article PL - England TA - J Labelled Comp Radiopharm JT - Journal of labelled compounds & radiopharmaceuticals JID - 7610510 RN - 0 (Radiopharmaceuticals) RN - 4Z8R6ORS6L (Thalidomide) RN - 7440-26-8 (Technetium) RN - 0 (Organotechnetium Compounds) SB - IM MH - Animals MH - *Radiopharmaceuticals/chemical synthesis/pharmacokinetics/chemistry MH - Mice MH - *Thalidomide/analogs & derivatives/chemical synthesis/chemistry/pharmacokinetics MH - Isotope Labeling MH - Tissue Distribution MH - *Technetium/chemistry MH - *Drug Design MH - *Organotechnetium Compounds/chemical synthesis/pharmacokinetics/chemistry MH - Chemistry Techniques, Synthetic OTO - NOTNLM OT - SPECT OT - Technetium-99 m OT - radiolabeling OT - radiopharmaceuticals OT - thalidomide analogs OT - tumor imaging EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 08:33 PHST- 2026/08/28 00:00 [revised] PHST- 2026/07/01 00:00 [received] PHST- 2026/09/10 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:33 [entrez] AID - e70045 [pii] AID - 10.1002/jlcr.70045 [doi] PST - ppublish SO - J Labelled Comp Radiopharm. 2026 Nov;69(13-14):e70045. doi: 10.1002/jlcr.70045. PMID- 42949177 OWN - NLM STAT- Publisher LR - 20261028 IS - 1433-7347 (Electronic) IS - 0942-2056 (Linking) DP - 2026 Oct 28 TI - Arthroscopic labral repair versus debridement in non-athletic patients with femoroacetabular impingement: A prospective randomized study. LID - 10.1002/ksa.70661 [doi] AB - PURPOSE: To compare functional outcomes and pain scores between arthroscopic labral repair and selective labral debridement in a strictly defined non-athletic population with femoroacetabular impingement (FAI). METHODS: Between February 2024 and April 2025, 60 non-athletic patients (mean age 32.1 years) with symptomatic FAI were randomized to labral repair (n = 30) or debridement (n = 30). Non-athletic status was defined as individuals who engaged in non-competitive activity levels <3 h per week. All patients underwent concomitant cam/pincer resection as needed. The primary outcome was patient-reported clinical outcome, assessed using the Harris Hip Score (HHS), Hip Outcome Score-Activities of Daily Living (HOS-ADL) and Visual Analogue Scale (VAS) for pain, assessed preoperatively and postoperatively at 1, 3, 6 and 12 months. RESULTS: The repair group showed statistically superior HHS and HOS-ADL scores at 3 and 6 months postoperatively (p 11 000 covariates), with up to 5-year follow-up. We screened 181 prespecified incident outcomes across 15 body systems, pooling hazard ratios by random-effects meta-analysis. Robustness required leave-one-medical-center-out stability, E-value >/=1.5, and false discovery rate-adjusted P < .10. RESULTS: Among 70 982 matched adults, 118 robust associations were identified (65 for reduced risk and 53 for increased risk). Risk reductions were most consistent for cardiovascular outcomes, including myocarditis/cardiomyopathy (summary hazard ratio, 0.38 [95% CI, 0.32-0.44] vs SGLT2i), acute myocardial infarction (0.53 [0.42-0.66]), heart failure (0.71 [0.58-0.88] vs DPP4i), and thromboembolism (0.69-0.72), and for nephritis/nephrosis (0.73 [0.67-0.79]), nutritional anemia (0.70-0.81), coagulation disorders (0.73-0.81), and tobacco-related disorders (0.60 [0.49-0.74]). Risk increases were found for musculoskeletal conditions (22 associations); osteoarthritis 1.48 (1.29-1.68), stress fractures 1.68 (1.15-2.47), diverticular disease (1.18-1.33), sleep-wake disorders (1.27-1.54), and menopausal disorders (1.32-1.52). CONCLUSIONS: Glucagon-like peptide-1 receptor agonist initiation was associated with reduced cardiovascular, renal, hematologic, and tobacco-related risk, consistent with outcome trials. Increased risks for musculoskeletal, gastrointestinal, and sleep-wake disorders warrant monitoring as the clinical use of GLP-1RAs continues to expand. FAU - Vashisht, Rohit AU - Vashisht R AUID- ORCID: 0000-0002-2938-7259 AD - Bakar Computational Health Sciences Institute, University of California, San Francisco, USA. AD - Center for Data-driven Insights and Innovation, University of California Health, Oakland, USA. FAU - Dhruva, Sanket S AU - Dhruva SS AD - Section of Cardiology, Department of Medicine, School of Medicine, University of California, San Francisco, USA. AD - San Francisco Veterans Affairs Health Care System, CA, USA. FAU - Patel, Ayan AU - Patel A AD - Bakar Computational Health Sciences Institute, University of California, San Francisco, USA. AD - Center for Data-driven Insights and Innovation, University of California Health, Oakland, USA. FAU - Dahm, Lisa AU - Dahm L AD - Center for Data-driven Insights and Innovation, University of California Health, Oakland, USA. FAU - Morris, Pagan AU - Morris P AD - Center for Data-driven Insights and Innovation, University of California Health, Oakland, USA. FAU - Gonzalez, David AU - Gonzalez D AD - Center for Data-driven Insights and Innovation, University of California Health, Oakland, USA. FAU - Follett, Rob AU - Follett R AD - Center for Data-driven Insights and Innovation, University of California Health, Oakland, USA. FAU - Sirota, Marina AU - Sirota M AD - Bakar Computational Health Sciences Institute, University of California, San Francisco, USA. FAU - Singh, Karandeep AU - Singh K AD - Digital Health Innovation, University of California San Diego Health, USA. FAU - Koliwad, Suneil AU - Koliwad S AUID- ORCID: 0000-0002-7367-1054 AD - Division of Endocrinology and Metabolism, University of California, San Francisco, USA. FAU - Han, Cora AU - Han C AD - Center for Data-driven Insights and Innovation, University of California Health, Oakland, USA. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Diabetes Sci Technol JT - Journal of diabetes science and technology JID - 101306166 SB - IM PMC - PMC13716889 OTO - NOTNLM OT - GLP-1 receptor agonists OT - comparative effectiveness OT - electronic health records OT - target trial emulation OT - type 2 diabetes COIS- The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2027/10/28 CRDT- 2026/10/28 08:23 PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 08:23 [entrez] PHST- 2027/10/28 00:00 [pmc-release] AID - 10.1177_19322968261498914 [pii] AID - 10.1177/19322968261498914 [doi] PST - aheadofprint SO - J Diabetes Sci Technol. 2026 Oct 28:19322968261498914. doi: 10.1177/19322968261498914. PMID- 42949125 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2162-3279 (Electronic) VI - 16 IP - 11 DP - 2026 Nov TI - The Impact of Cladribine Tablets on Cognition Over One Year in Patients With Multiple Sclerosis: A Preliminary Study. LID - e71844 [pii] LID - 10.1002/brb3.71844 [doi] AB - INTRODUCTION: Cognitive impairment is common in multiple sclerosis (MS), affecting >40% of patients. Cladribine tablets are a high-efficacy MS disease-modifying therapy to prevent relapses and limit disease progression. Our previous study showed cortical gray matter lesion stability over one year after the start of cladribine, though its effect on cognition is not well known. This study aimed to investigate the one-year impact of cladribine tablets on cognition in multiple sclerosis. METHODS: Fourteen MS subjects [age (mean +/- SD) 50.2 +/- 10.8, 7.14% men, 57.1% relapsing-remitting MS (n = 8), 42.9% secondary progressive (n = 6), expanded disability status scale 4.1 +/- 2.1] were administered the Brief Repeatable Battery of Neuropsychological Tests (BRB-N) and the Stroop Color and Word Test (Stroop) at an average of 1.5 months from cladribine start. The BRB-N measures information processing speed (Symbol Digit Modalities Test, Paced Auditory Serial Addition Test [PASAT]), verbal memory (Selective Reminding Test [SRT]), visual-spatial memory (10/36 Spatial Recall Test), and verbal fluency (Word List Generation). Stroop assesses processing speed and executive function. When available, alternate versions of the individual cognitive tests were used at follow-up to reduce practice effects. Paired t-tests compared within-subject baseline and follow-up data. RESULTS: After one year, no worsening was noted on any cognitive test. Furthermore, both PASAT 3-second total correct and SRT total delayed recall trended toward improved scores on-study, with an average 3.4 (p = 0.07) and 1.1 (p = 0.05) point increase, respectively. CONCLUSION: In this preliminary study, no clear decline in cognitive performance was detectable over one year in patients newly started on cladribine tablets. CI - (c) 2026 The Author(s). Brain and Behavior published by Wiley Periodicals LLC. FAU - Callen, Alexis M AU - Callen AM AD - Brigham MS Center, Department of Neurology, Brigham and Women's Hospital/Mass General Brigham, Harvard Medical School, Boston, Massachusetts, USA. FAU - Glanz, Bonnie I AU - Glanz BI AD - Brigham MS Center, Department of Neurology, Brigham and Women's Hospital/Mass General Brigham, Harvard Medical School, Boston, Massachusetts, USA. FAU - Tauhid, Shahamat AU - Tauhid S AD - Brigham MS Center, Department of Neurology, Brigham and Women's Hospital/Mass General Brigham, Harvard Medical School, Boston, Massachusetts, USA. FAU - Healy, Brian C AU - Healy BC AD - Brigham MS Center, Department of Neurology, Brigham and Women's Hospital/Mass General Brigham, Harvard Medical School, Boston, Massachusetts, USA. FAU - Jinich, Ariele AU - Jinich A AD - Brigham MS Center, Department of Neurology, Brigham and Women's Hospital/Mass General Brigham, Harvard Medical School, Boston, Massachusetts, USA. FAU - Khalil, Samar AU - Khalil S AD - Brigham MS Center, Department of Neurology, Brigham and Women's Hospital/Mass General Brigham, Harvard Medical School, Boston, Massachusetts, USA. FAU - Houtchens, Maria K AU - Houtchens MK AD - Brigham MS Center, Department of Neurology, Brigham and Women's Hospital/Mass General Brigham, Harvard Medical School, Boston, Massachusetts, USA. FAU - Mateen, Farrah J AU - Mateen FJ AD - Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA. FAU - Napoli, Salvatore AU - Napoli S AD - Neurology Center of New England, Foxborough, Massachusetts, USA. FAU - Rizvi, Syed AU - Rizvi S AD - Multiple Sclerosis Center of Rhode Island, Warren Alpert Medical School of Brown University, Providence, Rhode Island, USA. FAU - Singhal, Tarun AU - Singhal T AD - Brigham MS Center, Department of Neurology, Brigham and Women's Hospital/Mass General Brigham, Harvard Medical School, Boston, Massachusetts, USA. FAU - Zurawski, Jonathan AU - Zurawski J AD - Brigham MS Center, Department of Neurology, Brigham and Women's Hospital/Mass General Brigham, Harvard Medical School, Boston, Massachusetts, USA. FAU - Bakshi, Rohit AU - Bakshi R AD - Brigham MS Center, Department of Neurology, Brigham and Women's Hospital/Mass General Brigham, Harvard Medical School, Boston, Massachusetts, USA. LA - eng GR - EMD Serono/ PT - Journal Article PL - United States TA - Brain Behav JT - Brain and behavior JID - 101570837 RN - 47M74X9YT5 (Cladribine) RN - 0 (Immunosuppressive Agents) RN - 0 (Tablets) SB - IM MH - Humans MH - *Cladribine/administration & dosage/pharmacology/therapeutic use MH - Male MH - Female MH - Middle Aged MH - Adult MH - *Immunosuppressive Agents/administration & dosage/pharmacology/therapeutic use MH - *Cognition/drug effects MH - *Multiple Sclerosis/drug therapy/complications/psychology MH - Neuropsychological Tests MH - Multiple Sclerosis, Relapsing-Remitting/drug therapy MH - Tablets MH - *Cognitive Dysfunction/drug therapy/etiology PMC - PMC13715071 OTO - NOTNLM OT - brain OT - cladribine OT - cognition OT - cognitive testing OT - multiple sclerosis OT - treatment COIS- Dr. Healy has received research support from Novartis and Sanofi/Genzyme. Dr. Houtchens has received consulting honoraria from Genentech, EMD Serono, Novartis, and TG therapeutics, and research support from Biogen, Novartis, and Roche/Genentech. Dr. Mateen has received research funding from Bristol-Myers Squibb, EMD Serono, Genentech, Horizon Therapeutics, Novartis, and TG Therapeutics, and has consulted for Alexion, EMD Serono, Genentech, and Horizon Therapeutics. Dr. Napoli has received speaking honoraria from EMD Serono, Biogen, Eisai, TG Therapeutics, and Bristol Myers Squibb. Dr. Rizvi has received advisory board consulting fees from Amgen and TG Therapeutics. Dr. Singhal has received research support from Novartis, EMD Serono, and Genzyme-Sanofi, payment for consulting from Novartis, Genentech, EMD Serono, Genzyme-Sanofi, TG Therapeutics, Eisai, and Biogen, and speaking fees from Tiziana Life Sciences. Dr. Zurawski has received research support from Novartis and I-Mab Biopharma. Dr. Bakshi has received speaking honoraria from EMD Serono, advisory board consulting fees from Sanofi, and research support from Novartis. The remaining authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/28 CRDT- 2026/10/28 08:16 PHST- 2026/09/29 00:00 [revised] PHST- 2026/07/15 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:16 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e71844 [pii] AID - BRB371844 [pii] AID - 10.1002/brb3.71844 [doi] PST - ppublish SO - Brain Behav. 2026 Nov;16(11):e71844. doi: 10.1002/brb3.71844. PMID- 42949117 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1940-087X (Electronic) IS - 1940-087X (Linking) IP - 236 DP - 2026 Oct 27 TI - Microvascular Obstruction After Reperfusion in ST-Segment Elevation Myocardial Infarction: A Structured Narrative Review. LID - 10.3791/74890 [doi] AB - Primary percutaneous coronary intervention (pPCI) restores epicardial coronary flow in ST-segment elevation myocardial infarction (STEMI), but microvascular and tissue-level reperfusion may remain impaired. Cardiovascular magnetic resonance (CMR)-defined microvascular obstruction (MVO) and intramyocardial hemorrhage (IMH) both mark severe reperfusion injury but reflect distinct tissue processes. This structured narrative review synthesizes evidence on MVO mechanisms, multimodal phenotypes, and interventions. Available evidence implicates interacting contributions from distal embolization, endothelial barrier and vasomotor dysfunction, pericyte-mediated capillary constriction, immunothrombosis, and tissue edema. IMH reflects more extensive microvascular destruction with erythrocyte extravasation. Angiography, electrocardiography, invasive physiology, angiography-derived indices, echocardiography, and CMR assess different pathophysiologic levels. Discordance between modalities is therefore not necessarily contradictory. Across trials, improvements in surrogate endpoints have often not translated into long-term clinical benefit, partly because of mechanistic heterogeneity and misaligned treatment timing and endpoints. A conceptual, reperfusion-centered T- to T5 research framework is proposed for serial within-patient assessment. It links early physiology, inflammatory and thrombotic biomarkers, acute-phase CMR, and long-term remodeling. Future trials should align mechanistic enrichment, intervention timing, and endpoint selection to establish a continuous evidence chain from target engagement to clinical outcomes. FAU - Long, Xiaoyan AU - Long X AD - Beijing University of Chinese Medicine. FAU - Lin, Qian AU - Lin Q AD - Department of Cardiology, Dongzhimen Hospital, Beijing University of Chinese Medicine. FAU - Li, Peng AU - Li P AD - Beijing University of Chinese Medicine. FAU - Ren, Xueyu AU - Ren X AD - Beijing University of Chinese Medicine. FAU - Lin, Hui AU - Lin H AD - Beijing University of Chinese Medicine. FAU - Zhang, Piao AU - Zhang P AD - Beijing University of Chinese Medicine. FAU - Yuan, Hangtao AU - Yuan H AD - Beijing University of Chinese Medicine. FAU - Cui, Xiaoyun AU - Cui X AD - Department of Cardiology, Dongfang Hospital, Beijing University of Chinese Medicine; yun6171@126.com. LA - eng PT - Journal Article PT - Review PT - Video-Audio Media DEP - 20261027 PL - United States TA - J Vis Exp JT - Journal of visualized experiments : JoVE JID - 101313252 SB - IM MH - Humans MH - *ST Elevation Myocardial Infarction/surgery/physiopathology MH - *Myocardial Reperfusion/adverse effects/methods MH - Animals MH - *Myocardial Reperfusion Injury/physiopathology/pathology MH - Percutaneous Coronary Intervention/methods MH - *Microvessels/pathology/physiopathology MH - *Myocardial Infarction/physiopathology EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 08:15 PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:15 [entrez] AID - 10.3791/74890 [doi] PST - epublish SO - J Vis Exp. 2026 Oct 27;(236). doi: 10.3791/74890. PMID- 42949116 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1940-087X (Electronic) IS - 1940-087X (Linking) IP - 236 DP - 2026 Oct 27 TI - Marker-Free Chromosomal Integration of a Multigene Cassette in Escherichia coli for Heavy-Metal Bioremediation Development. LID - 10.3791/73609 [doi] AB - Heavy metal contamination remains a significant environmental challenge due to the persistence, toxicity, and bioaccumulation of metal pollutants. Genetically engineered bacteria offer a potential approach to heavy metal remediation; however, plasmid-based expression systems can present limitations associated with plasmid maintenance and antibiotic selection. This study describes a protocol for constructing a marker-free, multigene recombinant E. coli strain designed for the chromosomal integration of genes associated with heavy-metal resistance and sequestration. A 4,741 bp multigene cassette comprising cadD, cadA, and metallothionein (mt), together with a lipoprotein outer membrane protein A (Lpp-OmpA) surface display system and a green fluorescent protein (gfp) reporter gene under the control of the constitutive pyruvate dehydrogenase (Ppdh) promoter, was designed and synthesized. The construct was cloned into the pKIKOArsBKm integration vector and introduced into E. coli MG1655 using lambda (lambda) Red-mediated recombination. The kanamycin resistance cassette was subsequently excised using the flippase-flippase recognition target (FLP-FRT) recombination system. Construct assembly and chromosomal integration were evaluated by restriction enzyme analysis, plasmid isolation, and polymerase chain reaction (PCR) amplification, while loss of antibiotic resistance was assessed by antibiotic sensitivity testing. Amplification of the expected cadD, cadA, and mt fragments from genomic DNA, together with the absence of detectable plasmid DNA, provided evidence consistent with chromosomal integration of the multigene cassette. Although this protocol establishes a framework for constructing and molecularly evaluating the recombinant strain, definitive confirmation of the integration locus, functional gene expression, long-term genetic stability, and heavy metal biosorption performance requires further experimental validation. FAU - Dissanayake, Dilani AU - Dissanayake D AD - Biotechnology Laboratory, Department of Chemistry, Faculty of Science, University of Colombo; dilanichathuri93@gmail.com. FAU - Chandrasekharan, Naduviladath Vishvanath AU - Chandrasekharan NV AD - Biotechnology Laboratory, Department of Chemistry, Faculty of Science, University of Colombo. FAU - Wachi, Masaaki AU - Wachi M AD - Department of Life Science and Technology, Institute of Science Tokyo. FAU - Wijayarathna, Champika Dilrukshi AU - Wijayarathna CD AD - Biotechnology Laboratory, Department of Chemistry, Faculty of Science, University of Colombo. LA - eng PT - Journal Article PT - Video-Audio Media DEP - 20261027 PL - United States TA - J Vis Exp JT - Journal of visualized experiments : JoVE JID - 101313252 RN - 0 (Metals, Heavy) RN - 147336-22-9 (Green Fluorescent Proteins) SB - IM MH - *Escherichia coli/genetics/metabolism MH - *Metals, Heavy/metabolism MH - Chromosomes, Bacterial/genetics MH - Plasmids/genetics MH - Green Fluorescent Proteins/genetics/biosynthesis EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 08:15 PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:15 [entrez] AID - 10.3791/73609 [doi] PST - epublish SO - J Vis Exp. 2026 Oct 27;(236). doi: 10.3791/73609. PMID- 42949115 OWN - NLM STAT- Publisher LR - 20261028 IS - 1540-8159 (Electronic) IS - 0147-8389 (Linking) DP - 2026 Oct 28 TI - Left Atrial Appendage Occlusion Versus Oral Anticoagulation in Atrial Fibrillation: A Comprehensive Evidence Synthesis. LID - 10.1111/pace.70476 [doi] AB - BACKGROUND: Oral anticoagulation (OAC) remains the cornerstone of stroke prevention in atrial fibrillation (AF) but is limited by bleeding risk. Left atrial appendage occlusion (LAAO) devices offer an alternative; however, recent randomized trials have reported inconsistent efficacy compared with OAC. METHODS: We conducted a systematic review and meta-analysis of randomized controlled trials (RCTs) comparing LAAO with OAC through April 1, 2026, using MEDLINE, EMBASE, and Cochrane CENTRAL. Eligible studies enrolled adults (>/=18 years) with AF randomized to LAAO or OAC. Primary and secondary outcomes were pooled as risk ratios (RR) with 95% confidence intervals (CIs). Individual patient-level data (IPD) were reconstructed from published Kaplan-Meier curves for time-to-event sensitivity analyses. RESULTS: Six RCTs (n = 7,004; mean age 72 years; CHA(2)DS(2)‑VASc 3.9; HAS‑BLED 1.7; mean follow-up 38 months) were included. LAAO showed no significant difference in ischemic stroke (RR 1.20, 95% CI 0.82-1.76), major bleeding (RR 0.77, 95% CI 0.50-1.17), or all-cause mortality (RR 0.96, 95% CI 0.82-1.13) compared with OAC. Hemorrhagic stroke risk was significantly lower with LAAO (RR 0.53, 95% CI 0.30-0.93). Reconstructed IPD demonstrated similar stroke and bleeding risks (HR 1.24 and 0.84, respectively) but lower all-cause mortality with LAAO (HR 0.70, 95% CI 0.52-0.95). CONCLUSION: LAAO provides comparable ischemic stroke prevention and major bleeding outcomes to OAC and reduces hemorrhagic stroke risk. Reconstructed IPD analyses suggest a possible mortality benefit that warrants further investigation. Future studies using contemporary devices and post-ablation populations are needed to refine patient selection and further characterize the comparative clinical outcomes of LAAO and OAC in AF patients. CI - (c) 2026 Wiley Periodicals LLC. FAU - Bashir, Zubair AU - Bashir Z AUID- ORCID: 0000-0002-1821-6622 AD - University of Texas Medical Branch, Galveston, Texas, USA. FAU - Rehman, Muhammad Abdul AU - Rehman MA AUID- ORCID: 0000-0003-3540-0998 AD - Department of Internal Medicine, University of Central Florida, Tallahassee, Florida, USA. AD - Mayo Clinic School of Graduate Medical Education, Mayo Clinic, Rochester, Minnesota, USA. FAU - Hamza, Mohammad AU - Hamza M AD - Department of Internal Medicine, Tidal Health, Salisbury, Maryland, USA. FAU - Yang, Michelle AU - Yang M AD - University of Texas Medical Branch, Galveston, Texas, USA. FAU - Butt, Mohammad Omer AU - Butt MO AUID- ORCID: 0009-0008-4008-8912 AD - Department of Internal Medicine, Memorial Satilla Health, Waycross, Georgia, USA. FAU - Bukhari, Syed AU - Bukhari S AD - Division of Cardiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Pacing Clin Electrophysiol JT - Pacing and clinical electrophysiology : PACE JID - 7803944 SB - IM OTO - NOTNLM OT - Atrial fibrillation OT - left atrial appendage occlusion device OT - major bleeding OT - mortality OT - oral anticoagulants OT - stroke EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 08:15 PHST- 2026/10/07 00:00 [revised] PHST- 2026/07/30 00:00 [received] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:15 [entrez] AID - 10.1111/pace.70476 [doi] PST - aheadofprint SO - Pacing Clin Electrophysiol. 2026 Oct 28. doi: 10.1111/pace.70476. PMID- 42949109 OWN - NLM STAT- Publisher LR - 20261028 IS - 1460-2423 (Electronic) IS - 0959-6658 (Linking) DP - 2026 Oct 28 TI - How the terminal glucoside of the N-glycan donor affects the catalytic efficiency of the eukaryotic oligosaccharyltransferase. LID - cwag102 [pii] LID - 10.1093/glycob/cwag102 [doi] AB - The eukaryotic oligosaccharyltransferase (OST) is responsible for initiating N-glycosylation of secreted proteins by transferring a pre-assembled lipid-linked oligosaccharide (LLO) donor to target asparagine residues most often found within N-x-S/T consensus sequences, or sequons. OST preferentially selects LLO donors with a distinctive glucoside Glc-alpha(1-2)-Glc-alpha(1-3)-Glc-alpha(1-3)- capping the A-branch. This motif is cleaved right after the N-glycan transfer in a stepwise manner, before the folded glycoprotein exits the endoplasmic reticulum quality control (ERQC) cycle. While the -Glc-alpha(1-3)-Glc-alpha(1-3)- disaccharide regulates access and binding to the calreticulin/calnexin chaperones, the terminal Glc-alpha(1-2)- is removed immediately after OST catalysis, suggesting that its biological function may be directly linked to the OST catalytic efficiency. To understand how and why this capping motif affects the OST N-glycosylation efficiency, we rebuilt 3D models of the yeast OST in complex with an acceptor peptide and LLO substrates with and without terminal Glc-alpha(1-2)- and analysed their stability with all-atom molecular dynamics (MD) simulations through conventional and Gaussian-accelerated (GaMD) sampling schemes. Our results indicate that the terminal Glc-alpha(1-2)- is essential to anchor the full-length LLO donor to the OST through a complex network of intermolecular contacts extending from the catalytic site to distal subdomains. We show how this contact network is crucial to preserve the LLO catalytically productive alignment of its reducing end. We also show that the removal of the terminal Glc-alpha(1-2)- leads to an increased flexibility of the LLO, which displaces the reducing end, leading to less catalytically productive states. CI - (c) The Author(s) 2026. Published by Oxford University Press. FAU - Tropea, Beatrice AU - Tropea B AD - Hamilton Institute, Maynooth University, Maynooth, Ireland. FAU - Fadda, Elisa AU - Fadda E AUID- ORCID: 0000-0002-2898-7770 AD - School of Biological Sciences, University of Southampton, Southampton, United Kingdom. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Glycobiology JT - Glycobiology JID - 9104124 SB - IM OTO - NOTNLM OT - Glucoside OT - LLO OT - Molecular Dynamics OT - N-glycosylation OT - OST EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 08:15 PHST- 2026/08/07 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:15 [entrez] AID - 9003596 [pii] AID - 10.1093/glycob/cwag102 [doi] PST - aheadofprint SO - Glycobiology. 2026 Oct 28:cwag102. doi: 10.1093/glycob/cwag102. PMID- 42949107 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1940-087X (Electronic) IS - 1940-087X (Linking) IP - 236 DP - 2026 Oct 27 TI - Non-Invasive Neuromodulation for Post-Stroke Anxiety Symptoms: An Umbrella Review and Evidence Mapping of Systematic Reviews and Meta-Analyses. LID - 10.3791/73132 [doi] AB - Post-stroke anxiety can impair rehabilitation, participation, and quality of life. This umbrella review mapped systematic reviews on non-invasive neuromodulation for anxiety symptoms after stroke. PubMed, Web of Science, the Cochrane Library, and Embase were searched from inception through June 1, 2026. Published reviews with quantitative anxiety results were eligible, including reviews in which anxiety was secondary to pain, sleep disturbance, or depression. Reporting completeness, methodological quality, and certainty of evidence were assessed using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 statement, A MeaSurement Tool to Assess systematic Reviews 2 (AMSTAR 2), and the Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach, respectively. Seven reviews were mapped, comprising six pairwise meta-analyses and one Bayesian network meta-analysis. Selected estimates suggested symptom improvement, whereas others showed no clear difference. All reviews had low or critically low methodological quality. Interpretation was limited by overlapping trials, small outcome-specific sample sizes, heterogeneous interventions, and inconsistencies across source reports. Anxiety-score changes in populations recruited for other post-stroke conditions do not establish a direct effect on clinically defined post-stroke anxiety. Source-level population eligibility and certainty judgments require particular scrutiny when mixed neurological populations contribute to pooled estimates. Non-invasive neuromodulation remains investigational for post-stroke anxiety. Current evidence does not support routine use of a specific protocol or replacement of standard multidisciplinary stroke care. Future trials should prospectively define anxiety, standardize stimulation and sham procedures, and assess sustained benefit and safety. FAU - Li, Tiantian AU - Li T AD - Geriatric Rehabilitation Department, Hunan Rehabilitation Hospital. FAU - He, Lijuan AU - He L AD - Geriatric Rehabilitation Department, Hunan Rehabilitation Hospital. FAU - Li, Fenghui AU - Li F AD - Geriatric Rehabilitation Department, Hunan Rehabilitation Hospital. FAU - Zhang, Jianyun AU - Zhang J AD - Geriatric Rehabilitation Department, Hunan Rehabilitation Hospital; 15874256166@163.com. LA - eng PT - Journal Article PT - Systematic Review PT - Video-Audio Media DEP - 20261027 PL - United States TA - J Vis Exp JT - Journal of visualized experiments : JoVE JID - 101313252 SB - IM MH - Humans MH - *Stroke/complications/psychology MH - *Anxiety/therapy/etiology MH - Systematic Reviews as Topic MH - Meta-Analysis as Topic EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 08:15 PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:15 [entrez] AID - 10.3791/73132 [doi] PST - epublish SO - J Vis Exp. 2026 Oct 27;(236). doi: 10.3791/73132. PMID- 42949093 OWN - NLM STAT- Publisher LR - 20261028 IS - 1462-0332 (Electronic) IS - 1462-0324 (Linking) DP - 2026 Oct 28 TI - Longitudinal ultrasound assesment of skeletal muscle in systemic sclerosis: association with disease activity and severity. LID - keag601 [pii] LID - 10.1093/rheumatology/keag601 [doi] AB - OBJECTIVES: Systemic sclerosis (SSc) is an autoimmune fibroinflammatory disease that may predispose to the development of muscle loss through chronic inflammation and functional limitation. Longitudinal assessment using ultrasound provides insights into muscle loss and its association with disease activity and severity. This study sought to evaluate early muscle loss using ultrasound in SSc patients and investigate their relationship with disease activity and severity. METHODS: A longitudinal study was conducted in 52 SSc patients, with muscle thickness at various sites, including biceps brachii, triceps brachii, medial gastrocnemius, and the rectus femoris measured at 0, 6, and 12. months using ultrasound. Associations with disease activity, Medsger's Severity Score and modified Rodnan skin score (mRSS) were analyzed. The diagnostic value of changes in muscle thickness for determining disease activity was assessed using ROC curve analysis. RESULTS: A significant inverse correlation was identified between the change in gastrocnemius muscle thickness and disease activity over the initial 6-month period (r=-0.36, p = 0.026). A significant relationship was found between the difference in mRSS and the difference in biceps brachii muscle thickness between baseline and 6 months (r=-0.33, p < 0.05). Decreasing muscle thickness paralleled increasing severity scores. ROC analysis for gastrocnemius muscle indicated moderate discriminative performance for detecting clinically relevant muscle changes. CONCLUSION: Longitudinal ultrasound assessment effectively reflects progressive reduction in muscle thickness in SSc, correlating with disease activity and severity. These findings highlight ultrasound as a practical, non-invasive tool for early detection and monitoring, potentially capturing subclinical changes, specifically a decrease in muscle thickness, in SSc. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Agbuga, Ozlem Dogan AU - Agbuga OD AUID- ORCID: 0000-0002-4998-9177 AD - Department of Rheumatology, Osmaniye Training and Research Hospital, Osmaniye, Turkey. FAU - Kose, Sevgul AU - Kose S AUID- ORCID: 0000-0003-2095-9449 AD - Department of Radiology, Cukurova University Faculty of Medicine, Adana, Turkey. FAU - Pehlivan, Umur Anil AU - Pehlivan UA AUID- ORCID: 0000-0001-5871-0695 AD - Department of Radiology, Baskent University Faculty of Medicine, Dr.Turgut Noyan Application and Research Center, Adana, Turkey. FAU - Arslan, Yusuf Kemal AU - Arslan YK AUID- ORCID: 0000-0003-1308-8569 AD - Department of Biostatistics, Cukurova University Faculty of Medicine, Adana, Turkey. FAU - Arslan, Didem AU - Arslan D AUID- ORCID: 0000-0002-9654-2183 AD - Department of Internal Medicine, Division of Rheumatology, Cukurova University Faculty of Medicine, Adana, Turkey. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Rheumatology (Oxford) JT - Rheumatology (Oxford, England) JID - 100883501 SB - IM OTO - NOTNLM OT - Systemic sclerosis OT - biceps brachii OT - gastrocnemius OT - muscle ultrasonography EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 08:14 PHST- 2026/03/22 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:14 [entrez] AID - 9003578 [pii] AID - 10.1093/rheumatology/keag601 [doi] PST - aheadofprint SO - Rheumatology (Oxford). 2026 Oct 28:keag601. doi: 10.1093/rheumatology/keag601. PMID- 42949088 OWN - NLM STAT- Publisher LR - 20261028 IS - 1741-7899 (Electronic) IS - 1470-1626 (Linking) DP - 2026 Oct 28 TI - Effects of maternal exercise intensity during pregnancy on fetal ovarian development in mice. LID - xaag135 [pii] LID - 10.1093/reprod/xaag135 [doi] AB - Maternal exercise is widely regarded as beneficial for metabolic homeostasis and pregnancy outcomes; however, whether different exercise intensities are associated with distinct patterns of fetal ovarian development remains unclear. Pregnant C57BL/6J mice were assigned to control, limited exercise (LE; cage-space restriction from GD0 to GD17), moderate exercise (ME; 10 m/min treadmill running from GD9 to GD17), excessive exercise (EE; 14 m/min treadmill running from GD9 to GD17), or limited exercise with moderate exercise superimposed from GD9 to GD17 (LE&ME). The litter was used as the biological unit. Fetal ovaries collected on GD18 were assessed for morphology, proliferation and apoptosis, estradiol (E2) content, steroidogenesis-related markers, oocyte-development markers and candidate signalling pathways. Compared with controls, LE had lower ovarian volume, cumulative oocyte-profile counts, proliferative and oocyte-development indices, together with higher apoptosis-related indices, ovarian E2 content and steroidogenesis-related markers. ME increased ovarian volume, cumulative oocyte-profile counts, proliferative activity, E2 content, and several oocyte- and steroidogenesis-related markers. EE increased E2 and steroidogenesis-related markers but reduced oocyte-development markers without parallel increases in the measured morphometric or proliferative indices. LE&ME showed lower ovarian E2 content than LE and partially attenuated several LE-associated changes in proliferation, apoptosis and oocyte-development markers. Exploratory analyses identified correlations between Igf1 or Mek1 expression and selected developmental markers. Collectively, different maternal exercise intensities were associated with distinct fetal ovarian developmental profiles, with ME showing a more favourable overall profile. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Society for Reproduction and Fertility. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Mo, Fan AU - Mo F AD - Department of Orthopaedic Surgery, Division of Joint Surgery and Sports Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. FAU - Chen, Lu AU - Chen L AD - Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan 430071, China. FAU - Huang, Jing AU - Huang J AD - Department of Otorhinolaryngology Head and Neck Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. FAU - Wang, Zhaojun AU - Wang Z AD - Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan 430071, China. FAU - Yin, Mengying AU - Yin M AD - Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan 430071, China. FAU - Chen, Liaobin AU - Chen L AD - Department of Orthopaedic Surgery, Division of Joint Surgery and Sports Medicine, Zhongnan Hospital of Wuhan University, Wuhan 430071, China. AD - Hubei Provincial Key Laboratory of Developmentally Originated Diseases, Wuhan 430071, China. FAU - Wang, Hui AU - Wang H AD - Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan 430071, China. AD - Hubei Provincial Key Laboratory of Developmentally Originated Diseases, Wuhan 430071, China. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Reproduction JT - Reproduction (Cambridge, England) JID - 100966036 SB - IM OTO - NOTNLM OT - IGF1/MAPK OT - exercise intensity OT - ovarian development OT - pregnancy exercise EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 08:13 PHST- 2026/06/11 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:13 [entrez] AID - 9003591 [pii] AID - 10.1093/reprod/xaag135 [doi] PST - aheadofprint SO - Reproduction. 2026 Oct 28:xaag135. doi: 10.1093/reprod/xaag135. PMID- 42949087 OWN - NLM STAT- Publisher LR - 20261028 IS - 1531-8257 (Electronic) IS - 0885-3185 (Linking) DP - 2026 Oct 28 TI - Neuromelanin-Sensitive MRI as a Marker of Clinical Severity and Disease Burden in Parkinson's Disease: A Systematic Review and Meta-analysis. LID - 10.1002/mds.70588 [doi] AB - The neuropathology of Parkinson's disease (PD) is characterized in part by the degeneration of catecholaminergic neurons, particularly dopaminergic neurons in the substantia nigra pars compacta (SNpc) and noradrenergic neurons in the locus coeruleus (LC), yet no validated in vivo biomarker quantifies this process. Neuromelanin-sensitive magnetic resonance imaging (NM-MRI) enables noninvasive assessment of these neuronal populations, although relationships with clinical severity, disease burden, and progression remain incompletely defined. We conducted the first Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA)-guided systematic review and random-effects meta-analysis examining associations between NM-MRI measures and clinical outcomes in PD, including subgroup, meta-regression, and sensitivity analyses. Reduced NM-MRI measures were associated with greater motor impairment (18 studies; pooled r = -0.406), longer disease duration (14; r = -0.426), higher Hoehn and Yahr stage (10; r = -0.336), and higher levodopa equivalent daily dose (6; r = -0.337) (all P < 0.001). Thirty-nine studies (1983 patients with PD) were included in the systematic review. Narrative synthesis demonstrated distinct SNpc/LC associations: SNpc measures were primarily associated with motor dysfunction and disease stage, displaying hemispheric asymmetry, intranigral topography, and phenotype-specific patterns, whereas LC measures were preferentially linked to cognitive, affective, autonomic, and sleep-related symptoms. Four longitudinal studies (~1.5-3 years) reported declines in selected NM-MRI measures (~5%-19% annually). Multimodal studies suggested NM-MRI captures a complementary dimension of nigrostriatal pathology alongside dopaminergic, iron-sensitive, and diffusion-based markers. NM-MRI provides a biologically interpretable in vivo measure of catecholaminergic neuron integrity and survival in PD, with consistent cross-sectional associations with clinical disease burden. Emerging longitudinal and multimodal evidence supports its potential as a progression-sensitive imaging biomarker for disease-modifying clinical trials. (c) 2026 The Author(s). Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society. CI - (c) 2026 The Author(s). Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society. FAU - Akande, Rayo AU - Akande R AUID- ORCID: 0000-0003-4858-1679 AD - Roche Pharma Research and Early Development, Neuroscience and Rare Diseases Discovery and Translational Area, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd, Basel, Switzerland. FAU - Kustermann, Thomas AU - Kustermann T AD - Roche Pharma Research and Early Development, Neuroscience and Rare Diseases Discovery and Translational Area, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd, Basel, Switzerland. FAU - Holiga, Stefan AU - Holiga S AD - Roche Pharma Research and Early Development, Neuroscience and Rare Diseases Discovery and Translational Area, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd, Basel, Switzerland. FAU - Anzures-Cabrera, Judith AU - Anzures-Cabrera J AD - Roche Products Ltd, Welwyn Garden City, United Kingdom. FAU - Brundin, Patrik AU - Brundin P AD - Roche Pharma Research and Early Development, Neuroscience and Rare Diseases Discovery and Translational Area, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd, Basel, Switzerland. FAU - Pagano, Gennaro AU - Pagano G AUID- ORCID: 0000-0002-6247-8988 AD - Roche Pharma Research and Early Development, Neuroscience and Rare Diseases Discovery and Translational Area, Roche Innovation Center Basel, F. Hoffmann-La Roche Ltd, Basel, Switzerland. AD - University of Exeter Medical School, London, United Kingdom. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Mov Disord JT - Movement disorders : official journal of the Movement Disorder Society JID - 8610688 SB - IM OTO - NOTNLM OT - Parkinson's disease OT - imaging biomarker OT - locus coeruleus OT - neuromelanin-sensitive MRI OT - substantia nigra EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 08:13 PHST- 2026/09/26 00:00 [revised] PHST- 2026/05/24 00:00 [received] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:13 [entrez] AID - 10.1002/mds.70588 [doi] PST - aheadofprint SO - Mov Disord. 2026 Oct 28. doi: 10.1002/mds.70588. PMID- 42949086 OWN - NLM STAT- Publisher LR - 20261028 IS - 1462-0332 (Electronic) IS - 1462-0324 (Linking) DP - 2026 Oct 28 TI - Conductive and sensorineural hearing impairment in juvenile idiopathic arthritis: a systematic review and meta-analysis. LID - keag602 [pii] LID - 10.1093/rheumatology/keag602 [doi] AB - OBJECTIVES: To determine the prevalence of ossicular stiffness and cochlear dysfunction in patients with juvenile idiopathic arthritis (JIA) compared with healthy controls. METHODS: We searched PubMed (1966-27 December 2025), Embase (1947-27 December 2025), and the Cochrane Central Register of Controlled Trials (1996-27 December 2025). Studies of patients diagnosed with JIA who underwent audiological examinations (cochlear and middle ear function) were selected for the review. Random-effects models were used to calculate pooled relative risks (RR) and standardized mean differences (SMD). RESULTS: Seven studies (237 patients with JIA; 132 healthy controls) were included; six were judged high methodological quality (Newcastle-Ottawa score >/= 8). Children with JIA exhibited a markedly higher prevalence of type As tympanograms (RR = 13.93, 95% CI 5.83-33.27) and elevated pure-tone hearing thresholds, with the largest effect observed at the extended high frequency of 16 kHz (SMD = 1.52, 95% CI 1.04-1.99). Pooled otoacoustic emission amplitudes trended lower in JIA but did not reach statistical significance (SMD = -1.72, 95% CI - 8.77 to 5.33; I(2) = 96%), although the single study reporting DPOAE suggested a marked reduction. CONCLUSION: JIA is associated with a quantifiable dual-compartment audiological burden-a markedly elevated risk of mechanical middle-ear stiffness and a frequency-dependent sensorineural deficit most pronounced beyond the conventional audiometric range. Tympanometry and extended high-frequency audiometry may provide complementary information for detecting subclinical auditory involvement in JIA. The potential role of OAEs, including DPOAE, requires confirmation in larger prospective studies. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Chen, Shih-Huan AU - Chen SH AD - Department of Education, China Medical University Hospital, Taichung 404332, Taiwan. FAU - Lee, Yung-Hsuan AU - Lee YH AD - Department of Education, China Medical University Hospital, Taichung 404332, Taiwan. FAU - Wang, Ching-Yuan AU - Wang CY AD - School of Medicine, College of Medicine, China Medical University, Taichung 40402, Taiwan. AD - Department of Otorhinolaryngology-Head and Neck Surgery, China Medical University Hospital, Taichung 404332, Taiwan. FAU - Wang, Tang-Chuan AU - Wang TC AD - School of Medicine, College of Medicine, China Medical University, Taichung 40402, Taiwan. AD - Department of Otorhinolaryngology-Head and Neck Surgery, China Medical University Hsinchu Hospital, Hsinchu, Taiwan. AD - Department of Master Program for Biomedical Engineering, College of Biomedical Engineering, China Medical University, Taichung, Taiwan. FAU - Lin, Fang-Wei AU - Lin FW AD - Department of Education, China Medical University Hospital, Taichung 404332, Taiwan. FAU - Lin, Chia-Der AU - Lin CD AD - School of Medicine, College of Medicine, China Medical University, Taichung 40402, Taiwan. AD - Department of Otorhinolaryngology-Head and Neck Surgery, China Medical University Hospital, Taichung 404332, Taiwan. FAU - Wu, Po-Chang AU - Wu PC AD - School of Medicine, College of Medicine, China Medical University, Taichung 40402, Taiwan. AD - Division of Rheumatology and Immunology, Department of Internal Medicine, China Medical University Hospital, Taichung 404332, Taiwan. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Rheumatology (Oxford) JT - Rheumatology (Oxford, England) JID - 100883501 SB - IM OTO - NOTNLM OT - DPOAE OT - Hearing loss OT - Juvenile idiopathic arthritis OT - Tympanograms OT - pure-tone audiometric thresholds (PTA) EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 08:13 PHST- 2026/05/13 00:00 [received] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:13 [entrez] AID - 9003583 [pii] AID - 10.1093/rheumatology/keag602 [doi] PST - aheadofprint SO - Rheumatology (Oxford). 2026 Oct 28:keag602. doi: 10.1093/rheumatology/keag602. PMID- 42949085 OWN - NLM STAT- Publisher LR - 20261028 IS - 1945-2403 (Electronic) IS - 0146-4760 (Linking) DP - 2026 Oct 28 TI - Simultaneous determination of etomidate and its derivatives in wastewater with online SPE-HPLC-MS/MS. LID - bkag094 [pii] LID - 10.1093/jat/bkag094 [doi] AB - Etomidate (ETM), a short-acting intravenous sedative, has been increasingly abused, particularly among young people in China and other parts of East Asia, leading to severe health issues and prompting regulatory control. Along with the control of ETM, several new ETM derivatives have emerged as substitutes. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) is a widely used analytical technique for identifying and quantifying abused substances in wastewater, which faces challenges due to low analyte concentrations and matrix interference. This study introduces an online solid-phase extraction (SPE)-HPLC-MS/MS method for the simultaneous determination of ETM, its major metabolite etomidate acid, and eight ETM derivatives in wastewater. The HPLC-MS/MS conditions, including column selection, mobile phase composition, and sample pretreatment conditions, were optimized, respectively. Method validation demonstrated excellent linearity across calibration ranges of 1 to 1000 ng L-1, with limits of detection (LOD) and limits of quantification (LOQ) ranging from 0.2 to 3 ng L-1 and 1 to 10 ng L-1, respectively. The method exhibited satisfactory accuracy with recovery values ranging from 86.6% to 108.6%, and high precision with relative standard deviations between 4.8% and 13.9%. The validated method was successfully applied to analyze 40 authentic wastewater samples, of which four tested positive. The detected compounds included ETM (8.2 and 13.4 ng L-1 in two samples), metomidate (MEM) (6.8 ng L-1), and isopropoxate (IPX) (14.5 ng L-1). These findings make the method suitable for accurate and rapid monitoring of ETM and its derivatives in wastewater, providing a robust analytical tool for future wastewater-based epidemiology investigations. CI - (c) The Author(s) 2026. Published by Oxford University Press. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Wu, Jiahui AU - Wu J AD - Key Laboratory of Drug Monitoring and Control of Zhejiang Province, National Narcotics Laboratory Zhejiang Regional Center, Hangzhou 310053, China. AD - Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, China. FAU - Song, Hong AU - Song H AD - Key Laboratory of Drug Monitoring and Control of Zhejiang Province, National Narcotics Laboratory Zhejiang Regional Center, Hangzhou 310053, China. FAU - Zhao, Peng AU - Zhao P AD - Hangzhou PuYu Technology Development Co., Ltd, Hangzhou 311300, China. FAU - Chen, Xianxin AU - Chen X AD - Key Laboratory of Drug Monitoring and Control of Zhejiang Province, National Narcotics Laboratory Zhejiang Regional Center, Hangzhou 310053, China. FAU - Zhang, Jian AU - Zhang J AD - Key Laboratory of Drug Monitoring and Control of Zhejiang Province, National Narcotics Laboratory Zhejiang Regional Center, Hangzhou 310053, China. AD - Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, China. FAU - Zhu, Bingbin AU - Zhu B AD - Key Laboratory of Drug Monitoring and Control of Zhejiang Province, National Narcotics Laboratory Zhejiang Regional Center, Hangzhou 310053, China. FAU - Fan, Yilei AU - Fan Y AD - Key Laboratory of Drug Prevention and Control Technology of Zhejiang Province, Zhejiang Police College, Hangzhou 310053, China. FAU - Shen, Qing AU - Shen Q AD - Collaborative Innovation Center of Seafood Deep Processing, Zhejiang Province Joint Key Laboratory of Aquatic Products Processing, Institute of Seafood, Zhejiang Gongshang University, Hangzhou 310012, China. FAU - Wang, Haixing AU - Wang H AD - Key Laboratory of Drug Monitoring and Control of Zhejiang Province, National Narcotics Laboratory Zhejiang Regional Center, Hangzhou 310053, China. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - J Anal Toxicol JT - Journal of analytical toxicology JID - 7705085 SB - IM OTO - NOTNLM OT - etomidate OT - etomidate derivatives OT - method validation OT - online SPE-HPLC-MS/MS OT - wastewater-based epidemiology EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 08:12 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:12 [entrez] AID - 9003575 [pii] AID - 10.1093/jat/bkag094 [doi] PST - aheadofprint SO - J Anal Toxicol. 2026 Oct 28:bkag094. doi: 10.1093/jat/bkag094. PMID- 42949082 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1998-3689 (Electronic) IS - 0301-4738 (Linking) VI - 74 IP - 11 DP - 2026 Nov 1 TI - Rapid assessment of diabetes mellitus and diabetic retinopathy in the rural-tribal population of Tapi district of western India. PG - 1676-1683 LID - 10.4103/IJO.IJO_632_26 [doi] AB - BACKGROUND: The present study assessed the prevalence of diabetes mellitus (DM) and diabetic retinopathy (DR) in a rural-tribal population in western India. METHODS: The standard RAAB (Rapid Assessment of Avoidable Blindness) methodology with DR module was used in this survey. A total of 139 clusters of 60 people each aged >/=50 years were selected by cluster random sampling with probability proportionate to size. The participants were classified as having DM if they were known to have DM or if their random blood glucose (RBG) level was >/=200 mg/dL, detected by the test offered at the doorstep. Dilated retinal examination and Scottish DR grading were performed for all diabetics. RESULTS: In total, 7835 (93.9%) out of 8344 eligible participants were examined. The prevalence of DM (381/7835) was 4.9% (95% confidence interval (CI): 4.2-5.6). Of these, 68.5% (261/381) were known diabetics and 31.5% (120/381) were identified by spot RBG test. Fifty-five per cent (n = 144/261) of known diabetics had RBG >/=200 mg/dL. The prevalence of any DR, any maculopathy, and sight-threatening DR was 16.3% (95% CI: 12.5 to 20.1), 9.2% (95% CI: 6.2 to 12.2), and 2.1% (95% CI: 0.7 to 3.5), respectively. CONCLUSION: The prevalence of DM and DR in the rural tribal population was lower than the national average but is emerging. More than half of the diabetics had uncontrolled DM, which needs improved awareness and accessibility to health care services for this underprivileged population. Further studies are needed to establish predictors and causal relationship between prevalence of DM/DR in the rural-tribal population. CI - Copyright (c) 2026 Indian Journal of Ophthalmology. FAU - Gajiwala, Uday AU - Gajiwala U AD - General Ophthalmology, Tejas Eye Hospital, Mandvi, Surat, Gujarat, India. FAU - Shroff, Kavin AU - Shroff K AD - General Ophthalmology, Tejas Eye Hospital, Mandvi, Surat, Gujarat, India. FAU - Pachchigar, Swapnil AU - Pachchigar S AD - General Ophthalmology, Tejas Eye Hospital, Mandvi, Surat, Gujarat, India. FAU - Patel, Dhaval AU - Patel D AD - General Ophthalmology, Tejas Eye Hospital, Mandvi, Surat, Gujarat, India. FAU - Shamanna, B R AU - Shamanna BR AD - School of Medical Science, University of Hyderabad, Hyderabad, Telangana, India. LA - eng PT - Journal Article DEP - 20261028 PL - India TA - Indian J Ophthalmol JT - Indian journal of ophthalmology JID - 0405376 SB - IM MH - Humans MH - India/epidemiology MH - *Diabetic Retinopathy/epidemiology/diagnosis/ethnology MH - *Rural Population/statistics & numerical data MH - Middle Aged MH - Male MH - Prevalence MH - Female MH - Aged MH - *Diabetes Mellitus/epidemiology/diagnosis MH - Cross-Sectional Studies MH - *Mass Screening/methods OTO - NOTNLM OT - Diabetes mellitus OT - diabetic retinopathy OT - rapid assessment of avoidable blindness OT - rural-tribal population EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 08:05 PHST- 2026/03/04 00:00 [received] PHST- 2026/08/04 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:05 [entrez] AID - 02223307-202611000-00016 [pii] AID - 10.4103/IJO.IJO_632_26 [doi] PST - ppublish SO - Indian J Ophthalmol. 2026 Nov 1;74(11):1676-1683. doi: 10.4103/IJO.IJO_632_26. Epub 2026 Oct 28. PMID- 42949072 OWN - NLM STAT- Publisher LR - 20261028 IS - 1437-4331 (Electronic) IS - 1434-6621 (Linking) DP - 2026 Oct 29 TI - Commutability assessment of candidate reference materials for total bilirubin assays in neonates. LID - 10.1515/cclm-2026-1261 [doi] AB - OBJECTIVES: Neonatal total bilirubin (TBIL) measurements are the cornerstone for treatment decisions in neonatal hyperbilirubinemia. However, neonatal TBIL assays suffer from substantial inter-assay differences and standardization is hampered by the lack of commutable secondary reference materials. Therefore, the aim of this study was to assess commutability of candidate reference materials (RMs) to support standardization efforts. METHODS: Three types of materials were investigated as candidate RMs: bilirubin-spiked serum materials prepared by the Dutch EQA provider SKML, residual material from neonatal exchange transfusions, and NIST SRM916b material dissolved in adult serum. Commutability of these materials was assessed using the prediction interval and difference in bias approaches. To this end, all candidate RMs and clinical samples were measured on ten different routine methods and compared to the reference measurement procedure. RESULTS: For three methods, commutability could not be assessed due to too high non-selectivity. The exchange transfusion material demonstrated the highest degree of commutability, the highest TBIL concentration being commutable for all methods. Also, the two SRM-spiked samples with the highest TBIL concentrations showed to be commutable for six out of seven selective methods, except for the Ortho Vitros XT3400 platform. CONCLUSIONS: Exchange transfusion materials provide a robust neonatal serum-based material which can be used as secondary reference material and as EQA material to support and monitor future efforts to improve standardization of neonatal TBIL assays. CI - (c) 2026 the author(s), published by De Gruyter, Berlin/Boston. FAU - van der Hagen, Eline A E AU - van der Hagen EAE AUID- ORCID: 0009-0008-5769-9832 AD - Department of Laboratory Medicine, Radboudumc, Nijmegen, The Netherlands. FAU - Oostendorp, Marlies AU - Oostendorp M AD - Department of Clinical Chemistry and Immunology, Dicoon BV, Location Rijnstate Hospital, Arnhem, The Netherlands. AD - Dutch Foundation for Quality Assessment in Medical Laboratories (SKML), Nijmegen, The Netherlands. FAU - Bioch, Moniek M AU - Bioch MM AUID- ORCID: 0009-0000-7717-252X AD - Department of Laboratory Medicine, Radboudumc, Nijmegen, The Netherlands. AD - Dutch Foundation for Quality Assessment in Medical Laboratories (SKML), Nijmegen, The Netherlands. FAU - Delatour, Vincent AU - Delatour V AD - Scientific and Industrial Metrology Division, Laboratoire national de metrologie et d'essais, Paris, France. FAU - Lo, Stanley F AU - Lo SF AD - Department of Pathology, Children's Wisconsin and Medical College of Wisconsin, Milwaukee, WI, USA. FAU - Grote-Koska, Denis AU - Grote-Koska D AD - Institute of Clinical Chemistry and Central Laboratory, Medizinische Hochschule Hannover, Hannover, Germany. AD - Reference Institute for Bioanalytics, Bonn, Germany. FAU - Staaden, Antje AU - Staaden A AD - Institute of Clinical Chemistry and Central Laboratory, Medizinische Hochschule Hannover, Hannover, Germany. AD - Reference Institute for Bioanalytics, Bonn, Germany. FAU - Frasa, Marieke A M AU - Frasa MAM AD - Laboratory of Clinical Chemistry and Hematology, Jeroen Bosch Hospital, 's-Hertogenbosch, The Netherlands. FAU - Kootstra-Ros, Jenny E AU - Kootstra-Ros JE AUID- ORCID: 0000-0002-1394-513X AD - Dutch Foundation for Quality Assessment in Medical Laboratories (SKML), Nijmegen, The Netherlands. AD - Department of Laboratory Medicine, University Medical Center Groningen, Groningen, The Netherlands. FAU - Westenberg, Lauren E H AU - Westenberg LEH AD - Department of Neonatal and Pediatric Intensive Care, Erasmus MC Sophia Children's Hospital, Division of Neonatology, Rotterdam, The Netherlands. FAU - Vis, Jolande Y AU - Vis JY AD - Department of Clinical Chemistry, Erasmus Medical Center, Rotterdam, The Netherlands. FAU - Schenk, Paul AU - Schenk P AD - Department of Clinical Chemistry and Laboratory Medicine, Leiden University Medical Center, Leiden, The Netherlands. FAU - Tintu, Andrei AU - Tintu A AD - Department of Clinical Chemistry, Erasmus Medical Center, Rotterdam, The Netherlands. FAU - Weykamp, Cas AU - Weykamp C AD - MCA Laboratory, Queen Beatrix Hospital, Winterswijk, The Netherlands. FAU - Hulzebos, Christian V AU - Hulzebos CV AD - Department of Paediatrics, Division of Neonatology, University Medical Center Groningen, Beatrix Children's Hospital, Beatrix Children's Hospital, Groningen, The Netherlands. FAU - van Berkel, Miranda AU - van Berkel M AD - Department of Laboratory Medicine, Radboudumc, Nijmegen, The Netherlands. AD - Dutch Foundation for Quality Assessment in Medical Laboratories (SKML), Nijmegen, The Netherlands. LA - eng PT - Journal Article DEP - 20261029 PL - Germany TA - Clin Chem Lab Med JT - Clinical chemistry and laboratory medicine JID - 9806306 SB - IM OTO - NOTNLM OT - commutability OT - neonatal bilirubin OT - reference measurement procedure OT - standardization OT - total bilirubin assays EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 08:03 PHST- 2026/07/30 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:03 [entrez] AID - cclm-2026-1261 [pii] AID - 10.1515/cclm-2026-1261 [doi] PST - aheadofprint SO - Clin Chem Lab Med. 2026 Oct 29. doi: 10.1515/cclm-2026-1261. PMID- 42949062 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1520-6890 (Electronic) IS - 0009-2665 (Linking) VI - 126 IP - 20 DP - 2026 Oct 28 TI - Regenerated Cellulose for Sustainable Electronic Devices. PG - 11201-11259 LID - 10.1021/acs.chemrev.6c00205 [doi] AB - Regenerated cellulose (RC), derived from natural cellulose through dissolution and regeneration processes, has emerged as a sustainable, versatile, and cost-effective material for advanced electronic devices. With unique hierarchical structures, abundant hydroxy groups, and excellent mechanical and thermal properties, RC allows extensive customization of its characteristics, offering biocompatibility, flexibility, processability, and environmental friendliness. This review systematically summarizes recent advances in RC-based electronics, focusing on its multiscale molecular configuration, dissolution and regeneration mechanisms, and functionalization and engineering strategies. It first provides a detailed molecular-level analysis of RC structures and formation mechanisms. The principles and strategies of cellulose dissolution and regeneration are then discussed, with emphasis on solvent systems and structure-regulating mechanisms essential for processing control. Functionalization strategies are categorized into direct modification and hybridization with functional materials. Notably, the hybridization approach involves the development of conductive, semiconductive, and insulating RC composites, evaluated from the perspectives of material selection, interfacial design, and processing techniques. Subsequently, engineering approaches to enhance mechanical strength, electrical conductivity, and optical transparency are explored. Applications of RC in sensors, actuators, circuits, energy harvesting and storage devices, and electromagnetic shielding devices are highlighted, demonstrating its potential to drive sustainable innovation in electronics. Finally, future directions are proposed to address remaining challenges, paving the way for RC to contribute to eco-friendly, high-performance electronic devices. CI - (c) 2026 American Chemical Society. FAU - Wang, Xiaojuan AU - Wang X AUID- ORCID: 0009-0000-8503-4696 AD - State Key Laboratory of Bio-based Fiber Materials, Tianjin University of Science & Technology, Tianjin300457, China. AD - Joint Key Laboratory of the Ministry of Education, Institute of Applied Physics and Materials Engineering, University of Macau, Taipa, Macau SAR999078, China. FAU - Fan, Mengmeng AU - Fan M AD - State Key Laboratory of Bio-based Fiber Materials, Tianjin University of Science & Technology, Tianjin300457, China. FAU - Qian, Huanyu AU - Qian H AD - State Key Laboratory of Bio-based Fiber Materials, Tianjin University of Science & Technology, Tianjin300457, China. FAU - Pan, Xiaosen AU - Pan X AUID- ORCID: 0000-0002-2760-4598 AD - State Key Laboratory of Bio-based Fiber Materials, Tianjin University of Science & Technology, Tianjin300457, China. FAU - Chen, Lu AU - Chen L AD - Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory, Hubei Provincial Engineering Research Center of Emerging Functional Coating Materials, School of Resource and Environmental Sciences, Wuhan University, Wuhan430079, China. FAU - Jiang, Junzhi AU - Jiang J AD - State Key Laboratory of Bio-based Fiber Materials, Tianjin University of Science & Technology, Tianjin300457, China. FAU - Chen, Chaoji AU - Chen C AUID- ORCID: 0000-0001-9553-554X AD - Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory, Hubei Provincial Engineering Research Center of Emerging Functional Coating Materials, School of Resource and Environmental Sciences, Wuhan University, Wuhan430079, China. FAU - Liu, Hongbin AU - Liu H AD - State Key Laboratory of Bio-based Fiber Materials, Tianjin University of Science & Technology, Tianjin300457, China. FAU - Gao, Meng AU - Gao M AUID- ORCID: 0000-0002-3018-6657 AD - State Key Laboratory of Bio-based Fiber Materials, Tianjin University of Science & Technology, Tianjin300457, China. AD - Joint Key Laboratory of the Ministry of Education, Institute of Applied Physics and Materials Engineering, University of Macau, Taipa, Macau SAR999078, China. LA - eng GR - 2026EHA024/Hubei Provincial International Science and Technology Cooperation Project/ GR - 22678442/National Natural Science Foundation of China/ GR - MYRG-SRG2026-00044-IAPME/University of Macau Multi-Year Research Grant Start-up Research Grant/ GR - NA/Hubei Provincial International Science and Technology Cooperation Project/ GR - NA/National Natural Science Foundation of China/ PT - Journal Article PL - United States TA - Chem Rev JT - Chemical reviews JID - 2985134R SB - IM EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 08:03 PHST- 2026/03/01 00:00 [received] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/08/14 00:00 [revised] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:03 [entrez] AID - 5452759 [pii] AID - 10.1021/acs.chemrev.6c00205 [doi] PST - ppublish SO - Chem Rev. 2026 Oct 28;126(20):11201-11259. doi: 10.1021/acs.chemrev.6c00205. PMID- 42949057 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1520-6890 (Electronic) IS - 0009-2665 (Linking) VI - 126 IP - 20 DP - 2026 Oct 28 TI - Asymmetric Dipolar Cycloadditions toward Chiral Medium-Sized Rings under Palladium and Iridium Catalysis. PG - 11413-11459 LID - 10.1021/acs.chemrev.6c00441 [doi] AB - Chiral medium-sized rings, defined herein as 7- to 11-membered cyclic frameworks, are common in bioactive molecules and occupy an important but still underexplored region of drug-like chemical space. Their asymmetric synthesis remains formidably challenging, as conformational flexibility, transannular interactions, and unfavorable entropic and enthalpic factors collectively complicate ring formation and stereocontrol. In recent years, palladium- and iridium-catalyzed asymmetric dipolar cycloadditions and related cascade processes have emerged as effective strategies for building structurally diverse and stereochemically complex medium-sized rings. As such, this review aims to summarize the advances in this emerging field from the perspectives of metal identity, precursor class, and dipole-generation mode, with emphasis on their effects on chemo-, regio-, diastereo-, and enantioselectivity. We also highlight the distinct yet complementary features of Pd and Ir catalysis, as well as current limitations in generality, selectivity, and substrate scope. By combining mechanistic insight with representative applications, this review aims to define the foundations, boundaries, and future opportunities of this rapidly evolving area. CI - (c) 2026 American Chemical Society. FAU - Li, Yu-Jie AU - Li YJ AD - Engineering Research Center of Photoenergy Utilization for Pollution Control and Carbon Reduction, Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan, Hubei430079, China. FAU - Wang, Xuerui AU - Wang X AD - Engineering Research Center of Photoenergy Utilization for Pollution Control and Carbon Reduction, Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan, Hubei430079, China. FAU - Wang, Qiang AU - Wang Q AD - Engineering Research Center of Photoenergy Utilization for Pollution Control and Carbon Reduction, Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan, Hubei430079, China. FAU - Deng, Wei-Ping AU - Deng WP AUID- ORCID: 0000-0002-4232-1318 AD - Key Laboratory of the Ministry of Education for Advanced Catalysis Materials, College of Chemistry and Materials Science, Zhejiang Normal University, Jinhua, Zhejiang321004, China. FAU - Xiao, Wen-Jing AU - Xiao WJ AUID- ORCID: 0000-0002-9318-6021 AD - Engineering Research Center of Photoenergy Utilization for Pollution Control and Carbon Reduction, Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan, Hubei430079, China. AD - Wuhan Institute of Photochemistry and Technology, Wuhan, Hubei430082, China. FAU - Lu, Liang-Qiu AU - Lu LQ AUID- ORCID: 0000-0003-2177-4729 AD - Engineering Research Center of Photoenergy Utilization for Pollution Control and Carbon Reduction, Ministry of Education, College of Chemistry, Central China Normal University, 152 Luoyu Road, Wuhan, Hubei430079, China. AD - Wuhan Institute of Photochemistry and Technology, Wuhan, Hubei430082, China. AD - School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan453007, China. LA - eng GR - 22203034/National Natural Science Foundation of China/ GR - 22271113/National Natural Science Foundation of China/ GR - 22471089/National Natural Science Foundation of China/ GR - 2023YFA1507203/National Key Research and Development Program of China/ GR - 2024YFA1509702/National Key Research and Development Program of China/ GR - GHY2023KF006/Wuhan Institute of Photochemistry and Technology/ PT - Journal Article PL - United States TA - Chem Rev JT - Chemical reviews JID - 2985134R SB - IM EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 08:03 PHST- 2026/05/08 00:00 [received] PHST- 2026/09/07 00:00 [accepted] PHST- 2026/08/12 00:00 [revised] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:03 [entrez] AID - 5448175 [pii] AID - 10.1021/acs.chemrev.6c00441 [doi] PST - ppublish SO - Chem Rev. 2026 Oct 28;126(20):11413-11459. doi: 10.1021/acs.chemrev.6c00441. PMID- 42949047 OWN - NLM STAT- Publisher LR - 20261028 IS - 2050-7518 (Electronic) IS - 2050-750X (Linking) DP - 2026 Oct 28 TI - Self-interpreting quasi-site-rich electroanalytical interface for autonomous biosensing of hepatotoxic drugs. LID - 10.1039/d6tb01454b [doi] AB - A carbon microfiber is engineered into a self-interpreting electroanalytical interface for on-site monitoring of therapeutic drug-levels. Through a voltammetric self-regulation strategy, the microfiber reconstructs into nanocrystalline domains enriched with naturally occurring quinone/hydroquinone (Q/HyQ) redox moieties, evolving into quasi-site architectures with a high electroactive site density (2.42 x 10(-8) mol cm(-2)), which delivers an exceptional electron-transfer interface with a turnover frequency of 0.134 s(-1). This metal-free interface enables sensitive and selective detection of acetaminophen (APAP) and diclofenac (DCF) drug molecules, even at low abundance (LOD's: 0.89 +/- 0.04 nM, 4.61 +/- 0.32 nM), exhibiting strong selectivity against physiological interferents (<10% deviation) and stable analytical performance in PBS and human serum ( approximately 97% signal retention) with excellent serum recovery ( approximately 98%), demonstrating its potential for on-site monitoring. The engineered interface is experimentally calibrated for practical purposes and digitally integrated with a smartphone-deployable application, HepatoAI, enabling rapid interpretation of electrochemical signals into actionable therapeutic decisions. This work demonstrates that engineered carbon microinterfaces can be seamlessly integrated into intelligent fiber-based devices and electronics for autonomous human healthcare monitoring. FAU - Hefayathullah, Mohamed AU - Hefayathullah M AD - Materials Electrochemistry Laboratory, Department of Chemistry, SRM Institute of Science and Technology, Kattankulathur - 603 203, Chengalpattu, Tamil Nadu, India. FAU - Maduraiveeran, Govindhan AU - Maduraiveeran G AUID- ORCID: 0000-0003-1190-7022 AD - Materials Electrochemistry Laboratory, Department of Chemistry, SRM Institute of Science and Technology, Kattankulathur - 603 203, Chengalpattu, Tamil Nadu, India. AD - Materials Research Center, American University of Sharjah, Sharjah-26666, United Arab Emirates. mgovindhan@aus.edu. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - J Mater Chem B JT - Journal of materials chemistry. B JID - 101598493 SB - IM EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 08:03 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:03 [entrez] AID - 10.1039/d6tb01454b [doi] PST - aheadofprint SO - J Mater Chem B. 2026 Oct 28. doi: 10.1039/d6tb01454b. PMID- 42949043 OWN - NLM STAT- Publisher LR - 20261028 IS - 1478-7083 (Electronic) IS - 0035-8843 (Linking) DP - 2026 Oct 28 TI - Late invasive breast cancer after mastectomy for incidentally detected male DCIS: recurrence or new primary? LID - 10.1308/rcsann.2026.0109 [doi] AB - Ductal carcinoma in situ (DCIS) in men is rare, and its long-term behaviour is not well defined. We report a case of late invasive breast cancer more than two decades after a mastectomy for incidentally detected high-grade DCIS in a 45-year-old man. This case highlights that, although mastectomy for pure DCIS offers excellent local control, invasive occurrence of new primary or local recurrence can still occur. Ongoing awareness and follow-up may therefore be warranted even in male patients treated definitively for DCIS. FAU - Elayyan, R AU - Elayyan R AD - The Rotherham NHS Foundation Trust, UK. FAU - In't Hout, B AU - In't Hout B AD - The Rotherham NHS Foundation Trust, UK. FAU - Caldon, L AU - Caldon L AD - The Rotherham NHS Foundation Trust, UK. FAU - Ranawaka, N AU - Ranawaka N AD - The Rotherham NHS Foundation Trust, UK. FAU - Oweis, R AU - Oweis R AD - The Rotherham NHS Foundation Trust, UK. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Ann R Coll Surg Engl JT - Annals of the Royal College of Surgeons of England JID - 7506860 SB - IM OTO - NOTNLM OT - Ductal carcinoma in situ OT - Gynaecomastia OT - Invasive ductal carcinoma OT - Male breast cancer OT - Mastectomy EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 08:03 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 08:03 [entrez] AID - 10.1308/rcsann.2026.0109 [doi] PST - aheadofprint SO - Ann R Coll Surg Engl. 2026 Oct 28. doi: 10.1308/rcsann.2026.0109. PMID- 42949034 OWN - NLM STAT- Publisher LR - 20261028 IS - 1536-3694 (Electronic) IS - 0163-4356 (Linking) DP - 2026 Oct 28 TI - Quantification of Upadacitinib, Dasatinib, Ruxolitinib, Imatinib, and Cabozantinib in Plasma With Liquid Chromatography-Tandem Mass Spectrometry. LID - 10.1097/FTD.0000000000001535 [doi] AB - BACKGROUND: Tyrosine kinase inhibitors (TKIs) are increasingly used to treat hematological malignancies and solid tumors. As pharmacokinetic variability is substantial among patients, therapeutic drug monitoring may help optimize treatment efficacy and minimize toxicity. However, simultaneous quantification of multiple TKIs with markedly different therapeutic concentration ranges is challenging. A sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous quantification of upadacitinib, dasatinib, ruxolitinib, imatinib, and cabozantinib in human plasma. METHODS: Proteins were precipitated in methanol containing stable isotope-labeled internal standards. Rapid chromatographic separation (5 minutes) was achieved on a BEH C18 column using a gradient of 0.025% ammonium hydroxide in water and methanol. TKIs were detected using a triple-quadrupole mass spectrometry in positive electrospray ionization mode. EMA ICH M10 guidelines were used for method validation. RESULTS: All analytes showed excellent linearity (R2 >/= 0.9992) over calibration ranges of 0.1-10,000 mcg/L depending on the analyte. Lower limits of quantification were 0.1 mcg/L for upadacitinib and dasatinib, 0.5 mcg/L for ruxolitinib, 2.25 mcg/L for cabozantinib, and 10 mcg/L for imatinib. Within-run and between-run precision were 30 000 colonies per hour) enrichment of microbial producer phenotypes using a colony-based fluorescence signal without engineered reporter constructs and should generalize to other pathways where intrinsic or derivatized fluorescence can be measured at the colony level. FAU - Dror, Moriel J AU - Dror MJ AD - Department of Bioengineering, University of California, Los Angeles, CA, USA. yitang@ucla.edu. FAU - Williamson, Cayden AU - Williamson C AD - Department of Bioengineering, University of California, Los Angeles, CA, USA. yitang@ucla.edu. FAU - Tang, Yi AU - Tang Y AUID- ORCID: 0000-0003-1597-0141 AD - Department of Bioengineering, University of California, Los Angeles, CA, USA. yitang@ucla.edu. AD - Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, CA, USA. AD - Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA. FAU - Di Carlo, Dino AU - Di Carlo D AUID- ORCID: 0000-0003-3942-4284 AD - Department of Bioengineering, University of California, Los Angeles, CA, USA. yitang@ucla.edu. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Lab Chip JT - Lab on a chip JID - 101128948 SB - IM EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 07:53 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 07:53 [entrez] AID - 10.1039/d6lc00434b [doi] PST - aheadofprint SO - Lab Chip. 2026 Oct 28. doi: 10.1039/d6lc00434b. PMID- 42949017 OWN - NLM STAT- Publisher LR - 20261028 IS - 1558-9455 (Electronic) IS - 1558-9447 (Print) IS - 1558-9447 (Linking) DP - 2026 Oct 28 TI - Clinical Outcomes of Pedicled Vascularized Bone Grafting for Scaphoid Nonunion. PG - 15589447261496463 LID - 10.1177/15589447261496463 [doi] LID - 15589447261496463 AB - BACKGROUND: Scaphoid nonunion remains a challenging condition, particularly in the presence of compromised vascularity. Pedicled vascularized bone grafts preserve graft blood supply without microsurgical anastomosis and are widely used in this setting. METHODS: A retrospective review was conducted of 30 consecutive patients treated with pedicled vascularized bone grafting for scaphoid nonunion between January 2021 and January 2023. Dorsal 1,2 intercompartmental supraretinacular artery grafts were used for proximal pole nonunion, while volar radial pedicled grafts were used for waist nonunion. Outcomes assessed included Mayo Wrist Score (MWS), Visual Analog Scale (VAS) pain score, grip strength, and wrist range of motion. Union was evaluated clinically and radiographically. RESULTS: All patients demonstrated significant improvement in pain, wrist motion, grip strength, and functional outcome scores. Mean MWS improved from 51.47 +/- 6.40 to 72.13 +/- 5.76 (P < .001), while mean VAS pain scores decreased from 4.40 +/- 0.50 to 2.03 +/- 0.49 (P < .001). No patient demonstrated persistent nonunion or required revision surgery. Functional outcomes were comparable between dorsal and volar graft techniques. CONCLUSION: Pedicled vascularized bone grafting provides reliable union and functional recovery in scaphoid nonunion. Comparable outcomes between techniques support graft selection based on fracture location and vascular considerations. FAU - Pandey, Vijay Kumar AU - Pandey VK AD - Command Hospital (Eastern Command), Kolkata, India. FAU - Dash, Chinmaya AU - Dash C AUID- ORCID: 0009-0001-1028-5025 AD - Command Hospital (Eastern Command), Kolkata, India. FAU - Singh, Hakam AU - Singh H AD - Command Hospital (Eastern Command), Kolkata, India. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Hand (N Y) JT - Hand (New York, N.Y.) JID - 101264149 SB - IM PMC - PMC13716888 OTO - NOTNLM OT - ICSRA pedicled graft OT - Mayo Wrist Score OT - scaphoid nonunion OT - vascularized bone graft OT - volar radial pedicled graft COIS- The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2027/10/28 CRDT- 2026/10/28 07:43 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 07:43 [entrez] PHST- 2027/10/28 00:00 [pmc-release] AID - 10.1177_15589447261496463 [pii] AID - 10.1177/15589447261496463 [doi] PST - aheadofprint SO - Hand (N Y). 2026 Oct 28:15589447261496463. doi: 10.1177/15589447261496463. PMID- 42949016 OWN - NLM STAT- Publisher LR - 20261028 IS - 2046-0236 (Electronic) IS - 0147-8885 (Linking) DP - 2026 Oct 28 TI - Optimizing an ethylenediaminetetraacetic acid decalcification protocol for diagnostic bone biopsies: experience at an Academic Medical Center. PG - 1-9 LID - 10.1080/01478885.2026.2751403 [doi] AB - Decalcification protocols are necessary for histologic assessment of bone biopsies. Strong acid-based decalcification is rapid but detrimental to nucleic acids, while ethylenediaminetetraacetic acid (EDTA) is less harmful but time-consuming. We aimed to standardize a decalcification protocol to optimize turn-around time and tissue preservation. Diagnostic bone core needle biopsies were radiographed for baseline mineralization, fixed in formalin, and transferred to EDTA. Interval radiography was performed until specimens were deemed sufficiently decalcified. Histotechnologists and pathologists evaluated overall histopathologic quality (great, good, poor). Final specimen radiographs were qualitatively assessed for mineralization density (black, gray, white) and distribution (absent, unifocal, multifocal). Logistic regression models were fit to compare the proportion of cores with adequate section quality by mineralization density and distribution, and to infer dose-response relationships, accounting for age, sex, and repeated measurements. Effect size and statistical significance were reported using odds ratios (OR) for non-poor histology and p-values. Fifty-two core needle bone biopsies were collected from 34 patients. Cases with absent mineralization (100%, 6/6) or unifocal gray mineralization (92%, 12/13) typically showed non-poor histology. Cases with multifocal white mineralization often (60%, 3/5) showed poor histology (absent versus multifocal: beta = 18.42, p = 0.048; black versus white mineralization: beta = 21.091, p = 0.043). Cases with multifocal gray mineralization showed variable results, with 68% (19/28) showing acceptable histology. Minimizing decalcification in select cases may decrease turn-around time without compromising diagnostic accuracy. Complete absence of calcium by specimen radiography is unnecessary to achieve high-quality histologic processing and microscopic analysis. FAU - Ha, Jason AU - Ha J AUID- ORCID: 0009-0005-6150-9067 AD - Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA. FAU - Vidis, Laura J AU - Vidis LJ AD - Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA. AD - Department of Pathology, University of Virginia, Charlottesville, VA, USA. FAU - Goyette, Evan AU - Goyette E AD - Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA. AD - Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, Madison, WI, USA. FAU - Schutz, Shannon N AU - Schutz SN AD - Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA. FAU - Baker, Michael L AU - Baker ML AD - Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA. AD - Geisel School of Medicine at Dartmouth, Hanover, NH, USA. FAU - Levy, Joshua J AU - Levy JJ AUID- ORCID: 0000-0001-8050-1291 AD - Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA. AD - Department of Pathology and Laboratory Medicine, Cedars-Sinai Medical Center, Los Angeles, CA, USA. FAU - Linos, Konstantinos AU - Linos K AUID- ORCID: 0000-0001-9462-652X AD - Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA. AD - Geisel School of Medicine at Dartmouth, Hanover, NH, USA. AD - Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, USA. FAU - Kerr, Darcy A AU - Kerr DA AUID- ORCID: 0000-0002-3228-2044 AD - Department of Pathology and Laboratory Medicine, Dartmouth-Hitchcock Medical Center, Lebanon, NH, USA. AD - Geisel School of Medicine at Dartmouth, Hanover, NH, USA. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - J Histotechnol JT - Journal of histotechnology JID - 8003807 SB - IM OTO - NOTNLM OT - Bone biopsies OT - decalcification OT - ethylenediaminetetraacetic acid OT - quality improvement EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 07:33 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 07:33 [entrez] AID - 10.1080/01478885.2026.2751403 [doi] PST - aheadofprint SO - J Histotechnol. 2026 Oct 28:1-9. doi: 10.1080/01478885.2026.2751403. PMID- 42949002 OWN - NLM STAT- Publisher LR - 20261028 IS - 2050-7518 (Electronic) IS - 2050-750X (Linking) DP - 2026 Oct 28 TI - A targeting delivery platform for the mapping of responses to p53-independent intervention in heterogeneous patient cancer cells. LID - 10.1039/d6tb01990k [doi] AB - Developing new cancer therapeutic strategies has become a central focus in precision oncology. However, a major challenge is the lack of robust models to accurately assess target vulnerability in patient-specific heterogeneous cancer cells, which limits the translation of research findings into effective clinical therapies. In this study, we developed an ex vivo blood-based model utilizing a versatile nano-delivery platform to evaluate the responsiveness of MDM2 knockout as a representative therapeutic approach in circulating malignant cells (CMCs) from patients stratified by their p53 status. In our genome editing plasmid delivery system (p@RPA), protamine sulfate was combined with a cell-penetrating peptide to condense the CRISPR-Cas9 plasmid into nano-cores. These nano-cores were then coated with chondroitin sulfate shells functionalized with the AS1411 aptamer, enabling the selective recognition of CD44 and/or nucleolin overexpressing malignant cells. Using the same vector, a molecular beacon delivery system (MBs@RPA) co-loaded with MB(MDM2) for probing MDM2 mRNA, MB(TP53) for probing TP53 mRNA and MB(TP73) for probing TP73 mRNA was constructed for the in situ evaluation of biomarker changes induced by genome editing. p@RPA demonstrates satisfactory efficacy in mediating gene editing for MDM2 knockout in malignant cells. Notably, the knockout of MDM2 induces cell cycle arrest and leads to a marked upregulation of anti-tumor TAp73 irrespective of their p53 status. With efficient gene editing and mRNA probing achieved in patient-derived CMCs, we establish an ex vivo platform capable of in situ therapeutic efficacy assessment at single-cell resolution to explicitly reveal the underlying cellular heterogeneity across individual CMCs. FAU - Qi, Li-Jin AU - Qi LJ AD - Key Laboratory of Biomedical Polymers of the Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, Hubei, 430072, China. chengsixue@whu.edu.cn. FAU - Han, Di AU - Han D AD - Key Laboratory of Biomedical Polymers of the Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, Hubei, 430072, China. chengsixue@whu.edu.cn. FAU - Huang, Qi-Yang AU - Huang QY AD - Key Laboratory of Biomedical Polymers of the Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, Hubei, 430072, China. chengsixue@whu.edu.cn. FAU - Lei, Jin-Ju AU - Lei JJ AD - Cancer Center, Renmin Hospital of Wuhan University, Wuhan, Hubei, 430060, China. leijinju@whu.edu.cn. FAU - Yang, Chao-Jie AU - Yang CJ AD - Key Laboratory of Biomedical Polymers of the Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, Hubei, 430072, China. chengsixue@whu.edu.cn. FAU - Liao, Xin-Ru AU - Liao XR AD - Key Laboratory of Biomedical Polymers of the Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, Hubei, 430072, China. chengsixue@whu.edu.cn. FAU - Gao, Qing-Yu AU - Gao QY AD - Key Laboratory of Biomedical Polymers of the Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, Hubei, 430072, China. chengsixue@whu.edu.cn. FAU - Fu, Fei-Fei AU - Fu FF AD - Key Laboratory of Biomedical Polymers of the Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, Hubei, 430072, China. chengsixue@whu.edu.cn. FAU - Cheng, Si-Xue AU - Cheng SX AUID- ORCID: 0000-0001-9611-4421 AD - Key Laboratory of Biomedical Polymers of the Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, Hubei, 430072, China. chengsixue@whu.edu.cn. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - J Mater Chem B JT - Journal of materials chemistry. B JID - 101598493 SB - IM EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 07:23 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 07:23 [entrez] AID - 10.1039/d6tb01990k [doi] PST - aheadofprint SO - J Mater Chem B. 2026 Oct 28. doi: 10.1039/d6tb01990k. PMID- 42948989 OWN - NLM STAT- Publisher LR - 20261028 IS - 2532-5264 (Electronic) IS - 1122-0643 (Linking) DP - 2026 Oct 27 TI - Clinical significance of secondary purulent mediastinitis from odontogenic and tonsillogenic origins. LID - 10.4081/monaldi.2026.3744 [doi] AB - The aim of this study was to enhance early diagnostic methods and improve the efficacy of surgical intervention in patients with secondary purulent mediastinitis of odontogenic and tonsillogenic origin through the introduction of novel drainage techniques and surgical approaches. The study methodology involved an analysis of clinical cases of acute purulent mediastinitis of odontogenic and tonsillogenic origin, divided into two groups: the main group (40 patients treated with new methods) and the control group (20 patients treated with traditional methods). Surgical treatment in the main group included the drainage of purulent-necrotic foci via external approaches with active drainage systems. For odontogenic forms, a "P"-shaped approach was employed, facilitating effective pus drainage and providing an improved surgical field overview. For tonsillogenic forms, the Kanshin method was utilized to drain the posterior mediastinum. Treatment was further complemented by antibacterial therapy, electrolyte balance correction, and ventilatory support when required. The results demonstrated that the combined use of these surgical methods yielded high treatment efficacy. All patients who underwent timely surgical intervention and received antibacterial therapy exhibited positive outcomes, allowing their discharge from hospital in satisfactory condition. The study's conclusions underscore the critical importance of early diagnosis, the appropriate selection of surgical techniques, and the integration of multidisciplinary treatment approaches. Emphasis is placed on the necessity of active drainage of purulent-inflammatory foci, tailored surgical approaches based on the localization of the pathology, the application of modern diagnostic methods, and close collaboration between maxillofacial and thoracic surgeons to achieve optimal therapeutic outcomes. FAU - Eshiev, Abdyrakman AU - Eshiev A AD - Department of Surgical Dentistry, with a Course in Pediatric Surgical Dentistry, Osh State University, Osh. FAU - Azimbaev, Nurlan AU - Azimbaev N AD - Department of Surgical Dentistry, with a Course in Pediatric Surgical Dentistry, Osh State University, Osh. FAU - Eshieva, Asel AU - Eshieva A AD - Department of Pathology, Basic and Clinical Pharmacology, Osh State University, Osh. FAU - Kurmanbekov, Nursultan AU - Kurmanbekov N AD - Department of Surgical Dentistry, with a Course in Pediatric Surgical Dentistry, Osh State University, Osh. FAU - Eshiev, Daniiar AU - Eshiev D AD - Department of Maxillofacial Surgery, Osh Interstate United Clinical Hospital, Osh. LA - eng PT - Journal Article DEP - 20261027 PL - Italy TA - Monaldi Arch Chest Dis JT - Monaldi archives for chest disease = Archivio Monaldi per le malattie del torace JID - 9307314 SB - IM OTO - NOTNLM OT - Infectious and inflammatory diseases OT - antiseptic therapy OT - drainage OT - purulonecrotic foci OT - surgical intervention EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 06:55 PHST- 2025/09/26 00:00 [received] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 06:55 [entrez] AID - 10.4081/monaldi.2026.3744 [doi] PST - aheadofprint SO - Monaldi Arch Chest Dis. 2026 Oct 27. doi: 10.4081/monaldi.2026.3744. PMID- 42948983 OWN - NLM STAT- Publisher LR - 20261028 IS - 2036-7392 (Print) IS - 2036-7392 (Linking) DP - 2026 Oct 27 TI - Fast response in facial vitiligo monitored by non-invasive imaging during topical ruxolitinib treatment. LID - 10.4081/dr.2026.10964 [doi] AB - Dear Editor, Vitiligo is a chronic autoimmune disorder affecting 0.5-1% of the population, characterized by progressive melanocyte loss and depigmented macules. Non-segmental vitiligo (NSV) is the most common subtype, typically involving the face and acral areas, with relevant psychosocial impact. Conventional therapies frequently provide delayed or incomplete responses. Recently, topical Janus kinase (JAK) inhibitors, such as ruxolitinib 1.5% cream, have shown promise in promoting repigmentation. [...]. FAU - Feresin, Francesca AU - Feresin F AD - Unit of Dermatology, Department of Clinical Internal, Anesthesiological and Cardiovascular Sciences, Sapienza University of Rome. FAU - Sasso, Francesca Paola AU - Sasso FP AD - Unit of Dermatology, Department of Clinical Internal, Anesthesiological and Cardiovascular Sciences, Sapienza University of Rome. FAU - Di Caro, Alberto AU - Di Caro A AD - Unit of Dermatology, Department of Clinical Internal, Anesthesiological and Cardiovascular Sciences, Sapienza University of Rome. FAU - Nistico, Steven Paul AU - Nistico SP AD - Unit of Dermatology, Department of Clinical Internal, Anesthesiological and Cardiovascular Sciences, Sapienza University of Rome. FAU - Pellacani, Giovanni AU - Pellacani G AD - Unit of Dermatology, Department of Clinical Internal, Anesthesiological and Cardiovascular Sciences, Sapienza University of Rome. FAU - Dattola, Annunziata AU - Dattola A AD - Unit of Dermatology, Department of Clinical Internal, Anesthesiological and Cardiovascular Sciences, Sapienza University of Rome. FAU - Greco, Maria Elisabetta AU - Greco ME AD - Unit of Dermatology, Department of Clinical Internal, Anesthesiological and Cardiovascular Sciences, Sapienza University of Rome. LA - eng PT - Journal Article DEP - 20261027 PL - Italy TA - Dermatol Reports JT - Dermatology reports JID - 101566470 OTO - NOTNLM OT - LC-OCT OT - Vitiligo OT - ruxolitinib EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 06:54 PHST- 2026/06/03 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 06:54 [entrez] AID - 10.4081/dr.2026.10964 [doi] PST - aheadofprint SO - Dermatol Reports. 2026 Oct 27. doi: 10.4081/dr.2026.10964. PMID- 42948980 OWN - NLM STAT- Publisher LR - 20261028 IS - 2050-7518 (Electronic) IS - 2050-750X (Linking) DP - 2026 Oct 28 TI - Magnetothermal pneumatic robot with reversible inflation and contraction for separable microneedle delivery. LID - 10.1039/d6tb01931e [doi] AB - Effective transgastric drug delivery is hindered by the gastric mucosal barrier. To address this challenge, this study introduces a remotely controllable magnetothermal pneumatic microneedle robot. The system consists of a phase-change actuator, made from a bilayer Fe(3)O(4)/Ecoflex magnetic film encapsulating a low-boiling-point liquid, and separable gelatin methacryloyl microneedles embedded with engineered internal microbubbles. When exposed to an alternating magnetic field (AMF), the embedded Fe(3)O(4) nanoparticles generate heat, vaporizing the internal liquid and inducing rapid inflation. This process generates a highly tunable output force that linearly correlates with the inflation ratio, enabling precise microneedle penetration into tissue. Notably, this expansion process is reversible, and combined with the low-shear characteristics of the bubble-embedded microneedles, the contraction process of the magnetothermal pneumatic robot upon switching off the AMF directly achieves microneedle detachment. Under the actuation of the magnetothermal pneumatic robot, the optimized bubble-embedded microneedles exhibit sufficient mechanical strength, achieving a penetration efficiency of up to 90% in ex vivo porcine stomachs, along with a favorable post-penetration detachment efficiency of approximately 70%. In summary, this magneto-thermal platform offers a safe, precise, and minimally invasive strategy to overcome the gastric mucosal barrier, demonstrating great potential for local oral drug delivery. FAU - Yang, Yanxia AU - Yang Y AD - China Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China. FAU - Bu, Hongxiu AU - Bu H AD - Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and BioSensing, College of Chemistry and Chemical Engineering, College of Environmental Science and Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha 410082, China. zhuochen@hnu.edu.cn. FAU - Gan, Chao AU - Gan C AD - China Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research, Ministry of Education, College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081, China. FAU - Li, Yingzi AU - Li Y AD - Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and BioSensing, College of Chemistry and Chemical Engineering, College of Environmental Science and Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha 410082, China. zhuochen@hnu.edu.cn. FAU - Wang, Shen AU - Wang S AD - Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and BioSensing, College of Chemistry and Chemical Engineering, College of Environmental Science and Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha 410082, China. zhuochen@hnu.edu.cn. FAU - Zeng, Jiayu AU - Zeng J AD - Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and BioSensing, College of Chemistry and Chemical Engineering, College of Environmental Science and Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha 410082, China. zhuochen@hnu.edu.cn. FAU - Li, Shengkai AU - Li S AUID- ORCID: 0009-0004-2987-549X AD - Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Key Laboratory of Medical Molecular Biology of Guizhou Province, Guizhou Medical University, Guiyang 550025, China. lishengkai@gmc.edu.cn. FAU - Chen, Zhuo AU - Chen Z AUID- ORCID: 0000-0002-9943-2262 AD - Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo and BioSensing, College of Chemistry and Chemical Engineering, College of Environmental Science and Engineering, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha 410082, China. zhuochen@hnu.edu.cn. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - J Mater Chem B JT - Journal of materials chemistry. B JID - 101598493 SB - IM EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 06:54 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 06:54 [entrez] AID - 10.1039/d6tb01931e [doi] PST - aheadofprint SO - J Mater Chem B. 2026 Oct 28. doi: 10.1039/d6tb01931e. PMID- 42948970 OWN - NLM STAT- Publisher LR - 20261028 IS - 2050-7518 (Electronic) IS - 2050-750X (Print) IS - 2050-750X (Linking) DP - 2026 Oct 28 TI - Effects of segment chemistry on mechanical performance and hemocompatibility in polyurethane heart valve scaffolds. LID - 10.1039/d6tb01865c [doi] AB - Polycarbonate-based and polysiloxane-modified thermoplastic polyurethanes (TPUs) are promising candidates for cardiovascular implants because of their tunable mechanical properties and favorable biocompatibility. In this study, we established comparative formulation-property-hemocompatibility criteria for polymeric heart valve scaffolds through a systematic evaluation of five commercial TPU formulations: carbothane AC-4095A (CB95-AC), carbothane AC-4075A (CB75-AC), carbothane PC-3575A (CB75-PC), chronoflex C65D (CF), and quadrasil ARCS 90A (Qsil). This comparative analysis provides practical material-selection criteria based on the balance between elastic recovery, tear resistance, oxidative stability, and hemocompatibility. CF and CB95-AC exhibited the highest stiffness and tensile strength, whereas CB75-AC and CB75-PC demonstrated greater elasticity and lower residual strain during cyclic loading. CB75-AC exhibited the lowest hysteresis and best elastic recovery; however, it also exhibited low tear resistance and slightly elevated hemolysis ( approximately 4%), highlighting the tradeoff between elastic recovery and mechanical robustness. All TPU materials maintained platelet counts and partial thromboplastin time values within ASTM-defined acceptable ranges. FTIR analysis revealed formulation-dependent differences in urethane carbonyl hydrogen bonding and hard-domain association that were associated with differences in mechanical behavior and surface characteristics. DSC revealed formulation-dependent differences in thermal transitions, whereas WAXD showed broadly similar, predominantly amorphous structures with diffuse scattering centered at approximately 20-21 degrees 2theta and no distinct crystalline reflections. Preliminary short-term implantation of a CB95-AC polymeric mitral valve in an ovine model showed no gross thrombotic attachment on the leaflet surfaces after 22 days. Overall, the results demonstrate formulation-dependent tradeoffs among elastic recovery, tear resistance, oxidative stability, and hemocompatibility in TPU-based heart valve scaffolds. These findings provide practical material-selection criteria for future polymeric heart valve scaffold development. FAU - Sadeghi, Farhad AU - Sadeghi F AUID- ORCID: 0000-0002-6664-9369 AD - Department of Biomedical Engineering, University of California, Irvine, USA. arashkh@uci.edu. FAU - Zamani, Yasaman AU - Zamani Y AUID- ORCID: 0000-0002-3687-2471 AD - Department of Biomedical Engineering, University of California, Irvine, USA. arashkh@uci.edu. FAU - Tacklind, Mia AU - Tacklind M AD - Department of Biomedical Engineering, University of California, Irvine, USA. arashkh@uci.edu. FAU - Edwards, Robert AU - Edwards R AD - Department of Pathology & Laboratory Medicine, University of California, Irvine, USA. FAU - Simionescu, Dan AU - Simionescu D AUID- ORCID: 0000-0002-7117-9904 AD - Department of Bioengineering, Clemson University, Clemson, USA. FAU - Kheradvar, Arash AU - Kheradvar A AUID- ORCID: 0000-0003-3864-1359 AD - Department of Biomedical Engineering, University of California, Irvine, USA. arashkh@uci.edu. AD - Department of Radiological Sciences, University of California, Irvine, USA. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - J Mater Chem B JT - Journal of materials chemistry. B JID - 101598493 SB - IM PMC - PMC13717077 MID - NIHMS2214090 COIS- CONFLICT OF INTEREST: The authors do not have any conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/29 CRDT- 2026/10/28 06:43 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 06:43 [entrez] PHST- 2026/10/29 00:00 [pmc-release] AID - 10.1039/d6tb01865c [doi] PST - aheadofprint SO - J Mater Chem B. 2026 Oct 28:10.1039/d6tb01865c. doi: 10.1039/d6tb01865c. PMID- 42948968 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1460-2091 (Electronic) IS - 0305-7453 (Print) IS - 0305-7453 (Linking) VI - 81 IP - 11 DP - 2026 Oct 6 TI - MPT64 secretion as a viability-associated immunoassay readout for rapid phenotypic drug susceptibility classification of Mycobacterium tuberculosis: a proof-of-concept study. LID - 10.1093/jac/dkag358 [doi] LID - dkag358 AB - OBJECTIVES: To evaluate MPT64 secretion as a viability-associated readout for rapid phenotypic drug susceptibility testing (pDST) of Mycobacterium tuberculosis. METHODS: We evaluated MPT64-pDST in 130 M. tuberculosis strains: clinical isolates, WHO quality assessment strains and in vitro-selected resistant mutants (for linezolid only). We assessed the diagnostic performance of MPT64-pDST for determining susceptibility to rifampicin, isoniazid and moxifloxacin. As a secondary objective, we explored its performance for linezolid and bedaquiline. MPT64 was quantified by chemiluminescent enzyme immunoassay, and susceptibility was inferred from Log2 fold change relative to drug-free controls. Reference standards were minimum inhibitory concentration testing for rifampicin, isoniazid, linezolid and bedaquiline and MGIT-based DST for moxifloxacin. Performance was evaluated using receiver operating characteristic analysis, full-dataset Youden-optimal candidate cutoffs and 2000-iteration stratified bootstrap out-of-bag (OOB) internal validation. RESULTS: Using drug-specific full-dataset Youden-optimal candidate cutoffs, MPT64-pDST showed high categorical agreement with reference DST, with no false-resistant classifications for rifampicin, isoniazid or moxifloxacin. Resistance-detection sensitivity was 100% for rifampicin, 98% for isoniazid and 90% for moxifloxacin, and out-of-bag bootstrap estimates were comparable to the full-dataset estimates. Exploratory analyses for linezolid demonstrated separation between susceptible and resistant mutants. The single bedaquiline-resistant isolate was clearly distinguishable from 39 bedaquiline-susceptible isolates. CONCLUSIONS: These findings provide proof of concept that the immunoassay-based MPT64-pDST can enable rapid phenotypic susceptibility classification for rifampicin, isoniazid and moxifloxacin. Further validation in larger isolate collections, including isolates with borderline phenotypes, is warranted. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Murase, Yoshiro AU - Murase Y AUID- ORCID: 0000-0003-0240-8352 AD - Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, 3-1-24 Matsuyama, Kiyose, Tokyo 204-8533, Japan. FAU - Takeuchi, Rikiya AU - Takeuchi R AD - R&D Department, TAUNS Laboratories, Inc., Shimizucho, Shizuoka, Japan. FAU - Ohtaki, Yoshiharu AU - Ohtaki Y AD - R&D Department, TAUNS Laboratories, Inc., Shimizucho, Shizuoka, Japan. FAU - Hosoya, Makiko AU - Hosoya M AD - Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, 3-1-24 Matsuyama, Kiyose, Tokyo 204-8533, Japan. FAU - Aono, Akio AU - Aono A AUID- ORCID: 0000-0003-3478-6892 AD - Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, 3-1-24 Matsuyama, Kiyose, Tokyo 204-8533, Japan. FAU - Chikamatsu, Kinuyo AU - Chikamatsu K AD - Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, 3-1-24 Matsuyama, Kiyose, Tokyo 204-8533, Japan. FAU - Morishige, Yuta AU - Morishige Y AD - Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, 3-1-24 Matsuyama, Kiyose, Tokyo 204-8533, Japan. FAU - Igarashi, Yuriko AU - Igarashi Y AD - Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, 3-1-24 Matsuyama, Kiyose, Tokyo 204-8533, Japan. FAU - Bascuna, Joseph Edwin L AU - Bascuna JEL AD - National Tuberculosis Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, Philippines. FAU - Atienza, Gel Anne Marie V AU - Atienza GAMV AD - National Tuberculosis Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, Philippines. FAU - Sedusta, Angeline Grace A AU - Sedusta AGA AD - National Tuberculosis Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, Philippines. FAU - Sacopon, Catherine Ann P L AU - Sacopon CAPL AD - National Tuberculosis Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, Philippines. FAU - Basilio, Ramon P AU - Basilio RP AD - National Tuberculosis Reference Laboratory, Research Institute for Tropical Medicine, Muntinlupa, Philippines. FAU - Kato-Maeda, Midori AU - Kato-Maeda M AUID- ORCID: 0000-0003-0539-2472 AD - Division of Pulmonary and Critical Care Medicine, Zuckerberg San Francisco General, University of California San Francisco, San Francisco, USA. AD - Center for Tuberculosis, University of California San Francisco, San Francisco, USA. FAU - Mitarai, Satoshi AU - Mitarai S AUID- ORCID: 0000-0002-2288-077X AD - Department of Mycobacterium Reference and Research, Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, 3-1-24 Matsuyama, Kiyose, Tokyo 204-8533, Japan. AD - Department of Basic Mycobacteriosis, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. LA - eng GR - 24K11669/Japan Society for the Promotion of Science KAKENHI/ GR - 23fk0108607h/Japan Agency for Medical Research and Development (AMED)/ GR - 5R01AI176409/NH/NIH HHS/United States GR - TAUNS Laboratories, Inc/ PT - Journal Article PL - England TA - J Antimicrob Chemother JT - The Journal of antimicrobial chemotherapy JID - 7513617 RN - 0 (Antitubercular Agents) RN - 0 (Antigens, Bacterial) RN - 0 (MPT64 protein, Mycobacterium tuberculosis) RN - VJT6J7R4TR (Rifampin) RN - V83O1VOZ8L (Isoniazid) SB - IM MH - *Mycobacterium tuberculosis/drug effects/metabolism MH - Microbial Sensitivity Tests/methods MH - *Antitubercular Agents/pharmacology MH - Humans MH - *Antigens, Bacterial/metabolism/analysis MH - Immunoassay/methods MH - Proof of Concept Study MH - *Microbial Viability/drug effects MH - Rifampin/pharmacology MH - Phenotype MH - Isoniazid/pharmacology PMC - PMC13714544 EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2027/10/28 CRDT- 2026/10/28 06:33 PHST- 2026/06/17 00:00 [received] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 06:33 [entrez] PHST- 2027/10/28 00:00 [pmc-release] AID - 9003512 [pii] AID - dkag358 [pii] AID - 10.1093/jac/dkag358 [doi] PST - ppublish SO - J Antimicrob Chemother. 2026 Oct 6;81(11):dkag358. doi: 10.1093/jac/dkag358. PMID- 42948961 OWN - NLM STAT- In-Process LR - 20261029 IS - 1745-3682 (Electronic) IS - 1745-3674 (Print) IS - 1745-3674 (Linking) VI - 97 DP - 2026 Oct 28 TI - Temporary percutaneous trans-tibiofibular K-wire stabilization of selected unstable ankle fractures. PG - 776-778 LID - 10.2340/17453674.2026.46960 [doi] FAU - Overgaard, Anders F AU - Overgaard AF AD - Department of Orthopaedic Surgery and Traumatology, CORI - Centre for Orthopaedic Research and Innovation, Central and West Zealand Hospital, Denmark. anders.overgaard5@gmail.com. FAU - Bloch, Thomas AU - Bloch T AD - Department of Orthopaedic Surgery and Traumatology, CORI - Centre for Orthopaedic Research and Innovation, Central and West Zealand Hospital, Denmark. FAU - Dreyer, Chris H AU - Dreyer CH AD - Department of Orthopaedic Surgery and Traumatology, CORI - Centre for Orthopaedic Research and Innovation, Central and West Zealand Hospital, Denmark. LA - eng PT - Journal Article DEP - 20261028 PL - Sweden TA - Acta Orthop JT - Acta orthopaedica JID - 101231512 SB - IM PMC - PMC13714678 COIS- All authors declare no conflict of interests. Complete disclosure of interest forms according to ICMJE are available on the article page, doi: 10.2340/17453674.2026.46960 EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/28 CRDT- 2026/10/28 06:33 PHST- 2026/10/02 00:00 [received] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 06:33 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - ActaO-97-46960 [pii] AID - 10.2340/17453674.2026.46960 [doi] PST - epublish SO - Acta Orthop. 2026 Oct 28;97:776-778. doi: 10.2340/17453674.2026.46960. PMID- 42948958 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2173-9110 (Electronic) IS - 1135-5727 (Linking) VI - 100 DP - 2026 Oct 23 TI - [Validity of the use of bioimpedance in the assessment of body composition in children and adolescents: a systematic review]. LID - e202610081 [pii] AB - OBJECTIVE: Bioelectrical impedance analysis (BIA) is a non-invasive technique that estimates different body compartments, including fat mass, fat-free mass and total body water, and may complement traditional anthropometric measures. This review aimed to summarise the available evidence on the validity of BIA for assessing body composition in children and adolescents and to identify factors influencing its performance. METHODS: A systematic review of diagnostic methods was conducted in accordance with the Preferred Reporting Items in Systematic Reviews and Meta-Analyses (PRISMA) recommendations. PubMed, EMBASE and the Cochrane Library were searched in February 2025 for studies published between 2010 and 2024. Validation studies in participants younger than eighteen years comparing BIA with reference methods, such as dual-energy X-ray absorptiometry (DXA), isotope dilution, air-displacement plethysmography or multicomponent models, were included. Two reviewers independently screened records and full texts and extracted relevant information. Risk of bias was assessed using QUADAS-2, and findings were narratively synthesised. RESULTS: Fifty-six studies were included; across fifty-three studies with quantifiable sample sizes, approximately 11,100 participants were evaluated. Reported correlations ranged widely (r=0.03-0.98), being higher when population-specific equations were derived in similar cohorts and lower in obesity, young athletes, infants and clinically complex groups, particularly when generic manufacturer equations were applied. Agreement analyses frequently showed clinically relevant individual discrepancies. In overweight/obesity, BIA tended to underestimate fat mass/%body fat and to overestimate fat-free mass. Reliability was high (ICC: approximately 0.93-0.98). CONCLUSIONS: The available evidence supports BIA as a non-invasive, reproducible and reliable tool, useful for group-level assessment, surveillance and, in particular, longitudinal monitoring of body composition in paediatric populations. It does not replace body mass index or reference methods, and its performance depends critically on the device, the equation applied and the clinical context; therefore, caution is warranted at the extremes of body composition, where population-specific equations are recommended. Nevertheless, given its feasibility and capacity to track change over time, BIA may provide complementary information on body composition and contribute to a more health- and cardiometabolic risk-oriented approach to obesity in children and adolescents when results are appropriately interpreted and communicated. FAU - Martin Saborido, Carlos AU - Martin Saborido C AD - Agencia de Evaluacion de Tecnologias Sanitarias. Instituto de Salud Carlos III. Madrid. Espana. FAU - Garcia Carpintero, Esther Elena AU - Garcia Carpintero EE AD - Agencia de Evaluacion de Tecnologias Sanitarias. Instituto de Salud Carlos III. Madrid. Espana. FAU - Pedrosa Perez, Lucia AU - Pedrosa Perez L AD - Agencia de Evaluacion de Tecnologias Sanitarias. Instituto de Salud Carlos III. Madrid. Espana. FAU - Sanchez Piedra, Carlos Alberto AU - Sanchez Piedra CA AD - Agencia de Evaluacion de Tecnologias Sanitarias. Instituto de Salud Carlos III. Madrid. Espana. FAU - Carmona Rodriguez, Montserrat AU - Carmona Rodriguez M AD - Agencia de Evaluacion de Tecnologias Sanitarias. Instituto de Salud Carlos III. Madrid. Espana. FAU - Montes Bentura, David AU - Montes Bentura D AD - Gabinete de la Ministra. Ministerio de Sanidad. Madrid. Espana. FAU - Cabrera-Leon, Andres AU - Cabrera-Leon A AD - Gabinete de la Ministra. Ministerio de Sanidad. Madrid. Espana. AD - Universidad de Granada. Escuela Andaluza de Salud Publica. Granada. Espana. AD - Instituto de Investigacion Biosanitaria (ibs Granada). CIBER de Epidemiologia y Salud Publica (CIBERESP). Instituto de Salud Carlos III. Madrid. Espana. LA - spa PT - English Abstract PT - Journal Article PT - Review PT - Systematic Review TT - Validez del uso de la bioimpedancia en la evaluacion de la composicion corporal infantil y adolescente: revision sistematica. DEP - 20261023 PL - Spain TA - Rev Esp Salud Publica JT - Revista espanola de salud publica JID - 9600212 SB - IM MH - Humans MH - *Body Composition MH - *Electric Impedance MH - Adolescent MH - Child MH - Reproducibility of Results OTO - NOTNLM OT - Adolescent OT - Bioelectrical impedance OT - Body composition OT - Child OT - Dual-energy X-ray absorptiometry OT - Obesity OT - Spain OT - Systematic review COIS- Disclosure The authors report no conflicts of interest in this work. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 06:33 PHST- 2026/02/27 00:00 [received] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 06:33 [entrez] AID - e202610081 [pii] PST - epublish SO - Rev Esp Salud Publica. 2026 Oct 23;100:e202610081. PMID- 42948957 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2173-9110 (Electronic) IS - 1135-5727 (Linking) VI - 100 DP - 2026 Oct 28 TI - [Therapeutic play in pediatric hospitalization: a comprehensive care strategy beyond pain management]. LID - e202610084 [pii] AB - During pediatric hospitalization, invasive and painful procedures are frequently performed and may cause anxiety and fear, particularly among children who, due to their age, may not fully understand the situation. If pain is not adequately managed, it may be associated with negative consequences, such as sleep disturbances, needle phobia, and reluctance or refusal to seek or receive healthcare. Therefore, alleviating pediatric pain is both a right and a responsibility of healthcare institutions, which should implement effective pain management strategies. Among these strategies, non-pharmacological distraction is an intervention used to reduce pain and distress associated with certain procedures. It may include sensory stimuli adapted to the child's age and developmental level. In this context, play has gained relevance as a therapeutic tool, although its effects should be interpreted considering the type of intervention, the clinical context, and the quality of the available evidence. Play promotes children's emotional, cognitive, and social development and may improve their relationship with healthcare professionals and facilitate cooperation during treatment. Therefore, its incorporation from the time of hospital admission has been proposed, supported by specific institutional policies and professional training programs. Hospitals should also be provided with appropriate resources, and interdisciplinary collaboration should be promoted to ensure its effective implementation as a complementary therapeutic tool. From a public health perspective, its systematic integration should also consider equity in access, differences in resources between healthcare settings, professional training, and outcome evaluation. FAU - Gonzalez-Diaz, Ana AU - Gonzalez-Diaz A AD - Facultad de Ciencias de la Salud. Universidad de Granada. Campus de Ceuta. Ceuta. Espana. FAU - Canals-Garzon, Cristina AU - Canals-Garzon C AD - Facultad de Ciencias de la Salud. Universidad de Granada. Campus de Ceuta. Ceuta. Espana. FAU - Gomez-Salgado, Juan AU - Gomez-Salgado J AD - Departamento de Medicina y Salud Publica. Facultad de Ciencias del Trabajo. Universidad de Huelva. Huelva. Espana. AD - Programa de Posgrado en Seguridad y Salud. Universidad Espiritu Santo. Guayaquil. Ecuador. FAU - Suleiman-Martos, Nora AU - Suleiman-Martos N AD - Facultad de Ciencias de la Salud. Universidad de Granada. Campus de Granada. Granada. Espana. FAU - Gomez-Urquiza, Jose Luis AU - Gomez-Urquiza JL AD - Facultad de Ciencias de la Salud. Universidad de Granada. Campus de Ceuta. Ceuta. Espana. LA - spa PT - English Abstract PT - Journal Article PT - Review TT - El juego terapeutico en la hospitalizacion pediatrica: una estrategia de atencion integral mas alla del manejo del dolor. DEP - 20261028 PL - Spain TA - Rev Esp Salud Publica JT - Revista espanola de salud publica JID - 9600212 SB - IM MH - Humans MH - Child MH - *Pain Management/methods MH - *Child, Hospitalized MH - *Hospitalization MH - *Play Therapy MH - Comprehensive Health Care OTO - NOTNLM OT - Anxiety in children OT - Non-pharmacological distraction OT - Pediatric hospitalization OT - Pediatric pain OT - Spain OT - Technology in children's health OT - Therapeutic play COIS- Disclosure The authors report no conflicts of interest in this work. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 06:33 PHST- 2025/07/02 00:00 [received] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 06:33 [entrez] AID - e202610084 [pii] PST - epublish SO - Rev Esp Salud Publica. 2026 Oct 28;100:e202610084. PMID- 42948951 OWN - NLM STAT- Publisher LR - 20261028 IS - 1520-5126 (Electronic) IS - 0002-7863 (Linking) DP - 2026 Oct 28 TI - Continuity-Engineered Single-Atom Chains Regulate Interfacial Microenvironments for Valorized Alcohol Electrooxidation. LID - 10.1021/jacs.6c12432 [doi] AB - Catalytic selectivity is governed not only by active-site identity but also by the interfacial microenvironments surrounding those sites. Although single-atom chains bridge isolated atoms and extended surfaces, whether their atomic continuity can be exploited as a tunable design parameter for chemical synthesis remains unknown. Here we establish continuity engineering as a strategy for programming catalytic microenvironments through a substrate-anchored synthesis platform that enables precise control over one-dimensional single-atom chain architectures. Using ruthenium chains on alpha-MnO2 as a model system, we construct catalysts with graded continuity and uncover a volcano-type relationship between chain continuity and synthetic performance during benzyl alcohol electrooxidation. The continuity-optimized catalyst achieves >90% Faradaic efficiency for benzaldehyde across a wide potential window in acidic media while sustaining current densities up to 400 mA cm-2. Mechanistic studies suggest that optimal continuity promotes cooperative water activation, generating a hydroxyl-rich interfacial microenvironment that accelerates alcohol dehydrogenation while suppressing overoxidation. The resulting aldehydes can be directly integrated into one-pot bond-forming (C-S/N coupling) transformations. More broadly, atomic continuity emerges as a design variable for programming catalytic microenvironments and controlling selective electrosynthesis. CI - (c) 2026 American Chemical Society. FAU - Pan, Rongrong AU - Pan R AD - Department of Chemistry, Tsinghua University, Beijing100084, P. R. China. FAU - Li, Yapeng AU - Li Y AD - Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing100871, P. R. China. FAU - Shi, Yi AU - Shi Y AD - Department of Applied Chemistry, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Chen, Yanxu AU - Chen Y AD - Department of Applied Chemistry, University of Science and Technology of China, Hefei, Anhui230026, P. R. China. FAU - Li, Zongdai AU - Li Z AD - Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing100871, P. R. China. FAU - Li, Runze AU - Li R AD - Department of Chemistry, Tsinghua University, Beijing100084, P. R. China. FAU - Li, Shiyun AU - Li S AUID- ORCID: 0000-0003-1240-1779 AD - Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing100871, P. R. China. FAU - Shen, Ji AU - Shen J AD - School of Physics and Mechanics, Wuhan University of Technology, Wuhan430070, P. R. China. FAU - Yang, Jiarui AU - Yang J AD - Department of Chemistry, City University of Hong Kong, Kowloon, Hong Kong999077, P. R. China. FAU - Zhuang, Zechao AU - Zhuang Z AD - College of Energy, Xiamen University, Xiamen361000, P. R. China. FAU - Wang, Dingsheng AU - Wang D AUID- ORCID: 0000-0003-0074-7633 AD - Department of Chemistry, Tsinghua University, Beijing100084, P. R. China. FAU - Li, Mufan AU - Li M AUID- ORCID: 0000-0002-4575-4055 AD - Beijing National Laboratory for Molecular Sciences, College of Chemistry and Molecular Engineering, Peking University, Beijing100871, P. R. China. LA - eng GR - 2024M751655/China Postdoctoral Science Foundation/ GR - 2023YFA1506801/National Basic Research Program of China (973 Program)/ GR - 22171157/National Natural Science Foundation of China (NSFC)/ GR - 22205167/National Natural Science Foundation of China (NSFC)/ GR - 22325101/National Natural Science Foundation of China (NSFC)/ GR - 22372004/National Natural Science Foundation of China (NSFC)/ GR - 22388102/National Natural Science Foundation of China (NSFC)/ GR - 22405155/National Natural Science Foundation of China (NSFC)/ GR - Z240027/Beijing Municipal Natural Science Foundation/ PT - Journal Article DEP - 20261028 PL - United States TA - J Am Chem Soc JT - Journal of the American Chemical Society JID - 7503056 SB - IM EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 06:13 PHST- 2026/06/17 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 06:13 [entrez] AID - 5459567 [pii] AID - 10.1021/jacs.6c12432 [doi] PST - aheadofprint SO - J Am Chem Soc. 2026 Oct 28. doi: 10.1021/jacs.6c12432. PMID- 42948947 OWN - NLM STAT- Publisher LR - 20261029 IS - 1747-6356 (Electronic) IS - 1747-6348 (Linking) DP - 2026 Oct 29 TI - Navigating complexity: current evidence and recommendations for management of selected, non-malignant pleural effusions. PG - 1-9 LID - 10.1080/17476348.2026.2755101 [doi] AB - INTRODUCTION: Pleural effusions are common and carry substantial morbidity, mortality, and healthcare costs. Management can be particularly challenging when effusions arising from systemic diseases such as congestive heart failure (CHF) or cirrhosis are refractory to medical management or when the effusion occurs due to disruption of the thoracic lymphatic system, as in chylothorax. AREAS COVERED: This review examines current approaches to the diagnosis and management of congestive heart failure-related effusions, hepatic hydrothorax, and chylothorax. In addition, we seek to identify areas for future investigation across all three conditions, particularly the evidence guiding invasive management such as thoracentesis, indwelling pleural catheters, and pleurodesis. To accomplish this, we conducted a literature search in PubMed between 1 August 2026 and 13 August 2026, using a combination of free-text keywords related to the listed effusions. EXPERT OPINION: If medical/conservative management of complicated pleural effusion fails, there are multiple treatment options for each of the discussed effusions, but no clear-cut superior option as the current literature is limited. Future studies will require multi-center and multi-disciplinary collaboration but will likely reveal that treatment should be based on an individual level, accounting for local expertise and incorporating patients' preferences. FAU - Hagen, Christopher AU - Hagen C AD - Department of Medicine, Dartmouth Hitchcock Medical Center,Lebanon, NH, USA. FAU - Ingemi, Molly AU - Ingemi M AD - Pulmonary and Critical Care Medicine, Dartmouth Hitchcock Medical Center, Lebanon, NH, USA. FAU - Feller-Kopman, David AU - Feller-Kopman D AUID- ORCID: 0000-0002-9008-3617 AD - Pulmonary and Critical Care Medicine, Dartmouth Hitchcock Medical Center, Lebanon, NH, USA. FAU - Howe, John AU - Howe J AD - Pulmonary and Critical Care Medicine, Dartmouth Hitchcock Medical Center, Lebanon, NH, USA. LA - eng PT - Journal Article DEP - 20261029 PL - England TA - Expert Rev Respir Med JT - Expert review of respiratory medicine JID - 101278196 SB - IM OTO - NOTNLM OT - Pleural effusion OT - cardiac effusion OT - chylothorax OT - hepatic hydrothorax OT - refractory effusion EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 06:07 PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 06:07 [entrez] AID - 10.1080/17476348.2026.2755101 [doi] PST - aheadofprint SO - Expert Rev Respir Med. 2026 Oct 29:1-9. doi: 10.1080/17476348.2026.2755101. PMID- 42948943 OWN - NLM STAT- Publisher LR - 20261028 IS - 2191-0200 (Electronic) IS - 0334-1763 (Linking) DP - 2026 Oct 28 TI - Magnetoencephalography in Parkinson's disease: diagnostic potential and barriers to clinical translation. LID - 10.1515/revneuro-2026-0216 [doi] AB - Parkinson's disease (PD) is a heterogeneous neurodegenerative disorder with motor and nonmotor manifestations. Diagnosis remains primarily clinical, and validated markers of early disease and subsequent progression are still needed. Magnetoencephalography (MEG) measures neuronal magnetic fields with millisecond temporal resolution and provides a means of characterizing cortical dysfunction. This narrative review critically synthesizes MEG evidence on periodic and aperiodic spectral activity, functional connectivity, network topology, and individual brain fingerprinting, with emphasis on diagnostic relevance and barriers to clinical translation. Recurring findings include spectral slowing and cognitive-network disruption, whereas resting beta power, burst dynamics, and coupling depend on clinical phenotype, treatment state, and analytical choices. Evidence from electroencephalogram (EEG), functional magnetic resonance imaging (fMRI), and invasive recordings is distinguished from direct MEG findings. Internally validated classification of established PD and healthy controls is promising, but does not establish performance in prodromal disease or clinically uncertain parkinsonism. Optically pumped magnetometers may improve recording flexibility while retaining requirements for environmental interference control. Prospective clinical cohorts, standardized acquisition and analysis, reproducibility studies, independent validation, and comparison with clinical assessment are needed to establish incremental diagnostic and prognostic utility. CI - (c) 2026 the author(s), published by De Gruyter, Berlin/Boston. FAU - Xia, Yunwei AU - Xia Y AD - Department of Geriatric Neurology, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing 210029, China. FAU - Ding, Shixin AU - Ding S AD - Department of Geriatric Neurology, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing 210029, China. AD - Jiangsu Key Laboratory of Neurodegeneration, Nanjing Medical University, Nanjing 211166, China. FAU - Zhang, Li AU - Zhang L AUID- ORCID: 0000-0003-1092-7531 AD - Department of Geriatric Neurology, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing 210029, China. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - Germany TA - Rev Neurosci JT - Reviews in the neurosciences JID - 8711016 SB - IM OTO - NOTNLM OT - Parkinson's disease OT - functional connectivity OT - magnetoencephalography OT - neural oscillation EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 06:07 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 06:07 [entrez] AID - revneuro-2026-0216 [pii] AID - 10.1515/revneuro-2026-0216 [doi] PST - aheadofprint SO - Rev Neurosci. 2026 Oct 28. doi: 10.1515/revneuro-2026-0216. PMID- 42948937 OWN - NLM STAT- Publisher LR - 20261028 IS - 1538-8514 (Electronic) IS - 1535-7163 (Linking) DP - 2026 Oct 28 TI - Preclinical Characterization of ADCT-241, an Exatecan-Based Antibody-Drug Conjugate against PSMA for the Treatment of Metastatic Castration-Resistant Prostate Cancer. PG - OF1-OF13 LID - 10.1158/1535-7163.MCT-25-1241 [doi] AB - Prostate-specific membrane antigen (PSMA) is a type II transmembrane glycoprotein with elevated expression in primary and metastatic prostate cancer compared with normal prostate tissues, with low levels in renal proximal tubules, small intestine, and the brain. PSMA is a clinically validated target and is associated with the progression and aggressiveness of the disease. ADCT-241 is a novel antibody-drug conjugate (ADC) composed of the fully human monoclonal antibody 2A10, directed against PSMA, to which the exatecan-containing PL2202 payload was stochastically conjugated with a drug-to-antibody ratio of 4. 2A10 was selected over J591, as it showed good binding affinity, a better half-life, and superior internalization properties and antitumor activity compared with a J591-based ADC. ADCT-241 showed specific, potent killing of prostate cancer cell lines in vitro and antitumor efficacy in vivo in prostate cancer cell line- and patient-derived xenografts. ADCT-241 showed target-dependent internalization, topoisomerase 1 inhibition, cell-cycle arrest at the G2-M-phase followed by apoptosis-induced cell death, and in vitro bystander activity. In rats and cynomolgus monkeys, ADCT-241 was well tolerated at dose levels /=50% of U.S. National Academy of Medicine life-stage-specific Recommended Dietary Allowances or Adequate Intakes for >/=14 vitamins and minerals, including >/=2 vitamins and >/=2 minerals, without exceeding any applicable Tolerable Upper Intake Level. The review included 157 articles: 103 primary intervention reports and 54 interim, secondary, or post hoc reports. The most consistent findings were improvements in circulating folate and vitamins B6 and B12. Effects on other vitamin biomarkers varied by context and baseline nutritional status, whereas mineral biomarkers showed few consistent improvements, except for iodine, iron and zinc, which were context dependent. Potential clinical benefits emerged in selected prenatal and cognitive contexts, although findings varied across outcomes, populations, and comparators. Findings for cardiovascular disease prevention were largely null. Multivitamins were generally well tolerated across the populations studied, including pregnant women, older adults, and surgical patients. However, formulation-specific adverse effects, such as mild gastrointestinal symptoms with some formulations containing iron or zinc, and potential drug-nutrient interactions, such as vitamin K with warfarin, warrant attention. These findings support a nutritional role for broad-spectrum multivitamins without implying universal disease prevention. Priorities for focused systematic reviews and trials include linking related reports, characterizing baseline nutritional status, assessing clinically meaningful outcomes, and consistently reporting formulations and harms. The review was preregistered on Open Science Framework (https://osf.io/t476d). FAU - Fan, Zhongqi AU - Fan Z AD - Think Healthy Group, LLC, Washington, District of Columbia, USA. FAU - Nelson, John M AU - Nelson JM AD - Think Healthy Group, LLC, Washington, District of Columbia, USA. FAU - Wallace, Taylor C AU - Wallace TC AUID- ORCID: 0000-0002-9403-2745 AD - Think Healthy Group, LLC, Washington, District of Columbia, USA. AD - School of Medicine and Health Sciences, George Washington University, Washington, District of Columbia, USA. AD - Gerald J. and Dorothy R. Friedman School of Nutrition Science and Policy, Tufts University, Boston, MA, USA. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - J Diet Suppl JT - Journal of dietary supplements JID - 101249830 SB - IM OTO - NOTNLM OT - Dietary supplements OT - adults OT - adverse effects OT - efficacy OT - micronutrients OT - multivitamin-mineral supplement OT - multivitamins OT - nutritional status OT - scoping review EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 06:00 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 06:00 [entrez] AID - 10.1080/19390211.2025.2597204 [doi] PST - aheadofprint SO - J Diet Suppl. 2026 Oct 28:1-48. doi: 10.1080/19390211.2025.2597204. PMID- 42948930 OWN - NLM STAT- Publisher LR - 20261028 IS - 1029-2454 (Electronic) IS - 0892-7014 (Linking) DP - 2026 Oct 28 TI - Biofouling communities on idle petroleum transfer hoses and their potential dispersal role at a platform in the Brazilian Campos Basin. PG - 1-10 LID - 10.1080/08927014.2026.2746011 [doi] AB - Idle petroleum transfer hoses provide surfaces for attached marine organisms and may subsequently be relocated. This study describes biofouling and visible surface condition at one platform in the Campos Basin, Brazil, after a reported approximately six-month idle period. The dataset comprises 14 reconstructed section records, with approximate total cover estimated visually by one observer from one or two selected monocular ROV stills per record. Recorded cover ranged from 35% to 93%; the unweighted mean was 71.9% (SD 20.0 percentage points). Mean scores were 89.4%, 44.0% and 71.3% in the waterline, near-surface and intermediate categories, respectively. Image-to-section and depth assignments could not be independently verified, and ecological appearance contributed to section assignment. These contrasts are therefore descriptive. Seven broad visual categories were listed in the records. One record included an orange-yellow colonial morphotype originally interpreted as Tubastraea-like; its identity and invasion status remain unresolved. The corresponding 7.1% is the proportion of records containing that report, not coral cover. Eight records were assigned light external surface damage. The observations illustrate biofouling on idle hoses at one platform, but do not demonstrate transport, establishment after relocation, or the frequency of this potential pathway across the Campos Basin. Traceable image sampling and specimen-based identification are needed to evaluate its biosecurity significance. FAU - Albanit, Leticia AU - Albanit L AD - Instituto de Biociencias, Campus do Litoral Paulista, Universidade Estadual Paulista (UNESP), Sao Vicente, Brazil. FAU - Palanch, Maria Fernanda AU - Palanch MF AD - Programa de Pos-Graduacao em Dinamica dos Oceanos e da Terra, Instituto de Geociencias, Universidade Federal Fluminense (UFF), Niteroi, Brazil. FAU - Ribeiro, Caio Cesar AU - Ribeiro CC AUID- ORCID: 0000-0003-4418-0624 AD - ECHO Research Group (Ecotoxicology and Chemical Oceanography), Centro de Ciencias do Mar do Algarve (CCMAR-CIMAR LA), Universidade do Algarve, Faro, Portugal. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Biofouling JT - Biofouling JID - 9200331 SB - IM OTO - NOTNLM OT - ROV imagery OT - marine biosecurity OT - observational case study OT - petroleum transfer hoses OT - taxonomic uncertainty OT - visual cover estimation EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 06:00 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 06:00 [entrez] AID - 10.1080/08927014.2026.2746011 [doi] PST - aheadofprint SO - Biofouling. 2026 Oct 28:1-10. doi: 10.1080/08927014.2026.2746011. PMID- 42948916 OWN - NLM STAT- Publisher LR - 20261028 IS - 1840-2879 (Electronic) IS - 1840-1848 (Linking) DP - 2026 Oct 27 TI - Variations of the Posterolateral Tubercle of the Talus: Geometric Analysis, Angular Safe Zones, and Implications for Talar Fixation Using a Three-Dimensional Simulation Model. LID - 10.5644/ama2006-124.522 [doi] AB - OBJECTIVE: To investigate the prevalence of posterolateral tubercle variants and characterize the simulated safe zones and screw insertion angles for posterior-to-anterior talar screw fixation using individual 3D models from a Northeastern Thai skeletal collection. MATERIALS AND METHODS: A total of 1,106 dry tali from 553 skeletons (301 males and 252 females), aged 22-106 years, were classified according to posterolateral tubercle type. Safe zones for posterior-to-anterior (PA) screw fixation were determined for the left and right tali in both sexes using 3D simulation models, and the widest possible screw insertion angles were simulated. RESULTS: The posterolateral tubercle was classified into three types: Type 1 (almost absent, 1.36%), Type 2 (normal, 45.48%), and Type 3 (enlarged, 53.16%). In selected individual male talus models, the illustrated safe-zone configurations ranged from 20% to 50% on the left and from 30% to 60% on the right. In selected individual female talus models, the illustrated safe-zone configurations ranged from 20% to 70% on the left and from 20% to 50% on the right. CONCLUSION: The enlarged posterolateral tubercle was the most prevalent variant in the Northeastern Thai skeletal sample. The selected individual models illustrated differences in simulated safe-zone configurations and insertion angles according to side and sex. These findings may provide anatomical reference information for preoperative planning of screw fixation involving the posterolateral region of the talus. FAU - Srinark, Orrawan AU - Srinark O AD - Department of Anatomy and Unit of Human Bone Warehouse for Research, Faculty of Medicine, Khon Kaen University, Thailand. FAU - Samrid, Rarinthorn AU - Samrid R AD - Department of Anatomy and Unit of Human Bone Warehouse for Research, Faculty of Medicine, Khon Kaen University, Thailand. FAU - Chaiyamoon, Arada AU - Chaiyamoon A AD - Department of Anatomy and Unit of Human Bone Warehouse for Research, Faculty of Medicine, Khon Kaen University, Thailand. FAU - Phunchago, Nichapa AU - Phunchago N AD - Department of Anatomy and Unit of Human Bone Warehouse for Research, Faculty of Medicine, Khon Kaen University, Thailand. FAU - Poodendaen, Chanasorn AU - Poodendaen C AD - Department of Anatomy, Faculty of Medical Science, Naresuan University, Thailand. FAU - Duangchit, Suthat AU - Duangchit S AD - Department of Physiology, Faculty of Medical Science, Naresuan University, Thailand. FAU - Iamsaard, Sitthichai AU - Iamsaard S AD - Department of Anatomy and Unit of Human Bone Warehouse for Research, Faculty of Medicine, Khon Kaen University, Thailand. sittia@kku.ac.th. LA - eng PT - Journal Article DEP - 20261027 PL - Bosnia and Herzegovina TA - Acta Med Acad JT - Acta medica academica JID - 101587903 SB - IM OTO - NOTNLM OT - Northeastern Thai Skeletal Collection OT - Posterolateral Tubercle OT - Simulation Model OT - Talar Screw Fixation OT - Talus EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 06:00 PHST- 2026/06/18 00:00 [received] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 06:00 [entrez] AID - ama2006-124.522 [pii] AID - 10.5644/ama2006-124.522 [doi] PST - aheadofprint SO - Acta Med Acad. 2026 Oct 27. doi: 10.5644/ama2006-124.522. PMID- 42948901 OWN - NLM STAT- Publisher LR - 20261028 IS - 1095-8290 (Electronic) IS - 0305-7364 (Linking) DP - 2026 Oct 28 TI - Mechanisms of nitrogen-form preference in shoots and roots of invasive and native Xanthium species. LID - mcag346 [pii] LID - 10.1093/aob/mcag346 [doi] AB - BACKGROUND: Nitrogen (N) form preference influences responses of invasive plants to soil N form changes caused by global change and the evolution of their invasiveness, and the differences in root uptake and shoot assimilation for different forms of N affect N form utilization in plants. However, the relative roles of shoots and roots in N-form preference are still unclear for invasive plants. METHODS: We grew the homo- and heterografted seedlings of the nitrate-preferred invasive plant Xanthium strumarium and the ammonium-preferred native plant X. sibiricum under two levels of nitrate and ammonium. KEY RESULTS: Under high N levels, the grafted seedlings with X. strumarium as scion, regardless of the source of the rootstock always preferred nitrate, whereas those with X. sibiricum as scion always preferred ammonium. Higher expression of nitrate reductase genes in leaves of X. strumarium relative to X. sibiricum was associated with its nitrate preference. Regardless of scion origin, the grafted seedlings with X. strumarium as rootstock always accumulated more total biomass and N than those with X. sibiricum as rootstock under both low and high N levels, which was associated with higher root surface area, volume, and NPF6.3 expression in the invasive relative to the native species. CONCLUSIONS: Our results indicate that N-form preference was determined primarily by shoot identity, whereas roots contributed to the magnitude of the response, providing a novel perspective for understanding the mechanisms underlying exotic plant invasion from the viewpoint of N form utilization. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Annals of Botany Company. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Zhang, Chun-Sha AU - Zhang CS AD - Liaoning Key Laboratory for Biological Invasions and Global Changes, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China. FAU - Feng, Wei-Wei AU - Feng WW AD - College of Ecology and Environment, Southwest Forestry University, Kunming 650233, China. FAU - Jiang, Hui AU - Jiang H AD - Liaoning Key Laboratory for Biological Invasions and Global Changes, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China. FAU - Luo, Jia-Jun AU - Luo JJ AD - Liaoning Key Laboratory for Biological Invasions and Global Changes, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China. FAU - Zhang, Zheng AU - Zhang Z AD - Liaoning Key Laboratory for Biological Invasions and Global Changes, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China. FAU - Liu, Ming-Chao AU - Liu MC AD - Liaoning Key Laboratory for Biological Invasions and Global Changes, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China. FAU - Zhang, Xiao-Jia AU - Zhang XJ AUID- ORCID: 0000-0002-1683-4750 AD - Liaoning Key Laboratory for Biological Invasions and Global Changes, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China. FAU - Feng, Yu-Long AU - Feng YL AD - Liaoning Key Laboratory for Biological Invasions and Global Changes, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Ann Bot JT - Annals of botany JID - 0372347 SB - IM OTO - NOTNLM OT - AMT OT - NPF OT - grafting OT - invasive plants OT - nitrogen assimilation enzyme genes OT - nitrogen-form preference OT - shoot-root interaction EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 05:58 PHST- 2026/05/25 00:00 [received] PHST- 2026/10/21 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:58 [entrez] AID - 9003490 [pii] AID - 10.1093/aob/mcag346 [doi] PST - aheadofprint SO - Ann Bot. 2026 Oct 28:mcag346. doi: 10.1093/aob/mcag346. PMID- 42948897 OWN - NLM STAT- Publisher LR - 20261028 IS - 1537-1719 (Electronic) IS - 0737-4038 (Linking) DP - 2026 Oct 28 TI - scEvoSel maps evolutionary selection signals onto single-cell transcriptomic atlases to reveal selection-relevant cellular contexts. LID - msag271 [pii] LID - 10.1093/molbev/msag271 [doi] AB - Selection is a core driving force of evolution. Although genome-wide scanning can identify numerous genomic regions affected by selection, it remains challenging to further reveal the specific cellular contexts in which these selection signals act. To address this issue, we developed scEvoSel, an analytical framework that integrates population-level selection signals with single-cell RNA sequencing data to identify selection-relevant cell types. We calculated DCMS scores based on the population variation data from wild boars and domestic pigs and mapped them to the single-cell atlas of skeletal muscle from wild boars and Duroc pigs. The results showed that scEvoSel accurately identified fibro-adipogenic progenitors (FAPs) and myocytes, which exhibited significant changes in cell proportions, as well as lymphoid cells, which showed breed-associated transcriptional divergence. Moreover, we also calculated DCMS scores derived from population variation data of red junglefowl and Leghorn chickens and mapped them to the single-cell atlas of the liver of Leghorn chicken. Hepatocytes, endothelial cells, Kupffer cells and B cells were identified by scEvoSel as the primary selection-relevant cell types. Additionally, scEvoSel enables the analysis of key genes and functional pathways affected by selection within the corresponding cellular contexts. Overall, scEvoSel provides a framework for resolving population selection signals at single-cell resolution, expands the research dimensions for functional interpretation of selection signals, and offers a methodological tool for investigating cellular contexts associated with selection-driven evolutionary processes and complex trait formation. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution. FAU - Zhao, Simin AU - Zhao S AD - Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, China. FAU - Hu, Yizhou AU - Hu Y AD - Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, China. FAU - Chang, Guobin AU - Chang G AUID- ORCID: 0000-0001-5712-626X AD - Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, China. FAU - Guo, Qixin AU - Guo Q AUID- ORCID: 0009-0007-2468-1559 AD - Key Laboratory of Animal Genetics and Breeding and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, China. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Mol Biol Evol JT - Molecular biology and evolution JID - 8501455 SB - IM OTO - NOTNLM OT - selection signals OT - selection-relevant cell types OT - single-cell RNA sequencing EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 05:58 PHST- 2026/05/22 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:58 [entrez] AID - 9003500 [pii] AID - 10.1093/molbev/msag271 [doi] PST - aheadofprint SO - Mol Biol Evol. 2026 Oct 28:msag271. doi: 10.1093/molbev/msag271. PMID- 42948891 OWN - NLM STAT- Publisher LR - 20261028 IS - 1744-6848 (Electronic) IS - 1744-683X (Linking) DP - 2026 Oct 28 TI - Function, complexity and thermodynamics in adaptive and intelligent soft-matter systems: an information-theoretical conceptual framework. LID - 10.1039/d6sm00618c [doi] AB - The terms responsive, adaptive and intelligent are widely used in the context of materials science and soft matter, but are qualitative in nature. There is currently no quantitative basis on which systems from different areas of materials science can be compared with one another on a common axis. This paper proposes a conceptual framework intended to address this gap. The approach is to treat any material that transforms a stimulus into an output as an information channel. Three classes of material can be distinguished by how the input stimulus maps onto the output effect: a memoryless map (responsive), a state-conditioned map (adaptive), and a feedback-modified channel (intelligent). Three information-theoretic complexity metrics are defined on this basis: configurational diversity I(1), functional selectivity I(2), and stimulus-response information transfer I(3). Treating the material as the information channel leads to a heuristic relationship between the internal complexity of the material and the information transfer achieved. This specifies an associated optimal internal complexity N* that is set by transmission efficiency, stimulus energy and thermal noise. A two-plane benchmarking scheme is proposed: a dynamic plane pairing the volumetric information rate, I(3)/V, with power density P against the Landauer-Berut floor, and a companion static plane (I(1), I(2)). Eighteen representative systems spanning synthetic soft matter, biology and hard-matter architectures are placed on these coordinates to illustrate how the framework can be employed. Within the uncertainties inherent in this approach the systems appear to separate into broad bands above the Landauer-Berut benchmark. The proposed framework establishes the foundations that may lead to validated design rules through community-contributed conventions and further data sets. FAU - Attard, George S AU - Attard GS AUID- ORCID: 0000-0001-8304-0742 AD - School of Chemistry and Chemical Engineering, University of Southampton, Southampton SO17 1BJ, UK. gza@soton.ac.uk. AD - Division of Physical Chemistry, Lund University, Box 124, SE-221 00 Lund, Sweden. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - Soft Matter JT - Soft matter JID - 101295070 SB - IM EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 05:57 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:57 [entrez] AID - 10.1039/d6sm00618c [doi] PST - aheadofprint SO - Soft Matter. 2026 Oct 28. doi: 10.1039/d6sm00618c. PMID- 42948889 OWN - NLM STAT- Publisher LR - 20261028 IS - 1941-3297 (Electronic) IS - 1941-3289 (Linking) DP - 2026 Oct 28 TI - Diastolic Dysfunction in Congenital Heart Disease: A Pressure-Volume Loop Analysis of Borderline Left Heart. PG - e014323 LID - 10.1161/CIRCHEARTFAILURE.126.014323 [doi] AB - BACKGROUND: Diastolic dysfunction is a major driver of morbidity in congenital heart disease, but its mechanisms are poorly understood. It remains unknown whether left ventricular (LV) diastolic dysfunction in borderline left heart patients is caused by a defect in active relaxation or in passive stiffness. We applied gold-standard pressure-volume (PV) loop analysis to define the mechanism of diastolic dysfunction in patients with borderline LV. METHODS: We analyzed invasive PV loops from children with bLV (n=13) and a pragmatic comparison group of patients with congenitally corrected transposition of the great arteries (ccTGA, n=14). PV loops were generated during transient preload alteration. Active relaxation was assessed by the isovolumetric relaxation time constant (tau) and maximal rate of pressure decline (dP/dt(min)) indexed to peak systolic pressure. Passive stiffness was quantified by the stiffness constant (beta), derived from the multibeat end-diastolic PV relationship. To account for size differences, beta was calculated using indexed ventricular volumes (ibeta), and also converted to a dimensionless stiffness constant (beta(w)=betaxventricular wall volume). RESULTS: Active relaxation measures were similar between groups (tau bLV, 28 [23.5-29.5] versus ccTGA, 24 [21-28] msec, P=0.11; dP/dt(min)/peak systolic pressure bLV: -10.6 [-11.0 to -9.4] versus ccTGA: -9.9 [-10.5 to -8.7] s(-)(1), P=0.17). In contrast, patients with bLV had markedly abnormal passive stiffness. The end-diastolic PV relationship was shifted upward and to the left, and the stiffness constants were higher in the bLV group (ibeta bLV, 0.10 [0.04-0.35] versus ccTGA, 0.03 [0.02-0.04] m(2)/mL, P=0.003; beta(w) bLV, 4.08 [1.6-7.52] versus ccTGA, 1.21 [0.95-1.77]; P=0.002). CONCLUSIONS: The primary mechanism of diastolic dysfunction in bLV is increased passive ventricular stiffness, while no differences were detected in active relaxation. These findings suggest a limited role for lusitropic medications and therapeutic strategies that focus on mitigating ventricular stiffness (such as surgical endocardial fibroelastosis resection) may be more valuable. FAU - Thatte, Nikhil AU - Thatte N AUID- ORCID: 0000-0003-2678-8708 AD - Department of Cardiology (N.T., R.C., J.E., M.F., G.M., R.S.B., S.J.G.), Boston Children's Hospital, MA. AD - Department of Pediatrics (N.T., R.C., J.E., M.F., G.M., R.S.B., S.J.G.), Harvard Medical School, Boston, MA. FAU - Liberman, Martin AU - Liberman M AD - Boston University Chobanian and Avedisian School of Medicine, MA (M.L.). FAU - Hammer, Peter E AU - Hammer PE AUID- ORCID: 0000-0003-4151-2777 AD - Department of Cardiac Surgery (P.E.H., E.F., S.E.), Boston Children's Hospital, MA. FAU - Callahan, Ryan AU - Callahan R AUID- ORCID: 0000-0003-0030-7013 AD - Department of Cardiology (N.T., R.C., J.E., M.F., G.M., R.S.B., S.J.G.), Boston Children's Hospital, MA. AD - Now with Division of Cardiology, Department of Pediatrics, Children's Hospital of Philadelphia, PA (R.C.). AD - Department of Pediatrics (N.T., R.C., J.E., M.F., G.M., R.S.B., S.J.G.), Harvard Medical School, Boston, MA. FAU - Esch, Jesse AU - Esch J AUID- ORCID: 0000-0002-4804-6660 AD - Department of Cardiology (N.T., R.C., J.E., M.F., G.M., R.S.B., S.J.G.), Boston Children's Hospital, MA. AD - Department of Pediatrics (N.T., R.C., J.E., M.F., G.M., R.S.B., S.J.G.), Harvard Medical School, Boston, MA. FAU - Farias, Michael AU - Farias M AUID- ORCID: 0000-0002-4770-8116 AD - Department of Cardiology (N.T., R.C., J.E., M.F., G.M., R.S.B., S.J.G.), Boston Children's Hospital, MA. AD - Department of Pediatrics (N.T., R.C., J.E., M.F., G.M., R.S.B., S.J.G.), Harvard Medical School, Boston, MA. FAU - Marx, Gerald AU - Marx G AD - Department of Cardiology (N.T., R.C., J.E., M.F., G.M., R.S.B., S.J.G.), Boston Children's Hospital, MA. AD - Department of Pediatrics (N.T., R.C., J.E., M.F., G.M., R.S.B., S.J.G.), Harvard Medical School, Boston, MA. FAU - Beroukhim, Rebecca S AU - Beroukhim RS AUID- ORCID: 0000-0003-4053-8932 AD - Department of Cardiology (N.T., R.C., J.E., M.F., G.M., R.S.B., S.J.G.), Boston Children's Hospital, MA. AD - Department of Pediatrics (N.T., R.C., J.E., M.F., G.M., R.S.B., S.J.G.), Harvard Medical School, Boston, MA. FAU - Feins, Eric AU - Feins E AUID- ORCID: 0000-0002-9915-4135 AD - Department of Cardiac Surgery (P.E.H., E.F., S.E.), Boston Children's Hospital, MA. AD - Department of Surgery (E.F., S.E.), Harvard Medical School, Boston, MA. FAU - Emani, Sitaram AU - Emani S AUID- ORCID: 0000-0002-6048-2078 AD - Department of Cardiac Surgery (P.E.H., E.F., S.E.), Boston Children's Hospital, MA. AD - Department of Surgery (E.F., S.E.), Harvard Medical School, Boston, MA. FAU - Ghelani, Sunil J AU - Ghelani SJ AUID- ORCID: 0000-0002-4227-8823 AD - Department of Cardiology (N.T., R.C., J.E., M.F., G.M., R.S.B., S.J.G.), Boston Children's Hospital, MA. AD - Department of Pediatrics (N.T., R.C., J.E., M.F., G.M., R.S.B., S.J.G.), Harvard Medical School, Boston, MA. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Circ Heart Fail JT - Circulation. Heart failure JID - 101479941 SB - IM OTO - NOTNLM OT - diastole OT - heart failure OT - inferior vena cava OT - mitral valve OT - morbidity EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 05:57 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:57 [entrez] AID - 10.1161/CIRCHEARTFAILURE.126.014323 [doi] PST - aheadofprint SO - Circ Heart Fail. 2026 Oct 28:e014323. doi: 10.1161/CIRCHEARTFAILURE.126.014323. PMID- 42948888 OWN - NLM STAT- Publisher LR - 20261028 IS - 1942-0080 (Electronic) IS - 1941-9651 (Linking) DP - 2026 Oct 28 TI - Prediction of All-Cause Mortality, Cardiovascular Mortality, and Angiographic Coronary Disease From LVEF Reserve: Findings From the Multicenter REFINE PET Registry. PG - e020062 LID - 10.1161/CIRCIMAGING.126.020062 [doi] AB - BACKGROUND: Left ventricular ejection fraction reserve (LVEF-R), defined as stress EF minus rest EF, is a marker for high-risk coronary artery disease in patients undergoing positron emission tomography myocardial perfusion imaging, but its prognostic and diagnostic significance in large real-world populations remains incompletely defined. We aimed to analyze the association of LVEF-R with mortality and high-risk obstructive coronary artery disease in a large multicenter cohort. METHODS: We analyzed 34 976 patients from 13 sites who underwent gated rest and vasodilator stress positron emission tomography myocardial perfusion imaging from the Registry of Flow and Perfusion Imaging for Artificial Intelligence with PET. Associations between LVEF-R and all-cause mortality and cardiovascular death were assessed using multivariable Cox regression models. In a subset with invasive coronary angiography (n=6843), the relationship between LVEF-R and angiographic disease was evaluated. RESULTS: A total of 9392 (26.9%) patients had an LVEF-R<0%. Over a median follow-up of 4.1 years, lower LVEF-R demonstrated a graded association with higher mortality. Compared with LVEF-R>5%, adjusted hazard ratios for all-cause mortality were 1.20 for LVEF-R 0% to 5% ([95% CI, 1.13-1.28]; P<0.001), 1.32 for -5% to 0% ([95% CI, 1.23-1.42]; P<0.001), and 1.38 for /= 0.9975. Its joint torque distribution was balanced, with a maximum torque /= 0.9910 and torques were concentrated at the wrist and elbow joints, some values being >/=40 Nm. CONCLUSION: The exoskeleton-type upper limb rehabilitation training mechanism demonstrates superior performance in terms of movement trajectory adaptability and joint protection compared to the end-effector-type mechanism, making it more suitable for passive movement assistance and joint range of motion recovery training. CI - (c) 2026 Haoyu Han et al., published by Wroclaw University of Science and Technology. FAU - Han, Haoyu AU - Han H AD - College of Home and Art Design, Northeast Forestry University, Harbin, Heilongjiang, 150000, China. FAU - Zhu, Qinghui AU - Zhu Q AD - College of Home and Art Design, Northeast Forestry University, Harbin, Heilongjiang, 150000, China. FAU - Feng, Li AU - Feng L AD - College of Home and Art Design, Northeast Forestry University, Harbin, Heilongjiang, 150000, China. LA - eng PT - Journal Article DEP - 20261028 PL - Poland TA - Acta Bioeng Biomech JT - Acta of bioengineering and biomechanics JID - 101194794 SB - IM MH - Humans MH - *Upper Extremity/physiopathology MH - Torque MH - Movement/physiology MH - Biomechanical Phenomena MH - Exoskeleton Device MH - Range of Motion, Articular MH - Computer Simulation MH - Equipment Design MH - Models, Biological OTO - NOTNLM OT - Mechanism analysis OT - Mechanism design OT - Rehabilitation device OT - Rehabilitation training OT - Trajectory fitting EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 CRDT- 2026/10/28 05:57 PHST- 2026/03/10 00:00 [received] PHST- 2026/04/21 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:57 [entrez] AID - abb-220943 [pii] AID - 10.37190/abb/220943 [doi] PST - epublish SO - Acta Bioeng Biomech. 2026 Oct 28;28(1):136-151. doi: 10.37190/abb/220943. eCollection 2026 Jan 1. PMID- 42948885 OWN - NLM STAT- Publisher LR - 20261029 IS - 1934-1563 (Electronic) IS - 1934-1482 (Linking) DP - 2026 Oct 28 TI - The effect of different designs of custom foot orthoses on pain and balance in individuals with plantar fasciitis: A randomized controlled trial with 6-month follow-up. LID - 10.1002/pmrj.70241 [doi] AB - BACKGROUND: Plantar fasciitis (PF) is a common cause of heel pain that significantly affects daily activities. Although custom foot orthoses (CFOs) are widely used to treat this condition, there is limited evidence comparing different orthotic designs and their effects on balance parameters. OBJECTIVE: To investigate the effects of different types of CFOs on pain and static and dynamic balance in individuals with PF. DESIGN: Randomized controlled trial. PATIENTS: 120 participants with PF (age 44.3 +/- 10.1 years; 72.5% female; body mass index 26.5 +/- 4.9 kg/m(2)). INTERVENTIONS: All participants were randomly allocated to receive either CFOs without wedge (control group), CFOs with orthotic wedge type 1 (W1), or CFOs with orthotic wedge type 2 (W2), all of which were prescribed for daily weight-bearing activities. MAIN OUTCOME MEASURES: Pain intensity, dynamic balance from the Y Balance Test, and static balance were assessed at baseline, 3-month, and 6-month follow-up. Static balance parameters were measured using a force platform under four conditions: eyes closed on firm surface (ECFS), eyes closed on soft surface (ECSS), eyes open on firm surface (EOFS), and eyes open on soft surface (EOSS). RESULTS: All three interventions significantly reduced pain and improved dynamic balance over the 6-month period (p < .01). The CFO group showed significantly better static balance in center of mass sway during ECFS (p < .01), ECSS (95% confidence ellipse area; p = .042), and EOSS (p < .05) compared to both the W1 and W2 groups. CONCLUSION: Although all three interventions effectively reduced pain and improved dynamic balance. The CFOs without wedge demonstrated greater improvements in static balance parameters under the more challenging conditions (eyes closed and/or soft surface) compared to wedged designs. These findings suggest that basic CFOs may be sufficient for managing pain in PF; however, if balance is a concern, then the selection of specific orthotic designs may be warranted. CI - (c) 2026 American Academy of Physical Medicine and Rehabilitation. FAU - Thanajiravipat, Thanyachat AU - Thanajiravipat T AUID- ORCID: 0009-0005-9275-8277 AD - Independent Orthotics & Prosthetics Specialist, Bangkok, Thailand. FAU - Richards, Jim AU - Richards J AUID- ORCID: 0000-0002-4004-3115 AD - Allied Health Research Unit, University of Lancashire, Preston, UK. FAU - Hengsomboon, Pichaya AU - Hengsomboon P AUID- ORCID: 0000-0002-3388-3546 AD - Faculty of Physical Therapy, Mahidol University, Nakhon Pathom, Thailand. FAU - Hengsomboon, Ninwisan AU - Hengsomboon N AUID- ORCID: 0000-0002-4896-7793 AD - Faculty of Physical Therapy, Mahidol University, Nakhon Pathom, Thailand. FAU - Bovonsunthonchai, Sunee AU - Bovonsunthonchai S AUID- ORCID: 0000-0003-1044-3174 AD - Faculty of Physical Therapy, Mahidol University, Nakhon Pathom, Thailand. FAU - Sakulsriprasert, Prasert AU - Sakulsriprasert P AUID- ORCID: 0000-0002-2694-2544 AD - Faculty of Physical Therapy, Mahidol University, Nakhon Pathom, Thailand. FAU - Harutaichun, Pavinee AU - Harutaichun P AUID- ORCID: 0000-0003-4820-972X AD - Faculty of Physical Therapy, Mahidol University, Nakhon Pathom, Thailand. LA - eng GR - Mahidol University/ PT - Journal Article DEP - 20261028 PL - United States TA - PM R JT - PM & R : the journal of injury, function, and rehabilitation JID - 101491319 SB - IM EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 05:57 PHST- 2026/08/08 00:00 [revised] PHST- 2026/03/18 00:00 [received] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 05:57 [entrez] AID - 10.1002/pmrj.70241 [doi] PST - aheadofprint SO - PM R. 2026 Oct 28. doi: 10.1002/pmrj.70241. PMID- 42948875 OWN - NLM STAT- Publisher LR - 20261028 IS - 1942-0080 (Electronic) IS - 1941-9651 (Linking) DP - 2026 Oct 28 TI - Multimodality Imaging for Left Atrial Appendage Closure: Practical Guidance Across Preprocedural, Intraprocedural, and Postprocedural Phases. PG - e020263 LID - 10.1161/CIRCIMAGING.126.020263 [doi] AB - Left atrial appendage closure is an increasingly used stroke prevention therapy for patients with atrial fibrillation who are unable to maintain long-term anticoagulation. Imaging quality and consistency are central to procedural safety and long-term outcomes, yet practice varies widely across centers. This review presents a practical, phase-based multimodality imaging framework intended to provide guidance and minimum standards for image acquisition and interpretation across all phases of left atrial appendage closure care, including preprocedural planning and thrombus exclusion, intraprocedural guidance and complication detection, and postprocedural surveillance for device-related thrombus and peridevice leak. Because sizing rules, sealing mechanisms, and leak patterns differ between the 2 contemporary platforms, device-specific considerations for the Watchman and Amulet systems are addressed throughout. Transesophageal echocardiography remains the reference standard for thrombus detection and procedural guidance, while cardiac computed tomography offers high spatial resolution, detailed 3-dimensional characterization, and complementary leak assessment, with optimal modality selection influenced by patient characteristics, operator expertise, and institutional resources. Intracardiac echocardiography and mini transesophageal echocardiographic support anesthesia-sparing workflows. Standardized device-related thrombus and peridevice leak classification and harmonized imaging protocols can enhance reproducibility and facilitate cross-center comparisons. Emerging technologies including computed tomography first workflows, 4-dimensional imaging, AI applications, and computational modeling further advance precision planning and surveillance. FAU - Motairek, Issam AU - Motairek I AUID- ORCID: 0000-0003-3294-8659 AD - Department of Cardiovascular Medicine, Cleveland Clinic, OH (I.M., B.A., M.K., S.H., T.T., H.F., A.K., C.L.J., W.I.S., O.M.W., W.A.J.). RINGGOLD: 2569 FAU - Abadie, Bryan AU - Abadie B AUID- ORCID: 0000-0003-0954-1367 AD - Department of Cardiovascular Medicine, Cleveland Clinic, OH (I.M., B.A., M.K., S.H., T.T., H.F., A.K., C.L.J., W.I.S., O.M.W., W.A.J.). RINGGOLD: 2569 FAU - Cremer, Paul C AU - Cremer PC AUID- ORCID: 0000-0002-0794-3306 AD - Division of Cardiology, Bluhm Cardiovascular Institute, Departments of Medicine and Radiology, Northwestern University Feinberg School of Medicine, Chicago, IL (P.C.C.). RINGGOLD: 12244 FAU - Kanj, Mohamed AU - Kanj M AUID- ORCID: 0009-0002-1419-1808 AD - Department of Cardiovascular Medicine, Cleveland Clinic, OH (I.M., B.A., M.K., S.H., T.T., H.F., A.K., C.L.J., W.I.S., O.M.W., W.A.J.). RINGGOLD: 2569 FAU - Harb, Serge AU - Harb S AUID- ORCID: 0000-0002-7442-4928 AD - Department of Cardiovascular Medicine, Cleveland Clinic, OH (I.M., B.A., M.K., S.H., T.T., H.F., A.K., C.L.J., W.I.S., O.M.W., W.A.J.). RINGGOLD: 2569 FAU - Teixeira, Rogerio AU - Teixeira R AD - Servico de Cardiologia, Unidade Local de Saude de Coimbra, EPE, and Faculdade de Medicina, Universidade de Coimbra, Portugal (R.T.). FAU - Nistri, Stefano AU - Nistri S AUID- ORCID: 0000-0003-4723-6646 AD - Cardiology Service CMSR Veneto Medica, Altavilla Vicentina (VI), Italy (S.N.). FAU - Sitges, Marta AU - Sitges M AUID- ORCID: 0000-0003-1300-4732 AD - Cardiovascular Institute, Hospital Clinic, University of Barcelona, IDIBAPS, Spain (M.S.). AD - CIBER CV, Instituto de Salud Carlos III, Madrid (M.S.). FAU - Taigen, Tyler AU - Taigen T AUID- ORCID: 0000-0002-1258-8118 AD - Department of Cardiovascular Medicine, Cleveland Clinic, OH (I.M., B.A., M.K., S.H., T.T., H.F., A.K., C.L.J., W.I.S., O.M.W., W.A.J.). RINGGOLD: 2569 FAU - Ferrini, Heidi AU - Ferrini H AUID- ORCID: 0009-0001-4855-8068 AD - Department of Cardiovascular Medicine, Cleveland Clinic, OH (I.M., B.A., M.K., S.H., T.T., H.F., A.K., C.L.J., W.I.S., O.M.W., W.A.J.). RINGGOLD: 2569 FAU - Kanta, Amy AU - Kanta A AD - Department of Cardiovascular Medicine, Cleveland Clinic, OH (I.M., B.A., M.K., S.H., T.T., H.F., A.K., C.L.J., W.I.S., O.M.W., W.A.J.). RINGGOLD: 2569 FAU - Cardim, Nuno AU - Cardim N AUID- ORCID: 0000-0002-3812-4872 AD - NOVA Medical School, Faculdade de Ciencias da Universidade Nova de Lisboa, Lisbon, Portugal (N.C.). AD - Cardiology Department, Hospital CUF-Descobertas, Lisbon, Portugal (N.C.). FAU - Jellis, Christine L AU - Jellis CL AUID- ORCID: 0000-0002-8610-0533 AD - Department of Cardiovascular Medicine, Cleveland Clinic, OH (I.M., B.A., M.K., S.H., T.T., H.F., A.K., C.L.J., W.I.S., O.M.W., W.A.J.). RINGGOLD: 2569 FAU - Saliba, Walid I AU - Saliba WI AUID- ORCID: 0000-0003-3324-8611 AD - Department of Cardiovascular Medicine, Cleveland Clinic, OH (I.M., B.A., M.K., S.H., T.T., H.F., A.K., C.L.J., W.I.S., O.M.W., W.A.J.). RINGGOLD: 2569 FAU - Wazni, Oussama M AU - Wazni OM AUID- ORCID: 0000-0001-7115-7716 AD - Department of Cardiovascular Medicine, Cleveland Clinic, OH (I.M., B.A., M.K., S.H., T.T., H.F., A.K., C.L.J., W.I.S., O.M.W., W.A.J.). RINGGOLD: 2569 FAU - Jaber, Wael A AU - Jaber WA AUID- ORCID: 0000-0001-5642-2112 AD - Department of Cardiovascular Medicine, Cleveland Clinic, OH (I.M., B.A., M.K., S.H., T.T., H.F., A.K., C.L.J., W.I.S., O.M.W., W.A.J.). RINGGOLD: 2569 CN - Left Atrial Appendage International Working Group LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Circ Cardiovasc Imaging JT - Circulation. Cardiovascular imaging JID - 101479935 SB - IM OTO - NOTNLM OT - anesthesia OT - atrial fibrillation OT - mortality OT - warfarin OT - workflow EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 05:57 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:57 [entrez] AID - 10.1161/CIRCIMAGING.126.020263 [doi] PST - aheadofprint SO - Circ Cardiovasc Imaging. 2026 Oct 28:e020263. doi: 10.1161/CIRCIMAGING.126.020263. PMID- 42948871 OWN - NLM STAT- Publisher LR - 20261028 IS - 1463-9084 (Electronic) IS - 1463-9076 (Linking) DP - 2026 Oct 28 TI - Using an in situ growing process aided by sonication for synthesizing hybrid BCN@Fe(3)O(4) nanocomposites as highly effective and recyclable photocatalysts for solar-light-driven CO(2) conversion to produce sustainable solar chemicals. LID - 10.1039/d6cp02372j [doi] AB - In this work, we report a simple, reproducible, and template-free in situ growth strategy assisted by ultrasonication for the fabrication of boron-doped graphitic carbon nitride-magnetic iron oxide (BCN@Fe(3)O(4)) hybrid nanocomposites. This method enables the uniform anchoring of monodisperse Fe(3)O(4) nanoparticles with an average size of approximately 20-22 nm onto BCN nanosheets, effectively suppressing particle aggregation and ensuring a stable interfacial architecture. The resulting BCN@Fe(3)O(4) hybrid nanocomposite exhibits enhanced photocatalytic performance, achieving selective CO(2) reduction to formic acid (149 +/- 0.10 microM) along with a high 1,4-NADH regeneration yield of 63.66% +/- 0.07%. These performances are comparable to or exceed those of many recently reported graphitic carbon nitride-based photocatalysts for artificial photosynthesis, demonstrating the advantages of the BCN@Fe(3)O(4) heterostructure in facilitating efficient charge separation and photocatalytic CO(2) conversion. This enhanced activity arises from the combined effects of strong visible-light absorption, efficient charge separation, and increased exposure of active surface sites. FAU - Singh, Pragya AU - Singh P AD - Department of Chemistry and Environmental Science, Madan Mohan Malaviya University of Technology, Gorakhpur, 273010, (U.P.), India. rajeshkr_yadav2003@yahoo.co.in. FAU - Yadav, Rajesh K AU - Yadav RK AUID- ORCID: 0000-0002-5320-5259 AD - Department of Chemistry and Environmental Science, Madan Mohan Malaviya University of Technology, Gorakhpur, 273010, (U.P.), India. rajeshkr_yadav2003@yahoo.co.in. FAU - Bathula, Chinna AU - Bathula C AUID- ORCID: 0000-0001-5460-799X AD - Department of Energy and Material Engineering, Dongguk University, Seoul, 04620, Korea. chinnuchem@gmail.com. FAU - Mishra, Shaifali AU - Mishra S AD - Department of Chemistry and Environmental Science, Madan Mohan Malaviya University of Technology, Gorakhpur, 273010, (U.P.), India. rajeshkr_yadav2003@yahoo.co.in. FAU - Sharma, Kanchan AU - Sharma K AD - Department of Chemistry and Environmental Science, Madan Mohan Malaviya University of Technology, Gorakhpur, 273010, (U.P.), India. rajeshkr_yadav2003@yahoo.co.in. FAU - Jaiswal, Surendra K AU - Jaiswal SK AD - Department of Chemistry and Environmental Science, Madan Mohan Malaviya University of Technology, Gorakhpur, 273010, (U.P.), India. rajeshkr_yadav2003@yahoo.co.in. FAU - Rai, Anurag AU - Rai A AD - Department of Chemistry and Environmental Science, Madan Mohan Malaviya University of Technology, Gorakhpur, 273010, (U.P.), India. rajeshkr_yadav2003@yahoo.co.in. FAU - Baeg, Jin Ook AU - Baeg JO AUID- ORCID: 0000-0003-0074-4758 AD - Korea Research Institute of Chemical Technology, N3, 141 Gajeong-ro, Yuseong-gu, Daejeon 34114, South Korea. jobaeg@krict.re.kr. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Phys Chem Chem Phys JT - Physical chemistry chemical physics : PCCP JID - 100888160 SB - IM EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 05:56 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:56 [entrez] AID - 10.1039/d6cp02372j [doi] PST - aheadofprint SO - Phys Chem Chem Phys. 2026 Oct 28. doi: 10.1039/d6cp02372j. PMID- 42948870 OWN - NLM STAT- Publisher LR - 20261028 IS - 1540-8159 (Electronic) IS - 0147-8389 (Linking) DP - 2026 Oct 28 TI - Outcomes of Subcutaneous Implantable Cardioverter Defibrillators in Patients Being Considered for Heart Transplantation: A Retrospective Cohort Study. LID - 10.1111/pace.70469 [doi] AB - INTRODUCTION: Subcutaneous implantable cardioverter defibrillators (S-ICD) are an alternative to transvenous devices (TV-ICD) in patients not requiring bradycardia pacing, resynchronization (CRT) or anti-tachycardia pacing (ATP). At our center, S-ICDs are often preferred in patients being considered for heart transplantation (HTx) without pacing/CRT/ATP needs, as their removal post-HTx avoids retained transvenous leads which can preclude MRI surveillance for graft rejection. We assessed outcomes of S-ICDs in this population. METHODS: We retrospectively reviewed 201 S-ICD implants at our center; July 2016 - March 2025. RESULTS: We identified 33 patients (22 male; age 46 +/- 13 years) who, at time of S-ICD implant, had been referred for HTx evaluation or were referred at any time thereafter. Etiologies were nonischemic cardiomyopathy (n = 18), hypertrophic cardiomyopathy (n = 5), ischemic cardiomyopathy (n = 4), congenital heart disease (n = 4), and myocarditis (n = 2). Median follow-up was 296 days [IQR = 72-798]. Two patients died, both non-arrhythmic. Four patients received appropriate S-ICD therapies and three had inappropriate shocks due to P- or T-wave oversensing (TWOS) (n = 2), or a rapidly conducted atrial arrhythmia (n = 1). Three patients, all with hypertrophic cardiomyopathy, received transvenous device upgrades: one to CRT-P for disease progression, one to TV-ICD for ATP-terminable ventricular tachycardia and one to TV-ICD due to inappropriate shocks from TWOS. Thirteen patients underwent HTx at median 257 days [IQR = 130-453] post S-ICD; three of whom were bridged with HeartMate 3 LVADs. One LVAD patient needed S-ICD deactivation due to electromagnetic interference. There were no retained device components following HTx and S-ICD extraction. CONCLUSION: In selected pre-HTx patients, S-ICDs offer arrhythmic protection while avoiding retained leads post-HTx. CI - (c) 2026 The Author(s). Pacing and Clinical Electrophysiology published by Wiley Periodicals LLC. FAU - Krishnaswamy, Rohan Joshua AU - Krishnaswamy RJ AUID- ORCID: 0000-0002-0774-2184 AD - Department of Electrophysiology and Pacing, St Vincent's Hospital Sydney, Darlinghurst, New South Wales, Australia. AD - Faculty of Medicine, University of New South Wales, Kensington, New South Wales, Australia. FAU - Cherrett, Callum Alec AU - Cherrett CA AUID- ORCID: 0000-0002-2420-0344 AD - Department of Electrophysiology and Pacing, St Vincent's Hospital Sydney, Darlinghurst, New South Wales, Australia. FAU - Adonopulos, Aaron Argyris AU - Adonopulos AA AUID- ORCID: 0009-0002-0881-9777 AD - Faculty of Medicine, University of New South Wales, Kensington, New South Wales, Australia. AD - Department of Cardiothoracic Surgery, St Vincent's Hospital Sydney, Darlinghurst, New South Wales, Australia. FAU - Macdonald, Peter Simon AU - Macdonald PS AUID- ORCID: 0000-0001-5378-2825 AD - Faculty of Medicine, University of New South Wales, Kensington, New South Wales, Australia. AD - Heart and Lung Transplant Unit, St Vincent's Hospital Sydney, Darlinghurst, New South Wales, Australia. AD - Victor Chang Cardiac Research Institute, Darlinghurst, New South Wales, Australia. FAU - Hayward, Christopher Simon AU - Hayward CS AUID- ORCID: 0000-0003-4036-1890 AD - Faculty of Medicine, University of New South Wales, Kensington, New South Wales, Australia. AD - Heart and Lung Transplant Unit, St Vincent's Hospital Sydney, Darlinghurst, New South Wales, Australia. AD - Victor Chang Cardiac Research Institute, Darlinghurst, New South Wales, Australia. FAU - Muthiah, Kavitha AU - Muthiah K AD - Faculty of Medicine, University of New South Wales, Kensington, New South Wales, Australia. AD - Heart and Lung Transplant Unit, St Vincent's Hospital Sydney, Darlinghurst, New South Wales, Australia. AD - Victor Chang Cardiac Research Institute, Darlinghurst, New South Wales, Australia. FAU - Watson, Alasdair John AU - Watson AJ AD - Department of Cardiothoracic Surgery, St Vincent's Hospital Sydney, Darlinghurst, New South Wales, Australia. AD - Heart and Lung Transplant Unit, St Vincent's Hospital Sydney, Darlinghurst, New South Wales, Australia. FAU - Subbiah, Rajesh Natarajan AU - Subbiah RN AD - Department of Electrophysiology and Pacing, St Vincent's Hospital Sydney, Darlinghurst, New South Wales, Australia. AD - Faculty of Medicine, University of New South Wales, Kensington, New South Wales, Australia. AD - Victor Chang Cardiac Research Institute, Darlinghurst, New South Wales, Australia. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Pacing Clin Electrophysiol JT - Pacing and clinical electrophysiology : PACE JID - 7803944 SB - IM OTO - NOTNLM OT - heart transplantation OT - hypertrophic cardiomyopathy OT - inappropriate therapy OT - left ventricular assist device OT - subcutaneous implantable cardioverter defibrillator OT - ventricular arrhythmia EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 CRDT- 2026/10/28 05:56 PHST- 2026/09/21 00:00 [revised] PHST- 2026/08/29 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:56 [entrez] AID - 10.1111/pace.70469 [doi] PST - aheadofprint SO - Pacing Clin Electrophysiol. 2026 Oct 28. doi: 10.1111/pace.70469. PMID- 42948848 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2169-7574 (Print) IS - 2169-7574 (Electronic) IS - 2169-7574 (Linking) VI - 14 IP - 10 DP - 2026 Oct TI - Recurrent Cellulitis in Lymphedema: Integrating Antibiotic Prophylaxis, Conservative Therapy, Local Disease Control, and Lymphaticovenular Anastomosis. PG - e8209 LID - 10.1097/GOX.0000000000008209 [doi] LID - e8209 AB - BACKGROUND: Recurrent cellulitis is a disabling complication of lymphedema, causing repeated antibiotic exposure, hospitalization, anxiety, activity restriction, and worsening edema. Although compression, skin care, early treatment, and antibiotic prophylaxis are established, recurrence may persist when lymphatic dysfunction, lymphorrhea, occult entry portals, or local lymphatic lesions remain unaddressed. This narrative review reframes recurrent cellulitis as chronic lymphatic vulnerability and proposes an integrated prevention framework. METHODS: English-language literature from April 2000 to March 2026 was reviewed using PubMed/MEDLINE, reference lists, consensus documents, and clinical guidelines. Evidence was synthesized narratively across pathophysiology, conservative care, antibiotic prophylaxis, local disease control, lymphatic imaging, lymphaticovenular anastomosis (LVA), and patient education. No quantitative pooling was performed. RESULTS: Compression therapy reduced recurrent cellulitis from 40% to 15% in chronic leg edema, supporting edema control as infection prevention. Antibiotic prophylaxis can reduce recurrence during active treatment but does not correct lymphatic stasis, skin-barrier failure, lymphorrhea, or persistent portals of entry. LVA reduced cellulitis from 1.46 to 0.18 episodes per year in one clinical study, and randomized evidence supports LVA combined with conservative therapy. Local excision combined with genital or lower-limb LVA reduced cellulitis from 2.8 to 0.31 episodes per year in patients with genital lymphatic vesicles. CONCLUSIONS: Durable prevention requires coordinated use of conservative care, antibiotic stabilization, local disease control, lymphatic imaging, selective LVA, and patient education. This multidisciplinary framework may help reduce recurrent febrile cellulitis while supporting safer antibiotic discontinuation, activity, confidence, and quality of life. CI - Copyright (c) 2026 The Authors. Published by Wolters Kluwer Health, LLC. on behalf of The American Society of Plastic Surgeons. FAU - Mihara, Makoto AU - Mihara M AD - From the Lymphedema Clinic Tokyo, Tokyo, Japan. FAU - Hara, Hisako AU - Hara H AD - From the Lymphedema Clinic Tokyo, Tokyo, Japan. AD - Department of Lymphatic and Reconstructive Surgery, JR Tokyo General Hospital, Tokyo, Japan. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Plast Reconstr Surg Glob Open JT - Plastic and reconstructive surgery. Global open JID - 101622231 PMC - PMC13713069 COIS- The authors have no financial interest to declare in relation to the content of this article. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/27 CRDT- 2026/10/28 05:54 PHST- 2026/04/24 00:00 [received] PHST- 2026/08/09 00:00 [revised] PHST- 2026/08/26 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:54 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - GOX-D-26-00505 [pii] AID - 10.1097/GOX.0000000000008209 [doi] PST - epublish SO - Plast Reconstr Surg Glob Open. 2026 Oct 27;14(10):e8209. doi: 10.1097/GOX.0000000000008209. eCollection 2026 Oct. PMID- 42948843 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2005-7806 (Print) IS - 2005-7814 (Electronic) IS - 2005-7806 (Linking) VI - 18 IP - 5 DP - 2026 Oct TI - Evaluation of shear bond strength of 3D-printed flexible denture materials and reline materials. PG - 410-419 LID - 10.4047/jap.2026.18.5.410 [doi] AB - PURPOSE: This study aimed to compare the shear bond strengths (SBS) of two three-dimensionally (3D) printed flexible denture resins and a conventional injection-molded resin bonded with two hard chairside reline materials. MATERIALS AND METHODS: Three flexible denture materials (two 3D-printed resins, including Bright 3D Flexible Denture [Bright] and Tera Harz Flexible Denture [TFDH], and one conventional resin [Con]) were bonded with Ufi Gel Hard (U) and Tokuyama Rebase II (R). In total, 120 specimens were fabricated, relined, and subjected to SBS testing before and after thermocycling. Failure modes were classified as adhesive or mixed. SBS values were analyzed using two- and one-way analyses of variance, t-tests, and Mann-Whitney U tests (alpha = 0.05). RESULTS: Groups bonded with U had significantly higher SBS than those bonded with R across all combinations of the 3D-printed flexible denture resins. TFDH bonded with U exhibited the highest SBS both before (4.78 +/- 1.72 MPa) and after thermocycling (4.05 +/- 0.96 MPa), whereas 80% of TFDH bonded with R specimens and all Bright bonded with R specimens failed after thermocycling. Con bonded with R exhibited the lowest SBS after thermocycling (0.75 +/- 0.39 MPa). Failure mode analysis confirmed the predominance of adhesive failure in the groups bonded with R and mixed failures in the groups bonded with U. CONCLUSION: With appropriate selection of reline materials, 3D-printed flexible denture resin showed significantly higher SBS than that of the conventional flexible denture resin. Appropriate material pairing is critical for achieving durable relining in clinical applications. CI - (c) 2026 The Korean Academy of Prosthodontics. FAU - Liu, Yunshu AU - Liu Y AUID- ORCID: 0009-0000-8154-1666 AD - Department of Prosthodontics, College of Dentistry, Yonsei University, Seoul, Republic of Korea. FAU - Park, Yeseul AU - Park Y AUID- ORCID: 0009-0003-6630-4058 AD - Department of Prosthodontics, Oral and Science Research Center, College of Dentistry, Yonsei University, Seoul, Republic of Korea. FAU - Kim, Jimin AU - Kim J AUID- ORCID: 0009-0004-7755-0972 AD - Department of Prosthodontics, Oral and Science Research Center, College of Dentistry, Yonsei University, Seoul, Republic of Korea. FAU - Kim, Jee-Hwan AU - Kim JH AUID- ORCID: 0000-0002-0872-4906 AD - Department of Prosthodontics, College of Dentistry, Yonsei University, Seoul, Republic of Korea. LA - eng PT - Journal Article DEP - 20261013 PL - Korea (South) TA - J Adv Prosthodont JT - The journal of advanced prosthodontics JID - 101521064 PMC - PMC13713210 OTO - NOTNLM OT - Aging OT - Dental bonding OT - Denture rebasing OT - Removable partial denture OT - Three-dimensional printing EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/01 CRDT- 2026/10/28 05:53 PHST- 2026/01/05 00:00 [received] PHST- 2026/02/26 00:00 [revised] PHST- 2026/03/20 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:53 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - 10.4047/jap.2026.18.5.410 [doi] PST - ppublish SO - J Adv Prosthodont. 2026 Oct;18(5):410-419. doi: 10.4047/jap.2026.18.5.410. Epub 2026 Oct 13. PMID- 42948839 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2169-7574 (Print) IS - 2169-7574 (Electronic) IS - 2169-7574 (Linking) VI - 14 IP - 10 DP - 2026 Oct TI - Soft-tissue Coverage for Complex Knee Defects: The Role of Microsurgery in Optimizing Functional Limb Salvage. PG - e8214 LID - 10.1097/GOX.0000000000008214 [doi] LID - e8214 AB - BACKGROUND: Soft-tissue defects of the knee pose unique reconstructive challenges, and inadequate coverage can have devastating sequelae, including amputation. The purpose of this study is to evaluate the indications and role of microsurgical free flaps, in comparison with pedicled gastrocnemius flaps, for soft-tissue reconstruction of the knee. METHODS: Patients undergoing soft-tissue reconstruction of knee defects from 2018 to 2024 at a tertiary care center were analyzed. Patient characteristics and outcomes were compared for reconstructions using free flaps versus gastrocnemius flaps. The primary outcome was limb salvage with ambulation. Secondary outcomes included patient-reported outcomes and complications. RESULTS: A total of 43 patients comprised the study population: 25 with pedicled gastrocnemius flap and 18 with free flap reconstructions. Limb salvage was achieved in 88.9% of patients with free flaps and 84.0% of patients with pedicled flaps (P = 0.67). Functional outcomes were similar between free and pedicled flaps (mean Lower Extremity Functional Scale scores of 51.2 +/- 13.6 versus 47.5 +/- 12.8, P = 0.40). Defect size was larger in the microsurgical versus pedicled cohort (88 +/- 35 versus 65 +/- 20 cm(2), P = 0.019). Reoperations/secondary procedures were high in both cohorts: 55.6% of patients with free flaps versus 60.0% of patients with pedicled flaps (P = 0.62). CONCLUSIONS: Although pedicled flaps remain a mainstay of treatment algorithms, microsurgical reconstruction offers a safe and versatile option for achieving functional limb salvage in select large, complex knee defects. We advocate for a patient-centered and defect-specific approach to soft-tissue reconstruction of the knee. CI - Copyright (c) 2026 The Authors. Published by Wolters Kluwer Health, LLC. on behalf of The American Society of Plastic Surgeons. FAU - Galvano, Elena AU - Galvano E AD - From the Department of Plastic Surgery, MedStar Health, MedStar Health Washington Hospital Center, Washington, DC. FAU - Kleiber, Grant M AU - Kleiber GM AD - From the Department of Plastic Surgery, MedStar Health, MedStar Health Washington Hospital Center, Washington, DC. FAU - Parikh, Rajiv P AU - Parikh RP AD - From the Department of Plastic Surgery, MedStar Health, MedStar Health Washington Hospital Center, Washington, DC. AD - Section of Plastic Surgery, University of Michigan, Ann Arbor, MI. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Plast Reconstr Surg Glob Open JT - Plastic and reconstructive surgery. Global open JID - 101622231 PMC - PMC13713067 COIS- The authors have no financial interest to declare in relation to the content of this article. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/27 CRDT- 2026/10/28 05:53 PHST- 2026/04/26 00:00 [received] PHST- 2026/08/02 00:00 [revised] PHST- 2026/08/18 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:53 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - GOX-D-26-00514 [pii] AID - 10.1097/GOX.0000000000008214 [doi] PST - epublish SO - Plast Reconstr Surg Glob Open. 2026 Oct 27;14(10):e8214. doi: 10.1097/GOX.0000000000008214. eCollection 2026 Oct. PMID- 42948837 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2168-8184 (Print) IS - 2168-8184 (Electronic) IS - 2168-8184 (Linking) VI - 18 IP - 9 DP - 2026 Sep TI - Clinical Outcomes of the H-Shaped Incision in the Surgical Management of Naso-Orbito-Ethmoidal Fractures: A Prospective Clinical Study. PG - e117015 LID - 10.7759/cureus.117015 [doi] LID - e117015 AB - Background Naso-orbito-ethmoidal fractures are complex central midfacial injuries that require adequate exposure for accurate reduction and stable fixation. This study evaluated the clinical outcomes of an H-shaped incision approach for naso-orbito-ethmoidal fractures. Materials and methods This prospective single-arm study included 20 adults with clinically and radiographically confirmed naso-orbito-ethmoidal fractures requiring open reduction and internal fixation. The central fracture region was exposed through an H-shaped incision with additional infraorbital or intraoral vestibular access when required. Outcomes included operative time, surgical exposure, fracture reduction, pain, facial edema, wound healing, scar quality, neurosensory status, and complications. Patients were followed for six months. Results The mean operative time was 85.15 +/- 7.05 minutes. Surgical exposure was excellent in 12 (60%) patients and good in 8 (40%) patients. Fracture reduction was excellent in 13 (65%) patients and good in 7 (35%) patients. Mean pain scores decreased from 5.25 +/- 0.85 at one week to 1.45 +/- 0.51 at one month (p<0.001). Mean Patient and Observer Scar Assessment Scale scores decreased from 21.95 +/- 2.35 at one month to 10.15 +/- 1.50 at six months (p<0.001). Three (15%) patients developed transient paresthesia. No persistent sensory deficit or clinically apparent medial canthal tendon or nasolacrimal duct injury was recorded. Conclusions The H-shaped incision approach provided adequate surgical exposure and satisfactory fracture reduction with favorable postoperative recovery in selected patients. Larger comparative studies are required to define its relative advantages over established approaches. CI - Copyright (c) 2026, Edsor et al. FAU - Edsor, Effie AU - Edsor E AD - Department of Oral and Maxillofacial Surgery, Bharath Institute of Higher Education and Research, Chennai, IND. AD - Department of Oral and Maxillofacial Surgery, RVS Dental College and Hospital, Coimbatore, IND. FAU - K, Prabhusankar AU - K P AD - Department of Oral and Maxillofacial Surgery, RVS Dental College and Hospital, Coimbatore, IND. FAU - M, Srinisha AU - M S AD - Department of Oral and Maxillofacial Surgery, RVS Dental College and Hospital, Coimbatore, IND. FAU - Ps, Priyadharsana AU - Ps P AD - Department of Oral and Maxillofacial Surgery, RVS Dental College and Hospital, Coimbatore, IND. FAU - K, Vignesh AU - K V AD - Department of Oral and Maxillofacial Surgery, RVS Dental College and Hospital, Coimbatore, IND. FAU - S, Vigneshwar AU - S V AD - Department of Oral and Maxillofacial Surgery, RVS Dental College and Hospital, Coimbatore, IND. LA - eng PT - Journal Article DEP - 20260927 PL - United States TA - Cureus JT - Cureus JID - 101596737 PMC - PMC13712642 OTO - NOTNLM OT - internal fixation OT - maxillofacial trauma OT - naso-orbito-ethmoidal fracture OT - open reduction OT - scar assessment COIS- Human subjects: Informed consent for treatment and open access publication was obtained or waived by all participants in this study. Institutional Review Board of RVS Dental College, Coimbatore, India issued approval 129/ETHICS/2025. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/09/27 CRDT- 2026/10/28 05:53 PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:53 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - 10.7759/cureus.117015 [doi] PST - epublish SO - Cureus. 2026 Sep 27;18(9):e117015. doi: 10.7759/cureus.117015. eCollection 2026 Sep. PMID- 42948835 OWN - NLM STAT- Publisher LR - 20261029 IS - 2475-0360 (Electronic) IS - 2475-0360 (Linking) DP - 2026 Oct 27 TI - Blood-Based Biomarkers in Alzheimer's Disease: Advances in Detection Technologies and Clinical Applications. LID - 10.1002/agm2.70112 [doi] AB - Alzheimer's disease (AD) is a neurodegenerative disorder. Irreversible pathological alterations are already present when clinical symptoms emerge. Therefore, early diagnosis and intervention are crucial for improving patient outcomes and reducing the social and healthcare burden. With its advantages of minimal invasiveness, convenience, affordability, and high accessibility, blood-based testing has overcome the limitations of conventional methods, becoming a major focus of research in early AD diagnosis in recent years. Based on the key domestic and international research findings in recent years, this paper systematically reviews the types, mechanisms of action, and relevant detection technologies of blood-based biomarkers for AD. CI - (c) 2026 The Author(s). Aging Medicine published by Beijing Hospital and John Wiley & Sons Australia, Ltd. FAU - Li, Shuangshuang AU - Li S AD - School of International Medical Technology Shanghai Sanda University Shanghai China. FAU - Huang, Lin AU - Huang L AD - Department of Gerontology Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai China. FAU - Guo, Qihao AU - Guo Q AUID- ORCID: 0000-0001-5079-8047 AD - School of International Medical Technology Shanghai Sanda University Shanghai China. AD - Department of Gerontology Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai China. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Australia TA - Aging Med (Milton) JT - Aging medicine (Milton (N.S.W)) JID - 101741954 PMC - PMC13713016 OTO - NOTNLM OT - Alzheimer disease OT - blood biomarkers OT - detection technologies OT - early diagnosis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/27 CRDT- 2026/10/28 05:53 PHST- 2026/04/06 00:00 [received] PHST- 2026/07/07 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:53 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - AGM270112 [pii] AID - 10.1002/agm2.70112 [doi] PST - aheadofprint SO - Aging Med (Milton). 2026 Oct 27:10.1002/agm2.70112. doi: 10.1002/agm2.70112. PMID- 42948804 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-8021 (Print) IS - 1664-8021 (Electronic) IS - 1664-8021 (Linking) VI - 17 DP - 2026 TI - Perioperative immunogenomics and clinical outcomes under ERAS-based nursing care in muscle-invasive bladder cancer. PG - 1967034 LID - 10.3389/fgene.2026.1967034 [doi] LID - 1967034 AB - BACKGROUND: Surgical trauma is associated with perioperative immune perturbation in muscle-invasive bladder cancer (MIBC). Enhanced Recovery After Surgery (ERAS) combines multidisciplinary perioperative management with structured nursing care, but its immunogenomic associations remain incompletely characterized. METHODS: This retrospective historical-control cohort included 126 MIBC patients undergoing radical cystectomy with Bricker ileal conduit (ERAS-based care, n = 63; conventional care, n = 63). Serial peripheral immune profiling was performed at T0 (preoperative), T1 (24 h postoperative), and T2 (POD7/discharge). Tumor analyses included multiplex immunofluorescence, tumor mutational burden (TMB), and NanoString immune-gene profiling; spatial transcriptomics was performed in 24 specimens (12/group). Clinical outcomes included 30-day infectious complications and survival, while biomarker and immunogenomic analyses were considered exploratory. RESULTS: ERAS-based care was associated with a less pronounced postoperative CD8(+) T-cell decline (22.8% vs. 41.6%, P < 0.001) and lower T1 serum IL-6 (43.2 vs. 87.4 pg/mL, P < 0.001). An exploratory four-marker T1 immune index (CD8(+) count, IL-6, sPD-L1, and NK activity) showed predictive performance for 30-day infectious complications (AUC = 0.84, 95% CI 0.76-0.92). In resection specimens, the ERAS cohort had a higher proportion of the immune-inflamed tumor immune microenvironment (TIME) phenotype (52.4% vs. 28.6%, P = 0.012). Exploratory survival analyses also identified associations with an eight-gene immune signature and TMB status; these findings require validation. CONCLUSION: In this retrospective cohort, ERAS-based perioperative nursing and multidisciplinary care were associated with differences in systemic immune recovery, postoperative outcomes, and cross-sectional tumor immune features. The proposed immune-marker and gene-signature findings are exploratory and require independent validation before clinical implementation. CI - Copyright (c) 2026 Xin, Guo, Zheng and Li. FAU - Xin, Lu-Lu AU - Xin LL AD - Department of Urology / Nursing Department, Cangzhou Central Hospital of Hebei Province, Cangzhou, Hebei, China. FAU - Guo, Jin-Jin AU - Guo JJ AD - Department of Urology / Nursing Department, Cangzhou Central Hospital of Hebei Province, Cangzhou, Hebei, China. FAU - Zheng, Xin-Ying AU - Zheng XY AD - Department of Urology / Nursing Department, Cangzhou Central Hospital of Hebei Province, Cangzhou, Hebei, China. FAU - Li, Zhao-Yang AU - Li ZY AD - Department of Urology / Nursing Department, Cangzhou Central Hospital of Hebei Province, Cangzhou, Hebei, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Genet JT - Frontiers in genetics JID - 101560621 PMC - PMC13713013 OTO - NOTNLM OT - bladder cancer OT - enhanced recovery after surgery OT - immune biomarkers OT - perioperative immunogenomics OT - perioperative nursing OT - tumor immune microenvironment COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/14 CRDT- 2026/10/28 05:51 PHST- 2026/08/13 00:00 [received] PHST- 2026/09/04 00:00 [revised] PHST- 2026/09/14 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:51 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1967034 [pii] AID - 10.3389/fgene.2026.1967034 [doi] PST - epublish SO - Front Genet. 2026 Oct 14;17:1967034. doi: 10.3389/fgene.2026.1967034. eCollection 2026. PMID- 42948792 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2229-3485 (Print) IS - 2229-5488 (Electronic) IS - 2229-3485 (Linking) VI - 17 IP - 4 DP - 2026 Oct-Dec TI - Authoring a literature review article: The prewriting QUEST of a beginner! PG - 235-238 LID - 10.4103/picr.picr_261_26 [doi] AB - Clinical research professionals, skilled in medical writing, verbal communication, and presentations, are keen to become authors of a review article. They are not aware of the challenging prewriting process of meticulous planning, critical appraisal of the literature, brainstorming to select a topic, and structuring the article. Prewriting journey of acquiring knowledge and skills is discussed here briefly. CI - Copyright: (c) 2026 Perspectives in Clinical Research. FAU - Bhatt, Arun AU - Bhatt A AD - Consultant, Clinical Research and Drug Development, Vadodara, Gujarat, India. LA - eng PT - Journal Article DEP - 20261015 PL - India TA - Perspect Clin Res JT - Perspectives in clinical research JID - 101551517 PMC - PMC13712633 OTO - NOTNLM OT - Author OT - prewriting OT - review article COIS- There are no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/01 CRDT- 2026/10/28 05:51 PHST- 2026/08/18 00:00 [received] PHST- 2026/08/22 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:51 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - PCR-17-235 [pii] AID - 10.4103/picr.picr_261_26 [doi] PST - ppublish SO - Perspect Clin Res. 2026 Oct-Dec;17(4):235-238. doi: 10.4103/picr.picr_261_26. Epub 2026 Oct 15. PMID- 42948790 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2168-8184 (Print) IS - 2168-8184 (Electronic) IS - 2168-8184 (Linking) VI - 18 IP - 9 DP - 2026 Sep TI - Intraportal Rupture of a Hepatic Hydatid Cyst Complicated by Arterioportal Fistula and Hepatic Artery Pseudoaneurysm. PG - e117020 LID - 10.7759/cureus.117020 [doi] LID - e117020 AB - A 71-year-old woman with known hepatic hydatid disease presented with fever, abdominal pain, vomiting, and jaundice. Magnetic resonance imaging (MRI) with MR cholangiopancreatography revealed rupture of a right hepatic hydatid cyst with intraportal hydatid dissemination associated with a hepatic artery pseudoaneurysm and an arterioportal fistula. The patient rapidly developed severe acute cholangitis with multiorgan failure. Endoscopic retrograde cholangiopancreatography (ERCP) allowed extraction of the hydatid material and biliary stenting, followed by selective endovascular embolization of the hepatic artery pseudoaneurysm. Despite combined endoscopic and interventional management, the patient progressed to refractory multiorgan failure and died. The present case illustrates a rare and life-threatening vascular complication of hepatic hydatid disease, emphasizing the importance of early imaging diagnosis and multidisciplinary management. In the present case, simultaneous portal and arterial involvement was associated with an unfavorable outcome. CI - Copyright (c) 2026, Khaissidi et al. FAU - Khaissidi, Kawtar AU - Khaissidi K AD - Department of Radiology, Hassan II University Hospital, Fez, MAR. FAU - Chaouche, Ismail AU - Chaouche I AD - Department of Radiology, Hassan II University Hospital, Fez, MAR. FAU - Ouazzani, Hajar AU - Ouazzani H AD - Department of Radiology, Hassan II University Hospital, Fez, MAR. FAU - Akammar, Amal AU - Akammar A AD - Department of Radiology, Hassan II University Hospital, Fez, MAR. FAU - El Bouardi, Nizar AU - El Bouardi N AD - Department of Radiology, Hassan II University Hospital, Fez, MAR. FAU - Haloua, Meriem AU - Haloua M AD - Department of Radiology, Hassan II University Hospital, Fez, MAR. FAU - Alami, Badreddine AU - Alami B AD - Department of Radiology, Hassan II University Hospital, Fez, MAR. FAU - Boubbou, Meryem AU - Boubbou M AD - Department of Radiology, Hassan II University Hospital, Fez, MAR. FAU - Maaroufi, Mustapha AU - Maaroufi M AD - Department of Radiology, Hassan II University Hospital, Fez, MAR. FAU - Alaoui Lamrani, Moulay Youssef AU - Alaoui Lamrani MY AD - Department of Radiology, Hassan II University Hospital, Fez, MAR. LA - eng PT - Case Reports PT - Journal Article DEP - 20260927 PL - United States TA - Cureus JT - Cureus JID - 101596737 PMC - PMC13712482 OTO - NOTNLM OT - arterioportal fistula OT - endovascular embolization OT - hepatic artery pseudoaneurysm OT - hepatic hydatid cyst OT - intraportal rupture COIS- Human subjects: Informed consent for treatment and open access publication was obtained or waived by all participants in this study. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/09/27 CRDT- 2026/10/28 05:51 PHST- 2026/09/10 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:51 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - 10.7759/cureus.117020 [doi] PST - epublish SO - Cureus. 2026 Sep 27;18(9):e117020. doi: 10.7759/cureus.117020. eCollection 2026 Sep. PMID- 42948781 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2468-6441 (Electronic) IS - 2468-6441 (Linking) VI - 10 IP - 10 DP - 2026 Oct TI - The Hidden Danger of Aortic Stenosis-Exercise-Induced Cardiac Arrest in a Young Man with Bicuspid Aortic Valve. PG - 414-418 LID - 10.1016/j.case.2026.06.006 [doi] AB - * BAoV is the most common congenital heart disease. * Underestimating AS using TTE is rare. * The consequences of underestimating severe AS can be fatal. * Extensive diagnostic workup is advised when symptoms and TTE findings are discordant. FAU - Ege Rasmussen, Lars AU - Ege Rasmussen L AD - Department of Cardiology, Aarhus University Hospital, Aarhus, Denmark. FAU - Frederiksen, Christian Alcaraz AU - Frederiksen CA AD - Department of Cardiology, Aarhus University Hospital, Aarhus, Denmark. FAU - Krusenstjerna-Hafstrom, Thomas AU - Krusenstjerna-Hafstrom T AD - Department of Cardiology, Aarhus University Hospital, Aarhus, Denmark. FAU - Vase, Henrik AU - Vase H AD - Department of Cardiology, Aarhus University Hospital, Aarhus, Denmark. LA - eng PT - Case Reports PT - Journal Article DEP - 20260822 PL - United States TA - CASE (Phila) JT - CASE (Philadelphia, Pa.) JID - 101700477 PMC - PMC13712474 OAB - [Figure: see text] OABL- eng OTO - NOTNLM OT - 4D MR Flow OT - Aortic Stenosis OT - Bicuspid aortic valve OT - Echocardiography OT - Invasive Heart Catheterization OT - Out-of-Hospital Cardiac Arrest EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/08/22 CRDT- 2026/10/28 05:50 PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:50 [entrez] PHST- 2026/08/22 00:00 [pmc-release] AID - S2468-6441(26)00152-0 [pii] AID - 10.1016/j.case.2026.06.006 [doi] PST - epublish SO - CASE (Phila). 2026 Aug 22;10(10):414-418. doi: 10.1016/j.case.2026.06.006. eCollection 2026 Oct. PMID- 42948776 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2042-8812 (Print) IS - 2042-8812 (Electronic) IS - 2042-8812 (Linking) VI - 2026 IP - 10 DP - 2026 Oct TI - Removal of a large maxillary sinus mucocele via Le Fort I osteotomy during orthognathic surgery: a case report. PG - rjag974 LID - 10.1093/jscr/rjag974 [doi] LID - rjag974 AB - Maxillary sinus mucoceles are uncommon expansile lesions resulting from impaired sinus drainage and mucus accumulation. We report a 19-year-old male with a large left maxillary sinus mucocele identified during planning for bimaxillary orthognathic surgery. Preoperative cone-beam computed tomography showed complete sinus occupation with extension into the nasal cavity and toward the nasopharynx. During the independently planned Le Fort I osteotomy, maxillary down-fracture provided direct access, allowing intact removal of the well-encapsulated lesion. Histopathological examination confirmed a mucocele. At 2-month follow-up, the patient had no nasal symptoms, and postoperative imaging demonstrated marked sinus re-aeration without radiographic evidence of residual or recurrent disease. When independently indicated, Le Fort I osteotomy may provide effective access for simultaneous management of selected extensive maxillary sinus mucoceles. CI - (c) The Author(s) 2026. Published by Oxford University Press and JSCR Publishing Ltd. FAU - Boylu, Dilara AU - Boylu D AUID- ORCID: 0000-0001-8364-3973 AD - Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Bolu Abant Izzet Baysal University, Karakoy Neighborhood, Mehmed Semseddin Street, Building No. 6, Golkoy Campus, 14030 Bolu, Turkey. FAU - Boylu, Omer Faruk AU - Boylu OF AUID- ORCID: 0000-0002-7096-9736 AD - Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Bolu Abant Izzet Baysal University, Karakoy Neighborhood, Mehmed Semseddin Street, Building No. 6, Golkoy Campus, 14030 Bolu, Turkey. LA - eng PT - Case Reports PT - Journal Article DEP - 20261027 PL - England TA - J Surg Case Rep JT - Journal of surgical case reports JID - 101560169 PMC - PMC13712509 OTO - NOTNLM OT - Le Fort I osteotomy OT - maxillary sinus OT - mucocele OT - orthognathic surgery OT - surgical removal COIS- The authors declare that they have no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/27 CRDT- 2026/10/28 05:50 PHST- 2026/08/29 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:50 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - rjag974 [pii] AID - 10.1093/jscr/rjag974 [doi] PST - epublish SO - J Surg Case Rep. 2026 Oct 27;2026(10):rjag974. doi: 10.1093/jscr/rjag974. eCollection 2026 Oct. PMID- 42948773 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2168-8184 (Print) IS - 2168-8184 (Electronic) IS - 2168-8184 (Linking) VI - 18 IP - 9 DP - 2026 Sep TI - Isolated Unilateral Proptosis as a Heralding Feature of Ipsilateral Temporal Dural Arteriovenous Fistula. PG - e117001 LID - 10.7759/cureus.117001 [doi] LID - e117001 AB - Dural arteriovenous fistulas (dAVFs) are rare intracranial vascular abnormalities that may present with varied and sometimes atypical clinical features. While proptosis is commonly associated with anterior cranial or cavernous sinus fistulas, it is unusual in posterior fossa dAVFs. We present the case of a 41-year-old man with progressive redness, swelling, and protrusion of the left eye over six months, without associated pain, diplopia, headache, or cranial nerve deficits. Ocular examination revealed preserved visual acuity and extraocular movements, with normal fundus findings. An initial diagnosis of a cavernous sinus fistula was considered. However, brain MRI with time-of-flight sequences revealed a left temporal dAVF with feeders from the external carotid artery and drainage into the vein of Labbe and transverse sinus, with a possible connection to the superior division of the middle cerebral artery. On clinical re-examination, a bruit was noted in the left occipitotemporal region. Doppler and transcranial Doppler studies confirmed mixed arterial and venous flow. The patient was referred for digital subtraction angiography and embolization. The symptoms of dAVFs vary depending on the shunt characteristics, location, and venous drainage pattern. Ocular signs are uncommon in noncavernous dAVFs. In this case, venous reflux through the vein of Labbe may explain the isolated proptosis. Management is guided by angioarchitecture and associated risk features, with high-risk fistulas requiring timely intervention. This case highlights the need to consider cranial dural AVFs in the differential diagnosis of isolated proptosis, as timely diagnosis and treatment are crucial. CI - Copyright (c) 2026, Bhandari et al. FAU - Bhandari, Vaibhav AU - Bhandari V AD - Department of Neurology, All India Institute of Medical Sciences, New Delhi, IND. FAU - Garg, Divyani AU - Garg D AD - Department of Neurology, All India Institute of Medical Sciences, New Delhi, IND. FAU - Agarwal, Ayush AU - Agarwal A AD - Department of Neurology, All India Institute of Medical Sciences, New Delhi, IND. FAU - Anand, Abhishek AU - Anand A AD - Department of Neurology, All India Institute of Medical Sciences, New Delhi, IND. FAU - Srivastava, Achal Kumar AU - Srivastava AK AD - Department of Neurology, All India Institute of Medical Sciences, New Delhi, IND. LA - eng PT - Case Reports PT - Journal Article DEP - 20260927 PL - United States TA - Cureus JT - Cureus JID - 101596737 PMC - PMC13712402 OTO - NOTNLM OT - brain arteriovenous malformations OT - dural arteriovenous fistula OT - orbital proptosis OT - posterior fossa arteriovenous fistula OT - unilateral proptosis COIS- Human subjects: Informed consent for treatment and open access publication was obtained or waived by all participants in this study. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/09/27 CRDT- 2026/10/28 05:50 PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:50 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - 10.7759/cureus.117001 [doi] PST - epublish SO - Cureus. 2026 Sep 27;18(9):e117001. doi: 10.7759/cureus.117001. eCollection 2026 Sep. PMID- 42948772 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-302X (Print) IS - 1664-302X (Electronic) IS - 1664-302X (Linking) VI - 17 DP - 2026 TI - Physiological and transcriptional responses of lactic acid bacteria to sub-inhibitory cadmium exposure. PG - 1914052 LID - 10.3389/fmicb.2026.1914052 [doi] LID - 1914052 AB - INTRODUCTION: Cadmium is a widespread environmental contaminant that can adversely impact microbial growth and metabolism. While the ability of Lactic acid bacteria (LAB) to bind and sequester cadmium has been extensively investigated, less is known about their physiological adaptation to sub-inhibitory cadmium exposure. METHODS: Following the initial determination of the minimum inhibitory concentration (MIC), four selected LAB strains were exposed to sub-inhibitory cadmium concentrations (1/8, 1/4 and 1/2 x their respective MIC values) and investigated using a combined physiological and transcriptional approach. Growth performance, culturability, viability, and acidification activity were assessed alongside the expression of cadA, cadD, mntH, and czcA, genes involved in metal transport, homeostasis, and detoxification, respectively. RESULTS: The results revealed marked strain-dependent differences in cadmium tolerance and adaptation. Even at sub-inhibitory concentrations, cadmium affected bacterial physiology by reducing growth and acidification activity. Limosilactobacillus reuteri showed the strongest reduction in relative growth, which decreased to 18.8% of the untreated control at (1/2) x MIC, while Nile Red fluorescence increased up to 5.61-fold, indicating pronounced alterations in the membrane lipid environment. LIVE/DEAD analyses demonstrated a concentration-dependent increase in the population of membrane-compromised cells, although proportional losses in cell viability did not always accompany reductions in culturability. Nile Red fluorescence analyses further revealed significant alterations in the cellular lipid environment, suggesting membrane remodeling as an important component of the adaptive response to cadmium exposure. Transcriptional analyses further showed heterogeneous expression patterns among strains, suggesting that adaptation to cadmium is regulated through strain-specific responses rather than a shared regulatory strategy. DISCUSSION: Overall, these findings indicate that LAB responses to cadmium involve strain-dependent physiological and transcriptional changes. The study highlights the importance of considering strain-specific traits when evaluating bacterial responses to metal stress and provides a basis for future investigations into the molecular mechanisms underlying adaptation to sub-lethal cadmium exposure. CI - Copyright (c) 2026 Vardanyan, Mancini, Bernardi, Filannino, Calabrese and De Angelis. FAU - Vardanyan, Diana AU - Vardanyan D AD - Department of Soil, Plant and Food Science, University of Bari Aldo Moro, Bari, Italy. FAU - Mancini, Leonardo AU - Mancini L AD - Department of Soil, Plant and Food Science, University of Bari Aldo Moro, Bari, Italy. FAU - Bernardi, Matteo AU - Bernardi M AD - Department of Soil, Plant and Food Science, University of Bari Aldo Moro, Bari, Italy. FAU - Filannino, Pasquale AU - Filannino P AD - Department of Soil, Plant and Food Science, University of Bari Aldo Moro, Bari, Italy. FAU - Calabrese, Francesco Maria AU - Calabrese FM AD - Department of Soil, Plant and Food Science, University of Bari Aldo Moro, Bari, Italy. FAU - De Angelis, Maria AU - De Angelis M AD - Department of Soil, Plant and Food Science, University of Bari Aldo Moro, Bari, Italy. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Microbiol JT - Frontiers in microbiology JID - 101548977 PMC - PMC13712412 OTO - NOTNLM OT - cadmium stress OT - gene regulation OT - heavy metal resistance OT - lactic acid bacteria OT - membrane lipid remodeling OT - physiological adaptation COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author PF declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:50 PHST- 2026/06/19 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:50 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fmicb.2026.1914052 [doi] PST - epublish SO - Front Microbiol. 2026 Oct 13;17:1914052. doi: 10.3389/fmicb.2026.1914052. eCollection 2026. PMID- 42948770 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2090-6846 (Print) IS - 2090-6854 (Electronic) IS - 2090-6854 (Linking) VI - 2026 DP - 2026 TI - Titanium Hypersensitivity as a Potential Contributor to Bronchopleural Fistula and Surgical Staple Extrusion Following Lung Cancer Surgery: A Case Report. PG - 1050765 LID - 10.1155/crpu/1050765 [doi] LID - 1050765 AB - INTRODUCTION: Titanium is widely regarded as a highly biocompatible material and is extensively used in surgical implants. Nevertheless, delayed hypersensitivity reactions are increasingly recognized and may act as potential contributing factors to implant-related complications. Thoracic manifestations remain exceptionally rare, and their impact on clinical decision-making is poorly described. CASE DESCRIPTION: A 65-year-old man, with chronic obstructive pulmonary disease (COPD), a 60 pack-year smoking history (former smoker since 2005) and severe gas transfer impairment, underwent video-assisted thoracoscopic right lower lobectomy for lung adenocarcinoma. The postoperative course was complicated by a prolonged air leak requiring surgical staple reinforcement and talc pleurodesis. Approximately 2 months after surgery, the patient developed invasive pneumococcal disease with Streptococcus pneumoniae bacteremia, which resolved following appropriate antimicrobial therapy. Several months later, the patient developed a bronchopleural fistula (BPF) with endobronchial extrusion of titanium staples, confirmed by computed tomography and bronchoscopy. Given the unusual presentation, allergy evaluation was performed. Patch testing demonstrated epicutaneous sensitization with positive reactions to titanium (IV) oxalate hydrate, cobalt (II) chloride hexahydrate, and manganese chloride. The allergy work-up had a direct therapeutic impact. Endoscopic closure with an Amplatzer device, a procedure documented for central BPFs, was initially considered but deliberately avoided because the considered device contains nitinol (a nickel-titanium alloy), which would expose the sensitized patient to additional titanium. Owing to the high surgical risk, conservative multidisciplinary management with close clinical and radiological surveillance was adopted. DISCUSSION: BPF is a recognized complication after lung resection and is inherently multifactorial. In this patient, established surgical, anatomical (right lower lobectomy), and patient-related comorbidity risk factors coexisted with documented titanium sensitization, suggesting that hypersensitivity may have acted as a potential contributing or modulating factor to impaired tissue integration and staple extrusion. This case highlights the diagnostic challenges of titanium hypersensitivity and the distinction between cutaneous sensitization and operative tissue allergy. Importantly, it illustrates how allergy assessment may directly influence therapeutic decision-making by avoiding further exposure to potentially allergenic metallic devices. Titanium hypersensitivity should therefore be considered as an incremental factor in selected patients with unexplained implant-related complications, even when conventional risk factors are present. CI - Copyright (c) 2026 Mariana Lucena et al. Case Reports in Pulmonology published by John Wiley & Sons Ltd. FAU - Lucena, Mariana AU - Lucena M AUID- ORCID: 0000-0003-0000-2607 AD - Allergy and Clinical Immunology Department Coimbra University Hospitals Local Health Unit of Coimbra Coimbra Portugal huc.min-saude.pt. FAU - Regalo, Sara AU - Regalo S AUID- ORCID: 0000-0003-3214-4140 AD - Allergy and Clinical Immunology Department Coimbra University Hospitals Local Health Unit of Coimbra Coimbra Portugal huc.min-saude.pt. FAU - Manso Preto, Leonor AU - Manso Preto L AUID- ORCID: 0000-0001-6217-4901 AD - Allergy and Clinical Immunology Department Coimbra University Hospitals Local Health Unit of Coimbra Coimbra Portugal huc.min-saude.pt. FAU - Todo Bom, Ana AU - Todo Bom A AUID- ORCID: 0000-0002-1850-6689 AD - Allergy and Clinical Immunology Department Coimbra University Hospitals Local Health Unit of Coimbra Coimbra Portugal huc.min-saude.pt. AD - Institute of Immunology Faculty of Medicine University of Coimbra Coimbra Portugal uc.pt. FAU - Regateiro, Frederico AU - Regateiro F AUID- ORCID: 0000-0002-6332-3056 AD - Allergy and Clinical Immunology Department Coimbra University Hospitals Local Health Unit of Coimbra Coimbra Portugal huc.min-saude.pt. AD - Institute of Immunology Faculty of Medicine University of Coimbra Coimbra Portugal uc.pt. AD - Center for Innovative Biomedicine and Biotechnology (CIBB) Faculty of Medicine University of Coimbra Coimbra Portugal uc.pt. AD - UBIAir-Clinical and Experimental Lung Center and CICS-UBI Health Sciences Research Cente University of Beira Interior Covilha Portugal ubi.pt. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Case Rep Pulmonol JT - Case reports in pulmonology JID - 101585355 PMC - PMC13712446 COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/27 CRDT- 2026/10/28 05:50 PHST- 2026/08/07 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:50 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - CRPU1050765 [pii] AID - 10.1155/crpu/1050765 [doi] PST - epublish SO - Case Rep Pulmonol. 2026 Oct 27;2026:1050765. doi: 10.1155/crpu/1050765. eCollection 2026. PMID- 42948766 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2211-3835 (Print) IS - 2211-3843 (Electronic) IS - 2211-3835 (Linking) VI - 16 IP - 10 DP - 2026 Oct TI - In vivo validation of intramolecular bivalent glues targeting PSMA reveals potent and tissue-selective BRD4 degradation in prostate cancer. PG - 6905-6923 LID - 10.1016/j.apsb.2026.08.008 [doi] AB - Various approaches have been explored to address the on-target, off-tissue toxicity of PROTACs and molecular glues, but most fall short in achieving robust in vivo selectivity and efficacy. In this study, we introduce a delivery strategy that enables tissue-selective deployment of an ultra-potent, picomolar-active DCAF16-recruiting intramolecular bivalent glue (IBG3) for BRD4 degradation in prostate cancer models by targeting the prostate-specific membrane antigen (PSMA). We first introduced rational modifications to IBG3 to install a suitable conjugation handle for PSMA ligand attachment, and incorporated a cathepsins-sensitive cleavable linker together with a dual functional polycarboxylate linker that enhances PSMA binding while suppressing passive diffusion. Selective BRD4 degradation was validated by multiple orthogonal assays in vitro and in vivo. The lead conjugate IBG-P3 achieved 79.8% tumor growth inhibition at 0.01 mg/kg in PC3-PIP xenografts, while an epimeric control lacking PSMA binding was inactive. Importantly, robust antitumor efficacy was further confirmed in an endogenous PSMA-expressing LNCaP xenograft model, where IBG-P3 achieved 72.6% tumor growth inhibition at a dose as low as 0.001 mg/kg, significantly outperforming the parent compound IBG3. This study provides the first in vivo validation of IBG degrader and establishes a preclinical framework for PSMA-guided delivery of potent degraders. CI - (c) 2026 The Authors. FAU - Cai, Deqin AU - Cai D AD - Lachman Institute for Pharmaceutical Development, School of Pharmacy, University of Wisconsin-Madison, WI 53705, USA. FAU - Li, Chunrong AU - Li C AD - Lachman Institute for Pharmaceutical Development, School of Pharmacy, University of Wisconsin-Madison, WI 53705, USA. FAU - Idrissou, Malick Bio AU - Idrissou MB AD - Department of Medical Physics, University of Wisconsin School of Medicine and Public Health, WI 53792, USA. FAU - Hawkins, Nina J AU - Hawkins NJ AD - Lachman Institute for Pharmaceutical Development, School of Pharmacy, University of Wisconsin-Madison, WI 53705, USA. FAU - Mudududdla, Ramesh AU - Mudududdla R AD - Lachman Institute for Pharmaceutical Development, School of Pharmacy, University of Wisconsin-Madison, WI 53705, USA. FAU - Chen, Xuankun AU - Chen X AD - Lachman Institute for Pharmaceutical Development, School of Pharmacy, University of Wisconsin-Madison, WI 53705, USA. FAU - Nguyen, Thao-Vy T AU - Nguyen TT AD - Lachman Institute for Pharmaceutical Development, School of Pharmacy, University of Wisconsin-Madison, WI 53705, USA. FAU - Li, Xiaolei AU - Li X AD - Lachman Institute for Pharmaceutical Development, School of Pharmacy, University of Wisconsin-Madison, WI 53705, USA. FAU - Hernandez, Reinier AU - Hernandez R AD - Department of Medical Physics, University of Wisconsin School of Medicine and Public Health, WI 53792, USA. AD - Department of Radiology, University of Wisconsin School of Medicine and Public Health, WI 53792, USA. AD - Carbone Cancer Center, University of Wisconsin-Madison, WI 53792, USA. FAU - Tang, Weiping AU - Tang W AD - Lachman Institute for Pharmaceutical Development, School of Pharmacy, University of Wisconsin-Madison, WI 53705, USA. AD - Carbone Cancer Center, University of Wisconsin-Madison, WI 53792, USA. AD - Department of Chemistry, University of Wisconsin-Madison, WI 53706, USA. LA - eng PT - Journal Article DEP - 20260811 PL - Netherlands TA - Acta Pharm Sin B JT - Acta pharmaceutica Sinica. B JID - 101600560 PMC - PMC13712433 OTO - NOTNLM OT - BRD4 OT - Intramolecular bivalent glue OT - PDC OT - PSMA OT - Precision degradation OT - Prostate cancer COIS- A provisional patent related to this manuscript has been filed. Weiping Tang is the shareholder of Chimergen Therapeutics Inc., NovaCure Biosciences Holding Inc., MolTenon Biosciences Inc., and GlycoBridge Biosciences Inc. Reinier Hernandez is the Chief Technology Officer of Archeus Technologies and a co-founder and Director of RPT Labworks Inc. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/08/11 CRDT- 2026/10/28 05:50 PHST- 2025/07/22 00:00 [received] PHST- 2026/05/11 00:00 [revised] PHST- 2026/05/27 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:50 [entrez] PHST- 2026/08/11 00:00 [pmc-release] AID - S2211-3835(26)00534-4 [pii] AID - 10.1016/j.apsb.2026.08.008 [doi] PST - ppublish SO - Acta Pharm Sin B. 2026 Oct;16(10):6905-6923. doi: 10.1016/j.apsb.2026.08.008. Epub 2026 Aug 11. PMID- 42948752 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2211-3835 (Print) IS - 2211-3843 (Electronic) IS - 2211-3835 (Linking) VI - 16 IP - 10 DP - 2026 Oct TI - Machine learning-driven discovery of aniline phenylacetate derivatives as LasR-targeted biofilm inhibitors against Pseudomonas aeruginosa infections. PG - 6852-6874 LID - 10.1016/j.apsb.2026.03.048 [doi] AB - Pseudomonas aeruginosa biofilm infections pose a severe clinical challenge due to antibiotic resistance driven by quorum sensing (QS). As the master regulator of the P. aeruginosa QS hierarchy, LasR controls biofilm initiation and key virulence factors, establishing it as a high-value therapeutic target. Here, we developed a machine learning pipeline integrating graph neural networks, pharmacophore modeling, molecular docking and molecular dynamics, and determined that the aniline-phenylacetic acid derivative (compound 2g) is an effective LasR inhibitor. 2g demonstrated exceptional biofilm inhibition (IC(50) = 0.14 +/- 0.08 mumol/L) without bactericidal activity (MIC >256 mumol/L). and bound LasR with high affinity (K (D) = 38.97 mumol/L). Mechanistically, transcriptomics revealed selective suppression of LasR-regulated virulence pathways, reducing pyocyanin production and bacterial motility. Notably, 2g synergized with antibiotics in vivo, reducing effective doses of ciprofloxacin and tobramycin by 1000-fold and 500-fold, respectively, while potently suppressing resistance evolution. Pharmacokinetic profiling further demonstrated favorable oral bioavailability (26.8%) and a wide safety margin. This work establishes 2g as a machine learning-optimized LasR inhibitor with potent antibiofilm activity and synergistic antibiotic enhancement, offering a translatable strategy against multidrug-resistant P. aeruginosa infections. CI - (c) 2026 The Authors. FAU - Huang, Yujing AU - Huang Y AD - College of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China. AD - College of Pharmacy, Jinan University, Guangzhou 510632, China. FAU - Chen, Haoming AU - Chen H AD - College of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China. FAU - Wu, Jiamin AU - Wu J AD - College of Pharmacy, Jinan University, Guangzhou 510632, China. FAU - Huang, Xiaojie AU - Huang X AD - College of Pharmacy, Jinan University, Guangzhou 510632, China. FAU - Zhang, Yingxin AU - Zhang Y AD - College of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China. FAU - Ren, Anmin AU - Ren A AD - Department of Pharmacology, Joint Laboratory of Guangdong-Hong Kong Universities for Vascular Homeostasis and Diseases, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China. FAU - Wu, Xiao AU - Wu X AD - College of Pharmacy, Jinan University, Guangzhou 510632, China. FAU - Huang, Chengyin AU - Huang C AD - College of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China. FAU - Xie, Wenguan AU - Xie W AD - College of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China. FAU - Zu, Duntao AU - Zu D AD - College of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China. FAU - Shen, Congzhen AU - Shen C AD - College of Pharmacy, Jinan University, Guangzhou 510632, China. FAU - Xu, Feifei AU - Xu F AD - Institute for Safflower Industry Research, Key Laboratory of Xinjiang Phytomedicine Resource and Utilization (Ministry of Education), College of Pharmacy, Shihezi University, Shihezi 832003, China. FAU - Chang, Yiqun AU - Chang Y AD - College of Pharmacy, Jinan University, Guangzhou 510632, China. FAU - Yang, Liang AU - Yang L AD - Department of Pharmacology, Joint Laboratory of Guangdong-Hong Kong Universities for Vascular Homeostasis and Diseases, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China. FAU - Zhou, Haibo AU - Zhou H AD - Institute for Safflower Industry Research, Key Laboratory of Xinjiang Phytomedicine Resource and Utilization (Ministry of Education), College of Pharmacy, Shihezi University, Shihezi 832003, China. AD - College of Pharmacy, Jinan University, Guangzhou 510632, China. FAU - Sun, Pinghua AU - Sun P AD - Institute for Safflower Industry Research, Key Laboratory of Xinjiang Phytomedicine Resource and Utilization (Ministry of Education), College of Pharmacy, Shihezi University, Shihezi 832003, China. AD - College of Pharmacy, Jinan University, Guangzhou 510632, China. FAU - Xu, Jun AU - Xu J AD - College of Pharmacy, Jinan University, Guangzhou 510632, China. FAU - Liu, Jun AU - Liu J AD - College of Pharmacy, Jinan University, Guangzhou 510632, China. FAU - Li, Shengrong AU - Li S AD - Institute for Safflower Industry Research, Key Laboratory of Xinjiang Phytomedicine Resource and Utilization (Ministry of Education), College of Pharmacy, Shihezi University, Shihezi 832003, China. FAU - Zheng, Junxia AU - Zheng J AD - College of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China. AD - Institute for Safflower Industry Research, Key Laboratory of Xinjiang Phytomedicine Resource and Utilization (Ministry of Education), College of Pharmacy, Shihezi University, Shihezi 832003, China. LA - eng PT - Journal Article DEP - 20260402 PL - Netherlands TA - Acta Pharm Sin B JT - Acta pharmaceutica Sinica. B JID - 101600560 PMC - PMC13712431 OTO - NOTNLM OT - Aniline phenylacetate derivatives OT - Antibacterial synergist OT - Antibiotic resistant OT - Biofilm OT - LasR inhibitor OT - Machine learning OT - Pseudomonas aeruginosa OT - Quorum sensing (QS) COIS- The authors declare no competing interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/04/02 CRDT- 2026/10/28 05:49 PHST- 2025/10/29 00:00 [received] PHST- 2026/02/13 00:00 [revised] PHST- 2026/02/22 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:49 [entrez] PHST- 2026/04/02 00:00 [pmc-release] AID - S2211-3835(26)00208-X [pii] AID - 10.1016/j.apsb.2026.03.048 [doi] PST - ppublish SO - Acta Pharm Sin B. 2026 Oct;16(10):6852-6874. doi: 10.1016/j.apsb.2026.03.048. Epub 2026 Apr 2. PMID- 42948748 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2211-3835 (Print) IS - 2211-3843 (Electronic) IS - 2211-3835 (Linking) VI - 16 IP - 10 DP - 2026 Oct TI - Emerging insights into PPARalpha's role in lean metabolic dysfunction-associated steatotic liver disease: Implications for targeted therapies. PG - 6462-6506 LID - 10.1016/j.apsb.2026.06.029 [doi] AB - Lean metabolic dysfunction-associated steatotic liver disease (MASLD), defined as hepatic steatosis in individuals with normal BMI (<25 kg/m(2) in non-Asians; <23 kg/m(2) in Asians), is a clinically paradoxical phenotype in which patients face risks of progressive fibrosis, cardiovascular events, and liver-related mortality comparable to or exceeding obese counterparts. Visceral adiposity, dietary stressors, cholesterol overload, and genetic susceptibility involving PNPLA3 variants and PPARA polymorphisms synergistically disrupt hepatic lipid homeostasis. At the core of this dysregulation lies peroxisome proliferator-activated receptor alpha (PPARalpha), a master transcriptional regulator of hepatic fatty acid oxidation, ketogenesis, and anti-inflammatory signaling. PPARalpha suppression aries through convergent mechanisms, including aging-related RAGE signaling, fructose-mediated epigenetic silencing, gut‒liver axis disruption, and lipotoxicity, operating independently of systemic obesity. This review demonstrates that: (1) impaired PPARalpha activity underlies lean MASLD pathogenesis through transcriptional, epigenetic, and post-translational suppression; (2) synthetic PPARalpha agonists show limited applicability in this metabolically fragile population; and (3) natural product-derived modulators engage PPARalpha alongside FXR and LXR networks to restore metabolic balance. The PPARalpha-FGF21-ketogenesis axis and nuclear receptor crosstalk provide the mechanistic rationale for network-level therapeutic approaches. We advocate for spatially selective hepatic PPARalpha reactivation guided by lean-specific biomarker and genotype-stratified trial design. CI - (c) 2026 The Authors. FAU - Yang, Yafei AU - Yang Y AD - Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, National Quality Control Center for Donated Organ Procurement, Hubei Key Laboratory of Medical Technology on Transplantation, Hubei Provincial Clinical Research Center for Natural Polymer Biological Liver, Wuhan 430071, China. FAU - Zhang, Furong AU - Zhang F AD - Department of pharmacy, Maternal and Child Health Hospital of Hubei Province, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430070, China. FAU - Peng, Zhuang AU - Peng Z AD - Department of Pharmacology, Taikang Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan 430071, China. FAU - Wu, Yafei AU - Wu Y AD - Department of Pharmacology, Taikang Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan 430071, China. FAU - Li, Ling AU - Li L AD - Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, National Quality Control Center for Donated Organ Procurement, Hubei Key Laboratory of Medical Technology on Transplantation, Hubei Provincial Clinical Research Center for Natural Polymer Biological Liver, Wuhan 430071, China. FAU - Shen, Zixia AU - Shen Z AD - Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, National Quality Control Center for Donated Organ Procurement, Hubei Key Laboratory of Medical Technology on Transplantation, Hubei Provincial Clinical Research Center for Natural Polymer Biological Liver, Wuhan 430071, China. FAU - Ye, Qifa AU - Ye Q AD - Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, National Quality Control Center for Donated Organ Procurement, Hubei Key Laboratory of Medical Technology on Transplantation, Hubei Provincial Clinical Research Center for Natural Polymer Biological Liver, Wuhan 430071, China. FAU - Yue, Jiang AU - Yue J AD - Department of Pharmacology, Taikang Medical School (School of Basic Medical Sciences), Wuhan University, Wuhan 430071, China. FAU - Na, Shufang AU - Na S AD - Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University, National Quality Control Center for Donated Organ Procurement, Hubei Key Laboratory of Medical Technology on Transplantation, Hubei Provincial Clinical Research Center for Natural Polymer Biological Liver, Wuhan 430071, China. LA - eng PT - Journal Article PT - Review DEP - 20260618 PL - Netherlands TA - Acta Pharm Sin B JT - Acta pharmaceutica Sinica. B JID - 101600560 PMC - PMC13712298 OTO - NOTNLM OT - Drug therapy OT - Lean MASLD OT - Lipid metabolism OT - Metabolic disorders OT - Natural compounds OT - PPARalpha OT - Therapeutic target OT - Traditional herbal medicines COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/06/18 CRDT- 2026/10/28 05:49 PHST- 2025/12/22 00:00 [received] PHST- 2026/05/07 00:00 [revised] PHST- 2026/06/07 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:49 [entrez] PHST- 2026/06/18 00:00 [pmc-release] AID - S2211-3835(26)00385-0 [pii] AID - 10.1016/j.apsb.2026.06.029 [doi] PST - ppublish SO - Acta Pharm Sin B. 2026 Oct;16(10):6462-6506. doi: 10.1016/j.apsb.2026.06.029. Epub 2026 Jun 18. PMID- 42948739 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2075-0528 (Electronic) IS - 2075-051X (Print) IS - 2075-051X (Linking) VI - 26 IP - 1 DP - 2026 TI - Epidemiological, Clinicopathological, and Molecular Features of Melanoma in Oman: A single-institution study. PG - 538-547 LID - 10.18295/2075-0528.3014 [doi] AB - OBJECTIVES: Published data on melanoma in Oman are scarce. This study aimed to describe epidemiological, clinicopathological and molecular characteristics of melanoma patients in a single institution in Oman. METHODS: This retrospective study included patients with a histopathological diagnosis of invasive melanoma who were assessed at Sultan Qaboos Comprehensive Cancer Care and Research Centre, Muscat, Oman, between July 2021 and February 2025. Data on patient demographics, comorbidities, melanoma type, localisation, stage, key histopathological and molecular characteristics were extracted from electronic clinical charts and quantitatively described. RESULTS: A total of 31 patients were included. The median age at diagnosis was 52.9 years (interquartile range [IQR]: 41.6-66.0). Patients from the Middle East comprised 90.3% of the cohort (n = 28), while Western individuals accounted for 9.7% (n = 3). Cutaneous melanoma was the most frequent type, observed in 83.9% of cases (n = 26), followed by ocular melanoma in 6.5% (n = 2). Mucosal melanoma, central nervous system melanoma and melanoma of unknown primary site were each found in 3.2% of patients (n = 1 each). Among cutaneous melanomas, acral and skin non-acral subtypes were equally represented, each comprising 41.9% (n = 13). Staging data were available for 27 patients; Stage III was the most common at presentation (40.7%, n = 11), followed by Stage II (29.6%, n = 8) and Stages I and IV (14.8%, n = 4 each). The median tumour mutational burden (TMB) was 4.95 mutations per megabase (IQR: 3.86-6.4). Activating BRAF mutations were detected in 35.0% of tested patients (n = 7/20). Brisk tumour-infiltrating lymphocytes were present in 28.6% of evaluated cases (n = 4/14). CONCLUSIONS: Early-stage diagnosis was uncommon. Acral and sun-unexposed non-acral melanoma subtypes were highly prevalent and median TMB was low. These findings suggest that clinicopathological and molecular features of melanoma in Oman may differ from those in Western and Asia-Pacific countries, with potential implications for treatment and prevention strategies. CI - (c) The Author(s) 2026. FAU - Itkin, Boris AU - Itkin B AUID- ORCID: 0000-0001-8837-8427 AD - Department of Medical Oncology, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman. FAU - Niaz, Zahida AU - Niaz Z AUID- ORCID: 0000-0001-5230-4709 AD - Department of Pathology, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman. FAU - Mohammed, Khadiga AU - Mohammed K AUID- ORCID: 0000-0003-3343-9776 AD - Department of Radiation Oncology, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman. FAU - Ali, Tauseef AU - Ali T AUID- ORCID: 0009-0001-3579-4694 AD - Department of Radiation Oncology, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman. FAU - Tajammul, Syeda Sara AU - Tajammul SS AD - Department of Radiation Oncology, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman. FAU - Ahmad, Zubair AU - Ahmad Z AUID- ORCID: 0009-0000-9307-2852 AD - Department of Pathology, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman. FAU - Deshpande, Prashant Ajit AU - Deshpande PA AD - Department of Pathology, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman. FAU - Hanif, Muhammad Rashid AU - Hanif MR AUID- ORCID: 0009-0002-7284-5363 AD - Department of Medical Oncology, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman. FAU - Al Shaibi, Maha AU - Al Shaibi M AUID- ORCID: 0000-0003-0052-4229 AD - Department of Surgical Oncology, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman. FAU - Al Hosni, Mohammad AU - Al Hosni M AD - Department of Surgical Oncology, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman. LA - eng PT - Journal Article DEP - 20261023 PL - Oman TA - Sultan Qaboos Univ Med J JT - Sultan Qaboos University medical journal JID - 101519915 SB - IM MH - Humans MH - Oman/epidemiology MH - *Melanoma/epidemiology/pathology/genetics/diagnosis MH - Female MH - Male MH - Middle Aged MH - Retrospective Studies MH - Adult MH - Aged MH - Skin Neoplasms/epidemiology PMC - PMC13712362 OTO - NOTNLM OT - Melanoma OT - Middle East OT - Mutation OT - Neoplasm metastasis OT - Neoplasm staging OT - Oman OT - Skin neoplasms OT - Tumor biomarkers COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/23 CRDT- 2026/10/28 05:49 PHST- 2026/02/20 00:00 [received] PHST- 2026/09/02 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:49 [entrez] PHST- 2026/10/23 00:00 [pmc-release] AID - squmj_v26_n01_squmj8226 [pii] AID - 10.18295/2075-0528.3014 [doi] PST - epublish SO - Sultan Qaboos Univ Med J. 2026 Oct 23;26(1):538-547. doi: 10.18295/2075-0528.3014. eCollection 2026. PMID- 42948738 OWN - NLM STAT- Publisher LR - 20261029 IS - 0034-6535 (Print) IS - 1530-9142 (Electronic) IS - 0034-6535 (Linking) DP - 2026 Sep 2 TI - On Recoding Ordered Treatments as Binary Indicators. LID - 10.1162/rest_a_01462 [doi] AB - Researchers using instrumental variables to investigate ordered treatments often recode treatment into an indicator for any exposure. We investigate this estimand under the assumption that the instruments shift compliers from no treatment to some but not from some treatment to more. We show that when there are extensive margin compliers only (EMCO) this estimand captures a weighted average of treatment effects that can be partially unbundled into each complier group's potential outcome means. We also establish an equivalence between EMCO and a two-factor selection model and apply our results to study treatment heterogeneity in the Oregon Health Insurance Experiment. FAU - Rose, Evan K AU - Rose EK AD - Assistant Professor, Department of Economics, University of Chicago, and NBER. FAU - Shem-Tov, Yotam AU - Shem-Tov Y AD - Assistant Professor, Department of Economics, University of California, Los Angeles. LA - eng GR - P2C HD041022/HD/NICHD NIH HHS/United States PT - Journal Article DEP - 20260902 PL - United States TA - Rev Econ Stat JT - The review of economics and statistics JID - 9877925 PMC - PMC13712300 MID - NIHMS2066937 OTO - NOTNLM OT - C01 OT - C26 EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/28 CRDT- 2026/10/28 05:49 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:49 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 10.1162/rest_a_01462 [doi] PST - aheadofprint SO - Rev Econ Stat. 2026 Sep 2:10.1162/rest_a_01462. doi: 10.1162/rest_a_01462. PMID- 42948727 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2168-8184 (Print) IS - 2168-8184 (Electronic) IS - 2168-8184 (Linking) VI - 18 IP - 9 DP - 2026 Sep TI - Fatal Fruit: Unveiling the Risks and Responses to Ackee Poisoning in a South Florida Inpatient Unit. PG - e117006 LID - 10.7759/cureus.117006 [doi] LID - e117006 AB - The ackee fruit is a staple in many Afro-Caribbean diets. Yet, when consumed in its unripe form, it can induce Jamaican vomiting sickness, a potentially fatal condition caused by hypoglycin, a toxin that disrupts fatty acid oxidation and gluconeogenesis. Although cases are well-documented in endemic regions, reports outside the Caribbean remain rare. We report the case of a 33-year-old male with no comorbidities who presented in South Florida with acute metabolic encephalopathy, severe hypoglycemia, and profound electrolyte disturbances following the ingestion of unripe ackee fruit. Despite early recognition and aggressive interventions, including glucose administration, supportive therapy, and management coordinated by internal medicine, neurology, nephrology, toxicology, intensive care, and palliative care teams, the patient's clinical condition continued to deteriorate over 21 days post-exposure. This case is distinctive for its occurrence in a non-endemic region, the absence of comorbidities, and the refractory course despite multidisciplinary intervention. It highlights both the diagnostic and therapeutic challenges of ackee poisoning in unfamiliar clinical settings. Increased globalization and the availability of culturally specific foods in diverse regions may increase the likelihood of similar presentations outside endemic areas. Awareness among healthcare providers is therefore essential. Ackee poisoning should be considered in patients presenting with unexplained hypoglycemia and encephalopathy, particularly in communities with Afro-Caribbean dietary practices. Early recognition, timely intervention, and interdisciplinary collaboration remain critical to improving patient outcomes. This case underscores the importance of provider awareness regarding culturally specific dietary exposures and their potential for severe toxicological consequences. CI - Copyright (c) 2026, Sears et al. FAU - Sears, Shalondria J AU - Sears SJ AD - College of Medicine, Florida Atlantic University Charles E. Schmidt College of Medicine, Boca Raton, USA. FAU - Gondar, Enrique AU - Gondar E AD - Internal Medicine, Broward Health Medical Center, Fort Lauderdale, USA. FAU - Sowden, Kevin AU - Sowden K AD - Internal Medicine, Broward Health Medical Center, Fort Lauderdale, USA. FAU - Aquino-Thomas, Jessene AU - Aquino-Thomas J AD - Invasive Plant Research Lab, US Department of Agriculture (USDA) Agricultural Research Lab, Fort Lauderdale, USA. FAU - Chokshi, Ruchir AU - Chokshi R AD - Internal Medicine, Broward Health Medical Center, Fort Lauderdale, USA. LA - eng PT - Case Reports PT - Journal Article DEP - 20260927 PL - United States TA - Cureus JT - Cureus JID - 101596737 PMC - PMC13712243 OTO - NOTNLM OT - ackee poison OT - acute metabolic encephalopathy OT - foodborne toxins OT - hypoglycin OT - ingestion of toxic materials OT - interdisciplinary care OT - jamaica OT - jamaican vomiting sickness OT - sudden early death OT - toxic plant COIS- Human subjects: Informed consent for treatment and open access publication was obtained or waived by all participants in this study. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/09/27 CRDT- 2026/10/28 05:48 PHST- 2024/09/11 00:00 [received] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:48 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - 10.7759/cureus.117006 [doi] PST - epublish SO - Cureus. 2026 Sep 27;18(9):e117006. doi: 10.7759/cureus.117006. eCollection 2026 Sep. PMID- 42948722 OWN - NLM STAT- Publisher LR - 20261029 IS - 2046-2069 (Electronic) IS - 2046-2069 (Linking) DP - 2026 Oct 27 TI - Development of 7-hydroxy-4-methylcoumarin-cyanoacrylate hybrids with antiproliferative activity against breast cancer cells: from molecular design to in vivo validation. LID - 10.1039/d6ra06101j [doi] AB - In the present study, a series of novel 6,8-dibromo-7-hydroxy-4-methylcoumarin-cyanoacrylate hybrids and their corresponding pyrazole derivatives were designed, synthesized, and evaluated for antiproliferative activity against breast cancer cells. Among the tested compounds, cyanoacrylate derivative 8 emerged as the most active analogue against MCF-7 cells (IC(50) = 5.9 +/- 0.4 microM) and retained antiproliferative activity against MDA-MB-231 cells (IC(50) = 11.7 +/- 0.4 microM). Compound 8 exhibited weaker cytotoxicity toward the non-cancerous MCF-10A and WI-38 cell lines (IC(50) = 46 +/- 3 and 37.4 +/- 1.3 microM, respectively), although the magnitude of selectivity was cell-context dependent. Mechanistic investigations in MCF-7 cells revealed pronounced G2/M cell-cycle arrest (7.57% to 32.24%), inhibition of tubulin polymerization (IC(50) = 4.7 +/- 0.2 microM), and EGFR kinase activity (IC(50) = 50 +/- 3 nM). Flow-cytometric analysis showed an increase in total apoptotic cells from 2.71% to 33.19%, while LDH release increased 2.88-fold, indicating concomitant loss of membrane integrity. qRT-PCR demonstrated upregulation of BAX (7.9-fold), caspase-9 (8.8-fold), and p53 (4.7-fold) and downregulation of BCL-2 (0.27-fold), with corresponding protein-level changes confirmed by ELISA, providing complementary evidence for activation of the intrinsic apoptotic pathway. Molecular docking provided structural hypotheses for potential interactions of compound 8 within the tubulin colchicine-binding region and EGFR ATP-binding site. In vivo, compound 8 significantly suppressed tumor burden in the Ehrlich ascites carcinoma (EAC) mouse model, reducing viable tumor-cell counts by up to 72.4% and ascitic tumor volume by 85.3%. Treatment also ameliorated tumor-associated hepatic and renal dysfunction, oxidative stress, and inflammatory alterations, with histopathological and immunohistochemical findings supporting tissue recovery. Collectively, these findings identify compound 8 as a promising antiproliferative and antitumor lead and support further optimization and evaluation of this scaffold in more clinically relevant breast cancer models. CI - This journal is (c) The Royal Society of Chemistry. FAU - El-Zend, Manar A AU - El-Zend MA AUID- ORCID: 0000-0001-9957-2188 AD - Department of Chemistry, Faculty of Science, Port Said University Port Said Egypt m.elzend@sci.psu.edu.eg ieldeen@yahoo.com Zeinab.elsayed@sci.psu.edu.eg. FAU - El-Deen, Ibrahim M AU - El-Deen IM AD - Department of Chemistry, Faculty of Science, Port Said University Port Said Egypt m.elzend@sci.psu.edu.eg ieldeen@yahoo.com Zeinab.elsayed@sci.psu.edu.eg. FAU - Abdelkarim, Zeinab AU - Abdelkarim Z AD - Department of Chemistry, Faculty of Science, Port Said University Port Said Egypt m.elzend@sci.psu.edu.eg ieldeen@yahoo.com Zeinab.elsayed@sci.psu.edu.eg. FAU - Alanazi, Ibrahim M AU - Alanazi IM AUID- ORCID: 0000-0003-4865-7215 AD - Department of Pharmacology and Toxicology, Faculty of Medicine, Umm Al-Qura University Makkah 21955 Saudi Arabia imanzi@uqu.edu.sa. FAU - Alshawwa, Samar Zuhair AU - Alshawwa SZ AUID- ORCID: 0000-0001-9232-7956 AD - Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University P.O. Box 84428 Riyadh 11671 Saudi Arabia SZAlshawwa@pnu.edu.sa. FAU - Saied, Essa M AU - Saied EM AUID- ORCID: 0000-0002-7342-6200 AD - Chemistry Department, Faculty of Science, Suez Canal University Ismailia 41522 Egypt essa.saied@science.suez.edu.eg. AD - Institute for Chemistry, Humboldt Universitat zu Berlin 12489 Berlin Germany. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - RSC Adv JT - RSC advances JID - 101581657 PMC - PMC13712327 COIS- The authors affirm that they are not aware of any personal or financial conflicts that would have seemed to affect the findings of this study's research. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/27 CRDT- 2026/10/28 05:48 PHST- 2026/07/07 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:48 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - d6ra06101j [pii] AID - 10.1039/d6ra06101j [doi] PST - aheadofprint SO - RSC Adv. 2026 Oct 27. doi: 10.1039/d6ra06101j. PMID- 42948708 OWN - NLM STAT- Publisher LR - 20261029 IS - 2376-6972 (Print) IS - 2376-6964 (Electronic) IS - 2376-6964 (Linking) DP - 2026 Oct 22 TI - Comparison of Intersectional Stigma Assessment Strategies for Black and Latino Gay, Bisexual, and Other Men Who Have Sex with Men. LID - 10.1037/sah0000726 [doi] AB - Discrimination, or enacted stigma, can negatively affect health behaviors and outcomes. Most studies measure enacted stigma related to membership in a single social category only, rather than accounting for the multiple stigmas people may experience. We used the validated Multiple Discrimination Scale (MDS) to compare intersectional stigma assessment strategies among Black and Latino gay, bisexual and other men who have sex with men (GBMSM; N = 658). Participants completed parallel MDS subscales assessing enacted stigma based on (a) race or ethnicity only, (b) sexual orientation only, and (c) the intersection of race or ethnicity and sexual orientation, alongside measures of mental and physical health, health behaviors, and medical mistrust. Regressions examined the predictive utility of (a) single (i.e., non-intersectional) stigma subscales (that assess racial or ethnic and sexual orientation discrimination in separate, parallel items), (b) interaction terms between single stigma subscales, and (c) an intersectional subscale (that assesses racial or ethnic and sexual orientation enacted stigma within the same items). Similar variance was explained across models for the association between enacted stigma and health outcomes, with single, non-intersectional stigma subscales explaining slightly more variance than the intersectional subscale. Interaction effects were generally non-significant, suggesting limited added value of statistical interaction terms when main effects are strong. The intersectional MDS subscale demonstrated high reliability and validity, offering a brief alternative to longer, single identity scales. Researchers could consider selecting stigma assessment strategies based on study goals and practical considerations, as different approaches yield comparable conclusions regarding stigma's health impacts. FAU - Bogart, Laura M AU - Bogart LM AUID- ORCID: 0000-0003-3182-5542 AD - RAND, Santa Monica, CA. AD - Charles R. Drew University of Medicine and Science, Dept. of Psychiatry, Los Angeles, CA. FAU - Mendoza-Graf, Alexandra AU - Mendoza-Graf A AD - RAND, Santa Monica, CA. FAU - Klein, David J AU - Klein DJ AD - RAND, Santa Monica, CA. FAU - Barreras, Joanna L AU - Barreras JL AD - California State University, Long Beach School of Social Work, Long Beach, CA. AD - Bienestar Human Services, Inc., Los Angeles, CA. FAU - Mutchler, Matt G AU - Mutchler MG AD - APLA Health, Community-Based Research, Los Angeles, CA. AD - California State University Dominguez Hills, School of Public Health and Health Sciences, Carson, CA. FAU - Brooks, Ronald A AU - Brooks RA AD - Bienestar Human Services, Inc., Los Angeles, CA. AD - University of California Los Angeles, Department of Family Medicine, David Geffen School of Medicine, Los Angeles, CA. FAU - Klinger, Ian A AU - Klinger IA AD - APLA Health, Community-Based Research, Los Angeles, CA. FAU - Robledo, Jesse AU - Robledo J AD - Bienestar Human Services, Inc., Los Angeles, CA. FAU - Lawrence, Sean J AU - Lawrence SJ AD - APLA Health, Community-Based Research, Los Angeles, CA. FAU - Cunningham, Emery V AU - Cunningham EV AD - Yale University. FAU - Pantalone, David W AU - Pantalone DW AD - Lehman College, School of Natural and Social Sciences, City University of New York, Bronx, NY. AD - The Fenway Institute, Fenway Health, Boston, MA. AD - University of Massachusetts Boston, Department of Psychology, Boston, MA. LA - eng GR - P30 MH058107/MH/NIMH NIH HHS/United States GR - R01 MD014722/MD/NIMHD NIH HHS/United States GR - R01 MH121256/MH/NIMH NIH HHS/United States PT - Journal Article DEP - 20261022 PL - United States TA - Stigma Health JT - Stigma and health JID - 101673746 PMC - PMC13712317 MID - NIHMS2201390 OTO - NOTNLM OT - Black OT - Discrimination OT - Intersectional Stigma OT - Latino OT - Men who have sex with men EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/28 CRDT- 2026/10/28 05:47 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:47 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 10.1037/sah0000726 [doi] PST - aheadofprint SO - Stigma Health. 2026 Oct 22:10.1037/sah0000726. doi: 10.1037/sah0000726. PMID- 42948704 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2211-3835 (Print) IS - 2211-3843 (Electronic) IS - 2211-3835 (Linking) VI - 16 IP - 10 DP - 2026 Oct TI - Association between the use of traditional Chinese medicine patterns as an inclusion criterion and statistical significance in primary outcomes of randomized placebo-controlled trials of Chinese herbal formulas. PG - 6664-6675 LID - 10.1016/j.apsb.2026.07.033 [doi] AB - Randomized, placebo-controlled trials of Chinese herbal formulas (CHFs) are commonly used to evaluate efficacy, but a proportion of such trials fail to demonstrate statistically significant effects on primary outcomes. Some researchers have pointed out that excluding traditional Chinese medicine (TCM) pattern differentiation from inclusion criteria may reduce the alignment between treatment and enrolled patient characteristics, which could, in turn, affect observed efficacy. This study analyzed 378 randomized, placebo-controlled trials of CHFs with clearly defined primary outcomes that were published between January 1, 2020, and October 21, 2025, and identified through searches of eight databases. A total of 92,691 participants were included in the primary outcome analyses. Of these, 324 trials (85.7%) reported statistically significant results on primary outcomes, and 197 (52.1%) used TCM patterns as an inclusion criterion. Multivariable Poisson regression revealed that trials using TCM patterns were more likely to report statistically significant results (adjusted risk ratio [aRR] = 1.14; 95% CI: 1.06-1.23; P = 0.001). The association was generally consistent across prespecified subgroups and sensitivity analyses; however, only 52 studies (13.8%) were evaluated as low risk of bias according to the Cochrane Risk of Bias 2.0 tool. Because this study included only published trials and few published pattern-based trials reported statistically nonsignificant primary outcomes, the observed association should be interpreted cautiously and may be influenced by publication bias, selective outcome reporting, and contextual differences in research and reporting practices. Further high-quality, prospectively registered, methodologically rigorous, and better-standardized trials are needed to evaluate this association. CI - (c) 2026 The Authors. FAU - Bu, Zhijun AU - Bu Z AD - The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, China. AD - Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China. FAU - Song, Meirong AU - Song M AD - The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, China. AD - Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China. FAU - Chen, Chen AU - Chen C AD - The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, China. FAU - Shi, Xinyi AU - Shi X AD - The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, China. AD - Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China. FAU - Wang, Xuefeng AU - Wang X AD - Centre for Evidence-Based Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China. FAU - Li, Yao AU - Li Y AD - The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, China. AD - Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China. FAU - Chong, Jinchen AU - Chong J AD - The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, China. AD - Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China. FAU - Zhu, Yijia AU - Zhu Y AD - The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, China. AD - Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China. FAU - Jiang, Feng AU - Jiang F AD - The Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, Nanjing 210029, China. AD - Department of Colorectal Surgery, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing 210029, China. FAU - Chen, Yugen AU - Chen Y AD - Department of Colorectal Surgery, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing 210029, China. AD - The Jiangsu Province Key Laboratory of Tumor Systems Biology and Chinese Medicine, Nanjing 210029, China. AD - The Jiangsu Collaborative Innovation Center of Chinese Medicine in Prevention and Treatment of Tumor, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China. LA - eng PT - Clinical Trial PT - Journal Article DEP - 20260725 PL - Netherlands TA - Acta Pharm Sin B JT - Acta pharmaceutica Sinica. B JID - 101600560 PMC - PMC13712285 OTO - NOTNLM OT - Chinese herbal formulas OT - Clinical trial methodology OT - Inclusion criteria OT - Pattern differentiation OT - Placebo-controlled trial OT - Primary outcomes OT - Statistical significance OT - Traditional Chinese medicine patterns COIS- The authors declare no competing interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/07/25 CRDT- 2026/10/28 05:47 PHST- 2026/02/22 00:00 [received] PHST- 2026/06/22 00:00 [revised] PHST- 2026/07/14 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:47 [entrez] PHST- 2026/07/25 00:00 [pmc-release] AID - S2211-3835(26)00474-0 [pii] AID - 10.1016/j.apsb.2026.07.033 [doi] PST - ppublish SO - Acta Pharm Sin B. 2026 Oct;16(10):6664-6675. doi: 10.1016/j.apsb.2026.07.033. Epub 2026 Jul 25. PMID- 42948702 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2211-3835 (Print) IS - 2211-3843 (Electronic) IS - 2211-3835 (Linking) VI - 16 IP - 10 DP - 2026 Oct TI - Lysosomal membrane damage repair and acidification impairment restoration triggered by smart lipid nanoparticles for Alzheimer's disease treatment. PG - 7037-7053 LID - 10.1016/j.apsb.2026.06.036 [doi] AB - Alzheimer's disease (AD), characterized by amyloid (Abeta) deposition in neurons, is the most serious neurodegenerative disorder, but lacks effective therapeutic drugs in the clinic. Impaired neuronal lysosomal function drives the pathological accumulation of intracellular Abeta. Targeted rehabilitation of lysosomal dysfunction may offer a potential strategy to restore neuronal homeostasis and mitigate AD progression. In this study, we developed a brain-targeted smart lipid nanoparticle (A-PSL@7BIO) to repair lysosomal membrane damage and restore impaired acidification in the brain for AD therapy. The system was composed of Angiopep-2 peptide-conjugated lipid nanoparticles, which were loaded with a Glycogen synthase kinase-3beta (GSK-3beta) inhibitor 7-bromoindirubin-3'-oxime (7BIO) and incorporated with dipalmitoyl-phosphatidylserine (DPPS) and a reactive oxygen species (ROS) responsive phospholipid. Through Angiopep-2-mediated blood-brain barrier (BBB) transcytosis, the smart lipid nanoparticles could enhance brain biodistribution with subsequent neuronal internalization. Pathologically elevated intracellular ROS levels in AD could induce stimulus-responsive payload release, wherein 7BIO selectively inhibited aberrant GSK-3beta hyperphosphorylation to restore the physiological acidic lysosomal environment and DPPS structurally repaired compromised lysosomal membranes, synergistically addressing dual aspects of lysosomal pathology. This smart lipid nanoparticles synergistically restored physiological lysosomal homeostasis, reversed neuronal dysfunction, and ameliorated cognitive deficits in AD models, indicating that A-PSL@7BIO could be used as a potential nanomedicine for AD therapy. CI - (c) 2026 The Authors. FAU - Yin, Jiaqing AU - Yin J AD - The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center of Nanjing Medical University; Pharmacy School of Nanjing Medical University, Nanjing 211166, China. AD - Jiangsu Province Innovation Center for Brain-Inspired Intelligence Technology, Nanjing 210000, China. FAU - Lv, Wei AU - Lv W AD - Department of Pharmacy, The Affiliated Jiangyin Clinical College of Xuzhou Medical University, Jiangyin 214400, China. FAU - Zhao, Wei AU - Zhao W AD - The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center of Nanjing Medical University; Pharmacy School of Nanjing Medical University, Nanjing 211166, China. AD - Jiangsu Province Innovation Center for Brain-Inspired Intelligence Technology, Nanjing 210000, China. FAU - Ding, Zhenwei AU - Ding Z AD - The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center of Nanjing Medical University; Pharmacy School of Nanjing Medical University, Nanjing 211166, China. AD - Jiangsu Province Innovation Center for Brain-Inspired Intelligence Technology, Nanjing 210000, China. FAU - Zhu, Fei AU - Zhu F AD - The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center of Nanjing Medical University; Pharmacy School of Nanjing Medical University, Nanjing 211166, China. FAU - Liu, Hongli AU - Liu H AD - The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center of Nanjing Medical University; Pharmacy School of Nanjing Medical University, Nanjing 211166, China. AD - Jiangsu Province Innovation Center for Brain-Inspired Intelligence Technology, Nanjing 210000, China. FAU - Li, Shengnan AU - Li S AD - The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center of Nanjing Medical University; Pharmacy School of Nanjing Medical University, Nanjing 211166, China. AD - Jiangsu Province Innovation Center for Brain-Inspired Intelligence Technology, Nanjing 210000, China. FAU - Cao, Xiang AU - Cao X AD - The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center of Nanjing Medical University; Pharmacy School of Nanjing Medical University, Nanjing 211166, China. AD - Jiangsu Province Innovation Center for Brain-Inspired Intelligence Technology, Nanjing 210000, China. FAU - Liu, Linfeng AU - Liu L AD - The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center of Nanjing Medical University; Pharmacy School of Nanjing Medical University, Nanjing 211166, China. AD - Jiangsu Province Innovation Center for Brain-Inspired Intelligence Technology, Nanjing 210000, China. FAU - Song, Kefan AU - Song K AD - The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center of Nanjing Medical University; Pharmacy School of Nanjing Medical University, Nanjing 211166, China. AD - Jiangsu Province Innovation Center for Brain-Inspired Intelligence Technology, Nanjing 210000, China. FAU - Gong, Yue AU - Gong Y AD - The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center of Nanjing Medical University; Pharmacy School of Nanjing Medical University, Nanjing 211166, China. AD - Jiangsu Province Innovation Center for Brain-Inspired Intelligence Technology, Nanjing 210000, China. FAU - Xu, Jiangna AU - Xu J AD - The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center of Nanjing Medical University; Pharmacy School of Nanjing Medical University, Nanjing 211166, China. AD - Jiangsu Province Innovation Center for Brain-Inspired Intelligence Technology, Nanjing 210000, China. FAU - Chen, Yuqin AU - Chen Y AD - The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center of Nanjing Medical University; Pharmacy School of Nanjing Medical University, Nanjing 211166, China. AD - Jiangsu Province Innovation Center for Brain-Inspired Intelligence Technology, Nanjing 210000, China. FAU - Zhang, Zhiyuan AU - Zhang Z AD - School of Basic Medical Sciences Nanjing Medical University, Nanjing 211166, China. FAU - Fang, Xiangming AU - Fang X AD - The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center of Nanjing Medical University; Pharmacy School of Nanjing Medical University, Nanjing 211166, China. FAU - Xin, Hongliang AU - Xin H AD - The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center of Nanjing Medical University; Pharmacy School of Nanjing Medical University, Nanjing 211166, China. AD - Jiangsu Province Innovation Center for Brain-Inspired Intelligence Technology, Nanjing 210000, China. LA - eng PT - Journal Article DEP - 20260622 PL - Netherlands TA - Acta Pharm Sin B JT - Acta pharmaceutica Sinica. B JID - 101600560 PMC - PMC13712284 OTO - NOTNLM OT - Alzheimer's disease OT - Blood-brain barrier OT - GSK-3 OT - Lysosomal dysfunction OT - Lysosomal repair OT - Neurodegeneration OT - Neuroinflammation OT - Smart lipid nanoparticles COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/06/22 CRDT- 2026/10/28 05:47 PHST- 2025/10/02 00:00 [received] PHST- 2026/02/01 00:00 [revised] PHST- 2026/02/18 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:47 [entrez] PHST- 2026/06/22 00:00 [pmc-release] AID - S2211-3835(26)00393-X [pii] AID - 10.1016/j.apsb.2026.06.036 [doi] PST - ppublish SO - Acta Pharm Sin B. 2026 Oct;16(10):7037-7053. doi: 10.1016/j.apsb.2026.06.036. Epub 2026 Jun 22. PMID- 42948694 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2211-3835 (Print) IS - 2211-3843 (Electronic) IS - 2211-3835 (Linking) VI - 16 IP - 10 DP - 2026 Oct TI - Post CDK4/6 inhibitors: Novel targeted combinations based on SERDs in HR(+)/HER2(-) advanced breast cancer. PG - 6378-6394 LID - 10.1016/j.apsb.2026.07.050 [doi] AB - The addition of cyclin-dependent kinase 4 and 6 (CDK4/6) inhibitors to endocrine therapy has redefined the treatment paradigm for HR-positive, HER2-negative (HR(+)/HER2(-)) advanced breast cancer, with clear survival gains. However, resistance is almost inevitable and remains the central barrier to durable benefit. Selective estrogen receptor degraders (SERDs) have therefore moved from a later-line option to a core component of post-progression strategies aimed at targeting ESR1 mutations and estrogen receptor (ER) pathway reactivation. Notably, oral SERDs differ substantially in ER occupancy, degradation potency, and pharmacokinetic behavior, and these differences appear to translate into distinct efficacy and toxicity profiles across studies. This review summarizes recent progress in SERD-based therapeutic strategies, with a focus on post-CDK4/6 inhibitor sequencing and biomarker-driven combination approaches. Particular attention is given to rational combinations with PI3Kalpha and AKT inhibitors, HER2-directed tyrosine kinase inhibitors, and emerging targets such as FGFR, CDK2, and CDK7, as well as the growing role of liquid biopsy in guiding treatment adaptation. Together, these developments position SERD-centered strategies as a key framework for advancing mechanism-informed, precision treatment in HR(+)/HER2(-) advanced breast cancer. CI - (c) 2026 The Authors. FAU - Wang, Yan AU - Wang Y AD - Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China. FAU - Wang, Jiayu AU - Wang J AD - Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China. FAU - Xu, Binghe AU - Xu B AD - Beijing Key Laboratory of Breast Cancer Innovative Drug Development and Translational Research, Beijing 100021, China. AD - Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China. LA - eng PT - Journal Article PT - Review DEP - 20260731 PL - Netherlands TA - Acta Pharm Sin B JT - Acta pharmaceutica Sinica. B JID - 101600560 PMC - PMC13712291 OTO - NOTNLM OT - Breast cancer OT - CDK4/6 inhibitor OT - Combination therapy OT - ESR1 mutations OT - Endocrine resistance OT - HR+/HER2- OT - PI3K/AKT pathway OT - Selective estrogen receptor degraders COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/07/31 CRDT- 2026/10/28 05:46 PHST- 2026/01/11 00:00 [received] PHST- 2026/04/12 00:00 [revised] PHST- 2026/04/29 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:46 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - S2211-3835(26)00511-3 [pii] AID - 10.1016/j.apsb.2026.07.050 [doi] PST - ppublish SO - Acta Pharm Sin B. 2026 Oct;16(10):6378-6394. doi: 10.1016/j.apsb.2026.07.050. Epub 2026 Jul 31. PMID- 42948691 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2211-3835 (Print) IS - 2211-3843 (Electronic) IS - 2211-3835 (Linking) VI - 16 IP - 10 DP - 2026 Oct TI - Mapping neutrophil membrane bioactivity to therapeutic interfaces: Biology, disease targeting, and activity-guided biomimetic therapy. PG - 6574-6605 LID - 10.1016/j.apsb.2026.08.013 [doi] AB - Neutrophil plasma membranes encode a tightly coordinated set of bioactivities that control inflammatory trafficking, endothelial adhesion under shear, effector activation, and immune regulation. In this review, we present a membrane-centric framework that conceptualizes the neutrophil membrane as a programmable therapeutic interface integrating targeting, adhesion, and effector regulation. We delineate core membrane modules-including chemokine-sensing G protein-coupled receptors (GPCRs), selectin ligands, beta2 integrins, Fcgamma and complement receptors, pattern-recognition receptors (PRRs), and lipid-raft microdomains-and examine how their coordinated dynamics govern tissue targeting, adhesion kinetics, effector thresholds, and resolution. Integrating evidence from infection, sterile inflammation, autoimmunity, and cancer, we highlight how dysregulated membrane programs drive pathology, while their selective tuning enables therapeutic benefit. Building on this biology, we discuss two convergent intervention strategies: direct pharmacological modulation of neutrophil membrane signaling axes, and activity-guided engineering of neutrophil membrane-derived therapeutics, including native vesicles and membrane-coated nanomaterials. We further link key engineering parameters-such as membrane orientation, ligand density, glycan architecture, and microdomain integrity-to mechanism-based potency, safety, and translational control. By positioning the neutrophil membrane as a programmable interface rather than a static biomimetic shell, this review outlines a rational roadmap for developing predictable, dose-controlled therapies for infection, inflammatory diseases, and cancer. CI - (c) 2026 The Authors. FAU - Yang, Qingqing AU - Yang Q AD - School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, China. FAU - Leng, Mingjing AU - Leng M AD - School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, China. FAU - Jiang, Wenbi AU - Jiang W AD - Yunnan Key Laboratory of Stem Cell and Regenerative Medicine, Rehabilitation School, Kunming Medical University, Kunming 650500, China. FAU - Yang, Huan AU - Yang H AD - Yunnan Key Laboratory of Stem Cell and Regenerative Medicine, Rehabilitation School, Kunming Medical University, Kunming 650500, China. FAU - Wu, Tong AU - Wu T AD - Yunnan Key Laboratory of Stem Cell and Regenerative Medicine, Rehabilitation School, Kunming Medical University, Kunming 650500, China. FAU - Zhou, Ting AU - Zhou T AD - Department of Neurology, Xinqiao Hospital, The Army Medical University (Third Military Medical University), Chongqing 400033, China. FAU - She, Zhennan AU - She Z AD - School of Pharmacy, Kunming Medical University, Kunming 650500, China. FAU - Xiang, Gang AU - Xiang G AD - School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, China. FAU - Zhou, Xing AU - Zhou X AD - Yunnan Key Laboratory of Stem Cell and Regenerative Medicine, Rehabilitation School, Kunming Medical University, Kunming 650500, China. FAU - Li, Xiaohui AU - Li X AD - Nuclear Medicine and Radiation Safety Key Laboratory of Sichuan Province, Major Disease Research & Translational Innovation Center, North Sichuan Medical College, Nanchong 637100, China. LA - eng PT - Journal Article PT - Review DEP - 20260826 PL - Netherlands TA - Acta Pharm Sin B JT - Acta pharmaceutica Sinica. B JID - 101600560 PMC - PMC13712293 OTO - NOTNLM OT - Activity-guided biomimetic therapy OT - Controlled release OT - Drug delivery OT - Engineering levers OT - Immunotherapy OT - Neutrophil membrane OT - Pharmacological modulation OT - Therapeutic interfaces COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/08/26 CRDT- 2026/10/28 05:46 PHST- 2025/11/17 00:00 [received] PHST- 2026/01/21 00:00 [revised] PHST- 2026/02/26 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:46 [entrez] PHST- 2026/08/26 00:00 [pmc-release] AID - S2211-3835(26)00541-1 [pii] AID - 10.1016/j.apsb.2026.08.013 [doi] PST - ppublish SO - Acta Pharm Sin B. 2026 Oct;16(10):6574-6605. doi: 10.1016/j.apsb.2026.08.013. Epub 2026 Aug 26. PMID- 42948688 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1178-6973 (Print) IS - 1178-6973 (Electronic) IS - 1178-6973 (Linking) VI - 19 DP - 2026 TI - Antibacterial Activity, Biofilm Inhibition, and Modulation of Virulence-Associated Gene Expression by Probiotic-Derived Cell-Free Supernatants Against Multidrug-Resistant Serratia marcescens. PG - 647738 LID - 10.2147/IDR.S647738 [doi] LID - 647738 AB - BACKGROUND: Multidrug-resistant (MDR) Serratia marcescens is an important opportunistic pathogen associated with persistent healthcare-associated infections, partly because of its capacity to form biofilms and express virulence-associated factors. Probiotic-derived cell-free supernatants (CFSs) contain secreted metabolites that may provide non-antibiotic approaches for controlling resistant bacteria. However, comparative data on bacterial- and yeast-derived CFSs against S. marcescens remain limited. METHODS: CFSs were prepared from Lacticaseibacillus rhamnosus GG, Bacillus coagulans, Bifidobacterium longum, and Saccharomyces boulardii. Metabolite profiles were examined by GC-MS. Antibacterial activity was assessed by disk diffusion and broth microdilution. Biofilm-formation inhibition was evaluated by crystal violet staining at 1/2 and 1/4 MIC. Expression of fimA, bsmA, and shlA was examined by qRT-PCR, and HEK293 cell viability was assessed by MTT. RESULTS: All four CFSs inhibited S. marcescens growth in a concentration-dependent manner. L. rhamnosus GG showed the greatest activity, with a MIC/MBC of 0.375/0.75 mg/mL and a 26-mm inhibition zone at 12 mg/mL. At 1/2 MIC, biofilm-formation inhibition was 71%, 57%, 47%, and 32%, respectively. All four CFSs significantly reduced fimA and bsmA expression, whereas shlA expression was significantly reduced by L. rhamnosus GG, B. coagulans, and B. longum, but not by S. boulardii. HEK293 cell viability remained above 80% under the tested conditions. CONCLUSION: Probiotic-derived CFSs demonstrated antibacterial and biofilm-formation inhibitory activities and were associated with reduced expression of selected virulence-associated genes in S. marcescens. L. rhamnosus GG-derived CFSs showed the strongest overall activity, supporting further mechanistic and in vivo studies. CI - (c) 2026 Asghari Ozma et al. FAU - Asghari Ozma, Mahdi AU - Asghari Ozma M AUID- ORCID: 0000-0002-4918-8820 AD - Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran. FAU - Fallahi Alileh, Niloofar AU - Fallahi Alileh N AD - Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. FAU - Moaddab, Seyyed Reza AU - Moaddab SR AD - Department of Laboratory Sciences, Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran. LA - eng PT - Journal Article DEP - 20261023 PL - New Zealand TA - Infect Drug Resist JT - Infection and drug resistance JID - 101550216 PMC - PMC13712229 OTO - NOTNLM OT - Serratia marcescens OT - antibacterial activity OT - antibiofilm activity OT - cell-free supernatants OT - multidrug-resistant bacteria COIS- The authors declare no competing interests. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/23 CRDT- 2026/10/28 05:46 PHST- 2026/08/11 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:46 [entrez] PHST- 2026/10/23 00:00 [pmc-release] AID - 647738 [pii] AID - 10.2147/IDR.S647738 [doi] PST - epublish SO - Infect Drug Resist. 2026 Oct 23;19:647738. doi: 10.2147/IDR.S647738. eCollection 2026. PMID- 42948687 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1178-2390 (Print) IS - 1178-2390 (Electronic) IS - 1178-2390 (Linking) VI - 19 DP - 2026 TI - Preoperative Neutrophil-to-Lymphocyte Ratio, Platelet-to-Lymphocyte Ratio, and Other Predictors of Delayed Wound Healing After Anal Fistula Surgery: A Retrospective Study. PG - 629310 LID - 10.2147/JMDH.S629310 [doi] LID - 629310 AB - OBJECTIVE: To evaluate the association of preoperative neutrophil-to-lymphocyte ratio (NLR) and platelet-to-lymphocyte ratio (PLR) with delayed wound healing after anal fistula surgery and to develop a combined risk prediction model. METHODS: Clinical data from 362 patients with anal fistula who underwent surgery between January 2022 and December 2024 were retrospectively analyzed. Patients were divided into a delayed healing group (>28 days, n=65) and a non-delayed healing group (600 degrees C), which promotes catalyst sintering and high energy demand, while at lower temperatures the competing Sabatier reaction dominates, producing CH(4) over Ni. Catalyst selection further complicates implementation: noble metals (Au, Pt, Rh) offer high CO selectivity but are costly, whereas base metals (Ni, Fe, Cu) are more abundant yet prone to methanation, sintering, or phase instability. Industrial deployment also demands resilience under water-rich environments, high space velocities, and thermal cycling associated with variable renewable H(2) supply. This Account presents our efforts to address these challenges through rational catalyst design guided by three central principles: electronic modulation via promoters to control adsorption and reaction pathways; active phase engineering to tune catalytic functionality and suppress undesired reactions, and structural and interfacial design to enhance stability and durability under realistic conditions. These principles are systematically explored using Ni- and Fe-based catalysts as model systems for scalable CO(2) valorization. Alkali-promoted Ni catalysts, particularly Cs-modified Ni/CeO(2), demonstrate how electronic tuning and metal-support interactions suppress methanation and enable CO-selective RWGS across a wide temperature range. Phase engineering further transforms Ni into phosphide intermetallic (Ni(2)P, Ni(12)P(5)), introducing active sites that favor CO formation while minimizing hydrogenation pathways. Complementary strategies in Fe-based systems, including MOF-derived Fe/carbon composites and Fe-Cu bimetallics, highlight the role of metal-support and metal-metal interactions in stabilizing active phases and enhancing CO yields. To address deactivation, nanoscale architectural control, such as core-shell and yolk-shell structures, has been employed to mitigate sintering and carbon deposition. Beyond single-function catalysts, dual-function and switchable materials integrate CO(2) capture with catalytic conversion and enable dynamic operation across RWGS, methanation, and dry reforming, providing flexibility under variable process conditions. Mechanistic insights derived from operando spectroscopy reveal how surface defects, promoters, and support redox properties govern reaction pathways, including formate-mediated and redox mechanisms, thereby linking catalyst structure to function. Collectively, these studies demonstrate how integrating electronic, compositional, and structural design strategies can reconcile the competing demands of activity, selectivity, and stability in RWGS catalysis. By bridging mechanistic understanding with practical engineering considerations, this work advances RWGS from a fundamental reaction toward a scalable platform for CO(2) valorization. These insights provide a framework for the design of next-generation catalytic materials and highlight the broader potential of rational materials engineering in enabling sustainable carbon utilization technologies. Looking ahead, operando-guided discovery, AI-assisted materials design, and integration with renewable energy infrastructures will be critical to realizing its full potential. CI - (c) 2026 The Authors. Co-published by ShanghaiTech University and American Chemical Society. FAU - Nawaz, Muhammad Asif AU - Nawaz MA AUID- ORCID: 0000-0003-3234-031X AD - Department of Inorganic Chemistry and Materials Science Institute, University of Seville-CSIC, 41092 Seville, Spain. FAU - Odriozola, Jose Antonio AU - Odriozola JA AUID- ORCID: 0000-0002-8283-0459 AD - Department of Inorganic Chemistry and Materials Science Institute, University of Seville-CSIC, 41092 Seville, Spain. AD - College of Environmental Science and Engineering, Beijing Forestry University, 35 Qinghua East Road, Haidian District, Beijing 100083, P.R. China. FAU - Reina, Tomas Ramirez AU - Reina TR AUID- ORCID: 0000-0001-9693-5107 AD - Department of Inorganic Chemistry and Materials Science Institute, University of Seville-CSIC, 41092 Seville, Spain. LA - eng PT - Journal Article DEP - 20260703 PL - United States TA - Acc Mater Res JT - Accounts of materials research JID - 101772849 PMC - PMC13711629 EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/07/03 CRDT- 2026/10/28 05:42 PHST- 2026/02/04 00:00 [received] PHST- 2026/06/04 00:00 [revised] PHST- 2026/06/12 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:42 [entrez] PHST- 2026/07/03 00:00 [pmc-release] AID - 10.1021/accountsmr.5c00275 [doi] PST - epublish SO - Acc Mater Res. 2026 Jul 3;7(10):1018-1033. doi: 10.1021/accountsmr.5c00275. eCollection 2026 Oct 23. PMID- 42948616 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2090-6900 (Print) IS - 2090-6919 (Electronic) IS - 2090-6919 (Linking) VI - 2026 DP - 2026 TI - Ductal Carcinoma In Situ With Microinvasion Arising in a Breast Hamartoma: A Case Report and Review of Guidelines of Hamartoma Management. PG - 1681410 LID - 10.1155/cris/1681410 [doi] LID - 1681410 AB - Breast hamartomas are rare, benign lesions composed of adipose, fibrous and glandular tissue. While typically non-malignant and not linked to increased breast cancer risk, they are often underdiagnosed due to variable imaging and histological features. We report a rare case of ductal carcinoma in situ (DCIS) with microinvasion arising within a mammary hamartoma. Unexpectedly the patient went on to develop a triple-negative invasive breast cancer 6 months following nipple-sparing mastectomy and implant reconstruction. To date, only 16 cases of breast carcinoma within hamartomas have been reported, making this case an important addition to the limited literature. It highlights the need for careful imaging interpretation, pathological review and multidisciplinary management, particularly in lesions with glandular components or atypical features. With increasing use of breast imaging, identification of hamartomas is expected to rise, making awareness of their rare malignant potential essential. In the absence of prevalence data or management guidelines, this case underscores the value of centralised reporting and collaborative clinical decision-making. CI - Copyright (c) 2026 Buket Ertansel et al. Case Reports in Surgery published by John Wiley & Sons Ltd. FAU - Ertansel, Buket AU - Ertansel B AUID- ORCID: 0000-0001-8388-569X AD - Department of Breast Surgery St. George's Hospital NHS Foundation Trust Blackshaw Road SW17 0QT London UK nhs.uk. FAU - Joseph, Geetha AU - Joseph G AUID- ORCID: 0009-0002-5886-172X AD - Department of Histopathology Kingston Hospital Kingston and Richmond NHS Foundation Trust Kingston Upon Thames KT2 7QB, Galsworthy Rd London UK kingstonhospital.nhs.uk. FAU - Syed, Oniaza AU - Syed O AUID- ORCID: 0009-0002-8610-3555 AD - Department of Clinical Radiology Kingston Hospital Kingston and Richmond NHS Foundation Trust Galsworthy Rd, Kingston Upon Thames KT2 7QB London UK kingstonhospital.nhs.uk. FAU - Kirby, Anna AU - Kirby A AUID- ORCID: 0009-0008-5673-0199 AD - Royal Marsden Institute of Cancer Research London UK icr.ac.uk. FAU - Parton, Marina AU - Parton M AUID- ORCID: 0000-0002-1407-4460 AD - Kingston Hospital Kingston and Richmond NHS Foundation Trust Galsworthy Rd, Kingston Upon Thames KT2 7QB London UK kingstonhospital.nhs.uk. FAU - Tang, Sarah Shuk-Kay AU - Tang SS AUID- ORCID: 0000-0002-9148-7525 AD - Department of Breast Surgery St. George's Hospital NHS Foundation Trust Blackshaw Road SW17 0QT London UK nhs.uk. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Case Rep Surg JT - Case reports in surgery JID - 101580191 PMC - PMC13711491 COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/27 CRDT- 2026/10/28 05:41 PHST- 2025/10/23 00:00 [received] PHST- 2026/09/07 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:41 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - CRIS1681410 [pii] AID - 10.1155/cris/1681410 [doi] PST - epublish SO - Case Rep Surg. 2026 Oct 27;2026:1681410. doi: 10.1155/cris/1681410. eCollection 2026. PMID- 42948614 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-2392 (Print) IS - 1664-2392 (Electronic) IS - 1664-2392 (Linking) VI - 17 DP - 2026 TI - Knowledge evolution and emerging emphasis on outcome standardization in diabetes remission research: a multidatabase bibliometric analysis, 1950 to January 2026. PG - 1943422 LID - 10.3389/fendo.2026.1943422 [doi] LID - 1943422 AB - BACKGROUND: Diabetes remission has evolved from an infrequently reported clinical observation into an increasingly defined and assessable outcome. Previous bibliometric studies have largely relied on the Web of Science and focused on literature published after 2000, limiting characterization of earlier knowledge foundations and long-term thematic change. This study mapped the global landscape and knowledge evolution of diabetes remission research and examined whether prespecified temporal boundaries were supported by annual publication trends. METHODS: The Web of Science Core Collection, Scopus, and PubMed were searched from database inception to January 30, 2026. After screening, deduplication, and metadata harmonization, 1,554 original articles were included. VOSviewer, CiteSpace, Bibliometrix, and SCImago Graphica were used to analyze publication trends, collaboration networks, journals, authors, keywords, thematic evolution, co-citation clusters, and citation bursts. Segmented Poisson regression was applied to annual publication counts from 1953 to 2025, with prespecified boundaries at 2000 and 2010 and BIC-based breakpoint selection. RESULTS: Among 1,543 articles published through 2025, the descriptive periods 1950-2000, 2001-2010, and 2011-2025 contained 188, 157, and 1,198 articles, respectively. The prespecified segmented model outperformed the no-breakpoint model (likelihood-ratio test, P < 0.001), with annual percentage changes of 5.19%, 13.00%, and 10.12%, respectively. The slope change after 2000 was significant (P < 0.001), whereas the additional change after 2010 was not (P = 0.115). The BIC-selected model identified one breakpoint at 2001.7 (95% CI, 1996.1-2007.4). The United States, China, and the United Kingdom were the leading contributors. Research themes evolved from case reports, animal studies, and transplantation toward metabolic surgery, weight loss, and lifestyle intervention, with increasing attention to remission definitions, durability, and long-term follow-up. CONCLUSIONS: Diabetes remission research evolved from documentation and foundational work through mechanistic development and evidence integration toward broader clinical evidence and increasing outcome standardization. Publication trends supported a major change in growth around 2000, whereas the 2011 boundary remained descriptive and was supported primarily by thematic rather than statistical evidence. CI - Copyright (c) 2026 Qi, Chen, Yang, Bi, Xu, Wang, Liu, Nie, Kang, Cheng, Zhang and Chen. FAU - Qi, Haotian AU - Qi H AD - Changchun University of Chinese Medicine, Changchun, China. FAU - Chen, Jinying AU - Chen J AD - Changchun University of Chinese Medicine, Changchun, China. AD - The Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, China. FAU - Yang, Han AU - Yang H AD - Changchun University of Chinese Medicine, Changchun, China. FAU - Bi, Ran AU - Bi R AD - Changchun University of Chinese Medicine, Changchun, China. AD - The Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, China. FAU - Xu, Shengxian AU - Xu S AD - Changchun University of Chinese Medicine, Changchun, China. FAU - Wang, Kuisong AU - Wang K AD - Changchun University of Chinese Medicine, Changchun, China. AD - The Third Affiliated Clinical Hospital of Changchun University of Chinese Medicine, Changchun, China. FAU - Liu, Zhenru AU - Liu Z AD - Changchun University of Chinese Medicine, Changchun, China. FAU - Nie, Weichen AU - Nie W AD - Changchun University of Chinese Medicine, Changchun, China. FAU - Kang, Xiaoxia AU - Kang X AD - Changchun University of Chinese Medicine, Changchun, China. FAU - Cheng, Siwen AU - Cheng S AD - Changchun University of Chinese Medicine, Changchun, China. FAU - Zhang, Xinlu AU - Zhang X AD - The Affiliated Hospital of Changchun University of Chinese Medicine, Changchun, China. FAU - Chen, Rui AU - Chen R AD - Changchun University of Chinese Medicine, Changchun, China. AD - College of Basic Medical Sciences, Changchun University of Chinese Medicine, Changchun, China. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Endocrinol (Lausanne) JT - Frontiers in endocrinology JID - 101555782 SB - IM MH - *Bibliometrics MH - Humans MH - Remission Induction MH - *Diabetes Mellitus/therapy PMC - PMC13711509 OTO - NOTNLM OT - bibliometrics OT - clinical translation OT - diabetes remission OT - knowledge evolution OT - research trends COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:41 PHST- 2026/07/20 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:41 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fendo.2026.1943422 [doi] PST - epublish SO - Front Endocrinol (Lausanne). 2026 Oct 13;17:1943422. doi: 10.3389/fendo.2026.1943422. eCollection 2026. PMID- 42948605 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-2295 (Electronic) IS - 1664-2295 (Linking) VI - 17 DP - 2026 TI - Advances in the treatment of intracranial blood blister-like aneurysms: a comprehensive review from surgical approaches to endovascular therapy. PG - 1952908 LID - 10.3389/fneur.2026.1952908 [doi] LID - 1952908 AB - Intracranial blood blister-like aneurysms (BBAs) constitute a rare but clinically challenging subset of cerebrovascular lesions, distinguished by their fragile, thin-walled morphology and a tendency to arise at non-branching sites of intracranial arteries, most frequently in the supraclinoid segment of the internal carotid artery (ICA). Managing BBAs is particularly difficult due to their high propensity for rupture, rapid progression, and the challenges they pose in intraoperative identification and repair. Traditional surgical approaches, including direct clipping, wrapping, and bypass with trapping, have been employed; however, these techniques are often associated with high complication rates and technical limitations given the delicate structure of BBAs. In recent years, advances in endovascular therapy, such as stent-assisted coiling (SAC) and flow-diverter (FD) placement, have expanded the therapeutic options, offering less invasive alternatives with encouraging outcomes. Nevertheless, the optimal management strategy remains controversial, as both surgical and endovascular approaches carry distinct indications, technical challenges, and risks of recurrence or procedural complications. This narrative review systematically explores the epidemiology and pathophysiology of BBAs, delineates key diagnostic features, and critically examines both reconstructive and deconstructive treatment modalities within a stepwise, two-pathway (endovascular and surgical) algorithm, highlighting reported efficacy, complication profiles, and recommended treatment selection for distinct aneurysm subtypes. By contrasting the strengths and limitations of each approach, this article aims to provide clinicians with evidence-based guidance to support individualized management strategies for patients with BBAs. CI - Copyright (c) 2026 Feng, Lei, Zhang, Li, Ou, Zhang and Yao. FAU - Feng, Ganghua AU - Feng G AD - Department of Neurology, The First People's Hospital of Chenzhou, Chenzhou, Hunan, China. AD - Department of Neurology, The First Clinical College of Xiangnan University, Chenzhou, Hunan, China. FAU - Lei, Yuanbiao AU - Lei Y AD - Department of Neurology, The First People's Hospital of Chenzhou, Chenzhou, Hunan, China. AD - Department of Neurology, The First Clinical College of Xiangnan University, Chenzhou, Hunan, China. FAU - Zhang, Yi AU - Zhang Y AD - Department of Neurology, The First People's Hospital of Chenzhou, Chenzhou, Hunan, China. AD - Department of Neurology, The First Clinical College of Xiangnan University, Chenzhou, Hunan, China. FAU - Li, Qiuli AU - Li Q AD - Department of Neurology, The First Affiliated Hospital & Clinical Neuroscience Institute of Jinan University, Guangzhou, Guangdong, China. FAU - Ou, Fuyong AU - Ou F AD - Department of Neurology, The First People's Hospital of Chenzhou, Chenzhou, Hunan, China. AD - Department of Neurology, The First Clinical College of Xiangnan University, Chenzhou, Hunan, China. FAU - Zhang, Le AU - Zhang L AD - Department of Neurology, The First People's Hospital of Chenzhou, Chenzhou, Hunan, China. AD - Department of Neurology, The First Clinical College of Xiangnan University, Chenzhou, Hunan, China. FAU - Yao, Xiaoxi AU - Yao X AD - Department of Neurology, The First People's Hospital of Chenzhou, Chenzhou, Hunan, China. AD - Department of Neurology, The First Clinical College of Xiangnan University, Chenzhou, Hunan, China. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Neurol JT - Frontiers in neurology JID - 101546899 SB - IM MH - Humans MH - *Intracranial Aneurysm/surgery MH - *Endovascular Procedures/methods MH - *Endovascular Aneurysm Repair/methods PMC - PMC13711506 OTO - NOTNLM OT - bypass OT - clipping OT - endovascular therapy OT - flow-diverter OT - intracranial blood blister-like aneurysm OT - surgical management COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:41 PHST- 2026/07/30 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:41 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fneur.2026.1952908 [doi] PST - epublish SO - Front Neurol. 2026 Oct 13;17:1952908. doi: 10.3389/fneur.2026.1952908. eCollection 2026. PMID- 42948598 OWN - NLM STAT- Publisher LR - 20261029 IS - 2769-2795 (Electronic) IS - 2313-1934 (Print) IS - 2313-1934 (Linking) DP - 2026 Oct 27 TI - Expert consensus on neuro-musculoskeletal functional reconstruction for post-stroke spastic hemiplegia. LID - 10.1002/ibra.70032 [doi] AB - Persistent motor dysfunction in post-stroke spastic hemiplegia may result from impaired voluntary motor control, abnormal muscle overactivity, fixed structural restriction, muscle force imbalance, or their combination. Advances in brain-computer interfaces (BCIs), peripheral nerve surgery, and tendon and soft-tissue reconstruction have expanded therapeutic options, yet optimal indications and sequencing remain unclear. A multidisciplinary expert panel reviewed current evidence, addressed key clinical questions, and developed consensus recommendations for noninvasive BCI, diagnostic motor nerve block (DNB), selective or hyperselective peripheral nerve surgery, and reconstructive procedures. The consensus emphasizes evaluation of four major components: central voluntary motor impairment, dynamic abnormal muscle overactivity, fixed structural limitations, and muscle force imbalance. Treatment should be guided by the patient's functional goals and the primary factor restricting performance. Task-oriented motor training remains fundamental when voluntary motor control is impaired, with BCI-based closed-loop training as a potential adjunct in selected patients. Focal, functionally significant muscle overactivity caused by abnormal neural drive may be considered for selective or hyperselective peripheral nerve surgery, with DNB assisting patient selection. Fixed structural restrictions may require tendon or soft-tissue procedures, while muscle force imbalance may benefit from tendon transfer or other dynamic reconstruction. When multiple impairments coexist, combined reconstruction followed by targeted motor retraining and comprehensive reassessment may be appropriate. Overall, neuro-musculoskeletal functional reconstruction should follow an individualized pathway linking functional goals, dominant pathophysiology, treatment targets, and outcomes. Given the variable evidence quality, no universal treatment sequence or combination strategy is currently supported. CI - (c) 2026 The Author(s). Ibrain published by Affiliated Hospital of Zunyi Medical University (AHZMU) and Wiley-VCH GmbH. FAU - Wen, Peng AU - Wen P AUID- ORCID: 0000-0003-1451-497X AD - Brain-Computer Interface Medical Center The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FAU - Wang, Yihe AU - Wang Y AUID- ORCID: 0000-0001-9705-9751 AD - Department of Neurosurgery, Xuanwu Hospital Capital Medical University Beijing China. FAU - He, Zhongzheng AU - He Z AD - Department of Neurosurgery Tianjin Huanhu Hospital Tianjin China. FAU - Zhu, Zhizhong AU - Zhu Z AD - Department of Rehabilitation Tianjin Huanhu Hospital Tianjin China. FAU - Chen, Chunbing AU - Chen C AD - Department of Radiology The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FAU - Wu, Kaifeng AU - Wu K AUID- ORCID: 0000-0002-8340-8866 AD - Department of Clinical Laboratory The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FAU - Fu, Xiaohong AU - Fu X AD - Brain-Computer Interface Medical Center The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FAU - Zhao, Guoguang AU - Zhao G AUID- ORCID: 0000-0002-7569-5252 AD - Department of Neurosurgery, Xuanwu Hospital Capital Medical University Beijing China. FAU - Xiong, Liulin AU - Xiong L AUID- ORCID: 0000-0002-1623-5969 AD - Brain-Computer Interface Medical Center The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. CN - "Zunyi Brain-Computer Interface Medical Center" Working Group LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - United States TA - Ibrain JT - Ibrain JID - 9918680988706676 PMC - PMC13711417 OTO - NOTNLM OT - brain-computer interface OT - expert consensus OT - functional reconstruction OT - spastic hemiplegia OT - stroke COIS- The authors declare no conflicts of interest. FIR - Cheng, Denggui IR - Cheng D IRAD- Department of Neurosurgery The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FIR - Deng, Zhaojian IR - Deng Z IRAD- Department of Neurosurgery The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FIR - Gong, Min IR - Gong M IRAD- Cerebrovascular Department The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FIR - Han, Chong IR - Han C IRAD- Cerebrovascular Department Affiliated Hospital of Zunyi Medical University Zunyi China. FIR - Jiang, Qijun IR - Jiang Q IRAD- Department of Neurosurgery The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FIR - Li, Hang IR - Li H IRAD- Cerebrovascular Department The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FIR - Li, Jianping IR - Li J IRAD- Neurocritical Care Unit The First People's Hospital of Zunyi [The Third Affiliated Hospital of Zunyi Medical University Zunyi China. FIR - Li, Shun IR - Li S IRAD- Department of Neurosurgery Affiliated Hospital of North Sichuan Medical College Nanchong China. FIR - Li, Yu IR - Li Y IRAD- Brain-Computer Interface Medical Center The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FIR - Luo, Minjie IR - Luo M IRAD- Department of Neurosurgery, Zhujiang Hospital Southern Medical University Guangzhou China. FIR - Ma, Chiyuan IR - Ma C IRAD- Department of Neurosurgery General Hospital of Eastern Theater Command Nanjing China. FIR - Shan, Yongzhi IR - Shan Y IRAD- Department of Neurosurgery, Xuanwu Hospital Capital Medical University Beijing China. FIR - Wang, Jun IR - Wang J IRAD- Department of Neurosurgery, Zhujiang Hospital Southern Medical University Guangzhou China. FIR - Wang, Nana IR - Wang N IRAD- Brain-Computer Interface Medical Center The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FIR - Wang, Qinglan IR - Wang Q IRAD- Brain-Computer Interface Medical Center The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FIR - Wang, Ziheng IR - Wang Z IRAD- Brain-Computer Interface Medical Center The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FIR - Wei, Zhongying IR - Wei Z IRAD- Brain-Computer Interface Medical Center The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FIR - Wu, Guojun IR - Wu G IRAD- Department of Neurology Yutian County Hospital of Traditional Chinese Medicine Hebei China. FIR - Xiong, Bo IR - Xiong B IRAD- Department of Neurology The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FIR - Xu, Jianguo IR - Xu J IRAD- Department of Neurosurgery, West China Hospital Sichuan University Chengdu China. FIR - Xu, Minpeng IR - Xu M IRAD- Haihe Laboratory of Brain-Computer Interaction and Human-Machine Integration Tianjin University Tianjin China. FIR - Yang, Kaihua IR - Yang K IRAD- Department of Neurosurgery The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FIR - Yang, Xi IR - Yang X IRAD- Brain-Computer Interface Medical Center The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FIR - Zhang, Fan IR - Zhang F IRAD- Department of Neurosurgery, West China Hospital Sichuan University Chengdu China. FIR - Zhang, Hongtian IR - Zhang H IRAD- Department of Neurosurgery The Seventh Medical Center of PLA General Hospital Beijing China. FIR - Zhang, Jing IR - Zhang J IRAD- Brain-Computer Interface Medical Center The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FIR - Zhang, Shaofu IR - Zhang S IRAD- Cerebrovascular Department The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FIR - Zhao, Hongxin IR - Zhao H IRAD- Cerebrovascular Department Affiliated Hospital of Zunyi Medical University Zunyi China. FIR - Zhao, Min IR - Zhao M IRAD- Department of Pharmacy The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. FIR - Zhu, Mingjiang IR - Zhu M IRAD- Brain-Computer Interface Medical Center The First People's Hospital of Zunyi (The Third Affiliated Hospital of Zunyi Medical University) Zunyi China. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/27 CRDT- 2026/10/28 05:40 PHST- 2026/09/03 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:40 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - IBRA70032 [pii] AID - 10.1002/ibra.70032 [doi] PST - aheadofprint SO - Ibrain. 2026 Oct 27:10.1002/ibra.70032. doi: 10.1002/ibra.70032. PMID- 42948595 OWN - NLM STAT- Publisher LR - 20261029 IS - 2046-2069 (Electronic) IS - 2046-2069 (Linking) DP - 2026 Oct 27 TI - Interface coupled MoS(2)@NiCoV-LDH heterostructures for efficient water splitting: synergistic synthesis and DFT insights. LID - 10.1039/d6ra07726a [doi] AB - Composition-controlled coupling of layered double hydroxides (LDHs) with transition metal dichalcogenides provides a pathway to bifunctional alkaline water-splitting electrodes based on earth-abundant elements. Here, trimetallic NiCoFe-, NiFeV- and NiCoV-LDHs were screened on nickel foam, and then the Ni : Co : V ratio was optimized and hydrothermally integrated with MoS(2) sequentially. The MoS(2)@Ni(0.25)Co(0.25)V(0.5)-LDH/NF electrode exhibited overpotentials of 50 mV for the oxygen evolution reaction (OER) and 114 mV for hydrogen evolution reaction (HER) at 10 mA cm(-2) with the Tafel slopes of 34.2 and 117 mV dec(-1), respectively. Electrochemical impedance measurements showed a decrease in interfacial charge-transfer resistance relative to the parent and inverse-growth electrodes. The DFT calculations predicted more electronic states near the Fermi level and modified the adsorption energetics of key reaction intermediates, which may provide evidence for the observed kinetic enhancement. The catalyst pair delivered 10 mA cm(-2) at approximately 1.66 V in a two-electrode system. These results highlight composition selection and growth sequence as coupled design parameters for the development of non-precious bifunctional electrodes for alkaline water splitting. CI - This journal is (c) The Royal Society of Chemistry. FAU - Amin, Youssef M I AU - Amin YMI AD - Chemical Engineering Department, Faculty of Engineering, The British University in Egypt El Sherouk City 11837 Egypt. FAU - Khalifa, Ziad AU - Khalifa Z AUID- ORCID: 0000-0002-9385-6857 AD - Chemical Engineering Department, Faculty of Engineering, The British University in Egypt El Sherouk City 11837 Egypt. FAU - Abdellatif, Sameh O AU - Abdellatif SO AD - The Electrical Engineering Department, FabLab, At the Centre of Emerging Learning Technologies CELT, British University in Egypt (BUE) 11387 Al Shorouk City Cairo Egypt. FAU - Kanoun, Olfa AU - Kanoun O AD - Professorship of Measurement and Sensor Technology, Chemnitz University of Technology Reichenhainer Str. 70 09126 Chemnitz Germany. FAU - Khalil, Ahmed S G AU - Khalil ASG AUID- ORCID: 0000-0003-1825-6752 AD - Environmental and Smart Technology Group, Faculty of Science, Fayoum University 63514 Fayoum Egypt asg05@fayoum.edu.eg. AD - EvoSmarTec GmbH Alfredstr. 81 45130 Essen Germany. AD - Institute of Basic and Applied Sciences, Egypt-Japan University of Science and Technology (E-JUST) New Borg El-Arab City 21934 Alexandria Egypt. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - RSC Adv JT - RSC advances JID - 101581657 PMC - PMC13711510 COIS- There are no conflicts to declare. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/27 CRDT- 2026/10/28 05:40 PHST- 2026/08/18 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:40 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - d6ra07726a [pii] AID - 10.1039/d6ra07726a [doi] PST - aheadofprint SO - RSC Adv. 2026 Oct 27. doi: 10.1039/d6ra07726a. PMID- 42948594 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1226-8453 (Print) IS - 2093-4947 (Electronic) IS - 1226-8453 (Linking) VI - 50 IP - 6 DP - 2026 Nov TI - Ginsenoside Rh2, a putative functional modulator of Annexin A2, attenuates post-hemorrhagic stroke cerebral edema via inhibiting vesicular transcytosis. PG - 101148 LID - 10.1016/j.jgr.2026.101148 [doi] LID - 101148 AB - BACKGROUND: Intracerebral hemorrhage (ICH) causes mechanical damage and cerebral edema. Annexin A2, expressed in endothelial cells, regulates cerebral edema, but its mechanisms in ICH remain unclear. METHODS: A mouse ICH model was established using collagenase VII-S. We measured Annexin A2 expression, transcytosis, and blood-brain barrier (BBB) permeability. AAV was used to specifically knock down Annexin A2 in cerebrovascular endothelial cells. Protein interactions were identified by co-immunoprecipitation and label-free LC-MS. Ginsenoside Rh2 was tested for effects on protein binding (biolayer interferometry and competition assays) and on neurological/motor function, BBB integrity, and transcytosis. RESULTS: ICH increased Annexin A2 expression, transcytosis, and BBB permeability. Annexin A2 knockdown worsened neurological deficits and motor function, increased BBB permeability, exacerbated tight junction disruption, but significantly reduced transcytosis. Six cytoskeletal proteins, Alpha-actinin-4, Actin-related protein 2, Actin alpha skeletal muscle, Actin cytoplasmic 1, Actin cytoplasmic 2, and Myosin-9, interacted with Annexin A2. Rh2 reduced binding affinity between Annexin A2 and these proteins, specifically inhibiting Annexin A2-Alpha-actinin-4 binding. Rh2 treatment improved neurological and motor function, reversed BBB disruption and tight junction damage, and suppressed transcytosis without altering Annexin A2 expression. CONCLUSION: Annexin A2 knockdown inhibited transcytosis but disrupted tight junctions and aggravated edema. In contrast, Rh2 inhibited transcytosis and exerts potential protective effect tight junctions, which may suggest a trend toward alleviation of cerebral edema to a certain extent. Selectively targeting Annexin A2-mediated transcytosis may be a promising therapy for ICH. CI - (c) 2026 The Korean Society of Ginseng. Publishing services by Elsevier B.V. FAU - Han, Xu AU - Han X AD - Department of Pathology and Pathophysiology, School of Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China. FAU - Li, Chu AU - Li C AD - Department of Pathology and Pathophysiology, School of Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China. FAU - Li, Qianlin AU - Li Q AD - Department of Physiology, School of Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China. FAU - You, Haixia AU - You H AD - Department of Pathology and Pathophysiology, School of Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China. FAU - Chen, Feiyan AU - Chen F AD - Research and Innovation Center, College of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China. FAU - Chen, Cuihua AU - Chen C AD - Research and Innovation Center, College of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China. FAU - Yao, Qi AU - Yao Q AD - Department of Pathology and Pathophysiology, School of Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China. FAU - Yang, Dongqing AU - Yang D AD - Department of Public Health, School of Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China. FAU - Chen, Lin AU - Chen L AD - Department of Physiology, School of Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China. FAU - Zhu, Zhu AU - Zhu Z AD - Department of Pathology and Pathophysiology, School of Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China. FAU - Zhao, Yunan AU - Zhao Y AD - Department of Pathology and Pathophysiology, School of Medicine, Nanjing University of Chinese Medicine, Nanjing, 210023, China. LA - eng PT - Journal Article DEP - 20260828 PL - Korea (South) TA - J Ginseng Res JT - Journal of ginseng research JID - 100890690 PMC - PMC13711444 OTO - NOTNLM OT - Annexin A2 OT - Blood-brain barrier OT - Cerebral edema OT - Ginsenoside Rh2 OT - Intracerebral hemorrhage OT - Transcytosis COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/08/28 CRDT- 2026/10/28 05:40 PHST- 2026/05/06 00:00 [received] PHST- 2026/07/08 00:00 [revised] PHST- 2026/08/21 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:40 [entrez] PHST- 2026/08/28 00:00 [pmc-release] AID - S1226-8453(26)00174-0 [pii] AID - 101148 [pii] AID - 10.1016/j.jgr.2026.101148 [doi] PST - ppublish SO - J Ginseng Res. 2026 Nov;50(6):101148. doi: 10.1016/j.jgr.2026.101148. Epub 2026 Aug 28. PMID- 42948585 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1226-8453 (Print) IS - 2093-4947 (Electronic) IS - 1226-8453 (Linking) VI - 50 IP - 6 DP - 2026 Nov TI - Fermentation of ginsenoside Rb1 by a probiotic mixture alleviates atopic dermatitis via synergistic effects of rare ginsenoside F2 and gut microbiota-derived 12-ketolithocholic acid in mice. PG - 101153 LID - 10.1016/j.jgr.2026.101153 [doi] LID - 101153 AB - Atopic Dermatitis (AD) is a chronic, relapsing inflammatory skin disorder with increasing global incidence. Emerging evidence highlights a strong association between AD and gut dysbiosis. In this context, compounds from Chinese herbal medicine (CHM), such as ginsenosides, have gained attention for their anti-inflammatory and immunomodulatory properties. However, the poor bioavailability of major ginsenosides limits their therapeutic effectiveness. Recent advances in probiotic-based herbal medicine fermentation suggest that, in addition to manipulation of gut dysbiosis, probiotics also show promising potential for enhancing the bioconversion of major ginsenosides into rare ginsenosides. The objective of this study was to determine whether probiotic-fermented ginsenoside Rb1 broth (Rb1FB) could alleviate 2,4-dinitrochlorobenzene (DNCB) atopic dermatitis-like disease and to investigate the associated gut-skin mechanisms. Rb1FB improved visible skin lesions and reduced dermatitis severity and significantly reduced epidermal thickening, dermal mast-cell infiltration, serum immunoglobulin E (IgE) and tumor necrosis factor-alpha (TNF-alpha) in AD mice. Rb1FB also increased gut microbial richness and diversity, enriched Bacteroides, increased fecal 12-ketolithocholic acid (12-KLCA), and enhanced systemic exposure to the rare ginsenoside F2. Therefore, the principal biological outcomes were improvements in the clinical-like, histopathological, allergic, and inflammatory features of AD, while the microbiome, metabolomic, and serum ginsenoside findings provided potential mechanistic explanations. METHODS: Probiotic strains Lactiplantibacillus plantarum DMU22441, Lactobacillus acidophilus DMU22440, and Lactiplantibacillus plantarum DMU22439 were screened for Rb1 utilization, and their fermentation conditions for maximum release of rare ginsenosides were optimized to prepare Rb1FB. A DNCB-induced AD mouse model was established to assess the therapeutic effects of Rb1FB. 16 S ribosomal RNA (rRNA) gene sequencing, metabolomics, transcriptomics and in vitro studies were employed to explore the mechanism of action. RESULTS: Rb1FB significantly alleviated AD symptoms in mice, reduced serum IgE and TNF-alpha levels, modulated the gut microbiota, and increased systemic exposure to the rare ginsenoside F2. In TNF-alpha-stimulated HaCaT cells, combined treatment with F2 and gut microbiota-associated 12-KLCA produced greater suppression of selected inflammatory genes than either treatment alone and was associated with reduced NUAK family kinase 2 (NUAK2). CONCLUSION: Rb1FB modulates the gut microbiota and its metabolites, enhances rare ginsenoside absorption, and alleviates AD symptoms in mice. F2 and 12-KLCA produced an enhanced combined inhibitory effect on inflammatory responses in vitro. These findings support the therapeutic potential of probiotic-fermented ginsenoside Rb1 for AD. CI - (c) 2026 The Korean Society of Ginseng. Publishing services by Elsevier B.V. FAU - Yuan, Donglin AU - Yuan D AD - Department of Dermatology, The Second Affiliated Hospital of Dalian Medical University, Dalian, 116000, China. FAU - Walayat, Bushra AU - Walayat B AD - College of Basic Medical Science, Dalian Medical University, Dalian, 116044, China. FAU - Chen, Xinyi AU - Chen X AD - Department of Dermatology, The Second Affiliated Hospital of Dalian Medical University, Dalian, 116000, China. FAU - Xiang, Dandan AU - Xiang D AD - College of Basic Medical Science, Dalian Medical University, Dalian, 116044, China. FAU - Xing, Yu AU - Xing Y AD - Department of Dermatology, The Second Affiliated Hospital of Dalian Medical University, Dalian, 116000, China. FAU - Wang, Chaoran AU - Wang C AD - Taizhou Medical City Guokehuawu Bio-Pharm Co., Ltd, Taizhou, 225300, China. FAU - Li, Ming AU - Li M AD - College of Basic Medical Science, Dalian Medical University, Dalian, 116044, China. FAU - Hou, Binbin AU - Hou B AD - Department of Dermatology, The Second Affiliated Hospital of Dalian Medical University, Dalian, 116000, China. LA - eng PT - Journal Article DEP - 20260911 PL - Korea (South) TA - J Ginseng Res JT - Journal of ginseng research JID - 100890690 PMC - PMC13711407 OTO - NOTNLM OT - 12-Ketolithocholic acid OT - Atopic dermatitis OT - Ginsenoside F2 OT - Gut microbiota OT - Probiotic fermentation COIS- The authors have declared no conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/09/11 CRDT- 2026/10/28 05:40 PHST- 2026/05/21 00:00 [received] PHST- 2026/08/05 00:00 [revised] PHST- 2026/09/08 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:40 [entrez] PHST- 2026/09/11 00:00 [pmc-release] AID - S1226-8453(26)00179-X [pii] AID - 101153 [pii] AID - 10.1016/j.jgr.2026.101153 [doi] PST - ppublish SO - J Ginseng Res. 2026 Nov;50(6):101153. doi: 10.1016/j.jgr.2026.101153. Epub 2026 Sep 11. PMID- 42948584 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2049-9442 (Electronic) IS - 2049-9434 (Print) IS - 2049-9434 (Linking) VI - 25 IP - 6 DP - 2026 Dec TI - Machine learning-based classification of renal cell carcinoma and upper tract urothelial carcinoma using preoperative hematological markers. PG - 139 LID - 10.3892/br.2026.2212 [doi] LID - 139 AB - Renal tumors are the second most common type of urological cancer. These tumors consist of renal cell carcinoma (RCC; 80-85%) and upper tract urothelial carcinoma (UTUC; 5-10%). Accurate preoperative differentiation between these two entities remains challenging despite advances in imaging and endoscopic techniques, often leading to potential misdiagnoses and inappropriate treatment strategies. Therefore, the development of novel diagnostic methods to improve preoperative diagnosis remains an area of notable clinical interest. At present, the potential for using machine learning (ML) based on preoperative hematological markers as non-invasive tools for cancer diagnosis and prognosis is being investigated. However, the application of ML based on preoperative hematological markers to differentiate RCC from UTUC remains unclear. The present study aimed to develop a classification system to differentiate between RCC and UTUC using ML based on preoperative hematological markers and clinical data from 172 patients with renal cancer, including 115 cases (66.9%) of RCC and 57 cases (33.1%) of UTUC. To address class imbalance, the Synthetic Minority Oversampling Technique was applied within a leakage-free pipeline restricted to the training data. Feature selection was performed using recursive feature elimination with cross-validation. A panel of ML algorithms was screened. The six best-performing models were tuned and evaluated, with performance assessed by accuracy, balanced accuracy, precision, recall, F1 score and the area under the receiver operating characteristic curve (AUC). The Extra Trees classifier showed the most favorable overall performance, with an accuracy of 75.0%, a balanced accuracy of 0.71 and an AUC value of 0.755 (bootstrap AUC, 0.71; 95% CI, 0.59-0.83). These preliminary findings suggest that ML models based on routinely available preoperative hematological markers may serve as a useful, low-cost adjunct to support the differentiation of RCC and UTUC. CI - Copyright: (c) 2026 Nuwatkrisin et al. FAU - Nuwatkrisin, Kanrapee AU - Nuwatkrisin K AD - Department of Surgery, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. FAU - Panwoon, Chanita AU - Panwoon C AD - Faculty of Medicine, Vongchavalitkul University, Nakhon Ratchasima 30000, Thailand. FAU - Senchan, Kiartisak AU - Senchan K AD - Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. FAU - Sangkhamanon, Sakkarn AU - Sangkhamanon S AD - Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. FAU - Seubwai, Wunchana AU - Seubwai W AD - Department of Forensic Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. AD - Center for Translational Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. LA - eng PT - Journal Article DEP - 20261009 PL - England TA - Biomed Rep JT - Biomedical reports JID - 101613227 PMC - PMC13711429 OTO - NOTNLM OT - hematological markers OT - machine learning OT - renal cell carcinoma OT - upper tract urothelial carcinoma COIS- The authors declare that they have no competing interests. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/09 CRDT- 2026/10/28 05:40 PHST- 2026/05/15 00:00 [received] PHST- 2026/09/02 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:40 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - BR-25-6-02212 [pii] AID - 10.3892/br.2026.2212 [doi] PST - epublish SO - Biomed Rep. 2026 Oct 9;25(6):139. doi: 10.3892/br.2026.2212. eCollection 2026 Dec. PMID- 42948578 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1226-8453 (Print) IS - 2093-4947 (Electronic) IS - 1226-8453 (Linking) VI - 50 IP - 6 DP - 2026 Nov TI - Magnetism-directed selective transformation of ginsenosides via Fe(3)O(4) nanozyme catalysis: Mechanism and green process development. PG - 101152 LID - 10.1016/j.jgr.2026.101152 [doi] LID - 101152 AB - BACKGROUND: Fe(3)O(4), as a peroxidase-mimicking metal nanozyme, has garnered broad attention across multiple disciplines. Ginsenosides, the primary bioactive constituents of the Araliaceae family, possess diverse pharmacological activities. Studies indicate that rare ginsenosides exhibit superior potency compared to their conventional counterparts. However, highcost and technical challenges of converting conventional ginsenosides into rare ones significantly limit their availability. OBJECTIVE: To fabricate a magnetic nanozyme for the highly selective conversion of the major ginsenoside Rb1 into the rare ginsenoside CK, and to elucidate the structure-activity relationship governing the product specificity. METHODS: Single-core Fe(3)O(4) nanoparticles were synthesized via a coprecipitation method, and their surfaces were subsequently modified with oleylamine/oleic acid to yield superparamagnetic iron oxide (SPIO) with weakened magnetism. Ginsenoside transformation was achieved using a standard emulsification procedure combined with ultrasonication and heating. Response surface methodology (RSM) was employed to optimize the transformation conditions. Product profiles were quantified by high-performance liquid chromatography (HPLC), and the adsorption mechanism was investigated through molecular dynamics (MD) simulations. RESULTS: Conversion experiments demonstrated that highly magnetic Fe(3)O(4) specifically converted Rb1 into a single product, the rare ginsenoside CK. In contrast, weakly magnetic SPIO produced a mixture predominantly composed of rare ginsenosides F2, CK, and Rh2. Response surface methodology (RSM) optimization yielded a maximum conversion rate of 77.54% at 49.53  degrees C, 3.35 h, and 28.48 kHz ultrasonic frequency. Molecular dynamics (MD) simulations further indicated that Fe(3)O(4) exhibited strong glycosyl binding competitiveness, preferentially adsorbing the glycosyl chain at the C-20 position of ginsenoside via chemisorption, potentially involving Fe-C coordination bond formation. CONCLUSION: Tuning the magnetic strength of Fe(3)O(4) nanozymes enables the selective transformation of Rb1 into CK, providing a promising green and efficient strategy for the scalable production of rare ginsenosides. CI - (c) 2026 The Korean Society of Ginseng. Publishing services by Elsevier B.V. FAU - Gao, Xinyu AU - Gao X AD - Qingdao University of Science and Technology, Qingdao, 266042, China. AD - Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266061, China. AD - Shandong Energy Institute, Qingdao, 266061, China. FAU - Ying, Huimin AU - Ying H AD - Qingdao University of Science and Technology, Qingdao, 266042, China. AD - Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266061, China. AD - Shandong Energy Institute, Qingdao, 266061, China. FAU - Zhang, Jiaming AU - Zhang J AD - Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266061, China. AD - Shandong Energy Institute, Qingdao, 266061, China. FAU - Zhang, Lina AU - Zhang L AD - Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266061, China. AD - Shandong Energy Institute, Qingdao, 266061, China. FAU - Huang, Xianbiao AU - Huang X AD - Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266061, China. AD - Shandong Energy Institute, Qingdao, 266061, China. FAU - Du, Yijin AU - Du Y AD - Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, 21205, USA. FAU - Wu, Xiaochen AU - Wu X AD - Qingdao University of Science and Technology, Qingdao, 266042, China. FAU - Yu, Changjiang AU - Yu C AD - Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266061, China. AD - Shandong Energy Institute, Qingdao, 266061, China. LA - eng PT - Journal Article DEP - 20260910 PL - Korea (South) TA - J Ginseng Res JT - Journal of ginseng research JID - 100890690 PMC - PMC13711441 OTO - NOTNLM OT - Catalytic conversion OT - Ginsenoside OT - Magnetic disparity OT - Metal nanoenzyme OT - Molecular dynamics (MD) simulations COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/09/10 CRDT- 2026/10/28 05:39 PHST- 2026/02/24 00:00 [received] PHST- 2026/08/02 00:00 [revised] PHST- 2026/09/07 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:39 [entrez] PHST- 2026/09/10 00:00 [pmc-release] AID - S1226-8453(26)00178-8 [pii] AID - 101152 [pii] AID - 10.1016/j.jgr.2026.101152 [doi] PST - ppublish SO - J Ginseng Res. 2026 Nov;50(6):101152. doi: 10.1016/j.jgr.2026.101152. Epub 2026 Sep 10. PMID- 42948574 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2666-2736 (Electronic) IS - 2666-2736 (Linking) VI - 33 DP - 2026 Oct TI - Is pneumonectomy appropriate after chemoimmunotherapy? Outcomes of neoadjuvant chemoimmunotherapy followed by pneumonectomy in the United States. PG - 102047 LID - 10.1016/j.xjon.2026.102047 [doi] LID - 102047 AB - OBJECTIVE: The appropriateness of pneumonectomy for non-small cell lung cancer after neoadjuvant therapy is not well characterized, and there is concern for substantially greater mortality on the basis of results of trials such as Intergroup-0139. Here, we present current use and outcomes of pneumonectomy after neoadjuvant chemoimmunotherapy. METHODS: Using the National Cancer Database, we identified and included adults with clinical stage I-III lung adenocarcinoma or squamous cell carcinoma who were diagnosed between 2018 and 2023 and treated with neoadjuvant chemoimmunotherapy followed by lobectomy or pneumonectomy. Three-year overall survival was assessed by Kaplan-Meier. Propensity matching and Cox proportional hazards models were based on patient and tumor characteristics. RESULTS: The study included 2703 (94%) lobectomies and 173 (6%) pneumonectomies. Patients who underwent pneumonectomy had a more advanced T stage (64% vs 47% T3+, P < .001) but a lower comorbidity burden (71% vs 58% Charlson-Deyo of 0, P = .01). They had greater rates of 30-day unplanned readmission (9% vs 4%, P = .001) and R1+ resection (7.1% vs 3.9%, P = .046) but comparable 90-day mortality (<4.5% vs 2%, P = .24). Unadjusted 3-year overall survival was not significantly different between the 2 groups: 80% for pneumonectomy and 82% for lobectomy (P = .08). Similar trends were observed after propensity-matching for patient and tumor characteristics: 79% for pneumonectomy and 78% for lobectomy (P = .19). On Cox models, pneumonectomy was associated with a greater hazard ratio of mortality, but this was not statistically significant (odds ratio, 1.56; 95% CI, 0.91-2.68; P = .09). CONCLUSIONS: Pneumonectomy after induction chemoimmunotherapy demonstrated comparable early survival with lobectomy among selected patients in the United States. Use of pneumonectomy after chemoimmunotherapy in appropriate candidates appears reasonable. CI - (c) 2026 The Author(s). FAU - Caturegli, Giorgio AU - Caturegli G AD - Division of Thoracic Surgery, Department of Surgery, Yale School of Medicine, New Haven, Conn. FAU - Jernigan, Ebony AU - Jernigan E AD - Division of Thoracic Surgery, Department of Surgery, Yale School of Medicine, New Haven, Conn. FAU - Canavan, Maureen AU - Canavan M AD - Division of Thoracic Surgery, Department of Surgery, Yale School of Medicine, New Haven, Conn. AD - Yale Cancer Outcomes, Public Policy and Effectiveness Research (COPPER) Center, Yale School of Medicine, New Haven, Conn. FAU - Lynch, Abigail AU - Lynch A AD - Division of Thoracic Surgery, Department of Surgery, Yale School of Medicine, New Haven, Conn. FAU - Kramer, Ryan AU - Kramer R AD - Division of Thoracic Surgery, Department of Surgery, Yale School of Medicine, New Haven, Conn. FAU - Patil, Jayaditya AU - Patil J AD - Division of Thoracic Surgery, Department of Surgery, Yale School of Medicine, New Haven, Conn. FAU - Esnaola, Gabriela AU - Esnaola G AD - Division of Thoracic Surgery, Department of Surgery, Yale School of Medicine, New Haven, Conn. FAU - Boffa, Daniel J AU - Boffa DJ AD - Division of Thoracic Surgery, Department of Surgery, Yale School of Medicine, New Haven, Conn. FAU - Resio, Benjamin J AU - Resio BJ AD - Division of Thoracic Surgery, Department of Surgery, Yale School of Medicine, New Haven, Conn. LA - eng PT - Journal Article DEP - 20260811 PL - Netherlands TA - JTCVS Open JT - JTCVS open JID - 101768541 PMC - PMC13711314 OTO - NOTNLM OT - chemoimmunotherapy OT - immunotherapy OT - lung cancer OT - pneumonectomy OT - surgery COIS- The authors reported no conflicts of interest. The Journal policy requires editors and reviewers to disclose conflicts of interest and to decline handling or reviewing manuscripts for which they may have a conflict of interest. The editors and reviewers of this article have no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/08/11 CRDT- 2026/10/28 05:39 PHST- 2026/04/25 00:00 [received] PHST- 2026/07/03 00:00 [revised] PHST- 2026/07/18 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:39 [entrez] PHST- 2026/08/11 00:00 [pmc-release] AID - S2666-2736(26)00470-5 [pii] AID - 102047 [pii] AID - 10.1016/j.xjon.2026.102047 [doi] PST - epublish SO - JTCVS Open. 2026 Aug 11;33:102047. doi: 10.1016/j.xjon.2026.102047. eCollection 2026 Oct. PMID- 42948570 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2398-8835 (Electronic) IS - 2398-8835 (Linking) VI - 9 IP - 11 DP - 2026 Nov TI - Hepatitis C Micro-Elimination Through Patient Navigation of Individuals Experiencing Elevated Risk in a Mid-Atlantic Regional Healthcare System in 2022 and 2023: A Cohort Study. PG - e73447 LID - 10.1002/hsr2.73447 [doi] LID - e73447 AB - BACKGROUND AND AIMS: Hepatitis C virus (HCV) disproportionately impacts persons with substance use and mental health disorders. Micro-elimination projects selectively foster diagnosis, treatment and reinfection of high priority demographic groups. A small-scale, peer navigation focused micro-elimination project was launched at a regional multidisciplinary and multispecialty health system in the Mid-Atlantic to facilitate treatment engagement for high-risk individuals. METHODS: The program used electronic medical record (EMR) periodic surveillance and navigation of individuals diagnosed with HCV and either not engaged, or not completed, treatment, as well as those needing additional testing (for either sustained virologic response or high-risk re-testing). Specialist appointments and referrals for associated behavioral health interventions were provided. Historical propensity score matched controls were utilized to provide a comparator population. RESULTS: Between April 2022 and July 2023, 115 persons were navigated, including 78 persons without a prior specialist appointment (New Patients), 15 persons previously engaged with a specialist but not completing treatment, and 22 patients needing additional testing. Among New Patients, 52.6% of patients attended treatment appointments, 28.2% started treatment, and 14.1% had a confirmed undetectable HCV test. Compared to historical matched controls, persons navigated were almost twice as likely to attend an appointment (HR: 1.96 [1.18-3.23], p = 0.008), and nearly three times more likely to start treatment (HR: 2.86 [1.41-5.79], p = 0.003). The median time from navigation to an appointment for New Patients was 71 days, and 143 days until starting treatment. CONCLUSIONS: A small-scale, peer-navigation focused micro-navigation program successfully engaged patients but had mixed success in successfully navigating the care cascade. Despite navigation and consistent engagement, nearly half of patients dropped off at each step of the care cascade. Compared to historical controls, patients were more likely to make appointments and initiate treatment. Future efforts should be targeted at reducing times for specialist appointments and expanding treatment at diagnosis location. CI - (c) 2026 The Author(s). Health Science Reports published by Wiley Periodicals LLC. FAU - Visconti, Adam AU - Visconti A AUID- ORCID: 0000-0001-6257-1691 AD - Department of Family Medicine MedStar Georgetown University Washington District of Columbia USA. AD - Georgetown University School of Medicine Washington District of Columbia USA. FAU - Garner, Demetrie AU - Garner D AD - MedStar Health Research Institute Hyattsville Maryland USA. FAU - Gabra, Jessica AU - Gabra J AD - Department of Family Medicine MedStar Georgetown University Washington District of Columbia USA. AD - Georgetown University School of Medicine Washington District of Columbia USA. FAU - Fernandez, Stephen AU - Fernandez S AUID- ORCID: 0000-0002-5112-1844 AD - MedStar Health Research Institute Hyattsville Maryland USA. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Health Sci Rep JT - Health science reports JID - 101728855 PMC - PMC13711362 OTO - NOTNLM OT - hepatitis C OT - micro-elimination OT - patient navigation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/27 CRDT- 2026/10/28 05:39 PHST- 2025/09/29 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:39 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - HSR273447 [pii] AID - 10.1002/hsr2.73447 [doi] PST - epublish SO - Health Sci Rep. 2026 Oct 27;9(11):e73447. doi: 10.1002/hsr2.73447. eCollection 2026 Nov. PMID- 42948567 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2666-2736 (Electronic) IS - 2666-2736 (Linking) VI - 33 DP - 2026 Oct TI - Endoscopic versus open vein harvesting technique and 2-year saphenous vein graft patency: A subanalysis of the Newton-CABG CardioLink-5 randomized trial. PG - 102063 LID - 10.1016/j.xjon.2026.102063 [doi] LID - 102063 FAU - Verma, Raj AU - Verma R AD - Royal College of Surgeons in Ireland, Dublin, Ireland. FAU - Teoh, Hwee AU - Teoh H AD - Division of Cardiac Surgery, St Michael's Hospital-Unity Health Toronto, Toronto, Ontario, Canada. AD - Division of Endocrinology and Metabolism, St Michael's Hospital-Unity Health Toronto, Toronto, Ontario, Canada. AD - Li Ka Shing Knowledge Institute, St Michael's Hospital-Unity Health Toronto, Toronto, Ontario, Canada. FAU - Leiter, Lawrence A AU - Leiter LA AD - Division of Endocrinology and Metabolism, St Michael's Hospital-Unity Health Toronto, Toronto, Ontario, Canada. AD - Li Ka Shing Knowledge Institute, St Michael's Hospital-Unity Health Toronto, Toronto, Ontario, Canada. AD - Department of Medicine, University of Toronto, Toronto, Ontario, Canada. AD - Department of Nutritional Sciences, University of Toronto, Toronto, Ontario, Canada. AD - Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada. FAU - Mancini, G B John AU - Mancini GBJ AD - Division of Cardiology, Centre for Cardiovascular Innovation, Dilawri Cardiovascular Institute, University of British Columbia, Vancouver, British Columbia, Canada. FAU - Quan, Adrian AU - Quan A AD - Division of Cardiac Surgery, St Michael's Hospital-Unity Health Toronto, Toronto, Ontario, Canada. AD - Li Ka Shing Knowledge Institute, St Michael's Hospital-Unity Health Toronto, Toronto, Ontario, Canada. FAU - Elituv, Randi AU - Elituv R AD - Division of Cardiac Surgery, St Michael's Hospital-Unity Health Toronto, Toronto, Ontario, Canada. AD - North York Diagnostic and Cardiac Centre, Toronto, Ontario, Canada. FAU - Verma, Meena AU - Verma M AD - Royal College of Surgeons in Ireland, Dublin, Ireland. FAU - Patel, Shubh K AU - Patel SK AD - Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada. FAU - Szarek, Michael AU - Szarek M AD - Division of Cardiology, University of Colorado School of Medicine, Aurora, Colo. AD - CPC Clinical Research, University of Colorado School of Medicine, Aurora, Colo. AD - Department of Epidemiology and Biostatistics, State University of New York, Downstate Health Sciences University, Brooklyn, NY. AD - Mount Sinai Fuster Heart Hospital, Icahn School of Medicine at Mount Sinai, New York, NY. FAU - Juni, Peter AU - Juni P AD - Institute of Health Policy, Management, and Evaluation, University of Toronto, Toronto, Ontario, Canada. AD - Clinical Trial Service Unit and Epidemiological Studies Unit (CTSU), Nuffield Department of Population Health, University of Oxford, Oxford, Oxfordshire, United Kingdom. FAU - Koren, Michael J AU - Koren MJ AD - Jacksonville Center for Clinical Research, Flourish Research Group, Jacksonville, Fla. FAU - Nicholls, Stephen J AU - Nicholls SJ AD - Victorian Heart Institute, Monash University, Melbourne, Victoria, Australia. FAU - Verma, Subodh AU - Verma S AD - Division of Cardiac Surgery, St Michael's Hospital-Unity Health Toronto, Toronto, Ontario, Canada. AD - Li Ka Shing Knowledge Institute, St Michael's Hospital-Unity Health Toronto, Toronto, Ontario, Canada. AD - Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada. AD - North York Diagnostic and Cardiac Centre, Toronto, Ontario, Canada. AD - Department of Surgery, University of Toronto, Toronto, Ontario, Canada. AD - Department of Pharmacology and Toxicology, University of Toronto, Toronto, Ontario, Canada. FAU - Bhatt, Deepak L AU - Bhatt DL AD - Mount Sinai Fuster Heart Hospital, Icahn School of Medicine at Mount Sinai, New York, NY. FAU - Mazer, C David AU - Mazer CD AD - Li Ka Shing Knowledge Institute, St Michael's Hospital-Unity Health Toronto, Toronto, Ontario, Canada. AD - Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada. AD - Department of Pharmacology and Toxicology, University of Toronto, Toronto, Ontario, Canada. AD - Department of Anesthesia, St Michael's Hospital-Unity Health Toronto, Toronto, Ontario, Canada. AD - Department of Anesthesiology and Pain Medicine, University of Toronto, Toronto, Ontario, Canada. AD - Department of Physiology, University of Toronto, Toronto, Ontario, Canada. LA - eng PT - Journal Article DEP - 20260818 PL - Netherlands TA - JTCVS Open JT - JTCVS open JID - 101768541 PMC - PMC13711310 COIS- S.V. holds a Tier 1 Canada Research Chair in Cardiovascular Surgery. C.D.M. is supported by a Merit Award from the University of Toronto Department of Anesthesiology and Pain Medicine and holds the Cara Phelan Chair in Critical Care at St Michael's Hospital-Unity Health Toronto. H.T. reports personal fees from the Canadian Medical and Surgical Knowledge Translation Research Group and LMC Healthcare. L.A.L. reports research support from, speaking honoraria, and/or acting as an advisor to Abbott, Amgen, AstraZeneca, Boehringer Ingelheim, Eli Lilly, GSK, HLS Therapeutics, Merck, Novartis, Novo Nordisk, and Regeneron as well as a member of a data safety monitoring board for CRISPR Therapeutics and vTv Therapeutics. G.B.J.M. reports research support from, speaking honoraria, and/or acting as an advisor to Amgen, AstraZeneca, Boehringer Ingelheim, Eli Lilly, GSK, HLS Therapeutics, Merck, Novartis, Novo Nordisk, Esperion, and Ultragenyx. M.S. reports salary support from CPC, a non-profit academic research organization affiliated with the University of Colorado, that receives or has received research grant/consulting funding between July 2021 and July 2025 from the following organizations: 35Pharma, Inc, Abbott Laboratories, Agios Pharmaceuticals, Inc, Alexion Pharma Godo Kaisha, the American Heart Association, the American Journal of Managed Care, Amgen Inc, Amgen USA, Inc, Anthos Therapeutics, Inc, Arrowhead Pharmaceuticals, AstraZeneca Pharma India, AstraZeneca Pharmaceuticals LP, AstraZeneca UK Ltd, Autonomy Bio, Inc, Bayer, Bayer Aktiengesellschaft, Beth Israel Deaconess Medical Center, Better Therapeutics, Boston Clinical Research Institute, LLC, Bristol-Myers Squibb, Cleerly, Inc, Clergy United for the Transformation of Sandtown, Colorado Department of Public Health and Environment, Congress Inc, Cook Regentec LLC, Eidos Therapeutics, Inc., EluraBio, Inc, Esperion Therapeutics, Inc, Faraday Pharmaceuticals, Inc, Gasherbrum Bio Inc, Insmed, IsomAb Limited, JanOne Biotech Holdings, Inc, Janssen Global Services, Janssen Pharmaceuticals, Inc, Janssen Scientific Affairs LLC, Las Animas and Huerfano Counties, District Health Dept, Lexicon Pharmaceuticals, Inc., Lilly USA, LLC, Medison Pharma, Medpace, Inc, Merck Sharp & Dohme Corp, Nectero Medical, Inc, NewAmsterdam Pharma, Novartis Pharmaceuticals Corporation, Novo Nordisk Inc, Pfizer, Piper Sandler & Co, PPD Development, LP, Prothena Biosciences Limited, Regeneron, Regents of the University of Colorado (aka UCD), Sanifit Therapeutics S.A., Sanofi, Silence Therapeutics PLC, Stanford University, Stealth BioTherapeutics Inc, The Brigham and Women's Hospital, Thrombosis Research Institute, Tourmaline Bio, Inc, University of Colorado, University of Pittsburgh, VarmX, Verve Therapeutics, and WraSer, LLC, and consultant or research support (or both) from Amarin, Lexicon, NewAmsterdam Pharma, Novartis, Regeneron, Sanofi, Silence, and Tourmaline. M.J.K. is an employee of a clinical research company supported by grants from multiple manufacturers of lipid and other therapeutics. S.J.N. reports grant/research support from Amgen, Anthera, AstraZeneca, Cerenis, Cyclarity, Eli Lilly, Esperion, InfraReDx, NewAmsterdam Pharma, Novartis, Resverlogix, Roche, Sanofi-Regeneron, The Medicines Company, and LipoScience and was a consultant for Abcentra, Akcea, Amarin, Anthera, AstraZeneca, Boehringer Ingelheim, CSL Behring, CSL Seqirus, Daiichi Sankyo, Eli Lilly, Esperion, Merck, Omthera, Resverlogix, Sanofi-Regeneron, Scribe, Silence Therapeutics, Takeda, and Vaxxinity. S.V. reports grants and/or research support and/or speaking honoraria from Amgen, AstraZeneca, Bayer, Boehringer Ingelheim, Canadian Heart Research Centre, Canadian Medical and Surgical Knowledge Translation Research Group, Eli Lilly, HLS Therapeutics, Humber River Health, Janssen, Merck, Novartis, Novo Nordisk, Pfizer, PhaseBio, S & L Solutions Event Management Inc, Sanofi, and Sun Pharma. D.L.B. reports advisory board: Angiowave, Antlia Bioscience, Bayer, Boehringer Ingelheim, CellProthera, Cereno Scientific, E-Star Biotech, High Enroll, Janssen, Level Ex, McKinsey, Medscape Cardiology, Merck, NirvaMed, Novo Nordisk, Repair Biotechnologies, Stasys, and Tourmaline Bio; board of directors: the American Heart Association New York City, Angiowave (stock options), Bristol Myers Squibb (stock), DRS.LINQ (stock options), and High Enroll (stock); consultant: Alnylam, Altimmune, Broadview Ventures, Corcept Therapeutics, Corsera, GlaxoSmithKline, Hims, SERB, SFJ, Summa Therapeutics, and Worldwide Clinical Trials; data monitoring committees: Acesion Pharma, Assistance Publique-Hopitaux de Paris, Baim Institute for Clinical Research, Boston Scientific (Chair, PEITHO trial), Cleveland Clinic, Contego Medical (Chair, PERFORMANCE 2), Duke Clinical Research Institute, Mayo Clinic, Mount Sinai School of Medicine (for the ABILITY-DM trial, funded by Concept Medical; for ALLAY-HF, funded by Alleviant Medical), Novartis, and Population Health Research Institute; Rutgers University (for the National Institutes of Health-funded MINT Trial); honoraria: American College of Cardiology (Senior Associate Editor, Clinical Trials and News, ACC.org; Chair, ACC Accreditation Oversight Committee), Arnold and Porter law firm (work related to Sanofi/Bristol-Myers Squibb clopidogrel litigation), Baim Institute for Clinical Research (AEGIS-II executive committee funded by CSL Behring), Belvoir Publications (Editor in Chief, Harvard Heart Letter), Canadian Medical and Surgical Knowledge Translation Research Group (clinical trial steering committees), CSL Behring (AHA lecture), Duke Clinical Research Institute, Engage Health Media, HMP Global (Editor in Chief, Journal of Invasive Cardiology), Medtelligence/ReachMD (CME steering committees), MJH Life Sciences, Oakstone CME (Course Director, Comprehensive Review of Interventional Cardiology), Philips (Becker's Webinar on AI), Population Health Research Institute, WebMD (CME steering committees), and Wiley (steering committee); and other: Clinical Cardiology (Deputy Editor); Progress in Cardiovascular Diseases (Deputy Editor); patent: Sotagliflozin (named on a patent for sotagliflozin assigned to Brigham and Women's Hospital who assigned to Lexicon; neither S.V. nor Brigham and Women's Hospital receive any income from this patent); research funding: Abbott, Acesion Pharma, Afimmune, Alnylam, Amarin, Amgen, AstraZeneca, Atricure, Bayer, Boehringer Ingelheim, Boston Scientific, CellProthera, Cereno Scientific, Chiesi, Cleerly, CSL Behring, Faraday Pharmaceuticals, Fractyl, Idorsia, Janssen, Javelin, Lexicon, Lilly, Medtronic, Merck, MiRUS, Moderna, Novartis, Novo Nordisk, Pfizer, PhaseBio, Regeneron, Reid Hoffman Foundation, Roche, Sanofi, Stasys, and 89Bio; royalties: Elsevier (Editor, Braunwald's Heart Disease); and Site Co-Investigator: Cleerly. C.D.M. received advisory board honoraria/consulting fees from Amgen, Alexion, AstraZeneca, BioAge, Biotest, Boehringer Ingelheim, Cardior, CytoSorbents, Delex, ONA, PhaseBio, Sandoz, Trimedic Therapeutics, VarmX, and Werfen as well as data safety monitoring board stipends from Beth Israel Deaconess Medical Center, Cerus, and Takeda. All other authors reported no conflicts of interest. The Journal policy requires editors and reviewers to disclose conflicts of interest and to decline handling or reviewing manuscripts for which they may have a conflict of interest. The editors and reviewers of this article have no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/08/18 CRDT- 2026/10/28 05:39 PHST- 2026/05/02 00:00 [received] PHST- 2026/07/03 00:00 [revised] PHST- 2026/07/23 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:39 [entrez] PHST- 2026/08/18 00:00 [pmc-release] AID - S2666-2736(26)00486-9 [pii] AID - 102063 [pii] AID - 10.1016/j.xjon.2026.102063 [doi] PST - epublish SO - JTCVS Open. 2026 Aug 18;33:102063. doi: 10.1016/j.xjon.2026.102063. eCollection 2026 Oct. PMID- 42948564 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2688-2663 (Electronic) IS - 2688-2663 (Linking) VI - 7 IP - 11 DP - 2026 Nov TI - Liquid Biopsy: Past, Present, and Future. PG - e71085 LID - 10.1002/mco2.71085 [doi] LID - e71085 AB - Liquid biopsy has evolved over two decades from an extension of tissue genotyping into a minimally invasive approach for repeat and longitudinal molecular assessment across the cancer continuum. Advances in sequencing, digital PCR, error suppression, and computational analytics have expanded analytes beyond circulating tumor DNA and circulating tumor cells to extracellular vesicles, circulating RNAs, and tumor-educated platelets, while broadening readouts from mutations to methylation, fragmentomics, transcriptomics, proteomics, and multiomic integration. This review traces the field from its technological foundations to current applications in early detection and multicancer early detection, therapy selection and resistance monitoring, minimal residual disease, recurrence-risk stratification, and longitudinal response assessment across major cancers and selected nononcologic settings. We distinguish clinically established applications from emerging and exploratory strategies and emphasize that high analytical performance does not by itself establish clinical utility. We further discuss preanalytical handling, standardization, biological confounders, and evidence required for implementation. Future progress will depend on use-case-driven development, prospective utility studies, longitudinal and multimodal interpretation, and integration with established diagnostic pathways. Together, these priorities define a transition from molecular detectability toward reproducible, actionable, and clinically meaningful liquid biopsy. CI - (c) 2026 The Author(s). MedComm published by Sichuan International Medical Exchange & Promotion Association (SCIMEA) and John Wiley & Sons Australia, Ltd. FAU - Liu, Jiachen AU - Liu J AD - Department of Urology The Second Xiangya Hospital at Central South University Changsha Hunan China. AD - Xiangya School of Medicine Central South University Changsha Hunan China. AD - Department of Urology Xiangya Hospital Central South University Changsha Hunan China. AD - Washington University School of Medicine St Louis Missouri USA. FAU - Zeng, Dianjie AU - Zeng D AUID- ORCID: 0009-0006-4803-0833 AD - Department of Urology The Second Xiangya Hospital at Central South University Changsha Hunan China. AD - Xiangya School of Medicine Central South University Changsha Hunan China. FAU - Zhao, Keran AU - Zhao K AD - Xiangya School of Medicine Central South University Changsha Hunan China. FAU - Wang, Yuxi AU - Wang Y AD - Xiangya School of Medicine Central South University Changsha Hunan China. FAU - Hu, Huijie AU - Hu H AD - Guangdong Medical University Dongguan Guangdong China. FAU - Deng, Fei AU - Deng F AD - Department of Urology The Second Xiangya Hospital at Central South University Changsha Hunan China. FAU - Wang, Yinhuai AU - Wang Y AD - Department of Urology The Second Xiangya Hospital at Central South University Changsha Hunan China. FAU - Sun, Liya AU - Sun L AD - Department of Nephrology Xiangya Hospital at Central South University Changsha Hunan China. FAU - Fu, Liangmin AU - Fu L AD - Department of Urology The Second Xiangya Hospital at Central South University Changsha Hunan China. AD - National Clinical Research Center for Metabolic Disease Key Laboratory of Diabetes Immunology (Central South University) Ministry of Education Changsha Hunan China. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - China TA - MedComm (2020) JT - MedComm JID - 101769925 PMC - PMC13711345 OTO - NOTNLM OT - circulating tumor DNA (ctDNA) OT - circulating tumor cells (CTCs) OT - extracellular vesicles (EVs) OT - liquid biopsy OT - minimal residual disease (MRD) OT - multicancer early detection (MCED) COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/27 CRDT- 2026/10/28 05:39 PHST- 2025/01/25 00:00 [received] PHST- 2026/09/02 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:39 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - MCO271085 [pii] AID - 10.1002/mco2.71085 [doi] PST - epublish SO - MedComm (2020). 2026 Oct 27;7(11):e71085. doi: 10.1002/mco2.71085. eCollection 2026 Nov. PMID- 42948561 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2397-9070 (Electronic) IS - 2397-9070 (Linking) VI - 10 IP - 10 DP - 2026 Oct TI - von Willebrand Factor and ADAMTS13 Predict Therapeutic Efficacy and Severe Adverse Events in Advanced Hepatocellular Carcinoma Treated With Atezolizumab-Bevacizumab. PG - e70484 LID - 10.1002/jgh3.70484 [doi] LID - e70484 AB - AIMS: Atezolizumab combined with bevacizumab (AB therapy) is a standard first-line treatment for advanced hepatocellular carcinoma (HCC). However, clinically useful biomarkers for predicting therapeutic efficacy and severe adverse events (AEs) remain limited. Beyond its role in thrombosis, ADAMTS13-von Willebrand factor (VWF) axis is increasingly recognized for its involvement in angiogenesis and immune modulation. This study aimed to investigate the clinical significance of the ADAMTS13-VWF axis in patients receiving AB therapy. METHODS: A total of 58 patients with advanced HCC treated with AB therapy were enrolled. Plasma VWF antigen (VWF:Ag) and ADAMTS13 activity (ADAMTS13:AC) were measured before treatment initiation. Therapeutic efficacy was assessed using Response Evaluation Criteria in Solid Tumors (RECIST) version 1.1 and modified RECIST (mRECIST), while safety was assessed according to Common Terminology Criteria for AEs version 5.0. RESULTS: Multivariate analyses identified lower baseline VWF:Ag (< 180%) and ADAMTS13:AC (/= 3). Plasma VWF:Ag and ADAMTS13:AC levels did not significantly change after AB therapy. CONCLUSION: Baseline VWF:Ag and ADAMTS13:AC independently predict therapeutic efficacy, whereas the baseline VWF/ADAMTS13 ratio independently predicts severe AEs in patients with HCC receiving AB therapy. These biomarkers may help optimize patient selection and manage treatment-related toxicities by reflecting tumor angiogenic activity and host immune status. CI - (c) 2026 The Author(s). JGH Open published by Journal of Gastroenterology and Hepatology Foundation and John Wiley & Sons Australia, Ltd. FAU - Nishimura, Naoki AU - Nishimura N AD - Department of Gastroenterology Nara Medical University Nara Japan. FAU - Takaya, Hiroaki AU - Takaya H AUID- ORCID: 0000-0002-4990-7573 AD - Department of Gastroenterology Nara Medical University Nara Japan. FAU - Namisaki, Tadashi AU - Namisaki T AUID- ORCID: 0000-0002-3158-5318 AD - Department of Gastroenterology Nara Medical University Nara Japan. FAU - Komeda, Yusuke AU - Komeda Y AD - Department of Gastroenterology Nara Medical University Nara Japan. FAU - Iwai, Satoshi AU - Iwai S AUID- ORCID: 0000-0003-3575-5609 AD - Department of Gastroenterology Nara Medical University Nara Japan. FAU - Shibamoto, Akihiko AU - Shibamoto A AUID- ORCID: 0009-0002-3665-0454 AD - Department of Gastroenterology Nara Medical University Nara Japan. FAU - Tsuji, Yuki AU - Tsuji Y AD - Department of Gastroenterology Nara Medical University Nara Japan. FAU - Fujinaga, Yukihisa AU - Fujinaga Y AUID- ORCID: 0000-0003-2402-574X AD - Department of Gastroenterology Nara Medical University Nara Japan. FAU - Kitagawa, Koh AU - Kitagawa K AUID- ORCID: 0000-0001-5794-1512 AD - Department of Gastroenterology Nara Medical University Nara Japan. FAU - Sato, Shinya AU - Sato S AUID- ORCID: 0000-0003-3049-3443 AD - Department of Gastroenterology Nara Medical University Nara Japan. FAU - Nishimura, Norihisa AU - Nishimura N AUID- ORCID: 0000-0002-6295-3283 AD - Department of Gastroenterology Nara Medical University Nara Japan. FAU - Kaji, Kosuke AU - Kaji K AUID- ORCID: 0000-0002-1822-6759 AD - Department of Gastroenterology Nara Medical University Nara Japan. FAU - Yoshiji, Hitoshi AU - Yoshiji H AUID- ORCID: 0000-0002-5243-8544 AD - Department of Gastroenterology Nara Medical University Nara Japan. LA - eng PT - Journal Article DEP - 20261027 PL - Australia TA - JGH Open JT - JGH open : an open access journal of gastroenterology and hepatology JID - 101730833 PMC - PMC13711347 OTO - NOTNLM OT - angiogenesis OT - bevacizumab OT - biomarkers OT - liver neoplasms OT - thrombosis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/27 CRDT- 2026/10/28 05:38 PHST- 2026/06/30 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:38 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - JGH370484 [pii] AID - 10.1002/jgh3.70484 [doi] PST - epublish SO - JGH Open. 2026 Oct 27;10(10):e70484. doi: 10.1002/jgh3.70484. eCollection 2026 Oct. PMID- 42948552 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2673-4915 (Electronic) IS - 2673-4915 (Linking) VI - 7 DP - 2026 TI - Salivary extracellular vesicles in precision periodontology: diagnostic biomarkers and therapeutic opportunities. PG - 1964471 LID - 10.3389/fdmed.2026.1964471 [doi] LID - 1964471 AB - Periodontitis is a chronic inflammatory disease in which clinical and radiographic assessments reflect the consequences of tissue destruction rather than ongoing biological activity. Salivary Extracellular Vesicles (EVs) have emerged as a promising source of minimally invasive biomarkers capable of reflecting real-time periodontal biology, owing to their stable, membrane-protected cargo of proteins, lipids, and regulatory nucleic acids. Current studies suggest that EVs-associated microRNAs and proteins may help distinguish periodontal health from disease and may reflect biological changes following periodontal therapy. This mini review discusses the biological origin and cargo of salivary EVs and examines their emerging relevance to precision periodontology. EVs derived from mesenchymal and periodontal ligament stem cells also show immunomodulatory, osteogenic, and regenerative effects in experimental models. Although diagnostic and therapeutic evidence is still emerging, growing findings support the potential of EVs-based approaches in periodontology. Further progress through standardised EVs isolation and characterisation, robust biomarker validation, and well-designed clinical studies will facilitate their translation into personalised periodontal care. CI - (c) 2026 Vineela, Rao and Mahadevan. FAU - Vineela, Amanchi AU - Vineela A AD - Department of Periodontology, AB Shetty Memorial Institute of Dental Sciences (ABSMIDS), Nitte (Deemed to be University), Mangalore, India. FAU - Rao, Amita AU - Rao A AD - Department of Periodontology, AB Shetty Memorial Institute of Dental Sciences (ABSMIDS), Nitte (Deemed to be University), Mangalore, India. FAU - Mahadevan, Srilakshmi AU - Mahadevan S AD - Department of Periodontology, AB Shetty Memorial Institute of Dental Sciences (ABSMIDS), Nitte (Deemed to be University), Mangalore, India. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Dent Med JT - Frontiers in dental medicine JID - 101777956 PMC - PMC13711376 OTO - NOTNLM OT - biomarkers OT - extracellular vesicles OT - periodontitis OT - precision periodontology OT - saliva COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:38 PHST- 2026/08/11 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:38 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fdmed.2026.1964471 [doi] PST - epublish SO - Front Dent Med. 2026 Oct 13;7:1964471. doi: 10.3389/fdmed.2026.1964471. eCollection 2026. PMID- 42948533 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2005-7806 (Print) IS - 2005-7814 (Electronic) IS - 2005-7806 (Linking) VI - 18 IP - 5 DP - 2026 Oct TI - Prosthodontic planning for adults with functional limitations: a feasibility and maintenance framework. PG - 520-532 LID - 10.4047/jap.2026.18.5.520 [doi] AB - Some patients with cognitive, developmental, neurologic, or physical conditions require modified prosthodontic care. However, they often receive less restorative and prosthodontic treatment than needed. This narrative review explores how conventional prosthodontic treatment planning should be complemented when treatment delivery and long-term maintenance depend on functional capacity and caregiver support. The available treatment options horizontal line including restorations of natural teeth, tooth-supported fixed dental prostheses, removable dental prostheses, implant-assisted removable prostheses, implant-supported fixed dental prostheses, and limited or deferred treatment horizontal line are broadly similar to those offered to the general population. However, treatment selection should additionally consider whether the proposed procedures can be completed, whether the restoration or prosthesis can be used safely and effectively, and whether long-term maintenance can be sustained by the patient, caregivers, and available support services. Treatment decisions should balance the anticipated benefits against the treatment burden while respecting patient preferences, decision-making capacity, informed consent or assent, and the potential need for definitive treatment under sedation or general anesthesia. Current evidence is largely retrospective, indirect, and derived from a limited number of centers. Accordingly, this review proposes a feasibility- and maintenance-oriented framework to support clinical decision-making, complementing, rather than replacing, conventional anatomical and biomechanical treatment planning. CI - (c) 2026 The Korean Academy of Prosthodontics. FAU - Yoo, Soo-Yeon AU - Yoo SY AUID- ORCID: 0000-0002-5171-6426 AD - Department of Prosthodontics & Dental Research Institute, Seoul National University Dental Hospital, School of Dentistry, Seoul National University, Seoul, Republic of Korea. FAU - Diz Dios, Pedro AU - Diz Dios P AUID- ORCID: 0000-0002-1483-401X AD - Medical-Surgical Dentistry Research Group (OMEQUI), Health Research Institute of Santiago de Compostela (IDIS), University of Santiago de Compostela (USC), Santiago de Compostela, Spain. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Korea (South) TA - J Adv Prosthodont JT - The journal of advanced prosthodontics JID - 101521064 PMC - PMC13713204 OTO - NOTNLM OT - Dental care for disabled OT - Dental prosthesis OT - Disabled persons OT - Patient care planning OT - Prosthodontics EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/01 CRDT- 2026/10/28 05:37 PHST- 2026/08/06 00:00 [received] PHST- 2026/08/20 00:00 [revised] PHST- 2026/08/24 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:37 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - 10.4047/jap.2026.18.5.520 [doi] PST - ppublish SO - J Adv Prosthodont. 2026 Oct;18(5):520-532. doi: 10.4047/jap.2026.18.5.520. Epub 2026 Oct 13. PMID- 42948523 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-8021 (Print) IS - 1664-8021 (Electronic) IS - 1664-8021 (Linking) VI - 17 DP - 2026 TI - The immunogenicity control axis: a multi-omics framework for integrating epigenetic, DNA damage response, and immune states in cancer. PG - 1944709 LID - 10.3389/fgene.2026.1944709 [doi] LID - 1944709 AB - Immunotherapy has transformed cancer treatment, yet therapeutic responses remain heterogeneous and are incompletely explained by immune-centric biomarkers alone. We propose the Immunogenicity Control Axis (ICA), a testable multi-omics framework integrating three complementary dimensions of tumor state: epigenetic regulation, DNA damage response (DDR), and immune activity. The central hypothesis is that epigenetic permissiveness influences immune-relevant transcription and antigen presentation, while DDR-associated genomic processes contribute to antigenic variation; together, these tumor-intrinsic features interact with the immune state to shape tumor immune visibility. To make this hypothesis quantitatively testable, the ICA is operationalized through independently derived Epigenetic, DDR, and Immune scores that are standardized and integrated into a composite ICA Score. Using cervical cancer as an initial disease context, patient-derived TCGA-CESC analyses provide exploratory assessment of ICA-associated molecular states and prognostic relationships, while synthetic analyses are used separately to illustrate specific analytical procedures. The framework is further extended to perturbational analysis, in which CMap/LINCS transcriptional signatures and network information are used to prioritize candidate natural compounds predicted to oppose ICA-associated molecular states. These computational analyses are intended as hypothesis-generating operationalizations rather than biological or clinical validation. ICA therefore provides a falsifiable framework for testing whether coordinated epigenetic, DDR, and immune information captures tumor immunogenic variation beyond individual molecular dimensions and for prioritizing perturbational hypotheses for subsequent experimental validation. CI - Copyright (c) 2026 Srivastava, Singh, Ameta, Singh, Banerjee and Kushwah. FAU - Srivastava, Manyata AU - Srivastava M AD - Department of Bioscience, Manipal University Jaipur, Jaipur, Rajasthan, India. FAU - Singh, Shishir AU - Singh S AD - Division of Immunobiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, United States. FAU - Ameta, Diksha AU - Ameta D AD - Department of Bioscience, Manipal University Jaipur, Jaipur, Rajasthan, India. FAU - Singh, Aesha AU - Singh A AD - Department of Internal Medicine, Marshall University, Huntington, WV, United States. FAU - Banerjee, Monisha AU - Banerjee M AD - Department of Zoology, University of Lucknow, Lucknow, India. FAU - Kushwah, Atar Singh AU - Kushwah AS AD - Department of Bioscience, Manipal University Jaipur, Jaipur, Rajasthan, India. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Front Genet JT - Frontiers in genetics JID - 101560621 PMC - PMC13713020 OTO - NOTNLM OT - DNA damage response OT - cervical cancer OT - epigenetics OT - immunogenicity control axis OT - immunotherapy resistance OT - multi-omics integration OT - natural compounds OT - tumor immunogenicity COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/14 CRDT- 2026/10/28 05:37 PHST- 2026/07/21 00:00 [received] PHST- 2026/08/17 00:00 [revised] PHST- 2026/09/08 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:37 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - 1944709 [pii] AID - 10.3389/fgene.2026.1944709 [doi] PST - epublish SO - Front Genet. 2026 Oct 14;17:1944709. doi: 10.3389/fgene.2026.1944709. eCollection 2026. PMID- 42948511 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2666-2736 (Electronic) IS - 2666-2736 (Linking) VI - 33 DP - 2026 Oct TI - Low palmar arch velocity does not predict hand dysfunction after radial artery harvest: A prospective analysis of duplex ultrasonography. PG - 102065 LID - 10.1016/j.xjon.2026.102065 [doi] LID - 102065 AB - OBJECTIVES: Preoperative assessment of radial artery suitability commonly relies on the Allen test, a subjective bedside maneuver. We evaluated whether duplex ultrasonography flow metrics predict postoperative hand function after radial artery harvest for coronary artery bypass grafting. METHODS: Patients undergoing elective coronary artery bypass grafting (October 2024 to February 2026) with an appropriate second arterial conduit target underwent preoperative Allen testing and upper-extremity duplex ultrasonography, including palmar arch velocity with radial artery occlusion (PAVRO) and ulnar augmentation (percentage increase in ulnar artery flow with radial occlusion). Hand function (grip and pinch strength, fatigue, and dexterity) and patient-reported symptoms were measured preoperatively and at 1 month. Analysis of covariance and logistic regression models were adjusted for baseline hand function, age, sex, and the presence of diabetes. RESULTS: Thirty-nine patients underwent radial harvest with complete follow-up. Low PAVRO was not associated with postoperative change in any hand function metrics. Increased ulnar augmentation was associated with greater postoperative mean grip strength (P = .017) and fatigue resistance (P = .002); no patient with ulnar augmentation >40% had a clinically meaningful (>20%) decrease in grip strength. Allen test results had low concordance with duplex ultrasonography: 11 of 23 patients deemed unsuitable by duplex had a normal Allen test result at the 3-second threshold, and 13 of 47 harvested patients had an abnormal Allen test results. CONCLUSIONS: Low PAVRO does not predict hand dysfunction after radial artery harvest, whereas lower ulnar augmentation may better identify patients at risk of grip strength decrease postoperatively. Duplex ultrasonography provides objective flow metric data and should be prioritized in preoperative assessment. CI - (c) 2026 The Author(s). FAU - Szeto, Jonathan J AU - Szeto JJ AD - Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pa. FAU - Lao, James AU - Lao J AD - Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pa. FAU - Beniwal, Pamela AU - Beniwal P AD - Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pa. FAU - Song, Miranda AU - Song M AD - Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pa. FAU - Lutfi, Waseem AU - Lutfi W AD - Department of Surgery, University of Pennsylvania, Philadelphia, Pa. FAU - Xu, Victor AU - Xu V AD - Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pa. FAU - Konig, Marisa AU - Konig M AD - Division of Cardiovascular Surgery, University of Pennsylvania, Philadelphia, Pa. FAU - Pan, Ziwei AU - Pan Z AD - Division of Cardiovascular Surgery, University of Pennsylvania, Philadelphia, Pa. FAU - Kurzan, Pablo AU - Kurzan P AD - Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pa. FAU - Kapoor, Sankrit AU - Kapoor S AD - Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pa. FAU - Chung, Jennifer J AU - Chung JJ AD - Division of Cardiovascular Surgery, University of Pennsylvania, Philadelphia, Pa. FAU - Ibrahim, Michael AU - Ibrahim M AD - Division of Cardiovascular Surgery, University of Pennsylvania, Philadelphia, Pa. FAU - Szeto, Wilson Y AU - Szeto WY AD - Division of Cardiovascular Surgery, University of Pennsylvania, Philadelphia, Pa. FAU - Brown, Chase R AU - Brown CR AD - Division of Cardiovascular Surgery, University of Pennsylvania, Philadelphia, Pa. AD - Leonard Davis Institute of Health Economics, University of Pennsylvania, Philadelphia, Pa. LA - eng PT - Journal Article DEP - 20260819 PL - Netherlands TA - JTCVS Open JT - JTCVS open JID - 101768541 PMC - PMC13711214 OTO - NOTNLM OT - Allen test OT - conduit selection OT - coronary artery bypass grafting OT - coronary revascularization OT - duplex ultrasonography OT - hand function OT - radial artery harvest COIS- Dr W. Y. Szeto has consulted for Abbott, Artivion, Edwards Lifesciences, Medtronic, and Terumo Aortic, for whom he serves as an investigator, advisory board member, and speaker. All other authors reported no conflicts of interest. The Journal policy requires editors and reviewers to disclose conflicts of interest and to decline handling or reviewing manuscripts for which they may have a conflict of interest. The editors and reviewers of this article have no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/08/19 CRDT- 2026/10/28 05:36 PHST- 2026/05/11 00:00 [received] PHST- 2026/07/24 00:00 [revised] PHST- 2026/08/12 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:36 [entrez] PHST- 2026/08/19 00:00 [pmc-release] AID - S2666-2736(26)00488-2 [pii] AID - 102065 [pii] AID - 10.1016/j.xjon.2026.102065 [doi] PST - epublish SO - JTCVS Open. 2026 Aug 19;33:102065. doi: 10.1016/j.xjon.2026.102065. eCollection 2026 Oct. PMID- 42948508 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2666-2736 (Electronic) IS - 2666-2736 (Linking) VI - 33 DP - 2026 Oct TI - Hypothermic fibrillatory arrest may not increase stroke risk in minimally invasive mitral valve surgery: A 13-year propensity score-matched experience. PG - 102054 LID - 10.1016/j.xjon.2026.102054 [doi] LID - 102054 AB - OBJECTIVES: Hypothermic fibrillatory arrest (HFA) has been associated with increased stroke risk in minimally invasive mitral valve surgery, although selection bias may confound this association. METHODS: We performed a single-center retrospective analysis of minimally invasive mitral valve surgeries (July 2011 to December 2024) stratified by myocardial protection strategy (HFA vs aortic crossclamping [ACC]), assessed using propensity-score matching and multivariable logistic regression. RESULTS: Of 1258 patients, 984 underwent HFA and 274 received ACC. The fibrillatory arrest group had more complex profiles and greater rates of previous myocardial infarction (14.2% vs 4.7%), previous cardiac surgery (12.8% vs 2.2%), and urgent/emergent status (18.8% vs 10.2% all P < .05). Despite these greater-risk profiles, postoperative stroke rates were comparable (3.1% vs 3.7%, P = .57). After propensity matching, stroke remained comparable (3.3% vs 3.7%), and fibrillatory arrest was not independently associated with increased stroke risk (odds ratio, 0.94; P = .91). In-hospital mortality was also comparable (HFA: 2.6% vs ACC: 1.5%). CONCLUSIONS: HFA demonstrated noninferior stroke outcomes compared with ACC when performed with meticulous protocols at high-volume centers. These findings do not support HFA as a replacement for ACC but rather as a viable alternative when clinically and technically indicated. FAU - Ahmad, Awab AU - Ahmad A AD - Department of Cardiac Surgery, Vanderbilt University Medical Center, Nashville, Tenn. FAU - Eidson, Christian AU - Eidson C AD - Vanderbilt University School of Medicine, Nashville, Tenn. FAU - Navid, Walter AU - Navid W AD - Vanderbilt University School of Medicine, Nashville, Tenn. FAU - Stimpfl, Emma AU - Stimpfl E AD - Vanderbilt University School of Medicine, Nashville, Tenn. FAU - Wang, Chen Chia AU - Wang CC AD - Vanderbilt University School of Medicine, Nashville, Tenn. FAU - Petrovic, Mark AU - Petrovic M AD - Vanderbilt University School of Medicine, Nashville, Tenn. FAU - Williams, Aaron M AU - Williams AM AD - Department of Cardiac Surgery, Vanderbilt University Medical Center, Nashville, Tenn. FAU - Trahanas, John AU - Trahanas J AD - Department of Cardiac Surgery, Vanderbilt University Medical Center, Nashville, Tenn. FAU - Bommareddi, Swaroop AU - Bommareddi S AD - Department of Cardiac Surgery, Vanderbilt University Medical Center, Nashville, Tenn. FAU - Quintana, Eric AU - Quintana E AD - Department of Cardiac Surgery, Vanderbilt University Medical Center, Nashville, Tenn. FAU - Lima, Brian AU - Lima B AD - Department of Cardiac Surgery, Vanderbilt University Medical Center, Nashville, Tenn. FAU - Shah, Ashish S AU - Shah AS AD - Department of Cardiac Surgery, Vanderbilt University Medical Center, Nashville, Tenn. FAU - Absi, Tarek AU - Absi T AD - Department of Cardiac Surgery, Vanderbilt University Medical Center, Nashville, Tenn. LA - eng PT - Journal Article DEP - 20260822 PL - Netherlands TA - JTCVS Open JT - JTCVS open JID - 101768541 PMC - PMC13711216 OTO - NOTNLM OT - hypothermic fibrillatory arrest OT - mitral valve repair OT - mitral valve replacement OT - mitral valve surgery OT - stroke COIS- The authors reported no conflicts of interest. The Journal policy requires editors and reviewers to disclose conflicts of interest and to decline handling or reviewing manuscripts for which they may have a conflict of interest. The editors and reviewers of this article have no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/08/22 CRDT- 2026/10/28 05:36 PHST- 2026/01/26 00:00 [received] PHST- 2026/07/10 00:00 [revised] PHST- 2026/07/21 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:36 [entrez] PHST- 2026/08/22 00:00 [pmc-release] AID - S2666-2736(26)00477-8 [pii] AID - 102054 [pii] AID - 10.1016/j.xjon.2026.102054 [doi] PST - epublish SO - JTCVS Open. 2026 Aug 22;33:102054. doi: 10.1016/j.xjon.2026.102054. eCollection 2026 Oct. PMID- 42948474 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2229-3485 (Print) IS - 2229-5488 (Electronic) IS - 2229-3485 (Linking) VI - 17 IP - 4 DP - 2026 Oct-Dec TI - Description of the development and preliminary validation of antiretroviral therapy adverse event detection trigger tool for adult human immunodeficiency virus/acquired immunodeficiency syndrome patients. PG - 196-203 LID - 10.4103/picr.picr_280_25 [doi] AB - BACKGROUND: The Institute for Healthcare Improvement (IHI) Global Trigger Tool detects generic patient harm yet is not tailored to antiretroviral therapy (ART)-associated adverse drug events (ADEs) in adults living with human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome - a critical pharmacovigilance gap in care. AIM: The study is aimed at developing and preliminarily validating an HIV-specific trigger tool for ADE surveillance. MATERIALS AND METHODS: Candidate triggers were compiled from literature, expert consultation, and patient interviews, then refined through a four-round modified Delphi survey of clinicians and pharmacovigilance specialists. Inter-rater reliability (kappa) and content validity indices (CVIs) were calculated. The finalized tool was pilot-tested by retrospective review of 60 randomly selected ART patient records. RESULTS: Consensus produced 52 triggers, organized into two domains (specific and nonspecific) and 13 specific subcategories. The expert agreement was substantial (kappa =0.67). The scale-level CVI averaged 0.95. Pilot application detected 96 trigger occurrences, yielding 60 confirmed ADEs (positive predictive value = 62.5%). Switch or substitution of ART (22%) was the most common trigger, while acute kidney injury accounted for 17% of ADEs. CONCLUSION: The newly developed trigger tool demonstrates strong content validity and practical utility for identifying ART-related ADEs in adult HIV populations. Comparative studies against conventional detection methods are required to establish sensitivity and broader applicability across diverse healthcare settings. CI - Copyright: (c) 2026 Perspectives in Clinical Research. FAU - Ezeuko, Amaka Yves-Ann AU - Ezeuko AY AD - Department of Pharmacy, Drug Information Unit, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Nigeria. AD - Department of Clinical Pharmacy and Pharmacy Management, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Nigeria. FAU - Nduka, Sunday Odunke AU - Nduka SO AD - Department of Clinical Pharmacy and Pharmacy Management, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Nigeria. FAU - Oli, Angus Nnamdi AU - Oli AN AD - Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Nigeria. FAU - Chukwujekwu, Ndidiamaka Perpetua AU - Chukwujekwu NP AD - Department of Pharmacy, HIV Care Pharmacy Unit, Nnamdi Azikiwe University Teaching Hospital, Nnewi, Nigeria. FAU - Rowaiye, Adekunle Babajide AU - Rowaiye AB AD - Department of Pharmaceutical Sciences, College of Health and Sciences, North Carolina Central, University, Durham, NC, USA. FAU - Ezeuko, Kelechi Kris-Sanctus AU - Ezeuko KK AD - Department of Biochemistry, Chukwuemeka Odumegwu Ojukwu University Uli, Anambra State, Nigeria. FAU - Ekwunife, Obinna Ikechukwu AU - Ekwunife OI AD - Department of Clinical Pharmacy and Pharmacy Management, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Nigeria. LA - eng PT - Journal Article DEP - 20260220 PL - India TA - Perspect Clin Res JT - Perspectives in clinical research JID - 101551517 PMC - PMC13712627 OTO - NOTNLM OT - Adult patient OT - Delphi study OT - adverse drug event OT - antiretroviral drugs OT - antiretroviral therapy OT - trigger tools COIS- Six of the authors are affiliated to NAUTH directly or indirectly. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/01 CRDT- 2026/10/28 05:34 PHST- 2025/09/01 00:00 [received] PHST- 2025/09/08 00:00 [revised] PHST- 2025/10/28 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:34 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - PCR-17-196 [pii] AID - 10.4103/picr.picr_280_25 [doi] PST - ppublish SO - Perspect Clin Res. 2026 Oct-Dec;17(4):196-203. doi: 10.4103/picr.picr_280_25. Epub 2026 Feb 20. PMID- 42948471 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1663-9812 (Print) IS - 1663-9812 (Electronic) IS - 1663-9812 (Linking) VI - 17 DP - 2026 TI - Safety, pharmacokinetics and pharmacodynamics of ICP-332, an oral TYK2 inhibitor, in healthy participants: a randomized, double-blind, placebo-controlled phase I study. PG - 1942461 LID - 10.3389/fphar.2026.1942461 [doi] LID - 1942461 AB - OBJECTIVE: ICP-332 is a novel, orally administered TYK2 inhibitor with high selectivity, intended for the treatment of immune inflammatory diseases such as atopic dermatitis, psoriatic arthritis, inflammatory bowel disease and lupus. This Phase I study assessed its safety, pharmacokinetics, pharmacodynamics, and food effect in Chinese healthy participants. METHODS: This study was designed as a randomized, double-blind, placebo-controlled trial in healthy participants. It consisted of a single-ascending-dose (SAD) part with six cohorts (5, 15, 40, 80, 160, and 320 mg) and a multiple-ascending-dose (MAD) part with three cohorts (40, 80, and 160 mg administered once daily for 14 days). Food-effect evaluation was included at the 80 mg SAD level. In each cohort, eight participants were randomized (6:2) to receive either ICP-332 or placebo. Following oral administration, plasma samples were collected to determine ICP-332 concentrations, and safety tolerance was monitored and assessed throughout the study. RESULTS: This study enrolled 72 healthy participants, with 48 and 24 completing the SAD and MAD phases, respectively. After oral administration, ICP-332 showed rapid absorption, with median time to peak concentration (T (max)) of 0.5-2.0 h and mean elimination half-life (t ((1/2)) ) of 2.692-7.275 h. Dose-proportional exposure was confirmed in both the SAD (5-320 mg) and MAD (40-160 mg) phases; area under the concentration-time curve (AUC (last) ) ranged from 146.3 to 11,667.5 h.ng/mL in the SAD phase and from 1386.0 to 6513.5 h.ng/mL at steady state in the MAD phase. ICP-332 also induced rapid and dose-dependent suppression of TYK2 activity, as evidenced by dose-related reductions in high-sensitivity C-reactive protein and a trend toward decreased interferon gamma-induced protein 10 levels versus placebo. In terms of safety, treatment-emergent adverse events were all mild to moderate in severity, with no participant withdrawals. TEAE incidence was comparable to placebo in the SAD phase and slightly higher in the MAD phase. CONCLUSION: ICP-332 exhibited favorable safety, pharmacokinetics and pharmacodynamics profiles across the 5-320 mg dose range, with rapid absorption, dose-proportional exposure, and mild, reversible adverse events. A high-fat meal did not significantly affect its pharmacokinetics, pharmacodynamics or safety. These results support continued clinical development of ICP-332 as a promising treatment for autoimmune diseases. CLINICAL TRIAL REGISTRATION: http://www.chinadrugtrials.org.cn/index.html CTR20211781. CI - Copyright (c) 2026 Zhao, Wei, jiang, He, Zhang, Li and Wen. FAU - Zhao, Hengli AU - Zhao H AD - Department of Clinical Research Center, Central Hospital Affiliated to Shandong First Medical University, Jinan, China. FAU - Wei, Yilin AU - Wei Y AD - Department of Clinical Research Center, Central Hospital Affiliated to Shandong First Medical University, Jinan, China. FAU - Jiang, Yu AU - Jiang Y AD - Department of Clinical Research Center, Central Hospital Affiliated to Shandong First Medical University, Jinan, China. FAU - He, Kun AU - He K AD - Department of Clinical Research Center, Central Hospital Affiliated to Shandong First Medical University, Jinan, China. FAU - Zhang, Xiaoran AU - Zhang X AD - Department of Clinical Research Center, Central Hospital Affiliated to Shandong First Medical University, Jinan, China. FAU - Li, Rui AU - Li R AD - Department of Clinical Research Center, Central Hospital Affiliated to Shandong First Medical University, Jinan, China. FAU - Wen, Qing AU - Wen Q AD - Department of Clinical Research Center, Central Hospital Affiliated to Shandong First Medical University, Jinan, China. LA - eng PT - Clinical Trial PT - Journal Article DEP - 20261007 PL - Switzerland TA - Front Pharmacol JT - Frontiers in pharmacology JID - 101548923 PMC - PMC13711260 OTO - NOTNLM OT - C-reactive protein OT - ICP-332 OT - TYK2 inhibitor OT - food effect OT - pharmacodynamics OT - pharmacokinetics COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/07 CRDT- 2026/10/28 05:34 PHST- 2026/07/19 00:00 [received] PHST- 2026/08/24 00:00 [revised] PHST- 2026/08/31 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:34 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - 1942461 [pii] AID - 10.3389/fphar.2026.1942461 [doi] PST - epublish SO - Front Pharmacol. 2026 Oct 7;17:1942461. doi: 10.3389/fphar.2026.1942461. eCollection 2026. PMID- 42948460 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2042-8812 (Print) IS - 2042-8812 (Electronic) IS - 2042-8812 (Linking) VI - 2026 IP - 10 DP - 2026 Oct TI - Arteriovenous malformation (hemangioma) of the radial artery: case report and treatment outcome. PG - rjag936 LID - 10.1093/jscr/rjag936 [doi] LID - rjag936 AB - Arteriovenous malformation hemangioma (AVMH) is a rare benign vascular lesion typically diagnosed in young adults, with 80%-90% of patients presenting before the age of 30. It most commonly affects the extremities, followed by the head and neck region. Establishing the differential diagnosis of AVMH can be challenging because it must be distinguished from other soft tissue lesions of the extremities. We present the case of a 44-year-old woman with recurrent AVMH, successfully treated with foam sclerotherapy using 10 ml of 3% polidocanol (Ethoxysclerol), prepared according to the Tessari method, followed by ligation of the radial artery. This report suggests that this combined therapeutic approach may represent an effective treatment option for selected patients with recurrent or extensive lesions when complete surgical excision is difficult or associated with significant morbidity. CI - (c) The Author(s) 2026. Published by Oxford University Press and JSCR Publishing Ltd. FAU - Todorova, Teodora AU - Todorova T AUID- ORCID: 0009-0005-3728-9682 AD - University Clinic for Orthopedic Surgery, Mother Teresa No. 17, Skopje 1000, North Macedonia. FAU - Angelovska, Tamara AU - Angelovska T AD - Faculty of Medicine, Institute of Pathology, University Ss. Cyril and Methodius in Skopje, 50 Divizija No. 6, Skopje 1000, North Macedonia. FAU - Kostadinova-Kunovska, Slavica AU - Kostadinova-Kunovska S AD - Faculty of Medicine, Institute of Pathology, University Ss. Cyril and Methodius in Skopje, 50 Divizija No. 6, Skopje 1000, North Macedonia. FAU - Gramatnikovski, Nikola AU - Gramatnikovski N AD - University Clinic for Thoracic and Vascular Surgery, Skopje 1000, North Macedonia. FAU - Daskalov, Dejan AU - Daskalov D AD - Institute of Radiology, Faculty of Medicine, Ss. Cyril and Methodius University in Skopje, North Macedonia. FAU - Foteva, Marta AU - Foteva M AD - University Clinic for Orthopedic Surgery, Mother Teresa No. 17, Skopje 1000, North Macedonia. FAU - Velkovski, Vilijam AU - Velkovski V AD - University Clinic for Orthopedic Surgery, Mother Teresa No. 17, Skopje 1000, North Macedonia. FAU - Atanasov, Nenad AU - Atanasov N AD - University Clinic for Orthopedic Surgery, Mother Teresa No. 17, Skopje 1000, North Macedonia. FAU - Andonovski, Alan AU - Andonovski A AD - University Clinic for Orthopedic Surgery, Mother Teresa No. 17, Skopje 1000, North Macedonia. FAU - Samardziski, Milan AU - Samardziski M AD - University Clinic for Orthopedic Surgery, Mother Teresa No. 17, Skopje 1000, North Macedonia. LA - eng PT - Case Reports PT - Journal Article DEP - 20261027 PL - England TA - J Surg Case Rep JT - Journal of surgical case reports JID - 101560169 PMC - PMC13712507 OTO - NOTNLM OT - arteriovenous malformation OT - hemangioma OT - sclerotherapy COIS- None declared. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/27 CRDT- 2026/10/28 05:33 PHST- 2026/08/10 00:00 [received] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:33 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - rjag936 [pii] AID - 10.1093/jscr/rjag936 [doi] PST - epublish SO - J Surg Case Rep. 2026 Oct 27;2026(10):rjag936. doi: 10.1093/jscr/rjag936. eCollection 2026 Oct. PMID- 42948459 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2666-2736 (Electronic) IS - 2666-2736 (Linking) VI - 33 DP - 2026 Oct TI - Minimally invasive mitral surgery in patients with high pulmonary artery pressure: Results of a multicenter observational study. PG - 101966 LID - 10.1016/j.xjon.2026.101966 [doi] LID - 101966 AB - OBJECTIVES: Pulmonary hypertension is diagnosed in 40% to 75% of patients affected by mitral valve diseases. Little is known regarding outcomes of patients with pulmonary hypertension undergoing mitral valve oeprations through minimally invasive approaches. This study compares the characteristics and in-hospital outcomes of patients undergoing minimally invasive mitral valve surgery, stratified by systolic pulmonary artery pressure. METHODS: This multicenter, retrospective, prospective, observational study included adults undergoing mitral valve operations through a right minithoracotomy with or without tricuspid valve surgery and atrial fibrillation ablation between 2020 and 2024. Comparisons were performed between patients with systolic pulmonary artery pressure less than 31 mm Hg, systolic pulmonary artery pressure 31 to 55 mm Hg, and systolic pulmonary artery pressure more than 55 mm Hg based on European System for Cardiac Operative Risk Evaluation II cutoffs for systolic pulmonary artery pressure categories. RESULTS: This analysis included 1121 patients (female, 47.5%; median age, 69 years, interquartile range, 59-77). Median systolic pulmonary artery pressure was 38 mm Hg (interquartile range, 30-50; systolic pulmonary artery pressure <31 mm Hg: n = 307, 27.4%; systolic pulmonary artery pressure 31-55 mm Hg: n = 152, 55.3%; systolic pulmonary artery pressure >55 mm Hg: n = 194, 17.3%). Patients with higher systolic pulmonary artery pressure were older, mainly female, and characterized by more comorbidities including atrial fibrillation and renal disease. A critical perioperative state was diagnosed in 12% of patients with systolic pulmonary artery pressure more than 55 mm Hg (P < .001). Patients with higher systolic pulmonary artery pressure more frequently underwent mitral valve replacement and tricuspid annuloplasty and experienced more postoperative dialysis and pacemaker implantation but comparable rates of conversions to sternotomy, respiratory and bleeding complications, and in-hospital mortality (systolic pulmonary artery pressure <31 mm Hg: n = 3, 1%; systolic pulmonary artery pressure 31-55 mm Hg: n = 6, 1%; systolic pulmonary artery pressure >55 mm Hg: n = 5, 2.6%; P = .222) compared with patients with lower systolic pulmonary artery pressure. CONCLUSIONS: Despite a higher preoperative complexity, patients with high systolic pulmonary artery pressure could be considered for minimally invasive mitral valve surgery with acceptable rates of major intraoperative or postoperative complications regardless of their systolic pulmonary artery pressure values. CI - (c) 2026 The Author(s). FAU - Mariani, Silvia AU - Mariani S AD - Cardiac Surgery Unit, Cardiothoracic and Vascular Department, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy. AD - Cardiovascular Research Institute Maastricht, University of Maastricht, Maastricht, The Netherlands. FAU - Barbero, Cristina AU - Barbero C AD - Department of Surgical Sciences, University of Turin, Turin, Italy. FAU - Gislao, Valentina AU - Gislao V AD - Cardiac Surgery Unit, Cardiothoracic and Vascular Department, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy. FAU - Capozza, Cecilia AU - Capozza C AD - Cardiac Surgery Unit, Cardiothoracic and Vascular Department, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy. FAU - Stefano, Pierluigi AU - Stefano P AD - Cardiac Surgery Unit, Department of Cardiothoracic and Vascular Surgery, Careggi University Hospital, Florence, Italy. FAU - Centofanti, Paolo AU - Centofanti P AD - Department of Cardiac Surgery, Azienda Ospedaliera Ordine Mauriziano di Torino, Turin, Italy. FAU - Lodo, Vittoria AU - Lodo V AD - Department of Cardiac Surgery, Azienda Ospedaliera Ordine Mauriziano di Torino, Turin, Italy. FAU - Onorati, Francesco AU - Onorati F AD - Division of Cardiac Surgery, University of Trento, ASUIT, Trento, Italy. FAU - Cibin, Giorgia AU - Cibin G AD - Division of Cardiac Surgery, University of Trento, ASUIT, Trento, Italy. FAU - Di Credico, Germano AU - Di Credico G AD - Department of Cardiac Surgery, ASST Ovest Milanese, Legnano, Italy. FAU - Audo, Andrea AU - Audo A AD - Cardiac Surgery Department, University Hospital "SS. Antonio e Biagio e C. Arrigo", Alessandria, Italy. FAU - De Paulis, Ruggero AU - De Paulis R AD - Department of Cardiac Surgery, European Hospital, Unicamillus University, Rome, Italy. FAU - Rinaldi, Mauro AU - Rinaldi M AD - Department of Surgical Sciences, University of Turin, Turin, Italy. FAU - Marchetto, Giovanni AU - Marchetto G AD - Cardiac Surgery Unit, Cardiothoracic and Vascular Department, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy. AD - School of Medicine and Surgery, University of Milano - Bicocca, Milan, Italy. CN - Minimally Invasive Mitral Valve Surgery Registry Investigators LA - eng PT - Journal Article DEP - 20260716 PL - Netherlands TA - JTCVS Open JT - JTCVS open JID - 101768541 PMC - PMC13711211 OTO - NOTNLM OT - heart failure OT - minimally invasive OT - mitral valve surgery OT - pulmonary hypertension COIS- F.O.: research grant and speaker fees from Edwards Lifesciences; speaker fees from Abbott. All other authors reported no conflicts of interest. The Journal policy requires editors and reviewers to disclose conflicts of interest and to decline handling or reviewing manuscripts for which they may have a conflict of interest. The editors and reviewers of this article have no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/07/16 CRDT- 2026/10/28 05:33 PHST- 2026/03/12 00:00 [received] PHST- 2026/05/23 00:00 [revised] PHST- 2026/07/06 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:33 [entrez] PHST- 2026/07/16 00:00 [pmc-release] AID - S2666-2736(26)00389-X [pii] AID - 101966 [pii] AID - 10.1016/j.xjon.2026.101966 [doi] PST - epublish SO - JTCVS Open. 2026 Jul 16;33:101966. doi: 10.1016/j.xjon.2026.101966. eCollection 2026 Oct. PMID- 42948457 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1831-4732 (Electronic) IS - 1831-4732 (Linking) VI - 24 IP - 10 DP - 2026 Oct TI - Guidance on default values and uncertainty factors to be used by the EFSA Scientific Committee, Scientific Panels and Units in the absence of actual measured data. PG - e10391 LID - 10.2903/j.efsa.2026.10391 [doi] LID - e10391 AB - The European Food Safety Authority (EFSA) has updated its 2012 guidance on selected default values (DVs) to be used by the EFSA Scientific Committee (SC), Scientific Panels and Units in the absence of actual measured data. DVs are meant to be typical values, being not necessarily conservative, while uncertainty factors (UFs) tend to be conservative. Examples of DVs are for body weights of humans or animal species, food and feed intake, or conversion factors for chemical substance concentration in feed or drinking water into daily doses in experimental animal studies. UFs are used to account for uncertainties in the assessment e.g. regarding inter/intra-species extrapolation, extrapolation for duration of exposure or for deficiencies in the database. The SC assessed the scientific basis for the DVs and UFs used by EFSA Units/Panels, considering published literature and the evidence derived from the available scientific databases. Most of the proposed values in the 2012 SC guidance and the rules for rounding of derived values were retained, while some DVs had become obsolete. The main changes concern newly added DVs and the updated scientific rationale for a number of the existing DVs and UFs. Regarding the UF of 100 for inter/intra-species extrapolation, the SC recommends that EFSA convenes an international expert group to evaluate the basis of the UF of 100 and its sub-factors for inter/intra-species extrapolation. In addition, the use of probabilistic approaches should be investigated. The SC reiterates that, whenever specific robust and reliable data are available, these should be used instead of DVs. The aim of this guidance document is not standardisation but harmonisation. It is always possible to deviate from the proposed DVs and UFs, provided that the rationale for such deviation is described. CI - (c) 2026 European Food Safety Authority. EFSA Journal published by Wiley-VCH GmbH on behalf of European Food Safety Authority. CN - EFSA Scientific Committee (SC) FAU - Bennekou, Susanne Hougaard AU - Bennekou SH FAU - Allende, Ana AU - Allende A FAU - Bearth, Angela AU - Bearth A FAU - Casacuberta, Josep AU - Casacuberta J FAU - Castle, Laurence AU - Castle L FAU - Coja, Tamara AU - Coja T FAU - Crepet, Amelie AU - Crepet A FAU - Halldorsson, Thorhallur AU - Halldorsson T FAU - Hoogenboom, Laurentius Ron AU - Hoogenboom LR FAU - Jokelainen, Pikka AU - Jokelainen P FAU - Knutsen, Helle AU - Knutsen H FAU - Lambre, Claude AU - Lambre C FAU - Nielsen, Soren AU - Nielsen S FAU - Turck, Dominique AU - Turck D FAU - Civera, Antonio Vicent AU - Civera AV FAU - Villa, Roberto Edoardo AU - Villa RE FAU - Zorn, Holger AU - Zorn H FAU - Machera, Kyriaki AU - Machera K FAU - Schlatter, Josef AU - Schlatter J FAU - Anastassiadou, Maria AU - Anastassiadou M FAU - Buzle, Dayana AU - Buzle D FAU - De Sesmaisons-Lecarre, Agnes AU - De Sesmaisons-Lecarre A FAU - Dorne, Jean-Lou AU - Dorne JL FAU - Gergelova, Petra AU - Gergelova P LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - EFSA J JT - EFSA journal. European Food Safety Authority JID - 101642076 PMC - PMC13711252 OTO - NOTNLM OT - body weight OT - conversion factors OT - default values OT - food and feed intake OT - human milk intake OT - rounding of figures OT - uncertainty factors EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/27 CRDT- 2026/10/28 05:33 PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:33 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - EFS210391 [pii] AID - 10.2903/j.efsa.2026.10391 [doi] PST - epublish SO - EFSA J. 2026 Oct 27;24(10):e10391. doi: 10.2903/j.efsa.2026.10391. eCollection 2026 Oct. PMID- 42948453 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2666-2736 (Electronic) IS - 2666-2736 (Linking) VI - 33 DP - 2026 Oct TI - Diagnosis and minimally invasive surgical treatment of peritoneal dialysis-associated pleuroperitoneal fistula: A case report and literature review. PG - 101951 LID - 10.1016/j.xjon.2026.101951 [doi] LID - 101951 FAU - Zhao, Yangle AU - Zhao Y AD - Department of Thoracic Surgery, Chuiyangliu Hospital Affiliated with Tsinghua University, Beijing, China. FAU - Zhang, Enxiang AU - Zhang E AD - Department of Nephrology, Chuiyangliu Hospital Affiliated with Tsinghua University, Beijing, China. FAU - Hu, Xiaodan AU - Hu X AD - Department of Thoracic Surgery, Chuiyangliu Hospital Affiliated with Tsinghua University, Beijing, China. FAU - Wang, Xiao AU - Wang X AD - Department of Thoracic Surgery, Chuiyangliu Hospital Affiliated with Tsinghua University, Beijing, China. FAU - Zhao, Yun AU - Zhao Y AD - Department of Thoracic Surgery, Chuiyangliu Hospital Affiliated with Tsinghua University, Beijing, China. FAU - Yu, Yongwu AU - Yu Y AD - Department of Nephrology, Chuiyangliu Hospital Affiliated with Tsinghua University, Beijing, China. FAU - Cui, Jian AU - Cui J AD - Department of Thoracic Surgery, Chuiyangliu Hospital Affiliated with Tsinghua University, Beijing, China. LA - eng PT - Case Reports PT - Journal Article DEP - 20260704 PL - Netherlands TA - JTCVS Open JT - JTCVS open JID - 101768541 PMC - PMC13711202 COIS- The authors reported no conflicts of interest. The Journal policy requires editors and reviewers to disclose conflicts of interest and to decline handling or reviewing manuscripts for which they may have a conflict of interest. The editors and reviewers of this article have no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/07/04 CRDT- 2026/10/28 05:33 PHST- 2026/03/21 00:00 [received] PHST- 2026/05/26 00:00 [revised] PHST- 2026/06/12 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:33 [entrez] PHST- 2026/07/04 00:00 [pmc-release] AID - S2666-2736(26)00374-8 [pii] AID - 101951 [pii] AID - 10.1016/j.xjon.2026.101951 [doi] PST - epublish SO - JTCVS Open. 2026 Jul 4;33:101951. doi: 10.1016/j.xjon.2026.101951. eCollection 2026 Oct. PMID- 42948450 OWN - NLM STAT- Publisher LR - 20261029 IS - 2212-6287 (Print) IS - 2212-6287 (Electronic) IS - 2212-6287 (Linking) DP - 2026 Oct 27 TI - Arthroscopic Technique for Anterior Calcaneal Process Avulsion Fractures and Bifurcate Ligament Injuries Using Suture Tape Augmentation. PG - e70330 LID - 10.1002/atn2.70330 [doi] LID - e70330 AB - Avulsion fractures of the anterior process of the calcaneus are frequently associated with injury to the bifurcate ligament and may result in persistent lateral midfoot pain and instability. Although open fixation or fragment excision has traditionally been used, these approaches may fail to restore ligamentous function and joint stability. Arthroscopic techniques for combined management of bony and ligamentous pathology at the calcaneocuboid joint remain poorly described. We describe an arthroscopic technique for the management of anterior calcaneal process avulsion fractures associated with bifurcate ligament injury. The procedure combines endoscopic excision of symptomatic intra-articular fragments with internal brace augmentation of both limbs of the bifurcate ligament using suture tape fixation, preserving residual native fibers and restoring ligamentous stability. This arthroscopic technique allows minimally invasive management of combined osseous and ligamentous pathology of the calcaneocuboid joint, providing stable ligament augmentation while avoiding the morbidity of open surgery and rigid across-joint fixation. Arthroscopic bifurcate ligament internal brace augmentation may represent a valuable option for selected patients with symptomatic anterior calcaneal process avulsion fractures and associated midfoot instability. CI - (c) 2026 The Author(s). Arthroscopy Techniques published by Wiley Periodicals LLC on behalf of the Arthroscopy Association of North America. FAU - Diaz-Fernandez, Rodrigo AU - Diaz-Fernandez R AUID- ORCID: 0000-0003-0591-1084 AD - Faculty of Medicine Universidad Catolica de Valencia "San Vicente Martir" Valencia Spain. AD - Foot and Ankle Unit Hospital de Manises Valencia Spain. AD - Foot and Ankle Unit Hospital Vithas Turia Valencia Spain. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Arthrosc Tech JT - Arthroscopy techniques JID - 101597442 PMC - PMC13712393 EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/27 CRDT- 2026/10/28 05:33 PHST- 2026/02/19 00:00 [received] PHST- 2026/06/16 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:33 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - ATN270330 [pii] AID - 10.1002/atn2.70330 [doi] PST - aheadofprint SO - Arthrosc Tech. 2026 Oct 27:e70330. doi: 10.1002/atn2.70330. PMID- 42948436 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-302X (Print) IS - 1664-302X (Electronic) IS - 1664-302X (Linking) VI - 17 DP - 2026 TI - Halite crystallization at Sfax solar salterns, Tunisia, driven by brine geochemistry and biogeochemical interactions. PG - 1843317 LID - 10.3389/fmicb.2026.1843317 [doi] LID - 1843317 AB - Solar salterns are hypersaline systems characterized by evaporation-driven salinity gradients that promote the precipitation of evaporite minerals. Here, we investigate the influence of halophilic microbial communities on halite crystallization along the salinity gradient of the Sfax solar saltern (Tunisia). Geochemical analyses of 26 brine samples (mean salinity range ~28-570 g L(-1)) demonstrated marine-fed evaporative sequences, allowing the selection of three representative basins along a gradient of 150 g/L to 280 g/L (M22, B22, and TS5 sites). Enrichment cultures inoculated with surface (SB) and bottom (BB) brines were established under conditions reproducing in situ brine chemistry. Three halophilic archaeal species, Halorubrum xinjiangense, H. africanae, and H. ezzemoulense, were isolated from cultures able to tolerate salinities of up to 280 g L(-1). Subsequently, controlled crystallization experiments using high-salinity biomass as a progressively increasing inoculum revealed that biomass-associated microbial populations influenced halite precipitation kinetics, yield, and crystal morphology relative to abiotic controls. SB-derived cultures reached maximum halite-dominated precipitate mas at a lower biomass level (25% inoculum), yielding 5.550 +/- 0.127 g after 10 days at 0.555 +/- 0.013 g d(-1), whereas BB-derived cultures peaked at 75% inoculum (64.20 +/- 2.632 mg mL(-1)), producing 3.570 +/- 0.085 g after 12 days at 0.299 +/- 0.028 g d(-1). This contrast shows that biomass affects crystallization differently in surface- and bottom-derived cultures, with differences in crystal size, shape, aggregation, and uniformity. These results demonstrate that halophilic microorganisms could modulate halite crystallization under extreme salinity, highlighting the importance of microbial processes in evaporite mineralization within hypersaline environments. CI - Copyright (c) 2026 Gharsalli, Mhiri, Yaich and Karray. FAU - Gharsalli, Najoua AU - Gharsalli N AD - Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, Sfax, Tunisia. AD - Laboratory of Environmental Engineering and Ecotechnology (GEET), University of Sfax, Sfax, Tunisia. FAU - Mhiri, Najla AU - Mhiri N AD - Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, Sfax, Tunisia. FAU - Yaich, Chokri AU - Yaich C AD - Laboratory of Georesources, Materials, Environment and Global Change (GEOGLOB), Sfax, Tunisia. AD - National Engineering School of Sfax (ENIS), University of Sfax, Sfax, Tunisia. FAU - Karray, Fatma AU - Karray F AD - Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, Sfax, Tunisia. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Microbiol JT - Frontiers in microbiology JID - 101548977 PMC - PMC13712413 OTO - NOTNLM OT - archaea OT - biogeochemical interactions OT - biomineralization OT - brine geochemistry OT - halite crystallization OT - hypersaline environments COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:32 PHST- 2026/03/31 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:32 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fmicb.2026.1843317 [doi] PST - epublish SO - Front Microbiol. 2026 Oct 13;17:1843317. doi: 10.3389/fmicb.2026.1843317. eCollection 2026. PMID- 42948434 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1873-9601 (Print) IS - 1873-961X (Electronic) IS - 1873-9601 (Linking) VI - 20 IP - 4 DP - 2026 Dec TI - Systems-level phosphoproteomics reveals calcium/calmodulin-dependent protein kinase kinase 2-driven kinase signaling underlying malignant phenotypes in gastric cancer. PG - e70112 LID - 10.1002/ccs3.70112 [doi] LID - e70112 AB - Gastric cancer (GC) is driven by aberrant kinase signaling that promotes uncontrolled proliferation and malignant progression. Calcium/calmodulin-dependent protein kinase kinase 2 (CAMKK2) is overexpressed in GC; however, the global phosphorylation networks downstream of CAMKK2 remain incompletely defined. In this study, we investigated the functional and signaling consequences of CAMKK2 inhibition in GC cells using an integrated phenotypic and quantitative phosphoproteomics approach. Pharmacological inhibition of CAMKK2 using STO-609 in AGS cells significantly suppressed proliferation, clonogenic growth, migration, and invasion and induced defects in nuclear morphology indicative of impaired cell cycle progression. Tandem mass tag-based phosphoproteomic profiling identified over 10,500 phosphopeptides and revealed extensive phosphoproteome remodeling after CAMKK2 inhibition, characterized predominantly by hypophosphorylation of proteins involved in nuclear signaling, RNA processing, and cell cycle regulation. Kinase-substrate enrichment and motif analyses demonstrated coordinated attenuation of cyclin-dependent kinase, mitogen-activated protein kinase, and mitotic kinase-associated signaling pathways, with convergence on E2F-regulated transcriptional programs. Collectively, these findings establish CAMKK2 as a central regulator of kinase signaling networks that sustain proliferative and malignant phenotypes in GC and highlight CAMKK2 inhibition as a potential therapeutic strategy. CI - (c) 2026 The Author(s). Journal of Cell Communication and Signaling published by John Wiley & Sons Ltd. FAU - Najar, Mohd Altaf AU - Najar MA AUID- ORCID: 0000-0002-3044-3772 AD - Center for Systems Biology and Molecular Medicine Yenepoya (Deemed to be University) Mangalore India. FAU - Prasad, T S Keshava AU - Prasad TSK AUID- ORCID: 0000-0002-6206-2384 AD - Center for Systems Biology and Molecular Medicine Yenepoya (Deemed to be University) Mangalore India. AD - Department of Biochemistry Nitte (Deemed to be University) Center for Omics & Systems Medicine (C-OSM) K.S. Hegde Medical Academy (KSHEMA) Mangalore Karnataka India. FAU - Modi, Prashant Kumar AU - Modi PK AUID- ORCID: 0000-0002-4817-3379 AD - Center for Systems Biology and Molecular Medicine Yenepoya (Deemed to be University) Mangalore India. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Cell Commun Signal JT - Journal of cell communication and signaling JID - 101308338 PMC - PMC13712449 OTO - NOTNLM OT - CAMKK2 OT - MAPK signaling OT - RNA metabolism OT - cell cycle regulation OT - gastric cancer OT - kinase signaling OT - phosphoproteomics OT - systems biology COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/27 CRDT- 2026/10/28 05:32 PHST- 2026/05/18 00:00 [received] PHST- 2026/08/06 00:00 [revised] PHST- 2026/08/17 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:32 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - CCS370112 [pii] AID - 10.1002/ccs3.70112 [doi] PST - epublish SO - J Cell Commun Signal. 2026 Oct 27;20(4):e70112. doi: 10.1002/ccs3.70112. eCollection 2026 Dec. PMID- 42948431 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2666-2736 (Electronic) IS - 2666-2736 (Linking) VI - 33 DP - 2026 Oct TI - Pericardial fluid cardiac troponin I predicts postoperative atrial fibrillation after coronary artery bypass grafting. PG - 101954 LID - 10.1016/j.xjon.2026.101954 [doi] LID - 101954 AB - OBJECTIVE: Cardiac troponin I (cTnI) is a sensitive biomarker of myocardial injury. We hypothesized that cTnI measured in pericardial drainage fluid (PCF), which reflects the cardiac microenvironment, may enhance risk stratification for postoperative atrial fibrillation (POAF) after coronary artery bypass grafting. METHODS: In this prospective cohort study, 114 patients undergoing elective coronary artery bypass grafting were enrolled. The primary end point was new-onset POAF within 7 days postoperatively. Blood and PCF cTnI at 6 hours postoperatively were measured. Variables were screened using least absolute shrinkage and selection operator regression and were then incorporated into multivariable logistic regression to construct a predictive model. A simplified risk score (CALT score) was developed. Model performance was compared with existing POAF tools, including the POAF score, CHA(2)DS(2)-VASc, and the atrial fibrillation risk index. RESULTS: POAF occurred in 30.7% of the cohort. Postoperative 6-hour PCF-cTnI was an independent predictor of POAF (odds ratio, 5.502; 95% CI, 1.887-16.045; P = .002) and significantly enhanced continuous net reclassification improvement (0.562; 95% CI, 0.189-0.921; P = .002). The CALT score comprised left circumflex artery lesions, left atrial diameter, left ventricular end-diastolic diameter, postoperative 0-hour serum calcium, and postoperative 6-hour PCF-cTnI. The PCF-cTnI-based model demonstrated good discrimination (area under the receiver operating characteristic curve = 0.821), with favorable calibration and clinical utility. Compared with commonly used risk scores, the CALT score exhibited superior discrimination and calibration. CONCLUSIONS: This study provides novel evidence that PCF-cTnI is a strong predictor of POAF. The CALT score, integrating PCF-cTnI with clinical factors, provided superior predictive performance by capturing both the vulnerable atrial substrate and localized myocardial injury. CI - (c) 2026 The Author(s). FAU - Shu, Yue AU - Shu Y AD - School of Nursing, Capital Medical University, Beijing, P. R. China. FAU - Gao, Chaoyue AU - Gao C AD - School of Nursing, Capital Medical University, Beijing, P. R. China. FAU - Ren, Xiaoshuang AU - Ren X AD - Department of Cardiac Surgery Intensive Care Unit (ICU) 4, Beijing Anzhen Hospital, Capital Medical University, Beijing, P. R. China. FAU - Deng, Xun AU - Deng X AD - Department of Cardiac Surgery Intensive Care Unit (ICU) 1, Beijing Anzhen Hospital, Capital Medical University, Beijing, P. R. China. FAU - Luo, Lin AU - Luo L AD - School of Nursing, Capital Medical University, Beijing, P. R. China. FAU - Wang, Taiwei AU - Wang T AD - School of Nursing, Capital Medical University, Beijing, P. R. China. FAU - Ren, Zexinyao AU - Ren Z AD - School of Nursing, Capital Medical University, Beijing, P. R. China. FAU - Zhao, Jiahui AU - Zhao J AD - School of Nursing, Capital Medical University, Beijing, P. R. China. FAU - Liu, Yisi AU - Liu Y AD - School of Nursing, Capital Medical University, Beijing, P. R. China. LA - eng PT - Journal Article DEP - 20260706 PL - Netherlands TA - JTCVS Open JT - JTCVS open JID - 101768541 PMC - PMC13711195 OTO - NOTNLM OT - cardiac troponin I OT - coronary artery bypass grafting OT - pericardial fluid OT - postoperative atrial fibrillation OT - risk prediction model COIS- The authors reported no conflicts of interest. The Journal policy requires editors and reviewers to disclose conflicts of interest and to decline handling or reviewing manuscripts for which they may have a conflict of interest. The editors and reviewers of this article have no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/07/06 CRDT- 2026/10/28 05:32 PHST- 2026/02/08 00:00 [received] PHST- 2026/06/11 00:00 [revised] PHST- 2026/06/29 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:32 [entrez] PHST- 2026/07/06 00:00 [pmc-release] AID - S2666-2736(26)00377-3 [pii] AID - 101954 [pii] AID - 10.1016/j.xjon.2026.101954 [doi] PST - epublish SO - JTCVS Open. 2026 Jul 6;33:101954. doi: 10.1016/j.xjon.2026.101954. eCollection 2026 Oct. PMID- 42948426 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2211-3835 (Print) IS - 2211-3843 (Electronic) IS - 2211-3835 (Linking) VI - 16 IP - 10 DP - 2026 Oct TI - BD2-selective BRD4 inhibitor ZX29 mitigates myocardial ischemia-reperfusion injury via BRD4-YY1-NCOA4 axis disruption and ferroptosis inhibition. PG - 6731-6755 LID - 10.1016/j.apsb.2026.07.043 [doi] AB - Myocardial ischemia/reperfusion injury (MI/RI) remains a major barrier to ischemic heart disease treatment, as reperfusion therapies often exacerbate cardiac damage. Bromodomain-containing protein 4 (BRD4) is a key epigenetic target in cardiovascular diseases, but pan-BRD4 inhibitors cause severe off-target toxicity by disrupting basal bromodomain 1 (BD1) function, highlighting the need for BD2-selective agents. This study aimed to develop a BD2-selective BRD4 inhibitor and clarify its mechanism. ZX29 was developed via virtual screening of 100,000 compounds and structure-based optimization, exhibiting potent BRD4 binding with an IC(50) of 0.27 +/- 0.03 mumol/L and K (d) of 1.68 mumol/L, outperforming clinical agent RVX-208. ZX29 specifically binds BRD4 BD2 via H437/V439 residues. In murine MI/RI models, oral ZX29 significantly improved cardiac systolic and diastolic function, reduced myocardial infarct size, and mitigated lipid peroxidation. Mechanistically, ZX29 disrupted the BRD4-Yin Yang 1 (YY1) interaction and inhibited the nuclear receptor coactivator 4 (NCOA4)-ferritin heavy chain 1 (FTH1) degradation axis, suppressing lysosomal ferrous iron release and ferroptosis. Moreover, ZX29 preserved mitochondrial function by maintaining mitochondrial membrane potential and boosting ATP production. Ultimately, this study validates selective BRD4 BD2 inhibition as a safe and effective therapeutic strategy for MI/RI and establishes ZX29 as a viable clinical candidate. CI - (c) 2026 The Authors. FAU - Li, Zeyu AU - Li Z AD - National and Local United Engineering Lab of Druggability and New Drugs Evaluation, Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, Guangdong Province Engineering Laboratory for Druggability and New Drug Evaluation, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. AD - Department of Cardiac Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China. FAU - Xin, Meixiu AU - Xin M AD - National and Local United Engineering Lab of Druggability and New Drugs Evaluation, Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, Guangdong Province Engineering Laboratory for Druggability and New Drug Evaluation, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. FAU - Wu, Zhongkai AU - Wu Z AD - Department of Cardiac Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China. FAU - Lu, Jing AU - Lu J AD - National and Local United Engineering Lab of Druggability and New Drugs Evaluation, Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, Guangdong Province Engineering Laboratory for Druggability and New Drug Evaluation, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. FAU - Hu, Ou AU - Hu O AD - School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. FAU - Zou, Yong AU - Zou Y AD - National and Local United Engineering Lab of Druggability and New Drugs Evaluation, Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, Guangdong Province Engineering Laboratory for Druggability and New Drug Evaluation, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. FAU - Liu, Peiqing AU - Liu P AD - National and Local United Engineering Lab of Druggability and New Drugs Evaluation, Guangdong Provincial Key Laboratory of New Drug Design and Evaluation, Guangdong Province Engineering Laboratory for Druggability and New Drug Evaluation, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China. LA - eng PT - Journal Article DEP - 20260730 PL - Netherlands TA - Acta Pharm Sin B JT - Acta pharmaceutica Sinica. B JID - 101600560 PMC - PMC13712366 OTO - NOTNLM OT - BRD4 OT - Cardiac fibrosis OT - Epigenetic regulation OT - Ferroptosis OT - Lipid peroxidation OT - Mitochondrial dysfunction OT - Myocardial injury OT - YY1 COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/07/30 CRDT- 2026/10/28 05:32 PHST- 2025/09/19 00:00 [received] PHST- 2026/03/15 00:00 [revised] PHST- 2026/03/18 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:32 [entrez] PHST- 2026/07/30 00:00 [pmc-release] AID - S2211-3835(26)00485-5 [pii] AID - 10.1016/j.apsb.2026.07.043 [doi] PST - ppublish SO - Acta Pharm Sin B. 2026 Oct;16(10):6731-6755. doi: 10.1016/j.apsb.2026.07.043. Epub 2026 Jul 30. PMID- 42948425 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2666-2736 (Electronic) IS - 2666-2736 (Linking) VI - 33 DP - 2026 Oct TI - Evaluating preoperative imaging in patients with chronic thromboembolic pulmonary hypertension: Is computed tomography pulmonary angiography a reliable alternative to pulmonary angiography? PG - 101960 LID - 10.1016/j.xjon.2026.101960 [doi] LID - 101960 AB - OBJECTIVES: Chronic thromboembolic pulmonary hypertension is a potentially curable form of pulmonary hypertension, with pulmonary endarterectomy serving as a key surgical treatment option. Accurate preoperative imaging is critical for determining surgical candidacy and optimizing operative outcomes. Although digital subtraction pulmonary angiography remains the gold standard, computed tomography pulmonary angiography has emerged as a noninvasive alternative. This study compares the concordance of computed tomography pulmonary angiography and digital subtraction pulmonary angiography with intraoperative specimen findings to determine their diagnostic accuracy. METHODS: We conducted a retrospective cohort study of patients who underwent pulmonary endarterectomy for chronic thromboembolic pulmonary hypertension at a single Canadian center between 2014 and 2017. All patients underwent preoperative computed tomography pulmonary angiography and digital subtraction pulmonary angiography as part of their routine evaluation. Imaging findings were compared with operative findings using a territory-based anatomic assessment across major pulmonary artery segments. Functional and hemodynamic outcomes were also assessed before and after surgery. RESULTS: Seventeen patients (mean age 52.8 years [+/-3.7]; 53% female) were included. Computed tomography pulmonary angiography demonstrated greater agreement with operative findings in the right main pulmonary artery, left upper lobe, and lingula. Digital subtraction pulmonary angiography demonstrated greater agreement in the right upper lobe, whereas both modalities showed similar agreement in the left main pulmonary artery and left lower lobe. Patients experienced postoperative improvements in New York Heart Association functional class, pulmonary artery pressures, and cardiac index. Despite overall agreement between imaging and operative findings, discrepancies persisted across several vascular territories. CONCLUSIONS: Computed tomography pulmonary angiography demonstrated substantial agreement with operative findings in central and lobar pulmonary arterial territories, supporting its role as an important frontline imaging modality in the evaluation of chronic thromboembolic pulmonary hypertension. However, persistent discrepancies between imaging and operative assessment, particularly in more distal territories, support the continued use of a multimodal approach integrating computed tomography pulmonary angiography, digital subtraction pulmonary angiography, hemodynamic assessment, and multidisciplinary evaluation when determining operability and treatment strategy. CI - (c) 2026 The Author(s). FAU - Alamri, Rawan AU - Alamri R AD - Division of Cardiac Surgery, Jewish General Hospital, McGill University, Montreal, Quebec, Canada. FAU - Majeed, Hamnah AU - Majeed H AD - Division of Cardiac Surgery, University of Toronto, Toronto, Ontario, Canada. FAU - Ahmad, Fayeza AU - Ahmad F AD - Division of Cardiac Surgery, Jewish General Hospital, McGill University, Montreal, Quebec, Canada. FAU - Sarfaraz, Ziyab AU - Sarfaraz Z AD - Division of Cardiac Surgery, Houston Methodist, Houston, Tex. FAU - Hirsch, Andrew AU - Hirsch A AD - Division of Pulmonary Diseases, Department of Medicine, Jewish General Hospital, McGill University, Montreal, Quebec, Canada. AD - Center of Excellence in Thrombosis and Anticoagulation Care, Jewish General Hospital, McGill University, Montreal, Quebec, Canada. FAU - Morin, Jean-Francois AU - Morin JF AD - Division of Cardiac Surgery, Jewish General Hospital, McGill University, Montreal, Quebec, Canada. LA - eng PT - Journal Article DEP - 20260710 PL - Netherlands TA - JTCVS Open JT - JTCVS open JID - 101768541 PMC - PMC13711198 OTO - NOTNLM OT - chronic thromboembolic pulmonary hypertension OT - computed tomography pulmonary angiography OT - digital subtraction pulmonary angiography OT - pulmonary endarterectomy COIS- The authors reported no conflicts of interest. The Journal policy requires editors and reviewers to disclose conflicts of interest and to decline handling or reviewing manuscripts for which they may have a conflict of interest. The editors and reviewers of this article have no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/07/10 CRDT- 2026/10/28 05:32 PHST- 2026/01/22 00:00 [received] PHST- 2026/06/12 00:00 [revised] PHST- 2026/06/26 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:32 [entrez] PHST- 2026/07/10 00:00 [pmc-release] AID - S2666-2736(26)00383-9 [pii] AID - 101960 [pii] AID - 10.1016/j.xjon.2026.101960 [doi] PST - epublish SO - JTCVS Open. 2026 Jul 10;33:101960. doi: 10.1016/j.xjon.2026.101960. eCollection 2026 Oct. PMID- 42948421 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 3050-6328 (Electronic) IS - 3050-6328 (Linking) VI - 8 DP - 2026 Dec TI - Structural modeling predicts altered disulfide connectivity in HER2 W452C. PG - 100064 LID - 10.1016/j.premed.2026.100064 [doi] AB - HER2, an ERBB2-encoded receptor tyrosine kinase, regulates cell growth and survival. HER2 overexpression or ERBB2 amplification can promote tumor growth and is used to identify cancers that may benefit from HER2-targeted therapies. However, the functional consequences of sequence variants are difficult to predict when they occur outside known functional sites. Cysteine-introducing variants pose a challenge because a new cysteine can either form an abnormal bond directly or disrupt an existing disulfide and change which interactions are possible. HER2 W452C variant provides a case study of this problem because it introduces a cysteine outside the canonical dimerization interface but near a native extracellular disulfide. We used structural modeling, molecular dynamics, protein-protein docking, and cell-based analysis to test how W452C could alter receptor organization. Direct C452-C452 pairing between HER2 receptors was consistently difficult to accommodate without severe receptor overlap. We therefore examined a conditional state in which the native disulfide was reorganized, leaving another cysteine available for intermolecular interaction. This state increased ectodomain flexibility and accommodated alternative cysteine-mediated contacts. Explicit-bond refinement showed that selected C452-C504 arrangements remained structurally compatible after covalent disulfide formation. In cells, W452C increased a DTT-sensitive high-molecular-weight HER2-containing species, supporting altered disulfide-dependent association. Together, these findings support a mechanism in which an introduced cysteine alters receptor organization by reorganizing the local disulfide network rather than simply forming a new bond itself. More broadly, they illustrate how structural analysis can reveal molecular consequences of genetic variants that are not apparent from sequence position alone and thereby inform precision-medicine variant interpretation. FAU - Ngo, Khoa AU - Ngo K AUID- ORCID: 0000-0002-7454-2924 AD - Center for Precision Medicine and Data Sciences, University of California, Davis, School of Medicine, Sacramento, CA, USA. AD - Department of Physiology and Membrane Biology, University of California, Davis, School of Medicine, Davis, CA, USA. FAU - Saldana, Matthew AU - Saldana M AD - Department of Biochemistry and Molecular Medicine, University of California, Davis, School of Medicine, Sacramento, CA, USA. FAU - Amini, Hajar AU - Amini H AD - Center for Precision Medicine and Data Sciences, University of California, Davis, School of Medicine, Sacramento, CA, USA. AD - Department of Physiology and Membrane Biology, University of California, Davis, School of Medicine, Davis, CA, USA. FAU - Carraway, Kermit L AU - Carraway KL AD - Center for Precision Medicine and Data Sciences, University of California, Davis, School of Medicine, Sacramento, CA, USA. AD - Department of Biochemistry and Molecular Medicine, University of California, Davis, School of Medicine, Sacramento, CA, USA. FAU - Clancy, Colleen E AU - Clancy CE AUID- ORCID: 0000-0001-6849-4885 AD - Center for Precision Medicine and Data Sciences, University of California, Davis, School of Medicine, Sacramento, CA, USA. AD - Department of Physiology and Membrane Biology, University of California, Davis, School of Medicine, Davis, CA, USA. AD - Department of Pharmacology, University of California, Davis, School of Medicine, Davis, CA, USA. LA - eng GR - R01 HL128537/HL/NHLBI NIH HHS/United States GR - R01 HL174001/HL/NHLBI NIH HHS/United States PT - Journal Article DEP - 20261007 PL - England TA - J Precis Med (Amst) JT - Journal of precision medicine (Amsterdam, Netherlands) JID - 9919252216806676 PMC - PMC13712401 MID - NIHMS2216814 OTO - NOTNLM OT - Disulfide connectivity OT - ERBB2 OT - HER2 OT - Molecular dynamics OT - Receptor organization OT - Structural modeling OT - Variant interpretation OT - W452C EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/29 CRDT- 2026/10/28 05:31 PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:31 [entrez] PHST- 2026/10/29 00:00 [pmc-release] AID - 10.1016/j.premed.2026.100064 [doi] PST - ppublish SO - J Precis Med (Amst). 2026 Dec;8:100064. doi: 10.1016/j.premed.2026.100064. Epub 2026 Oct 7. PMID- 42948404 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1663-9812 (Print) IS - 1663-9812 (Electronic) IS - 1663-9812 (Linking) VI - 17 DP - 2026 TI - PANoptosis: a novel therapeutic target and mechanistic insight into traditional Chinese medicine intervention in inflammatory bowel disease: a review. PG - 1880406 LID - 10.3389/fphar.2026.1880406 [doi] LID - 1880406 AB - The rising incidence of inflammatory bowel disease (IBD) and the limited efficacy of current therapies underscore the need for novel strategies. PANoptosis is an inflammatory programmed cell-death process integrating molecular features of pyroptosis, apoptosis, and necroptosis. Emerging evidence suggests that PANoptosis-related genes and signaling nodes may contribute to intestinal epithelial injury, mucosal immune activation, barrier disruption, and inflammatory amplification in inflammatory bowel disease. Traditional Chinese medicine and its active constituents have been reported to regulate inflammation, intestinal barrier integrity, gut microbiota, and several cell-death pathways. However, direct evidence that these agents inhibit bona fide PANoptosis in intestinal models remains limited. Current mechanistic evidence is strongest for selected compounds, such as baicalin, which has been shown in macrophages to interfere with mitochondrial Z-DNA formation and ZBP1-PANoptosome assembly, whereas other proposed mechanisms are largely extrapolated from extra-intestinal models or from studies of apoptosis, pyroptosis, necroptosis, and inflammatory signaling. This narrative review summarizes the molecular basis of PANoptosis in IBD and critically evaluates the potential of traditional Chinese medicine-derived compounds and formulas to modulate upstream sensors, adaptor proteins, and downstream effectors of PANoptosis-related pathways. We emphasize that future studies should verify these mechanisms in intestinal epithelial cells, intestinal organoids, DSS/TNBS/IL-10-deficient colitis models, and human IBD tissues. CI - Copyright (c) 2026 Li, Guan, Yu, Xu and An. FAU - Li, Xiaojian AU - Li X AD - Liaoning University of Traditional Chinese Medicine, Shenyang, China. FAU - Guan, Wei AU - Guan W AD - Department of Hemorrhoids and Fistula I, The Third Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, China. FAU - Yu, Yongduo AU - Yu Y AD - Department of Proctology, The Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, China. FAU - Xu, Xinyao AU - Xu X AD - Liaoning University of Traditional Chinese Medicine, Shenyang, China. FAU - An, Shujian AU - An S AD - Liaoning University of Traditional Chinese Medicine, Shenyang, China. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Pharmacol JT - Frontiers in pharmacology JID - 101548923 PMC - PMC13711112 OTO - NOTNLM OT - PANoptosis OT - inflammatory bowel disease OT - intestinal barrier OT - programmed cell death OT - traditional Chinese medicine COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:31 PHST- 2026/05/14 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:31 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 1880406 [pii] AID - 10.3389/fphar.2026.1880406 [doi] PST - epublish SO - Front Pharmacol. 2026 Oct 13;17:1880406. doi: 10.3389/fphar.2026.1880406. eCollection 2026. PMID- 42948402 OWN - NLM STAT- Publisher LR - 20261029 IS - 1754-5692 (Print) IS - 1754-5706 (Electronic) IS - 1754-5692 (Linking) DP - 2026 Oct 27 TI - Methylcyclohexane-assisted polypropylene upcycling over PtRu/Al(2)O(3) via tandem dehydrogenation-hydrogenolysis. LID - 10.1039/d6ee05130h [doi] AB - Catalytic hydroconversion of polyolefin waste typically relies on the addition of high-pressure hydrogen, which greatly impacts both the process implementation and economic viability. Here we report polypropylene (PP) hydrogenolysis coupled to methylcyclohexane (MCH) dehydrogenation providing an external-H(2)-free tandem route to naphtha-range liquid hydrocarbons. The best performing catalysis system was 1 nm sized PtRu nanoparticles (1 wt% Pt/1 wt% Ru) on gamma-Al(2)O(3). At 300 degrees C, this catalyst could facilitate over 96 wt% PP conversion with more than 88 wt% as naphtha-range liquid hydrocarbon products. This performance was retained over 6 recycles. Spectroscopic characterisation indicates the formation of electronically coupled PtRu domains, which promote MCH dehydrogenation and moderate C-C bond cleavage. Solvent screening shows that hydrocarbon liquids with different structures lead to pronounced differences in PP conversion and product selectivity. Molecular dynamics simulations of PP in representative solvents provide a qualitative picture of how solvent-dependent chain mobility and chain extension may influence polymer accessibility to catalytic sites. Techno-economic analysis and life-cycle assessment suggest that, under favorable feedstock assumptions, renewable MCH could lower both carbon intensity and production cost relative to a direct H(2)-based route. These results identify MCH-assisted PtRu nanoparticle catalysis as a viable tandem process for PP upcycling and highlight the importance of coupling hydrogen-donor chemistry with liquid-phase transport effects in plastic waste valorisation. CI - This journal is (c) The Royal Society of Chemistry. FAU - Wang, Haokun AU - Wang H AUID- ORCID: 0009-0001-2075-6775 AD - Chemistry Research Laboratory, Department of Chemistry, University of Oxford 12 Mansfield Road Oxford OX1 3TA UK dermot.ohare@chem.ox.ac.uk. FAU - Zong, Zhiyuan AU - Zong Z AD - Department of Engineering Science, University of Oxford Parks Road Oxford OX1 3PJ UK. FAU - Luo, Mingyu AU - Luo M AD - Inorganic Chemistry Laboratory, University of Oxford South Parks Road Oxford OX1 3QR UK. FAU - Yang, Frederick Z T AU - Yang FZT AD - Chemistry Research Laboratory, Department of Chemistry, University of Oxford 12 Mansfield Road Oxford OX1 3TA UK dermot.ohare@chem.ox.ac.uk. FAU - Pan, Jiaying AU - Pan J AD - Department of Engineering Science, University of Oxford Parks Road Oxford OX1 3PJ UK. FAU - Wu, Zeyu AU - Wu Z AD - Inorganic Chemistry Laboratory, University of Oxford South Parks Road Oxford OX1 3QR UK. FAU - Liu, Rui AU - Liu R AD - Inorganic Chemistry Laboratory, University of Oxford South Parks Road Oxford OX1 3QR UK. FAU - Luo, Yuhong AU - Luo Y AD - Inorganic Chemistry Laboratory, University of Oxford South Parks Road Oxford OX1 3QR UK. FAU - Li, Jiasi AU - Li J AD - Inorganic Chemistry Laboratory, University of Oxford South Parks Road Oxford OX1 3QR UK. FAU - Li, Yiyang AU - Li Y AUID- ORCID: 0000-0002-9278-7386 AD - Inorganic Chemistry Laboratory, University of Oxford South Parks Road Oxford OX1 3QR UK. FAU - Turnell-Ritson, Roland C AU - Turnell-Ritson RC AUID- ORCID: 0000-0003-3702-9467 AD - Chemistry Research Laboratory, Department of Chemistry, University of Oxford 12 Mansfield Road Oxford OX1 3TA UK dermot.ohare@chem.ox.ac.uk. FAU - Stamatakis, Michail AU - Stamatakis M AUID- ORCID: 0000-0001-8338-8706 AD - Inorganic Chemistry Laboratory, University of Oxford South Parks Road Oxford OX1 3QR UK. FAU - Yang, Aidong AU - Yang A AD - Department of Engineering Science, University of Oxford Parks Road Oxford OX1 3PJ UK. FAU - O' Hare, Dermot AU - O' Hare D AUID- ORCID: 0000-0001-8054-8751 AD - Chemistry Research Laboratory, Department of Chemistry, University of Oxford 12 Mansfield Road Oxford OX1 3TA UK dermot.ohare@chem.ox.ac.uk. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Energy Environ Sci JT - Energy & environmental science JID - 101513897 PMC - PMC13711158 COIS- There are no conflicts to declare. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/27 CRDT- 2026/10/28 05:30 PHST- 2026/07/29 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:30 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - d6ee05130h [pii] AID - 10.1039/d6ee05130h [doi] PST - aheadofprint SO - Energy Environ Sci. 2026 Oct 27. doi: 10.1039/d6ee05130h. PMID- 42948393 OWN - NLM STAT- Publisher LR - 20261029 IS - 2046-2069 (Electronic) IS - 2046-2069 (Linking) DP - 2026 Oct 27 TI - Surfactant-free electrochemical synthesis of layered Ag/Au bimetallic nanostructures as SERS platforms with DFT insights into their interfacial charge transfer mechanism. LID - 10.1039/d6ra08211d [doi] AB - Bimetallic nanoparticles have attracted significant interest in Surface-Enhanced Raman Scattering (SERS) due to their superior optical and synergistic properties. In this study, layered Ag@Au bimetallic nanostructures were fabricated on Indium Tin Oxide (ITO) glass via a surfactant-free electrochemical approach combining linear sweep voltammetry (LSV) and chronoamperometry (CA). This method rapidly yields clean, highly active plasmonic interfaces, effectively eliminating the competitive adsorption typically caused by chemical stabilisers. By featuring a rugged morphology and an exposed bimetallic interface, the optimal substrate generates abundant electromagnetic hot spots to deliver superior SERS sensitivity for Nile Blue A (NBA). Interestingly, the empirical spectra exhibited a highly selective, vibrational-mode-dependent signal amplification, indicating a restricted, perpendicular adsorption orientation of the NBA molecules within the bimetallic nanogaps. To fundamentally elucidate the localised Chemical Enhancement (CE) mechanism driving this selective behaviour, Density Functional Theory (DFT) calculations were performed on representative pyramidal clusters. Consistent with the stoichiometry of the superior experimental substrate, the computational models demonstrated that the bimetallic Ag(16)Au(4) cluster yields the strongest SERS enhancement. Charge density difference (CDD) maps and natural population analysis (NPA) showed that the largest electron transfer from the NBA ligand to the Ag(16)Au(4) cluster occurred through the interaction between the N atom of the oxazine ring and the Au atom at the top of the cluster, resulting in the greatest enhancement. Hence, this synergistic experimental-theoretical approach paves the way for the design of bimetallic sensors for advanced environmental analysis. CI - This journal is (c) The Royal Society of Chemistry. FAU - Ngo, Minh Nhat AU - Ngo MN AD - School of Chemistry and Life Sciences, Hanoi University of Science and Technology 1 Dai Co Viet Hanoi Vietnam. FAU - Thi-Thu Le, Hang AU - Thi-Thu Le H AUID- ORCID: 0000-0003-4230-9498 AD - School of Chemistry and Life Sciences, Hanoi University of Science and Technology 1 Dai Co Viet Hanoi Vietnam. FAU - Ngo, Thi Chinh AU - Ngo TC AUID- ORCID: 0000-0002-8206-5996 AD - Institute of Research and Development, Duy Tan University Da Nang 550000 Vietnam. AD - School of Engineering and Technology, Duy Tan University Da Nang 550000 Vietnam mai.nguyenthituyet1@hust.edu.vn ngothichinh@duytan.edu.vn. FAU - Thi-Tuyet Nguyen, Mai AU - Thi-Tuyet Nguyen M AUID- ORCID: 0000-0002-8901-2395 AD - School of Chemistry and Life Sciences, Hanoi University of Science and Technology 1 Dai Co Viet Hanoi Vietnam. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - RSC Adv JT - RSC advances JID - 101581657 PMC - PMC13711122 COIS- The authors declare no competing interests. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/27 CRDT- 2026/10/28 05:30 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:30 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - d6ra08211d [pii] AID - 10.1039/d6ra08211d [doi] PST - aheadofprint SO - RSC Adv. 2026 Oct 27. doi: 10.1039/d6ra08211d. PMID- 42948391 OWN - NLM STAT- Publisher LR - 20261029 IS - 2046-2069 (Electronic) IS - 2046-2069 (Linking) DP - 2026 Oct 27 TI - Solubility parameters of enzymatic hydrolysis lignin: a comparative study of inverse gas chromatography and Hansen solubility sphere. LID - 10.1039/d6ra09398a [doi] AB - Enzymatic hydrolysis lignin (EHL), a major byproduct of second-generation bioethanol production, is a promising renewable feedstock but remains challenging, in part because systematic solvent screening for EHL processing is largely absent from the literature. In this work, we combine inverse gas chromatography (IGC) with gravimetric solubility measurements to establish a practical, experimentally validated framework for solvent selection in EHL processing. Infinite dilution activity coefficients were measured for 31 probe solvents across 408.15-438.15 K. The IGC data were used to determine Hansen solubility parameters (HSPs): delta (D) = 19.15, delta (P) = 13.01, delta (H) = 8.30 and delta (t) = 24.58 MPa(0.5) while the interaction radius R (0) = 8.98 MPa(0.5) was fitted using the gravimetric solubility experiments. Surface properties were also evaluated using the Snyder-Karger model. Polar and hydrogen-bonding interactions were identified as the dominant drivers of EHL-solvent affinity. The resulting HSP solubility sphere was validated against gravimetric solubility measurements for 22 solvents, where bio-based solvents such as gamma-valerolactone was located within the EHL solubility sphere, highlighting their potential as promising bio-based solvent candidates for further evaluation in lignin processing. This work supports the development of an experimental basis for selecting alternative solvents for EHL processing and contributes to more sustainable routes for lignin valorization in lignocellulosic biorefineries. CI - This journal is (c) The Royal Society of Chemistry. FAU - Ossama, Muhammad AU - Ossama M AUID- ORCID: 0009-0002-4363-1913 AD - Aalto University, School of Chemical Engineering, Department of Chemical and Metallurgical Engineering, Research Group of Chemical Engineering P.O. Box 16100 FI-00076 Aalto Finland Muhammad.ossama@aalto.fi. FAU - Laakso, Juho-Pekka AU - Laakso JP AUID- ORCID: 0009-0005-0993-7013 AD - Aalto University, School of Chemical Engineering, Department of Chemical and Metallurgical Engineering, Research Group of Chemical Engineering P.O. Box 16100 FI-00076 Aalto Finland Muhammad.ossama@aalto.fi. FAU - Luci, Robin AU - Luci R AD - Aalto University, School of Chemical Engineering, Department of Chemical and Metallurgical Engineering, Research Group of Chemical Engineering P.O. Box 16100 FI-00076 Aalto Finland Muhammad.ossama@aalto.fi. FAU - Wibowo, Agung Ari AU - Wibowo AA AUID- ORCID: 0000-0001-7747-9417 AD - Aalto University, School of Chemical Engineering, Department of Chemical and Metallurgical Engineering, Research Group of Chemical Engineering P.O. Box 16100 FI-00076 Aalto Finland Muhammad.ossama@aalto.fi. FAU - Asadi, Ehsan AU - Asadi E AUID- ORCID: 0000-0001-9662-6566 AD - Aalto University, School of Chemical Engineering, Department of Chemical and Metallurgical Engineering, Research Group of Chemical Engineering P.O. Box 16100 FI-00076 Aalto Finland Muhammad.ossama@aalto.fi. FAU - Uusi-Kyyny, Petri AU - Uusi-Kyyny P AUID- ORCID: 0000-0002-8339-4601 AD - Aalto University, School of Chemical Engineering, Department of Chemical and Metallurgical Engineering, Research Group of Chemical Engineering P.O. Box 16100 FI-00076 Aalto Finland Muhammad.ossama@aalto.fi. FAU - Alopaeus, Ville AU - Alopaeus V AUID- ORCID: 0000-0001-9773-0998 AD - Aalto University, School of Chemical Engineering, Department of Chemical and Metallurgical Engineering, Research Group of Chemical Engineering P.O. Box 16100 FI-00076 Aalto Finland Muhammad.ossama@aalto.fi. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - RSC Adv JT - RSC advances JID - 101581657 PMC - PMC13711123 COIS- The authors declare that they have no competing interests. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/27 CRDT- 2026/10/28 05:30 PHST- 2026/09/28 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:30 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - d6ra09398a [pii] AID - 10.1039/d6ra09398a [doi] PST - aheadofprint SO - RSC Adv. 2026 Oct 27. doi: 10.1039/d6ra09398a. PMID- 42948387 OWN - NLM STAT- Publisher LR - 20261029 IS - 2046-2069 (Electronic) IS - 2046-2069 (Linking) DP - 2026 Oct 27 TI - A novel foam-board-based plasmonic chemosensor towards specific recognition of formaldehyde and acetaldehyde using silver nanoprism reinforced by 1,10-phenanthroline and d-penicillamine: construction of a miniaturized optical device by 3D printer towards rapid pharmaceutical analysis. LID - 10.1039/d6ra07451k [doi] AB - Formaldehyde (FA) and acetaldehyde (AA) are highly reactive aldehydes that widely distributed in environmental matrices. Due to their potential toxicity and adverse health effects, the development of sensitive, selective, methods for their determination are of significant importance. But, quantification of FA and AA by conventional method require expensive instruments, sample preparation steps, export operators which load to limitation of its application for on-site analysis of volatile organic compounds (VOCs). In this study, a novel plasmonic colorimetric sensing device based on foam-board decorated by silver nanoprisms (AgNPrs) reinforced by with 1,10 phenanthroline (OFA) and d-penicillamine (DPA) was developed for the selective detection of FA and AA. The sensing mechanism relies on analyte-induced modulation of the localized surface plasmon resonance (LSPR) characteristics of AgNPrs, resulting in distinct colorimetric responses that can be visually observed and quantitatively analyzed using smartphone-assisted digital image processing. Quantitative analysis was performed using RGB histogram analysis, providing reliable FA and AA sensor in low volume of analyte and optional probe without the need for laboratory instrumentation. Under optimized conditions, the proposed photo-sensing platform exhibited a dynamic linear detection range of 1 to 200 microM for both FA and AA. The analytical capability of the developed sensing strategy was further validated in human urine samples, demonstrating reliable performance in complex biological matrices. The integration of plasmonic AgNPrs, selective ligand (DPA and OFA), smartphone-assisted image, and a flexible miniaturized sensing substrate provides a simple, low-cost, and portable colorimetric chemosensor for aldehyde monitoring in real samples. The proposed chemosensor shows a novel strategy for on-site recognition of VOCs. CI - This journal is (c) The Royal Society of Chemistry. FAU - Bahavarnia, Farnaz AU - Bahavarnia F AD - Nutrition Research Center, Tabriz University of Medical Sciences Tabriz Iran. FAU - Hasanzadeh, Mohammad AU - Hasanzadeh M AUID- ORCID: 0000-0003-4918-1239 AD - Pharmaceutical Analysis Research Center, Pharmaceutical Science Institute, Tabriz University of Medical Sciences Tabriz Iran hasanzadehm@tbzmed.ac.ir. FAU - Shadjou, Nasrin AU - Shadjou N AD - Department of Nanotechnology, Faculty of Chemistry, Urmia University Urmia Iran. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - RSC Adv JT - RSC advances JID - 101581657 PMC - PMC13711126 COIS- There are no conflicts to declare. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/27 CRDT- 2026/10/28 05:30 PHST- 2026/08/11 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:30 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - d6ra07451k [pii] AID - 10.1039/d6ra07451k [doi] PST - aheadofprint SO - RSC Adv. 2026 Oct 27. doi: 10.1039/d6ra07451k. PMID- 42948386 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2211-3835 (Print) IS - 2211-3843 (Electronic) IS - 2211-3835 (Linking) VI - 16 IP - 10 DP - 2026 Oct TI - A mechanistic quantitative systems pharmacology framework enriched by multiscale data enables model-informed translational development of mesenchymal stem cell therapy in lupus nephritis. PG - 6756-6779 LID - 10.1016/j.apsb.2026.08.018 [doi] AB - Lupus nephritis (LN) is a complex autoimmune disease and mesenchymal stem cells (MSCs), given their unique immune-modulatory potential, have emerged as a promising route for treating LN. Understanding the multiscale dynamic LN-MSC interactions and selecting the optimal clinical design for MSCs is critical to enhance their therapeutic potential and achieve meaningful benefit-risk differentiation in patients. To guide MSC clinical development, we constructed the first mechanistic quantitative systems pharmacology model of LN pathophysiology that integrated multimodal preclinical-clinical data, with detailed illustration of standard-of-care immunosuppressant pharmacology and physiologically-based biodistribution and therapeutic mechanisms of MSCs. We created model-based virtual LN patients that well captured clinical interpatient heterogeneity and were validated by numerous sets of clinical efficacy readouts, including first-hand results from our investigator-initiated trial testing MSCs in LN patients. We used this computational framework and identified that a spaced two-infusion MSC dosing strategy would yield optimal clinical response in LN patients, which directly informed the phase 2 design of a novel MSC therapy. Modeling analyses further identified that CXCL10 in combination with proteinuria are potential predictive biomarkers that could boost clinical renal response to MSCs. These results demonstrated the exceptional importance of our QSP framework in driving MSC clinical development for LN patients. CI - (c) 2026 The Authors. FAU - Zhang, Sujie AU - Zhang S AD - School of Pharmacy, Nanjing Medical University, Nanjing 210000, China. AD - School of Medicine, Yangzhou Polytechnic University, Yangzhou 225000, China. FAU - Zhang, Qixiang AU - Zhang Q AD - Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210000, China. FAU - Wang, Dandan AU - Wang D AD - Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210000, China. FAU - Li, Zichun AU - Li Z AD - School of Pharmacy, Nanjing Medical University, Nanjing 210000, China. FAU - Xiao, Xia AU - Xiao X AD - School of Pharmacy, Nanjing Medical University, Nanjing 210000, China. FAU - Rao, Qi AU - Rao Q AD - School of Pharmacy, Nanjing Medical University, Nanjing 210000, China. FAU - Yang, Siyuan AU - Yang S AD - School of Pharmacy, Nanjing Medical University, Nanjing 210000, China. FAU - Qu, Yuchen AU - Qu Y AD - School of Pharmacy, Nanjing Medical University, Nanjing 210000, China. FAU - Xu, Yiyang AU - Xu Y AD - School of Pharmacy, Nanjing Medical University, Nanjing 210000, China. FAU - Wei, Ning AU - Wei N AD - Jiangsu Renocell Biotech Co., Ltd., Nanjing 210000, China. FAU - Wang, Jing AU - Wang J AD - Jiangsu Renocell Biotech Co., Ltd., Nanjing 210000, China. FAU - Gao, Yuan AU - Gao Y AD - QSPMed Technologies, Nanjing 210000, China. FAU - Sun, Lingyun AU - Sun L AD - Department of Rheumatology and Immunology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing 210000, China. FAU - Wang, Guangji AU - Wang G AD - Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210000, China. FAU - Zhou, Fang AU - Zhou F AD - Key Laboratory of Drug Metabolism and Pharmacokinetics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210000, China. FAU - Zhao, Chen AU - Zhao C AD - School of Pharmacy, Nanjing Medical University, Nanjing 210000, China. AD - Department of Pharmacy, the First Affiliated Hospital with Nanjing Medical University, Nanjing 210000, China. LA - eng PT - Journal Article DEP - 20260825 PL - Netherlands TA - Acta Pharm Sin B JT - Acta pharmaceutica Sinica. B JID - 101600560 PMC - PMC13712365 OTO - NOTNLM OT - Clinical translation OT - Clinical trial simulation OT - Lupus nephritis OT - Mesenchymal stem cell OT - Model-informed drug development OT - Patient stratification OT - Quantitative systems pharmacology OT - Virtual patients COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/08/25 CRDT- 2026/10/28 05:30 PHST- 2025/07/19 00:00 [received] PHST- 2026/01/11 00:00 [revised] PHST- 2026/02/27 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:30 [entrez] PHST- 2026/08/25 00:00 [pmc-release] AID - S2211-3835(26)00548-4 [pii] AID - 10.1016/j.apsb.2026.08.018 [doi] PST - ppublish SO - Acta Pharm Sin B. 2026 Oct;16(10):6756-6779. doi: 10.1016/j.apsb.2026.08.018. Epub 2026 Aug 25. PMID- 42948381 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1178-7031 (Print) IS - 1178-7031 (Electronic) IS - 1178-7031 (Linking) VI - 19 DP - 2026 TI - Machine Learning-Based Diagnosis of Coronary Heart Disease with Clinical and Biomarker Data. PG - 615123 LID - 10.2147/JIR.S615123 [doi] LID - 615123 AB - PURPOSE: Coronary heart disease (CHD) is a common condition associated with high disability and mortality. Although coronary angiography (CAG) remains the gold standard for diagnosis, its accessibility is limited in medically underserved regions or for patients who are ineligible for the procedure. The aim of this study was to develop a prediction model using machine learning algorithms that integrate clinical data and plasma biomarkers for timely and noninvasive CHD diagnosis. PATIENTS AND METHODS: A total of 191 patients who underwent CAG at the First Affiliated Hospital of Henan University between March and October 2023, comprising 151 CHD patients and 40 non-CHD controls, were included. Clinical data and plasma samples were collected; the levels of eight biomarkers (LPA, MCP-1, TNF-alpha, IL-1beta, NRG4, S100A8/A9, HMGB1, and cfDNA) were measured by ELISAs and fluorescence spectrophotometry. The dataset was split into training, test, and validation sets (8:1:1) and standardized. After the SMOTE was applied to the training set, key features were selected via recursive feature elimination with cross-validation (RFECV). Six machine learning algorithms were used to construct the models, and their performance was evaluated on the test set and via 10-fold cross-validation. SHAP was used to interpret the optimal model. RESULTS: Eight important features were identified. Among the six models, the KNN model performed best on the test set, achieving an AUROC of 80.7%, accuracy of 82.7%, F1‑score of 89.5%, precision of 86.4%, and sensitivity of 92.7%. CONCLUSION: A machine learning model integrating clinical and plasma biomarker data has potential for predicting CHD in patients who present with chest discomfort, offering a potential diagnostic aid when CAG is not readily accessible, pending validation in larger, multicenter studies. However, these findings are based on a single-center cohort of 191 patients and require external validation in larger, multicenter populations. CI - (c) 2026 Zhang et al. FAU - Zhang, Zhengwei AU - Zhang Z AUID- ORCID: 0000-0002-8967-1972 AD - Department of Cardiology, the First Affiliated Hospital, Henan University, Kaifeng, Henan, 475000, People's Republic of China. AD - Kaifeng Key Laboratory for Modulation and Rehabilitation of Cardiac Function, Kaifeng, Henan, 475000, People's Republic of China. FAU - Zhao, Ang AU - Zhao A AUID- ORCID: 0009-0001-5195-3296 AD - Department of Cardiology, the First Affiliated Hospital, Henan University, Kaifeng, Henan, 475000, People's Republic of China. AD - Kaifeng Key Laboratory for Modulation and Rehabilitation of Cardiac Function, Kaifeng, Henan, 475000, People's Republic of China. AD - Department of Cardiology, Zhoukou Central Hospital, Zhoukou Medical Science Research Center, Zhoukou, Henan, 466000, People's Republic of China. FAU - Mi, Yutian AU - Mi Y AD - Department of Cardiology, the First Affiliated Hospital, Henan University, Kaifeng, Henan, 475000, People's Republic of China. AD - Kaifeng Key Laboratory for Modulation and Rehabilitation of Cardiac Function, Kaifeng, Henan, 475000, People's Republic of China. FAU - Qiao, Yu AU - Qiao Y AUID- ORCID: 0009-0002-3965-7774 AD - Department of Cardiology, the First Affiliated Hospital, Henan University, Kaifeng, Henan, 475000, People's Republic of China. AD - Kaifeng Key Laboratory for Modulation and Rehabilitation of Cardiac Function, Kaifeng, Henan, 475000, People's Republic of China. FAU - Xue, Shuangyang AU - Xue S AD - Department of Cardiology, the First Affiliated Hospital, Henan University, Kaifeng, Henan, 475000, People's Republic of China. AD - Kaifeng Key Laboratory for Modulation and Rehabilitation of Cardiac Function, Kaifeng, Henan, 475000, People's Republic of China. FAU - Zhang, Fuwei AU - Zhang F AD - Department of Cardiology, the First Affiliated Hospital, Henan University, Kaifeng, Henan, 475000, People's Republic of China. AD - Kaifeng Key Laboratory for Modulation and Rehabilitation of Cardiac Function, Kaifeng, Henan, 475000, People's Republic of China. FAU - Yang, Jingmin AU - Yang J AD - Department of Cardiology, the First Affiliated Hospital, Henan University, Kaifeng, Henan, 475000, People's Republic of China. AD - Kaifeng Key Laboratory for Modulation and Rehabilitation of Cardiac Function, Kaifeng, Henan, 475000, People's Republic of China. FAU - Song, Zhiming AU - Song Z AD - Department of Cardiology, the First Affiliated Hospital, Henan University, Kaifeng, Henan, 475000, People's Republic of China. AD - Kaifeng Key Laboratory for Modulation and Rehabilitation of Cardiac Function, Kaifeng, Henan, 475000, People's Republic of China. AD - Institute on Aging and Disease, Henan University, Kaifeng, Henan, 475000, People's Republic of China. LA - eng PT - Journal Article DEP - 20261023 PL - New Zealand TA - J Inflamm Res JT - Journal of inflammation research JID - 101512684 PMC - PMC13712231 OTO - NOTNLM OT - biomarkers OT - coronary heart disease OT - machine learning COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/23 CRDT- 2026/10/28 05:30 PHST- 2026/05/20 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:30 [entrez] PHST- 2026/10/23 00:00 [pmc-release] AID - 615123 [pii] AID - 10.2147/JIR.S615123 [doi] PST - epublish SO - J Inflamm Res. 2026 Oct 23;19:615123. doi: 10.2147/JIR.S615123. eCollection 2026. PMID- 42948380 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2578-9430 (Electronic) IS - 2578-9430 (Linking) VI - 2026 DP - 2026 TI - High salinity selects for halotolerant gut microbiota in the mummichog Fundulus heteroclitus. LID - 10.17912/micropub.biology.002267 [doi] AB - Mummichogs ( Fundulus heteroclitus ) are euryhaline fish that maintain osmoregulation across a range of salinities, but how their gut microbiomes respond remains poorly understood. Using Illumina 16S amplicon and Oxford Nanopore full-length 16S sequencing, we show that salinity is associated with reproducible gut microbiome restructuring. While alpha diversity did not differ significantly between low-salinity (5 ppt) and high-salinity (50 ppt) groups, beta diversity revealed distinct shifts in microbial communities. Specifically, high-salinity microbiomes were dominated by halotolerant Vibrio and Ahrensia species while low-salinity microbiomes retained more taxonomically diverse communities. These findings suggest host-associated microbes may contribute to osmotic homeostasis under salinity stress. CI - Copyright: (c) 2026 by the authors. FAU - Bradberry, Reece AU - Bradberry R AD - Department of Biology, Presbyterian College, Clinton, SC, United States. FAU - Hutchinson, Elyse AU - Hutchinson E AD - Department of Biology, Presbyterian College, Clinton, SC, United States. FAU - Edwards, Carlie AU - Edwards C AD - Department of Biology, Presbyterian College, Clinton, SC, United States. FAU - Wetzel, James AU - Wetzel J AD - Department of Biology, Presbyterian College, Clinton, SC, United States. FAU - Shull, Austin Y AU - Shull AY AD - Department of Biology, Presbyterian College, Clinton, SC, United States. FAU - Gordon, Stuart AU - Gordon S AD - Department of Biology, Presbyterian College, Clinton, SC, United States. LA - eng PT - Journal Article DEP - 20261012 PL - United States TA - MicroPubl Biol JT - microPublication biology JID - 101759238 PMC - PMC13711128 COIS- The authors declare that there are no conflicts of interest present. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/12 CRDT- 2026/10/28 05:29 PHST- 2026/07/01 00:00 [received] PHST- 2026/08/14 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:29 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - 10.17912/micropub.biology.002267 [doi] PST - epublish SO - MicroPubl Biol. 2026 Oct 12;2026:10.17912/micropub.biology.002267. doi: 10.17912/micropub.biology.002267. eCollection 2026. PMID- 42948379 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1792-1082 (Electronic) IS - 1792-1074 (Print) IS - 1792-1074 (Linking) VI - 32 IP - 6 DP - 2026 Dec TI - An interpretable machine-learning model to predict moderate-to-severe acute radiation esophagitis following regional nodal irradiation for breast cancer. PG - 555 LID - 10.3892/ol.2026.15909 [doi] LID - 555 AB - Acute radiation esophagitis (ARE) is a clinically relevant toxicity occurring in patients with breast cancer receiving post-operative regional nodal irradiation. The present study aimed to develop and evaluate an interpretable machine-learning (ML) model for grade >/=2 ARE. A total of 324 patients were allocated by outcome-stratified random sampling (7:3) to a training set (n=228) and a held-out test set (n=96). A total of six consensus predictors were retained: Pre-existing pruritus, histological grade, body mass index (BMI), diabetes, tumor laterality and the relative esophageal volume receiving >/=30 Gy (RV_V30). In total, 10 ML algorithms were evaluated. Bootstrap internal validation (1,000 resamples) revealed that logistic regression (LR) had the lowest mean optimism among the three preferred candidates (0.027). Final model selection considered bootstrap stability and corrected performance together with held-out discrimination and calibration, model complexity and clinical interpretability. In the held-out test set, LR achieved an area under the curve value of 0.797 (95% confidence interval, 0.706-0.889), a Brier score of 0.179, a calibration slope of 1.258 and a calibration-in-the-large of -0.056. Decision curve analysis revealed positive net benefit relative to treat-all and treat-none strategies across threshold probabilities of 10-66%. SHapley Additive exPlanations identified histological grade, RV_V30 and BMI as the most influential predictors. The final model was implemented as a nomogram and web-based calculator. Overall, the present study demonstrates that this six-feature clinical-dosimetric model may support individualized risk stratification and radiotherapy planning, but prospective multicenter external validation is required before routine clinical use. CI - Copyright: (c) 2026 He et al. FAU - He, Bin AU - He B AD - Department of Oncology and Radiotherapy, Chengdu Qingbaijiang District People's Hospital, Chengdu, Sichuan 610300, P.R. China. AD - Department of Oncology, The General Hospital of Western Theater Command of The Chinese People's Liberation Army, Chengdu, Sichuan 610083, P.R. China. FAU - Qi, Zhongchun AU - Qi Z AD - Department of Oncology, The General Hospital of Western Theater Command of The Chinese People's Liberation Army, Chengdu, Sichuan 610083, P.R. China. FAU - Zhang, Hengdi AU - Zhang H AD - Department of Ophthalmology, The General Hospital of Western Theater Command of The Chinese People's Liberation Army, Chengdu, Sichuan 610083, P.R. China. FAU - Li, Linling AU - Li L AD - School of Clinical Medicine, Chengdu Medical College, Chengdu, Sichuan 610500, P.R. China. FAU - Li, Hua AU - Li H AD - Department of Oncology, The General Hospital of Western Theater Command of The Chinese People's Liberation Army, Chengdu, Sichuan 610083, P.R. China. FAU - Zou, Xinyun AU - Zou X AD - Department of Oncology, The General Hospital of Western Theater Command of The Chinese People's Liberation Army, Chengdu, Sichuan 610083, P.R. China. FAU - Tan, Yu AU - Tan Y AD - School of Clinical Medicine, Chengdu Medical College, Chengdu, Sichuan 610500, P.R. China. FAU - Zhang, Ling AU - Zhang L AD - Department of Oncology, The General Hospital of Western Theater Command of The Chinese People's Liberation Army, Chengdu, Sichuan 610083, P.R. China. FAU - Kong, Fangyuan AU - Kong F AD - Department of Oncology, The General Hospital of Western Theater Command of The Chinese People's Liberation Army, Chengdu, Sichuan 610083, P.R. China. LA - eng PT - Journal Article DEP - 20261016 PL - Greece TA - Oncol Lett JT - Oncology letters JID - 101531236 PMC - PMC13711150 OTO - NOTNLM OT - breast cancer OT - machine-learning OT - predictive model OT - radiation-induced esophagitis OT - regional lymph node radiotherapy COIS- The authors declare that they have no competing interests. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/16 CRDT- 2026/10/28 05:29 PHST- 2026/01/27 00:00 [received] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:29 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - OL-32-6-15909 [pii] AID - 10.3892/ol.2026.15909 [doi] PST - epublish SO - Oncol Lett. 2026 Oct 16;32(6):555. doi: 10.3892/ol.2026.15909. eCollection 2026 Dec. PMID- 42948375 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1792-1015 (Electronic) IS - 1792-0981 (Print) IS - 1792-0981 (Linking) VI - 32 IP - 6 DP - 2026 Dec TI - Targeted inhibition of Mycobacterium tuberculosis invasion into macrophages by Rv1768-Rv1983-loaded mannosylated chitosan nanoparticles. PG - 342 LID - 10.3892/etm.2026.13337 [doi] LID - 342 AB - Tuberculosis (TB) remains a major threat to global health. The protective efficacy of the Bacillus Calmette-Guerin vaccine is limited because of the absence of multiple virulence-related genomic regions in Mycobacterium tuberculosis (MTB). The surface proteins of MTB are closely associated with bacterial invasiveness and pathogenicity, representing promising targets for novel vaccine development. In the present study, the MTB polymorphic GC-rich sequence proteins (Pro-Glu and Pro-Pro-Glu-Pro-Arg-Gly-Ser) family fusion protein Rv1768-Rv1983 was cloned, expressed and encapsulated into mannosylated chitosan (MCS) to construct MCS-Rv1768Rv1983 nanoparticles. The immunological and protective effects of this nanoformulation were evaluated in vitro and in a mouse model. Physicochemical characterization revealed that the nanoparticles had a mean particle size of 196.3 nm and a drug loading efficiency of 8.02%. In vitro cell experiments demonstrated that MCS-Rv1768-Rv1983 exhibited no obvious cytotoxicity and stimulated CD4(+) T cells to secrete IFN-gamma and IL-4, as well as CD8(+) T cells, to produce IFN-gamma. Animal experiments revealed that immunization with MCS-Rv1768-Rv1983 markedly elevated serum IgG1 and IgG2a titres and that the induced antibodies significantly reduced the invasion of virulent MTB H37Rv into macrophages. After H37Rv challenge, compared with the control group, the MCS-Rv1768-Rv1983 immunization group exhibited significantly lower bacterial loads in mouse lungs and markedly longer survival times. These results confirm that MCS nanoparticles can increase the immune efficacy of the Rv1768-Rv1983 fusion protein, block MTB invasion into macrophages and may contribute to the early clearance of MTB by macrophages. CI - Copyright: (c) 2026 Zhou et al. FAU - Zhou, Jing AU - Zhou J AD - Wuhan Pulmonary Hospital (Wuhan Institute for Tuberculosis Control), Hubei Branch (Wuhan Pulmonary Hospital) of the National Clinical Research Center for Infectious Diseases, Wuhan, Hubei 430030, P.R. China. FAU - An, Mingwei AU - An M AD - State Key Laboratory of Biocatalysis and Enzyme Engineering, Department of Biochemistry and Molecular Biology, Life Science School of Hubei University, Wuhan, Hubei 430062, P.R. China. FAU - Zhao, Shidong AU - Zhao S AD - State Key Laboratory of Biocatalysis and Enzyme Engineering, Department of Biochemistry and Molecular Biology, Life Science School of Hubei University, Wuhan, Hubei 430062, P.R. China. FAU - Shan, Yuwei AU - Shan Y AD - State Key Laboratory of Biocatalysis and Enzyme Engineering, Department of Biochemistry and Molecular Biology, Life Science School of Hubei University, Wuhan, Hubei 430062, P.R. China. FAU - Yu, Zhoubing AU - Yu Z AD - Wuhan Biocause Pharmaceutical Development Co., Ltd., Wuhan, Hubei 430056, P.R. China. FAU - Xue, Yun AU - Xue Y AD - Laboratory of Medical Engineering, College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, Henan 471003, P.R. China. FAU - Chen, Fan AU - Chen F AD - State Key Laboratory of Biocatalysis and Enzyme Engineering, Department of Biochemistry and Molecular Biology, Life Science School of Hubei University, Wuhan, Hubei 430062, P.R. China. LA - eng PT - Journal Article DEP - 20261015 PL - Greece TA - Exp Ther Med JT - Experimental and therapeutic medicine JID - 101531947 PMC - PMC13711149 OTO - NOTNLM OT - Mycobacterium tuberculosis OT - Rv1768-Rv1983 fusion protein OT - macrophage invasion OT - mannosylated chitosan OT - nanoparticles COIS- The authors declare that they have no competing interests. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/15 CRDT- 2026/10/28 05:29 PHST- 2026/04/18 00:00 [received] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:29 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ETM-32-6-13337 [pii] AID - 10.3892/etm.2026.13337 [doi] PST - epublish SO - Exp Ther Med. 2026 Oct 15;32(6):342. doi: 10.3892/etm.2026.13337. eCollection 2026 Dec. PMID- 42948373 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 17 DP - 2026 TI - Targeting inhibitory phagocytosis checkpoints in cancer immunotherapy: mechanisms, therapeutic strategies, and translational challenges. PG - 1924227 LID - 10.3389/fimmu.2026.1924227 [doi] LID - 1924227 AB - Tumors evade macrophage-mediated clearance through mechanistically distinct but functionally convergent anti-phagocytic pathways. This review examines canonical and emerging receptor-ligand "don't eat me" axes, Siglec-dependent glyco-inhibitory checkpoints, and an emerging noncanonical glycocalyx barrier associated with sialylated CD43, linking these mechanisms to impaired macrophage recognition and activation, phagocytic synapse formation, and target-cell engulfment. We discuss their molecular basis, roles in tumor immune evasion, and therapeutic targeting, with emphasis on checkpoint-blocking antibodies, Fc-engineered antibodies, bispecific molecules, fusion proteins, and glyco-immune interventions. Clinical translation has nevertheless been uneven because of on-target/off-tumor toxicity, insufficient tumor selectivity, insufficient pro-phagocytic signaling, macrophage heterogeneity, and parallel immunosuppressive pathways. The strength and maturity of the supporting evidence vary considerably across targets, with CD47-SIRPalpha remaining the most extensively studied and clinically developed axis. Further progress will require mechanism- and evidence-guided target prioritization, biomarker-informed patient selection, and drug engineering to improve therapeutic selectivity and therapeutic windows; the clinical value of multi-checkpoint and other combination strategies remains to be established. CI - Copyright (c) 2026 Li, Ding, Sun, Gao, Zhou, Zeng, Xu, Li, Du, Huang and Chen. FAU - Li, Jie AU - Li J AD - Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China. AD - Guizhou Children's Hospital, Zunyi, Guizhou, China. FAU - Ding, Mei AU - Ding M AD - Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China. AD - Guizhou Children's Hospital, Zunyi, Guizhou, China. FAU - Sun, Jian AU - Sun J AD - Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China. AD - Guizhou Children's Hospital, Zunyi, Guizhou, China. FAU - Gao, Lu AU - Gao L AD - Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China. AD - Guizhou Children's Hospital, Zunyi, Guizhou, China. FAU - Zhou, Zhencang AU - Zhou Z AD - Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China. AD - Guizhou Children's Hospital, Zunyi, Guizhou, China. FAU - Zeng, Qian AU - Zeng Q AD - Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China. AD - Guizhou Children's Hospital, Zunyi, Guizhou, China. FAU - Xu, Fang AU - Xu F AD - Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China. AD - Guizhou Children's Hospital, Zunyi, Guizhou, China. FAU - Li, Siming AU - Li S AD - Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China. AD - Guizhou Children's Hospital, Zunyi, Guizhou, China. FAU - Du, Zuochen AU - Du Z AD - Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China. AD - Guizhou Children's Hospital, Zunyi, Guizhou, China. FAU - Huang, Pei AU - Huang P AD - Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China. AD - Guizhou Children's Hospital, Zunyi, Guizhou, China. AD - Guizhou Biomanufacturing Laboratory, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China. FAU - Chen, Yan AU - Chen Y AD - Department of Pediatrics, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China. AD - Guizhou Children's Hospital, Zunyi, Guizhou, China. AD - Guizhou Biomanufacturing Laboratory, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 0 (CD47 Antigen) RN - 0 (Immune Checkpoint Inhibitors) RN - 0 (Receptors, Immunologic) SB - IM MH - Humans MH - *Neoplasms/immunology/therapy/metabolism MH - Animals MH - *Immunotherapy/methods MH - *Phagocytosis/drug effects/immunology MH - CD47 Antigen/immunology/metabolism MH - *Immune Checkpoint Inhibitors/therapeutic use MH - Macrophages/immunology MH - Tumor Escape MH - Translational Research, Biomedical MH - Receptors, Immunologic/metabolism PMC - PMC13711114 OTO - NOTNLM OT - CD47-SIRPalpha axis OT - cancer immunotherapy OT - glyco-immune barrier OT - inhibitory phagocytosis checkpoints OT - macrophages OT - "Don't Eat Me" signals COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:29 PHST- 2026/06/30 00:00 [received] PHST- 2026/08/18 00:00 [revised] PHST- 2026/08/31 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:29 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fimmu.2026.1924227 [doi] PST - epublish SO - Front Immunol. 2026 Oct 13;17:1924227. doi: 10.3389/fimmu.2026.1924227. eCollection 2026. PMID- 42948369 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1792-1015 (Electronic) IS - 1792-0981 (Print) IS - 1792-0981 (Linking) VI - 32 IP - 6 DP - 2026 Dec TI - Hyperbaric oxygen therapy timing, early neurohemodynamic response and functional outcome in acute ischemic stroke. PG - 339 LID - 10.3892/etm.2026.13334 [doi] LID - 339 AB - The optimal timing of HBOT initiation after AIS remains uncertain, particularly in relation to early cerebral hemodynamic responses and subsequent functional outcome. The present study determined whether hyperbaric oxygen therapy (HBOT) initiation timing was associated with early neurohemodynamic response and 90-day functional outcome among patients with acute ischemic stroke (AIS) who received HBOT within 72 h of symptom onset. In the present single-center, retrospective, exposure-stratified cohort study, patients were grouped according to HBOT initiation at <24 h, 24 to <48 h or 48 to 72 h following symptom onset. No non-HBOT comparator was included. The primary outcome was favorable functional status at 90 days, defined as a modified Rankin Scale (mRS) score of 0-2. Secondary analyses assessed early transcranial Doppler (TCD)-defined hemodynamic response, early neurological response, ordinal 90-day mRS shift, an exploratory treatment-emergent integrated early neurohemodynamic response phenotype and safety. Multivariable logistic regression, ordinal logistic regression and robustness analyses used the 48 to 72 h window as the reference. Among the 429 patients, HBOT initiation at <24 h (odds ratio (OR)=2.68; 95% CI, 1.55-4.63; P<0.001) and 24 to <48 h (OR=1.72; 95% CI, 1.04-2.82; P=0.033) was associated with greater odds of a favorable 90-day functional outcome than initiation at 48 to 72 h. Both earlier windows were also associated with greater odds of a lower 90-day mRS category (common OR=2.44; 95% CI, 1.53-3.88; P<0.001; common OR=1.68; 95% CI, 1.09-2.57; P=0.017). TCD hemodynamic response was more frequent in both earlier windows (P<0.001 and P=0.043), whereas a higher rate of early neurological response and a lower rate of early neurological deterioration were observed only in the <24 h window (P=0.003 and P=0.025, respectively). The exploratory integrated early neurohemodynamic response phenotype was associated with favorable functional outcome at 90 days (OR=2.95; 95% CI, 1.76-4.92; P<0.001). Safety outcomes did not differ significantly across initiation windows. Among HBOT-treated patients with AIS, earlier initiation was associated with more favorable early neurohemodynamic and neurological responses and improved 90-day functional outcomes, without evidence of increased safety events. As the present observational study included no non-HBOT comparator, the present findings do not establish HBOT efficacy relative to standard care without HBOT or a causal benefit of earlier initiation. Residual confounding and treatment-selection bias remain possible. CI - Copyright: (c) 2026 Tang et al. FAU - Tang, Xueqing AU - Tang X AD - Department of Neurology, Heyou Hospital, Foshan, Guangdong 528306, P.R. China. FAU - Song, Shaowen AU - Song S AD - Department of Neurology, Heyou Hospital, Foshan, Guangdong 528306, P.R. China. FAU - Wu, Shibo AU - Wu S AD - Department of Neurology, Heyou Hospital, Foshan, Guangdong 528306, P.R. China. FAU - Wang, Yandi AU - Wang Y AD - Department of Neurology, Heyou Hospital, Foshan, Guangdong 528306, P.R. China. FAU - Jin, Guang AU - Jin G AD - Department of Neurology, Heyou Hospital, Foshan, Guangdong 528306, P.R. China. FAU - Ding, Chengyun AU - Ding C AD - Department of Neurology, Heyou Hospital, Foshan, Guangdong 528306, P.R. China. FAU - Zeng, Qinggan AU - Zeng Q AD - Department of Neurology, Heyou Hospital, Foshan, Guangdong 528306, P.R. China. LA - eng PT - Journal Article DEP - 20261013 PL - Greece TA - Exp Ther Med JT - Experimental and therapeutic medicine JID - 101531947 PMC - PMC13711146 OTO - NOTNLM OT - acute ischemic stroke OT - functional outcome OT - hyperbaric oxygen therapy OT - neurohemodynamic response OT - transcranial Doppler OT - treatment timing COIS- The authors declare that they have no competing interests. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:29 PHST- 2026/07/14 00:00 [received] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:29 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - ETM-32-6-13334 [pii] AID - 10.3892/etm.2026.13334 [doi] PST - epublish SO - Exp Ther Med. 2026 Oct 13;32(6):339. doi: 10.3892/etm.2026.13334. eCollection 2026 Dec. PMID- 42948368 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2211-3835 (Print) IS - 2211-3843 (Electronic) IS - 2211-3835 (Linking) VI - 16 IP - 10 DP - 2026 Oct TI - Ultra-sensitive detection of receptor binding domain of SARS-CoV-2 based on artificial intelligence assisted antibody selection and antibody‒nucleic acid conjugation signal amplification. PG - 7075-7085 LID - 10.1016/j.apsb.2026.03.007 [doi] AB - The emergence of SARS-CoV-2 has led to a global pandemic, causing unprecedented disruption to health systems and economies worldwide, calling for an urgent imperative for precise and sensitive diagnostic method. To address this, a novel detection method, termed antibody‒nucleic acid conjugate linked immunosorbent assay (ANCLISA), was designed for the ultra-sensitive detection of the receptor binding domain (RBD) of SARS-CoV-2, which play a pivotal role in the diagnosis of COVID-19. Leveraging the Alphafold 2 model for simulating antibody‒antigen complexes and the DeltaDeltaG predictor model for forecasting the binding free energy of antigen‒antibody complex, we successfully identified the high-affinity antibody TB1. Through the utilization of succinimidyl 4-[N-maleimidomethyl] cyclohexane-1-carboxylate (SMCC) for antibody‒nucleic acid conjugation and the integration of the hybridization chain reaction (HCR) for signal amplification within the system, we have established an ultra-sensitive approach for the SARS-CoV-2 Delta variant detection. The proposed immunoassay provided exhibited a linear detection range spanning 11 fg/mL to 11 ng/mL, achieving a limit of detection (LOD) of 1.48 fg/mL. Our innovative approach represents a significant stride in enabling precise and ultra-sensitive detection of SARS-CoV-2, thereby holding promise for enhancing diagnostic capabilities in the ongoing global battle against the pandemic. CI - (c) 2026 The Authors. FAU - Liu, Jiatong AU - Liu J AD - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. AD - Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, Hangzhou 310052, China. FAU - Chen, Wenfan AU - Chen W AD - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. FAU - Li, Xiufeng AU - Li X AD - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. AD - Innovation Institute for Artificial Intelligence in Medicine, MOE Engineering Research Center of Innovative Anticancer Drugs, Zhejiang Provincial Key Laboratory of Anti-Cancer Drug Research, Zhejiang University, Hangzhou 310018, China. FAU - Luo, Yuanyuan AU - Luo Y AD - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. AD - Innovation Institute for Artificial Intelligence in Medicine, MOE Engineering Research Center of Innovative Anticancer Drugs, Zhejiang Provincial Key Laboratory of Anti-Cancer Drug Research, Zhejiang University, Hangzhou 310018, China. FAU - Li, Qiaomin AU - Li Q AD - Innovation Institute for Artificial Intelligence in Medicine, MOE Engineering Research Center of Innovative Anticancer Drugs, Zhejiang Provincial Key Laboratory of Anti-Cancer Drug Research, Zhejiang University, Hangzhou 310018, China. FAU - Zhao, Wenbin AU - Zhao W AD - Innovation Institute for Artificial Intelligence in Medicine, MOE Engineering Research Center of Innovative Anticancer Drugs, Zhejiang Provincial Key Laboratory of Anti-Cancer Drug Research, Zhejiang University, Hangzhou 310018, China. FAU - He, Qiaojun AU - He Q AD - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. FAU - Zeng, Su AU - Zeng S AD - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. FAU - Zhou, Zhan AU - Zhou Z AD - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. AD - Innovation Institute for Artificial Intelligence in Medicine, MOE Engineering Research Center of Innovative Anticancer Drugs, Zhejiang Provincial Key Laboratory of Anti-Cancer Drug Research, Zhejiang University, Hangzhou 310018, China. AD - The Fourth Affiliated Hospital, Zhejiang University School of Medicine, Yiwu 322000, China. FAU - Cai, Sheng AU - Cai S AD - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. LA - eng PT - Journal Article DEP - 20260310 PL - Netherlands TA - Acta Pharm Sin B JT - Acta pharmaceutica Sinica. B JID - 101600560 PMC - PMC13712282 OTO - NOTNLM OT - AI-assisted antibody selection OT - Antibody‒nucleic acid conjugation OT - Detection OT - Hybridization chain reaction OT - SARS-CoV-2 COIS- All the authors have declared no conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/03/10 CRDT- 2026/10/28 05:29 PHST- 2025/10/21 00:00 [received] PHST- 2025/12/23 00:00 [revised] PHST- 2026/01/06 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:29 [entrez] PHST- 2026/03/10 00:00 [pmc-release] AID - S2211-3835(26)00157-7 [pii] AID - 10.1016/j.apsb.2026.03.007 [doi] PST - ppublish SO - Acta Pharm Sin B. 2026 Oct;16(10):7075-7085. doi: 10.1016/j.apsb.2026.03.007. Epub 2026 Mar 10. PMID- 42948359 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1938-3797 (Electronic) IS - 1086-8089 (Print) IS - 1086-8089 (Linking) VI - 30 IP - 4 DP - 2026 Oct-Dec TI - Long-Term Outcomes of Laparoscopic Total Extraperitoneal Repair for Recurrent Inguinal Hernia. LID - 10.4293/JSLS.2026.00097 [doi] LID - e2026.00097 AB - BACKGROUND: Recurrent inguinal hernia remains a technically demanding clinical problem, particularly following previous anterior mesh repairs. The laparoscopic total extraperitoneal (TEP) approach offers a posterior access route that avoids previously disrupted tissue planes, yet real-world long-term outcome data in this specific population remain limited. METHODS: This retrospective study analyzed 65 patients (74 hernias) who underwent TEP repair for recurrent inguinal hernias between 2012 and 2023. All procedures were performed laparoscopically by a single experienced surgeon at a tertiary referral center using standardized techniques with anatomically preshaped meshes. Patient data were collected from hospital records, and outcomes were evaluated through follow-up periods of up to 141 months. The primary endpoint was rerecurrence rate; secondary endpoints included operative time, length of hospital stay, intraoperative complications, and short-term postoperative complications. RESULTS: The mean operative time was 95.72 minutes, with a mean hospitalization duration of 2.4 days. During a mean follow-up of 57.7 months (range: 8-141 months), no recurrence was reported by patients or documented in the available clinical records. Short-term complications occurred in 8 patients (12.3%), including postoperative pain (n = 4, 6.15%), regional swelling (n = 2, 3.1%), seroma (n = 1, 1.5%), and hematoma (n = 1, 1.5%); all were Clavien-Dindo Grade I-II. No clinically significant chronic postoperative pain was recorded. CONCLUSIONS: The TEP technique demonstrated favorable long-term outcomes in the management of recurrent inguinal hernias, with low complication and recurrence rates. However, its application requires significant surgical expertise, underscoring the importance of surgeon training and experience in achieving optimal results. These findings support TEP as a reliable and effective option for complex recurrent hernia cases in specialized centers. CI - (c) 2026 by SLS, Society of Laparoscopic & Robotic Surgeons. FAU - Sabuncuoglu, M Zafer AU - Sabuncuoglu MZ AD - Department of General Surgery, Faculty of Medicine, Akdeniz University, Antalya, Turkiye. (Drs. Sabuncuoglu and Zihni). FAU - Sozen, Isa AU - Sozen I AD - Department of General Surgery, Faculty of Medicine, Suleyman Demirel University, Isparta, Turkiye. (Drs. Sozen, Turan, Karaca, and Acar). FAU - Turan, Bilal AU - Turan B AD - Department of General Surgery, Faculty of Medicine, Suleyman Demirel University, Isparta, Turkiye. (Drs. Sozen, Turan, Karaca, and Acar). FAU - Karaca, Isa AU - Karaca I AD - Department of General Surgery, Faculty of Medicine, Suleyman Demirel University, Isparta, Turkiye. (Drs. Sozen, Turan, Karaca, and Acar). FAU - Gunduz, Demet AU - Gunduz D AD - Department of Radiology, Faculty of Medicine, Akdeniz University, Antalya, Turkiye. (Dr. Gunduz). FAU - Kirkan Canbaba, Irem AU - Kirkan Canbaba I AD - Department of General Surgery, Malazgirt State Hospital, Mus, Turkiye. (Dr. Canbaba). FAU - Kosan, M Sirac AU - Kosan MS AD - Department of General Surgery, Prof. Dr. A. Ilhan Ozdemir State Hospital, Giresun, Turkiye. (Dr. Kosan). FAU - Zihni, Ismail AU - Zihni I AD - Department of General Surgery, Faculty of Medicine, Akdeniz University, Antalya, Turkiye. (Drs. Sabuncuoglu and Zihni). FAU - Acar, Serdar AU - Acar S AD - Department of General Surgery, Faculty of Medicine, Suleyman Demirel University, Isparta, Turkiye. (Drs. Sozen, Turan, Karaca, and Acar). LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - JSLS JT - JSLS : Journal of the Society of Laparoendoscopic Surgeons JID - 100884618 SB - IM MH - Humans MH - *Hernia, Inguinal/surgery MH - *Laparoscopy/methods MH - Recurrence MH - Retrospective Studies MH - Female MH - *Herniorrhaphy/methods MH - Male MH - Middle Aged MH - Treatment Outcome MH - Aged MH - Postoperative Complications/epidemiology MH - Operative Time MH - Adult MH - Surgical Mesh MH - Length of Stay/statistics & numerical data MH - Follow-Up Studies MH - Aged, 80 and over PMC - PMC13712249 OTO - NOTNLM OT - Laparoscopic TEP repair OT - Minimally invasive hernia surgery OT - Recurrent inguinal hernia OT - Surgical expertise EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/01 CRDT- 2026/10/28 05:29 PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:29 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - JSLS.2026.00097 [pii] AID - 10.4293/JSLS.2026.00097 [doi] PST - ppublish SO - JSLS. 2026 Oct-Dec;30(4):e2026.00097. doi: 10.4293/JSLS.2026.00097. Epub 2026 Oct 27. PMID- 42948356 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1617-9625 (Electronic) IS - 2070-7266 (Print) IS - 1617-9625 (Linking) VI - 24 DP - 2026 TI - Factors associated with smoking cessation among university students, faculty, and staff in Saudi Arabia: A cross-sectional study. LID - 10.18332/tid/231160 [doi] AB - INTRODUCTION: Smoking cessation is an important public health priority given the substantial health risks associated with tobacco use. This study aimed to assess factors associated with former-smoker status among university students, faculty members, and employees in Saudi Arabia. METHODS: A cross-sectional study was conducted at the University of Bisha, Saudi Arabia, from August 2024 to April 2025. Eligible participants were adults affiliated with the university who self-identified as current or former smokers. Data were collected using a self-administered online questionnaire. Associations between participant characteristics and former-smoker status were assessed using Pearson's chi-squared test, followed by univariable and multivariable logistic regression. Because only 48 outcome events were available, the multivariable model was restricted to four conceptually selected variables, with all categorical predictors entered as indicator variables. RESULTS: Among 533 respondents, 113 were current or former smokers; 48 (42.5%) were former smokers and 65 (57.5%) were current smokers. Overall, 83 participants (73.5%) were classified as having quit or attempted to quit smoking. Being classified as having quit or attempted to quit was associated with female sex (p=0.030), non-daily smoking (p=0.009), and cigarettes per day (p=0.023). In univariable analyses, female sex (OR=4.14; 95% CI: 1.54-11.12; p=0.005) and non-daily smoking (OR=6.58; 95% CI: 2.22-19.52; p=0.001) were associated with former-smoker status. In the adjusted analysis, non-daily smoking remained the only independent association (AOR=5.47; 95% CI: 1.60-18.68; p=0.007). The association with female sex was attenuated and no longer statistically significant (AOR=3.01; 95% CI: 0.97-9.36; p=0.057), whereas neither age nor any cigarettes per day category remained independently associated with former-smoker status. CONCLUSIONS: Non-daily smoking was the only factor independently associated with former-smoker status. These findings are exploratory and limited by the single-university convenience sample and the imprecision of some estimates. Larger longitudinal studies using validated measures of tobacco exposure and sustained abstinence are needed to confirm these associations. CI - (c) 2026 Alshahrani A.F. et al. FAU - Alshahrani, Abdulaziz F AU - Alshahrani AF AD - Department of Family and Community Medicine, College of Medicine, University of Bisha, Bisha, Saudi Arabia. FAU - Alhalafi, Abdullah H AU - Alhalafi AH AD - Department of Family and Community Medicine, College of Medicine, University of Bisha, Bisha, Saudi Arabia. FAU - Alshahrani, Mubarak F AU - Alshahrani MF AD - Department of Emergency Medicine, Security Forces Hospital, Riyadh, Saudi Arabia. FAU - Alshahrani, Saeed F AU - Alshahrani SF AD - Medicine and Surgery Department, King Khalid University, Saudi Board of Family Medicine, Abha, Saudi Arabia. FAU - Abuliat, Aws A AU - Abuliat AA AD - Department of Family and Community Medicine, College of Medicine, University of Bisha, Bisha, Saudi Arabia. FAU - Alqahtani, Mubarak A AU - Alqahtani MA AD - Department of Family and Community Medicine, College of Medicine, University of Bisha, Bisha, Saudi Arabia. FAU - Alharthi, Mohammed A AU - Alharthi MA AD - Department of Family and Community Medicine, College of Medicine, University of Bisha, Bisha, Saudi Arabia. FAU - Alsoiry, Nawaf A AU - Alsoiry NA AD - Department of Family and Community Medicine, College of Medicine, University of Bisha, Bisha, Saudi Arabia. FAU - Alsaadi, Mashael O AU - Alsaadi MO AD - Department of Family and Community Medicine, College of Medicine, University of Bisha, Bisha, Saudi Arabia. FAU - Alshahrani, Mohammed M AU - Alshahrani MM AD - Department of Family and Community Medicine, College of Medicine, University of Bisha, Bisha, Saudi Arabia. FAU - Alshomrani, Raydaa A AU - Alshomrani RA AD - Department of Family and Community Medicine, College of Medicine, University of Bisha, Bisha, Saudi Arabia. FAU - Alghamdi, Huthayfah A AU - Alghamdi HA AD - Department of Family and Community Medicine, College of Medicine, University of Bisha, Bisha, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261026 PL - Greece TA - Tob Induc Dis JT - Tobacco induced diseases JID - 101201591 PMC - PMC13712268 OTO - NOTNLM OT - Saudi Arabia OT - associated factors OT - smoking cessation OT - tobacco use OT - university population COIS- The authors have completed and submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest and none was reported. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/26 CRDT- 2026/10/28 05:28 PHST- 2026/07/22 00:00 [received] PHST- 2026/08/12 00:00 [revised] PHST- 2026/08/19 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:28 [entrez] PHST- 2026/10/26 00:00 [pmc-release] AID - TID-24-198 [pii] AID - 10.18332/tid/231160 [doi] PST - epublish SO - Tob Induc Dis. 2026 Oct 26;24. doi: 10.18332/tid/231160. eCollection 2026. PMID- 42948354 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2211-3835 (Print) IS - 2211-3843 (Electronic) IS - 2211-3835 (Linking) VI - 16 IP - 10 DP - 2026 Oct TI - Antibody‒cell conjugates: A novel "off-the-shelf" modality bridging ADCs and CAR-T for cancer immunotherapy. PG - 6360-6377 LID - 10.1016/j.apsb.2026.07.034 [doi] AB - Antibody‒cell conjugates (ACCs) represent a promising therapeutic strategy emerging in the biopharmaceutical industry to overcome the limitations of current immunotherapies. ACCs constitute a novel class of living drugs developed by anchoring functional antibodies onto the surface of living cells via specific linkers or conjugation methods. These carrier cells encompass both immune effector cells (such as Natural Killer cells, gammadelta T cells, and activated T cells) and non-immune cells (such as erythrocytes and platelets). Distinct from genetic engineering, ACCs directly modify the cell surface using chemical biology, enzymatic catalysis, or physical assembly, thereby endowing cells with precise tumor-targeting capabilities while preserving their intrinsic biological functions. This technology facilitates the scalable production of "off-the-shelf" therapeutics and has shown promising potential in treating both hematological malignancies and refractory solid tumors with a superior safety profile. This review systematically examines the current construction strategies and carrier cell selection for ACCs, covering both immune effector cells and non-immune carrier cells, and discusses the clinical progress, key challenges, and future directions. CI - (c) 2026 The Authors. FAU - Dai, Yifei AU - Dai Y AD - Yangtze River Delta Center for Drug Evaluation and Inspection of The National Medical Products Administration, Shanghai 201210, China. FAU - Xie, Yujia AU - Xie Y AD - Yangtze River Delta Center for Drug Evaluation and Inspection of The National Medical Products Administration, Shanghai 201210, China. FAU - Ma, Xiaojuan AU - Ma X AD - Yangtze River Delta Center for Drug Evaluation and Inspection of The National Medical Products Administration, Shanghai 201210, China. FAU - Zhang, Dong AU - Zhang D AD - Yangtze River Delta Center for Drug Evaluation and Inspection of The National Medical Products Administration, Shanghai 201210, China. FAU - Yang, Jiao AU - Yang J AD - Yangtze River Delta Center for Drug Evaluation and Inspection of The National Medical Products Administration, Shanghai 201210, China. FAU - Lin, Kequan AU - Lin K AD - Department of Cardiology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China. FAU - Chen, Aiping AU - Chen A AD - Yangtze River Delta Center for Drug Evaluation and Inspection of The National Medical Products Administration, Shanghai 201210, China. FAU - Qiang, Weijie AU - Qiang W AD - Department of Pulmonary and Critical Care Medicine, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China. LA - eng PT - Journal Article PT - Review DEP - 20260725 PL - Netherlands TA - Acta Pharm Sin B JT - Acta pharmaceutica Sinica. B JID - 101600560 PMC - PMC13712271 OTO - NOTNLM OT - Allogeneic cell therapy OT - Antibody‒cell conjugates OT - Cancer immunotherapy OT - Carrier cells OT - Clinical progress OT - Construction strategies OT - Non-genetic engineering OT - Off-the-shelf COIS- The authors declare no competing interests. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/07/25 CRDT- 2026/10/28 05:28 PHST- 2026/02/01 00:00 [received] PHST- 2026/04/18 00:00 [revised] PHST- 2026/07/19 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:28 [entrez] PHST- 2026/07/25 00:00 [pmc-release] AID - S2211-3835(26)00475-2 [pii] AID - 10.1016/j.apsb.2026.07.034 [doi] PST - ppublish SO - Acta Pharm Sin B. 2026 Oct;16(10):6360-6377. doi: 10.1016/j.apsb.2026.07.034. Epub 2026 Jul 25. PMID- 42948353 OWN - NLM STAT- Publisher LR - 20261029 IS - 2041-6520 (Print) IS - 2041-6539 (Electronic) IS - 2041-6520 (Linking) DP - 2026 Oct 21 TI - Interplay of singlet and triplet excited states in photoredox catalysis. LID - 10.1039/d6sc04715g [doi] AB - Photocatalysis has emerged as a rapidly expanding field, offering promising strategies for driving challenging and thermodynamically uphill transformations through the use of light. By harnessing the unique reactivity of electronically excited states, photocatalysis requires the coupling of the rigorous mechanistic approach of photophysics with the synthetic insight of organic chemistry. This perspective provides a conceptual framework for understanding fundamental aspects of photoredox catalysis, with a focus on the competitive roles of singlet (S(1)) and triplet (T(1)) excited states of the photocatalyst in determining overall catalytic efficiency. We analyse how key parameters, including photophysical properties of the photocatalyst (e.g., intersystem crossing efficiency and lifetimes of excited states) and reactant concentration modulate the balance between singlet and triplet-mediated pathways under catalytic conditions. Particular attention is given to how dynamic changes in reaction mixtures and quenching processes can shift the relative contribution of these excited states over time, thereby influencing overall performance. Selected literature examples are used to illustrate how these factors translate into observable differences in reactivity, selectivity, and efficiency across representative photoredox transformations. We thus aim to provide mechanistic insights and kinetic modelling that bridge photophysical understanding and synthetic application, ultimately guiding the rational design of more efficient photoredox systems and optimized reaction conditions. CI - This journal is (c) The Royal Society of Chemistry. FAU - Bellatreccia, Caterina AU - Bellatreccia C AD - Dipartimento di Chimica "Giacomo Ciamician", Alma Mater Studiorum - Universita di Bologna via Pietro Gobetti 85 40129 Bologna Italy paola.ceroni@unibo.it. FAU - Fina, Federica AU - Fina F AUID- ORCID: 0009-0000-6199-051X AD - Dipartimento di Chimica "Giacomo Ciamician", Alma Mater Studiorum - Universita di Bologna via Pietro Gobetti 85 40129 Bologna Italy paola.ceroni@unibo.it. FAU - Ceroni, Paola AU - Ceroni P AUID- ORCID: 0000-0001-8916-1473 AD - Dipartimento di Chimica "Giacomo Ciamician", Alma Mater Studiorum - Universita di Bologna via Pietro Gobetti 85 40129 Bologna Italy paola.ceroni@unibo.it. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - England TA - Chem Sci JT - Chemical science JID - 101545951 PMC - PMC13711120 COIS- There are no conflicts to declare. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/21 CRDT- 2026/10/28 05:28 PHST- 2026/06/05 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:28 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - d6sc04715g [pii] AID - 10.1039/d6sc04715g [doi] PST - aheadofprint SO - Chem Sci. 2026 Oct 21. doi: 10.1039/d6sc04715g. PMID- 42948349 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1663-9812 (Print) IS - 1663-9812 (Electronic) IS - 1663-9812 (Linking) VI - 17 DP - 2026 TI - Mechanisms of traditional Chinese medicine interventions in the gastric 'inflammation-to-carcinoma' cascade: advances in in vitro models from monolayer cells to organoids. PG - 1906847 LID - 10.3389/fphar.2026.1906847 [doi] LID - 1906847 AB - Gastric cancer remains a leading cause of cancer-related mortality worldwide. The Correa cascade, progressing from chronic gastritis through precancerous lesions to adenocarcinoma, provides the framework for understanding gastric mucosal stepwise malignant transformation. Traditional Chinese Medicine (TCM) formulas, such as Zuojin Pills and Wuji Pills, have been historically used to treat gastric disorders including epigastric pain and acid regurgitation, yet their mechanisms of action in blocking the inflammation-to-carcinoma transition remain incompletely understood. The mechanistic diversity of TCM presents challenges for in vitro modeling, and existing reviews have not systematically addressed how model selection should align with specific research questions across different stages of the cascade. This review examines the evolving landscape of in vitro models used in TCM research on gastric mucosal inflammation-to-carcinoma transition, highlighting the paradigm shift from two-dimensional (2D) monolayer GES-1 cell cultures to three-dimensional gastric organoids. We analyzed recent studies conducted across multiple model platforms using various acute or chronic pathogenic factors, with all botanical drugs validated taxonomically through authoritative sources. The GES-1 cell model offers low cost, high throughput, and genetic homogeneity, making it suitable for initial screening of active metabolites from botanical drugs and for dissecting molecular pathways, particularly those involving Nrf2, NF-kappaB, PI3K/AKT, and MAPK signaling in antioxidative stress, anti-inflammatory, and anti-apoptotic mechanisms. In contrast, gastric organoids preserve three-dimensional architecture, cellular diversity, and patient-specific genetic backgrounds, enabling functional assessment of barrier integrity, stem cell-driven regeneration, and malignant transformation at the tissue level. To address the need for evidence differentiation, we introduce a hierarchical framework (Model Evidence Level) in which GES-1 cells serve for entry-level target discovery and patient-derived organoids for higher-level tissue validation. Based on this framework, we provide stage-specific model selection recommendations corresponding to the distinct phases of the Correa cascade: acute inflammation, chronic atrophy, intestinal metaplasia, dysplasia, and gastric cancer. This review offers a practical, evidence-stratified pathway for in vitro model selection in TCM intervention studies, bridging traditional ethnopharmacological knowledge with modern pharmacological validation to strengthen the translational relevance of preclinical findings. CI - Copyright (c) 2026 Zhang, Wang, Zeng, Zhou and Zhao. FAU - Zhang, Xiaoling AU - Zhang X AD - Department of Spleen and Stomach Diseases, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, Sichuan, China. FAU - Wang, Tiangang AU - Wang T AD - Department of Spleen and Stomach Diseases, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, Sichuan, China. FAU - Zeng, Xusen AU - Zeng X AD - Department of Spleen and Stomach Diseases, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, Sichuan, China. FAU - Zhou, Xin AU - Zhou X AD - Department of Spleen and Stomach Diseases, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, Sichuan, China. FAU - Zhao, Long AU - Zhao L AD - Department of Spleen and Stomach Diseases, The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University, Luzhou, Sichuan, China. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Pharmacol JT - Frontiers in pharmacology JID - 101548923 PMC - PMC13711113 OTO - NOTNLM OT - Correa cascade OT - GES-1 cells OT - gastric organoids OT - in vitro models OT - inflammation to carcinoma transition OT - traditional Chinese medicine COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:28 PHST- 2026/06/11 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:28 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 1906847 [pii] AID - 10.3389/fphar.2026.1906847 [doi] PST - epublish SO - Front Pharmacol. 2026 Oct 13;17:1906847. doi: 10.3389/fphar.2026.1906847. eCollection 2026. PMID- 42948347 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2211-3835 (Print) IS - 2211-3843 (Electronic) IS - 2211-3835 (Linking) VI - 16 IP - 10 DP - 2026 Oct TI - Intelligent nano delivery systems: Classification, stimuli-responsive mechanisms, and therapeutic applications in critical illnesses. PG - 6309-6337 LID - 10.1016/j.apsb.2026.06.015 [doi] AB - Critical illnesses such as sepsis and acute respiratory distress syndrome (ARDS) evolve rapidly and are dominated by systemic inflammatory dysregulation, oxidative stress, and abrupt microenvironmental remodeling, which collectively narrow the therapeutic window and amplify treatment-related toxicity. Conventional pharmacotherapy is often limited by non-selective biodistribution, insufficient lesion exposure, and dose-limiting adverse effects. Intelligent nano-delivery systems (INDS) address these barriers by coupling pathological cues (e.g., pH gradients, redox/ROS imbalance, and disease-associated enzymes) to defined carrier-state transitions, thereby enabling lesion-confined activation, controlled bioavailability, and, in selected designs, real-time readouts. In this review, we summarize the design logic of stimulus-responsive and targeting modules, and critically discuss how they translate endogenous signals into payload release or functional actuation in representative critical-illness settings. We further highlight emerging directions, including self-adaptive multi-input gating and biomimetic interfaces, and discuss key translational constraints (manufacturability, safety, and reproducibility) that must be addressed to advance INDS toward critical-care practice. CI - (c) 2026 The Authors. FAU - Zhao, Mengxin AU - Zhao M AD - College of Basic Medical Sciences, The Medical Basic Research Innovation Center of Airway Disease in North China, Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun 130021, China. AD - Department of Surgery, China-Japan Union Hospital, Jilin University, Changchun 130021, China. FAU - Guo, Baofeng AU - Guo B AD - Department of Surgery, China-Japan Union Hospital, Jilin University, Changchun 130021, China. FAU - Wang, Quanhao AU - Wang Q AD - College of Basic Medical Sciences, The Medical Basic Research Innovation Center of Airway Disease in North China, Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun 130021, China. FAU - Yang, Mei AU - Yang M AD - College of Basic Medical Sciences, The Medical Basic Research Innovation Center of Airway Disease in North China, Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun 130021, China. FAU - Yang, Jiaying AU - Yang J AD - College of Basic Medical Sciences, The Medical Basic Research Innovation Center of Airway Disease in North China, Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun 130021, China. FAU - Liang, Yueyang AU - Liang Y AD - College of Basic Medical Sciences, The Medical Basic Research Innovation Center of Airway Disease in North China, Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun 130021, China. FAU - Jiang, Wei AU - Jiang W AD - State Key Laboratory of Metabolic Dysregulation & Prevention and Treatment of Esophageal Cancer, Tianjian Laboratory of Advanced Biomedical Sciences, School of Convergence Medicine, Zhengzhou University, Zhengzhou 450052, China. FAU - Zhang, Ling AU - Zhang L AD - College of Basic Medical Sciences, The Medical Basic Research Innovation Center of Airway Disease in North China, Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun 130021, China. LA - eng PT - Journal Article PT - Review DEP - 20260618 PL - Netherlands TA - Acta Pharm Sin B JT - Acta pharmaceutica Sinica. B JID - 101600560 PMC - PMC13712275 OTO - NOTNLM OT - Conventional pharmacotherapy OT - Critical illnesses OT - Design logic OT - Emerging directions OT - Intelligent nano delivery systems (INDS) OT - Pathological cues OT - Stimulus-responsive and targeting modules OT - Translational constraints COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/06/18 CRDT- 2026/10/28 05:28 PHST- 2025/09/30 00:00 [received] PHST- 2026/02/04 00:00 [revised] PHST- 2026/05/06 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:28 [entrez] PHST- 2026/06/18 00:00 [pmc-release] AID - S2211-3835(26)00344-8 [pii] AID - 10.1016/j.apsb.2026.06.015 [doi] PST - ppublish SO - Acta Pharm Sin B. 2026 Oct;16(10):6309-6337. doi: 10.1016/j.apsb.2026.06.015. Epub 2026 Jun 18. PMID- 42948342 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 17 DP - 2026 TI - Natural killer cell-derived extracellular vesicles in cancer: mediators of intercellular communication and tumor immune signaling. PG - 1919867 LID - 10.3389/fimmu.2026.1919867 [doi] LID - 1919867 AB - Natural killer (NK) cells are essential components of innate immunity and play a pivotal role in tumor surveillance through direct cytotoxicity and immune regulation. In recent years, NK-cell-derived extracellular vesicles (NK-EVs) have emerged as a promising cell-free therapeutic modality that preserves key antitumor functions of parental NK cells while overcoming several limitations associated with cell-based immunotherapy. As biologically derived nanovesicles, NK-EVs combine nanoscale delivery properties with a cell-free formulation format and generally low immunogenicity. However, although selected preclinical studies suggest tumor-interacting or target-cell-recognition properties, a broadly applicable intrinsic tumor-targeting capacity-and, in particular, intrinsic blood-brain barrier (BBB) penetration-has not been established for native NK-EVs. This review systematically summarizes the biological characteristics, biogenesis pathways, and molecular cargo of NK-EVs, with particular emphasis on their antitumor mechanisms. Current evidence supports perforin/granzyme-mediated cytotoxicity and Fas/Fas ligand (FasL) signaling as the most established mechanisms of NK-EVs-mediated tumor killing, whereas miRNA-mediated regulation and tumor microenvironment remodeling represent emerging functional pathways requiring further validation. By contrast, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-death receptor 4/5 (DR4/DR5) signaling remains putative because direct NK-EVs-specific causal evidence is limited. Functionally investigated miRNAs and tumor-microenvironment effects represent context-dependent or emerging pathways whose generalizability across NK-cell sources and preparation conditions requires further validation. Importantly, recent advances in engineering strategies-including parental-cell priming, cargo loading, antigen-recognition engineering, tissue/barrier-targeting modification, biomimetic nanovesicle construction, and formulation-based stabilization. These approaches can alter cargo composition, recipient-cell recognition, or tissue-specific delivery through distinct mechanisms. Improvements achieved by engineered composite platforms should therefore not be conflated with intrinsic properties of native NK-EVs. Nevertheless, major translational challenges remain, including scalable manufacturing, purification and characterization standardization, mechanism-linked potency testing, biodistribution uncertainty, batch comparability, and product-specific regulatory considerations. Overall, NK-EVs should be viewed not only as potential cell-free therapeutic tools but also as specialized mediators of tumor-immune communication that integrate cytotoxic protein transfer, receptor-mediated signaling, RNA-based regulation, and microenvironmental crosstalk. CI - Copyright (c) 2026 Liu, Yang, Zhang, Liu, Yu, Song, Liao, Jiang, Guo and Huang. FAU - Liu, Jun AU - Liu J AD - Department of Neurosurgery, The 2nd Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, China. FAU - Yang, Yancong AU - Yang Y AD - Department of Neurosurgery, The Affiliated Ganzhou Hospital, Jiangxi Medical College, Nanchang University, Ganzhou, Jiangxi, China. FAU - Zhang, Wenjun AU - Zhang W AD - Department of Rehabilitation Medicine, The Affiliated Ganzhou Hospital, Jiangxi Medical College, Nanchang University, Ganzhou, Jiangxi, China. FAU - Liu, Jianwu AU - Liu J AD - Department of Neurosurgery, The Affiliated Ganzhou Hospital, Jiangxi Medical College, Nanchang University, Ganzhou, Jiangxi, China. FAU - Yu, Yanyan AU - Yu Y AD - Department of Neurosurgery, The 2nd Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, China. FAU - Song, Ye AU - Song Y AD - Department of Neurosurgery, Guangzhou Women and Children's Medical Center, Guangzhou, Guangdong, China. FAU - Liao, Xiaofei AU - Liao X AD - Department of Pharmacy, Ganzhou People's Hospital, Ganzhou, Jiangxi, China. FAU - Jiang, Qiuhua AU - Jiang Q AD - Department of Neurosurgery, The Affiliated Ganzhou Hospital, Jiangxi Medical College, Nanchang University, Ganzhou, Jiangxi, China. FAU - Guo, Hua AU - Guo H AD - Department of Neurosurgery, The 2nd Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, China. FAU - Huang, Qianliang AU - Huang Q AD - Department of Neurosurgery, The Affiliated Ganzhou Hospital, Jiangxi Medical College, Nanchang University, Ganzhou, Jiangxi, China. LA - eng PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 0 (MicroRNAs) SB - IM MH - Humans MH - *Killer Cells, Natural/immunology/metabolism MH - *Extracellular Vesicles/immunology/metabolism MH - *Neoplasms/immunology/therapy/pathology/metabolism MH - *Cell Communication/immunology MH - Signal Transduction/immunology MH - Animals MH - Tumor Microenvironment/immunology MH - MicroRNAs PMC - PMC13712155 OTO - NOTNLM OT - intercellular communication OT - miRNA-mediated regulation OT - natural killer cell-derived extracellular vesicles (NK-EVs) OT - tumor immune signaling OT - tumor microenvironment COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/12 CRDT- 2026/10/28 05:27 PHST- 2026/06/25 00:00 [received] PHST- 2026/08/16 00:00 [revised] PHST- 2026/08/24 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:27 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - 10.3389/fimmu.2026.1919867 [doi] PST - epublish SO - Front Immunol. 2026 Oct 12;17:1919867. doi: 10.3389/fimmu.2026.1919867. eCollection 2026. PMID- 42948341 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-462X (Print) IS - 1664-462X (Electronic) IS - 1664-462X (Linking) VI - 17 DP - 2026 TI - Deep learning-based estimation of Kent mango yield by commercial export category under real field conditions in northern Peru. PG - 1943711 LID - 10.3389/fpls.2026.1943711 [doi] LID - 1943711 AB - Manual visual counting remains the standard procedure for estimating Kent mango (Mangifera indica L.) yield in northern Peru, an inconsistent practice that compromises harvest planning, transport dimensioning and commercial negotiation. Existing computer vision approaches to mango detection resolve fruit-versus-background discrimination but rarely classify fruit into the commercial categories that determine its market value. This study developed and validated a deep learning method that simultaneously detects and classifies Kent mango fruit into three export categories (air freight, sea freight and reject) from RGB images acquired under real field conditions in the Motupe district, Lambayeque. A purpose-built dataset of 400 photographs was compiled from 200 trees across ten commercial farms using two opposite views per tree; 6,544 patches of 1,280 x 1,280 pixels with 20% overlap were generated, containing 66,465 manually annotated instances. Five architectures were trained under identical experimental conditions: YOLOv8m, YOLO11m, YOLO26m, RT-DETR-l and Mango-YOLO, the latter a proposed architecture built on YOLO26m through the incorporation of a CBAM attention module, a BiFPN neck and the MPDIoU regression loss. Mango-YOLO achieved the best performance across all five metrics, with a precision of 0.930, a recall of 0.940, an F1-score of 0.930, a mAP@0.5 of 0.940 and a mAP@0.5:0.95 of 0.730, at an inference time of 114.94 ms per image. Per-category analysis revealed heterogeneous behaviour, with F1-scores of 0.960, 0.845 and 0.792 for sea freight, air freight and reject respectively. The selected model was integrated into a three-tier web application that scales fruit counts from sampled trees to the whole farm and converts them into kilograms through Monte Carlo simulation with category mean weights, returning a 90% interval. Validation against harvested production recorded on the processing-plant scale across the ten farms yielded a mean absolute percentage error of 2.63% and a coefficient of determination of 0.9993, outperforming the field supervisor's manual estimate by a factor of 3.3. CI - Copyright (c) 2026 Huaman Asencio, Mejia Cabrera and Arcila Diaz. FAU - Huaman Asencio, Victor Yoel AU - Huaman Asencio VY AD - Escuela Profesional de Ingenieria de Sistemas, Facultad de Ingenieria, Arquitectura y Urbanismo, Universidad Senor de Sipan, Lambayeque, Peru. FAU - Mejia Cabrera, Heber Ivan AU - Mejia Cabrera HI AD - Escuela Profesional de Ingenieria de Sistemas, Facultad de Ingenieria, Arquitectura y Urbanismo, Universidad Senor de Sipan, Lambayeque, Peru. FAU - Arcila Diaz, Juan Carlos AU - Arcila Diaz JC AD - Escuela Profesional de Ingenieria de Sistemas, Facultad de Ingenieria, Arquitectura y Urbanismo, Universidad Senor de Sipan, Lambayeque, Peru. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Plant Sci JT - Frontiers in plant science JID - 101568200 PMC - PMC13712225 OTO - NOTNLM OT - Kent mango OT - YOLO OT - computer vision OT - convolutional attention OT - export category classification OT - object detection OT - precision agriculture OT - yield estimation COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:27 PHST- 2026/07/21 00:00 [received] PHST- 2026/08/28 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:27 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fpls.2026.1943711 [doi] PST - epublish SO - Front Plant Sci. 2026 Oct 13;17:1943711. doi: 10.3389/fpls.2026.1943711. eCollection 2026. PMID- 42948340 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2042-8812 (Print) IS - 2042-8812 (Electronic) IS - 2042-8812 (Linking) VI - 2026 IP - 10 DP - 2026 Oct TI - Orthotopic heart transplantation associated with hemiarch reconstruction for advanced heart failure and chronic aortic dissection: a case report. PG - rjag775 LID - 10.1093/jscr/rjag775 [doi] LID - rjag775 AB - Concomitant orthotopic heart transplantation and aortic arch replacement are extremely rare, particularly in chronic Stanford type A dissection. A 43-year-old man with end-stage heart failure and chronic aortic dissection underwent combined orthotopic heart transplantation and hemiarch replacement. Because of concomitant liver donation, the donor inferior vena cava required pericardial patch reconstruction. Despite early right ventricular dysfunction requiring veno-arterial ECMO, the patient recovered completely and was discharged with preserved graft function. This case demonstrates the feasibility of a single-stage combined approach with excellent postoperative outcomes in carefully selected patients. CI - (c) The Author(s) 2026. Published by Oxford University Press and JSCR Publishing Ltd. FAU - Sanchez, Oscar Edinson AU - Sanchez OE AD - Cardiovascular Surgery Section, Fundacion Clinica Shaio, 111131, Bogota, Colombia. FAU - Perez-Ariza, Valentina AU - Perez-Ariza V AUID- ORCID: 0000-0002-2031-730X AD - School of Medicine, Universidad de los Andes, 111711, Bogota, Colombia. AD - School of Medicine, Department of Surgery, 110231, Pontificia Universidad Javeriana, Bogota, Colombia. FAU - Cruz Buitrago, Roberto H AU - Cruz Buitrago RH AD - Cardiovascular Surgery Section, Fundacion Clinica Shaio, 111131, Bogota, Colombia. AD - School of Medicine, Department of Surgery, 110231, Pontificia Universidad Javeriana, Bogota, Colombia. FAU - Parra, Juan F AU - Parra JF AD - Cardiovascular Surgery Section, Fundacion Clinica Shaio, 111131, Bogota, Colombia. FAU - Rios, William AU - Rios W AD - Cardiovascular Surgery Section, Fundacion Clinica Shaio, 111131, Bogota, Colombia. FAU - Nunez, Federico AU - Nunez F AD - Cardiovascular Surgery Section, Fundacion Clinica Shaio, 111131, Bogota, Colombia. LA - eng PT - Case Reports PT - Journal Article DEP - 20261027 PL - England TA - J Surg Case Rep JT - Journal of surgical case reports JID - 101560169 PMC - PMC13711083 OTO - NOTNLM OT - aortic arch reconstruction OT - aortic dissection OT - heart transplant COIS- None declared. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/27 CRDT- 2026/10/28 05:27 PHST- 2026/07/10 00:00 [received] PHST- 2026/08/13 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:27 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - rjag775 [pii] AID - 10.1093/jscr/rjag775 [doi] PST - epublish SO - J Surg Case Rep. 2026 Oct 27;2026(10):rjag775. doi: 10.1093/jscr/rjag775. eCollection 2026 Oct. PMID- 42948326 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1177-8881 (Electronic) IS - 1177-8881 (Linking) VI - 20 DP - 2026 TI - MiR-532 as a Potential Therapeutic Target and Biomarker: Biological Functions, Molecular Mechanisms, and Clinical Perspectives. PG - 637896 LID - 10.2147/DDDT.S637896 [doi] LID - 637896 AB - MicroRNA-532 (miR-532), including miR-532-3p and miR-532-5p, is an important regulator of gene expression involved in numerous physiological and pathological processes. It modulates key signaling pathways, including PI3K/Akt and Wnt/beta-catenin, thereby contributing to the regulation of several biological processes, including oxidative stress, inflammation, apoptosis, cell proliferation, migration, and invasion. Dysregulation of miR-532 is associated with various diseases, including neurological disorders, cardiovascular diseases, and musculoskeletal disorders, as well as cancer. miR-532 modulates inflammation, oxidative stress, and cell death. Its role in cancer appears to be context-dependent, functioning as either a tumor suppressor or an oncogenic regulator depending on the tumor type. In addition, miR-532 has been detected in tissues and body fluids, and its altered expression has been reported in several diseases, suggesting its potential as a candidate biomarker for disease-related changes. This narrative review summarizes relevant literature identified through searches of PubMed, Scopus, and Web of Science for studies published up to June 2026. The focus of this review is on the biological functions, molecular mechanisms, and therapeutic and diagnostic significance of miR-532 in human diseases. However, current findings are largely based on preclinical and observational evidence, with relatively few clinical studies available. Overall, miR-532 shows potential as a therapeutic target and biomarker, but further clinical studies are needed to confirm its clinical significance. CI - (c) 2026 Mohammadi et al. FAU - Mohammadi, Leila AU - Mohammadi L AUID- ORCID: 0009-0009-9334-493X AD - Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. FAU - Baluchnejadmojarad, Tourandokht AU - Baluchnejadmojarad T AD - Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. FAU - Roghani, Mehrdad AU - Roghani M AUID- ORCID: 0000-0002-9209-8484 AD - Neurophysiology Research Center, Shahed University, Tehran, Iran. LA - eng PT - Journal Article PT - Review DEP - 20261023 PL - New Zealand TA - Drug Des Devel Ther JT - Drug design, development and therapy JID - 101475745 RN - 0 (MicroRNAs) RN - 0 (MIRN532 microRNA, human) RN - 0 (Biomarkers) SB - IM MH - Humans MH - *MicroRNAs/metabolism/genetics/antagonists & inhibitors MH - Biomarkers/metabolism/analysis MH - Animals MH - *Neoplasms/metabolism/drug therapy/diagnosis PMC - PMC13712144 OTO - NOTNLM OT - apoptosis OT - diagnosis OT - inflammation OT - miR-532 OT - oxidative stress OT - prognosis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/23 CRDT- 2026/10/28 05:26 PHST- 2026/07/04 00:00 [received] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:26 [entrez] PHST- 2026/10/23 00:00 [pmc-release] AID - 637896 [pii] AID - 10.2147/DDDT.S637896 [doi] PST - epublish SO - Drug Des Devel Ther. 2026 Oct 23;20:637896. doi: 10.2147/DDDT.S637896. eCollection 2026. PMID- 42948319 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2090-6986 (Print) IS - 2090-6994 (Electronic) IS - 2090-6994 (Linking) VI - 2026 DP - 2026 TI - Left Internal Carotid Artery Dissection Secondary to Minor Trauma: A Case Report Involving "Headbanging" in a Heavy Metal Band. PG - 9589977 LID - 10.1155/crvm/9589977 [doi] LID - 9589977 AB - INTRODUCTION: Internal carotid artery dissection (ICAD) is an uncommon but clinically significant cause of ischemic stroke in young adults, accounting for approximately 10%-25% of cases in this population. It is frequently associated with minor trauma or repetitive cervical movements. Headbanging, characteristic of heavy metal music performances, involves vigorous, repetitive movements of the head and neck and may increase the risk of cervical vascular injury, including ICAD. CASE REPORT: A 37-year-old male musician with no prior neurological disease presented with severe headache and transient focal neurological symptoms following heavy metal performances involving headbanging. On examination, blood pressure was 130/80 mmHg, and no focal neurological deficits were observed (NIHSS score: 0). Computed tomography angiography demonstrated a subocclusive thrombus involving the distal cervical and petrous segments of the left internal carotid artery, with distal hypoperfusion. Digital subtraction angiography subsequently confirmed a left ICAD of the petrous segment with approximately 80% luminal stenosis. Management included anticoagulation with dabigatran, strict blood pressure control, and multidisciplinary follow-up, including psychological support. The patient was discharged with guidance to avoid abrupt cervical movements and to continue regular outpatient and imaging follow-up. DISCUSSION: Although uncommon, the association between headbanging and ICAD warrants consideration in young patients presenting with ischemic stroke in the absence of traditional cardiovascular risk factors. Repetitive, forceful cervical movements may cause arterial wall injury, predisposing to dissection. Early diagnosis is essential to guide appropriate management and prevent complications. CONCLUSION: This case highlights the importance of recognizing activities involving repetitive, vigorous cervical movements, such as headbanging, as potential etiological factors for arterial dissection in young adults. The absence of follow-up imaging is a limitation of this case report; nonetheless, it remains useful in raising awareness that may facilitate earlier diagnosis and improve clinical outcomes. CI - Copyright (c) 2026 Louissa Srama Rosner Cidral et al. Case Reports in Vascular Medicine published by John Wiley & Sons Ltd. FAU - Cidral, Louissa Srama Rosner AU - Cidral LSR AUID- ORCID: 0009-0006-3132-8641 AD - Neurology and Neurosurgery Department NeuroDOC Curitiba Brazil. FAU - Nakoula, Eduarda AU - Nakoula E AUID- ORCID: 0009-0008-6253-352X AD - Neurology and Neurosurgery Department NeuroDOC Curitiba Brazil. FAU - Merida, Kristel Back AU - Merida KB AUID- ORCID: 0000-0002-8144-9365 AD - Neurology and Neurosurgery Department NeuroDOC Curitiba Brazil. AD - Neurology and Neurosurgery Department Hospital Santa Cruz Curitiba Parana Brazil. FAU - Andrade, Ana Carolina AU - Andrade AC AD - Neurology and Neurosurgery Department NeuroDOC Curitiba Brazil. AD - Neurology and Neurosurgery Department Hospital Santa Cruz Curitiba Parana Brazil. FAU - Penzo, Luiz AU - Penzo L AUID- ORCID: 0009-0006-6463-5058 AD - Neurology and Neurosurgery Department NeuroDOC Curitiba Brazil. AD - Neurology and Neurosurgery Department Hospital Santa Cruz Curitiba Parana Brazil. FAU - Ruschel, Leonardo Gilmone AU - Ruschel LG AUID- ORCID: 0000-0002-5005-0271 AD - Neurology and Neurosurgery Department NeuroDOC Curitiba Brazil. AD - Neurology and Neurosurgery Department Hospital Santa Cruz Curitiba Parana Brazil. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Case Rep Vasc Med JT - Case reports in vascular medicine JID - 101585357 PMC - PMC13711098 OTO - NOTNLM OT - cervical artery dissection OT - headbanging OT - internal carotid artery dissection OT - ischemic stroke OT - minor trauma COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/27 CRDT- 2026/10/28 05:25 PHST- 2026/07/24 00:00 [received] PHST- 2026/09/10 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:25 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - CRVM9589977 [pii] AID - 10.1155/crvm/9589977 [doi] PST - epublish SO - Case Rep Vasc Med. 2026 Oct 27;2026:9589977. doi: 10.1155/crvm/9589977. eCollection 2026. PMID- 42948317 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2234-943X (Print) IS - 2234-943X (Electronic) IS - 2234-943X (Linking) VI - 16 DP - 2026 TI - Cancer-associated fibroblasts in triple-negative breast cancer: heterogeneity, tumor-stroma crosstalk, and therapeutic resistance. PG - 1977793 LID - 10.3389/fonc.2026.1977793 [doi] LID - 1977793 AB - Triple-negative breast cancer (TNBC) comprises a clinically and biologically heterogeneous group of breast cancers defined by the absence of estrogen receptor and progesterone receptor expression and the lack of human epidermal growth factor receptor 2 (HER2) overexpression or amplification. Although systemic treatment has expanded beyond chemotherapy to include immune checkpoint blockade, poly(ADP-ribose) polymerase (PARP) inhibition in selected patients with germline BRCA1/2 variants, and antibody-drug conjugates in advanced disease, therapeutic resistance and disease recurrence remain major challenges. Cancer-associated fibroblasts (CAFs) are abundant stromal cells that contribute to the organization and function of the TNBC microenvironment. Rather than representing a single uniformly tumor-promoting population, CAFs encompass transcriptionally and functionally diverse states that vary across tumors, spatial niches, and treatment contexts. Current evidence supports roles for selected CAF populations in extracellular matrix (ECM) remodeling, paracrine signaling, metabolic exchange, immune-cell positioning, and adaptation to therapeutic stress. Recent single-cell and spatial studies have further identified ECM-rich, inflammatory, hypoxia-associated, and progenitor-like fibroblast states that correlate with distinct immune landscapes and treatment responses. However, many proposed CAF markers are shared across states, nomenclature remains inconsistent among datasets, and most CAF-directed therapeutic strategies are supported primarily by preclinical evidence. This review summarizes the current understanding of CAF heterogeneity in TNBC, examines the mechanisms through which fibroblast states influence tumor progression and antitumor immunity, and critically evaluates their contribution to chemotherapy and immunotherapy resistance. Particular attention is given to the distinction between mechanistic evidence and associative transcriptomic findings, as well as to the translational challenges that must be addressed before CAF-based biomarkers or therapies can be incorporated into routine TNBC management. CI - Copyright (c) 2026 Wei, Xie, Cao and Wei. FAU - Wei, Shijie AU - Wei S AD - Oncology Center III Department, Qingdao Hiser Hospital Affiliated of Qingdao University, Qingdao Traditional Chinese Medicine Hospital, Qingdao, China. FAU - Xie, Xiaoqing AU - Xie X AD - Oncology Center II Department, Qingdao Hiser Hospital Affiliated of Qingdao University, Qingdao Traditional Chinese Medicine Hospital, Qingdao, China. FAU - Cao, Yufeng AU - Cao Y AD - Oncology Center III Department, Qingdao Hiser Hospital Affiliated of Qingdao University, Qingdao Traditional Chinese Medicine Hospital, Qingdao, China. FAU - Wei, Lingbo AU - Wei L AD - Department of Geriatrics, Qingdao Hiser Hospital Affiliated of Qingdao University, Qingdao Traditional Chinese Medicine Hospital, Qingdao, China. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Oncol JT - Frontiers in oncology JID - 101568867 PMC - PMC13711053 OTO - NOTNLM OT - cancer-associated fibroblasts OT - extracellular matrix OT - immune exclusion OT - single-cell transcriptomics OT - therapy resistance OT - triple-negative breast cancer OT - tumor microenvironment COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:25 PHST- 2026/08/25 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:25 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fonc.2026.1977793 [doi] PST - epublish SO - Front Oncol. 2026 Oct 13;16:1977793. doi: 10.3389/fonc.2026.1977793. eCollection 2026. PMID- 42948315 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-889X (Print) IS - 2296-889X (Electronic) IS - 2296-889X (Linking) VI - 13 DP - 2026 TI - Interpreting antiviral effects: from experimental measurements to mechanistic viral dynamics. PG - 1995001 LID - 10.3389/fmolb.2026.1995001 [doi] LID - 1995001 AB - Antiviral activity is commonly summarized using endpoint measures such as EC50 or EC90, yet these quantities emerge from a dynamic biological system and depend on viral replication kinetics, host-cell properties, experimental design, and the assay used to quantify infection. Infectious-virus titers, viral RNA, infected-cell fractions, viability measurements, and immune readouts capture different components of the infection process and are therefore not interchangeable measures of "viral load." Mechanistic viral-dynamics models provide a framework for linking such observations to underlying processes, including cell infection, intracellular replication, virus production, cell loss, and host-mediated control. However, the biological information that can be recovered from these models is fundamentally constrained by experimental observability and parameter identifiability. This limitation becomes increasingly important across biological scales: in vitro systems permit dense multimodal measurements and controlled perturbations, whereas animal and human studies provide greater physiological relevance but substantially sparser and more indirect observations. Here, we review how experimental readouts map onto viral-dynamics models, how model structure and parameter inference affect mechanistic interpretation, and how controlled in vitro systems can be used to distinguish competing mechanisms of infection and antiviral action. We further discuss examples in which viral-dynamics modeling has contributed to antiviral pharmacology and regimen selection. Together, these considerations support a view of antiviral effects as context-dependent dynamic phenotypes that require joint interpretation of measurements, models, and experimental design. CI - Copyright (c) 2026 Nersisyan. FAU - Nersisyan, Ashot AU - Nersisyan A AD - Faculty of Biology and Biotechnology, HSE University, Moscow, Russia. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Mol Biosci JT - Frontiers in molecular biosciences JID - 101653173 PMC - PMC13711039 OTO - NOTNLM OT - In vitro models OT - antiviral activity OT - antiviral drug development OT - mathematical modeling OT - parameter identifiability OT - pharmacodynamics OT - viral dynamics OT - viral load COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:25 PHST- 2026/09/10 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:25 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 1995001 [pii] AID - 10.3389/fmolb.2026.1995001 [doi] PST - epublish SO - Front Mol Biosci. 2026 Oct 13;13:1995001. doi: 10.3389/fmolb.2026.1995001. eCollection 2026. PMID- 42948308 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1178-7015 (Print) IS - 1178-7015 (Electronic) IS - 1178-7015 (Linking) VI - 19 DP - 2026 TI - Integrated Single-Cell Transcriptomics, Multi-Omics, and Mendelian Randomization Reveal Ferroptosis-Related Molecular Subtypes and a Genetically Supported Causal Candidate in Psoriasis. PG - 637932 LID - 10.2147/CCID.S637932 [doi] LID - 637932 AB - BACKGROUND: Ferroptosis contributes to inflammatory skin diseases, but its role in psoriasis remains incompletely understood. We performed an integrated multi‑omics analysis combining single‑cell transcriptomics, bulk transcriptomics, machine learning, consensus clustering, and Mendelian randomization (MR) to characterize ferroptosis‑related molecular features, intercellular communication, immune microenvironment remodeling, molecular subtypes, and causal biomarkers in psoriasis. METHODS: Single‑cell RNA‑seq data (GSE151177) were analyzed to quantify ferroptosis activity across cell populations and infer intercellular communication networks. Bulk transcriptomic datasets (GSE30999, GSE78097) were integrated for differential expression and machine‑learning feature selection with SHAP interpretation. Consensus clustering, immune infiltration, functional enrichment, and drug repurposing analyses were performed. Cis‑eQTLs from eQTLGen (n=31,684) served as instruments for two‑sample MR with psoriasis GWAS from FinnGen (13,667 cases, 395,092 controls). RESULTS: Macrophages/monocytes and fibroblasts showed the highest ferroptosis scores. MIF signaling (MIF-CD74/CD44, MIF-CD74/CXCR4, MIF-ACKR3) dominated keratinocyte outgoing communication. Transcriptomic integration yielded 72 overlapping ferroptosis-related genes; machine learning identified eight feature genes (CD24, EFNB2, DDX39A, NDRG4, GPRC5A, S100A8, PRSS8, ITPKC). CD24 (AUC=0.979) and S100A8 (AUC=0.974) exhibited top diagnostic performance. Consensus clustering stratified patients into two subtypes (C1, C2); all eight genes were highly expressed in C1, while C2 showed upregulated phagocytic but downregulated innate immune pathways. Drug enrichment suggested MS-275, genistein, and beta-adrenoceptor agonists as potential ferroptosis-modulating compounds. MR demonstrated that only PRSS8 eQTL had a nominally significant causal association with psoriasis risk (P < 0.05, beta = 0.0073). CONCLUSION: Our multi‑omics study delineates the ferroptosis‑related landscape in psoriasis, identifies MIF signaling as a key keratinocyte communication axis, establishes a high‑performance diagnostic model with eight feature genes, defines two distinct immune‑metabolic subtypes, and provides genetic evidence supporting PRSS8 as a putative causal biomarker for psoriasis susceptibility, offering novel insights and potential ferroptosis‑based biomarkers and therapeutic targets for psoriasis. CI - (c) 2026 Xu et al. FAU - Xu, Mengyue AU - Xu M AD - Department of Dermatology, The First Affiliated Hospital of Henan University of Chinese Medicine, Henan Province, People's Republic of China. FAU - Zuo, Yongjie AU - Zuo Y AD - Department of Dermatology, Xinjiang Uygur Autonomous Region Chinese Medicine Hospital, Urumqi, 830000, People's Republic of China. FAU - Liu, Hongxia AU - Liu H AD - Department of Dermatology, Xinjiang Uygur Autonomous Region Chinese Medicine Hospital, Urumqi, 830000, People's Republic of China. FAU - Jia, Huangchao AU - Jia H AD - Department of Dermatology, The First Affiliated Hospital of Henan University of Chinese Medicine, Henan Province, People's Republic of China. FAU - Liu, Xuewei AU - Liu X AD - Department of Dermatology, The First Affiliated Hospital of Henan University of Chinese Medicine, Henan Province, People's Republic of China. AD - Henan University of Chinese Medicine, Zhengzhou, Henan Province, People's Republic of China. LA - eng PT - Journal Article DEP - 20261023 PL - New Zealand TA - Clin Cosmet Investig Dermatol JT - Clinical, cosmetic and investigational dermatology JID - 101543449 PMC - PMC13712149 OTO - NOTNLM OT - Mendelian randomization OT - ferroptosis OT - machine learning OT - molecular subtypes OT - psoriasis OT - single‑cell transcriptomics COIS- The authors report no conflicts of interest in this work. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/23 CRDT- 2026/10/28 05:24 PHST- 2026/07/04 00:00 [received] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:24 [entrez] PHST- 2026/10/23 00:00 [pmc-release] AID - 637932 [pii] AID - 10.2147/CCID.S637932 [doi] PST - epublish SO - Clin Cosmet Investig Dermatol. 2026 Oct 23;19:637932. doi: 10.2147/CCID.S637932. eCollection 2026. PMID- 42948302 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1792-1015 (Electronic) IS - 1792-0981 (Print) IS - 1792-0981 (Linking) VI - 32 IP - 6 DP - 2026 Dec TI - A combination of biopsy using eyeMAX and ERCP for the definitive diagnosis of intraductal papillary mucinous neoplasm: A case report. PG - 337 LID - 10.3892/etm.2026.13332 [doi] LID - 337 AB - As a well-established precursor lesion of pancreatic cancer, intraductal papillary mucinous neoplasm (IPMN) of the pancreas remains characterized by notable challenges with regard to diagnosis and treatment. Owing to its heterogeneous biological behavior, which can range from benign lesions to invasive carcinomas, IPMN represents a marked clinical challenge in management. Biopsy in addition to endoscopic retrograde cholangiopancreatography (ERCP) using eyeMAX can aid in the development of an individualized treatment plan. The present report outlines the case of a patient with suspected IPMN who was admitted to Shandong Provincial Hospital Affiliated to Shandong First Medical University (Jinan, China). Endoscopic ultrasound examination revealed a dilated main pancreatic duct (MPD). The duodenal papilla showed signs of a fish mouth covered with mucus through observation by a duodenoscope. The patient refused the surgical treatment due to concerns regarding the risks of surgery. Subsequently, aided by ERCP using eyeMAX, the villus-like and papillary protrusions were visualized intuitively in the MPD wall of the pancreatic head segment. A total of four pieces of tissue were taken from the pancreatic duct for biopsy, with pathology revealing low-grade dysplasia. Furthermore, direct observation revealed that the wall of the body-tail portion of the MPD was smooth. Given that the pathology revealed a low malignant transformation tendency, it was decided that a follow-up strategy would be conducted for the patient. The most recent follow-up occurred in July 2026. The patient was generally in good condition without jaundice, pancreatitis or abdominal pain. Therefore, biopsies based on ERCP using eyeMAX may be helpful in developing individualized treatment plans for patients with IPMNs in the future. CI - Copyright: (c) 2026 Wang et al. FAU - Wang, Wenwen AU - Wang W AD - Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, P.R. China. FAU - Li, Tao AU - Li T AD - Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, P.R. China. FAU - Hu, Jinhua AU - Hu J AD - Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, P.R. China. FAU - Luan, Zhou AU - Luan Z AD - Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, P.R. China. FAU - Yu, Yuanzi AU - Yu Y AD - Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, P.R. China. LA - eng PT - Case Reports PT - Journal Article DEP - 20261009 PL - Greece TA - Exp Ther Med JT - Experimental and therapeutic medicine JID - 101531947 PMC - PMC13712098 OTO - NOTNLM OT - biopsy OT - endoscopic retrograde cholangiopancreatography OT - eyeMAX OT - intraductal papillary mucinous neoplasm OT - low-grade dysplasia COIS- The authors declare that they have no competing interests. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/09 CRDT- 2026/10/28 05:24 PHST- 2026/02/14 00:00 [received] PHST- 2026/08/04 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:24 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - ETM-32-6-13332 [pii] AID - 10.3892/etm.2026.13332 [doi] PST - epublish SO - Exp Ther Med. 2026 Oct 9;32(6):337. doi: 10.3892/etm.2026.13332. eCollection 2026 Dec. PMID- 42948299 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2214-7500 (Electronic) IS - 2214-7500 (Linking) VI - 16 DP - 2026 Jun TI - Clinical evaluation of oral fluid point-of-care testing for drugs of abuse compared to urinary point-of-care testing at a large-scale music festival. PG - 102192 LID - 10.1016/j.toxrep.2025.102192 [doi] LID - 102192 AB - BACKGROUND: Urinary point-of-care testing for recreational drugs is commonly used in clinical settings. An oral fluid-based point-of-care test is a less invasive alternative, but the reliability and clinical applicability in a real-life acute care setting is unclear. AIM: To assess the concordance of oral fluid point-of-care testing compared to urine point-of-care testing for recreational drugs in a prehospital clinical setting. METHODS: This study was conducted during a large-scale dance music festival in October 2023. Urine and oral fluid samples were collected at the event medical station from volunteers with a suspected drug intoxication. Participants aged 18 years and older were included if both samples were provided and at least one substance tested positive. The percentage of positive oral fluid test results per recreational drug were compared with those of the urine point-of-care test. RESULTS: A total of 78 patients were included. For most drug substances, positivity rates were similar between the two test types. Methamphetamine/3,4-methylenedioxymethamphetamine was the only substance that showed significantly more positive results in oral fluid compared to urine (p < 0.001). For all other substances, the differences between the two tests were small, with slightly higher positivity rates (on average 3.9 %) detected in oral fluid. CONCLUSION: Oral fluid point-of-care testing shows potential in specific scenarios but requires further validation. It is a less invasive alternative to urine point-of-care testing for recreational drugs in a clinical setting. Nevertheless, it is important to consider the differences in test characteristics, such as detection window. Further research is needed to evaluate the reliability in other populations and settings. CI - (c) 2025 The Authors. FAU - Mgm, Frantzen AU - Mgm F AD - Researcher, Department of Hospital Pharmacy, OLVG, Amsterdam, the Netherlands. FAU - Fmj, Gresnigt AU - Fmj G AD - Dutch Poisons Information Center, UMC, Utrecht 3508 GA, the Netherlands. AD - Emergency Department, OLVG Amsterdam, Oosterpark 9, Amsterdam 1091 AC, the Netherlands. FAU - van Rth, Litsenburg AU - van Rth L AD - Event Medical Service, Loopkantstraat 2-E, Uden 5404 NB, the Netherlands. FAU - Ejf, Franssen AU - Ejf F AD - Department of Clinical Pharmacy, OLVG Amsterdam, Oosterpark 9, Amsterdam 1091 AC, the Netherlands. LA - eng PT - Journal Article DEP - 20251218 PL - Ireland TA - Toxicol Rep JT - Toxicology reports JID - 101630272 PMC - PMC13711069 OTO - NOTNLM OT - Drugs of abuse OT - Festival setting OT - Oral fluid drug test OT - Point-of-care testing (POCT) OT - Urine drug test COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2025/12/18 CRDT- 2026/10/28 05:24 PHST- 2025/08/29 00:00 [received] PHST- 2025/12/16 00:00 [revised] PHST- 2025/12/17 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:24 [entrez] PHST- 2025/12/18 00:00 [pmc-release] AID - S2214-7500(25)00311-7 [pii] AID - 102192 [pii] AID - 10.1016/j.toxrep.2025.102192 [doi] PST - epublish SO - Toxicol Rep. 2025 Dec 18;16:102192. doi: 10.1016/j.toxrep.2025.102192. eCollection 2026 Jun. PMID- 42948294 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2976-5390 (Electronic) IS - 2976-5390 (Linking) VI - 4 IP - 10 DP - 2026 Oct TI - SNAP food purchase restrictions and retailer availability: a difference-in-differences analysis. PG - qxag275 LID - 10.1093/haschl/qxag275 [doi] LID - qxag275 AB - INTRODUCTION: States have begun implementing waivers that prohibit the Supplemental Nutrition Assistance Program (SNAP) benefits from being used to purchase selected foods and beverages. We assessed whether these restrictions were associated with changes in the availability of SNAP-authorized convenience stores, supermarkets, and superstores. METHODS: We analyzed repeated observations from 83 697 Census tracts from January 2025 through June 2026. Difference-in-differences models compared changes in retailer availability in states implementing waivers with concurrent changes in states without implemented waivers. RESULTS: Waiver implementation was associated with a 1.4% decline in SNAP-authorized convenience stores, equivalent to 3.0 fewer stores per 100 tracts (95% CI, -5.7 to -0.4; P = 0.03), but not with a significant change in supermarkets or superstores. The largest declines in convenience stores occurred among states restricting beverages and candy (-2.2%; -4.9 stores per 100 tracts; 95% CI, -6.9 to -2.9; P < 0.01). CONCLUSION: The SNAP food restrictions were associated with reduced availability of SNAP-authorized convenience stores but not supermarkets or superstores. Continued monitoring is warranted as these policies expand. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of Health Affairs Publishing, LLC. FAU - Chrisinger, Benjamin W AU - Chrisinger BW AUID- ORCID: 0000-0002-1480-6481 AD - Department of Community Health, Tufts University, Medford, MA 02155, United States. AD - Center for Health and Wellbeing, Princeton University, Princeton, NJ 08544, United States. FAU - White, Justin S AU - White JS AUID- ORCID: 0000-0002-3388-9569 AD - Department of Health Law, Policy & Management, Boston University School of Public Health, Boston, MA 02118, United States. LA - eng PT - Journal Article DEP - 20261010 PL - England TA - Health Aff Sch JT - Health affairs scholar JID - 9918627882906676 PMC - PMC13711658 OTO - NOTNLM OT - difference-in-differences OT - food access OT - state policy OT - supplemental nutrition assistance program COIS- Conflicts of interest: Please see ICMJE form(s) for author conflicts of interest. These have been provided as supplementary materials. None declared. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/10 CRDT- 2026/10/28 05:24 PHST- 2026/08/13 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:24 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - qxag275 [pii] AID - 10.1093/haschl/qxag275 [doi] PST - epublish SO - Health Aff Sch. 2026 Oct 10;4(10):qxag275. doi: 10.1093/haschl/qxag275. eCollection 2026 Oct. PMID- 42948291 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2042-8812 (Print) IS - 2042-8812 (Electronic) IS - 2042-8812 (Linking) VI - 2026 IP - 10 DP - 2026 Oct TI - Circumcision under local anesthesia in a selected case with buried penis: a technique using the Alisklamp Nero M. PG - rjag960 LID - 10.1093/jscr/rjag960 [doi] LID - rjag960 AB - Circumcision in patients with buried penis remains controversial because of the risk of recurrent concealment and secondary complications. This report describes a circumcision technique performed under local anesthesia in a selected case of buried penis. The technique preserves the outer preputial skin while excising only the inner preputial mucosa, using the Alisklamp Nero M device to achieve symmetrical mucosal alignment and protect the glans. Deep Buck's fascia-incorporating sutures at the 3- and 9-o'clock positions enhance penile stability. No early postoperative complications were observed. Because this is a single-case technical report without long-term follow-up data, the findings should be interpreted cautiously. CI - (c) The Author(s) 2026. Published by Oxford University Press and JSCR Publishing Ltd. FAU - Boyaci, Lutfi Ihsan AU - Boyaci LI AUID- ORCID: 0000-0002-4373-515X AD - Department of Urology, Beypazari State Hospital, Ministry of Health, Ayvasik Mahallesi, Beypazari, Ankara 06730, Turkey. FAU - Aras, Necip Fazil AU - Aras NF AUID- ORCID: 0000-0003-2344-8409 AD - Department of Pediatric Surgery, Private Practice of Dr. Necip Fazil Aras, Kucukkolpinar, Alacam Cd. No:146/a, 55200 Atakum, Samsun, Turkey. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - J Surg Case Rep JT - Journal of surgical case reports JID - 101560169 PMC - PMC13711084 OTO - NOTNLM OT - Alisklamp OT - buried penis OT - circumcision COIS- The authors declare that they have no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/27 CRDT- 2026/10/28 05:23 PHST- 2026/09/03 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:23 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - rjag960 [pii] AID - 10.1093/jscr/rjag960 [doi] PST - epublish SO - J Surg Case Rep. 2026 Oct 27;2026(10):rjag960. doi: 10.1093/jscr/rjag960. eCollection 2026 Oct. PMID- 42948290 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2662-6810 (Print) IS - 2052-7276 (Electronic) IS - 2052-7276 (Linking) VI - 13 IP - 11 DP - 2026 Nov TI - Large-scale identification and evolutionary analysis of ATP-binding cassette transporters in cross-lineage plant species. PG - uhag209 LID - 10.1093/hr/uhag209 [doi] LID - uhag209 AB - The ATP-binding cassette (ABC) gene family plays a crucial role in plants by mediating the transport of various molecules, contributing to development, stress responses, and secondary metabolism. Here, 159077ABC genes were identified from 1108 plant species. Comparative analysis revealed that higher plants had more ABC genes compared to algae plants. The ABCG1, ABCG4, and ABCG8 subfamilies originated in land plants and subsequently underwent distinct evolutionary pathways. Motif 1 and motif 9 demonstrated a conserved high-frequency pattern across different lineages. The expansion of ABC family mainly occurred through dispersed and whole-genome duplication in higher angiosperms, and full-length transposons may mediate the neofunctionalization of these genes. The pan-ABC family analysis of 12 tomato accessions revealed more relaxed selective pressures in dispensable ABC genes. The ABC genes of Arabidopsis thaliana exhibited stronger responsiveness and connectivity degree under salt stress. To facilitate further research, we established the PlantABC database (http://biodb.com.cn/PlantABC/index.html), which contains comprehensive genomic and evolutionary information about plant ABC genes. This study provides valuable perspective and resources for advancing research on the evolution and functions of plant ABC genes. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Nanjing Agricultural University. FAU - Song, Kangkang AU - Song K AD - College of Plant Protection and Agricultural Big-Data Research Center, Shandong Agricultural University, Taian 271018, China. FAU - Zhao, Yinuo AU - Zhao Y AD - College of Plant Protection and Agricultural Big-Data Research Center, Shandong Agricultural University, Taian 271018, China. FAU - Xu, He AU - Xu H AD - College of Plant Protection and Agricultural Big-Data Research Center, Shandong Agricultural University, Taian 271018, China. FAU - Li, Haozhen AU - Li H AD - College of Plant Protection and Agricultural Big-Data Research Center, Shandong Agricultural University, Taian 271018, China. FAU - Zhang, Xiaohua AU - Zhang X AD - College of Plant Protection and Agricultural Big-Data Research Center, Shandong Agricultural University, Taian 271018, China. FAU - Song, Yanru AU - Song Y AD - College of Plant Protection and Agricultural Big-Data Research Center, Shandong Agricultural University, Taian 271018, China. FAU - Liu, Boyuan AU - Liu B AD - College of Plant Protection and Agricultural Big-Data Research Center, Shandong Agricultural University, Taian 271018, China. FAU - Wang, Jingyu AU - Wang J AD - College of Plant Protection and Agricultural Big-Data Research Center, Shandong Agricultural University, Taian 271018, China. FAU - Tan, Bin AU - Tan B AD - College of Plant Protection and Agricultural Big-Data Research Center, Shandong Agricultural University, Taian 271018, China. FAU - Zhang, Zhe AU - Zhang Z AD - School of Materials Science and Engineering, Shanghai Institute of Technology, Shanghai 201418, China. FAU - Li, Bin AU - Li B AUID- ORCID: 0009-0005-0567-1553 AD - The Key Laboratory of Plant Development and Environmental Adaptation Biology, Ministry of Education, School of Life Sciences, Shandong University, Qingdao 266237, China. FAU - Liu, Wenjing AU - Liu W AD - College of Plant Protection and Agricultural Big-Data Research Center, Shandong Agricultural University, Taian 271018, China. FAU - Qi, Yiying AU - Qi Y AD - College of Plant Protection and Agricultural Big-Data Research Center, Shandong Agricultural University, Taian 271018, China. FAU - Yang, Long AU - Yang L AUID- ORCID: 0000-0002-9990-8398 AD - College of Plant Protection and Agricultural Big-Data Research Center, Shandong Agricultural University, Taian 271018, China. LA - eng PT - Journal Article DEP - 20260601 PL - England TA - Hortic Res JT - Horticulture research JID - 101655540 PMC - PMC13711656 COIS- The authors declare that they have no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/06/01 CRDT- 2026/10/28 05:23 PHST- 2025/12/17 00:00 [received] PHST- 2026/05/23 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:23 [entrez] PHST- 2026/06/01 00:00 [pmc-release] AID - uhag209 [pii] AID - 10.1093/hr/uhag209 [doi] PST - epublish SO - Hortic Res. 2026 Jun 1;13(11):uhag209. doi: 10.1093/hr/uhag209. eCollection 2026 Nov. PMID- 42948285 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-875X (Print) IS - 2296-875X (Electronic) IS - 2296-875X (Linking) VI - 13 DP - 2026 TI - Prophylactic closed-incision negative pressure wound therapy for open ventral incisional hernia repair with mesh: a meta-analysis of randomized controlled trials. PG - 1962267 LID - 10.3389/fsurg.2026.1962267 [doi] LID - 1962267 AB - OBJECTIVE: To systematically evaluate the effects of prophylactic closed-incision negative pressure wound therapy (ciNPWT) on surgical site infection (SSI), seroma incidence and length of hospital stay after open ventral incisional hernia repair with mesh. METHODS: Four databases (Cochrane Library, Embase, PubMed, Web of Science) were searched from inception to June 2026 for randomized controlled trials (RCTs) comparing prophylactic ciNPWT versus conventional sterile dressings in open ventral incisional hernia repair with mesh. The primary outcome was postoperative SSI incidence; secondary outcomes included seroma incidence and hospital stay. Study quality was assessed using the Cochrane Risk of Bias tool (RoB 1.0), and meta-analysis was performed with both fixed-effect and random-effects models using RevMan software. The certainty of evidence was assessed using the GRADE approach. RESULTS: Four RCTs involving 420 patients (208 in ciNPWT group, 212 in control group) were included. Under the fixed-effect model, ciNPWT tended to reduce SSI risk without statistical significance (RR = 0.52, 95% CI 0.26-1.04, P = 0.06, I(2) = 50%). The random-effects model yielded a consistent but more conservative estimate (RR = 0.55, 95% CI 0.20-1.53, P = 0.25). No significant difference was found in seroma incidence (RR = 0.83, 95% CI 0.39-1.74, P = 0.61). ciNPWT was associated with shortened postoperative hospital stay (MD = -1.02 days, 95% CI -1.61 to -0.43, P = 0.0007), though this finding was based on only two studies and driven primarily by a single trial. Sensitivity analysis indicated that the SSI result was materially influenced by a single trial. Given the small number of included studies (k = 4), funnel plot asymmetry could not be reliably interpreted as evidence of publication bias. The GRADE certainty of evidence was rated as low for SSI and very low for seroma and hospital stay. CONCLUSION: Current evidence from randomized controlled trials suggests that prophylactic ciNPWT may be associated with shortened hospital stay after mesh-based ventral incisional hernia repair and shows a non-significant trend toward reducing SSI and seroma. However, the certainty of evidence is low to very low, the SSI result is materially dependent on a single trial, and the hospital stay finding is inconsistent across studies. Therefore, no definitive conclusion can be drawn regarding infection reduction, and the hospital stay benefit requires confirmation. More large-sample, multicenter, high-quality RCTs are needed for further validation. CI - (c) 2026 Liu, Lu, Li, Li and Li. FAU - Liu, Yihang AU - Liu Y AD - Department of General Surgery, Kaifeng155 Hospital, China RongTong Medical Healthcare Group Co.Ltd., Kaifeng, China. FAU - Lu, Yuqing AU - Lu Y AD - Department of Gastroenterology, Huaihe Hospital, Henan University, Kaifeng, Henan, China. FAU - Li, Song AU - Li S AD - Department of Urology, Kaifeng155 Hospital, China RongTong Medical Healthcare Group Co.Ltd., Kaifeng, China. AD - Kaifeng Minimally Invasive Diagnosis and Treatment Engineering Technology Research Center for Prostate Hyperplasia, Kaifeng, China. AD - Henan Province Prostate Disease Precision Diagnosis and Treatment Engineering Research Center of Traditional Chinese and Western Medicine, Kaifeng, China. AD - Discipline Construction Department, China RongTong Medical Healthcare Group Co.Ltd., Chengdu, China. FAU - Li, Kaichun AU - Li K AD - Department of General Surgery, Kaifeng155 Hospital, China RongTong Medical Healthcare Group Co.Ltd., Kaifeng, China. FAU - Li, Ming AU - Li M AD - Department of General Surgery, Kaifeng155 Hospital, China RongTong Medical Healthcare Group Co.Ltd., Kaifeng, China. LA - eng PT - Journal Article PT - Systematic Review DEP - 20261019 PL - Switzerland TA - Front Surg JT - Frontiers in surgery JID - 101645127 PMC - PMC13711038 OTO - NOTNLM OT - closed-incision negative pressure wound therapy OT - hernia repair OT - meta-analysis OT - surgical site infection OT - ventral incisional hernia COIS- Author(s) YiL, KL, SL and ML were employed by China RongTong Medical Healthcare Group Co.Ltd. The remaining author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/19 CRDT- 2026/10/28 05:23 PHST- 2026/08/08 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:23 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - 10.3389/fsurg.2026.1962267 [doi] PST - epublish SO - Front Surg. 2026 Oct 19;13:1962267. doi: 10.3389/fsurg.2026.1962267. eCollection 2026. PMID- 42948284 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2752-6542 (Electronic) IS - 2752-6542 (Linking) VI - 5 IP - 10 DP - 2026 Oct TI - State-level pathogen prevalence is associated with fewer interracial marriages and more racially restrictive dating preferences. PG - pgag356 LID - 10.1093/pnasnexus/pgag356 [doi] LID - pgag356 AB - The behavioral immune system framework has linked higher levels of environmental pathogens with increased racial prejudice and xenophobia, suggesting that avoidance of racial outgroups may be an evolved strategy to avoid infection by pathogens and parasites. Although previous research has found a correlation between pathogen prevalence and racial bias on both regional and individual levels, few studies have examined whether this pattern predicts real-world outcomes. This paper presents two studies linking ecological pathogen prevalence to US state-level variation in interracial marriage (Study 1) and stated racial preferences on an online dating site (Study 2). States with higher pathogen levels tended to have lower-than-expected rates of interracial marriage, and online daters from these states were more likely to indicate more restrictive racial preferences. These results provide support for the pathogen prevalence hypothesis of prejudice and out-group bias by linking environmental pathogen levels to race-based preferences in marriage and dating. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of National Academy of Sciences. FAU - Freeman, Jason D AU - Freeman JD AUID- ORCID: 0000-0002-9010-2072 AD - Department of Psychological Sciences, William & Mary, Williamsburg, VA 23187, USA. AD - School of Psychology, University of Sussex, Falmer, Brighton BN1 9RH, United Kingdom. FAU - Kusano, Kodai AU - Kusano K AUID- ORCID: 0000-0002-1919-9609 AD - Department of Psychology, New York University Abu Dhabi, Saadiyat Island, Abu Dhabi, UAE. FAU - Schug, Joanna AU - Schug J AUID- ORCID: 0000-0003-1925-9496 AD - Department of Psychological Sciences, William & Mary, Williamsburg, VA 23187, USA. LA - eng PT - Journal Article DEP - 20261017 PL - England TA - PNAS Nexus JT - PNAS nexus JID - 9918367777906676 PMC - PMC13712138 OTO - NOTNLM OT - endogamy OT - interracial relationships OT - mate selection OT - pathogen prevalence OT - racial bias COIS- No competing interests to disclose. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/17 CRDT- 2026/10/28 05:23 PHST- 2026/05/20 00:00 [received] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:23 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - pgag356 [pii] AID - 10.1093/pnasnexus/pgag356 [doi] PST - epublish SO - PNAS Nexus. 2026 Oct 17;5(10):pgag356. doi: 10.1093/pnasnexus/pgag356. eCollection 2026 Oct. PMID- 42948283 OWN - NLM STAT- Publisher LR - 20261029 IS - 2766-2098 (Electronic) IS - 2766-8509 (Print) IS - 2766-2098 (Linking) DP - 2026 Oct 27 TI - High-Performance and Reversible Bitter Taste Sensing Using Aptamer-Functionalized Graphene Channel for Real-Time Electronic Tongue. PG - 70232 LID - 10.1002/exp2.70232 [doi] LID - 70232 AB - Dynamic and real-time sensing of taste is increasingly important for food safety and pharmaceutical quality control, yet the development of advanced sensors for each taste, especially those for bitterness, remains limited. The challenge has been lying on developing suitable receptors for bitter compounds that are capable of reversible operation. Here, we present a graphene-based taste sensor that integrates a quinine-specific aptamer, enabling selective molecular recognition with high sensitivity and operational stability. Especially, unlike previous sensors, which were irreversible or required regeneration steps, our design achieves fully reversible quinine-specific capture and release. Combined with previously developed taste sensors by the authors, graphene-based electronic tongue was demonstrated to differentiate four different taste compounds and even complex beverages. This work highlights the strong potential of the graphene-based sensors with aptamer receptors as reliable and tunable platforms for advanced taste sensing and real-time analysis. CI - (c) 2026 The Author(s). Exploration published by Henan University and John Wiley & Sons Australia, Ltd. FAU - Kim, Hyuk Jin AU - Kim HJ AD - Department of Materials Science and Engineering, Research Institute of Advanced Materials Seoul National University Seoul Republic of Korea. FAU - Hwang, Haesoo AU - Hwang H AD - Department of Materials Science and Engineering, Research Institute of Advanced Materials Seoul National University Seoul Republic of Korea. FAU - Hyun, Jun Uh AU - Hyun JU AD - Department of Materials Science and Engineering, Research Institute of Advanced Materials Seoul National University Seoul Republic of Korea. FAU - Park, Sungwoo AU - Park S AD - Department of Materials Science and Engineering, Research Institute of Advanced Materials Seoul National University Seoul Republic of Korea. FAU - Park, Sohyeon AU - Park S AD - Department of Materials Science and Engineering, Research Institute of Advanced Materials Seoul National University Seoul Republic of Korea. FAU - Kim, Yeong Jae AU - Kim YJ AD - Department of Materials Science and Engineering, Research Institute of Advanced Materials Seoul National University Seoul Republic of Korea. FAU - Lee, Hyeon-Ji AU - Lee HJ AD - Department of Materials Science and Engineering, Research Institute of Advanced Materials Seoul National University Seoul Republic of Korea. FAU - Kwon, Hee Ryeong AU - Kwon HR AD - Department of Materials Science and Engineering, Research Institute of Advanced Materials Seoul National University Seoul Republic of Korea. FAU - Park, Seon Ju AU - Park SJ AD - Department of Materials Science and Engineering, Research Institute of Advanced Materials Seoul National University Seoul Republic of Korea. FAU - Nam, Gi Baek AU - Nam GB AD - Department of Materials Science and Engineering, Research Institute of Advanced Materials Seoul National University Seoul Republic of Korea. FAU - Choe, Min Gyeong AU - Choe MG AD - Department of Materials Science and Engineering, Research Institute of Advanced Materials Seoul National University Seoul Republic of Korea. FAU - Kim, Byungsoo AU - Kim B AD - Department of Materials Science and Engineering, Research Institute of Advanced Materials Seoul National University Seoul Republic of Korea. AD - Department of Electrical and Computer Engineering University of Virginia Charlottesville Virginia USA. FAU - Cho, Soo-Yeon AU - Cho SY AD - School of Chemical Engineering Sungkyunkwan University (SKKU) Suwon Republic of Korea. FAU - Choi, Je Oh AU - Choi JO AD - Department of Materials Science and Engineering Pohang University of Science and Technology (POSTECH) Pohang Republic of Korea. FAU - Kang, Jong-Hoon AU - Kang JH AD - Department of Materials Science and Engineering Pohang University of Science and Technology (POSTECH) Pohang Republic of Korea. FAU - Kim, Dong Sung AU - Kim DS AD - Department of Mechanical Engineering Pohang University of Science and Technology (POSTECH) Pohang Republic of Korea. FAU - Suh, Jun Min AU - Suh JM AD - Department of Materials Science and Engineering, Research Institute of Advanced Materials Seoul National University Seoul Republic of Korea. AD - School of Transdisciplinary Innovations Seoul National University Seoul Republic of Korea. FAU - Kim, Youngeun AU - Kim Y AD - Department of Materials Science and Engineering, Research Institute of Advanced Materials Seoul National University Seoul Republic of Korea. FAU - Jang, Ho Won AU - Jang HW AD - Department of Materials Science and Engineering, Research Institute of Advanced Materials Seoul National University Seoul Republic of Korea. AD - Advanced Institute of Convergence Technology Seoul National University Suwon Republic of Korea. LA - eng PT - Journal Article DEP - 20261027 PL - China TA - Exploration (Beijing) JT - Exploration (Beijing, China) JID - 9918383883006676 PMC - PMC13710939 OTO - NOTNLM OT - aptamer OT - field-effect transistor OT - graphene OT - quinine OT - taste sensor COIS- Ho Won Jang is a member of the Exploration editorial board, and he was not involved in the handling or peer review process of this manuscript. The authors declare no conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/27 CRDT- 2026/10/28 05:23 PHST- 2025/11/19 00:00 [received] PHST- 2026/02/11 00:00 [revised] PHST- 2026/06/02 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:23 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - EXP270232 [pii] AID - 10.1002/exp2.70232 [doi] PST - aheadofprint SO - Exploration (Beijing). 2026 Oct 27:70232. doi: 10.1002/exp2.70232. PMID- 42948280 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2048-7177 (Print) IS - 2048-7177 (Electronic) IS - 2048-7177 (Linking) VI - 14 IP - 11 DP - 2026 Nov TI - Harvest Time Influences Inulin and Phenolic Accumulation of Globe Artichoke (Cynara cardunculus L.). PG - e72490 LID - 10.1002/fsn3.72490 [doi] LID - e72490 AB - The influence of harvest time on globe artichoke (Cynara cardunculus L.) inulin and phenolics was investigated to inform selection of superior lines with improved nutritional quality. Lines of unknown genetics were harvested early, middle or late in the growing season. Inflorescence size and edible portion (EP) yield, fructan content, including inulin-type fructans, inulin profile, phenolic concentrations, color change (browning) after cutting and polyphenol oxidase activity were measured. Early harvested artichokes developed larger inflorescences (average 248.3 g) compared to late harvested artichokes (average 178.5 g) and had higher mean total phenolic concentrations (TPC) (8.67 g kg(-1)) in the EP. Late harvest artichokes had higher mean fructan concentrations (61.56 g kg(-1) fresh weight) and higher mean yield of EP (54.9%) compared to early harvest artichokes (36.1%). Inulin concentration was positively correlated with mean daily solar radiation exposure, while phenolic concentration was negatively correlated. The CIE b* value (yellowness) of the artichoke receptacle immediately after cutting was significantly, positively correlated with the TPC, chlorogenic acid, and dicaffeoylquinic acid isomer (DiCQA) concentrations. The rate and intensity of polyphenol oxidase-mediated browning after cutting was correlated with phenolic concentrations, with DiCQA having the strongest association. This research demonstrates the potential benefits of evaluating a wide range of variables to select superior artichoke lines with improved nutritional quality. CI - (c) 2026 The Author(s). Food Science & Nutrition published by Wiley Periodicals LLC. FAU - Tilley, Andrew AU - Tilley A AUID- ORCID: 0000-0002-5143-3549 AD - Centre for Crop and Food Innovation, Food Futures Institute Murdoch University Perth Western Australia Australia. AD - School of Medical, Molecular and Forensic Sciences Murdoch University Perth Western Australia Australia. FAU - McHenry, Mark P AU - McHenry MP AD - School of Agricultural Sciences Murdoch University Perth Western Australia Australia. AD - Mt Lindesay Scotsdale Western Australia Australia. FAU - McHenry, Julia Anwar AU - McHenry JA AUID- ORCID: 0000-0002-3983-4622 AD - Mt Lindesay Scotsdale Western Australia Australia. FAU - Wang, Yimin AU - Wang Y AUID- ORCID: 0009-0009-6888-5726 AD - Australian National Phenome Centre, Health Futures Institute Murdoch University Perth Western Australia Australia. FAU - Shemesavar, Soudabeh AU - Shemesavar S AUID- ORCID: 0000-0002-8742-9357 AD - School of Mathematics, Statistics, Chemistry and Physics Murdoch University Perth Western Australia Australia. FAU - Kowalczyk, Malgorzata AU - Kowalczyk M AD - School of Mathematics, Statistics, Chemistry and Physics Murdoch University Perth Western Australia Australia. FAU - Loo, Ruey Leng AU - Loo RL AUID- ORCID: 0000-0001-5307-5709 AD - Australian National Phenome Centre, Health Futures Institute Murdoch University Perth Western Australia Australia. AD - Centre for Computational and Systems Medicine, Health Futures Institute Murdoch University Perth Western Australia Australia. FAU - Bayliss, Kirsty L AU - Bayliss KL AUID- ORCID: 0000-0002-4431-3389 AD - Centre for Crop and Food Innovation, Food Futures Institute Murdoch University Perth Western Australia Australia. AD - School of Agricultural Sciences Murdoch University Perth Western Australia Australia. FAU - Solah, Vicky A AU - Solah VA AD - Centre for Crop and Food Innovation, Food Futures Institute Murdoch University Perth Western Australia Australia. AD - School of Medical, Molecular and Forensic Sciences Murdoch University Perth Western Australia Australia. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Food Sci Nutr JT - Food science & nutrition JID - 101605473 PMC - PMC13711686 OTO - NOTNLM OT - browning OT - chlorogenic acid OT - color OT - dicaffeoylquinic acid OT - polyphenol COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/27 CRDT- 2026/10/28 05:22 PHST- 2026/04/23 00:00 [received] PHST- 2026/08/25 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:22 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - FSN372490 [pii] AID - 10.1002/fsn3.72490 [doi] PST - epublish SO - Food Sci Nutr. 2026 Oct 27;14(11):e72490. doi: 10.1002/fsn3.72490. eCollection 2026 Nov. PMID- 42948279 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2168-8184 (Print) IS - 2168-8184 (Electronic) IS - 2168-8184 (Linking) VI - 18 IP - 9 DP - 2026 Sep TI - Large, Life-Threatening Right Ventricular and Pulmonary Artery Thrombus Revealing Antiphospholipid Syndrome: The Role of Risk Stratification and Thrombolytic Therapy. PG - e116979 LID - 10.7759/cureus.116979 [doi] LID - e116979 AB - Right ventricular thrombus in the setting of acute pulmonary embolism is an uncommon and potentially life-threatening finding associated with a high risk of hemodynamic deterioration. The optimal management remains controversial, particularly in patients with an extensive thrombotic burden but without overt hemodynamic instability. We report a case of a patient presenting with an extensive thrombus occupying the right ventricle and extending into the pulmonary artery. Multimodality imaging demonstrated a significant thrombotic burden with right ventricular involvement and confirmed acute pulmonary embolism. Echocardiographic assessment revealed right ventricular thrombus and signs of right ventricular pressure overload. Based on the clinical presentation, imaging findings, and right ventricular involvement, the patient was classified as having intermediate-high-risk pulmonary embolism. Given the large mobile right ventricular thrombus with pulmonary arterial extension, profound hypoxemia (oxygen saturation: 54%) on room air, and severe tachypnea (45 breaths/min), systemic thrombolysis was considered despite the absence of hemodynamic collapse. After multidisciplinary discussion, an individualized treatment decision was made to administer reduced-dose tenecteplase (0.25 mg/kg; 15 mg). Marked clinical improvement was observed within 24 hours, with no major bleeding complications. Further investigations revealed antiphospholipid syndrome as the underlying prothrombotic condition. At three-month follow-up, the patient remained clinically stable, with a marked reduction in the residual right ventricular thrombus and resolution of the pulmonary trunk thrombus. This case highlights the importance of early echocardiographic identification of right ventricular thrombus in patients with pulmonary embolism and the role of multimodality imaging in therapeutic decision-making. It also illustrates the potential role of reduced-dose systemic thrombolysis in selected patients with intermediate-high-risk pulmonary embolism and extensive intracardiac thrombotic burden. Recognition of the underlying prothrombotic disorder is essential for guiding long-term anticoagulation and preventing recurrent thromboembolic events. CI - Copyright (c) 2026, Seghrouchni et al. FAU - Seghrouchni, Hamza AU - Seghrouchni H AD - Cardiology A Department, Centre Hospitalier Ibn Sina, Rabat, MAR. FAU - Boubekri, Zineb AU - Boubekri Z AD - Cardiology A Department, Centre Hospitalier Ibn Sina, Rabat, MAR. FAU - Haouani, Hiba AU - Haouani H AD - Cardiology A Department, Centre Hospitalier Ibn Sina, Rabat, MAR. FAU - Achour, Fadoua AU - Achour F AD - Cardiology A Department, Centre Hospitalier Ibn Sina, Rabat, MAR. FAU - Nadhil, Zayna AU - Nadhil Z AD - Cardiology A Department, Centre Hospitalier Ibn Sina, Rabat, MAR. FAU - Fellat, Nadia AU - Fellat N AD - Cardiology A Department, Centre Hospitalier Ibn Sina, Rabat, MAR. AD - Cardiology, Faculty of Medicine and Pharmacy of Rabat, Rabat, MAR. LA - eng PT - Case Reports PT - Journal Article DEP - 20260926 PL - United States TA - Cureus JT - Cureus JID - 101596737 PMC - PMC13710945 OTO - NOTNLM OT - antiphospholipid syndrome OT - pulmonary embolism OT - right ventricular thrombus OT - tenecteplase OT - thrombolysis COIS- Human subjects: Informed consent for treatment and open access publication was obtained or waived by all participants in this study. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/09/26 CRDT- 2026/10/28 05:22 PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:22 [entrez] PHST- 2026/09/26 00:00 [pmc-release] AID - 10.7759/cureus.116979 [doi] PST - epublish SO - Cureus. 2026 Sep 26;18(9):e116979. doi: 10.7759/cureus.116979. eCollection 2026 Sep. PMID- 42948278 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1752-4571 (Print) IS - 1752-4571 (Electronic) IS - 1752-4571 (Linking) VI - 19 IP - 10 DP - 2026 Oct TI - Cancer Evolvability Determines Therapy Outcomes. PG - e70320 LID - 10.1111/eva.70320 [doi] LID - e70320 AB - Cancer progression following treatment failure is an evolutionary process in which therapy acts as a selection pressure driving Darwinian selection on heritable variation to favor resistant clones. Heritable variation is a key determinant of how rapidly tumors can adapt to therapy. Heritable variation arises through various mechanisms such as genetic mutations, epigenetics, chromosomal rearrangements, and copy number variations, resulting in a lack of replicative fidelity. We consider evolvability as a property or trait of the cancer cells that influences the rate at which fitness-relevant heritable variation is generated. Using this as our definition, we present an evolutionary game-theoretic model to evaluate how evolvability shapes resistance dynamics under two treatment modalities: targeted therapy and chemotherapy. We first compare cancer populations with fixed evolvabilities: low or high. Targeted therapy imposes a steep selection gradient, enabling rapid resistance evolution, while chemotherapy imposes a flatter selection gradient but drives tumors toward more extreme resistance strategies. We show that targeted therapy works better in cancers with low evolvability, whereas chemotherapy better controls cancers with high evolvability. We then extend the model to incorporate "plastic evolvability," in which cancer cells can adjust their evolvability in response to therapy-induced stress. In this case, cells fine-tune the trade-off between acquiring higher resistance and limiting the costs of resistance and evolvability. Plastic evolvability sustains a higher tumor burden than fixed-evolvability. To address the challenge of plastic evolvability, we develop and simulate an evolutionary double bind using sequential cycles of chemotherapy and targeted therapy. With an appropriate sequence and timing, the double bind can drive cancers with plastic evolvability to extinction. Our results highlight the importance of evolvability in shaping treatment response and underscore the need to incorporate evolutionary principles into therapy design. CI - (c) 2026 The Author(s). Evolutionary Applications published by John Wiley & Sons Ltd. FAU - Bhattacharya, Ranjini AU - Bhattacharya R AUID- ORCID: 0000-0002-5045-9333 AD - Department of Integrated Mathematical Oncology H. Lee Moffitt Cancer Center and Research Institute Tampa Florida USA. AD - Department of Cancer Biology University of South Florida Tampa Florida USA. FAU - Bukkuri, Anuraag AU - Bukkuri A AUID- ORCID: 0000-0002-3616-626X AD - Department of Mathematics City St. George's, University of London London UK. FAU - Gatenby, Robert A AU - Gatenby RA AUID- ORCID: 0000-0002-1621-1510 AD - Department of Radiology H. Lee Moffitt Cancer Center and Research Institute Tampa Florida USA. AD - Cancer Biology and Evolution Program Moffitt Cancer Center and Research Institute Tampa Florida USA. FAU - Brown, Joel S AU - Brown JS AUID- ORCID: 0000-0002-0958-9494 AD - Department of Integrated Mathematical Oncology H. Lee Moffitt Cancer Center and Research Institute Tampa Florida USA. AD - Cancer Biology and Evolution Program Moffitt Cancer Center and Research Institute Tampa Florida USA. LA - eng PT - Journal Article DEP - 20261025 PL - England TA - Evol Appl JT - Evolutionary applications JID - 101461828 PMC - PMC13711687 OTO - NOTNLM OT - cancer evolution OT - chemotherapy OT - evolutionary game theory OT - evolutionary medicine OT - evolvability OT - targeted therapy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/25 CRDT- 2026/10/28 05:22 PHST- 2026/05/08 00:00 [received] PHST- 2026/07/19 00:00 [revised] PHST- 2026/08/25 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:22 [entrez] PHST- 2026/10/25 00:00 [pmc-release] AID - EVA70320 [pii] AID - 10.1111/eva.70320 [doi] PST - epublish SO - Evol Appl. 2026 Oct 25;19(10):e70320. doi: 10.1111/eva.70320. eCollection 2026 Oct. PMID- 42948273 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2628-5452 (Electronic) IS - 2628-5452 (Linking) VI - 7 IP - 2 DP - 2026 Dec TI - Recent Advances in Reversed-Phase Liquid Chromatography for Therapeutic Oligonucleotide and Conjugate Characterization: From Ion-Pairing to Green Analytical Approaches. PG - e70113 LID - 10.1002/ansa.70113 [doi] LID - e70113 AB - Therapeutic oligonucleotides (ONs) have rapidly emerged as a novel class of treatments in the pharmaceutical industry, with over 25 medicines approved in the past decade. Ion-pair reversed-phase liquid chromatography coupled with mass spectrometry (IP-RPLC-MS) is widely used method for ON characterization. This review critically examines the state-of-the-art developments in IP-RPLC-MS for ON analysis, with particular focus on the selection of optimal chromatographic conditions for three key applications: impurity profiling for single-stranded ON, characterization of novel conjugates (e.g., lipid-and peptide-conjugated ONs) and double-stranded siRNA analysis in non-denaturing conditions. Finally, recent developments in ion-pair-free RPLC are discussed in the context of pharmaceutical and bioanalytical applications. CI - (c) 2026 The Author(s). Analytical Science Advances published by Wiley-VCH GmbH. FAU - Bui, Quang-Dong AU - Bui QD AUID- ORCID: 0000-0003-4807-727X AD - Department of Chemical Engineering Vrije Universiteit Brussel (VUB) Brussels Belgium. FAU - Hewelt-Belka, Weronika AU - Hewelt-Belka W AD - Faculty of Chemistry Department of Analytical Chemistry Gdansk University of Technology Gdansk Poland. FAU - Deschrijver, Tiny AU - Deschrijver T AD - Therapeutics Development & Supply Janssen Research & Development NV Johnson & Johnson Company Beerse Belgium. FAU - Gilar, Martin AU - Gilar M AUID- ORCID: 0000-0003-1199-1287 AD - Waters Corporation Global Research Milford Massachusetts USA. FAU - Eeltink, Sebastiaan AU - Eeltink S AUID- ORCID: 0000-0002-5465-0127 AD - Department of Chemical Engineering Vrije Universiteit Brussel (VUB) Brussels Belgium. LA - eng PT - Journal Article PT - Review DEP - 20261026 PL - Germany TA - Anal Sci Adv JT - Analytical science advances JID - 9918334986206676 PMC - PMC13710897 OTO - NOTNLM OT - ion-pair free OT - non-denaturing OT - oligonucleotide-conjugate OT - siRNA COIS- Sebastiaan Eeltink is an editor of Analytical Science Advances and co-author of this article. He was excluded from editorial decision-making related to the acceptance of this article for publication in the journal. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/26 CRDT- 2026/10/28 05:22 PHST- 2026/08/03 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:22 [entrez] PHST- 2026/10/26 00:00 [pmc-release] AID - ANSA70113 [pii] AID - 10.1002/ansa.70113 [doi] PST - epublish SO - Anal Sci Adv. 2026 Oct 26;7(2):e70113. doi: 10.1002/ansa.70113. eCollection 2026 Dec. PMID- 42948266 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2168-8184 (Print) IS - 2168-8184 (Electronic) IS - 2168-8184 (Linking) VI - 18 IP - 9 DP - 2026 Sep TI - Comparative Performance of Oxygenation Indices for Mortality Prediction in Patients With Acute Respiratory Distress Syndrome at a Tertiary Care Intensive Care Unit in Bangladesh. PG - e116997 LID - 10.7759/cureus.116997 [doi] LID - e116997 AB - Background Acute respiratory distress syndrome (ARDS) is a syndrome of acute inflammatory lung injury and impaired gas exchange, not hypoxemia alone, and carries high mortality. This study compared the discrimination of the partial pressure of arterial oxygen (PaO(2))/fraction of inspired oxygen (FiO(2)) (PF) ratio, oxygenation index (OI), and oxygen saturation index (OSI), measured on the day of ARDS diagnosis, for intensive care unit (ICU) mortality in a Bangladeshi tertiary ICU. Methods This single-center prospective observational study enrolled 80 consecutive adults meeting the 2012 Berlin definition of ARDS and requiring invasive mechanical ventilation at Bangladesh Medical University between June 2024 and May 2025. Time zero (day 1) was the day the Berlin criteria were first met. Indices were calculated on days 1, 3, and 7 from simultaneous blood gas, oximetry, and ventilator data. Groups were compared by independent t- and chi-square tests, serial change by paired t-tests in survivors only, and discrimination by receiver operating characteristic (ROC) curve analysis with Youden cutoffs. Correlated areas under the curve (AUCs) were not formally compared, and no multivariable model was fitted. Results Forty-five of 80 patients (56.3%) died in the ICU, and 35 (43.8%) survived. Non-survivors had lower blood pressure, higher heart and respiratory rates, and greater ventilatory requirements (p<0.05). OI and OSI were higher and the PF ratio was lower among non-survivors (p<0.05). Discrimination was numerically highest for OSI (AUC=0.879; 95% CI: 0.786-0.977), followed by OI (AUC=0.867; 95% CI: 0.788-0.963) and the PF ratio (AUC=0.731; 95% CI: 0.612-0.881); as the intervals overlapped and no paired test was applied, this ordering is descriptive only. Specificity was 91.4% for both OSI and OI, and accuracy was 86.3% and 83.8% versus 70% for the PF ratio. Serial improvement among survivors was not statistically significant. Conclusions OI and OSI were higher among non-survivors and showed numerically higher discrimination for ICU mortality than the PF ratio, with OSI performing best. As the analysis was univariable and cutoffs were internally derived, these findings indicate discrimination and association rather than independent prognostic value, and the thresholds are exploratory. Larger multicenter studies with formal paired comparison of ROC curves, multivariable adjustment, and independent validation are warranted. CI - Copyright (c) 2026, Naim et al. FAU - Naim, Jannatun AU - Naim J AD - Department of Critical Care Medicine, Ministry of Health and Family Welfare, Dhaka, BGD. FAU - Afroz, Nafiza AU - Afroz N AD - Department of Anesthesia, Intensive Care, and Pain Medicine, National Institute of Burn and Plastic Surgery, Dhaka, BGD. FAU - Sultana, Most Nasrin AU - Sultana MN AD - Department of Critical Care Medicine, Rajshahi Medical College Hospital, Rajshahi, BGD. FAU - Hossain, Sabiha Binte AU - Hossain SB AD - Department of Critical Care Medicine, Central Police Hospital, Dhaka, BGD. FAU - Uddin, A B M Borhan AU - Uddin ABMB AD - Department of Critical Care Medicine, Bangladesh Medical University, Dhaka, BGD. LA - eng PT - Journal Article DEP - 20260927 PL - United States TA - Cureus JT - Cureus JID - 101596737 PMC - PMC13711625 OTO - NOTNLM OT - acute respiratory distress syndrome OT - mortality prediction OT - oxygen saturation index OT - oxygenation index OT - pf ratio COIS- Human subjects: Informed consent for treatment and open access publication was obtained or waived by all participants in this study. Institutional Review Board (IRB) of Bangladesh Medical University issued approval BSMMU/2024/5688. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/09/27 CRDT- 2026/10/28 05:22 PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:22 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - 10.7759/cureus.116997 [doi] PST - epublish SO - Cureus. 2026 Sep 27;18(9):e116997. doi: 10.7759/cureus.116997. eCollection 2026 Sep. PMID- 42948255 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2837-6056 (Electronic) IS - 2837-6056 (Linking) VI - 4 DP - 2026 TI - Motion-corrected structural realignment and quantitative voxel targeting in magnetic resonance spectroscopy. LID - IMAG.a.1425 [pii] LID - 10.1162/IMAG.a.1425 [doi] AB - Magnetic resonance spectroscopy (MRS) non-invasively quantifies neurochemical metabolites within a prescribed voxel in the brain, but is highly sensitive to head motion that displaces the voxel from its intended anatomical location, and introduces spectral contamination as well as tissue estimation errors. In particular, voxel displacement into bone tissue severely degrades spectral quality due to lipid contamination, often rendering the data unusable. While promising solutions exist to address motion-related issues, many rely on specialized infrastructure and expertise available only at a limited number of research centers. To address inter-acquisition motion, we combine automatic voxel repositioning using an updated 18 s head scout MRI, acquired immediately before MRS, with realignment of the structural MRI to ensure that tissue correction is based on the updated voxel location. Furthermore to quantify anatomical targeting accuracy, we introduced a voxel-ROI overlap metric that measures the percentage intersection between the prescribed voxel and atlas-defined target regions, enabling objective evaluation of voxel positioning accuracy. Using voxels prescribed across three brain regions (prefrontal cortex, thalamus, left superior temporal gyrus; N = 31), realignment on average increased voxel-ROI overlap by 24%, reduced inter-subject variability in tissue composition by 8.1%, and eliminated bone contamination in all six affected cases. Our computationally efficient method executes in < 35 s per participant and requires no specialized hardware, offering an accessible solution that integrates inter-acquisition motion correction with quantitative assessment to achieve accurate voxel targeting. CI - (c) 2026 The Authors. Published under a Creative Commons Attribution 4.0 International (CC BY 4.0) license. FAU - Manting, Cassia Low AU - Manting CL AUID- ORCID: 0000-0001-9339-3402 AD - Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States. AD - McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, United States. AD - Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden. FAU - Takahashi, Atsushi AU - Takahashi A AD - McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, United States. FAU - Ahveninen, Jyrki AU - Ahveninen J AUID- ORCID: 0000-0001-9058-8669 AD - Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States. FAU - Mujica-Parodi, Lilianne R AU - Mujica-Parodi LR AUID- ORCID: 0000-0002-3752-5519 AD - Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States. AD - Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY, United States. FAU - Ratai, Eva-Maria AU - Ratai EM AUID- ORCID: 0000-0001-9476-6207 AD - Athinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States. LA - eng PT - Journal Article DEP - 20261026 PL - United States TA - Imaging Neurosci (Camb) JT - Imaging neuroscience (Cambridge, Mass.) JID - 9918663686606676 SB - IM MH - Humans MH - Magnetic Resonance Spectroscopy/methods MH - *Magnetic Resonance Imaging/methods MH - *Brain/diagnostic imaging/metabolism MH - *Image Processing, Computer-Assisted/methods MH - Motion PMC - PMC13710932 OTO - NOTNLM OT - MRS OT - alignment OT - motion OT - motion correction OT - spectroscopy OT - voxel position COIS- The authors declare no competing interests. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/26 CRDT- 2026/10/28 05:21 PHST- 2026/05/21 00:00 [received] PHST- 2026/08/21 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:21 [entrez] PHST- 2026/10/26 00:00 [pmc-release] AID - IMAG.a.1425 [pii] AID - 10.1162/IMAG.a.1425 [doi] PST - epublish SO - Imaging Neurosci (Camb). 2026 Oct 26;4:IMAG.a.1425. doi: 10.1162/IMAG.a.1425. eCollection 2026. PMID- 42948253 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2673-561X (Electronic) IS - 2673-561X (Print) IS - 2673-561X (Linking) VI - 7 DP - 2026 TI - Telemedicine for chronic pain management in rural populations: a narrative review. PG - 1907994 LID - 10.3389/fpain.2026.1907994 [doi] LID - 1907994 AB - Chronic pain is among the most prevalent and burdensome health conditions in the United States, disproportionately affecting rural populations who face compounding barriers to specialty care, including geographic distance, transportation burden, specialist shortages, and limited access to multidisciplinary services. These structural inequities contribute to persistent disparities in pain prevalence, pain-related disability, and opioid utilization. Telemedicine is a promising strategy for reducing this access gap, with adoption accelerating markedly following the COVID-19 pandemic. This narrative review examines telemedicine for chronic pain management in rural populations across synchronous video visits, telephone-based encounters, asynchronous electronic consultations, telerehabilitation, tele-delivered cognitive behavioral therapy, and mobile health applications. The evidence base is strongest for remotely delivered cognitive behavioral therapy and selected telerehabilitation interventions, whereas evidence for specialty video programs, electronic consultations, Veterans Health Administration programs, and mobile health remains more heterogeneous and frequently observational. Telemedicine can improve access, psychological outcomes, functional status, and patient convenience while reducing travel burden, but it does not consistently improve pain intensity and may worsen inequities when broadband, device access, digital literacy, or privacy are inadequate. Important implementation challenges include limitations of the remote physical examination, variable patient preferences, technology anxiety, and regulatory uncertainty governing telemedicine prescribing of controlled substances. A hybrid model that uses structured triage, low-bandwidth alternatives, technology-navigation support, standardized opioid monitoring, and clearly defined triggers for in-person evaluation represents the most pragmatic framework for rural chronic pain care in the U.S. context. CI - (c) 2026 Hasoon, Yazdi and Robinson. FAU - Hasoon, Jamal AU - Hasoon J AD - Department of Anesthesiology, Critical Care and Pain Medicine, The University of Texas Health Science Center at Houston, Houston, TX, United States. FAU - Yazdi, Cyrus AU - Yazdi C AD - Department of Anesthesiology, Critical Care and Pain Medicine, Beth Israel Deaconess Medical Center, Boston, MA, United States. FAU - Robinson, Christopher L AU - Robinson CL AD - Department of Anesthesiology and Critical Care Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD, United States. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Pain Res (Lausanne) JT - Frontiers in pain research (Lausanne, Switzerland) JID - 9918227269806676 PMC - PMC13710922 OTO - NOTNLM OT - chronic pain OT - health disparities OT - pain management OT - rural health OT - telehealth OT - telemedicine COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:21 PHST- 2026/06/13 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:21 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fpain.2026.1907994 [doi] PST - epublish SO - Front Pain Res (Lausanne). 2026 Oct 13;7:1907994. doi: 10.3389/fpain.2026.1907994. eCollection 2026. PMID- 42948250 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2397-1991 (Electronic) IS - 2397-1983 (Print) IS - 2397-1983 (Linking) VI - 11 IP - 2 DP - 2026 TI - Novel clustering approach to understanding distinct phenotypes of patients with systemic sclerosis. PG - e000041 LID - 10.1136/jsrd-2026-000041 [doi] LID - e000041 AB - OBJECTIVES: Despite earlier clustering studies on systemic sclerosis (SSc), how clinical subgroups differ in disease burden, treatment and patient-physician dynamics remains unclear. This study applied a novel clustering method to identify clinical subgroups. DESIGN: Data were analysed from 1906 adults with SSc and their treating rheumatologists across the USA, France, Germany, Italy, Spain, UK and Japan (March-September 2022). SETTING: Data were collected by Adelphi Real World as part of an independent survey titled the SSc I Disease Specific Programme, comprising data from the USA, France, Germany, Italy, Spain, UK and Japan. PARTICIPANTS: Physicians completed case record forms; patients completed self-reported questionnaires. Latent class clustering was conducted using five physician-reported variables: affected body areas, symptoms, severity, flares in the past 12 months and remission status. INTERVENTIONS: This was a non-interventional, observational, retrospective study. MAIN OUTCOME MEASURES: A cluster solution was selected based on model fit and clinical interpretability. We compared time to diagnosis, symptoms at diagnosis, postdiagnosis trajectories, physician management and patient treatment perspectives across clusters. RESULTS: We identified four clusters representing a gradient of disease severity, with all clusters comprising >75% females. Clusters 1/2 comprised younger patients with fewer concomitant conditions; Clusters 3/4 showed greater internal organ involvement and disease severity. Raynaud's phenomenon (RP) was the most frequent first symptom (physician-reported: 73%-85%; patient-reported: 63%-79%), but those with first symptoms other than RP were diagnosed faster (mean 1.06 vs 1.62 years). Musculoskeletal involvement was most pronounced in Clusters 2/4. Postdiagnosis (3.7-7.2 years), the number of symptoms and affected body areas decreased in Cluster 1, increased in Cluster 4 and remained relatively stable in Clusters 2/3. Improvement in disease progression within the first 2 years postdiagnosis was more often reported in Clusters 1/2 vs 3/4 (33%-34% vs 8%-20%). Most patients across clusters reported that the main benefit they wanted from treatment was to get rid of the disease. CONCLUSIONS: This analysis improves understanding of SSc beyond limited/diffuse subtypes. Musculoskeletal involvement emerged as a contributor to SSc burden, supporting its integration into monitoring/stratification strategies for earlier identification of high-risk patients. CI - Copyright (c) Author(s) (or their employer(s)) 2026. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ Group. FAU - Khanna, Dinesh AU - Khanna D AUID- ORCID: 0000-0003-1412-4453 AD - Department of Internal Medicine, University of Michigan Scleroderma Program, Ann Arbor, Michigan, USA. FAU - Ehlers, Mario AU - Ehlers M AD - Therapeutic Area Inflammation, Boehringer Ingelheim Pharmaceuticals, Inc, Ridgefield, Connecticut, USA. FAU - Moros, Lizette AU - Moros L AD - Therapeutic Area Inflammation, Boehringer Ingelheim International GmbH, Ingelheim am Rhein, Germany. FAU - Misra, Arpit AU - Misra A AD - Therapeutic Area Mental and Eye Health, Boehringer Ingelheim International GmbH, Ingelheim am Rhein, Germany. FAU - Song, Hyemin AU - Song H AD - Cross Therapeutic Area Marketing and Operations, Boehringer Ingelheim International GmbH, Ingelheim am Rhein, Germany. FAU - Truman, Isabel AU - Truman I AD - Adelphi Real World, Bollington, UK. FAU - Gillespie-Akar, Liane AU - Gillespie-Akar L AD - Adelphi Real World, Bollington, UK. FAU - Del Galdo, Francesco AU - Del Galdo F AD - University of Leeds, Leeds, UK. LA - eng PT - Journal Article DEP - 20261007 PL - England TA - J Scleroderma Relat Disord JT - Journal of scleroderma and related disorders JID - 101685427 PMC - PMC13711579 OTO - NOTNLM OT - Cluster Analysis OT - Disease Progression OT - Scleroderma, Systemic COIS- DK has been a consultant to AbbVie, Amgen, Argenx, AstraZeneca, Bristol Myers Squibb, Boehringer Ingelheim, Cabaletta, CSL Behring, Galderma, GSK, Janssen, Liquidia, Merck, Mitsubishi Tanabe, Novartis, Pharmosa and Zuro Bio; and has received grants from Amgen, Bayer, Bristol Myers Squibb, Boehringer Ingelheim and Pfizer. ME was an employee of Boehringer Ingelheim at the time this work was conducted. His current affiliation is Autoimmune Clinical Development, Immunocore LLC, Gaithersburg, Maryland, USA. LM, AM and HS are employees of Boehringer Ingelheim. IT and LG-A are employees of Adelphi Real World. FDG declares the following-Speakers' bureau: AstraZeneca and Janssen; Consultant: AstraZeneca, Boehringer Ingelheim, Capella, Chemomab, Janssen and Mitsubishi Tanabe; Grant/research support: AbbVie, AstraZeneca, Boehringer Ingelheim, Capella, Chemomab, Kymab, Janssen and Mitsubishi Tanabe. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/07 CRDT- 2026/10/28 05:21 PHST- 2026/06/18 00:00 [received] PHST- 2026/08/14 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:21 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - jsrd-2026-000041 [pii] AID - 10.1136/jsrd-2026-000041 [doi] PST - epublish SO - J Scleroderma Relat Disord. 2026 Oct 7;11(2):e000041. doi: 10.1136/jsrd-2026-000041. eCollection 2026. PMID- 42948249 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 17 DP - 2026 TI - The double-edged sword of TOX: orchestrating T cell exhaustion and epigenetic remodeling in the tumor microenvironment. PG - 1957165 LID - 10.3389/fimmu.2026.1957165 [doi] LID - 1957165 AB - The capacity to profoundly change chromatin domains and in turn drive or inhibit gene expression makes chromatin modulators a powerful tool adopted to regulate crucial steps during development and cell lineage specification. These proteins can however be as well engaged to irreversibly reprogram cells toward pathological outcomes such as cancer. An example of such chromatin remodelers is the family of Thymocyte-selection associated high mobility box proteins (TOX), which has rather pleiotropic functions during development and in cancer. In light of the most recent findings, this review gives an overview on the many different roles played by TOX family of proteins in physiological processes, and of the context-dependent effects that TOX induces in a variety of tumors. A particular attention is given to the contribution of TOX to the mechanism of T cell-exhaustion and to the "exhaustion lock" - no longer viewed as a dysfunctional immunological program but rather as a sophisticated strategy evolved by the immune system to keep control during events of chronic stress. The therapeutic potentials of TOX as valuable biomarker and target in cancer and immunotherapy as well as in adoptive cell therapies are also discussed. CI - Copyright (c) 2026 Zuccolotto, Borghese, Rebuli, Rosato and Tosi. FAU - Zuccolotto, Gaia AU - Zuccolotto G AD - Immunology and Molecular Oncology Diagnostics, Veneto Institute of Oncology IOV-IRCCS, Padova, Italy. FAU - Borghese, Lodovica AU - Borghese L AD - Immunology and Molecular Oncology Diagnostics, Veneto Institute of Oncology IOV-IRCCS, Padova, Italy. FAU - Rebuli, Alessia AU - Rebuli A AD - Department of Surgery, Oncology and Gastroenterology, University of Padua, Padova, Italy. FAU - Rosato, Antonio AU - Rosato A AD - Immunology and Molecular Oncology Diagnostics, Veneto Institute of Oncology IOV-IRCCS, Padova, Italy. AD - Department of Surgery, Oncology and Gastroenterology, University of Padua, Padova, Italy. FAU - Tosi, Anna AU - Tosi A AD - Immunology and Molecular Oncology Diagnostics, Veneto Institute of Oncology IOV-IRCCS, Padova, Italy. AD - Department of Surgery, Oncology and Gastroenterology, University of Padua, Padova, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 0 (TOX protein, human) RN - 0 (High Mobility Group Proteins) SB - IM MH - Humans MH - T-Cell Exhaustion MH - *Tumor Microenvironment/immunology/genetics MH - *Epigenesis, Genetic MH - Animals MH - *Neoplasms/immunology/genetics/therapy/pathology/metabolism MH - *High Mobility Group Proteins/metabolism/genetics/immunology MH - *Chromatin Assembly and Disassembly PMC - PMC13710973 OTO - NOTNLM OT - T cell exhaustion OT - TOX OT - cancer OT - epigenetics (chromatin remodeling) OT - tumor microenvironment COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:21 PHST- 2026/08/03 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:21 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fimmu.2026.1957165 [doi] PST - epublish SO - Front Immunol. 2026 Oct 13;17:1957165. doi: 10.3389/fimmu.2026.1957165. eCollection 2026. PMID- 42948248 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2297-1769 (Print) IS - 2297-1769 (Electronic) IS - 2297-1769 (Linking) VI - 13 DP - 2026 TI - Application of a convolutional neural network for automatic cytological diagnosis of 7 common canine tumors on whole slide images: a proof-of-concept. PG - 1922780 LID - 10.3389/fvets.2026.1922780 [doi] LID - 1922780 AB - BACKGROUND: Artificial intelligence may help improve speed and consistency of diagnostic veterinary cytology, but validated tools remain limited. OBJECTIVES: To develop and evaluate a convolutional neural network (CNN) for automatic classification of 7 common canine tumors on cytology whole-slide images (WSIs). METHODS: Ninety-one May-Grunwald Giemsa-stained canine cytology cases were selected, representing carcinoma, histiocytoma, lymphoma, mast cell tumor, melanoma, plasma cell tumor, and soft tissue sarcoma, together with a background class. Slides were tiled into 22,577 image patches (512 x 512 pixels) and manually labeled and split using a case-wise 3-fold design to avoid data leakage. An ImageNet-pretrained Inception-v3 CNN was fine-tuned for 8-class classification. Performance was assessed at tile level, Grad-CAM was used for qualitative interpretability analysis, and majority-vote prediction was further evaluated at ROI level using attention-guided inference and at WSI level using attention-free whole-slide inference. RESULTS: Mean test accuracy at tile level was 86.3%, with mean macro balanced accuracy of 92.0% and mean macro F1-score of 85.6%. Grad-CAM showed attention focused predominantly on cytologically relevant cellular regions. At ROI level, attention-guided inference achieved 100% Top1 accuracy (28/28 cases). At WSI level, attention-free whole-slide inference achieved 92.9% Top1 accuracy (26/28 cases), and the correct diagnosis was included within the top 3 predictions in all cases. CONCLUSION: Inception-v3 achieved high diagnostic performance on canine cytology WSIs. The results support the feasibility of both AI-assisted ROI-based decision support and fully automated whole-slide inference in veterinary cytology. These findings are proof-of-concept evidence from a curated, single-institution cohort of typical, well-differentiated cases and require validation on diagnostically challenging and external cases before clinical use. CI - Copyright (c) 2026 Attini, Newing, Oikonomidis, Tsouloufi and Ressel. FAU - Attini, M AU - Attini M AD - Independent Practitioner, Leggiuno, Italy. FAU - Newing, E AU - Newing E AD - Department of Veterinary Anatomy, Physiology and Pathology, Institute of Infection, Veterinary and Ecological Sciences, Faculty of Health & Life Sciences, University of Liverpool, Liverpool, United Kingdom. FAU - Oikonomidis, I L AU - Oikonomidis IL AD - Department of Veterinary Anatomy, Physiology and Pathology, Institute of Infection, Veterinary and Ecological Sciences, Faculty of Health & Life Sciences, University of Liverpool, Liverpool, United Kingdom. FAU - Tsouloufi, T K AU - Tsouloufi TK AD - Department of Veterinary Anatomy, Physiology and Pathology, Institute of Infection, Veterinary and Ecological Sciences, Faculty of Health & Life Sciences, University of Liverpool, Liverpool, United Kingdom. FAU - Ressel, L AU - Ressel L AD - Department of Veterinary Anatomy, Physiology and Pathology, Institute of Infection, Veterinary and Ecological Sciences, Faculty of Health & Life Sciences, University of Liverpool, Liverpool, United Kingdom. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Vet Sci JT - Frontiers in veterinary science JID - 101666658 PMC - PMC13711538 OTO - NOTNLM OT - canine OT - convolutional neural network OT - cytology OT - oncology OT - whole slide image COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:21 PHST- 2026/06/29 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:21 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fvets.2026.1922780 [doi] PST - epublish SO - Front Vet Sci. 2026 Oct 13;13:1922780. doi: 10.3389/fvets.2026.1922780. eCollection 2026. PMID- 42948242 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-2295 (Electronic) IS - 1664-2295 (Linking) VI - 17 DP - 2026 TI - Targeted muscle reinnervation after traumatic or elective upper-extremity amputation: a systematic review and meta-analysis. PG - 1946094 LID - 10.3389/fneur.2026.1946094 [doi] LID - 1946094 AB - OBJECTIVE: To systematically evaluate functional recovery and prosthetic control following targeted muscle reinnervation (TMR) or TMR-based reconstruction in patients with upper extremity amputation or severe brachial plexus injury. METHODS: PubMed, Embase, Web of Science, Scopus, and the Cochrane Library were searched for studies reporting standardized functional outcomes before and after TMR. Two reviewers independently performed study selection, data extraction, and methodological quality assessment using the Methodological Index for Non-Randomized Studies (MINORS). Because most studies did not report the standard deviation of paired changes or within-participant correlations, preoperative and postoperative summary data were synthesized using mean differences (MDs) or standardized mean differences (SMDs) as exploratory estimates. Potentially overlapping cohorts were identified by comparing study centers, recruitment periods, authorship, and participant characteristics. RESULTS: Six eligible reports representing five independent patient cohorts were included. MINORS scores ranged from 9 to 12 out of 16, indicating important methodological limitations. Postoperative Southampton Hand Assessment Procedure (SHAP) scores improved significantly (MD = 31.45, 95% CI 6.69-56.21), although substantial heterogeneity was observed (I (2) = 83%). Disabilities of the Arm, Shoulder and Hand (DASH) scores showed a significant reduction in self-reported disability after direction harmonization (MD = 21.91, 95% CI 10.85-32.97; I (2) = 0%). A single study reported a significant improvement in Action Research Arm Test (ARAT) scores (MD = 22.00, 95% CI 10.29-33.71), while another reported an increase in OPUS upper-extremity function scores (mean change = 2.7, 95% CI 2.3-3.2). Sensitivity analysis including combined TMR and osseointegration produced a similar DASH effect. CONCLUSION: Current evidence suggests that TMR-based reconstruction may improve prosthetic task performance and perceived upper-limb function. However, the findings remain preliminary because of small samples, heterogeneous procedures, incomplete paired-data reporting, and the frequent use of TMR within multicomponent reconstructive programmes. Larger prospective multicenter studies using standardized functional outcomes are required. SYSTEMATIC REVIEW REGISTRATION: https://www.crd.york.ac.uk/PROSPERO/view/CRD420251002627, identifier (CRD420251002627). CI - Copyright (c) 2026 Meng, Li, Xue, Wu, Ma, Zheng, Zheng and Xu. FAU - Meng, Fan-Long AU - Meng FL AD - Department of Orthopedics and Traumatology, Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China. FAU - Li, Ji-Ling AU - Li JL AD - Department of Rehabilitation Medicine, Shanghai University of Traditional Chinese Medicine Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, China. FAU - Xue, Xin AU - Xue X AD - Department of Rehabilitation Medicine, Shanghai University of Traditional Chinese Medicine Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, China. FAU - Wu, Jia-Jia AU - Wu JJ AD - Department of Rehabilitation Medicine, Shanghai University of Traditional Chinese Medicine Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, China. FAU - Ma, Jie AU - Ma J AD - Department of Rehabilitation Medicine, Shanghai University of Traditional Chinese Medicine Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, China. FAU - Zheng, Yu-Xin AU - Zheng YX AD - Department of Orthopedics and Traumatology, Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China. FAU - Zheng, Mou-Xiong AU - Zheng MX AD - Department of Orthopedics and Traumatology, Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China. FAU - Xu, Jian-Guang AU - Xu JG AD - Engineering Research Center for TCM Intelligent Rehabilitation, Ministry of Education, Shanghai University of Traditional Chinese Medicine, Shanghai, China. LA - eng PT - Journal Article PT - Meta-Analysis PT - Systematic Review DEP - 20261013 PL - Switzerland TA - Front Neurol JT - Frontiers in neurology JID - 101546899 SB - IM MH - Humans MH - *Muscle, Skeletal/innervation MH - *Upper Extremity/surgery/innervation MH - *Amputation, Surgical MH - Recovery of Function/physiology MH - *Nerve Regeneration/physiology MH - *Brachial Plexus/injuries/surgery MH - *Amputation, Traumatic/surgery PMC - PMC13711498 OTO - NOTNLM OT - brachial plexus injury OT - functional outcomes OT - myoelectric prosthesis OT - targeted muscle reinnervation OT - upper extremity amputation COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:21 PHST- 2026/07/23 00:00 [received] PHST- 2026/09/07 00:00 [revised] PHST- 2026/09/14 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:21 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fneur.2026.1946094 [doi] PST - epublish SO - Front Neurol. 2026 Oct 13;17:1946094. doi: 10.3389/fneur.2026.1946094. eCollection 2026. PMID- 42948240 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2753-4294 (Electronic) IS - 2753-4294 (Linking) VI - 4 IP - 4 DP - 2026 TI - A probabilistic framework reveals life course heterogeneity in global diet and healthy longevity associations. PG - e005263 LID - 10.1136/bmjph-2026-005263 [doi] LID - e005263 AB - INTRODUCTION: Conventional dietary adherence scores may limit cross-population comparability and allow strong components to offset marked shortfalls. We developed a probabilistic framework to place established dietary patterns on a common scale while retaining information on limiting components. METHODS: We constructed the Dietary Matching Index (DMI) for eight dietary patterns using age-specific and sex-specific dietary estimates from 185 countries from 1990 to 2018. Parameters were selected using outcome-blind sensitivity analyses. Construct validity was assessed using global dietary data and six harmonised individual-level dietary datasets. Spatial clustering was evaluated using global and local Moran's I. Associations with health-adjusted life expectancy (HALE) were estimated using geographically and temporally weighted regression models adjusted for sex and socioeconomic and ecological covariates. RESULTS: DMI rankings were robust to calibration choices and Monte Carlo resampling. Across joint parameter settings, minimum Spearman correlations with baseline rankings ranged from 0.971 to 0.993, and Spearman correlations between the 250-draw and 500-draw estimates were 1.00 for all eight patterns. Component-level analyses reproduced the intended structure of the original dietary patterns, and individual-level gradients were consistent across six validation datasets. Dietary alignment varied substantially across countries and showed persistent geographic clustering. DMI-based models showed comparable or modestly better fit than conventional-score models. Associations with HALE were modest and varied by age and region. The largest positive pooled association was 0.17 additional HALE years for a one-standard-deviation higher Mediterranean-style DMI in ages 2-19 years (95% uncertainty interval 0.08 to 0.22). Between-country heterogeneity was greatest in midlife, with I(2) exceeding 50% for most patterns. CONCLUSIONS: DMI provides a common probabilistic scale for comparing dietary patterns and identifying limiting components. Dietary alignment showed persistent geographic structure, while associations with HALE varied by age and region. These ecological findings may support dietary surveillance and prioritisation, but causal interpretation requires evidence from longitudinal or intervention studies. CI - (c) Author(s) (or their employer(s)) 2026. Re-use permitted under CC BY-NC. Published by BMJ Group. FAU - Pan, Yuzhe AU - Pan Y AD - Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - School of Nursing, Wuhan University, Wuhan, China. FAU - Zhang, Nan AU - Zhang N AD - Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - Department of Hematology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China. FAU - Tong, Xiqin AU - Tong X AD - Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Chen, Guopeng AU - Chen G AD - Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Li, Xinqi AU - Li X AD - Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Wu, Jinxian AU - Wu J AD - Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Fang, Jiamin AU - Fang J AD - Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. FAU - Li, Duantengchuan AU - Li D AD - School of Information Management, Wuhan University, Wuhan, China. FAU - Wu, Liutao AU - Wu L AD - School of Nursing, Wuhan University, Wuhan, China. FAU - Peng, Shuang AU - Peng S AD - School of Nursing, Wuhan University, Wuhan, China. FAU - Liu, Qian AU - Liu Q AD - School of Nursing, Wuhan University, Wuhan, China. FAU - Zhou, Fuling AU - Zhou F AUID- ORCID: 0000-0003-0982-0382 AD - Department of Hematology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, China. AD - School of Nursing, Wuhan University, Wuhan, China. AD - Research Center for Lifespan Health, Wuhan University, Wuhan, Hubei, China. LA - eng PT - Journal Article DEP - 20261016 PL - England TA - BMJ Public Health JT - BMJ public health JID - 9918697578906676 PMC - PMC13711543 OTO - NOTNLM OT - Nutrition Assessment OT - Public Health OT - Space-Time Clustering COIS- Competing interests: The authors report institutional research support from the Smart Health Research and Development Center jointly established by Wuhan University and Hubei Yijiantang Smart Technology Group Co., Ltd. in support of this work. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/16 CRDT- 2026/10/28 05:21 PHST- 2026/02/25 00:00 [received] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:21 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - bmjph-2026-005263 [pii] AID - 10.1136/bmjph-2026-005263 [doi] PST - epublish SO - BMJ Public Health. 2026 Oct 16;4(4):e005263. doi: 10.1136/bmjph-2026-005263. eCollection 2026. PMID- 42948233 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2008-2010 (Print) IS - 2008-322X (Electronic) IS - 2008-322X (Linking) VI - 21 DP - 2026 TI - Molecular Identification of Biofilm-producing Staphylococcus aureus in Contact Lens Storage Solutions. LID - 10.18502/jovr.v21.13795 [pii] AB - PURPOSE: This study aimed to evaluate the presence of biofilm-producing Staphylococcus aureus in contact lens storage solutions (CLSS) used for both cosmetic and vision-correcting purposes. Microbiological assays and molecular methods were used to detect biofilm-associated genes, specifically icaA and icaD. The findings could provide molecular evidence of contamination in CLSS and identify behavioral risk factors associated with biofilm-forming strains. METHODS: A total of 162 CLSS samples were collected from asymptomatic students living in dormitories and hospital staff. Bacterial isolation and identification were performed using standard culture methods and biochemical tests. Biofilm formation was evaluated phenotypically, and molecular confirmation of S. aureus and biofilm-associated genes (icaA and icaD) was performed using polymerase chain reaction (PCR). Associations between contamination and lens type, and also user behaviors, including lens-washing practices, showering, and swimming while wearing lenses, were analyzed. RESULTS: This study evaluated 162 CLSS samples from 162 asymptomatic participants (mean age: 24.8 +/- 4.2 years). Of these, 148 subjects (91.35%) were females. Staphylococcus aureus contamination was detected in7 of 162 samples (4.32%),and 4 of the 7 isolates (57.14%) carried biofilm-associated icaA and icaD genes. Staphylococcus aureus contamination was found in 5 of 108 CLSS used for vision-correcting purposes (4.62%; P = 0.53). Contamination was significantly associated with lack of lens washing before wearing, which occurred in 4 of 141 participants (2.83%; P = 0.005), and lack of hand washing before handling contact lenses, which was observed in 7 of 159 participants (4.40%; P = 0.043). CONCLUSION: This study provides molecular evidence that CLSS can act as reservoirs for biofilm-producing S. aureus. The detection of icaA- and icaD-positive strains highlights the risk of persistent contamination and the potential development of contact lens-related infectious keratitis. Modifiable behaviors, especially proper lens washing, may significantly reduce the risk of contamination with biofilm-producing S. aureus. CI - Copyright (c) 2026 Atighehchian et al. FAU - Atighehchian, Mehrnaz AU - Atighehchian M AD - Jahrom University of Medical Sciences, Jahrom, Iran. AD - Noor Ophthalmology Research Center, Noor Eye Hospital, Tehran, Iran. FAU - Shabani, Manoochehr AU - Shabani M AD - Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran. FAU - Abedinifar, Zohreh AU - Abedinifar Z AD - Microbiology Unit, Department of Pathology, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran. FAU - Manoochehri, Zahra AU - Manoochehri Z AD - Jahrom University of Medical Sciences, Jahrom, Iran. FAU - Zarei-Ghanavati, Mehran AU - Zarei-Ghanavati M AD - Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran. LA - eng PT - Journal Article DEP - 20260923 PL - United Arab Emirates TA - J Ophthalmic Vis Res JT - Journal of ophthalmic & vision research JID - 101497643 PMC - PMC13710837 OTO - NOTNLM OT - Keratitis OT - Staphylococcus aureus OT - ica Genes OT - Biofilm OT - Contact Lens Storage Solution COIS- None. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/09/23 CRDT- 2026/10/28 05:20 PHST- 2023/07/28 00:00 [received] PHST- 2025/12/08 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:20 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - 10.18502/jovr.v21.13795 [pii] AID - 10.18502/jovr.v21.13795 [doi] PST - epublish SO - J Ophthalmic Vis Res. 2026 Sep 23;21:10.18502/jovr.v21.13795. doi: 10.18502/jovr.v21.13795. eCollection 2026. PMID- 42948228 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 0860-021X (Print) IS - 2083-1862 (Electronic) IS - 0860-021X (Linking) VI - 43 DP - 2026 Jan TI - Analysis of key performance indicators influencing offensive effectiveness during clutch moments in elite international men's basketball. PG - 1663-1675 LID - 10.5114/biolsport.2026.163367 [doi] AB - Clutch moments often determine the final outcome of basketball games. The present study proposes an innovative analytical framework that integrates machine learning with explainable techniques to identify and quantify the influence of technical-tactical indicators on possession-level scoring outcomes during clutch moments. Using an observational methodology, 607 clutch offensive possessions from 41 games of the 2019 and 2023 FIBA Men's Basketball World Cups were coded and analyzed. First, chi-square tests were applied to examine associations between technical-tactical variables and offensive outcomes (scoring vs. non-scoring). Subsequently, ten categories of machine learning models were systematically compared, with the Random Forest identified as the best-performing model for predicting scoring outcomes in clutch possessions. Finally, SHAP values were used to interpret the contribution and directional effects of each variable on model outputs. The results indicated that play-types (p < 0.001), possession duration (p = 0.009), and ending zone (p = 0.001) were significantly associated with scoring success. SHAP analysis further showed that "restricted area" "possessions completed within 1-8 seconds," and "ending player position_center" were positively associated with scoring success. These findings provide data-driven support and practical reference for coaches and performance analysts when designing training priorities and tactical strategies in clutch contexts. Specifically, during clutch moments of elite international competition, offenses may benefit from selectively increasing tempo to break deadlocks and emphasizing team-oriented actions by increasing center involvement as primary finishers and strengthening screening and pick-and-roll actions. CI - Copyright (c) Institute of Sport - National Research Institute. FAU - Chen, Zhengyang AU - Chen Z AD - School of Athletic Performance, Shanghai University of Sport, Shanghai, China. AD - Shanghai Key Lab of Human Performance (Shanghai University of Sport), Shanghai, China. FAU - Qiu, Mingjia AU - Qiu M AD - School of Athletic Performance, Shanghai University of Sport, Shanghai, China. AD - Shanghai Key Lab of Human Performance (Shanghai University of Sport), Shanghai, China. FAU - Sansone, Pierpaolo AU - Sansone P AD - Department of Education and Sport Sciences, Pegaso Telematic University, Naples, Italy. FAU - Gomez, Miguel-Angel AU - Gomez MA AD - Faculty of Physical Activity and Sport Sciences. Universidad Politecnica de Madrid, Madrid, Spain. FAU - Zhang, Mingxin AU - Zhang M AD - School of Athletic Performance, Shanghai University of Sport, Shanghai, China. AD - Shanghai Key Lab of Human Performance (Shanghai University of Sport), Shanghai, China. LA - eng PT - Journal Article DEP - 20260727 PL - Poland TA - Biol Sport JT - Biology of sport JID - 8700872 PMC - PMC13711642 OTO - NOTNLM OT - Clutch time OT - FIBA Basketball World Cup OT - Machine learning OT - Notational analysis OT - Performance analysis COIS- The authors declare no conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/07/27 CRDT- 2026/10/28 05:20 PHST- 2025/11/27 00:00 [received] PHST- 2026/01/28 00:00 [revised] PHST- 2026/07/07 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:20 [entrez] PHST- 2026/07/27 00:00 [pmc-release] AID - 58394 [pii] AID - 10.5114/biolsport.2026.163367 [doi] PST - epublish SO - Biol Sport. 2026 Jul 27;43:1663-1675. doi: 10.5114/biolsport.2026.163367. eCollection 2026 Jan. PMID- 42948225 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-2565 (Electronic) IS - 2296-2565 (Linking) VI - 14 DP - 2026 TI - Artificial intelligence for detecting autism spectrum disorder: a systematic review and meta-analysis. PG - 1923851 LID - 10.3389/fpubh.2026.1923851 [doi] LID - 1923851 AB - BACKGROUND: Autism spectrum disorder (ASD) is a heterogeneous neurodevelopmental condition, and early identification is essential for timely intervention. Conventional diagnosis relies on clinical interviews, behavioral observations, caregiver reports, and standardized tools, which are time-consuming and resource-dependent. Artificial intelligence (AI) offers opportunities for screening and adjunctive diagnosis, but studies vary in data sources, model types, and validation methods, requiring systematic evaluation. METHODS: We searched PubMed, Embase, the Cochrane Library, Web of Science, and IEEE Xplore for studies of AI-based ASD detection. Eligible studies were required to provide ASD-versus-non-ASD diagnostic-accuracy data that could be extracted or reliably reconstructed at the independent participant or case level. Risk of bias and applicability were assessed using a modified QUADAS-AI tool. Pooled sensitivity and specificity were estimated with a bivariate Reitsma random-effects model. HSROC AUCs were calculated with bias-corrected and accelerated (BCa) 95% confidence intervals based on 5,000 study-level bootstrap replicates and were omitted when SROC fits were unstable. Sensitivity analyses addressed dataset overlap, model selection, and reference-standard quality. RESULTS: Of 17,387 records, 26 studies were included. Pooled sensitivity was 0.908 (95% CI: 0.861-0.940), pooled specificity was 0.868 (95% CI: 0.830-0.898), and the HSROC AUC was 0.936 (BCa 95% CI: 0.860-0.973). A dataset-independent analysis of 21 studies yielded lower estimates (sensitivity 0.870, specificity 0.841, AUC 0.900). Leave-one-out results were generally stable. Funnel-plot asymmetry was not significant in the primary analysis and varied in direction and statistical significance across sensitivity analyses. CONCLUSIONS: AI-based models showed relatively high pooled diagnostic performance for ASD detection, but substantial heterogeneity, methodological and applicability concerns, limited external validation, and potential non-independence of underlying datasets limit the certainty and generalizability of the evidence. Funnel-plot analyses did not provide consistent evidence of conventional small-study effects or selective reporting. AI should therefore be considered an adjunct rather than a standalone diagnostic tool. Prospective multicenter validation in representative populations, participant-level evaluation, clearly defined reference diagnoses, prespecified models and operating thresholds, and independent evaluation datasets are needed before broader clinical implementation. CI - Copyright (c) 2026 Wang, Luo and Wen. FAU - Wang, Qian AU - Wang Q AD - Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China. AD - Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, China. FAU - Luo, Rong AU - Luo R AD - Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China. AD - Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, China. FAU - Wen, Qianhui AU - Wen Q AD - Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China. AD - Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, China. LA - eng PT - Journal Article PT - Meta-Analysis PT - Systematic Review DEP - 20261012 PL - Switzerland TA - Front Public Health JT - Frontiers in public health JID - 101616579 SB - IM MH - *Autism Spectrum Disorder/diagnosis MH - *Artificial Intelligence MH - Humans MH - Sensitivity and Specificity PMC - PMC13710828 OTO - NOTNLM OT - artificial intelligence OT - autism spectrum disorder OT - diagnosis OT - machine learning OT - meta-analysis COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/12 CRDT- 2026/10/28 05:20 PHST- 2026/06/30 00:00 [received] PHST- 2026/08/28 00:00 [revised] PHST- 2026/08/31 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:20 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - 10.3389/fpubh.2026.1923851 [doi] PST - epublish SO - Front Public Health. 2026 Oct 12;14:1923851. doi: 10.3389/fpubh.2026.1923851. eCollection 2026. PMID- 42948217 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-302X (Print) IS - 1664-302X (Electronic) IS - 1664-302X (Linking) VI - 17 DP - 2026 TI - Advances in diabetic foot ulcer management: a narrative review of novel antibiotics, nanotechnology, and regenerative therapeutics. PG - 1882663 LID - 10.3389/fmicb.2026.1882663 [doi] LID - 1882663 AB - Diabetic foot ulcers (DFUs) are a major cause of hospitalization, chronic infection, and non-traumatic lower-limb amputation, imposing substantial clinical and economic burdens worldwide. Standard DFU management requires a multidisciplinary approach involving pressure offloading, regular debridement, infection control, metabolic optimisation, vascular assessment, and revascularisation when indicated. Offloading and sharp debridement reduce mechanical stress, remove devitalized tissue, disrupt biofilms, and support wound healing. Infected ulcers require appropriate microbiological assessment and culture-directed antimicrobial therapy, particularly when multidrug-resistant (MDR) pathogens are present. Biofilm formation is an important feature of chronic DFUs and is associated with persistent infection, delayed healing, and treatment failure. Biofilm-associated antimicrobial tolerance results from factors including reduced antimicrobial penetration, metabolic dormancy, altered growth states, and phenotypic heterogeneity, and should be distinguished from genetically mediated antimicrobial resistance caused by acquired or chromosomal genetic determinants. Although biofilm-associated tolerance does not necessarily involve genetic resistance, persistent biofilm infection may facilitate the selection and persistence of resistant subpopulations. Both Gram-positive and Gram-negative organisms can form biofilms in DFUs. Novel antibiotics, including oxazolidinones, lipoglycopeptides, lipopeptides, and advanced beta-lactam/beta-lactamase inhibitor combinations, may provide options for selected difficult to treat infections, although evidence varies among agents and clinical settings. Adjunctive wound-care strategies, including appropriate dressings and negative-pressure wound therapy, may support wound-bed preparation and healing in selected cases. Emerging approaches such as nanoparticle-based delivery systems, hydrogels, nanofibrous scaffolds, stem-cell and exosome therapies, gene and RNA-based strategies, and oxygen-releasing biomaterials have shown potential in preclinical and early clinical studies. However, their clinical effectiveness, safety, reproducibility, scalability, and regulatory approval and clinical translation remain incompletely established. Integrating evidence-based standard care with appropriate antimicrobial therapy and carefully evaluated emerging technologies may facilitate individualized management of complex DFUs. CI - Copyright (c) 2026 Saleem, Habib Khan, Almarshedy, Alenazi, Alshetaiwi, Ahmad, Alrashid, Ilyas, Almuwaisa, Alnomasi and Khan. FAU - Saleem, Mohd AU - Saleem M AD - Department of Pathology, College of Medicine, University of Hail, Hail, Saudi Arabia. FAU - Habib Khan, Farida AU - Habib Khan F AD - Department of Family and Community Medicine, College of Medicine, University of Hail, Hail, Saudi Arabia. FAU - Almarshedy, Sumayyah Mohammad AU - Almarshedy SM AD - Department of Medicine, College of Medicine, University of Hail, Hail, Saudi Arabia. FAU - Alenazi, Fahaad S AU - Alenazi FS AD - Department of Pharmacology, Medical Education Committee, College of Medicine, University of Hail, Hail, Saudi Arabia. FAU - Alshetaiwi, Hamad AU - Alshetaiwi H AD - Department of Pathology, College of Medicine, University of Hail, Hail, Saudi Arabia. FAU - Ahmad, Irfan AU - Ahmad I AD - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia. AD - Health and Medical Research Center, King Khalid University, Abha, Saudi Arabia. FAU - Alrashid, Fauwaz Fahad AU - Alrashid FF AD - Department of Surgery, College of Medicine, University of Hail, Hail, Saudi Arabia. FAU - Ilyas, Syed Muhammad Ozair AU - Ilyas SMO AD - Department of General Surgery, King Khalid Hospital, Hail, Saudi Arabia. FAU - Almuwaisa, Abdulrahman Moussa AU - Almuwaisa AM AD - Department of Family and Community Medicine, College of Medicine, University of Hail, Hail, Saudi Arabia. FAU - Alnomasi, Wael Nahi AU - Alnomasi WN AD - Department of Family and Community Medicine, College of Medicine, University of Hail, Hail, Saudi Arabia. FAU - Khan, Mohd Shahid AU - Khan MS AD - Department of Microbiology, Hind Institute of Medical Sciences, Sitapur, Uttar Pradesh, India. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Microbiol JT - Frontiers in microbiology JID - 101548977 PMC - PMC13710819 OTO - NOTNLM OT - biofilm OT - diabetic foot ulcer OT - multidrug-resistant infections OT - nanotechnology-based drug delivery OT - wound healing therapies COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:19 PHST- 2026/05/15 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:19 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fmicb.2026.1882663 [doi] PST - epublish SO - Front Microbiol. 2026 Oct 13;17:1882663. doi: 10.3389/fmicb.2026.1882663. eCollection 2026. PMID- 42948211 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2673-253X (Electronic) IS - 2673-253X (Linking) VI - 8 DP - 2026 TI - Research on the construction of a digital medical consumables asset management system based on information ecology theory. PG - 1946134 LID - 10.3389/fdgth.2026.1946134 [doi] LID - 1946134 AB - Medical consumables constitute a core object of hospital asset management, and the traditional extensive management model can no longer meet the requirements of refined management in modern hospitals. This narrative review aims to construct a conceptual theoretical framework based on information ecology theory, to analyze the patterns of information flow in the field of medical consumables management, and build a digital asset management ecosystem centered on the four elements of "information platform-digital technology-responsible actor-social environment". As a narrative review, it synthesizes selected literature and policy documents rather than providing a systematic or exhaustive synthesis of evidence. The conceptual contribution of this framework is to address the "technology-heavy, system-light" limitation of existing research by shifting toward ecological governance through the coordination of the four elements, and proposing an operating mechanism in which the information chain links the closed loop of asset management. The framework is illustrated through a worked example of high-value consumable use during cesarean section. The framework is illustrated through a worked example of high-value consumable use during cesarean section. It provides a conceptual reference and a hypothesis-generating roadmap for future research and local adaptation, rather than a validated operational solution. All thresholds, targets, automated responses, and organizational arrangements mentioned in this article are proposed design principles for future validation, not empirically confirmed standards. CI - (c) 2026 Wan, Yang, Cai, Wang and He. FAU - Wan, Qi AU - Wan Q AD - Asset Management Department, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai, China. FAU - Yang, Yifan AU - Yang Y AD - Asset Management Department, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai, China. FAU - Cai, Meihua AU - Cai M AD - Asset Management Department, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai, China. FAU - Wang, Ke AU - Wang K AD - Asset Management Department, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai, China. FAU - He, Kai AU - He K AD - Asset Management Department, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai, China. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Digit Health JT - Frontiers in digital health JID - 101771889 PMC - PMC13711402 OTO - NOTNLM OT - asset management OT - digital technology OT - information ecology theory OT - life-cycle management OT - medical consumables COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:19 PHST- 2026/07/23 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:19 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fdgth.2026.1946134 [doi] PST - epublish SO - Front Digit Health. 2026 Oct 13;8:1946134. doi: 10.3389/fdgth.2026.1946134. eCollection 2026. PMID- 42948200 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2297-1769 (Print) IS - 2297-1769 (Electronic) IS - 2297-1769 (Linking) VI - 13 DP - 2026 TI - Prognostic significance of EGFR-1 and VEGF-A expression in feline cutaneous squamous cell carcinoma. PG - 1949867 LID - 10.3389/fvets.2026.1949867 [doi] LID - 1949867 AB - INTRODUCTION: Cutaneous squamous cell carcinoma (cSCC) is a prevalent malignancy in cats, and advanced cases remain challenging to manage due to their high potential for local invasion and recurrence. Epidermal growth factor receptor 1 (EGFR-1) and vascular endothelial growth factor A (VEGF-A) have emerged as promis- ing prognostic biomarkers in epithelial tumors, given their roles in proliferation, invasion, and angiogenesis. This study aimed to evaluate EGFR-1 and VEGF-A immunoexpression in cats with cSCC treated with electrochemotherapy (ECT) and their associations with clinicopathological variables and survival outcomes. METHODS: Immunohistochemical expression of EGFR-1 and VEGF-A was evaluated in tumor samples from 33 cats with cSCC enrolled prospectively (n = 24) and retrospec- tively (n = 9). Associations between immunoexpression scores and clinicopatho- logical variables, overall survival time (OST), disease-free interval (DFI), and time to progression (TTP) were assessed using appropriate statistical tests. RESULTS: EGFR-1 positivity was detected in 78.8% (26/33) of cases and was significantly associated with shorter OST, perineural invasion, and intense inflammatory infiltrate. VEGF-A immunoexpression was detected in 71.9% (23/32) of cases and was significantly associated with previous history of cSCC, perineural invasion (PNI), and tumor invasion beyond the subcutaneous fat. DISCUSSION: These findings suggest that EGFR-1 and VEGF-A immunoexpression are associated with clinicopathological variables linked to aggressive tumor behavior in feline cSCC, supporting their potential role as prognostic biomarkers in this species and warranting further investigation in larger cohorts. CI - Copyright (c) 2026 Linhares, Rangel, de Oliveira, Fonseca-Alves, de Moura, Bispo, Queiroga and de Nardi. FAU - Linhares, Lais Calazans Menescal AU - Linhares LCM AD - Department of Veterinary Clinic and Surgery, School of Agricultural and Veterinary Sciences, Sao Paulo State University, Jaboticabal, Sao Paulo, Brazil. FAU - Rangel, Marcelo Monte Mor AU - Rangel MMM AD - Vet Cancer Veterinary Oncology, Brooklin, Sao Paulo, Brazil. FAU - de Oliveira, Krishna Duro AU - de Oliveira KD AD - VCLab Pathology, Pinheiros, Sao Paulo, Brazil. FAU - Fonseca-Alves, Carlos Eduardo AU - Fonseca-Alves CE AD - Department of Veterinary Surgery and Animal Reproduction, Sao Paulo State University-UNESP, Botucatu, Sao Paulo, Brazil. FAU - de Moura, Fernanda Barthelson Carvalho AU - de Moura FBC AD - Department of Veterinary Surgery and Animal Reproduction, Sao Paulo State University-UNESP, Botucatu, Sao Paulo, Brazil. FAU - Bispo, Guilherme Andraus AU - Bispo GA AD - School of Veterinary Medicine, Sao Paulo State University-UNESP, Aracatuba, Sao Paulo, Brazil. FAU - Queiroga, Felisbina L AU - Queiroga FL AD - Animal and Veterinary Science Research Center (CECAV), University of Tras-os-Montes and Alto Douro, Vila Real, Portugal. FAU - de Nardi, Andrigo Barboza AU - de Nardi AB AD - Department of Veterinary Clinic and Surgery, School of Agricultural and Veterinary Sciences, Sao Paulo State University, Jaboticabal, Sao Paulo, Brazil. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Vet Sci JT - Frontiers in veterinary science JID - 101666658 PMC - PMC13711497 OTO - NOTNLM OT - EGFR-1 OT - VEGF-A OT - cat OT - electrochemotherapy OT - immunohistochemistry OT - prognosis OT - skin cancer COIS- Author KO was employed by company VCLab Pathology. The remaining author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:18 PHST- 2026/07/27 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:18 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fvets.2026.1949867 [doi] PST - epublish SO - Front Vet Sci. 2026 Oct 13;13:1949867. doi: 10.3389/fvets.2026.1949867. eCollection 2026. PMID- 42948192 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2666-2736 (Electronic) IS - 2666-2736 (Linking) VI - 33 DP - 2026 Oct TI - Differences in patterns of relapse and survival after relapse between lobectomy and sublobar resection for stage IA non-small cell lung cancer. PG - 101952 LID - 10.1016/j.xjon.2026.101952 [doi] LID - 101952 AB - OBJECTIVE: In the Japan Clinical Oncology Group 0802 trial, lung cancer-specific mortality was comparable between lobectomy and segmentectomy despite a higher rate of locoregional relapse after segmentectomy. We evaluated relapse patterns and postrelapse survival following lobectomy or sublobar resection. METHODS: This single-institution retrospective study included patients who underwent surgery for primary non-small cell lung cancer (NSCLC) between 2003 and 2022 and subsequently developed relapse. Overall survival was defined as the period from surgery (OS1) or from the date of relapse diagnosis (OS2) to death from any cause. RESULTS: Among 362 patients with clinical stage IA NSCLC who developed relapse, isolated locoregional relapse accounted for 110 of 297 relapsed patients (37.0%) after lobectomy and 36 of 65 relapsed patients (55.4%) after sublobar resection. Among 59 patients treated with local consolidative therapy for isolated locoregional relapse, the 4-year OS2 rate was 76.4% (95% CI, 64.9%-89.8%). After inverse probability of treatment weighting, the 7-year OS1 rates were 49.3% (95% CI, 42.2%-57.6%) after lobectomy and 64.9% (95% CI, 52.4%-80.3%) after sublobar resection, and the 4-year OS2 rates were 49.6% (95% CI, 42.4%-57.9%) and 62.7% (95% CI, 49.5%-79.3%), respectively. CONCLUSIONS: In this exploratory cohort of patients who developed postoperative relapse, relapse patterns differed between surgical groups, with isolated locoregional relapse representing a larger proportion of relapse in the sublobar resection group. Long-term survival was observed in selected patients treated with modern postrelapse therapies, including local consolidative therapy. These findings should be interpreted cautiously as hypothesis-generating. CI - (c) 2026 The Author(s). FAU - Adachi, Masahiro AU - Adachi M AD - Department of Thoracic Surgery, National Cancer Center Hospital, Tokyo, Japan. FAU - Yoshida, Yukihiro AU - Yoshida Y AD - Department of Thoracic Surgery, National Cancer Center Hospital, Tokyo, Japan. FAU - Takemura, Chihiro AU - Takemura C AD - Department of Thoracic Surgery, National Cancer Center Hospital, Tokyo, Japan. FAU - Yotsukura, Masaya AU - Yotsukura M AD - Department of Thoracic Surgery, National Cancer Center Hospital, Tokyo, Japan. FAU - Haruki, Tomohiro AU - Haruki T AD - Department of Thoracic Surgery, National Cancer Center Hospital, Tokyo, Japan. FAU - Horinouchi, Hidehito AU - Horinouchi H AD - Department of Thoracic Oncology, National Cancer Center Hospital, Tokyo, Japan. FAU - Goto, Yasushi AU - Goto Y AD - Department of Thoracic Oncology, National Cancer Center Hospital, Tokyo, Japan. FAU - Watanabe, Shun-Ichi AU - Watanabe SI AD - Department of Thoracic Surgery, National Cancer Center Hospital, Tokyo, Japan. LA - eng PT - Journal Article DEP - 20260704 PL - Netherlands TA - JTCVS Open JT - JTCVS open JID - 101768541 PMC - PMC13711452 OTO - NOTNLM OT - local consolidative treatment OT - locoregional relapse OT - oncologic outcomes OT - optimal surgical procedure OT - postrelapse therapy OT - relapse pattern OT - sublobar resection COIS- Dr Yoshida reports research grants from AstraZeneca, Chugai Pharmaceutical, and Takahata Precision, and lecture fees from Medtronic and Kyorin Pharmaceutical, outside the submitted work. Dr Horinouchi reports research grants from Natera, AbbVie, AstraZeneca, Bristol-Myers Squibb (BMS), Ono Pharmaceutical, Daiichi-Sankyo, Janssen, and Chugai Pharmaceutical; lecture fees from AstraZeneca, MSD, Ono Pharmaceutical, BMS, Chugai Pharmaceutical, Amgen, and AbbVie; and honoraria for advisory boards from AstraZeneca, Chugai Pharmaceutical, Ono Pharmaceutical, BMS, and AbbVie, outside the submitted work. Dr Goto reports research grants to his institution from IQVIA Services Japan, MSD, Astellas Pharma, AstraZeneca, AbbVie, Amgen, Syneos Health Clinical, Sysmex Corporation, CMIC, Novartis Pharma, Bayer Pharmaceuticals, BMS, MedPace Japan, Janssen Pharma, Clinical Research Support Center Kyushu, SATOMI, Ono Pharmaceutical, Daiichi Sankyo, Takeda Pharmaceutical, Chugai Pharmaceutical, NPO Thoracic Oncology Research Group, Eli Lilly Japan, and Preferred Network. He also reports research grants to himself from AstraZeneca, AbbVie, Eli Lilly, Pfizer, BMS, Ono Pharmaceutical, Novartis, Kyorin Pharmaceutical, Daiichi-Sankyo, MSD, Guardant Health, and Preferred Network; lecture fees from Eli Lilly, Chugai Pharmaceutical, Taiho Pharmaceutical, Boehringer Ingelheim, Ono Pharmaceutical, BMS, Pfizer, MSD, Novartis, Merck, and Thermo Fisher Scientific; and honoraria for advisory boards from AstraZeneca, Chugai Pharmaceutical, Boehringer Ingelheim, Eli Lilly, GlaxoSmithKline, Taiho Pharmaceutical, Pfizer, Novartis, Kyorin Pharmaceutical, Guardant Health, Illumina, Daiichi-Sankyo, Merck, MSD, Ono Pharmaceutical, and Janssen. Additionally, he serves in leadership or fiduciary roles for Cancer Net Japan and JAMT, outside the submitted work. All other authors reported no conflicts of interest. The Journal policy requires editors and reviewers to disclose conflicts of interest and to decline handling or reviewing manuscripts for which they may have a conflict of interest. The editors and reviewers of this article have no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/07/04 CRDT- 2026/10/28 05:18 PHST- 2026/04/02 00:00 [received] PHST- 2026/06/03 00:00 [revised] PHST- 2026/06/28 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:18 [entrez] PHST- 2026/07/04 00:00 [pmc-release] AID - S2666-2736(26)00375-X [pii] AID - 101952 [pii] AID - 10.1016/j.xjon.2026.101952 [doi] PST - epublish SO - JTCVS Open. 2026 Jul 4;33:101952. doi: 10.1016/j.xjon.2026.101952. eCollection 2026 Oct. PMID- 42948170 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2813-2092 (Electronic) IS - 2813-2092 (Linking) VI - 5 DP - 2026 TI - Posterior rectus sheath reattachment after eTEP rives: are we solving the right problem? PG - 17986 LID - 10.3389/jaws.2026.17986 [doi] LID - 17986 FAU - Daes, Jorge AU - Daes J AD - Division of Minimally Invasive and Bariatric Surgery, Department of Surgery, Clinica Iberoamerica, Barranquilla, Colombia. LA - eng PT - Editorial DEP - 20261013 PL - Switzerland TA - J Abdom Wall Surg JT - Journal of abdominal wall surgery : JAWS JID - 9918713888406676 PMC - PMC13711373 OTO - NOTNLM OT - abdominal wall morphology OT - abdominal wall reconstruction OT - biomechanics OT - bulging OT - eTEP COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:17 PHST- 2026/10/04 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:17 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 17986 [pii] AID - 10.3389/jaws.2026.17986 [doi] PST - epublish SO - J Abdom Wall Surg. 2026 Oct 13;5:17986. doi: 10.3389/jaws.2026.17986. eCollection 2026. PMID- 42948166 OWN - NLM STAT- Publisher LR - 20261029 IS - 2046-2069 (Electronic) IS - 2046-2069 (Linking) DP - 2026 Oct 27 TI - A novel magnetic N-rich covalent triazine framework-copper(ii) complex as an efficient nanocatalyst for Ullmann coupling. LID - 10.1039/d6ra09208j [doi] AB - In the present work, we describe the development of a new nitrogen-rich polymer grafted onto hercynite magnetic nanoparticles (MNPs) for the stabilization of a copper catalyst. In this approach, the surface hydroxyl groups of hercynite were covalently bonded to cyanuric chloride, which was subsequently polymerized with melamine to generate a nitrogen-rich covalent triazine polymer network around the hercynite surface. This framework was then coordinated with copper to yield a new heterogeneous nanomagnetic copper catalyst. The as-prepared [hercynite@TCOP-Cu(ii)] was applied in Ullmann C-O coupling of various aryl halides with phenolic substrates using PEG-400 as an environmentally benign reaction medium, furnishing the corresponding diaryl ethers in good to excellent yields with high selectivity and short reaction times. Furthermore, the use of aniline and mercaptophenol provided the corresponding diarylamines and diaryl sulfides in excellent yields, confirming the generality and broad substrate scope of this methodology, as well as its efficiency in C-N and C-S cross-coupling reactions in addition to C-O couplings. The catalytic system exhibited a heterogeneous nature, excellent stability, and reusability over at least six consecutive recovery cycles via magnetic separation with minimal loss of activity. These findings highlight the potential of this nanocomposite as a practical, sustainable, and highly efficient catalyst for Ullmann-type coupling reactions. CI - This journal is (c) The Royal Society of Chemistry. FAU - Hasan, Mohammed Obaid AU - Hasan MO AD - Ministry of Higher Education and Scientific Research, Research and Development Directorate Baghdad Iraq. FAU - Ahmed, Athraa H AU - Ahmed AH AD - Ministry of Higher Education and Scientific Research, Directorate of Studies, Planning and Follow-up Baghdad Iraq. FAU - Hmood, Kasim S AU - Hmood KS AD - Department of Pharmaceutical Chemistry, College of Pharmacy, University of Kut Wasit 52001 Iraq. FAU - Faisal, Alaa Hamid AU - Faisal AH AUID- ORCID: 0009-0005-4910-2715 AD - College of Health and Medical Technology, Al-Ayen Iraqi University AUIQ Thi-Qar Iraq. FAU - Muneer, Ali AU - Muneer A AD - Department of Chemistry, College of Science, Mustansiriyah University Baghdad Iraq. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - RSC Adv JT - RSC advances JID - 101581657 PMC - PMC13711382 COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/27 CRDT- 2026/10/28 05:16 PHST- 2026/09/23 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:16 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - d6ra09208j [pii] AID - 10.1039/d6ra09208j [doi] PST - aheadofprint SO - RSC Adv. 2026 Oct 27. doi: 10.1039/d6ra09208j. PMID- 42948162 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1663-9812 (Print) IS - 1663-9812 (Electronic) IS - 1663-9812 (Linking) VI - 17 DP - 2026 TI - Organ-dependent phytochemical and pharmacological specialization in the endemic Adenocarpus decorticans Boiss. PG - 1919944 LID - 10.3389/fphar.2026.1919944 [doi] LID - 1919944 AB - INTRODUCTION: Adenocarpus decorticans Boiss. is a western Mediterranean shrub restricted to Morocco and Spain, whose phytochemistry and pharmacology remain unexplored despite ethnomedicinal records reported for other species of the genus. This study reports the first organ-resolved characterisation of the species. METHODS: Hydroethanolic extracts of flowers, leaves and stems were quantified for total phenolic, flavonoid and proanthocyanidin contents and profiled by ultra-high-performance liquid chromatography coupled with electrospray ionisation mass spectrometry in targeted selected-ion monitoring mode (UHPLC-ESI-MS/SIM). Radical-scavenging and reducing reactivity was determined by DPPH, ABTS and phosphomolybdenum assays. Antibacterial activity was assessed by disc diffusion with minimum inhibitory and bactericidal concentrations against four Gram-positive and two Gram-negative strains, and antiproliferative activity against P3X63Ag8.653 murine myeloma cells and normal splenocytes by MTT. Molecular docking, 100 ns molecular dynamics simulations and ADMET profiling were performed on the identified metabolites. RESULTS: Profiling revealed pronounced organ-dependent specialization: flowers were dominated by rosmarinic acid, leaves by flavonol and flavanone glycosides, and stems by procyanidin B2 and diverse flavones. Leaves showed the highest phenolic content (526.90 mg gallic acid equivalents/g extract) and the strongest radical-scavenging reactivity (DPPH IC(50) 18.62 mug/mL); flowers were the most active against the Gram-positive strains, with bactericidal activity at MIC 0.78-2.08 mg/mL; and stems were the most antiproliferative against P3 cells (IC(50) 342.66 mug/mL) with the highest selectivity index (1.97). Docking and dynamics identified flavonoid glycosides and proanthocyanidins as the most stable binders, the EGFR-amentoflavone complex being the most stable over 100 ns. DISCUSSION: These findings reveal clear organ-driven chemical and functional specialization in A. decorticans, consistent with the traditional medicinal use documented for the species. Because all assays were performed on crude extracts, the composition-activity correspondences identify candidate constituents rather than establish their contribution, and bioassay-guided fractionation and in vivo validation remain necessary. CI - Copyright (c) 2026 Elbirgui, Assioui, El Faqer, Harrizi, Zourif, Rafik, Conte, Habti, Elalami, Ennaji, Iounes and Ibenmoussa. FAU - Elbirgui, Kaouthar AU - Elbirgui K AD - Laboratory of Chemistry and Biochemistry, Nutrition, Environment and Health, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University of Casablanca, Casablanca, Morocco. AD - Laboratory of Ecology and Environment, Faculty of Sciences Ben M'Sick, Hassan II University of Casablanca, Casablanca, Morocco. FAU - Assioui, Houssam AU - Assioui H AD - Laboratory of Integrative Biology, Faculty of Sciences Ain Chock, Hassan II University of Casablanca, Casablanca, Morocco. FAU - El Faqer, Othman AU - El Faqer O AD - Laboratory of Integrative Biology, Faculty of Sciences Ain Chock, Hassan II University of Casablanca, Casablanca, Morocco. FAU - Harrizi, Saad AU - Harrizi S AD - Laboratory of Health and Environment and Biotechnology, Faculty of Sciences Ain Chock, Hassan II University of Casablanca, Casablanca, Morocco. FAU - Zourif, Ali AU - Zourif A AD - Laboratory of Physical-Chemistry, Materials and Catalysis, Faculty of Sciences Ben M'Sick, Hassan II University of Casablanca, Casablanca, Morocco. FAU - Rafik, Oussama AU - Rafik O AD - Laboratory of Ecology and Environment, Faculty of Sciences Ben M'Sick, Hassan II University of Casablanca, Casablanca, Morocco. FAU - Conte, Raffaele AU - Conte R AD - Research Institute on Terrestrial Ecosystems (IRET), National Research Council (CNR), Naples, Italy. FAU - Habti, Norddine AU - Habti N AD - Laboratory of Hematology, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University of Casablanca, Casablanca, Morocco. FAU - Elalami, Houda AU - Elalami H AD - Laboratory of Chemistry and Biochemistry, Nutrition, Environment and Health, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University of Casablanca, Casablanca, Morocco. FAU - Ennaji, Hanane AU - Ennaji H AD - Laboratory of Chemistry and Biochemistry, Nutrition, Environment and Health, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University of Casablanca, Casablanca, Morocco. FAU - Iounes, Nadia AU - Iounes N AD - Laboratory of Ecology and Environment, Faculty of Sciences Ben M'Sick, Hassan II University of Casablanca, Casablanca, Morocco. FAU - Ibenmoussa, Samir AU - Ibenmoussa S AD - Laboratory of Chemistry and Biochemistry, Nutrition, Environment and Health, Faculty of Medicine and Pharmacy of Casablanca, Hassan II University of Casablanca, Casablanca, Morocco. LA - eng PT - Journal Article DEP - 20261008 PL - Switzerland TA - Front Pharmacol JT - Frontiers in pharmacology JID - 101548923 PMC - PMC13711306 OTO - NOTNLM OT - Adenocarpus decorticans Boiss. OT - UHPLC-ESI-MS OT - antibacterial OT - antiproliferative OT - phytochemical profiling OT - radical-scavenging COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/08 CRDT- 2026/10/28 05:16 PHST- 2026/06/25 00:00 [received] PHST- 2026/07/29 00:00 [revised] PHST- 2026/08/05 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:16 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - 1919944 [pii] AID - 10.3389/fphar.2026.1919944 [doi] PST - epublish SO - Front Pharmacol. 2026 Oct 8;17:1919944. doi: 10.3389/fphar.2026.1919944. eCollection 2026. PMID- 42948161 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-462X (Print) IS - 1664-462X (Electronic) IS - 1664-462X (Linking) VI - 17 DP - 2026 TI - Hyperspectral multi-trait modeling for rapid assessment of rice photosynthetic light-response traits under field conditions. PG - 1968739 LID - 10.3389/fpls.2026.1968739 [doi] LID - 1968739 AB - Light-response-curve (LRC) derived photosynthetic phenotyping traits, including the maximum net photosynthetic rate (PNmax), light-saturation point (Isat), light-compensation point (Icomp), and dark respiration rate (Rd), are important indicators of photosynthetic performance. However, conventional LI-6400-based measurements require tens of minutes per leaf, severely limiting high-throughput phenotyping and field-based stress monitoring. Here, we developed a hyperspectral approach that simultaneously estimates four key LRC-derived photosynthetic traits (PNmax, Isat, Icomp, and Rd) from leaf hyperspectral data. The framework was evaluated using observations collected under field conditions encompassing three years, three rice varieties, four nitrogen levels, three irrigation treatments, three leaf positions, and multiple measurement dates. Partial Least Squares Regression (PLSR) and machine learning models-including Backpropagation Artificial Neural Network (BP-ANN), Convolutional Neural Networks (CNN), and Support Vector Regression (SVR)-were tested with full spectrum and variable selection methods (Competitive Adaptive Reweighted Sampling, CARS; Successive Projections Algorithm, SPA; and Uninformative Variable Elimination, UVE). Variable selection generally improved model performance, with CARS providing the most stable overall results across the four traits. Multi-trait (MT) models outperformed single-trait (ST) counterparts, particularly on the held-out test set. Among all methods, nonlinear models achieved higher accuracy for individual traits. PLSR2+CARS provided the most consistent overall predictive performance across the four traits, achieving test-set R(2) values of 0.84, 0.69, 0.52, and 0.59 and RMSE values of 2.53 mumol (CO(2)) m(-2) s(-1), 344.08 mumol (photon) m(-2) s(-1), 11.48 mumol (photon) m(-2) s(-1), and 0.58 mumol (CO(2)) m(-2) s(-1) for PNmax, Isat, Icomp, and Rd, respectively. These results indicate that leaf hyperspectral sensing combined with MT modeling has potential for high-throughput assessment of rice photosynthetic performance at the leaf level under field conditions. CI - Copyright (c) 2026 Yao, Zheng, Sun, Zhou, Wang and Li. FAU - Yao, Xinfeng AU - Yao X AD - Institute of Agricultural Science and Technology Information, Shanghai Academy of Agricultural Sciences, Shanghai, China. AD - Key Laboratory of Intelligent Agricultural Technology (Yangtze River Delta), Ministry of Agriculture and Rural Affairs, Shanghai, China. FAU - Zheng, Xiuguo AU - Zheng X AD - Institute of Agricultural Science and Technology Information, Shanghai Academy of Agricultural Sciences, Shanghai, China. AD - Key Laboratory of Intelligent Agricultural Technology (Yangtze River Delta), Ministry of Agriculture and Rural Affairs, Shanghai, China. FAU - Sun, Huifeng AU - Sun H AD - Eco-environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China. AD - Shanghai Engineering Research Centre of Low-carbon Agriculture (SERCLA), Shanghai, China. AD - Key Laboratory of Low-carbon Green Agriculture in Southeastern China, Ministry of Agriculture and Rural Affairs, Shanghai, China. FAU - Zhou, Sheng AU - Zhou S AD - Eco-environmental Protection Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai, China. AD - Shanghai Engineering Research Centre of Low-carbon Agriculture (SERCLA), Shanghai, China. AD - Key Laboratory of Low-carbon Green Agriculture in Southeastern China, Ministry of Agriculture and Rural Affairs, Shanghai, China. FAU - Wang, Wei AU - Wang W AD - Shanghai Spark-X Technology Co., Ltd., Shanghai, China. FAU - Li, Linyi AU - Li L AD - Institute of Agricultural Science and Technology Information, Shanghai Academy of Agricultural Sciences, Shanghai, China. AD - Key Laboratory of Intelligent Agricultural Technology (Yangtze River Delta), Ministry of Agriculture and Rural Affairs, Shanghai, China. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Plant Sci JT - Frontiers in plant science JID - 101568200 PMC - PMC13710402 OTO - NOTNLM OT - hyperspectral sensing OT - multi-trait prediction OT - photosynthetic traits OT - precision agriculture OT - rice COIS- Author WW was employed by company Shanghai Spark-X Technology Co., Ltd. The remaining author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:16 PHST- 2026/08/15 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:16 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fpls.2026.1968739 [doi] PST - epublish SO - Front Plant Sci. 2026 Oct 13;17:1968739. doi: 10.3389/fpls.2026.1968739. eCollection 2026. PMID- 42948159 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2251-6581 (Print) IS - 2251-6581 (Electronic) IS - 2251-6581 (Linking) VI - 25 IP - 2 DP - 2026 Dec TI - Effects of chiglitazar on glycemic, lipid, and hepatic outcomes: A systematic review and meta-analysis. PG - 303 LID - 10.1007/s40200-026-02119-8 [doi] LID - 303 AB - PURPOSE: Chiglitazar is a peroxisome proliferator-activated receptor pan-agonist with potential metabolic benefits. This systematic review and meta-analysis assessed its efficacy and safety versus placebo or active comparators in adults with metabolic disorders. METHODS: PubMed, Scopus, and Web of Science were searched up to August 2026 for studies assessing chiglitazar in adults with metabolic disorders. Outcomes were pooled via random-effects meta-analysis with 95% confidence intervals, alongside heterogeneity, subgroup, and sensitivity analyses. RESULTS: Five studies involving 1,457 participants were included. Chiglitazar reduced fasting glucose at 24 weeks (MD - 0.99 mmol/L, 95% CI - 1.75 to - 0.24). HbA1c decreased at 12 weeks (MD - 0.52% points, 95% CI - 0.68 to - 0.37), but its 24-week reduction was not statistically significant. Fasting insulin decreased at 12 weeks (SMD - 0.25, 95% CI - 0.39 to - 0.11), and HOMA-IR decreased at 12 and 24 weeks. Triglyceride levels decreased and HDL-C increased at both time points; total and LDL cholesterol increased at 12 weeks. At 12 weeks, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) decreased (MD - 4.44 U/L, 95% CI - 6.09 to - 2.79; and - 1.23 U/L, 95% CI - 2.28 to - 0.19, respectively). AST decreased at 24 weeks (MD - 3.03 U/L, 95% CI - 5.85 to - 0.21), whereas ALT did not significantly decrease (MD - 4.52 U/L, 95% CI - 9.34 to 0.30). No significant differences in overall or serious adverse events were detected; edema was increased (RR 8.77, 95% CI 1.60-47.97). CONCLUSION: Chiglitazar may improve short-term glycemic control, insulin sensitivity, selected lipid measures, and aminotransferase levels in adults with metabolic disorders. Increased edema risk, heterogeneity, and limited follow-up warrant cautious interpretation; long-term clinical benefits remain unproven. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at https://doi.org/10.1007/s40200-026-02119-8. CI - (c) The Author(s), under exclusive licence to Tehran University of Medical Sciences 2026. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. FAU - Altamimi, Abdulaziz AU - Altamimi A AUID- ORCID: 0009-0008-1715-0749 AD - College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia. ROR: https://ror.org/0149jvn88. GRID: grid.412149.b. ISNI: 0000 0004 0608 0662 FAU - Albegamy, Yousef H AU - Albegamy YH AD - College of Medicine, Imam Mohammad Ibn Saud Islamic University, Riyadh, Saudi Arabia. ROR: https://ror.org/05gxjyb39. GRID: grid.440750.2. ISNI: 0000 0001 2243 1790 FAU - Al Al Omar, Jehan AU - Al Al Omar J AD - Faculty of Medicine, Cairo University, Cairo, Egypt. ROR: https://ror.org/03q21mh05. GRID: grid.7776.1. ISNI: 0000 0004 0639 9286 FAU - Alkehili, Mohammed M AU - Alkehili MM AD - College of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia. ROR: https://ror.org/02ma4wv74. GRID: grid.412125.1. ISNI: 0000 0001 0619 1117 FAU - Alshajry, Bader Abdullah M AU - Alshajry BAM AD - College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia. ROR: https://ror.org/0149jvn88. GRID: grid.412149.b. ISNI: 0000 0004 0608 0662 FAU - Alruwaili, Musab H AU - Alruwaili MH AD - College of Medicine, Jouf University, Sakaka, Saudi Arabia. ROR: https://ror.org/02zsyt821. GRID: grid.440748.b. ISNI: 0000 0004 1756 6705 FAU - Alqahtani, Remas Mohammed AU - Alqahtani RM AD - College of Medicine, King Khaled University, Abha, Saudi Arabia. ROR: https://ror.org/052kwzs30. GRID: grid.412144.6. ISNI: 0000 0004 1790 7100 FAU - Alruqi, Farah K AU - Alruqi FK AD - Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia. ROR: https://ror.org/02ma4wv74. GRID: grid.412125.1. ISNI: 0000 0001 0619 1117 FAU - Alosemy, Majed F AU - Alosemy MF AD - Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia. ROR: https://ror.org/02ma4wv74. GRID: grid.412125.1. ISNI: 0000 0001 0619 1117 FAU - Alotaibi, Talal Mohammed AU - Alotaibi TM AD - College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia. ROR: https://ror.org/0149jvn88. GRID: grid.412149.b. ISNI: 0000 0004 0608 0662 FAU - Alkhulayfi, Mohammed Abdullah AU - Alkhulayfi MA AD - College of Medicine, King Saud University, Riyadh, Saudi Arabia. ROR: https://ror.org/02f81g417. GRID: grid.56302.32. ISNI: 0000 0004 1773 5396 FAU - Alhejaili, Afnan D AU - Alhejaili AD AD - College of Medicine, Taibah University, Madinah, Saudi Arabia. ROR: https://ror.org/01xv1nn60. GRID: grid.412892.4. ISNI: 0000 0004 1754 9358 FAU - Alanazi, Mansuor A AU - Alanazi MA AD - Diabetes and Chronic Diseases Unit, Faculty of Medicine, University of Tabuk, Tabuk, Saudi Arabia. ROR: https://ror.org/04yej8x59. GRID: grid.440760.1. ISNI: 0000 0004 0419 5685 LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Switzerland TA - J Diabetes Metab Disord JT - Journal of diabetes and metabolic disorders JID - 101590741 PMC - PMC13710484 OTO - NOTNLM OT - Chiglitazar OT - Diabetes mellitus, Type 2 OT - Fatty liver OT - Hypoglycemic agents OT - Insulin resistance OT - Lipids OT - Polyendocrine metabolic ovarian syndrome COIS- Competing interestsThe authors declare no competing interests. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2027/10/27 CRDT- 2026/10/28 05:16 PHST- 2026/10/07 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:16 [entrez] PHST- 2027/10/27 00:00 [pmc-release] AID - 2119 [pii] AID - 10.1007/s40200-026-02119-8 [doi] PST - epublish SO - J Diabetes Metab Disord. 2026 Oct 27;25(2):303. doi: 10.1007/s40200-026-02119-8. eCollection 2026 Dec. PMID- 42948158 OWN - NLM STAT- Publisher LR - 20261029 IS - 2046-2069 (Electronic) IS - 2046-2069 (Linking) DP - 2026 Oct 27 TI - New sulfonamide derivatives as HDAC inhibitors and apoptosis inducers: design, synthesis, in silico study and anticancer evaluation. LID - 10.1039/d6ra05385h [doi] AB - Cancer statistics indicate that more research is required to combat such a global fatal disease. In an attempt to develop an effective anticancer scaffold, we designed and synthesized new sulfonamide derivatives for potential histone deacetylase (HDAC) inhibition. The new derivatives showed antiproliferative activity against three cancer cell lines: HepG-2 (hepatoma), A549 (lung carcinoma), and MCF-7 (breast carcinoma). The most sensitive cancer cell line to the new derivatives was MCF-7, followed by A549 then HepG-2. Compound 10 emerged as the most effective candidate, especially against MCF-7, where it showed an IC(50) of 13.56 +/- 1.36 microM. Meanwhile, it had an IC(50) of 20.03 +/- 2.17 and 34.71 +/- 4.62 microM against A549 and HepG-2 cells, respectively. Interestingly, molecule 10 exhibited a markedly higher in vitro selectivity toward the tested cancer cell lines than the reference drug 5-fluorouracil (5-FU), with selectivity indices of 8.74 +/- 0.88, 5.92 +/- 0.74, and 3.41 +/- 0.39 against MCF-7, A549, and HepG-2 cells, respectively, compared with 1.59 +/- 0.13, 1.16 +/- 0.09, and 1.67 +/- 0.14 for 5-FU, respectively. These findings indicate greater in vitro selectivity of compound 10 toward the tested cancer cells relative to the normal WI-38 cell line; however, they should not be interpreted as evidence of superior overall safety compared with 5-FU. Furthermore, 10 caused inhibition to HDAC especially HDAC-2, showing an IC(50) of 2.27 +/- 0.07 microM, in comparison to the reference molecule entinostat (IC(50) of 1.21 +/- 0.04 microM). The Annexin V-FITC/PI assay indicated that apoptosis was the predominant mode of cell death caused by compound 10. Compound 10 caused an approximately 15-fold increase in the total number of apoptotic MCF-7 cells relative to untreated control cells. Meanwhile, compound 10 increased the expression levels of apoptotic markers p53, and the protein levels of caspase-9 in MCF-7 cells. Whereas it decreased the expression levels of Bcl-2 and VEGF. The docking data that revealed insights into the binding pattern of compound 10 to HDAC-2, were consistent with the biological data. Finally, this study suggests that compound 10 may serve as a lead molecule for further optimization toward new anticancer drugs. CI - This journal is (c) The Royal Society of Chemistry. FAU - Al Kamaly, Omkulthom AU - Al Kamaly O AUID- ORCID: 0000-0003-4706-4311 AD - Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University P.O. Box 84428 Riyadh 11671 Saudi Arabia. FAU - El-Fakharany, Esmail M AU - El-Fakharany EM AUID- ORCID: 0000-0002-0724-8477 AD - Protein Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City) New Borg Al-Arab City 21934 Alexandria Egypt. FAU - El-Maradny, Yousra A AU - El-Maradny YA AUID- ORCID: 0000-0001-9501-8059 AD - Protein Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City) New Borg Al-Arab City 21934 Alexandria Egypt. FAU - Alshabani, Lama A AU - Alshabani LA AUID- ORCID: 0009-0004-5460-3330 AD - Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University P.O. Box 84428 Riyadh 11671 Saudi Arabia. FAU - Alsfouk, Aisha AU - Alsfouk A AUID- ORCID: 0000-0003-4497-5013 AD - Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University P.O. Box 84428 Riyadh 11671 Saudi Arabia. FAU - Abdallah, Abdallah E AU - Abdallah AE AUID- ORCID: 0000-0003-2597-7419 AD - Pharmaceutical Medicinal Chemistry & Drug Design Department, Faculty of Pharmacy (Boys), Al-Azhar University Cairo 11884 Egypt abdulla_emara@azhar.edu.eg. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - RSC Adv JT - RSC advances JID - 101581657 PMC - PMC13711385 COIS- The authors have no competing interests relevant to this work to declare. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/27 CRDT- 2026/10/28 05:16 PHST- 2026/06/18 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:16 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - d6ra05385h [pii] AID - 10.1039/d6ra05385h [doi] PST - aheadofprint SO - RSC Adv. 2026 Oct 27. doi: 10.1039/d6ra05385h. PMID- 42948148 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-2646 (Print) IS - 2296-2646 (Electronic) IS - 2296-2646 (Linking) VI - 14 DP - 2026 TI - Cytochrome P450 inhibitors: friends or foes? PG - 1988344 LID - 10.3389/fchem.2026.1988344 [doi] LID - 1988344 AB - Cytochrome P450 (CYP450) enzymes play a central role in the metabolism of xenobiotics and endogenous compounds, influencing drug pharmacokinetics, efficacy, and safety. Inhibition of these enzymes has traditionally been viewed as a major liability in drug discovery due to its association with drug-drug interactions (DDIs), toxicity, and metabolic instability. However, inhibition of specific CYP450 isoforms has emerged as a powerful therapeutic strategy in multiple disease areas, including cancer and infectious diseases. This review presents a comprehensive analysis of data and regulatory guidance on CYP450 inhibitors to gain insights into whether CYP450 inhibition is a useful property ("a friend") or a liability ("a foe") for drug discovery scientists. Review of the literature shows that the design of novel CYP450 inhibitors requires an understanding of mechanisms of inhibition, whereas the clinical outcomes are mainly determined by isoform selectivity and effects of metabolism on drug action including reactive metabolite formation. The kinetic behaviour of representative inhibitor drugs is reassessed using variations in Michaels-Menten parameters beta = K (m)/K (i), and partitioning factor (k (inact)/k (off)). A modified classification of CYP450 inhibitors is proposed which consolidates the often-confusing classifications (mechanistic vs. spectroscopic) reported in the literature and FDA guidelines. The beneficial role of CYP450 inhibition in treatment and pharmacokinetic boosting is also discussed. Finally, advances utilising deep neural networks (DeepCYP) and machine learning methods (CYPlebrity, ML-augmented docking) in the last 5 years in inhibitor predictions are reviewed. This review provides a fresh perspective on classifying CYP450 inhibitors, existing guidelines for evaluation of in-vitro drug-drug interactions, and factors influencing clinical outcomes of CYP450 inhibition. This is expected to help researchers correctly identify CYP450 inhibitors as Friends or Foes in various discovery and clinical settings. CI - Copyright (c) 2026 Vyas, Ahmed and Dixit. FAU - Vyas, Shivam Kumar AU - Vyas SK AD - Department of Medicinal Chemistry, Department of Pharmaceuticals, Ministry of Chemicals and Fertilisers, National Institute of Pharmaceutical Education and Research, Guwahati (NIPER Guwahati), Govt. of India, Guwahati, Assam, India. FAU - Ahmed, Md Imran AU - Ahmed MI AD - Department of Medicinal Chemistry, Department of Pharmaceuticals, Ministry of Chemicals and Fertilisers, National Institute of Pharmaceutical Education and Research, Guwahati (NIPER Guwahati), Govt. of India, Guwahati, Assam, India. FAU - Dixit, Vaibhav A AU - Dixit VA AD - Department of Medicinal Chemistry, Department of Pharmaceuticals, Ministry of Chemicals and Fertilisers, National Institute of Pharmaceutical Education and Research, Guwahati (NIPER Guwahati), Govt. of India, Guwahati, Assam, India. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Chem JT - Frontiers in chemistry JID - 101627988 PMC - PMC13711265 OTO - NOTNLM OT - CYP450 OT - drug metabolism OT - inhibitor OT - irreversible inhibition kinetics OT - mechanism-based inhibition OT - partitioning factor COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:15 PHST- 2026/09/04 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:15 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 1988344 [pii] AID - 10.3389/fchem.2026.1988344 [doi] PST - epublish SO - Front Chem. 2026 Oct 13;14:1988344. doi: 10.3389/fchem.2026.1988344. eCollection 2026. PMID- 42948144 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2234-943X (Print) IS - 2234-943X (Electronic) IS - 2234-943X (Linking) VI - 16 DP - 2026 TI - Functional biomarkers complementing static BRCA/HRD testing for dynamic PARP inhibitor therapy in ovarian cancer: a narrative review. PG - 1989686 LID - 10.3389/fonc.2026.1989686 [doi] LID - 1989686 AB - Poly (ADP-ribose) polymerase inhibitors (PARPi) have revolutionized the management of high-grade serous ovarian cancer (HGSOC). Yet, current treatment decisions remain almost exclusively guided by static BRCA/homologous recombination deficiency (HRD) genotyping. This paradigm is increasingly challenged by a profound eligibility-benefit gap: real-world data show 93.4% of patients are PARPi-eligible, but only 22.0% achieve meaningful 2-year progression-free survival benefit, compounded by 10-30% HRD test failure rates, inability to capture dynamic resistance evolution, and negligible predictive value in the ~50% of HGSOC that are homologous recombination-proficient. This narrative review, based on literature up to June 2026, systematically evaluates functional biomarkers that complement BRCA/HRD testing by assessing real-time tumor biology. To our knowledge, this is the first review to integrate these biomarkers into a unified whole-disease-course framework accounting for evidence level, assay cost, and global resource setting. RAD51 immunohistochemistry directly visualizes homologous recombination repair capacity, achieving 95% accuracy in predicting PARPi response in preclinical patient-derived xenograft models and demonstrating robust predictive value in prospective clinical cohorts. The KELIM model, derived from early CA-125 kinetics and evaluated in >12,000 patients within prospective trial and registry datasets, provides a no-additional-assay-cost, tissue-independent readout of chemosensitivity with potential for broad, low-cost global deployment. Circulating tumor DNA enables noninvasive longitudinal monitoring of resistance, potentially preceding radiographic progression by weeks to months. Exploratory immune and artificial intelligence-based multimodal markers further refine response stratification. We propose deployment of these biomarkers at four critical clinical nodes-diagnosis, post-chemotherapy, maintenance, and progression-to guide therapy selection dynamically. By transitioning from static genotype-based eligibility to real-time functional phenotyping, this integrated strategy aims to close the eligibility-benefit gap and advance adaptive precision oncology for ovarian cancer globally. As a narrative review, this work aims to offer forward-looking conceptual frameworks and stimulate future research rather than to establish high-level clinical evidence or formal guidelines. CI - Copyright (c) 2026 Wu, Zhang, Xi, Wang, Hu, Shen, Tao, Lu and Wang. FAU - Wu, Tao AU - Wu T AD - Department of Gynecologic Cancer, Shaanxi Provincial Cancer Hospital, Xi'an, Shaanxi, China. FAU - Zhang, Pengchuang AU - Zhang P AD - Department of Gynecologic Cancer, Shaanxi Provincial Cancer Hospital, Xi'an, Shaanxi, China. FAU - Xi, Ruxing AU - Xi R AD - Department of Gynecologic Cancer, Shaanxi Provincial Cancer Hospital, Xi'an, Shaanxi, China. FAU - Wang, Xiaowei AU - Wang X AD - Department of Gynecologic Cancer, Shaanxi Provincial Cancer Hospital, Xi'an, Shaanxi, China. FAU - Hu, Lijuan AU - Hu L AD - Department of Gynecologic Cancer, Shaanxi Provincial Cancer Hospital, Xi'an, Shaanxi, China. FAU - Shen, Yahui AU - Shen Y AD - Department of Gynecologic Cancer, Shaanxi Provincial Cancer Hospital, Xi'an, Shaanxi, China. FAU - Tao, Kai AU - Tao K AD - Department of Gynecologic Cancer, Shaanxi Provincial Cancer Hospital, Xi'an, Shaanxi, China. FAU - Lu, Jianrong AU - Lu J AD - Department of Pathology, Shaanxi Provincial Cancer Hospital, Xi'an, Shaanxi, China. FAU - Wang, Guoqing AU - Wang G AD - Department of Gynecologic Cancer, Shaanxi Provincial Cancer Hospital, Xi'an, Shaanxi, China. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Oncol JT - Frontiers in oncology JID - 101568867 PMC - PMC13711281 OTO - NOTNLM OT - KELIM OT - PARP inhibitor OT - RAD51 OT - circulating tumor DNA OT - functional biomarker OT - minimal residual disease OT - ovarian cancer COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:15 PHST- 2026/09/05 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:15 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fonc.2026.1989686 [doi] PST - epublish SO - Front Oncol. 2026 Oct 13;16:1989686. doi: 10.3389/fonc.2026.1989686. eCollection 2026. PMID- 42948141 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-875X (Print) IS - 2296-875X (Electronic) IS - 2296-875X (Linking) VI - 13 DP - 2026 TI - Clinical efficacy of Delta large-channel endoscopy versus percutaneous endoscopic lumbar discectomy in treating far lateral lumbar disc herniation: a retrospective study. PG - 1902290 LID - 10.3389/fsurg.2026.1902290 [doi] LID - 1902290 AB - BACKGROUND: Far lateral lumbar disc herniation (FLLDH) accounts for 7%-12% of lumbar disc herniations, causing significant radicular pain, hypoesthesia, and muscle weakness. Due to its lateral location, traditional open surgery has limitations, including extensive tissue disruption and long recovery times. Minimally invasive techniques, such as The Delta large-channel endoscopy and percutaneous endoscopic lumbar discectomy (PELD), have emerged as alternatives, but there is limited comparative evidence on their efficacy for FLLDH. METHODS: This retrospective study included 78 patients with FLLDH, divided into two groups: 39 patients underwent the Delta large-channel endoscopy and 39 underwent PELD. The study compared both procedures in terms of operation time, blood loss, postoperative recovery, pain relief, functional improvement, and complications. All patients had failed conservative treatment and were diagnosed with FLLDH based on MRI and CT scans. RESULTS: Both surgical methods significantly improved pain and function. The Delta large-channel endoscopy group had longer operation times and more blood loss, but fewer fluoroscopies. Postoperative pain and functional recovery, assessed using VAS and ODI scores, were similar across both groups. The excellent/good rate based on the MacNab criteria was 92.31% for the Delta large-channel endoscopy and 84.62% for PELD, with no significant differences. CONCLUSIONS: Both Delta large-channel endoscopy and PELD effectively relieved pain and improved function in FLLDH patients. The Delta large-channel endoscopy, though requiring longer operation times, showed advantages in early recovery and more thorough decompression for complex cases. The choice of technique should depend on lesion characteristics and surgeon expertise. CI - (c) 2026 Xu, Zhao, Jiang and Yang. FAU - Xu, Yong AU - Xu Y AD - Department of Orthopedics, Zhenjiang First People's Hospital, Zhenjiang, Jiangsu, China. FAU - Zhao, Yilei AU - Zhao Y AD - Department of Orthopedics, Zhenjiang First People's Hospital, Zhenjiang, Jiangsu, China. FAU - Jiang, Qinyi AU - Jiang Q AD - Department of Orthopedics, Zhenjiang First People's Hospital, Zhenjiang, Jiangsu, China. FAU - Yang, Kangjia AU - Yang K AD - Department of Orthopedics, Zhenjiang First People's Hospital, Zhenjiang, Jiangsu, China. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Surg JT - Frontiers in surgery JID - 101645127 PMC - PMC13710398 OTO - NOTNLM OT - Delta large-channel endoscopy OT - far lateral lumbar disc herniation OT - minimally invasive surgery OT - percutaneous endoscopic lumbar discectomy OT - postoperative recovery COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:15 PHST- 2026/06/07 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:15 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fsurg.2026.1902290 [doi] PST - epublish SO - Front Surg. 2026 Oct 13;13:1902290. doi: 10.3389/fsurg.2026.1902290. eCollection 2026. PMID- 42948139 OWN - NLM STAT- Publisher LR - 20261029 IS - 1535-7597 (Print) IS - 1535-7511 (Electronic) IS - 1535-7511 (Linking) DP - 2026 Oct 26 TI - American Epilepsy Society/Congress of Neurological Surgeons Epilepsy Surgery Clinical Practice Guideline. PG - 15357597261465347 LID - 10.1177/15357597261465347 [doi] LID - 15357597261465347 AB - This practice guideline from the American Epilepsy Society (AES) and the Congress of Neurological Surgeons (CNS) provides evidence-based recommendations for surgical treatment of epilepsy for adults and children. It is an update to previous practice parameters published by the Academy of Neurology in 2003. The multidisciplinary panel addressed resective procedures for mesial temporal epilepsy (MTLE), neocortical epilepsy, and resection/disconnection of hypothalamic hamartoma. It also examined evidence for callosotomy in developmental and epileptic encephalopathies. It did not intentionally address neurostimulation treatment. A comprehensive literature review was conducted using the Grading of Recommendations Assessment, Development and Education (GRADE) methodology, and GRADE was also used for decision-making. This guideline considers not only seizure outcomes but also other critical and important outcomes. Seizures, quality of life, mortality, adverse events, and verbal memory loss were considered critical outcomes, while other neuropsychological deficits besides verbal memory loss (ie, naming, fluency), neuropsychiatric, and social outcomes were considered important outcomes. Following the GRADE process, the following strong recommendation was made based on a moderate level of evidence: (1) anterior temporal lobectomy (ATL) is recommended for people with MTLE at low risk for cognitive decline. Due to very low to low certainty of evidence the panel suggested the following conditional recommendations: (1) ATL for people with MTLE at high risk for cognitive decline, (2) selective amygdalo-hippocampectomy (SAH) in MTLE, (3) laser interstitial thermal therapy (LITT) in MTLE, (4) neocortical resection in neocortical epilepsy, and (5) callosotomy in people with drop attacks, Lennox-Gastaut Syndrome (LGS) or developmental and epileptic encephalopathies. The panel recognized that conditional recommendations need to account for individual patient circumstances and mandate shared decision-making to ensure that decisions align with each person's values and preferences. For hypothalamic hamartoma, the panel strongly recommended LITT despite low levels of evidence due to a paradigmatic situation where open surgery has significant risks that outweigh the benefits. If LITT is not available, the panel suggested radiosurgery for hypothalamic hamartoma (conditional recommendation). The panel highlighted that every person with drug-resistant epilepsy should have access to a tertiary epilepsy care center that performs surgical procedures and advanced epilepsy diagnostics (good practice statement). CI - (c) American Epilepsy Society and Congress of Neurological Surgeons 2026. FAU - Jobst, Barbara C AU - Jobst BC AUID- ORCID: 0000-0001-9243-2238 AD - Department of Neurology, Dartmouth Health and Geisel School of Medicine at Dartmouth, Lebanon, NH, USA. FAU - Bergey, Gregory AU - Bergey G AUID- ORCID: 0000-0001-7895-7564 AD - Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD, USA. FAU - Roldan-Benitez, Yetiani M AU - Roldan-Benitez YM AUID- ORCID: 0009-0009-4564-5973 AD - Department of Health Research Methods, Evidence, and Impact, McMaster University, Hamilton, ON, Canada. RINGGOLD: 3710 FAU - Hopp, Jennifer L AU - Hopp JL AUID- ORCID: 0000-0002-2326-0740 AD - Department of Neurology, University of Maryland School of Medicine, Baltimore, MD, USA. FAU - F Bauer, David AU - F Bauer D AUID- ORCID: 0000-0003-3548-0887 AD - Department of Neurosurgery, Department of Pediatric Neurosurgery, Baylor College of Medicine, Houston, TX, USA. RINGGOLD: 3984 FAU - Price, Angela AU - Price A AUID- ORCID: 0000-0001-8120-1055 AD - Department of Neurological Surgery, UT Southwestern Medical Center, Dallas, TX, USA. FAU - Strickland, Shawna L AU - Strickland SL AUID- ORCID: 0000-0003-2113-0835 AD - American Epilepsy Society and Department of Health Sciences, Rush University, Chicago, IL, USA. FAU - Bernat, James L AU - Bernat JL AUID- ORCID: 0000-0003-1128-0442 AD - Department of Neurology, Dartmouth Health and Geisel School of Medicine at Dartmouth, Hanover, NH, USA. FAU - Busch, Robyn M AU - Busch RM AD - Epilepsy Center and Department of Neurology, Neurological Institute, Cleveland Clinic, Cleveland, OH, USA. FAU - Engel, Jerome Jr AU - Engel J Jr AUID- ORCID: 0000-0001-6324-5716 AD - Department of Neurology, UCLA Medical Center, David Geffen School of Medicine, Los Angeles, CA, USA. FAU - Gloss, David AU - Gloss D AD - Department of Neurology, Neuromedical Center, Baton Rouge, LA, USA. FAU - Husain, Aatif M AU - Husain AM AUID- ORCID: 0000-0001-7954-1755 AD - Department of Neurology, Duke University Medical Center, Durham, NC, USA. FAU - McGovern, Robert A AU - McGovern RA AUID- ORCID: 0000-0003-0752-1899 AD - Neurosurgery Department, University of Minnesota, Minneapolis, MN, USA. RINGGOLD: 5635 FAU - McKhann, Guy M 2nd AU - McKhann GM 2nd AUID- ORCID: 0000-0002-9695-3564 AD - Department of Neurosurgery, Columbia University Irving Medical Center, New York, NY, USA. FAU - Nordli, Douglas R Jr AU - Nordli DR Jr AUID- ORCID: 0000-0002-3995-5467 AD - Department of Pediatric Neurology, University of Chicago, Chicago, IL, USA. FAU - Rolston, John D AU - Rolston JD AUID- ORCID: 0000-0002-8843-5468 AD - Department of Neurosurgery, Mass General Brigham, Harvard Medical School, Boston, MA, USA. FAU - Shane, Richard AU - Shane R AD - Patient representative, New York, NY, USA. FAU - Spencer, Dennis AU - Spencer D AD - Department of Neurosurgery, Yale School of Medicine, Yale University, New Haven, CT, USA. FAU - Weiner, Howard L AU - Weiner HL AD - Department of Neurosurgery, Baylor College of Medicine, Houston, TX, USA. RINGGOLD: 3984 FAU - Nasif, Mei Bou AU - Nasif MB AUID- ORCID: 0000-0003-2713-7894 AD - Neurology - Epilepsy, The George Washington University, Washington, DC, USA. RINGGOLD: 8367 FAU - Duda, Taylor AU - Duda T AD - Neurosurgery, Yale University, New Haven, CT, USA. FAU - Eid, Alexandra AU - Eid A AUID- ORCID: 0000-0001-9983-5793 AD - Department of Neurology, The George Washington University, Washington, DC, USA. FAU - Ghandour, Dina AU - Ghandour D AD - Department of Neurology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA. FAU - Hart, Shannon AU - Hart S AD - Department of Neurosurgery, McMaster University, Hamilton, ON, Canada. FAU - Kesserwan, Mohamad AU - Kesserwan M AUID- ORCID: 0000-0002-1593-0740 AD - Department of Neurosurgery, McMaster University, Hamilton, ON, Canada. FAU - Martyniuk, Amanda AU - Martyniuk A AD - Department of Surgery, McMaster University, Hamilton, ON, Canada. FAU - Lannon, Melissa AU - Lannon M AD - Division of Neurosurgery, McMaster University, Hamilton, ON, Canada. FAU - Saber, Golbarg T AU - Saber GT AUID- ORCID: 0000-0001-7310-583X AD - Department of Neurology, University of Chicago, Chicago, IL, USA. RINGGOLD: 2462 FAU - Saouda, Christopher AU - Saouda C AD - Department of Neurology, The George Washington University, Washington, DC, USA. FAU - Sharma, Sunjay AU - Sharma S AD - Department of Health Research Methods, Evidence, and Impact, McMaster University, Hamilton, ON, Canada. RINGGOLD: 3710 FAU - Vinarsky, Victoria AU - Vinarsky V AD - Department of Neurology, The George Washington University, Washington, DC, USA. FAU - J Darzi, Andrea AU - J Darzi A AUID- ORCID: 0000-0002-2498-1697 AD - Department of Health Research Methods, Evidence, and Impact, McMaster University, Hamilton, ON, Canada. RINGGOLD: 3710 AD - Department of Health Research Methods, Evidence, and Impact, Department of Anesthesia, McMaster University, Hamilton, ON, Canada. FAU - Gonzalez-Martinez, Jorge AU - Gonzalez-Martinez J AUID- ORCID: 0000-0002-9936-7034 AD - Department of Neurological Surgery, University of Pittsburgh, Pittsburgh, PA, USA. LA - eng PT - Journal Article PT - Review DEP - 20261026 PL - United States TA - Epilepsy Curr JT - Epilepsy currents JID - 101135954 PMC - PMC13710471 OTO - NOTNLM OT - anterior temporal lobectomy OT - corpus callosotomy OT - drug-resistant epilepsy OT - epilepsy surgery OT - hypothalamic hamartoma OT - laser interstitial thermal therapy OT - mesial temporal lobe epilepsy OT - neocortical epilepsy OT - selective amygdalohippocampectomy COIS- The authors declared the following potential conflicts of interest with respect to the research, authorship, and/or publication of this article: Elsevier Publishing (GB, RMB), Springer (RMB), Cleveland Clinic Foundation (RMB), Ceraxis Health (RMB), UpToDate Inc. (JLH), J Kiffin Penry (JLH), Marinus Pharmaceuticals (AMH), LivaNova USA, Inc. (AMH), Merck (AMH), Jazz Pharmaceuticals (AMH), EISAI, Inc. (AMH), PranaQ (AMH), UniQURE (AMH), NEUROPACE, Inc. (BCJ, JDR), Monteris Medical Corporation (RM), SynerFuse (RM), Koh Young (GM), Endostream Medical (GM), NeuroOne Medical Technologies (RM), Synchron (RM), Medtronic (RM), ClearPoint Medical (JDR), Turing Medical (JDR), The other authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/26 CRDT- 2026/10/28 05:14 PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:14 [entrez] PHST- 2026/10/26 00:00 [pmc-release] AID - 10.1177_15357597261465347 [pii] AID - 10.1177/15357597261465347 [doi] PST - aheadofprint SO - Epilepsy Curr. 2026 Oct 26:15357597261465347. doi: 10.1177/15357597261465347. PMID- 42948135 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Sodium oxybate with reduced-dose propofol for anesthesia induction in elective spine surgery: a randomized trial. PG - 1970835 LID - 10.3389/fmed.2026.1970835 [doi] LID - 1970835 AB - BACKGROUND: Sodium oxybate has an established sedative action, but its contribution to contemporary propofol-based anesthesia remains incompletely characterized. We evaluated induction hemodynamics, propofol use, bispectral index (BIS), and early recovery in elective spine surgery. METHODS: In this single-center randomized trial, 127 patients received propofol alone (P; n = 62) or propofol plus sodium oxybate (PS; n = 65). Planned induction doses were propofol 2.2 versus 1.2 mg/kg, with oxybate 30 mg/kg in PS; recorded propofol doses were lower in both groups. The primary outcome was the mean arterial pressure (MAP) trajectory across 13 induction landmarks. Maximum-likelihood mixed models were supplemented by unstructured-covariance and baseline-adjusted analyses. RESULTS: The primary group-by-time interaction was not significant (P = 0.607; unstructured covariance, P = 0.719). The baseline-adjusted average post-baseline MAP difference was 1.7 mmHg (PS minus P; 95% confidence interval [CI], -2.0-5.5). Systolic-pressure-defined hypotension occurred in 37/62 versus 32/65 patients (risk ratio, 0.82; 95% CI, 0.60-1.14; risk difference, -10.4 percentage points; 95% CI, -26.8-6.7). Recorded total propofol use was 31.4% lower (751.3 versus 515.4 mg; difference, -235.9 mg; 95% CI, -366.0 to -105.9). Maintenance use was also lower under unblinded BIS-guided titration. Post-intubation BIS <40 occurred in 2/62 versus 22/65 patients. The mean surgery-end-to-extubation interval was 10.5 versus 13.6 min (difference, 3.1 min; 95% CI, 0.9-5.2), although both medians were 10 min. Vasoactive-treatment documentation was incomplete. CONCLUSIONS: The oxybate-containing strategy was associated with substantially lower propofol use, lower BIS values, and a longer mean extubation interval; hemodynamic superiority was not demonstrated. These findings define the observed effects and trade-offs of the combined regimen under BIS-guided care and inform further evaluation of dose selection, hypnosis monitoring, and recovery. CLINICAL TRIAL REGISTRATION: https://www.chictr.org.cn Chinese Clinical Trial Registry, ChiCTR2400080686. CI - (c) 2026 Yu, Yu, Wu, Zhao, Xia and Fan. FAU - Yu, Jianfeng AU - Yu J AD - Department of Anesthesiology, Tsinghua University Yuquan Hospital (Tsinghua University Hospital of Integrated Traditional Chinese and Western Medicine), Beijing, China. FAU - Yu, Cuiping AU - Yu C AD - Department of Anesthesiology, Tsinghua University Yuquan Hospital (Tsinghua University Hospital of Integrated Traditional Chinese and Western Medicine), Beijing, China. FAU - Wu, Nanling AU - Wu N AD - Department of Anesthesiology, Tsinghua University Yuquan Hospital (Tsinghua University Hospital of Integrated Traditional Chinese and Western Medicine), Beijing, China. FAU - Zhao, Kaixuan AU - Zhao K AD - Department of Anesthesiology, Tsinghua University Yuquan Hospital (Tsinghua University Hospital of Integrated Traditional Chinese and Western Medicine), Beijing, China. FAU - Xia, Yimeng AU - Xia Y AD - Department of Anesthesiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Fan, Ting AU - Fan T AD - Department of Anesthesiology, Tsinghua University Yuquan Hospital (Tsinghua University Hospital of Integrated Traditional Chinese and Western Medicine), Beijing, China. LA - eng PT - Clinical Trial PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13710391 OTO - NOTNLM OT - anesthesia induction OT - bispectral index OT - gamma-hydroxybutyrate OT - hypotension OT - propofol OT - randomized controlled trial OT - sodium oxybate OT - spine surgery COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:14 PHST- 2026/08/18 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:14 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fmed.2026.1970835 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 13;13:1970835. doi: 10.3389/fmed.2026.1970835. eCollection 2026. PMID- 42948124 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2168-8184 (Print) IS - 2168-8184 (Electronic) IS - 2168-8184 (Linking) VI - 18 IP - 9 DP - 2026 Sep TI - Combined Transarterial Embolization and Percutaneous CT-Guided Cryoablation of Renal Cell Carcinoma: A Retrospective Single-Center Study. PG - e116993 LID - 10.7759/cureus.116993 [doi] LID - e116993 AB - Purpose The aim of this study was to evaluate the feasibility, safety, and oncological outcomes of combined transarterial embolization followed by percutaneous CT-guided cryoablation for renal cell carcinoma (RCC). Material and methods This retrospective single-center study included 24 patients with 24 biopsy-proven RCC who underwent transarterial embolization followed by percutaneous CT-guided cryoablation between May 2019 and August 2025. Procedural parameters, tumor characteristics, and oncological outcomes, as well as overall survival (OS), were assessed and analyzed. Results The mean tumor diameter was 3.9 +/- 1.4 cm, and the mean ablation time was 26 +/- 4.9 minutes. No major peri-procedural complications were observed. Early post-procedural imaging demonstrated initial complete ablation in 23 out of 24 tumors, equating to a rate of 95.8%. The rate of local tumor progression was 8.3% (2/24) and the rate of distant progression was 12.5% (3/24), which all occurred in patients without pre-existing metastatic disease. None of the patients with pre-existing metastatic disease developed local or distant disease progression during follow-up. The mean OS was 3.1 years (95% CI: 2.4-3.7) with a one- and two-year OS rate of 95% and 69.1%, respectively. Conclusion Combined transarterial embolization followed by percutaneous CT-guided cryoablation appears to be a safe and feasible treatment approach for selected patients with RCC, particularly those with limited surgical options. CI - Copyright (c) 2026, Vogl et al. FAU - Vogl, Thomas J AU - Vogl TJ AD - Radiology and Nuclear Medicine, Goethe University, University Hospital Frankfurt, Frankfurt, DEU. FAU - Recoschewitz, Catharina AU - Recoschewitz C AD - Radiology and Nuclear Medicine, Goethe University, University Hospital Frankfurt, Frankfurt, DEU. FAU - Cojocaru, Ruxandra AU - Cojocaru R AD - Radiology and Nuclear Medicine, Goethe University, University Hospital Frankfurt, Frankfurt, DEU. FAU - Nica, Andreea-Ioana AU - Nica AI AD - Radiology and Nuclear Medicine, Goethe University, University Hospital Frankfurt, Frankfurt, DEU. FAU - Adwan, Hamzah AU - Adwan H AD - Radiology and Nuclear Medicine, Goethe University, University Hospital Frankfurt, Frankfurt, DEU. LA - eng PT - Journal Article DEP - 20260927 PL - United States TA - Cureus JT - Cureus JID - 101596737 PMC - PMC13711298 OTO - NOTNLM OT - embolization OT - image-guided ablation OT - interventional oncology OT - minimally invasive therapy OT - percutaneous cryoablation OT - renal cell carcinoma OT - thermal ablation COIS- Human subjects: Informed consent for treatment and open access publication was obtained or waived by all participants in this study. Ethics Committee of the Faculty of Medicine at Goethe University, Frankfurt issued approval 2025-2714. Animal subjects: All authors have confirmed that this study did not involve animal subjects or tissue. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/09/27 CRDT- 2026/10/28 05:13 PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:13 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - 10.7759/cureus.116993 [doi] PST - epublish SO - Cureus. 2026 Sep 27;18(9):e116993. doi: 10.7759/cureus.116993. eCollection 2026 Sep. PMID- 42948123 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-2295 (Electronic) IS - 1664-2295 (Linking) VI - 17 DP - 2026 TI - A 2-year multimodal prospective cohort study to predict sustained progression independent of relapse activity in multiple sclerosis. PG - 1902455 LID - 10.3389/fneur.2026.1902455 [doi] LID - 1902455 AB - BACKGROUND: Disability accrual in multiple sclerosis (MS) is heterogeneous. We assessed whether a 2-year multimodal evaluation could identify patients who were developing progression independent of relapse activity (PIRA) or relapse-associated worsening (RAW). METHODS: Among the 81 MS patients with a 2-year follow-up and outcomes determined through 5 years, a composite for PIRA was developed using Firth's penalized logistic regression analysis; discrimination was calculated as the area under the curve (AUC) using repeated stratified nested cross-validation. RESULTS: In total, 21 patients (25.9%) met the PIRA criteria, 9 (11.1%) met the RAW criteria, and 1 met both criteria. A multivariate Firth regression analysis selected two variables associated with PIRA after adjustment: Modified Fatigue Impact Scale (MFIS) total score (OR 2.04, 95% CI 1.17-3.83; p = 0.011) and cerebrospinal fluid (CSF) chitinase-3-like protein 1 (CHI3L1) (OR 2.00, 95% CI 1.11-5.26; p = 0.018). Notably, the Symbol Digit Modalities Test (DeltaSDMT) showed an association in the univariate analysis (OR 0.47, 95% CI 0.23-0.82; p = 0.006) but was attenuated in the multivariate analysis [odds ratio (OR) 0.64; p = 0.141]. Oligoclonal immunoglobulin G band (OCB) positivity was universal in PIRA, but the findings are inconclusive due to sparse OCB-negative observations and incomplete OCB data. Worsening gait [DeltaTimed 25-Foot Walk (DeltaT25FW)] and new T2 lesions showed associations in the univariate analysis that were not retained in the multivariate analysis. None of the variables had a p-value of less than 0.10 for RAW. The median out-of-sample AUC was 0.74 (95% CI 0.68-0.78). CONCLUSION: A two-year multimodal assessment identified a biologically coherent PIRA composite driven by intrathecal neuroinflammation and fatigue. No reliable RAW predictor was found, and external validation is warranted. CI - Copyright (c) 2026 Solis-Tarazona, Reddam, Merchan-Cabanillas, Navarro-Quevedo, Raket and Gil-Perotin. FAU - Solis-Tarazona, Luis Rafael AU - Solis-Tarazona LR AD - Research Group in Immunotherapy and Biomodels for Autoimmunity, Instituto de Investigacion Sanitaria La Fe, Valencia, Spain. FAU - Reddam, Salma AU - Reddam S AD - Research Group in Immunotherapy and Biomodels for Autoimmunity, Instituto de Investigacion Sanitaria La Fe, Valencia, Spain. FAU - Merchan-Cabanillas, Ana AU - Merchan-Cabanillas A AD - Department of Neurology, Hospital Dr. Peset, Valencia, Spain. FAU - Navarro-Quevedo, Silvia AU - Navarro-Quevedo S AD - Department of Neurology, Hospital Universitario Vega Baja, Alicante, Spain. FAU - Raket, Lars Lau AU - Raket LL AD - Clinical Memory Research Unit, Department of Clinical Sciences, Lund University, Lund, Sweden. FAU - Gil-Perotin, Sara AU - Gil-Perotin S AD - Research Group in Immunotherapy and Biomodels for Autoimmunity, Instituto de Investigacion Sanitaria La Fe, Valencia, Spain. AD - Division of Hematology and Oncology, Department of Medicine, Columbia Initiative in Cell Engineering and Therapy (CICET), Columbia University Irving Medical Center, New York, NY, United States. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Neurol JT - Frontiers in neurology JID - 101546899 RN - 0 (Chitinase-3-Like Protein 1) RN - 0 (CHI3L1 protein, human) SB - IM MH - Humans MH - Female MH - *Disease Progression MH - Male MH - Adult MH - Prospective Studies MH - *Chitinase-3-Like Protein 1/cerebrospinal fluid MH - Middle Aged MH - Recurrence MH - *Multiple Sclerosis/cerebrospinal fluid/diagnosis/physiopathology MH - Disability Evaluation MH - Fatigue MH - Follow-Up Studies PMC - PMC13710352 OTO - NOTNLM OT - CHI3L1 OT - MFIs OT - PIRA OT - biomarkers OT - fatigue OT - multimodal OT - multiple sclerosis OT - smoldering MS COIS- LS-T is a full-time employee of Lundbeck A/S. LR is a full-time employee of Eli Lilly and Company. Their work on this publication is unrelated to LS-T and LR affiliations with these companies and is not subject to any commercial interest. SG-P has received speaker honoraria from Sanofi, Roche, Merck, and Biogen in the past and has participated in Merck and Bristol-Myers Squibb advisory boards. The remaining author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author SG-P declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:13 PHST- 2026/06/07 00:00 [received] PHST- 2026/09/07 00:00 [revised] PHST- 2026/09/14 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:13 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fneur.2026.1902455 [doi] PST - epublish SO - Front Neurol. 2026 Oct 13;17:1902455. doi: 10.3389/fneur.2026.1902455. eCollection 2026. PMID- 42948115 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2674-0850 (Electronic) IS - 2674-0850 (Linking) VI - 6 DP - 2026 TI - Maleimide availability in thiol-maleimide peptide conjugation on PEGylated liposomes: a reactive-site accounting framework illustrated using hepatic drug-delivery systems. PG - 1964437 LID - 10.3389/fddev.2026.1964437 [doi] LID - 1964437 AB - Peptide-targeted PEGylated liposomes are a widely investigated active-targeting strategy for hepatocellular carcinoma (HCC) and other hepatic indications. A critical but underreported formulation variable is effective maleimide availability: the number of outward-facing, unhydrolysed, sterically accessible maleimide groups present when peptide thiols are coupled. In the seven hepatic systems examined, authors often report nominal lipid or peptide input without measuring reactive sites, conjugation efficiency, or final surface density. Hydrolysis, steric access, bilayer orientation, and peptide-thiol oxidation are individually well-established features of thiol-maleimide chemistry; the contribution of this Perspective is not to identify these phenomena but to integrate them into a quantitative accounting framework that links nominal input to biological outcome. We therefore propose a reactive-site accounting framework that separates six distinct quantities often conflated in practice: nominal maleimide input (the formulation's stated lipid composition), reactive maleimide (the outward-facing, unhydrolyzed fraction actually present at coupling), available peptide thiol (the free-thiol fraction remaining after synthesis, reduction or thiolation, and handling), conjugation efficiency (the proportion of input peptide that is actually conjugated), final conjugated ligand density (molecules per particle), and biologically accessible ligand density (the fraction capable of engaging its receptor in the biological milieu). Route selection, such as distinguishing maleimide-lipid pre-incorporation, post-insertion of a lipid conjugate, and direct coupling on preformed liposomes, should be evaluated experimentally rather than assumed. The chemical limitations underlying this framework are not specific to hepatic targeting; the seven hepatic systems examined here serve as an illustrative case set for a broader reporting problem in thiol-maleimide functionalization of nanocarriers. Reporting these measures would make density-response relationships easier to interpret and peptide-liposome studies, hepatic and otherwise, easier to reproduce. CI - Copyright (c) 2026 Maroof and Genc. FAU - Maroof, Kashif AU - Maroof K AD - SUNUM, Sabanci University Nanotechnology Research Centre, Sabanci University, Istanbul, Turkiye. FAU - Genc, Rukan AU - Genc R AD - SUNUM, Sabanci University Nanotechnology Research Centre, Sabanci University, Istanbul, Turkiye. AD - Department of Chemical Engineering Faculty of Engineering Mersin University, Mersin, Turkiye. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Drug Deliv JT - Frontiers in drug delivery JID - 9918469589006676 PMC - PMC13710358 OTO - NOTNLM OT - DSPE-PEG-maleimide OT - conjugation efficiency OT - hepatic nanomedicine OT - hepatocellular carcinoma OT - maleimide availability OT - peptide-targeted liposomes OT - thiol-maleimide conjugation COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:12 PHST- 2026/08/11 00:00 [received] PHST- 2026/09/05 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:12 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 1964437 [pii] AID - 10.3389/fddev.2026.1964437 [doi] PST - epublish SO - Front Drug Deliv. 2026 Oct 13;6:1964437. doi: 10.3389/fddev.2026.1964437. eCollection 2026. PMID- 42948107 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-4185 (Print) IS - 2296-4185 (Electronic) IS - 2296-4185 (Linking) VI - 14 DP - 2026 TI - UV-derived scattering as a high-throughput readout for adeno-associated virus-like particle separation screenings. PG - 1913731 LID - 10.3389/fbioe.2026.1913731 [doi] LID - 1913731 AB - Virus-like particles (VLPs), including those derived from adeno-associated virus (AAV), are increasingly investigated for vaccination, capsid engineering, and gene-delivery applications. Although precipitation and aqueous two-phase separation (ATPS) represent promising alternatives to adsorption-based capture, their optimization is limited by the lack of rapid analytical methods suitable for complex process samples. Here, VP3-only AAV5 VLPs produced in Escherichia coli were used as a model system to evaluate UV-derived light scattering as a high-throughput separation readout. Blank-corrected absorbance at 320 nm was used as a relative descriptor of particulate-associated scattering, while scattering-corrected absorbance at 280 nm monitored the soluble biomolecular fraction. During PEG precipitation, A320 showed condition-dependent agreement with anti-AAV5 ELISA, with the closest correspondence for PEG4000 and increasing divergence at lower PEG molecular weights. Capsid-spiking experiments further showed that dispersed intact capsids alone contributed only marginally to A320, indicating that the signal primarily reflects larger scattering-active structures accompanying capsid depletion. The workflow enabled screening and analysis of 170 precipitation and PEG/phosphate ATPS conditions in biological duplicate within 3 days. Low-molecular-weight PEG systems provided stronger separation between soluble biomolecular material and particulate-associated scattering. In PEG400 ATPS, pH altered the distribution of scattering-active material and capsids between the liquid phases and interphase, whereas selected precipitation conditions showed higher recovery of assembled capsids after resolubilization than ATPS, at the expense of lower impurity removal. Overall, UV-derived scattering provides a rapid, scalable first-line readout for comparing separation conditions and reducing the number of samples requiring capsid-specific and structural characterization. CI - Copyright (c) 2026 Polazzo, Schwarzer and Hubbuch. FAU - Polazzo, Giulia AU - Polazzo G AD - Institute of Process Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany. FAU - Schwarzer, Malte AU - Schwarzer M AD - Institute of Process Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany. FAU - Hubbuch, Jurgen AU - Hubbuch J AD - Institute of Process Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Bioeng Biotechnol JT - Frontiers in bioengineering and biotechnology JID - 101632513 PMC - PMC13710323 OTO - NOTNLM OT - UV/Vis absorbance OT - adeno-associated virus (AAV) OT - aqueous two-phase system (ATPS) OT - biomolecular separation OT - downstream processing (DSP) OT - high-throughput screening OT - light scattering OT - virus-like particle (VLP) COIS- JH is a co-inventor of the SpecPlate. The remaining author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The reviewer TG declared past publications with the author JH at the time of review. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:12 PHST- 2026/06/19 00:00 [received] PHST- 2026/09/06 00:00 [revised] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:12 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 1913731 [pii] AID - 10.3389/fbioe.2026.1913731 [doi] PST - epublish SO - Front Bioeng Biotechnol. 2026 Oct 13;14:1913731. doi: 10.3389/fbioe.2026.1913731. eCollection 2026. PMID- 42948095 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2666-2736 (Electronic) IS - 2666-2736 (Linking) VI - 33 DP - 2026 Oct TI - Practice patterns and outcomes of local and systemic therapy for single-site metastatic non-small cell lung cancer in the era of immunotherapy. PG - 101949 LID - 10.1016/j.xjon.2026.101949 [doi] LID - 101949 AB - OBJECTIVE: Prognosis of lung cancer with single metastatic sites in the era of immunotherapy remains poorly described. We used the National Cancer Database to assess how metastatic site and the use of local therapy with systemic immunotherapy is associated with prognosis in the United States. METHODS: Patients with clinical stage IVA lung cancer treated with immunotherapy were identified in the National Cancer Database (2018-2023). Patients were stratified by metastatic site and treatment: systemic immunotherapy with or without local therapy (immunotherapy + local therapy or immunotherapy). Prognosis was evaluated using Kaplan-Meier and Cox survival analysis landmarked at median time from diagnosis to local therapy. RESULTS: Among included patients (N = 20,214), metastases included lung (N = 5308; 26.3%), brain (N = 3516; 17.4%), bone (N = 3142; 15.5%), distant lymph node (N = 1219; 6.0%), liver (N = 874; 4.3%), and other metastases (N = 6155; 30.4%). A total of 13,890 patients (68.7%) were treated with immunotherapy, and 6324 patients (31.3%) were treated with immunotherapy + local therapy (70.2% received chemotherapy, 27.2% radiation, and 9.8% surgery). Poor 5-year overall survival was associated with bone (20.9%) and liver (15.9%) metastases compared with brain (33.4%). Five-year survival was greater in patients selected for local therapy (30.3% vs 20.5%, P < .001); thoracic and extrathoracic local therapy was associated with greater overall survival compared with extrathoracic local therapy alone (37.7% vs 28.2%, P < .001). Differences in 5-year survival with local therapy varied by metastatic site (10.4% lung, 7.1% brain, 0.1% liver). CONCLUSIONS: The addition of local therapy to a systemic regimen containing immunotherapy was common for patients with single metastatic sites. Patients selected for local therapy had a more favorable prognosis, which varied by metastatic site. CI - (c) 2026 The Author(s). FAU - Esnaola, Gabriela AU - Esnaola G AD - Division of Thoracic Surgery, Department of Surgery, Yale School of Medicine, New Haven, Conn. FAU - Canavan, Maureen AU - Canavan M AD - Division of Thoracic Surgery, Department of Surgery, Yale School of Medicine, New Haven, Conn. FAU - Caturegli, Giorgio AU - Caturegli G AD - Division of Thoracic Surgery, Department of Surgery, Yale School of Medicine, New Haven, Conn. FAU - Lynch, Abigail AU - Lynch A AD - Division of Thoracic Surgery, Department of Surgery, Yale School of Medicine, New Haven, Conn. FAU - Resio, Benjamin AU - Resio B AD - Division of Thoracic Surgery, Department of Surgery, Yale School of Medicine, New Haven, Conn. LA - eng PT - Journal Article DEP - 20260702 PL - Netherlands TA - JTCVS Open JT - JTCVS open JID - 101768541 PMC - PMC13711191 OTO - NOTNLM OT - local therapy OT - non-small cell lung cancer OT - oligometastatic disease COIS- The authors reported no conflicts of interest. The Journal policy requires editors and reviewers to disclose conflicts of interest and to decline handling or reviewing manuscripts for which they may have a conflict of interest. The editors and reviewers of this article have no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/07/02 CRDT- 2026/10/28 05:11 PHST- 2026/05/07 00:00 [received] PHST- 2026/06/11 00:00 [revised] PHST- 2026/06/28 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:11 [entrez] PHST- 2026/07/02 00:00 [pmc-release] AID - S2666-2736(26)00372-4 [pii] AID - 101949 [pii] AID - 10.1016/j.xjon.2026.101949 [doi] PST - epublish SO - JTCVS Open. 2026 Jul 2;33:101949. doi: 10.1016/j.xjon.2026.101949. eCollection 2026 Oct. PMID- 42948085 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2666-2736 (Electronic) IS - 2666-2736 (Linking) VI - 33 DP - 2026 Oct TI - Contemporary chest drain practice after pulmonary resection: A multicenter registry study of drain strategy, outcomes, and center-level variation. PG - 102053 LID - 10.1016/j.xjon.2026.102053 [doi] LID - 102053 AB - OBJECTIVES: Chest drain management after pulmonary resection varies widely, particularly with respect to drain size, use of digital systems, and suction strategy. Although smaller drains and digital drainage systems are increasingly adopted, robust real-world data evaluating their impact on clinically relevant outcomes are limited. Using a large contemporary multicenter registry, we evaluated associations between chest drain characteristics and postoperative outcomes, and quantified center-level variation in drain practice. METHODS: A prospective multicenter observational registry including 3348 patients undergoing pulmonary resection across 21 international centers was analyzed. Patient, disease, operative, and drain-related variables were recorded. Primary outcome was prolonged air leak (>5 days). Secondary outcomes included pulmonary complications, surgical emphysema, pleural readmission, length of stay, drain duration, and in-hospital mortality. Multivariable regression models adjusted for patient, operative, and center-level factors were constructed. Center-level variation in drain practice and outcomes was examined descriptively and using funnel plots. RESULTS: The cohort comprised 3348 patients, of whom 88% underwent minimally invasive resection. Prolonged air leak occurred in 9.0%, pulmonary complications in 7%, and in-hospital mortality in 0.9%. Larger drain size was independently associated with lower odds of prolonged air leak (adjusted odds ratio per 1Fr increase approximately 1.12), whereas digital drainage systems were associated with higher detected prolonged air leak but shorter length of stay. Substantial center-level variation was observed in digital drain adoption, median drain size, and prolonged air leak rates, exceeding expected random variation in several centers. Reanalysis incorporating 3 additional international centers demonstrated no material change in adjusted effect estimates. CONCLUSIONS: In this large contemporary registry, chest drain strategy was independently associated with postoperative outcomes after pulmonary resection. Marked intercenter variation in drain practice persists, underscoring the need for evidence-based standardization. These real-world data provide a platform to rationalize chest drain management and inform future pragmatic trials. CI - (c) 2026 The Author(s). FAU - Patel, Akshay J AU - Patel AJ AD - Department of Thoracic Surgery, Guy's Hospital, Guy's and St Thomas' NHS Foundation Trust, London, England, United Kingdom. AD - College of Medical and Dental Sciences, Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, England, United Kingdom. FAU - Weedle, Rebecca AU - Weedle R AD - Department of Thoracic Surgery, St James' Hospital, Dublin, Ireland. FAU - Al-Shammari, Abdullah AU - Al-Shammari A AD - Department of Thoracic Surgery, Guy's Hospital, Guy's and St Thomas' NHS Foundation Trust, London, England, United Kingdom. FAU - Choi, Jeesoo AU - Choi J AD - Department of Thoracic Surgery, Guy's Hospital, Guy's and St Thomas' NHS Foundation Trust, London, England, United Kingdom. FAU - Bille, Andrea AU - Bille A AD - Department of Thoracic Surgery, Guy's Hospital, Guy's and St Thomas' NHS Foundation Trust, London, England, United Kingdom. CN - ESTS Drainology Collaborative LA - eng PT - Journal Article DEP - 20260813 PL - Netherlands TA - JTCVS Open JT - JTCVS open JID - 101768541 PMC - PMC13711205 OTO - NOTNLM OT - chest drain management OT - chest tube size OT - digital drainage systems OT - enhanced recovery after surgery OT - length of stay OT - prolonged air leak OT - pulmonary resection COIS- The authors reported no conflicts of interest. The Journal policy requires editors and reviewers to disclose conflicts of interest and to decline handling or reviewing manuscripts for which they may have a conflict of interest. The editors and reviewers of this article have no conflicts of interest. FIR - Ruffini, Enrico IR - Ruffini E FIR - Guerrera, Francesco IR - Guerrera F FIR - Caffarotti, Stefano IR - Caffarotti S FIR - Patella, Miriam IR - Patella M FIR - Fallouh, Hazem IR - Fallouh H FIR - Girotti, Paolo IR - Girotti P FIR - Weedle, Rebecca IR - Weedle R FIR - Al-Shammari, Abdullah IR - Al-Shammari A FIR - Choi, Jeesoo IR - Choi J FIR - Patel, Akshay IR - Patel A FIR - Bille, Andrea IR - Bille A FIR - Mimae, Takahiro IR - Mimae T FIR - Okada, Morihito IR - Okada M FIR - Karenovics, Wolfram IR - Karenovics W FIR - Bedat, Benoit IR - Bedat B FIR - Novoa, Nuria IR - Novoa N FIR - Casiraghi, Monica IR - Casiraghi M FIR - Raveglia, Federico IR - Raveglia F FIR - Cara, Andrea IR - Cara A FIR - Petrella, Francesco IR - Petrella F FIR - Hashimoto, Kohei IR - Hashimoto K FIR - Shibuya, Yukimi IR - Shibuya Y FIR - Tomaselli, Florian IR - Tomaselli F FIR - Monaci, Nicola IR - Monaci N FIR - Bouabdallah, Ilies IR - Bouabdallah I FIR - Streit, Arthur IR - Streit A FIR - Mendogni, Paolo IR - Mendogni P FIR - Nosotti, Mario IR - Nosotti M FIR - Cardillo, Giuseppe IR - Cardillo G FIR - Ricciardi, Sara IR - Ricciardi S FIR - Fang, Vincent IR - Fang V FIR - Luzzi, Luca IR - Luzzi L FIR - Catelli, Chiara IR - Catelli C FIR - Veres, Lukas IR - Veres L FIR - Desender, Liesbeth IR - Desender L FIR - Suzuki, Kei IR - Suzuki K EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/08/13 CRDT- 2026/10/28 05:11 PHST- 2026/04/09 00:00 [received] PHST- 2026/07/13 00:00 [revised] PHST- 2026/07/31 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:11 [entrez] PHST- 2026/08/13 00:00 [pmc-release] AID - S2666-2736(26)00476-6 [pii] AID - 102053 [pii] AID - 10.1016/j.xjon.2026.102053 [doi] PST - epublish SO - JTCVS Open. 2026 Aug 13;33:102053. doi: 10.1016/j.xjon.2026.102053. eCollection 2026 Oct. PMID- 42948084 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - A laboratory-based selected logistic model for early prediction of 28-day mortality in patients with septic shock. PG - 1864043 LID - 10.3389/fmed.2026.1864043 [doi] LID - 1864043 AB - BACKGROUND: Early risk stratification in septic shock requires transparent models based on variables available soon after clinical diagnosis of septic shock. We reanalysed a retrospective cohort using the original screening strategy: candidate predictors with univariable P < 0.05 were entered into multivariable logistic regression, and variables with multivariable P < 0.05 were retained in the final model. METHODS: The analysis included 250 patients with complete 28-day outcome and laboratory data. The outcome was 28-day mortality. Candidate predictors comprised demographics, comorbidities, complete blood count, chemistry, coagulation, inflammatory markers, arterial pH, and PaO2/FiO2. Laboratory values were treated as the first available values within 24 h after clinical diagnosis of septic shock. The final fixed model and the complete model-development pipeline were internally validated using 1,000 bootstrap resamples. Discrimination, calibration, Brier score, ROC-derived cutoff, decision curve analysis, LAR comparison, restricted cubic spline analysis, VIF, and LASSO sensitivity analysis were reported. RESULTS: Among 250 patients, 73 died by day 28 (29.2%). Univariable screening identified 12 candidate predictors, and multivariable screening retained lactate, D-dimer, and PaO2/FiO2. The final equation was logit(P) = 0.412145 + 0.250015 x Lactate + 0.046852 x D-dimer - 0.016820 x PaO2/FiO2. The apparent AUC was 0.902 (95% CI 0.864-0.940), and the optimism-corrected AUC for the fixed final model was 0.897. When the complete model-development pipeline was repeated within 1,000 bootstrap resamples, the optimism-corrected AUC was 0.875. The final model had higher discrimination than LAR alone [0.902 (0.864-0.940) vs. 0.719 (0.645-0.793); DeLong P < 0.001]. CONCLUSIONS: A selected logistic model using lactate, D-dimer, and PaO2/FiO2 provided internally validated early risk estimates for 28-day mortality. External validation is required before routine clinical use. CI - (c) 2026 Zhang, Chen, Li, Xiao and Zhou. FAU - Zhang, Liying AU - Zhang L AD - The Blood Transfusion Department, the First People's Hospital of Yibin City, Yibin, China. FAU - Chen, Pu AU - Chen P AD - The Laboratory Department, the Third People's Hospital of Yibin City, Yibin, China. FAU - Li, Jie AU - Li J AD - The Intensive Care Unit, the First People's Hospital of Yibin City, Yibin, China. FAU - Xiao, Weilei AU - Xiao W AD - The Laboratory Department, the First People's Hospital of Yibin City, Yibin, China. FAU - Zhou, Wentao AU - Zhou W AD - School of Laboratory Medicine, North Sichuan Medical College, Nanchong, China. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13710332 OTO - NOTNLM OT - D-dimer OT - PaO2/FiO2 OT - bootstrap validation OT - lactate OT - nomogram OT - prediction model OT - sepsis OT - septic shock COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:11 PHST- 2026/04/24 00:00 [received] PHST- 2026/09/07 00:00 [revised] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:11 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fmed.2026.1864043 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 13;13:1864043. doi: 10.3389/fmed.2026.1864043. eCollection 2026. PMID- 42948083 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2666-2736 (Electronic) IS - 2666-2736 (Linking) VI - 33 DP - 2026 Oct TI - Prognostic and clinicopathologic divergence in pulmonary mucinous adenocarcinoma versus nonmucinous adenocarcinoma with a mucin-laden feature. PG - 101964 LID - 10.1016/j.xjon.2026.101964 [doi] LID - 101964 AB - BACKGROUND: The discrepancies in clinicopathologic characteristics and prognosis between invasive mucinous adenocarcinoma (IMA) and nonmucinous lung adenocarcinoma with a mucin-laden feature (MNLA) remain unclear. METHODS: Data were collected retospectively for 978 consecutive patients with IMA/MNLA who underwent surgical resection between October 2017 and December 2019. Survival outcomes were estimated using the Kaplan-Meier method and compared using the log-rank test. The adjusted hazard ratio (aHR) with 95% confidence interval (CI) of the prognosticator was calculated using a multivariable Cox proportional hazards model. RESULTS: Pure IMA was associated with a higher prevalence of KRAS mutation (51.5%), while MNLA was associated with higher prevalences of EGFR mutation (40.4%) and EML4-ALK rearrangement (19.1%). Regarding prognosis, compared to patients with pure IMA, patients with MNLA or mixed IMA had worse disease-free survival (MNLA: aHR, 2.620, 95% CI, 1.690-4.061; mixed IMA: aHR, 3.458, 95% CI, 1.704-7.016) and overall survival (MNLA: aHR, 2.229, 95% CI, 1.185-4.193; mixed IMA: aHR, 1.723, 95% CI, 0.632-4.698). Among patients who experienced recurrence, those with pure IMA had a higher incidence of intrathoracic-only recurrence compared to those with MNLA (80.8% vs 39.6%), demonstrating that extrathoracic-only recurrence was more prevalent in MNLA (35.1% vs 7.3%). The recurrence pattern of mixed IMA was different from that of pure IMA (pure IMA vs mixed IMA: intrathoracic-only, 80.8% vs 50.0%; both intrathoracic and extrathoracic, 11.5% vs 40.0%). CONCLUSIONS: Although MNLA, pure IMA, and mixed IMA have consistent mucin-laden features, they appear to exhibit differences in prognosis, clinicopathologic characteristics, and molecular profiles. CI - (c) 2026 The Authors. FAU - Yang, Fujun AU - Yang F AD - Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University, School of Medicine, Shanghai, China. FAU - Sun, Ke AU - Sun K AD - Department of Radiology, Huashan Hospital, Fudan University, Shanghai, China. FAU - Li, Zhaoxun AU - Li Z AD - Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University, School of Medicine, Shanghai, China. FAU - You, Aijun AU - You A AD - Clinical Research Unit, Shanghai Tongji Hospital, School of Medicine, Tongji University, Shanghai, China. FAU - Yang, Zhen AU - Yang Z AD - Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University, School of Medicine, Shanghai, China. AD - Department of Thoracic Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. FAU - Wu, Yimu AU - Wu Y AD - Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University, School of Medicine, Shanghai, China. FAU - Li, Mengxing AU - Li M AD - Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University, School of Medicine, Shanghai, China. FAU - Liu, Yu AU - Liu Y AD - Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University, School of Medicine, Shanghai, China. FAU - Bian, Dongliang AU - Bian D AD - Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University, School of Medicine, Shanghai, China. FAU - Guo, Junhong AU - Guo J AD - Department of Pathology, Shanghai Pulmonary Hospital, Tongji University, School of Medicine, Shanghai, China. FAU - Jiang, Gening AU - Jiang G AD - Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University, School of Medicine, Shanghai, China. LA - eng PT - Journal Article DEP - 20260714 PL - Netherlands TA - JTCVS Open JT - JTCVS open JID - 101768541 PMC - PMC13711208 OTO - NOTNLM OT - clinicopathologic features OT - driver mutations OT - lung adenocarcinoma OT - molecular parameter OT - mucin-laden OT - prognosis OT - recurrence patterns COIS- The authors reported no conflicts of interest. The Journal policy requires editors and reviewers to disclose conflicts of interest and to decline handling or reviewing manuscripts for which they may have a conflict of interest. The editors and reviewers of this article have no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/07/14 CRDT- 2026/10/28 05:11 PHST- 2025/12/10 00:00 [received] PHST- 2026/05/23 00:00 [revised] PHST- 2026/07/07 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:11 [entrez] PHST- 2026/07/14 00:00 [pmc-release] AID - S2666-2736(26)00387-6 [pii] AID - 101964 [pii] AID - 10.1016/j.xjon.2026.101964 [doi] PST - epublish SO - JTCVS Open. 2026 Jul 14;33:101964. doi: 10.1016/j.xjon.2026.101964. eCollection 2026 Oct. PMID- 42948075 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 17 DP - 2026 TI - Toward mechanistic endotyping in allergic rhinitis: a State-Place-Time framework for appraising nasal tissue evidence. PG - 1972016 LID - 10.3389/fimmu.2026.1972016 [doi] LID - 1972016 AB - Allergic rhinitis (AR) heterogeneity is usually described by sensitization, symptom burden, or inflammatory profiles, but these descriptors do not establish which mechanism operates in a particular nasal mucosa. This critical narrative review proposes a State-Place-Time framework that evaluates cellular programs together with their anatomical setting and exposure-related response phase. The framework distinguishes descriptive tissue states from candidate mechanistic modules, measurable mechanistic endotypes, and clinically actionable endotypes. These are operational definitions for this review, rather than a validated classification. Five overlapping examples are examined: IgE-FcϵRI effector activity, late-phase type 2 amplification, barrier-repair dysfunction, separable sensory, vascular, and secretory outputs, and putative stromal maintenance. An author-derived conceptual matrix summarizes human evidence, spatial localization, timing/recurrence, matched perturbation, accessible measurement, and clinical utility; it does not provide an evidence score or patient assignment rule. Human intervention supports IgE-pathway dependence, but this does not establish a validated nasal tissue endotype for treatment selection. Late-phase cytokine responses and epithelial abnormalities are supported by human observations and functional systems, while their stability and predictive utility remain incompletely established. Stromal maintenance remains a hypothesis, with important limits from nasal polyposis sampling and inferred communication. The framework complements existing clinical and inflammatory classifications by identifying missing links and testable alternatives. Progress toward clinical use requires reproducibility within patients, analytical and external validation, and incremental value beyond routine assessment; treatment-selection claims additionally require evidence of differential treatment benefit. CI - Copyright (c) 2026 Meng and Gao. FAU - Meng, Ye AU - Meng Y AD - Department of Otolaryngology-Head and Neck Surgery, Binhai County People's Hospital, Yancheng, Jiangsu, China. FAU - Gao, Xiang AU - Gao X AD - Department of Otolaryngology-Head and Neck Surgery, Binhai County People's Hospital, Yancheng, Jiangsu, China. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 37341-29-0 (Immunoglobulin E) SB - IM MH - Humans MH - *Nasal Mucosa/immunology/pathology/metabolism MH - *Rhinitis, Allergic/immunology/diagnosis MH - Animals MH - Immunoglobulin E/immunology/metabolism PMC - PMC13711116 OTO - NOTNLM OT - allergic rhinitis OT - cellular heterogeneity OT - mechanistic endotype OT - mucosal immunity OT - nasal mucosa OT - spatial organization OT - temporal dynamics OT - tissue state COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:10 PHST- 2026/08/19 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:10 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fimmu.2026.1972016 [doi] PST - epublish SO - Front Immunol. 2026 Oct 13;17:1972016. doi: 10.3389/fimmu.2026.1972016. eCollection 2026. PMID- 42948072 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - KAT6B::KANSL1 fusion-positive uterine mesenchymal neoplasm with hybrid endometrial stromal and smooth muscle features: a case report. PG - 1859849 LID - 10.3389/fmed.2026.1859849 [doi] LID - 1859849 AB - KAT6B/A::KANSL1 fusion-positive uterine mesenchymal neoplasms are rare and may show overlapping endometrial stromal and smooth muscle features. We report a 47-year-old woman with regular menses, no dysmenorrhea, and a 7-year history of a presumed uterine fibroid. She underwent laparoscopic myomectomy at an outside hospital in November 2024. The fragmented specimen was interpreted as an endometrial stromal tumor with smooth muscle differentiation, but the tumor-myometrium interface could not be assessed, and an endometrial stromal nodule could not be distinguished from low-grade endometrial stromal sarcoma. Subsequent pelvic magnetic resonance imaging showed postoperative changes, possible residual leiomyomas, and heterogeneous enhancement at the uterine fundus. Pelvic computed tomography showed heterogeneous uterine enhancement without definite extrauterine disease. Because the original specimen was fragmented and a potentially malignant uterine mesenchymal neoplasm could not be excluded, completion hysterectomy with bilateral salpingo-oophorectomy was performed. The hysterectomy specimen contained multiple residual intramyometrial nodules with marked regional heterogeneity. One component showed fascicular smooth muscle morphology and diffuse desmin and h-caldesmon expression, whereas other nodules showed tongue-like myometrial infiltration, focal intravascular growth, spiral arteriole-like vessels, and diffuse ER, PR, CD10, and SMA expression. Whole-transcriptome sequencing identified an in-frame KAT6B::KANSL1 fusion involving KAT6B exon 3 and KANSL1 exon 11. Because the tumor expressed ER and PR, postoperative letrozole was administered for 30 days, after which surveillance was selected following review of the final pathologic and molecular findings. At 8 months after completion surgery, no clinical or radiologic recurrence was detected; this limited follow-up does not permit conclusions regarding long-term biological behavior or endocrine treatment benefit. CI - (c) 2026 Li and Wu. FAU - Li, Qin AU - Li Q AD - The Second Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou, China. FAU - Wu, Meijuan AU - Wu M AD - Zhejiang Cancer Hospital, Hangzhou, China. LA - eng PT - Case Reports PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13710359 OTO - NOTNLM OT - KAT6B::KANSL1 fusion OT - endometrial stromal sarcoma OT - molecular diagnostics OT - smooth muscle neoplasm OT - uterine sarcoma COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:10 PHST- 2026/04/19 00:00 [received] PHST- 2026/08/30 00:00 [revised] PHST- 2026/09/14 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:10 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fmed.2026.1859849 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 13;13:1859849. doi: 10.3389/fmed.2026.1859849. eCollection 2026. PMID- 42948056 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Associations between corneal biomechanical parameters and retinal superficial vessel density in young adults with varying myopia severity: a cross-sectional study. PG - 1951319 LID - 10.3389/fmed.2026.1951319 [doi] LID - 1951319 AB - AIM: To evaluate associations between corneal biomechanical parameters and superficial retinal vessel density (SVD) in young adults with varying myopia severity and determine whether these associations differ myopia severity groups. METHODS: This cross-sectional study included 167 participants contributing 320 eyes (93 low-, 125 moderate-, and 102 high-myopia eyes). Corneal biomechanical parameters were assessed using Corneal Visualization Scheimpflug Technology (Corvis ST), and macular and peripapillary SVD using optical coherence tomography angiography (OCTA). Eye-level analyses used linear mixed-effects models (LMMs) with participant identity as a random intercept to account for inter-eye correlation. Association models were adjusted for axial length, age, sex, and biomechanically corrected intraocular pressure (bIOP). Biomechanical parameter x myopia severity interactions were tested to assess between-group differences in associations. RESULTS: Peak distance (PD), stress-strain index (SSI), stiffness parameter at first applanation (SP-A1), and Ambrosio relational thickness-horizontal (ARTh) differed significantly among myopia groups (all P < 0.05). Macular SVD differed in the inner nasal (IN) and outer nasal (ON) regions, while peripapillary SVD differed in the IN, inner temporal (IT), outer superior (OS), outer inferior (OI), ON, and outer temporal (OT) regions (all P < 0.05). After covariate adjustment, several corneal biomechanical parameters remained associated with regional SVD. IRA x myopia severity interactions were significant for central, II, IN, IT, and OT macular SVD (all P for interaction < 0.05), while PD also showed a significant interaction with myopia severity for central SVD (P for interaction = 0.023). For peripapillary SVD, a significant IRA x myopia severity interaction was observed in the OS region (P for interaction = 0.015). CONCLUSION: Corneal biomechanical parameters and retinal SVD showed regional differences across myopia severity groups. After accounting for inter-eye correlation and relevant covariates, selected biomechanical parameters were associated with regional SVD, and some associations differed by myopia severity. These findings suggest a potential relationship between anterior segment biomechanical characteristics and retinal microvascular alterations; however, the cross-sectional design precludes causal or longitudinal interpretation. CI - (c) 2026 Guo, Liu, Wang, Zhang, He, Zhang, Chen, Chen, Zhong and Liao. FAU - Guo, Yanzhi AU - Guo Y AD - Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai, China. FAU - Liu, Xiaohong AU - Liu X AD - Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai, China. FAU - Wang, Yiwei AU - Wang Y AD - Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai, China. FAU - Zhang, Yang AU - Zhang Y AD - Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai, China. FAU - He, Siqi AU - He S AD - Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai, China. FAU - Zhang, Yumeng AU - Zhang Y AD - Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai, China. FAU - Chen, Junjue AU - Chen J AD - Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai, China. FAU - Chen, Jili AU - Chen J AD - Department of Ophthalmology, Shibei Hospital, Shanghai, China. FAU - Zhong, Yisheng AU - Zhong Y AD - Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai, China. FAU - Liao, Huaping AU - Liao H AD - Department of Ophthalmology, Ruijin Hospital Affiliated Medical School, Shanghai Jiaotong University, Shanghai, China. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13710290 OTO - NOTNLM OT - Corvis ST OT - OCTA OT - association OT - corneal biomechanical properties OT - myopia COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:09 PHST- 2026/07/28 00:00 [received] PHST- 2026/09/13 00:00 [revised] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:09 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fmed.2026.1951319 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 13;13:1951319. doi: 10.3389/fmed.2026.1951319. eCollection 2026. PMID- 42948043 OWN - NLM STAT- Publisher LR - 20261029 IS - 2635-098X (Electronic) IS - 2635-098X (Linking) DP - 2026 Oct 16 TI - Sampling a trillion-sized GDB-20 database of drug-like molecules by generative artificial intelligence. LID - 10.1039/d6dd00213g [doi] AB - Identifying new chemical entities for small molecule drug discovery requires the continued exploration of its vast chemical space, which has been speculated to contain 10(60) or more molecules. Here we used generative artificial intelligence to sample a GDB-20 database estimated to contain 32 trillion organic molecules corresponding to 26.9 million molecular graphs up to 20 nodes selected for their limited topological complexity. We show that GDB-20 populates a distinct chemical space of sp(3)-rich, 3D-shaped and natural product-like molecules with favorable synthetic accessibility score. The relevance for drug discovery of our 12 billion sample, named GDB-20s and corresponding in size to approximately 0.04% of the estimated full database, is suggested by high compliance with drug-likeness and toxicity filters, and the coverage of thousands of scaffolds occurring in bioactive molecules recorded in the ChEMBL database. The generative models used to produce GDB-20s, together with the 12-billion-molecule collection are available for download at https://gdb.unibe.ch. CI - This journal is (c) The Royal Society of Chemistry. FAU - Buehler, Ye AU - Buehler Y AUID- ORCID: 0000-0002-8139-830X AD - Department of Chemistry, Biochemistry and Pharmaceutical Sciences, University of Bern Bern 3012 Switzerland jean-louis.reymond@unibe.ch. FAU - Javor, Sacha AU - Javor S AD - Department of Chemistry, Biochemistry and Pharmaceutical Sciences, University of Bern Bern 3012 Switzerland jean-louis.reymond@unibe.ch. FAU - Reymond, Jean-Louis AU - Reymond JL AUID- ORCID: 0000-0003-2724-2942 AD - Department of Chemistry, Biochemistry and Pharmaceutical Sciences, University of Bern Bern 3012 Switzerland jean-louis.reymond@unibe.ch. LA - eng PT - Journal Article DEP - 20261016 PL - England TA - Digit Discov JT - Digital discovery JID - 9918351186906676 PMC - PMC13711157 COIS- There are no conflicts to declare. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/16 CRDT- 2026/10/28 05:09 PHST- 2026/04/21 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:09 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - d6dd00213g [pii] AID - 10.1039/d6dd00213g [doi] PST - aheadofprint SO - Digit Discov. 2026 Oct 16. doi: 10.1039/d6dd00213g. PMID- 42948033 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2234-943X (Print) IS - 2234-943X (Electronic) IS - 2234-943X (Linking) VI - 16 DP - 2026 TI - Patient selection strategies for deep inspiration breath-hold in left-sided breast cancer: a review of current evidence and future directions. PG - 1949600 LID - 10.3389/fonc.2026.1949600 [doi] LID - 1949600 AB - With accumulating evidence confirming that postoperative adjuvant radiotherapy improves survival outcomes in patients with left-sided breast cancer, the long-term cardiotoxicity risks associated with radiotherapy have attracted widespread attention. The deep inspiration breath hold (DIBH) technique increases the distance between the heart and the breast target, effectively reducing radiation dose to the heart and coronary arteries. However, the resource-intensive nature of DIBH and substantial heterogeneity in therapeutic benefits among patients pose a major clinical challenge: how to accurately and efficiently identify patients suitable for DIBH. This narrative review summarizes current evidence regarding DIBH patient selection, and integrates available strategies with clinical workflows to construct a three-tiered decision-making framework encompassing feasibility assessment, indication evaluation, and clinical benefit assessment. Given the existing gaps in high-level prospective clinical evidence, three primary research directions are proposed: (1) conducting large-scale multi-center prospective cohort studies to validate the generalizability and clinical practicality of selection strategies; (2) developing an individualized normal tissue complication probability (NTCP) model integrating multi-dimensional risk factors to guide precise decision-making; and (3) implementing prospective clinical trials of artificial intelligence (AI)-assisted selection platforms to accelerate the clinical translation of intelligent selection tools. This review synthesizes current evidence on DIBH patient selection, constructs a hierarchical decision framework to provide a reference for clinical practice, and delineates directions for future research. CI - Copyright (c) 2026 Sun, Huang, Zhou, Zhou, Wang, Luo, Zhou, Zeng and Ni. FAU - Sun, Xiangshang AU - Sun X AD - Department of Radiation Oncology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University/Hunan Cancer Hospital, Changsha, China. FAU - Huang, Shixiong AU - Huang S AD - Department of Radiation Oncology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University/Hunan Cancer Hospital, Changsha, China. FAU - Zhou, Xing AU - Zhou X AD - Department of Radiation Oncology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University/Hunan Cancer Hospital, Changsha, China. FAU - Zhou, Borong AU - Zhou B AD - Department of Radiation Oncology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University/Hunan Cancer Hospital, Changsha, China. FAU - Wang, Zhiyan AU - Wang Z AD - Department of Radiation Oncology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University/Hunan Cancer Hospital, Changsha, China. FAU - Luo, Lingrong AU - Luo L AD - Department of Chest Radiation Oncology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University/Hunan Cancer Hospital, Changsha, China. FAU - Zhou, Qionghui AU - Zhou Q AD - Department of Radiation Oncology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University/Hunan Cancer Hospital, Changsha, China. FAU - Zeng, Biao AU - Zeng B AD - Department of Radiation Oncology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University/Hunan Cancer Hospital, Changsha, China. FAU - Ni, Qianxi AU - Ni Q AD - Department of Radiation Oncology, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University/Hunan Cancer Hospital, Changsha, China. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Oncol JT - Frontiers in oncology JID - 101568867 PMC - PMC13711051 OTO - NOTNLM OT - artificial intelligence OT - breast cancer OT - cardiac sparing OT - deep inspiration breath-hold OT - patient selection COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:08 PHST- 2026/07/27 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:08 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fonc.2026.1949600 [doi] PST - epublish SO - Front Oncol. 2026 Oct 13;16:1949600. doi: 10.3389/fonc.2026.1949600. eCollection 2026. PMID- 42948032 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-875X (Print) IS - 2296-875X (Electronic) IS - 2296-875X (Linking) VI - 13 DP - 2026 TI - Case Report: Talonavicular joint replacement in congenital talonavicular coalition with individualized 3D-printed prosthesis. PG - 1865047 LID - 10.3389/fsurg.2026.1865047 [doi] LID - 1865047 AB - BACKGROUND: Talonavicular coalition is a rare type of tarsal coalition that may lead to painful flatfoot and progressive functional impairment. Conventional talonavicular arthrodesis provides symptom relief but sacrifices joint motion. Motion-preserving reconstruction strategies remain largely unexplored. CASE PRESENTATION: We report a case of congenital talonavicular coalition accompanied by flatfoot treated with individualized 3D-printed talonavicular joint replacement (TNJR) in a 12-year-old patient. At 24-month follow-up, the patient achieved complete pain relief (VAS 0) and significant functional improvement (AOFAS 98). Talonavicular joint motion was preserved with 15 degrees inversion/eversion and 15 degrees pronation/supination. Radiographic evaluation demonstrated restoration of the medial longitudinal arch and correction of hindfoot valgus. Progressive improvement was observed in the calcaneal pitch, talonavicular coverage, and hindfoot alignment angles, whereas Meary's angle showed a slight increase. CONCLUSION: This case demonstrates the feasibility of individualized 3D-printed TNJR as a potential motion-preserving option for selected patients with extensive congenital talonavicular coalition. CI - (c) 2026 Li, Liu, Yuan, Zhao, Zhou, Liu, Liu, Ji, Liu and Sun. FAU - Li, Guimiao AU - Li G AD - Department of Orthopedics, The First Hospital of Hebei Medical University, Shijiazhuang, China. FAU - Liu, Shixin AU - Liu S AD - Department of Foot and Ankle Surgery, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei, China. FAU - Yuan, Chenchen AU - Yuan C AD - Hebei Medical University, Shijiazhuang, China. FAU - Zhao, Leilei AU - Zhao L AD - Department of Orthopedics, The First Hospital of Hebei Medical University, Shijiazhuang, China. FAU - Zhou, Hecheng AU - Zhou H AD - Department of Orthopedics, The First Hospital of Hebei Medical University, Shijiazhuang, China. FAU - Liu, Dehua AU - Liu D AD - Department of Orthopedics, The First Hospital of Hebei Medical University, Shijiazhuang, China. FAU - Liu, Dehang AU - Liu D AD - Department of Orthopedics, The First Hospital of Hebei Medical University, Shijiazhuang, China. FAU - Ji, Gang AU - Ji G AD - Department of Orthopedics, The First Hospital of Hebei Medical University, Shijiazhuang, China. FAU - Liu, Guobin AU - Liu G AD - Department of Orthopedics, The First Hospital of Hebei Medical University, Shijiazhuang, China. FAU - Sun, Zhenhui AU - Sun Z AD - Department of Foot and Ankle Surgery, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei, China. LA - eng PT - Case Reports PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Surg JT - Frontiers in surgery JID - 101645127 PMC - PMC13710302 OTO - NOTNLM OT - 3D-printed prosthesis OT - flatfoot OT - motion preservation surgery OT - talonavicular coalition OT - talonavicular joint replacement COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:08 PHST- 2026/04/25 00:00 [received] PHST- 2026/08/20 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:08 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fsurg.2026.1865047 [doi] PST - epublish SO - Front Surg. 2026 Oct 13;13:1865047. doi: 10.3389/fsurg.2026.1865047. eCollection 2026. PMID- 42948027 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2198-7793 (Print) IS - 2198-7793 (Electronic) IS - 2198-7793 (Linking) VI - 12 IP - 1 DP - 2026 TI - Long-Term Survival after Complete Resection of Local Duodenal Recurrence following Colon Cancer Surgery Using Laparoscopic and Endoscopic Cooperation: A Case Report. LID - 10.70352/scrj.cr.26-0565 [doi] LID - 26-0565 AB - INTRODUCTION: Laparoscopic and endoscopic cooperative surgery (LECS) enables accurate intraluminal localization and limited full-thickness resection while preserving gastrointestinal continuity. Although LECS has been used for selected primary duodenal tumors, its application to a postoperative duodenal recurrence of colorectal cancer has rarely been described. We report a case of localized duodenal recurrence after a right hemicolectomy that was completely resected using laparoscopic and endoscopic cooperation. CASE PRESENTATION: An 83-year-old man underwent a laparoscopic right hemicolectomy with a D3 lymph node dissection for advanced ascending colon cancer. Histopathological examination demonstrated a microscopically positive dissected margin on the duodenal side, indicating a microscopically positive margin (R1) resection. Nine months later, surveillance upper gastrointestinal endoscopy and contrast-enhanced CT revealed a solitary lesion in the second portion of the duodenum without distant metastasis or radiological pancreatic involvement. Endoscopic ultrasonography showed a lesion extending to the fourth layer. An upper gastrointestinal contrast study was not performed; instead, the location and local extent of the lesion were assessed by upper gastrointestinal endoscopy, endoscopic ultrasonography, and contrast-enhanced CT. Because the multimodal assessment indicated that complete local excision was technically achievable, LECS was performed. Intraoperative endoscopy delineated a wider intraluminal extent than was apparent from the serosal surface and guided an en bloc full-thickness resection. The defect was closed in the short-axis direction by a continuous full-thickness suturing. Intraoperative endoscopy confirmed the luminal patency and a negative leak test. Histopathological examination showed an adenocarcinoma involving the submucosa and muscularis propria without duodenal mucosal involvement. The adjacent muscularis propria was inverted toward the lumen, suggesting inward progression from the outer aspect of the duodenal wall. Together with the previous R1, these findings supported a localized recurrence from microscopic residual disease. The resection margins were negative, and the patient has remained disease-free for more than 5 years. CONCLUSIONS: Laparoscopic and endoscopic cooperation enabled precise definition of the recurrent lesion, complete full-thickness local resection, and secure closure while preserving duodenal continuity. This approach may be useful for strictly localized duodenal recurrence when careful preoperative assessment indicates that complete local excision is achievable. CI - (c) 2026 The Author(s). Published by Japan Surgical Society. FAU - Kameda, Chizu AU - Kameda C AD - Department of Surgery, Japanese Red Cross Fukuoka Hospital, Fukuoka, Fukuoka, Japan. AD - Department of Surgery, Kyushu Railway Memorial Hospital, Kitakyushu, Fukuoka, Japan. FAU - Oda, Yasunori AU - Oda Y AD - Department of Surgery, Japanese Red Cross Fukuoka Hospital, Fukuoka, Fukuoka, Japan. AD - Department of Surgery, Kyushu Railway Memorial Hospital, Kitakyushu, Fukuoka, Japan. FAU - Takahata, Shunichi AU - Takahata S AD - Department of Surgery, Kyushu Railway Memorial Hospital, Kitakyushu, Fukuoka, Japan. FAU - Nagai, Eishi AU - Nagai E AD - Department of Surgery, Japanese Red Cross Fukuoka Hospital, Fukuoka, Fukuoka, Japan. FAU - Nakafusa, Yuji AU - Nakafusa Y AD - Department of Surgery, Japanese Red Cross Fukuoka Hospital, Fukuoka, Fukuoka, Japan. LA - eng PT - Case Reports PT - Journal Article DEP - 20261024 PL - Japan TA - Surg Case Rep JT - Surgical case reports JID - 101662125 PMC - PMC13711107 OTO - NOTNLM OT - colorectal cancer OT - duodenal local recurrence OT - laparoscopic and endoscopic cooperative surgery OT - local resection OT - long-term survival COIS- The authors declare that they have no competing interests. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/24 CRDT- 2026/10/28 05:08 PHST- 2026/07/23 00:00 [received] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:08 [entrez] PHST- 2026/10/24 00:00 [pmc-release] AID - scrj.cr.26-0565 [pii] AID - 10.70352/scrj.cr.26-0565 [doi] PST - ppublish SO - Surg Case Rep. 2026;12(1):26-0565. doi: 10.70352/scrj.cr.26-0565. Epub 2026 Oct 24. PMID- 42948023 OWN - NLM STAT- Publisher LR - 20261029 IS - 2475-0328 (Electronic) IS - 2475-0328 (Linking) DP - 2026 Oct 27 TI - Metastasis Pattern-Specific Biomarkers and Therapeutic Targets in Gastric Cancer: From Surgical Specimens to Clinical Translation. LID - 10.1002/ags3.70302 [doi] AB - Metastasis has traditionally been investigated and treated as a single manifestation of gastric cancer progression. Although this approach has generated important biological insights and effective systemic therapies, it may obscure route-dependent vulnerabilities. Lymphatic, peritoneal, and hematogenous metastases require cancer cells to traverse different anatomical routes, overcome distinct barriers, and adapt to different microenvironments. This review therefore distinguishes broadly metastasis-associated mechanisms from pattern-associated candidates and reserves pattern-specific designation for evidence based on direct route comparison, pattern prediction, or route-selective functional experiments. We examine representative molecular determinants and link their identification in surgical specimens to functional validation, biomarker development, and drug discovery. Evidence is most mature for peritoneal dissemination, whereas lymph-node-specific determinants remain less developed. The SYT13/ASO-4733 program is presented as an illustrative translational case: Intraperitoneal ASO-4733 has entered first-in-human Phase I evaluation, but clinical efficacy has not been established. For any candidate, clinical utility requires independent validation, incremental value beyond established clinicopathological factors, and a defined effect on decision-making. Rigorously collected, rapidly processed, and clinically annotated surgical biospecimens remain indispensable. Reframing gastric cancer metastasis by route may expand opportunities for precise risk prediction, rational delivery, and biologically coherent early-phase trials. CI - (c) 2026 The Author(s). Annals of Gastroenterological Surgery published by John Wiley & Sons Australia, Ltd on behalf of The Japanese Society of Gastroenterological Surgery. FAU - Kanda, Mitsuro AU - Kanda M AUID- ORCID: 0000-0001-5464-3819 AD - Department of Gastroenterological Surgery Nagoya University Graduate School of Medicine Nagoya Japan. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Japan TA - Ann Gastroenterol Surg JT - Annals of gastroenterological surgery JID - 101718062 PMC - PMC13711042 OTO - NOTNLM OT - biomarkers OT - gastric cancer OT - metastasis pattern OT - surgical biospecimens OT - therapeutic targets COIS- The author declares no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:36 PMCR- 2026/10/27 CRDT- 2026/10/28 05:08 PHST- 2026/09/28 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 11:36 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:08 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - AGS370302 [pii] AID - 10.1002/ags3.70302 [doi] PST - aheadofprint SO - Ann Gastroenterol Surg. 2026 Oct 27:10.1002/ags3.70302. doi: 10.1002/ags3.70302. PMID- 42948019 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2042-8812 (Print) IS - 2042-8812 (Electronic) IS - 2042-8812 (Linking) VI - 2026 IP - 10 DP - 2026 Oct TI - Neurological deterioration after posterior decompression of an unrecognised calcified thoracic disc: recovery following endoscopic discectomy. PG - rjag970 LID - 10.1093/jscr/rjag970 [doi] LID - rjag970 AB - Calcified thoracic disc herniation is uncommon but carries substantial operative risk. A 67-year-old man with 12 months of progressive myelopathy from a T7-T8 disc herniation underwent posterior laminectomy and instrumented fusion without preoperative computed tomography (CT). He developed near-complete paraplegia, with lower-limb power of Medical Research Council grade 0-1/5. Subsequent CT demonstrated a densely calcified ventral disc. Following transfer, full-endoscopic transforaminal discectomy was performed to address the ventral compression without spinal cord retraction. By three months after the revision procedure, power improved to grade 4-4+/5 and he regained ambulation using a walking frame. Thoracic disc herniations being considered for surgery should be characterised with CT as well as magnetic resonance imaging. Calcified lesions warrant subspecialty review and an approach that directly addresses the ventral pathology. In selected patients, endoscopic discectomy may achieve effective decompression with limited access morbidity. CI - (c) The Author(s) 2026. Published by Oxford University Press and JSCR Publishing Ltd. FAU - Mobbs, Ralph J AU - Mobbs RJ AD - Faculty of Medicine and Health, University of New South Wales, High St, Kensington, NSW 2052, Australia. AD - NeuroSpine Clinic, Prince of Wales Private Hospital, Barker St, Randwick, NSW 2031, Australia. AD - NeuroSpine Surgery Research Group (NSURG), Barker St, Randwick, NSW 2031, Australia. AD - Department of Neurosurgery, Prince of Wales Private Hospital, Barker St, Randwick, NSW 2031, Australia. FAU - Lui, Edwin H Y AU - Lui EHY AUID- ORCID: 0009-0000-5266-4253 AD - Faculty of Medicine and Health, University of New South Wales, High St, Kensington, NSW 2052, Australia. AD - NeuroSpine Clinic, Prince of Wales Private Hospital, Barker St, Randwick, NSW 2031, Australia. AD - NeuroSpine Surgery Research Group (NSURG), Barker St, Randwick, NSW 2031, Australia. AD - Department of Neurosurgery, Prince of Wales Private Hospital, Barker St, Randwick, NSW 2031, Australia. FAU - Reidy, Joseph AU - Reidy J AD - NeuroSpine Surgery Research Group (NSURG), Barker St, Randwick, NSW 2031, Australia. AD - Department of Neurosurgery, Prince of Wales Hospital, Barker St, Randwick, NSW 2031, Australia. LA - eng PT - Case Reports PT - Journal Article DEP - 20261027 PL - England TA - J Surg Case Rep JT - Journal of surgical case reports JID - 101560169 PMC - PMC13711075 OTO - NOTNLM OT - calcified thoracic disc OT - endoscopic discectomy OT - laminectomy OT - neurological deterioration OT - thoracic myelopathy COIS- R.J.M. is a consultant to Matrix Medical. E.L. and J.R. declare no competing interests. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/27 CRDT- 2026/10/28 05:07 PHST- 2026/07/20 00:00 [received] PHST- 2026/09/07 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:07 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - rjag970 [pii] AID - 10.1093/jscr/rjag970 [doi] PST - epublish SO - J Surg Case Rep. 2026 Oct 27;2026(10):rjag970. doi: 10.1093/jscr/rjag970. eCollection 2026 Oct. PMID- 42948009 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2674-0850 (Electronic) IS - 2674-0850 (Linking) VI - 6 DP - 2026 TI - Metal-organic and covalent organic frameworks for precision drug delivery: a precision-by-design evidence framework for biologically accountable nanomedicine. PG - 1970554 LID - 10.3389/fddev.2026.1970554 [doi] LID - 1970554 AB - INTRODUCTION: Metal-organic frameworks and covalent organic frameworks offer a rare opportunity to design drug carriers from molecular building blocks upward. Yet high loading capacity and stimulus-responsive release alone do not constitute precision delivery. METHODS: We critically synthesized evidence on MOF and COF pore-level cargo interactions, framework and surface engineering, endogenous and externally triggered release, active targeting, biomimetic coatings, nanozyme activity and theranostic integration. Precision delivery was decomposed into five evidence-qualified dimensions: material, spatial, temporal, mechanistic and translational precision. RESULTS: The evidence supports the complementary strengths of the two families across small-molecule, protein, nucleic-acid, gas and catalytic therapies. Direct evidence is strongest for individual component technologies, selected antibody-targeted systems and two-component hybrids. Recurrent evidence gaps include incomplete particle characterization in biological media, reliance on total-tissue accumulation rather than cell-specific delivery, and insufficient separation of carrier degradation from drug release. DISCUSSION: A framework becomes precise only when it maintains a defined material identity, reaches a biologically justified compartment, releases an active payload at the intended place and time, and does so with measurable improvements in exposure, efficacy and safety. This requires a precision-by-design framework that maps claims to minimum evidence and links material critical quality attributes to biological mechanisms, translational assays and development decisions. CI - Copyright (c) 2026 Li, Zhang, Ding and Wang. FAU - Li, Xinwei AU - Li X AD - Organ Transplantation Center, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Zhang, Haonan AU - Zhang H AD - Organ Transplantation Center, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China. AD - University of Chinese Academy of Sciences, Beijing, China. FAU - Ding, Ruidong AU - Ding R AD - Organ Transplantation Center, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China. FAU - Wang, Feng AU - Wang F AD - Organ Transplantation Center, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Drug Deliv JT - Frontiers in drug delivery JID - 9918469589006676 PMC - PMC13710936 OTO - NOTNLM OT - biomimetic nanomedicine OT - covalent organic frameworks OT - metal-organic frameworks OT - nanozyme OT - precision drug delivery OT - precision-by-design OT - stimuli-responsive release OT - theranostics COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:06 PHST- 2026/08/17 00:00 [received] PHST- 2026/09/07 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:06 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 1970554 [pii] AID - 10.3389/fddev.2026.1970554 [doi] PST - epublish SO - Front Drug Deliv. 2026 Oct 13;6:1970554. doi: 10.3389/fddev.2026.1970554. eCollection 2026. PMID- 42948008 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-2565 (Electronic) IS - 2296-2565 (Linking) VI - 14 DP - 2026 TI - Clinical factors associated with septic shock in pediatric invasive meningococcal disease: a retrospective study from two tertiary hospitals in Chile, 2009-2025. PG - 1911106 LID - 10.3389/fpubh.2026.1911106 [doi] LID - 1911106 AB - BACKGROUND: Invasive meningococcal disease (IMD) remains a primary etiology of bacterial meningitis and a cause of long-term neurological sequelae in children. We aimed to describe characteristics of IMD and to identify variables associated with adverse outcomes, defined as septic shock. METHODS: A retrospective cohort study included children with microbiologically confirmed IMD hospitalized at two tertiary pediatric hospitals in Santiago, Chile (2009-2025). Data were collected from medical records. Variables associated with the outcome were assessed using Firth's penalized logistic regression, with candidate variables selected a priori according to clinical relevance, availability at admission, and absence of overlapping with the outcome process. RESULTS: Seventy-two children were included; 65.3% were male and 54.2% were infants younger than 12 months. Over the 17-year study period, most cases occurred between 2009 and 2014 (n = 51), followed by a marked decline from 2015 to 2025 (n = 21) coinciding with the introduction of the meningococcal ACWY vaccine in the National Immunization Program. The most frequent manifestations at admission were toxic appearance (79.2%), fever (69.4%), gastrointestinal symptoms (69.4%), and irritability or drowsiness (62.5%). The main diagnoses were meningitis with meningococcemia (37.5%), isolated meningitis (25.0%), and meningococcemia (25.0%). Serogroup W (50.0%) and serogroup B (36.1%) predominated. Septic shock occurred in 28 children (38.9%), with a case-fatality rate of 6.9% (5/72); all deaths occurred in children with meningococcemia and none in isolated meningitis. In the primary multivariable model, seizure during hospitalization (aOR 10.63; 2.33-63.90), prolonged capillary refill (aOR 5.75; 1.49-26.21), and petechial or purpuric rash (aOR 4.47; 1.24-18.87), were associated with adverse outcome. CONCLUSION: Pediatric IMD remains an important cause of severe disease and mortality. The factors associated with severe outcome reflected three clinically relevant domains: neurological involvement, impaired peripheral perfusion, and vascular manifestations. Because these are simple clinical signs recognizable without laboratory testing, they may support early risk stratification and timely intervention, particularly in resource-limited settings. CI - Copyright (c) 2026 Arteta-Acosta, Marquez, Villena and Santolaya. FAU - Arteta-Acosta, Cindy AU - Arteta-Acosta C AD - CICA Hospital de ninos Dr. Luis Calvo Mackenna, Department of Pediatrics, Faculty of Medicine, Universidad de Chile, Santiago, Chile. FAU - Marquez, Maritza AU - Marquez M AD - Pontificia Universidad Catolica de Chile, Faculty of Mathematics, Universidad Catolica de Chile, Santiago, Chile. FAU - Villena, Rodolfo AU - Villena R AD - Department of Pediatrics, CICA Hospital de ninos Dr. Exequiel Gonzalez Cortes, Faculty of Medicine, Universidad de Chile, Santiago, Chile. FAU - Santolaya, Maria Elena AU - Santolaya ME AD - CICA Hospital de ninos Dr. Luis Calvo Mackenna, Department of Pediatrics, Faculty of Medicine, Universidad de Chile, Santiago, Chile. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Public Health JT - Frontiers in public health JID - 101616579 SB - IM MH - Humans MH - Male MH - Retrospective Studies MH - *Shock, Septic/epidemiology MH - Chile/epidemiology MH - Female MH - Infant MH - Tertiary Care Centers MH - Child, Preschool MH - *Meningococcal Infections/epidemiology/complications MH - Risk Factors MH - Child PMC - PMC13710219 OTO - NOTNLM OT - meningitis OT - meningococcal infections OT - mortality OT - pediatrics OT - septic shock COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:06 PHST- 2026/06/16 00:00 [received] PHST- 2026/09/08 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:06 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fpubh.2026.1911106 [doi] PST - epublish SO - Front Public Health. 2026 Oct 13;14:1911106. doi: 10.3389/fpubh.2026.1911106. eCollection 2026. PMID- 42947996 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-042X (Print) IS - 1664-042X (Electronic) IS - 1664-042X (Linking) VI - 17 DP - 2026 TI - Relationships among heart rate variability, sleep disturbances, and fatigue in patients undergoing interventional therapy for hepatocellular carcinoma. PG - 1923785 LID - 10.3389/fphys.2026.1923785 [doi] LID - 1923785 AB - BACKGROUND: Fatigue and sleep disturbances are commonly experienced by patients with hepatocellular carcinoma (HCC). However, the potential association between these conditions and autonomic nervous system reflected by heart rate variability (HRV) is unclear. We aimed to evaluate the differences in HRV parameters among HCC patients with different degrees of fatigue and sleep disturbances, as well as the relationship between HRV parameters and fatigue and sleep disturbances. METHODS: In this cross-sectional study, a total of 66 patients with HCC hospitalized for interventional therapy were enrolled. Patients completed questionnaires during hospitalization, including the Pittsburgh Sleep Quality Index (PSQI) and the Fatigue Scale-14 (FS-14), and underwent a 5-minute resting electrocardiogram (ECG) for HRV analysis. Group comparisons and logistic regression models adjusted for age were used to examine the associations between HRV parameters and symptom severity, with Benjamini-Hochberg false discovery rate (FDR) correction for multiple comparisons. RESULTS: Acceleration capacity (AC) was significantly higher and low frequency (LF) was significantly lower in the high PSQI group compared to the low PSQI group (AC: p = 0.036; LF: p = 0.041); Compared to the low FS-14 group, the high FS-14 group had significantly higher mean heart rate (MeanHR) and AC, while most other HRV indices were significantly lower (MeanHR: p = 0.029; AC: p = 0.009). The results of the adjusted logistic regression model indicated that increased AC showed a marginal trend toward an association with poor sleep quality (p = 0.071), while MeanHR remained significantly associated with severe fatigue (OR = 1.098, p = 0.004). After FDR correction, the differences in eight HRV parameters between fatigue groups remained significant (all q < 0.05), whereas the differences between sleep-quality groups did not survive correction. CONCLUSIONS: Our findings suggest that alterations in HRV parameters, particularly mean heart rate (MeanHR) and acceleration capacity (AC), are associated with the severity of fatigue and sleep disturbances in HCC patients. Given the exploratory cross-sectional design, these metrics should be regarded as candidate non-invasive physiological correlates of autonomic dysfunction and symptom burden. Their clinical relevance requires confirmation in larger prospective studies. CI - Copyright (c) 2026 Xie, Yun, Xu, Meng, Peng, Xie, Zhou, Xu and Xu. FAU - Xie, Bo AU - Xie B AD - Department of Interventional Medicine, The First Affiliated Hospital of Bengbu Medical University, Anhui, China. FAU - Yun, Yaqin AU - Yun Y AD - School of Medical Imaging, Bengbu Medical University, Anhui, China. FAU - Xu, Zhouhua AU - Xu Z AD - School of Nursing, Bengbu Medical University, Anhui, China. FAU - Meng, Yue AU - Meng Y AD - School of Nursing, Bengbu Medical University, Anhui, China. FAU - Peng, Mingxuan AU - Peng M AD - School of Nursing, Bengbu Medical University, Anhui, China. FAU - Xie, Yuqiao AU - Xie Y AD - Faculty of Medicine, Macau University of Science and Technology, Macau, Macao SAR, China. FAU - Zhou, Huiyun AU - Zhou H AD - Department of Interventional Medicine, The First Affiliated Hospital of Bengbu Medical University, Anhui, China. FAU - Xu, Hong AU - Xu H AD - School of Medical Imaging, Bengbu Medical University, Anhui, China. FAU - Xu, Lingjie AU - Xu L AD - Department of Medical Oncology, The First Affiliated Hospital of Bengbu Medical University, Anhui, China. LA - eng PT - Journal Article DEP - 20261007 PL - Switzerland TA - Front Physiol JT - Frontiers in physiology JID - 101549006 PMC - PMC13711031 OTO - NOTNLM OT - autonomic nervous system OT - fatigue OT - heart rate variability OT - hepatocellular carcinoma OT - sleep disturbance COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/07 CRDT- 2026/10/28 05:06 PHST- 2026/06/30 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:06 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - 10.3389/fphys.2026.1923785 [doi] PST - epublish SO - Front Physiol. 2026 Oct 7;17:1923785. doi: 10.3389/fphys.2026.1923785. eCollection 2026. PMID- 42947991 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2673-3439 (Electronic) IS - 2673-3439 (Linking) VI - 8 DP - 2026 TI - Engineering next-generation CAR-NK cells for hematological malignancies: genome editing, synthetic biology, and precision immunotherapy. PG - 1943068 LID - 10.3389/fgeed.2026.1943068 [doi] LID - 1943068 AB - Chimeric antigen receptor (CAR)-based immunotherapy has transformed cancer treatment by enabling engineered immune cells to selectively recognize and eliminate malignant targets, with CAR-T cells establishing the first major clinical success in this field. However, severe associated toxicities, logistical complexities of autologous manufacturing, and high rates of antigen-loss relapse have accelerated the development of alternative effector platforms such as CAR-NK cells. CAR-NK cells combine antigen-directed cytotoxicity with the innate antitumor functions of natural killer cells, offering a potentially safer and more readily available off-the-shelf therapeutic strategy. Nevertheless, the clinical efficacy of CAR-NK therapy is constrained by key biological and translational hurdles: brief in vivo persistence, inadequate trafficking and solid tumor infiltration, severe susceptibility to tumor microenvironment-mediated immunosuppression, and antigen escape driven by tumor heterogeneity. These biological constraints are compounded by technical bottlenecks, including cell source variability, limited large-scale expansion capacity, ex vivo functional exhaustion, and an absence of standardized manufacturing protocols. To address these multi-faceted barriers, recent advances in genome engineering, systems biology, and computational modeling provide promising routes forward. Given that prolonged exposure to immunosuppressive niches induces functional impairment, genome-editing strategies targeting inhibitory checkpoints and persistence programs are crucial; however, enhancing persistence requires stringent safeguards to mitigate toxicity risks from constitutive signaling or genomic instability. Thus, engineering next-generation CAR-NK cells necessitates balancing extended longevity, potent cytotoxicity, and safety. Multiomics approaches facilitate deeper characterization of CAR-NK functional states and tumor-immune interactions, whereas artificial intelligence-guided engineering integrates high-dimensional biological and manufacturing data to optimize receptor design, predict therapeutic performance, and accelerate translational workflows. Collectively, these innovations support a paradigm shift toward programmable CAR-NK cells that can dynamically sense, adapt, and respond to tumor evolution, driving the transition toward autonomous, highly scalable, and precisely controllable cellular immunotherapies in precision oncology. CI - Copyright (c) 2026 Bahr, Basem, Alhejely, Alaa, Abozeid, Basem, Khormi and Abo-Zaid. FAU - Bahr, Ahmed Reda AU - Bahr AR AD - Faculty of Medicine, Alexandria University, Alexandria, Egypt. FAU - Basem, Youssef AU - Basem Y AD - Medical and Pharmaceutical Industrial Biotechnology Department, College of Biotechnology, Misr University for Science and Technology (MUST), Giza, Egypt. FAU - Alhejely, Amani AU - Alhejely A AD - Biology Department, Darb University College, Jazan University, Jazan, Saudi Arabia. FAU - Alaa, Abdel Rahman AU - Alaa AR AD - Medical and Pharmaceutical Industrial Biotechnology Department, College of Biotechnology, Misr University for Science and Technology (MUST), Giza, Egypt. FAU - Abozeid, Saifallah Mabrouk AU - Abozeid SM AD - Faculty of Medicine, Cairo University, Kasr AlAiny, Egypt. FAU - Basem, Amira AU - Basem A AD - Department of Biotechnology and Genetic Engineering, College of Science, Helwan National University, Cairo, Egypt. FAU - Khormi, Mohsen A AU - Khormi MA AD - Department of Biology, College of Science, Jazan University, Jazan, Saudi Arabia. FAU - Abo-Zaid, Mabrouk A AU - Abo-Zaid MA AD - Department of Biology, College of Science, Jazan University, Jazan, Saudi Arabia. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Genome Ed JT - Frontiers in genome editing JID - 101775540 PMC - PMC13710923 OTO - NOTNLM OT - CAR-NK cells OT - CAR-based immunotherapy OT - CRISPR genome editing OT - cancer immunotherapy OT - synthetic biology COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:05 PHST- 2026/07/20 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:05 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 1943068 [pii] AID - 10.3389/fgeed.2026.1943068 [doi] PST - epublish SO - Front Genome Ed. 2026 Oct 13;8:1943068. doi: 10.3389/fgeed.2026.1943068. eCollection 2026. PMID- 42947989 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1663-9812 (Print) IS - 1663-9812 (Electronic) IS - 1663-9812 (Linking) VI - 17 DP - 2026 TI - Phenotype-informed selection of microbiota-directed interventions in irritable bowel syndrome: current evidence and limitations. PG - 1921349 LID - 10.3389/fphar.2026.1921349 [doi] LID - 1921349 AB - Irritable bowel syndrome (IBS) is a heterogeneous disorder of gut-brain interaction in which bowel-habit subtype, dominant symptoms, post-infectious onset, bile-acid handling, visceral sensitivity, and diet-microbiota interactions may influence the treatment response. This narrative mini-review asks whether available clinical evidence is sufficient to select or sequence microbiota-directed and microbiota-modulating interventions according to Rome bowel-habit subtype and clinically observable dominant symptoms. Literature was identified through PubMed/MEDLINE and major guidelines, systematic reviews, meta-analyses, and pivotal trials published through June 2026. Evidence was interpreted at the level of the exact intervention or product, studied population, target outcome, consistency, independent replication, safety, and guideline position. Mechanistic data support plausible links through fermentation, epithelial barrier function, mucosal immunity, bile-acid metabolism, visceral sensitivity, and gut-brain signaling, but mechanistic or microbiome changes do not establish the clinical efficacy. Rifaximin has the clearest replicated and guideline-supported role in IBS with diarrhea, while soluble fiber has guideline support for global or constipation-associated symptoms. Evidence for individual probiotics, postbiotics, partially hydrolyzed guar gum, galactooligosaccharides, and fermented matrices remains product-specific or emerging and is rarely independently replicated. Symptom improvement with a diet low in fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (low-FODMAP diet) does not establish microbiota mediation. Fecal microbiota transplantation (FMT) and microbiome-guided prescribing remain investigational. Current evidence, therefore, supports only limited phenotype-informed matching and does not establish a validated treatment sequence or microbiome-defined therapeutic phenotype. CI - Copyright (c) 2026 Sallem. FAU - Sallem, Om Kolthoum AU - Sallem OK AD - Department of Nutrition, Faculty of Medicine of Monastir, EPS Fattouma Bourguiba, Monastir, Tunisia. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Pharmacol JT - Frontiers in pharmacology JID - 101548923 PMC - PMC13710920 OTO - NOTNLM OT - fecal microbiota transplantation OT - gut microbiota OT - irritable bowel syndrome OT - phenotype-informed care OT - postbiotics OT - prebiotics OT - probiotics OT - rifaximin COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:05 PHST- 2026/06/27 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:05 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 1921349 [pii] AID - 10.3389/fphar.2026.1921349 [doi] PST - epublish SO - Front Pharmacol. 2026 Oct 13;17:1921349. doi: 10.3389/fphar.2026.1921349. eCollection 2026. PMID- 42947985 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2168-8184 (Print) IS - 2168-8184 (Electronic) IS - 2168-8184 (Linking) VI - 18 IP - 9 DP - 2026 Sep TI - Atomoxetine for Orthostatic Hypotension in Traumatic and Non-traumatic Spinal Cord Injury: A Case Series From a Neuro-Rehabilitation Setting. PG - e116985 LID - 10.7759/cureus.116985 [doi] LID - e116985 AB - Orthostatic hypotension (OH) following spinal cord injury (SCI) can significantly limit functional recovery by impairing participation in rehabilitation. Non-pharmacological techniques and pharmacological therapies, including fludrocortisone and midodrine, are conventionally used to manage OH. However, when these measures fail, atomoxetine has demonstrated benefit in other neurogenic OH populations. Evidence regarding the efficacy of this selective norepinephrine reuptake inhibitor is limited in neurogenic OH related to SCI. In this case series, we describe five patients with SCI who had symptomatic OH that did not respond to conventional pharmacological agents but showed clinical improvement with atomoxetine (dose range of 10 mg to 23 mg). Hence, atomoxetine can be considered a potential alternative therapeutic option for OH related to SCI in patients with OH refractory to conventional management. CI - Copyright (c) 2026, Prakash et al. FAU - Prakash, Navin B AU - Prakash NB AD - Department of Neurological Rehabilitation, National Institute of Mental Health and Neuro Sciences, Bangalore, IND. FAU - Pavithran, Shaneena AU - Pavithran S AD - Department of Neurological Rehabilitation, National Institute of Mental Health and Neuro Sciences, Bangalore, IND. FAU - Gupta, Anupam AU - Gupta A AD - Department of Neurological Rehabilitation, National Institute of Mental Health and Neuro Sciences, Bangalore, IND. FAU - Khanna, Meeka AU - Khanna M AD - Department of Neurological Rehabilitation, National Institute of Mental Health and Neuro Sciences, Bangalore, IND. LA - eng PT - Case Reports PT - Journal Article DEP - 20260926 PL - United States TA - Cureus JT - Cureus JID - 101596737 PMC - PMC13710944 OTO - NOTNLM OT - atomoxetine OT - low blood pressure OT - myelopathy OT - orthostatic hypotension OT - spinal cord injury COIS- Human subjects: Informed consent for treatment and open access publication was obtained or waived by all participants in this study. Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/09/26 CRDT- 2026/10/28 05:04 PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:04 [entrez] PHST- 2026/09/26 00:00 [pmc-release] AID - 10.7759/cureus.116985 [doi] PST - epublish SO - Cureus. 2026 Sep 26;18(9):e116985. doi: 10.7759/cureus.116985. eCollection 2026 Sep. PMID- 42947984 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Benchmarking the performance of large language models on medical genetics questions: a comparative evaluation of GPT-5.1, Gemini Pro 3, and Claude Opus 4.5. PG - 1946188 LID - 10.3389/fmed.2026.1946188 [doi] LID - 1946188 AB - OBJECTIVE: This study benchmarks the performance of three state of the art large reasoning models comprising GPT 5.1, Gemini Pro 3, and Claude Opus 4.5 by evaluating their accuracy, response consistency, processing speed, and output length on medical genetics questions from the Turkish Medical Specialty Examination (TUS). METHODS: A total of 233 medical genetics questions were selected based on predefined inclusion criteria covering molecular mechanisms, inheritance patterns, genetic disorders, and diagnostic techniques. Each question was independently submitted to GPT 5.1, Gemini Pro 3, and Claude Opus 4.5 using a standardized prompt. Model accuracy was compared using McNemar's test. Response consistency was assessed using Cohen's Kappa. Response time and word count were analyzed using the Friedman test with Dunn Bonferroni post hoc comparisons. RESULTS: No statistically significant differences were observed in overall accuracy among the models (p > 0.05). GPT 5.1 achieved the highest accuracy at 97.9 percent followed by Claude Opus 4.5 at 96.6 percent and Gemini Pro 3 at 96.1 percent. Cohen's Kappa indicated moderate qualitative divergence between Gemini Pro 3 and Claude Opus 4.5 with kappa equal to 0.451. Significant differences were found in response time and output length (p < 0.001). CONCLUSION: Although all models demonstrate similarly high accuracy, they differ in operational behavior, suggesting distinct internal knowledge representations and response strategies. CI - (c) 2026 Basdemirci and Tassoker. FAU - Basdemirci, Muserref AU - Basdemirci M AD - Department of Medical Genetics, Konya City Hospital, Konya, Turkiye. FAU - Tassoker, Melek AU - Tassoker M AD - Department of Dentomaxillofacial Radiology, Faculty of Dentistry, Necmettin Erbakan University, Konya, Turkiye. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13710261 OTO - NOTNLM OT - artificial intelligence OT - benchmarking OT - chatGPT OT - genetics OT - language models OT - medical COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:04 PHST- 2026/07/23 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:04 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fmed.2026.1946188 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 13;13:1946188. doi: 10.3389/fmed.2026.1946188. eCollection 2026. PMID- 42947983 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2577-171X (Electronic) IS - 2577-171X (Linking) VI - 9 IP - 6 DP - 2026 Nov TI - Primary Prostatic Diffuse Large B-Cell Lymphoma Mimicking Prostate Cancer With a Normal Prostate-Specific Antigen Level. PG - e70286 LID - 10.1002/iju5.70286 [doi] LID - e70286 AB - INTRODUCTION: Primary prostatic lymphoma is rare and may mimic prostate cancer clinically and radiologically. CASE PRESENTATION: A 66-year-old man was referred for evaluation of suspected advanced prostate cancer. Despite a normal prostate-specific antigen level of 0.573 ng/mL, imaging revealed a prostatic mass with suspected bladder invasion and bilateral hydronephrosis. Prostate needle biopsy showed atypical lymphocytic infiltration but could not distinguish lymphoma from inflammatory changes. Additional transurethral biopsy provided sufficient tissue for histopathological and immunohistochemical evaluation, establishing a diagnosis of primary prostatic diffuse large B-cell lymphoma. CONCLUSION: Primary prostatic lymphoma should be considered when clinical and imaging findings suggest prostate cancer despite a normal prostate-specific antigen level. Additional tissue sampling should be considered when needle biopsy is inconclusive despite persistent clinical suspicion of lymphoma. CI - (c) 2026 The Author(s). IJU Case Reports published by John Wiley & Sons Australia, Ltd on behalf of Japanese Urological Association. FAU - Amamoto, Kota AU - Amamoto K AUID- ORCID: 0009-0007-1698-9932 AD - Department of Urology Nagasaki University Graduate School of Biomedical Sciences Nagasaki Japan. FAU - Nakamura, Yuichiro AU - Nakamura Y AUID- ORCID: 0009-0002-3723-8058 AD - Department of Urology Nagasaki University Graduate School of Biomedical Sciences Nagasaki Japan. FAU - Fukushima, Hajime AU - Fukushima H AD - Department of Urology Nagasaki University Graduate School of Biomedical Sciences Nagasaki Japan. FAU - Harada, Junki AU - Harada J AUID- ORCID: 0000-0003-0721-7870 AD - Department of Urology Nagasaki University Graduate School of Biomedical Sciences Nagasaki Japan. FAU - Matsuda, Tsuyoshi AU - Matsuda T AUID- ORCID: 0009-0002-5256-074X AD - Department of Urology Nagasaki University Graduate School of Biomedical Sciences Nagasaki Japan. FAU - Ito, Itsuho AU - Ito I AD - Department of Urology Nagasaki University Graduate School of Biomedical Sciences Nagasaki Japan. FAU - Mitsunari, Kensuke AU - Mitsunari K AUID- ORCID: 0000-0002-6963-5632 AD - Department of Urology Nagasaki University Graduate School of Biomedical Sciences Nagasaki Japan. FAU - Matsuo, Tomohiro AU - Matsuo T AUID- ORCID: 0000-0002-9508-0319 AD - Department of Urology Nagasaki University Graduate School of Biomedical Sciences Nagasaki Japan. FAU - Ohba, Kojiro AU - Ohba K AUID- ORCID: 0000-0003-2841-1998 AD - Department of Urology Nagasaki University Graduate School of Biomedical Sciences Nagasaki Japan. FAU - Imamura, Ryoichi AU - Imamura R AUID- ORCID: 0000-0002-8782-9852 AD - Department of Urology Nagasaki University Graduate School of Biomedical Sciences Nagasaki Japan. LA - eng PT - Journal Article DEP - 20261026 PL - Australia TA - IJU Case Rep JT - IJU case reports JID - 101764958 PMC - PMC13711019 OTO - NOTNLM OT - biopsy OT - large B-cell lymphoma OT - prostate OT - prostate-specific antigen OT - prostatic neoplasms COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/26 CRDT- 2026/10/28 05:04 PHST- 2026/08/24 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:04 [entrez] PHST- 2026/10/26 00:00 [pmc-release] AID - IJU570286 [pii] AID - 10.1002/iju5.70286 [doi] PST - epublish SO - IJU Case Rep. 2026 Oct 26;9(6):e70286. doi: 10.1002/iju5.70286. eCollection 2026 Nov. PMID- 42947980 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-4185 (Print) IS - 2296-4185 (Electronic) IS - 2296-4185 (Linking) VI - 14 DP - 2026 TI - Multi-scale computational modeling of magnetoelectric nanoparticle-dispersed conductive nanofibers for peripheral nerve modulation. PG - 1951247 LID - 10.3389/fbioe.2026.1951247 [doi] LID - 1951247 AB - INTRODUCTION: The electrical stimulation of peripheral nerves has shown the potential to restore impaired functions across a wide range of clinical scenarios. In most cases, though, the lack of selectivity of existing stimulation interfaces limits their applicability and can in some cases produce severe adverse effects. In addition, traditional stimulation requires physical connectors to a pulse generator that can add further biological and mechanical stress already linked to interfacing with physiological structures. This study focuses on the use of magnetoelectric nanoparticles (MENPs) embedded within conductive polymeric nanofibers as new wireless, highly selective, and minimally invasive interfaces for modulating peripheral nerves activity. METHODS: A step-by-step detailed characterization of the physical variables involved in this interaction, spanning from the nanometric single MENPs to micrometric multi-nanofibers structure with dispersed MENPs (poly-ME devices) and ending with the millimetric peripheral nerve, was proposed. MENPs concentration and distribution, device geometry, orientation, and size were systematically varied, and their effects on nerve fiber recruitment were quantified across myelinated and unmyelinated fiber types at different distances from the stimulating source. RESULTS AND DISCUSSION: Our results evidenced how higher MENPs concentrations, higher stimulating source size and lower distance enhance the modulation capability of the poly-ME devices, with differences in stimulation capability between myelinated and unmyelinated nerve fibers. The solution of embedding MENPs in conductive polymer nanofibers enables higher MENPs loading than direct tissue injection, improves positional stability, and preserves biocompatibility, thereby offering a potential alternative for the engineering of MENPs as neuromodulation interfaces. CI - Copyright (c) 2026 Galletta, Fiocchi, Verardo, Carbone, del Mercato, Romeni and Chiaramello. FAU - Galletta, Valentina AU - Galletta V AD - Department of Electronics, Information and Bioengineering (DEIB), Politecnico di Milano, Milan, Italy. AD - Institute of Electronics, Computer and Telecommunication Engineering (IEIIT), National Research Council (CNR), Milan, Italy. FAU - Fiocchi, Serena AU - Fiocchi S AD - Institute of Electronics, Computer and Telecommunication Engineering (IEIIT), National Research Council (CNR), Milan, Italy. FAU - Verardo, Claudio AU - Verardo C AD - The BioRobotics institute, Health Science Interdisciplinary Research Center, and Department of Excellence Robotics and AI, Scuola Superiore Sant'anna, Pisa, Italy. FAU - Carbone, Luigi AU - Carbone L AD - Institute of Nanotechnology, National Research Council (CNR NANOTEC), Lecce, Italy. AD - Istituto Italiano di Tecnologia, Center for Biomolecular Nanotechnologies, Arnesano (LE), Italy. FAU - Del Mercato, Loretta Laureana AU - Del Mercato LL AD - Institute of Nanotechnology, National Research Council (CNR NANOTEC), Lecce, Italy. AD - Tecnomed Puglia - Technopole for Precision Medicine (Biotech Lecce Hub), Lecce, Italy. FAU - Romeni, Simone AU - Romeni S AD - Modular Implantable Neurotechnologies (MINE) Laboratory, Universita Vita Salute San Raffaele and Scuola Superiore Sant'Anna, Milan, Italy. FAU - Chiaramello, Emma AU - Chiaramello E AD - Institute of Electronics, Computer and Telecommunication Engineering (IEIIT), National Research Council (CNR), Milan, Italy. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Bioeng Biotechnol JT - Frontiers in bioengineering and biotechnology JID - 101632513 PMC - PMC13710225 OTO - NOTNLM OT - computational modelling OT - core-shell magnetoelectric nanoparticles OT - hybrid biointerfaces OT - noninvasive stimulation OT - wireless peripheral nervous stimulation COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:04 PHST- 2026/07/28 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:04 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 1951247 [pii] AID - 10.3389/fbioe.2026.1951247 [doi] PST - epublish SO - Front Bioeng Biotechnol. 2026 Oct 13;14:1951247. doi: 10.3389/fbioe.2026.1951247. eCollection 2026. PMID- 42947977 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2234-943X (Print) IS - 2234-943X (Electronic) IS - 2234-943X (Linking) VI - 16 DP - 2026 TI - Survey-based perspectives and reported barriers to DPYD testing among U.S. oncologists. PG - 1903512 LID - 10.3389/fonc.2026.1903512 [doi] LID - 1903512 AB - BACKGROUND: Patients with cancer with deleterious DPYD variants are at significantly increased risk for severe toxicity with standard fluoropyrimidine doses. Prior to recent guidelines recommending it, the uptake of pre-treatment DPYD testing in the United States has been low. We aimed to explore oncologists' perceptions and barriers to DPYD testing adoption on a national level. MATERIALS AND METHODS: Survey invitations were sent to U.S. oncologists listed in the American Medical Association (AMA) Masterfile in April and May 2025. The surveys assessed physician knowledge, attitudes, and perceptions of DPYD testing in clinics caring for patients with cancer in the United States. Descriptive statistics were used to evaluate survey responses. RESULTS: Of 5,525 physicians invited to participate, 47 oncologists completed the survey (response rate: 0.85%). The majority (85%) indicated that DPYD testing could help them manage patients; however, only 34% reported routinely performing DPYD testing. Most physicians (77%) agreed that adequate resources were available to order DPYD testing. When directly asked, 36% indicated that limited laboratory access was a barrier. In open-ended responses, respondents identified insurance reimbursement concerns (21%) and prolonged turnaround time (19%) as additional barriers. Forty-three percent reported needing assistance interpreting results and applying them to drug selection and dosing. Webinars (25.5%) and self-paced online modules (21%) were the most preferred educational modalities. CONCLUSION: Most respondents indicated that DPYD testing could help manage patients, yet concerns persist. While regulatory guidance is evolving, these preliminary findings from a small sample highlight the need for future research to characterize implementation barriers and inform policy development. IMPLICATIONS FOR PRACTICE: Our findings highlight gaps in implementation that can inform targeted strategies to improve the integration of DPYD testing into oncology practice, with the potential to enhance patient safety and reduce fluoropyrimidine-related toxicity. CI - Copyright (c) 2026 Tohme, Salloum, De La Cruz, Lu, Dong, Hicks, Smith, DeRemer, Mroz, Tuteja and Cavallari. FAU - Tohme, Sara AU - Tohme S AD - Department of Health Outcomes and Biomedical Informatics, University of Florida, Gainesville, FL, United States. FAU - Salloum, Ramzi G AU - Salloum RG AD - Department of Health Outcomes and Biomedical Informatics, University of Florida, Gainesville, FL, United States. FAU - De La Cruz, Jacqueline AU - De La Cruz J AD - Department of Health Outcomes and Biomedical Informatics, University of Florida, Gainesville, FL, United States. FAU - Lu, Qing AU - Lu Q AD - Department of Biostatistics, University of Florida, Gainesville, FL, United States. FAU - Dong, Kejin AU - Dong K AD - Department of Biostatistics, University of Florida, Gainesville, FL, United States. FAU - Hicks, J Kevin AU - Hicks JK AD - Departments of Pathology and Translational Pathology, Moffitt Cancer Center, Tampa, FL, United States. FAU - Smith, D Max AU - Smith DM AD - MedStar Health, Columbia, MD, United States. FAU - DeRemer, David AU - DeRemer D AD - Department of Pharmacotherapy and Translational Research and Center for Pharmacogenomics and Precision Medicine, University of Florida, Gainesville, FL, United States. FAU - Mroz, Pawel AU - Mroz P AD - Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN, United States. FAU - Tuteja, Sony AU - Tuteja S AD - Division of Translational Medicine and Human Genetics, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, United States. FAU - Cavallari, Larisa H AU - Cavallari LH AD - Department of Pharmacotherapy and Translational Research and Center for Pharmacogenomics and Precision Medicine, University of Florida, Gainesville, FL, United States. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Oncol JT - Frontiers in oncology JID - 101568867 PMC - PMC13710826 OTO - NOTNLM OT - drug-related side effects and adverse reactions OT - implementation science OT - medical oncology OT - pharmacogenetic testing OT - precision medicine COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:03 PHST- 2026/06/08 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:03 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fonc.2026.1903512 [doi] PST - epublish SO - Front Oncol. 2026 Oct 13;16:1903512. doi: 10.3389/fonc.2026.1903512. eCollection 2026. PMID- 42947971 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-634X (Print) IS - 2296-634X (Electronic) IS - 2296-634X (Linking) VI - 14 DP - 2026 TI - PP2A targeting: balancing anticancer potential and neurological risk. PG - 1945801 LID - 10.3389/fcell.2026.1945801 [doi] LID - 1945801 AB - Protein phosphatase 2A (PP2A) is a multifunctional serine/threonine phosphatase that regulates cell-cycle progression, oncogenic signaling, neuronal development, synaptic homeostasis, and protein proteostasis. Its dysregulation contributes to cancer, whereas impaired PP2A activity in the nervous system is linked to neurodevelopmental disorders and neurodegenerative proteinopathies, including tau and alpha-synuclein pathology. These divergent roles create both opportunities and safety challenges for PP2A-directed therapies. This review examines PP2A as a modular holoenzyme system whose subunit composition determines substrate specificity and context-dependent biological effects. We discuss PP2A dysfunction in cancer, including genetic and regulatory alterations affecting PP2A subunits, assembly, and endogenous inhibitors, and compare therapeutic PP2A activation with transient inhibition. The latter approach is exemplified by LB100, which sensitizes treatment-stressed tumor cells to chemotherapy, radiotherapy, or immunotherapy by disrupting checkpoint adaptation and stress recovery. We further evaluate the neurological implications of systemic phosphatase inhibition, LB100 conversion to the active metabolite endothall, clinical safety and pharmacokinetic data, and the limited brain-tumor penetration of intact LB100. We propose that the clinical utility of PP2A inhibition depends on defining a therapeutic corridor that integrates dosing schedule, parent-drug and metabolite exposure, tumor target engagement, biomarker-informed patient selection, and preservation of PP2A-dependent homeostasis in normal tissues, particularly the nervous system. CI - Copyright (c) 2026 Chigriai, Kraskovskaya, Bolshakova, Porozova, Porozov and Pesic. FAU - Chigriai, Margarita AU - Chigriai M AD - Advitam Laboratory, Belgrade, Serbia. FAU - Kraskovskaya, Nina AU - Kraskovskaya N AD - Institute of Cytology RAS, St. Petersburg, Russia. FAU - Bolshakova, Anastasia AU - Bolshakova A AD - Advitam Laboratory, Belgrade, Serbia. FAU - Porozova, Natalia O AU - Porozova NO AD - Advitam Laboratory, Belgrade, Serbia. AD - The Laboratory of Bio- and Chemoinformatics, School of Computer Science, Physics and Technology, HSE University, St. Petersburg, Russia. FAU - Porozov, Yuri B AU - Porozov YB AD - The Laboratory of Bio- and Chemoinformatics, School of Computer Science, Physics and Technology, HSE University, St. Petersburg, Russia. FAU - Pesic, Milica AU - Pesic M AD - Department of Neurobiology, Institute for Biological Research "Sinisa Stankovic" - National Institute of the Republic of Serbia, University of Belgrade, Belgrade, Serbia. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Cell Dev Biol JT - Frontiers in cell and developmental biology JID - 101630250 PMC - PMC13710974 OTO - NOTNLM OT - Alzheimer's disease OT - LB100 OT - PP2A OT - cancer OT - neurodegeneration OT - neurodevelopment OT - oncogenic signaling COIS- MC, AB, and NP were employed by company: Advitam Laboratory, Belgrade, Serbia. The remaining author(s) declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author MP declared that they were an editorial board member of Frontiers at the time of submission. This had no impact on the peer review process and the final decision. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:03 PHST- 2026/07/22 00:00 [received] PHST- 2026/09/12 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:03 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 1945801 [pii] AID - 10.3389/fcell.2026.1945801 [doi] PST - epublish SO - Front Cell Dev Biol. 2026 Oct 13;14:1945801. doi: 10.3389/fcell.2026.1945801. eCollection 2026. PMID- 42947970 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1662-5099 (Print) IS - 1662-5099 (Electronic) IS - 1662-5099 (Linking) VI - 19 DP - 2026 TI - Depressive phenotype shapes behavioral, neuroendocrine and molecular responses to repetitive transcranial magnetic stimulation in preclinical models of depression. PG - 1939025 LID - 10.3389/fnmol.2026.1939025 [doi] LID - 1939025 AB - INTRODUCTION: Repetitive transcranial magnetic stimulation (rTMS) is an established non-invasive treatment for treatment-resistant depression (TRD), yet its neurobiological mechanisms remain only partially understood. We investigated whether rTMS recruits common or phenotype-specific adaptive mechanisms across depressive conditions. METHODS: Male Wistar-Kyoto rats exposed to chronic mild stress and social isolation modeled TRD. Socially isolated Wistar rats modeled treatment-responsive depression (ISO), while socially housed Wistar rats served as non-depressed controls (CTRL). Animals received active or sham high-frequency rTMS. Behavioral batteries assessing motivation, emotionality, helplessness, and anxiety-like behavior began 1 and 14 days after the final rTMS session. Basal plasma corticosterone and Bdnf and Cnr1 mRNA expression in the prefrontal cortex and hippocampus were also assessed. RESULTS: rTMS produced phenotype-dependent behavioral, neuroendocrine, and molecular effects. In ISO rats, active stimulation improved multiple behavioral measures and reduced basal corticosterone. In TRD rats, behavioral effects were more selective, primarily involving motivation and self-care, and occurred without corticosterone normalization. The most prominent molecular effects emerged in the prefrontal cortex of TRD rats, where rTMS increased both Bdnf and Cnr1 expression. Their expression was strongly positively correlated only in active TRD animals, suggesting coordinated engagement of plasticity-related pathways. CONCLUSION: rTMS did not elicit a uniform response across depressive phenotypes but recruited distinct adaptive mechanisms according to the underlying neurobiological state. Neuroendocrine normalization characterized the treatment-responsive model, whereas selective behavioral improvement in TRD was associated with coordinated engagement of prefrontal Bdnf- and Cnr1-related pathways. These findings support phenotype-informed, mechanism-guided neuromodulation strategies for depression. CI - Copyright (c) 2026 Boffa, Spalletti, Martella, Cerri, Feliciani, Inserra, De Risio, Di Lorenzo, Martinotti, D'Addario, Pettorruso and Zoratto. FAU - Boffa, Marta AU - Boffa M AD - Centre for Behavioural Sciences and Mental Health, Istituto Superiore di Sanita, Rome, Italy. AD - Department of Neuroscience, Imaging and Clinical Sciences, "G. d'Annunzio" University of ChietiPescara, Chieti, Italy. FAU - Spalletti, Sara AU - Spalletti S AD - Centre for Behavioural Sciences and Mental Health, Istituto Superiore di Sanita, Rome, Italy. AD - Department of Neuroscience, Imaging and Clinical Sciences, "G. d'Annunzio" University of ChietiPescara, Chieti, Italy. FAU - Martella, Francesca AU - Martella F AD - Department of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy. FAU - Cerri, Carola AU - Cerri C AD - Centre for Behavioural Sciences and Mental Health, Istituto Superiore di Sanita, Rome, Italy. FAU - Feliciani, Beatrice AU - Feliciani B AD - Department of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy. FAU - Inserra, Antonio AU - Inserra A AD - Centre for Behavioural Sciences and Mental Health, Istituto Superiore di Sanita, Rome, Italy. AD - Department of Neuroscience, Imaging and Clinical Sciences, "G. d'Annunzio" University of ChietiPescara, Chieti, Italy. FAU - De Risio, Luisa AU - De Risio L AD - Department of Mental Health and Addiction, ASL Roma 5, Rome, Italy. FAU - Di Lorenzo, Giorgio AU - Di Lorenzo G AD - Laboratory of Psychophysiology and Cognitive Neuroscience, Department of Systems Medicine, University of Rome Tor Vergata, Rome, Italy. AD - IRCCS Fondazione Santa Lucia, Rome, Italy. FAU - Martinotti, Giovanni AU - Martinotti G AD - Department of Neuroscience, Imaging and Clinical Sciences, "G. d'Annunzio" University of ChietiPescara, Chieti, Italy. AD - Department of Mental Health, ASL 2 Abruzzo Lanciano-Vasto-Chieti, Chieti, Italy. AD - Psychopharmacology, Drug Misuse and Novel Psychoactive Substances Research Unit, School of Life and Medical Sciences, University of Hertfordshire, Hatfield, United Kingdom. FAU - D'Addario, Claudio AU - D'Addario C AD - Department of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy. FAU - Pettorruso, Mauro AU - Pettorruso M AD - Department of Neuroscience, Imaging and Clinical Sciences, "G. d'Annunzio" University of ChietiPescara, Chieti, Italy. AD - Department of Mental Health, ASL 2 Abruzzo Lanciano-Vasto-Chieti, Chieti, Italy. FAU - Zoratto, Francesca AU - Zoratto F AD - Centre for Behavioural Sciences and Mental Health, Istituto Superiore di Sanita, Rome, Italy. LA - eng SI - figshare/10.6084/m9.figshare.33477346 PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Mol Neurosci JT - Frontiers in molecular neuroscience JID - 101477914 PMC - PMC13710254 OTO - NOTNLM OT - BDNF OT - endocannabinoid system OT - neuromodulation OT - neuroplasticity OT - preclinical model OT - repetitive transcranial magnetic stimulation OT - treatment-resistant depression COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:02 PHST- 2026/07/15 00:00 [received] PHST- 2026/08/24 00:00 [revised] PHST- 2026/08/31 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:02 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fnmol.2026.1939025 [doi] PST - epublish SO - Front Mol Neurosci. 2026 Oct 13;19:1939025. doi: 10.3389/fnmol.2026.1939025. eCollection 2026. PMID- 42947969 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-889X (Print) IS - 2296-889X (Electronic) IS - 2296-889X (Linking) VI - 13 DP - 2026 TI - Spatial lipidomics in gynecologic malignancies: molecular identification, cellular attribution, and mechanistic validation. PG - 1957466 LID - 10.3389/fmolb.2026.1957466 [doi] LID - 1957466 AB - Spatial lipidomics can place lipid identities within histopathologic compartments, but ion maps are frequently interpreted beyond what their analytical and biological validation can support. Here, we critically synthesize direct spatial lipid evidence in ovarian, endometrial, and cervical cancers and use spatial metabolomics, mass spectrometry imaging, spatial transcriptomics or proteomics, and non-spatial lipid biology only as explicitly graded support. We organize inference as an eight-gate chain: pathology-defined sampling, analytical quality control, structural identification, cellular attribution, metabolic flux, perturbation and rescue, patient-level validation, and prospective clinical utility. Within this framework, lipid uptake, de novo synthesis, desaturation, fatty-acid oxidation, membrane remodeling, prostaglandin E2 signaling, and ferroptosis are interpreted as mechanistic programs downstream of spatially anchored observations. Disease ecology determines which programs are plausible: omental adipocyte-tumor exchange dominates ovarian cancer, obesity-estrogen-insulin and PI3K signaling frame endometrial cancer, and persistent high-risk human papillomavirus infection shapes membrane and inflammatory remodeling in cervical cancer. Evidence remains highly asymmetric. Ovarian cancer has the broadest spatial literature; endometrial evidence relies principally on one 64-patient DESI-MSI cohort; and cervical evidence is limited to very small phospholipid and oleate studies. Spatial lipidomics is therefore ready to localize disease-specific niches, prioritize causal experiments, and define testable patient subsets, but not yet to support routine screening, diagnosis, treatment selection, or surveillance. Closing the gap requires orthogonal structural confirmation, cell-resolved source assignment, isotope tracing, functional rescue, and locked multicenter validation. CI - Copyright (c) 2026 Zhao and Ma. FAU - Zhao, Jingyan AU - Zhao J AD - Medical Department, Northwest Minzu University, Lanzhou, Gansu Province, China. FAU - Ma, Wei AU - Ma W AD - Medical Department, Northwest Minzu University, Lanzhou, Gansu Province, China. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Mol Biosci JT - Frontiers in molecular biosciences JID - 101653173 PMC - PMC13710824 OTO - NOTNLM OT - ferroptosis OT - gynecologic malignancies OT - lipid metabolism OT - mass spectrometry imaging OT - metabolomics OT - spatial lipidomics OT - tumor microenvironment COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:02 PHST- 2026/08/03 00:00 [received] PHST- 2026/09/11 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:02 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 1957466 [pii] AID - 10.3389/fmolb.2026.1957466 [doi] PST - epublish SO - Front Mol Biosci. 2026 Oct 13;13:1957466. doi: 10.3389/fmolb.2026.1957466. eCollection 2026. PMID- 42947968 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2331-8325 (Electronic) IS - 2331-8325 (Linking) VI - 16 IP - 20 DP - 2026 Oct 20 TI - An Improved Method for Rapid Unmarked Gene Deletion in Bacteria: Pseudomonas aeruginosa as an Example. PG - e5847 LID - 10.21769/BioProtoc.5847 [doi] LID - e5847 AB - The release and updating of whole-genome sequences of several representatives of the human opportunistic pathogen Pseudomonas aeruginosa have laid the ground for investigating the mechanisms of antibiotic resistance, biofilm formation, and virulence, while also offering opportunities for researchers to find new therapeutic targets to control this bacterium using a functional genomics approach. However, there is still a lack of detailed protocols describing gene inactivation methods in P. aeruginosa, resulting in failures and extra time spent designing in-house protocols. Here, we introduce a rapid, efficient, and unmarked deletion mutagenesis method combining overlap extension PCR, efficient conjugation, and the traditionally used sacB-based counter-selection procedure. Efficient generation of deletion mutants using this detailed protocol can be easily completed in one week using standard lab reagents. Importantly, this method may be adaptable to other bacteria where the sacB-based counter-selection system works. Key features * Using modified overlap extension PCR (MOE-PCR), the deletion construct can be generated rapidly in a single fusion PCR without intermediate gel purification. * Only one transformation step is involved in the whole protocol, thus saving time compared to marked mutagenesis involving Flp (flippase) recombinase recognition target (FRT)-mediated resolution. * Pseudomonas agar P medium (PM) and casamino acid medium (CAA) reduce reactive oxygen species (ROS)-mediated toxicity, facilitating deletion of oxidative stress-sensitive genes. * The sacB-based counter-selection works in many Gram-negative bacteria and even some Gram-positive actinobacteria, so the protocol may be adaptable to such species. CI - (c)Copyright : (c) 2026 The Authors; This is an open access article under the CC BY-NC license. FAU - Zhang, Junlu AU - Zhang J AD - School of Basic Medicine, Hubei University of Chinese Medicine, Wuhan, China. AD - Engineering Research Center of TCM Protection Technology and New Product Development for the Elderly Brain Health, Ministry of Education, Wuhan, China. FAU - Wang, Ping AU - Wang P AD - Engineering Research Center of TCM Protection Technology and New Product Development for the Elderly Brain Health, Ministry of Education, Wuhan, China. AD - Institute of Geriatrics, Hubei University of Chinese Medicine, Key Laboratory of Alzheimer's disease, State Administration of Traditional Chinese Medicine, Wuhan, China. FAU - Zhang, Xin AU - Zhang X AD - Shanghai Cinopath Medical Laboratory Co., Kindstar Globalgene Technology Inc., Shanghai, China. FAU - Wei, Qing AU - Wei Q AD - Shanghai Cinopath Medical Laboratory Co., Kindstar Globalgene Technology Inc., Shanghai, China. AD - Kindstar Global Precision Medicine Institute, Wuhan, China. FAU - Cui, Kaiyu AU - Cui K AD - Engineering Research Center of TCM Protection Technology and New Product Development for the Elderly Brain Health, Ministry of Education, Wuhan, China. AD - Faculty of Pharmacy, Hubei Shizhen Laboratory, Hubei University of Chinese Medicine, Wuhan, China. LA - eng PT - Journal Article DEP - 20261020 PL - United States TA - Bio Protoc JT - Bio-protocol JID - 101635102 PMC - PMC13710123 OTO - NOTNLM OT - Allelic exchange OT - Gene deletion OT - MOE-PCR OT - Overlap extension PCR OT - P. aeruginosa OT - Unmarked mutagenesis OT - sacB counter-selection COIS- Competing interestsThe authors declare that they have no competing financial interests. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/20 CRDT- 2026/10/28 05:02 PHST- 2026/07/07 00:00 [received] PHST- 2026/09/10 00:00 [revised] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:02 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - e5847 [pii] AID - 5847 [pii] AID - 10.21769/BioProtoc.5847 [doi] PST - epublish SO - Bio Protoc. 2026 Oct 20;16(20):e5847. doi: 10.21769/BioProtoc.5847. eCollection 2026 Oct 20. PMID- 42947967 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2045-7758 (Print) IS - 2045-7758 (Electronic) IS - 2045-7758 (Linking) VI - 16 IP - 10 DP - 2026 Oct TI - Characterization of the First Complete Plastome of Zingiber emeiense: Insights Into Its Phylogenetic Position. PG - e74465 LID - 10.1002/ece3.74465 [doi] LID - e74465 AB - Zingiber emeiense Z. Y. Zhu is an endemic medicinal species restricted to Mount Emei in southwestern China. However, the lack of genomic resources has led to its frequent misclassification and has impeded evolutionary studies. Here, we assembled and characterized the first two complete chloroplast genomes (plastomes) of Z. emeiense. The two plastomes were identical in sequence and structure, each with a total length of 163,865 bp and exhibiting a typical quadripartite structure. Each plastome comprised a large single-copy (LSC) region of 88,191 bp, a small single-copy (SSC) region of 15,750 bp, and a pair of inverted repeats (IR) of 29,962 bp each. Each genome encoded 134 functional genes, including 86 protein-coding genes, 40 tRNA genes, and eight rRNA genes, with an overall GC content of 36.04%. Comparative plastome analyses revealed that IR boundary variation and repeat sequence differences were major sources of genome size variation within Zingiber. Seven hypervariable regions (psbM, trnT-UGU, ycf1, petA-psbJ, ndhF, psaC-ndhE, and rps19) were identified as potential molecular markers. Likelihood-based selection analyses revealed that plastid gene evolution was primarily driven by purifying selection, but ten genes showed signatures of positive selection; ycf1, petD, and rpl2 exhibited lineage-specific positive selection on the Z. emeiense clade. Phylogenomic reconstruction based on 70 complete plastomes placed Z. emeiense within sect. Cryptanthium, where it was strongly supported as a sister clade to Zingiber smilesianum and Zingiber teres. This study provides essential genomic resources for conservation and taxonomic studies of this endemic species. CI - (c) 2026 The Author(s). Ecology and Evolution published by British Ecological Society and John Wiley & Sons Ltd. FAU - Chen, Hualan AU - Chen H AD - Chongqing Engineering Research Center for Horticultural Plant, College of Smart Agriculture Chongqing University of Arts and Sciences Chongqing China. FAU - Wang, Zhaofei AU - Wang Z AD - Chongqing Engineering Research Center for Horticultural Plant, College of Smart Agriculture Chongqing University of Arts and Sciences Chongqing China. FAU - Ni, Jinyong AU - Ni J AD - Chongqing Engineering Research Center for Horticultural Plant, College of Smart Agriculture Chongqing University of Arts and Sciences Chongqing China. FAU - Feng, Qinxi AU - Feng Q AD - Chongqing Engineering Research Center for Horticultural Plant, College of Smart Agriculture Chongqing University of Arts and Sciences Chongqing China. FAU - Liu, Yue AU - Liu Y AD - Chongqing Engineering Research Center for Horticultural Plant, College of Smart Agriculture Chongqing University of Arts and Sciences Chongqing China. FAU - Jiang, Lihui AU - Jiang L AD - Chongqing Engineering Research Center for Horticultural Plant, College of Smart Agriculture Chongqing University of Arts and Sciences Chongqing China. AD - Chongqing Key Laboratory for Germplasm Innovation of Special Aromatic Spice Plants, College of Smart, Agriculture Chongqing University of Arts and Sciences Chongqing China. FAU - Song, Chao AU - Song C AD - Chongqing Engineering Research Center for Horticultural Plant, College of Smart Agriculture Chongqing University of Arts and Sciences Chongqing China. AD - Chongqing Key Laboratory for Germplasm Innovation of Special Aromatic Spice Plants, College of Smart, Agriculture Chongqing University of Arts and Sciences Chongqing China. FAU - Zhu, Yanlin AU - Zhu Y AD - Chongqing Engineering Research Center for Horticultural Plant, College of Smart Agriculture Chongqing University of Arts and Sciences Chongqing China. FAU - Zhang, Menghan AU - Zhang M AD - Chongqing Engineering Research Center for Horticultural Plant, College of Smart Agriculture Chongqing University of Arts and Sciences Chongqing China. FAU - Xie, Qin AU - Xie Q AD - Chongqing Engineering Research Center for Horticultural Plant, College of Smart Agriculture Chongqing University of Arts and Sciences Chongqing China. FAU - Xia, Maoqin AU - Xia M AUID- ORCID: 0000-0001-8543-9116 AD - Chongqing Engineering Research Center for Horticultural Plant, College of Smart Agriculture Chongqing University of Arts and Sciences Chongqing China. AD - Chongqing Key Laboratory for Germplasm Innovation of Special Aromatic Spice Plants, College of Smart, Agriculture Chongqing University of Arts and Sciences Chongqing China. FAU - Li, Honglei AU - Li H AD - Chongqing Engineering Research Center for Horticultural Plant, College of Smart Agriculture Chongqing University of Arts and Sciences Chongqing China. AD - Chongqing Key Laboratory for Germplasm Innovation of Special Aromatic Spice Plants, College of Smart, Agriculture Chongqing University of Arts and Sciences Chongqing China. LA - eng PT - Journal Article DEP - 20261025 PL - England TA - Ecol Evol JT - Ecology and evolution JID - 101566408 PMC - PMC13710891 OTO - NOTNLM OT - Zingiber OT - Zingiber emeiense OT - phylogenomics OT - plastome COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/25 CRDT- 2026/10/28 05:02 PHST- 2026/05/07 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:02 [entrez] PHST- 2026/10/25 00:00 [pmc-release] AID - ECE374465 [pii] AID - 10.1002/ece3.74465 [doi] PST - epublish SO - Ecol Evol. 2026 Oct 25;16(10):e74465. doi: 10.1002/ece3.74465. eCollection 2026 Oct. PMID- 42947963 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2639-5274 (Electronic) IS - 2639-5274 (Linking) VI - 9 DP - 2026 TI - Positioning Creation of Cell Communication Channels via Chinese Microneedle. PG - 1459 LID - 10.34133/research.1459 [doi] LID - 1459 AB - The core dilemma of senescence mitigation lies in the disrupted and even detrimental communication between senescent cells and local immune cells. Existing delivery methods cannot precisely establish protective intercellular communication channels across cell types within the enclosed lesion cavity. In this study, we utilize Chinese microneedle technology for precise, minimally invasive localization of aged lesions, and to release spatially targeted and temporally sustained dual-field to create intercellular communication channels between macrophages and senescent cells. The unique physical properties of this Chinese microneedle system confine the current path to specific spatiotemporal windows, releasing an electro-field while simultaneously activating dynamic van der Waals forces that release functionalized hydrogel microparticles, thereby superimposing a vita-field. Within the dual-field active zone, the electro-field not only attenuates cellular senescence but also forms a communication channel from senescent cells to macrophages, driving macrophages into a hyperactivated state. Meanwhile, the vita-field stabilizes the macrophage membrane, enabling long-term, stable communication between hyperactivated macrophages and senescent cells. Ultimately, the intercellular communication channels established by this Chinese microneedle system alleviate cellular senescence and apoptosis, supporting the repair of aged lesions. CI - Copyright (c) 2026 Yiwen Xu et al. FAU - Xu, Yiwen AU - Xu Y AD - Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. AD - Orthopedics Research Institute, Zhejiang University, Hangzhou, Zhejiang, China. AD - Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang, China. AD - Zhejiang Provincial Clinical Medical Research Center for Motor System Diseases, Hangzhou, Zhejiang, China. AD - International Chinese Musculoskeletal Research Society, Hangzhou, Zhejiang, China. AD - State Key Laboratory of Transvascular Implantation Devices, Hangzhou, Zhejiang, China. FAU - Fang, Miaojie AU - Fang M AD - Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. AD - Orthopedics Research Institute, Zhejiang University, Hangzhou, Zhejiang, China. AD - Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang, China. AD - Zhejiang Provincial Clinical Medical Research Center for Motor System Diseases, Hangzhou, Zhejiang, China. AD - International Chinese Musculoskeletal Research Society, Hangzhou, Zhejiang, China. AD - State Key Laboratory of Transvascular Implantation Devices, Hangzhou, Zhejiang, China. FAU - Chen, Zhenzhong AU - Chen Z AD - Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. AD - Orthopedics Research Institute, Zhejiang University, Hangzhou, Zhejiang, China. AD - Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang, China. AD - Zhejiang Provincial Clinical Medical Research Center for Motor System Diseases, Hangzhou, Zhejiang, China. AD - International Chinese Musculoskeletal Research Society, Hangzhou, Zhejiang, China. AD - State Key Laboratory of Transvascular Implantation Devices, Hangzhou, Zhejiang, China. FAU - Liu, Hong AU - Liu H AD - Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. AD - Orthopedics Research Institute, Zhejiang University, Hangzhou, Zhejiang, China. AD - Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang, China. AD - Zhejiang Provincial Clinical Medical Research Center for Motor System Diseases, Hangzhou, Zhejiang, China. AD - International Chinese Musculoskeletal Research Society, Hangzhou, Zhejiang, China. AD - State Key Laboratory of Transvascular Implantation Devices, Hangzhou, Zhejiang, China. FAU - Xue, Yucheng AU - Xue Y AD - Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. AD - Orthopedics Research Institute, Zhejiang University, Hangzhou, Zhejiang, China. AD - Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang, China. AD - Zhejiang Provincial Clinical Medical Research Center for Motor System Diseases, Hangzhou, Zhejiang, China. AD - International Chinese Musculoskeletal Research Society, Hangzhou, Zhejiang, China. AD - State Key Laboratory of Transvascular Implantation Devices, Hangzhou, Zhejiang, China. FAU - Li, Zilong AU - Li Z AD - Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. AD - Orthopedics Research Institute, Zhejiang University, Hangzhou, Zhejiang, China. AD - Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang, China. AD - Zhejiang Provincial Clinical Medical Research Center for Motor System Diseases, Hangzhou, Zhejiang, China. AD - International Chinese Musculoskeletal Research Society, Hangzhou, Zhejiang, China. AD - State Key Laboratory of Transvascular Implantation Devices, Hangzhou, Zhejiang, China. FAU - Lin, Yue AU - Lin Y AD - Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. AD - Orthopedics Research Institute, Zhejiang University, Hangzhou, Zhejiang, China. AD - Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang, China. AD - Zhejiang Provincial Clinical Medical Research Center for Motor System Diseases, Hangzhou, Zhejiang, China. AD - International Chinese Musculoskeletal Research Society, Hangzhou, Zhejiang, China. AD - State Key Laboratory of Transvascular Implantation Devices, Hangzhou, Zhejiang, China. FAU - Wang, Kelei AU - Wang K AD - Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. AD - Orthopedics Research Institute, Zhejiang University, Hangzhou, Zhejiang, China. AD - Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang, China. AD - Zhejiang Provincial Clinical Medical Research Center for Motor System Diseases, Hangzhou, Zhejiang, China. AD - International Chinese Musculoskeletal Research Society, Hangzhou, Zhejiang, China. AD - State Key Laboratory of Transvascular Implantation Devices, Hangzhou, Zhejiang, China. FAU - Lin, Feng AU - Lin F AD - Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. AD - Orthopedics Research Institute, Zhejiang University, Hangzhou, Zhejiang, China. AD - Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang, China. AD - Zhejiang Provincial Clinical Medical Research Center for Motor System Diseases, Hangzhou, Zhejiang, China. AD - International Chinese Musculoskeletal Research Society, Hangzhou, Zhejiang, China. AD - State Key Laboratory of Transvascular Implantation Devices, Hangzhou, Zhejiang, China. FAU - Zhang, Ning AU - Zhang N AUID- ORCID: 0000-0003-2990-7442 AD - Department of Orthopedic Surgery, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. AD - Orthopedics Research Institute, Zhejiang University, Hangzhou, Zhejiang, China. AD - Key Laboratory of Motor System Disease Research and Precision Therapy of Zhejiang Province, Hangzhou, Zhejiang, China. AD - Zhejiang Provincial Clinical Medical Research Center for Motor System Diseases, Hangzhou, Zhejiang, China. AD - International Chinese Musculoskeletal Research Society, Hangzhou, Zhejiang, China. AD - State Key Laboratory of Transvascular Implantation Devices, Hangzhou, Zhejiang, China. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Research (Wash D C) JT - Research (Washington, D.C.) JID - 101747148 PMC - PMC13710764 COIS- Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/27 CRDT- 2026/10/28 05:02 PHST- 2026/07/31 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:02 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 1459 [pii] AID - 10.34133/research.1459 [doi] PST - epublish SO - Research (Wash D C). 2026 Oct 27;9:1459. doi: 10.34133/research.1459. eCollection 2026. PMID- 42947961 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1662-4548 (Print) IS - 1662-453X (Electronic) IS - 1662-453X (Linking) VI - 20 DP - 2026 TI - Applications of functional ultrasound brain-computer interfaces in neurological diseases: research progress based on neurovascular coupling. PG - 1946753 LID - 10.3389/fnins.2026.1946753 [doi] LID - 1946753 AB - Brain-computer interfaces (BCIs) bypass peripheral nerves and muscles to establish direct information pathways between the brain and external devices, providing novel approaches for motor function reconstruction, communication assistance, and neurorehabilitation in patients with neurological disorders. However, conventional non-invasive BCIs relying on modalities such as electroencephalography (EEG), magnetoencephalography (MEG), or functional magnetic resonance imaging (fMRI) exhibit limitations in spatiotemporal resolution, portability, or real-time capability. Functional ultrasound (fUS), an emerging neuroimaging technology based on neurovascular coupling (NVC), detects cerebral blood volume changes at a spatial resolution of approximately 100 mum; its imaging frame interval is 200-500 ms, whereas its effective temporal resolution, limited by the hemodynamic response, is 1-2 s, offering a potential solution to overcome these constraints. This review comprehensively analyzed NVC-based fUS brain-computer interfaces from the dimensions of technical principles, clinical applications, future directions, and challenges. fUS has demonstrated significant clinical value in stroke assessment, epilepsy monitoring, and early identification of neurodegenerative diseases. Emerging technologies such as transcranial focused ultrasound neuromodulation, sonogenetics, and intravascular ultrasound are driving the evolution of fUS from single-signal "acquisition" toward bidirectional "acquisition-modulation" closed-loop systems, which remain at the proof-of-concept stage. However, the hemodynamic response latency relative to neural electrical activity (1-2 s), the yet-unclear biosafety of chronic ultrasound exposure, and insufficient decoding reliability under pathological conditions remain critical bottlenecks requiring urgent resolution. In conclusion, NVC-based fUS brain-computer interfaces provide a novel tool for precision diagnosis and treatment of neurological diseases. Future efforts should focus on conducting multicenter clinical trials and enhancing the robustness of decoding algorithms. CI - Copyright (c) 2026 Zhang. FAU - Zhang, Chengshan AU - Zhang C AD - The Second Clinical School, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Neurosci JT - Frontiers in neuroscience JID - 101478481 PMC - PMC13710818 OTO - NOTNLM OT - brain-computer interface OT - functional ultrasound imaging OT - neurological diseases OT - neuromodulation OT - neurovascular coupling COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:02 PHST- 2026/07/23 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:02 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fnins.2026.1946753 [doi] PST - epublish SO - Front Neurosci. 2026 Oct 13;20:1946753. doi: 10.3389/fnins.2026.1946753. eCollection 2026. PMID- 42947957 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2045-7758 (Print) IS - 2045-7758 (Electronic) IS - 2045-7758 (Linking) VI - 16 IP - 10 DP - 2026 Oct TI - Multi-Scale Habitat Drivers of Hainan Gibbon Distribution: Integrating Macro-Environmental and Fine-Scale Ecological Factors for Targeted Conservation of Threatened Species. PG - e74519 LID - 10.1002/ece3.74519 [doi] LID - e74519 AB - As habitat fragmentation intensifies due to Anthropocene impacts, ecological variation across spatial scales may cause conservation strategies to misalign with species' actual needs. Understanding how cross-scale spatial heterogeneity in ecological variables shapes species distributions is therefore critical to optimize conservation prioritization. The Critically Endangered Hainan gibbon is restricted to highly degraded forests, yet the interplay of ecological drivers shaping its current distribution, from macro-environmental constraints to fine-scale habitat selection, remains poorly quantified. This study integrates two-year monitoring data from all seven known Hainan gibbon groups (GA-GG, representing the global population) using a multi-scale sampling design. We employed 395 population-level and 135 group-level plots to evaluate 23 ecological variables spanning macro- (e.g., climate) to micro-scales (e.g., food plant diversity), identifying drivers of gibbon distribution. We found that population-scale distribution is driven by climatic and topographic factors (e.g., precipitation, elevation), whereas group-scale distribution responded to fine-scale habitat features (e.g., food plant diversity, terrain ruggedness, crown area). GC distribution reflects optimal foraging (preference for food plant diversity), whereas GE adopts an energy-conserving strategy (selecting complex terrain with high food plant abundance). Habitat suitability followed a J-shaped threshold response at the population scale, and divergent responses at the group level (rapid in GE, stable in GC). These findings underscore the need for scale- and habitat-specific conservation: expanding climatically and vegetatively suitable areas at the population level, restoring food availability in GE habitats, and maintaining stability in GC habitats. Our multi-scale mechanistic framework provides actionable conservation insights, with applications for other range-restricted species in fragmented landscapes. CI - (c) 2026 The Author(s). Ecology and Evolution published by British Ecological Society and John Wiley & Sons Ltd. FAU - Liu, Shuai AU - Liu S AD - School of Ecology - Hainan University Haikou China. AD - The Research and Conservation Centre of Hainan Gibbon Hainan University Haikou China. AD - The National Research and Conservation Centre of Gibbon Bawangling China. AD - School of Ecology and Environment North China University of Water Resources and Electric Power Zhengzhou China. FAU - Zhang, Dexu AU - Zhang D AD - School of Ecology - Hainan University Haikou China. AD - The Research and Conservation Centre of Hainan Gibbon Hainan University Haikou China. AD - The National Research and Conservation Centre of Gibbon Bawangling China. FAU - Cai, Chenglin AU - Cai C AD - The Research and Conservation Centre of Hainan Gibbon Hainan University Haikou China. AD - The National Research and Conservation Centre of Gibbon Bawangling China. AD - School of Tropical Agriculture and Forestry - Hainan University Haikou China. FAU - Zhou, Yidong AU - Zhou Y AD - The Research and Conservation Centre of Hainan Gibbon Hainan University Haikou China. AD - The National Research and Conservation Centre of Gibbon Bawangling China. AD - School of Tropical Agriculture and Forestry - Hainan University Haikou China. FAU - Lu, Kening AU - Lu K AD - The Research and Conservation Centre of Hainan Gibbon Hainan University Haikou China. AD - The National Research and Conservation Centre of Gibbon Bawangling China. AD - School of Tropical Agriculture and Forestry - Hainan University Haikou China. FAU - Chen, Yuan AU - Chen Y AUID- ORCID: 0009-0004-2777-6930 AD - School of Ecology - Hainan University Haikou China. AD - The Research and Conservation Centre of Hainan Gibbon Hainan University Haikou China. AD - The National Research and Conservation Centre of Gibbon Bawangling China. FAU - Long, Wenxing AU - Long W AUID- ORCID: 0000-0002-2709-7442 AD - School of Ecology - Hainan University Haikou China. AD - The Research and Conservation Centre of Hainan Gibbon Hainan University Haikou China. AD - The National Research and Conservation Centre of Gibbon Bawangling China. FAU - Ameca, Eric I AU - Ameca EI AD - School of Ecology - Hainan University Haikou China. AD - The Research and Conservation Centre of Hainan Gibbon Hainan University Haikou China. AD - The National Research and Conservation Centre of Gibbon Bawangling China. AD - IUCN SSC - Primate Specialist Group Gland Switzerland. LA - eng SI - figshare/10.6084/m9.figshare.32676963 PT - Journal Article DEP - 20261026 PL - England TA - Ecol Evol JT - Ecology and evolution JID - 101566408 PMC - PMC13710805 OTO - NOTNLM OT - Nomascus hainanus OT - activity patterns OT - conservation management OT - habitat characteristics OT - population distribution COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/26 CRDT- 2026/10/28 05:02 PHST- 2026/01/10 00:00 [received] PHST- 2026/04/11 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:02 [entrez] PHST- 2026/10/26 00:00 [pmc-release] AID - ECE374519 [pii] AID - 10.1002/ece3.74519 [doi] PST - epublish SO - Ecol Evol. 2026 Oct 26;16(10):e74519. doi: 10.1002/ece3.74519. eCollection 2026 Oct. PMID- 42947952 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2673-5059 (Electronic) IS - 2673-5059 (Linking) VI - 7 DP - 2026 TI - A mobile health-assisted cardiovascular follow-up after pregnancy complications (MumCare): protocol for a randomized controlled trial. PG - 1889953 LID - 10.3389/fgwh.2026.1889953 [doi] LID - 1889953 AB - OBJECTIVE: A history of hypertensive disorders of pregnancy and/or gestational diabetes is associated with increased risk of long-term maternal cardiovascular (CV) disease. Despite recommendations for preventive strategies, few women receive CV follow-up postpartum. We have developed a personalized digital mobile Health companion, the MumCare (Mum's cardiovascular health for life) application, aiming at improving control of modifiable risk factors for CV disease and to bridge hospital and community health services. This paper describes the protocol for a MumCare randomized controlled trial in Norway. METHODS: Study participation is offered to women at Oslo University Hospital with a diagnosis of hypertensive disorders of pregnancy and/or gestational diabetes. Following informed consent (either during pregnancy or within 4 weeks postpartum), a block randomization process assigns participants to one of two groups (access to the Mum Care app or not). The app provides educational material, allows women to track modifiable risk factors, and prompts feedback to registered blood pressures. It also prompts the women to book the nationally recommended follow-up at their general practitioner's at 3-4 and 12 months postpartum. The primary study objective is to assess the effect of MumCare application access on 1-year follow-up rate with a general practitioner. Secondary objectives are to assess group differences at 14 months postpartum regarding: 1) satisfaction with the postpartum mHealth-assisted technology, 2) self-management evaluation, general self-efficacy; and at 14-18 months: 3) modifiable risk factors for CV disease (including hypertension, dyslipidemia, blood sugar control, smoking, weight and physical activity), CV non-invasive hemodynamics and CV-related biomarkers. DISCUSSION: This randomized controlled trial tests the effect of a mobile Health-assisted follow-up of modifiable risk factors after pregnancy complications associated with premature CV disease in women. A study limitation is a risk of underrepresentation of recently immigrated women due to the restricted language options offered in the app. STUDY PROTOCOLS REGISTRATION: NCT05835596. CI - (c) 2026 Staff, Sugulle, Noll, Sollie-Hoel, Oteiza Aguirre, Fiska, Aasbo, Klovning, Estensen and Waldum. FAU - Staff, Anne Cathrine AU - Staff AC AD - Division of Obstetrics and Gynaecology, Oslo University Hospital, Oslo, Norway. AD - Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway. FAU - Sugulle, Meryam AU - Sugulle M AD - Division of Obstetrics and Gynaecology, Oslo University Hospital, Oslo, Norway. AD - Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway. FAU - Noll, Josef AU - Noll J AD - Department of Technology Systems, University of Oslo, Kjeller, Norway. FAU - Sollie-Hoel, Miriam K C AU - Sollie-Hoel MKC AD - Division of Obstetrics and Gynaecology, Oslo University Hospital, Oslo, Norway. AD - Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway. FAU - Oteiza Aguirre, Belen AU - Oteiza Aguirre B AD - Division of Obstetrics and Gynaecology, Oslo University Hospital, Oslo, Norway. AD - Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway. FAU - Fiska, Bendik S AU - Fiska BS AD - Division of Obstetrics and Gynaecology, Oslo University Hospital, Oslo, Norway. AD - Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway. FAU - Aasbo, Gunvor AU - Aasbo G AD - Department of Public Health Science and Interdisciplinary Health Science, Faculty of Medicine, University of Oslo, Oslo, Norway. FAU - Klovning, Atle AU - Klovning A AD - Department of Public Health Science and Interdisciplinary Health Science, Faculty of Medicine, University of Oslo, Oslo, Norway. FAU - Estensen, Mette-Elise AU - Estensen ME AD - ProCardio Centre of Innovation, Department of Cardiology, Rikshospitalet, Oslo University Hospital, Oslo, Norway. AD - Department of Cardiology, Oslo University Hospital, Oslo, Norway. FAU - Waldum, Asa Henning AU - Waldum AH AD - Division of Obstetrics and Gynaecology, Oslo University Hospital, Oslo, Norway. LA - eng SI - ClinicalTrials.gov/NCT05835596 PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Glob Womens Health JT - Frontiers in global women's health JID - 101776281 PMC - PMC13710215 OTO - NOTNLM OT - cardiovascular disease OT - diabetes mellitus OT - empowerment OT - gestational hypertension OT - preeclampsia OT - pregnancy OT - selfcare OT - telemedicine COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 11:36 MHDA- 2026/10/28 11:37 PMCR- 2026/10/13 CRDT- 2026/10/28 05:02 PHST- 2026/05/24 00:00 [received] PHST- 2026/08/06 00:00 [revised] PHST- 2026/09/01 00:00 [accepted] PHST- 2026/10/28 11:37 [medline] PHST- 2026/10/28 11:36 [pubmed] PHST- 2026/10/28 05:02 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fgwh.2026.1889953 [doi] PST - epublish SO - Front Glob Womens Health. 2026 Oct 13;7:1889953. doi: 10.3389/fgwh.2026.1889953. eCollection 2026. PMID- 42947944 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2235-2988 (Electronic) IS - 2235-2988 (Linking) VI - 16 DP - 2026 TI - Integrative analysis reveals gut microbiota-associated host regulatory networks in liver fibrosis. PG - 1963640 LID - 10.3389/fcimb.2026.1963640 [doi] LID - 1963640 AB - BACKGROUND: Gut microbiota-derived metabolites may contribute to liver fibrosis by affecting host inflammatory, metabolic, and epigenetic pathways. We aimed to identify microbiota-associated host genes shared across liver fibrosis of different etiologies. METHODS: Microbiota- and metabolite-associated host genes were obtained from gutMGene v2.0 and integrated with differentially expressed genes from two human liver transcriptomic datasets: GSE84044 for HBV-related fibrosis and GSE49541 for NAFLD-related fibrosis. Shared genes were analyzed using protein-protein interaction and functional enrichment analyses. Selected metabolite-protein interactions were assessed by molecular docking and molecular dynamics simulations. Two-sample Mendelian randomization was used to examine potential associations between candidate gene expression and liver fibrosis risk. RESULTS: Fourteen microbiota-associated genes were differentially expressed in both fibrosis cohorts, including CCL2, CCL20, CXCL6, PTGS2, HDAC2, HDAC3, ADRA2A, SOD1, PEMT, and GNMT. These genes were mainly associated with chemokine activity, histone deacetylation, and IL-17 and TNF signaling. Molecular docking suggested structural compatibility between phenylacetylglutamine and ADRA2A, while butyrate showed weaker predicted interactions with HDAC2 and HDAC3.Molecular dynamics analysis supported the stability of the ADRA2A-phenylacetylglutamine complex under the simulated conditions. Mendelian randomization identified nominal associations of HDAC3, CCL2, PTGS2, and VCL expression with liver fibrosis risk. CONCLUSIONS: This study identified shared microbiota-associated host regulatory candidates in HBV- and NAFLD-related liver fibrosis.HDAC3 and ADRA2A may represent potential links between microbial metabolic signals and fibrotic responses, although further experimental validation is required. CI - Copyright (c) 2026 Peng, Nie, Song, Ding, Cao, Wu, Qi and Zhu. FAU - Peng, Shiyu AU - Peng S AD - Department of Gastroenterology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China. FAU - Nie, Yingying AU - Nie Y AD - Department of Gastroenterology, The First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang, China. FAU - Song, Yuzhu AU - Song Y AD - Department of Infectious Diseases, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. FAU - Ding, Han AU - Ding H AD - Department of Infectious Diseases, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. FAU - Cao, Huiling AU - Cao H AD - Department of Infectious Diseases, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. FAU - Wu, Hao AU - Wu H AD - Department of Infectious Diseases, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. FAU - Qi, Jianni AU - Qi J AD - Department of Central Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. FAU - Zhu, Qiang AU - Zhu Q AD - Department of Gastroenterology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China. AD - Department of Gastroenterology, The First Affiliated Hospital of Shihezi University, Shihezi, Xinjiang, China. AD - Department of Infectious Diseases, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. AD - Department of Central Laboratory, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Cell Infect Microbiol JT - Frontiers in cellular and infection microbiology JID - 101585359 SB - IM MH - Humans MH - *Liver Cirrhosis/genetics/microbiology/metabolism MH - *Gastrointestinal Microbiome MH - *Gene Regulatory Networks MH - Molecular Docking Simulation MH - Molecular Dynamics Simulation MH - Gene Expression Profiling MH - Liver/pathology/metabolism MH - Transcriptome PMC - PMC13710217 OTO - NOTNLM OT - gut microbiota OT - gut-liver axis OT - liver fibrosis OT - microbial metabolites OT - transcriptomics COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 12:34 PMCR- 2026/10/13 CRDT- 2026/10/28 05:01 PHST- 2026/08/10 00:00 [received] PHST- 2026/09/03 00:00 [revised] PHST- 2026/09/07 00:00 [accepted] PHST- 2026/10/28 12:34 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 05:01 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fcimb.2026.1963640 [doi] PST - epublish SO - Front Cell Infect Microbiol. 2026 Oct 13;16:1963640. doi: 10.3389/fcimb.2026.1963640. eCollection 2026. PMID- 42947939 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1663-9812 (Print) IS - 1663-9812 (Electronic) IS - 1663-9812 (Linking) VI - 17 DP - 2026 TI - The cGAS-STING pathway in metabolic dysfunction-associated steatotic liver disease: mechanistic insights and therapeutic opportunities. PG - 1909062 LID - 10.3389/fphar.2026.1909062 [doi] LID - 1909062 AB - Metabolic dysfunction-associated steatotic liver disease (MASLD) is a highly prevalent chronic metabolic liver disease worldwide, encompassing a spectrum that includes simple steatosis, metabolic dysfunction-associated steatohepatitis (MASH), liver fibrosis, and hepatocellular carcinoma (HCC), with immune-metabolic-inflammatory dysregulation at its core pathogenesis. The cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) pathway serves as the central axis of cytosolic DNA sensing and innate immune activation. Endogenous stimuli, including mitochondrial DNA leakage, DNA damage, and gut microbial DNA translocation, can persistently activate this pathway, which in turn regulates lipid metabolism imbalance, hepatic inflammation amplification, hepatic stellate cell (HSC) activation, and malignant transformation through downstream signaling axes, driving the full spectrum of MASLD pathology. Molecules associated with the cGAS-STING pathway may serve as non-invasive diagnostic biomarkers for MASLD, and natural compounds, traditional Chinese medicine, and nanosystems targeting this pathway have demonstrated promising therapeutic effects. This review systematically summarizes the molecular mechanisms of the cGAS-STING pathway, its regulatory roles in MASLD, and advances in targeted therapy, providing a theoretical foundation for disease mechanism elucidation and the development of diagnostic and therapeutic strategies. CI - Copyright (c) 2026 Yuan, Li, Li and Yang. FAU - Yuan, Xingxing AU - Yuan X AD - Department of Gastroenterology, Heilongjiang Academy of Traditional Chinese Medicine, Harbin, China. FAU - Li, Jing AU - Li J AD - Department of Graduate Studies, Academy of Traditional Chinese Medicine, Harbin, China. FAU - Li, Ying AU - Li Y AD - Department of Nephrology, Heilongjiang Academy of Traditional Chinese Medicine, Harbin, China. FAU - Yang, Lei AU - Yang L AD - Department of Gastroenterology, Heilongjiang Academy of Traditional Chinese Medicine, Harbin, China. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Pharmacol JT - Frontiers in pharmacology JID - 101548923 PMC - PMC13710816 OTO - NOTNLM OT - cGAS-STING pathway OT - innate immunity OT - liver fibrosis OT - metabolic dysfunction-associated steatotic liver disease OT - targeted therapy COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/13 CRDT- 2026/10/28 05:01 PHST- 2026/06/14 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 05:01 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 1909062 [pii] AID - 10.3389/fphar.2026.1909062 [doi] PST - epublish SO - Front Pharmacol. 2026 Oct 13;17:1909062. doi: 10.3389/fphar.2026.1909062. eCollection 2026. PMID- 42947937 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-2295 (Electronic) IS - 1664-2295 (Linking) VI - 17 DP - 2026 TI - Accuracy of CSF tap test and lumbar infusion test in predicting shunt response in idiopathic normal pressure hydrocephalus: a systematic review and meta-analysis. PG - 1908328 LID - 10.3389/fneur.2026.1908328 [doi] LID - 1908328 AB - BACKGROUND: Idiopathic normal pressure hydrocephalus (iNPH) presents with gait disturbance, cognitive impairment, and urinary incontinence. The cerebrospinal fluid tap test (CSF-TT) and lumbar infusion test (LIT) are commonly used to predict postoperative improvement after shunt surgery; however, their validity remains debated. METHODS: We performed a systematic review and meta-analysis to assess the sensitivity and specificity of CSF-TT and LIT. The protocol was registered in PROSPERO (CRD42023454502). Reporting followed the PRISMA-DTA guidelines, and the certainty of evidence was assessed using the GRADE approach. RESULTS: Of 1,955 studies, 15 were included, reporting on 721 CSF-TT and 393 LIT patients, with shunt surgery as the reference standard. Nine CSF-TT studies (n = 700) and five LIT studies (n = 368) contributed to the primary meta-analysis. Considerable heterogeneity existed in test protocols, timing of assessment, and outcome measures. Several studies were retrospective with a high risk of bias. The pooled estimates were derived from a limited number of heterogeneous studies. Using a bivariate random-effects model, pooled sensitivity and specificity of CSF-TT were 64.1% (95% CI 49.2-76.6%, I (2) 81.3%) and 54.8% (95% CI 41.8-67.2%, I (2) 59.7%), respectively. The certainty of evidence was very low for sensitivity due to bias, inconsistency, and imprecision, and low for poor specificity due to bias and inconsistency. LIT showed a pooled sensitivity of 77.5% (95% CI 70.2-83.4%, I (2) 14.7%) and a specificity of 28.3% (95% CI 19.0-39.9%, I (2) 4.6%), with moderate certainty for sensitivity and moderate certainty for poor specificity. CONCLUSION: Both CSF-TT and LIT show poor specificity for predicting shunt responsiveness in iNPH, with low-certainty evidence for CSF-TT and moderate-certainty evidence for LIT. A positive result from either test should not be used in isolation to guide surgical decisions. Standardized prospective studies with clearly defined patient selection criteria and outcome measures are needed to establish the true diagnostic value of these tests. SYSTEMATIC REVIEW REGISTRATION: https://www.crd.york.ac.uk/PROSPERO/view/CRD42023454502. CI - Copyright (c) 2026 Aliyar, Dash, Fasano, Prasad, Dhikav, Wagle Shukla, Upadhyay, Desai, Poudel, Pal and Elavarasi. FAU - Aliyar, Aminu AU - Aliyar A AD - Department of Neurology, All India Institute of Medical Sciences, New Delhi, India. FAU - Dash, Deepa AU - Dash D AD - Department of Clinical Neurologic Sciences, University of Western Ontario, London, ON, Canada. FAU - Fasano, Alfonso AU - Fasano A AD - Department of Biomedical Sciences, Humanitas University, Pieve Emanuele, Milan, Italy. AD - IRCCS Humanitas Research Hospital, Rozzano, Milan, Italy. FAU - Prasad, Manya AU - Prasad M AD - Center for Community Medicine, All India Institute of Medical Sciences, New Delhi, India. FAU - Dhikav, Vikas AU - Dhikav V AD - Center for Evidence for Guidelines, Department of Health Research, MoHFW, Govt of India, New Delhi, India. FAU - Wagle Shukla, Aparna AU - Wagle Shukla A AD - Department of Neurology, Fixel Institute for Neurological Diseases, University of Florida, Gainesville, FL, United States. FAU - Upadhyay, Ashish Datt AU - Upadhyay AD AD - Clinical Research Unit, All India Institute of Medical Sciences, New Delhi, India. FAU - Desai, Soaham AU - Desai S AD - Department of Neurology, Pramukhswami Medical College, Karamsad, Anand, India. FAU - Poudel, Sagar AU - Poudel S AD - Department of Medicine, All India Institute of Medical Sciences, New Delhi, India. FAU - Pal, Pramod Kumar AU - Pal PK AD - Department of Neurology, National Institute of Mental Health and Neurosciences, Bengaluru, India. FAU - Elavarasi, Arunmozhimaran AU - Elavarasi A AD - Department of Neurology, All India Institute of Medical Sciences, New Delhi, India. LA - eng PT - Journal Article PT - Meta-Analysis PT - Systematic Review DEP - 20261012 PL - Switzerland TA - Front Neurol JT - Frontiers in neurology JID - 101546899 SB - IM MH - Humans MH - *Hydrocephalus, Normal Pressure/surgery/diagnosis/cerebrospinal fluid MH - *Spinal Puncture/standards/methods MH - *Cerebrospinal Fluid Shunts MH - Sensitivity and Specificity MH - Infusions, Spinal PMC - PMC13710815 OTO - NOTNLM OT - CSF tap test OT - NPH OT - diagnostic accuracy OT - lumbar infusion test OT - normal pressure hydrocephalus OT - sensitivity OT - specificity COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 12:34 PMCR- 2026/10/12 CRDT- 2026/10/28 05:01 PHST- 2026/06/13 00:00 [received] PHST- 2026/07/22 00:00 [revised] PHST- 2026/07/31 00:00 [accepted] PHST- 2026/10/28 12:34 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 05:01 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - 10.3389/fneur.2026.1908328 [doi] PST - epublish SO - Front Neurol. 2026 Oct 12;17:1908328. doi: 10.3389/fneur.2026.1908328. eCollection 2026. PMID- 42947934 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1179-1314 (Print) IS - 1179-1314 (Electronic) IS - 1179-1314 (Linking) VI - 18 DP - 2026 TI - Predictive Value of Combined Ultrasound Viscoelasticity Imaging, CEUS, AAPR, and PLR for Assessing Neoadjuvant Chemotherapy Efficacy in Breast Cancer. PG - 636920 LID - 10.2147/BCTT.S636920 [doi] LID - 636920 AB - BACKGROUND: The present study addresses the clinical demand for reliable prediction of neoadjuvant chemotherapy (NAC) efficacy in breast cancer and seeks to overcome the limitations of conventional approaches (such as imaging and pathology), including limited sensitivity, invasiveness, and delayed assessment. The clinical utility of viscoelasticity imaging, contrast-enhanced ultrasound (CEUS), and peripheral blood inflammatory markers (AAPR, PLR) for early and accurate prediction of NAC efficacy was evaluated using a multimodal strategy. METHODS: A retrospective cohort study was conducted, including 105 patients with locally advanced breast cancer. According to Miller-Payne pathological grading, patients were classified into a major histologically response group (MHR) and a non-major histologically response group (NMHR). Prior to NAC, viscoelastic parameters (eg, Emax, Vmax) and CEUS parameters (e.g. PI, TTP, MTT) were obtained by ultrasound examination, and peripheral blood inflammatory markers were collected. Predictive performance was analyzed using multivariate logistic regression and ROC curve analysis. RESULTS: A higher proportion of HER-2 positivity was observed in the MHR group (P<0.05). Compared with the NMHR group, Emax, Vmax, and PI values were reduced, whereas TTP and MTT were increased (P<0.001). In addition, peripheral blood PLR levels were lower and AAPR values were higher in the MHR group (P<0.001). Multivariate analysis identified these variables as independent influencing factors (OR range 0.288-8.232). ROC curve analysis demonstrated that the multimodal model achieved an AUC of 0.945, with a sensitivity of 89.71% and a specificity of 86.49%, outperforming individual predictive methods. CONCLUSION: Integrated assessment using viscoelastic imaging, CEUS, and peripheral blood biomarkers showed potential for stratifying NAC response. Further validation in larger, multicenter cohorts is needed to confirm its generalizability and clinical utility. CI - (c) 2026 Li and Shi. FAU - Li, Meina AU - Li M AD - Department of Ultrasound, Taizhou People's Hospital, Taizhou, Jiangsu, 225300, People's Republic of China. FAU - Shi, Jie AU - Shi J AD - Department of Ultrasound, Taizhou Hospital of Traditional Chinese Medicine, Taizhou, Jiangsu, 225300, People's Republic of China. LA - eng PT - Journal Article DEP - 20261022 PL - New Zealand TA - Breast Cancer (Dove Med Press) JT - Breast cancer (Dove Medical Press) JID - 101591856 PMC - PMC13710117 OTO - NOTNLM OT - Breast cancer OT - NAC OT - contrast-enhanced ultrasound OT - exploration of viscoelasticity imaging technique OT - peripheral blood inflammatory markers COIS- The authors report no conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/22 CRDT- 2026/10/28 05:01 PHST- 2026/06/30 00:00 [received] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 05:01 [entrez] PHST- 2026/10/22 00:00 [pmc-release] AID - 636920 [pii] AID - 10.2147/BCTT.S636920 [doi] PST - epublish SO - Breast Cancer (Dove Med Press). 2026 Oct 22;18:636920. doi: 10.2147/BCTT.S636920. eCollection 2026. PMID- 42947929 OWN - NLM STAT- Publisher LR - 20261029 IS - 0024-3639 (Print) IS - 2050-8549 (Electronic) IS - 0024-3639 (Linking) DP - 2026 Oct 26 TI - Editorial Integrity in Medical Journals: A Conceptual Review of Moral Foundations for Publication Ethics. PG - 00243639261496957 LID - 10.1177/00243639261496957 [doi] LID - 00243639261496957 AB - Publication ethics in medicine has developed extensive procedural guidance concerning authorship, peer review, conflicts of interest, editorial independence, and correction of the scientific record. Less attention has been devoted to the moral goods that these norms are intended to protect. This article examines whether selected categories drawn from Catholic moral thought and Catholic social teaching can illuminate the ethical foundations of biomedical editorial practice without replacing established international standards. Using a conceptual and interpretive approach, the article examines the responsibilities of authors, reviewers, editors, publishers, and research institutions through the lenses of truthfulness, justice, prudence, non-maleficence, and the common good. The discussion focuses especially on plagiarism, retraction, and editorial independence as areas in which contemporary publication ethics and Catholic moral reflection converge. The analysis suggests that biomedical editorial ethics and Catholic moral thought address the same questions from distinct yet complementary perspectives. Procedural standards identify duties and corrective mechanisms, whereas moral reflection clarifies the human goods that these practices are intended to protect. Under this interpretation, editorial integrity emerges not merely as regulatory compliance but as an institutional responsibility directed toward the reliability of biomedical knowledge, the protection of patients and readers, and the preservation of public trust. Read as a conceptual contribution rather than a confessional proposal, this framework offers operational criteria for evaluating editorial conduct and clarifies why the integrity of the scientific record matters for clinical care, public health, and social trust. CI - (c) Catholic Medical Association 2026. FAU - Pardal-Refoyo, Jose Luis AU - Pardal-Refoyo JL AUID- ORCID: 0000-0002-7462-1606 AD - Revista ORL (Salamanca University Editions), University of Salamanca, Salamanca, Spain. AD - Department of Surgery, Otorhinolaryngology Area, University Hospital of Salamanca, Salamanca, Spain. AD - Otorhinolaryngology and Head and Neck Surgery Service, IBSAL (Institute of Biomedical Research of Salamanca), Salamanca, Spain. FAU - Pardal-Pelaez, Beatriz AU - Pardal-Pelaez B AUID- ORCID: 0000-0001-5608-7924 AD - Department of Surgery, Dentistry Area, Faculty of Medicine and Dental Clinic, University of Salamanca, Salamanca, Spain. RINGGOLD: 83168 LA - eng PT - Editorial DEP - 20261026 PL - United States TA - Linacre Q JT - The Linacre quarterly JID - 2985221R PMC - PMC13710432 OTO - NOTNLM OT - Publication ethics OT - bioethics OT - editorial ethics OT - medical journals OT - narrative review OT - research integrity COIS- The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 PMCR- 2027/10/26 CRDT- 2026/10/28 05:01 PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 05:01 [entrez] PHST- 2027/10/26 00:00 [pmc-release] AID - 10.1177_00243639261496957 [pii] AID - 10.1177/00243639261496957 [doi] PST - aheadofprint SO - Linacre Q. 2026 Oct 26:00243639261496957. doi: 10.1177/00243639261496957. PMID- 42947925 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1976-8257 (Print) IS - 2234-2753 (Electronic) IS - 1976-8257 (Linking) VI - 42 IP - 6 DP - 2026 Nov TI - Risk assessment of benzophenone-3, a UV filter in cosmetics. PG - 705-724 LID - 10.1007/s43188-026-00364-w [doi] AB - Benzophenone-3 (also known as oxybenzone) is an ultraviolet filter commonly used in sunscreen formulations and personal care products. Given its systemic exposure through dermal and oral absorption, the toxicity of benzophenone-3 has been widely studied. In rodents, dietary exposure to high experimental doses of benzophenone-3 was associated with mild systemic toxicity, including reduction in body weight gain, increase in liver and kidney weights, and signs of nephropathy. While benzophenone-3 was shown to be non-irritating and non-skin sensitizing following ocular instillation and dermal application in animal studies, it has been recognized as a cause of photoallergic contact dermatitis in clinical studies. Mild reproductive and developmental effects have also been reported, including reduced offspring body weight, slightly reduced litter size, and altered sperm parameters. Benzophenone-3 was non-mutagenic in genotoxicity assays, and a lifetime dietary exposure study in rodents suggested equivocal evidence of carcinogenic activity. The endocrine-disrupting potential of benzophenone-3 is of concern because it binds to estrogen receptors. Although in vitro and in vivo assays have shown inconsistent results, the potential risk to humans remains a concern. For the safety assessment, a no-observed-adverse-effect level of 1,000 ppm (67.9 mg/kg/day) was selected from a reproductive and developmental toxicity study in which a reduced number of spermatocytes per seminiferous tubule was observed in male F1 offspring. The systemic exposure dose, calculated based on the daily usage amount for the Korean population, was 0.6732 mg/kg/day when used at 2.4% in sunscreen products, resulting in a margin of safety for benzophenone-3 that was approximately equal to or greater than 100. Therefore, the use of benzophenone-3 in cosmetics under current regulations is considered safe. CI - (c) The Author(s) under exclusive licence to Korean Society of Toxicology 2026. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. FAU - Kang, Kyu-Tae AU - Kang KT AD - College of Pharmacy, Duksung Women's University, Seoul, 01369 Republic of Korea. ROR: https://ror.org/01h6frr69. GRID: grid.410884.1. ISNI: 0000 0004 0532 6173 FAU - Kim, Minhyuk AU - Kim M AD - College of Pharmacy, The Catholic University of Korea, Bucheon, 14662 Republic of Korea. ROR: https://ror.org/01fpnj063. GRID: grid.411947.e. ISNI: 0000 0004 0470 4224 FAU - Sung, Chi Rim AU - Sung CR AD - School of Advanced Biosciences, GAST, Changwon National University, Changwon, 51140 Republic of Korea. ROR: https://ror.org/04ts4qa58. GRID: grid.411214.3. ISNI: 0000 0001 0442 1951 FAU - Lee, Jung Dae AU - Lee JD AD - College of Pharmacy and Center for Human Risk Assessment, Dankook University, Cheonan, 31116 Republic of Korea. ROR: https://ror.org/058pdbn81. GRID: grid.411982.7. ISNI: 0000 0001 0705 4288 FAU - Yoon, Taehyung AU - Yoon T AD - Cosmetic Research Division, National Institute of Food and Drug Safety Evaluation, Cheongju, 28159 Republic of Korea. ROR: https://ror.org/027k9sa32. GRID: grid.467691.b. ISNI: 0000 0004 1773 0675 FAU - Kim, Kyu-Bong AU - Kim KB AD - College of Pharmacy and Center for Human Risk Assessment, Dankook University, Cheonan, 31116 Republic of Korea. ROR: https://ror.org/058pdbn81. GRID: grid.411982.7. ISNI: 0000 0001 0705 4288 FAU - Kwack, Seung Jun AU - Kwack SJ AD - School of Advanced Biosciences, GAST, Changwon National University, Changwon, 51140 Republic of Korea. ROR: https://ror.org/04ts4qa58. GRID: grid.411214.3. ISNI: 0000 0001 0442 1951 FAU - Lee, Joo Young AU - Lee JY AUID- ORCID: 0000-0002-6020-3040 AD - College of Pharmacy, The Catholic University of Korea, Bucheon, 14662 Republic of Korea. ROR: https://ror.org/01fpnj063. GRID: grid.411947.e. ISNI: 0000 0004 0470 4224 LA - eng PT - Journal Article PT - Review DEP - 20260610 PL - Singapore TA - Toxicol Res JT - Toxicological research JID - 101483324 PMC - PMC13710491 OTO - NOTNLM OT - Cosmetics OT - Endocrine disruption OT - Risk assessment OT - Toxicity OT - UV filter COIS- Conflict of interestJoo Young Lee is an Associate Editor of this journal Toxicological Research, but this position had no influence on the editorial decision or the peer review process of this manuscript. All other authors declare that they have no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2027/11/01 CRDT- 2026/10/28 05:00 PHST- 2025/12/14 00:00 [received] PHST- 2026/03/31 00:00 [revised] PHST- 2026/05/08 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 05:00 [entrez] PHST- 2027/11/01 00:00 [pmc-release] AID - 364 [pii] AID - 10.1007/s43188-026-00364-w [doi] PST - epublish SO - Toxicol Res. 2026 Jun 10;42(6):705-724. doi: 10.1007/s43188-026-00364-w. eCollection 2026 Nov. PMID- 42947908 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1179-1314 (Print) IS - 1179-1314 (Electronic) IS - 1179-1314 (Linking) VI - 18 DP - 2026 TI - Glucose-Dependent Cytotoxic Responses in Triple-Negative Breast Cancer: Experimental and Transcriptomic Evidence of Metabolic Context and p38 MAPK-Associated Modulation. PG - 623153 LID - 10.2147/BCTT.S623153 [doi] LID - 623153 AB - BACKGROUND: Triple-negative breast cancer exhibits substantial metabolic heterogeneity, which may influence therapeutic response. This study investigated whether thymoquinone, glabridin, and glycyrrhizic acid exhibit context-dependent cytotoxicity under glucose deprivation and hypoxia, and whether pharmacological inhibition of p38 MAPK modifies these responses. METHODS: MDA-MB-231 and MCF-7 cells were treated in 2D monolayer culture with thymoquinone, glabridin, glycyrrhizic acid, or doxorubicin under normoxic, glucose-deprived, and hypoxic conditions. Cell viability was assessed by MTT assay, and p38 involvement was explored using SB203580 co-treatment. Selectivity was assessed in MCF-10A cells. Thymoquinone silver-nanoparticles were also evaluated. Bulk TCGA-BRCA basal-like and receptor-defined TNBC cohorts and GSE118389 single-cell TNBC RNA-seq data were analyzed for glycolysis, hypoxia, p38 MAPK, epithelial-mesenchymal transition, stemness, and oxidative-stress signatures. RESULTS: In MDA-MB-231 cells, glabridin showed greater cytotoxicity under glucose-replete conditions, whereas glycyrrhizic acid became more cytotoxic during glucose deprivation. The effect of SB203580 varied according to metabolic context, supporting pharmacologically inferred rather than directly validated p38 involvement. Doxorubicin activity was reduced under hypoxia, whereas thymoquinone retained cytotoxic activity. Thymoquinone nanoparticles did not demonstrate clear therapeutic selectivity. Transcriptomic analyses identified a glycolysis-hypoxia relationship. After false-discovery-rate correction, higher p38-related transcriptional activity was associated with increased EMT scores in both bulk TNBC cohorts. Hallmark sensitivity analyses additionally showed higher glycolysis, hypoxia, and EMT scores in p38-high tumors. Single-cell analyses identified similar metabolic-stress and adaptive associations but were exploratory because cells were derived from only six tumors. CONCLUSION: Metabolic context influenced cytotoxic responses in TNBC. These findings support further investigation of microenvironment-adapted therapies, while p38-related mechanisms require direct validation. CI - (c) 2026 Rahman et al. FAU - Rahman, Ishrat AU - Rahman I AUID- ORCID: 0000-0002-6530-1504 AD - Department of Basic Dental Sciences, College of Dentistry, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. FAU - Henidi, Hanan AU - Henidi H AD - Research Department, Natural and Health Sciences Research Center, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. FAU - Alshibli, Aisha AU - Alshibli A AD - College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. FAU - Bin-Murdhi, Nouf Saleh AU - Bin-Murdhi NS AD - Research Department, Natural and Health Sciences Research Center, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. FAU - Alqutaym, Maram Saud AU - Alqutaym MS AD - College of Pharmacy, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. FAU - Alsheddi, Manal AU - Alsheddi M AD - Department of Basic Dental Sciences, College of Dentistry, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. FAU - Alghamdi, Sahar AU - Alghamdi S AUID- ORCID: 0000-0002-2770-218X AD - Department of Pharmaceutical Sciences, College of Pharmacy, King Saud bin Abdulaziz University for Health Sciences (KSAU-HS), Riyadh, Saudi Arabia. AD - King Abdullah International Medical Research Center (KAIMRC), Riyadh, Saudi Arabia. AD - King Abdulaziz Medical City, National Guard Health Affairs (NGHA), Riyadh, Saudi Arabia. FAU - Bin Rayyis Snr, Aljawharah F AU - Bin Rayyis Snr AF AD - College of Pharmacy, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. FAU - Alqahtani, Reem Abdullah AU - Alqahtani RA AD - College of Pharmacy, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. FAU - Aljaddua, Lubna Khalid AU - Aljaddua LK AUID- ORCID: 0009-0009-5622-2060 AD - College of Pharmacy, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. FAU - Mohammed, Afrah E AU - Mohammed AE AD - Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. AD - Microbiology and Immunology Unit, Natural and Health Sciences Research Center, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261022 PL - New Zealand TA - Breast Cancer (Dove Med Press) JT - Breast cancer (Dove Medical Press) JID - 101591856 PMC - PMC13710119 OTO - NOTNLM OT - glabridin OT - glycyrrhizic acid OT - hypoxia OT - metabolic stress OT - p38 MAPK OT - single-cell RNA sequencing OT - thymoquinone OT - tumor microenvironment COIS- All authors declare that they have no competing interests. Generative AI and AI-assisted tools were used to support language refinement, manuscript editing, figure concept development, and schematic visual drafting. M365 Copilot (Basic) and Grammarly 1.164.0.0 were used for language refinement and editing. ChatGPT (OpenAI; GPT-5.6 Sol) was used to assist with the preparation and refinement of schematic illustrations, including the computational workflow figure, conceptual model, and graphical abstract. The authors reviewed and verified all AI-assisted content and confirm the originality and accuracy of the final material. The authors reviewed the applicable terms of use for the AI tools employed and confirm their suitability for publication. The authors confirm that they have the right to publish the AI-assisted images and that any required permissions have been obtained. All relevant image versions and records of prompts have been retained and can be provided upon request. The authors take full responsibility for the integrity and accuracy of the entire manuscript, including all figures, scientific content, and references. AI tools were not used to generate experimental data, perform laboratory experiments, conduct statistical analyses, or replace the authors' scientific interpretation. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/22 CRDT- 2026/10/28 04:59 PHST- 2026/05/30 00:00 [received] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:59 [entrez] PHST- 2026/10/22 00:00 [pmc-release] AID - 623153 [pii] AID - 10.2147/BCTT.S623153 [doi] PST - epublish SO - Breast Cancer (Dove Med Press). 2026 Oct 22;18:623153. doi: 10.2147/BCTT.S623153. eCollection 2026. PMID- 42947900 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1205-7088 (Print) IS - 1918-1485 (Electronic) IS - 1205-7088 (Linking) VI - 31 IP - 7 DP - 2026 Nov TI - La premedication en vue de la laryngoscopie chez les nouveau-nes. PG - 809-817 LID - 10.1093/pch/pxag041 [doi] AB - La laryngoscopie, courante en soins intensifs neonatals, a des effets indesirables confirmes. Il est demontre que la premedication reduit ces effets et accroit le taux de reussite. Dans toutes les intubations non urgentes, il faut administrer une premedication composee d'un vagolytique, d'un opioide a action rapide et d'un relaxant musculaire a courte duree d'action. Des mesures non pharmacologiques sont toutefois toujours a privilegier en cas d'administration non invasive de surfactant. Les opioides a action rapide sont egalement recommandes, en combinaison avec un vagolytique. La laryngoscopie doit toujours etre executee ou supervisee par un prestataire de soins qualifie, conjuguee a une surveillance cardiorespiratoire continue. CI - (c) Societe canadienne de pediatrie 2026. Publie par Oxford University Press pour le compte de la Societe canadienne de pediatrie. Tous droits reserves. Pour en faire une reutilisation commerciale, veuillez ecrire a reprints@oup.com afin d'obtenir les droits de reimpression et de traduction. Toutes les autres autorisations peuvent etre obtenues aupres du service RightsLink accessible par le lien Permissions sur la page de l'article affichee dans notre site. Pour obtenir plus d'information, ecrivez a journals.permissions@oup.com. FAU - Gervais, Anne-Sophie AU - Gervais AS AD - Societe canadienne de pediatrie, comite d'etude du foetus et du nouveau-ne, Ottawa (Ontario) Canada. FAU - Barrington, Keith J AU - Barrington KJ AD - Societe canadienne de pediatrie, comite d'etude du foetus et du nouveau-ne, Ottawa (Ontario) Canada. FAU - Finan, Emer AU - Finan E AD - Societe canadienne de pediatrie, comite d'etude du foetus et du nouveau-ne, Ottawa (Ontario) Canada. LA - eng PT - Journal Article PT - Review DEP - 20260707 PL - England TA - Paediatr Child Health JT - Paediatrics & child health JID - 9815960 PMC - PMC13710193 OTO - NOTNLM OT - administration de surfactant OT - administration moins invasive de surfactant OT - laryngoscopie OT - nouveau-ne OT - premedication OT - traitement mini-invasif au surfactant COIS- Conflits d'interets potentiels Les auteurs n'ont aucuns conflits d'interets a declarer. Les auteurs ont remis le formulaire de divulgation des conflits d'interets potentiels de l'International Committee of Medical Journal Editors. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2027/07/07 CRDT- 2026/10/28 04:59 PHST- 2026/04/22 00:00 [received] PHST- 2026/04/23 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:59 [entrez] PHST- 2027/07/07 00:00 [pmc-release] AID - pxag041 [pii] AID - 10.1093/pch/pxag041 [doi] PST - epublish SO - Paediatr Child Health. 2026 Jul 7;31(7):809-817. doi: 10.1093/pch/pxag041. eCollection 2026 Nov. PMID- 42947897 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-2565 (Electronic) IS - 2296-2565 (Linking) VI - 14 DP - 2026 TI - Expansion of Korea's Special Estimate Case System and changes in out-of-pocket expenditure: an interrupted time-series analysis. PG - 1927340 LID - 10.3389/fpubh.2026.1927340 [doi] LID - 1927340 AB - BACKGROUND: Since the 2000s, Korea has expanded its National Health Insurance coverage through the implementation and expansion of the Special Estimate Case System. This study examined changes in the proportion of out-of-pocket (OOP) expenditure associated with these policy changes. METHODS: We analyzed nationwide National Health Insurance Database data from 2002 to 2024. Interrupted time-series (ITS) analysis using monthly data was performed to examine changes in the proportion of OOP expenditure relative to total medical expenditure associated with the implementation (September 2005) and expansion (July 2009) of the Special Estimate Case System. An additional controlled interrupted time-series (CITS) analysis compared changes between care covered and not covered under the system following the 2009 expansion. RESULTS: The proportion of OOP expenditure decreased from 27.1% in 2005 to 25.3% in 2006 and from 25.4% in 2009 to 24.3% in 2010. The ITS analysis showed immediate reductions of 1.78 percentage points following implementation and 0.47 percentage points following expansion for total care. In the additional CITS analysis, the differential reduction following the 2009 expansion was 3.11 percentage points for total care, indicating a greater reduction in the OOP expenditure proportion for care covered under the system than for care not covered under the system. CONCLUSION: The implementation and expansion of the Special Estimate Case System were associated with reductions in the proportion of OOP expenditure, although the patterns differed by type of care and healthcare institution. After excluding medical expenditure for care covered under the system, OOP expenditure proportions were closer to the statutory copayment rates under the NHI. These findings may inform future policy discussions on the selection of eligible diseases and patient groups and the setting of copayment rates under the Special Estimate Case System. CI - Copyright (c) 2026 Bae, Kim and Park. FAU - Bae, Suk Won AU - Bae SW AD - Health Insurance Research Institute, National Health Insurance Service, Wonju, Republic of Korea. FAU - Kim, Jeongha AU - Kim J AD - Health Insurance Research Institute, National Health Insurance Service, Wonju, Republic of Korea. FAU - Park, Wha Me AU - Park WM AD - The Institute for Occupational Health, Yonsei University College of Medicine, Seoul, Republic of Korea. AD - Graduate School of Public Health, Yonsei University, Seoul, Republic of Korea. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Public Health JT - Frontiers in public health JID - 101616579 SB - IM MH - Republic of Korea MH - Interrupted Time Series Analysis MH - *Health Expenditures/statistics & numerical data/trends MH - Humans MH - *National Health Programs/economics/statistics & numerical data MH - *Financing, Personal/statistics & numerical data/economics PMC - PMC13710395 OTO - NOTNLM OT - National Health Insurance OT - National Health Insurance Database OT - Special Estimate Case OT - interrupted time series OT - out-of-pocket expenditure COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 12:34 PMCR- 2026/10/13 CRDT- 2026/10/28 04:59 PHST- 2026/07/03 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/28 12:34 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:59 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fpubh.2026.1927340 [doi] PST - epublish SO - Front Public Health. 2026 Oct 13;14:1927340. doi: 10.3389/fpubh.2026.1927340. eCollection 2026. PMID- 42947896 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2331-8325 (Electronic) IS - 2331-8325 (Linking) VI - 16 IP - 20 DP - 2026 Oct 20 TI - Quantitative Colocalization Analysis in Fluorescence Microscopy. PG - e5832 LID - 10.21769/BioProtoc.5832 [doi] LID - e5832 AB - Spatial organization of macromolecules is fundamental to cellular function, with colocalization providing key insights into molecular interactions and biological processes. However, quantification remains challenging due to diverse localization patterns and irregular sample geometries. Here, we present a protocol for analyzing colocalization between two fluorescent probes using coAnalyzer, a MATLAB-based software package. coAnalyzer features a user-friendly graphical interface and supports region of interest (ROI) selection, image merging, line scanning, signal isolation, scatterplot generation, and quantitative analysis. coAnalyzer enables colocalization analysis of any two fluorophores, regardless of the proteins or dyes involved. Its broad applicability across a wide range of organisms and sample types demonstrates the robustness, flexibility, and versatility of the platform. Key features * Provides both quantitative and semi-quantitative colocalization analysis using multiple coefficients. * Features a user-friendly graphical user interface (GUI) that provides step-by-step visualization of the analysis process. * Supports 1D (line scan) and 2D analysis. * Analyzes large datasets and generates histograms for effective data presentation. CI - (c)Copyright : (c) 2026 The Authors; This is an open access article under the CC BY license. FAU - Myers, Charles James AU - Myers CJ AD - Department of Biology, Texas A&M University, College Station, TX, USA. FAU - Nan, Beiyan AU - Nan B AD - Department of Biology, Texas A&M University, College Station, TX, USA. LA - eng PT - Journal Article DEP - 20261020 PL - United States TA - Bio Protoc JT - Bio-protocol JID - 101635102 PMC - PMC13710125 OTO - NOTNLM OT - Colocalization OT - Fluorescence microscopy OT - Manders' correlation OT - Pearson's correlation OT - Quantification COIS- Competing interestsThe authors declare no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/20 CRDT- 2026/10/28 04:59 PHST- 2026/05/28 00:00 [received] PHST- 2026/08/31 00:00 [revised] PHST- 2026/09/01 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:59 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - e5832 [pii] AID - 5832 [pii] AID - 10.21769/BioProtoc.5832 [doi] PST - epublish SO - Bio Protoc. 2026 Oct 20;16(20):e5832. doi: 10.21769/BioProtoc.5832. eCollection 2026 Oct 20. PMID- 42947894 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-634X (Print) IS - 2296-634X (Electronic) IS - 2296-634X (Linking) VI - 14 DP - 2026 TI - Pancreatic acinar cell carcinoma demonstrates marked clinical heterogeneity and recurrent actionable genomic alterations. PG - 1913398 LID - 10.3389/fcell.2026.1913398 [doi] LID - 1913398 AB - BACKGROUND: Pancreatic acinar cell carcinoma (PACC) is a rare pancreatic malignancy with a molecular profile distinct from pancreatic ductal adenocarcinoma (PDAC) but is commonly treated using extrapolated paradigms. METHODS: We retrospectively identified patients with histologically confirmed PACC at a tertiary academic center and abstracted clinical, pathologic, treatment, and outcome data. Next-generation sequencing (NGS) was reviewed for microsatellite status, tumor mutational burden, and actionable genomic alterations. RESULTS: Seven patients were identified (median age, 64 years; 57% male). Most presented with metastatic disease (71%), yet outcomes were heterogeneous, including prolonged progression-free survival exceeding 7 years and 16 years in select patients. Carbohydrate antigen 19-9 (CA 19-9) was normal in all cases, and jaundice was absent despite frequent pancreatic head involvement. All tumors were KRAS wild-type. NGS revealed recurrent alterations including CDKN2A/B and MTAP co-deletion, BRAF fusion, IKZF1 loss, and BAP1 mutation. Tumors were microsatellite stable with low-to-intermediate tumor mutational burden. One patient received matched targeted therapy through a precision oncology trial. CONCLUSION: PACC demonstrates substantial clinical heterogeneity with a subset of patients exhibiting prolonged survival despite metastatic disease. Recurrent actionable genomic alterations support routine molecular profiling and prioritization for biomarker-driven trials. These findings challenge extrapolation from PDAC and support a precision oncology framework for this ultra-rare malignancy. CI - Copyright (c) 2026 Farhoud, Al-Rajabi, Baranda, Li, Hamza, Zhang, Paluri, Manne, Dandawate, Saha, Schmitt, Kumer, King, Olyaee, Rastogi, Hejazi, Sun and Kasi. FAU - Farhoud, Noor AU - Farhoud N AD - Department of Internal Medicine, University of Kansas Medical Center, Kansas City, KS, United States. FAU - Al-Rajabi, Raed AU - Al-Rajabi R AD - Department of Internal Medicine, Division of Medical Oncology, University of Kansas Medical Center, Westwood, KS, United States. FAU - Baranda, Joaquina AU - Baranda J AD - Department of Internal Medicine, Division of Medical Oncology, University of Kansas Medical Center, Westwood, KS, United States. FAU - Li, Haoran AU - Li H AD - Department of Internal Medicine, Division of Medical Oncology, University of Kansas Medical Center, Westwood, KS, United States. FAU - Hamza, Ameer AU - Hamza A AD - Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, United States. FAU - Zhang, Wei AU - Zhang W AD - Department of Internal Medicine, Division of Medical Oncology, University of Kansas Medical Center, Westwood, KS, United States. FAU - Paluri, Ravi AU - Paluri R AD - Department of Hematology and Oncology, Wake Forest School of Medicine, Winston-Salem, NC, United States. FAU - Manne, Ashish AU - Manne A AD - Department of Internal Medicine, Division of Medical Oncology, The Ohio State University Comprehensive Cancer Center, Columbus, OH, United States. FAU - Dandawate, Prasad AU - Dandawate P AD - Department of Cancer Biology, University of Kansas Medical Center, Kansas City, KS, United States. FAU - Saha, Subhrajit AU - Saha S AD - Department of Cancer Biology, University of Kansas Medical Center, Kansas City, KS, United States. FAU - Schmitt, Timothy AU - Schmitt T AD - Department of Surgery, University of Kansas Medical Center, Kansas City, KS, United States. FAU - Kumer, Sean AU - Kumer S AD - Department of Surgery, University of Kansas Medical Center, Kansas City, KS, United States. FAU - King, Clay AU - King C AD - Department of Surgery, University of Kansas Medical Center, Kansas City, KS, United States. FAU - Olyaee, Mojtaba AU - Olyaee M AD - Division of Gastroenterology, Department of Internal Medicine, University of Kansas Medical Center, Kansas City, KS, United States. FAU - Rastogi, Amit AU - Rastogi A AD - Division of Gastroenterology and Hepatology, University of Kansas Medical Center, Kansas City, KS, United States. FAU - Hejazi, Reza AU - Hejazi R AD - Division of Gastroenterology and Hepatology, University of Kansas Medical Center, Kansas City, KS, United States. FAU - Sun, Weijing AU - Sun W AD - Department of Internal Medicine, Division of Medical Oncology, University of Kansas Medical Center, Westwood, KS, United States. FAU - Kasi, Anup AU - Kasi A AD - Department of Internal Medicine, Division of Medical Oncology, University of Kansas Medical Center, Westwood, KS, United States. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Cell Dev Biol JT - Frontiers in cell and developmental biology JID - 101630250 PMC - PMC13710092 OTO - NOTNLM OT - KRAS wild-type OT - PacC OT - genomic profiling OT - molecular profiling OT - pancreatic acinar cell carcinoma OT - precision oncology OT - rare pancreatic cancer OT - targeted therapy COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/13 CRDT- 2026/10/28 04:59 PHST- 2026/06/19 00:00 [received] PHST- 2026/08/12 00:00 [revised] PHST- 2026/08/19 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:59 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 1913398 [pii] AID - 10.3389/fcell.2026.1913398 [doi] PST - epublish SO - Front Cell Dev Biol. 2026 Oct 13;14:1913398. doi: 10.3389/fcell.2026.1913398. eCollection 2026. PMID- 42947886 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2297-1769 (Print) IS - 2297-1769 (Electronic) IS - 2297-1769 (Linking) VI - 13 DP - 2026 TI - Nutritional, metabolic, and intestinal responses to dried Opuntia ficus-indica cladode powder in growing rabbits. PG - 1991356 LID - 10.3389/fvets.2026.1991356 [doi] LID - 1991356 AB - Opuntia ficus-indica cladodes contain fiber and bioactive compounds, but their functional value at moderate inclusion levels in rabbit diets remains unclear. This study evaluated the effects of dried O. ficus-indica cladode powder on nutrient utilization, redox status, lipid metabolism, caecal characteristics, and duodenal morphology in growing rabbits. Seventy male Italian White rabbits were randomly assigned at 42 days of age to a control diet (CON) or a diet containing 50 g/kg, as-fed, of dried cladode powder (OP5; n = 35 per treatment) and fed until 84 days of age. Dietary OP5 did not affect growth performance, feed conversion ratio, slaughter traits, or meat proximate composition (p > 0.05). However, crude fiber and neutral detergent fiber digestibility increased by approximately 23 and 18%, respectively (p < 0.001). Serum total antioxidant capacity increased by 54% (p = 0.001), whereas malondialdehyde, total cholesterol, and triglycerides decreased (p /= 30 min/day) were associated with small improvements in friendship quantity and quality, whereas stricter limits (use < 30 min/day) yielded no benefit. At baseline, resilience was positively linked to friendship quantity and quality but did not predict or moderate T1-T2 change. Among continued users, T2 friendship quality (controlling T1) was positively associated with communication motives and negatively with selective restriction, while quantity showed no motive links. Overall, resilience appears to be a general resource for friendship quality and may buffer motive-relationship links. Understanding youths' SM motives may promote healthier use and guide ethical platforms and education design, especially for vulnerable youth. CI - Copyright (c) 2026 Schowalter, Reiter, Klumper and Agthe. FAU - Schowalter, Karina AU - Schowalter K AD - UMIT TIROL-Private University for Health Sciences and Health Technology, Institute for Psychology, Hall in Tirol, Austria. FAU - Reiter, Patrik AU - Reiter P AD - UMIT TIROL-Private University for Health Sciences and Health Technology, Institute for Psychology, Hall in Tirol, Austria. FAU - Klumper, Lisa AU - Klumper L AD - Department of Psychology, University of Wuppertal, Wuppertal, Nordrhein-Westfalen, Germany. FAU - Agthe, Maria AU - Agthe M AD - UMIT TIROL-Private University for Health Sciences and Health Technology, Institute for Psychology, Hall in Tirol, Austria. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Psychol JT - Frontiers in psychology JID - 101550902 PMC - PMC13710257 OTO - NOTNLM OT - communication OT - friendship quality OT - friendship quantity OT - psychological resilience OT - social media reduction OT - social media usage motives OT - youth COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/13 CRDT- 2026/10/28 04:50 PHST- 2026/08/08 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:50 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fpsyg.2026.1962459 [doi] PST - epublish SO - Front Psychol. 2026 Oct 13;17:1962459. doi: 10.3389/fpsyg.2026.1962459. eCollection 2026. PMID- 42947785 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Exercise capacity and handgrip strength following dumbbell-assisted diaphragmatic breathing training in patients with bronchiolitis obliterans syndrome after allogeneic hematopoietic stem cell transplantation: a prospective single-arm study. PG - 1948656 LID - 10.3389/fmed.2026.1948656 [doi] LID - 1948656 AB - BACKGROUND: Bronchiolitis obliterans syndrome (BOS) is a severe complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and is associated with impaired exercise capacity. Feasible rehabilitation strategies for this population remain limited. OBJECTIVES: To evaluate changes in functional exercise capacity and handgrip strength following a 12-week dumbbell-assisted diaphragmatic breathing training program in patients with BOS after allo-HSCT and to explore associations of these changes with selected baseline characteristics. METHODS: This prospective, single-center, single-arm pre-post interventional study included 31 patients with BOS after allo-HSCT. Participants completed a 12-week dumbbell-assisted diaphragmatic breathing training program. Six-minute walk distance (6MWD) and bilateral handgrip strength were assessed at baseline and week 12. Within-participant changes were evaluated using paired tests according to the distribution of change scores. Associations with selected baseline characteristics were examined in exploratory analyses. RESULTS: Following the 12-week intervention, mean 6MWD increased from 406.3 m at baseline to 425.9 m at week 12, corresponding to a mean within-participant increase of 19.6 m (95% CI: 9.3-29.9 m; p < 0.001). Mean bilateral handgrip strength increased from 23.2 kg to 25.0 kg, corresponding to a mean increase of 1.8 kg (95% CI: 1.3-2.3 kg; p < 0.001). The change in 6MWD did not differ significantly between women and men (p = 0.282), whereas the increase in handgrip strength was greater in women than in men (p = 0.021). Baseline heart-rate response during the 6MWT and supplemental oxygen requirement were not clearly associated with the magnitude of functional change. No serious training-related adverse events were observed. CONCLUSIONS: Twelve weeks of dumbbell-assisted diaphragmatic breathing training was associated with improvements in 6MWD and handgrip strength in patients with BOS after allo-HSCT. However, the single-arm design precludes causal attribution of these improvements to the intervention, and the exploratory subgroup findings should be interpreted cautiously. Larger controlled studies are needed to establish efficacy and characterize potential differences in response. CI - (c) 2026 Yuan, Ge, Zhu, Chen, Jiang and Zhang. FAU - Yuan, Xinyu AU - Yuan X AD - Department of Nursing, Jinzhou Medical University, Jinzhou, Liaoning, China. AD - Department of Respiratory and Critical Care Medicine, The Fourth Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. FAU - Ge, Wei AU - Ge W AD - Department of Respiratory and Critical Care Medicine, The Fourth Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. FAU - Zhu, Xiaojie AU - Zhu X AD - Department of Respiratory and Critical Care Medicine, The Fourth Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. FAU - Chen, Yunxia AU - Chen Y AD - Department of Respiratory and Critical Care Medicine, The Fourth Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. FAU - Jiang, Junhong AU - Jiang J AD - Department of Respiratory and Critical Care Medicine, The Fourth Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. FAU - Zhang, Huijun AU - Zhang H AD - Department of Nursing, Jinzhou Medical University, Jinzhou, Liaoning, China. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13710240 OTO - NOTNLM OT - 6MWT OT - BOS OT - allo-HSCT OT - dumbbell-assisted diaphragmatic breathing training OT - pulmonary rehabilitation COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/13 CRDT- 2026/10/28 04:50 PHST- 2026/07/25 00:00 [received] PHST- 2026/09/01 00:00 [revised] PHST- 2026/09/04 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:50 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fmed.2026.1948656 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 13;13:1948656. doi: 10.3389/fmed.2026.1948656. eCollection 2026. PMID- 42947784 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2234-943X (Print) IS - 2234-943X (Electronic) IS - 2234-943X (Linking) VI - 16 DP - 2026 TI - Case Report: Clinicopathologic and molecular characteristics of prostate adenocarcinoma brain metastases despite disproportionately low PSA levels: a four-patient case series and narrative review. PG - 1905004 LID - 10.3389/fonc.2026.1905004 [doi] LID - 1905004 AB - Brain metastases (BM) from prostate cancer (PCa) are rare, typically occurring in a small subset of patients with advanced disease or tumors with neuroendocrine differentiation. Diagnosis can be particularly challenging when prostate-specific antigen (PSA) levels remain low or undetectable. We describe four patients with prostate adenocarcinoma who developed brain metastases despite low or modestly elevated PSA levels relative to disease burden(<0.01 to 6.5 ng/ml). All patients underwent surgical resection followed by focal stereotactic radiosurgery and androgen deprivation therapy, with androgen receptor pathway inhibitors used in selected cases. Molecular profiling was available for three patients and demonstrated heterogeneous genomic findings, including PTEN alteration (Case 1), CDK4 amplification (Case 2), and an ATM mutation (Case 3), while testing was unavailable in Case 4. No pathogenic TP53 or RB1 alterations were identified in the profiled tumors. Two patients achieved durable biochemical and radiographic complete remission, one remained in remission before dying of an unrelated cause, and one developed biochemical recurrence without radiographic progression. These findings highlight the biological heterogeneity of central nervous system involvement in prostate cancer and emphasize that brain metastases should be considered even in patients with low PSA levels. Further studies are needed to better define the molecular determinants and biomarkers associated with this uncommon presentation. CI - Copyright (c) 2026 Bardhan, Myint, Kapoor and Selene. FAU - Bardhan, Roshmita AU - Bardhan R AD - Department of Internal Medicine, The Jewish Hospital-Mercy Health, Cincinnati, OH, United States. FAU - Myint, Zin W AU - Myint ZW AD - Internal Medicine/Medical Oncology Division, University of Kentucky, Kentucky, KY, United States. FAU - Kapoor, Harit AU - Kapoor H AD - Radiology, University of Kentucky, Kentucky, KY, United States. FAU - Selene, Insija AU - Selene I AD - Internal Medicine/medical Oncology Division, University of Kentucky, Kentucky, KY, United States. LA - eng PT - Case Reports PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Oncol JT - Frontiers in oncology JID - 101568867 PMC - PMC13710054 OTO - NOTNLM OT - androgen deprivation OT - brain metastases OT - molecular profiling OT - prostate adenocarcinoma OT - prostate specific antigen COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/13 CRDT- 2026/10/28 04:50 PHST- 2026/06/10 00:00 [received] PHST- 2026/07/28 00:00 [revised] PHST- 2026/08/03 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:50 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fonc.2026.1905004 [doi] PST - epublish SO - Front Oncol. 2026 Oct 13;16:1905004. doi: 10.3389/fonc.2026.1905004. eCollection 2026. PMID- 42947782 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-2392 (Print) IS - 1664-2392 (Electronic) IS - 1664-2392 (Linking) VI - 17 DP - 2026 TI - Modulation of immune and inflammatory responses involving selected galectins and selected immune-inflammatory mediators in women with deep endometriosis coexisting with adenomyosis - preliminary study. PG - 1920315 LID - 10.3389/fendo.2026.1920315 [doi] LID - 1920315 AB - INTRODUCTION: Endometriosis is characterized by the presence of functionally active endometrial tissue outside the uterine cavity, primarily within the peritoneal cavity. Its etiology and pathogenesis remain incompletely understood; however, endometriosis is considered a chronic inflammatory disease associated with immune system dysfunction. Galectins and other mediators of immune and inflammatory responses may play an important role in the pathogenesis of the disease. This study aimed to investigate the potential involvement of selected immune and inflammatory mediators in endometriosis by assessing the concentrations of galectin-1 (Gal-1), galectin-3 (Gal-3), galectin-8 (Gal-8), galectin-9 (Gal-9), asprosin, FSTL1, meteorin, neuregulin-4, isthmin 1, SFRP2, and TNF-alpha in the serum and peritoneal fluid of women with deep infiltrating endometriosis, deep infiltrating endometriosis coexisting with adenomyosis, and adenomyosis. METHODS: The study included 33 women in whom endometriosis was diagnosed during laparoscopy and subsequently confirmed by histopathological examination. The reference group consisted of 14 women without endometriosis. Blood serum and peritoneal fluid samples were collected, and the concentrations of the investigated parameters were determined using enzyme-linked immunosorbent assays (ELISAs). RESULTS: In patients with deep infiltrating endometriosis coexisting with adenomyosis, alterations in the detectability of Gal-1, Gal-3, and Gal-9 were observed, suggesting a potential involvement of these galectins in the regulation of immune and inflammatory responses. Differences in the concentrations of asprosin, FSTL1, and TNF-alpha were also observed in this group, suggesting a possible association of these parameters with the inflammatory environment accompanying the disease. CONCLUSIONS: The observed alterations in Gal-1, Gal-3, and Gal-9 levels in patients with deep infiltrating endometriosis coexisting with adenomyosis may indicate their involvement in the regulation of immune and inflammatory responses. Differences in the concentrations of asprosin, FSTL1, and TNF-alpha suggest their potential association with the inflammatory environment of the disease. Further studies in larger cohorts are needed to determine the biological and clinical significance of these parameters and their potential interactions. CI - Copyright (c) 2026 Wendlocha, Smycz-Kubanska, Koscielniak-Ziemniak, Wojtowicz and Mielczarek-Palacz. FAU - Wendlocha, Dominika AU - Wendlocha D AD - Department of Immunology and Serology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Katowice, Poland. FAU - Smycz-Kubanska, Marta AU - Smycz-Kubanska M AD - Department of Immunology and Serology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Katowice, Poland. FAU - Koscielniak-Ziemniak, Magdalena AU - Koscielniak-Ziemniak M AD - Medical Diagnostic Laboratory City Hospital in Zabrze, Zabrze, Poland. FAU - Wojtowicz, Mariusz AU - Wojtowicz M AD - Clinical Department of Gynecology and Obstetrics, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, Katowice, Poland. FAU - Mielczarek-Palacz, Aleksandra AU - Mielczarek-Palacz A AD - Department of Immunology and Serology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Katowice, Poland. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Endocrinol (Lausanne) JT - Frontiers in endocrinology JID - 101555782 RN - 0 (Galectins) RN - 0 (Inflammation Mediators) RN - 0 (Biomarkers) SB - IM MH - Humans MH - Female MH - *Endometriosis/immunology/complications/metabolism/pathology MH - *Adenomyosis/immunology/complications/metabolism/pathology MH - Adult MH - *Galectins/metabolism/blood MH - Ascitic Fluid/metabolism MH - *Inflammation/metabolism/immunology/pathology MH - *Inflammation Mediators/metabolism/blood MH - Biomarkers/metabolism PMC - PMC13710056 OTO - NOTNLM OT - adenomyosis OT - deep infiltrating endometriosis OT - galectins OT - immune response OT - inflammation COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 12:34 PMCR- 2026/10/13 CRDT- 2026/10/28 04:50 PHST- 2026/06/26 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/28 12:34 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:50 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fendo.2026.1920315 [doi] PST - epublish SO - Front Endocrinol (Lausanne). 2026 Oct 13;17:1920315. doi: 10.3389/fendo.2026.1920315. eCollection 2026. PMID- 42947781 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-2360 (Print) IS - 2296-2360 (Electronic) IS - 2296-2360 (Linking) VI - 14 DP - 2026 TI - Reported infant safety information and perinatal service engagement among birth partners and co-parents. An exploratory cross-sectional survey at a large UK teaching hospital. PG - 1884421 LID - 10.3389/fped.2026.1884421 [doi] LID - 1884421 AB - BACKGROUND: Infant mortality in the United Kingdom is higher in areas of socioeconomic deprivation and within several minority ethnic groups. Preventive strategies rely in part on safety information reaching families during the perinatal period. The extent to which birth partners and co-parents report exposure to that information is not well characterised. OBJECTIVE: To describe self-reported exposure to selected infant safety topics and reported engagement with perinatal services among birth partners and co-parents attending one large UK teaching hospital, and to explore variation across respondent profiles defined by ethnicity and parity. METHODS: An exploratory cross-sectional survey of 73 birth partners and co-parents was conducted at University Hospitals Leicester NHS Trust between October 2022 and July 2023. Eight self-reported knowledge or awareness items and six self-reported engagement items were collected. Item-level proportions were summarised with Wilson 95 percent confidence intervals. Group comparisons used Fisher's exact test with Haldane-corrected prevalence ratios and Benjamini-Hochberg adjustment applied separately within each demographic comparison. Composite Knowledge Proportion Scores and Engagement Proportion Scores were computed for respondents with at least half of the relevant items answered. Internal consistency was estimated with the Kuder-Richardson 20 coefficient, association with Spearman's rank correlation, and group differences with the Mann-Whitney U test. Reporting follows the STROBE statement for cross-sectional studies. RESULTS: Reported exposure was highest for smoking risk (69.9 percent, 95 percent CI 58.6-79.2) and lowest for basic bystander life support (17.8 percent, 95 percent CI 10.7-28.1). Engagement was highest for NHS website use and post-birth breastfeeding support (both 61.6 percent, 95 percent CI 50.2-71.9) and lowest for antenatal classes (24.7 percent, 95 percent CI 16.2-35.6). Composite scales showed high internal consistency (KR-20 of 0.94 for knowledge, 0.93 for engagement). The two composite scores were strongly correlated (Spearman's rho = 0.965, p < 0.001). Ethnicity and parity were completely confounded in the usable sample, with all first-time parents identifying as White British and all respondents in the combined Other category identifying as experienced parents. Observed differences between these respondent profiles cannot be attributed independently to ethnicity or parity. CONCLUSIONS: In this exploratory single-site sample, self-reported exposure to practical infant emergency-care topics was low. Substantial differences were observed between two contrasting respondent profiles, although the confounded design and small convenience sample do not support causal claims about ethnic inequities or infant mortality risk reduction. The findings are hypothesis-generating and support the case for larger, more diverse studies designed to separate the contributions of ethnicity, parity, and other social determinants. CI - (c) 2026 Olakotan, Syed, Abudraz, Benouali, Ali, Chua, Takar, Miller, Dodwell, Mogaji, Rashwan, Ahmed, Sivakumaran, O'Kerr, Atiku, Khare and Pillay. FAU - Olakotan, Olufisayo AU - Olakotan O AD - Department of Research and Development, The Royal Wolverhampton NHS Trust, Wolverhampton, United Kingdom. FAU - Syed, Modther AU - Syed M AD - Department of Neonatology, University Hospitals Leicester NHS Trust, Leicester, United Kingdom. FAU - Abudraz, Ahmed AU - Abudraz A AD - Department of Neonatology, University Hospitals Leicester NHS Trust, Leicester, United Kingdom. FAU - Benouali, Camilla AU - Benouali C AD - Department of Neonatology, University Hospitals Leicester NHS Trust, Leicester, United Kingdom. FAU - Ali, Muhammad AU - Ali M AD - Department of Neonatology, University Hospitals Leicester NHS Trust, Leicester, United Kingdom. FAU - Chua, Nicole AU - Chua N AD - Department of Neonatology, University Hospitals Leicester NHS Trust, Leicester, United Kingdom. FAU - Takar, Su'ad AU - Takar S AD - Department of Neonatology, University Hospitals Leicester NHS Trust, Leicester, United Kingdom. FAU - Miller, Theubo AU - Miller T AD - Department of Neonatology, University Hospitals Leicester NHS Trust, Leicester, United Kingdom. FAU - Dodwell, William AU - Dodwell W AD - Department of Neonatology, University Hospitals Leicester NHS Trust, Leicester, United Kingdom. FAU - Mogaji, Fatima Tiamiyu AU - Mogaji FT AD - Department of Neonatology, University Hospitals Leicester NHS Trust, Leicester, United Kingdom. FAU - Rashwan, Sanaa AU - Rashwan S AD - Department of Neonatology, University Hospitals Leicester NHS Trust, Leicester, United Kingdom. FAU - Ahmed, Elmansi AU - Ahmed E AD - Department of Neonatology, University Hospitals Leicester NHS Trust, Leicester, United Kingdom. FAU - Sivakumaran, Priya AU - Sivakumaran P AD - Department of Obstetrics, University Hospitals Leicester NHS Trust, Leicester, United Kingdom. FAU - O'Kerr, Megan AU - O'Kerr M AD - Department of Obstetrics, University Hospitals Leicester NHS Trust, Leicester, United Kingdom. FAU - Atiku, Samuel AU - Atiku S AD - Research Service, Aston University, Birmingham, United Kingdom. FAU - Khare, Manjiri AU - Khare M AD - Department of Obstetrics, University Hospitals Leicester NHS Trust, Leicester, United Kingdom. AD - Department of Population Health Sciences, University of Leicester, Leicester, United Kingdom. FAU - Pillay, Thillagavathie AU - Pillay T AD - Department of Research and Development, The Royal Wolverhampton NHS Trust, Wolverhampton, United Kingdom. AD - Department of Neonatology, University Hospitals Leicester NHS Trust, Leicester, United Kingdom. AD - Department of Population Health Sciences, University of Leicester, Leicester, United Kingdom. AD - Faculty of Science and Education, University of Wolverhampton, Wolverhampton, United Kingdom. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Pediatr JT - Frontiers in pediatrics JID - 101615492 PMC - PMC13710227 OTO - NOTNLM OT - STROBE OT - birth partner OT - co-parent OT - health inequalities OT - perinatal education OT - self-reported knowledge COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/13 CRDT- 2026/10/28 04:50 PHST- 2026/05/18 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:50 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fped.2026.1884421 [doi] PST - epublish SO - Front Pediatr. 2026 Oct 13;14:1884421. doi: 10.3389/fped.2026.1884421. eCollection 2026. PMID- 42947769 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-2565 (Electronic) IS - 2296-2565 (Linking) VI - 14 DP - 2026 TI - Effects of exercise-based interventions on balance and functional mobility in adults with chronic stroke: a systematic review and meta-analysis. PG - 1930987 LID - 10.3389/fpubh.2026.1930987 [doi] LID - 1930987 AB - OBJECTIVE: To systematically evaluate, using the GRADE framework, the effects of exercise-based interventions on Berg Balance Scale, Timed Up and Go, and Functional Reach Test outcomes in adults with chronic stroke. METHODS: We systematically searched PubMed, Embase, Web of Science, Scopus, and the Physiotherapy Evidence Database from inception to 29 June 2025 for randomized controlled trials investigating structured exercise-based interventions in adults with chronic stroke. Eligible studies enrolled adults at least 6 months after stroke onset and reported at least one validated balance or functional mobility outcome. Methodological quality was assessed using the Physiotherapy Evidence Database scale, and the certainty of evidence was rated using the GRADE approach. Meta-analyses were performed using random-effects models and expressed as mean differences (MDs) or standardized mean differences (SMDs) with 95% confidence intervals (CIs); exploratory univariable random-effects meta-regression was used to examine intervention duration and training frequency. RESULTS: Sixty-nine randomized controlled trials involving 2,314 adults with chronic stroke were included. Exercise-based interventions improved Berg Balance Scale scores [MD = 2.54, 95% CI (1.83-3.26)], reduced Timed Up and Go test completion time [MD = -2.68 s, 95% CI (-3.72 to -1.65)], and improved Functional Reach Test performance [SMD = 0.65, 95% CI (0.25-1.05)]. The certainty of evidence was moderate for BBS, high for TUG, and moderate for FRT, with the degree of heterogeneity differing across outcomes. CONCLUSION: Exercise-based interventions were associated with improvements in BBS scores, TUG performance, and FRT outcomes in adults with chronic stroke, although the certainty and consistency of evidence varied across these measures. These findings support the potential role of exercise-based interventions in improving these selected outcomes. Larger pragmatic trials with standardized intervention reporting and longer follow-up are needed to clarify optimal training components, dose, delivery, and durability of effects. SYSTEMATIC REVIEW REGISTRATION: https://www.crd.york.ac.uk/PROSPERO/view/CRD42020156992, identifier CRD42020156992. CI - Copyright (c) 2026 Zhan, Bai, Pan, Li and Wang. FAU - Zhan, Yating AU - Zhan Y AD - School of Nursing, University of South China, Hengyang, Hunan, China. FAU - Bai, Hao AU - Bai H AD - School of Nursing, University of South China, Hengyang, Hunan, China. FAU - Pan, Tongtong AU - Pan T AD - School of Nursing, University of South China, Hengyang, Hunan, China. FAU - Li, Yue AU - Li Y AD - School of Nursing, University of South China, Hengyang, Hunan, China. FAU - Wang, Rong AU - Wang R AD - School of Nursing, University of South China, Hengyang, Hunan, China. LA - eng PT - Journal Article PT - Meta-Analysis PT - Systematic Review DEP - 20261013 PL - Switzerland TA - Front Public Health JT - Frontiers in public health JID - 101616579 SB - IM MH - Humans MH - *Postural Balance/physiology MH - *Stroke Rehabilitation/methods MH - *Exercise Therapy/methods MH - Chronic Disease MH - *Stroke/therapy MH - Randomized Controlled Trials as Topic PMC - PMC13710241 OTO - NOTNLM OT - chronic stroke OT - exercise therapy OT - functional mobility OT - meta-analysis OT - physical therapy OT - postural balance OT - public health OT - rehabilitation COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 12:34 PMCR- 2026/10/13 CRDT- 2026/10/28 04:49 PHST- 2026/07/07 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/28 12:34 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:49 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fpubh.2026.1930987 [doi] PST - epublish SO - Front Public Health. 2026 Oct 13;14:1930987. doi: 10.3389/fpubh.2026.1930987. eCollection 2026. PMID- 42947758 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261028 IS - 2692-8205 (Electronic) IS - 2692-8205 (Linking) DP - 2026 Oct 18 TI - Gut microbially produced phenolic acids have differential and sex-dependent effects on host metabolic health. LID - 2026.10.12.757096 [pii] LID - 10.64898/2026.10.12.757096 [doi] AB - Gut microbiota-derived metabolites are increasingly recognized as important regulators of host metabolism, but the specific functions of individual metabolites remain poorly understood. We therefore investigated the metabolic effects of two structurally related, phenylalanine-derived microbial metabolites, phenylacetic acid (PAA) and phenylpropionic acid (PPA), in a mouse model of diet-induced obesity. Although clinical studies have linked PAA and its downstream product phenylacetylglutamine to metabolic disease, PAA did not alter body mass or adiposity in this model. Instead, it improved glucose tolerance selectively in male mice and markedly remodeled lipid metabolism in both sexes, reducing circulating triglycerides and acylcarnitines and activating hepatic PPAR-alpha-associated transcriptional programs associated with fatty acid omega-hydroxylation and peroxisomal lipid metabolism. Despite this extensive metabolic remodeling, PAA did not worsen hepatic steatosis, fibrosis, or markers of liver injury. By contrast, PPA had minimal effects on hepatic metabolic pathways but produced pronounced sex-dependent physiological responses, increasing adiposity in female mice and improving glucose tolerance in male mice. These findings show that structurally related microbial metabolites can have distinct, sex-dependent metabolic effects and highlight the need to define the actions of individual microbiome-derived metabolites when developing targeted therapies for metabolic disease. FAU - Kim, Jiyeon AU - Kim J FAU - Carr, Kelley M AU - Carr KM FAU - Alqudah, Sara AU - Alqudah S FAU - Bacik, Kathryn A AU - Bacik KA FAU - Park, Hannah AU - Park H FAU - Fulmer, Clifton G AU - Fulmer CG FAU - Awoniyi, Muyiwa AU - Awoniyi M FAU - Claesen, Jan AU - Claesen J FAU - Nemet, Ina AU - Nemet I AUID- ORCID: 0000-0002-0657-7121 LA - eng PT - Journal Article PT - Preprint DEP - 20261018 PL - United States TA - bioRxiv JT - bioRxiv : the preprint server for biology JID - 101680187 PMC - PMC13701097 EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/26 CRDT- 2026/10/28 04:49 PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:49 [entrez] PHST- 2026/10/26 00:00 [pmc-release] AID - 2026.10.12.757096 [pii] AID - 10.64898/2026.10.12.757096 [doi] PST - epublish SO - bioRxiv [Preprint]. 2026 Oct 18:2026.10.12.757096. doi: 10.64898/2026.10.12.757096. PMID- 42947743 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2331-8325 (Electronic) IS - 2331-8325 (Linking) VI - 16 IP - 20 DP - 2026 Oct 20 TI - Disease Modeling in iPSC-Derived Human Bone Marrow Organoids. PG - e5837 LID - 10.21769/BioProtoc.5837 [doi] LID - e5837 AB - Human bone marrow organoids provide a tractable three-dimensional platform for modeling hematopoiesis and hematologic disease in a human niche-like context. Here, we describe a stepwise protocol for utilizing human induced pluripotent stem cell (iPSC)-derived bone marrow organoids that support autonomous hematopoiesis for hematopoietic disease modeling, mouse xenograft hematopoiesis, and drug sensitivity testing. The workflow combines embryoid body formation, early mesoderm/angiogenic induction under hypoxia, hemogenic endothelial commitment, maturation within a collagen-containing hydrogel, and subsequent suspension culture as individual organoids. The resulting organoids contain endothelial, stromal, and hematopoietic components and reproduce key structural and cellular features of human marrow. We further describe procedures for engraftment of normal donor- or patient-derived CD34+ cells and implantation of mature organoids under the renal capsule of immunodeficient mice to assess in vivo hematopoietic maintenance. In prior applications of this platform, donor-derived CD34+ cells were shown to engraft within the organoid niche and undergo multilineage differentiation, enabling detection of selective erythroid defects caused by DDX41 deficiency and assessment of therapeutic suppression of JAK2V617F-mutant patient-derived hematopoietic cells in a human marrow-like microenvironment. This protocol, therefore, enables disease modeling, in vivo xenograft assessment, and ex vivo functional analysis of patient-derived hematopoietic cells using relatively small input samples. Key features * Stepwise differentiation of human iPSCs into bone marrow organoids containing endothelial, stromal, and hematopoietic compartments. * Efficient engraftment of donor-derived CD34+ HSPCs into mature organoids using a Matrigel-assisted seeding method. * Applicable to imaging, flow cytometry, cytogenetics, and downstream molecular analyses of organoid and donor-derived hematopoietic cells. * Includes renal capsule implantation for in vivo xenograft assessment of organoid-supported hematopoiesis. * Enables disease modeling and ex vivo functional studies of patient-derived hematopoietic cells, including analysis of erythroid and myeloid phenotypes and therapeutic responses. CI - (c)Copyright : (c) 2026 The Authors; This is an open access article under the CC BY-NC license. FAU - Li, Ermin AU - Li E AD - Division of Hematology and Oncology, Department of Medicine, Stony Brook University, Stony Brook, NY, USA. AD - Stony Brook Cancer Center, Stony Brook, NY, USA. AD - Department of Microbiology & Immunology, Stony Brook University, Stony Brook, NY, USA. FAU - Ji, Amy AU - Ji A AD - Columbia College, Columbia University, New York, NY, USA. FAU - Zheng, Ashley AU - Zheng A AD - Division of Hematology and Oncology, Department of Medicine, Stony Brook University, Stony Brook, NY, USA. FAU - Ren, Kehan AU - Ren K AD - Division of Hematology and Oncology, Department of Medicine, Stony Brook University, Stony Brook, NY, USA. AD - Stony Brook Cancer Center, Stony Brook, NY, USA. AD - Department of Microbiology & Immunology, Stony Brook University, Stony Brook, NY, USA. LA - eng PT - Journal Article DEP - 20261020 PL - United States TA - Bio Protoc JT - Bio-protocol JID - 101635102 PMC - PMC13710131 OTO - NOTNLM OT - Acute myeloid leukemia OT - Bone marrow organoid OT - CD34 OT - DDX41 OT - Drug sensitivity testing. OT - Engraftment OT - Erythropoiesis OT - JAK2 OT - Myelodysplastic syndrome OT - Myeloproliferative neoplasms OT - iPSC-derived hematopoiesis COIS- Competing interestsK.R. and E.L. are co-founders of MarrowGen and co-inventors on patent application US20260071185A1 related to bone marrow organoid technology. The remaining authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/20 CRDT- 2026/10/28 04:48 PHST- 2026/04/20 00:00 [received] PHST- 2026/09/02 00:00 [revised] PHST- 2026/09/04 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:48 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - e5837 [pii] AID - 5837 [pii] AID - 10.21769/BioProtoc.5837 [doi] PST - epublish SO - Bio Protoc. 2026 Oct 20;16(20):e5837. doi: 10.21769/BioProtoc.5837. eCollection 2026 Oct 20. PMID- 42947728 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 0277-786X (Print) IS - 1996-756X (Electronic) IS - 0277-786X (Linking) VI - 13927 DP - 2026 TI - Design and evaluation of a deformable multi-organ abdominal phantom for multimodality imaging applications. LID - 139272C [pii] LID - 10.1117/12.3086211 [doi] AB - A deformable multi-organ abdominal phantom can have many applications in medical imaging and image-guided interventions. It can provide ground truth data for registration evaluation and procedure training. In this study, we designed and fabricated a deformable abdominal phantom for multimodal imaging applications. Computed tomography (CT) images of a human patient were used to segment the abdominal surface, kidneys, spleen, and liver. Scaled down 3D-printed molds were created based on the segmented 3D models of these structures. To allow for deformability, an agar-gelatin gel mixture was used to cast the phantom using the 3D-printed molds with potassium iodide added to the target formulation as a CT contrast agent. Psyllium husk powder was utilized as an ultrasound (US) scattering agent. Simulated tumors or targets were incorporated into the kidney phantom. All components were assembled into an abdominal mold to replicate patient anatomical structures. The phantom was evaluated using CT and ultrasound imaging, with preliminary results demonstrating its ability to provide ground truth for tumor localization. This study presents a reproducible framework for creating a deformable abdominal phantom that can be applied to the development and validation of image-guided interventional procedures. FAU - Yuan, Mandy AU - Yuan M AD - Center for Imaging and Surgical Innovation, University of Texas at Dallas, Richardson, TX. AD - Department of Bioengineering, University of Texas at Dallas, Richardson, TX. FAU - Nawawithan, Nati AU - Nawawithan N AD - Center for Imaging and Surgical Innovation, University of Texas at Dallas, Richardson, TX. AD - Department of Bioengineering, University of Texas at Dallas, Richardson, TX. FAU - Yu, James AU - Yu J AD - Center for Imaging and Surgical Innovation, University of Texas at Dallas, Richardson, TX. AD - Department of Radiology, University of Texas Southwestern Medical Center, Dallas, TX. FAU - Patwardhan, Chinmay AU - Patwardhan C AD - Center for Imaging and Surgical Innovation, University of Texas at Dallas, Richardson, TX. AD - Department of Bioengineering, University of Texas at Dallas, Richardson, TX. FAU - Swain, Suhani AU - Swain S AD - Center for Imaging and Surgical Innovation, University of Texas at Dallas, Richardson, TX. AD - Department of Bioengineering, University of Texas at Dallas, Richardson, TX. FAU - Chaverra, Izabella AU - Chaverra I AD - Center for Imaging and Surgical Innovation, University of Texas at Dallas, Richardson, TX. AD - Department of Bioengineering, University of Texas at Dallas, Richardson, TX. FAU - Fei, Baowei AU - Fei B AD - Center for Imaging and Surgical Innovation, University of Texas at Dallas, Richardson, TX. AD - Department of Bioengineering, University of Texas at Dallas, Richardson, TX. AD - Department of Radiology, University of Texas Southwestern Medical Center, Dallas, TX. LA - eng GR - R01 CA288379/CA/NCI NIH HHS/United States PT - Journal Article DEP - 20260401 PL - United States TA - Proc SPIE Int Soc Opt Eng JT - Proceedings of SPIE--the International Society for Optical Engineering JID - 101524122 PMC - PMC13709972 MID - NIHMS2215309 OTO - NOTNLM OT - CT OT - Deformable phantom OT - minimally invasive interventions OT - multi-modality imaging OT - ultrasound imaging EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/28 CRDT- 2026/10/28 04:46 PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:46 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 139272C [pii] AID - 10.1117/12.3086211 [doi] PST - ppublish SO - Proc SPIE Int Soc Opt Eng. 2026;13927:139272C. doi: 10.1117/12.3086211. Epub 2026 Apr 1. PMID- 42947723 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1881-641X (Print) IS - 1881-6428 (Electronic) IS - 1881-641X (Linking) VI - 19 IP - 1 DP - 2026 TI - Mechanical and Hemodynamic Effects of Balloon-Expandable and Self-Expanding Stents in a Saccular Abdominal Aortic Aneurysm Model: Fluid-Structure Interaction Analyses. LID - 26-00052 [pii] LID - 10.3400/avd.oa.26-00052 [doi] AB - OBJECTIVES: The aim of this study was to compare the effects of balloon-expandable and self-expanding stents on vessel wall mechanics and hemodynamics in a patient-specific saccular abdominal aortic aneurysm model. METHODS: A patient-specific aneurysm model was constructed using computed tomography images, and balloon-expandable and self-expanding stent models were created. Aortic wall stress was evaluated by contact analysis. Fluid-structure interaction analyses were performed at 5 representative phases selected from the cardiac cycle. Mean intra-aneurysmal flow velocity, wall shear stress (WSS), pressure loss, and energy loss were compared. RESULTS: The balloon-expandable stent produced greater localized aortic wall stress than the self-expanding stent, whereas wall stress was more uniformly distributed with the self-expanding stent. Both stents reduced the mean intra-aneurysmal flow velocity, WSS, pressure loss, and energy loss compared with the no-stent model. However, greater reductions in WSS, pressure loss, and energy loss were noted with the balloon-expandable stent than with the self-expanding stent. CONCLUSIONS: Balloon-expandable and self-expanding stents exhibited different effects on vessel wall mechanics and hemodynamics. The findings indicate the importance of evaluating both hemodynamic and mechanical effects when assessing stent-vessel interactions. CI - (c) 2026 The Editorial Committee of Annals of Vascular Diseases. FAU - Murakami, Masanori AU - Murakami M AD - Department of Cardiovascular Surgery, National Hospital Organization Kanmon Medical Center, Shimonoseki, Yamaguchi, Japan. FAU - Jiang, Fei AU - Jiang F AD - Department of Mechanical Engineering, Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Ube, Yamaguchi, Japan. FAU - Yamashita, Tatsuo AU - Yamashita T AD - Department of Mechanical Engineering, Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Ube, Yamaguchi, Japan. FAU - Mochizuki, Shinsuke AU - Mochizuki S AD - Department of Mechanical Engineering, Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Ube, Yamaguchi, Japan. LA - eng PT - Journal Article DEP - 20261023 PL - Japan TA - Ann Vasc Dis JT - Annals of vascular diseases JID - 101471467 PMC - PMC13710210 OTO - NOTNLM OT - abdominal aortic aneurysm OT - balloon-expandable stent OT - fluid-structure interaction OT - hemodynamics OT - self-expanding stent COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/01/01 CRDT- 2026/10/28 04:45 PHST- 2026/04/05 00:00 [received] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:45 [entrez] PHST- 2026/01/01 00:00 [pmc-release] AID - 26-00052 [pii] AID - 10.3400/avd.oa.26-00052 [doi] PST - ppublish SO - Ann Vasc Dis. 2026;19(1):26-00052. doi: 10.3400/avd.oa.26-00052. Epub 2026 Oct 23. PMID- 42947720 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2378-8038 (Print) IS - 2378-8038 (Electronic) IS - 2378-8038 (Linking) VI - 11 IP - 5 DP - 2026 Oct TI - Trends in Tracheostomy Utilization, Tracheostomy Dependence, and Outcomes Before and After the COVID-19 Pandemic. PG - e70583 LID - 10.1002/lio2.70583 [doi] LID - e70583 AB - OBJECTIVES: The COVID-19 pandemic led to an unprecedented demand for invasive mechanical ventilation and may have affected tracheostomy utilization, dependence, and airway-related outcomes. However, population-level trends before and after the pandemic remain poorly characterized. We evaluated temporal trends in tracheostomy utilization, dependence, and airway-related complications among intubated and mechanically ventilated patients before versus after the COVID-19 pandemic. METHODS: We conducted a retrospective cohort study using de-identified data from the TriNetX U.S. Collaborative Network. Adults (>/= 18 years) who underwent endotracheal intubation and respiratory ventilation from 2016 to 2023 were included. Patients were stratified into pre-pandemic (2016-2019) and pandemic (2020-2023) cohorts. Propensity score matching was performed on 13 demographic and clinical covariates. Primary outcomes were tracheostomy and tracheostomy dependence, and secondary outcomes included airway-related complications. Risk ratios (RRs) with 95% confidence intervals (CIs) were calculated. RESULTS: Among 155,499 intubated and mechanically ventilated patients, the incidence proportions of tracheostomy, tracheostomy dependence, and dysphagia increased from 2016 to 2023 by approximately 2.1, 3.3, and 2.3 times, respectively. After matching (n = 64,732 per cohort), there was no difference in tracheostomy surgical rates between pre- and pandemic cohorts (RR: 0.97, 95% CI: 0.91-1.02). However, pandemic patients had higher risks of tracheostomy dependence (RR: 1.14, 95% CI: 1.08-1.21) and dysphagia (RR: 1.07, 95% CI: 1.04-1.10). CONCLUSION: Tracheostomy dependence and select airway complications increased in the pandemic period, suggesting lasting downstream effects of critical care practices after the pandemic. Proactive resource planning and long-term strategies for airway care in tracheotomized patients are needed. LEVEL OF EVIDENCE: 3. CI - (c) 2026 The Author(s). Laryngoscope Investigative Otolaryngology published by Wiley Periodicals LLC on behalf of The Triological Society. FAU - Williams-Medina, Eduardo AU - Williams-Medina E AUID- ORCID: 0000-0003-1704-6622 AD - Cleveland Clinic Lerner College of Medicine Case Western Reserve University Cleveland Ohio USA. FAU - Tierney, William S AU - Tierney WS AD - Department of Otolaryngology-Head and Neck Surgery Cleveland Clinic Head and Neck Institute Cleveland Ohio USA. LA - eng PT - Journal Article DEP - 20261026 PL - United States TA - Laryngoscope Investig Otolaryngol JT - Laryngoscope investigative otolaryngology JID - 101684963 PMC - PMC13709974 OTO - NOTNLM OT - Airway complications OT - COVID-19 pandemic OT - Tracheostomy OT - Trends COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/26 CRDT- 2026/10/28 04:45 PHST- 2026/05/16 00:00 [received] PHST- 2026/08/20 00:00 [revised] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:45 [entrez] PHST- 2026/10/26 00:00 [pmc-release] AID - LIO270583 [pii] AID - 10.1002/lio2.70583 [doi] PST - epublish SO - Laryngoscope Investig Otolaryngol. 2026 Oct 26;11(5):e70583. doi: 10.1002/lio2.70583. eCollection 2026 Oct. PMID- 42947719 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1662-5196 (Print) IS - 1662-5196 (Electronic) IS - 1662-5196 (Linking) VI - 20 DP - 2026 TI - Computational approaches to deep learning-driven EEG analysis for neurological disease diagnosis: a systematic review. PG - 1778980 LID - 10.3389/fninf.2026.1778980 [doi] LID - 1778980 AB - Electroencephalography (EEG) is a widely used non-invasive neurophysiological technique for analyzing brain dynamics and diagnosing neurological diseases. However, the non-stationary nature, low signal-to-noise ratio, and inter-subject variability of EEG signals cause significant challenges to traditional analytical approaches. In recent years, deep learning has emerged as a powerful computational tool for EEG analysis, which enables automated feature learning and improved diagnostic accuracy with minimal human-driven feature extraction. This review systematically explains computational approaches in deep learning-driven EEG analysis for the diagnosis of neurological diseases. It includes recent advances in deep learning architectures, such as convolutional neural networks (CNN), recurrent neural networks (RNN), hybrid configurations, Generative Adversarial Networks (GAN), Large Language Models (LLM), autoencoders, and transformer-based models, with a focus on their capacity to model spatial, temporal, and spatiotemporal dynamics in EEG signals. This is applicable in diagnosing major neurological disorders such as Epilepsy, Alzheimer's disease (AD), Encephalopathy, Parkinson's disease (PD), Mild Cognitive Impairment (MCI), sleep disorders, and developmental brain disorders. Furthermore, we discuss essential preprocessing strategies and denoising techniques, showing how removing biological artifacts prevents diagnostic models from confusing noise with actual signs of disease. Key challenges such as limited data availability, lack of model interpretability, inter-subject variability, and difficulty in clinical translation are identified and critically discussed. Finally, key emerging trends and future research directions are identified to support the development of clinical applications of EEG-based disease diagnostic systems. CI - Copyright (c) 2026 H F and Jacob. FAU - H F, Thazneem AU - H F T AD - Sree Chitra Thirunal College of Engineering, Pappanamcode, Thiruvananthapuram, Kerala, India. AD - Research Scholar, APJ Abdul Kalam Technological University, Kerala, India. FAU - Jacob, Jisu Elsa AU - Jacob JE AD - Sree Chitra Thirunal College of Engineering, Pappanamcode, Thiruvananthapuram, Kerala, India. LA - eng PT - Journal Article PT - Systematic Review DEP - 20261013 PL - Switzerland TA - Front Neuroinform JT - Frontiers in neuroinformatics JID - 101477957 PMC - PMC13710140 OTO - NOTNLM OT - EEG OT - EEG analysis methods OT - automated diagnosis OT - deep learning-artificial intelligence OT - machine learning-based classification COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/13 CRDT- 2026/10/28 04:45 PHST- 2025/12/31 00:00 [received] PHST- 2026/08/10 00:00 [revised] PHST- 2026/08/17 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:45 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fninf.2026.1778980 [doi] PST - epublish SO - Front Neuroinform. 2026 Oct 13;20:1778980. doi: 10.3389/fninf.2026.1778980. eCollection 2026. PMID- 42947712 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2296-858X (Print) IS - 2296-858X (Electronic) IS - 2296-858X (Linking) VI - 13 DP - 2026 TI - Case Report: Delayed hemobilia caused by hepatic artery pseudoaneurysm with arterio-biliary fistula after liver transplantation. PG - 1940000 LID - 10.3389/fmed.2026.1940000 [doi] LID - 1940000 AB - BACKGROUND: Hepatic artery pseudoaneurysm (HAP) is a rare but life-threatening complication following liver transplantation (LT). The formation of an arterio-biliary fistula leading to hemobilia is exceptionally uncommon, particularly when manifesting several months post-transplantation. Diagnosis and therapeutic management remain highly challenging because achieving definitive hemostasis often conflicts with the critical need to preserve graft arterial perfusion. CASE PRESENTATION: A 23-year-old male presented with recurrent hematemesis, hematochezia, and right upper quadrant pain 4 months after undergoing orthotopic LT for end-stage alcohol-related cirrhosis. While initial endoscopy pointed toward a duodenal bulb ulcer, a repeat endoscopic evaluation revealed fresh blood actively emerging from the major duodenal papilla, raising strong suspicion for hemobilia. Subsequent digital subtraction angiography (DSA) demonstrated a post-transplant HAP arising from the hilar hepatic arterial segment, communicating directly with the common bile duct, confirming an arterio-biliary fistula. Two sessions of transcatheter coil embolization achieved only temporary hemostasis. Following an early recurrence of bleeding, the patient underwent emergency surgery. The lesion was identified on the left hepatic artery near the porta hepatis. The surgical intervention included pseudoaneurysm resection, end-to-end hepatic artery reconstruction, and a Roux-en-Y biliary-enteric anastomosis. Despite an initially stable early postoperative course, the patient suffered a sudden circulatory collapse on postoperative day 6 and died despite aggressive resuscitation. CONCLUSIONS: Hemobilia should be considered in liver transplant recipients with recurrent or unexplained gastrointestinal bleeding, particularly when blood is observed emerging from the major duodenal papilla. DSA should be performed promptly when clinical suspicion remains high, even if post-embolization CTA is inconclusive. Recurrent bleeding after embolization may indicate persistent fistulous communication and should prompt early multidisciplinary reassessment of definitive treatment options. CI - (c) 2026 Ren, Wang, Xiong, Zhao, Wang, Lv and Ma. FAU - Ren, Qianlang AU - Ren Q AD - Yunnan Institute of Digestive Disease, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China. AD - Kunming Medical University, Kunming, Yunnan, China. FAU - Wang, Xianmei AU - Wang X AD - Yunnan Institute of Digestive Disease, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China. FAU - Xiong, Dejia AU - Xiong D AD - Yunnan Institute of Digestive Disease, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China. AD - Kunming Medical University, Kunming, Yunnan, China. FAU - Zhao, Yanxin AU - Zhao Y AD - Yunnan Institute of Digestive Disease, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China. AD - Kunming Medical University, Kunming, Yunnan, China. FAU - Wang, Guoqing AU - Wang G AD - Yunnan Institute of Digestive Disease, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China. AD - Kunming Medical University, Kunming, Yunnan, China. FAU - Lv, Junyan AU - Lv J AD - Yunnan Institute of Digestive Disease, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China. FAU - Ma, Lanqing AU - Ma L AD - Yunnan Institute of Digestive Disease, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China. LA - eng PT - Case Reports PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Med (Lausanne) JT - Frontiers in medicine JID - 101648047 PMC - PMC13709982 OTO - NOTNLM OT - arterio-biliary fistula OT - case report OT - digital subtraction angiography OT - hemobilia OT - hepatic artery pseudoaneurysm OT - liver transplantation COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/13 CRDT- 2026/10/28 04:45 PHST- 2026/07/16 00:00 [received] PHST- 2026/08/25 00:00 [revised] PHST- 2026/09/03 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:45 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fmed.2026.1940000 [doi] PST - epublish SO - Front Med (Lausanne). 2026 Oct 13;13:1940000. doi: 10.3389/fmed.2026.1940000. eCollection 2026. PMID- 42947701 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-042X (Print) IS - 1664-042X (Electronic) IS - 1664-042X (Linking) VI - 17 DP - 2026 TI - Dietary modification and nutritional supplements for exercise-induced bronchoconstriction: a systematic review and meta-analysis. PG - 1975449 LID - 10.3389/fphys.2026.1975449 [doi] LID - 1975449 AB - OBJECTIVE: Our aim was to evaluate the effects of dietary modification and nutritional supplementation on exercise-induced bronchoconstriction (EIB) and the certainty of evidence for each intervention. METHODS: PubMed, Web of Science, Scopus, and CENTRAL were searched through to 1 August 2026. Randomized parallel-group and crossover trials assessing EIB with an exercise challenge, eucapnic voluntary hyperpnea (EVH), or another indirect bronchial provocation test were included. The primary outcome was the maximum or peak percentage fall in forced expiratory volume in 1 s (FEV(1)) within 30 min, with fixed-time-point values treated as proxy outcomes. Comparable intervention-specific effects were synthesized, crossover trials were analyzed as paired designs, and Risk of Bias 2 (RoB 2) and Grading of Recommendations Assessment, Development, and Evaluation (GRADE) were applied. RESULTS: A total of 20 studies (22 reports) were included. Low sodium compared with experimentally prescribed high sodium, standard omega-3 fatty acids/fish oil, selected caffeine doses, and PCSO-524 showed potentially favorable signals. However, the evidence was limited by the small sample sizes, proxy outcomes, heterogeneity, risk of bias, missing paired data, or lack of independent replication. Adverse events were inadequately reported. CONCLUSION: Current evidence is insufficient to recommend any nutritional intervention for routine prevention or treatment of EIB. Nutritional approaches should not replace guideline-based assessment or pharmacological management. SYSTEMATIC REVIEW REGISTRATION: https://www.crd.york.ac.uk/PROSPERO/, identifier CRD420261485221. CI - Copyright (c) 2026 Su, Sun and Shan. FAU - Su, Mingxin AU - Su M AD - College of Physical Education, Central China Normal University, Wuhan, China. FAU - Sun, Richen AU - Sun R AD - School of Sports Training, Guangzhou Sport University, Guangzhou, China. FAU - Shan, Changkun AU - Shan C AD - Department of Public Health, Jinan Vocational College of Nursing, Jinan, China. LA - eng PT - Journal Article PT - Systematic Review DEP - 20261013 PL - Switzerland TA - Front Physiol JT - Frontiers in physiology JID - 101549006 PMC - PMC13710025 OTO - NOTNLM OT - dietary modification OT - exercise-induced asthma OT - exercise-induced bronchoconstriction OT - forced expiratory volume in 1 second OT - meta-analysis OT - nutritional supplementation OT - systematic review COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/13 CRDT- 2026/10/28 04:43 PHST- 2026/08/22 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:43 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fphys.2026.1975449 [doi] PST - epublish SO - Front Physiol. 2026 Oct 13;17:1975449. doi: 10.3389/fphys.2026.1975449. eCollection 2026. PMID- 42947700 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1663-9812 (Print) IS - 1663-9812 (Electronic) IS - 1663-9812 (Linking) VI - 17 DP - 2026 TI - Economic evaluation of six immune checkpoint inhibitor-based first-line therapies for advanced squamous non-small-cell lung cancer: a cost-effectiveness analysis based on network meta-analysis. PG - 1841871 LID - 10.3389/fphar.2026.1841871 [doi] LID - 1841871 AB - BACKGROUND: Advanced squamous non-small-cell lung cancer (sq-NSCLC) imposes a substantial clinical and economic burden. Immune checkpoint inhibitors (sugemalimab, tislelizumab, penpulimab, serplulimab, camrelizumab, and pembrolizumab) combined with chemotherapy improve survival, but their comparative cost-effectiveness remains uncertain. METHODS: A three-state partitioned survival model with 3-week cycles and a lifetime horizon was developed from the Chinese healthcare-system perspective. Clinical efficacy was derived from six phase 3 trials and synthesized using network meta-analysis. Costs, life-years (LYs), quality-adjusted life-years (QALYs), and incremental cost-effectiveness ratios (ICERs) for each ICI-based regimen versus chemotherapy were estimated. The willingness-to-pay (WTP) threshold was set at $27,906/QALY. Deterministic, probabilistic, scenario, and full incremental analyses were performed. RESULTS: In the base-case analysis, chemotherapy alone yielded 0.71 QALYs at $12,227.47. All ICI-based regimens increased QALYs to between 1.24 and 2.07. Pembrolizumab plus chemotherapy provided the greatest health benefit, yielding 2.07 QALYs, but had an ICER of $70,913.04 per QALY versus chemotherapy alone. Penpulimab plus chemotherapy had the lowest ICER versus chemotherapy alone ($13,064.23 per QALY), followed by camrelizumab ($13,361.50/QALY), and tislelizumab plus chemotherapy ($14,022.95/QALY), all below the WTP threshold. Sugemalimab plus chemotherapy and serplulimab plus chemotherapy exceeded the threshold. Probabilistic sensitivity analysis indicated that camrelizumab plus chemotherapy had the highest probability of being cost-effective (95%) at $27,906/QALY. In the full incremental analysis, the efficiency frontier comprised chemotherapy alone, penpulimab plus chemotherapy, camrelizumab plus chemotherapy, and pembrolizumab plus chemotherapy; camrelizumab plus chemotherapy was the preferred strategy after dominated options were excluded. CONCLUSION: At the selected WTP threshold, camrelizumab plus chemotherapy may be the preferred first-line strategy after strictly and extendedly dominated alternatives were excluded. This finding depends on the indirect comparisons, chemotherapy baseline, drug prices, survival extrapolation, and post-progression assumptions. CI - Copyright (c) 2026 Qiu, Wang, Lou and Xuan. FAU - Qiu, Zijian AU - Qiu Z AD - Department of Radiation Oncology, The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital, Quzhou, China. FAU - Wang, Guodong AU - Wang G AD - Department of Radiation Oncology, Zhuji Affiliated Hospital of Wenzhou Medical University, Shaoxing, China. FAU - Lou, Ying AU - Lou Y AD - Department of Medical Oncology, Zhuji Affiliated Hospital of Wenzhou Medical University, Shaoxing, China. FAU - Xuan, Feng AU - Xuan F AD - Department of Radiation Oncology, Zhuji Affiliated Hospital of Wenzhou Medical University, Shaoxing, China. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Pharmacol JT - Frontiers in pharmacology JID - 101548923 PMC - PMC13710048 OTO - NOTNLM OT - cost-effectiveness analysis OT - first-line therapy OT - immune checkpoint inhibitors OT - network meta-analysis OT - non-small cell lung cancer OT - partitioned survival model COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/13 CRDT- 2026/10/28 04:42 PHST- 2026/03/29 00:00 [received] PHST- 2026/08/09 00:00 [revised] PHST- 2026/09/11 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:42 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 1841871 [pii] AID - 10.3389/fphar.2026.1841871 [doi] PST - epublish SO - Front Pharmacol. 2026 Oct 13;17:1841871. doi: 10.3389/fphar.2026.1841871. eCollection 2026. PMID- 42947695 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2234-943X (Print) IS - 2234-943X (Electronic) IS - 2234-943X (Linking) VI - 16 DP - 2026 TI - A nomogram integrating ultrasound features of the primary tumor and axillary lymph nodes with clinicopathological factors to predict high axillary lymph node burden in invasive breast cancer. PG - 1906231 LID - 10.3389/fonc.2026.1906231 [doi] LID - 1906231 AB - OBJECTIVE: To develop and validate a nomogram that integrates ultrasound features of the primary tumor and axillary lymph nodes with lymphovascular invasion (LVI) for early postoperative risk stratification of high axillary nodal burden (HNB; >/=3 positive nodes) in patients with node-positive invasive breast cancer. METHODS: A total of 175 consecutive patients with node-positive invasive breast cancer who underwent surgery between July 2023, and October 2025 were retrospectively included in the development cohort, and an additional 86 patients treated during the same period comprised of the internal validation cohort. Two senior radiologists independently reviewed preoperative ultrasound images and clinicopathological data, including LVI assessed in postoperative surgical specimens. Univariate and multivariate logistic regression analyses were performed to identify independent predictors and construct the nomogram. RESULTS: The proportions of patients with posterior echo attenuation, microcalcification, claw sign, angular margin sign, spiculation sign (significant long spicules/short spicules), suspicious axillary lymph node status, and lymphovascular invasion were all higher in the high-load group than in the low-load group (all P < 0.05). Multivariate regression analysis showed that the absence of the marginal angular margin sign (OR = 0.190, 95% CI: 0.084 - 0.432, P < 0.001) and the normal axillary lymph node status (OR = 0.208, 95% CI: 0.089 - 0.456, P < 0.001) were independent protective factors for HNB, whereas lymphovascular invasion (presence) (OR = 2.707, 95% CI: 1.363 - 5.377, P = 0.004) was an independent risk factor. ROC analysis showed that the combined detection of marginal angular margin sign, lymph node status, and lymphovascular invasion had a higher AUC value for predicting HNB than individual detections (Z = 4.176, 3.781, and 3.672, respectively; P < 0.05). CONCLUSION: The nomogram incorporating the angular margin sign, axillary lymph node ultrasound status, and LVI provides effective early postoperative risk stratification for HNB in patients with node-positive invasive breast cancer and may facilitate timely adjuvant treatment decision-making. CI - Copyright (c) 2026 Li, Qin, Chen, Zhu and Yu. FAU - Li, Xiuwei AU - Li X AD - The Affiliated Lianyungang Hospital of Xuzhou Medical University/The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China. FAU - Qin, Haocheng AU - Qin H AD - The Affiliated Lianyungang Hospital of Xuzhou Medical University/The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China. FAU - Chen, Yun'an AU - Chen Y AD - The Affiliated Lianyungang Hospital of Xuzhou Medical University/The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China. FAU - Zhu, Jiale AU - Zhu J AD - The Affiliated Lianyungang Hospital of Xuzhou Medical University/The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China. FAU - Yu, Ming AU - Yu M AD - The Affiliated Lianyungang Hospital of Xuzhou Medical University/The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Oncol JT - Frontiers in oncology JID - 101568867 PMC - PMC13710060 OTO - NOTNLM OT - axillary lymph node metastasis burden OT - invasive breast cancer OT - nomogram OT - primary lesion OT - ultrasound features COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/13 CRDT- 2026/10/28 04:42 PHST- 2026/06/11 00:00 [received] PHST- 2026/08/26 00:00 [revised] PHST- 2026/09/14 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:42 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fonc.2026.1906231 [doi] PST - epublish SO - Front Oncol. 2026 Oct 13;16:1906231. doi: 10.3389/fonc.2026.1906231. eCollection 2026. PMID- 42947683 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-2392 (Print) IS - 1664-2392 (Electronic) IS - 1664-2392 (Linking) VI - 17 DP - 2026 TI - Lipid metabolism-related inflammatory indices and diabetic kidney disease in patients with type 2 diabetes mellitus: a cross-sectional study. PG - 1934556 LID - 10.3389/fendo.2026.1934556 [doi] LID - 1934556 AB - BACKGROUND: Improving the prognosis of diabetic kidney disease (DKD) requires early diagnosis and treatment. This study examined the associations of lipid metabolism-related inflammatory indices (LMIIs)-the platelet-, neutrophil-, monocyte-, and lymphocyte-to-high-density lipoprotein cholesterol ratios (PHR, NHR, MHR, and LHR, respectively)-with DKD in patients with type 2 diabetes mellitus (T2DM). METHODS: A total of 3,468 T2DM patients were enrolled in this cross-sectional study. Logistic regression models were employed to assess the association between LMIIs and DKD. Restricted cubic splines (RCS) were used to evaluate nonlinear relationships, and threshold effect analysis identified inflection points. Subgroup analyses and sensitivity analyses, including inverse probability of treatment weighting, were performed to test the robustness of the findings. Mediation analysis was conducted to explore indirect associations through selected markers. The candidate mediator was not included as a conventional covariate in the corresponding model. Receiver operating characteristic (ROC) curves were used to evaluate the discriminative performance of models incorporating the four LMIIs. RESULTS: After multivariate adjustment, all four LMIIs showed significant positive associations with DKD (P for overall < 0.05). RCS analysis revealed substantial nonlinear dose - response relationships for PHR, NHR, and MHR (P for nonlinear = 0.009, 0.014, and 0.030, respectively), with inflection points at 2.65, 4.78, and 6.07, respectively. Subgroup analyses confirmed the robustness of these associations. However, lipid-lowering agent use significantly attenuated the NHR - DKD and MHR - DKD relationships (P for interaction = 0.032 and 0.007, respectively). Sensitivity analyses yielded consistent results. TG and ALB showed statistically significant indirect associations in exploratory mediation analyses. ROC analysis showed that the model incorporating NHR had a slightly higher area under the curve (AUC) than those incorporating PHR and LHR (P = 0.002 and 0.003, respectively). CONCLUSION: LMIIs were significantly associated with DKD and exhibited distinct nonlinear dose-response relationships with threshold effects. Lipid-lowering agent use attenuated the associations of NHR and MHR with DKD. NHR may provide modestly improved discrimination compared with PHR and LHR, while showing comparable performance to MHR. These findings highlight the potential relevance of lipid-inflammation interactions in DKD and warrant further prospective studies to clarify the underlying mechanisms and clinical implications. CI - Copyright (c) 2026 Liu, Tian, Xu, Xiao, Wei, Pan, Shi, Duan, Zhang and Wei. FAU - Liu, Jing AU - Liu J AD - Department of Endocrinology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China. AD - Clinical Medical College, Beijing University of Chinese Medicine, Beijing, China. FAU - Tian, Xiaochan AU - Tian X AD - Department of Endocrinology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China. FAU - Xu, Mingjun AU - Xu M AD - Department of Geriatrics, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China. FAU - Xiao, Yao AU - Xiao Y AD - Department of Endocrinology, Fuzhou Hospital of Traditional Chinese Medicine, Fuzhou, China. FAU - Wei, Shuwu AU - Wei S AD - Department of Endocrinology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China. FAU - Pan, Xinyu AU - Pan X AD - Department of Endocrinology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China. FAU - Shi, Yue AU - Shi Y AD - Integrated Traditional Chinese and Western Medicine Diagnosis and Treatment Center, Chongqing General Hospital, Chongqing University, Chongqing, China. FAU - Duan, Hangyu AU - Duan H AD - Department of Nephrology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China. FAU - Zhang, Yu AU - Zhang Y AD - Department of Nephrology, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China. FAU - Wei, Junping AU - Wei J AD - Department of Endocrinology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Endocrinol (Lausanne) JT - Frontiers in endocrinology JID - 101555782 RN - 0 (Biomarkers) SB - IM MH - Humans MH - *Diabetes Mellitus, Type 2/complications/metabolism MH - Cross-Sectional Studies MH - Female MH - *Diabetic Nephropathies/metabolism/etiology/blood/pathology MH - Male MH - *Inflammation/metabolism MH - *Lipid Metabolism/physiology MH - Middle Aged MH - *Biomarkers/blood MH - Aged MH - Prognosis MH - Neutrophils/metabolism MH - ROC Curve PMC - PMC13709990 OTO - NOTNLM OT - diabetic kidney disease OT - high-density lipoprotein cholesterol OT - lipid metabolism-related inflammatory indices OT - lymphocytes OT - monocytes OT - neutrophils OT - platelets OT - type 2 diabetes COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/13 CRDT- 2026/10/28 04:40 PHST- 2026/07/11 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:40 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fendo.2026.1934556 [doi] PST - epublish SO - Front Endocrinol (Lausanne). 2026 Oct 13;17:1934556. doi: 10.3389/fendo.2026.1934556. eCollection 2026. PMID- 42947680 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1664-2392 (Print) IS - 1664-2392 (Electronic) IS - 1664-2392 (Linking) VI - 17 DP - 2026 TI - Personalised acromegaly treatment: implementing precision medicine in the Gulf States. PG - 1951420 LID - 10.3389/fendo.2026.1951420 [doi] LID - 1951420 AB - Acromegaly management in the Gulf region is complicated by a high burden of metabolic comorbidity, frequent presentation with macroadenomas and suboptimal biochemical control in available regional cohorts. Conventional first-generation somatostatin receptor ligands achieve biochemical control in only 30-40% of patients, rates lower than those observed in European cohorts, necessitating precision medicine strategies. MRI signal and tumour granulation patterns represent the most widely available biomarkers in Gulf healthcare systems and enable precise treatment selection. These biomarkers may help predict poor first-generation therapy response while identifying patients who may benefit from pasireotide, an efficacious second-generation somatostatin receptor ligand with multi-receptor binding capacity. Implementation of biomarker-guided therapy in Gulf healthcare systems can optimize therapeutic outcomes while addressing regional challenges of delayed diagnosis and advanced disease presentation. Regional challenges specific to Gulf healthcare systems are identified and a practical biomarker-guided algorithm tailored for the GCC region is proposed to optimise treatment selection using readily available diagnostic tools. This approach is more appropriate for Gulf populations who present with more advanced disease characteristics that may benefit from precision-guided first-line treatment selection, representing a paradigm shift away from current guideline recommendations toward truly personalised medicine. This evolution from empirical to precision medicine represents a potential advancement for improving acromegaly management where complex metabolic phenotypes demand sophisticated treatment strategies. CI - Copyright (c) 2026 Elhadd, Aldahmani, Almalki, Ekhzaimy, Almazrouei, Dabbous, Rohani and Alzahrani. FAU - Elhadd, Tarik AU - Elhadd T AD - Qatar Metabolic Institute, Department of Medicine, Hamad Medical Corporation, Doha, Qatar. FAU - Aldahmani, Khaled AU - Aldahmani K AD - Division of Endocrinology, Tawam Hospital & Sheikh Tahnoon Bin Mohammed Medical City (STMC), Al Ain, United Arab Emirates. AD - Department of Internal Medicine, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates. FAU - Almalki, Mussa AU - Almalki M AD - Obesity, Endocrine, and Metabolism Center, King Fahad Medical City, Second Health Cluster, Riyadh, Saudi Arabia. AD - College of Medicine, Alfaisal University, Riyadh, Saudi Arabia. FAU - Ekhzaimy, Aisha AU - Ekhzaimy A AD - Endocrinology & Diabetes Unit, Department of Medicine, College of Medicine and King Saud University Medical City, King Saud University, Riyadh, Saudi Arabia. FAU - Almazrouei, Raya AU - Almazrouei R AD - Division of Endocrinology, Tawam Hospital & Sheikh Tahnoon Bin Mohammed Medical City (STMC), Al Ain, United Arab Emirates. AD - Department of Internal Medicine, College of Medicine and Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates. FAU - Dabbous, Zainab AU - Dabbous Z AD - Qatar Metabolic Institute, Department of Medicine, Hamad Medical Corporation, Doha, Qatar. FAU - Rohani, Zaina AU - Rohani Z AD - Qatar Metabolic Institute, Department of Medicine, Hamad Medical Corporation, Doha, Qatar. FAU - Alzahrani, Ali AU - Alzahrani A AD - Department of Medicine, King Faisal Specialist Hospital and Research Center, Jeddah, Saudi Arabia. AD - Molecular Endocrine Section, Research and Innovation, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia. AD - Department of Medicine, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Front Endocrinol (Lausanne) JT - Frontiers in endocrinology JID - 101555782 RN - 51110-01-1 (Somatostatin) SB - IM MH - Humans MH - *Precision Medicine/methods MH - *Acromegaly/therapy/drug therapy/epidemiology MH - Somatostatin/analogs & derivatives/therapeutic use PMC - PMC13710002 OTO - NOTNLM OT - Gulf States OT - T2-weighted MRI OT - acromegaly OT - biomarkers OT - pasireotide OT - personalised therapy OT - precision medicine OT - somatostatin receptors COIS- Authors TE, ZD, and ZR are employed by the Hamad Medical Corporation, Qatar. The remaining author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/13 CRDT- 2026/10/28 04:39 PHST- 2026/07/28 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:39 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fendo.2026.1951420 [doi] PST - epublish SO - Front Endocrinol (Lausanne). 2026 Oct 13;17:1951420. doi: 10.3389/fendo.2026.1951420. eCollection 2026. PMID- 42947673 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2234-943X (Print) IS - 2234-943X (Electronic) IS - 2234-943X (Linking) VI - 16 DP - 2026 TI - Unveiling the heterogeneity: PD-L1 expression and metabolic activity in primary vs. metastatic NSCLC, a retrospective single-center analysis. PG - 1966803 LID - 10.3389/fonc.2026.1966803 [doi] LID - 1966803 AB - INTRODUCTION: Programmed death-ligand 1 (PD-L1) expression was different in primary tumors (PT) and metastatic tumors (MT). Total-body positron-emission tomography/computed tomography (PET/CT) comprehensively visualizes primary lesions and metastases. We aimed to explore the heterogeneity of PD-L1 high expression and metabolic activity between primary and metastatic lesions in patients with non-small cell lung cancer (NSCLC). METHODS: Immunohistochemistry analysis (IHC) was conducted for PD-L1 expression on tumor cells (TC) and tumor-infiltrating immune cells (IC) with 22C3 antibody. PD-L1 positive scores on TC no less than 50% or IC no less than 10% was defined as high expression. Total 598 (456 adenocarcinoma (ADC) and 142 squamous cell lung carcinoma (SCC)) primary diagnosed NSCLC patients who underwent (18)F-FDG-PET/CT imaging were collected retrospectively. The statistic methods included chi-square test, Mann Whitney test and multivariate regression. RESULTS: 598 patients included 441 with PD-L1 low expression and 157 high expression. In all subtypes and ADC, MT had higher PD-L1 high expression (P = 0.023 and P = 0.010, respectively). The maximum standard uptake value (SUVmax) of PD-L1 high expression was significantly higher than that of PD-L1 low expression, no matter in PT or MT (15.23 (8.85) vs 9.42 (8.34), P<0.001; 13.36 (5.55) vs 8.52 (6.93), P<0.001). The squamous-cell-carcinoma metastatic-lesion analyses were exploratory owing to the very small sample size and risk of type-II statistical error. Multivariate regression indicated that SUVmax was independently associated with PD-L1 high expression. In all metastatic sites, we compared the SUVmax of MT and paired PT and found SUVmax was higher in PT than MT in PD-L1 low expression subgroup (11.39 (8.12) vs 8.52 (6.93), P = 0.002). For lymph node metastases, SUVmax was significantly elevated in PT compared to MT in PD-L1 low expression (13.71 (7.95) vs 8.54 (7.30), P = 0.002), whereas no correlation was observed in PD-L1 high expression. DISCUSSION: Both PD-L1 expression and metabolism exhibited heterogeneity between primary tumors and their metastases. Compared to the primary tumors, different types of metastatic tumors showed variations not only in PD-L1 expression but also in their metabolic parameter. Utilizing whole-body PET/CT imaging facilitates the selection of optimal biopsy sites by providing metabolic reference data pertinent to clinical practice. CI - Copyright (c) 2026 Hu, Jin, Yu, Zhang, You, Fu, Xuan, Wu and Gao. FAU - Hu, Bingxin AU - Hu B AD - Department of Nuclear Medicine of Henan Provincial People's Hospital & the People's Hospital of Zhengzhou University, Zhengzhou, China. AD - Henan Key Laboratory of Novel Molecular Probes and Clinical Translation in Nuclear Medicine, Zhengzhou, China. FAU - Jin, Huibin AU - Jin H AD - Department of Nuclear Medicine of Henan Provincial People's Hospital & the People's Hospital of Zhengzhou University, Zhengzhou, China. AD - Henan Key Laboratory of Novel Molecular Probes and Clinical Translation in Nuclear Medicine, Zhengzhou, China. FAU - Yu, Yue AU - Yu Y AD - Department of Nuclear Medicine of Henan Provincial People's Hospital & the People's Hospital of Zhengzhou University, Zhengzhou, China. AD - Henan Key Laboratory of Novel Molecular Probes and Clinical Translation in Nuclear Medicine, Zhengzhou, China. FAU - Zhang, Jie AU - Zhang J AD - Department of Nuclear Medicine of Henan Provincial People's Hospital & the People's Hospital of Zhengzhou University, Zhengzhou, China. AD - Henan Key Laboratory of Novel Molecular Probes and Clinical Translation in Nuclear Medicine, Zhengzhou, China. FAU - You, Yang AU - You Y AD - Department of Nuclear Medicine of Henan Provincial People's Hospital & the People's Hospital of Zhengzhou University, Zhengzhou, China. AD - Henan Key Laboratory of Novel Molecular Probes and Clinical Translation in Nuclear Medicine, Zhengzhou, China. FAU - Fu, Chang AU - Fu C AD - Department of Nuclear Medicine of Henan Provincial People's Hospital & the People's Hospital of Zhengzhou University, Zhengzhou, China. AD - Henan Key Laboratory of Novel Molecular Probes and Clinical Translation in Nuclear Medicine, Zhengzhou, China. FAU - Xuan, Ang AU - Xuan A AD - Department of Nuclear Medicine of Henan Provincial People's Hospital & the People's Hospital of Zhengzhou University, Zhengzhou, China. AD - Henan Key Laboratory of Novel Molecular Probes and Clinical Translation in Nuclear Medicine, Zhengzhou, China. FAU - Wu, Xinyu AU - Wu X AD - Department of Nuclear Medicine of Henan Provincial People's Hospital & the People's Hospital of Zhengzhou University, Zhengzhou, China. AD - Henan Key Laboratory of Novel Molecular Probes and Clinical Translation in Nuclear Medicine, Zhengzhou, China. FAU - Gao, Yongju AU - Gao Y AD - Department of Nuclear Medicine of Henan Provincial People's Hospital & the People's Hospital of Zhengzhou University, Zhengzhou, China. AD - Henan Key Laboratory of Novel Molecular Probes and Clinical Translation in Nuclear Medicine, Zhengzhou, China. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Oncol JT - Frontiers in oncology JID - 101568867 PMC - PMC13709988 OTO - NOTNLM OT - NSCLC OT - PD-L1 OT - SUVmax OT - metastatic tumors OT - primary tumors COIS- The author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/13 CRDT- 2026/10/28 04:38 PHST- 2026/08/13 00:00 [received] PHST- 2026/09/12 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:38 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fonc.2026.1966803 [doi] PST - epublish SO - Front Oncol. 2026 Oct 13;16:1966803. doi: 10.3389/fonc.2026.1966803. eCollection 2026. PMID- 42947672 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2234-943X (Print) IS - 2234-943X (Electronic) IS - 2234-943X (Linking) VI - 16 DP - 2026 TI - Predictors of guideline-concordant treatment intensification in metastatic castration-sensitive prostate cancer: an explainable machine learning study. PG - 1835503 LID - 10.3389/fonc.2026.1835503 [doi] LID - 1835503 AB - BACKGROUND: Treatment intensification with androgen deprivation therapy (ADT) combined with docetaxel, an androgen receptor pathway inhibitor, or selected triplet regimens is recommended for eligible patients with metastatic castration-sensitive prostate cancer (mCSPC). Despite increasing adoption, implementation remains variable in routine clinical practice. OBJECTIVE: To characterize temporal trends in first-line systemic treatment for mCSPC and identify factors associated with receipt of guideline-concordant treatment intensification using exploratory explainable machine learning. METHODS: National treatment patterns were evaluated using the Pfizer Oncology Quality Care Insight Tool derived from IQVIA Anonymized Patient Longitudinal Data, and institutional patterns were evaluated within Avera Health. The exploratory cohort included 42 patients with documented mCSPC treated between 2018 and 2023. Guideline-concordant treatment intensification was defined as ADT combined with docetaxel or a novel hormonal therapy within 90 days of ADT initiation. Potential outcome-derived variables were excluded before modeling. Six predictive approaches were evaluated using repeated nested stratified cross-validation, with 5-fold outer cross-validation repeated 20 times and 5-fold inner cross-validation repeated three times. Recursive feature elimination was performed within outer training folds, and class imbalance was addressed using SMOTE within resampling. RESULTS: Thirty-two (32) of forty-two (42) patients (76.2%) received guideline-concordant treatment intensification. Treatment-pattern analysis showed increasing use of first-line novel hormonal therapy and declining ADT monotherapy over time. Under repeated nested validation, discrimination was modest and variable across models. The Decision Tree achieved the highest patient-level AUC (0.675; 95% CI, 0.421-0.929), whereas Random Forest achieved an AUC of 0.650 (95% CI, 0.383-0.917), accuracy of 85.7%, precision of 88.2%, recall of 93.8%, and F1 score of 0.909. Feature-selection stability identified insurance coverage for novel hormonal therapy as the most consistently selected predictor, followed by positive core proportion and smoking history. These three variables were retained in the final explanatory Random Forest model, and global SHAP and Random Forest importance analysis showed broadly concordant rankings. CONCLUSIONS: Treatment intensification for mCSPC increased substantially in routine practice. Repeated nested validation produced more conservative and variable performance estimates than the original single-split analysis, underscoring the exploratory nature of this small cohort. Healthcare access and selected clinical factors warrant further evaluation in larger, multicenter datasets. CI - Copyright (c) 2026 Barton Odro, Bychinski, Van Alstyne, Corsini, Owen, Jones, Krebs, Solomon, Vaca, Williams, Elsey and Meissner. FAU - Barton Odro, Emmanuel AU - Barton Odro E AD - Avera Cancer Institute, Sioux Falls, SD, United States. FAU - Bychinski, James AU - Bychinski J AD - Pfizer Inc, Groton, NY, United States. FAU - Van Alstyne, Ted AU - Van Alstyne T AD - University of South Dakota, Sanford School of Medicine, Sioux Falls, SD, United States. FAU - Corsini, Connor AU - Corsini C AD - University of South Dakota, Sanford School of Medicine, Sioux Falls, SD, United States. FAU - Owen, Parker AU - Owen P AD - University of South Dakota, Sanford School of Medicine, Sioux Falls, SD, United States. FAU - Jones, Cheryl Page AU - Jones CP AD - Avera Cancer Institute, Sioux Falls, SD, United States. FAU - Krebs, Nicole AU - Krebs N AD - Avera Cancer Institute, Sioux Falls, SD, United States. FAU - Solomon, Benjamin AU - Solomon B AD - Avera Cancer Institute, Sioux Falls, SD, United States. FAU - Vaca, Ryan AU - Vaca R AD - Avera Cancer Institute, Sioux Falls, SD, United States. FAU - Williams, Casey AU - Williams C AD - Avera Cancer Institute, Sioux Falls, SD, United States. FAU - Elsey, Rachel AU - Elsey R AD - Avera Cancer Institute, Sioux Falls, SD, United States. FAU - Meissner, Tobias AU - Meissner T AD - Avera Cancer Institute, Sioux Falls, SD, United States. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Front Oncol JT - Frontiers in oncology JID - 101568867 PMC - PMC13710003 OTO - NOTNLM OT - SHAP OT - guideline-concordant care OT - healthcare access OT - machine learning OT - metastatic castration-sensitive prostate cancer OT - novel hormonal therapy OT - real-world evidence OT - treatment intensification COIS- Author James Bychinski was employed by Pfizer Inc. The remaining author(s) declared that this work was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/13 CRDT- 2026/10/28 04:38 PHST- 2026/03/20 00:00 [received] PHST- 2026/08/09 00:00 [revised] PHST- 2026/08/22 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:38 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - 10.3389/fonc.2026.1835503 [doi] PST - epublish SO - Front Oncol. 2026 Oct 13;16:1835503. doi: 10.3389/fonc.2026.1835503. eCollection 2026. PMID- 42947666 OWN - NLM STAT- Publisher LR - 20261028 IS - 1528-0691 (Electronic) IS - 1528-0691 (Linking) DP - 2026 Oct 28 TI - Metal-Catalyzed Benzannulation: Recent Advances in Catalyst Design, Mechanistic Insights, and Aromatic Framework Construction. LID - e70265 [pii] LID - 10.1002/tcr.70265 [doi] AB - Benzannulation is a key transformation in organic synthesis, enabling direct access to aromatic and heteroaromatic scaffolds central to natural products, pharmaceuticals, agrochemicals, dyes, electronic devices, and materials. The construction of these frameworks continues to pose a challenge because it requires high regioselectivity, broad substrate scope, and functional-group tolerance. Traditional multistep approaches often suffer from limited efficiency, highlighting the need for more versatile synthetic strategies. Metal-catalyzed benzannulation has emerged as an effective solution, offering excellent efficiency, high selectivity, and broad synthetic applicability. Catalysts based on transition metals (Sc, Fe, Co, Ni, Cu, Ru, Rh, Pd, Ag, Ir, and Au), together with main-group, post-transition, and rare-earth metals (Ca, Al, In, Bi, La, and Yb), have expanded the scope of these transformations. Despite these advances, a comprehensive understanding of catalyst design, reaction mechanisms, and synthetic applications remains incomplete, underscoring the need for an up-to-date and systematic overview. This review addresses this need by providing an overview of metal-catalyzed benzannulation reported since 2018, with particular emphasis on catalyst design, mechanistic insights, and synthetic applications. By integrating recent advances across diverse metal-catalyzed systems, this review provides a comprehensive perspective on the field and offers guidance for the future development of efficient and selective benzannulation methodologies. CI - (c) 2026 The Author(s). The Chemical Record published by The Chemical Society of Japan and Wiley-VCH GmbH. FAU - Jamshaid, Sana AU - Jamshaid S AUID- ORCID: 0000-0003-3804-5960 AD - Department of Chemistry, Hanyang University, Seoul, Republic of Korea. FAU - Shrestha, Divya AU - Shrestha D AUID- ORCID: 0009-0006-1789-5930 AD - School of Chemical Engineering, Yeungnam University, Gyeongsan, Republic of Korea. FAU - Thombal, Raju S AU - Thombal RS AUID- ORCID: 0000-0003-3760-8856 AD - School of Chemical Engineering, Yeungnam University, Gyeongsan, Republic of Korea. FAU - Kim, Jeung Gon AU - Kim JG AUID- ORCID: 0000-0003-1685-2833 AD - Department of Chemistry, Hanyang University, Seoul, Republic of Korea. FAU - Cheol, Kim Seong AU - Cheol KS AUID- ORCID: 0000-0001-8028-8320 AD - School of Chemical Engineering, Yeungnam University, Gyeongsan, Republic of Korea. FAU - Lee, Yong Rok AU - Lee YR AUID- ORCID: 0000-0002-4048-8341 AD - School of Chemical Engineering, Yeungnam University, Gyeongsan, Republic of Korea. LA - eng GR - RS-2025-00556748/National Research Foundation of Korea/ PT - Journal Article PT - Review DEP - 20261028 PL - United States TA - Chem Rec JT - Chemical record (New York, N.Y.) JID - 101085550 SB - IM OTO - NOTNLM OT - aromatic compounds OT - benzannulation OT - catalysis OT - cyclization reactions OT - transition-metal catalysis EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 04:34 PHST- 2026/09/28 00:00 [revised] PHST- 2026/08/05 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:34 [entrez] AID - e70265 [pii] AID - 10.1002/tcr.70265 [doi] PST - aheadofprint SO - Chem Rec. 2026 Oct 28:e70265. doi: 10.1002/tcr.70265. PMID- 42947653 OWN - NLM STAT- Publisher LR - 20261028 IS - 1473-5628 (Electronic) IS - 0143-3636 (Linking) DP - 2026 Oct 28 TI - 18F-FDG PET/MRI-based liver and bone marrow organomics combined with systemic inflammatory response for predicting pathological complete response after neoadjuvant therapy in breast cancer. LID - 10.1097/MNM.0000000000002266 [doi] AB - OBJECTIVE: To evaluate the role of liver and bone marrow organomics features derived from staging 18F-fluorodeoxyglucose (18F-FDG) PET/MRI in predicting pathological complete response (pCR) in breast cancer patients who underwent surgery after neoadjuvant therapy. METHODS: In this retrospective study, breast cancer patients who underwent pretreatment staging with 18F-FDG PET/MRI, received neoadjuvant therapy, and subsequently underwent surgery were included. Clinicopathological characteristics and systemic inflammatory markers were recorded. Organomics features were extracted from the liver and L4 vertebra bone marrow regions on 18F-FDG PET images using LIFEx software. Least Absolute Shrinkage and Selection Operator regression was applied for radiomics feature selection, and a radiomics score (Radscore) was generated. Logistic regression analyses were performed to identify predictors of pCR. The diagnostic performances of the Radscore, Clinical, and Clinical-Radscore Combined models were evaluated using receiver operating characteristic analysis. RESULTS: A total of 87 breast cancer patients were included, and pCR was observed in 33 patients (37.9%). In multivariable analysis, progesterone receptor status, human epidermal growth factor receptor 2 status, primary tumor size, and Radscore were identified as independent predictors of pCR. The Radscore yielded an area under the curve (AUC) of 0.722 [95% confidence interval (CI) = 0.625-0.819], whereas the Clinical and Clinical-Radscore Combined models achieved AUCs of 0.850 (95% CI = 0.765-0.934) and 0.904 (95% CI = 0.843-0.965), respectively. The Clinical-Radscore Combined Model demonstrated higher diagnostic performance than both the other models. CONCLUSION: Liver and bone marrow organomics features derived from staging 18F-FDG PET/MRI may provide valuable information for predicting pCR in breast cancer patients undergoing neoadjuvant therapy. CI - Copyright (c) 2026 Wolters Kluwer Health, LLC. All rights reserved. FAU - Gulbahar Ates, Seda AU - Gulbahar Ates S AUID- ORCID: 0000-0003-0422-0863 AD - Department of Nuclear Medicine, Gazi University Faculty of Medicine, Ankara, Turkey. FAU - Bozyigit, Merve Nur AU - Bozyigit MN AUID- ORCID: 0009-0006-0897-4725 FAU - Aydos, Uguray AU - Aydos U AUID- ORCID: 0000-0003-0582-5020 FAU - Atay, Lutfiye Ozlem AU - Atay LO AUID- ORCID: 0000-0003-3154-656 LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Nucl Med Commun JT - Nuclear medicine communications JID - 8201017 SB - IM OTO - NOTNLM OT - breast cancer OT - organomics OT - pathological complete response OT - radiomics EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 04:14 PHST- 2026/05/21 00:00 [received] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:14 [entrez] AID - 00006231-990000000-00672 [pii] AID - 10.1097/MNM.0000000000002266 [doi] PST - aheadofprint SO - Nucl Med Commun. 2026 Oct 28. doi: 10.1097/MNM.0000000000002266. PMID- 42947645 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1520-636X (Electronic) IS - 0899-0042 (Linking) VI - 38 IP - 11 DP - 2026 Nov TI - Chiral Nanomaterials for Antiviral Applications, From Direct Viral Inactivation to Immune Modulation. LID - e70159 [pii] LID - 10.1002/chir.70159 [doi] AB - Viral infections persistently threaten public health, and chirality offers a distinctive dimension for the design of antiviral materials. Recently, a series of advances in the use of chiral nanomaterials against viruses, which fall into two major categories. The first is direct antiviral action through chiral recognition, including viral inhibition, agglutination, inactivation, and site-selective cleavage. The second is the modulation of antiviral immunity by chiral nanoadjuvants and nanovaccines. This minireview examines representative recent work along these two lines and discusses the scientific value of each study. Building on this analysis, we present a critical perspective on the common problems of the field. Chiral inorganic nanomaterials lack a defined molecular composition, exhibit pronounced batch-to-batch and within-batch heterogeneity, and possess no metric of chiral strength beyond the ensemble-averaged circular dichroism signal. They therefore fail the fundamental requirement of material certainty in drug development, and their prospects as antiviral drugs are unpromising. Chiral molecular material adjuvants and the chiral retrofitting of aluminum adjuvants embedded in established regulatory frameworks represent relatively feasible translational paths, whereas the more realistic outlet for chiral inorganic nanomaterials lies in active protective equipment, environmental filtration, and agricultural virus control, scenarios in which the material is far less likely to enter human circulation. CI - (c) 2026 Wiley Periodicals LLC. FAU - Meng, Dan AU - Meng D AD - School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, China. AD - Key Laboratory of Organosilicon Chemistry and Material Technology, Ministry of Education, Zhejiang Key Laboratory of Organosilicon Material Technology, College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, Zhejiang, China. FAU - Gao, Rui AU - Gao R AUID- ORCID: 0009-0003-2192-2303 AD - Zhejiang Provincial Key Laboratory of Pancreatic Disease, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. LA - eng GR - 22575209/National Natural Science Foundation of China/ GR - 22307046/National Natural Science Foundation of China/ GR - 22504026/National Natural Science Foundation of China/ GR - LMS26B050001/Zhejiang Provincial Natural Science Foundation of China/ PT - Journal Article PT - Review PL - United States TA - Chirality JT - Chirality JID - 8914261 RN - 0 (Antiviral Agents) SB - IM MH - *Antiviral Agents/chemistry/pharmacology MH - *Nanostructures/chemistry MH - Humans MH - *Virus Inactivation/drug effects MH - Stereoisomerism MH - *Viruses/drug effects MH - Animals OTO - NOTNLM OT - antiviral OT - chiral nanomaterials OT - nanoadjuvant OT - translational medicine EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 04:13 PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/07 00:00 [received] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:13 [entrez] AID - e70159 [pii] AID - 10.1002/chir.70159 [doi] PST - ppublish SO - Chirality. 2026 Nov;38(11):e70159. doi: 10.1002/chir.70159. PMID- 42947625 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1938-744X (Electronic) IS - 1935-7893 (Linking) VI - 20 DP - 2026 Oct 28 TI - Alternate Care Facilities Symposium-Breakout Session 6: Site Selection. PG - e200 LID - 10.1017/dmp.2026.10382 [doi] AB - The use of alternate care facilities (ACFs) is an often-considered mechanism to increase care capacity during a patient surge event; however, robust guidance on appropriate site selection is limited. During the Alternate Care Facilities Symposium, we conducted a facilitated breakout session with participants to explore their experience in selecting ACF sites during prior emergencies. This was done to determine best practices regarding the site selection process, as well as successes, challenges, and lessons learned. Participants reported that site feasibility was primarily driven by pre-existing infrastructure, regulatory constraints, and operational factors rather than physical size or political mandate. Large congregate spaces and decommissioned hospitals frequently proved unsuitable, while successful sites were typically non-congregate, located near partner hospitals, and able to support patient dignity, safety, and privacy. Political pressures often resulted in facilities that were underutilized or operationally constrained, while infrastructure deficiencies created major barriers. Given that site selection is a critical determinant of ACF success, early multidisciplinary assessments, realistic capacity planning, and pre-activation walkthroughs and training exercises may improve future emergency response preparedness. FAU - Shetty, Pranav AU - Shetty P AUID- ORCID: 0000-0002-4026-8488 AD - University of California, Davis, School of Medicine, Sacramento, CA, USA. ROR: https://ror.org/05rrcem69 FAU - Buettner, David AU - Buettner D AD - University of California, Davis, School of Medicine, Sacramento, CA, USA. ROR: https://ror.org/05rrcem69 FAU - Huffman, Elaina AU - Huffman E AD - All Clear Emergency Management Group, LLC, USA. FAU - Herron, Amy Katherine AU - Herron AK AD - All Clear Emergency Management Group, LLC, USA. LA - eng GR - MTEC-23-09-NDMS-Modular-005/Medical Technology Enterprise Consortium/ PT - Journal Article DEP - 20261028 PL - United States TA - Disaster Med Public Health Prep JT - Disaster medicine and public health preparedness JID - 101297401 SB - IM MH - Humans MH - *Disaster Planning/methods MH - Congresses as Topic EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 04:03 PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:03 [entrez] AID - S1935789326103826 [pii] AID - 10.1017/dmp.2026.10382 [doi] PST - epublish SO - Disaster Med Public Health Prep. 2026 Oct 28;20:e200. doi: 10.1017/dmp.2026.10382. PMID- 42947623 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1938-744X (Electronic) IS - 1935-7893 (Linking) VI - 20 DP - 2026 Oct 28 TI - Breakout Session 1: Patient Selection Criteria. PG - e199 LID - 10.1017/dmp.2026.10383 [doi] AB - This summary focuses on findings from the Patient Selection Breakout Session of the Alternate Care Facility (ACF) Symposium. Findings demonstrate that patient selection criteria were primarily shaped by community needs and operational constraints rather than clinical factors alone. Participants described using local population needs as a starting point to define ACF eligibility. Resource availability emerged as the dominant determinant of admission criteria, often superseding traditional clinical thresholds. External pressures from hospital systems frequently drove expansion of criteria to include higher-acuity patients requiring balancing of resources and capabilities. Successful selection criteria were characterized by iterative, multidisciplinary reviews incorporating frontline staff and strong interagency collaboration. Key challenges included clinician hesitancy to transfer patients to the ACF, limited external communication regarding capabilities, and persistent tension between flexible criteria and standardized guidance for dissemination. Additional barriers included staffing limitations, facility design constraints, and unanticipated logistical factors. External influences, including political decision-making, at times disrupted operational alignment with frontline realities. Patient selection represents a dynamic, resource-driven process requiring continuous reassessment. These findings support the need for flexible, resource-informed selection criteria, coordination with hospital partners, incorporation of frontline expertise into decision-making, and integration of discharge planning services to expand appropriate patient populations while maintaining quality care. FAU - Ryder, Jessica Ashley AU - Ryder JA AUID- ORCID: 0009-0007-3870-4395 AD - Emergency Medicine, University of Colorado Anschutz Medical Campus School of Medicine, Aurora, Colorado, USA. ROR: https://ror.org/03wmf1y16 FAU - Herron, Amy Katherine AU - Herron AK AD - All Clear Emergency Management Group, LLC, Raleigh, North Carolina, USA. FAU - Huffman, Elaina AU - Huffman E AD - All Clear Emergency Management Group, LLC, Raleigh, North Carolina, USA. LA - eng GR - METC-23-09-NDMS-Modular Number W81XWH-15-9-0001/Medical Technology Enterprise Consortium/ PT - Journal Article DEP - 20261028 PL - United States TA - Disaster Med Public Health Prep JT - Disaster medicine and public health preparedness JID - 101297401 SB - IM MH - Humans MH - *Patient Selection OTO - NOTNLM OT - Alternate Care Facility OT - Alternate Care Site OT - patient exclusion OT - patient selection EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 04:03 PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 04:03 [entrez] AID - S1935789326103838 [pii] AID - 10.1017/dmp.2026.10383 [doi] PST - epublish SO - Disaster Med Public Health Prep. 2026 Oct 28;20:e199. doi: 10.1017/dmp.2026.10383. PMID- 42947610 OWN - NLM STAT- Publisher LR - 20261028 IS - 2192-2659 (Electronic) IS - 2192-2640 (Linking) DP - 2026 Oct 28 TI - Modeling Early Metastatic Progression in Breast Cancer Using a Barrier-Free, Perfusable Spheroid-on-a-Chip Platform. LID - e71910 [pii] LID - 10.1002/adhm.71910 [doi] AB - Metastatic dissemination is the leading cause of cancer-related mortality, yet the early steps in this process remain difficult to study using current experimental models. Although organ-on-a-chip platforms recapitulate key features of the tumor microenvironment (TME), most fail to integrate physiologically relevant invasion, intravasation, and circulation within a single system. Membrane- and pillar-based designs hinder endothelial crossing and imaging, while open-interface approaches often lack stable perfusion and controlled positioning of 3D cell aggregates. Here, an open-port, barrier-free microfluidic device is presented, fabricated via surface treatment-based patterning, for investigating early metastatic progression from a tumor spheroid. The planar device incorporates a centrally confined gel matrix accessible via a top-loading port for precise spheroid placement, flanked by endothelialized lateral channels in direct contact with the matrix and perfusable under defined shear stress. Using MDA-MB-231 breast cancer spheroids, the model recapitulates key aspects of tumor cell migration, tumor-induced endothelial disruption, and intravasation, while enabling flow-mediated downstream recovery of viable intravasated cells. Overall, this device provides a physiologically relevant platform for the integrated, high-resolution, and longitudinal investigation of early metastatic events, providing a foundation for future mechanistic studies and molecular characterization of metastatic cell populations. CI - (c) 2026 The Author(s). Advanced Healthcare Materials published by Wiley-VCH GmbH. FAU - Sharko, Vira AU - Sharko V AUID- ORCID: 0009-0008-0679-8451 AD - Tissue Microenvironment (TME) Lab, Aragon Institute of Engineering Research (I3A), University of Zaragoza, Zaragoza, Spain. AD - Institute for Health Research Aragon (IIS Aragon), Zaragoza, Spain. FAU - Olaizola-Rodrigo, Claudia AU - Olaizola-Rodrigo C AUID- ORCID: 0000-0003-2701-3197 AD - Tissue Microenvironment (TME) Lab, Aragon Institute of Engineering Research (I3A), University of Zaragoza, Zaragoza, Spain. AD - Beonchip S.L., Zaragoza, Spain. FAU - Bayona, Clara AU - Bayona C AUID- ORCID: 0000-0001-6786-2041 AD - Tissue Microenvironment (TME) Lab, Aragon Institute of Engineering Research (I3A), University of Zaragoza, Zaragoza, Spain. AD - Institute for Health Research Aragon (IIS Aragon), Zaragoza, Spain. FAU - Randelovic, Teodora AU - Randelovic T AUID- ORCID: 0000-0001-7232-7588 AD - Tissue Microenvironment (TME) Lab, Aragon Institute of Engineering Research (I3A), University of Zaragoza, Zaragoza, Spain. AD - Institute for Health Research Aragon (IIS Aragon), Zaragoza, Spain. AD - CIBER-BBN - ISCIII, Zaragoza, Spain. FAU - Monge, Rosa AU - Monge R AUID- ORCID: 0000-0003-0734-129X AD - Beonchip S.L., Zaragoza, Spain. FAU - Solanas, Estela AU - Solanas E AUID- ORCID: 0000-0001-8056-5236 AD - Tissue Microenvironment (TME) Lab, Aragon Institute of Engineering Research (I3A), University of Zaragoza, Zaragoza, Spain. AD - Institute for Health Research Aragon (IIS Aragon), Zaragoza, Spain. FAU - Olivan, Sara AU - Olivan S AUID- ORCID: 0000-0003-0156-4230 AD - Tissue Microenvironment (TME) Lab, Aragon Institute of Engineering Research (I3A), University of Zaragoza, Zaragoza, Spain. AD - Institute for Health Research Aragon (IIS Aragon), Zaragoza, Spain. AD - CIBER-BBN - ISCIII, Zaragoza, Spain. FAU - Ochoa, Ignacio AU - Ochoa I AUID- ORCID: 0000-0003-2410-5678 AD - Tissue Microenvironment (TME) Lab, Aragon Institute of Engineering Research (I3A), University of Zaragoza, Zaragoza, Spain. AD - Institute for Health Research Aragon (IIS Aragon), Zaragoza, Spain. AD - CIBER-BBN - ISCIII, Zaragoza, Spain. LA - eng GR - GA 101140192-2/Chips Joint Undertaking/ GR - DIN 2020-011544/Ministerio de Economia y Competitividad (MINECO)/ GR - PREDOCDGA20/CPB_35;PREDOCDGA21/01;T62_23R/Diputacion General de Aragon (DGA)/ GR - PID2021-126051OB-C41/MCIN /AEI /10.13039/501100011033/ FEDER, UE/ PT - Journal Article DEP - 20261028 PL - Germany TA - Adv Healthc Mater JT - Advanced healthcare materials JID - 101581613 SB - IM OTO - NOTNLM OT - 3D culture OT - barrier-free model OT - intravasation OT - metastasis OT - microfluidic perfusion OT - organ-on-a-chip OT - spheroid-on-a-chip EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 03:53 PHST- 2026/10/14 00:00 [revised] PHST- 2026/03/06 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 03:53 [entrez] AID - e71910 [pii] AID - 10.1002/adhm.71910 [doi] PST - aheadofprint SO - Adv Healthc Mater. 2026 Oct 28:e71910. doi: 10.1002/adhm.71910. PMID- 42947606 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1097-0258 (Electronic) IS - 0277-6715 (Linking) VI - 45 IP - 25-27 DP - 2026 Nov TI - Online Updating Mixture Cure Models for Large-Scale Survival Data Streams. LID - e70795 [pii] LID - 10.1002/sim.70795 [doi] AB - The growing availability of large-scale survival data streams present significant challenges for statistical inference particularly in the presence of a cure fraction, which complicates estimation and increases computational burden. To address these challenges, we propose an online updating mixture cure model framework. Our approach employs a batchwise renewable estimation procedure that aggregates historical information into summary statistics to update the model without retaining historical individual-level data, and thus eliminating the need for full data storage. A renewable penalized expectation-maximization algorithm is developed to enable simultaneous parameter estimation and variable selection. Furthermore, a tuning-free strategy is incorporated to enhance computational efficiency. The asymptotic properties of the proposed method are established. Simulation studies and an application to breast cancer data from the Surveillance, Epidemiology, and End Results database demonstrate the method's estimation accuracy, predictive performance, and computational scalability. CI - (c) 2026 John Wiley & Sons Ltd. FAU - Jin, Li AU - Jin L AUID- ORCID: 0000-0003-4122-4943 AD - Department of Statistics and Data Science, School of Management, Fudan University, Shanghai, P. R. China. AD - School of Mathematical Sciences, Capital Normal University, Beijing, P. R. China. FAU - Hu, Tao AU - Hu T AUID- ORCID: 0000-0003-4463-004X AD - School of Mathematical Sciences, Capital Normal University, Beijing, P. R. China. FAU - Xiang, Liming AU - Xiang L AUID- ORCID: 0000-0003-0698-5173 AD - School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore, Singapore. LA - eng GR - JWZQ20240101027/Beijing Outstanding Young Scientist Program/ GR - RG105/24/Singapore Ministry of Education Academic Research Fund Tier 1 Grant/ GR - 12671349/National Natural Science Foundation of China/ PT - Journal Article PL - England TA - Stat Med JT - Statistics in medicine JID - 8215016 SB - IM MH - Humans MH - Breast Neoplasms/mortality MH - Algorithms MH - Computer Simulation MH - Survival Analysis MH - *Models, Statistical MH - SEER Program MH - Female OTO - NOTNLM OT - EM algorithm OT - mixture cure model OT - online learning OT - streaming survival data OT - variable selection EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 03:43 PHST- 2026/09/25 00:00 [revised] PHST- 2026/04/15 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 03:43 [entrez] AID - e70795 [pii] AID - 10.1002/sim.70795 [doi] PST - ppublish SO - Stat Med. 2026 Nov;45(25-27):e70795. doi: 10.1002/sim.70795. PMID- 42947604 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1097-4652 (Electronic) IS - 0021-9541 (Linking) VI - 241 IP - 10 DP - 2026 Oct TI - Chronic Stress Promotes Lung Adenocarcinoma Progression via alpha5-nAChR/FOXM1 Signaling Pathway. LID - e70250 [pii] LID - 10.1002/jcp.70250 [doi] AB - The alpha5-nicotinic acetylcholine receptor (alpha5-nAChR) mediates chronic stress-induced lung adenocarcinoma (LUAD) progression. Forkhead Box M1 (FOXM1) is an oxidative stress-associated protein that is strongly associated with chronic stress and functions as a cancer-promoting transcription factor critical to LUAD. Our gene expression profiling demonstrated that CHRNA5 (encoding alpha5-nAChR) knockdown suppresses FOXM1 levels. It remains unknown whether and how alpha5-nAChR interacts with FOXM1 to promote chronic stress-induced LUAD progression. In this study, the expression and correlation of alpha5-nAChR and FOXM1 in LUAD of mice were investigated through chronic restraint stress (CRS). alpha5-nAChR expression was related to FOXM1 expression, low survival rate, staging, and smoking status in both LUAD dataset and tissue microarrays. In vitro experiments demonstrated that alpha5-nAChR mediates acetylcholine (ACh)-induced FOXM1, Ki67, and Sox2 expression via STAT3. alpha5-nAChR/FOXM1 signaling contributes to LUAD cell proliferation, migration, and invasion. Furthermore, alpha5-nAChR expression was linked to FOXM1 expression in chronic unpredictable mild stress (CUMS) mice tissues. The findings suggested that alpha5-nAChR/FOXM1 axis is involved in chronic stress-promoted LUAD progression. CI - (c) 2026 Wiley Periodicals LLC. FAU - Cai, Jiaying AU - Cai J AD - Center for Basic and translational Medical Research, Central Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. FAU - Wang, Zepeng AU - Wang Z AUID- ORCID: 0009-0000-6080-7285 AD - Center for Basic and translational Medical Research, Central Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. FAU - Lu, Tong AU - Lu T AD - Center for Basic and translational Medical Research, Central Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. FAU - Wang, Zengping AU - Wang Z AD - Department of Medical Laboratory, Shandong Second Medical University, Weifang, Shandong, China. FAU - Yang, Mengdan AU - Yang M AD - Department of Medical Laboratory, Shandong Second Medical University, Weifang, Shandong, China. FAU - Yang, Shuran AU - Yang S AD - Center for Basic and translational Medical Research, Central Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. FAU - Zhao, Wendi AU - Zhao W AD - Department of Medical Laboratory, Shandong Second Medical University, Weifang, Shandong, China. FAU - Jia, Yanfei AU - Jia Y AUID- ORCID: 0000-0002-0670-1604 AD - Center for Basic and translational Medical Research, Central Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. FAU - Sun, Haiji AU - Sun H AD - College of Life Science, Shandong Normal University, Jinan, Shandong, China. FAU - Ma, Xiaoli AU - Ma X AUID- ORCID: 0000-0003-0279-8354 AD - Center for Basic and translational Medical Research, Central Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China. LA - eng GR - ZR2025MS1438/Natural Science Foundation of Shandong Province/ GR - 202304110747/Shandong Provincial Medical and Health Research Project/ GR - 202511000793/Shandong Provincial Medical and Health Research Project/ PT - Journal Article PL - United States TA - J Cell Physiol JT - Journal of cellular physiology JID - 0050222 RN - 0 (Forkhead Box Protein M1) RN - 0 (Receptors, Nicotinic) RN - 0 (Foxm1 protein, mouse) RN - 0 (FOXM1 protein, human) SB - IM MH - *Forkhead Box Protein M1/metabolism/genetics MH - Animals MH - Signal Transduction MH - *Adenocarcinoma of Lung/pathology/metabolism/genetics MH - Disease Progression MH - *Lung Neoplasms/pathology/metabolism/genetics MH - Cell Proliferation MH - *Receptors, Nicotinic/metabolism/genetics MH - Humans MH - Male MH - Mice MH - Gene Expression Regulation, Neoplastic MH - Cell Movement MH - Cell Line, Tumor OTO - NOTNLM OT - FOXM1 OT - acetylcholine OT - chronic stress OT - lung adenocarcinoma OT - alpha5-nAChR EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 03:43 PHST- 2026/07/23 00:00 [revised] PHST- 2026/05/14 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 03:43 [entrez] AID - e70250 [pii] AID - 10.1002/jcp.70250 [doi] PST - ppublish SO - J Cell Physiol. 2026 Oct;241(10):e70250. doi: 10.1002/jcp.70250. PMID- 42947603 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261028 IS - 1738-1088 (Print) IS - 1738-1088 (Linking) VI - 24 IP - 4 DP - 2026 Nov 30 TI - Ekbom Spectrum Psychosis Associated with 24-hour Continuous Subcutaneous Foslevodopa/Foscarbidopa Infusion in Parkinson's Disease. PG - 885-889 LID - 10.9758/cpn.26.1449 [doi] AB - Continuous subcutaneous foslevodopa/foscarbidopa infusion (CSFLI) is an emerging treatment for advanced Parkinson's disease (PD). We report atypical psychosis with Ekbom-like somatic hallucinations during CSFLI. A 74-year-old man with a 16-year history of PD (Hoehn and Yahr stage 3), mild cognitive impairment (Montreal Cognitive Assessment 25/30), and previous mild dopamine agonist-induced visual hallucinations received CSFLI for disabling motor fluctuations, dyskinesias, nocturnal rigidity, and severe morning akinesia inadequately controlled by oral therapy. Initial treatment was well tolerated, reducing OFF time, dyskinesias, and sleep disturbance. Because of recurrent motor fluctuations and nocturnal akinesia, infusion parameters were progressively increased to a daytime base rate of 0.48 ml/h and a nocturnal low-rate of 0.38 ml/h, corresponding to an estimated levodopa equivalent daily dose of approximately 1,868 mg/day. Seventy-two hours after ciprofloxacin initiation for an infected subcutaneous nodule, the patient developed vivid visual hallucinations that evolved into Ekbom-like somatic hallucinations, described as worms moving beneath the skin, leading to superficial self-inflicted lacerations. Orientation remained preserved and no delirium features were observed. Initial reduction of infusion parameters, safinamide discontinuation, and quetiapine produced only partial benefit. A subsequent selective reduction of the nocturnal low-rate from 0.36 to 0.30 ml/h was associated with a clinically meaningful reduction in psychotic symptoms, although residual hallucinations and worsening morning akinesia persisted. This case suggests that psychosis during CSFLI may reflect the interaction between neuropsychiatric vulnerability, ciprofloxacin exposure, and sustained nocturnal dopaminergic stimulation. Although causality cannot be inferred, careful nocturnal titration may help clinicians recognize and manage atypical psychotic manifestations early in susceptible patients. FAU - Meloni, Mario AU - Meloni M AUID- ORCID: 0000-0002-6319-3458 AD - Neurology Unit, University Hospital of Cagliari, Cagliari, Italy. FAU - Ascaniis, Fabrizio De AU - Ascaniis F AUID- ORCID: 0009-0003-8256-1476 AD - Department of Medical Science and Public Health, University of Cagliari, Cagliari, Italy. FAU - Orofino, Gianni AU - Orofino G AUID- ORCID: 0009-0000-8135-8466 AD - Neurology Unit, University Hospital of Cagliari, Cagliari, Italy. FAU - Marano, Massimo AU - Marano M AUID- ORCID: 0000-0001-9885-3666 AD - Unit of Neurology, Neurophysiology, Neurobiology and Psychiatry, Department of Medicine, Universita Campus Bio-Medico di Roma, Rome, Italy. AD - Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy. FAU - Mulas, Martina AU - Mulas M AUID- ORCID: 0009-0008-3827-4150 AD - Department of Medical Science and Public Health, University of Cagliari, Cagliari, Italy. FAU - Puligheddu, Monica AU - Puligheddu M AUID- ORCID: 0000-0002-6837-6608 AD - Neurology Unit, University Hospital of Cagliari, Cagliari, Italy. AD - Department of Medical Science and Public Health, University of Cagliari, Cagliari, Italy. FAU - Mascia, Marcello Mario AU - Mascia MM AUID- ORCID: 0000-0002-9028-5972 AD - Neurology Unit, University Hospital of Cagliari, Cagliari, Italy. LA - eng PT - Case Reports PT - Journal Article DEP - 20261004 PL - Korea (South) TA - Clin Psychopharmacol Neurosci JT - Clinical psychopharmacology and neuroscience : the official scientific journal of the Korean College of Neuropsychopharmacology JID - 101207332 OTO - NOTNLM OT - Delusional parasitosis OT - Hallucinations OT - Infusions, subcutaneous OT - Levodopa OT - Parkinson disease OT - Psychotic disorders EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 03:42 PHST- 2026/05/11 00:00 [received] PHST- 2026/07/05 00:00 [revised] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 03:42 [entrez] AID - cpn.26.1449 [pii] AID - 10.9758/cpn.26.1449 [doi] PST - ppublish SO - Clin Psychopharmacol Neurosci. 2026 Nov 30;24(4):885-889. doi: 10.9758/cpn.26.1449. Epub 2026 Oct 4. PMID- 42947600 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261028 IS - 1738-1088 (Print) IS - 1738-1088 (Linking) VI - 24 IP - 4 DP - 2026 Nov 30 TI - Drug-drug Interaction Signals for Sexual Dysfunction with Antidepressants and Antipsychotics: A Pharmacovigilance-pharmacodynamic Study. PG - 854-863 LID - 10.9758/cpn.26.1443 [doi] AB - OBJECTIVE: Sexual dysfunction is a common adverse event associated with antidepressants and antipsychotics, yet its risk during combination therapy remains unclear. This study evaluated sexual dysfunction signals associated with antidepressant- antipsychotic combinations using the FDA Adverse Event Reporting System (FAERS) and explored potential pharmacodynamic mechanisms. METHODS: A pharmacovigilance-pharmacodynamic analysis of the FAERS database covering the fourth quarter of 1997 through the first quarter of 2022 was conducted. Combinations of antidepressants (selective serotonin reuptake inhibitors [SSRIs] and serotonin-norepinephrine reuptake inhibitors) with seven antipsychotics (aripiprazole, brexpiprazole, cariprazine, olanzapine, quetiapine, risperidone, and ziprasidone) were evaluated. Drug-drug interaction signals were assessed using five frequency statistical models (Omega shrinkage, additive, multiplicative, combination risk ratio, and chi-square). The association between antipsychotic receptor occupancy and sexual dysfunction was analyzed using a linear regression model. RESULTS: In total, 15,808,026 reports were analyzed. Drug-drug interaction signals for desire-related sexual dysfunction were detected for the SSRI-brexpiprazole combination in four algorithms (Omega shrinkage, additive, multiplicative, and chi-square). Linear regression analysis showed that the desire-related sexual dysfunction signal values were positively associated with 5-HT(1A) receptor occupancy (beta = 0.082, p = 0.008, R(2) = 0.782). CONCLUSION: Brexpiprazole augmentation of SSRIs may be associated with desire-related sexual dysfunction. The findings also suggest a potential association between 5-HT(1A) receptor occupancy and desire-related sexual dysfunction. As this study used a hypothesis-generating design based on spontaneous reporting data, further epidemiological studies are warranted. FAU - Horii, Rina AU - Horii R AUID- ORCID: 0000-0002-4149-7928 AD - Department of Pharmacotherapeutics and Informatics, School of Medicine, Fujita Health University, Toyoake, Japan. FAU - Hatano, Masakazu AU - Hatano M AUID- ORCID: 0000-0001-7032-878X AD - Department of Pharmacotherapeutics and Informatics, School of Medicine, Fujita Health University, Toyoake, Japan. FAU - Yamada, Shigeki AU - Yamada S AUID- ORCID: 0000-0002-3891-1323 AD - Department of Pharmacotherapeutics and Informatics, School of Medicine, Fujita Health University, Toyoake, Japan. LA - eng PT - Journal Article DEP - 20260723 PL - Korea (South) TA - Clin Psychopharmacol Neurosci JT - Clinical psychopharmacology and neuroscience : the official scientific journal of the Korean College of Neuropsychopharmacology JID - 101207332 OTO - NOTNLM OT - Antidepressants OT - Antipsychotics OT - Drug-drug interactions OT - Pharmacovigilance OT - Sexual dysfunction EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 03:42 PHST- 2026/04/22 00:00 [received] PHST- 2026/05/27 00:00 [revised] PHST- 2026/05/27 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 03:42 [entrez] AID - cpn.26.1443 [pii] AID - 10.9758/cpn.26.1443 [doi] PST - ppublish SO - Clin Psychopharmacol Neurosci. 2026 Nov 30;24(4):854-863. doi: 10.9758/cpn.26.1443. Epub 2026 Jul 23. PMID- 42947598 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261028 IS - 1738-1088 (Print) IS - 1738-1088 (Linking) VI - 24 IP - 4 DP - 2026 Nov 30 TI - Aripiprazole versus Bupropion Augmentation in Patients with Anxious Depression Resistant to Selective Serotonin Reuptake Inhibitors: A Randomized, Prospective, Open-label, Multicenter Study. PG - 832-843 LID - 10.9758/cpn.26.1422 [doi] AB - OBJECTIVE: Research on which adjunctive therapies are more effective for treatment resistant anxious depression is lacking. This study aimed to evaluate and compare the efficacy and safety of bupropion versus aripiprazole as adjunctive therapies in patients with anxious depression who are resistant to selective serotonin reuptake inhibitors (SSRIs). METHODS: A total of 48 patients with anxious depression, defined as a score >/= 7 on the anxiety/somatization factor of the Hamilton Depression Rating Scale (HAM-D), were enrolled in this 6-week, randomized, prospective, open-label, multicenter trial. Participants, having shown at least moderate to severe depressive symptoms despite four weeks or more of SSRI monotherapy at maximum recommended or tolerated doses, were randomized to receive either aripiprazole or bupropion. RESULTS: Both treatment groups exhibited significant improvements in HAM-D and Montgomery-Asberg Depression Rating Scale scores from baseline to week 6 (p < 0.001). There were no significant differences between the groups in the changes observed for Montgomery-Asberg Depression Rating Scale scores (p = 0.370), HAM-D (p > 0.999), anxiety/somatization factor scores (p = 0.177), Inventory of Fatigue Scale (p = 0.600), Clinical Global Impression-Severity (p = 0.620), or the rates of remission (p = 0.130) and response (p = 0.452). Additionally, the Drug-Induced Extrapyramidal Symptoms Scale and Psychotropic-Related Sexual Dysfunction Questionnaire scores showed no significant differences. CONCLUSION: Aripiprazole and bupropion are both effective and well-tolerated as augmentation strategies in SSRI-resistant anxious depression. While these findings provide clinical insights into individualized augmentation for anxious depression, they should be considered exploratory, necessitating larger-scale, definitive trials. FAU - Jo, So-Hye AU - Jo SH AUID- ORCID: 0000-0001-9471-0713 AD - Department of Psychiatry, College of Medicine, Yeungnam University, Daegu, Korea. FAU - Koo, Bon-Hoon AU - Koo BH AUID- ORCID: 0000-0001-9633-3835 AD - Department of Psychiatry, College of Medicine, Yeungnam University, Daegu, Korea. FAU - Lee, Kwang-Hun AU - Lee KH AUID- ORCID: 0000-0003-4531-8015 AD - Lee Gwang-hun Psychiatry Clinic, Gyeongju, Korea. FAU - Lee, Jong-Hun AU - Lee JH AUID- ORCID: 0000-0002-3581-5029 AD - Department of Psychiatry, College of Medicine, Daegu Catholic University, Daegu, Korea. FAU - Lee, Seung-Jae AU - Lee SJ AUID- ORCID: 0000-0003-3648-9824 AD - Department of Psychiatry, Kyungpook National University School of Medicine, Daegu, Korea. FAU - Park, Young-Woo AU - Park YW AUID- ORCID: 0000-0002-1987-7035 AD - Park Young-woo Psychiatric Clinic, Daegu, Korea. FAU - Sung, Hyung-Mo AU - Sung HM AUID- ORCID: 0000-0002-2396-3358 AD - Department of Psychiatry, Soonchunhyang University Gumi Hospital, Gumi, Korea. FAU - Cheon, Eun-Jin AU - Cheon EJ AUID- ORCID: 0000-0002-6986-0768 AD - Department of Psychiatry, College of Medicine, Yeungnam University, Daegu, Korea. LA - eng PT - Journal Article DEP - 20260708 PL - Korea (South) TA - Clin Psychopharmacol Neurosci JT - Clinical psychopharmacology and neuroscience : the official scientific journal of the Korean College of Neuropsychopharmacology JID - 101207332 OTO - NOTNLM OT - Anxiety OT - Aripiprazole OT - Augmentation therapy OT - Bupropion OT - Major depressive disorder EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 03:42 PHST- 2026/02/26 00:00 [received] PHST- 2026/04/07 00:00 [revised] PHST- 2026/05/26 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 03:42 [entrez] AID - cpn.26.1422 [pii] AID - 10.9758/cpn.26.1422 [doi] PST - ppublish SO - Clin Psychopharmacol Neurosci. 2026 Nov 30;24(4):832-843. doi: 10.9758/cpn.26.1422. Epub 2026 Jul 8. PMID- 42947589 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261028 IS - 1738-1088 (Print) IS - 1738-1088 (Linking) VI - 24 IP - 4 DP - 2026 Nov 30 TI - Predicting Polysomnography‑derived Sleep Efficiency and Total Sleep Time Using Clinical Questionnaires: A Machine Learning Approach. PG - 732-745 LID - 10.9758/cpn.25.1354 [doi] AB - OBJECTIVE: We developed and evaluated machine learning models to predict polysomnography (PSG)-derived sleep efficiency and total sleep time using clinical questionnaire-based assessments, with the aim of developing accessible non-invasive screening tools that can estimate objective sleep parameters in the absence of PSG. METHODS: Predictive models were developed using demographic data and nine validated questionnaires from 1,135 participants. Three gradient boosting algorithms-Categorical Boosting (CatBoost), Light Gradient Boosting Machine (LGBM), and eXtreme Gradient Boosting (XGBoost)-were compared for binary classification of sleep efficiency (>/= 85% vs. < 85%) and four-class prediction of total sleep time (< 5, 5-7, 7-9, and >/= 9 hours). Model performance was evaluated using area under the receiver operating characteristic curve (AUC) and precision-recall curve (PRC) metrics. RESULTS: CatBoost consistently outperformed LGBM and XGBoost. For sleep efficiency classification classification, CatBoost achieved the highest performance (AUC = 0.7342; PRC = 0.6957). In total sleep time prediction, CatBoost demonstrated superior accuracy (weighted AUC = 0.6772; weighted PRC = 0.5384; micro-AUC = 0.8195). Inclusion of formal sleep disorder diagnoses provided negligible improvements. CONCLUSION: Validated self-report instruments effectively captured clinically meaningful determinants of sleep efficiency and total sleep time. Machine learning models, particularly CatBoost, demonstrated robust performance using questionnaire-derived features alone, highlighting the central roles of age, insomnia severity, and daytime sleepiness. These findings support the feasibility and clinical utility of accessible, questionnaire-driven machine learning approaches for large-scale screening, early risk stratification, and decision support in sleep medicine. FAU - Kim, Ju-Wan AU - Kim JW AUID- ORCID: 0000-0002-9888-1090 AD - Department of Psychiatry, Chonnam National University Medical School, Gwangju, Korea. FAU - Kim, Yeongmin AU - Kim Y AUID- ORCID: 0009-0001-3234-0101 AD - Department of Biomedical Informatics, Korea University College of Medicine, Seoul, Korea. FAU - Jeong, Inyong AU - Jeong I AUID- ORCID: 0009-0009-1504-0463 AD - Department of Biomedical Informatics, Korea University College of Medicine, Seoul, Korea. FAU - Jung, Taewon AU - Jung T AUID- ORCID: 0009-0004-0621-3557 AD - Department of Biomedical Informatics, Korea University College of Medicine, Seoul, Korea. FAU - Hong, Geesoo AU - Hong G AUID- ORCID: 0009-0006-0697-2953 AD - Department of Biomedical Informatics, Korea University College of Medicine, Seoul, Korea. FAU - Kim, Dae-Kwang AU - Kim DK AUID- ORCID: 0009-0004-3455-1828 AD - Department of Psychiatry, Chonnam National University Medical School, Gwangju, Korea. FAU - Kang, Hee-Ju AU - Kang HJ AUID- ORCID: 0000-0002-4784-4820 AD - Department of Psychiatry, Chonnam National University Medical School, Gwangju, Korea. FAU - Ryu, Seunghyong AU - Ryu S AUID- ORCID: 0000-0001-6127-760X AD - Department of Psychiatry, Chonnam National University Medical School, Gwangju, Korea. FAU - Shin, Il-Seon AU - Shin IS AUID- ORCID: 0000-0001-5370-7649 AD - Department of Psychiatry, Chonnam National University Medical School, Gwangju, Korea. FAU - Kim, Sung-Wan AU - Kim SW AUID- ORCID: 0000-0002-6739-2163 AD - Department of Psychiatry, Chonnam National University Medical School, Gwangju, Korea. FAU - Jhon, Min AU - Jhon M AUID- ORCID: 0000-0002-0408-768X AD - Department of Psychiatry, Chonnam National University Medical School, Gwangju, Korea. FAU - Lee, Hwamin AU - Lee H AUID- ORCID: 0000-0002-6482-3511 AD - Department of Biomedical Informatics, Korea University College of Medicine, Seoul, Korea. FAU - Kim, Jae-Min AU - Kim JM AUID- ORCID: 0000-0001-7409-6306 AD - Department of Psychiatry, Chonnam National University Medical School, Gwangju, Korea. LA - eng PT - Journal Article DEP - 20260119 PL - Korea (South) TA - Clin Psychopharmacol Neurosci JT - Clinical psychopharmacology and neuroscience : the official scientific journal of the Korean College of Neuropsychopharmacology JID - 101207332 OTO - NOTNLM OT - Diagnosis, computer-assisted OT - Machine learning OT - Polysomnography OT - Sleep OT - Surveys and questionnaires EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 03:42 PHST- 2025/09/07 00:00 [received] PHST- 2025/11/18 00:00 [revised] PHST- 2025/12/18 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 03:42 [entrez] AID - cpn.25.1354 [pii] AID - 10.9758/cpn.25.1354 [doi] PST - ppublish SO - Clin Psychopharmacol Neurosci. 2026 Nov 30;24(4):732-745. doi: 10.9758/cpn.25.1354. Epub 2026 Jan 19. PMID- 42947584 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261028 IS - 1738-1088 (Print) IS - 1738-1088 (Linking) VI - 24 IP - 4 DP - 2026 Nov 30 TI - Artificial Intelligence-driven Biomarker Discovery for Precision Psychiatry. PG - 675-691 LID - 10.9758/cpn.26.1519 [doi] AB - Poor phenotype quality and small cohorts remain major constraints in psychiatric biomarker research, and recent methodological advances increasingly address them. Language models turn clinical text into analysable variables at a scale manual chart review cannot reach. Self-supervised models learn from the unlabelled signals psychiatry already collects. Variant-effect prediction makes molecular interpretation computable, and research agents have generated and experimentally confirmed candidates outside psychiatry. These capabilities act early, on what is measured, what can be learned from it and which candidates are proposed. None of the artificial intelligence capabilities reviewed here yet reaches the stages at which a candidate becomes a clinical test. Producing candidates is becoming cheap while qualifying them is not. This review is organised around that asymmetry rather than by disorder or marker catalogue. It separates the measured characteristic, the prediction model and the intended clinical use, and treats method family as distinct from evidential stage. Across the selected evidence, discrimination is supported more strongly in development data than in independent evaluation, and prospective evidence that model-guided use improves care is scarce. Many reported metrics also address a simpler task than the intended decision: diagnosis instead of differential diagnosis, general prognosis instead of differential treatment benefit. The review proposes a six-stage framework linking intended use, measurement validity, model evaluation, comparative utility and monitored deployment, and places each capability on it, clarifying where methodological progress can accelerate discovery and where conventional clinical validation remains indispensable. FAU - Serretti, Alessandro AU - Serretti A AUID- ORCID: 0000-0003-4363-3759 AD - Department of Medicine and Surgery, Kore University of Enna, Enna, Italy. AD - Oasi Research Institute-IRCCS, Troina, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261030 PL - Korea (South) TA - Clin Psychopharmacol Neurosci JT - Clinical psychopharmacology and neuroscience : the official scientific journal of the Korean College of Neuropsychopharmacology JID - 101207332 OTO - NOTNLM OT - Artificial intelligence OT - Biomarkers OT - External validation OT - Large language models OT - Machine learning OT - Precision psychiatry EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 03:42 PHST- 2026/09/03 00:00 [received] PHST- 2026/09/09 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 03:42 [entrez] AID - cpn.26.1519 [pii] AID - 10.9758/cpn.26.1519 [doi] PST - ppublish SO - Clin Psychopharmacol Neurosci. 2026 Nov 30;24(4):675-691. doi: 10.9758/cpn.26.1519. Epub 2026 Oct 30. PMID- 42947583 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261028 IS - 1738-1088 (Print) IS - 1738-1088 (Linking) VI - 24 IP - 4 DP - 2026 Nov 30 TI - Pharmacologic Treatment Response in Panic Disorder with Comorbid Medical Conditions: A Systematic and Narrative Review. PG - 665-674 LID - 10.9758/cpn.26.1413 [doi] AB - Panic disorder frequently co-occurs with medical conditions, complicating diagnosis and treatment. Although pharmacotherapy is a first-line treatment for panic disorder, pharmacotherapeutic evidence for patients with medical comorbidities is limited. Therefore, this review systematically evaluates the efficacy of pharmacologic treatments for panic disorder in adults with medical comorbidities and synthesizes clinical insights using a narrative approach. We searched PubMed, Embase, and the Cochrane Library through April 22, 2024, following Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Eligible studies compared pharmacotherapy with placebo or no treatment in patients with panic disorder and comorbidities. The review protocol was registered in the International Prospective Register of Systematic Reviews (registration number CRD42024544914). A hybrid approach combining systematic review methods with narrative synthesis was employed to enable broader interpretation. Two randomized controlled trials met the inclusion criteria. Both had small sample sizes and used different outcome measures, limiting cross-study comparability. Selective serotonin reuptake inhibitors and benzodiazepines showed potential in alleviating panic symptoms among patients with irritable bowel syndrome or noncardiac chest pain. Narrative synthesis underscored the bidirectional link between panic disorder and medical conditions, challenges in pharmacologic treatment, and gaps in existing clinical guidelines. Current evidence on pharmacologic treatment of panic disorder with medical comorbidities is very limited, derived from only two small studies with heterogeneous designs. Selective serotonin reuptake inhibitors and benzodiazepines showed preliminary signals of potential benefit, but definitive conclusions cannot be drawn. Individualized treatment and interdisciplinary collaboration are essential. Large-scale clinical trials and updated guidelines are needed to better support this population. FAU - Kim, Sun Mi AU - Kim SM AUID- ORCID: 0000-0003-4131-0542 AD - Department of Psychiatry, Chung-Ang University College of Medicine, Seoul, Korea. FAU - Kim, Hye Ri AU - Kim HR AUID- ORCID: 0000-0002-4147-4056 AD - Department of Psychiatry, Chung-Ang University College of Medicine, Seoul, Korea. FAU - Jeon, Hong Jun AU - Jeon HJ AUID- ORCID: 0000-0002-0260-0494 AD - Department of Psychiatry, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul, Korea. FAU - Hong, Sumin AU - Hong S AUID- ORCID: 0000-0002-6109-6902 AD - Department of Psychiatry, Konkuk University Medical Center, Seoul, Korea. LA - eng PT - Journal Article PT - Review DEP - 20260731 PL - Korea (South) TA - Clin Psychopharmacol Neurosci JT - Clinical psychopharmacology and neuroscience : the official scientific journal of the Korean College of Neuropsychopharmacology JID - 101207332 OTO - NOTNLM OT - Collaborative care OT - Integrated care OT - Medical comorbidity OT - Panic disorder OT - Pharmacotherapy OT - Systematic review EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 03:42 PHST- 2026/02/05 00:00 [received] PHST- 2026/05/20 00:00 [revised] PHST- 2026/05/24 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 03:42 [entrez] AID - cpn.26.1413 [pii] AID - 10.9758/cpn.26.1413 [doi] PST - ppublish SO - Clin Psychopharmacol Neurosci. 2026 Nov 30;24(4):665-674. doi: 10.9758/cpn.26.1413. Epub 2026 Jul 31. PMID- 42947576 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2639-8028 (Electronic) IS - 2639-8028 (Linking) VI - 8 IP - 11 DP - 2026 Nov 1 TI - From Framework to Toolkit: Practical Guidance for Using the PrOcess Evaluation of Trials In Critical care (POETIC) Framework. PG - e1503 LID - 10.1097/CCE.0000000000001503 [doi] LID - e1503 AB - BACKGROUND: The PrOcess Evaluation of Trials In Critical care (POETIC) framework was developed to support systematic evaluation of Context, Fidelity, Dose, Reach, and an integrative assessment of Quality of Delivery (QoD). Its practical use, however, requires explicit guidance on selecting measures, constructing QoD, assessing changing context, and governing prospective use. METHODS: We undertook a retrospective comparative synthesis of process evaluations embedded in four randomized U.K. critical care trials: Biomarker-guided antibiotic stewardship in suspected ventilator-associated pneumonia (VAPrapid-2), Provision of Psychological Support to People in Intensive Care (POPPI), Sedation AND Weaning In CHildren (SANDWICH), and Alpha-2 agonists for sedation to produce Better outcomes from critical illness (A2B). We selected them because they informed POETIC's development or application and provided sufficiently detailed quantitative and qualitative evidence. We mapped the reports, protocols, and process evaluation findings to the five dimensions; compared recurring and contradictory patterns, quantitative-qualitative agreement, measurement and interpretation problems, and mechanism-specific limitations; and translated our learning into guidance for applying the framework. RESULTS: The relevance and measurement of POETIC dimensions varied according to how each intervention was expected to work. Contextual influences changed over time and differed between sites. Combined assessments of QoD required explicit decisions about component selection, weighting, thresholds, aggregation, and missing data. VAPrapid-2 showed why a separate Dose measure and a QoD composite were not meaningful for a one-off diagnostic recommendation; POPPI used a weighted site composite; SANDWICH summarized delivery of four intervention components, staff training, and patient Reach; and A2B combined four equally weighted indicators in a 0-12 site score. This cross-trial learning informed practical guidance on selecting measures, assessing context over time, integrating quantitative and qualitative findings, constructing QoD, and governing prospective use. CONCLUSIONS: POETIC is best used as a mechanism-aligned, mixed-method scaffold rather than a requirement to measure every dimension or calculate a universal composite. Prospective use is feasible when outputs, recipients, review times, permitted actions, and safeguards are prespecified. The framework and accompanying toolkit support reproducible assessment while protecting trial integrity. CI - Copyright (c) 2026 The Authors. Published by Wolters Kluwer Health, LLC. on behalf of the Society of Critical Care Medicine. FAU - Emerson, Lydia M AU - Emerson LM AUID- ORCID: 0000-0002-4250-5758 AD - Department of Health and Community Sciences, The Medical School, University of Exeter, Exeter, United Kingdom. AD - Wellcome Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, United Kingdom. FAU - McAuley, Daniel F AU - McAuley DF AD - Wellcome Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, United Kingdom. FAU - Clarke, Mike AU - Clarke M AD - Centre for Public Health, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, United Kingdom. FAU - Blackwood, Bronagh AU - Blackwood B AD - Wellcome Wolfson Institute for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, United Kingdom. FAU - Colquhoun, Gosha AU - Colquhoun G AD - School of Health and Social Care, Edinburgh Napier University, Edinburgh, United Kingdom. AD - Division of Research Methodology, Wroclaw Medical University, Wroclaw, Poland. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Crit Care Explor JT - Critical care explorations JID - 101746347 SB - IM MH - Humans MH - *Critical Care/standards MH - Retrospective Studies MH - *Process Assessment, Health Care/methods MH - Pneumonia, Ventilator-Associated/drug therapy MH - United Kingdom MH - Randomized Controlled Trials as Topic MH - Antimicrobial Stewardship PMC - PMC13716417 OTO - NOTNLM OT - complex intervention OT - critical care OT - health services research OT - process evaluation OT - randomized controlled trial COIS- The authors have disclosed that they do not have any potential conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/27 CRDT- 2026/10/28 03:33 PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 03:33 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 02107256-202611000-00003 [pii] AID - CCE-D-26-00067 [pii] AID - 10.1097/CCE.0000000000001503 [doi] PST - epublish SO - Crit Care Explor. 2026 Oct 27;8(11):e1503. doi: 10.1097/CCE.0000000000001503. eCollection 2026 Nov 1. PMID- 42947555 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1099-0801 (Electronic) IS - 0269-3879 (Linking) VI - 40 IP - 12 DP - 2026 Dec TI - Conjugatability and Physicochemical Stability of an Infliximab-vcMMAE Antibody-Drug Conjugate. LID - e70648 [pii] LID - 10.1002/bmc.70648 [doi] AB - Transmembrane tumor necrosis factor (tmTNF) can support internalization of anti-TNF antibodies, providing a mechanistic rationale for exploring anti-TNF antibody-drug conjugate formats. We evaluated infliximab as a scaffold for conventional interchain-cysteine conjugation using an MC-valine-citrulline-PABC-MMAE linker-payload (vcMMAE). Increasing tris(2-carboxyethyl)phosphine (TCEP) from 2.2 to 2.6 equivalents increased the average drug-to-antibody ratio (DAR) from 3.12 to 3.65 by reduced RP-HPLC and from 3.5 to 4.1 by hydrophobic interaction chromatography (HIC). SEC-detectable high-molecular-weight (HMW) species remained approximately 1% across the conjugates, comparable to unconjugated infliximab (1.1%). A selected preparation with an average DAR of approximately 4 showed no substantial change in its SEC profile after five freeze-thaw cycles or incubation at 37 degrees C for 2 weeks. These results demonstrate that infliximab is amenable to conventional interchain-cysteine conjugation to approximately DAR = 4 without an evident increase in SEC-detectable HMW species and provide an initial chromatographic assessment of its post-conjugation physicochemical stability. CI - (c) 2026 John Wiley & Sons Ltd. FAU - Matsuda, Yutaka AU - Matsuda Y AUID- ORCID: 0000-0003-2801-4706 AD - Ajinomoto Bio-Pharma Services, San Diego, California, USA. AD - Ajinomoto Co. Inc, Kawasaki, Kanagawa, Japan. FAU - Leung, Monica AU - Leung M AD - Ajinomoto Bio-Pharma Services, San Diego, California, USA. FAU - Tawfiq, Zhala AU - Tawfiq Z AD - Ajinomoto Bio-Pharma Services, San Diego, California, USA. FAU - Robles, Veronica AU - Robles V AD - Ajinomoto Bio-Pharma Services, San Diego, California, USA. FAU - Nakahara, Yuichi AU - Nakahara Y AD - Ajinomoto Co. Inc, Kawasaki, Kanagawa, Japan. FAU - Mendelsohn, Brian A AU - Mendelsohn BA AUID- ORCID: 0000-0002-6339-2377 AD - Ajinomoto Bio-Pharma Services, San Diego, California, USA. LA - eng PT - Journal Article PL - England TA - Biomed Chromatogr JT - Biomedical chromatography : BMC JID - 8610241 RN - B72HH48FLU (Infliximab) RN - 0 (Immunoconjugates) RN - K848JZ4886 (Cysteine) SB - IM MH - *Infliximab/chemistry MH - *Immunoconjugates/chemistry MH - Drug Stability MH - Chromatography, High Pressure Liquid MH - Hydrophobic and Hydrophilic Interactions MH - Cysteine/chemistry MH - Chromatography, Gel OTO - NOTNLM OT - antibody-drug conjugate OT - cysteine conjugation OT - drug-to-antibody ratio OT - infliximab OT - physicochemical stability EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 03:22 PHST- 2026/10/05 00:00 [revised] PHST- 2026/08/15 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 03:22 [entrez] AID - e70648 [pii] AID - 10.1002/bmc.70648 [doi] PST - ppublish SO - Biomed Chromatogr. 2026 Dec;40(12):e70648. doi: 10.1002/bmc.70648. PMID- 42947553 OWN - NLM STAT- Publisher LR - 20261028 IS - 2384-1621 (Print) DP - 2026 Oct 29 TI - Observed Service Utilization Differences and Audiometric Profiles Among Adults With Hearing Loss in Eastern Coastal Provinces in China. LID - 10.7874/jao.2026.00185 [doi] AB - BACKGROUND AND OBJECTIVES: : Hearing loss is a major public health concern that hampers communication, social participation, and quality of life. To date, large-scale demographic and audiometric characteristic data for adults seeking hearing care services in China are limited. We aimed to assemble demographic and audiometric profiles for adults requesting hearing care services for the first time in selected eastern coastal provinces of China, with a focus on service- and region-specific patterns in observed service utilization. SUBJECTS AND METHODS: : This multicenter retrospective study included 2,413 adult first-time patients who initially sought hearing care services between January and June 2025, including 2,242 patients who visited public hospitals and 171 patients who visited private hearing aid clinics. Demographic and audiometric data were extracted from institutional databases. Hearing loss severity was assessed using better-ear four-frequency average hearing loss scores. Differences between groups were analyzed using analysis of variance and chi-square tests. Multiple linear regression was performed to identify factors associated with hearing loss severity. RESULTS: : Mean patient age was 55.65 years (51.1% were male). Patients attending private hearing aid clinics were significantly older than those visiting public hospitals and had more severe hearing loss. Sensorineural hearing loss was the most common (56.8%), followed by normal hearing (29.1%), mixed hearing loss (12.4%), and conductive hearing loss (1.7%). Regional differences in sex distribution and hearing loss profiles were observed. CONCLUSIONS: : This multicenter retrospective study describes observed service utilization and service-specific audiometric profile differences among adults presenting at selected hearing care institutions in Chinese eastern coastal provinces. Patients attending participating private hearing aid clinics operated by one provider were generally older and had more severe hearing loss, whereas public hospitals received a broader and more heterogeneous patient population. These findings should be interpreted descriptively and may help encourage earlier hearing screening, referral coordination, and integration of diagnostic and rehabilitation-oriented hearing care services. FAU - Chen, Min AU - Chen M AD - Department of Otorhinolaryngology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. chenm0116@163.com. FAU - Quan, Haoyu AU - Quan H AD - Department of Otorhinolaryngology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. FAU - Li, Fang AU - Li F AD - Department of Otorhinolaryngology, Affiliated Hospital of Xuzhou Medical University, Xuzhou, China. LA - eng PT - Journal Article DEP - 20261029 PL - Korea (South) TA - J Audiol Otol JT - Journal of audiology & otology JID - 101657815 OTO - NOTNLM OT - Audiometry OT - China OT - Facilities and services utilization OT - Hearing aids OT - Hearing loss EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 03:19 PHST- 2026/04/01 00:00 [received] PHST- 2026/06/26 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 03:19 [entrez] AID - jao.2026.00185 [pii] AID - 10.7874/jao.2026.00185 [doi] PST - aheadofprint SO - J Audiol Otol. 2026 Oct 29. doi: 10.7874/jao.2026.00185. PMID- 42947529 OWN - NLM STAT- Publisher LR - 20261028 IS - 1097-0347 (Electronic) IS - 1043-3074 (Linking) DP - 2026 Oct 27 TI - Comparative Effectiveness and Safety of Energy Devices for Thyroidectomy: An Outcome-Specific Network Meta-Analysis of Randomized Controlled Trials. LID - 10.1002/hed.70524 [doi] AB - BACKGROUND: We performed an outcome-specific network meta-analysis comparing contemporary energy devices in thyroidectomy. METHODS: Randomized controlled trials comparing conventional hemostasis with Harmonic, LigaSure, Thunderbeat, or other advanced energy devices during thyroidectomy were included. Frequentist random-effects network meta-analyses were performed per outcome, with treatments ranked by P-scores and consistency assessed via design-by-treatment interaction and node-splitting analyses. RESULTS: Compared to conventional hemostasis, Harmonic significantly reduced operative time (SMD, -1.88), blood loss (SMD, -2.29), drainage volume (SMD, -1.77), hospital stay (SMD, -0.74), transient hypocalcemia (OR, 0.61), transient recurrent laryngeal nerve palsy (OR, 0.63), and postoperative bleeding (OR, 0.56). LigaSure improved operative efficiency but showed less consistent recovery and safety benefits. Harmonic ranked highest across most outcomes. CONCLUSIONS: Harmonic showed the most favorable relative effects across the evaluated outcomes. However, these findings do not establish overall device superiority; selection should consider absolute benefits and device-specific characteristics. CI - (c) 2026 Wiley Periodicals LLC. FAU - Kim, Do Hyun AU - Kim DH AUID- ORCID: 0000-0002-9248-5572 AD - Department of Otolaryngology-Head and Neck Surgery, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, South Korea. FAU - Jang, David W AU - Jang DW AUID- ORCID: 0000-0003-4200-7174 AD - Department of Head and Neck Surgery & Communication Sciences, Duke University School of Medicine, Durham, North Carolina, USA. FAU - Hwang, Se Hwan AU - Hwang SH AUID- ORCID: 0000-0002-2838-7820 AD - Department of Otolaryngology-Head and Neck Surgery, Bucheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, South Korea. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Head Neck JT - Head & neck JID - 8902541 SB - IM OTO - NOTNLM OT - meta-analysis as topic OT - randomized controlled trials as topic OT - surgical hemostasis OT - thyroidectomy OT - ultrasonic surgical procedures EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 03:03 PHST- 2026/10/06 00:00 [revised] PHST- 2026/07/21 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 03:03 [entrez] AID - 10.1002/hed.70524 [doi] PST - aheadofprint SO - Head Neck. 2026 Oct 27. doi: 10.1002/hed.70524. PMID- 42947524 OWN - NLM STAT- Publisher LR - 20261028 IS - 1365-263X (Electronic) IS - 0960-7439 (Linking) DP - 2026 Oct 27 TI - Direct Pulp Capping Versus Pulpotomy Using a Calcium Silicate-Based Putty for Carious Pulp Exposures in Primary Molars: A Split Mouth Randomized Clinical Trial. LID - 10.1111/ipd.70156 [doi] AB - BACKGROUND: Direct pulp capping with calcium silicate-based materials has gained attention as a minimally invasive treatment for carious pulp exposures in primary molars. AIM: To compare the outcome of direct pulp capping and pulpotomy in primary molars with carious pulp exposure. DESIGN: A split-mouth randomized clinical trial, 100 carious primary molars in children aged 4-9 years were included. After pulp exposure, teeth were randomly assigned to direct pulp capping or pulpotomy; a calcium silicate-based putty (NeoPUTTY) was applied, and teeth were restored with glass ionomer cement and stainless-steel crowns. Preoperative pain and baseline variables were recorded. Clinical and radiographic evaluations were conducted at 3, 6, and 12 months. RESULTS: At 3 months, 96 teeth evaluated, with one failure in the pulpotomy group. At 6 months (96% recall), nine teeth failed (5 pulpotomy, 4 direct pulp capping), with success rates of 89.6% and 91.7%, respectively (p = 0.73). At 12 months (94% recall), seven failures occurred in each group, yielding identical success rates of 85.1% and survival rates of 93.6%. Preoperative factors were not associated with treatment outcomes. CONCLUSION: Direct pulp capping demonstrated comparable short-term outcomes to pulpotomy in cariously exposed primary molars. CI - (c) 2026 BSPD, IAPD and John Wiley & Sons Ltd. FAU - Taha, Nessrin AU - Taha N AUID- ORCID: 0000-0003-3233-8850 AD - Department of Conservative Dentistry, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan. FAU - Kharabsheh, Dana AU - Kharabsheh D AD - Department of Preventive Dentistry, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan. FAU - Al-Batayneh, Ola B AU - Al-Batayneh OB AUID- ORCID: 0000-0003-1670-497X AD - Department of Preventive Dentistry, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan. LA - eng GR - 583/2023/Deanship of Research, Jordan University of Science and Technology/ PT - Journal Article DEP - 20261027 PL - England TA - Int J Paediatr Dent JT - International journal of paediatric dentistry JID - 9107511 SB - IM OTO - NOTNLM OT - calcium silicate cement OT - carious pulp exposure OT - primary molar OT - pulp capping OT - pulpotomy EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 03:03 PHST- 2026/09/06 00:00 [revised] PHST- 2026/03/10 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 03:03 [entrez] AID - 10.1111/ipd.70156 [doi] PST - aheadofprint SO - Int J Paediatr Dent. 2026 Oct 27. doi: 10.1111/ipd.70156. PMID- 42947523 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1521-4141 (Electronic) IS - 0014-2980 (Print) IS - 0014-2980 (Linking) VI - 56 IP - 10 DP - 2026 Oct TI - Prophylactic Soraphen A Treatment Attenuates Western Diet-Exacerbated IMQ-Induced Psoriasiform Inflammation. LID - e70298 [pii] LID - 10.1002/eji.70298 [doi] AB - Psoriasis is a T-cell-mediated inflammatory disease amplified by metabolic stress. Acetyl-CoA carboxylase 1 (ACC1)-dependent fatty acid synthesis is required for IL-17-producing T cells. Here, we asked whether short-term systemic administration of soraphen A (SorA), a dual ACC1/2 inhibitor, modulates imiquimod (IMQ)-induced psoriasiform inflammation under normal diet (ND) and Western diet (WD) conditions. Female C57BL/6J mice were fed ND or WD for 12 weeks prior to daily intraperitoneal injections of SorA or vehicle for 10 days, followed by 5 days of topical IMQ to induce psoriasiform dermatitis. Disease severity was assessed by macroscopic, histological, transcriptional, and flow cytometric readouts. Prophylactic SorA administration had no measurable effect in ND-fed mice. In WD-fed mice, prophylactic SorA reduced erythema, back skin thickening, and epidermal hyperplasia, whereas scaling remained unchanged. Selected histological skin readouts were numerically lower, whereas the qPCR panel showed no statistically significant differences. In skin-draining lymph nodes, IL-17A(+) CD4(+) and CD8(+) T-cell frequencies and numbers were significantly reduced, whereas gammadeltaT17 cells did not differ significantly. Hepatic steatosis scores were unchanged, but hepatic neutrophil infiltration was reduced. Under the present prophylactic regimen, systemic SorA administration was associated with modest attenuation of WD-exacerbated IMQ-induced psoriasiform inflammation. These findings support further preclinical evaluation of ACC-targeted immunometabolic interventions in metabolically aggravated psoriasiform inflammation. CI - (c) 2026 The Author(s). European Journal of Immunology published by Wiley-VCH GmbH. FAU - Kao, Yu-San AU - Kao YS AD - Institute of Medical Microbiology and Hygiene, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. FAU - Kommoss, Katharina S AU - Kommoss KS AD - Division of Chronic Inflammation and Cancer, German Cancer Research Center (DKFZ), Heidelberg, Germany. AD - Department of Dermatology, University Hospital Heidelberg, Heidelberg, Germany. FAU - Bieler, Tabea AU - Bieler T AD - Division of Chronic Inflammation and Cancer, German Cancer Research Center (DKFZ), Heidelberg, Germany. AD - Fachbereich Biologie, Johannes Gutenberg University Mainz, Mainz, Germany. FAU - Godoy, Gloria Janet AU - Godoy GJ AD - Institute of Molecular Medicine, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. FAU - Lopez Krol, Aleksandra AU - Lopez Krol A AD - Institute of Medical Microbiology and Hygiene, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. FAU - Boukhallouk, Fatima AU - Boukhallouk F AD - Institute of Medical Microbiology and Hygiene, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. FAU - Kommoss, Felix K F AU - Kommoss FKF AD - Institute of Pathology, University Hospital Heidelberg, Heidelberg, Germany. FAU - Heide, Danijela AU - Heide D AD - Division of Chronic Inflammation and Cancer, German Cancer Research Center (DKFZ), Heidelberg, Germany. FAU - Kirsch, Susanne H AU - Kirsch SH AD - Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI), Saarland University Campus, Saarbrucken, Germany. AD - German Centre for Infection Research (DZIF), Partner Site Hannover-Braunschweig, Braunschweig, Germany. FAU - Muller, Rolf AU - Muller R AD - Helmholtz Institute for Pharmaceutical Research Saarland (HIPS), Helmholtz Centre for Infection Research (HZI), Saarland University Campus, Saarbrucken, Germany. AD - German Centre for Infection Research (DZIF), Partner Site Hannover-Braunschweig, Braunschweig, Germany. AD - Department of Pharmacy, Saarland University, Saarbrucken, Germany. FAU - Heikenwalder, Mathias AU - Heikenwalder M AD - Division of Chronic Inflammation and Cancer, German Cancer Research Center (DKFZ), Heidelberg, Germany. AD - The M3 Research Center, Institute For Interdisciplinary Research On Cancer Metabolism and Chronic Inflammation, Eberhard Karls University Tubingen, Medical Faculty Tubingen, Germany. FAU - Sparwasser, Tim AU - Sparwasser T AD - Institute of Medical Microbiology and Hygiene, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany. AD - Research Center for Immunotherapy (FZI), University Medical Center, Johannes Gutenberg-University Mainz, Mainz, Germany. LA - eng GR - Physician-Scientist Programme of Heidelberg University, Faculty of Medicine/ GR - 246807620,SFB156C09N/German Research Foundation/ GR - SFB156B08/German Research Foundation/ GR - SFB156C09N/German Research Foundation/ GR - TTU 09.721/German Centre for Infection Research/ GR - ExcellenceStrategyoftheFederalandStateGovernmentsofGermany/Federal Ministry of Education and Research (BMBF) and the Ministry of Science Baden-Wurttemberg/ PT - Journal Article PL - Germany TA - Eur J Immunol JT - European journal of immunology JID - 1273201 RN - P1QW714R7M (Imiquimod) RN - 122547-72-2 (soraphen A) RN - 0 (Interleukin-17) RN - EC 6.4.1.2 (Acetyl-CoA Carboxylase) RN - 0 (Macrolides) SB - IM MH - Animals MH - Female MH - *Diet, Western/adverse effects MH - Mice MH - *Psoriasis/chemically induced/drug therapy/immunology/pathology/prevention & control MH - Imiquimod MH - Mice, Inbred C57BL MH - *Inflammation/drug therapy/chemically induced MH - Interleukin-17/metabolism MH - Skin/pathology/drug effects/immunology MH - Disease Models, Animal MH - Acetyl-CoA Carboxylase/antagonists & inhibitors MH - Macrolides PMC - PMC13714554 OTO - NOTNLM OT - acetyl-CoA carboxylase (ACC) 1 OT - fatty acid synthesis OT - metabolic inflammation OT - psoriasis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/28 CRDT- 2026/10/28 03:02 PHST- 2026/08/26 00:00 [revised] PHST- 2026/04/14 00:00 [received] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 03:02 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e70298 [pii] AID - EJI70298 [pii] AID - 10.1002/eji.70298 [doi] PST - ppublish SO - Eur J Immunol. 2026 Oct;56(10):e70298. doi: 10.1002/eji.70298. PMID- 42947522 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1860-7187 (Electronic) IS - 1860-7179 (Print) IS - 1860-7179 (Linking) VI - 21 IP - 21 DP - 2026 Nov 12 TI - The Boron-Neuron Connection: The Potential of Boron-Containing Molecules for Treating Incurable Amyotrophic Lateral Sclerosis. LID - e70554 [pii] LID - 10.1002/cmdc.70554 [doi] AB - BACKGROUND: Boron (B), a group 13 metalloid, has emerged as a versatile element at the intersection of chemistry, biology, and medicine. Its electron-deficient nature and ability to reversibly interconvert between trigonal planar (sp(2)) and tetrahedral (sp(3)) geometries enable dynamic covalent interactions with biological nucleophiles, making boron-containing compounds (BCCs) attractive pharmacophores that selectively interact with reactive oxygen species (ROS) and are ROS-responsive for therapeutic development. BCCs possess antioxidant, anti-inflammatory, and metal-chelating properties that make them attractive candidates for neurodegenerative diseases. Amyotrophic lateral sclerosis (ALS), a fatal and multifactorial motor neurodegenerative disorder, is characterized by oxidative stress, neuroinflammation, mitochondrial dysfunction, and metal dyshomeostasis, providing a strong rationale for exploring boron-based therapeutics. METHODS: This review examines current literature on B chemistry, pharmacology, and translational applications, with emphasis on mechanisms relevant to ALS pathogenesis. This review focuses on ROS-responsive B scaffolds, blood-brain barrier (BBB) permeability, neuroprotective mechanisms, hormonal modulation, metal chelation, and emerging preclinical evidence supporting boron-based interventions. CONCLUSIONS: BCCs represent a promising yet underexplored therapeutic platform for ALS and related neurodegenerative disorders. Their ROS-responsive, redox-modulating, and neuroprotective properties, combined with favorable CNS drug-like pharmacokinetics, offer a mechanistically distinct approach to disease modification. Emerging preclinical evidence strongly supports further translational investigation. CI - (c) 2026 The Author(s). ChemMedChem published by Wiley-VCH GmbH. FAU - Sanghai, Nitesh AU - Sanghai N AUID- ORCID: 0000-0001-5716-3992 AD - College of Pharmacy, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada. FAU - Vuong, Billy AU - Vuong B AUID- ORCID: 0009-0006-1026-5661 AD - College of Pharmacy, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada. FAU - Afridi, Muhammad Shaharyar K AU - Afridi MSK AUID- ORCID: 0000-0001-7251-0024 AD - College of Pharmacy, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada. FAU - Leggett, Sasha AU - Leggett S AUID- ORCID: 0009-0009-5666-8265 AD - College of Pharmacy, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada. FAU - Roghzai, Hemin AU - Roghzai H AUID- ORCID: 0000-0002-3919-061X AD - College of Pharmacy, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada. FAU - Tranmer, Geoffrey K AU - Tranmer GK AUID- ORCID: 0000-0002-9774-8685 AD - College of Pharmacy, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada. LA - eng GR - 202210PJT-495295/Canadian Institutes of Health Research (CIHR)/ GR - RGPIN-2017-05938/Natural Sciences and Engineering Research Council of Canada (NSERC)/ PT - Journal Article PT - Review PL - Germany TA - ChemMedChem JT - ChemMedChem JID - 101259013 RN - 0 (Reactive Oxygen Species) RN - 0 (Neuroprotective Agents) RN - 0 (Boron Compounds) RN - N9E3X5056Q (Boron) SB - IM MH - Humans MH - *Amyotrophic Lateral Sclerosis/drug therapy/pathology/metabolism MH - Reactive Oxygen Species/metabolism MH - Animals MH - *Neuroprotective Agents/chemistry/pharmacology/therapeutic use MH - *Boron Compounds/chemistry/pharmacology/therapeutic use MH - *Neurons/drug effects/metabolism MH - Blood-Brain Barrier/metabolism/drug effects MH - *Boron/chemistry/pharmacology MH - Molecular Structure MH - Oxidative Stress/drug effects PMC - PMC13714559 OTO - NOTNLM OT - amyotrophic lateral sclerosis (ALS) OT - blood-brain barrier (BBB) OT - boron (B) OT - boron-containing compounds (BCCs) OT - neurodegeneration OT - reactive oxygen species (ROS) COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/28 CRDT- 2026/10/28 02:54 PHST- 2026/10/02 00:00 [revised] PHST- 2026/07/27 00:00 [received] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 02:54 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e70554 [pii] AID - CMDC70554 [pii] AID - 10.1002/cmdc.70554 [doi] PST - ppublish SO - ChemMedChem. 2026 Nov 12;21(21):e70554. doi: 10.1002/cmdc.70554. PMID- 42947510 OWN - NLM STAT- Publisher LR - 20261028 IS - 1532-0979 (Electronic) IS - 0147-5185 (Linking) DP - 2026 Oct 28 TI - Endometrioid Carcinoma With Malignant Squamous Differentiation: Clinicopathologic, Immunohistochemical, and Molecular Analysis. LID - 10.1097/PAS.0000000000002641 [doi] AB - Endometrioid carcinoma with squamous differentiation (SqD) is morphologically heterogeneous, and the clinical significance of malignant SqD remains poorly defined. This study aimed to characterize the clinicopathologic, immunophenotypic, and molecular features of endometrioid carcinoma with malignant SqD and to explore its potential implications for diagnosis and risk stratification. We retrospectively reviewed 3696 cases of endometrial endometrioid carcinoma diagnosed from January 2020 to March 2025 and identified 9 tumors with malignant SqD based on predefined histologic criteria. All cases underwent centralized slide review, an immunohistochemical panel, and targeted next-generation sequencing. Tumors were staged according to FIGO 2023 and classified into TCGA/ProMisE molecular subtypes. Malignant SqD was rare but strongly associated with adverse pathologic features, including deep myometrial invasion, extensive lymphovascular space invasion, and frequent nodal metastasis. Preoperative biopsy and curettage frequently underestimated or misclassified malignant SqD. The malignant squamous component predominantly occupied invasive fronts and metastatic deposits and showed consistent loss of hormone receptor expression. Molecular profiling revealed heterogeneity across all 4 molecular subtypes, with recurrent alterations in the PI3K/AKT pathway. Because this aggressive phenotype spans diverse molecular subtypes, conventional morphologic evaluation remains indispensable. CI - Copyright (c) 2026 Wolters Kluwer Health, LLC. All rights reserved. FAU - Lu, Linghui AU - Lu L AD - Department of Pathology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai Key Lab of Reproduction and Development, Shanghai Key Lab of Female Reproductive Endocrine Related Diseases. FAU - Ma, Xiaoxi AU - Ma X AD - Department of Pathology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai Key Lab of Reproduction and Development, Shanghai Key Lab of Female Reproductive Endocrine Related Diseases. FAU - Bao, Longlong AU - Bao L AD - Department of Pathology, Fudan University Shanghai Cancer Center. AD - Department of Oncology, Shanghai Medical College of Fudan University. FAU - Zhao, Chenyan AU - Zhao C AD - Department of Pathology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai Key Lab of Reproduction and Development, Shanghai Key Lab of Female Reproductive Endocrine Related Diseases. FAU - Xu, Ying AU - Xu Y AD - Department of Pathology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai Key Lab of Reproduction and Development, Shanghai Key Lab of Female Reproductive Endocrine Related Diseases. FAU - Zhang, He AU - Zhang H AD - Department of Radiology, Pudong Hospital of Fudan University, Shanghai, China. FAU - Ning, Yan AU - Ning Y AD - Department of Pathology, Obstetrics and Gynecology Hospital of Fudan University, Shanghai Key Lab of Reproduction and Development, Shanghai Key Lab of Female Reproductive Endocrine Related Diseases. LA - eng GR - grant numbers 25ZR1401036/Natural Science Foundation of Shanghai/ GR - grant numbers PWZbr2022-16/Discipline Construction Project of Shanghai Pudong New Area Health Commission/ PT - Journal Article DEP - 20261028 PL - United States TA - Am J Surg Pathol JT - The American journal of surgical pathology JID - 7707904 SB - IM OTO - NOTNLM OT - clinicopathologic OT - endometrioid carcinoma OT - malignant squamous differentiation OT - molecular feature OT - treatment COIS- Conflicts of Interest and Source of Funding: This work was supported by ‌the Natural Science Foundation of Shanghai [grant numbers 25ZR1401036] and the Discipline Construction Project of Shanghai Pudong New Area Health Commission [grant numbers PWZbr2022-16]. The authors have disclosed that they have no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 02:52 PHST- 2026/07/16 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 02:52 [entrez] AID - 00000478-990000000-00743 [pii] AID - 10.1097/PAS.0000000000002641 [doi] PST - aheadofprint SO - Am J Surg Pathol. 2026 Oct 28. doi: 10.1097/PAS.0000000000002641. PMID- 42947509 OWN - NLM STAT- Publisher LR - 20261028 IS - 1096-9896 (Electronic) IS - 0022-3417 (Linking) DP - 2026 Oct 27 TI - Beyond a single ratio: an adaptable deep learning approach to analyze the prognostic value of TSP distribution in ovarian cancer. LID - 10.1002/path.70142 [doi] AB - The tumor-stroma percentage (TSP) has been proposed as a prognostic factor in ovarian cancer. When assessed manually by pathologists, the TSP can be subject to significant inter-rater variability. Automated methods for TSP determination have recently been developed. Existing approaches typically yield a single TSP value for a relatively small region of interest in the slide, introducing bias through region selection and potentially overlooking the TSP distribution across the entire histologically examined area. We present an adaptable deep learning pipeline, efficiently fine-tuned for ovarian cancer tissue analysis using a few-shot learning approach, requiring only minimal new training annotations. Instead of calculating a single TSP value, our method quantifies the global TSP distribution across the entire tumor and therefore captures its spatial heterogeneity. To identify a prognostic biomarker, we systematically evaluated a panel of metrics describing this distribution in 98 patients. Our analysis revealed that the mean-median difference of the TSP distribution [hazard ratio (HR) = 0.60, 95% CI = 0.46-0.79, p < 0.001], a quantitative measure of asymmetry, together with the mean TSP (HR = 0.65, 95% CI = 0.43-0.97, p = 0.03), are biomarkers significantly associated with recurrence-free survival. By successfully adapting a pipeline for ovarian cancer, we demonstrate that automated analysis of the entire TSP distribution provides significant prognostic information superior to that obtained by manual assessment. Spatial heterogeneity of the TSP is a key prognostic factor. (c) 2026 Fraunhofer IIS and The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland. CI - (c) 2026 Fraunhofer IIS and The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland. FAU - Firmbach, Daniel AU - Firmbach D AUID- ORCID: 0009-0008-4952-0193 AD - Institute of Pathology, Universitatsklinikum Erlangen, Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Erlangen, Germany. AD - Comprehensive Cancer Center Erlangen-EMN, Universitatsklinikum Erlangen, Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Erlangen, Germany. FAU - Kuritcyn, Petr AU - Kuritcyn P AUID- ORCID: 0000-0003-2578-7754 AD - Medical Image Analysis Group, Fraunhofer Institute for Integrated Circuits IIS, Erlangen, Germany. FAU - Benz, Michaela AU - Benz M AUID- ORCID: 0000-0003-0625-4418 AD - Medical Image Analysis Group, Fraunhofer Institute for Integrated Circuits IIS, Erlangen, Germany. FAU - Strissel, Pamela L AU - Strissel PL AD - Institute of Pathology, Universitatsklinikum Erlangen, Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Erlangen, Germany. AD - Comprehensive Cancer Center Erlangen-EMN, Universitatsklinikum Erlangen, Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Erlangen, Germany. AD - Bavarian Cancer Research Center, Erlangen, Germany. AD - Department of Gynecology and Obstetrics, Laboratory for Molecular Medicine, Universitatsklinikum Erlangen, Erlangen, Germany. AD - Adjunct Affiliation with Department of Radiation Oncology, University of Maryland School of Medicine, Baltimore, MD, USA. FAU - Strick, Reiner AU - Strick R AUID- ORCID: 0000-0002-8141-7099 AD - Comprehensive Cancer Center Erlangen-EMN, Universitatsklinikum Erlangen, Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Erlangen, Germany. AD - Bavarian Cancer Research Center, Erlangen, Germany. AD - Department of Gynecology and Obstetrics, Laboratory for Molecular Medicine, Universitatsklinikum Erlangen, Erlangen, Germany. FAU - Beckmann, Matthias W AU - Beckmann MW AD - Comprehensive Cancer Center Erlangen-EMN, Universitatsklinikum Erlangen, Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Erlangen, Germany. AD - Bavarian Cancer Research Center, Erlangen, Germany. AD - Department of Gynecology and Obstetrics, Laboratory for Molecular Medicine, Universitatsklinikum Erlangen, Erlangen, Germany. FAU - Glossner, Laura AU - Glossner L AD - Institute of Pathology, Universitatsklinikum Erlangen, Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Erlangen, Germany. AD - Comprehensive Cancer Center Erlangen-EMN, Universitatsklinikum Erlangen, Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Erlangen, Germany. FAU - Emons, Julius AU - Emons J AUID- ORCID: 0000-0002-2520-4443 AD - Comprehensive Cancer Center Erlangen-EMN, Universitatsklinikum Erlangen, Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Erlangen, Germany. AD - Bavarian Cancer Research Center, Erlangen, Germany. AD - Department of Gynecology and Obstetrics, Laboratory for Molecular Medicine, Universitatsklinikum Erlangen, Erlangen, Germany. FAU - Bruns, Volker AU - Bruns V AUID- ORCID: 0000-0001-7693-3542 AD - Medical Image Analysis Group, Fraunhofer Institute for Integrated Circuits IIS, Erlangen, Germany. FAU - Hartmann, Arndt AU - Hartmann A AUID- ORCID: 0000-0002-4860-4626 AD - Institute of Pathology, Universitatsklinikum Erlangen, Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Erlangen, Germany. AD - Comprehensive Cancer Center Erlangen-EMN, Universitatsklinikum Erlangen, Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Erlangen, Germany. AD - Bavarian Cancer Research Center, Erlangen, Germany. FAU - Eckstein, Markus AU - Eckstein M AUID- ORCID: 0000-0001-5418-3349 AD - Institute of Pathology, Universitatsklinikum Erlangen, Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Erlangen, Germany. AD - Comprehensive Cancer Center Erlangen-EMN, Universitatsklinikum Erlangen, Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Erlangen, Germany. AD - Bavarian Cancer Research Center, Erlangen, Germany. FAU - Matek, Christian AU - Matek C AUID- ORCID: 0000-0001-8528-8581 AD - Institute of Pathology, Universitatsklinikum Erlangen, Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Erlangen, Germany. AD - Comprehensive Cancer Center Erlangen-EMN, Universitatsklinikum Erlangen, Friedrich-Alexander-Universitat Erlangen-Nurnberg (FAU), Erlangen, Germany. AD - Bavarian Cancer Research Center, Erlangen, Germany. LA - eng GR - J101/Interdisciplinary Center for Clinical Research (IZKF) at the University Hospital of Erlangen-Nuremberg/ GR - D36/Interdisciplinary Center for Clinical Research (IZKF) at the University Hospital of Erlangen-Nuremberg/ GR - 01KD2429/Budesministerium fur Forschung, Technologie und Raumfahrt/ PT - Journal Article DEP - 20261027 PL - England TA - J Pathol JT - The Journal of pathology JID - 0204634 SB - IM OTO - NOTNLM OT - biomarker OT - deep learning OT - digital pathology OT - few-shot learning OT - ovarian cancer OT - tissue segmentation OT - tumor-stroma percentage OT - whole slide imaging EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 02:52 PHST- 2026/09/16 00:00 [revised] PHST- 2026/02/25 00:00 [received] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 02:52 [entrez] AID - 10.1002/path.70142 [doi] PST - aheadofprint SO - J Pathol. 2026 Oct 27. doi: 10.1002/path.70142. PMID- 42947501 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2058-7384 (Electronic) IS - 0394-6320 (Linking) VI - 40 DP - 2026 Jan-Dec TI - Infection-related adverse events associated with T-cell-engaging bispecific agents: A real-world pharmacovigilance study using FAERS/AEMS. PG - 3946320261499210 LID - 10.1177/03946320261499210 [doi] AB - BackgroundT-cell-engaging bispecific agents and related T-cell-engaging therapies are increasingly used across hematologic malignancies and selected solid tumors. Infection is a clinically important safety concern, but infection-focused post-marketing comparisons across the current product set remain limited. We evaluated infection-related adverse event reporting for T-cell-engaging agents using the FDA Adverse Event Reporting System/Adverse Event Monitoring System (FAERS/AEMS).MethodsPublic FAERS/AEMS quarterly ASCII files from 2022Q1 to 2026Q1 were analyzed. Reports were deduplicated by retaining the latest case version for each CASEID, and exposure was defined as a target T-cell-engaging agent recorded as the primary suspect drug. Infection-related events were identified with a prespecified MedDRA preferred-term dictionary and were summarized using a structured dominant-category framework. Disproportionality was assessed using reporting odds ratio (ROR) with 95% confidence interval (CI), with proportional reporting ratio (PRR) and information component (IC) as supportive metrics. Reporting followed the READUS-PV guideline for disproportionality analyses of individual case safety reports.ResultsAmong 6,102,203 deduplicated primary-suspect reports, 15,108 involved a target T-cell-engaging agent, of which 3,114 were infection-related (20.6%). The class-level infection-related reporting signal was significant (ROR 2.03 (1.95-2.11); IC025 0.80). The largest number of infection-related reports was observed for Teclistamab (Tecvayli) (829 reports), whereas the highest drug-level ROR was observed for Elranatamab (Elrexfio) (ROR 4.38 (3.94-4.87)). Prominent preferred-term signals included pneumonia (ROR 2.01 (1.84-2.21)), sepsis (ROR 3.76 (3.32-4.24)), septic shock (ROR 4.39 (3.71-5.19)), cytomegalovirus infection reactivation (ROR 27.19 (23.39-31.60)), and cytomegalovirus infection (ROR 12.60 (10.71-14.82)). The class-level signal persisted under the narrower infection definition (2,545 reports; ROR 1.95, 95% CI 1.87-2.03). Concordant DailyMed infection terms and FAERS/AEMS infection signals were found for 7 agents.ConclusionsFAERS/AEMS data showed a class-level reporting signal for infection-related adverse events with T-cell-engaging agents, particularly among BCMA x CD3 and CD20 x CD3 therapies. Cytomegalovirus-related events, pneumonia, and sepsis were prominent signal patterns. These findings are hypothesis-generating reporting patterns and should not be interpreted as incidence estimates, relative risks, or proof of causality. FAU - Liu, Shui AU - Liu S AD - Department of Pharmacy, The fourth affiliated hospital of Nanjing Medical University, Nanjing, China. RINGGOLD: 531845 AD - Department of Pharmacy, The Second People's Hospital of Huai'an, Huai'an, Jiangsu, China. FAU - Tan, Ye AU - Tan Y AUID- ORCID: 0009-0004-4651-4194 AD - Department of Clinical Medicine, Kangda College of Nanjing Medical University, Lianyungang, China. RINGGOLD: 560473 FAU - Chen, Aidong AU - Chen A AD - The Key Laboratory of Targeted Intervention of Cardiovascular Disease, Nanjing Medical University, Nanjing, China. RINGGOLD: 12461 FAU - Liu, Qian AU - Liu Q AD - Department of Pharmacy, The fourth affiliated hospital of Nanjing Medical University, Nanjing, China. RINGGOLD: 531845 LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Int J Immunopathol Pharmacol JT - International journal of immunopathology and pharmacology JID - 8911335 RN - 0 (Antibodies, Bispecific) SB - IM MH - Humans MH - Adverse Drug Reaction Reporting Systems MH - Pharmacovigilance MH - *T-Lymphocytes/immunology/drug effects MH - *Antibodies, Bispecific/adverse effects MH - United States OTO - NOTNLM OT - AEMS OT - FAERS OT - T-cell engager OT - bispecific antibody OT - cytomegalovirus OT - infection OT - pharmacovigilance OT - reporting odds ratio COIS- Declaration of conflicting interestsThe authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 02:43 PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 02:43 [entrez] AID - 10.1177/03946320261499210 [doi] PST - ppublish SO - Int J Immunopathol Pharmacol. 2026 Jan-Dec;40:3946320261499210. doi: 10.1177/03946320261499210. Epub 2026 Oct 27. PMID- 42947494 OWN - NLM STAT- Publisher LR - 20261028 IS - 1460-2431 (Electronic) IS - 0022-0957 (Linking) DP - 2026 Oct 28 TI - Integrating environmental adaptation, breeding, and rhizobia bioinoculants for soybean expansion in Europe. LID - erag539 [pii] LID - 10.1093/jxb/erag539 [doi] AB - Europe's heavy dependence on imported protein is environmentally and strategically costly. Soybean (Glycine max) offers a great opportunity to close this gap. Soybean cultivation can reduce reliance on synthetic nitrogen (N) fertiliser through its symbiosis with N-fixing bacteria (rhizobia). Nevertheless, it remains a relatively new crop across much of Europe and establishing it in the continent's diverse environments raises challenges of climate adaptation, symbiotic N fixation, and crop management. We argue that these challenges must be addressed together rather than separately, because yield stability, N fixation, and environmental stress resilience emerge from the interactions between plant genotype, rhizobial strain, environment, and soil and crop management practices. Drawing on lessons from major soybean-producing regions, we review environmental adaptation through maturity-group selection and stress-resilience breeding, rhizobia inoculant design, and the management practices that shape symbiotic and crop performance. We further examine genetic, genomic, and computational resources that make these interactions predictable and optimisable and identify priorities for future research and practice. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Society for Experimental Biology. FAU - Jacott, Catherine N AU - Jacott CN AUID- ORCID: 0000-0002-4285-0228 AD - Instituto de Bioquimica Vegetal y Fotosintesis, Consejo Superior de Investigaciones Cientificas (CSIC)-University of Seville, 41092, Seville, Spain. AD - Department of Microbiology, Avenida de Reina Mercedes 6, University of Seville, 41012, Seville, Spain. FAU - Wilkinson, Helen AU - Wilkinson H AUID- ORCID: 0000-0001-9441-8763 AD - Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium. AD - VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium. FAU - Lopez-Beltran, Maria AU - Lopez-Beltran M AD - Instituto de Bioquimica Vegetal y Fotosintesis, Consejo Superior de Investigaciones Cientificas (CSIC)-University of Seville, 41092, Seville, Spain. AD - Department of Microbiology, Avenida de Reina Mercedes 6, University of Seville, 41012, Seville, Spain. FAU - Rotundo, Jose L AU - Rotundo JL AUID- ORCID: 0000-0003-1675-4600 AD - Corteva Agriscience, at Ctra. Sevilla Cazalla (C 433), km 4,600, 41309, La Rinconada, Spain. FAU - Couzigou, Jean-Malo AU - Couzigou JM AUID- ORCID: 0000-0002-1362-6286 AD - Laboratoire de Recherche en Sciences Vegetales, Universite de Toulouse, CNRS, Institut National Polytechnique-AgroToulouse, Castanet-Tolosan 31320, France. FAU - Goormachtig, Sofie AU - Goormachtig S AUID- ORCID: 0000-0001-6195-9889 AD - Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium. AD - VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium. FAU - Del Cerro, Pablo AU - Del Cerro P AUID- ORCID: 0000-0002-8030-7226 AD - Instituto de Bioquimica Vegetal y Fotosintesis, Consejo Superior de Investigaciones Cientificas (CSIC)-University of Seville, 41092, Seville, Spain. AD - Department of Microbiology, Avenida de Reina Mercedes 6, University of Seville, 41012, Seville, Spain. FAU - Hungria, Mariangela AU - Hungria M AUID- ORCID: 0000-0002-5132-8685 AD - Soil Biotechnology Laboratory, Embrapa Soja, Caixa Postal 231, CEP 86001-970 Londrina, Parana, Brazil. FAU - Khazaei, Hamid AU - Khazaei H AUID- ORCID: 0000-0002-5202-8764 AD - Natural Resources Institute Finland, Latokartanonkaari 9, Helsinki, 00790, Finland. AD - Department of Agricultural Science, University of Helsinki, Latokartanonkaari 5-7, FI-00014, Helsinki, Finland. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - J Exp Bot JT - Journal of experimental botany JID - 9882906 SB - IM OTO - NOTNLM OT - Glycine max OT - Environmental adaptation OT - grain legumes OT - plant-microbe interactions OT - protein security OT - symbiotic nitrogen fixation OT - yield stability EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 02:43 PHST- 2026/07/14 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 02:43 [entrez] AID - 9003320 [pii] AID - 10.1093/jxb/erag539 [doi] PST - aheadofprint SO - J Exp Bot. 2026 Oct 28:erag539. doi: 10.1093/jxb/erag539. PMID- 42947477 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1521-2254 (Electronic) IS - 1099-498X (Print) IS - 1099-498X (Linking) VI - 28 IP - 11 DP - 2026 Nov TI - RNA-Binding Protein NOVA1 Restrains Immune Escape and Liver Metastasis in Colorectal Cancer via Stabilizing NRXN1 mRNA. LID - e70119 [pii] LID - 10.1002/jgm.70119 [doi] AB - BACKGROUND: Colorectal cancer (CRC) liver metastasis is a primary cause of cancer-related mortality. The occurrence of colorectal liver metastasis (CRLM) is closely associated with tumor immune escape, through which cancer cells evade immune surveillance via multiple mechanisms, thereby promoting metastatic dissemination. RNA-binding proteins (RBPs) play crucial roles in gene expression regulation. Neuro-oncological ventral antigen 1 (NOVA1), an important RBP, is often dysregulated in cancer progression. This study aimed to investigate the molecular mechanisms underlying the role of NOVA1 in CRLM. METHODS: The expression levels of NOVA1 and NRXN1 in CRC tissues and cell lines were detected by quantitative real-time polymerase chain reaction (RT-qPCR) and Western blot. Cell proliferation, migration, and invasion were evaluated using Cell Counting Kit-8 (CCK-8), colony formation assays, Transwell assays and wound healing assays. Flow cytometry was used to analyze CRC cell apoptosis. Subsequently, CRC cells were co-cultured with CD8(+) T cells. Flow cytometry, enzyme-linked immunosorbent assay (ELISA), and lactate dehydrogenase (LDH) release assay were used to measure CD8(+) T-cell proliferation, apoptosis, cytokine secretion (tumor necrosis factor-alpha [TNF-alpha] and interferon-gamma [IFN-gamma]), and cytotoxic activity. RNA immunoprecipitation (RIP), RNA pull-down, and actinomycin D assays were performed to verify the binding of NOVA1 to NRXN1 mRNA and its regulatory effect on mRNA stability. In vivo validation was performed using a mouse liver metastasis model. Liver metastasis was observed monitored by live imaging, and liver metastatic foci were quantified using hematoxylin and eosin (H&E) staining. The expression of Ki-67, CD8, and programmed death-ligand 1 (PD-L1) in tumor tissues was detected by immunohistochemistry (IHC) and immunofluorescence (IF). RESULTS: NOVA1 expression was downregulated in CRC tissues and cell lines. Functional assays demonstrated that NOVA1 overexpression inhibited CRC cell proliferation (colony formation reduced by approximately 50%), migration (migrated cells reduced by approximately 40%), and immune escape (CD8(+) T cell proliferation increased by approximately 45%) in vitro, whereas NOVA1 knockdown promoted these processes. RIP and mRNA stability assays revealed that NOVA1 binds to NRXN1 mRNA (5.16-fold enrichment over IgG) and enhances its stability (a 30% increase relative to the LV-NC group), thereby upregulating NRXN1 expression. Rescue experiments showed that NRXN1 knockdown reversed the inhibitory effects of NOVA1 overexpression on CRC cell immune escape (evidenced by a 55% decrease in CD8(+) T cell proliferation) and malignant progression (colony formation and migrated cells increased by approximately 40%-50%). In vivo experiments confirmed that compared with the control group, NOVA1 overexpression reduced the number of liver metastatic foci by 50%; decreased percentages of Ki-67-positive and PD-L1-positive cells by 40% and 30%, respectively; and increased CD8(+) T cell infiltration by 65%. Conversely, NRXN1 knockdown increased liver metastatic foci by 30%; increased percentages of Ki-67-positive and PD-L1-positive cells by 40% and 30%, respectively; and reduced CD8(+) T cell infiltration by 45%. CONCLUSION: NOVA1 influences the immune escape, proliferation, and migration of CRC cells by directly binding to NRXN1 mRNA and regulating its mRNA stability. This study reveals the key regulatory role of the NOVA1-NRXN1 axis in CRLM, suggesting that this axis may serve as a potential therapeutic target and prognostic biomarker. Targeting the NOVA1-NRXN1 signaling pathway may provide a novel strategy for clinical intervention in CRLM. CI - (c) 2026 The Author(s). The Journal of Gene Medicine published by John Wiley & Sons Ltd. FAU - Wei, ZhiXing AU - Wei Z AD - Department of Hepatobiliary Surgery, Heping Hospital Affiliated to Changzhi Medical College, Changzhi City, Shanxi Province, China. FAU - Wei, Xing AU - Wei X AD - Changzhi Medical College, Changzhi City, Shanxi Province, China. FAU - Cui, LinSai AU - Cui L AD - Changzhi Medical College, Changzhi City, Shanxi Province, China. FAU - Pang, YaXin AU - Pang Y AD - Department of Anesthesiology, Heping Hospital Affiliated to Changzhi Medical College, Changzhi City, Shanxi Province, China. FAU - Cui, Guang AU - Cui G AD - Department of Hepatobiliary Surgery, Heping Hospital Affiliated to Changzhi Medical College, Changzhi City, Shanxi Province, China. FAU - Wei, Zhen AU - Wei Z AD - Department of Hepatobiliary Surgery, Heping Hospital Affiliated to Changzhi Medical College, Changzhi City, Shanxi Province, China. FAU - He, ZhaoCai AU - He Z AD - Department of Hepatobiliary Surgery, Heping Hospital Affiliated to Changzhi Medical College, Changzhi City, Shanxi Province, China. FAU - Wu, BuQiang AU - Wu B AUID- ORCID: 0009-0003-4691-756X AD - Department of Hepatobiliary Surgery, Heping Hospital Affiliated to Changzhi Medical College, Changzhi City, Shanxi Province, China. LA - eng PT - Journal Article PL - England TA - J Gene Med JT - The journal of gene medicine JID - 9815764 RN - 0 (Neuro-Oncological Ventral Antigen) RN - 0 (RNA-Binding Proteins) RN - 0 (NOVA1 protein, human) RN - 0 (RNA, Messenger) SB - IM MH - Humans MH - *Colorectal Neoplasms/pathology/genetics/immunology/metabolism MH - Neuro-Oncological Ventral Antigen MH - *Liver Neoplasms/secondary/genetics/immunology/metabolism MH - Animals MH - *RNA-Binding Proteins/genetics/metabolism MH - Mice MH - Gene Expression Regulation, Neoplastic MH - Cell Proliferation/genetics MH - *Tumor Escape/genetics MH - Cell Line, Tumor MH - Male MH - Cell Movement/genetics MH - RNA, Messenger/genetics MH - Female MH - Apoptosis/genetics MH - RNA Stability PMC - PMC13714442 OTO - NOTNLM OT - RNA-binding protein OT - colorectal cancer OT - immune escape OT - liver metastasis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/28 CRDT- 2026/10/28 02:32 PHST- 2026/10/10 00:00 [revised] PHST- 2026/01/06 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 02:32 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e70119 [pii] AID - JGM70119 [pii] AID - 10.1002/jgm.70119 [doi] PST - ppublish SO - J Gene Med. 2026 Nov;28(11):e70119. doi: 10.1002/jgm.70119. PMID- 42947476 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1521-2254 (Electronic) IS - 1099-498X (Print) IS - 1099-498X (Linking) VI - 28 IP - 11 DP - 2026 Nov TI - Identification of a Glycosylation-Related Gene Prognostic Signature for Non-Small Cell Lung Cancer. LID - e70121 [pii] LID - 10.1002/jgm.70121 [doi] AB - PURPOSE: A prognostic signature for non-small cell lung cancer (NSCLC) was developed through identification and integration of glycosylation-related genes (GRGs). METHODS: TCGA RNA-seq data for LUAD and LUSC were analyzed. GRGs were intersected with differentially expressed genes; prognostic GRGs were selected via Cox and LASSO regression to construct a risk model. The immune microenvironment and immunotherapy responses were compared between risk groups. An miRNA-mRNA-transcription factor (TF) network was built and potential drugs were predicted. ADAMTS15 expression was verified by RT-qPCR in NSCLC tissues and cell lines; functional assays (CCK-8, wound healing, Transwell) were performed after ADAMTS15 knockdown in A549 (LUAD) and NCI-H520 (LUSC) cells. RESULTS: A total of 134 differentially expressed GRGs were identified. Ten genes (ADAMTS15, PCSK9, MUC2, B3GNT3, etc.) were selected for the prognostic model, which functioned as an independent prognostic factor. High-risk patients showed poorer immunotherapy response. The regulatory network included 30 TFs and 142 miRNAs, with 107 drug-target pairs predicted. ADAMTS15 was elevated in NSCLC; its knockdown inhibited proliferation, migration, and invasion in both LUAD and LUSC cell models. CONCLUSION: A 10-gene prognostic signature derived from GRGs was identified. Functional validation affirmed ADAMTS15's tumor-promoting role, offering insights for NSCLC prognosis and targeted therapy. CI - (c) 2026 The Author(s). The Journal of Gene Medicine published by John Wiley & Sons Ltd. FAU - Zhao, Qingtao AU - Zhao Q AUID- ORCID: 0009-0006-7677-6915 AD - Department of Thoracic Surgery, Hebei General Hospital, Shijiazhuang, Hebei, China. FAU - Zhang, Xiaopeng AU - Zhang X AUID- ORCID: 0000-0002-4267-1676 AD - Department of Thoracic Surgery, Hebei General Hospital, Shijiazhuang, Hebei, China. FAU - Duan, Guochen AU - Duan G AUID- ORCID: 0000-0001-9608-4066 AD - Department of Thoracic Surgery, Hebei General Hospital, Shijiazhuang, Hebei, China. LA - eng GR - 22377790D/Key Research and Development Program of Hebei Province/ PT - Journal Article PL - England TA - J Gene Med JT - The journal of gene medicine JID - 9815764 RN - 0 (Biomarkers, Tumor) RN - 0 (MicroRNAs) SB - IM MH - Humans MH - *Carcinoma, Non-Small-Cell Lung/genetics/pathology/metabolism/mortality MH - Glycosylation MH - *Lung Neoplasms/genetics/metabolism/pathology/mortality MH - *Gene Expression Regulation, Neoplastic MH - Prognosis MH - *Biomarkers, Tumor/genetics MH - MicroRNAs/genetics MH - Gene Regulatory Networks MH - Female MH - Gene Expression Profiling MH - Cell Line, Tumor MH - Tumor Microenvironment/genetics MH - *Transcriptome PMC - PMC13714441 OTO - NOTNLM OT - NSCLC OT - glycosylation OT - prognostic signature COIS- All authors have accepted responsibility for the contents of this manuscript and approved its submission. The authors declare no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/28 CRDT- 2026/10/28 02:32 PHST- 2026/10/14 00:00 [revised] PHST- 2026/05/19 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 02:32 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e70121 [pii] AID - JGM70121 [pii] AID - 10.1002/jgm.70121 [doi] PST - ppublish SO - J Gene Med. 2026 Nov;28(11):e70121. doi: 10.1002/jgm.70121. PMID- 42947475 OWN - NLM STAT- Publisher LR - 20261028 IS - 1531-8257 (Electronic) IS - 0885-3185 (Linking) DP - 2026 Oct 27 TI - Longitudinal Real-Time Quaking-Induced Conversion Analysis of Misfolded alpha-Synuclein in Idiopathic Rapid Eye Movement Sleep Behavior Disorder Patients with Baseline Cerebrospinal Fluid Negativity. LID - 10.1002/mds.70592 [doi] AB - BACKGROUND: While most patients with idiopathic REM sleep behavior disorder (IRBD) show CSF misfolded alpha-synucleinpositivity, a subset tests negative with an uncertain underlying substrate that may include non-synucleinopathy conditions. OBJECTIVE AND METHODS: We performed a longitudinal study in polysomnography-confirmed IRBD patients with negative CSF alpha-synuclein at baseline (LP1) who underwent a second lumbar puncture (LP2). alpha-synuclein presence was determined by seed amplification assay. RESULTS: Among 43 alphaSyn -negative patients at LP1, 9 (20.9%) converted to alphaSyn positivity over a mean follow-up of 2.8 +/- 2.3 years. At LP1, converters exhibited a higher burden of Parkinson's disease biomarkers, including hyposmia, abnormal dopamine transporter single-photon emission computed tomography (DAT-SPECT), and increased REM sleep electromyographic activity, compared with patients with persistent alphaSyn negativity. Longer intervals between LP1 and LP2 were associated with higher alphaSyn shifting to positivity. CONCLUSION: Serial CSF alphaSyn assessments may identify a very early synucleinopathy stage. This strategy may improve patient selection for disease-modifying trials targeting alphaSyn. (c) 2026 International Parkinson and Movement Disorder Society. CI - (c) 2026 International Parkinson and Movement Disorder Society. FAU - Maya, Gerard AU - Maya G AUID- ORCID: 0000-0002-5025-2615 AD - Neurology Service, Sleep Centre, Hospital Clinic Barcelona, Universitat de Barcelona, IDIBAPS, CIBERNED: CB06/05/0018-ISCIII, Barcelona, Spain. FAU - Montini, Angelica AU - Montini A AD - Neurology Service, Sleep Centre, Hospital Clinic Barcelona, Universitat de Barcelona, IDIBAPS, CIBERNED: CB06/05/0018-ISCIII, Barcelona, Spain. FAU - Ticca, Alice AU - Ticca A AD - IRCCS Istituto delle Scienze Neurologiche di Bologna, Bologna, Italy. AD - Department of Biomedical and Neuromotor Sciences (DiBiNeM), University of Bologna, Bologna, Italy. FAU - Serradell, Monica AU - Serradell M AD - Neurology Service, Sleep Centre, Hospital Clinic Barcelona, Universitat de Barcelona, IDIBAPS, CIBERNED: CB06/05/0018-ISCIII, Barcelona, Spain. FAU - Mammana, Angela AU - Mammana A AD - IRCCS Istituto delle Scienze Neurologiche di Bologna, Bologna, Italy. FAU - Caballero, Noelia AU - Caballero N AD - Neurology Service, Sleep Centre, Hospital Clinic Barcelona, Universitat de Barcelona, IDIBAPS, CIBERNED: CB06/05/0018-ISCIII, Barcelona, Spain. FAU - Ranieri, Agustina AU - Ranieri A AUID- ORCID: 0009-0004-2967-6685 AD - IRCCS Istituto delle Scienze Neurologiche di Bologna, Bologna, Italy. FAU - Gaig, Carles AU - Gaig C AUID- ORCID: 0000-0002-7113-8125 AD - Neurology Service, Sleep Centre, Hospital Clinic Barcelona, Universitat de Barcelona, IDIBAPS, CIBERNED: CB06/05/0018-ISCIII, Barcelona, Spain. FAU - Mastrangelo, Andrea AU - Mastrangelo A AUID- ORCID: 0000-0003-1578-8112 AD - IRCCS Istituto delle Scienze Neurologiche di Bologna, Bologna, Italy. AD - Department of Biomedical and Neuromotor Sciences (DiBiNeM), University of Bologna, Bologna, Italy. FAU - Parchi, Piero AU - Parchi P AUID- ORCID: 0000-0002-9444-9524 AD - IRCCS Istituto delle Scienze Neurologiche di Bologna, Bologna, Italy. AD - Department of Biomedical and Neuromotor Sciences (DiBiNeM), University of Bologna, Bologna, Italy. FAU - Iranzo, Alex AU - Iranzo A AUID- ORCID: 0000-0002-5618-8271 AD - Neurology Service, Sleep Centre, Hospital Clinic Barcelona, Universitat de Barcelona, IDIBAPS, CIBERNED: CB06/05/0018-ISCIII, Barcelona, Spain. LA - eng GR - BBVA-Baselga/ PT - Journal Article DEP - 20261027 PL - United States TA - Mov Disord JT - Movement disorders : official journal of the Movement Disorder Society JID - 8610688 SB - IM OTO - NOTNLM OT - CSF OT - REM sleep behavior disorder OT - RT-Quick OT - lumbar puncture OT - synuclein EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 02:23 PHST- 2026/08/27 00:00 [revised] PHST- 2026/06/21 00:00 [received] PHST- 2026/08/31 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 02:23 [entrez] AID - 10.1002/mds.70592 [doi] PST - aheadofprint SO - Mov Disord. 2026 Oct 27. doi: 10.1002/mds.70592. PMID- 42947470 OWN - NLM STAT- Publisher LR - 20261028 IS - 1552-4175 (Electronic) IS - 1099-8004 (Linking) DP - 2026 Oct 27 TI - Effectiveness of Dynamic Neuromuscular Stabilization Breathing Exercises Combined With Schroth Exercises in the Treatment of Moderate Idiopathic Scoliosis. PG - 10998004261496448 LID - 10.1177/10998004261496448 [doi] AB - Adolescent idiopathic scoliosis (AIS) is a three-dimensional spinal deformity and may impair respiratory function. This study examined whether adding Dynamic Neuromuscular Stabilization (DNS) breathing exercises to Schroth exercises improved pain, Cobb angle, angle of trunk rotation (ATR), respiratory muscle strength, and quality of life. Thirty adolescents were randomized to the Schroth-DNS group (S-DNSG) or Schroth group (SG); 26 completed the study and were analyzed (n = 13 per group). Both groups completed an eight-week Schroth program three times weekly; the S-DNSG additionally received DNS breathing exercises. The Visual Analog Scale (VAS), Cobb angle, ATR, maximal inspiratory and expiratory pressures (MIP and MEP), and Scoliosis Research Society-22 (SRS-22) were assessed at baseline and post-intervention. Between-group differences were evaluated using baseline-adjusted ANCOVA. Both groups improved in outcomes (p < 0.05). After adjustment, VAS and thoracic ATR were lower in the S-DNSG than in the SG (p < 0.001, etap(2) = 0.622; p = 0.010, etap(2) = 0.256, respectively). Lumbar ATR also favored the S-DNSG at the mean baseline value (p = 0.004, etap(2) = 0.318), although this effect varied with baseline lumbar ATR (group x baseline interaction, p = 0.006). No significant adjusted between-group differences were observed for Cobb angle, MIP, MEP, or SRS-22 scores. Both programs improved outcomes over eight weeks. Adding DNS breathing exercises may provide short-term benefits for pain and selected trunk rotation outcomes. Larger trials with longer-term follow-up are needed to confirm these findings. Trial Registration: NCT06682702 (ClinicalTrials.gov), registered on 6 November 2024. FAU - Eyuboglu, Filiz AU - Eyuboglu F AD - Department of Physiotherapy and Rehabilitation, Faculty of Health Sciences, Uskudar University, Istanbul, Turkiye. RINGGOLD: 232990 FAU - Ilhan, Mert AU - Ilhan M AUID- ORCID: 0000-0001-7982-5694 AD - Department of Physiotherapy and Rehabilitation, Faculty of Health Sciences, Uskudar University, Istanbul, Turkiye. RINGGOLD: 232990 FAU - Basardi, Merve AU - Basardi M AD - Department of Physiotherapy and Rehabilitation, Faculty of Health Sciences, Uskudar University, Istanbul, Turkiye. RINGGOLD: 232990 FAU - Kolayli Cerezci, Tuba AU - Kolayli Cerezci T AD - Department of Physiotherapy and Rehabilitation, Faculty of Health Sciences, Uskudar University, Istanbul, Turkiye. RINGGOLD: 232990 LA - eng SI - ClinicalTrials.gov/NCT06682702 PT - Journal Article DEP - 20261027 PL - United States TA - Biol Res Nurs JT - Biological research for nursing JID - 9815758 SB - IM OTO - NOTNLM OT - Schroth exercises OT - adolescent Idiopathic scoliosis OT - dynamic neuromuscular stabilization OT - quality of life OT - respiratory muscle strength EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 02:22 PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 02:22 [entrez] AID - 10.1177/10998004261496448 [doi] PST - aheadofprint SO - Biol Res Nurs. 2026 Oct 27:10998004261496448. doi: 10.1177/10998004261496448. PMID- 42947458 OWN - NLM STAT- Publisher LR - 20261028 IS - 2330-1619 (Electronic) IS - 2330-1619 (Linking) DP - 2026 Oct 27 TI - MUSIC-PD: A Delphi-Derived, Culturally Adaptable Music Therapy Protocol for Parkinson's Disease Rehabilitation. LID - 10.1002/mdc3.70860 [doi] AB - BACKGROUND: Music-based interventions have shown promise in Parkinson's disease (PD) rehabilitation. However, variability in intervention design, delivery, dose, and documentation limits clinical translation. OBJECTIVE: The aim of the study was to develop a consensus-based music therapy protocol for PD, designed to permit cultural and linguistic adaptation. METHODS: A PRISMA-informed evidence-mapping review identified music-based and rhythm-based interventions relevant to PD rehabilitation. Findings informed a modified Delphi process involving a multidisciplinary expert panel of movement disorder neurologists, qualified music therapists, and people with PD. Round 1 generated qualitative inputs on therapeutic domains, safety, feasibility, and cultural adaptation. These were synthesized into 36 structured statements and rated in Round 2 using a 5-point Likert scale. Consensus was predefined as >/=80% agreement. RESULTS: The evidence map included 32 studies and identified 3 therapeutic clusters: motor/gait rehabilitation, voice-respiratory communication, and nonmotor cognitive-affective engagement. Of the 36 Delphi statements, 33 reached consensus. High-convergence domains included patient-preferred familiar music, cultural adaptation, rhythmic auditory stimulation for gait rehabilitation, multidomain symptom targeting, and vocal rehabilitation. The final framework was organized into LAYA-Locomotor Auditory Yielding Activation Module, NADA-Neurocognitive and Affective Domain Activation Module, and RAGA-Respiratory Aerodynamic Glottal Activation Module. The protocol incorporated session-selection rules, safety screening, difficulty levels, active and/or receptive therapy components, flexible pragmatic session guidance, caregiver-supported practice, and a proposed session-planning/documentation resource. CONCLUSION: MUSIC-PD provides a structured, Delphi-derived, and panel-approved framework for integrating music-based interventions into multidisciplinary PD rehabilitation. It translates evidence and multidisciplinary consensus into a protocol intended for prospective feasibility, fidelity, safety, and efficacy evaluation. CI - (c) 2026 International Parkinson and Movement Disorder Society. FAU - Udupa, Mythili AU - Udupa M AUID- ORCID: 0009-0006-4777-2499 AD - School of Music therapy, Institute of Salutogenesis and Complementary Medicine, Sri Balaji Vidyapeeth, Puducherry, India. AD - Parkinson's Disease and Movement Disorders Clinic, Bangalore, India. FAU - R, Sobana AU - R S AUID- ORCID: 0000-0002-6350-4172 AD - School of Music therapy, Institute of Salutogenesis and Complementary Medicine, Sri Balaji Vidyapeeth, Puducherry, India. FAU - Falup-Precurariu, Cristian AU - Falup-Precurariu C AUID- ORCID: 0000-0002-7394-7161 AD - Department of Neurology, Transilvania University, Brasov, Romania. FAU - Mukherjee, Baishali Banerjee AU - Mukherjee BB AUID- ORCID: 0009-0006-7578-8586 AD - School of Music therapy, Institute of Salutogenesis and Complementary Medicine, Sri Balaji Vidyapeeth, Puducherry, India. FAU - J, Jenita Caren Rajakumari AU - J JCR AUID- ORCID: 0000-0001-9038-0948 AD - School of Music therapy, Institute of Salutogenesis and Complementary Medicine, Sri Balaji Vidyapeeth, Puducherry, India. FAU - Chandrasekar, Bhuvaneswari AU - Chandrasekar B AUID- ORCID: 0000-0001-7617-6414 AD - School of Music therapy, Institute of Salutogenesis and Complementary Medicine, Sri Balaji Vidyapeeth, Puducherry, India. FAU - Phielipp, Nicolas Matia AU - Phielipp NM AUID- ORCID: 0000-0002-4631-7551 AD - Department of Neurology, University of California Irvine, USA. FAU - Ojha, Rajeev AU - Ojha R AUID- ORCID: 0000-0001-7680-7036 AD - Department of Neurology, Tribhuvan University Teaching Hospital, Maharajgunj Medical Campus, Kathmandu, Nepal. FAU - Germaine, Chan Hiu Fai AU - Germaine CHF AUID- ORCID: 0000-0001-7059-1107 AD - Department of Medicine, Queen Elizabeth Hospital, Hong Kong SAR. FAU - Ganguly, Jacky AU - Ganguly J AUID- ORCID: 0000-0002-5098-3311 AD - Department of Neurology, Institute of Neuroscience Kolkata, Kolkata, India. FAU - Garg, Divyani AU - Garg D AUID- ORCID: 0000-0003-0532-3370 AD - Department of Neurology, All India Institute of Medical Sciences, New Delhi, India. FAU - Manharlal, Prakash Kumar AU - Manharlal PK AUID- ORCID: 0000-0002-4853-5026 AD - Department of Neurology, Singapore General Hospital, Singapore. AD - National Neuroscience Institute, Singapore. AD - Duke NUS School Of Medicine, Singapore. FAU - Kulkarni, Harish AU - Kulkarni H AD - Program Manager, Capgemeni, and PwP, Bangalore, India. FAU - Caldera, Manjula AU - Caldera M AUID- ORCID: 0000-0001-9034-5341 AD - Department of Neurology, Teaching Hospital Anuradhapura, Sri Lanka. FAU - Sarangamat, Nagaraja AU - Sarangamat N AUID- ORCID: 0009-0008-1613-2928 AD - Department of Neurology, Oxford University Hospitals, Oxford, UK. FAU - Gupta, Rajeev K AU - Gupta RK AD - Aeronautical Society of India, and PwP, India. FAU - Dastidar, Syamal Bhushan Ghosh AU - Dastidar SBG AD - Former Member Railway Board and Ex Officio Secretary to Government of India, and PwP India. FAU - Venkatapathy, Rajagopalan AU - Venkatapathy R AD - Former Bank Employee and PwP, Bangalore, India. FAU - Hegde, Sathyapal AU - Hegde S AUID- ORCID: 0009-0007-8721-1378 AD - Director, Prasanna Technologies Pvt. Ltd. and PwP, Bangalore, India. FAU - Vines, Bradley W AU - Vines BW AUID- ORCID: 0000-0003-4171-0140 AD - Neuroscience, Customer Success, NielsenIQ, California, USA. FAU - Heidersceit, Annie AU - Heidersceit A AUID- ORCID: 0000-0002-8311-7188 AD - Cambridge Institute for Music Therapy Research, Anglia Ruskin University, Cambridge, UK. FAU - Mestre, Tiago AU - Mestre T AUID- ORCID: 0000-0002-6973-7479 AD - Department of Medicine, Division of Neurology, University of Ottawa, Ottawa Hospital, Canada. FAU - Chaudhuri, Kallol Ray AU - Chaudhuri KR AUID- ORCID: 0000-0003-2815-0505 AD - Director of Research and Clinical Trials, King's College Hospital London, Dubai, UAE. AD - Dementech Neuroscience Clinical Academic Centre, London, UK. FAU - Kukkle, Prashanth Lingappa AU - Kukkle PL AUID- ORCID: 0000-0002-4610-947X AD - Parkinson's Disease and Movement Disorders Clinic, Bangalore, India. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Mov Disord Clin Pract JT - Movement disorders clinical practice JID - 101630279 SB - IM PMC - PMC13714363 OTO - NOTNLM OT - Delphi consensus OT - Parkinson's disease OT - music therapy OT - music-based intervention OT - rhythmic auditory stimulation COIS- Ethical Compliance Statement: This Delphi process paper did not require IRB approval. Financial Disclosures and Conflicts of Interest: M.U.: none. S.R.: none. C.F.P. has served on advisory boards for AbbVie; has received honoraria from AbbVie, Boehringer Ingelheim, Organon, Magna pharm, KRKA, and MDS; receives book royalties from Elsevier and Springer; and was awarded a grant from ERA4Health. G.M.P. has consulted for Acadia Pharmaceuticals Inc. and GE Healthcare. B.B.M.: none. C.R.J: none. B.C.: none. N.M.P.: none. R.O. has received travel support to attend annual meeting of Global Parkinson's Genetics Program (GP2) and research grants from Aligning Science Across Parkinson's (ASAP). C.H.F.: none. J.G.: none. D.G. received travel support and honoraria from IPMDS and royalties from JayPee Brothers publishers (FY 2025-2026). P.K.M.: none. H.K.: none. M.C.: none. N.S.: none. R.K.G.: none. S.G.D.: none. R.V.: none. S.H.: none. B.W.V.: none. A.H. none. T.M. has received consulting and Advisory Board Membership with honoraria: AbbVie, MDS, AAN, CHDI Foundation/Management, Roche, UCB, PTC Therapeutics, Novartis, WAVE therapeutics; grants and research: EU Joint Program-Neurodegenerative Disease Research, uOBMRI, Roche, Ontario Research Fund, CIHR, The Michael J. Fox Foundation for Parkinson's Research, Parkinson Canada, Parkinson Research Consortium, and Brain Canada; and salary from UOMA. K.R.C. has been an expert and advisor for AbbVie, Britannia, Neuroderm (clinical trial), and Stada. He has served as an advisory board member for AbbVie, Acadia, Bial, Britannia, Cynapsus, GKC, Lobsor, Medtronic, Profile Pharma, Remepy, Roche, Scion, Stada, Synovion, Theravance, UCB, Zambon, and 4D Pharma. He has received honoraria for lectures from AbbVie, Bial, BI, Britannia, EAN, GKC, Kyowa Kirin, Novartis, MDS, Scion, SK Pharma, UCB, and Zambon. He has received investigator-initiated grants from AbbVie, Bial, Britannia, GKC, and UCB, and academic grants from EU Horizon 2020, Kirby Laing Foundation, MRC, MDS (MDS NMS Project), NIHR, Parkinson's Foundation, Parkinson's UK, and Wellcome Trust. He has received royalties or licenses from Oxford and Cambridge publishers and the MAPI institute. He has received payment for expert testimony from GMC, NICE, and NIHR. P.L.K. has received stipend from The Michael J Fox Foundation, travel grants from IPMDS, and consultancy fees from Medgenome Labs Pvt. Ltd. The authors declare no conflict of interest relevant to this work. Author disclosures are available in the Supporting Information. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 PMCR- 2027/10/27 CRDT- 2026/10/28 02:03 PHST- 2026/09/09 00:00 [revised] PHST- 2026/07/30 00:00 [received] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 02:03 [entrez] PHST- 2027/10/27 00:00 [pmc-release] AID - MDC370860 [pii] AID - 10.1002/mdc3.70860 [doi] PST - aheadofprint SO - Mov Disord Clin Pract. 2026 Oct 27:10.1002/mdc3.70860. doi: 10.1002/mdc3.70860. PMID- 42947452 OWN - NLM STAT- Publisher LR - 20261028 IS - 1557-8070 (Electronic) IS - 1557-8070 (Linking) DP - 2026 Oct 27 TI - Prebiotic Synthesis of L-Homochiral Peptides on Primordial Earth. PG - 15311074261502316 LID - 10.1177/15311074261502316 [doi] AB - The origin of biological homochirality remains a central enigma in astrobiology. The emergence of a homochiral world necessitates two key processes: initial symmetry breaking and subsequent chiral selection and amplification during abiotic polymerization. This study focuses on the latter, specifically investigating whether an initial chiral environment could have influenced the selectivity of prebiotic peptide synthesis. We demonstrate a novel, prebiotically plausible pathway for homochiral peptide formation utilizing amino acid amides (AA-NH(2)) on early Earth. We observed that L-AA-NH(2) environments induce a robust, consistent stereochemical bias, which leads to enriched L-homochiral peptide synthesis. This bias is significantly amplified during oligomerization, achieving near-absolute L-homochirality. D-AA-NH(2) systems, however, exhibited environmentally contingent, fluctuating selectivity. Crucially, peptide formation from Gly-NH(2) in racemic conditions was strongly inhibited, demonstrating the power of subtle chiral induction in driving homochirality. Our results support a model where initial chirality influences the selectivity of peptide polymerization during the origin of life, providing a potential pathway for generating the biological homochirality essential for life and offering new insights into its potential for life elsewhere in the cosmos. FAU - Zhang, Li AU - Zhang L AUID- ORCID: 0009-0008-9807-9840 AD - Institute of Drug Discovery Technology, Qian Xuesen Collaborative Research Center of Astrochemistry and Space Life Sciences, Ningbo University, Ningbo, Zhejiang, China. AD - College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian, China. FAU - Lei, Xiangmin AU - Lei X AUID- ORCID: 0000-0003-4283-1868 AD - College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, China. FAU - Zheng, Xiangxiao AU - Zheng X AUID- ORCID: 0009-0009-1796-8011 AD - Institute of Drug Discovery Technology, Qian Xuesen Collaborative Research Center of Astrochemistry and Space Life Sciences, Ningbo University, Ningbo, Zhejiang, China. FAU - Chen, Fude AU - Chen F AUID- ORCID: 0009-0002-8785-2742 AD - Institute of Drug Discovery Technology, Qian Xuesen Collaborative Research Center of Astrochemistry and Space Life Sciences, Ningbo University, Ningbo, Zhejiang, China. FAU - Yu, Shichao AU - Yu S AUID- ORCID: 0009-0001-7930-5688 AD - Institute of Drug Discovery Technology, Qian Xuesen Collaborative Research Center of Astrochemistry and Space Life Sciences, Ningbo University, Ningbo, Zhejiang, China. FAU - Zhang, Min AU - Zhang M AUID- ORCID: 0000-0001-7683-1802 AD - College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian, China. FAU - Guo, Xiaofan AU - Guo X AUID- ORCID: 0009-0001-8397-3734 AD - College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian, China. FAU - Cai, Huahuan AU - Cai H AD - Institute of Drug Discovery Technology, Qian Xuesen Collaborative Research Center of Astrochemistry and Space Life Sciences, Ningbo University, Ningbo, Zhejiang, China. FAU - Zhao, Yufen AU - Zhao Y AUID- ORCID: 0000-0003-3560-435X AD - Institute of Drug Discovery Technology, Qian Xuesen Collaborative Research Center of Astrochemistry and Space Life Sciences, Ningbo University, Ningbo, Zhejiang, China. AD - College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian, China. FAU - Ying, Jianxi AU - Ying J AUID- ORCID: 0000-0002-1035-5345 AD - Institute of Drug Discovery Technology, Qian Xuesen Collaborative Research Center of Astrochemistry and Space Life Sciences, Ningbo University, Ningbo, Zhejiang, China. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Astrobiology JT - Astrobiology JID - 101088083 SB - IM OTO - NOTNLM OT - Origin of life-Primordial Earth-Prebiotic peptide synthesis-Chiral selection-Amino acid amides EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 02:03 PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 02:03 [entrez] AID - 10.1177/15311074261502316 [doi] PST - aheadofprint SO - Astrobiology. 2026 Oct 27:15311074261502316. doi: 10.1177/15311074261502316. PMID- 42947444 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1612-1880 (Electronic) IS - 1612-1872 (Print) IS - 1612-1872 (Linking) VI - 23 IP - 10 DP - 2026 Oct TI - Synthetic Strategy to Novel Highly Functionalized Furan Analogues Bearing Imidazole-Thiazole Hybrids: Structural Characterization, Nanoparticle Formulations, Antimicrobial Evaluation, Molecular Docking Studies, and Molecular Dynamic Simulations. LID - e71824 [pii] LID - 10.1002/cbdv.71824 [doi] AB - This study reports design and synthesis of novel highly functionalized furan analogues bearing imidazole-thiazole hybrids with potential antimicrobial activity. The synthesized compounds were prepared and characterized using spectroscopic tools and elemental analyses. The antimicrobial activity of all the synthesized compounds was evaluated against a panel of bacterial strains vs ciprofloxacin as the positive control. The results revealed a highly structure- and strain-dependent profile. However, compound 9b emerged as the most potent activity. To enhance the physicochemical and biological performance, selected bioactive compounds (6b, 8a,c; 9b,c; and 11a) were formulated into nanoparticles and characterized, performing acceptable sizes, stability and distribution profiles, while 9b exhibited the favorable parameters. The antimicrobial evaluation for the 6-nanoformulations was assessed and compared with their parent compounds. Nano-compounds 9b > 9c > 8c demonstrated the most potent activity, and lowest MIC values, comparable and even superior to ciprofloxacin in several cases. Molecular docking studies and molecular dynamic simulations were conducted to 9b to get a detailed view including its proposed binding behavior, interaction patterns, conformational rearrangements, and stability within the DNA gyrase B active site compared with that of novobiocin. The findings validate 9b as the most promising candidate for further development as a DNA gyrase B inhibitor. CI - (c) 2026 The Author(s). Chemistry & Biodiversity published by Wiley-VHCA AG. FAU - Sediek, Ashraf A AU - Sediek AA AUID- ORCID: 0000-0002-7090-8642 AD - Chemical Industries research Institute, National Research Centre, Giza, Egypt. FAU - Kamel, Azza A AU - Kamel AA AD - Chemical Industries research Institute, National Research Centre, Giza, Egypt. FAU - El-Borady, Ola M AU - El-Borady OM AD - Institute of Nanoscience and Nanotechnology, Kafrelsheikh University, Kafr ElSheikh, Egypt. FAU - El-Sayed, Ahmed F AU - El-Sayed AF AUID- ORCID: 0000-0002-5312-4870 AD - Microbial Genetics Department, Biotechnology Research Institute, National Research Centre, Giza, Egypt. AD - Egypt Center for Research and Regenerative Medicine (ECRRM), Cairo, Egypt. FAU - El-Rashedy, Ahmed A AU - El-Rashedy AA AUID- ORCID: 0000-0003-3899-5376 AD - Chemistry of Natural and Microbial Products Department, National Research Center (NRC), Giza, Egypt. FAU - Sabry, Eman AU - Sabry E AUID- ORCID: 0009-0000-7826-7408 AD - Chemical Industries research Institute, National Research Centre, Giza, Egypt. LA - eng PT - Journal Article PL - Switzerland TA - Chem Biodivers JT - Chemistry & biodiversity JID - 101197449 RN - 0 (Imidazoles) RN - 0 (Thiazoles) RN - 0 (Furans) RN - 0 (Anti-Bacterial Agents) RN - EC 5.99.1.3 (DNA Gyrase) RN - 7GBN705NH1 (imidazole) SB - IM MH - *Imidazoles/chemistry/pharmacology MH - *Thiazoles/chemistry/pharmacology MH - *Molecular Docking Simulation MH - *Nanoparticles/chemistry MH - Microbial Sensitivity Tests MH - *Furans/chemistry/pharmacology/chemical synthesis MH - *Molecular Dynamics Simulation MH - Structure-Activity Relationship MH - *Anti-Bacterial Agents/pharmacology/chemistry/chemical synthesis MH - DNA Gyrase/metabolism/chemistry MH - Molecular Structure PMC - PMC13714438 OTO - NOTNLM OT - DNA gyrase B OT - antimicrobial activity OT - furan-imidazole-thiazole hybrids OT - molecular docking OT - molecular dynamic simulations OT - nanoparticle formulations COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/28 CRDT- 2026/10/28 01:53 PHST- 2026/09/11 00:00 [revised] PHST- 2026/07/14 00:00 [received] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:53 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e71824 [pii] AID - CBDV71824 [pii] AID - 10.1002/cbdv.71824 [doi] PST - ppublish SO - Chem Biodivers. 2026 Oct;23(10):e71824. doi: 10.1002/cbdv.71824. PMID- 42947437 OWN - NLM STAT- Publisher LR - 20261028 IS - 1477-3848 (Electronic) IS - 0743-4618 (Linking) DP - 2026 Oct 27 TI - Aided language and the demands of simultaneous processing: a case of bimodality? PG - 1-9 LID - 10.1080/07434618.2026.2748032 [doi] AB - For individuals who use graphic-based augmentative and alternative communication (AAC), language production is never a single-channel process. These individuals receive language input through speech while encoding communicative intentions through a graphic symbol system, an asymmetry that differs fundamentally from typical spoken language development. This forum paper proposes that graphic-based AAC functions as a bimodal production system. We draw on Levelt's model of speech production and Emmorey et al.'s extension for bimodal bilinguals. What has been characterized as impoverished language output in aided AAC, we argue, may instead reflect the simultaneous activation of two parallel language formulators, one verbal and one graphic, each routing output to independent articulatory systems under fundamentally different constraints. The graphic formulator operates without the automaticity of typical speech production, requiring overt symbol selection, display navigation, and deliberate morphological encoding through processes such as post-morphing. This framework reframes documented patterns in AAC output, including morphological omission and reduced syntactic complexity, as consequences of a constrained graphic formulator operating in parallel with a verbal formulator that may have generated a more complete linguistic representation. The framework also offers a foundation for investigating bilingual AAC use and future psycholinguistic studies of aided language production. FAU - Soto, Gloria AU - Soto G AUID- ORCID: 0000-0002-3254-3099 AD - Department of Communication Sciences and Disorders, The Pennsylvania State University, State College, USA. FAU - Covarrubias-Alvarez, Maira AU - Covarrubias-Alvarez M AUID- ORCID: 0009-0009-3253-1053 AD - Department of Communication Sciences and Disorders, The Pennsylvania State University, State College, USA. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Augment Altern Commun JT - Augmentative and alternative communication (Baltimore, Md. : 1985) JID - 8504574 SB - IM OTO - NOTNLM OT - Bimodality OT - graphic-based AAC OT - language formulation OT - post-morphing EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 01:53 PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:53 [entrez] AID - 10.1080/07434618.2026.2748032 [doi] PST - aheadofprint SO - Augment Altern Commun. 2026 Oct 27:1-9. doi: 10.1080/07434618.2026.2748032. PMID- 42947435 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1932-8737 (Electronic) IS - 0160-9289 (Print) IS - 0160-9289 (Linking) VI - 49 IP - 10 DP - 2026 Oct TI - Epicardial Adipose Tissue as a Dynamic Modulator of Coronary Artery Disease Phenotypes: Pathophysiology and Therapeutic Implications. LID - e70509 [pii] LID - 10.1002/clc.70509 [doi] AB - BACKGROUND: Epicardial adipose tissue (EAT) represents a localized visceral adipose depot uniquely situated between the myocardium and the visceral layer of the pericardium. Given its proximity to the coronary vasculature and distinct transcriptomic and secretory profile, EAT has been associated with the extent and severity of coronary artery disease (CAD). HYPOTHESIS: Systemic therapeutic interventions may affect distinct CAD phenotypes through modulation of EAT characteristics. METHODS: This review integrates evidence on EAT pathophysiology and therapeutic modulation in relation to distinct CAD phenotypes. A structured search of PubMed, EMBASE, and the Cochrane Library was conducted using combinations of terms related to EAT, distinct CAD phenotypes, and lifestyle, surgical, and pharmacological interventions. Studies were selected based on their relevance to the review objectives. RESULTS: EAT has been implicated in a broad spectrum of CAD phenotypes, ranging from microvascular dysfunction to the development of high-risk atherosclerotic plaque. Advanced imaging modalities enable depot-specific characterization of EAT as an objectively measurable biomarker. Lifestyle interventions, bariatric surgery, and several pharmacological classes, including glucagon-like peptide-1 receptor agonists, sodium-glucose cotransporter-2 inhibitors, lipid-lowering agents, and anti-inflammatory therapies, have been reported to modify EAT. CONCLUSIONS: EAT represents a potential therapeutic target across CAD phenotypes. However, whether therapeutic modification of EAT independently improves cardiovascular outcomes remains uncertain. CI - (c) 2026 The Author(s). Clinical Cardiology published by Wiley Periodicals LLC. FAU - Dormiani Tabatabaei, Seyed Amirhossein AU - Dormiani Tabatabaei SA AUID- ORCID: 0000-0001-9398-5889 AD - Isfahan Cardiovascular Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran. FAU - Dormiani Tabatabaei, Mehrnazsadat AU - Dormiani Tabatabaei M AUID- ORCID: 0009-0001-4704-505X AD - School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. LA - eng PT - Journal Article PT - Review PL - United States TA - Clin Cardiol JT - Clinical cardiology JID - 7903272 SB - IM MH - Humans MH - *Epicardial Adipose Tissue/physiopathology MH - *Coronary Artery Disease/physiopathology/therapy/diagnosis MH - Phenotype MH - Animals MH - *Pericardium/physiopathology PMC - PMC13714447 OTO - NOTNLM OT - adipose tissue OT - atherosclerosis OT - cardiovascular agents OT - coronary artery disease OT - epicardial OT - inflammation COIS- The authors declare that they have no competing interests associated with this manuscript. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/27 CRDT- 2026/10/28 01:53 PHST- 2026/10/08 00:00 [revised] PHST- 2026/05/12 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:53 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e70509 [pii] AID - CLC70509 [pii] AID - 10.1002/clc.70509 [doi] PST - ppublish SO - Clin Cardiol. 2026 Oct;49(10):e70509. doi: 10.1002/clc.70509. PMID- 42947433 OWN - NLM STAT- Publisher LR - 20261028 IS - 1096-9896 (Electronic) IS - 0022-3417 (Linking) DP - 2026 Oct 27 TI - Molecular analysis of matched PIN, prostate cancer, and adjacent normal prostate tissue reveals distinct transcriptional signatures and recurrent genomic alterations. LID - 10.1002/path.70143 [doi] AB - Prostate intraepithelial neoplasia (PIN) is a recognized precursor to prostate cancer (PCa) and shares many histological and molecular features with invasive disease. To clarify the relationship between PIN and PCa, we performed integrated genomic and transcriptomic analyses on epithelial and stromal laser-capture microdissected samples in matched PIN (n = 121), PCa (n = 217), and adjacent normal (AN, n = 115) prostate tissue from formalin-fixed paraffin-embedded (FFPE) samples across three datasets. We identified two epithelial and two stromal transcriptomic clusters, termed 'invasive-like' and 'normal-like', which were present across all datasets. Among PIN samples, 46% of epithelial and 38% of stromal samples were classified as invasive-like. Whole-genome sequencing analysis revealed recurrent copy number alterations in PIN and PCa lesions, with recurrent copy number alterations more frequently shared between invasive-like PIN and PCa. This study provides new insight into PIN biology and the relationship between PIN and invasive PCa and highlights a transcriptional signature in PIN samples that may have prognostic implications. (c) 2026 The Pathological Society of Great Britain and Ireland. CI - (c) 2026 The Pathological Society of Great Britain and Ireland. FAU - Strand, Siri H AU - Strand SH AUID- ORCID: 0000-0001-6596-9945 AD - Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA. AD - Department of Molecular Medicine (MOMA), Aarhus University Hospital, Aarhus, Denmark. AD - Department of Pathology, Oslo University Hospital, Oslo, Norway. FAU - Eminaga, Okyaz AU - Eminaga O AUID- ORCID: 0000-0003-0861-3138 AD - Department of Urology, Stanford University School of Medicine, Stanford, CA, USA. FAU - Kunder, Christian A AU - Kunder CA AD - Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA. FAU - Rasmussen, Martin AU - Rasmussen M AUID- ORCID: 0000-0001-9077-7857 AD - Department of Molecular Medicine (MOMA), Aarhus University Hospital, Aarhus, Denmark. FAU - Qin, Xiaodi AU - Qin X AD - Duke Cancer Institute, Duke University School of Medicine, Durham, NC, USA. FAU - Rogers, Layne W AU - Rogers LW AUID- ORCID: 0000-0002-4881-5589 AD - Duke Cancer Institute, Duke University School of Medicine, Durham, NC, USA. FAU - Vennam, Sujay AU - Vennam S AD - Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA. FAU - Zhu, Chunfang AU - Zhu C AD - Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA. FAU - Wang, Jason AU - Wang J AD - Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA. FAU - Nolley, Rosalie AU - Nolley R AD - Department of Urology, Stanford University School of Medicine, Stanford, CA, USA. FAU - Varma, Sushama AU - Varma S AD - Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA. FAU - Kristensen, Gitte Hjartbro AU - Kristensen GH AUID- ORCID: 0000-0003-0093-9942 AD - Urological Research Unit, Copenhagen Prostate Cancer Center, Center for Cancer and Organ diseases, Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark. AD - Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark. FAU - Roeder, Andreas AU - Roeder A AUID- ORCID: 0000-0002-0019-5333 AD - Urological Research Unit, Copenhagen Prostate Cancer Center, Center for Cancer and Organ diseases, Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark. AD - Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark. FAU - Pollack, Jonathan R AU - Pollack JR AUID- ORCID: 0000-0002-0212-7721 AD - Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA. FAU - Sorensen, Karina Dalsgaard AU - Sorensen KD AUID- ORCID: 0000-0002-4902-5490 AD - Department of Molecular Medicine (MOMA), Aarhus University Hospital, Aarhus, Denmark. FAU - Brooks, James D AU - Brooks JD AUID- ORCID: 0000-0003-4521-7505 AD - Department of Urology, Stanford University School of Medicine, Stanford, CA, USA. FAU - West, Robert B AU - West RB AD - Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA. LA - eng GR - R288-2018-35/Lundbeck Foundation/ GR - R229-A13616/Kraeftens Bekaempelse/ GR - U01 CA196387-01/CA/NCI NIH HHS/United States PT - Journal Article DEP - 20261027 PL - England TA - J Pathol JT - The Journal of pathology JID - 0204634 SB - IM OTO - NOTNLM OT - cancer OT - copy number variations OT - epithelium OT - intraepithelial neoplasia OT - laser capture microdissection OT - precancer OT - prostate OT - stroma OT - tissue microarray OT - transcriptional signature EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 01:53 PHST- 2026/09/15 00:00 [revised] PHST- 2026/03/16 00:00 [received] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:53 [entrez] AID - 10.1002/path.70143 [doi] PST - aheadofprint SO - J Pathol. 2026 Oct 27. doi: 10.1002/path.70143. PMID- 42947422 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1471-2954 (Electronic) IS - 0962-8452 (Linking) VI - 293 IP - 2081 DP - 2026 Oct 28 TI - Hybrid species evolution between cryptic and sympatric weevil species. LID - 20261745 [pii] LID - 10.1098/rspb.2026.1745 [doi] AB - Hybridization may generate a variety of evolutionary outcomes, including novel hybrid species without a change in ploidy. Within this process, referred to as homoploid hybrid speciation (HHS), selection on recombinant traits is often invoked as the driver of reproductive isolation (RI) between hybrid and parental species. However, our understanding of the temporal, geographical and phenotypic dimensions within which hybrid populations evolve RI against parental species is still limited. Here, we describe speciation following hybridization within a beetle species complex that presents no obvious phenotypical or ecological differences among hybrid and parental species, and where all occur sympatrically. Demographic modelling supports hybrid species origin, where the hybrid lineage originated from historical admixture between parental lineages. Genome-wide estimates of heterozygosity and individual-based simulations of hybrid species origin provide further support for an ancient, rather than recent origin of the hybrid species. We use geographical patterns of genomic relatedness between hybrids and parentals to identify the probable origin of the hybrid species within a hybrid swarm that was geographically isolated from both parental species. We then discuss our results within the context of HHS theory to highlight predictions for an HHS origin that can be tested when an appropriate reference genome becomes available. CI - (c) 2026 The Authors. FAU - Noguerales, Victor AU - Noguerales V AUID- ORCID: 0000-0003-3185-778X AD - Institute of Natural Products and Agrobiology (IPNA-CSIC) , San Cristobal de La Laguna, Spain. AD - Instituto de Investigacion en Recursos Cinegeticos (IREC - CSIC, UCLM, JCCM) , Ciudad Real, Spain. FAU - Eisele, Marius H AU - Eisele MH AD - Institute of Natural Products and Agrobiology (IPNA-CSIC) , San Cristobal de La Laguna, Spain. FAU - Gray, Sophia L AU - Gray SL AD - Department of Ecology and Evolution, University of Sussex , Brighton, UK. FAU - Stankowski, Sean AU - Stankowski S AD - Department of Genetics, Evolution, and Environment, University College London , London, UK. FAU - Emerson, Brent C AU - Emerson BC AD - Institute of Natural Products and Agrobiology (IPNA-CSIC) , San Cristobal de La Laguna, Spain. LA - eng GR - CGL2017-85718-P/MCIN/AEI/10.13039/501100011033/ GR - PID2020-116788GB-I00/MCIN/AEI/10.13039/501100011033/ GR - FEDER/ GR - RYC2024-048821-I/Ramon y Cajal/ GR - MICIU/AEI/10.13039/501100011033/ GR - FSE+/ GR - Viera y Clavijo/ GR - Agencia Canaria de Investigacion, Innovacion y Sociedad de la Informacion/ GR - Universidad de La Laguna/ PT - Journal Article PL - England TA - Proc Biol Sci JT - Proceedings. Biological sciences JID - 101245157 SB - IM MH - Animals MH - *Hybridization, Genetic MH - *Weevils/genetics/physiology MH - *Genetic Speciation MH - Sympatry MH - Reproductive Isolation MH - *Biological Evolution OTO - NOTNLM OT - coleoptera OT - homoploid hybrid speciation OT - hybridization OT - insect OT - reproductive isolation OT - speciation EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 01:43 PHST- 2025/11/14 00:00 [received] PHST- 2026/09/01 00:00 [revised] PHST- 2026/09/02 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:43 [entrez] AID - 483938 [pii] AID - 10.1098/rspb.2026.1745 [doi] PST - ppublish SO - Proc Biol Sci. 2026 Oct 28;293(2081):20261745. doi: 10.1098/rspb.2026.1745. PMID- 42947418 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1471-2954 (Electronic) IS - 0962-8452 (Linking) VI - 293 IP - 2081 DP - 2026 Oct 28 TI - Artificial light at night alters the relative dominance of invasive plants in a nutrient-dependent manner across native species richness levels. LID - 20260507 [pii] LID - 10.1098/rspb.2026.0507 [doi] AB - Non-native plant invasions pose a global challenge, yet how nutrient enrichment and artificial light at night (ALAN) interact with native-community species richness to shape invasive-plant performance and community-level dominance remains unclear. We conducted a multispecies greenhouse experiment in which target invasive species and native communities were established simultaneously under fully crossed factorial combinations of ALAN (No-ALAN or ALAN), nutrient enrichment (low or high) and native species richness (two, four or eight species). We quantified target invasive-plant above-ground biomass, flowering probability and proportional above-ground biomass, as well as native-community and total-community above-ground biomass. Nutrient enrichment increased invader above-ground biomass by 272.88%, flowering probability by 30.39% and native-community above-ground biomass by 97.58%, although its effect on proportional invader biomass remained uncertain. ALAN did not clearly affect invader biomass or flowering but increased its proportional biomass under high-nutrient conditions by 5.74% and reduced it under low nutrients by 4.75%. Native species richness showed no clear effects on invader performance, community biomass or responses to ALAN and nutrients. Within this experimental context, invasive-plant performance was driven primarily by nutrient enrichment, with ALAN facilitating invader dominance under high-nutrient conditions, indicating that invasion outcomes were more influenced by resource availability than by native-community species richness. CI - (c) 2026 The Authors. FAU - Zhang, Haobin AU - Zhang H AD - Jilin Da'an Agro-ecosystem National Observation and Research Station, Key Laboratory of Marsh Wetland Ecosystem Conservation and Restoration, National Forestry and Grassland Administration, State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences , Changchun, Jilin, People's Republic of China. FAU - Oduor, Ayub M O AU - Oduor AMO AUID- ORCID: 0000-0001-6714-5253 AD - Jilin Da'an Agro-ecosystem National Observation and Research Station, Key Laboratory of Marsh Wetland Ecosystem Conservation and Restoration, National Forestry and Grassland Administration, State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences , Changchun, Jilin, People's Republic of China. AD - Department of Biological and Life Sciences, Technical University of Kenya , Nairobi, Kenya. AD - Shandong Key Laboratory of Eco-Environmental Science for Yellow River Delta, Shandong University of Aeronautics , Binzhou, Shandong, People's Republic of China. FAU - Xiao, Lu AU - Xiao L AUID- ORCID: 0000-0001-7155-6709 AD - Jilin Da'an Agro-ecosystem National Observation and Research Station, Key Laboratory of Marsh Wetland Ecosystem Conservation and Restoration, National Forestry and Grassland Administration, State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences , Changchun, Jilin, People's Republic of China. FAU - Liu, Yanjie AU - Liu Y AUID- ORCID: 0000-0003-3948-1246 AD - Jilin Da'an Agro-ecosystem National Observation and Research Station, Key Laboratory of Marsh Wetland Ecosystem Conservation and Restoration, National Forestry and Grassland Administration, State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences , Changchun, Jilin, People's Republic of China. LA - eng GR - W2411021/National Natural Science Foundation of China/ GR - 32571783/National Natural Science Foundation of China/ GR - 2026PVB0077/Chinese Academy of Sciences President's International Fellowship Initiative (CAS-PIFI)/ PT - Journal Article PL - England TA - Proc Biol Sci JT - Proceedings. Biological sciences JID - 101245157 SB - IM MH - *Introduced Species MH - *Light MH - *Biodiversity MH - Biomass MH - Nutrients OTO - NOTNLM OT - artificial light at night OT - fluctuating resource hypothesis OT - native-community species richness OT - non-native plant invasion OT - nutrient enrichment EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 01:43 PHST- 2026/02/27 00:00 [received] PHST- 2026/08/19 00:00 [revised] PHST- 2026/08/19 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:43 [entrez] AID - 483934 [pii] AID - 10.1098/rspb.2026.0507 [doi] PST - ppublish SO - Proc Biol Sci. 2026 Oct 28;293(2081):20260507. doi: 10.1098/rspb.2026.0507. PMID- 42947415 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1471-2954 (Electronic) IS - 0962-8452 (Linking) VI - 293 IP - 2081 DP - 2026 Oct 28 TI - The bottom-up effects of aridity and the top-down effects of grazing shape biodiversity across trophic levels in the Inner Mongolian grassland. LID - 20260561 [pii] LID - 10.1098/rspb.2026.0561 [doi] AB - Aridity poses a severe threat to global biodiversity, and grazing further exacerbates ecological stress in drylands. Plants and soil organisms regulate nutrient cycling and ecosystem stability through trophic cascade effects. It remains unclear whether aridity and grazing have consistent effects across trophic levels. We selected 65 paired grazing and ungrazing sites along a natural aridity gradient in the Inner Mongolian grassland in which we explored the diversity of plants, soil bacteria, fungi, nematodes and the impacts of aridity and grazing on trophic cascade effects. Increasing aridity significantly decreased the species richness of plants, pathotrophic fungi, saprotrophic fungi, herbivorous and bacterivorous nematodes. Grazing significantly increased bacterial richness and reduced the richness of herbivorous and omnivorous-predatory nematodes. Overall, aridity exerted a stronger impact on biodiversity compared with grazing. Aridity induced bottom-up effects by initially reducing plant richness, with cascading effects on soil fungal richness and higher trophic communities. Grazing reduced omnivorous-predatory nematode richness, which decreased the richness of lower trophic level organisms through top-down effects. Our research highlights different multi-trophic cascades of aridity and grazing in grasslands, emphasizing the necessity for deeper investigation into biodiversity responses across trophic levels under global change and land use. CI - (c) 2026 The Authors. FAU - Gong, Xiaoqian AU - Gong X AD - School of Ecology and Environment, Inner Mongolia University , 010021, Hohhot, Inner Mongolia, People's Republic of China. FAU - Su, Nier AU - Su N AD - School of Ecology and Environment, Inner Mongolia University , 010021, Hohhot, Inner Mongolia, People's Republic of China. FAU - Wen, Jia AU - Wen J AD - School of Ecology and Environment, Inner Mongolia University , 010021, Hohhot, Inner Mongolia, People's Republic of China. FAU - Ma, Le AU - Ma L AD - School of Ecology and Environment, Inner Mongolia University , 010021, Hohhot, Inner Mongolia, People's Republic of China. FAU - Zhu, Biao AU - Zhu B AD - School of Ecology and Environment, Inner Mongolia University , 010021, Hohhot, Inner Mongolia, People's Republic of China. AD - College of Urban and Environmental Sciences, Peking University , 100871, Beijing, People's Republic of China. FAU - Zhang, Qing AU - Zhang Q AUID- ORCID: 0000-0002-3489-1417 AD - Ministry of Education Key Laboratory of Ecology and Resource Use of the Mongolian Plateau and Inner Mongolia Key Laboratory of Grassland Ecology, School of Ecology and Environment, Inner Mongolia University , 010021, Hohhot, Inner Mongolia, People's Republic of China. LA - eng GR - KCX2024013-1/Major Science and Technology Projects of Inner Mongolia Autonomous Region/ GR - 2019ZD008/Inner Mongolia Autonomous Region Major Special Project/ GR - 2023ZD24/Inner Mongolia Autonomous Region Natural Science Foundation Key Project/ GR - NMGIRT2409/Inner Mongolia Autonomous Region Education Department Project/ PT - Journal Article PL - England TA - Proc Biol Sci JT - Proceedings. Biological sciences JID - 101245157 SB - IM MH - *Grassland MH - *Biodiversity MH - Animals MH - China MH - *Herbivory MH - Soil Microbiology MH - *Food Chain MH - Nematoda MH - *Desert Climate MH - *Droughts OTO - NOTNLM OT - aridity OT - diversity OT - grassland OT - grazing OT - soil organism OT - trophic cascade EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 01:43 PHST- 2026/03/04 00:00 [received] PHST- 2026/08/11 00:00 [revised] PHST- 2026/08/11 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:43 [entrez] AID - 483913 [pii] AID - 10.1098/rspb.2026.0561 [doi] PST - ppublish SO - Proc Biol Sci. 2026 Oct 28;293(2081):20260561. doi: 10.1098/rspb.2026.0561. PMID- 42947411 OWN - NLM STAT- Publisher LR - 20261028 IS - 1097-4598 (Electronic) IS - 0148-639X (Linking) DP - 2026 Oct 27 TI - Time-to-Event Analysis of Clinical Milestones in the ALS Natural History Consortium Dataset. LID - 10.1002/mus.70440 [doi] AB - INTRODUCTION/AIMS: Study of clinical milestones, particularly intermediate events like loss of ambulation, in large amyotrophic lateral sclerosis (ALS) cohorts is limited. Here, we leverage the ALS Natural History Consortium dataset to evaluate the time-to-event for several outcomes and prognostic factors that predict risk. METHODS: Longitudinal data from 1950 participants were used in time-to-event analyses based on time from diagnosis. Outcomes were gastrostomy, loss of ambulation, loss of useful speech, non-invasive ventilation (NIV) usage, and death equivalent (death or mechanical ventilation). Median times-to-event were calculated along with Kaplan-Meier curves. Cox proportional hazards models for each outcome estimated (through adjusted hazard ratios [HR]) the impact of several prognostic factors, including sex, region of onset, age at diagnosis, and the initial rate of change (delta-FRS) in ALS Functional Rating Scale-Revised (ALSFRS-R) scores. RESULTS: Estimated median times-to-event for clinical outcomes ranged from 1.44 to 6.21 years, with a median time to death equivalent of 2.12 years. A higher delta-FRS was the greatest risk factor for all outcomes (HRs between 2.01 and 3.05). Limb onset decreased risk of loss of speech, NIV use, gastrostomy, and death equivalent (HRs 0.41-0.78). Older age at diagnosis increased risk of gastrostomy, NIV use, and death equivalent (HRs 1.08-1.27). DISCUSSION: These findings further inform expectations of time to various outcomes, especially rarely investigated intermediate events, and their risk factors. The importance of progression rate supports other recent findings, highlighting delta-FRS as a key prognostic factor with potential utility in clinical trial designs. CI - (c) 2026 The Author(s). Muscle & Nerve published by Wiley Periodicals LLC. FAU - Duffy, Kelly AU - Duffy K AUID- ORCID: 0000-0001-8476-2077 AD - Masonic Institute for the Developing Brain, University of Minnesota, Minneapolis, Minnesota, USA. FAU - Gwathmey, Kelly AU - Gwathmey K AUID- ORCID: 0000-0002-7589-8826 AD - Department of Neurology, Virginia Commonwealth University, Richmond, Virginia, USA. FAU - Schneck, David AU - Schneck D AD - Masonic Institute for the Developing Brain, University of Minnesota, Minneapolis, Minnesota, USA. FAU - Arguedas, Andres AU - Arguedas A AUID- ORCID: 0000-0001-6299-052X AD - Division of Biostatistics, School of Public Health, University of Minnesota, Minneapolis, Minnesota, USA. FAU - Xenopoulos-Oddsson, Annette AU - Xenopoulos-Oddsson A AUID- ORCID: 0000-0003-0140-4229 AD - Masonic Institute for the Developing Brain, University of Minnesota, Minneapolis, Minnesota, USA. FAU - Heiman-Patterson, Terry AU - Heiman-Patterson T AUID- ORCID: 0000-0002-9910-1041 AD - Department of Neurology, Temple University, Philadelphia, Pennsylvania, USA. FAU - Ajroud-Driss, Senda AU - Ajroud-Driss S AD - Department of Neurology, Northwestern University Feinberg School of Medicine, Chicago, Illinois, USA. FAU - Arcila-Londono, Ximena AU - Arcila-Londono X AUID- ORCID: 0000-0002-2385-4231 AD - Neurology and Neuromuscular Medicine, Henry Ford Health, Detroit, Michigan, USA. FAU - Wymer, James AU - Wymer J AUID- ORCID: 0000-0003-0809-2977 AD - Department of Neurology, University of Florida, Gainesville, Florida, USA. FAU - Olney, Nicholas AU - Olney N AD - Neurology and Neuromuscular Medicine, Providence Neuroscience Institute, Portland, Oregon, USA. FAU - Hayat, Ghazala AU - Hayat G AD - Department of Neurology, Saint Louis University, St. Louis, Missouri, USA. FAU - Lunetta, Christian AU - Lunetta C AUID- ORCID: 0000-0002-4788-1875 AD - Department of Neurorehabilitation of Milan Institute, Istituti Clinici Scientifici Maugeri IRCCS, Milan, Italy. FAU - Cerri, Federica AU - Cerri F AUID- ORCID: 0009-0003-1619-2019 AD - Department of Neurology, Centro Clinico NeMO, Milan, Italy. FAU - Al-Lahham, Tawfiq AU - Al-Lahham T AUID- ORCID: 0009-0001-5915-2215 AD - Department of Neurology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA. FAU - Sherman, Alex AU - Sherman A AUID- ORCID: 0000-0003-2097-5289 AD - Center for Innovation and Bioinformatics, Massachusetts General Hospital, Boston, Massachusetts, USA. FAU - Ghasemi, Mehdi AU - Ghasemi M AUID- ORCID: 0000-0002-1384-9826 AD - Lahey Hospital and Medical Center, UMass Chan School of Medicine, Burlington, Massachusetts, USA. FAU - Cui, Erjia AU - Cui E AUID- ORCID: 0000-0003-3576-2892 AD - Masonic Institute for the Developing Brain, University of Minnesota, Minneapolis, Minnesota, USA. AD - Division of Biostatistics, School of Public Health, University of Minnesota, Minneapolis, Minnesota, USA. FAU - Fiecas, Mark AU - Fiecas M AUID- ORCID: 0000-0002-6472-9410 AD - Division of Biostatistics, School of Public Health, University of Minnesota, Minneapolis, Minnesota, USA. FAU - Walk, David AU - Walk D AUID- ORCID: 0000-0001-7941-1993 AD - Department of Neurology, University of Minnesota, Minneapolis, Minnesota, USA. LA - eng GR - FD-R01-007630/FD/FDA HHS/United States GR - Department of Neurology, University of Minnesota/ PT - Journal Article DEP - 20261027 PL - United States TA - Muscle Nerve JT - Muscle & nerve JID - 7803146 SB - IM OTO - NOTNLM OT - Cox proportional hazards model OT - amyotrophic lateral sclerosis OT - natural history study OT - prognostic factors OT - time-to-event EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 01:43 PHST- 2026/10/08 00:00 [revised] PHST- 2026/02/18 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:43 [entrez] AID - 10.1002/mus.70440 [doi] PST - aheadofprint SO - Muscle Nerve. 2026 Oct 27. doi: 10.1002/mus.70440. PMID- 42947409 OWN - NLM STAT- Publisher LR - 20261028 IS - 1365-2516 (Electronic) IS - 1351-8216 (Linking) DP - 2026 Oct 27 TI - Arthroplasty After Hemophilia Gene Therapy: Perioperative Implications and Clinical Experience. LID - 10.1111/hae.70429 [doi] AB - INTRODUCTION: Gene therapy can provide sustained endogenous factor VIII (FVIII) or factor IX (FIX) expression, creating a perioperative phenotype that differs from both conventionally treated severe hemophilia and routine arthroplasty patients. Evidence for major joint replacement after gene therapy remains limited. AIM: To synthesize current clinical evidence on arthroplasty after hemophilia gene therapy and define practical implications for hemostatic management, venous thromboembolism (VTE) prevention, rehabilitation, and multidisciplinary planning. METHODS: PubMed/MEDLINE and reference lists were searched through September 2026 for direct arthroplasty reports, invasive-procedure studies after gene therapy, and current guidance relevant to surgery and thromboprophylaxis. RESULTS: Published evidence includes dedicated total knee and total hip arthroplasty reports, trial-level arthroplasty observations, and broader invasive-procedure datasets after valoctocogene roxaparvovec, etranacogene dezaparvovec, and fidanacogene elaparvovec. These data show that gene therapy may substantially reduce exogenous factor requirements, but they do not establish a validated FVIII or FIX threshold for arthroplasty. Current World Federation of Hemophilia guidance recommends achieving procedure-appropriate factor levels, with supplemental clotting factor concentrate when necessary. In patients whose factor activity has shifted toward mild-deficiency or nonhemophilic levels, standard arthroplasty VTE-prevention principles should not be withheld solely because of the historical diagnosis of hemophilia. CONCLUSION: Arthroplasty after hemophilia gene therapy should be managed as phenotype-based major surgery: procedure-appropriate hemostasis, immediate rescue-factor access, serial postoperative monitoring, and appropriately timed VTE prophylaxis remain essential. CI - (c) 2026 John Wiley & Sons Ltd. FAU - Vu, Dinh Nhat AU - Vu DN AD - Institute of Biomedicine and Pharmacy, Vietnam Military Medical University, Hanoi, Vietnam. AD - Department of Orthopaedic and Trauma, Vietnam Military Medical University, Hanoi, Vietnam. FAU - Nguyen, Thanh Tien AU - Nguyen TT AD - Department of Joint Surgery, 108 Military Central Hospital, Hanoi, Vietnam. FAU - Pham, Thang Ngoc AU - Pham TN AD - Department of Orthopaedic and Trauma, Vietnam Military Medical University, Hanoi, Vietnam. AD - Department of Joint Surgery, 103 Military Hospital, Hanoi, Vietnam. FAU - Vu, Dung Anh AU - Vu DA AUID- ORCID: 0009-0007-4427-3739 AD - Department of Joint Surgery, 103 Military Hospital, Hanoi, Vietnam. AD - Graduate School of Medical Science, Kanazawa Medical University, Ishikawa, Japan. LA - eng GR - RY053565/Rotary Yoneyama Memorial Foundation/ PT - Journal Article PT - Review DEP - 20261027 PL - England TA - Haemophilia JT - Haemophilia : the official journal of the World Federation of Hemophilia JID - 9442916 SB - IM OTO - NOTNLM OT - arthroplasty OT - clotting factor replacement OT - gene therapy OT - hemophilia OT - thromboprophylaxis EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 01:43 PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:43 [entrez] AID - 10.1111/hae.70429 [doi] PST - aheadofprint SO - Haemophilia. 2026 Oct 27. doi: 10.1111/hae.70429. PMID- 42947406 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2291-2797 (Electronic) IS - 2291-2789 (Print) IS - 2291-2789 (Linking) VI - 2026 IP - 1 DP - 2026 TI - Predicting Liver Stiffness Approximately 12 Months Ahead Using Serial Transient Elastography. LID - e1261185 [pii] LID - 10.1155/cjgh/1261185 [doi] LID - 1261185 AB - BACKGROUND: Liver stiffness measurement (LSM) is widely used to assess chronic liver disease, but a single measurement does not capture longitudinal change. We evaluated whether serial LSMs and routine clinical indicators could predict continuous LSM approximately 12 months later. METHODS: This retrospective longitudinal study included patients with at least two historical LSMs and a follow-up LSM 270-450 days after the index date. The LSM closest to 365 days was the outcome, and each patient contributed the earliest eligible task. Patients were separated chronologically into a train set (index date through 2022), test set (2023), and validation set (2024). Nested expanding temporal cross-validation selected the model, which was then fixed and compared with longitudinal benchmarks. Patient-level bootstrapping provided 95% confidence intervals (CIs). RESULTS: The analysis included 715 patients (train, 434; test, 178; validation, 103). Random forest was selected. In the test set, its mean absolute error (MAE) was 3.622 kPa (95% CI, 2.828-4.769), R(2) was 0.456 (0.240-0.550), and calibration slope was 1.043 (0.612-1.475). In the validation set, MAE was 2.989 kPa (2.149-4.149), R(2) was 0.432 (-0.141-0.575), and calibration slope was 0.951 (0.513-1.598). In the test set, the model reduced MAE by 8.443 kPa (4.833-12.605) versus linear-trend extrapolation; however, the last observed LSM performed better, with a paired last-observed-LSM-minus-model MAE difference of -0.658 kPa (-1.168 to -0.187). Historical LSM summaries were the dominant features. CONCLUSIONS: The locked model showed reproducible out-of-time performance and near-ideal calibration point estimates but did not outperform the last observed LSM. Independent multicenter validation is required before clinical translation. TRIAL REGISTRATION: ClinicalTrials.gov identifier: NCT06123858. CI - Copyright (c) 2026 Ming Lei et al. Canadian Journal of Gastroenterology and Hepatology published by John Wiley & Sons Ltd. FAU - Lei, Ming AU - Lei M AUID- ORCID: 0009-0004-6130-7759 AD - Department of Hemodialysis, Dalian Public Health Clinical Center, No. 269, Gui Bai Road, Lu Gang, Ganjingzi District, Dalian, Liaoning, China. FAU - Zou, Shoukang AU - Zou S AUID- ORCID: 0009-0002-7667-9016 AD - Unit 4-Department of Geriatric Medicine, The Fourth People's Hospital of Chengdu, No.8 Huli-West First-Alley, Jinniu District, Chengdu, Sichuan, China. FAU - Liu, Qing AU - Liu Q AUID- ORCID: 0009-0003-5229-1903 AD - Department of Laboratory Medicine, Liaoning Electric Power Central Hospital., No. 2, Wanghu Road, Heping District, Shenyang, Liaoning, China. FAU - Li, Yangling AU - Li Y AUID- ORCID: 0009-0007-5691-8071 AD - Department of Gastroenterology, West China Hospital of Sichuan University, No. 37, Guoxue Lane, Wuhou District, Chengdu, Sichuan, China, wchscu.cn. FAU - Han, Jing AU - Han J AUID- ORCID: 0009-0000-5028-2489 AD - Department of Hemodialysis, Dalian Public Health Clinical Center, No. 269, Gui Bai Road, Lu Gang, Ganjingzi District, Dalian, Liaoning, China. FAU - Zhang, Yungang AU - Zhang Y AUID- ORCID: 0009-0000-0106-416X AD - Department of Hemodialysis, Dalian Public Health Clinical Center, No. 269, Gui Bai Road, Lu Gang, Ganjingzi District, Dalian, Liaoning, China. FAU - Pu, Chunwen AU - Pu C AUID- ORCID: 0009-0007-9150-0543 AD - Biobank for Biological Samples and Data, Dalian Public Health Clinical Center, No. 269, Gui Bai Road, Lu Gang, Ganjingzi District, Dalian, Liaoning, China. LA - eng SI - ClinicalTrials.gov/NCT06123858 GR - 2024JJ13PT062/Dalian Municipal Bureau of Science and Technology/ GR - 2023-243-2/Dalian Municipal Bureau of Science and Technology/ PT - Journal Article PL - Egypt TA - Can J Gastroenterol Hepatol JT - Canadian journal of gastroenterology & hepatology JID - 101623613 SB - IM MH - Humans MH - *Elasticity Imaging Techniques/methods MH - Retrospective Studies MH - Female MH - Longitudinal Studies MH - Male MH - Middle Aged MH - *Liver/diagnostic imaging/pathology MH - Aged MH - Predictive Value of Tests MH - Time Factors MH - *Liver Cirrhosis/diagnostic imaging MH - Random Forest MH - *Liver Diseases/diagnostic imaging MH - Reproducibility of Results PMC - PMC13714349 OTO - NOTNLM OT - liver stiffness measurement OT - longitudinal prediction OT - machine learning OT - temporal validation OT - transient elastography COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/27 CRDT- 2026/10/28 01:43 PHST- 2026/09/03 00:00 [revised] PHST- 2026/03/25 00:00 [received] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:43 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e1261185 [pii] AID - CJGH1261185 [pii] AID - 10.1155/cjgh/1261185 [doi] PST - ppublish SO - Can J Gastroenterol Hepatol. 2026;2026(1):1261185. doi: 10.1155/cjgh/1261185. PMID- 42947399 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1940-3372 (Electronic) IS - 1940-3372 (Linking) VI - 19 IP - 4 DP - 2026 Dec TI - Reaction norm modeling of high-dimensional genomic and environmental data improves prediction accuracy in winter wheat. LID - e70332 [pii] LID - 10.1002/tpg2.70332 [doi] AB - Genomic prediction models that account for genotype-by-environment (G x E) have the potential to accelerate the rate of genetic gain for yield and agronomic performance, yet relatively few studies have applied G x E prediction in public soft red winter wheat (Triticum aestivum) breeding programs. In this study, we extended a reaction norm-based genomic prediction framework by integrating weather-based environmental covariates to more effectively capture G x E interactions. Key agronomic traits, including seed yield, plant height, test weight, and heading date, were evaluated across 25 environments (location-year) using over 3200 breeding lines from the North Carolina State University small grains breeding program. Multiple genomic prediction models were compared using several cross-validation (CV) schemes representing common breeding scenarios. Across traits, the reaction norm M5 model, which incorporates both G x E and genotype-by-environmental covariate interactions (G x O), achieved the highest prediction accuracy (PA) in CV2 (predicting incomplete field trials) and CV1 for yield and test weight (predicting new lines). The highest PA was observed for test weight under CV2 (0.55) and for yield under CV1 (0.41). Under CV0 (predicting new environments), the M3 model incorporating G x E produced the highest PA across traits, with the greatest accuracy for plant height (0.45), although differences among M2, M3, and M4 were small. Prediction under CV00 (predicting new lines in new environments) remained more challenging, with PA values 0.10-0.20 across traits. Overall, our results demonstrate that integrating environmental covariates into genomic prediction models can improve predictive performance across diverse wheat-growing environments in North Carolina, supporting their utility for applied breeding efforts. CI - (c) 2026 The Author(s). The Plant Genome published by Wiley Periodicals LLC on behalf of Crop Science Society of America. FAU - Acharya, Shailesh Raj AU - Acharya SR AUID- ORCID: 0009-0007-5692-9248 AD - Department of Crop and Soil Sciences, North Carolina State University, Raleigh, North Carolina, USA. FAU - Garcia-Abadillo, Julian AU - Garcia-Abadillo J AUID- ORCID: 0000-0002-4672-8908 AD - Department of Agronomy, University of Florida, Gainesville, Florida, USA. FAU - Lyerly, Jeanette AU - Lyerly J AUID- ORCID: 0000-0003-3853-9581 AD - Department of Crop and Soil Sciences, North Carolina State University, Raleigh, North Carolina, USA. FAU - Brown-Guedira, Gina AU - Brown-Guedira G AUID- ORCID: 0000-0002-1958-2827 AD - USDA-ARS SEA, Plant Science Research, Raleigh, North Carolina, USA. FAU - Jarquin, Diego AU - Jarquin D AUID- ORCID: 0000-0002-5098-2060 AD - Department of Agronomy, University of Florida, Gainesville, Florida, USA. FAU - Bandillo, Nonoy AU - Bandillo N AUID- ORCID: 0000-0002-5941-9047 AD - Department of Crop and Soil Sciences, North Carolina State University, Raleigh, North Carolina, USA. LA - eng GR - 04027/NCSU Bandillo Hatch Funding/ GR - 59-0206-4-046/US Wheat and Barley Scab Initiative/ GR - 2022-68013-36439/USDA Agriculture and Food Research Initiative/ GR - North Carolina Small Grains Growers Association/ PT - Journal Article PL - United States TA - Plant Genome JT - The plant genome JID - 101273919 SB - IM MH - *Triticum/genetics/growth & development MH - *Gene-Environment Interaction MH - *Models, Genetic MH - Genotype MH - *Genomics/methods MH - Plant Breeding MH - Phenotype MH - Environment MH - *Genome, Plant MH - Prediction Algorithms PMC - PMC13714343 OAB - This study explores how adding environmental information to genomic prediction models can improve prediction accuracy in a public winter wheat breeding program. Using about 3200 breeding lines evaluated in 25 environments across diverse conditions in North Carolina, we evaluated statistical models that capture how different wheat lines respond to changing environments. By incorporating weather data, we improved the ability to predict performance across locations and years. These findings provide practical insights for refining selection strategies and accelerating genetic gain in wheat breeding. OABL- eng COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/27 CRDT- 2026/10/28 01:34 PHST- 2026/04/15 00:00 [received] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:34 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e70332 [pii] AID - TPG270332 [pii] AID - 10.1002/tpg2.70332 [doi] PST - ppublish SO - Plant Genome. 2026 Dec;19(4):e70332. doi: 10.1002/tpg2.70332. PMID- 42947391 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1744-957X (Electronic) IS - 1744-9561 (Linking) VI - 22 IP - 10 DP - 2026 Oct 28 TI - A novel supergene regulates alternative dispersal morphs without variation in social organization in the ant Myrmecina nipponica. LID - 20260475 [pii] LID - 10.1098/rsbl.2026.0475 [doi] AB - Polymorphisms in complex traits with discrete phenotypes are often underpinned by non-recombining complexes of co-adaptive genes, known as supergenes. In disparate lineages of ants, it is increasingly evident that supergenes are important in regulating intra-specific variation in social organization (number of reproductive queens in a colony). Variation in social organization in ants is often associated with variation in dispersal, with multiple-queen (polygynous) colony types exhibiting more limited dispersal than single-queen (monogynous) colony types. It has been suggested that this association may give rise to antagonistic selection, which in turn may help explain the multiple independent evolutions of social supergenes in ants. In this study, we provide evidence of an exception to this pattern in the form of a novel supergene in the ant Myrmecina nipponica. This species exhibits a dispersal polymorphism with winged and wingless queens but without variation in social structure, as all colonies have a single, reproductive queen. We show that the dispersal polymorphism in this species is underpinned by an approximately 6.8 Mb supergene novel to ants. Interestingly, this supergene is structurally and functionally different from that recently reported in another member of this genus, illustrating how patterns of supergene evolution can show marked intrageneric variation. CI - (c) 2026 The Authors. FAU - Liprado, Ermae AU - Liprado E AUID- ORCID: 0009-0003-3425-7760 AD - Department of Biological Sciences, Tokyo Metropolitan University , Hachioji, Japan. FAU - Planas-Sitja, Isaac AU - Planas-Sitja I AD - Department of Biological Sciences, Tokyo Metropolitan University , Hachioji, Japan. AD - Center for Interdisciplinary Theoretical and Mathematical Sciences (iTHEMS), RIKEN Wako Campus , 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. FAU - Okamura, Yu AU - Okamura Y AD - Department of Biological Sciences, Tokyo Metropolitan University , Hachioji, Japan. FAU - Cronin, Adam L AU - Cronin AL AUID- ORCID: 0000-0003-2075-1138 AD - Department of Biological Sciences, Tokyo Metropolitan University , Hachioji, Japan. LA - eng GR - Tokyo Metropolitan University/ PT - Journal Article PL - England TA - Biol Lett JT - Biology letters JID - 101247722 SB - IM MH - Animals MH - *Ants/genetics/physiology MH - *Social Behavior MH - Female MH - *Genes, Insect MH - Polymorphism, Genetic MH - *Animal Distribution OTO - NOTNLM OT - dispersal OT - formicidae OT - polymorphism OT - spatially variable selection EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 01:33 PHST- 2026/03/17 00:00 [received] PHST- 2026/09/02 00:00 [accepted] PHST- 2026/08/09 00:00 [revised] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:33 [entrez] AID - 483907 [pii] AID - 10.1098/rsbl.2026.0475 [doi] PST - ppublish SO - Biol Lett. 2026 Oct 28;22(10):20260475. doi: 10.1098/rsbl.2026.0475. PMID- 42947390 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1744-957X (Electronic) IS - 1744-9561 (Linking) VI - 22 IP - 10 DP - 2026 Oct 28 TI - Do herbivorous fish amplify the competitive dominance of floating-leaved plants over submerged plants? LID - 20260343 [pii] LID - 10.1098/rsbl.2026.0343 [doi] AB - Growing interest in the relationship between herbivorous fish and aquatic plants has revealed multiple pathways by which fish can influence aquatic plant biomass and coverage. However, the impact of herbivorous fish on species competition and community assembly in aquatic plants remains poorly understood. Here, based on a mesocosm experiment with four aquatic plant species (two floating-leaved and two submerged) and one herbivorous fish, we quantified competition coefficients among plant species and fish effects using a modified Ricker model. Floating-leaved plants exhibited stronger intraspecific than interspecific competition, indicating competitive superiority over submerged species. Crucially, selective grazing by herbivorous fish on submerged plants indirectly facilitated the dominance of floating-leaved species, exacerbating competitive asymmetry. These results demonstrate that herbivory indirectly affects aquatic plant community structure, not simply by removing biomass but also via higher-order interactions. Our research extends herbivore-plant interaction theory to freshwater ecosystems and offers guidance for aquatic plant restoration and biological management. CI - (c) 2026 The Authors. FAU - Xia, Manli AU - Xia M AUID- ORCID: 0000-0002-6176-3875 AD - Research Center of Aquatic Plant and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences , Wuhan, Hubei, People's Republic of China. FAU - Ye, Zhong-Ming AU - Ye ZM AUID- ORCID: 0000-0002-8290-0891 AD - Research Center of Aquatic Plant and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences , Wuhan, Hubei, People's Republic of China. FAU - Quan, Guoqing AU - Quan G AD - Research Center of Aquatic Plant and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences , Wuhan, Hubei, People's Republic of China. AD - College of Horticulture and Gardening, Yangtze University , Jingzhou, Hubei, People's Republic of China. FAU - Liu, Wei AU - Liu W AD - Research Center of Aquatic Plant and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences , Wuhan, Hubei, People's Republic of China. AD - College of Horticulture and Gardening, Yangtze University , Jingzhou, Hubei, People's Republic of China. FAU - Ma, Hui AU - Ma H AD - Research Center of Aquatic Plant and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences , Wuhan, Hubei, People's Republic of China. AD - College of Resources and Environment, Huazhong Agricultural University , Wuhan, People's Republic of China. FAU - Li, Wei AU - Li W AD - Yani Wetland Ecosystem Positioning Observation and Research Station, Xizang University , Lhasa, Tibet, People's Republic of China. AD - Key Laboratory of Biodiversity Conservation and Ecological Restoration , Shannan, People's Republic of China. AD - Nyingchi National Botanical Garden , Nyingchi, People's Republic of China. FAU - Cao, Yu AU - Cao Y AD - Research Center of Aquatic Plant and Watershed Ecology, Wuhan Botanical Garden, Chinese Academy of Sciences , Wuhan, Hubei, People's Republic of China. LA - eng GR - 32370388/National Natural Science Foundation of China/ PT - Journal Article PL - England TA - Biol Lett JT - Biology letters JID - 101247722 SB - IM MH - Animals MH - *Herbivory MH - *Fishes/physiology MH - Biomass MH - Ecosystem OTO - NOTNLM OT - asymmetric competition OT - biotic interactions OT - community succession OT - selective herbivory EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 01:33 PHST- 2026/05/28 00:00 [received] PHST- 2026/08/26 00:00 [accepted] PHST- 2026/08/17 00:00 [revised] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:33 [entrez] AID - 483906 [pii] AID - 10.1098/rsbl.2026.0343 [doi] PST - ppublish SO - Biol Lett. 2026 Oct 28;22(10):20260343. doi: 10.1098/rsbl.2026.0343. PMID- 42947386 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1464-5157 (Electronic) IS - 0265-6736 (Linking) VI - 43 IP - 1 DP - 2026 Dec TI - Focused ultrasound versus LEEP for high-grade squamous intraepithelial lesions: a retrospective cohort study of long-term effectiveness and obstetric outcomes. PG - 2749296 LID - 10.1080/02656736.2026.2749296 [doi] AB - INTRODUCTION: To compare long-term effectiveness and obstetric outcomes of focused ultrasound (FU) ablation versus loop electrosurgical excision procedure (LEEP) for high-grade squamous intraepithelial lesions (HSIL) in reproductive-aged women. MATERIALS AND METHODS: This retrospective cohort included women treated with FU (n = 29) or LEEP (n = 32) for HSIL who subsequently delivered (2017-2023). A control group (n = 61) comprised healthy women with negative preconception HR-HPV/cytology. Outcomes were cumulative HR-HPV clearance, cervical length, pregnancy outcomes. RESULTS: Both FU and LEEP demonstrated comparable long-term effectiveness: cumulative HR-HPV clearance rates did not differ significantly at any time point (p > 0.05). Post-intervention cervical length was significantly shorter in the LEEP group (24.79 +/- 3.98 mm) than in both the FU (27.63 +/- 2.80 mm) and control (28.26 +/- 3.92 mm) groups (p = 0.003). Cervical insufficiency was more frequent in the LEEP group (15.63%) than in the FU (3.45%) and control (1.64%) groups (p = 0.019). The LEEP group also had higher rates of preterm birth (18.75% vs 3.45% in FU and 4.92% in controls; p = 0.039) and lower rates of vaginal delivery (15.63% vs 44.83% in FU; p = 0.025). CONCLUSION: In this selected cohort of women with successful live births, FU may have comparable long-term effectiveness to LEEP for HSIL while potentially preserving cervical length and reducing adverse obstetric risks. These preliminary findings suggest FU may be a fertility-sparing option for appropriately selected HSIL women who are planning pregnancy. FAU - Zhang, Miao AU - Zhang M AD - Department of Gynecology and Obstetrics, Affiliated Hospital of North Sichuan Medical College, Nanchong, China. FAU - Long, Fei AU - Long F AD - North Sichuan Medical College, Nanchong, China. FAU - Liu, Yujuan AU - Liu Y AD - Department of Gynecology and Obstetrics, Affiliated Hospital of North Sichuan Medical College, Nanchong, China. FAU - Jing, Qiao AU - Jing Q AD - Department of Gynecology and Obstetrics, Affiliated Hospital of North Sichuan Medical College, Nanchong, China. FAU - Ren, Jiaojiao AU - Ren J AD - Department of Gynecology and Obstetrics, Affiliated Hospital of North Sichuan Medical College, Nanchong, China. FAU - Chen, Liming AU - Chen L AD - Department of Gynecology and Obstetrics, Affiliated Hospital of North Sichuan Medical College, Nanchong, China. FAU - Li, Chenzhi AU - Li C AD - State Key Laboratory of Ultrasound in Medicine and Engineering College of Biomedical Engineering, Chongqing Medical University, Chongqing, China. FAU - Shi, Qiuling AU - Shi Q AD - State Key Laboratory of Ultrasound in Medicine and Engineering College of Biomedical Engineering, Chongqing Medical University, Chongqing, China. FAU - Zhou, Honggui AU - Zhou H AD - Department of Gynecology and Obstetrics, Affiliated Hospital of North Sichuan Medical College, Nanchong, China. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Int J Hyperthermia JT - International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group JID - 8508395 SB - IM MH - Humans MH - Female MH - Retrospective Studies MH - *Electrosurgery/methods MH - Pregnancy MH - Adult MH - *Squamous Intraepithelial Lesions/surgery MH - Pregnancy Outcome MH - *High-Intensity Focused Ultrasound Ablation/methods OTO - NOTNLM OT - Cervical length OT - focused ultrasound OT - high-grade squamous intraepithelial lesion OT - loop electrosurgical excision procedure OT - pregnancy outcomes EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 01:33 PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:33 [entrez] AID - 10.1080/02656736.2026.2749296 [doi] PST - ppublish SO - Int J Hyperthermia. 2026 Dec;43(1):2749296. doi: 10.1080/02656736.2026.2749296. Epub 2026 Oct 27. PMID- 42947385 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1439-0531 (Electronic) IS - 0936-6768 (Print) IS - 0936-6768 (Linking) VI - 61 IP - 11 DP - 2026 Nov TI - Impact of Reactive Temperament on Bull Fertility in Natural Service. LID - e70324 [pii] LID - 10.1111/rda.70324 [doi] AB - The study aimed to evaluate the influence of temperament on semen quality and fertility in Nelore bulls. Two groups were selected based on ethological analysis, consisting of animals with either calm temperament (CA, n = 8) or reactive temperament (RE, n = 8). All bulls underwent two semen collections for sperm evaluation (pre- and post-thawing), as well as serial blood collections for testosterone and cortisol measurements. Ejaculates recovered from the three most reactive and the three least reactive bulls were pooled to form two semen batches, which were subsequently cryopreserved and used for fixed-time artificial insemination (FTAI) to evaluate the fertility of semen from bulls with the two different temperament profiles. Additionally, each bull was housed in individual paddocks with cyclic cows (average of 20 cows per bull) for 90 days for a natural service capacity test. No differences were observed in sperm kinetic parameters, morphology and plasma membrane integrity for fresh or post-thaw semen when comparing the two experimental groups. Likewise, no effect of reactivity was observed on the fertility of calm and reactive bull semen in FTAI (p > 0.05). CA bulls showed lower average cortisol concentrations compared to the RE group during the second experimental routine, but there were no differences in testosterone values. CA bulls achieved higher conception rates in natural service (84.56%) compared to reactive animals (76.41%; p < 0.0001). In conclusion, no significant effects of temperament on fresh or post-thaw semen quality parameters were detected in Nelore bulls under the conditions of this study. However, bulls with a calmer temperament exhibited higher reproductive performance in natural service programs, supporting the hypothesis that behavioural mating efficiency rather than intrinsic gametic failure influences pasture breeding success. CI - (c) 2026 The Author(s). Reproduction in Domestic Animals published by Wiley-VCH GmbH. FAU - de Almeida Martin, Larissa AU - de Almeida Martin L AD - Santo Amaro University, UNISA, Sao Paulo, Brazil. FAU - da Silva, Mariana Karla Francolino AU - da Silva MKF AUID- ORCID: 0009-0008-5494-6025 AD - Department of Animal Reproduction, University of Sao Paulo (USP), Sao Paulo, Brazil. FAU - Sant'Ana Borges, Marcelo AU - Sant'Ana Borges M AUID- ORCID: 0000-0002-8842-5710 AD - Institute of Animal Science, Sertaozinho, Brazil. FAU - Fernandes, Luana Gomes AU - Fernandes LG AD - Sao Paulo State University, Jaboticabal, Brazil. FAU - Landgraf, Richardt Gama AU - Landgraf RG AUID- ORCID: 0000-0001-7774-7880 AD - Department of Pharmaceutical Sciences, Federal University of Sao Paulo, UNIFESP, Diadema, Brazil. FAU - Monteiro, Fabio Morato AU - Monteiro FM AUID- ORCID: 0000-0003-4344-4667 AD - Institute of Animal Science, Sertaozinho, Brazil. FAU - Crespilho, Andre Maciel AU - Crespilho AM AD - Santo Amaro University, UNISA, Sao Paulo, Brazil. AD - Central Bela Vista, Botucatu, Sao Paulo, Brazil. LA - eng GR - 2019/06456-2/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/ PT - Journal Article PL - Germany TA - Reprod Domest Anim JT - Reproduction in domestic animals = Zuchthygiene JID - 9015668 RN - 3XMK78S47O (Testosterone) RN - WI4X0X7BPJ (Hydrocortisone) SB - IM MH - Animals MH - Male MH - Cattle/physiology/psychology MH - *Temperament/physiology MH - *Fertility/physiology MH - Testosterone/blood MH - Semen Analysis/veterinary MH - Insemination, Artificial/veterinary MH - Hydrocortisone/blood MH - Female MH - Spermatozoa/physiology MH - Semen Preservation/veterinary MH - Cryopreservation/veterinary MH - Pregnancy MH - Sperm Motility PMC - PMC13714335 OTO - NOTNLM OT - bull OT - fertility OT - natural service OT - reactiveness OT - semen quality COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/28 CRDT- 2026/10/28 01:33 PHST- 2026/09/24 00:00 [revised] PHST- 2026/06/19 00:00 [received] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:33 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e70324 [pii] AID - RDA70324 [pii] AID - 10.1111/rda.70324 [doi] PST - ppublish SO - Reprod Domest Anim. 2026 Nov;61(11):e70324. doi: 10.1111/rda.70324. PMID- 42947384 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1940-3372 (Electronic) IS - 1940-3372 (Linking) VI - 19 IP - 4 DP - 2026 Dec TI - Development of conditional cores via supersaturated designs for genetic diversity-based prediction models. LID - e70334 [pii] LID - 10.1002/tpg2.70334 [doi] AB - Genetic diversity is fundamental to sustainable crop improvement. This study uses 1755 soybean [Glycine max (L.) Merr.] recombinant inbred lines from the soybean nested association mapping population, phenotyped for yield across nine environments to optimize the composition of the training set for a given testing set. Using supersaturated designs, we generated conditionally optimized training sets by iteratively selecting candidate genotypes according to a metric that quantifies diversity as the trace of the cross-product of the genomic matrix containing both a candidate training set and the fixed testing set. Two strategies were considered: (i) maximizing and (ii) minimizing genetic distances to the testing set. Our results show that maximizing genetic distance relative to the testing set improves prediction accuracy over random training sets under standard genomic best linear unbiased prediction. However, this advantage disappeared when models incorporated family structure or genotype-by-environment interactions, which consistently yielded superior overall accuracy regardless of training set selection. This result highlights the complexity of considering multi-omics integration for interpretability: determining whether an increase in accuracy comes from a better model specification or from capturing confounding factors. Finally, we also found that maximizing the genetic diversity of the training set with respect to the testing set preserves at least one individual from as many different families as possible. In contrast, minimizing diversity either excludes or overrepresents families. Further studies should consider a completely unbalanced dataset that mimics the real-life scenarios breeders face in their programs. CI - (c) 2026 The Author(s). The Plant Genome published by Wiley Periodicals LLC on behalf of Crop Science Society of America. FAU - Zhou, Xiangming AU - Zhou X AD - Agronomy Department, University of Florida, Gainesville, Florida, USA. FAU - Garcia-Abadillo, Julian AU - Garcia-Abadillo J AUID- ORCID: 0000-0002-4672-8908 AD - Agronomy Department, University of Florida, Gainesville, Florida, USA. FAU - Jarquin, Diego AU - Jarquin D AUID- ORCID: 0000-0002-5098-2060 AD - Agronomy Department, University of Florida, Gainesville, Florida, USA. LA - eng PT - Journal Article PL - United States TA - Plant Genome JT - The plant genome JID - 101273919 SB - IM MH - *Glycine max/genetics MH - *Genetic Variation MH - *Models, Genetic MH - Genotype MH - Prediction Algorithms PMC - PMC13714344 OAB - This study proposes the development of training sets customized for targeted testing sets by maximizing/minimizing the genomic distances of the training set with respect to the testing set. It investigates how training populations affects yield prediction in soybeans. The dataset included 1755 soybean lines from a NAM population phenotyped across nine environments. Diversity of the training set was calculated based on the marker single-nucleotide polymorphisms genomic relationship matrix by combining candidate training sets and the testing set. Maximizing genetic diversity resulted in a greater number of families being represented in the training set, whereas minimizing diversity led to the overrepresentation of certain families and the exclusion of others. While maximizing genetic diversity increased predictive ability in simple models using only genomic markers, no advantage was found in more complex models accounting for genotype-by-environment interactions or family information. These results highlight the complexity of considering multi-omics integration for interpretability. OABL- eng COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/27 CRDT- 2026/10/28 01:33 PHST- 2026/04/27 00:00 [received] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:33 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e70334 [pii] AID - TPG270334 [pii] AID - 10.1002/tpg2.70334 [doi] PST - ppublish SO - Plant Genome. 2026 Dec;19(4):e70334. doi: 10.1002/tpg2.70334. PMID- 42947377 OWN - NLM STAT- Publisher LR - 20261028 IS - 1532-950X (Electronic) IS - 0161-3499 (Linking) DP - 2026 Oct 27 TI - Coronoidectomy for treatment of osteomyelitis of the coronoid process: A novel technique. LID - 10.1111/vsu.70173 [doi] AB - OBJECTIVE: To report a minimally invasive coronoidectomy technique for the treatment of osteomyelitis of the mandibular coronoid process. ANIMAL: 2-year-old Haflinger mare. STUDY DESIGN: Case report. METHODS: A 2-year-old Haflinger mare presented with a 6-week history of a non-healing wound caudodorsal to the left eye over the supraorbital fossa. A moderate amount of purulent, hemorrhagic discharge was present with a mild degree of soft tissue swelling. The horse was reactive to palpation of the region. Radiographic examination did not reveal any osseous abnormalities or foreign material in the wound tract. Computed tomography findings were consistent with chronic osteomyelitis of the left coronoid process of the mandible. A minimally invasive coronoidectomy was performed under general anesthesia to remove the left coronoid process of the mandible. RESULTS: The coronoid process of the mandible was successfully removed and the wound healed. No complications were reported postoperatively. At 1 year postoperatively the horse was being used for its intended purpose, and the owner deemed the outcome successful. CONCLUSION: The surgical technique reported presents a minimally invasive approach to excise the mandibular coronoid process in a single case. CI - (c) 2026 The Author(s). Veterinary Surgery published by Wiley Periodicals LLC on behalf of American College of Veterinary Surgeons. FAU - Friedlund, Alannah M AU - Friedlund AM AUID- ORCID: 0009-0001-0960-5503 AD - Department Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. FAU - Thomas, Keri L AU - Thomas KL AUID- ORCID: 0000-0001-9853-5561 AD - Department Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. LA - eng PT - Case Reports PT - Journal Article DEP - 20261027 PL - United States TA - Vet Surg JT - Veterinary surgery : VS JID - 8113214 SB - IM EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 01:23 PHST- 2026/09/30 00:00 [revised] PHST- 2026/06/06 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:23 [entrez] AID - 10.1111/vsu.70173 [doi] PST - aheadofprint SO - Vet Surg. 2026 Oct 27. doi: 10.1111/vsu.70173. PMID- 42947369 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1742-5662 (Electronic) IS - 1742-5689 (Print) IS - 1742-5662 (Linking) VI - 23 IP - 243 DP - 2026 Oct 7 TI - Gravity-driven flow for intracranial saccular aneurysm filling. LID - 10.1098/rsif.2025.0968 [doi] LID - 20250968 AB - Intracranial aneurysms (IAs) are life-threatening cerebrovascular malformations in which complete and durable sac closure is the primary goal of endovascular treatments, including coiling, stent-assisted coiling and flow-diverter implantation. However, standard catheter-based approaches may fail to fully occlude the sac and are limited by the need for direct access. Liquid embolic agents offer better geometric conformity, but their deployment remains constrained by similar access issues. In this study, we present a gravity-driven approach for distal filling of IAs. Through a combination of analytical modelling, in vitro experiments with idealized aneurysm models, and patient-specific computational fluid dynamics (CFD) simulations, we demonstrate that efficient filling of aneurysm cavities from relatively remote locations may be achieved using a heavier injectable fluid, while properly adjusting tilt angle and injection flow rate. A phase diagram of flow regimes highlights the interplay between gravitational, inertial and viscous forces critical to successful filling. Our findings show that slow, gravity-assisted injections minimize bypass and spillover, enabling targeted aneurysm filling even in complex geometries. CFD simulations highlight the need for patient-specific planning of such a procedure accounting for personal vascular anatomy. This strategy may provide a safer and less invasive alternative to current endovascular techniques, especially for anatomically inaccessible aneurysms. CI - (c) 2026 The Authors. FAU - Mekler, Tirosh AU - Mekler T AUID- ORCID: 0009-0008-7921-7064 AD - Department of Biomedical Engineering, Technion-Israel Institute of Technology , Haifa, Israel. FAU - Khoury, Maria AU - Khoury M AD - Department of Biomedical Engineering, Technion-Israel Institute of Technology , Haifa, Israel. FAU - Kreinin, Yevgeniy AU - Kreinin Y AD - Department of Biomedical Engineering, Technion-Israel Institute of Technology , Haifa, Israel. FAU - Fishler, Rami AU - Fishler R AD - Department of Biomedical Engineering, Technion-Israel Institute of Technology , Haifa, Israel. FAU - Natan, Nir AU - Natan N AD - Department of Biomedical Engineering, Technion-Israel Institute of Technology , Haifa, Israel. FAU - Bessler, Ron AU - Bessler R AD - Department of Biomedical Engineering, Technion-Israel Institute of Technology , Haifa, Israel. FAU - Leshansky, Alexander AU - Leshansky A AD - Department of Chemical Engineering, Technion-Israel Institute of Technology , Haifa, Israel. FAU - Sznitman, Josue AU - Sznitman J AUID- ORCID: 0000-0001-8217-3842 AD - Department of Biomedical Engineering, Technion-Israel Institute of Technology , Haifa, Israel. FAU - Korin, Netanel AU - Korin N AUID- ORCID: 0000-0001-7244-889X AD - Department of Biomedical Engineering, Technion-Israel Institute of Technology , Haifa, Israel. AD - Fischell Department of Bioengineering, University of Maryland , College Park, MD, USA. LA - eng GR - 101002057/European Research Council (ERC)/ PT - Journal Article PL - England TA - J R Soc Interface JT - Journal of the Royal Society, Interface JID - 101217269 SB - IM MH - *Intracranial Aneurysm/physiopathology/therapy MH - Humans MH - *Models, Cardiovascular MH - *Gravitation MH - Hydrodynamics MH - Computer Simulation PMC - PMC13714385 OTO - NOTNLM OT - aneurysm embolization OT - computational fluid dynamics OT - gravity-driven flow OT - injectable biomaterials OT - intracranial aneurysm COIS- We declare we have no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/28 CRDT- 2026/10/28 01:23 PHST- 2025/09/25 00:00 [received] PHST- 2026/08/27 00:00 [accepted] PHST- 2026/08/24 00:00 [revised] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:23 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 483895 [pii] AID - rsif20250968 [pii] AID - 10.1098/rsif.2025.0968 [doi] PST - ppublish SO - J R Soc Interface. 2026 Oct 7;23(243):20250968. doi: 10.1098/rsif.2025.0968. PMID- 42947368 OWN - NLM STAT- Publisher LR - 20261028 IS - 1537-2197 (Electronic) IS - 0002-9122 (Linking) DP - 2026 Oct 27 TI - Floral traits and hermaphrodite abundance shape female advantage in a gynodioecious plant. LID - e70282 [pii] LID - 10.1002/ajb2.70282 [doi] AB - PREMISE: Gynodioecy is maintained through female advantage in seed fitness that depends on the sex ratio of a population. We hypothesized that females of Silene acaulis would exhibit floral traits associated with elevated outcross pollination and that sex ratio would impact pollen limitation and inbreeding depression such that female advantage is greatest at high hermaphrodite frequency. METHODS: We examined how sex ratio affects female advantage by pairing observations of floral morphology and natural pollination with a pollination experiment in two sites that differed in sex ratio. We described the population structure of sex ratio by comparing site sex ratio to local scale sex ratio within 1 m of focal plants. RESULTS: We found evidence for frequency dependence of female advantage through pollination. As local hermaphrodite abundance increased, females were more likely to receive pollen and set fruit. Sex ratio was spatially structured, and only local sex ratio had significant effects on reproductive processes. Both sexes were similarly severely pollen limited, but longer stigmas on female flowers increased chances of pollen deposition. Hermaphrodites experienced inbreeding depression expressed during seed germination, but inbreeding did not fully explain female advantage. CONCLUSIONS: We confirm female reproductive advantage in a gynodioecious species and show that it is partly facilitated by superior pollination-mediated seed production due to longer stigmas which maximizes pollen receipt. Female advantage exhibits negative frequency dependence locally, such that female advantage is greatest when nearby hermaphrodites are abundant. These results advance our understanding of links between population reproductive processes and sexual selection. CI - (c) 2026 The Author(s). American Journal of Botany published by Wiley Periodicals LLC on behalf of Botanical Society of America. FAU - Chandler, Emma K AU - Chandler EK AUID- ORCID: 0000-0002-6507-1091 AD - University of Georgia, Department of Plant Biology, The University of Georgia, Athens, 30602-7271, GA, USA. FAU - DeMarche, Megan L AU - DeMarche ML AUID- ORCID: 0000-0002-5010-2721 AD - University of Georgia, Department of Plant Biology, The University of Georgia, Athens, 30602-7271, GA, USA. LA - eng GR - Botanical Society of America/ GR - 2413626/Division of Environmental Biology/ PT - Journal Article DEP - 20261027 PL - United States TA - Am J Bot JT - American journal of botany JID - 0370467 SB - IM OTO - NOTNLM OT - Caryophyllaceae OT - Silene OT - female advantage OT - frequency dependence OT - gynodioecy OT - inbreeding depression OT - pollen limitation OT - population sex structure OT - sex ratio OT - sexual dimorphism EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 01:23 PHST- 2026/08/28 00:00 [revised] PHST- 2026/05/15 00:00 [received] PHST- 2026/08/31 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:23 [entrez] AID - e70282 [pii] AID - 10.1002/ajb2.70282 [doi] PST - aheadofprint SO - Am J Bot. 2026 Oct 27:e70282. doi: 10.1002/ajb2.70282. PMID- 42947366 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1941-5923 (Electronic) IS - 1941-5923 (Linking) VI - 27 DP - 2026 Oct 28 TI - HHV-6 Encephalitis Presenting With Severe SIADH and CO(2) Narcosis in Non-Transplant Smoldering Adult T-Cell Leukemia/Lymphoma. PG - e954380 LID - 10.12659/AJCR.954380 [doi] AB - BACKGROUND Human herpesvirus 6 (HHV-6) encephalitis is best recognized after allogeneic hematopoietic stem cell transplantation but can also occur in non-transplant immunocompromised patients. Hyponatremia and syndrome of inappropriate antidiuretic hormone secretion (SIADH) may precede or accompany HHV-6 encephalitis. Severe SIADH as an early dominant clue in non-transplant patients with smoldering adult T-cell leukemia/lymphoma (ATLL) is not fully described. CASE REPORT A 55-year-old woman with smoldering ATLL received systemic corticosteroid therapy for clinically diagnosed human T-cell leukemia virus type 1 (HTLV-1)-associated uveitis and was admitted for progressive pulmonary infection. Bronchoscopic specimens revealed polymicrobial infection, including Nocardia and Actinomyces; invasive pulmonary aspergillosis was also clinically suspected. Although pulmonary findings improved with antimicrobial and antifungal therapy, she developed nausea, acute impairment of consciousness, and severe SIADH-associated hyponatremia. Serum sodium decreased to 113 mmol/L, and consciousness did not adequately improve after sodium correction. Cerebrospinal fluid obtained by lumbar puncture on hospital day 13 showed HHV-6 DNA positivity (1.0x10(3) copies/mL). Brain magnetic resonance imaging demonstrated bilateral cortical and limbic abnormalities, supporting a clinical diagnosis of HHV-6 encephalitis. Ganciclovir was initiated on hospital day 15 along with sodium correction, tolvaptan, and supportive care. The patient subsequently developed hypercapnic respiratory failure and CO(2) narcosis requiring mechanical ventilation. Her neurologic and respiratory status gradually improved, and she was discharged on hospital day 53. CONCLUSIONS In non-transplant patients with ATLL, corticosteroid exposure, and opportunistic infection, severe SIADH accompanied by new neurologic symptoms can be an early clue to HHV-6 encephalitis. Early recognition and prompt initiation of antiviral therapy may help to improve neurologic outcomes. FAU - Tateishi, Kana AU - Tateishi K AD - Department of Respiratory Medicine, Fukuoka University Hospital, Fukuoka, Japan. FAU - Yanagihara, Toyoshi AU - Yanagihara T AUID- ORCID: 0000-0002-2212-0631 AD - Department of Respiratory Medicine, Fukuoka University Hospital, Fukuoka, Japan. FAU - Kawabata, Ryoma AU - Kawabata R AD - Department of Respiratory Medicine, Fukuoka University Hospital, Fukuoka, Japan. FAU - Kinoshita, Eriko AU - Kinoshita E AD - Department of Respiratory Medicine, Fukuoka University Hospital, Fukuoka, Japan. FAU - Miyazaki, Takeshi AU - Miyazaki T AD - Department of Neurology, Fukuoka University Hospital, Fukuoka, Japan. FAU - Tateishi, Yuji AU - Tateishi Y AD - Department of Neurology, Fukuoka University Hospital, Fukuoka, Japan. FAU - Koga, Kaori AU - Koga K AD - Department of Pathology, Fukuoka University Hospital, Fukuoka, Japan. FAU - Hamasaki, Makoto AU - Hamasaki M AD - Department of Pathology, Fukuoka University Hospital, Fukuoka, Japan. FAU - Fujita, Masaki AU - Fujita M AUID- ORCID: 0000-0003-1209-1272 AD - Department of Respiratory Medicine, Fukuoka University Hospital, Fukuoka, Japan. LA - eng PT - Case Reports PT - Journal Article DEP - 20261028 PL - United States TA - Am J Case Rep JT - The American journal of case reports JID - 101489566 SB - IM MH - Humans MH - Female MH - Middle Aged MH - *Herpesvirus 6, Human MH - *Roseolovirus Infections/diagnosis/complications MH - *Leukemia-Lymphoma, Adult T-Cell/complications MH - *Inappropriate ADH Syndrome/etiology/diagnosis MH - *Encephalitis, Viral/diagnosis/complications EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 01:22 PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:22 [entrez] AID - 954380 [pii] AID - 10.12659/AJCR.954380 [doi] PST - epublish SO - Am J Case Rep. 2026 Oct 28;27:e954380. doi: 10.12659/AJCR.954380. PMID- 42947350 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1526-9914 (Electronic) IS - 1526-9914 (Linking) VI - 27 IP - 11 DP - 2026 Nov TI - Multi-system evaluation of baseline-referenced gray scale CNR profile deviation for phantom-based ultrasound quality assurance. LID - e70880 [pii] LID - 10.1002/acm2.70880 [doi] AB - BACKGROUND: Phantom-based ultrasound quality assurance (QA) commonly incorporates qualitative image assessment, whereas robust baseline-referenced quantitative indicators for routine monitoring across multiple systems remain limited. PURPOSE: To investigate whether controlled transducer surface acoustic shielding produces measurable baseline-referenced changes in selected dead zone (DZ) and gray scale (GS) indicators across multiple ultrasound systems using an ATS-539 phantom. METHODS: Four ultrasound systems (GE LOGIQ P10, GE LOGIQ P6, Mindray DC-70 Exp, and Siemens X150) were evaluated. The transducer aperture was partially shielded with insulating tape at 0%, 5%, 10%, 15%, 20%, and 30% coverage, applied either centrally or at the indicator-side margin. Five repeated images were acquired for each condition. GS performance was assessed using the contrast-to-noise ratio (CNR) of six cylindrical targets. Target-specific CNR values were converted to within-device z-deviations relative to baseline (L0), and the root-mean-square z-deviation across all six targets (RMS-z) was used as the primary GS profile indicator. DZ performance was evaluated using landmark-based pin brightness as a secondary near-field indicator. RESULTS: Under central shielding, the GS RMS-z profile deviation score increased with shielding ratio in all four systems (Spearman rho = 0.642-0.975, all p < 0.001). Central shielding produced consistent RMS-z increases across all four systems, whereas indicator-side shielding yielded a more heterogeneous response: RMS-z remained positively associated with shielding ratio in three systems (rho = 0.734-0.917, all p < 0.001), but the response was weaker and non-significant in the GE LOGIQ P10. Central DZ pin brightness decreased with increasing shielding ratio across all systems (rho = -0.581 to -0.865, all p < 0.001). Baseline CNR profiles and absolute CNR magnitudes differed substantially among systems, and within-device baseline referencing was therefore preferred over direct cross-system comparison. Manual landmark placement demonstrated high repeatability for target-specific CNR and DZ brightness measurements (ICC(A,1) = 0.88-0.97), whereas the aggregate RMS-z score showed moderate-to-good repeatability (ICC(A,1) = 0.771). CONCLUSIONS: Baseline-referenced GS CNR profile deviation demonstrated a consistent response to controlled acoustic shielding across multiple ultrasound systems. DZ brightness provided complementary near-field information but remained a secondary indicator. These findings support device-specific longitudinal monitoring of phantom-based QA metrics rather than interpretation based on universal cutoff values. CI - (c) 2026 The Author(s). Journal of Applied Clinical Medical Physics published by Wiley Periodicals, LLC on behalf of The American Association of Physicists in Medicine. FAU - Kim, Ji-Hye AU - Kim JH AUID- ORCID: 0009-0001-5484-4861 AD - Department of Radiological Science, Chungbuk Health & Science University, Cheongju-si, Chungcheongbuk-do, Republic of Korea. FAU - Heo, Yeong-Cheol AU - Heo YC AUID- ORCID: 0000-0003-2938-8531 AD - Department of Radiological Science, College of Health Science, Eulji University, Seongnam-si, Gyeonggi-do, Republic of Korea. LA - eng GR - 2026-0132/Eulji University/ PT - Evaluation Study PT - Journal Article PL - United States TA - J Appl Clin Med Phys JT - Journal of applied clinical medical physics JID - 101089176 SB - IM MH - *Phantoms, Imaging MH - *Quality Assurance, Health Care/standards MH - Ultrasonography/instrumentation/standards/methods MH - Humans MH - Signal-To-Noise Ratio MH - *Image Processing, Computer-Assisted/methods MH - *Transducers MH - Quality Control PMC - PMC13714345 OTO - NOTNLM OT - ATS-539 phantom OT - baseline-referenced monitoring OT - contrast-to-noise ratio OT - transducer surface acoustic shielding OT - ultrasound quality assurance COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/27 CRDT- 2026/10/28 01:12 PHST- 2026/10/05 00:00 [revised] PHST- 2026/07/09 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:12 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e70880 [pii] AID - ACM270880 [pii] AID - 10.1002/acm2.70880 [doi] PST - ppublish SO - J Appl Clin Med Phys. 2026 Nov;27(11):e70880. doi: 10.1002/acm2.70880. PMID- 42947343 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 2450-6303 (Electronic) IS - 1509-409X (Linking) VI - 28 IP - 2 DP - 2026 Jan 1 TI - Influence of the allocation and force of distraction springs on the head shape of a child with sagittal craniosynostosis. PG - 150-163 LID - 10.37190/abb/225306 [doi] AB - PURPOSE: This study presents the application of numerical methods, for preoperative planning and determining the force and placement of distraction springs, as well as the cranial incision sites, in a newborn with a congenital disability involving cranial suture fusion. METHODS: This study employs a multifaceted methodology based on the work of an interdisciplinary team. The procedure involves: (1) patient-specific finite element method numerical analysis to simulate varying distraction spring forces and allocations, (2) non-invasive 3D optical surface scanning for the precise analysis of post-operative anatomical changes, and (3) clinical verification of the numerical predictions. RESULTS: Numerical studies were verified against clinical trials, resulting in substantial changes in the head shape of a 4-month-old child, an expansion of the external cranial vault, and a measurable improvement in the cephalic index and overall skull geometry. CONCLUSION: Based on this single-patient numerical case study, it is hypothesized that the design of craniosynostosis treatment planning can be configured to introduce personalized force into critical areas. The proposed alternative spring allocation suggests potential biomechanical advantages, including stress reduction and improved shape optimization. However, these findings serve as a proof-of-concept and require validation in larger clinical trials. Achieving a clinical effect that is sustained over time by reducing stress values can also be significant, because the bone remodeling process can be designed so that bones multiply along the direction of principal stresses, thereby allowing for the optimization of the skull shape envisioned by the neurosurgeon. CI - (c) 2026 Arkadiusz Szarek et al., published by Wroclaw University of Science and Technology. FAU - Szarek, Arkadiusz AU - Szarek A AD - Department of Technology and Automation, Faculty of Mechanical Engineering and Computer Science, Czestochowa University of Technology, 42-201, Czestochowa, Poland. FAU - Paszta, Piotr AU - Paszta P AD - Department of Technology and Automation, Faculty of Mechanical Engineering and Computer Science, Czestochowa University of Technology, 42-201, Czestochowa, Poland. FAU - Larysz, Dawid AU - Larysz D AD - Department of Head and Neck Surgery for Children and Adolescents, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland. AD - Department of Head and Neck Surgery for Children and Adolescents, Prof. St. Popowski Regional Specialized Children's Hospital, Olsztyn, Poland. FAU - Larysz, Patrycja AU - Larysz P AD - Department of Head and Neck Surgery for Children and Adolescents, Prof. St. Popowski Regional Specialized Children's Hospital, Olsztyn, Poland. LA - eng PT - Journal Article DEP - 20261027 PL - Poland TA - Acta Bioeng Biomech JT - Acta of bioengineering and biomechanics JID - 101194794 SB - IM MH - Humans MH - *Craniosynostoses/surgery/diagnostic imaging/physiopathology/pathology MH - Finite Element Analysis MH - Infant MH - *Head/pathology/diagnostic imaging MH - *Osteogenesis, Distraction/instrumentation/methods MH - Biomechanical Phenomena MH - Skull/surgery MH - Stress, Mechanical MH - Imaging, Three-Dimensional OTO - NOTNLM OT - 3D head shapes OT - Craniosynostosis OT - Finite element modeling OT - Pre-operative planning OT - Spring assisted posterior vault expansion EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 01:03 PHST- 2026/04/25 00:00 [received] PHST- 2026/07/01 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:03 [entrez] AID - abb-225306 [pii] AID - 10.37190/abb/225306 [doi] PST - epublish SO - Acta Bioeng Biomech. 2026 Oct 27;28(2):150-163. doi: 10.37190/abb/225306. eCollection 2026 Jan 1. PMID- 42947341 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1526-9914 (Electronic) IS - 1526-9914 (Linking) VI - 27 IP - 11 DP - 2026 Nov TI - Impact of robust optimization and beam configuration on the distribution stability of LET under delivery uncertainties in carbon-ion therapy. LID - e70878 [pii] LID - 10.1002/acm2.70878 [doi] AB - BACKGROUND: Carbon-ion radiotherapy exploits high linear energy transfer (LET) to enhance biological effectiveness, and low dose-averaged LET (LETd) within the target volume is associated with local recurrence in pancreatic cancer. Although robust optimization is widely used to improve dose robustness against delivery uncertainties, systematic investigation of LET robustness across optimization strategies and beam configurations remains scarce. PURPOSE: To evaluate the robustness of LET metrics in carbon-ion therapy under delivery uncertainties using a phantom-based design, comparing planning target volume (PTV)-based with robust optimization and assessing the impact of seven beam configurations, and to characterize the differential response of LET and clinical dose (relative biological effectiveness [RBE]-weighted dose) metrics. METHODS: A 30 x 30 x 30 cm(3) water-equivalent phantom with rod- and C-shaped target geometries was planned in RayStation 12A with 50 Gy (RBE) in 10 fractions. Section 1 used two opposing lateral fields to compare PTV-based optimization (1, 3, 5 mm margins) with robust optimization incorporating translation, density, and combined uncertainties. Section 2 evaluated seven beam configurations, ranging from two-field to four-field arrangements, under PTV-based (3 mm) and robust (+/- 3%/3 mm) optimization. LETd98%, LETd mean, and LETd2% were extracted for the clinical target volume and organs at risk (OARs). Robustness was quantified using averaged interquartile range (AVG.IQR) and sensitivity slopes from 1% to 5% density uncertainty (S1-->5), and configurations were ranked by minimal-change and biologically favorable criteria. Spot weight distributions were analyzed to examine the mechanistic basis of the observed differences. RESULTS: Density-related uncertainties dominated LET variability, with LETd2% the most sensitive metric (AVG.IQR up to 40% for C-shaped targets). Robust optimization with combined translation-density parameters achieved lower variability than PTV-based optimization. LET and RBE-weighted dose metrics responded divergently, with the LETd mean increasing while the mean clinical dose decreased under density perturbations. Beam direction significantly affected LETd2% variability (p < 0.001 for all configurations). Two lateral fields (2Lat) ranked highest under the minimal-change criterion, whereas four-field oblique (4FO) with robust optimization ranked first under the biologically favorable criterion, balancing increased target LET against reduced OAR exposure. Spot weight analysis showed that, for the rod-shaped target, only robustness scenarios including density uncertainty used more energy layers and shifted weight away from distal layers (distal-third weight fraction 56.4-57.4% vs. 60.8% for PTV-based optimization); this pattern was not reproduced for the C-shaped target. CONCLUSIONS: Density uncertainties predominantly govern LET variability in carbon-ion therapy, with LETd2% the most sensitive indicator, and redistribution of spot weight away from distal energy layers provides a plausible mechanism. Beam configuration selection should reflect clinical priorities: 4FO with robust optimization when biological effectiveness is prioritized, and 2Lat for physical LET stability. These phantom-based findings support explicit LET robustness evaluation in carbon-ion treatment planning and warrant validation in patient studies. CI - (c) 2026 The Author(s). Journal of Applied Clinical Medical Physics published by Wiley Periodicals, LLC on behalf of The American Association of Physicists in Medicine. FAU - Phongprapun, Wisawa AU - Phongprapun W AD - Department of Medical Physics for Heavy Ion Therapy, Graduate School of Medicine, Gunma University, Maebashi, Gunma, Japan. FAU - Tashiro, Mutsumi AU - Tashiro M AUID- ORCID: 0000-0003-3253-0527 AD - Department of Medical Physics for Heavy Ion Therapy, Graduate School of Medicine, Gunma University, Maebashi, Gunma, Japan. AD - Gunma University Heavy Ion Medical Center, Maebashi, Gunma, Japan. LA - eng PT - Journal Article PL - United States TA - J Appl Clin Med Phys JT - Journal of applied clinical medical physics JID - 101089176 SB - IM MH - Humans MH - *Radiotherapy Planning, Computer-Assisted/methods MH - *Heavy Ion Radiotherapy/methods MH - Radiotherapy Dosage MH - *Linear Energy Transfer MH - *Phantoms, Imaging MH - *Pancreatic Neoplasms/radiotherapy MH - *Organs at Risk/radiation effects MH - Uncertainty MH - Relative Biological Effectiveness MH - Radiotherapy, Intensity-Modulated/methods MH - Algorithms PMC - PMC13714341 OTO - NOTNLM OT - carbon-ion radiotherapy OT - linear energy transfer OT - low-dose-averaged LET OT - phantom-based study OT - planning target volume COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/27 CRDT- 2026/10/28 01:03 PHST- 2026/08/17 00:00 [revised] PHST- 2026/06/21 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 01:03 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e70878 [pii] AID - ACM270878 [pii] AID - 10.1002/acm2.70878 [doi] PST - ppublish SO - J Appl Clin Med Phys. 2026 Nov;27(11):e70878. doi: 10.1002/acm2.70878. PMID- 42947336 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2001-1326 (Electronic) IS - 2001-1326 (Linking) VI - 16 IP - 11 DP - 2026 Nov TI - Selective AUTAC-mediated ATP7B degradation unlocks cuproptosis vulnerability in pancreatic ductal adenocarcinoma. LID - e70848 [pii] LID - 10.1002/ctm2.70848 [doi] AB - Cuproptosis arises from mitochondrial copper overload, yet how tumour cells evade this metabolic vulnerability remains unclear. We identify ATP7B as the central barrier to cuproptosis in pancreatic ductal adenocarcinoma (PDAC). ATP7B is aberrantly upregulated in PDAC and predicts poor prognosis. Its depletion increases intracellular copper, activates FDX1, DLAT/DLST lipoylation, disrupts tricarboxylic acid (TCA) metabolism, and reinstates cuproptosis in vitro and in vivo. Mechanistically, ATP7B undergoes ZBTB10-driven K63-linked ubiquitination, recognized by NBR1 for selective autophagic degradation. Leveraging this, we developed an ATP7B-targeting autophagy-targeting chimera (ATAUTAC) that promotes ATP7B clearance, amplifies copper-driven mitochondrial metabolic perturbations, triggers cuproptosis, and inhibits tumour growth with favourable tolerability. Notably, ATAUTAC sensitizes PDAC to gemcitabine, overcoming resistance by exposing latent metabolic fragility. These findings establish ATP7B as a mechanistic hub of cuproptosis tolerance and demonstrate that autophagy-based ATP7B degradation is a druggable strategy for copper-dependent tumour eradication. ATAUTAC offers a promising avenue for precision therapy in PDAC and other refractory malignancies. CI - (c) 2026 The Author(s). Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics. FAU - Zhang, Zihan AU - Zhang Z AD - Department of Interventional Radiology, Zhongshan Hospital, Fudan University, Shanghai, China. FAU - Li, Min AU - Li M AD - Department of Interventional Radiology, Zhongshan Hospital, Fudan University, Shanghai, China. FAU - Feng, Jiale AU - Feng J AD - Department of Pancreatic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China. FAU - Li, Jian-Ang AU - Li JA AUID- ORCID: 0000-0002-6532-8811 AD - Department of Pancreatic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China. FAU - Zhang, Lei AU - Zhang L AUID- ORCID: 0000-0002-7517-1192 AD - Department of Pancreatic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China. LA - eng PT - Journal Article PL - United States TA - Clin Transl Med JT - Clinical and translational medicine JID - 101597971 RN - EC 7.2.2.8 (Copper-Transporting ATPases) RN - 789U1901C5 (Copper) SB - IM MH - Humans MH - *Cuproptosis MH - *Carcinoma, Pancreatic Ductal/drug therapy/metabolism/genetics MH - Animals MH - *Pancreatic Neoplasms/drug therapy/metabolism MH - *Copper-Transporting ATPases/metabolism/genetics MH - Mice MH - Autophagy/drug effects MH - *Copper/metabolism MH - Cell Line, Tumor PMC - PMC13714097 OTO - NOTNLM OT - ATP7B OT - cuproptosis OT - drug development OT - pancreatic ductal adenocarcinoma OT - targeted therapies COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/27 CRDT- 2026/10/28 00:54 PHST- 2026/09/01 00:00 [revised] PHST- 2025/12/23 00:00 [received] PHST- 2026/09/11 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:54 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e70848 [pii] AID - CTM270848 [pii] AID - 10.1002/ctm2.70848 [doi] PST - ppublish SO - Clin Transl Med. 2026 Nov;16(11):e70848. doi: 10.1002/ctm2.70848. PMID- 42947335 OWN - NLM STAT- In-Process LR - 20261029 IS - 1939-9170 (Electronic) IS - 0012-9658 (Linking) VI - 107 IP - 10 DP - 2026 Oct TI - Erratum to "Soil microbes mediate the effects of resource variability on plant invasion". LID - e70555 [pii] LID - 10.1002/ecy.70555 [doi] LA - eng PT - Published Erratum PL - United States TA - Ecology JT - Ecology JID - 0043541 SB - IM EFR - Ecology. 2023 Oct;104(10):e4154. doi: 10.1002/ecy.4154. PMID: 37611168 EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:54 PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:54 [entrez] AID - e70555 [pii] AID - 10.1002/ecy.70555 [doi] PST - ppublish SO - Ecology. 2026 Oct;107(10):e70555. doi: 10.1002/ecy.70555. PMID- 42947327 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2001-1326 (Electronic) IS - 2001-1326 (Linking) VI - 16 IP - 11 DP - 2026 Nov TI - Predicting cardiovascular disease in peritoneal dialysis patients using glycated proteomics and machine learning: A prospective multicentre study. LID - e70889 [pii] LID - 10.1002/ctm2.70889 [doi] AB - BACKGROUND: Cardiovascular disease (CVD) is the leading cause of death among peritoneal dialysis (PD) patients, yet PD-specific biomarkers and prediction models remain limited. This study aimed to identify glycated peptide biomarkers associated with CVD in PD patients and develop a prediction model integrating glycated proteomics and clinical indicators using machine learning. METHODS: In Phase I, a matched discovery cohort (48 PD patients with CVD and 48 without CVD) was used to identify glycated peptides by liquid chromatography-tandem mass spectrometry (LC-MS/MS), with glmnet used to prioritise candidate biomarkers. In Phase II, candidate biomarkers were quantified in a prospective internal derivation cohort (n = 240) and a multicentre external validation cohort (n = 102). The combined model was developed using F-test feature selection and elastic-net logistic regression. Its predictive performance was compared with three published cardiovascular risk models. Patients were stratified using a training-derived Youden cutoff. RESULTS: In the discovery cohort, 257 glycated peptides were differentially abundant between groups. Four candidate biomarkers were prioritised, and two peptides, GEAFTLK(g)ATVLNYLPK and K(g)QHLFVK, were retained in the final model together with six clinical indicators: phosphate, diabetes, total Kt/V, albumin, cardiac troponin T, and glycated albumin. The combined model achieved an area under the receiver operating characteristic curve (ROC-AUC) of 0.784 in the training set, 0.781 in the internal testing set, and 0.822 in the external validation cohort, respectively. Using the training-derived cutoff of 0.373, higher 1-year CVD incidence was observed in the high-risk group in both validation cohorts. The combined model also achieved higher ROC-AUCs than the published cardiovascular risk models. CONCLUSION: We developed and externally validated a PD-specific CVD prediction model by integrating glycated peptide biomarkers with clinical indicators, which may facilitate earlier risk stratification and targeted prevention. CI - (c) 2026 The Author(s). Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics. FAU - Ji, Ji AU - Ji J AUID- ORCID: 0009-0007-0472-0477 AD - Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China. AD - Shanghai Medical Center of Kidney, Shanghai, China. AD - Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China. AD - Shanghai Institute of Kidney and Dialysis, Shanghai, China. FAU - Nie, Zhaoying AU - Nie Z AD - Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China. AD - Shanghai Medical Center of Kidney, Shanghai, China. AD - Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China. AD - Shanghai Institute of Kidney and Dialysis, Shanghai, China. FAU - Jiang, Na AU - Jiang N AD - Department of Nephrology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China. FAU - Xie, Ting AU - Xie T AD - Division of Nephrology, Minhang Hospital, Fudan University, Shanghai, China. FAU - Zhang, Lei AU - Zhang L AD - Department of Nephrology, Shanghai Baoshan District Wusong Central Hospital (Wusong Branch, Zhongshan Hospital Affiliated to Fudan University), Shanghai, China. FAU - Liu, Xiaoyan AU - Liu X AD - Department of Nephrology, Second Hospital, Dalian Medical University, Dalian, China. FAU - Lu, Min AU - Lu M AD - Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China. AD - Shanghai Medical Center of Kidney, Shanghai, China. AD - Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China. AD - Shanghai Institute of Kidney and Dialysis, Shanghai, China. FAU - Xiang, Bo AU - Xiang B AD - Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China. AD - Shanghai Medical Center of Kidney, Shanghai, China. AD - Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China. AD - Shanghai Institute of Kidney and Dialysis, Shanghai, China. FAU - Wu, Weiwei AU - Wu W AD - Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China. AD - Shanghai Medical Center of Kidney, Shanghai, China. AD - Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China. AD - Shanghai Institute of Kidney and Dialysis, Shanghai, China. FAU - Jiang, Xiaotian AU - Jiang X AD - Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China. AD - Shanghai Medical Center of Kidney, Shanghai, China. AD - Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China. AD - Shanghai Institute of Kidney and Dialysis, Shanghai, China. FAU - Zhang, Lin AU - Zhang L AD - Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China. AD - Shanghai Medical Center of Kidney, Shanghai, China. AD - Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China. AD - Shanghai Institute of Kidney and Dialysis, Shanghai, China. FAU - Ji, Jun AU - Ji J AD - Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China. AD - Shanghai Medical Center of Kidney, Shanghai, China. AD - Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China. AD - Shanghai Institute of Kidney and Dialysis, Shanghai, China. FAU - Zou, Jianzhou AU - Zou J AD - Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China. AD - Shanghai Medical Center of Kidney, Shanghai, China. AD - Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China. AD - Shanghai Institute of Kidney and Dialysis, Shanghai, China. FAU - Ding, Xiaoqiang AU - Ding X AD - Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China. AD - Shanghai Medical Center of Kidney, Shanghai, China. AD - Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China. AD - Shanghai Institute of Kidney and Dialysis, Shanghai, China. FAU - Lu, Haojie AU - Lu H AD - Department of Chemistry, Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai, China. AD - Institutes of Biomedical Sciences and NHC Key Laboratory of Glycoconjugates Research, Fudan University, Shanghai, China. FAU - Yu, Xiaofang AU - Yu X AD - Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai, China. AD - Shanghai Medical Center of Kidney, Shanghai, China. AD - Shanghai Key Laboratory of Kidney and Blood Purification, Shanghai, China. AD - Shanghai Institute of Kidney and Dialysis, Shanghai, China. LA - eng GR - 2025YFC2511800/National Key Research and Development Program of China/ GR - 82270772/National Natural Science Foundation of China/ PT - Journal Article PT - Multicenter Study PL - United States TA - Clin Transl Med JT - Clinical and translational medicine JID - 101597971 RN - 0 (Biomarkers) RN - 0 (Glycation End Products, Advanced) RN - 0 (Glycated Serum Albumin) SB - IM MH - Humans MH - *Cardiovascular Diseases/diagnosis/etiology/epidemiology MH - Female MH - *Peritoneal Dialysis/adverse effects/methods/statistics & numerical data MH - *Proteomics/methods MH - *Machine Learning MH - Prospective Studies MH - Biomarkers/blood/analysis MH - Male MH - Middle Aged MH - Aged MH - Predictive Learning Models MH - Glycation End Products, Advanced MH - ROC Curve MH - Glycated Serum Albumin PMC - PMC13714096 OTO - NOTNLM OT - cardiovascular disease OT - glycated proteomics OT - machine learning OT - peritoneal dialysis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/27 CRDT- 2026/10/28 00:54 PHST- 2026/10/14 00:00 [revised] PHST- 2026/08/03 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:54 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e70889 [pii] AID - CTM270889 [pii] AID - 10.1002/ctm2.70889 [doi] PST - ppublish SO - Clin Transl Med. 2026 Nov;16(11):e70889. doi: 10.1002/ctm2.70889. PMID- 42947323 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2001-1326 (Electronic) IS - 2001-1326 (Linking) VI - 16 IP - 11 DP - 2026 Nov TI - Piezo1-mediated unfolded protein response in goblet cells regulates intestinal mucus barrier homeostasis through the Ca(2) (+)-IRE1alpha-XBP1 pathway. LID - e70886 [pii] LID - 10.1002/ctm2.70886 [doi] AB - BACKGROUND: Our previous study revealed that Piezo1 in colonic goblet cells senses mechanical stimuli to regulate the production and secretion of MUC2, but the underlying mechanism remains unclear. Altered endoplasmic reticulum function and the unfolded protein response (UPR), which are critical for MUC2 protein folding and secretion, were observed in goblet cell-specific Piezo1 knockout mice. This study aimed to investigate whether and how Piezo1 in goblet cells modulates mucus barrier function by regulating the UPR. METHODS: In vitro, LS174T cells were subjected to orbital shaking to simulate fluid shear stress (FSS) stimulation. The expression of UPR-related molecules and MUC2 was detected using real-time PCR, Western blotting, and immunofluorescence staining in the presence or absence of Piezo1 and UPR inhibition. In vivo, goblet cell-specific Piezo1 knockout (cKO) mice were treated with dextran sulphate sodium (DSS) and water avoidance stress (WAS). We analysed changes in goblet cell morphology, mucus layer thickness, UPR-related protein expression and ER structure. In addition, DSS-induced colitis mice were intraperitoneally injected with Yoda1 to activate Piezo1, and alterations in pathological structures and mucus layer thickness were subsequently evaluated. RESULTS: Compared with PERK or ATF6, FSS activated PIEZO1 and promoted calcium influx, thereby maintaining ER calcium homeostasis and selectively activating the IRE1alpha-XBP1 signalling pathway. This cascade facilitated MUC2 production, whereas pharmacological inhibition of PIEZO1 or IRE1alpha expression significantly abolished these effects. Compared with WT mice, cKO mice exhibited aggravated mucus barrier impairment, downregulated IRE1alpha-XBP1 and aggravated ER swelling under DSS and WAS. In contrast, pharmacological activation of Piezo1 via Yoda1 treatment restored intestinal mucus layer thickness and ameliorated colitis in DSS mice. CONCLUSIONS: Piezo1 senses mechanical stimulation to maintain ER calcium homeostasis and modulates the UPR via the IRE1alpha-XBP1 pathway, thereby facilitating MUC2 production in goblet cells and maintaining intestinal mucosal barrier homeostasis. KEY POINTS: Piezo1 in colonic goblet cells senses intestinal mechanical stimulation and modulates endoplasmic reticulum calcium homeostasis via Ca(2+) influx. The Piezo1-Ca(2+)-IRE1alpha-XBP1 signaling axis controls Mucin2 synthesis and production to maintain intestinal mucus barrier integrity. Pharmacological activation of Piezo1 by Yoda1 rescues mucus barrier defects, providing a potential therapeutic strategy for gastrointestinal mucosal injury. CI - (c) 2026 The Author(s). Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics. FAU - Wu, Jiandi AU - Wu J AUID- ORCID: 0000-0002-5239-3286 AD - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. FAU - Wang, Shaotong AU - Wang S AD - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. FAU - Wang, Zhe AU - Wang Z AUID- ORCID: 0009-0000-0558-5210 AD - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. FAU - Fang, Jianhua AU - Fang J AD - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. FAU - Li, Xueyan AU - Li X AD - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. FAU - Zhang, Qingqing AU - Zhang Q AUID- ORCID: 0009-0001-2620-7603 AD - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. FAU - Dai, Yan AU - Dai Y AD - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. FAU - Tian, Wenjing AU - Tian W AD - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. FAU - Ma, Yize AU - Ma Y AD - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. FAU - Li, Gangping AU - Li G AD - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. FAU - Zhang, Lei AU - Zhang L AD - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. FAU - Jiang, Yudong AU - Jiang Y AUID- ORCID: 0000-0001-5783-539X AD - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. FAU - Hou, Xiaohua AU - Hou X AUID- ORCID: 0000-0002-3694-2126 AD - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. FAU - Song, Jun AU - Song J AUID- ORCID: 0000-0002-0255-4547 AD - Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. LA - eng GR - 2022YFC250400/National Key Research and Development Program of China/ GR - 82370548/National Natural Science Foundation of China/ GR - 82200615/National Natural Science Foundation of China/ PT - Journal Article PL - United States TA - Clin Transl Med JT - Clinical and translational medicine JID - 101597971 RN - EC 2.7.11.1 (Protein Serine-Threonine Kinases) RN - 0 (Ion Channels) RN - 0 (X-Box Binding Protein 1) RN - 0 (Piezo1 protein, mouse) RN - SY7Q814VUP (Calcium) RN - EC 3.1.- (Endoribonucleases) RN - 0 (Xbp1 protein, mouse) RN - EC 2.7.11.1 (Ern1 protein, mouse) SB - IM MH - Animals MH - *Goblet Cells/metabolism MH - *Unfolded Protein Response/physiology/genetics MH - Mice MH - *Protein Serine-Threonine Kinases/metabolism MH - Homeostasis MH - *Ion Channels/metabolism/genetics MH - *X-Box Binding Protein 1/metabolism/genetics MH - Intestinal Barrier Function MH - *Calcium/metabolism MH - Mice, Knockout MH - *Endoribonucleases/metabolism MH - *Mucus/metabolism MH - Signal Transduction MH - Humans MH - *Intestinal Mucosa/metabolism MH - Colitis PMC - PMC13714353 OTO - NOTNLM OT - IRE1alpha OT - Piezo1 OT - XBP1 OT - dextran sulphate sodium OT - mucus barrier OT - unfolded protein response OT - water avoidance stress COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/27 CRDT- 2026/10/28 00:53 PHST- 2026/10/09 00:00 [revised] PHST- 2026/04/02 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:53 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e70886 [pii] AID - CTM270886 [pii] AID - 10.1002/ctm2.70886 [doi] PST - ppublish SO - Clin Transl Med. 2026 Nov;16(11):e70886. doi: 10.1002/ctm2.70886. PMID- 42947318 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1462-2920 (Electronic) IS - 1462-2912 (Linking) VI - 28 IP - 10 DP - 2026 Oct TI - Comprehensive Microbiome Profiling of Cheese Production Environments Using Long-Read Sequencing. LID - e70461 [pii] LID - 10.1111/1462-2920.70461 [doi] AB - Environmental microbiota in cheese-production facilities can influence both product quality and hygienic conditions. This study characterised airborne and surface-associated microbial communities in production and ripening rooms of eight Slovak bryndza cheese facilities using culture-dependent methods and long-amplicon Nanopore sequencing. Air samples were collected with PBS-filled impingers and surface-swab suspensions were processed for DNA extraction. Bacterial 16S rRNA and fungal 28S rRNA gene amplicons were sequenced and taxonomically classified using Kraken2 and EMU. Community analyses included alpha-diversity estimates, Bray-Curtis ordination, PERMANOVA and supplementary ALDEx2 comparisons. Microbial loads varied among facilities and rooms, with elevated bacterial or fungal counts detected in several ripening environments. Sequencing revealed diverse bacterial and fungal communities comprising ripening-associated microorganisms, environmental contaminants, potential spoilage organisms and low-abundance taxa of hygienic relevance. Surface material had limited effects on overall bacterial and fungal community composition, although selected differences in fungal richness were observed among materials and individual facilities showed distinct microbial profiles. To our knowledge, this is among the first studies applying long-amplicon Nanopore sequencing to jointly characterise airborne and surface-associated bacterial and fungal communities in dairy-production environments. These findings support integrated environmental monitoring that considers microbial load, community diversity and facility-specific production conditions. CI - (c) 2026 John Wiley & Sons Ltd. FAU - Klistincova, Nikola AU - Klistincova N AUID- ORCID: 0009-0001-6829-6309 AD - Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia. FAU - Farkas, Zuzana AU - Farkas Z AD - Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia. FAU - Buckova, Maria AU - Buckova M AD - Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia. FAU - Puskarova, Andrea AU - Puskarova A AD - Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia. FAU - Kvalova, Katarina AU - Kvalova K AD - Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia. FAU - Plany, Matej AU - Plany M AUID- ORCID: 0000-0002-7758-6383 AD - Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia. AD - Department of Animal Production, Division of Beekeeping, National Agricultural and Food Centre, Liptovsky Hradok, Slovakia. FAU - Pavlovic, Jelena AU - Pavlovic J AUID- ORCID: 0009-0006-4821-961X AD - Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia. FAU - Korenova, Janka AU - Korenova J AD - Department of Microbiology and Molecular Biology, Division of Food Science, National Agricultural and Food Centre, Bratislava, Slovakia. FAU - Kuchta, Tomas AU - Kuchta T AD - Department of Microbiology and Molecular Biology, Division of Food Science, National Agricultural and Food Centre, Bratislava, Slovakia. FAU - Pangallo, Domenico AU - Pangallo D AUID- ORCID: 0000-0002-9115-9409 AD - Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia. AD - Department of Biology, Institute of Biology and Biotechnology, Faculty of Natural Sciences, University of Ss. Cyril and Methodius in Trnava, Trnava, Slovakia. LA - eng GR - APVV-20-0001/Slovak Research and Development Agency/ PT - Journal Article PL - England TA - Environ Microbiol JT - Environmental microbiology JID - 100883692 RN - 0 (RNA, Ribosomal, 16S) RN - 0 (RNA, Ribosomal, 28S) SB - IM MH - *Cheese/microbiology MH - *Microbiota/genetics MH - *Bacteria/classification/genetics/isolation & purification MH - *Fungi/classification/genetics/isolation & purification MH - RNA, Ribosomal, 16S/genetics MH - *Air Microbiology MH - RNA, Ribosomal, 28S/genetics OTO - NOTNLM OT - DNA sequencing OT - air OT - cheese facility OT - microbial load OT - microbiota OT - monitoring OT - surface EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 00:53 PHST- 2026/07/23 00:00 [revised] PHST- 2025/12/11 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:53 [entrez] AID - e70461 [pii] AID - 10.1111/1462-2920.70461 [doi] PST - ppublish SO - Environ Microbiol. 2026 Oct;28(10):e70461. doi: 10.1111/1462-2920.70461. PMID- 42947316 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1098-2752 (Electronic) IS - 0738-1085 (Linking) VI - 46 IP - 7 DP - 2026 Oct TI - Secondary Mastopexy in the Reconstructed Ptotic Breast: Insights on Nipple Areolar Preservation and Complications. LID - e70304 [pii] LID - 10.1002/micr.70304 [doi] AB - INTRODUCTION: Secondary mastopexy is frequently performed following nipple-sparing mastectomy (NSM) with deep inferior epigastric perforator (DIEP) flap breast reconstruction to address ptosis and optimize aesthetic outcomes. Preservation of the nipple-areolar complex (NAC) during secondary mastopexy remains a concern, particularly in ptotic breasts. This study aims to describe a single surgeon's experience with secondary mastopexy and to examine factors that may impact complication rates. METHODS: This retrospective institutional review examines patients with grade 2 or 3 ptosis who received secondary mastopexy following NSM with immediate or delayed DIEP flap reconstruction. All patients underwent peri-areolar incision design with preservation of an inferior dermal pedicle, and operative perfusion assessment was performed primarily with indocyanine green (ICG). Complications were recorded and grouped as minor or major events. Exploratory breast-level comparisons were performed for selected risk factors. RESULTS: Eleven patients with 21 breasts were included. Mastectomy incisions included L-shaped (n = 9), inframammary (n = 10), and Wise pattern (n = 2) incisions. Ten of the eleven patients underwent delayed reconstruction. Five breasts received radiation before mastopexy. Complications occurred in nine breasts, most commonly minor wound dehiscence (n = 4) and NAC epidermolysis/discoloration (n = 2). No cases of full-thickness NAC necrosis occurred. In exploratory analysis, complications were more frequent after therapeutic than prophylactic NSM per patient (7/8 [87.5%] vs. 0/3 [0%], p = 0.02). However, this correlation was not significant at the breast level (5/8 [62.5%] vs. 4/13 [30.8], p = 0.20). Prior radiation showed a borderline association with complications per patient (5/5 [100%] vs. 2/6 [33.3%], p = 0.06). However, this correlation was not significant at the breast level (2/5 [40.0%] vs. 6/15 [40.0], p = 1.00). CONCLUSION: Nipple-preserving secondary mastopexy following NSM and DIEP flap reconstruction is supported in selected patients, with preservation of NAC viability and no observed cases of necrosis in this series. Larger, multi-institutional studies are needed to better define patient selection, timing, and the effect of prior oncologic treatment on outcomes. CI - (c) 2026 Wiley Periodicals LLC. FAU - Darras, Osama AU - Darras O AUID- ORCID: 0000-0002-5051-0581 AD - Cleveland Clinic Foundation, Cleveland, Ohio, USA. AD - Department of Surgery, Oregon Health and Science University, Portland, Oregon, USA. FAU - Jensen, Kate AU - Jensen K AUID- ORCID: 0009-0007-1996-4474 AD - Cleveland Clinic Foundation, Cleveland, Ohio, USA. FAU - Phuyal, Diwakar AU - Phuyal D AUID- ORCID: 0000-0001-7967-2210 AD - Cleveland Clinic Foundation, Cleveland, Ohio, USA. FAU - Gurunian, Raffi AU - Gurunian R AUID- ORCID: 0000-0001-9271-5983 AD - Cleveland Clinic Foundation, Cleveland, Ohio, USA. AD - Department of Plastic Surgery, Cleveland Clinic Abu Dhabi, Abu Dhabi, UAE. FAU - Bishop, Sarah N AU - Bishop SN AUID- ORCID: 0000-0002-7660-7576 AD - Cleveland Clinic Foundation, Cleveland, Ohio, USA. LA - eng PT - Journal Article PL - United States TA - Microsurgery JT - Microsurgery JID - 8309230 SB - IM MH - Humans MH - Female MH - *Mammaplasty/methods/adverse effects MH - Retrospective Studies MH - *Nipples/surgery MH - Adult MH - *Postoperative Complications/etiology MH - Middle Aged MH - *Perforator Flap/transplantation/blood supply MH - *Breast Neoplasms/surgery/pathology MH - Treatment Outcome MH - Mastectomy/methods OTO - NOTNLM OT - mastectomy OT - mastopexy OT - nipple EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 00:53 PHST- 2026/07/24 00:00 [revised] PHST- 2026/01/16 00:00 [received] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:53 [entrez] AID - e70304 [pii] AID - 10.1002/micr.70304 [doi] PST - ppublish SO - Microsurgery. 2026 Oct;46(7):e70304. doi: 10.1002/micr.70304. PMID- 42947315 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1473-2300 (Electronic) IS - 0300-0605 (Linking) VI - 54 IP - 10 DP - 2026 Oct TI - Development and external validation of an interpretable machine learning model for predicting 4-year incident frailty in middle-aged and older adults with arthritis: A retrospective longitudinal study based on the CHARLS and KLoSA cohorts. PG - 3000605261497564 LID - 10.1177/03000605261497564 [doi] AB - ObjectiveBased on retrospective longitudinal data from the China Health and Retirement Longitudinal Study (CHARLS) and Korean Longitudinal Study of Ageing (KLoSA) cohorts, we developed and externally validated an interpretable machine learning model to predict 4-year incident frailty in middle-aged and older adults with arthritis.MethodsModel development was performed using the CHARLS cohort (n = 2126) and included participants aged >/=45 years who had been diagnosed with arthritis and were nonfrail at their initial survey. New-onset frailty was tracked over a 4-year interval. Thirty-one candidate predictors were identified through a literature review, none of which overlapped with the deficits constituting the frailty index. Ten core features, including age, marital status, left- and right-hand grip strength, number of painful sites, knee pain, leg pain, back pain, number of hospitalizations, and antirheumatic medication use, were selected via combined least absolute shrinkage and selection operator-Boruta feature selection. Six algorithms were compared based on their area under the curves in the internal validation dataset, specificity, sensitivity, and other clinical applicability metrics. Tenfold cross-validation was employed. The selected model was frozen and transported to KLoSA without refitting. A prespecified recalibration-in-the-large was applied to account for the lower baseline frailty incidence in the Korean cohort. Model interpretability was achieved with SHapley Additive exPlanations global and local analyses, and an online calculator was developed.ResultsLogistic regression was selected as the final model because it achieved competitive discrimination with the least shrinkage between training and validation sets. Its discriminative ability, measured as the area under the receiver operating characteristic curve, was 0.764 (95% confidence interval: 0.707-0.816) internally and remained at 0.747 (95% confidence interval: 0.701-0.793) when the model was transported to KLoSA. Performance was stable across 10-fold cross-validation (mean, 0.772 +/- 0.046). Discrimination was well preserved, although a prespecified recalibration-in-the-large was required to correct for the lower baseline frailty incidence in the Korean cohort (Brier score 0.205-->0.065). In the SHapley Additive exPlanations ranking, the number of painful sites, antirheumatic medication use, leg pain, age, and grip strength contributed the most. Once a user enters the 10 easily collected variables, the calculator reports that person's probability of becoming frail within 4 years. In post hoc analyses, pain burden and low grip strength emerged as two independent risk dimensions. High pain burden remained associated with falls (adjusted odds ratio = 1.60) and hospitalization (1.66) even at a given level of frailty, whereas low grip strength and medication use were associated with an increased frailty risk without a corresponding excess of events.ConclusionsThis explainable machine learning model, developed using data from Chinese middle-aged and older patients with arthritis and externally validated in a KLoSA cohort, combines acceptable predictive accuracy with cross-population robustness. The tool estimates an individualized 4-year frailty risk probability using 10 routinely available indicators. Given the observational nature of both cohorts, this model should be viewed as a candidate risk stratification aid rather than a validated clinical tool. Formal impact evaluation through prospective validation is necessary before the model can be recommended for use in community settings. FAU - Xu, Xiao AU - Xu X AD - Research Center of Molecular Medicine (PhD Lab), Faculty of Nursing, Nantong Health College of Jiangsu Province (Nantong Health Vocational College), China. RINGGOLD: 541296 FAU - Cesar, Nihimbazwe AU - Cesar N AD - National Institute of Public Health (Institut National de Sante Publique, INSP), Burundi. RINGGOLD: 278022 FAU - Ntawuyamara, Epipode AU - Ntawuyamara E AUID- ORCID: 0009-0005-1704-0716 AD - Department of Medicine, Kamenge Teaching Hospital (Centre Hospitalo-Universitaire de Kamenge), University of Burundi, Burundi. RINGGOLD: 107788 FAU - Su, Su-Hua AU - Su SH AD - Department of Rheumatology, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), China. RINGGOLD: 74723 LA - eng PT - Journal Article PT - Validation Study DEP - 20261027 PL - England TA - J Int Med Res JT - The Journal of international medical research JID - 0346411 SB - IM MH - Humans MH - *Frailty/epidemiology/diagnosis MH - Longitudinal Studies MH - Retrospective Studies MH - *Machine Learning MH - Male MH - Middle Aged MH - Female MH - Predictive Learning Models MH - Aged MH - *Arthritis/complications/epidemiology/diagnosis/physiopathology MH - China/epidemiology MH - Republic of Korea/epidemiology MH - Incidence OTO - NOTNLM OT - Arthritis OT - artificial intelligence OT - frailty OT - machine learning OT - precision medicine COIS- Declaration of conflicting interestsThe authors declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 00:53 PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:53 [entrez] AID - 10.1177/03000605261497564 [doi] PST - ppublish SO - J Int Med Res. 2026 Oct;54(10):3000605261497564. doi: 10.1177/03000605261497564. Epub 2026 Oct 27. PMID- 42947293 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1942-5473 (Electronic) IS - 0164-1263 (Linking) VI - 48 IP - 5 DP - 2026 Sep 15 TI - Acceptability, Feasibility, and Satisfaction of Buzzy((R))and Vibradent(TM) for Pain and Anxiety Management in Children During Local Anesthesia Administration. PG - 344-351 AB - Purpose: This study evaluated the efficacy of 2 vibrotactile devices, Buzzy((R)) and Vibradent(TM), in reducing pain and anxiety during local anesthesia (LA) administration for dental extractions in children. Methods: The study included 126 children aged 7 to 10 years, divided into 3 groups (N=42 each): Group 1 (conventional syringe), Group 2 (Vibradent (TM)), and Group 3 (Buzzy((R))). Pain was assessed using the Wong-Baker Faces Pain Rating Scale (WBFPS), Face-Legs-Activity-Cry-Consolability scale (FLACC), and Sound-Eye-Motor scale (SEM) at baseline (T0), needle insertion (Tn), and end of local anesthesia administration (Te). Anxiety was measured through the Facial-Image-Scale (FIS) and heart rate. Salivary cortisol was evaluated at T0 and T20 (20 minutes after needle insertion) to assess physiological stress. Parent and child satisfaction were also recorded. Results: Both Buzzy((R)) and Vibradent(TM) significantly reduced pain, anxiety, and salivary cortisol compared to the control group (P<0.001). Buzzy((R)) showed more favorable outcomes in WBFPS, FLACC, SEM, heart rate, and FIS scores (P<0.05) and the highest satisfaction ratings. Both devices showed significantly lower salivary cortisol levels (P<0.05), with no significant difference between them. Conclusions: Buzzy((R)) and Vibradent (TM) are effective, non-invasive techniques for reducing pain, anxiety, and physiological stress. Buzzy((R)) demonstrated superior overall efficacy. FAU - Nabi, Shajaratul Yakeen AU - Nabi SY AD - Department of Pediatric and Preventive Dentistry, Government Dental College and Hospital, Srinagar, Jammu and Kashmir, India. FAU - Shah, Aaqib AU - Shah A AD - Senior resident, Division of Conservative Dentistry and Endodontics, Centre for Dental Education and Research, All India Institute of Medical Sciences (AIIMS), New Delhi, India. FAU - Lone, Nazia AU - Lone N AD - Department of Pediatric and Preventive Dentistry, Government Dental College and Hospital, Srinagar, Jammu and Kashmir, India. LA - eng PT - Journal Article PT - Randomized Controlled Trial PL - United States TA - Pediatr Dent JT - Pediatric dentistry JID - 7909102 RN - 0 (Anesthetics, Local) RN - WI4X0X7BPJ (Hydrocortisone) SB - IM MH - Humans MH - Child MH - Female MH - Male MH - *Anesthesia, Local/instrumentation MH - Pain Measurement MH - Patient Satisfaction MH - *Dental Anxiety/prevention & control MH - Feasibility Studies MH - *Vibration/therapeutic use MH - Tooth Extraction MH - *Anesthesia, Dental/instrumentation MH - *Pain Management/instrumentation MH - Anesthetics, Local/administration & dosage MH - Hydrocortisone/analysis MH - *Anxiety/prevention & control MH - Pediatric Anesthesia EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 00:49 PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:49 [entrez] PST - ppublish SO - Pediatr Dent. 2026 Sep 15;48(5):344-351. PMID- 42947288 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1099-1069 (Electronic) IS - 0278-0232 (Linking) VI - 44 IP - 6 DP - 2026 Nov TI - Baseline Monoclonal Protein Identifies Plasmacytic Differentiation and Shorter Progression-Free Survival in Marginal Zone Lymphoma. LID - e70286 [pii] LID - 10.1002/hon.70286 [doi] AB - Monoclonal protein is frequently tested in marginal zone lymphoma, but interpretation is often inconsistent in practice because prior studies used selective testing and rarely linked serologic findings to biology or post-treatment dynamics. We analyzed 235 adults with newly diagnosed MZL receiving first-line systemic therapy at a single center (2010-2025). All patients had baseline serum protein electrophoresis, immunofixation, and free light chain testing. Plasmacytic differentiation was reviewed by hematopathologists. M-protein response (IMWG criteria) was assessed in patients with post-treatment data. The primary endpoint was progression-free survival (PFS). Baseline M-protein was detected in 117 of 235 patients (49.8%), most commonly immunoglobulin M (IgM) (65.8%). M-protein positivity was associated with a higher frequency of plasmacytic differentiation (39.6% vs. 16.2%; adjusted OR 3.78). M-protein-positive patients had shorter PFS than M-protein-negative patients (median, 50.9 months vs. not reached; log-rank p = 0.044), and this association remained in multivariable analysis (hazard ratio [HR] 1.58). Overall survival (OS), progression of disease within 24 months (POD24), and histologic transformation did not differ significantly by M-protein status. Among 70 patients with evaluable post-treatment data, M-protein response of at least partial response (PR) was highly specific for clinical response and had a high positive predictive value, but response depth did not predict PFS. In uniformly tested MZL patients requiring systemic therapy, baseline M-protein identifies a subgroup enriched for plasmacytic differentiation and associated with shorter PFS. Post-treatment M-protein decline can support response confirmation when present, but it should not be used as a surrogate endpoint for durable disease control without prospective validation. CI - (c) 2026 John Wiley & Sons Ltd. All rights reserved, including rights for text and data mining and training of artificial intelligence technologies or similar technologies. FAU - Wang, Wei AU - Wang W AUID- ORCID: 0000-0002-1192-4896 AD - Department of Hematology, Peking Union Medical College Hospital, Beijing, China. FAU - Zhao, Danqing AU - Zhao D AD - Department of Hematology, Peking Union Medical College Hospital, Beijing, China. FAU - Yang, Chen AU - Yang C AD - Department of Hematology, Peking Union Medical College Hospital, Beijing, China. FAU - Zhang, Lu AU - Zhang L AUID- ORCID: 0000-0002-0860-9625 AD - Department of Hematology, Peking Union Medical College Hospital, Beijing, China. FAU - Zhang, Wei AU - Zhang W AUID- ORCID: 0000-0001-9489-5965 AD - Department of Hematology, Peking Union Medical College Hospital, Beijing, China. FAU - Zhou, Daobin AU - Zhou D AUID- ORCID: 0000-0002-1592-1932 AD - Department of Hematology, Peking Union Medical College Hospital, Beijing, China. FAU - Zhang, Yan AU - Zhang Y AUID- ORCID: 0000-0003-0244-218X AD - Department of Hematology, Peking Union Medical College Hospital, Beijing, China. FAU - Li, Jian AU - Li J AUID- ORCID: 0000-0002-4549-0694 AD - Department of Hematology, Peking Union Medical College Hospital, Beijing, China. LA - eng GR - 2024-2-4011/Capital's Funds for Health Improvement and Research/ GR - 2024-I2M-C&T-B-010/CAMS Innovation Fund for Medical Sciences/ GR - UBJ10659/Peking Union Medical College Hospital Outstanding Young Talent Development Program/ GR - 2025-PUMCH-D001/National High Level Hospital Clinical Research Funding/ PT - Journal Article PL - England TA - Hematol Oncol JT - Hematological oncology JID - 8307268 RN - 0 (Myeloma Proteins) SB - IM MH - Humans MH - *Lymphoma, B-Cell, Marginal Zone/mortality/pathology/blood/drug therapy MH - Female MH - Male MH - Middle Aged MH - Aged MH - Progression-Free Survival MH - *Plasma Cells/pathology/metabolism MH - *Cell Differentiation MH - Adult MH - Aged, 80 and over MH - Prognosis MH - *Myeloma Proteins/analysis OTO - NOTNLM OT - marginal zone lymphoma OT - monoclonal protein OT - plasmacytic differentiation OT - prognosis OT - progression-free survival EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 00:48 PHST- 2026/10/01 00:00 [revised] PHST- 2026/08/10 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:48 [entrez] AID - e70286 [pii] AID - 10.1002/hon.70286 [doi] PST - ppublish SO - Hematol Oncol. 2026 Nov;44(6):e70286. doi: 10.1002/hon.70286. PMID- 42947281 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1553-2712 (Electronic) IS - 1069-6563 (Linking) VI - 33 IP - 10 DP - 2026 Oct TI - Association of Initial Peripheral Venous pO(2) With 48-Hour Clinical Deterioration in Emergency Department Patients Undergoing Venous Blood Gas Testing. LID - e70429 [pii] LID - 10.1111/acem.70429 [doi] AB - BACKGROUND: Peripheral venous pO(2) is routinely reported with venous blood gas (VBG) results despite uncertainty regarding its prognostic significance. We evaluated whether initial peripheral venous pO(2) was associated with 48-h clinical deterioration and provided prognostic information beyond age and lactate. METHODS: We conducted an ambispective observational cohort study of adults undergoing VBG testing at a single urban academic emergency department, with prospective enrollment and index-data collection and retrospective ascertainment of 48-h outcomes. The primary outcome was a composite of ICU admission, death, vasopressor use, or positive-pressure ventilation within 48 h. The primary analysis modeled venous pO(2) continuously and used multivariable logistic regression adjusted for age and lactate. Incremental performance was evaluated exploratorily by comparing an age-and-lactate model with a model additionally containing venous pO(2). RESULTS: Among 973 patients, 103 (10.6%) experienced the composite outcome. Median venous pO(2) was 33 mmHg (IQR 25-44). Venous pO(2) was not associated with clinical deterioration after adjustment for age and lactate (adjusted odds ratio [aOR] 1.054 per 10-mmHg increase; 95% CI 0.957-1.161; p = 0.289). The AUROC was 0.649 for the age-and-lactate model and 0.654 after adding venous pO(2) (DeltaAUROC 0.005; DeLong p = 0.390). CONCLUSION: In this single-center cohort of adult ED patients undergoing VBG testing, no association was observed between peripheral venous pO(2) and 48-h clinical deterioration. Adding venous pO(2) did not improve apparent prognostic performance beyond age and lactate. We cannot exclude smaller associations or potential utility in selected disease-specific populations. Confirmation in larger multicenter cohorts using consecutive enrollment and external validation is needed. CI - (c) 2026 Society for Academic Emergency Medicine. FAU - Corbo, Jill AU - Corbo J AUID- ORCID: 0000-0001-8153-5709 AD - Jacobi Medical Center, Albert Einstein College of Medicine, Bronx, New York, USA. FAU - Gruber, Peter AU - Gruber P AD - Jacobi Medical Center, Albert Einstein College of Medicine, Bronx, New York, USA. FAU - Acosta, Nicholas AU - Acosta N AUID- ORCID: 0009-0005-6023-7661 AD - Jacobi Medical Center, Albert Einstein College of Medicine, Bronx, New York, USA. FAU - Palmer, Aunaly AU - Palmer A AD - Jacobi Medical Center, Albert Einstein College of Medicine, Bronx, New York, USA. AD - Washington University School of Medicine, Saint Louis, Missouri, USA. FAU - Friedman, Benjamin W AU - Friedman BW AUID- ORCID: 0000-0002-2753-5860 AD - Montefiore Medical Center, Albert Einstein College of Medicine, Bronx, New York, USA. LA - eng PT - Journal Article PT - Observational Study PL - United States TA - Acad Emerg Med JT - Academic emergency medicine : official journal of the Society for Academic Emergency Medicine JID - 9418450 RN - S88TT14065 (Oxygen) RN - 33X04XA5AT (Lactic Acid) SB - IM MH - Humans MH - *Emergency Service, Hospital MH - Female MH - *Blood Gas Analysis/methods MH - Middle Aged MH - Male MH - *Oxygen/blood MH - Prognosis MH - Retrospective Studies MH - Prospective Studies MH - Lactic Acid/blood MH - Adult MH - Aged MH - Veins MH - Logistic Models EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 00:48 PHST- 2026/10/01 00:00 [revised] PHST- 2026/06/16 00:00 [received] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:48 [entrez] AID - e70429 [pii] AID - 10.1111/acem.70429 [doi] PST - ppublish SO - Acad Emerg Med. 2026 Oct;33(10):e70429. doi: 10.1111/acem.70429. PMID- 42947269 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2056-9920 (Print) IS - 2056-9920 (Electronic) IS - 2056-9920 (Linking) VI - 12 IP - 1 DP - 2026 Jul 30 TI - Variable retinal presentations after retrobulbar needle perforation: a case series. LID - 10.1186/s40942-026-00897-0 [doi] LID - 143 AB - BACKGROUND: Retrobulbar anesthesia is widely used in ophthalmic surgery due to its rapid onset and effective akinesia. However, although uncommon, globe perforation remains one of its most severe complications and may lead to a wide spectrum of posterior segment damage. While incidence and risk factors have been extensively described, less attention has been given to the management of the resulting retinal and choroidal injuries. MAIN BODY: We report a retrospective case series of four patients with retinal or choroidal complications following retrobulbar needle perforation. Clinical presentations were heterogeneous, including proliferative vitreoretinopathy, subretinal hemorrhage, choroidal neovascularization, and submacular hemorrhage. Management was individualized according to the predominant retinal pathology and included pars plana vitrectomy-based approaches, subretinal recombinant tissue plasminogen activator, and intravitreal anti-vascular endothelial growth factor therapy. Anatomical stabilization was achieved in all cases, with visual outcomes appearing to be influenced by macular involvement and timing of intervention. CONCLUSION: Retrobulbar needle perforation can result in diverse retinal pathologies despite a common mechanism of injury. This small case series illustrates the importance of early recognition, multimodal imaging, and individualized management in selected complex cases. CI - (c) 2026. The Author(s). FAU - Juan, Eva Ramon AU - Juan ER AUID- ORCID: 0009-0003-8911-0598 AD - General Ophthalmology Department, IMO Grupo Miranza, Balmes, 322, Barcelona, 3 1. 08006, Spain. evaramonjuan@gmail.com. FAU - Oliver-Gutierrez, David AU - Oliver-Gutierrez D AD - Retina and Vitreoretinal Surgery Department, Hospital Universitari Bellvitge (HUB), Barcelona, Spain. FAU - Lorenzo-Arroyo, Alberto Salmoral AU - Lorenzo-Arroyo AS AD - General Ophthalmology Department, IMO Grupo Miranza, Balmes, 322, Barcelona, 3 1. 08006, Spain. FAU - Garcia-Arumi, Claudia AU - Garcia-Arumi C AD - Retina and vitreoretinal Surgery Department, IMO Grupo Miranza, Barcelona, Spain. AD - Retina and vitreoretinal Surgery Department, Hospital Universitari Vall d'Hebron (HUVH), Barcelona, Spain. FAU - Garcia-Arumi, Jose AU - Garcia-Arumi J AD - Retina and vitreoretinal Surgery Department, IMO Grupo Miranza, Barcelona, Spain. AD - Retina and vitreoretinal Surgery Department, Hospital Universitari Vall d'Hebron (HUVH), Barcelona, Spain. LA - eng PT - Journal Article PT - Review DEP - 20260730 PL - England TA - Int J Retina Vitreous JT - International journal of retina and vitreous JID - 101677897 PMC - PMC13715286 OTO - NOTNLM OT - Choroidal neovascularization OT - Globe perforation OT - Iatrogenic ocular trauma OT - Needle injury OT - Proliferative vitreoretinopathy OT - Retinal injury OT - Retinal perforation OT - Retrobulbar anesthesia OT - Submacular hemorrhage COIS- Declarations. Ethics approval and consent to participate: This study adhered to the tenets of the Declaration of Helsinki. Given its retrospective design and the use of anonymized clinical data, formal ethics committee approval was not required according to institutional policy. Written informed consent for the use of clinical data was obtained from all patients. Consent for publication: Written informed consent for publication of clinical data and accompanying images was obtained from all patients. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/30 CRDT- 2026/10/28 00:48 PHST- 2026/03/22 00:00 [received] PHST- 2026/06/28 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:48 [entrez] PHST- 2026/07/30 00:00 [pmc-release] AID - 10.1186/s40942-026-00897-0 [pii] AID - 897 [pii] AID - 10.1186/s40942-026-00897-0 [doi] PST - epublish SO - Int J Retina Vitreous. 2026 Jul 30;12(1):143. doi: 10.1186/s40942-026-00897-0. PMID- 42947267 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2072-1315 (Electronic) IS - 1606-0997 (Print) IS - 1606-0997 (Linking) VI - 45 IP - 1 DP - 2026 Jul 24 TI - Learning from implementation: health worker insights on strengthening immunisation data quality in Kampala's lower-level private-for-profit health sector following implementation of a data quality improvement intervention. LID - 10.1186/s41043-026-01399-3 [doi] LID - 239 AB - BACKGROUND: Lower-level private-for-profit health facilities are integral to expanding access to routine immunisation services within Uganda's urban health system. And yet, limited research has examined how these providers implement and sustain data quality improvement efforts amid resource constraints and competing profit motives. This qualitative inquiry examines provider experiences and insights following the implementation of a data quality improvement intervention, generating lessons to inform strategies for strengthening immunisation data quality within Kampala's lower-level private-for-profit health sector. METHODS: The endline assessment adopted a qualitative case study approach and was embedded within the Urban Immunisation Data Quality Improvement intervention. A purposively selected sample of twenty implementers took part in the study and were drawn from the lower-level private-for-profit immunisation health service providers in the two city divisions of Kawempe and Makindye. Monitoring visits and endline interviews informed the inquiry, where the interactions were audio recorded, later transcribed and thematically analysed using Atlas. ti following the Performance of Routine Information System Management (PRISM) framework which guided the deductive and inductive analytical approaches with anonymised quotes illuminating respondents' voices. RESULTS: Overall, results revealed that the co-designed intervention strengthened some existing data improvement strategies, such as the birth cohort registers, monitoring charts, and data review, thereby improving tracing, timeliness, and awareness. Yet the paper-based dependence in some health facilities, lack of equipment, and costs related to improvisation in pursuit of data quality persisted. While the support supervision fostered ownership, the understaffing and high staff turnover, and uneven leadership in some health facilities limited progress. Reporting and feedback improved overall but remained inconsistent, especially in health facilities not yet onboarded onto the DHIS2 system. CONCLUSIONS: While the co-designed intervention worked to improve data tracking and awareness, sustained data quality gains require stronger infrastructure, leadership, workforce stability, and embedded feedback-driven data use processes. CI - (c) 2026. The Author(s). FAU - Ssegujja, Eric AU - Ssegujja E AUID- ORCID: 0000-0002-7732-1019 AD - Department of Health Policy Planning and Management, School of Public Health, College of Health Sciences, Makerere University, Kampala, Uganda. ssega2001@yahoo.com. FAU - Kiggundu, Paul AU - Kiggundu P AD - Directorate of Public Health and Environment, Kampala Capital City Authority, Kampala, Uganda. FAU - Zalwango, Sarah AU - Zalwango S AD - Directorate of Public Health and Environment, Kampala Capital City Authority, Kampala, Uganda. FAU - Rutebemberwa, Elizeus AU - Rutebemberwa E AD - Department of Health Policy Planning and Management, School of Public Health, College of Health Sciences, Makerere University, Kampala, Uganda. LA - eng PT - Journal Article DEP - 20260724 PL - Bangladesh TA - J Health Popul Nutr JT - Journal of health, population, and nutrition JID - 100959228 SB - IM MH - Humans MH - Uganda MH - *Quality Improvement MH - *Immunization Programs/standards MH - *Private Sector MH - *Data Accuracy MH - *Health Personnel/psychology MH - Qualitative Research PMC - PMC13715189 OTO - NOTNLM OT - Data quality improvement OT - Health systems strengthening OT - Lower-level private-for-profit providers OT - Urban immunisation services COIS- Declarations. Ethics approval and consent to participate: Ethical approval for this study was conducted by the Ethics Committee of the Makerere University School of Public Health, Higher Degrees Research and Ethics Committee (HDREC) (ref: HDREC 848) and the Ugandan National Council for Sciences and Technology (UNCST) (ref: HS996ES). The study was conducted in accordance with the ethical principles outlined in the Declaration of Helsinki. All participants were informed about the purpose of the study, assured of confidentiality, and provided written consent prior to participation. Participation was voluntary, and respondents were informed that they could withdraw at any time without any consequence. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/24 CRDT- 2026/10/28 00:48 PHST- 2025/12/31 00:00 [received] PHST- 2026/06/30 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:48 [entrez] PHST- 2026/07/24 00:00 [pmc-release] AID - 10.1186/s41043-026-01399-3 [pii] AID - 1399 [pii] AID - 10.1186/s41043-026-01399-3 [doi] PST - epublish SO - J Health Popul Nutr. 2026 Jul 24;45(1):239. doi: 10.1186/s41043-026-01399-3. PMID- 42947262 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2524-4671 (Electronic) IS - 2524-4671 (Linking) VI - 8 IP - 1 DP - 2026 Jul 28 TI - Bacillus probiotic blend modulates ileal microbiome to improve growth performance and prevent necrotic enteritis in broiler chickens. LID - 10.1186/s42523-026-00606-0 [doi] LID - 109 AB - The intensification of poultry production has led to an increased prevalence of necrotic enteritis (NE), a costly disease caused by Clostridium perfringens, traditionally managed through antibiotic use. However, concerns over antibiotic resistance have driven the search for alternative solutions. This study evaluates the efficacy of a three-strain Bacillus probiotic blend as an alternative for preventing NE in broiler chickens. The three Bacillus strains, Bacillus subtilis ATCC PTA126786, B. velezensis ATCC PTA127064, and B. velezensis ATCC PTA 127065, were selected based on their probiotic attributes, especially their ability to inhibit the growth of C. perfringens and other poultry pathogens. Our results from in vivo experiments showed that dietary supplementation with the probiotic blend significantly reduced NE-associated mortality and improved growth performance metrics such as average daily gain and feed conversion ratio. Metagenomic analyses of ileal contents of chickens supplemented with the Bacillus blend revealed a significant reduction in the abundances of C. perfringens, Enterococcus cecorum, and Eimeria acervulina following NE challenge, despite modest changes in overall microbiome composition. Importantly, the observed probiotic efficacy was strongly associated with the Bacillus strains colonization in the gut, as measured by the Bacillus genome recovery in ileal content samples. These findings highlight the potential of Bacillus-based probiotics as an effective alternative to antibiotics in poultry production, contributing to improved animal health and sustainability. CI - (c) 2026. The Author(s). FAU - Baxter, Nielson AU - Baxter N AD - BiomEdit, Inc., Indianapolis, IN, USA. FAU - Plata, German AU - Plata G AD - BiomEdit, Inc., Indianapolis, IN, USA. FAU - Bown, Luke AU - Bown L AD - BiomEdit, Inc., Indianapolis, IN, USA. AD - Nucliq Biologics, Inc., St. John, NL, Canada. FAU - Shields, Jacob AU - Shields J AD - BiomEdit, Inc., Indianapolis, IN, USA. FAU - Augspurger, Nathan AU - Augspurger N AD - BiomEdit, Inc., Indianapolis, IN, USA. AD - United Soybean Board, Chesterfield, MO, USA. FAU - Kumar, Arvind AU - Kumar A AD - BiomEdit, Inc., Indianapolis, IN, USA. FAU - Susanti, Dwi AU - Susanti D AD - BiomEdit, Inc., Indianapolis, IN, USA. dsusanti@biomedit.com. LA - eng PT - Journal Article DEP - 20260728 PL - England TA - Anim Microbiome JT - Animal microbiome JID - 101759457 PMC - PMC13715241 OTO - NOTNLM OT - Bacillus OT - Chickens OT - Microbiome OT - Necrotic enteritis OT - Performance OT - Probiotics COIS- Declarations. Ethical approval: All experimental protocols and animal handling procedures were reviewed and approved by the Southern Poultry Feed & Research Institutional Animal Care and Use Committee (IACUC Approval Number: BE2022-010). Research was conducted in accordance with "The 8th edition of the Guide for Care and use of Laboratory Animals" (NRC, 2011). Chickens were reared following the Guide for the Care and use of Agricultural Animals in Agricultural Research and Teaching" (Federation of Animal Science Societies, 2010). Consent for publication: Not applicable. Competing interests: NB, GP, JS, AK, and DS are employees of BiomEdit, Inc., a company that researches and develops veterinary products. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/28 CRDT- 2026/10/28 00:47 PHST- 2026/03/19 00:00 [received] PHST- 2026/07/16 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:47 [entrez] PHST- 2026/07/28 00:00 [pmc-release] AID - 10.1186/s42523-026-00606-0 [pii] AID - 606 [pii] AID - 10.1186/s42523-026-00606-0 [doi] PST - epublish SO - Anim Microbiome. 2026 Jul 28;8(1):109. doi: 10.1186/s42523-026-00606-0. PMID- 42947258 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1478-6362 (Electronic) IS - 1478-6354 (Print) IS - 1478-6354 (Linking) VI - 28 IP - 1 DP - 2026 Jul 31 TI - Patterns of the gut microbiota and metabolome as potential biomarkers associated with influenza vaccine immunogenicity in systemic lupus erythematosus: a cross-sectional observational pilot study. LID - 10.1186/s13075-026-03875-x [doi] LID - 190 AB - BACKGROUND: Immunogenicity to influenza vaccination is often impaired in systemic lupus erythematosus (SLE). The role of the gut microbiota and its metabolic output in modulating this response remains underexplored. This study is the first to explore gut microbiota patterns and metabolites in response to influenza vaccination in patients with SLE. METHODS: A cross-sectional observational pilot study was conducted at Ramathibodi Hospital between May 2021 and July 2022. Participants with SLE who had received either a boosted dose or a standard dose of a quadrivalent influenza vaccine were enrolled. We analyzed fecal 16 S rDNA and metabolomic profiles using liquid chromatography-mass spectrometry to identify signatures associated with seroconversion (determined using the hemagglutination inhibition assay). RESULTS: A total of 62 participants with SLE participated in the study, and 61 fecal samples were submitted for metabolomic analysis. Most participants were female (58/62, 93.6%). The median ages in the standard and booster dose groups were 35.5 (interquartile range 28-42.5) and 34 (25-43) years, respectively (P = 0.878). The overall gut microbiota patterns were similar in both groups; however, the genus Bacteroides was significantly associated with seroconversion to A(H1N1) and A(H3N2) (P < 0.05). Metabolomic analysis identified upregulation of 1,5-anhydro-D-glucitol and L-cysteic acid in seroconverters, whereas neopterin was significantly elevated in nonresponders, furthermore, several bacterial taxa were correlated with these metabolites. The level of immunosuppressive treatment and the use of antimicrobials for prophylaxis against opportunistic infections affected influenza immunogenicity in this study population. Some metabolites also appeared to be linked to vaccine immunogenicity, although the underlying mechanism is unclear. CONCLUSIONS: Our findings identify specific gut microbial taxa and fecal metabolites, particularly 1,5-anhydro-D-glucitol, which are significantly associated with influenza vaccine immunogenicity in SLE patients. These results suggest that the gut-immune axis may play a modulatory role in vaccine responses and may provide potential non-invasive biomarkers that can identify patients at risk of suboptimal influenza protection. CI - (c) 2026. The Author(s). FAU - Kiewsanuan, Nathathai AU - Kiewsanuan N AD - Department of Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. FAU - Ngamjanyaporn, Pintip AU - Ngamjanyaporn P AD - Department of Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. FAU - Pisitkun, Prapaporn AU - Pisitkun P AD - Department of Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. FAU - Payungporn, Sunchai AU - Payungporn S AD - Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. AD - Center of Excellence in Systems Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. FAU - Sawaswong, Vorthon AU - Sawaswong V AD - Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. AD - Center of Excellence in Systems Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. FAU - Chanchaem, Prangwalai AU - Chanchaem P AD - Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. AD - Center of Excellence in Systems Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. FAU - Maiuthed, Arnatchai AU - Maiuthed A AD - Department of Pharmacology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand. AD - Centre of Biopharmaceutical Science for Healthy Ageing, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand. FAU - Thongpradit, Supranee AU - Thongpradit S AD - Research Center, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. FAU - Lohaphuntrakun, Napong AU - Lohaphuntrakun N AD - Department of Pharmacology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand. FAU - Juntranggoor, Pichakorn AU - Juntranggoor P AD - Department of Pharmacology, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand. FAU - Rotjanapan, Porpon AU - Rotjanapan P AUID- ORCID: 0000-0003-0966-2708 AD - Department of Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. porpon.rot@mahidol.ac.th. AD - Division of Infectious Diseases, Department of Medicine, Faculty of Medicine Ramathibodi Hospital, Mahidol University, 270 Rama VI Road, Ratchathewi, Bangkok, 10400, Thailand. porpon.rot@mahidol.ac.th. LA - eng GR - RF_64108/Mahidol University/ PT - Journal Article PT - Observational Study DEP - 20260731 PL - England TA - Arthritis Res Ther JT - Arthritis research & therapy JID - 101154438 RN - 0 (Influenza Vaccines) RN - 0 (Biomarkers) SB - IM MH - Humans MH - Female MH - *Influenza Vaccines/immunology MH - *Lupus Erythematosus, Systemic/immunology/metabolism MH - Pilot Projects MH - Cross-Sectional Studies MH - Male MH - Adult MH - *Gastrointestinal Microbiome/immunology MH - Biomarkers/metabolism/analysis MH - *Metabolome/immunology MH - *Influenza, Human/prevention & control/immunology MH - *Immunogenicity, Vaccine/immunology MH - Feces/microbiology PMC - PMC13715192 OTO - NOTNLM OT - Gut microbiota OT - Immunogenicity OT - Influenza vaccine OT - Metabolomic profile OT - SLE COIS- Declarations. Ethics approval and consent to participate: This study was conducted according to the Declaration of Helsinki of 1964 and its later amendments and was approved by the Institutional Review Board of Ramathibodi Hospital (approval no. MURA2021/132). The committee approved the use of patient samples and data for the publication of this study. Written consent was obtained from all participants. Consent for publication: Not applicable. Competing interest: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:47 PHST- 2026/03/23 00:00 [received] PHST- 2026/07/25 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:47 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s13075-026-03875-x [pii] AID - 3875 [pii] AID - 10.1186/s13075-026-03875-x [doi] PST - epublish SO - Arthritis Res Ther. 2026 Jul 31;28(1):190. doi: 10.1186/s13075-026-03875-x. PMID- 42947248 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1756-994X (Electronic) IS - 1756-994X (Linking) VI - 18 IP - 1 DP - 2026 Jul 30 TI - Benchmarking and optimisation of bait-capture metagenomics for sequencing of respiratory viruses at scale. LID - 10.1186/s13073-026-01725-8 [doi] LID - 166 AB - BACKGROUND: Sequencing respiratory virus genomes is essential for public health surveillance and research. Although shotgun metagenomics is pathogen agnostic, its sensitivity is limited by abundant off-target host nucleic acids. Hybridization bait capture overcomes this limitation by selectively enriching viral sequences prior to sequencing. METHODS: We evaluated three respiratory viral bait capture workflows (veSEQ, RVI-seq, and Illumina) to compare their performance and assess their suitability for scalable respiratory virus sequencing. Synthetic RNA controls and clinical samples containing SARS-CoV-2, influenza A, influenza B, human parainfluenza virus, and respiratory syncytial virus (RSV) were analysed. RESULTS: All three workflows demonstrated high efficiency, reproducibility and broadly comparable performance across respiratory viruses. Complete viral genomes were consistently recovered from samples containing 10,000 viral copies, while viral reads remained detectable at substantially lower viral loads, including approximately 100 copies. Workflow optimisation reduced reagent costs and enabled laboratory automation without compromising sequencing sensitivity. CONCLUSION: Hybridization bait capture provides an effective and scalable approach for respiratory virus genome sequencing. Cost reductions and automation can be implemented without compromising performance, supporting its use in routine genomic surveillance and public health preparedness. CI - (c) 2026. The Author(s). FAU - Wagner, Josef AU - Wagner J AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. jw43@sanger.ac.uk. FAU - Rajan, Diana AU - Rajan D AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Field, Sarah Frances AU - Field SF AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Betteridge, Emma AU - Betteridge E AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Bellis, Katie AU - Bellis K AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Knoll, Marissa AU - Knoll M AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Ng, Duncan AU - Ng D AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Teixeira, Diego AU - Teixeira D AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Blane, Beth AU - Blane B AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Akram, Asha AU - Akram A AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - de Sousa, Catarina AU - de Sousa C AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Brennan, Joe AU - Brennan J AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Aggarwal, Dinesh AU - Aggarwal D AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. AD - Department of Infectious Disease, Imperial College London, London, W2 1PG, UK. FAU - MacIntyre-Cockett, George AU - MacIntyre-Cockett G AD - Nuffield Department of Medicine, Centre for Human Genetics, Roosevelt Dr, Headington, Oxford, OX3 7BN, UK. FAU - Chaudron, Sandra E AU - Chaudron SE AD - Nuffield Department of Medicine, Centre for Human Genetics, Roosevelt Dr, Headington, Oxford, OX3 7BN, UK. FAU - Grayson, Nicholas AU - Grayson N AD - Nuffield Department of Medicine, Centre for Human Genetics, Roosevelt Dr, Headington, Oxford, OX3 7BN, UK. FAU - Hyatt, Ben AU - Hyatt B AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Wong, Andrew AU - Wong A AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Galvin, Anastasia AU - Galvin A AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Huang, Ya-Lin AU - Huang YL AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Jackson, David AU - Jackson D AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Forbes, Matthew AU - Forbes M AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Schwach, Frank AU - Schwach F AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Frick-Kretschmer, Andrea AU - Frick-Kretschmer A AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Figueroa, Katerina AU - Figueroa K AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Lassalle, Florent AU - Lassalle F AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Roberts-Sengier, William AU - Roberts-Sengier W AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Lignes, Adrianne AU - Lignes A AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Novaes, Fernanda AU - Novaes F AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Fatima, Salma AU - Fatima S AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Howe, Kevin AU - Howe K AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Stott, Sara AU - Stott S AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. FAU - Bonsall, David AU - Bonsall D AD - Nuffield Department of Medicine, Centre for Human Genetics, Roosevelt Dr, Headington, Oxford, OX3 7BN, UK. AD - Nuffield Department of Medicine, Pandemic Sciences Institute, University of Oxford, Old Road Campus, Oxford, OX3 7DQ, UK. FAU - Harrison, Ewan M AU - Harrison EM AD - Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, CB10 1SA, UK. eh6@sanger.ac.uk. AD - Department of Medicine, University of Cambridge, Addenbrooke's Hospital, Cambridge, CB2 0QQ, UK. eh6@sanger.ac.uk. LA - eng GR - 220540/Z/20/A/Wellcome/ GR - Quinquennial Review 2021-2026'/Wellcome Sanger Institute/ PT - Journal Article DEP - 20260730 PL - England TA - Genome Med JT - Genome medicine JID - 101475844 SB - IM MH - *Metagenomics/methods MH - Humans MH - *Genome, Viral MH - Benchmarking MH - High-Throughput Nucleotide Sequencing/methods MH - SARS-CoV-2/genetics MH - Reproducibility of Results MH - Workflow PMC - PMC13715170 COIS- Declarations. Ethics approval and consent of participate: Respiratory Virus and Microbiome Initiative (RVI) received ethics committee approval from the National Research Ethics Service Committee London-Surrey Research Ethics Committee, 12/09/2022, REC reference: 22/PR/1222, IRAS project ID: 316530. Additional healthy nasal swabs were collected anonymously from volunteer staff members of the Wellcome Sanger Institute after approval of sampling methodology and participant information by the Research Governance team and the Chair of the Sanger Institute and Connecting Science Research Ethics Committee (SICS-REC). Consent from staff members was inferred as staff were freely able to collect a sample tube or given the choice to not participate at all. The research was carried out in according with the Declaration of Helsinki. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/30 CRDT- 2026/10/28 00:47 PHST- 2025/10/16 00:00 [received] PHST- 2026/07/13 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:47 [entrez] PHST- 2026/07/30 00:00 [pmc-release] AID - 10.1186/s13073-026-01725-8 [pii] AID - 1725 [pii] AID - 10.1186/s13073-026-01725-8 [doi] PST - epublish SO - Genome Med. 2026 Jul 30;18(1):166. doi: 10.1186/s13073-026-01725-8. PMID- 42947242 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2050-7283 (Electronic) IS - 2050-7283 (Linking) VI - 14 IP - 1 DP - 2026 Oct 22 TI - Patterns of attentional and recognition-memory processing of emotional information among Chinese middle school students with different exercise volumes. LID - 10.1186/s40359-026-05840-4 [doi] LID - 1554 AB - BACKGROUND: Physical exercise is closely associated with adolescent mental health, yet emotional information processing among adolescents with different exercise volumes remains insufficiently understood. This study examined attentional and recognition-memory processing of positive and negative emotional information among middle school students with different exercise volumes. METHODS: Using convenience sampling, 300 copies of a modified 3-month-recall version of the Physical Activity Rating Scale-3 (PARS-3) were distributed at a middle school, yielding 291 valid questionnaires. In a cross-sectional extreme-group design, 60 students (30 per group) were recruited from the extreme ends of the high- and low-exercise-volume categories to complete the behavioral experiment. Task 1 used a dot-probe task to examine reaction time, probe congruency, and vigilance and disengagement/avoidance indices in response to positive and negative emotional pictures. Task 2 used a learning-recognition task to examine recognition accuracy, recognition sensitivity (d'), and response criterion (c) for positive and negative faces. RESULTS: In the dot-probe task, the Group x Valence interaction was significant, and the low-exercise-volume group responded faster to negative than to positive pictures. The Group x Valence interaction for the attentional disengagement/avoidance index was also significant, with the low-exercise-volume group showing a larger positive-picture disengagement/avoidance index, a pattern consistent with greater difficulty disengaging attention from positive pictures. In the learning-recognition task, Group x Valence interactions were significant for both d' and recognition accuracy, whereas the corresponding interaction for c was not significant. The low-exercise-volume group showed lower d' for positive than for negative faces, and its d' for positive faces was lower than that of the high-exercise-volume group; the accuracy analysis showed a corresponding within-group valence difference. CONCLUSIONS: Middle school students with different exercise volumes showed different valence-related patterns of emotional information processing, primarily in overall reaction time, the derived disengagement/avoidance index, and recognition sensitivity. Overall, exercise volume was associated with emotional information processing in adolescents. Because the behavioral sample was recruited from the extreme ends of the screening distribution, the observed group differences and effect sizes characterize selected extreme-group contrasts; they are not population-level estimates for typical high- versus low-exercise-volume adolescents. Longitudinal and experimental studies are needed to examine causal relationships. CI - (c) 2026. The Author(s). FAU - Chen, Kaiyu AU - Chen K AD - Wuxi Vocational Institute of Commerce, Wuxi, China. AD - School of Education Science, Jiangsu Normal University, Xuzhou, China. FAU - Wang, Pengfei AU - Wang P AUID- ORCID: 0000-0003-2380-5541 AD - School of Education Science, Jiangsu Normal University, Xuzhou, China. wppsy1525@gmail.com. AD - School of Psychology, Nanjing Normal University, Nanjing, 210097, China. wppsy1525@gmail.com. LA - eng GR - 2024KJD010/Science and Technology Innovation Support Program for Young Scholars in Shandong Universities/ PT - Journal Article DEP - 20261022 PL - England TA - BMC Psychol JT - BMC psychology JID - 101627676 RN - Chinese people SB - IM MH - Humans MH - *Attention/physiology MH - *Exercise/psychology MH - Adolescent MH - Male MH - *Emotions/physiology MH - Female MH - *Recognition, Psychology/physiology MH - China MH - Students/psychology MH - Reaction Time MH - Cross-Sectional Studies MH - Child MH - East Asian People PMC - PMC13715273 OTO - NOTNLM OT - Attentional processing OT - Emotional information processing OT - Exercise volume OT - Learning-recognition OT - Middle school students COIS- Declarations. Ethics approval and consent to participate: All procedures were conducted in accordance with the Declaration of Helsinki and relevant ethical principles and institutional guidelines for research involving human participants. The study protocol was reviewed and approved by the Biomedical Research Ethics Committee of Nanjing Normal University (approval No. NNU202503051). All participants were minors. Before data collection, each student provided written assent to participate, and written informed consent was obtained from a parent or legal guardian. Students took part only after both the student assent and parental or legal-guardian consent procedures had been completed. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/22 CRDT- 2026/10/28 00:46 PHST- 2026/06/01 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:46 [entrez] PHST- 2026/10/22 00:00 [pmc-release] AID - 10.1186/s40359-026-05840-4 [pii] AID - 5840 [pii] AID - 10.1186/s40359-026-05840-4 [doi] PST - epublish SO - BMC Psychol. 2026 Oct 22;14(1):1554. doi: 10.1186/s40359-026-05840-4. PMID- 42947241 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2050-7283 (Electronic) IS - 2050-7283 (Linking) VI - 14 IP - 1 DP - 2026 Jul 31 TI - Assessing emotional intelligence levels among Lebanese medical students: exploring factors and implications for emotional well-being. LID - 10.1186/s40359-026-05243-5 [doi] LID - 1555 AB - BACKGROUND: Emotional intelligence (EI) plays an important role in medical students' academic and professional development, as it influences communication, empathy, and stress management. This study aimed to assess EI levels among Lebanese medical students enrolled in universities adopting the European teaching system and to identify factors associated with EI levels. METHODS: This cross-sectional study was conducted among Lebanese medical students from Beirut Arab University, Lebanese University, and Saint Joseph University. Data were collected using an online questionnaire distributed through a snowball sampling technique. The questionnaire included sociodemographic and lifestyle characteristics, the Schutte Self-Report Emotional Intelligence Test (SSEIT), and the Perceived Stress Scale (PSS). Statistical analyses were performed using SPSS, and a p-value < 0.05 was considered statistically significant. RESULTS: A total of 416 medical students were included in the final analysis. The mean EI score was 123.4 (SD = 17.76). Overall, 68.0% of participants had average EI scores, 16.3% had unusually high EI scores, and 15.6% had unusually low EI scores. Higher EI was significantly associated with lower perceived stress, happiness, dog ownership, alcohol consumption, academic year, governorate, and self-pride. In addition, participants who were motivated, happy, proud of themselves, and had friendships lasting more than four years reported lower stress levels. CONCLUSIONS: Most Lebanese medical students demonstrated average EI levels. Lower stress and positive psychosocial characteristics were associated with higher EI. These findings highlight the importance of promoting emotional well-being and stress management strategies during medical education to support students' personal and professional development. CI - (c) 2026. The Author(s). FAU - El Sayed, Hadi AU - El Sayed H AD - Faculty of Medicine, Beirut Arab University, Beirut, Lebanon. FAU - Yakan, Jaydaa AU - Yakan J AD - Faculty of Medicine, Beirut Arab University, Beirut, Lebanon. FAU - Shmeisani, Ali AU - Shmeisani A AD - Faculty of Medicine, Beirut Arab University, Beirut, Lebanon. FAU - Saadeh, Wassim AU - Saadeh W AD - Faculty of Medicine, Beirut Arab University, Beirut, Lebanon. FAU - Diab, Hana AU - Diab H AD - Faculty of Medicine, Beirut Arab University, Beirut, Lebanon. FAU - Dabboussi, Jana AU - Dabboussi J AD - Faculty of Medicine, Beirut Arab University, Beirut, Lebanon. FAU - El Ali, Aya AU - El Ali A AD - Faculty of Medicine, Beirut Arab University, Beirut, Lebanon. FAU - Chahoud, Michele AU - Chahoud M AD - Faculty of Medicine, Beirut Arab University, Beirut, Lebanon. m.chahoud@bau.edu.lb. LA - eng PT - Journal Article DEP - 20260731 PL - England TA - BMC Psychol JT - BMC psychology JID - 101627676 SB - IM MH - Humans MH - *Emotional Intelligence MH - *Students, Medical/psychology/statistics & numerical data MH - Lebanon MH - Female MH - Male MH - Cross-Sectional Studies MH - Adult MH - Young Adult MH - Psychological Well-Being MH - Stress, Psychological/psychology MH - Subjective Stress MH - Surveys and Questionnaires PMC - PMC13715191 OTO - NOTNLM OT - Emotional intelligence OT - Health OT - Medical students OT - Stress COIS- Declarations. Ethics approval and consent to participate: The study received ethical approval from the Institutional Review Board of Beirut Arab University (IRB No. 2024-H-0143-M-R-0585). Participation was voluntary, and electronic informed consent was obtained from all participants before accessing the questionnaire. Participants who selected "I do not consent" were unable to proceed with the survey. No personally identifiable information was collected, and all responses remained anonymous and confidential. All study procedures were conducted in accordance with the principles of the Declaration of Helsinki. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:46 PHST- 2026/05/13 00:00 [received] PHST- 2026/07/22 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:46 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s40359-026-05243-5 [pii] AID - 5243 [pii] AID - 10.1186/s40359-026-05243-5 [doi] PST - epublish SO - BMC Psychol. 2026 Jul 31;14(1):1555. doi: 10.1186/s40359-026-05243-5. PMID- 42947237 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 0778-7367 (Print) IS - 2049-3258 (Electronic) IS - 0778-7367 (Linking) VI - 84 IP - 1 DP - 2026 Jul 31 TI - Evaluating warning label designs for front-of-package nutrition policy: evidence from an explanatory mixed-methods study among young Chinese consumers. LID - 10.1186/s13690-026-02026-7 [doi] LID - 229 AB - BACKGROUND: Front-of-package (FoP) warning labels (WLs) are widely used internationally to guide healthier food and beverage choices. China has not yet implemented a standard FoP system, and evidence on how different WL designs influence young Chinese consumers remains limited. This study examined the effects of two WL formats and explored how consumers perceived and responded to these labels. METHODS: An explanatory mixed-methods design was used. A virtual online supermarket experiment assigned 159 participants to a Black-octagon WL group, a Black-shield WL group, or a No-FoP control group, and their beverage selections were recorded and analyzed. Semi-structured interviews were conducted with 13 participants to explore perceptions and responses to WLs, and thematic analysis was used. RESULTS: Both WLs reduced the selection of high-sugar beverages compared with the control group. The Black-shield WL produced healthier choices than the Black-octagon WL, reflected in lower nutritional quality scores, energy content, and sugar content in selected beverages. Interviews identified four themes explaining these effects: visual characteristics shaping attractiveness and healthfulness perception, influence on consumer behavior, overall evaluation of WLs, and suggestions for improving design and implementation. WLs captured attention, increased risk perception, and triggered emotional responses that shaped choice. The shield label was viewed as more credible and acceptable, while the octagon label evoked stronger threat and occasional resistance. CONCLUSION: Both FoP WLs effectively reduced high sugar beverage choices among young Chinese consumers, and the Black-shield label showed stronger behavioral impact than the Black-octagon label. Young consumers viewed the shield as more credible and acceptable, while the octagon evoked stronger perceived threat and occasional resistance. These findings highlight the health-guiding value of WLs and provide culturally relevant evidence to inform China's future FoP nutrition labeling policy. CI - (c) 2026. The Author(s). FAU - Guo, Zhiyi AU - Guo Z AUID- ORCID: 0009-0006-0836-1929 AD - School of the Arts, University Sains Malaysia, Penang, 11800, Malaysia. zhiyi@student.usm.my. FAU - Mustafa, Muhizam AU - Mustafa M AD - School of the Arts, University Sains Malaysia, Penang, 11800, Malaysia. mmuhizam@usm.my. FAU - Ning, Yueyue AU - Ning Y AD - School of the Arts, University Sains Malaysia, Penang, 11800, Malaysia. ningyueyue@student.usm.my. LA - eng PT - Journal Article DEP - 20260731 PL - England TA - Arch Public Health JT - Archives of public health = Archives belges de sante publique JID - 9208826 PMC - PMC13711967 OTO - NOTNLM OT - Consumer behavior OT - Front-of-package warning labels OT - Mixed-methods study OT - Sugar-sweetened beverages OT - Visual design features COIS- Declarations. Ethics approval and consent to participate: This study was approved by the Human Research Ethics Committee of Universiti Sains Malaysia (USM/JEPeM/PP/25020205). The study was conducted in accordance with the ethical principles of the Declaration of Helsinki. All participants were informed about the purpose and procedures of the study, assured of confidentiality, and provided written informed consent before participation. Participation was voluntary, and participants could withdraw at any time without consequence. Consent for publication: Not applicable. This study does not include any identifiable personal data or images. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:46 PHST- 2025/12/01 00:00 [received] PHST- 2026/07/25 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:46 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s13690-026-02026-7 [pii] AID - 2026 [pii] AID - 10.1186/s13690-026-02026-7 [doi] PST - epublish SO - Arch Public Health. 2026 Jul 31;84(1):229. doi: 10.1186/s13690-026-02026-7. PMID- 42947229 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2050-7283 (Electronic) IS - 2050-7283 (Linking) VI - 14 IP - 1 DP - 2026 Jul 31 TI - Health-promoting behaviors in perimenopausal women based on the COM-B model: a latent profile analysis. LID - 10.1186/s40359-026-05218-6 [doi] LID - 1557 AB - BACKGROUND: Perimenopause is a critical transitional stage in women's lives and is characterized by substantial physiological and psychological changes that may influence health behaviors and long-term health outcomes. Health-promoting behaviors play an important role in preventing chronic diseases and improving quality of life among perimenopausal women. However, these behaviors may vary considerably across individuals, and the psychosocial factors underlying different behavioral patterns remain unclear. Guided by the Capability-Opportunity-Motivation-Behaviors (COM-B) model, this study aimed to identify latent profiles of health-promoting behaviors among perimenopausal women and to examine their associated factors. METHODS: This cross-sectional study included 500 women aged 40-55 years who were recruited by convenience sampling from the gynecology outpatient clinic of Dongguan Maternal and Child Health Hospital, China. Perimenopausal status was determined by a gynecologist with reference to STRAW + 10 menstrual criteria, defined as a persistent difference of at least 7 days in consecutive cycle lengths or an amenorrhea interval of at least 60 days but less than 12 months. Health-promoting behavior was assessed using the revised Chinese Health-Promoting Lifestyle Profile II. Latent profile analysis was conducted using six dimension scores. Theory-informed ordinal logistic regression examined associations of eHealth literacy, Family APGAR score, general self-efficacy, and prespecified covariates with profile level; a sensitivity model additionally adjusted for self-rated health, sleep quality, and body mass index category. RESULTS: Three ordered profiles of health-promoting behavior were identified: low-level (32.8%), moderate-level (46.4%), and high-level (20.8%). Their broadly parallel patterns across the six HPLP-II-R dimensions indicated primarily graded differences in overall health-promoting behavior. Higher eHealth literacy (OR = 1.266, 95% CI: 1.198-1.338), Family APGAR scores (OR = 1.137, 95% CI: 1.041-1.242), and general self-efficacy (OR = 1.125, 95% CI: 1.081-1.170) were associated with greater odds of membership in a higher-level profile. CONCLUSIONS: Health-promoting behaviors among perimenopausal women show substantial heterogeneity. Higher eHealth literacy, Family APGAR scores, and general self-efficacy-reflecting selected aspects of psychological capability, social opportunity, and reflective motivation within the COM-B framework-were associated with greater odds of membership in a higher-level health-promoting behavior profile. These may provide reference evidence for the importance of developing targeted health promotion strategies to support perimenopausal women in adopting healthier lifestyles. CI - (c) 2026. The Author(s). FAU - Fu, Yayin AU - Fu Y AD - School of Nursing, Guangdong Medical University, Songshan Lake Science and Technology Industrial Park, 1 Xincheng Avenue, Dongguan, Guangdong, 523808, China. FAU - Jin, Lin AU - Jin L AD - School of Nursing, Guangdong Medical University, Songshan Lake Science and Technology Industrial Park, 1 Xincheng Avenue, Dongguan, Guangdong, 523808, China. FAU - Zhou, Qiaoxuan AU - Zhou Q AD - School of Nursing, Guangdong Medical University, Songshan Lake Science and Technology Industrial Park, 1 Xincheng Avenue, Dongguan, Guangdong, 523808, China. FAU - Yang, Xuemin AU - Yang X AD - Dongguan Maternal and Child Health Hospital, 99 Zhenxing Road, Dongcheng District, Dongguan, Guangdong, 523057, P.R. China. FAU - Liang, Wanxia AU - Liang W AD - Dongguan Maternal and Child Health Hospital, 99 Zhenxing Road, Dongcheng District, Dongguan, Guangdong, 523057, P.R. China. FAU - Cui, Zheng'ai AU - Cui Z AD - School of Humanities and Management, Guangdong Medical University, Songshan Lake Science and Technology Industrial Park, 1 Xincheng Avenue, Dongguan, Guangdong, 523808, China. FAU - Cui, Ying'ai AU - Cui Y AD - School of Nursing, Guangdong Medical University, Songshan Lake Science and Technology Industrial Park, 1 Xincheng Avenue, Dongguan, Guangdong, 523808, China. xiaocui@gdmu.edu.cn. FAU - Duan, Dongmei AU - Duan D AD - School of Nursing, Guangdong Medical University, Songshan Lake Science and Technology Industrial Park, 1 Xincheng Avenue, Dongguan, Guangdong, 523808, China. duandm76@163.com. AD - Dongguan Maternal and Child Health Hospital, 99 Zhenxing Road, Dongcheng District, Dongguan, Guangdong, 523057, P.R. China. duandm76@163.com. LA - eng GR - No. 2026QN12/This research was funded by the 2026 Dongguan Philosophy and Social Sciences Planning Youth Project/ PT - Journal Article DEP - 20260731 PL - England TA - BMC Psychol JT - BMC psychology JID - 101627676 SB - IM MH - Humans MH - Female MH - *Perimenopause/psychology MH - Middle Aged MH - Cross-Sectional Studies MH - Adult MH - *Health Behavior MH - Self Efficacy MH - China MH - *Motivation PMC - PMC13715284 OTO - NOTNLM OT - COM-B model OT - Health-promoting behaviors OT - Latent profile analysis OT - Perimenopause OT - Stratified nursing care COIS- Declarations. Ethics approval and consent to participate: This study was approved by the Medical Ethics Committee of Dongguan Maternal and Child Health Hospital (Approval No. 2025 - 143) and was conducted in accordance with the Declaration of Helsinki and relevant institutional and national guidelines and regulations. All participants were informed of the purpose and procedures of the study and provided written informed consent prior to participation. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:46 PHST- 2026/04/21 00:00 [received] PHST- 2026/07/16 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:46 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s40359-026-05218-6 [pii] AID - 5218 [pii] AID - 10.1186/s40359-026-05218-6 [doi] PST - epublish SO - BMC Psychol. 2026 Jul 31;14(1):1557. doi: 10.1186/s40359-026-05218-6. PMID- 42947226 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2052-1847 (Print) IS - 2052-1847 (Electronic) IS - 2052-1847 (Linking) VI - 18 IP - 1 DP - 2026 Jul 31 TI - Lower-limb coordination during the rear-leg roundhouse kick in competitive taekwondo: a vector-coding analysis. LID - 10.1186/s13102-026-01831-5 [doi] LID - 528 AB - OBJECTIVE: To investigate whether differences exist in coupling angle (CA)-derived joint coordination pattern characteristics and coordination variability between the dominant side (DS) and non-dominant side (NS) during successful scoring rear-leg roundhouse kick (RLRK) in competitive taekwondo athletes. METHODS: Twelve male taekwondo athletes were recruited to perform RLRK with both the DS and NS. Lower-limb kinematic data were collected using a Vicon three-dimensional motion capture system. Signals from the Daedo electronic protector and a Kistler force platform were used for valid trial selection and movement event identification. Custom-written MATLAB code was used to calculate CA trajectories and coordination variability for lower-limb joint couplings. CA data were analyzed using circular statistics, with mean CA used to characterize CA-derived joint coordination pattern characteristics, and coordination variability was compared using paired-samples t-tests. RESULTS: No significant bilateral differences in mean CA were observed during the knee-lift phase. The bilateral differences in mean CA were mainly observed during the kicking and recovery phases, reflecting side-related differences in coordination characteristics represented by CA. Specifically, significant bilateral differences were found in the frontal-plane hip-ankle coupling and sagittal-plane knee-ankle coupling during the kicking phase (p = 0.037; p = 0.026), and in the sagittal-plane knee-ankle coupling during the recovery phase (p = 0.016). Significant side-related differences in coordination variability were observed across all three movement phases. During the knee-lift and kicking phases, significant differences were found in the sagittal-plane knee-ankle coupling (p = 0.002; p = 0.015). During the recovery phase, significant bilateral differences were observed in the frontal-plane hip-ankle coupling (p = 0.008), sagittal-plane hip-knee coupling (p = 0.027), and sagittal-plane knee-ankle coupling (p = 0.044). CONCLUSIONS: Competitive taekwondo athletes exhibit side-specific lower-limb joint coordination control strategies when executing RLRK. These differences were reflected in mean CA-based coordination pattern characteristics and coordination variability. Lower coordination variability in certain joint couplings on the DS during the knee-lift and kicking phases suggests that the DS may adopt a relatively more stable coordination control strategy during the key output stages of the movement. In the recovery phase, bilateral differences were mainly reflected in joint-specific coordination organization and in the distribution of coordination variability. CI - (c) 2026. The Author(s). FAU - Guo, Qi AU - Guo Q AD - School of Intelligent Sports Engineering, Wuhan Sports University, No. 461 Luoyu Road, Wuhan, Hubei Province, 430079, China. AD - Key Laboratory of Sports Engineering of General Administration of Sports of China, Wuhan Sports University, Wuhan, 430079, China. AD - Engineering Research Center of Sports Health Intelligent Equipment of Hubei Province, Wuhan Sports University, Wuhan, 430079, China. FAU - Ma, Yong AU - Ma Y AD - School of Intelligent Sports Engineering, Wuhan Sports University, No. 461 Luoyu Road, Wuhan, Hubei Province, 430079, China. mayong@whsu.edu.cn. AD - Key Laboratory of Sports Engineering of General Administration of Sports of China, Wuhan Sports University, Wuhan, 430079, China. mayong@whsu.edu.cn. AD - Engineering Research Center of Sports Health Intelligent Equipment of Hubei Province, Wuhan Sports University, Wuhan, 430079, China. mayong@whsu.edu.cn. AD - Specialised Research Centre for High-Quality Development of Competitive Sports, Wuhan Sports University, Wuhan, 430079, China. mayong@whsu.edu.cn. FAU - Liu, Lin AU - Liu L AD - School of Education (Normal School), Dongguan University of Technology, Dongguan, 523808, China. AD - Sports and Health Industry Science and Technology Innovation Center, Dongguan University of Technology, Dongguan, 523808, China. FAU - Liu, Gan AU - Liu G AD - School of Intelligent Sports Engineering, Wuhan Sports University, No. 461 Luoyu Road, Wuhan, Hubei Province, 430079, China. AD - Key Laboratory of Sports Engineering of General Administration of Sports of China, Wuhan Sports University, Wuhan, 430079, China. AD - Engineering Research Center of Sports Health Intelligent Equipment of Hubei Province, Wuhan Sports University, Wuhan, 430079, China. FAU - Jia, Mengyao AU - Jia M AD - School of Intelligent Sports Engineering, Wuhan Sports University, No. 461 Luoyu Road, Wuhan, Hubei Province, 430079, China. jmy15737132785@163.com. AD - Key Laboratory of Sports Engineering of General Administration of Sports of China, Wuhan Sports University, Wuhan, 430079, China. jmy15737132785@163.com. AD - Engineering Research Center of Sports Health Intelligent Equipment of Hubei Province, Wuhan Sports University, Wuhan, 430079, China. jmy15737132785@163.com. LA - eng GR - 2021-05/14th Five Year-Plan Advantageous and Characteristic Disciplines (Groups) of Colleges and Universities in Hubei Province/ GR - 21KT02/East Lake Scholars Sponsorship Program of Wuhan Sports University in China, the Science and Technology Team Foundation of Wuhan Sports University/ PT - Journal Article DEP - 20260731 PL - England TA - BMC Sports Sci Med Rehabil JT - BMC sports science, medicine & rehabilitation JID - 101605016 PMC - PMC13715184 OTO - NOTNLM OT - Dominant side OT - Motor coordination OT - Non-dominant side OT - Rear-leg roundhouse kick OT - Vector coding COIS- Declarations. Ethics approval and consent to participate: The study involving human participants was reviewed and approved by the Ethics Committee of Wuhan Sports University. All procedures were conducted in accordance with the Declaration of Helsinki, as well as local legislation and institutional requirements. Written informed consent was obtained from all participants prior to participation (see Supplementary Material Document 1). Consent for publication: Not applicable. The manuscript does not contain any individual person's data, images, or videos in an identifiable form. Individual-level anthropometric and training-experience information has been removed from Table 1 to protect participant anonymity. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:46 PHST- 2026/04/22 00:00 [received] PHST- 2026/06/23 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:46 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s13102-026-01831-5 [pii] AID - 1831 [pii] AID - 10.1186/s13102-026-01831-5 [doi] PST - epublish SO - BMC Sports Sci Med Rehabil. 2026 Jul 31;18(1):528. doi: 10.1186/s13102-026-01831-5. PMID- 42947218 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1749-799X (Electronic) IS - 1749-799X (Linking) VI - 21 IP - 1 DP - 2026 Oct 6 TI - Flap reconstruction versus dermal regeneration template for finger soft-tissue defects: comparative clinical outcomes. LID - 10.1186/s13018-026-07280-7 [doi] LID - 614 AB - BACKGROUND: Finger soft-tissue defects require thin, pliable, and durable coverage that preserves tendon gliding and hand function. Flap reconstruction remains the standard approach, providing well-vascularized tissue and reliable single-stage coverage, but is associated with donor-site morbidity and technical complexity. These limitations have prompted the search for alternative reconstructive strategies. Dermal regeneration templates (DRTs) have emerged as a potential option; however, comparative data evaluating their functional performance in finger defects remain limited. This study aimed to compare DRT-based reconstruction with flap surgery using standardized clinical and functional outcomes. METHODS: A retrospective comparative study was conducted including 99 fingers from 89 patients with isolated finger soft-tissue defects treated between May 2020 and October 2022. Patients underwent either flap reconstruction (n = 47) or DRT-based reconstruction (n = 52). Functional and clinical outcomes, including total active motion (TAM), Quick Disabilities of the Arm, Shoulder and Hand (QuickDASH) score, key pinch strength (KPS), scar quality (Vancouver Scar Scale, VSS), operative time, and complications, were evaluated. Multivariable regression analyses were performed to assess the independent effect of treatment modality. Additional patient-level sensitivity and cluster-adjusted analyses were conducted to account for potential within-patient correlation. RESULTS: Flap reconstruction resulted in significantly higher TAM (210.0 +/- 26.0 degrees vs. 192.2 +/- 22.6 degrees , p < 0.001) and lower QuickDASH scores (12.64 +/- 8.41 vs. 21.77 +/- 8.41, p < 0.001) compared with DRT. These differences remained significant after adjustment for confounders and remained robust in patient-level sensitivity and cluster-adjusted analyses. DRT-based reconstruction was associated with significantly shorter operative time (25 (15-40) vs. 60 (40-80) min, p < 0.001). Additional split-thickness skin grafting was required more frequently in the DRT group than in the flap group (78.8% vs. 51.1%, p = 0.004). No significant differences were observed between groups in KPS or major complication rates, while VSS showed a non-significant trend toward better scar quality in the DRT group. Treatment modality was not a significant predictor of major complications. Defect size was not independently associated with clinical outcomes. CONCLUSIONS: Flap reconstruction provides superior functional outcomes in terms of joint motion and disability scores in finger soft-tissue defects, whereas the DRT product used in this cohort may represent a flap-harvest-sparing alternative with shorter operative time and favorable scar characteristics in selected cases. Treatment selection should be individualized based on functional demands and clinical priorities. LEVEL OF EVIDENCE: Level III, retrospective comparative study. CI - (c) 2026. The Author(s). FAU - Atilgan, Numan AU - Atilgan N AD - Department of Hand Surgery, Deva Hospital, Gaziantep, Turkey. FAU - Rizvanoglu, Ibrahim Halil AU - Rizvanoglu IH AD - Department of Orthopedics and Traumatology, NCR International Hospital, Mucahitler Neighborhood, No:56, 27090, Gaziantep, Sehitkamil, Turkey. dr.rizvanoglu@gmail.com. LA - eng PT - Comparative Study PT - Journal Article DEP - 20261006 PL - England TA - J Orthop Surg Res JT - Journal of orthopaedic surgery and research JID - 101265112 SB - IM MH - Humans MH - Female MH - Male MH - Retrospective Studies MH - *Surgical Flaps MH - *Soft Tissue Injuries/surgery MH - Treatment Outcome MH - *Finger Injuries/surgery/physiopathology MH - *Plastic Surgery Procedures/methods MH - Middle Aged MH - Adult MH - *Skin Transplantation/methods MH - Aged MH - Young Adult PMC - PMC13711807 OTO - NOTNLM OT - Dermal regeneration template OT - Finger injuries OT - Flap reconstruction OT - Scar quality OT - Soft tissue defects COIS- Declarations. Ethics approval and consent to participate: Ethical approval for this study was obtained from the Harran University Clinical Research Ethics Committee (HRU/22.13.06; July 4, 2022). The study was conducted in accordance with the principles of the Declaration of Helsinki. Consent for publication: Not applicable. Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/06 CRDT- 2026/10/28 00:45 PHST- 2026/05/28 00:00 [received] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:45 [entrez] PHST- 2026/10/06 00:00 [pmc-release] AID - 10.1186/s13018-026-07280-7 [pii] AID - 7280 [pii] AID - 10.1186/s13018-026-07280-7 [doi] PST - epublish SO - J Orthop Surg Res. 2026 Oct 6;21(1):614. doi: 10.1186/s13018-026-07280-7. PMID- 42947216 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1541-4337 (Electronic) IS - 1541-4337 (Linking) VI - 25 IP - 6 DP - 2026 Nov TI - Cereal Biomass Valorization for Functional Food Ingredients: Fractionation and Circular Biorefinery Strategies. LID - e70681 [pii] LID - 10.1111/1541-4337.70681 [doi] AB - Food processing must transition toward resource-efficient systems that operate within planetary boundaries. Cereals, as climate-resilient staples, offer strong potential to valorize nutrient-rich fractions. Zero-waste biorefinery concepts and green fractionation technologies, integrated with structure-function principles, enable the conversion of whole-grain biomass into functional ingredients for food applications. This review systematically evaluates recent advances in fractionation and separation technologies, including nonthermal physical and biochemical approaches. A Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA)-guided analysis of peer-reviewed articles (2020-2025) was conducted to assess extraction strategies, functional performance, and sustainability-related aspects. Mechanistic insights into recovered biopolymers are discussed to design process-functional relationships, with an emphasis on fractionation strategies such as synergistic recovery, fraction precision, and yield optimization relative to process complexity. The analysis shows that 46.75% of studies focused on common grains, whereas 53.25% utilized side streams (42.86% bran) for the recovery of proteins (32.47%), fibers (55.84%), lipids (10.39%), and phytochemicals (89.61%). Although hybrid techniques enhanced selectivity, multi-fraction recovery, and the preservation of biopolymer functionality, linear pathways dominated (70.13%), particularly in ultrasound-assisted methods (57.14%). Few studies investigated whole-system biorefinery approaches (14.29%), sustainability assessments (<5%), or pilot-scale validation (10.39%). Findings reveal critical opportunities for integrating life cycle assessment, techno-economic analysis, and digital modeling to facilitate scale-up and industrial translation. Furthermore, underutilized crops and industrial residues show promise for circular valorization. The review highlights the potential of sustainable biorefineries to reduce waste, enhance resource efficiency, and support circular-driven food innovation. CI - (c) 2026 The Author(s). Comprehensive Reviews in Food Science and Food Safety published by Wiley Periodicals LLC on behalf of Institute of Food Technologists. FAU - Biswas, Rahul AU - Biswas R AUID- ORCID: 0000-0002-2023-1936 AD - Department of Separation Science, LUT School of Engineering Sciences, Lappeenranta-Lahti University of Technology LUT, Kouvola, Finland. AD - Systemic Food Processing (SFP) Research Group, Kouvola Unit, Lappeenranta-Lahti University of Technology LUT, Kouvola, Finland. FAU - Poysa, Marjo AU - Poysa M AD - Department of Separation Science, LUT School of Engineering Sciences, Lappeenranta-Lahti University of Technology LUT, Kouvola, Finland. AD - Systemic Food Processing (SFP) Research Group, Kouvola Unit, Lappeenranta-Lahti University of Technology LUT, Kouvola, Finland. FAU - Oyeyinka, Samson Adeoye AU - Oyeyinka SA AD - Department of Separation Science, LUT School of Engineering Sciences, Lappeenranta-Lahti University of Technology LUT, Kouvola, Finland. AD - Centre of Excellence in Agri-Food Technologies, National Centre for Food Manufacturing, University of Lincoln, Holbeach, UK. AD - Centre for Innovative Food Research (CIFR), Innovation Research Group, Department of Biotechnology and Food Technology, Faculty of Science, University of Johannesburg, Johannesburg, Gauteng, South Africa. FAU - Demesa, Abayneh Getachew AU - Demesa AG AD - Department of Separation Science, LUT School of Engineering Sciences, Lappeenranta-Lahti University of Technology LUT, Kouvola, Finland. AD - Systemic Food Processing (SFP) Research Group, Kouvola Unit, Lappeenranta-Lahti University of Technology LUT, Kouvola, Finland. FAU - Wanninayake, Savindi AU - Wanninayake S AUID- ORCID: 0009-0009-4777-8155 AD - Department of Separation Science, LUT School of Engineering Sciences, Lappeenranta-Lahti University of Technology LUT, Kouvola, Finland. AD - Systemic Food Processing (SFP) Research Group, Kouvola Unit, Lappeenranta-Lahti University of Technology LUT, Kouvola, Finland. FAU - Perez-Vega, Samuel AU - Perez-Vega S AD - Department of Separation Science, LUT School of Engineering Sciences, Lappeenranta-Lahti University of Technology LUT, Kouvola, Finland. AD - Systemic Food Processing (SFP) Research Group, Kouvola Unit, Lappeenranta-Lahti University of Technology LUT, Kouvola, Finland. LA - eng GR - Regional Council of Kymenlaakso (Alueiden kestavan kasvun ja elinvoiman tukeminen, AKKE funding)/ PT - Journal Article PT - Review PL - United States TA - Compr Rev Food Sci Food Saf JT - Comprehensive reviews in food science and food safety JID - 101305205 RN - 0 (Food Ingredients) SB - IM MH - *Edible Grain/chemistry MH - *Biomass MH - *Food Handling/methods MH - *Functional Food/analysis MH - *Food Ingredients MH - Chemical Fractionation PMC - PMC13714167 OTO - NOTNLM OT - biopolymer recovery OT - cereal side streams OT - green separation technologies OT - structure-function relationships OT - zero-waste food systems COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/28 CRDT- 2026/10/28 00:45 PHST- 2026/09/21 00:00 [revised] PHST- 2026/04/23 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:45 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e70681 [pii] AID - CRF370681 [pii] AID - 10.1111/1541-4337.70681 [doi] PST - ppublish SO - Compr Rev Food Sci Food Saf. 2026 Nov;25(6):e70681. doi: 10.1111/1541-4337.70681. PMID- 42947205 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1754-1611 (Print) IS - 1754-1611 (Electronic) IS - 1754-1611 (Linking) VI - 20 IP - 1 DP - 2026 Jul 31 TI - aspA promoter as a tumor microenvironment-responsive autoswitch for anticancer agent expression in Escherichia coli. LID - 10.1186/s13036-026-00743-9 [doi] LID - 156 AB - Bacterial cancer therapy has emerged as a promising strategy for the local delivery of anticancer agents to solid tumors because bacteria preferentially colonize the tumor microenvironment (TME). However, strategies for exploiting metabolic features of the TME to achieve tumor-selective expression of anticancer agents remain limited. Here, we present a tumor-responsive gene expression system in Escherichia coli for tumor-selective expression of the recombinant immunotoxin psp-TGFa-PE38 (TP), exploiting its intrinsic ability to sense metabolic cues in the TME. To identify such TME-associated metabolic cues, we examined tumor metabolites in CT26 tumor-bearing mice and confirmed that tumors contained > 3-fold lower glucose and > 5-fold higher fumarate levels than those in the liver. In response to this nutrient heterogeneity, E. coli upregulated genes involved in fumarate respiration, including frdA, dctA, dcuB, and aspA, in the DcuSR regulon. aspA was most upregulated (65-fold) in tumors, which is indicative of the strongest activity of its promoter in the TME. We used the aspA promoter (P(aspA)) as a tumor-specific switch to control TP expression, targeting secreted functional TP and inducing cytotoxicity in vitro. TP was specifically expressed by P(aspA) in tumors, reducing the tumor size by 2.8-fold and extending survival by 35 days in vivo. Overall, these results suggest that the potential of P(aspA) as a tumor-specific expression system. CI - (c) 2026. The Author(s). FAU - Lee, Dogeun AU - Lee D AD - Division of Biomedical Convergence, College of Biomedical Science, Kangwon National University, Chuncheon, 24341, Republic of Korea. FAU - Hong, Seyeon AU - Hong S AD - Division of Biomedical Convergence, College of Biomedical Science, Kangwon National University, Chuncheon, 24341, Republic of Korea. FAU - Kim, Jihyeon AU - Kim J AD - Division of Biomedical Convergence, College of Biomedical Science, Kangwon National University, Chuncheon, 24341, Republic of Korea. FAU - Song, Miryoung AU - Song M AD - Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Yongin, 17035, Republic of Korea. songm@hufs.ac.kr. FAU - Lim, Daejin AU - Lim D AUID- ORCID: 0000-0003-0375-1857 AD - Division of Biomedical Convergence, College of Biomedical Science, Kangwon National University, Chuncheon, 24341, Republic of Korea. lim.d@kangwon.ac.kr. LA - eng GR - RS-2026-25487152/National Research Foundation of Korea/ GR - NRF-RS-2023-00210053/National Research Foundation of Korea/ GR - RS-2025-02263715/Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry/ GR - 2025-RISE-10-002/Regional Innovation System and Education program through the Gangwon RISE Center/ PT - Journal Article DEP - 20260731 PL - England TA - J Biol Eng JT - Journal of biological engineering JID - 101306640 PMC - PMC13715239 OTO - NOTNLM OT - E. coli OT - aspA promoter OT - Fumarate respiration OT - Nutrient heterogeneity OT - Tumor microenvironment OT - Tumor-specific expression COIS- Declarations. Ethics approval and consent to participate: All animal procedures were approved by the Institutional Animal Care and Use Committee of Kangwon National University (approval number KW-240326-3). Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:45 PHST- 2026/04/24 00:00 [received] PHST- 2026/07/26 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:45 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s13036-026-00743-9 [pii] AID - 743 [pii] AID - 10.1186/s13036-026-00743-9 [doi] PST - epublish SO - J Biol Eng. 2026 Jul 31;20(1):156. doi: 10.1186/s13036-026-00743-9. PMID- 42947197 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2072-1315 (Electronic) IS - 1606-0997 (Print) IS - 1606-0997 (Linking) VI - 45 IP - 1 DP - 2026 Jul 28 TI - Pathways linking to behavioural and biological risk factors on diabetes and overweight/obese among adults in urban slums from Western India: a structural equation modelling analysis. LID - 10.1186/s41043-026-01411-w [doi] LID - 237 AB - BACKGROUND: The prevalence of non-communicable diseases (NCDs) is on the rise in India, exhibiting significant regional disparities. This study aimed to evaluate sociodemographic, behavioural, biological and anthropometric risk factors associated with diabetes and overweight/obese among adults residing in urban slums of western India. METHODS: This cross-sectional community-based study in India involved 456 adults from urban slums, selected through three-stage sampling. Data collection was done by using the WHO STEPS approach, with participants providing informed consent and completing a pretested questionnaire covering sociodemographic, behavioral, and biological risk factors. They were interviewed, and anthropometric measurements were done using standardized procedures and fasting blood samples were analysed for biochemical parameters using standard laboratory methods. Results were expressed as proportions and analysis was done by using multivariate logistic regression, one-way ANOVA, & post hoc Bonferroni test. Structural equation modelling (SEM) was applied to evaluate direct and indirect associations involving Diabetes and BMI outcome- overweight/obese. RESULTS: The prevalence of Diabetes, hypertension and overweight/obese was 13.2%, 14.9%, and 28.1%, respectively. Behavioural risk factors included alcohol consumption (13.8%), smoking (23.2%), inadequate fruit consumption (51.3%), inadequate vegetable consumption (98.9%), and insufficient physical activity (78.7%). Biological risk factors included hypercholesterolaemia (19.5%), hypertriglyceridaemia (21.3%), and hyperhomocysteinaemia (69.5%). In multivariable logistic regression, age > 40 years (AOR 3.57, 95% CI: 1.60-7.96) was independently associated with Diabetes, while participants in BJ Prasad socioeconomic Class III had lower odds of Diabetes (AOR 0.24, 95% CI: 0.08-0.73). Smoking (AOR 3.22, 95% CI: 1.62-6.38), alcohol consumption (AOR 2.24, 95% CI: 1.03-4.89), and hyperhomocysteinaemia (AOR 2.35, 95% CI: 1.15-4.82) were independently associated with hypertension, whereas fruit consumption was associated with lower odds (AOR 0.37, 95% CI: 0.20-0.69). Insufficient physical activity was independently associated with overweight/obese (AOR 1.87, 95% CI: 1.11-3.15). The SEM demonstrated excellent model fit and identified significant associations of age (beta = 0.168, p < 0.001), smoking (beta = 0.143, p < 0.001), alcohol consumption (beta = 0.108, p < 0.01), hypercholesterolemia (beta = 0.097, p < 0.05), and socioeconomic status (beta = 0.092, p < 0.05) with Diabetes, while physical inactivity showed a significant direct association with overweight/obese (beta = 0.105, p < 0.05) and LDL cholesterol (beta = +0.121, p = 0.009) were significantly and positively associated with BMI overweight/obese. CONCLUSION: The findings highlight the importance of strengthening NCD surveillance and preventive health programmes in urban slum populations. CI - (c) 2026. The Author(s). FAU - Mahajan, Sudhanshu AU - Mahajan S AD - Department of Community Medicine, Bharati Vidyapeeth (Deemed to be University) Medical College, Pune, India. FAU - Patil, Sanjivani AU - Patil S AD - Department of Community Medicine, Bharati Vidyapeeth (Deemed to be University) Medical College, Pune, India. FAU - Deshmukh, Rupeshkumar AU - Deshmukh R AUID- ORCID: 0000-0002-9684-2098 AD - Department of Community Medicine, Bharati Vidyapeeth (Deemed to be University) Medical College, Pune, India. rupeshkumar.deshmukh@bharatividyapeeth.edu. FAU - Gawade Maindad, Gayatri AU - Gawade Maindad G AD - Department of Biochemistry, Bharati Vidyapeeth (Deemed to be University) Medical College, Pune, India. FAU - Gothankar, Jayashree AU - Gothankar J AD - Department of Community Medicine, Bharati Vidyapeeth (Deemed to be University) Medical College, Pune, India. FAU - Padwal, Meghana AU - Padwal M AD - Department of Biochemistry, Bharati Vidyapeeth (Deemed to be University) Medical College, Pune, India. FAU - Modak, Meera AU - Modak M AD - Department of Microbiology, Bharati Vidyapeeth (Deemed to be University) Medical College, Pune, India. FAU - Doke, Prakash AU - Doke P AD - Department of Community Medicine, Bharati Vidyapeeth (Deemed to be University) Medical College, Pune, India. LA - eng PT - Journal Article DEP - 20260728 PL - Bangladesh TA - J Health Popul Nutr JT - Journal of health, population, and nutrition JID - 100959228 SB - IM MH - Humans MH - India/epidemiology MH - Risk Factors MH - Male MH - Female MH - Cross-Sectional Studies MH - Adult MH - *Poverty Areas MH - Middle Aged MH - *Overweight/epidemiology/etiology MH - *Obesity/epidemiology/etiology MH - Latent Class Analysis MH - Urban Population/statistics & numerical data MH - Prevalence MH - *Diabetes Mellitus/epidemiology/etiology MH - Alcohol Drinking MH - Body Mass Index MH - *Health Behavior MH - Hypertension/epidemiology MH - Young Adult MH - Smoking MH - Socioeconomic Factors PMC - PMC13715145 OTO - NOTNLM OT - Diabetes OT - India OT - Overweight/obese OT - STEPS survey OT - Structural equation modelling COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/28 CRDT- 2026/10/28 00:44 PHST- 2026/01/19 00:00 [received] PHST- 2026/07/11 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:44 [entrez] PHST- 2026/07/28 00:00 [pmc-release] AID - 10.1186/s41043-026-01411-w [pii] AID - 1411 [pii] AID - 10.1186/s41043-026-01411-w [doi] PST - epublish SO - J Health Popul Nutr. 2026 Jul 28;45(1):237. doi: 10.1186/s41043-026-01411-w. PMID- 42947190 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1745-6150 (Electronic) IS - 1745-6150 (Linking) VI - 21 IP - 1 DP - 2026 Jul 30 TI - The RNA-binding protein KHSRP drives prostate cancer progression through a DUSP6 dependent axis. LID - 10.1186/s13062-026-00894-1 [doi] LID - 216 AB - BACKGROUND: Prostate cancer (PCa) remains a major clinical challenge, particularly because of its progression to more aggressive disease states, and the molecular mechanisms driving its progression are still incompletely understood. KH-type splicing regulatory protein (KHSRP) is a multifunctional RNA-binding protein involved in post-transcriptional gene regulation, but its role and downstream regulatory mechanisms in PCa remain unclear. METHODS: The clinical relevance of KHSRP in PCa was assessed using public datasets and patient specimens. We assessed the biological functions of KHSRP in vitro and in vivo, identified DUSP6 by integrative RNA-seq and RIP-seq analyses, and further validated the associated signaling and phenotypic changes using Western blotting and functional assays. RESULTS: KHSRP was significantly upregulated in PCa and was associated with aggressive clinicopathological features and poor prognosis. Functional experiments showed that KHSRP overexpression promoted proliferation, migration, and invasion while suppressing apoptosis in PCa cells, whereas KHSRP knockdown produced the opposite effects. Mechanistically, integrative RNA-seq and RIP-seq analyses identified DUSP6 as a candidate downstream target of KHSRP. Further validation using RNA immunoprecipitation assays and RNA decay assays demonstrated that KHSRP accelerated DUSP6 mRNA decay and repressed DUSP6 expression at the post-transcriptional level. Reduced DUSP6 expression was accompanied by enhanced ERK phosphorylation and coordinated activation of PI3K/AKT/mTOR signaling. Restoration of DUSP6 attenuated the pro-tumorigenic effects of KHSRP and weakened the associated signaling activation. Xenograft assays further confirmed the tumor-promoting role of KHSRP in vivo. CONCLUSION: KHSRP drives PCa progression through a DUSP6-dependent signaling axis by repressing DUSP6 at the post-transcriptional level and facilitating coordinated activation of ERK and PI3K/AKT/mTOR signaling. These findings identify a previously unrecognized KHSRP-DUSP6 regulatory axis in PCa and suggest that KHSRP may serve as a potential therapeutic target. CI - (c) 2026. The Author(s). FAU - Wang, Wenyun AU - Wang W AD - Department of Urology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, Gansu, 730000, China. AD - Gansu Province Clinical Research Center for Urinary System Disease, Lanzhou, Gansu, 730030, China. FAU - Zhao, Kun AU - Zhao K AD - Department of Urology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, Gansu, 730000, China. AD - Gansu Province Clinical Research Center for Urinary System Disease, Lanzhou, Gansu, 730030, China. FAU - Zhao, Yu AU - Zhao Y AD - Department of Medicine, Northwest Minzu University, Lanzhou, PR China. FAU - You, Chengyu AU - You C AD - Department of Urology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, Gansu, 730000, China. AD - Gansu Province Clinical Research Center for Urinary System Disease, Lanzhou, Gansu, 730030, China. FAU - Wang, Yanan AU - Wang Y AD - Department of Urology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, Gansu, 730000, China. AD - Gansu Province Clinical Research Center for Urinary System Disease, Lanzhou, Gansu, 730030, China. FAU - Zhou, Zhongze AU - Zhou Z AD - Department of Urology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, Gansu, 730000, China. AD - Gansu Province Clinical Research Center for Urinary System Disease, Lanzhou, Gansu, 730030, China. FAU - Fang, Qixiang AU - Fang Q AD - Department of Urology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, Gansu, 730000, China. AD - Gansu Province Clinical Research Center for Urinary System Disease, Lanzhou, Gansu, 730030, China. FAU - Xiao, Xi AU - Xiao X AD - Department of Urology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, Gansu, 730000, China. AD - Gansu Province Clinical Research Center for Urinary System Disease, Lanzhou, Gansu, 730030, China. FAU - Dong, Zhilong AU - Dong Z AUID- ORCID: 0000-0002-4919-8164 AD - Department of Urology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, Gansu, 730000, China. dzl19780829@163.com. AD - Gansu Province Clinical Research Center for Urinary System Disease, Lanzhou, Gansu, 730030, China. dzl19780829@163.com. LA - eng GR - Grant Number 23JRRA1631/Gansu Provincial Natural Science Foundation/ GR - Grant Number 2022-3-68/Lanzhou Science and Technology Plan Project/ GR - Grant Number 2025-NQ-053/Lanzhou Science and Technology Plan Project/ GR - Grant Number CY2023-MS-B14/Cuiying Scientific and Technological Innovation Program/ GR - Grant No. 31920260145/Fundamental Research Funds for the Central Universities of Northwest Minzu University/ PT - Journal Article DEP - 20260730 PL - England TA - Biol Direct JT - Biology direct JID - 101258412 RN - EC 3.1.3.48 (Dual Specificity Phosphatase 6) RN - 0 (RNA-Binding Proteins) RN - EC 3.1.3.48 (DUSP6 protein, human) RN - 0 (KHSRP protein, human) RN - 0 (Trans-Activators) SB - IM MH - Male MH - *Prostatic Neoplasms/genetics/metabolism/pathology MH - Humans MH - *Dual Specificity Phosphatase 6/metabolism/genetics MH - *RNA-Binding Proteins/metabolism/genetics MH - Animals MH - Disease Progression MH - Mice MH - Cell Line, Tumor MH - Gene Expression Regulation, Neoplastic MH - Cell Proliferation MH - Signal Transduction MH - Cell Movement MH - Trans-Activators PMC - PMC13715121 OTO - NOTNLM OT - DUSP6 OT - KHSRP OT - Prostate cancer OT - RNA binding protein COIS- Declarations. Ethics approval and consent to participate: This study was approved by the Ethics Committee of the Second Hospital of Lanzhou University for both human and animal experiments. Human tissue collection and related experiments were approved under Approval No. 2025 A-546. Written informed consent was obtained from all participants prior to sample collection. Animal experiments were conducted following institutional guidelines and were approved under Approval No. D2025-409. Consent for publication: All patients involved in this study provided written informed consent for the use of their clinical data for research and publication purposes. The consent process included disclosure that their de-identified data may be made publicly available online and in print following publication. No identifying information (such as name, initials, date of birth, or hospital number) has been included in this manuscript. The study protocol and consent procedures were reviewed and approved by the institutional ethics committee, and all efforts were made to ensure patient confidentiality and compliance with data protection regulations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/30 CRDT- 2026/10/28 00:44 PHST- 2026/04/29 00:00 [received] PHST- 2026/07/09 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:44 [entrez] PHST- 2026/07/30 00:00 [pmc-release] AID - 10.1186/s13062-026-00894-1 [pii] AID - 894 [pii] AID - 10.1186/s13062-026-00894-1 [doi] PST - epublish SO - Biol Direct. 2026 Jul 30;21(1):216. doi: 10.1186/s13062-026-00894-1. PMID- 42947178 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1476-4598 (Electronic) IS - 1476-4598 (Linking) VI - 25 IP - 1 DP - 2026 Jul 31 TI - Targeting circular RNAs to overcome therapeutic resistance in liver cancer: from mechanisms to clinical prospects. LID - 10.1186/s12943-026-02761-z [doi] LID - 257 AB - Primary liver cancer, including hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (iCCA), remains a leading cause of cancer-related death worldwide. Therapeutic resistance substantially limits the efficacy of chemotherapy, targeted therapy, immunotherapy, and radiotherapy. For example, only approximately 30% of patients with HCC derive clinical benefit from sorafenib, and acquired resistance commonly develops within six months, underscoring the urgent need to clarify the molecular basis of treatment failure. Circular RNAs (circRNAs) are covalently closed RNA molecules with high structural stability and tissue-specific expression. In liver cancer, circRNAs regulate therapeutic resistance through multiple mechanisms that converge on ferroptosis suppression, apoptosis evasion, DNA damage repair, oncogenic signaling, immune escape, and cancer stemness. Beyond the canonical competing endogenous RNA model, this review highlights emerging regulatory mechanisms involving RNA-binding protein interactions, circRNA-encoded functional micropeptides, and circRNA-mediated phase separation. Exosomal circRNAs also facilitate the intercellular transmission of resistant phenotypes and remodel the tumor immune microenvironment. We systematically summarize circRNA-mediated resistance across different therapeutic modalities and critically evaluate the available evidence according to experimental models, in vivo validation, and patient validation. Although circRNAs show potential as biomarkers for predicting therapeutic response and as targets for overcoming resistance, most candidates remain at the preclinical stage. Their clinical translation is constrained by the lack of standardized detection methods, insufficient multicenter validation, and inefficient and inadequately targeted in vivo delivery systems. Because most direct mechanistic evidence is derived from HCC, selected iCCA-related findings are incorporated where available. CI - (c) 2026. The Author(s). FAU - Zhou, Xinyi AU - Zhou X AD - Department of Liver Surgery, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, China. AD - Laboratory of Liver Surgery, West China Hospital, Sichuan University, Chengdu, China. FAU - Yang, Chenyu AU - Yang C AD - Department of Liver Surgery, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, China. AD - Laboratory of Liver Surgery, West China Hospital, Sichuan University, Chengdu, China. FAU - Lu, Jiong AU - Lu J AD - Division of Biliary Surgery, Department of General Surgery, West China Hospital, Sichuan University, Chengdu, China. AD - Research Center for Biliary Diseases, West China Hospital, Sichuan University, Chengdu, China. FAU - Huang, Tengda AU - Huang T AD - Department of Liver Surgery, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, China. AD - Laboratory of Liver Surgery, West China Hospital, Sichuan University, Chengdu, China. FAU - Ren, Shiqi AU - Ren S AD - Department of Liver Surgery, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, China. AD - Laboratory of Liver Surgery, West China Hospital, Sichuan University, Chengdu, China. FAU - Pan, Hongyuan AU - Pan H AD - Department of Liver Surgery, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, China. AD - Laboratory of Liver Surgery, West China Hospital, Sichuan University, Chengdu, China. FAU - Du, Ao AU - Du A AD - Department of Liver Surgery, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, China. AD - Laboratory of Liver Surgery, West China Hospital, Sichuan University, Chengdu, China. FAU - Zhou, Zhixiang AU - Zhou Z AD - Department of Liver Surgery, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, China. AD - Laboratory of Liver Surgery, West China Hospital, Sichuan University, Chengdu, China. FAU - Yuan, Kefei AU - Yuan K AD - Department of Liver Surgery, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, China. ykf13@163.com. AD - Laboratory of Liver Surgery, West China Hospital, Sichuan University, Chengdu, China. ykf13@163.com. LA - eng GR - 82573041/National Natural Science Foundation of China/ GR - 82373400/National Natural Science Foundation of China/ PT - Journal Article PT - Review DEP - 20260731 PL - England TA - Mol Cancer JT - Molecular cancer JID - 101147698 RN - 0 (RNA, Circular) RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - *RNA, Circular/genetics MH - *Liver Neoplasms/genetics/drug therapy/pathology/metabolism/therapy MH - *Drug Resistance, Neoplasm/genetics MH - Animals MH - Biomarkers, Tumor/genetics MH - Gene Expression Regulation, Neoplastic/drug effects MH - Carcinoma, Hepatocellular/genetics/drug therapy MH - Molecular Targeted Therapy PMC - PMC13715258 OTO - NOTNLM OT - Biomarker OT - Circular RNA OT - Immunotherapy OT - Liver Cancer OT - Targeted Therapy OT - Therapeutic Resistance COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:43 PHST- 2026/06/11 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:43 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s12943-026-02761-z [pii] AID - 2761 [pii] AID - 10.1186/s12943-026-02761-z [doi] PST - epublish SO - Mol Cancer. 2026 Jul 31;25(1):257. doi: 10.1186/s12943-026-02761-z. PMID- 42947177 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2731-3654 (Electronic) IS - 2731-3654 (Linking) VI - 19 IP - 1 DP - 2026 Jul 31 TI - Improving silage fermentation of distilled grain waste-based spent mushroom substrate using indigenous lactic acid bacteria: metagenomic insights. LID - 10.1186/s13068-026-02803-0 [doi] LID - 87 AB - BACKGROUND: Rising global mushroom production calls for recycling spent mushroom substrate (SMS). However, its high moisture content, susceptibility to spoilage, and low abundance of indigenous lactic acid bacteria (LAB) limit its use as silage. In this study, an effective silage inoculation strategy was developed to transform SMS derived from composted distilled grain waste (DGW) and colonized by Pleurotus ostreatus. RESULTS: Three indigenous lactic acid bacteria, including Limosilactobacillus fermentum L2, Weissella paramesenteroides W1, and Pediococcus acidilactici P1, were isolated and selected for their rapid growth and acidification capacity, and applied individually or in combination at different stages of fungal colonization. Compared with a commercial inoculant, all indigenous lactic acid bacteria (LAB) treatments, especially a combination of L. fermentum L2 and P. acidilactici P1, significantly enhanced silage fermentation, as evidenced by lower pH (4.04-4.73), 31-fold and 1.87-fold increases in lactic acid and acetic acid production, respectively, improved crude protein retention, and preserved dry matter contents. KEGG enrichment analysis demonstrated that carbon metabolic flux was markedly redirected toward lactic acid fermentation, while pathways related to protein degradation and biosafety were significantly suppressed in LAB-treated SMS silage. Weighted metabolic analysis revealed that LAB contributed most to fermentative processes, thereby enriching Pediococcus, Weissella, and Lactobacillus, whereas the contributions of non-LAB and potentially harmful microorganisms were substantially reduced. CONCLUSION: These results indicate that indigenous LAB enhance the ensiling efficiency of SMS by driving functional reallocation and pathway-level dominance, thereby improving fermentation efficiency, stability, and nutritional quality, highlighting their potential for promoting SMS silage as a high-quality ruminant feed resource. CI - (c) 2026. The Author(s). FAU - Zhang, Xinlei AU - Zhang X AD - School of Life Sciences and Medical Engineering, Anhui University, Hefei, 230601, Anhui, China. AD - Anhui Key Laboratory of Biocatalysis and Modern Biomanufacturing, Hefei, 230601, Anhui, China. AD - Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China. FAU - Yin, Diao AU - Yin D AD - School of Life Sciences and Medical Engineering, Anhui University, Hefei, 230601, Anhui, China. AD - Anhui Key Laboratory of Biocatalysis and Modern Biomanufacturing, Hefei, 230601, Anhui, China. AD - Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China. FAU - Liu, Pengyu AU - Liu P AD - School of Life Sciences and Medical Engineering, Anhui University, Hefei, 230601, Anhui, China. AD - Anhui Key Laboratory of Biocatalysis and Modern Biomanufacturing, Hefei, 230601, Anhui, China. AD - Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China. FAU - Song, Jialun AU - Song J AD - School of Life Sciences and Medical Engineering, Anhui University, Hefei, 230601, Anhui, China. FAU - Fang, Zemin AU - Fang Z AUID- ORCID: 0000-0002-9853-5470 AD - School of Life Sciences and Medical Engineering, Anhui University, Hefei, 230601, Anhui, China. zemin_fang@ahu.edu.cn. AD - Anhui Key Laboratory of Biocatalysis and Modern Biomanufacturing, Hefei, 230601, Anhui, China. zemin_fang@ahu.edu.cn. AD - Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China. zemin_fang@ahu.edu.cn. FAU - Liu, Juanjuan AU - Liu J AD - School of Life Sciences and Medical Engineering, Anhui University, Hefei, 230601, Anhui, China. liu_juan825@ahu.edu.cn. AD - Anhui Key Laboratory of Biocatalysis and Modern Biomanufacturing, Hefei, 230601, Anhui, China. liu_juan825@ahu.edu.cn. AD - Anhui Provincial Engineering Technology Research Center of Microorganisms and Biocatalysis, Hefei, 230601, Anhui, China. liu_juan825@ahu.edu.cn. LA - eng GR - 32370133/National Natural Science Foundation of China/ GR - 32470122/National Natural Science Foundation of China/ GR - 2508085Y011/Natural Science Foundation of Anhui Province/ PT - Journal Article DEP - 20260731 PL - England TA - Biotechnol Biofuels Bioprod JT - Biotechnology for biofuels and bioproducts JID - 9918300888906676 PMC - PMC13715123 OTO - NOTNLM OT - Animal feed OT - Lactic acid bacteria OT - Metagenomic analysis OT - Silage quality OT - Spent mushroom substrate COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publications: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:43 PHST- 2026/01/29 00:00 [received] PHST- 2026/07/23 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:43 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s13068-026-02803-0 [pii] AID - 2803 [pii] AID - 10.1186/s13068-026-02803-0 [doi] PST - epublish SO - Biotechnol Biofuels Bioprod. 2026 Jul 31;19(1):87. doi: 10.1186/s13068-026-02803-0. PMID- 42947174 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1746-6148 (Electronic) IS - 1746-6148 (Linking) VI - 22 IP - 1 DP - 2026 Jul 31 TI - Metabolic biomarkers and milk production indicators associated with elevated non-esterified fatty acids in holstein dairy cows. LID - 10.1186/s12917-026-05789-x [doi] LID - 638 AB - BACKGROUND: Negative energy balance (NEB) is a common metabolic challenge during early lactation in dairy cows and is characterized by increased mobilization of body fat reserves and elevated circulating non-esterified fatty acids (NEFA). This observational study aimed to evaluate associations between serum NEFA concentration, milk production and composition traits, and blood biochemical indicators in early lactation Holstein dairy cows, and to assess the diagnostic performance of selected variables associated with elevated NEFA. A total of 182 multiparous Holstein cows between 4 and 30 days in milk were enrolled. Blood samples and milk production data were collected once from each cow on the same day during early lactation. Based on serum NEFA concentration, cows were classified into low-NEFA ( 0.56 mmol/L; n = 36) groups. RESULTS: Cows with elevated NEFA concentrations showed significantly higher milk fat, fat-to-protein ratio (FPR), aspartate aminotransferase activity (AST), creatinine (CREA), sodium (Na), and chloride (Cl) concentrations, whereas magnesium (Mg), total protein (TP), urea, milk electrical conductivity, milk temperature, and gamma-glutamyl transferase (GGT) activity were lower (p < 0.05). Spearman correlation analysis demonstrated positive associations between NEFA and milk fat, FPR, AST, CREA, and Na concentrations, and negative associations with Mg, urea, milk temperature, Ca, phosphorus (P), and GGT activity. Receiver operating characteristic (ROC) analysis indicated that milk fat, CREA, Na, FPR, Mg, and AST showed the greatest ability to discriminate cows with elevated NEFA concentrations. CONCLUSIONS: Elevated NEFA concentrations in early lactation dairy cows were associated with changes in milk composition, liver enzyme activity, electrolyte balance, and mineral metabolism. The combination of milk parameters obtained from automatic milking systems and selected blood biochemical indicators may improve identification of cows experiencing negative energy balance under commercial farm conditions. CI - (c) 2026. The Author(s). FAU - Grige, Samanta AU - Grige S AD - Large Animal Clinic, Veterinary Academy, Lithuania University of Health Sciences, Tilzes Str. 18, Kaunas, LT-47181, Lithuania. samanta.grige1@lsmu.lt. FAU - Girdauskaite, Akvile AU - Girdauskaite A AD - Large Animal Clinic, Veterinary Academy, Lithuania University of Health Sciences, Tilzes Str. 18, Kaunas, LT-47181, Lithuania. FAU - Sabeckiene, Inga AU - Sabeckiene I AD - Large Animal Clinic, Veterinary Academy, Lithuania University of Health Sciences, Tilzes Str. 18, Kaunas, LT-47181, Lithuania. FAU - Lemboviciute, Gabija AU - Lemboviciute G AD - Large Animal Clinic, Veterinary Academy, Lithuania University of Health Sciences, Tilzes Str. 18, Kaunas, LT-47181, Lithuania. FAU - Sertvytyte, Greta AU - Sertvytyte G AD - Large Animal Clinic, Veterinary Academy, Lithuania University of Health Sciences, Tilzes Str. 18, Kaunas, LT-47181, Lithuania. FAU - Dzermeikaite, Karina AU - Dzermeikaite K AD - Large Animal Clinic, Veterinary Academy, Lithuania University of Health Sciences, Tilzes Str. 18, Kaunas, LT-47181, Lithuania. FAU - Kristolaityte, Justina AU - Kristolaityte J AD - Large Animal Clinic, Veterinary Academy, Lithuania University of Health Sciences, Tilzes Str. 18, Kaunas, LT-47181, Lithuania. FAU - Antanaitis, Ramunas AU - Antanaitis R AD - Large Animal Clinic, Veterinary Academy, Lithuania University of Health Sciences, Tilzes Str. 18, Kaunas, LT-47181, Lithuania. LA - eng PT - Journal Article DEP - 20260731 PL - England TA - BMC Vet Res JT - BMC veterinary research JID - 101249759 RN - 0 (Fatty Acids, Nonesterified) RN - 0 (Biomarkers) SB - IM MH - Animals MH - *Fatty Acids, Nonesterified/blood MH - Cattle/blood/physiology/metabolism MH - Female MH - *Lactation/physiology MH - Biomarkers/blood MH - *Milk/chemistry/metabolism PMC - PMC13715194 OTO - NOTNLM OT - Biosensors OT - Blood parameters OT - Dairy cattle OT - Early lactation OT - Innovations COIS- Declaration. Ethics approval and consent to participate: "All animal procedures were reviewed and approved by the Institutional Animal Care and Use Committee of the Lithuanian University of Health Sciences (Protocol No. G2-227, approved on 7 March 2025)". The animals included in this study were privately owned, and informed consent was obtained from the farm owner prior to the commencement of the study. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:43 PHST- 2026/04/13 00:00 [received] PHST- 2026/07/29 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:43 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s12917-026-05789-x [pii] AID - 5789 [pii] AID - 10.1186/s12917-026-05789-x [doi] PST - epublish SO - BMC Vet Res. 2026 Jul 31;22(1):638. doi: 10.1186/s12917-026-05789-x. PMID- 42947170 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1472-6947 (Electronic) IS - 1472-6947 (Linking) VI - 26 IP - 1 DP - 2026 Aug 1 TI - Rethinking hyperparameter optimization for efficient and explainable machine learning in civic health decision-making: empirical evidence from malaria severity prediction. LID - 10.1186/s12911-026-03732-6 [doi] LID - 440 AB - BACKGROUND: Malaria remains one of the most persistent infectious diseases worldwide, with hundreds of millions of cases annually. Machine learning has emerged as a promising tool for supporting clinical decision-making in malaria care, but the practical deployment of such models is constrained by hyperparameter optimization complexity. This study provides a comprehensive comparison of stochastic and deterministic hyperparameter optimization strategies for supervised machine learning models applied to the binary classification of malaria severity (severe vs. not-severe disease) among symptomatic patients. METHODS: We evaluated five optimization strategies (Grid Search, Random Search, Bayesian Optimization, Genetic Algorithms, and Hyperband Racing) across six supervised learning algorithms (Random Forest, Neural Networks, Support Vector Machines, Logistic Regression, XGBoost, and K-Nearest Neighbors). The classification task was binary severity prediction (Severe vs. Not-Severe) among symptomatic malaria patients. Models were trained on synthetic datasets with varying complexity and a real clinical malaria dataset comprising 337 patients with 16 clinical features (34.4% severe cases). Performance was assessed using 10-fold cross-validation repeated five times, with evaluation metrics including F1-score, accuracy, ROC AUC, Matthews Correlation Coefficient, and balanced accuracy. Class imbalance was addressed using standard SMOTE oversampling applied exclusively within training folds, and model interpretability was examined through LIME, SHAP, and Permutation Feature Importance analyses. RESULTS: Stochastic and deterministic optimization strategies showed no statistically or clinically meaningful difference in severity classification performance, with mean CV F1-scores of 0.693 and 0.694 respectively (difference [Formula: see text]) (formally confirmed by equivalence testing with TOST, [Formula: see text], [Formula: see text]). Random Search demonstrated the highest efficiency (17.992 evaluations/minute), followed by Grid Search (16.734), while Genetic Algorithms exhibited the lowest efficiency (5.952), requiring over 41 minutes on average. Algorithm selection and dataset characteristics dominated performance variability, accounting for 5.5% and 90.6% of total variance respectively, while optimization strategy contributed approximately 0% (variance component collapsed to zero under formal mixed-effects decomposition, consistent with negligible influence). After class balancing and hyperparameter tuning, Random Forest achieved the highest performance on the severity classification task (F1-score: 0.831, ROC AUC: 0.870). Explainability analyses consistently identified age, coca-cola urine, prostration, hyperpyrexia, and convulsions as key predictive features across multiple methods. CONCLUSIONS: Simple hyperparameter optimization strategies may be sufficient for model development in malaria severity classification applications under the tested conditions, achieving performance comparable to more complex methods with substantially lower computational requirements. Clinical utility, however, remains to be confirmed through prospective validation. The findings support a pragmatic modeling approach prioritizing data quality, algorithm selection, and computational efficiency over optimization complexity, particularly relevant for resource-constrained healthcare settings where malaria burden is highest. CI - (c) 2026. The Author(s). FAU - Awe, Olushina Olawale AU - Awe OO AD - Statistical Learning Laboratory, Federal University of Bahia, Salvador, Brazil. oawe@unicamp.br. FAU - de Souza, Julia Soares AU - de Souza JS AD - Department of Statistics, State University of Campinas, Campinas, Brazil. FAU - Bittencourt Cruz, Erson Kaue AU - Bittencourt Cruz EK AD - Department of Statistics, State University of Campinas, Campinas, Brazil. FAU - Adekunle, Olukorede Olabanji AU - Adekunle OO AD - Division of Neurosurgery, Department of Surgery, University of Ilorin Teaching Hospital, Ilorin, Kwara State, Nigeria. olukorede.adekunle@npmcn.edu.ng. FAU - Owolabi, Paul J AU - Owolabi PJ AD - Covenant Applied Informatics and Communications Africa Center of Excellence (CApiC-ACE), Covenant University, Ota, Nigeria. FAU - Oladeji, Emmanuel Olusola AU - Oladeji EO AD - Surgery Interest Group of Africa, Lagos, Nigeria. LA - eng PT - Journal Article DEP - 20260801 PL - England TA - BMC Med Inform Decis Mak JT - BMC medical informatics and decision making JID - 101088682 SB - IM MH - Humans MH - *Malaria/diagnosis MH - *Severity of Illness Index MH - Predictive Learning Models MH - *Machine Learning MH - Classification Algorithms MH - Random Forest MH - Boosting Machine Learning Algorithms MH - Prediction Algorithms MH - *Clinical Decision-Making/methods PMC - PMC13715095 OTO - NOTNLM OT - Hyperparameter optimization OT - Machine learning OT - Malaria severity classification OT - Model explainability OT - SMOTE COIS- Declarations. Ethics approval and consent to participate: This study used a de-identified clinical dataset previously collected and published by Awe et al. [39], which received ethics approval from the relevant institutional review board at the time of original data collection. The present secondary analysis used only the published, anonymised data in accordance with the terms of the original study. No new patient data were collected for this work. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/08/01 CRDT- 2026/10/28 00:43 PHST- 2026/03/19 00:00 [received] PHST- 2026/07/23 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:43 [entrez] PHST- 2026/08/01 00:00 [pmc-release] AID - 10.1186/s12911-026-03732-6 [pii] AID - 3732 [pii] AID - 10.1186/s12911-026-03732-6 [doi] PST - epublish SO - BMC Med Inform Decis Mak. 2026 Aug 1;26(1):440. doi: 10.1186/s12911-026-03732-6. PMID- 42947166 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 2050-7771 (Print) IS - 2050-7771 (Electronic) IS - 2050-7771 (Linking) VI - 14 IP - 1 DP - 2026 Oct 27 TI - Contrasting molecular and pharmacodynamic signatures of response and resistance to sequential bortezomib and temozolomide in recurrent IDH-wildtype glioblastoma and grade 4 IDH-mutant astrocytoma: an exploratory interim analysis of NCT03643549 phase IB/II trial. LID - 10.1186/s40364-026-01023-w [doi] LID - 148 AB - BACKGROUND: The revision of the WHO classification of CNS tumours now distinguishes grade 4 diffuse gliomas by their molecular features, but tailored treatment is yet to be standardised. Glioblastoma (isocitrate dehydrogenase (IDH))-wildtype tumours harbouring an unmethylated O(6)-methylguanine-DNA methyltransferase "(uMGMT)" promoter are inherently resistant to temozolomide (TMZ). However, no standardised treatment recommendations are available for grade 4 astrocytoma IDH-mutant. We previously demonstrated that administration of bortezomib (BTZ) 48 h prior to TMZ depletes MGMT protein and sensitizes uMGMT glioma cells to TMZ. Based on these findings, a phase IB/II trial was initiated to evaluate the safety and clinical activity of sequential BTZ and TMZ. This interim analysis aimed to determine a futility safeguard, identify molecular and pharmacodynamic determinants of treatment response and failure in the first 15 patients consecutively treated. METHODS: Adult patients with recurrent grade 4 IDH-mutant astrocytoma or IDH-wildtype glioblastoma, uMGMT promoter, measurable disease, Karnofsky Performance Status >/= 70, and disease progression >/= 12 weeks after radiotherapy were enrolled. Patients received intravenous BTZ 1.3 mg/m(2) on days 1, 4, and 7 of each 28-day cycle, followed by oral TMZ 200 mg/m(2)/day for 5 consecutive days beginning on day 3. Targeted sequencing of glioma-associated genes and longitudinal plasma proteomic profiling using LC-MS/MS were performed before, during, and after treatment. RESULTS: Fifteen patients (median age 52 years) comprised 10 patients with IDH- wildtype glioblastoma and 5 patients with grade 4 IDH-mutant astrocytoma. Disease control (partial response and stable disease) was observed in 4/10 (40%) IDH- wildtype glioblastoma patients, comprising one PR and three cases of SD. In contrast, all five patients with grade 4 IDH-mutant astrocytoma experienced rapid progressive disease. Notably, non-responding IDH-wildtype glioblastomas and all IDH-mutant tumours harboured alterations affecting the CDKN2A/RB1-cell-cycle regulatory axis, including CDKN2A and/or RB1loss, with one IDH-mutant patient additionally harbouring PIK3CA gain. By comparison, responding IDH-wildtype glioblastomas were characterized by amplified EGFR, nuclear NF-kappaB (p65/RelA) activation, and absence of RB-pathway alterations. Longitudinal proteomic analyses demonstrated comparatively attenuated treatment-induced changes in proteins associated with invasion and extracellular matrix remodelling in responders, whereas proteins involved in damage clearance immune phenotype, platelet activation, aggregation, and degranulation were significantly altered during disease control. CONCLUSIONS: Sequential BTZ and TMZ therapy was feasible with manageable hematologic and non-hematologic toxicities and met prespecified interim futility criteria, supporting continued recruitment to the phase II study. Disease control was restricted to a molecularly defined subset of IDH- wildtype glioblastoma characterized by EGFR amplification and NF-kappaB activation. In contrast, universal treatment failure among grade 4 IDH-mutant astrocytomas was associated with alterations of the CDKN2A/RB1 cell-cycle checkpoint pathway, suggesting that advanced molecular evolution and cell-cycle deregulation may override the survival advantage typically associated with IDH-mutant gliomas and limit the effectiveness of MGMT-targeted sensitization strategies. REGISTRY: ClinicalTrials.gov trial registration number: NCT03643549, date of registration: 2018-08-21. CI - (c) 2026. The Author(s). FAU - Goplen, Dorota AU - Goplen D AD - Department of Oncology, Haukeland University Hospital, Bergen, Norway. AD - Department of Clinical Medicine, University of Bergen, Bergen, Norway. FAU - Rahman, Mohummad Aminur AU - Rahman MA AD - Department of Oncology, Haukeland University Hospital, Bergen, Norway. AD - Department of Biomedicine, University of Bergen, Bergen, Norway. FAU - Brekke, Jorunn AU - Brekke J AD - Department of Oncology, Haukeland University Hospital, Bergen, Norway. FAU - Waha, Andreas AU - Waha A AD - Department of Neuropathology, University of Bonn, Bonn, Germany. FAU - Oltedal, Leif AU - Oltedal L AD - Department of Clinical Medicine, University of Bergen, Bergen, Norway. AD - Mohn Medical Imaging and Visualization Centre, Department of Radiology, Haukeland University Hospital, Bergen, Norway. FAU - Haasz, Judit AU - Haasz J AD - Mohn Medical Imaging and Visualization Centre, Department of Radiology, Haukeland University Hospital, Bergen, Norway. FAU - Kumar, Surendra AU - Kumar S AD - Newfoundland and Labrador Health Services, Department of Research and Innovation, Newfoundland, Canada. FAU - Birkeland, Even AU - Birkeland E AD - Department of Biomedicine, University of Bergen, Bergen, Norway. FAU - Selheim, Frode AU - Selheim F AD - Department of Biomedicine, University of Bergen, Bergen, Norway. FAU - Lie, Stein Atle AU - Lie SA AD - Department of Clinical Dentistry, University of Bergen, Bergen, Norway. FAU - Chekenya, Martha AU - Chekenya M AUID- ORCID: 0000-0001-7241-3451 AD - Department of Biomedicine, University of Bergen, Bergen, Norway. martha.chekenya@biomed.uib.no. LA - eng SI - ClinicalTrials.gov/NCT03643549 GR - # 190170/Norwegian Cancer Society/ PT - Journal Article DEP - 20261027 PL - England TA - Biomark Res JT - Biomarker research JID - 101607860 PMC - PMC13715206 OTO - NOTNLM OT - CDKN2A/RB1-cell-cycle regulatory axis OT - MGMT methylation OT - Activated NFkB OT - Amplified EGFR OT - Proteasome inhibitor COIS- Declarations. Ethics approval and consent to participate: This multicentre, open-label phase IB/II trial, was registered on 2018-08-21 as BORTEM-17 on ClinicalTrials.gov (ID NCT03643549). The study was performed in line with the principles of the Declaration of Helsinki. The study protocol was approved by the Regional Committee for Medical and Health Research Ethics for Western Norway, (Regionale komiteer for medisinsk og helsefaglig forskningsetikk (REK), Vest-Norge (REK Vest)) (2017/2084) on 18.12.2017 and The Norwegian Medical Products Agency (DMP) (formerly the Norwegian Medicines Agency) (17/17445-17). Written informed consent was obtained from all individual participants included in the study. Consent for publication: All authors read and consented to publication of the final version of the manuscript. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/27 CRDT- 2026/10/28 00:42 PHST- 2026/07/02 00:00 [received] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:42 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 10.1186/s40364-026-01023-w [pii] AID - 1023 [pii] AID - 10.1186/s40364-026-01023-w [doi] PST - epublish SO - Biomark Res. 2026 Oct 27;14(1):148. doi: 10.1186/s40364-026-01023-w. PMID- 42947160 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1749-8090 (Electronic) IS - 1749-8090 (Linking) VI - 21 IP - 1 DP - 2026 Jul 31 TI - Bibliometric and visualized analysis of research trends and knowledge structure in novel pharmacological treatments for hypertrophic cardiomyopathy. LID - 10.1186/s13019-026-04555-2 [doi] LID - 854 AB - BACKGROUND: This study presents a bibliometric and visual analysis of the literature on novel drug therapies for hypertrophic cardiomyopathy (HCM) to explore recent research trends, hotspots, and developmental directions. METHODS: Publications related to novel drug treatments for HCM from 2015 to 2025 were retrieved from the Web of Science Core Collection. CiteSpace and VOSviewer (with standard parameters: time slicing 2015-2025, node selection based on co-occurrence, and pathfinder pruning) were utilized for quantitative and visual analyses. RESULTS: After screening, 281 publications were included. The annual publication output exhibited an overall upward trend. The United States led in the number of publications, while England played a prominent hub role in international collaboration. The Harvard University system formed a high-yield cluster, and the University of California system served as a key bridge. Co-citation analysis of journals revealed an interdisciplinary knowledge structure spanning basic science to clinical medicine. Both reference and keyword analyses confirmed that the research frontier has shifted from early exploration of the cardiac myosin mechanism to a current focus on myosin inhibitors. CONCLUSION: Bibliometric evidence indicates a clear shift toward targeted therapy centered on cardiac myosin inhibitors, marking a transition in research focus. These findings provide a quantitative roadmap for researchers and clinicians to identify key players, collaborative opportunities, and emerging topics. Future studies are expected to deepen focus on non-obstructive HCM, personalized treatment, and long-term efficacy verification. CI - (c) 2026. The Author(s). FAU - Chen, Zhen AU - Chen Z AD - Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430014, China. FAU - Ai, Fen AU - Ai F AD - Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430014, China. FAU - Li, Jinlei AU - Li J AD - Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430014, China. FAU - Cheng, Bingxin AU - Cheng B AD - Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430014, China. FAU - Cheng, Weihao AU - Cheng W AD - Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430014, China. FAU - Li, Yu AU - Li Y AD - Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430014, China. 1297661619@qq.com. LA - eng PT - Journal Article PT - Review DEP - 20260731 PL - England TA - J Cardiothorac Surg JT - Journal of cardiothoracic surgery JID - 101265113 SB - IM MH - *Cardiomyopathy, Hypertrophic/drug therapy MH - Humans MH - *Bibliometrics MH - *Biomedical Research/trends PMC - PMC13715134 OTO - NOTNLM OT - Bibliometrics OT - Hypertrophic Cardiomyopathy OT - Novel Pharmacological Treatments OT - Research Trends COIS- Declarations. Ethics approval and consent to participate: This study is a bibliometric analysis of publicly available scientific literature and did not involve direct interaction with human subjects or access to private health information. Therefore, ethical approval and informed consent were not required for this study. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:42 PHST- 2025/11/27 00:00 [received] PHST- 2026/07/10 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:42 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s13019-026-04555-2 [pii] AID - 4555 [pii] AID - 10.1186/s13019-026-04555-2 [doi] PST - epublish SO - J Cardiothorac Surg. 2026 Jul 31;21(1):854. doi: 10.1186/s13019-026-04555-2. PMID- 42947158 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1546-0096 (Electronic) IS - 1546-0096 (Linking) VI - 24 IP - 1 DP - 2026 Jul 31 TI - Outcomes of arthrocentesis and arthroscopy in the management of TMJ involvement in juvenile idiopathic arthritis: a retrospective analysis. LID - 10.1186/s12969-026-01254-z [doi] LID - 81 AB - BACKGROUND: Temporomandibular joint (TMJ) involvement in juvenile idiopathic arthritis (JIA) is common, frequently underrecognized, and may progress despite minimal symptoms. Untreated TMJ arthritis has been associated with pain, functional limitation, and dentofacial growth disturbance, supporting early identification and intervention even in clinically subtle disease. The role of minimally invasive TMJ procedures remains incompletely defined. The aim of this study was to evaluate patient-reported and clinical outcomes following TMJ arthrocentesis or arthroscopy in patients with symptomatic TMJ involvement associated with JIA. METHODS: We retrospectively evaluated 45 patients with JIA who underwent TMJ arthrocentesis or arthroscopy between 2014 and 2023. The cohort was 96% female, with a mean age of 15.7 years at first intervention (range, 6.0-23.0 years). TMJ arthrocentesis was performed in 27% of study subjects and arthroscopy in 73%; intra-articular corticosteroid injection was administered in 80%. Outcomes included the patient-completed 20-item Jaw Functional Limitation Scale (JFLS-20), pain measures including the Characteristic Pain Index (CPI) derived from the Graded Chronic Pain Scale, and maximal interincisal opening (MIO) at early (0-3 months), intermediate (3-9 months), and late (9-24 months) follow-up. Regression analyses identified improvement predictors and need for additional surgical intervention. RESULTS: Minimally invasive TMJ procedures were associated with significant early and intermediate improvements in function, pain, and mouth opening (p < 0.05). These benefits diminished over time, and recurrent symptoms were common, with 20 study subjects (44%) requiring additional procedures during follow-up. Procedure type and JIA subtype were not associated with outcomes. MRI-confirmed synovitis, greater baseline functional limitation, DMARD use, and younger age predicted greater early improvement. Higher number of tender sites predicted both early pain improvement and increased likelihood of re-intervention. CONCLUSIONS: Minimally invasive TMJ procedures provide meaningful but often temporary symptomatic relief in JIA-associated TMJ arthritis. High re-intervention rates likely reflect the complex and recurrent nature of TMJ symptoms in this population. These findings support the selective adjunctive use of local TMJ interventions within a multidisciplinary, systemically focused treatment strategy aimed at mitigating symptomatic burden and potential long-term sequelae. CI - (c) 2026. The Author(s). FAU - Akerman, Eyal AU - Akerman E AD - Department of Oral and Maxillofacial Surgery, Sheba Medical Center, Tel- Hashomer, Israel. akermaneyal@gmail.com. FAU - McLean, Ryan AU - McLean R AD - Department of Oral and Maxillofacial Surgery, School of Dentistry, University of Michigan, Ann Arbor, MI, USA. FAU - Troost, Jonathan AU - Troost J AD - Department of Biostatistics, University of Michigan, Ann Arbor, MI, USA. FAU - Anderson, Sara AU - Anderson S AD - Department of Oral and Maxillofacial, Denver Health, Colorado, USA. FAU - Edwards, Sean P AU - Edwards SP AD - Department of Oral and Maxillofacial Surgery, School of Dentistry, University of Michigan, Ann Arbor, MI, USA. FAU - Hakim, Mohamed AU - Hakim M AD - Private Practice, The International Center for TMJ and Jaw Surgery, VA, Arlington, USA. FAU - Ash, Jennifer AU - Ash J AD - Department of Oral and Maxillofacial Surgery, School of Dentistry, University of Michigan, Ann Arbor, MI, USA. FAU - Aronovich, Sharon AU - Aronovich S AD - Department of Oral and Maxillofacial Surgery, School of Dentistry, University of Michigan, Ann Arbor, MI, USA. LA - eng PT - Journal Article DEP - 20260731 PL - England TA - Pediatr Rheumatol Online J JT - Pediatric rheumatology online journal JID - 101248897 SB - IM MH - Humans MH - Female MH - *Arthritis, Juvenile/complications MH - Retrospective Studies MH - *Arthroscopy/methods MH - *Arthrocentesis/methods MH - Adolescent MH - Child MH - Male MH - *Temporomandibular Joint Disorders/surgery/etiology MH - Treatment Outcome MH - Young Adult MH - Pain Measurement MH - Temporomandibular Joint/surgery MH - Injections, Intra-Articular PMC - PMC13712084 OTO - NOTNLM OT - Arthritis OT - Arthrocentesis OT - Arthroscopy OT - Juvenile idiopathic arthritis OT - Temporomandibular joint COIS- Declarations. Ethics approval and consent to participate: This retrospective study was approved by the University of Michigan Institutional Review Board (Study ID: HUM00113676) and was defined as exempt from the requirement to obtain informed consent from individual participants. Consent for publication: Not applicable. This manuscript does not contain any individual person's data in any form. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:42 PHST- 2026/04/10 00:00 [received] PHST- 2026/07/22 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:42 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s12969-026-01254-z [pii] AID - 1254 [pii] AID - 10.1186/s12969-026-01254-z [doi] PST - epublish SO - Pediatr Rheumatol Online J. 2026 Jul 31;24(1):81. doi: 10.1186/s12969-026-01254-z. PMID- 42947157 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1472-6947 (Electronic) IS - 1472-6947 (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Machine learning‑based risk stratification for moderate‑to‑severe anxiety‑depression in patients with hypertension and/or coronary heart disease: a multicenter cross‑sectional study. LID - 10.1186/s12911-026-03734-4 [doi] LID - 439 AB - BACKGROUND: Anxiety and depression in patients with hypertension and/or coronary heart disease (CHD) are associated with severe outcomes. However, there is a lack of objective risk stratification tools. This proof‑of‑concept study developed eight machine learning (ML) models to assess the feasibility of risk stratification for moderate‑to‑severe anxiety‑depression in this population and to identify key associated factors. METHODS: The study retrospectively included patients with hypertension and/or CHD from the 2023 Psychology and Behavior Investigation of Chinese Residents (PBICR) database, a cross‑sectional, self‑report survey. Patients were randomly split into training (70%) and test (30%) sets for internal validation. In the training set, least absolute shrinkage and selection operator (LASSO) regression with cross-validation was employed to select features for constructing the model. Eight ML models were developed for risk stratification of moderate‑to‑severe anxiety‑depression (PHQ‑9 >/= 10 and/or GAD‑7 >/= 10). Model performance was assessed using the area under the receiver operating characteristic curve (AUROC), calibration, and decision curve analysis (DCA). RESULTS: The final cohort comprised 4,006 eligible patients, allocated to training (n = 2,805) and test (n = 1,201) sets. LASSO regression selected 15 predictors from 43 initial features. This study established eight ML models for risk stratification of moderate-to-severe anxiety-depression in patients with hypertension and/or CHD. Random Forest (RF) demonstrated optimal discrimination in the training set (AUROC = 0.837; 95% CI: 0.812-0.862). In the test set, it maintained acceptable discrimination (AUROC = 0.838; 95% CI: 0.814-0.862; Brier score = 0.134). Family health, risk taking, and quality of life (QoL) were identified as the strongest correlates via SHAP analysis. DCA suggested net benefit across risk thresholds of 10-40%. CONCLUSIONS: The RF model achieved the best performance, with family health, risk taking, and QoL as the strongest correlates. The proposed two‑stage screening workflow is hypothetical and has not been prospectively validated. Due to a lack of external validation and the cross‑sectional, self‑report design, this study is hypothesis‑generating and proof‑of‑concept. Prospective validation in independent cohorts is required before any clinical application. CI - (c) 2026. The Author(s). FAU - Wang, Dandan AU - Wang D AD - Department of Nursing, Qilu Hospital, Shandong University, Jinan, China. AD - Interventional Catheter Center, Qilu Hospital of Shandong University, Jinan, China. FAU - Wang, Faying AU - Wang F AD - Department of Nursing, Qilu Hospital, Shandong University, Jinan, China. AD - Interventional Catheter Center, Qilu Hospital of Shandong University, Jinan, China. FAU - Chen, Liang AU - Chen L AD - Department of Emergency, Qilu Hospital of Shandong University, Jinan, China. chenliang@qiluhospital.cn. LA - eng PT - Journal Article PT - Multicenter Study DEP - 20260731 PL - England TA - BMC Med Inform Decis Mak JT - BMC medical informatics and decision making JID - 101088682 SB - IM MH - Humans MH - Female MH - Cross-Sectional Studies MH - Male MH - *Coronary Disease/epidemiology/psychology MH - Middle Aged MH - Risk Assessment/methods MH - *Hypertension/epidemiology/psychology MH - *Machine Learning MH - *Depression/diagnosis/epidemiology MH - *Anxiety/diagnosis/epidemiology MH - Retrospective Studies MH - Aged MH - Predictive Learning Models MH - Adult MH - Classification Algorithms MH - Random Forest PMC - PMC13715117 OTO - NOTNLM OT - Anxiety OT - Coronary heart disease OT - Depression OT - Hypertension OT - Machine learning OT - Risk stratification model COIS- Declarations. Ethics approval and consent to participate: This cross-sectional study was conducted in accordance with the Declaration of Helsinki, approved by the Ethics Review Committee of Shandong Provincial Hospital (SWYX: NO.2023 - 198), and registered in the Chinese Clinical Trial Registry (registration number: ChiCTR2300072573) on 16 June 2023. Written informed consent was obtained from all participants before participation. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:42 PHST- 2025/12/16 00:00 [received] PHST- 2026/07/24 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:42 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s12911-026-03734-4 [pii] AID - 3734 [pii] AID - 10.1186/s12911-026-03734-4 [doi] PST - epublish SO - BMC Med Inform Decis Mak. 2026 Jul 31;26(1):439. doi: 10.1186/s12911-026-03734-4. PMID- 42947154 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1747-597X (Electronic) IS - 1747-597X (Linking) VI - 21 IP - 1 DP - 2026 Jul 29 TI - Initiation, appeal, and dependence: a qualitative exploration of e-cigarette use in Chile. LID - 10.1186/s13011-026-00757-y [doi] LID - 71 AB - BACKGROUND: E-cigarette use has increased in Chile, where products remain widely accessible through diverse sales channels, including informal markets. Lifetime prevalence is 15.1%, with 3.6% reporting past-year use. Use is highest among young adults (19-25 years) and lowest among older groups. Despite Chile's historically high smoking prevalence among women, national data show no sex differences in past-year e-cigarette use. Although nicotine-containing devices are classified as pharmaceutical products, no brand has obtained formal authorization for sale. This study aims to examine how adults engage with e-cigarettes in the Chilean regulatory context, focusing on patterns of initiation, product selection, and sustained use. METHODS: Eighteen semi-structured in-depth interviews were conducted between May 2024 and 2025 with adult e-cigarette users (including one informal seller) in the Metropolitan Region of Santiago, Chile, in a convenience sample. Participants were recruited through snowball sampling and outreach across social and digital platforms. Data were transcribed, anonymized, and thematically analyzed using an inductive approach to capture participants' experiences. RESULTS: Participants were primarily young adults (aged 18-45), with a predominance of women (77.8%). Four interrelated themes emerged: (1) initiation often took place in social and recreational settings, where peer sharing and informal access were common; (2) users frequently prioritized design, flavor, and ease of use over nicotine content, framing e-cigarettes as lifestyle products; (3) occasional use sometimes progressed to routine consumption, facilitated by convenience and environmental factors; and (4) signs of dependence and reported strategies to moderate their use. CONCLUSIONS: E-cigarette use was closely tied to social settings, aesthetic appeal, and daily routines. Product engagement was shaped more by usability and context than by nicotine content or health motivations. These findings highlight the need for policies that address informal sales channels, improve product transparency, and consider the social and sensory drivers of e-cigarette use in underregulated contexts. CI - (c) 2026. The Author(s). FAU - Silva-Gallardo, Constanza AU - Silva-Gallardo C AD - Department of Psychology, Faculty of Psychology and Humanities, Universidad San Sebastian, Santiago, Chile. constanza.silva@uss.cl. AD - Millennium Nucleus for the Evaluation and Analysis of Drug Policies (nDP), Santiago, Chile. constanza.silva@uss.cl. FAU - Astudillo, Gabriel AU - Astudillo G AD - Facultad College, Pontificia Universidad Catolica de Chile, Santiago, Chile. AD - Escuela de Salud Publica, Facultad de Medicina, Pontificia Universidad Catolica de Chile, Santiago, Chile. FAU - Concha, Francisca AU - Concha F AD - Departamento de Antropologia, Facultad de Ciencias Sociales, Universidad de Chile, Santiago, Chile. FAU - Rocha-Jimenez, Teresita AU - Rocha-Jimenez T AD - Society and Health Research Center, Escuela de Gobierno y Administracion Publica, Universidad Mayor, Santiago, Chile. AD - SENTINET: Surveillance, Epidemiology, and New Technologies for Infectious Emerging (CIN250062), Santiago, Chile. LA - eng GR - FONDECYT de Iniciacion No.11240213/Agencia Nacional de Investigacion y Desarrollo/ GR - FONDECYT Regular No. 1231102 and Anillos No. ATE230065/Agencia Nacional de Investigacion y Desarrollo/ GR - NCS2021_003/Millennium Science Initiative Program, Chile/ PT - Journal Article DEP - 20260729 PL - England TA - Subst Abuse Treat Prev Policy JT - Substance abuse treatment, prevention, and policy JID - 101258060 SB - IM MH - Humans MH - Chile/epidemiology MH - Female MH - Adult MH - *Electronic Nicotine Delivery Systems/statistics & numerical data MH - Qualitative Research MH - Young Adult MH - Male MH - Adolescent MH - Middle Aged MH - *Vaping/epidemiology/psychology PMC - PMC13715092 OTO - NOTNLM OT - E-cigarette OT - Health perception OT - Latin America OT - Nnicotine OT - Public policy OT - Qualitative study OT - Regulation OT - Vaping COIS- Declarations. Ethics approval and consent to participate: This study was approved by the Ethics Committee for Research on Humans of Universidad Mayor (Resolution No. 0447). All participants provided written informed consent prior to participation, including consent for audio recording and the use of anonymized quotes in publications. Ethical procedures were conducted in accordance with institutional guidelines and the principles of the Declaration of Helsinki. Consent for publication: This manuscript includes anonymized excerpts from interview transcripts. All participants provided written informed consent for the use and publication of their responses, in accordance with the procedures approved by the institutional ethics committee. Declaration of generative AI and AI-assisted technologies in the writing process: During the preparation of this work, the author(s) used ChatGPT Scholar to improve readability and grammar. After using this tool/service, the author(s) reviewed and edited the content as needed and take(s) full responsibility for the content of the publication. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/29 CRDT- 2026/10/28 00:42 PHST- 2025/12/04 00:00 [received] PHST- 2026/07/26 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:42 [entrez] PHST- 2026/07/29 00:00 [pmc-release] AID - 10.1186/s13011-026-00757-y [pii] AID - 757 [pii] AID - 10.1186/s13011-026-00757-y [doi] PST - epublish SO - Subst Abuse Treat Prev Policy. 2026 Jul 29;21(1):71. doi: 10.1186/s13011-026-00757-y. PMID- 42947153 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1743-0003 (Electronic) IS - 1743-0003 (Linking) VI - 23 IP - 1 DP - 2026 Jul 30 TI - How many repeated observations are enough for digital gait summaries? A multi-dataset framework for sampling sufficiency in older adults. LID - 10.1186/s12984-026-02109-0 [doi] LID - 310 AB - BACKGROUND: Repeated digital gait and mobility assessments are increasingly summarized into indicators at the participant level, yet the number of repeated observations needed for different summary families to support stable interpretation remains unclear. We developed and evaluated a multi-dataset framework to estimate sampling sufficiency for repeated digital gait and mobility summaries in older adults. METHODS: Four publicly available datasets were analyzed. Three datasets with stride observations spanning optical motion capture, foot-mounted inertial sensing, and cross-modality inertial/video recordings served as the development tier based on stride observations, and one dataset based on Timed Up and Go (TUG) served as a supportive transfer dataset with repeated phase- and trial-level observations. Five summary families at the participant level were compared: Mean, Top10Mean, Bottom10Mean, Best2, and Worst2. For each combination of dataset, feature, and summary family, repeated subsampling within each participant was used to estimate the minimum stable observation count relative to each participant's reference based on all available data, using a prespecified median absolute percentage error criterion (MdAPE). Downstream analyses of associations with anchors were restricted to summaries that met the stability criterion. We then performed a focused comparison between Mean and Bottom10Mean in the primary dataset with stride observations and representative inferential-stability analyses under repeated subsampling. RESULTS: Across datasets with stride observations, minimum stable observation counts depended strongly on summary family and sensing context. Mean generally required the fewest observations, whereas numerical-tail and extreme summaries defined by feature orientation required substantially denser repeated sampling and did not consistently reach threshold across all features. After stability qualification, selected noncentral summaries retained statistically supported associations with functional, frailty-related, and fall-related anchors. In Dataset 2, the lower-tail summary Bottom10Mean retained information beyond the corresponding mean for a subset of feature-anchor combinations. In the dataset based on TUG, all evaluated summary families reached the stability criterion with lower observation burden than in the datasets with stride observations, providing supportive evidence that the same analytical logic was also supported in a different setting with repeated observations. Repeated subsampling further showed that insufficient observation support primarily attenuated downstream effect estimates and increased uncertainty rather than causing frequent directional reversals. CONCLUSIONS: Repeated digital gait and mobility summaries should not be assumed to be interchangeable. Their analytical and potential translational interpretation should depend on whether sufficient repeated observations have been collected for the intended summary family. Beyond gait analysis, the framework may help guide protocol design, data quality control, and summary selection in repeatedly sampled clinical data. CI - (c) 2026. The Author(s). FAU - Jiang, Linjing AU - Jiang L AD - School of Nursing, Shandong Xiehe University, Jinan, 250109, Shandong, China. jlinjing@outlook.com. AD - Institutes of Innovation for Future Society, Nagoya University, Nagoya, Aichi, 4648601, Japan. jlinjing@outlook.com. FAU - Shu, Haolu AU - Shu H AD - Faculty of Sport Sciences, Waseda University, Saitama, Tokorozawa, 359-1192, Japan. FAU - Wiloth, Stefanie AU - Wiloth S AD - Institute for Ageing Research (IAF), OST, Eastern Switzerland University of Applied Sciences, Rosenbergstrasse 59, 9001, St. Gallen, Switzerland. FAU - Husler, Samira-Salome AU - Husler SS AD - Institute for Ageing Research (IAF), OST, Eastern Switzerland University of Applied Sciences, Rosenbergstrasse 59, 9001, St. Gallen, Switzerland. FAU - Zhang, Pei AU - Zhang P AD - School of Nursing, Shandong Xiehe University, Jinan, 250109, Shandong, China. FAU - Shen, Chunhong AU - Shen C AD - School of Nursing, Shandong Xiehe University, Jinan, 250109, Shandong, China. FAU - Wang, Ruowei AU - Wang R AD - School of Nursing, Shandong Xiehe University, Jinan, 250109, Shandong, China. wangruowei@sdxiehe.edu.cn. FAU - Kasahara, Satoshi AU - Kasahara S AD - Faculty of Health Sciences, Hokkaido University, Sapporo, Hokkaido, 060-0812, Japan. LA - eng PT - Journal Article DEP - 20260730 PL - England TA - J Neuroeng Rehabil JT - Journal of neuroengineering and rehabilitation JID - 101232233 SB - IM MH - Humans MH - Aged MH - *Gait/physiology MH - Male MH - Female MH - Motion Capture MH - *Gait Analysis/methods MH - Aged, 80 and over PMC - PMC13715267 OTO - NOTNLM OT - Digital gait assessment OT - Digital mobility outcomes OT - Older adults OT - Rehabilitation OT - Repeated observations OT - Sampling sufficiency OT - Stability-qualified associations OT - Wearable sensors COIS- Declarations. Ethics approval and consent to participate: Not applicable. This study used publicly available, de-identified datasets and did not involve new recruitment or direct interaction with human participants. Consent for publication: Not applicable. Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/30 CRDT- 2026/10/28 00:42 PHST- 2026/04/17 00:00 [received] PHST- 2026/07/22 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:42 [entrez] PHST- 2026/07/30 00:00 [pmc-release] AID - 10.1186/s12984-026-02109-0 [pii] AID - 2109 [pii] AID - 10.1186/s12984-026-02109-0 [doi] PST - epublish SO - J Neuroeng Rehabil. 2026 Jul 30;23(1):310. doi: 10.1186/s12984-026-02109-0. PMID- 42947145 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1479-5876 (Electronic) IS - 1479-5876 (Linking) VI - 24 IP - 1 DP - 2026 Jul 30 TI - The clinical value of (68)Ga-FAPI to evaluate the peritoneal fibrosis and its inherent molecule relationship with POSTN and DPP7. LID - 10.1186/s12967-026-08721-x [doi] LID - 1358 AB - BACKGROUND: Peritoneal fibrosis(PF) is a major cause of peritoneal membrane failure in peritoneal dialysis(PD) patients, lacking clear occurrence mechanism and reliable non-invasive diagnostic tools. Our study investigated the basic and clinical evidence of fibroblast activation protein (FAP) to detect and quantify PD-associated PF and related potential molecular protein. METHODS: On the one hand, we evaluated the capacity of FAP in the process of peritoneal fibrosis, including in vitro experiment of human peritoneal mesothelial cells (HPMCs) dataset analysis of GSE162029, and a murine PF model established by daily intraperitoneal injection of 4.25% glucose solution for 2-4 weeks. Finally, (68)Ga-FAPI-04 PET/CT imaging was performed in animal models and patients. On the other hand, we detected the expression of periostin(POSTN), dipeptidyl peptidase 7(DPP7), and glucagon-like peptide-1 receptor(GLP-1 R) in model animals. RESULTS: Increased FAP expression in HPMCs was in a dose-dependent manner (p < 0.01). Transcriptomic analysis confirmed FAP upregulation in glucose-exposed murine peritoneum. In the PF mouse model, with peritoneal transport function deteriorating over time (p < 0.05), histology revealed elevated FAP expression strongly related to indicators of fibrosis. Crucially, (68)Ga-FAPI-04 PET/CT demonstrated significantly increased peritoneal maximum standardized uptake value(SUV(max)) in PF mice compared to controls(p < 0.05). Surprisingly, in patients undergoing dialysis for more than 24 months, a positive (68)Ga-FAPI-04 PET scan result was inversely correlated with Kt/V(p < 0.05). POSTN and DPP7 were elevated in model animals (p < 0.05). CONCLUSION: FAP expression is significantly elevated during PD-associated PF development and the results of (68)Ga-FAPI-04 PET/CT, reflected peritoneal functional status and the degree of fibrosis to some extent. Meanwhile, the increase of POSTN and DPP7 were in positive correlation with FAP. The findings represents a promising transformative diagnostic tool for early detection and monitoring of PF and potential mechanism under PF. TRIAL REGISTRATION: ChiCTR: ChiCTR2300079318. Registered 31 December 2023, https://www.chictr.org.cn/showproj.html?proj=214233. CI - (c) 2026. The Author(s). FAU - Xiao, Kaiying AU - Xiao K AD - Department of Nephrology, Xijing Hospital, The Fourth Military Medical University, No. 127 Changle West Road, Xi'an, 710032, China. AD - Grade 2021 Clinical Medicine (Five-Year Program), Basic Medical College, The Fourth Military Medical University, Xi'an, China. FAU - Zhang, Yuting AU - Zhang Y AD - Department of Nephrology, Xijing Hospital, The Fourth Military Medical University, No. 127 Changle West Road, Xi'an, 710032, China. FAU - Peng, Hongpin AU - Peng H AD - Department of Nephrology, Xijing Hospital, The Fourth Military Medical University, No. 127 Changle West Road, Xi'an, 710032, China. FAU - Niu, Meng AU - Niu M AD - Department of Nuclear Medicine, Xijing Hospital, The Fourth Military Medical University, Xi'an, China. FAU - Li, Ganze AU - Li G AD - Department of Medical Microbiology and Parasitology, The Fourth Military Medical University, Xi'an, China. FAU - Xu, Xiaomeng AU - Xu X AD - Department of Nephrology, Xijing Hospital, The Fourth Military Medical University, No. 127 Changle West Road, Xi'an, 710032, China. FAU - Zhang, Xizhi AU - Zhang X AD - Department of Nephrology, Xijing Hospital, The Fourth Military Medical University, No. 127 Changle West Road, Xi'an, 710032, China. FAU - Yang, Qianye AU - Yang Q AD - Grade 2021 Clinical Medicine (Five-Year Program), Basic Medical College, The Fourth Military Medical University, Xi'an, China. FAU - Zhao, Ya AU - Zhao Y AD - Department of Medical Microbiology and Parasitology, The Fourth Military Medical University, Xi'an, China. FAU - He, Lijie AU - He L AD - Department of Nephrology, Xijing Hospital, The Fourth Military Medical University, No. 127 Changle West Road, Xi'an, 710032, China. helijie@fmmu.edu.cn. LA - eng PT - Journal Article DEP - 20260730 PL - England TA - J Transl Med JT - Journal of translational medicine JID - 101190741 RN - EC 3.4.21.- (Fibroblast Activation Protein Alpha) RN - 0 (Periostin) RN - EC 3.4.- (Endopeptidases) RN - EC 3.4.24.- (Gelatinases) RN - 0 (68Ga-FAPI) RN - EC 3.4.14.- (Dipeptidyl-Peptidases and Tripeptidyl-Peptidases) RN - 0 (Membrane Proteins) RN - EC 3.4.21.- (Serine Endopeptidases) RN - 0 (Gallium Radioisotopes) RN - 0 (POSTN protein, human) RN - 0 (Quinolines) RN - 0 (Cell Adhesion Molecules) SB - IM MH - Animals MH - Humans MH - Fibroblast Activation Protein Alpha MH - *Peritoneal Fibrosis/diagnostic imaging/metabolism/pathology/genetics MH - *Periostin/metabolism MH - Endopeptidases MH - Positron Emission Tomography Computed Tomography MH - *Gelatinases/metabolism MH - *Dipeptidyl-Peptidases and Tripeptidyl-Peptidases/metabolism MH - *Membrane Proteins/metabolism MH - Male MH - *Serine Endopeptidases/metabolism MH - Peritoneum/pathology MH - Disease Models, Animal MH - *Gallium Radioisotopes MH - Female MH - Mice MH - Mice, Inbred C57BL MH - Quinolines MH - Cell Adhesion Molecules PMC - PMC13715138 OTO - NOTNLM OT - 68Ga-FAPI OT - DPP7 OT - Fibroblast Activation Protein (FAP) OT - POSTN OT - Peritoneal Fibrosis COIS- Declarations. Ethics approval and consent to participate: This clinical trial was conducted at Xijing Hospital and the ethical approval number is KY20232347-F-1 admitted by the Ethics Committee of the First Affiliated Hospital of the Air Force Medical University of the People's Liberation Army of China. All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. And all the participants signed the informed consent form. This animal experiment was reviewed and approved by the Animal Ethics Committee of Air Force Military University. The study design complied with the "3 R" principles (Replacement, Reduction, Refinement) for laboratory animal use, and strictly adhered to: the Guidelines for the Use and Management of Laboratory Animalsissued by the National Institutes of Health (NIH) and the Implementation Rules for the Management of Medical Laboratory Animals promulgated by the National Health and Family Planning Commission of China. Consent for publication: Written informed consent was obtained from all individual participants included in the study. The consent form explicitly stated that their data (including any identifiable images or clinical details) could be used for publication in scientific journals. Registration of the clinical trial-ChiCTR: Name of the registry: Clinical study on early diagnosis and progression monitoring of peritoneal fibrosis using FAPI-PET imaging. Trial registration number:ChiCTR2300079318, Date of registration: Dec 30, 2023, URL of trial registry, record: https://www.chictr.org.cn/showproj.html?proj=214233 Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/30 CRDT- 2026/10/28 00:41 PHST- 2026/03/17 00:00 [received] PHST- 2026/07/17 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:41 [entrez] PHST- 2026/07/30 00:00 [pmc-release] AID - 10.1186/s12967-026-08721-x [pii] AID - 8721 [pii] AID - 10.1186/s12967-026-08721-x [doi] PST - epublish SO - J Transl Med. 2026 Jul 30;24(1):1358. doi: 10.1186/s12967-026-08721-x. PMID- 42947143 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1742-4690 (Electronic) IS - 1742-4690 (Linking) VI - 23 IP - 1 DP - 2026 Jul 23 TI - Virus-informed targeting of HIV reservoirs: Rev-dependent HIV Immunotherapy (RHIT). LID - 10.1186/s12977-026-00682-9 [doi] LID - 12 AB - Persistence of viral reservoirs prevents a cure or functional cure for HIV. Efforts to eradicate viral reservoirs have been constrained by the difficulties of identifying and eliminating rare, heterogeneous viral reservoir cells in vivo. Single-modality interventions such as "shock and kill" have faced persistent challenges despite decades of effort, highlighting the need for complementary strategies that more precisely target infected cells. In this review, I summarize our 20 years of effort in developing an HIV Rev-dependent lentiviral vector for selective targeting of viral reservoirs. This lentiviral vector expresses therapeutic genes under the control of HIV Tat and Rev, conferring high specificity. In vitro, superinfection of HIV-infected cells with the Rev-dependent particle can lead to the identification and selective killing of infected cells. In vivo, injection of Rev-dependent lentiviral particles into SIV-infected rhesus macaques stimulates antiviral immunity, leading to reduction of viral reservoirs and inhibition of viral rebound. We designate this Rev vector-based therapeutic approach as Rev-dependent HIV Immunotherapy (RHIT). The RHIT approach integrates three complementary reservoir-targeting strategies: (1) RHIT-mediated killing of reservoir cells via Rev-dependent therapeutic gene expression; (2) RHIT-mediated inhibition of active HIV replication via defective interfering particles; and (3) RHIT-induced anti-HIV immunity via induction of neutralizing antibodies and cell-mediated immune responses. In this review, I also discuss limitations and challenges for the future development of RHIT. CI - (c) 2026. The Author(s). FAU - Wu, Yuntao AU - Wu Y AD - Center for Infectious Disease Research, George Mason University, Manassas, VA, 20110, USA. ywu8@gmu.edu. LA - eng GR - R01AI148012/National Institute of Allergy and Infectious Diseases/ PT - Journal Article PT - Review DEP - 20260723 PL - England TA - Retrovirology JT - Retrovirology JID - 101216893 RN - 0 (rev Gene Products, Human Immunodeficiency Virus) SB - IM MH - Humans MH - *HIV Infections/therapy/virology/immunology MH - Animals MH - *Immunotherapy/methods MH - *rev Gene Products, Human Immunodeficiency Virus/genetics MH - Genetic Vectors MH - *HIV-1/genetics/physiology/immunology MH - Lentivirus/genetics MH - Host-Directed Therapy MH - Virus Latency PMC - PMC13712061 OTO - NOTNLM OT - HIV cure OT - HIV functional cure OT - HIV-1 OT - Immunotherapy OT - Reservoir OT - Rev OT - Rev-dependent vector OT - Shock and kill OT - Tat COIS- Declarations. Competing interests: Yuntao Wu is an Editorial Board Member of Retrovirology. The author declares no other competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/23 CRDT- 2026/10/28 00:41 PHST- 2026/04/30 00:00 [received] PHST- 2026/07/10 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:41 [entrez] PHST- 2026/07/23 00:00 [pmc-release] AID - 10.1186/s12977-026-00682-9 [pii] AID - 682 [pii] AID - 10.1186/s12977-026-00682-9 [doi] PST - epublish SO - Retrovirology. 2026 Jul 23;23(1):12. doi: 10.1186/s12977-026-00682-9. PMID- 42947141 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1472-6955 (Print) IS - 1472-6955 (Electronic) IS - 1472-6955 (Linking) VI - 25 IP - 1 DP - 2026 Jul 30 TI - Recognizing the victim, missing the procedure: a phenomenologically informed qualitative study of forensic midwifery awareness. LID - 10.1186/s12912-026-05109-6 [doi] LID - 1026 AB - INTRODUCTION: This study aimed to explore midwifery students' baseline perceptions and perceived preparedness regarding forensic midwifery prior to formal elective education, and how they interpret suspected forensic cases within their developing professional identity. METHOD: A phenomenologically informed qualitative design was used. Sixteen third-year midwifery students in Turkiye were selected through purposive sampling. Data were collected through remote (one-to-one) semi-structured interviews conducted via video call between 01 and 10 August 2025. The interviews were audio-recorded, transcribed verbatim, and analyzed using meaning-oriented thematic analysis. Codes were grouped into subthemes and themes reflecting meaning patterns in students' narratives. Rigor was supported through independent coding by two researchers, consensus on themes, use of direct quotations, and adherence to SRQR guidelines. The forensic midwifery elective had not been offered to this cohort; therefore, none of the participants had taken/completed it at the time of the interviews. RESULTS: Four main themes emerged: (1) the holistic role of midwives from victim protection to justice, (2) dilemmas related to knowledge, competence, and legal responsibility, (3) the empowering role of education and the unique position of midwives in close contact with women and children, and (4) educational and societal strategies to increase awareness. Students recognized their responsibility to identify forensic cases, protect victims, and report incidents. However, they reported uncertainty regarding reporting/referral pathways and basic evidence-protection principles (minimizing contamination), highlighting the need for foundational undergraduate training. Participants emphasized that midwives' close contact with women, infants, and children provides a critical advantage in recognizing victimization. They strongly recommended including forensic midwifery education in the undergraduate curriculum and highlighted academic activities, in-service training, and social media as tools to improve awareness. CONCLUSION: Students' awareness of forensic midwifery was shaped more by ethical responsibility and professional role perception than by procedural knowledge. Although students could identify suspected forensic cases, they described limited perceived preparedness regarding practical and legal procedures required for effective initial response. CI - (c) 2026. The Author(s). FAU - Acavut, Gamze AU - Acavut G AUID- ORCID: 0000-0001-5493-128X AD - Department of Nursing, Faculty of Health Sciences, Cankiri Karatekin University, Cankiri, Turkiye. gmzkyl86@yahoo.com. FAU - Arikan, Birsen AU - Arikan B AUID- ORCID: 0000-0002-2153-1156 AD - Department of Midwifery, Faculty of Health Sciences, Ankara Medipol University, Ankara, Turkiye. FAU - Cifci, Suheda AU - Cifci S AUID- ORCID: 0009-0004-7287-8151 AD - Department of Midwifery, Faculty of Health Sciences, Ankara Medipol University, Ankara, Turkiye. LA - eng PT - Journal Article DEP - 20260730 PL - England TA - BMC Nurs JT - BMC nursing JID - 101088683 PMC - PMC13711806 OTO - NOTNLM OT - Baseline awareness OT - Forensic midwifery OT - Midwifery students OT - Phenomenologically informed qualitative study OT - Qualitative research COIS- Declarations. Ethics approval and consent to participate: Ethical approval for this study was obtained from the Ankara Medipol University Non-Interventional Clinical Research Ethics Committee (Ankara Medipol University) (Decision No: 139; Date: 30 July 2025). The study was conducted in accordance with the principles of the Declaration of Helsinki. Written informed consent to participate (including consent for audio-recording) was obtained from all participants prior to the interviews. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/30 CRDT- 2026/10/28 00:41 PHST- 2026/02/25 00:00 [received] PHST- 2026/07/21 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:41 [entrez] PHST- 2026/07/30 00:00 [pmc-release] AID - 10.1186/s12912-026-05109-6 [pii] AID - 5109 [pii] AID - 10.1186/s12912-026-05109-6 [doi] PST - epublish SO - BMC Nurs. 2026 Jul 30;25(1):1026. doi: 10.1186/s12912-026-05109-6. PMID- 42947140 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1479-5876 (Electronic) IS - 1479-5876 (Linking) VI - 24 IP - 1 DP - 2026 Oct 17 TI - Mitochondrial dysfunction in diabetic neuropathy: a core pathogenic hub and therapeutic implications. LID - 10.1186/s12967-026-09015-y [doi] LID - 1354 AB - BACKGROUND: Diabetic neuropathy is a heterogeneous complication of diabetes in which sensory loss, neuropathic pain, autonomic dysfunction and distal axonal degeneration cannot be fully explained by hyperglycaemia alone. Because peripheral axons, Schwann cells and autonomic neurons depend on sustained bioenergetic support, mitochondrial injury has emerged as a central link between metabolic overload, neuroinflammation and progressive nerve dysfunction. MAIN BODY: This review synthesizes evidence that hyperglycaemia, lipotoxicity and microvascular stress impair electron transport, oxidative phosphorylation and mitochondrial DNA stability, leading to excessive reactive oxygen species generation, calcium dyshomeostasis, mitochondrial permeability transition pore opening and maladaptive metabolic reprogramming. Disrupted fission-fusion balance, defective axonal transport, impaired mitophagy and abnormal mitochondria-associated endoplasmic reticulum membranes further weaken mitochondrial quality control in long peripheral axons. These changes engage SARM1-related NAD+ depletion, inflammasome activation, cGAS-STING signalling, apoptosis and glial-neuronal metabolic failure, thereby connecting bioenergetic collapse with pain sensitisation, distal axonal degeneration and autonomic dysfunction. Across painful and painless diabetic peripheral neuropathy, corneal small-fibre neuropathy, autonomic neuropathy and diabetes-related central nervous system injury, mitochondrial mechanisms appear shared, but endpoints and dominant cell types differ. Translationally, exercise and dietary strategies, metabolic regulators, SARM1 and calcium-pathway targeting, mitophagy modulation, mitochondria-directed antioxidants, and traditional Chinese medicine or natural products are discussed as metabolism-oriented interventions. Current evidence supports target engagement in experimental systems, but human validation, tissue exposure data, structural nerve endpoints and high-quality disease-modifying trials remain insufficient. CONCLUSION: Mitochondrial dysfunction functions as a core pathogenic hub-rather than the sole cause-linking substrate overload, redox imbalance, calcium stress, organelle quality-control failure, inflammatory signalling, and axonal degeneration in diabetic neuropathy. The most actionable translational path is not symptomatic antioxidant use alone but biomarker-guided, mechanism-stratified trials that connect mitochondrial target modulation with small-fibre structure, nerve function, safety, and patient-centred outcomes. SEARCH STRATEGY AND EVIDENCE SELECTION: For this narrative review, PubMed was searched from database inception to 1 August 2026, supplemented by backward reference screening of relevant original studies and recent reviews. Search terms included "diabetic neuropathy", "diabetic peripheral neuropathy", "mitochondria", "oxidative phosphorylation", "oxidative stress", "mitophagy", "SARM1", "NLRP3", "cGAS-STING", and "mitochondria-associated endoplasmic reticulum membranes", alone and in combination. English-language original mechanistic, translational, observational, and interventional studies were prioritised; recent high-quality reviews were used for context and to identify primary studies. Evidence from other neurological disorders was retained only when direct DN evidence was limited and the study clarified biological plausibility. Because this is a narrative review, no formal risk-of-bias tool or GRADE assessment was applied; human, animal, and cellular evidence and major translational limitations are instead distinguished explicitly. CI - (c) 2026. The Author(s). FAU - Wu, Xiaotong AU - Wu X AD - Department of Endocrinology and Metabolism, The Second Qilu Hospital of Shandong University, Jinan, 250033, China. FAU - Li, Xiaobo AU - Li X AD - Department of Endocrinology and Metabolism, The Second Qilu Hospital of Shandong University, Jinan, 250033, China. FAU - Lou, Nengjun AU - Lou N AD - Department of Endocrinology and Metabolism, The Second Qilu Hospital of Shandong University, Jinan, 250033, China. FAU - Zhuang, Xianghua AU - Zhuang X AD - Department of Endocrinology and Metabolism, The Second Qilu Hospital of Shandong University, Jinan, 250033, China. zhuangxianghua@email.sdu.edu.cn. FAU - Chen, Shihong AU - Chen S AUID- ORCID: 0000-0002-5488-2139 AD - Department of Endocrinology and Metabolism, The Second Qilu Hospital of Shandong University, Jinan, 250033, China. chenshihong@sdu.edu.cn. LA - eng PT - Journal Article PT - Review DEP - 20261017 PL - England TA - J Transl Med JT - Journal of translational medicine JID - 101190741 SB - IM MH - Humans MH - *Mitochondria/pathology/metabolism MH - *Diabetic Neuropathies/therapy/pathology/metabolism MH - Animals PMC - PMC13711899 OTO - NOTNLM OT - Diabetic neuropathy OT - Mitochondrial dysfunction OT - Mitophagy OT - Neuroinflammation OT - Oxidative stress COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: This manuscript has been read and approved by all the authors to publish and is not submitted or under consideration for publication elsewhere. Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/17 CRDT- 2026/10/28 00:41 PHST- 2026/06/17 00:00 [received] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:41 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - 10.1186/s12967-026-09015-y [pii] AID - 9015 [pii] AID - 10.1186/s12967-026-09015-y [doi] PST - epublish SO - J Transl Med. 2026 Oct 17;24(1):1354. doi: 10.1186/s12967-026-09015-y. PMID- 42947122 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1742-4755 (Electronic) IS - 1742-4755 (Linking) VI - 23 IP - 1 DP - 2026 Jul 31 TI - Effect of mobile health application interventions on modern contraceptive use among women of reproductive age in sub-Saharan Africa: a systematic review. LID - 196 [pii] LID - 10.1186/s12978-026-02415-9 [doi] AB - INTRODUCTION: Sustainable Development Goal 3, target seven, aims to ensure universal access to sexual and reproductive health care services, including family planning information and education. Mobile health interventions may be an effective means to achieve this target. Mobile health (mhealth) involves the use of mobile phone applications (MPAs) and text messages (SMS) on devices such as cell phones to provide relevant information to improve well-being and health. Given the growing interest in the use of mobile health to improve healthcare delivery, especially in sub-Saharan Africa (SSA), up-to-date information must be available to guide practice. OBJECTIVES: To provide up-to-date evidence on the effectiveness of mhealth interventions in enhancing contraceptive use among women of reproductive age in SSA. METHODS: We conducted a systematic review of the literature in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-analysis guidelines. The review was guided by a protocol registered in PROSPERO and aimed to provide up-to-date evidence on the effectiveness of mhealth interventions in enhancing contraceptive use. The PICO framework guided the literature search, and the review was conducted with an information specialist. The literature search covered studies published from 1994 to 2022 in the following databases: Medline, CINAHL, PubMed CENTRAL, the Cochrane Library, and EMBASE. Two independent reviewers conducted the screening in three stages. The title, abstract, and full-text were screened to identify studies that met the inclusion criteria. Data were extracted using a standardised data extraction form, synthesised, and assessed for quality. The quality of randomised controlled trials (RCTs) was evaluated using the JBI critical appraisal tool for RCTs. The JBI critical appraisal tool for cross-sectional studies was used to assess the non-randomised studies. A meta-analysis was conducted for studies that met the meta-analysis criteria. RESULTS: A total of 9,768 records were retrieved following the searches. Eight (8) studies were included in the final review: 6 RCTs, and 2 cross-sectional studies. A positive effect of mhealth on modern contraceptive use was reported in 4 out of the 8 studies, while the remaining 4 did not report any significant effect. A meta-analysis of data pooled from the 6 RCTs demonstrated a statistically significant effect of the intervention on contraceptive use (OR = 1.46, 95% CI: 1.11-1.92), indicating a meaningful increase in the likelihood of use among participants exposed to mhealth interventions. CONCLUSIONS: The effect of mhealth applications on modern contraceptive use was inconclusive based on vote counting among the 8 selected studies. However, findings from the meta-analysis of 6 RCT studies appear to suggest that mobile health interventions can significantly improve contraceptive use among women of reproductive age. CI - (c) 2026. The Author(s). FAU - Adjei, Kwame Kesse AU - Adjei KK AD - Department of Population, Family and Reproductive Health, School of Public Health, University of Ghana, Accra, Ghana. kwamekadjei@gmail.com. AD - Centre for Global Health and Bioethics Research, Bono East, Ghana. kwamekadjei@gmail.com. FAU - Ganle, John AU - Ganle J AD - Department of Population, Family and Reproductive Health, School of Public Health, University of Ghana, Accra, Ghana. FAU - Boadu, Isaac AU - Boadu I AD - Department of Population, Family and Reproductive Health, School of Public Health, University of Ghana, Accra, Ghana. FAU - Intiful, Freda AU - Intiful F AD - Department of Dietetics, University of Ghana, Legon, Ghana. FAU - Kotoh, Agnes M AU - Kotoh AM AD - Department of Population, Family and Reproductive Health, School of Public Health, University of Ghana, Accra, Ghana. FAU - Yawson, Alfred E AU - Yawson AE AD - Department of Community Health, University of Ghana Medical School, Legon, Ghana. FAU - Mwaisaka, Jeffrey AU - Mwaisaka J AD - Department of Population, Family and Reproductive Health, School of Public Health, University of Ghana, Accra, Ghana. FAU - Adjei, Irene Apewe AU - Adjei IA AD - Kintampo Health Research Centre, Kintampo, Ghana. FAU - Akparibo, Robert AU - Akparibo R AD - Sheffield Centre for Health and Related Research, School of Medicine and Population Health, University of Sheffield, Sheffield, England. LA - eng PT - Journal Article PT - Review PT - Systematic Review DEP - 20260731 PL - England TA - Reprod Health JT - Reproductive health JID - 101224380 SB - IM MH - Humans MH - Female MH - Africa South of the Sahara MH - *Telemedicine MH - *Contraception Behavior/statistics & numerical data MH - Sub-Saharan African People MH - *Mobile Applications MH - *Family Planning Services MH - *Contraception MH - Cell Phone MH - Text Messaging OTO - NOTNLM OT - Contraceptives OT - Effect OT - Mobile health OT - Sub-Saharan Africa OT - Women COIS- Declarations. Ethics approval and consent to participate: None. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 00:40 PHST- 2025/08/28 00:00 [received] PHST- 2026/07/19 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:40 [entrez] AID - 10.1186/s12978-026-02415-9 [pii] AID - 10.1186/s12978-026-02415-9 [doi] PST - epublish SO - Reprod Health. 2026 Jul 31;23(1):196. doi: 10.1186/s12978-026-02415-9. PMID- 42947118 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1475-2867 (Print) IS - 1475-2867 (Electronic) IS - 1475-2867 (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Uncovering LINC02982: a T-ALL-specific lncrna linking epigenetic regulation and metabolic pathways. LID - 10.1186/s12935-026-04433-w [doi] LID - 352 AB - T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive pediatric malignancy with limited molecular biomarkers to guide diagnosis and personalized therapy. Given the critical role of long non-coding RNAs in gene regulation and chromatin dynamics, we investigated whether novel lncRNAs could contribute to T-ALL pathogenesis. Through transcriptomic profiling of patient samples and in-silico validation on public datasets, we identified LINC02982 as a lncRNA selectively overexpressed in T-ALL and specifically enriched in leukemic blasts. Functional silencing of LINC02982 in multiple T-ALL cell lines impaired proliferation, altered cell-cycle progression, and increased apoptosis. Integrative ATAC-seq and RNA-seq analyses revealed widespread changes in chromatin accessibility and gene expression, including deregulation of metabolic genes such as AK4 and PSAT1. Promoter accessibility showed a moderate correlation with transcriptional changes, consistent with a potential cis-regulatory role, while broader transcriptomic alterations suggest additional trans effects. Pathway analysis highlighted metabolic processes, including cholesterol biosynthesis, as significantly affected, with LINC02982 depletion reducing expression of key pathway genes and intracellular cholesterol levels. These findings identify LINC02982 as a T-ALL-associated lncRNA linked to transcriptional and metabolic alterations, supporting its potential biological relevance in leukemic cellular regulation and warranting further investigation in larger clinical cohorts. CI - (c) 2026. The Author(s). FAU - Pecoraro, Giovanni AU - Pecoraro G AD - IRCCS SYNLAB SDN, Via G. Ferraris 144, Naples, 80146, Italy. FAU - Pane, Katia AU - Pane K AD - IRCCS SYNLAB SDN, Via G. Ferraris 144, Naples, 80146, Italy. FAU - Franzese, Monica AU - Franzese M AD - IRCCS SYNLAB SDN, Via G. Ferraris 144, Naples, 80146, Italy. FAU - Beneduce, Giuliana AU - Beneduce G AD - Department of Pediatric Hemato-Oncology, Santobono-Pausilipon Children's Hospital, AORN, Naples, 80122, Italy. FAU - De Matteo, Antonia AU - De Matteo A AD - Department of Pediatric Hemato-Oncology, Santobono-Pausilipon Children's Hospital, AORN, Naples, 80122, Italy. FAU - Parasole, Rosanna AU - Parasole R AD - Department of Pediatric Hemato-Oncology, Santobono-Pausilipon Children's Hospital, AORN, Naples, 80122, Italy. FAU - Smaldone, Giovanni AU - Smaldone G AD - IRCCS SYNLAB SDN, Via G. Ferraris 144, Naples, 80146, Italy. giovanni.smaldone@synlab.it. LA - eng GR - Ricerca Corrente/Ministero della Salute/ GR - Ricerca Corrente/Ministero della Salute/ GR - Ricerca Corrente/Ministero della Salute/ GR - GR-2021-12372945/Ministero della Salute/ PT - Journal Article DEP - 20260731 PL - England TA - Cancer Cell Int JT - Cancer cell international JID - 101139795 PMC - PMC13711947 OTO - NOTNLM OT - Acute lymphoblastic leukemia of T-cells OT - Biomarker OT - Epigenetic OT - LncRNA OT - Multiomics COIS- Declarations. Ethics approval and consent to participate: The procedures followed in this study, in line with the Helsinki declaration, have been approved by the local ethical committees of the IRCCS-SDN (Ethical Committee IRCCS Pascale, Naples, Italy-protocol number 5/19 of the 19/06/2019) and 1/23 (dated 8 February 2023), as well as by the AORN Santobono-Pausilipon Ethics Committee (Cardarelli/Pausilipon, Naples, Italy) under protocol number 07/20 (dated 3 June 2020). Patient consent for publication: Both parents signed informed consent for the participation to the study and for data publication. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:40 PHST- 2025/12/15 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:40 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s12935-026-04433-w [pii] AID - 4433 [pii] AID - 10.1186/s12935-026-04433-w [doi] PST - epublish SO - Cancer Cell Int. 2026 Jul 31;26(1):352. doi: 10.1186/s12935-026-04433-w. PMID- 42947111 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1471-2482 (Electronic) IS - 1471-2482 (Linking) VI - 26 IP - 1 DP - 2026 Aug 1 TI - Clinical experience of transumbilical single-port laparoscopic cholecystectomy using a modified glove-port device and conventional instruments. LID - 10.1186/s12893-026-04102-9 [doi] LID - 725 AB - BACKGROUND: The use of single-port laparoscopic cholecystectomy (SPLC) with disposable multichannel ports is constrained in resource-limited primary hospitals under China's diagnosis-related groups (DRGs) payment model, where consumable expenditures are systematically controlled. This study reports a practical modification of the glove-port technique using conventional instruments for transumbilical single-port laparoscopic cholecystectomy (TUSPLC) and compares its single-center clinical outcomes with those of conventional three-port LC. METHODS: A retrospective, non-randomized, comparative cohort analysis was performed on consecutive eligible patients who underwent TUSPLC (study group) or traditional three-port LC (control group) between October 2023 and December 2024 at a single center. Baseline demographics, perioperative outcomes, hospitalization costs, and short-term patient-reported cosmetic satisfaction were compared. Propensity score matching (PSM) was performed to adjust for selection bias on age, sex, BMI, ASA classification, and disease type. RESULTS: A total of 173 patients underwent TUSPLC and 242 patients underwent three-port LC. After nearest-neighbor 1:1 PSM on age, sex, BMI, ASA grade, and gallbladder disease type, 162 pairs were retained for analysis. Matching yielded well-balanced groups (all standardized mean differences below 0.1). Three TUSPLC cases required conversion to multi-port surgery (1.7%) - two because of difficult Calot's triangle dissection and one for severe gallbladder atrophy. No bile duct injury, postoperative hemorrhage, or surgical site infection was recorded in either group. Operative time (42.0 +/- 5.1 vs. 41.2 +/- 4.6 min, mean difference 0.80 min, 95% CI -0.3 to 1.9, p = 0.140), blood loss (5.9 +/- 1.5 vs. 5.7 +/- 1.4 mL, mean difference 0.20 mL, 95% CI -0.1 to 0.5, p = 0.220), and total cost (14,376.2 +/- 982.5 vs. 14,437.3 +/- 856.7 CNY, mean difference - 61.1 CNY, 95% CI - 262.6 to 140.4, p = 0.551) did not differ significantly between the two approaches (all p > 0.05). Postoperative hospital stay was statistically shorter in the TUSPLC group (2.0 +/- 0.5 vs. 2.1 +/- 0.4 days, mean difference - 0.10 day, 95% CI -0.2 to 0.0, p = 0.048), although the absolute difference was small and of uncertain clinical significance. Visual analogue scale (VAS) scores at 1 month favored TUSPLC (9.23 +/- 0.45 vs. 8.52 +/- 0.68, mean difference 0.71, 95% CI 0.6 to 0.8, p < 0.001), although the assessment was unblinded and patient-reported. CONCLUSIONS: TUSPLC using a modified glove-port device and conventional instruments appears technically feasible within the short-term (1-month) follow-up period in selected patients, with no statistically significant differences in operative time, blood loss, or total hospitalization costs compared with conventional three-port LC. Postoperative hospital stay was statistically shorter by 0.10 day, a difference of uncertain clinical significance. This modified technique may serve as a practical option for performing single-port LC in resource-limited primary-level hospitals under China's DRGs payment model. However, because of the retrospective, single-center design and short (1-month) follow-up, long-term safety data for port-site hernia and other delayed complications are lacking, and the cosmetic benefit requires validation with objective assessments; therefore, these findings should be interpreted as preliminary. Further prospective, multicenter studies with extended follow-up are essential. CI - (c) 2026. The Author(s). FAU - Xiong, Shuming AU - Xiong S AD - Department of Hepatobiliary Surgery, The Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, China. FAU - Yang, Jun AU - Yang J AD - Department of Hepatobiliary Surgery, The Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, China. FAU - Zhu, Maoqun AU - Zhu M AD - Department of Hepatobiliary Surgery, The Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, China. FAU - Hu, Benshun AU - Hu B AD - Department of Hepatobiliary Surgery, The Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, China. hubenshun2008@sina.com. LA - eng GR - No. Y20212015/Taihu Zhiguang Science and Technology Project/ GR - No. Q201919/Youth Fund Project of Wuxi Municipal Health Commission/ GR - No. K20253005/Wuxi Science and Technology Development Fund Project/ PT - Comparative Study PT - Journal Article DEP - 20260801 PL - England TA - BMC Surg JT - BMC surgery JID - 100968567 SB - IM MH - Humans MH - *Cholecystectomy, Laparoscopic/instrumentation/methods MH - Female MH - Retrospective Studies MH - Male MH - Umbilicus MH - Middle Aged MH - Adult MH - Operative Time MH - *Gloves, Surgical MH - *Gallbladder Diseases/surgery MH - Equipment Design PMC - PMC13715209 OTO - NOTNLM OT - Cholecystectomy OT - Conventional instruments OT - Custom port OT - Propensity score matching OT - Single-port laparoscopy OT - Transumbilical COIS- Declarations. Ethics approval and consent to participate: This study was approved by the Medical Ethics Committee of The Affiliated Hospital of Jiangnan University (Ethics Approval No. LS2025057). This study was conducted in strict compliance with the ethical principles of the Declaration of Helsinki (2013 revision). Informed written consent to participate in the study and undergo the selected surgical procedure was obtained from all patients and their legal guardians prior to surgery, after a detailed explanation of the study protocol, surgical approaches, potential risks and benefits. Clinical trial number: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/08/01 CRDT- 2026/10/28 00:40 PHST- 2026/01/24 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:40 [entrez] PHST- 2026/08/01 00:00 [pmc-release] AID - 10.1186/s12893-026-04102-9 [pii] AID - 4102 [pii] AID - 10.1186/s12893-026-04102-9 [doi] PST - epublish SO - BMC Surg. 2026 Aug 1;26(1):725. doi: 10.1186/s12893-026-04102-9. PMID- 42947109 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1472-6831 (Electronic) IS - 1472-6831 (Linking) VI - 26 IP - 1 DP - 2026 Aug 1 TI - Marginal gap formation and fracture strength of indirect inlay restorations cemented with preheated resin composites: an in vitro study. LID - 10.1186/s12903-026-09466-1 [doi] LID - 2118 AB - BACKGROUND: Optimal marginal adaptation and adequate fracture strength are critical for the long-term clinical success of indirect posterior restorations. Preheated resin composites have been proposed as alternative luting agents; however, their effects on marginal integrity and mechanical performance of inlays remain unclear. This in vitro study aimed to evaluate gap formation and fracture strength of indirect inlay restorations cemented with preheated resin composites compared with a conventional resin cement. METHODS: Forty human mandibular molar teeth were divided into four groups (n = 10/group). Group 1 used a conventional dual-cure resin cement. Group 2 used a preheated resin composite. Group 3 used a preheated bulk-fill resin composite. Group 4 served as the intact tooth control group and received no cavity preparation or restoration. Standardized Class II MOD inlay preparations were performed, with the mesial proximal box floor located in enamel and the distal box floor located in dentin. Indirect restorations were fabricated from resin nano-ceramic blocks via computer-aided design and computer-aided manufacturing (CAD-CAM). All teeth underwent thermal cycling and chewing simulation. Marginal gap formation was analyzed using micro-computed tomography (micro-CT). Fracture strength was assessed using a universal testing machine, and fracture modes were classified. Data were analyzed using the Shapiro-Wilk test and Levene's test, followed by one-way ANOVA and repeated measures ANOVA with Bonferroni correction and Tukey's post hoc test, and the Fisher-Freeman-Halton test. RESULTS: Enamel margin gap volumes were significantly higher in the preheated bulk-fill composite group compared with the conventional resin cement group (p = 0.012), while no significant differences were observed at dentin margins (p = 0.182). All restored groups exhibited significantly lower fracture strength values compared to the intact teeth group (p = 0.003). Fracture mode distribution showed a tendency toward more repairable fractures in the preheated bulk-fill composite group and more irreparable fractures in the conventional resin cement group. CONCLUSIONS: Preheated resin composites showed comparable performance to conventional resin cement in terms of marginal adaptation and fracture strength. However, their performance appears to be material-dependent. Therefore, preheated resin composites may be considered as potential alternative luting materials for indirect restorations within the limitations of this study. CLINICAL RELEVANCE: Preheated resin-based composites are increasingly considered as alternative luting agents for indirect restorations. Understanding their influence on marginal adaptation and mechanical performance may assist clinicians in selecting appropriate cementation strategies for indirect inlay restorations. CI - (c) 2026. The Author(s). FAU - Isik, Engin AU - Isik E AUID- ORCID: 0009-0008-8597-4969 AD - Department of Restorative Dentistry, School of Dental Medicine, Bahcesehir University, Istanbul, Turkey. engin.isik@bau.edu.tr. FAU - Dalkilic, Evrim Eliguzeloglu AU - Dalkilic EE AUID- ORCID: 0000-0002-1075-9278 AD - Department of Restorative Dentistry, Faculty of Dentistry, Bezmialem Vakif University, Istanbul, Turkey. LA - eng GR - Project No: 20221011/Bezmialem Vakif Universitesi/ GR - Project No: 223S611/Turkiye Bilimsel ve Teknolojik Arastirma Kurumu/ PT - Journal Article DEP - 20260801 PL - England TA - BMC Oral Health JT - BMC oral health JID - 101088684 RN - 0 (Composite Resins) RN - 0 (Resin Cements) SB - IM MH - Humans MH - *Inlays/methods MH - *Composite Resins/chemistry/therapeutic use MH - In Vitro Techniques MH - *Dental Marginal Adaptation MH - Molar MH - *Resin Cements MH - Dental Stress Analysis MH - X-Ray Microtomography MH - Materials Testing MH - Computer-Aided Design MH - *Cementation/methods PMC - PMC13715186 OTO - NOTNLM OT - CAD/CAM OT - Fracture strength OT - Gap formation OT - Indirect restoration OT - Inlay OT - Preheated resin composite COIS- Declarations. Ethics approval and consent to participate: All procedures involving human teeth were performed in accordance with relevant institutional guidelines and regulations and with the ethical principles of the Declaration of Helsinki. Ethical approval for this in vitro study was obtained from the Non-Interventional Research Ethics Committee of Bezmialem Vakif University (approval number: 54022451-050.05.04-79414; date: September 20, 2022). The requirement for informed consent was waived by the Non-Interventional Research Ethics Committee of Bezmialem Vakif University. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/08/01 CRDT- 2026/10/28 00:40 PHST- 2026/05/07 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:40 [entrez] PHST- 2026/08/01 00:00 [pmc-release] AID - 10.1186/s12903-026-09466-1 [pii] AID - 9466 [pii] AID - 10.1186/s12903-026-09466-1 [doi] PST - epublish SO - BMC Oral Health. 2026 Aug 1;26(1):2118. doi: 10.1186/s12903-026-09466-1. PMID- 42947093 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1472-6831 (Electronic) IS - 1472-6831 (Linking) VI - 26 IP - 1 DP - 2026 Aug 1 TI - Comparative evaluation of periodontal biocompatibility of pediatric crowns using clinical parameters and GCF biomarkers. LID - 10.1186/s12903-026-09482-1 [doi] LID - 2119 AB - OBJECTIVES: This study aimed to evaluate clinical and biochemical periodontal responses associated with different crown materials used in primary molars. MATERIALS AND METHODS: A total of 21 children with full crown indication in primary molars (n = 84) were treated with prefabricated zirconia crowns (PZCs), stainless steel crowns (SSCs), and fiberglass crowns (FGCs) in a split-mouth study design; unrestored teeth were considered as the control group. Matrix metalloproteinase-8 (MMP-8) and interleukin-1beta (IL-1beta) levels, plaque index (PI), gingival index (GI), bleeding on probing (BOP) and probing pocket depth (PPD) were evaluated at baseline, 3 months, and 6 months. Continuous outcomes were analyzed using robust mixed analysis of variance, whereas ordinal clinical outcomes were evaluated using generalized estimating equations. RESULTS: PPD did not significantly differ among the groups. GCF volume, MMP-8, and IL-1beta levels showed significant group and time effects. FGCs exhibited significantly higher IL-1beta levels compared to the control, SSC, and PZC groups. MMP-8 levels were significantly higher in the FGC group than in the PZC group, and the group x time interaction indicated increasing MMP-8 levels over time in both the FGC and SSC groups. PZCs demonstrated the most favorable clinical periodontal outcomes, with significantly lower PI, GI, and BOP scores than FGCs during follow-up. CONCLUSIONS: Crown materials were found to affect both clinical periodontal parameters and inflammatory biomarker levels. Among the evaluated materials, PZCs demonstrated the most favorable periodontal profile, whereas FGCs were found to be associated with less favorable clinical and biochemical periodontal outcomes. CLINICAL RELEVANCE: Crown material selection may affect periodontal health and should be carefully considered when planning restorative treatment in pediatric patients. TRIAL REGISTRATION: NCT07611292, 20.05.2026, retrospectively registered. CI - (c) 2026. The Author(s). FAU - Aydinoglu, Sema AU - Aydinoglu S AUID- ORCID: 0000-0003-1490-8645 AD - Faculty of Dentistry, Department of Pediatric Dentistry, Recep Tayyip Erdogan University, Rize, TURKIYE, Turkey. FAU - Sakin Ulubay, Sena AU - Sakin Ulubay S AUID- ORCID: 0000-0003-4086-1502 AD - Faculty of Dentistry, Department of Pediatric Dentistry, Recep Tayyip Erdogan University, Rize, TURKIYE, Turkey. senasakn@gmail.com. FAU - Yemenoglu, Hatice AU - Yemenoglu H AD - Faculty of Dentistry, Department of Periodontology, Recep Tayyip Erdogan University, Rize, Turkiye, Turkey. FAU - Atak, Mehtap AU - Atak M AUID- ORCID: 0000-0002-9451-906X AD - Faculty of Dentistry, Faculty of Medicine, Department of Biochemistry, Recep Tayyip Erdogan University, Rize, TURKIYE, Turkey. FAU - Kose, Oguz AU - Kose O AUID- ORCID: 0000-0002-0318-2458 AD - Faculty of Dentistry, Department of Periodontology, Recep Tayyip Erdogan University, Rize, Turkiye, Turkey. LA - eng SI - ClinicalTrials.gov/NCT07611292 GR - 02026006010392/Recep Tayyip Erdogan University Development Foundation/ GR - TSA-2024-1659/Scientific Research Fund of Recep Tayyip Erdogan University, Rize, Turkey/ PT - Comparative Study PT - Journal Article DEP - 20260801 PL - England TA - BMC Oral Health JT - BMC oral health JID - 101088684 RN - 0 (Biomarkers) RN - EC 3.4.24.34 (Matrix Metalloproteinase 8) RN - 0 (Interleukin-1beta) RN - C6V6S92N3C (Zirconium) RN - S38N85C5G0 (zirconium oxide) RN - 12597-68-1 (Stainless Steel) RN - 0 (fiberglass) RN - 0 (Biocompatible Materials) RN - 0 (Polymers) RN - 7631-86-9 (Silicon Dioxide) SB - IM MH - Humans MH - *Crowns MH - *Gingival Crevicular Fluid/chemistry MH - Female MH - Biomarkers/analysis MH - Matrix Metalloproteinase 8/analysis MH - Interleukin-1beta/analysis MH - Zirconium MH - Male MH - Child MH - Periodontal Index MH - Molar MH - Stainless Steel MH - Tooth, Deciduous MH - Glass MH - *Biocompatible Materials MH - Polymers MH - Silicon Dioxide PMC - PMC13715287 OTO - NOTNLM OT - Fiber OT - GCF OT - IL-1ss OT - MMP-8 OT - SSC OT - Zirconia COIS- Declarations. Ethics approval and consent to participate: The ethics committee approval for the study was obtained from Recep Tayyip Erdogan University Faculty of Medicine Non-Interventional Clinical Research Ethics Committee (Decision no: 2024/05). The procedures used in this study fully comply with the Declaration of Helsinki. Informed consent forms were obtained from parents and patients before the study was conducted. Consent for publication: The authors affirm that human research participants provided informed consent for publication of the images in Fig. 1a-c and Fig. 3. Competing interests: The authors declare no competing interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/08/01 CRDT- 2026/10/28 00:38 PHST- 2026/06/16 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:38 [entrez] PHST- 2026/08/01 00:00 [pmc-release] AID - 10.1186/s12903-026-09482-1 [pii] AID - 9482 [pii] AID - 10.1186/s12903-026-09482-1 [doi] PST - epublish SO - BMC Oral Health. 2026 Aug 1;26(1):2119. doi: 10.1186/s12903-026-09482-1. PMID- 42947092 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1472-6955 (Print) IS - 1472-6955 (Electronic) IS - 1472-6955 (Linking) VI - 25 IP - 1 DP - 2026 Sep 30 TI - Indirect associations involving four work-motivation dimensions between perceived organizational climate and work engagement among geriatric nursing assistants in China: a cross-sectional study. LID - 10.1186/s12912-026-05426-w [doi] LID - 1028 AB - BACKGROUND: Population ageing has increased demand for long-term care, yet evidence on organizational and motivational correlates of work engagement among geriatric nursing assistants in China remains limited. METHODS: Using convenience sampling, this cross-sectional survey recruited geriatric nursing assistants from nine aged care institutions in Xi'an and Weinan, Shaanxi Province, China, between May and August 2025. Of 396 returned questionnaires, 330 were included after prespecified data screening. Participants completed measures of perceived organizational climate, four work-motivation dimensions, and work engagement. Pearson correlations, observed-score regressions, four separate structural equation models, and 5,000-resample bias-corrected bootstrap analyses were conducted. Institution- and covariate-adjusted sensitivity analyses were also performed. RESULTS: In the joint regression model, intrinsic motivation (beta = 0.420, p < 0.001) and introjected regulation (beta = 0.324, p < 0.001) were independently associated with work engagement, whereas perceived organizational climate, identified regulation, and external regulation were not. Across the four structural equation models, perceived organizational climate was positively associated with each motivation dimension (standardized beta = 0.652-0.704), and each motivation dimension was positively associated with work engagement (beta = 0.543-0.771). Because each model included only one motivation dimension, the indirect estimates were model-specific rather than unique contributions independent of the other dimensions. Significant indirect associations were observed through intrinsic motivation (beta = 0.519, bias-corrected 95% CI [0.430, 0.636]), identified regulation (beta = 0.430, [0.349, 0.532]), introjected regulation (beta = 0.530, [0.439, 0.648]), and external regulation (beta = 0.359, [0.258, 0.497]). In separate sensitivity analyses based on standardized observed scores, the four indirect associations remained statistically significant after adjustment for institutions and selected individual-level covariates. CONCLUSIONS: Perceived organizational climate and the four work-motivation dimensions were associated with work engagement among geriatric nursing assistants. The findings support further investigation of how organizational conditions and qualitatively different forms of work motivation relate to engagement. Because all variables were measured concurrently, temporal ordering and causal mediation cannot be established. CI - (c) 2026. The Author(s). FAU - Zhou, Jing AU - Zhou J AD - The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. AD - Yan'an Medical College, Yan'an University, Yan'an, Shaanxi, China. FAU - Zhou, Xi AU - Zhou X AD - The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. zhouxi29@163.com. FAU - Wang, Mengru AU - Wang M AD - The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. AD - Yan'an Medical College, Yan'an University, Yan'an, Shaanxi, China. FAU - Zhang, Jiaojiao AU - Zhang J AD - Yulin Hospital, The First Affiliated Hospital of Xi'an Jiaotong University, Yulin, Shaanxi, China. FAU - Hao, Yan AU - Hao Y AD - The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. AD - Yan'an Medical College, Yan'an University, Yan'an, Shaanxi, China. FAU - Yang, Huiyun AU - Yang H AD - The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. LA - eng GR - 23FZ10/Xi'an Social Science Planning Fund/ PT - Journal Article DEP - 20260930 PL - England TA - BMC Nurs JT - BMC nursing JID - 101088683 PMC - PMC13715160 OTO - NOTNLM OT - Geriatric nursing assistants OT - Indirect association OT - Perceived organizational climate OT - Work engagement OT - Work motivation COIS- Declarations. Ethics approval and consent to participate: This study was conducted in accordance with the Declaration of Helsinki and was approved by the Ethics Committee of the Second Affiliated Hospital of Xi'an Jiaotong University (No. 2025-Lunshen-143). All participants provided informed consent before participation. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/09/30 CRDT- 2026/10/28 00:38 PHST- 2026/05/31 00:00 [received] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:38 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - 10.1186/s12912-026-05426-w [pii] AID - 5426 [pii] AID - 10.1186/s12912-026-05426-w [doi] PST - epublish SO - BMC Nurs. 2026 Sep 30;25(1):1028. doi: 10.1186/s12912-026-05426-w. PMID- 42947090 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1471-2458 (Electronic) IS - 1471-2458 (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Health behavior profiles and potential support needs among community residents with clustered cardiovascular risk factors: a latent profile and explainable machine learning analysis for community-based prevention. LID - 10.1186/s12889-026-28868-y [doi] LID - 3094 AB - BACKGROUND: Community residents with clustered cardiovascular risk factors may exhibit marked heterogeneity in health behavior patterns, posing challenges for community-based cardiovascular prevention and health behavior support. This study aimed to identify subgroups with different levels of health behavior and examine factors associated with subgroup membership using explainable machine learning. METHODS: This secondary analysis used cross-sectional questionnaire data from 475 community residents with clustered cardiovascular risk factors. Health behaviors were assessed using the six dimensions of the Health-Promoting Lifestyle Profile II (HPLP-II). Latent profile analysis (LPA) was used to identify latent health behavior profiles that may reflect different behavioral support needs. The sample was split into training and internal validation sets at an 8:2 ratio. Variable selection was performed using Least Absolute Shrinkage and Selection Operator regression and Random Forest. Five classification models were developed and compared, and the model retained for exploratory analysis was subsequently interpreted using Shapley Additive Explanations (SHAP). RESULTS: Four health behavior subgroups were identified: low health-promoting behavior profile (9.7%), low-to-moderate health-promoting behavior profile (45.5%), moderate-to-high health-promoting behavior profile (34.3%), and high health-promoting behavior profile (10.5%). In the internal validation set, the Random Forest (RF) model showed a comparatively balanced performance across multiple evaluation metrics, with an accuracy of 0.777, Kappa of 0.640, macro-averaged precision of 0.852, macro-averaged F1 score of 0.714, and area under the curve of 0.892; however, its minority-class identification requires further validation. SHAP analysis indicated that health behavior capability, social support, self-efficacy, and age were key contributors to subgroup classification. CONCLUSIONS: Health behaviors among community residents with clustered cardiovascular risk factors exhibit marked heterogeneity. These findings provide descriptive evidence of heterogeneity in health behaviors and may support stratified approaches to health behavior support and community-based cardiovascular prevention strategies. CI - (c) 2026. The Author(s). FAU - Li, Kaihe AU - Li K AD - School of Medicine, Lishui University, No. 1 Xueyuan Road, Lishui, Zhejiang, 323000, China. FAU - Yan, Xinyi AU - Yan X AD - Department of Nursing, The First Affiliated Hospital of Lishui University, Lishui People's Hospital, Lishui, 323000, Zhejiang, China. FAU - Zhou, Ying AU - Zhou Y AD - School of Medicine, Lishui University, No. 1 Xueyuan Road, Lishui, Zhejiang, 323000, China. FAU - Lan, Xuefen AU - Lan X AD - School of Medicine, Lishui University, No. 1 Xueyuan Road, Lishui, Zhejiang, 323000, China. FAU - Li, Xiaofen AU - Li X AD - Department of Nursing, The First Affiliated Hospital of Lishui University, Lishui People's Hospital, Lishui, 323000, Zhejiang, China. FAU - Mai, Juanjuan AU - Mai J AD - School of Medicine, Aksu Vocational and Technical College, Aksu Prefecture, Xinjiang, 843100, China. FAU - Xia, Minqi AU - Xia M AD - School of Medicine, Lishui University, No. 1 Xueyuan Road, Lishui, Zhejiang, 323000, China. FAU - Chen, Ruiming AU - Chen R AD - School of Medicine, Lishui University, No. 1 Xueyuan Road, Lishui, Zhejiang, 323000, China. crming@lsu.edu.cn. LA - eng GR - 2024GYX59/Lishui Municipal Science and Technology Project/ PT - Journal Article DEP - 20260731 PL - England TA - BMC Public Health JT - BMC public health JID - 100968562 SB - IM MH - Humans MH - Female MH - *Health Behavior MH - *Machine Learning MH - Cross-Sectional Studies MH - *Heart Disease Risk Factors MH - Male MH - *Cardiovascular Diseases/prevention & control MH - Middle Aged MH - Surveys and Questionnaires MH - Adult MH - Aged MH - Clustering Algorithms MH - Cluster Analysis MH - Social Support PMC - PMC13715248 OTO - NOTNLM OT - Cardiovascular risk factors OT - Community-based prevention OT - Explainable machine learning OT - Health behavior OT - Latent profile analysis OT - Public health COIS- Declarations. Ethics approval and consent to participate: The present secondary analysis and publication of existing anonymized questionnaire data were reviewed and approved by the Lishui University Ethics Committee (Approval No. 2025R054). The data were derived from a low-risk cross-sectional questionnaire survey conducted between May and August 2024 and were anonymized before analysis. The Ethics Committee confirmed that the use of these existing data for the present analysis and publication was ethically acceptable. The original survey was conducted in accordance with the Declaration of Helsinki. Before data collection, participants were informed of the study purpose, the voluntary nature of participation, and the anonymous use of their responses, and informed consent was obtained from all participants. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:38 PHST- 2026/06/27 00:00 [received] PHST- 2026/07/30 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:38 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s12889-026-28868-y [pii] AID - 28868 [pii] AID - 10.1186/s12889-026-28868-y [doi] PST - epublish SO - BMC Public Health. 2026 Jul 31;26(1):3094. doi: 10.1186/s12889-026-28868-y. PMID- 42947081 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1471-2393 (Electronic) IS - 1471-2393 (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Comparative effectiveness of initiating insulin versus metformin therapy for gestational diabetes mellitus in China: a retrospective cohort study. LID - 10.1186/s12884-026-09752-4 [doi] LID - 1228 AB - BACKGROUND: While insulin is widely recommended as the first-line pharmacological therapy for gestational diabetes mellitus (GDM), the use of metformin is increasing in routine clinical practice. However, real-world evidence comparing their effectiveness and safety remains limited. This study aimed to evaluate glycemic control and adverse pregnancy outcomes between the insulin and the metformin initiation group in women with GDM in China. METHODS: We conducted a retrospective cohort study using electronic medical records from a provincial healthcare database in Eastern China. Women diagnosed with GDM between 2018 and 2025 were included. Patients were divided into the insulin initiation group and the metformin initiation group based on their first prescription. Propensity score overlap weighting (OW) was used to balance baseline characteristics. Weighted linear and logistic regression models were used to assess glycemic outcomes, including the glycated hemoglobin (HbA1c) level at the end of pregnancy, the change in HbA1c, and target achievement (HbA1c < 6.0%). Adverse pregnancy outcomes were evaluated using weighted Cox proportional hazards models with gestational age as the time scale. RESULTS: Of the 7,397 women included, 6,922 (93.6%) initiated insulin and 475 (6.4%) initiated metformin therapy. After OW adjustment, no significant differences were found between the two groups in end-of-pregnancy HbA1c levels, HbA1c changes, or glycemic control rates. Compared with insulin initiation, metformin initiation was associated with significantly lower risks of cesarean delivery (HR: 0.77, 95% CI: 0.62-0.95; P = 0.017), premature rupture of membranes (HR: 0.66, 95% CI: 0.48-0.93; P = 0.016), and macrosomia (HR: 0.61, 95% CI: 0.40-0.93; P = 0.021). Risks for other adverse outcomes, including preterm birth and preeclampsia, were comparable. Sensitivity analyses, including per-protocol analysis, confirmed the robustness of these findings. CONCLUSIONS: In this real-world Chinese cohort, the metformin initiation group achieved glycemic control similar to the insulin initiation group. In addition, the metformin initiation group had lower risks of cesarean delivery, premature rupture of membranes, and macrosomia. These findings suggest that metformin may be a viable and effective initial treatment option for selected women with GDM. CI - (c) 2026. The Author(s). FAU - Jiang, Linfeng AU - Jiang L AD - Department of Pharmaceutical Regulatory Science and Pharmacoeconomics, School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu, 211166, China. FAU - Peng, Nan AU - Peng N AD - School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China. FAU - Xue, Mengyao AU - Xue M AD - Department of Pharmaceutical Regulatory Science and Pharmacoeconomics, School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu, 211166, China. FAU - Wei, Ruolan AU - Wei R AD - Department of Pharmaceutical Regulatory Science and Pharmacoeconomics, School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu, 211166, China. FAU - Lu, Guoxian AU - Lu G AD - Department of Pharmaceutical Regulatory Science and Pharmacoeconomics, School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu, 211166, China. FAU - Fan, Yuqing AU - Fan Y AD - Department of Pharmaceutical Regulatory Science and Pharmacoeconomics, School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu, 211166, China. FAU - Zhang, Shuo AU - Zhang S AD - Department of Pharmaceutical Regulatory Science and Pharmacoeconomics, School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu, 211166, China. FAU - Lyu, Beini AU - Lyu B AD - Institute for Global Health and Development, Peking University, Beijing, 100871, China. Blyu@pku.edu.cn. FAU - Yao, Dongning AU - Yao D AUID- ORCID: 0000-0002-0527-8322 AD - Department of Pharmaceutical Regulatory Science and Pharmacoeconomics, School of Pharmacy, Nanjing Medical University, Nanjing, Jiangsu, 211166, China. dnyao@njmu.edu.cn. AD - Department of Pharmacy, The Second People's Hospital of Changzhou, The Third Affiliated Hospital of Nanjing Medical University, Changzhou, Jiangsu, 213004, China. dnyao@njmu.edu.cn. LA - eng PT - Comparative Study PT - Journal Article DEP - 20260731 PL - England TA - BMC Pregnancy Childbirth JT - BMC pregnancy and childbirth JID - 100967799 RN - 9100L32L2N (Metformin) RN - 0 (Insulin) RN - 0 (Hypoglycemic Agents) RN - 0 (Glycated Hemoglobin) RN - 0 (Blood Glucose) SB - IM MH - Humans MH - Female MH - Pregnancy MH - *Diabetes, Gestational/drug therapy/blood MH - Retrospective Studies MH - *Metformin/therapeutic use MH - China/epidemiology MH - *Insulin/therapeutic use MH - *Hypoglycemic Agents/therapeutic use MH - Adult MH - Glycated Hemoglobin/analysis MH - Pregnancy Outcome MH - Blood Glucose PMC - PMC13711749 OTO - NOTNLM OT - Gestational diabetes mellitus OT - Glycemic control OT - Insulin OT - Pregnancy outcomes OT - Retrospective cohort study OT - metformin COIS- Declarations. Ethics approval and consent to participate: The study protocol was reviewed and approved by the Institutional Review Board of Nanjing Medical University (Approval Number: 20250141). Given that the study utilized anonymized electronic hospital records and did not involve any direct patient contact, the requirement for informed consent was not required. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:38 PHST- 2026/03/08 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:38 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s12884-026-09752-4 [pii] AID - 9752 [pii] AID - 10.1186/s12884-026-09752-4 [doi] PST - epublish SO - BMC Pregnancy Childbirth. 2026 Jul 31;26(1):1228. doi: 10.1186/s12884-026-09752-4. PMID- 42947080 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1471-2482 (Electronic) IS - 1471-2482 (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Preperitoneal desufflation after peritoneal closure during TAPP repair: an intraoperative feasibility study. LID - 10.1186/s12893-026-04104-7 [doi] LID - 724 AB - BACKGROUND: After peritoneal closure in transabdominal preperitoneal (TAPP) inguinal hernia repair, direct visual control of mesh positioning is lost, although dynamic changes within the preperitoneal space continue. This limitation may be clinically relevant in contemporary non-fixation strategies using three-dimensional meshes. This study evaluated the feasibility and intraoperative utility of controlled preperitoneal desufflation (CPD) performed after peritoneal closure during TAPP repair. METHODS: This prospective feasibility study included consecutive adult patients undergoing elective non-fixation TAPP inguinal hernia repair between February 2024 and June 2025. After peritoneal closure, CPD was performed by gradual evacuation of preperitoneal gas under direct laparoscopic visualization. Feasibility, success rate, need for intraoperative corrections, and immediate safety parameters were assessed descriptively. RESULTS: A total of 157 repairs were analyzed. CPD was attempted in 141 repairs (89.8%) and successfully completed in 132 cases (93.6%). Intraoperative corrections were required in 50 repairs (35.5%), most commonly due to inferior mesh edge folding and hernia sac invagination. Most corrections were resolved with a single correction cycle. Peritoneal suture disruption occurred in four repairs (2.8%) and did not require reopening of the peritoneal flap. Final mesh position was considered acceptable in all repairs in which CPD was attempted. Surgeon-reported usefulness was high. CONCLUSIONS: Controlled preperitoneal desufflation is a feasible and reproducible adjunct to TAPP repair that restores intraoperative control over final mesh positioning and enables correction of unfavorable configurations before completion of surgery. CI - (c) 2026. The Author(s). FAU - Mitura, Kryspin AU - Mitura K AUID- ORCID: 0000-0002-2227-4550 AD - Faculty of Medical and Health Sciences, University of Siedlce, Siedlce, Poland. chirurgia.siedlce@gmail.com. AD - Department of General Surgery, Hospital in Siedlce, Siedlce, Poland. chirurgia.siedlce@gmail.com. FAU - Kisielewski, Krystian AU - Kisielewski K AD - Department of General Surgery, Hospital in Siedlce, Siedlce, Poland. FAU - Kacprzak, Laura AU - Kacprzak L AD - Faculty of Medical and Health Sciences, University of Siedlce, Siedlce, Poland. AD - Department of General Surgery, Hospital in Siedlce, Siedlce, Poland. FAU - Wojcik, Marta AU - Wojcik M AD - Department of General and Minimally Invasive Surgery, Franciszek Raszeja Hospital, Poznan, Poland. FAU - Romanczuk, Michal AU - Romanczuk M AD - Department of General Surgery, Hospital in Siedlce, Siedlce, Poland. FAU - Mitura, Bernard AU - Mitura B AD - Jagiellonian University Collegium Medicum, Cracow, Poland. FAU - Sawicka, Malgorzata AU - Sawicka M AD - Department of General Surgery, Hospital in Siedlce, Siedlce, Poland. FAU - Golyski, Jakub AU - Golyski J AD - Department of General and Oncological Surgery, St. John of Dukla Lublin Region Oncology Center, Lublin, Poland. FAU - Niecikowski, Piotr AU - Niecikowski P AD - Faculty of Medical and Health Sciences, University of Siedlce, Siedlce, Poland. AD - Department of General Surgery, Hospital in Siedlce, Siedlce, Poland. LA - eng PT - Journal Article DEP - 20260731 PL - England TA - BMC Surg JT - BMC surgery JID - 100968567 SB - IM MH - Humans MH - Feasibility Studies MH - *Hernia, Inguinal/surgery MH - Prospective Studies MH - Female MH - *Herniorrhaphy/methods MH - Middle Aged MH - Male MH - Aged MH - *Surgical Mesh MH - *Laparoscopy/methods MH - Adult MH - *Peritoneum/surgery MH - Aged, 80 and over PMC - PMC13715205 OTO - NOTNLM OT - Inguinal hernia OT - Laparoscopic surgery OT - Mesh positioning OT - Preperitoneal desufflation OT - TAPP repair COIS- Declarations. Ethics approval and consent to participate: The study was approved by the Ethics Committee of the University of Siedlce, Poland. The study was conducted in accordance with the Declaration of Helsinki. Written informed consent was obtained from all individual participants included in the study. Consent for publication: Not applicable. Competing interests: Prof. Kryspin Mitura has received honoraria for lectures and educational activities from Medtronic, Olympus, TZMO, B. Braun, Corza Medical and BD (Bard). Laura Kacprzak, Marta Wojcik, Michal Romanczuk, Bernard Mitura, Malgorzata Sawicka, Krystian Kisielewski, Jakub Golynski and Piotr Niecikowski declare that they have no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:38 PHST- 2026/02/04 00:00 [received] PHST- 2026/07/29 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:38 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s12893-026-04104-7 [pii] AID - 4104 [pii] AID - 10.1186/s12893-026-04104-7 [doi] PST - epublish SO - BMC Surg. 2026 Jul 31;26(1):724. doi: 10.1186/s12893-026-04104-7. PMID- 42947075 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1471-2415 (Electronic) IS - 1471-2415 (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Orbital involvement by spindle cell squamous cell carcinoma: a case report of a rare and aggressive tumor with diagnostic challenges. LID - 10.1186/s12886-026-05166-0 [doi] LID - 690 AB - BACKGROUND: Spindle cell squamous cell carcinoma (SCSCC) is a rare and poorly differentiated variant of squamous cell carcinoma that may mimic sarcoma, melanoma, and other spindle cell malignancies. Orbital involvement is exceptionally uncommon and may pose substantial diagnostic and therapeutic challenges, particularly when overt squamous differentiation is absent. CASE PRESENTATION: A 60-year-old woman with a history of left ocular evisceration for a blind painful eye presented six months later with rapidly progressive painful swelling of the left lower eyelid and medial canthus. Orbital magnetic resonance imaging demonstrated an infiltrative, heterogeneously enhancing orbital mass, and positron emission tomography-computed tomography showed hypermetabolic uptake in the orbital lesion with additional ipsilateral cervical and parotid-region foci. The patient underwent orbital exenteration combined with radical neck dissection and radical parotidectomy. Histopathologic examination revealed a malignant spindle cell neoplasm with marked pleomorphism and frequent mitotic activity, without definite keratinization or overt squamous differentiation. Immunohistochemical staining showed strong positivity for pancytokeratin AE1/AE3, CK OSCAR, and CK8/18, supporting epithelial differentiation and a diagnosis most consistent with spindle cell squamous cell carcinoma. Despite adjuvant radiotherapy and systemic chemotherapy, metastatic disease developed within three months, and the patient died six months after orbital exenteration. CONCLUSIONS: This case highlights the diagnostic difficulty and aggressive clinical behavior of orbital spindle cell malignancies with features most consistent with spindle cell squamous cell carcinoma. Broad immunohistochemical evaluation, careful clinicopathological correlation, comprehensive staging, and early multidisciplinary management are essential in such rare and high-risk tumors. CI - (c) 2026. The Author(s). FAU - Oyur, Gulistan AU - Oyur G AUID- ORCID: 0000-0002-5780-7898 AD - Department of Ophthalmology, Basaksehir Cam and Sakura City Hospital, Istanbul, Turkey. gulistanoyur@hotmail.com. FAU - Izol, Hazal AU - Izol H AD - Department of Pathology, Basaksehir Cam and Sakura City Hospital, Istanbul, Turkey. FAU - Savur, Fatma AU - Savur F AD - Department of Ophthalmology, Basaksehir Cam and Sakura City Hospital, Istanbul, Turkey. LA - eng PT - Case Reports PT - Journal Article DEP - 20260731 PL - England TA - BMC Ophthalmol JT - BMC ophthalmology JID - 100967802 SB - IM MH - Humans MH - Female MH - Middle Aged MH - *Carcinoma, Squamous Cell/diagnosis MH - *Orbital Neoplasms/diagnosis MH - Magnetic Resonance Imaging MH - Diagnosis, Differential MH - Cutaneous Squamous Cell Carcinoma MH - Positron Emission Tomography Computed Tomography PMC - PMC13711776 OTO - NOTNLM OT - Case report OT - Immunohistochemistry OT - Orbital exenteration OT - Orbital invasion OT - Spindle cell squamous cell carcinoma COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Full written informed consent for publication was obtained from patients and /or their guardians. Patient consent: Written informed consent for publication of clinical details and images was obtained from the patient. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:38 PHST- 2026/03/14 00:00 [received] PHST- 2026/07/23 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:38 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s12886-026-05166-0 [pii] AID - 5166 [pii] AID - 10.1186/s12886-026-05166-0 [doi] PST - epublish SO - BMC Ophthalmol. 2026 Jul 31;26(1):690. doi: 10.1186/s12886-026-05166-0. PMID- 42947071 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1471-244X (Electronic) IS - 1471-244X (Linking) VI - 26 IP - 1 DP - 2026 Oct 11 TI - Interpretable machine learning for identifying mild cognitive impairment among Chinese patients with obstructive sleep apnea: a multicenter cross-sectional study. LID - 10.1186/s12888-026-08712-8 [doi] LID - 833 AB - BACKGROUND: Mild cognitive impairment (MCI) is clinically relevant in obstructive sleep apnea (OSA). We developed and externally validated an interpretable machine-learning model for concurrent clinically defined MCI to support prioritization for formal cognitive assessment rather than diagnosis. METHODS: This multicenter cross-sectional diagnostic-prediction study included 400 eligible patients with OSA from Changchun, divided into a training set (n = 280) and an internal algorithm-selection hold-out (n = 120), plus an independent Shenzhen external-validation sample (n = 192). MCI classification integrated reported cognitive decline, objective cognitive evidence, largely preserved everyday independence, dementia exclusion, and assessment-team consensus with physician participation. Of 27 candidate predictors, redundancy review left 24 and LASSO-Boruta consensus retained nine. Feature selection was performed once on the full training set before repeated cross-validation and was not nested within resampling folds. Eight algorithms were tuned using repeated 10-fold cross-validation with training-fold-only SMOTE and PR-AUC as the primary metric. The RF threshold (0.3228) was derived from training out-of-fold probabilities and locked before validation. RESULTS: MCI was classified in 93/280 (33.2%), 38/120 (31.7%), and 57/192 (29.7%) participants. The nine RF predictors were coffee consumption, physical activity, AHI, MSaO2, PHQ-9, GAD-7, ESS, CPSS-14, and PSQI total scores. In the internal hold-out used for algorithm selection, RF achieved ROC-AUC 0.953, PR-AUC 0.936, and Brier score 0.060; these selection-conditioned estimates are not unbiased generalization estimates. In independent external validation, ROC-AUC was 0.957, PR-AUC 0.809, and Brier score 0.051. Calibration intercept - 0.616 and slope 0.701 indicated systematic probability overestimation despite strong discrimination. CONCLUSIONS: The RF model discriminated strongly in Shenzhen, but external miscalibration and systematic probability overestimation mean that high AUC does not make raw probabilities transportable; local calibration assessment and, if needed, recalibration are required before clinical use. The MCI outcome cannot be reproduced from questionnaire/scale scores alone, and Supplementary Table S10 is a retrospective operational specification rather than the original CRF. Performance evidence comes almost entirely from male OSA patients; validity in women is unestablished, and the model should not be used for clinical assessment in females. It is a research screening-prioritization tool, not a diagnostic, causal, prognostic, or clinical-impact instrument. CI - (c) 2026. The Author(s). FAU - Li, Enguang AU - Li E AD - College of Management, Changchun University of Chinese Medicine, No. 1035 Boshuo Road, Changchun Jingyue National High-tech Industrial Development Zone, Changchun, Jilin Province , 130117, China. FAU - Ai, Fangzhu AU - Ai F AD - Liaoning University of Traditional Chinese Medicine, No. 79 Chongshan East Road, Huanggu District, Shenyang, Liaoning Province, 110847, China. FAU - Cai, Peipei AU - Cai P AD - Department of Accounting and Finance, Heilongjiang Polytechnic, No. 5 Xuefu Road, Nangang District, Harbin, Heilongjiang Province, 150080, China. FAU - Wen, Kuo AU - Wen K AD - College of Traditional Chinese Medicine, Changchun University of Chinese Medicine, No. 1035 Boshuo Road, Jingyue National High-tech Industrial Development Zone, Changchun, Jilin Province, 130117, China. FAU - Guo, Botang AU - Guo B AD - Psychology and Health Management Center, Harbin Medical University, No. 157 Baojian Road, Nangang District, Harbin, Heilongjiang Province, 150078, China. hmugbt@hrbmu.edu.cn. AD - Department of General Practice, The Affiliated Luohu Hospital of Shenzhen University Medical School, No. 47 Youyi Road, Luohu District, Shenzhen, Guangdong Province , 518005, China. hmugbt@hrbmu.edu.cn. FAU - Wen, Hongjuan AU - Wen H AUID- ORCID: 0009-0007-0265-1690 AD - College of Management, Changchun University of Chinese Medicine, No. 1035 Boshuo Road, Changchun Jingyue National High-tech Industrial Development Zone, Changchun, Jilin Province , 130117, China. wenhongjuan2004@163.com. LA - eng GR - 82607167/National Natural Science Foundation of China/ GR - 2024260/Science and Technology Project of the Jilin Provincial Administration of Traditional Chinese Medicine/ GR - 2024YJ03/Changchun University of Traditional Chinese Medicine Theme Case Project/ GR - ZT-2510199001/2025 Thematic Case Project of the Development Center for Degree and Graduate Education, Ministry of Education/ PT - Journal Article PT - Multicenter Study DEP - 20261011 PL - England TA - BMC Psychiatry JT - BMC psychiatry JID - 100968559 RN - Chinese people SB - IM MH - Humans MH - *Sleep Apnea, Obstructive/complications MH - Cross-Sectional Studies MH - Female MH - *Cognitive Dysfunction/diagnosis/complications MH - *Machine Learning MH - Male MH - Aged MH - China MH - Middle Aged MH - Algorithms MH - Classification Algorithms MH - Prediction Algorithms MH - East Asian People PMC - PMC13712065 OTO - NOTNLM OT - Chinese population OT - Clinical prediction model OT - Machine learning OT - Mild cognitive impairment OT - Obstructive sleep apnea OT - SHAP OT - TRIPOD + AI COIS- Declarations. Ethics approval and consent to participate: Ethics approval was granted by the Ethics Committee of the Affiliated Hospital of Changchun University of Chinese Medicine (CCZYFYLL-SQ-2024-227) and the Shenzhen Luohu People's Hospital Research Ethics Committee for the external-center study (2024-LHQRMYY-KYLL-071). The Shenzhen committee approved the study protocol and written informed-consent form. All procedures complied with the Declaration of Helsinki and its later amendments, and written informed consent was obtained from all participants. Consent for publication: Not applicable. Study registration and protocol availability: This observational cross-sectional study was not prospectively registered. Analysis and implementation code supporting the reported workflow and the versioned reproducibility materials have been deposited in the public GitHub repository and versioned Zenodo record described above; this retrospective archiving should not be interpreted as prospective registration or as evidence of a prospectively registered protocol. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/11 CRDT- 2026/10/28 00:37 PHST- 2026/06/06 00:00 [received] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:37 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - 10.1186/s12888-026-08712-8 [pii] AID - 8712 [pii] AID - 10.1186/s12888-026-08712-8 [doi] PST - epublish SO - BMC Psychiatry. 2026 Oct 11;26(1):833. doi: 10.1186/s12888-026-08712-8. PMID- 42947065 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1471-2377 (Electronic) IS - 1471-2377 (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Association of jugular venous oxygen saturation and mean arterial pressure variability with dynamic cerebral autoregulation and outcomes in sepsis-associated encephalopathy. LID - 10.1186/s12883-026-05203-x [doi] LID - 674 AB - BACKGROUND: Sepsis-associated encephalopathy (SAE) is a frequent and clinically important manifestation of sepsis-related acute brain dysfunction. Impaired cerebral autoregulation and altered cerebral oxygen supply-demand balance are considered relevant pathophysiological features, but the temporally aligned and statistically adjusted relationships among jugular venous oxygen saturation (SjvO2), mean arterial pressure (MAP) variability, and dynamic cerebral autoregulation (dCA) remain insufficiently defined. OBJECTIVE: This retrospective study examined the associations between SjvO2, MAP variability, and dCA indices in neurocritical care patients with SAE and evaluated whether these monitoring variables were associated with impaired dCA and short-term outcomes. METHODS: We retrospectively analyzed 200 adult neurocritical care and intensive care patients with SAE selected from an indication-based advanced neuromonitoring registry between January 2022 and December 2025. Jugular bulb catheterization was performed for clinical indications rather than as universal screening. SjvO2 monitoring, invasive arterial pressure monitoring, and transcranial Doppler-based dCA assessment were matched within the same early neuromonitoring window after SAE recognition. MAP variability was quantified using the coefficient of variation (MAP-CV) over the 24-hour period containing the first valid dCA session, and SjvO2 was derived from the matched 60-minute monitoring epoch rather than pooled ICU-stay data. Dynamic cerebral autoregulation was assessed by transfer function analysis and the mean flow index (Mx). Multivariable logistic and Cox regression models were adjusted for prespecified illness-severity and physiological covariates. RESULTS: The mean SjvO2 was 62.4 +/- 11.8%, with 34.5% of patients demonstrating abnormal values (< 55% or > 75%). MAP-CV was 14.2 +/- 5.6%, and impaired dCA (Mx > 0.3) was observed in 58.0% of patients. SjvO2 was negatively correlated with Mx (r=-0.487, p < 0.001) and positively correlated with phase difference (r = 0.412, p < 0.001). MAP-CV was positively correlated with Mx (r = 0.523, p < 0.001) and negatively correlated with phase difference (r=-0.394, p < 0.001). Multivariable analysis identified SjvO2 < 55% (OR = 3.24, 95%CI: 1.78-5.89, p < 0.001), MAP-CV > 15% (OR = 2.87, 95%CI: 1.56-5.28, p = 0.001), and SOFA score (OR = 1.18, 95%CI: 1.06-1.32, p = 0.003) as independent predictors of impaired dCA. Patients with both abnormal SjvO2 and high MAP-CV had higher 28-day mortality than those with normal SjvO2 and low/moderate MAP-CV (51.0% vs. 22.1%, p < 0.001). In a simultaneous Cox model including impaired dCA, the combined abnormal SjvO2/high MAP-CV phenotype remained associated with 28-day mortality (adjusted HR = 2.54, 95%CI: 1.42-4.55, p = 0.002). CONCLUSIONS: Lower SjvO2 and greater MAP variability were associated with impaired dCA in SAE patients. The combined assessment of cerebral oxygenation and MAP variability may help identify patients with higher neurological and mortality risk within an observational risk-stratification framework. These findings are hypothesis-generating and require prospective validation before being used to define individualized hemodynamic targets or treatment effects. CI - (c) 2026. The Author(s). FAU - Wang, Zhenyu AU - Wang Z AD - Department of Neurology, Affiliated Hospital of Hubei, Xiangyang Central Hospital, University of Arts and Science, Xiangyang, Hubei, China. FAU - Yu, Heng AU - Yu H AD - Department of Neurology, Affiliated Hospital of Hubei, Xiangyang Central Hospital, University of Arts and Science, Xiangyang, Hubei, China. FAU - Yang, Liu AU - Yang L AD - Department of Neurology, Affiliated Hospital of Hubei, Xiangyang Central Hospital, University of Arts and Science, Xiangyang, Hubei, China. 17351378351@163.com. FAU - Cui, Yonghe AU - Cui Y AUID- ORCID: 0009-0006-4575-5097 AD - Department of Critical Care Medicine, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, Hubei, China. yonghecui@foxmail.com. LA - eng PT - Journal Article DEP - 20260731 PL - England TA - BMC Neurol JT - BMC neurology JID - 100968555 RN - S88TT14065 (Oxygen) SB - IM MH - Humans MH - *Homeostasis/physiology MH - *Arterial Pressure/physiology MH - Retrospective Studies MH - Male MH - Female MH - *Jugular Veins/metabolism MH - *Cerebrovascular Circulation/physiology MH - *Sepsis-Associated Encephalopathy/physiopathology MH - *Oxygen Saturation/physiology MH - Middle Aged MH - Aged MH - *Oxygen/blood PMC - PMC13715285 OTO - NOTNLM OT - Dynamic cerebral autoregulation OT - Jugular venous oxygen saturation OT - Mean arterial pressure variability OT - Neurocritical care OT - Retrospective cohort OT - Sepsis-associated encephalopathy OT - Transcranial Doppler COIS- Declarations. Ethics approval and consent to participate: The study was submitted to and approved by the Ethics Committee of Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science. The study was conducted in accordance with the Declaration of Helsinki. Because this was a retrospective analysis of anonymized clinical data generated during routine care, and because jugular bulb catheterization, invasive arterial pressure monitoring, and transcranial Doppler ultrasonography were performed for clinical indications, the requirement for individual informed consent was waived by the ethics committee. No separate ethics approval reference number was issued for this retrospective consent-waiver review. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:37 PHST- 2026/02/20 00:00 [received] PHST- 2026/07/20 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:37 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s12883-026-05203-x [pii] AID - 5203 [pii] AID - 10.1186/s12883-026-05203-x [doi] PST - epublish SO - BMC Neurol. 2026 Jul 31;26(1):674. doi: 10.1186/s12883-026-05203-x. PMID- 42947059 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1471-2334 (Electronic) IS - 1471-2334 (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Prevalence of hepatitis B virus infection and associated factors among pregnant women attending antenatal care in public hospitals of the Eastern Zone of Tigray, Northern Ethiopia: a cross-sectional study. LID - 10.1186/s12879-026-14141-5 [doi] LID - 1896 AB - BACKGROUND: Hepatitis B virus (HBV) infection remains a major global public health challenge, particularly in sub-Saharan Africa, where transmission occurs through exposure to infected blood and body fluids, sexual contact, and mother-to-child transmission. Chronic HBV infection can result in cirrhosis, hepatocellular carcinoma, and premature mortality. Evidence on the prevalence of HBV infection and its associated factors among pregnant women in Ethiopia, particularly in the Eastern Zone of Tigray, remains limited. This study assessed the prevalence of HBV infection and associated factors among pregnant women attending antenatal care (ANC) services in public hospitals in the Eastern Zone of Tigray, Northern Ethiopia. METHODS: An institution-based cross-sectional study was conducted among 536 pregnant women attending antenatal care in four public hospitals in Eastern Tigray, Ethiopia, from January 1 to March 31, 2025. Hospitals and study participants were selected using simple random and systematic sampling techniques, respectively. Data were collected using a structured interviewer-administered questionnaire. Hepatitis B virus infection status was determined using a single rapid hepatitis B surface antigen (HBsAg) test (ABON Rapid Test), without ELISA or molecular (HBV DNA) confirmation. Data were analyzed using SPSS version 24. Bivariate and multivariable logistic regression analyses were performed to identify factors associated with HBV infection. Statistical significance was declared at p < 0.05. RESULTS: All 536 eligible pregnant women participated in the study, yielding a response rate of 100%. The mean age of participants was 27.9 +/- 5.9 years (range: 16-48 years). The prevalence of HBV infection was 7.8%. Factors significantly associated with HBV infection were women aged >/= 30 years (AOR = 2.72; 95% CI: 1.21-6.10), a history of multiple sexual partners (AOR = 16.12; 95% CI: 5.26-49.47), a history of hospital admission (AOR = 3.24; 95% CI: 1.92-15.32), and alcohol consumption (AOR = 5.42; 95% CI: 1.92-15.34). CONCLUSION: The prevalence of HBV infection among pregnant women attending ANC services in public hospitals of the Eastern Zone of Tigray was 7.8%, indicating a high-intermediate level of endemicity. HBV infection remains a public health concern among pregnant women in the study area. Strengthening routine antenatal HBV screening and appropriate linkage to care and vaccination, enhancing infection prevention measures, and providing targeted health education may be recommended to reduce the burden of HBV infection among pregnant women and their infants. CI - (c) 2026. The Author(s). FAU - Gebretsadik, Alem AU - Gebretsadik A AD - Department of Nutrition and Health Systems, School of Public Health, College of Medicine and Health Sciences, Adigrat University, Adigrat, Tigray, Ethiopia. aglab2006@gmail.com. FAU - Gebreegziabiher, Gebremedhin AU - Gebreegziabiher G AD - Department of Nutrition and Health Systems, School of Public Health, College of Medicine and Health Sciences, Adigrat University, Adigrat, Tigray, Ethiopia. LA - eng PT - Journal Article DEP - 20260731 PL - England TA - BMC Infect Dis JT - BMC infectious diseases JID - 100968551 RN - 0 (Hepatitis B Surface Antigens) SB - IM MH - Humans MH - Female MH - Ethiopia/epidemiology MH - Pregnancy MH - Cross-Sectional Studies MH - Adult MH - Hospitals, Public MH - Young Adult MH - *Prenatal Care MH - *Hepatitis B/epidemiology MH - Prevalence MH - *Pregnancy Complications, Infectious/epidemiology/virology MH - Adolescent MH - Risk Factors MH - Hepatitis B Surface Antigens/blood MH - Hepatitis B virus PMC - PMC13715142 OTO - NOTNLM OT - Antenatal care OT - Associated factors OT - Cross-sectional studies OT - Ethiopia OT - Hepatitis B virus OT - Pregnant women OT - Prevalence COIS- Declarations. Ethics approval and consent to participate: Ethical approval was obtained from the Research Ethics Review Committee of the College of Medicine and Health Sciences, Adigrat University (Ref. No. ADU/CMHS/034/2024; 15/10/2024). Permission was obtained from the participating public hospitals. Written informed consent was obtained from all participants aged 18 years and above. For pregnant participants aged 16-17 years, written assent was obtained from the participant, and written informed consent was obtained from a parent or legal guardian before enrollment. Participation was voluntary, and confidentiality was maintained using unique identification codes. HBsAg-positive women received counseling and referral for appropriate care according to national guidelines. The study was conducted in accordance with the ethical principles of the Declaration of Helsinki. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:37 PHST- 2025/12/21 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:37 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s12879-026-14141-5 [pii] AID - 14141 [pii] AID - 10.1186/s12879-026-14141-5 [doi] PST - epublish SO - BMC Infect Dis. 2026 Jul 31;26(1):1896. doi: 10.1186/s12879-026-14141-5. PMID- 42947054 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1471-2342 (Electronic) IS - 1471-2342 (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Gliomas grading prediction using multicenter 3T Magnetic Resonance Spectroscopy. LID - 10.1186/s12880-026-02640-5 [doi] LID - 559 AB - BACKGROUND: Magnetic Resonance Spectroscopy (MRS) is a non-invasive imaging technique that detects various metabolites in brain tissues, making it essential for the grading of brain gliomas. MRS measures key metabolites such as N-acetyl-aspartate (NAA), choline (Cho), and creatine (Cr), whose ratio changes provide valuable information for glioma grading, guiding diagnosis, treatment, and prognosis. Current studies are largely single-center, with grading criteria differing across hospitals. This work aims to identify reliable and consistent metabolite ratios for glioma grading by jointly analyzing multicenter 3T single-voxel MRS of brain gliomas. The grading accuracy of these metabolite ratios is evaluated to establish a more standardized diagnostic basis. MATERIALS AND METHODS: We retrospectively included the MRS data from 60 glioma patients from 3 hospitals. MRS results included 4 metabolite ratios: NAA/Cr, Cho/NAA, Cho/Cr, and NAA/(Cho + Cr). The Shapiro-Wilk test was employed to ascertain the normality of the data, and the differences were calculated using either an independent t-test or a Mann-Whitney U-test. The classification performance was evaluated using ROC curve and accuracy analysis. RESULTS: Across multicenter data, statistically significant differences were found in Cho/NAA and Cho/Cr ratios between low- and high-grade gliomas (P < 0.05). ROC analysis showed AUCs of 0.73 for Cho/NAA and 0.74 for Cho/Cr, indicating good classification performance. Validation results showed 75% accuracy, supporting their potential for clinical glioma grading. The optimal cut-off value trained from any two hospitals is validated at a third hospital, and the accuracies of Cho/Cr are 77.78%, 100%, and 65%, respectively. CONCLUSION: Multicenter data analysis confirmed the good classification accuracy of Cho/NAA and Cho/Cr in glioma grading, providing a reliable basis for clinical treatment and prognosis planning. This aligns with findings from other studies. CLINICAL TRIAL NUMBER: Not applicable. CI - (c) 2026. The Author(s). FAU - Guo, Di AU - Guo D AD - School of Computer and Information Engineering, Fujian Engineering Research Center for Medical Data Mining and Application, Xiamen University of Technology, Xiamen, 361024, China. FAU - Xie, Kaiwei AU - Xie K AD - School of Computer and Information Engineering, Fujian Engineering Research Center for Medical Data Mining and Application, Xiamen University of Technology, Xiamen, 361024, China. FAU - Lin, Jiacheng AU - Lin J AD - School of Computer and Information Engineering, Fujian Engineering Research Center for Medical Data Mining and Application, Xiamen University of Technology, Xiamen, 361024, China. FAU - Chen, Jianshu AU - Chen J AD - Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, School of Electronic Science and Engineering, Xiamen University, Xiamen, 361005, China. FAU - Zhang, Jialue AU - Zhang J AD - Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, School of Electronic Science and Engineering, Xiamen University, Xiamen, 361005, China. FAU - Lin, Meijin AU - Lin M AD - Department of Applied Marine Physics and Engineering, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, Xiamen University, Xiamen, 361005, China. FAU - Qu, Xiaobo AU - Qu X AD - Department of Electronic Science, Fujian Provincial Key Laboratory of Plasma and Magnetic Resonance, School of Electronic Science and Engineering, Xiamen University, Xiamen, 361005, China. AD - Department of Radiology, The First Affiliated Hospital of Xiamen University, 55 Zhenhai Road, Siming District, Xiamen, 361000, China. FAU - Hu, Wanjun AU - Hu W AD - Department of Nuclear Magnetic Resonance, The Second Hospital & Clinical Medical School, Gansu Province Clinical Research Center for Functional and Molecular Imaging, Lanzhou University, Lanzhou, 730030, China. huwj2023@lzu.edu.cn. FAU - Wang, Jianhua AU - Wang J AD - Department of Radiology, The First Affiliated Hospital of Xiamen University, 55 Zhenhai Road, Siming District, Xiamen, 361000, China. wangjianhua@xmu.edu.cn. FAU - Liang, Dongyun AU - Liang D AD - Department of Radiology, Zhongshan Hospital (Xiamen), Xiamen Municipal Clinical Research Center for Medical Imaging, Fujian Province Key Clinical Specialty for Medical Imaging, Xiamen Key Laboratory of Clinical Transformation of Imaging Big Data and Artificial Intelligence, Fudan University, Xiamen, 361015, China. liang.dongyun@zsxmhospital.com. LA - eng GR - 62371410/National Natural Science Foundation of China/ GR - 61871341/the National Natural Science Foundation of China/ GR - 2023J02005/the Natural Science Foundation of Fujian Province of China/ GR - 231107173160805/Industry-University Cooperation Projects of the Ministry of Education of China/ GR - 21JR11RA122/the Science and Technology Plan Project of Gansu Province/ GR - CY2022-QN-B09/the Cuiying Science and Technology Innovation Program/ GR - 2024GZL-ZD09/the Science and Technology Plan of Major Research Projects of Xiamen High Quality Development/ GR - 3502Z20244ZD2017/Xiamen Medical Engineering Guiding Program/ PT - Journal Article PT - Multicenter Study DEP - 20260731 PL - England TA - BMC Med Imaging JT - BMC medical imaging JID - 100968553 RN - N91BDP6H0X (Choline) RN - 30KYC7MIAI (Aspartic Acid) RN - 997-55-7 (N-acetylaspartate) RN - MU72812GK0 (Creatine) SB - IM MH - Humans MH - *Glioma/pathology/diagnostic imaging/metabolism MH - *Brain Neoplasms/pathology/diagnostic imaging/metabolism MH - Magnetic Resonance Spectroscopy/methods MH - Female MH - Neoplasm Grading MH - Male MH - Choline/metabolism MH - Retrospective Studies MH - Aspartic Acid/analogs & derivatives/metabolism MH - Middle Aged MH - Adult MH - Creatine/metabolism MH - Aged PMC - PMC13715249 OTO - NOTNLM OT - Glioma grading OT - Magnetic Resonance Spectroscopy OT - Metabolites OT - Multicenter study COIS- Declarations. Ethics approval and consent to participate: All data were performed in compliance with relevant laws and institutional guidelines and were approved by the Medical Ethics Committees of Zhongshan Hospital of Fudan University (Xiamen Branch), the Second Hospital of Lanzhou University, and the First Affiliated Hospital of Xiamen University (approval number: B2024-094, 2024 A-181, 2024 - 193, respectively). Furthermore, the Medical Ethics Committees of the aforementioned institutions obtained approval for a waiver of informed consent from patients. Consent for publication: Not applicable. Competing interests: Xiaobo Qu is the senior editor of BMC Medical Imaging. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:36 PHST- 2024/11/19 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:36 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s12880-026-02640-5 [pii] AID - 2640 [pii] AID - 10.1186/s12880-026-02640-5 [doi] PST - epublish SO - BMC Med Imaging. 2026 Jul 31;26(1):559. doi: 10.1186/s12880-026-02640-5. PMID- 42947051 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1471-2407 (Electronic) IS - 1471-2407 (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Metabolic health at breast cancer diagnosis and two-year survival: a prospective cohort study in a public healthcare setting. LID - 10.1186/s12885-026-16532-y [doi] LID - 1309 AB - BACKGROUND: Metabolic syndrome is common among women with breast cancer and may adversely affect prognosis. However, prospective evidence on changes in metabolic health after breast cancer diagnosis and their association with survival remains limited, particularly in routine public healthcare settings. This study evaluated metabolic health during the first two years after diagnosis and examined the association between metabolic syndrome at diagnosis and overall survival in women with breast cancer. METHODS: This prospective cohort study was conducted in a Brazilian public healthcare service and included women aged >/= 40 years with newly diagnosed stage I-III invasive breast carcinoma who were followed for two years. Clinical, anthropometric, and laboratory assessments were performed at baseline and at 6, 12, 18, and 24 months. Metabolic syndrome was defined according to the National Cholesterol Education Program Adult Treatment Panel III criteria. Overall survival was defined as the time from breast cancer diagnosis to death from any cause or last follow-up. Survival according to metabolic syndrome status at diagnosis was evaluated using Kaplan-Meier analysis and Cox proportional hazards models. RESULTS: A total of 165 women were recruited, of whom 126 were included in the analysis and 111 completed the two-year assessment. Among participants with longitudinal data, significant reductions were observed in the prevalence of dysglycemia, elevated systolic blood pressure, and low HDL cholesterol. No significant changes were found in obesity, increased waist circumference, hypertriglyceridemia, or overall metabolic syndrome prevalence. During follow-up, 15 patients died. Women who died had a higher prevalence of metabolic syndrome at diagnosis than survivors (73% vs. 41%, p = 0.020) and higher baseline fasting glucose levels (p = 0.021). Metabolic syndrome at diagnosis was associated with lower overall survival (log-rank p = 0.021) and a higher risk of mortality (hazard ratio 9.09; 95% confidence interval 1.73-47.9), although this estimate should be interpreted as exploratory because of the limited number of events. CONCLUSIONS: In this prospective cohort of women with breast cancer treated in a Brazilian public healthcare setting, metabolic syndrome was highly prevalent at diagnosis and was associated with poorer two-year overall survival in an exploratory analysis. Although favorable changes were observed in selected metabolic parameters during follow-up, obesity and the overall prevalence of metabolic syndrome remained largely unchanged. These findings support the incorporation of metabolic assessment and multidisciplinary cardiometabolic care into breast cancer management and survivorship programs and require confirmation in larger cohorts with longer follow-up. CI - (c) 2026. The Author(s). FAU - Prado, Vanildo AU - Prado V AD - Graduate Program in Tocogynecology, Botucatu Medical School, Sao Paulo State University - UNESP, Botucatu, Sao Paulo, Brazil. FAU - Esperanca, Giuliano AU - Esperanca G AD - Graduate Program in Tocogynecology, Botucatu Medical School, Sao Paulo State University - UNESP, Botucatu, Sao Paulo, Brazil. FAU - Nahas, Georgia Petri AU - Nahas GP AD - Graduate Program in Tocogynecology, Botucatu Medical School, Sao Paulo State University - UNESP, Botucatu, Sao Paulo, Brazil. FAU - Maya, Barbara Gomes AU - Maya BG AD - Graduate Program in Tocogynecology, Botucatu Medical School, Sao Paulo State University - UNESP, Botucatu, Sao Paulo, Brazil. FAU - Rizzi, Samantha AU - Rizzi S AD - Graduate Program in Tocogynecology, Botucatu Medical School, Sao Paulo State University - UNESP, Botucatu, Sao Paulo, Brazil. FAU - Buttros, Daniel AU - Buttros D AD - Graduate Program in Tocogynecology, Botucatu Medical School, Sao Paulo State University - UNESP, Botucatu, Sao Paulo, Brazil. FAU - Nahas, Eliana Aguiar Petri AU - Nahas EAP AD - Graduate Program in Tocogynecology, Botucatu Medical School, Sao Paulo State University - UNESP, Botucatu, Sao Paulo, Brazil. eliana.nahas@unesp.br. AD - Department of Gynecology and Obstetrics, Botucatu Medical School, Sao Paulo State University - UNESP, Botucatu, SP, 18618-970, Brazil. eliana.nahas@unesp.br. LA - eng PT - Journal Article DEP - 20260731 PL - England TA - BMC Cancer JT - BMC cancer JID - 100967800 SB - IM MH - Humans MH - Female MH - *Breast Neoplasms/mortality/metabolism/diagnosis/complications/epidemiology MH - *Metabolic Syndrome/epidemiology MH - Middle Aged MH - Prospective Studies MH - Brazil/epidemiology MH - Adult MH - Aged MH - Prognosis MH - Kaplan-Meier Estimate PMC - PMC13711900 OTO - NOTNLM OT - Breast cancer OT - Cardiometabolic health OT - Metabolic syndrome OT - Prospective cohort OT - Public healthcare OT - Survival COIS- Declarations. Ethics approval and consent to participate: The study was approved by the Research Ethics Committee of the Botucatu Medical School - UNESP (approval number: 3.493.971, CAAE: 1574019.8.0000.5411), and conducted in accordance with the Declaration of Helsinki. Written informed consent was obtained from all participants before enrollment. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:36 PHST- 2026/04/26 00:00 [received] PHST- 2026/07/07 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:36 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s12885-026-16532-y [pii] AID - 16532 [pii] AID - 10.1186/s12885-026-16532-y [doi] PST - epublish SO - BMC Cancer. 2026 Jul 31;26(1):1309. doi: 10.1186/s12885-026-16532-y. PMID- 42947050 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1471-2431 (Electronic) IS - 1471-2431 (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Prevalence of hypocalcemia in neonates receiving phototherapy: a systematic review and meta-analysis. LID - 10.1186/s12887-026-07442-w [doi] LID - 1070 AB - BACKGROUND: Although phototherapy is the standard treatment for neonatal hyperbilirubinemia, its adverse effects cause electrolyte disturbances. Aggregated evidence regarding the prevalence of phototherapy-induced hypocalcemia remains inconsistent across studies. Therefore, this systematic review and meta-analysis aimed to determine the pooled incidence hypocalcemia in neonates receiving phototherapy. METHODS: This systematic review and meta-analysis were conducted in compliance with the 2020 PRISMA guidelines. Relevant databases were searched to retrieve observational studies reporting hypocalcemia among neonates with hyperbilirubinemia through February 2026. The retrieved articles were cleaned for duplicates and selected based on the eligibility criteria. STATA 17.0 software was used to compute the pooled hypocalcemia with 95% confidence intervals via a random effects model. Heterogeneity was assessed via the I(2) statistic and Cochran's Q test, whereas robustness was evaluated via leave-one-out sensitivity analysis and subgroup analysis. Furthermore, publication bias was assessed by Egger's regression test, Begg's rank correlation test, and trim-and-fill correction. RESULTS: In total, 25 studies involving 2673 neonates were included in the meta-analysis. The pooled prevalence of hypocalcemia in neonates undergoing phototherapy was estimated to be 20.68% (95% CI: 15.75-25.60%). There was significant heterogeneity (I(2) = 94.15%, p < 0.001). In the subgroup analysis, there was a greater hypocalcemia in studies with preterm (23.03%, 95% CI: 14.18% - 31.88%) and low birth weight (22.57%, 95% CI: 14.99% - 30.16%) neonates than in those with term (18.51%, 95% CI: 12.39% - 24.63%) and normal birth weights (13.97%, 95% CI: 8.72% - 19.17%). The hypocalcemia was higher in Africa (36.00%, 95% CI: 7.52% - 64.47%) than in Asia (18.89%, 95% CI: 14.02% - 23.76%). Egger's test revealed significant publication bias (p < 0.0001), with no significant change in the original effect size after trim-and-fill analysis. CONCLUSION: Hypocalcemia is a frequent electrolyte disorder in neonates under phototherapy with neonatal hyperbilirubinemia. Thus, regular monitoring is essential, particularly for those who are at high risk. CI - (c) 2026. The Author(s). FAU - Belayhun, Endashaw AU - Belayhun E AD - Department of Neonatal Nursing, Asrat Weldeyes Health Science Campus, Debre Berhan University, Debre Berhan, Ethiopia. endashawbelayhun1@gmail.com. FAU - Feyisa, Mahider Shimelis AU - Feyisa MS AD - School of Medical Laboratory Sciences, Asrat Weldeyes Health Science Campus, Debre Berhan University, Debre Berhan, Ethiopia. FAU - Tefera, Marye Wondimnew AU - Tefera MW AD - School of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia. FAU - Tilahun, Mihret AU - Tilahun M AD - School of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia. FAU - Achame, Mikiyas Shemelis AU - Achame MS AD - School of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia. FAU - Arage, Rahel Birhanu AU - Arage RB AD - Department of Midwifery, Asrat Weldeyes Health Science Campus, Debre Berhan University, Debre Berhan, Ethiopia. FAU - Sebsibe, Saleamlak AU - Sebsibe S AD - School of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia. FAU - Desale, Sisay AU - Desale S AD - School of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia. FAU - Kassa, Sisay AU - Kassa S AD - ALKAN Health Science Business and Technology College, Dessie Campus, Dessie, Ethiopia. FAU - Asmare, Yabsira AU - Asmare Y AD - School of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia. FAU - Erkihun, Yonas AU - Erkihun Y AD - School of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia. FAU - Mulatie, Zewudu AU - Mulatie Z AD - School of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia. FAU - Eshetu, Bruktawit AU - Eshetu B AD - School of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia. FAU - Habtamu, Afewerk AU - Habtamu A AD - School of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia. LA - eng PT - Journal Article PT - Meta-Analysis PT - Systematic Review DEP - 20260731 PL - England TA - BMC Pediatr JT - BMC pediatrics JID - 100967804 SB - IM MH - Humans MH - *Hypocalcemia/epidemiology/etiology MH - *Phototherapy/adverse effects MH - Infant, Newborn MH - *Hyperbilirubinemia, Neonatal/therapy MH - Prevalence PMC - PMC13711736 OTO - NOTNLM OT - Hyperbilirubinemia OT - Hypocalcemia OT - Meta-analysis OT - Neonates OT - Phototherapy COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:36 PHST- 2026/06/18 00:00 [received] PHST- 2026/07/27 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:36 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s12887-026-07442-w [pii] AID - 7442 [pii] AID - 10.1186/s12887-026-07442-w [doi] PST - epublish SO - BMC Pediatr. 2026 Jul 31;26(1):1070. doi: 10.1186/s12887-026-07442-w. PMID- 42947044 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1471-2431 (Electronic) IS - 1471-2431 (Linking) VI - 26 IP - 1 DP - 2026 Oct 26 TI - Gestational age-stratified analysis of HALP score and complete blood count-derived inflammatory indices in retinopathy of prematurity. LID - 10.1186/s12887-026-07786-3 [doi] LID - 1073 AB - BACKGROUND: To evaluate the temporal profile and discriminative performance of the hemoglobin-albumin-lymphocyte-platelet (HALP) score and complete blood count (CBC)-derived inflammatory indices for retinopathy of prematurity (ROP), with analyses stratified by gestational age. METHODS: This retrospective single-center cohort study included 139 preterm infants born at /= 37 weeks of gestation. Ultrasonographic measurements were performed prior to the onset of labor to assess the SPA and OCD. Maternal, fetal, and pelvic parameters were compared between women who had vaginal delivery and those who underwent cesarean delivery due to cephalopelvic disproportion. Additionally, correlations between pelvic measurements and maternal characteristics were assessed. RESULTS: Of the participants, 219 (82%) had vaginal deliveries, while 48 (18%) underwent cesarean section due to cephalopelvic disproportion. Mean SPA (94.88 +/- 7.52 degrees vs. 103.64 +/- 7.27 degrees ; p < 0.001) and OCD (112.38 +/- 8.71 mm vs. 121.29 +/- 6.62 mm; p < 0.001) were significantly smaller in the cesarean group. ROC analysis demonstrated that both the SPA and OCD had good discriminatory ability for distinguishing vaginal delivery from cesarean delivery due to cephalopelvic disproportion (AUC: 0.788 and 0.794, respectively). The optimal cut-off values were 100.24 degrees for SPA (81.3% sensitivity, 61.6% specificity) and 116.71 mm for OCD (81.3% sensitivity, 71.2% specificity). Cesarean delivery rates were significantly higher among patients with SPA and OCD values below these cut-off thresholds. A weak positive correlation was found between SPA and OCD (r = 0.269, p = 0.011), while maternal age, BMI, height, and gestational age showed no significant associations with pelvic measurements. CONCLUSION: A narrower subpubic angle and a shorter obstetric conjugate diameter, as measured by ultrasonography, were associated with cesarean delivery. These findings suggest that ultrasonographic pelvic measurements may provide useful, non-invasive information and may complement conventional obstetric assessment. CI - (c) 2026. The Author(s). FAU - Secen, Elcin Islek AU - Secen EI AUID- ORCID: 0000-0002-0892-8589 AD - Faculty of Medicine, Department of Obstetrics and Gynecology, MD, Ankara Yidirim Beyazit University, Ankara, Turkey. elcinislek@gmail.com. FAU - Toksoz, Raziye AU - Toksoz R AUID- ORCID: 0000-0002-5190-5083 AD - Faculty of Medicine, Department of Obstetrics and Gynecology, MD, Ankara Yidirim Beyazit University, Ankara, Turkey. FAU - Kaya, Mert Ishak AU - Kaya MI AUID- ORCID: 0000-0001-6258-7597 AD - Ankara Bilkent City Hospital, Department of Obstetrics and Gynecology, MD, University of Health Sciences, Ankara, Turkey. FAU - Torun, Melih Emre AU - Torun ME AUID- ORCID: 0009-0006-2004-6710 AD - Ankara Bilkent City Hospital, Department of Obstetrics and Gynecology, MD, University of Health Sciences, Ankara, Turkey. FAU - Ergun, Gonca Turker AU - Ergun GT AUID- ORCID: 0000-0003-1064-8727 AD - Ankara Bilkent City Hospital, Department of Obstetrics and Gynecology, MD, Ankara, Turkey. FAU - Yavuz, Ayse Filiz AU - Yavuz AF AUID- ORCID: 0000-0003-3699-7757 AD - Faculty of Medicine, Department of Obstetrics and Gynecology, MD, Ankara Yidirim Beyazit University, Ankara, Turkey. LA - eng PT - Journal Article DEP - 20261019 PL - England TA - BMC Pregnancy Childbirth JT - BMC pregnancy and childbirth JID - 100967799 SB - IM MH - Humans MH - Female MH - Pregnancy MH - Adult MH - Turkey MH - Prospective Studies MH - *Delivery, Obstetric/methods/statistics & numerical data MH - *Ultrasonography, Prenatal MH - *Cesarean Section/statistics & numerical data MH - *Cephalopelvic Disproportion/diagnostic imaging/surgery MH - ROC Curve MH - Young Adult MH - *Pelvis/diagnostic imaging PMC - PMC13711893 OTO - NOTNLM OT - Cephalopelvic disproportion OT - Pelvimetry OT - Ultrasonography COIS- Declarations. Ethics approval and consent to participate: This study was approved by the Ankara Bilkent City Hospital Clinical Research Ethics Committee (approval number: TABED 1-25-1844). All participants were informed about the study procedures, and written informed consent was obtained from each participant prior to enrollment. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/19 CRDT- 2026/10/28 00:36 PHST- 2025/11/23 00:00 [received] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:36 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - 10.1186/s12884-026-10035-1 [pii] AID - 10035 [pii] AID - 10.1186/s12884-026-10035-1 [doi] PST - epublish SO - BMC Pregnancy Childbirth. 2026 Oct 19;26(1):1229. doi: 10.1186/s12884-026-10035-1. PMID- 42947039 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1471-2415 (Electronic) IS - 1471-2415 (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Evaluation of plasma glucocorticoid receptor beta levels and intraocular pressure elevation during topical dexamethasone use after photorefractive keratectomy. LID - 10.1186/s12886-026-05188-8 [doi] LID - 695 AB - BACKGROUND: This study aimed to investigate the association between plasma glucocorticoid receptor beta (GR beta) levels and steroid-induced ocular hypertension in patients undergoing photorefractive keratectomy (PRK), along with selected autoimmune markers. METHODS: Patients who underwent PRK and used topical dexamethasone were evaluated on the 21st postoperative day. Demographic data, medical history, preoperative and postoperative refractive values, pachymetry, and intraocular pressure (IOP) were recorded. Corrected IOP values after laser treatment were documented. 30 patients with ocular hypertension (Group 1) and 20 with normal IOP (Group 2) were compared for plasma GR beta levels and autoimmune markers including rheumatoid factor (RF), antinuclear antibody (ANA), and anti-double-stranded DNA (anti-dsDNA). Receiver operating characteristic (ROC) analysis and logistic regression analysis were performed to evaluate the discriminatory ability and association of GR beta levels with postoperative ocular hypertension. RESULTS: In Group 1, pre-laser IOP increased from 14.34 +/- 2.64 to 20.10 +/- 5.03 mm Hg post-laser. The mean cIOP, as per pachymetry post-laser, was 27.84 +/- 4.5. Control group mean IOP values before and after laser were 13.42 +/- 2.57 mmHg and 11.81 +/- 2.52 mmHg, respectively. Corrected IOP, according to pachymetry post-laser, was 17.67 +/- 3.14. Pre-laser IOP values did not differ significantly between the groups (p = 0.227), whereas both post-laser IOP and corrected post-laser IOP values were significantly higher in Group 1 compared to controls (p < 0.001 both). RF values were normal in both groups, and ANA/Anti-dsDNA levels showed no significant differences (p = 0.229, p = 0.28 respectively). GR beta levels differed significantly (p = 0.003), with means of 38.32 +/- 8.53 in Group 1 and 66.23 +/- 37.91 in Group 2. ROC analysis showed moderate discriminatory ability of GR beta levels (AUC 0.76), with an optimal cut-off value of 41.39 pg/mL providing 80% sensitivity and 80% specificity. In univariable logistic regression analysis, higher GR beta levels were associated with a lower likelihood of postoperative IOP elevation. CONCLUSION: Plasma GR beta levels may be associated with steroid-induced ocular hypertension in patients using topical steroids. However, these findings should be interpreted cautiously, and larger prospective studies are required to confirm their clinical relevance, establish clinically relevant cut-off values, and determine whether GR beta has predictive value. CI - (c) 2026. The Author(s). FAU - Gerceker Demircan, Sidika AU - Gerceker Demircan S AUID- ORCID: 0009-0000-5289-7292 AD - Ophthalmology Department, Diskapi Yildirim Beyazit Training and Research Hospital, Ankara, Turkey. sdkgrckrdmrcn@hotmail.com. FAU - Yulek, Fatma AU - Yulek F AUID- ORCID: 0000-0002-9473-7665 AD - Ophthalmology Department, Yildirim Beyazit University Ankara Ataturk Training and Research Hospital, Ankara, Turkey. AD - Private Practice, Ophthalmology, Ankara, 06110, Turkey. LA - eng PT - Journal Article DEP - 20260731 PL - England TA - BMC Ophthalmol JT - BMC ophthalmology JID - 100967802 RN - 7S5I7G3JQL (Dexamethasone) RN - 0 (Glucocorticoids) RN - 0 (Receptors, Glucocorticoid) RN - 0 (glucocorticoid receptor beta) RN - 0 (Biomarkers) SB - IM MH - Humans MH - *Dexamethasone/adverse effects/administration & dosage MH - *Photorefractive Keratectomy/adverse effects MH - Female MH - *Intraocular Pressure/drug effects/physiology MH - *Glucocorticoids/adverse effects/administration & dosage MH - *Ocular Hypertension/chemically induced/blood MH - Male MH - Adult MH - *Receptors, Glucocorticoid/blood MH - Administration, Topical MH - Biomarkers/blood MH - ROC Curve MH - Middle Aged MH - Postoperative Complications MH - Young Adult PMC - PMC13715204 OTO - NOTNLM OT - Glucocorticoid receptor beta OT - Ocular hypertension OT - Photorefractive keratectomy OT - Refractive surgery OT - Topical dexamethasone COIS- Declarations. Ethics approval and consent to participate: The study was conducted according to the guidelines of the Declaration of Helsinki and Good Clinical Practice guidelines and the ethical approval for the study has been obtained from the Ethics Committee of Ankara Ataturk Training and Research Hospital (Number: 2011/12/126). Written informed consent for participation was obtained from all participants. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:36 PHST- 2026/01/08 00:00 [received] PHST- 2026/07/28 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:36 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s12886-026-05188-8 [pii] AID - 5188 [pii] AID - 10.1186/s12886-026-05188-8 [doi] PST - epublish SO - BMC Ophthalmol. 2026 Jul 31;26(1):695. doi: 10.1186/s12886-026-05188-8. PMID- 42947038 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1471-2407 (Electronic) IS - 1471-2407 (Linking) VI - 26 IP - 1 DP - 2026 Oct 27 TI - Association of tumor-informed circulating tumor DNA dynamics with radiologic disease assessment in a single-center real-world sarcoma cohort. LID - 10.1186/s12885-026-17180-y [doi] LID - 1311 AB - BACKGROUND: Sarcomas are rare and heterogeneous malignant tumors for which reliable blood-based biomarkers for longitudinal disease monitoring remain limited. Circulating tumor DNA (ctDNA) has emerged as a minimally invasive biomarker in several solid tumors, but its clinical utility in sarcoma remains incompletely defined because of biological heterogeneity, variable ctDNA shedding, and the limited availability of recurrent, panel-coverable genomic alterations across different subtypes. METHODS: We conducted a retrospective real-world study of patients with bone and soft tissue sarcomas treated at a certified high-volume sarcoma center. Patients underwent tumor-normal molecular profiling to identify tumor-informed ctDNA targets. Longitudinal plasma monitoring was performed using a unique molecular identifier (UMI)-based targeted sequencing approach in patients with an identifiable target. ctDNA findings were compared with radiologic assessment, treatment response, and clinical outcomes. In two selected patients with extended longitudinal follow-up, whole-genome-based molecular residual disease (MRD) analysis was performed as an exploratory analysis. RESULTS: Twenty patients were screened, of whom 14 (70%) had an identifiable tumor-informed ctDNA target and were included in the longitudinal analysis. A total of 67 longitudinal plasma samples were analyzed, with a median of 4 samples per patient (range: 2-10). A total of 67 paired liquid biopsy-radiologic assessments were included in the concordance analysis. Concordant assessments were observed in 59 of 67 cases (88.1%), corresponding to substantial agreement between ctDNA and imaging (Cohen's kappa = 0.755; 95% CI, 0.598-0.913). Using radiologic assessment as the reference standard, ctDNA demonstrated a sensitivity of 80.0% and specificity of 94.6% for detecting progressive disease. These assessment-level estimates were descriptive and did not account for repeated measurements within patients. Descriptive review of the longitudinal ctDNA identified patterns consistent with radiologic and clinical disease course in individual patients. In a 3-month landmark analysis, patients with ctDNA-defined progression had numerically shorter progression-free survival than patients without ctDNA-defined progression (9.0 vs. 16.0 months), although the difference was not statistically significant. Exploratory whole-genome MRD analysis was concordant with radiologic disease course in two selected patients. CONCLUSIONS: Tumor-informed ctDNA monitoring was feasible in 70% of screened patients and showed high concordance with radiologic disease assessment in this real-world sarcoma cohort. These findings support prospective evaluation of ctDNA as an adjunct to imaging for longitudinal disease monitoring in patients with a suitable tumor-informed target, while broader tumor-informed approaches may be required for patients whose tumors are not adequately covered by predefined targeted panels. CI - (c) 2026. The Author(s). FAU - Calukovic, Branko AU - Calukovic B AD - Department of Internal Medicine VIII - Medical Oncology and Pneumology, University Hospital Tubingen, Tubingen, Germany. AD - Center for Soft Tissue Sarcomas, GIST and Bone Tumors (ZWS) of the University Hospital Tubingen and the Comprehensive Cancer Center (CCC) Tubingen-Stuttgart, Tubingen, Germany. AD - DFG Cluster of Excellence 2180 'Image-Guided and Functional Instructed Tumor Therapy' (iFIT), University of Tubingen, Tubingen, Germany. FAU - Benzler, Katrin AU - Benzler K AD - Department of Internal Medicine VIII - Medical Oncology and Pneumology, University Hospital Tubingen, Tubingen, Germany. AD - Center for Soft Tissue Sarcomas, GIST and Bone Tumors (ZWS) of the University Hospital Tubingen and the Comprehensive Cancer Center (CCC) Tubingen-Stuttgart, Tubingen, Germany. AD - DFG Cluster of Excellence 2180 'Image-Guided and Functional Instructed Tumor Therapy' (iFIT), University of Tubingen, Tubingen, Germany. FAU - Schulze, Martin AU - Schulze M AD - Center for Human Genetics Tubingen, Tubingen, Germany. FAU - Battke, Florian AU - Battke F AD - CeGaT GmbH, Tubingen, Germany. FAU - Zender, Lars AU - Zender L AD - Department of Internal Medicine VIII - Medical Oncology and Pneumology, University Hospital Tubingen, Tubingen, Germany. AD - Center for Soft Tissue Sarcomas, GIST and Bone Tumors (ZWS) of the University Hospital Tubingen and the Comprehensive Cancer Center (CCC) Tubingen-Stuttgart, Tubingen, Germany. AD - DFG Cluster of Excellence 2180 'Image-Guided and Functional Instructed Tumor Therapy' (iFIT), University of Tubingen, Tubingen, Germany. FAU - Lauer, Ulrich M AU - Lauer UM AD - Department of Internal Medicine VIII - Medical Oncology and Pneumology, University Hospital Tubingen, Tubingen, Germany. AD - Center for Soft Tissue Sarcomas, GIST and Bone Tumors (ZWS) of the University Hospital Tubingen and the Comprehensive Cancer Center (CCC) Tubingen-Stuttgart, Tubingen, Germany. AD - DFG Cluster of Excellence 2180 'Image-Guided and Functional Instructed Tumor Therapy' (iFIT), University of Tubingen, Tubingen, Germany. FAU - Biskup, Saskia AU - Biskup S AD - Center for Human Genetics Tubingen, Tubingen, Germany. AD - CeGaT GmbH, Tubingen, Germany. FAU - Deinzer, Christoph K W AU - Deinzer CKW AD - Department of Internal Medicine VIII - Medical Oncology and Pneumology, University Hospital Tubingen, Tubingen, Germany. christoph.deinzer@med.uni-tuebingen.de. AD - Center for Soft Tissue Sarcomas, GIST and Bone Tumors (ZWS) of the University Hospital Tubingen and the Comprehensive Cancer Center (CCC) Tubingen-Stuttgart, Tubingen, Germany. christoph.deinzer@med.uni-tuebingen.de. AD - DFG Cluster of Excellence 2180 'Image-Guided and Functional Instructed Tumor Therapy' (iFIT), University of Tubingen, Tubingen, Germany. christoph.deinzer@med.uni-tuebingen.de. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - BMC Cancer JT - BMC cancer JID - 100967800 RN - 0 (Circulating Tumor DNA) RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - *Circulating Tumor DNA/blood/genetics MH - *Sarcoma/genetics/blood/diagnostic imaging/pathology/therapy MH - Female MH - Retrospective Studies MH - Male MH - *Biomarkers, Tumor/blood/genetics MH - Middle Aged MH - Neoplasm, Residual/genetics MH - Adult MH - Aged MH - *Bone Neoplasms/genetics/blood/diagnostic imaging PMC - PMC13715185 OTO - NOTNLM OT - Bone sarcoma OT - Circulating tumor DNA (ctDNA) OT - Disease monitoring OT - Liquid biopsy OT - Molecular residual disease OT - Real-world cohort OT - Soft tissue sarcoma COIS- Declarations. Ethics approval and consent to participate: This retrospective study was conducted in accordance with the Declaration of Helsinki and was approved by the Ethics Committee of the Faculty of Medicine of the University of Tubingen and the University Hospital Tubingen, Germany (approval number 194/2025BO2). All patients had provided the University Hospital Tubingen Biomaterial Broad Consent for the use of biomaterial and associated clinical data for research purposes. The requirement for additional study-specific written informed consent for this retrospective analysis of pseudonymized data was waived by the ethics committee. Consent for publication: Not applicable. Competing interests: CKWD is a consulting or advisory board member for Boehringer Ingelheim. He has received lecture honoraria from PharmaMar. In addition, he has acceptedimbursement for travel, accommodation or congress registration expenses from Boehringer Ingelheim, Deciphera, Alexion, Lilly, Sanofi and Pierre Fabre (alloutside the submitted work). KB has accepted imbursement for travel, accommodation or congress registration expenses from Boehringer Ingelheim, Pierre Fabre and Lilly (all outside the submitted work). The other authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/27 CRDT- 2026/10/28 00:36 PHST- 2026/06/27 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:36 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 10.1186/s12885-026-17180-y [pii] AID - 17180 [pii] AID - 10.1186/s12885-026-17180-y [doi] PST - epublish SO - BMC Cancer. 2026 Oct 27;26(1):1311. doi: 10.1186/s12885-026-17180-y. PMID- 42947035 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1471-2377 (Electronic) IS - 1471-2377 (Linking) VI - 26 IP - 1 DP - 2026 Sep 3 TI - The application of brain-computer interface combined with neuromodulation for stroke upper-limb rehabilitation: a systematic review and network meta-analysis. LID - 10.1186/s12883-026-05343-0 [doi] LID - 673 AB - BACKGROUND: In conventional rehabilitation, upper-limb recovery in patients with stroke is slow and limited. Motor Imagery-Based Brain-Computer Interface (MI-BCI) may promote cortical plasticity by translating brain signals into commands to control external devices; however, the effects of different intervention approaches remain unclear. METHODS: This study followed the PRISMA 2020 statement and searched PubMed, EBSCO, Web of Science, Scopus, the Cochrane Library, and the China National Knowledge Infrastructure (CNKI) for randomised controlled trials (RCTs) reporting outcomes related to upper-limb function or activities of daily living. Risk of Bias 2 (RoB 2) and Confidence in Network Meta-Analysis (CINeMA) were used to assess risk of bias and confidence in the evidence. Network meta-analysis was conducted using the network and mvmeta modules in Stata 18.0 to assess heterogeneity, network consistency, robustness of the results, and publication bias. RESULTS: A total of 49 studies involving 2,209 participants and 13 intervention strategies were included. For the Fugl-Meyer Assessment of the Upper Extremity (FMA-UE), interventions showing relatively favourable effects included MI-BCI combined with transcranial ultrasound stimulation and functional electrical stimulation (FES) (MD = 17.65, 95% CI: 6.88 to 28.43), MI-BCI combined with repetitive transcranial magnetic stimulation (rTMS) and rehabilitation robotics (ROB) (MD = 14.22, 95% CI: 2.98 to 25.47), and MI-BCI combined with transcranial direct current stimulation (tDCS) and ROB (MD = 13.59, 95% CI: 6.90 to 20.28). For the Action Research Arm Test (ARAT), favourable interventions included MI-BCI combined with ROB and tDCS (MD = 17.26, 95% CI: 5.96 to 28.55) and MI-BCI combined with FES (MD = 14.94, 95% CI: 9.10 to 20.78). For the Wolf Motor Function Test (WMFT), MI-BCI combined with ROB showed the largest effect (MD = 10.72, 95% CI: 9.44 to 17.18). For the Modified Barthel Index (MBI), favorable interventions included MI-BCI combined with tDCS and ROB (MD = 20.40, 95% CI: 11.21 to 29.60), MI-BCI combined with rTMS and ROB (MD = 19.46, 95% CI: 9.33 to 29.59), and MI-BCI combined with intermittent theta-burst stimulation and ROB (MD = 18.24, 95% CI: 5.93 to 30.55). Overall, the network evidence showed no obvious inconsistency; between-study heterogeneity was within an acceptable range, and no clear publication bias was detected. CONCLUSION: Some non-invasive MI-BCI combined interventions like neuromodulation, ROB, or FES might improve upper-limb function and daily activities in stroke patients. However, rankings don't imply clinical importance. Due to small samples, limited evidence, and differences, these findings need confirmation in larger trials. CI - (c) 2026. The Author(s). FAU - Zhu, Hede AU - Zhu H AD - School of Health and Physical Education, Shangluo University, Shangluo City, Shaanxi, China. FAU - Kang, Cong AU - Kang C AD - School of Health and Physical Education, Shangluo University, Shangluo City, Shaanxi, China. FAU - Luo, Yuanyuan AU - Luo Y AD - Dazhou Vocational College of Chinese Medicine, Dazhou, Sichuan, China. FAU - Huang, Xianzhong AU - Huang X AD - School of Health and Physical Education, Shangluo University, Shangluo City, Shaanxi, China. 236002@slxy.edu.cn. FAU - Koh, Denise AU - Koh D AUID- ORCID: 0000-0002-5086-219X AD - Centre for Community Education and Well-Being, Faculty of Education, Universiti Kebangsaan Malaysia, UKM, Bangi, Selangor, 43600, Malaysia. denise.koh@ukm.edu.my. FAU - Mazalan, Nur Shakila AU - Mazalan NS AD - Centre for Community Education and Well-Being, Faculty of Education, Universiti Kebangsaan Malaysia, UKM, Bangi, Selangor, 43600, Malaysia. FAU - Cao, Yingying AU - Cao Y AD - School of Physical Education, Xi'an University, Xi'an, Shaanxi, 710065, China. FAU - Luo, Zhongjian AU - Luo Z AD - Dazhou Vocational College of Chinese Medicine, Dazhou, Sichuan, China. LA - eng PT - Journal Article PT - Network Meta-Analysis PT - Systematic Review DEP - 20260903 PL - England TA - BMC Neurol JT - BMC neurology JID - 100968555 SB - IM MH - Humans MH - *Stroke Rehabilitation/methods MH - *Brain-Computer Interfaces MH - *Upper Extremity/physiopathology MH - *Stroke/therapy/physiopathology MH - Transcranial Magnetic Stimulation/methods MH - Transcranial Direct Current Stimulation/methods MH - Electric Stimulation Therapy/methods MH - Recovery of Function PMC - PMC13711703 OTO - NOTNLM OT - Brain-computer interface OT - Functional electrical stimulation OT - Motor imagery OT - Network meta-analysis OT - Robotic rehabilitation OT - Stroke COIS- Declarations. Ethics approval and consent to participate: Ethical approval and informed consent for the original trials were obtained from the respective institutional review boards, as reported in the included publications. Since this study is a systematic review and meta-analysis of published data, no new human participants were recruited, and no additional ethical approval or consent was required. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/09/03 CRDT- 2026/10/28 00:36 PHST- 2026/04/15 00:00 [received] PHST- 2026/08/27 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:36 [entrez] PHST- 2026/09/03 00:00 [pmc-release] AID - 10.1186/s12883-026-05343-0 [pii] AID - 5343 [pii] AID - 10.1186/s12883-026-05343-0 [doi] PST - epublish SO - BMC Neurol. 2026 Sep 3;26(1):673. doi: 10.1186/s12883-026-05343-0. PMID- 42947028 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1471-244X (Electronic) IS - 1471-244X (Linking) VI - 26 IP - 1 DP - 2026 Jul 30 TI - Plasma exosomal microRNA profiles associated with agitation symptoms in schizophrenia. LID - 10.1186/s12888-026-08459-2 [doi] LID - 834 AB - BACKGROUND: Inflammatory dysregulation and synaptic dysfunction have been implicated in the pathophysiology of agitation in schizophrenia (SZ), but the underlying molecular mechanisms remain unclear. Plasma exosomal microRNAs (miRNAs) are stable circulating molecules that may reflect disease-related molecular alterations associated with schizophrenia. This study aimed to investigate plasma exosomal miRNA expression profiles and peripheral inflammatory factors in schizophrenia patients with agitation, and to identify potential biomarkers for agitation symptoms. METHODS: In this study, plasma exosomal miRNA expression profiles and peripheral inflammatory factors were compared between schizophrenia patients with agitation assessed within 24 h of hospital admission using PANSS-EC (SZA group, n = 35) and those without agitation (SZN group, n = 29). Differentially expressed plasma exosomal miRNAs were identified and subjected to GO and KEGG enrichment analyses. Peripheral inflammatory factors, including cytokine levels, neutrophil counts, and neutrophil-to-albumin ratio (NAR), were also measured. Partial correlation analyses (adjusting for age, sex, BMI, chlorpromazine equivalent, and diazepam equivalent) with Bonferroni correction were performed. A binary logistic regression (LR) model with forward stepwise selection (fixed covariates: age, sex, BMI, chlorpromazine equivalent, and diazepam equivalent) was constructed to identify discriminating factors, followed by receiver operating characteristic (ROC) curve analysis and five‑fold cross‑validation. RESULTS: A total of 18 differentially expressed plasma exosomal miRNAs (15 upregulated, 3 downregulated) were identified in the SZA group. Target genes of upregulated miRNAs were enriched in MAPK and Ras signaling pathways, as well as cholinergic synapse and efferocytosis. Conversely, target genes of the downregulated miRNAs were specifically enriched in glutamatergic synapse. NAR showed a trend toward elevation in the SZA group (uncorrected p = 0.004, Bonferroni‑adjusted p = 0.088), while no significant differences in cytokine levels were observed. Partial correlation analysis revealed that in the SZA group, IL‑12p70 was positively correlated with miR‑3620‑5p (r = 0.893, adjusted p < 0.001) and miR‑17‑3p (r = 0.768, adjusted p = 0.028). Multivariable logistic regression (including fixed covariates: age, sex, BMI, chlorpromazine equivalent, diazepam equivalent) retained NAR (OR = 3.778, p = 0.006) and miR‑543 (OR = 0.162, p = 0.026). The combined model (NAR + miR‑543 + fixed covariates) achieved an AUC of 0.812, outperforming single‑marker models (NAR AUC = 0.752, miR‑543 AUC = 0.715). Five‑fold cross‑validation yielded a mean AUC of 0.795 (95% CI 0.675-0.916), with sensitivity 80.1 +/- 16.5%, specificity 79.5 +/- 26.1%, and accuracy 77.8 +/- 8.9%. CONCLUSION: The findings suggest that schizophrenia patients with agitation exhibit distinct plasma exosomal miRNA expression profiles and peripheral inflammatory alterations compared to those without agitation. These findings also imply potential involvement of inflammation-associated signaling and synaptic pathways in the biological mechanisms underlying agitation in schizophrenia. Furthermore, the combination of NAR and miR-543 may serve as a potential biomarker for agitation assessment in schizophrenia, offering new insights into the biological mechanisms underlying this clinically challenging symptom. CI - (c) 2026. The Author(s). FAU - Ling, Yucheng AU - Ling Y AD - Department of Psychiatry, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, China. FAU - Wang, Yifan AU - Wang Y AD - Department of Psychiatry, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, China. FAU - Zhu, Ruiqiu AU - Zhu R AD - Department of Psychiatry, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, China. FAU - Tang, Yilin AU - Tang Y AD - Department of Psychiatry, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, China. FAU - Tao, Ran AU - Tao R AD - Department of Psychiatry, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, China. FAU - Li, Yantong AU - Li Y AD - Department of Psychiatry, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, China. FAU - Sui, Yuxiu AU - Sui Y AD - Department of Psychiatry, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, China. suiyuxiu@aliyun.com. LA - eng GR - 201715048;ZKX17030/Nanjing Medical University/ PT - Journal Article DEP - 20260730 PL - England TA - BMC Psychiatry JT - BMC psychiatry JID - 100968559 RN - 0 (MicroRNAs) RN - 0 (Biomarkers) RN - 0 (Cytokines) SB - IM MH - Humans MH - *Schizophrenia/blood/complications/genetics MH - *Exosomes/genetics/metabolism MH - *MicroRNAs/blood MH - Female MH - Male MH - Biomarkers/blood MH - Adult MH - *Psychomotor Agitation/blood/complications/genetics MH - Middle Aged MH - Cytokines/blood PMC - PMC13715222 OTO - NOTNLM OT - Agitation OT - Exosomes OT - MicroRNAs OT - Schizophrenia COIS- Declarations. Ethics approval and consent to participate: This study was approved by the Ethics Committee of The Affiliated Brain Hospital of Nanjing Medical University, Jiangsu, China (Ethic approval numbers:2024-KY064-02) and was conducted in accordance with the ethical standards set out in the 1964 Declaration of Helsinki and its subsequent amendments. All patients signed informed consent forms, with their anonymity was fully protected. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/30 CRDT- 2026/10/28 00:35 PHST- 2026/03/25 00:00 [received] PHST- 2026/07/24 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:35 [entrez] PHST- 2026/07/30 00:00 [pmc-release] AID - 10.1186/s12888-026-08459-2 [pii] AID - 8459 [pii] AID - 10.1186/s12888-026-08459-2 [doi] PST - epublish SO - BMC Psychiatry. 2026 Jul 30;26(1):834. doi: 10.1186/s12888-026-08459-2. PMID- 42947026 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1471-2407 (Electronic) IS - 1471-2407 (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Characterization of etoposide-resistant choriocarcinoma cells and contribution of MAPK7 to etoposide resistance. LID - 10.1186/s12885-026-16640-9 [doi] LID - 1310 AB - BACKGROUND: Gestational choriocarcinoma is an aggressive gynecologic malignancy that can occur with various pregnancy events. Although typically highly sensitive to combination chemotherapy, this malignancy, in a subset of patients, develops resistance to etoposide-containing regimens, leading to therapeutic failure. We aimed to elucidate the molecular mechanisms that confer resistance to etoposide, a central component of choriocarcinoma chemotherapy. METHODS: Etoposide-resistant JAR and JEG-3 choriocarcinoma cell lines were established by continuous stepwise exposure of parental cells to increasing concentrations of etoposide. Phenotypic comparisons with the parental cells were then performed. RNA sequencing and upstream regulator analyses were conducted to identify key regulatory molecules and signaling pathways associated with etoposide resistance. Candidate molecules were further evaluated by siRNA-mediated knockdown to assess their effects on etoposide sensitivity. RESULTS: Compared with their parental cells, etoposide-resistant choriocarcinoma cells demonstrated significantly reduced proliferation (p < 0.001), migration (p < 0.05), and invasion (p < 0.01). RNA sequencing and pathway analyses revealed suppression of pathways related to cell cycle progression and DNA replication. In addition, upstream regulator analysis focusing on kinases suggested the involvement of MAPK-related molecules, including MAPK9, MAP2K5, and MAPK7. siRNA-mediated suppression of MAPK7 in resistant cells restored sensitivity to etoposide, suggesting a role for MAPK7 in the maintenance of etoposide resistance. CONCLUSION: Our findings indicate that the MAPK signaling pathway, particularly MAPK7, contributes to etoposide resistance in choriocarcinoma, based on RNA sequencing and functional analyses of resistant cell models. CI - (c) 2026. The Author(s). FAU - Yasui, Yuko AU - Yasui Y AD - Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Tsuruma-Cho 65, Showa-Ku, Nagoya, 466-8550, Japan. AD - Department of Obstetrics and Gynecology, Aichi Medical University School of Medicine, Nagakute, Japan. FAU - Yoshida, Kosuke AU - Yoshida K AUID- ORCID: 0000-0003-1484-4872 AD - Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Tsuruma-Cho 65, Showa-Ku, Nagoya, 466-8550, Japan. yoshida.kousuke@med.nagoya-u.ac.jp. AD - Institute for Advanced Research, Nagoya University, Nagoya, Japan. yoshida.kousuke@med.nagoya-u.ac.jp. FAU - Nishiko, Yuki AU - Nishiko Y AD - Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Tsuruma-Cho 65, Showa-Ku, Nagoya, 466-8550, Japan. AD - Department of Obstetrics and Gynecology, Aichi Medical University School of Medicine, Nagakute, Japan. FAU - Suzuki, Hironori AU - Suzuki H AD - Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Tsuruma-Cho 65, Showa-Ku, Nagoya, 466-8550, Japan. AD - Laboratory of Integrative Oncology, National Cancer Center Research Institute, Tokyo, Japan. FAU - Yamamoto, Yusuke AU - Yamamoto Y AD - Laboratory of Integrative Oncology, National Cancer Center Research Institute, Tokyo, Japan. FAU - Mogi, Kazumasa AU - Mogi K AD - Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Tsuruma-Cho 65, Showa-Ku, Nagoya, 466-8550, Japan. AD - Department of Medical Genomics Center, Nagoya University Hospital, Nagoya, Japan. FAU - Yoshihara, Masato AU - Yoshihara M AD - Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Tsuruma-Cho 65, Showa-Ku, Nagoya, 466-8550, Japan. FAU - Nagao, Yukari AU - Nagao Y AD - Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Tsuruma-Cho 65, Showa-Ku, Nagoya, 466-8550, Japan. FAU - Tamauchi, Satoshi AU - Tamauchi S AD - Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Tsuruma-Cho 65, Showa-Ku, Nagoya, 466-8550, Japan. FAU - Yokoi, Akira AU - Yokoi A AD - Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Tsuruma-Cho 65, Showa-Ku, Nagoya, 466-8550, Japan. AD - Institute for Advanced Research, Nagoya University, Nagoya, Japan. FAU - Yoshikawa, Nobuhisa AU - Yoshikawa N AD - Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Tsuruma-Cho 65, Showa-Ku, Nagoya, 466-8550, Japan. FAU - Nishino, Kimihiro AU - Nishino K AD - Department of Healthcare Administration, Nagoya University Graduate School of Medicine, Nagoya, Japan. FAU - Yamamoto, Eiko AU - Yamamoto E AD - Department of Healthcare Administration, Nagoya University Graduate School of Medicine, Nagoya, Japan. FAU - Niimi, Kaoru AU - Niimi K AUID- ORCID: 0000-0003-4546-9606 AD - Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Tsuruma-Cho 65, Showa-Ku, Nagoya, 466-8550, Japan. kaorun@med.nagoya-u.ac.jp. FAU - Kajiyama, Hiroaki AU - Kajiyama H AD - Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, Tsuruma-Cho 65, Showa-Ku, Nagoya, 466-8550, Japan. LA - eng GR - 25K12699/Japan Society for the Promotion of Science/ GR - 25K02414/Japan Society for the Promotion of Science/ GR - 23K08864/Japan Society for the Promotion of Science/ PT - Journal Article DEP - 20260731 PL - England TA - BMC Cancer JT - BMC cancer JID - 100967800 RN - 6PLQ3CP4P3 (Etoposide) RN - 0 (Antineoplastic Agents, Phytogenic) SB - IM MH - Humans MH - *Etoposide/pharmacology/therapeutic use MH - *Drug Resistance, Neoplasm/genetics MH - *Choriocarcinoma/drug therapy/genetics/pathology/metabolism MH - Female MH - Cell Line, Tumor MH - Cell Proliferation/drug effects MH - *Antineoplastic Agents, Phytogenic/pharmacology MH - Gene Expression Regulation, Neoplastic/drug effects MH - Pregnancy MH - *Uterine Neoplasms/drug therapy/genetics/pathology MH - Cell Movement/drug effects PMC - PMC13711928 OTO - NOTNLM OT - Choriocarcinoma OT - Etoposide-resistant OT - MAPK pathway OT - MAPK7 OT - RNA-seq COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors have no conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:35 PHST- 2026/04/27 00:00 [received] PHST- 2026/07/24 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:35 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1186/s12885-026-16640-9 [pii] AID - 16640 [pii] AID - 10.1186/s12885-026-16640-9 [doi] PST - epublish SO - BMC Cancer. 2026 Jul 31;26(1):1310. doi: 10.1186/s12885-026-16640-9. PMID- 42947025 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261028 LR - 20261029 IS - 1865-1372 (Print) IS - 1865-1380 (Electronic) IS - 1865-1372 (Linking) VI - 19 IP - 1 DP - 2026 Oct 27 TI - Physiological monitoring and physiology-informed strategies in cardiac arrest: current evidence and clinical translation. LID - 10.1186/s12245-026-01419-3 [doi] LID - 276 AB - BACKGROUND: Despite advances in cardiac arrest management, survival with favorable neurological outcomes remains limited. Physiological monitoring may complement standardized resuscitation by providing real-time information about circulatory effectiveness, reversible causes, and treatment responses. However, whether physiology-guided interventions improve patient-centered outcomes remains uncertain. This narrative review examines the current evidence and clinical applicability of physiological monitoring during resuscitation, physiology-informed rescue strategies, and individualized post-cardiac arrest care. MAIN BODY: During cardiopulmonary resuscitation, invasive arterial pressure monitoring, waveform capnography, and echocardiography provide complementary information about the circulation generated by chest compressions and potential mechanisms of resuscitation failure. Although observational and experimental studies support their physiological relevance, no adult diastolic pressure target or pressure-guided vasopressor strategy has been validated to improve survival or neurological recovery. Recent randomized trials of transesophageal echocardiography have demonstrated physiological or procedural effects without establishing clinical benefit. Among rescue strategies, extracorporeal cardiopulmonary resuscitation has yielded inconsistent results across randomized trials, with potential benefits dependent on patient selection, intervention timing, and system capabilities. Resuscitative endovascular balloon occlusion of the aorta has not improved clinical outcomes in randomized evaluation, while head-up resuscitation remains investigational. Following the return of spontaneous circulation, randomized trials have challenged the benefits of uniformly higher blood pressure and oxygenation targets, mild hypercapnia, and deeper or prolonged temperature control. Individualized approaches incorporating cerebral autoregulation, venous congestion, and cardiopulmonary physiology are physiologically appealing but lack prospective validation. CONCLUSION: Physiological assessment may complement established resuscitation algorithms by refining clinical decisions during and after cardiac arrest. However, improvements in physiological measurements should not be equated with improved patient outcomes. Future trials should determine whether predefined, physiology-guided treatment strategies improve survival and neurological recovery. CI - (c) 2026. The Author(s). FAU - Melo, Rafael Hortencio AU - Melo RH AUID- ORCID: 0000-0001-6685-6002 AD - Faculdade Israelita de Ciencias de Saude Albert Einstein, Hospital Israelita Albert Einstein, Av. Santa Catarina, 2785 - Vila Santa Catarina, Sao Paulo, SP, Brazil. rafaelmeloufc@yahoo.com.br. AD - Hcor Research Institute, Hospital do Coracao, Sao Paulo, SP, Brazil. rafaelmeloufc@yahoo.com.br. AD - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil. rafaelmeloufc@yahoo.com.br. FAU - Joelsons, Daniel AU - Joelsons D AD - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil. FAU - Guimaraes, Helio Penna AU - Guimaraes HP AD - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil. FAU - Passos, Rogerio da Hora AU - Passos RDH AD - Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - England TA - Int J Emerg Med JT - International journal of emergency medicine JID - 101469435 PMC - PMC13712052 OTO - NOTNLM OT - Cardiac arrest OT - Cardiopulmonary resuscitation OT - Extracorporeal cardiopulmonary resuscitation OT - Hemodynamic monitoring OT - Neurologic outcome COIS- Declarations. Ethics approval and consent to participate: This article is a narrative review of previously published literature and did not involve human participants, human data, human tissues, or animal experimentation performed by the authors. Therefore, ethics committee approval and informed consent were not required. Consent for publication: Not applicable. Declaration of generative AI and AI-assisted technologies in the manuscript preparation process: During the preparation of this manuscript, the authors used ChatGPT (OpenAI) and other generative artificial intelligence tools to assist with language editing and manuscript organization. BioRender was used to create and assemble elements of the figures. All AI-assisted content was critically reviewed and revised by the authors, who take full responsibility for the final content of the article. Competing interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/27 CRDT- 2026/10/28 00:35 PHST- 2026/07/24 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:35 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 10.1186/s12245-026-01419-3 [pii] AID - 1419 [pii] AID - 10.1186/s12245-026-01419-3 [doi] PST - epublish SO - Int J Emerg Med. 2026 Oct 27;19(1):276. doi: 10.1186/s12245-026-01419-3. PMID- 42947023 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1471-230X (Electronic) IS - 1471-230X (Linking) VI - 26 IP - 1 DP - 2026 Jul 31 TI - Clinical outcomes and repeat ERCP rates following plastic and metal stent placement in distal biliary strictures: a retrospective cohort study. LID - 10.1186/s12876-026-05068-x [doi] LID - 706 AB - BACKGROUND: Distal biliary strictures may lead to biliary obstruction and serious clinical complications. Endoscopic retrograde cholangiopancreatography (ERCP) with stent placement is commonly used for biliary drainage; however, the comparative effectiveness of different stent types remains uncertain. This study aimed to compare clinical outcomes and repeat ERCP requirements among patients receiving 7 Fr plastic stents (PS), 10 Fr PS, or fully covered self-expandable metal stents (FCSEMS) during index ERCP for distal biliary strictures. METHODS: This retrospective observational cohort study included 100 adult patients who underwent ERCP for distal biliary strictures between 2015 and 2025. Patients were categorized according to the stent type placed during the initial ERCP: 7 Fr PS (n=45), 10 Fr PS (n=35), and FCSEMS (n=20). Biochemical parameters were evaluated before ERCP, on post-procedural day 3, and at week 3. Post-ERCP pancreatitis (PEP), bleeding, cholangitis, and repeat ERCP requirement within 3 months were assessed. Appropriate parametric and non-parametric statistical analyses were performed. RESULTS: In the 7 Fr group, the reduction in total bilirubin levels on day 3 did not reach statistical significance (p=0.07), although significant improvements were observed in other biochemical parameters. By week 3, all groups demonstrated significant biochemical improvement (p/= 10% mucosal ulceration, mixed inflammation expanding the lamina propria and extending into surrounding intestinal layers, and dissolution of the stratum granulosum. In chronic ASE lesions, we further characterized granulation tissue formation. Cold water holding of spawning adults appears to slow disease progression and reduce PSM without preventing ASE development. These findings demonstrate that ASE is a geographically restricted disease of the Columbia Basin that may have associations with diverse environmental exposures including water temperature and pathogens. CI - (c) 2026 John Wiley & Sons Ltd. FAU - Polley, Tamsen M AU - Polley TM AUID- ORCID: 0000-0002-8978-1020 AD - Department of Biomedical Sciences and Oregon Veterinary Diagnostic Laboratory, Oregon State University, Corvallis, Oregon, USA. AD - Wyoming State Veterinary Laboratory and Department of Veterinary Sciences, University of Wyoming, Laramie, Wyoming, USA. FAU - Pose, Roberto Bermudez AU - Pose RB AUID- ORCID: 0000-0003-4969-4122 AD - Research Group GapaVET, Institute for Research in Global Health and Sustainable Development, iTERRA, Department of Anatomy, Animal Production and Veterinary Clinical Sciences, Campus Terra, Universidade de Santiago de Compostela, Lugo, Spain. FAU - Lohr, Christiane V AU - Lohr CV AUID- ORCID: 0000-0003-3787-5583 AD - Department of Biomedical Sciences and Oregon Veterinary Diagnostic Laboratory, Oregon State University, Corvallis, Oregon, USA. FAU - Hickey, Nora AU - Hickey N AUID- ORCID: 0009-0006-4320-8882 AD - Washington Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA. FAU - Strickland, Stacy AU - Strickland S AUID- ORCID: 0009-0006-3494-4992 AD - Oregon Department of Fish and Wildlife, Madras Fish Health Laboratory, Madras, Oregon, USA. FAU - Schuster, Corbin L AU - Schuster CL AUID- ORCID: 0000-0002-9147-9146 AD - Department of Microbiology, Oregon State University, Corvallis, Oregon, USA. FAU - Kent, Michael L AU - Kent ML AUID- ORCID: 0000-0001-7968-5142 AD - Department of Biomedical Sciences and Oregon Veterinary Diagnostic Laboratory, Oregon State University, Corvallis, Oregon, USA. AD - Department of Microbiology, Oregon State University, Corvallis, Oregon, USA. LA - eng GR - Margaret I. Lenhard Fellowship/ GR - USDA-APHIS ORISE Fellowship/ GR - GPC GI-1707/Xunta de Galicia/ PT - Journal Article DEP - 20261027 PL - England TA - J Fish Dis JT - Journal of fish diseases JID - 9881188 SB - IM OTO - NOTNLM OT - Chinook Salmon OT - adult salmon enteritis OT - microsporidia OT - prespawn mortality EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:25 PHST- 2026/10/12 00:00 [revised] PHST- 2026/07/29 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:25 [entrez] AID - e70317 [pii] AID - 10.1111/jfd.70317 [doi] PST - aheadofprint SO - J Fish Dis. 2026 Oct 27:e70317. doi: 10.1111/jfd.70317. PMID- 42946955 OWN - NLM STAT- Publisher LR - 20261028 IS - 1556-0961 (Electronic) IS - 1541-6933 (Linking) DP - 2026 Oct 27 TI - High Postoperative Perihematomal Edema Trajectory after Endoscopic Evacuation for Supratentorial Intracerebral Hemorrhage. LID - 10.1007/s12028-026-02707-9 [doi] AB - BACKGROUND AND OBJECTIVE: The clinical relevance of perihematomal edema (PHE) after endoscopic evacuation of supratentorial intracerebral hemorrhage (ICH) is uncertain. We examined a longitudinal postoperative PHE phenotype and its association with 90-day functional outcome. METHODS: This two-center retrospective cohort included 136 patients who survived beyond 72 h after endoscopic evacuation of supratentorial ICH and had complete serial computed tomography (CT) and 90-day modified Rankin Scale (mRS) assessment. CT windows were 0-24 h (T1), 48-72 h (T2), and day 5-7 (T3). Before outcome-model fitting, high postoperative PHE trajectory was defined as T3 PHE >/= 36.6 mL or continuous increase across T1-T2-T3 with a T3-T1 change >/= 7.7 mL; both thresholds were cohort-derived upper quartiles. Center-adjusted logistic regression examined associated factors and poor 90-day outcome (mRS score 3-6). RESULTS: High postoperative PHE trajectory occurred in 51 patients (37.5%). Intraventricular hemorrhage (IVH) severity (adjusted odds ratio [aOR] 2.11 per category, 95% confidence interval [CI] 1.09-4.09), residual hematoma volume (aOR 1.75 per mL, 95% CI 1.35-2.27), and fever duration within 72 h (aOR 1.20 per h, 95% CI 1.08-1.34) were associated with this trajectory. Adding trajectory to the outcome model increased apparent area under the receiver operating characteristic curve (AUC) from 0.846 to 0.905; trajectory was associated with poor outcome (aOR 25.21, 95% CI 6.13-103.60). Among 131 paired observations, mRS score 0-2 occurred in 71/131 (54.2%) at 90 days and 76/131 (58.0%) at 12 months (two-sided exact McNemar P = 0.125). CONCLUSIONS: In this selected cohort of early postoperative survivors, IVH severity, residual hematoma volume, and fever duration were associated with high postoperative PHE trajectory, which was associated with poor 90-day functional outcome. This cohort-derived imaging phenotype warrants external validation as a candidate prognostic marker after endoscopic evacuation. CI - (c) 2026. Springer Science+Business Media, LLC, part of Springer Nature and Neurocritical Care Society. FAU - Liu, Shuang AU - Liu S AD - Department of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. FAU - Li, Chen AU - Li C AD - Department of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. FAU - Su, Shengyang AU - Su S AD - Department of Surgery, People's Hospital of Jinping Miao, Yao and Dai Autonomous County, Kunming, 661599, Yunnan Province, China. FAU - Long, Jinyong AU - Long J AD - Department of Surgery, People's Hospital of Jinping Miao, Yao and Dai Autonomous County, Kunming, 661599, Yunnan Province, China. FAU - Zhao, Puyuan AU - Zhao P AD - Department of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. FAU - Xie, Tao AU - Xie T AD - Department of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. FAU - Xie, Qiang AU - Xie Q AD - Department of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. FAU - Zhang, Xiaobiao AU - Zhang X AUID- ORCID: 0000-0003-3970-1106 AD - Department of Neurosurgery, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. xiaobiao_zhang@163.com. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Neurocrit Care JT - Neurocritical care JID - 101156086 SB - IM OTO - NOTNLM OT - Endoscopic evacuation OT - Functional outcome OT - Intracerebral Hemorrhage OT - Perihematomal edema OT - Postoperative fever OT - Residual hematoma COIS- Conflicts of Interests: The authors report no relevant financial or nonfinancial conflicts of interest. Ethical Approval/Informed Consent: This study was approved by the Ethics Committee of the People's Hospital of Jinping Miao, Yao and Dai Autonomous County (approval no. 2025-LL-0005) and the Ethics Committee of Zhongshan Hospital, Fudan University (approval no. 2025-B053). The study was conducted in accordance with the Declaration of Helsinki and its later amendments. For this type of retrospective study, formal consent was not required. Consent to publish is not applicable because no identifying patient information is presented. Artificial Intelligence: The artificial intelligence (AI)-assisted volumetric module within uAI Research Portal (uRP; United Imaging Intelligence Co., Ltd.) was used only to calculate volumes from final investigator-approved masks. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:25 PHST- 2026/06/09 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:25 [entrez] AID - 10.1007/s12028-026-02707-9 [pii] AID - 10.1007/s12028-026-02707-9 [doi] PST - aheadofprint SO - Neurocrit Care. 2026 Oct 27. doi: 10.1007/s12028-026-02707-9. PMID- 42946954 OWN - NLM STAT- Publisher LR - 20261028 IS - 1614-7499 (Electronic) IS - 0944-1344 (Linking) DP - 2026 Oct 28 TI - Nickel-modified cobalt phosphate: physicochemical properties and catalytic performance in the total oxidation of ethanol. LID - 10.1007/s11356-026-38336-0 [doi] AB - Nickel-incorporated cobalt phosphate (Ni-CoP) catalysts were synthesized via co-precipitation and impregnation to explore the correlation between their structural properties and catalytic performance in ethanol oxidation. Advanced characterization (XRD, BET, DRIFTS, FESEM/EDX, XPS, H(2)-TPR, NH(3)-TPD, CO(2)-TPD) confirmed that incorporation of 10 wt% Ni decreased crystallite size and acidity, increased basicity, and facilitated redox activity through the formation of a NiCoP(4)O(12) mixed phase. These physicochemical changes enabled complete ethanol conversion at 310 degrees C with a CO(2) selectivity of 96%, outperforming undoped CoP, which achieved complete conversion at 328 degrees C. The Ni-CoP catalyst also maintained high activity and selectivity across multiple reaction cycles, exhibiting a modest shift in light-off temperature. These findings underscore the critical role of acid-base surface modification and synergistic metal-phosphate interactions in the development of cost-effective and stable catalysts for VOC abatement. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Mokhliss, Souad AU - Mokhliss S AUID- ORCID: 0000-0003-1854-1836 AD - Laboratory of Coordination and Analytical Chemistry, Faculty of Sciences, Chouaib Doukkali University, Ben Maachou Road, B.P:20, 24000, El Jadida, Morocco. mokhliss.s@ucd.ac.ma. AD - Environmental and Chemical Engineering (ECE), Faculty of Technology, University of Oulu, 90014, Oulu, Finland. mokhliss.s@ucd.ac.ma. FAU - Laitinen, Tiina AU - Laitinen T AUID- ORCID: 0000-0002-8441-4862 AD - Environmental and Chemical Engineering (ECE), Faculty of Technology, University of Oulu, 90014, Oulu, Finland. FAU - Majoudi, Noura AU - Majoudi N AD - Process Metallurgy Research Unit, Faculty of Technology, University of Oulu, 90014, Oulu, Finland. FAU - Ojala, Satu AU - Ojala S AUID- ORCID: 0000-0001-9520-9293 AD - Environmental and Chemical Engineering (ECE), Faculty of Technology, University of Oulu, 90014, Oulu, Finland. FAU - Brahmi, Rachid AU - Brahmi R AUID- ORCID: 0000-0002-9437-1579 AD - Laboratory of Coordination and Analytical Chemistry, Faculty of Sciences, Chouaib Doukkali University, Ben Maachou Road, B.P:20, 24000, El Jadida, Morocco. FAU - Agunaou, Mahfoud AU - Agunaou M AUID- ORCID: 0000-0001-6513-6935 AD - Laboratory of Coordination and Analytical Chemistry, Faculty of Sciences, Chouaib Doukkali University, Ben Maachou Road, B.P:20, 24000, El Jadida, Morocco. LA - eng GR - 2019-1-FI01-KA107-060354/Erasmus+ Global Program/ PT - Journal Article DEP - 20261028 PL - Germany TA - Environ Sci Pollut Res Int JT - Environmental science and pollution research international JID - 9441769 SB - IM OTO - NOTNLM OT - Acid-base surface OT - Cobalt phosphate OT - Ethanol oxidation OT - Nickel OT - Redox activity OT - VOC abatement COIS- Declarations. Consent to participate: Not applicable. Consent for publication: Not applicable Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:25 PHST- 2026/07/21 00:00 [received] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:25 [entrez] AID - 10.1007/s11356-026-38336-0 [pii] AID - 10.1007/s11356-026-38336-0 [doi] PST - aheadofprint SO - Environ Sci Pollut Res Int. 2026 Oct 28. doi: 10.1007/s11356-026-38336-0. PMID- 42946949 OWN - NLM STAT- Publisher LR - 20261028 IS - 2199-1154 (Print) IS - 2198-9788 (Linking) DP - 2026 Oct 28 TI - Effects of Interventions for Delabeling Penicillin Allergy in Hospitalized Patients: A Systematic Review. LID - 10.1007/s40801-026-00594-2 [doi] AB - BACKGROUND AND OBJECTIVE: Penicillin allergy labels are common among hospitalized patients, yet most represent inaccurate or nonimmune-mediated reactions and may contribute to suboptimal antibiotic prescribing. This systematic review aimed to evaluate the effectiveness and safety of hospital-based penicillin allergy delabeling interventions and their impact on antibiotic prescribing. METHODS: PubMed/MEDLINE, CENTRAL, Scopus, and Web of Science were systematically searched up to January 2026. Additional records were identified through ClinicalTrials.gov and citation tracking. Eligible studies included hospitalized patients with documented penicillin or beta-lactam allergy labels who underwent structured delabeling interventions with a comparator group. Given substantial heterogeneity in study design, interventions, and outcomes, findings were synthesized narratively. RESULTS: Nine studies were included (three randomized controlled trials and six nonrandomized studies). Delabeling success rates ranged from approximately 4% to over 95%, depending on intervention approach, risk of bias, and local workflows. The greatest success was observed in interventions using direct oral challenge for patients at low risk of true penicillin allergy. Pharmacist-led and antimicrobial stewardship-based interventions also effectively identified inaccurate allergy labels and supported safe delabeling. Adverse events were uncommon and predominantly mild. Delabeling was associated with improved prescribing practices, including greater use of narrow-spectrum beta-lactams. CONCLUSIONS: Hospital-based penicillin allergy delabeling interventions appear safe and locally effective when implemented within structured clinical pathways. However, the current evidence base is structurally fragmented, and extreme clinical and methodological heterogeneity limits the formulation of universal hospital protocols. Standardized, multi-center randomized controlled trials are urgently needed to establish uniform risk stratification algorithms and outcome metrics. PROSPERO REGISTRATION NUMBER: CRD420251246346. CI - (c) 2026. The Author(s). FAU - Jovcic, Filip Z AU - Jovcic FZ AUID- ORCID: 0009-0005-5277-1741 AD - Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia. FAU - Milosavljevic, Milos N AU - Milosavljevic MN AUID- ORCID: 0000-0002-1786-1507 AD - Department of Pharmacology and Toxicology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000, Kragujevac, Serbia. FAU - Cmiljanic, Milena AU - Cmiljanic M AD - Medicines and Medical Devices Agency of Serbia, Belgrade, Serbia. FAU - Pejcic, Ana V AU - Pejcic AV AUID- ORCID: 0000-0003-1741-0025 AD - Department of Pharmacology and Toxicology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000, Kragujevac, Serbia. anapejcic201502@yahoo.com. FAU - Stefanovic, Srdan M AU - Stefanovic SM AUID- ORCID: 0000-0003-2206-2037 AD - Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia. LA - eng GR - 451-03-34/2026-03/200111/Ministry of Science, Technological Development and Innovations of the Republic of Serbia/ PT - Journal Article PT - Systematic Review DEP - 20261028 PL - Switzerland TA - Drugs Real World Outcomes JT - Drugs - real world outcomes JID - 101658456 COIS- Declarations. Conflicts of Interest: Filip Z. Jovcic, Milos N. Milosavljevic, Milena Cmiljanic, Ana V. Pejcic, and Srdan M. Stefanovic have no conflicts of interest that are directly relevant to the content of this article. Assistant Professor Ana V. Pejcic is an Editorial Board member of Drugs-Real World Outcomes. Ana V. Pejcic was not involved in the selection of peer reviewers for the manuscript nor any of the subsequent editorial decisions. Availability of Data and Material: The datasets generated during and/or analyzed during the current study are available on reasonable request to the corresponding author. Ethics Approval: Not applicable. Consent to Participate: Not applicable. Consent for Publication: Not applicable. Code Availability: Not applicable. Author Contributions: Conceptualization: FZJ, SMS; methodology: FZJ, AVP, SMS; software: MNM; validation: MNM, MC, SMS; formal analysis: FZJ; investigation: FZJ; resources: MNM, AVP, SMS; data curation: FZJ, MC; writing-original draft preparation: FZJ, AVP, MC; writing-review and editing: MNM, SMS; visualization: FZJ; supervision: AVP, SMS; project administration: FZJ, MNM, SMS; funding acquisition: MNM, AVP, SMS. All authors read and approved the final version. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:25 PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:25 [entrez] AID - 10.1007/s40801-026-00594-2 [pii] AID - 10.1007/s40801-026-00594-2 [doi] PST - aheadofprint SO - Drugs Real World Outcomes. 2026 Oct 28. doi: 10.1007/s40801-026-00594-2. PMID- 42946946 OWN - NLM STAT- Publisher LR - 20261028 IS - 1463-1326 (Electronic) IS - 1462-8902 (Linking) DP - 2026 Oct 27 TI - Interpreting Diabetic Retinal Outcomes After GLP-1 Receptor Agonist and Tirzepatide Initiation: Clinical Evidence and Its Limits. LID - 10.1111/dom.71490 [doi] AB - AIMS: Reports of diabetic retinopathy after potent glucose-lowering treatment create uncertainty for clinicians, particularly when studies measure different outcomes in patients with different baseline retinal disease. This review examines diabetic retinal outcomes after glucagon-like peptide-1 receptor agonist or tirzepatide initiation. MATERIALS AND METHODS: This critical narrative review uses targeted, non-systematic study selection. It distinguishes disease onset or progression from detection, treatment use and subsequent visual function. RESULTS: The SUSTAIN-6 retinopathy-complication signal remains clinically relevant; post hoc associations with early glycated haemoglobin reduction do not establish causal mediation. Contemporary cohorts report differing associations across baseline retinal strata, comparators and endpoints. Positive signals should be presented alongside their absolute event proportions and limitations, rather than dismissed because observational adjustment is imperfect. Conversely, non-significant results do not establish equivalence or class-wide safety. CONCLUSIONS: Current guidance supports establishing retinal status and coordinating diabetes and eye care during treatment intensification. The implications differ between patients with recent screening and no known retinopathy, established non-proliferative disease, and active sight-threatening disease or recent retinal treatment. No universal safe rate of glycated haemoglobin decline or retinal-protective titration regimen has been established. Future studies should align eligibility and treatment initiation, distinguish incident disease from progression, and use scheduled retinal assessments. A comparison between two specific medicines can inform treatment choice but cannot exclude risks shared by both. CI - (c) 2026 John Wiley & Sons Ltd. FAU - Wu, Ben AU - Wu B AUID- ORCID: 0000-0002-0861-861X AD - Hunan Key Laboratory of the Research and Development of Novel Pharmaceutical Preparations, School of Pharmaceutical Science, Changsha Medical University, Changsha, China. AD - Hunan Provincial Key Laboratory of TCM Agricultural Biogenomics, Changsha Medical University, Changsha, China. FAU - He, Bin-Sheng AU - He BS AD - Hunan Key Laboratory of the Research and Development of Novel Pharmaceutical Preparations, School of Pharmaceutical Science, Changsha Medical University, Changsha, China. AD - Hunan Provincial Key Laboratory of TCM Agricultural Biogenomics, Changsha Medical University, Changsha, China. LA - eng GR - 2026JJ81241/Natural Science Foundation of Hunan Province/ PT - Journal Article PT - Review DEP - 20261027 PL - England TA - Diabetes Obes Metab JT - Diabetes, obesity & metabolism JID - 100883645 SB - IM OTO - NOTNLM OT - GLP-1 receptor agonist OT - comparative safety OT - diabetic retinopathy OT - early worsening OT - retinal ascertainment OT - tirzepatide EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:25 PHST- 2026/10/13 00:00 [revised] PHST- 2026/09/08 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:25 [entrez] AID - 10.1111/dom.71490 [doi] PST - aheadofprint SO - Diabetes Obes Metab. 2026 Oct 27. doi: 10.1111/dom.71490. PMID- 42946941 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1530-9932 (Electronic) IS - 1530-9932 (Linking) VI - 27 IP - 8 DP - 2026 Oct 27 TI - NanoMiLens (Nano-Micelle Loaded Hydrogel Contact Lenses) of Tofacitinib Citrate: Amalgamation, Development, Optimization and Characterization. LID - 340 [pii] LID - 10.1208/s12249-026-03587-0 [doi] AB - Dry eye disease (DED) is a multifactorial inflammatory eye disease caused mainly by immune dysregulation through JAK/STAT, which leads to a turbulent tear film, damage to the corneal epithelia, and poor visualization. Topical therapies of the conventional type show less ocular bioavailability with quick precorneal excretion. This research was done to design and test a new NanoMiLens system loaded with Tofacitinib Citrate (TFC), which is a selective JAK inhibitor, in order to have a controlled and prolonged contact lens delivery manageable with DED. TFC Polymeric Nano-Micelles (PoT-PNMs) were prepared using a thin-film hydration method employing Poloxamer 407 as the amphiphilic polymer and Tween 80 as the stabilizing surfactant in acetone, then rehydrated with simulated tear fluid. PBD ANOVA confirmed polymer concentration and surfactant concentration as dominant factors for EE%. CCD quadratic modelling yielded R(2) = 0.9904 for EE%, 0.9789 for CDR%, and 0.9408 for particle size, with model validation errors < 1%. The optimized PoT-PNMs (200 mg Poloxamer 407, 0.30 mL Tween 80) achieved particle size 92 +/- 2.6 nm, PDI 0.246, EE% 90.60 +/- 0.44%, and CDR% 93.10 +/- 1.2% over 12 h. FTIR, DSC, and XRD confirmed drug amorphization and drug-excipient compatibility. The optimized PoT-PNMs were loaded into freeze-thaw-crosslinked PVA/pHEMA hydrogel contact lenses via soak-loading. The optimized PoT-PNMs -loaded contact lens (NMCL12) showed % transmittance 96.11 +/- 0.22%, swelling index 96.48 +/- 0.24%, drug content 90.60 +/- 0.44%, in-vitro CDR% 96.50 +/- 0.30% over 12 h, and mechanical strength 142 +/- 6 folds. This system is an effective, scalable method of topical immunomodulatory treatment of inflammatory dry eye disease. CI - (c) 2026. The Author(s), under exclusive licence to American Association of Pharmaceutical Scientists. FAU - Rana, Hardik AU - Rana H AUID- ORCID: 0000-0002-2159-7665 AD - Department of Pharmaceutics, Anand Pharmacy College, Anand, 388001, Gujarat, India. hardikrana1439@gmail.com. FAU - Bhagchandani, Yash AU - Bhagchandani Y AUID- ORCID: 0009-0004-8892-3113 AD - Department of Pharmaceutics, Anand Pharmacy College, Anand, 388001, Gujarat, India. FAU - Kher, Yuvrajsinh AU - Kher Y AUID- ORCID: 0009-0005-1468-0187 AD - Department of Pharmaceutics, Anand Pharmacy College, Anand, 388001, Gujarat, India. FAU - Thakkar, Vaishali AU - Thakkar V AUID- ORCID: 0000-0001-6332-7703 AD - Department of Pharmaceutics, Anand Pharmacy College, Anand, 388001, Gujarat, India. FAU - Gandhi, Tejal AU - Gandhi T AUID- ORCID: 0000-0002-7927-0331 AD - Department of Pharmaceutics, Anand Pharmacy College, Anand, 388001, Gujarat, India. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - AAPS PharmSciTech JT - AAPS PharmSciTech JID - 100960111 RN - 0 (Piperidines) RN - 0 (Pyrimidines) RN - 0 (Hydrogels) RN - 87LA6FU830 (tofacitinib) RN - 0 (Micelles) RN - 0 (Polymers) RN - 0 (Pyrroles) RN - 0 (Surface-Active Agents) RN - 0 (Drug Carriers) SB - IM MH - *Piperidines/chemistry/administration & dosage MH - *Pyrimidines/chemistry/administration & dosage MH - *Hydrogels/chemistry MH - Micelles MH - Particle Size MH - *Nanoparticles/chemistry MH - Polymers/chemistry MH - *Pyrroles/chemistry/administration & dosage MH - Surface-Active Agents/chemistry MH - Drug Carriers/chemistry MH - Dry Eye Syndromes/drug therapy MH - Drug Delivery Systems/methods OTO - NOTNLM OT - Dry eye disease OT - Hydrogel contact lens OT - JAK inhibitor OT - Ocular drug delivery OT - Polymeric nano-micelles OT - Quality by design OT - Tofacitinib citrate COIS- Declarations. Ethics Approval: Not applicable (in-vitro study; no animal or human subjects involved). Consent for Publication: Not applicable. Conflict of interests: The authors declare no conflict of interests. Clinical Trial Number: Not applicable. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 00:24 PHST- 2026/07/15 00:00 [received] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:24 [entrez] AID - 10.1208/s12249-026-03587-0 [pii] AID - 10.1208/s12249-026-03587-0 [doi] PST - epublish SO - AAPS PharmSciTech. 2026 Oct 27;27(8):340. doi: 10.1208/s12249-026-03587-0. PMID- 42946929 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1573-2630 (Electronic) IS - 0165-5701 (Linking) VI - 46 IP - 1 DP - 2026 Oct 27 TI - Topographic and layer-specific mapping of anterior scleral complex thickness using anterior segment OCT: an anatomical reference with potential surgical relevance. LID - 442 [pii] LID - 10.1007/s10792-026-04307-0 [doi] AB - PURPOSE: To provide a layer-specific surgical atlas of the anterior scleral complex and link the AS-OCT anatomy to the tissue planes and entry zones used during glaucoma filtration surgery, scleral-fixated IOL implantation, intravitreal injection and ocular surface surgery. METHODS: This prospective cross-sectional study included 120 healthy Turkish volunteers. Thicknesses of the scleral stroma, episclera, Tenon's capsule and conjunctiva were measured at 1, 2, 3 and 4 mm from the scleral spur in four quadrants using wide-angle anterior segment optical coherence tomography with rectus muscle visualization. Measurement reliability was assessed using the ICC, SEM, TEM and repeatability coefficient. RESULTS: The mean age of participants was 42.74 +/- 15.31 years; 72.5% were female. Layer-specific boundaries of all four tissues were feasible, with most parameters demonstrating good to excellent reliability. Notably, certain measurements of the thickness of the medial Tenon's capsule were significantly greater in females. Scleral stromal and episcleral thickness generally increased with age across most quadrants. The thickness of Tenon's capsule generally decreased with age. Conjunctival thickness changes were quadrant-specific, with a significant increases in the inferior and medial quadrants with age. Topographical analysis revealed that scleral stromal thickness progressively decreased toward the periphery, whereas episcleral thickness generally increased. CONCLUSION: Beyond establishing normative AS-OCT measurements, this study introduces anterior scleral anatomy into a potential clinically relevant surgical reference framework. The layer-specific topographical atlas and age-related remodeling patterns describe the tissue characteristics encountered at commonly used surgical locations and may inform surgeons in selecting flap depth, fixation sites, dissection planes and injection zones according to patient age and quadrant-specific anatomy. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature B.V. FAU - Kilinc, Ali Kutay AU - Kilinc AK AUID- ORCID: 0009-0001-2305-7718 AD - Department of Ophthalmology, Gulhane Training and Research Hospital, Merkez Ankara Konutlari (D Blok 134), Ankara, Turkey. akutayk@outlook.com. FAU - Icoz, Mehmet AU - Icoz M AUID- ORCID: 0000-0002-6939-8585 AD - Department of Ophthalmology, Yozgat City Hospital, Yozgat, Turkey. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Int Ophthalmol JT - International ophthalmology JID - 7904294 SB - IM MH - Humans MH - Female MH - *Sclera/anatomy & histology/diagnostic imaging MH - *Tomography, Optical Coherence/methods MH - Prospective Studies MH - Adult MH - Cross-Sectional Studies MH - Male MH - Middle Aged MH - *Anterior Eye Segment/diagnostic imaging/anatomy & histology MH - Reproducibility of Results MH - Young Adult MH - Healthy Volunteers MH - Aged MH - Reference Values MH - Conjunctiva/anatomy & histology MH - *Glaucoma/surgery OTO - NOTNLM OT - Anterior segment optical coherence tomography OT - Conjunctival thickness OT - Normative data OT - Scleral stroma OT - Scleral thickness OT - Tenon's capsule COIS- Declarations. Conflict of interest: The authors declare no competing interests. Ethics approval and consent to participate: Ankara Etlik City Hospital Ethics Committee provided permission (Approval Number: AESH-EK-2025-287) and written informed consent was obtained from all participants involved in the study. Consent for publication: Not Applicable. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 00:24 PHST- 2026/06/10 00:00 [received] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:24 [entrez] AID - 10.1007/s10792-026-04307-0 [pii] AID - 10.1007/s10792-026-04307-0 [doi] PST - epublish SO - Int Ophthalmol. 2026 Oct 27;46(1):442. doi: 10.1007/s10792-026-04307-0. PMID- 42946927 OWN - NLM STAT- Publisher LR - 20261028 IS - 1573-904X (Electronic) IS - 0724-8741 (Linking) DP - 2026 Oct 27 TI - Identity Testing of Biopharmaceuticals using Nano-Differential-Scanning-Fluorimetry. LID - 10.1007/s11095-026-04220-2 [doi] AB - PURPOSE: Identity (ID) testing is an integral part of the analytical control strategy of biopharmaceutical products confirming the ID of both drug substances (DS) before starting the fill-finish process as well as of the finished drug product (DP). Common methods for ID testing, including liquid chromatography-mass spectrometry and immunoassays, often involve time-intensive workflows and demanding data analysis, creating challenges and added complexity for routine quality control ID testing. METHODS: This study evaluated a novel ID testing approach using nano-scale differential scanning fluorimetry (nanoDSF), which measures intrinsic protein fluorescence during thermal unfolding. Multiple data analysis strategies were evaluated, including single-point, curve correlation, and squared Euclidean distance (ED(2)) analyses. RESULTS: A panel of fifteen biopharmaceutical products was tested representing diverse molecular formats. Method performance was assessed using confusion matrices comparing samples to reference profiles. The ED(2) metric (threshold 0.006) demonstrated strongest performance, achieving 100% specificity and 99.7% sensitivity across the product panel excluding biosimilars. CONCLUSION: The nanoDSF method's robustness and rapid turnaround time support its suitability as an ID test for facilities handling multiple products and diverse product types. The selected data analysis approach does not require generation of statistical models enabling simplified method validation and implementation in QC. CI - (c) 2026. The Author(s). FAU - Fakler, Elisabeth AU - Fakler E AD - ten23 health AG, Mattenstr. 22, 4058, Basel, Switzerland. AD - Department of Pharmaceutics, Institute of Pharmaceutical Sciences, University of Freiburg, Sonnenstr. 5, 79104, Freiburg i. Br., Germany. FAU - Kiessig, Steffen AU - Kiessig S AD - ten23 health AG, Mattenstr. 22, 4058, Basel, Switzerland. FAU - Mahler, Hanns-Christian AU - Mahler HC AD - ten23 health AG, Mattenstr. 22, 4058, Basel, Switzerland. FAU - Allmendinger, Andrea AU - Allmendinger A AUID- ORCID: 0000-0002-8250-0978 AD - ten23 health AG, Mattenstr. 22, 4058, Basel, Switzerland. andrea.allmendinger@ten23.health. AD - Department of Pharmaceutics, Institute of Pharmaceutical Sciences, University of Freiburg, Sonnenstr. 5, 79104, Freiburg i. Br., Germany. andrea.allmendinger@ten23.health. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Pharm Res JT - Pharmaceutical research JID - 8406521 SB - IM OTO - NOTNLM OT - analytical method development OT - biopharmaceuticals OT - fluorescence OT - identity testing OT - incoming quality control OT - melting temperature OT - nano differential scanning fluorimetry OT - protein therapeutics OT - thermostability COIS- Declarations. Conflict of interest: All authors are employees of ten23 health. Hanns-Christian Mahler is also advisory board member of NanoTemper Technologies which manufactures the analytical instrument (Prometheus Panta) as well as the corresponding software (PR.Panta Control and PR.Panta Analysis) used in this study. This relationship did not influence the study design, data collection, data interpretation, or conclusions presented in this manuscript. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:24 PHST- 2026/06/01 00:00 [received] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:24 [entrez] AID - 10.1007/s11095-026-04220-2 [pii] AID - 10.1007/s11095-026-04220-2 [doi] PST - aheadofprint SO - Pharm Res. 2026 Oct 27. doi: 10.1007/s11095-026-04220-2. PMID- 42946923 OWN - NLM STAT- Publisher LR - 20261028 IS - 1525-1497 (Electronic) IS - 0884-8734 (Linking) DP - 2026 Oct 27 TI - Anticoagulation and All-Cause Mortality in Adults Aged 80 Years and Older with Atrial Fibrillation: A Retrospective Cohort Study. LID - 10.1007/s11606-026-10920-7 [doi] AB - BACKGROUND: The benefits and risks of anticoagulation therapy in patients aged >/= 80 years with atrial fibrillation (AF) remain underexplored, as this population is underrepresented in randomized clinical trials. We evaluated mortality and bleeding outcomes associated with anticoagulation use in this population. DESIGN: We conducted a retrospective cohort study using MDClone, a synthetic electronic health record database from a large US health system, including 45,305 patients aged >/= 80 years with AF after excluding those with mitral stenosis, mechanical heart valves, cirrhosis, or end-stage renal disease. Patients were categorized as continued, initiated, discontinued, or never anticoagulated. The primary outcome was all-cause mortality at 30 days, 6 months, 1 year, and 5 years; secondary outcomes were ischemic stroke, gastrointestinal bleeding (GIB), and intracranial hemorrhage (ICH). To address immortal time bias, the primary analysis used a time-varying Cox model, complemented by a 90-day landmark analysis and propensity score matching. KEY RESULTS: Patients who continued anticoagulation had lower 1-year mortality than those never anticoagulated (8.7% vs 17.7%; p < 0.001). In the time-varying Cox model, anticoagulation was associated with a 25% lower hazard of mortality (HR 0.75, 95% CI 0.71-0.79). Compared with continued anticoagulation, mortality was higher among patients who initiated (HR 1.51, 95% CI 1.38-1.66), discontinued (HR 1.64, 95% CI 1.49-1.81), or never received anticoagulation (HR 2.22, 95% CI 2.05-2.41), and persisted after propensity score matching. At 5 years, mortality converged across groups. GIB was more frequent in anticoagulated patients (continued 13.0%, initiated 11.9%), whereas ICH rates were low and comparable across groups (1.3-1.6%, p = 0.129). CONCLUSIONS: Among patients aged >/= 80 years with AF, anticoagulation therapy was associated with lower all-cause mortality despite an increase in gastrointestinal bleeding. These findings support continued anticoagulation in appropriately selected elderly patients, rather than withholding therapy on the basis of age alone. CI - (c) 2026. The Author(s). FAU - Kaya, Gizem AU - Kaya G AUID- ORCID: 0009-0006-0998-0342 AD - Division of Hospital Medicine, Barnes Jewish Hospital, Washington University School of Medicine, Saint Louis, MO, USA. gizem@wustl.edu. FAU - Sanchez Garcia, Jonathan AU - Sanchez Garcia J AD - Washington University School of Medicine, Institute for Informatics, Data Science and Biostatistics, Saint Louis, MO, USA. FAU - Schneider, Michelle AU - Schneider M AD - Washington University School of Medicine, Institute for Informatics, Data Science and Biostatistics, Saint Louis, MO, USA. FAU - Yadav, Devvrat AU - Yadav D AD - Division of Hospital Medicine, Barnes Jewish Hospital, Washington University School of Medicine, Saint Louis, MO, USA. FAU - Ndukwe, Miriam AU - Ndukwe M AD - Washington University School of Medicine, Saint Louis, MO, USA. FAU - Chen, Chien-Huan AU - Chen CH AD - Division of Gastroenterology, Washington University School of Medicine, Saint Louis, MO, USA. FAU - Joynt Maddox, Karen E AU - Joynt Maddox KE AD - Division of Cardiology, Washington University School of Medicine, Saint Louis, MO, USA. AD - Center for Advancing Health Services, Policy & Economics Research, Washington University School of Medicine and School of Public Health, Saint Louis, MO, USA. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Gen Intern Med JT - Journal of general internal medicine JID - 8605834 SB - IM OTO - NOTNLM OT - atrial fibrillation OT - geriatric population OT - mortality COIS- Declarations. Human Ethics and Consent to Participate: Not applicable. Consent for Publication: It has not been submitted as a publication to any journal before. Conflicts of interest: The authors have no conflicts of interest. Clinical Trial Number: Not applicable. Disclosures: Dr. Joynt Maddox receives research support from the National Heart, Lung, and Blood Institute (R01HL143421 and R01HL164561), National Institute of Nursing Research (U01NR020555), National Institute on Aging (R01AG089215), National Center for Advancing Translational Sciences (UL1TR002345), Schmidt Futures, Arnold Foundation, and Missouri Foundation for Health. She serves as an Associate Editor for the Journal of the American Medical Association (JAMA). The remaining authors have no disclosures. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:24 PHST- 2026/02/03 00:00 [received] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:24 [entrez] AID - 10.1007/s11606-026-10920-7 [pii] AID - 10.1007/s11606-026-10920-7 [doi] PST - aheadofprint SO - J Gen Intern Med. 2026 Oct 27. doi: 10.1007/s11606-026-10920-7. PMID- 42946922 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1437-2320 (Electronic) IS - 0344-5607 (Linking) VI - 49 IP - 1 DP - 2026 Oct 28 TI - When age matters: mortality and disability after aneurysmal subarachnoid hemorrhage in a cohort of elderly patients. LID - 625 [pii] LID - 10.1007/s10143-026-04546-w [doi] AB - Aneurysmal subarachnoid hemorrhage (aSAH) in older adults is associated with high early mortality and substantial long-term disability. As referral patterns and neurocritical care have evolved, the age at which prognosis changes markedly remains uncertain, and longitudinal functional trajectories beyond discharge are poorly characterised in elderly patients. We retrospectively included consecutive patients aged 65 years or older admitted with confirmed aSAH to the National Hospital for Neurology and Neurosurgery (London, UK) between February 2013 and August 2022. Primary outcomes were in-hospital mortality, all-cause mortality during follow-up, and functional disability (modified Rankin Scale, mRS) at discharge and last follow-up (unfavourable outcome defined as mRS 4-6). Age thresholds associated with mortality and disability were identified using receiver operating characteristic analysis with the Youden index and confirmed by maximally selected rank statistics. Predictors of in-hospital death, survival, and disability were evaluated using multivariable logistic regression and Cox proportional hazards models. Functional trajectories were summarised using transition matrices and Sankey diagrams. Among 110 patients (median age 76 years; 80% women), in-hospital mortality was 18.2% and all-cause mortality during follow-up was 48.2% (median follow-up 24 months). Age independently predicted in-hospital death (odds ratio 1.19 per year, 95% CI 1.06-1.35) and long-term mortality (hazard ratio 1.16 per year, 95% CI 1.09-1.22). An exploratory, cohort-derived age cut point of 79.5 years delineated divergent trajectories: mortality increased from 10.4% in patients aged < 80 years to 36.4% in those aged >/= 80 years, and median survival declined from 95 months to 3 months (log-rank p = 2.8 x 10(-)(6)). Among patients discharged alive, discharge mRS independently predicted post-discharge survival (hazard ratio 2.39 per point, 95% CI 1.60-3.56), including in those aged >/= 80 years (hazard ratio 3.34, 95% CI 1.21-9.20). Functional trajectories showed frequent post-discharge recovery in patients aged < 80 years, whereas recovery was uncommon and progression to death frequent in those aged >/= 80 years. In this cohort of elderly patients with aSAH, an exploratory, cohort-derived threshold of approximately 80 years identified patients with markedly lower survival and functional recovery. Longitudinal trajectory analysis suggests post-discharge improvement remains common in patients aged < 80 years but rare in those aged >/= 80 years. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Fiore, Giorgio AU - Fiore G AUID- ORCID: 0000-0003-4550-3675 AD - Victor Horsley Department of Neurosurgery, National Hospital for Neurology and Neurosurgery, London, WC1N 3BG, UK. giorgiofiore.doc@gmail.com. AD - Unit of Neurosurgery, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, 20122, Italy. giorgiofiore.doc@gmail.com. AD - UCL Queen Square Institute of Neurology, London, WC1N 3BG, UK. giorgiofiore.doc@gmail.com. AD - Department of Pathophysiology and Transplantation, University of Milan, Milan, 20122, Italy. giorgiofiore.doc@gmail.com. FAU - Reischer, Gerda AU - Reischer G AD - Victor Horsley Department of Neurosurgery, National Hospital for Neurology and Neurosurgery, London, WC1N 3BG, UK. FAU - Kovacs, Bernadett AU - Kovacs B AD - Victor Horsley Department of Neurosurgery, National Hospital for Neurology and Neurosurgery, London, WC1N 3BG, UK. FAU - Murphy, Mary AU - Murphy M AD - Victor Horsley Department of Neurosurgery, National Hospital for Neurology and Neurosurgery, London, WC1N 3BG, UK. FAU - Miserocchi, Anna AU - Miserocchi A AD - Victor Horsley Department of Neurosurgery, National Hospital for Neurology and Neurosurgery, London, WC1N 3BG, UK. FAU - Castanho, Pedro AU - Castanho P AD - Victor Horsley Department of Neurosurgery, National Hospital for Neurology and Neurosurgery, London, WC1N 3BG, UK. pedro.castanho@nhs.net. LA - eng PT - Journal Article DEP - 20261028 PL - Germany TA - Neurosurg Rev JT - Neurosurgical review JID - 7908181 SB - IM MH - Humans MH - *Subarachnoid Hemorrhage/mortality/surgery MH - Female MH - Male MH - Aged MH - Retrospective Studies MH - Aged, 80 and over MH - Age Factors MH - Hospital Mortality MH - Prognosis MH - Cohort Studies OTO - NOTNLM OT - Aged OT - Aneurysm OT - Functional outcome OT - Mortality OT - Subarachnoid hemorrhage OT - Survival COIS- Declarations. Ethics approval: The study was approved by the institutional review board as a service evaluation, and the requirement for informed consent was waived owing to its retrospective nature. Disclosures: The authors report no conflicts of interest. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 00:24 PHST- 2026/06/08 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:24 [entrez] AID - 10.1007/s10143-026-04546-w [pii] AID - 10.1007/s10143-026-04546-w [doi] PST - epublish SO - Neurosurg Rev. 2026 Oct 28;49(1):625. doi: 10.1007/s10143-026-04546-w. PMID- 42946919 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1862-3514 (Electronic) VI - 21 IP - 1 DP - 2026 Oct 27 TI - Revisiting menopausal hormone therapy for bone health: evidence, controversies, and clinical positioning in India in 2026. LID - 152 [pii] LID - 10.1007/s11657-026-01792-9 [doi] AB - Menopause is characterized by the rapid decline of estrogen exposure, resulting in accelerated bone loss superimposed on the gradual age-related decline in bone mass. This process is particularly relevant in India, where women experience menopause earlier than Western populations, thereby increasing lifetime fracture risk. Menopausal hormone therapy (MHT) has been evaluated extensively over the years for skeletal health, with consistent evidence demonstrating preservation of bone mineral density and reduction in vertebral and hip fractures, especially when initiated early after menopause. Although multiple concerns arose following the Women's Health Initiative in terms of cardiovascular safety and breast cancer risk, leading to a decline in MHT use, subsequent analyses have supported a more favorable benefit-risk profile in appropriately selected younger postmenopausal women. Current evidence suggests that individualized MHT can also improve skeletal outcomes while benefiting menopausal symptoms and overall quality of life. This review revisits the evolving role of MHT in bone health in the Indian context. CI - (c) 2026. The Author(s), under exclusive licence to the International Osteoporosis Foundation and the Bone Health and Osteoporosis Foundation. FAU - Vaghasia, Nupoor AU - Vaghasia N AD - Department of Endocrinology, Max Super Specialty Hospital, New Delhi, India. Nupoor11@gmail.com. FAU - Nagendra, Lakshmi AU - Nagendra L AD - Department of Endocrinology, JSS Medical College, JSS Academy of Higher Education and Research, Mysore, India. FAU - Kapoor, Nitin AU - Kapoor N AD - Department of Endocrinology, Diabetes and Metabolism, Christian Medical College, Vellore, India. FAU - Maldar, Aasim AU - Maldar A AD - Department of Endocrinology, P. D. Hinduja National Hospital and Medical Research Centre, Mumbai, India. FAU - Baro, Abhamoni AU - Baro A AD - Department of Endocrinology, Gauhati Medical College and Hospital, Guwahati, India. FAU - Bhandarkar, Akhila AU - Bhandarkar A AD - Department of Endocrinology and Metabolism, Nitte (Deemed to be university) KS Hegde Medical Academy (KSHEMA), Mangalore, India. FAU - Joshi, Ameya AU - Joshi A AD - Department of Endocrinology, Bhaktivedanta Hospital And Research Institute, Thane, India. FAU - Ghosh, Amritava AU - Ghosh A AD - Department of Endocrinology, All India Institute of Medical Sciences, Raipur, India. FAU - Monteiro, Ana S AU - Monteiro AS AD - Department of Endocrinology, Goa Medical College, Goa, India. FAU - Joseph, Aneez AU - Joseph A AD - Department of Endocrinology and Metabolism, Malankara Orthodox Syrian Church Medical College, Kolenchery, India. FAU - Mishra, Ipsita AU - Mishra I AD - Department of Endocrinology and Metabolism, Institute of Medical Sciences & SUM Hospital, Institute of Medical Sciences & SUM Hospital, Phulnakhara, Cuttack, India. FAU - Agarwal, Khushboo AU - Agarwal K AD - Department of Endocrinology, Diabetes and Metabolism, Christian Medical College, Vellore, India. FAU - Cherian, Kripa E AU - Cherian KE AD - Department of Endocrinology, Diabetes and Metabolism, Christian Medical College, Vellore, India. FAU - Tandon, Nikhil AU - Tandon N AD - Department of Endocrinology & Metabolism, All India Institute of Medical Sciences, New Delhi, India. FAU - Kaur, Parjeet AU - Kaur P AD - Department of Endocrinology and Diabetes, Medanta Hospital, Gurugram, India. FAU - John, Rebecca AU - John R AD - Department of Endocrinology, Diabetes and Metabolism, Christian Medical College, Vellore, India. FAU - Shetty, Sahana AU - Shetty S AD - Department of Endocrinology, Kasturba Medical College, Manipal, India. FAU - Das, Sambit AU - Das S AD - Department of Endocrinology, Kalinga Institute of Medical Sciences, Bhubaneswar, India. FAU - Bhadada, Sanjay AU - Bhadada S AD - Department of Endocrinology, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India. FAU - Kalra, Sanjay AU - Kalra S AD - Department of Endocrinology, Bharti Hospital, Karnal, India. FAU - Bhattacharya, Saptarshi AU - Bhattacharya S AD - Department of Endocrinology, Indraprastha Apollo Hospital, Delhi, India. FAU - Patra, Shinjan AU - Patra S AD - Department of Endocrinology and Metabolism, All India Institute of Medical Sciences, Kalyani, India. FAU - Sidana, Shivani AU - Sidana S AD - Department of Endocrinology and Metabolism, All India Institute of Medical Sciences, Bathinda, India. FAU - Mondal, Sunetra AU - Mondal S AD - Department of Endocrinology, Nil Ratan Sarkar Medical College, Kolkat, India. FAU - Paul, Thomas V AU - Paul TV AD - Department of Endocrinology, Diabetes and Metabolism, Christian Medical College, Vellore, India. FAU - Sriram, Usha AU - Sriram U AD - Department of Diabetes and Endocrinology, The Voluntary Health Services, Chennai, India. FAU - Mithal, Ambrish AU - Mithal A AD - Department of Endocrinology, Max Super Specialty Hospital, New Delhi, India. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - England TA - Arch Osteoporos JT - Archives of osteoporosis JID - 101318988 SB - IM MH - Humans MH - Female MH - India MH - *Osteoporosis, Postmenopausal/prevention & control MH - Bone Density/drug effects MH - *Estrogen Replacement Therapy MH - *Menopause/physiology MH - Osteoporotic Fractures/prevention & control OTO - NOTNLM OT - Bone health OT - Fracture risk OT - Indian women OT - Menopausal hormone therapy OT - Postmenopausal osteoporosis COIS- Declarations. Conflict of interest: NV has no conflicts of interest to declare related to this work; LN has no conflicts of interest to declare related to this work; NK has no conflicts of interest to declare related to this work; AM has no conflicts of interest to declare related to this work; AB has no conflicts of interest to declare related to this work; AB has no conflicts of interest to declare related to this work; AJ has no conflicts of interest to declare related to this work; AG has no conflicts of interest to declare related to this work; ASM has no conflicts of interest to declare related to this work; AJ has no conflicts of interest to declare related to this work; IMa has no conflicts of interest to declare related to this work; KA has no conflicts of interest to declare related to this work; KEC has no conflicts of interest to declare related to this work; NT has no conflicts of interest to declare related to this work; PK has no conflicts of interest to declare related to this work; RJ has no conflicts of interest to declare related to this work; SS has no conflicts of interest to declare related to this work; SD has no conflicts of interest to declare related to this work; SB has no conflicts of interest to declare related to this work; SK has no conflicts of interest to declare related to this work; SB has no conflicts of interest to declare related to this work; SP has no conflicts of interest to declare related to this work; SS has no conflicts of interest to declare related to this work; SM has no conflicts of interest to declare related to this work; TVP has no conflicts of interest to declare related to this work; US has no conflicts of interest to declare related to this work; AM has no conflicts of interest to declare related to this work. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 00:23 PHST- 2026/07/16 00:00 [received] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:23 [entrez] AID - 10.1007/s11657-026-01792-9 [pii] AID - 10.1007/s11657-026-01792-9 [doi] PST - epublish SO - Arch Osteoporos. 2026 Oct 27;21(1):152. doi: 10.1007/s11657-026-01792-9. PMID- 42946912 OWN - NLM STAT- Publisher LR - 20261028 IS - 1531-135X (Electronic) IS - 1530-7026 (Linking) DP - 2026 Oct 27 TI - Large nesting in deer mice co-occurs with jumping but not running stereotypies: Insights into compulsive-like behavioural heterogeneity. LID - 10.3758/s13415-026-01510-5 [doi] AB - Subpopulations of deer mice present with distinct compulsive-like behavioural phenotypes, i.e., large nesting (large nest-building [LNB]) and high motor stereotypy (HS). Large nest-building and HS associate with unique dopaminergic and adenosinergic mechanisms. Large nest-building is also characterized by increased risk-taking behaviour, which has not yet been shown in HS mice. We hypothesised that LNB and HS represent distinct compulsive-like outcomes that would not manifest in the same subjects. We also hypothesised that LNB and HS would each respond uniquely to dopamine D(1) receptor modulation, confirming distinct neurobiological profiles that could guide future studies aimed at enhancing treatment outcomes. Briefly, 267 mice were screened for nesting behaviour, of which 36 mice were selected for normal nesting (normal nest-building [NNB]) and 44 for LNB. Mice were subsequently exposed to either control injections or to the highly selective D(1) agonist (SKF-38393), D(1) antagonist (ecopipam), or the A(1) agonist (capadenoson) for 32 days. Mice were then retested for nesting and stereotypy expression and risk-prone open field behaviour. We show that LNB is associated with lower overall motor stereotypical expression compared with NNB. We also show that vertical jumping co-occurs with both NNB and LNB behaviour, whereas pattern running is only shown by NNB mice and that neither LNB nor HS was impacted by selective D(1) receptor modulation. Collectively, these results strengthen earlier observations in deer mice with respect to compulsive-like behavioural heterogeneity and provide a solid foundation for research into the aetiology of equally persistent and repetitive but unique compulsive-like behavioural phenotypes. CI - (c) 2026. The Author(s). FAU - van Staden, Anmarie AU - van Staden A AD - Centre of Excellence for Pharmaceutical Sciences, Department of Pharmacology, Faculty of Health Sciences, North-West University, Potchefstroom, South Africa. FAU - Sampson, Timothy R AU - Sampson TR AD - Department of Cell Biology, Emory School of Medicine, Atlanta, GA, USA. FAU - Lochner, Christine AU - Lochner C AD - Department of Psychiatry, SAMRC Unit on Risk and Resilience in Mental Disorders, Stellenbosch University, Cape Town, South Africa. FAU - Wolmarans, De Wet AU - Wolmarans W AUID- ORCID: 0000-0002-3440-4869 AD - Centre of Excellence for Pharmaceutical Sciences, Department of Pharmacology, Faculty of Health Sciences, North-West University, Potchefstroom, South Africa. dewet.wolmarans@nwu.ac.za. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Cogn Affect Behav Neurosci JT - Cognitive, affective & behavioral neuroscience JID - 101083946 SB - IM OTO - NOTNLM OT - Adenosine OT - Compulsivity OT - Deer mouse OT - Dopamine OT - Nest building OT - Risk-taking OT - Stereotypy COIS- Declarations. Ethics approval: The ethics governing the use and conduct of experiments on animals were strictly observed, and the experimental protocol was approved by the North-West University AnimCare Research Ethics Committee (approval number: NWU-00788-24-A5; committee registration Nr. AREC-130913-015). All procedures were carried out in accordance with the rules and guidelines stipulated by the South African National Standard 10386:2021 "The care and use of animals for scientific purposes." Consent to participate: Not applicable. Consent for publication: Not applicable. Conflicts of interests: The authors have no financial or proprietary interests in any material discussed in this article. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:23 PHST- 2026/04/10 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:23 [entrez] AID - 10.3758/s13415-026-01510-5 [pii] AID - 10.3758/s13415-026-01510-5 [doi] PST - aheadofprint SO - Cogn Affect Behav Neurosci. 2026 Oct 27. doi: 10.3758/s13415-026-01510-5. PMID- 42946908 OWN - NLM STAT- Publisher LR - 20261028 IS - 1573-2622 (Electronic) IS - 0012-4486 (Linking) DP - 2026 Oct 27 TI - Impact of Anti-VEGF treatment on retinal anatomy and electrophysiological function in diabetic macular edema. LID - 10.1007/s10633-026-10163-1 [doi] AB - PURPOSE: The objective of this study is to evaluate changes in the foveal avascular zone (FAZ), vascular density (VD), and electrophysiological parameters in patients with diabetic macular edema (DME) following intravitreal bevacizumab therapy. METHODS: The study comprised 27 eyes of 22 patients with treatment-naive DME who received a loading phase of three consecutive monthly intravitreal bevacizumab injections, together with 30 eyes of 15 healthy controls. All participants underwent optical coherence tomography (OCT), OCT angiography (OCTA), pattern electroretinography (PERG) and visual evoked potentials (VEP) at baseline and at one and three months. As both eyes of some participants were included, all comparisons used generalized estimating equations clustered on subject, with cluster-robust variance estimation. RESULTS: Central retinal thickness decreased significantly after treatment, from 409.2 +/- 105.9 microm at baseline to 368.4 +/- 97.8 microm at 1 month (p = 0.007) and 352.6 +/- 93.6 microm at 3 months (p < 0.001). Best-corrected visual acuity improved from 0.69 +/- 0.47 to 0.57 +/- 0.44 logMAR, but this change did not reach statistical significance (p = 0.166). FAZ measurements showed no significant change at either visit (all p > 0.57) and superficial VD remained stable (p = 0.404 and p = 0.185), whereas measured deep VD increased significantly at both 1 month (+ 1.02%, p = 0.045) and 3 months (+ 1.85%, p = 0.002), a change that may reflect improved OCTA signal penetration after edema resolution rather than true reperfusion. The 100% contrast response magnitude increased at 3 months (p = 0.040) but not at 1 month (p = 0.293), and did not retain significance after adjustment for multiple comparisons. Lc and Hc amplitudes increased significantly at 3 months (p = 0.021 and p < 0.001), whereas latencies remained prolonged relative to controls at baseline (Lc, p = 0.001; Hc, p = 0.004). CONCLUSION: Intravitreal bevacizumab therapy in treatment-naive DME patients produced significant anatomical improvement, accompanied by changes in selected electrophysiological parameters. The stability of FAZ measurements, the apparent increase in deep VD, and the improved VEP amplitudes, in the absence of concurrent normalisation of latency, suggest that structural, microvascular and functional recovery may follow different time courses. This indicates that anatomical response alone may overestimate functional benefit. The electrophysiological changes observed were modest and require confirmation in larger cohorts. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Akay Salihoglu, Doga AU - Akay Salihoglu D AUID- ORCID: 0000-0001-7581-594X AD - Department of Ophthalmology, Antalya Training and Research Hospital, Varlik Mahallesi, 07070, Antalya, Turkey. dogaakay93@gmail.com. FAU - Dogan, Mehmet Erkan AU - Dogan ME AUID- ORCID: 0000-0002-8474-4861 AD - Department of Ophthalmology, Faculty of Medicine, Akdeniz University, Antalya, Turkey. FAU - Akar, Yusuf AU - Akar Y AUID- ORCID: 0009-0006-1365-2923 AD - Department of Ophthalmology, Faculty of Medicine, Akdeniz University, Antalya, Turkey. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Doc Ophthalmol JT - Documenta ophthalmologica. Advances in ophthalmology JID - 0370667 SB - IM OTO - NOTNLM OT - Bevacizumab OT - Foveal avascular zone OT - Optical coherence tomography angiography OT - Pattern electroretinography OT - Vessel density OT - Visual evoked potentials COIS- Declarations. Conflict of interest: The authors declare no competing interests. Ethical approval: This study was approved by the Ethics Committee of the Faculty of Medicine (Approval Number 2012-KAEK-20). All procedures performed in studies involving human participants were conducted in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Statement of Human Rights: All procedures involving human participants were conducted in accordance with the ethical standards of the institutional research ethics committee and the principles of the Declaration of Helsinki. Statement on the Welfare of Animals: No animals were involved in this study. Informed Consent: Written informed consent was obtained from all participants included in the study. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:23 PHST- 2026/04/28 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:23 [entrez] AID - 10.1007/s10633-026-10163-1 [pii] AID - 10.1007/s10633-026-10163-1 [doi] PST - aheadofprint SO - Doc Ophthalmol. 2026 Oct 27. doi: 10.1007/s10633-026-10163-1. PMID- 42946901 OWN - NLM STAT- Publisher LR - 20261028 IS - 1573-2584 (Electronic) IS - 0301-1623 (Linking) DP - 2026 Oct 27 TI - Focal therapy vs radical prostatectomy for localized prostate cancer: a systematic review, meta-analysis, bayesian network, and trial sequential analysis. LID - 10.1007/s11255-026-05466-w [doi] AB - BACKGROUND: Prostate cancer is a prevalent malignancy with procedural treatment strategies. These treatments are associated with significant quality of life impact, including erectile dysfunction and urinary incontinence. Focal therapy (FT) has emerged as an option that may minimize treatment-related side effects. Its efficacy in cancer control compared to standard strategies, such as radical prostatectomy (RP), remains controversial. This systematic review and meta-analysis sought to compare the oncologic and functional outcomes of focal therapy versus radical prostatectomy (RP) for localized prostate cancer. MATERIALS AND METHODS: A comprehensive systematic search was conducted across PubMed, EMBASE, Scopus, and Google Scholar in October 2025. The study followed PRISMA guidelines and included prospective and retrospective observational studies comparing RP with FT. Eleven studies met inclusion criteria providing a total of 8399 patients of which 4498 underwent RP and 3901 underwent FT. The coprimary endpoints were salvage treatment-free survival and failure-free survival, with secondary endpoints including continence, erectile function, and complication rate. RESULTS: Oncologic outcomes revealed a statistically significant overall difference in salvage treatment-free survival and failure-free survival between FT and RP, with a HR of 2.05 (95% Cl 1.29-3.26, p=0.002) and 2.68 (95% CI 1.04-6.93, p=0.04), respectively. Differences in overall survival were not statistically significant HR 1.71 (95% CI 0.75-3.90, p = 0.20). Subgroup analyses showed varied results across different focal therapy approaches, with cryotherapy and combined approaches demonstrating higher treatment failure rates. Functional outcomes strongly favored FT, with significantly higher rates of continence and erectile function across all measured time intervals. At 12 months post-treatment, FT patients demonstrated substantially better continence (OR 3.45, p<0.00001) and erectile function preservation (OR 6.43, p<0.00001) compared to RP. CONCLUSION: FT offers substantial quality of life benefits, particularly within the first 12 postoperative months, compared to RARP, however, at the cost of decreased oncologic treatment effectiveness. Differences in oncologic outcomes become attenuated in propensity-matched studies and trial sequential analysis yielded inconclusive results. Careful patient selection is paramount, and patients should be thoroughly counseled about potentially reduced oncologic control effectiveness and increased post-ablation monitoring. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature B.V. FAU - Hinojosa-Gonzalez, David E AU - Hinojosa-Gonzalez DE AUID- ORCID: 0000-0002-0233-0607 AD - Scott Department of Urology, Baylor College of Medicine, 7200 Cambridge St, Houston, TX, 77030, USA. David.hinojosa-gonzalez@bcm.edu. FAU - Slawin, Bailey AU - Slawin B AD - UTHealth, McGovern School of Medicine, Houston, TX, USA. FAU - Torres-Martinez, Mauricio AU - Torres-Martinez M AD - Department of International Health, Hopkins Bloomberg School of Public Health, Baltimore, MD, USA. FAU - Coady, Peyton J AU - Coady PJ AD - Scott Department of Urology, Baylor College of Medicine, 7200 Cambridge St, Houston, TX, 77030, USA. FAU - Hernandez, Beatriz S AU - Hernandez BS AD - Scott Department of Urology, Baylor College of Medicine, 7200 Cambridge St, Houston, TX, 77030, USA. FAU - Calvillo-Ramirez, Alejandro AU - Calvillo-Ramirez A AD - Department of Surgery Section of Urology, University of Chicago, Chicago, IL, USA. FAU - Saffati, Gal AU - Saffati G AD - Scott Department of Urology, Baylor College of Medicine, 7200 Cambridge St, Houston, TX, 77030, USA. FAU - Oppenheimer, Amelia G AU - Oppenheimer AG AD - Scott Department of Urology, Baylor College of Medicine, 7200 Cambridge St, Houston, TX, 77030, USA. FAU - de Jesus Aguirre-Vera, Guillermo AU - de Jesus Aguirre-Vera G AD - Tecnologico de Monterrey, Escuela de Medicina y Ciencias de La Salud, Monterrey, Mexico. FAU - Jones, Jeffrey A AU - Jones JA AD - Scott Department of Urology, Baylor College of Medicine, 7200 Cambridge St, Houston, TX, 77030, USA. AD - Michael E. DeBakey VA Medical Center, Houston, TX, USA. FAU - Slawin, Jeremy R AU - Slawin JR AD - Scott Department of Urology, Baylor College of Medicine, 7200 Cambridge St, Houston, TX, 77030, USA. AD - Michael E. DeBakey VA Medical Center, Houston, TX, USA. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Int Urol Nephrol JT - International urology and nephrology JID - 0262521 SB - IM OTO - NOTNLM OT - Cryoablation OT - Focal therapy OT - High-intensity focused ultrasound OT - Robotic-assisted laparoscopic surgery COIS- Declarations. Conflict of interest: This study complied with PROSPERO (CRD42024629679) guidelines. The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request. The authors declare that they have no competing interests. No funding was received for this study. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:23 PHST- 2026/05/27 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:23 [entrez] AID - 10.1007/s11255-026-05466-w [pii] AID - 10.1007/s11255-026-05466-w [doi] PST - aheadofprint SO - Int Urol Nephrol. 2026 Oct 27. doi: 10.1007/s11255-026-05466-w. PMID- 42946897 OWN - NLM STAT- Publisher LR - 20261028 IS - 1463-1326 (Electronic) IS - 1462-8902 (Linking) DP - 2026 Oct 27 TI - Cardiovascular Effects of GLP-1 Receptor Agonists Across Age Groups: An Updated Meta-Analysis of Randomized Controlled Trials. LID - 10.1111/dom.71496 [doi] AB - AIMS: To evaluate whether cardiovascular effects of glucagon-like peptide-1 receptor agonists (GLP-1RAs) differ by age. MATERIALS AND METHODS: PubMed and Embase were searched to 1 July 2026 for randomized placebo-controlled GLP-1RA trials in adults with type 2 diabetes or overweight/obesity reporting age-stratified major adverse cardiovascular events (MACE; a composite of cardiovascular death, non-fatal myocardial infarction, and non-fatal stroke). Hazard ratios (HRs) for participants aged < 65 and >/= 65 years were pooled using random-effects models with restricted maximum likelihood estimation and Hartung-Knapp adjustment. Effect modification was assessed by pooling trial-specific ratios of HRs (RHRs) for >/= 65 versus < 65 years. Where reported, effects were assessed across < 65, 65 to < 75 and >/= 75 years. RESULTS: Eight trials (SUSTAIN-6, EXSCEL, Harmony Outcomes, PIONEER 6, REWIND, AMPLITUDE-O, SELECT and SOUL) comprising 71 926 participants were included. GLP-1RAs reduced MACE at < 65 years and >/= 65 years, with pooled HRs of 0.81 (95% CI 0.69-0.94; I(2) = 64.1%) and 0.84 (95% CI 0.79-0.90; I(2) = 0.0%), respectively. The pooled RHR showed no clear evidence of effect modification (1.03, 95% CI 0.85-1.25), although between-trial heterogeneity was high (I(2) = 59.3%). Among trials reporting three age strata, RHRs showed no clear evidence of effect modification for 65 to < 75 or >/= 75 years versus < 65 years, but data were available from only four trials, and the >/= 75-year group was relatively small. CONCLUSIONS: Across the included trial populations, GLP-1RAs lowered MACE in the age groups examined, with no clear evidence that relative cardiovascular effects differed by age. CI - (c) 2026 The Author(s). Diabetes, Obesity and Metabolism published by John Wiley & Sons Ltd. FAU - Fujii, Ai AU - Fujii A AUID- ORCID: 0000-0002-0904-0127 AD - Canada Excellence Research Chair Program in Genomic Medicine, McGill University, Montreal, Quebec, Canada. AD - Department of Nephrology, Kanazawa Medical University, Uchinada, Ishikawa, Japan. FAU - Hasebe, Masashi AU - Hasebe M AUID- ORCID: 0000-0001-7031-2446 AD - Canada Excellence Research Chair Program in Genomic Medicine, McGill University, Montreal, Quebec, Canada. AD - Department of Human Genetics, McGill University, Montreal, Quebec, Canada. AD - Department of Diabetes, Endocrinology and Nutrition, Kyoto University Graduate School of Medicine, Kyoto, Japan. AD - Department of Diabetes and Endocrinology, Medical Research Institute KITANO HOSPITAL, PIIF Tazuke-Kofukai, Osaka, Japan. FAU - Furuichi, Kengo AU - Furuichi K AUID- ORCID: 0000-0002-7069-0938 AD - Department of Nephrology, Kanazawa Medical University, Uchinada, Ishikawa, Japan. FAU - Yabe, Daisuke AU - Yabe D AUID- ORCID: 0000-0002-5334-7687 AD - Department of Diabetes, Endocrinology and Nutrition, Kyoto University Graduate School of Medicine, Kyoto, Japan. FAU - Yoshiji, Satoshi AU - Yoshiji S AUID- ORCID: 0000-0001-8863-2413 AD - Canada Excellence Research Chair Program in Genomic Medicine, McGill University, Montreal, Quebec, Canada. AD - Department of Human Genetics, McGill University, Montreal, Quebec, Canada. LA - eng GR - CRC-2025-00097/Canada Research Chairs Program/ GR - Japan Society for the Promotion of Science/ GR - 183596/CAPMC/CIHR/Canada GR - DNA to RNA (D2R) Foundational Program/ GR - McGill University/ PT - Journal Article DEP - 20261027 PL - England TA - Diabetes Obes Metab JT - Diabetes, obesity & metabolism JID - 100883645 SB - IM OTO - NOTNLM OT - GLP-1 receptor agonists OT - age OT - cardiovascular disease OT - meta-analysis OT - older adults EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:23 PHST- 2026/10/08 00:00 [revised] PHST- 2026/08/10 00:00 [received] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:23 [entrez] AID - 10.1111/dom.71496 [doi] PST - aheadofprint SO - Diabetes Obes Metab. 2026 Oct 27. doi: 10.1111/dom.71496. PMID- 42946891 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1432-1335 (Electronic) IS - 0171-5216 (Print) IS - 0171-5216 (Linking) VI - 152 IP - 10 DP - 2026 Jul 31 TI - Artificial intelligence (AI) in melanocytic lesions diagnosis: a systematic review of methods incorporating ABCD(E)-derived features. LID - 10.1007/s00432-026-06519-4 [doi] LID - 207 AB - Melanoma has a growing incidence, and early and accurate detection can enhance survival rates. However, its complex diagnoses pose challenges that could be overcome by Artificial Intelligence (AI), and the ABCD(E) rule (Asymmetry, Border irregularity, Color variegation, Diameter, and Evolution) is a major screening pattern enabling melanoma detection. This systematic review assessed advancements in Artificial Intelligence (AI) for melanoma diagnosis, particularly focusing on systems incorporating or informed by ABCD(E) criteria. Guided by Cochrane and PRISMA guidelines, this review included experimental or observational studies indexed on PubMed, LILACS, Latindex, and Cochrane Central Register of Controlled Trials platforms. Independent screening and data extraction were performed by two authors. 2,735 records were assessed and 21 studies were included in the final screening, analyzing over 90,000 images/cases. AI systems, notably CNNs and DCNNs, demonstrated promising diagnostic performance, often matching or exceeding human expert evaluation, achieving an Area Under the Curve (AUC) of 90.3% for early melanoma differentiation. Patient selection bias was observed, limiting applicability of the findings. AI shows significant and evolving capability in skin lesion diagnosis, increasingly integrating ABCD(E) criteria. While offering enhanced diagnostic consistency, challenges persist, including data limitations, model generalizability, and interpretability. CI - (c) 2026. The Author(s). FAU - Lopes, Otto Muller Silva AU - Lopes OMS AUID- ORCID: 0009-0005-9300-1487 AD - Federal University of Minas Gerais, Av. Prof. Alfredo Balena, 190 - Santa Efigenia, Belo Horizonte, Minas Gerais, 30130-100, Brazil. otto.plastica@gmail.com. FAU - Oliveira, Juliana Almeida AU - Oliveira JA AUID- ORCID: 0000-0002-4704-2318 AD - Federal University of Minas Gerais, Av. Prof. Alfredo Balena, 190 - Santa Efigenia, Belo Horizonte, Minas Gerais, 30130-100, Brazil. FAU - Nogueira, Victor Brunno Alves AU - Nogueira VBA AUID- ORCID: 0009-0000-7749-8781 AD - Universidade Jose do Rosario Vellano, Belo Horizonte, Minas Gerais, Brazil. FAU - Ribeiro, Ludmila Fausto AU - Ribeiro LF AUID- ORCID: 0009-0009-6703-3775 AD - Federal University of Minas Gerais, Av. Prof. Alfredo Balena, 190 - Santa Efigenia, Belo Horizonte, Minas Gerais, 30130-100, Brazil. FAU - Zamae, Mara Ines Stefanini AU - Zamae MIS AUID- ORCID: 0009-0009-2376-2618 AD - Instituto Nacional do Cancer, Rio de Janeiro, Brazil. FAU - Vieira, Matheus Figueiredo Garrocho Ottoni AU - Vieira MFGO AUID- ORCID: 0009-0005-5685-8270 AD - Universidade Jose do Rosario Vellano, Belo Horizonte, Minas Gerais, Brazil. FAU - Dantas E Pimenta, Vinicius Marciano AU - Dantas E Pimenta VM AUID- ORCID: 0009-0000-5865-2595 AD - Faculdade de Ciencias Medicas de Ipatinga, Ipatinga, Minas Gerais, Brazil. FAU - Reis, Zilma Silveira Nogueira AU - Reis ZSN AUID- ORCID: 0000-0001-6374-9295 AD - Department of Gynecology and Obstetrics, Federal University of Minas Gerais, Belo Horizonte, Brazil. LA - eng PT - Journal Article PT - Review PT - Systematic Review DEP - 20260731 PL - Germany TA - J Cancer Res Clin Oncol JT - Journal of cancer research and clinical oncology JID - 7902060 SB - IM MH - Humans MH - *Melanoma/diagnosis/pathology MH - *Artificial Intelligence MH - *Skin Neoplasms/diagnosis/pathology PMC - PMC13713948 OTO - NOTNLM OT - ABCDE OT - Artificial intelligence OT - Diagnostic measures OT - Melanoma COIS- Declarations. Conflict of interest: The authors declare no competing interests. Clinical trial registration: Does not apply for systematic reviews and metaanalysis. Ethics approval: Does not apply for systematic reviews and meta-analysis. International Prospective Register of Systematic Reviews (PROSPERO) registry: CRD420251151405. Patient consent: Does not apply for systematic reviews and meta-analysis. Permission to reproduce material from other sources: Does not apply for systematic reviews and meta-analysis. No content was reproduced. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:23 PHST- 2026/04/23 00:00 [received] PHST- 2026/05/21 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:23 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1007/s00432-026-06519-4 [pii] AID - 6519 [pii] AID - 10.1007/s00432-026-06519-4 [doi] PST - epublish SO - J Cancer Res Clin Oncol. 2026 Jul 31;152(10):207. doi: 10.1007/s00432-026-06519-4. PMID- 42946884 OWN - NLM STAT- Publisher LR - 20261028 IS - 1741-0444 (Electronic) IS - 0140-0118 (Linking) DP - 2026 Oct 28 TI - Uncertainty-Aware Single-Channel Surface EMG Classification for Low-Resource Hand Gesture Recognition: A Subject-Independent Evaluation. LID - 10.1007/s11517-026-03704-y [doi] AB - Single-channel surface electromyography (sEMG) could reduce the cost and complexity of hand-gesture interfaces, but performance commonly degrades when models are tested on unseen subjects. We evaluated five-class hand gesture classification using only the Flexor Carpi Radialis (FCR) channel from 83 quality-screened subjects drawn from 113 recordings. Time-domain (TD) features were classified with multilayer perceptrons (MLPs) and three uncertainty quantification (UQ) methods: Monte Carlo (MC) Dropout, Deep Ensembles, and Evidential Deep Learning (EDL). A raw-window one-dimensional convolutional neural network (1D-CNN) and a Temporal Convolutional Network (TCN) were included as end-to-end comparators. The best within-subject result was obtained by Random Forest at [Formula: see text], while full-coverage leave-one-subject-out cross-validation (LOSOCV) accuracy was [Formula: see text] for the MLP baseline; all UQ methods were statistically indistinguishable from this baseline after Holm correction. Confidence-based selective prediction improved per-subject accuracy to [Formula: see text] at [Formula: see text] coverage and [Formula: see text] at [Formula: see text] coverage, but this gain was achieved equally by plain MLP softmax confidence and by MC Dropout, indicating that the improvement arises from the confidence-ordering property of softmax outputs rather than from stochastic inference. The deterministic MLP achieved the best calibration (Expected Calibration Error, ECE[Formula: see text]), while EDL showed vacuity collapse and was the only method to degrade under selective prediction (ECE[Formula: see text]). Both 1D-CNN and TCN showed numerically lower LOSOCV accuracy than the TD+MLP pipeline under the evaluated preprocessing configuration, though the differences were not statistically significant after Holm correction; loss of amplitude information through per-sample normalisation is a plausible contributing factor. Per-class analysis revealed that selective prediction gains are structurally concentrated in the rest class, which is retained at [Formula: see text] coverage with near-perfect accuracy ([Formula: see text]); excluding rest, active-gesture accuracy at [Formula: see text] coverage was only [Formula: see text]. This rest-class dominance effect has direct implications for how selective prediction should be evaluated in sEMG datasets that include a rest class. External validation on NinaPro DB2 (40 subjects, one circumferential forearm channel) confirmed above-chance classification ([Formula: see text]) but showed no meaningful correlation between uncertainty and per-subject accuracy ([Formula: see text]), indicating that the UQ mechanism does not generalise to this dataset. These results indicate that standard softmax confidence, requiring no additional training or inference overhead, is sufficient for rejection-based low-resource sEMG deployment under the evaluated conditions; computationally expensive stochastic inference methods provide no measurable additional benefit on low-dimensional tabular features. CI - (c) 2026. The Author(s). FAU - Aydogan, Yigit AU - Aydogan Y AUID- ORCID: 0009-0005-6263-4636 AD - Department of Computer Science, Aberystwyth University, Aberystwyth, UK. yia@aber.ac.uk. FAU - Canturk, Ismail AU - Canturk I AD - Department of Biomedical Engineering, Medical Intelligence Research Centre, Yildiz Technical University, Istanbul, Turkiye. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Med Biol Eng Comput JT - Medical & biological engineering & computing JID - 7704869 SB - IM OTO - NOTNLM OT - NinaPro OT - Selective prediction OT - Subject-independent evaluation OT - Surface electromyography OT - Temporal convolutional network OT - Uncertainty quantification OT - hand gesture recognition COIS- Declarations. Competing Interests: The authors declare that they have no competing financial or non-financial interests. Ethical Approval: This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Yildiz Technical University Ethics Committee under decision no. 2022.12. Consent to participate: Informed consent was obtained from all participants prior to data collection. Consent for publication: Not applicable. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:22 PHST- 2026/05/17 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:22 [entrez] AID - 10.1007/s11517-026-03704-y [pii] AID - 10.1007/s11517-026-03704-y [doi] PST - aheadofprint SO - Med Biol Eng Comput. 2026 Oct 28. doi: 10.1007/s11517-026-03704-y. PMID- 42946881 OWN - NLM STAT- Publisher LR - 20261028 IS - 1463-1326 (Electronic) IS - 1462-8902 (Linking) DP - 2026 Oct 27 TI - Assessment of Non-Invasive Tests for MASLD, MASH and At-Risk MASH in a Multicentre Prospective Real-World Study. LID - 10.1111/dom.71469 [doi] AB - BACKGROUND AND AIMS: Accurate non-invasive identification of metabolic dysfunction-associated steatohepatitis (MASH) and at-risk MASH remains a major unmet need in MASLD. We evaluated the diagnostic performance and optimal cutoffs of commonly used non-invasive tests (NITs) in a real-world setting. METHODS: In this multicentre prospective study, 328 cases from 226 participants underwent contemporaneous liver biopsy, MRI-PDFF, FibroScan and biochemical assessment, and were classified into MASL, MASH and at-risk MASH based on histopathology. Diagnostic performance was evaluated using area under the receiver operating characteristic curve (AUC), sensitivity and specificity. RESULTS: CAP at 248 dB/m detected histological steatosis with AUC of 0.80 (sensitivity: 94.7%, specificity: 30.4%); raising the threshold to 304 dB/m increased specificity to 91.3% (sensitivity: 60.3%). The two-step CAP-ALT algorithm achieved a more balanced sensitivity and specificity (78.3% and 82.6%, respectively; AUC: 0.80). MRI-PDFF quantified steatosis accurately (AUC: 0.92; cutoff: 7.78%; sensitivity: 77.1%; specificity: 100%) and differentiated grades, with diagnostic discordance versus histopathology mainly in mild disease. FAST score (AUC: 0.85; cutoff: 0.29; sensitivity: 70.0%; specificity: 83.3%) and MRI-PDFF (AUC: 0.82; cutoff: 17.78%; sensitivity: 56.2%; specificity: 92.6%) identified MASH, while LSM (AUC: 0.92; cutoff: 8.95 kPa; sensitivity: 86.5%; specificity: 84.1%) detected at-risk MASH. CONCLUSIONS: CAP provides an accessible approach for steatosis detection, whereas MRI-PDFF offers quantitative assessment of liver fat and may provide additional information relevant to MASH. FAST may serve as an initial tool for MASH risk stratification, while LSM provides effective identification of at-risk MASH. These findings support a targeted, stepwise non-invasive approach to MASLD assessment and follow-up. CI - (c) 2026 John Wiley & Sons Ltd. FAU - Xu, Jia AU - Xu J AD - Phase I Clinical Trial Centre, First Hospital of Jilin University, Changchun, China. FAU - Zhu, Mingyu AU - Zhu M AUID- ORCID: 0000-0003-1137-0915 AD - Department of Gastroenterology, Ruijin Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China. FAU - Zhu, Xiaoxue AU - Zhu X AD - Phase I Clinical Trial Centre, First Hospital of Jilin University, Changchun, China. FAU - Jia, Haiyan AU - Jia H AD - Phase I Clinical Trial Centre, First Hospital of Jilin University, Changchun, China. FAU - Zhang, Hong AU - Zhang H AD - Phase I Clinical Trial Centre, First Hospital of Jilin University, Changchun, China. FAU - Lu, Mengdi AU - Lu M AD - Phase I Clinical Trial Centre, First Hospital of Jilin University, Changchun, China. FAU - Chen, Li AU - Chen L AD - Department of Gastroenterology, Ruijin Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China. FAU - Ding, Yanhua AU - Ding Y AUID- ORCID: 0000-0003-2320-4404 AD - Phase I Clinical Trial Centre, First Hospital of Jilin University, Changchun, China. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Diabetes Obes Metab JT - Diabetes, obesity & metabolism JID - 100883645 SB - IM OTO - NOTNLM OT - MASH OT - at-risk MASH OT - cutoff OT - multicentre EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:22 PHST- 2026/09/27 00:00 [revised] PHST- 2026/07/26 00:00 [received] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:22 [entrez] AID - 10.1111/dom.71469 [doi] PST - aheadofprint SO - Diabetes Obes Metab. 2026 Oct 27. doi: 10.1111/dom.71469. PMID- 42946877 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1863-2491 (Electronic) IS - 1863-2483 (Linking) VI - 20 IP - 1 DP - 2026 Oct 28 TI - Robot-assisted versus conventional cranial stereotactic neurosurgery: a systematic review and meta-analysis of targeting accuracy, procedural efficiency, and safety. LID - 1147 [pii] LID - 10.1007/s11701-026-04111-z [doi] AB - Comparative evidence on robotic cranial stereotaxy spans different procedures, platforms and outcome definitions. We evaluated targeting accuracy, procedural efficiency, safety and clinical outcomes by surgical application. Five databases were searched through September 20, 2026. Twenty-one comparative studies were included. Accuracy was summarized using Hedges g and time using mean differences (conventional minus robotic). DerSimonian-Laird (DL)/Wald analyses were compared with alternative variance estimators and Hartung-Knapp (HK) intervals. Surgical applications and clinical outcomes were assessed separately. The exploratory three-study accuracy synthesis favored robotic assistance under DL/Wald (g=1.03, 95% CI 0.36-1.69; I(2)=78.5%), but its prediction interval crossed zero (- 0.189 to 2.242). REML/HK yielded g=1.03 (95% CI -0.41-2.47; P=0.092); modified HK gave a similar result. Inclusion of electrode-level SEEG also produced a confidence interval crossing zero. Two biopsy studies favored shorter procedure duration under DL/Wald (18.48 min, 95% CI 11.46-25.49), although HK intervals crossed zero. Total biopsy workflow time and intracerebral hemorrhage drainage duration were inconclusive. Risk-of-bias judgments were serious in 17 studies, critical in one, moderate in one, and some concerns in two. No consistent association between improved targeting and better clinical outcomes was established. Selected biopsy cohorts suggest technical accuracy and timing advantages with robotic assistance. Few studies, bias, heterogeneity and sensitivity to interval estimation prevent conclusions of generalized clinical superiority across cranial stereotactic applications. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature. FAU - Dong, Yiqun AU - Dong Y AD - Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, Guangdong, China. FAU - Zhang, Shuo AU - Zhang S AD - Department of Rehabilitation Therapy, School of Rehabilitation Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong Province, China. FAU - Bao, Yanxi AU - Bao Y AD - Nanjing University of Chinese Medicine, Nanjing, 210023, Jiangsu, China. FAU - Jiang, Yuxuan AU - Jiang Y AD - Southwest Medical University, Luzhou, 646000, China. FAU - Liu, Baoying AU - Liu B AD - Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, Guangdong, China. FAU - Li, Zerui AU - Li Z AD - Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, Guangdong, China. FAU - Wen, Zhuoxin AU - Wen Z AD - Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, Guangdong, China. FAU - Jia, Tianxia AU - Jia T AD - Department of Rehabilitation Therapy, School of Rehabilitation Medicine, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong Province, China. FAU - Li, Xifeng AU - Li X AD - Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, Guangdong, China. nflxf@126.com. FAU - Duan, Chuanzhi AU - Duan C AD - Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, Guangdong, China. doctor_duanzj@163.com. FAU - Liu, Yanchao AU - Liu Y AD - Department of Neurosurgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, Guangdong, China. liuyanchao_zj@126.com. LA - eng PT - Comparative Study PT - Journal Article PT - Meta-Analysis PT - Review PT - Systematic Review DEP - 20261028 PL - England TA - J Robot Surg JT - Journal of robotic surgery JID - 101300401 SB - IM MH - Humans MH - *Robotic Surgical Procedures/methods MH - *Stereotaxic Techniques MH - *Neurosurgical Procedures/methods MH - Operative Time MH - *Skull/surgery OTO - NOTNLM OT - Brain biopsy OT - Meta-analysis OT - Procedural efficiency OT - Stereotactic neurosurgery OT - Surgical robotics OT - Targeting accuracy COIS- Declarations. Competing interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. Consent for publication: Not applicable. Ethical approval and consent to participate: Not applicable. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 00:22 PHST- 2026/09/28 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:22 [entrez] AID - 10.1007/s11701-026-04111-z [pii] AID - 10.1007/s11701-026-04111-z [doi] PST - epublish SO - J Robot Surg. 2026 Oct 28;20(1):1147. doi: 10.1007/s11701-026-04111-z. PMID- 42946870 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 0942-0940 (Electronic) IS - 0001-6268 (Print) IS - 0001-6268 (Linking) VI - 168 IP - 1 DP - 2026 Jul 30 TI - Comparison of rebleeding risk after navigation-guided catheterization and thrombolytic irrigation using tPA or urokinase in intracerebral hemorrhage. LID - 10.1007/s00701-026-06995-0 [doi] LID - 250 AB - BACKGROUND: Minimally invasive catheter-based evacuation is increasingly used for spontaneous intracerebral hemorrhage (ICH). Urokinase and tissue plasminogen activator (tPA) are used for intracatheter thrombolytic irrigation; however, direct comparative data on their safety, particularly regarding rebleeding risk, remain limited. This study aimed to compare rebleeding risk and early outcomes between urokinase and tPA after navigation-guided catheterization. METHODS: We retrospectively analyzed adults with spontaneous supratentorial ICH who underwent stereotactic, navigation-guided catheter placement followed by scheduled intracavitary thrombolysis between January 2018 and December 2023. Patients received either urokinase or tPA. The primary outcome was rebleeding, defined as hematoma expansion or recurrence on imaging with neurological deterioration. Secondary outcomes were hematoma volume change from baseline to day 4 and short-term changes in modified Rankin Scale (mRS) and Glasgow Coma Scale (GCS). Penalized logistic regression, multivariable linear regression, and ordinal logistic regression models were used to adjust for baseline imbalances and evaluate radiologic and short-term functional outcomes. RESULTS: Eighty patients were included (urokinase, n = 35; tPA, n = 45). tPA-treated patients presented with higher initial systolic blood pressure and tended to have larger baseline hematoma volumes. Rebleeding occurred in 12/45 patients (26.7%) in the tPA group versus 2/35 (5.7%) in the urokinase group (p = 0.032), and the presence of concurrent intraventricular hemorrhage (IVH) was a potent risk factor. After adjustment for concurrent IVH, symptom onset-to-surgery time, and baseline hematoma volume using a penalized multivariable model, tPA use remained associated with rebleeding (adjusted odds ratio,5.03; 95% confidence interval,1.12-22.64; p = 0.035). Mean hematoma volume reduction by day 4 was similar between groups, although volume change was more variable in the tPA group. Early shifts in mRS and GCS favored urokinase. CONCLUSIONS: In catheter-based minimally invasive treatment of spontaneous supratentorial ICH, urokinase was associated with a lower risk of rebleeding and more favorable early neurological outcomes than tPA, despite comparable mean hematoma reduction. CI - (c) 2026. The Author(s). FAU - Song, Dong Hoon AU - Song DH AD - Department of Neurosurgery, Ewha Womans University Seoul Hospital, College of Medicine, Ewha Womans University, 260 Gonghang-daero, Gangseo-gu, Seoul, 07804, Republic of Korea. FAU - Cho, Dong Young AU - Cho DY AD - Department of Neurosurgery, Ewha Womans University Seoul Hospital, College of Medicine, Ewha Womans University, 260 Gonghang-daero, Gangseo-gu, Seoul, 07804, Republic of Korea. nsdyc0501@gmail.com. FAU - Koo, Ja Ho AU - Koo JH AD - Department of Neurosurgery, Ewha Womans University Seoul Hospital, College of Medicine, Ewha Womans University, 260 Gonghang-daero, Gangseo-gu, Seoul, 07804, Republic of Korea. FAU - Seo, Eu Kyo AU - Seo EK AD - Department of Neurosurgery, Ewha Womans University Seoul Hospital, College of Medicine, Ewha Womans University, 260 Gonghang-daero, Gangseo-gu, Seoul, 07804, Republic of Korea. AD - Department of Neurosurgery, Ewha Womans University Mokdong Hospital, College of Medicine, Ewha Womans University, 1071 Anyangcheon-ro, Yangcheon-gu, Seoul, 07985, Republic of Korea. LA - eng PT - Comparative Study PT - Journal Article DEP - 20260730 PL - Austria TA - Acta Neurochir (Wien) JT - Acta neurochirurgica JID - 0151000 RN - EC 3.4.21.68 (Tissue Plasminogen Activator) RN - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator) RN - 0 (Fibrinolytic Agents) SB - IM MH - Humans MH - *Tissue Plasminogen Activator/therapeutic use/administration & dosage MH - *Urokinase-Type Plasminogen Activator/therapeutic use/administration & dosage MH - *Cerebral Hemorrhage/drug therapy/therapy/surgery MH - Female MH - Male MH - *Thrombolytic Therapy/methods MH - Aged MH - Retrospective Studies MH - *Fibrinolytic Agents/therapeutic use/administration & dosage MH - Middle Aged MH - Recurrence MH - *Neuronavigation MH - Therapeutic Irrigation/methods MH - Catheterization/methods MH - Treatment Outcome PMC - PMC13714056 OTO - NOTNLM OT - Intracerebral hemorrhage OT - Minimally invasive surgery OT - Rebleeding OT - Thrombolysis OT - Tissue plasminogen activator OT - Urokinase COIS- Declarations. Ethics approval and consent to participate: This study was approved by the Institutional Review Boards of the participating institutions. The requirement for informed consent was waived because of the retrospective nature of the analysis. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/30 CRDT- 2026/10/28 00:22 PHST- 2026/04/07 00:00 [received] PHST- 2026/07/24 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:22 [entrez] PHST- 2026/07/30 00:00 [pmc-release] AID - 10.1007/s00701-026-06995-0 [pii] AID - 6995 [pii] AID - 10.1007/s00701-026-06995-0 [doi] PST - epublish SO - Acta Neurochir (Wien). 2026 Jul 30;168(1):250. doi: 10.1007/s00701-026-06995-0. PMID- 42946866 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1530-9932 (Electronic) IS - 1530-9932 (Linking) VI - 27 IP - 8 DP - 2026 Oct 27 TI - Overcoming Biopharmaceutical Challenges in the Development of Amorphous Nanosuspension: A Case Study of Atazanavir. LID - 342 [pii] LID - 10.1208/s12249-026-03574-5 [doi] AB - Amorphous nanosuspensions can generate supersaturated drug concentrations far above crystalline solubility, but their performance depends on stabilizing both the metastable amorphous phase and the high interfacial energy of nanosized particles against recrystallization and Ostwald ripening. ATZ was selected as a model weakly basic drug because its poor dissolution and rapid desupersaturation at intestinal pH provide a rational formulation space for probing the biopharmaceutical relevance of amorphous nanosuspensions. The present study addressed these limitations through the development of ATZ amorphous nanosuspension using acid-base neutralization method. Polymer-surfactant screening was performed through desupersaturation assay and drug-rich droplet size analysis which identified HPMCAS-MG and Soluplus as optimal precipitation inhibitors. Box-Behnken design optimization yielded nanosuspension with a mean particle size of 146.7 +/- 5.56 nm and polydispersity index of 0.261 +/- 0.05, and a zeta potential of - 4.54 +/- 0.78 mV. Solid-state and spectroscopic analyses confirmed successful amorphization of ATZ and the presence of hydrogen-bonding and hydrophobic interactions in ATZ-ANS. Further, non-sink in vitro dissolution testing revealed that ATZ-ANS effectively sustained supersaturation with limited precipitation. The formulation retained its nanoscale characteristics and did not show evidence of recrystallization during six months of accelerated stability testing at 40 degrees C/75% relative humidity (RH). The in vivo pharmacokinetic evaluation in Sprague Dawley rats demonstrated that ATZ-ANS achieved 16.59-fold higher C(max) (ng/mL) and 6.31-fold higher AUC(0-t) (ng-h/mL) than crystalline ATZ, and a 2.08-fold and 3.22-fold increase over the marketed formulation (Reyataz(R)), respectively. Our findings highlighted amorphous nanosuspension as a pragmatic approach for difficult to deliver drugs. CI - (c) 2026. The Author(s), under exclusive licence to American Association of Pharmaceutical Scientists. FAU - Saini, Vanshul AU - Saini V AUID- ORCID: 0009-0009-9322-732X AD - Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, S.A.S. Nagar, Punjab, 160062, India. FAU - Sangamwar, Abhay T AU - Sangamwar AT AUID- ORCID: 0000-0002-0259-9956 AD - Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, S.A.S. Nagar, Punjab, 160062, India. abhays@niper.ac.in. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - AAPS PharmSciTech JT - AAPS PharmSciTech JID - 100960111 RN - 0 (Suspensions) RN - 4MT4VIE29P (Atazanavir Sulfate) RN - 0 (Surface-Active Agents) RN - 0 (Bulk Drugs) RN - 0 (Polyvinyls) RN - 0 (Polymers) RN - 3WJQ0SDW1A (Polyethylene Glycols) RN - 0 (polyvinyl caprolactam-polyvinyl acetate-polyethylene glycol graft copolymer) SB - IM MH - *Nanoparticles/chemistry MH - Solubility MH - Particle Size MH - Suspensions/chemistry MH - *Atazanavir Sulfate/chemistry/pharmacokinetics MH - Animals MH - Chemistry, Pharmaceutical/methods MH - Crystallization/methods MH - Surface-Active Agents/chemistry MH - Bulk Drugs MH - Polyvinyls/chemistry MH - Polymers/chemistry MH - Polyethylene Glycols/chemistry MH - Rats MH - Drug Compounding/methods MH - Hydrophobic and Hydrophilic Interactions MH - Drug Stability OTO - NOTNLM OT - amorphous nanosuspension OT - atazanavir OT - dissolution OT - pharmacokinetics OT - precipitation OT - supersaturation COIS- Declarations. Ethics Approval: All in vivo studies were conducted in accordance with internationally accepted standards for the ethical use and care of laboratory animals. Experimental procedures followed the ARRIVE reporting framework and complied with applicable animal welfare regulations, including the U.K. Animals (Scientific Procedures) Act, 1986, and EU Directive 2010/63/EU. The study protocol (IAEC/23/70) received prior approval from the Institutional Animal Ethics Committee of NIPER, S.A.S. Nagar, India. Competing interests: The authors declare that there are no known financial interests, commercial associations, or personal relationships that could have influenced the outcomes or interpretation of the work reported in this manuscript. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 00:22 PHST- 2026/06/03 00:00 [received] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:22 [entrez] AID - 10.1208/s12249-026-03574-5 [pii] AID - 10.1208/s12249-026-03574-5 [doi] PST - epublish SO - AAPS PharmSciTech. 2026 Oct 27;27(8):342. doi: 10.1208/s12249-026-03574-5. PMID- 42946865 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 1420-9071 (Electronic) IS - 1420-682X (Print) IS - 1420-682X (Linking) VI - 83 IP - 1 DP - 2026 Jul 31 TI - SELENOP-LRP8-mediated macrophage-endothelial cell interaction promotes breast cancer vascular invasion by inducing metabolic reprogramming in endothelial cells. LID - 10.1007/s00018-026-06359-y [doi] LID - 399 AB - BACKGROUND: Vascular invasion (VI) is an early event in breast cancer dissemination. However, the mechanisms by which macrophages and endothelial cells cooperate to drive this process remain incompletely understood. We aimed to identify macrophage subsets associated with VI and determine how they influence endothelial cell behavior. METHODS: Single-cell RNA sequencing and spatial transcriptomic profiling and were conducted on breast cancer specimens partitioned by their vascular invasion characteristics. Multiplex immunofluorescence, coculture assays, transcriptomic, proteomic, and metabolomic profiling, molecular docking, and co-immunoprecipitation were used to characterize the SELENOP-LRP8 axis. In vivo validation was conducted using a myeloid-specific Selenop knockout model (Selenop(f/f); Lyz2(Cre)) bearing E0771 allografts and treated with bevacizumab and/or Ebronucimab. RESULTS: A distinct SELENOP(+) macrophage subset was enriched in VI-positive breast tumors and exhibited close spatial proximity to endothelial cells. In vitro, macrophage-derived SELENOP enhanced endothelial migration and tube formation. Multi-omics analyses suggested that SELENOP binds endothelial LRP8, leading to cholesterol metabolic rewiring and Hedgehog pathway activation. In the mouse model, Selenop deficiency reduced tumor growth and vascularization, while combination treatment with bevacizumab and Ebronucimab produced the strongest inhibitory effect among the treatment groups. CONCLUSIONS: These findings identify a SELENOP(+) macrophage-endothelial metabolic axis associated with breast cancer VI and support further investigation of combined anti-angiogenic and cholesterol-pathway-directed strategies in preclinical models. CI - (c) 2026. The Author(s). FAU - Wang, Haoran AU - Wang H AD - Department of tumor surgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, 150081, China. FAU - Song, Yuan AU - Song Y AD - Department of tumor surgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, 150081, China. FAU - Ran, Mengdi AU - Ran M AD - Department of tumor surgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, 150081, China. FAU - Zhang, Rui AU - Zhang R AD - Department of tumor surgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, 150081, China. FAU - Sun, Weibo AU - Sun W AD - Department of tumor surgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, 150081, China. FAU - Giorgi, Oganezov AU - Giorgi O AD - Department of tumor surgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, 150081, China. FAU - Zhang, Wenxuan AU - Zhang W AD - Department of tumor surgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, 150081, China. FAU - Liu, Tong AU - Liu T AD - Department of tumor surgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, 150081, China. liutong@ems.hrbmu.edu.cn. FAU - Tai, Sheng AU - Tai S AD - Department of tumor surgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, 150081, China. taisheng1973@163.com. FAU - Qian, Cheng AU - Qian C AUID- ORCID: 0000-0001-8052-1006 AD - Department of tumor surgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, 150081, China. qiancheng@ems.hrbmu.edu.cn. LA - eng GR - No. 82173184/the National Natural Science Foundation of China/ GR - 2024ZD0520400 and 2024ZD0520406/the National Natural Science Foundation of China/ PT - Journal Article DEP - 20260731 PL - Switzerland TA - Cell Mol Life Sci JT - Cellular and molecular life sciences : CMLS JID - 9705402 SB - IM MH - Female MH - Animals MH - Humans MH - *Macrophages/metabolism/pathology MH - *Breast Neoplasms/pathology/metabolism/genetics/blood supply MH - *Endothelial Cells/metabolism/pathology MH - Metabolic Reprogramming MH - Mice MH - Cell Communication MH - Neovascularization, Pathologic/metabolism/pathology MH - Cell Line, Tumor MH - Neoplasm Invasiveness MH - Cell Movement MH - Mice, Knockout PMC - PMC13713971 OTO - NOTNLM OT - Breast cancer OT - Cholesterol metabolism OT - Hedgehog signaling OT - Macrophage-endothelial crosstalk OT - SELENOP OT - Vascular invasion COIS- Declarations. Ethics approval and consent to participate: Human tissue collection and animal experiments were conducted in accordance with approved ethical procedures. Written informed consent was obtained from patients before sample collection. The study was approved by the Ethics Committee of Harbin Medical University Cancer Hospital (No. KY202505). Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:21 PHST- 2026/05/14 00:00 [received] PHST- 2026/07/16 00:00 [accepted] PHST- 2026/06/30 00:00 [revised] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:21 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1007/s00018-026-06359-y [pii] AID - 6359 [pii] AID - 10.1007/s00018-026-06359-y [doi] PST - epublish SO - Cell Mol Life Sci. 2026 Jul 31;83(1):399. doi: 10.1007/s00018-026-06359-y. PMID- 42946850 OWN - NLM STAT- Publisher LR - 20261028 IS - 1432-1912 (Electronic) IS - 0028-1298 (Linking) DP - 2026 Oct 28 TI - Synergistic induction of apoptosis in human prostate cancer PC3 cells by silver-coated cerium oxide nanoparticles: unveiling the role of ROS-mediated p53-associated apoptotic signaling and antioxidant system depletion. LID - 10.1007/s00210-026-06002-4 [doi] AB - Castration-resistant prostate cancer (CRPC) represents a formidable clinical challenge due to intrinsic defects in apoptotic execution, elevated baseline antioxidant capacity, and rapid resistance to standard chemotherapy. Herein, we report the rational design, template-directed synthesis, and antineoplastic evaluation of silver-coated cerium oxide core-shell nanoparticles (Ag@CeO2 NPs) derived from a cerium-based metal-organic framework (Ce-UiO-66) for targeted intervention in androgen-independent prostate carcinoma. Uniform Ce-UiO-66 MOF nanoparticles were thermally converted via controlled calcination (550 degrees C, 3 h) into porous cubic fluorite nanoceria (CeO(2)) cores, followed by in situ surface nucleation and reduction of a metallic silver (Ag(0)) nanoshell stabilized by polyvinylpyrrolidone. The physicochemical properties were characterized by FTIR, PXRD, FESEM, EDX mapping, DLS, and Zeta potential analysis. In vitro anticancer efficacy was evaluated in human androgen-independent PC-3 prostate cancer cells and normal human fibroblasts (HFF-1). Cellular viability, intracellular reactive oxygen species (ROS) production, superoxide dismutase (SOD) and catalase (CAT) enzymatic kinetics, apoptosis-related transcriptomics (p53, Bax, Bcl-2 via qRT-PCR), and p53 protein levels (ELISA) were systematically quantified. PXRD and Scherrer analysis confirmed the formation of pure, face-centered cubic fluorite CeO(2) (Cerianite-(Ce), Fm3̄m, *a* = 5.4110 A) with an average primary crystallite size of 7.27 +/- 0.36 nm (7.44 nm for the (111) reflection), alongside characteristic reflections of metallic Ag. FESEM, DLS, and electrophoretic measurements revealed spherical, cauliflower-like hierarchical clusters with a hydrodynamic diameter (Z-average) of 111.68 nm, narrow polydispersity (PDI = 0.2860), and stable negative surface charge (zeta = - 14.96 mV). The Ag@CeO2 NPs displayed pronounced, tumor-selective cytotoxicity, yielding an IC(5)(0) of 32.14 microg/mL against PC-3 cells while sparing normal HFF-1 fibroblasts (IC(5)(0) = 171.6 microg/mL, > 5.3-fold selectivity window). Mechanistically, the nanocomposite triggered a severe, concentration-dependent intracellular ROS burst (*p* < 0.0001 at 30 microM) accompanied by the functional collapse of the cellular antioxidant barrier through profound suppression of SOD (*p* < 0.01) and CAT (*p* < 0.0001) activities. This unmitigated oxidative crisis was associated with increased TP53 transcript levels and increased p53-associated protein signals as measured by ELISA (*p* < 0.0001), which coincided with upregulated Bax, downregulated Bcl-2, and elevated the Bax/Bcl-2 ratio (*p* < 0.001), consistent with activation of mitochondrial apoptotic signaling. Ag@CeO2 NPs function as a potent, tumor-selective pro-oxidant nanotherapeutic platform capable of overriding endogenous antioxidant defenses, enhancing p53-associated apoptotic signaling and promoting mitochondrial apoptotic responses, suggesting that Ag@CeO2 NPs may serve as a potential candidate for further investigation in prostate cancer nanomedicine. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Al-Awassi, Hiam AU - Al-Awassi H AUID- ORCID: 0009-0003-6451-2857 AD - Department of Basic Medical Sciences, Inaya Medical Colleges, Riyadh, Saudi Arabia. HiamAlawassi@protonmail.com. FAU - AlAbdulhadi, Saleh A S AU - AlAbdulhadi SAS AD - Department of Medical Laboratory Science, Prince Sattam Bin Abdulaziz University, Riyadh, Saudi Arabia. FAU - Aslam, Akhmed AU - Aslam A AD - Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah, Saudi Arabia. FAU - AlMoshary, May AU - AlMoshary M AD - Department of Basic Science, College of Medicine, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, 11671, Riyadh, Saudi Arabia. FAU - Alrefaei, Ghadeer Ibrahem AU - Alrefaei GI AD - Department of Biological Sciences, College of Science, University of Jeddah, Jeddah, Saudi Arabia. FAU - Al-Qahtani, Saeed A AU - Al-Qahtani SA AD - Department of Pharmacy Practice, College of Pharmacy, Jazan University, 45142, Jazan, Saudi Arabia. FAU - Barnawi, Jameel AU - Barnawi J AD - Department of Medical Laboratory Technology, University of Tabuk, Tabuk, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261028 PL - Germany TA - Naunyn Schmiedebergs Arch Pharmacol JT - Naunyn-Schmiedeberg's archives of pharmacology JID - 0326264 SB - IM OTO - NOTNLM OT - Bax/Bcl-2 apoptotic rheostat OT - Castration-resistant prostate cancer (PC-3) OT - Ce-UiO-66 metal-organic framework OT - P53-associated apoptotic signaling OT - Reactive oxygen species (ROS) OT - Silver-coated cerium oxide (CeO(2)@Ag) OT - Superoxide dismutase and catalase depletion OT - Targeted cancer nanomedicine COIS- Declarations. Ethics approval: Ethical approval is not applicable. Conflict of interest: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:21 PHST- 2026/08/16 00:00 [received] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:21 [entrez] AID - 10.1007/s00210-026-06002-4 [pii] AID - 10.1007/s00210-026-06002-4 [doi] PST - aheadofprint SO - Naunyn Schmiedebergs Arch Pharmacol. 2026 Oct 28. doi: 10.1007/s00210-026-06002-4. PMID- 42946848 OWN - NLM STAT- Publisher LR - 20261028 IS - 1432-1912 (Electronic) IS - 0028-1298 (Linking) DP - 2026 Oct 28 TI - Contraindication-adjusted prescription omission patterns of RASi, beta-blockers, and MRAs in hospitalized heart failure: associations with 30-day and 1-year mortality. LID - 10.1007/s00210-026-06024-y [doi] AB - Guideline directed therapy is still underused in heart failure and little is known about how missing specific medications near discharge relates to survival. The aim of this study is to examine whether study-defined omission patterns of RASi (ACE inhibitors, ARBs, or ARNIs), beta-blockers, and mineralocorticoid receptor antagonists (MRAs) in the final 48 h before discharge were associated with short-term and 1-year mortality among hospitalized adults with heart failure. Adult admissions with a recorded heart failure diagnosis were identified using ICD-9 and ICD-10 codes. The three classes were RASi, beta-blockers, and MRAs (SGLT2 inhibitors were not included). A contraindication-adjusted operational framework was used to identify admissions without selected laboratory or hemodynamic barriers to these therapies and to classify late-hospital prescription omission patterns. Final-48-h orders were a proxy for treatment near discharge, not a reconciled discharge list. This framework did not establish heart failure phenotype-specific guideline eligibility. Associations with mortality within 30 and 365 days after discharge were evaluated using multivariable regression models. The final analytic cohort included 57,098 admissions from 24,922 patients. The reference group with prescriptions for all three study classes was small whereas omission of 2 or 3 therapies was substantially more common across the cohort. Admissions with prescriptions for all three study classes had the lowest 365-day mortality (13.88%). Crude 365-day mortality was higher in all omission groups, reaching 30.84% when RASi and beta-blocker were omitted and 27.50% when all three classes were omitted. In adjusted analyses, omission of all 3 therapies was associated with higher 30-day mortality (OR = 1.67). For 365-day mortality, the strongest adverse association was observed for omission of renin-angiotensin system inhibitor plus beta-blocker (HR = 1.33). Late-hospital omission patterns involving RASi and omission of all three study therapies were associated with higher post-discharge mortality. As heart failure phenotype and left-ventricular ejection fraction were not incorporated into the eligibility definition, these findings should be interpreted as prognostic associations of prescribing patterns rather than causal effects of withholding guideline-indicated HFrEF therapy. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Ishaqui, Azfar Athar AU - Ishaqui AA AD - Department of Clinical Pharmacy, College of Pharmacy, King Khalid University, Abha, Saudi Arabia. azfar.hd@hotmail.com. FAU - Tripathi, Rina AU - Tripathi R AD - Department of Pharmacy Practice, College of Clinical Pharmacy, King Faisal University, Al Ahsa, Saudi Arabia. FAU - Tripathi, Pankaj AU - Tripathi P AD - Department of Nursing, Almoosa College of Health Science, Al Ahsa, Saudi Arabia. FAU - Makeen, Hafiz AU - Makeen H AD - Department of Pharmacy Practice, Pharmacy Practice Research Unit, College of Pharmacy, Jazan University, Jazan, Saudi Arabia. FAU - Khardali, Amani AU - Khardali A AD - Department of Pharmacy Practice, Pharmacy Practice Research Unit, College of Pharmacy, Jazan University, Jazan, Saudi Arabia. FAU - Orayj, Khalid AU - Orayj K AD - Department of Clinical Pharmacy, College of Pharmacy, King Khalid University, Abha, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261028 PL - Germany TA - Naunyn Schmiedebergs Arch Pharmacol JT - Naunyn-Schmiedeberg's archives of pharmacology JID - 0326264 SB - IM OTO - NOTNLM OT - Beta-blockers OT - Heart failure OT - Mineralocorticoid receptor antagonists OT - Mortality OT - Renin-angiotensin system inhibitors OT - Therapy omission patterns COIS- Declarations. Ethics approval and consent to participate: This retrospective study used deidentified data from MIMIC-IV version 3.1, available through PhysioNet under credentialed access. The database contains deidentified hospital data and granted a waiver of informed consent The authors completed the required human-subjects protection training and complied with the PhysioNet Credentialed Health Data Use Agreement. Consent for publication: All authors have agreed to the publication of this study. Clinical trial number: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:21 PHST- 2026/08/05 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:21 [entrez] AID - 10.1007/s00210-026-06024-y [pii] AID - 10.1007/s00210-026-06024-y [doi] PST - aheadofprint SO - Naunyn Schmiedebergs Arch Pharmacol. 2026 Oct 28. doi: 10.1007/s00210-026-06024-y. PMID- 42946847 OWN - NLM STAT- Publisher LR - 20261028 IS - 1432-1912 (Electronic) IS - 0028-1298 (Linking) DP - 2026 Oct 28 TI - Combined melatonin and edaravone therapy attenuates methylmercury-induced amyotrophic lateral sclerosis-like neurodegeneration through GLP-1 modulation and myelin preservation. LID - 10.1007/s00210-026-06009-x [doi] AB - Amyotrophic lateral sclerosis (ALS) is a progressive and fatal neurodegenerative disorder characterized by the selective degeneration of upper and lower motor neurons, resulting in progressive muscle weakness, paralysis, and respiratory failure. Despite the availability of disease-modifying therapies, current treatment options provide only modest clinical benefits, highlighting the need for novel therapeutic strategies targeting multiple pathological mechanisms. Melatonin, a pleiotropic neuroprotective molecule with antioxidant, anti-inflammatory, and anti-apoptotic properties, has emerged as a promising candidate; however, its influence on glucagon-like peptide-1 (GLP-1)-associated neuroprotective signaling and related molecular events in ALS remains poorly understood. Therefore, the present study investigated the neuroprotective efficacy of melatonin in a methylmercury (MTMR)-induced rat model of ALS and explored its effects on GLP-1-associated molecular alterations, neuroinflammation, oxidative stress, apoptosis, neurotransmitter homeostasis, demyelination, and neuronal injury. Molecular docking analysis was performed to predict the interaction of melatonin with proteins implicated in ALS pathology. Adult Wistar rats were exposed to MTMR (5 mg/kg, p.o.) for 21 days to induce ALS-like neurodegeneration, followed by treatment with melatonin (5 or 10 mg/kg, i.p.), edaravone (3 mg/kg, i.p.), or their combination for an additional 21 days. Neurobehavioral performance, biochemical alterations, hematological parameters, molecular biomarkers, and histopathological changes were subsequently evaluated. MTMR administration produced profound motor deficits accompanied by reduced GLP-1 levels, neurotransmitter imbalance, increased neuroinflammatory and apoptotic markers, elevated neurofilament light chain (NEFL), skeletal muscle injury, methylmercury accumulation, and extensive myelin and neuronal damage. Melatonin treatment significantly attenuated these pathological alterations in a dose-dependent manner by increasing GLP-1 levels, improving antioxidant status, reducing inflammatory and apoptotic responses, restoring neurotransmitter balance, preserving myelin integrity, and improving neuronal morphology across multiple brain regions and the spinal cord. Molecular docking further predicted favorable interactions between melatonin and several proteins associated with ALS pathophysiology, supporting its potential as a multitarget therapeutic agent. In most outcome measures, combined treatment with melatonin and edaravone produced greater neuroprotective effects than either intervention alone. Collectively, these findings demonstrate that melatonin substantially attenuates methylmercury-induced ALS-like neuropathology through coordinated modulation of multiple pathological processes associated with neurodegeneration. Although the precise molecular mechanisms require further experimental validation, the observed restoration of GLP-1 levels together with improvements in oxidative stress, neuroinflammation, apoptosis, neurotransmitter homeostasis, and myelin integrity suggests that melatonin may represent a promising adjunctive therapeutic strategy for ALS. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Raina, Gurudev Singh AU - Raina GS AD - Division of Neuroscience, Department of Pharmacology, ISF College of Pharmacy, Moga, Punjab, 142001, India. AD - IK Gujral Punjab Technical University, Jalandhar, Punjab, 144603, India. FAU - Mehan, Sidharth AU - Mehan S AUID- ORCID: 0000-0003-0034-835X AD - Division of Neuroscience, Department of Pharmacology, ISF College of Pharmacy, Moga, Punjab, 142001, India. sidh.mehan@gmail.com. FAU - Das Gupta, Ghanshyam AU - Das Gupta G AD - Department of Pharmaceutics, ISF College of Pharmacy, Moga, Punjab, India. LA - eng PT - Journal Article DEP - 20261028 PL - Germany TA - Naunyn Schmiedebergs Arch Pharmacol JT - Naunyn-Schmiedeberg's archives of pharmacology JID - 0326264 SB - IM OTO - NOTNLM OT - Amyotrophic lateral sclerosis OT - Demyelination OT - Edaravone OT - GLP-1 OT - Melatonin OT - Methylmercury OT - Neuroinflammation OT - Neuroprotection OT - Oxidative stress COIS- Declarations. Ethical approval: The study was carried out in accordance with the Institutional Animal Ethics Committee (IAEC) norms, with registration number 816/PO/ReBiBt/S/04/CPCSEA 06/08/2004 as ISFCP/IAEC/CCSEA/Meeting No: 04/2023/Procedure No. 21/Nov 04, 2023, approved by the RAB Committee, ISFCP, Moga, Punjab, India. Consent to publish: The authors approve of submitting the publication. Consent to participate: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:21 PHST- 2026/07/02 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:21 [entrez] AID - 10.1007/s00210-026-06009-x [pii] AID - 10.1007/s00210-026-06009-x [doi] PST - aheadofprint SO - Naunyn Schmiedebergs Arch Pharmacol. 2026 Oct 28. doi: 10.1007/s00210-026-06009-x. PMID- 42946843 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1530-9932 (Electronic) IS - 1530-9932 (Linking) VI - 27 IP - 8 DP - 2026 Oct 27 TI - Next-Generation Bioinspired Microneedle Systems for Peptide Delivery: Bridging Design and Translational Medicine. LID - 337 [pii] LID - 10.1208/s12249-026-03571-8 [doi] AB - Peptide-based therapies have transformed the management of chronic illnesses such as diabetes, cancer, and cardiovascular problems, owing to their remarkable specificity, effectiveness, and low toxicity. Nonetheless, their therapeutic efficacy is frequently impeded by obstacles such as inadequate bioavailability, fast enzymatic breakdown, and restricted systemic stability. Conventional delivery strategies, such as oral and intravenous injection, have considerable constraints in efficiently transporting peptides to target locations. Transdermal drug delivery systems, especially microneedles (MNs) and bioinspired microneedles (BMNs), have garnered considerable interest as a less invasive and effective option to surmount these obstacles. MNs form microchannels in the epidermis and dermis, facilitating the direct administration of peptides into systemic circulation, hence dramatically improving peptide delivery across the skin. Recent improvements in BMNs technology have enhanced peptide delivery through better mechanical strength, skin contact, and controlled release. This paper mainly focuses on the importance of peptide drugs, the obstacles related to their administration, and the potential of BMNs for effective transdermal delivery. We emphasize current advancements in BMNs that improve peptide stability, permeability, and therapeutic effectiveness, facilitating their practical use in the treatment of chronic and acute disorders. CI - (c) 2026. The Author(s), under exclusive licence to American Association of Pharmaceutical Scientists. FAU - Chakma, Marjita AU - Chakma M AUID- ORCID: 0000-0001-6769-7824 AD - Department of Pharmaceutics, College of Pharmacy, JSS University, Noida, Uttar Pradesh, India. FAU - Babu, R Jayachandra AU - Babu RJ AUID- ORCID: 0000-0003-0337-2291 AD - Department of Drug Discovery and Development, Harrison College of Pharmacy, Auburn University, Auburn, AL, 36849, USA. FAU - De, Anindita AU - De A AUID- ORCID: 0000-0002-0838-9474 AD - Department of Pharmaceutics, College of Pharmacy, JSS University, Noida, Uttar Pradesh, India. aninditanirupa@gmail.com. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - United States TA - AAPS PharmSciTech JT - AAPS PharmSciTech JID - 100960111 RN - 0 (Peptides) SB - IM MH - Microneedle Drug Delivery/methods MH - Humans MH - *Peptides/administration & dosage/chemistry MH - Administration, Cutaneous MH - Animals MH - *Drug Delivery Systems/methods MH - Translational Research, Biomedical/methods MH - Skin/metabolism MH - Skin Absorption MH - Needles OTO - NOTNLM OT - Bioinspired microneedle OT - Biomimetic OT - Microneedle OT - Peptides OT - Transdermal COIS- Declarations. Ethics Approval and Consent to Participate: Not applicable. This article is a review and does not involve any human or animal participants. Consent for Publication: Not applicable. Competing interests: The authors whose names are listed agree to the submission and certify that they have no scientific or financial conflicts of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 00:21 PHST- 2026/05/26 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:21 [entrez] AID - 10.1208/s12249-026-03571-8 [pii] AID - 10.1208/s12249-026-03571-8 [doi] PST - epublish SO - AAPS PharmSciTech. 2026 Oct 27;27(8):337. doi: 10.1208/s12249-026-03571-8. PMID- 42946816 OWN - NLM STAT- Publisher LR - 20261028 IS - 1750-2799 (Electronic) IS - 1750-2799 (Linking) DP - 2026 Oct 27 TI - In situ assembly of peptides in living cells. LID - 10.1038/s41596-026-01433-y [doi] AB - The self-assembly of peptides into well-defined nanostructures represents a versatile strategy for developing biomedical agents. However, cellular internalization of pre-assembled peptide nanostructures is often hampered by insufficient uptake and low selectivity toward target cells, leading to off-target concerns and limited therapeutic efficacy. The past few years have witnessed the development of in situ assembly in living cells as a new endeavor for optimizing the performance of peptide assemblies. In situ assembly of peptides refers to intracellular assembling processes regulated by biocompatible stimuli, thereby enhancing peptide internalization and locally forming assemblies at target sites. Recently, we have developed strategies for establishing in situ assembly of peptides in living cells dictated by stimuli-responsive unnatural amino acids. Here we detail the procedure for developing in situ peptide assembly systems in living cells through incorporating stimuli-responsive unnatural amino acids. We present a thorough workflow encompassing peptide design and synthesis as well as the characterization of peptide responsiveness to glutathione or sulfatase. Self-assembly conditions are described for experiments in solution, in murine macrophage cells, human cervical carcinoma cells and murine breast cancer cells. The applications of in situ-formed peptide assemblies for subcellular targeting and cancer therapy in murine breast tumor-bearing BALB/c mice are also presented. The described methodology provides a robust platform for developing in situ-formed peptide assemblies and advancing the clinical translation of peptide-based supramolecular therapeutics. Performance of this Protocol requires expertise in peptide chemistry, materials engineering and biomedical engineering, taking an expected 92-115 d to complete. CI - (c) 2026. Springer Nature Limited. FAU - Zhang, Peng AU - Zhang P AD - Key Laboratory of Functional Polymer Materials, Ministry of Education, State Key Laboratory of Medicinal Chemical Biology, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin, China. AD - The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha, China. FAU - Liu, Xin AU - Liu X AD - Key Laboratory of Functional Polymer Materials, Ministry of Education, State Key Laboratory of Medicinal Chemical Biology, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin, China. FAU - Wang, Hao AU - Wang H AD - Key Laboratory of Functional Polymer Materials, Ministry of Education, State Key Laboratory of Medicinal Chemical Biology, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin, China. FAU - Chen, Ninglin AU - Chen N AD - Key Laboratory of Functional Polymer Materials, Ministry of Education, State Key Laboratory of Medicinal Chemical Biology, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin, China. AD - The National and Local Joint Engineering Laboratory of Animal Peptide Drug Development, College of Life Sciences, Hunan Normal University, Changsha, China. FAU - Shi, Linqi AU - Shi L AUID- ORCID: 0000-0002-9534-795X AD - Key Laboratory of Functional Polymer Materials, Ministry of Education, State Key Laboratory of Medicinal Chemical Biology, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin, China. shilinqi@nankai.edu.cn. FAU - Yu, Zhilin AU - Yu Z AUID- ORCID: 0000-0002-7116-3304 AD - Key Laboratory of Functional Polymer Materials, Ministry of Education, State Key Laboratory of Medicinal Chemical Biology, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin, China. yzh026@nankai.edu.cn. LA - eng GR - 52273130/National Natural Science Foundation of China (National Science Foundation of China)/ PT - Journal Article PT - Review DEP - 20261027 PL - England TA - Nat Protoc JT - Nature protocols JID - 101284307 SB - IM COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:20 PHST- 2025/06/25 00:00 [received] PHST- 2026/07/13 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:20 [entrez] AID - 10.1038/s41596-026-01433-y [pii] AID - 10.1038/s41596-026-01433-y [doi] PST - aheadofprint SO - Nat Protoc. 2026 Oct 27. doi: 10.1038/s41596-026-01433-y. PMID- 42946812 OWN - NLM STAT- Publisher LR - 20261028 IS - 2397-334X (Electronic) IS - 2397-334X (Linking) DP - 2026 Oct 27 TI - Maternal inheritance accelerates sex chromosome degeneration and mitonuclear coevolution in a brood parasitic bird. LID - 10.1038/s41559-026-03216-w [doi] AB - The coevolution of interacting mitochondrial and nuclear genes is increasingly recognized as a critical component of organismal fitness and a potential source of genetic incompatibilities that promote speciation. In species with 'ZW' sex chromosomes, however, selection on maternally inherited traits may impact mitonuclear coevolution owing to the effective linkage of the W chromosome and mitochondrial DNA (mtDNA). Here we show that in brood parasitic cuckoo finches (Anomalospiza imberbis), enhanced ecological selection on a maternally inherited trait (mimetic egg colouration) subjects both the W chromosome and mtDNA to increased accumulation of slightly deleterious mutations via genetic hitchhiking, accelerating W chromosome pseudogenization and gene loss, as well as mitonuclear coevolution. Remarkably, as other W-linked genes were lost, two egg colour genes, representing likely targets of selection during cuckoo finch-host coevolution, were recruited to the W chromosome. Using structural models of the respiratory complexes and mitoribosome, we also show that the rate of amino acid substitution in nuclear-encoded residues is a function of their proximity to mtDNA-encoded residues, providing evidence of compensatory mitonuclear coevolution. Our results link species-level coevolution to both sex chromosome degeneration and mitonuclear coevolution. More generally, they highlight the genomic consequences of adaptation without recombination. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature Limited. FAU - Sorenson, Michael D AU - Sorenson MD AUID- ORCID: 0000-0001-5375-2917 AD - Department of Biology, Boston University, Boston, MA, USA. msoren@bu.edu. FAU - Osipova, Ekaterina AU - Osipova E AUID- ORCID: 0000-0002-6769-7223 AD - Informatics Group, Harvard University, Cambridge, MA, USA. FAU - Spottiswoode, Claire N AU - Spottiswoode CN AUID- ORCID: 0000-0003-3232-9559 AD - FitzPatrick Institute of African Ornithology, Department of Biological Sciences, University of Cape Town, Cape Town, South Africa. AD - Department of Zoology, University of Cambridge, Cambridge, UK. FAU - Jamie, Gabriel A AU - Jamie GA AD - FitzPatrick Institute of African Ornithology, Department of Biological Sciences, University of Cape Town, Cape Town, South Africa. AD - Department of Zoology, University of Cambridge, Cambridge, UK. FAU - Dixit, Tanmay AU - Dixit T AUID- ORCID: 0000-0001-5604-7965 AD - FitzPatrick Institute of African Ornithology, Department of Biological Sciences, University of Cape Town, Cape Town, South Africa. AD - Department of Zoology, University of Cambridge, Cambridge, UK. FAU - DaCosta, Jeffrey M AU - DaCosta JM AUID- ORCID: 0000-0002-8590-2083 AD - Biology Department, Boston College, Chestnut Hill, MA, USA. FAU - Sackton, Timothy B AU - Sackton TB AUID- ORCID: 0000-0003-1673-9216 AD - Informatics Group, Harvard University, Cambridge, MA, USA. FAU - Warren, Wesley C AU - Warren WC AUID- ORCID: 0000-0003-2255-2730 AD - Division of Animal Sciences, Bond Life Sciences Center, University of Missouri, Columbia, MO, USA. AD - Department of Surgery, School of Medicine, University of Missouri, Columbia, MO, USA. AD - Institute of Data Science and Informatics, University of Missouri, Columbia, MO, USA. FAU - Balakrishnan, Christopher N AU - Balakrishnan CN AUID- ORCID: 0000-0002-0788-0659 AD - Department of Biology, East Carolina University, Greenville, NC, USA. AD - Division of Environmental Biology, National Science Foundation, Alexandria, VA, USA. AD - Department of Vertebrate Zoology, National Museum of Natural History, Smithsonian Institution, Washington, DC, USA. LA - eng GR - DBI 1754311/National Science Foundation (NSF)/ GR - DBI 1754643/National Science Foundation (NSF)/ GR - DBI 1754397/National Science Foundation (NSF)/ GR - DBI 1940624/National Science Foundation (NSF)/ GR - DBI 1754406/National Science Foundation (NSF)/ PT - Journal Article DEP - 20261027 PL - England TA - Nat Ecol Evol JT - Nature ecology & evolution JID - 101698577 SB - IM COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:20 PHST- 2025/08/25 00:00 [received] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:20 [entrez] AID - 10.1038/s41559-026-03216-w [pii] AID - 10.1038/s41559-026-03216-w [doi] PST - aheadofprint SO - Nat Ecol Evol. 2026 Oct 27. doi: 10.1038/s41559-026-03216-w. PMID- 42946806 OWN - NLM STAT- Publisher LR - 20261028 IS - 1365-2540 (Electronic) IS - 0018-067X (Linking) DP - 2026 Oct 27 TI - Isolation to extinction: landscape fragmentation and connectivity in sloth bears of the Indian Terai. LID - 10.1038/s41437-026-00897-8 [doi] AB - Habitat fragmentation is a major global threat to biodiversity, leading to small and isolated populations. Fragmented landscapes can reduce connectivity, erode genetic diversity, and increase inbreeding risk, potentially contributing to local extinction. Using a landscape genetics approach, we investigated sloth bear (Melursus ursinus) populations in India's Central Terai landscape-a human-dominated, multi-use region that exemplifies the challenges of conserving wildlife populations in highly fragmented landscapes. Using non-invasively collected fecal samples (n = 209), we identified 66 individuals from 964 genome-wide SNPs generated via ddRAD sequencing. Spatial genetic structuring was not strictly congruent to the protected areas and genetic clusters showed low genetic diversity (mean H(E) = 0.156 ; mean H(O) = 0.079) with a heterozygote deficit (mean F(IS) = 0.323). Further, landscape genetic analyses revealed agriculture as the primary barrier to gene flow across the landscape, followed by proximity to water. Our findings establish the first genetic baseline for sloth bears in this region and suggest that historical population structure and contemporary fragmentation may jointly influence genetic connectivity and diversity across the landscape. We demonstrate the unique utility of non-invasive samples and genome-wide SNP based analyses for poorly studied species of conservation concern, despite experimental and bioinformatic challenges. Our novel insights underscores the importance of such genetic monitoring in fragmented landscapes. CI - (c) 2026. The Author(s), under exclusive licence to The Genetics Society. FAU - Rana, Divyashree AU - Rana D AUID- ORCID: 0000-0003-1981-5536 AD - National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India. divyashreerana24@gmail.com. FAU - Samjith, Judi Clarissa AU - Samjith JC AD - National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India. AD - Department of Biotechnology, Manipal Institute of Technology (MIT), Manipal Academy of Higher Education (MAHE), Manipal, India. FAU - Ramakrishnan, Uma AU - Ramakrishnan U AUID- ORCID: 0000-0002-5370-5966 AD - National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Heredity (Edinb) JT - Heredity JID - 0373007 SB - IM COIS- Competing interests: The authors declare no competing interests. Ethical statement: This article does not contain any studies with human participants or animals performed by any of the authors. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:20 PHST- 2025/10/24 00:00 [received] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:20 [entrez] AID - 10.1038/s41437-026-00897-8 [pii] AID - 10.1038/s41437-026-00897-8 [doi] PST - aheadofprint SO - Heredity (Edinb). 2026 Oct 27. doi: 10.1038/s41437-026-00897-8. PMID- 42946792 OWN - NLM STAT- Publisher LR - 20261028 IS - 1433-2965 (Electronic) IS - 0937-941X (Linking) DP - 2026 Oct 27 TI - Clinical role and timing of osteoporosis evaluation and treatment after distal radius fracture: a scoping review. LID - 10.1007/s00198-026-08296-9 [doi] AB - Distal radius fracture is an early fragility fracture, yet evidence guiding osteoporosis treatment afterward is unclear. In this scoping review of 250 reports, post-fracture osteoporosis care was associated with fewer subsequent fractures in observational studies, but no randomized distal radius fracture-specific trial exists and normal-bone-mineral-density patients remain understudied. PURPOSE: To map the extent, range, and nature of evidence on the clinical role and timing of osteoporosis evaluation and treatment after distal radius fracture (DRF). METHODS: We conducted a JBI-guided scoping review reported per PRISMA-ScR (prospectively registered on OSF). PubMed, OpenAlex, Europe PMC, Embase, the Cochrane Database of Systematic Reviews, KoreaMed, and CiNii were searched on 4 May 2026 for adults aged >/=50 years with DRF or wrist fragility fracture. Records underwent dual large language model screening, independently audited in duplicate by two authors, and source-verified charting, synthesized descriptively without pooling. RESULTS: Across 250 reports (2008-2026), evidence addressed BMD-based risk and selection (103), the care gap (65), prognosis (40), and treatment (42). Median DXA testing and treatment-initiation rates were 26% and 22%. Eleven observational reports linked treatment or structured care to fracture outcomes; a separate economic model projected outcomes. Estimates were heterogeneous and not pooled. No included study directly compared early versus delayed treatment initiation after DRF with subsequent fracture as the outcome. Six reports focused on normal BMD; none estimated subgroup-specific fracture outcomes. CONCLUSION: A fragility DRF warrants prompt secondary prevention, assessment for secondary causes, and timely indicated treatment. DXA and FRAX can inform individualized decisions but should not delay indicated care. Observational associations do not establish a causal treatment effect; optimal timing and benefit in normal-BMD patients remain unresolved. CI - (c) 2026. The Author(s), under exclusive licence to the International Osteoporosis Foundation and the Bone Health and Osteoporosis Foundation. FAU - Kwon, Bong Cheol AU - Kwon BC AUID- ORCID: 0000-0001-9777-9409 AD - Department of Orthopedic Surgery, Hallym University Sacred Heart Hospital, Hallym University College of Medicine, Anyang, Republic of Korea. bckwon11@gmail.com. FAU - Cho, Sungwoo AU - Cho S AUID- ORCID: 0000-0002-9998-1974 AD - Department of Orthopedic Surgery, Hallym University Sacred Heart Hospital, Hallym University College of Medicine, Anyang, Republic of Korea. FAU - Chae, Sungkuk AU - Chae S AUID- ORCID: 0009-0009-7421-4160 AD - Department of Orthopedic Surgery, Hallym University Sacred Heart Hospital, Hallym University College of Medicine, Anyang, Republic of Korea. FAU - Lee, Cheho AU - Lee C AUID- ORCID: 0009-0008-9081-0811 AD - Department of Orthopedic Surgery, Hallym University Dongtan Sacred Heart Hospital, Hallym University College of Medicine, Hwaseong, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - England TA - Osteoporos Int JT - Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA JID - 9100105 SB - IM OTO - NOTNLM OT - Bone mineral density OT - Distal radius fracture OT - Osteoporosis OT - Scoping review OT - Secondary fracture prevention COIS- Declarations. Conflict of interest: Bong Cheol Kwon, Sungwoo Cho, Sungkuk Chae, and Cheho Lee declare that they have no conflict of interest. This article does not contain any studies with human participants or animals performed by any of the authors. The manuscript does not contain clinical studies or patient data. Informed consent: Not applicable; this review analysed previously published studies and no individual participant data were used. Protocol registration: The review protocol was prospectively registered with the Open Science Framework on 4 May 2026, before the database searches were run (registration ID N3q9a; https://osf.io/N3q9a ). Ethical review statement: This study is a scoping review of previously published literature and did not involve human participants or individual patient data; institutional review board approval was therefore not required. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:19 PHST- 2026/08/08 00:00 [received] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:19 [entrez] AID - 10.1007/s00198-026-08296-9 [pii] AID - 10.1007/s00198-026-08296-9 [doi] PST - aheadofprint SO - Osteoporos Int. 2026 Oct 27. doi: 10.1007/s00198-026-08296-9. PMID- 42946791 OWN - NLM STAT- Publisher LR - 20261028 IS - 1546-1726 (Electronic) IS - 1097-6256 (Linking) DP - 2026 Oct 27 TI - Stimulus-specific recruitment of human amygdala neurons predicts episodic memory encoding success. LID - 10.1038/s41593-026-02493-9 [doi] AB - Controlling whether an experience is encoded into long-term memory and thus later remembered is a crucial aspect of memory. Here we examined single-neuron activity in the human brain while participants formed memories for novel visual stimuli and later retrieved them with confidence ratings. Category-selective amygdala neurons exhibited elevated firing during encoding of later remembered items relative to later forgotten items. This effect was predictive of the episodic aspect of the memory, was restricted to the preferred category of each cell, emerged earlier and was stronger in the amygdala compared to the hippocampus, and did not depend on stimulus valence and arousal. Successful memory formation was accompanied by enhanced spike-field coherence between the activity of amygdala category cells and hippocampal field potentials. These findings demonstrate that stimulus-specific recruitment of the amygdala predicts memory formation and suggest category cells in the right amygdala as a target for interventions to treat disorders affecting episodic memory in humans. CI - (c) 2026. The Author(s). FAU - Xiao, Jiayang AU - Xiao J AD - Department of Neurosurgery, Cedars-Sinai Medical Center, Los Angeles, CA, USA. AD - Department of Neurology, Cedars-Sinai Medical Center, Los Angeles, CA, USA. AD - Center for Neural Science and Medicine, Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA. FAU - Daume, Jonathan AU - Daume J AD - Department of Neurosurgery, Cedars-Sinai Medical Center, Los Angeles, CA, USA. AD - Department of Neurology, Cedars-Sinai Medical Center, Los Angeles, CA, USA. AD - Center for Neural Science and Medicine, Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA. FAU - Salimpour, Yousef AU - Salimpour Y AD - Department of Neurosurgery, Johns Hopkins School of Medicine, Baltimore, MD, USA. FAU - Kurilenko, Natalia AU - Kurilenko N AD - Department of Neurosurgery, Cedars-Sinai Medical Center, Los Angeles, CA, USA. AD - Department of Neurology, Cedars-Sinai Medical Center, Los Angeles, CA, USA. AD - Center for Neural Science and Medicine, Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA. FAU - Mosher, Clayton AU - Mosher C AD - Department of Neurosurgery, Cedars-Sinai Medical Center, Los Angeles, CA, USA. AD - Department of Neurology, Cedars-Sinai Medical Center, Los Angeles, CA, USA. AD - Center for Neural Science and Medicine, Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA. FAU - Anderson, William S AU - Anderson WS AUID- ORCID: 0000-0003-0958-050X AD - Department of Neurosurgery, Johns Hopkins School of Medicine, Baltimore, MD, USA. FAU - Valiante, Taufik A AU - Valiante TA AUID- ORCID: 0000-0002-3443-3790 AD - Krembil Research Institute, Toronto, Ontario, Canada. AD - Division of Neurosurgery, University of Toronto, Toronto, Ontario, Canada. AD - Department of Electrical and Computer Engineering, University of Toronto, Toronto, Ontario, Canada. FAU - Mamelak, Adam N AU - Mamelak AN AUID- ORCID: 0000-0002-4245-6431 AD - Department of Neurosurgery, Cedars-Sinai Medical Center, Los Angeles, CA, USA. FAU - Rutishauser, Ueli AU - Rutishauser U AUID- ORCID: 0000-0002-9207-7069 AD - Department of Neurosurgery, Cedars-Sinai Medical Center, Los Angeles, CA, USA. Ueli.Rutishauser@csmc.edu. AD - Department of Neurology, Cedars-Sinai Medical Center, Los Angeles, CA, USA. Ueli.Rutishauser@csmc.edu. AD - Center for Neural Science and Medicine, Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, USA. Ueli.Rutishauser@csmc.edu. AD - Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA. Ueli.Rutishauser@csmc.edu. LA - eng GR - U01NS117839/U.S. Department of Health & Human Services | NIH | National Institute of Neurological Disorders and Stroke (NINDS)/ PT - Journal Article DEP - 20261027 PL - United States TA - Nat Neurosci JT - Nature neuroscience JID - 9809671 SB - IM COIS- Competing interests: W.S.A. serves as a compensated consultant for iota Biosciences, UniQure B.V. and Solid Biosciences. He receives royalty payments from Globus Medical. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:19 PHST- 2025/10/21 00:00 [received] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:19 [entrez] AID - 10.1038/s41593-026-02493-9 [pii] AID - 10.1038/s41593-026-02493-9 [doi] PST - aheadofprint SO - Nat Neurosci. 2026 Oct 27. doi: 10.1038/s41593-026-02493-9. PMID- 42946785 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261028 IS - 1436-5073 (Electronic) IS - 0026-3672 (Linking) VI - 193 IP - 11 DP - 2026 Oct 28 TI - Single-atom nanozyme-enabled immunosensors: from atomic design to analytical applications. LID - 766 [pii] LID - 10.1007/s00604-026-08498-2 [doi] AB - Immunosensors achieve highly selective detection through specific antigen-antibody recognition. However, their signal-amplification strategies, which are commonly based on natural enzymes or noble-metal nanomaterials, often suffer from poor stability, high cost, and susceptibility to matrix interference. Although nanozymes have emerged as promising alternatives, their heterogeneous active domains and poorly defined electronic structures often result in less controllable uncontrollable catalytic behavior. Single-atom nanozymes (SANs), featuring atomically dispersed active sites and tunable coordination/electronic structures, establish well-defined structure-activity relationships and provide a mechanistic link between atomic-scale active-site engineering and sensing performance through integration with immunorecognition interfaces. In this review, recent advances in SAN-enabled immunosensors are systematically summarized from the perspective of the structure-mechanism-application relationship. We discuss active-site engineering strategies, characterization techniques, and catalytic mechanisms that govern enzyme-mimicking activity, followed by representative signal-amplification pathways and sensing platforms across different detection modes. Emerging applications in clinical diagnostics and food and agricultural safety are highlighted. Finally, the key challenges toward practical translation and future perspectives, including AI-guided materials discovery, mechanism-guided design, multifunctional sensing systems, and real-world deployment, are discussed. This review aims to provide fundamental design principles for the rational development of next-generation high-performance single-atom immunosensors. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature. FAU - Yue, Wangxiang AU - Yue W AD - College of Artificial Intelligence, Taiyuan University of Technology, Taiyuan, 030024, China. AD - School of Chemistry, Xi'an Jiaotong University, Xi'an, 710049, China. FAU - Wang, Tianqi AU - Wang T AD - College of Artificial Intelligence, Taiyuan University of Technology, Taiyuan, 030024, China. FAU - Fan, Xiujun AU - Fan X AD - School of Chemistry, Xi'an Jiaotong University, Xi'an, 710049, China. fxiujun@xjtu.edu.cn. FAU - Li, Haonan AU - Li H AD - College of Artificial Intelligence, Taiyuan University of Technology, Taiyuan, 030024, China. FAU - Wang, Zhihao AU - Wang Z AD - College of Materials Science and Engineering, Chongqing University, Chongqing, 400044, China. FAU - Li, Yongfeng AU - Li Y AD - College of Artificial Intelligence, Taiyuan University of Technology, Taiyuan, 030024, China. liyongfeng@tyut.edu.cn. LA - eng GR - 22278291/National Natural Science Foundation of China/ GR - 202203021211145/Natural Science Foundation of Shanxi Province/ PT - Journal Article PT - Review DEP - 20261028 PL - Austria TA - Mikrochim Acta JT - Mikrochimica acta JID - 7808782 SB - IM MH - *Biosensing Techniques/methods MH - Immunoassay/methods MH - *Nanostructures/chemistry MH - Humans MH - Catalysis MH - Catalytic Domain OTO - NOTNLM OT - Active-site engineering OT - Nanozymes OT - Signal amplification OT - Single-atom catalysts (SACs) OT - Single-atom immunosensors OT - Single-atom nanozymes (SANs) OT - Structure-activity relationship COIS- Declarations. Competing interests: The authors declare no competing interests. Artificial Intelligence Disclosure: Artificial intelligence (AI) tools (OpenAI ChatGPT) were used solely for language editing to improve the readability and grammatical quality of the manuscript. No AI tools were used for the preparation of figures or the graphical abstract. All scientific content, literature analysis, interpretation, and conclusions were developed, verified, and approved by the authors. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 CRDT- 2026/10/28 00:19 PHST- 2026/07/30 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:19 [entrez] AID - 10.1007/s00604-026-08498-2 [pii] AID - 10.1007/s00604-026-08498-2 [doi] PST - epublish SO - Mikrochim Acta. 2026 Oct 28;193(11):766. doi: 10.1007/s00604-026-08498-2. PMID- 42946751 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 28 TI - Phase-dependent Al leaching and crystal structure stability of CoAl(2)O(4) in alkaline media. LID - 10.1038/s41467-026-78099-3 [doi] LID - 11198 AB - Surface changes have attracted great interest in solid synthesis, catalysis and corrosion. Herein, we demonstrate a distinct surface Al/Co ratio-dependent reconstruction behavior in alkaline media for sol-gel-derived CoAl(2)O(4) particles. Our results indicate that the nominal Al/Co = 2 represents an average composition for particles formed at low temperatures, with spinel Al-excess surfaces and Al-poor cores. Annealing at high temperature homogenizes the cation distribution by the progressive incorporation of surface-Al into the core. Upon exposure to alkaline solution, the Al-excess surface phases are selectively leached and stabilize at a new spinel-type Al-rich surface. In contrast, no selective leaching is observed in normal spinel CoAl(2)O(4) with a homogeneously single phase. These unexpected findings highlight the surface-composition dependent phase dissolution and the progressive incorporation of atoms into specific crystal structures during multi-metal oxide synthesis, hence drawing attention to the neglected relay-like phase formation of each single compound before the final-phase formation of mixed metal compounds. CI - (c) 2026. The Author(s). FAU - Sun, Shengnan AU - Sun S AUID- ORCID: 0000-0001-5298-0301 AD - Institute of Materials Research and Engineering (IMRE), Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis #08-03, Singapore, 138634, Republic of Singapore. FAU - Tan, Hui Ru AU - Tan HR AD - Institute of Materials Research and Engineering (IMRE), Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis #08-03, Singapore, 138634, Republic of Singapore. FAU - Zhao, Peng AU - Zhao P AD - Institute of Advanced Intelligence and Computing (IAIC), Agency for Science, Technology and Research (A*STAR), 1 Fusionopolis Way, #16-16 Connexis, Singapore, 138632, Republic of Singapore. FAU - Xi, Shibo AU - Xi S AUID- ORCID: 0000-0002-8521-3237 AD - Institute of Sustainability for Chemicals, Energy and Environment (ISCE(2)), Agency for Science, Technology and Research (A*STAR), 1 Pesek Road, Jurong Island, Singapore, 627833, Republic of Singapore. xi_shibo@a-star.edu.sg. FAU - Wei, Fengxia AU - Wei F AD - Institute of Materials Research and Engineering (IMRE), Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis #08-03, Singapore, 138634, Republic of Singapore. AD - Mark Wainwright Analytical Center, University of New South Wales, Sydney, NSW, 2052, Australia. FAU - Seng, Debbie Hwee Leng AU - Seng DHL AD - Institute of Materials Research and Engineering (IMRE), Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis #08-03, Singapore, 138634, Republic of Singapore. FAU - Ren, Yi AU - Ren Y AD - Institute of Materials Research and Engineering (IMRE), Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis #08-03, Singapore, 138634, Republic of Singapore. FAU - Zhang, Jia AU - Zhang J AD - Institute of Advanced Intelligence and Computing (IAIC), Agency for Science, Technology and Research (A*STAR), 1 Fusionopolis Way, #16-16 Connexis, Singapore, 138632, Republic of Singapore. zhangj@a-star.edu.sg. FAU - Lin, Ming AU - Lin M AD - Institute of Materials Research and Engineering (IMRE), Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis #08-03, Singapore, 138634, Republic of Singapore. Lin_Ming@a-star.edu.sg. FAU - Seh, Zhi Wei AU - Seh ZW AUID- ORCID: 0000-0003-0953-567X AD - Institute of Materials Research and Engineering (IMRE), Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Innovis #08-03, Singapore, 138634, Republic of Singapore. sehzw@a-star.edu.sg. LA - eng GR - MTC Programmatic Fund M23L9b0052/Agency for Science, Technology and Research (A*STAR)/ PT - Journal Article DEP - 20260928 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 SB - IM PMC - PMC13713982 COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/09/28 CRDT- 2026/10/28 00:17 PHST- 2026/03/20 00:00 [received] PHST- 2026/09/08 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:17 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - 10.1038/s41467-026-78099-3 [pii] AID - 78099 [pii] AID - 10.1038/s41467-026-78099-3 [doi] PST - epublish SO - Nat Commun. 2026 Sep 28;17(1):11198. doi: 10.1038/s41467-026-78099-3. PMID- 42946749 OWN - NLM STAT- Publisher LR - 20261028 IS - 2055-0278 (Electronic) IS - 2055-0278 (Linking) DP - 2026 Oct 27 TI - Legacy effects of selective coppicing on forest regeneration in Bronze Age Italy. LID - 10.1038/s41477-026-02425-2 [doi] AB - Unambiguous evidence of sophisticated woodland and land-use management in Bronze Age southern Europe is presented from the Terramare culture in northern Italy. Archaeological layers dated to ~3,600 years ago reveal a society whose economy was structurally dependent on wood, used as both building material and fuel, defining what we term here a 'Wood Age' culture. High-resolution pollen sequences and stratigraphic data from key sites indicate that oak-dominated woodlands regenerated through long-term recurrent cycles of selective coppicing targeting specific broad-leaved trees (Quercus species, Carpinus betulus, Corylus avellana and Ostrya carpinifolia) and that these practices followed repeated cycles of cutting and regrowth under sustained human disturbance. The Terramare economy relied on a multifunctional agro-silvopastoral system integrating woodland management, crop rotation, animal husbandry and water control. Yet, selected woodland patches were maintained for fruit gathering and boundary marking, highlighting a conscious balance between exploitation and ecological awareness. This land-use strategy sustained the Terramare communities for centuries but also drove profound and partly irreversible transformations of soils and water systems, at times exceeding local sustainability thresholds. By revealing long-term, regeneration-driven forest management, our results challenge the assumption that many temperate lowland forests represent natural baselines, highlighting instead the deep ecological legacies of past human intervention. CI - (c) 2026. The Author(s). FAU - Mercuri, Anna Maria AU - Mercuri AM AUID- ORCID: 0000-0001-6138-4165 AD - Laboratorio di Palinologia e Paleobotanica, Dipartimento di Scienze della Vita, Universita degli Studi di Modena e Reggio Emilia, Modena, Italy. annamaria.mercuri@unimore.it. FAU - Florenzano, Assunta AU - Florenzano A AUID- ORCID: 0000-0003-4759-6406 AD - Laboratorio di Palinologia e Paleobotanica, Dipartimento di Scienze della Vita, Universita degli Studi di Modena e Reggio Emilia, Modena, Italy. assunta.florenzano@unimore.it. FAU - Clo, Eleonora AU - Clo E AD - Laboratorio di Palinologia e Paleobotanica, Dipartimento di Scienze della Vita, Universita degli Studi di Modena e Reggio Emilia, Modena, Italy. FAU - Zappa, Jessica AU - Zappa J AD - Laboratorio di Palinologia e Paleobotanica, Dipartimento di Scienze della Vita, Universita degli Studi di Modena e Reggio Emilia, Modena, Italy. FAU - Bosi, Giovanna AU - Bosi G AD - Laboratorio di Palinologia e Paleobotanica, Dipartimento di Scienze della Vita, Universita degli Studi di Modena e Reggio Emilia, Modena, Italy. FAU - Rottoli, Mauro AU - Rottoli M AD - Laboratorio di Archeobiologia, Musei Civici di Como, Como, Italy. FAU - Zerboni, Andrea AU - Zerboni A AUID- ORCID: 0000-0002-6844-8528 AD - Dipartimento di Scienze della Terra 'A. Desio', Universita degli Studi di Milano, Milan, Italy. FAU - Cardarelli, Andrea AU - Cardarelli A AD - Dipartimento di Scienze dell'Antichita, Universita degli Studi di Roma 'La Sapienza', Rome, Italy. FAU - Cremaschi, Mauro AU - Cremaschi M AD - Dipartimento di Scienze della Terra 'A. Desio', Universita degli Studi di Milano, Milan, Italy. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Nat Plants JT - Nature plants JID - 101651677 SB - IM COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:17 PHST- 2026/02/13 00:00 [received] PHST- 2026/09/08 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:17 [entrez] AID - 10.1038/s41477-026-02425-2 [pii] AID - 10.1038/s41477-026-02425-2 [doi] PST - aheadofprint SO - Nat Plants. 2026 Oct 27. doi: 10.1038/s41477-026-02425-2. PMID- 42946739 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 28 TI - Amorphous BaO-Al(2)O(3) encapsulation enables durable metal catalysts for ammonia decomposition. LID - 10.1038/s41467-026-78117-4 [doi] LID - 11182 AB - Confinement architectures stabilise active metal nanoparticles while preserving molecular accessibility, which proves crucial for heterogeneous catalysis. However, existing methods for preparing encapsulated catalysts such as mesoporous oxides, zeolites, and metal-organic frameworks are costly, complex, and often unreliable. Solid‑state reactions offer a promising alternative, yet conventional pathways yield thermodynamically stable phases that limit activity. Here, we propose a facile, template-free strategy that intercepts amorphous intermediates during solid‑state reactions to form encapsulation layers for active metals. We select ammonia decomposition reaction (ADR, 2NH(3) --> N(2) + 3H(2)) as the target catalytic reaction, which is a pivotal route to CO(x)‑free hydrogen and affords the high‑temperature, reducing environment that drives solid‑state transformations. During ADR conditions, commercial BaCO(3) and Al(2)O(3) act as solid‑state reactants, forming Al(2)O(3)-BaO encapsulation layers around Co or Ni nanoparticles. The layers effectively restrict nanoparticle growth. Amorphous Al(2)O(3) facilitates NH(3) adsorption, and amorphous BaO accelerates N(2)/H(2) release and enables N-H activation. As a result, the encapsulated catalyst delivers an intrinsic rate of 1,358 mmol H(2).g(metal)(-1).min(-1) that surpasses existing non‑noble metal catalysts, alongside excellent durability during 480 h. This approach offers a blueprint for constructing durable confinement architectures in encapsulated metal catalysts. CI - (c) 2026. The Author(s). FAU - Zhang, Yuan-Yi AU - Zhang YY AUID- ORCID: 0009-0004-2602-6900 AD - Key Laboratory for Colloid and Interface Chemistry, Key Laboratory of Special Aggregated Materials, School of Chemistry and Chemical Engineering, Shandong University, Jinan, China. FAU - Xu, Kai AU - Xu K AD - Key Laboratory for Colloid and Interface Chemistry, Key Laboratory of Special Aggregated Materials, School of Chemistry and Chemical Engineering, Shandong University, Jinan, China. FAU - Wang, Wei-Wei AU - Wang WW AUID- ORCID: 0000-0002-4471-2152 AD - Key Laboratory for Colloid and Interface Chemistry, Key Laboratory of Special Aggregated Materials, School of Chemistry and Chemical Engineering, Shandong University, Jinan, China. FAU - Liu, Hao-Xin AU - Liu HX AD - Department of Chemical Engineering, University College London, London, UK. FAU - Ma, Chao AU - Ma C AUID- ORCID: 0000-0001-8599-9340 AD - College of Materials Science and Engineering, Hunan University, Changsha, China. FAU - Liu, Jin-Cheng AU - Liu JC AD - Center for Rare Earth and Inorganic Functional Materials, School of Materials Science and Engineering & National Institute for Advanced Materials, Nankai University, Tianjin, China. liujincheng@nankai.edu.cn. FAU - Wang, Feng Ryan AU - Wang FR AUID- ORCID: 0000-0002-2475-606X AD - Department of Chemical Engineering, University College London, London, UK. ryan.wang@ucl.ac.uk. FAU - Jia, Chun-Jiang AU - Jia CJ AUID- ORCID: 0000-0002-4254-5100 AD - Key Laboratory for Colloid and Interface Chemistry, Key Laboratory of Special Aggregated Materials, School of Chemistry and Chemical Engineering, Shandong University, Jinan, China. jiacj@sdu.edu.cn. LA - eng GR - 22225110/National Natural Science Foundation of China (National Science Foundation of China)/ PT - Journal Article DEP - 20260928 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 SB - IM PMC - PMC13713983 COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/09/28 CRDT- 2026/10/28 00:16 PHST- 2026/02/04 00:00 [received] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:16 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - 10.1038/s41467-026-78117-4 [pii] AID - 78117 [pii] AID - 10.1038/s41467-026-78117-4 [doi] PST - epublish SO - Nat Commun. 2026 Sep 28;17(1):11182. doi: 10.1038/s41467-026-78117-4. PMID- 42946738 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 28 TI - Contact-electro-catalyzed direct hydroxylation of aromatic C-H bonds under ambient conditions. LID - 10.1038/s41467-026-78111-w [doi] LID - 11179 AB - The selective activation of inert aromatic C-H bonds for the synthesis of high-value phenols is of considerable scientific and industrial significance. Conventional free radical-based hydroxylation methods typically require ultraviolet irradiation or large amounts of sacrificial agents, which significantly limit their applicability. Here, we present a contact-electro-catalysis (CEC) approach that enables aromatic hydroxylation through hydroxyl radicals (.OH) and strong interfacial electric fields generated at the polytetrafluoroethylene (PTFE)-H(2)O interface via contact electrification. Using benzene as a model substrate, phenol was obtained via .OH-mediated oxidation, and a variety of substrates with diverse electronic properties underwent efficient hydroxylation. For example, the transformation of 4-hydroxybenzoic acid yielded hydroquinone and 3,4-dihydroxybenzoic acid with maximum yields of 72.45 and 210.90 mumol.g(cat)(-1) h(-1), respectively. Electron paramagnetic resonance (EPR) confirmed the presence of .OH, whereas nuclear magnetic resonance (NMR) and liquid chromatography-mass spectrometry (LC-MS) analyses identified the products, thereby supporting mechanistic investigations. This work extends the application of contact-electro-catalysis to organic synthesis, providing a sustainable alternative despite existing challenges in selectivity and scalability. CI - (c) 2026. The Author(s). FAU - Zhao, Jiawei AU - Zhao J AD - State Key Laboratory of Physical Chemistry of Solid Surfaces, iChEM, College of Chemistry and Chemical Engineering, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen University, Xiamen, PR China. FAU - Xu, Mengqian AU - Xu M AD - Institute of Frontier Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Qingdao, PR China. FAU - Wang, Yanjie AU - Wang Y AD - State Key Laboratory of Physical Chemistry of Solid Surfaces, iChEM, College of Chemistry and Chemical Engineering, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen University, Xiamen, PR China. FAU - Liang, Zihan AU - Liang Z AD - State Key Laboratory of Physical Chemistry of Solid Surfaces, iChEM, College of Chemistry and Chemical Engineering, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen University, Xiamen, PR China. FAU - Ye, Haobin AU - Ye H AD - State Key Laboratory of Physical Chemistry of Solid Surfaces, iChEM, College of Chemistry and Chemical Engineering, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen University, Xiamen, PR China. FAU - Luo, Jin AU - Luo J AD - State Key Laboratory of Physical Chemistry of Solid Surfaces, iChEM, College of Chemistry and Chemical Engineering, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen University, Xiamen, PR China. FAU - Zhao, Qianwei AU - Zhao Q AD - Henan Institute of Medical and Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, PR China. qianwzhao@zzu.edu.cn. FAU - Yang, Li AU - Yang L AUID- ORCID: 0000-0001-6570-5704 AD - Institute of Frontier Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Qingdao, PR China. yangli12@sdu.edu.cn. FAU - Deng, Wei-Qiao AU - Deng WQ AUID- ORCID: 0000-0002-3671-5951 AD - Institute of Frontier Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Qingdao, PR China. FAU - Fan, Feng Ru AU - Fan FR AUID- ORCID: 0000-0001-6474-471X AD - State Key Laboratory of Physical Chemistry of Solid Surfaces, iChEM, College of Chemistry and Chemical Engineering, Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province (IKKEM), Xiamen University, Xiamen, PR China. frfan@xmu.edu.cn. LA - eng GR - 22222305/National Natural Science Foundation of China (National Science Foundation of China)/ PT - Journal Article DEP - 20260928 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 SB - IM PMC - PMC13714319 COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/09/28 CRDT- 2026/10/28 00:16 PHST- 2026/02/27 00:00 [received] PHST- 2026/09/14 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:16 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - 10.1038/s41467-026-78111-w [pii] AID - 78111 [pii] AID - 10.1038/s41467-026-78111-w [doi] PST - epublish SO - Nat Commun. 2026 Sep 28;17(1):11179. doi: 10.1038/s41467-026-78111-w. PMID- 42946736 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 28 TI - "Descending Enkephalinergic neurons in Barrington's nucleus gate sex-biased control of micturition". LID - 10.1038/s41467-026-77949-4 [doi] LID - 11178 AB - Barrington's nucleus (Bar) is a brainstem hub essential for lower urinary tract (LUT) function, yet the molecular identity and functional specialization of its neuronal subtypes remain poorly defined. Here, we construct a single-nucleus transcriptional atlas of the murine Bar and identify an excitatory Penk-expressing population (Bar-Penk) critical for LUT regulation. Bar-Penk neurons are selectively active during voiding, when the external urethral sphincter (EUS) relaxes, and their optogenetic activation elicits time-locked suppression of EUS activity. Chemogenetic activation of these neurons induces a sex-specific, aberrant pattern of micturition, whereas targeted ablation impairs voluntary marking behavior in response to female cues. We observe multiple regions involved in visceromotor regulation and behavioral state control that project to Bar-Penk neurons, supporting their role as integrators of internal state and environmental context that drive urinary output. These findings provide new insights into the brainstem circuits that shape reflexive and voluntary micturition and highlight how discrete neuronal subtypes contribute to sexually dimorphic regulation of LUT function. CI - (c) 2026. The Author(s). FAU - Klymko, Nataliya AU - Klymko N AUID- ORCID: 0009-0001-6862-6865 AD - Division of Nephrology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA. FAU - Sartori, Andrea M AU - Sartori AM AUID- ORCID: 0000-0002-9571-0288 AD - Division of Nephrology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA. FAU - Leon, Mihoko AU - Leon M AD - Division of Nephrology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA. FAU - Seifert, Cassandra N AU - Seifert CN AD - Division of Nephrology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA. AD - University of Maryland School of Medicine, Baltimore, MD, USA. FAU - Lee, Richard AU - Lee R AD - Division of Nephrology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA. FAU - Mathai, John C AU - Mathai JC AD - Division of Nephrology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA. FAU - Verstegen, Anne M J AU - Verstegen AMJ AUID- ORCID: 0000-0001-9510-7211 AD - Division of Nephrology, Department of Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA. aversteg@bidmc.harvard.edu. LA - eng SI - GEO/GSE226809 GR - P20-DK119789/U.S. Department of Health & Human Services | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (National Institute of Diabetes & Digestive & Kidney Diseases)/ GR - R01-DK113030/U.S. Department of Health & Human Services | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (National Institute of Diabetes & Digestive & Kidney Diseases)/ GR - R01-DK125708/U.S. Department of Health & Human Services | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (National Institute of Diabetes & Digestive & Kidney Diseases)/ PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20260928 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 RN - 0 (Enkephalins) SB - IM MH - Animals MH - Female MH - *Urination/physiology MH - *Neurons/physiology/metabolism MH - Male MH - *Barrington's Nucleus/physiology/cytology/metabolism MH - Mice MH - *Enkephalins/metabolism MH - Urethra/physiology/innervation MH - Optogenetics MH - Sex Characteristics MH - Mice, Inbred C57BL PMC - PMC13714312 COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/09/28 CRDT- 2026/10/28 00:16 PHST- 2025/06/24 00:00 [received] PHST- 2026/09/07 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:16 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - 10.1038/s41467-026-77949-4 [pii] AID - 77949 [pii] AID - 10.1038/s41467-026-77949-4 [doi] PST - epublish SO - Nat Commun. 2026 Sep 28;17(1):11178. doi: 10.1038/s41467-026-77949-4. PMID- 42946732 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 28 TI - Characterization of post-mitotic DNA synthesis and its regulation in human cells. LID - 10.1038/s41467-026-78035-5 [doi] LID - 11186 AB - DNA replication stress promotes cancer development and is associated with DNA synthesis beyond S-phase. Replication stress-induced DNA lesions and under-replicated DNA can persist into G2 and mitosis, where they undergo mitotic DNA synthesis (MiDAS). After cell division, inherited DNA lesions are protected by genome caretaker proteins and show signs of post-mitotic DNA synthesis (post-MiDAS) in G1. The genomic regions that undergo post-MiDAS, the cellular factors involved, and the implications for genome integrity remain poorly understood. Here, we provide evidence for post-MiDAS activity at centromeres and telomeres in cancer cells employing alternative lengthening of telomeres (ALT) and present data suggesting that a subset of these fragile genomic regions can cluster during post-MiDAS in G1. We further show that post-MiDAS is promoted by local SUMOylation and BLM recruitment into ALT-associated PML bodies (APBs) and involves DNA damage tolerance factors and proteins associated with ALT and MiDAS. Consistent with G1-specific regulation, complementary quantitative proteomics and targeted siRNA screening reveal that APC/C, together with its G1 co-activator CDH1 but independent of its mitotic co-activator CDC20, restrains excessive post-MiDAS in G1. Finally, we show that CDH1 depletion selectively impairs the survival of ALT-positive cancer cell lines, indicating that post-MiDAS might represent a potential ALT-specific vulnerability. CI - (c) 2026. The Author(s). FAU - Vornberger, Julia AU - Vornberger J AUID- ORCID: 0000-0002-3139-8168 AD - Department of Molecular Mechanisms of Disease, University of Zurich (UZH), Zurich, Switzerland. AD - Molecular Life Sciences PhD Program, Life Science Zurich Graduate School, Zurich, Switzerland. FAU - Imhof, Ralph AU - Imhof R AD - Department of Molecular Mechanisms of Disease, University of Zurich (UZH), Zurich, Switzerland. FAU - Pasti, Virginia AU - Pasti V AD - Department of Molecular Mechanisms of Disease, University of Zurich (UZH), Zurich, Switzerland. AD - Molecular Life Sciences PhD Program, Life Science Zurich Graduate School, Zurich, Switzerland. FAU - Bianchi, Beatrice AU - Bianchi B AD - Department of Molecular Mechanisms of Disease, University of Zurich (UZH), Zurich, Switzerland. AD - Molecular Life Sciences PhD Program, Life Science Zurich Graduate School, Zurich, Switzerland. FAU - Wei, Mingxuan AU - Wei M AD - Department of Molecular Mechanisms of Disease, University of Zurich (UZH), Zurich, Switzerland. AD - Molecular Life Sciences PhD Program, Life Science Zurich Graduate School, Zurich, Switzerland. FAU - Straw, Jeannette Alisha AU - Straw JA AD - Department of Molecular Mechanisms of Disease, University of Zurich (UZH), Zurich, Switzerland. FAU - Manohar, Sandhya AU - Manohar S AUID- ORCID: 0000-0002-1322-3954 AD - Department of Molecular Mechanisms of Disease, University of Zurich (UZH), Zurich, Switzerland. FAU - Altmeyer, Matthias AU - Altmeyer M AUID- ORCID: 0000-0003-3780-1170 AD - Department of Molecular Mechanisms of Disease, University of Zurich (UZH), Zurich, Switzerland. matthias.altmeyer@uzh.ch. LA - eng GR - 197003/Schweizerischer Nationalfonds zur Forderung der Wissenschaftlichen Forschung (Swiss National Science Foundation)/ GR - 10000233/Schweizerischer Nationalfonds zur Forderung der Wissenschaftlichen Forschung (Swiss National Science Foundation)/ PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20260928 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 RN - EC 3.6.4.12 (RecQ Helicases) RN - 0 (Cadherins) RN - 0 (CDH1 protein, human) RN - EC 3.6.1.- (Bloom syndrome protein) RN - 0 (Antigens, CD) RN - EC 2.3.2.27 (Anaphase-Promoting Complex-Cyclosome) RN - 156288-95-8 (CDC20 protein, human) RN - 9007-49-2 (DNA) RN - 0 (Cell Cycle Proteins) RN - 0 (RNA, Small Interfering) RN - 0 (Cdc20 Proteins) SB - IM MH - Humans MH - *Mitosis/genetics MH - *DNA Replication MH - Telomere/metabolism/genetics MH - RecQ Helicases/metabolism/genetics MH - Cadherins/metabolism/genetics MH - Antigens, CD/metabolism MH - Sumoylation MH - Centromere/metabolism/genetics MH - Anaphase-Promoting Complex-Cyclosome/metabolism/genetics MH - Cell Line, Tumor MH - Telomere Homeostasis MH - G1 Phase/genetics MH - *DNA/biosynthesis MH - DNA Damage MH - Cell Cycle Proteins/metabolism MH - RNA, Small Interfering/metabolism MH - Cdc20 Proteins PMC - PMC13713988 COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/09/28 CRDT- 2026/10/28 00:16 PHST- 2026/01/05 00:00 [received] PHST- 2026/09/10 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:16 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - 10.1038/s41467-026-78035-5 [pii] AID - 78035 [pii] AID - 10.1038/s41467-026-78035-5 [doi] PST - epublish SO - Nat Commun. 2026 Sep 28;17(1):11186. doi: 10.1038/s41467-026-78035-5. PMID- 42946729 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 9 TI - An elite allele TaNPF7.6-A1(mod) enhances nitrogen use efficiency and grain yield in wheat. LID - 10.1038/s41467-026-76836-2 [doi] LID - 11177 AB - Breeding wheat varieties with a high nitrogen use efficiency (NUE) reduces both production costs and threats to the ecological environment. Here, using candidate-gene and genome-wide association studies, we identify TaNPF7.6-A1 as a positive regulator of NUE in wheat. A widely conserved polymorphism in the coding region of TaNPF7.6-A1 produces allele TaNPF7.6-A1(mod) with a higher transcription level and nitrate transport activity than TaNPF7.6-A1(lan), resulting in a greater NUE. We also reveal the mechanism by which TaEIL3-TaJAZ1-TaNPF7.6-A1 regulates NUE in wheat. TaNPF7.6-A1(mod) interacts more strongly with TaJAZ1 than TaNPF7.6-A1(lan) and promotes the retention of nuclear-localized TaJAZ1 in the cytoplasm. TaJAZ1 interacts with TaEIL3 and represses TaEIL3, binding to the TaNPF7.6-A1(mod) promoter, influencing allele expression and causing phenotypic differences. TaNPF7.6-A1(mod) also shows significant geographical distribution aggregation globally and undergoes continuous positive selection during the breeding process. Overall, this study provides molecular and evolutionary insights into the regulation of NUE. CI - (c) 2026. The Author(s). FAU - Jiang, Di AU - Jiang D AUID- ORCID: 0009-0007-9758-4653 AD - College of Agronomy, Henan Agricultural University, Zhengzhou, China. AD - National Engineering Laboratory of Wheat, Key Laboratory of Wheat Biology and Genetic Breeding in Central Huanghuai Area, Ministry of Agriculture, Henan Key Laboratory of Wheat Germplasm Resources Innovation and Improvement, Institute of Crops Germplasm Resources, Henan Academy of Agricultural Sciences, Zhengzhou, China. AD - The Shennong Laboratory, Zhengzhou, China. FAU - Peng, Chaojun AU - Peng C AUID- ORCID: 0000-0002-5574-3552 AD - National Engineering Laboratory of Wheat, Key Laboratory of Wheat Biology and Genetic Breeding in Central Huanghuai Area, Ministry of Agriculture, Henan Key Laboratory of Wheat Germplasm Resources Innovation and Improvement, Institute of Crops Germplasm Resources, Henan Academy of Agricultural Sciences, Zhengzhou, China. AD - The Shennong Laboratory, Zhengzhou, China. FAU - Li, Yan AU - Li Y AD - National Engineering Laboratory of Wheat, Key Laboratory of Wheat Biology and Genetic Breeding in Central Huanghuai Area, Ministry of Agriculture, Henan Key Laboratory of Wheat Germplasm Resources Innovation and Improvement, Institute of Crops Germplasm Resources, Henan Academy of Agricultural Sciences, Zhengzhou, China. AD - The Shennong Laboratory, Zhengzhou, China. FAU - Gong, Chen AU - Gong C AD - National Engineering Laboratory of Wheat, Key Laboratory of Wheat Biology and Genetic Breeding in Central Huanghuai Area, Ministry of Agriculture, Henan Key Laboratory of Wheat Germplasm Resources Innovation and Improvement, Institute of Crops Germplasm Resources, Henan Academy of Agricultural Sciences, Zhengzhou, China. AD - The Shennong Laboratory, Zhengzhou, China. FAU - Fang, Yuhui AU - Fang Y AD - National Engineering Laboratory of Wheat, Key Laboratory of Wheat Biology and Genetic Breeding in Central Huanghuai Area, Ministry of Agriculture, Henan Key Laboratory of Wheat Germplasm Resources Innovation and Improvement, Institute of Crops Germplasm Resources, Henan Academy of Agricultural Sciences, Zhengzhou, China. AD - The Shennong Laboratory, Zhengzhou, China. FAU - Zhang, Xingyu AU - Zhang X AD - College of Agronomy, Henan Agricultural University, Zhengzhou, China. FAU - Wang, Zhaoyang AU - Wang Z AD - College of Agronomy, Henan Agricultural University, Zhengzhou, China. AD - National Engineering Laboratory of Wheat, Key Laboratory of Wheat Biology and Genetic Breeding in Central Huanghuai Area, Ministry of Agriculture, Henan Key Laboratory of Wheat Germplasm Resources Innovation and Improvement, Institute of Crops Germplasm Resources, Henan Academy of Agricultural Sciences, Zhengzhou, China. AD - The Shennong Laboratory, Zhengzhou, China. FAU - Wang, Xinnan AU - Wang X AD - College of Agronomy, Henan Agricultural University, Zhengzhou, China. AD - National Engineering Laboratory of Wheat, Key Laboratory of Wheat Biology and Genetic Breeding in Central Huanghuai Area, Ministry of Agriculture, Henan Key Laboratory of Wheat Germplasm Resources Innovation and Improvement, Institute of Crops Germplasm Resources, Henan Academy of Agricultural Sciences, Zhengzhou, China. AD - The Shennong Laboratory, Zhengzhou, China. FAU - Hu, Lin AU - Hu L AD - National Engineering Laboratory of Wheat, Key Laboratory of Wheat Biology and Genetic Breeding in Central Huanghuai Area, Ministry of Agriculture, Henan Key Laboratory of Wheat Germplasm Resources Innovation and Improvement, Institute of Crops Germplasm Resources, Henan Academy of Agricultural Sciences, Zhengzhou, China. AD - The Shennong Laboratory, Zhengzhou, China. FAU - Qi, Xueli AU - Qi X AUID- ORCID: 0000-0002-9425-9798 AD - National Engineering Laboratory of Wheat, Key Laboratory of Wheat Biology and Genetic Breeding in Central Huanghuai Area, Ministry of Agriculture, Henan Key Laboratory of Wheat Germplasm Resources Innovation and Improvement, Institute of Crops Germplasm Resources, Henan Academy of Agricultural Sciences, Zhengzhou, China. xueliqi888@163.com. AD - The Shennong Laboratory, Zhengzhou, China. xueliqi888@163.com. FAU - Xu, Weigang AU - Xu W AUID- ORCID: 0000-0003-4705-0390 AD - College of Agronomy, Henan Agricultural University, Zhengzhou, China. xuwg1958@163.com. AD - National Engineering Laboratory of Wheat, Key Laboratory of Wheat Biology and Genetic Breeding in Central Huanghuai Area, Ministry of Agriculture, Henan Key Laboratory of Wheat Germplasm Resources Innovation and Improvement, Institute of Crops Germplasm Resources, Henan Academy of Agricultural Sciences, Zhengzhou, China. xuwg1958@163.com. AD - The Shennong Laboratory, Zhengzhou, China. xuwg1958@163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20260909 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 RN - 0 (Plant Proteins) RN - N762921K75 (Nitrogen) SB - IM MH - *Triticum/genetics/metabolism/growth & development MH - Alleles MH - *Plant Proteins/genetics/metabolism MH - *Nitrogen/metabolism MH - Gene Expression Regulation, Plant MH - *Edible Grain/genetics/metabolism/growth & development MH - Genome-Wide Association Study MH - Promoter Regions, Genetic MH - Plant Breeding PMC - PMC13714292 COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/09/09 CRDT- 2026/10/28 00:15 PHST- 2026/03/02 00:00 [received] PHST- 2026/08/07 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:15 [entrez] PHST- 2026/09/09 00:00 [pmc-release] AID - 10.1038/s41467-026-76836-2 [pii] AID - 76836 [pii] AID - 10.1038/s41467-026-76836-2 [doi] PST - epublish SO - Nat Commun. 2026 Sep 9;17(1):11177. doi: 10.1038/s41467-026-76836-2. PMID- 42946724 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 14 TI - Studying the failure mechanism in Li-metal electrode by a reversible fluorescence analysis methodology. LID - 10.1038/s41467-026-77648-0 [doi] LID - 11191 AB - Elucidating the failure mechanisms of Li metal electrodes is critical to accelerating the development of long cycle life Li metal batteries. However, it has not yet fully been realized so far due to the challenges associated with quantitative and qualitative characterization of solid electrolyte interphase on metal at large-area scale. Herein, we establish a relationship between solid byproducts and dead Li amounts in solid electrolyte interphase on Li metal by using fluorescence imaging and fluorescence titration method. The success of this methodology depends on the finding of a highly selective molecular probe, dilithium 3-hydroxy-2-naphthoate, with reversible change in fluorescence wavelength and intensity toward Li metal. Accordingly, the continuous generation of solid byproducts is acknowledged as potential underlying reason towards dead Li accumulation, determining the failure of Li electrode. These findings provide insights into the understanding of failure mechanism of Li metal electrode and design of strategies to improve its cycle life. CI - (c) 2026. The Author(s). FAU - Liu, Xiao AU - Liu X AD - Henan Key Laboratory of Function-Oriented Porous Materials, College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang, Henan, P. R. China. FAU - Zhang, Peng AU - Zhang P AUID- ORCID: 0000-0001-6654-4615 AD - Department of Chemistry, College of Sciences, Northeastern University, Shenyang, Liaoning, P. R. China. zhangpeng1@mail.neu.edu.cn. FAU - Guo, Yunpeng AU - Guo Y AUID- ORCID: 0009-0004-5956-0640 AD - Department of Chemistry, College of Sciences, Northeastern University, Shenyang, Liaoning, P. R. China. FAU - Xiong, Chuncen AU - Xiong C AD - Henan Key Laboratory of Function-Oriented Porous Materials, College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang, Henan, P. R. China. FAU - Fu, Hongru AU - Fu H AD - Henan Key Laboratory of Function-Oriented Porous Materials, College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang, Henan, P. R. China. FAU - Liu, Guilong AU - Liu G AD - Henan Key Laboratory of Function-Oriented Porous Materials, College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang, Henan, P. R. China. FAU - Sui, Dong AU - Sui D AD - Henan Key Laboratory of Function-Oriented Porous Materials, College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang, Henan, P. R. China. FAU - Liu, Xianming AU - Liu X AD - Henan Key Laboratory of Function-Oriented Porous Materials, College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang, Henan, P. R. China. FAU - Ma, Lufang AU - Ma L AD - Henan Key Laboratory of Function-Oriented Porous Materials, College of Chemistry and Chemical Engineering, Luoyang Normal University, Luoyang, Henan, P. R. China. FAU - Wen, Liping AU - Wen L AUID- ORCID: 0000-0001-8546-8988 AD - Key Laboratory of Bio-inspired Smart Interface Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, China. FAU - Zhao, Yong AU - Zhao Y AUID- ORCID: 0000-0002-5039-4576 AD - School of Nanoscience and Materials Engineering, Henan University, Kaifeng, P. R. China. zhaoyong@henu.edu.cn. LA - eng GR - 22309073/National Natural Science Foundation of China (National Science Foundation of China)/ GR - U22A20437/National Natural Science Foundation of China (National Science Foundation of China)/ GR - 24A150025/Foundation of Henan Educational Committee (Foundation of Education Committee of Henan Province)/ GR - 24HASTIT006/Foundation of Henan Educational Committee (Foundation of Education Committee of Henan Province)/ GR - 24IRTSTHN001/Foundation of Henan Educational Committee (Foundation of Education Committee of Henan Province)/ GR - 2024SKL-A-012/State Key Laboratory of Catalysis (State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics)/ GR - 222301420005/Science and Technology Department of Henan Province (Henan Provincial Department of Science and Technology)/ PT - Journal Article DEP - 20260914 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 SB - IM PMC - PMC13714256 COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/09/14 CRDT- 2026/10/28 00:15 PHST- 2025/10/16 00:00 [received] PHST- 2026/08/28 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:15 [entrez] PHST- 2026/09/14 00:00 [pmc-release] AID - 10.1038/s41467-026-77648-0 [pii] AID - 77648 [pii] AID - 10.1038/s41467-026-77648-0 [doi] PST - epublish SO - Nat Commun. 2026 Sep 14;17(1):11191. doi: 10.1038/s41467-026-77648-0. PMID- 42946721 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2041-4889 (Electronic) VI - 17 IP - 1 DP - 2026 Aug 24 TI - From injury to degeneration: delayed lipid-mediated neurotoxicity in a human astrocyte traumatic brain injury in-a-dish model. LID - 10.1038/s41419-026-09217-8 [doi] LID - 911 AB - Traumatic brain injury (TBI) is a major cause of long-term neurological disability, and repeated mild TBI (rmTBI) is increasingly linked to chronic neurodegeneration. Clinical evidence suggests that rmTBI creates a prolonged window of vulnerability to further injury. However, the astrocyte-intrinsic mechanisms that convert repeated mechanical stress into delayed neuronal injury remain poorly defined. In particular, whether rmTBI induces a distinct lipid-mediated neurotoxic programme in human astrocytes is unknown. Using a human astrocyte in vitro platform of rmTBI, we deliver controlled repeated mild injuries to astrocytes. Repeated injury induces a distinctive astrocytic phenotype characterised by cytoskeletal remodelling, reactive oxygen species accumulation, biphasic volume changes, extracellular matrix remodelling and lipid droplet formation. Shotgun lipidomics of astrocyte-conditioned medium reveals selective enrichment of bioactive diacylglycerols (DG) following rmTBI. When applied to human cortical neurons, this conditioned medium impairs neuronal function and morphology, increases apoptosis and activates protein kinase C delta (PKCdelta). Pharmacological inhibition of PKC attenuates the neurotoxic effect of rmTBI astrocyte-conditioned medium. These findings support a model in which human astrocytes retain a form of 'injury memory' through sustained phenotypic and lipid metabolic remodelling. By implicating the DG-PKCdelta axis in delayed neurotoxicity, the study identifies PKC signalling as a tractable target for limiting the long-term consequences of rmTBI. Repeated mild traumatic brain injury induces astrocytic dysfunction, metabolic imbalance and disrupts lipid homeostasis. This leads to the aberrant release of specific diacylglycerol species which activate protein kinase C signalling in neurons and drive delayed neurotoxic cascades. CI - (c) 2026. The Author(s). FAU - Schlotterose, Luise AU - Schlotterose L AD - Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, UK. AD - British Heart Foundation (BHF) - UK Dementia Research Institute (UK DRI) Centre for Vascular Dementia Research (CVDR) at the University of Oxford, Oxford, UK. AD - Kavli Institute for NanoScience Discovery, University of Oxford, Oxford, UK. AD - British Heart Foundation (BHF) Oxford Centre of Research Excellence, University of Oxford, Oxford, UK. FAU - Struwe, Michel A AU - Struwe MA AD - Department for Structural Biology, Kiel University, Kiel, Germany. FAU - Scheidig, Axel J AU - Scheidig AJ AD - Department for Structural Biology, Kiel University, Kiel, Germany. FAU - Blomberg, Niek AU - Blomberg N AD - Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands. FAU - Giera, Martin AU - Giera M AUID- ORCID: 0000-0003-1684-1894 AD - Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands. FAU - Hattermann, Kirsten AU - Hattermann K AD - Institute of Anatomy, Kiel University, Kiel, Germany. FAU - Salman, Mootaz M AU - Salman MM AUID- ORCID: 0000-0002-5683-1706 AD - Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, UK. mootaz.salman@dpag.ox.ac.uk. AD - British Heart Foundation (BHF) - UK Dementia Research Institute (UK DRI) Centre for Vascular Dementia Research (CVDR) at the University of Oxford, Oxford, UK. mootaz.salman@dpag.ox.ac.uk. AD - Kavli Institute for NanoScience Discovery, University of Oxford, Oxford, UK. mootaz.salman@dpag.ox.ac.uk. AD - British Heart Foundation (BHF) Oxford Centre of Research Excellence, University of Oxford, Oxford, UK. mootaz.salman@dpag.ox.ac.uk. LA - eng GR - MR/W027119/1/RCUK | Medical Research Council (MRC)/ GR - RE/24/130024/BHF Centre of Research Excellence, Oxford (BHF Centre of Research Excellence in Oxford)/ GR - NIF\R1\242594/Royal Society/ PT - Journal Article DEP - 20260824 PL - England TA - Cell Death Dis JT - Cell death & disease JID - 101524092 RN - 0 (Diglycerides) RN - EC 2.7.11.13 (Protein Kinase C-delta) RN - 0 (Reactive Oxygen Species) RN - 0 (Culture Media, Conditioned) SB - IM MH - Humans MH - *Astrocytes/metabolism/pathology/drug effects MH - *Brain Injuries, Traumatic/pathology/metabolism MH - Neurons/metabolism/pathology/drug effects MH - Diglycerides/metabolism MH - Protein Kinase C-delta/metabolism MH - Reactive Oxygen Species/metabolism MH - Culture Media, Conditioned/pharmacology MH - Apoptosis/drug effects MH - Cells, Cultured MH - Lipid Metabolism MH - Models, Biological PMC - PMC13713977 COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/08/24 CRDT- 2026/10/28 00:15 PHST- 2026/05/28 00:00 [received] PHST- 2026/08/17 00:00 [accepted] PHST- 2026/07/30 00:00 [revised] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:15 [entrez] PHST- 2026/08/24 00:00 [pmc-release] AID - 10.1038/s41419-026-09217-8 [pii] AID - 9217 [pii] AID - 10.1038/s41419-026-09217-8 [doi] PST - epublish SO - Cell Death Dis. 2026 Aug 24;17(1):911. doi: 10.1038/s41419-026-09217-8. PMID- 42946719 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2041-4889 (Electronic) VI - 17 IP - 1 DP - 2026 Sep 2 TI - Survival of cancer cells following taxol-induced apoptosis is supported by ZEB1-mediated ferroptosis sensitivity. LID - 10.1038/s41419-026-09242-7 [doi] LID - 912 AB - Chemotherapy remains the mainstay treatment of many solid cancers; however, resistance and recurrence compromise patient outcomes. We investigated chemotherapy resistance mechanisms in Triple Negative Breast Cancer (TNBC) by examining cells that survive chemotherapy-induced apoptotic caspase activation. Using a biosensor that permanently labels cells surviving executioner caspase activation with GFP, we identified a GFP+ population that survives transient paclitaxel treatment. These GFP+ cells display a stem-like invasive phenotype, altered mitochondrial metabolism, and increased drug resistance. Metabolomics profiling identified defects in TCA cycle metabolites and accumulation of polyunsaturated fatty acids (PUFAs). Mechanistically, surviving GFP+ cells exhibited Epithelial to Mesenchymal Transition (EMT) and ZEB1-regulated repression of GPX4 expression, resulting in increased sensitivity to ferroptotic cell death. Consistent with this, transforming growth factor- beta induced EMT also suppressed GPX4 and increased ferroptosis sensitivity in Normal Mouse Mammary epithelial cells. ZEB1 knockdown strongly reduced the GFP+ surviving fraction, identifying ZEB1 as a key regulator of apoptotic survival. Supplementation with arachidonic acid restored survival in ZEB1 knockdown cells, while the ferroptosis-protective drug, Ferrostatin-1, prevented the accumulation of GFP+ survivors, demonstrating that ferroptosis sensitivity is required for the survival of TNBC cells from chemotherapy-induced apoptosis. Together, these findings reveal a previously unrecognized link between ferroptosis and apoptosis and identify potential biomarkers and therapeutic vulnerabilities in a subset of chemotherapy-resistant TNBC. CI - (c) 2026. The Author(s). FAU - Hausman, Rachel AU - Hausman R AUID- ORCID: 0009-0009-1642-4872 AD - Department of Basic and Translational Research, BC Cancer Research Institute, Vancouver, BC, Canada. AD - Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada. FAU - Brown, Wells S AU - Brown WS AD - Department of Basic and Translational Research, BC Cancer Research Institute, Vancouver, BC, Canada. FAU - Awrey, Shannon J AU - Awrey SJ AUID- ORCID: 0000-0001-9905-6853 AD - Department of Basic and Translational Research, BC Cancer Research Institute, Vancouver, BC, Canada. FAU - Tavakoli, Hossein AU - Tavakoli H AUID- ORCID: 0000-0003-0856-1649 AD - Department of Basic and Translational Research, BC Cancer Research Institute, Vancouver, BC, Canada. FAU - Montell, Denise J AU - Montell DJ AD - Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Barbara, CA, USA. FAU - McDonald, Paul C AU - McDonald PC AUID- ORCID: 0000-0002-7238-2912 AD - Department of Basic and Translational Research, BC Cancer Research Institute, Vancouver, BC, Canada. FAU - Dedhar, Shoukat AU - Dedhar S AUID- ORCID: 0000-0003-4355-1657 AD - Department of Basic and Translational Research, BC Cancer Research Institute, Vancouver, BC, Canada. sdedhar@bccrc.ca. AD - Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada. sdedhar@bccrc.ca. LA - eng GR - FDN-143318/Gouvernement du Canada | Canadian Institutes of Health Research (Instituts de Recherche en Sante du Canada)/ GR - 25077/Cancer Research Society (Societe de Recherche sur le Cancer)/ PT - Journal Article DEP - 20260902 PL - England TA - Cell Death Dis JT - Cell death & disease JID - 101524092 RN - P88XT4IS4D (Paclitaxel) RN - 0 (Zinc Finger E-box-Binding Homeobox 1) RN - 0 (ZEB1 protein, human) SB - IM MH - *Paclitaxel/pharmacology MH - *Zinc Finger E-box-Binding Homeobox 1/metabolism/genetics MH - *Ferroptosis/drug effects MH - Humans MH - Animals MH - *Apoptosis/drug effects MH - Female MH - Cell Line, Tumor MH - Mice MH - Epithelial-Mesenchymal Transition/drug effects MH - *Triple Negative Breast Neoplasms/pathology/metabolism/drug therapy/genetics MH - Drug Resistance, Neoplasm/drug effects MH - Cell Survival/drug effects PMC - PMC13714279 COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/09/02 CRDT- 2026/10/28 00:15 PHST- 2025/06/23 00:00 [received] PHST- 2026/08/25 00:00 [accepted] PHST- 2026/08/06 00:00 [revised] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:15 [entrez] PHST- 2026/09/02 00:00 [pmc-release] AID - 10.1038/s41419-026-09242-7 [pii] AID - 9242 [pii] AID - 10.1038/s41419-026-09242-7 [doi] PST - epublish SO - Cell Death Dis. 2026 Sep 2;17(1):912. doi: 10.1038/s41419-026-09242-7. PMID- 42946709 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 2041-1723 (Electronic) IS - 2041-1723 (Linking) VI - 17 IP - 1 DP - 2026 Sep 28 TI - Synthesis of 4- and 5-membered fluorine-stabilised semi-saturated heterocycles via alkylidene carbenes. LID - 10.1038/s41467-026-77771-y [doi] LID - 11200 AB - Heterocycles are ubiquitous in biologically active molecules and central to pharmaceutical design. Their diverse reactivity and three-dimensional architectures make them essential scaffolds for tuning non-covalent interactions with biological targets. Saturated and unsaturated heterocycles are well established and widely exploited, but semi-saturated variants are rare, limited by synthetic inaccessibility and oxidative instability. Here we present a general strategy for synthesising fluorinated semi-saturated four- and five-membered nitrogen and oxygen heterocycles via alkylidene carbene intermediates, and show through kinetic, voltammetry and metabolism studies that fluorine is key to stabilising these underexplored motifs. The method affords oxidatively stable products with diastereoselectivity across diverse substitution patterns and functional groups. These scaffolds can be selectively derivatised to yield saturated, unsaturated, and semi-saturated fluorinated analogues to provide broader access to pharmaceutically-relevant chemical space. CI - (c) 2026. The Author(s). FAU - Harvey Randle, E AU - Harvey Randle E AD - School of Chemistry, University of Bristol, Bristol, UK. FAU - Sedikides, Alexi T AU - Sedikides AT AD - School of Chemistry, University of Bristol, Bristol, UK. FAU - Lefranc, Julien AU - Lefranc J AD - Merck KGaA, Darmstadt, Germany. FAU - Lennox, Alastair J J AU - Lennox AJJ AUID- ORCID: 0000-0003-2019-7421 AD - School of Chemistry, University of Bristol, Bristol, UK. a.lennox@bristol.ac.uk. LA - eng GR - University Research Fellowship and Enhancement Awards/Royal Society/ GR - 949821/EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)/ GR - UKRI2898/RCUK | Engineering and Physical Sciences Research Council (EPSRC)/ GR - EP/S024107/1/RCUK | Engineering and Physical Sciences Research Council (EPSRC)/ PT - Journal Article DEP - 20260928 PL - England TA - Nat Commun JT - Nature communications JID - 101528555 SB - IM PMC - PMC13714270 COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/09/28 CRDT- 2026/10/28 00:14 PHST- 2026/04/16 00:00 [received] PHST- 2026/09/01 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:14 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - 10.1038/s41467-026-77771-y [pii] AID - 77771 [pii] AID - 10.1038/s41467-026-77771-y [doi] PST - epublish SO - Nat Commun. 2026 Sep 28;17(1):11200. doi: 10.1038/s41467-026-77771-y. PMID- 42946704 OWN - NLM STAT- Publisher LR - 20261028 IS - 1545-1550 (Electronic) IS - 1526-6028 (Linking) DP - 2026 Oct 27 TI - Minimally Invasive Management of Cardiac Tamponade Complicating Transcatheter Aortic Valve Implantation: A Technical Note. PG - 15266028261499962 LID - 10.1177/15266028261499962 [doi] AB - PURPOSE: Cardiac tamponade is an uncommon but potentially catastrophic complication of transcatheter aortic valve implantation (TAVI), particularly when caused by iatrogenic hemopericardium, with high mortality among patients requiring emergency cardiac surgery. We describe a structured minimally invasive subxiphoid protocol modification for immediate intraprocedural tamponade management and place this experience in the context of current evidence. MATERIALS AND METHODS: Between January 2023 and April 2026, cardiac tamponade developed intra- or peri-procedurally in 4 consecutive patients among approximately 350 TAVI procedures at a single tertiary center. The protocol comprised immediate subxiphoid decompression; insertion of two 16 Fr radiopaque Redon drains, placed anteriorly and posteriorly, each connected to a separate Drainobag bottle sustaining high negative pressure; hermetic layered closure; mechanical ventilation under sedation after decompression for at least 12 hours and progressive drain removal after 72 hours. RESULTS: All 4 patients achieved immediate hemodynamic recovery without conversion to emergency sternotomy. No hospital deaths or procedure-related complications occurred. No late pericardial reaccumulation, constrictive pericarditis or ventricular pseudoaneurysm occurred. CONCLUSION: In this small single-center series, dual-drain subxiphoid drainage with continuous high-vacuum aspiration was feasible and associated with hemodynamic recovery without emergency sternotomy in 4 selected TAVI patients. Larger comparative studies are required before broader use can be recommended. These observations should be considered hypothesis-generating.Clinical ImpactCardiac tamponade during TAVI requires rapid, mechanism-directed treatment. Image-guided pericardiocentesis remains the usual first drainage step, whereas ongoing iatrogenic hemopericardium or failure to stabilize requires immediate surgical escalation. In 4 carefully selected patients, dual-drain subxiphoid decompression with continuous high-vacuum aspiration restored hemodynamic stability without emergency sternotomy. These findings support its feasibility as a rescue option in carefully selected cases, while larger comparative studies are needed to define its hemostatic effectiveness and its role relative to conventional drainage. Persistent bleeding or recurrent hemodynamic instability should prompt surgical conversion. FAU - Costache, Victor Sebastian AU - Costache VS AUID- ORCID: 0009-0003-2339-3214 AD - Department of Cardiovascular Surgery, Faculty of Medicine, Titu Maiorescu University, Bucharest, Romania. AD - The Academy of Romanian Scientists, Bucharest, Romania. AD - Sanador Clinical Hospital, Bucharest, Romania. FAU - Costache, Andreea AU - Costache A AD - Department of Cardiovascular Surgery, Faculty of Medicine, Titu Maiorescu University, Bucharest, Romania. AD - Sanador Clinical Hospital, Bucharest, Romania. FAU - Oprea, Stefan Andrei AU - Oprea SA AD - Sanador Clinical Hospital, Bucharest, Romania. AD - Doctoral Studies, Faculty of Medicine, Titu Maiorescu University, Bucharest, Romania. FAU - Marinescu, Andra-Denis AU - Marinescu AD AUID- ORCID: 0009-0000-5810-0096 AD - Doctoral Studies, Faculty of Medicine, Titu Maiorescu University, Bucharest, Romania. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Endovasc Ther JT - Journal of endovascular therapy : an official journal of the International Society of Endovascular Specialists JID - 100896915 SB - IM OTO - NOTNLM OT - cardiac tamponade OT - high-vacuum drainage OT - left ventricular perforation OT - subxiphoid pericardial drainage OT - transcatheter aortic valve implantation EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:14 PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:14 [entrez] AID - 10.1177/15266028261499962 [doi] PST - aheadofprint SO - J Endovasc Ther. 2026 Oct 27:15266028261499962. doi: 10.1177/15266028261499962. PMID- 42946699 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2158-3188 (Electronic) IS - 2158-3188 (Linking) VI - 16 IP - 1 DP - 2026 Jul 31 TI - A canonical microcircuit for estimating Excitation/Inhibition (E/I) balance. LID - 10.1038/s41398-026-04312-y [doi] LID - 588 AB - Excitation/inhibition (E/I) balance is crucial for maintaining healthy brain function and can be disrupted in various neurological and psychiatric disorders. Despite its importance, there are few tools to study E/I balance non-invasively in humans. Here, we propose a canonical microcircuit model to estimate E/I balance from non-invasive magnetoencephalography (MEG), electroencephalography (EEG) or optically pumped magnetometers (OPM) recordings by parameterising global pyramidal and inhibitory cell excitability. We first establish that E/I parameters are identifiable and recoverable. We then explore the effects of these new parameters and their interaction with other parameters in a series of simulations. To highlight the clinical relevance of this new model, we simulate changes in E/I balance and their impact on event-related potentials (ERPs) derived from paired-click, passive and active oddball paradigms, which are among the most robust clinical biomarkers of schizophrenia. Our simulations show that a loss of pyramidal cell excitability can explain reduced ERP amplitudes across all three paradigms, mirroring empirical findings in schizophrenia. This method may serve as a computational assay for estimating synaptopathy and E/I balance from non-invasive electrophysiological recordings across various clinical conditions thereby advancing efforts to develop personalised interventions to restore E/I balance. CI - (c) 2026. The Author(s). FAU - Hauke, Daniel J AU - Hauke DJ AUID- ORCID: 0000-0003-1772-9239 AD - Hawkes Institute, Department of Computer Science, University College London, London, UK. daniel.hauke@kcl.ac.uk. AD - Department of Psychosis Studies, Institute of Psychiatry, Psychology and Neuroscience, King's College London, London, UK. daniel.hauke@kcl.ac.uk. AD - Department of Biostatistics & Health Informatics, Institute of Psychiatry, Psychology and Neuroscience, King's College London, London, UK. daniel.hauke@kcl.ac.uk. AD - King's Institute for Artificial Intelligence, King's College London, London, UK. daniel.hauke@kcl.ac.uk. FAU - Rodriguez-Sanchez, Julia AU - Rodriguez-Sanchez J AUID- ORCID: 0000-0001-5839-0230 AD - Hawkes Institute, Department of Computer Science, University College London, London, UK. AD - Department of Psychology and Neuroscience, City St George's, University of London, London, UK. FAU - Oloye, Hope AU - Oloye H AD - Hawkes Institute, Department of Computer Science, University College London, London, UK. FAU - Berndt, Lioba C S AU - Berndt LCS AUID- ORCID: 0009-0006-2455-3031 AD - Hawkes Institute, Department of Computer Science, University College London, London, UK. AD - Department of Psychology, Faculty of Health and Life Sciences, University of Exeter, Exeter, UK. AD - School of Physiology, Pharmacology and Neuroscience, University of Bristol, Bristol, UK. FAU - Pinotsis, Dimitris A AU - Pinotsis DA AD - Department of Psychology and Neuroscience, City St George's, University of London, London, UK. FAU - Friston, Karl J AU - Friston KJ AUID- ORCID: 0000-0001-7984-8909 AD - Department of Imaging Neuroscience, Queen Square Institute of Neurology, University College London, London, UK. FAU - Mathalon, Daniel H AU - Mathalon DH AUID- ORCID: 0000-0001-6090-4974 AD - Department of Psychiatry & Behavioral Sciences, University of California, San Francisco, CA, USA. AD - Mental Health Service, San Francisco Veterans Affairs Health Care System, San Francisco, CA, USA. FAU - Adams, Rick A AU - Adams RA AUID- ORCID: 0000-0002-7661-8881 AD - Institute of Cognitive Neuroscience, University College London, London, UK. LA - eng GR - 226793/Z/22/Z/Wellcome Trust (Wellcome)/ PT - Journal Article DEP - 20260731 PL - United States TA - Transl Psychiatry JT - Translational psychiatry JID - 101562664 SB - IM MH - Humans MH - Magnetoencephalography MH - Electroencephalography MH - *Neural Inhibition/physiology MH - *Schizophrenia/physiopathology MH - *Evoked Potentials/physiology MH - *Models, Neurological MH - *Brain/physiology/physiopathology MH - *Pyramidal Cells/physiology MH - Computer Simulation PMC - PMC13714252 COIS- Competing interests: The authors declare no competing interests. Ethics approval and consent to participate: Both, the B-SNIP and NAPLS2 study were approved by the institutional review board at every participating institution. Adult participants provided written informed consent. Minors provided written assent and their parents providing written informed consent. All methods were performed in accordance with the relevant guidelines and regulations. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/07/31 CRDT- 2026/10/28 00:13 PHST- 2025/08/12 00:00 [received] PHST- 2026/07/24 00:00 [accepted] PHST- 2026/04/22 00:00 [revised] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:13 [entrez] PHST- 2026/07/31 00:00 [pmc-release] AID - 10.1038/s41398-026-04312-y [pii] AID - 4312 [pii] AID - 10.1038/s41398-026-04312-y [doi] PST - epublish SO - Transl Psychiatry. 2026 Jul 31;16(1):588. doi: 10.1038/s41398-026-04312-y. PMID- 42946698 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2059-3635 (Electronic) IS - 2095-9907 (Print) IS - 2059-3635 (Linking) VI - 11 IP - 1 DP - 2026 Oct 28 TI - Ginsenoside Rb1 alleviates uterine aging via AMPK-mediated mTOR inactivation. LID - 10.1038/s41392-026-02970-4 [doi] LID - 472 AB - Uterine aging impairs fertility and reproductive health, but the underlying pathogenic mechanisms and effective treatment strategies remain elusive. Defective endometrial decidualization and implantation failure are hallmarks of uterine aging, which can be driven by cellular senescence, especially in endometrial stromal cells (EnSCs). Rb1 has been reported to relieve cellular senescence by suppressing oxidative and inflammatory pathways. However, whether Rb1 functions in alleviating uterine aging remains uncertain. Here, we demonstrate that Rb1 can attenuate oxidative stress, senescence, and apoptosis in the EnSCs of the aging human endometrium and murine uterus. Further proteomic analysis reveals that Rb1 safeguards against endometrial aging by regulating mammalian target of rapamycin (mTOR)-related pathways. Rb1 treatment results in the activation of AMP-activated protein kinase (AMPK) and AMPK-mediated suppression of the mTOR-nuclear factor-kappa B (NF-kappaB) signaling loop, thereby ameliorating aging-related phenotypes. Collectively, our findings illustrate that Rb1 alleviates uterine aging by attenuating endometrial stromal cell senescence and suppressing AMPK-mTOR-NF-kappaB-driven inflammatory signaling. Rather than selectively eliminating senescent cells, Rb1 primarily targets senescence-associated dysfunction and restores endometrial homeostasis, highlighting its potential as a senescence-modulating therapeutic strategy for uterine aging. CI - (c) 2026. The Author(s). FAU - Zhou, Ping AU - Zhou P AD - State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China. AD - Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China. AD - Beijing Key Laboratory of Collaborative Innovation in Frontier Technologies for Population Quality, Beijing, China. AD - National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China. AD - National Clinical Key Specialty Construction Program, P.R. China (2023), Beijing, China. FAU - Wang, Yue AU - Wang Y AUID- ORCID: 0000-0002-0737-6479 AD - State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China. AD - Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China. AD - Beijing Key Laboratory of Collaborative Innovation in Frontier Technologies for Population Quality, Beijing, China. AD - National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China. AD - National Clinical Key Specialty Construction Program, P.R. China (2023), Beijing, China. FAU - Cheng, Ming AU - Cheng M AD - State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China. AD - Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China. AD - Beijing Key Laboratory of Collaborative Innovation in Frontier Technologies for Population Quality, Beijing, China. AD - National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China. AD - National Clinical Key Specialty Construction Program, P.R. China (2023), Beijing, China. FAU - Deng, Feng AU - Deng F AD - State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China. AD - Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China. AD - Beijing Key Laboratory of Collaborative Innovation in Frontier Technologies for Population Quality, Beijing, China. AD - National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China. AD - National Clinical Key Specialty Construction Program, P.R. China (2023), Beijing, China. FAU - Liu, Fenting AU - Liu F AD - State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China. AD - Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China. AD - Beijing Key Laboratory of Collaborative Innovation in Frontier Technologies for Population Quality, Beijing, China. AD - National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China. AD - National Clinical Key Specialty Construction Program, P.R. China (2023), Beijing, China. FAU - Chen, Xiunan AU - Chen X AD - State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China. AD - Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China. AD - Beijing Key Laboratory of Collaborative Innovation in Frontier Technologies for Population Quality, Beijing, China. AD - National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China. AD - National Clinical Key Specialty Construction Program, P.R. China (2023), Beijing, China. FAU - Ning, Ke AU - Ning K AD - State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China. AD - Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China. AD - Beijing Key Laboratory of Collaborative Innovation in Frontier Technologies for Population Quality, Beijing, China. AD - National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China. AD - National Clinical Key Specialty Construction Program, P.R. China (2023), Beijing, China. FAU - Mo, Dan AU - Mo D AD - State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China. AD - Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China. AD - Beijing Key Laboratory of Collaborative Innovation in Frontier Technologies for Population Quality, Beijing, China. AD - National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China. AD - National Clinical Key Specialty Construction Program, P.R. China (2023), Beijing, China. FAU - Zeng, Zhonghong AU - Zeng Z AD - State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China. AD - Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China. AD - Beijing Key Laboratory of Collaborative Innovation in Frontier Technologies for Population Quality, Beijing, China. AD - National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China. AD - National Clinical Key Specialty Construction Program, P.R. China (2023), Beijing, China. FAU - Huang, Ziying AU - Huang Z AD - State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China. AD - Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China. AD - Beijing Key Laboratory of Collaborative Innovation in Frontier Technologies for Population Quality, Beijing, China. AD - National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China. AD - National Clinical Key Specialty Construction Program, P.R. China (2023), Beijing, China. FAU - Dong, Yunshu AU - Dong Y AD - State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China. AD - Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China. AD - Beijing Key Laboratory of Collaborative Innovation in Frontier Technologies for Population Quality, Beijing, China. AD - National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China. AD - National Clinical Key Specialty Construction Program, P.R. China (2023), Beijing, China. FAU - Wang, Yinxue AU - Wang Y AD - State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China. AD - Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China. AD - Beijing Key Laboratory of Collaborative Innovation in Frontier Technologies for Population Quality, Beijing, China. AD - National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China. AD - National Clinical Key Specialty Construction Program, P.R. China (2023), Beijing, China. FAU - Zhang, Chao AU - Zhang C AUID- ORCID: 0000-0001-8019-7998 AD - Department of Medicine, Boston University School of Medicine, Boston, MA, USA. FAU - Qiao, Jie AU - Qiao J AUID- ORCID: 0000-0003-2126-1376 AD - State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China. AD - Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China. AD - Beijing Key Laboratory of Collaborative Innovation in Frontier Technologies for Population Quality, Beijing, China. AD - National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China. AD - National Clinical Key Specialty Construction Program, P.R. China (2023), Beijing, China. FAU - Yu, Yang AU - Yu Y AUID- ORCID: 0000-0002-4310-1966 AD - State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China. yuyang5012@hotmail.com. AD - Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China. yuyang5012@hotmail.com. AD - Beijing Key Laboratory of Collaborative Innovation in Frontier Technologies for Population Quality, Beijing, China. yuyang5012@hotmail.com. AD - National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China. yuyang5012@hotmail.com. AD - National Clinical Key Specialty Construction Program, P.R. China (2023), Beijing, China. yuyang5012@hotmail.com. AD - Clinical Stem Cell Research Center, Peking University Third Hospital, Beijing, China. yuyang5012@hotmail.com. AD - Beijing Advanced Center of Cellular Homeostasis and Aging-Related Diseases, Institute of Advanced Clinical Medicine, Peking University, Beijing, China. yuyang5012@hotmail.com. FAU - Pan, Heng AU - Pan H AUID- ORCID: 0000-0002-5407-5687 AD - State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China. hep2007@bjmu.edu.cn. AD - Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China. hep2007@bjmu.edu.cn. AD - Beijing Key Laboratory of Collaborative Innovation in Frontier Technologies for Population Quality, Beijing, China. hep2007@bjmu.edu.cn. AD - National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China. hep2007@bjmu.edu.cn. AD - National Clinical Key Specialty Construction Program, P.R. China (2023), Beijing, China. hep2007@bjmu.edu.cn. FAU - Li, Rong AU - Li R AUID- ORCID: 0000-0003-0305-5579 AD - State Key Laboratory of Female Fertility Promotion, Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China. roseli001@sina.com. AD - Key Laboratory of Assisted Reproduction (Peking University), Ministry of Education, Beijing, China. roseli001@sina.com. AD - Beijing Key Laboratory of Collaborative Innovation in Frontier Technologies for Population Quality, Beijing, China. roseli001@sina.com. AD - National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China. roseli001@sina.com. AD - National Clinical Key Specialty Construction Program, P.R. China (2023), Beijing, China. roseli001@sina.com. LA - eng GR - 82288102,81925013,82225019,82101714/National Natural Science Foundation of China (National Science Foundation of China)/ PT - Journal Article DEP - 20261028 PL - England TA - Signal Transduct Target Ther JT - Signal transduction and targeted therapy JID - 101676423 RN - EC 2.7.11.1 (TOR Serine-Threonine Kinases) RN - EC 2.7.11.31 (AMP-Activated Protein Kinases) RN - 7413S0WMH6 (ginsenoside Rb1) RN - 0 (Ginsenosides) RN - 0 (NF-kappa B) RN - EC 2.7.1.1 (MTOR protein, human) SB - IM MH - Female MH - *TOR Serine-Threonine Kinases/genetics MH - Animals MH - Humans MH - Cellular Senescence/drug effects/genetics MH - *AMP-Activated Protein Kinases/genetics/metabolism MH - Mice MH - Endometrium/drug effects/pathology/metabolism MH - *Ginsenosides/pharmacology MH - Signal Transduction/drug effects MH - *Aging/drug effects/genetics/pathology MH - NF-kappa B/genetics MH - Stromal Cells/drug effects/pathology/metabolism MH - Oxidative Stress/drug effects MH - *Uterus/drug effects/pathology PMC - PMC13714316 COIS- Competing interests: The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/28 CRDT- 2026/10/28 00:12 PHST- 2025/11/18 00:00 [received] PHST- 2026/07/24 00:00 [accepted] PHST- 2026/03/03 00:00 [revised] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:12 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - 10.1038/s41392-026-02970-4 [pii] AID - 2970 [pii] AID - 10.1038/s41392-026-02970-4 [doi] PST - epublish SO - Signal Transduct Target Ther. 2026 Oct 28;11(1):472. doi: 10.1038/s41392-026-02970-4. PMID- 42946685 OWN - NLM STAT- Publisher LR - 20261028 IS - 1532-0987 (Electronic) IS - 0891-3668 (Linking) DP - 2026 Oct 28 TI - Breaking the Barrier: MBI-LCBI and Recent Beta-lactam Exposure as Predictors of Piperacillin-Tazobactam Resistance in Pediatric Hemato-oncology Patients. LID - 10.1097/INF.0000000000005457 [doi] AB - BACKGROUND: Gram-negative bloodstream infections (BSIs) in pediatric hemato-oncology patients, particularly those with neutropenia, are associated with substantial morbidity and mortality. While piperacillin-tazobactam (PTZ) remains a standard empiric therapy, increasing resistance poses a significant clinical challenge. Identifying risk factors for PTZ-resistant (PTZ-R) BSI is crucial for optimizing empiric antibiotic selection and improving clinical outcomes. METHODS: This retrospective study (2015-2024) analyzed pediatric hemato-oncology Gram-negative BSIs at a northern Israeli tertiary center. Using clinical and microbiologic data, we performed univariate and multivariate logistic regression to identify independent predictors of PTZ resistance. RESULTS: Overall, 284 episodes in 162 patients were evaluated for PTZ susceptibility. Seventy (24.6%) episodes in 56 (34.6%) patients were PTZ-R. Mucosal barrier injury laboratory-confirmed BSI (MBI-LCBI) accounted for 46.1% of episodes, central line-associated bloodstream infection for 50% and secondary BSI for 3.9%. MBI-LCBI (68.6% vs. 38.8%, P < 0.001), beta-lactam use within the preceding 30 days (67.2% vs. 43.2%, P < 0.001), and colonization with extended-spectrum beta-lactamases and carbapenemase-producing Enterobacterales (14.3% vs. 5.6%, P = 0.018) were markedly more common among PTZ-R episodes. Additionally, nonlocal residency (5.7% vs. 16.1%, P = 0.014) and hematologic malignancy (85.5% vs. 62.5%, P = 0.003) were significantly more prevalent in patients with PTZ-R episodes. In multivariate analysis, MBI-LCBI (odds ratio 3.1, 95% confidence interval 1.7-6) and recent beta-lactam exposure (odds ratio 2.2, 95% confidence interval 1.2-4.1) were independent predictors of PTZ-R. Thirty-day mortality occurred in 9 (4.2%) cases; all were PTZ sensitive (P = 0.119). CONCLUSIONS: High PTZ-R in pediatric hemato-oncology BSIs is driven by recent beta-lactam use and MBI-LCBI. Identifying these risk factors may support the selection of appropriate empiric therapy for high-risk patients. CI - Copyright (c) 2026 Wolters Kluwer Health, LLC. All rights reserved. FAU - Nasrallah, Elias AU - Nasrallah E AUID- ORCID: 0000-0002-1865-3283 AD - From the Pediatric Infectious Diseases Unit, Ruth Rappaport Children's Hospital, Haifa, Israel. AD - The Azrieli Faculty of Medicine, Bar-Ilan University, Safed, Israel. FAU - Lubovsky, Dana AU - Lubovsky D AD - The Azrieli Faculty of Medicine, Bar-Ilan University, Safed, Israel. FAU - Hassan, Shadia AU - Hassan S AD - From the Pediatric Infectious Diseases Unit, Ruth Rappaport Children's Hospital, Haifa, Israel. FAU - Szwarcwort, Moran AU - Szwarcwort M AD - Microbiology Laboratory, Rambam Health Care Campus, Haifa, Israel. FAU - Pollak, Dina AU - Pollak D AD - Microbiology Laboratory, Rambam Health Care Campus, Haifa, Israel. FAU - Arad-Cohen, Nira AU - Arad-Cohen N AD - Pediatric Hematology-Oncology Department, Ruth Rappaport Children's Hospital, Haifa, Israel. AD - Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel. FAU - Dabaja-Younis, Halima AU - Dabaja-Younis H AD - Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel. AD - Infection Prevention and Control Unit, Rambam Health Care Campus, Haifa, Israel. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - Pediatr Infect Dis J JT - The Pediatric infectious disease journal JID - 8701858 SB - IM OTO - NOTNLM OT - gram-negative OT - mucosal barrier injury OT - pediatric OT - piperacillin-tazobactam OT - resistance COIS- The authors have no funding or conflicts of interest to disclose. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/28 00:03 PHST- 2026/04/30 00:00 [received] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/28 00:03 [entrez] AID - 00006454-990000000-01913 [pii] AID - 10.1097/INF.0000000000005457 [doi] PST - aheadofprint SO - Pediatr Infect Dis J. 2026 Oct 28. doi: 10.1097/INF.0000000000005457. PMID- 42946678 OWN - NLM STAT- Publisher LR - 20261027 IS - 1945-7197 (Electronic) IS - 0021-972X (Linking) DP - 2026 Oct 28 TI - Would Artificial Intelligence Make 'Good' Decisions About Thyroid Nodule Biopsies? LID - dgag433 [pii] LID - 10.1210/clinem/dgag433 [doi] AB - BACKGROUND: Large language models (LLMs) have shown promise for clinical decision support (CDS), but evaluation under clinical equipoise-scenarios lacking a consensus correct answer-remains rare. We evaluated commercially available LLM performance for thyroid nodule biopsy recommendations under clinical equipoise. METHODS: One hundred clinical vignettes describing patients with incidentally discovered thyroid nodules of varying sizes and risk features were presented to 94 clinicians, recruited by convenience sampling from professional networks, and multiple LLMs (GPT-5, Claude Sonnet 3.5/4.0/4.5, Llama 3.2-90B, Deepseek R1, and OpenEvidence). Responses were dichotomized as biopsy versus no biopsy. Agreement was assessed using Krippendorff's alpha, and a deterministic algorithm served as a provisional benchmark. Selected LLM responses were reviewed by experts. RESULTS: Clinicians recommended biopsy in 58% of responses (95% CI 50%, 66%), with unanimous responses in only 50 vignettes. LLM biopsy rates ranged from 46% (Claude 3.5) to 74% (Llama 3.2-90B). LLM-clinician agreement was moderate (alpha = 0.372-0.453). Agreement with the deterministic algorithm ranged from high (alpha = 0.954, Detailed TIRADS GPT-5) to low (alpha = 0.265, Claude Sonnet 4.5). Llama 3.2-90B recommended biopsy in 8 of 8 cases where clinicians unanimously recommended against it. CONCLUSIONS: LLMs differed meaningfully in biopsy recommendation patterns, with potential population-level consequences. Evaluating and aligning LLMs for CDS under equipoise requires stakeholders and processes analogous to clinical guideline committees. Because LLMs used as CDS could powerfully disseminate different practice patterns, this creates new responsibilities for guidelines committees and other stakeholders. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. See the journal About page for additional terms. FAU - Barage, Abhay AU - Barage A AD - Memorial Sloan Kettering Cancer Center. FAU - Austria, Mia AU - Austria M AD - Memorial Sloan Kettering Cancer Center. FAU - Vertosick, Emily AU - Vertosick E AUID- ORCID: 0000-0001-6948-9850 AD - Memorial Sloan Kettering Cancer Center. FAU - Boucai, Laura AU - Boucai L AUID- ORCID: 0000-0002-8852-1120 AD - Memorial Sloan Kettering Cancer Center. FAU - Farooki, Azeez AU - Farooki A AD - Memorial Sloan Kettering Cancer Center. FAU - Fish, Stephanie AU - Fish S AD - Memorial Sloan Kettering Cancer Center. FAU - Newman, Samantha K AU - Newman SK AD - Memorial Sloan Kettering Cancer Center. FAU - Patrizio, Armando AU - Patrizio A AD - Azienda Ospedaliero Universitaria Pisana. FAU - Presswala, Lubaina AU - Presswala L AD - Memorial Sloan Kettering Cancer Center. FAU - Ryan, Tyler AU - Ryan T AD - Weill Cornell Medicine. FAU - Sabra, Mona AU - Sabra M AUID- ORCID: 0000-0001-8686-2258 AD - Memorial Sloan Kettering Cancer Center. FAU - Zeuren, Rebecca AU - Zeuren R AD - Memorial Sloan Kettering Cancer Center. FAU - Vickers, Andrew AU - Vickers A AD - Memorial Sloan Kettering Cancer Center. FAU - Kuperman, Gilad AU - Kuperman G AD - Columbia University. FAU - Ancker, Jessica S AU - Ancker JS AD - Vanderbilt University. FAU - Kim, Scott Y H AU - Kim SYH AD - National Institutes of Health. FAU - Tuttle, R Michael AU - Tuttle RM AD - Memorial Sloan Kettering Cancer Center. FAU - Flory, James H AU - Flory JH AUID- ORCID: 0000-0002-0259-969X AD - Weill Cornell Medicine. AD - Regeneron Pharmaceuticals. LA - eng PT - Journal Article DEP - 20261028 PL - United States TA - J Clin Endocrinol Metab JT - The Journal of clinical endocrinology and metabolism JID - 0375362 SB - IM OTO - NOTNLM OT - artificial intelligence OT - clinical decision support OT - diagnosis OT - equipoise OT - survey OT - thyroid nodule EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/27 23:53 PHST- 2026/06/23 00:00 [received] PHST- 2026/09/11 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/27 23:53 [entrez] AID - 9003308 [pii] AID - 10.1210/clinem/dgag433 [doi] PST - aheadofprint SO - J Clin Endocrinol Metab. 2026 Oct 28:dgag433. doi: 10.1210/clinem/dgag433. PMID- 42946657 OWN - NLM STAT- Publisher LR - 20261027 IS - 1365-2036 (Electronic) IS - 0269-2813 (Linking) DP - 2026 Oct 27 TI - Management and Outcomes of Bleeding Ectopic Varices in Patients With Liver Cirrhosis: A Systematic Review and Pooled Analysis. LID - 10.1111/apt.71072 [doi] AB - BACKGROUND: Bleeding ectopic varices is a rare but life-threatening complication in patients with clinically significant portal hypertension and liver cirrhosis, with limited evidence available to guide treatment decisions. AIM: To evaluate the safety and efficacy of current therapies for bleeding ectopic varices. METHODS: A systematic review with pooled analysis was performed based on searches conducted in Embase, MEDLINE and Cochrane Library databases. Case series, retrospective and prospective studies including adults with a history of liver cirrhosis or portal hypertension presenting with bleeding ectopic varices were included. Individual patient data on interventions, rebleeding, complications and mortality were pooled and summarised using descriptive analysis. No conventional meta-analysis was conducted due to the heterogeneous nature of included studies. RESULTS: 44 studies (15 non-consecutive case series, 25 single-arm retrospective cohort studies, 3 double-arm retrospective cohort studies and 1 single-arm prospective study) involving 458 patients met selection criteria. Alcohol-related liver disease (38.7%), viral hepatitis (18.8%) and primary sclerosing cholangitis (15.7%) were the most common aetiologies. Child-Pugh A, B and C cirrhosis accounted for 29.7%, 46.7% and 23.5% of patients, respectively, and the pooled median Model for End Stage Liver Disease score was 14.0 (IQR 10.0-20.8). The most reported sites of ectopic varices were duodenal (27.7%), rectal (27.3%) and stomal/peri-stomal (23.1%). Endoscopic sclerosant injection appeared to have a lower rebleeding rate than band ligation in the treatment of bleeding rectal varices (3.3% vs. 33.3%). Across variceal subtypes, transjugular intrahepatic portosystemic shunt (TIPS) combined with variceal embolisation, was observed to have a lower rebleeding rate than TIPS alone. The pooled all-cause mortality following small bowel, rectal and stomal variceal bleeding were 25.0%, 42.9%, and 66.6%, respectively, over a median follow-up period of 354 (IQR 120-780) days. Small sample sizes, missing data and significant heterogeneity between studies limited data quality. CONCLUSION: Bleeding ectopic varices frequently recurs and carries high mortality. An individualised approach in conjunction with early multidisciplinary team discussions is essential in achieving favourable patient outcomes. CI - (c) 2026 John Wiley & Sons Ltd. FAU - Zhu, Angela AU - Zhu A AUID- ORCID: 0009-0008-7817-9545 AD - Department of Gastroenterology and Hepatology, Alfred Health, Melbourne, Victoria, Australia. FAU - Rasaratnam, Kiran AU - Rasaratnam K AUID- ORCID: 0009-0001-6618-9964 AD - Department of Gastroenterology and Hepatology, Alfred Health, Melbourne, Victoria, Australia. FAU - Majeed, Ammar AU - Majeed A AUID- ORCID: 0000-0002-7024-8787 AD - Department of Gastroenterology and Hepatology, Alfred Health, Melbourne, Victoria, Australia. FAU - Kemp, William AU - Kemp W AUID- ORCID: 0000-0002-0787-7273 AD - Department of Gastroenterology and Hepatology, Alfred Health, Melbourne, Victoria, Australia. FAU - Clements, Warren AU - Clements W AUID- ORCID: 0000-0003-1859-5850 AD - School of Translational Medicine, Monash University, Melbourne, Australia. AD - Department of Radiology, Alfred Health, Melbourne, Australia. AD - National Trauma Research Institute, Melbourne, Victoria, Australia. FAU - Roberts, Stuart K AU - Roberts SK AUID- ORCID: 0000-0002-9015-7997 AD - Department of Gastroenterology and Hepatology, Alfred Health, Melbourne, Victoria, Australia. AD - School of Translational Medicine, Monash University, Melbourne, Australia. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - England TA - Aliment Pharmacol Ther JT - Alimentary pharmacology & therapeutics JID - 8707234 SB - IM OTO - NOTNLM OT - cirrhosis OT - ectopic varices OT - portal hypertension EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/27 23:23 PHST- 2026/09/11 00:00 [revised] PHST- 2026/04/28 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/27 23:23 [entrez] AID - 10.1111/apt.71072 [doi] PST - aheadofprint SO - Aliment Pharmacol Ther. 2026 Oct 27. doi: 10.1111/apt.71072. PMID- 42946656 OWN - NLM STAT- Publisher LR - 20261027 IS - 1521-3765 (Electronic) IS - 0947-6539 (Linking) DP - 2026 Oct 27 TI - Deciphering the Access to Fused Oxepinone/Aza-Oxepinone Scaffold Involving Paradoxical O-Nucleophilicity Enabled by Amide-Imide Tautomerization. LID - e71858 [pii] LID - 10.1002/chem.71858 [doi] AB - An atom-economical Rh(III)-catalyzed regioselective one-pot C(sp(2)) horizontal line H activation/[5 + 2] annulation-lactonization sequence has been materialized allowing 3-arylquinoxalin-2(1H)-ones/ 3-arylquinolones/ N-phenylbenzamide (amide/ lactam skeleton) and maleimides to interact, which eventually led to the straightforward access to biologically relevant and structurally unique lactone-fused heterocyclic scaffolds. The transformation proceeds through a cascade sequence involving C(sp(2)) horizontal line H functionalization followed by O-annulation (5 + 2 cyclization) enabled by amide-imide or lactam-lactim tautomerization resulting O-centered nucleophilicity which leads to paradoxical lactonization under redox-neutral condition, averting the demand of any sacrificial oxidant. The methodology exhibits broad substrate scope, excellent functional-group tolerance, and is applicable to both cyclic and acyclic amide substrates, providing easy access to diverse seven-membered fused-lactone frameworks in good to excellent yields. Mechanistic investigations, including control experiments, isotope-labeling studies, and DFT calculations, support the proposed catalytic pathway and provide insight into the observed reactivity and selectivity. CI - (c) 2026 Wiley-VCH GmbH. FAU - Islam, Saiful AU - Islam S AUID- ORCID: 0009-0005-0398-904X AD - Department of Chemistry, University of Calcutta, Kolkata, West Bengal, India. FAU - Das, Dwaipayan AU - Das D AUID- ORCID: 0000-0003-2754-5812 AD - Department of Chemistry, University of Calcutta, Kolkata, West Bengal, India. FAU - Ghosh, Swarnali AU - Ghosh S AD - Department of Chemistry, University of Calcutta, Kolkata, West Bengal, India. FAU - Sarkar, Anindita AU - Sarkar A AUID- ORCID: 0009-0004-0708-8882 AD - Department of Chemistry, University of Calcutta, Kolkata, West Bengal, India. FAU - Mahato, Akhilesh AU - Mahato A AD - Department of Chemistry, Sidho-Kanho-Birsha University, Purulia, West Bengal, India. FAU - Pramanik, Anup AU - Pramanik A AUID- ORCID: 0000-0001-9721-8310 AD - Department of Chemistry, Sidho-Kanho-Birsha University, Purulia, West Bengal, India. FAU - Das, Asish R AU - Das AR AUID- ORCID: 0000-0001-8900-550X AD - Department of Chemistry, University of Calcutta, Kolkata, West Bengal, India. LA - eng GR - University of Calcutta/ GR - 201610088564/UGC-INDIA/ GR - 211610100891/UGC-INDIA/ PT - Journal Article DEP - 20261027 PL - Germany TA - Chemistry JT - Chemistry (Weinheim an der Bergstrasse, Germany) JID - 9513783 SB - IM OTO - NOTNLM OT - C horizontal line H functionalization OT - Rh (III) catalysis OT - [5 + 2] annulation OT - lactam-lactim tautomerization OT - oxepinone/aza-oxepinone skeleton EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/27 23:23 PHST- 2026/10/12 00:00 [revised] PHST- 2026/08/03 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/27 23:23 [entrez] AID - e71858 [pii] AID - 10.1002/chem.71858 [doi] PST - aheadofprint SO - Chemistry. 2026 Oct 27:e71858. doi: 10.1002/chem.71858. PMID- 42946639 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1536-2442 (Electronic) IS - 1536-2442 (Linking) VI - 26 IP - 5 DP - 2026 Sep 1 TI - Molecular and functional characterization of a typical 2-Cys peroxiredoxin and its developmental and pyriproxyfen-responsive expression in Aedes albopictus (Diptera: Culicidae). LID - 10.1093/jisesa/ieag119 [doi] LID - ieag119 AB - The Asian tiger mosquito, Aedes albopictus (Skuse, 1894) (Diptera: Culicidae), is established and expanding across the Black Sea region, but the redox processes associated with its development and response to insect growth regulators remain poorly understood. We characterized a typical 2-Cys peroxiredoxin (Prx2) by examining intraspecific sequence variation, conserved protein features, enzymatic activity, developmental transcription, and transcription following exposure to 0.06 ppb pyriproxyfen. The coding region was polymorphic among Turkish isolates, but all substitutions were synonymous, and the deduced amino acid sequence was conserved. Catalytic cysteines and hallmark motifs supported classification as a typical 2-Cys peroxiredoxin. Recombinant His-tagged Prx2 migrated at the expected molecular size and removed hydrogen peroxide in a concentration- and thiol-dependent manner. Basal transcript abundance varied across development, reaching its lowest level in pupae and higher levels in adults. Exposure to 0.06 ppb pyriproxyfen was associated with increased Prx2 transcript abundance in L3 and L4 larvae, whereas no statistically detectable treatment-related difference was observed among the surviving pupae or adults at the sampled time points. Together, these findings identify Prx2 as a conserved, functional thiol peroxidase and demonstrate a pyriproxyfen-associated larval transcriptional response under the conditions tested. Because this concentration was not independently validated as sublethal in the present colony and later-stage sampling necessarily comprised survivors of the original exposure, the data do not distinguish developmental attenuation of the response from survivor selection. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of Entomological Society of America. FAU - Arslanhan, Batuhan Askim AU - Arslanhan BA AUID- ORCID: 0009-0001-0340-1948 AD - Department of Parasitology, Faculty of Veterinary Medicine, Erciyes University, Melikgazi, Kayseri, Turkiye. FAU - Diop, Samba Deguene AU - Diop SD AD - Department of Parasitology, Faculty of Veterinary Medicine, Erciyes University, Melikgazi, Kayseri, Turkiye. FAU - Sahin, Simge AU - Sahin S AUID- ORCID: 0000-0001-8372-6365 AD - Department of Parasitology, Faculty of Veterinary Medicine, Erciyes University, Melikgazi, Kayseri, Turkiye. FAU - Toroslu, Ahsen Meliha AU - Toroslu AM AD - Department of Parasitology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Atakum, Samsun, Turkiye. FAU - Kizgin, Alina Denis AU - Kizgin AD AUID- ORCID: 0009-0005-5665-8738 AD - Department of Parasitology, Faculty of Veterinary Medicine, Erciyes University, Melikgazi, Kayseri, Turkiye. FAU - Pekmezci, Gokmen Zafer AU - Pekmezci GZ AUID- ORCID: 0000-0002-7791-1959 AD - Department of Preclinical Sciences, Faculty of Veterinary Medicine, Ondokuz Mayis University, Atakum, Samsun, Turkiye. FAU - Inci, Abdullah AU - Inci A AUID- ORCID: 0000-0003-1614-0756 AD - Department of Parasitology, Faculty of Veterinary Medicine, Erciyes University, Melikgazi, Kayseri, Turkiye. FAU - Yildirim, Alparslan AU - Yildirim A AUID- ORCID: 0000-0001-9868-0363 AD - Department of Parasitology, Faculty of Veterinary Medicine, Erciyes University, Melikgazi, Kayseri, Turkiye. LA - eng GR - TSA-2025-15247/Erciyes University Scientific Research Projects Coordination Unit/ PT - Journal Article PL - United States TA - J Insect Sci JT - Journal of insect science (Online) JID - 101096396 RN - 3Q9VOR705O (pyriproxyfen) RN - 0 (Pyridines) RN - EC 1.11.1.15 (Peroxiredoxins) RN - 0 (Insect Proteins) RN - 0 (Juvenile Hormones) RN - Aedes albopictus SB - IM MH - Animals MH - *Aedes/growth & development/genetics/metabolism/drug effects MH - *Pyridines/pharmacology MH - *Peroxiredoxins/metabolism/genetics/chemistry MH - Amino Acid Sequence MH - Larva/growth & development/genetics/metabolism/drug effects MH - Pupa/growth & development/genetics/metabolism/drug effects MH - *Insect Proteins/metabolism/genetics/chemistry MH - *Juvenile Hormones/pharmacology PMC - PMC13713186 OTO - NOTNLM OT - juvenile hormone signaling OT - mosquito physiology OT - oxidative stress OT - thioredoxin system OT - xenobiotic response COIS- None declared. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:29 PMCR- 2026/10/27 CRDT- 2026/10/27 23:13 PHST- 2026/08/23 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/28 05:29 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/27 23:13 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 9003296 [pii] AID - ieag119 [pii] AID - 10.1093/jisesa/ieag119 [doi] PST - ppublish SO - J Insect Sci. 2026 Sep 1;26(5):ieag119. doi: 10.1093/jisesa/ieag119. PMID- 42946638 OWN - NLM STAT- Publisher LR - 20261027 IS - 1525-1438 (Electronic) IS - 1048-891X (Linking) DP - 2026 Sep 29 TI - Single-port robotic intra-peritoneal para-aortic lymphadenectomy: step-by-step technique with the da Vinci SP platform. PG - 105631 LID - S1048-891X(26)01162-X [pii] LID - 10.1016/j.ijgc.2026.105631 [doi] FAU - Rovira, Ramon AU - Rovira R AD - IR SANT PAU, Hospital de la Santa Creu i Sant Pau, Univ Autonoma Barcelona, Barcelona, Spain. Electronic address: ramonroviran@gmail.com. FAU - Bonaldo, Giulio AU - Bonaldo G AD - IR SANT PAU, Hospital de la Santa Creu i Sant Pau, Univ Autonoma Barcelona, Barcelona, Spain; Editorial Board Member, International Journal of Gynecological Cancer. FAU - Magret, Eva AU - Magret E AD - IR SANT PAU, Hospital de la Santa Creu i Sant Pau, Univ Autonoma Barcelona, Barcelona, Spain. FAU - Munoz, Raquel AU - Munoz R AD - IR SANT PAU, Hospital de la Santa Creu i Sant Pau, Univ Autonoma Barcelona, Barcelona, Spain. FAU - Soler, Cristina AU - Soler C AD - IR SANT PAU, Hospital de la Santa Creu i Sant Pau, Univ Autonoma Barcelona, Barcelona, Spain. FAU - Cabrera, Silvia AU - Cabrera S AD - IR SANT PAU, Hospital de la Santa Creu i Sant Pau, Univ Autonoma Barcelona, Barcelona, Spain. LA - eng PT - Journal Article PT - Video-Audio Media DEP - 20260929 PL - United States TA - Int J Gynecol Cancer JT - International journal of gynecological cancer : official journal of the International Gynecological Cancer Society JID - 9111626 SB - IM OTO - NOTNLM OT - Minimally Invasive Surgery OT - Ovarian Cancer OT - Para-Aortic Lymphadenectomy OT - Robotic Assisted Surgery OT - Single Port COIS- Declaration of Competing Interests G.B. was not involved in the editorial review or the decision to publish this article. The other authors declare no conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/27 23:13 PHST- 2026/03/22 00:00 [received] PHST- 2026/08/24 00:00 [revised] PHST- 2026/09/12 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/27 23:13 [entrez] AID - S1048-891X(26)01162-X [pii] AID - 10.1016/j.ijgc.2026.105631 [doi] PST - aheadofprint SO - Int J Gynecol Cancer. 2026 Sep 29:105631. doi: 10.1016/j.ijgc.2026.105631. PMID- 42946635 OWN - NLM STAT- Publisher LR - 20261027 IS - 1532-2831 (Electronic) IS - 1078-8174 (Linking) DP - 2026 Oct 27 TI - Skill mix redesign in Italian radiation oncology: A technical note on advanced practice radiation therapists. PG - 103600 LID - S1078-8174(26)00276-2 [pii] LID - 10.1016/j.radi.2026.103600 [doi] AB - INTRODUCTION: Radiation oncology departments are increasingly required to deliver complex, technology-dependent care while facing medical workforce constraints. In the Italian context, reduced attractiveness of radiation oncology training and increasing workflow complexity highlight the need for transparent skill-mix models. This technical note proposes a practical framework for advanced practice radiation therapists (APRTs) to support selected technical, organisational and protocol-driven components of the radiotherapy pathway while preserving radiation oncologist (RO) accountability for clinical decision-making. METHODS: A structured narrative and implementation-oriented approach was used. Literature on radiotherapy utilisation, workforce constraints, APRT roles, time-driven activity-based costing and governance was integrated with the Italian professional context. Activities were classified according to medical decision intensity, technical standardisation, patient-specific variability, safety risk and suitability for protocol-based execution. RESULTS: Potential APRT roles include simulation preparation, image-guided radiotherapy matching within defined thresholds, organ-at-risk contouring support, auto-contouring review, dosimetric data extraction, toxicity screening, quality assurance, incident reporting and pathway coordination. Non-delegable medical responsibilities include treatment indication, informed consent, prescription, target definition, final plan approval, adaptive decisions affecting prescription or target coverage, toxicity management and oncological follow-up. Safe implementation requires formal education, clinical mentorship, competency assessment, written standard operating procedures, escalation rules, audit and multidisciplinary governance. CONCLUSION: APRTs should not be viewed as substitutes for ROs, but as a structured workforce strategy to preserve medical decision-making capacity and improve workflow resilience in Italian radiotherapy services. IMPLICATIONS FOR PRACTICE: Prospective time-motion, safety and health-economic evaluations are needed to quantify the operational impact and transferability of APRT implementation. CI - Copyright (c) 2026 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Sanna, F AU - Sanna F AD - Radiation Oncology Unit, Azienda Ospedaliero-Universitaria di Sassari, University of Sassari, Sassari, Italy; AOU Azienda Ospedaliera Universitaria di Sassari, Radioterapia Oncologica, Italy. Electronic address: Fsanna3@uniss.it. FAU - Campoccia, S AU - Campoccia S AD - Radiation Oncology Unit, Azienda Ospedaliero-Universitaria di Sassari, University of Sassari, Sassari, Italy; AOU Azienda Ospedaliera Universitaria di Sassari, Radioterapia Oncologica, Italy. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Radiography (Lond) JT - Radiography (London, England : 1995) JID - 9604102 SB - IM OTO - NOTNLM OT - Advanced practice OT - Italy OT - Radiation oncology OT - Radiation therapy OT - Radiotherapy OT - Skill mix OT - Workforce COIS- Conflict of interest statement The authors declare that they have no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/27 23:13 PHST- 2026/06/10 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/27 23:13 [entrez] AID - S1078-8174(26)00276-2 [pii] AID - 10.1016/j.radi.2026.103600 [doi] PST - aheadofprint SO - Radiography (Lond). 2026 Oct 27:103600. doi: 10.1016/j.radi.2026.103600. PMID- 42946619 OWN - NLM STAT- Publisher LR - 20261027 IS - 1440-1592 (Electronic) IS - 1323-8930 (Linking) DP - 2026 Oct 27 TI - Diagnostic performance and clinical utility of open oral food challenges using double-blind, placebo-controlled food challenges as the reference standard: A systematic review. LID - S1323-8930(26)00121-8 [pii] LID - 10.1016/j.alit.2026.09.006 [doi] AB - BACKGROUND: Double-blind, placebo-controlled food challenges (DBPCFCs) are regarded as the reference standard for diagnosing IgE-mediated food allergy owing to their objectivity and ability to minimize bias. However, DBPCFCs are logistically complex, time-consuming, and not always feasible in routine clinical settings. In contrast, open oral food challenges (OFCs) are more accessible and widely used but potentially less reliable due to a lack of blinding. We evaluated the diagnostic performance of open OFCs and descriptively compared their clinical utility. METHODS: We conducted a systematic review of literature available in the Cochrane Central Register of Controlled Trials, PubMed, and Ichushi databases between January 1, 2000, and December 31, 2024, incorporating randomized controlled trials and observational studies in which both open OFCs and DBPCFCs were performed. Risk of bias (RoB) was assessed using QUADAS-2. RESULTS: Eight studies were included. Using DBPCFC as the reference standard, the diagnostic performance of open OFC was as follows: sensitivity, 0.91-0.92 (n = 2); specificity, 0.83-1.00 (n = 3); positive predictive value, 0.00-0.89 (n = 6); and negative predictive value, 0.88-0.97 (n = 2). RoB was high mainly owing to the patient selection and the flow and timing domains. CONCLUSIONS: Given the small number of studies examined, high RoB, and heterogeneity among studies, caution is needed in generalizing these results. Although a negative open OFC result may be reliable, the current evidence is insufficient to establish open OFCs as a complete substitute for DBPCFCs, and large-scale, standardized studies in more diverse populations are needed to clarify their diagnostic performance and clinical utility. CI - Copyright (c) 2026 Japanese Society of Allergology. Published by Elsevier B.V. All rights reserved. FAU - Kuwabara, Yu AU - Kuwabara Y AD - Allergy Center, NHO Mie National Hospital, Mie, Japan. FAU - Kawamoto, Norio AU - Kawamoto N AD - Department of Pediatrics, Graduate School of Medicine, Gifu University, Gifu, Japan. Electronic address: noriok-gif@umin.ac.jp. FAU - Yamamoto, Takeshi AU - Yamamoto T AD - Department of Pediatrics, Graduate School of Medicine, Chiba University, Chiba, Japan. FAU - Arima, Tomoyuki AU - Arima T AD - Allergy Center, NHO Mie National Hospital, Mie, Japan. FAU - Nogami, Kazutaka AU - Nogami K AD - Department of Pediatrics, Sapporo Medical University School of Medicine, Sapporo, Japan. FAU - Suzuki, Shuichi AU - Suzuki S AD - Department of Pediatrics, NHO Shimoshizu National Hospital, Chiba, Japan. FAU - Futamura, Masaki AU - Futamura M AD - Department of Pediatrics, NHO Nagoya Medical Center, Nagoya, Japan. FAU - Yamada, Yoshiyuki AU - Yamada Y AD - Department of Pediatrics, Tokai University School of Medicine, Kanagawa, Japan. FAU - Ebisawa, Motohiro AU - Ebisawa M AD - Department of Pediatric Allergy, Clinical Research Center for Allergy and Rheumatology, NHO Sagamihara National Hospital, Kanagawa, Japan. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Allergol Int JT - Allergology international : official journal of the Japanese Society of Allergology JID - 9616296 SB - IM OTO - NOTNLM OT - Double-blind, placebo-controlled food challenge OT - Food allergy OT - Food hypersensitivity OT - Oral food challenge OT - Systematic review COIS- Conflict of interest COI was disclosed per the policy of the Japanese Society of Pediatric Allergy and Clinical Immunology because this study was conducted in accordance with the Japanese Guidelines for Food Allergy. NK has received research funding from Grants-in-Aid for Scientific Research (KAKENHI) and Nisshin Flour Milling Inc. TY has received research funding from KAKENHI, Japan Agency for Medical Research and Development (AMED), Japan Milk Academic Alliance, and Nipponham Foundation for the Future of Food. TA has received research funding from the Japanese Society of Pediatric Allergy and Clinical Immunology. The rest of the authors have no conflict of interest. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/27 23:12 PHST- 2026/05/11 00:00 [received] PHST- 2026/08/30 00:00 [revised] PHST- 2026/09/14 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/27 23:12 [entrez] AID - S1323-8930(26)00121-8 [pii] AID - 10.1016/j.alit.2026.09.006 [doi] PST - aheadofprint SO - Allergol Int. 2026 Oct 27:S1323-8930(26)00121-8. doi: 10.1016/j.alit.2026.09.006. PMID- 42946615 OWN - NLM STAT- Publisher LR - 20261027 IS - 1578-8989 (Electronic) IS - 0025-7753 (Linking) DP - 2026 Oct 27 TI - Heart failure with preserved ejection fraction: From pathophysiology to a practical approach to diagnosis and treatment. PG - 107617 LID - S0025-7753(26)00268-X [pii] LID - 10.1016/j.medcli.2026.107617 [doi] AB - Heart failure with preserved ejection fraction (HFpEF) is a heterogeneous syndrome with increasing prevalence, driven by aging and cardiometabolic comorbidities. It is better understood as a disorder of elevated filling pressures, impaired cardiovascular reserve, recurrent congestion, and systemic multiorgan dysfunction than as heart failure with normal systolic function. Diagnosis remains probabilistic and requires integration of symptoms, natriuretic peptides, echocardiographic structural and functional abnormalities, and, when resting findings are inconclusive, exercise or invasive hemodynamic testing. Treatment has evolved from symptom control toward phenotype-oriented care. Sodium-glucose cotransporter 2 inhibitors are foundational therapy, finerenone expands options for selected patients, and obesity is now a modifiable therapeutic target. This review summarizes current mechanisms, diagnostic pathways, and treatment strategies, with emphasis on practical clinical decision-making. CI - Copyright (c) 2026 Elsevier Espana, S.L.U. All rights reserved. FAU - Fernandez-Cisnal, Agustin AU - Fernandez-Cisnal A AD - Cardiology Department, Parc Sanitari Sant Joan de Deu, Hospital Sant Boi, Sant Boi de Llobregat, Barcelona, Spain. FAU - Lorenzo, Miguel AU - Lorenzo M AD - Cardiology Department, Hospital Clinico Universitario de Valencia, Valencia, Spain; INCLIVA Biomedical Research Institute, Valencia, Spain. FAU - Palau, Patricia AU - Palau P AD - Cardiology Department, Hospital Clinico Universitario de Valencia, Valencia, Spain; INCLIVA Biomedical Research Institute, Valencia, Spain; Medicine Department, Universitat de Valencia, Valencia, Spain. FAU - Nunez, Julio AU - Nunez J AD - Cardiology Department, Hospital Clinico Universitario de Valencia, Valencia, Spain; INCLIVA Biomedical Research Institute, Valencia, Spain; Medicine Department, Universitat de Valencia, Valencia, Spain; Centro de Investigacion Biomedica en Red - Cardiovascular (CIBER-CV), Madrid, Spain. Electronic address: juenuvi@uv.es. LA - eng LA - spa PT - Journal Article PT - Review DEP - 20261027 PL - Spain TA - Med Clin (Barc) JT - Medicina clinica JID - 0376377 SB - IM OTO - NOTNLM OT - Echocardiography OT - Ecocardiografia OT - Fenotipo OT - Heart failure OT - Inhibidores del cotransportador de sodio-glucosa tipo 2 OT - Insuficiencia cardiaca OT - Natriuretic peptides OT - Phenotype OT - Peptidos natriureticos OT - Sodium-glucose transporter 2 inhibitors OT - Stroke volume OT - Volumen sistolico EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/27 23:12 PHST- 2026/05/20 00:00 [received] PHST- 2026/07/22 00:00 [revised] PHST- 2026/07/23 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/27 23:12 [entrez] AID - S0025-7753(26)00268-X [pii] AID - 10.1016/j.medcli.2026.107617 [doi] PST - aheadofprint SO - Med Clin (Barc). 2026 Oct 27:107617. doi: 10.1016/j.medcli.2026.107617. PMID- 42946581 OWN - NLM STAT- Publisher LR - 20261027 IS - 1879-291X (Electronic) IS - 0301-5629 (Linking) DP - 2026 Oct 28 TI - Therapeutic Ultrasound for Pain Management in Temporomandibular Disorders: A Systematic Review of Clinical Evidence From Controlled and Prospective Studies. LID - S0301-5629(26)00375-3 [pii] LID - 10.1016/j.ultrasmedbio.2026.09.009 [doi] AB - Temporomandibular disorders (TMDs) are a common source of persistent orofacial pain and are estimated to affect a substantial proportion of the population. Therapeutic ultrasound is a non-invasive modality frequently used in musculoskeletal rehabilitation, although its clinical effectiveness for TMD-related pain remains debated. This systematic review was designed and reported in accordance with the PRISMA 2020 recommendations. PubMed/MEDLINE, Scopus, and Web of Science were searched from database inception to April 2026. We included randomized controlled trials, prospective clinical studies, and comparative clinical designs evaluating therapeutic ultrasound for pain management in adults with TMDs. The primary outcomes were pain intensity, assessed using VAS, NRS, or VRS, and mandibular function, assessed mainly by mouth opening. Risk of bias was evaluated using the Cochrane RoB 2.0 tool for randomized trials and ROBINS-I for non-randomized studies. Because of substantial clinical and methodological heterogeneity, findings were synthesized narratively rather than pooled statistically. The search identified 143 records, of which 82 remained after duplicate removal and were screened. Thirty-seven full-text articles were assessed, and 23 studies involving 1256 participants (1226 of them with a temporomandibular disorder) met the eligibility criteria. Twenty-one of 23 studies reported a statistically significant reduction in pain relative to their own baseline; however, these are within-group changes rather than treatment effects, and the certainty of the evidence for pain reduction was very low (GRADE), with low certainty for mandibular function, pressure pain threshold and quality of life/disability. Reported reductions included VAS improvement from 42 to 18 when ultrasound was added to home exercise, compared with 40-26 with exercise alone, and VAS improvement from 4.90 to 1.70 after low-intensity ultrasound therapy in a large randomized trial. Compared with low-level laser therapy or photobiomodulation, four studies favored laser-based treatment, whereas one study favored low-intensity ultrasound combined with exercise. Ultrasound was superior to TENS in two studies, while one study found no meaningful difference among ultrasound, TENS, and LLLT. No consistent difference was observed between continuous and pulsed ultrasound modes. Among randomized trials, 8 studies were judged as low risk of bias, 8 raised some concerns, and 1 was judged as high risk of bias. Adverse events were reported by only 2 of the 23 studies, so the safety of the intervention could not be characterised. Therapeutic ultrasound may reduce pain and improve mandibular function in patients with temporomandibular disorders, particularly as an adjunct to multimodal rehabilitation; however, the certainty of the evidence is very low for pain and low for mandibular function, pressure pain threshold and quality of life/disability, and the optimal treatment parameters remain undetermined. These findings should not be interpreted as establishing clinical efficacy. Although some comparative studies reported greater pain reduction with LLLT/PBM than with ultrasound, this finding should be interpreted cautiously because the number of studies was small and treatment protocols were heterogeneous. Therefore, current evidence does not establish the superiority of LLLT/PBM over therapeutic ultrasound. CI - Copyright (c) 2026 World Federation for Ultrasound in Medicine and Biology. Published by Elsevier Inc. All rights reserved. FAU - Zhou, Xiaofeng AU - Zhou X AD - Department of Pain Medicine, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, 442000, China. FAU - Wang, Dawei AU - Wang D AD - Department of Ultrasound, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, 442000, China. FAU - Jiang, Feng AU - Jiang F AD - Department of Ophthalmology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, 442000, China. FAU - Liu, Hui AU - Liu H AD - Department of Ultrasound, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, 442000, China. Electronic address: lhaaa111111@sina.com. FAU - Wang, Junyan AU - Wang J AD - Department of Radiology, The Sixth Hospital of Wuhan, Affiliated Hospital of Jianghan University, Wuhan, 430000, China. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - England TA - Ultrasound Med Biol JT - Ultrasound in medicine & biology JID - 0410553 SB - IM OTO - NOTNLM OT - Low-intensity pulsed ultrasound OT - Orofacial pain OT - Physical therapy OT - Rehabilitation OT - TMD OT - Temporomandibular disorders OT - Temporomandibular joint OT - Therapeutic ultrasound COIS- Conflict of interest The authors declare no competing interests. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/27 23:11 PHST- 2026/05/28 00:00 [received] PHST- 2026/08/26 00:00 [revised] PHST- 2026/09/08 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/27 23:11 [entrez] AID - S0301-5629(26)00375-3 [pii] AID - 10.1016/j.ultrasmedbio.2026.09.009 [doi] PST - aheadofprint SO - Ultrasound Med Biol. 2026 Oct 28:S0301-5629(26)00375-3. doi: 10.1016/j.ultrasmedbio.2026.09.009. PMID- 42946578 OWN - NLM STAT- Publisher LR - 20261027 IS - 1532-1770 (Electronic) IS - 1521-6942 (Linking) DP - 2026 Oct 28 TI - New developments in the management of osteoarthritis. PG - 102211 LID - S1521-6942(26)00096-3 [pii] LID - 10.1016/j.berh.2026.102211 [doi] AB - Osteoarthritis (OA) is the most common form of arthritis and a leading cause of pain, disability, and healthcare utilization worldwide, responsible for a substantial clinical and socioeconomic burden on society. Currently available and approved therapies remain predominantly symptom-directed, as clinical trials have failed to demonstrate efficacy of any disease-modifying approach that could halt OA progression. However, advances in the understanding of OA pathogenesis have led to the emergence of therapeutic strategies targeting metabolic dysfunction, inflammation, tissue repair, and pain pathways. The present review highlights recent developments in OA management including repurposed metabolic and anti-rheumatic therapies, regenerative approaches such as platelet-rich plasma (PRP) and mesenchymal stem cells, minimally invasive interventional procedures, and emerging biologic and gene therapies. The increased recognition of distinct OA phenotypes has led to the opportunity to translate these discoveries into precision medicine approaches. Still, significant challenges remain in identifying appropriate patient populations and generating the high-quality evidence necessary to bring disease-modifying therapies into clinical practice. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Xia, Boyan AU - Xia B AD - NYU Langone Health, 550 First Avenue, New York, United States. FAU - Grivas, Alexandros AU - Grivas A AD - NYU Langone Health, 550 First Avenue, New York, United States. FAU - Samuels, Jonathan AU - Samuels J AD - NYU Langone Health, 550 First Avenue, New York, United States. Electronic address: jonathan.samuels@nyulangone.org. LA - eng PT - Journal Article PT - Review DEP - 20261028 PL - Netherlands TA - Best Pract Res Clin Rheumatol JT - Best practice & research. Clinical rheumatology JID - 101121149 SB - IM OTO - NOTNLM OT - Disease-modifying drugs OT - GLP-1 receptor agonists OT - Gene therapy OT - Genicular artery embolization OT - Genicular nerve ablation OT - Low dose radiotherapy OT - Mesenchymal stem cells OT - Metformin OT - Osteoarthritis OT - Platelet-rich plasma OT - Precision medicine OT - Regenerative medicine COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/27 23:10 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/27 23:10 [entrez] AID - S1521-6942(26)00096-3 [pii] AID - 10.1016/j.berh.2026.102211 [doi] PST - aheadofprint SO - Best Pract Res Clin Rheumatol. 2026 Oct 28:102211. doi: 10.1016/j.berh.2026.102211. PMID- 42946577 OWN - NLM STAT- Publisher LR - 20261027 IS - 1532-8686 (Electronic) IS - 0037-1963 (Linking) DP - 2026 Oct 1 TI - A small modification makes a big difference: rRNA modifications in hematopoiesis and leukemia. LID - S0037-1963(26)00095-8 [pii] LID - 10.1053/j.seminhematol.2026.09.008 [doi] AB - Hematopoietic stem cells (HSCs) sustain lifelong blood production through a fine balance between self-renewal and differentiation that demands a tight regulation of protein synthesis. HSCs are highly sensitive to translational perturbation, as exemplified by human ribosomopathies-a group of congenital bone marrow failure syndromes caused by mutations in ribosomal components and biogenesis factors. While the contribution of ribosome numbers to hematologic disease is established, ribosome composition is emerging as an equally important axis of regulation. A central source of this compositional diversity is ribosomal RNA (rRNA) nucleotide modifications-human rRNAs carry over 200 chemical modifications, primarily 2'-O-methylation and pseudouridylation, which were long regarded as static structural features but are now recognized as dynamically regulated during cellular differentiation. Perturbation of these modification patterns has profound consequences. In dyskeratosis congenita, germline mutations to rRNA modification-related proteins disrupt rRNA modifications landscape, and cause bone marrow failure through selective translational defects without globally compromising ribosomes numbers. Conversely, in acute myeloid leukemia, leukemic stem cells actively maintain high rRNA modification levels at specific sites to sustain self-renewal, metabolic fitness, and chemoresistance, effectively driving a stem-like translational state. In this review, we integrate findings from normal hematopoietic differentiation, congenital bone marrow failure syndromes, and leukemia to describe how rRNA modifications link translation to cell fate in the hematopoietic system. We discuss the dynamics of rRNA modifications as determinants of cellular identity and highlight emerging therapeutic opportunities, as well as the significant gaps that remain in this expanding field. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Ifergan, Neta AU - Ifergan N AD - Department of Genetics, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel. FAU - Ben Dror, Sivan AU - Ben Dror S AD - Department of Genetics, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel. FAU - Nachmani, Daphna AU - Nachmani D AD - Department of Genetics, Alexander Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, Israel. Electronic address: daphna.nachmani@mail.huji.ac.il. LA - eng PT - Journal Article DEP - 20261001 PL - United States TA - Semin Hematol JT - Seminars in hematology JID - 0404514 SB - IM OTO - NOTNLM OT - Hematopoiesis OT - Hematopoietic stem cells OT - Leukemia OT - Ribosomes OT - rRNA modifications COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/27 23:10 PHST- 2026/06/08 00:00 [received] PHST- 2026/09/07 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/27 23:10 [entrez] AID - S0037-1963(26)00095-8 [pii] AID - 10.1053/j.seminhematol.2026.09.008 [doi] PST - aheadofprint SO - Semin Hematol. 2026 Oct 1:S0037-1963(26)00095-8. doi: 10.1053/j.seminhematol.2026.09.008. PMID- 42946553 OWN - NLM STAT- Publisher LR - 20261027 IS - 1099-1573 (Electronic) IS - 0951-418X (Linking) DP - 2026 Oct 27 TI - Dihydrotanshinone I Targets MIF to Inhibit GSK-3beta/beta-Catenin Axis-Mediated Invasion and Metastasis in Gastric Cancer. LID - 10.1002/ptr.70486 [doi] AB - Salvia miltiorrhiza Bunge has been a commonly used medicinal herb in China since ancient times, widely employed to treat blood stasis-related disorders, including cardiovascular and inflammatory diseases. Dihydrotanshinone I (DHT), a lipophilic diterpenoid derived from its roots, has demonstrated anti-tumor potential; however, its specific molecular mechanisms involved in gastric cancer (GC) metastasis remain a mystery. Here, we report that DHT significantly suppresses the invasion and metastasis of GC by directly targeting macrophage migration inhibitory factor (MIF). Using limited proteolysis-mass spectrometry (LiP-MS), we identified MIF as a direct binding target of DHT, which was further validated by surface plasmon resonance and molecular docking. Mechanistically, DHT binding to MIF restored GSK-3beta activity, promoted beta-catenin phosphorylation (Ser33/37/Thr41), and facilitated its proteasomal degradation, thereby inhibiting beta-catenin nuclear translocation and transcription of epithelial-mesenchymal transition-related genes. In vivo experiments confirmed that DHT markedly suppressed peritoneal metastasis of GC. These findings not only elucidate a novel molecular axis-DHT/MIF/GSK-3beta/beta-catenin-in suppressing GC metastasis but also provide a mechanistic basis for the ethnopharmacological application of Danshen in cancer-related disorders. Furthermore, our findings raise the hypothesis that MIF might serve as a potential predictive biomarker for DHT treatment response in GC, a concept that warrants future clinical validation. CI - (c) 2026 The Author(s). Phytotherapy Research published by John Wiley & Sons Ltd. FAU - Wang, Chuhang AU - Wang C AUID- ORCID: 0009-0006-9174-7602 AD - School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. AD - Zhejiang Key Laboratory of Blood-Stasis-Toxin Syndrome, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. FAU - Ni, Xiaochen AU - Ni X AUID- ORCID: 0000-0003-4759-0709 AD - School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. AD - Zhejiang Key Laboratory of Blood-Stasis-Toxin Syndrome, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. FAU - Pan, Yuyan AU - Pan Y AD - School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. AD - Zhejiang Key Laboratory of Blood-Stasis-Toxin Syndrome, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. AD - The First School of Clinical Medicine, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. FAU - Fan, Wei AU - Fan W AD - School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. AD - Zhejiang Key Laboratory of Blood-Stasis-Toxin Syndrome, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. FAU - Guo, Jianing AU - Guo J AD - School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. AD - Zhejiang Key Laboratory of Blood-Stasis-Toxin Syndrome, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. FAU - Zhang, Kaiyuan AU - Zhang K AUID- ORCID: 0000-0002-0339-010X AD - School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. AD - Zhejiang Key Laboratory of Blood-Stasis-Toxin Syndrome, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. FAU - Zhang, Mingsi AU - Zhang M AD - School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. AD - Zhejiang Key Laboratory of Blood-Stasis-Toxin Syndrome, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. FAU - Zheng, Linfeng AU - Zheng L AD - School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. AD - Zhejiang Key Laboratory of Blood-Stasis-Toxin Syndrome, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. AD - Department of Pathology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang, China. FAU - Wei, Yunhai AU - Wei Y AUID- ORCID: 0000-0002-6647-8985 AD - Huzhou Central Hospital, Huzhou, Zhejiang, People's Republic of China. FAU - Jiang, Tao AU - Jiang T AD - School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. AD - Zhejiang Key Laboratory of Blood-Stasis-Toxin Syndrome, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. AD - Traditional Chinese Medicine "Preventing Disease" Wisdom Health Project Research Center of Zhejiang, Hangzhou, Zhejiang, People's Republic of China. FAU - Zhang, Guangji AU - Zhang G AUID- ORCID: 0000-0003-4525-9422 AD - School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. AD - Zhejiang Key Laboratory of Blood-Stasis-Toxin Syndrome, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, People's Republic of China. AD - Traditional Chinese Medicine "Preventing Disease" Wisdom Health Project Research Center of Zhejiang, Hangzhou, Zhejiang, People's Republic of China. LA - eng GR - 82030119/the National Natural Science Foundation of China (Key Program)/ GR - U23A20499/National Natural Science Foundation of China (Regional Innovation and Development Joint Fund)/ GR - LQ23H270013/the Zhejiang Provincial Natural Science Foundation of China (Youth Program)/ GR - GZY-ZJ-KJ-23094/the Zhejiang Provincial Administration of Traditional Chinese Medicine (Key Research Project of Joint Science and Technology Plan)/ PT - Journal Article DEP - 20261027 PL - England TA - Phytother Res JT - Phytotherapy research : PTR JID - 8904486 SB - IM OTO - NOTNLM OT - Top/Fopflash OT - dihydrotanshinone I OT - gastric cancer OT - macrophage migration inhibitory factor OT - surface plasmon resonance EDAT- 2026/10/28 05:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/27 23:03 PHST- 2026/09/28 00:00 [revised] PHST- 2026/01/16 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/28 05:28 [pubmed] PHST- 2026/10/27 23:03 [entrez] AID - 10.1002/ptr.70486 [doi] PST - aheadofprint SO - Phytother Res. 2026 Oct 27. doi: 10.1002/ptr.70486. PMID- 42946016 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1365-2052 (Electronic) IS - 0268-9146 (Print) IS - 0268-9146 (Linking) VI - 57 IP - 6 DP - 2026 Dec TI - Generative Adversarial Networks for Omics Data: An Overview and Future Directions for Livestock Applications. LID - e70226 [pii] LID - 10.1002/age.70226 [doi] AB - Generative adversarial networks (GANs) are artificial intelligence models designed to generate novel data that is indistinguishable from real data. Originally developed to generate images to complement existing datasets and create more diverse samples, GAN-based data can enhance downstream analysis for machine learning and deep learning applications. GAN-based approaches are also versatile and suitable for augmenting omics data, improving downstream analyses such as genotype prediction, biomarker detection, and protein de novo design. In addition, GANs can preserve and reconstruct genomic regions under selective pressure, increase sample sizes from underrepresented groups, and mitigate class imbalance effects in genomic analyses, transcriptomics, and single-cell omics. Recent work also demonstrates that GAN-generated data can support privacy-preserving data sharing and the design of novel biological sequences and proteins. This literature review presents the foundational concepts of GANs, explores diverse applications across omics fields, and highlights novel opportunities for applying GAN approaches in livestock genomics. CI - (c) 2026 The Author(s). Animal Genetics published by John Wiley & Sons Ltd on behalf of Stichting International Foundation for Animal Genetics. FAU - Vargas, Edgar Caballero AU - Vargas EC AUID- ORCID: 0009-0009-5152-9899 AD - Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. FAU - Bresolin, Tiago AU - Bresolin T AUID- ORCID: 0000-0002-3196-5150 AD - Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. AD - Center for Digital Agriculture, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA. LA - eng PT - Journal Article PT - Review PL - England TA - Anim Genet JT - Animal genetics JID - 8605704 SB - IM MH - Animals MH - *Generative Adversarial Networks MH - *Genomics/methods MH - *Livestock/genetics MH - Generative Artificial Intelligence PMC - PMC13713198 OTO - NOTNLM OT - data augmentation OT - generative models OT - genomics OT - synthetic data COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 04:34 MHDA- 2026/10/28 04:35 PMCR- 2026/10/27 CRDT- 2026/10/27 20:23 PHST- 2026/09/23 00:00 [revised] PHST- 2026/06/09 00:00 [received] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/28 04:35 [medline] PHST- 2026/10/28 04:34 [pubmed] PHST- 2026/10/27 20:23 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e70226 [pii] AID - AGE70226 [pii] AID - 10.1002/age.70226 [doi] PST - ppublish SO - Anim Genet. 2026 Dec;57(6):e70226. doi: 10.1002/age.70226. PMID- 42946015 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1554-7531 (Electronic) IS - 1061-4303 (Print) IS - 1061-4303 (Linking) VI - 98 IP - 11 DP - 2026 Nov TI - Sustainable Pb (II) Removal via Pine-Bark Biochar Valorization: Removal Mechanisms and Response-Surface Modeling. LID - e70640 [pii] LID - 10.1002/wer.70640 [doi] AB - Lead (Pb (II)) is a persistent contaminant in industrial wastewater. This study evaluated pine bark (PB)-derived biochars produced at different pyrolysis temperatures for Pb (II) removal. Biochar produced at 500 degrees C (PB5) achieved the highest removal efficiency and was selected for subsequent experiments. The adsorption equilibrium for PB5 was best described using the Sips isotherm, indicating energetic heterogeneity, whereas its kinetics followed the pseudo-second-order model. The maximum monolayer adsorption capacity estimated using the Langmuir model was 29.754 mg/g, with combined spectroscopic, elemental, and textural analyses suggesting that Pb-containing surface precipitation and pore blocking/filling played major roles in Pb removal. Response surface methodology was used to optimize the pH, adsorbent dosage, temperature, and ionic strength, with the PB5 dosage found to have the strongest influence on adsorption, followed by the pH, ionic strength, and temperature. Among the investigated experimental conditions, the highest removal efficiency of 91.43% was achieved at pH 7, an adsorbent dosage of 5 g/L, a temperature of 25 degrees C, and an ionic strength of 5. The developed RSM model showed satisfactory agreement with the experimental data, with a root mean square error (RMSE) of 3.75 percentage points. CI - (c) 2026 The Author(s). Water Environment Research published by Wiley Periodicals LLC on behalf of Water Environment Federation. FAU - Im, Eun-Chae AU - Im EC AD - Department of Environmental Engineering, Ajou University, Suwon, Republic of Korea. FAU - Lee, Jae-In AU - Lee JI AD - Institute of Agricultural Environmental Science, Hankyong National University, Anseong, Republic of Korea. FAU - Park, Seong-Jik AU - Park SJ AUID- ORCID: 0000-0003-2122-5498 AD - Institute of Agricultural Environmental Science, Hankyong National University, Anseong, Republic of Korea. AD - Department of Bioresources and Rural System Engineering, Hankyong National University, Anseong, Republic of Korea. FAU - Lee, Chang-Gu AU - Lee CG AUID- ORCID: 0000-0002-2304-8635 AD - Department of Environmental Engineering, Ajou University, Suwon, Republic of Korea. AD - Department of Energy Systems Research, Ajou University, Suwon, Republic of Korea. LA - eng GR - RS-2024-00400922/Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries/ PT - Journal Article PL - United States TA - Water Environ Res JT - Water environment research : a research publication of the Water Environment Federation JID - 9886167 RN - 16291-96-6 (Charcoal) RN - 2P299V784P (Lead) RN - 0 (biochar) RN - 0 (Water Pollutants, Chemical) SB - IM MH - *Charcoal/chemistry MH - *Lead/chemistry/isolation & purification MH - *Pinus/chemistry MH - Adsorption MH - *Plant Bark/chemistry MH - *Water Pollutants, Chemical/chemistry/isolation & purification MH - Kinetics MH - Hydrogen-Ion Concentration MH - Temperature MH - Osmolar Concentration PMC - PMC13713201 OTO - NOTNLM OT - Pb (II) adsorption OT - biochar OT - pore filling OT - precipitation OT - waste resource utilization COIS- The authors declare no conflicts of interest. EDAT- 2026/10/28 04:34 MHDA- 2026/10/28 04:35 PMCR- 2026/10/27 CRDT- 2026/10/27 20:23 PHST- 2026/09/17 00:00 [revised] PHST- 2026/07/27 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/28 04:35 [medline] PHST- 2026/10/28 04:34 [pubmed] PHST- 2026/10/27 20:23 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e70640 [pii] AID - WER70640 [pii] AID - 10.1002/wer.70640 [doi] PST - ppublish SO - Water Environ Res. 2026 Nov;98(11):e70640. doi: 10.1002/wer.70640. PMID- 42946552 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1365-2990 (Electronic) IS - 0305-1846 (Print) IS - 0305-1846 (Linking) VI - 52 IP - 6 DP - 2026 Dec TI - Hypothalamic Neurochemical and Microglial Suppression in Long-Standing Human Hibernation-Like Syndrome. LID - e70104 [pii] LID - 10.1111/nan.70104 [doi] AB - Seasonally recurring hibernation-like states are exceptionally rare in humans, and their neural substrates remain unknown. We investigated the brain of a 57-year-old woman with a > 30-year history of winter-associated hypothermia, neuroendocrine instability, psychomotor suppression and circannual rhythmicity. Postmortem neuropathological analysis revealed preserved hypothalamic and basal forebrain anatomy but profound neurochemical abnormalities. Key peptidergic neuronal populations involved in circadian and neuroendocrine regulation, including arginine vasopressin (AVP), oxytocin (OXT), corticotropin-releasing hormone (CRH) and thyrotropin-releasing hormone (TRH) neurons, showed near-complete loss of immunoreactivity in the SCN, SON and PVN. Immunoreactivity for vasoactive intestinal peptide (VIP) and neurotensin (NT) in the SCN was markedly reduced. These changes were accompanied by markedly reduced synaptophysin immunoreactivity in all hypothalamic regions examined, whereas catecholaminergic neurons were relatively preserved in the PVN and SON. Beyond the hypothalamus, cholinergic neurons in the NBM showed a moderate reduction without evidence of Alzheimer-related pathology. Microglial immunoreactivity was nearly absent in the hypothalamic and extrahypothalamic regions examined, contrasting with preserved GFAP and AQP4 immunoreactivity. Moreover, myelination and microvascular architecture also remained intact. These findings identify a selective hypothalamic and microglial suppression associated with a rare human hibernation-like phenotype and indicate markedly reduced output of core neuroendocrine and circadian circuits during long-standing hypometabolic states. The case also illustrates that recurrent, profound hypothermia can be tolerated over decades without systemic organ failure, highlighting an unexpected degree of human metabolic resilience. CI - (c) 2026 The Author(s). Neuropathology and Applied Neurobiology published by John Wiley & Sons Ltd on behalf of British Neuropathological Society. FAU - Jiang, Wei AU - Jiang W AUID- ORCID: 0009-0007-5054-1306 AD - Department of Endocrinology and Metabolism, Amsterdam University Medical Center, Location AMC. University of Amsterdam, Amsterdam, the Netherlands. AD - Netherlands Institute for Neuroscience, Amsterdam, the Netherlands. FAU - Kalsbeek, Andries AU - Kalsbeek A AUID- ORCID: 0000-0001-9606-8453 AD - Department of Endocrinology and Metabolism, Amsterdam University Medical Center, Location AMC. University of Amsterdam, Amsterdam, the Netherlands. AD - Netherlands Institute for Neuroscience, Amsterdam, the Netherlands. FAU - Hut, Roelof A AU - Hut RA AUID- ORCID: 0000-0003-4552-0985 AD - Groningen Institute for Evolutionary Life Sciences, University of Groningen, Groningen, the Netherlands. FAU - Swaab, Dick F AU - Swaab DF AUID- ORCID: 0000-0002-9665-7845 AD - Netherlands Institute for Neuroscience, Amsterdam, the Netherlands. FAU - Yi, Chun-Xia AU - Yi CX AUID- ORCID: 0000-0003-1184-4615 AD - Department of Endocrinology and Metabolism, Amsterdam University Medical Center, Location AMC. University of Amsterdam, Amsterdam, the Netherlands. AD - Netherlands Institute for Neuroscience, Amsterdam, the Netherlands. LA - eng PT - Case Reports PT - Journal Article PL - England TA - Neuropathol Appl Neurobiol JT - Neuropathology and applied neurobiology JID - 7609829 SB - IM MH - Humans MH - Female MH - Middle Aged MH - *Microglia/pathology/metabolism MH - *Hypothalamus/metabolism/pathology MH - *Hibernation/physiology MH - Neurons/metabolism/pathology MH - *Hypothermia/pathology/metabolism PMC - PMC13713431 OTO - NOTNLM OT - glymphatic system OT - hypothalamus OT - microglia OT - nucleus basalis of Meynert OT - thermoregulation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 23:28 MHDA- 2026/10/28 05:28 PMCR- 2026/10/28 CRDT- 2026/10/27 22:54 PHST- 2026/09/23 00:00 [revised] PHST- 2026/04/28 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 22:54 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e70104 [pii] AID - NAN70104 [pii] AID - 10.1111/nan.70104 [doi] PST - ppublish SO - Neuropathol Appl Neurobiol. 2026 Dec;52(6):e70104. doi: 10.1111/nan.70104. PMID- 42946547 OWN - NLM STAT- Publisher LR - 20261027 IS - 2162-1918 (Print) IS - 2162-1918 (Linking) DP - 2026 Oct 27 TI - Chronic Wounds as Biofilm Ecosystems: Modernizing Microbiology Beyond Planktonic Paradigms. PG - 21621918261501846 LID - 10.1177/21621918261501846 [doi] AB - SIGNIFICANCE: Chronic wound infections, including diabetic foot infections, are increasingly recognized as biofilm-associated polymicrobial ecosystems rather than simple planktonic infections. However, routine microbiological workflows remain largely optimized for acute infections, creating a mismatch between laboratory diagnostics and the biological organization of chronic infection. RECENT ADVANCES: Growing evidence from biofilm biology, microbial ecology, and molecular microbiology has demonstrated that chronic wound infections are characterized by structured microbial communities, physiological heterogeneity, and enhanced antimicrobial tolerance. Advanced diagnostic approaches have improved understanding of microbial diversity and biofilm-associated persistence beyond what is captured by conventional culture alone. CRITICAL ISSUES: Conventional microbiological workflows may incompletely resolve the microbial populations most relevant to chronicity, recurrence, and treatment failure. Key limitations include sampling strategy, tissue processing, incubation conditions, anaerobic recovery, and reliance on planktonic susceptibility testing. The discrepancy between minimum inhibitory concentration-based testing and biofilm-associated antimicrobial tolerance highlights an important gap between laboratory assessment and infection biology. FUTURE DIRECTIONS: Modernization of chronic wound microbiology requires a transition from pathogen-centered planktonic models toward biofilm-aware, ecosystem-oriented approaches. Integration of optimized sampling, culture-based diagnostics, selective molecular tools, surgical source control, and antimicrobial stewardship may improve diagnostic resolution, support more rational antimicrobial use, and enhance long-term clinical outcomes.[Figure: see text]. FAU - Drago, Lorenzo AU - Drago L AUID- ORCID: 0000-0002-5206-540X AD - UOC Laboratory of Clinical Medicine with Specialized Areas, IRCCS MultiMedica, Milan, Italy. AD - Department of Biomedical Sciences for Health, Clinical Microbiology and Microbiome Laboratory, University of Milan, Milan, Italy. FAU - De La Motte, Luigi Regenburgh AU - De La Motte LR AUID- ORCID: 0000-0002-8760-1292 AD - UOC Laboratory of Clinical Medicine with Specialized Areas, IRCCS MultiMedica, Milan, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - United States TA - Adv Wound Care (New Rochelle) JT - Advances in wound care JID - 101590593 SB - IM OTO - NOTNLM OT - antimicrobial stewardship OT - biofilms OT - chronic wound infections OT - diabetic foot infections OT - microbiological diagnosis EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 22:43 PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 22:43 [entrez] AID - 10.1177/21621918261501846 [doi] PST - aheadofprint SO - Adv Wound Care (New Rochelle). 2026 Oct 27:21621918261501846. doi: 10.1177/21621918261501846. PMID- 42946543 OWN - NLM STAT- Publisher LR - 20261027 IS - 1557-9034 (Electronic) IS - 1092-6429 (Linking) DP - 2026 Oct 27 TI - Implementing Therapeutic Endoscopic Retrograde Cholangiopancreatography in a Resource-Limited Setting: Lessons from the First Completely Minimally Invasive Management of Choledocholithiasis in Benin Republic. PG - 10926429261502081 LID - 10.1177/10926429261502081 [doi] AB - BACKGROUND: Laparoscopic cholecystectomy combined with therapeutic endoscopic retrograde cholangiopancreatography (ERCP) is the reference minimally invasive treatment for common bile duct stones, but access remains limited in resource-constrained settings. We report the first completely minimally invasive management of choledocholithiasis achieved in Benin. CASE REPORT: A 35-year-old man was referred for acute calculous cholecystitis with 3 months of recurrent right upper quadrant pain, fever, intermittent jaundice, dark urine, and pale stools. Investigation showed hyperbilirubinemia, cholestasis, modest cytolysis, and a coagulopathy corrected by parenteral vitamin K1. Ultrasonography showed multiple gallbladder calculi without a visible ductal stone. Laparoscopic cholecystectomy with transcystic drainage was performed; cholangiography through the drain on postoperative day 6 demonstrated three retained common bile duct stones. A laparoendoscopic rendezvous was planned, but the guidewire could not be advanced antegradely through the drain, and transpapillary salvage was undertaken. Guidewire-assisted cannulation, sphincterotomy, five basket passes, and balloon sweeping achieved complete clearance, confirmed by completion cholangiography. The procedure lasted 260 minutes. No adverse event occurred, and the patient remained asymptomatic at 8 years. TECHNICAL CONSIDERATIONS: Conservative case selection, correction of the coagulopathy before sphincterotomy, rehearsed multidisciplinary preparation, and predefined electrosurgical settings determined the safety of this inaugural procedure. Failure of the rendezvous was anticipated, and a transpapillary fallback was prepared in advance. CONCLUSION: Therapeutic ERCP can complete a fully minimally invasive biliary pathway in a resource-limited setting when introduced through careful case selection, appropriate training, multidisciplinary preparation, and standardized technique. The experience may inform centers developing advanced therapeutic endoscopy in similar settings. FAU - Lawani, Ismail AU - Lawani I AUID- ORCID: 0000-0001-5022-6313 AD - Department of Surgery and Surgical Specialties, Visceral Surgery Unit, Faculty of Health Sciences, University of Abomey-Calavi, Cotonou, Benin. AD - International Hospital of Calavi (CHIC), Abomey-Calavi, Benin. FAU - Kpadonou Chobli, Herve AU - Kpadonou Chobli H AD - Emergency Department, National and University Hospital Hubert Koutoukou Maga, Cotonou, Benin. FAU - Gayito Adagba, Rene Ayaovi AU - Gayito Adagba RA AUID- ORCID: 0000-0003-4361-3993 AD - Department of Surgery and Surgical Specialties, Visceral Surgery Unit, Faculty of Health Sciences, University of Abomey-Calavi, Cotonou, Benin. AD - International Hospital of Calavi (CHIC), Abomey-Calavi, Benin. FAU - Sokpon, Comlan N'dehougbea Martin AU - Sokpon CNM AD - International Hospital of Calavi (CHIC), Abomey-Calavi, Benin. AD - Department of Medicine and Medical Specialties, Hepato-Gastroenterology Unit, Faculty of Health Sciences, University of Abomey-Calavi, Cotonou, Benin. FAU - Imorou Souaibou, Yacoubou AU - Imorou Souaibou Y AD - Department of Surgery and Surgical Specialties, Visceral Surgery Unit, Faculty of Health Sciences, University of Abomey-Calavi, Cotonou, Benin. FAU - Affanou, Mirabelle AU - Affanou M AD - Clinique Medico-Chirurgicale Sainte Anne d'Afrique, Cotonou, Benin. FAU - Gnanhoui, Franck AU - Gnanhoui F AD - Clinique Medico-Chirurgicale Sainte Anne d'Afrique, Cotonou, Benin. FAU - Kpossou, Aboudou Raimi AU - Kpossou AR AD - International Hospital of Calavi (CHIC), Abomey-Calavi, Benin. AD - Department of Medicine and Medical Specialties, Hepato-Gastroenterology Unit, Faculty of Health Sciences, University of Abomey-Calavi, Cotonou, Benin. FAU - Olory-Togbe, Jean Leon AU - Olory-Togbe JL AD - Department of Surgery and Surgical Specialties, Visceral Surgery Unit, Faculty of Health Sciences, University of Abomey-Calavi, Cotonou, Benin. AD - Clinique Medico-Chirurgicale Sainte Anne d'Afrique, Cotonou, Benin. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Laparoendosc Adv Surg Tech A JT - Journal of laparoendoscopic & advanced surgical techniques. Part A JID - 9706293 SB - IM OTO - NOTNLM OT - Benin Republic OT - choledocholithiasis OT - endoscopic retrograde cholangiopancreatography OT - endoscopic sphincterotomy OT - laparoscopic cholecystectomy OT - minimally invasive surgical procedures EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 22:42 PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 22:42 [entrez] AID - 10.1177/10926429261502081 [doi] PST - aheadofprint SO - J Laparoendosc Adv Surg Tech A. 2026 Oct 27:10926429261502081. doi: 10.1177/10926429261502081. PMID- 42946542 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1600-0463 (Electronic) IS - 0903-4641 (Linking) VI - 134 IP - 11 DP - 2026 Nov TI - Stable Expression of Human Endogenous Retrovirus Families and Epigenetic Repressors in Human Granulosa Cells Across Oocyte Maturation Stages. LID - e70276 [pii] LID - 10.1111/apm.70276 [doi] AB - Human endogenous retroviruses (HERVs) comprise a substantial portion of the human genome and are increasingly recognized as regulators of developmental and tissue-specific transcription. While their expression has been widely studied in early embryos and pluripotent stem cells, little is known about their regulation within the ovarian follicular environment. This study aimed to characterize selected HERV families and their main epigenetic repressors in human granulosa cells associated with oocytes at different maturation stages. Expression of HERV-H, HERV-K, and HERV-W families was analyzed, focusing on pol genes, the HERV-W env gene, and the envelope-derived proteins Syncytin-1 and Syncytin-2. The epigenetic repressors TRIM28 and SETDB1 were also evaluated. Samples were stratified into mature metaphase II (M2) and immature metaphase I-germinal vesicle (M1-GV) groups. All targets were consistently expressed. Quantitative analyses showed comparable transcriptional levels between M2- and M1-GV-associated samples, with no significant differences. In an exploratory analysis restricted to M2 oocytes, lower HERV-H expression was nominally associated with successful fertilization (unadjusted p = 0.0292), requiring independent confirmation. HERV transcription and its silencing machinery are stably maintained in granulosa cells across oocyte maturation stages, suggesting a tightly regulated transcriptional equilibrium within the follicular microenvironment. CI - (c) 2026 APMIS - Journal of Pathology, Microbiology and Immunology. FAU - Galliano, Ilaria AU - Galliano I AUID- ORCID: 0000-0002-1273-3178 AD - Department of Public Health and Pediatric Sciences, University of Turin, Turin, Italy. AD - Pediatric Laboratory, Department of Children's Pathology and Care, Regina Margherita Children's Hospital, Turin, Italy. FAU - Paschero, Carlotta AU - Paschero C AD - Gynecology and Obstetrics 1U, Physiopathology of Reproduction and IVF Unit, Department of Surgical Sciences, S. Anna Hospital, University of Turin, Turin, Italy. FAU - Filippo, Valeria Di AU - Filippo VD AD - Department of Public Health and Pediatric Sciences, University of Turin, Turin, Italy. FAU - Proi, Alessandro AU - Proi A AD - Department of Public Health and Pediatric Sciences, University of Turin, Turin, Italy. FAU - Calvi, Cristina AU - Calvi C AD - Department of Public Health and Pediatric Sciences, University of Turin, Turin, Italy. AD - Pediatric Laboratory, Department of Children's Pathology and Care, Regina Margherita Children's Hospital, Turin, Italy. FAU - Montanari, Paola AU - Montanari P AD - Department of Public Health and Pediatric Sciences, University of Turin, Turin, Italy. AD - Pediatric Laboratory, Department of Children's Pathology and Care, Regina Margherita Children's Hospital, Turin, Italy. FAU - Gambarino, Stefano AU - Gambarino S AUID- ORCID: 0009-0005-0321-0112 AD - Department of Public Health and Pediatric Sciences, University of Turin, Turin, Italy. FAU - Revelli, Alberto AU - Revelli A AUID- ORCID: 0000-0002-5797-3722 AD - Gynecology and Obstetrics 1U, Physiopathology of Reproduction and IVF Unit, Department of Surgical Sciences, S. Anna Hospital, University of Turin, Turin, Italy. AD - Gynecology and Obstetrics 2U, Department of Surgical Sciences, S. Anna Hospital, University of Turin, Turin, Italy. FAU - Bergallo, Massimiliano AU - Bergallo M AUID- ORCID: 0000-0002-9291-1332 AD - Department of Public Health and Pediatric Sciences, University of Turin, Turin, Italy. AD - Pediatric Laboratory, Department of Children's Pathology and Care, Regina Margherita Children's Hospital, Turin, Italy. LA - eng GR - BERM_RILO_22_01/Universita degli Studi di Torino/ PT - Journal Article PL - Denmark TA - APMIS JT - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica JID - 8803400 RN - 0 (syncytin) RN - 0 (Gene Products, env) RN - 0 (Pregnancy Proteins) SB - IM MH - Humans MH - Female MH - *Endogenous Retroviruses/genetics MH - *Granulosa Cells/virology/metabolism MH - *Oocytes/virology/growth & development MH - *Epigenesis, Genetic MH - Gene Products, env/genetics MH - Pregnancy Proteins/genetics MH - Adult OTO - NOTNLM OT - assisted reproduction OT - epigenetic regulation OT - granulosa cells OT - human endogenous retroviruses OT - oocyte maturation EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 22:33 PHST- 2026/08/21 00:00 [revised] PHST- 2026/05/13 00:00 [received] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 22:33 [entrez] AID - e70276 [pii] AID - 10.1111/apm.70276 [doi] PST - ppublish SO - APMIS. 2026 Nov;134(11):e70276. doi: 10.1111/apm.70276. PMID- 42946535 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1745-1701 (Electronic) IS - 0586-7614 (Print) IS - 0586-7614 (Linking) VI - 52 IP - 6 DP - 2026 Oct 27 TI - Longitudinal Clinical and Functional Outcomes in Adult-Onset Delusional Disorder and Schizophrenia: A 10-Year Prospective Comparison Study. LID - 10.1093/schbul/sbag145 [doi] LID - sbag145 AB - BACKGROUND AND HYPOTHESIS: The distinction between delusional disorder (DD) and schizophrenia (SZ) remains debated, and onset age is an often-overlooked prognostic factor. We hypothesized that SZ would show greater diagnostic stability than DD, while functional and cognitive outcomes would be broadly comparable, with clinical differences emerging over time. STUDY DESIGN: Diagnostic stability over a decade was examined in an age-matched first-episode DD and SZ cohort (n = 142). 10-year clinical, functional, and cognitive outcomes were then compared among participants who retained their original diagnosis throughout follow-up (n = 111). Missing data were addressed using multiple imputation, with sensitivity analyses conducted in complete cases (n = 82) and deceased-excluded cases (n = 100). STUDY RESULTS: Over 10 years, 41% (29/71) of participants initially diagnosed with DD transitioned to SZ, compared with 3% (2/71) of those initially diagnosed with SZ who experienced a diagnostic shift. Among participants who retained their original diagnosis throughout follow-up (n = 111), SZ was associated with more relapses (B = 1.27, P = .012), hospitalizations (B = 0.47, P = .050), hallucinations (B = 3.35, P = .012), and perseverative errors on the modified Wisconsin Card Sorting Test (B = 4.31, P = .040), whereas DD showed poorer insight (Scale to Assess Unawareness of Mental Disorder: B = -1.38, P = .002). Sensitivity analyses yielded consistent results. CONCLUSION: These findings support a psychosis-spectrum model in which stable DD and SZ show substantial longitudinal overlap in functioning and cognition, despite differences in diagnostic stability and selected clinical outcomes. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Maryland Psychiatric Research Center. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Hui, Christy Lai Ming AU - Hui CLM AUID- ORCID: 0000-0001-6912-208X AD - Department of Psychiatry, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 0000, China. FAU - Wong, Charlie Cheuk Lam AU - Wong CCL AD - Department of Psychiatry, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 0000, China. FAU - Yang, Nicole Cai Lin AU - Yang NCL AUID- ORCID: 0009-0007-2407-5479 AD - Department of Psychiatry, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 0000, China. FAU - Chan, Evie Wai Ting AU - Chan EWT AD - Department of Psychiatry, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 0000, China. FAU - Suen, Yi Nam AU - Suen YN AUID- ORCID: 0000-0003-2766-8476 AD - School of Nursing, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 0000, China. FAU - Lee, Edwin Ho Ming AU - Lee EHM AD - Department of Psychiatry, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 0000, China. FAU - Chan, Sherry Kit Wa AU - Chan SKW AUID- ORCID: 0000-0003-2712-5826 AD - Department of Psychiatry, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 0000, China. FAU - Chang, Wing Chung AU - Chang WC AUID- ORCID: 0000-0002-3581-8895 AD - Department of Psychiatry, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 0000, China. FAU - Chan, Charles Wai Hong AU - Chan CWH AD - Department of Psychiatry, Pamela Youde Nethersole Eastern Hospital, Hong Kong, 0000, China. FAU - Yeung, Wai Song AU - Yeung WS AD - Department of Psychiatry, Pamela Youde Nethersole Eastern Hospital, Hong Kong, 0000, China. FAU - Siu, Man Wah AU - Siu MW AD - Department of Psychiatry, Kowloon Hospital, Hong Kong, 0000, China. FAU - Yan, Eric Wai Ching AU - Yan EWC AD - Department of Psychiatry, Kowloon Hospital, Hong Kong, 0000, China. FAU - Poon, Lap Tak AU - Poon LT AD - Department of Psychiatry, United Christian Hospital, Hong Kong, 0000, China. FAU - Pang, Pui Fai AU - Pang PF AD - Department of Psychiatry, United Christian Hospital, Hong Kong, 0000, China. FAU - Lo, Alison Wai Fan AU - Lo AWF AD - Department of Psychiatry, Kwai Chung Hospital, Hong Kong, 0000, China. FAU - Lee, Wing King AU - Lee WK AD - Department of Psychiatry, Kwai Chung Hospital, Hong Kong, 0000, China. FAU - Chiu, Mindi Wing Yan AU - Chiu MWY AD - Department of Psychiatry, Castle Peak Hospital, Hong Kong, 0000, China. FAU - Lam, Jenny Pik Ho AU - Lam JPH AD - Department of Psychiatry, Castle Peak Hospital, Hong Kong, 0000, China. FAU - Lui, Simon Sai Yu AU - Lui SSY AUID- ORCID: 0000-0001-9360-6244 AD - Department of Psychiatry, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 0000, China. AD - Department of Psychiatry, Castle Peak Hospital, Hong Kong, 0000, China. FAU - Wong, Yip Chau AU - Wong YC AD - Department of Psychiatry, Tai Po Hospital, Hong Kong, 0000, China. FAU - Chen, Eric Yu Hai AU - Chen EYH AD - Department of Psychiatry, School of Clinical Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, 0000, China. AD - Orygen, The National Centre of Excellence in Youth Mental Health, 35 Poplar Road Parkville, Victoria 3052, Australia. AD - Centre for Youth Mental Health, The University of Melbourne, 35 Poplar Road, Parkville, Victoria 3052, Australia. LA - eng GR - Health and Medical Research Fund/ GR - 17 181 581/Hong Kong Special Administrative Region/ PT - Comparative Study PT - Journal Article PL - United States TA - Schizophr Bull JT - Schizophrenia bulletin JID - 0236760 SB - IM MH - Humans MH - *Schizophrenia/physiopathology/complications/diagnosis MH - Female MH - Male MH - *Schizophrenia, Paranoid/physiopathology/diagnosis/complications MH - Adult MH - Longitudinal Studies MH - Middle Aged MH - Prospective Studies MH - *Disease Progression MH - Age of Onset MH - *Cognitive Dysfunction/etiology/physiopathology MH - Follow-Up Studies PMC - PMC13713149 OTO - NOTNLM OT - age of onset OT - diagnostic stability OT - first-episode psychosis OT - long-term outcomes OT - longitudinal follow-up studies OT - psychotic disorders COIS- E.Y.H.C. has participated in the paid advisory board for Otsuka and received educational grant support from Janssen-Cilag. The remaining authors declare no competing interests. EDAT- 2026/10/27 23:28 MHDA- 2026/10/28 05:28 PMCR- 2027/10/27 CRDT- 2026/10/27 22:03 PHST- 2026/03/25 00:00 [received] PHST- 2026/06/11 00:00 [revised] PHST- 2026/07/06 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 22:03 [entrez] PHST- 2027/10/27 00:00 [pmc-release] AID - 9003274 [pii] AID - sbag145 [pii] AID - 10.1093/schbul/sbag145 [doi] PST - ppublish SO - Schizophr Bull. 2026 Oct 27;52(6):sbag145. doi: 10.1093/schbul/sbag145. PMID- 42946534 OWN - NLM STAT- Publisher LR - 20261027 IS - 1522-9645 (Electronic) IS - 0195-668X (Linking) DP - 2026 Oct 28 TI - Cortisol in cardio-kidney-metabolic disease. LID - ehag827 [pii] LID - 10.1093/eurheartj/ehag827 [doi] AB - Despite guideline-directed therapy, many patients with cardio-kidney-metabolic (CKM) disease remain uncontrolled due to therapeutic inertia, adherence barriers, and underrecognized secondary causes. Hypercortisolism, ranging from mild autonomous cortisol secretion (MACS) to overt Cushing syndrome, is an increasingly recognized contributor. MACS is associated with a 2.5-fold higher risk of cardiovascular death and increased prevalence of hypertension, type 2 diabetes mellitus (T2DM), and kidney impairment. Cortisol excess promotes muscle insulin resistance, hepatic gluconeogenesis, beta-cell dysfunction, sodium retention, and vascular remodelling, worsening glycaemia and blood pressure, leading to poor CKM outcomes. Recent prospective studies suggest that hypercortisolism is more prevalent than previously suspected in selected CKM phenotypes, including 24% in patients with difficult-to-control T2DM in CATALYST and 27% in patients with resistant hypertension in MOMENTUM. The overnight 1 mg dexamethasone suppression test remains the diagnostic test of choice. Therapeutic strategies include adrenal steroidogenesis inhibitors, glucocorticoid receptor antagonists, and pituitary-directed therapies in selected cases, while surgery remains the first-line treatment for surgically remediable disease. This review summarizes the epidemiology and mechanisms linking cortisol excess to CKM dysfunction, and also highlights the need for clinical trials, standardized diagnostic thresholds, and assays to enable integration of cortisol-targeted therapies into management pathways to mitigate residual CKM risk. This review will serve as a practical guide for cardiologists to manage a potentially reversible contributor to poor CKM control. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the European Society of Cardiology. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Khan, Muhammad Shahzeb AU - Khan MS AUID- ORCID: 0000-0003-1250-6351 AD - Baylor Scott & White Research Institute, 3434 Oak Street St 501, Dallas, TX 75204, USA. AD - Division of Cardiology, Baylor Scott & White The Heart Hospital Plano, 1100 Allied Dr, Plano, TX 75093, USA. AD - Department of Medicine, Baylor College of Medicine, 702 SW H K Dodgen Loop, Temple, TX 76504, USA. FAU - Arshad, Muhammad Sameer AU - Arshad MS AD - Baylor Scott & White Research Institute, 3434 Oak Street St 501, Dallas, TX 75204, USA. FAU - Bhatt, Deepak L AU - Bhatt DL AUID- ORCID: 0000-0002-1278-6245 AD - Mount Sinai Fuster Heart Hospital, Icahn School of Medicine at Mount Sinai, New York, NY, USA. FAU - Cherney, David Z I AU - Cherney DZI AD - Division of Nephrology, Department of Medicine, Toronto General Hospital, Toronto, Ontario, Canada. FAU - DeFronzo, Ralph A AU - DeFronzo RA AUID- ORCID: 0000-0003-3839-1724 AD - Division of Diabetes, Department of Medicine, University of Texas Health Science Center, South Texas Veterans Health Care System and Texas Diabetes Institute, San Antonio, TX, USA. FAU - Green, Jennifer B AU - Green JB AD - Division of Endocrinology, Duke University School of Medicine, Duke Clinical Research Institute, Durham, NC, USA. FAU - Handelsman, Yehuda AU - Handelsman Y AD - Metabolic Institute of America, Tarzana, CA, USA. FAU - Shafi, Tariq AU - Shafi T AD - Division of Nephrology, Department of Medicine, Baylor Scott and White Health, Temple, TX, USA. FAU - Testani, Jeffrey AU - Testani J AD - Department of Internal Medicine, Yale University School of Medicine, New Haven, CT, USA. FAU - Butler, Javed AU - Butler J AUID- ORCID: 0000-0001-7683-4720 AD - Baylor Scott & White Research Institute, 3434 Oak Street St 501, Dallas, TX 75204, USA. AD - Department of Medicine, University of Mississippi Medical Center, Jackson, MS, USA. LA - eng PT - Journal Article DEP - 20261028 PL - England TA - Eur Heart J JT - European heart journal JID - 8006263 SB - IM OTO - NOTNLM OT - Cardio-kidney-metabolic disease OT - Cortisol OT - Cushing syndrome OT - Mild autonomous cortisol secretion EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 22:03 PHST- 2026/04/27 00:00 [received] PHST- 2026/08/10 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 22:03 [entrez] AID - 9003283 [pii] AID - 10.1093/eurheartj/ehag827 [doi] PST - aheadofprint SO - Eur Heart J. 2026 Oct 28:ehag827. doi: 10.1093/eurheartj/ehag827. PMID- 42946511 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1532-6497 (Electronic) IS - 1067-991X (Linking) VI - 45 IP - 6 DP - 2026 Nov-Dec TI - Peer Support for a Specialty Critical Care Transport Team. PG - 559-564 LID - S1067-991X(26)00228-2 [pii] LID - 10.1016/j.amj.2026.07.002 [doi] AB - OBJECTIVE: First responders experience higher rates of psychological distress than the general population, driven by repeated exposure to traumatic events and limited recovery time. Members of neonatal and pediatric critical care transport teams face additional cumulative stressors related to high-acuity patients and emotionally challenging transports. This study describes the development, implementation, and utilization of a peer-led support program within a hospital-based neonatal and pediatric critical care transport service. METHODS: Following a 2019 internal survey that identified a preference for peer-based emotional support, the Children's Mercy Critical Care Transport Team implemented a structured peer support program. Peer Support Persons (PSPs) were selected from a multidisciplinary transport team and completed standardized initial and ongoing training. Peer support was accessed through self-referral, leadership referral, and a confidential QR code (Quick Response Code) request system. Minimal, non-identifiable encounter data were tracked in a secure database. Descriptive analysis was used to evaluate program utilization from March 2023 to August 2025. RESULTS: During the study period, 209 peer support encounters were documented, including 11 formal debriefings and 199 informal interactions. Encounters involved registered nurses, respiratory therapists, emergency medical technicians, communication specialists, and other team members, most of which were related to difficult transports, patient deaths, and emotional distress. Program utilization increased over time, demonstrating the integration of peer support into routine team culture. Staff feedback was consistently positive, highlighting the value of timely, confidential, peer-led support. CONCLUSION: A structured, confidential, peer-led support program is feasible and sustainable in a neonatal and pediatric critical care transport setting. This model promotes resilience, reduces stigma, and may serve as a framework for similar first-responder programs. CI - Copyright (c) 2026 Air Medical Journal Associates. Published by Elsevier Inc. All rights reserved. FAU - Smith, Brenda S AU - Smith BS AD - Peer Support Co-Leader, Children's Mercy Critical Care Transport, Children's Mercy Hospital, Kansas City, MO. Electronic address: bssmith@cmh.edu. FAU - Pruitt, Lisa M AU - Pruitt LM AD - Quality Improvement Coordinator, Children's Mercy Critical Care Transport, Children's Mercy Hospital, Kansas City, MO. FAU - Flint, Jennifer L AU - Flint JL AD - Division of Critical Care, Department of Pediatrics, Children's Mercy Hospital, Kansas City, MO; Professor, University of Missouri-Kansas City School of Medicine, Kansas City, MO. LA - eng PT - Journal Article DEP - 20260826 PL - United States TA - Air Med J JT - Air medical journal JID - 9312325 SB - IM MH - Humans MH - *Peer Group MH - *Critical Care MH - *Patient Care Team MH - *Transportation of Patients MH - *Social Support MH - Stress, Psychological/prevention & control MH - *Air Ambulances MH - Emergency Medical Technicians COIS- Declaration of competing interest The authors have no relevant disclosures. There was no grant funding or financial support for this manuscript. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:53 PHST- 2026/04/13 00:00 [received] PHST- 2026/06/09 00:00 [revised] PHST- 2026/07/06 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:53 [entrez] AID - S1067-991X(26)00228-2 [pii] AID - 10.1016/j.amj.2026.07.002 [doi] PST - ppublish SO - Air Med J. 2026 Nov-Dec;45(6):559-564. doi: 10.1016/j.amj.2026.07.002. Epub 2026 Aug 26. PMID- 42946509 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1532-6497 (Electronic) IS - 1067-991X (Linking) VI - 45 IP - 6 DP - 2026 Nov-Dec TI - International Fixed-Wing Air Ambulance Repatriation: A Retrospective Observational Cohort Analysis With Regression Modeling of Fox Flight Air Ambulance Inc. Patient Repatriation Characteristics 2009 to 2024. PG - 543-549 LID - S1067-991X(26)00227-0 [pii] LID - 10.1016/j.amj.2026.07.001 [doi] AB - OBJECTIVE: As international travel rises globally, particularly among older adults with chronic conditions, illness or injury abroad may require air medical repatriation. Contemporary data describing patient characteristics, transport practices, and in-flight events are limited. The objective of this study is to describe the patient demographics, clinical indications, transport characteristics, and in-flight events associated with international fixed-wing air medical repatriation by a single Canadian provider. METHODS: We conducted a retrospective observational cohort study of all international repatriations completed by Fox Flight Air Ambulance Inc. between 2009 and 2024. Extracted chart data included demographics, diagnoses, transport modality, team composition, interventions, and in-flight events. Outcomes included repatriation completion, mortality, and in-flight events that required intervention. Multivariable logistic regression identified factors associated with in-flight events. RESULTS: A total of 3,905 transports were included. Patients were predominantly older adults (61.5 +/- 21.5 years), with orthopedic/trauma, cardiovascular, neurovascular, and infectious disease diagnoses comprising most repatriations. In-flight events occurred in 26.7% of transports; major events represented 15.0% of all events (3.9% of transports). Subgroup analyses demonstrated significant associations (P < .001) between in-flight interventions and mechanical ventilation, team composition, age group, and transport era. Over time, patients required stretchers and oxygen therapy more frequently, consistent with increasing acuity. In multivariable analysis, independent predictors of in-flight events included age, orthopedic/trauma and neoplasm-related diagnoses, oxygen therapy, invasive mechanical ventilation, and year of transport. CONCLUSION: Serious in-flight events were infrequent and occurred within transports staffed by experienced teams, although causality cannot be inferred. The findings support risk-informed planning, team composition decisions, and operational strategies to optimize safety and resource utilization. CI - Copyright (c) 2026 Air Medical Journal Associates. Published by Elsevier Inc. All rights reserved. FAU - Kao, Victoria AU - Kao V AD - Western University, Schulich School of Medicine and Dentistry, London, Ontario, Canada. FAU - Yeo, In Bum AU - Yeo IB AD - Western University, Schulich School of Medicine and Dentistry, London, Ontario, Canada. FAU - Priestap, Fran AU - Priestap F AD - London Health Sciences Centre, Divisions of Critical Care and Lawson Health Research Institute, London, Ontario, Canada. FAU - Fox, David AU - Fox D AD - Fox Flight Air Ambulance Inc., Toronto, Ontario, Canada. FAU - Ball, Ian AU - Ball I AD - Western University, Schulich School of Medicine and Dentistry, London, Ontario, Canada; London Health Sciences Centre, Divisions of Critical Care and Lawson Health Research Institute, London, Ontario, Canada; Department of Medicine, Western University, London, Ontario, Canada; Department of Epidemiology and Biostatistics, Western University, London, Ontario, Canada. FAU - Kao, Raymond AU - Kao R AD - Western University, Schulich School of Medicine and Dentistry, London, Ontario, Canada; London Health Sciences Centre, Divisions of Critical Care and Lawson Health Research Institute, London, Ontario, Canada; Department of Medicine, Western University, London, Ontario, Canada. Electronic address: Raymond.Kao@lhsc.on.ca. LA - eng PT - Journal Article PT - Observational Study DEP - 20260812 PL - United States TA - Air Med J JT - Air medical journal JID - 9312325 SB - IM MH - *Air Ambulances/statistics & numerical data MH - Retrospective Studies MH - Humans MH - Female MH - Aged MH - Male MH - Middle Aged MH - Canada MH - *Transportation of Patients/statistics & numerical data MH - Adult MH - Aged, 80 and over COIS- Declaration of competing interest The authors have no relevant disclosures. There was no grant funding or financial support for this manuscript. EDAT- 2026/10/27 23:28 MHDA- 2026/10/28 05:28 CRDT- 2026/10/27 21:53 PHST- 2026/01/27 00:00 [received] PHST- 2026/07/01 00:00 [revised] PHST- 2026/07/02 00:00 [accepted] PHST- 2026/10/28 05:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:53 [entrez] AID - S1067-991X(26)00227-0 [pii] AID - 10.1016/j.amj.2026.07.001 [doi] PST - ppublish SO - Air Med J. 2026 Nov-Dec;45(6):543-549. doi: 10.1016/j.amj.2026.07.001. Epub 2026 Aug 12. PMID- 42946471 OWN - NLM STAT- Publisher LR - 20261027 IS - 1535-5675 (Electronic) IS - 0091-4916 (Linking) DP - 2026 Oct 27 TI - From beta-Blasts to alpha-Bombs: Bone-Targeting Radiopharmaceutical Therapies. LID - jnmt.126.273620 [pii] LID - 10.2967/jnmt.126.273620 [doi] AB - Bone metastases are a significant source of morbidity in advanced malignancy, particularly in prostate cancer, because osteoblastic metastases can cause pain, skeletal-related events, and quality-of-life impairment. Bone-targeting radionuclide drugs are also effective in the treatment of increased bone turnover and were first developed as beta-emitters for palliation but have been developed in recent years as alpha-emitters with proven survival benefit. This review covers the biology, history, and mechanism of action of the approved bone-targeting radiopharmaceuticals ((89)Sr-chloride, (153)Sm-lexidronam, (223)Ra-dichloride). Special focus is placed on the only bone-targeting radiopharmaceutical currently approved in the United States-(223)Ra-dichloride-including patient selection, administration, radiation safety, and follow-up. (223)Ra, a calcium analog with a short alpha-particle path length, high linear energy transfer, and marrow-sparing properties, is valuable in the treatment of metastatic androgen pathway modulator-resistant prostate cancer (formerly metastatic castration-resistant prostate cancer) with bone-predominant disease and symptoms. The practical aspects of nuclear medicine are emphasized, such as imaging confirmation, laboratory value thresholds, written instructions, room preparation, contamination control, discharge instructions, and posttreatment monitoring. Bone-targeting radiopharmaceutical therapy has increasingly attractive options beyond palliation with new combination and sequencing treatment options involving prostate-specific membrane antigen-targeted therapy. The lessons learned from the shift from "beta-blasts" to "alpha-bombs" provide a potential pathway to more effective current treatment and a new generation of targeted radiopharmaceutical therapy. CI - (c) 2026 by the Society of Nuclear Medicine and Molecular Imaging. FAU - Chong, Nicole E AU - Chong NE AD - City of Hope National Medical Center, Duarte, California; nichong@coh.org. FAU - Wienhoff, Brieanne AU - Wienhoff B AD - Loma Linda University Medical Center, Loma Linda, California; and. FAU - Kuo, Nathan P AU - Kuo NP AD - Mayo Clinic Alix School of Medicine, Phoenix,Arizona. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Nucl Med Technol JT - Journal of nuclear medicine technology JID - 0430303 SB - IM OTO - NOTNLM OT - 223Ra-dichloride OT - bone-targeting radiopharmaceutical therapy OT - prostate cancer bone metastases EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 21:22 PHST- 2026/08/02 00:00 [received] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:22 [entrez] AID - jnmt.126.273620 [pii] AID - 10.2967/jnmt.126.273620 [doi] PST - aheadofprint SO - J Nucl Med Technol. 2026 Oct 27:jnmt.126.273620. doi: 10.2967/jnmt.126.273620. PMID- 42946468 OWN - NLM STAT- Publisher LR - 20261027 IS - 1535-5675 (Electronic) IS - 0091-4916 (Linking) DP - 2026 Oct 27 TI - Patient-Specific Dosimetry for [(131)I]NaI Therapy in End-Stage Renal Disease: Segmented Time-Activity Analysis, Hemodialysis Clearance, and Radiation Safety. LID - jnmt.126.272932 [pii] LID - 10.2967/jnmt.126.272932 [doi] AB - [(131)I]NaI therapy in patients with end-stage renal disease undergoing hemodialysis is challenging because renal clearance is markedly reduced, systemic retention is prolonged, and hemodialysis produces abrupt changes in activity. Patient-specific dosimetry may support individualized treatment planning and radiation-safety management. Methods: This prospective case series included 5 hemodialysis-dependent patients with end-stage renal disease and differentiated thyroid carcinoma treated with [(131)I]NaI under a standardized protocol combining scheduled hemodialysis with serial whole-body and blood measurements with family-dose and occupational radiation assessments. Whole-body activity was analyzed using a measurement-constrained segmented time-activity representation that explicitly accounted for dialysis-associated activity reductions. Blood activity concentration was integrated using trapezoidal integration with terminal monoexponential extrapolation. Whole-body and blood time-integrated activities, residence times, blood absorbed dose, red marrow absorbed dose, the modeled activity corresponding to a 2-Gy blood absorbed dose, postdischarge total effective dose equivalent, and occupational exposure were assessed. Results: Whole-body residence time ranged from 16.45 to 37.85 h, demonstrating substantial interpatient variability. Blood absorbed dose ranged from 0.054 to 0.452 Gy, and modeled red marrow absorbed dose ranged from 0.056 to 0.363 Gy. The modeled activities corresponding to a 2-Gy blood absorbed dose exceeded the administered activities but were not interpreted as evidence of activity escalation. Central postdischarge total effective dose equivalent estimates ranged from 0.13 to 2.47 mSv. All were below the 5-mSv constraint for comforters and caregivers, whereas cases 2 and 3 exceeded the 1-mSv constraint for other family members and had broad propagated uncertainties. Occupational exposure ranged from 5 to 158 muSv per hemodialysis session. Conclusion: In this 5-patient series, hemodialysis-adapted patient-specific dosimetry was feasible, and modeled blood and red marrow doses remained below the selected dosimetric constraint under the administered activities. The dosimetric workflow provides a practical framework for coordinating dosimetry, hemodialysis, and radiation safety but requires validation in larger multicenter prospective studies. CI - (c) 2026 by the Society of Nuclear Medicine and Molecular Imaging. FAU - Al Saidi, Huda AU - Al Saidi H AD - Department of Radiology and Nuclear Medicine, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman; and Sultan Qaboos University, Muscat, Oman h.alsaidi-1@cccrc.gov.om. FAU - Al Makhmari, Noura AU - Al Makhmari N AD - Department of Radiology and Nuclear Medicine, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman; and Sultan Qaboos University, Muscat, Oman. FAU - Al Maymani, Naema AU - Al Maymani N AD - Department of Radiology and Nuclear Medicine, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman; and Sultan Qaboos University, Muscat, Oman. FAU - Kheruka, Subhash Chand AU - Kheruka SC AD - Department of Radiology and Nuclear Medicine, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman; and Sultan Qaboos University, Muscat, Oman. FAU - Al Balushi, Anas AU - Al Balushi A AD - Department of Radiology and Nuclear Medicine, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman; and Sultan Qaboos University, Muscat, Oman. FAU - Al Rashdi, Sana AU - Al Rashdi S AD - Department of Radiology and Nuclear Medicine, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman; and Sultan Qaboos University, Muscat, Oman. FAU - Al Raii, Tasnim AU - Al Raii T AD - Department of Radiology and Nuclear Medicine, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman; and Sultan Qaboos University, Muscat, Oman. FAU - Al Riyami, Khulood AU - Al Riyami K AD - Department of Radiology and Nuclear Medicine, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman; and Sultan Qaboos University, Muscat, Oman. FAU - Al Sukaiti, Rashid AU - Al Sukaiti R AD - Department of Radiology and Nuclear Medicine, Sultan Qaboos Comprehensive Cancer Care and Research Center, University Medical City, Muscat, Oman; and Sultan Qaboos University, Muscat, Oman. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Nucl Med Technol JT - Journal of nuclear medicine technology JID - 0430303 SB - IM OTO - NOTNLM OT - [131I]NaI OT - dosimetry OT - hemodialysis OT - radiation safety OT - thyroid carcinoma EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 21:22 PHST- 2026/05/06 00:00 [received] PHST- 2026/08/17 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:22 [entrez] AID - jnmt.126.272932 [pii] AID - 10.2967/jnmt.126.272932 [doi] PST - aheadofprint SO - J Nucl Med Technol. 2026 Oct 27:jnmt.126.272932. doi: 10.2967/jnmt.126.272932. PMID- 42946457 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1743-498X (Electronic) IS - 1743-4971 (Print) IS - 1743-4971 (Linking) VI - 23 IP - 6 DP - 2026 Dec TI - Applying Trauma-Informed Principles to Support Learner Engagement and Wellbeing in UK Undergraduate Medical Education: A Qualitative Study. LID - e70540 [pii] LID - 10.1111/tct.70540 [doi] AB - INTRODUCTION: Trauma-informed education recognises the impact of psychological trauma and seeks to create environments that promote wellbeing and minimise further harm. In medical education, this approach has the potential to enhance student engagement, learning, professional development and overall health. This study explored how trauma-informed principles (safety, trust, support, collaboration, empowerment and social justice) are currently applied in undergraduate medical education and identified opportunities for broader implementation. METHODS: Thirty-eight medical educators from six UK medical schools participated in interactive workshops. Participants selected trauma-informed principles to discuss in small groups, guided by the prompts 'What are we already doing?' and 'What else could we do?' Data were analysed using template analysis. RESULTS: Six themes were identified that guide the application of a trauma-informed approach in undergraduate medical education: recognising student diversity and individuality; fostering connection and belonging; managing sensitive or challenging content; considering clinical experiences; involving students in decision-making; and the role of educators. DISCUSSION: Most participants were unfamiliar with trauma-informed approaches in medical education and valued the opportunity to reflect and share practices. Given that many medical students have experienced trauma or may do so during their training, integrating trauma-informed principles can help to mitigate its impact and support students. CONCLUSION: Trauma-informed principles promote equity, engagement and wellbeing, offering a valuable lens for improving medical education. CI - (c) 2026 The Author(s). The Clinical Teacher published by Association for the Study of Medical Education and John Wiley & Sons Ltd. FAU - West, Helen AU - West H AUID- ORCID: 0000-0001-8712-5890 AD - Department of Psychology, University of Liverpool, Liverpool, UK. FAU - Johnson, Dominic AU - Johnson D AUID- ORCID: 0009-0004-4032-2802 AD - UCL Medical School, Faculty of Medical Sciences, University College London, London, England. LA - eng PT - Journal Article PL - England TA - Clin Teach JT - The clinical teacher JID - 101227511 SB - IM MH - Humans MH - *Education, Medical, Undergraduate/organization & administration/methods MH - United Kingdom MH - Qualitative Research MH - *Students, Medical/psychology MH - *Psychological Trauma/psychology MH - Psychological Safety MH - Academia MH - Learning PMC - PMC13713221 OTO - NOTNLM OT - medical education OT - qualitative OT - trauma OT - trauma-informed OT - undergraduate COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 PMCR- 2026/10/28 CRDT- 2026/10/27 21:13 PHST- 2026/08/25 00:00 [revised] PHST- 2025/09/12 00:00 [received] PHST- 2026/09/11 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:13 [entrez] PHST- 2026/10/28 00:00 [pmc-release] AID - e70540 [pii] AID - TCT70540 [pii] AID - 10.1111/tct.70540 [doi] PST - ppublish SO - Clin Teach. 2026 Dec;23(6):e70540. doi: 10.1111/tct.70540. PMID- 42946456 OWN - NLM STAT- Publisher LR - 20261027 IS - 1749-4486 (Electronic) IS - 1749-4478 (Linking) DP - 2026 Oct 27 TI - Risk of Malignancy in Asymptomatic Unilateral Tonsillar Enlargement in Adults and Children: A Systematic Review and Meta-Analysis. LID - 10.1111/coa.70189 [doi] AB - BACKGROUND: Unilateral tonsillar enlargement (UTE) may raise concern for malignancy and frequently prompt diagnostic tonsillectomy. However, when present in the absence of additional suspicious features, the true risk of malignancy remains uncertain. This study aimed to evaluate the prevalence of malignant histology in patients undergoing tonsillectomy for isolated asymptomatic UTE. METHODS: A systematic review was conducted in accordance with PRISMA guidance. MEDLINE, EMBASE, EMCARE, PubMed and the Cochrane Library were searched from inception to 22 September 2025. Studies reporting histopathological outcomes in patients undergoing tonsillectomy for isolated asymptomatic UTE were included. A meta-analysis of proportions was performed for adult-only cohorts. RESULTS: Eighteen studies comprising 1582 tonsillectomy specimens met the inclusion criteria. No malignant cases were identified in paediatric patients (0/751; 95% CI 0.00%-0.49%). Among adult patients (n = 712), six malignant cases were identified, all of which were lymphoproliferative in origin. Meta-analysis of adult-only studies demonstrated a pooled prevalence of 2.04% (95% CI 0.98%-4.22%) with low heterogeneity (I(2) = 15.2%). The crude overall prevalence of malignancy across all cohorts was 0.38% (95% CI 0.14%-0.82%). CONCLUSION: Isolated asymptomatic UTE is associated with an extremely low risk of malignancy, particularly in paediatric patients. Routine diagnostic tonsillectomy is unlikely to be justified in children and should be selectively considered in adults based on clinical risk factors. Careful clinical assessment remains essential in guiding management. TRIAL REGISTRATION: PROSPERO: CRD420261423941. CI - (c) 2026 John Wiley & Sons Ltd. FAU - Khan, Hamad D AU - Khan HD AUID- ORCID: 0009-0000-7974-0244 AD - Department of Otorhinolaryngology and Head and Neck Surgery, Birmingham Women's and Children's NHS Foundation Trust, Birmingham, UK. FAU - Fahmy, Miriam AU - Fahmy M AUID- ORCID: 0000-0003-1882-6380 AD - Department of Otorhinolaryngology and Head and Neck Surgery, Dudley Group NHS Foundation Trust, Dudley, UK. FAU - Javed, Azfar AU - Javed A AUID- ORCID: 0000-0001-7522-5202 AD - Department of Otorhinolaryngology and Head and Neck Surgery, University Hospitals of North Midlands NHS Trust, Stoke-on-Trent, UK. FAU - Abou-Foul, Ahmad K AU - Abou-Foul AK AUID- ORCID: 0000-0002-5321-5465 AD - Institute of Head and Neck Studies and Education (InHANSE), Department of Cancer and Genomic Sciences, University of Birmingham, Birmingham, UK. FAU - McDermott, Ann-Louise AU - McDermott AL AUID- ORCID: 0000-0002-8903-9803 AD - Department of Otorhinolaryngology and Head and Neck Surgery, Birmingham Women's and Children's NHS Foundation Trust, Birmingham, UK. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - England TA - Clin Otolaryngol JT - Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery JID - 101247023 SB - IM OTO - NOTNLM OT - diagnostic tonsillectomy OT - head and neck cancer OT - lymphoma OT - occult malignancy OT - tonsillar asymmetry OT - tonsillectomy histology OT - unilateral tonsillar enlargement EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 21:13 PHST- 2026/06/24 00:00 [revised] PHST- 2026/06/24 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:13 [entrez] AID - 10.1111/coa.70189 [doi] PST - aheadofprint SO - Clin Otolaryngol. 2026 Oct 27. doi: 10.1111/coa.70189. PMID- 42946447 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - Breast Cancer Side Population Versus CD44(+)/CD24(-) Cells: Functional and Tumorigenic Differences. PG - 6041-6050 LID - 10.21873/anticanres.18436 [doi] AB - BACKGROUND/AIM: Cancer stem cells (CSCs) are heterogeneous and exhibit distinct phenotypic and functional characteristics. The side population (SP) and CD44(+)/CD24(-) phenotypes are widely used to enrich breast cancer cells with CSC-associated properties; however, whether these approaches identify functionally equivalent populations remains unclear. Building on our previous establishment of a DyeCycle Violet (DCV)-based method for isolating SP cells from MCF-7 breast cancer cells, this study compared the biological characteristics of DCV-defined SP cells and a CD44(+)/CD24(-)-enriched fraction. MATERIALS AND METHODS: SP cells were isolated from MCF-7 cells by fluorescence-activated cell sorting using DCV and fumitremorgin C. A CD44(+)/CD24(-)-enriched fraction was isolated by magnetic-activated cell sorting. Parental MCF-7 cells, SP cells, and the CD44(+)/CD24(-)-enriched fraction were compared for proliferation and invasion. Tumor-forming ability was evaluated in nude mice following subcutaneous inoculation of 100 or 500 cells. Xenograft tumors were examined histopathologically and immunohistochemically. RESULTS: No significant differences in proliferative activity were observed among parental MCF-7, SP, and the CD44(+)/CD24(-)-enriched fraction at either 48 or 72 h. Invasion was highest in the CD44(+)/CD24(-)-enriched fraction (87.63+/-20.29%), followed by SP cells (51.86+/-6.83%) and parental MCF-7 cells (32.52+/-9.95%; p=0.001). Tumors developed in all three mice receiving the CD44(+)/CD24(-)-enriched fraction at both cell doses, whereas tumor formation was less frequent in the other groups. Xenograft tumors showed morphological and immunohistochemical characteristics consistent with MCF-7 cells. CONCLUSION: DCV-defined SP cells and the CD44(+)/CD24(-)-enriched fraction exhibited distinct biological characteristics. The CD44(+)/CD24(-)-enriched fraction showed high invasive activity and frequent tumor formation, whereas SP cells showed relatively high proliferative activity and retained tumor-forming capacity under the experimental conditions examined. These findings suggest that SP and CD44(+)/CD24(-) enrichment methods select populations with different functional properties. CI - Copyright (c) 2026 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Osawa, Yukihiko AU - Osawa Y AD - Graduate School of Health Sciences, Department of Health Sciences, Kyoto Tachibana University, Kyoto, Japan; osawa-y@tachibana-u.ac.jp. AD - Department of Medical Technology and Sciences, Faculty of Health Sciences, Kyoto Tachibana University, Kyoto, Japan. AD - Research Center for Life and Health Sciences, Kyoto Tachibana University, Kyoto, Japan. FAU - Kumazaki, Aya AU - Kumazaki A AD - Department of Clinical Laboratory, TOYOTA Memorial Hospital, Aichi, Japan. FAU - Imanaka, Konatsu AU - Imanaka K AD - Graduate School of Health Sciences, Department of Health Sciences, Kyoto Tachibana University, Kyoto, Japan. FAU - Ohmoto, Yuki AU - Ohmoto Y AD - Graduate School of Health Sciences, Department of Health Sciences, Kyoto Tachibana University, Kyoto, Japan. FAU - Yokoe, Maika AU - Yokoe M AD - Graduate School of Health Sciences, Department of Health Sciences, Kyoto Tachibana University, Kyoto, Japan. FAU - Odashima, Hirokazu AU - Odashima H AD - Department of Medical Technology and Sciences, Faculty of Health Sciences, Kyoto Tachibana University, Kyoto, Japan. AD - Research Center for Life and Health Sciences, Kyoto Tachibana University, Kyoto, Japan. FAU - Onishi, Takafumi AU - Onishi T AD - Department of Medical Technology and Sciences, Faculty of Health Sciences, Kyoto Tachibana University, Kyoto, Japan. AD - Research Center for Life and Health Sciences, Kyoto Tachibana University, Kyoto, Japan. FAU - Hattori, Manabu AU - Hattori M AD - Graduate School of Health Sciences, Department of Health Sciences, Kyoto Tachibana University, Kyoto, Japan. AD - Department of Medical Technology and Sciences, Faculty of Health Sciences, Kyoto Tachibana University, Kyoto, Japan. AD - Research Center for Life and Health Sciences, Kyoto Tachibana University, Kyoto, Japan. LA - eng PT - Journal Article PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 RN - 0 (CD24 Antigen) RN - 0 (Hyaluronan Receptors) RN - 0 (CD24 protein, human) RN - 0 (CD44 protein, human) SB - IM MH - Humans MH - *CD24 Antigen/metabolism MH - *Hyaluronan Receptors/metabolism MH - Female MH - Animals MH - *Breast Neoplasms/pathology/metabolism MH - MCF-7 Cells MH - *Neoplastic Stem Cells/metabolism/pathology MH - Mice, Nude MH - Mice MH - *Side-Population Cells/pathology/metabolism MH - Cell Proliferation MH - Flow Cytometry OTO - NOTNLM OT - Breast cancer OT - CD24 OT - CD44 OT - MCF-7 OT - cancer stem cells OT - invasion OT - side population OT - tumorigenicity EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:04 PHST- 2026/08/27 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:04 [entrez] AID - 46/11/6041 [pii] AID - 10.21873/anticanres.18436 [doi] PST - ppublish SO - Anticancer Res. 2026 Nov;46(11):6041-6050. doi: 10.21873/anticanres.18436. PMID- 42946446 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - Clinical Outcomes of Postoperative Three-weekly Cisplatin for High-risk Head and Neck Squamous Cell Carcinoma. PG - 6287-6296 LID - 10.21873/anticanres.18457 [doi] AB - BACKGROUND/AIM: Microscopic positive margins and extranodal extension are established pathological risk factors for recurrence after curative surgery for head and neck squamous cell carcinoma. Postoperative radiotherapy with concurrent cisplatin is the standard treatment for such patients. We evaluated the outcomes of postoperative radiotherapy administered concurrently with three-weekly cisplatin and explored factors associated with disease-free survival. PATIENTS AND METHODS: We retrospectively analyzed patients with head and neck squamous cell carcinoma who started postoperative radiotherapy with concurrent cisplatin between April 2015 and December 2023. Eligible patients had microscopic positive margins or extranodal extension and received postoperative radiotherapy concurrently with three-weekly cisplatin. The primary endpoint was disease-free survival. Secondary endpoints included overall survival, recurrence patterns, and factors associated with disease-free survival. RESULTS: We included 83 patients (median age, 62 years; 68 men). The 5-year disease-free and overall survival rates were 55.5% and 67.9%, respectively, and neither median was reached. Distant metastasis developed in 23 patients (27.7%), with 1-year and 5-year cumulative rates of 21.3% and 28.8%, respectively. In univariate analysis, radiotherapy dose <60 Gy, perineural invasion, and venous invasion were associated with poorer disease-free survival. Disease-free survival did not differ according to cumulative cisplatin dose (>/=200 vs. <200 mg/m(2)). CONCLUSION: Postoperative radiotherapy administered concurrently with three-weekly cisplatin was associated with 5-year disease-free and overall survival rates of 55.5% and 67.9%, respectively, in routine clinical practice. Perineural and venous invasion may provide additional prognostic information beyond established high-risk pathological features. The frequency of distant metastasis suggests the need for strategies that improve both locoregional and systemic control. CI - Copyright (c) 2026 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Aihara, Yusuke AU - Aihara Y AD - Department of Otorhinolaryngology, Head and Neck Surgery, Tokyo Medical University, Tokyo, Japan; ahrysk@gmail.com. FAU - Tanaka, Hideki AU - Tanaka H AD - Department of Otorhinolaryngology, Head and Neck Surgery, Tokyo Medical University, Tokyo, Japan. FAU - Hihara, Kosuke AU - Hihara K AD - Department of Otorhinolaryngology, Head and Neck Surgery, Tokyo Medical University, Tokyo, Japan. FAU - Hanyu, Kenji AU - Hanyu K AD - Department of Otorhinolaryngology, Head and Neck Surgery, Tokyo Medical University, Tokyo, Japan. FAU - Ueda, Yuri AU - Ueda Y AD - Department of Otorhinolaryngology, Head and Neck Surgery, Tokyo Medical University, Tokyo, Japan. FAU - Okamoto, Isaku AU - Okamoto I AD - Department of Otorhinolaryngology, Head and Neck Surgery, Tokyo Medical University, Tokyo, Japan. FAU - Zama, Atsuhiko AU - Zama A AD - Department of Radiology, Tokyo Medical University, Tokyo, Japan. FAU - Itonaga, Tomohiro AU - Itonaga T AD - Department of Radiology, Tokyo Medical University, Tokyo, Japan. FAU - Tsukahara, Kiyoaki AU - Tsukahara K AD - Department of Otorhinolaryngology, Head and Neck Surgery, Tokyo Medical University, Tokyo, Japan. LA - eng PT - Journal Article PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 RN - Q20Q21Q62J (Cisplatin) RN - 0 (Antineoplastic Agents) SB - IM MH - Humans MH - *Cisplatin/administration & dosage/therapeutic use MH - Middle Aged MH - Male MH - *Squamous Cell Carcinoma of Head and Neck/therapy/mortality/pathology/drug therapy MH - Female MH - Retrospective Studies MH - *Head and Neck Neoplasms/therapy/pathology/mortality/drug therapy MH - Disease-Free Survival MH - Aged MH - Neoplasm Recurrence, Local MH - *Antineoplastic Agents/administration & dosage/therapeutic use MH - Treatment Outcome MH - Adult OTO - NOTNLM OT - Head and neck squamous cell carcinoma OT - cisplatin OT - extranodal extension OT - perineural invasion OT - positive margin OT - postoperative chemoradiotherapy OT - venous invasion EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:04 PHST- 2026/08/06 00:00 [received] PHST- 2026/08/31 00:00 [revised] PHST- 2026/09/02 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:04 [entrez] AID - 46/11/6287 [pii] AID - 10.21873/anticanres.18457 [doi] PST - ppublish SO - Anticancer Res. 2026 Nov;46(11):6287-6296. doi: 10.21873/anticanres.18457. PMID- 42946445 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - High Ubiquitin-like Protein 3 Expression Is Associated With Prolonged Recurrence-free Survival in Non-muscle-invasive Bladder Cancer. PG - 6249-6263 LID - 10.21873/anticanres.18454 [doi] AB - BACKGROUND/AIM: Current clinicopathological factors inadequately capture the biological heterogeneity of non-muscle-invasive bladder cancer (NMIBC). This study investigated the prognostic significance of ubiquitin-like protein 3 (UBL3), a molecule involved in extracellular vesicle sorting and immune regulation, in NMIBC. MATERIALS AND METHODS: Following an exploratory analysis of public RNA-sequencing databases, we evaluated tissue samples from patients who underwent transurethral resection for primary NMIBC. A discovery cohort (n=37) was assessed for UBL3 mRNA via qRT-PCR and protein expression via immunohistochemistry (IHC) quantified using digital H-scores (QuPath). A separate internal validation cohort (n=192) was subsequently evaluated by IHC. The primary endpoint was recurrence-free survival (RFS). RESULTS: The database analysis indicated that high UBL3 expression was associated with longer RFS in early-stage bladder cancer. In the discovery cohort, higher UBL3 mRNA levels were associated with prolonged RFS [hazard ratio (HR)=0.33, p=0.004]. mRNA levels also were associated with digital H-scores (rho=0.39, p=0.018). In the separate internal validation cohort, elevated UBL3 protein expression was associated with a reduced risk of recurrence. A multivariable Cox regression confirmed that a higher UBL3 H-score (HR=0.95 per 10-point increase, p=0.023), alongside a negative preoperative urine cytology and immediate intravesical chemotherapy, remained independently associated with RFS. CONCLUSION: Elevated UBL3 expression was associated with prolonged RFS in patients with NMIBC following transurethral resection of bladder tumor. While UBL3 has potential as a prognostic biomarker, establishing its definitive clinical utility will require mechanistic investigations and validation across independent, multicenter cohorts. CI - Copyright (c) 2026 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Sano, Asuka AU - Sano A AD - Department of Urology, Hamamatsu University School of Medicine, Hamamatsu, Japan; askxxx1130@gmail.com. FAU - Tamura, Keita AU - Tamura K AD - Department of Urology, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Sakai, Yasuhiro AU - Sakai Y AD - Department of Tumor Pathology, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Kahyo, Tomoaki AU - Kahyo T AD - Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Hirayama, Shoshiro AU - Hirayama S AD - Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Sekihara, Keigo AU - Sekihara K AD - First Department of Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Takanashi, Yusuke AU - Takanashi Y AD - First Department of Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Watanabe, Shunsuke AU - Watanabe S AD - Department of Urology, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Watanabe, Kyohei AU - Watanabe K AD - Department of Urology, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Matsushita, Yuto AU - Matsushita Y AD - Department of Urology, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Watanabe, Hiromitsu AU - Watanabe H AD - Department of Urology, Hamamatsu University School of Medicine, Hamamatsu, Japan. AD - Department of Developed Studies for Advanced Robotic Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Motoyama, Daisuke AU - Motoyama D AD - Department of Urology, Hamamatsu University School of Medicine, Hamamatsu, Japan. AD - Department of Developed Studies for Advanced Robotic Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Otsuka, Atsushi AU - Otsuka A AD - Department of Urology, Iwata City Hospital, Iwata, Japan. FAU - Inamoto, Teruo AU - Inamoto T AD - Department of Urology, Hamamatsu University School of Medicine, Hamamatsu, Japan. FAU - Setou, Mitsutoshi AU - Setou M AD - Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, Hamamatsu, Japan. LA - eng PT - Journal Article PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 RN - 0 (Biomarkers, Tumor) RN - 0 (Ubiquitins) SB - IM MH - Humans MH - *Non-Muscle Invasive Bladder Neoplasms/genetics/pathology/mortality/metabolism/surgery MH - Female MH - Male MH - Prognosis MH - Aged MH - *Biomarkers, Tumor/genetics/metabolism MH - Transurethral Resection of Bladder MH - *Neoplasm Recurrence, Local/genetics/pathology MH - Middle Aged MH - Disease-Free Survival MH - *Ubiquitins/genetics/metabolism MH - *Urinary Bladder Neoplasms/genetics/pathology MH - Gene Expression Regulation, Neoplastic OTO - NOTNLM OT - Non-muscle-invasive bladder cancer OT - UBL3 OT - immunohistochemistry OT - prognostic biomarker OT - recurrence-free survival EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:04 PHST- 2026/08/13 00:00 [received] PHST- 2026/09/08 00:00 [revised] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:04 [entrez] AID - 46/11/6249 [pii] AID - 10.21873/anticanres.18454 [doi] PST - ppublish SO - Anticancer Res. 2026 Nov;46(11):6249-6263. doi: 10.21873/anticanres.18454. PMID- 42946443 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - Feasibility and Short-term Outcomes of Robotic Surgery for Transverse Colon Cancer: A Multicenter Retrospective Study. PG - 6427-6435 LID - 10.21873/anticanres.18469 [doi] AB - BACKGROUND/AIM: Patients with transverse colon cancer have been excluded from randomized controlled trials due to its anatomical complexity and high technical difficulty. Few studies have compared robotic surgery with laparoscopic or open surgery for transverse colon cancer. We conducted a comparative analysis of the safety and efficacy of robotic surgery for transverse colon cancer. PATIENTS AND METHODS: We retrospectively reviewed 635 patients treated for transverse colon cancer between 2016 and 2025. Patients were categorized according to the surgical approach: robotic surgery (Robot group, n=38), laparoscopic surgery (Lap group, n=530), and open surgery (Open group, n=67). Clinicopathological and surgical features were compared between groups. RESULTS: Operative time was longer (Robot vs. Lap vs. Open; 233 vs. 226 vs. 181 min, p<0.001), blood loss was lower (15 vs. 20 vs. 120 ml, p<0.001), more lymph nodes were retrieved (19 vs. 16 vs. 10, p=0.002), and hospital stay was shorter (9 vs. 12 vs. 22 days, p<0.001) in the Robot group. The postoperative complication rate tended to be lower in the Robot group (7.9% vs. 23.2% vs. 25.4%, p=0.077). In multivariate analysis, male sex (OR=1.559, 95% CI=1.056-2.301, p=0.025) and ASA-PS 3 (OR=2.486, 95% CI=1.460-4.232, p<0.001) were identified as independent risk factors for postoperative complications. CONCLUSION: Although favorable short-term outcomes were observed in this small, highly selected robotic cohort, these descriptive findings do not establish the clinical superiority or equivalence of robotic surgery over laparoscopic or open surgery. Larger prospective studies are needed to define its oncological and surgical value. CI - Copyright (c) 2026 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Shiraishi, Toshio AU - Shiraishi T AD - Department of Surgery, Isahaya General Hospital, Nagasaki, Japan. FAU - Tominaga, Tetsuro AU - Tominaga T AD - Department of Surgery, Isahaya General Hospital, Nagasaki, Japan. AD - Division of Surgical Oncology, Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. AD - Colorectal Surgery, Department of Surgery, Nagasaki University Hospital, Nagasaki, Japan. FAU - Wakata, Koki AU - Wakata K AD - Department of Surgery, Isahaya General Hospital, Nagasaki, Japan. FAU - Nishimuta, Masato AU - Nishimuta M AD - Department of Surgery, Isahaya General Hospital, Nagasaki, Japan. FAU - Utsugi, Masaki AU - Utsugi M AD - Department of Surgery, Isahaya General Hospital, Nagasaki, Japan. FAU - Fukuoka, Hidetoshi AU - Fukuoka H AD - Department of Surgery, Isahaya General Hospital, Nagasaki, Japan. FAU - Ishii, Mitsutoshi AU - Ishii M AD - Division of Surgical Oncology, Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. AD - Colorectal Surgery, Department of Surgery, Nagasaki University Hospital, Nagasaki, Japan. AD - Minimally Invasive Surgery Center, Nagasaki University Hospital, Nagasaki, Japan. FAU - Noda, Keisuke AU - Noda K AD - Division of Surgical Oncology, Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. AD - Colorectal Surgery, Department of Surgery, Nagasaki University Hospital, Nagasaki, Japan. AD - Minimally Invasive Surgery Center, Nagasaki University Hospital, Nagasaki, Japan. FAU - Hashimoto, Shintaro AU - Hashimoto S AD - Department of Surgery, Sasebo City General Hospital, Nagasaki, Japan. FAU - Oishi, Kaido AU - Oishi K AD - Department of Surgery, National Hospital Organization Nagasaki Medical Center, Nagasaki, Japan. FAU - Taguchi, Koya AU - Taguchi K AD - Department of Surgery, Ureshino Medical Center, Saga, Japan. FAU - Oyama, Shosaburo AU - Oyama S AD - Department of Surgery, Saiseikai Nagasaki Hospital, Nagasaki, Japan. FAU - Inao, Ayano AU - Inao A AD - Department of Surgery, Saseno Chuo Hospital, Sasebo, Japan. FAU - Nonaka, Takashi AU - Nonaka T AD - Division of Surgical Oncology, Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan; tnonaka@nagasaki-u.ac.jp. AD - Colorectal Surgery, Department of Surgery, Nagasaki University Hospital, Nagasaki, Japan. AD - Minimally Invasive Surgery Center, Nagasaki University Hospital, Nagasaki, Japan. FAU - Matsumoto, Keitaro AU - Matsumoto K AD - Division of Surgical Oncology, Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. LA - eng PT - Journal Article PT - Multicenter Study PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 SB - IM MH - Humans MH - *Robotic Surgical Procedures/methods/adverse effects MH - Male MH - *Colonic Neoplasms/surgery/pathology MH - Retrospective Studies MH - Female MH - Aged MH - Treatment Outcome MH - Laparoscopy/methods/adverse effects MH - Middle Aged MH - Postoperative Complications/etiology MH - Feasibility Studies MH - *Colon, Transverse/surgery/pathology MH - Operative Time MH - Length of Stay MH - Aged, 80 and over MH - Colorectal Surgical Procedures OTO - NOTNLM OT - Perioperative outcomes OT - multicenter study OT - postoperative complications OT - robotic surgery OT - transverse colon cancer EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:04 PHST- 2026/07/10 00:00 [received] PHST- 2026/08/21 00:00 [revised] PHST- 2026/09/03 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:04 [entrez] AID - 46/11/6427 [pii] AID - 10.21873/anticanres.18469 [doi] PST - ppublish SO - Anticancer Res. 2026 Nov;46(11):6427-6435. doi: 10.21873/anticanres.18469. PMID- 42946437 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - Familial Glioma in a Father and Son: Integrated Genomic and Multi-compartment Proteomic Profiling. PG - 5997-6015 LID - 10.21873/anticanres.18433 [doi] AB - BACKGROUND/AIM: Genetic predisposition to glioblastoma multiforme (GBM) is rare and is often associated with syndromes such as Li-Fraumeni syndrome and neurofibromatosis. However, a subset of cases involves familial aggregation of glioma, and its molecular pathogenesis remains poorly understood. This study aimed to elucidate the proteomic and genomic profiles of familial glioma cases. CASE REPORT: A 60-year-old male (father) and his 35-year-old son presented with gliomas. Clinical histories, imaging, surgical procedures, and histological analyses were documented. Proteomic analysis of tissue, plasma-derived extracellular vesicles (EVs), and urine was performed, and a systems biology approach was used to compare molecular profiles. Computational evaluation and pathway analysis were performed. Gliomas were diagnosed according to World Health Organization (WHO) guidelines. The father was diagnosed with glioblastoma, isocitrate dehydrogenase (IDH)-wildtype, WHO grade 4, with an unmethylated O6-methylguanine-DNA methyltransferase (MGMT) promoter, whereas the son presented with astrocytoma, IDH-mutant, WHO grade 3, with a methylated MGMT promoter. Proteomic analysis identified 1,268 proteins, with approximately 200 differentially abundant proteins (DAPs) in each biological matrix. Pathway analysis highlighted patient-specific differences, including inflammatory markers and GBM-associated proteins, with particularly notable variation in urine samples, including glycoproteins. CONCLUSION: This study provides insight into proteomic differences between familial gliomas, showing that they can develop through distinct molecular profiles and pathogenic pathways despite a shared genetic background. Proteomic analysis across tissue, plasma-derived EVs, and urine revealed tumor-associated proteins that could serve as non-invasive biomarkers, capturing patient-specific molecular features that were less evident in plasma. CI - Copyright (c) 2026 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Umana, Giuseppe E AU - Umana GE AD - Department of Neurosurgery, Trauma Center, Gamma Knife Center, Cannizzaro Hospital, Catania, Italy; giuseppe.umana@unikore.it. AD - Department of Medicine and Surgery, Kore University of Enna, Enna, Italy. FAU - Ciuffreda, Giusy AU - Ciuffreda G AD - Institute of Biomedical Technologies National Research Council (ITB-CNR), Segrate, Italy. AD - Universita degli Studi di Padua, Dipartimento di Scienze Biomediche Complesso Vallisneri, Padua, Italy. FAU - Cortese, Elena AU - Cortese E AD - Department of Neurosurgery, Trauma Center, Gamma Knife Center, Cannizzaro Hospital, Catania, Italy. FAU - Corso, Chiara AU - Corso C AD - Department of Neurosurgery, Trauma Center, Gamma Knife Center, Cannizzaro Hospital, Catania, Italy. FAU - Raffa, Giovanni AU - Raffa G AD - Neurosurgery Unit, Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, Messina, Italy. FAU - Vecchio, Claudia Rita AU - Vecchio CR AD - Department of Medicine and Surgery, Kore University of Enna, Enna, Italy. AD - MCAU, Ospedale Cannizzaro, Catania, Italy. FAU - Ranganathan, Sruthi AU - Ranganathan S AD - School of Medicine, Cambridge University, Cambridge, U.K. FAU - Pettinato, Angela AU - Pettinato A AD - Italy Pathology Unit - Cannizzaro Hospital, Catania, Italy. FAU - Curduman, Mara AU - Curduman M AD - Italy Pathology Unit - Cannizzaro Hospital, Catania, Italy. FAU - Spadola, Saveria AU - Spadola S AD - Department of Medicine and Surgery, Kore University of Enna, Enna, Italy. AD - Italy Pathology Unit - Cannizzaro Hospital, Catania, Italy. FAU - Ferini, Gianluca AU - Ferini G AD - Department of Neurosurgery, Trauma Center, Gamma Knife Center, Cannizzaro Hospital, Catania, Italy. AD - Department of Radiation Oncology, REM Radioterapia srl, Viagrande, Italy. FAU - Vigano, Raffaello AU - Vigano R AD - Institute of Biomedical Technologies National Research Council (ITB-CNR), Segrate, Italy. FAU - Brambilla, Francesca AU - Brambilla F AD - Institute of Biomedical Technologies National Research Council (ITB-CNR), Segrate, Italy. FAU - DI Silvestre, Dario AU - DI Silvestre D AD - Institute of Biomedical Technologies National Research Council (ITB-CNR), Segrate, Italy. FAU - Torrente, Yvan AU - Torrente Y AD - Neurology Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy. AD - Dino Ferrari Center, Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy. FAU - Mauri, Pierluigi AU - Mauri P AD - Institute of Biomedical Technologies National Research Council (ITB-CNR), Segrate, Italy. AD - Institute of Endotypes in Oncology Metabolism and Immunology "G. Salvatore" (IEOMI-CNR), Naples, Italy. LA - eng PT - Case Reports PT - Journal Article PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 RN - EC 6.5.1.- (DNA Repair Enzymes) RN - 0 (Tumor Suppressor Proteins) RN - 0 (Biomarkers, Tumor) RN - EC 2.1.1.- (DNA Modification Methylases) RN - EC 1.1.1.41 (Isocitrate Dehydrogenase) SB - IM MH - Humans MH - Male MH - Middle Aged MH - *Proteomics/methods MH - Adult MH - *Brain Neoplasms/genetics/metabolism/pathology MH - Genomics/methods MH - Genetic Predisposition to Disease MH - DNA Repair Enzymes/genetics MH - *Glioma/genetics/metabolism/pathology MH - Tumor Suppressor Proteins/genetics MH - Biomarkers, Tumor/genetics MH - *Glioblastoma/genetics MH - DNA Modification Methylases/genetics MH - Isocitrate Dehydrogenase/genetics MH - DNA Methylation OTO - NOTNLM OT - Glioma OT - familial aggregation OT - molecular pathogenesis OT - personalized treatment OT - proteomic analysis EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:04 PHST- 2026/07/16 00:00 [received] PHST- 2026/08/05 00:00 [revised] PHST- 2026/09/14 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:04 [entrez] AID - 46/11/5997 [pii] AID - 10.21873/anticanres.18433 [doi] PST - ppublish SO - Anticancer Res. 2026 Nov;46(11):5997-6015. doi: 10.21873/anticanres.18433. PMID- 42946436 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - Definitive Chemoradiotherapy Following Preoperative Induction Chemotherapy in Esophageal Squamous Cell Carcinoma: Prognostic Impact of Response. PG - 6235-6248 LID - 10.21873/anticanres.18453 [doi] AB - BACKGROUND/AIM: The role of induction chemotherapy (IC) before definitive chemoradiotherapy (CRT) in esophageal squamous cell carcinoma (ESCC) remains controversial, and outcomes among patients who begin chemotherapy with preoperative intent but eventually transition to definitive CRT are not well characterized. This study aimed to describe outcomes of definitive CRT with and without prior IC and to explore the prognostic value of chemotherapy response. PATIENTS AND METHODS: Patients with clinical stage II-IVa ESCC treated with definitive radiotherapy were retrospectively reviewed at a single center between 2014 and 2022. Of them, 54 patients treated with definitive CRT were analyzed: 31 received preoperative IC before transitioning to definitive CRT, and 23 received upfront definitive CRT (non-IC group). The IC group was clinically heterogeneous, having transitioned to CRT for varied reasons (persistent unresectability in 18, patient preference in 9, comorbidity or perioperative risk in 4). Median follow-up was 22.5 months. RESULTS: Two-year overall survival (OS), progression-free survival (PFS), and locoregional control did not differ significantly between the IC and non-IC groups (OS: 65.4% vs. 59.4%, p=0.559; PFS: 33.7% vs. 52.0%, p=0.465; locoregional control: 53.2% vs. 69.2%, p=0.402). Within the IC group, a response (partial/complete) to chemotherapy was associated with more favorable OS (p=0.008) and PFS (p=0.010); the association with locoregional control was not significant (p=0.059). Grade >/=3 late adverse events were persistent in the IC group, and there were four treatment-related deaths (7%). CONCLUSION: Induction chemotherapy before definitive CRT was not associated with improved survival in this selected cohort of ESCC. Response to induction chemotherapy was associated with more favorable OS and PFS and may have prognostic relevance, although it does not justify a response-adapted treatment strategy. CI - Copyright (c) 2026 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Hisano, Osamu AU - Hisano O AD - Department of Clinical Radiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan; hisano.osamu.618@m.kyushu-u.ac.jp. FAU - Yoshitake, Tadamasa AU - Yoshitake T AD - Department of Clinical Radiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. FAU - Matsumoto, Keiji AU - Matsumoto K AD - Department of Clinical Radiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. FAU - Shirakawa, Yuko AU - Shirakawa Y AD - Department of Clinical Radiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. FAU - Wakiyama, Hiroaki AU - Wakiyama H AD - Department of Clinical Radiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. FAU - Takaki, Masanori AU - Takaki M AD - Department of Clinical Radiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. FAU - Imafuku, Hikaru AU - Imafuku H AD - Department of Clinical Radiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. FAU - Tsuchihashi, Kenji AU - Tsuchihashi K AD - Department of Hematology, Oncology and Cardiovascular Medicine, Kyushu University Hospital, Fukuoka, Japan. FAU - Nakanoko, Tomonori AU - Nakanoko T AD - Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. FAU - Horioka, Kohei AU - Horioka K AD - Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. FAU - Matsumoto, Sokichi AU - Matsumoto S AD - Department of Surgery and Oncology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. FAU - Ishigami, Kousei AU - Ishigami K AD - Department of Clinical Radiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. LA - eng PT - Journal Article PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 SB - IM MH - Humans MH - *Induction Chemotherapy/methods MH - Prognosis MH - Female MH - *Esophageal Squamous Cell Carcinoma/therapy/mortality/pathology/drug therapy MH - *Chemoradiotherapy/methods MH - Male MH - *Esophageal Neoplasms/therapy/pathology/mortality/drug therapy MH - Middle Aged MH - Retrospective Studies MH - Aged MH - Treatment Outcome MH - Neoadjuvant Therapy OTO - NOTNLM OT - Esophageal squamous cell carcinoma OT - definitive chemoradiotherapy OT - preoperative-induction chemotherapy OT - prognosis OT - treatment response EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:04 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:04 [entrez] AID - 46/11/6235 [pii] AID - 10.21873/anticanres.18453 [doi] PST - ppublish SO - Anticancer Res. 2026 Nov;46(11):6235-6248. doi: 10.21873/anticanres.18453. PMID- 42946431 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - Treatment-specific Association of H&E-based Stromal Tumor-infiltrating Lymphocytes With Survival in Pleural Mesothelioma Treated With Ipilimumab/Nivolumab or Chemotherapy. PG - 6193-6202 LID - 10.21873/anticanres.18449 [doi] AB - BACKGROUND/AIM: Pleural mesothelioma lacks robust biomarkers to guide personalized treatment selection. Although stromal tumor-infiltrating lymphocytes (sTILs) on hematoxylin and eosin (H&E)-stained sections represent a cost-effective biomarker in various cancers, their clinical significance in pleural mesothelioma remains unclear. This study evaluated the prognostic impact of sTILs in pleural mesothelioma patients treated with platinum-pemetrexed chemotherapy or dual immune checkpoint inhibition (ICI) using ipilimumab plus nivolumab (Ipi/Nivo). PATIENTS AND METHODS: We retrospectively analyzed 179 patients with unresectable pleural mesothelioma who received either platinum-pemetrexed chemotherapy (n=127) or Ipi/Nivo (n=52). sTILs were assessed according to the International TILs Working Group guidelines and categorized as high (>10%) or low (5 cm posed persistent risks; age 61-70, >/=71 years, and micropapillary histology showed an early high risk that attenuated. Endocrine therapy's protective effect waned. For IDC, age 51-60 years was a persistent risk factor; hormone receptor positivity, N1 lymph node metastasis, and chemotherapy showed persistent inverse associations with CIBC risk. Age 61-70, >/=71 years, lymph nodal metastasis stages 2-3 (N2/N3), and tumor with size >5 cm exhibited early high risk with later attenuation. Notably, as observed in the curves, Black patients appeared to show higher peak risks for a subset of risk factors but shorter high-risk periods versus White patients. CONCLUSION: Under the competing risk of death, some risk factors for CIBC after DCIS and IDC exhibit significant time-varying effects, with potential differences between Black and White patients. These findings may help develop individualized screening strategies and reduce the use of contralateral prophylactic mastectomy (CPM). CI - Copyright (c) 2026 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Guo, Yixin AU - Guo Y AD - Breast Disease Diagnosis and Treatment Center of Affiliated Hospital of Qinghai University & Affiliated Cancer Hospital of Qinghai University, Xining, P.R. China. FAU - Li, Yongxin AU - Li Y AD - Breast Disease Diagnosis and Treatment Center of Affiliated Hospital of Qinghai University & Affiliated Cancer Hospital of Qinghai University, Xining, P.R. China. FAU - Xiao, Xinyu AU - Xiao X AD - College of Medical Information and Artificial Intelligence, Shandong First Medical University, Taian, P.R. China. FAU - Song, Ziqian AU - Song Z AD - College of Pharmacy, Qinghai University, Chengbei District, Xining, P.R. China. FAU - Li, Xuan AU - Li X AD - Jining Medical University, Affiliated Hospital of Jining Medical University, Jining, P.R. China. FAU - Zhao, Xiyue AU - Zhao X AD - Ordos Clinical Medical School of Inner Mongolia Medical University, Ordos, P.R. China. FAU - Guo, Shanchun AU - Guo S AD - RCMI Cancer Research Center and Department of Chemistry, Xavier University of Louisiana, New Orleans, LA, U.S.A. FAU - Zhao, Jiuda AU - Zhao J AD - Breast Disease Diagnosis and Treatment Center of Affiliated Hospital of Qinghai University & Affiliated Cancer Hospital of Qinghai University, Xining, P.R. China; jiudazhao@126.com. LA - eng PT - Journal Article PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 SB - IM MH - Aged MH - Female MH - Humans MH - Middle Aged MH - *Black or African American/statistics & numerical data MH - *Breast Neoplasms/pathology/epidemiology/ethnology MH - *Carcinoma, Ductal, Breast/pathology/epidemiology MH - *Carcinoma, Intraductal, Noninfiltrating/pathology/epidemiology MH - Risk Factors MH - SEER Program MH - Time Factors MH - *White/statistics & numerical data OTO - NOTNLM OT - Contralateral invasive breast cancer (CIBC) OT - overtreatment OT - racial disparities OT - risk factors OT - time-varying effects EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:04 PHST- 2026/07/03 00:00 [received] PHST- 2026/07/17 00:00 [revised] PHST- 2026/07/21 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:04 [entrez] AID - 46/11/6437 [pii] AID - 10.21873/anticanres.18470 [doi] PST - ppublish SO - Anticancer Res. 2026 Nov;46(11):6437-6450. doi: 10.21873/anticanres.18470. PMID- 42946422 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - Impact of BMI on Perioperative Complications Following CRS and HIPEC for Appendiceal Cancer. PG - 6219-6224 LID - 10.21873/anticanres.18451 [doi] AB - BACKGROUND/AIM: Obesity is considered a risk factor for poorer postoperative outcomes. Its effect in the context of cytoreductive surgery (CRS) and heated intraperitoneal chemotherapy (HIPEC) is less well understood. This study examined the effect of body mass index (BMI) on postoperative surgical outcomes in patients with appendiceal cancer who underwent CRS/HIPEC. PATIENTS AND METHODS: A retrospective analysis performed including adult patients with appendiceal cancer who underwent CRS/HIPEC between 2021 to 2023 and grouped into weight ranges based on body mass index (BMI). Intraoperative and postoperative outcomes were recorded. Continuous variables were compared using Kruskal Wallis tests while categorical variables were compared using Chi squared tests. Multivariate regression analysis was performed and models controlled for age, sex and peritoneal cancer index. RESULTS: A total of 130 adult patients who underwent CRS/HIPEC for appendiceal cancer were included in the analysis. Intraoperative outcomes were not significantly different between weight range groups. Wound complication rates were higher among patients with BMI >30 (p=0.014). The mortality rate and rate of complications were not statistically different between BMI groups. In the adjusted model, patients with higher BMI were more likely to develop cardiovascular complications [odds ratio (OR)=1.092, p=0.018] but were less likely to develop obstruction or ileus postoperatively (OR=0.911, p=0.045). CONCLUSION: Surgery is a key component of the management of peritoneal surface malignancies but patients with higher BMI may be at increased risk of perioperative complications, potentially altering the perioperative management of this patient cohort. However, it is unclear what specific risks obesity confers to morbidity and mortality or oncological outcomes. In our study, when stratified by BMI, there was a significant difference in wound-related complications and increased likelihood of cardiovascular complications in patients with higher BMI who underwent CRS/HIPEC for appendiceal malignancy. There were no other significant differences in intra-operative or morbidity outcomes. These findings would suggest that high BMI should be considered but not prohibit patient selection for operative management. In such patients, BMI and cardiovascular risk profile should be optimised to improve perioperative outcomes. CI - Copyright (c) 2026 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Mercado, Melanie AU - Mercado M AD - Peritonectomy Unit, Department of Surgery, St George Hospital, Kogarah, NSW, Australia; mel.cado26@gmail.com. AD - St George and Sutherland Clinical School, University of New South Wales, Sydney, NSW, Australia. FAU - Vaska, Ashish I AU - Vaska AI AD - Peritonectomy Unit, Department of Surgery, St George Hospital, Kogarah, NSW, Australia. FAU - Barat, Shoma AU - Barat S AD - Peritonectomy Unit, Department of Surgery, St George Hospital, Kogarah, NSW, Australia. FAU - Wijayawardana, Ruwanthi AU - Wijayawardana R AD - Peritonectomy Unit, Department of Surgery, St George Hospital, Kogarah, NSW, Australia. FAU - Ahmadi, Nima AU - Ahmadi N AD - Peritonectomy Unit, Department of Surgery, St George Hospital, Kogarah, NSW, Australia. FAU - Morris, David L AU - Morris DL AD - Peritonectomy Unit, Department of Surgery, St George Hospital, Kogarah, NSW, Australia. AD - St George and Sutherland Clinical School, University of New South Wales, Sydney, NSW, Australia. LA - eng PT - Journal Article PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 SB - IM MH - Humans MH - Female MH - Male MH - *Appendiceal Neoplasms/therapy/surgery MH - *Body Mass Index MH - Middle Aged MH - *Cytoreduction Surgical Procedures/adverse effects MH - Retrospective Studies MH - *Postoperative Complications/etiology/epidemiology MH - *Hyperthermic Intraperitoneal Chemotherapy/adverse effects MH - Adult MH - *Obesity/complications MH - Aged MH - Risk Factors MH - Hyperthermia, Induced OTO - NOTNLM OT - Appendiceal cancer OT - BMI OT - CRS/HIPEC OT - perioperative complications EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:04 PHST- 2026/06/02 00:00 [received] PHST- 2026/09/02 00:00 [revised] PHST- 2026/09/03 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:04 [entrez] AID - 46/11/6219 [pii] AID - 10.21873/anticanres.18451 [doi] PST - ppublish SO - Anticancer Res. 2026 Nov;46(11):6219-6224. doi: 10.21873/anticanres.18451. PMID- 42946421 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - Preoperative Albumin-derived Neutrophil-to-Lymphocyte Ratio Predicts Survival Across Pathological Stages of Colorectal Cancer. PG - 6417-6425 LID - 10.21873/anticanres.18468 [doi] AB - BACKGROUND/AIM: The albumin-derived neutrophil-to-lymphocyte ratio (Alb-dNLR), reflecting nutritional and inflammatory status, has been reported as a prognostic marker in malignancies. However, its significance across all pathological stages of colorectal cancer remains unclear. This study evaluated preoperative Alb-dNLR in colorectal cancer surgery. PATIENTS AND METHODS: We retrospectively analyzed 220 patients who underwent colorectal cancer resection at Tochigi Medical Center Shimotsuga. Alb-dNLR was calculated as serum albumin divided by the derived neutrophil-to-lymphocyte ratio. Patients were classified into high and low groups using a receiver operating characteristic-derived cutoff. Overall survival (OS) and clinicopathological characteristics were analyzed. RESULTS: The optimal Alb-dNLR cutoff was 1.395. Low Alb-dNLR was associated with deeper tumor invasion, lymph node metastasis, and advanced stage. Logistic regression identified pT3-4 as an independent factor related to low Alb-dNLR (OR=6.776; 95% CI=2.310-25.501; p=0.0002). Low Alb-dNLR was associated with worse OS than high Alb-dNLR (5-year OS, 53.3% vs. 76.2%; p<0.001). This association was consistent across pathological stages. Low Alb-dNLR independently predicted poor OS (HR=3.359; 95% CI=1.89-6.81; p<0.0001). CONCLUSION: Preoperative Alb-dNLR is an independent prognostic marker associated with tumor progression and poor survival in colorectal cancer, with consistent prognostic value across pathological stages. CI - Copyright (c) 2026 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Omameuda, Takahiko AU - Omameuda T AD - Department of Surgery, Tochigi Medical Center Shimotsuga, Tochigi, Japan. FAU - Miki, Atsushi AU - Miki A AD - Department of Surgery, Tochigi Medical Center Shimotsuga, Tochigi, Japan; amiki@jichi.ac.jp. AD - Department of Surgery, Division of Gastroenterological, General and Transplant Surgery, Jichi Medical University, Shimotsuke, Japan. AD - Division of Cell Biology and Anatomy, Jichi Medical University, Shimotsuke, Japan. FAU - Sakuma, Yasunaru AU - Sakuma Y AD - Department of Surgery, Division of Gastroenterological, General and Transplant Surgery, Jichi Medical University, Shimotsuke, Japan. FAU - Yamaguchi, Hironori AU - Yamaguchi H AD - Department of Surgery, Division of Gastroenterological, General and Transplant Surgery, Jichi Medical University, Shimotsuke, Japan. FAU - Nishimura, Wataru AU - Nishimura W AD - Division of Cell Biology and Anatomy, Jichi Medical University, Shimotsuke, Japan. FAU - Koizumi, Masaru AU - Koizumi M AD - Department of Surgery, Tochigi Medical Center Shimotsuga, Tochigi, Japan. LA - eng PT - Journal Article PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 RN - 0 (Serum Albumin) SB - IM MH - Humans MH - *Colorectal Neoplasms/pathology/blood/surgery/mortality MH - Female MH - *Neutrophils/pathology MH - Male MH - *Lymphocytes/pathology MH - Aged MH - Prognosis MH - Neoplasm Staging MH - Retrospective Studies MH - Middle Aged MH - *Serum Albumin/analysis/metabolism MH - Preoperative Period MH - Aged, 80 and over MH - Lymphatic Metastasis OTO - NOTNLM OT - Albumin-derived neutrophil-to-lymphocyte ratio OT - colorectal cancer OT - prognosis EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:04 PHST- 2026/07/14 00:00 [received] PHST- 2026/08/09 00:00 [revised] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:04 [entrez] AID - 46/11/6417 [pii] AID - 10.21873/anticanres.18468 [doi] PST - ppublish SO - Anticancer Res. 2026 Nov;46(11):6417-6425. doi: 10.21873/anticanres.18468. PMID- 42946420 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - Abscopal-like Regression of Lung Metastases After Near-infrared Photoimmunotherapy Plus Nivolumab in Laryngeal Carcinoma. PG - 6457-6465 LID - 10.21873/anticanres.18472 [doi] AB - BACKGROUND/AIM: Near-infrared photoimmunotherapy (NIR-PIT) combines tumor-selective cytotoxicity with the induction of immunogenic cell death and may therefore augment the systemic activity of immune checkpoint inhibitors (ICIs). However, clinical evidence of responses in distant, non-illuminated lesions is lacking. We describe a distant visceral response following NIR-PIT administered during programmed cell death protein 1 (PD-1) blockade. CASE REPORT: A man in his 60s presented with recurrent laryngeal squamous cell carcinoma involving the base of the tongue and multiple pulmonary metastases that had progressed after definitive chemoradiotherapy, salvage total laryngectomy, re-irradiation, and platinum- and pembrolizumab-containing regimens. Nivolumab monotherapy was initiated, and NIR-PIT of the base-of-tongue lesion was added one month later. Using the pre-NIR-PIT status as baseline, the target pulmonary metastasis decreased from 12.1 to 8.0 mm and a cervical lymph node from 15.3 to 11.1 mm at 4 months (sum -30.3%; partial response by RECIST 1.1), with both lesions located outside the illuminated field. This response deepened and was maintained at 10 months (7.6 and 8.2 mm; sum -42.3%). In contrast, the illuminated base-of-tongue lesion persisted despite a second NIR-PIT at 5 months. Adverse events were limited to grade 2 facial and cervical edema and local pain. CONCLUSION: The addition of local NIR-PIT to nivolumab was followed by durable, abscopal-like regression of non-illuminated pulmonary metastases in immunotherapy-refractory head and neck cancer. While causality cannot be established from a single case, this observation supports formal investigation of NIR-PIT-ICI combinations in patients with distant metastases. CI - Copyright (c) 2026 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Ishida, Yoshiya AU - Ishida Y AD - Department of Otolaryngology - Head and Neck Surgery, Japanese Red Cross Kitami Hospital, Kitami, Japan; yoshiyaishida@icloud.com. FAU - Yuasa, Ryota AU - Yuasa R AD - Department of Otolaryngology - Head and Neck Surgery, Japanese Red Cross Kitami Hospital, Kitami, Japan. AD - Department of Otolaryngology - Head and Neck Surgery, Asahikawa Medical University, Asahikawa, Japan. FAU - Nakamuta, Koki AU - Nakamuta K AD - Department of Otolaryngology - Head and Neck Surgery, Japanese Red Cross Kitami Hospital, Kitami, Japan. AD - Department of Otolaryngology - Head and Neck Surgery, Asahikawa Medical University, Asahikawa, Japan. FAU - Wada, Tetsuji AU - Wada T AD - Department of Otolaryngology - Head and Neck Surgery, Japanese Red Cross Kitami Hospital, Kitami, Japan. LA - eng PT - Case Reports PT - Journal Article PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 RN - 31YO63LBSN (Nivolumab) RN - 0 (Antineoplastic Agents, Immunological) SB - IM MH - Humans MH - Male MH - *Nivolumab/therapeutic use MH - *Lung Neoplasms/secondary/therapy/drug therapy MH - *Laryngeal Neoplasms/pathology/therapy MH - *Immunotherapy/methods MH - Middle Aged MH - *Phototherapy/methods MH - *Antineoplastic Agents, Immunological/therapeutic use MH - Combined Modality Therapy OTO - NOTNLM OT - Near-infrared photoimmunotherapy OT - abscopal effect OT - case report OT - immune checkpoint inhibitor OT - immunogenic cell death OT - recurrent laryngeal squamous cell carcinoma EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:04 PHST- 2026/09/01 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:04 [entrez] AID - 46/11/6457 [pii] AID - 10.21873/anticanres.18472 [doi] PST - ppublish SO - Anticancer Res. 2026 Nov;46(11):6457-6465. doi: 10.21873/anticanres.18472. PMID- 42946419 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - Overall Survival After Neoadjuvant Versus Adjuvant Chemotherapy for Invasive Urothelial Carcinoma of the Bladder: A Real-world Multicenter Cohort Study. PG - 6331-6341 LID - 10.21873/anticanres.18461 [doi] AB - BACKGROUND/AIM: Invasive urothelial carcinoma of the urinary bladder carries a high risk of micrometastatic spread, and perioperative chemotherapy is a key component of curative-intent treatment. Neoadjuvant chemotherapy (NAC) has the strongest supporting evidence, whereas the role of adjuvant chemotherapy (AC) remains debated and real-world comparisons are limited. We compared overall survival (OS) and the development of liver metastasis between patients treated with NAC and those treated with AC who underwent radical cystectomy. PATIENTS AND METHODS: Using the TriNetX U.S. Collaborative Network, we identified adults with bladder cancer who underwent radical cystectomy and received cisplatin or carboplatin within 1 year before (NAC) or within 1 year after (AC) surgery. The two cohorts were balanced by 1:1 propensity score-matching for age, sex, and race. OS (primary endpoint) and time to liver metastasis (secondary endpoint) were estimated by the Kaplan-Meier method and compared with the log-rank test. RESULTS: After matching, 402 patients were included in each group. Median OS was 2,136 days for the AC group and was not reached for the NAC group. NAC was associated with significantly longer OS than AC (hazard ratio=0.528, 95% confidence interval=0.413-0.676, p<0.0001). Liver metastasis developed in 30 (7.5%) patients in the NAC group and 93 (23.1%) in the AC group, and NAC was associated with a lower risk of liver metastasis (hazard ratio=0.284, 95% confidence interval=0.188-0.428, p<0.0001). CONCLUSION: In this real-world cohort, NAC was associated with significantly longer OS and a lower risk of liver metastasis than AC in patients undergoing radical cystectomy for invasive bladder cancer. These hypothesis-generating findings support the preferential use of NAC when feasible and warrant prospective validation. CI - Copyright (c) 2026 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Chen, Hao-Wei AU - Chen HW AD - Department of Urology, Taichung Veterans General Hospital, Taichung, Taiwan, R.O.C. FAU - Li, Jian-Ri AU - Li JR AD - Department of Urology, Taichung Veterans General Hospital, Taichung, Taiwan, R.O.C. AD - Department of Post-Baccalaureate Medicine, College of Medicine, National Chung Hsing University, Taichung, Taiwan, R.O.C. AD - Department of Medicine and Nursing, Hung Kuang University, Taichung, Taiwan, R.O.C. FAU - Wang, Shian-Shiang AU - Wang SS AD - Department of Urology, Taichung Veterans General Hospital, Taichung, Taiwan, R.O.C. FAU - Yang, Cheng-Kuang AU - Yang CK AD - Department of Urology, Taichung Veterans General Hospital, Taichung, Taiwan, R.O.C. FAU - Hsu, Jan Yuan-Sheng AU - Hsu JY AD - Department of Urology, Taichung Veterans General Hospital, Taichung, Taiwan, R.O.C. FAU - Chang, Chia-Cheng AU - Chang CC AD - Department of Urology, Taichung Veterans General Hospital, Taichung, Taiwan, R.O.C.; changcc0830@vghtc.gov.tw. LA - eng PT - Comparative Study PT - Journal Article PT - Multicenter Study PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 RN - Q20Q21Q62J (Cisplatin) SB - IM MH - Aged MH - Female MH - Humans MH - Male MH - Middle Aged MH - *Carcinoma, Transitional Cell/drug therapy/mortality/secondary/surgery MH - Chemotherapy, Adjuvant MH - Cisplatin/therapeutic use/administration & dosage MH - Cohort Studies MH - Cystectomy MH - *Liver Neoplasms/epidemiology/prevention & control/secondary MH - *Neoadjuvant Therapy MH - Neoplasm Invasiveness MH - *Urinary Bladder Neoplasms/drug therapy/pathology/mortality/surgery OTO - NOTNLM OT - Urinary bladder neoplasms OT - adjuvant chemotherapy OT - neoadjuvant chemotherapy OT - overall survival OT - propensity score OT - real-world evidence EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:04 PHST- 2026/07/12 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:04 [entrez] AID - 46/11/6331 [pii] AID - 10.21873/anticanres.18461 [doi] PST - ppublish SO - Anticancer Res. 2026 Nov;46(11):6331-6341. doi: 10.21873/anticanres.18461. PMID- 42946418 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - Epidermal Growth Factor Receptor Signaling and Crosstalk With Aspartate-beta-Hydroxylase Modulate Chondrosarcoma Cell Growth and Invasive Potential. PG - 5967-5996 LID - 10.21873/anticanres.18432 [doi] AB - BACKGROUND/AIM: Aspartate beta-Hydroxylase (ASPH) is an oncofetal protein overexpressed in malignancies, including chondrosarcoma. Although its functional roles in malignant neoplastic cell motility and invasiveness have been linked to hydroxylation of epidermal growth factor (EGF)-like domains expressed in molecules such as Notch and Jagged, its relationship to EGF receptor (EGFR) signaling has not been investigated. This study utilized CS-1 (high grade) and CDS-11 (intermediate grade) human chondrosarcoma cell lines to characterize the effects of EGFR stimulation on ASPH expression and function. MATERIALS AND METHODS: CS-1 and CDS11 cells were treated with the trophic factors EGF, TGF-alpha, TGF-beta, IGF-1, or vehicle (0.5% FBS) and assayed for growth, viability, morphology, motility, invasiveness, EGFR activation, Notch pathway gene expression, and ASPH expression. RESULTS: EGF, TGF-alpha, and TGF-beta increased the expression of EGFR, proliferating cell nuclear antigen (PCNA), and ASPH-FB50, EGF and TGF-beta stimulated tyrosine-phosphorylated (pY) EGFR, and EGF and TGF-alpha stimulated vimentin in the CDS11 but not CS-1 cells. The stimulatory effects of EGF, TGF-alpha, and TGF-beta were associated with significantly increased directional motility and invasion. Further studies suggested that the effects of EGFR pathway stimulation by TGF-beta may be mediated by differential increases in the expression of IGF1R, IRS1, JAGGED, and HES or HEY NOTCH transcription factors. CONCLUSION: The results of these investigations suggest important roles for EGF, TGF-alpha, and TGF-beta regulation of growth, motility, invasiveness, and ASPH-FB50 immunoreactivity via EGFR in CDS11 chondrosarcoma cells. In addition, CDS11 cellular responses to TGF-beta may have been mediated by increased ASPH protein expression together with IGF/IRS and Notch network crosstalk. Additional therapeutic strategies for chondrosarcoma may include EGFR and ASPH inhibitors in combination with conventional chemotherapy. CI - Copyright (c) 2026 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Oyama, Keisuke AU - Oyama K AD - Department of Orthopedic Surgery, Rhode Island Hospital, Alpert Medical School at Brown University, Providence, RI, U.S.A. AD - Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka, Japan. FAU - Tong, Ming AU - Tong M AD - Department of Medicine, Rhode Island Hospital, Brown University Health, Alpert Medical School at Brown University, Providence, RI, U.S.A. FAU - Li, Zhengliang AU - Li Z AD - Biotechnology Masters Degree Program, Brown University, Providence, RI, U.S.A. FAU - Carlson, Rolf I AU - Carlson RI AD - Department of Medicine, Rhode Island Hospital, Brown University Health, Alpert Medical School at Brown University, Providence, RI, U.S.A. FAU - Rodriguez, Rene AU - Rodriguez R AD - Health Research Institute of Asturias (ISPA), University Institute of Oncology of Asturias (IUOPA), Oviedo, Spain. AD - CIBER en oncologia (CIBERONC), Madrid, Spain. AD - Nanomaterials and Nanotechnology Research Center (CINN-CSIC), Universidad de Oviedo, Principado de Asturias, El Entrego, Spain. FAU - DE LA Monte, Suzanne M AU - DE LA Monte SM AD - Departments of Pathology and Laboratory Medicine, Neurology, and Neurosurgery, Rhode Island Hospital, Women & Infants Hospital, Providence, RI, U.S.A.; suzanne_delamonte_md@brown.edu. AD - Brown University Health, and the Alpert Medical School at Brown University, Rhode Island, Providence, RI, U.S.A. LA - eng PT - Journal Article PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 RN - EC 2.7.10.1 (ErbB Receptors) RN - EC 2.7.10.1 (EGFR protein, human) RN - EC 1.- (Mixed Function Oxygenases) RN - EC 1.14.11.16 (ASPH protein, human) RN - 0 (Receptors, Notch) RN - 0 (Calcium-Binding Proteins) RN - 0 (Transforming Growth Factor beta) RN - 67763-96-6 (Insulin-Like Growth Factor I) RN - 0 (Membrane Proteins) RN - 0 (Transforming Growth Factor alpha) RN - 62229-50-9 (Epidermal Growth Factor) RN - 0 (Muscle Proteins) SB - IM MH - Humans MH - *ErbB Receptors/metabolism/genetics MH - *Chondrosarcoma/pathology/metabolism/genetics MH - Signal Transduction MH - Cell Line, Tumor MH - Neoplasm Invasiveness MH - Cell Proliferation/drug effects MH - Cell Movement/drug effects MH - *Mixed Function Oxygenases/metabolism/genetics MH - *Bone Neoplasms/pathology/metabolism/genetics MH - Receptors, Notch/metabolism MH - *Calcium-Binding Proteins/metabolism/genetics MH - Transforming Growth Factor beta/pharmacology MH - Insulin-Like Growth Factor I/pharmacology MH - Gene Expression Regulation, Neoplastic/drug effects MH - *Membrane Proteins/metabolism/genetics MH - Transforming Growth Factor alpha/pharmacology MH - Epidermal Growth Factor/pharmacology MH - Muscle Proteins OTO - NOTNLM OT - Aspartate-beta-hydroxylase OT - EGFR OT - Notch OT - chondrosarcoma OT - transforming growth factor OT - tumor invasion EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:04 PHST- 2026/04/13 00:00 [received] PHST- 2026/09/03 00:00 [revised] PHST- 2026/09/07 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:04 [entrez] AID - 46/11/5967 [pii] AID - 10.21873/anticanres.18432 [doi] PST - ppublish SO - Anticancer Res. 2026 Nov;46(11):5967-5996. doi: 10.21873/anticanres.18432. PMID- 42946413 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - Association of Institution-wide Fluorescent Ureteral Navigation Implementation With Minimally Invasive Surgery Completion in Rectal Cancer. PG - 6203-6217 LID - 10.21873/anticanres.18450 [doi] AB - BACKGROUND/AIM: Fluorescent ureteral navigation (FUN) enables real-time ureter visualization during pelvic surgery, but whether institution-wide implementation supports minimally invasive surgery (MIS) is unclear. PATIENTS AND METHODS: We retrospectively compared consecutive rectal and anal canal cancer operations before (January 2017-January 2020; n=122) and after (February 2020-August 2025; n=289) implementation of a standardized catheter-based FUN workflow. After implementation, FUN was planned in 98 patients (33.9%) and used as intraoperative rescue in 3 additional patients. RESULTS: Propensity score matching yielded 117 pairs. Among 106 matched pairs in which MIS was initiated in both eras, conversion to open surgery occurred in 7 patients (6.6%) before implementation and none after implementation (p=0.016). MIS completion among all matched cases increased from 84.6% to 100% (p<0.001). Major complications were similar (11.1% vs. 10.3%). Exploratory Firth-penalized logistic regression yielded an adjusted odds ratio of 41.4 for MIS completion in the postimplementation era (95% CI=2.78-617.41; p=0.007). CONCLUSION: Institution-wide FUN implementation was associated with higher MIS completion without an apparent increase in major complications despite an increasingly complex case mix. CI - Copyright (c) 2026 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Ryu, Shunjin AU - Ryu S AD - Department of Digestive Surgery, Kawaguchi Municipal Medical Center, Saitama, Japan; systematic.ryu1121@gmail.com. FAU - Imaizumi, Yuta AU - Imaizumi Y AD - Department of Digestive Surgery, Kawaguchi Municipal Medical Center, Saitama, Japan. FAU - Nakashima, Shunsuke AU - Nakashima S AD - Department of Digestive Surgery, Kawaguchi Municipal Medical Center, Saitama, Japan. FAU - Kawakubo, Hyuga AU - Kawakubo H AD - Department of Digestive Surgery, Kawaguchi Municipal Medical Center, Saitama, Japan. FAU - Suzuki, Daiki AU - Suzuki D AD - Department of Digestive Surgery, Kawaguchi Municipal Medical Center, Saitama, Japan. FAU - Goto, Keisuke AU - Goto K AD - Department of Surgery, The Jikei University School of Medicine, Tokyo, Japan. FAU - Kamada, Teppei AU - Kamada T AD - Department of Surgery, The Jikei University School of Medicine, Tokyo, Japan. FAU - Takano, Yasuhiro AU - Takano Y AD - Department of Surgery, The Jikei University School of Medicine, Tokyo, Japan. FAU - Eto, Ken AU - Eto K AD - Department of Surgery, The Jikei University School of Medicine, Tokyo, Japan. LA - eng PT - Journal Article PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 SB - IM MH - Humans MH - Female MH - Male MH - *Minimally Invasive Surgical Procedures/methods MH - Retrospective Studies MH - Middle Aged MH - Aged MH - *Rectal Neoplasms/surgery/diagnostic imaging MH - *Ureter/surgery/diagnostic imaging MH - Colorectal Surgical Procedures OTO - NOTNLM OT - Colorectal cancer OT - fluorescence OT - minimally invasive surgery OT - surgical navigation OT - ureteral navigation EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:04 PHST- 2026/09/05 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:04 [entrez] AID - 46/11/6203 [pii] AID - 10.21873/anticanres.18450 [doi] PST - ppublish SO - Anticancer Res. 2026 Nov;46(11):6203-6217. doi: 10.21873/anticanres.18450. PMID- 42946412 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - Baseline Serum Albumin Is Associated With Severe Adverse Events During Trastuzumab Deruxtecan Treatment in Metastatic Breast Cancer. PG - 6183-6192 LID - 10.21873/anticanres.18448 [doi] AB - BACKGROUND/AIM: Trastuzumab deruxtecan (T-DXd) is associated with a high incidence of severe adverse events (AEs); however, clinically useful baseline predictors of severe AEs are limited. This study aimed to explore factors associated with severe AEs in patients with metastatic breast cancer receiving T-DXd using least absolute shrinkage and selection operator (LASSO) regression. PATIENTS AND METHODS: This single-center retrospective study included 31 patients with HER2-positive or HER2-low metastatic breast cancer who received T-DXd. Severe AEs were defined as grade >/=3 AEs. LASSO logistic regression was used to select candidate factors from 21 clinical and laboratory variables. Variables selected at lambda1se were entered into multivariable logistic regression, and the optimal cutoff value was estimated using receiver operating characteristic (ROC) curve analysis. RESULTS: Severe AEs occurred in 11 patients (35.5%). LASSO regression selected serum albumin and hemoglobin as candidate variables. In multivariable analysis, serum albumin was independently associated with severe AEs [odds ratio (OR)=0.065; 95% confidence interval (CI)=0.006-0.717; p<0.05], whereas hemoglobin was not statistically significant (p=0.07). ROC analysis identified a cutoff of 3.5 g/dl (area under the curve, 0.85; 95%CI=0.71-1.00; sensitivity, 91%; specificity, 75%). Severe AEs occurred more frequently in patients with serum albumin 3.5 g/dl (66.7% vs. 6.2%; p<0.05). CONCLUSION: Lower baseline serum albumin may help identify patients at increased risk of severe AEs during T-DXd treatment for metastatic breast cancer. CI - Copyright (c) 2026 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Imai, Yuri AU - Imai Y AD - Faculty of Pharmacy, Nagoya City University Hospital, Nagoya, Japan. FAU - Mimura, Yoshihisa AU - Mimura Y AD - Department of Clinical Pharmaceutics, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. FAU - Iida, Moeko AU - Iida M AD - Faculty of Pharmacy, Nagoya City University Hospital, Nagoya, Japan. AD - Department of Clinical Pharmaceutics, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. FAU - Ito, Nanami AU - Ito N AD - Faculty of Pharmacy, Nagoya City University Hospital, Nagoya, Japan. AD - Department of Clinical Pharmaceutics, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. FAU - Oyama, Yui AU - Oyama Y AD - Faculty of Pharmacy, Nagoya City University Hospital, Nagoya, Japan. FAU - Odagiri, Kunihiro AU - Odagiri K AD - Faculty of Pharmacy, Nagoya City University Hospital, Nagoya, Japan. AD - Department of Clinical Pharmaceutics, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. FAU - Tasaki, Yoshihiko AU - Tasaki Y AD - Faculty of Pharmacy, Nagoya City University Hospital, Nagoya, Japan. AD - Department of Clinical Pharmaceutics, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. FAU - Sugiyama, Yosuke AU - Sugiyama Y AD - Faculty of Pharmacy, Nagoya City University Hospital, Nagoya, Japan. AD - Department of Clinical Pharmaceutics, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. FAU - Wanifuchi-Endo, Yumi AU - Wanifuchi-Endo Y AD - Department of Breast Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. FAU - Hotta, Yuji AU - Hotta Y AD - Faculty of Pharmacy, Nagoya City University Hospital, Nagoya, Japan. AD - Department of Clinical Pharmaceutics, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. FAU - Toyama, Tatsuya AU - Toyama T AD - Department of Breast Surgery, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. FAU - Furukawa-Hibi, Yoko AU - Furukawa-Hibi Y AD - Faculty of Pharmacy, Nagoya City University Hospital, Nagoya, Japan; yokoh@med.nagoya-cu.ac.jp. AD - Department of Clinical Pharmaceutics, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan. LA - eng PT - Journal Article PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 RN - P188ANX8CK (Trastuzumab) RN - 0 (Serum Albumin) SB - IM MH - Humans MH - *Breast Neoplasms/drug therapy/pathology/blood MH - Female MH - *Trastuzumab/adverse effects/therapeutic use/administration & dosage MH - Retrospective Studies MH - Middle Aged MH - *Serum Albumin/metabolism/analysis MH - Neoplasm Metastasis MH - Aged MH - Adult MH - ROC Curve OTO - NOTNLM OT - LASSO regression OT - Trastuzumab deruxtecan OT - adverse events OT - serum albumin EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:04 PHST- 2026/09/04 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:04 [entrez] AID - 46/11/6183 [pii] AID - 10.21873/anticanres.18448 [doi] PST - ppublish SO - Anticancer Res. 2026 Nov;46(11):6183-6192. doi: 10.21873/anticanres.18448. PMID- 42946409 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - Circulating and Soluble Biomarkers for Predicting Inguinal Lymph Node Metastasis in Penile Squamous Cell Carcinoma: A Scoping Review. PG - 5933-5945 LID - 10.21873/anticanres.18429 [doi] AB - BACKGROUND/AIM: Regional lymph node status is the strongest prognostic determinant in penile squamous cell carcinoma (PSCC), yet current staging relies on invasive procedures with substantial morbidity. Numerous circulating and soluble biomarkers have been proposed to non-invasively predict lymph node metastasis (LNM), but this body of evidence has never been systematically mapped. This scoping review aimed to chart the extent, nature and maturity of the evidence on circulating and soluble biomarkers for LNM prediction in PSCC. MATERIALS AND METHODS: A scoping review was conducted following the Joanna Briggs Institute methodology and reported according to the PRISMA Extension for Scoping Reviews (PRISMA-ScR). Bibliographic sources indexed in PubMed/MEDLINE, Embase, Scopus and Google Scholar were searched up to July 2026 using terms combining "penile neoplasms", "lymph node metastasis" and "biomarker/soluble/circulating/immune checkpoint". Studies reporting an association between a blood-derived biomarker and nodal status in PSCC were charted narratively by biomarker class. Tissue-based checkpoint biomarkers were additionally included when necessary to provide biological context for their circulating counterparts. RESULTS: The evidence clusters into four principal classes of circulating/soluble biomarkers: (i) systemic inflammatory/nutritional markers (neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio, C-reactive protein), supported by dedicated meta-analyses and multiple retrospective cohorts; (ii) the tumor-associated antigen SCC-Ag, useful for monitoring but poorly sensitive for occult metastasis; (iii) immune-checkpoint-related proteins, mature at tissue level (PD-L1) but represented by a single dedicated study of soluble checkpoint proteins with weak external validation; and (iv) circulating nucleic acids (microRNA, cell-free/tumor DNA), essentially unexplored specifically for nodal prediction in PSCC despite validation in analogous squamous malignancies. HPV/p16 status, although prognostically informative, is tissue-based rather than circulating and was therefore charted separately as biological context rather than as a core circulating-biomarker class. CONCLUSION: Circulating biomarker research in PSCC remains fragmented and dominated by small, retrospective, single-center studies. Multi-marker panels, prospective multicenter validation leveraging rare-tumor consortia, and extension of liquid-biopsy approaches validated in other HPV-driven squamous cancers represent the most promising directions to reduce reliance on invasive nodal staging. CI - Copyright (c) 2026 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Dell'atti, Lucio AU - Dell'atti L AD - Department of Urology, University-Hospital of Marche, Ancona, Italy; dellatti@hotmail.com. FAU - Slyusar, Viktoria AU - Slyusar V AD - Pain Therapy Center, Division of Anesthesia and Intensive Care, University-Hospital of Marche, Ancona, Italy. LA - eng PT - Journal Article PT - Review PT - Scoping Review PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - *Penile Neoplasms/pathology/blood MH - *Lymphatic Metastasis MH - Male MH - *Biomarkers, Tumor/blood MH - *Carcinoma, Squamous Cell/blood/pathology MH - Prognosis MH - Inguinal Canal/pathology OTO - NOTNLM OT - C-reactive protein OT - Penile cancer OT - biomarker OT - immune checkpoint OT - liquid biopsy OT - lymph node metastasis OT - scoping review OT - squamous cell carcinoma EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:04 PHST- 2026/08/26 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:04 [entrez] AID - 46/11/5933 [pii] AID - 10.21873/anticanres.18429 [doi] PST - ppublish SO - Anticancer Res. 2026 Nov;46(11):5933-5945. doi: 10.21873/anticanres.18429. PMID- 42946407 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - The Tyro3 Inhibitor UNC9426 Induces Apoptosis in OEC-M1 OSCC Cells. PG - 6155-6168 LID - 10.21873/anticanres.18445 [doi] AB - BACKGROUND/AIM: Elevated Tyro3 expression has been associated with tumor cell survival and malignant progression, suggesting that Tyro3 may represent a therapeutic target in oral squamous cell carcinoma (OSCC). We evaluated the effects of the selective Tyro3 inhibitor UNC9426 in OEC-M1 OSCC cells and associated apoptosis-related signaling changes. MATERIALS AND METHODS: OEC-M1 cells and non-malignant HGF-1 gingival fibroblasts were used. Cell viability, apoptotic morphology, protein expression, caspase-cleaved cytokeratin-18 fragment release, and JC-1 fluorescence were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, light microscopy, immunoblotting, enzyme-linked immunosorbent assay, and JC-1 staining, respectively. Z-VAD-FMK, the Fas-blocking antibody ZB4, and BAX activation inhibitor 1 (BAI1) were used to examine caspase, Fas, and BAX involvement. RESULTS: OEC-M1 cells exhibited higher Tyro3 protein levels and a lower estimated UNC9426 half-maximal inhibitory concentration (IC(50)) than HGF-1 cells (1.375 vs. 3.987 muM). UNC9426 increased membrane blebbing, poly(ADP-ribose) polymerase cleavage, caspase-cleaved cytokeratin-18 fragment release, and cleavage of caspase-8, -9, -3, and -7. Qualitative JC-1 imaging showed an apparent increase in monomer-associated green fluorescence consistent with changes associated with mitochondrial membrane depolarization. Z-VAD-FMK attenuated UNC9426-induced cytokeratin-18 fragment release. UNC9426 reduced Tyro3 protein abundance and Akt and extracellular signal-regulated kinase (ERK) phosphorylation after 24 h and increased Fas and BAX protein levels after 48 h. ZB4 or BAI1 partially reversed the reduction in cell viability and attenuated cytokeratin-18 fragment release. CONCLUSION: UNC9426 induces apoptosis in OEC-M1 cells in association with reduced Tyro3 protein abundance and Akt/ERK phosphorylation, with Fas signaling and BAX activity contributing functionally to the apoptotic response. CI - Copyright (c) 2026 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Huang, Yaw-Chang AU - Huang YC AD - School of Medicine, College of Medicine, I-Shou University, Kaohsiung, Taiwan, R.O.C. AD - Department of Otolaryngology, E-Da Hospital, Kaohsiung, Taiwan, R.O.C. AD - School of Chinese Medicine for Post Baccalaureate, College of Medicine, I-Shou University, Kaohsiung, Taiwan, R.O.C. FAU - Wang, Chih-Chun AU - Wang CC AD - School of Medicine, College of Medicine, I-Shou University, Kaohsiung, Taiwan, R.O.C. AD - Department of Otolaryngology, E-Da Hospital, Kaohsiung, Taiwan, R.O.C. AD - School of Chinese Medicine for Post Baccalaureate, College of Medicine, I-Shou University, Kaohsiung, Taiwan, R.O.C. FAU - Lan, Yu-Yan AU - Lan YY AD - School of Medicine, College of Medicine, I-Shou University, Kaohsiung, Taiwan, R.O.C.; yyinmed@isu.edu.tw. LA - eng PT - Journal Article PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 RN - EC 2.7.10.1 (TYRO3 protein, human) RN - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases) SB - IM MH - Humans MH - *Apoptosis/drug effects MH - *Mouth Neoplasms/drug therapy/pathology/metabolism MH - Cell Line, Tumor MH - Signal Transduction/drug effects MH - Cell Survival/drug effects MH - *Carcinoma, Squamous Cell/drug therapy/pathology/metabolism MH - Receptor Protein-Tyrosine Kinases OTO - NOTNLM OT - BAX OT - Fas OT - OEC-M1 OT - UNC9426 OT - apoptosis OT - oral squamous cell carcinoma EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:04 PHST- 2026/08/03 00:00 [received] PHST- 2026/08/30 00:00 [revised] PHST- 2026/09/04 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:04 [entrez] AID - 46/11/6155 [pii] AID - 10.21873/anticanres.18445 [doi] PST - ppublish SO - Anticancer Res. 2026 Nov;46(11):6155-6168. doi: 10.21873/anticanres.18445. PMID- 42946405 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - Predictors of Pathologic Complete Response Following Neoadjuvant Chemotherapy in Invasive Breast Cancer With Microcalcifications. PG - 6275-6285 LID - 10.21873/anticanres.18456 [doi] AB - BACKGROUND/AIM: Microcalcifications associated with breast cancer often persist after neoadjuvant chemotherapy (NAC), complicating surgical decision-making even when pathologic complete response (pCR) is achieved. This study aimed to identify clinical and imaging factors associated with pCR in invasive breast cancer with microcalcifications following NAC. PATIENTS AND METHODS: This retrospective study included 106 female patients with invasive ductal carcinoma and mammographic microcalcifications who underwent NAC followed by surgery at a single institution between January 2021 and January 2023. Clinicopathological characteristics, breast magnetic resonance imaging (MRI) response, and changes in microcalcifications on mammography were analyzed. Univariate and multivariable logistic regression analyses were performed to identify independent predictors of pCR. RESULTS: The overall pCR rate was 45.3% (48/106). pCR rates by molecular subtype were: HER2-enriched 81.5%, HR+/HER2+ 52.8%, triple-negative 25.0%, and Luminal A 11.1% (p<0.001). Radiologic complete response (rCR) on breast MRI was achieved in 46 patients, of whom 37 (80.4%) achieved pCR (p<0.001). Decreased or disappeared microcalcifications after NAC were associated with a higher, though non-significant, pCR rate (60.9% vs. 41.0%; p=0.266). On multivariable analysis, rCR [odds ratio (OR)=14.29; 95% confidence interval (CI)=5.00-50.00; p<0.001), HER2-enriched subtype (OR=25.00; 95%CI=4.55-100.00; p<0.001), and HR+/HER2+ subtype (OR=5.26; 95%CI=1.15-33.33; p=0.043) were independent predictors of pCR. A predictive model incorporating rCR, molecular subtype, and microcalcification response yielded an area under the ROC curve (AUC) of 0.904. CONCLUSION: In patients with invasive breast cancer and microcalcifications undergoing NAC, HER2-enriched molecular subtype and radiologic complete response on breast MRI are strong independent predictors of pCR. These findings may help surgeons consider less extensive resection of calcified areas in selected patients. CI - Copyright (c) 2026 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Park, Sang Chun AU - Park SC AD - Department of Surgery, Chonnam National University Medical School & Chonnam National University Hwasun Hospital, Gwangju, Republic of Korea. FAU - Ho, Jo Sang AU - Ho JS AD - Department of Surgery, Chonnam National University Medical School & Chonnam National University Hwasun Hospital, Gwangju, Republic of Korea. FAU - Won, Ryu Hee AU - Won RH AD - Department of Surgery, Chonnam National University Medical School & Chonnam National University Hwasun Hospital, Gwangju, Republic of Korea. FAU - Kwon, Yong Bin AU - Kwon YB AD - Department of Surgery, Chonnam National University Medical School & Chonnam National University Hospital, Gwangju, Republic of Korea. FAU - Na, Yong Min AU - Na YM AD - Department of Surgery, Chonnam National University Medical School & Chonnam National University Hwasun Hospital, Gwangju, Republic of Korea. FAU - Ryu, Young Jae AU - Ryu YJ AD - Department of Surgery, Chonnam National University Medical School & Chonnam National University Hwasun Hospital, Gwangju, Republic of Korea. FAU - Lee, Hyo Jae AU - Lee HJ AD - Department of Radiology, Chonnam National University Medical School & Chonnam National University Hwasun Hospital, Gwangju, Republic of Korea. FAU - Lim, Hyo Soon AU - Lim HS AD - Department of Radiology, Chonnam National University Medical School & Chonnam National University Hwasun Hospital, Gwangju, Republic of Korea. FAU - Lee, Ji Shin AU - Lee JS AD - Department of Pathology, Chonnam National University Medical School & Chonnam National University Hwasun Hospital, Gwangju, Republic of Korea. FAU - Cho, Jin Seong AU - Cho JS AD - Department of Surgery, Chonnam National University Medical School & Chonnam National University Hospital, Gwangju, Republic of Korea. FAU - Park, Min Ho AU - Park MH AD - Department of Surgery, Chonnam National University Medical School & Chonnam National University Hwasun Hospital, Gwangju, Republic of Korea; mhpark@chonnam.ac.kr. LA - eng PT - Journal Article PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 RN - EC 2.7.10.1 (Erb-b2 Receptor Tyrosine Kinases) SB - IM MH - Humans MH - Female MH - Pathologic Complete Response MH - *Neoadjuvant Therapy/methods MH - *Breast Neoplasms/drug therapy/pathology/diagnostic imaging MH - *Calcinosis/pathology/diagnostic imaging MH - Retrospective Studies MH - Middle Aged MH - Magnetic Resonance Imaging MH - Adult MH - Aged MH - Mammography MH - *Carcinoma, Ductal, Breast/drug therapy/pathology MH - Erb-b2 Receptor Tyrosine Kinases/metabolism OTO - NOTNLM OT - Breast cancer OT - HER2-enriched OT - mammography OT - microcalcifications OT - neoadjuvant chemotherapy OT - pathologic complete response OT - radiologic complete response EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:04 PHST- 2026/05/19 00:00 [received] PHST- 2026/08/16 00:00 [revised] PHST- 2026/09/10 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:04 [entrez] AID - 46/11/6275 [pii] AID - 10.21873/anticanres.18456 [doi] PST - ppublish SO - Anticancer Res. 2026 Nov;46(11):6275-6285. doi: 10.21873/anticanres.18456. PMID- 42946403 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1791-7530 (Electronic) IS - 0250-7005 (Linking) VI - 46 IP - 11 DP - 2026 Nov TI - AI-assisted Treatment Planning - Only a Vision? A Laboratory Proof-of-Concept in Complex Radiotherapy Treatment Planning Using a Large Language Model for Dose Optimization. PG - 6343-6355 LID - 10.21873/anticanres.18462 [doi] AB - BACKGROUND/AIM: The present study investigates whether volumes of interest (VOIs) derived from local dose deficits in VMAT-based craniospinal irradiation (CSI) can be used for dose optimization within an LLM-guided treatment planning process. Artificial intelligence (AI) may transform radiotherapy treatment planning, but current commercial treatment planning systems (TPS) remain primarily rule- and goal-based and lack dialogue-based decision making during ongoing optimization. MATERIALS AND METHODS: This laboratory proof-of-concept investigated an AI-assisted planning workflow using a large language model (LLM) as a decision-making instance (DMI). Volumetric modulated arc therapy (VMAT) CSI was selected as a challenging scenario. Three strategies were evaluated: a baseline without volumes of interest (VOIs), Experiment A with two predefined VOIs, and Experiment B with VOIs introduced dynamically in response to the evolving dose distribution. RESULTS: The DMI iteratively evaluated dose distributions and planning parameters and recommended objective adjustments to the planning target volume (PTV) and auxiliary structures. The estimated Van't Riet conformity index increased from 0.84 in the baseline experiment to 0.98 in Experiment A and 0.99 in Experiment B. In the baseline experiment, the final PTV D98% was 94.8%, while local dose deficits of approximately 80% remained within the junction regions. In Experiment A, the final D98% values were 98.6% for the PTV, 95.2% for VOI1, and 94.4% for VOI2. In Experiment B, three VOIs were sequentially generated during optimization, and final D98% values reached 96.4% for the PTV, 95.8% for VOI1, 95.6% for VOI2, and 95.0% for VOI3. CONCLUSION: Under laboratory conditions, the LLM-based DMI structured and guided an iterative dose-optimization process, supporting the feasibility of LLM-based planning assistance in radiotherapy. CI - Copyright (c) 2026 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved. FAU - Sperling, Claudia AU - Sperling C AD - Department of Radiation Oncology, University of Lubeck, Lubeck, Germany. FAU - Stille, Maik AU - Stille M AD - Fraunhofer Institute Lubeck, Lubeck, Germany. FAU - Kohnert, Annalena AU - Kohnert A AD - Fraunhofer Institute Lubeck, Lubeck, Germany. FAU - Ziemann, Alexander AU - Ziemann A AD - Gymnasium am Muhlenberg, Bad Schwartau, Germany. FAU - Loser, Anastassia AU - Loser A AD - Department of Radiation Oncology, University of Lubeck, Lubeck, Germany. FAU - Rades, Dirk AU - Rades D AD - Department of Radiation Oncology, University of Lubeck, Lubeck, Germany. FAU - Cremers, Florian AU - Cremers F AD - Department of Radiation Oncology, University of Lubeck, Lubeck, Germany. FAU - Ziemann, Christian AU - Ziemann C AD - Department of Radiation Oncology, University of Lubeck, Lubeck, Germany; christian.ziemann@uksh.de. LA - eng PT - Journal Article PL - Greece TA - Anticancer Res JT - Anticancer research JID - 8102988 SB - IM MH - Humans MH - *Radiotherapy Planning, Computer-Assisted/methods MH - Large Language Models MH - Radiotherapy Dosage MH - Radiotherapy, Intensity-Modulated/methods MH - *Artificial Intelligence MH - Craniospinal Irradiation/methods MH - Proof of Concept Study OTO - NOTNLM OT - Artificial intelligence OT - dose optimization OT - large language model OT - radiotherapy EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 21:04 PHST- 2026/07/24 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:04 [entrez] AID - 46/11/6343 [pii] AID - 10.21873/anticanres.18462 [doi] PST - ppublish SO - Anticancer Res. 2026 Nov;46(11):6343-6355. doi: 10.21873/anticanres.18462. PMID- 42946395 OWN - NLM STAT- Publisher LR - 20261027 IS - 1554-8937 (Electronic) IS - 1554-8929 (Linking) DP - 2026 Oct 28 TI - Small-Molecule Inhibitors of Cholesterol Sulfotransferase as Immunomodulatory Agents. LID - 10.1021/acschembio.6c00658 [doi] AB - Though immune checkpoint blockade (ICB) therapy has been a promising advance in cancer treatment, response rates to ICB are low owing to other mechanisms that cancers employ to evade the immune system. SULT2B1b is an enzyme known to produce cholesterol sulfate (CS), an immunosuppressive metabolite that inhibits T-cell migration. Cancers expressing high levels of SULT2B1b thus generate a tumor microenvironment that excludes T cells, which leads to increased tumor growth and renders ICB therapy ineffective. Here, we identify a novel class of azasteroid SULT2B1b inhibitors with greater potency and selectivity than previously identified compounds. X-ray co-crystal structures indicate that these inhibitors bind in a conformation different from that of native substrates, enabling inhibition without acting as a substrate. Additionally, these new compounds effectively reduce CS levels in tumors in mice and, as such, have potential as cancer therapeutics in combination with ICB therapy. CI - (c) 2026 The Author(s). Published by American Chemical Society. FAU - Boudreau, Lindsay E AU - Boudreau LE AD - Department of Chemistry, University of Illinois Urbana Champaign, Urbana, IL61801, USA. AD - Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana Champaign, Urbana, IL61801, USA. FAU - Smith, Amanda J AU - Smith AJ AUID- ORCID: 0000-0003-3678-1104 AD - Department of Chemistry, University of Illinois Urbana Champaign, Urbana, IL61801, USA. AD - Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana Champaign, Urbana, IL61801, USA. FAU - Lee, Hyang Yeon AU - Lee HY AUID- ORCID: 0000-0003-4361-7871 AD - Department of Chemistry, University of Illinois Urbana Champaign, Urbana, IL61801, USA. AD - Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana Champaign, Urbana, IL61801, USA. FAU - Chakraborty, Bidisha AU - Chakraborty B AD - Center of Biophysics and Quantitative Biology, University of Illinois Urbana Champaign, Urbana, IL61801, USA. FAU - Abo, Kyle R AU - Abo KR AD - Department of Chemistry, University of Illinois Urbana Champaign, Urbana, IL61801, USA. AD - Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana Champaign, Urbana, IL61801, USA. FAU - Chen, Kenneth AU - Chen K AUID- ORCID: 0009-0001-3132-5655 AD - Department of Chemistry, University of Illinois Urbana Champaign, Urbana, IL61801, USA. FAU - Nair, Satish K AU - Nair SK AUID- ORCID: 0000-0003-1790-1334 AD - Center of Biophysics and Quantitative Biology, University of Illinois Urbana Champaign, Urbana, IL61801, USA. AD - Department of Biochemistry, University of Illinois Urbana Champaign, Urbana, IL61801, USA. AD - Cancer Center at Illinois, University of Illinois Urbana Champaign, Urbana, IL61801, USA. FAU - Hergenrother, Paul J AU - Hergenrother PJ AUID- ORCID: 0000-0001-9018-3581 AD - Department of Chemistry, University of Illinois Urbana Champaign, Urbana, IL61801, USA. AD - Carl R. Woese Institute for Genomic Biology, University of Illinois Urbana Champaign, Urbana, IL61801, USA. AD - Department of Biochemistry, University of Illinois Urbana Champaign, Urbana, IL61801, USA. AD - Cancer Center at Illinois, University of Illinois Urbana Champaign, Urbana, IL61801, USA. LA - eng GR - P30CA275774/CA/NCI NIH HHS/United States GR - R35CA283859/CA/NCI NIH HHS/United States GR - R35GM151874/GM/NIGMS NIH HHS/United States PT - Journal Article DEP - 20261028 PL - United States TA - ACS Chem Biol JT - ACS chemical biology JID - 101282906 SB - IM EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 21:03 PHST- 2026/07/17 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:03 [entrez] AID - 5459522 [pii] AID - 10.1021/acschembio.6c00658 [doi] PST - aheadofprint SO - ACS Chem Biol. 2026 Oct 28. doi: 10.1021/acschembio.6c00658. PMID- 42946392 OWN - NLM STAT- Publisher LR - 20261027 IS - 1520-6904 (Electronic) IS - 0022-3263 (Linking) DP - 2026 Oct 28 TI - A Unified Phosgene-Free Carbonylation Using CO2 for the Synthesis of Ureas, Carbamates, Carbonates, Amides, and Formamides. LID - 10.1021/acs.joc.6c01831 [doi] AB - Phosgene remains a privileged reagent for carbonylation; however, its extreme toxicity and stringent regulatory controls often restrict its routine and broad application. Herein, we describe a unified CO2-based carbonylation platform for the synthesis of ureas, (thio)carbamates, (thio)carbonates, amides, and formamides under mild, metal-free conditions. The method employs readily available phosphorus(V) activators to generate transient phosphorus-activated carbonyl species that selectively react with amines, alcohols, thiols, hydrides, and organometallic reagents, enabling a one-pot, three-component protocol that telescopes multi-step phosgene-based sequences into a single operation. This approach eliminates the need for phosgene and other preactivated carbonylating reagents. Mechanistic studies are most consistent with the involvement of a transient phosphorus-mediated carbonylating species, conceptually analogous to electrophilic carbonyl transfer in biological systems, although its precise identity remains unresolved. The synthetic utility of the platform is demonstrated by the 25 g synthesis of the anticancer drug sorafenib using POCl3 as the activator. By harnessing CO2 as a C1 synthon, this strategy addresses a long-standing challenge in electrophilic carbonylation and provides an operationally simple and complementary alternative to phosgene-based methods. CI - (c) 2026 American Chemical Society. FAU - Malkania, Lalit AU - Malkania L AD - Center for Drug Discovery, BRIC-Translational Health Science and Technology Institute, Faridabad, Haryana121001, India. FAU - Kumar, Arun AU - Kumar A AD - Center for Drug Discovery, BRIC-Translational Health Science and Technology Institute, Faridabad, Haryana121001, India. FAU - Kumar, Varun AU - Kumar V AD - Center for Drug Discovery, BRIC-Translational Health Science and Technology Institute, Faridabad, Haryana121001, India. FAU - Rana, Anil AU - Rana A AD - Center for Drug Discovery, BRIC-Translational Health Science and Technology Institute, Faridabad, Haryana121001, India. FAU - Sharma, Pankaj AU - Sharma P AD - Center for Drug Discovery, BRIC-Translational Health Science and Technology Institute, Faridabad, Haryana121001, India. FAU - Sharma, Nutan AU - Sharma N AD - Center for Drug Discovery, BRIC-Translational Health Science and Technology Institute, Faridabad, Haryana121001, India. AD - Department of Chemistry, Faculty of Applied and Basic Sciences, SGT University, Gurugram122505, India. FAU - Sharma, Nidhi AU - Sharma N AD - Center for Drug Discovery, BRIC-Translational Health Science and Technology Institute, Faridabad, Haryana121001, India. FAU - Yadav, Naveen AU - Yadav N AD - Center for Drug Discovery, BRIC-Translational Health Science and Technology Institute, Faridabad, Haryana121001, India. FAU - Mahajan, Dinesh AU - Mahajan D AUID- ORCID: 0000-0002-7939-9506 AD - Center for Drug Discovery, BRIC-Translational Health Science and Technology Institute, Faridabad, Haryana121001, India. LA - eng GR - IIRP-2023-1068/Indian Council of Medical Research/ GR - BT/AIR01609/PACE-28/22/Biotechnology Industry Research Assistance Council/ PT - Journal Article DEP - 20261028 PL - United States TA - J Org Chem JT - The Journal of organic chemistry JID - 2985193R SB - IM EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 21:02 PHST- 2026/07/30 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 21:02 [entrez] AID - 5459520 [pii] AID - 10.1021/acs.joc.6c01831 [doi] PST - aheadofprint SO - J Org Chem. 2026 Oct 28. doi: 10.1021/acs.joc.6c01831. PMID- 42946329 OWN - NLM STAT- Publisher LR - 20261029 IS - 1437-7780 (Electronic) IS - 1341-321X (Linking) VI - 32 IP - 12 DP - 2026 Oct 27 TI - Listeria monocytogenes meningitis after pembrolizumab-based chemoradiotherapy for cervical cancer: A case report. PG - 103114 LID - S1341-321X(26)00212-6 [pii] LID - 10.1016/j.jiac.2026.103114 [doi] AB - Listeria monocytogenes is a major cause of invasive infections in individuals with impaired cell-mediated immunity. Recently, the combination of pembrolizumab with concurrent chemoradiotherapy has been established as the standard treatment for high-risk, locally advanced cervical cancer. Nevertheless, characterization of severe infections that occur during this treatment regimen remains inadequate. Herein, we report a case of L. monocytogenes meningitis that occurred during pembrolizumab-based chemoradiotherapy for cervical cancer. A 45-year-old woman with no underlying medical conditions was diagnosed with p16-positive cervical squamous cell carcinoma, involving the pelvic, para-aortic and left supraclavicular lymph nodes. The patient received concurrent chemoradiotherapy with weekly cisplatin and pembrolizumab every 3 weeks. On day 44, after receiving 50.4 Gy of external beam radiotherapy, she developed febrile neutropenia with a temperature of 40.1  degrees C, white blood cell count of 600/muL, absolute neutrophil count of 408/muL, and worsening diarrhea. She was treated with cefepime, and blood and urine cultures were negative. Immune-related adverse events, including endocrine disorders, were considered unlikely. After clinical improvement, she completed the concurrent chemoradiotherapy phase. On day 73, the patient presented with headache, nausea, and vomiting. Cerebrospinal fluid examination revealed neutrophilic pleocytosis, elevated protein levels, and hypoglycorrhachia. Blood and cerebrospinal fluid cultures yielded L. monocytogenes. She was treated with ampicillin and gentamicin for 3 weeks and recovered. Thus, fever during pembrolizumab-based chemoradiotherapy should not be solely attributed to immune-related adverse events. Severe infections, including Listeria meningitis, should be considered when fever or neurological symptoms occur, particularly in patients with myelosuppression, immune dysregulation, or gastrointestinal toxicity. CI - Copyright (c) 2026 Japanese Society of Chemotherapy, Japanese Association for Infectious Diseases, and Japanese Society for Infection Prevention and Control. Published by Elsevier Ltd. All rights reserved. FAU - Makita, Chiyoko AU - Makita C AD - Department of Radiation Oncology, Gifu Prefectural General Medical Center, 4-6-1, Noisshiki, Gifu-city, 500-8717, Japan. Electronic address: chioko-makita@live.jp. FAU - Yin, Limei AU - Yin L AD - Department of Obstetrics and Gynecology, Gifu Prefectural General Medical Center, 4-6-1, Noisshiki, Gifu-city, 500-8717, Japan. Electronic address: inrimei@yahoo.co.jp. FAU - Kobayashi, Shinichiro AU - Kobayashi S AD - Department of Infection Disease, Gifu Prefectural General Medical Center, 4-6-1, Noisshiki, Gifu-city, 500-8717, Japan. Electronic address: kobayashi-shinichiro@gifu-hp.jp. FAU - Kajiura, Yuichi AU - Kajiura Y AD - Department of Radiation Oncology, Gifu Prefectural General Medical Center, 4-6-1, Noisshiki, Gifu-city, 500-8717, Japan. Electronic address: kajiura-yuichi@gifu-hp.jp. FAU - Tanaka, Osamu AU - Tanaka O AD - Department of Radiation Oncology, Asahi University Hospital, 3-23, Hashimoto-cho, Gifu-city, 500-8523, Japan. Electronic address: c.bluered@gmail.com. FAU - Matsuo, Masayuki AU - Matsuo M AD - Department of Radiation Oncology, Gifu University Hospital, 1-1, Yanagido, Gifu-city, 501-1194, Japan. Electronic address: matsuo.masayuki.e0@f.gifu-u.ac.jp. LA - eng PT - Case Reports PT - Journal Article DEP - 20261027 PL - Netherlands TA - J Infect Chemother JT - Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy JID - 9608375 SB - IM OTO - NOTNLM OT - Cervical cancer OT - Concurrent chemoradiotherapy OT - Listeria monocytogenes OT - Meningitis OT - Pembrolizumab COIS- Conflict of interest None. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:40 PHST- 2026/08/04 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 20:40 [entrez] AID - S1341-321X(26)00212-6 [pii] AID - 10.1016/j.jiac.2026.103114 [doi] PST - aheadofprint SO - J Infect Chemother. 2026 Oct 27;32(12):103114. doi: 10.1016/j.jiac.2026.103114. PMID- 42946316 OWN - NLM STAT- Publisher LR - 20261027 IS - 1556-3871 (Electronic) IS - 1547-5271 (Linking) DP - 2026 Oct 27 TI - Acute effects of cardioneuroablation on ventricular arrhythmia inducibility and refractoriness in patients with functional bradyarrhythmias. The Roman 4 study. LID - S1547-5271(26)02925-5 [pii] LID - 10.1016/j.hrthm.2026.10.053 [doi] AB - BACKGROUND: Cardioneuroablation (CNA) is a promising method to treat functional bradyarrhythmias. However, whether CNA-induced decrease in the parasympathetic drive to the heart may occur proarrhythmic in the future is not known. OBJECTIVE: To assess acute effects of CNA-induced total vagal denervation on QT interval as well as invasively measured ventricular effective refractory period (VERP), ventricular action potential duration (VAPD) and vulnerability to ventricular arrhythmias. METHODS: CNA was performed using anatomical approach by means of electro-anatomical mapping and intracardiac echocardiography. Intra-procedural efficacy was assessed using extracardiac vagal stimulation. QTc and QTd intervals were measured from surface ECG. VAPD and VERP were measured from ablation electrode in right ventricle. Measurements were performed at four time-points: at baseline, immediately after CNA, after atropine injection, and finally after isoproterenol bolus. RESULTS: 80 patients (51 females, mean age 42 +/- 14 years) underwent CNA. Polymorphic ventricular tachyarrhythmias were induced in 2 females who had pre-existing cardiac conditions. Changes in QTc depended on the method used for correction. Using the Bazett formula, QTc was significantly prolonged, however using the Framingham, Fridericia and Hodges corrections, these changes were much less pronounced or non-significant. QTd remained unchanged across all measurements. The VERP and VAPD values remained unchanged after CNA and after atropine whereas they became significantly shorter following isoproterenol injection. CONCLUSION: The present study did not demonstrate evidence of acute electrophysiological changes suggesting increased ventricular arrhythmogenicity after CNA in patients without structural heart disease. However, long-term follow-up is needed to confirm lack of proarrhythmic effects of CNA. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Sikorska, Agnieszka AU - Sikorska A AD - Centre of Postgraduate Medical Education, Grochowski Hospital, Department of Cardiology, Warsaw, Poland. Electronic address: sikorska.agnieszka.anna@gmail.com. FAU - Nowinski, Artur AU - Nowinski A AD - Centre of Postgraduate Medical Education, Grochowski Hospital, Department of Cardiology, Warsaw, Poland. FAU - Niedzwiedz, Michal AU - Niedzwiedz M AD - Centre of Postgraduate Medical Education, Grochowski Hospital, Department of Cardiology, Warsaw, Poland. FAU - Krynski, Tomasz AU - Krynski T AD - Centre of Postgraduate Medical Education, Grochowski Hospital, Department of Cardiology, Warsaw, Poland. FAU - Gawalkiewicz-Mazanska, Anna AU - Gawalkiewicz-Mazanska A AD - Centre of Postgraduate Medical Education, Grochowski Hospital, Department of Cardiology, Warsaw, Poland. FAU - Baran, Jakub AU - Baran J AD - Centre of Postgraduate Medical Education, Grochowski Hospital, Department of Cardiology, Warsaw, Poland. FAU - Kulakowski, Piotr AU - Kulakowski P AD - Centre of Postgraduate Medical Education, Grochowski Hospital, Department of Cardiology, Warsaw, Poland. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Heart Rhythm JT - Heart rhythm JID - 101200317 SB - IM OTO - NOTNLM OT - cardioneuroablation OT - parasympathetic cardiac innervation OT - programmed ventricular stimulation OT - vagal stimulation OT - vasovagal syncope OT - ventricular arrhythmia EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:39 PHST- 2026/06/23 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:39 [entrez] AID - S1547-5271(26)02925-5 [pii] AID - 10.1016/j.hrthm.2026.10.053 [doi] PST - aheadofprint SO - Heart Rhythm. 2026 Oct 27:S1547-5271(26)02925-5. doi: 10.1016/j.hrthm.2026.10.053. PMID- 42946314 OWN - NLM STAT- Publisher LR - 20261027 IS - 1879-1131 (Electronic) IS - 0738-081X (Linking) DP - 2026 Oct 27 TI - Clinical outcomes and inter-rater agreement after 1470-nm interstitial laser facial rejuvenation. LID - S0738-081X(26)00305-6 [pii] LID - 10.1016/j.clindermatol.2026.10.009 [doi] AB - Interstitial 1470-nm laser treatment is used for facial rejuvenation, but published outcomes rely heavily on photographic ratings and inter-rater agreement is seldom reported. This retrospective case series evaluated 3-month aesthetic outcomes, patient satisfaction, recorded procedural tolerability, and agreement between two independent dermatologists in 22 patients. Baseline and 3-month photographs were rated using a 5-point Global Aesthetic Improvement Scale (GAIS; 1, worsened; 5, very much improved). Paired assessor differences were examined with an exact sign test, and agreement was summarized with exact agreement and linearly weighted kappa. Mean age was 46.7 +/- 12.9 years, and 20 patients (90.9%) were women. Mean 3-month satisfaction was 4.59 +/- 0.67 on a 5-point scale; scores ranged from 3 to 5. Median GAIS was 4 (interquartile range [IQR], 3.25-5) for assessor 1 and 5 (IQR, 4-5) for assessor 2. Improvement (GAIS >/=3) was recorded in 20 of 22 patients (90.9%) and 22 of 22 (100%), respectively. Assessor 2 scored 11 patients higher and 2 lower than assessor 1 (exact sign test, P = .0225); exact agreement was 40.9%, and weighted kappa was 0.325 (95% bootstrap confidence interval, 0.075-0.542). Intraprocedural burning sensation and pain were recorded in 59.1% and 27.3% of patients, respectively. This small selected series showed improvement on most 3-month photographic ratings and satisfaction scores concentrated at the upper end of the scale, but the estimated magnitude of improvement depended on the assessor. Reporting assessor-specific score distributions and inter-rater agreement may improve interpretation of photographic outcomes. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Zorlu, Zahide Eris AU - Zorlu ZE AD - Private Dermatology Practice, Antalya, Turkiye. FAU - Kroumpouzos, George AU - Kroumpouzos G AD - Department of Dermatology, Alpert Medical School at Brown University, Providence, RI, USA; APDerm, South Weymouth, MA 02190, USA. Electronic address: gk@gkderm.com. FAU - Tursen, Umit AU - Tursen U AD - Department of Dermatology, School of Medicine, Mersin University, Mersin, Turkiye. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Clin Dermatol JT - Clinics in dermatology JID - 8406412 SB - IM OTO - NOTNLM OT - 1470 nm OT - Endolift OT - Global Aesthetic Improvement Scale OT - facial rejuvenation OT - inter-rater agreement OT - interstitial laser COIS- Declaration of competing interests none declared. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:39 PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:39 [entrez] AID - S0738-081X(26)00305-6 [pii] AID - 10.1016/j.clindermatol.2026.10.009 [doi] PST - aheadofprint SO - Clin Dermatol. 2026 Oct 27:S0738-081X(26)00305-6. doi: 10.1016/j.clindermatol.2026.10.009. PMID- 42946312 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-0984 (Electronic) IS - 0166-0934 (Linking) VI - 348 DP - 2026 Oct 27 TI - Empirical substitution models of evolution for protein phylogenetics of the Crimean-Congo hemorrhagic fever virus. PG - 115494 LID - S0166-0934(26)00160-6 [pii] LID - 10.1016/j.jviromet.2026.115494 [doi] AB - Probabilistic methods for phylogenetic inference rely on substitution models of molecular evolution. However, at the protein level, the availability of empirical substitution models remains limited, particularly for viruses. Here, we present empirical substitution models of protein evolution for several Crimean-Congo hemorrhagic fever virus (CCHFV) proteins, including the nucleoprotein, glycoprotein precursor, and RNA-dependent RNA polymerase. For each protein, we estimated the exchangeability matrix and amino acid frequencies at equilibrium from hundreds of sequences using maximum likelihood, and we evaluated the models using independent data. The inferred CCHFV models revealed distinct evolutionary patterns among viral proteins, including patterns in polar and glycosylation-associated regions, while generally preserving common physicochemical features such as amino acid charge, polarity, and hydrophobic packing shaped by selective pressures. Remarkably, the CCHFV protein substitution models consistently outperformed all currently available empirical substitution models, including those inferred from other viral proteins, in terms of phylogenetic likelihood with independent CCHFV protein sequences. These findings support the use of these substitution models to improve the accuracy of phylogenetic reconstructions of CCHFV proteins, thereby enabling more reliable inference of evolutionary trajectories that may be useful for therapeutic design. CI - Copyright (c) 2026 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Iglesias-Rivas, Paula AU - Iglesias-Rivas P AD - CINBIO, Universidade de Vigo, Vigo, 36310, Spain; Department of Biochemistry, Genetics and Immunology, Universidade de Vigo, Vigo, 36310, Spain. Electronic address: paula.iglesias.rivas@uvigo.es. FAU - Arenas, Miguel AU - Arenas M AD - CINBIO, Universidade de Vigo, Vigo, 36310, Spain; Department of Biochemistry, Genetics and Immunology, Universidade de Vigo, Vigo, 36310, Spain. Electronic address: marenas@uvigo.es. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - J Virol Methods JT - Journal of virological methods JID - 8005839 SB - IM OTO - NOTNLM OT - Crimean-Congo hemorrhagic fever virus OT - Model selection OT - Phylogenetics OT - Protein evolution OT - Substitution model COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:38 PHST- 2026/07/28 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 20:38 [entrez] AID - S0166-0934(26)00160-6 [pii] AID - 10.1016/j.jviromet.2026.115494 [doi] PST - aheadofprint SO - J Virol Methods. 2026 Oct 27;348:115494. doi: 10.1016/j.jviromet.2026.115494. PMID- 42946311 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-4863 (Electronic) IS - 0168-1656 (Linking) DP - 2026 Oct 27 TI - Multilevel metabolic engineering of Bacillus amyloliquefaciens for enhanced L-tyrosine production. LID - S0168-1656(26)00312-3 [pii] LID - 10.1016/j.jbiotec.2026.10.017 [doi] AB - L-Tyrosine is a valuable aromatic amino acid with diverse applications. Although plasmid-based overexpression has markedly improved L-tyrosine production, the dependence on antibiotic selection and genetic instability under nonselective conditions limits its industrial applicability. To address these issues, this study employed a multi-level metabolic engineering strategy to enhance the L-tyrosine biosynthetic pathway at the genomic level in Bacillus amyloliquefaciens. First, an expression cassette containing the mutant prephenate dehydrogenase (TyrA(I309A/E330V)) was inserted into the genome of the wild-type strain Z1. When the copy number reached three (strain Z4), L-tyrosine production reached 437.43mg/L. Subsequently, the mutant DAHP synthase (AroA(R27A/K38A)) was integrated, and the resulting two-copy strain Z7 increased L-tyrosine production to 591.29mg/L. Systematic identification of rate-limiting steps showed that most pathway genes enhanced production to varying extents. A promoter in situ replacement strategy was then applied to construct strain Z21, which further increased the titer to 813.01mg/L. Finally, by optimizing precursor supply, the engineered strain Z32 reached an L-tyrosine titer of 1079.94mg/L. Overall, this plasmid-free and antibiotic-independent platform offers a promising strategy for stable biosynthesis of L-tyrosine and other aromatic amino acids in microbial cell factories. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Zhang, Dong AU - Zhang D AD - School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China; Department of Urology, Jiangnan University Medical Center, Wuxi No. 2 People's Hospital, Wuxi, 214002, China. FAU - Jiang, Pengfei AU - Jiang P AD - Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China; Department of Urology, Jiangnan University Medical Center, Wuxi No. 2 People's Hospital, Wuxi, 214002, China. FAU - Wang, Lanyu AU - Wang L AD - Department of Urology, Jiangnan University Medical Center, Wuxi No. 2 People's Hospital, Wuxi, 214002, China; Department of Urology, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330006, China. FAU - Gu, Jiayu AU - Gu J AD - School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China; Department of Urology, Jiangnan University Medical Center, Wuxi No. 2 People's Hospital, Wuxi, 214002, China. FAU - Ji, Anying AU - Ji A AD - Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100193, China. Electronic address: metaboliceng@163.com. FAU - Feng, Ninghan AU - Feng N AD - Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China; Department of Urology, Jiangnan University Medical Center, Wuxi No. 2 People's Hospital, Wuxi, 214002, China. Electronic address: n.feng@jiangnan.edu.cn. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - J Biotechnol JT - Journal of biotechnology JID - 8411927 SB - IM OTO - NOTNLM OT - Bacillus amyloliquefaciens OT - DAHP synthase OT - L-Tyrosine OT - Metabolic engineering OT - Prephenate dehydrogenase OT - Promoter engineering COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:38 PHST- 2026/04/13 00:00 [received] PHST- 2026/08/31 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:38 [entrez] AID - S0168-1656(26)00312-3 [pii] AID - 10.1016/j.jbiotec.2026.10.017 [doi] PST - aheadofprint SO - J Biotechnol. 2026 Oct 27:S0168-1656(26)00312-3. doi: 10.1016/j.jbiotec.2026.10.017. PMID- 42946304 OWN - NLM STAT- Publisher LR - 20261027 IS - 1615-5947 (Electronic) IS - 0890-5096 (Linking) DP - 2026 Oct 27 TI - Outcomes of single-branched TEVAR with cervical debranching for zone 0 and zone 1 aortic arch repair: A VQI analysis. LID - S0890-5096(26)00742-9 [pii] LID - 10.1016/j.avsg.2026.10.015 [doi] AB - OBJECTIVE: Single-branched thoracic endovascular aortic repair (TEVAR) combined with cervical debranching has emerged as a less invasive alternative to open aortic arch reconstruction for patients requiring zone 0 or zone 1 proximal seal. Contemporary multicenter outcomes of this hybrid approach remain poorly defined. We evaluated procedural characteristics and perioperative outcomes of zone 0 and zone 1 single-branched arch TEVAR with cervical debranching using the Society for Vascular Surgery Vascular Quality Initiative (VQI). METHODS: The VQI TEVAR registry was queried to identify patients undergoing single-branched arch TEVAR with cervical debranching. Patients were included if the proximal landing zone was 0 or 1 and distal disease was confined to zone 5 or more proximal. Chimney, fenestration, stented fenestration, custom-manufactured, and physician-modified repairs were excluded. The zone 0 cohort included patients undergoing innominate side-arm branch repair with left common carotid artery (LCCA) and left subclavian artery (LSA) bypass/transposition, whereas the zone 1 cohort included patients undergoing LCCA side-arm branch repair with LSA bypass/transposition. Outcomes were analyzed descriptively. RESULTS: Among 38,215 TEVAR procedures screened, 50 patients met study criteria, including 39 zone 0 and 11 zone 1 repairs. Mean age was 73.8+/-8.5 years for zone 0 and 71.7+/-12.3 years for zone 1 patients. Prior aortic surgery was common (61.5% vs. 45.5%). Aneurysm was the most frequent indication for repair (66.7% vs. 72.7%). Staged treatment was performed in 74.4% of zone 0 and 45.5% of zone 1 procedures. Median procedure time was 166 and 163 minutes, respectively. Technical success was achieved in all patients. with no conversions to open repair and no completion Type Ia or Ib endoleaks. Cerebrovascular events occurred in 2.6% of zone 0 and 18.2% of zone 1 patients, including stroke in 2.6% and 9.1%, respectively. Spinal cord ischemia occurred in one zone 0 patient (2.6%). Myocardial infarction and postoperative dialysis occurred in 10.3% and 5.1% of zone 0 patients, respectively, and in no zone 1 patients. Thirty-day mortality was 10.3% following zone 0 repair and 0% following zone 1 repair. CONCLUSIONS: Single-branched arch TEVAR with cervical debranching was feasible in selected VQI patients undergoing zone 0 and zone 1 arch repair. Given the small sample size and limited event counts, these findings should be interpreted as descriptive early outcomes rather than evidence of comparative safety or superiority. Cerebrovascular complications remain an important concern after arch endovascular intervention. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Baghbani, Aidin AU - Baghbani A AD - Department of Cardiothoracic and Vascular Surgery, McGovern Medical School at UTHealth Houston, Houston, TX, USA. FAU - Saqib, Naveed U AU - Saqib NU AD - Department of Cardiothoracic and Vascular Surgery, McGovern Medical School at UTHealth Houston, Houston, TX, USA. FAU - Tanaka, Akiko AU - Tanaka A AD - Department of Cardiothoracic and Vascular Surgery, McGovern Medical School at UTHealth Houston, Houston, TX, USA. FAU - Martin, Gordon H AU - Martin GH AD - Department of Cardiothoracic and Vascular Surgery, McGovern Medical School at UTHealth Houston, Houston, TX, USA. FAU - Estrera, Anthony L AU - Estrera AL AD - Department of Cardiothoracic and Vascular Surgery, McGovern Medical School at UTHealth Houston, Houston, TX, USA. FAU - Wang, S Keisin AU - Wang SK AD - Department of Cardiothoracic and Vascular Surgery, McGovern Medical School at UTHealth Houston, Houston, TX, USA. Electronic address: Shihuan.K.Wang@uth.tmc.edu. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Ann Vasc Surg JT - Annals of vascular surgery JID - 8703941 SB - IM OTO - NOTNLM OT - Aortic arch OT - Cervical debranching OT - Hybrid arch repair OT - Thoracic endovascular aortic repair OT - Vascular Quality Initiative OT - Zone 0 OT - Zone 1 EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:38 PHST- 2026/07/06 00:00 [received] PHST- 2026/08/25 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:38 [entrez] AID - S0890-5096(26)00742-9 [pii] AID - 10.1016/j.avsg.2026.10.015 [doi] PST - aheadofprint SO - Ann Vasc Surg. 2026 Oct 27:S0890-5096(26)00742-9. doi: 10.1016/j.avsg.2026.10.015. PMID- 42946300 OWN - NLM STAT- Publisher LR - 20261027 IS - 1097-6833 (Electronic) IS - 0022-3476 (Linking) DP - 2026 Oct 27 TI - Early Detection of Cerebral Palsy in Ireland: A National NICU Implementation Initiative. PG - 115377 LID - S0022-3476(26)00405-1 [pii] LID - 10.1016/j.jpeds.2026.115377 [doi] AB - OBJECTIVE: To implement standardized early cerebral palsy (CP) detection pathways nationally across all tertiary neonatal intensive care unit (NICU) follow-up programs in Ireland, with the aim to reduce the median age of CP diagnosis to 30 years). Additionally, machine learning algorithms were applied to identify robust biomarker candidates. By intersecting multiple linear regression and machine learning outputs, we consistently identified miR-1255b-5p (AUC 0.861-0.768) and miR-433-5p (AUC 0.744-0.611) as potential biomarkers of overall exposure. Additionally, miR-620 (AUC 0.938-0.785) was associated with shorter exposure periods, while miR-1269a (AUC 1.000-0.820) and miR-519d-3p (AUC 0.812-0.719) were selected for long-term exposure. Significant correlations were found between specific miRNAs and quantified pesticide residues, notably a negative correlation between miR-1269a and imidacloprid metabolite (r = -0.53, p = 0.03) and positive correlations with carbofuran (r = 0.57, p = 0.02) and desdimethyldiuron (r = 0.57, p = 0.02). Our findings provide new evidence that occupational pesticide exposure is associated with specific alterations in circulating miRNA profiles. miRNA profiling has emerged as a promising tool for risk assessment, though longitudinal studies with larger cohorts are warranted to validate these biomarkers. CI - Copyright (c) 2026 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Lima, Isabela Barros AU - Lima IB AD - Molecular Oncology Research Center, Barretos Cancer Hospital, SP, Brazil. FAU - Francisco, Luiza Flavia Veiga AU - Francisco LFV AD - Molecular Oncology Research Center, Barretos Cancer Hospital, SP, Brazil. FAU - Birolli, Willian Garcia AU - Birolli WG AD - Chromatography Group, Sao Carlos Institute of Chemistry, University of Sao Paulo, SP, Brazil. FAU - Dos Santos Neto, Alvaro Jose AU - Dos Santos Neto AJ AD - University Center of the Barretos Educational Foundation (UNIFEB), Barretos, Sao Paulo, Brazil. FAU - Marques, Marcia Maria Chiquitelli AU - Marques MMC AD - Molecular Oncology Research Center, Barretos Cancer Hospital, SP, Brazil; University Center of the Barretos Educational Foundation (UNIFEB), Barretos, Sao Paulo, Brazil. FAU - Silveira, Henrique C S AU - Silveira HCS AD - Molecular Oncology Research Center, Barretos Cancer Hospital, SP, Brazil; University Center of the Barretos Educational Foundation (UNIFEB), Barretos, Sao Paulo, Brazil. Electronic address: henriquecssilveira@gmail.com. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Environ Pollut JT - Environmental pollution (Barking, Essex : 1987) JID - 8804476 SB - IM OTO - NOTNLM OT - Biomarkers OT - Epigenetics OT - Machine learning OT - Occupational exposure OT - Pesticides OT - microRNAs COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Isabela Barros Lima reports financial support was provided by Sao Paulo Research Foundation (FAPESP). Isabela Barros Lima reports a relationship with Sao Paulo Research Foundation (FAPESP) that includes: funding grants. Isabela Barros Lima reports a relationship with Coordination for the Improvement of Higher Education Personnel (CAPES) that includes: funding grants. Henrique C. S. Silveira reports a relationship with National Council for Scientific and Technological Development (CNPq) that includes: funding grants. Marcia M. C. Marques reports a relationship with National Council for Scientific and Technological Development (CNPq) that includes: funding grants. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:38 PHST- 2026/06/03 00:00 [received] PHST- 2026/08/04 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 20:38 [entrez] AID - S0269-7491(26)01775-6 [pii] AID - 10.1016/j.envpol.2026.129405 [doi] PST - aheadofprint SO - Environ Pollut. 2026 Oct 27;411(Pt B):129405. doi: 10.1016/j.envpol.2026.129405. PMID- 42946292 OWN - NLM STAT- Publisher LR - 20261027 IS - 1096-0309 (Electronic) IS - 0003-2697 (Linking) DP - 2026 Oct 27 TI - Eco-Friendly Nanoparticle-Modified Electrochemical Sensor for Determination of Alogliptin: Novel Application to Pharmacokinetics, Plasma Protein Binding, and Greenness Assessment. PG - 116286 LID - S0003-2697(26)00242-3 [pii] LID - 10.1016/j.ab.2026.116286 [doi] AB - Alogliptin (ALO), as a dipeptidyl peptidase-4 (DPP-4) inhibitor is one of the commonly prescribed oral hypoglycemics for its potential effects on incretin and glucagon levels. For the first time silver nanoparticles (AgNPs) were introduced into the polymeric membrane cocktail of a newly developed ALO-selective electrode as ion-to-electron transducers. The optimized sensor achieved a near-Nernstian slope of 60.43 and 58.66 mV/decade as authentic and spiked plasma samples, respectively over a linear range of 8.60x10(-7)-1.55x10(-3) M with a limit of detection of 2.86x10(-7) M. The membrane blend involves also; tricresyl phosphate (TCP) as plasticizer and phosphotungestic acid (PT) as cationic exchanger with almost typical Nernstian slope. Testing water layer formation has proven no negative effects on the electrode performance with acceptable operational lifetime and fast dynamic response time. Validation of the experimental parameters has experienced mean percentage recoveries of 95.00 % and relative standard deviation values (%RSD) less than 5.00 %. An average recovery percentage of approximately 99.00 % was obtained upon application to commercial tablets without significant differences with a previously reported method. The proposed sensor has been exploited as unique application in determining ALO - rabbit plasma binding interaction kinetics through constructing Hill plot, binding isotherms and Vant' Hoff plot in different temperatures. The calculated parameters have proven both spontaneity and positive cooperative binding. Additionally, this sensor has been used as a proof-of-concept demonstration to track the pharmacokinetic behavior of ALO in rabbit males, illustrating its in-vivo pathway. The method was assessed using AGREE, AGREEprep, Complex-GAPI, and BAGI greenness/blueness metrics (scores of 0.69, 0.74, 79, and 77.5, respectively), which are reported and discussed below rather than characterized only qualitatively. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Hakiem, Ahmed Faried Abdel AU - Hakiem AFA AD - Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Qena University, Qena, Egypt- 83523. Electronic address: ahmed_faried@pharm.kfs.edu.eg. FAU - Sayed, Sara Mostafa AU - Sayed SM AD - Chemistry Department, Faculty of Science , Assiut University, Assiut, Egypt. FAU - Haredy, Ahmed M AU - Haredy AM AD - Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Merit University, New Sohag, Egypt. FAU - Draz, Mohammed E AU - Draz ME AD - Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa 11152, Egypt. FAU - Mahfouz, Refat M AU - Mahfouz RM AD - Chemistry Department, Faculty of Science , Assiut University, Assiut, Egypt. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Anal Biochem JT - Analytical biochemistry JID - 0370535 SB - IM OTO - NOTNLM OT - AgNPs modified ISE OT - Alogliptin OT - Greenness assessment OT - Novel monitoring of binding interaction to rabbit plasma OT - Validation study and application to tablets OT - in-vivo pharmacokinetic study COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal conflicts that could have appeared to influence the work reported in this manuscript EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:38 PHST- 2026/06/26 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:38 [entrez] AID - S0003-2697(26)00242-3 [pii] AID - 10.1016/j.ab.2026.116286 [doi] PST - aheadofprint SO - Anal Biochem. 2026 Oct 27:116286. doi: 10.1016/j.ab.2026.116286. PMID- 42946288 OWN - NLM STAT- Publisher LR - 20261028 IS - 1879-0003 (Electronic) IS - 0141-8130 (Linking) VI - 385 DP - 2026 Oct 27 TI - Oxidized pullulan-zein-caseinate hybrid nanoparticles for piperine delivery: Rational design supported by molecular dynamics simulations. PG - 155032 LID - S0141-8130(26)04984-6 [pii] LID - 10.1016/j.ijbiomac.2026.155032 [doi] AB - Polysaccharides offer unique molecular scaffolding that can significantly enhance the stability and functionality of protein-based nanocarriers. Here, we demonstrate that oxidized pullulan critically stabilizes zein-caseinate nanoparticles through a combined computational and experimental strategy. Molecular dynamics simulations first revealed that pullulan promotes extensive hydrogen bonding, reduces conformational fluctuations, and compacts the protein assembly, predicting enhanced structural integrity and pH-responsive behavior. These simulations provided complementary molecular-level insights into OP-protein interactions, which, together with experimental considerations, supported the subsequent development of the nanoparticle system. Oxidized pullulan was covalently conjugated to caseinate via Schiff base chemistry and integrated into zein nanoparticles. Experimental characterization confirmed selective oxidation, efficient caseinate-oxidized pullulan conjugation, and preservation of the polymer backbone, resulting in nanoparticles with uniform size ( approximately 220 nm), narrow PDI, high redispersibility, and remarkable colloidal stability under physiological and serum-rich conditions. Piperine, a hydrophobic anticancer agent, was efficiently encapsulated, exhibiting sustained, pH-triggered release (up to approximately 73% at pH 5.5) consistent with the predicted polysaccharide-mediated stabilization. In vitro cytotoxicity demonstrated preferential anti-tumor activity MDA-MB-231 cells (IC(5)(0) = 129 mug/mL) while sparing normal fibroblasts (IC(5)(0) = 176 mug/mL). Confocal microscopy showed higher cell-associated fluorescence in MDA-MB-231 cells than in HFF cells, consistent with differential cellular association. Collectively, these findings demonstrate the potential of pullulan-functionalized protein nanoparticles to provide improved stability, redispersibility, and pH-dependent drug release, together with differential cellular association, supporting their potential as protein-polysaccharide nanocarriers for anticancer drug delivery. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Moazeni, Fatemeh AU - Moazeni F AD - Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran. FAU - Pourhossein, Atefeh AU - Pourhossein A AD - Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran. FAU - Rezaei, Shokouh AU - Rezaei S AD - Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. FAU - Bagheri, Fatemeh AU - Bagheri F AD - Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran. FAU - Shojaosadati, Seyed Abbas AU - Shojaosadati SA AD - Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran. Electronic address: shoja_sa@modares.ac.ir. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Int J Biol Macromol JT - International journal of biological macromolecules JID - 7909578 SB - IM OTO - NOTNLM OT - Breast Cancer therapy OT - Caseinate conjugates OT - Molecular dynamics OT - Pullulan OT - Zein OT - pH-responsive COIS- Declaration of competing interest The authors declare that they have no competing interests. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:38 PHST- 2026/08/15 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 20:38 [entrez] AID - S0141-8130(26)04984-6 [pii] AID - 10.1016/j.ijbiomac.2026.155032 [doi] PST - aheadofprint SO - Int J Biol Macromol. 2026 Oct 27;385:155032. doi: 10.1016/j.ijbiomac.2026.155032. PMID- 42946278 OWN - NLM STAT- Publisher LR - 20261027 IS - 1879-0461 (Electronic) IS - 1040-8428 (Linking) DP - 2026 Oct 27 TI - Molecular and Cellular TCR-Mimic Therapeutic Platforms for Cancer Therapy: A Comparative Review toward a Context-Driven Selection Framework. PG - 105673 LID - S1040-8428(26)00560-3 [pii] LID - 10.1016/j.critrevonc.2026.105673 [doi] AB - T-cell receptor-mimic antibodies (TCRm-Abs) have enabled the specific targeting of peptide-major histocompatibility complexes (p-MHCs), thereby granting access to intracellular tumor antigens that are typically inaccessible to conventional antibody therapeutics. Nevertheless, the extremely low density of these complexes on the surface of tumor cells has remained the primary challenge for all TCRm-Ab-based platforms. This review provides a critical comparison of various molecular platforms-including TCRm-immunoconjugates (TCRm-ICs) and TCRm-bispecific T-cell engagers (TCRm BiTEs)-as well as cellular platforms, such as TCRm-chimeric antigen receptor (TCRm-CAR), antibody T-cell receptor (AbTCR), and Synthetic TCR and Antigen Receptor (STAR). It evaluates their design and optimization strategies to overcome low antigen density, mechanisms of action, translational potential, and current challenges. Immunoconjugates rely on payload potency and linker stability, engager molecules achieve serial killing through T cells, whereas cellular platforms-particularly STAR-attain antigen sensitivity comparable to that of native T-cell receptors by mimicking the TCRalphabeta architecture. Nonetheless, the durable clinical translation of these modalities remains limited. Based on these insights, we propose a context-driven selection framework that integrates molecular engineering, modulation of the tumor microenvironment, combination immunotherapy, and quantitative tumor characteristics to inform the preclinical development and rational selection of TCRm therapeutic platforms. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Mollamohammadi, Fahimeh AU - Mollamohammadi F AD - Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Hezar Jarib Street, Isfahan 81746-73441, Iran. Electronic address: f.mollamohammadi@sci.ui.ac.ir. FAU - Emamzadeh, Rahman AU - Emamzadeh R AD - Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Hezar Jarib Street, Isfahan 81746-73441, Iran. Electronic address: R.Emamzadeh@sci.ui.ac.ir. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Netherlands TA - Crit Rev Oncol Hematol JT - Critical reviews in oncology/hematology JID - 8916049 SB - IM OTO - NOTNLM OT - Cancer immunotherapy OT - Cellular immunotherapy OT - Peptide-major histocompatibility complex (p-MHC) OT - T-cell receptor-mimic antibodies (TCRm-Abs) OT - TCRm therapeutic platforms OT - TCRm- immunoconjugates OT - TCRm-engagers COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Declaration of Competing Interest The authors, Fahimeh Mollamohammadi and Rahman Emamzadeh, declare that they have nothing to declare. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:38 PHST- 2026/08/13 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:38 [entrez] AID - S1040-8428(26)00560-3 [pii] AID - 10.1016/j.critrevonc.2026.105673 [doi] PST - aheadofprint SO - Crit Rev Oncol Hematol. 2026 Oct 27:105673. doi: 10.1016/j.critrevonc.2026.105673. PMID- 42946277 OWN - NLM STAT- Publisher LR - 20261027 IS - 1879-0461 (Electronic) IS - 1040-8428 (Linking) DP - 2026 Oct 27 TI - Bispecific Immune Cell Engagers for Solid Tumors: Molecular Engineering and Therapeutic Perspectives. PG - 105676 LID - S1040-8428(26)00563-9 [pii] LID - 10.1016/j.critrevonc.2026.105676 [doi] AB - Bispecific immune cell engagers are among the most promising modalities in cancer immunotherapy, having made a significant impact on the treatment of hematological malignancy. However, their translation to solid tumors has remained challenging, leaving a critical gap for most cancer patients. A central obstacle is the lack of a mechanistic framework that integrates the complex biology of solid tumors with clinical outcomes, preventing a coherent interpretation of trial failures and impeding the development of more effective strategies. This review addresses that gap. We have reviewed clinical data from diverse engager formats recruiting T cells, NK cells, gammadelta T cells and monocytes across both successful and discontinued programs, showing how target selection, antigen accessibility, effector choice and molecular engineering collectively determine efficacy, half-life, tolerability and durability of response. We thereby construct a clinically grounded framework for designing next-generation engagers. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Kumari, Priya AU - Kumari P AD - Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Bhopal, Madhya Pradesh 462066, India. FAU - Srivastava, Ayush AU - Srivastava A AD - Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Bhopal, Madhya Pradesh 462066, India. FAU - Raul, Sneha Sandip AU - Raul SS AD - Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Bhopal, Madhya Pradesh 462066, India. FAU - Priyanjali, B N AU - Priyanjali BN AD - Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Bhopal, Madhya Pradesh 462066, India. FAU - Dey, Arundhuti AU - Dey A AD - Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Bhopal, Madhya Pradesh 462066, India. FAU - Bordoloi, Devivasha AU - Bordoloi D AD - Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Bhopal, Madhya Pradesh 462066, India. Electronic address: devivasha@iiserb.ac.in. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Netherlands TA - Crit Rev Oncol Hematol JT - Critical reviews in oncology/hematology JID - 8916049 SB - IM OTO - NOTNLM OT - Bispecific Immune cell engager OT - antibody engineering OT - cancer immunotherapy OT - clinical trial outcomes OT - solid tumor OT - translational oncology COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:38 PHST- 2026/08/11 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:38 [entrez] AID - S1040-8428(26)00563-9 [pii] AID - 10.1016/j.critrevonc.2026.105676 [doi] PST - aheadofprint SO - Crit Rev Oncol Hematol. 2026 Oct 27:105676. doi: 10.1016/j.critrevonc.2026.105676. PMID- 42946269 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-3476 (Electronic) IS - 0378-5173 (Linking) DP - 2026 Oct 27 TI - MMP-responsive multiwalled carbon nanotubes enable tumor-selective drug delivery with altered intracellular trafficking and reduced off-target toxicity. PG - 127606 LID - S0378-5173(26)01055-0 [pii] LID - 10.1016/j.ijpharm.2026.127606 [doi] AB - Doxorubicin (DOX) is a widely used chemotherapeutic agent, but its clinical efficacy is limited by multidrug resistance (MDR), poor tumor penetration, and off-target toxicity. To address these challenges, we developed matrix metalloproteinase (MMP)-responsive multiwalled carbon nanotubes (MWCNTs) for tumor-targeted DOX delivery. The MWCNT formulation exhibited MMP-responsive cellular uptake, cell permeability, tissue penetration, and enhanced cytotoxicity of loaded DOX in cancer cells, a transwell model, and tumor spheroids. Mechanistic studies revealed that these CNTs entered cells primarily via clathrin-mediated endocytosis. Acidic endosomal conditions promoted lipid redistribution and membrane destabilization of endolysosomes, facilitating efficient endosomal escape and strong colocalization with the endoplasmic reticulum (ER). This intracellular trafficking pathway bypassed drug efflux mechanisms and prolonged intracellular drug retention in MDR cancer cells. In a mouse breast tumor model, MMP-responsive MWCNTs resulted in approximately 5-fold higher blood DOX levels and 2-fold greater tumor DOX accumulation than free DOX at the end of the treatment regimen. Improved pharmacokinetics and tumor specificity enhanced DOX's tumor inhibitory effects and increased tumor cell apoptosis. Importantly, the MMP-responsive MWCNTs significantly reduced DOX's off-target toxicity, as evidenced by approximately 2-fold lower cardiac DOX accumulation, reduced cardiomyocyte apoptosis, and preservation of white blood cell counts. Overall, this MMP-responsive CNT-based nanoplatform offers a promising strategy to enhance tumor targeting and retention, overcome MDR, and improve the therapeutic index of DOX. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Bhattarai, Prapanna AU - Bhattarai P AD - Department of Pharmaceutical Sciences, Irma Lerma Rangel College of Pharmacy, Texas A&M University, College Station, TX 77843, United States. FAU - Iqbal, Asif AU - Iqbal A AD - Department of Pharmaceutical Sciences, Irma Lerma Rangel College of Pharmacy, Texas A&M University, College Station, TX 77843, United States. FAU - Yadav, Umakant AU - Yadav U AD - Department of Pharmaceutical Sciences, Irma Lerma Rangel College of Pharmacy, Texas A&M University, College Station, TX 77843, United States. FAU - Qadri, Shahnaz Majid AU - Qadri SM AD - Department of Pharmaceutical Sciences, Irma Lerma Rangel College of Pharmacy, Texas A&M University, College Station, TX 77843, United States. FAU - Ara, Nishat AU - Ara N AD - Department of Pharmaceutical Sciences, Irma Lerma Rangel College of Pharmacy, Texas A&M University, College Station, TX 77843, United States. FAU - Mandal, Saptarshi AU - Mandal S AD - Department of Pharmaceutical Sciences, Irma Lerma Rangel College of Pharmacy, Texas A&M University, College Station, TX 77843, United States. FAU - Foote, Aaron AU - Foote A AD - Biopact Ventures, LLC, 12310 Trail Driver Street, Austin, TX 78737, United States. FAU - Castillo, Kevin AU - Castillo K AD - Biopact Ventures, LLC, 12310 Trail Driver Street, Austin, TX 78737, United States. FAU - Suen Cheng, Samuel Kok AU - Suen Cheng SK AD - College of Engineering, Texas A&M University-Corpus Christi, Corpus Christi, TX 78412, United States. FAU - Sheng, Jian AU - Sheng J AD - College of Engineering, Texas A&M University-Corpus Christi, Corpus Christi, TX 78412, United States. FAU - Khasawneh, Fadi AU - Khasawneh F AD - Department of Pharmaceutical Sciences, Irma Lerma Rangel College of Pharmacy, Texas A&M University, College Station, TX 77843, United States. FAU - Zhu, Lin AU - Zhu L AD - Department of Pharmaceutical Sciences, Irma Lerma Rangel College of Pharmacy, Texas A&M University, College Station, TX 77843, United States. Electronic address: lzhu@tamu.edu. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Int J Pharm JT - International journal of pharmaceutics JID - 7804127 SB - IM OTO - NOTNLM OT - Carbon nanotubes (CNTs) OT - Doxorubicin OT - ER targeting OT - MMP-responsive OT - Multi drug resistant (MDR) OT - Off-target toxicity OT - Tumor-targeted drug delivery COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:38 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/22 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:38 [entrez] AID - S0378-5173(26)01055-0 [pii] AID - 10.1016/j.ijpharm.2026.127606 [doi] PST - aheadofprint SO - Int J Pharm. 2026 Oct 27:127606. doi: 10.1016/j.ijpharm.2026.127606. PMID- 42946268 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-3476 (Electronic) IS - 0378-5173 (Linking) DP - 2026 Oct 27 TI - A structure-based virtual screening-driven discovery pharmaceutics platform for cancer therapeutics development: FDA 505(b)(2) repurposing of netupitant as a BMX-targeted nanomedicine using quality-by-design lipid nanoparticles and model-informed PBPK translation. PG - 127608 LID - S0378-5173(26)01057-4 [pii] LID - 10.1016/j.ijpharm.2026.127608 [doi] AB - Bone marrow tyrosine kinase on chromosome X (BMX) is an emerging therapeutic target implicated in prostate cancer progression, metastatic disease, and resistance to current therapies; however, no selective BMX-targeted therapy has advanced to clinical development. Building upon our previously established AI-driven BMX discovery platform, this study presents an integrated Discovery Pharmaceutics strategy combining artificial intelligence-assisted drug repurposing, Quality-by-Design (QbD)-guided lipid nanoparticle (LNP) formulation, experimental validation, and physiologically based pharmacokinetic (PBPK) modeling to accelerate clinical translation through the FDA 505(b)(2) regulatory pathway. An structure-based virtual screening workflow comprising 2,115 FDA-approved small molecules identified netupitant as one of the highest-ranked candidates against the BMX catalytic domain, exhibiting a predicted binding affinity of -10.9 kcal/mol together with favorable selectivity over closely related Tec-family kinases. Netupitant demonstrated concentration-dependent inhibition of prostate cancer cell growth with IC(50) values of 15.97 muM in DU145 cells and 7.1 muM in PC3 cells. A Box-Behnken Design optimized Netupitant-loaded lipid nanoparticles (Netupitant-LNPs) with a particle size of 133.9 +/- 3.2 nm, polydispersity index of 0.213 +/- 0.005, zeta potential of + 30 +/- 1.3 mV, and encapsulation efficiency of 97.3 +/- 0.7%. Cryogenic transmission electron microscopy confirmed uniformly dispersed spherical nanoparticles with an average diameter of 130 +/- 6 nm, consistent with dynamic light scattering measurements. The nanoformulation substantially enhanced therapeutic efficacy, reducing IC(50) values to 1.1 muM in DU145 cells and 0.5 muM in PC3 cells, representing approximately 15-fold and 14-fold improvements, respectively, over free netupitant. To facilitate clinical translation, experimentally determined formulation characteristics were incorporated into a mechanistic Simcyp(R) Version 25.2 middle-out physiologically based pharmacokinetic model. The exploratory PBPK model compared alternative intravenous dosing scenarios and identified 100 mg once weekly as a candidate regimen for further evaluation; these simulations do not establish a clinical dosing recommendation. Collectively, this work demonstrates that integrating AI-enabled drug repurposing, Discovery Pharmaceutics, nanomedicine engineering, and model-informed drug development provides an effective strategy for accelerating oncology drug development. Netupitant-LNPs emerge as a repurposed nanomedicine candidate with predicted BMX binding while establishing a broadly applicable translational framework for FDA-approved drug repurposing through the 505(b)(2) pathway. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Singirikonda, Manasa AU - Singirikonda M AD - Division of Pharmaceutical Sciences, Arnold & Marie Schwartz College of Pharmacy and Health Sciences, Long Island University, 75 DeKalb Avenue, Brooklyn, NY 11201, USA. FAU - Patel, Tarang AU - Patel T AD - Division of Pharmaceutical Sciences, Arnold & Marie Schwartz College of Pharmacy and Health Sciences, Long Island University, 75 DeKalb Avenue, Brooklyn, NY 11201, USA. FAU - Parupathi, Prashanth AU - Parupathi P AD - Division of Pharmaceutical Sciences, Arnold & Marie Schwartz College of Pharmacy and Health Sciences, Long Island University, 75 DeKalb Avenue, Brooklyn, NY 11201, USA. FAU - Nagarajan, Akshaya AU - Nagarajan A AD - Division of Pharmaceutical Sciences, Arnold & Marie Schwartz College of Pharmacy and Health Sciences, Long Island University, 75 DeKalb Avenue, Brooklyn, NY 11201, USA. FAU - Patel, Jainam Shaileshkumar AU - Patel JS AD - Division of Pharmaceutical Sciences, Arnold & Marie Schwartz College of Pharmacy and Health Sciences, Long Island University, 75 DeKalb Avenue, Brooklyn, NY 11201, USA. FAU - Devarakonda, Lakshmisirisha AU - Devarakonda L AD - Division of Pharmaceutical Sciences, Arnold & Marie Schwartz College of Pharmacy and Health Sciences, Long Island University, 75 DeKalb Avenue, Brooklyn, NY 11201, USA. FAU - Kumar, Avinash AU - Kumar A AD - Division of Pharmaceutical Sciences, Arnold & Marie Schwartz College of Pharmacy and Health Sciences, Long Island University, 75 DeKalb Avenue, Brooklyn, NY 11201, USA. FAU - Benzeroual, Kenza E AU - Benzeroual KE AD - Division of Pharmaceutical Sciences, Arnold & Marie Schwartz College of Pharmacy and Health Sciences, Long Island University, 75 DeKalb Avenue, Brooklyn, NY 11201, USA. FAU - Dave, Rutesh H AU - Dave RH AD - Division of Pharmaceutical Sciences, Arnold & Marie Schwartz College of Pharmacy and Health Sciences, Long Island University, 75 DeKalb Avenue, Brooklyn, NY 11201, USA. FAU - Jumaa, Mouhannad AU - Jumaa M AD - Fekra Therapeutics Inc., Boston, MA, USA. FAU - Al-Fayoumi, Suliman AU - Al-Fayoumi S AD - Fekra Therapeutics Inc., Boston, MA, USA. FAU - Nazzal, Sami AU - Nazzal S AD - College of Pharmacy, Texas Tech University Health Sciences Center, Dallas, TX, USA; Office of Research and Standards (ORS), Office of Generic Drugs (OGD), Center for Drug Evaluation and Research (CDER), U.S. Food and Drug Administration (FDA), 10903 New Hampshire Avenue, Silver Spring, MD, USA. FAU - Fayyad, Ahmed Abu AU - Fayyad AA AD - Division of Pharmaceutical Sciences, Arnold & Marie Schwartz College of Pharmacy and Health Sciences, Long Island University, 75 DeKalb Avenue, Brooklyn, NY 11201, USA. Electronic address: Ahmed.AbuFayyad@liu.edu. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Int J Pharm JT - International journal of pharmaceutics JID - 7804127 SB - IM OTO - NOTNLM OT - Artificial intelligence OT - Bone marrow tyrosine kinase on chromosome X (BMX) OT - Cancer therapeutics OT - Discovery pharmaceutics OT - Drug repurposing OT - FDA 505(b)(2) OT - Lipid nanoparticles (LNPs) OT - Model-informed drug development (MIDD) OT - Physiologically based pharmacokinetic (PBPK) modeling COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:38 PHST- 2026/07/26 00:00 [received] PHST- 2026/10/21 00:00 [revised] PHST- 2026/10/24 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:38 [entrez] AID - S0378-5173(26)01057-4 [pii] AID - 10.1016/j.ijpharm.2026.127608 [doi] PST - aheadofprint SO - Int J Pharm. 2026 Oct 27:127608. doi: 10.1016/j.ijpharm.2026.127608. PMID- 42946252 OWN - NLM STAT- Publisher LR - 20261027 IS - 1538-2990 (Electronic) IS - 0002-9629 (Linking) DP - 2026 Oct 27 TI - Metabolic Burden, Metabolic Dysfunction-Associated Steatotic Liver Disease, and Cognitive Performance Among Older Adults: Analysis of 1,387 Participants from the National Health and Nutrition Examination Survey. LID - S0002-9629(26)00408-8 [pii] LID - 10.1016/j.amjms.2026.10.009 [doi] AB - BACKGROUND: Metabolic dysfunction-associated steatotic liver disease (MASLD) is prevalent in older adults, yet its independent contribution to cognitive decline beyond cardiometabolic risk factors remains uncertain. METHODS: We performed a cross-sectional analysis of 1,387 NHANES 2011-2014 participants aged >/=60 years. MASLD was defined using hepatic steatosis index >/=36 plus >/=1 cardiometabolic risk factor, excluding excessive alcohol use. Cognitive performance across memory, verbal fluency, and processing speed was summarized as a global z-score composite. Advanced fibrosis likelihood was assessed using validated non-invasive scores. Weighted multivariable linear regression adjusted for demographic, lifestyle, and cardiometabolic covariates. FINDINGS: MASLD was not associated with global cognition (beta = 0.027; p=0.526) or individual domains after full adjustment. Advanced fibrosis risk was unrelated to cognition except for lower CERAD memory with high FIB-4 (beta = -0.319; p=0.016). Greater metabolic risk factor burden showed graded associations with poorer cognition: >/=2 risk factors linked to lower global cognition (beta = -0.23; p=0.036) and memory (beta = -0.33; p=0.003); >/=3 showed borderline memory impairment (beta = -0.24; p=0.049). In MASLD participants, >/=2 risk factors were associated with lower global cognition (beta = -0.19; p=0.021) and >/=3 with reduced verbal fluency (beta = -0.27; p=0.045). Processing speed declined only at highest metabolic burden in fibrosis-stratified models (e.g., DSST beta = -0.51; p=0.015). CONCLUSION: MASLD and related fibrosis risk were not independently linked to cognitive impairment. Metabolic risk factor burden primarily drove poorer cognition, especially memory, in both the overall sample and those with MASLD, with processing speed affected only at the highest burden. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Aldiabat, Mohammad AU - Aldiabat M AD - Department of Medicine, Washington University in St Louis, St Louis, Missouri, United States of America; Department of Epidemiology, Harvard T. H. Chan School of Public Health, Boston, Massachusetts, United States of America. FAU - Aljabiri, Yazan AU - Aljabiri Y AD - Department of Medicine, Washington University in St Louis, St Louis, Missouri, United States of America; Department of Epidemiology, Harvard T. H. Chan School of Public Health, Boston, Massachusetts, United States of America. FAU - Aleyadeh, Wesam AU - Aleyadeh W AD - National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (NIH), Bethesda, Maryland, United States of America. FAU - Essa, Mohammed AU - Essa M AD - Richard A. and Susan F. Smith Center for Outcomes Research, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA. FAU - Basil Nasir, Ahmad AU - Basil Nasir A AD - Division of Gastroenterology and Hepatology, Saint Louis University, St Louis, Missouri, United States of America. FAU - J Frey, Lewis AU - J Frey L AD - Division of Gastroenterology and Hepatology, Saint Louis University, St Louis, Missouri, United States of America; VA St. Louis Health Care System, St Louis, Missouri, United States of America; Department of Pathology, Saint Louis University, St Louis, Missouri, United States of America. FAU - D Farmer, Adam AU - D Farmer A AD - Division of Gastroenterology and Hepatology, Saint Louis University, St Louis, Missouri, United States of America; Department of Pathology, Saint Louis University, St Louis, Missouri, United States of America. FAU - Syn, Wing-Kin AU - Syn WK AD - Division of Gastroenterology and Hepatology, Saint Louis University, St Louis, Missouri, United States of America; Department of Pathology, Saint Louis University, St Louis, Missouri, United States of America; Department of Physiology, Faculty of Medicine and Nursing, University of Basque Country UPV/EHU, Leioa, Vizcaya, Spain. Electronic address: wingkin.syn@health.slu.edu. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Am J Med Sci JT - The American journal of the medical sciences JID - 0370506 SB - IM OTO - NOTNLM OT - MASLD OT - NHANES OT - aging OT - cardiometabolic risk factors OT - cognition OT - fibrosis OT - memory OT - metabolic burden COIS- Declaration of interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:37 PHST- 2026/07/10 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:37 [entrez] AID - S0002-9629(26)00408-8 [pii] AID - 10.1016/j.amjms.2026.10.009 [doi] PST - aheadofprint SO - Am J Med Sci. 2026 Oct 27:S0002-9629(26)00408-8. doi: 10.1016/j.amjms.2026.10.009. PMID- 42946251 OWN - NLM STAT- Publisher LR - 20261027 IS - 1538-2990 (Electronic) IS - 0002-9629 (Linking) DP - 2026 Oct 27 TI - Clinical Utility of Plasma Microbial Cell-Free DNA Sequencing in Adult Immunocompromised Patients: A Real-World Cohort Study. LID - S0002-9629(26)00406-4 [pii] LID - 10.1016/j.amjms.2026.10.011 [doi] AB - BACKGROUND: Plasma metagenomic next generation sequencing (mNGS) enables noninvasive pathogen detection independent of organism viability. Although analytic performance has been described, the real-world clinical impact in immunocompromised hosts remains incompletely defined. METHODS: We conducted a retrospective cohort study of immunocompromised adults undergoing plasma mNGS testing from January 2022 through November 2024 at the Medical University of South Carolina. Immunocompromised conditions included solid organ transplantation, hematopoietic stem cell transplantation, active hematologic malignancy, HIV, or biologic immunosuppressants. Clinical impact was adjudicated by infectious disease physicians as positive, negative, or neutral. Positive results were classified as definite, probable, possible, or unlikely infectious based on clinical correlation and concordance with conventional microbiologic testing (CMT). RESULTS: Ninety-eight patients were included (median age 60 years [range 18-76]; 63% male), including solid organ transplant (31%), hematopoietic stem cell transplant (8%), HIV (13%), active malignancy (42%), corticosteroids (3%), and biologic therapy (10%). At testing, 85% were receiving antimicrobials and 88% were hospitalized. Plasma mNGS was positive in 62% of cases, with CMT concordance in 30%. Thirty-two percent of tests had positive clinical impact, identifying unrecognized infection or antimicrobial optimization. Sixty-six percent had no treatment impact and 2% had potential negative impact. More than half of discordant positives were deemed possible or unlikely infections. False-negative cases included invasive mold and nontuberculous mycobacterial infections. CONCLUSIONS: In this antimicrobial-exposed immunocompromised cohort, plasma mNGS frequently detected organisms but less often influenced clinical management. Discordant positives were common, and false negatives occurred in selected syndromes, supporting cautious interpretation and stewardship-guided use. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Charles, Drew W AU - Charles DW AD - Division of Infectious Disease, Medical University of South Carolina, Charleston, South Carolina, USA. Electronic address: charlesd@musc.edu. FAU - Alkabab, Yosra AU - Alkabab Y AD - Division of Infectious Disease, Medical University of South Carolina, Charleston, South Carolina, USA. FAU - Sharma, Divisha AU - Sharma D AD - Division of Infectious Disease, Medical University of South Carolina, Charleston, South Carolina, USA. FAU - Adekunle, Ruth O AU - Adekunle RO AD - Division of Infectious Disease, Medical University of South Carolina, Charleston, South Carolina, USA. FAU - Catalano, Jillian AU - Catalano J AD - College of Medicine, Medical University of South Carolina, Charleston, South Carolina. FAU - Colven, Rachel AU - Colven R AD - Department of Pharmacy, Medical University of South Carolina, Charleston, South Carolina, USA. FAU - Curry, Scott R AU - Curry SR AD - Division of Infectious Disease, Medical University of South Carolina, Charleston, South Carolina, USA. FAU - Dorman, Susan E AU - Dorman SE AD - Division of Infectious Disease, Medical University of South Carolina, Charleston, South Carolina, USA. FAU - Meissner, Eric G AU - Meissner EG AD - Division of Infectious Disease, Medical University of South Carolina, Charleston, South Carolina, USA. FAU - Mills, Alexandra AU - Mills A AD - Division of Infectious Disease, Medical University of South Carolina, Charleston, South Carolina, USA. FAU - Patterson, Logan AU - Patterson L AD - Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC, USA. FAU - Teixeria, Charles S AU - Teixeria CS AD - Division of Infectious Disease, Medical University of South Carolina, Charleston, South Carolina, USA. FAU - Tyler, Robert Sean AU - Tyler RS AD - Division of Infectious Disease, Medical University of South Carolina, Charleston, South Carolina, USA. FAU - Salgado, Cassandra D AU - Salgado CD AD - Division of Infectious Disease, Medical University of South Carolina, Charleston, South Carolina, USA. FAU - Harris, Courtney E AU - Harris CE AD - Division of Infectious Disease, Medical University of South Carolina, Charleston, South Carolina, USA. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Am J Med Sci JT - The American journal of the medical sciences JID - 0370506 SB - IM OTO - NOTNLM OT - Karius test OT - immunocompromised OT - next generation sequencing diagnostics OT - plasma microbial cell-free DNA COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: The corresponding author, Drew Charles's sister, Mckenzie Nehaul, is employed by Karius, Inc. as an inside sales representative. The author has received no direct financial benefit from this employment relationship and has had no involvement in the preparation, analysis, or interpretation of data discussed in this manuscript related to Karius. This relationship is disclosed in the interest of full transparency. All other authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:37 PHST- 2026/05/04 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:37 [entrez] AID - S0002-9629(26)00406-4 [pii] AID - 10.1016/j.amjms.2026.10.011 [doi] PST - aheadofprint SO - Am J Med Sci. 2026 Oct 27:S0002-9629(26)00406-4. doi: 10.1016/j.amjms.2026.10.011. PMID- 42946250 OWN - NLM STAT- Publisher LR - 20261027 IS - 1538-2990 (Electronic) IS - 0002-9629 (Linking) DP - 2026 Oct 27 TI - Associations between Global Immune-Nutrition-Inflammation Index and Risk of Mortality in Intensive Care Unit Patients. LID - S0002-9629(26)00405-2 [pii] LID - 10.1016/j.amjms.2026.10.013 [doi] AB - PURPOSE: This retrospective study aimed to evaluate the association between the newly developed Global Immune-Nutrition-Inflammation (GINI) index and the risk of all-cause mortality among patients admitted to the intensive care unit (ICU). METHODS: Data were extracted from the Medical Information Mart for Intensive Care IV version 3.0 (MIMIC-IV-3.0) database. Associations between the GINI index and all-cause mortality were assessed using Kaplan-Meier survival curves and multivariate weighted Cox regression models. Additionally, restricted cubic spline (RCS) analyses were performed to explore potential non-linear relationships between the GINI index and hazard ratios (HRs). Subgroup analyses were conducted to evaluate possible interactions between the GINI index and selected clinical features. RESULTS: A total of 970 patients were included, of whom 11.75% (N = 114) experienced all-cause mortality. A significant association was observed between a higher GINI index and increased risk of all-cause mortality. This association remained consistent when the GINI index was treated as a continuous variable (HR = 1.12; P = 0.03) and when categorized into groups (Group 2 vs. Group 1: HR = 1.97; P = 0.02; Group 3 vs. Group 1: HR = 2.17; P = 0.01). The RCS analysis supported a linear, rather than a non-linear, relationship between the GINI index and all-cause mortality (P > 0.05 for non-linearity). CONCLUSIONS: The GINI index is associated with elevated all-cause mortality risk among ICU patients and may serve as a promising candidate for mortality risk stratification, which highlights the potential benefit of timely targeted intervention. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Li, Guiyuan AU - Li G AD - Department of Urology, Zhuhai People's Hospital (Zhuhai Clinical Medical College of Jinan University), Zhuhai, China. FAU - Wang, Minxing AU - Wang M AD - Department of Neurology, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China. FAU - Zhuo, Shenghua AU - Zhuo S AD - International Center for Aging and Cancer, Hainan Medical University (Hainan Academy of Medical Sciences), Haikou, China. FAU - Feng, Haixiang AU - Feng H AD - College of Traditional Chinese Medicine, Hainan Medical University (Hainan Academy of Medical Sciences), Haikou, China. FAU - Cui, Chenchen AU - Cui C AD - School of Clinical Medicine, Wannan Medical College, Wuhu, Anhui, China. FAU - Zhang, Jinhui AU - Zhang J AD - Department of Intensive Care Unit, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China. FAU - Liu, Zhen AU - Liu Z AD - Department of Neurology, The People's Hospital of Dantu, Zhenjiang, Jiangsu, China. Electronic address: zhenliu3358@163.com. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Am J Med Sci JT - The American journal of the medical sciences JID - 0370506 SB - IM OTO - NOTNLM OT - All-cause Mortality OT - Global Immune-Nutrition-Inflammation Index OT - Intensive Care Unit COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:37 PHST- 2025/09/19 00:00 [received] PHST- 2026/08/15 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:37 [entrez] AID - S0002-9629(26)00405-2 [pii] AID - 10.1016/j.amjms.2026.10.013 [doi] PST - aheadofprint SO - Am J Med Sci. 2026 Oct 27:S0002-9629(26)00405-2. doi: 10.1016/j.amjms.2026.10.013. PMID- 42946237 OWN - NLM STAT- Publisher LR - 20261027 IS - 1549-9642 (Electronic) IS - 1549-9634 (Linking) DP - 2026 Oct 27 TI - Dynamic conductivity model-correlated membrane kinetics with cell viability, permeability, and nanoparticle delivery efficiency. PG - 103044 LID - S1549-9634(26)00145-0 [pii] LID - 10.1016/j.nano.2026.103044 [doi] AB - Pulse settings dominate cell viability and membrane permeability during reversible electroporation for intracellular nanoparticle delivery. This work establishes a dynamic membrane conductivity model to quantitatively resolve transmembrane potential, pore radius, and pore density under varying pulse amplitudes, elucidating quantitative correlations linking pore dynamics to cellular viability, membrane integrity, and permeabilization. The nanoparticle experiments provided an experimental comparison with the field-dependent membrane responses predicted by the model, followed by parameter optimization for A549 lung carcinoma cells. A panel of in vitro characterizations was performed for model verification, including viability quantification, membrane injury assessment, post-treatment DiBAC4(3) fluorescence assessment of membrane depolarization, propidium iodide (PI) permeation assays, and measurements of cell-associated fluorescent nanoparticle signal across an electric-field gradient. Simulations reveal that both pore density and pore size rise with elevated field strength. Crossing a critical threshold triggers saturated pore density and excessive pore dilation, inducing irreversible membrane breakdown and reduced viability, consistent with experimental observations: moderate electric fields promote nanoparticle uptake, whereas overly intense fields cause massive cell death. For A549 cells, five 100 mus pulses at 500-700 V/cm yield an optimal trade-off between delivery efficiency and cell survival; at a fixed nanoparticle concentration of 30 mug/ml, 700 V/cm was selected from the tested field strengths as a candidate operating condition; the concentration had been selected separately at 600 V/cm. A joint optimum was not established. The experimental measurements characterize field-dependent changes in cell viability, membrane permeabilization, and nanoparticle uptake. These measurements do not directly determine absolute membrane pore radii, pore densities, or pore-size distributions and therefore do not independently validate the absolute numerical values predicted by the electroporation model. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Zhang, Naming AU - Zhang N AD - The School of Electrical Engineering, Xi'an Jiaotong University, Xi'an, 710049, China. FAU - Wang, Yuanchao AU - Wang Y AD - The School of Electrical Engineering, Xi'an Jiaotong University, Xi'an, 710049, China. FAU - Li, Bairu AU - Li B AD - The School of Electrical Engineering, Xi'an Jiaotong University, Xi'an, 710049, China. FAU - Ning, Shuya AU - Ning S AD - The School of Electronic Information and Artificial Intelligence, Shaanxi University of Science and Technology, Xi'an, 710021, China. Electronic address: ningshuya@sust.edu.cn. FAU - Wei, Wei AU - Wei W AD - The Comprehensive Breast Care Center, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China. FAU - Wang, Shuhong AU - Wang S AD - The School of Electrical Engineering, Xi'an Jiaotong University, Xi'an, 710049, China. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Nanomedicine JT - Nanomedicine : nanotechnology, biology, and medicine JID - 101233142 SB - IM OTO - NOTNLM OT - Cell membrane OT - Nanoparticle OT - Pulsed electric field intensity OT - Reversible electroporation COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:37 PHST- 2026/07/20 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:37 [entrez] AID - S1549-9634(26)00145-0 [pii] AID - 10.1016/j.nano.2026.103044 [doi] PST - aheadofprint SO - Nanomedicine. 2026 Oct 27:103044. doi: 10.1016/j.nano.2026.103044. PMID- 42946236 OWN - NLM STAT- Publisher LR - 20261027 IS - 1528-8447 (Electronic) IS - 1526-5900 (Linking) DP - 2026 Oct 27 TI - Thermal pain tolerance depends on stimulus duration and thermode type. PG - 106481 LID - S1526-5900(26)00299-3 [pii] LID - 10.1016/j.jpain.2026.106481 [doi] AB - Quantitative Sensory Testing (QST) measures how acute pain and sensation vary within and across individuals. It remains unclear how specific stimulus features, such as thermode type and stimulation duration, affect QST outcomes. We measured acute thermal pain in 93 healthy volunteers using three thermode types and two durations to assess potential impacts on QST outcomes. Across three experiments, participants completed two QST tasks, in which heat was applied to 8 sites on the left forearm. Thermode type (ATS, CHEPS, T03) and stimulus duration (3s, 8s) varied across tasks. We used linear regression to identify pain threshold, tolerance, and temperature-pain correlation (r(2)). Briefer stimuli were associated with higher pain tolerance (t(45) = 5.84, p < 0.001). Thermode type also influenced pain tolerance, such that tolerance was lower with higher surface area (t(45) = 4.79, p < 0.001). There was a weak effect of duration on pain Threshold. Pain threshold was higher in 3s duration than in 8s duration (t(45) = 2.25, p = 0.03). Neither duration nor thermode type affected temperature-pain correlation, and these effects did not differ by controlling for biological sex or task order (all p values > 0.05). These results demonstrate that QST outcomes are differentially sensitive to stimulus features, highlighting the importance of methodological standardization and the careful selection of suprathreshold stimuli in pain experiments. PERSPECTIVE: This study demonstrates the importance of considering stimulus features in "big data" approaches, as pain tolerance is significantly affected by stimulus duration and thermode type, whereas pain thresholds and temperature-pain correlations remain relatively stable. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Wei, Yi AU - Wei Y AD - National Center for Complementary and Integrative Health, National Institutes of Health, Bethesda, MD 20892; Neuroscience and Cognitive Science Program, University of Maryland, College Park, MD 20742. FAU - Storkson, Julie S P AU - Storkson JSP AD - National Center for Complementary and Integrative Health, National Institutes of Health, Bethesda, MD 20892. FAU - Sherwood, Kai AU - Sherwood K AD - National Center for Complementary and Integrative Health, National Institutes of Health, Bethesda, MD 20892. FAU - Delity, Maya Joshi AU - Delity MJ AD - National Center for Complementary and Integrative Health, National Institutes of Health, Bethesda, MD 20892. FAU - Akintola, Titilola AU - Akintola T AD - National Center for Complementary and Integrative Health, National Institutes of Health, Bethesda, MD 20892. FAU - Dildine, Troy C AU - Dildine TC AD - National Center for Complementary and Integrative Health, National Institutes of Health, Bethesda, MD 20892. FAU - Owens, Hayley AU - Owens H AD - National Center for Complementary and Integrative Health, National Institutes of Health, Bethesda, MD 20892. FAU - Atlas, Lauren Y AU - Atlas LY AD - National Center for Complementary and Integrative Health, National Institutes of Health, Bethesda, MD 20892; Neuroscience and Cognitive Science Program, University of Maryland, College Park, MD 20742; National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892; National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD 21224. Electronic address: lauren.atlas@nih.gov. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Pain JT - The journal of pain JID - 100898657 SB - IM OTO - NOTNLM OT - QST OT - acute pain OT - duration OT - healthy volunteers OT - reliability OT - sex differences OT - thermal pain OT - thermode EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:37 PHST- 2026/07/08 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:37 [entrez] AID - S1526-5900(26)00299-3 [pii] AID - 10.1016/j.jpain.2026.106481 [doi] PST - aheadofprint SO - J Pain. 2026 Oct 27:106481. doi: 10.1016/j.jpain.2026.106481. PMID- 42946232 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-3441 (Electronic) IS - 0939-6411 (Linking) DP - 2026 Oct 27 TI - Inhibition of ketoprofen photodecomposition mediated by methyl radicals: ion-pair formation with dibucaine and selection of radical-free patch base materials. PG - 115267 LID - S0939-6411(26)00288-2 [pii] LID - 10.1016/j.ejpb.2026.115267 [doi] AB - Ketoprofen (KP), the most photosensitive non-steroidal anti-inflammatory drug, causes photocontact dermatitis attributed to free radicals generated under irradiation. We examined which radical species KP intercepts and how to limit exposure to them. Of three species generated independently ((*)OH, O(2)(*)(-) and (*)CH(3)), only (*)CH(3) showed a dependence on KP concentration, in both a 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) competition assay and electron spin resonance (ESR) spin trapping with 5,5-dimethyl-1-pyrroline N-oxide (DMPO). Dibucaine (DBC) suppressed KP photodegradation, reducing the rate constant from 5.88 to 3.20 x 10(-2) min(-1), and was more effective than Trolox(R) (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid). Differential scanning calorimetry and nuclear magnetic resonance (NMR) spectroscopy located the interaction at KP's carboxyl group, the site where photodegradation is initiated, with 1:1 stoichiometry, consistent with an ion pair involving hydrophobic hydration. Unlike an antioxidant or a UV absorber, DBC was not consumed under UV irradiation, and unlike an inclusion complex, it engages a single functional group. DBC also reduced the patch release rate constant, so that photostabilization must be balanced against altered release. Styrene-isoprene-styrene copolymer patch bases generated radicals under UV irradiation only in grades containing dibutylhydroxytoluene. Both approaches can therefore limit KP photodegradation. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Takara, Ayaka AU - Takara A AD - Faculty of Pharmaceutical Sciences, Tokyo University of Science, 6-3-1 Niijuku, Katsushika, Tokyo 125-8585 Japan. FAU - Tanada, Reo AU - Tanada R AD - Faculty of Pharmaceutical Sciences, Tokyo University of Science, 6-3-1 Niijuku, Katsushika, Tokyo 125-8585 Japan. FAU - Kobayashi, Kenshiro AU - Kobayashi K AD - Faculty of Pharmaceutical Sciences, Tokyo University of Science, 6-3-1 Niijuku, Katsushika, Tokyo 125-8585 Japan. FAU - Shimada, Yohsuke AU - Shimada Y AD - Faculty of Pharmaceutical Sciences, Tokyo University of Science, 6-3-1 Niijuku, Katsushika, Tokyo 125-8585 Japan; Research Institute for Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510 Japan. FAU - Goto, Satoru AU - Goto S AD - Faculty of Pharmaceutical Sciences, Tokyo University of Science, 6-3-1 Niijuku, Katsushika, Tokyo 125-8585 Japan; Research Institute for Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510 Japan. Electronic address: s.510@rs.tus.ac.jp. FAU - Asada, Mizue AU - Asada M AD - Division of Electronic Properties, Department of Materials Molecular Science, Institute for Molecular Science, National Institutes of Natural Science, Myodaiji, Okazaki, Aichi 444-8585, Japan. FAU - Nakamura, Toshikazu AU - Nakamura T AD - Division of Electronic Properties, Department of Materials Molecular Science, Institute for Molecular Science, National Institutes of Natural Science, Myodaiji, Okazaki, Aichi 444-8585, Japan. FAU - Inoo, Katsuyuki AU - Inoo K AD - Teikoku Seiyaku Co., Ltd., 567 Sanbonmatsu, Higashikagawa, Kagawa 769-2695 Japan. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Eur J Pharm Biopharm JT - European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V JID - 9109778 SB - IM OTO - NOTNLM OT - Dibucaine OT - Ion pair OT - Ketoprofen OT - Methyl radical OT - Photodegradation OT - Spin trapping OT - Transdermal patch COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:37 PHST- 2026/08/21 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:37 [entrez] AID - S0939-6411(26)00288-2 [pii] AID - 10.1016/j.ejpb.2026.115267 [doi] PST - aheadofprint SO - Eur J Pharm Biopharm. 2026 Oct 27:115267. doi: 10.1016/j.ejpb.2026.115267. PMID- 42946227 OWN - NLM STAT- Publisher LR - 20261027 IS - 1464-3405 (Electronic) IS - 0960-894X (Linking) DP - 2026 Oct 27 TI - In vitro activity of tizoxanide derivatives on Trypanosoma cruzi bloodstream trypomastigotes. PG - 130810 LID - S0960-894X(26)00277-5 [pii] LID - 10.1016/j.bmcl.2026.130810 [doi] AB - Chagas disease, which is transmitted by Trypanosoma cruzi parasites, remains a major neglected tropical disease for which there are limited treatment choices. In this study, we present the in vitro evaluation of 31 tizoxanide (TIZ) derivatives against the Y strain of T. cruzi bloodstream trypomastigotes. Several of the compounds exhibited significantly enhanced trypanocidal activity compared to benznidazole (BZ), the reference drug. At 4  degrees C, eight derivatives demonstrated IC(5)(0) values ranging from 25 to 50 muM, up to four times more active than BZ. At physiological temperature (37  degrees C), these compounds exhibited enhanced potency, with IC(5)(0) values as low as 1.9 muM and selectivity indices (SIs) of up to 77, indicating low cytotoxicity to host macrophages. The most promising of these compounds, 13, 19 and 21 exhibited superior efficacy to both BZ and TIZ, the key active metabolite of nitazoxanide (NTZ). In vitro DMPK analyses of selected front runner compounds reveal that halogenation of the phenyl ring provides analogues with significantly increased metabolic stability. Our in vitro findings demonstrate that analogues in this series are potent, metabolically stable and represent potential T. cruzi drug lead candidates, warranting further mechanistic and in vivo evaluation. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved. FAU - Cavalcante, Ana Luiza B G AU - Cavalcante ALBG AD - Instituto Oswaldo Cruz, Fundacao Oswaldo Cruz, Avenida Brasil 4365, CEP 21040-900, Manguinhos, Rio de Janeiro, RJ, Brazil. FAU - Almeida, Renata G AU - Almeida RG AD - Department of Chemistry, Institute of Exact Sciences, Federal University of Minas Gerais, CEP 31270-901, Belo Horizonte, MG, Brazil. FAU - Taujanskas, Joshua AU - Taujanskas J AD - Department of Chemistry, University of Liverpool, Liverpool L69 3BX, United Kingdom. FAU - Pate, Sophie AU - Pate S AD - Department of Chemistry, University of Liverpool, Liverpool L69 3BX, United Kingdom. FAU - Jardim, Guilherme A M AU - Jardim GAM AD - Department of Chemistry, Institute of Exact Sciences, Federal University of Minas Gerais, CEP 31270-901, Belo Horizonte, MG, Brazil. FAU - da Silva Junior, Eufranio N AU - da Silva Junior EN AD - Department of Chemistry, Institute of Exact Sciences, Federal University of Minas Gerais, CEP 31270-901, Belo Horizonte, MG, Brazil. FAU - Bower, John F AU - Bower JF AD - Department of Chemistry, University of Liverpool, Liverpool L69 3BX, United Kingdom. FAU - O'Neill, Paul M AU - O'Neill PM AD - Department of Chemistry, University of Liverpool, Liverpool L69 3BX, United Kingdom. FAU - Menna-Barreto, Rubem F S AU - Menna-Barreto RFS AD - Instituto Oswaldo Cruz, Fundacao Oswaldo Cruz, Avenida Brasil 4365, CEP 21040-900, Manguinhos, Rio de Janeiro, RJ, Brazil. Electronic address: rubemb@ioc.fiocruz.br. FAU - Stachulski, Andrew V AU - Stachulski AV AD - Department of Chemistry, University of Liverpool, Liverpool L69 3BX, United Kingdom. Electronic address: stachuls@liverpool.ac.uk. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Bioorg Med Chem Lett JT - Bioorganic & medicinal chemistry letters JID - 9107377 SB - IM OTO - NOTNLM OT - Benznidazole OT - DMPK properties OT - Thiazolides OT - Tizoxanide OT - Trypanosoma COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:36 PHST- 2026/08/07 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/24 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:36 [entrez] AID - S0960-894X(26)00277-5 [pii] AID - 10.1016/j.bmcl.2026.130810 [doi] PST - aheadofprint SO - Bioorg Med Chem Lett. 2026 Oct 27:130810. doi: 10.1016/j.bmcl.2026.130810. PMID- 42946218 OWN - NLM STAT- Publisher LR - 20261027 IS - 1875-5305 (Electronic) IS - 0929-8665 (Linking) DP - 2026 Oct 27 TI - Effects of Conjugation Strategy on the Anticancer Activity and Selectivity of Melittin Bioconjugates. LID - S0929-8665(26)00044-5 [pii] LID - 10.1016/j.ppl.2026.10.004 [doi] AB - BACKGROUND: Melittin is a membrane-active anticancer peptide with broad cytotoxicity, but its therapeutic application is limited by low tumor selectivity and toxicity toward non-tumor cells. This study evaluated whether conjugation with the CD44-targeting aptamer peptide P7 could modulate melittin activity according to peptide orientation and spacer incorporation. METHODS: BP7Mel-C, BP7Mel-N, and BP7(AEEA)Mel-N were synthesized by Fmoc-based solid-phase peptide synthesis, purified by reverse-phase HPLC, and characterized by mass spectrometry. In silico ADMET and half-life predictions, circular dichroism, fluorescence spectroscopy, serum degradation, flow cytometry, MTT cytotoxicity, clonogenic assays, and membrane permeabilization assays were performed. RESULTS: The bioconjugates retained the alpha-helical conformation associated with melittin membrane interaction, whereas peptide orientation affected predicted and experimental stability. P7-FAM showed greater fluorescence in tumor cell lines than in MRC-5 cells under the tested conditions. Cytotoxicity varied with peptide architecture and cell line. BP7(AEEA)Mel-N showed an IC(50) of 16.02 mug/mL in MRC-5 cells, compared with 5.82 mug/mL for melittin, whereas BP7Mel-N showed an IC(50) of 6.50 mug/mL. In MDA-MB-231 cells, BP7Mel-N showed reduced potency compared with melittin (32.38 vs. 7.74 mug/mL). All peptides induced substantial CF release in POPC vesicles at concentrations corresponding to the MRC-5 IC(50). CONCLUSIONS: P7-melittin conjugation modulated the physicochemical and biological properties of melittin in an architecture- and cell-line-dependent manner. BP7(AEEA)Mel-N showed reduced cytotoxicity toward MRC-5 cells, whereas BP7Mel-N displayed reduced potency in MDA-MB-231 cells. These findings indicate that membrane permeabilization alone does not explain the cellular responses. A key limitation is that targeting, pharmacokinetic predictions, and therapeutic efficacy were not validated in vivo. CI - Copyright (c) 2026 The Authors. Publishing services by Elsevier B.V. All rights reserved. FAU - Nunes, Leticia Oliveira Catarin AU - Nunes LOC AD - Sao Paulo State University (UNESP), Institute of Chemistry, Araraquara, 14800-060, Brazil; Sao Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, 14800-903, Brazil. FAU - Aragao, Ariani Rodrigues AU - Aragao AR AD - Sao Paulo State University (UNESP), Institute of Chemistry, Araraquara, 14800-060, Brazil. FAU - Salvador, Lara Cerazi AU - Salvador LC AD - Sao Paulo State University (UNESP), Institute of Chemistry, Araraquara, 14800-060, Brazil. FAU - Portuondo Fuentes, Deivys Leandro AU - Portuondo Fuentes DL AD - Sao Paulo State University (UNESP), Institute of Chemistry, Araraquara, 14800-060, Brazil. FAU - Navegante, Geovana AU - Navegante G AD - Sao Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, 14800-903, Brazil. FAU - de Oliveira Baracat, Pedro Vinicios AU - de Oliveira Baracat PV AD - Sao Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, 14800-903, Brazil. FAU - Stringhetta, Giulia Rodrigues AU - Stringhetta GR AD - Molecular Oncology Research Center, Barretos Cancer Hospital, 14784-400, Barretos, Brazil. FAU - de Moraes, Rodolfo Osin AU - de Moraes RO AD - Sao Paulo State University (UNESP), School of Agricultural Sciences, Registro, 11900-000, Brazil. FAU - Santiago, Patricia Soares AU - Santiago PS AD - Sao Paulo State University (UNESP), School of Agricultural Sciences, Registro, 11900-000, Brazil. FAU - Silva-Oliveira, Renato Jose AU - Silva-Oliveira RJ AD - Molecular Oncology Research Center, Barretos Cancer Hospital, 14784-400, Barretos, Brazil. FAU - Valente, Valeria AU - Valente V AD - Sao Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, 14800-903, Brazil. FAU - Santos-Filho, Norival Alves AU - Santos-Filho NA AD - Sao Paulo State University (UNESP), Institute of Chemistry, Araraquara, 14800-060, Brazil; Sao Paulo State University (UNESP), School of Pharmaceutical Sciences, Araraquara, 14800-903, Brazil. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Protein Pept Lett JT - Protein and peptide letters JID - 9441434 SB - IM OTO - NOTNLM OT - Anticancer peptides OT - CD44 OT - aptamer peptide OT - bioactive peptide OT - bioconjugation OT - melittin OT - tumor selectivity COIS- Declaration of Competing Interest ☒ The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Leticia Oliveira Catarin Nunes has patent #BR1020230268960 issued to Universidade Estadual Paulista - UNESP. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:36 PHST- 2026/08/04 00:00 [received] PHST- 2026/10/23 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:36 [entrez] AID - S0929-8665(26)00044-5 [pii] AID - 10.1016/j.ppl.2026.10.004 [doi] PST - aheadofprint SO - Protein Pept Lett. 2026 Oct 27:S0929-8665(26)00044-5. doi: 10.1016/j.ppl.2026.10.004. PMID- 42946215 OWN - NLM STAT- Publisher LR - 20261027 IS - 1535-9484 (Electronic) IS - 1535-9476 (Linking) DP - 2026 Oct 27 TI - Plasma proteomics enhances genetic risk prediction and biological understanding of venous thromboembolism. PG - 101692 LID - S1535-9476(26)00188-X [pii] LID - 10.1016/j.mcpro.2026.101692 [doi] AB - BACKGROUND: Venous thromboembolism (VTE) is a leading cause of morbidity and the third most common vascular cause of death globally. While clinical and genetic risk factors have improved VTE risk stratification, they do not fully capture underlying disease biology. We hypothesized that integrating large-scale plasma proteomics with a validated polygenic risk score (PRS(VTE)) would reveal novel biological understanding and improve risk stratification. METHODS: In 44,138 UK Biobank participants, we examined associations between PRS(VTE) and 2,911 plasma proteins using multivariable linear regressions. We evaluated proteins as predictors of incident VTE using Cox regression models. We employed bidirectional Mendelian randomization (MR) using cis-acting protein quantitative trait loci (cis-pQTLs) instruments and genome-wide association summary statistics to infer potential causality. A protein-based risk score (ProteinRS) was developed with Least Absolute Shrinkage and Selection Operator (LASSO) logistic regression and compared against a clinical risk model. RESULTS: PRS(VTE) associated with 265 proteins after false-discovery-rate correction (FDR < 0.01). Pathway enrichment analysis of these proteins highlighted cell adhesion, cell-junction organization, and transmembrane receptor signaling. Time-to-event analyses identified 354 proteins that predicted incident VTE (FDR < 0.01). MR implicated 14 proteins in potential causal pathways to VTE, while genetic liability to VTE affected the levels of 44 proteins. A 60-protein ProteinRS improved discrimination beyond clinical factors (area under the curve (AUC) 0.664 vs 0.647; P = 0.024). Participants above the 80th percentile of ProteinRS had a 49% higher hazard of VTE (hazard ratio (HR) = 1.49, 95% confidence interval (CI): 1.17-1.90) and those below the 20th percentile had a 48% lower hazard (HR = 0.52, 95% CI: 0.34-0.79) relative to the 20-80th percentile. CONCLUSIONS: Integrating genetic and proteomics data uncovered molecular pathways that link genetic susceptibility to VTE, highlighted actionable protein targets, and modestly enhanced risk prediction. Plasma proteomics therefore holds promise for precision prevention of VTE. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Mei, Yingyi AU - Mei Y AD - Cardiac Functional Genomics group, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. FAU - Vad, Oliver Bundgaard AU - Vad OB AD - Cardiac Functional Genomics group, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark; Department of Cardiology, Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark. FAU - Beyer, Astrid Filt AU - Beyer AF AD - Cardiac Functional Genomics group, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. FAU - Ghouse, Jonas AU - Ghouse J AD - Cardiac Functional Genomics group, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark; Dept. of Molecular Medicine (MOMA), Aarhus University Hospital, Aarhus, Denmark; Dept. of Clinical Medicine, Aarhus University, Aarhus, Denmark. FAU - Lundegaard, Pia Rengtved AU - Lundegaard PR AD - Cardiac Functional Genomics group, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. Electronic address: plundegaard@sund.ku.dk. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Mol Cell Proteomics JT - Molecular & cellular proteomics : MCP JID - 101125647 SB - IM OTO - NOTNLM OT - Biomarkers OT - Mendelian Randomization Analysis OT - Proteomics OT - Risk Assessment OT - Venous Thromboembolism COIS- Declaration of Interest Statement ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. ☐ The author is an Editorial Board Member/Editor-in-Chief/Associate Editor/Guest Editor for this journal and was not involved in the editorial review or the decision to publish this article. ☐ The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:36 PHST- 2026/05/28 00:00 [received] PHST- 2026/10/22 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:36 [entrez] AID - S1535-9476(26)00188-X [pii] AID - 10.1016/j.mcpro.2026.101692 [doi] PST - aheadofprint SO - Mol Cell Proteomics. 2026 Oct 27:101692. doi: 10.1016/j.mcpro.2026.101692. PMID- 42946210 OWN - NLM STAT- Publisher LR - 20261027 IS - 2405-4577 (Electronic) IS - 2405-4577 (Linking) DP - 2026 Oct 27 TI - Performance of visual methods for estimating dietary intake in the hospital setting - A systematic review and meta-analysis. PG - 105207 LID - S2405-4577(26)02304-1 [pii] LID - 10.1016/j.clnesp.2026.105207 [doi] AB - BACKGROUND & AIMS: Inadequate dietary intake is one of the main factors contributing to hospital malnutrition, leading to unfavorable clinical outcomes. Visual estimation methods (VEMs) offer a practical alternative for monitoring dietary intake, but their accuracy compared to reference methods remains under debate. This systematic review with meta-analysis aimed to evaluate the performance and accuracy of VEMs in estimating dietary intake in hospitalized patients. METHODS: This study followed the PRISMA guidelines. A comprehensive search was conducted on October 1 and 2, 2025, and subsequently updated on August 5, 2026, using the same search strategy in the PubMed, Scopus, Embase, and Web of Science databases. Two researchers independently selected, extracted, and assessed data quality using the JBI tools (PROSPERO ID: CRD420251151609). Random-effects meta-analyses calculated pooled mean differences with 95% CIs. Intra-subject correlation was accounted for using paired standard errors derived from Bland-Altman limits, directly reported difference, or an imputed mean correlation. Publication bias and sensitivity were evaluated using funnel plots, Egger's test, and leave-one-out analyses. RESULTS: This systematic review included 27 studies on visual tools for estimating dietary intake in hospitalized patients. Of these, 6 were included in the meta-analysis. Publication bias analysis, using Egger's test, did not indicate statistical significance (p = 0.347), providing no evidence of publication bias. Agreement between dietary estimation tools and the reference method demonstrated an overall mean difference (MD) of -14.91 kcal (95% CI: -45.83 to 16.00). The negative MD indicated a trend towards underestimation; however, the pooled effect was not statistically significant. High heterogeneity was observed among studies in the overall analysis (I(2) = 88.4%; p < 0.001). CONCLUSION: The use of visual estimation methods for dietary intake in hospitalized patients, despite a mild tendency toward underestimation, suggests potential utility as a complementary approach for monitoring intake. These tools may offer a practical alternative in daily hospital routines, particularly in clinical settings where routine meal weighing is less feasible to implement. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved. FAU - Rodrigues, Bruno Otavio AU - Rodrigues BO AD - Graduate Program in Nutrition Science, Department of Nutrition and Health, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil. Electronic address: bruno.o.rodrigues@ufv.br. FAU - Figueira, Raquel Lopes AU - Figueira RL AD - Graduate Program in Nutrition Science, Department of Nutrition and Health, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil. FAU - de Oliveira Barbosa Rosa, Carla AU - de Oliveira Barbosa Rosa C AD - Graduate Program in Nutrition Science, Department of Nutrition and Health, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil. Electronic address: carla.rosa@ufv.br. FAU - Ribeiro, Andreia Queiroz AU - Ribeiro AQ AD - Graduate Program in Nutrition Science, Department of Nutrition and Health, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil. FAU - Moreira, Tiago Ricardo AU - Moreira TR AD - Graduate Program in Health Sciences, Department of Medicine and Nursing, Federal University of Vicosa, Vicosa, Minas Gerais, Brazil. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Clin Nutr ESPEN JT - Clinical nutrition ESPEN JID - 101654592 SB - IM OTO - NOTNLM OT - eating OT - hospitalization OT - malnutrition OT - nutrition assessment OT - nutritional requirements OT - visual estimation methods EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:36 PHST- 2026/05/20 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:36 [entrez] AID - S2405-4577(26)02304-1 [pii] AID - 10.1016/j.clnesp.2026.105207 [doi] PST - aheadofprint SO - Clin Nutr ESPEN. 2026 Oct 27:105207. doi: 10.1016/j.clnesp.2026.105207. PMID- 42946205 OWN - NLM STAT- Publisher LR - 20261028 IS - 1873-7064 (Electronic) IS - 0028-3908 (Linking) VI - 303 DP - 2026 Oct 27 TI - Inhibition of the sphingosine-1-phosphate receptor 1 (S1PR1) as a therapeutic target for aromatase inhibitor-induced musculoskeletal symptoms in mice. PG - 111239 LID - S0028-3908(26)00414-4 [pii] LID - 10.1016/j.neuropharm.2026.111239 [doi] AB - Breast cancer (BC) is the leading cause of cancer death among women worldwide with estrogen receptor positive (ER+) BC comprising 70-80% of all cases. Adjuvant therapy with aromatase inhibitors (AIs), such as letrozole (LTZ), is the standard of care in ER + BC patients to prevent recurrence but many patients cease treatment prematurely due to the development of musculoskeletal side effects (i.e. arthralgia, myalgia, decreased grip strength) referred to as aromatase inhibitor-associated musculoskeletal syndrome (AIMSS). The lack of efficacious treatments available for AIMSS and premature treatment cessation being linked to increased patient mortality highlights the critical importance of identifying new therapeutic targets for its management. Here, we directly link LTZ treatment with increased levels of sphingosine-1-phosphate receptor 1 (S1PR1) endogenous ligands in spinal cord tissue of female ovariectomized (OVX) ICR mice. We therefore investigated the impact of reducing S1PR1 activity as a potential therapeutic strategy for alleviating AIMSS-related behaviors. In C57BL/6J OVX female mice, we found that pharmacological inhibition using the selective and competitive S1PR1 antagonist, NIBR-0213, effectively alleviated AIMSS-like behaviors after acute and repeated dosing regimens, with limited therapeutic effectiveness when given prior to LTZ treatment. In a conditioned place preference (CPP) paradigm, LTZ- but not vehicle-treated animals preferred the NIBR-0213 paired chamber, unmasking the presence of a tonic-aversive state driven by LTZ treatment. These findings identify S1PR1 as an important mediator to AIMSS and suggest that S1PR1 antagonists may be most effective as acute interventions to provide relief from the sensory and affective burden of AIMSS. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Herz, Sara M AU - Herz SM AD - Department of Pharmacology/Toxicology, Virginia Commonwealth University, Richmond, VA, USA. Electronic address: herzsm@vcu.edu. FAU - Khan, Abrar M AU - Khan AM AD - Department of Pharmacology/Toxicology, Virginia Commonwealth University, Richmond, VA, USA. FAU - Betancourt-Toscano, Isis AU - Betancourt-Toscano I AD - Department of Pharmacology/Toxicology, Virginia Commonwealth University, Richmond, VA, USA. FAU - Tiron, Caleb G AU - Tiron CG AD - Department of Pharmacology/Toxicology, Virginia Commonwealth University, Richmond, VA, USA. FAU - Ton, Viviane N AU - Ton VN AD - Department of Pharmacology/Toxicology, Virginia Commonwealth University, Richmond, VA, USA. FAU - Selley, Dana E AU - Selley DE AD - Department of Pharmacology/Toxicology, Virginia Commonwealth University, Richmond, VA, USA. FAU - Damaj, M Imad AU - Damaj MI AD - Department of Pharmacology/Toxicology, Virginia Commonwealth University, Richmond, VA, USA; Translational Research Initiative for Pain and Neuropathy, Medical College of Virginia Campus, Virginia Commonwealth University, Richmond, VA, USA. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Neuropharmacology JT - Neuropharmacology JID - 0236217 SB - IM OTO - NOTNLM OT - AIMSS OT - Aromatase inhibitors OT - Breast cancer OT - Letrozole OT - Musculoskeletal pain OT - S1PR1 COIS- Declaration of competing interest The authors declare no conflict of interest. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:36 PHST- 2026/07/07 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 20:36 [entrez] AID - S0028-3908(26)00414-4 [pii] AID - 10.1016/j.neuropharm.2026.111239 [doi] PST - aheadofprint SO - Neuropharmacology. 2026 Oct 27;303:111239. doi: 10.1016/j.neuropharm.2026.111239. PMID- 42946187 OWN - NLM STAT- Publisher LR - 20261027 IS - 1096-0953 (Electronic) IS - 0013-9351 (Linking) DP - 2026 Oct 27 TI - Surface-engineered nanostructured adsorbents for CO(2) capture: From material design to process integration and industrial deployment. PG - 125970 LID - S0013-9351(26)02302-9 [pii] LID - 10.1016/j.envres.2026.125970 [doi] AB - The growing necessity to curb CO(2) emissions from man-made sources has driven the development of highly efficient and economical approaches to carbon capture. The current review is intended to give an overview and critical analysis of nanomaterial-based adsorbents for CO(2) capture with specific consideration of the connections between nanomaterial properties, adsorption processes, process integration, and industrial application. As opposed to the majority of literature surveys, which concentrate on either certain classes of materials or adsorption capacity of various materials separately, the review proposes a holistic view by combining information on the correlations of pore structure, surface chemistry, thermodynamics, adsorption kinetics, selectivity principles, and regeneration behavior of different nanostructured adsorbents, such as metal-organic frameworks (MOFs), covalent organic frameworks (COFs), hierarchical nanoporous carbons, graphene analogs, silica hybrids, metal oxides, and bioinspired materials. Special focus is paid to surface engineering of adsorbents, including amine grafting, heteroatom doping, and compositing, and its influence on CO(2) affinity, cyclic stability, and transport properties of adsorbents. Apart from the lab-scale efficiency, it analyzes issues of scale-up, regeneration efficiency, process intensification, techno-economic viability, and life-cycle sustainability. New possibilities such as machine-learning aided design of new adsorbents, standardized performance measures, and the concept of circular economy are also discussed. This review acts as a guideline and framework for designing nanostructured adsorbents that can be used in carbon management on a large scale. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Baba, Isaac Alhamdu AU - Baba IA AD - Department of Chemical Engineering, Federal University of Technology, PMB. 65, Gidan Kwano Campus, Minna, Niger State, Nigeria. Electronic address: isaac.pg2116629@st.futminna.edu.ng. FAU - Mustapha, Saheed AU - Mustapha S AD - Department of Chemistry, Federal University of Technology, PMB. 65, Gidan Kwano Campus, Minna, Niger State, Nigeria. FAU - Awe, Omoniyi Babajide AU - Awe OB AD - Department of Chemistry & Biochemistry, Bowman-Oddy Laboratories, Room BO 2022, Mail Stop 602, The University of Toledo, 2801 W. Bancroft Street, Toledo, OH 43606, USA. FAU - Abdulkareem, Ambali Saka AU - Abdulkareem AS AD - Department of Chemical Engineering, Federal University of Technology, PMB. 65, Gidan Kwano Campus, Minna, Niger State, Nigeria. FAU - Tijani, Jimoh Oladejo AU - Tijani JO AD - Department of Chemistry, Federal University of Technology, PMB. 65, Gidan Kwano Campus, Minna, Niger State, Nigeria. FAU - Alli, Yakubu Adekunle AU - Alli YA AD - Department of Chemistry, Nelson Mandela University, Port Elizabeth, 6031, South Africa. Electronic address: s230215459@mandela.ac.za. FAU - Obayomi, Kehinde Shola AU - Obayomi KS AD - Institute for Sustainable Industries and Liveable Cities, Victoria University, PO Box 14428, Melbourne, Victoria 8001, Australia. Electronic address: obayomikehindeshola@gmail.com. FAU - Zhang, Jianhua AU - Zhang J AD - Institute for Sustainable Industries and Liveable Cities, Victoria University, PO Box 14428, Melbourne, Victoria 8001, Australia. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Netherlands TA - Environ Res JT - Environmental research JID - 0147621 SB - IM OTO - NOTNLM OT - Air CO(2) capture OT - Industrial scalability OT - Nanostructured adsorbents OT - Surface engineering OT - Techno-economic analysis COIS- Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:36 PHST- 2026/02/26 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:36 [entrez] AID - S0013-9351(26)02302-9 [pii] AID - 10.1016/j.envres.2026.125970 [doi] PST - aheadofprint SO - Environ Res. 2026 Oct 27:125970. doi: 10.1016/j.envres.2026.125970. PMID- 42946185 OWN - NLM STAT- Publisher LR - 20261027 IS - 1096-0384 (Electronic) IS - 0003-9861 (Linking) DP - 2026 Oct 27 TI - USP18 promotes colorectal cancer cell proliferation and migration in association with KRT16 stabilization. PG - 111025 LID - S0003-9861(26)00298-5 [pii] LID - 10.1016/j.abb.2026.111025 [doi] AB - BACKGROUND: The ubiquitin-specific peptidase 18 (USP18) has been implicated in colorectal cancer (CRC), but its downstream effector molecules remain incompletely defined. METHODS: USP18-interacting proteins were identified by immunoprecipitation-mass spectrometry (IP-MS) and validated by co-immunoprecipitation (co-IP). The regulation of KRT16 by USP18 was examined by qPCR, Western blotting, ubiquitination assays and cycloheximide (CHX) chase experiments in SW480 cells. Functional assays were performed in SW480, HT-29 and LoVo cells, comprising CCK-8 proliferation and wound-healing assays in all three cell lines, Transwell migration assays in SW480 cells, and Transwell invasion assays in HT-29 and LoVo cells. Clinical relevance was evaluated in the TCGA and GSE39582 cohorts and in a 100-case hospital cohort by immunohistochemistry (IHC) and paired Western blotting. RESULTS: IP-MS identified KRT16 as a USP18-associated protein. USP18 associated with endogenous KRT16, reduced KRT16 ubiquitination and prolonged the half-life of KRT16 protein. KRT16 and USP18 were concurrently upregulated in CRC in both the TCGA and GSE39582 cohorts; KRT16 expression correlated with USP18 expression (R = 0.68, P < 2.2 x 10-16), discriminated tumor from normal tissue (AUC = 0.899; 95% CI 0.852-0.945) and indicated poor overall survival (KRT16: HR = 2.024, 95% CI 1.273-3.218, P = 0.0029; externally validated in GSE39582, P = 0.0012). In the hospital cohort, high KRT16 expression (61.0%) was associated with T stage, and paired Western blots (n = 14) confirmed coordinated upregulation of USP18 and KRT16. Functionally, KRT16 knockdown reduced proliferation and impaired wound closure across all three cell lines, reduced Transwell migration in SW480 cells, and reduced invasion in HT-29 and LoVo cells, thereby attenuating the pro-tumor effects of USP18 overexpression. CONCLUSION: USP18 stabilizes KRT16 protein through deubiquitination and thereby promotes CRC cell proliferation and migration across multiple cellular backgrounds. These findings support the USP18-KRT16 axis as a candidate molecular axis warranting further preclinical evaluation in CRC. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Zhang, Lin AU - Zhang L AD - Affiliated Hospital of Jiaxing University, The First Hospital of Jiaxing, No. 1882, South Zhonghuan Road, Nanhu District, Jiaxing City, Zhejiang Province 314001, China. FAU - Li, Xin AU - Li X AD - Affiliated Hospital of Jiaxing University, The First Hospital of Jiaxing, No. 1882, South Zhonghuan Road, Nanhu District, Jiaxing City, Zhejiang Province 314001, China. FAU - Yang, Hongjie AU - Yang H AD - Affiliated Hospital of Jiaxing University, The First Hospital of Jiaxing, No. 1882, South Zhonghuan Road, Nanhu District, Jiaxing City, Zhejiang Province 314001, China. FAU - Guo, Zhiqin AU - Guo Z AD - Affiliated Hospital of Jiaxing University, The First Hospital of Jiaxing, No. 1882, South Zhonghuan Road, Nanhu District, Jiaxing City, Zhejiang Province 314001, China. FAU - Chen, Xiangyi AU - Chen X AD - Affiliated Hospital of Jiaxing University, The First Hospital of Jiaxing, No. 1882, South Zhonghuan Road, Nanhu District, Jiaxing City, Zhejiang Province 314001, China. Electronic address: socxy@163.com. FAU - Wu, Wanxin AU - Wu W AD - Affiliated Hospital of Jiaxing University, The First Hospital of Jiaxing, No. 1882, South Zhonghuan Road, Nanhu District, Jiaxing City, Zhejiang Province 314001, China. Electronic address: wuwanxindyh@sina.com. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Arch Biochem Biophys JT - Archives of biochemistry and biophysics JID - 0372430 SB - IM OTO - NOTNLM OT - Colorectal cancer OT - Deubiquitination OT - KRT16 OT - Migration OT - Proliferation OT - USP18 COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:36 PHST- 2026/04/15 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:36 [entrez] AID - S0003-9861(26)00298-5 [pii] AID - 10.1016/j.abb.2026.111025 [doi] PST - aheadofprint SO - Arch Biochem Biophys. 2026 Oct 27:111025. doi: 10.1016/j.abb.2026.111025. PMID- 42946183 OWN - NLM STAT- Publisher LR - 20261027 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) DP - 2026 Oct 27 TI - Transcriptomics-informed phenotypic screening of Rhodiola sachalinensis identifies herbacetin as a cardioprotective candidate against adverse cardiac remodeling. PG - 122582 LID - S0378-8741(26)01437-6 [pii] LID - 10.1016/j.jep.2026.122582 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: Rhodiola sachalinensis Boriss. (Crassulaceae) is a traditional Chinese medicinal plant whose roots and rhizomes are used to invigorate qi and promote blood circulation and have been documented for the management of qi deficiency and blood stasis, chest pain, and angina. Consistent with these traditional uses, modern pharmacological studies have reported antioxidant, anti-inflammatory, and cardiovascular protective activities of R. sachalinensis. However, owing to its complex chemical composition, the bioactive constituents contributing to its cardioprotective effects remain poorly defined. AIM OF THE STUDY: This study aimed to identify cardioprotective constituents of Rhodiola sachalinensis oral solution (RS) through an integrated transcriptomics-informed phenotypic screening strategy and to elucidate the potential mechanisms underlying their effects on cardiac remodeling. MATERIALS AND METHODS: The protective effects of RS were evaluated in an isoproterenol (ISO)-induced cardiac remodeling model, followed by cardiac transcriptomic profiling. Transcriptomic signatures were then integrated with complementary phenotypic assays of phenylephrine-induced cardiomyocyte hypertrophy and transforming growth factor-beta1-induced cardiac fibroblast activation to screen a library of 101 candidate constituents. The prioritized compound was further evaluated in ISO-induced cardiac remodeling and myocardial infarction (MI) models. RESULTS: Transcriptomic profiling of RS-treated hearts identified disease-associated signatures that informed the selection of complementary phenotypic assays for cardiomyocyte hypertrophy and fibroblast activation. Screening of 101 candidate constituents prioritized herbacetin (HBT), a flavonoid showing dual activity in both assays. In vivo, HBT attenuated ISO-induced cardiac dysfunction, cardiomyocyte enlargement, and myocardial fibrosis. These effects were associated with improved mitochondrial ultrastructure, preservation of mitochondrial network integrity, and restoration of ISO-suppressed OPA1 expression. In a complementary MI model, HBT improved cardiac function and reduced adverse remodeling. Mass cytometry (CyTOF) and immunofluorescence analyses further showed that HBT treatment was associated with reduced CCR2-positive inflammatory macrophage features and increased MRC1-positive reparative macrophage features during the early post-MI response. CONCLUSIONS: This study establishes a transcriptomics-informed phenotypic screening strategy for identifying bioactive constituents of RS and identifies HBT as a cardioprotective flavonoid constituent. HBT exhibited protective effects across complementary models of cardiac remodeling, with additional evidence of mitochondrial preservation and altered post-infarction macrophage-associated features in the respective experimental settings. These findings expand the cardiovascular pharmacological understanding of R. sachalinensis and provide a framework for discovering bioactive constituents from complex medicinal plants. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Wang, Yingchao AU - Wang Y AD - School of Pharmacy, Zhejiang University, Hangzhou, 310058, China; Innovation Institute for Artificial Intelligence in Medicine, Zhejiang University, Hangzhou, 310018, China. FAU - Situ, Qiantong AU - Situ Q AD - School of Pharmacy, Zhejiang University, Hangzhou, 310058, China. FAU - Hou, Xiaolu AU - Hou X AD - School of Pharmacy, Zhejiang University, Hangzhou, 310058, China. FAU - Hua, Shuyang AU - Hua S AD - Hangzhou Huawei Pharmaceutical Co., Ltd., Hangzhou 311400, China. FAU - Wang, Yi AU - Wang Y AD - School of Pharmacy, Zhejiang University, Hangzhou, 310058, China; Innovation Institute for Artificial Intelligence in Medicine, Zhejiang University, Hangzhou, 310018, China. FAU - Zhang, Shujing AU - Zhang S AD - School of Pharmacy, Zhejiang University, Hangzhou, 310058, China; Innovation Institute for Artificial Intelligence in Medicine, Zhejiang University, Hangzhou, 310018, China. Electronic address: 11619025@zju.edu.cn. LA - eng PT - Journal Article DEP - 20261027 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 SB - IM OTO - NOTNLM OT - Herbacetin OT - Rhodiola sachalinensis OT - cardiac remodeling OT - macrophages OT - mitochondrial homeostasis OT - phenotypic screening COIS- Declaration of Competing Interest Shuyang Hua is an employee of Hangzhou Huawei Pharmaceutical Co., Ltd., which provided the Rhodiola sachalinensis oral solution for this study. The other authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:36 PHST- 2026/08/13 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:36 [entrez] AID - S0378-8741(26)01437-6 [pii] AID - 10.1016/j.jep.2026.122582 [doi] PST - aheadofprint SO - J Ethnopharmacol. 2026 Oct 27:122582. doi: 10.1016/j.jep.2026.122582. PMID- 42946182 OWN - NLM STAT- Publisher LR - 20261027 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) DP - 2026 Oct 27 TI - Integrated Analysis of the Blood-Absorbed and Gastric Tissue-Distributed Components of Huangqi Fuling Decoction Reveals the Inhibitory Effect on Gastric Cancer Liver Metastasis via the PRMT1/FOXO1 Axis. PG - 122560 LID - S0378-8741(26)01415-7 [pii] LID - 10.1016/j.jep.2026.122560 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: Huangqi Fuling Decoction (HQFLT) is a classic formula that has shown therapeutic potential against gastric cancer (GC) in clinical practice. However, its components and underlying mechanisms remain unclear. AIM OF THE STUDY: This study aimed to investigate the inhibitory effect of HQFLT on liver metastasis of GC and to elucidate its potential pharmacological basis and underlying mechanisms. MATERIALS AND METHODS: UPLC-MS was used to detect the absorbed components in blood and gastric tissues from mice administered intragastrically with HQFLT. WGCNA, LASSO, SVM-RFE, and random forest were applied to screen for HQFLT targets against GC, and the core target was validated in patient tissues. Downstream proteins interacting with the core target were predicted by the STRING database, and the interactions were further analyzed by molecular dynamics simulations. CCK-8, wound healing, Transwell, and rescue assays were performed to evaluate the effects of HQFLT on GC cells. In vivo validation was conducted using a mouse hematogenous colonization model with bioluminescence imaging, H&E, and IHC staining. RESULTS: UPLC-MS identified 128 components in blood and 188 in gastric tissues, with 65 common components of HQFLT detected in both blood and gastric tissues. Machine learning identified protein arginine methyltransferase 1 (PRMT1) as a core target of HQFLT, with elevated expression in GC tissues, and Forkhead box protein O1 (FOXO1) as its downstream target. Inhibition of PRMT1 or FOXO1 suppressed cell migration and invasion, while rescue assays showed that HQFLT exerted its effects partially through regulating the PRMT1/FOXO1 axis. In vivo, HQFLT inhibited liver metastasis of GC, as evidenced by reduced bioluminescence signals, and counteracted the pro-metastatic effect of PRMT1 expression inducer. CONCLUSIONS: This study elucidated the potential pharmacological basis of HQFLT and suggested that its inhibitory effect on liver metastasis of GC was partially mediated through the PRMT1/FOXO1 axis. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Lu, Doudou AU - Lu D AD - School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia, China; Key Laboratory of Dryness Syndrome in Chinese Medicine, Ministry of Education, Ningxia Medical University, Yinchuan, Ningxia, China. FAU - Jia, Shumin AU - Jia S AD - Key Laboratory of Dryness Syndrome in Chinese Medicine, Ministry of Education, Ningxia Medical University, Yinchuan, Ningxia, China. FAU - Song, Chuang AU - Song C AD - College of Pharmacy, Ningxia Medical University, Yinchuan, Ningxia, China; Key Laboratory of Protection, Development and Utilization of Medicinal Resources in Liupanshan Area (Ningxia Medical University), Ministry of Education, Yinchuan, Ningxia, China. FAU - Liu, Wenjing AU - Liu W AD - Key Laboratory of Dryness Syndrome in Chinese Medicine, Ministry of Education, Ningxia Medical University, Yinchuan, Ningxia, China. FAU - Wang, Ruinan AU - Wang R AD - Key Laboratory of Dryness Syndrome in Chinese Medicine, Ministry of Education, Ningxia Medical University, Yinchuan, Ningxia, China. FAU - Zhao, Jianan AU - Zhao J AD - Traditional Chinese Medicine College, Ningxia Medical University, Yinchuan, Ningxia, China. FAU - Feng, Juju AU - Feng J AD - Department of Gastroenterology, Yulin Hospital of Traditional Chinese Medicine, Yulin, Shanxi, China. FAU - Liu, Yu AU - Liu Y AD - Department of Gastroenterology, Yulin Hospital of Traditional Chinese Medicine, Yulin, Shanxi, China. FAU - Qu, Haiyan AU - Qu H AD - Department of Gastroenterology, Yulin Hospital of Traditional Chinese Medicine, Yulin, Shanxi, China. Electronic address: 1275666741@qq.com. FAU - Nan, Yi AU - Nan Y AD - Key Laboratory of Dryness Syndrome in Chinese Medicine, Ministry of Education, Ningxia Medical University, Yinchuan, Ningxia, China; Ningxia Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Regional High-Incidence Diseases, Ningxia Medical University, Yinchuan, Ningxia, China. Electronic address: 20080011@nxmu.edu.cn. FAU - Yuan, Ling AU - Yuan L AD - College of Pharmacy, Ningxia Medical University, Yinchuan, Ningxia, China; Key Laboratory of Protection, Development and Utilization of Medicinal Resources in Liupanshan Area (Ningxia Medical University), Ministry of Education, Yinchuan, Ningxia, China. Electronic address: 20080017@nxmu.edu.cn. LA - eng PT - Journal Article DEP - 20261027 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 SB - IM OTO - NOTNLM OT - FOXO1 OT - Huangqi Fuling Decoction OT - PRMT1 OT - gastric cancer OT - liver metastasis COIS- Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:36 PHST- 2026/08/13 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:36 [entrez] AID - S0378-8741(26)01415-7 [pii] AID - 10.1016/j.jep.2026.122560 [doi] PST - aheadofprint SO - J Ethnopharmacol. 2026 Oct 27:122560. doi: 10.1016/j.jep.2026.122560. PMID- 42946180 OWN - NLM STAT- Publisher LR - 20261027 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) DP - 2026 Oct 27 TI - Total polyphenols from Hemerocallis citrina Baroni leaves improve sleep quality in mice with perimenopausal insomnia and are associated with altered expression of 5-HT(1A)R, ADCY5, PKA, and GABRA2. PG - 122577 LID - S0378-8741(26)01432-7 [pii] LID - 10.1016/j.jep.2026.122577 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: Hemerocallis citrina Baroni has a long history of dietary and medicinal use in China. Its dried whole herb is documented as an ethnic medicinal material in the 2003 edition of the Guizhou Provincial Quality Standards for Chinese Medicinal and Ethnic Medicinal Materials, while Hemerocallis flowers are traditionally used for "ye shao an mei" (), referring to reduced or disturbed sleep at night. Modern studies have demonstrated sleep-improving effects of the flowers, and previous work showed neuroprotective and antidepressant-like activities of total polyphenols from Hemerocallis citrina Baroni leaves (HLTP). However, the effects of HLTP on perimenopausal insomnia (PMI) remain unclear. AIM OF THE STUDY: To evaluate the effects of HLTP on sleep disturbances in mice exhibiting perimenopausal and insomnia-like features and investigate associated molecular changes. MATERIALS AND METHODS: HLTP was prepared using a previously reported procedure. Network pharmacology predicted candidate targets and pathways for 32 phenolic constituents identified in an earlier HLTP batch, while molecular docking assessed predicted binding to 10 core targets selected by protein-protein interaction (PPI) analysis. A composite mouse model was generated using 4-vinylcyclohexene dioxide (VCD) to induce perimenopausal features, followed by para-chloro-DL-phenylalanine (PCPA) to induce insomnia-like disturbances. Seventy female C57BL/6J mice were randomized into seven groups (n = 10 per group): Control, Con+HLTP, Model, HLTP-L/M/H (10, 20, and 40 mg/kg, respectively), and zopiclone. Treatment began with model induction. Behavioral tests, pentobarbital-induced sleep testing, 24 h electroencephalographic/electromyographic (EEG/EMG) polysomnography, histopathology, biochemical assays, and Western blotting were used to evaluate pharmacological effects and associated molecular changes. 16S rRNA sequencing, serum untargeted metabolomics, and brain transcriptomics were additionally performed to characterize complementary changes in the gut microbiota, systemic metabolism, and central gene expression. RESULTS: Network pharmacology prioritized the serotonergic synapse pathway for further investigation. HLTP improved behavioral and sleep-related abnormalities, with the most consistent effects at the high dose. EEG/EMG polysomnography showed a partial shift in sleep-wake distribution toward the Control pattern. During the inactive phase, high-dose HLTP shortened non-rapid eye movement (NREM) and rapid eye movement (REM) sleep latencies, reduced Wake bout number and NREM-to-Wake transitions, prolonged NREM bout duration, and increased NREM sleep time (P < 0.05). HLTP also attenuated neuronal morphological abnormalities and improved sex hormone, monoamine neurotransmitter, oxidative stress, and inflammatory indicators. Western blotting showed increased expression of the 5-hydroxytryptamine 1A receptor (5-HT(1A)R) and gamma-aminobutyric acid type A receptor alpha2 subunit (GABRA2), together with decreased expression of adenylyl cyclase 5 (ADCY5) and protein kinase A (PKA) after HLTP treatment. Multi-omics analyses revealed changes in gut microbial composition, serum metabolism, and brain gene expression following HLTP treatment. Pathway enrichment analysis of exploratory candidate metabolites identified FDR-supported enrichment of steroid biosynthesis. Brain transcriptomics identified FDR-supported enrichment of circadian rhythm in the Model vs. Control comparison and neuroactive ligand-receptor interaction in the HLTP-H vs. Model comparison. None of the tested cross-omics correlations remained significant after FDR correction. CONCLUSIONS: HLTP ameliorated sleep disturbances in mice subjected to the VCD+PCPA composite model, accompanied by improvements in neuroendocrine indicators and changes in 5-HT(1A)R, ADCY5, PKA, and GABRA2 protein expression. The omics findings provided complementary exploratory evidence, although the functional relationships among these changes remain to be established. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Zhang, Zeyu AU - Zhang Z AD - School of Pharmacy, Lanzhou University, 199 West Donggang Road, Lanzhou 730000, China; Codonopsis Radix Industrial Technology Engineering Research Center, 199 West Donggang Road, Lanzhou 730000, China. FAU - Wang, Yanping AU - Wang Y AD - School of Pharmacy, Lanzhou University, 199 West Donggang Road, Lanzhou 730000, China; Codonopsis Radix Industrial Technology Engineering Research Center, 199 West Donggang Road, Lanzhou 730000, China. FAU - Meng, Yanan AU - Meng Y AD - School of Pharmacy, Lanzhou University, 199 West Donggang Road, Lanzhou 730000, China; Codonopsis Radix Industrial Technology Engineering Research Center, 199 West Donggang Road, Lanzhou 730000, China. FAU - Liu, Junhui AU - Liu J AD - School of Pharmacy, Lanzhou University, 199 West Donggang Road, Lanzhou 730000, China; Codonopsis Radix Industrial Technology Engineering Research Center, 199 West Donggang Road, Lanzhou 730000, China. FAU - Shao, Yazhou AU - Shao Y AD - School of Pharmacy, Lanzhou University, 199 West Donggang Road, Lanzhou 730000, China; Codonopsis Radix Industrial Technology Engineering Research Center, 199 West Donggang Road, Lanzhou 730000, China. FAU - Jing, Yichen AU - Jing Y AD - School of Pharmacy, Lanzhou University, 199 West Donggang Road, Lanzhou 730000, China; Codonopsis Radix Industrial Technology Engineering Research Center, 199 West Donggang Road, Lanzhou 730000, China. FAU - Liang, Wenxin AU - Liang W AD - School of Pharmacy, Lanzhou University, 199 West Donggang Road, Lanzhou 730000, China; Codonopsis Radix Industrial Technology Engineering Research Center, 199 West Donggang Road, Lanzhou 730000, China. FAU - Li, Yile AU - Li Y AD - School of Pharmacy, Lanzhou University, 199 West Donggang Road, Lanzhou 730000, China; Codonopsis Radix Industrial Technology Engineering Research Center, 199 West Donggang Road, Lanzhou 730000, China. FAU - Cui, Fang AU - Cui F AD - School of Pharmacy, Lanzhou University, 199 West Donggang Road, Lanzhou 730000, China; Codonopsis Radix Industrial Technology Engineering Research Center, 199 West Donggang Road, Lanzhou 730000, China. FAU - Li, Yun AU - Li Y AD - School of Pharmacy, Gansu University of Chinese Medicine, 34 East Dingxi Road, Lanzhou 730000, China. FAU - Shao, Yufeng AU - Shao Y AD - Departments of Neuroscience, Anatomy, Histology, and Embryology, Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, 199 West Donggang Road, Lanzhou 730000, China. FAU - Hu, Fangdi AU - Hu F AD - School of Pharmacy, Lanzhou University, 199 West Donggang Road, Lanzhou 730000, China; Codonopsis Radix Industrial Technology Engineering Research Center, 199 West Donggang Road, Lanzhou 730000, China; Lanzhou University Shenzhen Research Institute Traditional Chinese Medicine Innovation Center, 98 Shenyuan Road, Shenzhen 518000, China. Electronic address: hufd@lzu.edu.cn. LA - eng PT - Journal Article DEP - 20261027 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 SB - IM OTO - NOTNLM OT - EEG/EMG polysomnography OT - Hemerocallis citrina Baroni leaves OT - Multi-omics analysis OT - Perimenopausal insomnia OT - Serotonergic signaling OT - Total polyphenols COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:36 PHST- 2026/08/07 00:00 [received] PHST- 2026/10/21 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:36 [entrez] AID - S0378-8741(26)01432-7 [pii] AID - 10.1016/j.jep.2026.122577 [doi] PST - aheadofprint SO - J Ethnopharmacol. 2026 Oct 27:122577. doi: 10.1016/j.jep.2026.122577. PMID- 42946171 OWN - NLM STAT- Publisher LR - 20261027 IS - 2949-8759 (Electronic) IS - 2949-8759 (Linking) DP - 2026 Oct 27 TI - Outpatient versus inpatient detoxification of alcohol use disorder: an observational cohort study. PG - 210144 LID - S2949-8759(26)00264-X [pii] LID - 10.1016/j.josat.2026.210144 [doi] AB - INTRODUCTION: Alcohol detoxification can take place in outpatient or inpatient settings, however, clear criteria for allocation are lacking. This study compared patient characteristics between settings, identified predictors of treatment allocation, and compared treatment completion, relapse, adverse events, and costs. METHODS: This prospective cohort study included 75 patients with severe alcohol use disorder (AUD) between December 2022 and January 2024 (41 inpatient, 34 outpatient). Data collection covered demographic and medical characteristics. Multivariable logistic regression with forward stepwise selection (p < 0.1) identified predictors of treatment allocation. Secondary outcomes were treatment completion, relapse, adverse events, a composite 'any adverse outcome', and treatment costs. RESULTS: Gender, marital status, psychiatric comorbidity, and a history of seizures or delirium differed significantly between the groups. A predictive model based solely on demographic characteristics (gender, marital status, and country of origin) achieved an area under the curve (AUC) of 0.789, outperforming a model based on medical characteristics (AUC 0.724) and a mixed model (AUC 0.766). Non-completion did not differ significantly between outpatient and inpatient detoxification (17.6% vs 14.6%; adjusted OR 0.591, p = 0.446), nor did the composite outcome (17.6% vs 24.4%; adjusted OR 1.06, p = 0.94). Relapse occurred only in the outpatient group (n = 6) and adverse events only in the inpatient group (n = 4). The average total cost of outpatient treatment was euro3123 (SD +/- euro1621), compared with euro6681 (SD +/- euro2200) for inpatient treatment. CONCLUSIONS: Demographic factors appear to influence the allocation of detoxification setting more than medical factors, even though the Dutch national protocol lists predominantly medical criteria. While treatment completion did not differ significantly between settings, outpatient detoxification cost about half as much as inpatient detoxification. Relapses and adverse events did not occur frequently enough to draw conclusions about safety. Larger studies in more diverse populations should confirm these findings. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Kieviet, Sicco AU - Kieviet S AD - 'Jellinek, High Care Detox' Department, Vlaardingenlaan 5, 1059GL Amsterdam, the Netherlands. Electronic address: kieviet@hotmail.com. FAU - Zuidmeer, Ruud AU - Zuidmeer R AD - 'Jellinek Outreaching Team', Domselaerstraat 126, 1093 MB, Amsterdam, the Netherlands. Electronic address: Ruud.Zuidmeer@jellinek.nl. FAU - Lijftogt, Niki AU - Lijftogt N AD - 'Jellinek, High Care Detox' Department, Vlaardingenlaan 5, 1059GL Amsterdam, the Netherlands. Electronic address: niki.lijftogt@jellinek.nl. FAU - de Vries, Marissa AU - de Vries M AD - Department of Research, Arkin Mental Health Care, Klaprozenweg 111, 1033 NN, Amsterdam, the Netherlands. Electronic address: marissa.de.vries@arkin.nl. FAU - de Beurs, Edwin AU - de Beurs E AD - Department of Research, Arkin Mental Health Care, Klaprozenweg 111, 1033 NN, Amsterdam, the Netherlands; Department of Clinical Psychology, Faculty of Social Sciences, University of Leiden, Wassenaarseweg 52, 2333 AK, Leiden, the Netherlands. Electronic address: Edwin.de.Beurs@arkin.nl. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Subst Use Addict Treat JT - Journal of substance use and addiction treatment JID - 9918541186406676 SB - IM OTO - NOTNLM OT - Alcohol-use-disorder OT - Allocation OT - Cost-effectiveness OT - Detoxification OT - Inpatient OT - Outpatient OT - Predictors COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:35 PHST- 2026/01/31 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:35 [entrez] AID - S2949-8759(26)00264-X [pii] AID - 10.1016/j.josat.2026.210144 [doi] PST - aheadofprint SO - J Subst Use Addict Treat. 2026 Oct 27:210144. doi: 10.1016/j.josat.2026.210144. PMID- 42946170 OWN - NLM STAT- Publisher LR - 20261027 IS - 2667-0747 (Electronic) IS - 2667-0747 (Linking) DP - 2026 Oct 27 TI - Protein-Ligand Binding Database Tools for Selecting, Visualizing, and Comparing Interaction Thermodynamics Using Web Applications. PG - 100296 LID - S2667-0747(26)00049-2 [pii] LID - 10.1016/j.bpr.2026.100296 [doi] AB - Protein-small molecule binding thermodynamics and structural recognition is a significant challenge in molecular biophysics and difficult to model computationally. To apply AI-ML approaches for rational drug design of small molecules, a sufficiently large database is required that contains reliable and well-organized machine-readable experimental raw data. Current databases contain data on highly diverse proteins and small molecules, but even small chemical changes can cause large differences in binding affinities, so-called Activity Cliff. Therefore, we have assembled a database where the proteins, namely, carbonic anhydrases (CA), are similar and the compounds are primary sulfonamides that are structurally closely related. Despite high similarity of compound chemical structures, the affinities span from undetectable to femtomolar. The database currently contains data on 12 catalytically active human CA isozymes, approximately 800 compounds, and 10,000 interactions by four techniques: fluorescent thermal shift assay, isothermal titration calorimetry, surface plasmon resonance, and inhibition of enzymatic activity. The thermodynamic data are complemented with 138 X-ray crystal structures of the same ligands bound to 6 CA isozymes. Here we describe the functionalities of tools for web users of the Protein-Ligand Binding Database for the visualization and analysis of data, available through a collection of web applications presented at https://tools.plbd.org. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Inc. All rights reserved. FAU - Linge, Darius AU - Linge D AD - Department of Biothermodynamics and Drug Design, Institute of Biotechnology, Life Sciences Center, Vilnius University, Sauletekio 7, Vilnius, LT-10257, Lithuania. FAU - Matulis, Daumantas AU - Matulis D AD - Department of Biothermodynamics and Drug Design, Institute of Biotechnology, Life Sciences Center, Vilnius University, Sauletekio 7, Vilnius, LT-10257, Lithuania. Electronic address: daumantas.matulis@bti.vu.lt. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Biophys Rep (N Y) JT - Biophysical reports JID - 9918266001106676 SB - IM EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:35 PHST- 2026/07/03 00:00 [received] PHST- 2026/09/11 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:35 [entrez] AID - S2667-0747(26)00049-2 [pii] AID - 10.1016/j.bpr.2026.100296 [doi] PST - aheadofprint SO - Biophys Rep (N Y). 2026 Oct 27:100296. doi: 10.1016/j.bpr.2026.100296. PMID- 42946157 OWN - NLM STAT- Publisher LR - 20261027 IS - 1095-8541 (Electronic) IS - 0022-5193 (Linking) DP - 2026 Oct 27 TI - A neutral epidemic model of tick-borne pathogen transmission with co-infection and co-transmission. PG - 112629 LID - S0022-5193(26)00253-5 [pii] LID - 10.1016/j.jtbi.2026.112629 [doi] AB - With a single circulating tick-borne pathogen in a population of ticks and vertebrate hosts, the basic reproduction number incorporates contributions from tick-to-tick, tick-to-vertebrate host, and vertebrate host-to-tick transmission routes. With two co-circulating tick-borne pathogens, resident and invasive, and under the assumption that tick-to-tick is the only transmission route in a tick population feeding on vertebrate hosts, the invasion reproduction number is a measure of the ability of the invasive strain to take over the population at the resident-strain equilibrium. Predicting whether an invasive strain, that is identical to the resident one, will dominate depends on whether the mathematical model characterising the system possesses the neutrality property. However, even if a model is neutral and the strains not identical, its associated invasion reproduction number depends on model structure. We propose a two-slot model for tick-borne pathogen transmission in the presence of tick co-feeding, co-infection, and co-transmission, which is an extension of a previous co-infection and co-transmission model proposed by Alizon in 2013. Our two-slot model, with one population of susceptible (not infected) ticks, two populations of ticks infected with a single strain (resident or invasive), one population of co-infected (infected with both strains) ticks, and two populations doubly-infected with the same strain (resident or invasive), satisfies the neutrality property. We analyse the invasion reproduction number with the next-generation method and numerical simulations. We apply the two-slot model to compute the fraction of co-infected questing adult ticks at the end of a season as a function of the co-transmission probability, and discuss the potential risk posed to humans by such co-infected ticks. CI - Copyright (c) 2025. Published by Elsevier Ltd. FAU - Belluccini, Giulia AU - Belluccini G AD - Theoretical Biology and Biophysics Group, Los Alamos National Laboratory, Los Alamos, 87545, NM, USA; School of Mathematics, University of Leeds, Leeds, LS2 9JT, UK. FAU - Lin, Qianying AU - Lin Q AD - Theoretical Biology and Biophysics Group, Los Alamos National Laboratory, Los Alamos, 87545, NM, USA. FAU - Williams, Bevelynn AU - Williams B AD - School of Mathematics, University of Leeds, Leeds, LS2 9JT, UK. FAU - Lou, Yijun AU - Lou Y AD - Department of Applied Mathematics, Hong Kong Polytechnic University, Hong Kong SAR, China. FAU - Vatansever, Zati AU - Vatansever Z AD - Department of Parasitology, Faculty of Veterinary Medicine, Kafkas University, Kars, Turkey. FAU - Lopez-Garcia, Martin AU - Lopez-Garcia M AD - School of Mathematics, University of Leeds, Leeds, LS2 9JT, UK. FAU - Lythe, Grant AU - Lythe G AD - School of Mathematics, University of Leeds, Leeds, LS2 9JT, UK. FAU - Leitner, Thomas AU - Leitner T AD - Theoretical Biology and Biophysics Group, Los Alamos National Laboratory, Los Alamos, 87545, NM, USA. FAU - Romero-Severson, Ethan AU - Romero-Severson E AD - Theoretical Biology and Biophysics Group, Los Alamos National Laboratory, Los Alamos, 87545, NM, USA. FAU - Molina-Paris, Carmen AU - Molina-Paris C AD - Theoretical Biology and Biophysics Group, Los Alamos National Laboratory, Los Alamos, 87545, NM, USA; School of Mathematics, University of Leeds, Leeds, LS2 9JT, UK. Electronic address: molina-paris@lanl.gov. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - J Theor Biol JT - Journal of theoretical biology JID - 0376342 SB - IM OTO - NOTNLM OT - basic reproduction number OT - co-feeding OT - co-infection OT - co-transmission OT - invasion reproduction number OT - mathematical model OT - neutrality condition OT - tick-borne pathogen COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:35 PHST- 2024/04/23 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:35 [entrez] AID - S0022-5193(26)00253-5 [pii] AID - 10.1016/j.jtbi.2026.112629 [doi] PST - aheadofprint SO - J Theor Biol. 2026 Oct 27:112629. doi: 10.1016/j.jtbi.2026.112629. PMID- 42946155 OWN - NLM STAT- Publisher LR - 20261027 IS - 1878-5832 (Electronic) IS - 1359-6446 (Linking) DP - 2026 Oct 27 TI - Incretin and nutrient-sensing GPCR therapeutics in type 2 diabetes: what target-modality-formulation matching explains. PG - 104843 LID - S1359-6446(26)00248-5 [pii] LID - 10.1016/j.drudis.2026.104843 [doi] AB - Receptor-directed therapeutics now dominate type 2 diabetes, yet their clinical value depends on more than target biology. This review proposes a pharmaceutics-centered framework spanning the receptors for glucagon-like peptide-1, glucose-dependent insulinotropic polypeptide and glucagon, amylin co-agonism and selected nutrient-sensing receptors, it then tests against a defined cohort of 49 programs that reached Phase II in type 2 diabetes. Among arrests with a disclosed scientific cause, modality predominates; two-thirds of arrests were unexplained or attributed to portfolio decisions. Deliverability discriminates among comparable mechanisms, cannot raise a low receptor ceiling and is poorly served by the public record. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved. FAU - Seo, Yohan AU - Seo Y AD - Department of Physiology, Dongguk University College of Medicine, Gyeongju 38066, the Republic of Korea. Electronic address: ddukdae12@dongguk.ac.kr. FAU - Woo, Joohan AU - Woo J AD - Department of Physiology, Dongguk University College of Medicine, Gyeongju 38066, the Republic of Korea; Channelopathy Research Center, Dongguk University College of Medicine, 32 Dongguk-ro, Goyang 10326, the Republic of Korea. Electronic address: gabriel929@dongguk.ac.kr. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - England TA - Drug Discov Today JT - Drug discovery today JID - 9604391 SB - IM OTO - NOTNLM OT - G-protein-coupled receptor OT - GIP receptor OT - GLP-1 receptor OT - Type 2 diabetes OT - incretin OT - oral peptide delivery OT - orforglipron COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Specifically: both authors are salaried academic staff of Dongguk University College of Medicine, and Joohan Woo is additionally affiliated with the Channelopathy Research Center, Goyang, Republic of Korea. Neither author holds an appointment, consultancy, advisory role or honorary position with any pharmaceutical or biotechnology company whose products are discussed in this review, and neither has received consultancy fees, speaker honoraria, travel support, research funding, equity or payment in kind from any such company. Neither author holds a patent, patent application or licensing interest relating to any agent, target or delivery technology discussed here. Neither author holds an editorial position with Drug Discovery Today. The reference list contains no self-citation. A completed declaration-of-interest form is submitted as a separate file. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:35 PHST- 2026/07/28 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:35 [entrez] AID - S1359-6446(26)00248-5 [pii] AID - 10.1016/j.drudis.2026.104843 [doi] PST - aheadofprint SO - Drug Discov Today. 2026 Oct 27:104843. doi: 10.1016/j.drudis.2026.104843. PMID- 42946151 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-1597 (Electronic) IS - 1572-1000 (Linking) DP - 2026 Oct 27 TI - Patient-level visible-near-infrared hyperspectral histopathology for normal skin, actinic keratosis, and cutaneous squamous cell carcinoma. PG - 105696 LID - S1572-1000(26)00361-3 [pii] LID - 10.1016/j.pdpdt.2026.105696 [doi] AB - BACKGROUND: Actinic keratosis (AK) and cutaneous squamous cell carcinoma (cSCC) form a biologically and morphologically continuous keratinocytic spectrum in which borderline lesions can be difficult to classify reproducibly. Previous optical studies have often focused on in-vivo spectra, binary tasks, or patch-level evaluation, leaving the patient-level three-class histopathology problem insufficiently studied. We investigated whether visible-near-infrared hyperspectral imaging (VIS-NIR HSI) could provide complementary information to routine morphology as an adjunctive computational tool. METHODS: This retrospective study included 127 patients with histologically confirmed normal skin (n=43), AK (n=40), or cSCC (n=44). Patients were separated before patch extraction into training (n=82), validation (n=20), and locked independent test (n=25) cohorts. Unstained sections were imaged over 367.5-974.9 nm with 128 spectral bands, and adjacent hematoxylin-and-eosin sections guided region-of-interest selection. SkinHSI-MIL is a pathologist-guided spectral-spatial multiple-instance learning framework that combines multi-scale spectral encoding, learnable wavelength projection for spatial morphology, gated feature fusion, and gated-attention aggregation to generate one prediction per patient while preventing patch-level information leakage. RESULTS: In the independent test cohort, SkinHSI-MIL correctly classified 23 of 25 patients, yielding 92.0% accuracy (95% CI, 75.0-97.8%), 90.3% macro-sensitivity, 95.9% macro-specificity, and 90.8% macro-F1. The macro-AUC was 0.965 (95% CI, 0.917-0.995); class-specific AUCs were 0.984 (95% CI, 0.935-1.000) for normal skin, 0.944 (0.842-1.000) for AK, and 0.968 (0.900-1.000) for cSCC. Because the test set contained only 25 patients, including six AK cases, these estimates should be regarded as preliminary rather than definitive evidence of clinical diagnostic accuracy. CONCLUSIONS: Patient-level VIS-NIR hyperspectral histopathology with spectral-spatial MIL showed encouraging feasibility for three-class discrimination of normal skin, AK, and cSCC while preserving the patient as the statistical unit. The system remains pathologist-guided and is intended as an adjunct to histopathology, not a replacement. Multi-center external validation, acquisition standardization, and prospective workflow studies are required before clinical translation. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Xu, Congcong AU - Xu C AD - Department of Dermatology, Qilu Hospital of Shandong University, Jinan, Shandong, China. FAU - Meng, Feifei AU - Meng F AD - Shandong University of Traditional Chinese Medicine, Jinan 250355, Shandong, China. FAU - Si, Xiaoqing AU - Si X AD - Department of Dermatology, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, Jinan 250014, Shandong, China. Electronic address: 1771641128@qq.com. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Photodiagnosis Photodyn Ther JT - Photodiagnosis and photodynamic therapy JID - 101226123 SB - IM OTO - NOTNLM OT - actinic keratosis OT - cutaneous squamous cell carcinoma OT - digital pathology OT - hyperspectral imaging OT - multiple-instance learning OT - photodiagnosis COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:35 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:35 [entrez] AID - S1572-1000(26)00361-3 [pii] AID - 10.1016/j.pdpdt.2026.105696 [doi] PST - aheadofprint SO - Photodiagnosis Photodyn Ther. 2026 Oct 27:105696. doi: 10.1016/j.pdpdt.2026.105696. PMID- 42946149 OWN - NLM STAT- Publisher LR - 20261027 IS - 1096-1186 (Electronic) IS - 1043-6618 (Linking) DP - 2026 Oct 27 TI - Mitochondrial Transplantation as a Therapeutic Strategy: From Energy Replacement to Metabolic and Immune Reprogramming. PG - 109708 LID - S1043-6618(26)01623-3 [pii] LID - 10.1016/j.phrs.2026.109708 [doi] AB - Mitochondrial dysfunction drives diseases, including ischemia-reperfusion injury, neurodegenerative disorders, cardiovascular disease, metabolic syndrome, and inflammation. Conventional mitochondria-targeted strategies offer only partial rescue and are insufficient for severe, irreversible damage. Mitochondrial transplantation (MT)-the delivery of exogenously isolated functional mitochondria to injured cells-has emerged as a promising therapeutic strategy that directly restores mitochondrial bioenergetic function and may subsequently modify the tissue microenvironment. This Review examines the entire MT pipeline, from mitochondrial source selection, isolation and preservation, to in vivo delivery strategies. We critically examine the multiple mechanisms underlying MT's therapeutic efficacy, including cellular uptake and intracellular integration, energy restoration and metabolic reprogramming, redox rebalancing, remodeling of mitochondrial quality control (fusion/fission, mitophagy, biogenesis), and immunomodulation. We summarize preclinical and clinical applications across multiple organ systems-heart, nervous system, liver, kidney, lung, skeletal muscle, retina-as well as in oncology and aging. We analyze five major translational bottlenecks: the lack of standardized manufacturing protocols, undefined dose-response relationships, immunogenicity and long-term safety uncertainties, the decisive impact of mitochondrial heterogeneity, and regulatory ambiguity. We outline future directions-mechanistic deepening, engineered target delivery, individualized mitochondrial medicine, combinatorial regimens, and a phased clinical roadmap. MT is not merely energy supplementation; it may reshape cellular metabolism, quality control networks, and immune microenvironments, representing a shift from pharmacological modulation of mitochondrial function toward organelle replacement strategies. Despite challenges, these advances may facilitate individualized mitochondrial therapies as a proposed future direction. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved. FAU - Zhu, Chuli AU - Zhu C AD - Jiangsu Key Laboratory of Tissue Engineering and Neuroregeneration, Key Laboratory of Neuroregeneration of Ministry of Education, Co-innovation Center of Neuroregeneration, Medical School of Nantong University, Nantong University, Nantong, Jiangsu Province 226001, PR China. FAU - Xu, Meng AU - Xu M AD - Jiangsu Key Laboratory of Tissue Engineering and Neuroregeneration, Key Laboratory of Neuroregeneration of Ministry of Education, Co-innovation Center of Neuroregeneration, Medical School of Nantong University, Nantong University, Nantong, Jiangsu Province 226001, PR China. FAU - Ji, Yanan AU - Ji Y AD - Jiangsu Key Laboratory of Tissue Engineering and Neuroregeneration, Key Laboratory of Neuroregeneration of Ministry of Education, Co-innovation Center of Neuroregeneration, Medical School of Nantong University, Nantong University, Nantong, Jiangsu Province 226001, PR China. FAU - Wang, Jie AU - Wang J AD - Jiangsu Key Laboratory of Tissue Engineering and Neuroregeneration, Key Laboratory of Neuroregeneration of Ministry of Education, Co-innovation Center of Neuroregeneration, Medical School of Nantong University, Nantong University, Nantong, Jiangsu Province 226001, PR China. FAU - Yao, Xinlei AU - Yao X AD - Jiangsu Key Laboratory of Tissue Engineering and Neuroregeneration, Key Laboratory of Neuroregeneration of Ministry of Education, Co-innovation Center of Neuroregeneration, Medical School of Nantong University, Nantong University, Nantong, Jiangsu Province 226001, PR China. Electronic address: xinlei_yao@ntu.edu.cn. FAU - Sun, Hualin AU - Sun H AD - Jiangsu Key Laboratory of Tissue Engineering and Neuroregeneration, Key Laboratory of Neuroregeneration of Ministry of Education, Co-innovation Center of Neuroregeneration, Medical School of Nantong University, Nantong University, Nantong, Jiangsu Province 226001, PR China. Electronic address: sunhl@ntu.edu.cn. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Netherlands TA - Pharmacol Res JT - Pharmacological research JID - 8907422 SB - IM OTO - NOTNLM OT - clinical translation OT - individualized mitochondrial medicine OT - metabolic reprogramming OT - mitochondrial dysfunction OT - mitochondrial quality control OT - mitochondrial transplantation COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Competing interests No competing interests. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:35 PHST- 2026/08/25 00:00 [received] PHST- 2026/10/25 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:35 [entrez] AID - S1043-6618(26)01623-3 [pii] AID - 10.1016/j.phrs.2026.109708 [doi] PST - aheadofprint SO - Pharmacol Res. 2026 Oct 27:109708. doi: 10.1016/j.phrs.2026.109708. PMID- 42946148 OWN - NLM STAT- Publisher LR - 20261027 IS - 1095-953X (Electronic) IS - 0969-9961 (Linking) DP - 2026 Oct 27 TI - Enhanced auditory gain and tinnitus-like behavior in mice lacking KCTD12, an auxiliary subunit of GABA(B) receptors. PG - 107664 LID - S0969-9961(26)00410-9 [pii] LID - 10.1016/j.nbd.2026.107664 [doi] AB - Precise regulation of neural gain is essential for accurate sensory processing and relies on inhibitory mechanisms that constrain neuronal responsiveness. Disruption of inhibitory control has been implicated in disorders characterized by abnormal sensory perception, including tinnitus, yet the molecular mechanisms regulating inhibitory signaling remain poorly understood. Here, we investigated the role of potassium channel tetramerization domain-containing protein 12 (KCTD12)-an auxiliary subunit of the gamma-aminobutyric acid type B receptor (GABA(B)R) and a proposed risk modifier for chronic tinnitus-in auditory circuit function using KCTD12-deficient mice. Despite abundant KCTD12 expression in the cochlea, deletion of Kctd12 did not affect endocochlear potential, outer hair cell function, or auditory thresholds, demonstrating preserved peripheral auditory sensitivity. In contrast, KCTD12-deficient mice displayed enhanced suprathreshold auditory responses, increased acoustic startle reactivity, and deficits in gap-prepulse inhibition of acoustic startle, consistent with altered auditory gain and tinnitus-like behavior. Electrophysiological recordings from the inferior colliculus revealed increased spontaneous activity, enhanced sound-evoked responses, and steeper rate-level functions, indicating elevated central auditory responsiveness. Within the dorsal cochlear nucleus, KCTD12 was selectively expressed in inhibitory cartwheel cells, where its loss reduced GABA(B)R expression and baclofen-evoked responses, while excitatory fusiform neurons were largely unaffected. These findings identify KCTD12 as a cell-type-specific modulator of GABA(B)R-mediated inhibition and demonstrate that its loss increases neural gain in central auditory circuits and produces tinnitus-like behavior. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Melichar, Adolf AU - Melichar A AD - Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Krc, Czech Republic; Second Faculty of Medicine, Charles University, 150 06 Prague, Czech Republic. FAU - Suchankova, Stepanka AU - Suchankova S AD - Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Krc, Czech Republic. FAU - Rybalko, Natalia AU - Rybalko N AD - Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Krc, Czech Republic. FAU - Pysanenko, Kateryna AU - Pysanenko K AD - Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Krc, Czech Republic. FAU - Hruskova, Bohdana AU - Hruskova B AD - Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Krc, Czech Republic. FAU - Suta, Daniel AU - Suta D AD - Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Krc, Czech Republic. FAU - Dittrichova, Jana AU - Dittrichova J AD - Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Krc, Czech Republic. FAU - Jovanovic, Natasa AU - Jovanovic N AD - Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Krc, Czech Republic; Second Faculty of Medicine, Charles University, 150 06 Prague, Czech Republic. FAU - Kozelkova, Klara AU - Kozelkova K AD - Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Krc, Czech Republic. FAU - Bettler, Bernhard AU - Bettler B AD - Department of Biomedicine, Pharmazentrum, University of Basel, Klingelbergstrasse 50, 4056 Basel, Switzerland. FAU - Pavlinkova, Gabriela AU - Pavlinkova G AD - Institute of Biotechnology, Academy of Sciences of the Czech Republic, Prumyslova 595, 25250 Vestec, Czech Republic. FAU - Turecek, Rostislav AU - Turecek R AD - Department of Auditory Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Krc, Czech Republic. Electronic address: rostislav.turecek@iem.cas.cz. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Neurobiol Dis JT - Neurobiology of disease JID - 9500169 SB - IM OTO - NOTNLM OT - Auditory system OT - GABA(B)R OT - KCTD12 OT - Tinnitus COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:35 PHST- 2026/08/14 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:35 [entrez] AID - S0969-9961(26)00410-9 [pii] AID - 10.1016/j.nbd.2026.107664 [doi] PST - aheadofprint SO - Neurobiol Dis. 2026 Oct 27:107664. doi: 10.1016/j.nbd.2026.107664. PMID- 42946144 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-2968 (Electronic) IS - 0006-2952 (Linking) VI - 254 IP - Pt 4 DP - 2026 Oct 27 TI - Mechanisms of cetuximab resistance in head and neck squamous cell Carcinoma: Focus on the EGFR-ERK pathway. PG - 118568 LID - S0006-2952(26)00911-1 [pii] LID - 10.1016/j.bcp.2026.118568 [doi] AB - Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer worldwide. Major risk factors include tobacco use, excessive alcohol consumption, and infection with oncogenic human papillomaviruses (HPV). Despite advances in treatment, HNSCC remains associated with poor outcomes, particularly in patients with advanced or recurrent/metastatic disease. The epidermal growth factor receptor (EGFR) has been recognized as a key oncogenic driver in HNSCC, as it is frequently overexpressed and promotes activation of multiple pro-tumorigenic signaling pathways, including ERK and PI3K/Akt. Cetuximab remains the only approved molecularly targeted agent directed against a tumor-associated receptor in HNSCC. However, its clinical benefit is limited by low response rates and the frequent development of resistance even in initially responsive tumors. Understanding the molecular basis of resistance to EGFR-targeted therapy is therefore critical for developing more effective pharmacological strategies and improving the clinical utility of cetuximab. In this review, we summarize EGFR alterations, activation of parallel compensatory pathways, and the reactivation of downstream signaling cascades, including PI3K/Akt and Ras/ERK, as major mechanisms underlying resistance to EGFR-targeted therapy in HNSCC. We focus particularly on the ERK signaling pathway as a central integrator of genetic, epigenetic, and adaptive resistance mechanisms. Sustained ERK activity restores downstream EGFR outputs, promotes feedback ligand production, drives epithelial-mesenchymal transition and cellular plasticity, and engages parallel oncogenic pathways, thereby enabling tumor cells to evade cetuximab-mediated EGFR blockade. Combined targeting of ERK and EGFR may therefore represent a promising strategy for overcoming cetuximab resistance in selected molecular contexts. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Galiyeva, Liliya AU - Galiyeva L AD - Department of Otorhinolaryngology, Head and Neck Surgery, First Medical Faculty, Charles University, University Hospital Motol and Homolka, V Uvalu 84, Prague CZ-15006, Czech Republic; Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, Prague, Czech Republic. FAU - Grusanovic, Josipa AU - Grusanovic J AD - Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, Prague, Czech Republic; Department of Cell Biology, Faculty of Science, Charles University, Prague, Czech Republic. FAU - Netusil, Jiri AU - Netusil J AD - Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, Prague, Czech Republic. FAU - Melichar, Vojtech AU - Melichar V AD - Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, Prague, Czech Republic. FAU - Klimova, Zuzana AU - Klimova Z AD - Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, Prague, Czech Republic. FAU - Kolar, Michal AU - Kolar M AD - Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, Prague, Czech Republic. Electronic address: kolarmi@img.cas.cz. FAU - Plzak, Jan AU - Plzak J AD - Department of Otorhinolaryngology, Head and Neck Surgery, First Medical Faculty, Charles University, University Hospital Motol and Homolka, V Uvalu 84, Prague CZ-15006, Czech Republic. FAU - Sachova, Jana AU - Sachova J AD - Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Videnska 1083, Prague, Czech Republic. FAU - Kalfert, David AU - Kalfert D AD - Department of Otorhinolaryngology, Head and Neck Surgery, First Medical Faculty, Charles University, University Hospital Motol and Homolka, V Uvalu 84, Prague CZ-15006, Czech Republic. Electronic address: david.kalfert@fnmotol.cz. FAU - Vomastek, Tomas AU - Vomastek T AD - Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, Prague, Czech Republic. Electronic address: vomy@biomed.cas.cz. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - England TA - Biochem Pharmacol JT - Biochemical pharmacology JID - 0101032 SB - IM OTO - NOTNLM OT - Cetuximab OT - EGFR OT - ERK OT - HNSCC OT - HPV OT - Ras COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:35 PHST- 2026/07/22 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 20:35 [entrez] AID - S0006-2952(26)00911-1 [pii] AID - 10.1016/j.bcp.2026.118568 [doi] PST - aheadofprint SO - Biochem Pharmacol. 2026 Oct 27;254(Pt 4):118568. doi: 10.1016/j.bcp.2026.118568. PMID- 42946142 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-1732 (Electronic) IS - 0079-6107 (Linking) DP - 2026 Oct 27 TI - Lysosomal biophysics in CLN3 disease: Connecting membrane transport, molecular homeostasis, and tissue dysfunction. PG - 101964 LID - S0079-6107(26)00081-7 [pii] LID - 10.1016/j.pbiomolbio.2026.101964 [doi] AB - CLN3 disease (CLN3 Batten disease) is a lysosomal neurodegenerative disorder characterized by early retinal degeneration and progressive neurological dysfunction, yet the mechanisms connecting CLN3-dependent membrane physiology to selective cellular and tissue vulnerability remain unresolved. This review integrates current evidence across four experimentally separable scales: membrane biophysics, molecular and organellar homeostasis, cell-specific function, and tissue pathology. Direct electrophysiological studies support a luminal pH-sensitive CLN3-dependent lysosomal chloride conductance associated, in tested systems, with changes in luminal chloride, pH, cathepsin activity, and protein degradation. Independently, CLN3 deficiency disrupts glycerophosphodiester clearance, phospholipid and cholesterol handling, cation-independent mannose 6-phosphate receptor trafficking, hydrolase delivery, and autophagic lysosomal reformation. These organellar abnormalities coexist with distinct phenotypes in retinal pigment epithelium, neurons, microglia, astrocytes, and brain endothelial cells, together with retinal, neural-circuit, and myelin abnormalities at the tissue level. Current evidence does not establish a universal chloride-first or storage-first cascade; instead, it supports interacting CLN3-dependent modules whose causal ordering may vary by cell type, model, and disease stage. We therefore propose a multiscale mechanistic framework that links measurable lysosomal physical variables to molecular homeostasis, cell-specific function, and tissue vulnerability while preserving the distinction between direct evidence and mechanistic inference. This framework defines testable priorities for causal ordering, rescue experiments, and mechanism-matched target engagement in CLN3 disease. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Al Abdulsalam, Najla Khaled AU - Al Abdulsalam NK AD - Biological Sciences Department, College of Science, King Faisal University, Al Ahsa 31982, Saudi Arabia. Electronic address: najlaks@kfu.edu.sa. FAU - Hamad, Rabab S AU - Hamad RS AD - Biological Sciences Department, College of Science, King Faisal University, Al Ahsa 31982, Saudi Arabia. Electronic address: rhamad@kfu.edu.sa. FAU - Elmorsy, Elsayed A AU - Elmorsy EA AD - Department of Pharmacology and Toxicology, College of Pharmacy, Qassim University, Buraidah 52571, Saudi Arabia; Department of Clinical Pharmacology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt. Electronic address: a.almarsa@qu.edu.sa. FAU - Saber, Sameh AU - Saber S AD - Department of Pharmacology, Faculty of Pharmacy, Delta University for Science and Technology, Gamasa 11152, Egypt. Electronic address: sameh.saber@deltauniv.edu.eg. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - England TA - Prog Biophys Mol Biol JT - Progress in biophysics and molecular biology JID - 0401233 SB - IM OTO - NOTNLM OT - CLN3 disease OT - Endolysosomal homeostasis OT - Glycerophosphodiester metabolism OT - Lysosomal ion transport OT - Membrane biophysics OT - Tissue vulnerability COIS- Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:35 PHST- 2026/08/17 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:35 [entrez] AID - S0079-6107(26)00081-7 [pii] AID - 10.1016/j.pbiomolbio.2026.101964 [doi] PST - aheadofprint SO - Prog Biophys Mol Biol. 2026 Oct 27:101964. doi: 10.1016/j.pbiomolbio.2026.101964. PMID- 42946126 OWN - NLM STAT- Publisher LR - 20261027 IS - 1879-8519 (Electronic) IS - 1879-8500 (Linking) DP - 2026 Oct 27 TI - Hypofractionated Radiation Therapy for Localized Prostate Cancer: An ASTRO Clinical Practice Guideline. LID - S1879-8500(26)00326-7 [pii] LID - 10.1016/j.prro.2026.09.017 [doi] AB - PURPOSE: This guideline provides evidence-based recommendations on the use of hypofractionated external beam radiation therapy (EBRT) in patients with localized prostate cancer, including indications for moderate hypofractionation (MHF) and ultrahypofractionation (UHF), appropriate dose-fractionation regimens, target volumes, and treatment planning and delivery techniques. METHODS: The American Society for Radiation Oncology convened a multidisciplinary task force to address 5 key questions related to hypofractionated EBRT in localized prostate cancer. Recommendations were based on a systematic literature review and developed using a predefined consensus-based methodology with grading of quality of evidence and strength of recommendation. RESULTS: MHF is recommended over conventional fractionation for patients receiving EBRT across all risk groups, supported by high-quality randomized data demonstrating equivalent oncologic outcomes, toxicity, and quality of life, with shorter treatment duration that may reduce overall treatment burden. UHF is recommended as a standard treatment option for patients with low- and intermediate-risk disease electing for definitive EBRT and may be considered in selected patients with high-risk disease, preferably in the context of clinical trials. Selection of fractionation should generally be independent of patient age, comorbidity, and use of androgen deprivation therapy, although MHF may be preferred over UHF for patients with significant baseline urinary dysfunction. Evidence supports the use of contemporary image-guided, conformal treatment techniques to optimize target coverage and minimize toxicity. Guidance is also provided regarding appropriate dose-fractionation regimens, including commonly used MHF and UHF schedules, as well as target volumes and integration of pelvic nodal irradiation. CONCLUSIONS: Hypofractionated EBRT has become the predominant form of definitive radiation therapy for localized prostate cancer, supported by mature randomized evidence demonstrating comparable outcomes to conventional fractionation and reduced treatment burden. Ongoing studies will further refine patient selection, the role of UHF in higher-risk disease, and integration of advanced imaging and adaptive technologies. These evidence-based recommendations inform best clinical practices in the use of hypofractionated EBRT for localized prostate cancer. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Morgan, Scott C AU - Morgan SC AD - Department of Radiation Oncology, The Ottawa Hospital and University of Ottawa, Ottawa, Ontario, Canada. Electronic address: smorgan@toh.ca. FAU - Halthore, Aditya AU - Halthore A AD - Department of Radiation Oncology, Johns Hopkins University, Baltimore, Maryland. FAU - Jia, Angela Y AU - Jia AY AD - Department of Radiation Oncology, University Hospitals Cleveland Medical Center, Cleveland, Ohio. FAU - Kishan, Amar U AU - Kishan AU AD - Department of Radiation Oncology, University of California, Los Angeles, Los Angeles, California. FAU - Loblaw, Andrew AU - Loblaw A AD - Department of Radiation Oncology, Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada. FAU - Shen, Xinglei AU - Shen X AD - Department of Radiation Oncology, University of Kansas Medical Center, Kansas City, Kansas. FAU - Tree, Alison AU - Tree A AD - Department of Radiation Oncology, The Royal Marsden NHS Foundation Trust and The Institute of Cancer Research, Sutton, Surrey, United Kingdom. FAU - DeMarco, John AU - DeMarco J AD - Department of Radiation Oncology, Cedars-Sinai Medical Center, Los Angeles, California. FAU - Dess, Robert T AU - Dess RT AD - Department of Radiation Oncology, University of Michigan, Ann Arbor, Michigan. FAU - Eastwick, Gary AU - Eastwick G AD - Department of Radiation Oncology, MD Anderson Cancer Center at Cooper, Camden, New Jersey. FAU - Ellis, Rodney AU - Ellis R AD - Department of Radiation Oncology, USF Health Morsani College of Medicine, Tampa, Florida. FAU - Feldman, Adam S AU - Feldman AS AD - Department of Urology, Mass General Brigham, Boston, Massachusetts. FAU - Kelly, Wm Kevin AU - Kelly WK AD - Department of Medical Oncology, Thomas Jefferson University, Philadelphia, Pennsylvania. FAU - Morrow, Eric AU - Morrow E AD - Patient Representative, Bowie, Maryland. FAU - Ost, Piet AU - Ost P AD - Department of Radiation Oncology, Iridium Network, Wilrijk, Vlaanderen, Belgium. FAU - Wang, Edina C AU - Wang EC AD - Department of Radiation Oncology, Mercy Hospital St. Louis, St. Louis, Missouri. FAU - Washington, Samuel L 3rd AU - Washington SL 3rd AD - Department of Urology, University of California, San Francisco, San Francisco, California. FAU - Weg, Emily S AU - Weg ES AD - Department of Radiation Oncology, Weill Cornell Medicine, New York, New York. FAU - Bradfield, Lisa AU - Bradfield L AD - American Society for Radiation Oncology, Arlington, Virginia. FAU - Hoffman, Karen AU - Hoffman K AD - Department of Radiation Oncology, University of Texas MD Anderson Cancer Center, Houston, Texas. LA - eng PT - Journal Article PT - Practice Guideline DEP - 20261027 PL - United States TA - Pract Radiat Oncol JT - Practical radiation oncology JID - 101558279 SB - IM EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:34 PHST- 2026/02/27 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:34 [entrez] AID - S1879-8500(26)00326-7 [pii] AID - 10.1016/j.prro.2026.09.017 [doi] PST - aheadofprint SO - Pract Radiat Oncol. 2026 Oct 27:S1879-8500(26)00326-7. doi: 10.1016/j.prro.2026.09.017. PMID- 42946122 OWN - NLM STAT- Publisher LR - 20261027 IS - 1535-7732 (Electronic) IS - 1051-0443 (Linking) DP - 2026 Oct 27 TI - Comprehensive Evaluation of Hemodialysis Arteriovenous Fistula Dysfunction Using 2D-Ultrasound, Elastography, and Vector Flow Imaging. PG - 109163 LID - S1051-0443(26)01174-7 [pii] LID - 10.1016/j.jvir.2026.109163 [doi] AB - OBJECTIVE: To evaluate the diagnostic value of multimodal ultrasound-including 2D-ultrasound, sound touch elastography (STE), and vector flow imaging (VFI)-for the identification of functional and biomechanical abnormalities indicative of arteriovenous fistula (AVF) stenosis or occlusion in hemodialysis patients. METHODS: This prospective study enrolled 37 hemodialysis patients with AVFs, divided into normal (n = 20) and dysfunction (n = 17) groups based on digital subtraction angiography (DSA) or clinical/ultrasound criteria. For the dysfunction group, AVF stenosis was defined as >50% luminal diameter reduction on DSA, and occlusion as complete absence of flow. For the normal group, dysfunction was excluded by clinical examination (thrill/bruit) and normal duplex parameters. All patients underwent 2D-ultrasound, STE, and VFI examinations. Key parameters measured included vessel diameter, elasticity (kPa), blood flow velocities, turbulence index, and wall shear stress (WSS). Statistical comparisons were made between groups. RESULTS: No significant differences were found in vessel diameter or intima status between groups. However, the stenosis/occlusion group exhibited significantly lower mean blood flow, peak systolic/end-diastolic velocities, and WSS, along with higher resistance index and vessel wall stiffness (STE-mean: 30.35 +/- 11.65 vs. 20.7 +/- 7.52 kPa, p < 0.01). VFI revealed reduced blood flow velocity, turbulence, and WSS in dysfunctional AVFs (all p < 0.01). CONCLUSION: Multimodal ultrasound integrating 2D-ultrasound, STE, and VFI provides a comprehensive assessment of AVF structure and function, detecting functional and biomechanical abnormalities associated with AVF stenosis or occlusion. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Chen, Nanjie AU - Chen N AD - Department of Ultrasound, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen 518020, Guangdong, China. Electronic address: nanjiechen@126.com. FAU - Guo, Baochun AU - Guo B AD - Department of Nephrology, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen 518020, Guangdong, China. Electronic address: guobaochun_1983@163.com. FAU - Wang, Zhen AU - Wang Z AD - Department of Nephrology, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen 518020, Guangdong, China. Electronic address: WZ1989JNU@163.com. FAU - Huang, Shaoting AU - Huang S AD - Department of Ultrasound, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen 518020, Guangdong, China. Electronic address: shouting12345@163.com. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Vasc Interv Radiol JT - Journal of vascular and interventional radiology : JVIR JID - 9203369 SB - IM OTO - NOTNLM OT - Arteriovenous fistula OT - Hemodialysis OT - Multimodal ultrasound OT - Sound touch elastography OT - Vector flow imaging EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:34 PHST- 2026/05/11 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:34 [entrez] AID - S1051-0443(26)01174-7 [pii] AID - 10.1016/j.jvir.2026.109163 [doi] PST - aheadofprint SO - J Vasc Interv Radiol. 2026 Oct 27:109163. doi: 10.1016/j.jvir.2026.109163. PMID- 42946120 OWN - NLM STAT- Publisher LR - 20261027 IS - 1553-4669 (Electronic) IS - 1553-4650 (Linking) DP - 2026 Oct 27 TI - Regarding "Minimally Invasive Splenectomy for Recurrent Gynecologic Cancer and Long-Term Outcomes". LID - S1553-4650(26)01006-X [pii] LID - 10.1016/j.jmig.2026.09.478 [doi] FAU - Hong, Yinwen AU - Hong Y AD - Department of Obstetrics and Gynecology, Suzhou Hospital of Integrated Traditional Chinese and Western Medicine, Suzhou, China. Electronic address: yinwen.hong1986@qq.com. LA - eng PT - Letter DEP - 20261027 PL - United States TA - J Minim Invasive Gynecol JT - Journal of minimally invasive gynecology JID - 101235322 SB - IM EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:34 PHST- 2026/09/04 00:00 [received] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:34 [entrez] AID - S1553-4650(26)01006-X [pii] AID - 10.1016/j.jmig.2026.09.478 [doi] PST - aheadofprint SO - J Minim Invasive Gynecol. 2026 Oct 27:S1553-4650(26)01006-X. doi: 10.1016/j.jmig.2026.09.478. PMID- 42946117 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-7544 (Electronic) IS - 0306-4522 (Linking) DP - 2026 Oct 27 TI - Intranasal insulin improves long-term cognitive deficits and protects hippocampal damage following neonatal hypoxia-ischemia in juvenile rats. LID - S0306-4522(26)00715-3 [pii] LID - 10.1016/j.neuroscience.2026.10.039 [doi] AB - Hypoxic-ischemic encephalopathy remains a significant cause of neonatal morbidity and mortality in neonates. Our study tested whether intranasal insulin provides long-term neuroprotection against neonatal hypoxia-ischemia (HI)-induced hippocampal damage and cognitive disturbances in juvenile rats. Postnatal day 10 (P10) Sprague-Dawley rats underwent right common carotid artery ligation or sham surgery followed by 90 min of hypoxia (8% oxygen) or room air, respectively. Immediately after, insulin (25 mug) or vehicle was administered in each naris, followed by repeated doses 24 and 48 h post-insult. HI caused acute cerebral ATP depletion, an acute inflammatory response characterized by increased IL-1beta, and corticosterone elevation. Intranasal insulin significantly attenuated ATP loss, reduced interleukin-1beta and corticosterone levels, and improved somatic growth. Behaviorally, intranasal insulin protected against neonatal HI-induced sensorimotor deficits, working memory impairments, and learning deficits in both sexes of juvenile rats (P21-P25). Male pups exhibited selective long-term memory deficits following HI, which were fully rescued by intranasal insulin. Histologically, neonatal HI induced hippocampal neuronal loss and increased Fluoro-Jade C + degenerating cells, together with loss of mature NeuN + neurons in hippocampal CA3, and suppression of dentate gyrus neurogenesis, with greater loss of immature DCX + neurons in P25 male rats. HI insult also resulted in brain inflammation, as indicated by microglia activation (Iba1 + ) and serum interleukin-1beta elevation. Intranasal insulin significantly reduced HI-induced brain inflammation and protected against hippocampal neuronal injury in both sexes. These findings demonstrate long-lasting neuroprotective effects of intranasal insulin following neonatal HI and support further investigation of this non-invasive approach for hypoxic-ischemic encephalopathy in neonates. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Dankhara, Nilesh AU - Dankhara N AD - Department of Pediatrics, Division of Newborn Medicine, University of Mississippi Medical Center, Jackson, MS 39216, USA. FAU - Lee, Jonathan W AU - Lee JW AD - Department of Pediatrics, Division of Newborn Medicine, University of Mississippi Medical Center, Jackson, MS 39216, USA. FAU - Ojeda, Norma B AU - Ojeda NB AD - Department of Advanced Biomedical Education, University of Mississippi Medical Center, Jackson, MS 39216, USA. FAU - Tucci, Michelle A AU - Tucci MA AD - Department of Anesthesiology, University of Mississippi Medical Center, Jackson, MS 39216, USA. FAU - Lu, Silu AU - Lu S AD - Department of Pediatrics, Division of Newborn Medicine, University of Mississippi Medical Center, Jackson, MS 39216, USA. FAU - White, Elizabeth L AU - White EL AD - Department of Pediatrics, Division of Newborn Medicine, University of Mississippi Medical Center, Jackson, MS 39216, USA. FAU - Winborne, Lindsey E AU - Winborne LE AD - Department of Pediatrics, Division of Newborn Medicine, University of Mississippi Medical Center, Jackson, MS 39216, USA. FAU - Xu, Sharon AU - Xu S AD - Department of Pediatrics, Division of Newborn Medicine, University of Mississippi Medical Center, Jackson, MS 39216, USA. FAU - Glendye, Carolyn K AU - Glendye CK AD - Department of Pediatrics, Division of Newborn Medicine, University of Mississippi Medical Center, Jackson, MS 39216, USA. FAU - Tien, Lu-Tai AU - Tien LT AD - School of Medicine, Fu Jen Catholic University, Xinzhuang Dist, New Taipei City 24205, Taiwan. FAU - Pang, Yi AU - Pang Y AD - Department of Pediatrics, Division of Newborn Medicine, University of Mississippi Medical Center, Jackson, MS 39216, USA. FAU - Bhatt, Abhay J AU - Bhatt AJ AD - Department of Pediatrics, Division of Newborn Medicine, University of Mississippi Medical Center, Jackson, MS 39216, USA. Electronic address: abhatt@umc.edu. FAU - Fan, Lir-Wan AU - Fan LW AD - Department of Pediatrics, Division of Newborn Medicine, University of Mississippi Medical Center, Jackson, MS 39216, USA. Electronic address: lwfan@umc.edu. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Neuroscience JT - Neuroscience JID - 7605074 SB - IM OTO - NOTNLM OT - Hippocampal damage OT - Inflammation OT - Intranasal insulin OT - Long-term cognitive disturbances OT - Neonatal hypoxia-ischemia OT - Neuroprotection COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships, which may be considered as potential competing interests: Lir-Wan Fan and Abhay Bhatt have a patent #US 11,931,403 B2, "Compositions, Systems, and Methods for Treating or Reducing Hypoxia-Ischemia Induced Brain Damage and Neurobehavioral Dysfunction in Neonates." to Lir-Wan Fan, Abhay Bhatt, and a patent application PCT/US2025/017087 (WO 2025/179292 A1), "Compositions, systems, and methods for treating or reducing hypoxia-ischemia induced brain damage and neurobehavioral dysfunction in neonates." to Lir-Wan Fan, Abhay Bhatt. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:34 PHST- 2026/05/05 00:00 [received] PHST- 2026/10/21 00:00 [revised] PHST- 2026/10/24 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:34 [entrez] AID - S0306-4522(26)00715-3 [pii] AID - 10.1016/j.neuroscience.2026.10.039 [doi] PST - aheadofprint SO - Neuroscience. 2026 Oct 27:S0306-4522(26)00715-3. doi: 10.1016/j.neuroscience.2026.10.039. PMID- 42946110 OWN - NLM STAT- Publisher LR - 20261027 IS - 1878-1519 (Electronic) IS - 1569-9048 (Linking) DP - 2026 Oct 27 TI - Point-of-Care Ultrasound During Diaphragmatic Isometric Contraction for Estimating Inspiratory Muscle Strength in Healthy Individuals. PG - 104669 LID - S1569-9048(26)00128-X [pii] LID - 10.1016/j.resp.2026.104669 [doi] AB - BACKGROUND: Diaphragm ultrasound (DUS) provides a non-invasive assessment of diaphragm structure and function. However, the physiological information captured may depend on both the parameter measured and the contractile condition during acquisition. Whether diaphragm thickness during maximal isometric inspiratory effort more closely reflects inspiratory pressure-generating capacity than conventional DUS indices remains unclear. OBJECTIVE: To investigate the association between maximal inspiratory pressure (MIP) and diaphragm thickness during maximal isometric inspiratory effort in healthy adults and compare it with conventional DUS parameters. METHODS: Seventy-six healthy volunteers (>/=18 years; 40 males) were enrolled. Diaphragmatic excursion (DE), diaphragm thickness at rest, during maximal inspiration and maximal isometric inspiratory effort, and thickening fraction (TF) were assessed by DUS. The highest of three measurements was analyzed. Correlation and regression analyses adjusted for age, sex, and height were performed. RESULTS: Median age was 43 years and BMI 22.8kg/m(2). MIP correlated with TF (r=0.39, p=0.035), DE during deep inspiration (r=0.46, p=0.011), and most strongly with diaphragm thickness during maximal isometric inspiratory effort (r=0.95, p<0.0001). Resting diaphragm thickness correlated inversely with MIP (r=-0.39, p=0.034). In multivariable analysis, thickness during maximal isometric inspiratory effort remained independently associated with MIP. CONCLUSIONS: In healthy adults, diaphragm thickness during maximal isometric inspiratory effort showed the strongest association with MIP among DUS parameters. These proof-of-concept findings support further investigation of this measurement as an ultrasound-based marker of inspiratory muscle strength, including setting in which conventional MIP assessment may not be feasible. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Scarlata, Simone AU - Scarlata S AD - Operative Research Unit of Internal Medicine, Universita Campus Bio-Medico, Roma, Italy; Unit of Internal Medicine, Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy, Via Alvaro del Portillo, 200, Roma, 00128, Italy. Electronic address: s.scarlata@policlinicocampus.it. FAU - Laudisio, Alice AU - Laudisio A AD - Research Unit of Geriatrics, Department of Medicine and Surgery, Universita Campus Bio-Medico di Roma, Via Alvaro del Portillo, 21, Roma, 00128, Italy. FAU - Salvati, Martina AU - Salvati M AD - Operative Research Unit of Internal Medicine, Universita Campus Bio-Medico, Roma, Italy; Unit of Internal Medicine, Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy, Via Alvaro del Portillo, 200, Roma, 00128, Italy. FAU - Cito, Cristina AU - Cito C AD - Unit of Internal Medicine, Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy, Via Alvaro del Portillo, 200, Roma, 00128, Italy. FAU - Perna, Marco AU - Perna M AD - Unit of Internal Medicine, Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy, Via Alvaro del Portillo, 200, Roma, 00128, Italy. FAU - Sergi, Gabriele AU - Sergi G AD - Operative Research Unit of Internal Medicine, Universita Campus Bio-Medico, Roma, Italy; Unit of Internal Medicine, Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy, Via Alvaro del Portillo, 200, Roma, 00128, Italy. FAU - Di Nunzio, Francesca AU - Di Nunzio F AD - Operative Research Unit of Internal Medicine, Universita Campus Bio-Medico, Roma, Italy. FAU - Lemme, Daniela AU - Lemme D AD - Operative Research Unit of Internal Medicine, Universita Campus Bio-Medico, Roma, Italy. FAU - Incalzi, Raffaele Antonelli AU - Incalzi RA AD - Operative Research Unit of Internal Medicine, Universita Campus Bio-Medico, Roma, Italy; Unit of Internal Medicine, Fondazione Policlinico Universitario Campus Bio-Medico, Rome, Italy, Via Alvaro del Portillo, 200, Roma, 00128, Italy. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Respir Physiol Neurobiol JT - Respiratory physiology & neurobiology JID - 101140022 SB - IM OTO - NOTNLM OT - diaphragm ultrasound OT - diaphragmatic excursion OT - inspiratory muscle strength OT - isometric diaphragm thickness OT - maximal inspiratory pressure OT - thickening fraction COIS- Declaration of Competing Interest The authors declare no conflicts of interest. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:34 PHST- 2026/06/30 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:34 [entrez] AID - S1569-9048(26)00128-X [pii] AID - 10.1016/j.resp.2026.104669 [doi] PST - aheadofprint SO - Respir Physiol Neurobiol. 2026 Oct 27:104669. doi: 10.1016/j.resp.2026.104669. PMID- 42946109 OWN - NLM STAT- Publisher LR - 20261027 IS - 1542-7714 (Electronic) IS - 1542-3565 (Linking) DP - 2026 Oct 27 TI - High burden of potentially undiagnosed MASH in a general population in Sweden. LID - S1542-3565(26)00811-6 [pii] LID - 10.1016/j.cgh.2026.10.010 [doi] AB - BACKGROUND AND AIMS: Few studies have explored the prevalence and outcomes of fibrotic metabolic dysfunction-associated steatohepatitis (MASH) in the general population. We aimed to characterize patients at risk of MASH using the validated Fibrotic NASH Index (FNI) and to compare the incidence of major adverse liver outcomes (MALO), major adverse cardiovascular events (MACE) and mortality between FNI risk groups and patients with established MASH. METHODS: Patients were classified as high risk (FNI>/=0.33 and >/= 1 cardiometabolic risk factor), or low-risk (FNI<0.1 +/- cardiometabolic risk factors) using laboratory data from routine clinical care in Stockholm, Sweden in 2010-2020. Patients with established MASH had a recorded ICD-10 code (K75.8) in Swedish specialist care (1998-2022). Data were analysed using Cox regression models and MALO, MACE and mortality were measured in number of events, cumulative incidence, and incidence rates. RESULTS: FNI was available in 162,456 patients, of which 23% were considered at high risk of MASH, and 39% considered low-risk. Among patients with established MASH (n=615), MALO's were more common than in high-risk FNI patients (45% vs 0.6%) at baseline. The rate of incident MALO was higher in diagnosed MASH patients vs the high-risk FNI group (adjusted HR [aHR]=6.73, 95%CI=4.03-11.23) and higher in high vs low-risk FNI group (aHR=4.6, 95%CI=3.9-5.4). The rate of MACE was also higher in high- vs low-risk (aHR=1.8, 95%CI=1.7-1.8) but not significantly different in high-risk vs diagnosed patients (aHR=0.6, 95%CI=0.3-1.2). CONCLUSION: There is a large burden of potentially undiagnosed at-risk MASH in patients from a general population with risk factors for MASH. Almost half of patients with established MASH already have MALO at baseline, reinforcing the need for earlier diagnosis. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Inc. All rights reserved. FAU - Grip, Emilie Toresson AU - Grip ET AD - Quantify Research AB, Hantverkargatan 8, 11221 Stockholm, Sweden; Department of Medicine, Huddinge, Karolinska Institutet, Stockholm, Sweden. Electronic address: emilie.toresson-grip@quantifyresearch.com. FAU - Basuroy, Ron AU - Basuroy R AD - Novo Nordisk Denmark A/S, Copenhagen Denmark. FAU - Johansen, Pierre AU - Johansen P AD - Novo Nordisk Denmark A/S, Copenhagen Denmark. FAU - Mangla, Kamal Kant AU - Mangla KK AD - Novo Nordisk Service Center India Pvt. Ltd., Bengaluru, India. FAU - Skroder, Helena AU - Skroder H AD - Quantify Research AB, Hantverkargatan 8, 11221 Stockholm, Sweden; Department of Medicine, Huddinge, Karolinska Institutet, Stockholm, Sweden. FAU - Presto, Jenny AU - Presto J AD - Novo Nordisk Scandinavia AB. FAU - Lazarus, Jeffrey V AU - Lazarus JV AD - CUNY Graduate School of Public Health and Health Policy, NYC, NY, USA; Barcelona Institute for Global Health (ISGlobal), Barcelona, Spain. FAU - Schattenberg, Jorn M AU - Schattenberg JM AD - Saarland University Medical Center, Department of Internal Medicine II, Homburg, Germany; Faculty of Medicine, Saarland University, Saarbrucken, Germany; PharmaScienceHub (PSH), Saarbrucken, Germany. FAU - Hagstrom, Hannes AU - Hagstrom H AD - Department of Medicine, Huddinge, Karolinska Institutet, Stockholm, Sweden; Division of Upper GI Diseases, Karolinska University Hospital, Stockholm, Sweden. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Clin Gastroenterol Hepatol JT - Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association JID - 101160775 SB - IM OTO - NOTNLM OT - MASH OT - MASLD OT - fatty liver OT - non-invasive test EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:34 PHST- 2025/04/24 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:34 [entrez] AID - S1542-3565(26)00811-6 [pii] AID - 10.1016/j.cgh.2026.10.010 [doi] PST - aheadofprint SO - Clin Gastroenterol Hepatol. 2026 Oct 27:S1542-3565(26)00811-6. doi: 10.1016/j.cgh.2026.10.010. PMID- 42946105 OWN - NLM STAT- Publisher LR - 20261027 IS - 1095-9130 (Electronic) IS - 1046-2023 (Linking) DP - 2026 Oct 27 TI - Comparison of cPCR, PCR-HRM, qPCR, and microarray for detecting GSTM1 and GSTT1 deletions and copy number variations: agreement and practical considerations. LID - S1046-2023(26)00215-X [pii] LID - 10.1016/j.ymeth.2026.10.010 [doi] AB - Copy number variations (CNVs) are clinically relevant as they may influence gene expression, enzymatic activity, and interindividual variability in drug response. Among these, deletions affecting the glutathione S-transferase (GST) genes GSTM1 and GSTT1 are of particular interest due to their potential implications for pharmacogenetic research. However, comparative evidence to guide the selection of the most appropriate method for CNV detection in different laboratory settings remains limited. Therefore, this study compared four molecular techniques, microarray, conventional PCR (cPCR), high-resolution melting PCR (PCR-HRM), and quantitative PCR (qPCR) for detecting CNVs in GSTM1 and GSTT1. Germline DNA samples from participants diagnosed with ovarian cancer were analyzed by all genotyping methods. A total of 80 participants were included for the cross-method agreement analysis. Agreement was assessed using Cohen's kappa coefficient, with 95% confidence intervals estimated using the Wilson score method. qPCR and microarray showed perfect concordance for both GSTM1 and GSTT1 qualitative and copy-number (CN) assessment. PCR-HRM showed perfect qualitative performance for GSTT1, whereas cPCR exhibited slightly lower agreement for both genes, despite maintaining high overall concordance. For those two genes, cPCR had the lowest entry cost, PCR-HRM combined qualitative robustness with workflow simplicity, qPCR was the most suitable targeted approach for CN estimation, and microarray was most advantageous when broader pharmacogenomic profiling was also intended. These findings indicate that method selection should be guided not only by analytical performance but also by the intended analytical objective and the available technical and financial resources. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Santos Fidelis, Giovana Fernanda AU - Santos Fidelis GF AD - School of Medical Sciences, Universidade Estadual de Campinas, Rua Vital Brasil, 80, Campinas, 13083894 Sao Paulo, Brazil. FAU - de Godoy Torso, Nadine AU - de Godoy Torso N AD - School of Medical Sciences, Universidade Estadual de Campinas, Rua Vital Brasil, 80, Campinas, 13083894 Sao Paulo, Brazil. FAU - Matos, Yasmim Gabriele AU - Matos YG AD - School of Medical Sciences, Universidade Estadual de Campinas, Rua Vital Brasil, 80, Campinas, 13083894 Sao Paulo, Brazil. FAU - Siguemoto, Julia Tiemi AU - Siguemoto JT AD - School of Medical Sciences, Universidade Estadual de Campinas, Rua Vital Brasil, 80, Campinas, 13083894 Sao Paulo, Brazil. FAU - Martins, Pedro Gamberini AU - Martins PG AD - School of Medical Sciences, Universidade Estadual de Campinas, Rua Vital Brasil, 80, Campinas, 13083894 Sao Paulo, Brazil. FAU - Mauricette Derchain, Sophie Francoise AU - Mauricette Derchain SF AD - Department of Obstetrics and Gynecology, Faculty of Medical Sciences, Universidade Estadual de Campinas, Rua Vital Brasil, 80, Campinas, 13083894 Sao Paulo, Brazil. FAU - Dagli-Hernandez, Carolina AU - Dagli-Hernandez C AD - School of Pharmaceutical Sciences, University of Sao Paulo (USP), Avenida Professor Lineu Prestes, 580, Sao Paulo 05508000 Sao Paulo, Brazil. FAU - de Carvalho Pincinato, Eder AU - de Carvalho Pincinato E AD - School of Medical Sciences, Universidade Estadual de Campinas, Rua Vital Brasil, 80, Campinas, 13083894 Sao Paulo, Brazil. FAU - Moriel, Patricia AU - Moriel P AD - Faculty of Pharmaceutical Sciences, Universidade Estadual de Campinas, Rua Candido Portinari, 200, Campinas 13083871, Sao Paulo, Brazil. Electronic address: morielpa@unicamp.br. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Methods JT - Methods (San Diego, Calif.) JID - 9426302 SB - IM OTO - NOTNLM OT - Copy number variations (CNV) OT - GSTM1 OT - GSTT1 OT - Glutathione S-transferases (GSTs) OT - Microarray OT - PCR-HRM OT - cPCR OT - qPCR COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Patricia Moriel reports financial support was provided by National Council for Scientific and Technological Development. Giovana Fernanda Santos Fidelis reports financial support was provided by State of Sao Paulo Research Foundation. Nadine de Godoy Torso reports financial support was provided by State of Sao Paulo Research Foundation. Yasmim Gabriele Matos reports financial support was provided by State of Sao Paulo Research Foundation. Julia Tiemi Siguemoto reports financial support was provided by State of Sao Paulo Research Foundation. Patricia Moriel reports financial support was provided by Coordination for the improvement of Higher Education Personnel. Patricia Moriel reports financial support was provided by Pharmaceutical Security Nucleus Project. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:34 PHST- 2026/07/28 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:34 [entrez] AID - S1046-2023(26)00215-X [pii] AID - 10.1016/j.ymeth.2026.10.010 [doi] PST - aheadofprint SO - Methods. 2026 Oct 27:S1046-2023(26)00215-X. doi: 10.1016/j.ymeth.2026.10.010. PMID- 42946104 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-5894 (Electronic) IS - 0730-725X (Linking) DP - 2026 Oct 27 TI - GlioVision: A multimodal MRI framework for non-invasive prediction of glioma molecular biomarkers. PG - 110829 LID - S0730-725X(26)00222-5 [pii] LID - 10.1016/j.mri.2026.110829 [doi] AB - Gliomas are primary brain tumors for which molecular biomarker assessment plays an important role in clinical evaluation and treatment planning. Traditional assessment relies on analysis of surgical tumor specimens, which carries procedural risks and sampling bias due to tumor heterogeneity. Existing deep learning methods can be computationally demanding and are often trained on narrowly represented datasets, which may limit their clinical deployment. In this study, we propose GlioVision, a multi-modal deep learning framework for non-invasive prediction of glioma molecular biomarkers using multi-parametric MRI, including T1, T1-Gd, T2, and FLAIR sequences. The framework employs a 3D DenseNet-based architecture with channel and spatial feature recalibration, combined with an adaptive processing module that handles variability across multi-institutional datasets. In addition, a confidence-based filtering mechanism (CFPM) is introduced to identify low-confidence predictions and quantify prediction uncertainty. The model is trained and evaluated on a multi-cohort dataset compiled from publicly available sources, comprising 2270 subjects with heterogeneous imaging protocols. Subject-level splitting is used to prevent data leakage, and performance is reported using AUC, accuracy, sensitivity, and specificity across all biomarkers. Independent public cohorts were used for testing. GlioVision achieves AUC values of 0.94 for IDH, 0.87 for 1p/19q, 0.86 for MGMT, and 0.92 for tumor grade, indicating consistent performance across biomarkers. The effect of confidence-based filtering is also analyzed to examine the trade-off between prediction coverage and accuracy. Finally, we investigate a simple model obfuscation approach based on controlled image perturbations and evaluate its impact on prediction performance. This study presents a multi-institutional evaluation of a deep learning pipeline for predicting glioma biomarkers from MRI. It may support further research in radiogenomic analysis and non-invasive tumor characterization. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Nazir, Anam AU - Nazir A AD - Department of Health Data Science and Artificial Intelligence, D. Bradley McWilliams School of Biomedical Informatics, The University of Texas Health Science Center, Houston, TX, 77030, USA. Electronic address: Anam.Nazir@uth.tmc.edu. FAU - Cheema, Muhammad Nadeem AU - Cheema MN AD - Department of Health Data Science and Artificial Intelligence, D. Bradley McWilliams School of Biomedical Informatics, The University of Texas Health Science Center, Houston, TX, 77030, USA. FAU - Hsu, Yu-Chun AU - Hsu YC AD - Department of Health Data Science and Artificial Intelligence, D. Bradley McWilliams School of Biomedical Informatics, The University of Texas Health Science Center, Houston, TX, 77030, USA. FAU - Jiang, Xiaoqian AU - Jiang X AD - Department of Health Data Science and Artificial Intelligence, D. Bradley McWilliams School of Biomedical Informatics, The University of Texas Health Science Center, Houston, TX, 77030, USA. FAU - Zhu, Jay-Jiguang AU - Zhu JJ AD - Department of Neurosurgery, The University of Texas Health Science Center, Houston, TX, 77030, USA. FAU - Harmanci, Akdes Serin AU - Harmanci AS AD - Department of Neurosurgery, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Harmanci, Arif AU - Harmanci A AD - Department of Health Data Science and Artificial Intelligence, D. Bradley McWilliams School of Biomedical Informatics, The University of Texas Health Science Center, Houston, TX, 77030, USA. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Magn Reson Imaging JT - Magnetic resonance imaging JID - 8214883 SB - IM OTO - NOTNLM OT - Glioblastoma OT - IDH mutation OT - MONAI OT - Magnetic Resonance Imaging (MRI) OT - Non-invasive glioma molecular biomarker prediction OT - The Cancer Imaging Archive (TCIA) COIS- Declaration of competing interest The authors declare that they have no conflicts of interest. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:34 PHST- 2026/07/13 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:34 [entrez] AID - S0730-725X(26)00222-5 [pii] AID - 10.1016/j.mri.2026.110829 [doi] PST - aheadofprint SO - Magn Reson Imaging. 2026 Oct 27:110829. doi: 10.1016/j.mri.2026.110829. PMID- 42946096 OWN - NLM STAT- Publisher LR - 20261027 IS - 1879-0720 (Electronic) IS - 0928-0987 (Linking) DP - 2026 Oct 27 TI - Machine learning-empowered nanomaterial design and application for tear fluid-based point-of-care tests: from material discovery to clinical decision-making. PG - 107698 LID - S0928-0987(26)00272-1 [pii] LID - 10.1016/j.ejps.2026.107698 [doi] AB - Tear fluid harbors a rich array of cellular and molecular biomarkers that mirror both ocular and systemic health, positioning it as an ideal medium for non-invasive point-of-care testing (POCT). Yet its analytical utility is constrained by sub-microliter sample volumes, dynamic compositional fluctuations, and trace-level biomarker concentrations. Nanomaterials, with their surface-to-volume ratio, programmable surface chemistry, and tunable optical and catalytic properties, have been engineered into miniaturized platforms to enable target enrichment, multiplexed recognition, and signal amplification within microliter samples. Building on this material foundation, machine learning (ML) is now reshaping the entire analytical workflow. Data-driven algorithms accelerate the in-silico discovery and rational optimization of functional nanomaterials. Deep learning models further decouple matrix interference to enable ultra-sensitive, multiplexed readouts, while downstream algorithms translate multi-biomarker profiles into actionable clinical decisions. This review summarizes design strategies for ML-empowered, tear fluid-based POCT and examines how nanomaterials address the inherent constraints of tear analysis. However, clinical translation remains limited by non-standardized tear collection and data acquisition, insufficient large-scale clinical validation, and concerns regarding algorithm robustness and interpretability. The review further outlines translational applications spanning disease diagnosis, patient stratification, therapeutic monitoring, and decentralized longitudinal surveillance to support biomarker-driven precision medicine. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Chen, Yichu AU - Chen Y AD - Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China. FAU - Xu, Tian AU - Xu T AD - Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China. FAU - Wu, Yu AU - Wu Y AD - State Key Laboratory of Eye Health, Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China; Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, 200011, China. FAU - Liu, Wanshan AU - Liu W AD - State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University, Shanghai 200030, P. R. China. FAU - Qian, Kun AU - Qian K AD - State Key Laboratory of Systems Medicine for Cancer, School of Biomedical Engineering, Institute of Medical Robotics and Shanghai Academy of Experimental Medicine, Shanghai Jiao Tong University, Shanghai 200030, P. R. China. Electronic address: k.qian@sjtu.edu.cn. FAU - Su, Yun AU - Su Y AD - State Key Laboratory of Eye Health, Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China; Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China; Shanghai Key Laboratory of Orbital Diseases and Ocular Oncology, Shanghai, 200011, China. Electronic address: suyun_sy@shsmu.edu.cn. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Netherlands TA - Eur J Pharm Sci JT - European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences JID - 9317982 SB - IM OTO - NOTNLM OT - Biomarkers OT - Machine learning OT - Nanomaterials OT - Point-of-care testing OT - Precision medicine OT - Tear fluid COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:33 PHST- 2026/06/12 00:00 [received] PHST- 2026/08/04 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:33 [entrez] AID - S0928-0987(26)00272-1 [pii] AID - 10.1016/j.ejps.2026.107698 [doi] PST - aheadofprint SO - Eur J Pharm Sci. 2026 Oct 27:107698. doi: 10.1016/j.ejps.2026.107698. PMID- 42946093 OWN - NLM STAT- Publisher LR - 20261029 IS - 1873-6971 (Electronic) IS - 0367-326X (Linking) VI - 195 DP - 2026 Oct 27 TI - Triazole-based structural modification of plant- and fungal-derived natural products: recent advances in bioactivity and medicinal potential. PG - 107532 LID - S0367-326X(26)00452-1 [pii] LID - 10.1016/j.fitote.2026.107532 [doi] AB - Plant- and fungal-derived natural products remain important sources of bioactive scaffolds for pharmacological research and lead discovery. However, their further development may be limited by poor aqueous solubility, metabolic instability, moderate potency, low bioavailability, or insufficient target selectivity. Triazole-based structural modification, particularly 1,2,3-triazole formation via click chemistry, provides a versatile strategy for diversifying natural-product scaffolds and optimizing their biological properties. This review critically summarizes studies published from June 2021 to April 2026 on the design, synthesis, biological activities, and structure-activity relationships of triazole-modified natural products and related scaffolds derived mainly from plants and fungi. Representative terpenoids, steroids, flavonoids, coumarins, chalcones, curcuminoids, lignans, alkaloids, phenolics, and fungal metabolites are comparatively discussed across anticancer, antimicrobial, anti-inflammatory, antiviral, antiparasitic, neuroprotective and alzheimer's disease-related activities, antidiabetic, and phytotoxic activities. Particular emphasis is placed on the influence of the position of triazole incorporation, substitution patterns, linker characteristics, and parent scaffolds on biological activity, together with comparisons with parent compounds and positive controls where available. Overall, triazole incorporation frequently improved biological activity within individual derivative series; however, direct cross-study comparisons remain limited by differences in biological models and assay conditions. Moreover, most reported compounds remain at the in vitro stage, with limited target validation, in vivo efficacy, pharmacokinetic, metabolic, and long-term toxicity data. This review provides a critical medicinal-chemistry perspective on recent triazole-modified natural products and highlights structural features and evidence gaps relevant to their further development as bioactive leads. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Cui, Nan-Nan AU - Cui NN AD - Key Laboratory of Natural Medicines of the Changbai Mountain (Yanbian University), Ministry of Education, China. FAU - Cui, Cheng-Mei AU - Cui CM AD - Key Laboratory of Natural Medicines of the Changbai Mountain (Yanbian University), Ministry of Education, China. FAU - Guo, Hong-Yan AU - Guo HY AD - Key Laboratory of Natural Medicines of the Changbai Mountain (Yanbian University), Ministry of Education, China. Electronic address: hongyanguo@ybu.edu.cn. FAU - Jin, Chun-Mei AU - Jin CM AD - Key Laboratory of Natural Medicines of the Changbai Mountain (Yanbian University), Ministry of Education, China. Electronic address: cmijin@ybu.edu.cn. FAU - Quan, Zhe-Shan AU - Quan ZS AD - Key Laboratory of Natural Medicines of the Changbai Mountain (Yanbian University), Ministry of Education, China. Electronic address: zsquan@ybu.edu.cn. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Netherlands TA - Fitoterapia JT - Fitoterapia JID - 16930290R SB - IM OTO - NOTNLM OT - Bioactivity OT - Fungal metabolites OT - Medicinal plants OT - Natural products OT - Structural modification OT - Triazole COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:33 PHST- 2026/05/21 00:00 [received] PHST- 2026/09/02 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 20:33 [entrez] AID - S0367-326X(26)00452-1 [pii] AID - 10.1016/j.fitote.2026.107532 [doi] PST - aheadofprint SO - Fitoterapia. 2026 Oct 27;195:107532. doi: 10.1016/j.fitote.2026.107532. PMID- 42946092 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-3304 (Electronic) IS - 0039-6257 (Linking) DP - 2026 Oct 27 TI - Fundus torsion in strabismus: Measurement, clinical interpretation, and emerging imaging approaches. LID - S0039-6257(26)00155-4 [pii] LID - 10.1016/j.survophthal.2026.10.013 [doi] AB - Fundus torsion is an important but underused anatomical marker in strabismus evaluation, particularly for cyclovertical disorders. Unlike subjective cyclodeviation testing, fundus-based assessment provides an objective reference based on retinal landmarks, though it remains influenced by image acquisition, head position, ocular anatomy, and measurement conventions. We synthesize the anatomical basis of ocular and fundus torsion, contemporary qualitative and quantitative assessment methods, and disease-specific evidence across superior oblique palsy, pattern strabismus, pediatric and adult strabismus, and surgical follow-up. The review emphasizes 3 clinically important issues: the frequent dissociation between objective fundus torsion and subjective cyclodeviation, the need for method-specific interpretation using reproducibility and minimal-detectable-change data, and the importance of avoiding isolated treatment decisions based on anatomical torsion alone. Recent optical coherence tomography-based and artificial intelligence-assisted approaches may improve measurement consistency and scalability, but current evidence supports their use as adjunctive tools rather than autonomous decision systems. Fundus torsion should therefore be interpreted within a multimodal clinical framework that integrates motility findings, sensory status, age, chronicity, fixation behavior, and surgical history. Treatment options include observation and symptom relief in selected patients, inferior oblique weakening for associated overaction, and torsion-directed surgery for persistent symptomatic excyclotorsion; comparative evidence remains limited. Prospective comparative treatment studies are a clinical research priority. Standardized acquisition protocols, explicit reporting conventions, age- and population-specific normative data, externally validated automated methods, and prospective studies linking torsional change to symptoms and functional outcomes are needed to strengthen the clinical utility of fundus torsion assessment. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Jin, Kai AU - Jin K AD - Eye Center of Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China; Zhejiang Provincial Key Laboratory of Ophthalmology, Zhejiang Provincial Clinical Research Center for Eye Diseases, Zhejiang Provincial Engineering Institute on Eye Diseases, Hangzhou, Zhejiang, China. Electronic address: jinkai@zju.edu.cn. FAU - Yip, Chee Chew AU - Yip CC AD - Department of Ophthalmology & Visual Sciences, Khoo Teck Puat Hospital, Singapore, Singapore; Yong Loo Lin School of Medicine, National University of Singapore, Singapore; Ophthalmology Department, National University Hospital, Singapore. FAU - Christiansen, Stephen P AU - Christiansen SP AD - Department of Ophthalmology & Vision Sciences, Boston Medical Center, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts, USA; Department of Pediatrics, Boston Medical Center, Boston University Chobanian & Avedisian School of Medicine, Boston, Massachusetts, USA. FAU - Grzybowski, Andrzej AU - Grzybowski A AD - Institute for Research in Ophthalmology, Foundation for Ophthalmology Development, Poznan, Poland; Department of Ophthalmology, University of Warmia and Mazury, Olsztyn, Poland. FAU - Jiang, Jingjing AU - Jiang J AD - Department of Ophthalmology, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Key Laboratory of Major Diseases in Children, Ministry of Education, Beijing, China. Electronic address: jiangjingjing@mail.ccmu.edu.cn. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - United States TA - Surv Ophthalmol JT - Survey of ophthalmology JID - 0404551 SB - IM OTO - NOTNLM OT - Cyclodeviation OT - Disc-fovea angle OT - Fundus torsion OT - Ocular torsion OT - Strabismus COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:33 PHST- 2026/06/17 00:00 [received] PHST- 2026/10/20 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 20:33 [entrez] AID - S0039-6257(26)00155-4 [pii] AID - 10.1016/j.survophthal.2026.10.013 [doi] PST - aheadofprint SO - Surv Ophthalmol. 2026 Oct 27:S0039-6257(26)00155-4. doi: 10.1016/j.survophthal.2026.10.013. PMID- 42946091 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-2259 (Electronic) IS - 0168-9452 (Linking) DP - 2026 Oct 27 TI - GhHDZ-87, a cotton HD-Zip transcription factor, involved in regulating salt stress tolerance in G. hirsutum. PG - 113558 LID - S0168-9452(26)00586-8 [pii] LID - 10.1016/j.plantsci.2026.113558 [doi] AB - Salt stress severely constrains cotton seedling establishment, yield, and fiber quality. Although HD‑Zip transcription factors are known to play essential roles in abiotic stress adaptation in various plant species, their functions in cotton remain largely unexplored. In this study, we performed a comprehensive genome‑wide analysis of HD‑Zip genes in Gossypium hirsutum to uncover their evolutionary history and identify stress‑responsive regulatory components. Genome‑wide screening identified 90 HDZ members that were unevenly distributed across the 26 chromosomes of upland cotton. Phylogenetic analysis showed that the upland cotton HDZs were divided into six groups, with clade B absent from the seven clades identified across species. Analyses of gene structure and conserved domains provided strong evidence for the evolutionary conservation of HDZ members. The expansion of the GhHDZ family was primarily driven by duplication events, particularly whole‑genome duplication (WGD) and allotetraploidization, with purifying selection acting on these genes. The expression of GhHDZ-87 was upreglated under salt, drought, and ABA treatments. beta-Glucuronidase (GUS) staining indicated that GhHDZ-87 was expressed in the roots, stems and leaves of ProGhHDZ-87::GUS transgenic Arabidopsis thaliana. VIGS(Virus-induced gene silencing) assays suggested that knockdown of GhHDZ‑87 reduced salt tolerance in cotton, potentially via ABA signaling and ion‑homeostasis pathways. Taken together, this study comprehensively characterized the GhHDZ gene family in cotton and identified GhHDZ-87 as a positive regulator of salt tolerance. This study not only provides genetic resources and theoretical support for improving salt tolerant in cotton, but also improves our understanding of the regulatory pathways underlying salt tolerance. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Zhang, Qi AU - Zhang Q AD - College of Life Sciences, Luoyang Normal University, Luoyang, China. FAU - Kang, Meng AU - Kang M AD - State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China. FAU - Zhang, Jingjing AU - Zhang J AD - Department of Horticulture and Landscape Architecture, Taiyuan University, Taiyuan 030032, China. FAU - Guo, Xiaohao AU - Guo X AD - State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China. FAU - Jian, Hongliang AU - Jian H AD - State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China. FAU - An, Li AU - An L AD - State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China. FAU - Sun, Mengxi AU - Sun M AD - State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China. FAU - Wu, Hongmei AU - Wu H AD - State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China. FAU - Wei, Fei AU - Wei F AD - College of Life Sciences, Luoyang Normal University, Luoyang, China. Electronic address: weifei888000@outlook.com. FAU - Lu, Jianhua AU - Lu J AD - State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China. Electronic address: lujh212760@163.com. FAU - Fu, Xiaokang AU - Fu X AD - State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China. Electronic address: xkang_2010@163.com. LA - eng PT - Journal Article DEP - 20261027 PL - Ireland TA - Plant Sci JT - Plant science : an international journal of experimental plant biology JID - 9882015 SB - IM OTO - NOTNLM OT - Evolutionary conservation OT - GhHDZ-87 OT - Gossypium hirsutum OT - HD‑Zip gene family OT - Salt tolerance COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Declaration of Competing Interest We declare that we have no financial and personal relationships with other people or organizations that could inappropriately influence our work, and there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as inffuencing the position presented in, or the review of, the manuscript entitled. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:33 PHST- 2026/06/10 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:33 [entrez] AID - S0168-9452(26)00586-8 [pii] AID - 10.1016/j.plantsci.2026.113558 [doi] PST - aheadofprint SO - Plant Sci. 2026 Oct 27:113558. doi: 10.1016/j.plantsci.2026.113558. PMID- 42946085 OWN - NLM STAT- Publisher LR - 20261027 IS - 2451-9030 (Electronic) IS - 2451-9022 (Linking) DP - 2026 Oct 27 TI - Did I do that? Efficacy estimation distinguishes rumination and anhedonia symptomatology. LID - S2451-9022(26)00315-0 [pii] LID - 10.1016/j.bpsc.2026.10.010 [doi] AB - Accurately tracking the effectiveness of one's effort is critical for adaptive behavior. However, this process may be altered in rumination and anhedonia, two symptoms that have been associated with failures in low-level cognitive processes that are relevant to the estimation of effort efficacy METHODS: Here, we empirically tested this question in an online sample (N=611), where participants completed an efficacy tracking task where they had to estimate the efficacy of their effort in environments where true efficacy was either low or high RESULTS: Participants with high-rumination and low-anhedonia showed selectively impaired efficacy estimation in low-efficacy contexts, consistent with excessive self-attribution when control is objectively limited. In contrast, higher anhedonia predicted an under-attribution of efficacy across conditions, characterized by a disproportionate learning from non-performance-based feedback. Pre-registered robustness and sensitivity analyses supported effect stability CONCLUSIONS: These findings suggest that rumination and anhedonia are associated with dissociable disruptions in efficacy estimation and tracking - over-attribution of control in low-efficacy environments versus a generalized under-attribution of efficacy - providing mechanistic support for distinct computational profiles underlying these transdiagnostic symptoms. Implications for precision intervention and future research in computational psychiatry are discussed. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Migo, Marta AU - Migo M AD - Emory University, Department of Psychology, 30322, Atlanta, GA. FAU - Brown, Vanessa M AU - Brown VM AD - Emory University, Department of Psychology, 30322, Atlanta, GA. FAU - Cooper, Jessica A AU - Cooper JA AD - Emory University School of Medicine, Department of Psychiatry and Behavioral Sciences, 30322, Atlanta, GA. FAU - Ryan, Jonathan AU - Ryan J AD - Emory University, Department of Psychology, 30322, Atlanta, GA. FAU - Hitchcock, Peter F AU - Hitchcock PF AD - Emory University, Department of Psychology, 30322, Atlanta, GA. FAU - Treadway, Michael T AU - Treadway MT AD - Emory University, Department of Psychology, 30322, Atlanta, GA; Emory University School of Medicine, Department of Psychiatry and Behavioral Sciences, 30322, Atlanta, GA. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Biol Psychiatry Cogn Neurosci Neuroimaging JT - Biological psychiatry. Cognitive neuroscience and neuroimaging JID - 101671285 SB - IM OTO - NOTNLM OT - Anhedonia OT - Behavioral Phenotyping OT - Efficacy-Tracking OT - Rumination EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:33 PHST- 2026/06/16 00:00 [received] PHST- 2026/09/04 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:33 [entrez] AID - S2451-9022(26)00315-0 [pii] AID - 10.1016/j.bpsc.2026.10.010 [doi] PST - aheadofprint SO - Biol Psychiatry Cogn Neurosci Neuroimaging. 2026 Oct 27:S2451-9022(26)00315-0. doi: 10.1016/j.bpsc.2026.10.010. PMID- 42946082 OWN - NLM STAT- Publisher LR - 20261027 IS - 1878-1810 (Electronic) IS - 1878-1810 (Linking) DP - 2026 Oct 27 TI - MRI-visible CDDO-Me@AL/HA hydrogel for theranostics of radiation-induced intestinal injury. LID - S1931-5244(26)00220-3 [pii] LID - 10.1016/j.trsl.2026.10.010 [doi] AB - Radiation-induced intestinal injury (RIII) is a serious complication of abdominal and pelvic radiotherapy. Its mechanism is complex, and traditional treatment methods are difficult to achieve precise intervention and efficacy visualization. Bardoxolone methyl (CDDO-Me) has a therapeutic effect on intestinal injury as an Nrf2 activator. However, there are limitations such as uncontrolled drug release and lack of efficacy visualization. To address this problem, we prepared a sodium alginate/hyaluronic acid (AL/HA) pH-responsive oral hydrogel loaded with CDDO-Me, and introduced melanin nanoparticles (SMNP) with enhanced MRI signal to achieve non-invasive visualization of the distribution, localization and retention of the hydrogel in rats. The hydrogel exhibited resistance to gastric acid, mucoadhesive properties, and prolonged retention in the small intestine and colon, thus achieving controlled intestinal release of drugs. In addition, our research suggested that the oral CDDO-Me@AL/HA@SMNP hydrogel could alleviate intestinal oxidative stress injury and down-regulate pro-inflammatory cytokines in mouse models. These effects may be associated with Nrf2 activation and ROS clearance, and may also involve the PI3K/Akt/GSK3beta pathway. Overall, our research indicated that the CDDO-Me@AL/HA@SMNP hydrogel integrates therapeutic efficacy with MRI-based visualization/tracking capabilities for RIII. These findings represent a preclinical proof-of-concept, and further optimization, safety evaluation, and translational studies are required before any potential clinical application. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Yang, Siyu AU - Yang S AD - Department of Radiology, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan, Kunming 650118, Yunnan, China. FAU - Li, Qinqing AU - Li Q AD - Department of Radiology, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan, Kunming 650118, Yunnan, China; Department of Radiology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650033, Yunnan, China. FAU - Jin, Fuwei AU - Jin F AD - Department of Radiology, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan, Kunming 650118, Yunnan, China. FAU - Zhang, Nan AU - Zhang N AD - Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Gastrointestinal Surgery IV, Peking University Cancer Hospital & Institute, Beijing 100142, China. FAU - Ran, Fengming AU - Ran F AD - Department of Pathology, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan, Kunming 650118, Yunnan, China. FAU - Deng, Lijuan AU - Deng L AD - Department of Radiology, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan, Kunming 650118, Yunnan, China. FAU - Niu, Xiaofeng AU - Niu X AD - Department of Radiology, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan, Kunming 650118, Yunnan, China. FAU - Ren, Zheng AU - Ren Z AD - Department of Radiology, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan, Kunming 650118, Yunnan, China. FAU - Chen, Yucheng AU - Chen Y AD - Department of Endocrinology, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan, China. FAU - Ye, Ye AU - Ye Y AD - Department of Radiology, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan, Kunming 650118, Yunnan, China. FAU - Wang, Jiaping AU - Wang J AD - Department of Radiology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650033, Yunnan, China. Electronic address: jiapingwang12@163.com. FAU - Yang, Jun AU - Yang J AD - Department of Radiology, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan, Kunming 650118, Yunnan, China. Electronic address: yangjun@kmmu.edu.cn. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Transl Res JT - Translational research : the journal of laboratory and clinical medicine JID - 101280339 SB - IM OTO - NOTNLM OT - Bardoxolone methyl OT - Magnetic resonance imaging OT - Melanin nanoparticles OT - Oral hydrogel OT - Radiation-induced intestinal injury COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:33 PHST- 2026/06/08 00:00 [received] PHST- 2026/10/26 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:33 [entrez] AID - S1931-5244(26)00220-3 [pii] AID - 10.1016/j.trsl.2026.10.010 [doi] PST - aheadofprint SO - Transl Res. 2026 Oct 27:S1931-5244(26)00220-3. doi: 10.1016/j.trsl.2026.10.010. PMID- 42946080 OWN - NLM STAT- Publisher LR - 20261027 IS - 1096-0805 (Electronic) IS - 0022-2011 (Linking) DP - 2026 Oct 27 TI - Evidence of Ecytonucleospora hepatopenaei in environmental reservoirs and macrofaunal carriers from shrimp aquaculture ecosystems of south West India. PG - 108781 LID - S0022-2011(26)00257-0 [pii] LID - 10.1016/j.jip.2026.108781 [doi] AB - Ecytonucleospora hepatopenaei (EHP) is an emerging microsporidian parasite that causes growth retardation and reduced production in shrimp aquaculture. Transmission is considered to occur mainly through horizontal pathways, including ingestion of spores released by infected shrimp or exposure to contaminated environmental material. However, data on occurrence of EHP DNA in environmental matrices and potential macrofaunal carriers along the Karnataka coast are limited. This study assessed the presence of EHP DNA in water, sediment, seaweeds, and mollusks from shrimp farms and surrounding aquatic environments. Among the 124 samples screened by species-specific PCR, EHP DNA was detected in 78 (62.9%) across all sample types. These results support the presence of EHP-associated DNA in both macrofauna and environmental matrices in aquaculture environments, but the limited sample sizes preclude strong conclusions about relative detection rates or the strength of any association between EHP DNA and sample types. Phylogenetic analysis of SSU rRNA sequences showed close clustering with previously reported Indian EHP isolates. Calcofluor white and Giemsa-stained sediment samples revealed putative microsporidian spore-like structures. These findings demonstrate widespread detection of EHP -associated DNA in environmental matrices and selected macrofauna. The results underscore the need for comprehensive management in shrimp farming, including regular monitoring, strict biosecurity, and monitoring of wild macrofauna and other potential environmental sources. Such measures are essential to mitigate EHP impact and support sustainable, profitable aquaculture on India's west coast. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Shah, Rajesh Kumar AU - Shah RK AD - Nitte (Deemed to be University), Department of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Deralakatte, Mangaluru 575018, Karnataka, India. FAU - Prithvisagar, Kattapuni Suresh AU - Prithvisagar KS AD - Nitte (Deemed to be University), Department of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Deralakatte, Mangaluru 575018, Karnataka, India. FAU - Rai, Praveen AU - Rai P AD - Nitte (Deemed to be University), Department of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Deralakatte, Mangaluru 575018, Karnataka, India. FAU - Karunasagar, Indrani AU - Karunasagar I AD - Nitte (Deemed to be University), FAO Reference Centre for AMR and Aquaculture Biosecurity, Deralakatte, Mangaluru 575018, Karnataka, India. FAU - Kumar, Ballamoole Krishna AU - Kumar BK AD - Nitte (Deemed to be University), Department of Infectious Diseases and Microbial Genomics, Nitte University Centre for Science Education and Research, Deralakatte, Mangaluru 575018, Karnataka, India. Electronic address: krishnakumarb@nitte.edu.in. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Invertebr Pathol JT - Journal of invertebrate pathology JID - 0014067 SB - IM OTO - NOTNLM OT - Carriers OT - Ecytonucleospora hepatopenaei OT - Environmental DNA OT - Macrofauna matrices OT - Prevalence OT - Shrimp aquaculture COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:33 PHST- 2026/07/10 00:00 [received] PHST- 2026/10/23 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:33 [entrez] AID - S0022-2011(26)00257-0 [pii] AID - 10.1016/j.jip.2026.108781 [doi] PST - aheadofprint SO - J Invertebr Pathol. 2026 Oct 27:108781. doi: 10.1016/j.jip.2026.108781. PMID- 42946072 OWN - NLM STAT- Publisher LR - 20261027 IS - 1097-6809 (Electronic) IS - 0741-5214 (Linking) DP - 2026 Oct 27 TI - Long-term patency outcomes after first autogenous arteriovenous fistula creation in patients aged 80 years or older: a competing-risk analysis. LID - S0741-5214(26)01779-9 [pii] LID - 10.1016/j.jvs.2026.10.024 [doi] AB - OBJECTIVE: Whether chronological age predicts arteriovenous fistula (AVF) durability remains uncertain. We compared primary patency between patients aged >/=80 and <80 years using competing-risk analysis and examined relevant vascular characteristics. METHODS: This single-center retrospective cohort included 416 consecutive patients undergoing first autogenous AVF creation during 2017-2020, with follow-up through March 2025. Intraoperative vessel diameters, macroscopic arterial atherosclerosis and preoperative ankle-brachial and toe-brachial indices were prospectively recorded. Death before primary patency loss was treated as a competing event. Cumulative incidence was estimated using Aalen-Johansen and compared using Gray's test. Associations were assessed using Fine-Gray and cause-specific Cox regression, with age modeled continuously in a sensitivity analysis. RESULTS: Among 416 patients, 109 (26.2%) were aged >/=80 years. Median follow-up was 48 months. Older patients had more macroscopic arterial atherosclerosis (67.0% vs 47.9%; P<.001), lower ankle-brachial (1.09 vs 1.16; P<.001) and toe-brachial indices (0.69 vs 0.76; P=.002), and more venous diameters <2.5 mm (22.0% vs 13.4%; P=.030). The cumulative incidence of primary patency loss did not differ between groups (Gray P = .710), with 36-month estimates of 43.2% and 38.0%. Age >/=80 years was not independently associated with primary patency loss (subdistribution hazard ratio [sHR], 1.12; 95% CI, 0.74-1.70), whereas each 10-year increase was associated with higher risk in a sensitivity analysis (sHR, 1.20; 95% CI, 1.04-1.39). Proximal anastomosis was associated with lower risk (sHR, 0.57; 95% CI, 0.37-0.88), and venous diameter <2.5 mm with higher risk (sHR, 1.70; 95% CI, 1.05-2.75). Assisted primary and secondary patency did not differ. Fewer than 10% of fistulas were permanently abandoned by 60 months in either group, and 60-month survival among older patients was 82.0%. CONCLUSIONS: Among patients selected for autogenous AVF creation, age >/=80 years was not independently associated with primary patency loss, whereas a continuous-age sensitivity analysis suggested a modest increase in risk per decade. These findings suggest that an age threshold alone should not determine AVF candidacy in otherwise suitable patients, although interpretation is tempered by loss to follow-up. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Doita, Tsutomu AU - Doita T AD - Department of Vascular Surgery, Japan Organization of Occupational Health and Safety, Osaka Rosai Hospital, Sakai, Osaka, Japan; Department of Cardiovascular Surgery, the University of Osaka Graduate School of Medicine, Suita, Osaka, Japan. FAU - Nakamura, Takashi AU - Nakamura T AD - Department of Vascular Surgery, Japan Organization of Occupational Health and Safety, Osaka Rosai Hospital, Sakai, Osaka, Japan; Department of Vascular Surgery, Ishikiri Seiki Hospital, Higashiosaka, Osaka, Japan. Electronic address: takashin@mvd.biglobe.ne.jp. FAU - Yamakura, Takuya AU - Yamakura T AD - Department of Vascular Surgery, Japan Organization of Occupational Health and Safety, Osaka Rosai Hospital, Sakai, Osaka, Japan. FAU - Yamasumi, Taro AU - Yamasumi T AD - Department of Vascular Surgery, Japan Organization of Occupational Health and Safety, Osaka Rosai Hospital, Sakai, Osaka, Japan; Department of Cardiovascular Surgery, the University of Osaka Graduate School of Medicine, Suita, Osaka, Japan. FAU - Miyake, Keisuke AU - Miyake K AD - Department of Cardiovascular Surgery, the University of Osaka Graduate School of Medicine, Suita, Osaka, Japan. FAU - Miyagawa, Shigeru AU - Miyagawa S AD - Department of Cardiovascular Surgery, the University of Osaka Graduate School of Medicine, Suita, Osaka, Japan. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Vasc Surg JT - Journal of vascular surgery JID - 8407742 SB - IM OTO - NOTNLM OT - arteriovenous fistula OT - competing risk OT - octogenarian OT - primary patency OT - vascular access EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:33 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:33 [entrez] AID - S0741-5214(26)01779-9 [pii] AID - 10.1016/j.jvs.2026.10.024 [doi] PST - aheadofprint SO - J Vasc Surg. 2026 Oct 27:S0741-5214(26)01779-9. doi: 10.1016/j.jvs.2026.10.024. PMID- 42946070 OWN - NLM STAT- Publisher LR - 20261027 IS - 1097-6809 (Electronic) IS - 0741-5214 (Linking) DP - 2026 Oct 27 TI - Femoral Artery Access Outcomes and Technical Considerations in Vascular Ehlers-Danlos Syndrome. LID - S0741-5214(26)01778-7 [pii] LID - 10.1016/j.jvs.2026.10.023 [doi] AB - OBJECTIVE: Femoral artery access in vascular Ehlers-Danlos syndrome (VEDS) poses unique technical challenges due to structurally fragile arteries. This study evaluated femoral access-related complications in patients with genetically confirmed VEDS and sought to identify procedural and biologic factors that may inform access planning for endovascular procedures. METHODS: A cross-sectional analysis of patients with pathogenic/likely pathogenic COL3A1 variants who underwent endovascular procedures requiring femoral artery access. Data were obtained from the global GEN-VEDS Natural History Study and a retrospective single center cohort. Demographics, genotype, procedural indication, access and closure techniques, access-related complications, and outcomes were abstracted from clinical record. Major access-related complications were defined as femoral artery dissection, pseudoaneurysm, rupture, or closure device failure requiring open operative repair. RESULTS: A total of 117 femoral artery access sites were used in 105 endovascular interventions among 60 patients between 1989 and 2025. Median age at intervention was 39 years, and 53.3% of patients were male. Among 99 procedures with known access modality, 84 were performed percutaneously and 15 involved planned open femoral exposure. Manual compression was used in 33 percutaneous access sites, with no major access-related complications. Percutaneous closure devices were used in 39 access sites; major complications occurred in 5 access sites across 5 procedures, all requiring unplanned surgical exposure and repair. One additional percutaneous access site required unplanned open repair for access-related arterial injury and external iliac artery dissection before any closure device was attempted. Of percutaneous closure device failures, Perclose ProGlide and Starclose had the highest rate of failure (18.8% and 33.3% respectively). Three of four affected patients had large amino acid substitutions or exon skip mutations; the remaining patient had a small amino acid substitution but underwent 16-Fr sheath placement. No access-related complications occurred after planned open exposure. CONCLUSIONS: Femoral artery access in VEDS requires deliberate individualized planning. In this cohort, manual compression for smaller-caliber access (<6 Fr) was associated with favorable outcomes, whereas major complications occurred predominantly following attempted percutaneous closure. Planned open exposure, despite use in procedures involving larger sheath sizes, was not associated with access-related complications and may warrant consideration in selected circumstances, including procedures requiring larger sheath sizes, known prior closure-related arterial injury, and high-risk genotypes. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Lian, Tiffany AU - Lian T AD - Division of Vascular and Endovascular Surgery. Department of Surgery. Oregon Health and Sciences University. Portland, OR, USA. FAU - Davis, Alden AU - Davis A AD - Department of Interventional Radiology. Oregon Health and Sciences University. Portland, OR, USA. FAU - Griffith, Garin AU - Griffith G AD - Division of Vascular and Endovascular Surgery. Department of Surgery. Oregon Health and Sciences University. Portland, OR, USA. FAU - Minoza, Karen AU - Minoza K AD - Division of Vascular and Endovascular Surgery. Department of Surgery. Oregon Health and Sciences University. Portland, OR, USA. FAU - Kaufman, Claire AU - Kaufman C AD - Department of Interventional Radiology. Oregon Health and Sciences University. Portland, OR, USA. FAU - Shalhub, Sherene AU - Shalhub S AD - Division of Vascular and Endovascular Surgery. Department of Surgery. Oregon Health and Sciences University. Portland, OR, USA; Department of Molecular and Medical Genetics. Oregon Health and Science University. Portland, OR. USA. Electronic address: shalhub@ohsu.edu. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Vasc Surg JT - Journal of vascular surgery JID - 8407742 SB - IM OTO - NOTNLM OT - COL3A1 OT - Connective tissues disorder OT - Femoral artery OT - Genetic arteriopathy OT - Vascular Ehlers-Danlos Syndrome EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:33 PHST- 2026/06/27 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:33 [entrez] AID - S0741-5214(26)01778-7 [pii] AID - 10.1016/j.jvs.2026.10.023 [doi] PST - aheadofprint SO - J Vasc Surg. 2026 Oct 27:S0741-5214(26)01778-7. doi: 10.1016/j.jvs.2026.10.023. PMID- 42946069 OWN - NLM STAT- Publisher LR - 20261027 IS - 1097-6809 (Electronic) IS - 0741-5214 (Linking) DP - 2026 Oct 27 TI - Contemporary patterns of lower extremity revascularization for chronic limb-threatening ischemia in the United States: 2016-2022. LID - S0741-5214(26)01768-4 [pii] LID - 10.1016/j.jvs.2026.10.015 [doi] AB - BACKGROUND: Chronic limb-threatening ischemia (CLTI) is a major contributor to morbidity and mortality. Although revascularization improves limb outcomes in CLTI, contemporary practice patterns and real-world outcomes remain poorly characterized, particularly amid conflicting evidence from randomized trials regarding optimal revascularization and nonoperative management strategies. Our study utilized the National Inpatient Sample (NIS) coupled with U.S. Census data to characterize contemporary trends of incidence, revascularization approach, and in-hospital outcomes for adults hospitalized for CLTI. METHODS: We utilized the NIS (2016-2022) to generate survey-weighted national estimates of U.S. adult hospitalizations for CLTI. Age-normalized incidence rates per 100,000 person-years were calculated using population denominators from the U.S. Census Bureau. Admissions with secondary diagnoses of trauma or arterial dissection were excluded. Our outcomes evaluated the incidence of CLTI hospitalizations and rates of in-hospital revascularization type (open, endovascular, or no revascularization), major amputation, and mortality. Outcomes by revascularization strategies (open vs. endovascular) were stratified a priori by sex, frailty (Risk Analysis Index), diabetes status, and clinical presentation subgroups. Mann-Kendal trend tested monotonic temporal trends. RESULTS: An estimated 46,915 adult CLTI hospitalizations were included (mean age 69.7 years, 40.0% women, 69.5% White). Overall CLTI incidence was 7.28 per 100,000 person-years, initially declining until nadir in 2020 with reversal and steady rise thereafter. A total of 28,763 (61.3%) CLTI hospitalizations included revascularization (n=14,252 [49.5%] open and/or n=16,950 [58.9%] endovascular). The proportion of open revascularization decreased (p(trend)=0.016), while endovascular remained stable (p(trend)=0.368), and hospitalizations without revascularization increased (p(trend)=0.016) over time. Endovascular revascularization rates did rise among females, non-frail patients, and those presenting with rest pain (all p(trend)>0.05). Overall, in-hospital amputation (n=6,270 [13.4%]) and mortality (n=1,086 [2.3%]) remained stable (p(trend)>0.05). However, the proportion of patients discharged alive and without amputation increased most substantially among hospitalizations without revascularization (p(trend)=0.007). CONCLUSION: We provide contemporary analysis of U.S. hospitalizations for CLTI, demonstrating increasing rates following 2020. Overall, in-hospital rates of open revascularization declined in favor of nonoperative strategies corresponding with the greatest increase in alive discharges without amputations. Relative selection of endovascular strategies varied notably by sex, frailty, and clinical presentation. Our findings underscore the need for further investigation into optimal CLTI patient selection and coordinated integration of non-operative care, such as medical optimization, with revascularization strategies. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Machinski, Samantha N AU - Machinski SN AD - University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. Electronic address: machinskis@upmc.edu. FAU - Mosher, Emily AU - Mosher E AD - Division of Vascular Surgery, UPMC, Pittsburgh, PA, USA. FAU - Ostach, Mary Ann AU - Ostach MA AD - Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA. FAU - Shetty, Neha AU - Shetty N AD - University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. FAU - Iyer, Stuthi AU - Iyer S AD - University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. FAU - Yuo, Theodore H AU - Yuo TH AD - University of Pittsburgh School of Medicine, Pittsburgh, PA, USA; Division of Vascular Surgery, UPMC, Pittsburgh, PA, USA; Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA. FAU - Sridharan, Natalie D AU - Sridharan ND AD - University of Pittsburgh School of Medicine, Pittsburgh, PA, USA; Division of Vascular Surgery, UPMC, Pittsburgh, PA, USA; Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA. FAU - Liang, Nathan L AU - Liang NL AD - University of Pittsburgh School of Medicine, Pittsburgh, PA, USA; Division of Vascular Surgery, UPMC, Pittsburgh, PA, USA; Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA. FAU - Reitz, Katherine M AU - Reitz KM AD - University of Pittsburgh School of Medicine, Pittsburgh, PA, USA; Division of Vascular Surgery, UPMC, Pittsburgh, PA, USA; Department of Surgery, University of Pittsburgh, Pittsburgh, PA, USA; Veterans Affairs Pittsburgh Health System, Pittsburgh, PA, USA. Electronic address: ReitzKM2@UPMC.edu. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Vasc Surg JT - Journal of vascular surgery JID - 8407742 SB - IM OTO - NOTNLM OT - chronic limb threatening ischemia OT - endovascular intervention OT - lower extremity revascularization OT - peripheral arterial disease EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:33 PHST- 2026/07/22 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:33 [entrez] AID - S0741-5214(26)01768-4 [pii] AID - 10.1016/j.jvs.2026.10.015 [doi] PST - aheadofprint SO - J Vasc Surg. 2026 Oct 27:S0741-5214(26)01768-4. doi: 10.1016/j.jvs.2026.10.015. PMID- 42946064 OWN - NLM STAT- Publisher LR - 20261027 IS - 1872-7549 (Electronic) IS - 0166-4328 (Linking) DP - 2026 Oct 27 TI - Aging reshapes the response to subchronic LPS: cognitive and neuroinflammatory resilience with selective amyloid vulnerability and reduced responsiveness to L-Theanine and aerobic exercise. PG - 116553 LID - S0166-4328(26)00529-2 [pii] LID - 10.1016/j.bbr.2026.116553 [doi] AB - Aging alters the brain's inflammatory profile, which may influence vulnerability to neuroinflammatory insults and the effectiveness of neuroprotective interventions. This study investigated whether prophylactic administration of L-theanine (LT) and aerobic exercise could attenuate behavioral and molecular changes induced by subchronic systemic lipopolysaccharide (LPS) exposure in aged rats. Three- and eighteen-month-old male Wistar rats received LT, exercise, or both prior to repeated LPS administration. Behavioral assessments included working, recognition, and spatial short-term memory, followed by hippocampal analyses of inflammatory cytokines, the pCREB/CREB ratio, and Abeta(1)-(4)(2) expression. Both aging and LPS administration in 3-month-old rats impaired working memory, spatial short-term memory, and motor activity compared to young adult controls. Unlike young adult animals, subchronic LPS exposure did not impair cognitive performance in aged rats. LPS exposure resulted in reduced body weight and increased Abeta(1)-(4)(2) levels, while IL-1beta, TNF-alpha, and the pCREB/CREB ratio remained unchanged in aged rats. Prophylactic LT and/or aerobic exercise produced modest behavioral improvements, enhancing working memory in the Y-maze, but did not significantly alter hippocampal inflammatory cytokines, the pCREB/CREB ratio, or Abeta(1)-(4)(2) expression in old rats. In conclusion, aged rats exhibited relative resistance to LPS-induced cognitive and inflammatory effects but remained susceptible to amyloid-related changes. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Tchekalarova, Jana AU - Tchekalarova J AD - Institute of Neurobiology, Bulgarian Academy of Science, 1113 Sofia, Bulgaria. Electronic address: jt.chekalarova@inb.bas.bg. FAU - Hadzhipetrov, Georgi AU - Hadzhipetrov G AD - Department of Physiology, Faculty of Medicine, Medical University of Plovdiv, 4000 Plovdiv, Bulgaria. Electronic address: georgi.hadzhipetrov@mu-plovdiv.bg. FAU - Ivanova, Petja AU - Ivanova P AD - Institute of Neurobiology, Bulgarian Academy of Science, 1113 Sofia, Bulgaria. Electronic address: ivanova.petya91@gmail.com. FAU - Krushovlieva, Desislava AU - Krushovlieva D AD - Institute of Neurobiology, Bulgarian Academy of Science, 1113 Sofia, Bulgaria. Electronic address: daisyveko@gmail.com. FAU - Georgieva, Katerina AU - Georgieva K AD - Department of Physiology, Faculty of Medicine, Medical University of Plovdiv, 4000 Plovdiv, Bulgaria. Electronic address: kng@plov.net. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Behav Brain Res JT - Behavioural brain research JID - 8004872 SB - IM OTO - NOTNLM OT - Aerobic exercise OT - Cognition OT - Hippocampus OT - L-theanine OT - Lipopolysaccharide OT - beta-amyloid COIS- Declaration of Competing Interest The authors declare no conflicts of interest. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:32 PHST- 2026/08/29 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:32 [entrez] AID - S0166-4328(26)00529-2 [pii] AID - 10.1016/j.bbr.2026.116553 [doi] PST - aheadofprint SO - Behav Brain Res. 2026 Oct 27:116553. doi: 10.1016/j.bbr.2026.116553. PMID- 42946060 OWN - NLM STAT- Publisher LR - 20261027 IS - 1872-8227 (Electronic) IS - 0168-8227 (Linking) DP - 2026 Oct 27 TI - Prognostic value of stress hyperglycemia ratio formulations, random plasma glucose, and fasting plasma glucose for early prediction of acute respiratory failure requiring intubation in patients with thrombolyzed acute ischemic stroke. PG - 113629 LID - S0168-8227(26)00549-8 [pii] LID - 10.1016/j.diabres.2026.113629 [doi] AB - AIMS: The prognostic value of different stress hyperglycemia ratio (SHR) formulas for acute respiratory failure (ARF) after intravenous thrombolysis for acute ischemic stroke (AIS) remains unclear. We therefore compared four SHR formulas and examined their associations with ARF requiring invasive mechanical ventilation. METHODS: This retrospective cohort included 345 patients with AIS treated with recombinant tissue plasminogen activator (rt-PA) at Saraburi Hospital between January 2015 and July 2022. Four SHR formulas (SHR1-SHR4) were derived from fasting plasma glucose (FPG), random plasma glucose (RPG), and HbA1c. Receiver operating characteristic (ROC) analysis assessed discrimination, and multivariable logistic regression evaluated associations with ARF. RESULTS: ARF occurred in 19.4% of patients. SHR1 (FPG/HbA1c) showed the highest discrimination (AuROC = 0.740; 95% CI, 0.673-0.807; optimal cutoff, >/=1.16) and a negative predictive value of 90.4%. SHR1 >/= 1.16 was strongly associated with ARF (adjusted OR = 4.12; 95% CI, 1.99-8.53; p < 0.001) and improved model discrimination (DeltaAuROC = 0.011) and reclassification (cfNRI = 0.559; IDI = 0.023). CONCLUSIONS: SHR1 may provide incremental prognostic value for early identification of post-thrombolysis respiratory failure. Prospective multicenter validation is warranted. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Krongsut, Sarawut AU - Krongsut S AD - Division of Neurology, Department of Internal Medicine, Saraburi Hospital, Saraburi, Thailand. Electronic address: sarawut-kron@moph.go.th. FAU - Na-Ek, Nat AU - Na-Ek N AD - Division of Pharmacy Practice, Department of Pharmaceutical Care, School of Pharmaceutical Sciences, University of Phayao, Phayao, Thailand; Pharmacoepidemiology, Social and Administrative Pharmacy (P-SAP) Research Unit, School of Pharmaceutical Sciences, University of Phayao, Phayao, Thailand. Electronic address: nat.na@up.ac.th. LA - eng PT - Journal Article DEP - 20261027 PL - Ireland TA - Diabetes Res Clin Pract JT - Diabetes research and clinical practice JID - 8508335 SB - IM OTO - NOTNLM OT - Ischemic stroke OT - Prognosis OT - Respiratory insufficiency OT - Stress hyperglycemia ratio OT - Thrombolytic therapy COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:32 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:32 [entrez] AID - S0168-8227(26)00549-8 [pii] AID - 10.1016/j.diabres.2026.113629 [doi] PST - aheadofprint SO - Diabetes Res Clin Pract. 2026 Oct 27:113629. doi: 10.1016/j.diabres.2026.113629. PMID- 42946056 OWN - NLM STAT- Publisher LR - 20261027 IS - 2156-5376 (Electronic) IS - 2161-8313 (Linking) DP - 2026 Oct 27 TI - Lactose, Lactose Maldigestion and Lactose Intolerance: Definitions, Misunderstandings, Mechanisms, and Public Health Implications. PG - 100760 LID - S2161-8313(26)00174-2 [pii] LID - 10.1016/j.advnut.2026.100760 [doi] AB - Lactose is the principal carbohydrate in milk, serving a critical role in infant energy metabolism as well as contributing to neurodevelopment, and the establishment of a healthy gut microbiota. Later in life, lactose digestion capacity varies widely across human populations owing to the genetically determined decline of intestinal lactase expression after weaning-a condition termed 'lactase non-persistence'. Approximately two-thirds of the global adult population exhibits reduced lactase activity, yet the clinical consequences of this enzymatic reduction are far less uniform than commonly assumed. This review provides a comprehensive examination of lactose functionality, the physiology of lactose digestion, and the consequences of lactase persistence and non-persistence. We present formal definitions for the spectrum of terms used in lactose research-including lactase persistence, lactase non-persistence, lactose maldigestion, and lactose intolerance-and argue that the critical distinction between lactose maldigestion (a physiological state) and lactose intolerance (a clinical syndrome) is frequently overlooked in both research and public health. Drawing on evidence from double-blind randomised controlled trials , microbiome intervention studies, and global epidemiological data, the evidence indicates that the majority of individuals with lactose maldigestion do not develop clinically meaningful symptoms at moderate intake levels of most dairy products. We further discuss the mechanism of colonic adaptation, whereby gradual lactose exposure selectively enriches Bifidobacterium populations capable of fermenting lactose without excessive gas production resulting in greater lactose tolerance. Finally, we examine the public health implications of these findings, noting that food-based dietary guidelines worldwide-including those in populations with high prevalence of lactase non-persistence-consistently recommend dairy consumption owing to its established associations with reduced risk of type 2 diabetes, colorectal cancer, and improved bone health. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Inc. All rights reserved. FAU - Geurts, Jan AU - Geurts J AD - FrieslandCampina, 3818LE Amersfoort, The Netherlands. FAU - Pot, Bruno AU - Pot B AD - Yakult Europe BV, Science Department, Almere, The Netherlands. FAU - Knipping, Karen AU - Knipping K AD - Ausnutria B.V, Zwolle, the Netherlands. FAU - Braat, Mandy AU - Braat M AD - Nederlandse Zuivel Organisatie (NZO), 2596 BC The Hague, The Netherlands. FAU - Peters, Stephan AU - Peters S AD - Nederlandse Zuivel Organisatie (NZO), 2596 BC The Hague, The Netherlands. Electronic address: peters@nzo.nl. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Adv Nutr JT - Advances in nutrition (Bethesda, Md.) JID - 101540874 SB - IM OTO - NOTNLM OT - Bifidobacterium OT - colonic adaptation OT - dairy OT - food-based dietary guidelines OT - lactase non-persistence OT - lactase persistence OT - lactose OT - lactose intolerance OT - lactose maldigestion OT - lactose tolerance COIS- Conflicts of interest M.B. and S.P. are employed by the Dutch Dairy Association and B.P., K.K., J.G. are employed at companies that are members of the Dutch Dairy Association. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:32 PHST- 2026/07/30 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:32 [entrez] AID - S2161-8313(26)00174-2 [pii] AID - 10.1016/j.advnut.2026.100760 [doi] PST - aheadofprint SO - Adv Nutr. 2026 Oct 27:100760. doi: 10.1016/j.advnut.2026.100760. PMID- 42946055 OWN - NLM STAT- Publisher LR - 20261027 IS - 2468-7847 (Electronic) IS - 2468-7847 (Linking) DP - 2026 Oct 27 TI - CERVICAL CANCER: NATIONAL CLINICAL PRACTICE GUIDELINES FROM THE FRENCH CERVICAL CANCER GUIDELINES GROUP. PG - 103314 LID - S2468-7847(26)00213-8 [pii] LID - 10.1016/j.jogoh.2026.103314 [doi] AB - Over the past decade, cervical cancer management has evolved substantially, with major changes in surgical approach, surgical radicality, nodal staging, imaging, radiotherapy techniques, brachytherapy and systemic treatments. To harmonize practice and integrate new evidence into clinically applicable recommendations, the French Cervical Cancer Guidelines Group developed national clinical practice guidelines for the management of patients with cervical cancer. The guideline process was conducted under the auspices of the French National College of Obstetricians and Gynecologists and involved a multidisciplinary expert panel representing the French scientific societies involved in cervical cancer care. Clinical questions were formulated using the PICO framework and addressed the full continuum of care, including initial assessment, imaging, diagnostic conization, surgical nodal staging, treatment, fertility preservation, response assessment, follow-up, recurrence and specific clinical situations. Evidence was reviewed and critically appraised according to the GRADE methodology, and all recommendations were based on the 2018 FIGO staging system. External validation was performed through a two-round Delphi process involving 87 independent reviewers. Overall, 130 PICO questions generated 158 recommendations, including 35 strong and 52 weak recommendations, while no recommendation could be issued for 71 questions. Key practice-changing recommendations include the role of diagnostic conization in early-stage assessment, avoidance of minimally invasive hysterectomy from stage IB1 disease, risk-adapted surgical radicality, sentinel lymph node biopsy with ultrastaging, image-guided brachytherapy after chemoradiotherapy, and integration of PEMBROLIZUMAB in selected locally advanced or metastatic settings. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Masson SAS.. All rights reserved. FAU - Carcopino, Xavier AU - Carcopino X AD - Department of Obstetrics and Gynaecology, Hopital Nord, Assistance Publique-Hopitaux de Marseille (APHM), CEReSS, Center for Studies and Research on Health Services and Quality of Life, Aix Marseille University (AMU), Marseille, France. Electronic address: xavier.carcopino-tusoli@ap-hm.fr. FAU - Ouldamer, Lobna AU - Ouldamer L AD - Department of Gynecology, Centre Hospitalier Universitaire de Tours, INSERM U1069; Universite Francois-Rabelais, Tours, France. FAU - Huchon, Cyrille AU - Huchon C AD - Department of Obstetrics and Gynaecology, Lariboisiere Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Paris Cite University, Paris, France. FAU - Asseeva, Pauline AU - Asseeva P AD - Department of Nuclear Medicine, Hopital Nord, Assistance Publique-Hopitaux de Marseille (APHM), Marseille, France. FAU - Azais, Henri AU - Azais H AD - Department of Gynecologic and Breast Cancer Surgery, Hopital Europeen Georges Pompidou, AP-HP, Paris Cite University, Paris, France. FAU - Bendifallah, Sofiane AU - Bendifallah S AD - Department of Gynaecologic Surgery, American Hospital of Paris, Neuilly-sur-Seine, France. FAU - Brun, Jean-Luc AU - Brun JL AD - Department of Gynaecologic Surgery and Reproductive Medicine, Alienor d'Aquitaine Center, Pellegrin Hospital, Bordeaux, France. FAU - Canlorbe, Geoffroy AU - Canlorbe G AD - Department of Gynaecologic and Breast Cancer Surgery, Pitie-Salpetriere Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Saint-Antoine Research Center (CRSA), INSERM UMR_S_938, Cancer Biology and Therapeutics, Paris, France. FAU - Chargari, Cyrus AU - Chargari C AD - Department of Radiation Oncology, Pitie-Salpetriere Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Paris, France. FAU - Chauvet, Pauline AU - Chauvet P AD - Department of Obstetrics and Gynaecology, Estaing University Hospital, Clermont Auvergne University, EnCoV, Institut Pascal, CNRS UMR 6602, SIGMA Clermont, Clermont-Ferrand, France. FAU - Cordoba, Abel AU - Cordoba A AD - Department of Radiation Therapy, Centre Oscar Lambret, Lille, France. FAU - Courcier, Helene AU - Courcier H AD - Department of Obstetrics and Gynaecology, Bichat Hospital, Paris Cite University, Paris, France. FAU - Dabi, Yohann AU - Dabi Y AD - Department of Obstetrics and Gynaecology and Reproductive Medicine, Tenon Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Paris, France. FAU - de Berti, Marion AU - de Berti M AD - Department of Gynecology, Centre Hospitalier Universitaire de Tours, INSERM U1069; Universite Francois-Rabelais, Tours, France. FAU - Diguisto, Caroline AU - Diguisto C AD - Department of Gynecology, Centre Hospitalier Universitaire de Tours, INSERM U1069; Universite Francois-Rabelais, Tours, France. FAU - Fuss, Eva AU - Fuss E AD - Department of Gynaecologic Surgery, Hautepierre University Hospital, Strasbourg, France. FAU - Gaillard, Thomas AU - Gaillard T AD - Department of Gynaecologic, Breast and Reconstructive Surgery, Institut Curie, Paris, France. FAU - Gauthier, Tristan AU - Gauthier T AD - Department of Obstetrics and Gynaecology, Women, Mothers and Children Hospital (HFME), Limoges University Hospital, Limoges, France. FAU - Gertych, Witold AU - Gertych W AD - Department of Gynaecologic and Oncologic Surgery, Lyon Sud Hospital, Lyon, France. FAU - Gladieff, Laurence AU - Gladieff L AD - Department of Medical Oncology, Oncopole Claudius Regaud, IUCT-Oncopole, Toulouse, France. FAU - Graesslin, Olivier AU - Graesslin O AD - Alix de Champagne Mother and Child Institute, Reims University Hospital, University of Reims Champagne-Ardenne (URCA), Reims, France. FAU - Kerbage, Yohan AU - Kerbage Y AD - Department of Gynaecologic Surgery, Lille University Hospital, University of Lille, INSERM U1189, OncoThAI: Laser-Assisted Therapies and Immunotherapies for Oncology, Lille, France. FAU - Koskas, Martin AU - Koskas M AD - Department of Obstetrics and Gynaecology, Bichat Hospital, Paris Cite University, Paris, France. FAU - Laas, Enora AU - Laas E AD - Department of Gynaecologic, Breast and Reconstructive Surgery, Institut Curie, Paris, France. FAU - Lacorre, Aymeline AU - Lacorre A AD - Department of Obstetrics and Gynaecology, Women, Mothers and Children Hospital (HFME), Limoges University Hospital, Limoges, France. FAU - Lavoue, Vincent AU - Lavoue V AD - Department of Gynaecology, Obstetrics and Human Reproduction, CHU Anne de Bretagne, Faculty of Medicine, University of Rennes, IRSET, INSERM U1085, Rennes, France. FAU - Lecointre, Lise AU - Lecointre L AD - Department of Gynaecologic Surgery, Hautepierre University Hospital, Strasbourg, France. FAU - Lecuru, Fabrice AU - Lecuru F AD - Department of Breast, Gynaecologic and Reconstructive Surgery, Institut Curie, Faculty of Medicine, Paris Cite University, Paris, France. FAU - Margueritte, Francois AU - Margueritte F AD - Department of Obstetrics and Gynaecology, Women, Mothers and Children Hospital (HFME), Limoges University Hospital, Limoges, France. FAU - Martinez, Alejandra AU - Martinez A AD - Oncopole Claudius Regaud, Toulouse University Cancer Institute-Oncopole, Toulouse III-Paul Sabatier University, INSERM, CNRS, University of Toulouse, Toulouse Cancer Research Center, UMR1037, Toulouse, France. FAU - Maternowski, Paul-Jean AU - Maternowski PJ AD - Department of Gynaecologic Surgery and Reproductive Medicine, Alienor d'Aquitaine Center, Pellegrin Hospital, Bordeaux, France. FAU - Mathevet, Patrice AU - Mathevet P AD - Lausanne University Hospital (CHUV), Lausanne, Switzerland; University of Lausanne (UNIL), Lausanne, Switzerland. FAU - Menouer, Imane AU - Menouer I AD - Alix de Champagne Mother and Child Institute, Reims University Hospital, University of Reims Champagne-Ardenne (URCA), Reims, France. FAU - Mimoun, Camille AU - Mimoun C AD - Department of Obstetrics and Gynaecology, Lariboisiere Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Paris Cite University, Paris, France. FAU - Moureau, Anthony AU - Moureau A AD - Department of Gynaecology, Obstetrics and Human Reproduction, CHU Anne de Bretagne, Faculty of Medicine, University of Rennes, IRSET, INSERM U1085, Rennes, France. FAU - Netter, Antoine AU - Netter A AD - Department of Obstetrics and Gynaecology, Hopital Nord, Assistance Publique-Hopitaux de Marseille (APHM), CEReSS, Center for Studies and Research on Health Services and Quality of Life, Aix Marseille University (AMU), Marseille, France. FAU - Philip, Charles-Andre AU - Philip CA AD - Women and Children Hospital, Hospices Civils de Lyon (HCL), LabTAU INSERM U1032, Claude Bernard Lyon 1 University, Lyon, France. FAU - Rousset, Pascal AU - Rousset P AD - Department of Medical and Interventional Imaging, Lyon Sud University Hospital, Hospices Civils de Lyon, Claude Bernard Lyon 1 University, EA CICLY, Lyon, France. FAU - Sanson, Claire AU - Sanson C AD - Department of Gynaecologic Surgery, Paris Saint-Joseph Hospital Group, Paris, France. FAU - Thomassin-Naggara, Isabelle AU - Thomassin-Naggara I AD - Department of Medical Imaging, Tenon Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Paris, France. FAU - Touboul, Cyril AU - Touboul C AD - Department of Obstetrics and Gynaecology and Reproductive Medicine, Tenon Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Paris, France. FAU - Leveque, Jean AU - Leveque J AD - Department of Gynaecology, Obstetrics and Human Reproduction, CHU Anne de Bretagne, Faculty of Medicine, University of Rennes, IRSET, INSERM U1085, Rennes, France. LA - eng PT - Journal Article PT - Practice Guideline DEP - 20261027 PL - France TA - J Gynecol Obstet Hum Reprod JT - Journal of gynecology obstetrics and human reproduction JID - 101701588 SB - IM OTO - NOTNLM OT - Cervical cancer OT - Chemoradiotherapy OT - Clinical practice guidelines OT - Staging OT - Surgery COIS- Declaration of competing interest National clinical practice guidelines for cervical cancer This declaration summarizes the competing interests of the 42 authors of the French national clinical practice guidelines for cervical cancer. Competing interests Martin Koskas reported a consulting or advisory relationship with Intuitive Surgical Inc. Geoffroy Canlorbe reported a consulting or advisory relationship with Intuitive Surgical Inc. Vincent Lavoue reported a consulting or advisory relationship with Intuitive Surgical Inc. No competing interests reported Xavier Carcopino, Lobna Ouldamer, Cyrille Huchon, Pauline Asseeva, Henri Azais, Sofiane Bendifallah, Jean-Luc Brun, Cyrus Chargari, Pauline Chauvet, Abel Cordoba, Helene Courcier, Yohann Dabi, Marion de Berti, Caroline Diguisto, Eva Fuss, Thomas Gaillard, Tristan Gauthier, Witold Gertych, Laurence Gladieff, Olivier Graesslin, Yohan Kerbage, Enora Laas, Aymeline Lacorre, Lise Lecointre, Fabrice Lecuru, Francois Margueritte, Alejandra Martinez, Paul-Jean Maternowski, Patrice Mathevet, Imane Menouer, Camille Mimoun, Anthony Moureau, Antoine Netter, Charles-Andre Philip, Pascal Rousset, Claire Sanson, Isabelle Thomassin-Naggara, Cyril Touboul, Jean Leveque EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:32 PHST- 2026/08/23 00:00 [received] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:32 [entrez] AID - S2468-7847(26)00213-8 [pii] AID - 10.1016/j.jogoh.2026.103314 [doi] PST - aheadofprint SO - J Gynecol Obstet Hum Reprod. 2026 Oct 27:103314. doi: 10.1016/j.jogoh.2026.103314. PMID- 42946054 OWN - NLM STAT- Publisher LR - 20261027 IS - 2468-7847 (Electronic) IS - 2468-7847 (Linking) DP - 2026 Oct 27 TI - Risk-Adapted surgical treatment of early-stage cervical cancer: tailoring radicality, ovarian preservation, and surgical approach in the post-shape and post-lacc era - A review from the french cervical cancer guidelines group. PG - 103316 LID - S2468-7847(26)00215-1 [pii] LID - 10.1016/j.jogoh.2026.103316 [doi] AB - BACKGROUND: Exclusive surgical treatment remains a cornerstone of management for selected patients with early-stage cervical cancer. However, the traditional paradigm of radical hysterectomy with systematic parametrial resection has been challenged by contemporary evidence supporting risk-adapted surgical de-escalation. In parallel, randomized and real-world data have raised major concerns regarding the oncologic safety of minimally invasive radical hysterectomy. METHODS: A structured narrative review was conducted to summarize the available evidence on exclusive surgical treatment for early-stage cervical cancer. Predefined topics included the extent of hysterectomy and parametrial resection, ovarian preservation, surgical approach, and the comparative role of surgery versus chemoradiotherapy. Randomized trials, prospective studies, retrospective cohorts, meta-analyses, and relevant methodological reviews were included. RESULTS: In early-stage cervical cancer, parametrial involvement is uncommon in well-selected low-risk patients. The SHAPE trial demonstrated the non-inferiority of simple hysterectomy compared with radical hysterectomy in low-risk disease, with lower urinary morbidity, supporting risk-adapted omission of parametrial resection. Ovarian preservation appears oncologically safe in young women with early-stage disease and no high-risk features, although caution is warranted in the presence of adverse factors such as uterine corpus involvement, lymphovascular space invasion, lymph node metastases, and non-squamous histology. Conversely, minimally invasive radical hysterectomy has been associated with inferior disease-free and overall survival compared with open surgery, despite reduced perioperative morbidity. CONCLUSION: Exclusive surgical treatment for early-stage cervical cancer should be guided by individualized risk assessment, combining parametrial de-escalation in low-risk disease, ovarian preservation when appropriate, and open surgery when radical hysterectomy is required. CI - Copyright (c) 2026. Published by Elsevier Masson SAS. FAU - Ouldamer, Lobna AU - Ouldamer L AD - Department of Gynecology, Centre Hospitalier Universitaire de Tours, INSERM U1069; Universite Francois-Rabelais, Tours, France,. Electronic address: louldamer@yahoo.fr. FAU - Deberti, Marion AU - Deberti M AD - Department of Gynecology, Centre Hospitalier Universitaire de Tours, INSERM U1069; Universite Francois-Rabelais, Tours, France. FAU - Morineau, Faustine AU - Morineau F AD - Hospices Civils de Lyon, Lyon Sud Hospital, Department of Gynecological Surgery and Obstetrics, Lyon, France. FAU - Carcopino, Xavier AU - Carcopino X AD - Department of Obstetrics and Gynaecology, Hopital Nord, Assistance Publique-Hopitaux de Marseille (APHM), CEReSS, Center for Studies and Research on Health Services and Quality of Life, Aix Marseille University (AMU), Marseille, France. FAU - Gertych, Witold AU - Gertych W AD - Hospices Civils de Lyon, Lyon Sud Hospital, Department of Gynecological Surgery and Obstetrics, Lyon, France. CN - members from the FRENCH CERVICAL CANCER GUIDELINES GROUP LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - France TA - J Gynecol Obstet Hum Reprod JT - Journal of gynecology obstetrics and human reproduction JID - 101701588 SB - IM OTO - NOTNLM OT - Cervical cancer OT - Early stage OT - Hysterectomy OT - Minimally invasive surgery OT - Radical hysterectomy COIS- Declaration of competing interest The authors declare that they have no conflict of interest EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:32 PHST- 2026/06/15 00:00 [received] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:32 [entrez] AID - S2468-7847(26)00215-1 [pii] AID - 10.1016/j.jogoh.2026.103316 [doi] PST - aheadofprint SO - J Gynecol Obstet Hum Reprod. 2026 Oct 27:103316. doi: 10.1016/j.jogoh.2026.103316. PMID- 42946053 OWN - NLM STAT- Publisher LR - 20261027 IS - 2468-7847 (Electronic) IS - 2468-7847 (Linking) DP - 2026 Oct 27 TI - Special situations in cervical cancer management: de novo metastatic disease, occult cancer after simple hysterectomy, and cervical stump cancer - A review from the french cervical cancer guidelines group. PG - 103312 LID - S2468-7847(26)00211-4 [pii] LID - 10.1016/j.jogoh.2026.103312 [doi] AB - INTRODUCTION: Cervical cancer may present in uncommon and complex clinical settings, including de novo metastatic disease, occult cancer incidentally diagnosed after simple hysterectomy and cervical stump cancer after supracervical hysterectomy. METHODS: A literature review was conducted using PubMed/MEDLINE, the Cochrane Library and Scopus. English- and French-language publications available through September 2025 were qualitatively analyzed according to their methodological quality, level of evidence and clinical relevance. RESULTS: In de novo metastatic cervical cancer, platinum-based chemotherapy remains the backbone of systemic treatment. BEVACIZUMAB and PEMBROLIZUMAB have improved outcomes in trials combining de novo metastatic, persistent and recurrent disease, although their specific benefit in the de novo subgroup remains less clearly established. Local treatment of oligometastases has been associated with prolonged survival in selected patients, but controlled comparative evidence is lacking. For occult cervical cancer after simple hysterectomy, favorable outcomes without additional treatment have been reported in selected patients with small tumors, limited stromal invasion, negative margins and no nodal involvement. Completion surgery and radiotherapy-based treatments have achieved disease control in patients with more extensive disease or adverse pathological features, but their comparative efficacy remains uncertain. In cervical stump cancer, historical series suggest that external beam radiotherapy combined with brachytherapy provides effective locoregional control. Evidence regarding contemporary image-guided brachytherapy is limited, while completion surgery after radiotherapy has been associated with substantial morbidity without demonstrated improvement in disease control. CONCLUSION: The available evidence remains limited by heterogeneous populations, historical staging and treatment techniques, selection bias, and the absence of prospective controlled comparisons. CI - Copyright (c) 2026. Published by Elsevier Masson SAS. FAU - Fuss, Eva AU - Fuss E AD - Department of Gynecologic Surgery, Hautepierre University Hospital, Strasbourg, France. FAU - Lecointre, Lise AU - Lecointre L AD - Department of Gynecologic Surgery, Hautepierre University Hospital, Strasbourg, France. Electronic address: lise.lecointre@chru-strasbourg.fr. FAU - Gladieff, Laurence AU - Gladieff L AD - Department of Medical Oncology, Oncopole Claudius Regaud, IUCT-Oncopole, Toulouse, France. FAU - Bendifallah, Sofiane AU - Bendifallah S AD - Department of Gynecologic Surgery, American Hospital of Paris, Neuilly-sur-Seine, France. FAU - Menouer, Imane AU - Menouer I AD - Alix de Champagne Mother and Child Institute, Reims University Hospital, University of Reims Champagne-Ardenne (URCA), Reims, France. FAU - Courcier, Helene AU - Courcier H AD - Department of Obstetrics and Gynecology, Bichat Hospital, Paris Cite University, Paris, France. FAU - Koskas, Martin AU - Koskas M AD - Department of Obstetrics and Gynecology, Bichat Hospital, Paris Cite University, Paris, France. FAU - Carcopino, Xavier AU - Carcopino X AD - Department of Obstetrics and Gynecology, North Hospital, Assistance Publique-Hopitaux de Marseille (APHM), CEReSS, Center for Studies and Research on Health Services and Quality of Life, Aix Marseille University (AMU), Marseille, France. CN - members from the FRENCH CERVICAL CANCER GUIDELINES GROUP LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - France TA - J Gynecol Obstet Hum Reprod JT - Journal of gynecology obstetrics and human reproduction JID - 101701588 SB - IM OTO - NOTNLM OT - Cervical cancer OT - anti-angiogenic OT - concomitant chemoradiotherapy OT - image-guided brachytherapy OT - immunotherapy OT - metastatic cancer COIS- Declaration of competing interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:32 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:32 [entrez] AID - S2468-7847(26)00211-4 [pii] AID - 10.1016/j.jogoh.2026.103312 [doi] PST - aheadofprint SO - J Gynecol Obstet Hum Reprod. 2026 Oct 27:103312. doi: 10.1016/j.jogoh.2026.103312. PMID- 42946052 OWN - NLM STAT- Publisher LR - 20261027 IS - 2468-7847 (Electronic) IS - 2468-7847 (Linking) DP - 2026 Oct 27 TI - FERTILITY-SPARING SURGERY AND OVARIAN TRANSPOSITION IN CERVICAL CANCER - A REVIEW FROM THE FRENCH CERVICAL CANCER GUIDELINES GROUP. PG - 103313 LID - S2468-7847(26)00212-6 [pii] LID - 10.1016/j.jogoh.2026.103313 [doi] AB - Cervical cancer often affects women of reproductive age, for whom treatment may compromise both fertility and ovarian endocrine function. Fertility-sparing surgery may preserve the ability to achieve pregnancy, whereas Ovarian Preservation (OP) during hysterectomy and Ovarian Transposition (OT) before pelvic radiotherapy may prevent treatment-induced premature ovarian insufficiency. However, the oncological safety and appropriate indications for these strategies require careful assessment. Despite the low certainty of evidence, conization with negative margins may be offered for FIGO 2018 stage IA1-IA2 disease, irrespective of lymphovascular space invasion (LVSI). Simple trachelectomy may be preferred for stage IB1 disease without LVSI, whereas evidence remains insufficient to choose between simple and radical trachelectomy in LVSI-positive disease. Routine cerclage at the time of trachelectomy is not supported by current evidence. Completion hysterectomy after childbearing provides no established oncological benefit and should not be systematically performed. For stage IB2 disease, neoadjuvant chemotherapy followed by fertility-sparing surgery remains investigational because of uncertain oncological safety and a potential adverse effect on survival. OP appears oncologically acceptable in carefully selected premenopausal patients undergoing surgery, particularly those with early-stage squamous cell carcinoma. OT preserves ovarian function in approximately two-thirds of patients receiving pelvic radiotherapy, especially in women younger than 40 years when both ovaries are positioned above the radiation field. However, OP and OT must be balanced against the risk of ovarian metastasis and should be approached cautiously in patients with adenocarcinoma, advanced disease, or uterine corpus, parametrial, or lymph node involvement. CI - Copyright (c) 2026. Published by Elsevier Masson SAS. FAU - Margueritte, Francois AU - Margueritte F AD - Department of Obstetrics and Gynaecology, Women, Mothers and Children Hospital (HFME), Limoges University Hospital, Limoges, France; Universite Paris-Saclay, UVSQ, INSERM, CESP, Team << Primary care and Prevention >>, 94807, Villejuif, France. Electronic address: fmargueritte@gmail.com. FAU - Mathevet, Patrice AU - Mathevet P AD - Lausanne University Hospital (CHUV), Lausanne, Switzerland; University of Lausanne (UNIL), Lausanne, Switzerland. FAU - Diguisto, Caroline AU - Diguisto C AD - Department of Obstetrics, University of Tours Faculty of Medicine, Tours, France. FAU - Carcopino, Xavier AU - Carcopino X AD - Department of Obstetrics and Gynaecology, Hopital Nord, Assistance Publique-Hopitaux de Marseille (APHM), CEReSS, Center for Studies and Research on Health Services and Quality of Life, Aix Marseille University (AMU), Marseille, France. FAU - Phillip, Charles-Andre AU - Phillip CA AD - Women and Children Hospital, Hospices Civils de Lyon (HCL), LabTAU INSERM U1032, Claude Bernard Lyon 1 University, Lyon, France. CN - members from the FRENCH CERVICAL CANCER GUIDELINES GROUP FAU - Carcopino, Xavier AU - Carcopino X AD - Department of Obstetrics and Gynaecology., Hopital Nord, Assistance Publique-Hopitaux de Marseille (APHM), CEReSS, Center for Studies and Research on Health Services and Quality of Life, Aix Marseille University (AMU), Marseille, France. FAU - Ouldamer, Lobna AU - Ouldamer L AD - Department of Gynecology, Centre Hospitalier Universitaire de Tours, INSERM U1069; Universite Francois-Rabelais, Tours, France. FAU - Huchon, Cyrille AU - Huchon C AD - Department of Obstetrics and Gynaecology, Lariboisiere Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Paris Cite University, Paris, France. FAU - Asseeva, Pauline AU - Asseeva P AD - Department of Nuclear Medicine, Hopital Nord, Assistance Publique-Hopitaux de Marseille (APHM), Marseille, France. FAU - Azais, Henri AU - Azais H AD - Department of Gynecologic and Breast Cancer Surgery, Hopital Europeen Georges Pompidou, AP-HP, Paris Cite University, Paris, France. FAU - Bendifallah, Sofiane AU - Bendifallah S AD - Department of Gynaecologic Surgery, American Hospital of Paris, Neuilly-sur-Seine, France. FAU - Brun, Jean-Luc AU - Brun JL AD - Department of Gynaecologic Surgery and Reproductive Medicine, Alienor d'Aquitaine Center, Pellegrin Hospital, Bordeaux, France. FAU - Canlorbe, Geoffroy AU - Canlorbe G AD - Department of Gynaecologic and Breast Cancer Surgery, Pitie-Salpetriere Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Saint-Antoine Research Center (CRSA), INSERM UMR_S_938, Cancer Biology and Therapeutics, Paris, France. FAU - Chargari, Cyrus AU - Chargari C AD - Department of Radiation Oncology, Pitie-Salpetriere Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Paris, France. FAU - Chauvet, Pauline AU - Chauvet P AD - Department of Obstetrics and Gynaecology, Estaing University Hospital, Clermont Auvergne University, EnCoV, Institut Pascal, CNRS UMR 6602, SIGMA Clermont, Clermont-Ferrand, France. FAU - Cordoba, Abel AU - Cordoba A AD - Department of Radiation Therapy, Centre Oscar Lambret, Lille, France. FAU - Courcier, Helene AU - Courcier H AD - Department of Obstetrics and Gynaecology, Bichat Hospital, Paris Cite University, Paris, France. FAU - Dabi, Yohann AU - Dabi Y AD - Department of Obstetrics and Gynaecology and Reproductive Medicine, Tenon Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Paris, France. FAU - de Berti, Marion AU - de Berti M AD - Department of Gynecology, Centre Hospitalier Universitaire de Tours, INSERM U1069; Universite Francois-Rabelais, Tours, France. FAU - Diguisto, Caroline AU - Diguisto C AD - Department of Gynecology, Centre Hospitalier Universitaire de Tours, INSERM U1069; Universite Francois-Rabelais, Tours, France. FAU - Fuss, Eva AU - Fuss E AD - Department of Gynaecologic Surgery, Hautepierre University Hospital, Strasbourg, France. FAU - Gaillard, Thomas AU - Gaillard T AD - Department of Gynaecologic, Breast and Reconstructive Surgery, Institut Curie, Paris, France. FAU - Gauthier, Tristan AU - Gauthier T AD - Department of Obstetrics and Gynaecology., Women, Mothers and Children Hospital (HFME), Limoges University Hospital, Limoges, France. FAU - Gertych, Witold AU - Gertych W AD - Department of Gynaecologic and Oncologic Surgery, Lyon Sud Hospital, Lyon, France. FAU - Gladieff, Laurence AU - Gladieff L AD - Department of Medical Oncology, Oncopole Claudius Regaud, IUCT-Oncopole, Toulouse, France. FAU - Graesslin, Olivier AU - Graesslin O AD - Alix de Champagne Mother and Child Institute, Reims University Hospital, University of Reims Champagne-Ardenne (URCA), Reims, France. FAU - Kerbage, Yohan AU - Kerbage Y AD - Department of Gynaecologic Surgery, Lille University Hospital, University of Lille, INSERM U1189, OncoThAI: Laser-Assisted Therapies and Immunotherapies for Oncology, Lille, France. FAU - Koskas, Martin AU - Koskas M AD - Department of Obstetrics and Gynaecology, Bichat Hospital, Paris Cite University, Paris, France. FAU - Laas, Enora AU - Laas E AD - Department of Gynaecologic, Breast and Reconstructive Surgery, Institut Curie, Paris, France. FAU - Lacorre, Aymeline AU - Lacorre A AD - Department of Obstetrics and Gynaecology., Women, Mothers and Children Hospital (HFME), Limoges University Hospital, Limoges, France. FAU - Lavoue, Vincent AU - Lavoue V AD - Department of Gynaecology, Obstetrics and Human Reproduction, CHU Anne de Bretagne, Faculty of Medicine, University of Rennes, IRSET, INSERM U1085, Rennes, France. FAU - Lecointre, Lise AU - Lecointre L AD - Department of Gynaecologic Surgery, Hautepierre University Hospital, Strasbourg, France. FAU - Lecuru, Fabrice AU - Lecuru F AD - Department of Breast, Gynaecologic and Reconstructive Surgery, Institut Curie, Faculty of Medicine, Paris Cite University, Paris, France. FAU - Margueritte, Francois AU - Margueritte F AD - Department of Obstetrics and Gynaecology., Women, Mothers and Children Hospital (HFME), Limoges University Hospital, Limoges, France. FAU - Martinez, Alejandra AU - Martinez A AD - Oncopole Claudius Regaud, Toulouse University Cancer Institute-Oncopole, Toulouse III-Paul Sabatier University, INSERM, CNRS, University of Toulouse, Toulouse Cancer Research Center, UMR1037, Toulouse, France. FAU - Maternowski, Paul-Jean AU - Maternowski PJ AD - Department of Gynaecologic Surgery and Reproductive Medicine, Alienor d'Aquitaine Center, Pellegrin Hospital, Bordeaux, France. FAU - Mathevet, Patrice AU - Mathevet P AD - Lausanne University Hospital (CHUV)., Lausanne, Switzerland; University of Lausanne (UNIL)., Lausanne, Switzerland. FAU - Menouer, Imane AU - Menouer I AD - Alix de Champagne Mother and Child Institute, Reims University Hospital, University of Reims Champagne-Ardenne (URCA), Reims, France. FAU - Mimoun, Camille AU - Mimoun C AD - Department of Obstetrics and Gynaecology, Lariboisiere Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Paris Cite University, Paris, France. FAU - Moureau, Anthony AU - Moureau A AD - Department of Gynaecology, Obstetrics and Human Reproduction, CHU Anne de Bretagne, Faculty of Medicine, University of Rennes, IRSET, INSERM U1085, Rennes, France. FAU - Netter, Antoine AU - Netter A AD - Department of Obstetrics and Gynaecology., Hopital Nord, Assistance Publique-Hopitaux de Marseille (APHM), CEReSS, Center for Studies and Research on Health Services and Quality of Life, Aix Marseille University (AMU), Marseille, France. FAU - Philip, Charles-Andre AU - Philip CA AD - Women and Children Hospital., Hospices Civils de Lyon (HCL), LabTAU INSERM U1032, Claude Bernard Lyon 1 University, Lyon, France. FAU - Rousset, Pascal AU - Rousset P AD - Department of Medical and Interventional Imaging, Lyon Sud University Hospital, Hospices Civils de Lyon, Claude Bernard Lyon 1 University, EA CICLY, Lyon, France. FAU - Sanson, Claire AU - Sanson C AD - Department of Gynaecologic Surgery, Paris Saint-Joseph Hospital Group, Paris, France. FAU - Thomassin-Naggara, Isabelle AU - Thomassin-Naggara I AD - Department of Medical Imaging, Tenon Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Paris, France. FAU - Touboul, Cyril AU - Touboul C AD - Department of Obstetrics and Gynaecology and Reproductive Medicine, Tenon Hospital, Assistance Publique-Hopitaux de Paris (AP-HP), Sorbonne University, Paris, France. FAU - Leveque, Jean AU - Leveque J AD - Department of Gynaecology, Obstetrics and Human Reproduction, CHU Anne de Bretagne, Faculty of Medicine, University of Rennes, IRSET, INSERM U1085, Rennes, France. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - France TA - J Gynecol Obstet Hum Reprod JT - Journal of gynecology obstetrics and human reproduction JID - 101701588 SB - IM OTO - NOTNLM OT - cervix cancer OT - conization OT - fertility OT - ovarian preservation OT - ovarian transposition OT - survival OT - trachelectomy EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:32 PHST- 2026/08/26 00:00 [received] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:32 [entrez] AID - S2468-7847(26)00212-6 [pii] AID - 10.1016/j.jogoh.2026.103313 [doi] PST - aheadofprint SO - J Gynecol Obstet Hum Reprod. 2026 Oct 27:103313. doi: 10.1016/j.jogoh.2026.103313. PMID- 42946044 OWN - NLM STAT- Publisher LR - 20261027 IS - 1876-4754 (Electronic) IS - 1876-4754 (Linking) DP - 2026 Oct 27 TI - After the pulse: why post-stimulation task demands matter for detecting neural effects of non-invasive brain stimulation. PG - 103244 LID - S1935-861X(26)00221-4 [pii] LID - 10.1016/j.brs.2026.103244 [doi] FAU - Int-Veen, Isabell AU - Int-Veen I AD - Department of Psychiatry and Psychotherapy, Tubingen Center for Mental Health (TuCMH), University of Tubingen, Tubingen, Germany; German Center for Mental Health (DZPG), partner site Tubingen, Germany. FAU - Ehlis, Ann-Christine AU - Ehlis AC AD - Department of Psychiatry and Psychotherapy, Tubingen Center for Mental Health (TuCMH), University of Tubingen, Tubingen, Germany; German Center for Mental Health (DZPG), partner site Tubingen, Germany; LEAD Graduate School & Research Network, University of Tuebingen, Tuebingen, Germany. LA - eng PT - Letter DEP - 20261027 PL - United States TA - Brain Stimul JT - Brain stimulation JID - 101465726 SB - IM COIS- Declaration of Competing Interest ☒ The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Ann-Christine Ehlis reports financial support was provided by German Research Foundation (project number EH 388/2-1). If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:32 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/22 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:32 [entrez] AID - S1935-861X(26)00221-4 [pii] AID - 10.1016/j.brs.2026.103244 [doi] PST - aheadofprint SO - Brain Stimul. 2026 Oct 27:103244. doi: 10.1016/j.brs.2026.103244. PMID- 42946042 OWN - NLM STAT- Publisher LR - 20261027 IS - 1878-3554 (Electronic) IS - 0099-2399 (Linking) DP - 2026 Oct 27 TI - Effects of Irrigation Protocols on Dentin-Root Interface Integrity and Sealer Penetration after Minimally Invasive versus Conventional Instrumentation: An Ex Vivo Analysis. LID - S0099-2399(26)00535-2 [pii] LID - 10.1016/j.joen.2026.10.009 [doi] AB - INTRODUCTION: This study compared the effects of ultrasonic activation (UA), laser-activated irrigation (LAI), and the GentleWave(R) system (GW) on root dentin and sealer penetration (SP) after minimally invasive (MI) and conventional (CT) instrumentation techniques. METHODS: One hundred extracted human mandibular premolars were allocated according to the instrumentation technique. Forty teeth (n=20) were analyzed for root dentin morphology using scanning electron microscopy (SEM), and 60 teeth (n=30) were assessed for SP using confocal laser scanning microscopy (CLSM). For SEM analysis, specimens were further subdivided by irrigation protocol, including a negative control group (n=5). For SP evaluation, teeth were subdivided by irrigation protocol (n=10) and obturated with EndoSequence(R) BC Sealer labeled with 0.003% Fluo-3. Data were analyzed using the Kruskal-Wallis test and Dunn's post hoc test (p < 0.05). RESULTS: No significant differences in smear layer (SL) scores were found between CT and MI when the same irrigation technique was compared. In CT-prepared teeth, the control group exhibited higher SL than the irrigation groups, except in the middle third, where the results were comparable to those of UA. In MI-prepared teeth, GW showed lower SL scores than LAI in the cervical third (p < 0.05). In the apical third of MI-prepared canals, GW and LAI were associated with greater sealer penetration area and maximum depth than UA (p < 0.05). Dentin erosion was observed in 15 of 120 (12.5%) imageswith 10 images from the UA group and 5 from the LAI group. No erosion was observed in the GW group. CONCLUSIONS: Under the experimental conditions evaluated, no morphological signs of dentin erosion were observed with the GW protocol, and GW and LAI were associated with greater apical SP than UA following MI instrumentation. Because the protocols differed in irrigant volume, exposure time, and sequence, these findings cannot be attributed specifically to the activation technologies. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Scardini, Iandara L AU - Scardini IL AD - Department of Operative Dentistry, Faculty of Dentistry, University of Sao Paulo. Electronic address: iandara.scardini@gmail.com. FAU - Masiero, Anelise V AU - Masiero AV AD - Department of Periodontology, Endodontics and Dental Hygiene, Adams School of Dentistry, The University of North Carolina at Chapel Hill. Electronic address: avmasiero@gmail.com. FAU - Dos Santos, Marcelo AU - Dos Santos M AD - Department of Operative Dentistry, Faculty of Dentistry, University of Sao Paulo. Electronic address: msantos@usp.br. FAU - Parolia, Abhishek AU - Parolia A AD - Department of Endodontics, College of Dentistry and Dental Clinics, University of Iowa. Electronic address: abhishek-parolia@uiowa.edu. FAU - Teixeira, Fabricio B AU - Teixeira FB AD - Department of Endodontics, School of Dentistry, Texas A&M University. Electronic address: fabricio-teixeira@tamu.edu. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Endod JT - Journal of endodontics JID - 7511484 SB - IM OTO - NOTNLM OT - GentleWave OT - laser irrigation OT - sealer penetration OT - ultrasonic activation EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:32 PHST- 2025/09/08 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:32 [entrez] AID - S0099-2399(26)00535-2 [pii] AID - 10.1016/j.joen.2026.10.009 [doi] PST - aheadofprint SO - J Endod. 2026 Oct 27:S0099-2399(26)00535-2. doi: 10.1016/j.joen.2026.10.009. PMID- 42946038 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-6351 (Electronic) IS - 0278-6915 (Linking) DP - 2026 Oct 27 TI - Applications of Machine Learning in Assessing Human Dietary Exposure to Environmental Chemical Contaminants. PG - 116471 LID - S0278-6915(26)00546-6 [pii] LID - 10.1016/j.fct.2026.116471 [doi] AB - Diet is a major pathway for human exposure to environmental contaminants. Researchers have established associations between dietary intake and increased body burden of environmental chemicals. Traditional human dietary exposure assessment has limitations in accuracy, high-throughput, predictive ability, and ability to explore complex interrelations. With recent advances in artificial intelligence, this narrative review examined the applications of machine learning (ML) in human dietary exposure assessment. We conducted a search using terms/synonyms related to machine learning, environmental chemicals, diet, and exposure. Study criteria were established, and two reviewers conducted screening from February-April 2025. Nineteen (19) studies published from 2019-2025 were included. Key applications identified were detecting/quantifying contaminants in food, predicting contaminant levels in the body, developing exposure profiles, and assessing health risks from interactions among diet, contaminants, and epidemiological factors. Studies utilizing ML demonstrated improved accuracy and predictive ability compared to traditional approaches. They also provided faster, more cost-effective, and less invasive animal testing for food safety. Challenges identified include poor model performance in certain instances, limited independent external validation, limited applicability, and limited complete datasets. Our review highlights ML as a valuable tool for human dietary exposure assessment and provides key future directions in this field. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Davour, Roselyn Delase AU - Davour RD AD - Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, 2197 Mowry RD, Gainesville, FL 32611, United States. Electronic address: rdavour@ufl.edu. FAU - Nketsiah, Esther AU - Nketsiah E AD - Department of Health Sciences, College of Health Professions and Sciences, University of Central Florida, 4364 Scorpius Street, Orlando, FL 32816, United States. FAU - Colon Burgos, Jose Felix AU - Colon Burgos JF AD - Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, 2197 Mowry RD, Gainesville, FL 32611, United States. FAU - Lin, Zhoumeng AU - Lin Z AD - Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, 2197 Mowry RD, Gainesville, FL 32611, United States; Center for Environmental and Human Toxicology, University of Florida, 2187 Mowry RD, Gainesville, FL 32611, United States. Electronic address: linzhoumeng@ufl.edu. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - England TA - Food Chem Toxicol JT - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JID - 8207483 SB - IM COIS- Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. ☐ The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:32 PHST- 2026/02/05 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/10/27 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:32 [entrez] AID - S0278-6915(26)00546-6 [pii] AID - 10.1016/j.fct.2026.116471 [doi] PST - aheadofprint SO - Food Chem Toxicol. 2026 Oct 27:116471. doi: 10.1016/j.fct.2026.116471. PMID- 42946036 OWN - NLM STAT- Publisher LR - 20261027 IS - 1090-2430 (Electronic) IS - 0014-4886 (Linking) DP - 2026 Oct 27 TI - The deubiquitinase OTUD1 aggravated dopaminergic neurodegeneration in Parkinson's disease model by promoting the degradation of Parkin. PG - 116080 LID - S0014-4886(26)00448-6 [pii] LID - 10.1016/j.expneurol.2026.116080 [doi] AB - Parkin plays a pivotal role in the pathogenesis of Parkinson's disease (PD). Deubiquitinating enzymes (DUBs), which reverse Parkin ubiquitination, are critical for maintaining mitochondrial homeostasis. Although ovarian tumor protease deubiquitinase 1 (OTUD1) participates in various biological processes, its specific functions in PD and its regulation of Parkin remain unclear. In this study, we demonstrated that OTUD1 expression was significantly elevated in the nigrostriatal region of PD patients and MPTP-induced PD mice. Genetic ablation of OTUD1 markedly improved motor deficits and attenuated dopaminergic neuronal loss in PD models. Mechanistically, OTUD1 worsened mitochondrial dysfunction in PD models by negatively regulating Parkin protein levels. OTUD1 interacted with Parkin and selectively removed K27- and K33-linked polyubiquitin chains through its DUB activity, leading to Parkin degradation via the autophagic-lysosomal pathway. Our findings identify OTUD1 as a negative regulator of Parkin and reveal a mechanism linking deubiquitination to mitochondrial dysfunction in PD, which offers a novel potential therapeutic target for PD. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Wu, Mengting AU - Wu M AD - Shenzhen Research Institute of Shandong University, A301 Virtual University Park in South District of Shenzhen, China; Department of Pharmacology, School of Basic Medical Sciences and Qilu Hospital, Shandong University, Jinan 250012, China. FAU - Liu, Tengfei AU - Liu T AD - Shenzhen Research Institute of Shandong University, A301 Virtual University Park in South District of Shenzhen, China; Department of Pharmacology, School of Basic Medical Sciences and Qilu Hospital, Shandong University, Jinan 250012, China; Research Center for Basic Medical Sciences, Qilu Hospital, Shandong University, Jinan 250012, China. FAU - Zong, Runzhe AU - Zong R AD - Shenzhen Research Institute of Shandong University, A301 Virtual University Park in South District of Shenzhen, China; Department of Pharmacology, School of Basic Medical Sciences and Qilu Hospital, Shandong University, Jinan 250012, China. FAU - Yu, Lu AU - Yu L AD - Shenzhen Research Institute of Shandong University, A301 Virtual University Park in South District of Shenzhen, China; Department of Pharmacology, School of Basic Medical Sciences and Qilu Hospital, Shandong University, Jinan 250012, China. FAU - Ji, Qiuran AU - Ji Q AD - Shenzhen Research Institute of Shandong University, A301 Virtual University Park in South District of Shenzhen, China; Department of Pharmacology, School of Basic Medical Sciences and Qilu Hospital, Shandong University, Jinan 250012, China. FAU - Sun, Ru AU - Sun R AD - Department of Pharmacy, Shandong Provincial Hospital Affiliated to Shandong First Medical University, 324 Jingwu Road, Huaiyin District, Jinan 250021, China. Electronic address: sunny991123@163.com. FAU - Chen, Lin AU - Chen L AD - Shenzhen Research Institute of Shandong University, A301 Virtual University Park in South District of Shenzhen, China; Department of Pharmacology, School of Basic Medical Sciences and Qilu Hospital, Shandong University, Jinan 250012, China. Electronic address: linchen@sdu.edu.cn. FAU - Liu, Huiqing AU - Liu H AD - Shenzhen Research Institute of Shandong University, A301 Virtual University Park in South District of Shenzhen, China; Department of Pharmacology, School of Basic Medical Sciences and Qilu Hospital, Shandong University, Jinan 250012, China. Electronic address: liuhuiqing@sdu.edu.cn. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Exp Neurol JT - Experimental neurology JID - 0370712 SB - IM OTO - NOTNLM OT - Deubiquitination OT - Mitochondrial dysfunction OT - OTUD1 OT - Parkin OT - Parkinson's disease EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:32 PHST- 2026/07/24 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:32 [entrez] AID - S0014-4886(26)00448-6 [pii] AID - 10.1016/j.expneurol.2026.116080 [doi] PST - aheadofprint SO - Exp Neurol. 2026 Oct 27:116080. doi: 10.1016/j.expneurol.2026.116080. PMID- 42946035 OWN - NLM STAT- Publisher LR - 20261027 IS - 1090-2430 (Electronic) IS - 0014-4886 (Linking) DP - 2026 Oct 27 TI - Rodent models of post-stroke depression: A comparative analysis of photothrombosis and endothelin-1 models. PG - 116099 LID - S0014-4886(26)00467-X [pii] LID - 10.1016/j.expneurol.2026.116099 [doi] AB - Reliable, repeatable and clinically translatable animal models are required to uncover pathological mechanisms underlying post-stroke depression (PSD) and develop targeted therapeutics. Traditional ischemic stroke models are limited by high mortality and large, variable infarcts that confound affective assessment, while PSD clinically presents as secondary affective dysfunction occurring in stroke survivors after cerebral infarction. Therefore, ideal preclinical PSD models must achieve controllable focal brain lesions and recapitulate persistent depressive-like phenotypes. In recent years, photothrombosis (PT) and endothelin-1 (ET-1)-induced focal ischemia have become the most widely adopted rodent PSD paradigms, as they enable precise localized brain injury and recapitulate core behavioral and molecular characteristics of PSD. This review systematically compares PT and ET-1 models from multiple dimensions including surgical protocols, behavioral phenotypes, histopathological traits, and neurobiological mechanisms, and further summarizes standardized neurobehavioral and molecular detection assays. Direct comparative analysis reveals divergent strengths and drawbacks of the two models: the PT approach generates highly repeatable cortical infarcts and stable depressive-like behaviors but is limited to superficial brain tissues and lacks a well-defined ischemic penumbra; conversely, the ET-1 model allows stereotactic lesioning of subcortical nuclei, yet it yields inconsistent affective deficits and generally requires combined chronic stress to induce robust PSD-like phenotypes. Based on these findings, we propose two core principles for rational model selection: the primary determinant is whether the research focuses on cortical or subcortical neural circuits, and co-administration of chronic stress substantially improves the reliability and persistence of depressive phenotypes, although its necessity is contingent on species, strain, lesion site, and behavioral endpoint. Balancing lesion localization accuracy and behavioral stability during model choice will provide solid experimental support for subsequent PSD mechanistic research and preclinical drug screening. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Wang, Qingchen AU - Wang Q AD - Health Science Center, Ningbo University, 818 Fenghua Rd, Ningbo 315211, Zhejiang, China. FAU - Tang, Wenshi AU - Tang W AD - Health Science Center, Ningbo University, 818 Fenghua Rd, Ningbo 315211, Zhejiang, China. FAU - Xia, Yu AU - Xia Y AD - Health Science Center, Ningbo University, 818 Fenghua Rd, Ningbo 315211, Zhejiang, China. FAU - Teng, Jingning AU - Teng J AD - Health Science Center, Ningbo University, 818 Fenghua Rd, Ningbo 315211, Zhejiang, China. FAU - Tang, Lingjia AU - Tang L AD - Department of Geriatrics, Ningbo No.2 Hospital, Ningbo, Zhejiang 315000, China. Electronic address: tlj1114@126.com. FAU - Wang, Zhengchun AU - Wang Z AD - Health Science Center, Ningbo University, 818 Fenghua Rd, Ningbo 315211, Zhejiang, China. Electronic address: wangzhengchun@nbu.edu.cn. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - United States TA - Exp Neurol JT - Experimental neurology JID - 0370712 SB - IM OTO - NOTNLM OT - Behavioral phenotype OT - Endothelin-1 OT - Focal cerebral ischemia OT - Photothrombosis OT - Post-stroke depression OT - Rodents EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:32 PHST- 2026/07/14 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:32 [entrez] AID - S0014-4886(26)00467-X [pii] AID - 10.1016/j.expneurol.2026.116099 [doi] PST - aheadofprint SO - Exp Neurol. 2026 Oct 27:116099. doi: 10.1016/j.expneurol.2026.116099. PMID- 42946023 OWN - NLM STAT- Publisher LR - 20261027 IS - 1879-3150 (Electronic) IS - 0041-0101 (Linking) DP - 2026 Oct 27 TI - Response to "Fresh frozen plasma in snakebite-associated coagulopathy: study selection and the interpretation of a limited evidence base". PG - 109340 LID - S0041-0101(26)00358-2 [pii] LID - 10.1016/j.toxicon.2026.109340 [doi] FAU - Pati, Swagatika AU - Pati S AD - ICMR-Regional Medical Research Centre, Chandrashekharpur, Bhubaneswar, Odisha, India. Electronic address: swagatika.soma@gmail.com. FAU - Pattanayak, Priyanka Priyadarshini AU - Pattanayak PP AD - KIIT School of Public Health, KIIT Deemed to be University, Patia, Bhubaneswar, Odisha, India. Electronic address: priyankapattnayak26@gmail.com. FAU - Das, Debjani Priyadarsini AU - Das DP AD - KIIT School of Public Health, KIIT Deemed to be University, Patia, Bhubaneswar, Odisha, India. Electronic address: dasdebjanipriyadarsini@gmail.com. FAU - Banasmita, Banani AU - Banasmita B AD - KIIT School of Public Health, KIIT Deemed to be University, Patia, Bhubaneswar, Odisha, India. Electronic address: bananibanasmita419@gmail.com. FAU - Afeeq, K AU - Afeeq K AD - ICMR-Regional Medical Research Centre, Chandrashekharpur, Bhubaneswar, Odisha, India. Electronic address: afeeqk22@gmail.com. FAU - Sahu, Sameer AU - Sahu S AD - ICMR-Regional Medical Research Centre, Chandrashekharpur, Bhubaneswar, Odisha, India. Electronic address: sameersahu285.ss@gmail.com. FAU - Raj, Ranjan AU - Raj R AD - PGIMER & Capital Hospital, Bhubaneswar, Odisha, India. Electronic address: ranjanrazz66@gmail.com. FAU - Menon, Jaideep C AU - Menon JC AD - Amrita Institute of Medical Sciences, Amrita Vishwa Vidyapeetham, Kochi, Kerala, India. Electronic address: menon7jc@gmail.com. FAU - Palo, Subrata Kumar AU - Palo SK AD - ICMR-Regional Medical Research Centre, Chandrashekharpur, Bhubaneswar, Odisha, India. Electronic address: drpalsubrat@gmail.com. FAU - Rehman, Tanveer AU - Rehman T AD - ICMR-Regional Medical Research Centre, Chandrashekharpur, Bhubaneswar, Odisha, India. Electronic address: drtanveerrehman@gmail.com. FAU - Kanungo, Srikanta AU - Kanungo S AD - ICMR-Regional Medical Research Centre, Chandrashekharpur, Bhubaneswar, Odisha, India. Electronic address: srikantak109@gmail.com. FAU - Pati, Sanghamitra AU - Pati S AD - ICMR-Regional Medical Research Centre, Chandrashekharpur, Bhubaneswar, Odisha, India; Indian Council of Medical Research, New Delhi, India. Electronic address: drsanghamitra12@gmail.com. LA - eng PT - Letter DEP - 20261027 PL - England TA - Toxicon JT - Toxicon : official journal of the International Society on Toxinology JID - 1307333 SB - IM COIS- Declaration of Competing Interest ☒ The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:32 PHST- 2026/10/13 00:00 [received] PHST- 2026/10/23 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:32 [entrez] AID - S0041-0101(26)00358-2 [pii] AID - 10.1016/j.toxicon.2026.109340 [doi] PST - aheadofprint SO - Toxicon. 2026 Oct 27:109340. doi: 10.1016/j.toxicon.2026.109340. PMID- 42946020 OWN - NLM STAT- Publisher LR - 20261027 IS - 1097-6787 (Electronic) IS - 0190-9622 (Linking) DP - 2026 Oct 27 TI - Limited Familiarity, Limited Options: Ethical Challenges in Letters of Recommendation for Dermatology Residency. LID - S0190-9622(26)03828-4 [pii] LID - 10.1016/j.jaad.2026.10.066 [doi] FAU - Taghizadeh, Catherine S AU - Taghizadeh CS AD - Creighton University School of Medicine, Phoenix, AZ. FAU - Lipner, Shari R AU - Lipner SR AD - Department of Dermatology, Weill Cornell Medicine, New York, NY. Electronic address: shl9032@med.cornell.edu. LA - eng PT - Letter DEP - 20261027 PL - United States TA - J Am Acad Dermatol JT - Journal of the American Academy of Dermatology JID - 7907132 SB - IM OTO - NOTNLM OT - dermatology residency OT - educational equity OT - ethics OT - letter of recommendation OT - medical student mentorship OT - residency selection OT - standardized letter of evaluation EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:32 PHST- 2026/09/21 00:00 [received] PHST- 2026/10/20 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:32 [entrez] AID - S0190-9622(26)03828-4 [pii] AID - 10.1016/j.jaad.2026.10.066 [doi] PST - aheadofprint SO - J Am Acad Dermatol. 2026 Oct 27:S0190-9622(26)03828-4. doi: 10.1016/j.jaad.2026.10.066. PMID- 42946017 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1469-493X (Electronic) IS - 1361-6137 (Linking) VI - 10 IP - 10 DP - 2026 Oct 28 TI - Ergonomic interventions for treating work-related complaints of the arm, neck or shoulder in adults. PG - CD014644 LID - 10.1002/14651858.CD014644.pub2 [doi] LID - CD014644 AB - RATIONALE: Work-related complaints of the arm, neck or shoulder (CANS) are disorders that develop as a result of repetitive movements, awkward postures or external forces (e.g. vibrating tools), and significantly affect the working population globally. Ergonomic interventions (which aim to enhance efficiency and comfort in the working environment) can be used to manage CANS. This review is based on a new protocol but updates part of a 2013 Cochrane review. OBJECTIVES: To assess the effectiveness of ergonomic interventions for work-related complaints of the arm, neck or shoulder (CANS) in adults, in terms of their effects on pain, functional status and work-related outcomes. SEARCH METHODS: We updated the 2013 review searches, searching from 31 May 2013 to 1 June 2026 in eight bibliographic databases and one trials register. ELIGIBILITY CRITERIA: Randomised controlled trials (RCTs), cluster-RCTs and quasi-RCTs that evaluated single or combined ergonomic interventions (e.g. office furniture, biomechanical or ergonomic education) compared to no treatment/usual care/placebo or another active intervention (exercise/another ergonomic intervention/other treatment), or as an add-on treatment. OUTCOMES: Critical outcomes: pain, functional status (disability) and work-related outcomes (e.g. sickness absence, return to work, productivity loss at work, change of occupation). Important outcomes: global perceived effect (e.g. overall improvement), quality of life, healthcare consumption, and recurrence of injury, disorder or complaint. We prioritised outcomes in the short term (up to three months after the intervention) and were also interested in long-term results (over three months). RISK OF BIAS: We assessed the risk of bias in the studies using Cochrane's RoB 1 tool. SYNTHESIS METHODS: Two authors independently screened the search records, selected trials, extracted data and assessed risk of bias. When we judged studies to have sufficient clinical and statistical homogeneity, we performed meta-analysis. We assessed overall certainty of the evidence using GRADE for key outcomes at the short-term time point. INCLUDED STUDIES: We brought forward 13 studies from the 2013 review. We identified two new articles relating to one of those 13 studies, along with seven new studies. Therefore, in total, we included 20 studies involving 2478 participants. In 16 studies, the participants had non-specific CANS; in four studies, the participants had specific CANS (e.g. carpal tunnel syndrome). SYNTHESIS OF RESULTS: Results below are presented at the short-term time point. Ergonomic interventions versus no treatment, usual care or placebo in people with CANS Data were available only for trials that focused on non-specific CANS. Ergonomic interventions may result in little to no difference in pain compared to no treatment, usual care or placebo in people with CANS (SMD -0.23, 95% CI -0.54 to 0.08; 4 RCTs, 692 participants; low-certainty evidence). Ergonomic interventions probably result in little to no difference in functional status (disability) compared to being on a waiting list (RR 1.06, 95% CI 0.84 to 1.34; 1 RCT (2 comparisons), 383 participants; moderate-certainty evidence). Ergonomic interventions may result in little to no difference in sick leave compared to no treatment (MD for continuous data -0.32, 95% CI -0.73 to 0.09; 1 RCT, 98 participants; low-certainty evidence; RR for dichotomous data 0.87, 95% CI 0.63 to 1.21; 3 RCTs, 759 participants; moderate-certainty evidence). Ergonomic interventions probably result in little to no difference in recovery compared to no treatment (RR 1.32, 95% CI 0.89 to 1.97; 1 RCT (2 comparisons), 386 participants, moderate-certainty evidence). Ergonomic interventions may result in little to no difference in quality of life compared to no treatment (MD 0.10, 95% CI 0.00 to 0.20; 1 RCT, 68 participants; low-certainty evidence). Ergonomic interventions may result in little to no difference in healthcare consumption compared to no treatment when measured using continuous outcomes (MD -0.10. 95% CI -0.30 to 0.10; 1 RCT, 68 participants; low-certainty evidence), and may result in a reduction in healthcare consumption compared to usual care when measured using dichotomous outcomes (RR 0.49, 95% CI 0.31 to 0.78; 1 RCT, 210 participants; low-certainty evidence). No data were available for recurrence of injury, disorder or complaint. Ergonomic intervention versus active intervention (exercise or another type of ergonomic intervention or other treatment) in people with CANS Data were available from trials involving participants with specific CANS and with non-specific CANS. The evidence is very uncertain about the effects of ergonomic interventions on pain (SMD 0.41, 95% CI -0.02 to 0.84; 8 RCTs, 364 participants; very-low-certainty evidence) and on functional status (disability) (SMD -0.09, 95% CI -0.85 to 0.68; 3 RCTs, 180 participants; very-low-certainty evidence) compared to active intervention in people with specific or non-specific CANS. Intense ergonomic interventions may result in little to no difference in recovery (RR 1.29, 95% CI 0.85 to 1.94; 1 RCT, 257 participants; low-certainty evidence) or healthcare consumption (RR 0.86, 95% CI 0.50 to 1.47; 1 RCT, 217 participants; low-certainty evidence) compared to ergonomic education in people with non-specific CANS. Ergonomic interventions may improve quality of life compared to exercise in people with non-specific CANS (MD -10.10, 95% CI -19.87 to -0.33; 1 RCT, 61 participants; low-certainty evidence). No data were available for work-related outcomes or recurrence of injury, disorder or complaint. Ergonomic intervention plus another treatment versus the other treatment alone in people with CANS Data were available only from trials of participants with non-specific CANS. The evidence is very uncertain about the effects of ergonomic intervention as an add-on treatment to exercise on pain (SMD -0.50, 95% CI -1.64 to 0.63; 2 RCTs, 137 participants; very-low-certainty evidence). No data were available for our other outcomes of interest. AUTHORS' CONCLUSIONS: Overall, ergonomic interventions for work-related CANS may have no meaningful effects on pain, function, recovery, quality of life or work-related outcomes (sick leave) compared with no treatment or usual care. Findings are inconsistent for healthcare consumption. There may be a possible benefit for quality of life when ergonomic interventions are compared to exercise. The evidence for ergonomic interventions as an add-on treatment is very uncertain. High-quality, well-designed trials are needed to establish the role of ergonomic interventions in the management of CANS. FUNDING: The Danish Working Environment Research fund - Arbejdsmiljoforskningsfonden - project no 10-2022-03 REGISTRATION: Protocol (2022) DOI: 10.1002/14651858.CD014644. CI - Copyright (c) 2026 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd. FAU - Mehta, Poonam AU - Mehta P AUID- ORCID: 0000-0002-9307-9648 AD - Discipline of Physiotherapy, School of Human Performance, Rehabilitation and Population Health, Faculty of Health, University of Technology Sydney, Sydney, Australia. FAU - Stubbs, Peter W AU - Stubbs PW AD - Discipline of Physiotherapy, School of Human Performance, Rehabilitation and Population Health, Faculty of Health, University of Technology Sydney, Sydney, Australia. FAU - Tamminga, Sietske J AU - Tamminga SJ AD - Department of Public and Occupational Health, Amsterdam UMC, Location AMC, Amsterdam, Netherlands. FAU - Trostrup, Jeanette AU - Trostrup J AD - The Danish Healthcare Quality Institute, Aarhus, Denmark. FAU - Stynes, Siobhan M AU - Stynes SM AD - Arthritis Research UK Primary Care Centre, Keele University, Keele, UK. FAU - Koes, Bart W AU - Koes BW AD - Center for Muscle and Health, University of Southern Denmark, Odense, Denmark. FAU - Verhagen, Arianne P AU - Verhagen AP AD - Discipline of Physiotherapy, School of Human Performance, Rehabilitation and Population Health, Faculty of Health, University of Technology Sydney, Sydney, Australia. LA - eng PT - Journal Article PT - Meta-Analysis PT - Review PT - Systematic Review DEP - 20261028 PL - England TA - Cochrane Database Syst Rev JT - The Cochrane database of systematic reviews JID - 100909747 SB - IM UOF - doi: 10.1002/14651858.CD014644 MH - Humans MH - Randomized Controlled Trials as Topic MH - *Ergonomics/methods MH - Adult MH - *Shoulder Pain/therapy MH - *Occupational Diseases/therapy MH - *Neck Pain/therapy MH - *Cumulative Trauma Disorders/therapy PMC - PMC13713164 COIS- PM: none known. PS: none known. ST: none known. JT: none known. SBZ: none known. SS: none known. BK: none known. APV: none known. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 PMCR- 2027/10/28 CRDT- 2026/10/27 20:23 PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:23 [entrez] PHST- 2027/10/28 00:00 [pmc-release] AID - CD014644.pub2 [pii] AID - 10.1002/14651858.CD014644.pub2 [doi] PST - epublish SO - Cochrane Database Syst Rev. 2026 Oct 28;10(10):CD014644. doi: 10.1002/14651858.CD014644.pub2. PMID- 42946006 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1678-4227 (Electronic) IS - 0004-282X (Print) IS - 0004-282X (Linking) VI - 84 IP - 11 DP - 2026 Nov TI - Measuring the impact of migraine: a systematic review of the measurement properties of MIDAS, HIT-6, and MSQoL. PG - 1-11 LID - 10.1055/s-0046-1829149 [doi] LID - s00461829149 AB - BACKGROUND: Migraine is a leading cause of global disability. Valid, reliable instruments are essential for quantifying its impact. The Migraine Disability Assessment (MIDAS), Headache Impact Test (HIT-6), and Migraine-Specific Quality-of-Life questionnaire (MSQoL) are widely used, yet their comparative measurement properties across populations remain insufficiently characterized. OBJECTIVE: To systematically review and compare the measurement properties of MIDAS, HIT-6, and MSQoL following the Consensus-Based Standards for the Selection of Health Measurement Instruments (COSMIN) methodology. METHODS: This systematic review followed the COSMIN guidelines for systematic reviews of outcome measurement instruments and was reported per the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA)-COSMIN guideline. In total, seven databases were searched from study inception to January 31, 2025, restricted to English-language full-text studies. Methodological quality was appraised with the COSMIN Risk of Bias checklist; results were rated against criteria for good measurement properties, and evidence was graded using a modified Grading of Recommendations, Assessments, Development, and Evaluation (GRADE) approach. RESULTS: Of 2,674 records, 22 studies (20,455 participants) were included. All three instruments showed sufficient internal consistency and construct validity. The HIT-6 showed sufficient ratings across all properties (moderate-to-high quality evidence). Also, the MIDAS showed sufficient core properties but limited responsiveness and inconsistent reliability across cultural versions. Finally, MSQoL showed sufficient structural validity and internal consistency but insufficient test-retest reliability. CONCLUSION: Both HIT-6 and MIDAS can be recommended for measuring migraine impact (Category A), whereas MSQoL shows potential but requires further reliability and responsiveness research (Category B). A combined approach is recommended for comprehensive migraine burden evaluation. CI - The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution 4.0 International License, permitting copying and reproduction so long as the original work is given appropriate credit (https://creativecommons.org/licenses/by/4.0/). FAU - Prasad, Shilpa AU - Prasad S AUID- ORCID: 0009-0005-2387-6413 AD - Vinayaka Missions Research Foundation (VMRF-DU), Vinayaka Mission's Homoeopathic Medical College and Hospital (VMHMCH), Department of Case Taking and Repertory, India. FAU - Elaiyappan, Rathnasabapathi AU - Elaiyappan R AUID- ORCID: 0000-0002-3416-2644 AD - Vinayaka Missions Research Foundation (VMRF-DU), Vinayaka Mission's Homoeopathic Medical College and Hospital (VMHMCH), Department of Pediatrics, India. FAU - Hariram, Venkatesan AU - Hariram V AUID- ORCID: 0000-0003-2237-7071 AD - Vinayaka Missions Research Foundation (VMRF-DU), Vinayaka Mission's Homoeopathic Medical College and Hospital (VMHMCH), Department of Practice of Medicine, India. FAU - Ramalingam, Sitharthan AU - Ramalingam S AUID- ORCID: 0000-0003-4642-6042 AD - National Homoeopathy Research Institute in Mental Health, Department of Practice of Medicine, India. FAU - Arul, Vettrivel AU - Arul V AUID- ORCID: 0000-0002-2319-726X AD - Vinayaka Missions Research Foundation (VMRF-DU), Vinayaka Mission's Homoeopathic Medical College and Hospital (VMHMCH), Department of Community Medicine, India. LA - eng PT - Journal Article PT - Systematic Review DEP - 20261027 PL - Germany TA - Arq Neuropsiquiatr JT - Arquivos de neuro-psiquiatria JID - 0125444 SB - IM MH - *Migraine Disorders/psychology/physiopathology MH - Humans MH - *Quality of Life MH - *Disability Evaluation MH - Reproducibility of Results MH - Surveys and Questionnaires/standards PMC - PMC13713168 COIS- The authors have no conflict of interest to declare. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 PMCR- 2026/10/01 CRDT- 2026/10/27 20:13 PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:13 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - ANP-26-0246 [pii] AID - s00461829149 [pii] AID - 10.1055/s-0046-1829149 [doi] PST - ppublish SO - Arq Neuropsiquiatr. 2026 Nov;84(11):1-11. doi: 10.1055/s-0046-1829149. Epub 2026 Oct 27. PMID- 42946002 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1678-4227 (Electronic) IS - 0004-282X (Print) IS - 0004-282X (Linking) VI - 84 IP - 12 DP - 2026 Dec TI - The impact of rising global temperatures on neurological diseases. PG - 1-7 LID - 10.1055/s-0046-1829128 [doi] LID - s00461829128 AB - BACKGROUND: Climate change, driven predominantly by anthropogenic actions, represents a defining challenge of the 21st century. While its general health implications are well documented, the specific impact of global warming on neurological diseases has remained underemphasized for decades. OBJECTIVE: To evaluate the association between rising global temperatures and the exacerbation or incidence of various neurological conditions through a systematic review. METHODS: A systematic search was conducted in the PubMed/MEDLINE and SciELO databases for studies published between 2011 and 2026. Following strict eligibility criteria, 13 peer-reviewed articles were selected for qualitative synthesis. RESULTS: The evidence reveals that rising temperatures are increasingly linked to the worsening of conditions such as epilepsy, stroke, multiple sclerosis (MS), and neurodegenerative disorders like Alzheimer's and Parkinson's diseases. Specifically, heat exposure is associated with higher hospitalization rates and acute symptom exacerbation, such as Uhthoff's phenomenon in MS and autonomic thermoregulatory failure in Parkinson's. Although some geographic and methodological discrepancies exist, particularly regarding stroke and seizure frequency, the overall trend indicates increased neurological vulnerability to thermal stress. CONCLUSION: While research is expanding, neurological diseases remain an understudied frontier of the climate crisis. Targeted scientific investigation and robust public health strategies are imperative to mitigate the emerging neurological risks of a warming planet. CI - The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution 4.0 International License, permitting copying and reproduction so long as the original work is given appropriate credit (https://creativecommons.org/licenses/by/4.0/). FAU - Pereira, Gabriela Monteiro AU - Pereira GM AUID- ORCID: 0000-0003-4284-7691 AD - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas, Departamento de Neurologia, Brazil. FAU - Rocha, Gustavo Wagner Baratti AU - Rocha GWB AUID- ORCID: 0009-0001-2964-7380 AD - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas, Departamento de Neurologia, Brazil. FAU - Li, Li Min AU - Li LM AUID- ORCID: 0000-0001-6062-4649 AD - Universidade Estadual de Campinas, Faculdade de Ciencias Medicas, Departamento de Neurologia, Brazil. LA - eng PT - Journal Article PT - Systematic Review DEP - 20261027 PL - Germany TA - Arq Neuropsiquiatr JT - Arquivos de neuro-psiquiatria JID - 0125444 SB - IM MH - Humans MH - *Nervous System Diseases/etiology/epidemiology MH - *Global Warming MH - *Hot Temperature/adverse effects MH - Risk Factors MH - *Climate Change PMC - PMC13713171 COIS- The authors have no conflict of interest to declare. Li M. Li serves as President of the Green Party in Campinas, Brazil, which may be perceived as a non-financial interest related to environmental and climate-related topics discussed in this manuscript. This role did not influence the design, analysis, or conclusions of the study. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 PMCR- 2026/10/01 CRDT- 2026/10/27 20:12 PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:12 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - ANP-26-0133 [pii] AID - s00461829128 [pii] AID - 10.1055/s-0046-1829128 [doi] PST - ppublish SO - Arq Neuropsiquiatr. 2026 Dec;84(12):1-7. doi: 10.1055/s-0046-1829128. Epub 2026 Oct 27. PMID- 42945999 OWN - NLM STAT- Publisher LR - 20261027 IS - 1439-359X (Electronic) IS - 0939-7248 (Linking) DP - 2026 Oct 27 TI - Early Vaginal Reconstruction in Complex Anorectal Malformations: Rationale for an Individualized Approach. LID - 10.1055/a-2984-7286 [doi] AB - BACKGROUND: Current trends in reconstructive surgery increasingly advocate delaying vaginal reconstruction until patients can participate in decision-making. While this approach may be appropriate for conditions such as Mayer-Rokitansky-Kuster-Hauser syndrome and isolated vaginal agenesis, its application to patients with complex anorectal malformations (ARM), requires careful consideration. METHODS: This narrative review discusses the anatomical, surgical, and psychosocial factors influencing the timing of vaginal reconstruction in patients with complex anorectal malformations. Drawing on contemporary surgical principles and extensive clinical experience with reconstruction, differences between ARM and other causes of vaginal atresia are examined, together with reconstructive options and emerging strategies to preserve future reproductive potential. RESULTS: Patients with complex ARM differ substantially from patients with isolated vaginal agenesis. They frequently present with associated urinary tract anomalies, neurogenic bladder, fecal incontinence as well as previous abdominal and perineal operations. In selected patients, vaginal reconstruction performed during the initial repair offers important technical advantages, including optimal pelvic exposure before fibrosis develops, preservation of surgical options, and avoidance of later operations in a hostile pelvis. The choice of reconstructive tissue should be individualized according to anatomy and anticipated bowel function. Native vaginal tissue remains the preferred option whenever available; however, when replacement is necessary, the rectum provides the most favorable intestinal substitute in appropriately selected patients, followed by colon, whereas small bowel should generally be reserved for situations in which other options are unavailable. CONCLUSIONS: Delayed vaginal reconstruction should not be considered the universal standard for patients with anorectal malformations. Surgical timing should be individualized according to anatomical complexity, predicted bowel function, future urological procedures, patient circumstances, and long-term quality-of-life considerations. Management within specialized multidisciplinary centers remains essential for optimizing both functional and reproductive outcomes. CI - Thieme. All rights reserved. FAU - Bischoff, Andrea AU - Bischoff A AUID- ORCID: 0009-0007-6437-4967 AD - University of Colorado Anschutz Medical Campus, Pediatric Surgery, Colorado, United States, Aurora. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Eur J Pediatr Surg JT - European journal of pediatric surgery : official journal of Austrian Association of Pediatric Surgery ... [et al] = Zeitschrift fur Kinderchirurgie JID - 9105263 SB - IM OTO - NOTNLM OT - anorectal malformation; cloaca; vaginal reconstruction; neovagina; colorectal surgery; pediatric surgery. COIS- The authors declare that they have no conflict of interest. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 20:03 PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 20:03 [entrez] AID - 10.1055/a-2984-7286 [doi] PST - aheadofprint SO - Eur J Pediatr Surg. 2026 Oct 27. doi: 10.1055/a-2984-7286. PMID- 42945982 OWN - NLM STAT- Publisher LR - 20261027 IS - 2468-2667 (Electronic) DP - 2026 Oct 27 TI - Global, regional, and national burden of unintentional poisoning, 1990-2023: results from the Global Burden of Disease Study 2023. LID - S2468-2667(26)00173-8 [pii] LID - 10.1016/S2468-2667(26)00173-8 [doi] AB - BACKGROUND: Unintentional poisoning is a preventable cause of injury-related morbidity and mortality, yet global patterns, trends, and inequalities remain incompletely characterised. Within the Global Burden of Disease (GBD) framework, unintentional poisoning includes poisoning by carbon monoxide and poisoning by other means, a category largely informed by pesticide poisoning codes plus some indirect capture of other poisoning as part of the GBD re-distribution process. Poisonings by pharmaceuticals and illicit substances are not captured in the GBD definition of poisoning. We aimed to quantify this GBD-defined burden and assess regional variation, sociodemographic gradients, and case fatality between 1990 and 2023. METHODS: We analysed GBD 2023 estimates for 204 countries and territories, modelling deaths, incidence, and disability-adjusted life-years (DALYs) using standard GBD cause-of-death and non-fatal estimation methods with 95% uncertainty intervals. We stratified results by age, sex, region, and Socio-demographic Index (SDI), and derived case fatality from modelled deaths and incidence. FINDINGS: In 2023, unintentional poisoning from carbon monoxide and other means caused 2.54 million incident poisonings, 69 805 deaths, and 3.68 million DALYs globally, with age-standardised DALY rates declining by 47.5% since 1990. Carbon monoxide accounted for 641 932 poisonings, 30 890 deaths and 1.34 million DALYs, with the highest burdens in central and eastern Europe and central Asia. Most regions showed substantial long-term declines, and the global age-standardised DALY rate for carbon monoxide poisoning declined by 58.2% since 1990. Poisoning by other means caused 1.90 million incident cases, 38 914 deaths, and 2.34 million DALYs, with the greatest burden in sub-Saharan Africa. Most regions showed declining age-standardised DALY rates for poisoning by other means; however, there was no clear change (with the 95% uncertainty interval including zero) in the global estimate, driven by wide uncertainty intervals in several regions (including sub-Saharan Africa). Mortality and DALYs were inversely associated with SDI, and derived case fatality was highest in low-SDI regions. INTERPRETATION: Global reductions in GBD-defined unintentional poisoning by carbon monoxide and other means are consistent with decades of regulatory and environmental interventions. These include bans of highly toxic pesticides, improved heating and ventilation systems, emissions controls, and adoption of carbon monoxide alarms. Stark inequalities persist in some regions and in low-SDI settings. Compared with previous GBD publications, this study provides a detailed global assessment of unintentional poisoning by carbon monoxide and other means up to 2023, integrating incidence, DALYs, and comparative case fatality across regions. FUNDING: Gates Foundation and the Australian National Health and Medical Research Council. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY 4.0 license. Published by Elsevier Ltd.. All rights reserved. CN - GBD 2023 Unintentional Poisoning Collaborators LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Lancet Public Health JT - The Lancet. Public health JID - 101699003 SB - IM COIS- Declaration of interests R Cairns reports a grant from Reckitt, outside the submitted work; and payment or honoraria for lectures from Reckitt and the Pharmacy Guild of Australia, outside the submitted work. S Afzal reports support for the present manuscript-the study material, manuscripts, medical writings, and library resources are provided by Institute of Public Health Lahore for this manuscript; intellectual contributions and time protection was given by Dean office Institute of Public Health Lahore; honoraria for experts, lectures, visiting speakers and educational seminars were provided by the Dean Institute of Public Health Lahore; support for attending meetings and travel was provided by the Dean Institute of Public Health, Lahore Pakistan; participation on a data safety monitoring board or advisory board as Member Prime Minister Taskforce for Elimination of Infectious Diseases, Member Prime Minister Technical Experts Group for Health Registeries and Analytics, Member and co-chair, Pakistan National Bioethics Committee, Member Institutional Review Board of King Edward Medical University, Member Fatima Jinnah Medical University, Member Ethical Review Board and Data Monitoring Board Institute of Public Health Lahore Pakistan, In charge Clinical Research Organization King Edward Medical University, Member Annals of King Edward Medical University Advisory Board; leadership or fiduciary role in other board, society, committee or advocacy group, paid or unpaid as Member Pakistan Higher Education Commission Research Committee, Member Pakistan Medical and Dental Commission Research and Journals Committee, Member Pakistan National Bioethics Committee, Member Pakistan Society of Internal Medicine, Member Pakistan Association of Medical Editors, Member Medical Microbiology and Infectious Diseases Society, Fellow of Leads International, Fellow of Faculty of Public Health UK, Fellow of College of Physicians and Surgeons Pakistan; received computer software and equipment from Bergen University Norway for research writing; and other financial or non-financial interests as Dean Public Health Institute of Public Health Birdwood Lahore, Member National Bioethics Committee Pakistan, and Member Prime Minister Taskforce on HCV, HIV and Infectious Diseases, outside the submitted work. R Ancuceanu reports consulting fees from Merck Romania; payment or honoraria for lectures, presentations, speakers bureaus, manuscript writing or educational events from Laropharm, Reckitt, Merck Romania, MagnaPharm, Biessen Pharma, and ALK Slovakia; and support for attending meetings or travel from Merck Romania and Reckitt (all no relationship with the current topic), outside the submitted work. M Aslam reports grants or contracts from Xiamen University Malaysia Research Fund (XMUMRF), grant number: XMUMRF/2025-C15/ITCM/0006 (Therapeutic and Toxicity Evaluation of Selected Medicinal Herbs for NAFLD: Exploring the Inter-Organelle Contact Sites Modulation Theory; co-investigator; January, 2025, to December, 2027, ongoing), grant number: XMUMRF/2023-C11/ISEM/0041 (Children's Rights Education in the Early Years of Divorce: An Exploration of Adolescents' Perspectives; co-investigator; January, 2023, to December, 2025, ongoing), and grant number: XMUMRF/2026-C17/ITCM/0007 (Olfactory Stimulation and Autonomic Regulation in Menstrual Disorders; co-investigator; January, 2026, to December, 2028, ongoing; all internal XMUMRF research grants administered by Xiamen University Malaysia; funds disbursed to institutional research account only; no salary, honoraria, or personal payments to author), outside the submitted work. M Carvalho reports other financial or non-financial interests with LAQV/REQUIMTE, University of Porto, Porto, Portugal (support from FCT/MCTES under the scope of the project UID/50006/2025, DOI 10.54499/UID/50006/2025), outside the submitted work. P Dhanaraj reports support for the present manuscript-manuscript feedback, rewriting, and review. D Dias da Silva reports grants or contracts from FCT - Fundacao para a Ciencia e a Tecnologia (Project 2024.06933.RESTART, payment made to DDdS's institution to support DDdS's research) and from E2S|P.Porto - Escola Superior de Saude do Politecnico do Porto (tenure position as an Adjunct Professor); payment or honoraria for lectures, presentations, speakers bureaus, manuscript writing or educational events from FFUP - Faculdade de Farmacia da Universidade do Porto (Portugal) and from FMUP - Faculdade de Medicina da Universidade do Porto (Portugal), both payments made to DDdS's institution for DDdS's lectures; and support for attending meetings or travel from E2S|P.Porto - Escola Superior de Saude do Politecnico do Porto, Portugal and from Research Unit LAQV-REQUIMTE, Portugal (both travel and meetings expenses), outside the submitted work. I El-Bayoumy reports leadership or fiduciary role in other board, society, committee or advocacy group, paid or unpaid as Editor-in-Chief for recent trends in infectious diseases-STM Journals and as Editorial member of Texila American university public health Journal South America, outside the submitted work. A Faro reports support for the present manuscript from National Council for Scientific and Technological Development (CNPq; CNPq Research Productivity Fellow). R Franklin reports support for attending meetings or travel from ACTM Annual Conference 2024-26; and leadership or fiduciary role in other board, society, committee or advocacy group, paid or unpaid as President of Australasian College of Tropical Medicine, President of Kidsafe Australia, Board Member Royal Life Saving Society Australia, Board Member Auschem Training, and Trustee at Royal Life Saving Commonwealth, outside the submitted work. I Ilic reports support for the present manuscript from Ministry of Science, Technological Development and Innovation of the Republic of Serbia (number 451-03-137/2025-03/200110). K Krishan reports other financial or non-financial interests; and acknowledges non-financial support from RUSA 2.0 grant awarded by Ministry of Education to Panjab University, Chandigarh, India, outside the submitted work. M Li reports grants or contracts from the National Science and Technology Council, Taiwan (NSTC 115-2410-H-003-022; 115-2621-M-003-002); and leadership or fiduciary role in other board, society, committee or advocacy group, paid or unpaid as Technical Editor, Journal of the American Heart Association, outside the submitted work. S Panda reports support for the present manuscript from Siksha 'O' Anusandhan (deemed to be University; payment of salary); and grants or contracts file number 17-59/2023-24/CCRH/Tech./Coll./ICMR-Diabetes/960 (as co-investigator) and File number HS/RC/393 (as principal investigator), outside the submitted work. A Peden reports support for the present manuscript from [Australian] National Health and Medical Research Council (grant number: APP2009306). E Pirera reports support for attending meetings and/or travel from Chiesi Farmaceutici, Menarini, Sanofi Regeneron; and other financial or non-financial interests as external expert for GlaxoSmithKline, outside the submitted work. Y Samodra reports grants or contracts from NTU EPM-NSTC, Taiwan and from RCEC, Academia Sinica, Taiwan (both post-doctoral fellow contract); consulting fees from Jago Beasiswa (https://idebeasiswa.com; international scholarships consultancy); and leadership or fiduciary role in other board, society, committee or advocacy group, paid or unpaid with Benang Merah Research Center (https://benangmerah.net; as a co-founder), outside the submitted work. V Sharma reports other financial or non-financial interests; acknowledges support from DFSS (MHA)'s research project (DFSS28(1)2019/EMR/6) at Department of Forensic Science (Formerly Institute of Forensic Science & Criminology), Panjab University, Chandigarh, India, RUSA 2.0 grant from Ministry of Education to Panjab University and PAIR grant (ANRF/PAIR/2025/000006/PAIR) to Panjab University from ANRF, outside the submitted work. V Shivarov reports payment or honoraria for lectures, presentations, speakers bureaus, manuscript writing or educational events from MSD, Novartis, Johnson and Johnson (lecturing honoraria; unrelated to this work); support for attending meetings or travel from AstraZeneca (travel grant; unrelated to this work); patents planned, issued or pending with Bulgarian Patent Office (one patent; unrelated to this work); and other financial or non-financial interests from ICON plc (salary; unrelated to this work), outside the submitted work. S Straube reports payment or honoraria for lectures, presentations, speakers bureaus, manuscript writing or educational events from Occupational Medicine Specialists of Canada (honoraria, payment to SS); and leadership or fiduciary role in other board, society, committee or advocacy group, paid or unpaid with SN Comprehensive Clinical Medicine (editorial board member) and with M.S.I. Foundation (board member, payments to SS), outside the submitted work. FIR - Cairns, Rose IR - Cairns R IRAD- Faculty of Medicine and Health, University of Sydney, Sydney, NSW, Australia; New South Wales Poisons Information Centre, The Children's Hospital at Westmead, Sydney, NSW, Australia. Electronic address: rose.cairns@sydney.edu.au. FIR - Buckley, Nicholas Allan IR - Buckley NA IRAD- Faculty of Medicine and Health, University of Sydney, Sydney, NSW, Australia. FIR - Abdalla, Ashraf Nabiel IR - Abdalla AN IRAD- College of Pharmacy, Umm Al-qura University, Makka, Saudi Arabia. FIR - Abdelkader, Atef IR - Abdelkader A IRAD- Department of Mathematics and Sciences, Ajman University, Ajman, United Arab Emirates. FIR - Abdelwahab, Siddig Ibrahim IR - Abdelwahab SI IRAD- Health Research Centre, Jazan University, Jazan, Saudi Arabia. FIR - Abdoun, Meriem IR - Abdoun M IRAD- Department of Medicine, University of Setif Algeria, Setif, Algeria; Department of Health, University of Setif Algeria, Setif, Algeria. FIR - Abdrakhmanova, Zinat IR - Abdrakhmanova Z IRAD- Health Research Institute, Al Farabi Kazakh National University, Almaty, Kazakhstan. FIR - Abu Lila, Amr Selim IR - Abu Lila AS IRAD- Department of Pharmaceutics, University of Hail, Hail, Saudi Arabia. FIR - Abuhelwa, Ahmad Y IR - Abuhelwa AY IRAD- Department Pharmacy Practice and Pharmacotherapeutics, University of Sharjah, Sharjah, United Arab Emirates. FIR - Abu-Zaid, Ahmed IR - Abu-Zaid A IRAD- Department of Biochemistry and Molecular Medicine, Alfaisal University, Riyadh, Saudi Arabia; College of Graduate Health Sciences, University of Tennessee, Memphis, TN, USA. FIR - Acharya, Apurba IR - Acharya A IRAD- Institute of International Health, Charite Medical University Berlin, Berlin, Germany. FIR - Adamu, Lawan Hassan IR - Adamu LH IRAD- Department of Human Anatomy, Federal University Dutse, Dutse, Nigeria; Department of Anatomy, Bayero University Kano, Kano, Nigeria. FIR - Adamu, Yau IR - Adamu Y IRAD- Kate Marimon School of Public Health, The University of Texas at San Antonio, San Antonio, TX, USA. FIR - Addo, Isaac Yeboah IR - Addo IY IRAD- School of Medicine, University of Sydney, Sydney, NSW, Australia; Centre for Social Research in Health, University of New South Wales, Sydney, NSW, Australia. FIR - Adebiyi, Babatope Oluwadamilare IR - Adebiyi BO IRAD- Department of Pediatrics, University of Calgary, Calgary, AB, Canada; School of Public Health, University of the Western Cape, Bellville, South Africa. FIR - Adedokun, Kamoru Ademola IR - Adedokun KA IRAD- Department of Immunology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA. FIR - Adekoya, Oluwatodimu Adewole IR - Adekoya OA IRAD- One-Health Science Department, Texas Tech University, Amarillo, TX, USA. FIR - Adeleke, Olumide Thomas IR - Adeleke OT IRAD- Department of Family Medicine, Bowen University, Iwo, Nigeria; Department of Family Medicine, Bowen University Teaching Hospital, Ogbomoso, Nigeria. FIR - Adepoju, Daniel Adeyemi IR - Adepoju DA IRAD- Department of Health Informatics, Indiana University Indianapolis, Indianapolis, IN, USA. FIR - Adesina, Miracle Ayomikun IR - Adesina MA IRAD- Slum and Rural Health Initiative Research Academy, Slum and Rural Health Initiative, Ibadan, Nigeria; Department of Physiotherapy, University of Ibadan, Ibadan, Nigeria. FIR - Adetunji, Charles Oluwaseun IR - Adetunji CO IRAD- Department of Pediatrics, University of Calgary, Calgary, AB, Canada. FIR - Adetunji, Juliana Bunmi IR - Adetunji JB IRAD- Department of Biochemistry, Osun State University, Osogbo, Nigeria. FIR - Adisu, Molalign Aligaz IR - Adisu MA IRAD- College of Health Science, Woldia University, Woldia, Ethiopia. FIR - Adjei, Franklin Akwasi IR - Adjei FA IRAD- College of Health Sciences, University of Wyoming, Laramie, WY, USA. FIR - Admassie, Belete Muluadam IR - Admassie BM IRAD- Department of Anesthesia, Bahir Dar University, Bahir Dar, Ethiopia. FIR - Adoma, Prince Owusu IR - Adoma PO IRAD- Department of Health Administration and Education, University of Education Winneba, Winneba, Ghana. FIR - Adonteng-Kissi, Obed IR - Adonteng-Kissi O IRAD- School of Arts and Social Sciences, Edith Cowan University, Bunbury, WA, Australia. FIR - Adotei, Naomi IR - Adotei N IRAD- St Luke Hospital, Kasei, Ministry of Health, Kumasi, Ghana. FIR - Afjal, Mohd Amir IR - Afjal MA IRAD- Department of Transplant Surgery, University of Tennessee, Memphis, TN, USA. FIR - Afolabi, Aanuoluwapo Adeyimika IR - Afolabi AA IRAD- Technical Services Directorate, MSI Nigeria Reproductive Choices, Abuja, Nigeria. FIR - Afzal, Saira IR - Afzal S IRAD- Department of Community Medicine, King Edward Memorial Hospital, Lahore, Pakistan; Department of Public Health, Public Health Institute, Lahore, Pakistan. FIR - Aggarwal, Navidha IR - Aggarwal N IRAD- MM College of Pharmacy, Maharishi Markandeshwar (Deemed to Be University), Ambala, India. FIR - Agide, Feleke Doyore IR - Agide FD IRAD- Department of Public Health, Kampala International University, Kampala, Uganda; School of Public Health, Wachemo University, Hossana, Ethiopia. FIR - Agoi, Mary Dada IR - Agoi MD IRAD- Department of Biosciences and Biotechnology, University of Medical Sciences, Ondo, Ondo City, Nigeria. FIR - Agordoh, Percival Delali IR - Agordoh PD IRAD- Department of Nutrition and Dietetics, University of Health and Allied Sciences, Ho, Ghana. FIR - Ahmad, Aqeel IR - Ahmad A IRAD- College of Medicine, Shaqra University, Shaqra, Saudi Arabia. FIR - Ahmad, Muayyad M IR - Ahmad MM IRAD- School of Nursing, The University of Jordan, Amman, Jordan. FIR - Ahmad, Rabbiya IR - Ahmad R IRAD- Department of Clinical Pharmacy, Universiti Sains Malaysia, Penang, Malaysia; Department of Pharmacy, The University of Lahore, Lahore, Pakistan. FIR - Ahmad, Sajjad IR - Ahmad S IRAD- Department of Biotechnology, Institute of Space Technology (IST), Islamabad, Pakistan. FIR - Ahmed, Ayman IR - Ahmed A IRAD- Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan; Pan-African One Health Institute (PAOHI), Kigali, Rwanda. FIR - Ahmed, Mushood IR - Ahmed M IRAD- Department of Medicine, Rawalpindi Medical University, Rawalpindi, Pakistan. FIR - Ahmed, Nesredin IR - Ahmed N IRAD- School of Nursing, Haramaya University, Harar, Ethiopia. FIR - Ahmed, Syed Anees IR - Ahmed SA IRAD- Brody School of Medicine, East Carolina University, Greenville, NC, USA. FIR - Akanni, Olukunmi Olatunji IR - Akanni OO IRAD- Department of Statistics, University of Ibadan, Ibadan, Nigeria. FIR - Akhigbe, Roland Eghoghosoa IR - Akhigbe RE IRAD- Department of Physiology, Ladoke Akintola University, Ogbomoso, Nigeria. FIR - Akintunde, Damilare Michael IR - Akintunde DM IRAD- Medecins Sans Frontieres - Holland, Doctors Without Borders, Omdurman, Sudan; Department of Community Medicine, Ahmadu Bello University, Zaria, Nigeria. FIR - Akintunde, Omotolani Olawunmi IR - Akintunde OO IRAD- Institute of Child Health, Usmanu Danfodiyo University, Sokoto, Sokoto, Nigeria. FIR - Al Hasan, Syed Mahfuz IR - Al Hasan SM IRAD- Division of Public Health Sciences, Washington University in St. Louis, St. Louis, MO, USA. FIR - Al Zoubi, Mohammad Ahmmad Mahmoud IR - Al Zoubi MAM IRAD- School of Public Health, University of Texas, Houston, TX, USA. FIR - Al-Ahmad, Mohammad M IR - Al-Ahmad MM IRAD- Clinical Pharmacy Department, Al Ain University, Al Ain, United Arab Emirates. FIR - Al-Ajlouni, Yazan IR - Al-Ajlouni Y IRAD- Department of Rehabilitation, Montefiore Medical Center, Bronx, NY, USA; Department of Epidemiology, Columbia University, New York, NY, USA. FIR - Alammar, Muath A IR - Alammar MA IRAD- Department of Family Medicine, Shaqra University, Shaqra, Saudi Arabia. FIR - Alarifi, Abdullah M IR - Alarifi AM IRAD- Deputyship of Population Health, Ministry of Health, Riyadh, Saudi Arabia; College of Medicine, Alfaisal University, Riyadh, Saudi Arabia. FIR - Al-Ashwal, Fahmi Y IR - Al-Ashwal FY IRAD- College of Pharmacy, Al-Ayen Iraqi University, Thi-Qar, Iraq; Department of Clinical Pharmacy and Pharmacy Practice, University of Science and Technology, Sana'a, Yemen. FIR - Albashtawy, Mohammed IR - Albashtawy M IRAD- Department of Community and Mental Health, Al al-Bayt University, Mafraq, Jordan. FIR - Albikawi, Zainab Fatehi IR - Albikawi ZF IRAD- Faculty of Nursing, Yarmouk University, Irbid, Jordan. FIR - Aleidi, Shereen M IR - Aleidi SM IRAD- College of Pharmacy, University of Sharjah, Sharjah, United Arab Emirates; School of Pharmacy, The University of Jordan, Amman, Jordan. FIR - Al-Eyadhy, Ayman IR - Al-Eyadhy A IRAD- Pediatric Intensive Care Unit, King Saud University, Riyadh, Saudi Arabia. FIR - Alfalki, Ali M IR - Alfalki AM IRAD- Department of Epidemiology and Biostatistics, University of South Carolina, Columbia, SC, USA. FIR - Alhasan, Khalid A IR - Alhasan KA IRAD- Pediatrics Department, King Saud University, Riyadh, Saudi Arabia; Kidney and Pancreas Health Center, King Faisal Specialist Hospital & Research Center, Riyadh, Saudi Arabia. FIR - Alhawajreh, Mohammad Jamil IR - Alhawajreh MJ IRAD- School of Health Sciences, Hamdan Bin Mohammed Smart University, Dubai, United Arab Emirates. FIR - Ali, Maratab IR - Ali M IRAD- School of Food and Agricultural Sciences, University of Management and Technology, Lahore, Pakistan. FIR - Ali, Mohammad Daud IR - Ali MD IRAD- Department of Pharmacy, Mohammed Al-Mana College for Medical Sciences, Dammam, Saudi Arabia. FIR - Ali, Syed Shujait IR - Ali SS IRAD- Molecular Microbiology and Infectious Disease Unit, University of Tabuk, Tabuk, Saudi Arabia; University of Swat, Swat, Pakistan. FIR - Al-Iede, Montaha IR - Al-Iede M IRAD- The School of Medicine, The University of Jordan, Amman, Jordan. FIR - Al-Jabi, Samah W IR - Al-Jabi SW IRAD- Department of Clinical and Community Pharmacy, An-Najah National University, Nablus, Palestine. FIR - Allouh, Mohammed Z IR - Allouh MZ IRAD- Department of Anatomy, United Arab Emirates University, Al Ain, United Arab Emirates; Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan. FIR - Almarshadi, Fahad Awwadh IR - Almarshadi FA IRAD- Department of Public Health, University of Hail, Hail, Saudi Arabia. FIR - Al-Marwani, Sabah IR - Al-Marwani S IRAD- Independent Consultant, Amman, Jordan. FIR - Alnaeem, Mohmmad Minwer IR - Alnaeem MM IRAD- Department of Clinical Nursing, Al-Zaytoonah University of Jordan, Amman, Jordan. FIR - Alnawafleh, Khaldoon Aied IR - Alnawafleh KA IRAD- Nursing Faculty, Fahad Bin Sultan University, Tabuk, Saudi Arabia. FIR - Alniss, Hasan Yaser IR - Alniss HY IRAD- College of Pharmacy, University of Sharjah, Sharjah, United Arab Emirates. FIR - Alrimawi, Intima IR - Alrimawi I IRAD- Department of Nursing, Georgetown University, Washington, DC, USA. FIR - Alrousan, Sahel Majed IR - Alrousan SM IRAD- Macro-Fiscal Policy Department, Ministry of Finance, Dubai, United Arab Emirates. FIR - Alsaif, Bandar IR - Alsaif B IRAD- Public Health Department, University of Hail, Hail, Saudi Arabia. FIR - Alshahrani, Najim Z IR - Alshahrani NZ IRAD- Department of Family and Community Medicine, University of Jeddah, Jeddah, Saudi Arabia. FIR - Alsharari, Abdalkarem Fedgash IR - Alsharari AF IRAD- College of Nursing, Jouf University, Sakaka, Saudi Arabia. FIR - Alsukaibi, Abdulmohsen Kd IR - Alsukaibi AK IRAD- Department of Chemistry, University of Hail, Hail, Saudi Arabia. FIR - Alwafi, Hassan IR - Alwafi H IRAD- Department of Clinical Pharmacology and Toxicology, Umm Al-Qura University, Makkah, Saudi Arabia. FIR - Al-Worafi, Yaser Mohammed IR - Al-Worafi YM IRAD- Fakeeh College for Medical Sciences, Jeddah, Saudi Arabia; College of Medical Sciences, Azal University for Human Development, Sana'a, Yemen. FIR - Alzoubi, Abdallah IR - Alzoubi A IRAD- Faculty of Medicine, Jordan University of Science and Technology, Irbid, Jordan. FIR - Al-Zubairi, Adel S IR - Al-Zubairi AS IRAD- Department of Biochemistry and Molecular Biology, Sana'a University, Sana'a, Yemen; Laboratory Medicine Department, Al-Baha University, Al-Aqiq, Saudi Arabia. FIR - Aman Mohammadi, Masoud IR - Aman Mohammadi M IRAD- Food and Beverages Safety Research Center, Urmia University of Medical Sciences, Urmia, Iran. FIR - Amin, Amr IR - Amin A IRAD- College of Medicine, University of Sharjah, Sharjah, United Arab Emirates; Biological Sciences Collegiate Division, Cairo University, Cairo, Egypt. FIR - Ancuceanu, Robert IR - Ancuceanu R IRAD- Faculty of Pharmacy, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania. FIR - Angaw, Yonas IR - Angaw Y IRAD- Department of Public Health, Adigrat University, Adigrat, Ethiopia. FIR - Anuoluwa, Boluwatife Stephen IR - Anuoluwa BS IRAD- Department of Nursing, Georgetown University, Washington, DC, USA. FIR - Anvari, Saeid IR - Anvari S IRAD- Regenerative Medicine, Organ Procurement and Transplantation Multi-disciplinary Center, Guilan University of Medical Sciences, Rasht, Iran. FIR - Areda, Demelash IR - Areda D IRAD- College of Art and Science, Ottawa University, Surprise, AZ, USA. FIR - Aremu, Abdulfatai IR - Aremu A IRAD- Department of Veterinary Pharmacology and Toxicology, University of Ilorin, Ilorin, Nigeria. FIR - Arthur-Holmes, Francis IR - Arthur-Holmes F IRAD- Faculty of Business and Law, University of Portsmouth, Portsmouth, UK. FIR - Asdaq, Syed Mohammed Basheeruddin IR - Asdaq SMB IRAD- Department of Pharmacy Practice, AlMaarefa University, Riyadh, Saudi Arabia. FIR - Aslam, Muhammad Shahzad IR - Aslam MS IRAD- School of Traditional Chinese Medicine, Xiamen University Malaysia, Sepang, Malaysia. FIR - Asmar, Imad IR - Asmar I IRAD- Department of Nursing, Birzeit University, Birzeit, Palestine. FIR - Astuti, Ratna Dwi Puji IR - Astuti RDP IRAD- Department of Environmental Health, Universitas Airlangga, Surabaya, Indonesia. FIR - Athari, Seyyed Shamsadin IR - Athari SS IRAD- Department of Immunology, Zanjan University of Medical Sciences, Zanjan, Iran. FIR - Atreya, Alok IR - Atreya A IRAD- Department of Forensic Medicine, Lumbini Medical College, Palpa, Nepal. FIR - Atsbaha, Abadi Hailay IR - Atsbaha AH IRAD- College of Medicine and Health Sciences, Adigrat University, Adigart, Ethiopia. FIR - Atta, Julie Alaere IR - Atta JA IRAD- Management Policy and Community Health, University of Texas, Houston, TX, USA. FIR - Awol, Kemal Lemnuro IR - Awol KL IRAD- School of Medicine, Werabe University, Werabe, Ethiopia; School of Medicine, Johns Hopkins University, Baltimore, MD, USA. FIR - Ayed, Ahmad IR - Ayed A IRAD- Faculty of Nursing, Arab American University Palestine, Jenin, Palestine. FIR - Aziz, Mohd Yusmaidie IR - Aziz MY IRAD- Pusat Kanser Tun Abdullah Ahmad Badawi, Universiti Sains Malaysia, Penang, Malaysia. FIR - Baczek, Tomasz IR - Baczek T IRAD- Department of Pharmaceutical Chemistry, Medical University of Gdansk, Gdansk, Poland; Institute of Health Sciences, Pomeranian University in Slupsk, Slupsk, Poland. FIR - Badar, Muhammad IR - Badar M IRAD- Gomal Center of Biochemistry and Biotechnology, Gomal University, Dera Ismail Khan, Pakistan. FIR - Badiye, Ashish D IR - Badiye AD IRAD- Department of Forensic Science, Government Institute of Forensic Science Nagpur, Nagpur, India; Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, India. FIR - Badraoui, Riadh IR - Badraoui R IRAD- Faculty of Medicine of Tunis, University Tunis El Manar, Tunis, Tunisia; Department of Biology, University of Hail, Hail, Saudi Arabia. FIR - Bagheri, Nasser IR - Bagheri N IRAD- Health Research Institute, University of Canberra, Canberra, NSW, Australia. FIR - Baghlaf, Khlood K IR - Baghlaf KK IRAD- Pediatric Dentistry Department, King Abdulaziz University, Jeddah, Saudi Arabia. FIR - Bahar, Abdul Rasheed IR - Bahar AR IRAD- Department of Medicine, Wayne State University, Detroit, MI, USA. FIR - Bakkannavar, Shankar M IR - Bakkannavar SM IRAD- Department of Forensic Medicine and Toxicology, Manipal Academy of Higher Education, Manipal, India. FIR - Balabekova, Marina IR - Balabekova M IRAD- Department of Pathological Physiology, Kazakh National Medical University, Almaty, Kazakhstan. FIR - Balhara, Yatan Pal Singh IR - Balhara YPS IRAD- National Drug Dependence Treatment Centre, All India Institute of Medical Sciences, New Delhi, India. FIR - Bansal, Hansi IR - Bansal H IRAD- Department of Forensic Science, Government Institute of Forensic Science Nagpur, Nagpur, India. FIR - Bardoe, Dennis IR - Bardoe D IRAD- Department of Public Health Education, University of Skills Training and Entrepreneurial Development, Mampong, Ghana. FIR - Barqawi, Hiba Jawdat IR - Barqawi HJ IRAD- Clinical Sciences Department, University of Sharjah, Sharjah, United Arab Emirates. FIR - Batiha, Abdul-Monim IR - Batiha AM IRAD- Faculty of Nursing, King Khalid University, Mahyil Asir, Saudi Arabia. FIR - Bayleyegn, Nebiyou Simegnew IR - Bayleyegn NS IRAD- Department of Surgery, Jimma University, Jimma, Ethiopia. FIR - Beeraka, Narasimha M IR - Beeraka NM IRAD- Department of Human Anatomy and Histology, I.M. Sechenov First Moscow State Medical University, Moscow, Russia. FIR - Belancic, Andrej IR - Belancic A IRAD- Department of Basic and Clinical Pharmacology and Toxicology, University of Rijeka, Rijeka, Croatia. FIR - Belay, Suleiman Ayalew IR - Belay SA IRAD- Department of Dermatovenereology, University of Gondar, Gondar city, Ethiopia. FIR - Belayneh Yayeh, Melesse IR - Belayneh Yayeh M IRAD- Department of Public Health, Bahir Dar University, Bahir Dar, Ethiopia. FIR - Bente Kamal Tune, Samiun Nazrin IR - Bente Kamal Tune SN IRAD- BRAC James P Grant School of Public Health, BRAC University, Dhaka, Bangladesh. FIR - Bhardwaj, Anuradha IR - Bhardwaj A IRAD- Amity Institute of Public Health and Hospital Administration, Amity University, Noida, India. FIR - Bhardwaj, Pankaj IR - Bhardwaj P IRAD- National Institute for Implementation Research on Non Communicable Diseases, Indian Council of Medical Research, Jodhpur, India. FIR - Bhatnagar, Arushee IR - Bhatnagar A IRAD- Family Medicine Department, Texas Tech University, El Paso, TX, USA. FIR - Bhattacharjee, Priyadarshini IR - Bhattacharjee P IRAD- Translational and Clinical Research Institute, Newcastle University, Newcastle upon Tyne, England. FIR - Bhatti, Gurjit Kaur IR - Bhatti GK IRAD- University Centre for Research and Development, Chandigarh University, Mohali, India. FIR - Bhatti, Rajbir IR - Bhatti R IRAD- Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, India. FIR - Bhuiyan, Md Mehedi Hasan IR - Bhuiyan MMH IRAD- Department of Statistics and Data Science, University of Central Florida, Orlando, FL, USA. FIR - Biniyazova, Aigerim A IR - Biniyazova AA IRAD- Nazarbayev University School of Medicine, Nazarbayev University, Astana, Kazakhstan; Department of National Clinical Practice and Medical Care Organization Improvement, Salidat Kairbekova National Research Center for Health Development, Astana, Kazakhstan. FIR - Bouaoud, Souad IR - Bouaoud S IRAD- Department of Medicine, University Ferhat Abbas of Setif, Setif, Algeria; Department of Epidemiology and Preventive Medicine, University Hospital Saadna Abdenour, Setif, Algeria. FIR - Bryan, Michael Eric IR - Bryan ME IRAD- Nuffield Department of Medicine, University of Oxford, Oxford, England; Cancer Program, Broad Institute of MIT and Harvard, Cambridge, MA, USA. FIR - Buendia, Jefferson Antonio Antonio Buendia IR - Buendia JAAB IRAD- Department of Pharmacology and Toxicology, University of Antioquia, Medellin, Colombia; Warwick Medical School, University of Warwick, Coventry, England. FIR - Busari, Akeem Olayinka IR - Busari AO IRAD- Department of Medical Laboratory Science, Ladoke Akintola University, Ogbomoso, Nigeria. FIR - Bustanji, Yasser IR - Bustanji Y IRAD- School of Pharmacy, The University of Jordan, Amman, Jordan; Department of Basic Biomedical Sciences, University of Sharjah, Sharjah, United Arab Emirates. FIR - Cao, Si IR - Cao S IRAD- Department of Anesthesiology, University at Buffalo, Buffalo, NY, USA. FIR - Carvalho, Andre F IR - Carvalho AF IRAD- IMPInstitute for Mental and Physical Health and Clinical Translation (IMPACT), Deakin University, Geelong, VIC, Australia. FIR - Carvalho, Felix IR - Carvalho F IRAD- Research Unit on Applied Molecular Biosciences (UCIBIO), University of Porto, Porto, Portugal. FIR - Carvalho, Marcia IR - Carvalho M IRAD- Faculty of Health Sciences, University Fernando Pessoa, Porto, Portugal; Associated Laboratory for Green Chemistry (LAQV), University of Porto, Porto, Portugal. FIR - Castaldelli-Maia, Joao Mauricio IR - Castaldelli-Maia JM IRAD- Department of Statistics and Data Science, University of Central Florida, Orlando, FL, USA. FIR - Castaneda-Orjuela, Carlos A IR - Castaneda-Orjuela CA IRAD- Epidemiology and Public Health Evaluation Group, National University of Colombia, Bogota, Colombia. FIR - Chacon-Uscamaita, Pamela Roxana IR - Chacon-Uscamaita PR IRAD- Posgrado De Medicine (Postgraduate Program in Medicine), Universidad Cientifica Del Sur, Lima, Peru. FIR - Chakraborty, Sandip IR - Chakraborty S IRAD- State Disease Investigation Laboratory, Animal Resources Development Department, Agartala, India. FIR - Chander, Subhash IR - Chander S IRAD- Department of Pulmonary and Critical Care Medicine, Mayo Clinic College of Medicine, Rochester, MN, USA; Nephrology and Critical Care Department, Ohio State University, Columbus, OH, USA. FIR - Chandika, Rama Mohan IR - Chandika RM IRAD- Clinical Nutrition Department, Jazan University, Jazan, Saudi Arabia. FIR - Chang, Jung-Chen IR - Chang JC IRAD- College of Medicine, National Taiwan University, Taipei, Taiwan; Department of Nursing, National Taiwan University Hospital, Taipei, Taiwan. FIR - Chattu, Vijay Kumar IR - Chattu VK IRAD- Department of Public Health, Istanbul Medipol University, Istanbul, Turkiye; Department of Community Medicine, Datta Meghe Institute of Medical Sciences, Wardha, India. FIR - Chaudhary, Anis Ahmad IR - Chaudhary AA IRAD- Department of Biology, Imam Mohammad Ibn Saud Islamic University, Riyadh, Saudi Arabia. FIR - Chaudhuri, Sirshendu IR - Chaudhuri S IRAD- ICMR National Institute of Health Research, Dibrugarh, Indian Council of Medical Research, Dibrugarh, India. FIR - Chen, Hui IR - Chen H IRAD- Science and Technology Department, Northern Jiangsu People's Hospital, Yangzhou, China. FIR - Chopra, Deepti IR - Chopra D IRAD- Department of Pharmacology, All India Institute of Medical Sciences, Bilaspur, India. FIR - Chu, Dinh-Toi IR - Chu DT IRAD- The Interdisciplinary Research Group on Biomedicine and Health, VNU International School (VNUIS), Hanoi, Viet Nam; Faculty of Applied Sciences, VNU International School (VNUIS), Hanoi, Viet Nam. FIR - Chukwu, Isaac Sunday IR - Chukwu IS IRAD- Department of Pediatric Surgery, Federal Medical Centre, Umuahia, Nigeria. FIR - Cosma, Claudia IR - Cosma C IRAD- Department of Health Sciences, University of Florence, Florence, Italy. FIR - Cruz-Martins, Natalia IR - Cruz-Martins N IRAD- Life and Health Sciences Research Institute (ICVS), University of Minho, Braga, Portugal; Institute for Research and Innovation in Health (i3S), University of Porto, Porto, Portugal. FIR - Dadras, Omid IR - Dadras O IRAD- Center for Immigrant and Refugee Health, Public Health Institute, Los Angeles, CA, USA. FIR - Dahabiyeh, Lina IR - Dahabiyeh L IRAD- School of Pharmacy, The University of Jordan, Amman, Jordan. FIR - Dahiya, Neha IR - Dahiya N IRAD- Division of Delivery Research/Implementation Research, Indian Council of Medical Research, Delhi, India. FIR - Dai, Xiaochen IR - Dai X IRAD- Institute for Health Metrics and Evaluation, University of Washington, Seattle, WA, USA; Department of Health Metrics Sciences, School of Medicine, University of Washington, Seattle, WA, USA. FIR - D'Amico, Emanuele IR - D'Amico E IRAD- Department of Medical and Surgical Sciences, University of Foggia, Foggia, Italy. FIR - Dang, Anh Kim IR - Dang AK IRAD- Institute for Global Health Innovations, Duy Tan University, Hanoi, Viet Nam. FIR - Danso, Samuel E IR - Danso SE IRAD- Ga East Municipal Hospital, Ghana Health Service, Accra, Ghana. FIR - Darcho, Samuel Demissie IR - Darcho SD IRAD- Department of Public Health, Haramaya University, Harar, Ethiopia. FIR - Das, Saswati IR - Das S IRAD- Department of Biochemistry, Ministry of Health and Welfare, New Delhi, India. FIR - Deekonda, Sindhura IR - Deekonda S IRAD- Department of Pediatrics, Brookdale University Hospital Medical Center, Brooklyn, NY, USA. FIR - Dejen, Eshetu Tesfaye IR - Dejen ET IRAD- Department of Anesthesia, Bahir Dar University, Bahir Dar, Ethiopia. FIR - Delgado-Enciso, Ivan IR - Delgado-Enciso I IRAD- School of Medicine, University of Colima, Colima, Mexico; Department of Research, State Cancerology Institute of Colima, IMSS-BIENESTAR, Colima, Mexico. FIR - Dervisevic, Emina IR - Dervisevic E IRAD- Department of Forensic Medicine, University of Sarajevo, Sarajevo, Bosnia and Herzegovina. FIR - Dervisevic, Muamer IR - Dervisevic M IRAD- Clinic for Pulmonary Diseases and Tuberculosis "Podhrastovi", University Clinical Center in Sarajevo, Sarajevo, Bosnia and Herzegovina. FIR - Devanbu, Vinoth Gnana Chellaiyan IR - Devanbu VGC IRAD- Chettinad Hospital & Research Institute, Chettinad Academy of Research and Education, Chennai, India. FIR - Devasena, Thiyagarajan IR - Devasena T IRAD- Centre for Nanoscience & Technology, Anna University, Chennai, India. FIR - Devegowda, Devananda IR - Devegowda D IRAD- JSS Medical College, Jagadguru Sri Shivarathreeswara Academy of Health Education and Research, Mysuru, India. FIR - Dhali, Arkadeep IR - Dhali A IRAD- Sheffield Teaching Hospital NHS Foundation Trust, Sheffield, UK. FIR - Dhanaraj, Premnath IR - Dhanaraj P IRAD- Department of Biotechnology, Karunya Institute of Technology and Sciences in INDIA, Coimbatore, India. FIR - Dhimal, Meghnath IR - Dhimal M IRAD- Research Department, Nepal Health Research Council, Kathmandu, Nepal; Institute of Occupational, Social and Environmental Medicine, Goethe University, Frankfurt am Main, Germany. FIR - Dhungel, Bibha IR - Dhungel B IRAD- Population Interventions Unit, University of Melbourne, Melbourne, VIC, Australia. FIR - Dias da Silva, Diana IR - Dias da Silva D IRAD- Escola Superior De Saude (Higher School of Health), Instituto Politecnico Do Porto (Polytechnic Institute of Porto), Porto, Portugal; UCIBIO Applied Molecular Biosciences Unit, University of Porto, Porto, Portugal. FIR - Do, Thao Huynh Phuong IR - Do THP IRAD- Department of Medicine, Can Tho University of Medicine and Pharmacy, Can Tho, Viet Nam. FIR - Dohare, Sushil IR - Dohare S IRAD- Department of Public Health, Jazan University, Jazan, Saudi Arabia. FIR - Doku, Paul Narh IR - Doku PN IRAD- Department of Humanities and Social Sciences, Americal University of Ras Al Khaimah, Ras Al Khaimah, United Arab Emirates; School of Nursing and Midwifery, University of Cape Coast, Cape Coast, Ghana. FIR - Doshi, Ojas Prakashbhai IR - Doshi OP IRAD- Independent Consultant, Bridgewater, NJ, USA. FIR - Dowou, Robert Kokou IR - Dowou RK IRAD- Department of Epidemiology and Biostatistics, University of Health and Allied Sciences, Ho, Ghana. FIR - Duraisamy, Senbagam IR - Duraisamy S IRAD- Faculty of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur, India. FIR - Dure, Aster Dure IR - Dure AD IRAD- School of Nursing, Arba Minch University, Arba Minch, Ethiopia. FIR - Efa, Yohannes Tekalegn IR - Efa YT IRAD- School of Public Health, University of Adelaide, Adelaide, SA, Australia. FIR - Ekholuenetale, Michael IR - Ekholuenetale M IRAD- Faculty of Science and Health, University of Portsmouth, Hampshire, UK. FIR - El Bayoumy, Ibrahim Farahat IR - El Bayoumy IF IRAD- Department of Nutrition and Dietetics, University of Health and Allied Sciences, Ho, Ghana; School of Public Health, Texila American University, Guyana, Guyana. FIR - El Sayed, Iman IR - El Sayed I IRAD- Biomedical Informatics and Medical Statistics Department, Alexandria University, Alexandria, Egypt. FIR - Eladl, Mohamed Ahmed IR - Eladl MA IRAD- Department of Basic Medical Sciences, University of Sharjah, Sharjah, United Arab Emirates; Department of Anatomy and Embryology, Mansoura University, Mansoura, Egypt. FIR - Elhadi, Muhammed IR - Elhadi M IRAD- College of Medicine, Korea University, Seoul, South Korea. FIR - Elkannishy, Sherif IR - Elkannishy S IRAD- Department of Biochemistry, Mansoura University, Mansoura, Egypt; Faculty of Applied Health Science, Horus University, New Damietta, Egypt. FIR - Elmeligy, Omar Abdelsadek Abdou IR - Elmeligy OAA IRAD- Pediatric Dentistry and Dental Public Health Department, Alexandria University, Alexandria, Egypt. FIR - Elrggal, Mahmoud E IR - Elrggal ME IRAD- College of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia. FIR - Eltaha, Chadi IR - Eltaha C IRAD- Department of Pediatrics, University of Texas, Dallas, TX, USA. FIR - Eltahawy, Abdelgawad Salah IR - Eltahawy AS IRAD- Faculty of Veterinary Medicine, Damanhour University, Damanhur, Egypt. FIR - Eltaly, Hatem A IR - Eltaly HA IRAD- Department of Emergency Medicine, University of Pittsburgh Medical Center, Steubenville, OH, USA; Faculty of Medicine, Tanta University, Tanta, Egypt. FIR - Emrie, Ambaw Abebaw IR - Emrie AA IRAD- Department of Pediatrics and Child Health Nursing Education and Service, Bahir Dar University, Bahir Dar, Ethiopia. FIR - Esa, Muhammad IR - Esa M IRAD- Drug Delivery System Excellence Center, Prince of Songkla University Hat Yai, Hat Yai, Thailand. FIR - Esomchi, Collins Nduka IR - Esomchi CN IRAD- Department of Human Anatomy, State University of Medical and Applied Sciences, Igbo Eno, Nigeria; Department of Anatomy, Alex Ekwueme Federal University Ndufu Alike Ikwo, Ikwo, Nigeria. FIR - Ezhilarasan, Devaraj IR - Ezhilarasan D IRAD- Department of Pharmacology, Saveetha University, Chennai, India. FIR - Fagbamigbe, Adeniyi Francis IR - Fagbamigbe AF IRAD- Department of Epidemiology and Medical Statistics, University of Ibadan, Ibadan, Nigeria; Research Centre for Healthcare and Community, Coventry University, Coventry, UK. FIR - Faraji, Seyed Nooreddin IR - Faraji SN IRAD- Department of Pathology, Shiraz University of Medical Sciences, Shiraz, Iran. FIR - Farasani, Abdullah IR - Farasani A IRAD- Department of Medical Laboratory Technology, Jazan University, Jazan, Saudi Arabia. FIR - Fareed, Mohammad IR - Fareed M IRAD- College of Medicine, AlMaarefa University, Riyadh, Saudi Arabia. FIR - Faro, Andre IR - Faro A IRAD- Department of Psychology, Federal University of Sergipe, Sao Cristovao, Brazil. FIR - Fawole, Olufunmilayo Ibitola IR - Fawole OI IRAD- Department of Epidemiology and Medical Statistics, University of Ibadan, Ibadan, Nigeria. FIR - Fazylov, Timur IR - Fazylov T IRAD- Department of Health Informatics, Indiana University Indianapolis, Indianapolis, IN, USA. FIR - Fekadu, Ginenus IR - Fekadu G IRAD- Department of Infectious Diseases and Public Health, City University of Hong Kong, Hong Kong, China; Department of Pharmacy, Wollega University, Nekemte, Ethiopia. FIR - Ferrara, Pietro IR - Ferrara P IRAD- Center for Public Health Research, University of Milan Bicocca, Monza, Italy; Laboratory of Public Health, IRCCS Istituto Auxologico Italiano, Milan, Italy. FIR - Filippini, Tommaso IR - Filippini T IRAD- Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena, Italy; School of Public Health, University of California Berkeley, Berkeley, CA, USA. FIR - Fischer, Florian IR - Fischer F IRAD- Institute of Public Health, Charite Universitatsmedizin Berlin (Charite Medical University Berlin), Berlin, Germany. FIR - Fitriana, Ida IR - Fitriana I IRAD- Department of Pharmacology, Gadjah Mada University, Yogyakarta, Indonesia. FIR - Fortuna Rodrigues, Celia IR - Fortuna Rodrigues C IRAD- Research Unit on Applied Molecular Biosciences (UCIBIO), University of Porto, Porto, Portugal; Faculty of Engineering, University of Porto, Porto, Portugal. FIR - Franklin, Richard Charles IR - Franklin RC IRAD- College of Medicine, Dentistry and Public Health, James Cook University, Townsville, QLD, Australia. FIR - Gadanya, Muktar A IR - Gadanya MA IRAD- Department of Community Medicine, Bayero University Kano, Kano, Nigeria; Department of Community Medicine, Aminu Kano Teaching Hospital, Kano, Nigeria. FIR - Gajdacs, Mario IR - Gajdacs M IRAD- Department of Public Health, University of Szeged, Szeged, Hungary. FIR - Galali, Yaseen IR - Galali Y IRAD- Department of Food Technology, Salahaddin University-Erbil, Erbil, Iraq; Department of Nutrition and Dietetics, Cihan University-Erbil, Erbil, Iraq. FIR - Gandhi, Aravind P P IR - Gandhi APP IRAD- Department of Community Medicine and Family Medicine, All India Institute of Medical Sciences, Nagpur, India. FIR - Gautam, Rupesh K IR - Gautam RK IRAD- Amity Institute of Pharmacy, Amity University Uttar Pradesh, Noida, India. FIR - Gebray, Habtamu Mesele IR - Gebray HM IRAD- Department of Internal Medicine, Woldia Comprehensive Specialized Hospital, Woldia, Ethiopia. FIR - Gessess, Shumiye Shiferaw IR - Gessess SS IRAD- Department of Midwifery, Bahir Dar University, Bahir Dar, Ethiopia. FIR - Gete, Kalab Yigermal IR - Gete KY IRAD- College of Medicine and Health Science, Bahir Dar University, Bahir Dar, Ethiopia. FIR - Ghaffoori Kanaan, Manal Hadi IR - Ghaffoori Kanaan MH IRAD- Department of Nursing, Middle Technical University, Baghdad, Iraq. FIR - Gharaibeh, Lobna IR - Gharaibeh L IRAD- Faculty of Pharmacy, Amman Arab University, Amman, Jordan. FIR - Ghashghaee, Ahmad IR - Ghashghaee A IRAD- School of Public Health, Qazvin University of Medical Sciences, Qazvin, Iran. FIR - Ghimire, Sailaja IR - Ghimire S IRAD- Research Department, Nepal Health Research Council, Kathmandu, Nepal. FIR - Gil, Artyom Urievich IR - Gil AU IRAD- Country Office, World Health Organization (WHO), Astana, Kazakhstan. FIR - Gilani, Syed Amir IR - Gilani SA IRAD- Faculty of Allied Health Sciences, The University of Lahore, Lahore, Pakistan; Afro-Asian Institute, Lahore, Pakistan. FIR - Girmay, Alem Abera IR - Girmay AA IRAD- Department of Nursing, Aksum University, Aksum, Ethiopia. FIR - Gohar, Najam IR - Gohar N IRAD- Department of Medicine, Yale University, New Haven, CT, USA. FIR - Gokulu, Gamze IR - Gokulu G IRAD- Department of Pediatrics, Mersin City Training and Research Hospital, Mersin, Turkiye. FIR - Golechha, Mahaveer IR - Golechha M IRAD- Department of Health Systems and Policy Research, Gandhinagar, India. FIR - Grada, Ayman IR - Grada A IRAD- Department of Dermatology, Case Western Reserve University, Cleveland, OH, USA. FIR - Grivna, Michal IR - Grivna M IRAD- Institute of Public Health, United Arab Emirates University, Al Ain, United Arab Emirates; Department of Public Health and Preventive Medicine, Charles University, Prague, Czech Republic. FIR - Guadie, Habtamu Alganeh IR - Guadie HA IRAD- Department of Health Informatics, Bahir Dar University, Bahir Dar, Ethiopia. FIR - Guan, Bin IR - Guan B IRAD- Tianjin Medical University General Hospital, Tianjin Medical University, Tianjin, China; Cheeloo College of Medicine, Shandong University, Jinan, China. FIR - Gunawardane, Damitha Asanga IR - Gunawardane DA IRAD- Department of Community Medicine, University of Peradeniya, Kandy, Sri Lanka. FIR - Gutierrez-Murillo, Roberth Steven IR - Gutierrez-Murillo RS IRAD- Doctoral Program in Biomedical Gerontology, Pontifical Catholic University of Rio Grande Do Sul, Porto Alegre, Brazil. FIR - Gutu, Yalo Daba IR - Gutu YD IRAD- College of Health and Medical Science, Haramaya University, Harar, Ethiopia. FIR - Hadi, Najah R IR - Hadi NR IRAD- Department of Clinical Pharmacology and Medicine, University of Kufa, Najaf, Iraq. FIR - Hagins, Hailey IR - Hagins H IRAD- Institute for Health Metrics and Evaluation, University of Washington, Seattle, WA, USA. FIR - Hajibeygi, Ramtin IR - Hajibeygi R IRAD- Department of Radiology, Tehran University of Medical Sciences, Tehran, Iran. FIR - Halder, Pritam IR - Halder P IRAD- Department of Community Medicine, Post Graduate Institute of Medical Education and Research, Chandigarh, India; Centre for Community Medicine, All India Institute of Medical Sciences, New Delhi, India. FIR - Hamad, Islam M IR - Hamad IM IRAD- Department of Pharmacy, American University of Madaba, Amman, Jordan. FIR - Hamidi, Samer IR - Hamidi S IRAD- School of Health and Environmental Studies, Hamdan Bin Mohammed Smart University, Dubai, United Arab Emirates. FIR - Harsini, Sara IR - Harsini S IRAD- Department of Medical Imaging, University of Toronto, Toronto, ON, Canada. FIR - Has, Eka Mishbahatul Marah IR - Has EMM IRAD- Department of Advanced Nursing, Universitas Airlangga (Airlangga University), Surabaya, Indonesia; School of Nursing and Midwivery, La Trobe University, Bundoora, VIC, Australia. FIR - Hasaballah, Ahmed I IR - Hasaballah AI IRAD- Department of Zoology and Entomology, Al-Azhar University, Cairo, Egypt. FIR - Hasan, Abd Alhadi IR - Hasan AA IRAD- Nursing Program, Fakeeh College for Medical Sciences, Jeddah, Saudi Arabia. FIR - Hashem Zadeh, Arezou IR - Hashem Zadeh A IRAD- Institute of Radiology and Radiological Sciences, Tehran University of Medical Sciences, Tehran, Iran; Department of Surgery, University of California San Francisco, Oakland, MD, USA. FIR - Hashempur, Mohammad Hashem IR - Hashempur MH IRAD- Research Center for Traditional Medicine and History of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. FIR - Hasnain, Md Saquib IR - Hasnain MS IRAD- Centre for Promotion of Research, Graphic Era Deemed University, Dehradun, India. FIR - Hassan, Abbas M IR - Hassan AM IRAD- Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA. FIR - Hassan, Ibrahim Nagmeldin IR - Hassan IN IRAD- Department of Medicine, University of Khartoum, Khartoum, Sudan. FIR - Hassan, Md Imtaiyaz IR - Hassan MI IRAD- Centre for Interdisciplinary Research in Basic Sciences, Jamia Millia Islamia, New Delhi, India. FIR - Hassan, Nageeb IR - Hassan N IRAD- Ajman University, Ajman, United Arab Emirates. FIR - Hassanian-Moghaddam, Hossein IR - Hassanian-Moghaddam H IRAD- Social Determinants of Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Chapter of Addiction Medicine, University of Sydney, Sydney, NSW, Australia. FIR - Heibati, Behzad IR - Heibati B IRAD- Department of Medicine, University of Alberta, Edmonton, AB, Canada. FIR - Heidari, Mohammad IR - Heidari M IRAD- Community-Oriented Nursing Midwifery Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran. FIR - Hein, Zaw Myo IR - Hein ZM IRAD- College of Medicine, Ajman University, Ajman, United Arab Emirates. FIR - Holla, Ramesh IR - Holla R IRAD- Kasturba Medical College Mangalore, Manipal Academy of Higher Education, Manipal, India. FIR - Hossain, Md Sabbir IR - Hossain MS IRAD- Department of Statistics, Shahjalal University of Science and Technology, Sylhet, Bangladesh. FIR - Hostiuc, Sorin IR - Hostiuc S IRAD- Department of Legal Medicine and Bioethics, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania; Department of Clinical Legal Medicine, National Institute of Legal Medicine Mina Minovici, Bucharest, Romania. FIR - Hu, Guoqing IR - Hu G IRAD- Department of Epidemiology and Health Statistics, Central South University, Changsha, China. FIR - Hu, Mengsi IR - Hu M IRAD- Shandong Provincial Hospital, Shandong First Medical University, Jinan, China; Cheeloo College of Medicine, Shandong First Medical University, Jinan, China. FIR - Huang, Junjie IR - Huang J IRAD- Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. FIR - Huda, Mega Hasanul IR - Huda MH IRAD- Pediatric Nursing Department, University of Indonesia, Depok, Indonesia. FIR - Hussain, Javid IR - Hussain J IRAD- Department of Biological Sciences and Chemistry (DBSC), University of Nizwa, Nizwa, Oman. FIR - Ibitoye, Segun Emmanuel IR - Ibitoye SE IRAD- Department of Health Promotion and Education, University of Ibadan, Ibadan, Nigeria. FIR - Ibrayeva, Anel IR - Ibrayeva A IRAD- Scientific and Technological Park, Kazakh National Medical University, Almaty, Kazakhstan. FIR - Ilesanmi, Olayinka Stephen IR - Ilesanmi OS IRAD- Department of Accounting, Finance, Healthcare Administration, Information Systems & Business Analytics, Fayetteville State University, Fayetteville, NC, USA. FIR - Ilic, Irena M IR - Ilic IM IRAD- Faculty of Medicine, University of Belgrade, Belgrade, Serbia. FIR - Ilic, Milena D IR - Ilic MD IRAD- Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia. FIR - Immurana, Mustapha IR - Immurana M IRAD- Institute of Health Research, University of Health and Allied Sciences, Ho, Ghana. FIR - Imoh, Lucius Chidiebere IR - Imoh LC IRAD- Department of Chemical Pathology, University of Jos, Jos, Nigeria; Department of Chemical Pathology, Jos University Teaching Hospital, Jos, Nigeria. FIR - Irham, Lalu Muhammad IR - Irham LM IRAD- Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta, Indonesia. FIR - Ishaqui, Azfar Athar IR - Ishaqui AA IRAD- Department of Clinical Pharmacy, King Khalid University, Abha, Saudi Arabia. FIR - Islam, Md Shahinul IR - Islam MS IRAD- Department of Public Health, International University of Business Agriculture and Technology (IUBAT), Dhaka, Bangladesh. FIR - Ismoldayev, Yerlan IR - Ismoldayev Y IRAD- Department of Urology, Kazakh National Medical University, Almaty, Kazakhstan. FIR - Ispayeva, Zhanat Bakhitovna IR - Ispayeva ZB IRAD- Department of Allergology, Kazakh National Medical University, Almaty, Kazakhstan. FIR - Iwu-Jaja, Chinwe Juliana IR - Iwu-Jaja CJ IRAD- Department of Global Health, South African Medical Research Council, Cape Town, South Africa; Department of Global Health, Stellenbosch University, Cape Town, South Africa. FIR - J, Kezia Angeline IR - J KA IRAD- SRM School of Public Health, Sri Ramaswamy Memorial Institute of Science and Technology, Chennai, India. FIR - Jacob, Jaison IR - Jacob J IRAD- College of Nursing, All India Institute of Medical Sciences, Bhubaneswar, India. FIR - Jahanshahi, Ali IR - Jahanshahi A IRAD- Cancer Program, Broad Institute of MIT and Harvard, Cambridge, MA, USA. FIR - Jahrami, Haitham IR - Jahrami H IRAD- College of Medicine and Health Sciences, Arabian Gulf University, Manama, Bahrain; Government Hospital, Manama, Bahrain. FIR - Jain, Sonal IR - Jain S IRAD- School of Medicine, Johns Hopkins University, Baltimore, MD, USA. FIR - Jakovljevic, Mihajlo IR - Jakovljevic M IRAD- Section of Economic & Social Sciences, Humanities & Arts, The World Academy of Sciences UNESCO-TWAS, Trieste, Italy; Shaanxi University of Technology, Hanzhong, China. FIR - Jamal, Armaan IR - Jamal A IRAD- Department of Public Health Sciences, University of Chicago, Chicago, IL, USA. FIR - Jamaluddin, Jazlan IR - Jamaluddin J IRAD- Department of Primary Care Medicine, Universiti Malaya, Kuala Lumpur, Malaysia. FIR - James, Jerin IR - James J IRAD- Department of Medicine, Wayne State University, Detroit, MI, USA. FIR - Jarrahi, Esmaeil IR - Jarrahi E IRAD- Department of Stem Cell and Developmental Biology, Royan Institute, Tehran, Iran. FIR - Jayatilleke, Achala Upendra IR - Jayatilleke AU IRAD- Postgraduate Institute of Medicine, University of Colombo, Colombo, Sri Lanka; Faculty of Graduate Studies, Institute for Violence and Injury Prevention, Colombo, Sri Lanka. FIR - Jeon, Jae Joon IR - Jeon JJ IRAD- Department of Dermatology, Yonsei University Wonju College of Medicine, Wonju, South Korea; Department of Pulmonary and Critical Care Medicine, Mayo Clinic College of Medicine, Rochester, MN, USA. FIR - Jeong, Gwang Hun IR - Jeong GH IRAD- Division of Public Health Sciences, Washington University in St. Louis, St. Louis, MO, USA; Department of Public Health Sciences, Seoul National University, Seoul, South Korea. FIR - Jeong, Seogsong IR - Jeong S IRAD- Department of Biomedical Informatics, Korea University, Seoul, South Korea. FIR - Jimoh, Muideen Adewale IR - Jimoh MA IRAD- Medical Laboratory Science/Prosthetics and Orthotics, Federal University of Health Sciences Ila-Orangun Osun State, Ila-Orangun, Nigeria. FIR - Joseph, Akaninyene Paul IR - Joseph AP IRAD- School of Biological Sciences, Queen's University, Belfast, Northern Ireland. FIR - Joseph, Alex IR - Joseph A IRAD- SRM School of Public Health, Sri Ramaswamy Memorial Institute of Science and Technology, Kattankulathur, India. FIR - Joseph, Nitin IR - Joseph N IRAD- Department of Community Medicine, Manipal Academy of Higher Education, Mangalore, India. FIR - Joshi, Krupal IR - Joshi K IRAD- Institute of National Importance, All India insitute of medical sciences, Rajkot, India. FIR - Joshua, Charity Ehimwenma IR - Joshua CE IRAD- Department of Economics, National Open University, Benin City, Nigeria. FIR - Kabir, Zubair IR - Kabir Z IRAD- Research Department, TobaccoFree Research Institute Ireland, Dublin, Ireland; School of Public Health, University College Cork, Cork, Ireland. FIR - Kadir, Dler Hussein IR - Kadir DH IRAD- Department of Statistics, Salahaddin University-Erbil, Erbil, Iraq; Department of Business Administration, Cihan University-Erbil, Erbil, Iraq. FIR - Kakkar, Ashish Kumar IR - Kakkar AK IRAD- Department of Pharmacology, Post Graduate Institute of Medical Education and Research, Chandigarh, India. FIR - Kamath, Ashwin IR - Kamath A IRAD- Kasturba Medical College Mangalore, Manipal Academy of Higher Education, Manipal, India. FIR - Kamenova, Saltanat IR - Kamenova S IRAD- Brody School of Medicine, East Carolina University, Greenville, NC, USA. FIR - Kamorudeen, Ramat T IR - Kamorudeen RT IRAD- Elderwood Rehabilitation Clinical Centre, Buffalo, NY, USA; Department of Public Health, South Wales University, Treforest, UK. FIR - Kang, Jiseung IR - Kang J IRAD- School of Health and Environmental Science, Korea University, Seoul, South Korea; Department of Anesthesia, Critical Care and Pain Medicine, Massachusetts General Hospital, Boston, MA, USA. FIR - Kanmodi, Kehinde Kazeem IR - Kanmodi KK IRAD- Office of the Executive Director, Cephas Health Research Initiative Inc, Ibadan, Nigeria; Department of Public Health, Thomas Adewumi University, Oko, Nigeria. FIR - Kapoor, Neeti IR - Kapoor N IRAD- Department of Forensic Science, Government Institute of Forensic Science Nagpur, Nagpur, India. FIR - Karkhah, Samad IR - Karkhah S IRAD- Independent Consultant, Bridgewater, NJ, USA. FIR - Karpinski, Tomasz M IR - Karpinski TM IRAD- Chair and Department of Medical Microbiology, Poznan University of Medical Sciences, Poznan, Poland. FIR - Kassa, Gebrehiwot G IR - Kassa GG IRAD- Department of Biomedical Sciences, Aksum University, Aksum, Ethiopia. FIR - Kavei, Yasin IR - Kavei Y IRAD- Department of Medicine, Tehran University of Medical Sciences, Tehran, Iran. FIR - Khader, Yousef Saleh IR - Khader YS IRAD- Department of Public Health, Jordan University of Science and Technology, Irbid, Jordan. FIR - Khan, Ajmal IR - Khan A IRAD- Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, Oman. FIR - Khan, Mohammad Aasif IR - Khan MA IRAD- Department of Radiation Oncology, University of Texas Health Science Center at San Antonio, San Antonio, TX, USA. FIR - Khan, Muhammad Arslan IR - Khan MA IRAD- Department of Pharmacy, Minhaj University Lahore, Lahore, Pakistan. FIR - Khan, Muhammad Umer IR - Khan MU IRAD- Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Lahore, Pakistan. FIR - Khan, Yusuf Saleem IR - Khan YS IRAD- College of Medicine, University of Hail, Hail, Saudi Arabia. FIR - Khan, Zahid IR - Khan Z IRAD- Department of Cardiology, University of South Wales, Treforest, UK; Department of Cardiology, University of Buckingham, Buckingham, UK. FIR - Khasbage, Sameer Uttamaro IR - Khasbage SU IRAD- Department of Pharmacology, All India Institute of Medical Sciences, Raipur, India. FIR - Khokhar, Manoj IR - Khokhar M IRAD- Department of Biochemistry, All India Institute of Medical Sciences, Jodhpur, India. FIR - Kifle, Zemene Demelash IR - Kifle ZD IRAD- Department of Pharmacology, University of Gondar, Gondar, Ethiopia. FIR - Kini B, Sanjay IR - Kini B S IRAD- Department of Immunology, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA. FIR - Kisa, Adnan IR - Kisa A IRAD- School of Health Sciences, Kristiania University College, Oslo, Norway; Department of International Health and Sustainable Development, Tulane University, New Orleans, LA, USA. FIR - Kishore, Ladli IR - Kishore L IRAD- School of Pharmaceutical Sciences, CGC University, Mohali, Mohali, India. FIR - Koh, David S Q IR - Koh DSQ IRAD- Saw Swee Hock School of Public Health, National University of Singapore, Singapore, Singapore; Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan. FIR - Kolahi, Ali-Asghar IR - Kolahi AA IRAD- Social Determinants of Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. FIR - Kondybayeva, Aida IR - Kondybayeva A IRAD- Scientific and Educational Center for Neurology and Applied Neuroscience, Kazakh National Medical University, Almaty, Kazakhstan. FIR - Koren, Gerbrand IR - Koren G IRAD- Copernicus Institute of Sustainable Development, Utrecht University, Utrecht, Netherlands. FIR - Koul, Archana IR - Koul A IRAD- Amity Institute of Public Health and Hospital Administration, Amity University, Noida, India. FIR - Kozhumam, Arthi S IR - Kozhumam AS IRAD- Feinberg School of Medicine, Northwestern University, Chicago, IL, USA. FIR - Krishan, Kewal IR - Krishan K IRAD- Nephrology and Critical Care Department, Ohio State University, Columbus, OH, USA. FIR - Krishna, Varun IR - Krishna V IRAD- Department of Forensic Medicine and Toxicology, Pondicherry University, Puducherry, India. FIR - Kuan, Garry IR - Kuan G IRAD- School of Health Sciences, Universiti Sains Malaysia, Kubang Kerian, Malaysia; School of Physical Education, Henan University, Keifeng, China. FIR - Kuddus, Mohammed IR - Kuddus M IRAD- Department of Biochemistry, University of Hail, Hail, Saudi Arabia. FIR - Kumar, Ashwini IR - Kumar A IRAD- Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, India. FIR - Kumar, Kamal IR - Kumar K IRAD- Department of Medical Imaging, University of Toronto, Toronto, ON, Canada. FIR - Kumar, Manoj IR - Kumar M IRAD- Department of Microbiology, Lady Hardinge Medical College, New Delhi, India. FIR - Kumar, Mukesh IR - Kumar M IRAD- Department of Community Medicine, Vardhman Mahavir Medical College and Safdarjung Hospital, Delhi, India. FIR - Kumar, Narendar IR - Kumar N IRAD- Department of Pharmacy Practice, University of Sindh, Jamshoro, Pakistan. FIR - Kumar, Nitesh IR - Kumar N IRAD- Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research, Hajipur, Hajipur, India. FIR - Kumar, Radha IR - Kumar R IRAD- Department of Paediatrics, Sri Ramaswamy Memorial Institute of Science and Technology, Chennai, India. FIR - Kusuma, Dian IR - Kusuma D IRAD- Department of Public Health and Epidemiology, Khalifa University of Science and Technology, Abu Dhabi, United Arab Emirates; Faculty of Public Health, University of Indonesia, Depok, Indonesia. FIR - Kuttybayev, Assylkhan IR - Kuttybayev A IRAD- Department of Public Health, Kazakh National Medical University, Almaty, Kazakhstan. FIR - Kwak, Taeho IR - Kwak T IRAD- Department of Biomedical Informatics, Korea University, Seoul, South Korea. FIR - Kyto, Ville IR - Kyto V IRAD- Clinical Research Center, Turku University Hospital, Turku, Finland; Heart Center, University of Turku, Turku, Finland. FIR - Laflamme, Lucie IR - Laflamme L IRAD- Department of Global Public Health, Karolinska Institute, Stockholm, Sweden; Institute for Social and Health Sciences, University of South Africa, Pretoria, South Africa. FIR - Lahariya, Chandrakant IR - Lahariya C IRAD- Division of Evidence Synthesis, Foundation for People-centric Health Systems, New Delhi, India; Division of Lifestyle Medicine, Centre for Health: the Specialty Practice, New Delhi, India. FIR - Lajunen, Timo IR - Lajunen T IRAD- Department of Psychology, University of Helsinki, Helsinki, Finland; Department of Psychology, Norwegian University of Science and Technology, Trondheim, Norway. FIR - Lantos, Tamas IR - Lantos T IRAD- Department of Medical Physics and Informatics, University of Szeged, Szeged, Hungary. FIR - Le, Long Khanh-Dao IR - Le LK IRAD- Health Economics Division, Monash University, Melbourne, VIC, Australia. FIR - Ledda, Caterina IR - Ledda C IRAD- Department of Clinical and Experimental Medicine, University of Catania, Catania, Italy. FIR - Lee, Hwamin IR - Lee H IRAD- Department of Biomedical Informatics, Korea University, Seoul, South Korea. FIR - Lee, Seung Won IR - Lee SW IRAD- Department of Precision Medicine, Sungkyunkwan University, Suwon-si, South Korea. FIR - Lee, Wei-Chen IR - Lee WC IRAD- Department of Family Medicine, University of Texas Medical Branch, Galveston, TX, USA. FIR - Li, Ming-Chieh IR - Li MC IRAD- Department of Health Promotion and Health Education, National Taiwan Normal University, Taipei, Taiwan. FIR - Liu, Xuefeng IR - Liu X IRAD- Department of Quantitative Health Sciences, Cleveland Clinic, Cleveland, OH, USA; Department of Medicine, Case Western Reserve University, Cleveland, OH, USA. FIR - Llanaj, Erand IR - Llanaj E IRAD- Department of General Practice and Family Medicine and Translational and Health Services Research, Bielefeld University, Bielefeld, Germany. FIR - Lytvyak, Ellina IR - Lytvyak E IRAD- Department of Medicine, University of Alberta, Edmonton, AB, Canada; Tulane School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA, USA. FIR - M Amin, Hawraz Ibrahim IR - M Amin HI IRAD- Faculty of Pharmacy, Tishk International University, Erbil, Iraq; Department of Pharmaceutical Science, University of Eastern Piedmont, Novara, Italy. FIR - Ma, Zheng Feei IR - Ma ZF IRAD- Centre for Public Health and Wellbeing, University of the West of England, Bristol, England. FIR - Madfa, Ahmed A IR - Madfa AA IRAD- Restorative Dental Science, University of Hail, Hail, Saudi Arabia. FIR - Magwe, Edward Augustine IR - Magwe EA IRAD- Department of Pharmaceutical Sciences, Ruaha Catholic University (RUCU), Iringa, Tanzania. FIR - Mahadeshwara Prasad, D R IR - Mahadeshwara Prasad DR IRAD- Department of Forensic Medicine & Toxicology, Mysore Medical College & Research Institute, Mysooru, India; Department of Health & Family Welfare, Government of Karnataka, Bangalore, India. FIR - Mahmood, Nozad Hussein IR - Mahmood NH IRAD- Cihan University-Sulaimaniya, Sulaymaniyah, Iraq. FIR - Malik, Tabarak IR - Malik T IRAD- Institute of Health, Jimma University, Jimma, Ethiopia; Division of Research & Development, Lovely Professional University, Phagwara, India. FIR - Manjani, Lokesh IR - Manjani L IRAD- Internal Medicine Department, MedStar Health, Washington, DC, USA. FIR - Marrapodi, Maria Maddalena IR - Marrapodi MM IRAD- Department of Woman, Child and General and Specialist Surgery, University of Campania 'Luigi Vanvitelli', Naples, Italy. FIR - Marzo, Roy Rillera IR - Marzo RR IRAD- Faculty of Humanities and Health Sciences, Curtin University, Sarawak, Malaysia; College of Health Sciences, Abu Dhabi University, Abu Dhabi, United Arab Emirates. FIR - Marzouk, Sammer IR - Marzouk S IRAD- Medical Scientist Training Program, Northwestern University, Chicago, IL, USA. FIR - Massahi, Sharareh IR - Massahi S IRAD- Economics Department, University of Oklahoma, Norman, OK, USA. FIR - Mathangasinghe, Yasith IR - Mathangasinghe Y IRAD- Department of Anatomy and Developmental Biology, Monash University, Clayton, VIC, Australia; Department of Anatomy, Genetics and Biomedical Informatics, University of Colombo, Colombo, Sri Lanka. FIR - Mathur, Neeta IR - Mathur N IRAD- Department of Community Medicine, Apollo Institute of Medical Sciences and Research, Hyderabad, India. FIR - McKee, Martin IR - McKee M IRAD- Department of Health Services Research and Policy, London School of Hygiene & Tropical Medicine, London, England. FIR - Meena, Jitendra IR - Meena J IRAD- Department of Pediatrics, All India Institute of Medical Sciences, New Delhi, India. FIR - Mekonnen, Lord Tadesse IR - Mekonnen LT IRAD- Women's and Children Health Department, Uppsala University, Uppsala, Sweden; Department of Medicine, Bethel Medical College, Addis Ababa, Ethiopia. FIR - Menezes, Godfred Antony IR - Menezes GA IRAD- Department of Medical Microbiology and Immunology, Trinity Medical Sciences University, St. Vincent, Saint Vincent and the Grenadines; Kasturba Medical College, Manipal Academy of Higher Education, Mangalore, India. FIR - Menezes, Ritesh G IR - Menezes RG IRAD- Department of Pathology, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia. FIR - Mengist, Mahider Ayalew Ayalew IR - Mengist MAA IRAD- Asrat Woldeyes Health Science Campus, Debre Berhan University, Debre Birhan, Ethiopia. FIR - Mesfin, Rahwa Adisu IR - Mesfin RA IRAD- Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, USA. FIR - Mestrovic, Tomislav IR - Mestrovic T IRAD- Institute for Health Metrics and Evaluation, University of Washington, Seattle, WA, USA; University Centre Varazdin, University North, Varazdin, Croatia. FIR - Mettananda, Chamila Dinushi Kukulege IR - Mettananda CDK IRAD- Department of Pharmacology, University of Kelaniya, Ragama, Sri Lanka; Clinical Medicine Department, Colombo North Teaching Hospital, Ragama, Sri Lanka. FIR - Mettananda, Sachith IR - Mettananda S IRAD- Department of Paediatrics, University of Kelaniya, Ragama, Sri Lanka; University Paediatrics Unit, Colombo North Teaching Hospital, Ragama, Sri Lanka. FIR - Metwally, Mohamed M M IR - Metwally MMM IRAD- Department of Pathology Faculty of Veterinary Medicine Zagazig University, Zagazig University, Zagazig, Egypt. FIR - Miazgowski, Bartosz IR - Miazgowski B IRAD- Clinical Emergency Department, Pomeranian Medical University, Szczecin, Poland; Pomeranian Medical University, Szczecin, Poland. FIR - Miller, Ted R IR - Miller TR IRAD- College of Human Medicine, Michigan State University, Flint, MI, USA. FIR - Ming, Long Chiau IR - Ming LC IRAD- School of Pharmacy, Sunway University, Sunway City, Malaysia. FIR - Mittal, Chaitanya IR - Mittal C IRAD- Department of Forensic Medicine and Toxicology, All India Institute of Medical Sciences, Patna, India. FIR - Mohamed, Nouh Saad IR - Mohamed NS IRAD- Molecular Biology Unit, Sirius Training and Research Centre, Khartoum, Sudan; Bio-Statistical and Molecular Biology Department, Sirius Training and Research Centre, Khartoum, Sudan. FIR - Mohamed Ahmed, Khabab Abbasher Hussien IR - Mohamed Ahmed KAH IRAD- Faculty of Medicine, University of Khartoum, Khartoum, Sudan; Department of Pathophysiology, St. George's University, St.George's, Grenada. FIR - Mohammad, Taj IR - Mohammad T IRAD- Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India; Centre for Interdisciplinary Research in Basic Sciences (CIRBSc), Jamia Millia Islamia, New Delhi, India. FIR - Mohammed, Al-Kassim Hassan IR - Mohammed AH IRAD- Graduate School of Health Sciences, Ankara University, Ankara, Turkiye; Faculty of Pharmaceutical Sciences, Bayero University Kano, Kano, Nigeria. FIR - Mokdad, Ali H IR - Mokdad AH IRAD- Institute for Health Metrics and Evaluation, University of Washington, Seattle, WA, USA; Department of Health Metrics Sciences, School of Medicine, University of Washington, Seattle, WA, USA. FIR - Molinaro, Sabrina IR - Molinaro S IRAD- Institute of Clinical Physiology, National Research Council, Pisa, Italy. FIR - Moni, Mohammad Ali IR - Moni MA IRAD- AI & Cyber Futures Institute, Charles Sturt University, Bathurst, NSW, Australia; The University of Queensland, Brisbane, QLD, Australia. FIR - Mosalamiaghili, Seyedarad IR - Mosalamiaghili S IRAD- International Joint Center, Acibadem Mehmet Ali Aydinlar University, Istanbul, Turkiye. FIR - Motappa, Rohith IR - Motappa R IRAD- Department of Community Medicine, Manipal Academy of Higher Education, Mangalore, India. FIR - Mozahheb Yousefi, Kimia IR - Mozahheb Yousefi K IRAD- Antimicrobial Resistance Research Center, Iran University of Medical Sciences, Tehran, Iran; Hazrat-e Rasool General Hospital, Iran University of Medical Sciences, Tehran, Iran. FIR - Mubarik, Sumaira IR - Mubarik S IRAD- Unit of Pharmacotherapy, Epidemiology and Economics, University of Groningen (Rijksuniversiteit Groningen), Groningen, Netherlands; Department of Epidemiology and Biostatistics, Wuhan University, Wuhan, China. FIR - Mulita, Francesk IR - Mulita F IRAD- Department of Surgery, General University Hospital of Patras, Patras, Greece; Faculty of Medicine, University of Thessaly, Larissa, Greece. FIR - Murray, Christopher J L IR - Murray CJL IRAD- Institute for Health Metrics and Evaluation, University of Washington, Seattle, WA, USA; Department of Health Metrics Sciences, School of Medicine, University of Washington, Seattle, WA, USA. FIR - Musoke, David IR - Musoke D IRAD- School of Public Health, Makerere University, Kampala, Uganda. FIR - Mustafa, Ghulam IR - Mustafa G IRAD- College of Medicine, Shaqra University, Shaqra, Saudi Arabia; Department of Pediatrics & Pediatric Pulmonology, Institute of Mother & Child Care, Multan, Pakistan. FIR - Mustafa, Sherzad Ibrahim IR - Mustafa SI IRAD- Department of Pathology and Microbiology, Duhok University, Duhok, Iraq. FIR - Nagarajan, Ahamarshan Jayaraman IR - Nagarajan AJ IRAD- Research and Analytics Department, Initiative for Financing Health and Human Development, Chennai, India; Department of Research and Analytics, Bioinsilico Technologies, Chennai, India. FIR - Nainu, Firzan IR - Nainu F IRAD- Faculty of Pharmacy, Hasanuddin University, Makassar, Indonesia. FIR - Naqvi, Syed Zeeshan Haider Zeeshan Haider IR - Naqvi SZHZH IRAD- Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Lahore, Pakistan. FIR - Nayak, Biswa Prakash IR - Nayak BP IRAD- Amity Institute of Forensic Sciences, Amity University, Noida, India. FIR - Nayon, Md Fahad Shahariar IR - Nayon MFS IRAD- School of Public Health, University of Adelaide, Adelaide, SA, Australia. FIR - Negoi, Ionut IR - Negoi I IRAD- Department of General Surgery, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania; Department of Epidemiology and Biostatistics, University of South Carolina, Columbia, SC, USA. FIR - Nepal, Samata IR - Nepal S IRAD- Department of Community Medicine, Lumbini Medical College, Palpa, Nepal. FIR - Netsere, Henok Biresaw IR - Netsere HB IRAD- School of Nursing, University of Gondar, Gondar, Ethiopia; College of Medicine and Health Sciences, Bahir Dar University, Bahir Dar, Ethiopia. FIR - Nguyen, Cuong Tat IR - Nguyen CT IRAD- Institute for Global Health Innovations, Duy Tan University, Da Nang, Viet Nam. FIR - Nguyen, Duy Cao IR - Nguyen DC IRAD- Institute for Global Health Innovations, Duy Tan University, Da Nang, Viet Nam. FIR - Nguyen, Hau Thi Hien IR - Nguyen HTH IRAD- Faculty of Medicine, Duy Tan University, Da Nang, Viet Nam; Institute for Research and Training in Medicine, Biology and Pharmacy, Duy Tan University, Da Nang, Viet Nam. FIR - Nguyen, Hien Thu IR - Nguyen HT IRAD- Institute for Global Health Innovations, Duy Tan University, Da Nang, Viet Nam. FIR - Nguyen, Huong Lan Thi IR - Nguyen HLT IRAD- Institute for Global Health Innovations, Duy Tan University, Da Nang, Viet Nam. FIR - Nguyen, Trang IR - Nguyen T IRAD- Faculty of Medicine, Duy Tan University, Da Nang, Viet Nam; Faculty of Public Health, VNU University of Medicine and Pharmacy, Hanoi, Viet Nam. FIR - Ngwa Ayanji, Ngum Milca IR - Ngwa Ayanji NM IRAD- Department of Microbiology and Parasitology Faculty of Science, University of Buea, Buea, Cameroon. FIR - Niranjan, Vikram IR - Niranjan V IRAD- Health Research Institute, University of Limerick, Limerick, Ireland. FIR - Nri-Ezedi, Chisom Adaobi IR - Nri-Ezedi CA IRAD- Department of Paediatrics, Nnamdi Azikiwe University, Awka, Nigeria. FIR - Odetokun, Ismail A IR - Odetokun IA IRAD- Department of Veterinary Public Health and Preventive Medicine, University of Ilorin, Ilorin, Nigeria. FIR - Ojasanya, Rasaq A IR - Ojasanya RA IRAD- Department of Population Medicine, University of Guelph, Guelph, ON, Canada. FIR - Okeke, Sylvester Reuben IR - Okeke SR IRAD- Centre for Social Research in Health, University of New South Wales, Sydney, NSW, Australia; University of Sydney, Sydney, NSW, Australia. FIR - Okoro, Lenz Nwachinemere IR - Okoro LN IRAD- Department of Community Medicine, David Umahi Federal University Teaching Hospital, Uburu, Nigeria; Department of Community Medicine, David Umahi Federal University of Health Sciences, Uburu, Nigeria. FIR - Olagunju, Andrew T IR - Olagunju AT IRAD- Department of Psychiatry and Behavioral Neurosciences, McMaster University, Hamilton, ON, Canada; Department of Psychiatry, University of Lagos, Lagos, Nigeria. FIR - Oluwafemi, Yinka Doris IR - Oluwafemi YD IRAD- Department of Microbiology, University of Medical Sciences, Ondo, Ondo, Nigeria. FIR - Omar Bali, Ahmed IR - Omar Bali A IRAD- Institute of Research and Development, Duy Tan University, Da Nang, Viet Nam; Jadara Research Center, Jadara University, Irbid, Jordan. FIR - Ordak, Michal IR - Ordak M IRAD- Centre of Regenerative Medicine, Medical University of Bialystok, Bialystok, Poland. FIR - Orru, Hans IR - Orru H IRAD- Institute of Family Medicine and Public Health, University of Tartu, Tartu, Estonia; Section of Sustainable Health, Umea University, Umea, Sweden. FIR - Ortiz-Prado, Esteban IR - Ortiz-Prado E IRAD- One Health Research Group, Universidad De Las Americas, Quito, Ecuador. FIR - Osborne, Augustus IR - Osborne A IRAD- Department of Biological Sciences, Njala University, Freetown, Sierra Leone. FIR - Otorkpa, Chinenye Oche IR - Otorkpa CO IRAD- Health Services, Federal University Lokoja, Kogi, Lojoka Kogi State, Nigeria. FIR - Otorkpa, Oche Joseph IR - Otorkpa OJ IRAD- School of Public Health, Texila American University, Georgetown, Guyana. FIR - P A, Mahesh IR - P A M IRAD- Department of Respiratory Medicine, Jagadguru Sri Shivarathreeswara Academy of Health Education and Research, Mysore, India. FIR - Padubidri, Jagadish Rao IR - Padubidri JR IRAD- Department of Forensic Medicine and Toxicology, Manipal Academy of Higher Education, Mangalore, India. FIR - Panda, Sujogya Kumar IR - Panda SK IRAD- Centre for Biotechnology, Siksha 'O' Anusandhan (Deemed to Be University), Bhubaneswar, India. FIR - Pandey, Ashok IR - Pandey A IRAD- Research Department, Policy Research Institute, Kathmandu, Nepal; Research Department, Public Health Research Society Nepal, Kathmandu, Nepal. FIR - Papadimopoulos, Ilias IR - Papadimopoulos I IRAD- Department of Medicine and Surgery, University of Bologna, Bologna, Italy; Medical University of Vienna, Vienna, Austria. FIR - Parida, Amrita IR - Parida A IRAD- Department of Pharmacology, Manipal Academy of Higher Education, Manipal, India. FIR - Parikh, Romil R IR - Parikh RR IRAD- Division of Health Policy and Management, University of Minnesota, Minneapolis, MN, USA. FIR - Parmar, Arpit IR - Parmar A IRAD- College of Graduate Health Sciences, University of Tennessee, Memphis, TN, USA. FIR - Patel, Meet Navinkumar IR - Patel MN IRAD- Department of Medicine, Government Medical College Surat, Surat, India. FIR - Patel, Mitesh IR - Patel M IRAD- Department of Computer Science and Bioscience, Marwadi University, Rajkot, India. FIR - Patel, Neel Navinkumar IR - Patel NN IRAD- Department of Cardiovascular Medicine, University of Tennessee, Nashville, TN, USA. FIR - Patel, Satyananda IR - Patel S IRAD- Zoology Section, Regional Institute of Education (RIE), Ajmer, India. FIR - Patil, Ashlesh IR - Patil A IRAD- Department of Physiology, All India Institute of Medical Sciences, Nagpur, India. FIR - Patil, Shankargouda IR - Patil S IRAD- College of Dental Medicine, Roseman University of Health Sciences, South Jordan, UT, USA. FIR - Patoulias, Dimitrios IR - Patoulias D IRAD- Second Propedeutic Department of Internal Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece. FIR - Pattnaik, Snigdha IR - Pattnaik S IRAD- Department of Community Medicine, Malla Reddy Medical College for Women, Hyderabad, Hyderabad, India. FIR - Peden, Amy E IR - Peden AE IRAD- School of Population Health, University of New South Wales, Kensington, NSW, Australia; Kate Marimon School of Public Health, The University of Texas at San Antonio, San Antonio, TX, USA. FIR - Perna, Simone IR - Perna S IRAD- Department of Food, Environmental and Nutritional Sciences, University of Milan, Milan, Italy. FIR - Peter, Roshni Mary IR - Peter RM IRAD- Department of Community Medicine, Sri Ramaswamy Memorial Institute of Science and Technology, Kattankulathur, India. FIR - Phillips, Michael R IR - Phillips MR IRAD- Shanghai Mental Health Center, Shanghai Jiao Tong University, Shanghai, China; Department of Psychiatry, Columbia University, New York, NY, USA. FIR - Piracha, Zahra Zahid IR - Piracha ZZ IRAD- Center for Research Development, University of Cyberjaya, Cyberjaya, Malaysia; International Center of Medical Sciences Research (ICMSR), Islamabad, Pakistan. FIR - Pirera, Edoardo IR - Pirera E IRAD- Department of Promoting Health, Maternal-Infant, Excellence and Internal and Specialized Medicine (PROMISE) G. D'Alessandro, University of Palermo, Palermo, Italy. FIR - Piyasena, Mapa M Prabhath N IR - Piyasena MMPN IRAD- Vision and Eye Research Institute, Anglia Ruskin University, Cambridge, England. FIR - Porntaveetus, Thantrira IR - Porntaveetus T IRAD- Center of Excellence in Precision Medicine and Digital Health, Chulalongkorn University, Bangkok, Thailand. FIR - Pourasghary, Sajjad IR - Pourasghary S IRAD- Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. FIR - Pourbabaki, Reza IR - Pourbabaki R IRAD- Department of Occupational Health Engineering, Sirjan School of Medical Sciences, Sirjan, Iran. FIR - Priya, Harsh IR - Priya H IRAD- Centre for Dental Education and Research, All India Institute of Medical Sciences, New Delhi, India. FIR - Puri, Pooja IR - Puri P IRAD- Amity Institute of Forensic Sciences, Amity University, Noida, India. FIR - Qanash, Husam IR - Qanash H IRAD- Faculty of Business and Law, University of Portsmouth, Portsmouth, UK; Medical and Diagnostic Research Center, University of Hail, Hail, Saudi Arabia. FIR - Qattea, Ibrahim IR - Qattea I IRAD- Department of Neonatology, Case Western Reserve University, Akron, OH, USA. FIR - Radhakrishnan, Venkatraman IR - Radhakrishnan V IRAD- Department of Medical Oncology, Cancer Institute (W.I.A), Chennai, India. FIR - Rahim, Fakher IR - Rahim F IRAD- Department of Medical Biology, Kocaeli University, Izmit, Turkiye; Internal Medical Faculty, Osh State University, Osh, Kyrgyzstan. FIR - Rahimi, Sajjad IR - Rahimi S IRAD- Department of Environmental Health Engineering, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran; Health Science Research Centre, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran. FIR - Rahman, Firman Suryadi IR - Rahman FS IRAD- Department of Public Health, Universitas Airlangga (Airlangga University), Surabaya, Indonesia. FIR - Rahman, Mohammad Hifz Ur IR - Rahman MHU IRAD- College of Dental Medicine, Roseman University of Health Sciences, South Jordan, UT, USA. FIR - Rahman, Mosiur IR - Rahman M IRAD- Department of Population Science and Human Resource Development, University of Rajshahi, Rajshahi, Bangladesh. FIR - Rahmoune, Hakim IR - Rahmoune H IRAD- Faculty of Medicine, University of Setif Algeria, Setif, Algeria; LIRSSEI Research Lab, University of Setif Algeria, Setif, Algeria. FIR - Raj, Jeffrey Pradeep IR - Raj JP IRAD- Division of Clinical Pharmacology, Manipal Academy of Higher Education, Manipal, India. FIR - Raja, Adarsh IR - Raja A IRAD- Department of Cardiology, Dow University of Health Sciences, Karachi, Pakistan. FIR - Rajendran, Gunaseelan IR - Rajendran G IRAD- Trauma and Emergency Medicine Department, All India Institute of Medical Sciences (AIIMS), Madurai, India. FIR - Rajendran, Judah IR - Rajendran J IRAD- Department of Cardiovascular Medicine, Cleveland Clinic, Cleveland, OH, USA. FIR - Rakhymbayev, Nurgali Amanbaiuly IR - Rakhymbayev NA IRAD- Faculty of Pharmacy, JSC "South Kazakhstan Medical Academy", Shymkent, Kazakhstan. FIR - Ramadan, Mahmoud Mohammed IR - Ramadan MM IRAD- Department of Clinical Sciences, University of Sharjah, Sharjah, United Arab Emirates; Department of Cardiology, Mansoura University, Mansoura, Egypt. FIR - Ramamoorthy, Lakshmi IR - Ramamoorthy L IRAD- College of Nursing, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, India. FIR - Ramasamy, Shakthi Kumaran IR - Ramasamy SK IRAD- Department of Radiology, Stanford University, Stanford, CA, USA. FIR - Ramazanu, Sheena IR - Ramazanu S IRAD- Saw Swee Hock School of Public Health, National University of Singapore, Singapore, Singapore; School of Nursing & Health Sciences, Hong Kong Metropolitan University, Hong Kong, China. FIR - Ramezani, Zahra IR - Ramezani Z IRAD- Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran. FIR - Rani, Smitha IR - Rani S IRAD- Department of Forensic Medicine and Toxicology, Jagadguru Sri Shivarathreeswara University, Mysore, India. FIR - Rao, Rajath IR - Rao R IRAD- Department of Community Medicine, ESIC Medical College, Bengaluru, India. FIR - Rao, Sowmya J IR - Rao SJ IRAD- Department of Oral Pathology, Microbiology and Forensic Odontology, Sharavathi Dental College and Hospital, Shimogga, India. FIR - Rathish, Devarajan IR - Rathish D IRAD- Department of Family Medicine, Rajarata University of Sri Lanka, Anuradhapura, Sri Lanka. FIR - Rathore, Jigyasa IR - Rathore J IRAD- Amity Institute of Public Health and Hospital Administration, Amity University, Uttar Pradesh, Uttar Pradesh, India. FIR - Ravi, Lokesh IR - Ravi L IRAD- Centre for Digital Health and Precision Medicine, The Apollo University, Chittoor, India. FIR - Ravi, Renju IR - Ravi R IRAD- Department of Basic Medical Sciences, Jazan University, Jazan, Saudi Arabia. FIR - Rawaf, Salman IR - Rawaf S IRAD- Department of Primary Care and Public Health, Imperial College London, London, England; Academic Public Health England, Public Health England, London, England. FIR - Rehman, Abdul IR - Rehman A IRAD- Department of Pathological Sciences, Ajman University, Ajman, United Arab Emirates. FIR - Rezaei, Nazila IR - Rezaei N IRAD- Non-communicable Diseases Research Center, Tehran University of Medical Sciences, Tehran, Iran. FIR - Rhee, Taeho Gregory IR - Rhee TG IRAD- Department of Public Health Sciences, University of Connecticut, Farmington, CT, USA; Department of Psychiatry, Yale University, New Haven, CT, USA. FIR - Rout, Himanshu Sekhar IR - Rout HS IRAD- Department of Analytical and Applied Economics, Utkal University, Bhubaneswar, India; RUSA Centre of Excellence in Public Policy and Governance, Utkal University, Bhubaneswar, India. FIR - Saad, Aly M A IR - Saad AMA IRAD- Cardiovascular Department, Zagazig University, Zagazig, Egypt. FIR - Saad, Khaled IR - Saad K IRAD- Faculty of Medicine, Assiut University, Assiut, Egypt. FIR - Saddik, Basema Ahmad IR - Saddik BA IRAD- Department of Public Health and Epidemiology, Khalifa University, Abu Dhabi, United Arab Emirates; School of Arts and Social Sciences, Edith Cowan University, Bunbury, WA, Australia. FIR - Sadee, Bashdar Abuzed IR - Sadee BA IRAD- Department of Food Technology, Salahaddin University-Erbil, Erbil, Iraq; Department of Nutrition and Dietetics, Cihan University-Erbil, Erbil, Iraq. FIR - Sadek, Bassem IR - Sadek B IRAD- Department of Pharmacology and Therapeutics, United Arab Emirates University, Al Ain, United Arab Emirates. FIR - Sadek, Mustafa IR - Sadek M IRAD- Faculty of Veterinary Medicine, Qena University, Qena city, Egypt; Department of Hygiene and Food Control, King Salman International University, Ras sidr, Egypt. FIR - Saeb, Mohammad Reza IR - Saeb MR IRAD- Department of Pharmaceutical Chemistry, International Medical University, Gdansk, Poland. FIR - Saeed, Mohd IR - Saeed M IRAD- Department of Biology, University of Hail, Hail, Saudi Arabia. FIR - Saeed, Umar IR - Saeed U IRAD- Szechenyi Istvan University, Gyor, Hungary; Irfan Suat Gunsel Operational Research Institute, Near East University, Nicosia, Turkiye. FIR - Safari, Mehdi IR - Safari M IRAD- Research Institute for Health Sciences and Environment, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Zilber College of Public Health, University of Wisconsin Milwaukee, Milwaukee, WI, USA. FIR - Saha, Indranil IR - Saha I IRAD- Department of Epidemiology, Indian Council of Medical Research, Kolkata, India. FIR - Saheb Sharif-Askari, Narjes IR - Saheb Sharif-Askari N IRAD- Clinical Sciences Department, University of Sharjah, Sharjah, United Arab Emirates. FIR - Saif, Zahra IR - Saif Z IRAD- Department of Psychiatry, Ministry of Health, Manama, Bahrain. FIR - Sajadi, S Mohammad IR - Sajadi SM IRAD- College of Pharmacy, Al-Hadba University, Mosul, Iraq. FIR - Sakshaug, Joseph W IR - Sakshaug JW IRAD- LMU-Munich, Munich, Germany; Institute for Employment Research, Nuremberg, Germany. FIR - Salami, Afeez Abolarinwa IR - Salami AA IRAD- Cephas Health Research Initiative Inc, Ibadan, Nigeria; Department of Oral and Maxillofacial Surgery, University College Hospital, Ibadan, Ibadan, Nigeria. FIR - Saleh, Mohamed A IR - Saleh MA IRAD- College of Medicine, University of Sharjah, Sharjah, United Arab Emirates; Faculty of Pharmacy, Mansoura University, Mansoura, Egypt. FIR - Samodra, Yoseph Leonardo IR - Samodra YL IRAD- Research Center for Environmental Changes, Academia Sinica, Taipei, Taiwan; Benang Merah Research Center (BMRC), Minahasa Utara, Indonesia. FIR - Samy, Abdallah M IR - Samy AM IRAD- Department of Entomology, Ain Shams University, Cairo, Egypt; Medical Ain Shams Research Institute (MASRI), Ain Shams University, Cairo, Egypt. FIR - Sanjeev, Rama Krishna IR - Sanjeev RK IRAD- Department of Pediatrics, SRM Medical College Hospital and Research Centre, Kattankulathur, India. FIR - Sarmadi, Mohammad IR - Sarmadi M IRAD- Faculty of Science, Queensland University of Technology, Brisbane, QLD, Australia; Health Sciences Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran. FIR - Sarmiento-Suarez, Rodrigo IR - Sarmiento-Suarez R IRAD- College of Health Sciences, University of Wyoming, Laramie, WY, USA. FIR - Sathya Narayanan, Mukesh Kumar IR - Sathya Narayanan MK IRAD- Department of Epidemiology, National Institute for Research in Tuberculosis, Chennai, India. FIR - Schmidtke, Tim IR - Schmidtke T IRAD- School of Arts and Social Sciences, Edith Cowan University, Bunbury, WA, Australia. FIR - Schwebel, David C C IR - Schwebel DCC IRAD- Office of the Vice President for Research, University of Iowa, Iowa City, IA, USA. FIR - Selvaraj, Siddharthan IR - Selvaraj S IRAD- Faculty of Dentistry, University of Puthisastra, Phnom Penh, Cambodia. FIR - Semreen, Mohammad H IR - Semreen MH IRAD- College of Pharmacy, University of Sharjah, Sharjah, United Arab Emirates; Research Institute of Medical & Health Sciences, University of Sharjah, Sharjah, United Arab Emirates. FIR - Senthilkumaran, Subramanian IR - Senthilkumaran S IRAD- Emergency Department, Manian Medical Centre, Erode, India. FIR - Sethi, Yashendra IR - Sethi Y IRAD- Department of Medicine, Swami Vivekanand Subharti University, Meerut, India. FIR - Seyed Alshohadaei, Seyed Mohammad IR - Seyed Alshohadaei SM IRAD- Department of Anesthesiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran. FIR - Shahid, Samiah IR - Shahid S IRAD- Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Lahore, Pakistan; Research Centre for Health Sciences (RCHS), The University of Lahore, Lahore, Pakistan. FIR - Shahrahmani, Fatemeh IR - Shahrahmani F IRAD- Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. FIR - Shahwan, Moyad Jamal IR - Shahwan MJ IRAD- Center for Medical and Bio-Allied Health Sciences Research, Ajman University, Ajman, United Arab Emirates. FIR - Shaikh, Masood Ali IR - Shaikh MA IRAD- Independent Consultant, Karachi, Pakistan. FIR - Shamim, Muhammad Aaqib IR - Shamim MA IRAD- Department of Pharmacology, All India Institute of Medical Sciences, Jodhpur, India. FIR - Shan, Dan IR - Shan D IRAD- Lancaster University, Lancaster, England; Columbia University, New York, NY, USA. FIR - Shanawaz, Mohd IR - Shanawaz M IRAD- College of Nursing and Health Sciences, Jazan University, Jazan, Saudi Arabia. FIR - Shannawaz, Mohammed IR - Shannawaz M IRAD- Amity Institute of Public Health and Hospital Administration, Amity University, Noida, India. FIR - Sharangi, Amit Baran IR - Sharangi AB IRAD- Department of Plantation Spices Medicinal and Aromatic Crops, Bidhan Chandra Krishi Viswavidyalaya Agricultural University in India, Mohanpur, India; School of Agriculture, Food and Ecosystem Sciences, University of Melbourne, Burnley, VIC, Australia. FIR - Sharifan, Amin IR - Sharifan A IRAD- Department for Evidence-based Medicine and Evaluation, University for Continuing Education Krems, Krems, Austria. FIR - Sharifi Rad, Javad IR - Sharifi Rad J IRAD- Universidad Espiritu Santo, Samborondon, Ecuador. FIR - Sharma, Avimanu IR - Sharma A IRAD- Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur, India. FIR - Sharma, Bhoopesh Kumar IR - Sharma BK IRAD- Department of Forensic Science, Shree Guru Gobind Singh Tricentenary University, Gurugram, India. FIR - Sharma, Bunty IR - Sharma B IRAD- Department of Biotechnology, Graphic Era (Deemed to Be University), Dehradun, India. FIR - Sharma, Kamal IR - Sharma K IRAD- UN Mehta Institute of Cardiology and Research Center, B.J. Medical College, Ahmedabad, India; Department of Cardiology, Government Medical College, Ahmedabad, India. FIR - Sharma, Kamlesh IR - Sharma K IRAD- Division of Physical Sciences, Karunya Institute of Technology and Sciences, Tamil Nadu, India. FIR - Sharma, Vishal IR - Sharma V IRAD- Department of Forensic Science, Panjab University, Chandigarh, India. FIR - Shashamo, Bereket Beyene IR - Shashamo BB IRAD- Department of Nursing, Arba Minch University, Arba Minch, Ethiopia. FIR - Shastry, Shamee IR - Shastry S IRAD- Kasturba Medical College, Manipal Academy of Higher Education, Manipal, India. FIR - Sheidaei, Ali IR - Sheidaei A IRAD- Department of Environmental Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA. FIR - Shen, Jiabin IR - Shen J IRAD- Psychology Department, University of Massachusetts Lowell, Boston, MA, USA. FIR - Sherchan, Samendra P IR - Sherchan SP IRAD- Department of Biology, Morgan State University, Baltimore, MD, USA; Department of Environmental Health Sciences, Tulane University, New Orleans, LA, USA. FIR - Shetty, B Suresh Kumar IR - Shetty BSK IRAD- Department of Forensic Medicine and Toxicology, Manipal Academy of Higher Education, Mangalore, India. FIR - Shetty, Mahabalesh IR - Shetty M IRAD- Alva's Institute of Medical Sciences & Research Centre, Rajiv Gandhi University of Health Sciences, Moodubidire, India. FIR - Shetty, Pavanchand IR - Shetty P IRAD- KS Hegde Medical Academy, Nitte University, Mangalore, India. FIR - Shetty, Suraj S IR - Shetty SS IRAD- KS Hegde Medical Academy, Nitte University, Mangalore, India. FIR - Shirali, Arun IR - Shirali A IRAD- Department of General Medicine, Manipal Academy of Higher Education, Manipal, India. FIR - Shirazi, Shirindokht IR - Shirazi S IRAD- Department of Orthodontics, Boston University, Boston, MA, USA. FIR - Shitaye, Nebiyu Aniley IR - Shitaye NA IRAD- Department of Surgery, Bahir Dar University, Bahir Dar, Ethiopia. FIR - Shittu, Aminu IR - Shittu A IRAD- Department of Veterinary Public Health and Preventive Medicine, Usmanu Danfodiyo University, Sokoto, Sokoto, Nigeria. FIR - Shivarov, Velizar IR - Shivarov V IRAD- Department of Experimental Research, Medical University Pleven, Pleven, Bulgaria; Department of Genetics, Sofia University "St. Kliment Ohridiski", Sofia, Bulgaria. FIR - Shrestha, Sunil IR - Shrestha S IRAD- Nepal Health Research and Innovation Foundation, Lalitpur, Nepal; Person-Centered Research Directorate, Monash University, Box Hill, VIC, Australia. FIR - Sidamo, Negussie Boti IR - Sidamo NB IRAD- School of Public Health, Arba Minch University, Arba Minch, Ethiopia. FIR - Siddique, Muhammad Irfan IR - Siddique MI IRAD- College of Pharmacy, Northern Border University, Rafha, Saudi Arabia. FIR - Singh, Harmanjit IR - Singh H IRAD- Department of Pharmacology, Government Medical College and Hospital, Chandigarh, India. FIR - Singh, Jawahar IR - Singh J IRAD- Department of Psychiatry, All India Institute of Medical Sciences, Bathinda, India. FIR - Singh, Paramdeep IR - Singh P IRAD- Department of Radiodiagnosis, All India Institute of Medical Sciences, Bathinda, India. FIR - Singh, Poornima Suryanath IR - Singh PS IRAD- Amity Institute of Public Health and Hospital Administration, Amity University, Noida, India. FIR - Sinto, Robert IR - Sinto R IRAD- Department of Internal Medicine, University of Indonesia, Jakarta, Indonesia; Department of Internal Medicine, Dr. Cipto Mangunkusumo National Hospital, Jakarta Pusat, Indonesia. FIR - Skryabin, Valentin Yurievich IR - Skryabin VY IRAD- Books Committee, Royal College of Psychiatrists, London, UK. FIR - Skryabina, Anna Aleksandrovna IR - Skryabina AA IRAD- Department of Infectious Diseases and Epidemiology, Pirogov Russian National Research Medical University, Moscow, Russia. FIR - Sobia, Farrukh IR - Sobia F IRAD- Department of Public Health, Jazan University, Jazan, Saudi Arabia. FIR - Song, Yizhe IR - Song Y IRAD- Department of Medicine, Washington University in St. Louis, St. Louis, MO, USA. FIR - Spagnolo, Anna Maria IR - Spagnolo AM IRAD- Department of Health Sciences, University of Genoa, Genoa, Italy. FIR - Sree, Lokasani Vijaya IR - Sree LV IRAD- Department of Health, A.C.S. Medical College and Hospital, Hyderabad, India. FIR - Sridharan, Kannan IR - Sridharan K IRAD- Department of Pharmacology and Therapeutics, Arabian Gulf University, Manama, Bahrain. FIR - Stanikzai, Muhammad Haroon IR - Stanikzai MH IRAD- Department of Public Health, Kandahar University, Kandahar, Afghanistan. FIR - Stergachis, Andy IR - Stergachis A IRAD- Department of Health Metrics Sciences, School of Medicine, University of Washington, Seattle, WA, USA; Department of Pharmacy, University of Washington, Seattle, WA, USA. FIR - Stokes, Mark A IR - Stokes MA IRAD- Department of Psychology, Deakin University, Burwood, VIC, Australia. FIR - Straube, Sebastian IR - Straube S IRAD- Department of Medicine, University of Alberta, Edmonton, AB, Canada; School of Public Health, University of Alberta, Edmonton, AB, Canada. FIR - Su, Chen-Yang IR - Su CY IRAD- McGill University, Montreal, QC, Canada. FIR - Subramanian, Balachandran IR - Subramanian B IRAD- Department of Physiology, Saveetha University, Chennai Tamil Nadu, India. FIR - Subramaniyan, Vetriselvan IR - Subramaniyan V IRAD- Department of Medical Sciences, Sunway University, Subang Jaya, Malaysia. FIR - Subramaniyan Natarajan, Meenakshi Sundari IR - Subramaniyan Natarajan MS IRAD- Department of General Medicine, Sri Ramaswamy Memorial Institute of Science and Technology, Chengalpattu, India. FIR - Sullman, Mark J M IR - Sullman MJM IRAD- Department of Social Sciences, University of Nicosia, Nicosia, Cyprus; Department of Life and Health Sciences, University of Nicosia, Nicosia, Cyprus. FIR - Swain, Chandan Kumar IR - Swain CK IRAD- Department of Analytical and Applied Economics, Utkal University, Bhubaneswar, India. FIR - Szarpak, Lukasz IR - Szarpak L IRAD- Institute of Medical Sciences, The John Paul II Catholic University of Lublin, Lublin, Poland. FIR - Tabatabaei, Seyyed Mohammad IR - Tabatabaei SM IRAD- Department of Medical Informatics, Mashhad University of Medical Sciences, Mashhad, Iran; Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. FIR - Tabibi, Ramin IR - Tabibi R IRAD- Department of Health, Safety, and Environmental Management, Abadan University of Medical Sciences, Abadan, Iran. FIR - Tabish, Mohammad IR - Tabish M IRAD- College of Medicine, Shaqra University, Shaqra, Saudi Arabia. FIR - Tahavvori, Amir IR - Tahavvori A IRAD- Feinstein Institutes for Medical Research, Urmia University of Medical Sciences, Manhasset, NY, USA. FIR - Taheri, Niloofar IR - Taheri N IRAD- Department of Psychiatry and Behavioral Sciences, Stanford University, Palo Alto, CA, USA. FIR - Taheri Abkenar, Yasaman IR - Taheri Abkenar Y IRAD- Living Systems Institute, University of Exeter, Exeter, England. FIR - Tampa, Mircea IR - Tampa M IRAD- Department of Dermatology, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania; Department of Dermato-Venereology, Dr. Victor Babes Clinical Hospital of Infectious Diseases and Tropical Diseases, Bucharest, Romania. FIR - Tan, Ching Siang IR - Tan CS IRAD- School of Pharmacy, KPJ Healthcare University, Negeri Seremban, Malaysia; Department of Pharmacy Practice, Universitas Airlangga (Airlangga University), Surabaya, Indonesia. FIR - Tangiisuran, Balamurugan IR - Tangiisuran B IRAD- School of Pharmaceutical Sciences, University of Science Malaysia, Gelugor, Malaysia; School of Pharmaceutical Sciences, University of Science Malaysia, Penang, Malaysia. FIR - Tazhiyeva, Aigul Yelgondiyevna IR - Tazhiyeva AY IRAD- Department of Science, Kazakh National Medical University, Almaty, Kazakhstan. FIR - Temsah, Mohamad-Hani IR - Temsah MH IRAD- College of Medicine, Alfaisal University, Riyadh, Saudi Arabia; Pediatric Intensive Care Unit, King Saud University, Riyadh, Saudi Arabia. FIR - Tesfu, Azimeraw Arega IR - Tesfu AA IRAD- Department of Midwifery, Bahir Dar University, Bahir Dar, Ethiopia. FIR - Thangavelu, Lakshmi IR - Thangavelu L IRAD- University of Cyberjaya, Selangor, Malaysia. FIR - Thapar, Rekha IR - Thapar R IRAD- Department of Community Medicine, Manipal Academy of Higher Education, Mangalore, India. FIR - Ticoalu, Jansje Henny Vera IR - Ticoalu JHV IRAD- Faculty of Public Health, Universitas Sam Ratulangi (Sam Ratulangi University), Manado, Indonesia. FIR - Tleshev, Madi IR - Tleshev M IRAD- Department of Prosthetic Dentistry, Kazakh National Medical University, Almaty, Kazakhstan. FIR - Tran, Thang Huu IR - Tran TH IRAD- Department of Internal Medicine, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Viet Nam; Department of Business Analytics, University of Massachusetts Dartmouth, Dartmouth, MA, USA. FIR - Trico, Domenico IR - Trico D IRAD- Department of Clinical and Experimental Medicine, University of Pisa, Pisa, Italy. FIR - Tse, Gary IR - Tse G IRAD- Tianjin Key Laboratory of Ionic-Molecular Function of Cardiovascular Disease, Second Hospital of Tianjin Medical University, Tianjin, China. FIR - Ullah, Atta IR - Ullah A IRAD- Natural and Medical Sciences Research Center, University of Nizwa, Nizwa, Oman. FIR - Ullah, Riaz IR - Ullah R IRAD- Department of Pharmacognosy, King Saud University, Riyadh, Saudi Arabia. FIR - Umapathi, Krishna Kishore IR - Umapathi KK IRAD- Department of Pediatric Cardiology, West Virginia University, Charleston, WV, USA. FIR - Umar, Lawan IR - Umar L IRAD- Department of Physiotherapy, Federal Medical Centre, Gusau, Nigeria. FIR - Unnikrishnan, Bhaskaran IR - Unnikrishnan B IRAD- Kasturba Medical College Mangalore, Manipal Academy of Higher Education, Manipal, India. FIR - Upadhyay, Era IR - Upadhyay E IRAD- Amity Institute of Biotechnology, Amity University Rajasthan, Jaipur, India. FIR - Usman, Jibrin Sammani IR - Usman JS IRAD- Department of Physiotherapy, Bayero University Kano, Kano, Nigeria; Department of Rehabilitation Sciences, Hong Kong Polytechnic University, Hong Kong, China. FIR - Uz Zaman, Sameer IR - Uz Zaman S IRAD- Faculty of Medical Sciences, Newcastle University Medicine Malaysia, Iskandar Puteri, Malaysia; Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, England. FIR - Uzuncibuk, Hande IR - Uzuncibuk H IRAD- Department of Orthodontics, Trakya University, Edirne, Turkiye. FIR - V R, Rakesh IR - V R R IRAD- College of Nursing, All India Institute of Medical Sciences, Nagpur, India. FIR - Vaithinathan, Asokan Govindaraj IR - Vaithinathan AG IRAD- College of Health and Sport Sciences, University of Bahrain, Zallaq, Bahrain. FIR - Verma, Akshaya Kumar IR - Verma AK IRAD- Siksha 'O' Anusandhan (Deemed to Be University), Bhubaneswar, India. FIR - Vinh, Tuan IR - Vinh T IRAD- Radcliffe Department of Medicine, University of Oxford, Oxford, England. FIR - Violante, Francesco S IR - Violante FS IRAD- Department of Medical and Surgical Sciences, University of Bologna, Bologna, Italy; Occupational Medicine Unit, Sant'Orsola Malpighi Hospital, Bologna, Italy. FIR - Vu, Linh IR - Vu L IRAD- Faculty of Public Health, VNU University of Medicine and Pharmacy, Hanoi, Viet Nam; International Institute for Training and Research (INSTAR), VNU University of Medicine and Pharmacy, Hanoi, Viet Nam. FIR - Wada, Abubakar Sadiq IR - Wada AS IRAD- Department of Pharmacology and Therapeutics, Bayero University Kano, Kano, Nigeria. FIR - Waheed, Yasir IR - Waheed Y IRAD- Irfan Suat Gunsel Operational Research Institute, Near East University, Nicosia, Turkiye; VIZJA University, Warsaw, Poland. FIR - Walia, Megha IR - Walia M IRAD- Department of Forensic Science, Shree Guru Gobind Singh Tricentenary University, Gurugram, India. FIR - Wang, Wei IR - Wang W IRAD- School of Public Health, Xuzhou Medical University, Xuzhou, China. FIR - Wang, Yanzhong IR - Wang Y IRAD- School of Life Course and Population Sciences, King's College London, London, England. FIR - Wang, Yuan-Pang IR - Wang YP IRAD- Department of Statistics and Data Science, University of Central Florida, Orlando, FL, USA. FIR - Weerakoon, Kosala Gayan IR - Weerakoon KG IRAD- Department of Parasitology, Rajarata University of Sri Lanka, Anuradhapura, Sri Lanka. FIR - Weldemedhn, Shimels Getaneh IR - Weldemedhn SG IRAD- Department of Emergency and Critical Care Medicine, Debre Berhan University, Debre berhan, Ethiopia. FIR - Wickramasinghe, Nuwan Darshana IR - Wickramasinghe ND IRAD- Department of Community Medicine, Rajarata University of Sri Lanka, Anuradhapura, Sri Lanka. FIR - Williams, Faustine IR - Williams F IRAD- Independent Investigator, Washington, DC, USA. FIR - Winarni, Sri IR - Winarni S IRAD- Faculty of Public Health, Diponegoro University, Kota Semarang, Indonesia. FIR - Wonde, Tewodros Eshete IR - Wonde TE IRAD- Faculty of Health, University of Technology Sydney, Sydney, NSW, Australia. FIR - Yadav, Vikas IR - Yadav V IRAD- Department of Environmental Health and Epidemiology, National Institute for Research in Environmental Health, Bhopal, India. FIR - Yang, Xinxin IR - Yang X IRAD- School of Public Health, Xuzhou Medical University, Xuzhou, China. FIR - Ye, Pengpeng IR - Ye P IRAD- National Center for Chronic and Noncommunicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China; The George Institute for Global Health, University of New South Wales, Sydney, NSW, Australia. FIR - Yesodharan, Renjulal IR - Yesodharan R IRAD- Manipal College of Nursing, Manipal Academy of Higher Education, Udupi, India. FIR - Yigit, Arzu IR - Yigit A IRAD- Department of Health Management, Suleyman Demirel Universitesi (Suleyman Demirel University), Isparta, Turkiye. FIR - Yisak, Etsehiwot H IR - Yisak EH IRAD- School of Public Health, Washington University in St. Louis, St. Louis, MO, USA. FIR - Yon, Dong Keon IR - Yon DK IRAD- Department of Pediatrics, Kyung Hee University, Seoul, South Korea. FIR - Yonemoto, Naohiro IR - Yonemoto N IRAD- Department of Biostatistics, University of Toyama, Toyama, Japan; Department of Public Health, Juntendo University, Tokyo, Japan. FIR - Yu, Yong IR - Yu Y IRAD- School of Public Health, Hubei University of Medicine, Shiyan, China. FIR - Zanghi, Aurora IR - Zanghi A IRAD- Department of Medical and Surgical Sciences, University of Foggia, Foggia, Italy. FIR - Zastrozhin, Michael IR - Zastrozhin M IRAD- Department of Bioengineering and Therapeutical Sciences, University of California San Francisco, San Francisco, CA, USA; Department of Administration, PGxAI, San Francisco, CA, USA. FIR - Zeariya, Mohammed G M IR - Zeariya MGM IRAD- Department of Public Health, University of Hail, Hail, Saudi Arabia; Department of Zoology and Entomology, Al-Azhar University, Cairo, Egypt. FIR - Zeru, Eyael M IR - Zeru EM IRAD- Department of Psychiatry, Johns Hopkins University, Baltimore, MD, USA. FIR - Zhanuzakov, Murat IR - Zhanuzakov M IRAD- Faculty of Medicine and Healthcare, Al Farabi Kazakh National University, Almaty, Kazakhstan. FIR - Zhong, Claire Chenwen IR - Zhong CC IRAD- Jockey Club School of Public Health and Primary Care, The Chinese University of Hong Kong, Hong Kong, China. FIR - Zhumagaliuly, Abzal IR - Zhumagaliuly A IRAD- Atchabarov Scientific Research Institute of Fundamental and Applied Medicine, Kazakh National Medical University, Almaty, Kazakhstan. FIR - Zrieq, Rafat Mohammad IR - Zrieq RM IRAD- Department of Nursing Administration, University of Hail, Hail, Saudi Arabia. FIR - Zyoud, Ahed H IR - Zyoud AH IRAD- Department of Chemistry, An-Najah National University, Nablus, Palestine. FIR - Zyoud, Sa'ed H IR - Zyoud SH IRAD- Department of Clinical and Community Pharmacy, An-Najah National University, Nablus, Palestine; Clinical Research Centre, An-Najah National University Hospital, Nablus, Palestine. FIR - Zyoud, Shaher H IR - Zyoud SH IRAD- Department of Building Engineering and Environment, Palestine Technical University (Kadoorie), Tulkarem, Palestine; Civil Engineering and Sustainable Structures, Palestine Technical University (Kadoorie), Tulkarem, Palestine. FIR - Hay, Simon I IR - Hay SI IRAD- Institute for Health Metrics and Evaluation, University of Washington, Seattle, WA, USA; Department of Health Metrics Sciences, School of Medicine, University of Washington, Seattle, WA, USA. FIR - Ong, Kanyin Liane IR - Ong KL IRAD- Institute for Health Metrics and Evaluation, University of Washington, Seattle, WA, USA. FIR - Moberg, Madeline E IR - Moberg ME IRAD- Institute for Health Metrics and Evaluation, University of Washington, Seattle, WA, USA. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:52 PHST- 2026/03/16 00:00 [received] PHST- 2026/07/28 00:00 [revised] PHST- 2026/08/05 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:52 [entrez] AID - S2468-2667(26)00173-8 [pii] AID - 10.1016/S2468-2667(26)00173-8 [doi] PST - aheadofprint SO - Lancet Public Health. 2026 Oct 27:S2468-2667(26)00173-8. doi: 10.1016/S2468-2667(26)00173-8. PMID- 42945979 OWN - NLM STAT- Publisher LR - 20261027 IS - 1474-5488 (Electronic) IS - 1470-2045 (Linking) DP - 2026 Oct 27 TI - Pazopanib alternating with bevacizumab in patients with metastatic clear-cell renal cell carcinoma naive to treatment: a multicentre, single-arm, phase 1/2 trial. LID - S1470-2045(26)00394-3 [pii] LID - 10.1016/S1470-2045(26)00394-3 [doi] AB - BACKGROUND: VEGFR tyrosine kinase inhibitors (TKIs) are considered the backbone for the treatment of metastatic clear-cell renal cell carcinoma. We hypothesise that the administration of bevacizumab during scheduled breaks in pazopanib (TKI) treatment can improve tolerability and prolong progression-free survival compared with historical controls. METHODS: In this single-arm, phase 1/2 trial in five US academic cancer centres, patients (aged >/=18 years) with histologically confirmed metastatic clear-cell renal cell carcinoma, Eastern Cooperative Oncology Group performance status of 1 or less, and adequate organ functions were included. The phase 1 portion included patients with up to one previous therapy with high-dose IL-2 or an anti-PD-1 compound alone or in combination with a CTLA-4 inhibitor. The phase 2 portion enrolled only patients with treatment-naive metastatic clear-cell renal cell carcinoma. The primary endpoint of phase 1 was to find the recommended phase 2 dose (RP2D). We tested a novel regimen using four dose levels: oral pazopanib (600 or 800 mg daily on days 1-28, followed by a 7 day break) alternating with intravenous bevacizumab (5, 7.5, or 10 mg/kg, one on day 36 and one on day 50) on a 10-week cycle in patients with metastatic clear-cell renal cell carcinoma naive to TKI treatment. The primary endpoint of phase 2 was median progression-free survival, assessed by a masked central statistician review. Dose selection (in patients who had completed 15 weeks) and activity (in patients who had completed one full 10-week cycle of the novel regimen) were analysed in the per-protocol population. Safety analyses included all patients who received any treatment. This trial was registered with ClinicalTrials.gov (NCT01684397) and is ongoing but closed to new participants. FINDINGS: Between Nov 21, 2012, and June 11, 2018, phase 1 enrolled 25 patients, with 18 patients evaluable for dose-limiting toxicity during the extended 15-week safety assessment period. The RP2D was established as pazopanib 800 mg daily (days 1-28) and bevacizumab 10 mg/kg on days 36 and 50 of a 10-week cycle. Between Jan 23, 2019, and Oct 19, 2023, phase 2 enrolled 53 patients, of whom 51 (including six from phase 1) were evaluable for activity and 59 were evaluable for safety. 36 (71%) patients were male and 15 (29%) were female, and 48 (94%) patients were White. 33 (65%) patients in phase 2 had favourable risk per the International Metastatic Renal Cell Carcinoma Database Consortium categories. After a median follow-up of 63.7 months (IQR 16.2-73.5), median progression-free survival was 24.8 months (95% CI 17.2-34.3). The most common grade 3-4 treatment-related adverse events were hypertension (20 [34%] of 59) and elevated aspartate aminotransferase (seven [12%]) and alanine aminotransferase (ten [17%]). Five (8%) patients discontinued the study for drug-related serious adverse events. No treatment-related deaths occurred. INTERPRETATION: The phase 1 portion identified the safe RP2D. The phase 2 portion showed that this novel regimen has promising antitumour activity in patients with metastatic clear-cell renal cell carcinoma in the predominantly favourable risk group who are naive to treatment. Phase 3 randomised controlled trial data are needed to confirm these findings. FUNDING: The Roswell Park Comprehensive Cancer Center (RPCCC), US National Cancer Institute (NCI), Novartis. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY-NC-ND 4.0 license. Published by Elsevier Ltd.. All rights reserved. FAU - George, Saby AU - George S AD - Department of Medicine, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA; Department of Medicine, Jacobs' School of Medicine at SUNY Buffalo, Buffalo, NY, USA. Electronic address: saby.george@roswellpark.org. FAU - Vaishampayan, Ulka AU - Vaishampayan U AD - Department of Medicine, University of Michigan, Ann Arbor, MI, USA. FAU - Hutson, Alan AU - Hutson A AD - Department of Biostatistics, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA. FAU - Groman, Adrienne AU - Groman A AD - Department of Biostatistics, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA. FAU - Pili, Roberto AU - Pili R AD - Department of Medicine, Jacobs' School of Medicine at SUNY Buffalo, Buffalo, NY, USA. FAU - Green, Kelly AU - Green K AD - Department of Medicine, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA. FAU - Herbst, Laurie AU - Herbst L AD - Department of Medicine, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA. FAU - Kallicharan-Smith, Rushka AU - Kallicharan-Smith R AD - Department of Medicine, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA. FAU - DiRaddo, Ann-Marie AU - DiRaddo AM AD - Department of Medicine, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA. FAU - Farrell, Elongia AU - Farrell E AD - Department of Medicine, Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA. FAU - Gopalakrishnan, Dharmesh AU - Gopalakrishnan D AD - Department of Medicine, The Cleveland Clinic, Abu Dhabi, United Arab Emirates. FAU - Heath, Elisabeth AU - Heath E AD - Department of Oncology, The Mayo Clinic, Rochester, MN, USA. FAU - Wulff, Elizabeth AU - Wulff E AD - Division of Medical Oncology, Kansas University Medical Center, Westwood, KS, USA. FAU - Kilari, Deepak AU - Kilari D AD - Division of Medical Oncology, Medical College of Wisconsin, Milwaukee, WI, USA. FAU - Appleman, Leonard AU - Appleman L AD - Department of Medicine, University of Pittsburgh, PA, USA. FAU - Parikh, Rahul AU - Parikh R AD - Division of Medical Oncology, Kansas University Medical Center, Westwood, KS, USA. LA - eng SI - ClinicalTrials.gov/NCT01684397 PT - Journal Article DEP - 20261027 PL - England TA - Lancet Oncol JT - The Lancet. Oncology JID - 100957246 SB - IM COIS- Declaration of interests SG reports consulting fees from Astellas, AstraZeneca, Aveo, BMS, Bayer, Eisai, EMD, Exelixis, Glaxo Smith Kline, Johnson and Johnson, Merck, Novartis, Sanofi, and Seattle Genetics; institutional research support from Agensys, Aravive, Aveo, Bayer, BMS, Eisai, Exelixis, Gilead, Merck, Novartis, Pfizer, Seattle Genetics, and Surface Oncology; and expert testimony with BMS, all outside the submitted work. UV reports grants or contracts with BMS and Merck; consulting fees from AstraZeneca, Bayer, BMS, Exelixis, Merck, Novartis, and Pfizer; and a leadership role on the Michigan Society of Hematology and Oncology, all outside the submitted work. EH reports consulting fees from Astellas, AstraZeneca, Bayer, IDEOlogy Health, Novartis, and Sanofi; institutional grant support from American Cancer Society, Arcus Biosciences, Arvinas, Astellas, Astra Zeneca, Bayer, BioXCel Therapeutics, Bristol Myers Squibb, Calibr, Calithera Biosciences, Caris Life Sciences, Clarity Pharmaceuticals, Corcept Therapeutics, Corvus Pharmaceuticals, Daiichi Sankya, the US Department of Defense, Eisai, Exelixis, Five Prime Therapeutics, Fortis, Gilead Sciences, Genentech, Prostate Cancer Foundation, Mayo Clinic Cancer Center, Glaxo Smith Kline, Harpoon Therapeutics, Janssen Research & Development, Janssen Scientific Affairs, ImmunityBio, Infinity Pharmaceuticals, ITeos Therapeutics, Janux Therapeutics, K36 Therapeutics, MacroGenics, Mayo Clinic, Mayo Clinic Development-Beerman Family Foundation, Merck, Merck Sharp & Dohme, Mirati Therapeutics, Modra Pharmaceuticals, National Cancer Institute, Novartis Pharmaceuticals, Oncolys Biopharma, Peloton Therapeutics, Pfizer, Pharmacyclics, POINT Biopharma, Prostate Cancer Foundation, Regeneron Pharmaceuticals, Roche, Seattle Genetics, US Army Medical Research Acquisition Activity, and Xencor; payment or honoraria for presentations by Curio Science, IDEOlogy Health, Janssen Biotech, Petauri Kinect, Prostate Cancer Research Institute, Research to Practice, Sanofi, Seattle Genetics, Sumitomo; paid travel by IDEOlogy Health; participation on an advisory board for Caris Centers of Excellence, EMD Serono, Genzyme, Gilead Sciences, Novartis, Pfizer; and a leadership role on National Comprehensive Cancer Network Board of Directors, all outside the submitted work. EW reports consulting fees from Astra Zeneca; paid travel by Exelixis, Bayer, and IDEOlogy Health; participation in an advisory board for Astellas, Aveo Oncology, Bayer, BMS, Exelixis, Janssen, and Pfizer; and payment or honoraria for presentations by Curio Science, IDEOlogy Health, MJH Life Sciences, Quintescience, and Targeted Oncology, all outside the submitted work. DK reports consulting fees from Astellas, Bayer, Eisai, Exelixis, Foundation Medicine, Merck, and Pfizer; and honoraria for lectures or presentations by Astellas, Aveo Oncology, Eisai, and MJH Life Sciences, all outside the submitted work. All other authors declare no competing interests. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:52 PHST- 2026/01/08 00:00 [received] PHST- 2026/08/03 00:00 [revised] PHST- 2026/08/05 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:52 [entrez] AID - S1470-2045(26)00394-3 [pii] AID - 10.1016/S1470-2045(26)00394-3 [doi] PST - aheadofprint SO - Lancet Oncol. 2026 Oct 27:S1470-2045(26)00394-3. doi: 10.1016/S1470-2045(26)00394-3. PMID- 42945976 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 2331-2165 (Electronic) IS - 2331-2165 (Linking) VI - 30 DP - 2026 Jan-Dec TI - Characterizing Suprathreshold Auditory Function: A Comparison of 22 Direct and Derived Auditory Measures. PG - 23312165261496756 LID - 10.1177/23312165261496756 [doi] AB - A substantial proportion of the population has clinically normal hearing thresholds yet experiences difficulty understanding speech in background noise. Existing hearing models explain these difficulties using a limited number of physiological and signal-processing mechanisms, implying that relationships among psychoacoustic measures may reflect shared underlying processes. However, these relationships have rarely been examined comprehensively within the same participants. Here we quantified associations among psychoacoustic measures using linear regression and principal component analysis. Speech-in-noise, tone-in-noise in different noise types, and loudness growth functions were obtained and analyzed in monaural and binaural listening conditions in 52 volunteers (aged 20-80 years) with age-typical or mild hearing loss (PTA4 < 32.5 dB HL). Linear regression analyses identified direct psychoacoustic measures - particularly interaurally antiphasic tone-in-noise (N0Spi) thresholds - as robust indicators of suprathreshold hearing function. Although N0Spi showed only a moderate association with the corresponding diotic thresholds (N0S0), it was more strongly associated with poorer-ear speech-in-noise thresholds (r = 0.60) than the corresponding 500-Hz audiometric threshold (r = 0.35), suggesting that this relationship was not driven by audibility alone. Exploratory principal component analysis further showed that N0Spi thresholds contributed to multiple auditory dimensions associated with speech-in-noise variability. In contrast, derived measures such as binaural masking level difference or auditory filter bandwidth showed weaker and less consistent associations. Overall, the results indicate that selected direct psychoacoustic measures may provide a more robust characterization of suprathreshold auditory function than derived measures. FAU - Isserstedt, Niklas AU - Isserstedt N AUID- ORCID: 0009-0007-3406-962X AD - Department of Medical Physics and Acoustics, Carl von Ossietzky Universitat Oldenburg, Oldenburg, Germany. RINGGOLD: 11233 AD - Cluster of Excellence "Hearing4All", University of Oldenburg, Oldenburg, Germany. FAU - Schell-Majoor, Lena AU - Schell-Majoor L AUID- ORCID: 0009-0000-2282-8608 AD - Department of Medical Physics and Acoustics, Carl von Ossietzky Universitat Oldenburg, Oldenburg, Germany. RINGGOLD: 11233 AD - Cluster of Excellence "Hearing4All", University of Oldenburg, Oldenburg, Germany. FAU - Jacoby, Nori AU - Jacoby N AUID- ORCID: 0000-0003-2868-0100 AD - Computational Auditory Perception Research Group, Max Planck Institute for Empirical Aesthetics, Frankfurt am Main, Germany. RINGGOLD: 376672 AD - Department of Psychology, Cornell University, Ithaca, NY, USA. FAU - Kollmeier, Birger AU - Kollmeier B AD - Department of Medical Physics and Acoustics, Carl von Ossietzky Universitat Oldenburg, Oldenburg, Germany. RINGGOLD: 11233 AD - Cluster of Excellence "Hearing4All", University of Oldenburg, Oldenburg, Germany. FAU - Dietz, Mathias AU - Dietz M AUID- ORCID: 0000-0002-1830-469X AD - Department of Medical Physics and Acoustics, Carl von Ossietzky Universitat Oldenburg, Oldenburg, Germany. RINGGOLD: 11233 AD - Cluster of Excellence "Hearing4All", University of Oldenburg, Oldenburg, Germany. LA - eng PT - Comparative Study PT - Journal Article DEP - 20261027 PL - United States TA - Trends Hear JT - Trends in hearing JID - 101635698 SB - IM MH - Humans MH - Aged MH - Adult MH - Middle Aged MH - *Auditory Threshold MH - Female MH - Male MH - Aged, 80 and over MH - Psychoacoustics MH - Young Adult MH - Acoustic Stimulation MH - *Speech Perception MH - Noise/adverse effects MH - Audiometry, Pure-Tone MH - Perceptual Masking MH - Linear Models MH - Loudness Perception MH - Principal Component Analysis MH - Auditory Acuity MH - *Hearing MH - *Hearing Loss/physiopathology/diagnosis/psychology MH - Hearing Loss, Hidden OTO - NOTNLM OT - Audiometric thresholds OT - N0Spi and BMLD OT - Power spectrum model OT - Suprathreshold hearing loss COIS- Declaration of Conflicting InterestsThe authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 19:43 PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:43 [entrez] AID - 10.1177/23312165261496756 [doi] PST - ppublish SO - Trends Hear. 2026 Jan-Dec;30:23312165261496756. doi: 10.1177/23312165261496756. Epub 2026 Oct 27. PMID- 42945973 OWN - NLM STAT- Publisher LR - 20261027 IS - 1438-8812 (Electronic) IS - 0013-726X (Linking) DP - 2026 Oct 27 TI - Combined enteroscopy and choledochoscopy for recanalization of complete bilioenteric anastomotic occlusion after pancreaticoduodenectomy: a novel minimally invasive approach. LID - 10.1055/a-2976-5877 [doi] FAU - Huang, Xin AU - Huang X AUID- ORCID: 0000-0002-2265-4254 AD - The Ninth Medical Center of Chinese PLA General Hospital, Department of Gastroenterology, Beijing, China, Beijing. FAU - Zhang, Wenjuan AU - Zhang W AD - The Ninth Medical Center of Chinese PLA General Hospital, Department of Gastroenterology, Beijing, China, Beijing. FAU - Zhong, Changqing AU - Zhong C AD - The Ninth Medical Center of Chinese PLA General Hospital, Department of Gastroenterology, Beijing, China, Beijing. FAU - Lin, Kai AU - Lin K AD - The Ninth Medical Center of Chinese PLA General Hospital, Department of Gastroenterology, Beijing, China, Beijing. FAU - Liang, Bin AU - Liang B AD - The Ninth Medical Center of Chinese PLA General Hospital, Department of Hepatobiliary and Pancreatic Surgery, Beijing, China, Beijing. FAU - Zheng, Shaojie AU - Zheng S AD - The Ninth Medical Center of Chinese PLA General Hospital, Department of Gastroenterology, Beijing, China, Beijing. FAU - Li, Lianyong AU - Li L AUID- ORCID: 0000-0002-0852-7005 AD - The Ninth Medical Center of Chinese PLA General Hospital, Department of Gastroenterology, Beijing, China, Beijing. LA - eng PT - Journal Article DEP - 20261027 PL - Germany TA - Endoscopy JT - Endoscopy JID - 0215166 SB - IM COIS- The authors declare that they have no conflict of interest. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:43 PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:43 [entrez] AID - 10.1055/a-2976-5877 [doi] PST - aheadofprint SO - Endoscopy. 2026 Oct 27. doi: 10.1055/a-2976-5877. PMID- 42945971 OWN - NLM STAT- Publisher LR - 20261027 IS - 1438-9010 (Electronic) IS - 1438-9010 (Linking) DP - 2026 Oct 27 TI - Artificial Intelligence in Radiology: A Position Paper of the Austrian Radiological Society (ORG). LID - 10.1055/a-2965-2624 [doi] AB - ABSTRACT: This position paper was written at the request of the executive board of the Austrian Radiological Society. As a co-opted member of the executive board of the Austrian Radiological Society for digitalization and artificial intelligence (AI), the corresponding author was tasked with assembling an interdisciplinary group of authors. The authors were selected on the basis of their scientific and technical expertise, their position at clinical and university facilities, and their involvement in national and international professional societies and working groups related to AI in radiology. Various radiology subspecialties, the major university health care regions in Austria, and health facilities on various care levels are represented. Experts from the fields of technology, law, and ethics were also included. The referenced literature is based on targeted research without a systematic meta-analysis. The individual chapters were written by topic-specific subgroups and the chapters were then combined by the first author to create this manuscript. The Delphi method was not used. The expert consensus was reviewed by the executive board of the Austrian Radiological Society and approved as the official position of the society after revision. KEY POINTS: . Safe implementation of commercial AI requires standardized local validation, clearly defined responsibilities, continuous performance monitoring, version control, and established procedures for error reporting, revalidation, and deactivation.. . AI performance cannot be established by regulatory approval or technical benchmarks alone but must be demonstrated under local clinical conditions.. . Human responsibility remains essential despite increasing automation.. . AI is shifting the role of radiology from primarily image interpretation toward an increasingly data-integrative, prognostic, and interdisciplinary discipline.. . The value of AI should be assessed by patient-relevant outcomes and effects on healthcare delivery rather than by technical performance or workforce reduction alone.. CITATION FORMAT: . Kau T, Furtner-Srajer J, Adelsmayr G et al. Artificial Intelligence in Radiology: A Position Paper of the Austrian Radiological Society (ORG). Rofo 2026; DOI 10.1055/a-2965-2624. CI - The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/). FAU - Kau, Thomas AU - Kau T AD - Villach Regional Hospital, Institute of Diagnostic and Interventional Radiology, Austria, Villach. AD - Medical University of Graz, Department of Radiology, Austria, Graz. FAU - Furtner-Srajer, Julia AU - Furtner-Srajer J AD - Danube Private University, Research Center for Medical Image Analysis and Artificial Intelligence (MIAAI), Austria, Krems an der Donau. FAU - Adelsmayr, Gabriel AU - Adelsmayr G AUID- ORCID: 0000-0002-7849-662X AD - Medical University of Graz, Radiology, Austria, Graz. FAU - Widmann, Gerlig AU - Widmann G AD - Medical University of Innsbruck, Department of Radiology, Austria, Innsbruck. FAU - Neumann, Christian AU - Neumann C AD - Hospital of St. John of God, Department of Radiology and Nuclear Medicine, Austria, Vienna. FAU - Perkonigg, Matthias AU - Perkonigg M AD - Medical University of Innsbruck, Institute of Neuropathology and Neuromolecular Pathology, Austria, Innsbruck. AD - Medical University of Vienna, Department of Biomedical Imaging and Image-guided Therapy, Austria, Vienna. FAU - Woitek, Ramona AU - Woitek R AD - Danube Private University, Research Center for Medical Image Analysis and Artificial Intelligence, Department of Medicine, Austria, Krems an der Donau. FAU - Lang, Kristina AU - Lang K AD - Lund University Faculty of Medicine, Division of Diagnostic Radiology, Department of Translational Medicine, Sweden, Lund. AD - Skane University Hospital Lund, Unilabs Mammography Unit, Sweden, Lund. FAU - Rubeis, Giovanni AU - Rubeis G AD - University of Graz, Division of Health Care Ethics, Austria, Graz. FAU - Pedevilla, Markus AU - Pedevilla M AD - KAGes, Directorate for Digitalization, IT, Organizational Development and Innovation, Austria, Graz. FAU - Gruber, Michael AU - Gruber M AD - Radiology Group Practice Baden, Radiological Group Practice, Austria, Baden. FAU - Prosch, Helmut AU - Prosch H AUID- ORCID: 0000-0002-6119-6364 AD - Medical University of Vienna, Department of General and Pediatric Radiology, Austria, Vienna. FAU - Schuster, Antonius AU - Schuster A AD - Regional Hospital of Bregenz, Department of Radiology, Austria, Bregenz. FAU - Forgo, Nikolaus AU - Forgo N AD - University of Vienna Faculty of Law, Department of Innovation and Digitalisation in Law, Austria, Vienna. FAU - Cadamuro, Janne AU - Cadamuro J AD - Paracelsus Medical University Salzburg - private foundation, Department of Laboratory Medicine, Austria, Salzburg. FAU - Prainsack, Barbara AU - Prainsack B AD - University of Vienna Faculty of Social Sciences, Department of Political Science, Austria, Vienna. FAU - Fuchsjager, Michael H AU - Fuchsjager MH AD - Medical University of Graz, Department of Radiology, Austria, Graz. FAU - Langs, Georg AU - Langs G AD - Medical University of Vienna, Department of Biomedical Imaging and Image-guided Therapy, Computational Imaging Research Lab, Austria, Vienna. FAU - Uggowitzer, Martin Michael AU - Uggowitzer MM AD - Hochsteiermark Regional Hospital Leoben Location, Department of Radiology and Nucelar Medicine, Austria, Leoben. AD - Medical University of Graz, Division of General Radiology, Department of Radiology, Austria, Graz. LA - eng LA - ger PT - Journal Article TT - Kunstliche Intelligenz in der Radiologie: Positionspapier der Osterreichischen Rontgengesellschaft (ORG). DEP - 20261027 PL - Germany TA - Rofo JT - RoFo : Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin JID - 7507497 SB - IM COIS- Georg Langs ist Mitbegrunder und Gesellschafter von CONTEXTFLOW, einem Unternehmen fur medizinische KI. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:42 PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:42 [entrez] AID - 10.1055/a-2965-2624 [doi] PST - aheadofprint SO - Rofo. 2026 Oct 27. doi: 10.1055/a-2965-2624. PMID- 42945970 OWN - NLM STAT- Publisher LR - 20261029 IS - 1879-0445 (Electronic) IS - 0960-9822 (Linking) DP - 2026 Oct 27 TI - Primary cilia mediate specific neuron-glia attachments in C. elegans through the secreted protein BUG-1. LID - S0960-9822(26)01337-0 [pii] LID - 10.1016/j.cub.2026.10.003 [doi] AB - Primary cilia are signaling hubs that influence nearly every aspect of cell physiology. Neuronal primary cilia were recently found to form extensive attachments to glia in the mammalian brain, but it is not known how these attachments form or what functions they serve. Defects in cilia cause profound neurological disorders, highlighting the importance of understanding the specialized functions of neuronal cilia. Here, we use a genetic screen in Caenorhabditis elegans to determine how a stereotyped cilia-glia attachment forms and how the attachment affects ciliary calcium signaling. We find that two sensory neurons (URX and BAG) use their cilia to attach to a specific glial partner (ILsoL/R). This attachment requires BUG-1, a large secreted protein that localizes precisely to the cilia-glia attachment site. In the absence of BUG-1, neuronal cilia are present but always fail to attach to the glial cell. As a result, the URX cilia do not undergo activity-dependent remodeling seen in wild-type neurons and the BAG cilia exhibit reduced sensory stimulus-evoked calcium entry and defects in calcium clearance. Our work shows that cilia can select specific glial partners for attachment, identifies a novel protein that mediates these attachments, and demonstrates the functional consequences of loss of cilia-glia attachment on neuronal signaling. We propose that neuronal primary cilia act not only as antennae to receive long-range signals but also as points of close-range cell-cell contact, through which glia could modulate neuronal signaling. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Wexler, Leland R AU - Wexler LR AD - Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA 02115, USA. FAU - Griffin, Brian AU - Griffin B AD - Department of Cell Biology and Neuroscience Institute, New York University School of Medicine, New York, NY 10016, USA. FAU - Dutta, Priya AU - Dutta P AD - Department of Biology, Brandeis University, Waltham, MA 02454, USA. FAU - Sengupta, Piali AU - Sengupta P AD - Department of Biology, Brandeis University, Waltham, MA 02454, USA. FAU - Kolotuev, Irina AU - Kolotuev I AD - Department of Biomedical Sciences, University of Lausanne, 1015 Lausanne, Switzerland. FAU - Ringstad, Niels AU - Ringstad N AD - Department of Cell Biology and Neuroscience Institute, New York University School of Medicine, New York, NY 10016, USA. Electronic address: niels.ringstad@nyulangone.org. FAU - Heiman, Maxwell G AU - Heiman MG AD - Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA 02115, USA. Electronic address: maxwell.heiman@childrens.harvard.edu. LA - eng GR - R01 NS112343/NS/NINDS NIH HHS/United States GR - R01 NS124879/NS/NINDS NIH HHS/United States PT - Journal Article DEP - 20261027 PL - England TA - Curr Biol JT - Current biology : CB JID - 9107782 SB - IM OTO - NOTNLM OT - C. elegans OT - cell attachment OT - cilia OT - dendrites OT - glia OT - neurodevelopment OT - sensory neurons COIS- Declaration of interests The authors declare no competing interests. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:42 PHST- 2026/05/22 00:00 [received] PHST- 2026/08/25 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:42 [entrez] AID - S0960-9822(26)01337-0 [pii] AID - 10.1016/j.cub.2026.10.003 [doi] PST - aheadofprint SO - Curr Biol. 2026 Oct 27:S0960-9822(26)01337-0. doi: 10.1016/j.cub.2026.10.003. PMID- 42945961 OWN - NLM STAT- Publisher LR - 20261027 IS - 1878-3686 (Electronic) IS - 1535-6108 (Linking) DP - 2026 Oct 27 TI - CPS + EG and Neo-Bioscore predict individual outcomes but not trial results after neoadjuvant therapy for early breast cancer. LID - S1535-6108(26)00437-X [pii] LID - 10.1016/j.ccell.2026.10.002 [doi] AB - CPS + EG and Neo-Bioscore are prognostic scores estimating the risk of recurrence after neoadjuvant chemotherapy for early breast cancer, but can they predict the long-term outcomes in a clinical trial? In this study, we assessed their surrogacy values at patient level and trial level for long-term outcomes, using data from 8,359 patients across nine neoadjuvant randomized trials conducted by the German Breast Group (GBG) and AGO-B study group. We found that at the patient level, both scores were significantly associated with outcomes, but at the trial level, the associations with distant disease-free survival (DDFS), invasive disease-free survival (iDFS), and overall survival (OS) were weak for both scores (R(2) range = 0.03 to 0.24, with wide confidence intervals). Thus, our findings suggest that CPS + EG and Neo-Bioscore are not reliable surrogate endpoints for predicting long-term treatment benefit in neoadjuvant trials but remain valuable for estimating individual patients' residual risk after neoadjuvant treatments and guiding subsequent treatment decisions for early breast cancer patients. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Conforti, Fabio AU - Conforti F AD - Medical Oncology Unit, Humanitas Gavazzeni, Bergamo, Italy; Humanitas University, Rozzano, Italy. Electronic address: fabio.conforti1984@gmail.com. FAU - Nekljudova, Valentina AU - Nekljudova V AD - German Breast Group, Neu-Isenburg, Germany. FAU - Holtschmidt, Johannes AU - Holtschmidt J AD - German Breast Group, Neu-Isenburg, Germany. FAU - Sala, Isabella AU - Sala I AD - Department of Statistics and Quantitative Methods, University of Milano-Bicocca, Milan, Italy; School of Medicine and Surgery, University of Milano-Bicocca, Milan, Italy. FAU - Pagan, Eleonora AU - Pagan E AD - Department of Statistics and Quantitative Methods, University of Milano-Bicocca, Milan, Italy. FAU - Solbach, Christine AU - Solbach C AD - University Hospital Frankfurt, Frankfurt, Germany. FAU - Untch, Michael AU - Untch M AD - Helios Kliniken, Berlin-Buch, Germany. FAU - Denkert, Carsten AU - Denkert C AD - Institute of Pathology, Philipps-University Marburg, University Hospital UKGM Marburg, Marburg, Germany. FAU - Bagnardi, Vincenzo AU - Bagnardi V AD - Department of Statistics and Quantitative Methods, University of Milano-Bicocca, Milan, Italy. FAU - Bendoni, Marzia AU - Bendoni M AD - Humanitas University, Rozzano, Italy. FAU - Fasching, Peter A AU - Fasching PA AD - Universitatsklinikum Erlangen, Erlangen, Germany. FAU - Schneeweiss, Andreas AU - Schneeweiss A AD - National Center for Tumor Diseases, University Hospital and German Cancer Research Center, Heidelberg, Germany. FAU - Luck, Hans-Joachim AU - Luck HJ AD - Gynakologisch-onkologische Praxis Neumarkt, Neumarkt, Germany. FAU - Valenza, Carmine AU - Valenza C AD - European Institute of Oncology, IRCCS, Milan, Italy. FAU - Viale, Giuseppe AU - Viale G AD - European Institute of Oncology, IRCCS, Milan, Italy. FAU - De Pas, Tommaso AU - De Pas T AD - Medical Oncology Unit, Humanitas Gavazzeni, Bergamo, Italy. FAU - van Mackelenbergh, Marion AU - van Mackelenbergh M AD - Universitatsklinikum Schleswig-Holstein, Klinik fur Gynakologie und Geburtshilfe, Schleswig-Holstein, Germany. FAU - Huober, Jens AU - Huober J AD - Kantonsspital St.Gallen, Brustzentrum, St. Gallen, Switzerland. FAU - Muller, Volkmar AU - Muller V AD - Universitatsklinikum Hamburg-Eppendorf, Hamburg-Nord, Germany. FAU - Link, Theresa AU - Link T AD - Department of Gynecology and Obstetrics, Technische Universitat Dresden, Dresden, Germany. FAU - Karn, Thomas AU - Karn T AD - Klinik fur Frauenheilkunde und Geburtshilfe, Universitatsklinikum Frankfurt, Frankfurt, Germany. FAU - Reinisch, Mattea AU - Reinisch M AD - Breast Unit, Evang. Kliniken Essen-Mitte, Essen-Stadtbezirk I, Germany. FAU - Marme, Frederik AU - Marme F AD - Medizinische Fakultat Mannheim, Universitat Heidelberg, Universitatsfrauenklinik Mannheim, Heidelberg, Germany. FAU - Bjelic-Radisic, Vesna AU - Bjelic-Radisic V AD - Breast Unit, University Hospital Helios, University Witten Herdecke, Wuppertal, Germany. FAU - Schem, Christian AU - Schem C AD - Mammazentrum Hamburg, Eimsbuttel, Germany. FAU - Hartkopf, Andreas AU - Hartkopf A AD - AGO Study Group and University Hospital Tubingen, Tubingen, Germany. FAU - Stickeler, Elmar AU - Stickeler E AD - Klinik fur Gynakologie und Geburtsmedizin, Uniklinik Aachen, Aachen, Germany. FAU - Hanusch, Claus AU - Hanusch C AD - Rotkreuzklinikum Munich, Munich, Germany. FAU - Blohmer, Jens-Uwe AU - Blohmer JU AD - Gynakologie Mit Brustzentrum, Charite Universitatsmedizin Berlin, Berlin, Germany. FAU - Fehm, Tanja AU - Fehm T AD - Universitatsklinik Dusseldorf, Dusseldorf, Germany. FAU - Rhiem, Kerstin AU - Rhiem K AD - Center for Familial Breast and Ovarian Cancer, University of Cologne, Cologne, Germany. FAU - Gelber, Richard D AU - Gelber RD AD - Department of Data Science, Dana-Farber Cancer Institute, Harvard Medical School, Harvard T.H. Chan School of Public Health, and Frontier Science & Technology Research Foundation, Boston, MA, USA. FAU - Loibl, Sibylle AU - Loibl S AD - German Breast Group, Neu-Isenburg, Germany. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Cancer Cell JT - Cancer cell JID - 101130617 SB - IM OTO - NOTNLM OT - CPS + EG systems OT - Neo-Bioscore systems OT - early breast cancer OT - neoadjuvant randomized control trials OT - patient-level surrogacy OT - surrogate endpoints OT - trial-level surrogacy COIS- Declaration of interests V.N. is a GBG Forschungs GmbH employee. GBG Forschungs GmbH received funding for research grants from Abbvie, Amgen, AstraZeneca, BMS, Daiichi-Sankyo, Gilead, Molecular Health, Novartis, Pfizer, and Roche (paid to the institution) and other (non-financial/medical writing) from Daiichi-Sankyo, Gilead, Novartis, Pfizer, Roche, and Seagen (paid to the institution). GBG Forschungs GmbH has licensing fees from VMscope GmbH. In addition, GBG Forschungs GmbH has the following pending patents: EP21152186.9, EP19808852.8, and EP14153692.0. T.L. has received honoraria from Amgen, Roche, MSD, Novartis, Pfizer, Lilly, GSK, Gilead, AstraZeneca, Daiichi Sankyo, Seagen, Stemline and non-financial support from Pfizer, AstraZeneca, Gilead, Daiichi Sankyo, and Stemline. T.L. participates in advisory boards from MSD, Roche, Pfizer, Lilly, Myriad, Esai, GSK, Gilead, Daiichi Sankyo, and Roche. R.D.G. reports that his institution receives support for his salary from AstraZeneca, Merck, and Roche. M.U. reports honoraria from AstraZeneca, Art tempi, Amgen, Daiji Sankyo, Lilly, Roche, Pfizer, MSD Oncology, Pierre Fabre, Sanofi-Aventis, Myriad, Seagen, Gilead, Novartis, and Stemline and also serves a consulting or advisory role for Amgen, Lilly, Roche, Pfizer, Lilly, Pierre Fabre, Novartis, MSD Oncology, Roche, Agendia, Seagen, Gilead, Lily, Stemline, Genzyme, and Onkowissen.de. All honoraria and fees to the employer/institution. C. Schem declares honoraria for speakers bureau and advice from Roche, Novartis, MSD, AstraZeneca, Novartis, and Lilly and travel grants from Pfizer, Lilly, Roche, Novartis, and AstraZeneca. C.D. reports grants from European Commission H2020, German Cancer Aid Translational Oncology, German Breast Group, and BMBF to the institution during the conduct of the study; personal fees from Novartis, Roche, MSD Oncology, Daiichi Sankyo, AstraZeneca, and Molecular Health; grants from Myriad; personal fees from Merck; and other from Sividon diagnostics outside the submitted work. In addition, C.D. has a patent VMScope digital pathology software with royalties paid, a patent WO2020109570A1 - cancer immunotherapy pending, and a patent WO2015114146A1, and WO2010076322A1-therapy response issued. S.L. is a GBG Forschungs GmbH employee (CEO); receives grants from AbbVie, AstraZeneca, Celgene, Daiichi-Sankyo, Immunomedics/Gilead, Molecular Health, Novartis, Pfizer, and Roche; receives honoraria for Advisory board from Abbvie, Amgen, AstraZeneca, BMS, Celgene, DSI, EirGenix, Gilead, GSK, Lilly, Merck, Novartis, Olema, Pfizer, Pierre Fabre, Relay Therapeutics, Roche, Sanofi, and Seagen; and receives honoraria as invented speaker from AstraZeneca, DSI, Gilead, Novartis, Pfizer, Roche, Seage, and Medscape. S.L. also reports non-financial interests in an advisory role in AGO Kommission Mamma and a principal investigator role (Aphinity); is a member of AGO, ASCO, DKG, ESMO; and has other non-financial interests in AstraZeneca, Daiichi-Sankyo, Immunomedics/Gilead, Novartis, Pfizer, Roche, and Seagen. GBG Forschungs GmbH has the following royalties/patents: EP14153692.0, EP21152186.9, EP15702464.7, EP19808852.8, and VM Scope GmbH. J. Holtschmidt reports personal fees and non-financial support from Daiichi Sankyo; non-financial support from Hologic; and personal fees from MSD Oncology, Novartis, Palleos Health Care, Pfizer, Roche Pharma and Seagen outside the submitted work. J. Holtschmidt is also a GBG Forschungs GmbH employee. GBG Forschungs GmbH received funding for research grants from Abbvie, Amgen, AstraZeneca, BMS, Daiichi-Sankyo, Gilead, Molecular Health, Novartis, Pfizer, and Roche (paid to the institution) and other (non-financial/medical writing) from Daiichi-Sankyo, Gilead, Novartis, Pfizer, Roche and Seagen (paid to the institution). GBG Forschungs GmbH has licensing fees from VMscope GmbH. In addition, GBG Forschungs GmbH has a patent EP21152186.9 pending, a patent EP19808852.8 pending, and a patent EP14153692.0 pending. F.C. affirms that this manuscript is an honest, accurate, and transparent account of the study being reported; that no important aspects of the study have been omitted; and that any discrepancies from the study as planned have been explained. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:42 PHST- 2026/06/05 00:00 [received] PHST- 2026/08/29 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:42 [entrez] AID - S1535-6108(26)00437-X [pii] AID - 10.1016/j.ccell.2026.10.002 [doi] PST - aheadofprint SO - Cancer Cell. 2026 Oct 27:S1535-6108(26)00437-X. doi: 10.1016/j.ccell.2026.10.002. PMID- 42945959 OWN - NLM STAT- Publisher LR - 20261027 IS - 1878-3686 (Electronic) IS - 1535-6108 (Linking) DP - 2026 Oct 27 TI - Macrophage lipid signaling drives glioblastoma invasion and represents a therapeutic vulnerability. LID - S1535-6108(26)00472-1 [pii] LID - 10.1016/j.ccell.2026.10.006 [doi] AB - Glioblastoma (GBM) is characterized by diffuse infiltration into the surrounding brain, limiting complete surgical resection, the strongest determinant of patient survival. The mechanisms driving this invasive growth remain incompletely understood. Here, we identify a lipid-mediated paracrine signaling axis through which tumor-associated microglia/macrophages promote glioma invasion. Integrating single-cell transcriptomics, spatial lipidomics, and functional perturbation across mouse models and human GBM samples, we show that invading tumor cells engage and reprogram myeloid cells via colony-stimulating factor-1 receptor (CSF1R)-PI3K signaling. This induces lipid metabolic reprogramming and secretion of bioactive lipids, including lysophosphatidylcholines (LPCs) and lysophosphatidic acids (LPAs), which promote GBM invasion through distinct downstream pathways. Disruption of this myeloid-GBM axis reduces lipid secretion and suppresses invasion. Targeting downstream LPA-LPA receptor (LPAR) or YAP/TAZ signaling further constrains invasion in a context-dependent manner. Together, these findings define a lipid-driven circuit linking the tumor microenvironment to glioma invasion and identify strategies to reduce infiltration and improve surgical resection. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Pallares-Moratalla, Carla AU - Pallares-Moratalla C AD - Laboratory of Tumor Microenvironment and Therapeutic Resistance, VIB-KU Leuven Center for Cancer Biology, 3000 Leuven, Belgium; Department of Oncology, KU Leuven, 3000 Leuven, Belgium. FAU - Guyot, Melanie AU - Guyot M AD - Laboratory of Tumor Microenvironment and Therapeutic Resistance, VIB-KU Leuven Center for Cancer Biology, 3000 Leuven, Belgium; Department of Oncology, KU Leuven, 3000 Leuven, Belgium. FAU - Gopalan, Krish Skandha AU - Gopalan KS AD - Laboratory of Tumor Microenvironment and Therapeutic Resistance, VIB-KU Leuven Center for Cancer Biology, 3000 Leuven, Belgium; Department of Oncology, KU Leuven, 3000 Leuven, Belgium. FAU - Robl, Bernhard AU - Robl B AD - Laboratory of Tumor Microenvironment and Therapeutic Resistance, VIB-KU Leuven Center for Cancer Biology, 3000 Leuven, Belgium; Department of Pediatrics and Adolescent Medicine, Comprehensive Cancer Center and Comprehensive Center for Pediatrics, Medical University of Vienna, 1090 Vienna, Vienna, Austria. FAU - Idkowiak, Jakub AU - Idkowiak J AD - Department of Oncology, KU Leuven, 3000 Leuven, Belgium; Laboratory of Lipid Metabolism and Cancer, KU Leuven, 3000 Leuven, Belgium; KU Leuven Institute for Single Cell Omics (LISCO), 3000 Leuven, Belgium. FAU - Dehairs, Jonas AU - Dehairs J AD - Department of Oncology, KU Leuven, 3000 Leuven, Belgium; Laboratory of Lipid Metabolism and Cancer, KU Leuven, 3000 Leuven, Belgium; KU Leuven Institute for Single Cell Omics (LISCO), 3000 Leuven, Belgium. FAU - Ravoet, Nina AU - Ravoet N AD - Department of Oncology, KU Leuven, 3000 Leuven, Belgium; Laboratory of Lipid Metabolism and Cancer, KU Leuven, 3000 Leuven, Belgium; KU Leuven Institute for Single Cell Omics (LISCO), 3000 Leuven, Belgium. FAU - Hendriks, Tim F E AU - Hendriks TFE AD - The Maastricht MultiModal Molecular Imaging (M4I) Institute, Division of Imaging Mass Spectrometry (IMS), Maastricht University, 6200 MD Maastricht, the Netherlands. FAU - Nowak, Kinga Anna AU - Nowak KA AD - KU Leuven Institute for Single Cell Omics (LISCO), 3000 Leuven, Belgium; Laboratory for Precision Cancer Medicine, Translational Cell and Tissue Research Unit, Department of Imaging & Pathology, KU Leuven, 3000 Leuven, Belgium. FAU - Lakic, Nikolina Dubroja AU - Lakic ND AD - KU Leuven Institute for Single Cell Omics (LISCO), 3000 Leuven, Belgium; Laboratory for Precision Cancer Medicine, Translational Cell and Tissue Research Unit, Department of Imaging & Pathology, KU Leuven, 3000 Leuven, Belgium. FAU - Weng, Linqian AU - Weng L AD - Laboratory of Tumor Microenvironment and Therapeutic Resistance, VIB-KU Leuven Center for Cancer Biology, 3000 Leuven, Belgium; Department of Oncology, KU Leuven, 3000 Leuven, Belgium. FAU - Konstantakos, Vasileios AU - Konstantakos V AD - Laboratory of Computational Biology, VIB Center for AI & Computational Biology (VIB.AI), Leuven, Belgium; VIB-KU Leuven Center for Neuroscience, 3000 Leuven, Belgium; Department of Human Genetics, KU Leuven, 3000 Leuven, Belgium. FAU - Spotbeen, Xander AU - Spotbeen X AD - Department of Oncology, KU Leuven, 3000 Leuven, Belgium; Laboratory of Lipid Metabolism and Cancer, KU Leuven, 3000 Leuven, Belgium; KU Leuven Institute for Single Cell Omics (LISCO), 3000 Leuven, Belgium. FAU - Serneels, Lutgarde AU - Serneels L AD - VIB-KU Leuven Center for Neuroscience, 3000 Leuven, Belgium; Department of Neurosciences and Leuven Brain Institute, KU Leuven, 3000 Leuven, Belgium. FAU - Fendt, Sarah-Maria AU - Fendt SM AD - Department of Oncology, KU Leuven, 3000 Leuven, Belgium; Laboratory of Cellular Metabolism and Metabolic Regulation, VIB Center for Cancer Biology, 3000 Leuven, Belgium; Leuven Cancer Institute (LKI), 3000 Leuven, Belgium. FAU - Aerts, Stein AU - Aerts S AD - Laboratory of Computational Biology, VIB Center for AI & Computational Biology (VIB.AI), Leuven, Belgium; VIB-KU Leuven Center for Neuroscience, 3000 Leuven, Belgium; Department of Human Genetics, KU Leuven, 3000 Leuven, Belgium. FAU - De Smet, Frederik AU - De Smet F AD - KU Leuven Institute for Single Cell Omics (LISCO), 3000 Leuven, Belgium; Laboratory for Precision Cancer Medicine, Translational Cell and Tissue Research Unit, Department of Imaging & Pathology, KU Leuven, 3000 Leuven, Belgium; Leuven Cancer Institute (LKI), 3000 Leuven, Belgium. FAU - Cuypers, Eva AU - Cuypers E AD - The Maastricht MultiModal Molecular Imaging (M4I) Institute, Division of Imaging Mass Spectrometry (IMS), Maastricht University, 6200 MD Maastricht, the Netherlands. FAU - Swinnen, Johannes V AU - Swinnen JV AD - Department of Oncology, KU Leuven, 3000 Leuven, Belgium; Laboratory of Lipid Metabolism and Cancer, KU Leuven, 3000 Leuven, Belgium; KU Leuven Institute for Single Cell Omics (LISCO), 3000 Leuven, Belgium; Leuven Cancer Institute (LKI), 3000 Leuven, Belgium. FAU - Bergers, Gabriele AU - Bergers G AD - Laboratory of Tumor Microenvironment and Therapeutic Resistance, VIB-KU Leuven Center for Cancer Biology, 3000 Leuven, Belgium; Department of Oncology, KU Leuven, 3000 Leuven, Belgium; Leuven Cancer Institute (LKI), 3000 Leuven, Belgium. Electronic address: gabriele.bergers@kuleuven.be. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Cancer Cell JT - Cancer cell JID - 101130617 SB - IM OTO - NOTNLM OT - CSF1-CSF1R signaling OT - LPC/LPA OT - TAM OT - YAP/TAZ signaling OT - glioblastoma OT - invasion OT - lipid metabolism OT - spatial lipidomics OT - tumor-associated microglia/macrophage COIS- Declaration of interests The authors declare no competing interests. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:42 PHST- 2026/03/26 00:00 [received] PHST- 2026/08/25 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:42 [entrez] AID - S1535-6108(26)00472-1 [pii] AID - 10.1016/j.ccell.2026.10.006 [doi] PST - aheadofprint SO - Cancer Cell. 2026 Oct 27:S1535-6108(26)00472-1. doi: 10.1016/j.ccell.2026.10.006. PMID- 42945956 OWN - NLM STAT- Publisher LR - 20261027 IS - 1097-4172 (Electronic) IS - 0092-8674 (Linking) DP - 2026 Oct 27 TI - Deep sequencing of 3,367 maize genomes uncovers substantial contribution of rare variants to agronomic traits. LID - S0092-8674(26)01209-2 [pii] LID - 10.1016/j.cell.2026.09.063 [doi] AB - Rare variants are highly abundant, yet their genome-wide contributions to agronomic traits remain elusive in plants. Here, we performed deep whole-genome sequencing of 3,367 diverse elite maize inbred lines, uncovering 75.3 million variants, of which 79.2% were rare. Overall, we identified 411 independent association signals for 10 agronomic traits, including 359 attributable to globally rare variants, with rare variants showing effect sizes approximately 2.4 times those of common variants. Frequency-stratified genome-based restricted maximum likelihood (GREML) analyses indicated that rare variants captured a substantial proportion of phenotypic variance for traits under strong adaptive selection, consistent with the allele frequencies of trait-associated variants shifting from common in tropical to rare in temperate maize. Complementing conventional variant-level genome-wide association studies (GWAS), gene-based tests identified an additional 94 candidate gene-trait associations. Our study highlights rare variants as important components of the genetic architecture of complex agronomic traits in maize and provides much-needed resources for future functional studies and breeding. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Huang, Liangliang AU - Huang L AD - State Key Laboratory of Maize Bio-breeding, School of Interdisciplinary Life Sciences, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Maize Biology and Genetic Breeding, Key Laboratory of Genome Editing Research and Application (MARA), National Maize Improvement Center, China Agricultural University, Beijing 100193, P.R. China. FAU - Bian, Chao AU - Bian C AD - State Key Laboratory of Maize Bio-breeding, School of Interdisciplinary Life Sciences, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Maize Biology and Genetic Breeding, Key Laboratory of Genome Editing Research and Application (MARA), National Maize Improvement Center, China Agricultural University, Beijing 100193, P.R. China. FAU - Xin, Beibei AU - Xin B AD - State Key Laboratory of Maize Bio-breeding, School of Interdisciplinary Life Sciences, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Maize Biology and Genetic Breeding, Key Laboratory of Genome Editing Research and Application (MARA), National Maize Improvement Center, China Agricultural University, Beijing 100193, P.R. China. FAU - Zhang, Xuecai AU - Zhang X AD - Ministry of Agriculture and Rural Affairs - CIMMYT Maize and Wheat Joint Laboratory, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100081, P.R. China; International Maize and Wheat Improvement Center (CIMMYT), Texcoco 56237, Mexico. FAU - Lu, Qiong AU - Lu Q AD - State Key Laboratory of Maize Bio-breeding, School of Interdisciplinary Life Sciences, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Maize Biology and Genetic Breeding, Key Laboratory of Genome Editing Research and Application (MARA), National Maize Improvement Center, China Agricultural University, Beijing 100193, P.R. China. FAU - Yue, Yang AU - Yue Y AD - State Key Laboratory of Maize Bio-breeding, School of Interdisciplinary Life Sciences, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Maize Biology and Genetic Breeding, Key Laboratory of Genome Editing Research and Application (MARA), National Maize Improvement Center, China Agricultural University, Beijing 100193, P.R. China. FAU - Li, Yingshuang AU - Li Y AD - State Key Laboratory of Maize Bio-breeding, School of Interdisciplinary Life Sciences, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Maize Biology and Genetic Breeding, Key Laboratory of Genome Editing Research and Application (MARA), National Maize Improvement Center, China Agricultural University, Beijing 100193, P.R. China. FAU - Zeng, Rong AU - Zeng R AD - State Key Laboratory of Plant Environmental Resilience, Frontiers Science Center for Molecular Design Breeding, College of Biological Sciences, China Agricultural University, Beijing 100193, P.R. China. FAU - Liu, Xinxiang AU - Liu X AD - Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Maize Research Institute, Beijing Academy of Agriculture and Forestry Sciences (BAAFS), Beijing 100097, P.R. China. FAU - Fan, Xingming AU - Fan X AD - Institute of Food Crops, Yunnan Academy of Agricultural Sciences, Kunming 650205, P.R. China. FAU - Wang, Tianyu AU - Wang T AD - Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China. FAU - Wang, Yanbo AU - Wang Y AD - Institute of Maize Research, Liaoning Academy of Agricultural Sciences, Shenyang 110161, P.R. China. FAU - Cao, Jingsheng AU - Cao J AD - Maize Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R. China. FAU - Zhang, Jianguo AU - Zhang J AD - Maize Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, P.R. China. FAU - Wu, Jianyu AU - Wu J AD - State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Agricultural University, Zhengzhou 450046, P.R. China. FAU - Lin, Zhongwei AU - Lin Z AD - State Key Laboratory of Maize Bio-breeding, School of Interdisciplinary Life Sciences, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Maize Biology and Genetic Breeding, Key Laboratory of Genome Editing Research and Application (MARA), National Maize Improvement Center, China Agricultural University, Beijing 100193, P.R. China. FAU - Zhao, Jiuran AU - Zhao J AD - Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Maize Research Institute, Beijing Academy of Agriculture and Forestry Sciences (BAAFS), Beijing 100097, P.R. China. FAU - Cheng, Weidong AU - Cheng W AD - Maize Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, P.R. China. FAU - Gao, Shibin AU - Gao S AD - Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R. China. FAU - Chen, Yanhui AU - Chen Y AD - State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Agricultural University, Zhengzhou 450046, P.R. China. FAU - Lu, Yuncai AU - Lu Y AD - College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150006, P.R. China. FAU - Yang, Junpin AU - Yang J AD - Crop Research Institute, Sichuan Academy of Agricultural Sciences, Crop Germplasm Center of Sichuan Province, Chengdu 610066, P.R. China. FAU - Liu, Wenguo AU - Liu W AD - Maize Research Institute, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, P.R. China. FAU - Chen, Zehui AU - Chen Z AD - Yazhouwan National Laboratory, Sanya 572024, P.R. China. FAU - Sun, Haiyan AU - Sun H AD - College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, P.R. China. FAU - Lu, Xiaomin AU - Lu X AD - Cereal Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R. China. FAU - Xue, Jiquan AU - Xue J AD - Key Laboratory of Biology and Genetic Improvement of Maize in Arid Area of Northwest Region, College of Agronomy, Northwest A&F University, Yangling 712100, P.R. China. FAU - Yang, Shuhua AU - Yang S AD - State Key Laboratory of Plant Environmental Resilience, Frontiers Science Center for Molecular Design Breeding, College of Biological Sciences, China Agricultural University, Beijing 100193, P.R. China. FAU - Liu, Qiujie AU - Liu Q AD - State Key Laboratory of Maize Bio-breeding, School of Interdisciplinary Life Sciences, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Maize Biology and Genetic Breeding, Key Laboratory of Genome Editing Research and Application (MARA), National Maize Improvement Center, China Agricultural University, Beijing 100193, P.R. China. FAU - Chen, Jian AU - Chen J AD - State Key Laboratory of Maize Bio-breeding, School of Interdisciplinary Life Sciences, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Maize Biology and Genetic Breeding, Key Laboratory of Genome Editing Research and Application (MARA), National Maize Improvement Center, China Agricultural University, Beijing 100193, P.R. China. FAU - Zhao, Hainan AU - Zhao H AD - State Key Laboratory of Maize Bio-breeding, School of Interdisciplinary Life Sciences, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Maize Biology and Genetic Breeding, Key Laboratory of Genome Editing Research and Application (MARA), National Maize Improvement Center, China Agricultural University, Beijing 100193, P.R. China. FAU - Zhao, Haiming AU - Zhao H AD - State Key Laboratory of Maize Bio-breeding, School of Interdisciplinary Life Sciences, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Maize Biology and Genetic Breeding, Key Laboratory of Genome Editing Research and Application (MARA), National Maize Improvement Center, China Agricultural University, Beijing 100193, P.R. China. FAU - Song, Weibin AU - Song W AD - State Key Laboratory of Maize Bio-breeding, School of Interdisciplinary Life Sciences, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Maize Biology and Genetic Breeding, Key Laboratory of Genome Editing Research and Application (MARA), National Maize Improvement Center, China Agricultural University, Beijing 100193, P.R. China; Sanya International Maize Research Center, Sanya Institute of China Agricultural University, Sanya 572025, P.R. China; Center for Crop Functional Genomics and Molecular Breeding, China Agricultural University, Beijing 100193, P.R. China. Electronic address: songwb@cau.edu.cn. FAU - Lai, Jinsheng AU - Lai J AD - State Key Laboratory of Maize Bio-breeding, School of Interdisciplinary Life Sciences, Frontiers Science Center for Molecular Design Breeding (MOE), Key Laboratory of Maize Biology and Genetic Breeding, Key Laboratory of Genome Editing Research and Application (MARA), National Maize Improvement Center, China Agricultural University, Beijing 100193, P.R. China; Sanya International Maize Research Center, Sanya Institute of China Agricultural University, Sanya 572025, P.R. China; Center for Crop Functional Genomics and Molecular Breeding, China Agricultural University, Beijing 100193, P.R. China. Electronic address: jlai@cau.edu.cn. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Cell JT - Cell JID - 0413066 SB - IM OTO - NOTNLM OT - adaptive selection OT - estimation OT - heritability OT - maize OT - population genetics OT - rare variants OT - reference panel OT - whole-genome sequencing COIS- Declaration of interests The authors declare no conflicts of interest. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:42 PHST- 2026/03/13 00:00 [received] PHST- 2026/07/14 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:42 [entrez] AID - S0092-8674(26)01209-2 [pii] AID - 10.1016/j.cell.2026.09.063 [doi] PST - aheadofprint SO - Cell. 2026 Oct 27:S0092-8674(26)01209-2. doi: 10.1016/j.cell.2026.09.063. PMID- 42945951 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1740-0929 (Electronic) IS - 1344-3941 (Linking) VI - 97 IP - 1 DP - 2026 Jan-Dec TI - Maternal Supplementation With Rumen-Protected Methionine and Trace Minerals During Lactation Improves Growth Performance and Increases the Abundance of Fiber-Degrading Bacteria in Yak Calves. LID - e70252 [pii] LID - 10.1111/asj.70252 [doi] AB - This study was conducted to investigate the effects of trace minerals (TM) and rumen-protected methionine (RPM) maternal supplementation on growth performance and rumen bacteria in yak calves. Six 3-month-old yak calves (bodyweight: 38.5 +/- 3 kg) were selected from each maternal diet group (control [CON], TM, and RPM group) and named as follows: CON-YC (CON group yak calves), TM-YC (TM group yak calves), and RPM-YC (RPM group yak calves). Yaks in the RPM group showed greater milk protein and omega-6 polyunsaturated fatty acid contents than those in the CON group (p = 0.01). The TM-YC and RPM-YC groups showed higher (p = 0.04) average daily gain (ADG) and a lower (p < 0.01) incidence of diarrhea than the CON-YC. Concentrations of propionate and butyrate in the rumen were higher in the RPM-YC group than in the CON-YC and TM-YC groups (p < 0.05). Rumen bacteria composition analysis revealed that the abundance of Firmicutes was augmented in the RPM-YC group, with a greater relative abundance of Ruminococcaceae_NK4A214_group than the CON-YC group (p < 0.05). These results indicate that supplementation of lactating yaks with TM and RPM increased ADG and fiber-degrading bacteria abundance in calves while decreasing the incidence of diarrhea. CI - (c) 2026 Japanese Society of Animal Science. FAU - Zhao, Zhiwei AU - Zhao Z AUID- ORCID: 0009-0003-6134-5213 AD - State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University, Lanzhou, China. AD - State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China. AD - Engineering Research Center of Grassland Industry, Ministry of Education, Lanzhou University, Lanzhou, China. AD - Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou University, Lanzhou, China. FAU - Ma, Zhiyuan AU - Ma Z AD - State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Ecology, Lanzhou University, Lanzhou, China. AD - State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China. AD - Engineering Research Center of Grassland Industry, Ministry of Education, Lanzhou University, Lanzhou, China. AD - Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou University, Lanzhou, China. FAU - Wang, Hucheng AU - Wang H AUID- ORCID: 0000-0003-2741-6084 AD - State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, China. AD - Engineering Research Center of Grassland Industry, Ministry of Education, Lanzhou University, Lanzhou, China. AD - Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou University, Lanzhou, China. LA - eng GR - (24)1436/Sichuan Province Aba Prefecture Government Procurement Project/ PT - Journal Article PL - Australia TA - Anim Sci J JT - Animal science journal = Nihon chikusan Gakkaiho JID - 100956805 RN - AE28F7PNPL (Methionine) RN - 0 (Trace Elements) RN - 0 (Dietary Fiber) RN - 0 (Butyrates) RN - 0 (Propionates) SB - IM MH - Animals MH - Cattle/growth & development/microbiology/metabolism MH - *Methionine/administration & dosage/pharmacology MH - *Rumen/microbiology/metabolism MH - *Lactation/physiology MH - Female MH - *Dietary Supplements MH - *Trace Elements/administration & dosage MH - *Diet/veterinary MH - *Animal Nutritional Physiological Phenomena/physiology MH - *Dietary Fiber/metabolism MH - *Animal Feed MH - Butyrates/metabolism MH - Propionates/metabolism MH - *Bacillota OTO - NOTNLM OT - rumen bacteria OT - rumen-protected methionine OT - trace minerals OT - yak calves EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 CRDT- 2026/10/27 19:13 PHST- 2026/09/27 00:00 [revised] PHST- 2026/04/29 00:00 [received] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:13 [entrez] AID - e70252 [pii] AID - 10.1111/asj.70252 [doi] PST - ppublish SO - Anim Sci J. 2026 Jan-Dec;97(1):e70252. doi: 10.1111/asj.70252. PMID- 42945946 OWN - NLM STAT- Publisher LR - 20261027 IS - 1878-5905 (Electronic) IS - 0142-9612 (Linking) VI - 339 DP - 2026 Oct 21 TI - Cell-enriched granular bioinks enable vascular integration and endometrial repair. PG - 124709 LID - S0142-9612(26)00733-7 [pii] LID - 10.1016/j.biomaterials.2026.124709 [doi] AB - 3D bioprinting is essential for constructing functional tissue mimics but remains challenging due to the difficulty of simultaneously achieving adequate cell-cell interactions and appropriate biomaterial support. Here, we introduce a cell-driven clustering strategy that assembles hydrogel microparticles into cell-rich aggregates, in which cells occupy the interstitial spaces and establish pre-organized cellular interactions prior to printing. The cell volume fraction and aggregate size are tunable by microparticle properties and cell type. When applied to digital light processing 3D bioprinting, the cell-clustered granular bioink exhibits high printability while maintaining high cell density and viability. Importantly, preserving pre-established cellular organization translates into enhanced function, including improved stemness maintenance and a more regenerative paracrine profile characterized by increased angiogenic factor secretion and reduced inflammatory signaling. In vivo, implanted constructs show improved cell retention, successful vascular integration with host circulation, and significantly enhanced endometrial repair with reduced fibrosis. These results establish cell-clustered granular bioinks as a generalizable biofabrication strategy for engineering transplantable artificial tissues. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Zhao, Fanxuan AU - Zhao F AD - Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China. FAU - Jin, Shuiling AU - Jin S AD - College of Biomedical Engineering & Instrument Science, Key Laboratory for Biomedical Engineering of Ministry of Education, Zhejiang University, Hangzhou, 310027, China; National Engineering Research Center of Innovation and Application of Minimally Invasive Instruments, Zhejiang University, Hangzhou, 310016, China. FAU - Liu, Yuqi AU - Liu Y AD - Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China. FAU - Zeng, Biya AU - Zeng B AD - Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China. FAU - Gu, Weijia AU - Gu W AD - Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China. FAU - Ye, Xuesong AU - Ye X AD - College of Biomedical Engineering & Instrument Science, Key Laboratory for Biomedical Engineering of Ministry of Education, Zhejiang University, Hangzhou, 310027, China; National Engineering Research Center of Innovation and Application of Minimally Invasive Instruments, Zhejiang University, Hangzhou, 310016, China. FAU - Dai, Yongdong AU - Dai Y AD - Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China. Electronic address: daiyongdong@zju.edu.cn. FAU - Xin, Shangjing AU - Xin S AD - College of Biomedical Engineering & Instrument Science, Key Laboratory for Biomedical Engineering of Ministry of Education, Zhejiang University, Hangzhou, 310027, China; National Engineering Research Center of Innovation and Application of Minimally Invasive Instruments, Zhejiang University, Hangzhou, 310016, China; Zhejiang Key Laboratory of Clinical Evaluation Technology for Medical Device, Zhejiang University, Hangzhou, 310027, China. Electronic address: xinshangjing@zju.edu.cn. FAU - Zhang, Songying AU - Zhang S AD - Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310016, China. Electronic address: zhangsongying@zju.edu.cn. LA - eng PT - Journal Article DEP - 20261021 PL - Netherlands TA - Biomaterials JT - Biomaterials JID - 8100316 SB - IM OTO - NOTNLM OT - 3D bioprinting OT - Cell-cell interactions OT - Endometrial repair OT - Granular bioinks OT - Hydrogel microparticles OT - Tissue integration COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:13 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:13 [entrez] AID - S0142-9612(26)00733-7 [pii] AID - 10.1016/j.biomaterials.2026.124709 [doi] PST - aheadofprint SO - Biomaterials. 2026 Oct 21;339:124709. doi: 10.1016/j.biomaterials.2026.124709. PMID- 42945945 OWN - NLM STAT- Publisher LR - 20261027 IS - 1878-5905 (Electronic) IS - 0142-9612 (Linking) VI - 339 DP - 2026 Oct 26 TI - An injectable ROS-responsive natural drug self-delivery hydrogel relieves neuropathic pain by remodeling DRG macrophage-mediated neuroinflammation. PG - 124723 LID - S0142-9612(26)00747-7 [pii] LID - 10.1016/j.biomaterials.2026.124723 [doi] AB - Neuropathic pain remains a major clinical challenge owing to complex neuroimmune mechanisms and limited disease-modifying therapies. Neuroinflammation within the dorsal root ganglion (DRG), particularly macrophage infiltration and pro-inflammatory polarization, is a key driver of sensory neuronal sensitization and pain persistence. Here, we report an injectable reactive oxygen species (ROS)-responsive natural drug self-delivery hydrogel for localized and sustained treatment of neuropathic pain. Single-cell transcriptomic reanalysis revealed marked macrophage accumulation in injured DRGs, accompanied by M1-like polarization and aberrant phosphatidylinositol 3-kinase (PI3K)-Akt activation. Mechanism-guided virtual screening identified glycyrrhizic acid (GA), a bioactive natural product, as a promising PI3K-targeting candidate. To improve local bioavailability, GA was engineered into a 1,4-phenylenediboronic acid (1,4-PBA)-functionalized self-assembled hydrogel, termed GA-PBA-GA (GPG), through dynamic boronate ester bonds and non-covalent interactions. The GPG hydrogel exhibited injectability, self-healing, biodegradability, biocompatibility, and inflammation-associated ROS-triggered GA release. In vitro, GPG suppressed PI3K-Akt signaling, reduced pro-inflammatory macrophage activation, and promoted M1-to-M2 repolarization. In a rat model of L5 spinal nerve ligation-induced neuropathic pain, peri-DRG administration alleviated mechanical and thermal hypersensitivity, improved gait and electrophysiological function, reduced DRG macrophage-mediated neuroinflammation, and attenuated spinal microglial activation. This ROS-responsive natural drug self-delivery hydrogel provides a minimally invasive strategy for remodeling DRG neuroinflammation and achieving sustained relief of neuropathic pain through localized drug delivery. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Xing, Xudan AU - Xing X AD - Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, Department of Orthopaedic Surgery, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, 510150, China; Department of Anesthesiology, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610000, China. FAU - Yang, Ge AU - Yang G AD - Orthopedic Department, Hunan Provincial Key Laboratory of Pediatric Orthopedics, Hunan Children's Hospital, Changsha, 410007, China. FAU - Liu, Peiyi AU - Liu P AD - Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, Department of Orthopaedic Surgery, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, 510150, China. FAU - Chen, Chuke AU - Chen C AD - Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, Department of Orthopaedic Surgery, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, 510150, China. FAU - Gao, Hongao AU - Gao H AD - Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, Department of Orthopaedic Surgery, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, 510150, China. FAU - Li, Jiajun AU - Li J AD - Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, Department of Orthopaedic Surgery, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, 510150, China. FAU - Yu, Hui AU - Yu H AD - Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, Department of Orthopaedic Surgery, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, 510150, China. FAU - Feng, Yuan AU - Feng Y AD - Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, Department of Orthopaedic Surgery, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, 510150, China. FAU - Jiang, Guiyong AU - Jiang G AD - Division of Orthopaedics and Traumatology, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China. Electronic address: nfyy_jgy@163.com. FAU - Wang, Le AU - Wang L AD - Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, Department of Orthopaedic Surgery, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, 510150, China. Electronic address: wangle@gzhmu.edu.cn. FAU - Li, Peng AU - Li P AD - Department of Anesthesiology, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610000, China. Electronic address: lipengmazui@163.com. FAU - Zhou, Lei AU - Zhou L AD - Guangzhou Key Laboratory of Spine Disease Prevention and Treatment, Department of Orthopaedic Surgery, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, 510150, China. Electronic address: zhoul@gzhmu.edu.cn. LA - eng PT - Journal Article DEP - 20261026 PL - Netherlands TA - Biomaterials JT - Biomaterials JID - 8100316 SB - IM OTO - NOTNLM OT - Dorsal root ganglion OT - Macrophage polarization OT - Neuropathic pain OT - PI3K-Akt signaling OT - Responsive drug release OT - Self-assembling hydrogel COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:13 PHST- 2026/07/17 00:00 [received] PHST- 2026/10/21 00:00 [revised] PHST- 2026/10/24 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:13 [entrez] AID - S0142-9612(26)00747-7 [pii] AID - 10.1016/j.biomaterials.2026.124723 [doi] PST - aheadofprint SO - Biomaterials. 2026 Oct 26;339:124723. doi: 10.1016/j.biomaterials.2026.124723. PMID- 42945942 OWN - NLM STAT- Publisher LR - 20261027 IS - 1879-1883 (Electronic) IS - 0002-9610 (Linking) VI - 263 DP - 2026 Oct 16 TI - Increased risk of hypocalcemia and RLN injury in patients with medullary thyroid carcinoma after thyroidectomy. PG - 117296 LID - S0002-9610(26)00481-2 [pii] LID - 10.1016/j.amjsurg.2026.117296 [doi] AB - INTRODUCTION: In general, patients with medullary thyroid carcinoma (MTC) undergo total thyroidectomy and central neck dissection (CND), whereas only select patients with differentiated thyroid carcinoma (DTC) receive CND. METHODS: The ACS NSQIP targeted thyroidectomy dataset (2016-2024) was queried for adults with MTC or DTC undergoing total thyroidectomy. Rates of CND, lateral neck dissection (LND) and postoperative complications were compared. RESULTS: Among 23,337 patients, 444 (1.9%) had MTC and 22,893 (98.1%) had DTC. CND was performed more frequently in MTC than DTC (72.1% vs 40.1%, p < 0.01). MTC patients had higher rates of hypocalcemia within 30 days (11.5% vs 6.8%, p < 0.01) and recurrent laryngeal nerve (RLN) injury/dysfunction (10.4% vs 6.4%, p < 0.01). After adjustment for CND and LND, MTC remained associated with hypocalcemia (OR 1.44, p = 0.03). CONCLUSION: Patients with MTC have higher rates of post-operative hypocalcemia and RLN injury than those with DTC. MTC remained independently associated with hypocalcemia within 30 days even after accounting for differences in operative extent. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Londono, Evelyn AU - Londono E AD - Temple University Hospital, Department of General Surgery, 3401 N. Broad St., Zone C, 4th Floor, Philadelphia, PA, 19140, USA. Electronic address: evelyn.londono@tuhs.temple.edu. FAU - Green, Rebecca AU - Green R AD - Temple University Hospital, Department of General Surgery, 3401 N. Broad St., Zone C, 4th Floor, Philadelphia, PA, 19140, USA. FAU - HoSang, Kristen AU - HoSang K AD - Temple University Hospital, Department of General Surgery, 3401 N. Broad St., Zone C, 4th Floor, Philadelphia, PA, 19140, USA. FAU - Gao, Terry P AU - Gao TP AD - Temple University Hospital, Department of General Surgery, 3401 N. Broad St., Zone C, 4th Floor, Philadelphia, PA, 19140, USA. FAU - Kuo, Lindsay E AU - Kuo LE AD - Temple University Hospital, Department of General Surgery, 3401 N. Broad St., Zone C, 4th Floor, Philadelphia, PA, 19140, USA. LA - eng PT - Journal Article DEP - 20261016 PL - United States TA - Am J Surg JT - American journal of surgery JID - 0370473 SB - IM OTO - NOTNLM OT - Central neck dissection OT - Hypocalcemia OT - Medullary thyroid carcinoma OT - Recurrent laryngeal nerve injury OT - Thyroidectomy COIS- Declaration of competing interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:13 PHST- 2026/07/12 00:00 [received] PHST- 2026/09/07 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:13 [entrez] AID - S0002-9610(26)00481-2 [pii] AID - 10.1016/j.amjsurg.2026.117296 [doi] PST - aheadofprint SO - Am J Surg. 2026 Oct 16;263:117296. doi: 10.1016/j.amjsurg.2026.117296. PMID- 42945935 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-3379 (Electronic) IS - 0891-4222 (Linking) VI - 178 DP - 2026 Oct 27 TI - The ARIEL Visual Search Task (AVST): Preliminary psychometric evaluation of a novel measure of selective visual attention in autistic adolescents. PG - 105407 LID - S0891-4222(26)00202-7 [pii] LID - 10.1016/j.ridd.2026.105407 [doi] AB - BACKGROUND: Selective visual attention is relevant to goal-directed behavior, yet visual-search measures vary in linguistic demands, stimulus construction, and methodological transparency. The aim of the current study was to develop the ARIEL Visual Search Task (AVST), a minimally linguistic performance-based measure of selective visual attention, and evaluate its feasibility, performance characteristics, reliability, convergent validity, and known-groups differentiation in autistic adolescents. METHODS: The AVST was developed using explicit rules for symbol construction, target-distractor similarity, form assembly, administration, and scoring. The study enrolled 40 autistic adolescents (mean age = 13.6 years, SD = 1.8) and 25 non-autistic adolescents (mean age = 13.4 years, SD = 1.7). Thirty-eight autistic participants completed Form A and contributed to the primary performance analyses. Data were analyzed to examine feasibility, score distributions, item and distractor performance, internal consistency, and known-groups differences. Sub-samples were used to examine test-retest reliability and convergent validity with the CEFT, while distractor classification was independently evaluated through expert review and a separate perceptual-evaluation sample. RESULTS: Thirty-eight autistic participants (95.0%) completed the AVST, with a mean completion time of 3.5 min (SD = 1.1). Mean Hits were 10.03 of 12 (SD = 1.8), and mean False Alarms were 4.3 (SD = 3.1). Item detection rates ranged from 65.8% to 94.7%, and internal consistency of the 12 target-detection items was KR-20 = .58. Preliminary test-retest ICCs ranged from.58 to.82, and AVST Hits and False Alarms showed modest associations with CEFT accuracy (rho =.43 and -.38, respectively). Non-autistic participants showed higher Hits (|d| = 0.84, p < .001) and fewer False Alarms (|d| = 0.83, p = .001). Completion Time also differed moderately between groups (d = 0.60, p = .015), whereas the difference in Self-Corrections was smaller and not statistically significant (d = 0.43, p = .078). Distractor-related findings were broadly consistent with the predefined similarity framework. CONCLUSION: The AVST was feasible to administer in autistic adolescents and showed promising initial measurement properties, particularly for target detection, false selections, and completion time. These findings support further evaluation of the task's reliability and validity in larger and more diverse samples. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Alothman, Abdulaziz Abdullah AU - Alothman AA AD - Department of Special Education, College of Education, Majmaah University, Majmaah, 11952, Saudi Arabia; King Salman Center for Disability Research, Riyadh, 11614, Saudi Arabia. Electronic address: aa.alothman@mu.edu.sa. FAU - Didden, Robert AU - Didden R AD - Behavioural Science Institute, Radboud University, Nijmegen, the Netherlands. FAU - Ebrahim, Mona Tawakkul AU - Ebrahim MT AD - Department of Educational Sciences, College of Education, Majmaah University, Majmaah, 11952, Saudi Arabia; King Salman Center for Disability Research, Riyadh, 11614, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Res Dev Disabil JT - Research in developmental disabilities JID - 8709782 SB - IM OTO - NOTNLM OT - Adolescents OT - Autism OT - Instrument development OT - Psychometric evaluation OT - Selective visual attention OT - Visual search COIS- Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Abdulaziz Abdullah Alothman reports financial support was provided by King Salman Center for Disability Research. Robert Didden serves as an Associate Editor of Research in Developmental Disabilities and had no involvement in the peer review or editorial decision for this article. The remaining author declares no competing interests. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:13 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:13 [entrez] AID - S0891-4222(26)00202-7 [pii] AID - 10.1016/j.ridd.2026.105407 [doi] PST - aheadofprint SO - Res Dev Disabil. 2026 Oct 27;178:105407. doi: 10.1016/j.ridd.2026.105407. PMID- 42945933 OWN - NLM STAT- Publisher LR - 20261027 IS - 1532-7361 (Electronic) IS - 0039-6060 (Linking) VI - 202 DP - 2026 Sep 25 TI - Body mass index and textbook outcome after robotic liver resection: A multinational cohort study across 30 centers. PG - 110708 LID - S0039-6060(26)00633-1 [pii] LID - 10.1016/j.surg.2026.110708 [doi] AB - BACKGROUND: Obesity is associated with worse outcomes after open and laparoscopic liver resection, but its independent contribution after robotic liver resection has not been formally interrogated in a large cohort. This study aims to determine whether body mass index predicts textbook outcome after robotic liver resection. METHODS: Consecutive adult patients undergoing robotic liver resection at 30 international high-volume benchmark centers (2020-2023) were analyzed. Living donor hepatectomies were excluded from the primary analysis. The primary outcome was textbook outcome. Generalized estimating equations with center as the cluster provided cluster-robust standard errors for all multivariable models. Sensitivity analyses reincluded living donors, applied Asia-Pacific body mass index cut points, and restricted to high-contributing centers. RESULTS: Among 2,905 nondonor patients (normal: 1,263; overweight: 1,007; obese: 427; and morbidly obese: 208) across 29 centers, the overall non-textbook outcome rate was 22.0% and differed little across body mass index strata unadjusted (19.6%, 24.2%, 23.0%, and 23.6%; P = .05). Center-level variation was substantial. After adjustment, body mass index was not an independent predictor (per 1 kg/m(2), odds ratio, 1.00; 95% confidence interval, 0.98-1.02; P = .81; morbidly obese versus normal: odds ratio, 1.01; 95% confidence interval, 0.68-1.51; P = .96), and adding body mass index to the multivariable model left discrimination unchanged (area under the curve, 0.699 with and without body mass index). Dominant determinants of non-textbook outcome were malignant indication (odds ratio, 2.45, 1.70-3.52; P < .001), major hepatectomy (odds ratio, 1.57, 1.24-1.97; P < .001), and neoadjuvant therapy (odds ratio, 1.36; 1.13-1.63; P = .001). Higher body mass index showed a nonsignificant trend toward increased surgical site infection (odds ratio per 5 kg/m(2,) 1.18, 0.99-1.41; P = .06) but no association with major complications. Findings were robust across all sensitivity analyses, including reinclusion of the 1,180 living donors. No body mass index x IWATE interaction was detected (P = .82). CONCLUSION: Among patients selected for robotic liver resection at experienced centers, body mass index was not an independent determinant of textbook outcome or of major complications. Surgical complexity, indication, and extent of resection were the dominant drivers of perioperative outcome. These findings are conditional on a robotic approach having been offered and do not address the upstream choice between robotic, laparoscopic, and open surgery. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Li, Zhihao AU - Li Z AD - Department of Surgery and Transplantation, University of Zurich, Zurich, Switzerland; Division of Transplant Surgery, The William J. Von Liebig Center for Transplantation and Clinical Regeneration, Mayo Clinic, Rochester, MN. FAU - Raptis, Dimitri Aristotle AU - Raptis DA AD - Abdominal Transplant and Hepatobiliary-Pancreatic Surgery Center, Organ Transplant Centre of Excellence, King Faisal Specialist Hospital & Research Center, Riyadh, Saudi Arabia. FAU - Ma, Yong AU - Ma Y AD - Department of Minimally Invasive Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China. FAU - Yang, Guangchao AU - Yang G AD - Department of Minimally Invasive Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China. FAU - Li, Linqiang AU - Li L AD - Department of Minimally Invasive Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China. FAU - Song, Xuan AU - Song X AD - Department of Minimally Invasive Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, People's Republic of China. FAU - Millet, Guillaume AU - Millet G AD - Digestive Surgery and Transplantation Department, CHU de Lille, University of Lille, Lille, France. FAU - Truant, Stephanie AU - Truant S AD - Digestive Surgery and Transplantation Department, CHU de Lille, University of Lille, Lille, France. FAU - Venezia, Raphael AU - Venezia R AD - Universite Paris-Est Creteil, UPEC, Creteil, France; INSERM, U955, Team "Virus Hepatologie Cancer", Creteil, France; Department of Digestive and Hepatobiliary Surgery, Assistance Publique-Hopitaux de Paris, Henri Mondor University Hospital, Creteil, France. FAU - Jeddou, Heithem AU - Jeddou H AD - Department of Hepatobiliary and Digestive Surgery, Pontchaillou University Hospital, Rennes 1 University, Rennes, France. FAU - Le Floch, Bastien AU - Le Floch B AD - Department of Hepatobiliary and Digestive Surgery, Pontchaillou University Hospital, Rennes 1 University, Rennes, France. FAU - Valverde, Alain AU - Valverde A AD - Department of Digestive Surgery, Groupe Hospitalier Diaconesses Croix Saint Simon, Paris, France. FAU - Peru, Nicolas AU - Peru N AD - Department of Digestive Surgery, Groupe Hospitalier Diaconesses Croix Saint Simon, Paris, France. FAU - Pessaux, Patrick AU - Pessaux P AD - Department of Digestive Surgery, Strasbourg University Hospital, INSERM U1110, Strasbourg, France. FAU - Giannone, Fabio AU - Giannone F AD - Department of Digestive Surgery, Strasbourg University Hospital, INSERM U1110, Strasbourg, France; Center for Advanced Treatment of Hepatobiliary and Pancreatic Diseases, Ageo Central General Hospital, Saitama, Japan. FAU - Mabrut, Jean Yves AU - Mabrut JY AD - Department of Hepatobiliopancreatic Surgery and Liver Transplantation, Hospital Croix Rousse, Lyon, France. FAU - Mohkam, Kayvan AU - Mohkam K AD - Department of Hepatobiliopancreatic Surgery and Liver Transplantation, Hospital Croix Rousse, Lyon, France. FAU - Cunha, Antonio Sa AU - Cunha AS AD - Hepato-Biliary Center, Paul Brousse, AP-HP, Villejuif, Paris, France. FAU - Salloum, Chady AU - Salloum C AD - Hepato-Biliary Center, Paul Brousse, AP-HP, Villejuif, Paris, France. FAU - Blanc, Pierre Yves AU - Blanc PY AD - Department of Visceral and Digestive Surgery, Center Hospitalier Universitaire de Saint-Etienne, Saint-Etienne, France. FAU - Le Roy, Bertrand AU - Le Roy B AD - Department of Visceral and Digestive Surgery, Center Hospitalier Universitaire de Saint-Etienne, Saint-Etienne, France. FAU - Goumard, Claire AU - Goumard C AD - Department of Hepatobiliary Surgery and Liver Transplantation, Sorbonne University, Hopital Pitie-Salpetriere, Assistance Publique- Hopitaux de Paris, Paris, France. FAU - Scatton, Olivier AU - Scatton O AD - Department of Hepatobiliary Surgery and Liver Transplantation, Sorbonne University, Hopital Pitie-Salpetriere, Assistance Publique- Hopitaux de Paris, Paris, France. FAU - Tzedakis, Stylianos AU - Tzedakis S AD - Department of Hepatobiliary, Digestive and Endocrine Surgery, Universite Paris Cite, Cochin Hospital, AP-HP, Paris, France. FAU - Fuks, David AU - Fuks D AD - Department of Hepatobiliary, Digestive and Endocrine Surgery, Universite Paris Cite, Cochin Hospital, AP-HP, Paris, France. FAU - Chaoui, Ismael AU - Chaoui I AD - Department of Digestive and Hepatobiliary/Pancreatic Surgery, Groeninge Hospital, Kortrijk, Belgium. FAU - Rammohan, Ashwin AU - Rammohan A AD - The Institute of Liver Disease & Transplantation, Dr Rela Institute and Medical Center, Chennai, India. FAU - Gomes da Silva, Silvia AU - Gomes da Silva S AD - Hepato-Biliary-Pancreatic and Transplantation Center, Centro Hospitalar Universitario de Lisboa Central, Lisbon, Portugal. FAU - Nunes Sobral, Mafalda Sampaio AU - Nunes Sobral MS AD - Hepato-Biliary-Pancreatic and Transplantation Center, Centro Hospitalar Universitario de Lisboa Central, Lisbon, Portugal. FAU - Lopez-Lopez, Victor AU - Lopez-Lopez V AD - Department of General, Visceral and Transplantation Surgery, Clinic and University Hospital Virgen de la Arrixaca, Murcia, Spain. FAU - Centonze, Leonardo AU - Centonze L AD - Department of General Surgery and Transplantation, Niguarda Ca' Granda Hospital, Milan, Italy. FAU - Zhu, Zhu AU - Zhu Z AD - Department of Hepatopancreatobiliary Surgery, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, China. FAU - Ratti, Francesca AU - Ratti F AD - Division of Hepatobiliary Surgery, IRCCS San Raffaele Hospital, Vita-Salute University, Milan, Italy. FAU - Marino, Rebecca AU - Marino R AD - Division of Hepatobiliary Surgery, IRCCS San Raffaele Hospital, Vita-Salute University, Milan, Italy. FAU - Rompianesi, Gianluca AU - Rompianesi G AD - Division of HPB, Minimally Invasive and Robotic Surgery, Federico II University Hospital Naples, Naples, Italy. FAU - Araujo, Raphael L C AU - Araujo RLC AD - Division of HPB Surgery, Carolinas Medical Center, Atrium Health, Charlotte. FAU - Dong, Yawen AU - Dong Y AD - Department of Hepatobiliary and Pancreas Surgery, Mayo Clinic Rochester, Rochester. FAU - Hardy, Niall AU - Hardy N AD - Department of Hepatobiliary and Pancreas Surgery, Mayo Clinic Rochester, Rochester. FAU - Shah, Jimmy AU - Shah J AD - The Liver Institute, Methodist Dallas Medical Center, Dallas. FAU - Muller, Philip C AU - Muller PC AD - Department of Surgery, Clarunis University Center for Gastrointestinal and Liver Diseases, Basel, Switzerland; Department of Visceral Surgery, University Hospital Basel, Basel, Switzerland. FAU - Wakabayashi, Taiga AU - Wakabayashi T AD - Japanese Foundation for Cancer Research, Division of Hepatobiliary and Pancreatic Surgery, Cancer Institute Hospital, Tokyo, Japan. FAU - Coelho, Fabricio Ferreira AU - Coelho FF AD - Department of Surgery, Hospital das Clinicas, University of Sao Paulo Medical School, Sao Paulo, Brazil. FAU - Castillo-Flores, Samy AU - Castillo-Flores S AD - Department of Surgery, University of Texas Southwestern Medical Center, Dallas. FAU - Lee, Boram AU - Lee B AD - Department of Surgery, Seoul National University Hospital Bundang, Seoul National University College of Medicine, Seoul, South Korea. FAU - Viti, Virginia AU - Viti V AD - Division of General and Transplant Surgery, University of Pisa, Pisa, Italy. FAU - Kato, Yutaro AU - Kato Y AD - Department of Gastroenterological Surgery, Bantane Hospital, Fujita Health University, Nagoya, Japan. FAU - Boggi, Ugo AU - Boggi U AD - Division of General and Transplant Surgery, University of Pisa, Pisa, Italy. FAU - Han, Ho-Seong AU - Han HS AD - Department of Surgery, Seoul National University Hospital Bundang, Seoul National University College of Medicine, Seoul, South Korea. FAU - Polanco, Patricio AU - Polanco P AD - Department of Surgery, University of Texas Southwestern Medical Center, Dallas. FAU - Goh, Brian K P AU - Goh BKP AD - Department of Hepatopancreatobiliary and Transplant Surgery, Singapore General Hospital, National Cancer Center Singapore, Singapore; Surgery Academic Clinical Program, Duke-National Medical School, Singapore. FAU - Cheung, Tan-To AU - Cheung TT AD - Department of Surgery, Queen Mary Hospital, The University of Hong Kong, Hong Kong SAR, China. FAU - Efanov, Mikhail AU - Efanov M AD - Department of Hepato-Pancreato-Biliary Surgery, Moscow Clinical Scientific Center, Moscow, Russia. FAU - Panaro, Fabrizio AU - Panaro F AD - SCDU General Surgery, Surgical Oncology, Minimally Invasive, Robotic and HBP Surgery, AOUAL SS. Antonio e Biagio e Cesare Arrigo, Alessandria, Italy. FAU - Machado, Marcel AU - Machado M AD - Department of Surgery, Hospital das Clinicas, University of Sao Paulo Medical School, Sao Paulo, Brazil. FAU - Herman, Paulo AU - Herman P AD - Department of Surgery, Hospital das Clinicas, University of Sao Paulo Medical School, Sao Paulo, Brazil. FAU - Wakabayashi, Go AU - Wakabayashi G AD - Center for Advanced Treatment of Hepatobiliary and Pancreatic Diseases, Ageo Central General Hospital, Saitama, Japan. FAU - Ielpo, Benedetto AU - Ielpo B AD - HPB Unit, Hospital del mar, Barcelona, Spain. FAU - Muller, Beat P AU - Muller BP AD - Department of Surgery, Clarunis University Center for Gastrointestinal and Liver Diseases, Basel, Switzerland; Department of Visceral Surgery, University Hospital Basel, Basel, Switzerland. FAU - Mejia, Alejandro AU - Mejia A AD - The Liver Institute, Methodist Dallas Medical Center, Dallas. FAU - Starlinger, Patrick AU - Starlinger P AD - Department of Hepatobiliary and Pancreas Surgery, Mayo Clinic Rochester, Rochester. FAU - Martinie, John AU - Martinie J AD - Division of HPB Surgery, Carolinas Medical Center, Atrium Health, Charlotte. FAU - Troisi, Roberto Ivan AU - Troisi RI AD - Division of HPB, Minimally Invasive and Robotic Surgery, Federico II University Hospital Naples, Naples, Italy. FAU - Chen, Guodong AU - Chen G AD - Department of Hepatopancreatobiliary Surgery, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, China. FAU - Di Sandro, Stefano AU - Di Sandro S AD - Department of General Surgery and Transplantation, Niguarda Ca' Granda Hospital, Milan, Italy. FAU - Robles-Campos, Ricardo AU - Robles-Campos R AD - Department of General, Visceral and Transplantation Surgery, Clinic and University Hospital Virgen de la Arrixaca, Murcia, Spain. FAU - Choi, Gi Hong AU - Choi GH AD - Division of Hepatopancreatobiliary Surgery, Department of Surgery, Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea. FAU - Liu, Rong AU - Liu R AD - Faculty of Hepatopancreatobiliary Surgery, The First Medical Center of Chinese People's Liberation Army (PLA) General Hospital, Beijing, China. FAU - Di Benedetto, Fabrizio AU - Di Benedetto F AD - Hepato-Pancreato-Biliary Surgery and Liver Transplantation Unit, University of Modena and Reggio Emilia, Modena, Italy. FAU - Marques, Hugo Pinto AU - Marques HP AD - Hepato-Biliary-Pancreatic and Transplantation Center, Centro Hospitalar Universitario de Lisboa Central, Lisbon, Portugal. FAU - Sucandy, Iswanto AU - Sucandy I AD - Digestive Health Institute, AdventHealth Tampa, FL. FAU - Rela, Mohamed AU - Rela M AD - The Institute of Liver Disease & Transplantation, Dr Rela Institute and Medical Center, Chennai, India. FAU - D'Hondt, Mathieu AU - D'Hondt M AD - Department of Digestive and Hepatobiliary/Pancreatic Surgery, Groeninge Hospital, Kortrijk, Belgium. FAU - Brustia, Raffaele AU - Brustia R AD - Universite Paris-Est Creteil, UPEC, Creteil, France; INSERM, U955, Team "Virus Hepatologie Cancer", Creteil, France; Department of Digestive and Hepatobiliary Surgery, Assistance Publique-Hopitaux de Paris, Henri Mondor University Hospital, Creteil, France. FAU - Soubrane, Olivier AU - Soubrane O AD - Department of Digestive, Oncological and Metabolic Surgery, Institut Mutualiste Montsouris, Paris, France. FAU - Broering, Dieter Clemens AU - Broering DC AD - Abdominal Transplant and Hepatobiliary-Pancreatic Surgery Center, Organ Transplant Centre of Excellence, King Faisal Specialist Hospital & Research Center, Riyadh, Saudi Arabia. FAU - Clavien, Pierre-Alain AU - Clavien PA AD - Department of Surgery and Transplantation, University of Zurich, Zurich, Switzerland; Wyss Zurich Translational Center, ETH Zurich and University of Zurich, Zurich, Switzerland. Electronic address: clavien@access.uzh.ch. LA - eng PT - Journal Article DEP - 20260925 PL - United States TA - Surgery JT - Surgery JID - 0417347 SB - IM COIS- Conflict of Interest/Disclosure The authors declare no conflicts of interest related to this work. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:13 PHST- 2026/06/09 00:00 [received] PHST- 2026/08/24 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:13 [entrez] AID - S0039-6060(26)00633-1 [pii] AID - 10.1016/j.surg.2026.110708 [doi] PST - aheadofprint SO - Surgery. 2026 Sep 25;202:110708. doi: 10.1016/j.surg.2026.110708. PMID- 42945926 OWN - NLM STAT- Publisher LR - 20261027 IS - 1532-7361 (Electronic) IS - 0039-6060 (Linking) VI - 202 DP - 2026 Oct 1 TI - Toward risk-adapted surgical de-escalation in sporadic medullary thyroid carcinoma: Integrating desmoplastic stromal reaction, nodal status, and multifocality. PG - 110732 LID - S0039-6060(26)00657-4 [pii] LID - 10.1016/j.surg.2026.110732 [doi] AB - BACKGROUND: Risk-adapted de-escalation in sporadic medullary thyroid carcinoma requires identification of patients in whom lymph-node dissection and thyroid preservation can be safely modulated. Desmoplastic stromal reaction predicts nodal metastases, but whether desmoplastic stromal reaction, nodal status, and multifocality can be integrated into a staged clinical decision framework remains unclear. METHODS: This retrospective cohort study included adults with sporadic medullary thyroid carcinoma undergoing index surgery between January 2018 and March 2026 at a referral endocrine surgery center. Factors associated with lymph node metastases, multifocality, and the pN0-unifocal phenotype were assessed using univariable analyses and exploratory machine-learning models. RESULTS: Among 331 patients operated for medullary thyroid carcinoma, 94 met inclusion criteria. Lymph node metastases were present in 29 patients (30.9%). All node-positive tumors were desmoplastic stromal reaction positive, whereas no desmoplastic stromal reaction-negative tumor had lymph node metastases (P < .001). Lymph node metastases were also associated with higher basal calcitonin and carcinoembryonic antigen, larger tumor diameter, male sex, advanced pT category, high-grade medullary thyroid carcinoma, extrathyroidal extension, vascular invasion, and multifocality. Multifocality occurred in 14 patients (14.9%) and was associated with male sex, advanced pT category, lymph node metastases, and extrathyroidal extension. The pN0-unifocal phenotype was associated with female sex, lower biochemical markers, absence of desmoplastic stromal reaction, smaller tumor diameter, absence of extrathyroidal extension, and absence of vascular invasion. CONCLUSION: Desmoplastic stromal reaction negativity identifies negligible nodal risk but does not exclude occult contralateral disease. We propose a staged clinical decision framework integrating preoperative selection with intraoperative pathologic assessment to explore thyroid preservation in highly selected patients. This framework is hypothesis generating and requires prospective validation. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Pennestri, Francesco AU - Pennestri F AD - UOC Chirurgia Endocrina e Metabolica, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy; Centro di Ricerca in Chirurgia delle Ghiandole Endocrine e dell'Obesita, Universita Cattolica del Sacro Cuore, Rome, Italy. Electronic address: francesco.pennestri@policlinicogemelli.it. FAU - Procopio, Priscilla Francesca AU - Procopio PF AD - UOC Chirurgia Endocrina e Metabolica, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy; Centro di Ricerca in Chirurgia delle Ghiandole Endocrine e dell'Obesita, Universita Cattolica del Sacro Cuore, Rome, Italy. FAU - Santoro, Gloria AU - Santoro G AD - Dipartimento di Scienze Mediche e Chirurgiche Addominali ed Endocrino-Metaboliche, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy. FAU - Laurino, Antonio AU - Laurino A AD - UOC Chirurgia Endocrina e Metabolica, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy. FAU - Parroni, Francesco AU - Parroni F AD - Centro di Ricerca in Chirurgia delle Ghiandole Endocrine e dell'Obesita, Universita Cattolica del Sacro Cuore, Rome, Italy. FAU - Gallucci, Pierpaolo AU - Gallucci P AD - UOC Chirurgia Endocrina e Metabolica, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy. FAU - Prioli, Francesca AU - Prioli F AD - UOC Chirurgia Endocrina e Metabolica, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy. FAU - Rossi, Esther Diana AU - Rossi ED AD - UOC Anatomia Patologica della Testa e Collo, del Polmone e dell'Apparato Endocrino, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy; Dipartimento di Scienza della Vita e Sanita Pubblica, Universita Cattolica del Sacro Cuore, Rome, Italy. FAU - Pontecorvi, Alfredo AU - Pontecorvi A AD - UOC Medicina Interna, Endocrinologia e Diabetologia, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy; Dipartimento di Medicina e Chirurgia Traslazionale, Universita Cattolica del Sacro Cuore, Rome, Italy. FAU - De Crea, Carmela AU - De Crea C AD - UOC Chirurgia Endocrina e Metabolica, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy; Centro di Ricerca in Chirurgia delle Ghiandole Endocrine e dell'Obesita, Universita Cattolica del Sacro Cuore, Rome, Italy. FAU - Raffaelli, Marco AU - Raffaelli M AD - UOC Chirurgia Endocrina e Metabolica, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy; Centro di Ricerca in Chirurgia delle Ghiandole Endocrine e dell'Obesita, Universita Cattolica del Sacro Cuore, Rome, Italy. LA - eng PT - Journal Article DEP - 20261001 PL - United States TA - Surgery JT - Surgery JID - 0417347 SB - IM COIS- Conflict of Interest/Disclosure Marco Raffaelli has consultancy agreements with Medtronic, AB Medica, and Intuitive Surgical. Priscilla Francesca Procopio, Francesco Pennestri, Antonio Laurino, and Pierpaolo Gallucci have consultancy agreements with Medtronic. Gloria Santoro, Francesco Parroni, Francesca Prioli, Esther Diana Rossi, Alfredo Pontecorvi, and Carmela De Crea have no conflicts of interest to disclose. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:13 PHST- 2026/08/10 00:00 [received] PHST- 2026/09/09 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:13 [entrez] AID - S0039-6060(26)00657-4 [pii] AID - 10.1016/j.surg.2026.110732 [doi] PST - aheadofprint SO - Surgery. 2026 Oct 1;202:110732. doi: 10.1016/j.surg.2026.110732. PMID- 42945922 OWN - NLM STAT- Publisher LR - 20261027 IS - 1879-0402 (Electronic) IS - 1367-5931 (Linking) VI - 95 DP - 2026 Oct 27 TI - Non-canonical nucleic acid structures in viruses: Regulatory modules and emerging antiviral targets. PG - 102798 LID - S1367-5931(26)00147-X [pii] LID - 10.1016/j.cbpa.2026.102798 [doi] AB - Beyond their primary sequence, viral genomes contain structural information encoded in the ability of DNA and RNA to fold into alternative conformations. These dynamic non-canonical nucleic acid structures include G-quadruplexes (G4s), i-Motifs (iMs), R-loops, G4-associated R-loops and left-handed Z-conformations. Among them, G4s have been most extensively investigated in viruses and have been reported to modulate transcription, replication, translation, latency and immune evasion. However, the functional interpretation of these structures remains highly context-dependent: similar topologies can either promote or restrict infection depending on genomic location, folding dynamics, protein interactors and the stage of the viral life cycle. Here, we discuss recent advances in viral non-canonical nucleic acid structures, emphasizing the level of evidence supporting their formation and function. We also assess emerging antiviral strategies based on structure-targeting ligands, highlighting both their translational potential and the unresolved challenges of selectivity, target engagement and pharmacological validation in infected cells. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved. FAU - Ruggiero, Emanuela AU - Ruggiero E AD - Department of Molecular Medicine, University of Padova, Via Gabelli, 63, Padova, Italy. FAU - Zanin, Irene AU - Zanin I AD - Department of Molecular Medicine, University of Padova, Via Gabelli, 63, Padova, Italy. FAU - Mattellone, Filippo AU - Mattellone F AD - Department of Molecular Medicine, University of Padova, Via Gabelli, 63, Padova, Italy. FAU - Richter, Sara N AU - Richter SN AD - Department of Molecular Medicine, University of Padova, Via Gabelli, 63, Padova, Italy; Microbiology and Virology Unit, Padua University Hospital, 35121, Padua, Italy. Electronic address: sara.richter@unipd.it. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - England TA - Curr Opin Chem Biol JT - Current opinion in chemical biology JID - 9811312 SB - IM COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:13 PHST- 2026/08/01 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:13 [entrez] AID - S1367-5931(26)00147-X [pii] AID - 10.1016/j.cbpa.2026.102798 [doi] PST - aheadofprint SO - Curr Opin Chem Biol. 2026 Oct 27;95:102798. doi: 10.1016/j.cbpa.2026.102798. PMID- 42945917 OWN - NLM STAT- Publisher LR - 20261027 IS - 1878-1705 (Electronic) IS - 1567-5769 (Linking) VI - 191 IP - Pt 1 DP - 2026 Oct 27 TI - Tetrandrine attenuates diabetic kidney disease by suppressing the TLR4-NF-kappaB-NLRP3 inflammatory signaling pathway in proximal tubular cells. PG - 117612 LID - S1567-5769(26)01460-8 [pii] LID - 10.1016/j.intimp.2026.117612 [doi] AB - BACKGROUND: Diabetic kidney disease (DKD) involves persistent tubular inflammation. Tetrandrine (Tet), a bioactive alkaloid from Stephania tetrandra S. Moore, has renoprotective potential, but its effects on TLR4-associated inflammatory signaling remain incompletely understood. METHODS: Male KK-Ay mice received Tet (10, 20, or 40 mg/kg/day) for 12 weeks. Biochemical parameters, urinary albumin-to-creatinine ratio, renal histopathology, and single-nucleus transcriptomes were assessed. Pathway-associated changes were examined in kidney tissues and palmitic acid (PA)-stimulated HK-2 cells. Molecular docking, molecular dynamics simulation, cellular thermal shift assay (CETSA), reciprocal co-immunoprecipitation, and siRNA-mediated TLR4 silencing were used to investigate the mechanism. RESULTS: Tet improved metabolic and renal injury indices and alleviated renal histopathological damage in KK-Ay mice. Single-nucleus RNA sequencing revealed bidirectional transcriptional remodeling in proximal tubular cells, including reduced expression of selected inflammation- and injury-associated genes. Structural analyses supported a plausible Tet-TLR4 interaction, while CETSA showed increased TLR4 thermal stability. Reciprocal co-immunoprecipitation supported reduced TLR4-MyD88 association after normalization to the respective immunoprecipitated proteins. Tet reduced TLR4 expression, NF-kappaB p65 activation, and NLRP3-associated inflammatory signaling, with lower mature IL-1beta levels in PA-stimulated HK-2 cells. TLR4 silencing reduced MyD88 abundance, the p-p65/total p65 ratio, and mature IL-1beta and IL-6 levels compared with negative-control siRNA. CONCLUSION: Tet ameliorates experimental diabetic kidney injury, improves metabolic parameters, and attenuates proximal tubular inflammatory responses. The findings support TLR4 as a candidate cellular target and link Tet treatment to reduced TLR4-MyD88 association and attenuation of NF-kappaB/NLRP3-associated inflammatory signaling. CI - Copyright (c) 2026. Published by Elsevier B.V. FAU - Yan, Yan AU - Yan Y AD - Department of Nephrology, Wuhan Hospital of Traditional Chinese Medicine, Wuhan, China; Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China. FAU - Wang, Wenru AU - Wang W AD - Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China. FAU - Chen, Heng AU - Chen H AD - Department of Nephrology, Wuhan Hospital of Traditional Chinese Medicine, Wuhan, China. FAU - Chen, Yao AU - Chen Y AD - Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China. FAU - Liu, Peng AU - Liu P AD - Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China. FAU - Liu, Chenyang AU - Liu C AD - Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China. FAU - Wang, Gang AU - Wang G AD - Department of Nephrology, Wuhan Hospital of Traditional Chinese Medicine, Wuhan, China. Electronic address: ws2311425948@163.com. FAU - Yu, Renhuan AU - Yu R AD - Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China. Electronic address: tezhongeyu@vip.sina.com. FAU - Zhao, Hailing AU - Zhao H AD - Beijing Key Lab for Immune-Mediated Inflammatory Diseases, Department of Nephrology, China-Japan Friendship Hospital, Beijing, China. Electronic address: zhly0167@163.com. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Int Immunopharmacol JT - International immunopharmacology JID - 100965259 SB - IM OTO - NOTNLM OT - Diabetic kidney disease OT - Inflammation OT - Proximal tubular cells OT - TLR4 OT - Tetrandrine COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:12 PHST- 2026/08/19 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:12 [entrez] AID - S1567-5769(26)01460-8 [pii] AID - 10.1016/j.intimp.2026.117612 [doi] PST - aheadofprint SO - Int Immunopharmacol. 2026 Oct 27;191(Pt 1):117612. doi: 10.1016/j.intimp.2026.117612. PMID- 42945914 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-2550 (Electronic) IS - 0304-4017 (Linking) VI - 349 DP - 2026 Oct 21 TI - Targeted selective treatment of gastrointestinal nematodes in lambs: accuracy of the dynamic growth curves. PG - 110959 LID - S0304-4017(26)00278-5 [pii] LID - 10.1016/j.vetpar.2026.110959 [doi] AB - With the objective of validating the use of dynamic growth curves as a targeted selective treatment (TST) criterion, 46 lambs (25 females and 21 males) from the Gralha Azul Experimental Farm of PUCPR were evaluated. The lambs, Texel and Ile de France crossbred, were evaluated from 45 to 150 days of age. Every two weeks, the lambs were weighed and evaluated for packed cell volume (PCV) and fecal egg count (FEC). The need for anthelmintic treatment was indicated by the dynamic growth curves whenever the observed weight was lower than the expected weight. The dynamic growth curves were weighted at each weighing in relation to the previous weight of the lamb. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy were calculated, considering 3 thresholds of FEC, based on the 80th, 85th, and 90th percentile of the cumulative distribution of the FEC as the gold standard. The mean PCV values (31.85% and 32.85%), FEC (1874.99 and 1037.35) and live body weight (27.23 kg and 33.12 kg), respectively for treated and untreated lambs, showed no difference (p > 0.05). For the criterion of FEC >/= 3000, sensitivity was 55%, specificity 71%, PPV 17%, NPV 94% and accuracy 70%. It is concluded that the dynamic curves have a high capacity to identify lambs that do not need treatment (NPV 94%), and additionally that the association with another criterion, such as FEC, in cases where anthelmintic treatment is indicated by the curve, could increase the accuracy of the method. CI - Copyright (c) 2026 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Gogola, Anna Carolina da Silva AU - Gogola ACDS AD - Graduate Program in Animal Science, School of Medicine and Life Sciences, Pontificia Universidade Catolica do Parana (PUCPR), Curitiba, Parana, Brazil. FAU - Santana, Diogenes Adriano Duarte AU - Santana DAD AD - Graduate Program in Animal Science, School of Medicine and Life Sciences, Pontificia Universidade Catolica do Parana (PUCPR), Curitiba, Parana, Brazil. FAU - da Silva, Kassy Gomes AU - da Silva KG AD - Graduate Program in Animal Science, School of Medicine and Life Sciences, Pontificia Universidade Catolica do Parana (PUCPR), Curitiba, Parana, Brazil. FAU - Ratim, Jose Leonardo Sviech AU - Ratim JLS AD - Graduate Program in Animal Science, School of Medicine and Life Sciences, Pontificia Universidade Catolica do Parana (PUCPR), Curitiba, Parana, Brazil. FAU - Weber, Saulo Henrique AU - Weber SH AD - Graduate Program in Animal Science, School of Medicine and Life Sciences, Pontificia Universidade Catolica do Parana (PUCPR), Curitiba, Parana, Brazil. FAU - Sotomaior, Cristina Santos AU - Sotomaior CS AD - Graduate Program in Animal Science, School of Medicine and Life Sciences, Pontificia Universidade Catolica do Parana (PUCPR), Curitiba, Parana, Brazil. Electronic address: cristina.sotomaior@pucpr.br. LA - eng PT - Journal Article DEP - 20261021 PL - Netherlands TA - Vet Parasitol JT - Veterinary parasitology JID - 7602745 SB - IM OTO - NOTNLM OT - Anthelmintic OT - Haemonchus OT - Parasitic infection OT - Sheep OT - Weight gain COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:12 PHST- 2026/05/04 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:12 [entrez] AID - S0304-4017(26)00278-5 [pii] AID - 10.1016/j.vetpar.2026.110959 [doi] PST - aheadofprint SO - Vet Parasitol. 2026 Oct 21;349:110959. doi: 10.1016/j.vetpar.2026.110959. PMID- 42945909 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-4529 (Electronic) IS - 0952-8180 (Linking) VI - 116 DP - 2026 Oct 27 TI - Observational study for perioperative intensive care unit (OPICU): Planned and unplanned ICU admissions, follow-up, discharge, and outcomes. PG - 112323 LID - S0952-8180(26)00202-3 [pii] LID - 10.1016/j.jclinane.2026.112323 [doi] AB - INTRODUCTION: Due to limited resources, identifying high-risk surgical patients who derive clinical benefit from Intensive Care Unit (ICU) admission is essential. Our primary aim was to characterize resource allocation and evaluate the true clinical benefit of ICU admissions using modified Wang criteria. Secondary aim is to identify independent risk factors for ICU admission; validate clinical scoring systems; and compare inter-group outcomes, including Clavien-Dindo complication rates, lengths of stay, and mortality. MATERIALS AND METHODS: This multicenter, prospective, observational study was conducted across 75 ICUs. Adult inpatient surgery cases were categorized into four groups: planned admission (PAG), unplanned admission (UPAG), planned but not admitted (PBNAG), and emergent admission (EAG). Data included preoperative/intraoperative/postoperative(ICU) variables, modified Wang criteria, and mortality rates (ICU, hospital, 30/90-day). RESULTS: Of 55,963 preoperatively evaluated patients, 2764 (4.9%) were planned for ICU admission; 804/2764 patients could not be admitted because of limited ICU beds, remaining of 1960 patients with a planned ICU admission, 411/1960 did not require postoperative ICU care forming the PBNAG (14.8% of 2764). Overall, 1980 patients (3.09% of all cases) were admitted to the ICU (78.2% PAG, 12.5% EAG, 9.1% UPAG). POSSUM scores effectively differentiated PBNAG (lower) and EAG (higher) from PAG. ICU benefit indicators (e.g., vasopressor need, prolonged ventilation >12 h, arrest events) were significantly higher in UPAG and EAG versus PAG (p < 0.001). Emergency surgery (OR 12.4), intraoperative complications (OR 3.1), and ASA >2 (OR 2.7) independently increased UPAG risk, whereas minimally invasive surgery and regional anesthesia reduced ICU admissions. Overall ICU and 90-day mortality rates were 5.9% and 7.5%, respectively, peaking in the EAG group (18.1% and 18.9%). CONCLUSION: Integrating modified Wang criteria, POSSUM scores, intraoperative complications, emergency status, and ASA class >2 is effective in predicting the necessity of ICU admission. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Senturk, Evren AU - Senturk E AD - Koc University School of Medicine Department of Anesthesiology and Intensive Care, Istanbul, Turkiye.. Electronic address: esenturk@ku.edu.tr. FAU - Cakar, Nahit AU - Cakar N AD - Koc University School of Medicine Department of Anesthesiology and Intensive Care, Istanbul, Turkiye.. Electronic address: ncakar@ku.edu.tr. FAU - Ozcan, Perihan Ergin AU - Ozcan PE AD - Istanbul University, Istanbul Faculty of Medicine, Department of Anesthesiology and Intensive Care, Istanbul, Turkiye. Electronic address: pergin@istanbul.edu.tr. FAU - Gucyetmez, Bulent AU - Gucyetmez B AD - Acibadem University, Department of Anesthesiology and Reanimation, Istanbul, Turkiye. Electronic address: bulentgucyetmez@gmail.com. FAU - Dogan, Lerzan AU - Dogan L AD - Acibadem University, Department of Anesthesiology and Reanimation, Istanbul, Turkiye. Electronic address: lredzheb@gmail.com. FAU - Kaynar, Ata Murat AU - Kaynar AM AD - University of Pittsburgh, Department of Critical Care Medicine, Pittsburgh, PA, USA; Departments of Critical Care Medicine and Anesthesiology and Perioperative Medicine, Neurovascular Intensive Care Unit, UPMC Presbyterian, USA. Electronic address: atamuratkaynar@gmail.com. FAU - Ozgultekin, Asu AU - Ozgultekin A AD - University of Health Sciences, Haydarpasa Numune Training and Research Hospital, Istanbul, Turkiye. Electronic address: asuozgultekin@yahoo.com. FAU - Anakli, Ilkay AU - Anakli I AD - Istanbul University, Istanbul Faculty of Medicine, Department of Anesthesiology and Intensive Care, Istanbul, Turkiye. Electronic address: ilkayyenicelioglu@gmail.com. FAU - Polat, Ozlem AU - Polat O AD - Istanbul University, Istanbul Faculty of Medicine, Department of Anesthesiology and Intensive Care, Istanbul, Turkiye. Electronic address: ozlemp@yahoo.com.tr. FAU - Kir, Gulay AU - Kir G AD - Koc University School of Medicine Department of Anesthesiology and Intensive Care, Istanbul, Turkiye.. Electronic address: gkir@kuh.ku.edu.tr. FAU - Cardakozu, Tulay Hosten AU - Cardakozu TH AD - Kocaeli University Faculty of Medicine, Department of Anesthesiology and Intensive Care, Kocaeli, Turkiye. Electronic address: tulayhosten@hotmail.com. FAU - Alparslan, Volkan AU - Alparslan V AD - Kocaeli University Faculty of Medicine, Department of Anesthesiology and Intensive Care, Kocaeli, Turkiye.. Electronic address: volknn@hotmail.com. FAU - Ekinci, Osman AU - Ekinci O AD - University of Health Sciences, Haydarpasa Numune Training and Research Hospital, Istanbul, Turkiye. Electronic address: droekinci@gmail.com. FAU - Karka, Ozlem Ersoy AU - Karka OE AD - Duzce University Faculty of Medicine, Department of Anesthesiology and Intensive Care, Duzce, Turkiye.. Electronic address: drozlemersoy@gmail.com. FAU - Senoglu, Gizem Demir AU - Senoglu GD AD - Duzce University Faculty of Medicine, Department of Anesthesiology and Intensive Care, Duzce, Turkiye.. Electronic address: gizem123demir@hotmail.com. FAU - Buyukcoban, Sibel AU - Buyukcoban S AD - Dokuz Eylul University Faculty of Medicine, Department of Anesthesiology and Intensive Care, Izmir, Turkiye.. Electronic address: sbuyukcoban101@gmail.com. FAU - Gokmen, Ali Necati AU - Gokmen AN AD - Dokuz Eylul University Faculty of Medicine, Department of Anesthesiology and Intensive Care, Izmir, Turkiye.. Electronic address: necati.gokmen@deu.edu.tr. FAU - Tanriverdi, Cagin AU - Tanriverdi C AD - Denizli State Hospital, Department of Anesthesiology and Intensive Care, Denizli, Turkiye. FAU - Erdogmus, Necati Alper AU - Erdogmus NA AD - Denizli State Hospital, Department of Anesthesiology and Intensive Care, Denizli, Turkiye. FAU - Akbulut, Akin AU - Akbulut A AD - Koc University School of Medicine Department of Anesthesiology and Intensive Care, Istanbul, Turkiye.. Electronic address: akakbulut@kuh.ku.edu.tr. FAU - Akca, Ozan AU - Akca O AD - Department of Anesthesiology & Critical Care Medicine (ACCM), Neuro-anesthesia & Neuro-critical care, Johns Hopkins Medicine, United States of America. Electronic address: oakca1@jhu.edu. CN - IREANET network OPICU group FAU - Ulger, Fatma AU - Ulger F AD - Ondokuz Mayis Universitesi Tip Fakultesi, Turkey. FAU - Colak, Ozkul Yilmaz AU - Colak OY AD - Ondokuz Mayis Universitesi Tip Fakultesi, Turkey. FAU - Yildirim, Serap Aktas AU - Yildirim SA AD - Acibadem Altunizade Hastanesi, Turkey. FAU - Sarikaya, Zeynep Tugce AU - Sarikaya ZT AD - Acibadem Altunizade Hastanesi, Turkey. FAU - Guduk, Duygu Emine AU - Guduk DE AD - Acibadem Altunizade Hastanesi, Turkey. FAU - Altun, Dilek AU - Altun D AD - Acibadem International Hastanesi, Turkey. FAU - Kocyigit, Muharrem AU - Kocyigit M AD - Acibadem Maslak Hastanesi, Turkey. FAU - Bayrak, Sunkar Kaya AU - Bayrak SK AD - Acibadem Maslak Hastanesi, Turkey. FAU - Gozubuyuk, Ezgi AU - Gozubuyuk E AD - Acibadem Maslak Hastanesi, Turkey. FAU - Basaran, Burcu AU - Basaran B AD - Acibadem Maslak Hastanesi, Turkey. FAU - Lafci, Ayse AU - Lafci A AD - Ankara Bilkent Sehir Hastanesi 1, Turkey. FAU - Mutlu, Nevzat Mehmet AU - Mutlu NM AD - Ankara Bilkent Sehir Hastanesi 1, Turkey. FAU - Kosovali, Behiye Deniz AU - Kosovali BD AD - Ankara Bilkent Sehir Hastanesi 2, Turkey. FAU - Ozaslan, Nihal Gokbulut AU - Ozaslan NG AD - Ankara Bilkent Sehir Hastanesi 2, Turkey. FAU - Soyal, Ozlem Balkiz AU - Soyal OB AD - Ankara Bilkent Sehir Hastanesi 3, Turkey. FAU - Guven, Alper AU - Guven A AD - Ankara Bilkent Sehir Hastanesi 3, Turkey. FAU - Yavuz, Asiye AU - Yavuz A AD - Ankara Bilkent Sehir Hastanesi 4, Turkey. FAU - Sahap, Mehmet AU - Sahap M AD - Ankara Bilkent Sehir Hastanesi 4, Turkey. FAU - Ozcelik, Menekse AU - Ozcelik M AD - Ankara Universitesi Tip Fakultesi, Turkey. FAU - Bayar, Kemal AU - Bayar K AD - Ankara Universitesi Tip Fakultesi, Turkey. FAU - Dogan, Alper Tunga AU - Dogan AT AD - VKV.Amerikan Hastanesi, Turkey. FAU - Demirdogen, Volkan AU - Demirdogen V AD - VKV.Amerikan Hastanesi, Turkey. FAU - Kavlak, M Emre AU - Kavlak ME AD - Atasehir Memorial Hastanesi, Turkey. FAU - Ugur, Engin AU - Ugur E AD - Atasehir Memorial Hastanesi, Turkey. FAU - Kilinc, Gokhan AU - Kilinc G AD - Balikesir Ataturk Sehir Hastanesi, Turkey. FAU - Yarar, Volkan AU - Yarar V AD - Balikesir Ataturk Sehir Hastanesi, Turkey. FAU - Sagir, Ozlem AU - Sagir O AD - Balikesir Universitesi Tip Fakultesi, Turkey. FAU - Uckun, Serkan AU - Uckun S AD - Balikesir Universitesi Tip Fakultesi, Turkey. FAU - Sahinturk, Helin AU - Sahinturk H AD - Baskent Universitesi Tip Fakultesi, Turkey. FAU - Ayhan, Asude AU - Ayhan A AD - Baskent Universitesi Tip Fakultesi, Turkey. FAU - Akovali, Nukhet AU - Akovali N AD - Baskent Universitesi Tip Fakultesi, Turkey. FAU - Yesiler, Fatma Irem AU - Yesiler FI AD - Baskent Universitesi Tip Fakultesi, Turkey. FAU - Iscimen, Remzi AU - Iscimen R AD - Bursa Uludag Universitesi Tip Fakultesi, Turkey. FAU - Aydin, Leman Gokcenur AU - Aydin LG AD - Bursa Uludag Universitesi Tip Fakultesi, Turkey. FAU - Ozcan, Funda Gumus AU - Ozcan FG AD - Basaksehir Cam ve Sakura Sehir Hastanesi, Turkey. FAU - Gokduman, Huru Ceren AU - Gokduman HC AD - Basaksehir Cam ve Sakura Sehir Hastanesi, Turkey. FAU - Turan, Guldem AU - Turan G AD - Basaksehir Cam ve Sakura Sehir Hastanesi, Turkey. FAU - Baydemir, Abdurrahman Engin AU - Baydemir AE AD - Basaksehir Cam ve Sakura Sehir Hastanesi, Turkey. FAU - Gok, Mehmet Gokhan AU - Gok MG AD - Cukurova Universitesi Tip Fakultesi, Turkey. FAU - Turktan, Mediha AU - Turktan M AD - Cukurova Universitesi Tip Fakultesi, Turkey. FAU - Hatipoglu, Zehra AU - Hatipoglu Z AD - Cukurova Universitesi Tip Fakultesi, Turkey. FAU - Dogan, Omer AU - Dogan O AD - Cukurova Universitesi Tip Fakultesi, Turkey. FAU - Erbatur, Meral Erdal AU - Erbatur ME AD - Diyarbakir Gazi Yasargil EAH, Turkey. FAU - Yanatma, Seher AU - Yanatma S AD - Diyarbakir Gazi Yasargil EAH, Turkey. FAU - Akyol, Fethi AU - Akyol F AD - Erzincan Binali Yildirim Universitesi, Mengucek Gazi EAH, Turkey. FAU - Karaca, Nurcan Kutluer AU - Karaca NK AD - Erzincan Binali Yildirim Universitesi, Mengucek Gazi EAH, Turkey. FAU - Karakoc, Ebru AU - Karakoc E AD - Eskisehir Osmangazi Universitesi Tip Fakultei, Turkey. FAU - Onay, Meryem AU - Onay M AD - Eskisehir Osmangazi Universitesi Tip Fakultei, Turkey. FAU - Kilic, Fatih AU - Kilic F AD - Fatih Sultan Mehmet EAH, Turkey. FAU - Ozdemirkan, Aycan AU - Ozdemirkan A AD - Gazi Universitesi Tip Fakultesi, Turkey. FAU - Pampal, Hasan Kutluk AU - Pampal HK AD - Gazi Universitesi Tip Fakultesi, Turkey. FAU - Bulut, Azime AU - Bulut A AD - Giresun EAH, Turkey. FAU - Bayburt, Fatma Alkan AU - Bayburt FA AD - Giresun EAH, Turkey. FAU - Savas, Asli Melek AU - Savas AM AD - Hacettepe Universitesi Tip Fakultesi, Turkey. FAU - Yildirim, Tugba AU - Yildirim T AD - Hacettepe Universitesi Tip Fakultesi, Turkey. FAU - Sen, Oznur AU - Sen O AD - Haseki EAH, Turkey. FAU - Caliskan, Berna AU - Caliskan B AD - Haseki EAH, Turkey. FAU - Bicakcioglu, Murat AU - Bicakcioglu M AD - Inonu Universitesi Tip Fakultesi, Turkey. FAU - Yagci, Neslihan Altunkaya AU - Yagci NA AD - Inonu Universitesi Tip Fakultesi, Turkey. FAU - Mutlu, Ozgecan Piril Zanbak AU - Mutlu OPZ AD - Istanbul Bahcelievler Devlet Hastanesi, Turkey. FAU - Akyar, Seyma Sayit AU - Akyar SS AD - Istanbul Bahcelievler Devlet Hastanesi, Turkey. FAU - Tunay, Abdurrahman AU - Tunay A AD - Istanbul EAH, Turkey. FAU - Soyturk, Ayse Nur AU - Soyturk AN AD - Istanbul EAH, Turkey. FAU - Atesal, Ozlem AU - Atesal O AD - Istanbul EAH, Turkey. FAU - Kuntman, Atilla AU - Kuntman A AD - Istanbul EAH, Turkey. FAU - Kizilaslan, Deniz AU - Kizilaslan D AD - Istanbul Medipol Universitesi, Turkey. FAU - Yanaral, Tumay Uludag AU - Yanaral TU AD - Istanbul Medipol Universitesi, Turkey. FAU - Akcil, Eren Fatma AU - Akcil EF AD - Istanbul Universitesi-Cerrahpasa, Cerrahpasa Tip Fakultesi, Turkey. FAU - Dilmen, Ozlem Korkmaz AU - Dilmen OK AD - Istanbul Universitesi-Cerrahpasa, Cerrahpasa Tip Fakultesi, Turkey. FAU - Demirel, Ebru Emre AU - Demirel EE AD - Istanbul Universitesi Istanbul Tip Fakultesi, Turkey. FAU - Dincer, Muserref Beril AU - Dincer MB AD - Istanbul Universitesi Istanbul Tip Fakultesi, Turkey. FAU - Ayvat, Pinar AU - Ayvat P AD - Izmir Demokrasi Univeristesi, Turkey. FAU - Asar, Kamil AU - Asar K AD - Izmir Demokrasi Univeristesi, Turkey. FAU - Ozmumcu, Elif Aybike AU - Ozmumcu EA AD - Kanuni Sultan Suleyman EAH, Turkey. FAU - Sahin, Ayca Sultan AU - Sahin AS AD - Kanuni Sultan Suleyman EAH, Turkey. FAU - Cevik, Banu AU - Cevik B AD - Kartal Dr. Lutfi Kirdar Sehir Hastanesi, Turkey. FAU - Bombaci, Elif AU - Bombaci E AD - Kartal Dr. Lutfi Kirdar Sehir Hastanesi, Turkey. FAU - Yilmaz, Ayse AU - Yilmaz A AD - Kastamonu Universitesi Tip Fakultesi, Turkey. FAU - Demir, Ufuk AU - Demir U AD - Kastamonu Universitesi Tip Fakultesi, Turkey. FAU - Taskin, Ozturk AU - Taskin O AD - Kastamonu Universitesi Tip Fakultesi, Turkey. FAU - Soylu, Veysel Garani AU - Soylu VG AD - Kastamonu Universitesi Tip Fakultesi, Turkey. FAU - Kantekin, Cigdem Unal AU - Kantekin CU AD - Kayseri Sehir Hastanesi, Turkey. FAU - Altinkaya, Mine AU - Altinkaya M AD - Kayseri Sehir Hastanesi, Turkey. FAU - Bulut, Akin AU - Bulut A AD - Koc Universitesi Tip Fakultesi, Turkey. FAU - Besir, Ahmet AU - Besir A AD - KTU Tip Fakultesi, Turkey. FAU - Ulusoy, Hulya AU - Ulusoy H AD - KTU Tip Fakultesi, Turkey. FAU - Tugcugil, Ersagun AU - Tugcugil E AD - KTU Tip Fakultesi, Turkey. FAU - Kosucu, Muge AU - Kosucu M AD - KTU Tip Fakultesi, Turkey. FAU - Balci, Canan AU - Balci C AD - Kutahya SBU Evliya Celebi EAH, Turkey. FAU - Kocak, Ozlem AU - Kocak O AD - Kutahya SBU Evliya Celebi EAH, Turkey. FAU - Urkmez, Seval AU - Urkmez S AD - Liv Vadi Hospital, Turkey. FAU - Aygun, Evren AU - Aygun E AD - Liv Vadi Hospital, Turkey. FAU - Tugrul, Mehmet AU - Tugrul M AD - Liv Vadi Hospital, Turkey. FAU - Delen, Leman Acun AU - Delen LA AD - Malatya Turgut Ozal University, Medical Faculty, Turkey. FAU - Aytan, Nilgun Narman AU - Aytan NN AD - Malatya EAH, Turkey. FAU - Gul, Fethi AU - Gul F AD - Marmara Universitesi Tip Fakultesi, Turkey. FAU - Ozlen, Nazan Atalan AU - Ozlen NA AD - Marmara Universitesi Tip Fakultesi, Turkey. FAU - Oktay, Burcin Doruk AU - Oktay BD AD - Marmara Universitesi Pendik EAH, Turkey. FAU - Tekin, Esra AU - Tekin E AD - Marmara Universitesi Pendik EAH, Turkey. FAU - Cakmak, Gul AU - Cakmak G AD - Marmara Universitesi Pendik EAH, Turkey. FAU - Ozden, Mesure Gul Nihan AU - Ozden MGN AD - Medeniyet Universitesi Prof. Dr. Suleyman Yalcin Sehir Hastanesi Goztepe, Turkey. FAU - Goksenoglu, Nezihe Ciftaslan AU - Goksenoglu NC AD - Medeniyet Universitesi Prof. Dr. Suleyman Yalcin Sehir Hastanesi Goztepe, Turkey. FAU - Ozcan, Beysim AU - Ozcan B AD - Memorial Bahcelievler Hastanesi, Turkey. FAU - Demircan, Fatma AU - Demircan F AD - Memorial Bahcelievler Hastanesi, Turkey. FAU - Uluc, Kamuran AU - Uluc K AD - Mus Devlet Hastanesi, Turkey. FAU - Sahin, Murat AU - Sahin M AD - Mus Devlet Hastanesi, Turkey. FAU - Kilinc, Anil AU - Kilinc A AD - Ordu University Medical Faculty, Turkey. FAU - Kerimoglu, Elif AU - Kerimoglu E AD - Ordu EAH, Turkey. FAU - Canoglu, Umut Caner AU - Canoglu UC AD - Ozel Gebze Yuzyil Hastanesi, Turkey. FAU - Kucuk, Rabia Sari AU - Kucuk RS AD - Prof. Dr. Cemil Tascioglu Sehir Hastanesi, Turkey. FAU - Ervetan, Zekeriya AU - Ervetan Z AD - Prof. Dr. Cemil Tascioglu Sehir Hastanesi, Turkey. FAU - Kuzlu, Volkan AU - Kuzlu V AD - Sancaktepe Sehit Prof. Dr. Ilhan Varank EAH, Turkey. FAU - Ozcelik, Zerrin AU - Ozcelik Z AD - Sancaktepe Sehit Prof. Dr. Ilhan Varank EAH, Turkey. FAU - Bakan, Nurten AU - Bakan N AD - Sancaktepe Sehit Prof. Dr. Ilhan Varank EAH, Turkey. FAU - Adiyeke, Esra AU - Adiyeke E AD - Sancaktepe Sehit Prof. Dr. Ilhan Varank EAH, Turkey. FAU - Dilek, Ahmet AU - Dilek A AD - Istinye Universitesi Tip Fakultesi, Turkey. FAU - Er, Mehmet Can AU - Er MC AD - Samsun VM Medikal Park Hastanesi, Turkey. FAU - Karahan, Mahmut Alp AU - Karahan MA AD - SBU, Sanliurfa, Mehmet Akif Inan EAH, Turkey. FAU - Kaya, Ahmet AU - Kaya A AD - SBU, Sanliurfa, Mehmet Akif Inan EAH, Turkey. FAU - Sen, Ahmet AU - Sen A AD - SBU, Trabzon, Tip Fakultesi Kanuni EAH, Turkey. FAU - Akyol, Ahmet AU - Akyol A AD - SBU, Trabzon, Tip Fakultesi Kanuni EAH, Turkey. FAU - Sargin, Mehmet AU - Sargin M AD - Selcuk Universitesi Tip Fakultesi, Turkey. FAU - Celik, Jale Bengi AU - Celik JB AD - Selcuk Universitesi Tip Fakultesi, Turkey. FAU - Bayraktar, Yesim Serife AU - Bayraktar YS AD - Selcuk Universitesi Tip Fakultesi, Turkey. FAU - Ozcan, Mustafa Soner AU - Ozcan MS AD - Suleyman Demirel Universitesi Tip Fakultesi, Turkey. FAU - Karabacak, Pinar AU - Karabacak P AD - Suleyman Demirel Universitesi Tip Fakultesi, Turkey. FAU - Cetin, Nihan AU - Cetin N AD - Taksim Egitim Arastirma Hastanesi, Turkey. FAU - Tekdos, Yasemin AU - Tekdos Y AD - Taksim Egitim Arastirma Hastanesi, Turkey. FAU - Yildirim, Ilker AU - Yildirim I AD - Tekirdag Namik Kemal Universitesi Tip Fakultesi, Turkey. FAU - Arar, Makbule Cavidan AU - Arar MC AD - Tekirdag Namik Kemal Universitesi Tip Fakultesi, Turkey. FAU - Colak, Alkin AU - Colak A AD - Trakya Universitesi Tip Fakultesi, Turkey. FAU - Inal, Mehmet Turan AU - Inal MT AD - Trakya Universitesi Tip Fakultesi, Turkey. FAU - Gultekin, Yucel AU - Gultekin Y AD - Usak Universitesi Tip Fakultesi, Turkey. FAU - Zora, Muhammed Emin AU - Zora ME AD - Usak Universitesi Tip Fakultesi, Turkey. FAU - Tekeli, Arzu Esen AU - Tekeli AE AD - Van Yuzuncu Yil Universitesi Tip Fakultesi, Turkey. FAU - Kurt, Nurettin AU - Kurt N AD - Van Yuzuncu Yil Universitesi Tip Fakultesi, Turkey. FAU - Turan, Raziye AU - Turan R AD - Erzurum Sehir Hastanesi, Turkey. FAU - Ergun, Ahmet AU - Ergun A AD - Erzurum Sehir Hastanesi, Turkey. FAU - Dereli, Necla AU - Dereli N AD - SBU, Ankara, Ataturk Sanatoryum, EAH, Turkey. FAU - Babayigit, Munire AU - Babayigit M AD - SBU, Ankara, Ataturk Sanatoryum, EAH, Turkey. FAU - Abdulkerimov, Vugar AU - Abdulkerimov V AD - Merkezi Klinika, Baku, Turkey. FAU - Gasimov, Emil AU - Gasimov E AD - Yeni Klinika, Baku, Turkey. FAU - Haciyev, Elhan AU - Haciyev E AD - Baku Saglamlik Merkezi Hastanesi, Turkey. FAU - Sungur, Mukadder Orhan AU - Sungur MO AD - Istanbul Universitesi Istanbul Tip Fakultesi, Turkey. FAU - Toraman, Fevzi AU - Toraman F AD - Acibadem Altunizade Hastanesi, Turkey. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Clin Anesth JT - Journal of clinical anesthesia JID - 8812166 SB - IM OTO - NOTNLM OT - Clinical outcomes OT - ICU utilization OT - Intensive care OT - Postoperative care OT - Postoperative period OT - Postoperative unplanned ICU admission. Postoperative emergency admission OT - Triage OT - Triage decision-making COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:12 PHST- 2026/06/05 00:00 [received] PHST- 2026/08/14 00:00 [revised] PHST- 2026/09/10 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:12 [entrez] AID - S0952-8180(26)00202-3 [pii] AID - 10.1016/j.jclinane.2026.112323 [doi] PST - aheadofprint SO - J Clin Anesth. 2026 Oct 27;116:112323. doi: 10.1016/j.jclinane.2026.112323. PMID- 42945908 OWN - NLM STAT- Publisher LR - 20261027 IS - 2531-1387 (Electronic) IS - 2531-1379 (Linking) VI - 49 IP - 1 DP - 2026 Oct 27 TI - Update of the Brazilian Clinical Practice Guideline for primary immune thrombocytopenia: methodological process and main changes for diagnosis and treatment in the Brazilian Unified Health System (SUS). PG - 108564 LID - S2531-1379(26)02311-4 [pii] LID - 10.1016/j.htct.2026.108564 [doi] AB - BACKGROUND: Primary immune thrombocytopenia requires diagnostic exclusion and individualized management. Recent changes in the therapeutic landscape and health technology assessment recommendations of Brazil prompted an update of the national Clinical Practice Guidelines for immune thrombocytopenia in the Unified Health System (SUS). OBJECTIVES: This study aims to describe the methodological process used to update the guidelines for primary immune thrombocytopenia, summarize the primary conceptual, diagnostic, and therapeutic updates, and emphasize the integration of health technology assessment into treatment pathways within the Brazilian Unified Health System. METHODS: The update followed the technical guide of the Brazilian Ministry of Health for guideline development. Recommendations were appraised by the National Committee for Health Technology Incorporation (Conitec) and drafted by a multidisciplinary panel including health technology assessment researchers, clinical experts and policymakers. Systematic reviews, economic evaluations, budget impact analyses, stakeholder input and public consultation drafted the final recommendations. RESULTS: The updated Clinical Practice Guidelines changed the title and conceptual framing to reflect contemporary disease nomenclature and immune‑mediated pathophysiology, reinforced a diagnostic approach based on clinical evaluation and exclusion of alternative causes of thrombocytopenia, and reorganized classification criteria. Rituximab and romiplostim were incorporated for selected adult and pediatric populations with refractory, chronic or corticosteroid‑dependent disease, whereas dapsone was not incorporated. Treatment sequencing was clarified, positioning immunosuppressive therapies before thrombopoietin receptor agonists and specifying age‑related indications. A public consultation refined the wording and interpretation of the therapeutic sequencing, particularly for pediatric care. CONCLUSION: The updated Brazilian Clinical Practice Guidelines for primary immune thrombocytopenia modernizes disease framing, expands treatment options and improves organization of therapeutic decision‑making in the Unified Health System (SUS), illustrating how health technology assessment outputs can be integrated into evidence‑informed national clinical protocols. CI - Copyright (c) 2026 Associacao Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier Espana, S.L.U. All rights reserved. FAU - Gargano, Ludmila Peres AU - Gargano LP AD - Health Technology Assessment Unit, Hospital Alemao Oswaldo Cruz, Sao Paulo, Brazil. FAU - de Paula, Tassiane Cristine Santos AU - de Paula TCS AD - Health Technology Assessment Unit, Hospital Alemao Oswaldo Cruz, Sao Paulo, Brazil. FAU - Oliveira, Layssa Andrade AU - Oliveira LA AD - Health Technology Assessment Unit, Hospital Alemao Oswaldo Cruz, Sao Paulo, Brazil. FAU - Lobo, Clarisse AU - Lobo C AD - Instituto Estadual de Hematologia Arthur de Siqueira Cavalcanti, SES, Rio de Janeiro, Brazil. FAU - Campoy, Francine Doty AU - Campoy FD AD - Santa Catarina Blood Center (HEMOSC), Blumenau, Santa Catarina, Brazil. FAU - Villaca, Paula AU - Villaca P AD - Clinical Hospital of the University of Sao Paulo Medical School (HCFMUSP), Sao Paulo, Brazil. FAU - Nascimento, Ana Clara Kneese AU - Nascimento ACK AD - Faculty of Medical Sciences, Santa Casa de Sao Paulo, Sao Paulo, Brazil. FAU - Junior, Jose Maria Soares AU - Junior JMS AD - Clinical Hospital of the University of Sao Paulo Medical School (HCFMUSP), Sao Paulo, Brazil. FAU - Gomes, Rosangela Maria AU - Gomes RM AD - Brazilian Ministry of Health's (MoH), Brasilia, Brazil. FAU - Maior, Marta da Cunha Lobo Souto AU - Maior MDCLS AD - Brazilian Ministry of Health's (MoH), Brasilia, Brazil. FAU - Lucchetta, Rosa Camila AU - Lucchetta RC AD - Health Technology Assessment Unit, Hospital Alemao Oswaldo Cruz, Sao Paulo, Brazil. Electronic address: rc.lucch@yahoo.com.br. LA - eng PT - Journal Article DEP - 20261027 PL - Brazil TA - Hematol Transfus Cell Ther JT - Hematology, transfusion and cell therapy JID - 101725732 OTO - NOTNLM OT - Health Technology Assessment OT - Idiopathic OT - Practice Guideline OT - Public health practice OT - Purpura OT - Thrombocytopenic COIS- Conflicts of interest The authors declare that they have no conflicts of interest to disclose. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:12 PHST- 2026/07/10 00:00 [received] PHST- 2026/08/17 00:00 [revised] PHST- 2026/09/13 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:12 [entrez] AID - S2531-1379(26)02311-4 [pii] AID - 10.1016/j.htct.2026.108564 [doi] PST - aheadofprint SO - Hematol Transfus Cell Ther. 2026 Oct 27;49(1):108564. doi: 10.1016/j.htct.2026.108564. PMID- 42945903 OWN - NLM STAT- Publisher LR - 20261027 IS - 1980-5322 (Electronic) IS - 1807-5932 (Linking) VI - 81 DP - 2026 Oct 27 TI - Prospective evaluation of indocyanine green fluorescence for anastomotic site selection in laparoscopic colorectal cancer surgery. PG - 101226 LID - S1807-5932(26)00336-4 [pii] LID - 10.1016/j.clinsp.2026.101226 [doi] AB - BACKGROUND: Indocyanine Green (ICG) fluorescence imaging has emerged as a valuable tool for intraoperative assessment of bowel perfusion in colorectal surgery, with the potential to reduce anastomotic leakage. However, standardized protocols for its use remain undefined. This study aimed to evaluate the feasibility and clinical impact of ICG fluorescence imaging in laparoscopic colorectal resections. METHODS: A prospective cohort of 60 patients undergoing laparoscopic sigmoidectomy or low anterior resection for colorectal cancer was analyzed. ICG was administered after high vascular ligation to guide anastomotic site selection. Perfusion was assessed using ICG fluorescence and the marginal arcade test. Primary outcomes were changes in anastomotic site and leakage rates; secondary outcomes included fluorescence time and its association with clinical variables. RESULTS: The mean time to fluorescence appearance was 24.5 seconds (range 8-45), with no significant differences according to sex (p = 0.20), age (p = 0.24), or neoadjuvant therapy (p = 0.38). ICG imaging led to modification of the planned anastomotic site in 6.7% of cases (n = 4), none of which developed leakage. In two cases, discordance between ICG fluorescence and marginal arcade assessment was observed. Overall, anastomotic leakage occurred in 6.7% (n = 4), exclusively in patients who had received neoadjuvant radiotherapy (p = 0.04). No association was found between leakage and anastomotic height, fluorescence time, or marginal arcade findings. No adverse reactions to ICG were reported. CONCLUSION: ICG fluorescence imaging is a safe and feasible adjunct for intraoperative perfusion assessment that supports anastomotic site selection and may contribute to reducing anastomotic leakage. Further studies incorporating quantitative fluorescence metrics are warranted. CI - Copyright (c) 2026. Published by Elsevier Espana, S.L.U. FAU - Imperiale, Antonio Rocco AU - Imperiale AR AD - Instituto do Cancer do Estado de Sao Paulo, Hospital das Clinicas, Faculdade de Medicina, Universidade de Sao Paulo (HCFMUSP), Sao Paulo, SP, Brazil. FAU - Sobrado, Lucas Faraco AU - Sobrado LF AD - Instituto do Cancer do Estado de Sao Paulo, Hospital das Clinicas, Faculdade de Medicina, Universidade de Sao Paulo (HCFMUSP), Sao Paulo, SP, Brazil. Electronic address: lucas.sobrado@hc.fm.usp.br. FAU - Marques, Carlos Frederico Sparapan AU - Marques CFS AD - Instituto do Cancer do Estado de Sao Paulo, Hospital das Clinicas, Faculdade de Medicina, Universidade de Sao Paulo (HCFMUSP), Sao Paulo, SP, Brazil. FAU - Nahas, Caio Sergio Rizkallah AU - Nahas CSR AD - Instituto do Cancer do Estado de Sao Paulo, Hospital das Clinicas, Faculdade de Medicina, Universidade de Sao Paulo (HCFMUSP), Sao Paulo, SP, Brazil. FAU - Cotti, Guilherme Cutait de Castro AU - Cotti GCC AD - Instituto do Cancer do Estado de Sao Paulo, Hospital das Clinicas, Faculdade de Medicina, Universidade de Sao Paulo (HCFMUSP), Sao Paulo, SP, Brazil. FAU - Lopez, Rossana Veronica Mendoza AU - Lopez RVM AD - Instituto do Cancer do Estado de Sao Paulo, Hospital das Clinicas, Faculdade de Medicina, Universidade de Sao Paulo (HCFMUSP), Sao Paulo, SP, Brazil. FAU - Ribeiro-Junior, Ulysses AU - Ribeiro-Junior U AD - Instituto do Cancer do Estado de Sao Paulo, Hospital das Clinicas, Faculdade de Medicina, Universidade de Sao Paulo (HCFMUSP), Sao Paulo, SP, Brazil. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Clinics (Sao Paulo) JT - Clinics (Sao Paulo, Brazil) JID - 101244734 SB - IM OTO - NOTNLM OT - Anastomotic leakage OT - Colorectal surgery OT - Fluorescence imaging OT - Indocyanine green OT - Perfusion assessment COIS- Declaration of competing interest The authors declare no conflicts of interest. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:12 PHST- 2026/04/04 00:00 [received] PHST- 2026/07/27 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:12 [entrez] AID - S1807-5932(26)00336-4 [pii] AID - 10.1016/j.clinsp.2026.101226 [doi] PST - aheadofprint SO - Clinics (Sao Paulo). 2026 Oct 27;81:101226. doi: 10.1016/j.clinsp.2026.101226. PMID- 42945902 OWN - NLM STAT- Publisher LR - 20261027 IS - 1878-1292 (Electronic) IS - 1878-1241 (Linking) VI - 63 DP - 2026 Oct 23 TI - Improved rehabilitation outcomes in patients following unilateral dual-channel endoscopic spinal surgery through comprehensive personalised education combined with the CICARE communication model: A prospective randomised controlled trial. PG - 101333 LID - S1878-1241(26)00078-X [pii] LID - 10.1016/j.ijotn.2026.101333 [doi] AB - BACKGROUND: Unilateral biportal endoscopic spine surgery (UBE) is a minimally invasive technique associated with reduced surgical trauma. However, postoperative rehabilitation presents challenges including pain management and functional recovery, with significant variation in individual rehabilitation needs. Traditional standardised patient education approaches struggle to meet these diverse requirements. The CICARE (Connect, Introduce, Communicate, Ask, Respond, and Exit) communication model provides a structured approach to patient-centred communication, while comprehensive personalised health education tailors information and guidance to patients' individual needs throughout the care process. This study aimed to evaluate the effects of combining these two approaches on postoperative rehabilitation outcomes in patients undergoing unilateral biportal endoscopic spine surgery (UBE). METHODS: This study employed a randomised controlled design, enrolling 120 patients undergoing UBE surgery at a tertiary hospital in Henan Province between June 2024 and March 2025. Participants were allocated to an intervention group and a control group using a random number table. The control group received routine care, while the observation group underwent a comprehensive personalised education programme combined with CICARE communication intervention. The General Self-Efficacy Scale (GSES), Self-Rating Anxiety Scale (SAS), Self-Rating Depression Scale (SDS), Short-Form McGill Pain Questionnaire (SF-MPQ), and Oswestry Disability Index (ODI) scores were compared between the two groups preoperatively and at 1 and 12 weeks postoperatively. Postoperative time to ambulation, length of hospital stay, and patient satisfaction with health education were also compared between the groups. RESULTS: Preoperative data between the two groups showed no statistically significant differences(p > 0.05). Following intervention, the intervention group demonstrated significantly superior GSES, SAS, SDS, SF-MPQ, and ODI scores compared to the control group (p < 0.05). Postoperative time to ambulation and hospital stay were significantly shorter than in the control group (p < 0.05) while patient satisfaction was higher than in the control group(p = 0.04). CONCLUSION: The implementation of full-process personalised health education nursing integrated with the CICARE communication model enhances patients' general self-efficacy, reduces the occurrence of anxiety, depression and pain, promotes patients' functional recovery, shortens time to ambulation and hospital stay, and improves patient satisfaction. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Li, Aoxue AU - Li A AD - Department of Mini-invasive Spinal Surgery, The Third People's Hospital of Henan Province, China. FAU - Ma, Hongna AU - Ma H AD - Department of Mini-invasive Spinal Surgery, The Third People's Hospital of Henan Province, China. FAU - Tian, Xiufeng AU - Tian X AD - Department of Mini-invasive Spinal Surgery, The Third People's Hospital of Henan Province, China. FAU - Jia, Xiaowei AU - Jia X AD - Department of Mini-invasive Spinal Surgery, The Third People's Hospital of Henan Province, China. FAU - Wang, Xiaoling AU - Wang X AD - Department of Mini-invasive Spinal Surgery, The Third People's Hospital of Henan Province, China. FAU - Qiao, Haiyang AU - Qiao H AD - Department of Mini-invasive Spinal Surgery, The Third People's Hospital of Henan Province, China. FAU - Shen, Mingkui AU - Shen M AD - Department of Mini-invasive Spinal Surgery, The Third People's Hospital of Henan Province, China; Henan Engineering Research Center of Precision Diagnosis and Treatment of Intervertebral Disc Disease, China. FAU - Yang, Hejun AU - Yang H AD - Department of Mini-invasive Spinal Surgery, The Third People's Hospital of Henan Province, China. FAU - Cheng, Shina AU - Cheng S AD - Department of Mini-invasive Spinal Surgery, The Third People's Hospital of Henan Province, China. Electronic address: csnhehe@qq.com. LA - eng PT - Journal Article DEP - 20261023 PL - England TA - Int J Orthop Trauma Nurs JT - International journal of orthopaedic and trauma nursing JID - 101528681 SB - IM OTO - NOTNLM OT - CICARE communication model OT - Personalised nursing care OT - Postoperative rehabilitation OT - Unilateral biportal endoscopic COIS- Conflict of interest The authors declare that they have no competing interests. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:11 PHST- 2026/08/17 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:11 [entrez] AID - S1878-1241(26)00078-X [pii] AID - 10.1016/j.ijotn.2026.101333 [doi] PST - aheadofprint SO - Int J Orthop Trauma Nurs. 2026 Oct 23;63:101333. doi: 10.1016/j.ijotn.2026.101333. PMID- 42945901 OWN - NLM STAT- Publisher LR - 20261027 IS - 1532-8708 (Electronic) IS - 0093-7754 (Linking) VI - 53 IP - 6 DP - 2026 Oct 9 TI - Pretreatment platelet count exhibits a U-shaped association with outcomes after immune checkpoint blockade with a mid-range stratum for dual-checkpoint immunotherapy. PG - 152567 LID - S0093-7754(26)00112-0 [pii] LID - 10.1016/j.seminoncol.2026.152567 [doi] AB - BACKGROUND: Platelets contribute to tumor progression and immune regulation, but the relationship between pretreatment platelet count and immune checkpoint blockade (ICB) outcomes across the clinical range remains unclear. METHODS: This study conducted a retrospective multicohort study of patients treated with ICB. In a Memorial Sloan Kettering pan-cancer cohort (n = 1,479), platelet count was modeled using penalized cubic splines and clinically defined strata, with 150-249 x 10(9)/L as the reference. Cox models adjusted for age, sex, prior chemotherapy, ICB class, tumor stage, cancer type, and tumor mutational burden. Findings were evaluated in a validation cohort (n = 717) and in non-small cell lung cancer (n = 666) and melanoma (n = 377) analyses. An exploratory pooled analysis (n = 2,196) compared mono-ICB with dual PD-1/PD-L1 plus CTLA-4 blockade across platelet strata. RESULTS: Platelet count showed a U-shaped association with overall survival. Relative to 150-249 x 10(9)/L, adjusted mortality hazards were higher at 49-124 x 10(9)/L (hazard ratio [HR], 1.83; p = 0.0001), 300-399 x 10(9)/L (HR, 1.56; p < 0.0001), and 400-977 x 10(9)/L (HR, 1.93; p < 0.0001). High counts were also associated with shorter progression-free survival (HR, 1.27 for 300-399 x 10(9)/L and 1.32 for 400-977 x 10(9)/L; both p = 0.003), whereas associations at low counts were weaker. Similar patterns were observed in the validation and cancer-specific cohorts. Dual ICB was associated with longer median overall survival than mono-ICB only in the 150-300 x 10(9)/L stratum (2.34 vs 1.42 years; p < 0.0001). CONCLUSIONS: Pretreatment platelet count is an inexpensive biomarker with a nonlinear association with ICB outcomes. Both low and high counts identify increased mortality risk, supporting flexible modeling rather than a single thrombocytosis threshold. The platelet-defined signal for dual ICB is hypothesis-generating and requires prospective validation before treatment selection. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Zheng, Ming AU - Zheng M AD - Human Disease Continuum Project Consortium, Beijing, China; New City of Chang'an, No. 28 Dacheng Road, Fengtai District, Beijing, 100141, China. Electronic address: mmzheng@fmmu.edu.cn. LA - eng PT - Letter DEP - 20261009 PL - United States TA - Semin Oncol JT - Seminars in oncology JID - 0420432 SB - IM OTO - NOTNLM OT - Immune checkpoint blockade OT - Immunotherapy OT - Overall survival OT - Pan-cancer analysis OT - Platelet count OT - Progression-free survival COIS- Declaration of competing interest The author declares that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:11 PHST- 2026/06/14 00:00 [received] PHST- 2026/08/23 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:11 [entrez] AID - S0093-7754(26)00112-0 [pii] AID - 10.1016/j.seminoncol.2026.152567 [doi] PST - aheadofprint SO - Semin Oncol. 2026 Oct 9;53(6):152567. doi: 10.1016/j.seminoncol.2026.152567. PMID- 42945891 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-7072 (Electronic) IS - 0308-8146 (Linking) VI - 532 IP - Pt 4 DP - 2026 Oct 26 TI - Dy-MOF-based electrochemiluminescence probe regulated by ACQ inhibition and Ti(2)AlC conductivity for targeted detection of sulfadiazine. PG - 151623 LID - S0308-8146(26)03783-0 [pii] LID - 10.1016/j.foodchem.2026.151623 [doi] AB - Sulfadiazine (SDZ) residues in animal-derived foods posed a potential risk to human health. We constructed a molecularly imprinted electrochemiluminescence sensor (MIECLS) for selective detection of SDZ based on the framework confinement effect of a dysprosium-based metal-organic framework (Dy-MOF) luminophore and the electron transfer acceleration effect of Ti(2)AlC. A Dy-MOF with eight-coordinate Dy(3+) centers was synthesized under microwave assisted coordination between Dy(3+) and 9,10-di(p-carboxyphenyl)anthracene (DPA). The rigid Dy-MOF framework suppressed pi-pi stacking by spatially isolating DPA ligands and reduced nonradiative relaxation by restricting intramolecular motion of DPA, thereby mitigating aggregation-caused quenching (ACQ). Ti(2)AlC suppressed Dy-MOF aggregation via electrostatic interactions and facilitated electron transfer through its good conductivity, thereby enhancing ECL intensity. By employing molecularly imprinted polymer (MIP) as specific recognition elements, the developed MIECLS exhibited linear range of 1-500,000 nmol/L with a limit of detection of 0.552 nmol/L, providing a promising strategy for rapid food safety monitoring. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Han, Dewang AU - Han D AD - State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China. FAU - Yang, Kaiwen AU - Yang K AD - State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China. FAU - Qin, Yumeng AU - Qin Y AD - State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China. FAU - Cai, Lin AU - Cai L AD - State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China. FAU - Hao, Wen AU - Hao W AD - State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China. FAU - Guo, Mengmeng AU - Guo M AD - State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China. FAU - Fang, Guozhen AU - Fang G AD - State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China. Electronic address: fangguozhen@tust.edu.cn. FAU - Wang, Shuo AU - Wang S AD - Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin, 300071, China. Electronic address: wangshuo@nankai.edu.cn. LA - eng PT - Journal Article DEP - 20261026 PL - England TA - Food Chem JT - Food chemistry JID - 7702639 SB - IM OTO - NOTNLM OT - ACQ OT - Dy-MOF OT - Electrochemiluminescence OT - Molecularly imprinted polymer OT - Sulfadiazine OT - Ti(2)AlC COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:11 PHST- 2026/03/16 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:11 [entrez] AID - S0308-8146(26)03783-0 [pii] AID - 10.1016/j.foodchem.2026.151623 [doi] PST - aheadofprint SO - Food Chem. 2026 Oct 26;532(Pt 4):151623. doi: 10.1016/j.foodchem.2026.151623. PMID- 42945884 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-7072 (Electronic) IS - 0308-8146 (Linking) VI - 532 IP - Pt 4 DP - 2026 Oct 24 TI - Surface-engineered magnetic shellac microcapsules as recyclable Pickering interfacial biocatalysts for enzymatic synthesis of partial Acylglycerols. PG - 151770 LID - S0308-8146(26)03930-0 [pii] LID - 10.1016/j.foodchem.2026.151770 [doi] AB - Enzymatic synthesis of partial acylglycerols from glycerol and free fatty acids is often limited by poor interfacial mass transfer and low product selectivity. Herein, surface-engineered lipase-loaded magnetic microcapsules were fabricated using natural shellac as recyclable Pickering interfacial biocatalysts. Fe(3)O(4) nanoparticles imparted magnetic responsiveness, while the hydrophobic/hydrophilic fumed silica ratio tuned surface wettability and interfacial behavior. Results show that optimal surface wettability yielded uniform Pickering emulsion droplets. The optimized sample M5 exhibited 3.26-fold higher apparent activity than Novozym 435. In glycerol-fatty acid esterification, M5 achieved comparable fatty acid conversion to Novozym 435 while significantly suppressing triacylglycerol formation and enhancing partial acylglycerol selectivity, reaching 86.09% total yield. The catalyst retained high activity after six magnetic recycling cycles. This study establishes a wettability-controllable Pickering biocatalysis platform, offering a promising green strategy for selective enzymatic production of functional lipids rich in partial acylglycerols for food and nutraceutical applications. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Jiang, Cong AU - Jiang C AD - State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, People's Republic of China. FAU - Meng, Zong AU - Meng Z AD - State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, People's Republic of China. Electronic address: mengzong@jiangnan.edu.cn. LA - eng PT - Journal Article DEP - 20261024 PL - England TA - Food Chem JT - Food chemistry JID - 7702639 SB - IM OTO - NOTNLM OT - Immobilized lipase OT - Magnetic recovery OT - Partial acylglycerols OT - Pickering interfacial biocatalysis OT - Shellac microcapsules OT - Wettability COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:11 PHST- 2026/05/12 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:11 [entrez] AID - S0308-8146(26)03930-0 [pii] AID - 10.1016/j.foodchem.2026.151770 [doi] PST - aheadofprint SO - Food Chem. 2026 Oct 24;532(Pt 4):151770. doi: 10.1016/j.foodchem.2026.151770. PMID- 42945881 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-7072 (Electronic) IS - 0308-8146 (Linking) VI - 532 IP - Pt 4 DP - 2026 Oct 27 TI - Mechanisms of flavor quality enhancement in mechanically harvested flake-shaped raw tea materials via light shaking: An integrated analysis of intelligent sensory evaluation, metabolomics, and enzyme activities. PG - 151792 LID - S0308-8146(26)03952-X [pii] LID - 10.1016/j.foodchem.2026.151792 [doi] AB - Non-selective mechanical harvesting yields flake-shaped raw tea materials with uneven maturity and poor integrity, thereby constraining the flavor quality of finished tea. This study applied light shaking to improve flavor and used multi-omics to uncover the underlying regulatory mechanisms. The results showed that shaking decreased the ratio of tea polyphenols to total free amino acids by 13.91% and catechin content by 12.73%, while increasing total volatiles by 84.86%. Bitterness, astringency, and green aroma were significantly reduced, whereas umami, floral, fruity, and sweet aromas were markedly enhanced. A total of 60 non-volatile and 90 volatile metabolites were identified, with 21 key compounds driving the flavor profile shift. Mechanistically, shaking, as an abiotic stress, increased phenylalanine and tryptophan levels by 64.07% and 23.16%, respectively, and enhanced the activities of 13 key flavor-related metabolic enzymes. These findings provide a theoretical basis for improving the quality of flake-shaped fresh tea leaves. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Zhang, Jixin AU - Zhang J AD - State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China; School of Tea Science, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China. FAU - Zhang, Yuxuan AU - Zhang Y AD - State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China; School of Tea Science, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China. FAU - Yang, Luyao AU - Yang L AD - State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China; School of Tea Science, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China. FAU - Chen, Zhenbin AU - Chen Z AD - State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China; School of Tea Science, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China. FAU - Ye, Zhibin AU - Ye Z AD - State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China; School of Tea Science, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China. FAU - Jiang, Yanqun AU - Jiang Y AD - State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China; School of Tea Science, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China. FAU - Li, Tiehan AU - Li T AD - State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China; School of Tea Science, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China. FAU - Gan, Ning AU - Gan N AD - State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China; School of Tea Science, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China. FAU - Hu, Shaode AU - Hu S AD - State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China; School of Tea Science, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China. FAU - Ning, Jingming AU - Ning J AD - State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China; School of Tea Science, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China. Electronic address: ningjm1998009@163.com. FAU - Zhang, Zhengzhu AU - Zhang Z AD - State Key Laboratory of Tea Plant Germplasm Innovation and Resource Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China; School of Tea Science, Anhui Agricultural University, 130 Changjiang West Road, Hefei, 230036, China. Electronic address: zzz@ahau.edu.cn. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Food Chem JT - Food chemistry JID - 7702639 SB - IM OTO - NOTNLM OT - Enzyme activity OT - Flavor compounds OT - Green tea OT - Intelligent sensory evaluation OT - Shaking COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:11 PHST- 2026/02/15 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:11 [entrez] AID - S0308-8146(26)03952-X [pii] AID - 10.1016/j.foodchem.2026.151792 [doi] PST - aheadofprint SO - Food Chem. 2026 Oct 27;532(Pt 4):151792. doi: 10.1016/j.foodchem.2026.151792. PMID- 42945876 OWN - NLM STAT- Publisher LR - 20261027 IS - 1768-3254 (Electronic) IS - 0223-5234 (Linking) VI - 321 IP - Pt 3 DP - 2026 Oct 21 TI - Novel amidino-substituted phenylene-bis(benzazoles): Design, synthesis, biological evaluation and DNA binding properties. PG - 119456 LID - S0223-5234(26)00901-3 [pii] LID - 10.1016/j.ejmech.2026.119456 [doi] AB - A series of symmetrical amidino-substituted phenylene-bis(benzazoles) incorporating benzoxazole, benzothiazole and benzimidazole scaffolds was designed to explore the influence of the heteroaromatic core, substitution pattern and amidine type on antiproliferative and antitrypanosomal activity. Distinct structure-activity relationships were observed for the two biological activity profiles. Antiproliferative activity strongly depended on both the heteroaromatic scaffold and the nature of the amidine moiety. Among the investigated series, bis(benzoxazoles) showed the highest potency, with C-6 imidazolinyl derivatives 7da and 7 db displaying submicromolar inhibitory activity across a panel of human cancer cell lines (EC(50) = 0.06-2.5 muM). Evaluation in PBMCs indicated cancer cell selectivity, while cell cycle analysis of the most active derivatives revealed G2/M phase accumulation. In contrast, antitrypanosomal activity against bloodstream-form Trypanosoma brucei followed a different SAR profile, with several derivatives showing pronounced potency and favourable selectivity indices. In particular, the benzoxazole derivative 7 ab bearing an unsubstituted amidine exhibited excellent potency and selectivity (EC(50) = 0.044 muM, SI = 920), while the benzothiazole derivative 3aa also demonstrated strong activity (EC(50) = 0.09 muM, SI = 325). In the benzimidazole series, the imidazolinyl derivative 9ba showed a highly favourable profile (EC(50) = 0.05 muM, SI = 675). Biophysical studies revealed that 9ba exhibits high affinity toward AT-rich nucleic acid structures, including triplex DNA and DNA:RNA hybrids. Integration of biological and biophysical data indicates that nucleic acid recognition may contribute to the biological activity of this series. Overall, the distinct SAR profiles identified 7 ab as the most promising antitrypanosomal lead for further optimization towards the development of potent and selective antitrypanosomal agents. CI - Copyright (c) 2026 Elsevier Masson SAS. All rights reserved. FAU - Pticek, Lucija AU - Pticek L AD - University of Zagreb Faculty of Textile Technology, Department of Applied Chemistry, Prilaz Baruna Filipovica 28a, Zagreb, 10000, Croatia. FAU - Bec, Anja AU - Bec A AD - University of Zagreb Faculty of Textile Technology, Department of Applied Chemistry, Prilaz Baruna Filipovica 28a, Zagreb, 10000, Croatia. FAU - Zonjic, Iva AU - Zonjic I AD - Ruder Boskovic Institute, Division of Organic Chemistry and Biochemistry, Bijenicka Cesta 54, Zagreb, 10000, Croatia. FAU - Tumir, Lidija-Marija AU - Tumir LM AD - Ruder Boskovic Institute, Division of Organic Chemistry and Biochemistry, Bijenicka Cesta 54, Zagreb, 10000, Croatia. FAU - Stojkovic, Marijana Radic AU - Stojkovic MR AD - Ruder Boskovic Institute, Division of Organic Chemistry and Biochemistry, Bijenicka Cesta 54, Zagreb, 10000, Croatia. Electronic address: mradic@irb.hr. FAU - Guncevic, Kristina AU - Guncevic K AD - Ruder Boskovic Institute, Division of Organic Chemistry and Biochemistry, Bijenicka Cesta 54, Zagreb, 10000, Croatia. FAU - Persoons, Leentje AU - Persoons L AD - KU Leuven, Rega Institute for Medical Research, Department of Microbiology, Immunology and Transplantation, Molecular Genetics and Therapeutics in Virology and Oncology Research Group, Herestraat 49, Box 1048, Leuven, 3000, Belgium. FAU - Daelemans, Dirk AU - Daelemans D AD - KU Leuven, Rega Institute for Medical Research, Department of Microbiology, Immunology and Transplantation, Molecular Genetics and Therapeutics in Virology and Oncology Research Group, Herestraat 49, Box 1048, Leuven, 3000, Belgium. FAU - Taylor, Martin C AU - Taylor MC AD - London School of Hygiene and Tropical Medicine, Department of Infection Biology, London, WC1E 7HT, UK. FAU - Kelly, John M AU - Kelly JM AD - London School of Hygiene and Tropical Medicine, Department of Infection Biology, London, WC1E 7HT, UK. FAU - Racane, Livio AU - Racane L AD - University of Zagreb Faculty of Textile Technology, Department of Applied Chemistry, Prilaz Baruna Filipovica 28a, Zagreb, 10000, Croatia. Electronic address: livio.racane@ttf.unizg.hr. LA - eng PT - Journal Article DEP - 20261021 PL - France TA - Eur J Med Chem JT - European journal of medicinal chemistry JID - 0420510 SB - IM OTO - NOTNLM OT - Amidines OT - Antiproliferative activity OT - Antitrypanosomal activity OT - Polynucleotide binding OT - bis(benzazoles) COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:11 PHST- 2026/07/10 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:11 [entrez] AID - S0223-5234(26)00901-3 [pii] AID - 10.1016/j.ejmech.2026.119456 [doi] PST - aheadofprint SO - Eur J Med Chem. 2026 Oct 21;321(Pt 3):119456. doi: 10.1016/j.ejmech.2026.119456. PMID- 42945875 OWN - NLM STAT- Publisher LR - 20261027 IS - 1768-3254 (Electronic) IS - 0223-5234 (Linking) VI - 321 IP - Pt 3 DP - 2026 Oct 24 TI - Cancer-selective cytotoxicity of a semi-synthetic derivative of oleuropein via proteostasis network impairment. PG - 119466 LID - S0223-5234(26)00911-6 [pii] LID - 10.1016/j.ejmech.2026.119466 [doi] AB - Cancer is a leading cause of death worldwide, driving continued research for innovative therapies with enhanced specificity and efficacy. Natural products have long been recognised as a valuable source of anticancer agents due to their structural diversity and unique biological activities. Building on the anticancer potential of oleuropein, a major olive tree (Olea europaea) metabolite, we evaluated a newly synthesised semi-synthetic analogue, GS32, for its cytotoxic activity in vitro. Cytotoxicity assays revealed that GS32 exhibited broad-spectrum activity against various cancer cell lines, while also displaying differential toxicity between normal and cancerous cells. Through transcriptomic analysis, we discovered that GS32 differentially disrupts key cellular pathways involved in stress response. Importantly, targeted phosphoproteomic profiling uncovered context-dependent modulation of key signalling pathways, including the AKT/mTOR and NF-kappaB axes, as well as stress-response regulators. Further experiments confirmed that GS32 disrupts the proteostasis network, leading to excessive proteotoxic stress, mainly in malignant cells. This study provides new mechanistic insight into the cellular effects of GS32 and identifies the compound as a candidate that merits further preclinical investigation. CI - Copyright (c) 2026 The Authors. Published by Elsevier Masson SAS.. All rights reserved. FAU - Gianniou, Despoina D AU - Gianniou DD AD - Department of Cell Biology and Biophysics, Faculty of Biology, National and Kapodistrian University of Athens, Athens, 15784, Greece. FAU - Kontos, Christos K AU - Kontos CK AD - Department of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, Athens, Greece. FAU - Gkogkou, Eirini AU - Gkogkou E AD - Department of Cell Biology and Biophysics, Faculty of Biology, National and Kapodistrian University of Athens, Athens, 15784, Greece. FAU - Sarikaki, Georgia AU - Sarikaki G AD - Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece. FAU - Karousi, Paraskevi AU - Karousi P AD - Department of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, Athens, Greece. FAU - Skaltsounis, Alexios-Leandros AU - Skaltsounis AL AD - Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece. FAU - Scorilas, Andreas AU - Scorilas A AD - Department of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, Athens, Greece. FAU - Kostakis, Ioannis K AU - Kostakis IK AD - Department of Pharmaceutical Chemistry, Faculty of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece. Electronic address: ikkostakis@pharm.uoa.gr. FAU - Trougakos, Ioannis P AU - Trougakos IP AD - Department of Cell Biology and Biophysics, Faculty of Biology, National and Kapodistrian University of Athens, Athens, 15784, Greece. Electronic address: itrougakos@biol.uoa.gr. LA - eng PT - Journal Article DEP - 20261024 PL - France TA - Eur J Med Chem JT - European journal of medicinal chemistry JID - 0420510 SB - IM OTO - NOTNLM OT - Cancer OT - Natural products OT - Oleuropein OT - Proteostasis network OT - RNA sequencing (RNA-seq) COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:11 PHST- 2026/04/09 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:11 [entrez] AID - S0223-5234(26)00911-6 [pii] AID - 10.1016/j.ejmech.2026.119466 [doi] PST - aheadofprint SO - Eur J Med Chem. 2026 Oct 24;321(Pt 3):119466. doi: 10.1016/j.ejmech.2026.119466. PMID- 42945856 OWN - NLM STAT- Publisher LR - 20261027 IS - 1096-0341 (Electronic) IS - 0042-6822 (Linking) VI - 625 DP - 2026 Oct 26 TI - Reciprocal regulation between NDP52 and PRRSV: a bidirectional host-virus interplay. PG - 111108 LID - S0042-6822(26)00324-7 [pii] LID - 10.1016/j.virol.2026.111108 [doi] AB - Porcine reproductive and respiratory syndrome virus (PRRSV) is a major swine pathogen responsible for substantial economic losses worldwide. The selective autophagy receptor NDP52 plays a critical role in antiviral defense, yet its function during PRRSV infection remains poorly understood. In this study, we show that NDP52 restricts PRRSV replication: overexpression reduced viral titers and N protein levels, while knockdown enhanced replication. NDP52 specifically reduced Nsp12 protein levels, which was partially reversed by the autophagy inhibitor 3-methyladenine (3-MA). Conversely, PRRSV infection decreased NDP52 protein levels at late stages without affecting its mRNA levels. Screening of viral proteins identified Nsp1alpha, Nsp10, and GP3 as being associated with reduced NDP52 levels; this reduction was blocked by 3-MA. Together, these findings suggest a bidirectional host-virus interplay, in which NDP52 restricts viral replication by reducing Nsp12, while the virus counteracts this restriction via Nsp1alpha, Nsp10, and GP3.The precise mechanisms, including the role of autophagy, require further investigation. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Yi, Heyou AU - Yi H AD - Key Laboratory of Animal Pathogen Infection and Immunology of Fujian Province, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Joint Laboratory of Animal Pathogen Prevention and Control of Fujian-Nepal, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address: heyouyi@fafu.edu.cn. FAU - Wang, Shaojun AU - Wang S AD - Guangdong Provincial Key Laboratory of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510462, China. FAU - Guo, Guijie AU - Guo G AD - Key Laboratory of Animal Pathogen Infection and Immunology of Fujian Province, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Key Laboratory of Fujian-Taiwan Animal Pathogen Biology, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Joint Laboratory of Animal Pathogen Prevention and Control of Fujian-Nepal, College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address: guoguijie@fafu.edu.cn. LA - eng PT - Journal Article DEP - 20261026 PL - United States TA - Virology JT - Virology JID - 0110674 SB - IM OTO - NOTNLM OT - Host-virus interaction OT - NDP52 OT - PRRSV OT - Protein reduction OT - Viral replication COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:10 PHST- 2026/08/17 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:10 [entrez] AID - S0042-6822(26)00324-7 [pii] AID - 10.1016/j.virol.2026.111108 [doi] PST - aheadofprint SO - Virology. 2026 Oct 26;625:111108. doi: 10.1016/j.virol.2026.111108. PMID- 42945854 OWN - NLM STAT- Publisher LR - 20261027 IS - 1876-035X (Electronic) IS - 1876-0341 (Linking) VI - 19 IP - 12 DP - 2026 Oct 23 TI - Candidozyma auris in hospital setting: Molecular characterization of clinical and environmental strains. PG - 103404 LID - S1876-0341(26)00276-5 [pii] LID - 10.1016/j.jiph.2026.103404 [doi] AB - BACKGROUND: Candidozyma auris is a yeast that has a strong ability to adapt, can cause serious invasive infections and contaminate various surfaces. METHODS: After cases of C. auris have occurred in patients hospitalized in a healthcare facility of Apulia region, Italy, clinical and environmental surveillance was implemented. The aim of this study is to perform genotypic and phenotypic characterization between clinical and environmental isolations. In August and September 2024, in three hospital wards, axillary-inguinal swabs were performed on hospitalized patients; environmental monitoring was carried out simultaneously to detect C. auris in air and surfaces. RESULTS: C. auris strain was isolated from the blood culture and peritoneal fluid of a patient, and identified using mass spectrometry. Swabs were performed on surfaces in the patient's room using sterile swabs, analyzed by molecular method. Active and passive sampling of air was conducted. Out of the 194 surface swabs, C. auris DNA was detected in 50 samples. Passive air sampling identified C. auris in 33.3% of plates in Intensive Care patient areas. In the Infectious Diseases Unit, a sample tested positive. Active air sampling didn't detect C. auris. 66 patients were screened, all of which resulted negative for C. auris. Swabs collected from two patients were positive. All isolates were susceptible to amphotericin B, anidulafungin and resistant to fluconazole. Amino acid substitutions were identified in the ERG11 and TAC1B genes. CONCLUSIONS: Viable C. auris was detected in a small number of environmental samples and was genetically closely related to isolates from the corresponding patients. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Caggiano, Giuseppina AU - Caggiano G AD - Interdisciplinary Department of Medicine, Hygiene Section, University of Bari Aldo Moro, Bari, Italy. FAU - Triggiano, Francesco AU - Triggiano F AD - Interdisciplinary Department of Medicine, Hygiene Section, University of Bari Aldo Moro, Bari, Italy. Electronic address: francesco.triggiano@uniba.it. FAU - Fioriti, Simona AU - Fioriti S AD - Department of Biomedical Sciences and Public Health, Universita Politecnica delle Marche, Ancona, Italy. FAU - D'Achille, Gloria AU - D'Achille G AD - Department of Biomedical Sciences and Public Health, Universita Politecnica delle Marche, Ancona, Italy. FAU - Rochira, Nicoletta AU - Rochira N AD - Department of Precision and Regenerative Medicine and Ionian Area (DiMePre-J), University of Bari Aldo Moro, Bari, Italy. FAU - Diella, Giusy AU - Diella G AD - Interdisciplinary Department of Medicine, Hygiene Section, University of Bari Aldo Moro, Bari, Italy. FAU - Spagnuolo, Valentina AU - Spagnuolo V AD - Interdisciplinary Department of Medicine, Hygiene Section, University of Bari Aldo Moro, Bari, Italy. FAU - Lopuzzo, Marco AU - Lopuzzo M AD - Interdisciplinary Department of Medicine, Hygiene Section, University of Bari Aldo Moro, Bari, Italy. FAU - Dalfino, Lidia AU - Dalfino L AD - Department of Precision and Regenerative Medicine and Ionian Area(DiMePRe-J), Section of Anesthesiology and Intensive Care Medicine, University of Bari Aldo Moro, Italy. FAU - Stufano, Monica AU - Stufano M AD - Department of Precision and Regenerative Medicine and Ionian Area(DiMePRe-J), Section of Anesthesiology and Intensive Care Medicine, University of Bari Aldo Moro, Italy. FAU - Sartini, Marina AU - Sartini M AD - Department of Health Sciences, University of Genoa, Genoa, Italy; Operating Unit Hospital Hygiene, Galliera Hospital, Genoa, 16128, Italy. FAU - Cristina, Maria Luisa AU - Cristina ML AD - Department of Health Sciences, University of Genoa, Genoa, Italy; Operating Unit Hospital Hygiene, Galliera Hospital, Genoa, 16128, Italy. FAU - Grasso, Salvatore AU - Grasso S AD - Department of Precision and Regenerative Medicine and Ionian Area(DiMePRe-J), Section of Anesthesiology and Intensive Care Medicine, University of Bari Aldo Moro, Italy. FAU - Di Lorenzo, Antonio AU - Di Lorenzo A AD - Interdisciplinary Department of Medicine, Hygiene Section, University of Bari Aldo Moro, Bari, Italy. FAU - Tafuri, Silvio AU - Tafuri S AD - Interdisciplinary Department of Medicine, Hygiene Section, University of Bari Aldo Moro, Bari, Italy. FAU - Barchiesi, Francesco AU - Barchiesi F AD - Department of Biomedical Sciences and Public Health, Universita Politecnica delle Marche, Ancona, Italy; Infectious Diseases Unit, Azienda Sanitaria Territoriale 1, Pesaro, Pesaro-Urbino, Italy. FAU - Morroni, Gianluca AU - Morroni G AD - Department of Biomedical Sciences and Public Health, Universita Politecnica delle Marche, Ancona, Italy; S.O.D. Medicina di Laboratorio, Azienda Ospedaliero-Universitaria delle Marche, Ancona, Italy. LA - eng PT - Journal Article DEP - 20261023 PL - England TA - J Infect Public Health JT - Journal of infection and public health JID - 101487384 SB - IM OTO - NOTNLM OT - Antifungal susceptibility OT - Biofilm OT - Candidemia OT - Candidozyma auris OT - ICU COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:10 PHST- 2026/08/17 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:10 [entrez] AID - S1876-0341(26)00276-5 [pii] AID - 10.1016/j.jiph.2026.103404 [doi] PST - aheadofprint SO - J Infect Public Health. 2026 Oct 23;19(12):103404. doi: 10.1016/j.jiph.2026.103404. PMID- 42945850 OWN - NLM STAT- Publisher LR - 20261027 IS - 1090-2147 (Electronic) IS - 0278-2626 (Linking) VI - 199 DP - 2026 Oct 27 TI - Theta-frequency sensory entrainment promotes episodic memory consolidation. PG - 106489 LID - S0278-2626(26)00097-7 [pii] LID - 10.1016/j.bandc.2026.106489 [doi] AB - Neural oscillations in the theta frequency range have been implicated in episodic memory processes. Previous work using EEG neurofeedback and non-invasive brain stimulation has demonstrated that enhancing theta activity immediately after encoding can improve long-term memory retention. The present study examined whether audio-visual entrainment combined with cranio-electro stimulation (AVE + CES), a noninvasive and accessible method, may similarly support early stages of consolidation. Sixty-nine undergraduate participants were assigned to one of three oscillatory sensory entrainment conditions: Theta 3 Hz and Theta 5.5 Hz (to examine frequency-specific theta effects), or Beta 16 Hz (as a control condition). Participants completed three study-test cycles of an episodic free-recall task, followed by 20 min of AVE + CES during which they were exposed to rhythmically flashing lights, pulsing tones, and mild earlobe electrical stimulation. Memory performance was assessed immediately after entrainment, after 24 h, and after one week. Baseline-corrected retention scores were computed relative to pre-entrainment performance. No group differences were observed prior to entrainment and baseline-corrected retention did not differ between groups immediately following entrainment. However, both theta groups exhibited significantly reduced forgetting relative to the beta group after 24 h and one week, with no differences between the two theta frequencies. These findings suggest that theta-frequency sensory entrainment broadly enhances episodic memory consolidation, highlighting AVE + CES as a promising and accessible approach for promoting mnemonic retention. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Shtoots, Limor AU - Shtoots L AD - Dina Recanati School of Medicine, Reichman University, Herzliya, 4610101, Israel. Electronic address: slimor@runi.ac.il. FAU - Haim Israel, Barr AU - Haim Israel B AD - Baruch Ivcher School of Psychology, Reichman University, Herzliya, 4610101, Israel. FAU - Partouche, Roni AU - Partouche R AD - Baruch Ivcher School of Psychology, Reichman University, Herzliya, 4610101, Israel. FAU - Levy, Daniel A AU - Levy DA AD - Baruch Ivcher School of Psychology, Reichman University, Herzliya, 4610101, Israel; Department of Psychology, Palo Alto University, Palo Alto, California, 94304, USA. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Brain Cogn JT - Brain and cognition JID - 8218014 SB - IM OTO - NOTNLM OT - Audio-visual entrainment OT - Cranial electrostimulation OT - Episodic memory OT - Memory consolidation OT - Neural oscillations OT - Non-invasive neuromodulation OT - Theta oscillation COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:10 PHST- 2026/08/14 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:10 [entrez] AID - S0278-2626(26)00097-7 [pii] AID - 10.1016/j.bandc.2026.106489 [doi] PST - aheadofprint SO - Brain Cogn. 2026 Oct 27;199:106489. doi: 10.1016/j.bandc.2026.106489. PMID- 42945836 OWN - NLM STAT- Publisher LR - 20261027 IS - 1525-3171 (Electronic) IS - 0032-5791 (Linking) VI - 105 IP - 12 DP - 2026 Oct 23 TI - A multi-species synbiotic improves growth performance in broiler chickens under conventional and enteric-challenge conditions: a meta-analysis. PG - 108031 LID - S0032-5791(26)01665-2 [pii] LID - 10.1016/j.psj.2026.108031 [doi] AB - This study evaluated the efficacy of a multi-species synbiotic (SYN) comprising Bifidobacterium animalis, Enterococcus faecium, Ligilactobacillus salivarius, Limosilactobacillus reuteri, Pediococcus acidilactici, and fructo-oligosaccharides on broiler growth performance and footpad dermatitis across multiple trials conducted under conventional conditions and under experimentally induced enteric challenges. Challenge models included Eimeria spp. (with or without Clostridium perfringens) and Salmonella Enteritidis exposure, alongside comparisons against antibiotic growth promoters (AGP) or coccidiostats in selected studies. Fifteen independent studies contributed to the growth performance meta-analyses, of which three also contributed to the footpad dermatitis analysis. The analysis included trials from 2011 to 2023 with well-defined designs, duration >/=40 days, and reported sample sizes and standard deviations, categorizing them into conventional studies, enteric challenges (Eimeria spp. +/- C. perfringens or Salmonella Enteritidis), and comparisons with AGP or coccidiostats. Meta-analysis results, corrected by Hedges' g, showed standardized mean differences (SMD) indicating medium effect sizes for body weight (BW, SMD = 0.74, P < 0.001), average daily gain (ADG, SMD = 0.59, P = 0.003), and feed conversion ratio (FCR, SMD = -0.56, P < 0.001) in broilers under conventional conditions. In challenged birds under experimental conditions, SYN exhibited medium to large effects on BW (SMD = 0.90, P = 0.007), ADG (SMD = 1.26, P = 0.002) and FCR (SMD = -0.56, P = 0.006), significantly outperforming untreated controls. Moreover, growth performance did not differ significantly between SYN and AGP or coccidiostat treatments in the available trials. SYN supplementation was associated with lower odds of footpad dermatitis (OR = 0.52; 95% CI = [0.28, 0.98]; P = 0.046), although heterogeneity was substantial. These results demonstrate that SYN supplementation enhances broiler growth performance, while the pooled footpad dermatitis analysis indicated lower odds of lesions. These findings suggest that SYN may have potential within strategies aimed at reducing reliance on antimicrobial growth promoters. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Pasquali, Guilherme A M AU - Pasquali GAM AD - dsm-firmenich, Animal Nutrition and Health, Kaiseraugst, Switzerland. Electronic address: guilherme.pasquali@dsm-firmenich.com. FAU - Kesselring, Jutta C AU - Kesselring JC AD - dsm-firmenich, Animal Nutrition and Health R&D Center, Tulln, Austria. FAU - Evgeniou, Michail AU - Evgeniou M AD - dsm-firmenich, Animal Nutrition and Health R&D Center, Tulln, Austria. FAU - Papadopoulou, Ermioni AU - Papadopoulou E AD - dsm-firmenich, Animal Nutrition and Health R&D Center, Tulln, Austria. FAU - Mohnl, Michaela AU - Mohnl M AD - dsm-firmenich, Animal Nutrition and Health R&D Center, Tulln, Austria. LA - eng PT - Journal Article DEP - 20261023 PL - England TA - Poult Sci JT - Poultry science JID - 0401150 SB - IM OTO - NOTNLM OT - Feed additive OT - Footpad dermatitis OT - Gut microbiota OT - Prebiotic OT - Probiotic COIS- Disclosures All authors are employed by dsm-firmenich Animal Nutrition and Health. The authors declare no additional competing interests. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:09 PHST- 2026/07/14 00:00 [received] PHST- 2026/10/21 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:09 [entrez] AID - S0032-5791(26)01665-2 [pii] AID - 10.1016/j.psj.2026.108031 [doi] PST - aheadofprint SO - Poult Sci. 2026 Oct 23;105(12):108031. doi: 10.1016/j.psj.2026.108031. PMID- 42945832 OWN - NLM STAT- Publisher LR - 20261027 IS - 1095-6859 (Electronic) IS - 0090-8258 (Linking) VI - 214 DP - 2026 Oct 27 TI - Heterogeneity of folate receptor alpha expression and associations with molecular biomarker in ovarian high-grade serous carcinoma: A multicenter Chinese cohort study. PG - 182-189 LID - S0090-8258(26)02133-5 [pii] LID - 10.1016/j.ygyno.2026.09.008 [doi] AB - BACKGROUND: Folate receptor alpha (FRalpha) is an established therapeutic target in ovarian cancer, but information on its expression distribution, staining heterogeneity, and molecular context in Chinese patients remains limited. We evaluated these features in ovarian high grade serous carcinoma (HGSC). METHODS: This retrospective multicenter study included 1002 patients with resected HGSC from 14 Chinese hospitals. FRalpha expression was assessed using the VENTANA FOLR1 (FOLR1-2.1) RxDx Assay based on the percentage of viable tumor cells with moderate or strong (2+/3+) membranous staining. Intensity specific percentages were available for 992 tumors. Associations with clinicopathologic features and BRCA1/2, HRD, TMB, MSI, MMR, HER2, and PD-L1 were exploratory. Temporal concordance was evaluated in 53 paired primary and recurrent tumors. RESULTS: High FRalpha expression, defined as 2+/3+ staining in >/=75% of viable tumor cells, was observed in 435 of 1002 tumors (43.41%). Among 992 tumors with intensity data, 972 (98.0%) contained at least two staining categories, and 924 (93.1%) contained both negative or weak and moderate or strong tumor cell populations. No significant associations were detected with age, FIGO stage, tumor size, or the biomarkers evaluated, although analyses were limited and underpowered. Paired tumors showed 71.7% categorical agreement (Cohen's kappa = 0.44) and moderate correlation of FRalpha percentages (Spearman rho = 0.59). CONCLUSIONS: FRalpha expression showed substantial variation between tumors and frequent intensity admixture within specimens. Biomarker associations remain inconclusive because of limited testing. Moderate primary and recurrent concordance supports archival testing, while discordance suggests repeat testing at recurrence may be appropriate in selected patients. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Li, Feifei AU - Li F AD - Department of Pathology, Seventh Medical Center, PLA General Hospital, Beijing 100700, China. FAU - Li, Danhui AU - Li D AD - Department of Pathology, RenJi Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 200127, China. FAU - Xi, Yun AU - Xi Y AD - Department of Pathology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310006, China. FAU - Wang, Wei AU - Wang W AD - Department of Pathology, West China Second Hospital of Sichuan University, Chengdu, Sichuan 610041, China. FAU - Yang, Yang AU - Yang Y AD - Department of Pathology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510080, China. FAU - Bian, Lijuan AU - Bian L AD - Department of Pathology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510000, China. FAU - Li, Shenglei AU - Li S AD - Department of Pathology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450002, China; Henan Key Laboratory of Tumor Pathology, Zhengzhou, Henan 450002, China; Department of Pathology, School of Basic Medicine, Zhengzhou University, Zhengzhou, Henan 450002, China. FAU - Yue, Junqiu AU - Yue J AD - Department of Pathology, Hubei Cancer Hospital, Wuhan, Hubei 430079, China. FAU - Cao, Wenfeng AU - Cao W AD - Department of Pathology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China. FAU - Zhang, Jing AU - Zhang J AD - Department of Pathology, Xijing Hospital, The Fourth Military Medical University, Xi'an, Shanxi 710032, China. FAU - Song, Yan AU - Song Y AD - Department of Pathology, Cancer Hospital Chinese Academy of Medical Sciences, Beijing 100021, China. FAU - Lin, Xuyong AU - Lin X AD - Department of Pathology, The First Hospital of China Medical University, Shenyang, Liaoning 110001, China. FAU - Liang, Zhiyong AU - Liang Z AD - Department of Pathology, Peking Union Medical College Hospital, Beijing 100730, China. FAU - Shi, Xiaohua AU - Shi X AD - Department of Pathology, Peking Union Medical College Hospital, Beijing 100730, China. FAU - Bi, Rui AU - Bi R AD - Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai 200032, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China. FAU - Jiang, He AU - Jiang H AD - Department of Pathology, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China. FAU - Cao, Dengfeng AU - Cao D AD - Department of Pathology, RenJi Hospital, Shanghai JiaoTong University School of Medicine, Shanghai 200127, China. Electronic address: caodengfeng@renji.com. FAU - Liu, Aijun AU - Liu A AD - Department of Pathology, Seventh Medical Center, PLA General Hospital, Beijing 100700, China. Electronic address: liuaj@301hospital.com.cn. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Gynecol Oncol JT - Gynecologic oncology JID - 0365304 SB - IM OTO - NOTNLM OT - Folate receptor alpha OT - Immunohistochemistry OT - Ovarian high-grade serous carcinoma OT - Recurrent ovarian cancer OT - Staining heterogeneity COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:09 PHST- 2026/06/20 00:00 [received] PHST- 2026/09/04 00:00 [revised] PHST- 2026/09/10 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:09 [entrez] AID - S0090-8258(26)02133-5 [pii] AID - 10.1016/j.ygyno.2026.09.008 [doi] PST - aheadofprint SO - Gynecol Oncol. 2026 Oct 27;214:182-189. doi: 10.1016/j.ygyno.2026.09.008. PMID- 42945831 OWN - NLM STAT- Publisher LR - 20261027 IS - 0975-9476 (Print) IS - 0975-9476 (Linking) VI - 17 IP - 6 DP - 2026 Oct 27 TI - Molecular mechanisms and therapeutic potential of Rasayana in cellular protection and healthy aging. PG - 101378 LID - S0975-9476(26)00062-8 [pii] LID - 10.1016/j.jaim.2026.101378 [doi] AB - Aging is a complex biological process characterized by oxidative stress, cellular senescence, and metabolic dysregulation. Ayurveda, the ancient Indian system of medicine, emphasizes longevity and rejuvenation through Rasayana therapy, a specialized branch that enhances vitality, delays aging, and prevents age-related diseases. This review explores the mechanistic basis of Ayurvedic antioxidants, including Ashwagandha (Withania somnifera), Amalaki (Emblica officinalis), Guduchi (Tinospora cordifolia), Shatavari (Asparagus racemosus), Haritaki (Terminalia chebula), Brahmi (Bacopa monnieri), and Curcumin (Curcuma longa), in promoting longevity. These herbs modulate key aging pathways, including oxidative stress reduction, mitochondrial protection, immune modulation, neuroprotection, telomere maintenance, and endocrine balance. Scientific evidence highlights their potential in mitigating chronic inflammation, enhancing cognitive function, and improving metabolic homeostasis, thereby increasing lifespan and healthspan. Systematic electronic database search via PubMed, Scopus, and Google Scholar with the use of the keywords "Ayurveda," "Rasayana," "antioxidants," "longevity," and "anti-aging herbs" was conducted. English-language peer-reviewed papers were selected on the basis of relevance, mechanistic content, and availability of experimental or clinical evidence. The evidence was collated to identify patterns of therapy and mechanisms in common. Despite promising outcomes, the clinical translation of Ayurvedic antioxidants faces challenges such as limited standardized trials, bioavailability concerns, and regulatory hurdles. Future directions include integrating Ayurvedic principles with modern medicine through personalized therapeutics, nutraceutical innovations, and synergistic formulations. Standardized clinical trials and molecular validation are essential for their acceptance in global healthcare. This review provides a comprehensive analysis of Ayurvedic antioxidants as potent natural interventions for healthy aging. CI - Copyright (c) 2026 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Gulhane, Rutuja AU - Gulhane R AD - Division of Neurotherapeutics, Smt. Kishoritai Bhoyar College of Pharmacy, Dist. Nagpur, Kamptee, Maharashtra, India. FAU - Nistane, Neha AU - Nistane N AD - Division of Neurotherapeutics, Smt. Kishoritai Bhoyar College of Pharmacy, Dist. Nagpur, Kamptee, Maharashtra, India. FAU - Qutub, Mohammad AU - Qutub M AD - Division of Neurotherapeutics, Smt. Kishoritai Bhoyar College of Pharmacy, Dist. Nagpur, Kamptee, Maharashtra, India. FAU - Tatode, Amol AU - Tatode A AD - Division of Neurotherapeutics, Smt. Kishoritai Bhoyar College of Pharmacy, Dist. Nagpur, Kamptee, Maharashtra, India. FAU - Umekar, Milind AU - Umekar M AD - Division of Neurotherapeutics, Smt. Kishoritai Bhoyar College of Pharmacy, Dist. Nagpur, Kamptee, Maharashtra, India. FAU - Taksande, Brijesh AU - Taksande B AD - Department of Pharmacology, Smt. Kishoritai Bhoyar College of Pharmacy, Dist. Nagpur, Kamptee, Maharashtra, India. FAU - Taksande, Jayshree AU - Taksande J AD - Division of Neurotherapeutics, Smt. Kishoritai Bhoyar College of Pharmacy, Dist. Nagpur, Kamptee, Maharashtra, India. Electronic address: jayabtaksande@gmail.com. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - United States TA - J Ayurveda Integr Med JT - Journal of Ayurveda and integrative medicine JID - 101551404 OTO - NOTNLM OT - Anti-aging OT - Ayurveda OT - Longevity OT - Mitochondrial protection OT - Oxidative stress OT - Rasayana therapy COIS- Declaration of competing interest The authors declare that there are no conflicts of interest regarding the publication of this manuscript. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:09 PHST- 2025/05/22 00:00 [received] PHST- 2026/05/31 00:00 [revised] PHST- 2026/06/01 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:09 [entrez] AID - S0975-9476(26)00062-8 [pii] AID - 10.1016/j.jaim.2026.101378 [doi] PST - aheadofprint SO - J Ayurveda Integr Med. 2026 Oct 27;17(6):101378. doi: 10.1016/j.jaim.2026.101378. PMID- 42945829 OWN - NLM STAT- Publisher LR - 20261027 IS - 1872-8952 (Electronic) IS - 1388-2457 (Linking) VI - 193 DP - 2026 Oct 27 TI - Erratum to "Selective Attention Deficits in Mild Traumatic Brain Injury using Steady-State Visual Evoked Potentials; Neural Correlates of Attention" [Clin. Neurophysiol. 192 (2026), 2112387]. PG - 2112442 LID - S1388-2457(26)00942-9 [pii] LID - 10.1016/j.clinph.2026.2112442 [doi] FAU - Norouzpour, Amir AU - Norouzpour A AD - Spencer Center for Vision Research, Byers Eye Institute, Stanford University, United States of America. Electronic address: amirn@stanford.edu. FAU - Haensel, Jennifer X AU - Haensel JX AD - Spencer Center for Vision Research, Byers Eye Institute, Stanford University, United States of America. FAU - Lee, Minyoung AU - Lee M AD - Department of Psychology, Stanford University, United States of America. FAU - Hu, Amber AU - Hu A AD - Spencer Center for Vision Research, Byers Eye Institute, Stanford University, United States of America. FAU - Vandiver, Tyler J AU - Vandiver TJ AD - Spencer Center for Vision Research, Byers Eye Institute, Stanford University, United States of America. FAU - Slinger, Kristin E AU - Slinger KE AD - Spencer Center for Vision Research, Byers Eye Institute, Stanford University, United States of America. FAU - Ameyaw Baah, Christabel A AU - Ameyaw Baah CA AD - Spencer Center for Vision Research, Byers Eye Institute, Stanford University, United States of America. FAU - Leonen, Joellen AU - Leonen J AD - Spencer Center for Vision Research, Byers Eye Institute, Stanford University, United States of America. FAU - Lew, Caitlyn Y AU - Lew CY AD - Spencer Center for Vision Research, Byers Eye Institute, Stanford University, United States of America. FAU - Srinivasan, Gayathri AU - Srinivasan G AD - Spencer Center for Vision Research, Byers Eye Institute, Stanford University, United States of America. FAU - Lumba-Brown, Angela AU - Lumba-Brown A AD - Department of Emergency Medicine, School of Medicine, Stanford University, United States of America. FAU - Gardner, Justin L AU - Gardner JL AD - Department of Psychology, Stanford University, United States of America; Wu Tsai Neurosciences Institute, Stanford University, United States of America. FAU - Raghuram, Aparna AU - Raghuram A AD - Boston Children's Hospital, Harvard Medical School, Boston, United States of America. FAU - Norcia, Anthony M AU - Norcia AM AD - Department of Psychology, Stanford University, United States of America; Wu Tsai Neurosciences Institute, Stanford University, United States of America. FAU - Roberts, Tawna L AU - Roberts TL AD - Spencer Center for Vision Research, Byers Eye Institute, Stanford University, United States of America. LA - eng PT - Published Erratum DEP - 20261027 PL - Netherlands TA - Clin Neurophysiol JT - Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology JID - 100883319 SB - IM EFR - Clin Neurophysiol. 2026 Dec;192:2112387. doi: 10.1016/j.clinph.2026.2112387. PMID: 42731193 EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:09 PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:09 [entrez] AID - S1388-2457(26)00942-9 [pii] AID - 10.1016/j.clinph.2026.2112442 [doi] PST - aheadofprint SO - Clin Neurophysiol. 2026 Oct 27;193:2112442. doi: 10.1016/j.clinph.2026.2112442. PMID- 42945811 OWN - NLM STAT- Publisher LR - 20261027 IS - 1532-8171 (Electronic) IS - 0735-6757 (Linking) VI - 111 DP - 2026 Oct 21 TI - Subcutaneous insulin for mild-to-moderate diabetic ketoacidosis: a scoping review and implementation framework. PG - 38-46 LID - S0735-6757(26)00548-6 [pii] LID - 10.1016/j.ajem.2026.10.023 [doi] AB - PURPOSE: Diabetic ketoacidosis (DKA) is a common emergency department (ED) presentation associated with high morbidity, mortality, and healthcare utilization. While intravenous (IV) insulin remains the most common treatment, emerging data suggest subcutaneous (SUBQ) insulin may be a feasible alternative for patients with mild-to-moderate DKA. This scoping review summarizes current evidence of SUBQ insulin use across a broad range of clinical and operational outcomes in DKA and provides an implementation framework for SUBQ insulin for mild-to-moderate DKA in the ED. METHODS: We conducted a scoping review of PubMed from inception through February 2026 for adult studies evaluating SUBQ insulin for DKA. Two investigators independently screened studies, with disagreements resolved by consensus. Data were charted and synthesized across clinical, operational, and implementation outcomes. RESULTS: A total of 117 articles were identified and 13 included. Available evidence suggests SUBQ insulin may achieve similar time to DKA resolution, compared with IV insulin in select patients with uncomplicated DKA. SUBQ insulin offers potential operational benefits, including reduced intensive care unit (ICU) utilization, shorter ED length of stay, and improved resource optimization. Patient selection criteria, dosing and monitoring strategies, and implementation approaches are summarized. Limitations in the literature include small sample sizes, single-center designs, heterogeneous protocols, and the exclusion of several higher-risk patient populations. CONCLUSIONS: Evidence suggests SUBQ insulin pathways achieve similar clinical outcomes and may reduce ICU utilization and improve operational efficiency in select patients with mild-to-moderate DKA. Because most evidence originates from small, single-center cohorts, future studies should focus on larger, more generalizable populations. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Royce, Steven AU - Royce S AD - Anne Burnett Marion School of Medicine at Texas Christian University, Fort Worth, TX, USA. Electronic address: steven.royce@tcu.edu. FAU - Murali, Shyam AU - Murali S AD - Division of Acute Care Surgery, Department of Surgery, Baylor University Medical Center, Dallas, TX, USA. Electronic address: shyam.murali@bswhealth.org. FAU - Claude, Enoch AU - Claude E AD - Department of Pharmacy, WakeMed Health and Hospitals, Raleigh, NC, USA. Electronic address: eclaude@wakemed.org. FAU - Htet, Natalie N AU - Htet NN AD - Department of Emergency Medicine, Division of Critical Care Medicine, Stanford University, Palo Alto, CA, USA. Electronic address: nhtet@stanford.edu. FAU - Jafari, Daniel AU - Jafari D AD - Department of Emergency Medicine, Northwell Health Feinstein Institutes for Medical Research, Manhasset, NY, USA; Northwell, New Hyde Park, NY, USA. Electronic address: djafari@northwell.edu. FAU - Huddleston, Boglarka S AU - Huddleston BS AD - Lane Medical Library, Stanford University, Palo Alto, CA, USA. Electronic address: b.huddleston@stanford.edu. FAU - Acquisto, Nicole M AU - Acquisto NM AD - Department of Pharmacy, University of Rochester Medicine, Rochester, NY, USA; Department of Emergency Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY, USA. Electronic address: nicole_acquisto@urmc.rochester.edu. FAU - Tran, Quincy K AU - Tran QK AD - Research Associate Program in Emergency Medicine & Critical Care, Department of Emergency Medicine, University of Maryland School of Medicine, Baltimore, MD, USA; Department of Emergency Medicine, University of Maryland School of Medicine, Baltimore, MD, USA; Program In Trauma, The R Adam Cowley Shock Trauma Center, University of Maryland School of Medicine, Baltimore, MD, USA. Electronic address: qtran@som.umaryland.edu. FAU - Walker, Jennifer A AU - Walker JA AD - Anne Burnett Marion School of Medicine at Texas Christian University, Fort Worth, TX, USA; Department of Emergency Medicine, Baylor Scott and White All Saints Medical Center, Fort Worth, TX, USA. Electronic address: jennifer.walker@bswhealth.org. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - United States TA - Am J Emerg Med JT - The American journal of emergency medicine JID - 8309942 SB - IM OTO - NOTNLM OT - Diabetes mellitus OT - Diabetic ketoacidosis OT - Emergency service OT - Hospital OT - Humans OT - Insulins COIS- Declaration of competing interest SR, SM, EC, NNH, BSH, NMA, QKT, JAW have no relevant disclosures. DJ has received a research grant for CARES AI from the Laerdal foundation and is a physician investor in Novelna Inc. These relationships are unrelated to the current work. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:09 PHST- 2026/07/23 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:09 [entrez] AID - S0735-6757(26)00548-6 [pii] AID - 10.1016/j.ajem.2026.10.023 [doi] PST - aheadofprint SO - Am J Emerg Med. 2026 Oct 21;111:38-46. doi: 10.1016/j.ajem.2026.10.023. PMID- 42945809 OWN - NLM STAT- Publisher LR - 20261027 IS - 1532-8171 (Electronic) IS - 0735-6757 (Linking) VI - 111 DP - 2026 Oct 19 TI - Magnesium sulfate therapy for adult emergency department patients with acute asthma: A narrative review. PG - 23-31 LID - S0735-6757(26)00536-X [pii] LID - 10.1016/j.ajem.2026.10.011 [doi] AB - BACKGROUND: Magnesium sulfate (MgSO4) has been used for decades as an adjunctive bronchodilator in acute asthma exacerbations refractory to first-line therapy, but its role in adult emergency department (ED) care remains debated. OBJECTIVE: To review the evidence for intravenous (IV) and nebulized MgSO4 as adjunctive therapy in adults presenting to the ED with acute asthma, to clarify how the pooled analyses and the largest individual trial relate to one another, and to compare this evidence with current guideline recommendations. METHODS: This is a structured narrative review. Records were identified from a PubMed search using a single search phrase (19 records), supplemented by targeted Cochrane Library and guideline-body searches performed in August 2026 (14 records) and by backward citation searching. Screening and data extraction were performed by a single reviewer. After removing duplicates and one record excluded for population mismatch, 29 records were assessed for eligibility. Ten provided extractable magnesium-specific efficacy or safety data, 5 primary randomized controlled trials (RCTs) and 5 systematic reviews or meta-analyses, which are presented separately because the reviews pool overlapping trials. The remaining 19 records were used only as background. Eighteen additional eligible RCTs included in these reviews were not identified by our search; these are listed, and their contribution is represented only through the pooled estimates of the reviews that include them. RESULTS: In pooled analysis, IV MgSO4 was associated with a modest reduction in hospital admission (Cochrane 2014, 11 trials including the 3 Mg trial: OR 0.75, 95% CI 0.60-0.92; number needed to treat approximately 14) and some short-term improvement in lung function. The largest individual trial (3 Mg, n = 1109), which contributes to that pooled estimate, found a similar but non-significant reduction in admission with IV MgSO4 (OR 0.73, 95% CI 0.51-1.04), no benefit from nebulized MgSO4, and more adverse events with active treatment than with placebo (15.6% vs 10.1%; OR 1.68, 95% CI 1.11-2.52), most of which were minor. A larger lung-function benefit at the lowest baseline FEV1 was supported by one trial's prespecified interaction analysis, but a larger reduction in admission in the most severely obstructed patients rests on a small subgroup and was not confirmed by a test for subgroup differences across trials. A 2023 adult-specific meta-analysis found no statistically significant pooled lung-function benefit by either route. Commonly reported adverse effects were flushing, fatigue, nausea, headache, and transient hypotension. CONCLUSION: IV MgSO4 may provide a modest benefit in selected adults with severe asthma exacerbations that do not respond to initial therapy. A greater benefit in the most severely obstructed patients is plausible but has not been established. Nebulized MgSO4 has no convincing evidence of benefit in adults. Major guidelines (GINA 2026, BTS/SIGN 158 [2019], and NAEPP EPR-3 [2007]) support IV MgSO4 as a second-line adjunct for severe or life-threatening exacerbations unresponsive to initial therapy, and GINA has not recommended nebulized MgSO4 since its 2025 update. Clinicians using IV MgSO4 in severe refractory asthma should expect a modest and uncertain benefit and an excess of minor, but rarely serious, adverse events. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Peacock, W Frank AU - Peacock WF AD - Emergency Medicine, Baylor College of Medicine, Houston, TX, United States of America. Electronic address: Frankpeacock@gmail.com. FAU - Singer, Adam J AU - Singer AJ AD - Department of Emergency Medicine, Renaissance School of Medicine, Stony Brook University, Stony Brook, NY, United States of America. LA - eng PT - Journal Article PT - Review DEP - 20261019 PL - United States TA - Am J Emerg Med JT - The American journal of emergency medicine JID - 8309942 SB - IM OTO - NOTNLM OT - Acute asthma exacerbation OT - Magnesium sulphate COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:09 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:09 [entrez] AID - S0735-6757(26)00536-X [pii] AID - 10.1016/j.ajem.2026.10.011 [doi] PST - aheadofprint SO - Am J Emerg Med. 2026 Oct 19;111:23-31. doi: 10.1016/j.ajem.2026.10.011. PMID- 42945808 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-5495 (Electronic) IS - 1467-8039 (Linking) VI - 95 DP - 2026 Oct 27 TI - Embryonic and early postembryonic development of the freshwater isopod crustacean Asellus aquaticus - staging based on morphology. PG - 101574 LID - S1467-8039(26)00068-X [pii] LID - 10.1016/j.asd.2026.101574 [doi] AB - The developmental biology of arthropods has advanced rapidly in recent decades, largely through the application of novel experimental techniques to a limited number of established model organisms. Extending comparative embryology beyond these traditional models is essential for a comprehensive understanding of arthropod evolution. Among crustaceans, the freshwater isopod Asellus aquaticus Linnaeus (1758) represents a promising emerging evo-devo model. This widespread European species inhabits diverse freshwater environments, including groundwater, resulting in pronounced phenotypic variation, most apparent in cave-adapted populations. It is easy to culture in the laboratory, reproduces year-round, and produces numerous accessible embryos with relatively fast development. Here, we present the most comprehensive developmental staging system for A. aquaticus to date. Using light microscopy, fluorescence microscopy with DAPI staining, and transmission electron microscopy, we integrate event-based and age-based landmarks to define nine embryonic stages (S1-S9) and a first postembryonic, premanca stage (S10). We further provide a coarse description of postembryonic development through mancae and juveniles to sexual maturity and provide a practical guide for non-invasive staging of embryos within intact marsupia of live gravid females. This refined staging framework resolves ambiguities in previous descriptions and provides a precise temporal backbone for comparative and functional evo-devo studies of arthropods. It enables detailed investigations of developmental divergence between surface and cave populations, facilitates field-based studies of reproductive phenology and environmental effects on development, with potential applications in climate-change biology. Together, these advances establish A. aquaticus as a powerful model for studying the evolution of development across environmental gradients and adaptive transitions. CI - Copyright (c) 2026 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Bogataj, Urban AU - Bogataj U AD - University of Ljubljana, Biotechnical faculty, Department of Biology, Vecna pot 111, Ljubljana, SI-1000, Slovenia. Electronic address: urban.bogataj@bf.uni-lj.si. FAU - Mrak, Polona AU - Mrak P AD - University of Ljubljana, Biotechnical faculty, Department of Biology, Vecna pot 111, Ljubljana, SI-1000, Slovenia. FAU - Znidarsic, Nada AU - Znidarsic N AD - University of Ljubljana, Biotechnical faculty, Department of Biology, Vecna pot 111, Ljubljana, SI-1000, Slovenia. FAU - Fiser, Ziga AU - Fiser Z AD - University of Ljubljana, Biotechnical faculty, Department of Biology, Vecna pot 111, Ljubljana, SI-1000, Slovenia. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Arthropod Struct Dev JT - Arthropod structure & development JID - 100972232 SB - IM OTO - NOTNLM OT - Arthropods OT - Asellus aquaticus OT - Crustacea OT - Embryonic development OT - Isopoda OT - Staging system COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:08 PHST- 2026/07/13 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:08 [entrez] AID - S1467-8039(26)00068-X [pii] AID - 10.1016/j.asd.2026.101574 [doi] PST - aheadofprint SO - Arthropod Struct Dev. 2026 Oct 27;95:101574. doi: 10.1016/j.asd.2026.101574. PMID- 42945807 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-2542 (Electronic) IS - 0378-1135 (Linking) VI - 323 DP - 2026 Oct 24 TI - A pilot study of tenofovir alafenamide fumarate as a promising therapeutic candidate for feline leukemia virus infection. PG - 111271 LID - S0378-1135(26)00408-6 [pii] LID - 10.1016/j.vetmic.2026.111271 [doi] AB - Feline leukemia virus (FeLV) is a major pathogenic gammaretrovirus causing severe immunosuppression, hematopoietic disorders, and high mortality in cats worldwide. Currently, effective and well-tolerated long-term antiviral therapies for clinical FeLV infection remain limited. This study evaluated the anti-FeLV efficacy, molecular mechanism, pharmacokinetics, and safety of tenofovir alafenamide fumarate (TAF), a second-generation tenofovir prodrug. The antiviral activity of the earlier-generation prodrug tenofovir disoproxil fumarate (TDF) was also evaluated in vitro for comparison. Molecular docking and 100-ns molecular dynamics simulations revealed that tenofovir diphosphate (TFV-DP), the common active metabolite of TAF and TDF, stably binds to the active pocket of FeLV reverse transcriptase (FeLV-RT) via stable hydrogen bonds with a binding free energy of -50 kcal/mol, competitively blocking viral DNA synthesis. In F81 cells, both TAF and TDF showed dose-dependent anti-FeLV activity with EC(50) values of 284.1 nM and 98.2 nM, respectively. TAF exhibited markedly lower cytotoxicity (CC(50) = 1781.3 muM) than TDF (CC(50) = 142.7 muM), with selectivity indices of 6272 and 1456, respectively. Given the poor bioavailability of the parent tenofovir and the safety limitations associated with TDF, TAF was selected for in vivo evaluation. In cats experimentally infected with FeLV, 45 days of oral TAF (1.5, 5, 15 mg/kg) reduced viral loads in lungs, lymph nodes, and spleen, and suppressed viremia and oronasal viral shedding in a dose-dependent manner. Pharmacokinetic analysis showed rapid oral absorption and dose-proportional exposure. Serum biochemistry confirmed no obvious hepatic or renal toxicity during the treatment period. Collectively, these findings provide preliminary evidence that TAF can suppress FeLV replication and viral shedding in experimentally infected cats and support further evaluation of TAF as a potential therapeutic option for FeLV infection. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Li, Weihui AU - Li W AD - National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China; Hubei Hongshan Laboratory, Wuhan, Hubei, 430070, PR China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, PR China. FAU - Yan, Quanhui AU - Yan Q AD - National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China; Hubei Hongshan Laboratory, Wuhan, Hubei, 430070, PR China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, PR China. FAU - Peng, Jiajia AU - Peng J AD - Wuhan Keqian Biology Co., Ltd, Wuhan, Hubei, PR China. FAU - Li, Luying AU - Li L AD - National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China; Hubei Hongshan Laboratory, Wuhan, Hubei, 430070, PR China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, PR China. FAU - Zhang, Yan AU - Zhang Y AD - National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China; Hubei Hongshan Laboratory, Wuhan, Hubei, 430070, PR China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, PR China. FAU - Ye, Zijun AU - Ye Z AD - National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China; Hubei Hongshan Laboratory, Wuhan, Hubei, 430070, PR China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, PR China. FAU - Xiong, Junyao AU - Xiong J AD - National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China; Hubei Hongshan Laboratory, Wuhan, Hubei, 430070, PR China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, PR China. FAU - Li, Qiuyan AU - Li Q AD - National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China; Hubei Hongshan Laboratory, Wuhan, Hubei, 430070, PR China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, PR China. FAU - Si, Youhui AU - Si Y AD - National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China; Hubei Hongshan Laboratory, Wuhan, Hubei, 430070, PR China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, PR China; Frontiers Science Center for Animal Breeding and Sustainable Production, PR China. Electronic address: youhui@mail.hzau.edu.cn. FAU - Cao, Shengbo AU - Cao S AD - National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China; Hubei Hongshan Laboratory, Wuhan, Hubei, 430070, PR China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Wuhan, Hubei, 430070, PR China. Electronic address: sbcao@mail.hzau.edu.cn. LA - eng PT - Journal Article DEP - 20261024 PL - Netherlands TA - Vet Microbiol JT - Veterinary microbiology JID - 7705469 SB - IM OTO - NOTNLM OT - Drug repurposing OT - FeLV OT - Feline antiviral therapy OT - Reverse transcriptase inhibitor OT - Tenofovir alafenamide fumarate COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:08 PHST- 2026/06/12 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:08 [entrez] AID - S0378-1135(26)00408-6 [pii] AID - 10.1016/j.vetmic.2026.111271 [doi] PST - aheadofprint SO - Vet Microbiol. 2026 Oct 24;323:111271. doi: 10.1016/j.vetmic.2026.111271. PMID- 42945800 OWN - NLM STAT- Publisher LR - 20261027 IS - 1090-2104 (Electronic) IS - 0006-291X (Linking) VI - 841 DP - 2026 Oct 27 TI - PCB-118 increases PDE4D abundance and thermal stability in bladder cancer cells: An integrative toxicogenomic and cellular study. PG - 154810 LID - S0006-291X(26)01576-7 [pii] LID - 10.1016/j.bbrc.2026.154810 [doi] AB - Polychlorinated biphenyls (PCBs) are persistent environmental contaminants with potential carcinogenic effects, but their molecular associations with bladder cancer remain poorly understood. Here, we integrated toxicogenomic, transcriptomic, single-cell, structural, and cellular analyses to identify candidate proteins responsive to PCB-118 in bladder cancer. Differentially expressed genes and weighted gene co-expression network modules derived from GSE13507 were integrated with curated PCB-associated genes, followed by random forest and least absolute shrinkage and selection operator analyses. PDE4D, SH2D3C, and NRG1 were consistently prioritized and showed concordant expression patterns in an independent cohort. Single-cell transcriptomic analyses revealed distinct expression distributions across tumor-associated stromal, endothelial, epithelial, and immune cell populations. Molecular docking predicted favorable interactions between PCB-118 and all three candidate proteins, with PDE4D showing the lowest binding energy. During 100-ns molecular dynamics simulations, the PCB-118-PDE4D complex exhibited the most stable and compact structural behavior. In T24 bladder cancer cells, exposure to PCB-118 at 5-20 nM increased PDE4D protein abundance. An exploratory cellular thermal shift assay showed an apparent increase in PDE4D thermal stability following PCB-118 treatment, providing preliminary support for a possible interaction at the cellular level. These findings identify PDE4D as a candidate PCB-118-responsive protein in bladder cancer cells and provide a basis for investigating its functional contribution to PCB-associated cellular effects. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Wang, Hao AU - Wang H AD - School of Clinical Medicine, Shandong Second Medical University, Weifang, Shandong, People's Republic of China. FAU - Wang, Bin AU - Wang B AD - School of Clinical Medicine, Shandong Second Medical University, Weifang, Shandong, People's Republic of China. FAU - Liu, Hongquan AU - Liu H AD - Department of Urology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, NO. 20 East Yuhuangding Road, Yantai, Shandong, 264000, People's Republic of China. FAU - Wu, Jitao AU - Wu J AD - Department of Urology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, NO. 20 East Yuhuangding Road, Yantai, Shandong, 264000, People's Republic of China. Electronic address: wjturology@163.com. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Biochem Biophys Res Commun JT - Biochemical and biophysical research communications JID - 0372516 SB - IM OTO - NOTNLM OT - Bladder cancer OT - Cellular thermal shift assay OT - Molecular dynamics OT - PCB-118 OT - PDE4D OT - Toxicogenomics COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:08 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/25 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:08 [entrez] AID - S0006-291X(26)01576-7 [pii] AID - 10.1016/j.bbrc.2026.154810 [doi] PST - aheadofprint SO - Biochem Biophys Res Commun. 2026 Oct 27;841:154810. doi: 10.1016/j.bbrc.2026.154810. PMID- 42945799 OWN - NLM STAT- Publisher LR - 20261027 IS - 1090-2104 (Electronic) IS - 0006-291X (Linking) VI - 841 DP - 2026 Oct 17 TI - Proximity labeling technologies for multiscale spatial proteomics: from organelle contact sites to intercellular and inter-organ communication. PG - 154761 LID - S0006-291X(26)01527-5 [pii] LID - 10.1016/j.bbrc.2026.154761 [doi] AB - Proximity labeling (PL) converts molecular proximity into covalent tags that can be enriched and identified by mass spectrometry. Faster ligases and engineered peroxidases have broadened the applications of PL. Genetically encoded split systems add spatial control, helping extend bait-centered mapping to organelle interfaces, intercellular interactions and extracellular communication. This Review discusses how these approaches address two related questions: which proteins occupy a defined interface, and how proteins move from their site of origin to a recipient compartment, cell or tissue. We examine how interface-associated proteins are identified and how source-destination labeling follows protein movement. We then consider the further evidence needed to explain their mechanisms and biological functions. By relating experimental design to the biological boundary under study, this Review offers practical guidance for selecting PL strategies across multiple scales. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Chen, Danhua AU - Chen D AD - Department of Pathophysiology, Zunyi Medical University, Zunyi, 563000, China; Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, 100730, China. FAU - Zhao, Hailong AU - Zhao H AD - Department of Pathophysiology, Zunyi Medical University, Zunyi, 563000, China. Electronic address: hailongzhao@zmu.edu.cn. FAU - Zhao, Hongmei AU - Zhao H AD - Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, 100730, China; Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, 610052, China. Electronic address: hongmeizhao@ibms.pumc.edu.cn. FAU - Wang, Jing AU - Wang J AD - Department of Pathophysiology, Zunyi Medical University, Zunyi, 563000, China; Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, Beijing, 100730, China. Electronic address: wangjing@ibms.pumc.edu.cn. LA - eng PT - Journal Article PT - Review DEP - 20261017 PL - United States TA - Biochem Biophys Res Commun JT - Biochemical and biophysical research communications JID - 0372516 SB - IM OTO - NOTNLM OT - Intercellular communication OT - Organelle contact sites OT - Protein trafficking OT - Proximity labeling OT - Secretome OT - Spatial proteomics COIS- Declaration of competing interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:08 PHST- 2026/08/06 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:08 [entrez] AID - S0006-291X(26)01527-5 [pii] AID - 10.1016/j.bbrc.2026.154761 [doi] PST - aheadofprint SO - Biochem Biophys Res Commun. 2026 Oct 17;841:154761. doi: 10.1016/j.bbrc.2026.154761. PMID- 42945791 OWN - NLM STAT- Publisher LR - 20261027 IS - 1879-2448 (Electronic) IS - 0043-1354 (Linking) VI - 309 IP - Pt A DP - 2026 Oct 18 TI - Bioinspired cascade‑functional hydrogel membranes with electrostatically assembled heterogeneous domains for synergistic uranium(VI) capture. PG - 127166 LID - S0043-1354(26)01838-5 [pii] LID - 10.1016/j.watres.2026.127166 [doi] AB - Efficient capture of U(VI) hinges not solely on high coordination-group density, but critically on the concerted regulation of site accessibility, ion partitioning, and network hydration. Drawing inspiration from the functional specialization and cooperative interplay of heterogeneous units in biological immune responses, we develop an electrostatically assisted precursor-assembly strategy to construct a ternary poly(acrylic acid)-poly(amidoxime)-poly(styrenesulfonic acid) (PAA-PAO-PSS) hydrogel membrane with differentiated yet synergistic functional domains. Gradual PSS addition into PAO dispersion suppresses excessive local complexation and aggregation, yielding a homogeneous precursor. PSS-enriched domains regulate hydration, the negative microenvironment, and ion competition, while PAO domains provide high-affinity N/O sites, establishing a cascade process of ion pre-screening, hydrated transport, and coordination anchoring within a crosslinked PAA network. The representative PSS60% membrane achieves a Langmuir capacity of 609 mg g(-)(1) and retains 89.1% capacity after five cycles, with favorable ion selectivity. Spectroscopic, XAFS, and DFT analyzes confirm that PSS modulates hydration and partitioning, PAA carboxyl groups assist, and PAO amidoximes chelate U(VI). This work establishes precursor-pathway engineering as an effective strategy for constructing heterogeneous adsorption microenvironments and provides a general design principle for high-performance radionuclide adsorbents. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Mi, Zhiming AU - Mi Z AD - Jiangxi Provincial Key Laboratory of Functional Organic Polymers, East China University of Technology, Nanchang, 330013, PR China. FAU - Huang, Yangyang AU - Huang Y AD - Jiangxi Provincial Key Laboratory of Functional Organic Polymers, East China University of Technology, Nanchang, 330013, PR China. FAU - Lei, Guangqiu AU - Lei G AD - Jiangxi Provincial Key Laboratory of Functional Organic Polymers, East China University of Technology, Nanchang, 330013, PR China. FAU - Yin, Mingli AU - Yin M AD - Jiangxi Provincial Key Laboratory of Functional Organic Polymers, East China University of Technology, Nanchang, 330013, PR China. FAU - Wang, Kangfei AU - Wang K AD - Jiangxi Provincial Key Laboratory of Functional Organic Polymers, East China University of Technology, Nanchang, 330013, PR China. FAU - Wan, Jiaxu AU - Wan J AD - Jiangxi Provincial Key Laboratory of Functional Organic Polymers, East China University of Technology, Nanchang, 330013, PR China. FAU - Zhu, Tianliang AU - Zhu T AD - Jiangxi Provincial Key Laboratory of Functional Organic Polymers, East China University of Technology, Nanchang, 330013, PR China. FAU - Yuan, Dingzhong AU - Yuan D AD - Jiangxi Provincial Key Laboratory of Functional Organic Polymers, East China University of Technology, Nanchang, 330013, PR China. FAU - Liu, Zhixiao AU - Liu Z AD - Jiangxi Provincial Key Laboratory of Functional Organic Polymers, East China University of Technology, Nanchang, 330013, PR China. Electronic address: lzx@ecut.edu.cn. LA - eng PT - Journal Article DEP - 20261018 PL - England TA - Water Res JT - Water research JID - 0105072 SB - IM OTO - NOTNLM OT - Bioinspired OT - Electrostatic-assembled OT - Heterogeneous domains OT - Hydrogel membrane OT - Uranium (VI) capture COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:08 PHST- 2026/08/23 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:08 [entrez] AID - S0043-1354(26)01838-5 [pii] AID - 10.1016/j.watres.2026.127166 [doi] PST - aheadofprint SO - Water Res. 2026 Oct 18;309(Pt A):127166. doi: 10.1016/j.watres.2026.127166. PMID- 42945790 OWN - NLM STAT- Publisher LR - 20261027 IS - 1618-0372 (Electronic) IS - 0065-1281 (Linking) VI - 128 IP - 4 DP - 2026 Oct 27 TI - Mining key differentially expressed genes and construction of a prognostic risk score model for colorectal cancer based on bioinformatics. PG - 152377 LID - S0065-1281(26)00062-0 [pii] LID - 10.1016/j.acthis.2026.152377 [doi] AB - Colorectal cancer (CRC) remains the third most common malignancy and the second leading cause of cancer-related death worldwide, with limited biomarkers available for accurate prognostic stratification. This study integrates multi-cohort transcriptomic data from four GEO datasets (GSE39582, GSE17536, GSE14333, GSE33113; n = 1123) and the TCGA-CRC cohort (n = 621) to identify key differentially expressed genes and construct a validated prognostic risk score model. Differential expression analysis using the limma framework identified 642 upregulated and 588 downregulated genes (|log2FC|>1, FDR<0.05). Weighted gene co-expression network analysis (WGCNA) extracted hub genes from modules significantly correlated with overall survival. LASSO Cox regression then reduced the hub gene set to an eight-gene prognostic signature (MMP1, AURKA, TOP2A, CDH1, COL1A1, CLDN1, ETV4, CDX2) that stratified patients into high- and low-risk groups with significantly different survival outcomes (p < 0.001, log-rank test). The model achieved AUCs of 0.824, 0.796, and 0.768 for 1-, 3-, and 5-year overall survival in the training cohort and was validated in three independent datasets. High-risk patients exhibited immunosuppressive microenvironments (elevated M2 macrophages, Tregs) and increased resistance to standard chemotherapy agents. Integration of the risk score with clinical variables improved prognostic accuracy (C-index 0.812). These findings offer a robust bioinformatics-derived prognostic tool with implications for immunotherapy patient selection and personalized treatment planning in CRC. CI - Copyright (c) 2026 Elsevier GmbH. All rights reserved. FAU - Liang, Likun AU - Liang L AD - Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, 150000, China. Electronic address: llk186297263152025@163.com. FAU - Lin, Han AU - Lin H AD - Department of Hepatic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, 150000, China. Electronic address: linhandoc2009@163.com. FAU - Jun, Lina AU - Jun L AD - Guilin University of Aerospace Technology, Guilin, 541004, China. LA - eng PT - Journal Article DEP - 20261027 PL - Germany TA - Acta Histochem JT - Acta histochemica JID - 0370320 SB - IM OTO - NOTNLM OT - Bioinformatics OT - Colorectal cancer OT - Differentially expressed genes OT - Immune infiltration OT - LASSO Cox regression OT - Overall survival OT - Prognostic risk score OT - Tumor microenvironment OT - WGCNA COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:08 PHST- 2026/06/14 00:00 [received] PHST- 2026/09/08 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:08 [entrez] AID - S0065-1281(26)00062-0 [pii] AID - 10.1016/j.acthis.2026.152377 [doi] PST - aheadofprint SO - Acta Histochem. 2026 Oct 27;128(4):152377. doi: 10.1016/j.acthis.2026.152377. PMID- 42945772 OWN - NLM STAT- Publisher LR - 20261027 IS - 1532-1967 (Electronic) IS - 0305-7372 (Linking) VI - 151 DP - 2026 Oct 24 TI - Immune checkpoint inhibitors in platinum-resistant ovarian cancer: Clinical disappointments, biological lessons, and the reopening of therapeutic opportunities. PG - 103237 LID - S0305-7372(26)00151-9 [pii] LID - 10.1016/j.ctrv.2026.103237 [doi] AB - Immune checkpoint inhibitors (ICIs) have transformed the treatment landscape of several malignancies; however, their efficacy in platinum-resistant ovarian cancer (PROC) remains limited. The immunosuppressive tumor microenvironment (TME) in ovarian cancer constitutes a major barrier to effective antitumor immunity, prompting the development of various strategies to enhance responsiveness to ICIs. This narrative review examines current therapeutic approaches targeting the TME to overcome resistance to ICIs in PROC, with a focus on angiogenesis, innate immunity, and adaptive immune pathways. Despite a strong biological rationale, most chemotherapy-free ICI-based combinations targeting specific immune or stromal components have shown limited and inconsistent clinical benefit. The most notable clinical activity has been observed when ICIs are combined with chemotherapy, particularly in regimens that include anti-angiogenic agents. Recent positive results from a single phase 3 trial evaluating pembrolizumab in combination with weekly paclitaxel, with or without bevacizumab, mark a notable deviation from the predominantly negative outcomes of earlier studies. Nevertheless, it remains uncertain whether this result represents a true paradigm shift or a context-specific effect. Currently, no validated predictive biomarkers exist to reliably identify patients most likely to benefit from ICI-based strategies. The discrepancy between the isolated success of chemo-immunotherapy combinations and the failure of most previous trials highlights the need for further investigation into TME biology. A more comprehensive understanding of TME dynamics and the development of biomarker-driven patient selection strategies will be essential to define the role of ICIs in this challenging clinical context. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Giudice, Elena AU - Giudice E AD - Division of Gynecologic Oncology, Humanitas San Pio X, Milan, Italy; Department of Biomedical Sciences, Humanitas University, Via Rita Levi Montalcini 4, 20072 Pieve Emanuele, Milan, Italy. Electronic address: elena.giudice@sanpiox.humanitas.it. FAU - Spinazzola, Andrea AU - Spinazzola A AD - Phase 1 Unit, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy. Electronic address: andrea.spinazzola@guest.policlinicogemelli.it. FAU - Floudas, Charalampos S AU - Floudas CS AD - Center for Immuno-Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. Electronic address: charalampos.floudas@nih.gov. FAU - Iannantuono, Giovanni Maria AU - Iannantuono GM AD - Phase 1 Research Center, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy. Electronic address: giovannimaria.iannantuono@irccs-sangerardo.it. FAU - Sabetta, Giulia AU - Sabetta G AD - Department of Woman, Child and Public Health, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy. Electronic address: giulia.sabetta@guest.policlinicogemelli.it. FAU - Ghizzoni, Viola AU - Ghizzoni V AD - Department of Woman, Child and Public Health, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy. Electronic address: viola.ghizzoni@policlinicogemelli.it. FAU - Rapisarda, Elvira AU - Rapisarda E AD - Institute of Obstetrics and Gynecology, Universita Cattolica del Sacro Cuore, Rome, Italy. FAU - Giustozzi, Alessandra AU - Giustozzi A AD - Institute of Obstetrics and Gynecology, Universita Cattolica del Sacro Cuore, Rome, Italy. FAU - Sabatucci, Ilaria AU - Sabatucci I AD - Division of Gynecologic Oncology, Humanitas San Pio X, Milan, Italy. Electronic address: ilaria.sabatucci@sanpiox.humanitas.it. FAU - Paderno, Mariachiara AU - Paderno M AD - Division of Gynecologic Oncology, Humanitas San Pio X, Milan, Italy. Electronic address: mariachiara.paderno@sanpiox.humanitas.it. FAU - Turinetto, Margherita AU - Turinetto M AD - Division of Gynecologic Oncology, Humanitas San Pio X, Milan, Italy. Electronic address: margherita.turinetto@sanpiox.humanitas.it. FAU - Salutari, Vanda AU - Salutari V AD - Department of Woman, Child and Public Health, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy. Electronic address: vanda.salutari@policlinicogemelli.it. FAU - Daniele, Gennaro AU - Daniele G AD - Phase 1 Unit, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Rome, Italy. Electronic address: gennaro.daniele@policlinicogemelli.it. FAU - Cazzaniga, Marina Elena AU - Cazzaniga ME AD - Phase 1 Research Center, Fondazione IRCCS San Gerardo dei Tintori, Monza, Italy. Electronic address: marinaelena.cazzaniga@irccs-sangerardo.it. FAU - Gulley, James L AU - Gulley JL AD - Center for Immuno-Oncology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. Electronic address: gulleyj@mail.nih.gov. FAU - Lee, Jung-Min AU - Lee JM AD - Women's Malignancies Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA. FAU - Lorusso, Domenica AU - Lorusso D AD - Division of Gynecologic Oncology, Humanitas San Pio X, Milan, Italy; Department of Biomedical Sciences, Humanitas University, Via Rita Levi Montalcini 4, 20072 Pieve Emanuele, Milan, Italy. Electronic address: domenica.lorusso@hunimed.eu. LA - eng PT - Journal Article PT - Review DEP - 20261024 PL - Netherlands TA - Cancer Treat Rev JT - Cancer treatment reviews JID - 7502030 SB - IM OTO - NOTNLM OT - Immune checkpoint inhibitors OT - biomarkers OT - platinum-resistant ovarian cancer OT - tumor microenvironment COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: DOMENICA LORUSSO reports a relationship with AstraZeneca that includes: consulting or advisory, funding grants, speaking and lecture fees, and travel reimbursement. DOMENICA LORUSSO reports a relationship with Abbvie srl that includes: consulting or advisory, funding grants, speaking and lecture fees, and travel reimbursement. DOMENICA LORUSSO reports a relationship with GSK that includes: consulting or advisory, funding grants, speaking and lecture fees, and travel reimbursement. DOMENICA LORUSSO reports a relationship with Incyte that includes: funding grants. DOMENICA LORUSSO reports a relationship with MSD Italy that includes: consulting or advisory, funding grants, speaking and lecture fees, and travel reimbursement. DOMENICA LORUSSO reports a relationship with Corcept Therapeutics Inc. that includes: consulting or advisory, funding grants, and travel reimbursement. DOMENICA LORUSSO reports a relationship with Genmab AS that includes: consulting or advisory, speaking and lecture fees, and travel reimbursement. DOMENICA LORUSSO reports a relationship with pharma& that includes: consulting or advisory. DOMENICA LORUSSO reports a relationship with Regeneron that includes: consulting or advisory, speaking and lecture fees, and travel reimbursement. ELENA GIUDICE reports a relationship with MSD Italy that includes: consulting or advisory, speaking and lecture fees, and travel reimbursement. ELENA GIUDICE reports a relationship with GSK that includes: speaking and lecture fees and travel reimbursement. ELENA GIUDICE reports a relationship with AstraZeneca that includes: speaking and lecture fees. ELENA GIUDICE reports a relationship with AbbVie Srl that includes: speaking and lecture fees and travel reimbursement. ELENA GIUDICE reports a relationship with Daiichi Sankyo Inc. that includes: consulting or advisory. ELENA GIUDICE reports a relationship with Amgen that includes: consulting or advisory. VIOLA GHIZZONI reports a relationship with AbbVie Srl that includes: speaking and lecture fees. VIOLA GHIZZONI reports a relationship with MSD Italy that includes: travel reimbursement. VIOLA GHIZZONI reports a relationship with GSK that includes: travel reimbursement. VIOLA GHIZZONI reports a relationship with AstraZeneca that includes: travel reimbursement. VANDA SALUTARI reports a relationship with MSD Italy that includes: speaking and lecture fees. VANDA SALUTARI reports a relationship with GSK that includes: speaking and lecture fees. VANDA SALUTARI reports a relationship with AstraZeneca that includes: speaking and lecture fees. VANDA SALUTARI reports a relationship with AbbVie Srl that includes: speaking and lecture fees. VANDA SALUTARI reports a relationship with pharma& that includes: speaking and lecture fees. JUNG-MIN LEE reports a relationship with GSK that includes: employment. GIOVANNI MARIA IANNANTUONO reports a relationship with Eli Lilly and Company that includes: travel reimbursement. ILARIA SABATUCCI reports a relationship with AstraZeneca that includes: consulting or advisory, employment, speaking and lecture fees, and travel reimbursement. ILARIA SABATUCCI reports a relationship with MSD Italy that includes: consulting or advisory, speaking and lecture fees, and travel reimbursement. ILARIA SABATUCCI reports a relationship with GSK that includes: consulting or advisory, speaking and lecture fees, and travel reimbursement. ILARIA SABATUCCI reports a relationship with pharma& that includes: consulting or advisory, speaking and lecture fees, and travel reimbursement. GENNARO DANIELE reports a relationship with Bayer that includes: consulting or advisory and speaking and lecture fees. GENNARO DANIELE reports a relationship with AstraZeneca that includes: speaking and lecture fees and travel reimbursement. GENNARO DANIELE reports a relationship with Gilead that includes: speaking and lecture fees. GENNARO DANIELE reports a relationship with Roche that includes: travel reimbursement. MARIACHIARA PADERNO reports a relationship with AbbVie Srl that includes: consulting or advisory. MARIACHIARA PADERNO reports a relationship with pharma& that includes: consulting or advisory and travel reimbursement. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:07 PHST- 2026/05/03 00:00 [received] PHST- 2026/08/01 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:07 [entrez] AID - S0305-7372(26)00151-9 [pii] AID - 10.1016/j.ctrv.2026.103237 [doi] PST - aheadofprint SO - Cancer Treat Rev. 2026 Oct 24;151:103237. doi: 10.1016/j.ctrv.2026.103237. PMID- 42945764 OWN - NLM STAT- Publisher LR - 20261027 IS - 1877-783X (Electronic) IS - 1877-7821 (Linking) VI - 105 DP - 2026 Oct 27 TI - A policy evaluation of Australia's commercial solarium ban: A 14-year regression discontinuity analysis of melanoma incidence, mortality and clinical severity. PG - 103265 LID - S1877-7821(26)00281-X [pii] LID - 10.1016/j.canep.2026.103265 [doi] AB - BACKGROUND: Australia prohibited commercial solariums in 2015 to reduce exposure to artificial ultraviolet (UV) radiation, an established and preventable cause of melanoma. However, evidence of population-level effects on melanoma incidence, mortality, and clinical severity remains limited. OBJECTIVE: To evaluate whether the commercial solarium ban was associated with immediate and longer-term changes in melanoma incidence, mortality, and clinical severity nationally and across states and demographic, geographic, and tumour-thickness subgroups. METHODS: We conducted a quasi-experimental regression discontinuity study using three population-based datasets from the Australian Institute of Health and Welfare. Analyses examined national melanoma incidence (1982-2021), mortality (1971-2021), state-specific trends across six states accounting for Western Australia's 2016 implementation, and clinical severity using Breslow thickness and other histopathological indicators. Parametric ordinary least squares models with heteroskedasticity-robust standard errors were complemented by non-parametric local polynomial analyses. Robustness was assessed using alternative bandwidths, kernel specifications, and placebo cutoffs. RESULTS: At the national level, the commercial solarium ban was associated with an estimated immediate reduction of 3.86 melanoma cases per 100,000 population and 2.28 melanoma deaths per 100,000 population. However, neither reduction was statistically significant (p = 0.493 and p = 0.171, respectively). Following the ban, the annual incidence trend decreased by 2.00 cases per 100,000 among males (p < 0.001) and by 3.62 cases per 100,000 among adults aged 60-69 years (p < 0.001). In the same age group, the annual mortality trend decreased by 0.59 deaths per 100,000 (p < 0.001). Declining incidence trends were also observed for thin melanomas (2-4 mm), while melanomas thicker > 4 mm exhibited an immediate reduction following the ban. Queensland and Western Australia experienced declining incidence trends, with a corresponding decline in mortality trends in Western Australia. CONCLUSION: The ban was not associated with an abrupt nationwide reduction in melanoma incidence or mortality but coincided with improvements in selected demographic, geographic, and tumour-thickness groups. These heterogeneous patterns suggest a potential gradual policy effect rather than an immediate population-wide decline. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Tohan, Mortuja Mahamud AU - Tohan MM AD - Health Economics and Health Technology Assessment Unit, National Health and Medical Research Council Clinical Trials Centre, Faculty of Medicine and Health, The University of Sydney, 119-143 Missenden Rd, Level 6, Camperdown, NSW, 2050, Australia; School of General Education, BRAC University, Dhaka, Bangladesh. Electronic address: mtoh9195@uni.sydney.edu.au. FAU - Mahumud, Rashidul Alam AU - Mahumud RA AD - School of General Education, BRAC University, Dhaka, Bangladesh. Electronic address: rashed.mahumud@sydney.edu.au. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Cancer Epidemiol JT - Cancer epidemiology JID - 101508793 SB - IM OTO - NOTNLM OT - Artificial ultraviolet radiation OT - Australia OT - Breslow thickness OT - Melanoma OT - Public health policy OT - Regression discontinuity OT - Solarium ban COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:07 PHST- 2026/08/23 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:07 [entrez] AID - S1877-7821(26)00281-X [pii] AID - 10.1016/j.canep.2026.103265 [doi] PST - aheadofprint SO - Cancer Epidemiol. 2026 Oct 27;105:103265. doi: 10.1016/j.canep.2026.103265. PMID- 42945754 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-5487 (Electronic) IS - 0188-4409 (Linking) VI - 58 IP - 1 DP - 2026 Oct 27 TI - Validation of the Healthy Complementary Feeding Practices Index: A Novel Approach for Assessing Infant Feeding in Mexico. PG - 103535 LID - S0188-4409(26)00157-8 [pii] LID - 10.1016/j.arcmed.2026.103535 [doi] AB - OBJECTIVE: To develop and validate the Healthy Complementary Feeding Practices Index (HCFPI), a novel tool designed to assess adherence to Mexico's national dietary guidelines and inform the design of interventions to prevent malnutrition in infants aged 6-24 months. METHODS: The HCFPI was constructed using a literature-based selection of components, indicators, and scoring criteria. Content validity was evaluated using the Delphi method with experts. Internal consistency (Cronbach's alpha) and concurrent validity with dietary intake were examined in a cross-sectional sample from a Mexican birth cohort at 6, 12, and 24 months. RESULTS: The HCFPI comprises 13 components and 15 items that reflect key CF dimensions, and it achieved "acceptable" expert consensus during content validation. The index demonstrated "acceptable" internal consistency (alpha = 0.70) and meaningful correlations with optimal Complementary Feeding (CF) practices, such as breastfeeding, the timely introduction of solids, and the avoidance of both unhealthy salty foods and follow-up formulas. Furthermore, higher HCFPI scores were associated with better dietary quality, including higher fiber intake, greater protein and vitamin B12 adequacy, and lower added sugar intake. CONCLUSION: The HCFPI is a valid, reliable, and practical tool for evaluating adherence to CF recommendations and dietary quality in young children. Its framework offers a model that could be adapted to create similar indices in other countries using evidence-based national dietary guidelines. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Tercero-Gomez, Francia AU - Tercero-Gomez F AD - National Institute of Public Health, Center for Nutrition and Health Research. FAU - Ramirez-Silva, Ivonne AU - Ramirez-Silva I AD - National Institute of Public Health, Center for Nutrition and Health Research. Electronic address: ciarmir@insp.mx. FAU - Rivera, Juan A AU - Rivera JA AD - National Institute of Public Health, Center for population Health Research. FAU - Fuentes-Garcia, Ruth AU - Fuentes-Garcia R AD - Department of Mathematics, Faculty of Science, National Autonomus of Mexico, Mexico City, Mexico. FAU - Perez-Escamilla, Rafael AU - Perez-Escamilla R AD - Yale School of Public Health. Yale University, New Haven, CT. USA. FAU - Olvera-Mayorga, Gabriela AU - Olvera-Mayorga G AD - National Institute of Public Health, Center for Nutrition and Health Research; Nutrition Academic Area, Institute of Health Sciences, Autonomous University of the State of Hidalgo, Mexico. FAU - Barragan-Vazquez, Sofia AU - Barragan-Vazquez S AD - National Institute of Public Health, Center for Nutrition and Health Research; Nutritional Epidemiology Group, School of Food Science and Nutrition, University of Leeds, Woodhouse, Leeds, UK. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Arch Med Res JT - Archives of medical research JID - 9312706 SB - IM OTO - NOTNLM OT - Complementary feeding OT - Diet quality OT - Index OT - Infant feeding practices OT - Validation EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:07 PHST- 2025/10/02 00:00 [received] PHST- 2026/08/10 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:07 [entrez] AID - S0188-4409(26)00157-8 [pii] AID - 10.1016/j.arcmed.2026.103535 [doi] PST - aheadofprint SO - Arch Med Res. 2026 Oct 27;58(1):103535. doi: 10.1016/j.arcmed.2026.103535. PMID- 42945752 OWN - NLM STAT- Publisher LR - 20261027 IS - 1878-013X (Electronic) IS - 1878-013X (Linking) VI - 89 DP - 2026 Oct 27 TI - Developing an introductory training programme for newly employed nurses in the ambulance service through participatory action research. PG - 101919 LID - S1755-599X(26)00178-3 [pii] LID - 10.1016/j.ienj.2026.101919 [doi] AB - BACKGROUND: Newly employed nurses entering the ambulance service face clinical, organisational and cultural challenges. Although transition-related support needs have been described, less is known about how such knowledge can be translated into organisationally feasible introductory training programmes. AIM: To explore how organisational and pedagogical challenges in introductory training for newly employed nurses were identified, interpreted, negotiated and translated into a revised programme through participatory action research (PAR). METHODS: A qualitative PAR design was conducted between 2020 and 2024 in one Swedish ambulance service organisation. Newly employed nurses, clinical supervisors, the management group (total duration 2 h and 14 min) and the research group contributed at different phases and levels of participation. The primary analytic material comprised two video-recorded management group meetings, field notes, and researcher reflections. Previously analysed interview, focus group and questionnaire findings were integrated as experiential and evaluative material. Narrative process analysis reconstructed how identified challenges were interpreted, negotiated, and translated into programme interventions, and how these were reconsidered following implementation. RESULTS: Four interrelated challenges were consolidated: a theory-heavy programme structure, variation in supervision, limited leadership connection and role ambiguity. Organisational interpretation and negotiation translated these into three interconnected interventions: restructuring theory and practice, preparing selected clinical supervisors and integrating peer learning during clinical placement, with leadership connection and role clarification embedded across the programme. Reflection on implementation indicated that theory-practice integration, supervision, leadership and peer learning were interdependent and required continued organisational and pedagogical support. CONCLUSION: PAR provided a structured process for moving from empirical knowledge about professional transition to organisationally embedded and contextually feasible programme development. Supporting newly employed nurses requires alignment between programme design, supervision, leadership and workplace learning. REGISTRATION: Not registered. CI - Copyright (c) 2026 The Authors. Published by Elsevier Ltd.. All rights reserved. FAU - Jepsen, Klara AU - Jepsen K AD - Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, Stockholm, Sweden; Samariten Ambulans AB, Stockholm, Sweden. Electronic address: klara.jepsen@ki.se. FAU - Wihlborg, Jonas AU - Wihlborg J AD - School of health and welfare, Dalarna University, Falun, Sweden. FAU - Ericsson, Christoffer AU - Ericsson C AD - Arcada University of Applied Sciences, Academy of Business and Healthcare, Helsinki, Finland. FAU - Lindstrom, Veronica AU - Lindstrom V AD - Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, Stockholm, Sweden; Department of Nursing, division of ambulance Service, Region Vasterbotten, Umea University, Umea, Sweden. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Int Emerg Nurs JT - International emergency nursing JID - 101472191 SB - IM OTO - NOTNLM OT - Ambulance service OT - Emergency medical services OT - Introductory training OT - Leadership OT - Newly employed nurses OT - Participatory action research OT - Peer learning OT - Professional transition OT - Supervision OT - Workplace learning COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:06 PHST- 2026/04/25 00:00 [received] PHST- 2026/08/17 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:06 [entrez] AID - S1755-599X(26)00178-3 [pii] AID - 10.1016/j.ienj.2026.101919 [doi] PST - aheadofprint SO - Int Emerg Nurs. 2026 Oct 27;89:101919. doi: 10.1016/j.ienj.2026.101919. PMID- 42945749 OWN - NLM STAT- Publisher LR - 20261027 IS - 1973-8102 (Electronic) IS - 0010-9452 (Linking) VI - 206 DP - 2026 Oct 21 TI - Assessing brain-behaviour coupling in non-invasive brain stimulation: Insights from reliable change indices and Monte Carlo simulations. PG - 1-15 LID - S0010-9452(26)00263-7 [pii] LID - 10.1016/j.cortex.2026.10.002 [doi] AB - Non-invasive brain stimulation (NiBS) studies frequently report exploratory brain-behaviour correlations following the application of NiBS. However, these analyses are typically underpowered and rely on ratio-based change scores with known statistical limitations. We addressed these limitations by pooling individual data from three independent studies (N = 69) and combining empirical analysis with a Monte Carlo simulation framework. All studies applied 20 Hz transcranial alternating current stimulation (tACS) targeting the pre-supplementary motor area (preSMA) and right inferior frontal gyrus (rIFG) during a stop signal task. Changes in preSMA-rIFG EEG imaginary coherency (ImCoh) and stop-signal reaction time (SSRT) were quantified using reliable change indices (RCI), z-standardised within studies for pooled mixed-effects regression. We found no meaningful association between tACS-induced ImCoh change and SSRT change (r = .013, marginal R(2) = .004), with project-wise correlations being small, non-significant, and directionally inconsistent. While sensitivity analysis using ratio-based change scores converged on the same null result (r = .014), the simulation framework revealed that ratio-based scores show near-complete failure to recover true brain-behaviour associations across all plausible effect sizes. Conversely, RCI-based scores preserved directional accuracy and provided well-calibrated Type I error control, despite moderate attenuation under realistic noise conditions. These results highlight the severe statistical limits of detecting individual-level brain-behaviour coupling following 20 Hz tACS. Rather than ruling out a true biological association, our findings demonstrate that standard sample sizes and typical measurement noise create substantial 'blindness' to realistic effect sizes, underscoring a broader need for more reliable metrics and adequately powered designs in NiBS research. CI - Copyright (c) 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved. FAU - Fujiyama, Hakuei AU - Fujiyama H AD - School of Psychology, Murdoch University, Western Australia, Australia; Centre for Healthy Ageing, Health Futures Institute, Murdoch University, Western Australia, Australia; Personalised Medicine Centre, Health Futures Institute, Murdoch University, Western Australia, Australia. Electronic address: H.Fujiyama@murdoch.edu.au. FAU - Wansbrough, Kym AU - Wansbrough K AD - School of Psychology, Murdoch University, Western Australia, Australia; Centre for Healthy Ageing, Health Futures Institute, Murdoch University, Western Australia, Australia; Personalised Medicine Centre, Health Futures Institute, Murdoch University, Western Australia, Australia; School of Medical and Health Sciences, Edith Cowan University, Perth, Australia; Centre for Precision Health, Edith Cowan University, Perth, Australia. FAU - Lebihan, Brooke AU - Lebihan B AD - School of Psychology, Murdoch University, Western Australia, Australia. FAU - Tan, Jane AU - Tan J AD - School of Psychology, Murdoch University, Western Australia, Australia; Centre for Healthy Ageing, Health Futures Institute, Murdoch University, Western Australia, Australia. FAU - Levin, Oron AU - Levin O AD - Biomedical MRI, Department of Imaging & Pathology, Group Biomedical Sciences, KU Leuven, Leuven, Belgium; Frailty & Resilience in Ageing (FRIA) research department, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Jette, Belgium; Department of Health Promotion and Rehabilitation, Lithuanian Institute of Sport Science and Innovations, Lithuanian Sports University, Kaunas, Lithuania. FAU - Mathersul, Danielle C AU - Mathersul DC AD - School of Psychology, Murdoch University, Western Australia, Australia; Centre for Healthy Ageing, Health Futures Institute, Murdoch University, Western Australia, Australia; Personalised Medicine Centre, Health Futures Institute, Murdoch University, Western Australia, Australia; War Related Illness and Injury Study Center (WRIISC), Veterans Affairs Palo Alto Health Care System, Palo Alto, CA, USA. FAU - Tang, Alexander D AU - Tang AD AD - Experimental and Regenerative Neurosciences, The University of Western Australia, Australia; Perron Institute for Neurological and Translational Sciences, Australia; Pharmacology and Toxicology Discipline, School of Biomedical Sciences, The University of Western Australia, Australia. LA - eng PT - Journal Article DEP - 20261021 PL - Italy TA - Cortex JT - Cortex; a journal devoted to the study of the nervous system and behavior JID - 0100725 SB - IM OTO - NOTNLM OT - Electroencephalography OT - Neural correlates OT - Principal component analysis OT - Response inhibition OT - Transcranial alternating current stimulation EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:06 PHST- 2026/05/27 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:06 [entrez] AID - S0010-9452(26)00263-7 [pii] AID - 10.1016/j.cortex.2026.10.002 [doi] PST - aheadofprint SO - Cortex. 2026 Oct 21;206:1-15. doi: 10.1016/j.cortex.2026.10.002. PMID- 42945748 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-5967 (Electronic) IS - 1386-6532 (Linking) VI - 187 DP - 2026 Oct 24 TI - Evaluating Nanopore sequencing as a rescue strategy for HIV-1 drug-resistance genotyping of low-yield PCR products. PG - 106023 LID - S1386-6532(26)00115-0 [pii] LID - 10.1016/j.jcv.2026.106023 [doi] AB - BACKGROUND: HIV-1 drug resistance (HIVDR) genotyping commonly requires PCR amplification before sequencing, with agarose gel electrophoresis used to assess amplification success. We investigated whether PCR products without visible gel bands could nevertheless support Nanopore sequencing and HIVDR profiling. METHODS: Thirteen protease/reverse transcriptase (PR/RT) products that had previously generated successful Sanger sequences subsequently produced no visible band following repeat PCR. These products were sequenced using Oxford Nanopore and consensus sequences and HIVDR profiles were compared with matched Sanger HIVDR profiles. FINDINGS: Nanopore sequencing generated HIV-1 PR/RT sequence from all 13 products. Across 286 drug-sample comparisons, Nanopore and Sanger HIVdb classifications agreed on susceptible versus resistant status in 74.5% (213/286), with exact agreement across the five HIVdb resistance levels in 68.5% (196/286). Discordance was directional where Nanopore detected only 53% (64/120) of Sanger-defined resistance yet rarely called resistance where Sanger did not (specificity 90%, 149/166), so errors were predominantly undercalls (56 versus 17 overcalls). Median Nanopore read quality was positively associated with Nanopore-Sanger consensus sequence identity (r = 0.67, p = 0.012), whereas read number was not significantly associated (r = 0.47, p = 0.11). CONCLUSION: PCR products without visible agarose gel bands can contain sufficient HIV-1 material for Nanopore sequencing, but sequence recovery does not necessarily ensure reliable HIVDR profiling. Resistance was frequently undercalled in poorly performing samples, potentially reflecting differences in sequence quality, amplification and consensus generation. Nanopore sequencing may provide a rescue strategy for selected low-yield PCR products, but larger studies are needed to establish quality criteria for reliable resistance interpretation. CI - Copyright (c) 2026 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Lule, Daniel Bugembe AU - Lule DB AD - London School of Hygiene and Tropical Medicine, Keppel Street, Bloomsbury, London, WC1E 7HT, United Kingdom; Medical Research Council/Uganda Virus Research Institute & London School of Hygiene and Tropical Medicine Uganda Unit, Plot 51-59 Nakiwogo Road, P. O. Box 49, Entebbe, Uganda; City St Georges University of London, Cranmer Terrace, Tooting, London, SW17 0RE, United Kingdom. FAU - Ssemwanga, Deogratius AU - Ssemwanga D AD - Medical Research Council/Uganda Virus Research Institute & London School of Hygiene and Tropical Medicine Uganda Unit, Plot 51-59 Nakiwogo Road, P. O. Box 49, Entebbe, Uganda; Uganda Virus Research Institute, Plot 51-59 Nakiwogo Road, P. O. Box 49, Entebbe, Uganda. FAU - Kaleebu, Pontiano AU - Kaleebu P AD - London School of Hygiene and Tropical Medicine, Keppel Street, Bloomsbury, London, WC1E 7HT, United Kingdom; Medical Research Council/Uganda Virus Research Institute & London School of Hygiene and Tropical Medicine Uganda Unit, Plot 51-59 Nakiwogo Road, P. O. Box 49, Entebbe, Uganda; Uganda Virus Research Institute, Plot 51-59 Nakiwogo Road, P. O. Box 49, Entebbe, Uganda. FAU - Tully, Damien C AU - Tully DC AD - London School of Hygiene and Tropical Medicine, Keppel Street, Bloomsbury, London, WC1E 7HT, United Kingdom; Medical Research Council/Uganda Virus Research Institute & London School of Hygiene and Tropical Medicine Uganda Unit, Plot 51-59 Nakiwogo Road, P. O. Box 49, Entebbe, Uganda. Electronic address: Damien.tully@lshtm.ac.uk. LA - eng PT - Journal Article DEP - 20261024 PL - Netherlands TA - J Clin Virol JT - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JID - 9815671 SB - IM OTO - NOTNLM OT - Drug resistance OT - Genotypic resistance OT - HIV-1 OT - Nanopore sequencing OT - Sanger sequencing COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:06 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:06 [entrez] AID - S1386-6532(26)00115-0 [pii] AID - 10.1016/j.jcv.2026.106023 [doi] PST - aheadofprint SO - J Clin Virol. 2026 Oct 24;187:106023. doi: 10.1016/j.jcv.2026.106023. PMID- 42945745 OWN - NLM STAT- Publisher LR - 20261027 IS - 1879-0267 (Electronic) IS - 0020-1383 (Linking) VI - 57 IP - 12 DP - 2026 Oct 15 TI - Peripheral nerve blocks reduce opioid requirements in the first 24 h following surgical fixation of acute tibial shaft fractures. PG - 113778 LID - S0020-1383(26)00765-5 [pii] LID - 10.1016/j.injury.2026.113778 [doi] AB - OBJECTIVES: To evaluate the effect of peripheral nerve blocks (PNB) on postoperative opioid use, length of stay (LOS), and incidence of acute compartment syndrome (ACS) in patients presenting with acute tibial shaft fractures treated with intramedullary nail (IMN) fixation. METHODS: Design: Retrospective cohort study Setting: Level 1 Academic Trauma Center from 2018 to 2022. Patient Selection Criteria: Patients aged 18-79 years with acute AO/OTA 42A-C tibial shaft fractures treated with IMN within 7 days of injury Outcome Measures and Comparisons: The primary outcome was postoperative opioid use, measured in morphine milligram equivalents (MME), during hospital stay, up to a maximum of 72 h after surgery. Patients who received a preoperative PNB were compared to those who did not receive a block. Secondary outcomes included postoperative LOS and incidence of ACS. RESULTS: 246 patients were eligible for analysis of which 78 (32%) received a PNB (PNB=yes) and 168 (68%) did not (PNB=no). Mean MME were significantly lower in the PNB=yes group compared to the PNB=no group at 24 h (73 vs. 138, p < 0.001). Controlling for age, Injury Severity Score (ISS), and time to surgery, a multivariable regression model demonstrated a 36% reduction in MME per hour for patients that received PNBs. There were 3 cases of ACS in PNB=No and 1 in PNB=Yes. All 3 patients without a PNB were diagnosed within 2 days of surgery and underwent uncomplicated fasciotomies. One patient with a PNB re-presented to the hospital 5 days postoperatively, underwent fasciotomies, and was found to have anterior and lateral compartment necrosis. CONCLUSION: PNBs for select acute tibial shaft fractures treated with IMN were associated with decreased opioid requirements in the first 24 h after surgery. No effect was noted beyond 24 h after surgery. Extreme vigilance is essential in patients with tibia fractures to monitor for ACS, especially when PNB is utilized. CI - Published by Elsevier Ltd. FAU - Khan, Umar A AU - Khan UA AD - Department of Orthopedic Surgery, Walter Reed National Military Medical Center, Bethesda, MD, United States. FAU - Quigley, Caitlin AU - Quigley C AD - Department of Orthopaedic Surgery, Inova Fairfax Medical Center, Falls Church, VA, United States. FAU - Schulman, Max AU - Schulman M AD - Department of Orthopaedic Surgery, Inova Fairfax Medical Center, Falls Church, VA, United States. FAU - Holland, Jordan AU - Holland J AD - Uniformed Services University of the Health Sciences, Bethesda, MD, United States. FAU - Ghidei, Senay AU - Ghidei S AD - Department of Orthopaedic Surgery, Inova Fairfax Medical Center, Falls Church, VA, United States. FAU - Kim, Andrew AU - Kim A AD - University of Virginia School of Medicine, Charlottesville, VA, United States. FAU - Schulman, Jeff AU - Schulman J AD - Department of Orthopaedic Surgery, Inova Fairfax Medical Center, Falls Church, VA, United States. FAU - Gaski, Greg AU - Gaski G AD - Department of Orthopaedic Surgery, Inova Fairfax Medical Center, Falls Church, VA, United States. Electronic address: Greg.gaski@inova.org. FAU - Hymes, Robert A AU - Hymes RA AD - Department of Orthopaedic Surgery, Inova Fairfax Medical Center, Falls Church, VA, United States. LA - eng PT - Journal Article DEP - 20261015 PL - Netherlands TA - Injury JT - Injury JID - 0226040 SB - IM OTO - NOTNLM OT - MME OT - Morphine-milliequivalents OT - Opioids OT - Peripheral nerve block OT - Regional anesthesia OT - Tibia fracture OT - Tibia intramedullary nailing COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:06 PHST- 2025/11/19 00:00 [received] PHST- 2026/07/22 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:06 [entrez] AID - S0020-1383(26)00765-5 [pii] AID - 10.1016/j.injury.2026.113778 [doi] PST - aheadofprint SO - Injury. 2026 Oct 15;57(12):113778. doi: 10.1016/j.injury.2026.113778. PMID- 42945736 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-6297 (Electronic) IS - 0001-6918 (Linking) VI - 271 DP - 2026 Oct 27 TI - Blockchain and the transparency paradox: Fear of exposure, information asymmetry, and traceability resistance in Saudi manufacturing supply chains. PG - 107971 LID - S0001-6918(26)01772-5 [pii] LID - 10.1016/j.actpsy.2026.107971 [doi] AB - Blockchain can strengthen supply-chain transparency and traceability, but implementation does not necessarily imply full acceptance of the visibility it creates. This study examines how psychological transparency risk, economic transparency risk, and traceability resistance relate to blockchain traceability implementation in Saudi manufacturing firms. Drawing on Transparency Paradox Theory, Information Asymmetry Theory, and Technology Threat Avoidance Theory, the model distinguishes risk appraisals from organisational resistance behaviour. Three-wave survey data from 703 firms were analyzed using PLS-SEM, supported by confirmatory factor analysis, predictive assessment, unit-weighted comparison, and Gaussian Copula analysis. The model explained 67.3% of the variance in implementation and 66.7% in resistance. Contrary to expectations, psychological and economic transparency risks were positively associated with implementation and negatively associated with resistance. Traceability resistance was negatively associated with implementation and was the only direct relationship supported in the hypothesized direction. The indirect effects through resistance were positive, while relational trust and digital capability produced asymmetric moderating effects. The findings show that recognising exposure, control, and commercial vulnerability should not be equated with resistance. Firms may acknowledge these risks while continuing to implement blockchain, whereas delayed integration, restricted access, selective disclosure, and symbolic compliance remain important barriers. The study contributes by separating transparency-risk appraisal, resistance behaviour, and operational implementation, while offering practical guidance on differentiated access controls, disclosure governance, and monitoring of observable resistance. CI - Copyright (c) 2026 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Miralam, Mohammad Saleh AU - Miralam MS AD - Department of Business Administration, College of Business, University of Jeddah, Hamzah Ibn Al Qasim St, Al Sharafeyah, Jeddah, 23218, Makkah Province, Saudi Arabia. Electronic address: mmiralam@uj.edu.sa. FAU - Ahmed, Saif AU - Ahmed S AD - Graduate School of Business, Universiti Kebangsaan Malaysia, Bangi, Malaysia. Electronic address: saifahamed01@gmail.com. FAU - Nazneen, Afroze AU - Nazneen A AD - Department of Business Administration, College of Business, University of Jeddah, Jeddah, Saudi Arabia. Electronic address: anazneen@uj.edu.sa. FAU - Haque, Rasheedul AU - Haque R AD - School of Management & Business (SOMB), MILA University, Nilai, Malaysia. Electronic address: rasheedul.haque@mila.edu.my. FAU - Qazi, Sayeeduzzafar AU - Qazi S AD - College of Business Administration, University of Business and Technology, Jeddah, Saudi Arabia. Electronic address: sayeed@ubt.edu.sa. FAU - Senathirajah, Abdul Rahman Bin S AU - Senathirajah ARBS AD - Faculty of Business and Communications, INTI International University, Malaysia. Electronic address: arahman.senathirajah@newinti.edu.my. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Acta Psychol (Amst) JT - Acta psychologica JID - 0370366 SB - IM OTO - NOTNLM OT - Blockchain adoption OT - Digital capability OT - Economic Growth, Vision 2030 OT - Information asymmetry OT - Psychological and economic risk OT - Relational trust OT - Saudi manufacturing OT - Supply-chain transparency OT - Technology threat avoidance OT - Traceability resistance OT - Transparency paradox COIS- Declaration of competing interest The authors declare no conflict of interest. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:06 PHST- 2026/01/10 00:00 [received] PHST- 2026/07/20 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:06 [entrez] AID - S0001-6918(26)01772-5 [pii] AID - 10.1016/j.actpsy.2026.107971 [doi] PST - aheadofprint SO - Acta Psychol (Amst). 2026 Oct 27;271:107971. doi: 10.1016/j.actpsy.2026.107971. PMID- 42945727 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-2690 (Electronic) IS - 0981-9428 (Linking) VI - 240 DP - 2026 Oct 16 TI - Tartary buckwheat cotyledon size varieties undergo distinct transcriptional reprogramming of phytohormone signaling under cold and UV stresses. PG - 111848 LID - S0981-9428(26)00834-X [pii] LID - 10.1016/j.plaphy.2026.111848 [doi] AB - Fagopyrum tataricum (TB) is a characteristic high-altitude crop in China. The cotyledon, as a key photosynthetic and nutrient storage organ at the seedling stage, plays critical roles in early growth and stress tolerance, but the molecular linkage between cotyledon size and abiotic stress resistance remains unclear. In this study, 279 TB accessions were phenotyped for cotyledon area, height, and width across three ecological regions over two consecutive years, and were classified into large, medium, and small cotyledon types via phenotypic clustering analysis. Two representative accessions, Ft104 (large cotyledon) and Ft32 (small cotyledon), were selected for further experiments. Cytological observations showed that cotyledon size was associated with differences in epidermal cell size and density, with small-cotyledon accessions exhibiting a higher number of cells per unit area. Physiological assays at the germination stage demonstrated that small-cotyledon accessions exhibited faster water absorption, stronger seedling emergence capacity, and more stable degradation of storage substances. Under low-temperature and UV-B stress, small-cotyledon accessions accumulated less malondialdehyde (MDA) and displayed significantly higher activities of peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT), indicating enhanced stress tolerance. Transcriptomic analysis and WGCNA identified 18 co-expression modules, of which MEcoral4 was associated with cotyledon morphology and stress-related physiological traits and was enriched in plant hormone signal transduction. Three candidate genes, FtAUX22D, FtARG7, and FtPYL4, were further examined by qRT-PCR across multiple accessions. Heterologous expression in yeast provided preliminary evidence that FtPYL4 enhances cold-stress tolerance. Targeted LC-MS/MS profiling further revealed genotype-dependent changes in endogenous phytohormones and auxin-related metabolites, with Ft32 showing higher accumulation of several indole-related metabolites and SA under stress, whereas Ft104 exhibited a pronounced JA response to UV-B. Together, these results indicate an association between cotyledon-size phenotype and abiotic-stress responses involving phytohormone-related transcriptional and metabolic reprogramming in Tartary buckwheat. CI - Copyright (c) 2026 The Authors. Published by Elsevier Masson SAS.. All rights reserved. FAU - Liu, Longlong AU - Liu L AD - Houji Lab in Shanxi Province, Shanxi Agricultural University, Taiyuan, Shanxi, 030031, China; Center for Agricultural Genetic Resources Research, Shanxi Agricultural University, Taiyuan, Shanxi, 030031, China. FAU - Sai, Fumeng AU - Sai F AD - College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi, 030801, China. FAU - Shao, Jinghao AU - Shao J AD - College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi, 030801, China. FAU - Zhang, Weiwei AU - Zhang W AD - College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi, 030801, China. FAU - Fan, Jie AU - Fan J AD - College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi, 030801, China. FAU - Ma, MingChuan AU - Ma M AD - Houji Lab in Shanxi Province, Shanxi Agricultural University, Taiyuan, Shanxi, 030031, China; Center for Agricultural Genetic Resources Research, Shanxi Agricultural University, Taiyuan, Shanxi, 030031, China. FAU - Liu, Zhang AU - Liu Z AD - Houji Lab in Shanxi Province, Shanxi Agricultural University, Taiyuan, Shanxi, 030031, China; Center for Agricultural Genetic Resources Research, Shanxi Agricultural University, Taiyuan, Shanxi, 030031, China. FAU - Xu, Shirui AU - Xu S AD - Houji Lab in Shanxi Province, Shanxi Agricultural University, Taiyuan, Shanxi, 030031, China; Center for Agricultural Genetic Resources Research, Shanxi Agricultural University, Taiyuan, Shanxi, 030031, China. FAU - Jiao, Xiongfei AU - Jiao X AD - Shanxi Agricultural University/Shanxi Academy of Agricultural Sciences, Maize Reserch Institute, Xinzhou, Shanxi, 034000, China. FAU - Hou, Siyu AU - Hou S AD - Houji Lab in Shanxi Province, Shanxi Agricultural University, Taiyuan, Shanxi, 030031, China; College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi, 030801, China. Electronic address: siyuhou@sxau.edu.cn. FAU - Sun, Zhaoxia AU - Sun Z AD - Houji Lab in Shanxi Province, Shanxi Agricultural University, Taiyuan, Shanxi, 030031, China; College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi, 030801, China. Electronic address: zhaoxiasun@sxau.edu.cn. LA - eng PT - Journal Article DEP - 20261016 PL - France TA - Plant Physiol Biochem JT - Plant physiology and biochemistry : PPB JID - 9882449 SB - IM OTO - NOTNLM OT - Abiotic stress OT - Cotyledon size OT - Tartary buckwheat OT - WGCNA COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:05 PHST- 2026/04/22 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:05 [entrez] AID - S0981-9428(26)00834-X [pii] AID - 10.1016/j.plaphy.2026.111848 [doi] PST - aheadofprint SO - Plant Physiol Biochem. 2026 Oct 16;240:111848. doi: 10.1016/j.plaphy.2026.111848. PMID- 42945726 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-2690 (Electronic) IS - 0981-9428 (Linking) VI - 240 DP - 2026 Oct 22 TI - Binding pocket optimization of Pueraria mirifica type II chalcone isomerase synergizing with a necrosis-attenuating host transcription factor for persistent daidzein biosynthesis. PG - 111883 LID - S0981-9428(26)00869-7 [pii] LID - 10.1016/j.plaphy.2026.111883 [doi] AB - Pathway reconstitution of leguminous isoflavonoids in non-legume plant chassis offers a tractably controlled environment to probe specialized metabolism, yet it remains critically bottlenecked by suboptimal heterologous enzyme efficiency and severe host tissue necrosis. Here, we overcome these long-standing architectural limitations by establishing a highly synergistic, necrosis-attenuated Nicotiana benthamiana synthetic biology platform. We characterized chalcone isomerases from Pueraria mirifica (PmCHIs), revealing that PmCHI Type II operates as a biochemical junction exhibiting a specialized substrate-dependent alkaline optimum (pH 7.5-8.0) exclusively toward isoliquiritigenin. Combining 500-ns molecular dynamics simulations with structure-guided mutagenesis, active-site engineering identified an I195V variant with enhanced isoliquiritigenin preference. The conservative I195V substitution was predicted to reduce local steric crowding and produced an isoliquiritigenin selectivity ratio of 1.63 in vitro. For in planta pathway integration, we delivered a polycistronic cascade using P2A ribosome-skipping peptides to streamline multiplex multi-gene stacking. Crucially, substituting the conventional Arabidopsis thaliana MYB12 regulator with a host-derived N. benthamiana MYB60 transcription factor successfully attenuated tissue necrosis, accompanied by reduced electrolyte leakage. Quantitative proteomics showed no statistically significant difference in PmCHI abundance between the I195V and WT-II treatments in planta, while independent plant batches consistently showed higher daidzein accumulation with I195V, although the magnitude of enhancement varied among experiments. The NbMYB60-based platform extended the operational viability window up to 9 days, delivering a robust daidzein titer of 1.5 mg/g DW at 6 days post-infiltration, with individual high-performing replicates reaching up to 2.5 mg/g DW. Together, these results establish an integrated enzyme-engineering and necrosis-attenuating regulatory framework for persistent daidzein biosynthesis and specialized pathway reconstitution in planta. CI - Copyright (c) 2026 The Authors. Published by Elsevier Masson SAS.. All rights reserved. FAU - Suntichaikamolkul, Nithiwat AU - Suntichaikamolkul N AD - Department of Biochemistry, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand; Center of Excellence in Molecular Crop, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand. FAU - Sanachai, Kamonpan AU - Sanachai K AD - Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand. FAU - Nutho, Bodee AU - Nutho B AD - Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand. FAU - Rungrotmongkol, Thanyada AU - Rungrotmongkol T AD - Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand; Center of Excellence in Structural and Computational Biology, Chulalongkorn University, Bangkok, 10330, Thailand; Program in Bioinformatics and Computational Biology, College of Interdisciplinary and Integrative Studies, Chulalongkorn University, Bangkok, 10330, Thailand. FAU - Tubtimrattana, Apinya AU - Tubtimrattana A AD - Department of Forensic Medicine, King Chulalongkorn Memorial Hospital, The Thai Red Cross Society, Bangkok, 10330, Thailand. FAU - Thirati, Parath AU - Thirati P AD - Department of Forensic Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand. FAU - Sucharitakul, Jeerus AU - Sucharitakul J AD - Department of Biochemistry, Faculty of Dentistry, Chulalongkorn University, Bangkok, 10330, Thailand. Electronic address: jeerus.s@chula.ac.th. FAU - Sirikantaramas, Supaart AU - Sirikantaramas S AD - Center of Excellence in Molecular Crop, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand. Electronic address: supaart.s@chula.ac.th. LA - eng PT - Journal Article DEP - 20261022 PL - France TA - Plant Physiol Biochem JT - Plant physiology and biochemistry : PPB JID - 9882449 SB - IM OTO - NOTNLM OT - Active-site engineering OT - Chalcone isomerase OT - Daidzein biosynthesis OT - NbMYB60 OT - Nicotiana benthamiana OT - Pueraria mirifica COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:05 PHST- 2026/07/27 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:05 [entrez] AID - S0981-9428(26)00869-7 [pii] AID - 10.1016/j.plaphy.2026.111883 [doi] PST - aheadofprint SO - Plant Physiol Biochem. 2026 Oct 22;240:111883. doi: 10.1016/j.plaphy.2026.111883. PMID- 42945725 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-2690 (Electronic) IS - 0981-9428 (Linking) VI - 240 DP - 2026 Oct 8 TI - Genome-wide identification of the CPA family in Ipomoea pes-caprae reveals their roles in salt stress tolerance and ion homeostasis regulation. PG - 111838 LID - S0981-9428(26)00824-7 [pii] LID - 10.1016/j.plaphy.2026.111838 [doi] AB - High soil salinity is a major environmental constraint that limits global agricultural productivity and plant development. The current research aimed to perform a comprehensive assessment of the cation/proton antiporter (CPA) gene family in the highly adaptable coastal halophyte beach morning glory (Ipomoea pes-caprae (L.) R. Br.). Thirty-two CPA genes were identified across 15 chromosomes and categorized into three distinct subfamilies: sodium/hydrogen exchanger (NHX), potassium efflux antiporter (KEA), and cation/hydrogen exchanger (CHX). To characterize the regulatory architecture and transcriptional dynamics during high-salinity exposure, promoter evaluations and quantitative real-time polymerase chain reaction (RT-qPCR) analyses were utilized. Computational scanning of upstream regulatory sequences revealed a high abundance of cis-acting motifs linked to environmental adversity and phytohormone signaling in CPA gene promoters. Transcriptomic and RT-qPCR expression profiles further demonstrated that the CPA genes display organ-specific and temporally dynamic expression under high-salinity conditions. Functional validation was performed through heterologous overexpression of selected genes (IpcNHX2, IpcKEA6, IpcCHX3, and IpcCHX5) in Arabidopsis, and this overexpression significantly enhanced the salt tolerance of transformed lines during extreme saline treatments (up to 150 mM NaCl). Physiological analyses further demonstrated that IpcCPA overexpression improved biomass maintenance and promoted ionic homeostasis by reducing Na (+) accumulation, maintaining higher K(+) levels, and increasing the K(+)/Na(+) ratio under salt stress. Furthermore, yeast complementation assays revealed that these CPA proteins partially restored the growth defect of the potassium-deficient CY162 mutant for growth defect of the potassium-deficient CY162 mutant, suggesting their potential involvement in potassium homeostasis. In summary, this study provides a comprehensive genomic evaluation of the CPA family in I. pes-caprae, highlights the potential roles of CPA members in salt stress adaptation, and offers valuable candidate genes for crop improvement. CI - Copyright (c) 2026. Published by Elsevier Masson SAS. FAU - Lin, Xinqiang AU - Lin X AD - College of Life Sciences, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, School of Future Technology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou, 350108, China. FAU - Qu, Tong AU - Qu T AD - College of Life Sciences, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, School of Future Technology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Chen, Xingguang AU - Chen X AD - College of Life Sciences, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, School of Future Technology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Li, Xueli AU - Li X AD - College of Life Sciences, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, School of Future Technology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Wang, Xianfeng AU - Wang X AD - College of Life Sciences, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, School of Future Technology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou, 350108, China. FAU - Zhang, Mingyue AU - Zhang M AD - College of Life Sciences, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, School of Future Technology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Li, Xinyu AU - Li X AD - College of Life Sciences, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, School of Future Technology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Wu, Ting AU - Wu T AD - College of Life Sciences, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, School of Future Technology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Si, Shuo AU - Si S AD - College of Life Sciences, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, School of Future Technology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Feng, Haoran AU - Feng H AD - College of Life Sciences, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, School of Future Technology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Hu, Jinbin AU - Hu J AD - College of Life Sciences, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, School of Future Technology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Cai, Jiahao AU - Cai J AD - College of Life Sciences, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, School of Future Technology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Yang, Shuqi AU - Yang S AD - College of Life Sciences, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, School of Future Technology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Pan, Chenglang AU - Pan C AD - Fujian Key Laboratory on Conservation and Sustainable Utilization of Marine Biodiversity, Fuzhou Institute of Oceanography, Minjiang University, Fuzhou, 350108, China. FAU - Wang, Lulu AU - Wang L AD - College of Life Sciences, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, School of Future Technology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Niu, Xiaoping AU - Niu X AD - College of Life Sciences, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, School of Future Technology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. FAU - Qin, Yuan AU - Qin Y AD - College of Life Sciences, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, School of Future Technology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address: yuanqin001@foxmail.com. FAU - Cheng, Yan AU - Cheng Y AD - College of Life Sciences, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, School of Future Technology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address: chengyan1220@hotmail.com. LA - eng PT - Journal Article DEP - 20261008 PL - France TA - Plant Physiol Biochem JT - Plant physiology and biochemistry : PPB JID - 9882449 SB - IM OTO - NOTNLM OT - Cation/proton antiporter OT - Ipomoea pes-Caprae OT - Na(+)/K(+) homeostasis OT - Salinity tolerance COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:05 PHST- 2026/04/15 00:00 [received] PHST- 2026/08/22 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:05 [entrez] AID - S0981-9428(26)00824-7 [pii] AID - 10.1016/j.plaphy.2026.111838 [doi] PST - aheadofprint SO - Plant Physiol Biochem. 2026 Oct 8;240:111838. doi: 10.1016/j.plaphy.2026.111838. PMID- 42945716 OWN - NLM STAT- Publisher LR - 20261027 IS - 1090-2120 (Electronic) IS - 0045-2068 (Linking) VI - 184 DP - 2026 Oct 16 TI - JDW312 enhances gemcitabine efficacy in pancreatic cancer through ROS/MEK/ERK activation. PG - 110656 LID - S0045-2068(26)01192-2 [pii] LID - 10.1016/j.bioorg.2026.110656 [doi] AB - Pancreatic cancer is a highly aggressive malignancy with late diagnosis, early metastasis, poor prognosis, and limited therapeutic options. To address this unmet clinical need, we employed JOA as a lead compound and developed its derivative, JDW312, through structural optimization. The effects and underlying mechanisms of JDW312 were investigated using cell proliferation assays, flow cytometry, Western blotting, immunohistochemistry, and SW1990 xenograft models. In vitro, JDW312 exhibited potent and moderately selective anti-proliferative activity against pancreatic cancer cells SW1990 (IC(50) = 0.93 +/- 0.08 muM), primarily through activation of the ROS/MEK/ERK signaling pathway, leading to apoptosis and autophagy induction and suppression of cell proliferation. In vivo, JDW312 (20 mg/kg, tumor inhibition rate 53.04%) showed significantly greater anti-tumor efficacy than oridonin (20 mg/kg, tumor inhibition rate 25.16%). Moreover, its combination with gemcitabine markedly enhanced anti-tumor activity while maintaining a tolerable safety profile. Collectively, these findings identify JDW312 as a promising therapeutic candidate for pancreatic cancer, either alone or in combination with standard chemotherapy. CI - Copyright (c) 2026. Published by Elsevier Inc. FAU - Wang, Wang AU - Wang W AD - Luoyang Key Laboratory of Organic Functional Molecules, School of Food and Drug, Luoyang Normal University, Luoyang, 471934, China; Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, Zhengzhou, 450003, China. FAU - Ding, Xinfang AU - Ding X AD - School of Pharmaceutical Sciences; Institute of Drug Discovery and Development; Key Laboratory of Advanced Pharmaceutical Technology, Ministry of Education of China, Zhengzhou University, Zhengzhou, 450001, China. FAU - Liu, Haibin AU - Liu H AD - School of Pharmaceutical Sciences; Institute of Drug Discovery and Development; Key Laboratory of Advanced Pharmaceutical Technology, Ministry of Education of China, Zhengzhou University, Zhengzhou, 450001, China. FAU - Miao, Yanan AU - Miao Y AD - School of Pharmaceutical Sciences; Institute of Drug Discovery and Development; Key Laboratory of Advanced Pharmaceutical Technology, Ministry of Education of China, Zhengzhou University, Zhengzhou, 450001, China. FAU - Geng, Mingge AU - Geng M AD - School of Pharmaceutical Sciences; Institute of Drug Discovery and Development; Key Laboratory of Advanced Pharmaceutical Technology, Ministry of Education of China, Zhengzhou University, Zhengzhou, 450001, China. FAU - Huang, Qile AU - Huang Q AD - School of Pharmaceutical Sciences; Institute of Drug Discovery and Development; Key Laboratory of Advanced Pharmaceutical Technology, Ministry of Education of China, Zhengzhou University, Zhengzhou, 450001, China. FAU - Cui, Yafei AU - Cui Y AD - School of Pharmaceutical Sciences; Institute of Drug Discovery and Development; Key Laboratory of Advanced Pharmaceutical Technology, Ministry of Education of China, Zhengzhou University, Zhengzhou, 450001, China. FAU - Gao, Feixiang AU - Gao F AD - School of Pharmaceutical Sciences; Institute of Drug Discovery and Development; Key Laboratory of Advanced Pharmaceutical Technology, Ministry of Education of China, Zhengzhou University, Zhengzhou, 450001, China. FAU - Guo, Yue AU - Guo Y AD - Luoyang Key Laboratory of Organic Functional Molecules, School of Food and Drug, Luoyang Normal University, Luoyang, 471934, China. FAU - Jing, Shuo AU - Jing S AD - Luoyang Key Laboratory of Organic Functional Molecules, School of Food and Drug, Luoyang Normal University, Luoyang, 471934, China. FAU - Xie, Hang AU - Xie H AD - Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China. Electronic address: 20111030063@fudan.edu.cn. FAU - Liu, Hongmin AU - Liu H AD - School of Pharmaceutical Sciences; Institute of Drug Discovery and Development; Key Laboratory of Advanced Pharmaceutical Technology, Ministry of Education of China, Zhengzhou University, Zhengzhou, 450001, China. Electronic address: liuhm@zzu.edu.cn. FAU - Ke, Yu AU - Ke Y AD - School of Pharmaceutical Sciences; Institute of Drug Discovery and Development; Key Laboratory of Advanced Pharmaceutical Technology, Ministry of Education of China, Zhengzhou University, Zhengzhou, 450001, China. Electronic address: ky@zzu.edu.cn. LA - eng PT - Journal Article DEP - 20261016 PL - United States TA - Bioorg Chem JT - Bioorganic chemistry JID - 1303703 SB - IM OTO - NOTNLM OT - Ent-kaurene diterpenoid OT - Gemcitabine OT - Pancreatic cancer OT - ROS COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:05 PHST- 2026/08/20 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:05 [entrez] AID - S0045-2068(26)01192-2 [pii] AID - 10.1016/j.bioorg.2026.110656 [doi] PST - aheadofprint SO - Bioorg Chem. 2026 Oct 16;184:110656. doi: 10.1016/j.bioorg.2026.110656. PMID- 42945714 OWN - NLM STAT- Publisher LR - 20261027 IS - 1090-2120 (Electronic) IS - 0045-2068 (Linking) VI - 184 DP - 2026 Oct 26 TI - Development of a near-infrared two-photon fluorescent probe for highly sensitivity cysteine detection in Parkinson's disease via an electronic regulation strategy. PG - 110688 LID - S0045-2068(26)01224-1 [pii] LID - 10.1016/j.bioorg.2026.110688 [doi] AB - Parkinson's disease is a prevalent neurodegenerative disorder strongly linked to dysregulation of cysteine (Cys) metabolism. Real-time and highly sensitive monitoring of Cys levels in PD pathological microenvironments is crucial for revealing the pathogenesis and realizing early diagnosis of PD. This study highlights a valid strategy for rapidly constructing a novel Cys probe called (E)-4-(2-(3-(dicyanomethylene)-5,5-dimethylcyclohex-1-en-1-yl)vinyl)-2-fluorophenyl acrylate (DMVF) by accelerating the reaction rate with Cys compare with (E)-2-chloro-4-(2-(3-(dicyanomethylene)-5,5-dimethylcyclohex-1-en-1-yl)vinyl)phenyl acrylate (DMVCl) via an electronic regulation strategy. Specifically, through merely altering fluorine atom in the fluorophore FHD-OH, its pKa is thereby decreased to facilitate its proton dissociation under physiological conditions. DMV-F not only exhibits a ultra-high sensitivity (detection limit, LOD = 6.97 nM), rapid response (within 55 s), and nice selectivity for Cys over other biological interferents. DMV-F demonstrates NIR emission at 680 nm with two-photon excitation at 538 nm. DMV-F can visualize endogenous and exogenous Cys fluctuations in HeLa cells. More importantly, DMV-F successfully achieves real-time two-photon fluorescence imaging of Cys in a cellular model of PD disease model. This work provides a powerful optical tool for in-situ and dynamic monitoring of Cys in PD research, and offers a new design idea for electronic regulation-based construction of high-performance fluorescent probes for neurodegenerative diseases. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Du, Yuting AU - Du Y AD - Department of Chemistry, Xinzhou Normal University, Xinzhou, Shanxi, 034000, China. Electronic address: yutingdu123@163.com. FAU - Wang, Hongliang AU - Wang H AD - Department of Chemistry, Xinzhou Normal University, Xinzhou, Shanxi, 034000, China. FAU - Guo, Minmin AU - Guo M AD - Department of Chemistry, Xinzhou Normal University, Xinzhou, Shanxi, 034000, China. FAU - Zhao, Sanhu AU - Zhao S AD - Department of Chemistry, Xinzhou Normal University, Xinzhou, Shanxi, 034000, China. FAU - Ren, Haixian AU - Ren H AD - Department of Chemistry, Xinzhou Normal University, Xinzhou, Shanxi, 034000, China. FAU - Zhang, Ting AU - Zhang T AD - Department of Chemistry, Xinzhou Normal University, Xinzhou, Shanxi, 034000, China. LA - eng PT - Journal Article DEP - 20261026 PL - United States TA - Bioorg Chem JT - Bioorganic chemistry JID - 1303703 SB - IM OTO - NOTNLM OT - Cells bioimaging OT - Cysteine OT - Near-infrared two-photon fluorescent probe OT - Parkinson's disease COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:05 PHST- 2026/08/04 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:05 [entrez] AID - S0045-2068(26)01224-1 [pii] AID - 10.1016/j.bioorg.2026.110688 [doi] PST - aheadofprint SO - Bioorg Chem. 2026 Oct 26;184:110688. doi: 10.1016/j.bioorg.2026.110688. PMID- 42945700 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-2518 (Electronic) IS - 0264-410X (Linking) VI - 95 DP - 2026 Oct 27 TI - Childhood vaccination timeliness, under-immunization-for-age and measles effective immunity in Peru: a province and birth-cohort analysis of national survey data, 2004-2025. PG - 129286 LID - S0264-410X(26)01095-9 [pii] LID - 10.1016/j.vaccine.2026.129286 [doi] AB - BACKGROUND: Using the survey rounds 2004-2025 of Peru's Demographic and Family Health Survey (ENDES) for children aged 12-35 months, we estimated the timeliness of sentinel vaccine doses; under-immunization-for-age and late receipt by province and birth cohort; and the gap between the proportion of children effectively immune to measles and the herd-immunity threshold. METHODS: Pooled cross-sections were analysed. Timeliness was measured in days, within World Health Organization age bands, among children whose vaccination card was seen; weighting and bounds addressed selection. We estimated the share of the maternal-education gap in timeliness running through antenatal and delivery care and fitted Bayesian small-area models for 196 provinces. RESULTS: Among card-seen children, on-time receipt was 86.8% for bacille Calmette-Guerin (BCG), 65.3% for the third dose of diphtheria-tetanus-pertussis (DTP)-containing vaccine (DTP3), 61.1% for the first measles-containing dose and, in survey rounds 2015-2025, 39.2% for the second. For all children in the age band, recall-based bounds put DTP3 timeliness at 54.5%-63.5%, below the card-seen estimate. Children of more educated mothers had a 6.7-point lower risk of DTP3 not on time; under stated assumptions, antenatal care and institutional delivery explained 0.85 points (13%) of this gap. In the 2020-2025 birth cohort, 28 provinces were classed worse than the national level for DTP3 not on time. In the 2020-2024 birth cohort, the province-average proportion of children effectively immune to measles was 89.8%, below the herd-immunity thresholds for a basic reproduction number (R0) of 15-18; for R0 = 12, the average probability that a province fell below the threshold was 59.9%. CONCLUSIONS: Many card-seen children were vaccinated late, and card-based estimates probably overstate DTP3 timeliness. Under the assumed vaccine effectiveness and R0 of 15-18, measles immunity stayed below herd-immunity thresholds. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Rojas, Jesus D AU - Rojas JD AD - Universidad Privada Norbert Wiener, Lima, 1506, Peru. Electronic address: jesus.rojas@uwiener.edu.pe. FAU - Huarachi-Napa, Carolina R AU - Huarachi-Napa CR AD - Universidad Privada Norbert Wiener, Lima, 1506, Peru. FAU - Salazar-Buitron, Stephanie Z AU - Salazar-Buitron SZ AD - Universidad Privada Norbert Wiener, Lima, 1506, Peru. FAU - Quillo-Diaz, Angelo P AU - Quillo-Diaz AP AD - Universidad Privada Norbert Wiener, Lima, 1506, Peru. FAU - Calderon, Roger I AU - Calderon RI AD - Universidad Privada Norbert Wiener, Lima, 1506, Peru. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Vaccine JT - Vaccine JID - 8406899 SB - IM OTO - NOTNLM OT - Health surveys OT - Immunization schedule OT - Measles OT - Peru OT - Small-area analysis OT - Vaccination timeliness COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:04 PHST- 2026/07/30 00:00 [received] PHST- 2026/10/21 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:04 [entrez] AID - S0264-410X(26)01095-9 [pii] AID - 10.1016/j.vaccine.2026.129286 [doi] PST - aheadofprint SO - Vaccine. 2026 Oct 27;95:129286. doi: 10.1016/j.vaccine.2026.129286. PMID- 42945692 OWN - NLM STAT- Publisher LR - 20261027 IS - 1361-648X (Electronic) IS - 0953-8984 (Linking) DP - 2026 Oct 27 TI - Local moment magnon spectrum in conduction electron tunnelling. LID - 10.1088/1361-648X/aeba2d [doi] AB - The surface tunnelling spectrum of a dual system consisting of localised moments with antiferromagnetic order coupled to conduction electrons by on-site exchange interaction is investigated. In the static approximation of the local moment order it is known that magnetic band reconstruction leads to an anomalous tunnelling spectrum at the magnetic ordering vector of local moments although the latter cannot contribute directly. In this work we consider dynamic effects by including the scattering of conduction electrons from the local moment magnon excitations. They lead to self energies and renormalisation of conduction states which in turn appreciably modify the tunnelling spectrum beyond the influence of static order, interpreted as the appearance of magnon sidebands. CI - (c) 2026 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved. FAU - Thalmeier, Peter AU - Thalmeier P AUID- ORCID: 0000-0003-2220-0401 AD - Nothnitzer Str. 40, Max-Planck-Institute for Chemical Physics of Solids, D-01187 Dresden,, Dresden, SN, 01187, Germany. FAU - Akbari, Alireza AU - Akbari A AUID- ORCID: 0000-0002-6234-0575 AD - Yanqi Lake Beijing Institute of Mathematical Sciences and Applications, Yanqihu, Huairou District,, Beijing, None Selected, 101408, China. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - J Phys Condens Matter JT - Journal of physics. Condensed matter : an Institute of Physics journal JID - 101165248 SB - IM OTO - NOTNLM OT - antiferromagnetic order OT - conduction electron tunnelling OT - electron-magnon interaction OT - local moment magnetism OT - magnon spectrum OT - quasiparticle self-energy OT - scanning tunnelling microscopy EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:04 PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:04 [entrez] AID - 10.1088/1361-648X/aeba2d [doi] PST - aheadofprint SO - J Phys Condens Matter. 2026 Oct 27. doi: 10.1088/1361-648X/aeba2d. PMID- 42945688 OWN - NLM STAT- Publisher LR - 20261027 IS - 1540-1413 (Electronic) IS - 1540-1405 (Linking) DP - 2026 Oct 27 TI - New and Emerging Options for the Treatment of Non-Muscle-Invasive Bladder Cancer. LID - e267061 [pii] LID - 10.6004/jnccn.2026.7061 [doi] AB - Risk-adapted management of non-muscle-invasive bladder cancer (NMIBC) ranges from surveillance for low-risk tumors to intravesical chemotherapy or bacillus Calmette-Guerin (BCG) for intermediate- and high-risk disease. Radical cystectomy is reserved for patients experiencing cancer recurrence or progression despite treatment and for those with very high-risk features. The landscape of management options is rapidly evolving, with several new agents recently approved for BCG-unresponsive disease. In addition, many clinical trials are ongoing across the spectrum of NMIBC disease states. This review aims to summarize new and emerging treatment options in NMIBC. FAU - Nusbaum, David J AU - Nusbaum DJ AD - 1Department of Genitourinary Oncology, Moffitt Cancer Center, Tampa, FL. FAU - Ferguson, James E 3rd AU - Ferguson JE 3rd AD - 2Department of Urology, University of Alabama at Birmingham, Birmingham, AL. FAU - Spiess, Philippe E AU - Spiess PE AD - 1Department of Genitourinary Oncology, Moffitt Cancer Center, Tampa, FL. FAU - Li, Roger AU - Li R AD - 1Department of Genitourinary Oncology, Moffitt Cancer Center, Tampa, FL. FAU - Fang, Andrew M AU - Fang AM AD - 2Department of Urology, University of Alabama at Birmingham, Birmingham, AL. FAU - Sundi, Debasish AU - Sundi D AD - 3Department of Urology, Ohio State University, Columbus, OH. FAU - Gilbert, Scott M AU - Gilbert SM AD - 1Department of Genitourinary Oncology, Moffitt Cancer Center, Tampa, FL. FAU - Peyton, Charles C AU - Peyton CC AD - 2Department of Urology, University of Alabama at Birmingham, Birmingham, AL. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - United States TA - J Natl Compr Canc Netw JT - Journal of the National Comprehensive Cancer Network : JNCCN JID - 101162515 SB - IM OTO - NOTNLM OT - BCG-unresponsive OT - Nonmuscle invasive bladder cancer OT - Urothelial carcinoma OT - intravesical therapy EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:04 PHST- 2026/03/16 00:00 [received] PHST- 2026/06/24 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:04 [entrez] AID - e267061 [pii] AID - 10.6004/jnccn.2026.7061 [doi] PST - aheadofprint SO - J Natl Compr Canc Netw. 2026 Oct 27:e267061. doi: 10.6004/jnccn.2026.7061. PMID- 42945685 OWN - NLM STAT- Publisher LR - 20261027 IS - 1758-5090 (Electronic) IS - 1758-5082 (Linking) DP - 2026 Oct 27 TI - Engineering the synovial lining-sublining architecture in a human 3D model to study macrophage-fibroblast interactions in arthritis. LID - 10.1088/1758-5090/aeba1a [doi] AB - The synovial membrane is a key regulator of joint homeostasis, inflammation, and tissue remodeling, yet human in vitro models that reproduce its spatial organization while enabling controlled investigation of macrophage-fibroblast-like stromal cell (FLS) interactions remain limited. Here, we developed a xeno-free, spatially organized 3D synovial membrane model that reconstructs the structural relationship between the macrophage lining and fibroblast-rich sublining compartment. The model was generated by establishing an apically organized macrophage-containing lining-like compartment on a transwell membrane, followed by stepwise assembly of FLS laden hydrogel constructs. Hydrogel screening identified a 1:3 VitroGel(R) RGD formulation as the most suitable condition to support FLS viability, homogeneous cell distribution, and stable tissue organization. Experimentally induced M1-like and M2-like macrophages were incorporated, and the model was stimulated with TNF-alpha, TGF-beta, or OA synovial fluid (SF). Cytotoxicity, proliferation, metabolic activity, gene expression, and cytokine secretion were analyzed. Pharmacological responsiveness was evaluated using adalimumab and 4-octyl itaconate (4-OI). The spatially organized construct enabled compartment-specific analysis of macrophage-FLS communication. M1-like macrophages induced inflammatory FLS responses characterized by elevated IL1B, IL6, TNFA, and MMP1 expression, whereas M2-like macrophages promoted matrix-remodeling and myofibroblast-related programs, including increased TGFB and ACTA2 expression. Exposure to OA-SF and cytokine stimulation induced FLS hyperproliferation, metabolic adaptation, and distinct transcriptional responses. Treatment with adalimumab suppressed TNF-driven inflammatory responses, whereas 4-OI reduced selected inflammatory markers and altered remodeling-associated responses. Together, these findings establish a spatially organized, xeno-free 3D synovial membrane model as a defined and experimentally tractable platform for studying macrophage-FLS communication and synovial activation. By combining physiologically inspired tissue organization with compartment-specific analysis, the model provides a human in vitro platform for investigating OA-associated inflammatory and remodeling processes and evaluating pharmacological modulation under defined experimental conditions. CI - Creative Commons Attribution license. FAU - Gaber, Timo AU - Gaber T AD - Charite Universitatsmedizin Berlin, Chariteplatz 1, Berlin, 10117, Germany. FAU - Pfeiffenberger, Moritz AU - Pfeiffenberger M AD - Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Chariteplatz 1, Berlin, 10117, Germany. FAU - Beissel, Julia AU - Beissel J AD - Charite -Universitatsmedizin Berlin, Chariteplatz 1, Berlin, 10117, Germany. FAU - Akil, Nour AU - Akil N AD - Charite -Universitatsmedizin Berlin, Chariteplatz 1, Berlin, 10117, Germany. FAU - Walter-Vogiadji, Josephine AU - Walter-Vogiadji J AUID- ORCID: 0009-0002-6107-6204 AD - Charite -Universitatsmedizin Berlin, Chariteplatz 1, Berlin, 10117, Germany. FAU - Jaehger, Ditte AU - Jaehger D AUID- ORCID: 0000-0003-1782-8157 AD - Department of Health Technology, Technical University of Denmark, Orsteds Plads, Building 345C, Lyngby, 2800, Denmark. FAU - Maleitzke, Tazio AU - Maleitzke T AD - Charite -Universitatsmedizin Berlin, Chariteplatz 1, Berlin, 10117, Germany. FAU - Winkler, Tobias AU - Winkler T AD - Charite -Universitatsmedizin Berlin, Chariteplatz 1, Berlin, 10117, Germany. FAU - Hoff, Paula AU - Hoff P AD - Charite -Universitatsmedizin Berlin, Chariteplatz 1, Berlin, 10117, Germany. FAU - Wiebe, Edgar AU - Wiebe E AD - Charite -Universitatsmedizin Berlin, Chariteplatz 1, Berlin, 10117, Germany. FAU - Buttgereit, Frank AU - Buttgereit F AUID- ORCID: 0000-0003-2534-550X AD - Rheumatology and Clinical Immunology, Charite Universitatsmedizin Berlin, Chariteplatz 1, Berlin, 10117, Germany. FAU - Damerau, Alexandra AU - Damerau A AUID- ORCID: 0000-0001-6651-2963 AD - Charite -Universitatsmedizin Berlin, Chariteplatz 1, Berlin, 10117, Germany. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Biofabrication JT - Biofabrication JID - 101521964 SB - IM OTO - NOTNLM OT - fibrosis OT - immune-stromal interactions OT - organoids OT - synovial membrane model OT - synovitis EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:04 PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:04 [entrez] AID - 10.1088/1758-5090/aeba1a [doi] PST - aheadofprint SO - Biofabrication. 2026 Oct 27. doi: 10.1088/1758-5090/aeba1a. PMID- 42945683 OWN - NLM STAT- Publisher LR - 20261027 IS - 1361-648X (Electronic) IS - 0953-8984 (Linking) DP - 2026 Oct 27 TI - High-pressure regulation effects on the magnetotransport properties of flux-grown ZrTe5 single crystals. LID - 10.1088/1361-648X/aeba30 [doi] AB - Flux-grown ZrTe5 crystals with near-ideal stoichiometric composition exhibit semiconducting behavior at ambient pressure, which can be systematically manipulated by altering the Te content or by applying external pressure or magnetic field. In this study, we investigate the electrical resistivity and magnetoresistivity (MR) of flux-grown ZrTe5 single crystals under pressures up to 7 GPa using a palm cubic anvil high-pressure cell. The pressure-dependent electrical resistivity reveals a systematic crossover from a semiconducting to a semimetallic phase between 3 and 4 GPa, which also coincides with the sign reversal of the Hall resistivity at low temperatures. These experimental observations collectively suggest the occurrence of a pressure-induced Lifshitz-like transition in the 3-4 GPa pressure range. Furthermore, at 7 GPa, we observe a sudden drop in electrical resistivity near 3.4 K, indicative of a superconducting transition. Notably, the observed superconducting transition temperature is nearly twice that reported for previously studied chemical vapor transport (CVT)-grown ZrTe5 crystals (Tc approximately 1.8 K). To gain additional theoretical insight, we also performed first-principles calculations including spin-orbit coupling over a wide 0-6 GPa pressure range. The calculations reveal a substantial reconstruction of the low-energy electronic states, characterized by a pronounced enhancement of the density of states at the Fermi level and a significant modification of the band gap near 3 GPa, consistent with the experimentally observed phenomena. Symmetry-indicator and Wilson-loop analyses were further performed to investigate the evolution of the topological electronic structure under pressure. These findings demonstrate that pressure, together with subtle variations in crystal stoichiometry, drives remarkable electronic and structural transformations in ZrTe5, consistent with the pressure-induced Lifshitz transition and enhanced superconductivity in this Dirac semimetal. CI - (c) 2026 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved. FAU - Mishra, Sanskar AU - Mishra S AD - Department of Physics, Deen Dayal Upadhyaya Gorakhpur University, Civil Lines, Gorakhpur, Gorakhpur, Uttar Pradesh, 273009, India. FAU - Singh, Nagendra AU - Singh N AD - Department of Physics, Deen Dayal Upadhyaya Gorakhpur University, Civil Lines, Gorakhpur, Gorakhpur, 273009, India. FAU - Gangwar, Vinod Kumar AU - Gangwar VK AD - Department of Physics, K.G.K. (P.G.) College, Moradabad, Moradabad, Moradabad, Uttar Pradesh, 244001, India. FAU - Sun, Jianping AU - Sun J AUID- ORCID: 0000-0002-2873-6023 AD - Institute of Physics Chinese Academy of Sciences, Beijing, Beijing, 100190, China. FAU - Uwatoko, Yoshiya AU - Uwatoko Y AUID- ORCID: 0000-0002-1148-4632 AD - Institute for Solid State Physics, The University of Tokyo, Kashiwanoha 5-1-5, Kashiwa, Chiba 277-8581, Chiba, 277-8581, Japan. FAU - Chatterjee, Sandip AU - Chatterjee S AUID- ORCID: 0000-0002-6392-0598 AD - Department of Physics, IIT (BHU), Indian Institute of Technology, Varanasi - 221005, Varanasi, 221005, India. FAU - Cheng, Jinguang AU - Cheng J AUID- ORCID: 0000-0002-4969-1960 AD - Institute of Physics Chinese Academy of Sciences, Beijing, Beijing, 100190, China. FAU - Shahi, Prashant AU - Shahi P AD - Department of Physics, Deen Dayal Upadhyaya Gorakhpur University, Civil Lines, Gorakhpur, Gorakhpur, None Selected, 273009, India. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - J Phys Condens Matter JT - Journal of physics. Condensed matter : an Institute of Physics journal JID - 101165248 SB - IM OTO - NOTNLM OT - Dirac Semimetals OT - High-pressure OT - Lifshitz transition OT - Topological Materials EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:04 PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:04 [entrez] AID - 10.1088/1361-648X/aeba30 [doi] PST - aheadofprint SO - J Phys Condens Matter. 2026 Oct 27. doi: 10.1088/1361-648X/aeba30. PMID- 42945676 OWN - NLM STAT- Publisher LR - 20261027 IS - 1095-7103 (Electronic) IS - 0021-9797 (Linking) VI - 729 DP - 2026 Oct 23 TI - Corrosion-driven interfacial reconstruction enables selective two-carbon ethanol oxidation at industrial current density. PG - 141849 LID - S0021-9797(26)02026-6 [pii] LID - 10.1016/j.jcis.2026.141849 [doi] AB - The sluggish kinetics of the anodic oxygen evolution reaction (OER) severely limit the energy efficiency of conventional water electrolysis. Replacing OER with thermodynamically favorable small-molecule oxidation offers a promising strategy to reduce energy consumption while enabling value-added chemical production. Here, we report a corrosion-driven interfacial reconstruction strategy to in situ construct a self-supported nickel-iron layered double hydroxide/iron oxyhydroxide (NiFe-LDH/FeOOH) heterostructure on iron foam for efficient ethanol electrooxidation (EOR). The hierarchically assembled nanosheet architecture, coupled with a strongly coupled heterointerface, enables abundant accessible active sites and promotes interfacial charge redistribution. As a result, the catalyst delivers a high current density exceeding 400 mA (-2) at 1.5 V along with a high Faradaic efficiency of 92.21% over 3 days, underscoring great potential for EOR at industrial current density. Operando spectroscopic analyses reveal a selective C2 oxidation pathway via acetaldehyde intermediates. Density functional theory (DFT) calculations further demonstrate that interfacial electronic coupling lowers the energy barrier of the rate-determining CH(3)CH(2)O to CH(3)CHO step. This work highlights the critical role of heterointerface engineering in regulating both electronic structure and reaction pathways, providing a viable strategy for coupling energy-efficient hydrogen production with selective biomass upgrading. CI - Copyright (c) 2026 Elsevier Inc. All rights reserved. FAU - Cheng, Siwei AU - Cheng S AD - Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming, 650093, China. FAU - Wang, Junli AU - Wang J AD - Researcher center for analysis and measurement, Kunming University of Science and Technology, Kunming, 650093, PR China. FAU - Yu, Xiancai AU - Yu X AD - Anhui Jidian New Energy Co., Ltd, Hefei, 230000, PR China. FAU - Wang, Yunpeng AU - Wang Y AD - Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming, 650093, China. FAU - Li, Nan AU - Li N AD - Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming, 650093, China. FAU - Wang, Xuanbing AU - Wang X AD - Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming, 650093, China. FAU - Zhang, Saiyi AU - Zhang S AD - Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming, 650093, China. FAU - Yu, Danni AU - Yu D AD - Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming, 650093, China. FAU - Yang, Yuantao AU - Yang Y AD - Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming, 650093, China. FAU - Zhu, Zhengchao AU - Zhu Z AD - Key Laboratory of Ecological Metallurgy for Polymetallic Symbiotic Ores, Ministry of Education, Northeastern University, Shenyang, 110819, PR China. FAU - Yang, Linjing AU - Yang L AD - Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming, 650093, China. Electronic address: eslinjingyang@kust.edu.cn. FAU - Kim, Jeongwon AU - Kim J AD - Department of Chemistry, Chungnam National University, Daejeon, 34134, Republic of Korea. Electronic address: jwkim@cnu.ac.kr. FAU - Li, Guixiang AU - Li G AD - School of Materials Science and Engineering, Southeast University, Nanjing, Jiangsu, 211189, PR China. Electronic address: guixiang.li@seu.edu.cn. FAU - Xu, Ruidong AU - Xu R AD - Faculty of Metallurgical and Energy Engineering, Kunming University of Science and Technology, Kunming, 650093, China. Electronic address: rdxupaper@aliyun.com. LA - eng PT - Journal Article DEP - 20261023 PL - United States TA - J Colloid Interface Sci JT - Journal of colloid and interface science JID - 0043125 SB - IM OTO - NOTNLM OT - Corrosion strategy OT - Energy-efficient hydrogen production OT - Ethanol electrooxidation OT - Heterostructure electrocatalysis OT - Interfacial reconstruction COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:03 PHST- 2026/07/22 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:03 [entrez] AID - S0021-9797(26)02026-6 [pii] AID - 10.1016/j.jcis.2026.141849 [doi] PST - aheadofprint SO - J Colloid Interface Sci. 2026 Oct 23;729:141849. doi: 10.1016/j.jcis.2026.141849. PMID- 42945672 OWN - NLM STAT- Publisher LR - 20261027 IS - 1096-0023 (Electronic) IS - 1043-4666 (Linking) VI - 208 DP - 2026 Oct 27 TI - Cerebrospinal fluid cytokine profiles in children with acute neurological symptoms. PG - 157224 LID - S1043-4666(26)00119-5 [pii] LID - 10.1016/j.cyto.2026.157224 [doi] AB - INTRODUCTION: In children, acute neurologic symptoms may be a sign either of a severe central nervous system (CNS) disorder or a benign condition, which may be difficult to distinguish from each other by clinical picture. Analysis of cerebrospinal fluid (CSF) cytokine levels may serve as a diagnostic tool and help in distinguishing CNS infections from other conditions. METHOD: Children (n = 144) treated for acute neurologic symptoms between 2014 and 2016 in Turku University Hospital, Finland, were included in the study. Levels of 48 different cytokines were analyzed retrospectively in CSF samples collected during routine patient care. Cytokine levels were compared among children with CNS infection (n = 32; including encephalitis, bacterial meningitis or brain abscess, viral meningitis and Lyme neuroborreliosis), epileptic seizure (n = 32), and migraine (n = 7), as well as between those with or without CNS infection. RESULTS: IL-1ra, MIP-1alpha, RANTES, IL-6, and IL-16 emerged as the most promising candidate diagnostic markers for discriminating children with CNS infection from those without. Their discriminatory performance was moderate, with AUC values ranging from 0.78 to 0.81 (IL-1ra 0.81, MIP-1alpha 0.81, RANTES 0.78, IL-6 0.78, IL-16 0.80). CONCLUSIONS: Determining selected cytokine levels in CSF samples may provide a useful diagnostic tool to aid in distinguishing CNS infections from other conditions in children presenting with similar symptoms. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Poyhonen, Heidi Maria AU - Poyhonen HM AD - Paediatric Neurology, Department of Paediatrics and Adolescent Medicine, Turku University Hospital, Turku, Finland; Department of Paediatrics and Adolescent Medicine, University of Turku, Turku, Finland. Electronic address: hmlaut@utu.fi. FAU - Pietikainen, Kaisa Annukka AU - Pietikainen KA AD - TYKS Laboratories, Clinical Microbiology, Turku University Hospital, Turku, Finland. Electronic address: pietikainen@varha.fi. FAU - Ollila, Helena Emmi Karoliina AU - Ollila HEK AD - Department of Biostatistics, University of Turku, Turku, Finland. Electronic address: helena.e.ollila@utu.fi. FAU - Peltola, Ville Tapani AU - Peltola VT AD - Department of Paediatrics and Adolescent Medicine, University of Turku, Turku, Finland; Department of Paediatrics and Adolescent Medicine, Turku University Hospital, Turku, Finland. Electronic address: vilpel@utu.fi. FAU - Hytonen, Jukka Tapio Johannes AU - Hytonen JTJ AD - TYKS Laboratories, Clinical Microbiology, Turku University Hospital, Turku, Finland; Institute of Biomedicine, University of Turku, Turku, Finland. Electronic address: jukhyt@utu.fi. FAU - Salmi, Marko AU - Salmi M AD - Institute of Biomedicine, University of Turku, Turku, Finland; MediCity Research Laboratory, University of Turku, Turku, Finland; InFLAMES Research Flagship Center, University of Turku, Turku, Finland. Electronic address: ma.salmi@utu.fi. FAU - Lahdesmaki, Tuire Terhikki AU - Lahdesmaki TT AD - Paediatric Neurology, Department of Paediatrics and Adolescent Medicine, Turku University Hospital, Turku, Finland; Department of Paediatrics and Adolescent Medicine, University of Turku, Turku, Finland. Electronic address: tuiolla@utu.fi. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Cytokine JT - Cytokine JID - 9005353 SB - IM OTO - NOTNLM OT - Biomarkers OT - Central nervous system infection OT - Cerebrospinal fluid OT - Cytokines OT - Differential diagnosis OT - Pediatric COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:03 PHST- 2026/03/22 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:03 [entrez] AID - S1043-4666(26)00119-5 [pii] AID - 10.1016/j.cyto.2026.157224 [doi] PST - aheadofprint SO - Cytokine. 2026 Oct 27;208:157224. doi: 10.1016/j.cyto.2026.157224. PMID- 42945656 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-3336 (Electronic) IS - 0304-3894 (Linking) VI - 518 DP - 2026 Oct 23 TI - Fluorinated donor-acceptor polymers blended with solid-state ionic liquids for highly sensitive and selective detection of volatile organic compounds. PG - 144012 LID - S0304-3894(26)02993-6 [pii] LID - 10.1016/j.jhazmat.2026.144012 [doi] AB - Solid-state ionic liquids (SILs), comprising cations with long-alkyl chains and anions containing fluorine atoms, have become essential for achieving electrochemical doping of pi-conjugated polymers (pi-CPs), as the formation of polarons is stabilized by the presence of fluorinated anions under applied bias. Nevertheless, phase separation on the scale of a few hundred nanometers between the polymers and SILs limits efficient doping and hinders intermolecular charge transport. In this study, we present a molecular design approach that incorporates electron-withdrawing fluorine atoms into the isoindigo unit of benzodithiophene-isoindigo (BI) copolymers. This targeted fluorination optimizes the surface energy of the polymer, facilitating the development of well-organized fibrillar nanostructures with improved order and connectivity when mixed with SILs. The optimized morphology enhances the migration of fluorinated anions from the SIL, which in turn promotes efficient electrochemical doping within the fluorinated BI matrix. Additionally, the electrochemical doping process is modulated by alcohol-based gases, yielding chemiresistors with outstanding gas selectivity. Of particular note, the fluorinated BI:SIL chemiresistor demonstrated an impressive response to 1-butanol, reaching a resistance change (DeltaR/R(0)) of 85.28% at 500 ppm and a limit of detection (LOD) of 513.6 ppb. These results underscore the potential of fluorinated pi-CP/SIL blends to serve as adaptable platforms for scalable, selective, and environmentally sustainable organic sensing applications. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Wang, Guanjie AU - Wang G AD - Department of Energy and Materials Engineering, Dongguk University-Seoul, Seoul 04620, Republic of Korea. FAU - Shim, Eun Soo AU - Shim ES AD - Department of Energy and Materials Engineering, Dongguk University-Seoul, Seoul 04620, Republic of Korea. FAU - Lee, Ji Hyeon AU - Lee JH AD - Department of Energy and Materials Engineering, Dongguk University-Seoul, Seoul 04620, Republic of Korea. FAU - Lee, Changhoon AU - Lee C AD - Division of Advanced Materials Science, Pohang University of Science and Technology, Pohang 37673, Republic of Korea; Max Planck POSTECH Center for Complex Phase of Materials, Pohang University of Science and Technology, Pohang 37673, Republic of Korea. FAU - Ahn, Hyungju AU - Ahn H AD - Pohang Accelerator Laboratory, Pohang University of Science and Technology, Pohang 37673, Republic of Korea. FAU - Balamurugan, Chandran AU - Balamurugan C AD - Department of Energy and Materials Engineering, Dongguk University-Seoul, Seoul 04620, Republic of Korea. FAU - Wang, Zhongwu AU - Wang Z AD - State Key Laboratory of Advanced Materials for Intelligent Sensing & Key Laboratory of Organic Integrated Circuit, Ministry of Education & Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, Institute of Molecular Aggregation Science, Tianjin University, Tianjin 300072, China. FAU - Li, Liqiang AU - Li L AD - State Key Laboratory of Advanced Materials for Intelligent Sensing & Key Laboratory of Organic Integrated Circuit, Ministry of Education & Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, Institute of Molecular Aggregation Science, Tianjin University, Tianjin 300072, China. FAU - Lee, Hyeonryul AU - Lee H AD - Department of Advanced battery Convergence Engineering, Dongguk University-Seoul, Seoul 04620, Republic of Korea. FAU - Sung, Junyeong AU - Sung J AD - Department of Energy and Materials Engineering, Dongguk University-Seoul, Seoul 04620, Republic of Korea. FAU - Lee, Sungmin AU - Lee S AD - Department of Energy and Materials Engineering, Dongguk University-Seoul, Seoul 04620, Republic of Korea. FAU - Shim, Ji Hoon AU - Shim JH AD - Division of Advanced Materials Science, Pohang University of Science and Technology, Pohang 37673, Republic of Korea; Department of Chemistry, Pohang University of Science and Technology, Pohang 37673, Republic of Korea. FAU - Jo, Jea Woong AU - Jo JW AD - Department of Energy and Materials Engineering, Dongguk University-Seoul, Seoul 04620, Republic of Korea. Electronic address: whwp78@dongguk.edu. FAU - Kwon, Sooncheol AU - Kwon S AD - Department of Energy and Materials Engineering, Dongguk University-Seoul, Seoul 04620, Republic of Korea. Electronic address: kwansc12@dongguk.edu. LA - eng PT - Journal Article DEP - 20261023 PL - Netherlands TA - J Hazard Mater JT - Journal of hazardous materials JID - 9422688 SB - IM OTO - NOTNLM OT - Benzodithiophene-isoindigo OT - Electrochemical doping OT - Solid-state ionic liquids OT - Surface energy control OT - VOCs chemiresistors COIS- Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Sooncheol Kwon reports financial support was provided by Korea Institute of Energy Technology Evaluation and Planning (KETEP), and the Ministry of Trade, Industry & Energy (MOTIE) of the Republic of Korea. Jea Woong Jo reports financial support was provided by National Research Foundation of Korea. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:03 PHST- 2025/12/20 00:00 [received] PHST- 2026/08/19 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:03 [entrez] AID - S0304-3894(26)02993-6 [pii] AID - 10.1016/j.jhazmat.2026.144012 [doi] PST - aheadofprint SO - J Hazard Mater. 2026 Oct 23;518:144012. doi: 10.1016/j.jhazmat.2026.144012. PMID- 42945647 OWN - NLM STAT- Publisher LR - 20261027 IS - 0151-9638 (Print) IS - 0151-9638 (Linking) VI - 153 IP - 4 DP - 2026 Sep 22 TI - Ochre fluorescence as a marker of invasive melanoma under ultraviolet-induced fluorescence dermoscopy. PG - 103532 LID - S0151-9638(26)00066-9 [pii] LID - 10.1016/j.annder.2026.103532 [doi] FAU - Litaiem, N AU - Litaiem N AD - Department of Dermatology, Charles Nicolle Hospital, Tunis, Tunisia; University of Tunis El Manar, Faculte de Medecine de Tunis, Tunisia. Electronic address: noureddine.litaiem@gmail.com. FAU - Frikha, M AU - Frikha M AD - Department of Dermatology, Charles Nicolle Hospital, Tunis, Tunisia; University of Tunis El Manar, Faculte de Medecine de Tunis, Tunisia. FAU - Gara, S AU - Gara S AD - Department of Dermatology, Charles Nicolle Hospital, Tunis, Tunisia; University of Tunis El Manar, Faculte de Medecine de Tunis, Tunisia. FAU - Slouma, M AU - Slouma M AD - University of Tunis El Manar, Faculte de Medecine de Tunis, Tunisia; Pain Management Center, La Rabta Hospital, Tunis, Tunisia. FAU - Jones, M AU - Jones M AD - Department of Dermatology, Charles Nicolle Hospital, Tunis, Tunisia; University of Tunis El Manar, Faculte de Medecine de Tunis, Tunisia. FAU - Zeglaoui, F AU - Zeglaoui F AD - Department of Dermatology, Charles Nicolle Hospital, Tunis, Tunisia; University of Tunis El Manar, Faculte de Medecine de Tunis, Tunisia. LA - eng PT - Journal Article DEP - 20260922 PL - France TA - Ann Dermatol Venereol JT - Annales de dermatologie et de venereologie JID - 7702013 SB - IM COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:03 PHST- 2026/03/07 00:00 [received] PHST- 2026/05/01 00:00 [revised] PHST- 2026/08/26 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:03 [entrez] AID - S0151-9638(26)00066-9 [pii] AID - 10.1016/j.annder.2026.103532 [doi] PST - aheadofprint SO - Ann Dermatol Venereol. 2026 Sep 22;153(4):103532. doi: 10.1016/j.annder.2026.103532. PMID- 42945643 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-426X (Electronic) IS - 0008-6215 (Linking) VI - 570 DP - 2026 Oct 12 TI - Synthesis of 2,3-dideoxy-C-3-thiopyrimidine based new Glycohybrids. PG - 110124 LID - S0008-6215(26)00313-7 [pii] LID - 10.1016/j.carres.2026.110124 [doi] AB - The stereoselective synthesis of C-3-thiopyrimidine-based glycohybrids remains largely under explored. An efficient and highly stereoselective one-pot organocatalytic strategy has been developed for the synthesis of new 2,3-dideoxy-C-3-thiopyrimidine-based C-3-thio-glycohybrids from Perlin's aldehydes and pyrimidine-2-thione derivatives under mild, metal-free conditions. l-proline used as an inexpensive organocatalyst, the reaction proceeds smoothly at room temperature to afford twenty new glycohybrids in good to very good yields with excellent equatorial selectivity at C-3. The structures and stereochemical configurations of the products were established by spectroscopic analyses and single-crystal X-ray diffraction studies. The operational simplicity, metal-free nature, and high stereoselectivity make this protocol an attractive and sustainable approach for accessing privileged C-3-thioglycohybrids. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Sharma, Sunil AU - Sharma S AD - Glycochemistry Laboratory, School of Physical Sciences, Jawaharlal Nehru University, New Delhi, 110067, India. FAU - Shukla, Anurag AU - Shukla A AD - Glycochemistry Laboratory, School of Physical Sciences, Jawaharlal Nehru University, New Delhi, 110067, India. FAU - Sagar, Ram AU - Sagar R AD - Glycochemistry Laboratory, School of Physical Sciences, Jawaharlal Nehru University, New Delhi, 110067, India. Electronic address: ram.sagar@jnu.ac.in. LA - eng PT - Journal Article DEP - 20261012 PL - Netherlands TA - Carbohydr Res JT - Carbohydrate research JID - 0043535 SB - IM COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:03 PHST- 2026/07/14 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:03 [entrez] AID - S0008-6215(26)00313-7 [pii] AID - 10.1016/j.carres.2026.110124 [doi] PST - aheadofprint SO - Carbohydr Res. 2026 Oct 12;570:110124. doi: 10.1016/j.carres.2026.110124. PMID- 42945631 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-3557 (Electronic) IS - 1386-1425 (Linking) VI - 367 IP - Pt 2 DP - 2026 Oct 19 TI - Ligand-engineered copper nanoclusters combined with carbon dots as a ratiometric probe for brilliant blue detection and information security. PG - 129007 LID - S1386-1425(26)01578-7 [pii] LID - 10.1016/j.saa.2026.129007 [doi] AB - Brilliant blue (BB) is a common food dye that can poses health risks at high intake, necessitating hypersensitive analysis. To this end, 6-Fluoro-2-mercaptobenzothiazole (6-F-MBT) was identified as the optimal ligand through comparative studies with its isomers and substituent-modified analogues. Then orange-emitting copper nanoclusters (6-F-MBT@PVP-CuNCs) were fabricated via an optimized hydrothermal route with 6-F-MBT and polyvinylpyrrolidone (PVP) as dual ligands, and later assembled with blue-emitting carbon dots (CDs) to yield a ratiometric fluorescent (RF) probe that could selectively monitor BB. The ratiometric detection relied on combined FRET and dynamic quenching, which together provided a limit of detection (LOD) of 33.5 nM and a linear range spanning 0.1 to 34.4 muM. It also supports solution-phase quantification via smartphone RGB analysis (1.4-44.8 muM) and on-site assay using paper-based strips (2.0-50.0 muM). Besides, the 6-F-MBT@PVP-CuNCs possess bright fluorescence and superior stability, holding promise for anti-tampering and finger mark recognition. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Zhou, Yi AU - Zhou Y AD - Precise Synthesis and Function Development Key Laboratory of Sichuan Province, China West Normal University, Nanchong, 637002, China. FAU - Peng, Xinyi AU - Peng X AD - Precise Synthesis and Function Development Key Laboratory of Sichuan Province, China West Normal University, Nanchong, 637002, China. FAU - Huang, Feng AU - Huang F AD - Precise Synthesis and Function Development Key Laboratory of Sichuan Province, China West Normal University, Nanchong, 637002, China. FAU - Li, Li AU - Li L AD - Precise Synthesis and Function Development Key Laboratory of Sichuan Province, China West Normal University, Nanchong, 637002, China. FAU - Zeng, Miao AU - Zeng M AD - Aba Teachers College, Wenchuan, Sichuan, 623002, China. Electronic address: 664928619@qq.com. FAU - Cheng, Zhengjun AU - Cheng Z AD - Precise Synthesis and Function Development Key Laboratory of Sichuan Province, China West Normal University, Nanchong, 637002, China; College of Chemistry and Chemical Engineering, China West Normal University, Nanchong, 637002, China. Electronic address: ncczj1112@126.com. LA - eng PT - Journal Article DEP - 20261019 PL - England TA - Spectrochim Acta A Mol Biomol Spectrosc JT - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JID - 9602533 SB - IM OTO - NOTNLM OT - Anti-counterfeiting OT - Copper nanoclusters OT - Latent fingerprints OT - Quenching mechanism OT - Visual detection COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:02 PHST- 2026/08/30 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:02 [entrez] AID - S1386-1425(26)01578-7 [pii] AID - 10.1016/j.saa.2026.129007 [doi] PST - aheadofprint SO - Spectrochim Acta A Mol Biomol Spectrosc. 2026 Oct 19;367(Pt 2):129007. doi: 10.1016/j.saa.2026.129007. PMID- 42945630 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-3557 (Electronic) IS - 1386-1425 (Linking) VI - 367 IP - Pt 2 DP - 2026 Oct 17 TI - Three-dimensional nanogap substrate for metal-enhanced fluorescence-based ultra-sensitive detection of trace ammonia. PG - 128993 LID - S1386-1425(26)01564-7 [pii] LID - 10.1016/j.saa.2026.128993 [doi] AB - High sensitivity and rapid detection of trace ammonia are crucial for environmental monitoring and disease diagnosis. Polydiacetylene (PDA)-based sensors have attracted significant attention due to their fast response; however, weak fluorescence signals under low ammonia concentrations limit their performance. To address this issue, this study further developed a PDA-based ammonia sensing platform based on a three-dimensional (3D) silver nanogap substrate by utilizing the metal-enhanced fluorescence (MEF) effect to enhance the fluorescence response of PDA. Specifically, a nanopillar array with a diameter of 250 nm, a height of 100 nm, and a period of 500 nm was fabricated on a glass slide using ultraviolet nanoimprint lithography. After that, an oxygen plasma treatment was applied to form a nanogap network, and a 75 nm thick silver layer was deposited on the surface via physical vapor deposition (PVD) to create a 3D silver nanogap structure, thus constructing the 3D nanogap substrate. Following this, PDA vesicles were then immobilized on the substrate surface for ammonia sensing. Experimental results demonstrated that, under static sealed-chamber conditions, the 3D nanogap substrate achieved a limit of detection (LOD) of 4.79 ppm for ammonia, calculated using the standard 3sigma/k method based on the theoretically estimated ammonia concentration. In addition, the sensor exhibited excellent selectivity toward ammonia compared with volatile organic compounds such as toluene, ethanol, and methanol, demonstrating great potential for trace ammonia detection applications. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Qi, Longchao AU - Qi L AD - Department of Mechanical Engineering, Yanbian University, Yanji, Jilin, 133002, China. FAU - Hu, Hongzhong AU - Hu H AD - Department of Mechanical Engineering, Yanbian University, Yanji, Jilin, 133002, China. FAU - Zhang, Gude AU - Zhang G AD - Department of Chemistry, National Demonstration Centre for Experimental Chemistry Education, Yanbian University, Yanji, Jilin, 133002, China. FAU - Cui, Chunzhi AU - Cui C AD - Department of Chemistry, National Demonstration Centre for Experimental Chemistry Education, Yanbian University, Yanji, Jilin, 133002, China. Electronic address: cuichunzhi@ybu.edu.cn. FAU - Lu, Xun AU - Lu X AD - Department of Mechanical Engineering, Yanbian University, Yanji, Jilin, 133002, China. Electronic address: luxun@ybu.edu.cn. LA - eng PT - Journal Article DEP - 20261017 PL - England TA - Spectrochim Acta A Mol Biomol Spectrosc JT - Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy JID - 9602533 SB - IM OTO - NOTNLM OT - Metal enhanced fluorescence OT - Polydiacetylene OT - Sensor OT - Silver nanostructure OT - ammonia detection COIS- Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Xun Lu reports financial support was provided by National Natural Science Foundation of China (Grant No. 32560233). If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:02 PHST- 2026/07/21 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:02 [entrez] AID - S1386-1425(26)01564-7 [pii] AID - 10.1016/j.saa.2026.128993 [doi] PST - aheadofprint SO - Spectrochim Acta A Mol Biomol Spectrosc. 2026 Oct 17;367(Pt 2):128993. doi: 10.1016/j.saa.2026.128993. PMID- 42945626 OWN - NLM STAT- Publisher LR - 20261027 IS - 1872-7603 (Electronic) IS - 0165-0378 (Linking) VI - 178 DP - 2026 Oct 2 TI - Reduced SPARC in trophoblast promotes decidual M1 macrophage polarization and trophoblast dysfunction. PG - 104975 LID - S0165-0378(26)00144-0 [pii] LID - 10.1016/j.jri.2026.104975 [doi] AB - Missed abortion (MA) is a complex pregnancy complication with multifactorial etiologies, including embryonic chromosomal anomalies and maternal endocrine disorders. Among these, immune dysregulation at the maternal-fetal interface has been recognized as a critical contributing factor. Secreted protein acidic and rich in cysteine (SPARC) is implicated in macrophage polarization and trophoblast function, but its role in MA-associated immune imbalance remains unclear. Decidual tissues were collected from 7 to 10 week MA patients and gestational age-matched healthy controls who underwent induced abortions. SPARC expression and macrophage phenotypes were assessed by Western blot and multiplex immunofluorescence staining for SPARC, CD68, CD86, and CD206. In vitro, THP-1 monocytes were differentiated into macrophages by PMA, which were then exposed to the conditioned media collected from SPARC-knockdown or control HTR8/SVneo trophoblasts. Cell viability, migration and apoptosis of HTR8/SVneo cells were evaluated under indirect co-culture with macrophages, with or without TNF-alpha neutralizing antibody. SPARC expression was significantly reduced in MA decidua, and its expression negatively correlated with the proportion of M1 macrophages, while positively correlated with M2 macrophages. SPARC knockdown in trophoblasts promoted macrophages M1 polarization, elevated TNF-alpha, and impaired macrophage-induced trophoblast viability, invasion, and survival. TNF-alpha neutralization partially rescued these effects. Mechanistically, SPARC promoted M2 polarization via the AKT/SP1 pathway, and its knockdown suppressed Akt phosphorylation and SP1 expression. Reduced SPARC in MA decidua drives M1 macrophage dominance and TNF-alpha-mediated trophoblast dysfunction through AKT/SP1 signaling, highlighting SPARC as a potential therapeutic target for MA. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Liu, Junmei AU - Liu J AD - Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, 107 Wenhua Xi Road, Jinan, Shandong, 250012, PR China. FAU - Ding, Jiayue AU - Ding J AD - Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, 107 Wenhua Xi Road, Jinan, Shandong, 250012, PR China. FAU - Su, Zixuan AU - Su Z AD - Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, 107 Wenhua Xi Road, Jinan, Shandong, 250012, PR China. FAU - Xu, Yintao AU - Xu Y AD - Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, 107 Wenhua Xi Road, Jinan, Shandong, 250012, PR China. FAU - Li, Yu AU - Li Y AD - State Key Laboratory of Reproductive Medicine and Offspring Health, Center for Reproductive Medicine, Institute of Women, Children and Reproductive Health, Shandong University, 250012, China. FAU - Fang, Yan AU - Fang Y AD - Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, 107 Wenhua Xi Road, Jinan, Shandong, 250012, PR China. Electronic address: fangyan@sdu.edu.cn. LA - eng PT - Journal Article DEP - 20261002 PL - Ireland TA - J Reprod Immunol JT - Journal of reproductive immunology JID - 8001906 SB - IM OTO - NOTNLM OT - Macrophages OT - Missed abortion OT - SPARC OT - TNF-alpha OT - Trophoblasts COIS- Declaration of Competing interest The author(s) declare(s) that they have no competing interests EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:02 PHST- 2026/05/08 00:00 [received] PHST- 2026/08/27 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:02 [entrez] AID - S0165-0378(26)00144-0 [pii] AID - 10.1016/j.jri.2026.104975 [doi] PST - aheadofprint SO - J Reprod Immunol. 2026 Oct 2;178:104975. doi: 10.1016/j.jri.2026.104975. PMID- 42945619 OWN - NLM STAT- Publisher LR - 20261027 IS - 1095-6859 (Electronic) IS - 0090-8258 (Linking) VI - 215 DP - 2026 Oct 27 TI - LMOD1 expression and survival in uterine leiomyosarcoma: discordant results in tissue microarrays and whole sections. PG - 7-15 LID - S0090-8258(26)02172-4 [pii] LID - 10.1016/j.ygyno.2026.10.018 [doi] AB - BACKGROUND: Leiomodin 1 (LMOD1) was previously reported to be associated with longer survival in leiomyosarcoma (LMS) in analysis combining uterine and non-uterine tumors. The objective of the present study was to analyze the expression and prognostic role of LMOD1 in a large series of exclusively uterine LMS (uLMS). METHODS: LMOD1 protein expression by immunohistochemistry was analyzed in tissue microarrays (TMAs) from 244 uLMS and whole sections from 203 uLMS, of which 134 were included in both series. Expression was assessed for association with clinicopathologic parameters and disease-specific survival (DSS). RESULTS: TMAs were informative in 221/244 (91%) tumors, of which 100 (45%) expressed LMOD1. No significant association with DSS was observed for 156 patients with clinical data. In the series stained using whole sections, LMOD1 was expressed in 142/203 (70%) tumors, and higher expression was associated with significantly longer survival (203 patients with survival data; p=0.007). Other parameters related to survival in the latter series were FIGO stage (p<0.001), tumor diameter (p<0.001), mitotic count (p<0.001), the degree of atypia (p=0.006) and lymphovascular invasion (LVSI; p=0.006). In Cox multivariate analysis, FIGO stage (p<0.001), mitotic count (p<0.001), tumor diameter (p=0.001) and LMOD1 expression (p=0.003) were independent prognosticators. CONCLUSIONS: LMOD1 expression in whole sections is a new candidate marker of longer survival in uLMS. This study additionally highlights the limitations associated with use of TMA sections compared to whole sections in biomarker studies. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Davidson, Ben AU - Davidson B AD - Department of Pathology, Oslo University Hospital, Norwegian Radium Hospital, Oslo, N-0310, Norway; University of Oslo, Faculty of Medicine, Institute of Clinical Medicine, Oslo, N-0316, Norway. Electronic address: bend@medisin.uio.no. FAU - Holth, Arild AU - Holth A AD - Department of Pathology, Oslo University Hospital, Norwegian Radium Hospital, Oslo, N-0310, Norway. FAU - Horni Gloersen, Guro AU - Horni Gloersen G AD - Department of Pathology, Oslo University Hospital, Norwegian Radium Hospital, Oslo, N-0310, Norway. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Gynecol Oncol JT - Gynecologic oncology JID - 0365304 SB - IM OTO - NOTNLM OT - Immunohistochemistry OT - LMOD1 OT - Prognosis OT - Uterine leiomyosarcoma COIS- Declaration of competing interest The authors have no conflicts of interest to declare. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 19:02 PHST- 2026/06/18 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 19:02 [entrez] AID - S0090-8258(26)02172-4 [pii] AID - 10.1016/j.ygyno.2026.10.018 [doi] PST - aheadofprint SO - Gynecol Oncol. 2026 Oct 27;215:7-15. doi: 10.1016/j.ygyno.2026.10.018. PMID- 42945609 OWN - NLM STAT- Publisher LR - 20261027 IS - 1879-0291 (Electronic) IS - 0141-1136 (Linking) VI - 223 DP - 2026 Oct 26 TI - Physiological responses of the invasive mussel Mytella strigata to atmospheric heatwaves under a simulated tidal cycle. PG - 108509 LID - S0141-1136(26)00678-1 [pii] LID - 10.1016/j.marenvres.2026.108509 [doi] AB - Atmospheric heatwaves increasingly threaten intertidal bivalves and ecosystems they support, yet the thermal responses of the highly invasive mussel Mytella strigata remain poorly understood. In this study, mussels were exposed to a simulated tidal regime for five days, with aerial temperatures of 32  degrees C (control) or 40  degrees C (heatwave) during low tide. Transcriptomic analysis identified 2497 DEGs, including 1157 upregulated and 1340 downregulated genes. These genes were primarily enriched in protein processing in the endoplasmic reticulum, MAPK signaling, oxidative phosphorylation, and carbohydrate metabolism. Heat shock proteins and endoplasmic-reticulum quality-control genes were upregulated, indicating enhanced proteostasis maintenance. The upregulation of genes involved in the mitochondrial respiratory chain suggested an increased capacity for ATP production. Meanwhile, the differential expression of cellulase, beta-glucosidase, and phosphoglucomutase indicated substantial carbohydrate metabolic remodeling. Our findings suggest that M. strigata responds to heatwave exposure through coordinated proteostasis regulation, metabolic remodeling, and antioxidant defense. Increased catalase activity accompanied by reduced malondialdehyde content suggests that antioxidant compensation effectively limited lipid peroxidation, potentially contributing to the thermal tolerance and persistence of this invasive mussel. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Zeng, Wanqing AU - Zeng W AD - Fisheries College, Guangdong Ocean University, Zhanjiang, China. FAU - Liang, Xinming AU - Liang X AD - Fisheries College, Guangdong Ocean University, Zhanjiang, China. FAU - Xu, Xin AU - Xu X AD - Fisheries College, Guangdong Ocean University, Zhanjiang, China. FAU - Liu, Yong AU - Liu Y AD - Pearl Oyster Research Institute, Guangdong Ocean University, Zhanjiang, China. FAU - Zhao, Liqiang AU - Zhao L AD - Fisheries College, Guangdong Ocean University, Zhanjiang, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Guangdong Ocean University, Zhanjiang, China; Key Laboratory of Marine Ecology and Aquaculture Environment of Zhanjiang, Zhanjiang, China. Electronic address: lzhao@gdou.edu.cn. LA - eng PT - Journal Article DEP - 20261026 PL - England TA - Mar Environ Res JT - Marine environmental research JID - 9882895 SB - IM OTO - NOTNLM OT - Atmospheric heatwaves OT - Bivalves OT - Energy metabolism OT - Proteostasis OT - Transcriptomics COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 18:59 PHST- 2026/07/22 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/24 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 18:59 [entrez] AID - S0141-1136(26)00678-1 [pii] AID - 10.1016/j.marenvres.2026.108509 [doi] PST - aheadofprint SO - Mar Environ Res. 2026 Oct 26;223:108509. doi: 10.1016/j.marenvres.2026.108509. PMID- 42945606 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-3360 (Electronic) IS - 0306-4530 (Linking) VI - 195 DP - 2026 Sep 29 TI - Early-life clonazepam exposure induces long-lasting molecular and behavioral alterations in hippocampal circuits in male rats. PG - 108034 LID - S0306-4530(26)00294-5 [pii] LID - 10.1016/j.psyneuen.2026.108034 [doi] AB - Benzodiazepines are widely prescribed during early life; however, their long-term impact on hippocampal neurochemical maturation and memory function remains poorly understood. We investigated whether neonatal exposure to clonazepam (CLZ) induces persistent molecular and behavioral alterations in hippocampal circuits and episodic-like memory (ELM). Male rats were exposed to CLZ or vehicle from postnatal day (PND) 7-11. CLZ exposure selectively impaired the temporal ("when") component of ELM, whereas spatial discrimination remained preserved. CLZ-treated animals exhibited reduced expression of steroidogenic acute regulatory protein, 5alpha-reductase type 1 and 3alpha-hydroxysteroid dehydrogenase in the dentate gyrus and/or CA1, suggesting potential alterations in local neurosteroid synthesis. Some of these changes were accompanied by modifications in DNA methylation. Additionally, region-specific alterations in N-methyl-D-aspartate (NMDA) receptor subunits were observed, including increased GluN1 mRNA expression in CA3 and reduced GluN2A, GluN2B, and GluN3A gene expression in CA1. Together, these findings indicate that early-life CLZ exposure produces long-lasting, circuit-specific molecular adaptations in the hippocampus and selective impairment of the temporal component of ELM. Our results highlight the vulnerability of developing hippocampal networks to pharmacological modulation during critical developmental windows. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Rossetti, Maria Florencia AU - Rossetti MF AD - Instituto de Salud y Ambiente del Litoral (ISAL), Facultad de Bioquimica y Ciencias Biologicas, Universidad Nacional del Litoral-CONICET, Santa Fe, Argentina. Electronic address: mfrossetti@fbcb.unl.edu.ar. FAU - Fernandez, Pamela AU - Fernandez P AD - Instituto de Salud y Ambiente del Litoral (ISAL), Facultad de Bioquimica y Ciencias Biologicas, Universidad Nacional del Litoral-CONICET, Santa Fe, Argentina; Catedra de Nutricion en Situaciones Patologicas, Facultad de Bioquimica y Ciencias Biologicas, Universidad Nacional del Litoral, Santa Fe, Argentina. Electronic address: pame.fernandez@live.com. FAU - Canesini, Guillermina AU - Canesini G AD - Instituto de Salud y Ambiente del Litoral (ISAL), Facultad de Bioquimica y Ciencias Biologicas, Universidad Nacional del Litoral-CONICET, Santa Fe, Argentina; Catedra de Nutricion en Situaciones Patologicas, Facultad de Bioquimica y Ciencias Biologicas, Universidad Nacional del Litoral, Santa Fe, Argentina. Electronic address: gcanesini@fbcb.unl.edu.ar. FAU - Diaz, Daniel O AU - Diaz DO AD - Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, Rosario, Santa Fe, Argentina. Electronic address: farmaciasdiazz@gmail.com. FAU - Stoker, Cora AU - Stoker C AD - Instituto de Salud y Ambiente del Litoral (ISAL), Facultad de Bioquimica y Ciencias Biologicas, Universidad Nacional del Litoral-CONICET, Santa Fe, Argentina; Departamento de Bioquimica Clinica y Cuantitativa, Facultad de Bioquimica y Ciencias Biologicas, Universidad Nacional del Litoral, Santa Fe, Argentina. Electronic address: cstoker@fbcb.unl.edu.ar. FAU - Ramos, Jorge Guillermo AU - Ramos JG AD - Instituto de Salud y Ambiente del Litoral (ISAL), Facultad de Bioquimica y Ciencias Biologicas, Universidad Nacional del Litoral-CONICET, Santa Fe, Argentina; Departamento de Bioquimica Clinica y Cuantitativa, Facultad de Bioquimica y Ciencias Biologicas, Universidad Nacional del Litoral, Santa Fe, Argentina. Electronic address: gramos@fbcb.unl.edu.ar. LA - eng PT - Journal Article DEP - 20260929 PL - England TA - Psychoneuroendocrinology JT - Psychoneuroendocrinology JID - 7612148 SB - IM OTO - NOTNLM OT - Clonazepam OT - DNA methylation OT - Episodic-like memory OT - Hippocampus OT - Neurosteroidogenic enzymes COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 18:59 PHST- 2026/06/05 00:00 [received] PHST- 2026/08/19 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 18:59 [entrez] AID - S0306-4530(26)00294-5 [pii] AID - 10.1016/j.psyneuen.2026.108034 [doi] PST - aheadofprint SO - Psychoneuroendocrinology. 2026 Sep 29;195:108034. doi: 10.1016/j.psyneuen.2026.108034. PMID- 42945605 OWN - NLM STAT- Publisher LR - 20261027 IS - 1872-8421 (Electronic) IS - 0165-5728 (Linking) VI - 422 DP - 2026 Oct 24 TI - Altered HMGB1-TLR4-NF-kappaB Signaling and regulatory microRNA expression in idiopathic generalized epilepsy according to Toxoplasma gondii serostatus. PG - 579114 LID - S0165-5728(26)00263-8 [pii] LID - 10.1016/j.jneuroim.2026.579114 [doi] AB - AIM: To investigate inflammatory signaling pathways and regulatory microRNA expression profiles in patients with idiopathic generalized epilepsy according to Toxoplasma gondii serostatus. The novelty of this study lies in the integrated evaluation of the HMGB1-TLR4-RAGE-IRAK-TRAF6-NF-kappaB signaling axis together with selected regulatory microRNAs across groups stratified according to both epilepsy diagnosis and T. gondii serostatus. METHODS: A total of 40 participants aged 18-50 years were enrolled and classified into four predefined groups (n = 10 each): control [epilepsy (-)/T. gondii (-)], epilepsy [epilepsy (+)/T. gondii (-)], T. gondii [epilepsy (-)/T. gondii (+)], and epilepsy + T. gondii [epilepsy (+)/T. gondii (+)]. Serum concentrations of HMGB1, NF-kappaB, TLR4, TNF-alpha, TRAF6, RAGE, and IL-1beta were measured using ELISA. Plasma expression levels of HMGB1, IL1B, IL10, IRAK1, IRAK2, TLR4, TNFA, and RAGE mRNAs, as well as miR-146a, miR-155, miR-26b, and miR-106b, were determined by quantitative real-time PCR. Group comparisons were performed using the Kruskal-Wallis test, and associations between variables were evaluated using Spearman correlation analysis. RESULTS: IL1B gene expression was significantly increased in the T. gondii group compared with the epilepsy and epilepsy + T. gondii groups (p < 0.05), whereas HMGB1 gene expression was significantly lower in the T. gondii group than in the other study groups (p < 0.05). IRAK2 expression was significantly elevated in the epilepsy + T. gondii group compared with controls (p < 0.05). Serum HMGB1 levels were higher in the T. gondii group [5.87 (3.42-8.69)] than in the epilepsy group [3.10 (2.70-6.70)], and serum TNF-alpha levels were similarly higher in the T. gondii group [279 (83.47-416.57)] than in the epilepsy group [79 (28-279)] (p < 0.05). Among the investigated microRNAs, plasma miR-155 expression was higher in the T. gondii group [4.50 (2.50-9.51)] than in the control [1.26 (0.23-2.67)] and epilepsy [3.36 (0.63-5.81)] groups, whereas miR-106b expression was lower in the epilepsy group [2.42 (0.07-3.99)] than in the other study groups (p < 0.05). Correlation analyses demonstrated significant associations among components of the HMGB1-TLR4-NF-kappaB inflammatory signaling pathway and selected regulatory microRNAs. CONCLUSION: The findings of this exploratory study demonstrate differences in selected inflammatory signaling components and regulatory microRNA expression profiles across groups defined by idiopathic generalized epilepsy and T. gondii serostatus. In particular, significant alterations were observed in IL1B, HMGB1, IRAK2, miR-155, and miR-106b expression, together with differences in serum HMGB1 and TNF-alpha levels. However, given the small sample size, cross-sectional design, and predefined group allocation, these findings should be considered exploratory and do not establish a causal relationship between T. gondii infection and epilepsy. Larger prospective studies are required to determine the clinical and pathophysiological significance of these molecular alterations. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Soyturk, Hayriye AU - Soyturk H AD - Bolu Abant Izzet Baysal University, Institute of Graduate Studies, Interdisciplinary Neuroscience, Bolu, Turkiye. Electronic address: hayriyeorallar@ibu.edu.tr. FAU - Kilic, Umit AU - Kilic U AD - Afyonkarahisar Health Sciences University, Faculty of Health Sciences, Department of Physiotherapy and Rehabilitation, Afyonkarahisar, Turkiye. Electronic address: umit.kilic@afsu.edu.tr. FAU - Aydin Turkoglu, Sule AU - Aydin Turkoglu S AD - Bolu Abant Izzet Baysal University, Faculty of Medicine, Department of Neurology, Bolu, Turkiye. FAU - Demir, Serif AU - Demir S AD - Duzce University, Faculty of Medicine, Department of Physiology, Duzce, Turkiye. Electronic address: serifdemir@duzce.edu.tr. LA - eng PT - Journal Article DEP - 20261024 PL - Netherlands TA - J Neuroimmunol JT - Journal of neuroimmunology JID - 8109498 SB - IM OTO - NOTNLM OT - HMGB1 OT - Idiopathic generalized epilepsy OT - NF-kappaB OT - Neuroinflammation OT - TLR4 OT - Toxoplasma gondii OT - microRNA COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 18:59 PHST- 2026/06/19 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/10/24 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 18:59 [entrez] AID - S0165-5728(26)00263-8 [pii] AID - 10.1016/j.jneuroim.2026.579114 [doi] PST - aheadofprint SO - J Neuroimmunol. 2026 Oct 24;422:579114. doi: 10.1016/j.jneuroim.2026.579114. PMID- 42945603 OWN - NLM STAT- Publisher LR - 20261027 IS - 1532-1983 (Electronic) IS - 0261-5614 (Linking) VI - 67 DP - 2026 Sep 2 TI - Late-timed eating alters the diurnal pattern of fuel selection and lowers metabolic flexibility without changing 24-hour energy expenditure: A randomized crossover trial. PG - 106771 LID - S0261-5614(26)00198-6 [pii] LID - 10.1016/j.clnu.2026.106771 [doi] AB - BACKGROUND & AIMS: Meal timing may influence energy expenditure (EE) and metabolic responses. However, the effects of different meal-timing schedules on free-living physical activity, 24-h EE, respiratory exchange ratio (RER), and metabolic flexibility under matched energy intake and meal composition remain unclear. METHODS: In a randomized two-period crossover trial, eight healthy young men were allocated in a 1:1 ratio to either an early-to-late or late-to-early meal-timing sequence, with a washout period of at least 1 week between conditions. Participants followed each meal schedule for 7 days and stayed in a whole-room calorimetry chamber from the evening of Day 7 to the morning of Day 9. Identical meals were provided under both conditions, with matched energy intake, meal composition, fasting duration, and sleep-wake timing. Twenty-four-hour EE and RER were assessed from the morning of Day 8 to the morning of Day 9, while free-living physical activity and accelerometer-derived activity-related EE were assessed on Days 1-6. RESULTS: Accelerometer-derived estimates of activity-related EE during the free-living period did not differ between the early- and late-timed eating conditions (651 +/- 112 vs. 656 +/- 178 kcal/d, P = 0.922), and no significant differences were observed in 24-h step counts or time spent in different activity-intensity categories. Total 24-h EE did not differ significantly between early and late meal timing conditions (1879 +/- 194 vs. 1856 +/- 229; p = 0.489). Although the 24-h mean RER was slightly higher in the late-timed condition (0.895 +/- 0.019) than in the early-timed condition (0.879 +/- 0.017), the difference did not reach statistical significance (p = 0.106, effect size d = 0.832). In contrast, RER during the sleep period (00:00-07:59) was significantly higher in the late-timed condition (0.897 +/- 0.017) compared to early-timed eating (0.838 +/- 0.027; p < 0.001). Moreover, the amplitude of 24-h RER variation-used as a surrogate for metabolic flexibility-was significantly lower with late-timed eating (0.131 +/- 0.021 vs. 0.194 +/- 0.030; p < 0.001). CONCLUSION: In this 8-day controlled meal-timing intervention, late-timed eating did not alter free-living physical activity or accelerometer-derived activity-related EE, nor did it alter 24-h EE measured in the room chamber. However, it was associated with a higher sleep-period RER and a smaller 24-h RER range, consistent with lower metabolic flexibility and an attenuated overnight shift in fuel selection from carbohydrate toward fat. CI - Copyright (c) 2026. Published by Elsevier Ltd. FAU - Hatamoto, Yoichi AU - Hatamoto Y AD - The Fukuoka University Institute for Physical Activity, Japan 8-19-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan; Center for Clinical Nutrition, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, 3-17 Senrioka Shinmachi, Settsu City, Osaka, Japan; Microbial Research Center for Health and Medicine, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Osaka, Japan. Electronic address: yhatamoto@nibn.go.jp. FAU - Tanoue, Yukiya AU - Tanoue Y AD - The Fukuoka University Institute for Physical Activity, Japan 8-19-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan; Center for Clinical Nutrition, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, 3-17 Senrioka Shinmachi, Settsu City, Osaka, Japan. FAU - Yoshimura, Eiichi AU - Yoshimura E AD - Center for Clinical Nutrition, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, 3-17 Senrioka Shinmachi, Settsu City, Osaka, Japan; Microbial Research Center for Health and Medicine, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki, Osaka, Japan. Electronic address: eyoshi@nibn.go.jp. FAU - Ikaga, Reina AU - Ikaga R AD - Center for Clinical Nutrition, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, 3-17 Senrioka Shinmachi, Settsu City, Osaka, Japan. Electronic address: reina.ikaga@otsuma.ac.jp. FAU - Matsumoto, Mai AU - Matsumoto M AD - Center for Nutritional Epidemiology and Policy, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, 3-17 Senrioka Shinmachi, Settsu City, Osaka 566-0002, Japan. Electronic address: m-matsumoto@nibn.go.jp. FAU - Chen, Kong Y AU - Chen KY AD - Diabetes, Endocrinology, and Obesity Branch, Intramural Research Program, National Institute of Diabetes and Digestive and Kidney Diseases, The National Institutes of Health, Bethesda, MD, USA. Electronic address: chenkong@niddk.nih.gov. FAU - Tanaka, Shigeho AU - Tanaka S AD - Faculty of Nutrition, Kagawa Nutrition University, Japan. Electronic address: tanaka.shigeho@eiyo.ac.jp. FAU - Higaki, Yasuki AU - Higaki Y AD - The Fukuoka University Institute for Physical Activity, Japan 8-19-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan. Electronic address: higaki@fukuoka-u.ac.jp. LA - eng PT - Journal Article DEP - 20260902 PL - England TA - Clin Nutr JT - Clinical nutrition (Edinburgh, Scotland) JID - 8309603 SB - IM OTO - NOTNLM OT - Chrono nutrition OT - Energy expenditure OT - Fat oxidation OT - Metabolic flexibility OT - Sleep metabolism COIS- Conflict of interest All authors declare no competing interests. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 18:59 PHST- 2025/07/24 00:00 [received] PHST- 2026/08/09 00:00 [revised] PHST- 2026/08/21 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 18:59 [entrez] AID - S0261-5614(26)00198-6 [pii] AID - 10.1016/j.clnu.2026.106771 [doi] PST - aheadofprint SO - Clin Nutr. 2026 Sep 2;67:106771. doi: 10.1016/j.clnu.2026.106771. PMID- 42945602 OWN - NLM STAT- Publisher LR - 20261027 IS - 1090-2414 (Electronic) IS - 0147-6513 (Linking) VI - 326 DP - 2026 Oct 27 TI - A cost-effective framework for drinking groundwater quality prediction based on explainable artificial intelligence. PG - 120965 LID - S0147-6513(26)01295-9 [pii] LID - 10.1016/j.ecoenv.2026.120965 [doi] AB - Urbanization and agricultural intensification are accelerating groundwater quality deterioration, yet most predictive studies focus primarily on accuracy while overlooking computational efficiency and economic feasibility, thereby constraining their applicability in sustainable groundwater management. To address these limitations, this study proposes a comprehensive framework for drinking water quality prediction that integrates multi-model prediction with Explainable Artificial Intelligence (XAI) techniques to achieve both efficiency and interpretability assessment. For validation, we collected 77 groundwater samples in a plain region in 2022. Hydrochemical analysis indicated that the HCO(3)-Ca type was the predominant hydrochemical facies, accounting for 54.55% of the samples. EWQI results showed that Na(+) (0.228), SO(4)(2)(-) (0.218), and NO(3)-N (0.188) exhibited higher weights than other parameters, highlighting the significant influence of the mirabilite-rich geological background on groundwater quality. 88.31% of groundwater samples were suitable for direct drinking, with 67.53% rated as excellent and 20.78% as good. In contrast, feature selection and importance analyses based on ElasticNet, SVM, XGBoost, and DNN models consistently identified NO(3)-N as the most influential parameter (SHAP contribution >50%), emphasizing the dominant role of anthropogenic inputs in groundwater quality deterioration. Among the evaluated models, the SVM demonstrated the best overall performance, achieving the highest predictive accuracy (R(2) = 0.997), the lowest uncertainty (mean standard uncertainty = 0.72), and an acceptable time cost. These findings provide a cost-effective and robust framework to support sustainable groundwater management and informed policymaking. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Wang, Hongji AU - Wang H AD - School of Economics, Xihua University, Chengdu, Sichuan, 610039, China. Electronic address: wanghj1007@163.com. FAU - Su, Yixuan AU - Su Y AD - School of Emergency Management, Xihua University, Chengdu, Sichuan, 610039, China. FAU - Fu, Yuxin AU - Fu Y AD - School of Emergency Management, Xihua University, Chengdu, Sichuan, 610039, China. FAU - Huang, Qirun AU - Huang Q AD - School of Emergency Management, Xihua University, Chengdu, Sichuan, 610039, China. FAU - He, Zhihao AU - He Z AD - School of Emergency Management, Xihua University, Chengdu, Sichuan, 610039, China. Electronic address: hezhihaohao@163.com. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Ecotoxicol Environ Saf JT - Ecotoxicology and environmental safety JID - 7805381 SB - IM OTO - NOTNLM OT - Computational efficiency OT - Drinking water quality index OT - Explainable artificial intelligence OT - Machine learning OT - Uncertainty COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 18:59 PHST- 2026/03/06 00:00 [received] PHST- 2026/09/13 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 18:59 [entrez] AID - S0147-6513(26)01295-9 [pii] AID - 10.1016/j.ecoenv.2026.120965 [doi] PST - aheadofprint SO - Ecotoxicol Environ Saf. 2026 Oct 27;326:120965. doi: 10.1016/j.ecoenv.2026.120965. PMID- 42945601 OWN - NLM STAT- Publisher LR - 20261027 IS - 1090-2414 (Electronic) IS - 0147-6513 (Linking) VI - 326 DP - 2026 Oct 27 TI - Bisphenol A induces ACSL4-associated ferro-aging-like urothelial injury: Implications for Hunner-type interstitial cystitis. PG - 120976 LID - S0147-6513(26)01306-0 [pii] LID - 10.1016/j.ecoenv.2026.120976 [doi] AB - Hunner-type interstitial cystitis (HIC) is an inflammatory subtype of interstitial cystitis/bladder pain syndrome marked by persistent urothelial injury and bladder dysfunction. Bisphenol A (BPA) can disturb redox and inflammatory homeostasis, but its relevance to HIC-associated urothelial vulnerability remains unclear. This study examined whether acyl-CoA synthetase long-chain family member 4 (ACSL4) contributes to BPA-associated ferro-aging-like urothelial injury. Among non-pregnant adult women, higher creatinine-corrected urinary BPA was associated with a phenotype predominantly characterized by urgency urinary incontinence (UUI), and the primary Mendelian randomization analysis suggested higher interstitial cystitis (IC) risk with genetically proxied BPA exposure. Integrated transcriptomic, BPA-target, and machine-learning analyses prioritized ACSL4 as a consistent HIC-associated candidate. HIC tissues showed higher ferro-aging-like transcriptional scores and ACSL4 expression; single-cell analyses further identified disease-associated urothelial cell-state remodeling, with higher ACSL4 expression and SenePy scores in HIC urothelial cells. Structure-based modeling suggested a plausible BPA-ACSL4 interaction, while cellular thermal shift assay (CETSA) indicated BPA-associated thermal stabilization of ACSL4. In primary human urothelial cells, BPA increased ACSL4 mRNA and protein expression, lipid peroxidation, iron accumulation, p21 expression, senescence-associated beta-galactosidase (SA-beta-Gal) positivity, inflammatory signaling, and barrier impairment, together with reduced Lamin B1 and ZO-1 expression and transepithelial electrical resistance (TEER). ACSL4 silencing and vitamin C attenuated these changes. In mice, PRGL493 and vitamin C each attenuated multiple BPA-induced bladder abnormalities. Together, these findings support an ACSL4-associated ferro-aging-like urothelial injury phenotype involving iron-lipid stress, senescence-associated changes, inflammation, and barrier impairment, with relevance to selected features of HIC. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Wu, Wenbo AU - Wu W AD - Department of Urology, The Affiliated Lianyungang Hospital of Xuzhou Medical University, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China. FAU - Xu, Yang AU - Xu Y AD - Department of Urology, Suqian Central People's Hospital, Suqian, Jiangsu, China. FAU - Lu, Zhongwen AU - Lu Z AD - Department of Urology, The Affiliated Lianyungang Hospital of Xuzhou Medical University, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China. FAU - Zhang, Yang AU - Zhang Y AD - Department of Urology, The Affiliated Lianyungang Hospital of Xuzhou Medical University, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China. FAU - Cai, Liyu AU - Cai L AD - Department of Urology, The Affiliated Lianyungang Hospital of Xuzhou Medical University, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China. FAU - Gao, Feng AU - Gao F AD - Department of Urology, The Affiliated Lianyungang Hospital of Xuzhou Medical University, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China. FAU - Niu, Zhuohan AU - Niu Z AD - Department of Urology, The Affiliated Lianyungang Hospital of Xuzhou Medical University, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China. FAU - Zong, Dayuan AU - Zong D AD - Central Health Center of Xukou Town, Wuzhong District, Suzhou, Jiangsu, China. FAU - Wang, Hui AU - Wang H AD - Department of Urology, The Affiliated Lianyungang Hospital of Xuzhou Medical University, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China. Electronic address: wanghui@xzhmu.edu.cn. FAU - Xue, Peng AU - Xue P AD - Department of Urology, The Affiliated Lianyungang Hospital of Xuzhou Medical University, The First People's Hospital of Lianyungang, Lianyungang, Jiangsu, China. Electronic address: xuepeng@njmu.edu.cn. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Ecotoxicol Environ Saf JT - Ecotoxicology and environmental safety JID - 7805381 SB - IM OTO - NOTNLM OT - ACSL4 OT - Bisphenol A OT - Ferro-aging OT - Interstitial cystitis OT - Urothelial injury OT - Vitamin C COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 18:59 PHST- 2026/07/15 00:00 [received] PHST- 2026/10/22 00:00 [revised] PHST- 2026/10/23 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 18:59 [entrez] AID - S0147-6513(26)01306-0 [pii] AID - 10.1016/j.ecoenv.2026.120976 [doi] PST - aheadofprint SO - Ecotoxicol Environ Saf. 2026 Oct 27;326:120976. doi: 10.1016/j.ecoenv.2026.120976. PMID- 42945599 OWN - NLM STAT- Publisher LR - 20261027 IS - 1090-2414 (Electronic) IS - 0147-6513 (Linking) VI - 326 DP - 2026 Oct 27 TI - Mitochondrial quality-control states in cadmium neurotoxicity: Adaptive clearance, disproportionate elimination and stalled flux. PG - 120982 LID - S0147-6513(26)01312-6 [pii] LID - 10.1016/j.ecoenv.2026.120982 [doi] AB - Cadmium (Cd) is a persistent environmental toxicant associated with neurological injury, but the role of mitophagy remains unresolved because increased pathway markers may indicate protective clearance, broad mitochondrial selection or incomplete downstream degradation. This review evaluates mitochondrial quality-control checkpoints in Cd-exposed neural systems, focusing on PINK1-Parkin, BNIP3L/NIX and autophagy-lysosome completion, and examines intervention evidence from puerarin, daidzein and ginsenoside Rg1. Three states are proposed as a conceptual synthesis: adaptive clearance, candidate disproportionate mitochondrial elimination and initiated but stalled flux. Protective PINK1- and BNIP3L-dependent activity has loss-of-function support, whereas selection-pathway engagement accompanied by mitochondrial loss is consistent with, but does not establish, disproportionate elimination because degradation and replacement have not been measured together. Cd also disrupts trafficking, autophagosome-lysosome fusion, lysosomal integrity and proteolysis, providing stronger evidence for incomplete bulk autophagic processing than for mitochondria-specific stalled flux. Among the compounds examined, puerarin has the broadest checkpoint coverage, largely from related cortical studies, linking reduced Cd burden and mitochondrial stress with pool preservation and improved bulk autophagic processing. Evidence for daidzein remains marker-limited, whereas Rg1 protects neuronal and glial endpoints without an established Cd-specific mitochondrial quality-control mechanism. This narrative, non-systematic synthesis is limited by potential selection bias, reliance on static markers, dominance of acute or high-dose exposure models, limited measurement of mitochondria-specific degradation and replacement, inadequate cell-type resolution and scarce independent replication. Resolving these states requires mitochondria-specific flux reporters, direct degradation and replacement indices, causal pathway perturbation and matched Cd and compound exposure. Therapeutic benefit is more likely to arise from restoring selective, proportionate and completed mitochondrial turnover balanced by replacement than from uniformly activating or suppressing mitophagy. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Li, Yuan AU - Li Y AD - The First Affiliated Hospital of Heilongjiang University of Chinese Medicine, Harbin, 150040, China. FAU - Ahmed, Nazir AU - Ahmed N AD - Guangxi Key Laboratory of Biology for Mango, College of Agriculture and Food Engineering, Baise University, Baise, 533000, China. Electronic address: nachachar@bsuc.cn. FAU - Li, Xiaofei AU - Li X AD - Department of Otolaryngology-Head and Neck Surgery, Shandong Provincial ENT Hospital, Shandong University, Jinan, 250022, China. FAU - Kubra, Khadijat Ul AU - Kubra KU AD - Shaheed Mohtarma Benazir Bhutto Medical University, Larkana, 77150, Pakistan. FAU - Rubab, Dua AU - Rubab D AD - Baqai Medical College, Baqai Medical University, Karachi, 75340, Pakistan. FAU - Lang, Tao AU - Lang T AD - School of Science and Technology, Hong Kong Metropolitan University, 999077, Hong Kong Special Administrative Region of China; Hong Kong Metropolitan University Shenzhen Research Institute, Shenzhen, 518031, China. Electronic address: tlang@hkmu.edu.hk. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Netherlands TA - Ecotoxicol Environ Saf JT - Ecotoxicology and environmental safety JID - 7805381 SB - IM OTO - NOTNLM OT - Autophagic flux OT - Cadmium OT - Mitochondrial depletion OT - Mitochondrial quality control OT - Mitophagy OT - Neurotoxicity OT - Puerarin COIS- Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 18:59 PHST- 2026/08/21 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/24 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 18:59 [entrez] AID - S0147-6513(26)01312-6 [pii] AID - 10.1016/j.ecoenv.2026.120982 [doi] PST - aheadofprint SO - Ecotoxicol Environ Saf. 2026 Oct 27;326:120982. doi: 10.1016/j.ecoenv.2026.120982. PMID- 42945596 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-376X (Electronic) IS - 1570-0232 (Linking) VI - 1285 DP - 2026 Oct 24 TI - An integrated UHPLC-Q-TOF-MS/MS strategy for comprehensive characterization of chemical constituents and in vivo metabolic profile of Bupi Yichang pills. PG - 125347 LID - S1570-0232(26)00436-8 [pii] LID - 10.1016/j.jchromb.2026.125347 [doi] AB - Bupi Yichang Pills (BYP) is a traditional Chinese medicine (TCM) prescription used for spleen-deficiency diarrhea and chronic colitis, yet its pharmacodynamic material basis remains unclear. This study employed UHPLC-Q-TOF-MS/MS to comprehensively characterize the chemical constituents of BYP in vitro and to identify prototype compounds and metabolites in rat plasma, urine, bile, feces, and tissues. In total, 183 compounds were identified in vitro, predominantly flavonoids, triterpenoid saponins, monoterpene glycosides, chromones, and coumarins. Based on this library, 93 prototype compounds and 98 metabolites were detected in vivo. Tissue distribution showed the largest numbers of compounds in the colon, ileum, and spleen. Flavonoids and monoterpene glycosides were the most frequently detected classes. Calycosin, paeoniflorin, and their metabolites were detected in the brain. These results indicate that the identified chemical classes are the major components detected in vivo in BYP and may serve as the candidate material basis for its efficacy, providing a scientific foundation for quality control marker selection, mechanism studies, and clinical application. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Han, Yun AU - Han Y AD - School of Traditional Chinese Medicine, Shandong Medical and Pharmaceutical University, Yantai, Shandong, 264003, PR China. Electronic address: hanyun.000@sdmpu.edu.cn. FAU - Li, Heng AU - Li H AD - Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation, Shenzhen Traditional Chinese Medicine Hospital, The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine, Shenzhen, 518033, PR China. FAU - Chen, Chen AU - Chen C AD - Hunan University of Chinese Medicine, 300 Xueshi Road, Changsha, Hunan, 410208, PR China. FAU - Xie, Yimin AU - Xie Y AD - State Key Laboratory of Traditional Chinese Medicine Syndrome, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510006, PR China. FAU - Xu, Kaixuan AU - Xu K AD - Institute of Innovative Drug Discovery and Development, Department of Chinese Medicines Analysis, China Pharmaceutical University, Nanjing, 210009, PR China. FAU - Zhu, Feng AU - Zhu F AD - Chongqing College of Traditional Chinese Medicine, Chongqing Bishan, Chongqing, 402760, PR China. FAU - Lin, Jinghong AU - Lin J AD - State Key Laboratory of Traditional Chinese Medicine Syndrome, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510006, PR China. FAU - Liang, Jian AU - Liang J AD - State Key Laboratory of Traditional Chinese Medicine Syndrome, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510006, PR China. Electronic address: ljyyq@gzucm.edu.cn. FAU - Wu, Jun AU - Wu J AD - Shandong College of Traditional Chinese Medicine, Yantai, Shandong, 264199, PR China. Electronic address: wujuntcm629@163.com. LA - eng PT - Journal Article DEP - 20261024 PL - Netherlands TA - J Chromatogr B Analyt Technol Biomed Life Sci JT - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JID - 101139554 SB - IM OTO - NOTNLM OT - BYP OT - Chemical constituents and metabolites analysis OT - Pharmacological substance basis OT - Tissue distribution OT - UHPLC-Q-TOF-MS/MS COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 18:59 PHST- 2026/07/25 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 18:59 [entrez] AID - S1570-0232(26)00436-8 [pii] AID - 10.1016/j.jchromb.2026.125347 [doi] PST - aheadofprint SO - J Chromatogr B Analyt Technol Biomed Life Sci. 2026 Oct 24;1285:125347. doi: 10.1016/j.jchromb.2026.125347. PMID- 42945593 OWN - NLM STAT- Publisher LR - 20261027 IS - 1878-562X (Electronic) IS - 1567-5394 (Linking) VI - 174 DP - 2026 Oct 21 TI - An electroactive RNA-cleaving DNAzyme based electrochemical biosensor for enzyme-free detection of microRNA. PG - 109483 LID - S1567-5394(26)00269-0 [pii] LID - 10.1016/j.bioelechem.2026.109483 [doi] AB - Cancer remains among the leading causes of mortality globally, where the detection of biomarkers is essential for early diagnosis and accurate prognosis. Among them, microRNA-21 (miR-21) is a clinically significant oncogenic biomarker that is dysregulated across various cancers, highlighting the need for rapid, sensitive, and cost-effective detection strategies. In this study, we introduced a unique electroactive RNA-cleaving DNAzyme (e-RCD)-based electrochemical biosensor for enzyme-free detection of miR-21. An Mg(2+) dependent 8-17 DNAzyme architecture was engineered to enable direct, target-specific recognition of miR-21. The target binding in the presence of Mg(2+) activates the e-RCD probe's catalytic cleavage mechanism, releasing the methylene blue-labelled fragment from the electrode surface and generating a concentration-dependent electrochemical signal. Under optimised sensing conditions, the biosensor demonstrated a broader linear detection range from 500 aM to 50 nM with a low limit of detection of 107 aM. Furthermore, the biosensor demonstrated higher selectivity against closely related miRNAs, and the target recovery studies in serum samples showed satisfactory analytical accuracy. These results support the platform's potential applicability to complex biological samples, and it can be extended to detect other miRNA targets. CI - Copyright (c) 2026 Elsevier B.V. All rights reserved. FAU - Karuppaiah, Gopi AU - Karuppaiah G AD - School of Integrative Engineering, Chung-Ang University, 84 Heuseok-ro, Dongjak-Gu, Seoul, 06974, Republic of Korea. FAU - Koyappayil, Aneesh AU - Koyappayil A AD - School of Integrative Engineering, Chung-Ang University, 84 Heuseok-ro, Dongjak-Gu, Seoul, 06974, Republic of Korea. FAU - Chavan, Sachin Ganpat AU - Chavan SG AD - School of Integrative Engineering, Chung-Ang University, 84 Heuseok-ro, Dongjak-Gu, Seoul, 06974, Republic of Korea. FAU - Go, Anna AU - Go A AD - School of Integrative Engineering, Chung-Ang University, 84 Heuseok-ro, Dongjak-Gu, Seoul, 06974, Republic of Korea. FAU - Lee, Min-Ho AU - Lee MH AD - School of Integrative Engineering, Chung-Ang University, 84 Heuseok-ro, Dongjak-Gu, Seoul, 06974, Republic of Korea. Electronic address: mhlee7@cau.ac.kr. LA - eng PT - Journal Article DEP - 20261021 PL - Netherlands TA - Bioelectrochemistry JT - Bioelectrochemistry (Amsterdam, Netherlands) JID - 100953583 SB - IM OTO - NOTNLM OT - Biomarker OT - Cancer detection OT - DNAzyme OT - Electrochemical biosensor OT - miRNA-21 COIS- Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 18:59 PHST- 2026/08/17 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 18:59 [entrez] AID - S1567-5394(26)00269-0 [pii] AID - 10.1016/j.bioelechem.2026.109483 [doi] PST - aheadofprint SO - Bioelectrochemistry. 2026 Oct 21;174:109483. doi: 10.1016/j.bioelechem.2026.109483. PMID- 42945591 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-4758 (Electronic) IS - 0955-3959 (Linking) VI - 158 DP - 2026 Oct 27 TI - Selective legalization: How medical cannabis regulation constitutes markets in Brazil and Colombia. PG - 105574 LID - S0955-3959(26)00426-3 [pii] LID - 10.1016/j.drugpo.2026.105574 [doi] AB - Medical cannabis reforms do not simply replace prohibition with regulation. They redraw the boundary of legality by recognizing some uses, products, and activities while others remain prohibited or are recognized within narrower legal limits. We call this process selective legalization and treat the legal boundary established by each reform as a contingent institutional outcome. Drawing on document analysis and a structured, focused comparison of Brazil and Colombia from 2014 to August 1, 2026, we examine what reforms legally recognize, the legal possibilities this recognition opens for economic activity, and the institutional routes through which recognized activities can be undertaken. Colombia authorized cultivation, processing, and foreign trade relatively early, a sequence associated with internationally oriented production. Brazil expanded individual-import and pharmacy-product routes while domestic cultivation lacked a general administrative authorization route in force at the study cutoff. These administrative routes relied on foreign products or cannabis-derived inputs and coexisted with judicially protected domestic production. The comparison shows that reforms sharing a medical purpose established different scopes of legal recognition. Institutional routes and unequal capacities shape the conditions under which these legal possibilities can be used. Effective participation and market configurations cannot be inferred from legal recognition alone. CI - Copyright (c) 2026 The Author(s). Published by Elsevier B.V. All rights reserved. FAU - Pereira, Paulo Jose Dos Reis AU - Pereira PJDR AD - Pontifical Catholic University of Sao Paulo (PUC-SP), Sao Paulo, Brazil. Electronic address: pjrpereira@pucsp.br. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Int J Drug Policy JT - The International journal on drug policy JID - 9014759 SB - IM OTO - NOTNLM OT - Brazil OT - Cannabis regulation OT - Colombia OT - Drug policy OT - Market formation OT - Medical cannabis OT - Political economy OT - Selective legalization COIS- Declaration of competing interest The author declares no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 18:59 PHST- 2026/05/21 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 18:59 [entrez] AID - S0955-3959(26)00426-3 [pii] AID - 10.1016/j.drugpo.2026.105574 [doi] PST - aheadofprint SO - Int J Drug Policy. 2026 Oct 27;158:105574. doi: 10.1016/j.drugpo.2026.105574. PMID- 42945588 OWN - NLM STAT- Publisher LR - 20261027 IS - 1476-928X (Electronic) IS - 1476-9271 (Linking) VI - 126 IP - Pt 2 DP - 2026 Oct 26 TI - Environmental impact on the tautomeric equilibrium of a pyrimidine-2,4-diol scaffold: In silico parametrization, structural transformation, and comparative docking analysis as multi-target directed ligands. PG - 109518 LID - S1476-9271(26)00645-6 [pii] LID - 10.1016/j.compbiolchem.2026.109518 [doi] AB - Resolving small-molecule tautomeric behavior through predictive frameworks is vital to prevent structural ambiguity during computer-aided drug design. We report an in silico parametrization and structural transformation analysis of a functionalized pyrimidine-2,4-diol scaffold as a Multi-Target Directed Ligand (MTDL). While spectroscopic analysis (MS, NMR) confirms imide-lactim form 3 in organic media, in silico profiling reveals a solvent-dependent shift to amide-lactam tautomer 4 (65-98%) across physiological pH ranges (3.0-7.0). Framework design benefits from paired theoretical screening and optimization, mirroring advanced biomaterials and multi-targeted aminopyrimidine protocols. Unlike rigid standalone pyrimidine cores lacking specialized protein-anchoring networks, our functionalized architecture provides an adaptive pharmacophoric environment compatible with sustainable synthesis matrices. Comparative automated molecular docking (AutoDock Vina, JAMDA) against human carbonic anhydrase (CAXII), CDK2, PptAB, and lactic acid bacterium LAB systems showed superior binding for tautomer 4 over form 3, yielding balanced multi-target affinity (DAR = 0.83-0.92). Computational ADME validation confirmed favorable drug-like profiles matching baseline bioavailability (0.55) with enhanced water solubility (965 mg/mL) and a lowered topological polar surface area (TPSA = 116.64 A2). Crucially, the automated preprocessing engine independently validated environmental impacts on tautomeric equilibrium, selectively prioritizing the keto form across three target pockets while preserving the structural diol state in the remaining cavity. Final binding topologies achieved high geometric convergence (RMSD down to 0.50 A). This study provides a rigorous rationale for environmental tautomer bioactivity, establishing a resource-saving predictive filtering tier that highlights the absolute necessity of automated aqueous parametrization and geometric validation prior to physical screening campaigns. CI - Copyright (c) 2026 Elsevier Ltd. All rights reserved. FAU - Farouk, M AU - Farouk M AD - Chemistry Department, Faculty of Science, Assiut University (recent New Valley University), Kharga Road, Assiut, New Valley, Egypt. Electronic address: mfa23@yahoo.com. FAU - Mahmoud, S AU - Mahmoud S AD - Faculty of pharmacy (PharmD Clinical Intern), Qena University, Qena, Egypt. FAU - Imran, A AU - Imran A AD - King Abdullah Institute for Nanotechnology, King Saud University, Riyadh, Saudi Arabia. FAU - Mahmoud, S AU - Mahmoud S AD - Faculty of pharmacy (3rd pharmD), South Valley National University, Qena, Egypt. LA - eng PT - Journal Article DEP - 20261026 PL - England TA - Comput Biol Chem JT - Computational biology and chemistry JID - 101157394 SB - IM OTO - NOTNLM OT - 3-substituted pyrimidine OT - In silico ADME OT - MTDLs OT - Molecular docking OT - Parametrization OT - Sonochemical synthesis OT - Uracil tautomerisation COIS- Declaration of Competing Interest The authors declare no competing interests. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 18:59 PHST- 2026/06/04 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/10/24 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 18:59 [entrez] AID - S1476-9271(26)00645-6 [pii] AID - 10.1016/j.compbiolchem.2026.109518 [doi] PST - aheadofprint SO - Comput Biol Chem. 2026 Oct 26;126(Pt 2):109518. doi: 10.1016/j.compbiolchem.2026.109518. PMID- 42945585 OWN - NLM STAT- Publisher LR - 20261027 IS - 2057-1976 (Electronic) IS - 2057-1976 (Linking) DP - 2026 Oct 27 TI - NCEL: Convolution-Enhanced Lightweight Neural Architecture for Robust EEG Signal Decoding in Noninvasive Brain-Computer Interfaces. LID - 10.1088/2057-1976/aeb9de [doi] AB - Electroencephalography (EEG) is a cornerstone of brain-computer interfaces (BCIs) and clinical neuro-diagnostics due to its non-invasiveness and high temporal resolution. However, robust EEG decoding remains challenging, particularly for resource-constrained deployments. While lightweight architectures like EEGNet offer favorable parameter efficiency, their reliance on fixed convolution kernels and restricted temporal receptive fields inherently limits their performance in complex, highly variable scenarios. To address these limitations, we propose a Neural Convolution-Enhanced Lightweight network (NCEL) that augments spatiotemporal EEG representations while preserving structural compactness. Specifically, a dynamic neural convolution module (NCM) is designed to learn input-dependent temporal filtering strategies for trial-to-trial variability and session-related distribution shifts.Furthermore, parallel multiscale temporal convolution branches are integrated to concurrently capture short-term local waveform transients and long-interval rhythmic dependencies. Finally, a lightweight channel-attention gating mechanism adaptively recalibrates intermediate representations, amplifying task-relevant neural responses while suppressing noise-dominated channels. Extensive evaluations on five benchmark datasets (BCIC IV-2a, BCIC IV-2b, ERN, SMR, and P300) demonstrate that NCEL achieves a mean classification accuracy of 82.75\% on BCIC IV-2b. Notably, NCEL achieves higher mean performance than the EEGNet reference values, with performance gains of 12.53 percentage points on the P300 dataset and 9.54 percentage points on BCIC IV-2b, while maintaining only $\sim$0.008M parameters. Additional subject-specific session-disjoint evaluations show comparable cross-session performance to EEGNet on BCIC IV-2a and a 1.80-percentage-point mean improvement on BCIC IV-2b. Overall, NCEL provides a parameter-efficient framework for resource-constrained EEG decoding, while broader cross-session generalization requires further validation. CI - (c) 2026 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved. FAU - Li, Yongwei AU - Li Y AD - College of Computer Science and Technology, Hengyang Normal University, Hengyang Normal University, Hengyang, 421008, China. FAU - Huang, Yongfei AU - Huang Y AUID- ORCID: 0000-0002-6721-2871 AD - College of Computer Science and Technology, Hengyang Normal University, Hengyang Normal University, Hengyang, 421008, China. FAU - Chen, Wenhui AU - Chen W AUID- ORCID: 0000-0003-1437-5551 AD - College of Computer Science and Technology, Hengyang Normal University, Hengyang Normal University, Hengyang, 421008, China. FAU - Jiao, Ge AU - Jiao G AD - School of Computer Science and Technology, Hengyang Normal University, Hengyang Normal University, Hengyang, 421008, China. FAU - Hu, Liangliang AU - Hu L AUID- ORCID: 0009-0001-3130-5064 AD - School of Computer Science and Technology, Chongqing University of Education, Chongjiao Road, Nanshan street, Nan'an District, Chongqing, Chongqing, 400065, China. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Biomed Phys Eng Express JT - Biomedical physics & engineering express JID - 101675002 SB - IM OTO - NOTNLM OT - Brain-Computer Interface (BCI) OT - Electroencephalography (EEG) OT - Lightweight Neural Network OT - Multiscale Temporal Modeling OT - Resource-Constrained EEG Decoding EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 18:53 PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 18:53 [entrez] AID - 10.1088/2057-1976/aeb9de [doi] PST - aheadofprint SO - Biomed Phys Eng Express. 2026 Oct 27. doi: 10.1088/2057-1976/aeb9de. PMID- 42945578 OWN - NLM STAT- Publisher LR - 20261027 IS - 1741-2552 (Electronic) IS - 1741-2552 (Linking) DP - 2026 Oct 27 TI - golgi: an open-source graphical platform for image-to-recruitment modeling of peripheral nerve stimulation. LID - 10.1088/1741-2552/aeba17 [doi] AB - OBJECTIVE: Computational models of peripheral nerve stimulation-coupling finite-element bioelectric fields to biophysical axon models-are essential for designing electrodes and waveforms for neuromodulation, but established open tools are code-first, assume substantial modeling expertise, and often depend on commercial finite-element solvers. We present golgi, an open-source platform that takes a peripheral nerve from image to stimulated fiber population. APPROACH: golgi integrates the full pipeline through a single graphical interface, with an equivalent scriptable Python API and command-line interface: image segmentation (or import of surfaces or masks), automated multi-region tetrahedral meshing, anisotropic finite-element solution of the extracellular field with explicit perineurium contact impedance, generation of realistic fiber populations and their three-dimensional trajectories (straight, or curved via a quasi-static streamline solver), and biophysical activation thresholds through interchangeable NEURON and a GPU-accelerated surrogate backend. MAIN RESULTS: We demonstrate golgi on extruded multifascicular swine and human cervical vagus nerves and on real three-dimensional, branching human and rabbit vagus nerves reconstructed from micro-computed tomography. It reproduces the physiological fiber-diameter recruitment order, quantifies fascicular selectivity and current steering with a multi-contact cuff, and resolves anatomically defined branches. Selectively engaging a vagal cardiac branch from a proximal cuff depends on anatomy: in the rabbit, whose cardiac fibers are predominantly small and whose superior cardiac branch forms a discrete tract, current steering isolates even its small cardiac (B-type) fibers; in the human only large myelinated fibers are separable, because the high thresholds of small cardiac fibers force currents that also recruit off-target fibers. SIGNIFICANCE: Every study exports as an integrity-hashed, self-contained bundle whose finite-element-to-recruitment provenance is verifiable byte-for-byte-a reproducibility guarantee absent from existing tools. By combining non-specialist usability, anatomical realism, and verifiable reproducibility, golgi lowers the barrier to in-silico peripheral nerve stimulation modeling. golgi is freely available as open-source software. CI - Creative Commons Attribution license. FAU - Lung, David AU - Lung D AUID- ORCID: 0009-0003-7748-2335 AD - Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Wahringer Gurtel 18-20, AKH-4L, Vienna, 1090, Austria. FAU - Jia, Yuting AU - Jia Y AUID- ORCID: 0009-0009-4111-3195 AD - Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Wahringergurtel 18-20, AKH-4L, Vienna, 1090, Austria. FAU - Blumer, Roland AU - Blumer R AUID- ORCID: 0000-0001-5048-4865 AD - Division of Anatomy, Medical University of Vienna, Wahringer Strasse 13, Vienna, 1090, Austria. FAU - Reissig, Lukas F F AU - Reissig LFF AUID- ORCID: 0000-0001-5833-8176 AD - Division of Anatomy, Medical University of Vienna, Wahringer Strasse 13, Vienna, 1090, Austria. FAU - Zopf, Lydia M AU - Zopf LM AUID- ORCID: 0000-0002-4895-0596 AD - Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstrasse 13, Vienna, 1200, Austria. FAU - Heimel, Patrick AU - Heimel P AD - Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstrasse 13, Vienna, 1200, Austria. FAU - Kraus, Christoph AU - Kraus C AD - Department of Psychiatry and Psychotherapy, Medical University of Vienna, Wahringer Gurtel 18-20, Vienna, 1090, Austria. FAU - Moro, Andrea AU - Moro A AUID- ORCID: 0009-0004-4123-4679 AD - Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Wahringer Gurtel 18-20, AKH-4L, Vienna, 1090, Austria. FAU - Fachino, Matteo AU - Fachino M AUID- ORCID: 0009-0006-5789-8722 AD - Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Wahringer Gurtel 18-20, AKH-4L, Vienna, 1090, Austria. FAU - Haberbusch, Max AU - Haberbusch M AUID- ORCID: 0000-0003-0712-4893 AD - Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Wahringer Gurtel 18-20, AKH-4L, Vienna, 1090, Austria. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - J Neural Eng JT - Journal of neural engineering JID - 101217933 SB - IM OTO - NOTNLM OT - current steering OT - fascicular selectivity OT - finite-element modeling OT - neuromodulation OT - open-source software OT - peripheral nerve stimulation OT - vagus nerve stimulation EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 18:52 PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 18:52 [entrez] AID - 10.1088/1741-2552/aeba17 [doi] PST - aheadofprint SO - J Neural Eng. 2026 Oct 27. doi: 10.1088/1741-2552/aeba17. PMID- 42945575 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-4030 (Electronic) IS - 1350-4533 (Linking) DP - 2026 Oct 27 TI - Mechanistic Investigation and Performance Evaluation of Ultrasonic Bone Drilling under Longitudinal-Torsional Coupled Vibration. LID - 10.1088/1873-4030/aeb9dc [doi] AB - Bone drilling is widely used in orthopedic and trauma surgery. However, conventional drilling generates high cutting forces and excessive heat, which may cause mechanical damage and increase the risk of thermal damage, thereby compromising hole quality. To address these limitations, a kinematic model of longitudinal-torsional ultrasonic vibration drilling (LTUVD) of cortical bone was developed. The model elucidates how periodic tool-bone contact and separation, micro-impacts, reverse shearing, dynamic changes in the effective rake angle, and complex cutting-edge trajectories collectively govern force generation and heat accumulation. Based on this model, three ultrasonic toolholders with different longitudinal-torsional coupling conditions were developed, and their effects on drilling force, temperature, and hole-wall surface quality were systematically evaluated. Compared with conventional drilling, LTUVD reduced drilling thrust by up to 55.9%, peak temperature by up to 17.8%, and hole-wall surface roughness by up to 42.4%. Among the tested longitudinal-to-torsional amplitude ratios (T/L), T/L = 0.425 produced the lowest drilling thrust and peak temperature. Surface roughness, however, varied non-monotonically and reached a minimum at T/L = 0.3, which also produced the most uniform hole-wall morphology. These findings indicate that the preferred T/L ratio depends on the performance criterion considered. The results provide a theoretical and experimental basis for selecting longitudinal-torsional vibration conditions and designing orthopedic drilling instruments with the potential to reduce bone damage. CI - (c) 2026 Institute of Physics and Engineering in Medicine. All rights, including for text and data mining, AI training, and similar technologies, are reserved. FAU - Deng, Naiwen AU - Deng N AD - Mechanical Engineering and Electronic Information, China University of Geosciences, No. 388, LUMO Road, Hongshan District, Wuhan 430077, China, Wuhan, 430074, China. FAU - Fang, Yu AU - Fang Y AD - Mechanical Engineering and Electronic Information, China University of Geosciences, No. 388, LUMO Road, Hongshan District, Wuhan 430077, China, Wuhan, 430074, China. FAU - Wang, Dong AU - Wang D AUID- ORCID: 0000-0003-1496-5308 AD - University of Exeter, Streatham Campus, Exeter, Devon, Exeter, EX4 4QJ, United Kingdom of Great Britain and Northern Ireland. FAU - Bai, Wei AU - Bai W AUID- ORCID: 0009-0004-0765-8531 AD - Mechanical Engineering and Electronic Information, China University of Geosciences, No. 388, LUMO Road, Hongshan District, Wuhan 430077, China, Wuhan, 430074, China. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Med Eng Phys JT - Medical engineering & physics JID - 9422753 SB - IM OTO - NOTNLM OT - Drilling quality OT - Longitudinal-torsional coupled vibration OT - Mechanistic analysis OT - Thermo-mechanical damage OT - Ultrasonic bone drilling EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 18:52 PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 18:52 [entrez] AID - 10.1088/1873-4030/aeb9dc [doi] PST - aheadofprint SO - Med Eng Phys. 2026 Oct 27. doi: 10.1088/1873-4030/aeb9dc. PMID- 42945546 OWN - NLM STAT- Publisher LR - 20261027 IS - 2168-6602 (Electronic) IS - 0890-1171 (Linking) DP - 2026 Oct 27 TI - The Burden of Depressive Symptoms Associated With Gun Violence Exposure in the US Relative to Other Conditions. PG - 8901171261502222 LID - 10.1177/08901171261502222 [doi] AB - PurposeWe compare the burden of depressive symptoms associated with gun violence exposure (GVE) to the burden of other conditions experienced by the US adult population to display the magnitude of opportunity to reduce emotional distress by reducing GVE.Design, Setting, and SampleSurvey representative of the US adult population.MeasuresRespondent reports of cumulative frequency of gun violence exposure and PHQ-9 depressive symptom scores.AnalysisWe estimate the excess PHQ-9 points associated with GVE and convert them into disability-adjusted life years (DALYs) using disability weights from the Global Burden of Disease study. We then compare these DALYs to DALYs attributed to selected conditions that burden the US population.ResultsWe estimated that the excess PHQ-9 points associated with GVE represent 3.15 million DALYs. This burden was approximately one-third that of ischemic heart disease and was slightly less than the burden of Alzheimer's disease, chronic kidney disease, and lung cancer. It was almost twice the burden of colon cancer, firearm deaths, and breast cancer. When combined, the burden of fatal firearm injury together with the burden of depressive symptoms associated with GVE would only be less than the burdens of ischemic heart disease, drug use disorders, falls, and diabetes mellitus.ConclusionImplementing interventions that reduce the burden of GVE might have a significant impact on both individual and community well-being. FAU - Kottke, Thomas E AU - Kottke TE AUID- ORCID: 0000-0002-0349-9404 AD - Research, HealthPartners Institute, Minneapolis, MN, USA. RINGGOLD: 51441 FAU - Dehmer, Steven P AU - Dehmer SP AUID- ORCID: 0000-0003-3508-9239 AD - Research, HealthPartners Institute, Minneapolis, MN, USA. RINGGOLD: 51441 FAU - Hooker, Stephanie A AU - Hooker SA AUID- ORCID: 0000-0003-4222-7046 AD - Research, HealthPartners Institute, Minneapolis, MN, USA. RINGGOLD: 51441 FAU - Arena, Ross AU - Arena R AUID- ORCID: 0000-0002-6675-1996 AD - Institute of Government and Public Affairs, University of Illinois Chicago, Chicago, IL, USA. RINGGOLD: 14681 AD - Administration, HealthPartners, Minneapolis, MN, USA. FAU - Pronk, Nicolaas P AU - Pronk NP AUID- ORCID: 0000-0002-5825-6733 AD - Research, HealthPartners Institute, Minneapolis, MN, USA. RINGGOLD: 51441 AD - Administration, HealthPartners, Minneapolis, MN, USA. FAU - Semenza, Daniel C AU - Semenza DC AUID- ORCID: 0000-0002-3843-9559 AD - Research, New Jersey Gun Violence Research Center, Rutgers University, Piscataway, NJ, USA. RINGGOLD: 583540 AD - Department of Urban-Global Public Health, Rutgers University, Piscataway, NJ, USA. RINGGOLD: 583540 LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Am J Health Promot JT - American journal of health promotion : AJHP JID - 8701680 SB - IM OTO - NOTNLM OT - burden of disease OT - depressive symptoms OT - gun violence OT - health promotion OT - patient health Questionnaire-9 EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 18:33 PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 18:33 [entrez] AID - 10.1177/08901171261502222 [doi] PST - aheadofprint SO - Am J Health Promot. 2026 Oct 27:8901171261502222. doi: 10.1177/08901171261502222. PMID- 42945543 OWN - NLM STAT- Publisher LR - 20261027 IS - 1476-1645 (Electronic) IS - 0002-9637 (Linking) DP - 2026 Oct 27 TI - Associations between Inflammation-Adjusted Vitamin A Status and Schistosoma japonicum Infection Intensity among Children and Adolescents in The Philippines: A Cross-Sectional Study. LID - tpmd260043 [pii] LID - 10.4269/ajtmh.26-0043 [doi] AB - Vitamin A deficiency impairs immune function against parasitic pathogens, which may be linked to schistosomiasis morbidity in children. Adjusting measures of vitamin A status to account for inflammation is necessary to estimate vitamin A status during periods of infection. A secondary analysis of a treatment-reinfection study of children and adolescents with Schistosoma japonicum infection from the Philippines was performed in the present study. Measurements included S. japonicum infection intensity via Kato-Katz (eggs per gram of stool [EPG]), retinol-binding protein (RBP), C-reactive protein (CRP), and alpha-1-acid glycoprotein (AGP). The Thurnham Correction Factor (TCF) inflammation-adjustment method was applied to RBP using measures of CRP and AGP. A sample of n = 40 participants aged 8 to 18 years was randomly selected on the basis of S. japonicum EPG distribution. After applying the TCF method, 14 participants (35.9%) were vitamin A-deficient (RBP <0.7 micromol/L). Using multivariate regression models adjusting for sex, vitamin A deficiency was associated with higher S. japonicum EPG at baseline (P = 0.02). Baseline vitamin A status was not associated with S. japonicum infection intensity (EPG) at subsequent visits over 18 months. This association between vitamin A deficiency and increased schistosomiasis egg burden agrees with findings from animal studies and enhances understanding of nutritional modulation of infection, specific to schistosomiasis. FAU - Colt, Susannah AU - Colt S AD - Center for International Health Research, Rhode Island Hospital, Providence, Rhode Island, USA. AD - Department of Pediatrics, The Warren Alpert Medical School of Brown University, Providence, Rhode Island, USA. FAU - Wu, Hannah W AU - Wu HW AD - Center for International Health Research, Rhode Island Hospital, Providence, Rhode Island, USA. AD - Department of Pediatrics, The Warren Alpert Medical School of Brown University, Providence, Rhode Island, USA. FAU - Jiz, Mario AU - Jiz M AD - Department of Health, Research Institute for Tropical Medicine, Manila, Philippines. FAU - Acosta, Luz P AU - Acosta LP AD - Department of Immunology, Research Institute of Tropical Medicine, Manila, the Philippines. FAU - Jarilla, Blanca R AU - Jarilla BR AD - Department of Health, Research Institute for Tropical Medicine, Manila, Philippines. FAU - Duggan, Christopher P AU - Duggan CP AD - Center for Nutrition, Division of Gastroenterology, Hepatology, and Nutrition, and the Margaret C. Ryan Program in Global Health, Boston Children's Hospital, Boston, Massachusetts, USA. AD - Departments of Nutrition and Global Health and Population, Harvard T.H. Chan School of Public Health, Boston, Massachusetts, USA. FAU - Kurtis, Jonathan D AU - Kurtis JD AD - Center for International Health Research, Rhode Island Hospital, Providence, Rhode Island, USA. AD - Department of Pathology and Laboratory Medicine, The Warren Alpert Medical School of Brown University, Providence, Rhode Island, USA. FAU - Friedman, Jennifer F AU - Friedman JF AD - Center for International Health Research, Rhode Island Hospital, Providence, Rhode Island, USA. AD - Department of Pediatrics, The Warren Alpert Medical School of Brown University, Providence, Rhode Island, USA. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Am J Trop Med Hyg JT - The American journal of tropical medicine and hygiene JID - 0370507 SB - IM EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:28 CRDT- 2026/10/27 18:33 PHST- 2026/01/27 00:00 [received] PHST- 2026/07/15 00:00 [accepted] PHST- 2026/10/27 23:28 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 18:33 [entrez] AID - tpmd260043 [pii] AID - 10.4269/ajtmh.26-0043 [doi] PST - aheadofprint SO - Am J Trop Med Hyg. 2026 Oct 27:tpmd260043. doi: 10.4269/ajtmh.26-0043. PMID- 42945536 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1665-1731 (Electronic) IS - 1405-9940 (Print) IS - 1665-1731 (Linking) VI - 96 IP - Supl 4 DP - 2026 TI - Inter-American consensus on hypertrophic cardiomyopathy. PG - 1-60 LID - 10.24875/ACM.26000187 [doi] AB - BACKGROUND: Hypertrophic cardiomyopathy (HCM) is the most common inherited cardiomyopathy and poses major diagnostic and therapeutic challenges in Latin America, owing to heterogeneity in resource availability, access to advanced technologies, and the organization of health systems. OBJECTIVES: To develop the first Inter-American Consensus on HCM, in order to standardize the care of these patients tailored to the regional context. METHODS: The Inter-American Society of Cardiology (SIAC) convened a committee of experts from different countries across the Americas. A systematic review of the evidence was performed, followed by the development of recommendations, which were validated using a formal Delphi methodology; consensus was defined as agreement among more than 80% of participants. RESULTS: The consensus provides a comprehensive approach to the evaluation and management of HCM. It covers epidemiology, pathophysiology, multimodality diagnostic tools, genetics and family counseling, phenocopies, pharmacological and invasive treatment, associated complications, sudden cardiac death risk assessment, special populations (pediatric and pregnant patients), physical exercise, and follow-up strategies. In addition, it includes specific recommendations aimed at facilitating the implementation of these across Latin America. CONCLUSIONS: The Inter-American Consensus on HCM provides recommendations for the diagnosis and treatment of this condition. It integrates the best available evidence with regional clinical experience and takes into account the epidemiological, economic, and healthcare characteristics of the region. It constitutes a reference framework for standardizing the care of patients with HCM and their families throughout Latin America. CI - Copyright: (c) 2026 Permanyer. FAU - Parodi, Josefina B AU - Parodi JB AD - Departamento de Cardiologia, Sanatorio Anchorena San Martin, San Martin, Buenos Aires, Argentina. FAU - Scatularo, Cristhian E AU - Scatularo CE AD - Departamento de Cardiologia, Sanatorio de la Trinidad de Palermo, Buenos Aires, Argentina. FAU - Berrios-Barcenas, Enrique A AU - Berrios-Barcenas EA AD - Departamento de Consulta Externa, Instituto Nacional de Cardiologia Ignacio Chavez, Ciudad de Mexico, Mexico. FAU - Leon, Juan D Lopez-Ponce de AU - Leon JDL AD - Departamento de Cardiologia, Fundacion Valle del Lili, Cali, Colombia. FAU - Arenaza, Diego Perez de AU - Arenaza DP AD - Servicio de Cardiologia, Hospital Italiano de Buenos Aires, Buenos Aires, Argentina. FAU - Gomez, Luis E AU - Gomez LE AD - Division de Cardiologia, Hospital General de Agudos Juan A. Fernandez, Buenos Aires, Argentina. FAU - Costabel, Juan P AU - Costabel JP AD - Unidad Coronaria, Instituto Cardiovascular de Buenos Aires, Buenos Aires, Argentina. FAU - Fernandes, Fabio AU - Fernandes F AD - Grupo de Cardiomiopatias, Instituto do Coracao (InCor), Sao Paulo, Brasil. FAU - Saldarriaga, Clara AU - Saldarriaga C AD - Clinica CardioVID, Universidad Pontificia Bolivariana, Medellin, Colombia. FAU - Rossel, Victor AU - Rossel V AD - Departamento de Cardiologia, Hospital del Salvador, Santiago de Chile, Chile. FAU - Rochitte, Carlos E AU - Rochitte CE AD - Instituto do Coracao (InCor), Sao Paulo, Brasil. FAU - Stein, Ricardo AU - Stein R AD - Faculdade de Medicina, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brasil. FAU - Concepcion-Chacon, Roberto AU - Concepcion-Chacon R AD - Unidad de Insuficiencia Cardiaca, Hospital DIPRECA, Santiago de Chile, Chile. FAU - Parma-Romanelli, Gabriel AU - Parma-Romanelli G AD - Departamento de Cardiologia, Hospital Evangelico, Montevideo, Uruguay. FAU - Gonzalez-Carta, Karina AU - Gonzalez-Carta K AD - Departamento de Cirugia Ortopedica, Mayo Clinic, Rochester, Minnesota, EE.UU. FAU - Blanco-Sobrino, Susana AU - Blanco-Sobrino S AD - Servicio de Cardiologia, Instituto de Clinicas y Urologia Tamanaco, Caracas, Venezuela. FAU - Sierra-Lara-Martinez, Daniel AU - Sierra-Lara-Martinez D AD - Urgencias y Unidad de Cuidados Criticos Coronarios, Instituto Nacional de Cardiologia Ignacio Chavez, Ciudad de Mexico, Mexico. FAU - Leoni, Franco AU - Leoni F AD - Medica Uruguaya (MUCAM), Montevideo, Uruguay. FAU - Jalil, Jorge AU - Jalil J AD - Division de Enfermedades Cardiovasculares, Pontificia Universidad Catolica de Chile, Santiago, Chile. FAU - Delgado, Yamiris AU - Delgado Y AD - Servicio de Cardiologia, Centro Medico Real, Santo Domingo, Republica Dominicana. FAU - Chango, Diego X AU - Chango DX AD - Servicio de Cardiologia, Clinica Santa Ana, Cuenca, Ecuador. FAU - Posada-Martinez, Liliana AU - Posada-Martinez L AD - Departamento de Ecocardiografia, Instituto Nacional de Cardiologia Ignacio Chavez, Ciudad de Mexico, Mexico. FAU - Rodriguez-Amaguay, Landy AU - Rodriguez-Amaguay L AD - Servicio de Imagenes Cardiovasculares, Centro Ecuatoriano de Diagnostico Cardiovascular, Guayaquil, Ecuador. FAU - Guaman-Valdivieso, Carlos AU - Guaman-Valdivieso C AD - Novaclinica Santa Cecilia, Quito, Ecuador. FAU - Rendon-Giraldo, Jairo A AU - Rendon-Giraldo JA AD - Departamento de Cardiologia, Hospital San Vicente Fundacion, Medellin, Colombia. FAU - Arevalos, Victor AU - Arevalos V AD - Departamento de Cardiologia, Hospital Central del Instituto de Prevision Social, Asuncion, Paraguay. FAU - Marquez, Ingrid AU - Marquez I AD - Departamento de Cardiologia, Hospital del Este Dr. Domingo Luciani, Caracas, Venezuela. FAU - Cantora, Florencia B AU - Cantora FB AD - Servicio de Cardiologia, Hospital Italiano de Buenos Aires, Buenos Aires, Argentina. FAU - Estofan, Mariano D AU - Estofan MD AD - Sanatorio 9 de Julio, San Miguel de Tucuman, Argentina. FAU - Cardenas-Reyes, Ivonne AU - Cardenas-Reyes I AD - Cardiologia Pediatrica, Health in Code, Valencia, Espana. FAU - Brogger, Noel AU - Brogger N AD - Cardiologia Pediatrica, Health in Code, Valencia, Espana. FAU - Bejarano, Maria C AU - Bejarano MC AD - Centro de Investigaciones Clinicas (CIC), Fundacion Valle del Lili, Cali, Colombia. FAU - Lara-Teran, Joffre AU - Lara-Teran J AD - Servicio de Cardiologia, Hospital Juan Tanca Marengo, Guayaquil, Ecuador. FAU - Vaca-Valverde, Ignacio AU - Vaca-Valverde I AD - Cardiovida, Santa Cruz de la Sierra, Bolivia. FAU - Olivares-Asencio, Carlos AU - Olivares-Asencio C AD - Departamento de Cardiologia, Hospital Hernan Henriquez Aravena, Temuco, Chile. FAU - Cura-Hochbaum, Leandro AU - Cura-Hochbaum L AD - Cardiocentro, Asociacion Espanola, Montevideo, Uruguay. FAU - Morejon-Barragan, Paola A AU - Morejon-Barragan PA AD - Clinica Guayaquil, Guayaquil, Ecuador. FAU - Fava, Agostina M AU - Fava AM AD - Cleveland Clinic, Cleveland, Ohio, EE.UU. FAU - Miranda-Arboleda, Andres F AU - Miranda-Arboleda AF AD - Instituto de Cardiologia, Universidad de Ottawa, Ottawa, Canada. FAU - Marquez-Murillo, Manlio F AU - Marquez-Murillo MF AD - Departamento de Cardiologia, Centro Medico ABC, Ciudad de Mexico, Mexico. FAU - Karabut, Eric AU - Karabut E AD - Departamento de Cardiologia, Hospital Punta Pacifica, Ciudad de Panama, Panama. FAU - Berni-Betancourt, Ana C AU - Berni-Betancourt AC AD - Departamento de Cardiologia, Hospital Angeles Pedregal, Ciudad de Mexico, Mexico. FAU - Alba, Ana C AU - Alba AC AD - Peter Munk Cardiac Centre, University Health Network, Toronto, Canada. FAU - Rodriguez-Gonzalez, Maria J AU - Rodriguez-Gonzalez MJ AD - Fundacion Cardioinfantil, Bogota, Colombia. FAU - Saenz-Tejeira, Maria M AU - Saenz-Tejeira MM AD - Servicio de Cardiologia Pediatrica, Hospital Italiano de Buenos Aires, Buenos Aires, Argentina. FAU - Oca, Katihurca Almonte-Montes de AU - Oca KA AD - Asociacion Instituto Dominicano de Cardiologia, Santo Domingo, Republica Dominicana. FAU - Cueto, Rosa N AU - Cueto RN AD - Departamento de Cardiologia, Hospital Regional Dr. Antonio Musa, San Pedro de Macoris, Republica Dominicana. FAU - Rodriguez-Israel, Melissa AU - Rodriguez-Israel M AD - Departamento de Cardiologia, Hospital San Vicente de Paul, Heredia, Costa Rica. FAU - Torterolo-Herrera, Alicia G AU - Torterolo-Herrera AG AD - Departamento de Cardiologia, Hospital de Clinicas, Montevideo, Uruguay. FAU - Villacreses, Vicente AU - Villacreses V AD - Departamento de Cardiologia, Manahospital, Portoviejo, Ecuador. FAU - Restrepo, Gustavo AU - Restrepo G AD - Clinica ANUA Las Americas, Medellin, Colombia. FAU - Sosa-Liprandi, Maria I AU - Sosa-Liprandi MI AD - Departamento de Cardiologia, Sanatorio Guemes, Buenos Aires, Argentina. FAU - Cingolani, Eugenio AU - Cingolani E AD - Departamento de Cardiologia, Centro Medico Cedars-Sinai, Universidad de California, Los Angeles, California, EE.UU. FAU - Baratta, Sergio AU - Baratta S AD - Servicio de Cardiologia, Hospital Universitario Austral, Buenos Aires, Argentina. FAU - Lopez-Santi, Ricardo AU - Lopez-Santi R AD - Departamento de Cardiologia, Hospital Italiano de La Plata, La Plata, Argentina. FAU - Munera-Echeverri, Ana AU - Munera-Echeverri A AD - Departamento de Cardiologia, Clinica Rosario Tesoro-Cardioestudio, Medellin, Colombia. FAU - Baranchuk, Adrian AU - Baranchuk A AD - Facultad de Medicina, Universidad Abierta Interamericana (UAI), Buenos Aires, Argentina. LA - eng PT - Consensus Statement PT - Journal Article PT - Systematic Review TT - Consenso interamericano de miocardiopatia hipertrofica. PL - Mexico TA - Arch Cardiol Mex JT - Archivos de cardiologia de Mexico JID - 101126728 SB - IM MH - Humans MH - *Cardiomyopathy, Hypertrophic/therapy/diagnosis/physiopathology/epidemiology MH - Latin America PMC - PMC13713040 OTO - NOTNLM OT - America Latina OT - Cardiac myosin inhibitors OT - Consenso OT - Consensus OT - Genetic testing OT - Hypertrophic cardiomyopathy OT - Inhibidores de la miosina cardiaca OT - Latin America OT - Miocardiopatia hipertrofica OT - Muerte subita cardiaca OT - Pruebas geneticas OT - Sudden cardiac death COIS- Los autores declaran no tener conflictos de intereses relacionados con el contenido de este manuscrito. EDAT- 2026/10/27 23:28 MHDA- 2026/10/27 23:29 PMCR- 2026/01/01 CRDT- 2026/10/27 17:13 PHST- 2026/10/27 23:29 [medline] PHST- 2026/10/27 23:28 [pubmed] PHST- 2026/10/27 17:13 [entrez] PHST- 2026/01/01 00:00 [pmc-release] AID - j96/Supl 4/1 [pii] AID - 12352AMEX261-ACM-96-1 [pii] AID - 10.24875/ACM.26000187 [doi] PST - ppublish SO - Arch Cardiol Mex. 2026;96(Supl 4):1-60. doi: 10.24875/ACM.26000187. PMID- 42945501 OWN - NLM STAT- Publisher LR - 20261027 IS - 1873-233X (Electronic) IS - 0029-7844 (Linking) DP - 2026 Oct 27 TI - Antidepressant Discontinuation During Pregnancy and Risk of Mental Health-Related Emergencies. LID - 10.1097/AOG.0000000000006455 [doi] AB - OBJECTIVE: To evaluate contemporary patterns of antidepressant discontinuation during pregnancy and associated maternal mental health outcomes, including behavioral health-related emergency department (ED) visits, among patients with preexisting depression or anxiety. METHODS: This was a retrospective cohort study of a state-based private insurance claims database in Pennsylvania. Patients who delivered between January 1, 2023, and December 31, 2024, with a diagnosis of depression or anxiety before pregnancy and an active prescription for a selective serotonin reuptake inhibitor (SSRI) or serotonin-norepinephrine reuptake inhibitor (SNRI) in the 3 months before pregnancy were included. The exposure was antidepressant continuation compared with discontinuation during pregnancy, defined as no prescription fills or a gap of more than 60 days. The primary outcome was behavioral health-related ED visits during pregnancy and the postpartum period. Behavioral health-related ED visit rates were compared between patients who continued and those who discontinued antidepressants with appropriate bivariate statistical tests. RESULTS: Among 3,983 patients with depression or anxiety, 1,462 (36.7%) entered pregnancy with an active SSRI or SNRI prescription. Of these, 260 (17.8%) had no medication fills during pregnancy, and 945 (64.6%) had a gap of more than 60 days. Among patients with a treatment gap of more than 60 days, discontinuation occurred most commonly in the first trimester (44.9%) followed by the third trimester (30.3%). Patients who continued and those who discontinued treatment had similar rates of outpatient and ED mental health visits before pregnancy. However, discontinuation was associated with a higher risk of mental health-related emergency visits during pregnancy, with peaks in the second month (58 vs 37/1,000, P=.027) and the ninth month (59 vs 29/1,000, P<.01) of pregnancy. This increased risk persisted postpartum, with higher rates of mental health-related ED visits within 8 months after delivery among those who discontinued treatment during pregnancy (32 vs 21/1,000, P=.02). CONCLUSION: In this cohort of privately insured patients with preexisting depression or anxiety, antidepressant discontinuation during pregnancy was common and was associated with increased risk of mental health-related ED visits. These findings underscore the need for clinical and policy strategies to support continuation of evidence-based treatment for perinatal mental health conditions and to address preventable contributors to maternal morbidity and mortality. CI - Copyright (c) 2026 by the American College of Obstetricians & Gynecologists. Published by Wolters Kluwer Health, LLC. All rights reserved. FAU - Zafman, Kelly B AU - Zafman KB AD - Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, and the Department of Psychiatry, Perelman School of Medicine, University of Pennsylvania, and Independence Blue Cross, Philadelphia, Pennsylvania. FAU - Zhu, Yifan AU - Zhu Y FAU - Kornfield, Sara L AU - Kornfield SL FAU - Smith-McLallen, Aaron AU - Smith-McLallen A FAU - Srinivas, Sindhu K AU - Srinivas SK LA - eng GR - AMETHIST@PENN/ PT - Journal Article DEP - 20261027 PL - United States TA - Obstet Gynecol JT - Obstetrics and gynecology JID - 0401101 SB - IM COIS- Financial Disclosure Dr. Zafman reports serving as a consultant to Phia Health and receiving equity compensation. Phia Health had no role in the design, conduct, analysis, or reporting of this study. The other authors did not report any potential conflicts of interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 16:03 PHST- 2026/06/05 00:00 [received] PHST- 2026/08/13 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 16:03 [entrez] AID - 00006250-990000000-01628 [pii] AID - 10.1097/AOG.0000000000006455 [doi] PST - aheadofprint SO - Obstet Gynecol. 2026 Oct 27. doi: 10.1097/AOG.0000000000006455. PMID- 42945480 OWN - NLM STAT- Publisher LR - 20261027 IS - 0894-0282 (Print) IS - 0894-0282 (Linking) DP - 2026 Oct 27 TI - The Integrated Virulence Network of Colletotrichum scovillei: Effector-Signaling Coordination in an Emerging Anthracnose Pathogen. LID - 10.1094/MPMI-08-26-0090-IRW [doi] AB - Colletotrichum scovillei is an important and geographically expanding anthracnose pathogen of pepper (Capsicum spp.) that causes serious pre- and post-harvest losses. This hemibiotrophic fungus employs a multilayered infection strategy, yet an integrated framework connecting its molecular mechanisms to disease development has been lacking. This review synthesizes current evidence to propose an integrated virulence network for C. scovillei. Conserved intracellular signaling pathways, including mitogen-activated protein kinase (MAPK), cyclic AMP (cAMP), calcium/calcineurin, cell-wall integrity, and small-GTPase modules, regulate key morphological transitions. Available genetic studies also connect selected regulators to host-defense suppression, while comparative genomic analyses identify strain-level differences in predicted secreted proteins and candidate virulence-associated regions. However, the identities, host targets, and stage-specific deployment of most C. scovillei effectors remain unresolved. We therefore distinguish direct evidence from the C. scovillei-pepper system from mechanisms inferred from other Colletotrichum species and position biotrophy, extended quiescence, ripening-associated reactivation, and necrotrophy within a testable framework. We further examine pepper resistance and management limitations and identify experiments needed to translate this framework into durable control strategies. FAU - Vanessa, Nibigira AU - Vanessa N AD - Chinese Academy of Agricultural Sciences Institute of Vegetables and Flowers, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China; vanessanibigiravanessa@gmail.com. FAU - Zhang, Zhenghai AU - Zhang Z AD - Chinese Academy of Agricultural Sciences Institute of Vegetables and Flowers, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China; zhangzhenghai@caas.cn. FAU - Cao, Yacong AU - Cao Y AD - Chinese Academy of Agricultural Sciences Institute of Vegetables and Flowers, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China; caoyacong@caas.cn. FAU - Wu, Huamao AU - Wu H AD - Chinese Academy of Agricultural Sciences Institute of Vegetables and Flowers, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China; wuhuamao@caas.cn. FAU - Yu, Hailong AU - Yu H AD - Chinese Academy of Agricultural Sciences Institute of Vegetables and Flowers, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China; yuhailong@caas.cn. FAU - Zhao, Fangqi AU - Zhao F AD - Chinese Academy of Agricultural Sciences Institute of Vegetables and Flowers, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China; 13171190713@163.com. FAU - Thandar, Khin AU - Thandar K AD - Chinese Academy of Agricultural Sciences Institute of Vegetables and Flowers, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China; kthandar85@gmail.com. FAU - Albert, Iribagiza AU - Albert I AD - University of Burundi, Research Center in Animal, Crop and Environmental Sciences (CRAVE), Bujumbura, Bujumbura, Burundi. AD - Chinese Academy of Agricultural Sciences Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Genetic molecular breedings, Lanzhou, Gansu, China; albert.iribagiza@ub.edu.bi. FAU - Su, Qiang AU - Su Q AD - Chinese Academy of Agricultural Sciences, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, Beijing, China; suqiang@caas.cn. FAU - Wang, Lihao AU - Wang L AD - Chinese Academy of Agricultural Sciences Institute of Vegetables and Flowers, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China; wanglihao@caas.cn. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Mol Plant Microbe Interact JT - Molecular plant-microbe interactions : MPMI JID - 9107902 SB - IM EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 15:43 PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 15:43 [entrez] AID - 10.1094/MPMI-08-26-0090-IRW [doi] PST - aheadofprint SO - Mol Plant Microbe Interact. 2026 Oct 27. doi: 10.1094/MPMI-08-26-0090-IRW. PMID- 42945476 OWN - NLM STAT- Publisher LR - 20261027 IS - 1460-2156 (Electronic) IS - 0006-8950 (Linking) DP - 2026 Oct 27 TI - KCNH1 variants associated with neurodevelopmental disorders exhibit prominent gain of function. LID - awag373 [pii] LID - 10.1093/brain/awag373 [doi] AB - Pathogenic variants in KCNH1, encoding the human ether-a-go-go voltage-gated potassium channel (EAG1, KV10.1), are associated with the neurodevelopmental disorders Temple-Baraitser syndrome (TBS), Zimmermann-Laband syndrome (ZLS), epileptic encephalopathy, and other undefined neurodevelopmental disorders. Investigating the functional consequences of KCNH1 variants can provide information about molecular and cellular pathophysiological mechanisms and help guide therapeutic directions. In this study, we determined the functional properties of 26 disease-associated KCNH1 variants including three recurrent and six that were previously unpublished. Findings were compared to the wildtype (WT) channel along with six rare population variants. Our approach used automated patch clamp recording of heterologously expressed KCNH1 variants in both the homozygous state (variant alone) and the clinically-relevant heterozygous state (variant co-expressed with WT channels). We also examined the impact of alternative splice isoforms on functional consequences of specific variants. We demonstrated that the majority of disease-associated KCNH1 variants exhibit features consistent with gain-of-function including hyperpolarized voltage-dependence of activation, accelerated activation kinetics, or both that promote hyperpolarization of the resting membrane potential. By contrast, one unique variant discovered previously in an autism cohort had a depolarized voltage-dependence of activation and was associated with a depolarized resting potential in cells. Pharmacological testing of KCNH1 variants demonstrated differences in potency for channel block by imipramine and an investigational non-selective potassium channel blocker (LY97241) for specific variants. Collectively, our findings support gain-of-function as the most common, albeit not exclusive, molecular mechanism underlying KCNH1-related disorders and suggest opportunities for potential therapeutic drug repurposing strategies for these conditions. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Guarantors of Brain. FAU - Thompson, Christopher H AU - Thompson CH AUID- ORCID: 0000-0003-3713-630X AD - Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. FAU - Simmons, Christine Q AU - Simmons CQ AD - Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. FAU - He, Isabella K AU - He IK AD - Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. FAU - Jairam, Nirvani P AU - Jairam NP AD - Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. FAU - Desai, Reshma R AU - Desai RR AD - Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. FAU - Cha, Esther AU - Cha E AD - Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. FAU - Hong, Alexandra E AU - Hong AE AD - Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. FAU - DeKeyser, Jean-Marc AU - DeKeyser JM AD - Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. FAU - Laux, Linda C AU - Laux LC AD - Ann and Robert H. Lurie Children's Hospital, Chicago, IL 60611 USA. FAU - Jinnette, Michaelle AU - Jinnette M AD - Cure KCNH1 Foundation, Encinitas, CA 92024 USA. FAU - Vanoye, Carlos G AU - Vanoye CG AD - Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. FAU - George, Alfred L Jr AU - George AL Jr AUID- ORCID: 0000-0002-3993-966X AD - Department of Pharmacology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Brain JT - Brain : a journal of neurology JID - 0372537 SB - IM OTO - NOTNLM OT - electrophysiology OT - epilepsy OT - neurodevelopmental disorder OT - potassium channel EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 15:34 PHST- 2026/01/23 00:00 [received] PHST- 2026/08/30 00:00 [revised] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 15:34 [entrez] AID - 9003258 [pii] AID - 10.1093/brain/awag373 [doi] PST - aheadofprint SO - Brain. 2026 Oct 27:awag373. doi: 10.1093/brain/awag373. PMID- 42945471 OWN - NLM STAT- Publisher LR - 20261027 IS - 1613-6829 (Electronic) IS - 1613-6810 (Linking) DP - 2026 Oct 27 TI - Carbonyl Engineering in Covalent Organic Frameworks for Promoting Li(+) Conduction. LID - e76469 [pii] LID - 10.1002/smll.76469 [doi] AB - The rational design of covalent organic frameworks (COFs) for solid-state electrolytes (SSEs) is hindered by the precise position of polar functional groups (C horizontal line O) affect Li(+) transport. Herein, we synthesize four COFs materials with systematically carbonyl substitution positions to elucidate their structure-property relationships. Among them, the beta-ketoenamine-based COF featuring ortho-dicarbonyl groups exhibits a significantly enhanced electronegativity, which restricts anion migration and promotes selective Li(+) transport. Consequently, the rich-carbonly COF SSE achieves a high ionic conductivity of 9.83 x 10(-4) S/cm, a Li(+) transference number of 0.8, and an electrochemical stability window exceeding 5.1 V. Symmetric cell delivers stable Li plating/stripping for over 1200 h at 0.2 mA/cm(2) with a low overpotential of approximately 0.1 V, and over 460 h at 0.5 mA/cm(2). When coupled with a LiFePO(4) cathode, the solid-state battery retains 80% capacity after 1230 cycles at 1 C and 80.2% after 700 cycles at 5 C. Theoretical calculations reveal that the ortho-dicarbonyl coordination environment lowers the Li(+) migration barrier (0.376 eV) and enhances Li(+)-framework affinity, accelerating charge transfer. This work establishes a clear correlation from functional group positioning to pore chemistry, ion transport kinetics, and interfacial stability, offering a molecular-level design principle for high-performance COF-based solid-state electrolytes. CI - (c) 2026 Wiley-VCH GmbH. FAU - Zhang, Zhenhuan AU - Zhang Z AD - Institute of International Rivers and Eco-Security, Yunnan University, Kunming, China. FAU - Zhao, Genfu AU - Zhao G AUID- ORCID: 0009-0004-8591-2785 AD - International Joint Research Center for Advanced Energy Materials of Yunnan Province, Yunnan Key Laboratory of Carbon Neutrality and Green Low-Carbon Technologies, School of Materials and Energy, Yunnan University, Kunming, China. FAU - An, Qi AU - An Q AD - International Joint Research Center for Advanced Energy Materials of Yunnan Province, Yunnan Key Laboratory of Carbon Neutrality and Green Low-Carbon Technologies, School of Materials and Energy, Yunnan University, Kunming, China. FAU - Li, Wenwei AU - Li W AD - International Joint Research Center for Advanced Energy Materials of Yunnan Province, Yunnan Key Laboratory of Carbon Neutrality and Green Low-Carbon Technologies, School of Materials and Energy, Yunnan University, Kunming, China. FAU - Sun, Yongjiang AU - Sun Y AD - International Joint Research Center for Advanced Energy Materials of Yunnan Province, Yunnan Key Laboratory of Carbon Neutrality and Green Low-Carbon Technologies, School of Materials and Energy, Yunnan University, Kunming, China. FAU - Guo, Hong AU - Guo H AUID- ORCID: 0000-0001-5693-2980 AD - Institute of International Rivers and Eco-Security, Yunnan University, Kunming, China. AD - International Joint Research Center for Advanced Energy Materials of Yunnan Province, Yunnan Key Laboratory of Carbon Neutrality and Green Low-Carbon Technologies, School of Materials and Energy, Yunnan University, Kunming, China. FAU - Ma, Hang AU - Ma H AUID- ORCID: 0000-0002-4080-1609 AD - Research Institute, Yunnan Yuntianhua Co., Ltd, Kunming, China. LA - eng GR - 52303283/National Natural Science Foundation of China/ GR - 52372232/National Natural Science Foundation of China/ GR - 202403AA080019/Major Science and Technology Projects of Yunnan Province/ PT - Journal Article DEP - 20261027 PL - Germany TA - Small JT - Small (Weinheim an der Bergstrasse, Germany) JID - 101235338 SB - IM OTO - NOTNLM OT - carbonyl group OT - covalent organic framework materials OT - quasi-solid-state lithium metal batteries EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 15:33 PHST- 2026/10/16 00:00 [revised] PHST- 2026/09/04 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 15:33 [entrez] AID - e76469 [pii] AID - 10.1002/smll.76469 [doi] PST - aheadofprint SO - Small. 2026 Oct 27:e76469. doi: 10.1002/smll.76469. PMID- 42945465 OWN - NLM STAT- Publisher LR - 20261027 IS - 1613-6829 (Electronic) IS - 1613-6810 (Linking) DP - 2026 Oct 27 TI - Hollowing Out Nanofibers for Enhanced Visual Brightness and More Effective Solar Heating. LID - e76477 [pii] LID - 10.1002/smll.76477 [doi] AB - The temperature rise of a solar-heating textile is controlled by both generation and dissipation of heat. However, current designs mainly focus on increasing light absorption to generate more heat, often darkening the material while leaving thermal management largely overlooked. Herein, hollowing the nanofibers in a nonwoven mat produces a counterintuitive "brighter-yet-hotter" advantage over their solid (core-shell) counterparts. The polyacrylonitrile/polypyrrole double-shelled tubular nanofibers are fabricated by selectively removing the poly(epsilon-caprolactone) core from core-shell nanofibers. At outer and inner diameters of about 320 and 160 nm, the hollow nanofibers enhance backscattering in the visible range, making the mat brighter. Under 1 sun irradiation, the mat of hollow nanofibers shows a temperature rise of 28 degrees C, which is 12% greater than that of the paired solid counterpart. Thermal modeling indicates that the higher temperature rise originates from the internal channels, which disrupt heat propagation across the fibers and lower the rate of heat dissipation. Side-by-side comparisons under simulated and outdoor sunlight show that the higher-temperature region consistently appears on the mat of hollow nanofibers. This work highlights the promise of hollow nanofibers as a structural platform for optical and thermal management in solar-heating textiles. CI - (c) 2026 The Author(s). Small published by Wiley-VCH GmbH. FAU - Wu, Minghong AU - Wu M AUID- ORCID: 0000-0001-7918-695X AD - School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA. FAU - Meng, Yuxuan AU - Meng Y AUID- ORCID: 0009-0008-1373-1255 AD - School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA. FAU - Ji, Huajian AU - Ji H AD - School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA. FAU - He, Jianlong AU - He J AUID- ORCID: 0009-0008-2768-7689 AD - The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA. AD - Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, USA. FAU - Hao, Min AU - Hao M AUID- ORCID: 0009-0006-3517-3392 AD - The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA. AD - Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, USA. FAU - Xia, Younan AU - Xia Y AUID- ORCID: 0000-0003-2431-7048 AD - School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA. AD - School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia, USA. AD - The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA. AD - Department of Materials Science and Engineering, Johns Hopkins University, Baltimore, Maryland, USA. AD - Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland, USA. LA - eng GR - R01 NS126183/NS/NINDS NIH HHS/United States GR - Georgia Institute of Technology/ GR - Johns Hopkins University/ PT - Journal Article DEP - 20261027 PL - Germany TA - Small JT - Small (Weinheim an der Bergstrasse, Germany) JID - 101235338 SB - IM OTO - NOTNLM OT - heat dissipation OT - hollow nanofibers OT - light backscattering OT - solar heating OT - thermal management EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 15:23 PHST- 2026/10/15 00:00 [revised] PHST- 2026/08/25 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 15:23 [entrez] AID - e76477 [pii] AID - 10.1002/smll.76477 [doi] PST - aheadofprint SO - Small. 2026 Oct 27:e76477. doi: 10.1002/smll.76477. PMID- 42945456 OWN - NLM STAT- Publisher LR - 20261027 IS - 1526-0976 (Electronic) IS - 1089-2591 (Linking) DP - 2026 Oct 27 TI - Toward Safer Regenerative, Functional, and Aesthetic Gynecology: A Clinical Governance Framework for Patient Safety. LID - 10.1097/LGT.0000000000000994 [doi] AB - OBJECTIVES: Regenerative, functional, and aesthetic gynecology has expanded rapidly, yet discussions about complications remain focused on individual technologies rather than the clinical decisions that determine procedural safety. This review proposes a multidimensional clinical governance framework for complication prevention in this subspecialty. METHODS: A narrative review was based on a structured search of PubMed/MEDLINE and Embase (January 2014-May 2026), supplemented by international guidelines, regulatory communications, and evidence syntheses. Forty-two sources were included in the final qualitative synthesis. Evidence was appraised by study design, methodological rigor, clinical relevance, consistency, and applicability. The preliminary framework underwent successive rounds of multidisciplinary expert review, substantive textual and conceptual revision, and approval by all authors. RESULTS: Adverse outcomes arise from the convergence of 3 interdependent domains: patient-related factors (hormonal status, tissue quality, expectations, psychological context), clinician-related factors (diagnostic accuracy, anatomic expertise, endocrine competence, willingness to decline intervention), and procedural variables (technique, standardization, follow-up). When present, genitourinary syndrome of menopause represents a potentially modifiable biologic risk factor that should be identified and appropriately managed before restorative intervention is considered. A structured 5-component clinical evaluation framework integrating hormonal status, symptom profile, anatomic assessment, psychological context, and prior treatment history supports indication-based decision-making. Safety reflects both procedure-specific risk and patient-, clinician-, and protocol-related factors. CONCLUSIONS: Complication prevention begins with clinical reasoning, not procedural selection. The credibility of this subspecialty will depend on rigorous patient selection, endocrine optimization when indicated, evidence-based indications, competency-based training, and robust clinical governance rather than on the continuous introduction of new technologies. CI - Copyright (c) 2026, ASCCP. FAU - Sanchez-Borrego, Rafael AU - Sanchez-Borrego R AUID- ORCID: 0000-0001-6583-7440 AD - DIATROS Women's Clinic, Teknon Medical Center, Barcelona, Spain. FAU - Alinsod, Red AU - Alinsod R AUID- ORCID: 0000-0003-4859-0655 AD - Alinsod Institute for Aesthetic Vulvovaginal Surgery, Arlington, TX. FAU - Bader, Alexander AU - Bader A AUID- ORCID: 0000-0003-1914-113 AD - Bader Medical Institute, London, UK. FAU - Escribano, Juan Jose AU - Escribano JJ AUID- ORCID: 0009-0000-5913-5258 AD - Department of Obstetrics and Gynecology, Hospital Universitario Severo Ochoa, Madrid, Spain. FAU - Gaspar, Adrian AU - Gaspar A AUID- ORCID: 0000-0001-8852-9010 AD - Faculty of Medical Sciences, Second Chair of Gynecology, National University of Cordoba, Cordoba, Argentina. FAU - Gonzalez-Isaza, Pablo AU - Gonzalez-Isaza P AUID- ORCID: 0000-0001-7798-5141 AD - Department of Obstetrics and Gynecology, Hospital Universitario San Jorge, Soranus Center for Aesthetic and Regenerative Gynecology, Pereira, Colombia. AD - European College of Aesthetic Medicine and Surgery, Dublin, Ireland. FAU - Leibaschoff, Gustavo H AU - Leibaschoff GH AUID- ORCID: 0009-0005-3893-0849 AD - ICAM USA (International Consultants in Aesthetic Medicine), Dallas, TX. FAU - Sanchez-Prieto, Manuel AU - Sanchez-Prieto M AUID- ORCID: 0000-0001-7366-0446 AD - Menopause Unit, Dexeus University Institute, Barcelona, Spain. FAU - Goodman, Michael P AU - Goodman MP AUID- ORCID: 0000-0003-3880-7822 AD - The Labiaplasty and Vaginoplasty Training Institute of America, Davis, CA. AD - Department of Obstetrics and Gynecology, California Northstate University College of Medicine, Sacramento, CA. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Low Genit Tract Dis JT - Journal of lower genital tract disease JID - 9704963 SB - IM OTO - NOTNLM OT - clinical governance OT - complication prevention OT - female genital cosmetic surgery OT - genitourinary syndrome of menopause OT - patient safety OT - regenerative gynecology OT - vulvovaginal restorative procedures COIS- R.S.-B. reports consulting, speaking, and research relationships with Bayer, Exeltis, Mitra Pharmaceuticals, Perrigo, and Seid, unrelated to the present work; payments were made mainly to DIATROS SLP. R.A. reports consultancy and equity interests unrelated to the present work. A.G. reports educational collaborations with manufacturers of energy-based devices unrelated to the present work. P.G.-I. reports lecture and consultancy activities unrelated to the present work. G.H.L. reports scientific advisory activities through ICAM USA unrelated to the present work. The remaining authors have declared they have no conflicts of interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 15:12 PHST- 2026/07/14 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 15:12 [entrez] AID - 00128360-990000000-00279 [pii] AID - 10.1097/LGT.0000000000000994 [doi] PST - aheadofprint SO - J Low Genit Tract Dis. 2026 Oct 27. doi: 10.1097/LGT.0000000000000994. PMID- 42945455 OWN - NLM STAT- Publisher LR - 20261027 IS - 1944-8252 (Electronic) IS - 1944-8244 (Linking) DP - 2026 Oct 27 TI - Aggregation-Induced Photodegradable and Hydrolyzable Photosensitizer for Cu64 Positron Emission Tomography/Photoacoustic Imaging-Guided Phototherapy Nanoplatforms with Controllable Performance. LID - 10.1021/acsami.6c12177 [doi] AB - Radio imaging and optical imaging offer complementary advantages for biomedical diagnosis. However, the photosensitizers used in imaging must be degraded to reduce phototoxicity during phototherapy. Herein, we report a novel dicyanomethylene-based near-infrared photosensitizer (PS) that self-degrades via multiple pathways, including hydrolysis, aggregation-induced photodegradation, and oxidation by hypochlorous acid. To meet diverse theranostic requirements, the degradation behavior and performance of the PS were systematically regulated through co-assembly with three carriers. First, co-assembly with DSPE-PEG2000 produced a nanosystem that is stable to laser irradiation and water during multimodal diagnosis, and labeling with 64Cu enabled dual-mode photoacoustic and radio signal imaging. Second, co-assembly with PLGA-PEG5000 reduced the photodegradation rate by enabling complete degradation through hydrolysis, thereby facilitating deep-tumor phototherapy. Third, complexation with cucurbit[8]uril (CB[8]) selectively inhibited the hydrolytic properties of the photosensitizer, thereby reducing its phototoxicity through photodegradation and oxidative degradation pathways when superficial tumors are subjected to phototherapy. The phototherapeutic efficacy of the designed nanosystems was validated in a subcutaneous 4T1 tumor-bearing mouse model. The results demonstrated effective tumor inhibition with minimal phototoxicity, confirming the suitability of the developed system for safe phototherapy. Overall, this work presents a rational strategy for tuning photosensitizer degradation pathways to balance imaging performance, therapeutic efficacy, and biosafety, providing a versatile platform for multimodal theranostic applications. CI - (c) 2026 American Chemical Society. FAU - Chen, Hongyu AU - Chen H AD - School of Pharmacy, Thyroid and Breast Surgery, Affiliated Hospital of North Sichuan Medical College, North Sichuan Medical College, Nanchong637100, China. FAU - Feng, Jiao AU - Feng J AD - Sichuan Zhongjiu Guofeng Technology Co., Ltd., Mianyang621000, China. FAU - Ma, Yuan AU - Ma Y AD - School of Pharmacy, Thyroid and Breast Surgery, Affiliated Hospital of North Sichuan Medical College, North Sichuan Medical College, Nanchong637100, China. FAU - Zhu, Zechao AU - Zhu Z AD - School of Pharmacy, Thyroid and Breast Surgery, Affiliated Hospital of North Sichuan Medical College, North Sichuan Medical College, Nanchong637100, China. FAU - An, Feifei AU - An F AUID- ORCID: 0000-0001-7961-1709 AD - School of Public Health, Health Science Center, Xi'an Jiaotong University, No. 76 Yanta West Road, Xi'an, Shaanxi710061, China. FAU - Yuan, Xinye AU - Yuan X AD - College of Chemistry and Life Science, Sichuan Provincial Key Laboratory for Structural Optimization and Application of Functional Molecules, Chengdu Normal University, Chengdu611130, China. FAU - Wang, Jiexue AU - Wang J AD - College of Chemistry and Life Science, Sichuan Provincial Key Laboratory for Structural Optimization and Application of Functional Molecules, Chengdu Normal University, Chengdu611130, China. FAU - Deng, Guowei AU - Deng G AUID- ORCID: 0000-0001-7330-5874 AD - College of Chemistry and Life Science, Sichuan Provincial Key Laboratory for Structural Optimization and Application of Functional Molecules, Chengdu Normal University, Chengdu611130, China. FAU - Nie, Xufeng AU - Nie X AUID- ORCID: 0000-0003-0459-6692 AD - School of Pharmacy, Thyroid and Breast Surgery, Affiliated Hospital of North Sichuan Medical College, North Sichuan Medical College, Nanchong637100, China. FAU - Liu, Jun AU - Liu J AUID- ORCID: 0000-0002-4878-6466 AD - School of Pharmacy, Thyroid and Breast Surgery, Affiliated Hospital of North Sichuan Medical College, North Sichuan Medical College, Nanchong637100, China. AD - Medical Imaging Key Laboratory of Sichuan Province, North Sichuan Medical College, Nanchong, Sichuan, 637100, China. LA - eng GR - 21805019/National Natural Science Foundation of China/ GR - 81801768/National Natural Science Foundation of China/ GR - 2023JDRC0024/Natural Science Foundation of Sichuan Province/ GR - 23NSFSC1883/Natural Science Foundation of Sichuan Province/ PT - Journal Article DEP - 20261027 PL - United States TA - ACS Appl Mater Interfaces JT - ACS applied materials & interfaces JID - 101504991 SB - IM OTO - NOTNLM OT - aggregation-induced degradability OT - controllable self-degradation OT - hydrolysis OT - photoacoustic imaging OT - radio imaging EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 15:02 PHST- 2026/06/12 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 15:02 [entrez] AID - 5459372 [pii] AID - 10.1021/acsami.6c12177 [doi] PST - aheadofprint SO - ACS Appl Mater Interfaces. 2026 Oct 27. doi: 10.1021/acsami.6c12177. PMID- 42945436 OWN - NLM STAT- Publisher LR - 20261027 IS - 1662-8063 (Electronic) IS - 1662-4246 (Linking) DP - 2026 Oct 27 TI - Programmatic Considerations for Population-based Genomic Screening Implementation: An Expert-Informed Framework. PG - 1 LID - 10.1159/phg/aenag011 [doi] AB - PURPOSE: Population-based genomic screening (PGS) offers significant public health potential by identifying individuals at risk for clinically actionable genetic conditions. While existing frameworks guide gene selection based on clinical actionability, defining programmatic considerations, including ethical, economic, and equity-related factors, is necessary to support population-level implementation. This review aims to develop a set of programmatic considerations to guide PGS implementation decisions. METHODS: Using a structured, three-step deliberative process aligned with development of the Clinical Genome Resource (ClinGen) PGS Actionability Framework, multidisciplinary experts in public health genomics, clinical genetics, genetic counseling, ethics, health economics, and implementation science identified and refined key programmatic domains relevant to PGS implementation. RESULTS: Eight programmatic consideration domains were identified: (1) Program Evaluation, (2) Justice, (3) Feasibility, (4) Acceptability, (5) Respect for Autonomy, (6) Population-Level Costs, (7) Analytic Validity/Test Performance, and (8) Program Management. Each domain was defined and accompanied by guiding questions to support implementation planning, addressing critical gaps by incorporating population-level concerns such as equity, sustainability, and public trust. CONCLUSION: These programmatic considerations provide essential contextual guidance to support ethical and effective implementation of PGS programs. Used alongside gene-level actionability frameworks, they can inform how screening programs are designed, operationalized, and evaluated to better align genomic medicine with population health priorities. CI - (c) The Author(s) 2026. Published by S. Karger AG. FAU - Allen, Caitlin G AU - Allen CG FAU - Foss, Kimberly S AU - Foss KS FAU - Guan, Yue AU - Guan Y FAU - Manickam, Kandamurugu AU - Manickam K FAU - Smith, Hadley Stevens AU - Smith HS FAU - Buchanan, Adam H AU - Buchanan AH FAU - Hunter, Jessica Ezzell AU - Hunter JE FAU - Goldenberg, Aaron J AU - Goldenberg AJ LA - eng PT - Journal Article DEP - 20261027 PL - Switzerland TA - Public Health Genomics JT - Public health genomics JID - 101474167 SB - IM EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 14:52 PHST- 2026/04/16 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:52 [entrez] AID - 000555059 [pii] AID - 10.1159/phg/aenag011 [doi] PST - aheadofprint SO - Public Health Genomics. 2026 Oct 27:1. doi: 10.1159/phg/aenag011. PMID- 42945427 OWN - NLM STAT- Publisher LR - 20261027 IS - 1660-2862 (Electronic) IS - 1660-2854 (Linking) DP - 2026 Oct 27 TI - Structural Social Connection, Biological Ageing, and Neurodegenerative Disease Burden: A Global Ecological Analysis of Household Size and Modelled Dementia Incidence Estimates. PG - 1 LID - 10.1159/ndd/aduag008 [doi] AB - BACKGROUND: Poor social connection and ageing-related biological processes are recognised contributors to Alzheimer's disease and related dementias. However, cross-national evidence linking structural indicators of social connection with dementia and broader neurological dis-ease burden remains limited. This ecological study examined country-level associations be-tween average household size and modelled neurological disease incidence estimates. METHODS: Country- and territory-level data from up to 204 settings were analysed. Average household size was the primary exposure, and model-derived, age-standardised incidence-rate estimates for Alzheimer's disease and other dementias were the primary outcome. Par-kinson's disease, motor neuron disease, multiple sclerosis, idiopathic epilepsy, headache dis-orders, and total neurological disorders were examined as comparative secondary outcomes. The Henneberg Index, life expectancy at age 60, GDP per capita, and urban living were in-cluded as contextual covariates. Analyses included Pearson and Spearman correlations, par-tial correlations, principal component analysis, and multivariable linear regression. RESULTS: Household size was strongly and inversely associated with Alzheimer's disease and other dementias incidence estimates (Pearson r=-.841, p<.001). The association remained after adjustment for the included covariates (beta=-.569, p<.001). Significant inverse adjusted associations were also observed for Parkinson's disease, motor neuron disease, and total neurological disorders, whereas patterns differed for other neurological outcomes. Principal component analysis identified a single ageing-development dimension explaining 72.1% of the variance, with household size loading inversely against life expectancy at age 60, econom-ic affluence, urban living, and the Henneberg Index. CONCLUSION: Smaller average household size was associated with higher modelled incidence estimates for dementia and selected neurodegenerative diseases at the country level. These findings should be interpreted as ecological associations rather than evidence of individual-level causation and suggest that household structure is embedded within broader demograph-ic, socioeconomic, and ageing-related processes. CI - (c) The Author(s) 2026. Published by S. Karger AG. FAU - You, Wenpeng AU - You W LA - eng PT - Journal Article DEP - 20261027 PL - Switzerland TA - Neurodegener Dis JT - Neuro-degenerative diseases JID - 101189034 SB - IM EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 14:46 PHST- 2026/05/04 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:46 [entrez] AID - 000555057 [pii] AID - 10.1159/ndd/aduag008 [doi] PST - aheadofprint SO - Neurodegener Dis. 2026 Oct 27:1. doi: 10.1159/ndd/aduag008. PMID- 42945426 OWN - NLM STAT- Publisher LR - 20261027 IS - 1663-2826 (Electronic) IS - 1663-2818 (Linking) DP - 2026 Oct 27 TI - Limited Predictive Value of Genotype in GNAS-Related Disorders: Phenotypic Variability in a Cohort of 36 Pediatric Patients. PG - 1 LID - 10.1159/hrp/adaag049 [doi] AB - BACKGROUND: Pseudohypoparathyroidism (PHP) and related GNAS-associated disorders are rare conditions characterized by marked clinical and biochemical heterogeneity. Genotype-phenotype correlations remain complex due to genomic imprinting and parent-of-origin effects. OBJECTIVE: To evaluate genotype-phenotype correlations and intrafamilial variability in pediatric patients with GNAS-related disorders, with particular emphasis on familial cases. METHODS: A retrospective study was conducted in 36 patients (20 females, 16 males) from 22 unrelated families diagnosed with GNAS-related disorders. Genetic analysis included next-generation sequencing and, in selected cases, methylation studies. Variants were classified as confirmed loss-of-function (LOF), predicted LOF, or non-LOF according to ACMG criteria and in silico splice prediction. For genotype-phenotype analyses, predicted LOF variants were included in the LOF group. Clinical, biochemical, and anthropometric data were analyzed, and pedigree analysis was performed to assess inheritance patterns and intrafamilial variability. RESULTS: Genetic or epigenetic alterations involving the GNAS locus were identified in 26 of 36 patients (72.2%), including sequence variants in 24 patients and methylation defects in 2 patients. In 10 patients (27.8%), no molecular abnormality was identified, either due to lack of genetic testing or negative results. Among the 24 patients with sequence variants, 17 carried confirmed LOF variants according to ACMG classification. For genotype-phenotype analyses, two additional patients carrying the splice-region variant c.312+5G>A, predicted to impair splicing based on concordant predictions from multiple in silico tools, were included in the LOF group, resulting in a total of 19 LOF and 5 non-LOF patients. Patients with LOF variants demonstrated a significantly more severe biochemical phenotype, including higher PTH levels (p = 0.039), lower calcium levels (p = 0.018), higher phosphate levels (p = 0.013), and lower fT4 levels (p = 0.014). No significant differences were observed in TSH, BMI SDS, or height SDS. Pedigree analysis revealed marked intrafamilial variability, even among individuals carrying identical variants. Maternal inheritance predominated in familial cases. Notably, a clinically affected family without molecular confirmation was identified based on pedigree analysis. CONCLUSIONS: GNAS-related disorders demonstrate substantial phenotypic variability both between and within families. Loss-of-function variants are associated with a more severe biochemical phenotype, whereas clinical features are less discriminatory. Maternal inheritance predominates, consistent with imprinting mechanisms. Although loss-of-function variants were associated with a more severe biochemical phenotype, substantial inter- and intrafamilial variability limited the predictive value of genotype alone. These findings highlight the importance of integrated clinical, biochemical, and genetic evaluation, particularly in familial cases. CI - (c) The Author(s) 2026. Published by S. Karger AG. FAU - Roztoczynska, Dorota AU - Roztoczynska D FAU - Preizner-Rzucidlo, Ewelina AU - Preizner-Rzucidlo E FAU - Corica, Domenico AU - Corica D FAU - Janus, Dominika AU - Janus D FAU - Wedrychowicz, Anna AU - Wedrychowicz A FAU - Janeczko, Magdalena AU - Janeczko M FAU - Wasniewska, Malgorzata AU - Wasniewska M FAU - Starzyk, Jerzy B AU - Starzyk JB LA - eng PT - Journal Article DEP - 20261027 PL - Switzerland TA - Horm Res Paediatr JT - Hormone research in paediatrics JID - 101525157 SB - IM EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 14:45 PHST- 2026/05/06 00:00 [received] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:45 [entrez] AID - 000555055 [pii] AID - 10.1159/hrp/adaag049 [doi] PST - aheadofprint SO - Horm Res Paediatr. 2026 Oct 27:1. doi: 10.1159/hrp/adaag049. PMID- 42945423 OWN - NLM STAT- Publisher LR - 20261027 IS - 1423-0194 (Electronic) IS - 0028-3835 (Linking) DP - 2026 Oct 27 TI - Role of Substance P in physiological and pathological states of the pancreas. PG - 1 LID - 10.1159/nen/advag015 [doi] AB - Background Substance P (SP), a neuropeptide acting mainly through its high-affinity neurokinin-1 receptor (NK-1R), is a key signaling molecule in the pancreas. It is integral to the neuro-pancreatic axis, but its role shifts dramatically between physiological and pathological states. Summary Acting through NK-1R, SP plays various roles in the pancreatic physiological state and disease state. Under physiological conditions, SP contributes to the regulation of exocrine and endocrine functions. In pathological settings, SP signaling exerts effects on modulation of immunity, beta-cell preservation and insulin sensitivity in diabetes, triggers neurogenic inflammation and chronic pain in pancreatitis, and enhances malignant behaviours and perineural invasion in pancreatic cancer. Key Messages Functioning as an important regulator, SP maintains physiological balance in health yet drives disease progression in diabetes, pancreatitis, and pancreatic cancer. The SP/NK-1R axis has therapeutic potential with its beneficial actions. CI - (c) The Author(s) 2026. Published by S. Karger AG. FAU - Wang, Zhuoxin AU - Wang Z FAU - Liu, Yudan AU - Liu Y FAU - Sha, Lei AU - Sha L LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Switzerland TA - Neuroendocrinology JT - Neuroendocrinology JID - 0035665 SB - IM EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 14:45 PHST- 2025/12/16 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:45 [entrez] AID - 000555073 [pii] AID - 10.1159/nen/advag015 [doi] PST - aheadofprint SO - Neuroendocrinology. 2026 Oct 27:1. doi: 10.1159/nen/advag015. PMID- 42945419 OWN - NLM STAT- Publisher LR - 20261027 IS - 1421-9867 (Electronic) IS - 0012-2823 (Linking) DP - 2026 Oct 27 TI - Endoscopic Resection for Gastric Muscularis Propria-Derived Tumors: Clinical Outcomes from a Stepwise EFTR Implementation Program in 70 Patients. PG - 1 LID - 10.1159/dig/acmag032 [doi] AB - INTRODUCTION: This study aimed to evaluate the clinical outcomes of endoscopic resection for gastric muscularis propria-derived subepithelial lesions, including gastrointestinal stromal tumors, within a stepwise implementation program for endoscopic full-thickness resection (EFTR), and to identify factors associated with pathological R1 resection and technical difficulty. METHODS: This retrospective single-center study included consecutive patients who underwent endoscopic muscularis dissection (EMD), endoscopic resection with one-port placement (EROPP), or pure EFTR between January 2017 and January 2026. These techniques were introduced sequentially but selected according to lesion characteristics, technical feasibility, and institutional experience. Clinicopathological characteristics, procedural outcomes, pathological resection status, adverse events, and long-term outcomes were analyzed. Technical difficulty was assessed using a composite definition incorporating prolonged treatment time, major adverse events, pathological R1 resection, and the use of multiple over-the-scope clips. RESULTS: Seventy patients were included: 6 underwent EMD, 35 EROPP, and 29 pure EFTR. The median tumor size was 22 mm. Endoscopic complete resection was achieved in all patients, and pathological R0 resection in 62/70 (88.6%). Pathological R0 resection rates were 83.3%, 82.9%, and 96.5% for EMD, EROPP, and pure EFTR, respectively, with no significant difference among the three groups (P = 0.140). The median resection and closure times were 35 and 18 min, respectively. One major adverse event occurred: delayed perforation requiring endoscopic treatment. Larger tumor size was significantly associated with both pathological R1 resection and technical difficulty. During a median follow-up of 47 months for the entire cohort, no local recurrence or distant metastasis was observed. CONCLUSIONS: Endoscopic resection for selected gastric muscularis propria-derived tumors within a stepwise implementation program was associated with favorable long-term clinical outcomes. Tumor size was the principal factor associated with technical difficulty and pathological R1 resection. Pure EFTR provided favorable outcomes in appropriately selected lesions, while EROPP remained a complementary option for selected technically challenging lesions. These findings provide practical information for institutions considering the introduction of EFTR and support lesion-based procedural selection according to technical feasibility and institutional expertise. CI - (c) The Author(s) 2026. Published by S. Karger AG. FAU - Hirasawa, Kingo AU - Hirasawa K FAU - Sawada, Atsushi AU - Sawada A FAU - Sato, Chiko AU - Sato C FAU - Sato, Tsutomu AU - Sato T FAU - Sato, Sho AU - Sato S FAU - Azuma, Daisuke AU - Azuma D FAU - Nishio, Masafumi AU - Nishio M FAU - Kobayashi, Ryosuke AU - Kobayashi R FAU - Ikeda, Ryosuke AU - Ikeda R FAU - Atsusaka, Reo AU - Atsusaka R FAU - Morohashi, Keita AU - Morohashi K FAU - Kanemura, Tomoki AU - Kanemura T FAU - Nozaki, Kimio AU - Nozaki K FAU - Fujii, Satoshi AU - Fujii S FAU - Abe, Nobutsugu AU - Abe N FAU - Maeda, Shin AU - Maeda S LA - eng PT - Journal Article DEP - 20261027 PL - Switzerland TA - Digestion JT - Digestion JID - 0150472 SB - IM EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 14:44 PHST- 2026/07/06 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:44 [entrez] AID - 000555068 [pii] AID - 10.1159/dig/acmag032 [doi] PST - aheadofprint SO - Digestion. 2026 Oct 27:1. doi: 10.1159/dig/acmag032. PMID- 42945413 OWN - NLM STAT- Publisher LR - 20261027 IS - 1613-6829 (Electronic) IS - 1613-6810 (Linking) DP - 2026 Oct 27 TI - Modulating Ru-O Covalency Enables Suppressed Lattice Oxygen Pathway and Ru Dissolution for Robust Acidic Chlorine Evolution. LID - e76498 [pii] LID - 10.1002/smll.76498 [doi] AB - The chlorine evolution reaction (CER) is central to chlor-alkali electrolysis, yet Ru‑based anodes often suffer from competitive oxygen evolution and anodic dissolution under acidic potentials. Here, we report 2D ultrathin amorphous Sn‑doped RuO(x) nanosheets prepared via atomic engineering, in which Ru-O covalency and the Ru oxidation state are simultaneously modulated. Structural and spectroscopic analyses indicate that Ru centers are stabilized against over‑oxidation, suppressing the formation of volatile RuO(4) or soluble RuO(5) (2-). Operando attenuated total reflection surface-enhanced infrared absorption spectroscopy, combined with (18)O isotope labeling, reveals negligible lattice‑oxygen participation and a preference for direct Cl(-) adsorption, thereby suppressing oxygen evolution and promoting CER. The optimized Ru-based catalyst achieves high CER selectivity ( approximately 97%), long-term stability (>300 h), and mass activity nearly 40 times higher than commercial benchmarks. These results highlight the significant potential of metal-oxide bond-covalency modulation as a viable strategy for durable anodic oxidation electrocatalysis. CI - (c) 2026 Wiley-VCH GmbH. FAU - Ji, Jiapeng AU - Ji J AD - Shenzhen Key Laboratory of Energy Materials for Carbon Neutrality, Institute of Technology for Carbon Neutrality, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China. AD - Centre For Resource Innovation, Institute of Solid-State Physics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China. FAU - Mao, Zhixian AU - Mao Z AUID- ORCID: 0009-0002-5982-4445 AD - Centre For Resource Innovation, Institute of Solid-State Physics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China. AD - University of Science and Technology of China, Hefei, China. FAU - Chen, Shan AU - Chen S AUID- ORCID: 0000-0003-2138-4851 AD - Centre For Resource Innovation, Institute of Solid-State Physics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China. AD - University of Science and Technology of China, Hefei, China. FAU - Zhang, Xiaolong AU - Zhang X AUID- ORCID: 0000-0003-4695-5884 AD - Shenzhen Key Laboratory of Energy Materials for Carbon Neutrality, Institute of Technology for Carbon Neutrality, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, Guangdong, China. AD - Faculty of Materials Science and Energy Engineering, Shenzhen University of Advanced Technology, Shenzhen, Guangdong, China. FAU - Yin, Huajie AU - Yin H AUID- ORCID: 0000-0002-9036-9084 AD - Centre For Resource Innovation, Institute of Solid-State Physics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, China. AD - University of Science and Technology of China, Hefei, China. LA - eng GR - 52402136/National Natural Science Foundation of China/ GR - 2026A1515010180/Guangdong Natural Science Fund 2026-General Programme/ PT - Journal Article DEP - 20261027 PL - Germany TA - Small JT - Small (Weinheim an der Bergstrasse, Germany) JID - 101235338 SB - IM OTO - NOTNLM OT - 2D nanosheets OT - Ru horizontal line O bond modulation OT - anodic stability OT - chlorine evolution reaction EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 14:33 PHST- 2026/10/03 00:00 [revised] PHST- 2026/06/22 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:33 [entrez] AID - e76498 [pii] AID - 10.1002/smll.76498 [doi] PST - aheadofprint SO - Small. 2026 Oct 27:e76498. doi: 10.1002/smll.76498. PMID- 42945412 OWN - NLM STAT- Publisher LR - 20261027 IS - 1613-6829 (Electronic) IS - 1613-6810 (Linking) DP - 2026 Oct 27 TI - Polarity-Selective Engineering of In-Gap States in Amorphous Silicon Nitride via Distinct Structural and Electronic Origins. LID - e76480 [pii] LID - 10.1002/smll.76480 [doi] AB - Precise control of trap states in amorphous dielectrics requires identifying the fundamentally different physical origins of hole and electron trapping. Here, in amorphous silicon nitride (a‑SiN(x)), we demonstrate a dual-origin mechanism where the two trap polarities arise from decoupled structural and electronic mechanisms, enabling polarity-selective engineering of charge storage behavior. Multi-spectroscopic characterization reveals that hole traps are dictated by structural disorder, where Si-rich nanoclusters form a percolating network whose cluster-size statistics exhibit power-law scaling characteristic of disordered connectivity. In contrast, electron traps are governed by electronic-level alignment, where Hf incorporation lowers the Fermi level, activates deep in-gap states, and introduces additional shallow levels near the conduction-band edge-without perturbing the optimized nanocluster states. By linking structural topology and electronic alignment to polarity-resolved trapping, this work establishes a unified physical picture of trap polarity in disordered dielectrics and provides a practical design route for next-generation charge-trap memories and amorphous nitride/oxide electronic systems. CI - (c) 2026 Wiley-VCH GmbH. FAU - Ahn, Hanyeol AU - Ahn H AUID- ORCID: 0009-0007-6678-8258 AD - Department of Physics, University of Seoul, Seoul, South Korea. FAU - Gu, Minseon AU - Gu M AD - Department of Physics, University of Seoul, Seoul, South Korea. FAU - Kim, Hyun Don AU - Kim HD AUID- ORCID: 0009-0006-3217-0145 AD - Department of Physics, University of Seoul, Seoul, South Korea. AD - Department of Smart Cities, University of Seoul, Seoul, South Korea. FAU - Choi, Seungchul AU - Choi S AD - Department of Physics, University of Seoul, Seoul, South Korea. AD - Department of Smart Cities, University of Seoul, Seoul, South Korea. FAU - Park, Hyun Su AU - Park HS AUID- ORCID: 0009-0009-5072-8861 AD - Department of Physics, University of Seoul, Seoul, South Korea. FAU - Nam, Sangwoo AU - Nam S AUID- ORCID: 0009-0001-5163-8114 AD - Department of Physics, University of Seoul, Seoul, South Korea. AD - Department of Smart Cities, University of Seoul, Seoul, South Korea. FAU - Lee, Kyu-Myung AU - Lee KM AUID- ORCID: 0000-0001-9083-0275 AD - Department of Physics, Kyung Hee University, Seoul, South Korea. FAU - Kim, Moohyun AU - Kim M AD - Advanced Process Development Team, Semiconductor R&D Center, Samsung Electronics, Suwon, South Korea. FAU - Byun, Jinwoo AU - Byun J AD - Advanced Process Development Team, Semiconductor R&D Center, Samsung Electronics, Suwon, South Korea. FAU - Yon, Gukhyon AU - Yon G AD - Advanced Process Development Team, Semiconductor R&D Center, Samsung Electronics, Suwon, South Korea. FAU - Kim, Hyung Joon AU - Kim HJ AD - Advanced Process Development Team, Semiconductor R&D Center, Samsung Electronics, Suwon, South Korea. FAU - Park, Kwangmin AU - Park K AD - Advanced Process Development Team, Semiconductor R&D Center, Samsung Electronics, Suwon, South Korea. FAU - Choi, Hanmei AU - Choi H AD - Flash Process Development Team, Semiconductor R&D Center, Samsung Electronics, Suwon, South Korea. FAU - Kim, Jwasoon AU - Kim J AD - HB Solution, Daejeon, South Korea. FAU - Hahm, HaeJoon AU - Hahm H AD - HB Solution, Daejeon, South Korea. FAU - Kim, Soobang AU - Kim S AD - HB Solution, Daejeon, South Korea. FAU - Min, Won Ja AU - Min WJ AD - HB Solution, Daejeon, South Korea. FAU - Kwon, Da Mi AU - Kwon DM AD - National NanoFab Center (NNFC), Daejeon, South Korea. FAU - Park, Yun Chang AU - Park YC AUID- ORCID: 0000-0001-9840-4617 AD - National NanoFab Center (NNFC), Daejeon, South Korea. FAU - Kim, Gyungtae AU - Kim G AD - National NanoFab Center (NNFC), Daejeon, South Korea. FAU - Nguyen, Xuan Au AU - Nguyen XA AD - Department of Physics, Kyung Hee University, Seoul, South Korea. FAU - Kim, Tae Jung AU - Kim TJ AUID- ORCID: 0000-0001-8815-7560 AD - Department of Physics, Kyung Hee University, Seoul, South Korea. FAU - Kim, Young Dong AU - Kim YD AUID- ORCID: 0000-0002-4365-4544 AD - Department of Physics, Kyung Hee University, Seoul, South Korea. FAU - Park, Yongsup AU - Park Y AUID- ORCID: 0000-0002-8935-4221 AD - Department of Physics, Kyung Hee University, Seoul, South Korea. FAU - Choi, Eunjip AU - Choi E AD - Department of Physics, University of Seoul, Seoul, South Korea. FAU - Chang, Young Jun AU - Chang YJ AUID- ORCID: 0000-0001-5538-0643 AD - Department of Physics, University of Seoul, Seoul, South Korea. AD - Department of Intelligent Semiconductor, University of Seoul, Seoul, South Korea. FAU - Han, Moonsup AU - Han M AUID- ORCID: 0000-0003-0993-040X AD - Department of Physics, University of Seoul, Seoul, South Korea. LA - eng GR - IO251013-13737-01/Samsung Electronics Co., Ltd./ GR - 2018R1A6A1A06024977/NRF of Korea/ GR - 2020R1F1A1048651/NRF of Korea/ GR - RS-2023-00220471/NRF of Korea/ GR - RS-2024-00334854/NRF of Korea/ GR - PG2025039-02/Korea Basic Science Institute (National Research Facilities and Equipment Center)/ GR - NFEC-2012-03-160529/Korea Basic Science Institute (National Research Facilities and Equipment Center)/ PT - Journal Article DEP - 20261027 PL - Germany TA - Small JT - Small (Weinheim an der Bergstrasse, Germany) JID - 101235338 SB - IM OTO - NOTNLM OT - Hf doping OT - amorphous dielectric OT - charge trap OT - in-gap state OT - silicon nitride EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 14:33 PHST- 2026/10/06 00:00 [revised] PHST- 2026/05/18 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:33 [entrez] AID - e76480 [pii] AID - 10.1002/smll.76480 [doi] PST - aheadofprint SO - Small. 2026 Oct 27:e76480. doi: 10.1002/smll.76480. PMID- 42945410 OWN - NLM STAT- Publisher LR - 20261027 IS - 1613-6829 (Electronic) IS - 1613-6810 (Linking) DP - 2026 Oct 27 TI - Dual-Mode Separation of C(4) Hydrocarbons in Flexible Cu(ina)(2) MOF. LID - e76493 [pii] LID - 10.1002/smll.76493 [doi] AB - We present two synthetic routes to prepare thermodynamically stable copper-isonicotinate (Cu(ina)(2)) MOF, including direct in situ synthesis and thermal-induced irreversible phase transformation from metastable phase P. The P-->C transition proves that phase C is thermodynamically favorable. Both methods produce phase C with identical structure and show exceptional performance in C(4) hydrocarbon separation. At 298-308 K, phase C achieves an ultrahigh IAST selectivity of 15428.6 for n-butane/i-butane (n-C(4)H(10)/i-C(4)H(10)), and efficiently separates i-C(4)H(10) from butene isomers (n-butene/i-butene, n-C(4)H(8) / i-C(4)H(8)) and 1,3-butadiene (C(4)H(6)). The flexible framework also exhibits stimulus-responsive behavior toward i-C(4)H(8). Column breakthrough results demonstrate its excellent separation capability toward three typical C(4) mixtures: i-C(4)H(10)/i-C(4)H(8)/C(4)H(6), n-C(4)H(10)/i-C(4)H(10) and n-C(4)H(8)/i-C(4)H(8). As a flexible framework, Cu(ina)(2) realizes separation via a dual-mode mechanism of size exclusion and thermodynamic host-guest interactions. DFT and MD simulations reveal that pyridine ligand rotation triggers structural deformation and energy dissipation upon adsorption. The synergistic effect guarantees outstanding separation performance, and phase C is a promising benchmark adsorbent for C(4) hydrocarbon purification. CI - (c) 2026 Wiley-VCH GmbH. FAU - Zhao, Meng AU - Zhao M AUID- ORCID: 0000-0002-5163-1756 AD - Department of Applied Chemistry, College of Biotechnology and Food Science, Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin, People's Republic of China. FAU - Yang, Yun AU - Yang Y AD - Department of Applied Chemistry, College of Biotechnology and Food Science, Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin, People's Republic of China. FAU - Li, Tong AU - Li T AD - Department of Applied Chemistry, College of Biotechnology and Food Science, Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin, People's Republic of China. FAU - Sun, Lanlan AU - Sun L AUID- ORCID: 0009-0004-5690-2393 AD - Department of Applied Chemistry, College of Biotechnology and Food Science, Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin, People's Republic of China. FAU - Gu, Xue-Song AU - Gu XS AUID- ORCID: 0009-0007-8465-7593 AD - Department of Applied Chemistry, College of Biotechnology and Food Science, Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin, People's Republic of China. FAU - Shi, Yan AU - Shi Y AD - Department of Applied Chemistry, College of Biotechnology and Food Science, Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce, Tianjin, People's Republic of China. AD - School of Mechanical Engineering, Tianjin University of Commerce, Tianjin, People's Republic of China. LA - eng GR - 22308260/National Natural Science Foundation of China/ GR - 22402148/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261027 PL - Germany TA - Small JT - Small (Weinheim an der Bergstrasse, Germany) JID - 101235338 SB - IM OTO - NOTNLM OT - C4 separation OT - flexibility OT - metal-organic framework OT - phase transformation OT - size exclusion EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 14:33 PHST- 2026/09/29 00:00 [revised] PHST- 2026/07/30 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:33 [entrez] AID - e76493 [pii] AID - 10.1002/smll.76493 [doi] PST - aheadofprint SO - Small. 2026 Oct 27:e76493. doi: 10.1002/smll.76493. PMID- 42945407 OWN - NLM STAT- Publisher LR - 20261027 IS - 1613-6829 (Electronic) IS - 1613-6810 (Linking) DP - 2026 Oct 27 TI - Remote Plasma-Enabled Vertical Asymmetry Engineering of 2D PtSe(2) via Phase-Preserving Chalcogen Exchange. LID - e76465 [pii] LID - 10.1002/smll.76465 [doi] AB - Vertical asymmetry in two-dimensional (2D) transition metal dichalcogenides induces intrinsic dipoles, symmetry-breaking functionalities, and interface-specific electronic reconstruction. However, existing Janus synthesis processes rely on sequential treatments, high-temperature substitution, or solid-phase precursors. These approaches often cause phase transitions, lattice degradation, or defect formation, limiting scalability and device performance. Here, we introduce a remote plasma-enabled strategy for phase-preserving vertical asymmetry engineering in 2D Platinum Diselenide (PtSe(2)) via one-step chalcogen exchange. Using a single-reactor H(2)S remote plasma, treatment at near-room-temperature ( approximately 50 degrees C) selectively substitutes top-layer chalcogen atoms, forming Janus PtSSe with preserved crystallinity and minimal lattice perturbation. Increasing the temperature to 200-300 degrees C enables complete conversion to crystalline 2D Platinum Disulfide (PtS(2)). Janus PtSSe monolayers derived from exfoliated PtSe(2) exhibit an out-of-plane dipole and piezoresponse, with enhanced surface-enhanced Raman scattering relative to corresponding PtSe(2) and PtS(2) monolayers. Extending this approach from monolayers to four-layer (4L) PtSe(2) grown by metal-organic chemical vapor deposition, selective sulfurization produces Janus PtSSe/3L-PtSe(2) heterostructures with improved hydrogen evolution reaction kinetics relative to 4L-PtSe(2) and fully sulfurized 4L-PtS(2). Our results establish remote plasma-driven chalcogen exchange as a generalizable platform for symmetry-controlled 2D materials, enabling scalable engineering of vertical polarity, interfacial charge distribution, and functional responses. CI - (c) 2026 The Author(s). Small published by Wiley-VCH GmbH. FAU - Kim, Dong-Hyun AU - Kim DH AD - Institute of Advanced Composite Materials, Korea Institute of Science and Technology (KIST), Jeonbuk, Republic of Korea. AD - SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon, Republic of Korea. FAU - Moon, Byung Joon AU - Moon BJ AUID- ORCID: 0000-0002-6952-502X AD - Institute of Advanced Composite Materials, Korea Institute of Science and Technology (KIST), Jeonbuk, Republic of Korea. FAU - Gyeon, Minseung AU - Gyeon M AUID- ORCID: 0009-0007-5101-6351 AD - Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. AD - School of Electrical Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. FAU - Kim, Yuseok AU - Kim Y AD - Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. FAU - Kim, Jinwoo AU - Kim J AUID- ORCID: 0009-0005-7957-5556 AD - Department of Materials Science and Engineering, Seoul National University, Seoul, Republic of Korea. FAU - Sung, Dongchul AU - Sung D AD - Department of Physics, Graphene Research Institute, Quantum Information Science and Technology Center, KUU Quantum Materials.Devices International Research Center, and Quantum.AI Convergence Institute, Sejong University, Seoul, Republic of Korea. FAU - Park, Minji AU - Park M AD - Institute of Advanced Composite Materials, Korea Institute of Science and Technology (KIST), Jeonbuk, Republic of Korea. AD - Department of Materials Science and Engineering, Gwangju Institute of Science & Technology (GIST), Gwangju, Republic of Korea. FAU - Lee, Daekwon AU - Lee D AD - Department of Physics, Research Institute for Materials and Energy Sciences, Jeonbuk National University, Jeonju, Republic of Korea. FAU - Ra, Sooyeon AU - Ra S AD - Institute of Advanced Composite Materials, Korea Institute of Science and Technology (KIST), Jeonbuk, Republic of Korea. FAU - Lee, Han-Koo AU - Lee HK AUID- ORCID: 0009-0002-1711-9158 AD - Division of Advanced Nuclear Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea. AD - Pohang Accelerator Laboratory, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea. FAU - Im, Min Ji AU - Im MJ AD - Institute of Advanced Composite Materials, Korea Institute of Science and Technology (KIST), Jeonbuk, Republic of Korea. FAU - Jung, Unseok AU - Jung U AD - Institute of Advanced Composite Materials, Korea Institute of Science and Technology (KIST), Jeonbuk, Republic of Korea. FAU - Jeong, Yunjo AU - Jeong Y AUID- ORCID: 0000-0001-8236-8391 AD - Department of Mechanical Engineering, Texas A&M University, College Station, Texas, USA. FAU - Kim, Changheon AU - Kim C AD - Institute of Advanced Composite Materials, Korea Institute of Science and Technology (KIST), Jeonbuk, Republic of Korea. AD - Department of Materials Science and Engineering, Seoul National University, Seoul, Republic of Korea. FAU - Jang, Jimin AU - Jang J AD - Department of Materials Science and Engineering, Seoul National University, Seoul, Republic of Korea. FAU - Hyeong, Seok-Ki AU - Hyeong SK AD - Institute of Advanced Composite Materials, Korea Institute of Science and Technology (KIST), Jeonbuk, Republic of Korea. FAU - Kim, Taesung AU - Kim T AUID- ORCID: 0000-0001-6280-7668 AD - SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon, Republic of Korea. AD - School of Mechanical Engineering, Sungkyunkwan University, Suwon, Republic of Korea. FAU - Park, Sang Kyu AU - Park SK AUID- ORCID: 0000-0002-1184-7720 AD - Institute of Advanced Composite Materials, Korea Institute of Science and Technology (KIST), Jeonbuk, Republic of Korea. FAU - An, Sangmin AU - An S AUID- ORCID: 0000-0001-6534-2659 AD - Department of Physics, Research Institute for Materials and Energy Sciences, Jeonbuk National University, Jeonju, Republic of Korea. FAU - Yu, Jaehyung AU - Yu J AUID- ORCID: 0000-0002-9925-2309 AD - Department of Mechanical Engineering, Chung-Ang University, Seoul, Republic of Korea. FAU - Lee, Hunsu AU - Lee H AUID- ORCID: 0000-0001-9817-9835 AD - Institute of Advanced Composite Materials, Korea Institute of Science and Technology (KIST), Jeonbuk, Republic of Korea. FAU - Hong, Suklyun AU - Hong S AUID- ORCID: 0000-0001-6504-9209 AD - Department of Physics, Graphene Research Institute, Quantum Information Science and Technology Center, KUU Quantum Materials.Devices International Research Center, and Quantum.AI Convergence Institute, Sejong University, Seoul, Republic of Korea. FAU - Lee, Sanghan AU - Lee S AUID- ORCID: 0000-0002-5807-864X AD - Department of Materials Science and Engineering, Gwangju Institute of Science & Technology (GIST), Gwangju, Republic of Korea. FAU - Lee, Gwan-Hyoung AU - Lee GH AUID- ORCID: 0000-0002-3028-867X AD - Department of Materials Science and Engineering, Seoul National University, Seoul, Republic of Korea. AD - Institute of Engineering Research, Seoul National University, Seoul, Republic of Korea. AD - Institute of Applied Physics, Seoul National University, Seoul, Republic of Korea. FAU - Kang, Kibum AU - Kang K AUID- ORCID: 0000-0003-1674-1826 AD - Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. AD - Graduate School of Semiconductor Technology, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. FAU - Rho, Heesuk AU - Rho H AUID- ORCID: 0000-0003-2905-9180 AD - Department of Physics, Research Institute for Materials and Energy Sciences, Jeonbuk National University, Jeonju, Republic of Korea. FAU - Bae, Sukang AU - Bae S AUID- ORCID: 0000-0002-3019-0584 AD - Institute of Advanced Composite Materials, Korea Institute of Science and Technology (KIST), Jeonbuk, Republic of Korea. FAU - Lee, Changgu AU - Lee C AUID- ORCID: 0000-0002-8915-7746 AD - SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon, Republic of Korea. AD - School of Mechanical Engineering, Sungkyunkwan University, Suwon, Republic of Korea. FAU - Son, Jangyup AU - Son J AUID- ORCID: 0000-0002-1152-5422 AD - Institute of Advanced Composite Materials, Korea Institute of Science and Technology (KIST), Jeonbuk, Republic of Korea. AD - Division of Nano and Information Technology, KIST School University of Science and Technology (UST), Seoul, Republic of Korea. LA - eng GR - MSIT RS-2024-00452558/Nano & Material Technology Development Program through the National Research Foundation of Korea/ GR - 2022R1A3B1078163/Nano & Material Technology Development Program through the National Research Foundation of Korea/ GR - RS-2024-00433159/Nano & Material Technology Development Program through the National Research Foundation of Korea/ GR - RS-2024-00408845/Nano & Material Technology Development Program through the National Research Foundation of Korea/ GR - RS-2025-23323226/Nano & Material Technology Development Program through the National Research Foundation of Korea/ GR - RS-2024-00432340/Ministry of Trade, Industry & Energy of Korea/ GR - CRC20014-000/National Research Council of Science and Technology/ GR - RS-2025-23324133/Korea government MSIT/ GR - RS-2024-00355708/NRF/ GR - RS-2024-00436306/NRF/ GR - RS-2026-25609336/NRF/ GR - RS-2024-00437191/IITP/ GR - RS-2024-00443714/G-LAMP Program of NRF/ PT - Journal Article DEP - 20261027 PL - Germany TA - Small JT - Small (Weinheim an der Bergstrasse, Germany) JID - 101235338 SB - IM OTO - NOTNLM OT - Janus transition metal dichalcogenides OT - phase-preserving sulfurization OT - remote H2S plasma OT - surface atom substitution OT - vertical dipole engineering EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 14:23 PHST- 2026/10/02 00:00 [revised] PHST- 2026/05/13 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:23 [entrez] AID - e76465 [pii] AID - 10.1002/smll.76465 [doi] PST - aheadofprint SO - Small. 2026 Oct 27:e76465. doi: 10.1002/smll.76465. PMID- 42945406 OWN - NLM STAT- Publisher LR - 20261027 IS - 1613-6829 (Electronic) IS - 1613-6810 (Linking) DP - 2026 Oct 27 TI - Recent Advances and Challenges in Single-Atom Catalysts for Thermocatalytic CO(2) Hydrogenation. LID - e76194 [pii] LID - 10.1002/smll.76194 [doi] AB - Thermocatalytic hydrogenation of carbon dioxide (CO(2)) offers a promising route for carbon recycling and sustainable chemical production, yet its efficiency and selectivity are limited by the chemical inertness of CO(2) and the structural complexity of conventional heterogeneous catalysts. In recent years, single-atom catalysts (SACs) have emerged as an attractive platform for CO(2) hydrogenation due to their atomically defined active sites, high metal utilization efficiency, and tunable electronic structures. This review summarizes recent advances in SACs for thermocatalytic CO(2) hydrogenation, with emphasis on structure-activity relationships, reaction mechanisms, and selectivity control toward C(1) and economically more valuable C(2) (+) products. We highlight how coordination environment, metal-support interactions, and spatial confinement govern the electron transfer and catalytic performance. Emerging strategies such as cooperative and cascade catalysis, single atom-zeolite coupling, and confinement engineering are emphasized for overcoming intrinsic limitations of isolated single-atom sites. Finally, remaining challenges and future opportunities are outlined, focusing on catalyst stability, scalability, and the rational integration of SACs into industrially relevant catalytic architectures. CI - (c) 2026 Battelle Memorial Institute. Small published by Wiley-VCH GmbH. FAU - Chen, Ping AU - Chen P AD - Pacific Northwest National Laboratory, Richland, Washington, USA. AD - Zijin School of Geology and Mining, Fuzhou University, Fuzhou, Fujian, China. FAU - Zhang, Xin AU - Zhang X AUID- ORCID: 0000-0003-2000-858X AD - Pacific Northwest National Laboratory, Richland, Washington, USA. FAU - Shi, Honghong AU - Shi H AUID- ORCID: 0000-0002-9303-8344 AD - Pacific Northwest National Laboratory, Richland, Washington, USA. LA - eng GR - U.S. Department of Energy (DOE)/ GR - Office of Science/ GR - Office of Basic Energy Sciences (BES)/ GR - FWP# 56674/Chemical Sciences, Geosciences, and Biosciences Division (CSGB) through both the Geosciences Program/ GR - FWP#47319/and Catalysis Program (Advancing key catalytic reaction steps for achieving carbon neutrality)/ PT - Journal Article PT - Review DEP - 20261027 PL - Germany TA - Small JT - Small (Weinheim an der Bergstrasse, Germany) JID - 101235338 SB - IM OTO - NOTNLM OT - CO2 hydrogenation OT - heterogeneous catalysis OT - single-atom catalysts OT - thermocatalysis EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 14:23 PHST- 2026/06/29 00:00 [revised] PHST- 2026/01/17 00:00 [received] PHST- 2026/08/26 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:23 [entrez] AID - e76194 [pii] AID - 10.1002/smll.76194 [doi] PST - aheadofprint SO - Small. 2026 Oct 27:e76194. doi: 10.1002/smll.76194. PMID- 42945389 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 2767-3375 (Electronic) IS - 2767-3375 (Linking) VI - 6 IP - 10 DP - 2026 TI - Laboratory performance of antimicrobial susceptibility testing for GLASS pathogens among participants in the EQuAfrica programme, 2021‒2025. PG - e0007478 LID - 10.1371/journal.pgph.0007478 [doi] LID - e0007478 AB - The External Quality Assessment (EQA) for Africa (EQuAFRICA) project aimed at building regional capacity to provide a One Health bacteriology proficiency testing (PT) programme for reference and sentinel laboratories supporting antimicrobial resistance (AMR) surveillance and submission of data to the World Health Organization Global Antimicrobial Resistance and Use Surveillance System (WHO GLASS). We analysed the performance of participating laboratories in pathogen specific identification (ID) and qualitative antimicrobial susceptibility testing. The programme covered all GLASS and other bacterial pathogens of importance. One survey was distributed in 2021 and 2023 and two surveys in 2022, 2024 and 2025, with five isolates per survey. Participants were selected among reference and designated sentinel AMR laboratories from the 14 Fleming Fund recipient countries in Africa. The Clinical Microbiology Proficiency Testing (CMPT) marking scheme was used to score performance. The number of invited, enrolled and responding laboratories differed by survey; an average of 85% of enrolled laboratories submitted results. Participants scored 83% for organism ID across all cycles. Microbiology laboratories from the human health sector represented the majority in all cycles (75%). The average scores for antibiotic susceptibility testing (AST) was 73%, no participants that scored zero. Critical AST errors with clinical implications for directed treatment included vancomycin and cefoxitin for staphylococci. Our manuscript highlights that the use of EQA programmes to detecting gaps in laboratory processes and maintaining the quality and accuracy of AST is vital for effective AMR surveillance and control. This report illustrates that poorly performed AST at the beginning of the programme improved over the time. Building sustainable capacity for bacteriology testing, including AST, is critically important at all laboratory levels across Africa, from routine clinical and surveillance sites to national AMR reference laboratories. CI - Copyright: (c) 2026 Perovic et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Perovic, Olga AU - Perovic O AUID- ORCID: 0000-0001-5580-4629 AD - National Institute for Communicable Diseases (NICD) a division of National Health Laboratory Service, JohannesburgSouth Africa. AD - School of Pathology, University of Witwatersrand, Johannesburg, South Africa. FAU - van der Puije, Beatrice AU - van der Puije B AD - African Society for Laboratory Medicine (ASLM), Addis Ababa, Ethiopia. AD - African Field Epidemiology Network (AFENET), Kampala, Uganda. FAU - Carter, Jane Y AU - Carter JY AUID- ORCID: 0000-0003-4671-3519 AD - Amref Health Africa (Amref), Nairobi, Kenya. FAU - Humwa, Felix AU - Humwa F AD - African Society for Laboratory Medicine (ASLM), Addis Ababa, Ethiopia. FAU - Badat, Rubeina AU - Badat R AUID- ORCID: 0009-0002-5176-4118 AD - National Institute for Communicable Diseases (NICD) a division of National Health Laboratory Service, JohannesburgSouth Africa. FAU - Mwiti, Dennis AU - Mwiti D AUID- ORCID: 0009-0006-5295-6866 AD - Amref Health Africa (Amref), Nairobi, Kenya. FAU - Smith, Marshagne AU - Smith M AD - National Institute for Communicable Diseases (NICD) a division of National Health Laboratory Service, JohannesburgSouth Africa. FAU - Poulo Ly, Fatime AU - Poulo Ly F AUID- ORCID: 0009-0007-4641-2595 AD - Pole de Microbiologie, Institut Pasteur de Dakar, Dakar, Senegal. FAU - Fall, Cheikh AU - Fall C AD - Pole de Microbiologie, Institut Pasteur de Dakar, Dakar, Senegal. FAU - Dieye, Yakhya AU - Dieye Y AD - Pole de Microbiologie, Institut Pasteur de Dakar, Dakar, Senegal. FAU - Dabula, Patience AU - Dabula P AD - African Society for Laboratory Medicine (ASLM), Addis Ababa, Ethiopia. FAU - Hendriksen, Rene S AU - Hendriksen RS AUID- ORCID: 0000-0003-2934-8214 AD - National Food Institute (DTU Food), at Technical University of Denmark, Lyngby, Denmark. FAU - Tebeje, Yenew Kebede AU - Tebeje YK AD - Africa Centre for Diseases Control and Prevention (Africa CDC), Addis Ababa, Ethiopia. FAU - Ondoa, Pascale AU - Ondoa P AD - African Society for Laboratory Medicine (ASLM), Addis Ababa, Ethiopia. AD - The Global Fund to Fight AIDS, Tuberculosis and Malaria, Geneva, Switzerland. AD - Amsterdam Institute for Global Health and Development, Department of Global Health, University of Amsterdam, Amsterdam, the Netherlands. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLOS Glob Public Health JT - PLOS global public health JID - 9918283779606676 PMC - PMC13712707 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 14:13 PHST- 2026/04/07 00:00 [received] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:13 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PGPH-D-26-01241 [pii] AID - 10.1371/journal.pgph.0007478 [doi] PST - epublish SO - PLOS Glob Public Health. 2026 Oct 27;6(10):e0007478. doi: 10.1371/journal.pgph.0007478. eCollection 2026. PMID- 42945384 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Learner perceptions of AI-mediated affective support in L2 English learning: A cross-sectional survey. PG - e0360925 LID - 10.1371/journal.pone.0360925 [doi] LID - e0360925 AB - Emotional barriers can shape participation in second-language (L2) learning, yet evidence about affective support attributed to artificial intelligence (AI) remains conceptually and methodologically uneven. This cross-sectional online survey examined university-affiliated L2 English learners' reported emotional challenges, perceptions of AI-mediated affective support, and views of the boundary between AI and teacher support. The Chinese-language, researcher-developed questionnaire used categorical and multiple-response items rather than psychometric scales. Of 425 submissions, one non-university respondent and four respondents younger than 18 years were excluded, yielding an adult analytical sample of 420. The non-probability convenience sample was institutionally concentrated and should not be interpreted as representative of all Chinese university L2 learners. Frequencies, percentages, and three Pearson chi-square tests were calculated. Helplessness (59.05%), fatigue (55.95%), and anxiety (53.33%) were the most frequently reported emotional challenges. Overall, 67.38% perceived that AI could provide affective support, and 84.76% reported using AI for learning challenges. Immediate feedback was the most frequently selected support mechanism (79.52%), while relief of learning pressure or anxiety was the most frequently selected function (70.71%). Perceived AI affective support was associated with reported motivation change, chi(2)(2) = 53.27, p < .001, Cramer's V = .356, and interest change, chi(2)(2) = 59.57, p < .001, V = .377. The combined belief that AI might fill or replace teacher affective support was associated with continuing need for teacher support, chi(2)(1) = 72.97, p < .001, phi = .417. These results indicate patterned learner perceptions, not causal effects or evidence that AI possesses genuine emotion. AI may offer scalable, low-pressure support for routine learning, whereas teachers remain important for relational, contextual, and pedagogical judgment. Longitudinal studies using validated measures are needed. CI - Copyright: (c) 2026 Qiuxi Zhuo. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Zhuo, Qiuxi AU - Zhuo Q AUID- ORCID: 0000-0001-9462-2860 AD - School of Foreign Studies, Fuzhou University, Fuzhou, China. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Humans MH - *Artificial Intelligence MH - Cross-Sectional Studies MH - *Learning MH - Female MH - Male MH - Adult MH - Surveys and Questionnaires MH - *Multilingualism MH - *Perception MH - *Language MH - Emotions MH - Young Adult PMC - PMC13712676 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 14:13 PHST- 2026/01/14 00:00 [received] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:13 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PONE-D-26-00331 [pii] AID - 10.1371/journal.pone.0360925 [doi] PST - epublish SO - PLoS One. 2026 Oct 27;21(10):e0360925. doi: 10.1371/journal.pone.0360925. eCollection 2026. PMID- 42945379 OWN - NLM STAT- Publisher LR - 20261027 IS - 1553-7358 (Electronic) IS - 1553-734X (Linking) VI - 22 IP - 10 DP - 2026 Oct 27 TI - In silico prediction of metabolic trait robustness in microbial cells. PG - e1014864 LID - 10.1371/journal.pcbi.1014864 [doi] AB - Microbial strains undergo notable biomass expansion in industrial applications subjecting populations to Darwinian selection. The consequent adaptive evolution may threaten the often tediously developed desired metabolic traits of the strains, such as flavor or platform chemical production. Yet, it remains unresolved how to predict the evolutionary trait robustness. Here, we propose TRAEV (Trait Robustness Against EVolution), a computational framework for in silico prediction of evolutionary robustness of metabolic traits and trajectories of desired traits in application environments. TRAEV uses constraint-based metabolic models for predicting environment-dependent trait-fitness dependencies and accounts for the stochasticity of adaptive evolution by using Monte Carlo-based perturbation analysis. First, TRAEV predicts the immediate metabolic phenotype in the new environment, and then, the subsequent evolutionary trajectories of fitness and desired trait. Metabolic trait robustness is quantified from the evolutionary trajectories as two scores: Robustness Score (RS) and Trade-off Score (TS). RS represents trait robustness and the overall trait level during evolutionary adaptation, whereas TS captures trait-fitness trade-offs. We validated TRAEV by predicting relative evolutionary robustness of heterologous pigmentation of two engineered Saccharomyces cerevisiae strains and two desired aroma generation traits by multiple wine strains of S. cerevisiae if they were developed via a laboratory selection process. Thus, we showed how TRAEV predictions could guide strain development. TRAEV can be integrated into computational strain design workflows across microbial strains, desired metabolic traits, and application environments. Ultimately, model-predicted evolutionary robustness of desired metabolic traits can guide both strain and process development and help enhancing the economic attractiveness of industrial applications using microbial cells. CI - Copyright: (c) 2026 Gu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Gu, Chunhao AU - Gu C AUID- ORCID: 0009-0008-3556-7978 AD - Department of Bioproducts and Biosystems, Aalto University, Espoo, Finland. FAU - Mustonen, Ville AU - Mustonen V AD - Department of Computer Science, Department of Organismal and Evolutionary Biology, University of Helsinki, Helsinki, Finland. FAU - Jouhten, Paula AU - Jouhten P AUID- ORCID: 0000-0003-1075-7448 AD - Department of Bioproducts and Biosystems, Aalto University, Espoo, Finland. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLoS Comput Biol JT - PLoS computational biology JID - 101238922 SB - IM COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 14:13 PHST- 2025/12/31 00:00 [received] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:13 [entrez] AID - PCOMPBIOL-D-25-02748 [pii] AID - 10.1371/journal.pcbi.1014864 [doi] PST - aheadofprint SO - PLoS Comput Biol. 2026 Oct 27;22(10):e1014864. doi: 10.1371/journal.pcbi.1014864. PMID- 42945378 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2329-9185 (Electronic) IS - 2329-9185 (Linking) VI - 14 IP - 10 DP - 2026 Oct 1 TI - Advancing Value-Based Care in Orthopaedic Surgery: Lessons from Early Center for Medicare and Medicaid Innovation Models and a Framework for Sustainable Success. LID - e26.00141 [doi] AB - Adult reconstruction and adult spine surgeons practicing at Medicare-participating hospitals face expanding exposure to mandatory value-based payment models. The Transforming Episode Accountability Model began on January 1, 2026, in approximately 741 hospitals across 188 core-based statistical areas and includes lower-extremity joint replacement and spinal fusion among its 5 surgical episodes. The Expanded Comprehensive Care for Joint Replacement Model has been proposed for nationwide implementation by 2028. Pediatric orthopaedic practice and other subspecialties that primarily serve non-Medicare populations have limited direct exposure to these specific programs. Physician engagement in governance structures, including comanagement arrangements and clinically integrated networks, has been associated with more favorable clinical and financial outcomes under bundled payment programs. When evaluating value-based contracts, relevant considerations include the methodology for distributing reconciliation payments, risk-adjustment approach for both target prices and individual performance, and governance authority over protocol development and implant selection. The Centers for Medicare and Medicaid Services has finalized a mandatory patient-reported outcome measure for elective primary total hip and knee arthroplasty, with reporting beginning in the 2028 calendar year and tied to hospital payment determinations. The measure requires paired preoperative and 1-year postoperative outcome data from a majority of eligible patients. Comparable measures are anticipated for adult spine and other adult orthopaedic procedures over time. Pediatric orthopaedic practices are not subject to this measure in its current form. Early bundled payment programs achieved savings primarily through reductions in postacute care utilization, with complication, readmission, and mortality rates functioning as quality safeguards on that spending focus. A more complete framework for value measurement organizes metrics across a tiered hierarchy spanning care processes, short-term utilization and cost, intermediate clinical outcomes, and long-term patient-reported outcomes. The framework applies conceptually across orthopaedic subspecialties, including pediatrics, though current programs from the Center for Medicare and Medicaid Innovation incorporate its elements primarily in adult reconstruction and adult spine care. CI - Copyright (c) 2026 by The Journal of Bone and Joint Surgery, Incorporated. FAU - Balu, Abhinav R AU - Balu AR AUID- ORCID: 0000-0002-6242-7988 AD - Northwestern University Feinberg School of Medicine, Chicago, Illinois. FAU - Schwartz, Benjamin AU - Schwartz B AUID- ORCID: 0009-0005-7820-5756 AD - Commons Clinic, Long Beach, California. FAU - Pean, Christian AU - Pean C AUID- ORCID: 0000-0001-7445-1152 AD - Department of Orthopaedic Surgery, Duke University School of Medicine, Durham, North Carolina. FAU - Bozic, Kevin J AU - Bozic KJ AUID- ORCID: 0000-0001-9398-1239 AD - Department of Surgery and Perioperative Care, Dell Medical School, University of Texas at Austin, Austin, Texas. FAU - Louie, Philip K AU - Louie PK AUID- ORCID: 0000-0002-4787-1538 AD - Center for Neurosciences and Spine, Virginia Mason Franciscan Health, Seattle, Washington. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - United States TA - JBJS Rev JT - JBJS reviews JID - 101674872 SB - IM MH - United States MH - Humans MH - *Value-Based Health Care MH - *Orthopedic Procedures/economics MH - *Medicare MH - *Medicaid MH - Centers for Medicare and Medicaid Services, U.S. COIS- Disclosure: The Disclosure of Potential Conflicts of Interest forms are provided with the online version of the article (https://links.lww.com/JBJSREV/B407). EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 14:13 PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:13 [entrez] AID - 01874474-202610000-00008 [pii] AID - 10.2106/JBJS.RVW.26.00141 [doi] PST - epublish SO - JBJS Rev. 2026 Oct 27;14(10). doi: 10.2106/JBJS.RVW.26.00141. eCollection 2026 Oct 1. PMID- 42945376 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 2329-9185 (Electronic) IS - 2329-9185 (Linking) VI - 14 IP - 10 DP - 2026 Oct 1 TI - Total Hip and Knee Arthroplasty for Symptomatic Charcot Arthropathy. LID - e26.00091 [doi] AB - Charcot arthropathy of the hip and knee is a progressive neuropathy-driven joint destruction process that is frequently misdiagnosed and often presents with discordance between symptoms and radiographic severity, requiring a high index of suspicion. Initial management centers on nonoperative strategies-including off-loading, immobilization, and pharmacologic modulation of bone turnover-to halt disease progression rather than restore function. Total joint arthroplasty (total knee arthroplasty and total hip arthroplasty) can substantially improve pain and function in patients with symptomatic Charcot arthropathy who fail conservative treatment, despite limited high-level evidence. Arthroplasty in Charcot joints is technically demanding and associated with significantly higher complication rates (approximately 20%-30% for total knee arthroplasty and up to 45%-58% for total hip arthroplasty), with instability, infection, and mechanical failure among the most common complications. Successful outcomes depend on careful patient selection, optimization of modifiable risk factors (e.g., glycemic control), and appropriate implant selection (e.g., constrained or dual mobility constructs), underscoring the importance of meticulous preoperative planning and multidisciplinary care. CI - Copyright (c) 2026 by The Journal of Bone and Joint Surgery, Incorporated. FAU - Dean, Kristen K AU - Dean KK AUID- ORCID: 0009-0000-8441-8460 AD - Department of Orthopaedic Surgery, University of Miami Miller School of Medicine, Miami, Florida. FAU - Meli, Gabrielle M AU - Meli GM AUID- ORCID: 0009-0001-8532-8261 AD - Department of Orthopaedic Surgery, University of Miami Miller School of Medicine, Miami, Florida. FAU - Ference, Daisy A AU - Ference DA AUID- ORCID: 0009-0008-7453-4984 AD - Department of Orthopaedic Surgery, University of Miami Miller School of Medicine, Miami, Florida. FAU - Bendich, Ilya AU - Bendich I AUID- ORCID: 0000-0001-9917-9765 AD - Department of Orthopaedic Surgery, Washington University in St. Louis School of Medicine, St. Louis, Missouri. FAU - McNamara, Colin A AU - McNamara CA AUID- ORCID: 0000-0002-6013-9579 AD - Department of Orthopaedic Surgery, University of Miami Miller School of Medicine, Miami, Florida. FAU - Geiger, Erik J AU - Geiger EJ AUID- ORCID: 0000-0002-7487-6465 AD - Department of Orthopaedic Surgery, University of Miami Miller School of Medicine, Miami, Florida. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - United States TA - JBJS Rev JT - JBJS reviews JID - 101674872 SB - IM MH - Humans MH - *Arthropathy, Neurogenic/surgery MH - *Arthroplasty, Replacement, Knee/methods/adverse effects MH - *Arthroplasty, Replacement, Hip/methods/adverse effects COIS- Disclosure: The Disclosure of Potential Conflicts of Interest forms are provided with the online version of the article (https://links.lww.com/JBJSREV/B383). EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 14:13 PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:13 [entrez] AID - 01874474-202610000-00010 [pii] AID - 10.2106/JBJS.RVW.26.00091 [doi] PST - epublish SO - JBJS Rev. 2026 Oct 27;14(10). doi: 10.2106/JBJS.RVW.26.00091. eCollection 2026 Oct 1. PMID- 42945375 OWN - NLM STAT- MEDLINE DCOM- 20261028 LR - 20261029 IS - 2291-9694 (Electronic) IS - 2291-9694 (Linking) VI - 14 DP - 2026 Oct 27 TI - Beyond Time Saved: A Framework for Comprehensive Evaluation of AI Clinical Documentation. PG - e86091 LID - 10.2196/86091 [doi] LID - e86091 AB - Ambient AI scribes have been rapidly adopted across health care organizations to mitigate documentation burden and burnout. Initial single-system reports described substantial time savings, but more recent multisite evaluations and meta-analyses describe modest, heterogeneous effects with generally low methodological quality. Most evaluations emphasize efficiency and satisfaction, leaving potential effects on communication quality, narrative preservation, cognitive performance, and equity largely unmeasured. This viewpoint aimed to characterize evaluation patterns in prominent ambient AI scribe implementations, explain why unmeasured domains warrant investigation for patient safety and care quality, and propose an expanded framework with feasible research priorities. This analytic viewpoint mapped evaluation domain coverage in a purposive, illustrative sample of prominent implementation reports published through April 2026, then synthesized findings with literature on clinical communication, narrative medicine, automation effects, and health equity. Reporting was guided by Scale for the Assessment of Narrative Review Articles (SANRA) principles for narrative reviews; PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) was not used because the work is a domain-coverage characterization rather than an evidence synthesis estimating effects. A single reviewer (VSME) performed selection and extraction, consistent with the viewpoint's illustrative intent. Across the included implementations, evaluation consistently measured documentation time, after-hours work, and clinician satisfaction. Within this illustrative sample of 6 independently peer-reviewed real-world implementation evaluations, we did not identify any that reported systematic assessment of patient-clinician communication using validated instruments, structured comparison of AI-generated against human-generated notes for narrative fidelity, longitudinal evaluation of cognitive or educational effects, or equity analyses stratified by language and demographic group. The absence in this purposive sample reflects an absence of public reporting rather than proof that no organization has measured these dimensions internally. Limited error analyses found frequent omissions, with accuracy declining as encounter complexity increased. Evidence from multilingual and interpreter-mediated encounters was notably sparse. Current evidence supports continued implementation with quality safeguards in settings where benefits are demonstrated locally rather than universal adoption on the strength of early single-system reports. Organizations implementing ambient AI scribes should consider embedding prospective evaluation across 4 domains: validated communication quality measurement, structured narrative content comparison, longitudinal cognitive and training studies, and equity analyses across languages and demographics. Three near-term priorities offer immediate feasibility for organizations with evaluation capacity: (1) deploying validated communication instruments such as the Communication Assessment Tool or Consumer Assessment of Health Care Providers and Systems (CAHPS) within ongoing rollouts; (2) launching multisite longitudinal trainee cohorts comparing programs with and without ambient AI exposure; and (3) oversampling multilingual and interpreter-mediated encounters for stratified equity analyses. Narrative preservation requires foundational methodological work before implementation-ready measures exist. Editors, implementers, and vendors can each act now: editors by requiring domain-coverage reporting; implementers by preregistering evaluation plans before rollout; vendors by publishing stratified performance data as a routine condition of deployment. The goal is to preserve documented efficiency gains while generating evidence about communication, narrative, reasoning, and equity dimensions that current evaluations leave unmeasured. CI - (c) Ekstrom Victoria Sze Min. Originally published in JMIR Medical Informatics (https://medinform.jmir.org). FAU - Sze Min, Ekstrom Victoria AU - Sze Min EV AUID- ORCID: 0009-0004-9543-9688 AD - Department of Gastroenterology and Hepatology, Singapore General Hospital, 20 College Rd, Singapore, 169856, Singapore, 65 63214684. AD - Narratives in Medicine, SingHealth Duke NUS Medical Humanities Insititute, Singapore, Singapore. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Canada TA - JMIR Med Inform JT - JMIR medical informatics JID - 101645109 SB - IM MH - Humans MH - *Documentation/methods/standards MH - *Artificial Intelligence MH - Narration MH - *Quality of Health Care/organization & administration/standards MH - Efficiency, Organizational MH - Communication MH - Clinical Reasoning MH - Health Equity/organization & administration/standards PMC - PMC13712405 OTO - NOTNLM OT - ambient scribes OT - automation bias OT - clinical documentation OT - clinical reasoning OT - health equity OT - implementation science OT - narrative medicine OT - patient-clinician communication COIS- Conflicts of Interest: The author has used AI documentation tools developed by SingHealth in clinical practice since 2024. Experience has been mixed, with clear efficiency benefits for straightforward encounters in English, but observed challenges in multilingual consultations involving Mandarin and Cantonese code-switching, family-mediated decision-making with multiple speakers, and complex cases involving substantial emotional or psychosocial context requiring nuanced narrative preservation. Specific clinical encounters where AI systems struggled with speaker attribution in family consultations involving elderly patients, adult children, and the author simultaneously, and instances where AI-generated summaries omitted emotional context about patient fears regarding familial cancer patterns that were relevant to shared decision-making about screening intensity, motivated attention toward equity and narrative preservation concerns in this analysis. These observations are subjective clinical impressions from a tertiary gastroenterology practice serving diverse immigrant populations and may not represent typical patterns across other clinical settings. The author's institution has not conducted a formal internal evaluation informing this work beyond routine clinical quality review processes. No financial relationships with AI vendors or parent companies exist. This viewpoint reflects the author's personal analysis and does not represent the institutional positions of Singapore General Hospital or SingHealth Duke-NUS Medical Humanities Institute. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 14:13 PHST- 2025/10/18 00:00 [received] PHST- 2026/08/25 00:00 [revised] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:13 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - v14i1e86091 [pii] AID - 86091 [pii] AID - 10.2196/86091 [doi] PST - epublish SO - JMIR Med Inform. 2026 Oct 27;14:e86091. doi: 10.2196/86091. PMID- 42945373 OWN - NLM STAT- Publisher LR - 20261027 IS - 1553-7404 (Electronic) IS - 1553-7390 (Linking) VI - 22 IP - 10 DP - 2026 Oct 27 TI - CDH1 loss remodels gene expression and lineage identity in human mammary epithelial cells. PG - e1012351 LID - 10.1371/journal.pgen.1012351 [doi] AB - Invasive lobular carcinoma (ILC) is a common subtype of breast cancer, molecularly defined by genetic loss of CDH1, and subsequent loss of cell adhesion protein E-cadherin, in ~95% of ILC. Though CDH1 loss occurs early in ILC oncogenesis, it is unclear how this facilitates transformation. We modeled early CDH1 loss using "normal" human mammary epithelial cells (HMEC), i.e., finite lifespan cells reflecting early hyperplasia, and targeted E-cadherin signaling using antibodies versus causing genetic CDH1 loss using siRNA or CRISPR/Cas9-knockout. Transcriptome analysis across four HMEC models showed that the mode of E-cadherin targeting is critical for the subsequent phenotype. Antibody-mediated inhibition of cell-cell contacts induced gene signatures of epithelial-mesenchymal transition (EMT), consistent with the role of E-cadherin suppression during the EMT process. Conversely, genetic CDH1 loss - as in ILC oncogenesis - repressed EMT signatures, and instead remodeled gene expression toward a luminal epithelial phenotype. RNA-seq, single cell transcriptomics, flow cytometry, microscopy, and ATAC-seq analyses support that CDH1 loss induces lineage remodeling to a luminal state, which is mirrored in transcriptomic analysis of clinical ILC precursor lesions. By isolating luminal versus basal cells prior to CDH1 knockout, we found that CDH1 loss led to remodeling of lineage identity in both populations, converging on a new lineage homeostasis with a luminal progenitor-like phenotype. Consistent with the shift to a luminal progenitor phenotype, CDH1 loss enhanced proliferative capacity over the finite lifespan of the HMECs, highlighting a feature of early CDH1 loss that may contribute to clonal advantage during tumor initiation. Moreover, CDH1 loss enhanced anoikis resistance, a defining feature of ILC cells. Our findings support that genetic loss of CDH1 in mammary epithelial cells induces transcriptional and phenotypic changes consistent with lineage identity remodeling toward a luminal progenitor-like state, which may underpin the mechanism by which early CDH1 loss mediates ILC oncogenesis. CI - Copyright: (c) 2026 Musick et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Musick, Maggie AU - Musick M AUID- ORCID: 0000-0002-3564-2527 AD - Department of Pathology, University of Colorado Anschutz, Aurora, Colorado, United States of America. FAU - Ufondu, Chinasa A AU - Ufondu CA AUID- ORCID: 0009-0007-3883-2936 AD - Department of Pharmacology, Molecular Pharmacology and Therapeutics (MPaT) Program, University of Minnesota, Twin Cities, Minneapolis, Minnesota, United States of America. FAU - Rowland, Carmen E AU - Rowland CE AD - Department of Pathology, University of Colorado Anschutz, Aurora, Colorado, United States of America. FAU - Brown, Micaela E AU - Brown ME AD - Department of Pathology, University of Colorado Anschutz, Aurora, Colorado, United States of America. FAU - Sottnik, Joseph L AU - Sottnik JL AUID- ORCID: 0000-0002-0263-3608 AD - Department of Pathology, University of Colorado Anschutz, Aurora, Colorado, United States of America. FAU - Shackleford, Madeleine T AU - Shackleford MT AD - Department of Pathology, University of Colorado Anschutz, Aurora, Colorado, United States of America. FAU - Nesiba, Camryn S AU - Nesiba CS AD - Department of Pathology, University of Colorado Anschutz, Aurora, Colorado, United States of America. FAU - Danis, Etienne P AU - Danis EP AUID- ORCID: 0000-0002-5651-8111 AD - University of Colorado Cancer Center, University of Colorado Anschutz, Aurora, Colorado, United States of America. AD - Department of Biomedical Informatics, University of Colorado Anschutz, Aurora, Colorado, United States of America. FAU - Goodspeed, Andrew E AU - Goodspeed AE AD - University of Colorado Cancer Center, University of Colorado Anschutz, Aurora, Colorado, United States of America. AD - Department of Biomedical Informatics, University of Colorado Anschutz, Aurora, Colorado, United States of America. FAU - Zera, Richard AU - Zera R AD - Hennepin County Medical Center, Minneapolis, Minnesota, United States of America. FAU - Johnson, Arthur T AU - Johnson AT AD - Hennepin County Medical Center, Minneapolis, Minnesota, United States of America. FAU - Schwertfeger, Kathryn L AU - Schwertfeger KL AD - Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, United States of America. AD - Masonic Cancer Center, University of Minnesota, Twin Cities, Minneapolis, Minnesota, United States of America. FAU - Nelson, Andrew C AU - Nelson AC AUID- ORCID: 0000-0002-4741-0010 AD - Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, Minnesota, United States of America. FAU - Ostrander, Julie H AU - Ostrander JH AUID- ORCID: 0000-0002-5038-6875 AD - Masonic Cancer Center, University of Minnesota, Twin Cities, Minneapolis, Minnesota, United States of America. FAU - Sikora, Matthew J AU - Sikora MJ AUID- ORCID: 0000-0003-2915-7442 AD - Department of Pathology, University of Colorado Anschutz, Aurora, Colorado, United States of America. AD - University of Colorado Cancer Center, University of Colorado Anschutz, Aurora, Colorado, United States of America. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLoS Genet JT - PLoS genetics JID - 101239074 SB - IM COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 14:13 PHST- 2026/06/03 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:13 [entrez] AID - PGENETICS-D-26-00592 [pii] AID - 10.1371/journal.pgen.1012351 [doi] PST - aheadofprint SO - PLoS Genet. 2026 Oct 27;22(10):e1012351. doi: 10.1371/journal.pgen.1012351. PMID- 42945372 OWN - NLM STAT- Publisher LR - 20261027 IS - 1553-7358 (Electronic) IS - 1553-734X (Linking) VI - 22 IP - 10 DP - 2026 Oct 27 TI - KmerSignificance score: A discriminative and biologically informed framework for viral k-mer prioritization. PG - e1014332 LID - 10.1371/journal.pcbi.1014332 [doi] AB - Distinguishing closely related viral strains requires identifying genomic regions where subtle sequence differences carry biological significance. While k-mer-based approaches offer computational efficiency for genome analysis, existing methods lack standardized frameworks for evaluating which k-mers are most informative. Current selection strategies focus primarily on statistical discriminative power without integrating biological context. We introduce KmerSignificance Score (KSS), a k-mer prioritization framework combining three components: an information-theoretic method measuring strain-distinguishing capacity, an optimized amino acid substitution matrix (MIYATA_EVO) for mutation impact assessment, and a protein characterization score derived from annotations and database cross-references retrieved through UniProt. All three component scores and the integrated KSS are bounded in [0,1] by construction, providing a common numerical scale for ranking positions within a dataset. The discriminative component achieved a mean F1 of 0.881, not significantly different from chi-squared (0.876), the strongest of eight baselines. Top-ranked selections from the discriminative component nonetheless differed from those produced by chi-squared, especially for SARS-CoV-2 and HIV-1. The MIYATA_EVO matrix, optimized using a genetic algorithm, increased the composite agreement score across eight amino acid characteristics by 28.4% over the original MIYATA matrix. Across 17,471 viral proteins, the protein characterization score tracked UniProt's five-level annotation score closely (Kendall's tau=0.775), as expected for measures drawing on overlapping annotation content. Within each level, the continuous score provided finer numerical resolution among proteins. Literature cross-referencing on SARS-CoV-2 (278,738 sequences, 19 Pango-derived lineage labels), HIV-1 (12,223 sequences, 15 subtype labels), and human cytomegalovirus (HCMV; 399-646 sequences, 4-8 locus-specific genotypes) found that the top-ranked k-mers reported here map to established variant-defining mutations, subtype-specific polymorphisms, and genotype markers. KSS provides a standardized framework for viral k-mer prioritization with potential applications in variant surveillance, molecular epidemiology, and functional annotation. The tool is available at https://github.com/bioinfoUQAM/KmerSignificanceScore. CI - Copyright: (c) 2026 Lebatteux et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Lebatteux, Dylan AU - Lebatteux D AUID- ORCID: 0009-0001-8896-8838 AD - Department of Computer Science, Universite du Quebec a Montreal, Montreal, Quebec, Canada. AD - Centre de recherche Azrieli du CHU Sainte-Justine, Montreal, Quebec, Canada. AD - Cite des Sciences et de l'Innovation de Guinee, Conakry, Guinea. FAU - Corso, Florian AU - Corso F AD - Centre de recherche Azrieli du CHU Sainte-Justine, Montreal, Quebec, Canada. AD - Department of Microbiology, Infectiology and Immunology, Faculty of Medicine, Universite de Montreal, Montreal, Quebec, Canada. FAU - Soudeyns, Hugo AU - Soudeyns H AUID- ORCID: 0000-0001-5857-3176 AD - Centre de recherche Azrieli du CHU Sainte-Justine, Montreal, Quebec, Canada. AD - Department of Microbiology, Infectiology and Immunology, Faculty of Medicine, Universite de Montreal, Montreal, Quebec, Canada. AD - Department of Pediatrics, Faculty of Medicine, Universite de Montreal, Montreal, Quebec, Canada. AD - Pathology Advanced Translational Research Unit (PATRU), Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, United States of America. FAU - Boucoiran, Isabelle AU - Boucoiran I AD - Centre de recherche Azrieli du CHU Sainte-Justine, Montreal, Quebec, Canada. AD - Department of Obstetrics and Gynecology, Faculty of Medicine, Universite de Montreal, Montreal, Quebec, Canada. FAU - Gantt, Soren AU - Gantt S AD - Centre de recherche Azrieli du CHU Sainte-Justine, Montreal, Quebec, Canada. AD - Department of Microbiology, Infectiology and Immunology, Faculty of Medicine, Universite de Montreal, Montreal, Quebec, Canada. AD - Department of Pediatrics, Faculty of Medicine, Universite de Montreal, Montreal, Quebec, Canada. FAU - Banire Diallo, Abdoulaye AU - Banire Diallo A AD - Department of Computer Science, Universite du Quebec a Montreal, Montreal, Quebec, Canada. AD - Cite des Sciences et de l'Innovation de Guinee, Conakry, Guinea. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLoS Comput Biol JT - PLoS computational biology JID - 101238922 SB - IM COIS- SG reports research funding and consulting fees from Moderna, Merck, Pfizer, GSK and Curevo Vaccines. IB is funded by a Fond de Recherche du Quebec salary award. IB has received consultant fees from Moderna and Pfizer, and research support from Altona and Moderna outside of the submitted work. The other authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 14:13 PHST- 2026/05/12 00:00 [received] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:13 [entrez] AID - PCOMPBIOL-D-26-01137 [pii] AID - 10.1371/journal.pcbi.1014332 [doi] PST - aheadofprint SO - PLoS Comput Biol. 2026 Oct 27;22(10):e1014332. doi: 10.1371/journal.pcbi.1014332. PMID- 42945362 OWN - NLM STAT- Publisher LR - 20261027 IS - 1554-8937 (Electronic) IS - 1554-8929 (Linking) DP - 2026 Oct 27 TI - Pharmacological ON/OFF Regulation of CAR T Cells through Estrogen Receptor-Based Stabilization and Targeted Degradation. LID - 10.1021/acschembio.6c00722 [doi] AB - Regulation of therapeutic cell response is important for safe and effective therapy, particularly for immunotherapy. Ideally, the regulators should be based on human proteins using compounds that have already been approved for human use. Regulation of protein degradation by small molecules enables fast cellular response and a small genetic footprint of genetic constructs. Here, we present a clinically compatible strategy for reversible pharmacological control of chimeric antigen receptor (CAR) T cell function using human estrogen receptor (ER)-based degron domains and FDA-approved small molecules. By fusing tamoxifen-responsive ER ligand-binding domains to CARs, we generate ligand-inducible ON-switch CARs whose stability, signaling, and effector functions are precisely controlled by 4-hydroxytamoxifen. Furthermore, we demonstrate that ER-tagged CARs can be selectively degraded using the FDA-approved ER-targeting PROTAC ARV-471, establishing a complementary OFF-switch mechanism that suppresses CAR expression and effector function. Notably, these results expand the ON-OFF switch platform for post-translational control of therapeutic proteins, offering new opportunities to improve the safety and precision of cellular immunotherapies. CI - (c) 2026 The Author(s). Published by American Chemical Society. FAU - Rihtar, Erik AU - Rihtar E AD - Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, Ljubljana1000, Slovenia. FAU - Fink, Tina AU - Fink T AD - Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, Ljubljana1000, Slovenia. AD - Centre for Technologies of Gene and Cell Therapy, Hajdrihova 19, Ljubljana1000, Slovenia. FAU - Belak, Monika AU - Belak M AD - Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, Ljubljana1000, Slovenia. FAU - Udvanc, Rebeka AU - Udvanc R AD - Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, Ljubljana1000, Slovenia. FAU - Jerala, Roman AU - Jerala R AUID- ORCID: 0000-0002-6337-5251 AD - Department of Synthetic Biology and Immunology, National Institute of Chemistry, Hajdrihova 19, Ljubljana1000, Slovenia. AD - Centre for Technologies of Gene and Cell Therapy, Hajdrihova 19, Ljubljana1000, Slovenia. LA - eng GR - 101059842/Horizon 2020 Framework Programme/ GR - J3-60061/The Slovenian Research and Innovation Agency/ GR - J7-4493/The Slovenian Research and Innovation Agency/ GR - J7-4640/The Slovenian Research and Innovation Agency/ GR - P4-0176/The Slovenian Research and Innovation Agency/ PT - Journal Article DEP - 20261027 PL - United States TA - ACS Chem Biol JT - ACS chemical biology JID - 101282906 SB - IM EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 14:04 PHST- 2026/08/10 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:04 [entrez] AID - 5459340 [pii] AID - 10.1021/acschembio.6c00722 [doi] PST - aheadofprint SO - ACS Chem Biol. 2026 Oct 27. doi: 10.1021/acschembio.6c00722. PMID- 42945356 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1935-2735 (Electronic) IS - 1935-2727 (Print) IS - 1935-2727 (Linking) VI - 20 IP - 10 DP - 2026 Oct TI - Occupational brucellosis among livestock farmers in Northeastern Thailand: A cross-sectional study of knowledge, attitudes, and practices from a One Health perspective. PG - e0014788 LID - 10.1371/journal.pntd.0014788 [doi] LID - e0014788 AB - Brucellosis remains a significant occupational zoonotic disease among livestock farmers, with substantial implications for animal health, worker safety, and public health in endemic regions. Effective control at the livestock-human interface depends on understanding farmers' knowledge, attitudes, and practices (KAP) regarding disease prevention. This study aimed to assess determinants of occupational brucellosis prevention behaviors among ruminant farmers in Northeastern Thailand through comprehensive KAP analysis to inform targeted One Health strategies. A cross-sectional survey was conducted from October 2024 to July 2025 across three provinces (Nakhon Ratchasima, Buriram, and Surin) in lower Northeastern Thailand. Structured questionnaires were administered to 385 livestock farmers managing ruminants (beef cattle, dairy cattle, buffalo, goats, or sheep). Knowledge, attitude, and practice domains were scored and analyzed using logistic regression to identify behavioral determinants. Although 65.5% of farmers demonstrated satisfactory knowledge, only 20.0% reported comprehensive favorable preventive practices across personal and farm-level measures. Notably, farmers exhibited selective engagement with prevention: while all avoided raw milk and placental product consumption, only 38.7% used gloves during high-risk activities compared to 61.6% wearing boots. Multivariate analysis identified farm certification, including Good Farm Management as shown by adjusted Odds Ratio (aOR) 2.60 (95% CI: 1.06-6.39), and brucellosis-free certification as shown by aOR 5.35 (95% CI: 1.63-17.54). Awareness of having heard regarding the scientific term "brucellosis" was associated with favorable practices (aOR 2.74, 95% CI: 1.00-7.46). In conclusion, knowledge alone was insufficient to drive consistent preventive behaviors, instead, farm certification, targeted education on transmission routes, and perceived occupational susceptibility emerged as key drivers of practice adoption. Our findings highlight the necessity for integrated interventions combining domain-specific training, formal disease alerts, and structural supports such as certification programs to enhance occupational safety and brucellosis control aligned with One Health principles in Thailand. CI - Copyright: (c) 2026 Kiatsomphob et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Kiatsomphob, Savek AU - Kiatsomphob S AD - Department of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand. FAU - Suphaphiphat, Karunasinee AU - Suphaphiphat K AD - Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand. FAU - Puyati, Bopit AU - Puyati B AD - Buriram Provincial Livestock Office, Buriram, Thailand. FAU - Kledmanee, Kan AU - Kledmanee K AUID- ORCID: 0000-0001-7916-1365 AD - Department of Epidemiology, Faculty of Public Health, Mahidol University, Bangkok, Thailand. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLoS Negl Trop Dis JT - PLoS neglected tropical diseases JID - 101291488 SB - IM MH - Animals MH - Thailand/epidemiology MH - *Brucellosis/prevention & control/epidemiology MH - Humans MH - Cross-Sectional Studies MH - *Health Knowledge, Attitudes, Practice MH - Female MH - *Farmers/psychology/statistics & numerical data MH - Male MH - Surveys and Questionnaires MH - Livestock/microbiology MH - Adult MH - Middle Aged MH - *Zoonoses/epidemiology/prevention & control MH - One Health MH - Cattle PMC - PMC13712670 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 14:04 PHST- 2026/02/11 00:00 [received] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:04 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PNTD-D-26-00283 [pii] AID - 10.1371/journal.pntd.0014788 [doi] PST - epublish SO - PLoS Negl Trop Dis. 2026 Oct 27;20(10):e0014788. doi: 10.1371/journal.pntd.0014788. eCollection 2026 Oct. PMID- 42945352 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2053-1095 (Electronic) IS - 2053-1095 (Linking) VI - 12 IP - 6 DP - 2026 Nov TI - Comprehensive Ophthalmic Evaluation of Clinically Normal Rhesus Monkeys (Macaca mulatta): Selected Ophthalmic Diagnostic Tests, Tear-Film Biochemistry and Serum Correlations, Conjunctival Microbiota and Ophthalmic Artery Haemodynamics. LID - e71277 [pii] LID - 10.1002/vms3.71277 [doi] AB - BACKGROUND: The rhesus monkey (Macaca mulatta) is an important non-human primate model in ophthalmic research; however, comprehensive baseline data integrating clinical ophthalmic examinations, tear biochemistry, conjunctival microbiota and ophthalmic artery haemodynamics are limited. This study established normal ophthalmic baseline values and evaluated tear biochemical components and their correlations with serum concentrations in clinically healthy rhesus monkeys. METHODS: Thirty clinically healthy rhesus monkeys (3-11 years; both sexes) underwent comprehensive ophthalmic examination, including blink frequency, palpebral fissure length (PFL), Schirmer tear test-1 (STT-1), tear film breakup time (TBUT), intraocular pressure (IOP) in lateral and seated positions, ophthalmic artery colour Doppler ultrasonography and conjunctival bacterial culture. Tear and serum samples were analysed for albumin, glucose, creatinine, urea nitrogen, cholesterol and total protein. The effects of age and sex and correlations between tear and serum biochemical parameters were evaluated. RESULTS: Mean baseline values were: blink frequency 10.48 +/- 2.33 blinks/min, PFL 16.47 +/- 1.49 mm, STT-1 13.93 +/- 4.83 mm/min, TBUT 10.50 +/- 2.66 s, IOP 22.22 +/- 3.90 mmHg (lateral) and 17.34 +/- 3.38 mmHg (seated) and ophthalmic artery Doppler indices of PSV 41.65 +/- 3.57 cm/s, EDV 19.45 +/- 3.64 cm/s, PI 0.76 +/- 0.16 and RI 0.53 +/- 0.07. No significant differences were observed between eyes or sexes, whereas age significantly affected STT-1 and PFL. Conjunctival cultures were predominantly Gram-positive, with Staphylococcus spp. and Bacillus spp. most frequently isolated. Glucose and total protein differed significantly between tear and serum samples, while tear and serum glucose and urea concentrations were significantly correlated. CONCLUSIONS: These findings establish comprehensive ophthalmic, microbiological, haemodynamic and tear biochemical baseline values for healthy rhesus monkeys, providing a valuable baseline for clinical evaluation, early disease detection and comparative ophthalmic research. CI - (c) 2026 The Author(s). Veterinary Medicine and Science published by John Wiley & Sons Ltd. FAU - Davari, Ali AU - Davari A AUID- ORCID: 0009-0001-9594-9950 AD - Animal Core Facility, Reproductive Biomedicine Research Center, Royan Institute for Animal Biotechnology, ACECR, Tehran, Iran. FAU - Abarkar, Mohammad AU - Abarkar M AUID- ORCID: 0000-0003-2487-2164 AD - Department of Clinical Science, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Karaj, Iran. FAU - Maleki, Sajad AU - Maleki S AD - Department of Clinical Science, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran. FAU - Mirsadeghi, Ehsan AU - Mirsadeghi E AD - Animal Core Facility, Reproductive Biomedicine Research Center, Royan Institute for Animal Biotechnology, ACECR, Tehran, Iran. FAU - Tabandeh, Mohammad Reza AU - Tabandeh MR AUID- ORCID: 0000-0003-3258-8550 AD - Division of Biochemistry and Molecular Biology, Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran. AD - Stem Cells and Transgenic Technology Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran. FAU - Tamai, Iradj Ashrafi AU - Tamai IA AUID- ORCID: 0000-0001-6784-3721 AD - Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. FAU - Hajinasrollah, Mostafa AU - Hajinasrollah M AD - Animal Core Facility, Reproductive Biomedicine Research Center, Royan Institute for Animal Biotechnology, ACECR, Tehran, Iran. FAU - Dizaj, Ahmad Vosough Taghi AU - Dizaj AVT AD - Department of Reproductive Imaging, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran. LA - eng PT - Journal Article PL - England TA - Vet Med Sci JT - Veterinary medicine and science JID - 101678837 SB - IM MH - Animals MH - Female MH - *Macaca mulatta/physiology MH - *Conjunctiva/microbiology MH - *Tears/chemistry/physiology MH - Male MH - *Ophthalmic Artery/physiology MH - Hemodynamics MH - *Microbiota MH - *Diagnostic Techniques, Ophthalmological/veterinary MH - Reference Values PMC - PMC13712712 OTO - NOTNLM OT - Rhesus monkey OT - Schirmer tear test OT - colour Doppler imaging OT - conjunctival microbiota OT - intraocular pressure OT - tear film biochemistry OT - tear film breakup time COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 14:04 PHST- 2026/09/10 00:00 [revised] PHST- 2026/07/08 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:04 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e71277 [pii] AID - VMS371277 [pii] AID - 10.1002/vms3.71277 [doi] PST - ppublish SO - Vet Med Sci. 2026 Nov;12(6):e71277. doi: 10.1002/vms3.71277. PMID- 42945348 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Aberrant mRNA 5-methylcytosine modification contributes to ovarian dysfunction in a rat model of polycystic ovary syndrome. PG - e0358238 LID - 10.1371/journal.pone.0358238 [doi] LID - e0358238 AB - OBJECTIVE: This study investigated the altered landscape of messenger RNA 5-methylcytosine (m5C) methylation and the functional involvement of m5C-related regulators in a rat model of polycystic ovary syndrome (PCOS). METHODS: A PCOS rat model was established by dehydroepiandrosterone (DHEA) induction, with the phenotype confirmed through estrous cycle monitoring, serum sex hormone analysis, and ovarian morphological assessment. Differentially m5C-methylated mRNAs were identified using an m5C epitranscriptomic microarray, and their associated biological pathways were predicted by KEGG analysis. Global m5C methylation levels and the expression and m5C enrichment of selected pathway-related genes were validated in ovarian tissues and isolated primary ovarian granulosa cells using dot blot, RT-qPCR, Western blotting, and methylated RNA immunoprecipitation quantitative PCR (MeRIP-qPCR). The functional roles of TET2 and NSUN6 were further evaluated using shRNA-mediated knockdown, followed by analysis of PI3K/AKT-related signaling proteins, serum sex hormones, and ovarian histopathology. RESULTS: m5C modifications were predominantly localized within coding sequences and 3' untranslated regions. Among 1,090 RNAs showing both differential m5C methylation and expression, 36 genes exhibited significant correlations between methylation and expression changes. Global mRNA m5C methylation was significantly elevated in the PCOS group. The m5C-related regulators Nsun6 and Tet2 showed increased m5C enrichment and significantly elevated protein expression. KEGG analysis linked the differentially modified transcripts to endocrine resistance and insulin-related signaling pathways. Tet2, Nsun6, Src, Pi3k, and Akt transcripts exhibited increased m5C enrichment in PCOS ovarian tissues and primary granulosa cells. Concurrently, SRC and PI3K protein expression was increased, whereas AKT protein expression was decreased in the PCOS group. Knockdown of TET2 and NSUN6 was confirmed at the mRNA and protein levels and differentially modulated PI3K/AKT-related protein expression and phosphorylation status, as assessed by PI3K, p-PI3K, AKT, and p-AKT levels. TET2 and NSUN6 knockdown also partially improved PCOS-associated abnormalities in serum FSH, LH, the LH/FSH ratio, testosterone, estradiol, and ovarian morphology. CONCLUSION: This study reveals a significantly altered mRNA m5C methylation landscape in a DHEA-induced PCOS rat model and primary ovarian granulosa cells. The functional knockdown data suggest that TET2 and NSUN6 may contribute to PCOS-associated ovarian dysfunction through differential regulation or remodeling of PI3K/AKT signaling. However, the opposite changes in the p-PI3K/PI3K and p-AKT/AKT ratios indicate that the pathway is differentially regulated rather than uniformly activated or inhibited. Because the DHEA model primarily mimics hyperandrogenism and MeRIP-qPCR does not provide single-nucleotide resolution, further validation in human samples and high-resolution m5C mapping is required to establish the precise molecular mechanism and clinical relevance of these findings. CI - Copyright: (c) 2026 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Chen, Xiaohong AU - Chen X AUID- ORCID: 0000-0001-9108-5852 AD - Chengdu University of Traditional Chinese Medicine, Chengdu, China. FAU - Lin, Jing AU - Lin J AD - Guangxi University of Traditional Chinese Medicine, Nanning, China. FAU - Chi, Zhoulin AU - Chi Z AD - Guangxi University of Traditional Chinese Medicine, Nanning, China. FAU - Yang, Sha AU - Yang S AD - Chengdu University of Traditional Chinese Medicine, Chengdu, China. AD - Key Laboratory of Acupuncture for Senile Disease (Chengdu University of TCM), Ministry of Education, Chengdu, China. FAU - Yu, Shuguang AU - Yu S AD - Chengdu University of Traditional Chinese Medicine, Chengdu, China. AD - Acupoint Effects Key Laboratory of Sichuan Province, Chengdu, China. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 6R795CQT4H (5-Methylcytosine) RN - 0 (RNA, Messenger) RN - EC 2.7.1.- (Phosphatidylinositol 3-Kinases) RN - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) SB - IM MH - Animals MH - Female MH - *Polycystic Ovary Syndrome/genetics/metabolism/pathology/physiopathology/chemically induced MH - *5-Methylcytosine/metabolism MH - Rats MH - *RNA, Messenger/metabolism/genetics MH - RNA Methylation MH - Disease Models, Animal MH - *Ovary/metabolism/physiopathology/pathology MH - Granulosa Cells/metabolism MH - Rats, Sprague-Dawley MH - Epitranscriptome MH - Signal Transduction MH - Phosphatidylinositol 3-Kinases/metabolism MH - Proto-Oncogene Proteins c-akt/metabolism MH - Epitranscriptomics PMC - PMC13712706 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 14:03 PHST- 2026/03/01 00:00 [received] PHST- 2026/08/30 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:03 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PONE-D-26-10382 [pii] AID - 10.1371/journal.pone.0358238 [doi] PST - epublish SO - PLoS One. 2026 Oct 27;21(10):e0358238. doi: 10.1371/journal.pone.0358238. eCollection 2026. PMID- 42945338 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 2767-3375 (Electronic) IS - 2767-3375 (Linking) VI - 6 IP - 10 DP - 2026 TI - Causes of stillbirth in sub-Saharan Africa and South Asia: Findings from Child Health and Mortality Prevention Surveillance, 2016-2023. PG - e0005235 LID - 10.1371/journal.pgph.0005235 [doi] LID - e0005235 AB - BACKGROUND: Globally, an estimated 1.9 million stillbirths occur annually, yet significant knowledge gaps exist regarding the causes of stillbirths, particularly in high-burden regions. We investigated fetal and maternal conditions causing stillbirths in seven countries in sub-Saharan Africa and South Asia and described missed opportunities for prevention. METHODS: Child Health and Mortality Prevention Surveillance (CHAMPS) identified stillbirths at sites in Bangladesh, Ethiopia, Kenya, Mali, Mozambique, Sierra Leone, and South Africa. We asked families for consent to conduct minimally invasive tissue sampling (MITS) from December 2016 to December 2023. An expert panel reviewed test results, clinical information, and verbal autopsy data and assigned fetal and maternal conditions leading to stillbirths and identified missed opportunities for prevention. FINDINGS: A cause of death (fetal or maternal condition) was determined for 94% (2342/2492) of stillbirths; the most frequent condition in the fetus was intrauterine hypoxia (75%, 1864/2492) across all sites, resulting from either maternal condition (49%, 922/1864) or placental causes (33%, 610/1864). Congenital infections were determined to be the cause of 9% (228/2492) of stillbirths, accounting for the highest proportion in South Africa (31%, 96/306). Group B Streptococcus, Escherichia coli, and Enterococcus faecalis were the most common causative pathogens. Congenital birth defects caused 9% (227/2492) overall and were most common in Ethiopia (24%, 136/568). Primary maternal conditions were identified in 72%, most often placental complications (18%, 446/2492) and maternal hypertension (17%, 414/2492). Complications of placenta, cord and membranes were more common in Mali (43%, 92/212) while maternal medical and surgical conditions were most frequently observed in South Africa (40%, 121/306) and Bangladesh (39%, 158/405). Most (72%, 1808/2492) causes of stillbirth were considered preventable, with heterogeneity observed across sites on the recommended prevention strategies. INTERPRETATION: Complications of pregnancy or delivery accounted for most stillbirths across all sites, with intrauterine hypoxia identified as the predominant fetal condition highlighting widespread and persistent gaps in the timely identification and management of maternal and placental conditions during antenatal and obstetric care across sites in Africa and South Asia. At the same time, substantial geographic variation in causes and prevention opportunities, including for infections and birth defects, underscored the need for locally tailored strategies. Strengthening facility-based care alongside targeted interventions based on site-specific patterns, will be critical to reducing the stillbirth burden in these high-priority regions. FUNDING: The Gates Foundation. CI - Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. FAU - Rahman, Afruna AU - Rahman A AD - International Center for Diarrhoeal Diseases Research (icddr, b), Dhaka, Bangladesh. FAU - Lee, Kyu Han AU - Lee KH AD - Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, United States of America. AD - Emory Global Health Institute, Emory University, Atlanta, Georgia, United States of America. FAU - Arifeen, Shams El AU - Arifeen SE AD - International Center for Diarrhoeal Diseases Research (icddr, b), Dhaka, Bangladesh. FAU - Hossain, Mohammad Zahid AU - Hossain MZ AD - International Center for Diarrhoeal Diseases Research (icddr, b), Dhaka, Bangladesh. FAU - Chowdhury, Atique Iqbal AU - Chowdhury AI AD - International Center for Diarrhoeal Diseases Research (icddr, b), Dhaka, Bangladesh. FAU - Alam, Muntasir AU - Alam M AD - International Center for Diarrhoeal Diseases Research (icddr, b), Dhaka, Bangladesh. FAU - Afrin, Afsana AU - Afrin A AUID- ORCID: 0009-0006-5108-9801 AD - International Center for Diarrhoeal Diseases Research (icddr, b), Dhaka, Bangladesh. FAU - Ogbuanu, Ikechukwu Udo AU - Ogbuanu IU AD - The Africa Research Collaborative (the ARC), Freetown, Sierra Leone. FAU - Samura, Solomon AU - Samura S AD - World Hope International, Makeni, Sierra Leone. FAU - Kaluma, Erick AU - Kaluma E AUID- ORCID: 0009-0009-6416-6724 AD - The Africa Research Collaborative (the ARC), Freetown, Sierra Leone. FAU - Ojulong, Julius AU - Ojulong J AUID- ORCID: 0000-0003-2200-8954 AD - The Africa Research Collaborative (the ARC), Freetown, Sierra Leone. FAU - Sow, Samba AU - Sow S AD - Centre pour le Developpement des Vaccins (CVD-Mali), Ministere de la Sante, Bamako, Mali. FAU - Keita, Adama Mamby AU - Keita AM AD - Centre pour le Developpement des Vaccins (CVD-Mali), Ministere de la Sante, Bamako, Mali. FAU - Tapia, Milagritos D AU - Tapia MD AD - Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, United States of America. FAU - Chawla, Kiranpreet Kaur AU - Chawla KK AD - Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, United States of America. FAU - Kotloff, Karen AU - Kotloff K AD - Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, United States of America. FAU - Madhi, Shabir A AU - Madhi SA AD - South African Medical Research Council Vaccines and Infectious Diseases Analytics (VIDA) Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. AD - Faculty of Health Sciences, Wits Infectious Disease and Oncology Research Institute, University of the Witwatersrand, Johannesburg, South Africa. FAU - Mahtab, Sana AU - Mahtab S AD - South African Medical Research Council Vaccines and Infectious Diseases Analytics (VIDA) Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. FAU - Adam, Yasmin AU - Adam Y AD - South African Medical Research Council Vaccines and Infectious Diseases Analytics (VIDA) Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. FAU - Wise, Amy AU - Wise A AUID- ORCID: 0000-0001-5182-9060 AD - Wits Department of Obstetrics & Gynaecology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. FAU - Dangor, Ziyaad AU - Dangor Z AD - South African Medical Research Council Vaccines and Infectious Diseases Analytics (VIDA) Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. AD - Faculty of Health Sciences, Wits Infectious Disease and Oncology Research Institute, University of the Witwatersrand, Johannesburg, South Africa. FAU - Mugah, Christopher AU - Mugah C AD - Kenya Medical Research Institute (KEMRI), Kisumu, Kenya. FAU - Oele, Elizabeth AU - Oele E AD - Kisumu County of Department of Health, Kisumu, Kenya. FAU - Omore, Richard AU - Omore R AUID- ORCID: 0000-0003-3702-3030 AD - Kenya Medical Research Institute (KEMRI), Kisumu, Kenya. FAU - Igunza, Aggrey K AU - Igunza AK AUID- ORCID: 0000-0002-5841-5431 AD - Kenya Medical Research Institute (KEMRI), Kisumu, Kenya. FAU - Onyango, Dickens AU - Onyango D AD - Kisumu County of Department of Health, Kisumu, Kenya. FAU - Mandomando, Inacio AU - Mandomando I AD - Centro de Investigacao em Saude de Manhica [CISM], Maputo, Mozambique. AD - Instituto Nacional de Saude [INS], Maputo, Mozambique. AD - ISGlobal - Hospital Clinic, Universitat de Barcelona, Barcelona, Spain. FAU - Bassat, Quique AU - Bassat Q AUID- ORCID: 0000-0003-0875-7596 AD - Centro de Investigacao em Saude de Manhica [CISM], Maputo, Mozambique. AD - ISGlobal - Hospital Clinic, Universitat de Barcelona, Barcelona, Spain. AD - Instituto Catalana de Recerca i Estudis Avancats [ICREA], Barcelona, Spain. AD - Pediatrics Department, Hospital Sant Joan de Deu, Universitat de Barcelona, Esplugues, Barcelona, Spain. AD - CIBER de Epidemiologia y Salud Publica, Instituto de Salud Carlos III, Madrid, Spain. FAU - Ajanovic, Sara AU - Ajanovic S AD - Centro de Investigacao em Saude de Manhica [CISM], Maputo, Mozambique. AD - ISGlobal - Hospital Clinic, Universitat de Barcelona, Barcelona, Spain. FAU - Varo, Rosauro AU - Varo R AUID- ORCID: 0000-0003-4282-5572 AD - Centro de Investigacao em Saude de Manhica [CISM], Maputo, Mozambique. AD - ISGlobal - Hospital Clinic, Universitat de Barcelona, Barcelona, Spain. FAU - Xerinda, Elisio AU - Xerinda E AD - ISGlobal - Hospital Clinic, Universitat de Barcelona, Barcelona, Spain. FAU - Assefa, Nega AU - Assefa N AD - College of Health and Medical Sciences, Haramaya University, Harar, Ethiopia. FAU - Scott, J Anthony G AU - Scott JAG AUID- ORCID: 0000-0001-7533-5006 AD - Department of Infectious Disease Epidemiology and International Health, London School of Hygiene & Tropical Medicine, London, United Kingdom. FAU - Madrid, Lola AU - Madrid L AD - College of Health and Medical Sciences, Haramaya University, Harar, Ethiopia. AD - Department of Infectious Disease Epidemiology and International Health, London School of Hygiene & Tropical Medicine, London, United Kingdom. AD - Ayder Specialized Comprehensive Hospital, Mekelle University, Mekele, Ethiopia. AD - Liverpool School of Tropical Medicine (LSTM), Liverpool, England. FAU - Yeshi, Melisachew M AU - Yeshi MM AD - Ayder Specialized Comprehensive Hospital, Mekelle University, Mekele, Ethiopia. FAU - Temesgen, Fikremelekot AU - Temesgen F AUID- ORCID: 0000-0001-7927-2153 AD - Addis Ababa University, Addis Ababa, Ethiopia. FAU - Liu, Lucy AU - Liu L AD - Emory Global Health Institute, Emory University, Atlanta, Georgia, United States of America. FAU - Mutevedzi, Portia AU - Mutevedzi P AD - Emory Global Health Institute, Emory University, Atlanta, Georgia, United States of America. FAU - Blau, Dianna M AU - Blau DM AUID- ORCID: 0000-0002-1253-198X AD - Center for Global Health, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America. FAU - Akelo, Victor AU - Akelo V AD - Kenya Medical Research Institute (KEMRI), Kisumu, Kenya. AD - Liverpool School of Tropical Medicine (LSTM), Liverpool, England. FAU - Breiman, Robert F AU - Breiman RF AD - Emory Global Health Institute, Emory University, Atlanta, Georgia, United States of America. FAU - Whitney, Cynthia G AU - Whitney CG AUID- ORCID: 0000-0002-1056-3216 AD - Emory Global Health Institute, Emory University, Atlanta, Georgia, United States of America. FAU - Gurley, Emily S AU - Gurley ES AUID- ORCID: 0000-0002-8648-9403 AD - International Center for Diarrhoeal Diseases Research (icddr, b), Dhaka, Bangladesh. AD - Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, United States of America. CN - CHAMPS Consortium LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLOS Glob Public Health JT - PLOS global public health JID - 9918283779606676 PMC - PMC13712675 COIS- CGW reports other grants from the Bill & Melinda Gates Foundation for work on malnutrition, HIV, and Covid vaccines, other than the submitted work. AW reports being a council member of the national Obstetrician and & Gynaecologists council. KK reports grant from the Bill & Melinda Gates Foundation and support for attending meetings. SEA reports his employment at icddr,b, an organization that receives grants for related projects and funding from WHO & BMGF to support his travels. All other authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 14:03 PHST- 2025/09/08 00:00 [received] PHST- 2026/08/04 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:03 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PGPH-D-25-02617 [pii] AID - 10.1371/journal.pgph.0005235 [doi] PST - epublish SO - PLOS Glob Public Health. 2026 Oct 27;6(10):e0005235. doi: 10.1371/journal.pgph.0005235. eCollection 2026. PMID- 42945328 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1937-9145 (Electronic) IS - 1945-0877 (Linking) VI - 19 IP - 961 DP - 2026 Oct 27 TI - Disruption of the N-terminal domain in a STAT3 hyper-IgE syndrome variant causes disease by sequestering STAT3 in the cytoplasm. PG - eaeg5302 LID - 10.1126/scisignal.aeg5302 [doi] AB - Autosomal-dominant hyper-IgE syndrome (HIES) is characterized by chronic mucocutaneous candidiasis, recurrent sinopulmonary complications, noninflammatory atopic dermatitis, high amounts of serum IgE, and nonimmunological manifestations affecting the musculoskeletal and vascular systems. Most cases are caused by heterozygous loss-of-function, dominant-negative (LOF/DN) variants that affect the DNA binding, SH2, or transactivation domains of STAT3. Here, we report a patient with a unique STAT3 allele affecting the STAT3 N-terminal domain (R31Q), who presented with disseminated invasive aspergillosis affecting the brain and lung, bronchiectasis, and high serum IgE but lacked nonimmune disease manifestations. Biochemical analyses in cell lines demonstrated that R31Q reduced the abundance and transcriptional activity of STAT3 and caused it to exhibit negative dominance on wild-type (WT) STAT3. This was due to preferential STAT3(R31Q) dimerization with STAT3(WT), sequestration of STAT3(WT):STAT3(R31Q) dimers in the cytosol, and a reduction in STAT3 nuclear translocation and transcriptional activity. Lymphocytes from the patient exhibited some typical defects observed in canonical STAT3(LOF/DN) HIES, such as reduced memory B cells, MAIT cells, and T(H)17 cytokines. However, naive B cells from this patient had only a partial impairment in immunoglobulin secretion induced by interleukin-21 (IL-21) in vitro, and the patient had normal numbers of T(H)2 cells and circulating T(FH) cells. Our findings reveal that the N-terminal domain regulates STAT3 dimer formation and function and provide a previously unknown biochemical mechanism of disease pathogenesis in STAT3(LOF/DN) HIES. FAU - Mackie, Joseph AU - Mackie J AUID- ORCID: 0000-0002-6571-4560 AD - Garvan Institute of Medical Research, Darlinghurst, Australia. AD - School of Clinical Medicine, Faculty of Medicine and Health, UNSW Sydney, Sydney, Australia. AD - Clinical Immunogenomics Research Consortium Australasia (CIRCA), Sydney, Australia. FAU - Suan, Dan AU - Suan D AUID- ORCID: 0000-0001-5996-5120 AD - Garvan Institute of Medical Research, Darlinghurst, Australia. AD - School of Clinical Medicine, Faculty of Medicine and Health, UNSW Sydney, Sydney, Australia. AD - Clinical Immunogenomics Research Consortium Australasia (CIRCA), Sydney, Australia. FAU - Langley, David B AU - Langley DB AUID- ORCID: 0000-0003-2752-0041 AD - Garvan Institute of Medical Research, Darlinghurst, Australia. AD - School of Clinical Medicine, Faculty of Medicine and Health, UNSW Sydney, Sydney, Australia. FAU - Avery, Danielle T AU - Avery DT AUID- ORCID: 0000-0002-9156-0489 AD - Garvan Institute of Medical Research, Darlinghurst, Australia. FAU - Rao, Geetha AU - Rao G AD - Garvan Institute of Medical Research, Darlinghurst, Australia. FAU - Freeman, Alexandra F AU - Freeman AF AUID- ORCID: 0000-0001-7005-8345 AD - National Institute of Allergy and Infectious Disease (NIAID), Bethesda, MD, USA. FAU - Gray, Paul E AU - Gray PE AD - Clinical Immunogenomics Research Consortium Australasia (CIRCA), Sydney, Australia. AD - Department of Immunology and Infectious Disease, Sydney Children's Hospital, Randwick, Australia. AD - School of Women's and Children's Health, UNSW Sydney, Sydney, Australia. FAU - Gracias Flor, Francis AU - Gracias Flor F AUID- ORCID: 0009-0005-4323-7440 AD - Garvan Institute of Medical Research, Darlinghurst, Australia. FAU - Okada, Satoshi AU - Okada S AUID- ORCID: 0000-0002-4622-5657 AD - Department of Pediatrics, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan. FAU - Christ, Daniel AU - Christ D AUID- ORCID: 0000-0002-7313-3977 AD - Garvan Institute of Medical Research, Darlinghurst, Australia. AD - School of Clinical Medicine, Faculty of Medicine and Health, UNSW Sydney, Sydney, Australia. FAU - Croucher, David R AU - Croucher DR AUID- ORCID: 0000-0003-4965-8674 AD - Garvan Institute of Medical Research, Darlinghurst, Australia. AD - School of Clinical Medicine, Faculty of Medicine and Health, UNSW Sydney, Sydney, Australia. FAU - Guerin, Antoine AU - Guerin A AUID- ORCID: 0000-0001-6810-1607 AD - Garvan Institute of Medical Research, Darlinghurst, Australia. AD - School of Clinical Medicine, Faculty of Medicine and Health, UNSW Sydney, Sydney, Australia. AD - Clinical Immunogenomics Research Consortium Australasia (CIRCA), Sydney, Australia. FAU - Ma, Cindy S AU - Ma CS AUID- ORCID: 0000-0001-5387-8413 AD - Garvan Institute of Medical Research, Darlinghurst, Australia. AD - School of Clinical Medicine, Faculty of Medicine and Health, UNSW Sydney, Sydney, Australia. AD - Clinical Immunogenomics Research Consortium Australasia (CIRCA), Sydney, Australia. FAU - Tangye, Stuart G AU - Tangye SG AUID- ORCID: 0000-0002-5360-5180 AD - Garvan Institute of Medical Research, Darlinghurst, Australia. AD - School of Clinical Medicine, Faculty of Medicine and Health, UNSW Sydney, Sydney, Australia. AD - Clinical Immunogenomics Research Consortium Australasia (CIRCA), Sydney, Australia. LA - eng PT - Case Reports PT - Journal Article DEP - 20261027 PL - United States TA - Sci Signal JT - Science signaling JID - 101465400 RN - MKM3CA6LT1 (Interleukin-21) RN - 0 (Interleukins) RN - 0 (STAT3 protein, human) RN - 0 (STAT3 Transcription Factor) SB - IM MH - Humans MH - Male MH - *Cytoplasm/metabolism/genetics MH - Interleukin-21 MH - Interleukins/metabolism MH - *Job Syndrome/genetics/metabolism/pathology MH - Protein Domains MH - *STAT3 Transcription Factor/genetics/metabolism/chemistry MH - Young Adult EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 14:02 PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:02 [entrez] AID - 10.1126/scisignal.aeg5302 [doi] PST - ppublish SO - Sci Signal. 2026 Oct 27;19(961):eaeg5302. doi: 10.1126/scisignal.aeg5302. Epub 2026 Oct 27. PMID- 42945327 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1937-9145 (Electronic) IS - 1945-0877 (Linking) VI - 19 IP - 961 DP - 2026 Oct 27 TI - Neuronal tRNA modifications in Aplysia californica are repatterned during behavioral habituation. PG - eaed9028 LID - 10.1126/scisignal.aed9028 [doi] AB - Transfer RNA (tRNA) modifications are increasingly implicated as posttranscriptional regulators of basic neuronal functions. Characterizing tRNA modification profiles in specific neural circuits that control well-defined behaviors is challenging because of the complexity of conventional neurobiological models. Here, we leveraged the numerically simple central nervous system (CNS) of the marine mollusk Aplysia californica to investigate tRNA modification dynamics during habituation of a defensive reflex. We identified and quantified dozens of tRNA modifications and characterized the distribution of select small RNA modifications across different neuronal and nonneuronal tissues. Upon behavioral habituation of the siphon-elicited siphon withdrawal reflex (SSWR), we found that tRNA modification profiles in the major ganglion that controls the SSWR were repatterned with predictable, learning-related changes. We found a family of anticodon loop modifications that changed with habituation learning, including N(6)-isopentenyladenosine (i(6)A), which increased in abundance, and additional modifications N(1)-methyladenosine (m(1)A) and N(7)-methylguanosine (m(7)G), which decreased. These tRNA modification dynamics occurred independently of changes in the expression of their parent tRNAs, illustrating that behavioral habituation altered the activity of tRNA-modifying enzymes. Overall, our work reveals a link between tRNA modifications and behavioral habituation and provides insights toward understanding the posttranscriptional mechanisms of learning and memory. FAU - Huang, Han Xin AU - Huang HX AUID- ORCID: 0009-0003-0698-3562 AD - Department of Chemistry, Tufts University, Medford, MA 02155, USA. FAU - Floro, Gabriella M AU - Floro GM AUID- ORCID: 0009-0009-2096-3115 AD - Department of Chemistry, Tufts University, Medford, MA 02155, USA. FAU - Asare, Emmanuel AU - Asare E AD - Department of Chemistry, Tufts University, Medford, MA 02155, USA. FAU - Huang, Weichen AU - Huang W AUID- ORCID: 0000-0001-7870-3476 AD - Department of Chemistry, Tufts University, Medford, MA 02155, USA. FAU - Clark, Kevin D AU - Clark KD AUID- ORCID: 0000-0002-0576-7716 AD - Department of Chemistry, Tufts University, Medford, MA 02155, USA. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Sci Signal JT - Science signaling JID - 101465400 RN - 9014-25-9 (RNA, Transfer) SB - IM MH - Animals MH - *Aplysia/genetics/physiology/metabolism MH - *RNA, Transfer/genetics/metabolism MH - *Habituation, Psychophysiologic/physiology MH - *Neurons/metabolism MH - *RNA Processing, Post-Transcriptional MH - *Behavior, Animal/physiology MH - RNA Methylation EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 14:02 PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 14:02 [entrez] AID - 10.1126/scisignal.aed9028 [doi] PST - ppublish SO - Sci Signal. 2026 Oct 27;19(961):eaed9028. doi: 10.1126/scisignal.aed9028. Epub 2026 Oct 27. PMID- 42945325 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2053-1095 (Electronic) IS - 2053-1095 (Linking) VI - 12 IP - 6 DP - 2026 Nov TI - Chondroprotective Effect of PCSO-524 (Green-Lipped Mussel Extraction) Application Evaluated by Ultrasonography in Pomeranian and Chihuahua With Medial Patellar Luxation Grade II. LID - e71304 [pii] LID - 10.1002/vms3.71304 [doi] AB - OBJECTIVE: This research evaluated the chondroprotective efficacy of PCSO-524 in dogs with osteoarthritis (OA) secondary to medial patellar luxation (MPL) using ultrasonography - a non-invasive technique recommended for early OA detection. MATERIALS AND METHODS: Animals: 25 dogs total (21 with MPL grade II, 50 stifle joints evaluated) divided into three groups, including control group: 10 dogs receiving placebo, treatment group: 11 dogs receiving PCSO-524 (50 mg daily) and normal group: 4 healthy dogs (reference) Duration: 16 weeks Evaluations: Lameness scores, blood profiles, ultrasonographic findings, radiographic assessments and owner questionnaires at baseline, weeks 2, 4, 8, 12 and 16. RESULTS: The treatment group showed significant improvement in lameness scores within 8 weeks. The cartilage lesions improved within 4 weeks of PCSO-524 administration. Synovial fluid scores decreased significantly by week 4 in the treatment group. Radiographic OA scores improved mildly at 12 weeks in the treatment group versus progression in the control group. Owners observed better gait and quality of life by week 2. No adverse effects on blood chemistry or organ function. CONCLUSION: PCSO-524 demonstrates effective cartilage protection in dogs with OA secondary to MPL, with improvements in clinical signs, ultrasonographic findings and radiographic outcomes. For dogs that cannot undergo surgical correction, PCSO-524 supplementation combined with quadriceps muscle training and environmental management is recommended for improved quality of life. CI - (c) 2026 The Author(s). Veterinary Medicine and Science published by John Wiley & Sons Ltd. FAU - Soontornvipart, Kumpanart AU - Soontornvipart K AUID- ORCID: 0000-0002-6226-9000 AD - Department of Veterinary Surgery, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand. FAU - Sathienbumrungkit, Ratthanan AU - Sathienbumrungkit R AD - Department of Veterinary Surgery, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand. AD - Faculty of Veterinary Science, Small Animal Hospital, Chulalongkorn University, Bangkok, Thailand. LA - eng PT - Journal Article PL - England TA - Vet Med Sci JT - Veterinary medicine and science JID - 101678837 SB - IM MH - Animals MH - Dogs MH - Ultrasonography/veterinary MH - *Dog Diseases/drug therapy/diagnostic imaging/etiology MH - *Patellar Dislocation/veterinary/complications/diagnostic imaging/drug therapy MH - Female MH - *Osteoarthritis/veterinary/drug therapy/etiology/diagnostic imaging MH - Male MH - Lameness, Animal MH - Stifle/diagnostic imaging PMC - PMC13712575 OTO - NOTNLM OT - PCSO-524 OT - medial patellar luxation OT - ultrasound COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. This clinical trial was conducted independently, and the evaluation of all ultrasonographic, radiographic and clinical outcomes was completed without financial or material bias from any commercial entity. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:55 PHST- 2026/09/09 00:00 [revised] PHST- 2026/07/13 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:55 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e71304 [pii] AID - VMS371304 [pii] AID - 10.1002/vms3.71304 [doi] PST - ppublish SO - Vet Med Sci. 2026 Nov;12(6):e71304. doi: 10.1002/vms3.71304. PMID- 42945312 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Understanding the processes involved in sepsis recognition across clinical contexts to guide individualised management: A phenomenographic study. PG - e0360913 LID - 10.1371/journal.pone.0360913 [doi] LID - e0360913 AB - BACKGROUND: Sepsis is a highly heterogeneous condition, yet it is commonly recognised by application of standardised criteria, irrespective of clinical context. Understanding the complexity of sepsis recognition in the heterogeneous populations of patients with sepsis is critical to better target our resources to the needs of patients. Our aim was to examine nurses' clinical experiences to understand the processes involved in sepsis recognition across clinical contexts. METHODS: Semi-structured interviews were conducted with 26 nurses with various levels of experience in caring for patients with sepsis from multiple hospital settings. The interviews were phenomenographically analysed using the Arkelind method. The Cynefin framework was used to inform the organisation of findings according to contextual complexity. FINDINGS: We identified four categories of description that represent different ways of experiencing sepsis recognition: recognise sepsis by following protocol-based care, recognise sepsis by following disease-specific care, recognise sepsis according to emergent care, and recognise sepsis according to emergency care. The categories emerged from various clinical contexts, ranging from the simple to the highly complex. This variability directed clinical management by helping nurses decide whether guidelines apply to the individual patient and how they should be integrated into clinical decision-making. CONCLUSIONS: Despite its apparent simplicity, sepsis recognition is complex and context-dependent. Rather than relying on an identical strategy for all patients, effective clinical decisions require personalisation based on the characteristics of the clinical contexts. Our findings show that considering the availability of data, predictability and cause-and-effect relationships can inform the selection of an appropriate approach to sepsis recognition. This approach demands adaptability; however, unless we adopt an approach that is appropriate to the level of clinical complexity, significant improvements in sepsis recognition may not be seen. Prospective research is required to validate our findings. CI - Copyright: (c) 2026 Nemeckova et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Nemeckova, Marika AU - Nemeckova M AUID- ORCID: 0009-0001-1895-254X AD - Department of Nursing, Midwifery and Health, Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne, United Kingdom. FAU - Conner, Tony AU - Conner T AD - Department of Nursing, Midwifery and Health, Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne, United Kingdom. FAU - Steven, Alison AU - Steven A AUID- ORCID: 0000-0003-4811-1706 AD - Department of Nursing, Midwifery and Health, Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne, United Kingdom. AD - Department of Health Sciences, School of Medical and Pharmaceutical Sciences, University of Genova, Genova, Italy. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Humans MH - *Sepsis/diagnosis/therapy/nursing MH - Female MH - Adult MH - Male MH - Clinical Decision-Making MH - Nurses PMC - PMC13712736 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:55 PHST- 2026/01/14 00:00 [received] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:55 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PONE-D-26-01944 [pii] AID - 10.1371/journal.pone.0360913 [doi] PST - epublish SO - PLoS One. 2026 Oct 27;21(10):e0360913. doi: 10.1371/journal.pone.0360913. eCollection 2026. PMID- 42945307 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1545-7885 (Electronic) IS - 1544-9173 (Print) IS - 1544-9173 (Linking) VI - 24 IP - 10 DP - 2026 Oct TI - Pollinator abundance shapes sexual selection in an angiosperm. PG - e3003579 LID - 10.1371/journal.pbio.3003579 [doi] LID - e3003579 AB - Sexual selection is a central force in evolutionary biology that has been shown to shape phenotypes and population dynamics in animals. While this selective force is increasingly recognized in flowering plants, theory predicts that anisogamy generates sex differences in the fitness benefits of acquiring additional mates-a pattern well supported in animals but with limited tests in plants. Plant studies rely solely on genetic parentage analyses to estimate mate numbers, thereby conflating performance in accessing mates with success in gamete fertilization. Pollinators add an ecological layer, as their behavior and abundance can influence competition for access to mates and their gametes. Here, we disentangle consecutive episodes of sexual selection in the hermaphroditic plant Brassica rapa by combining direct observations of Bombus terrestris movements with genetic paternity analyses in experimental populations, thus providing independent estimates of the observational number of mates acquired and realized fertilization success. Our approach identifies pollen competition for ovules as the primary arena for sexual selection in B. rapa. Competition for access to mates constitutes a secondary force and was found to be stronger in the male compared to the female sex function, as predicted by classic theory. Experimentally reduced pollinator abundance modulated the balance between pre- and post-pollination sexual selection, increasing the opportunity for selection on mate acquisition. Ignoring pollinator movements led to biased estimates of pre-pollination sexual selection. Our findings demonstrate that sexual selection in plants unfolds across multiple, sequential episodes shaped by ecological context. CI - Copyright: (c) 2026 Barbot et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Barbot, Estelle AU - Barbot E AUID- ORCID: 0000-0001-6790-2429 AD - ISEM, University of Montpellier, CNRS, IRD, Montpellier, France. FAU - Dufay, Mathilde AU - Dufay M AD - CEFE, University of Montpellier, CNRS, EPHE, IRD, Montpellier, France. FAU - Janicke, Tim AU - Janicke T AD - CEFE, University of Montpellier, CNRS, EPHE, IRD, Montpellier, France. FAU - Lapeyre, Benoit AU - Lapeyre B AD - CEFE, University of Montpellier, CNRS, EPHE, IRD, Montpellier, France. FAU - Orcel, Denis AU - Orcel D AD - ISEM, University of Montpellier, CNRS, IRD, Montpellier, France. FAU - Rousset, Francois AU - Rousset F AD - ISEM, University of Montpellier, CNRS, IRD, Montpellier, France. FAU - Tonnabel, Jeanne AU - Tonnabel J AUID- ORCID: 0000-0003-3461-6965 AD - ISEM, University of Montpellier, CNRS, IRD, Montpellier, France. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLoS Biol JT - PLoS biology JID - 101183755 SB - IM MH - Animals MH - *Pollination/physiology MH - Bees/physiology MH - *Sexual Selection MH - *Brassica rapa/physiology/genetics MH - Pollen/physiology MH - Ovule/physiology MH - Fertilization PMC - PMC13712744 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:55 PHST- 2025/09/12 00:00 [received] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:55 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PBIOLOGY-D-25-02966 [pii] AID - 10.1371/journal.pbio.3003579 [doi] PST - epublish SO - PLoS Biol. 2026 Oct 27;24(10):e3003579. doi: 10.1371/journal.pbio.3003579. eCollection 2026 Oct. PMID- 42945304 OWN - NLM STAT- Publisher LR - 20261027 IS - 1538-7445 (Electronic) IS - 0008-5472 (Linking) DP - 2026 Oct 27 TI - Targeting Branched-Chain Amino Acid Transamination Sensitizes Clear Cell Renal Cell Carcinoma to Glutaminase Inhibition. LID - 10.1158/0008-5472.CAN-26-0563 [doi] AB - Metabolic reprogramming is a well-described hallmark of clear cell renal cell carcinoma (ccRCC). Gene copy number variation and altered gene expression data suggest enhanced branched-chain amino acid (BCAA) transamination and reduced BCAA oxidation are highly selected traits in ccRCC. Surprisingly, we found that genetic modulation of BCAA oxidation had little effect on experimental ccRCC cell growth in vitro and in vivo. In contrast, BCAA transamination in ccRCC cells was essential for the generation of glutamate-derived glutathione and cell viability when glutamine was limiting. Thus, BCAA transamination conferred resistance to states of low glutamine or treatment with the glutaminase inhibitor CB-839 by providing glutathione independent of glutamine breakdown. Targeting BCAA transamination in vitro and in vivo sensitized ccRCC to CB-839 mediated glutaminase inhibition by decreasing glutathione biosynthesis and redox homeostasis. These findings reveal that BCAA transamination maintains critical glutamate and glutathione levels in glutamine-deprived ccRCC, explaining why CB-839 treatment has been ineffective in ccRCC clinical trials and demonstrate an approach to treat ccRCC by targeting BCAA transamination to create a vulnerability to glutaminase inhibition. FAU - Coffey, Nathan J AU - Coffey NJ AUID- ORCID: 0000-0002-9413-5533 AD - University of Pennsylvania Philadelphia, PA United States. ROR: https://ror.org/00b30xv10 FAU - Demczyszyn, Sara M AU - Demczyszyn SM AUID- ORCID: 0009-0002-4761-8644 AD - University of Pennsylvania Philadelphia, PA United States. ROR: https://ror.org/00b30xv10 FAU - Xue, Yizheng AU - Xue Y AUID- ORCID: 0009-0000-3255-3150 AD - University of Pennsylvania Philadelphia, PA United States. ROR: https://ror.org/00b30xv10 FAU - Zhang, Zian AU - Zhang Z AUID- ORCID: 0009-0001-9459-5791 AD - University of Pennsylvania United States. FAU - Skuli, Nicolas AU - Skuli N AUID- ORCID: 0000-0002-1654-2467 AD - University of Pennsylvania Philadelphia, PA United States. ROR: https://ror.org/00b30xv10 FAU - Lesner, Nicholas P AU - Lesner NP AUID- ORCID: 0000-0001-9734-8828 AD - University of Pennsylvania United States. ROR: https://ror.org/00b30xv10 FAU - Riscal, Romain AU - Riscal R AUID- ORCID: 0000-0001-8969-224X AD - Inserm Montpellier France. ROR: https://ror.org/02vjkv261 FAU - Jiang, Yanqing AU - Jiang Y AUID- ORCID: 0000-0003-1240-0784 AD - University of Pennsylvania United States. ROR: https://ror.org/00b30xv10 FAU - Han, Xu AU - Han X AUID- ORCID: 0000-0001-5663-2435 AD - University of Pennsylvania United States. ROR: https://ror.org/00b30xv10 FAU - Carens, Madeleine AU - Carens M AUID- ORCID: 0000-0002-9142-7522 AD - University of Pennsylvania Philadelphia, PA United States. ROR: https://ror.org/00b30xv10 FAU - Brown, Morgan AU - Brown M AUID- ORCID: 0000-0002-8569-7913 AD - University of Pennsylvania Philadelphia, PA United States. ROR: https://ror.org/00b30xv10 FAU - Kim, Laura C AU - Kim LC AUID- ORCID: 0000-0001-8229-2745 AD - University of Pennsylvania Philadelphia, PA United States. ROR: https://ror.org/00b30xv10 FAU - Keith, Brian AU - Keith B AUID- ORCID: 0000-0002-4853-0815 AD - University of Pennsylvania Philadelphia, PA United States. ROR: https://ror.org/00b30xv10 FAU - Arany, Zoltan AU - Arany Z AUID- ORCID: 0000-0003-1368-2453 AD - University of Pennsylvania Philadelphia United States. ROR: https://ror.org/00b30xv10 FAU - Simon, M Celeste AU - Simon MC AUID- ORCID: 0000-0001-9106-447X AD - University of Pennsylvania Philadelphia, PA United States. ROR: https://ror.org/00b30xv10 LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Cancer Res JT - Cancer research JID - 2984705R SB - IM EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 13:54 PHST- 2026/10/26 00:00 [accepted] PHST- 2026/02/03 00:00 [received] PHST- 2026/08/18 00:00 [revised] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:54 [entrez] AID - 789686 [pii] AID - 10.1158/0008-5472.CAN-26-0563 [doi] PST - aheadofprint SO - Cancer Res. 2026 Oct 27. doi: 10.1158/0008-5472.CAN-26-0563. PMID- 42945289 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Sound and music in video games: A systematic review of player engagement and a meta-analysis of positively valenced player experience. PG - e0361519 LID - 10.1371/journal.pone.0361519 [doi] LID - e0361519 AB - Video game sound and music have previously been found to influence player engagement and affective responses during gameplay. However, a systematic investigation into the full extent of these effects has not been conducted. This review examined the influence of music and sound on players' affective responses and engagement during video game play, with a meta-analysis of positively valenced experience (enjoyment, fun or positive affect). Fourteen studies met the inclusion criteria, six of which provided data for the meta-analysis. Game audio was associated with more positively valenced experience than silence (g = 0.48, 95% CI [0.22, 0.74]; small-sample adjusted 95% CI [0.14, 0.82]). Heterogeneity (I(2) = 58%) was accounted for by one self-selected music condition; without it, the effect was smaller and homogeneous (g = 0.34, 95% CI [0.18, 0.50]; I(2) = 0%). In exploratory subgroup analyses, sound effects showed a consistent benefit (g = 0.46), whereas the added effect of music over game sound was uncertain (g = 0.31, adjusted 95% CI [-0.07, 0.68]). In the narrative synthesis, music and sound were often associated with higher immersion, flow and motivation. Thirteen of the 14 studies were rated at low risk of bias. Evidence that game audio improves players' affective experience is promising but preliminary; its effect on engagement has not yet been tested quantitatively, and further research and standardisation of methods are needed. CI - Copyright: (c) 2026 Heikkila et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Heikkila, Enja AU - Heikkila E AUID- ORCID: 0009-0009-6703-9931 AD - Department of Music, Art and Culture Studies, University of Jyvaskyla, Jyvaskyla, Finland. FAU - Danso, Andrew AU - Danso A AUID- ORCID: 0009-0008-6461-7831 AD - Department of Music, Art and Culture Studies, University of Jyvaskyla, Jyvaskyla, Finland. AD - Centre of Excellence in Music, Mind, Body and Brain, University of Jyvaskyla, Jyvaskyla, Finland. FAU - Luck, Geoff AU - Luck G AD - Department of Music, Art and Culture Studies, University of Jyvaskyla, Jyvaskyla, Finland. AD - Centre of Excellence in Music, Mind, Body and Brain, University of Jyvaskyla, Jyvaskyla, Finland. LA - eng PT - Journal Article PT - Meta-Analysis PT - Systematic Review DEP - 20261027 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - *Music/psychology MH - *Video Games/psychology MH - Humans MH - *Sound PMC - PMC13712732 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:54 PHST- 2026/04/08 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:54 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PONE-D-26-15493 [pii] AID - 10.1371/journal.pone.0361519 [doi] PST - epublish SO - PLoS One. 2026 Oct 27;21(10):e0361519. doi: 10.1371/journal.pone.0361519. eCollection 2026. PMID- 42945288 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 2767-3375 (Electronic) IS - 2767-3375 (Linking) VI - 6 IP - 10 DP - 2026 TI - Etiology and incidence of diarrhea requiring hospitalization in children under 5 years of age in 31 low- and middle-income countries: Findings from the Global Pediatric Diarrhea Surveillance network, 2017-2022. PG - e0006655 LID - 10.1371/journal.pgph.0006655 [doi] LID - e0006655 AB - Diarrhea remains a leading cause of child morbidity and mortality. Improved and ongoing estimates of the etiology of hospitalized pediatric diarrhea in low- and middle-income countries (LMICs) are needed to help prioritize and evaluate the use of existing and upcoming vaccines and interventions. The Global Pediatric Diarrhea Surveillance (GPDS) network is a World Health Organization (WHO)-coordinated public health surveillance network investigating the etiology of hospitalized diarrhea among children aged <5 years in LMICs. The GPDS network enrolls children hospitalized with diarrhea at 38 sentinel surveillance sites in 31 LMICs. Randomly selected stool specimens were tested by TaqMan Array Card quantitative reverse-transcription polymerase chain reaction (qPCR) for 16 pathogens associated with diarrhea. We estimated pathogen-specific attributable fractions (AFs) and incidence of diarrheal hospitalizations at the global, regional, and country levels during 3 time periods: 2017-2018, 2019-2020, and 2021-2022, with a focus on the most recent results. During 2017-2022, the GPDS network enrolled 70,750 children aged <5 years hospitalized with diarrhea, of which 16,458 (23.3%) were randomly selected for qPCR testing. The most prevalent pathogen detected, regardless of quantity or modeled etiologic attribution, was rotavirus (weighted prevalence 30.4%), followed by adenovirus 40/41 (19.1%), norovirus (17.9%), Shigella (14.1%), and Campylobacter jejuni/coli (8.6%). Overall, in 2017-2022, rotavirus was the leading etiology globally (AF 32.5%; 95% Confidence Interval (CI): 27.4, 37.6), followed by Shigella (9.8%; 8.4, 11.3), adenovirus 40/41 (8.6%; 6.3, 10.8) and norovirus (6.7%; 5.6, 7.7). Over time, rotavirus consistently declined from an AF of 36.7% (95% CI: 28.7, 46.7) in 2017-2018 to 26.7% (20.7, 34.1) in 2021-2022. Norovirus AF increased slightly from 6.2% (4.7, 7.7) in 2017-2018 to 7.3% (5.0, 9.2) in 2021-2022. Global Shigella burden remained stable, and adenovirus 40/41 demonstrated significant volatility, peaking globally in 2019-2020 (11.9%; 5.9, 17.5). In 2021-2022, rotavirus was the leading cause of hospitalized diarrhea in 6 of 9 geographic groupings, norovirus predominated in Central and South America, and Shigella was the leading etiology in South Asia. In the subset of countries that had introduced rotavirus vaccine, the leading etiologies in 2021-2022 were rotavirus (18.4%; 15.8, 21.5) and Shigella (16.4%; 11.8, 21.1). In 2021-2022, rotavirus had the highest attributable incidence of hospitalized diarrhea in children (2.3 per 1,000 child-years; 1.8, 3.0), followed by Shigella (0.9; 0.7, 1.1), norovirus (0.6; 0.4, 0.8) and adenovirus 40/41 (0.6; 0.4, 0.8). Despite the widespread use of rotavirus vaccines, rotavirus remained the leading cause of severe diarrhea among children aged <5 years in LMICs globally. However, the proportion of pediatric diarrhea attributable to rotavirus consistently declined from 2017-2018-2021-2022, and there were notable differences in the distribution of diarrheal etiologies between regions and across time periods. Shigella, norovirus, and enteric adenoviruses were also associated with a substantial burden of disease. Improving the efficacy and coverage of rotavirus vaccination and prioritizing interventions against other enteric pathogens could further reduce diarrhea morbidity and mortality. CI - Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication. FAU - Soeters, Heidi M AU - Soeters HM AUID- ORCID: 0000-0002-9415-9340 AD - World Health Organization, Geneva, Switzerland. FAU - Antoni, Sebastien AU - Antoni S AUID- ORCID: 0000-0002-0911-0711 AD - World Health Organization, Geneva, Switzerland. FAU - Iyer, Shilpa S AU - Iyer SS AD - World Health Organization, Geneva, Switzerland. FAU - Weldegebriel, Goitom AU - Weldegebriel G AD - World Health Organization Regional Office for Africa, Brazzaville, Congo. FAU - Biey, Joseph AU - Biey J AD - World Health Organization Regional Office for Africa, Brazzaville, Congo. FAU - Mwenda, Jason M AU - Mwenda JM AD - World Health Organization Regional Office for Africa, Brazzaville, Congo. FAU - Rey-Benito, Gloria AU - Rey-Benito G AD - Pan American Health Organization, World Health Organization Regional Office for the Americas, Washington, District of Columbia, United States of America. FAU - Ortiz, Claudia AU - Ortiz C AD - Pan American Health Organization, World Health Organization Regional Office for the Americas, Washington, District of Columbia, United States of America. FAU - Pastore, Roberta AU - Pastore R AUID- ORCID: 0000-0002-4110-774X AD - World Health Organization Regional Office for Europe, Copenhagen, Denmark. FAU - Videbaek, Dovile AU - Videbaek D AUID- ORCID: 0000-0002-5085-0981 AD - World Health Organization Regional Office for Europe, Copenhagen, Denmark. FAU - Singh, Simarjit AU - Singh S AD - World Health Organization Regional Office for Europe, Copenhagen, Denmark. FAU - Njambe, Emmanuel AU - Njambe E AUID- ORCID: 0000-0002-3870-7525 AD - World Health Organization Regional Office for South-East Asia, New Delhi, India. FAU - Sangal, Lucky AU - Sangal L AUID- ORCID: 0000-0002-2553-4768 AD - World Health Organization Regional Office for South-East Asia, New Delhi, India. FAU - Dhongde, Deepak AU - Dhongde D AD - World Health Organization Regional Office for South-East Asia, New Delhi, India. FAU - Grabovac, Varja AU - Grabovac V AD - World Health Organization Regional Office for the Western Pacific, Manila, Philippines. FAU - Logronio, Josephine AU - Logronio J AD - World Health Organization Regional Office for the Western Pacific, Manila, Philippines. FAU - Fahmy, Kamal AU - Fahmy K AD - World Health Organization Regional Office for the Eastern Mediterranean, Cairo, Egypt. FAU - Ghoniem, Amany AU - Ghoniem A AD - World Health Organization Regional Office for the Eastern Mediterranean, Cairo, Egypt. FAU - Armah, George AU - Armah G AD - Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Accra, Ghana. FAU - Dennis, Francis E AU - Dennis FE AD - Noguchi Memorial Institute for Medical Research, College of Health Sciences, University of Ghana, Accra, Ghana. FAU - Seheri, Mapaseka L AU - Seheri ML AD - Diarrhoeal Pathogens Research Unit, Department of Virology, Sefako Makgatho Health Sciences University, Pretoria, South Africa. FAU - Magagula, Nonkululeko AU - Magagula N AD - Diarrhoeal Pathogens Research Unit, Department of Virology, Sefako Makgatho Health Sciences University, Pretoria, South Africa. FAU - Rakau-Nondela, Kebareng AU - Rakau-Nondela K AUID- ORCID: 0000-0003-1094-9669 AD - Diarrhoeal Pathogens Research Unit, Department of Virology, Sefako Makgatho Health Sciences University, Pretoria, South Africa. FAU - Fumian, Tulio M AU - Fumian TM AUID- ORCID: 0000-0001-9654-0172 AD - Instituto Oswaldo Cruz Fiocruz, Rio de Janeiro, Brazil. FAU - Maciel, Irene T A AU - Maciel ITA AUID- ORCID: 0009-0002-3700-3814 AD - Instituto Oswaldo Cruz Fiocruz, Rio de Janeiro, Brazil. FAU - Samoilovich, Elena AU - Samoilovich E AD - Republican Research and Practical Center for Epidemiology and Microbiology, Ministry of Health, Minsk, Belarus. FAU - Semeiko, Galina AU - Semeiko G AUID- ORCID: 0000-0002-2170-3579 AD - Republican Research and Practical Center for Epidemiology and Microbiology, Ministry of Health, Minsk, Belarus. FAU - Varghese, Tintu AU - Varghese T AD - Christian Medical College, Vellore, India. FAU - Thomas, Sarah AU - Thomas S AD - Murdoch Children's Research Institute, Melbourne, Australia. FAU - Bines, Julie E AU - Bines JE AD - Murdoch Children's Research Institute, Melbourne, Australia. FAU - Li, Dandi AU - Li D AD - National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China. FAU - Kabir, Furqan AU - Kabir F AD - Infectious Diseases Research Laboratory, Aga Khan University, Karachi, Pakistan. FAU - Liu, Jie AU - Liu J AD - School of Public Health, Qingdao University, Qingdao, Shandong, China. AD - Division of Infectious Diseases & International Health, University of Virginia, Charlottesville, Virginia, United States of America. FAU - Houpt, Eric R AU - Houpt ER AD - Division of Infectious Diseases & International Health, University of Virginia, Charlottesville, Virginia, United States of America. FAU - Gautam, Rashi AU - Gautam R AD - Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America. FAU - Mirza, Sara A AU - Mirza SA AD - Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America. FAU - Vinje, Jan AU - Vinje J AUID- ORCID: 0000-0002-1530-3675 AD - Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America. FAU - Mulders, Mick N AU - Mulders MN AD - World Health Organization, Geneva, Switzerland. FAU - Tate, Jacqueline E AU - Tate JE AD - Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America. FAU - Parashar, Umesh D AU - Parashar UD AD - Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America. FAU - Platts-Mills, James A AU - Platts-Mills JA AUID- ORCID: 0000-0002-4956-0418 AD - Division of Infectious Diseases & International Health, University of Virginia, Charlottesville, Virginia, United States of America. CN - Global Pediatric Diarrhea Surveillance network LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLOS Glob Public Health JT - PLOS global public health JID - 9918283779606676 PMC - PMC13712721 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:54 PHST- 2026/05/28 00:00 [received] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:54 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PGPH-D-26-02007 [pii] AID - 10.1371/journal.pgph.0006655 [doi] PST - epublish SO - PLOS Glob Public Health. 2026 Oct 27;6(10):e0006655. doi: 10.1371/journal.pgph.0006655. eCollection 2026. PMID- 42945284 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 2042-7158 (Electronic) IS - 0022-3573 (Linking) VI - 78 IP - 10 DP - 2026 Oct 1 TI - Endoplasmic reticulum stress in breast cancer metastasis: mechanisms and therapeutic implications. LID - rgag127 [pii] LID - 10.1093/jpp/rgag127 [doi] AB - OBJECTIVES: To summarize the roles of endoplasmic reticulum stress (ERS) and the unfolded protein response (UPR) in breast cancer (BC) invasion and metastasis and discuss the therapeutic potential of targeting these processes. METHODS: This review examines the molecular mechanisms linking ERS/UPR signaling to BC progression and synthesizes ERS-targeted anti-metastatic strategies reported over the past decade, including pathway inhibition, ERS hyperactivation, induction of immunogenic cell death, and combination approaches. KEY FINDINGS: ERS is an adaptive response to adverse conditions within the tumor microenvironment (TME), including hypoxia and nutrient deprivation. ERS/UPR signaling can reshape tumor cell behavior and the TME to promote invasion and metastasis. Clinically, elevated ERS activity is associated with poor prognosis, shorter survival, and reduced therapeutic responsiveness. Therapeutic strategies include inhibiting pro-survival ERS pathways, hyperactivating ERS to induce tumor cell death, and exploiting ERS to trigger immunogenic cell death. However, the context-dependent and subtype-specific roles of ERS, together with potential on-target toxicity in normal tissues, complicate clinical translation. CONCLUSIONS: ERS represents a therapeutically actionable vulnerability in metastatic BC. Improved patient stratification using ERS/UPR biomarkers, rational combination regimens, and tumor-selective delivery platforms may help maximize therapeutic efficacy while minimizing systemic toxicity. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Royal College of Pharmacy. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Xu, Rujie AU - Xu R AD - College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, P. R. China. FAU - Foo, Wenxin AU - Foo W AD - College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, P. R. China. FAU - Luo, Lihua AU - Luo L AD - College of Pharmaceutical Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou, Zhejiang 310058, P. R. China. FAU - Liu, Xu AU - Liu X AUID- ORCID: 0009-0007-4488-5225 AD - The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, China. AD - Guangdong Clinical Research Academy of Chinese Medicine, Guangdong 510006, China. LA - eng PT - Journal Article PT - Review PL - England TA - J Pharm Pharmacol JT - The Journal of pharmacy and pharmacology JID - 0376363 RN - 0 (Antineoplastic Agents) SB - IM MH - Humans MH - *Breast Neoplasms/pathology/drug therapy/metabolism MH - *Endoplasmic Reticulum Stress/drug effects/physiology MH - Female MH - Unfolded Protein Response/drug effects/physiology MH - Neoplasm Metastasis MH - Animals MH - Tumor Microenvironment MH - Signal Transduction/drug effects MH - *Antineoplastic Agents/pharmacology/therapeutic use OTO - NOTNLM OT - breast cancer OT - cellular mechanism OT - endoplasmic reticulum stress OT - therapeutic targets OT - tumor metastasis EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 13:53 PHST- 2026/06/12 00:00 [received] PHST- 2026/08/24 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:53 [entrez] AID - 9003174 [pii] AID - 10.1093/jpp/rgag127 [doi] PST - ppublish SO - J Pharm Pharmacol. 2026 Oct 1;78(10):rgag127. doi: 10.1093/jpp/rgag127. PMID- 42945283 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 2042-7158 (Electronic) IS - 0022-3573 (Linking) VI - 78 IP - 10 DP - 2026 Oct 1 TI - Bioengineered multifunctional nanomedicine for cancer theranostics: integrating targeted drug delivery, molecular imaging, and precision medicine. LID - rgag131 [pii] LID - 10.1093/jpp/rgag131 [doi] AB - OBJECTIVES: Cancer remains a major global health challenge despite significant advances in diagnosis and treatment. Conventional therapies, including chemotherapy, radiotherapy, and surgery, are frequently constrained by non-specific drug distribution, systemic toxicity, multidrug resistance (MDR), and limitations in early tumor detection. This review aims to critically examine recent advances in nanomedicine for targeted cancer therapy and diagnostic imaging, with particular emphasis on nanocarrier platforms, tumor-targeting strategies, stimuli-responsive drug delivery, and nanoparticle-based imaging. METHODS: A comprehensive review of the recent literature was undertaken to evaluate the application of diverse nanomedicine platforms in oncology. Major nanocarrier systems, including liposomes, polymeric nanoparticles, dendrimers, metallic nanoparticles, and quantum dots, were assessed with respect to their physicochemical properties, drug-loading capacity, controlled-release characteristics, targeting potential, and therapeutic applications. Passive and active targeting approaches, including the enhanced permeability and retention (EPR) effect and ligand-mediated targeting, were examined. The applications of stimuli-responsive nanocarriers triggered by tumor-associated pH, temperature, and enzymatic conditions were also evaluated. In addition, the role of nanomedicine in MRI, CT, PET, and fluorescence imaging was reviewed. KEY FINDINGS: Nanomedicine provides substantial opportunities for improving the precision and efficacy of cancer treatment by enhancing drug stability, bioavailability, tumor accumulation, and controlled drug release while potentially reducing exposure of healthy tissues. Active and passive targeting strategies can improve the selective delivery of therapeutic agents to tumor sites, whereas stimuli-responsive systems offer additional spatial and temporal control over drug release. Nanoparticle-based contrast and imaging agents have demonstrated considerable potential for enhancing imaging sensitivity, facilitating earlier tumor detection, and enabling real-time assessment of therapeutic responses. Importantly, several nanomedicine platforms also show promise in overcoming MDR and improving overall therapeutic efficiency. However, challenges related to nanotoxicity, pharmacokinetics, manufacturing scalability, regulatory requirements, and long-term clinical safety continue to limit widespread clinical translation. CONCLUSIONS: Nanomedicine represents a rapidly advancing and potentially transformative approach to cancer diagnosis and treatment. Integration of targeted drug delivery, stimuli-responsive nanocarriers, advanced imaging, and theranostic strategies may contribute to more precise and personalized oncology. Continued investigation into safety, manufacturing, regulatory standardization, and clinical validation will be essential for translating promising nanomedicine platforms from experimental studies into routine cancer care. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of the Royal College of Pharmacy. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Dubey, Shivangi AU - Dubey S AD - Department of Mechanical Engineering, Rajiv Gandhi Institute of Petroleum Technology, Amethi 229304, Uttar Pradesh, India. FAU - Prakash, Sneha AU - Prakash S AD - University Centre for Research & Development, Chandigarh University, Mohali 140413, Punjab, India. FAU - Deshwal, Ravi K AU - Deshwal RK AD - Institute of Bioscience and Technology, Shri Ramswaroop Memorial University, Lucknow, Deva Road, Barabanki 225003, Uttar Pradesh, India. FAU - Dwivedi, Manish AU - Dwivedi M AUID- ORCID: 0000-0001-5217-9039 AD - Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow Campus, Lucknow 226028, Uttar Pradesh, India. AD - Research Cell, Amity University Uttar Pradesh, Lucknow Campus, Lucknow 226028, Uttar Pradesh, India. LA - eng PT - Journal Article PT - Review PL - England TA - J Pharm Pharmacol JT - The Journal of pharmacy and pharmacology JID - 0376363 RN - 0 (Antineoplastic Agents) RN - 0 (Drug Carriers) SB - IM MH - Humans MH - *Neoplasms/drug therapy/diagnosis/diagnostic imaging MH - *Theranostic Nanomedicine/methods MH - *Precision Medicine/methods MH - Animals MH - *Drug Delivery Systems/methods MH - *Molecular Imaging/methods MH - Nanoparticles MH - Nanomedicine/methods MH - *Antineoplastic Agents/administration & dosage MH - Drug Carriers/chemistry OTO - NOTNLM OT - cancer imaging OT - drug delivery systems OT - nanomedicine OT - nanoparticles OT - targeted cancer therapy EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 13:53 PHST- 2026/07/20 00:00 [received] PHST- 2026/09/03 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:53 [entrez] AID - 9003172 [pii] AID - 10.1093/jpp/rgag131 [doi] PST - ppublish SO - J Pharm Pharmacol. 2026 Oct 1;78(10):rgag131. doi: 10.1093/jpp/rgag131. PMID- 42945281 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - High sensitivity infrared gas sensing and filtering using metasurfaces plasmonic ring resonators. PG - e0353973 LID - 10.1371/journal.pone.0353973 [doi] LID - e0353973 AB - This work investigates a plasmonic metal-insulator-metal (MIM) ring structure periodically coupled to dual resonators and a defect cell, engineered for infrared filtering and gas sensing. The optical response is modeled numerically using the finite element method in COMSOL Multiphysics, enabling accurate analysis of strong field confinement and loss in the subwavelength MIM geometry. By tuning the geometrical parameters of the rectangular and lateral resonators and of the defect cavity, we show that the transmission spectrum, band-gap position, and defect resonance wavelength can be precisely controlled, and that introducing a defect resonator into a periodic dual-resonator array generates a sharp, highly localized mode inside the plasmonic band gap. Small variations of the refractive index in the gas-filled device lead to clear, nearly linear shifts of this defect resonance, yielding a high sensitivity of about 1920 nm/RIU together with a Q-factor above 100 and a low detection limit, so that sensitivity, spectral selectivity, and detection capability are simultaneously enhanced. These results highlight the novelty of combining periodic band-gap engineering, dual-resonator coupling, and defect optimization in a single MIM platform, and demonstrate that the proposed structure can function as both a narrowband photonic filter and a high-performance infrared gas sensor in a compact, integrated configuration. CI - Copyright: (c) 2026 Mimoun et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Mimoun, El-Aouni AU - Mimoun EA AD - Laboratory of Materials, Waves, Energy and Environment, Team of Waves, Acoustics, Photonics and Materials, Mohamed I University, Oujda, Morocco. FAU - Hennache, Ali AU - Hennache A AD - Deanship of Scientific Research, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh, Saudi Arabia. FAU - Zaky, Mahmoud A AU - Zaky MA AD - Department of Mathematics and Statistics, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh, Saudi Arabia. FAU - Youssef, Ben-Ali AU - Youssef BA AD - Engineering Sciences Laboratory (LSI), Multidisciplinary Faculty of Taza, Sidi Mohamed Ben Abdellah University, Taza Gare, Morocco. FAU - Abdelouahed, Essahlaoui AU - Abdelouahed E AD - Engineering Sciences Laboratory (LSI), Multidisciplinary Faculty of Taza, Sidi Mohamed Ben Abdellah University, Taza Gare, Morocco. FAU - Driss, Bria AU - Driss B AD - Laboratory of Materials, Waves, Energy and Environment, Team of Waves, Acoustics, Photonics and Materials, Mohamed I University, Oujda, Morocco. FAU - Alzahrani, Ali AU - Alzahrani A AD - Department of Physics, Al-Qunfudah University College, Umm Al-Qura University, Makkah, Saudi Arabia. FAU - Zaky, Zaky A AU - Zaky ZA AUID- ORCID: 0000-0002-8300-7755 AD - Physics Department, Faculty of Science, Beni-Suef University, Beni Suef, Egypt. AD - Department of Mathematics and Natural Sciences, Gulf University for Science and Technology, Mishref, Kuwait. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (Gases) RN - 0 (Metals) SB - IM MH - *Gases/analysis MH - *Infrared Rays MH - *Surface Plasmon Resonance/instrumentation/methods MH - Equipment Design MH - Metals/chemistry PMC - PMC13712699 COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:52 PHST- 2026/04/02 00:00 [received] PHST- 2026/07/01 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:52 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PONE-D-26-16358 [pii] AID - 10.1371/journal.pone.0353973 [doi] PST - epublish SO - PLoS One. 2026 Oct 27;21(10):e0353973. doi: 10.1371/journal.pone.0353973. eCollection 2026. PMID- 42945279 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Airway management and anaesthetic interventions for children with cleft lip and/or palate undergoing general anaesthesia: Protocol for a systematic review and meta-analysis. PG - e0361327 LID - 10.1371/journal.pone.0361327 [doi] LID - e0361327 AB - BACKGROUND: Children with cleft lip and/or palate may present unique challenges during perioperative airway management because of altered upper-airway and craniofacial anatomy. These children frequently undergo surgical procedures requiring general anaesthesia and airway instrumentation. Several airway-management and anaesthetic techniques have been evaluated to facilitate tracheal intubation and reduce perioperative airway complications, but the comparative evidence from randomised controlled trials has not been systematically evaluated. METHODS: This systematic review and meta-analysis will include randomised controlled trials involving children < 18 years with cleft lip and/or palate undergoing general anaesthesia. Eligible studies will compare alternative techniques or devices for airway management or other anaesthetic strategies between two groups of cleft lip/palate patients. The primary outcome will be difficulty in tracheal intubation. Where definitions vary substantially, outcomes will be analysed separately or synthesised narratively. Secondary outcomes will include difficult mask ventilation, oxygen desaturation, airway obstruction, difficult laryngoscopy, first-attempt intubation success, number of intubation attempts, time to successful intubation or airway establishment, failed intubation and other perioperative airway or respiratory adverse events. Two reviewers will independently select RCTs, extract data and assess risk of bias. Risk of bias will be evaluated using the Cochrane Risk of Bias 2 tool. Random-effects meta-analysis will be performed where clinically appropriate. Risk ratios will be used for dichotomous outcomes and mean differences or standardized mean differences for continuous outcomes. Certainty of evidence for important outcomes will be assessed using the Grading of Recommendations Assessment, Development and Evaluation (GRADE) approach. REGISTRATION AND REVIEW STATUS: The review was prospectively registered in PROSPERO on 10 March 2026 (CRD420261326076). Full-text screening and data extraction are ongoing and not yet complete. Protocol amendments are documented transparently in PROSPERO. CI - Copyright: (c) 2026 Sumi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Sumi, Sharmin Akter AU - Sumi SA AD - Department of Anatomy, Bangladesh Medical University, Dhaka, Bangladesh. FAU - Arin, Kazi Mahzabin AU - Arin KM AUID- ORCID: 0009-0004-0064-3847 AD - Department of Anaesthesia, Analgesia and Intensive Care Medicine, Bangladesh Medical University, Dhaka, Bangladesh. FAU - Shams, Shahriar Mohd AU - Shams SM AUID- ORCID: 0000-0002-1622-4661 AD - Department of Orthodontics, Bangladesh Medical University, Dhaka, Bangladesh. FAU - Rafique, Tanzila AU - Rafique T AUID- ORCID: 0000-0002-6617-907X AD - Department of Orthodontics, Bangladesh Medical University, Dhaka, Bangladesh. FAU - Orchi, Keertika AU - Orchi K AUID- ORCID: 0009-0009-6173-6509 AD - Department of Public Health and Informatics, Bangladesh Medical University, Dhaka, Bangladesh. FAU - Chowdhury, Arna AU - Chowdhury A AUID- ORCID: 0000-0001-5468-3431 AD - Department of Public Health and Informatics, Bangladesh Medical University, Dhaka, Bangladesh. FAU - Rahman, Mohammad Meshbahur AU - Rahman MM AD - Department of Biostatistics, National Institute of Preventive and Social Medicine, Dhaka, Bangladesh. FAU - Haque, Md Atiqul AU - Haque MA AD - Department of Public Health and Informatics, Bangladesh Medical University, Dhaka, Bangladesh. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Humans MH - *Cleft Lip/surgery MH - *Anesthesia, General/methods MH - *Cleft Palate/surgery MH - Systematic Reviews as Topic MH - Meta-Analysis as Topic MH - *Airway Management/methods MH - Child MH - Intubation, Intratracheal/methods MH - Randomized Controlled Trials as Topic MH - Pediatric Anesthesia PMC - PMC13712514 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:44 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:44 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PONE-D-26-43191 [pii] AID - 10.1371/journal.pone.0361327 [doi] PST - epublish SO - PLoS One. 2026 Oct 27;21(10):e0361327. doi: 10.1371/journal.pone.0361327. eCollection 2026. PMID- 42945273 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Multimodal lifestyle intervention during neoadjuvant chemotherapy in patients with early breast cancer as a strategy to improve treatment response: Protocol of the DIANA randomized clinical trial. PG - e0361188 LID - 10.1371/journal.pone.0361188 [doi] LID - e0361188 AB - Breast cancer (BC) represents a major global health concern. Lifestyle factors influence both prevention and prognosis, but evidence on their impact on oncological response to BC treatment remains limited. We present the protocol of a randomized clinical trial designed to assess whether a multimodal intervention-combining physical exercise, psychological support, and nutritional counseling during neoadjuvant chemotherapy (NAC)-could improve pathological response, measured by the Residual Cancer Burden (RCB) index, in women with early BC. A total of 216 women will be randomized (1:1) to control (standard of care, n = 108) or intervention (n = 108). The intervention consists of a supervised, individualized multimodal prehabilitation program that combines physical exercise, psychotherapy, and nutritional support delivered throughout NAC (4-6 months). Tumor pathology, including microenvironment analysis, will be assessed before NAC and after surgery. Data will be collected at baseline, preoperatively, and at 1 and 12 months postoperatively. Long-term oncological outcomes will be reported annually for a minimum of 5 years, with the intention to extend to 10 years. We hypothesize that the intervention group will show higher pathological response rates than controls, with a targeted 20-percentage point improvement in the rate of favorable RCB response (fRCB, defined as RCB class 0 or I), from approximately 40% in the control group to 60% in the intervention group. Additional expected benefits include improved tolerance to NAC, better quality of life, enhanced postoperative recovery, and optimized physical, psychological, and nutritional status. Differences in tumor microenvironment profiles are also anticipated. Ethics approval was granted by the Ethics Committee of the Hospital Clinic de Barcelona (HCB/2023/1036, 26 March 2024). Written informed consent will be obtained from all participants. The DIANA trial will provide evidence on multimodal prehabilitation as a non-invasive, cost-efficient strategy to improve both health and oncological outcomes in BC. Findings will be disseminated via peer-reviewed publications, conference presentations, and patient groups. Trial Registration Number: NCT07107594. CI - Copyright: (c) 2026 Castillo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Castillo, Helena AU - Castillo H AUID- ORCID: 0009-0008-2741-2765 AD - Department of Gynecology, Hospital Clinic i Provincial de Barcelona (HCPB), Barcelona, Spain. FAU - Sebio-Garcia, Raquel AU - Sebio-Garcia R AUID- ORCID: 0000-0002-7167-4737 AD - Fundacio Clinic per a la Recerca Biomedica (FCRB) - IDIBAPS, Barcelona, Spain. AD - Department of Physiotherapy and Rehabilitation, Hospital Clinic i Provincial de Barcelona (HCPB), Barcelona, Spain. FAU - Martinez-Palli, Graciela AU - Martinez-Palli G AUID- ORCID: 0000-0002-7163-8090 AD - Department of Anesthesiology, Hospital Clinic i Provincial de Barcelona (HCPB), Barcelona, Spain. FAU - Siso, Marina AU - Siso M AD - Department of Nutrition and Dietetics, Hospital Clinic i Provincial de Barcelona (HCPB), Barcelona, Spain. FAU - Garriz, Miguel AU - Garriz M AUID- ORCID: 0000-0002-0467-9490 AD - Department of Psychology, Hospital Clinic i Provincial de Barcelona (HCPB), Barcelona, Spain. FAU - Sanfeliu, Esther AU - Sanfeliu E AD - Department of Pathology, Hospital Clinic i Provincial de Barcelona (HCPB), Barcelona, Spain. FAU - Braso, Fara AU - Braso F AD - Department of Biology, Hospital Clinic i Provincial de Barcelona (HCPB), Barcelona, Spain. FAU - Cebrecos, Isaac AU - Cebrecos I AD - Department of Gynecology, Hospital Clinic i Provincial de Barcelona (HCPB), Barcelona, Spain. FAU - Torras, Ines AU - Torras I AD - Department of Gynecology, Hospital Clinic i Provincial de Barcelona (HCPB), Barcelona, Spain. FAU - Quintas, Lara AU - Quintas L AD - Department of Gynecology, Hospital Clinic i Provincial de Barcelona (HCPB), Barcelona, Spain. FAU - Gomez, Raquel AU - Gomez R AD - Department of Medical Oncology, Hospital Clinic i Provincial de Barcelona (HCPB), Barcelona, Spain. FAU - Ganau, Sergi AU - Ganau S AD - Department of Radiology, Hospital Clinic i Provincial de Barcelona (HCPB), Barcelona, Spain. FAU - Diaz-Feijoo, Berta AU - Diaz-Feijoo B AD - Department of Gynecology, Hospital Clinic i Provincial de Barcelona (HCPB), Barcelona, Spain. AD - Fundacio Clinic per a la Recerca Biomedica (FCRB) - IDIBAPS, Barcelona, Spain. FAU - Mension, Eduard AU - Mension E AD - Department of Gynecology, Hospital Clinic i Provincial de Barcelona (HCPB), Barcelona, Spain. AD - Fundacio Clinic per a la Recerca Biomedica (FCRB) - IDIBAPS, Barcelona, Spain. LA - eng SI - ClinicalTrials.gov/NCT07107594 PT - Clinical Trial Protocol PT - Journal Article DEP - 20261027 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Humans MH - Female MH - *Breast Neoplasms/therapy/drug therapy/pathology MH - *Neoadjuvant Therapy/methods MH - *Life Style MH - Randomized Controlled Trials as Topic MH - Treatment Outcome MH - Adult MH - Combined Modality Therapy MH - Quality of Life MH - Middle Aged MH - Chemotherapy, Adjuvant PMC - PMC13712585 COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:44 PHST- 2026/03/21 00:00 [received] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:44 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PONE-D-26-09274 [pii] AID - 10.1371/journal.pone.0361188 [doi] PST - epublish SO - PLoS One. 2026 Oct 27;21(10):e0361188. doi: 10.1371/journal.pone.0361188. eCollection 2026. PMID- 42945270 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 2767-3375 (Electronic) IS - 2767-3375 (Linking) VI - 6 IP - 10 DP - 2026 TI - Facial cleanliness, environmental factors, and the burden of trachoma in a pastoral conflict area of Tiaty East, Baringo County, Kenya. PG - e0007534 LID - 10.1371/journal.pgph.0007534 [doi] LID - e0007534 AB - Trachoma remains the leading infectious cause of preventable blindness globally, disproportionately affecting marginalized and impoverished communities. Despite global and national efforts guided by the World Health Organization's (WHO) SAFE strategy (Surgery, Antibiotics, Facial cleanliness, Environmental improvement), the latter two components remain under-implemented. In Kenya, trachoma remains endemic in 13 of 47 counties, including Baringo. This cross-sectional study was conducted in Tiaty East Sub-County, a trachoma Sub-Evaluation Unit in Baringo, to determine the burden of trachoma and assess facial cleanliness and environmental improvement for control of the disease at the household level. Using a structured questionnaire and observation checklist, data were collected from 178 randomly selected households. A total of 279 children aged 1-9 years were examined for trachomatous inflammation-follicular (TF). The overall TF prevalence was 5.02% (95% CI: 2.8-8.3), with the confidence interval overlapping with the WHO elimination threshold of <5%. Facial cleanliness was sub-optimal, with 91.8% of children having visibly unclean faces. Latrine coverage was low (21.3%), and 99.1% of households without latrines practiced open defecation. Most households (61.8%) sourced water from a dam, and 80.9% required more than 30 minutes to fetch it. Handwashing stations were absent in 94% of households. Final multivariable analysis identified older caregiver age (> 20 years) as significantly associated with lower odds of TF (21-30 years: AOR = 0.089, 95% CI: 0.018-0.447; 31-40 years: AOR = 0.259, 95% CI: 0.068-0.991; >/= 40 years: AOR = 0.160, 95% CI: 0.029-0.871). Caregiver awareness of poor hygiene as a trachoma-related factor was associated with higher odds of TF (AOR = 3.795; 95% CI:1.272-11.324), suggesting a critical knowledge-practice gap. Although TF prevalence was slightly above the threshold based on a few events, with poor F&E coverage, there remain sub-EU-level gaps in trachoma control. In this context, strengthening the F&E aspects of the SAFE strategy with focused investments on WASH and behavior-based hygiene promotion will be important to the achievement of sub-EU disease elimination. CI - Copyright: (c) 2026 Njogu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Njogu, Loise Wanjira AU - Njogu LW AUID- ORCID: 0009-0000-6247-201X AD - School of Public Health, Department of Environmental Health and Disease Control, Jomo Kenyatta Institute of Agriculture and Technology, Nairobi, Kenya. AD - Kenya Medical Research Institute (KEMRI), Graduate School, Nairobi, Kenya. FAU - Makalliwa, George A AU - Makalliwa GA AUID- ORCID: 0000-0002-3167-9525 AD - School of Public Health, Department of Environmental Health and Disease Control, Jomo Kenyatta Institute of Agriculture and Technology, Nairobi, Kenya. FAU - Kimani, Bridget AU - Kimani B AD - Kenya Medical Research Institute (KEMRI), Eastern & Southern Africa Centre of International Parasitic Control (ESACIPAC), Nairobi, Kenya. FAU - Karimi, Kellen AU - Karimi K AD - Department of Public and Global Health, Faculty of Health Sciences, University of Nairobi, Nairobi, Kenya. FAU - Kibet, Zephania AU - Kibet Z AD - Department of Health, Baringo County, Kenya, Government of Baringo. FAU - Njomo, Doris AU - Njomo D AUID- ORCID: 0000-0003-3935-4036 AD - Kenya Medical Research Institute (KEMRI), Eastern & Southern Africa Centre of International Parasitic Control (ESACIPAC), Nairobi, Kenya. FAU - Watitu, Titus AU - Watitu T AD - Kenya National Public Health Institute (KNPHI), Ministry of Health, Nairobi, Kenya. FAU - Gichuki, Paul M AU - Gichuki PM AUID- ORCID: 0000-0001-6558-5538 AD - Kenya Medical Research Institute (KEMRI), Eastern & Southern Africa Centre of International Parasitic Control (ESACIPAC), Nairobi, Kenya. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLOS Glob Public Health JT - PLOS global public health JID - 9918283779606676 PMC - PMC13712619 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:44 PHST- 2025/10/11 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:44 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PGPH-D-25-03058 [pii] AID - 10.1371/journal.pgph.0007534 [doi] PST - epublish SO - PLOS Glob Public Health. 2026 Oct 27;6(10):e0007534. doi: 10.1371/journal.pgph.0007534. eCollection 2026. PMID- 42945269 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Mutation-resolved ABL1 inhibitor resistance prediction under mutation-disjoint and temporal cross-study validation. PG - e0353101 LID - 10.1371/journal.pone.0353101 [doi] LID - e0353101 AB - Computational prediction of ABL1 tyrosine-kinase inhibitor (TKI) resistance is difficult because available mutation-drug datasets are small, imbalanced, and heterogeneous. This study evaluates structure-aware resistance prediction under mutation-disjoint internal validation and temporal cross-study outcome validation. The development benchmark comprised 144 ABL1 mutation-TKI records (19 resistant and 125 susceptible) labelled by a ten-fold affinity-loss criterion. A fixed panel of 11 documented structural and energetic predictors was used to minimize data-adaptive feature selection within the validation framework. A calibrated ensemble combining Elastic-Net logistic regression, radial-basis-function support-vector machines, random forests, and XGBoost was evaluated using mutation-disjoint five-fold grouped out-of-fold prediction repeated ten times. Temporal validation used a later prime-editing resistance map, yielding 84 exact mutation-TKI matches across imatinib, nilotinib, bosutinib, and ponatinib; 61 had binary Sensitive/Resistant outcomes and 23 were retained as Intermediate. The 11-feature stack achieved grouped internal AUROC 0.645 (95% CI 0.429-0.830) and PR-AUC 0.230, with mean +/- SD AUROC 0.583 +/- 0.101 across repeated grouped splits. On the 61 binary later-study outcomes, AUROC was 0.605 (95% CI 0.431-0.874) and PR-AUC 0.835, modestly above the resistance-prevalence baseline of 0.770. External calibration was poor (Brier score 0.583; calibration slope 0.441). Elastic-Net showed somewhat higher temporal discrimination (AUROC 0.643; PR-AUC 0.877). These results indicate limited transportability across mutation identities and experimental platforms and support use of the framework for hypothesis-generating resistance screening rather than clinically calibrated treatment selection. CI - Copyright: (c) 2026 Deif et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Deif, Mohanad A AU - Deif MA AUID- ORCID: 0000-0002-4388-1480 AD - Research Institute of Sciences and Engineering, University of Sharjah, Sharjah, United Arab Emirates. AD - Department of Computer Science, College of Information Technology, Misr University for Science and Technology (MUST), Giza, Egypt. FAU - Hafez, Mohamed A AU - Hafez MA AD - Faculty of Engineering, INTI International University, Nilai, Malaysia. AD - Shinawatra University, Bangkok, Pathum Thani, Thailand. FAU - Khishe, Mohammad AU - Khishe M AD - Applied Science Research Center, Applied Science Private University, Amman, Jordan. FAU - Solyman, Ahmed AU - Solyman A AUID- ORCID: 0000-0002-2881-8635 AD - Department of Engineering, School of Science and Engineering, Glasgow Caledonian University, Glasgow, United Kingdom. FAU - Elhaty, Ismail A M AU - Elhaty IAM AD - Istanbul Gelisim University, Istanbul, Turkey. LA - eng PT - Journal Article PT - Validation Study DEP - 20261027 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (Protein Kinase Inhibitors) RN - EC 2.7.10.2 (Proto-Oncogene Proteins c-abl) RN - EC 2.7.10.2 (ABL1 protein, human) RN - F41401512X (nilotinib) RN - 0 (Pyrimidines) RN - 8A1O1M485B (Imatinib Mesylate) RN - 0 (Nitriles) RN - 0 (Pyridazines) RN - 5018V4AEZ0 (bosutinib) RN - 0 (Quinolines) RN - 4340891KFS (ponatinib) RN - 0 (Aniline Compounds) RN - 0 (Imidazoles) SB - IM MH - Humans MH - *Mutation MH - *Protein Kinase Inhibitors/pharmacology/therapeutic use/chemistry MH - *Proto-Oncogene Proteins c-abl/genetics/antagonists & inhibitors MH - *Drug Resistance, Neoplasm/genetics MH - Pyrimidines/pharmacology MH - Imatinib Mesylate/pharmacology MH - Nitriles/pharmacology MH - Pyridazines/pharmacology MH - Quinolines/pharmacology MH - Aniline Compounds/pharmacology MH - Imidazoles/pharmacology MH - Boosting Machine Learning Algorithms MH - Random Forest PMC - PMC13712595 COIS- The Competing Interests section states that M.A.H. is employed by INTI International University and that this does not alter adherence to PLOS ONE policies on sharing data and materials. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:44 PHST- 2026/06/17 00:00 [received] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:44 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PONE-D-26-30416 [pii] AID - 10.1371/journal.pone.0353101 [doi] PST - epublish SO - PLoS One. 2026 Oct 27;21(10):e0353101. doi: 10.1371/journal.pone.0353101. eCollection 2026. PMID- 42945267 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Environmental modulation of methyl farnesoate release in two clones of Daphnia pulex differing in the sensitivity to exogenous stimuli. PG - e0361189 LID - 10.1371/journal.pone.0361189 [doi] LID - e0361189 AB - Daphnia is a widely recognised model organism in ecotoxicological studies, exhibiting environmental sex determination. Environmental cues such as temperature, photoperiod and crowding are known to trigger transitions from parthenogenetic to sexual reproduction. Experimental studies based on exogenous exposure suggest that methyl farnesoate (MF), a juvenile hormone-like compound, is associated with male production and predator-induced defensive traits in Daphnia; however, direct evidence of endogenous MF production and environmental dynamics remains limited. In this study, we investigated the release of endogenous MF into the rearing water of Daphnia pulex. Two D. pulex clonal lineages differing in propensity for male, ephippia and neckteeth production were selected. Experimental conditions simulating crowding and different habitat suitability, including variations in population density, food availability, photoperiod and temperature, were investigated. MF was quantified at ultra-trace levels using solid-phase microextraction coupled to gas chromatography-tandem mass spectrometry. In the first generation, MF concentrations decreased from approximately 18.5-20.0 ng/L in controls to 11.0-14.5 ng/L under crowding conditions and 6.7-7.2 ng/L under crowding combined with MK-801. In the second generation, concentrations were approximately 10.5 ng/L in control offspring and 12.0-12.3 ng/L after recovery from crowding. Exposure to crowding over two generations resulted in clone-specific concentrations, whereas MF remained below the limit of quantification of 0.08 ng/L, when parental females had been exposed to MK-801. In the density and seasonal experiments, MF released in the medium was higher release per female under early-spring conditions and in clone S. These variations occurred under conditions promoting male production, ephippia formation and antipredator responses. While not demonstrating a causal endocrine role of MF, our findings provide quantitative and environmentally relevant evidence that D. pulex releases MF into the aquatic environment and this release is modulated by both environmental conditions and genetic background. CI - Copyright: (c) 2026 Bellin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Bellin, Nicolo' AU - Bellin N AD - Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy. AD - Department of Biology, University of Puerto Rico, San Juan, Puerto Rico. FAU - Riboni, Nicolo' AU - Riboni N AD - Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy. FAU - Buschini, Annamaria AU - Buschini A AUID- ORCID: 0000-0002-9877-2767 AD - Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy. FAU - Bianchi, Federica AU - Bianchi F AUID- ORCID: 0000-0001-7880-5624 AD - Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy. FAU - Careri, Maria AU - Careri M AUID- ORCID: 0000-0002-4558-5001 AD - Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy. FAU - Conciatori, Chiara AU - Conciatori C AD - Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy. FAU - Facchini, Clarissa AU - Facchini C AD - Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy. FAU - Filonzi, Laura AU - Filonzi L AUID- ORCID: 0000-0001-8047-959X AD - Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy. FAU - Nicolosi, Stefano AU - Nicolosi S AD - Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy. FAU - Rossi, Valeria AU - Rossi V AD - Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parma, Italy. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 10485-70-8 (methyl farnesoate) RN - 0 (Fatty Acids, Unsaturated) SB - IM MH - Animals MH - *Daphnia pulex/metabolism/drug effects/physiology MH - *Fatty Acids, Unsaturated/metabolism/pharmacology MH - Male MH - *Environment MH - Female MH - Temperature MH - Photoperiod MH - *Daphnia PMC - PMC13712590 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:44 PHST- 2026/08/03 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:44 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PONE-D-26-38849 [pii] AID - 10.1371/journal.pone.0361189 [doi] PST - epublish SO - PLoS One. 2026 Oct 27;21(10):e0361189. doi: 10.1371/journal.pone.0361189. eCollection 2026. PMID- 42945260 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1091-6490 (Electronic) IS - 0027-8424 (Linking) VI - 123 IP - 44 DP - 2026 Nov 3 TI - An innervated and vascularized HNSCC-on-a-chip model built on defined and tunable engineered extracellular matrices. PG - e2602541123 LID - 10.1073/pnas.2602541123 [doi] AB - Understanding the tumor microenvironment requires experimental platforms that faithfully recapitulate its key components. Here, we present an innervated and vascularized head and neck squamous cell carcinoma (HNSCC)-on-a-chip platform built with fully defined and tunable engineered extracellular matrices (eECMs). In a stepwise increase of complexity, we first cocultured patient-derived HNSCC cells, cancer-associated fibroblasts, and endothelial cells within tailored eECMs, revealing extracellular matrix-dependent differences in transcriptional state, multicellular self-organization, and chemotherapeutic sensitivity. We then integrated these three-dimensional constructs into a cancer-vasculature interface, which enabled analysis of eECM-dependent directional collective migration and invasion. Finally, we incorporated trigeminal innervation through injectable 3D human bioengineered trigeminal ganglia, establishing a chip-based innervation-tumor-vasculature tri-interface. Together, this all-human platform integrates fully defined ECM, vasculature, and innervation in a single modular system to investigate tumor organization, invasion, and therapeutic responses within a controlled human microenvironment. FAU - Pignataro, Alessia Maria AU - Pignataro AM AUID- ORCID: 0009-0009-7432-5450 AD - Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Campus Valla, Linkoping 58330, Sweden. AD - MiMic Lab, Department of Electronics, Information and Bioengineering, Politecnico di Milano, Milano 20133, Italy. ROR: https://ror.org/01nffqt88 FAU - Schwarz, Cornelia Christina AU - Schwarz CC AUID- ORCID: 0009-0000-6851-6079 AD - Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Campus Valla, Linkoping 58330, Sweden. FAU - Wiechec, Emilia AU - Wiechec E AUID- ORCID: 0000-0003-0270-9374 AD - Division of Cell and Neurobiology, Department of Clinical and Biomedical Sciences, Linkoping University, Linkoping 58183, Sweden. ROR: https://ror.org/05ynxx418 AD - Region Ostergotland Anesthetics, Operations and Specialty Surgery Center, Department of Otorhinolaryngology, Linkoping 582 25, Sweden. ROR: https://ror.org/024emf479 FAU - Cordiale, Alessandro AU - Cordiale A AUID- ORCID: 0000-0003-2918-7131 AD - MiMic Lab, Department of Electronics, Information and Bioengineering, Politecnico di Milano, Milano 20133, Italy. ROR: https://ror.org/01nffqt88 FAU - Sasikumar, Shyama AU - Sasikumar S AUID- ORCID: 0009-0006-0108-3778 AD - Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Campus Valla, Linkoping 58330, Sweden. FAU - Jenssen, Alexander AU - Jenssen A AUID- ORCID: 0009-0008-3813-8335 AD - Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Campus Valla, Linkoping 58330, Sweden. FAU - Ariyaberg, Taka AU - Ariyaberg T AUID- ORCID: 0009-0001-2426-9667 AD - Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Campus Valla, Linkoping 58330, Sweden. FAU - Khare, Lalit Pramod AU - Khare LP AD - Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Campus Valla, Linkoping 58330, Sweden. FAU - Apu, Ehsanul Hoque AU - Apu EH AD - Bitonte College of Dentistry, Department of Biomedical Sciences, Northeast Ohio Medical University, Rootstown, OH 44272. ROR: https://ror.org/04q9qf557 FAU - Roberg, Karin AU - Roberg K AUID- ORCID: 0000-0003-1208-9746 AD - Division of Cell and Neurobiology, Department of Clinical and Biomedical Sciences, Linkoping University, Linkoping 58183, Sweden. ROR: https://ror.org/05ynxx418 AD - Region Ostergotland Anesthetics, Operations and Specialty Surgery Center, Department of Otorhinolaryngology, Linkoping 582 25, Sweden. ROR: https://ror.org/024emf479 FAU - Naeimipour, Sajjad AU - Naeimipour S AD - Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Campus Valla, Linkoping 58330, Sweden. FAU - Basile Carballo, Gabriela AU - Basile Carballo G AUID- ORCID: 0000-0003-4203-384X AD - Department of Biomedical and Clinical Sciences, Center for Social and Affective Neuroscience, Linkoping University, Linkoping 58183, Sweden. ROR: https://ror.org/05ynxx418 FAU - Szczot, Marcin AU - Szczot M AUID- ORCID: 0000-0002-3902-059X AD - Department of Biomedical and Clinical Sciences, Center for Social and Affective Neuroscience, Linkoping University, Linkoping 58183, Sweden. ROR: https://ror.org/05ynxx418 FAU - Aili, Daniel AU - Aili D AUID- ORCID: 0000-0002-7001-9415 AD - Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Campus Valla, Linkoping 58330, Sweden. FAU - Rasponi, Marco AU - Rasponi M AUID- ORCID: 0000-0002-2904-8652 AD - MiMic Lab, Department of Electronics, Information and Bioengineering, Politecnico di Milano, Milano 20133, Italy. ROR: https://ror.org/01nffqt88 FAU - Pagella, Pierfrancesco AU - Pagella P AUID- ORCID: 0000-0001-6912-0957 AD - Laboratory of Molecular Materials, Division of Biophysics and Bioengineering, Department of Physics, Chemistry and Biology, Campus Valla, Linkoping 58330, Sweden. LA - eng GR - 2025-03512/Vetenskapsradet (VR)/ GR - 2023-04675/Vetenskapsradet (VR)/ GR - KAW 2021.0186/Knut och Alice Wallenbergs Stiftelse (kawforskning)/ GR - 101044665 PROTECT/EC | European Research Council (ERC)/ GR - CCS23-0011/Swedish Foundation for Strategic Research/ GR - Linkoping University - Medical Inflammation and Infection Center MIIC/ PT - Journal Article DEP - 20261027 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 SB - IM MH - Humans MH - *Extracellular Matrix/metabolism MH - Tumor Microenvironment MH - *Squamous Cell Carcinoma of Head and Neck/pathology/blood supply/metabolism MH - Coculture Techniques MH - Microphysiological Systems MH - *Lab-On-A-Chip Devices MH - Tissue Engineering/methods MH - Cell Line, Tumor MH - *Head and Neck Neoplasms/pathology/blood supply/metabolism MH - Neovascularization, Pathologic MH - Cell Movement MH - Endothelial Cells OTO - NOTNLM OT - extracellular matrix OT - innervation OT - oral cancer OT - organ-on-a-chip OT - vasculature COIS- Competing interests statement:The authors declare no competing interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 13:43 PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:43 [entrez] AID - 10.1073/pnas.2602541123 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2026 Nov 3;123(44):e2602541123. doi: 10.1073/pnas.2602541123. Epub 2026 Oct 27. PMID- 42945255 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1091-6490 (Electronic) IS - 0027-8424 (Linking) VI - 123 IP - 44 DP - 2026 Nov 3 TI - Pancreatic delta cells are resistant to auto- and paracrine inhibition in human type-2 diabetes. PG - e2601168123 LID - 10.1073/pnas.2601168123 [doi] AB - Somatostatin secretion from pancreatic delta cells inhibits nearby alpha- and beta cells and tunes the body's glycemic set-point. The role of delta cells in diabetes remains unclear, in part due to the difficulty separating intrinsic regulation from intraislet paracrine effects. Here, we compared the function of isolated delta cells of cadaveric nondiabetic and type-2 diabetic donors by single cell total internal reflection microscopy, electrophysiology, and secretion measurements. Elevated glucose stimulated exocytosis of somatostatin, which was further amplified by glucagon, exendin-4, or forskolin, independent of diabetic status. gamma-aminobutyric acid (GABA) enhanced exocytosis and electrical activity, while insulin had no detectable effect. Adrenaline and somatostatin strongly inhibited delta cell activity, leading to autocrine feedback inhibition of somatostatin exocytosis. In type-2 diabetes, delta cell inhibition by somatostatin and adrenaline was impaired, together with a marked reduction in somatostatin receptor (SSTR2) surface expression. The data suggest that selective loss of inhibitory control of delta cells may dysregulate intraislet somatostatin signaling in type-2 diabetes. FAU - Omar-Hmeadi, Muhmmad AU - Omar-Hmeadi M AUID- ORCID: 0000-0001-8893-7348 AD - Department of Medical Cell Biology, Uppsala University, Uppsala 75123, Sweden. ROR: https://ror.org/048a87296 AD - Department of Animal Biosciences, Swedish University of Agricultural Sciences, Uppsala 75007, Sweden. ROR: https://ror.org/02yy8x990 FAU - Matuseviciene, Lina AU - Matuseviciene L AUID- ORCID: 0000-0002-6797-5078 AD - Department of Medical Cell Biology, Uppsala University, Uppsala 75123, Sweden. ROR: https://ror.org/048a87296 FAU - Liu, Liangwen AU - Liu L AD - Department of Medical Cell Biology, Uppsala University, Uppsala 75123, Sweden. ROR: https://ror.org/048a87296 FAU - Lund, Per-Eric AU - Lund PE AUID- ORCID: 0000-0002-5994-5290 AD - Department of Medical Cell Biology, Uppsala University, Uppsala 75123, Sweden. ROR: https://ror.org/048a87296 FAU - Barg, Sebastian AU - Barg S AUID- ORCID: 0000-0003-4661-5724 AD - Department of Medical Cell Biology, Uppsala University, Uppsala 75123, Sweden. ROR: https://ror.org/048a87296 LA - eng GR - na/Swedish Research Council/ GR - na/Diabetes Wellness Network Sweden/ GR - na/Swedish Diabetes Society/ GR - na/Barndiabetesfonden/ GR - na/European Foundation for the Study of Diabetes (EFSD)/ GR - na/NovoNordisk Foundation/ GR - na/Excellence of Diabetes Research in Sweden (EXODIAB)/ GR - na/Family Ernfors Foundation/ GR - na/OE&E Johanssons-Foundation/ PT - Journal Article DEP - 20261027 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 RN - 51110-01-1 (Somatostatin) RN - 9007-92-5 (Glucagon) RN - 0 (Receptors, Somatostatin) RN - YKH834O4BH (Epinephrine) RN - IY9XDZ35W2 (Glucose) RN - 0 (Insulin) RN - 56-12-2 (gamma-Aminobutyric Acid) RN - 0 (SSTR2 protein, human) RN - 1F7A44V6OU (Colforsin) RN - 9P1872D4OL (Exenatide) SB - IM MH - Humans MH - *Diabetes Mellitus, Type 2/metabolism/pathology MH - Somatostatin/metabolism MH - *Somatostatin-Secreting Cells/metabolism/drug effects/pathology MH - *Paracrine Communication/drug effects MH - Glucagon/metabolism/pharmacology MH - Receptors, Somatostatin/metabolism MH - Exocytosis/drug effects MH - Epinephrine/pharmacology MH - Glucose/metabolism/pharmacology MH - Insulin/metabolism MH - *Autocrine Communication MH - gamma-Aminobutyric Acid/pharmacology/metabolism MH - Colforsin/pharmacology MH - Exenatide/pharmacology MH - Female MH - Male MH - Insulin-Secreting Cells/metabolism OTO - NOTNLM OT - exocytosis OT - hormone secretion OT - pancreatic islets OT - somatostatin OT - type-2 diabetes COIS- Competing interests statement:The authors declare no competing interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 13:43 PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:43 [entrez] AID - 10.1073/pnas.2601168123 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2026 Nov 3;123(44):e2601168123. doi: 10.1073/pnas.2601168123. Epub 2026 Oct 27. PMID- 42945247 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261027 IS - 1091-6490 (Electronic) IS - 0027-8424 (Linking) VI - 123 IP - 44 DP - 2026 Nov 3 TI - Capture and reduction of singlet oxygen via an anthracene-based framework for stable Li-O(2) batteries. PG - e2628659123 LID - 10.1073/pnas.2628659123 [doi] AB - Given the increasing global energy demand and rapid advancement of low-carbon technologies, Li-O(2) batteries have attracted considerable attention owing to their exceptionally high theoretical energy density, offering great promise for advanced energy systems. However, the formation of harmful singlet oxygen ((1)O(2)) in both discharge and charge processes induces parasitic reactions that deteriorate round-trip efficiency and cycling stability. Herein, an anthracene-based covalent-organic framework (AntCOF) is introduced as a highly effective catalyst for the (1)O(2) reduction reaction (SORR) in Li-O(2) batteries. The conjugated porous framework selectively captures electrophilic (1)O(2), enabling effective charge transfer from AntCOF to the empty pi* orbital of (1)O(2) and promoting SORR in subsequent discharge processes. This mechanism suppresses (1)O(2) diffusion and mitigates associated side reactions, leading to reduced overpotentials and improved cycling performance when coupled with a RuO(2) catalyst. This work may enlighten further investigations into (1)O(2)-related chemistry across a broad range of oxygen-redox electrochemical systems. FAU - Jiang, Zhuoliang AU - Jiang Z AD - Academy for Advanced Interdisciplinary Studies, Frontiers Science Center for New Organic Matter, State Key Laboratory of Advanced Chemical Power Sources, Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education), College of Chemistry, Nankai University, Tianjin 300071, China. AD - State Key Laboratory of Tropic Ocean Engineering Materials and Materials Evaluation, School of Materials Science and Engineering, Hainan Provincial Key Lab of Fine Chem, Hainan University, Haikou 570228, China. FAU - Zhao Zhao AU - Zhao Zhao AD - State Key Laboratory of Tropic Ocean Engineering Materials and Materials Evaluation, School of Materials Science and Engineering, Hainan Provincial Key Lab of Fine Chem, Hainan University, Haikou 570228, China. FAU - Gao, Liang AU - Gao L AD - Jinan Key Laboratory of High Performance Industrial Software, Jinan Institute of Supercomputing Technology, Jinan 250103, China. FAU - Ma, Yifei AU - Ma Y AD - State Key Laboratory of Tropic Ocean Engineering Materials and Materials Evaluation, School of Materials Science and Engineering, Hainan Provincial Key Lab of Fine Chem, Hainan University, Haikou 570228, China. FAU - Liu, Ke AU - Liu K AD - State Key Laboratory of Tropic Ocean Engineering Materials and Materials Evaluation, School of Materials Science and Engineering, Hainan Provincial Key Lab of Fine Chem, Hainan University, Haikou 570228, China. FAU - An, Jiaxuan AU - An J AD - Academy for Advanced Interdisciplinary Studies, Frontiers Science Center for New Organic Matter, State Key Laboratory of Advanced Chemical Power Sources, Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education), College of Chemistry, Nankai University, Tianjin 300071, China. FAU - Huang, Yaohui AU - Huang Y AUID- ORCID: 0000-0002-9037-8263 AD - Academy for Advanced Interdisciplinary Studies, Frontiers Science Center for New Organic Matter, State Key Laboratory of Advanced Chemical Power Sources, Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education), College of Chemistry, Nankai University, Tianjin 300071, China. FAU - Chen, Zikang AU - Chen Z AD - Academy for Advanced Interdisciplinary Studies, Frontiers Science Center for New Organic Matter, State Key Laboratory of Advanced Chemical Power Sources, Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education), College of Chemistry, Nankai University, Tianjin 300071, China. FAU - Tian, Xinlong AU - Tian X AUID- ORCID: 0000-0001-8388-5198 AD - State Key Laboratory of Tropic Ocean Engineering Materials and Materials Evaluation, School of Materials Science and Engineering, Hainan Provincial Key Lab of Fine Chem, Hainan University, Haikou 570228, China. FAU - Li, Fujun AU - Li F AUID- ORCID: 0000-0002-1298-0267 AD - Academy for Advanced Interdisciplinary Studies, Frontiers Science Center for New Organic Matter, State Key Laboratory of Advanced Chemical Power Sources, Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education), College of Chemistry, Nankai University, Tianjin 300071, China. LA - eng GR - 2024YFA1211100/National Key R&D Program of China/ GR - 22325902/National Natural Science Foundation of China/ GR - 52171215/National Natural Science Foundation of China/ GR - JYB2025XDXM410/Fundamental and Interdisciplinary Disciplines Breakthrough Plan of the Ministry of Education of China/ GR - XJ2500000502/Start-up Research Foundation of Hainan University/ PT - Journal Article DEP - 20261027 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 SB - IM OTO - NOTNLM OT - COF OT - Li-O2 batteries OT - ORR OT - parasite reaction OT - singlet oxygen COIS- Competing interests statement:The authors declare no competing interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 13:43 PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:43 [entrez] AID - 10.1073/pnas.2628659123 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2026 Nov 3;123(44):e2628659123. doi: 10.1073/pnas.2628659123. Epub 2026 Oct 27. PMID- 42945239 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1091-6490 (Electronic) IS - 0027-8424 (Linking) VI - 123 IP - 44 DP - 2026 Nov 3 TI - Revised estimates of types and duration of long COVID symptoms based on claims records from 245 million US patients. PG - e2604414123 LID - 10.1073/pnas.2604414123 [doi] AB - COVID-19 has been reported to cause multisystem effects lasting years [Z. Al-Aly, E. Topol, Science 383, 830-832 (2024)]. These findings are primarily based on cohort studies comparing test-positive COVID-19 patients to controls without known COVID-19 infection, tested or untested [M. Cai, Y. Xie, E. J. Topol, Z. Al-Aly, Nat. Med. 30, 1564-1573 (2024)]. However, such comparisons can be biased by the reasons people were tested. Using 14.4 billion insurance claims from 244.7 million US patients, we find that most long-term effects attributed to COVID-19 by unrestricted control designs likely reflect bias due to selective testing of individuals with non-COVID conditions, causing long-term effects of those conditions to be misattributed to COVID-19. We removed this bias by analyzing only tested individuals, comparing test-positive to test-negative patients. Across 614 clinical outcomes followed over 2 y, the test-based design resulted in 83.5% fewer symptoms being attributable to COVID-19 between 30 to 119 d after infection (43 attributable symptoms vs. 262 for the unrestricted control design), a 98.2% reduction at 120 to 359 d (5 vs. 286), and a 100% reduction at 360 to 719 d (0 vs. 369). The unrestricted control design also attributed unrelated outcomes (e.g., firearm injuries) to COVID-19 at a high rate, whereas the testing-based design did not. The test-based control design may modestly underestimate some effects. This study does not indicate that symptoms beyond one year are not genuine but that their attributability to COVID-19 is uncertain. FAU - Nilforoshan, Hamed AU - Nilforoshan H AD - Department of Computer Science, Stanford University, Stanford, CA 94305. ROR: https://ror.org/00f54p054 FAU - Reisler, Julia AU - Reisler J AD - Department of Computer Science, Stanford University, Stanford, CA 94305. ROR: https://ror.org/00f54p054 FAU - Jahanparast, Erfan AU - Jahanparast E AUID- ORCID: 0009-0007-4599-9355 AD - Department of Computer Science, University of California, Berkeley, CA 94720. ROR: https://ror.org/01an7q238 FAU - Moor, Michael AU - Moor M AD - Department of Computer Science, Stanford University, Stanford, CA 94305. ROR: https://ror.org/00f54p054 AD - Department of Biosystems Science and Engineering, ETH Zurich, Basel 4056, Switzerland. ROR: https://ror.org/05a28rw58 FAU - Goodman, Steven N AU - Goodman SN AUID- ORCID: 0000-0002-3872-5723 AD - Department of Epidemiology and Population Health, Stanford University, Stanford, CA 94305. ROR: https://ror.org/00f54p054 FAU - Wager, Stefan AU - Wager S AUID- ORCID: 0000-0002-7526-9077 AD - Stanford Graduate School of Business, Stanford University, Stanford, CA 94305. ROR: https://ror.org/00f54p054 FAU - Leskovec, Jure AU - Leskovec J AUID- ORCID: 0000-0002-5411-923X AD - Department of Computer Science, Stanford University, Stanford, CA 94305. ROR: https://ror.org/00f54p054 LA - eng GR - DGE-1656518/NSF (NSF)/ PT - Journal Article DEP - 20261027 PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 SB - IM MH - Humans MH - *COVID-19/epidemiology/diagnosis MH - United States/epidemiology MH - SARS-CoV-2/isolation & purification MH - Post-Acute COVID-19 Syndrome MH - Time Factors MH - Female OTO - NOTNLM OT - causal inference OT - epidemiology OT - long COVID COIS- Competing interests statement:The authors declare no competing interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 13:43 PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:43 [entrez] AID - 10.1073/pnas.2604414123 [doi] PST - ppublish SO - Proc Natl Acad Sci U S A. 2026 Nov 3;123(44):e2604414123. doi: 10.1073/pnas.2604414123. Epub 2026 Oct 27. PMID- 42945230 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1399-0012 (Electronic) IS - 0902-0063 (Linking) VI - 40 IP - 10 DP - 2026 Oct TI - Vascular Management Strategies and Outcomes of Infectious Arterial Complications Involving the Kidney Transplant Vasculature: A Systematic Review and Evidence-Informed Treatment Framework. LID - e70734 [pii] LID - 10.1111/ctr.70734 [doi] AB - OBJECTIVE: Infectious arterial complications involving the kidney transplant (KT) vasculature are rare but life-threatening and frequently result in KT loss. The lack of evidence-based guidance for vascular management strategies underscores the need for systematic synthesis to inform clinical decision-making. METHODS: A systematic search was performed from database inception through August 22, 2025, for reports of culture-proven infectious vascular complications-including arterial rupture, detachment, pseudoaneurysm, aneurysm, thrombosis, or stenosis-involving the transplant renal artery, iliac vessels, or arterial anastomosis in KT recipients. Eligible studies reported vascular management strategies. Due to methodological heterogeneity, findings were synthesized narratively using proportion-based comparisons. RESULTS: Eighty reports (1972-2025) comprising 139 cases were included. Candida species were most frequent, followed by Pseudomonas, Aspergillus, and Staphylococcus species. Pseudoaneurysm and arterial rupture were the predominant clinical presentation, typically occuring within the first month after transplantation. Outcome patterns differed by transplant nephrectomy status, with vascular management strategies performed without transplant nephrectomy demonstrating higher reported proportions of uneventful follow-up. Autologous interposition grafting demonstrated the most favorable outcome pattern (86.7% uneventful recovery; 6.7% reintervention and mortality), even when transplant nephrectomy is unavoidable. Covered stent placement was the most frequently reported intervention andshowed favorable outcomes in selected KT-preserving cases (75% uneventful recovery). Primary surgical repair had the highest reintervention proportion (59.1%), whereas arterial ligation had the highest mortality (43.8%) and accounted for all reported limb loss. CONCLUSION: Across five decades of case-based evidence, autologous interposition grafting demonstrated the most favorable outcome pattern and may represent the preferred vascular management strategy when durable arterial reconstruction is feasible, including when transplant nephrectomy is required. Covered stent placement may provide a useful option in selected cases or as a temporizing strategy for hemorrhage control. These findings should guide rather than define management decisions given the heterogeneous evidence base. HIGHLIGHTS: First systematic synthesis of management strategies for infectious arterial complications involving the kidney transplant vasculature Candida was the most frequently reported pathogen; pseudoaneurysm and arterial rupture were the predominant presentations Vascular management strategies performed without transplant nephrectomy demonstrated higher reported proportions of uneventful follow-up Autologous interposition grafting showed the most favorable reported outcome pattern, even when transplant nephrectomy is unavoidable Ligation and primary repair were associated with higher reported mortality and reintervention, respectively. CI - (c) 2026 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. FAU - Mozafar, Mehrdad AU - Mozafar M AD - Division of Vascular and Endovascular Surgery, Department of Surgery, Shohada-Tajrish Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. AD - School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. FAU - Bigdeli, Yasamin AU - Bigdeli Y AD - Department of Surgery, Surgery Research Center, School of Medicine, Rasool-E Akram Hospital, Iran University of Medical Sciences, Tehran, Iran. FAU - Lichani, Mohammad Amouzadeh AU - Lichani MA AD - Student Research Committee, School of Medicine, Anzali International Campus, Guilan University of Medical Sciences, Rasht, Iran. FAU - Looha, Mehdi Azizmohammad AU - Looha MA AD - Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute For Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran. FAU - Mozafar, Mohamad AU - Mozafar M AD - Division of Vascular and Endovascular Surgery, Department of Surgery, Shohada-Tajrish Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. FAU - Modarai, Bijan AU - Modarai B AD - Department of Vascular Surgery, Guy's & St Thomas' NHS Foundation Trust, London, Greater London, UK. FAU - Lyon, Oliver AU - Lyon O AD - Surgery and Critical Care, University of Otago, Christchurch, New Zealand. FAU - Yi, Stephanie AU - Yi S AD - Department of Surgery, J.C. Walter Jr. Transplant Center, Houston Methodist Hospital, Houston, Texas, USA. FAU - Kagan, Anna AU - Kagan A AD - Division of Nephrology, Department of Internal Medicine, Houston Methodist Hospital, Houston, Texas, USA. FAU - Grimes, Kevin A AU - Grimes KA AD - Division of Infectious Diseases, Department of Medicine, Houston Methodist Hospital, Houston, Texas, USA. FAU - Rahimi, Maham AU - Rahimi M AD - Department of Cardiovascular Surgery, Houston Methodist Hospital, Houston, Texas, USA. LA - eng PT - Journal Article PT - Review PT - Systematic Review PL - Denmark TA - Clin Transplant JT - Clinical transplantation JID - 8710240 SB - IM MH - Humans MH - *Kidney Transplantation/adverse effects MH - *Postoperative Complications/therapy/etiology MH - Prognosis MH - *Vascular Surgical Procedures/methods MH - Evidence-Based Medicine MH - *Vascular Diseases/etiology/therapy OTO - NOTNLM OT - endovascular procedures OT - kidney transplantation OT - mycotic pseudoaneurysm OT - nephrectomy OT - vascular complications EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 13:43 PHST- 2026/10/08 00:00 [revised] PHST- 2026/04/04 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:43 [entrez] AID - e70734 [pii] AID - 10.1111/ctr.70734 [doi] PST - ppublish SO - Clin Transplant. 2026 Oct;40(10):e70734. doi: 10.1111/ctr.70734. PMID- 42945229 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - On the variability of generative artificial intelligence methods in conceptual modeling: an experimental evaluation on combining causal maps. PG - e0361281 LID - 10.1371/journal.pone.0361281 [doi] LID - e0361281 AB - Large Language Models (LLMs) increasingly support time-consuming conceptual modeling tasks such as building or combining models from text. In this context, causal maps are labeled, directed graphs that represent causal relationships (edges) between selected factors (nodes) in a problem domain. Modelers control the output through the LLM's prompt and decoding parameters such as temperature, and recent studies either use a default temperature or minimize it by expecting that it supports replicability. However, evidence about this expectation remains limited, as does our understanding of how different LLMs and decoding or prompting strategies shape results. We assess the extent to which findings are an artifact of the LLM or its variability rather than a reflection of the modeler's controls. We focus on the common conceptual modeling task of combining models, exemplified through three algorithms that reflect a variety of prompting techniques. We use five LLMs (DeepSeek, Gemini, GPT, Qwen, Ministral) and different versions to account for the potential impact of the architectures. Using the Design of Experiments approach of factorial analyses (supplemented by paired tests and confidence interval checks), our results show that outcomes differ significantly across LLMs and versions. Depending on the algorithm, randomness from the LLM can be the dominating factor, responsible for up to 96% of the variation in results. This happens even if we directly control the decoding, as evidenced by our comparison of greedy, beam-search, and contrastive decoding. We find that prompt effects are systematic but metric-dependent, while decoding affects performance but there is no universally best strategy. Since our findings suggest that replicability of conceptual modeling studies using LLMs may be limited, future studies may report the distribution of performance measures alongside exact model, decoding, replication, and baseline-normalization settings. CI - Copyright: (c) 2026 Flandre et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Flandre, Noe Y AU - Flandre NY AD - Old Dominion University (ODU) Virginia Modeling, Analysis, and Simulation Center (VMASC), Suffolk, Virginia, United States of America. AD - School of Mines in Ales (IMT Mines Ales), Ales, France. FAU - Daumas, Cedric AU - Daumas C AD - Old Dominion University (ODU) Virginia Modeling, Analysis, and Simulation Center (VMASC), Suffolk, Virginia, United States of America. AD - School of Mines in Ales (IMT Mines Ales), Ales, France. FAU - Giabbanelli, Philippe J AU - Giabbanelli PJ AUID- ORCID: 0000-0001-6816-355X AD - Old Dominion University (ODU) Virginia Modeling, Analysis, and Simulation Center (VMASC), Suffolk, Virginia, United States of America. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - *Generative Artificial Intelligence MH - Large Language Models MH - Algorithms MH - Humans PMC - PMC13712584 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:43 PHST- 2025/10/07 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:43 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PONE-D-25-54419 [pii] AID - 10.1371/journal.pone.0361281 [doi] PST - epublish SO - PLoS One. 2026 Oct 27;21(10):e0361281. doi: 10.1371/journal.pone.0361281. eCollection 2026. PMID- 42945228 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - The effect of dual task-oriented circuit training on neuroplasticity and walking adaptability in subacute stroke patients: A pilot and feasibility study. PG - e0361255 LID - 10.1371/journal.pone.0361255 [doi] LID - e0361255 AB - BACKGROUND: Stroke remains a leading cause of long-term disability worldwide, with a substantial proportion of survivors experiencing persistent limitations in functional mobility. Although many individuals regain the ability to walk in structured clinical environments, only a minority achieve independent community ambulation due to cognitive-motor interference (CMI). The subacute phase is characterized by heightened neuroplastic potential and responsiveness to rehabilitation. Dual Task-Oriented Circuit Training (DTOCT) integrates task-oriented circuit training with concurrent cognitive challenges and may provide a functionally relevant approach to addressing cognitive-motor interference. However, its feasibility, safety, and preliminary effects in subacute stroke remain unclear. METHODS: This three-arm, participant-blinded, randomized pilot and feasibility trial will recruit 36 individuals with subacute stroke and allocate them equally to DTOCT, Task-Oriented Circuit Training (TOCT), or Dual Task Training (DTT). Participants will receive three supervised sessions per week for 4 weeks (12 sessions). Primary outcomes will assess feasibility and safety, including adherence, retention, adverse events, heart rate, blood pressure, and perceived exertion. Secondary exploratory outcomes will include walking endurance assessed using the 6-Minute Walk Test, gait speed using the 10-Meter Walk Test, walking adaptability using the Standardized Walking Obstacle Course, and serum brain-derived neurotrophic factor as an exploratory peripheral biomarker associated with neuroplasticity. DISCUSSION: This pilot study is designed to determine the feasibility and safety of DTOCT and to generate preliminary effect estimates for clinical and neurobiological outcomes. The findings will inform primary outcome selection, intervention refinement, and sample-size estimation for a subsequent adequately powered randomized controlled trial rather than establish definitive efficacy or superiority. TRIAL REGISTRATION: ClinicalTrials.gov. Identifier: NCT07494357 (registered prospectively). Protocol ID: IKFR-202603.01). CI - Copyright: (c) 2026 Arisanti et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Arisanti, Farida AU - Arisanti F AUID- ORCID: 0000-0001-6427-6178 AD - Faculty of Medicine, Doctoral Study Program in Medical Science, Universitas Padjadjaran, Kabupaten Sumedang, Indonesia. AD - Department of Physical and Rehabilitation Medicine, Faculty of Medicine Universitas Padjadjaran, Kabupaten Sumedang, Indonesia. FAU - Ropii, Bejo AU - Ropii B AD - Preanger Institute for Biomedical Engineering, Science, and Technology, Bandung, Indonesia. FAU - Ghaida, Saniy AU - Ghaida S AD - Kabupaten Sumedang, Physical Medicine and Rehabilitation Study Program, Faculty of Medicine, Universitas Padjadjdaran, Kabupaten Sumedang, Indonesia. FAU - Tristiana Dewi, Tsuchiya AU - Tristiana Dewi T AD - Kabupaten Sumedang, Physical Medicine and Rehabilitation Study Program, Faculty of Medicine, Universitas Padjadjdaran, Kabupaten Sumedang, Indonesia. FAU - Poernomo, Bernadeta Cahyo Setianingrum AU - Poernomo BCS AD - Dustira Hospital, Bandung, Indonesia. FAU - Sribudiani, Yunia AU - Sribudiani Y AD - Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Kabupaten Sumedang, Indonesia. FAU - Amalia, Lisda AU - Amalia L AD - Department of Neurology, Faculty of Medicine, Universitas Padjadjaran, Kabupaten Sumedang, Indonesia. FAU - Engkasan, Julia Patrick AU - Engkasan JP AUID- ORCID: 0000-0003-0599-4908 AD - Department of Rehabilitation Medicine, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia. FAU - Biben, Vitriana AU - Biben V AUID- ORCID: 0000-0002-0729-8853 AD - Department of Physical and Rehabilitation Medicine, Faculty of Medicine Universitas Padjadjaran, Kabupaten Sumedang, Indonesia. LA - eng SI - ClinicalTrials.gov/NCT07494357 PT - Journal Article PT - Randomized Controlled Trial DEP - 20261027 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Adult MH - Aged MH - Female MH - Humans MH - Male MH - Middle Aged MH - *Circuit-Based Exercise/methods MH - *Dual-Task Tests MH - Feasibility Studies MH - *Neuronal Plasticity/physiology MH - Pilot Projects MH - *Stroke/physiopathology/therapy MH - *Stroke Rehabilitation/methods MH - *Walking/physiology PMC - PMC13712583 COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:43 PHST- 2026/07/10 00:00 [received] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:43 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PONE-D-26-30990 [pii] AID - 10.1371/journal.pone.0361255 [doi] PST - epublish SO - PLoS One. 2026 Oct 27;21(10):e0361255. doi: 10.1371/journal.pone.0361255. eCollection 2026. PMID- 42945225 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Mechanistic insights into ENO2-driven glycolysis and tumor progression in hepatocellular carcinoma. PG - e0361057 LID - 10.1371/journal.pone.0361057 [doi] LID - e0361057 AB - BACKGROUND AND AIMS: Neuron-specific enolase 2 (ENO2) has been implicated in tumor metabolism, but its biological role in hepatocellular carcinoma remains incompletely defined. This study aimed to investigate the expression, prognostic relevance, and functional role of ENO2 in HCC. METHODS: Public transcriptomic datasets, RNA sequencing, WGCNA, survival analyses, and pathway enrichment analyses were integrated to identify HCC-associated genes. ENO2 expression was validated in HCC cell lines and paired clinical tissues.Gain- and loss-of-function, catalytic-rescue, glycolytic-inhibition, and pyruvate-rescue experiments were performed to evaluate glycolytic activity, proliferation, colony formation, migration, invasion, and tumor growth. RESULTS: ENO2 was upregulated in HCC and was associated with advanced tumor stage and unfavorable survival outcomes in public datasets. ENO2 overexpression increased glycolytic activity and malignant phenotypes, whereas ENO2 depletion produced opposite effects. In ENO2-depleted cells, re-expression of shRNA-resistant ENO2 wild type restored the impaired glycolytic and malignant phenotypes, whereas the catalytic-deficient ENO2 mutant failed to restore them. Moreover, 2-deoxy-D-glucose attenuated ENO2 overexpression-associated phenotypes, while pyruvate partially rescued phenotypes associated with ENO2 depletion. ENO2 expression was also associated with altered PI3K and AKT phosphorylation. CONCLUSIONS: ENO2 may contribute to HCC progression by enhancing glycolytic metabolism and malignant phenotypes, partly in association with PI3K/AKT signaling. ENO2 is a candidate metabolic and prognostic biomarker that requires validation in larger independent clinical cohorts and warrants further investigation as a therapeutic candidate. CI - Copyright: (c) 2026 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Liu, Yan AU - Liu Y AD - Guizhou Provincial People's Hospital, Department of Hepatobiliary Surgery, Guiyang, Guizhou Province, China. FAU - Zhang, Xiao-Ran AU - Zhang XR AD - Guizhou Medical University, School of Basic Medical Sciences, Anshun, Guizhou Province, China. FAU - Dai, Li AU - Dai L AD - Department of Surgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou Province, China. FAU - Li, Hai-Liang AU - Li HL AD - Department of Surgery, The First People's Hospital of Zunyi, The Third Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou Province, China. FAU - Jin, Xiang-Ren AU - Jin XR AD - Department of Surgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou Province, China. FAU - Qi, Hong-Bin AU - Qi HB AD - Guizhou Medical University, School of Basic Medical Sciences, Anshun, Guizhou Province, China. FAU - Tang, Ke AU - Tang K AUID- ORCID: 0009-0000-1358-6627 AD - Guiyang Nanming District People's Hospital, Department of Surgery, Guizhou Province, China. FAU - Li, Qiang AU - Li Q AD - Guizhou Provincial People's Hospital, Department of Hepatobiliary Surgery, Guiyang, Guizhou Province, China. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - EC 4.2.1.11 (Phosphopyruvate Hydratase) RN - EC 4.2.1.11 (ENO2 protein, human) RN - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) SB - IM MH - Humans MH - *Phosphopyruvate Hydratase/metabolism/genetics MH - *Glycolysis MH - *Liver Neoplasms/pathology/metabolism/genetics MH - *Carcinoma, Hepatocellular/pathology/metabolism/genetics MH - Cell Line, Tumor MH - Gene Expression Regulation, Neoplastic MH - Disease Progression MH - Cell Proliferation MH - Signal Transduction MH - Animals MH - Prognosis MH - Female MH - Proto-Oncogene Proteins c-akt/metabolism MH - Cell Movement MH - Male PMC - PMC13712540 COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:43 PHST- 2026/07/15 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:43 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PONE-D-26-32844 [pii] AID - 10.1371/journal.pone.0361057 [doi] PST - epublish SO - PLoS One. 2026 Oct 27;21(10):e0361057. doi: 10.1371/journal.pone.0361057. eCollection 2026. PMID- 42945213 OWN - NLM STAT- Publisher LR - 20261027 IS - 2211-1247 (Electronic) VI - 45 IP - 11 DP - 2026 Oct 26 TI - The compositional encoding of hand-eye coordinated movements for single neurons in the posterior parietal cortex. PG - 118204 LID - S2211-1247(26)01283-0 [pii] LID - 10.1016/j.celrep.2026.118204 [doi] AB - Human posterior parietal cortex (PPC) is known to play a role in hand-eye (H-E) coordination, yet the underlying encoding mechanisms remain uncertain. We recorded 412 single neurons across 11 sessions from motor cortex (MC; n = 251) and PPC (n = 161) in a single human participant performing an H-E coordinated center-out task. While MC neurons showed negligible modulation by eye movements, 79% of PPC neurons had neural representations that were additively separable into independent hand- and eye-movement tuning curves. Due to this separability, representations could be separated and additively recomposed while maintaining structural similarity. Consequently, compositional decoders trained solely on single-effector movements could match the performance of decoders trained on coordinated H-E movements (hand: 66% vs. 69%; eye: 34% vs. 36%). These results show that, during simple center-out tasks, MC hand-movement codes are unaffected by eye movements and that compositionality can be used to modularly decode H-E coordinated movements in PPC. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Mynhier, Nikos A AU - Mynhier NA AD - Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. Electronic address: nmynhier@caltech.edu. FAU - Gamez, Jorge AU - Gamez J AD - Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. FAU - Moorse, Kellan P AU - Moorse KP AD - Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. FAU - Pejsa, Kelsie AU - Pejsa K AD - Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. FAU - Bari, Ausaf AU - Bari A AD - Department of Neurosurgery, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA. FAU - Murray, Richard M AU - Murray RM AD - Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA; Division of Engineering and Applied Science, California Institute of Technology, Pasadena, CA 91125, USA. FAU - Andersen, Richard A AU - Andersen RA AD - Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. Electronic address: richard.andersen@vis.caltech.edu. LA - eng PT - Journal Article DEP - 20261026 PL - United States TA - Cell Rep JT - Cell reports JID - 101573691 SB - IM OTO - NOTNLM OT - CP: neuroscience OT - brain-computer interface OT - brain-machine interface OT - compositionality OT - hand-eye coordination OT - mixed selectivity OT - motor cortex OT - neural decoding OT - posterior parietal cortex OT - single-neuron recordings OT - superior parietal lobule COIS- Declaration of interests Richard M. Murray serves on the board of Convergent. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 13:35 PHST- 2026/04/13 00:00 [received] PHST- 2026/08/06 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:35 [entrez] AID - S2211-1247(26)01283-0 [pii] AID - 10.1016/j.celrep.2026.118204 [doi] PST - aheadofprint SO - Cell Rep. 2026 Oct 26;45(11):118204. doi: 10.1016/j.celrep.2026.118204. PMID- 42945199 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1553-7358 (Electronic) IS - 1553-734X (Print) IS - 1553-734X (Linking) VI - 22 IP - 10 DP - 2026 Oct TI - Evolutionary and dynamic constraints converge on a conserved coupling sector in thermally adapted GH1 beta-glucosidases. PG - e1014879 LID - 10.1371/journal.pcbi.1014879 [doi] LID - e1014879 AB - How protein sequences evolve to maintain functional conformational dynamics under thermal stress remains an important question in molecular evolution. Thermophilic enzymes are often considered to achieve stability primarily through enhanced structural rigidity, yet thermal adaptation may also involve the preservation or reorganization of correlated motions across the protein scaffold. Here, we compared three glycoside hydrolase family 1 beta-glucosidases-BsBgl1A, 9UPT, and LQBG8-that share a conserved (beta/alpha)8 TIM-barrel fold but display distinct optimal temperatures of approximately 45, 65, and 70 degrees C, respectively. By integrating comparative structural analysis, three independent 1 mus molecular dynamics simulations at 300, 325, and 350 K, neural relational inference of residue-level dynamic coupling, and family-level coevolutionary analysis, we identified distinct dynamic strategies associated with their thermal phenotypes. All three enzymes retained their global compactness and principal secondary-structure framework across the simulated temperatures, indicating that differences in RMSD and residue flexibility did not reflect large-scale unfolding. BsBgl1A showed the strongest temperature sensitivity, with increased structural deviation and residue mobility at 350 K. Its inferred interaction network exhibited a non-monotonic response, characterized by pronounced overcoupling at 325 K followed by reduced connectivity at 350 K. In contrast, 9UPT maintained relatively restrained residue dynamics and a highly reproducible coupling topology across temperatures. LQBG8 combined substantial local conformational flexibility with persistent long-range coupling and retained the highest directed network density at 350 K. Across the three enzymes, the betaalpha5-betaalpha7 region repeatedly emerged as a shared structural and dynamic center. Coevolutionary couplings were also enriched in TIM-barrel-associated regions that spatially converged on this sector. These findings indicate that thermal adaptation in GH1 beta-glucosidases is not adequately explained by uniform structural rigidification. Instead, enzymes with the same conserved fold may adopt distinct organizations of correlated motion, while greater thermal adaptation is associated with the persistence or reorganization of a betaalpha5-betaalpha7-centered dynamic coupling pattern under elevated temperature. The correspondence between dynamic coupling and coevolutionary enrichment is consistent with long-term evolutionary constraint on this structural sector, although it does not establish direct selection on the network as an independent functional unit. Our results therefore provide a testable framework in which thermostability reflects a balance among local stabilization, regional flexibility, and the resilience of higher-order dynamic organization. CI - Copyright: (c) 2026 Zheng et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Zheng, Li-Ting AU - Zheng LT AUID- ORCID: 0009-0001-3867-5156 AD - College of Pharmacy, Dali University, Dali, China. AD - College of Agriculture and Biological Science, Dali University, Dali, China. AD - Key Laboratory of Bioinformatics and Computational Biology, Department of Education of Yunnan Province, Dali University, Dali, China. AD - Co-Innovation Center for Cangshan Mountain and Erhai Lake Integrated Protection and Green Development of Yunnan Province, Dali University, Dali, China. FAU - Li, Yi AU - Li Y AD - Key Laboratory of Bioinformatics and Computational Biology, Department of Education of Yunnan Province, Dali University, Dali, China. AD - Co-Innovation Center for Cangshan Mountain and Erhai Lake Integrated Protection and Green Development of Yunnan Province, Dali University, Dali, China. AD - College of Mathematics and Computer Science, Dali University, Dali, China. FAU - He, Xian AU - He X AD - College of Agriculture and Biological Science, Dali University, Dali, China. AD - Key Laboratory of Bioinformatics and Computational Biology, Department of Education of Yunnan Province, Dali University, Dali, China. AD - Co-Innovation Center for Cangshan Mountain and Erhai Lake Integrated Protection and Green Development of Yunnan Province, Dali University, Dali, China. FAU - Yang, Lian-Tao AU - Yang LT AD - College of Mathematics and Computer Science, Dali University, Dali, China. FAU - Zhao, De-Rui AU - Zhao DR AD - College of Agriculture and Biological Science, Dali University, Dali, China. AD - Key Laboratory of Bioinformatics and Computational Biology, Department of Education of Yunnan Province, Dali University, Dali, China. AD - Co-Innovation Center for Cangshan Mountain and Erhai Lake Integrated Protection and Green Development of Yunnan Province, Dali University, Dali, China. FAU - Liu, Meng-Ting AU - Liu MT AD - College of Pharmacy, Dali University, Dali, China. AD - Key Laboratory of Bioinformatics and Computational Biology, Department of Education of Yunnan Province, Dali University, Dali, China. AD - Co-Innovation Center for Cangshan Mountain and Erhai Lake Integrated Protection and Green Development of Yunnan Province, Dali University, Dali, China. FAU - Sang, Peng AU - Sang P AD - College of Agriculture and Biological Science, Dali University, Dali, China. AD - Key Laboratory of Bioinformatics and Computational Biology, Department of Education of Yunnan Province, Dali University, Dali, China. AD - Co-Innovation Center for Cangshan Mountain and Erhai Lake Integrated Protection and Green Development of Yunnan Province, Dali University, Dali, China. FAU - Yang, Li-Quan AU - Yang LQ AD - College of Agriculture and Biological Science, Dali University, Dali, China. AD - Key Laboratory of Bioinformatics and Computational Biology, Department of Education of Yunnan Province, Dali University, Dali, China. AD - Co-Innovation Center for Cangshan Mountain and Erhai Lake Integrated Protection and Green Development of Yunnan Province, Dali University, Dali, China. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLoS Comput Biol JT - PLoS computational biology JID - 101238922 RN - EC 3.2.1.21 (beta-Glucosidase) RN - EC 3.2.1.- (Cellulases) SB - IM MH - Molecular Dynamics Simulation MH - *Evolution, Molecular MH - *beta-Glucosidase/chemistry/genetics/metabolism MH - Protein Conformation MH - Temperature MH - Enzyme Stability MH - *Cellulases/chemistry/genetics PMC - PMC13712516 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:34 PHST- 2026/02/04 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:34 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PCOMPBIOL-D-26-00283 [pii] AID - 10.1371/journal.pcbi.1014879 [doi] PST - epublish SO - PLoS Comput Biol. 2026 Oct 27;22(10):e1014879. doi: 10.1371/journal.pcbi.1014879. eCollection 2026 Oct. PMID- 42945196 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1553-7404 (Electronic) IS - 1553-7390 (Print) IS - 1553-7390 (Linking) VI - 22 IP - 10 DP - 2026 Oct TI - The peculiar properties of pericentromeric regions of genomes and their influence on sex chromosome evolution. PG - e1012333 LID - 10.1371/journal.pgen.1012333 [doi] LID - e1012333 AB - Results from recent genome sequences support the idea that the lack of recombination across extensive sex-linked regions (SLRs) in some species may be a pre-existing feature, due mainly to the sex-determining locus being located near a centromere. This would cause it to be affected by the "centromere-effect", which ensures low recombination rates near centromeres. In such cases, it is unnecessary to invoke any active recombination suppression process, or to debate ideas about the types of selection involved. In this review, I outline the properties of genome regions that recombine rarely, including pericentromeric regions of chromosomes, and discuss SLRs that overlap with pericentromeric regions. Unsurprisingly, it is difficult to distinguish special features of such SLRs that would suggest they have undergone a recombination suppression process. The property most likely to be specific to completely SLRs is major gene loss from Y- or W-linked regions of genes found in their respective X- or Z-linked counterparts, and this asymmetry is observed in many species. However, if pericentromeric regions are characterised by ephemeral gene content and low gene expression, asymmetry between pericentromeric SLRs might be less pronounced than in SLRs in other genome regions, and similar Y and X degeneration levels are indeed documented in several species. Additionally, some such SLRs may not be completely sex-linked, which could explain findings of extensive SLRs with low Y-X (or W-Z) divergence. CI - Copyright: (c) 2026 Deborah Charlesworth. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Charlesworth, Deborah AU - Charlesworth D AUID- ORCID: 0000-0002-3939-9122 AD - Institute of Ecology and Evolution, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - United States TA - PLoS Genet JT - PLoS genetics JID - 101239074 SB - IM MH - *Centromere/genetics MH - *Evolution, Molecular MH - Recombination, Genetic MH - Animals MH - *Sex Chromosomes/genetics MH - Humans MH - *Genome MH - Sex Determination Processes PMC - PMC13712589 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:34 PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:34 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PGENETICS-D-26-00470 [pii] AID - 10.1371/journal.pgen.1012333 [doi] PST - epublish SO - PLoS Genet. 2026 Oct 27;22(10):e1012333. doi: 10.1371/journal.pgen.1012333. eCollection 2026 Oct. PMID- 42945191 OWN - NLM STAT- Publisher LR - 20261027 IS - 2159-8290 (Electronic) IS - 2159-8274 (Linking) DP - 2026 Oct 27 TI - Dual-Targeted Agent Improves Advanced BTC Survival. PG - OF1 LID - 10.1158/2159-8290.CD-NW2026-0101 [doi] AB - Interim results from the phase 3 HARMONi-GI1 trial demonstrate the first-in-class PD-1xVEGF bispecific antibody ivonescimab, combined with gemcitabine and cisplatin, significantly improved overall survival compared with durvalumab plus the same chemotherapy backbone in advanced biliary tract cancer. The regimen may represent a new standard of care for first-line advanced biliary tract cancer, though questions regarding patient selection and applicability beyond Chinese populations remain. CI - (c)2026 American Association for Cancer Research. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Cancer Discov JT - Cancer discovery JID - 101561693 SB - IM EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 13:34 PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:34 [entrez] AID - 789687 [pii] AID - 10.1158/2159-8290.CD-NW2026-0101 [doi] PST - aheadofprint SO - Cancer Discov. 2026 Oct 27:OF1. doi: 10.1158/2159-8290.CD-NW2026-0101. PMID- 42945189 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Efficacy evaluation of the cervical posterior "keyhole technique" in the treatment of cervical radiculopathy: A retrospective case series. PG - e0359633 LID - 10.1371/journal.pone.0359633 [doi] LID - e0359633 AB - STUDY DESIGN: Retrospective clinical study. OBJECTIVE: To evaluate the efficacy of endoscopic posterior keyhole decompression for cervical radiculopathy and to investigate the correlation between decompression parameters and clinical outcomes. SUMMARY OF BACKGROUND DATA: Cervical radiculopathy traditionally requires anterior cervical discectomy and fusion (ACDF) or open posterior decompression. Although ACDF is the gold standard, it is associated with adjacent segment degeneration and implant-related complications. Minimally invasive posterior keyhole techniques aim to reduce surgical trauma; however, the optimal extent of decompression required for symptomatic relief remains unclear. METHODS: Forty patients with single-level cervical radiculopathy treated via unilateral endoscopic posterior keyhole decompression (strictly limited to targeted foraminotomy and partial medial facetectomy) (2021-2024) were reviewed. Clinical outcomes were assessed using the Visual Analog Scale (VAS) and Neck Disability Index (NDI) preoperatively, at discharge, and at 6 months postoperatively. Decompression parameters were quantified via postoperative CT. Statistical analysis included paired t-tests and Pearson correlation. RESULTS: All 40 procedures were successful. Specifically, 25 patients underwent isolated foraminotomy, and 15 received foraminotomy combined with discectomy, without any progression to interlaminar decompression or laminectomy. The mean operative time was 70.4 +/- 24.7 minutes, and the mean blood loss was 11.7 +/- 10.9 ml. Significant improvements in clinical scores were observed at all postoperative time points (P < 0.05). Statistical analysis revealed that neither the concurrent execution of a discectomy nor the diameter of the bony decompression window was significantly associated with the degree of postoperative symptom improvement. CONCLUSIONS: This study demonstrates that endoscopic posterior keyhole decompression is a safe and feasible technique that provides favorable short-term clinical outcomes with minimal surgical trauma. The findings strongly suggest that clinical efficacy depends on targeted neural decompression (determined by intraoperative nerve root mobility) rather than the maximal removal of disc tissue or extensive laminoforaminotomy. CI - Copyright: (c) 2026 Tang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Tang, Ruohui AU - Tang R AD - Department of Orthopedic, Kunming First People's Hospital, Affiliated Calmette Hospital of Kunming Medical University, Kunming, Yunnan, China. FAU - Yin, Jin AU - Yin J AD - Department of Orthopedic, Kunming First People's Hospital, Affiliated Calmette Hospital of Kunming Medical University, Kunming, Yunnan, China. FAU - Zhang, Zhixiang AU - Zhang Z AD - Department of Orthopedic, Kunming First People's Hospital, Affiliated Calmette Hospital of Kunming Medical University, Kunming, Yunnan, China. FAU - Chen, Zichao AU - Chen Z AD - Department of Orthopedic, Kunming First People's Hospital, Affiliated Calmette Hospital of Kunming Medical University, Kunming, Yunnan, China. FAU - Li, Yixin AU - Li Y AD - Department of Orthopedic, Kunming First People's Hospital, Affiliated Calmette Hospital of Kunming Medical University, Kunming, Yunnan, China. FAU - Zhou, Zhaowen AU - Zhou Z AUID- ORCID: 0009-0001-4386-1332 AD - Department of Orthopedic, Kunming First People's Hospital, Affiliated Calmette Hospital of Kunming Medical University, Kunming, Yunnan, China. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Humans MH - *Radiculopathy/surgery MH - Retrospective Studies MH - Female MH - *Decompression, Surgical/methods MH - *Cervical Vertebrae/surgery MH - Middle Aged MH - Male MH - Treatment Outcome MH - Endoscopy/methods MH - Foraminotomy/methods MH - Aged MH - Adult MH - Diskectomy/methods PMC - PMC13712521 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:34 PHST- 2026/06/18 00:00 [received] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:34 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PONE-D-26-28826 [pii] AID - 10.1371/journal.pone.0359633 [doi] PST - epublish SO - PLoS One. 2026 Oct 27;21(10):e0359633. doi: 10.1371/journal.pone.0359633. eCollection 2026. PMID- 42945178 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 21 IP - 10 DP - 2026 TI - Cross-platform comparison of diabetic foot ulcer transcriptomes identifies partially concordant disease-associated signals. PG - e0356328 LID - 10.1371/journal.pone.0356328 [doi] LID - e0356328 AB - The extent to which diabetic foot ulcer transcriptomic findings agree across cohorts remains uncertain. We compared an RNA sequencing cohort of 13 ulcer and eight diabetic foot skin samples with a microarray cohort of six ulcer and three diabetic foot skin samples. Single-cell data from 26 participants were analyzed after aggregating gene counts within each patient and cell type. A further cohort of 10 ulcer and 11 healthy foot-skin samples provided a descriptive directional comparison. Healing-status comparisons were exploratory. The primary analysis selected 4,704 differentially expressed gene identifiers; 2,943 were represented among the 17,502 gene symbols shared with the microarray cohort. Of these, 1,254 had concordant directions and nominal microarray P < 0.05, including 1,028 with microarray false discovery rate < 0.05. Direction agreement was 70.2%, and overall effect-rank correlation was modest (Spearman rho = 0.29). Five Hallmark gene sets met the cross-cohort enrichment criteria. More specific functional analysis of the stricter supported set highlighted cornified-envelope formation, keratinization and inflammatory-cell migration. In the additional cohort, 611 of 823 represented genes from this set agreed in direction, although its comparator differed. Twelve keratinocyte genes met the cell-type differential-expression threshold; this finding does not establish cell-type exclusivity. Healing-status results were sensitive to covariate adjustment. These data support partial concordance in ulcer-associated epithelial, inflammatory and metabolic expression patterns. Limited cohort coverage, comparator differences and unresolved confounding preclude a universal disease signature, healing predictor or causal interpretation. CI - Copyright: (c) 2026 Huang, Li. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. FAU - Huang, Shiqin AU - Huang S AD - Department of Endocrinology, Zhangjiajie People's Hospital, Zhangjiajie, Hunan, China. AD - Department of Gastroenterology, Shenzhen Xinhua Hospital, Shenzhen, Guangdong, China. FAU - Li, Yukun AU - Li Y AUID- ORCID: 0009-0003-3089-2919 AD - Department of Endocrinology, Zhangjiajie People's Hospital, Zhangjiajie, Hunan, China. LA - eng PT - Comparative Study PT - Journal Article DEP - 20261027 PL - United States TA - PLoS One JT - PloS one JID - 101285081 SB - IM MH - Humans MH - *Diabetic Foot/genetics/pathology/metabolism MH - *Transcriptome MH - Male MH - Female MH - Gene Expression Profiling MH - Wound Healing/genetics MH - Middle Aged MH - Cohort Studies MH - Skin/metabolism/pathology MH - Aged PMC - PMC13712624 COIS- The authors have declared that no competing interests exist. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:33 PHST- 2026/08/02 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:33 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - PONE-D-26-38707 [pii] AID - 10.1371/journal.pone.0356328 [doi] PST - epublish SO - PLoS One. 2026 Oct 27;21(10):e0356328. doi: 10.1371/journal.pone.0356328. eCollection 2026. PMID- 42945149 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2561-326X (Electronic) IS - 2561-326X (Linking) VI - 10 DP - 2026 Oct 27 TI - Refining a Multicomponent Family-Based Health Education Package to Reduce Screen Time Among School-Aged Children: Qualitative Formative Evaluation With Parents and Caregivers. PG - e106521 LID - 10.2196/106521 [doi] LID - e106521 AB - BACKGROUND: Excessive screen time among school-aged children is a growing concern for child health and family routines. A previous systematic review of children aged 6 to 14 years found that excessive screen time is common in this age group, highlighting the need for feasible intervention strategies that can be implemented in daily family routines. Although many health education materials communicate the harms of excessive screen use, families often need practical support to translate risk messages into household rules, parent-child communication, alternative activities, and changes to the home environment. OBJECTIVE: This study aimed to conduct a qualitative formative evaluation of a preliminary multicomponent family-based health education package designed to support healthier screen-use practices among school-aged children, with a primary focus on reducing excessive recreational screen time and associated family routines, and to identify refinements needed before pilot implementation. METHODS: Semistructured online video interviews were conducted with 10 parents or primary caregivers of school-aged children aged 6 to 12 years from October to December 2024. The package included caregiver education for parents and grandparents, child-facing materials such as a picture book and posters, home-use reminder cues, and home-environment guidance through an online self-assessment tool that generated tailored household action suggestions. The tasks assigned to the tool were limited to these functions. Participants reviewed the package components and commented on clarity, acceptability, perceived usefulness, feasibility, fit with family routines, and suggestions for refinement. Data were analyzed using directed content analysis and thematic analysis, informed by the Theoretical Framework of Acceptability (TFA) and health communication material-evaluation dimensions. RESULTS: Four themes were generated. First, the package was generally regarded as acceptable to the participating caregivers, but comprehension did not always translate into self-reported immediate recall during interviews. Second, concrete, scenario-based messages were described as more meaningful than abstract risk information. Third, caregivers wanted action guidance rather than additional risk information. Fourth, caregivers preferred practical, age-adapted, and home-embedded tools that could be repeatedly encountered in daily routines. CONCLUSIONS: Among this selected group of caregivers, the preliminary multicomponent family-based screen-time education package was generally perceived as understandable and acceptable, but participant-informed refinements are needed before pilot implementation. The findings suggest that the package should move beyond general risk communication toward a family action toolkit that combines caregiver guidance, child-facing materials, concrete consequence chains, parent-child negotiation supports, replacement activity lists, and home environmental cues. CI - (c) Mo Zhou, Yujie Yan, Siwei Sun, Hui Yin. Originally published in JMIR Formative Research (https://formative.jmir.org). FAU - Zhou, Mo AU - Zhou M AUID- ORCID: 0009-0009-1516-3631 AD - The Second People's Hospital of Shenzhen and First Affiliated Hospital of Shenzhen University, Shenzhen, China. FAU - Yan, Yujie AU - Yan Y AUID- ORCID: 0000-0002-8749-268X AD - China Japan Friendship Hospital, Beijing, China. AD - Peking University China-Japan Friendship School of Clinical Medicine, Beijing, China. FAU - Sun, Siwei AU - Sun S AUID- ORCID: 0009-0008-4107-8177 AD - Peking University Sixth Hospital, Peking University Institute of Mental Health, NHC Key Laboratory of Mental Health (Peking University), National Clinical Research Center for Mental Disorders (Peking University Sixth Hospital), Beijing, China. FAU - Yin, Hui AU - Yin H AUID- ORCID: 0000-0003-4013-1331 AD - School of Public Health, Peking University, No. 38 Xueyuan Road, Haidian District, Beijing, 100191, China, 86 1082805853. AD - Institute for Global Health, Peking University, Beijing, China. LA - eng PT - Journal Article DEP - 20261027 PL - Canada TA - JMIR Form Res JT - JMIR formative research JID - 101726394 SB - IM MH - Humans MH - Child MH - Male MH - Female MH - Qualitative Research MH - *Screen Time MH - *Health Education/methods/standards MH - *Parents/education/psychology MH - *Caregivers/education/psychology MH - Adult MH - Parent-Child Relations PMC - PMC13712257 OTO - NOTNLM OT - caregivers OT - digital media OT - family-based intervention OT - formative evaluation OT - health education OT - qualitative research OT - school-aged children OT - screen time EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 13:14 PHST- 2026/07/08 00:00 [received] PHST- 2026/08/31 00:00 [revised] PHST- 2026/09/12 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:14 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - v10i1e106521 [pii] AID - 106521 [pii] AID - 10.2196/106521 [doi] PST - epublish SO - JMIR Form Res. 2026 Oct 27;10:e106521. doi: 10.2196/106521. PMID- 42945138 OWN - NLM STAT- Publisher LR - 20261027 IS - 2373-9878 (Electronic) IS - 2373-9878 (Linking) DP - 2026 Oct 27 TI - Linking Circulating Metal Levels to Cytokine Signatures in Total Joint Arthroplasty Patients. LID - 10.1021/acsbiomaterials.6c01420 [doi] AB - Arthroplasty may significantly improve the quality of life; however, wear and corrosion of metallic biomaterials can lead to the release of metal ions and particles, contributing to immune responses. Circulating metals may influence cytokine production, but systemic biomarkers of implant-related inflammation remain elusive. This study aimed to quantify systemic metal levels and cytokines to identify potential immunological signatures in patients exposed to arthroplasty metals. Peripheral blood samples from 150 patients scheduled for either primary or revision arthroplasty were analyzed. Whole blood multi-metal levels were quantified by inductively coupled plasma mass spectrometry. Plasma levels of 47 cytokines were quantified using a bead-based multiplex immunoassay. Cytokine patterns were examined via clustering and correlated with metal levels, adjusting for age, sex, prosthetic joint infection, lymphocyte count, C-reactive protein levels, and season. Blood levels of cobalt, chromium, titanium, niobium, and zirconium were significantly elevated in patients with arthroplasty implants, compared to arthroplasty-naive controls. Selective correlations were observed, notably cobalt with IL-8 and multiple metals with IL-15. Cytokine clustering identified four distinct modules representing systemic immune activity. Multivariable linear regression modeling revealed associations of metal exposure with IL-1RA, IL-8, IL-15, and the proinflammatory cluster after adjusting for age, sex, periprosthetic joint infection status, inflammation markers, and season. This study shows that arthroprosthetic metal exposure is associated with systemic elevation of selective cytokines. Blood-based cytokine profiles may serve as biomarkers for monitoring implant-related immune activation, providing insights into mechanisms of systemic inflammation, yet must be interpreted within the context of multifactorial influences. CI - (c) 2026 The Author(s). Published by American Chemical Society. FAU - Tavakina, Anastasia AU - Tavakina A AD - Center for Orthopaedics, Trauma Surgery and Rehabilitation Medicine, University Medicine Greifswald, 17475Greifswald, Germany. AD - Institute of Immunology, University Medicine Greifswald, 17475Greifswald, Germany. FAU - Kasabji, Feras AU - Kasabji F AD - Center for Orthopaedics, Trauma Surgery and Rehabilitation Medicine, University Medicine Greifswald, 17475Greifswald, Germany. FAU - Rakow, Anastasia AU - Rakow A AUID- ORCID: 0000-0001-6171-8292 AD - Center for Orthopaedics, Trauma Surgery and Rehabilitation Medicine, University Medicine Greifswald, 17475Greifswald, Germany. FAU - Hosseini, Behnaz Alsadat AU - Hosseini BA AD - Center for Orthopaedics, Trauma Surgery and Rehabilitation Medicine, University Medicine Greifswald, 17475Greifswald, Germany. AD - Institute of Immunology, University Medicine Greifswald, 17475Greifswald, Germany. FAU - Sendler, Matthias AU - Sendler M AD - Department of Medicine A, University Medicine Greifswald, 17475Greifswald, Germany. FAU - Huesker, Katrin AU - Huesker K AD - Immunology Department, Institute for Medical Diagnostics (IMD), 12247Berlin, Germany. FAU - Volker, Uwe AU - Volker U AUID- ORCID: 0000-0002-5689-3448 AD - Department Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, 17475Greifswald, Germany. FAU - Wassilew, Georgi I AU - Wassilew GI AD - Center for Orthopaedics, Trauma Surgery and Rehabilitation Medicine, University Medicine Greifswald, 17475Greifswald, Germany. FAU - Broker, Barbara AU - Broker B AD - Institute of Immunology, University Medicine Greifswald, 17475Greifswald, Germany. FAU - Ameling, Sabine AU - Ameling S AUID- ORCID: 0000-0002-3095-8362 AD - Department Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, 17475Greifswald, Germany. FAU - Schoon, Janosch AU - Schoon J AUID- ORCID: 0000-0003-2337-0795 AD - Center for Orthopaedics, Trauma Surgery and Rehabilitation Medicine, University Medicine Greifswald, 17475Greifswald, Germany. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - ACS Biomater Sci Eng JT - ACS biomaterials science & engineering JID - 101654670 SB - IM OTO - NOTNLM OT - arthroplasty OT - cytokines OT - metal exposure OT - metal ions OT - metallic biomaterials OT - systemic inflammation EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 13:03 PHST- 2026/07/31 00:00 [received] PHST- 2026/08/14 00:00 [revised] PHST- 2026/08/17 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:03 [entrez] AID - 5459307 [pii] AID - 10.1021/acsbiomaterials.6c01420 [doi] PST - aheadofprint SO - ACS Biomater Sci Eng. 2026 Oct 27. doi: 10.1021/acsbiomaterials.6c01420. PMID- 42945134 OWN - NLM STAT- Publisher LR - 20261027 IS - 2380-0194 (Electronic) IS - 2380-0186 (Linking) DP - 2026 Oct 26 TI - Creating a Solid Posterolateral Fusion for a Minimally Invasive Lumbar Spine Surgery: A Technique Article. LID - 10.1097/BSD.0000000000002198 [doi] AB - STUDY DESIGN: A technique article. OBJECTIVE: Minimally invasive lumbar spine fusion (MIS fusion) creates a biomechanical construct for bony healing while minimizing soft tissue injury. We offer a novel technique for augmenting fusion within the posterolateral elements of the lumbar spine, which include the facet joint and transverse processes. BACKGROUND: Minimally invasive spinal fusions are designed to limit muscle dissection. We have outlined a process to perform a posterolateral fusion utilizing novel techniques for bone graft placement and creation of a fusion bed. MATERIALS AND METHODS: The article summarizes the surgical technique for MIS fusion in the posterolateral elements. Three cases of a minimally invasive transforaminal lumbar interbody fusion, posterolateral fusion/decompression, and a posterolateral fusion in the setting of a lateral lumbar interbody fusion are presented. The associated 1-year postoperative computed tomography scans and patient-reported outcomes are shown. RESULTS: The 3 cases of MIS posterolateral lumbar fusion demonstrate fusion mass formation. Patient-reported outcomes also show positive results compared with baseline values for the 3 illustrative cases. CONCLUSIONS: Posterolateral fusion using MIS techniques can be challenging for surgeons. Our novel technique of bone graft placement with creation of a fusion bed shows positive clinical results. CI - Copyright (c) 2026 Wolters Kluwer Health, LLC. All rights reserved. FAU - James, Benjamin AU - James B AD - Department of Orthopaedic Surgery at the Donald and Barbara Zucker School of Medicine Zucker School of Medicine at Hofstra at North Shore University Hospital and Long Island Jewish Medical Center, 300 Community Drive, Manhasset, NY. FAU - Emeson, Ebube AU - Emeson E FAU - Virk, Sohrab AU - Virk S LA - eng PT - Journal Article DEP - 20261026 PL - United States TA - Clin Spine Surg JT - Clinical spine surgery JID - 101675083 SB - IM OTO - NOTNLM OT - fusion OT - minimally invasive surgery OT - posterolateral fusion COIS- S.V. receives consultant fees from Isto Biologics. Isto Biologics provided facilities and supplies for this manuscript. The remaining authors declare no conflict of interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 13:03 PHST- 2025/08/09 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:03 [entrez] AID - 01933606-990000000-00861 [pii] AID - 10.1097/BSD.0000000000002198 [doi] PST - aheadofprint SO - Clin Spine Surg. 2026 Oct 26. doi: 10.1097/BSD.0000000000002198. PMID- 42945131 OWN - NLM STAT- Publisher LR - 20261027 IS - 2380-0194 (Electronic) IS - 2380-0186 (Linking) DP - 2026 Oct 19 TI - Efficacy and Safety of Ultrasound-Guided Versus Fluoroscopy-Guided Percutaneous Vertebroplasty in Osteoporotic Vertebral Compression Fractures: A Systematic Review and Meta-Analysis. LID - 10.1097/BSD.0000000000002194 [doi] AB - STUDY DESIGN: Systematic review and meta-analysis. OBJECTIVE: To compare the efficacy and safety of ultrasound-guided versus fluoroscopy-guided PVP in the treatment of OVCFs. SUMMARY OF BACKGROUND DATA: Fluoroscopy-guided percutaneous vertebroplasty (PVP) is a common treatment for osteoporotic vertebral compression fractures (OVCFs). Nonetheless, the radiation-related risks and the drawbacks of imaging have prompted the investigation of ultrasound-guided PVP. METHODS: A systematic review and meta-analysis comparing ultrasound-guided and fluoroscopy-guided PVP was conducted. The outcomes included were pain relief, functional improvement, operation duration, radiation dose, cement distribution, and complication rates. RESULTS: Ultrasound-guided PVP had comparable pain reduction and functional outcomes to fluoroscopy-guided procedures. Radiation exposure and operation time were significantly reduced in the ultrasound-guided group. The use of real-time visualization of soft tissues improved the accuracy of needle placement and minimized cement leakage. There was no significant difference in complication rates between the groups. Recent comparative studies of advanced fluoroscopy-guided techniques, such as robot-assisted and patient-specific guide-assisted procedures, have resulted in further improvements in procedural efficiency and cement leakage rates, with comparable pain relief and functional outcomes. CONCLUSIONS: PVP using ultrasound guidance is a safe and effective alternative for fluoroscopy-guided PVP, with lower radiation exposure and better procedural safety. These findings require further trials in large-scale studies to verify them and improve clinical practice. CI - Copyright (c) 2026 Wolters Kluwer Health, LLC. All rights reserved. FAU - Yan, Ting AU - Yan T AD - Department of Ultrasound Medicine, Honghui Hospital, Xi'an Jiaotong University, Xi 'an, Shaanxi, China. FAU - Wang, Xiangyang AU - Wang X AD - Department of Orthopedics (Spine Surgery), Xi'an Hospital of Traditional Chinese Medicine, Xi'an, China. FAU - Liu, Bo AU - Liu B AD - Department of Orthopedics (Spine Surgery), Xi'an Hospital of Traditional Chinese Medicine, Xi'an, China. FAU - Guan, Haiying AU - Guan H AD - Department of Orthopedics (Spine Surgery), Xi'an Hospital of Traditional Chinese Medicine, Xi'an, China. LA - eng PT - Journal Article DEP - 20261019 PL - United States TA - Clin Spine Surg JT - Clinical spine surgery JID - 101675083 SB - IM OTO - NOTNLM OT - fluoroscopy OT - minimally invasive surgical procedures OT - spinal fractures OT - ultrasonography OT - vertebroplasty COIS- The authors declare no conflict of interest.​ EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 13:03 PHST- 2025/10/20 00:00 [received] PHST- 2026/08/25 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 13:03 [entrez] AID - 01933606-990000000-00860 [pii] AID - 10.1097/BSD.0000000000002194 [doi] PST - aheadofprint SO - Clin Spine Surg. 2026 Oct 19. doi: 10.1097/BSD.0000000000002194. PMID- 42945125 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1465-2099 (Electronic) IS - 0022-1317 (Print) IS - 0022-1317 (Linking) VI - 107 IP - 10 DP - 2026 Oct TI - Crimean-Congo haemorrhagic fever virus S-segment mRNA 5' UTR functions as an internal ribosome entry site-like element in an N protein-dependent manner. LID - 10.1099/jgv.0.002359 [doi] LID - 002359 AB - The Crimean-Congo haemorrhagic fever virus nucleocapsid (N) protein contains structurally distinct stalk and head domains. The mRNA encoding N protein (NP) possesses a 55-nt 5' UTR that folds into a stable hairpin structure. We previously demonstrated that the NP preferentially promotes translation of its own mRNA by simultaneously binding the 5' UTR hairpin via its stalk domain and the translation initiation factor eIF4A via its head domain. This coordinated interaction facilitates selective ribosome recruitment to the 5' UTR and enhances translation of the downstream ORF. In this study, we further show that the S-segment 5' UTR functions as an internal ribosome entry site (IRES)-like element when inserted between two ORFs, promoting downstream translation in an NP-dependent manner. Expression of a dual-reporter transcript containing the S-segment 5' UTR in Hazara virus-infected cells inhibited viral replication by competing for NP binding. In contrast, randomization of the 5' UTR abolished NP binding, IRES-like activity and antiviral effects. Unlike the S segment, the L- and M-segment 5' UTRs do not form the requisite hairpin structure, fail to bind NP and cannot support NP-mediated translation. These findings suggest that structurally similar elements within host mRNAs may also be selectively translated during infection. FAU - Ren, Songyang AU - Ren S AD - College of Veterinary Medicine, Western University of Health Sciences, Pomona, California, USA. FAU - Mir, Ethan AU - Mir E AD - Department of Microbiology, Immunology and Molecular Genetics, UCLA, Los Angeles, California, USA. FAU - Le, Hoangvi AU - Le H AD - College of Veterinary Medicine, Western University of Health Sciences, Pomona, California, USA. FAU - Ali, Saima AU - Ali S AD - College of Veterinary Medicine, Western University of Health Sciences, Pomona, California, USA. FAU - Mir, Sheema AU - Mir S AD - College of Veterinary Medicine, Western University of Health Sciences, Pomona, California, USA. FAU - Mir, Mohammad AU - Mir M AD - College of Veterinary Medicine, Western University of Health Sciences, Pomona, California, USA. LA - eng PT - Journal Article PL - England TA - J Gen Virol JT - The Journal of general virology JID - 0077340 RN - 0 (5' Untranslated Regions) RN - 0 (Internal Ribosome Entry Sites) RN - 0 (RNA, Viral) RN - 0 (RNA, Messenger) RN - 0 (Nucleocapsid Proteins) SB - IM MH - *5' Untranslated Regions MH - *Internal Ribosome Entry Sites/genetics MH - *RNA, Viral/genetics/metabolism MH - *Hemorrhagic Fever Virus, Crimean-Congo/genetics/physiology/metabolism MH - *RNA, Messenger/genetics/metabolism MH - *Nucleocapsid Proteins/genetics/metabolism MH - Animals MH - Protein Biosynthesis MH - Humans MH - Virus Replication MH - Cell Line MH - Open Reading Frames MH - Ribosomes/metabolism MH - Nucleic Acid Conformation PMC - PMC13712975 OTO - NOTNLM OT - Crimean-Congo haemorrhagic fever virus OT - internal ribosome entry site (IRES) OT - nucleocapsid protein OT - translation COIS- The authors declare that there are no conflicts of interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 12:54 PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:54 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 002359 [pii] AID - 10.1099/jgv.0.002359 [doi] PST - ppublish SO - J Gen Virol. 2026 Oct;107(10):002359. doi: 10.1099/jgv.0.002359. PMID- 42945119 OWN - NLM STAT- Publisher LR - 20261029 IS - 2198-3844 (Electronic) IS - 2198-3844 (Linking) DP - 2026 Oct 27 TI - Potassium-Impurity-Modulated Self-Trapped Excitons and Energy Transfer in Phase-Separated, Mn(4+)-Doped Li(2)HfF(6). LID - e78338 [pii] LID - 10.1002/advs.78338 [doi] AB - Self-trapped excitons (STEs) play an important role in the luminescence of halide materials, yet their formation and manipulation in complex fluorides remain largely underexplored. Here, Mn(4+)-doped Li(2)HfF(6) phosphors were synthesized by room-temperature coprecipitation to investigate impurity-modulated STEs and their role in Mn(4+) sensitization. High-resolution neutron powder diffraction reveals impurity-associated phase separation into two closely related Li(2)HfF(6) lattices. Photoluminescence and cathodoluminescence measurements resolve two broad host-emission components: STE1, assigned to intrinsic Li(2)HfF(6), and redshifted STE2, associated with a K-containing lattice. Under high-energy cathodoluminescence excitation, enhanced Mn(4+) red emission correlates with STE2 emission but not with STE1 emission, supporting a selective STE2 --> Mn(4+) energy-transfer pathway. Density functional theory DeltaSCF (self-consistent field) calculations yield distinct relaxed STE1 and STE2 configurations, confirming the K-induced redshift of the STE emission. These results identify K-associated STE2 as a key sensitizing state for Mn(4+) luminescence, establishing impurity modulation as an effective strategy for tuning excitonic emission and host-to-activator energy transfer in complex fluoride phosphors. CI - (c) 2026 The Author(s). Advanced Science published by Wiley-VCH GmbH. FAU - Adhikari, Menuka AU - Adhikari M AD - Department of Chemistry, Physics, and Materials Science, Fayetteville State University, Fayetteville, North Carolina, USA. FAU - Murray, Thomas AU - Murray T AD - Department of Chemistry, Physics, and Materials Science, Fayetteville State University, Fayetteville, North Carolina, USA. FAU - Lewis, Cameron J AU - Lewis CJ AD - Department of Chemistry, Physics, and Materials Science, Fayetteville State University, Fayetteville, North Carolina, USA. FAU - Wu, Jett AU - Wu J AD - Department of Chemistry, Physics, and Materials Science, Fayetteville State University, Fayetteville, North Carolina, USA. FAU - Adhikari, Hari AU - Adhikari H AUID- ORCID: 0009-0006-2686-0756 AD - Center for Nanoscale Materials, Argonne National Laboratory, Lemont, Illinois, USA. FAU - Li, Cheng AU - Li C AUID- ORCID: 0000-0002-6546-6413 AD - Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA. FAU - Wen, Jianguo AU - Wen J AUID- ORCID: 0000-0002-3755-0044 AD - Center for Nanoscale Materials, Argonne National Laboratory, Lemont, Illinois, USA. FAU - Wu, Hui AU - Wu H AUID- ORCID: 0000-0003-0296-5204 AD - NIST Center for Neutron Research, National Institute of Standards and Technology, Gaithersburg, Maryland, USA. FAU - Luo, Zhiping AU - Luo Z AUID- ORCID: 0000-0002-8264-6424 AD - Department of Chemistry, Physics, and Materials Science, Fayetteville State University, Fayetteville, North Carolina, USA. LA - eng GR - DMR 1827731/National Science Foundation/ GR - OIA2430295/National Science Foundation/ GR - DMR1626376/National Science Foundation/ GR - NIST/United States Government/ GR - FY2027/North Carolina General Assembly/ GR - DE-AC02-06CH11357/Department of Energy/ GR - IPTS-30170.1/Oak Ridge National Laboratory/ PT - Journal Article DEP - 20261027 PL - Germany TA - Adv Sci (Weinh) JT - Advanced science (Weinheim, Baden-Wurttemberg, Germany) JID - 101664569 SB - IM PMC - PMC13712481 OTO - NOTNLM OT - Li2HfF6 OT - density functional theory (DFT) OT - doping OT - fluoride OT - impurity OT - luminescence OT - self-trapped exciton COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 PMCR- 2026/10/27 CRDT- 2026/10/27 12:53 PHST- 2026/09/18 00:00 [revised] PHST- 2026/08/03 00:00 [received] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:53 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e78338 [pii] AID - ADVS78338 [pii] AID - 10.1002/advs.78338 [doi] PST - aheadofprint SO - Adv Sci (Weinh). 2026 Oct 27:e78338. doi: 10.1002/advs.78338. PMID- 42945117 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261029 IS - 1864-564X (Electronic) IS - 1864-5631 (Print) IS - 1864-5631 (Linking) VI - 19 IP - 20 DP - 2026 Oct 28 TI - Tuning the Selectivity of Glycerol Electrooxidation via Catalyst Composition and Operating Conditions. LID - e71189 [pii] LID - 10.1002/cssc.71189 [doi] AB - The electrooxidation of glycerol presents a promising route for upgrading a biodiesel byproduct into high-value chemicals while lowering the energy cost of electrochemical hydrogen production, but its mechanistic understanding remains incomplete. This work investigates the influence of temperature and catalyst composition on glycerol electrooxidation using three distinct electrocatalysts: first-row transition-metal NiCo oxide, noble metal AuIn alloy, and hybrid PdNi alloy. Electrochemical, in situ UV-vis reflectance and in situ Raman measurements reveal catalyst-specific oxidation mechanisms: an oxyhydroxide-mediated path for NiCo, an adsorbed hydroxide-mediated path for AuIn which suffers from inhibition, and a dual mechanism for PdNi. Oxyhydroxide mechanisms favor formic acid, whereas adsorbed hydroxide pathways selectively promote C(3) products such as glyceric acid and dihydroxyacetone. Flow cell electrolysis across a range of temperatures and flow rates relevant to industrial application shows that elevated temperature enhances reaction rates and mitigates catalyst inhibition, a relevant effect for the hybrid catalyst. These findings provide key mechanistic insights and practical guidelines for optimizing continuous-flow glycerol electrooxidation systems. CI - (c) 2026 The Author(s). ChemSusChem published by Wiley-VCH GmbH. FAU - Molera, Marti AU - Molera M AUID- ORCID: 0000-0002-7210-981X AD - Sustainable Electrochemical Processes (SEP), Departament de Ciencia de Materials i Quimica Fisica & Institut de Quimica Teorica i Computacional (IQTCUB), Facultat de Quimica, Universitat de Barcelona (UB), Barcelona, Spain. AD - Departament d'Enginyeria Electronica i Biomedica, Facultat de Fisica, Universitat de Barcelona (UB), Barcelona, Spain. FAU - Golovanova, Viktoria AU - Golovanova V AUID- ORCID: 0000-0002-4008-0733 AD - Institut de Ciencies Fotoniques (ICFO), Barcelona Institute of Science and Technology, Castelldefels, Spain. FAU - Garcia de Arquer, F Pelayo AU - Garcia de Arquer FP AUID- ORCID: 0000-0003-2422-6234 AD - Institut de Ciencies Fotoniques (ICFO), Barcelona Institute of Science and Technology, Castelldefels, Spain. FAU - Cuesta, Angel AU - Cuesta A AUID- ORCID: 0000-0003-4243-1848 AD - Advanced Centre for Energy and Sustainability (ACES), School of Natural and Computing Sciences, University of Aberdeen, Aberdeen, UK. AD - Instituto de Quimica Fisica Blas Cabrera, Madrid, Spain. FAU - Andreu, Teresa AU - Andreu T AUID- ORCID: 0000-0002-2804-4545 AD - Sustainable Electrochemical Processes (SEP), Departament de Ciencia de Materials i Quimica Fisica & Institut de Quimica Teorica i Computacional (IQTCUB), Facultat de Quimica, Universitat de Barcelona (UB), Barcelona, Spain. LA - eng GR - PID2022-138491OB-C33PID2022-138127NA-I00CEX2021-001202-MCEX2024-001490-S/Ministerio de Ciencia e Innovacion/ GR - 101077243101115204/European Commission/ GR - 2024 FI-1 00421/Agencia de Gestio d'Ajuts Universitaris i de Recerca/ PT - Journal Article PL - Germany TA - ChemSusChem JT - ChemSusChem JID - 101319536 SB - IM PMC - PMC13712567 OTO - NOTNLM OT - UV-vis reflectance OT - flow-cell electrolysis OT - glycerol oxidation OT - temperature COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 12:52 PHST- 2026/10/03 00:00 [revised] PHST- 2026/05/11 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:52 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e71189 [pii] AID - CSSC71189 [pii] AID - 10.1002/cssc.71189 [doi] PST - ppublish SO - ChemSusChem. 2026 Oct 28;19(20):e71189. doi: 10.1002/cssc.71189. PMID- 42945115 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261027 IS - 1864-564X (Electronic) IS - 1864-5631 (Linking) VI - 19 IP - 20 DP - 2026 Oct 28 TI - Controlling Hydrogenation-Ammonolysis Competition in the Selective Reductive Amination of Biomass-Derived Furfural Over Pt-Ni Catalysts. LID - e71165 [pii] LID - 10.1002/cssc.71165 [doi] AB - Understanding the factors controlling selectivity in the reductive amination of biomass-derived furfural remains a major challenge for the rational design of sustainable catalytic processes. Herein, selective reductive amination of biomass-derived furfural to furfurylamine (FAM) was achieved over TiO(2)-supported Pt-Ni nanoparticles derived from carbonyl clusters as well-defined bimetallic precursors. Tuning the Pt/Ni ratio enabled the identification of Pt(2)Ni(4)/TiO(2) as the most efficient catalyst, reaching 92% furfurylamine selectivity and a productivity of 92 molFAM molmetal(-1) h(-1) under mild conditions. This study integrates kinetic analysis with density functional theory (DFT) to provide qualitative support for Ni-to-Pt electron redistribution and suggests that the bimetallic interface facilitated furfural activation, in agreement with the experimentally observed enhancement in catalytic performance. DFT calculations reveal that Ni incorporation induces a significant electronic redistribution, promoting electron transfer toward Pt surface sites and substantially lowering the activation barrier of the hydrogenation step to 0.37 eV. Furthermore, we establish that FAM selectivity emerges from a dynamic interplay where surface ammonia coverage dictates the competition between difurfurylimine hydrogenation and ammonolysis. We also identify the non-innocent role of methanol via the formation of methoxyimine intermediates, which bypass traditional reaction pathways under hydrogen-limited conditions. These findings demonstrate that selective reductive amination is governed by coverage-dependent intermediate equilibria on the bimetallic surface rather than by metal identity alone. This work provides general design principles for controlling the hydrogenation-ammonolysis competition in the sustainable upgrading of renewable platform molecules. CI - (c) 2026 Wiley-VCH GmbH. FAU - Liuzzi, Francesca AU - Liuzzi F AD - Department of Industrial Chemistry, C3-Centre for Chemical Catalysis, University of Bologna, Bologna, Italy. FAU - Corradini, Matteo AU - Corradini M AUID- ORCID: 0009-0004-2027-4225 AD - Department of Industrial Chemistry, C3-Centre for Chemical Catalysis, University of Bologna, Bologna, Italy. FAU - Cesari, Cristiana AU - Cesari C AUID- ORCID: 0000-0003-2595-2078 AD - Department of Industrial Chemistry, C3-Centre for Chemical Catalysis, University of Bologna, Bologna, Italy. FAU - Allegri, Alessandro AU - Allegri A AUID- ORCID: 0000-0003-4721-5167 AD - Department of Industrial Chemistry, C3-Centre for Chemical Catalysis, University of Bologna, Bologna, Italy. FAU - Di Renzo, Francesco AU - Di Renzo F AUID- ORCID: 0000-0002-0515-8128 AD - ICGM, Universite de Montpellier-CNRS-ENSCM, Montpellier, France. FAU - Hounfodji, Jean Wilfried AU - Hounfodji JW AD - L2CM UMR 7053, Universite de Lorraine, CNRS, Nancy, France. FAU - Badawi, Michael AU - Badawi M AUID- ORCID: 0000-0002-3504-4180 AD - L2CM UMR 7053, Universite de Lorraine, CNRS, Nancy, France. FAU - Wojcieszak, Robert AU - Wojcieszak R AD - L2CM UMR 7053, Universite de Lorraine, CNRS, Nancy, France. FAU - Zacchini, Stefano AU - Zacchini S AUID- ORCID: 0000-0003-0739-0518 AD - Department of Industrial Chemistry, C3-Centre for Chemical Catalysis, University of Bologna, Bologna, Italy. FAU - Dimitratos, Nikolaos AU - Dimitratos N AUID- ORCID: 0000-0002-6620-4335 AD - Department of Industrial Chemistry, C3-Centre for Chemical Catalysis, University of Bologna, Bologna, Italy. FAU - Bernardi, Luca AU - Bernardi L AUID- ORCID: 0000-0002-7840-3200 AD - Department of Industrial Chemistry, C3-Centre for Chemical Catalysis, University of Bologna, Bologna, Italy. FAU - Albonetti, Stefania AU - Albonetti S AUID- ORCID: 0000-0002-2371-3228 AD - Department of Industrial Chemistry, C3-Centre for Chemical Catalysis, University of Bologna, Bologna, Italy. LA - eng GR - Code P202282F9N/Italian PRIN 2022 PNRR/ GR - 2023-20090/RER-6/Italian regional project FSE+2021-2027/ PT - Journal Article PL - Germany TA - ChemSusChem JT - ChemSusChem JID - 101319536 SB - IM OTO - NOTNLM OT - ammonolysis OT - biomass valorization OT - furfural OT - heterogeneous catalysis OT - metal carbonyl clusters OT - reductive amination EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:43 PHST- 2026/08/27 00:00 [revised] PHST- 2026/08/27 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:43 [entrez] AID - e71165 [pii] AID - 10.1002/cssc.71165 [doi] PST - ppublish SO - ChemSusChem. 2026 Oct 28;19(20):e71165. doi: 10.1002/cssc.71165. PMID- 42945110 OWN - NLM STAT- Publisher LR - 20261028 IS - 1941-0506 (Electronic) IS - 1077-2626 (Linking) VI - PP DP - 2026 Oct 27 TI - The Impact of Point-Cloud Rendering on User Perceptions of Human- and AI-Controlled Avatar Embodiment. LID - 10.1109/TVCG.2026.3747541 [doi] AB - Avatars for social VR are increasingly driven by imperfect tracking or AI-generated motion, where mismatches between appearance fidelity and behavioral realism can harm user perception. We investigate whether a simulated point-cloud rendering (PCR) can improve social perception and increase tolerance to motion artifacts compared to standard shaded mesh rendering. We present a real-time VR avatar system that supports (i) a streaming conversational loop, (ii) human-controlled embodiment via hybrid tracking using a head-mounted display for upper body and an external camera for lower body and root motion, and (iii) AI-controlled embodiment via context-aware selection from an offline-generated animation library. We evaluate a 2x2x2 within-subjects study crossing rendering style (PCR vs. mesh) and control source (human vs. AI) across two sequential scenarios: a conversation-focused desk interaction and a movement-focused guided exercise. We measure social presence, human-likeness and affinity, and appearance-behavior plausibility, and collect post-study rendering-style preference rankings. Our results show that PCR significantly improves perceived human-likeness and plausibility, with particularly strong benefits for AI-controlled avatars. Crucially, PCR successfully closed the plausibility gap between human and AI avatars during high-movement interactions, acting as a perceptual buffer against motion artifacts. While social presence was driven by the interaction scenario rather than rendering style, post-study rankings revealed a trade-off between perceptual coherence and visual comfort. FAU - Song, Tianyu AU - Song T FAU - Gursoy, Baran AU - Gursoy B FAU - Pabst, Felix AU - Pabst F FAU - Eck, Ulrich AU - Eck U FAU - Navab, Nassir AU - Navab N LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - IEEE Trans Vis Comput Graph JT - IEEE transactions on visualization and computer graphics JID - 9891704 SB - IM EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:43 PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 12:43 [entrez] AID - 10.1109/TVCG.2026.3747541 [doi] PST - aheadofprint SO - IEEE Trans Vis Comput Graph. 2026 Oct 27;PP. doi: 10.1109/TVCG.2026.3747541. PMID- 42945107 OWN - NLM STAT- Publisher LR - 20261028 IS - 1941-0506 (Electronic) IS - 1077-2626 (Linking) VI - PP DP - 2026 Oct 27 TI - Investigating Depth-Based Target Expansion for Gaze Selection with Dwell Activation in Virtual Environments. LID - 10.1109/TVCG.2026.3747517 [doi] AB - While dwell-based selection enables interaction with little physical effort, it is prone to (eye) gaze jitter. Target expansion methods mitigate this problem by increasing the effective selectable target area in 2D environments. Yet depth-related factors may limit their effectiveness in 3D virtual environments due to stereo-deficiencies. In this paper, we investigate how depth affects target expansion methods for dwell-based gaze selection, and propose DEPTH-BASED expansion, a method that adapts target expansion based on depth. We conducted two user studies to evaluate our proposed method with an ISO 9241:411 multidirectional selection study and a 3D selection study in a complex environment. Our results show that while the effectiveness of 2D target expansion methods decreases significantly with increasing depth, our proposed method consistently improved user performance and experience. Our findings provide design insights for effective dwell-based selection of expanding targets in 3D virtual environments. FAU - Turkmen, Rumeysa AU - Turkmen R FAU - Rasoulifar, Zahra AU - Rasoulifar Z FAU - Mutasim, Aunnoy K AU - Mutasim AK FAU - Batmaz, Anil Ufuk AU - Batmaz AU LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - IEEE Trans Vis Comput Graph JT - IEEE transactions on visualization and computer graphics JID - 9891704 SB - IM EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:43 PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 12:43 [entrez] AID - 10.1109/TVCG.2026.3747517 [doi] PST - aheadofprint SO - IEEE Trans Vis Comput Graph. 2026 Oct 27;PP. doi: 10.1109/TVCG.2026.3747517. PMID- 42945103 OWN - NLM STAT- Publisher LR - 20261028 IS - 1941-0506 (Electronic) IS - 1077-2626 (Linking) VI - PP DP - 2026 Oct 27 TI - Effects of Field-of-View Restriction and Peripheral Teleportation on Path Integration during Virtual Locomotion. LID - 10.1109/TVCG.2026.3747523 [doi] AB - Most software-based cybersickness mitigation techniques, such as field-of-view (FOV) restrictors and rest frames, alter visual content and peripheral optical flow, often negatively affecting various locomotion quality factors. Path integration, a critical component of spatial updating, relies on optical flow during virtual locomotion and may therefore be particularly affected by these techniques. Despite a substantial body of work evaluating cybersickness mitigation methods, relatively few studies have examined their effects on spatial updating or path integration. Moreover, prior studies on spatial updating have not yielded conclusive results, despite theoretical predictions from the perception literature. In this work, we conducted a preregistered experiment with 48 participants to compare the effects of two mitigation techniques, the dynamic FOV restrictor and peripheral teleportation, on path integration, relative to an unrestricted control condition. By minimizing potential confounding factors identified in previous work, we found that participants made significantly larger homing errors with the FOV restrictor than in the control condition, whereas performance with peripheral teleportation did not differ significantly from either of the other conditions. These findings provide direct evidence that FOV restriction impairs path integration, while the impact of peripheral teleportation remains inconclusive. Our results further highlight the trade-off between mitigation effectiveness and spatial updating, providing VR designers with deeper context for selecting mitigation techniques. FAU - Nie, Tongyu AU - Nie T FAU - Huang, Yuxuan AU - Huang Y FAU - Cantory, Ville AU - Cantory V FAU - Zhang, Danhua AU - Zhang D FAU - Rosenberg, Evan Suma AU - Rosenberg ES LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - IEEE Trans Vis Comput Graph JT - IEEE transactions on visualization and computer graphics JID - 9891704 SB - IM EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:43 PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 12:43 [entrez] AID - 10.1109/TVCG.2026.3747523 [doi] PST - aheadofprint SO - IEEE Trans Vis Comput Graph. 2026 Oct 27;PP. doi: 10.1109/TVCG.2026.3747523. PMID- 42945101 OWN - NLM STAT- Publisher LR - 20261028 IS - 1941-0506 (Electronic) IS - 1077-2626 (Linking) VI - PP DP - 2026 Oct 27 TI - From Motion to Meaning: A Systematic Review of Motion Tracking in XR-based Experimental Psychology. LID - 10.1109/TVCG.2026.3747488 [doi] AB - Immersive technologies such as Virtual Reality (VR), Augmented Reality (AR), and Mixed Reality (MR) are being increasingly used in experimental psychology because they enable controlled environments while simultaneously producing high-frequency motiontracking data from headsets, controllers, and body trackers. These motion traces provide continuous behavioral signals that may reflect affective, behavioral, and cognitive processes during human-human, human-computer, and human-virtual human interactions. Despite the growing use of motion measures in XR (Extended Reality) experiments, there is limited synthesis of how such data are collected, transformed, analyzed, and interpreted. This paper presents a systematic literature review of studies collected from the ACM Digital Library, IEEE Xplore, PsycINFO, PubMed, and Web of Science that use motion-tracking data from XR devices in experimental psychology, focusing on affect, behavior, cognition, or their intersections. The review examines the devices used, the body parts tracked, and the data formats employed in these studies. More importantly, it analyzes how raw motion-tracking data streams are transformed into kinematic or trajectory-level features and the analytical methods used to link them to psychological constructs. Our findings highlight substantial variability in device configurations and motion data extraction methods, but we observe a common pattern of using descriptive statistical measures to summarize motion patterns or applying machine learning models to learn from processed motion features. Based on these observations, we outline practical recommendations for selecting tracking setups, collecting and validating motion data, and designing analysis pipelines in experimental psychological studies. This review aims to support researchers seeking to incorporate motion-tracking data into XR-based psychological experiments and to promote more consistent and interpretable use of motion measures in immersive research. FAU - Mannan, M M Rushadul AU - Mannan MMR FAU - Miller, Mark Roman AU - Miller MR LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - IEEE Trans Vis Comput Graph JT - IEEE transactions on visualization and computer graphics JID - 9891704 SB - IM EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:43 PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 12:43 [entrez] AID - 10.1109/TVCG.2026.3747488 [doi] PST - aheadofprint SO - IEEE Trans Vis Comput Graph. 2026 Oct 27;PP. doi: 10.1109/TVCG.2026.3747488. PMID- 42945100 OWN - NLM STAT- Publisher LR - 20261028 IS - 1941-0506 (Electronic) IS - 1077-2626 (Linking) VI - PP DP - 2026 Oct 27 TI - Collaborative Immersive Analytics: A Systematic Review. LID - 10.1109/TVCG.2026.3747518 [doi] AB - Collaborative Immersive Analytics (CIA) has emerged as a promising approach for supporting collective sense-making of data using immersive technologies. Over the past decade, a growing number of systems have been proposed, yet the design space and collaborative practices in CIA remain fragmented across the literature. This paper presents a systematic review of CIA research published between 2015 and 2025. Following PRISMA guidelines, 19 empirical studies were analysed across six dimensions: time-space configuration, analytical tasks, system-centred symmetry, user-centred symmetry, collaboration dynamics, and data characteristics and domain applications. The analysis results show that CIA systems predominantly supported synchronous collaboration and shared exploratory analysis coordinated through natural communication mechanisms such as verbal communication and deictic gestures. However, many systems were evaluated using datasets selected primarily to demonstrate system capabilities rather than real-world analytical applications. Several design considerations are outlined for future CIA research. FAU - Nguyen, Huyen AU - Nguyen H LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - IEEE Trans Vis Comput Graph JT - IEEE transactions on visualization and computer graphics JID - 9891704 SB - IM EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:43 PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 12:43 [entrez] AID - 10.1109/TVCG.2026.3747518 [doi] PST - aheadofprint SO - IEEE Trans Vis Comput Graph. 2026 Oct 27;PP. doi: 10.1109/TVCG.2026.3747518. PMID- 42945081 OWN - NLM STAT- Publisher LR - 20261028 IS - 1941-0506 (Electronic) IS - 1077-2626 (Linking) VI - PP DP - 2026 Oct 27 TI - Contextual Blocking Entities: Transformation of Guardian Boundaries in Virtual Reality. LID - 10.1109/TVCG.2026.3747447 [doi] AB - Conventional Virtual Reality (VR) guardian systems communicate physical boundaries through grid-based overlays that effectively signal spatial limits, but disrupt narrative coherence and presence. To address this safety-presence trade-off, we propose Contextual Blocking Entities (CBEs), a boundary design approach that transforms guardian boundaries into narrative-integrated virtual objects by embedding spatial constraints within the internal logic of the virtual world. We define CBE design through three dimensions, Function Match, which signals impassability through visual form, Context Consistency, which determines how naturally the object belongs to the world, and Constraint Type, which determines how the boundary geometry engages spatial processing. To operationalize these dimensions, we first conducted a formative study classifying 80 candidate virtual objects, then carried out a controlled within-subject user study using a 2x2x2 factorial design to compare CBE conditions against a grid baseline across presence, spatial awareness, and boundary compliance. All CBE conditions produced significantly higher presence than the grid baseline, while four conditions showed no significant difference from the baseline in boundary violations and all conditions maintained comparable Mean Return Time. The three dimensions played asymmetric roles across boundary interaction. Function Match supported approach deterrence and conservative spatial estimation, Context Consistency supported post-violation correction, and Constraint Type shaped actual movement range more strongly than explicit spatial judgment. These findings show that the safety-presence trade-off depends on how spatial constraints are expressed, yielding practical guidelines for CBE selection and placement in room-scale VR. FAU - Jang, Sohyun AU - Jang S FAU - Kang, Seongjun AU - Kang S FAU - Um, Juwon AU - Um J FAU - Park, Jeongju AU - Park J FAU - Kim, SeungJun AU - Kim S LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - IEEE Trans Vis Comput Graph JT - IEEE transactions on visualization and computer graphics JID - 9891704 SB - IM EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:43 PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 12:43 [entrez] AID - 10.1109/TVCG.2026.3747447 [doi] PST - aheadofprint SO - IEEE Trans Vis Comput Graph. 2026 Oct 27;PP. doi: 10.1109/TVCG.2026.3747447. PMID- 42945055 OWN - NLM STAT- Publisher LR - 20261027 IS - 2040-3372 (Electronic) IS - 2040-3364 (Linking) DP - 2026 Oct 27 TI - Acid-base etching of vinasse-derived precursors for high-performance hard carbon anodes in sodium-ion batteries. LID - 10.1039/d6nr00510a [doi] AB - Vinasse, an abundantly produced biomass waste, is valorized as low-value products such as animal feed. Its conversion into hard carbon (HC) materials represents a promising route for high-value utilization. However, vinasse-derived HC without targeted compositional regulation fails to meet electrochemical performance benchmarks for practical sodium-ion battery (SIB) applications. To address this challenge, we report a two-step acid-base treatment strategy for vinasse precursor modification, enabling the fabrication of high-performance HC anodes. Sulfuric acid treatment selectively removes hemicellulose and transition metal impurities, while sodium hydroxide treatment eliminates silica and modulates the lignin structure, achieving synergistic chemical reconstruction of the precursor. The resultant HC exhibits a hierarchically optimized microstructure with abundant closed pores, high sp(2) carbon content, and the absence of ash-related crystalline phases. As a result, the optimized sample delivers a reversible capacity of 349.4 mAh g(-1) at 0.1C with an initial coulombic efficiency (ICE) of 88.7%. Moreover, it demonstrates exceptional cycling stability, retaining 83.6% of its initial capacity after 2000 cycles at 5C, in stark contrast to the untreated sample, which retains 68.3%. This work establishes a scalable and facile route for converting low-cost biomass precursors into high-performance HC anodes, offering a viable pathway toward sustainable and economically competitive SIBs. FAU - Xu, Wenjing AU - Xu W AD - Shanghai Institute of Ceramics, Chinese Academy of Science, Shanghai 200050, China. AD - Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Science, Beijing 100049, China. FAU - Gao, YuSha AU - Gao Y AD - Shanghai Institute of Ceramics, Chinese Academy of Science, Shanghai 200050, China. AD - Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Science, Beijing 100049, China. FAU - Tian, Zhangliu AU - Tian Z AD - Shanghai Institute of Ceramics, Chinese Academy of Science, Shanghai 200050, China. FAU - Zhou, Ce AU - Zhou C AD - Beijing Nadi Technology Co., Ltd., Beijing, 102600, China. FAU - Tan, Yue AU - Tan Y AD - Lenovo (Beijing) Co., Ltd., No. 10 XiBeiWang East Road, Haidian District, Beijing 100094, China. FAU - Wu, Le AU - Wu L AD - Lenovo (Beijing) Co., Ltd., No. 10 XiBeiWang East Road, Haidian District, Beijing 100094, China. FAU - Guan, Yue AU - Guan Y AD - Lenovo (Beijing) Co., Ltd., No. 10 XiBeiWang East Road, Haidian District, Beijing 100094, China. FAU - Zhao, Wei AU - Zhao W AD - Shanghai Institute of Ceramics, Chinese Academy of Science, Shanghai 200050, China. FAU - Wang, Meng AU - Wang M AD - Shanghai Institute of Ceramics, Chinese Academy of Science, Shanghai 200050, China. FAU - Huang, Fuqiang AU - Huang F AUID- ORCID: 0000-0003-0526-5473 AD - Key Laboratory of Intelligent Creation for Extreme Energy Materials of Ministry of Education, School of Materials Science and Engineering and Zhang jiang Institute for Advanced Study, Shanghai Jiao Tong University, Shanghai 200240, China. AD - School of Materials Science and Engineering and Zhang jiang Institute for Advanced Study, Shanghai Jiao Tong University, Shanghai, 200240, China. AD - Inner Mongolia Research Institute, Shanghai Jiao Tong University, Hohhot, 010010, China. LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Nanoscale JT - Nanoscale JID - 101525249 SB - IM EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:24 PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:24 [entrez] AID - 10.1039/d6nr00510a [doi] PST - aheadofprint SO - Nanoscale. 2026 Oct 27. doi: 10.1039/d6nr00510a. PMID- 42945047 OWN - NLM STAT- Publisher LR - 20261027 IS - 1933-7205 (Electronic) IS - 1933-7191 (Linking) DP - 2026 Oct 27 TI - Evaluation and Disposition of Potential Candidates for Uterine Transplantation: Experience From a Large Referral Center. LID - 10.1007/s43032-026-02251-z [doi] AB - OBJECTIVE: Uterine transplantation (UTx) is an emerging surgical treatment for absolute uterine factor infertility. Given its complexity, careful patient selection and counseling are essential. We aimed to characterize patients expressing interest in UTx and identify common reasons for exclusion at a large referral center. METHODS: We conducted a retrospective cohort study of individuals who self-referred for UTx and completed a standardized intake survey between April 2022 and April 2025 at a large academic center. Demographic, reproductive, medical, and psychosocial data were collected. Applications were reviewed by the transplant team, with additional phone screening when needed. The primary outcome was reason for exclusion from UTx candidacy. RESULTS: During the 3-year study period, 371 intake surveys were received; 3 (0.8%) were incomplete. Causes of infertility included prior hysterectomy (79.6%), congenitally absent uterus (16.3%), and other etiologies (4.1%). Among 368 complete applications, 96.0% (353/368) were excluded. Reasons included age >/= 40 years, BMI >/= 30 kg/m(2), tobacco or illicit substance use, medical contraindications, severe mental health conditions, reproductive contraindications, self-withdrawal, and inability to comply with study requirements. The most common exclusions were medical contraindications (34%), age (18%), and BMI (17%). CONCLUSIONS: Fewer than 2% of interested applicants ultimately qualify for UTx. While many criteria are nonmodifiable, addressing modifiable factors may improve candidacy and support informed counseling. TRIAL REGISTRATION: Clinical trial registration number- NCT05263076. CI - (c) 2026. The Author(s). FAU - Ciomperlik, Hailie N AU - Ciomperlik HN AD - Department of Obstetrics and Gynecology, Baylor College of Medicine and Texas Children's Fetal Center, Houston, TX, USA. FAU - Alexander, Megan V AU - Alexander MV AD - Department of Obstetrics and Gynecology, Baylor College of Medicine and Texas Children's Fetal Center, Houston, TX, USA. FAU - Reding, Anita AU - Reding A AD - Department of Surgery, Baylor College of Medicine and Texas Children's Fetal Center, Houston, TX, USA. FAU - Galvan, Thao AU - Galvan T AD - Department of Surgery, Baylor College of Medicine and Texas Children's Fetal Center, Houston, TX, USA. FAU - Johnson, Rebecca M AU - Johnson RM AD - Department of Obstetrics and Gynecology, Baylor College of Medicine and Texas Children's Fetal Center, Houston, TX, USA. FAU - Goss, John A AU - Goss JA AD - Department of Surgery, Baylor College of Medicine and Texas Children's Fetal Center, Houston, TX, USA. FAU - Detti, Laura AU - Detti L AD - Department of Obstetrics and Gynecology, Baylor College of Medicine and Texas Children's Fetal Center, Houston, TX, USA. FAU - Belfort, Michael A AU - Belfort MA AD - Department of Obstetrics and Gynecology, Baylor College of Medicine and Texas Children's Fetal Center, Houston, TX, USA. FAU - Munoz, Jessian L AU - Munoz JL AUID- ORCID: 0000-0003-0431-5280 AD - Department of Obstetrics and Gynecology, Baylor College of Medicine and Texas Children's Fetal Center, Houston, TX, USA. Jessian.munoz@bcm.edu. AD - Department of OB/GYN, Texas Children's Hospital, 6621 Fannin Street, Houston, TX, 77030, USA. Jessian.munoz@bcm.edu. LA - eng SI - ClinicalTrials.gov/NCT05263076 PT - Journal Article DEP - 20261027 PL - United States TA - Reprod Sci JT - Reproductive sciences (Thousand Oaks, Calif.) JID - 101291249 SB - IM OTO - NOTNLM OT - Candidate OT - Exclusion OT - Uterine transplant COIS- Declarations. Ethics Approval and Consent to Participate: This study was approved by the Institutional Review Board at Baylor College of Medicine. Completion of the survey was considered implied informed consent. Consent for Publication: No identifiable individual data are included in this manuscript; therefore, consent for publication was not required. Competing Interests: The authors report no conflicts of interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:24 PHST- 2026/02/22 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:24 [entrez] AID - 10.1007/s43032-026-02251-z [pii] AID - 10.1007/s43032-026-02251-z [doi] PST - aheadofprint SO - Reprod Sci. 2026 Oct 27. doi: 10.1007/s43032-026-02251-z. PMID- 42945043 OWN - NLM STAT- Publisher LR - 20261027 IS - 1867-1314 (Electronic) IS - 1867-1306 (Linking) DP - 2026 Oct 27 TI - Characterization of Fusaricidin Producing Paenibacillus kribbensis and Development of Active Polymer Film Based on its Extract for Anti-Oomycete Coating Applications. LID - 10.1007/s12602-026-11215-3 [doi] AB - Zingiber officinale Roscoe. is an economically important spice crop severely affected by the rhizome rot caused by the necrotrophic oomycete pathogen Pythium myriotylum, resulting in substantial yield losses. Zingiber zerumbet, a wild relative of cultivated ginger, has innate resistance to several phytopathogens and hence may serve as a source of beneficial endophytic bacteria for the disease management. This study explores the biocontrol potential of endophytic bacteria Paenibacillus kribbensis ZzP, isolated from wild ginger, against P. myriotylum. The selected ZzP isolate was observed to cause 81.25 +/- 0.32% inhibition of P. myriotylum as revealed by the dual culture assay result along with suppression of tissue necrosis in ginger caused by the pathogen as evidenced in the bright field microscopic studies. Upon LC-MS/MS based profiling, crude extract of P. kribbensis ZzP has revealed to have the presence of putative fusaricidin-type cyclic lipopeptide. The whole-genome analysis has also identified the ZzP to have NRPS-associated biosynthetic gene cluster (BGC) supporting its fusaricidin biosynthetic potential. The metabolite from ZzP was further incorporated into polyvinyl alcohol (PVA) based biocomposite film which was demonstrated to be effective against P. myriotylum in chilli fruit when applied in the form of dip coating. The active biocomposite film has shown to have a water contact angle of 52.03 degrees and the XRD, FTIR and SEM results further demonstrated successful incorporation of the ZzP metabolites in the film matrix. The biodegradability analysis of the film has also shown it to have a rapid weight loss within 21 days. This was further supported by the FTIR results showing peak shifts as indication of degradation and destabilisation of the polymer molecules. To the best of our knowledge, this study represents the first report of an endophytic fusaricidin-producing P. kribbensis strain exhibiting antagonistic activity against P. myriotylum, which highlight its potential for sustainable crop protection applications. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Manu, Smruthy AU - Manu S AD - School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, 686560, India. FAU - Jose, Annu AU - Jose A AD - School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, 686560, India. FAU - Varghese, Riya Rachel AU - Varghese RR AD - School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, 686560, India. FAU - Surendrababu, Swathy Puthanvila AU - Surendrababu SP AD - Centre of Excellence (CoE-KIST), Kerala Institute of Science, Technology and Innovation, Mahatma Gandhi University, Kottayam, Kerala, 686560, India. FAU - Sreejith, Arjun AU - Sreejith A AD - School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, 686560, India. FAU - Alex, Riya AU - Alex R AD - School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, 686560, India. FAU - George, Julit AU - George J AD - School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, 686560, India. FAU - James, Arsha AU - James A AD - School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, 686560, India. FAU - Mathew, Jyothis AU - Mathew J AD - School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, 686560, India. FAU - Jayachandran, K AU - Jayachandran K AD - School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, 686560, India. FAU - Radhakrishnan, E K AU - Radhakrishnan EK AUID- ORCID: 0000-0003-2688-701X AD - School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, 686560, India. radhakrishnanek@mgu.ac.in. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Probiotics Antimicrob Proteins JT - Probiotics and antimicrobial proteins JID - 101484100 SB - IM OTO - NOTNLM OT - Paenibacillus kribbensis OT - Pythium myriotylum OT - Biocomposite film OT - Biocontrol OT - Fusaricidin OT - Ginger OT - Rhizome protection COIS- Declarations. Authorship Confirmation: All authors hereby confirm consent to the submission of this manuscript, acknowledge authorship of the work, and accept full responsibility for the accuracy and integrity of the content presented. Author Agreement Statement: We, the undersigned authors, hereby certify that this manuscript is an original work, has not been previously published, and is not under consideration for publication by any other journal or publisher. We confirm that all listed authors have reviewed and approved the manuscript and that no individual who meets the criteria for authorship has been omitted. We further affirm that the sequence of authors presented in the manuscript has been mutually agreed upon by all authors. We acknowledge that the Corresponding Author serves as the primary point of contact throughout the editorial and publication process and is responsible for keeping all co-authors informed regarding manuscript progress, revision submissions, and the final approval of proofs. Competing interests: The authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:23 PHST- 2026/06/26 00:00 [received] PHST- 2026/09/10 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:23 [entrez] AID - 10.1007/s12602-026-11215-3 [pii] AID - 10.1007/s12602-026-11215-3 [doi] PST - aheadofprint SO - Probiotics Antimicrob Proteins. 2026 Oct 27. doi: 10.1007/s12602-026-11215-3. PMID- 42945042 OWN - NLM STAT- Publisher LR - 20261027 IS - 1867-1314 (Electronic) IS - 1867-1306 (Linking) DP - 2026 Oct 27 TI - Multi-Criterion In Vitro Profiling of Probiotic Strains for Preliminary Gut-Skin-Axis-Oriented Candidate Prioritization. LID - 10.1007/s12602-026-11269-3 [doi] AB - The gut-skin axis is a promising target for oral probiotics, yet strain choice is often empirical, rather than aligned to defined, skin-relevant functional readouts. Here, we performed a predefined multi-criterion in vitro screening of lactobacilli and bifidobacteria to prioritize candidate strains for gut-skin-axis-oriented development. The screening combined four complementary readouts: antagonistic activity against selected skin-associated pathobionts, targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) profiling of metabolites in cell-free supernatants (CFS), a simple surface-binding assay based on binding to polystyrene, and transepithelial electrical resistance (TEER) monitoring in Caco-2 intestinal epithelial monolayers during early monolayer maturation. Antimicrobial activity was strongly strain dependent and most pronounced against Staphylococcus aureus and Cutibacterium spp., with Lacticaseibacillus rhamnosus SCN01 and Limosilactobacillus fermentum LF160 showing the broadest inhibition profiles. Replicated metabolomics revealed distinct, reproducible extracellular signatures: lactate dominated across lactobacilli, B. breve SCN03 showed the clearest acetate increase, and L. fermentum LF160 displayed a coordinated arginine-to-ornithine/citrulline shift with high succinate. Nicotinic acid (vitamin B3) accumulated most in Lactobacillus acidophilus LA120 and B. breve SCN03, whereas pyridoxal (vitamin B6) and GABA were most enriched in L. plantarum SCN02; in the shortlisted strains, selected biogenic amines remained low in the replicated targeted panel. Functionally, L. rhamnosus SCN01 produced the strongest sustained TEER increase without detectable changes in cellular metabolic activity. Collectively, these data support a transparent, hypothesis-generating framework for preliminary in vitro prioritization of oral probiotic candidates with complementary antimicrobial, metabolic, surface-binding and epithelial-resistance-associated profiles. Further mechanistic, community-based, skin-cell, preclinical and clinical studies will be required to determine whether these in vitro profiles translate into gut-skin-axis-relevant benefits. CI - (c) 2026. The Author(s). FAU - Aleksandrzak-Piekarczyk, Tamara AU - Aleksandrzak-Piekarczyk T AUID- ORCID: 0000-0002-4725-760X AD - Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland. tamara@ibb.waw.pl. FAU - Tymoszewska, Aleksandra AU - Tymoszewska A AD - Microbiota Lab, Department of Pharmaceutical Microbiology and Bioanalysis, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1B, 02-097, Warsaw, Poland. FAU - Radkiewicz, Mariusz AU - Radkiewicz M AD - Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland. FAU - Szczepankowska, Agnieszka K AU - Szczepankowska AK AD - Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland. FAU - Samborowska, Emilia AU - Samborowska E AD - Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland. FAU - Koryszewska-Baginska, Anna AU - Koryszewska-Baginska A AD - Department of Medical Biology, Medical University of Warsaw, Litewska 14/16, 00-575, Warsaw, Poland. FAU - Cukrowska, Bozena AU - Cukrowska B AD - Department of Research and Development, Nordic Biotic Ltd., Zlota Street 59, 00-120, Warsaw, Poland. LA - eng GR - NB222/2025/Nordic Biotic Ltd./ GR - NB225/2025/Propharma Ltd./ PT - Journal Article DEP - 20261027 PL - United States TA - Probiotics Antimicrob Proteins JT - Probiotics and antimicrobial proteins JID - 101484100 SB - IM OTO - NOTNLM OT - Antimicrobial activity OT - Bifidobacteria OT - Gut-skin axis OT - Lactobacilli OT - Metabolomics OT - Probiotics OT - Skin health OT - TEER COIS- Declarations. Competing interests: B.C. is Chief of the Research and Development Department at Nordic Biotic Ltd. Several strains included in this study belong to branded collections associated with Nordic Biotic Ltd. and/or Propharma Ltd., and the study was partially financed by Nordic Biotic Ltd. and Propharma Ltd. The top-ranked candidate strains include strains from these collections. B.C. contributed to the manuscript as a co-author through Writing - review & editing. The academic authors were responsible for experimental design, data generation, data analysis, interpretation and manuscript drafting. Except for the funding support and B.C.'s declared co-author contribution, the funders did not influence data analysis, interpretation, the decision to submit, or the conclusions of the manuscript. Nordic Biotic Ltd. and/or Propharma Ltd. own or commercialize selected tested strains, as indicated in Table 1. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:23 PHST- 2026/03/09 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:23 [entrez] AID - 10.1007/s12602-026-11269-3 [pii] AID - 10.1007/s12602-026-11269-3 [doi] PST - aheadofprint SO - Probiotics Antimicrob Proteins. 2026 Oct 27. doi: 10.1007/s12602-026-11269-3. PMID- 42945036 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2198-4034 (Electronic) IS - 2198-4034 (Linking) VI - 12 IP - 1 DP - 2026 Oct 27 TI - Patient-specific subperiosteal implants for oral and maxillofacial rehabilitation: a narrative review of current evidence, clinical decision-making, and unresolved questions. LID - 10.1186/s40729-026-00730-1 [doi] LID - 57 AB - PURPOSE: Contemporary patient-specific subperiosteal implants (PSSIs) have re-emerged as digitally planned reconstructive devices for severe jaw atrophy, previous treatment failure, and complex maxillofacial defects. This narrative review critically evaluates current evidence, clinical decision-making, and unresolved questions across indications, design, treatment workflows, outcomes, complications, and maintenance. METHODS: Evidence identification drew on the structured search developed for the companion Delphi study. PubMed/MEDLINE, Embase, and CENTRAL were searched from 2000 to 19 December 2025, supplemented by trial registries, grey literature, and reference-list screening. Targeted PubMed searches and reference screening were updated through 31 August 2026. Clinical, biomechanical, technical, and translational evidence was synthesized narratively. RESULTS: Modern PSSIs integrate three-dimensional imaging, virtual prosthetically driven planning, CAD/CAM design, additive manufacturing, and planned skeletal fixation. Available evidence supports feasibility and high short-term survival in selected patients, but longer-term cohorts and procedure-specific analyses indicate that implant retention may overestimate durable clinical success. Soft-tissue recession, dehiscence, mucositis, and framework exposure are the dominant biological complications and appear central to late failure. Applications are expanding beyond full-arch atrophic jaws to sectional, congenital, salvage, and oncologic reconstruction despite marked heterogeneity in indications, device design, loading protocols, and outcome definitions. Direct comparative evidence remains scarce. CONCLUSIONS: PSSIs should be considered a selected prosthetically driven reconstructive option rather than a broadly equivalent alternative to conventional implants or established reconstruction. Future studies require standardized success and failure definitions, longer follow-up, comparative treatment-pathway evaluation, and greater integration of soft-tissue, prosthetic, patient-reported, maintenance, and cost-effectiveness outcomes. CI - (c) 2026. The Author(s). FAU - Al-Nawas, Bilal AU - Al-Nawas B AD - Department of Oral and Maxillofacial Surgery, University Medical Centre Mainz, Augustusplatz 2, 55131, Mainz, Germany. FAU - Bar, Anne-Kathrin AU - Bar AK AD - Department of Oral and Maxillofacial Surgery, University Medical Centre Mainz, Augustusplatz 2, 55131, Mainz, Germany. baeranne@uni-mainz.de. AD - Department of Oral and Maxillofacial Surgery, Federal Armed Forces Hospital, Koblenz, Germany. baeranne@uni-mainz.de. FAU - Kwon, Yong-Dae AU - Kwon YD AD - Department of Oral and Maxillofacial Surgery, College of Dentistry, Kyung Hee University, Seoul, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Germany TA - Int J Implant Dent JT - International journal of implant dentistry JID - 101676532 RN - 0 (Dental Implants) SB - IM MH - Humans MH - *Clinical Decision-Making MH - *Dental Implants MH - Computer-Aided Design MH - *Dental Implantation, Endosseous/methods MH - Periosteum/surgery MH - Dental Prosthesis Design PMC - PMC13713962 OTO - NOTNLM OT - Additive manufacturing OT - CAD/CAM OT - Implant survival OT - Maxillofacial reconstruction OT - Patient-specific implant OT - Severe jaw atrophy OT - Soft-tissue dehiscence OT - Subperiosteal implant COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 12:23 PHST- 2026/10/07 00:00 [received] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:23 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 10.1186/s40729-026-00730-1 [pii] AID - 730 [pii] AID - 10.1186/s40729-026-00730-1 [doi] PST - epublish SO - Int J Implant Dent. 2026 Oct 27;12(1):57. doi: 10.1186/s40729-026-00730-1. PMID- 42945033 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2192-4449 (Electronic) IS - 2192-4449 (Linking) VI - 15 IP - 6 DP - 2026 Oct 27 TI - Ravulizumab monotherapy for anti-CFH antibody-associated aHUS in an elderly patient: a case report highlighting individualized clinical decision-making. LID - 10.1007/s13730-026-01200-8 [doi] LID - 162 AB - Atypical hemolytic uremic syndrome (aHUS) is a life-threatening thrombotic microangiopathy caused by complement dysregulation. Although anti-complement factor H (CFH) autoantibodies are a well-recognized cause of aHUS, their occurrence in elderly patients is rare, and optimal management in this population remains unclear. We report the case of an 85-year-old man who presented with acute kidney injury, thrombocytopenia, and microangiopathic hemolytic anemia, alongside severe hypertension. ADAMTS13 activity was preserved, and no secondary causes of thrombotic microangiopathy were identified, leading to a clinical suspicion of complement-mediated thrombotic microangiopathy (TMA). At the time of clinical decision-making, anti-CFH antibody status was not yet available;serum creatinine trends during hemodialysis could not reliably indicate intrinsic renal recovery, and hematological markers, although improving, did not clearly indicate complete resolution of hemolysis. Following an individualized risk assessment regarding plasma exchange (PEX) and immunosuppressive therapy in an octogenarian, ravulizumab was selected as the initial treatment due to its etiology-independent mechanism of action. Following initiation, hematological parameters continued to improve, and renal function gradually recovered, allowing discontinuation of hemodialysis. Subsequent testing revealed elevated anti-CFH antibody levels without pathogenic complement gene variants, confirming the diagnosis of anti-CFH antibody-associated aHUS. This case highlights that, in clinical settings with diagnostic uncertainty, ravulizumab monotherapy may be considered as an individualized initial approach in exceptional cases where PEX and immunosuppression are judged unsuitable based on clearly documented patient-specific risks, and requires careful follow-up including serial anti-CFH antibody monitoring and consideration of immunosuppression if antibody titers persist or relapse risk remains high. CI - (c) 2026. The Author(s), under exclusive licence to Japanese Society of Nephrology. FAU - Yoshii, Ryuichi AU - Yoshii R AD - National Hospital Organization Kumamoto Medical Center, 1-5 Ninomaru, Kumamoto, Kumamoto, Japan. chicken_soup@icloud.com. FAU - Kajiwara, Kengo AU - Kajiwara K AD - National Hospital Organization Kumamoto Medical Center, 1-5 Ninomaru, Kumamoto, Kumamoto, Japan. FAU - Hirose, Keitaro AU - Hirose K AD - National Hospital Organization Kumamoto Medical Center, 1-5 Ninomaru, Kumamoto, Kumamoto, Japan. FAU - Hatanaka, Toshiki AU - Hatanaka T AD - National Hospital Organization Kumamoto Medical Center, 1-5 Ninomaru, Kumamoto, Kumamoto, Japan. FAU - Shimasaki, Akiko AU - Shimasaki A AD - National Hospital Organization Kumamoto Medical Center, 1-5 Ninomaru, Kumamoto, Kumamoto, Japan. FAU - Tomita, Masao AU - Tomita M AD - National Hospital Organization Kumamoto Medical Center, 1-5 Ninomaru, Kumamoto, Kumamoto, Japan. FAU - Yokoi, Hideki AU - Yokoi H AD - Kumamoto University Hospital, 1-1-1 Honjo, Kumamoto, Kumamoto, Japan. LA - eng PT - Case Reports PT - Journal Article DEP - 20261027 PL - Japan TA - CEN Case Rep JT - CEN case reports JID - 101636244 RN - 0 (Autoantibodies) RN - 0 (Antibodies, Monoclonal, Humanized) RN - 80295-65-4 (Complement Factor H) RN - A3ULP0F556 (eculizumab) RN - 0 (CFH protein, human) SB - IM MH - Humans MH - Male MH - Aged, 80 and over MH - *Atypical Hemolytic Uremic Syndrome/drug therapy/immunology/diagnosis/therapy MH - Autoantibodies/blood/immunology MH - *Antibodies, Monoclonal, Humanized/therapeutic use/administration & dosage MH - *Complement Factor H/immunology MH - Clinical Decision-Making MH - Treatment Outcome MH - Plasma Exchange/methods MH - Acute Kidney Injury PMC - PMC13713940 OTO - NOTNLM OT - Anti-CFH antibody OT - Atypical hemolytic uremic syndrome OT - Elderly OT - Hypertension OT - Ravulizumab COIS- Declarations. Conflict of interest: All the authors have declared no competing interests. Human and animal rights: This article does not contain any studies with human participants or animals performed by any of the authors. Informed consent: Informed consent was obtained from the patient in the present study. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 12:23 PHST- 2026/04/22 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:23 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 10.1007/s13730-026-01200-8 [pii] AID - 1200 [pii] AID - 10.1007/s13730-026-01200-8 [doi] PST - epublish SO - CEN Case Rep. 2026 Oct 27;15(6):162. doi: 10.1007/s13730-026-01200-8. PMID- 42945032 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1941-6636 (Electronic) IS - 1941-6628 (Print) VI - 57 IP - 1 DP - 2026 Oct 27 TI - Radioembolization for Colorectal Liver Metastases: Indications and Outcomes. LID - 10.1007/s12029-026-01617-5 [doi] LID - 244 AB - Colorectal cancer remains a leading cause of cancer-related mortality, with the liver representing the predominant site of metastatic spread. For patients with colorectal liver metastases (CRLM) who are not candidates for surgical resection, systemic chemotherapy provides disease control but rarely achieves durable hepatic responses. Radioembolization with yttrium-90 (Y-90) microspheres, delivered via the hepatic artery to exploit the preferential arterial supply of liver tumors, has emerged as an important locoregional option in this setting. This review examines the current evidence base for radioembolization in CRLM, covering patient selection, indications across lines of therapy, dosimetric advances, and clinical outcomes. Appropriate candidacy requires careful assessment of performance status, hepatic tumor burden, tumor biology, lung shunt fraction, and extrahepatic disease extent, with the strongest outcomes observed in patients selected earlier in their disease course. Randomized controlled trials support second-line and third-line use, while unselected first-line use has not demonstrated a benefit. Dosimetric advances, including multicompartment personalized dosing, radiation segmentectomy, and radiation lobectomy, are enabling ablative and bridge-to-resection strategies in appropriately selected patients. Future investigation should prioritize biomarker-driven patient selection, personalized dosimetry, and combination strategies with systemic therapy to determine whether durable hepatic disease control can translate into a survival benefit. CI - (c) 2026. The Author(s). FAU - Reddy, Sai AU - Reddy S AD - University of Chicago Pritzker School of Medicine, Chicago, IL, USA. FAU - Wu, Xiao AU - Wu X AD - Department of Vascular & Interventional Radiology, University of California San Francisco, San Francisco, CA, USA. FAU - Rodwell, Natasha AU - Rodwell N AD - Department of Vascular & Interventional Radiology, University of Chicago, 5841 S Maryland Ave, Chicago, IL, 60637, USA. FAU - Khan, Abdul AU - Khan A AD - Department of Vascular & Interventional Radiology, University of Chicago, 5841 S Maryland Ave, Chicago, IL, 60637, USA. FAU - Navuluri, Rakesh AU - Navuluri R AD - Department of Vascular & Interventional Radiology, University of Chicago, 5841 S Maryland Ave, Chicago, IL, 60637, USA. FAU - Shaffer, Virginia AU - Shaffer V AD - Department of Colon & Rectal Surgery, University of Chicago, Chicago, IL, USA. FAU - Fidelman, Nicholas AU - Fidelman N AD - Department of Vascular & Interventional Radiology, University of California San Francisco, San Francisco, CA, USA. FAU - Yu, Qian AU - Yu Q AUID- ORCID: 0000-0001-9774-9226 AD - Department of Vascular & Interventional Radiology, University of Chicago, 5841 S Maryland Ave, Chicago, IL, 60637, USA. yuqian1006@gmail.com. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - United States TA - J Gastrointest Cancer JT - Journal of gastrointestinal cancer JID - 101479627 RN - 0 (Yttrium Radioisotopes) RN - 1K8M7UR6O1 (Yttrium-90) SB - IM MH - Humans MH - *Liver Neoplasms/secondary/therapy/mortality/radiotherapy MH - *Colorectal Neoplasms/pathology/therapy/mortality MH - *Radioembolization, Therapeutic/methods MH - *Yttrium Radioisotopes/administration & dosage/therapeutic use MH - Patient Selection MH - Treatment Outcome MH - Microspheres PMC - PMC13714070 OTO - NOTNLM OT - Colorectal cancer OT - Liver metastases OT - Radioembolization OT - Selective Internal Radiation Therapy (SIRT) OT - Transarterial Radioembolization (TARE) OT - Y-90 COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 12:23 PHST- 2026/09/24 00:00 [received] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:23 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 10.1007/s12029-026-01617-5 [pii] AID - 1617 [pii] AID - 10.1007/s12029-026-01617-5 [doi] PST - epublish SO - J Gastrointest Cancer. 2026 Oct 27;57(1):244. doi: 10.1007/s12029-026-01617-5. PMID- 42945027 OWN - NLM STAT- Publisher LR - 20261027 IS - 1863-6713 (Electronic) IS - 1863-6705 (Linking) DP - 2026 Oct 27 TI - Redo minimally invasive mitral valve surgery: feasibility, practical benefits, and surgical pitfalls from a single-center experience. LID - 10.1007/s11748-026-02359-8 [doi] AB - OBJECTIVES: To describe surgical outcomes, technical considerations, and feasibility of redo minimally invasive mitral valve surgery using initial cases as a reference cohort. METHODS: A single-center, retrospective review of minimally invasive mitral valve surgery performed between January 2014 and January 2024 was conducted. Perioperative data, mid-term survival, and cardiac-related events were assessed in both initial and redo groups. RESULTS: A total of 647 patients were included: 30 in the redo group and 617 in the initial group. The redo group had higher European System for Cardiac Operative Risk Evaluation II scores (p < 0.001). In the redo group, 26 (86.7%) had undergone previous cardiac surgery via sternotomy, and 18 (60.0%) had previous mitral valve surgery. The operative time was longer in the redo group (p = 0.041), while the cross-clamping time was longer in the initial group (p = 0.019), and cardiopulmonary bypass time was comparable between groups (p = 0.95). Intubation time (p = 0.049), intensive care unit stay (p = 0.020), and in-hospital stay (p = 0.003) were longer in the redo group. However, there were no significant differences in postoperative chest tube output for 24 h (p = 0.32) or blood transfusion requirements (p = 0.087). The overall survival rates at 5 years were 70.7% (95% CI: 41.0%-87.0%) vs. 92.0% (95% CI: 88.0%-94.0%) for the redo and initial groups, respectively. Freedom from cardiac-related events did not significantly differ between groups. CONCLUSIONS: Redo minimally invasive mitral valve surgery was feasible despite higher surgical risk and longer operative times. Early and mid-term cardiac-related outcomes appeared acceptable, although overall survival was lower in redo cases. CI - (c) 2026. The Author(s), under exclusive licence to The Japanese Association for Thoracic Surgery. FAU - Nakamae, Kosuke AU - Nakamae K AUID- ORCID: 0000-0003-3848-0153 AD - Department of Cardiovascular Surgery, Chiba-Nishi General Hospital, 197-1, Kanegasaku, Matsudo-shi, Chiba, 270-2251, Japan. FAU - Nakamura, Yoshitsugu AU - Nakamura Y AUID- ORCID: 0000-0003-2510-081X AD - Department of Cardiovascular Surgery, Chiba-Nishi General Hospital, 197-1, Kanegasaku, Matsudo-shi, Chiba, 270-2251, Japan. ystgnkmr@gmail.com. FAU - Kuroda, Miho AU - Kuroda M AD - Department of Cardiovascular Surgery, Chiba-Nishi General Hospital, 197-1, Kanegasaku, Matsudo-shi, Chiba, 270-2251, Japan. FAU - Sawa, Shintaro AU - Sawa S AUID- ORCID: 0000-0003-1220-3203 AD - Department of Cardiovascular Surgery, Chiba-Nishi General Hospital, 197-1, Kanegasaku, Matsudo-shi, Chiba, 270-2251, Japan. FAU - Niitsuma, Kusumi AU - Niitsuma K AD - Department of Cardiovascular Surgery, Chiba-Nishi General Hospital, 197-1, Kanegasaku, Matsudo-shi, Chiba, 270-2251, Japan. FAU - Yasumoto, Yuto AU - Yasumoto Y AD - Department of Cardiovascular Surgery, Chiba-Nishi General Hospital, 197-1, Kanegasaku, Matsudo-shi, Chiba, 270-2251, Japan. FAU - Nakayama, Taisuke AU - Nakayama T AUID- ORCID: 0000-0002-7107-933X AD - Department of Cardiovascular Surgery, Chiba-Nishi General Hospital, 197-1, Kanegasaku, Matsudo-shi, Chiba, 270-2251, Japan. FAU - Yusa, Hiroaki AU - Yusa H AUID- ORCID: 0009-0003-5342-7825 AD - Department of Cardiovascular Surgery, Chiba-Nishi General Hospital, 197-1, Kanegasaku, Matsudo-shi, Chiba, 270-2251, Japan. FAU - Tsuruta, Ryo AU - Tsuruta R AD - Department of Cardiovascular Surgery, Chiba-Nishi General Hospital, 197-1, Kanegasaku, Matsudo-shi, Chiba, 270-2251, Japan. FAU - Ito, Yujiro AU - Ito Y AUID- ORCID: 0000-0002-1337-8390 AD - Department of Cardiovascular Surgery, Chiba-Nishi General Hospital, 197-1, Kanegasaku, Matsudo-shi, Chiba, 270-2251, Japan. LA - eng PT - Journal Article DEP - 20261027 PL - Japan TA - Gen Thorac Cardiovasc Surg JT - General thoracic and cardiovascular surgery JID - 101303952 SB - IM OTO - NOTNLM OT - Mini-thoracotomy OT - Minimally invasive cardiac surgery OT - Mitral valve surgery OT - Redo cases COIS- Declarations. Conflict of interest: None declared. Ethical approval: This retrospective study was approved by the Institutional Review Board at Chiba-Nishi General Hospital (8/29/2024, no. TGE02612-025). All patients provided written consent preoperatively. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:23 PHST- 2026/01/02 00:00 [received] PHST- 2026/07/09 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:23 [entrez] AID - 10.1007/s11748-026-02359-8 [pii] AID - 10.1007/s11748-026-02359-8 [doi] PST - aheadofprint SO - Gen Thorac Cardiovasc Surg. 2026 Oct 27. doi: 10.1007/s11748-026-02359-8. PMID- 42945013 OWN - NLM STAT- Publisher LR - 20261027 IS - 1573-4919 (Electronic) IS - 0300-8177 (Linking) DP - 2026 Oct 27 TI - Betulinic acid attenuates obesity-associated cardiac remodelling by modulating mitochondrial quality control, ferroptosis-associated signalling, and calcium-regulatory pathways. LID - 10.1007/s11010-026-05791-9 [doi] AB - Obesity-associated cardiac remodelling (OACR) is characterized by metabolic, structural, mitochondrial, oxidative, and molecular alterations that may contribute to progressive cardiac dysfunction. However, the interplay among mitochondrial quality control, ferroptosis, and calcium homeostasis in obesity-associated cardiac injury and remodelling remains incompletely understood. This study investigated whether betulinic acid (BA) attenuates obesity-induced myocardial injury by modulating these interconnected pathways. Male Sprague-Dawley rats were fed a high-fat diet (HFD) for 16 weeks to induce cardiac injury and remodelling and treated with BA (40 mg/kg, orally) for 6 weeks. Cardiac morphology, metabolic parameters, myocardial bioenergetic parameters, oxidative stress, iron homeostasis, and markers associated with mitochondrial quality control, mitochondrial dynamics, ferroptosis, and calcium regulation were assessed using biochemical, histopathological, mRNA, and protein expression analyses. Molecular docking provided hypothesis-generating predictions of potential interactions between BA and selected regulatory proteins. HFD-induced obesity was associated with alterations in mitochondrial quality-control, ferroptosis-associated, and calcium-regulatory signalling markers, including changes in the SERCA2a-CaMKIIdelta axis. BA improved metabolic abnormalities, increased myocardial ATP content and the NAD(+)/NADH ratio, and attenuated oxidative injury and iron accumulation. These effects were accompanied by increased PINK1, Parkin, MFN2, GPX4, SLC7A11, and SERCA2a expression and decreased DRP1, ACSL4, and CaMKIIdelta expression. BA attenuated OACR, accompanied by changes in mitochondrial quality-control, ferroptosis-associated, and calcium-regulatory proteins. These findings indicate that modulation of these interconnected pathways may contribute to the attenuation of OACR. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Uddandrao, V V Sathibabu AU - Uddandrao VVS AUID- ORCID: 0000-0002-1488-2302 AD - Department of Biotechnology, Karpagam Academy of Higher Education (Deemed to be University), Coimbatore, Tamil Nadu, 641021, India. sathibabu.u@gmail.com. AD - Centre for Molecular Therapeutics, Karpagam Academy of Higher Education (Deemed to be University), Coimbatore, Tamil Nadu, 641021, India. sathibabu.u@gmail.com. FAU - Kothai, R AU - Kothai R AD - Department of Pharmacology, Vinayaka Mission's College of Pharmacy, Vinayaka Mission's Research Foundation (Deemed to be University), Salem, Tamil Nadu, 636 008, India. FAU - Asokan, B R AU - Asokan BR AD - Department of Pharmacology, Trichy SRM Medical College and Research Centre (Affiliated to TN Dr. MGR Medical University, Chennai), Irungalur, Tiruchirappalli, Tamil Nadu, 621105, India. FAU - Sengottuvelu, S AU - Sengottuvelu S AD - Department of Pharmacology, Nandha College of Pharmacy, Erode, Tamil Nadu, 638052, India. FAU - Kumar, Umesh AU - Kumar U AD - Centre for Advanced Cancer Research, Chandigarh University, Gharuan, Mohali, Punjab, 140413, India. FAU - Sakthivel, Kunnathur Murugesan AU - Sakthivel KM AD - Department of Biochemistry, PSG College of Arts and Science, Coimbatore, Tamil Nadu, 641014, India. FAU - Brahmanaidu, Parim AU - Brahmanaidu P AD - Division of Preclinical Toxicology and Biochemistry, ICMR- National Institute for Pre-Clinical Research, Hyderbad, Telangana, 500101, India. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Mol Cell Biochem JT - Molecular and cellular biochemistry JID - 0364456 SB - IM OTO - NOTNLM OT - Cardiac remodelling OT - Metabolic cardiac injury OT - Metabolic dysfunction OT - Myocardial injury OT - Phytomedicine COIS- Declarations. Ethical approval: All animal experimental procedures were reviewed and approved by the Institutional Animal Ethics Committee of Sri Lakshmi Narayana Institute of Medical Sciences (IAEC Approval No: SLIMS/57/IAEC/2025-26) and were experiments conducted in accordance with guidelines issued by the Committee for Control and Supervision of Experiments on Animals (CCSEA), Government of India. Competing interests: The authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:22 PHST- 2026/08/03 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:22 [entrez] AID - 10.1007/s11010-026-05791-9 [pii] AID - 10.1007/s11010-026-05791-9 [doi] PST - aheadofprint SO - Mol Cell Biochem. 2026 Oct 27. doi: 10.1007/s11010-026-05791-9. PMID- 42945006 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1573-7446 (Electronic) IS - 0165-7380 (Linking) VI - 51 IP - 1 DP - 2026 Oct 27 TI - Expression, purification, and diagnostic evaluation of the recombinant matrix protein of peste des petits ruminants virus. LID - 21 [pii] LID - 10.1007/s11259-026-11593-7 [doi] AB - Peste des petits ruminants virus (PPRV) causes a highly contagious and economically devastating disease of small ruminants, particularly sheep and goats. Serological evaluation using recombinant protein-based Enzyme-linked immunosorbent assays (ELISAs) is essential for monitoring PPRV infection and assessing eradication programs. Although recombinant nucleoprotein (N) is widely used for serological evaluation, investigation of additional viral proteins may provide alternative antigenic platforms for PPRV antibody detection. The matrix (M) protein plays a central role in viral assembly by linking viral surface glycoproteins with ribonucleoprotein complexes. However, recombinant expression of full-length M protein in Escherichia coli (E. coli) is challenging because of low or truncated expression and inclusion body formation, often requiring complex refolding procedures. In this study, full-length M protein from the Nigeria 75/1 vaccine strain was successfully overexpressed as inclusion bodies (IBs) in E. coli and solubilized using N-lauroyl sarcosine (NLS) without refolding. The solubilized protein was purified by nickel-affinity chromatography, yielding 2.1 mg/L bacterial culture, and confirmed as a 38-kDa protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. An indirect ELISA was developed using purified M protein and optimized with commercial control sera. Optimal conditions were achieved with 1 microg/mL antigen and 1:20 serum dilution. Using pre-confirmed field sera, the assay demonstrated 94.59% sensitivity and 92.45% specificity at a cut-off of 0.25. Comparison with the commercial ID Screen((R)) PPR Competition ELISA showed comparable performance and strong agreement. These findings demonstrate the recombinant PPRV M protein as a feasible diagnostic antigen for PPRV antibody detection which requires further validation for large scale application. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature B.V. FAU - Gul, Zaima AU - Gul Z AD - Nuclear Institute for Agriculture and Biology College, Pakistan Institute of Engineering and Applied Sciences (NIAB-C, PIEAS), Faisalabad, 38000, Pakistan. AD - Vaccine Development Group, Animal Sciences Division, Nuclear Institute for Agriculture and Biology, P.O Box 128, Jhang Road, Faisalabad, 38000, Pakistan. FAU - Shah, Muhammad Salahuddin AU - Shah MS AD - Nuclear Institute for Agriculture and Biology College, Pakistan Institute of Engineering and Applied Sciences (NIAB-C, PIEAS), Faisalabad, 38000, Pakistan. msalahuddin786@hotmail.com. AD - Vaccine Development Group, Animal Sciences Division, Nuclear Institute for Agriculture and Biology, P.O Box 128, Jhang Road, Faisalabad, 38000, Pakistan. msalahuddin786@hotmail.com. FAU - Habib, Mudasser AU - Habib M AD - Nuclear Institute for Agriculture and Biology College, Pakistan Institute of Engineering and Applied Sciences (NIAB-C, PIEAS), Faisalabad, 38000, Pakistan. AD - Vaccine Development Group, Animal Sciences Division, Nuclear Institute for Agriculture and Biology, P.O Box 128, Jhang Road, Faisalabad, 38000, Pakistan. FAU - Zia, Muhammad Ashir AU - Zia MA AD - Department of Biological Sciences, Faculty of Science and Technology, Virtual University of Pakistan, Faisalabad, Pakistan. ashir.zia@vu.edu.pk. LA - eng GR - CRP 24026/International Atomic Energy Agency/ PT - Journal Article DEP - 20261027 PL - Switzerland TA - Vet Res Commun JT - Veterinary research communications JID - 8100520 RN - 0 (Recombinant Proteins) RN - 0 (Viral Matrix Proteins) RN - 0 (Antibodies, Viral) SB - IM MH - *Peste-des-petits-ruminants virus/genetics/immunology/isolation & purification MH - Animals MH - Recombinant Proteins/genetics/isolation & purification/immunology MH - *Peste-des-Petits-Ruminants/diagnosis/virology MH - *Viral Matrix Proteins/genetics/isolation & purification/immunology/metabolism MH - Enzyme-Linked Immunosorbent Assay/veterinary/methods MH - Escherichia coli/genetics MH - Sheep MH - Goats MH - *Goat Diseases/diagnosis/virology MH - *Sheep Diseases/diagnosis/virology MH - Antibodies, Viral/blood OTO - NOTNLM OT - ELISA OT - Inclusion bodies OT - Matrix (M) Protein OT - Peste des petits ruminants virus (PPRV) OT - Protein solubilization OT - Recombinant protein expression COIS- Declarations. Ethical approval: Blood samples were collected from sheep and goats for the purposes of this study following standard veterinary procedures. No experimental infection or invasive intervention other than blood collection was performed. All sampling procedures were carried out by trained personnel in accordance with institutional animal welfare guidelines and with efforts to minimize pain, distress, and discomfort. Ethical approval for the study was obtained from the relevant Institutional Animal Care and Use Committee (IACUC) of Animal Sciences Division, NIAB, Faisalabad, Pakistan (Approval Number: NIAB-IUCAC-2024-004). Competing interests: The authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:22 PHST- 2026/08/13 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:22 [entrez] AID - 10.1007/s11259-026-11593-7 [pii] AID - 10.1007/s11259-026-11593-7 [doi] PST - epublish SO - Vet Res Commun. 2026 Oct 27;51(1):21. doi: 10.1007/s11259-026-11593-7. PMID- 42945001 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1941-6636 (Electronic) VI - 57 IP - 1 DP - 2026 Oct 27 TI - Real-World Outcomes of First-Line Nivolumab Monotherapy in Unfavorable Carcinoma of Unknown Primary: A Single-Center Retrospective Study. LID - 245 [pii] LID - 10.1007/s12029-026-01622-8 [doi] AB - BACKGROUND: Carcinoma of unknown primary (CUP) is a heterogeneous metastatic malignancy for which the primary site remains unidentified after appropriate diagnostic evaluation. Nivolumab is approved for CUP in Japan, but detailed real-world evidence for first-line nivolumab monotherapy remains limited. METHODS: We retrospectively evaluated 10 consecutive patients with unfavorable CUP who received nivolumab monotherapy as first-line systemic therapy at a single Japanese cancer center. Patients had histologically confirmed metastatic epithelial malignancy, no identifiable primary site after clinicopathological evaluation, and no recommended site-specific treatment. Nivolumab was administered at 240 mg every 2 weeks. Responses were assessed according to RECIST version 1.1. Progression-free survival (PFS), overall survival (OS), and duration of response (DOR) were estimated using the Kaplan-Meier method. RESULTS: The median age was 71 years (range, 58-88), and 8 patients were female. Best overall response was complete response in 2 patients, partial response in 4, stable disease in 1, and progressive disease in 3, yielding an objective response rate of 60% (95% CI, 26.2-87.8) and disease control rate of 70% (95% CI, 34.8-93.3). Among the 6 responders, median DOR was 16.2 months. Median PFS and OS were 2.9 and 17.6 months, respectively. Immune-related adverse events occurred in 3 patients, including grade > = 3 events in 2. PD-L1 was not assessed in this real-world cohort. Comprehensive genomic profiling was performed in 2 patients. CONCLUSIONS: First-line nivolumab produced durable responses in a subset of selected patients with unfavorable CUP in routine Japanese practice. Given the small, retrospective, single-center cohort and absence of a comparator, these findings are descriptive and require validation in larger prospective studies. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Sagawa, Tamotsu AU - Sagawa T AUID- ORCID: 0000-0002-1994-4795 AD - Department of Gastroenterology, National Hospital Organization Hokkaido Cancer Center, 3-54 Kikusui 4-jo 2-chome, Shiroishi-ku, Sapporo, 003-0804, Hokkaido, Japan. stamotsu@jk9.so-net.ne.jp. FAU - Oiwa, Shutaro AU - Oiwa S AD - Department of Gastroenterology, National Hospital Organization Hokkaido Cancer Center, 3-54 Kikusui 4-jo 2-chome, Shiroishi-ku, Sapporo, 003-0804, Hokkaido, Japan. FAU - Minobe, Shinichiro AU - Minobe S AD - Division of Gynecologic Oncology, National Hospital Organization Hokkaido Cancer Center, Sapporo, Japan. FAU - Uesugi, Atsushi AU - Uesugi A AD - Department of Gastroenterology, National Hospital Organization Hokkaido Cancer Center, 3-54 Kikusui 4-jo 2-chome, Shiroishi-ku, Sapporo, 003-0804, Hokkaido, Japan. FAU - Yoshida, Makoto AU - Yoshida M AD - Department of Gastroenterology, National Hospital Organization Hokkaido Cancer Center, 3-54 Kikusui 4-jo 2-chome, Shiroishi-ku, Sapporo, 003-0804, Hokkaido, Japan. FAU - Nagashima, Hiroyuki AU - Nagashima H AD - Department of Gastroenterology, National Hospital Organization Hokkaido Cancer Center, 3-54 Kikusui 4-jo 2-chome, Shiroishi-ku, Sapporo, 003-0804, Hokkaido, Japan. FAU - Fujikawa, Koshi AU - Fujikawa K AD - Department of Gastroenterology, National Hospital Organization Hokkaido Cancer Center, 3-54 Kikusui 4-jo 2-chome, Shiroishi-ku, Sapporo, 003-0804, Hokkaido, Japan. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Gastrointest Cancer JT - Journal of gastrointestinal cancer JID - 101479627 RN - 31YO63LBSN (Nivolumab) RN - 0 (Antineoplastic Agents, Immunological) SB - IM MH - Humans MH - *Nivolumab/therapeutic use/pharmacology MH - Female MH - *Neoplasms, Unknown Primary/drug therapy/mortality/pathology MH - Retrospective Studies MH - Male MH - Aged MH - Aged, 80 and over MH - Middle Aged MH - Japan MH - Treatment Outcome MH - Progression-Free Survival MH - *Antineoplastic Agents, Immunological/therapeutic use OTO - NOTNLM OT - Carcinoma of unknown primary OT - Duration of response OT - First-line therapy OT - Immune checkpoint inhibitor OT - Nivolumab OT - Real-world evidence COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:22 PHST- 2026/04/01 00:00 [received] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:22 [entrez] AID - 10.1007/s12029-026-01622-8 [pii] AID - 10.1007/s12029-026-01622-8 [doi] PST - epublish SO - J Gastrointest Cancer. 2026 Oct 27;57(1):245. doi: 10.1007/s12029-026-01622-8. PMID- 42944996 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1128-045X (Electronic) IS - 1123-6337 (Print) IS - 1123-6337 (Linking) VI - 30 IP - 1 DP - 2026 Oct 27 TI - Three-week ileostomy closure after colorectal surgery: a multicenter randomized trial of technical and postoperative outcomes. LID - 10.1007/s10151-026-03416-3 [doi] LID - 163 AB - BACKGROUND: The optimal timing of temporary ileostomy closure following restorative colorectal surgery remains controversial. Although previous randomized studies have demonstrated the feasibility of early closure in carefully selected patients, the influence of closure timing on operative complexity has not been prospectively evaluated. This multicenter randomized controlled trial compared a standardized 3-week ileostomy closure strategy with conventional delayed closure, with particular emphasis on technical and postoperative outcomes. METHODS: In this prospective multicenter randomized controlled trial, 224 patients undergoing restorative colorectal resection with temporary diverting ileostomy were randomized to either early ileostomy closure at 3 weeks (n = 112) or conventional delayed closure (n = 112). Eligibility for reversal required rigorous confirmation of anastomotic healing using standardized clinical and endoscopic assessment, with selective computed tomography using rectal contrast when clinically indicated. The primary outcome was operative complexity, evaluated using operative duration, intraoperative adhesion severity, and the requirement for extended adhesiolysis. Secondary outcomes included postoperative morbidity, Clavien-Dindo complication grade, length of hospital stay, readmission, reoperation, re-diversion, and delay in adjuvant chemotherapy. RESULTS: Baseline demographic, clinical, and operative characteristics were comparable between the study groups. Early ileostomy closure was associated with significantly shorter operative duration (90 +/- 12 vs. 130 +/- 18 min; P < 0.001), substantially lower intraoperative adhesion severity (P = 0.04), and a markedly reduced requirement for extended adhesiolysis (5.4% vs. 21.4%; P < 0.001). Overall postoperative morbidity, Clavien-Dindo grade distribution, postoperative length of stay, readmission, reoperation, and mortality were comparable between groups. Delays in adjuvant chemotherapy occurred only in the early-closure group (3.6%), were limited to 1-4 weeks, and all affected patients subsequently commenced the planned oncologic treatment. CONCLUSIONS: In carefully selected patients with confirmed anastomotic healing, 3-week ileostomy closure is a technically feasible and safe strategy following restorative colorectal surgery. Compared with conventional delayed closure, reversal at 3 weeks significantly reduced operative complexity without compromising short-term postoperative outcomes. These findings support selective implementation of a standardized 3-week ileostomy closure strategy and identify operative complexity as a key determinant when selecting the optimal timing of ileostomy reversal. GOV IDENTIFIER: NCT05192018. CI - (c) 2026. The Author(s). FAU - Debakey, Y AU - Debakey Y AUID- ORCID: 0000-0002-6672-6336 AD - National Cancer Institute, Cairo University, Cairo, Egypt. y.eldebakey@cu.edu.eg. FAU - Mostafa, M S AU - Mostafa MS AUID- ORCID: 0000-0003-0485-3900 AD - Faculty of Medicine, Cairo University, Cairo, Egypt. FAU - Kilany, A AU - Kilany A AUID- ORCID: 0009-0003-5667-5234 AD - Sahel Teaching Hospital, Ministry of Health, Cairo, Egypt. FAU - Madkour, A AU - Madkour A AUID- ORCID: 0000-0001-8416-6013 AD - Faculty of Medicine, Helwan University, Cairo, Egypt. FAU - Mohamed, A R AU - Mohamed AR AUID- ORCID: 0000-0002-8164-2472 AD - Faculty of Medicine, Cairo University, Cairo, Egypt. FAU - El-Mikkawy, A AU - El-Mikkawy A AUID- ORCID: 0000-0001-8816-3290 AD - Theodor Bilharz Research Institute, Cairo, Egypt. FAU - Sheier, S S AU - Sheier SS AUID- ORCID: 0000-0001-7287-5138 AD - National Cancer Institute, Cairo University, Cairo, Egypt. FAU - Hafez, A AU - Hafez A AUID- ORCID: 0009-0000-9317-0014 AD - National Cancer Institute, Cairo University, Cairo, Egypt. FAU - ElSabbagh, O AU - ElSabbagh O AUID- ORCID: 0009-0006-2020-7035 AD - National Cancer Institute, Cairo University, Cairo, Egypt. FAU - Nofal, A AU - Nofal A AUID- ORCID: 0009-0000-5890-0261 AD - National Cancer Institute, Cairo University, Cairo, Egypt. LA - eng SI - ClinicalTrials.gov/NCT05192018 PT - Journal Article PT - Multicenter Study PT - Randomized Controlled Trial DEP - 20261027 PL - Italy TA - Tech Coloproctol JT - Techniques in coloproctology JID - 9613614 SB - IM MH - Humans MH - *Ileostomy/methods/adverse effects MH - Female MH - Middle Aged MH - Male MH - Prospective Studies MH - Time Factors MH - Treatment Outcome MH - Reoperation/statistics & numerical data MH - Postoperative Complications/etiology MH - Aged MH - Length of Stay MH - Operative Time MH - Colorectal Surgical Procedures MH - Anastomosis, Surgical/methods/adverse effects MH - Rectum/surgery MH - Tissue Adhesions/etiology PMC - PMC13714058 OTO - NOTNLM OT - Adhesion severity OT - Anastomotic assessment OT - Colorectal surgery OT - Early stoma reversal OT - Ileostomy closure OT - Operative complexity COIS- Declarations. Conflict of interest: The authors declare no conflicts of interest. Ethics approval: Approved by the Institutional Review Board of the National Cancer Institute, Cairo University (IRB approval date: 21 November 2021). EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 12:21 PHST- 2026/05/23 00:00 [received] PHST- 2026/08/10 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:21 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 10.1007/s10151-026-03416-3 [pii] AID - 3416 [pii] AID - 10.1007/s10151-026-03416-3 [doi] PST - epublish SO - Tech Coloproctol. 2026 Oct 27;30(1):163. doi: 10.1007/s10151-026-03416-3. PMID- 42944993 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1436-3771 (Electronic) IS - 1432-6981 (Linking) VI - 30 IP - 11 DP - 2026 Oct 27 TI - Periodontitis Beyond ASCVD: Emerging Cardiovascular Phenotypes in Perio-Medicine. LID - 548 [pii] LID - 10.1007/s00784-026-07234-7 [doi] AB - BACKGROUND: Periodontitis is increasingly recognized as a chronic inflammatory disease with systemic implications beyond the oral cavity. Within perio-medicine, its association with cardiovascular disease has been extensively investigated, particularly in relation to atherosclerotic cardiovascular disease (ASCVD). However, the field is now expanding toward a broader and more phenotype-specific cardiovascular framework. OBJECTIVE: This narrative review critically examines how the periodontitis-cardiovascular field has evolved from a predominantly ASCVD-centered model toward a broader spectrum of cardiovascular phenotypes, with particular emphasis on differences in evidence maturity, contemporary mechanistic concepts, and unresolved translational questions. METHODS: A structured literature search was conducted in PubMed/MEDLINE, Scopus, Web of Science, and the Cochrane Library. Evidence from mechanistic, observational, and interventional studies was qualitatively synthesized according to its contribution to established clinical associations, supportive mechanistic evidence, emerging cardiovascular phenotypes, and unresolved questions regarding causality and clinical translation. RESULTS: The association between periodontitis and coronary atherosclerotic disease represents the most mature component of the literature, with substantial observational and meta-analytic support, while ischemic cerebrovascular disease is also supported by consistent but more heterogeneous evidence. Beyond ASCVD, atrial fibrillation and heart failure currently represent the most developed emerging cardiovascular phenotypes, whereas evidence concerning abdominal aortic aneurysm and venous thromboembolism remains more preliminary and hypothesis-generating. Contemporary mechanistic models extend beyond bacteremia and systemic inflammation to include endothelial dysfunction, thrombo-inflammatory signaling, trained immunity, immune-cell trafficking, functional dysregulation of the oral microbiome, and shared biological vulnerabilities. However, these pathways differ markedly in cardiovascular-specific evidentiary maturity and should not be interpreted as equivalent evidence of human causality. Periodontal therapy can improve inflammatory biomarkers, endothelial function, selected metabolic parameters, and blood pressure in specific populations, but a persistent translational gap remains between surrogate-marker improvement and demonstrated reduction in major adverse cardiovascular events. Periodontitis may also contribute to chronic systemic inflammatory burden in susceptible individuals, although its role within cardiovascular residual inflammatory risk remains an emerging hypothesis. CONCLUSIONS: The periodontal-cardiovascular field is evolving from a predominantly ASCVD-centered model toward a phenotype-specific continuum of evidence maturity. Periodontitis is best interpreted as a potential modifier of cardiovascular vulnerability rather than as an established causal determinant. Future research should prioritize adequately powered cardiovascular-outcome trials, standardized periodontal phenotyping, and prospective validation of emerging phenotypes and mechanistic frameworks before periodontal information can be considered clinically actionable for cardiovascular prevention. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Sansavini, Martina AU - Sansavini M AD - Department of Biomedical and Neuromotor Sciences (DIBINEM), University of Bologna, Bologna, Italy. FAU - Pieretto, Giorgia AU - Pieretto G AD - Department of Neuroscience, University of Padua, Padua, Italy. FAU - Samra, Monika AU - Samra M AD - Ambedkar Center for Biomedical Research, University of Delhi, Delhi, India. FAU - Bacci, Christian AU - Bacci C AUID- ORCID: 0000-0002-6956-6227 AD - Department of Neuroscience, University of Padua, Padua, Italy. christian.bacci@unipd.it. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Germany TA - Clin Oral Investig JT - Clinical oral investigations JID - 9707115 SB - IM MH - Humans MH - Phenotype MH - *Periodontitis/complications MH - *Cardiovascular Diseases/etiology MH - *Atherosclerosis/etiology OTO - NOTNLM OT - Atherosclerosis OT - Cardiovascular disease OT - Cerebrovascular disease OT - Endothelial dysfunction OT - Inflammation OT - Periodontitis COIS- Declarations. Competing interests: The authors declare no competing interests. Ethics approval: The manuscript does not contain clinical studies or patient data. Consent to participate: Not applicable. Consent to publish: Not applicable. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:21 PHST- 2026/06/04 00:00 [received] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:21 [entrez] AID - 10.1007/s00784-026-07234-7 [pii] AID - 10.1007/s00784-026-07234-7 [doi] PST - epublish SO - Clin Oral Investig. 2026 Oct 27;30(11):548. doi: 10.1007/s00784-026-07234-7. PMID- 42944992 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1573-7292 (Electronic) IS - 1389-9600 (Print) IS - 1389-9600 (Linking) VI - 25 IP - 4 DP - 2026 Oct 27 TI - Patient experiences with hereditary cancer genetic test result release in the setting of the 21st Century Cures Act final rule. LID - 10.1007/s10689-026-00613-9 [doi] LID - 99 AB - The 21st Century Cures Act Final Rule is a federal law recently implemented in the United States of America which prompted a practice shift among genetic counselors who historically disclosed genetic testing results by directly speaking with patients. Herein, we report participant experiences following a single-center's approach of patients selecting provider-facilitated versus immediate release of hereditary cancer genetic testing results in the electronic health record preceding provider review. A voluntary survey was sent to patients who completed pre-test genetic counseling and hereditary cancer genetic testing within the University of Pennsylvania Health System. Responses were analyzed using descriptive statistics. Participants (n = 153) were predominately White (77%), female (81%), obtained Master's or Doctoral degrees (47%), and a median age of 60 (range 22-81). Participants across both result release cohorts did not differ in demographics, indication for testing, answers to knowledge-based questions or initial emotional responses. When asked about release preference for future genetic testing, most participants preferred provider-facilitated electronic portal message or a phone call. Of the participants who initially elected immediate release, only 18% (20/113) would again elect immediate release for future genetic tests. Participants across results release cohorts predominately elect for provider-facilitated release for future genetic testing, suggesting provider involvement may benefit patient disclosure experience. CI - (c) 2026. The Author(s). FAU - Bell, Cameron AU - Bell C AD - Master of Science in Genetic Counseling Program, Perelman School of Medicine, University of Pennsylvania, PA, Philadelphia, USA. FAU - Pipito, Samantha AU - Pipito S AD - Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA, 19104, USA. FAU - Mani, Ashika AU - Mani A AD - Department of Biostatistics, Epidemiology and Informatics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. FAU - Anantharajah, Arravinth AU - Anantharajah A AD - Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA, 19104, USA. FAU - Apostolico, Taylor AU - Apostolico T AD - Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA, 19104, USA. FAU - Arnold, Angela G AU - Arnold AG AD - Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA, 19104, USA. FAU - Cappadocia, Jacqueline AU - Cappadocia J AD - Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA, 19104, USA. FAU - Clark, Dana Farengo AU - Clark DF AD - Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA, 19104, USA. FAU - Heller, Melissa AU - Heller M AD - Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA, 19104, USA. FAU - Long, Jessica M AU - Long JM AD - Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA, 19104, USA. FAU - Mann, Derek AU - Mann D AD - Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA, 19104, USA. FAU - McKennon, Emma AU - McKennon E AD - Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA, 19104, USA. FAU - Mercado, Joanna AU - Mercado J AD - Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA, 19104, USA. FAU - Powers, Jacquelyn AU - Powers J AD - Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA, 19104, USA. FAU - Raper, Anna AU - Raper A AD - Division of Translational Medicine & Human Genetics, Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA. FAU - Spielman, Kelsey AU - Spielman K AD - Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA, 19104, USA. FAU - Katona, Bryson W AU - Katona BW AD - Division of Gastroenterology and Hepatology, Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA. FAU - Maxwell, Kara AU - Maxwell K AD - Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA, 19104, USA. FAU - Nathanson, Katherine L AU - Nathanson KL AD - Division of Translational Medicine & Human Genetics, Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA. FAU - Rohanizadegan, Mersedeh AU - Rohanizadegan M AD - Division of Translational Medicine & Human Genetics, Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA. FAU - Shah, Payal D AU - Shah PD AD - Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA, 19104, USA. FAU - Bradbury, Angela AU - Bradbury A AD - Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA, 19104, USA. FAU - Domchek, Susan M AU - Domchek SM AD - Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA, 19104, USA. FAU - Mueller, Rebecca AU - Mueller R AD - Master of Science in Genetic Counseling Program, Perelman School of Medicine, University of Pennsylvania, PA, Philadelphia, USA. AD - Division of Translational Medicine & Human Genetics, Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA. FAU - Ebrahimzadeh, Jessica E AU - Ebrahimzadeh JE AUID- ORCID: 0000-0002-1505-9625 AD - Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Health System, Philadelphia, PA, 19104, USA. jebra@pennmedicine.upenn.edu. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Fam Cancer JT - Familial cancer JID - 100898211 SB - IM MH - Humans MH - Female MH - *Genetic Testing/legislation & jurisprudence MH - Middle Aged MH - Adult MH - Aged MH - *Genetic Counseling/legislation & jurisprudence/psychology MH - Return of Individual Research Results MH - Male MH - United States MH - Aged, 80 and over MH - Young Adult MH - Surveys and Questionnaires MH - Electronic Health Records PMC - PMC13714062 OTO - NOTNLM OT - 21st Century Cures Act OT - Electronic health record (EHR) OT - Genetic counseling OT - Hereditary cancer genetic testing OT - Patient-centered care OT - Results communication COIS- Declarations. Conflict of interest: The authors declare no conflict of interest. Ethical approval: This study was approved by the University of Pennsylvania Institutional Review Board (IRB) and the University of Pennsylvania Abrahamson Cancer Center Data and Safety Monitoring Committee (DSMC). All institutions affiliated with Penn Medicine received local IRB approval. As required by these bodies, informed consent to participate in this study was obtained from all participants. The IRB granted a waiver of written documentation of informed consent due to their determination of minimal risk to participants. Individual-level participant data was de-identified before analysis. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 12:21 PHST- 2026/06/19 00:00 [received] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:21 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 10.1007/s10689-026-00613-9 [pii] AID - 613 [pii] AID - 10.1007/s10689-026-00613-9 [doi] PST - epublish SO - Fam Cancer. 2026 Oct 27;25(4):99. doi: 10.1007/s10689-026-00613-9. PMID- 42944991 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1573-2959 (Electronic) IS - 0167-6369 (Linking) VI - 198 IP - 11 DP - 2026 Oct 27 TI - Domestic and wild animals as sentinels for environmental monitoring of emerging contaminants: a One Health framework for biomonitoring and human exposure assessment. LID - 1258 [pii] LID - 10.1007/s10661-026-16026-1 [doi] AB - Emerging contaminants are increasingly detected across environmental, domestic, agricultural and food-chain matrices, where low-dose chronic exposure, mixtures, and delayed toxicological outcomes challenge conventional human-centred surveillance. This critical narrative review and monitoring-framework article uses structured keyword selection and literature mapping informed by SANRA quality criteria to integrate evidence on companion animals, livestock and wildlife as sentinels of per- and polyfluoroalkyl substances (PFAS), endocrine disruptors, micro- and nanoplastics, pesticides, pharmaceuticals, and food-chain contaminants. Companion animals can support reconstruction of shared indoor and lifestyle exposures; livestock can identify contamination at the interface between feed, water, soil, and animal-derived foods; and wildlife can integrate ecosystem, trophic, and landscape-level exposure. The proposed framework outlines monitoring modules, normalization considerations, biospecimen-banking options, and tiered operational triggers connecting sentinel detection to environmental verification and targeted human biomonitoring. Two application models-a hypothetical localized PFAS plume involving waterfowl and dairy cattle and an evidence-informed Arctic sled dog model for chronic long-range transported mixtures-illustrate how animal signals can support exposure reconstruction, hotspot identification, and public-health prioritization. Animal sentinel evidence should be interpreted as early warning and triangulating evidence, not as stand-alone proof of human health risk. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature Switzerland AG. FAU - Britti, Domenico AU - Britti D AUID- ORCID: 0000-0001-7355-8791 AD - Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro, 88100, Catanzaro, Italy. britti@unicz.it. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Netherlands TA - Environ Monit Assess JT - Environmental monitoring and assessment JID - 8508350 RN - 0 (Environmental Pollutants) SB - IM MH - Animals MH - *Environmental Monitoring/methods MH - Humans MH - Animals, Wild MH - *Environmental Exposure/statistics & numerical data/analysis MH - *Environmental Pollutants/analysis MH - *Biological Monitoring MH - Animals, Domestic MH - One Health MH - Sentinel Species OTO - NOTNLM OT - Companion animals OT - Exposure reconstruction OT - Food-chain transfer OT - Livestock OT - Sentinel surveillance OT - Wildlife biomonitoring COIS- Declarations. Ethics approval: This is not applicable. This narrative review did not involve original studies with human participants or animals performed by the author. Consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The author declares no competing interests. Clinical trial number: Not applicable. Use of AI-assisted tools: AI-assisted tools, if used, were limited to language polishing, formatting support, and refinement of schematic presentation. No AI tool was used to generate autonomous scientific content, data, interpretations, or conclusions. The author reviewed and approved the final manuscript and accepts full responsibility for its content. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:21 PHST- 2026/08/09 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:21 [entrez] AID - 10.1007/s10661-026-16026-1 [pii] AID - 10.1007/s10661-026-16026-1 [doi] PST - epublish SO - Environ Monit Assess. 2026 Oct 27;198(11):1258. doi: 10.1007/s10661-026-16026-1. PMID- 42944986 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261027 IS - 1436-5073 (Electronic) IS - 0026-3672 (Linking) VI - 193 IP - 11 DP - 2026 Oct 27 TI - Electrokinetic detection of organic contaminants using molecularly imprinted polymer particles in microchannels. LID - 759 [pii] LID - 10.1007/s00604-026-08492-8 [doi] AB - A novel analytical technique for organic contaminants was developed based on the electrokinetic motion of submicron molecularly imprinted polymer (MIP) particles. As the electrokinetic velocity of the MIP particles changes after selective adsorption of organic contaminant from the aqueous sample, the concentration of the contaminant can be determined by measuring the variation of the electrokinetic velocity of the MIP particles in a microchannel. Following the investigations of the synthesis of size-controlled MIP particles, characterization of the adsorption capability, and mechanism of the electrokinetics of the porous particles, the electrokinetic analytical technique was demonstrated by applying this method in analyzing sulfamethoxazole (SMX) as a representative antibiotic organic contaminant. This technique showed an excellent linear response to the logarithmic SMX concentrations in the range of 1 to 1000 microM with LOD = 0.17 microM, which also indicated promising adaptability for practical applications with the recovery of 76-101% for lake water and 93-111% for sea water. This method can be readily extended to other organic contaminants by employing the corresponding target molecules as templates during MIP synthesis. Owing to its simplicity and efficiency, the electrokinetic analytical approach holds great potential for miniaturization into portable onsite sensors by integrating particle velocity measurement techniques. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature. FAU - Li, Mengqi AU - Li M AD - Department of Marine Engineering, Dalian Maritime University, Dalian, 116026, China. FAU - Ma, Wenjun AU - Ma W AD - Department of Environmental Science and Engineering, Dalian Maritime University, No.1 Linghai Road, Dalian, 116026, China. FAU - Yang, Gengdian AU - Yang G AD - Department of Marine Engineering, Dalian Maritime University, Dalian, 116026, China. FAU - Sun, Ya AU - Sun Y AD - Department of Environmental Science and Engineering, Dalian Maritime University, No.1 Linghai Road, Dalian, 116026, China. FAU - Shi, Yawei AU - Shi Y AD - Department of Environmental Science and Engineering, Dalian Maritime University, No.1 Linghai Road, Dalian, 116026, China. ywshi@dlmu.edu.cn. LA - eng GR - 52401413/National Natural Science Foundation of China/ GR - 2024YFB4206601/National Key Research and Development Program of China/ GR - 2025-MSLH-085/Liaoning Provincial Science and Technology Joint Program project/ PT - Journal Article DEP - 20261027 PL - Austria TA - Mikrochim Acta JT - Mikrochimica acta JID - 7808782 SB - IM OTO - NOTNLM OT - Electrokinetics OT - Microfluidic chip OT - Molecularly imprinted polymer OT - Organic contaminant COIS- Declarations. Competing interests: The authors declare no competing interests. AI disclosure: The authors declare that no artificial intelligence (AI) tools were used in the preparation of the current version of this manuscript, including the figures and graphical abstract. Supporting information: The Supporting Information is available. Experimental details for the adsorption tests; experimental details for Measurement of electrokinetic velocity; experimental details for the Evaluation of detection performance; schematic diagram of the preparation of the MIP particle; characterization of the MIP, NIP, and e-MIP particles; adsorption kinetics; adsorption isotherms; water quality index test results; HPLC analysis. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:21 PHST- 2026/07/21 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:21 [entrez] AID - 10.1007/s00604-026-08492-8 [pii] AID - 10.1007/s00604-026-08492-8 [doi] PST - epublish SO - Mikrochim Acta. 2026 Oct 27;193(11):759. doi: 10.1007/s00604-026-08492-8. PMID- 42944984 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 0948-5023 (Electronic) IS - 0948-5023 (Linking) VI - 32 IP - 11 DP - 2026 Oct 27 TI - Interaction dynamics of boswellic acid analogs with TNFR1 and TNFR2: a molecular docking and simulation study. LID - 450 [pii] LID - 10.1007/s00894-026-07019-5 [doi] AB - CONTEXT: Tumor necrosis factor receptors (TNFR1 and TNFR2) play important roles in inflammatory and immune signaling and are associated with several inflammatory and autoimmune diseases. Despite the reported anti-inflammatory activity of boswellic acid, less is known about the interactions of structurally related analogs with TNFR1 and TNFR2. In this study, boswellic acid and structurally related analogs were evaluated computationally against TNFR1 and TNFR2, with the interaction profiles assessed separately for each receptor subtype. Based on the overall computational results, paeonenoide B (B3) and paeonenoide C (B4) were selected for further investigation. Molecular dynamics simulations and MM-GBSA calculations indicated that both compounds remained associated with the receptors throughout the simulations, although differences in interaction patterns and conformational behavior were observed between the TNFR1 and TNFR2 systems. These findings may be useful for future experimental evaluation of boswellic acid-related compounds. METHODS: Boswellic acid-like compounds were identified by structural similarity searching in PubChem and evaluated using drug-likeness and ADME/T analyses performed with ADMETlab 2.0. Molecular docking was carried out against TNFR1 (PDB ID: 1EXT) and TNFR2 (PDB ID: 3ALQ) using UCSF Chimera and AutoDock Vina. Selected complexes were subjected to molecular dynamics simulations following ligand parameterization with Gaussian 16 and AmberTools16. Three independent 500-ns simulations were performed for each system in explicit solvent. Structural analyses were performed using CPPTRAJ and PyMOL, and binding free energies were estimated using the MM-GBSA method. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Ozmaldar, Aydin AU - Ozmaldar A AUID- ORCID: 0000-0002-0664-0100 AD - Department of IT, Krosgen Biotechnology, Istanbul, 34750, Turkey. FAU - Akkaya, Hatice AU - Akkaya H AUID- ORCID: 0000-0001-7276-6919 AD - Department of Biochemistry, Faculty of Pharmacy, University of Health Sciences, Istanbul, 34668, Turkey. hatice.akkaya@sbu.edu.tr. LA - eng PT - Journal Article DEP - 20261027 PL - Germany TA - J Mol Model JT - Journal of molecular modeling JID - 9806569 RN - 0 (Triterpenes) RN - 0 (Receptors, Tumor Necrosis Factor, Type I) RN - 631-69-6 (boswellic acid) RN - 0 (Receptors, Tumor Necrosis Factor, Type II) SB - IM MH - *Triterpenes/chemistry/pharmacology/metabolism MH - *Molecular Dynamics Simulation MH - *Molecular Docking Simulation MH - *Receptors, Tumor Necrosis Factor, Type I/chemistry/metabolism MH - *Receptors, Tumor Necrosis Factor, Type II/chemistry/metabolism MH - Protein Binding MH - Humans MH - Binding Sites OTO - NOTNLM OT - Boswellic acid analogs OT - MM-GBSA OT - Molecular dynamics simulation OT - TNFR1 OT - TNFR2 COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:20 PHST- 2026/06/08 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:20 [entrez] AID - 10.1007/s00894-026-07019-5 [pii] AID - 10.1007/s00894-026-07019-5 [doi] PST - epublish SO - J Mol Model. 2026 Oct 27;32(11):450. doi: 10.1007/s00894-026-07019-5. PMID- 42944981 OWN - NLM STAT- Publisher LR - 20261027 IS - 2364-1746 (Electronic) IS - 2364-1754 (Linking) DP - 2026 Oct 27 TI - The Impact of Antibiotic Initiation Timing in Children with Severe Community-Acquired Pneumonia: A Post-Hoc Analysis of an Observational Study. LID - 10.1007/s41030-026-00398-3 [doi] AB - INTRODUCTION: The aim of this study was to examine the association between early antibiotic initiation and clinical outcomes in children admitted to the paediatric intensive care unit (PICU) with severe community-acquired pneumonia (CAP). METHODS: We conducted a post-hoc analysis of a multicentre observational study across nine Asian PICUs. Children with severe CAP were categorized into early ( 6 h) antibiotic initiation groups (early and late antibiotic groups, respectively) relative to PICU admission. The primary outcome was PICU mortality; secondary outcomes were 28-day ventilation-free days (VFD) and intensive care unit-free days (IFD). Multivariable analyses adjusted for country as fixed effect, age, Paediatric Index of Mortality 3 (PIM 3) score and comorbidities, when feasible. RESULTS: Among the 544 paediatric patients included in the analysis, median age was 14.0 [interquartile range (IQR) 4.0-34.0] months and the PIM 3 score was 0.3 (IQR 0.0-6.8) %. Viral and bacterial pathogens were isolated in 206 (37.9%) and 145 (26.7%) patients, respectively. Almost all patients [537 (98.7%)] received antibiotics, with 475 (87.3%) and 62 (11.4%) patients in the early and late antibiotic groups, respectively. Late antibiotic initiation was associated with higher PICU mortality [adjusted odds ratio (aOR) 5.19, 95% confidence interval (CI) 1.94-13.88, P < 0.01], septic shock requiring inotropic support (aOR 3.25, 95% CI 1.45-7.28, P < 0.01) and multi-organ failure (aOR 4.60, 95% CI 2.11-10.04, P < 0.01). No significant associations were observed between late antibiotic initiation and VFD or IFD. CONCLUSION: In critically ill children with severe CAP, initiating antibiotics within 6 h of PICU admission was associated with improved outcomes. This 6-h window may serve as a practical benchmark for quality improvement initiatives. CI - (c) 2026. The Author(s). FAU - Loh, Sin Wee AU - Loh SW AUID- ORCID: 0000-0002-0321-8991 AD - Children's Intensive Care Unit, Department of Paediatric Subspecialties, KK Women's and Children's Hospital, 100 Bukit Timah Road, Singapore, 229899, Singapore. xinweiloh87@gmail.com. AD - Duke-NUS Medical School, Singapore, Singapore. xinweiloh87@gmail.com. FAU - Wong, Judith Ju-Ming AU - Wong JJ AD - Children's Intensive Care Unit, Department of Paediatric Subspecialties, KK Women's and Children's Hospital, 100 Bukit Timah Road, Singapore, 229899, Singapore. AD - Duke-NUS Medical School, Singapore, Singapore. FAU - Abbas, Qalab AU - Abbas Q AD - Aga Khan University Hospital, Karachi, Pakistan. FAU - Angurana, Suresh Kumar AU - Angurana SK AD - Post Graduate Institute of Medical Education and Research, Chandigarh, Chandigarh, India. FAU - Cho, Hwa Jin AU - Cho HJ AD - Chonnam National University Children's Hospital and Medical School, Gwangju, South Korea. FAU - Dang, Hongxing AU - Dang H AD - Children's Hospital of Chongqing Medical University, Chongqing, China. FAU - Efar, Pustika AU - Efar P AD - Harapan Kita Women's and Children's Hospital, West Jakarta, Indonesia. FAU - Phan, Phuc Huu AU - Phan PH AD - Vietnam National Children's Hospital, Hanoi, Vietnam. FAU - Lee, Pei Chuen AU - Lee PC AD - University Kebangsaan Malaysia Medical Center, Kuala Lumpur, Malaysia. FAU - Liu, Chunfeng AU - Liu C AD - Shengjing Hospital of China Medical University, Shenyang, China. FAU - Fan, Lijia AU - Fan L AD - National University Hospital, Singapore, Singapore. FAU - Pukdeetraipop, Minchaya AU - Pukdeetraipop M AD - King Chulalongkorn Memorial Hospital, Bangkok, Thailand. FAU - Wang, Justin Qi Yuee AU - Wang JQY AD - University Malaya Medical Centre, Kuala Lumpur, Malaysia. FAU - Yuliarto, Saptadi AU - Yuliarto S AD - Saiful Anwar Hospital, Malang, Indonesia. FAU - Zhu, Mary Xuemei AU - Zhu MX AD - Children's Hospital of Fudan University, Shanghai, China. FAU - Sultana, Rehena AU - Sultana R AD - Centre for Quantitative Medicine, Duke-NUS Medical School, Singapore, Singapore. FAU - Lee, Jan Hau AU - Lee JH AD - Children's Intensive Care Unit, Department of Paediatric Subspecialties, KK Women's and Children's Hospital, 100 Bukit Timah Road, Singapore, 229899, Singapore. AD - Duke-NUS Medical School, Singapore, Singapore. CN - Paediatric Acute Critical Care Asian Network (PACCMAN) LA - eng GR - AM/TP003/2018/SingHealth/ PT - Journal Article DEP - 20261027 PL - United States TA - Pulm Ther JT - Pulmonary therapy JID - 101687144 OTO - NOTNLM OT - Antibiotics OT - Community-acquired pneumonia OT - Paediatric intensive care unit OT - Paediatrics OT - Pneumonia COIS- Declarations. Conflict of Interest: Jan Hau Lee is an editorial board member of Pulmonary and Therapy. Jan Hau Lee was not involved in the selection of peer reviewers for the manuscript nor any of the subsequent editorial decisions. Sin Wee Loh, Judith Ju-Ming Wong, Qalab Abbas, Suresh Kumar Angurana, Hwa Jin Cho, Hongxing Dang, Pustika Efar, Phuc Huu Phan, Pei Chuen Lee, Chunfeng Liu, Lijia Fan, Minchaya Pukdeetraipop, Justin Qi Yuee Wang, Saptadi Yuliarto, Mary Xuemei Zhu and Rehena Sultana have nothing to disclose. Ethical Approval: The study was conducted in accordance with the Declaration of Helsinki. Ethical approval for the original study was obtained from the Singhealth Centralised Institutional Review Board (CIRB) in Singapore (reference number 2019/2825) and the institutional review boards of all participating centres. Informed consent was obtained from parents or legal representatives where required. Data were securely stored in accordance with local regulations, merged and analysed at the coordinating site (KK Women's and Children's Hospital, Singapore). This study was funded by the Singhealth Foundation grant (reference number: AM/TP003/2018, PI: JHL). Clinical management of patients was at the discretion of the treating physicians at each site. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:20 PHST- 2026/08/04 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:20 [entrez] AID - 10.1007/s41030-026-00398-3 [pii] AID - 10.1007/s41030-026-00398-3 [doi] PST - aheadofprint SO - Pulm Ther. 2026 Oct 27. doi: 10.1007/s41030-026-00398-3. PMID- 42944980 OWN - NLM STAT- Publisher LR - 20261027 IS - 2364-1746 (Electronic) IS - 2364-1754 (Linking) DP - 2026 Oct 27 TI - Metabolic Syndrome in Adults with Polysomnography-Confirmed Obstructive Sleep Apnoea: A Systematic Review and Meta-analysis. LID - 10.1007/s41030-026-00391-w [doi] AB - BACKGROUND/OBJECTIVE: Obstructive sleep apnoea (OSA) and metabolic syndrome (MetS) are prevalent cardio-metabolic conditions which frequently coexist. Growing evidence indicates a positive association between OSA and MetS, but up-to-date global data are lacking, leaving the true strength of the association uncertain. Therefore, this systematic review and meta-analysis aimed to provide an updated and comprehensive synthesis of evidence on the risk of MetS in adults with polysomnography (PSG)-confirmed OSA. METHODS: A systematic review and three-level random-effects meta-analysis were conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines. Searches were performed of the Ovid MEDLINE (Medical Literature Analysis and Retrieval System Online), CINAHL (Cumulative Index to Nursing and Allied Health Literature), Cochrane and Embase databases, yielding 8072 records. Studies reporting either odds ratios (OR) for MetS in adults with PSG-confirmed OSA compared to those without OSA, or with data to calculate these, were eligible for inclusion. Risk of bias was assessed using the RoBANS (Risk of Bias Assessment tool for Non-randomised Studies) tool. Subgroup analyses and meta-regression were performed to explore sources of heterogeneity. RESULTS: A total of 68 studies from 24 countries with 74 relevant effect sizes were included (24,663 adults with PSG-confirmed OSA; 11,423 without OSA). Adults with OSA had over threefold higher odds of MetS compared with those without (odds ratio 3.33, 95% confidence interval 2.86-3.87; p < 0.0001). Substantial heterogeneity was noted (I(2) = 73.8%). Study design, geographic region, MetS definition/criteria, Apnoea-Hypopnea Index OSA diagnostic threshold and publication year significantly moderated the pooled estimate (all p < 0.05). A larger body mass index difference between groups was associated with higher odds of MetS (beta = 0.14, t = 4.03, p < 0.001), whereas a higher proportion of male participants was associated with lower odds of MetS (beta = - 0.01, t = - 2.81, p < 0.010). CONCLUSIONS: Adults with PSG-confirmed OSA have over threefold higher odds of having MetS, consistently across diverse populations. Thus, early and routine metabolic screening is recommended for all adults with OSA. Future research should incorporate longitudinal and genetic data to explore causal links between OSA and MetS. PROSPERO REGISTRATION NUMBER: CRD420251073055. CI - (c) 2026. The Author(s). FAU - Pant, Suman AU - Pant S AUID- ORCID: 0000-0001-6135-7095 AD - Discoveries in Life Sciences Research Centre, Coventry University, Richard Crossman Building, 4th Floor, Coventry, West Midlands, CV1 5FB, UK. pants2@uni.coventry.ac.uk. FAU - Kyrou, Ioannis AU - Kyrou I AD - Discoveries in Life Sciences Research Centre, Coventry University, Richard Crossman Building, 4th Floor, Coventry, West Midlands, CV1 5FB, UK. AD - Warwickshire Institute for the Study of Diabetes, Endocrinology and Metabolism (WISDEM), University Hospitals Coventry and Warwickshire NHS Trust, Coventry, West Midlands, CV2 2DX, UK. AD - Institute for Cardiometabolic Medicine, University Hospitals Coventry and Warwickshire NHS Trust, Coventry, West Midlands, CV2 2DX, UK. AD - Aston Medical School, College of Health and Life Sciences, Aston University, Birmingham, West Midlands, B4 7ET, UK. AD - College of Health, Psychology and Social Care, University of Derby, Derby, Derbyshire, DE22 1GB, UK. FAU - Dallaway, Alexander AU - Dallaway A AD - Warwickshire Institute for the Study of Diabetes, Endocrinology and Metabolism (WISDEM), University Hospitals Coventry and Warwickshire NHS Trust, Coventry, West Midlands, CV2 2DX, UK. AD - School of Allied and Public Health Sciences, Faculty of Medicine and Health Sciences, University of Wolverhampton, Wolverhampton, West Midlands, WV1 1LY, UK. FAU - Kite, Chris AU - Kite C AD - Warwickshire Institute for the Study of Diabetes, Endocrinology and Metabolism (WISDEM), University Hospitals Coventry and Warwickshire NHS Trust, Coventry, West Midlands, CV2 2DX, UK. AD - School of Allied and Public Health Sciences, Faculty of Medicine and Health Sciences, University of Wolverhampton, Wolverhampton, West Midlands, WV1 1LY, UK. FAU - Lagojda, Lukasz AU - Lagojda L AD - Warwickshire Institute for the Study of Diabetes, Endocrinology and Metabolism (WISDEM), University Hospitals Coventry and Warwickshire NHS Trust, Coventry, West Midlands, CV2 2DX, UK. AD - Sheffield Centre for Health and Related Research (SCHARR), School of Medicine and Population Health, Sheffield, South Yorkshire, S1 4DA, UK. FAU - Asad, Ali AU - Asad A AD - Department of Respiratory Medicine, University Hospitals Coventry and Warwickshire NHS Trust, Coventry, West Midlands, CV2 2DX, UK. FAU - Dhungana, Ashesh AU - Dhungana A AD - Department of Pulmonary, Critical Care and Sleep Medicine, National Academy of Medical Sciences (NAMS)-Bir Hospital, Mahaboudha, Kathmandu, 44600, Bagmati Province, Nepal. FAU - Pangeni, Raju AU - Pangeni R AD - Department of Pulmonary, Critical Care and Sleep Medicine, Hospital for Advanced Medicine and Surgery (HAMS), Mandikhatar, Kathmandu, 44600, Bagmati Province, Nepal. FAU - Randeva, Harpal Singh AU - Randeva HS AD - Discoveries in Life Sciences Research Centre, Coventry University, Richard Crossman Building, 4th Floor, Coventry, West Midlands, CV1 5FB, UK. AD - Warwickshire Institute for the Study of Diabetes, Endocrinology and Metabolism (WISDEM), University Hospitals Coventry and Warwickshire NHS Trust, Coventry, West Midlands, CV2 2DX, UK. AD - Institute for Cardiometabolic Medicine, University Hospitals Coventry and Warwickshire NHS Trust, Coventry, West Midlands, CV2 2DX, UK. AD - Warwick Medical School, University of Warwick, Coventry, West Midlands, CV4 7AL, UK. FAU - Kurmi, Om Prakash AU - Kurmi OP AD - Department of Pulmonary, Critical Care and Sleep Medicine, National Academy of Medical Sciences (NAMS)-Bir Hospital, Mahaboudha, Kathmandu, 44600, Bagmati Province, Nepal. AD - Division of Respirology, Department of Medicine, McMaster University, Hamilton, ON, L8N 3Z5, Canada. AD - Lincoln Institute for Rural and Coastal Health, University of Lincoln, Brayford Pool, Lincoln, Lincolnshire, LN6 7TS, UK. LA - eng PT - Journal Article PT - Systematic Review DEP - 20261027 PL - United States TA - Pulm Ther JT - Pulmonary therapy JID - 101687144 OTO - NOTNLM OT - Adults OT - Cardio-metabolic risk OT - Meta-analysis OT - Metabolic syndrome OT - OSA OT - Obstructive sleep apnoea OT - Systematic review COIS- Declarations. Conflict of Interest: Om Prakash Kurmi is an Editorial Board member of Pulmonary Therapy. He was not involved in the selection of peer reviewers for the manuscript nor any of the subsequent editorial decisions. Suman Pant, Ioannis Kyrou, Alexander Dallaway, Chris Kite, Lukasz Lagojda, Ali Asad, Ashesh Dhungana, Raju Pangeni and Harpal Singh Randeva declare no personal, financial, commercial or academic conflicts of interest. Ethical Approval: This article is based on previously conducted studies and does not contain any new studies with human participants or animals performed by any of the authors. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:20 PHST- 2026/09/06 00:00 [received] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:20 [entrez] AID - 10.1007/s41030-026-00391-w [pii] AID - 10.1007/s41030-026-00391-w [doi] PST - aheadofprint SO - Pulm Ther. 2026 Oct 27. doi: 10.1007/s41030-026-00391-w. PMID- 42944973 OWN - NLM STAT- Publisher LR - 20261027 IS - 1179-1926 (Electronic) IS - 0312-5963 (Linking) DP - 2026 Oct 27 TI - Paediatric Fentanyl Population Pharmacokinetics: A Scoping Review of Published Models. LID - 10.1007/s40262-026-01706-5 [doi] AB - OBJECTIVE AND PURPOSE: Fentanyl is a highly lipophilic opioid widely used within paediatric peri-operative and intensive-care settings, yet dosing is commonly titrated empirically because of substantial pharmacokinetic variability across developmental stages and clinical contexts. Population pharmacokinetic (PopPK) models provide a quantitative framework to characterise variability, but these have not been systematically synthesised. This scoping review aimed to identify and summarise published paediatric PopPK models of fentanyl, to compare model structures and covariate effects. METHODS: We searched Embase and MEDLINE databases from database inception to 17 February 2026 for paediatric PopPK models of fentanyl. Two reviewers independently screened papers and extracted data in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) guidelines. RESULTS: Fourteen paediatric PopPK models of fentanyl were identified. Infants (64%, 9/14) and patient populations spanning multiple age groups (e.g., inclusion of infants and children) (64%, 9/14) were most frequently studied. Intravenous fentanyl was modelled in all studies. Two-compartment structures predominated (79%, 11/14). Body weight was the most consistently retained covariate (79%, 11/14), while maturation functions based on postnatal or postmenstrual age were frequently required in neonatal and infant cohorts (50%, 5/10). Other covariates (e.g., obesity, concomitant CYP3A modulators, and genetic factors) were variably explored and inconsistently retained. CONCLUSION: Within the existing fentanyl literature, important evidence gaps remain, particularly beyond intravenous administration. Existing models highlight body weight and developmental maturation as principal determinants of clearance and distribution. Evidence remains limited to intravenous administration, with no model data of non-intravenous routes and under-representation of dynamic covariates and external validation. Addressing these gaps will support robust model-informed precision dosing of fentanyl in children. CI - (c) 2026. The Author(s). FAU - Hughes, Josephine J AU - Hughes JJ AUID- ORCID: 0009-0008-4715-7597 AD - School of Pharmacy, Faculty of Medicine and Health, The University of Sydney, A15 Pharmacy and Bank Building, Science Road, Camperdown, NSW, 2006, Australia. FAU - Nam, Gi Kon AU - Nam GK AUID- ORCID: 0009-0001-5017-2869 AD - School of Pharmacy, Faculty of Medicine and Health, The University of Sydney, A15 Pharmacy and Bank Building, Science Road, Camperdown, NSW, 2006, Australia. FAU - Stokes, Michael A AU - Stokes MA AUID- ORCID: 0009-0005-6615-4998 AD - School of Pharmacy, Faculty of Medicine and Health, The University of Sydney, A15 Pharmacy and Bank Building, Science Road, Camperdown, NSW, 2006, Australia. AD - Kids Critical Care Research, Children's Hospital at Westmead, Westmead, NSW, Australia. FAU - Sandaradura, Indy AU - Sandaradura I AUID- ORCID: 0000-0002-9457-8262 AD - Centre for Infectious Diseases and Microbiology, Westmead Hospital, Sydney, NSW, Australia. AD - Faculty of Medicine and Health, Westmead Clinical School, The University of Sydney, Sydney, NSW, Australia. AD - Institute of Clinical Pathology and Medical Research, New South Wales Health Pathology, Westmead Hospital, Sydney, NSW, Australia. FAU - Festa, Marino S AU - Festa MS AUID- ORCID: 0000-0001-6541-7841 AD - Kids Critical Care Research, Children's Hospital at Westmead, Westmead, NSW, Australia. FAU - Stocker, Sophie L AU - Stocker SL AUID- ORCID: 0000-0002-2114-587X AD - School of Pharmacy, Faculty of Medicine and Health, The University of Sydney, A15 Pharmacy and Bank Building, Science Road, Camperdown, NSW, 2006, Australia. sophie.stocker@sydney.edu.au. AD - Department of Clinical Pharmacology and Toxicology, St. Vincent's Hospital Sydney, Sydney, NSW, 2010, Australia. sophie.stocker@sydney.edu.au. AD - St. Vincent's Clinical Campus, Faculty of Medicine, University of New South Wales, Sydney, NSW, 2052, Australia. sophie.stocker@sydney.edu.au. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Switzerland TA - Clin Pharmacokinet JT - Clinical pharmacokinetics JID - 7606849 SB - IM COIS- Declarations. Conflicts of interest/competing interests: Sophie Stocker is an Editorial Board member of Clinical Pharmacokinetics and was not involved in the selection of peer reviewers for the manuscript nor any of the subsequent editorial decisions. No other authors declare any other conflicts of interest. Ethics approval: Not applicable. Consent to participate: Not applicable. Consent for publication: Not applicable. Availability of data and material code: Not applicable. Authors' contributions: JJH, MAS, SLS, MSF, and IS conceived the article and determined the study design. JJH, GKN, MAS, and SLS participated in article screening and collected the data. JJH drafted the manuscript and analysed the results. All authors contributed to critical review of the manuscript draft and approved the final version of the manuscript. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:20 PHST- 2026/05/01 00:00 [received] PHST- 2026/09/01 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:20 [entrez] AID - 10.1007/s40262-026-01706-5 [pii] AID - 10.1007/s40262-026-01706-5 [doi] PST - aheadofprint SO - Clin Pharmacokinet. 2026 Oct 27. doi: 10.1007/s40262-026-01706-5. PMID- 42944971 OWN - NLM STAT- Publisher LR - 20261027 IS - 1573-9686 (Electronic) IS - 0090-6964 (Linking) DP - 2026 Oct 27 TI - Hemodynamic Analysis of a Repaired Ascending Aorta with Preserved Aortic Root. LID - 10.1007/s10439-026-04375-4 [doi] AB - PURPOSE: To evaluate the hemodynamic impact of restoring a normal sino-tubular junction (STJ) following a novel Hegar dilator-based procedure in patients undergoing root-sparing ascending thoracic aortic aneurysm (ATAA) repair using computational modeling. METHODS: We retrospectively selected an ATAA patient who underwent pre- and postoperative gated computed tomography angiography (CTA). We developed a novel workflow to segment the lumen, thick-walled aorta, and aortic valve from CTA images for subsequent blood flow analysis using computational fluid dynamics (CFD) and fluid-structure interaction (FSI). Morphological and hemodynamic characteristics of the root were quantified and compared against those of a control subject. Sensitivity analyses were performed to evaluate the impact of graft properties, leaflet material parameters and heterogeneity, wall compliance, external tissue support, and solid domain annular boundary conditions. RESULTS: Both CFD and FSI results showed that the postoperative geometry reconstructed with a normal STJ profile reintroduces sinus vortices during peak systole, similar to the control subject, which were absent pre-surgery. Accounting for aortic valve leaflets in FSI studies yielded a similar spatial patterning of the high-velocity jet and wall shear stress compared to the CFD data. However, FSI resulted in substantially elevated velocities, time-averaged wall shear stress (TAWSS), and energy dissipation, likely due to the dynamically changing orifice area and differing profiles of the left ventricular outflow tract (LVOT). CONCLUSION: We demonstrated that restoring a normal sinus shape and STJ profile during ascending aortic repair may have potential hemodynamic benefits. The study also highlights the model's sensitivities, particularly the LVOT shape and leaflet morphology and mobility, and may assist planning STJ reconstruction to yield optimal hemodynamics before intervention. CI - (c) 2026. The Author(s) under exclusive licence to Biomedical Engineering Society. FAU - Zhai, Hannah AU - Zhai H AD - Department of Mechanical Engineering, Columbia University, New York, NY, 10027, USA. FAU - Chen, Yurui AU - Chen Y AD - Department of Mechanical Engineering, Columbia University, New York, NY, 10027, USA. FAU - Kitada, Yuichiro AU - Kitada Y AD - Division of Cardiothoracic and Vascular Surgery, Department of Surgery, New York Presbyterian Hospital/Columbia University Irving Medical Center, New York, NY, 10032, USA. FAU - Takayama, Hiroo AU - Takayama H AD - Division of Cardiothoracic and Vascular Surgery, Department of Surgery, New York Presbyterian Hospital/Columbia University Irving Medical Center, New York, NY, 10032, USA. FAU - Vedula, Vijay AU - Vedula V AUID- ORCID: 0000-0002-3569-3340 AD - Department of Mechanical Engineering, Columbia University, New York, NY, 10027, USA. vv2316@columbia.edu. LA - eng GR - SIRS Blavatnik Fund/Fu Foundation School of Engineering and Applied Science/ PT - Journal Article DEP - 20261027 PL - United States TA - Ann Biomed Eng JT - Annals of biomedical engineering JID - 0361512 SB - IM OTO - NOTNLM OT - Aortic root OT - Aortic valve OT - Ascending thoracic aortic aneurysm OT - Fluid-structure interaction OT - Hemodynamics OT - Patient-specific modeling OT - Sino-tubular junction COIS- Declarations. Conflict of interest: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Ethical Approval: This single-center study was performed following HIPAA-compliant processes and guidelines and was approved by the Columbia University Medical Center Institutional Review Board (IRB#AAAR2949). Consent for Publication: All authors have agreed with the content and given explicit consent to submit the manuscript for publication. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:20 PHST- 2026/01/28 00:00 [received] PHST- 2026/09/07 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:20 [entrez] AID - 10.1007/s10439-026-04375-4 [pii] AID - 10.1007/s10439-026-04375-4 [doi] PST - aheadofprint SO - Ann Biomed Eng. 2026 Oct 27. doi: 10.1007/s10439-026-04375-4. PMID- 42944970 OWN - NLM STAT- Publisher LR - 20261027 IS - 1573-9686 (Electronic) IS - 0090-6964 (Linking) DP - 2026 Oct 27 TI - Predicting Post-TEVAR Endoleaks: A Pre-operative Hemodynamic Risk Factor from Patient-Specific Fluid-Structure Interaction Simulations. LID - 10.1007/s10439-026-04337-w [doi] AB - PURPOSE: Thoracic endovascular aortic repair (TEVAR) is a minimally invasive treatment for thoracic aortic pathologies, including thoracic aortic aneurysm (TAA). Computational simulations can provide valuable insights into TEVAR outcomes and complications before intervention. This proof-of-concept study proposes a novel risk factor R as a potential predictor of post-TEVAR type I and III endoleaks (ELs) using pre-TEVAR hemodynamic data. METHODS: Fluid-structure interaction (FSI) computational simulations are performed in ten pre-TEVAR patient-specific TAA cases, with known follow-up TEVAR outcomes. Hemodynamic drag forces acting on the aortic wall are quantified and used to define R, which combines parallel and perpendicular drag force components with aortic tortuosity. The patient cohort is divided into a calibration set, used to associate specific R values with three different risk levels, and a test set, used to preliminarily assess the agreement between the predictions derived from R and the post-TEVAR follow-up outcomes. RESULTS: Based on the R values obtained for the calibration set, R 0.67 with high risk. Once it is applied to the test set, the risk factor is able to correctly identify a type I EL. CONCLUSION: The risk factor proposed in this work is capable of identifying all the EL cases analyzed. This study represents a preliminary attempt to determine whether pre-TEVAR hemodynamics can effectively predict post-TEVAR complications and therefore aid clinicians in the pre-operative planning. CI - (c) 2026. The Author(s). FAU - Duca, Francesca AU - Duca F AUID- ORCID: 0009-0000-1993-0409 AD - LaBS, Dipatimento di Chimica, Materiali e Ingegneria Chimica 'Giulio Natta', Politecnico di Milano, Piazza Leonardo Da Vinci 32, Milan, 20133, Italy. francesca.duca@polimi.it. FAU - Tavarone, Silvia AU - Tavarone S AD - LaBS, Dipatimento di Chimica, Materiali e Ingegneria Chimica 'Giulio Natta', Politecnico di Milano, Piazza Leonardo Da Vinci 32, Milan, 20133, Italy. FAU - Domanin, Maurizio AU - Domanin M AUID- ORCID: 0000-0001-7510-3520 AD - Department of Clinical Sciences and Community Health, Dipartimento di Eccellenza 2023-2027, Universita degli Studi di Milano, Via Festa del Perdono 7, Milan, 20122, Italy. AD - Section of Vascular Surgery, Cardio Thoracic Vascular Department, Fondazione I.R.C.C.S. Ca' Granda Ospedale Maggiore Policlinico, Via Francesco Sforza 35, Milan, 20122, Italy. FAU - Bissacco, Daniele AU - Bissacco D AUID- ORCID: 0000-0003-0724-0237 AD - Department of Clinical Sciences and Community Health, Dipartimento di Eccellenza 2023-2027, Universita degli Studi di Milano, Via Festa del Perdono 7, Milan, 20122, Italy. AD - Section of Vascular Surgery, Cardio Thoracic Vascular Department, Fondazione I.R.C.C.S. Ca' Granda Ospedale Maggiore Policlinico, Via Francesco Sforza 35, Milan, 20122, Italy. FAU - Trimarchi, Santi AU - Trimarchi S AUID- ORCID: 0000-0001-5996-3264 AD - Department of Clinical Sciences and Community Health, Dipartimento di Eccellenza 2023-2027, Universita degli Studi di Milano, Via Festa del Perdono 7, Milan, 20122, Italy. AD - Section of Vascular Surgery, Cardio Thoracic Vascular Department, Fondazione I.R.C.C.S. Ca' Granda Ospedale Maggiore Policlinico, Via Francesco Sforza 35, Milan, 20122, Italy. FAU - Vergara, Christian AU - Vergara C AUID- ORCID: 0000-0001-9872-5410 AD - LaBS, Dipatimento di Chimica, Materiali e Ingegneria Chimica 'Giulio Natta', Politecnico di Milano, Piazza Leonardo Da Vinci 32, Milan, 20133, Italy. FAU - Migliavacca, Francesco AU - Migliavacca F AUID- ORCID: 0000-0003-4644-630X AD - LaBS, Dipatimento di Chimica, Materiali e Ingegneria Chimica 'Giulio Natta', Politecnico di Milano, Piazza Leonardo Da Vinci 32, Milan, 20133, Italy. AD - Section of Vascular Surgery, Cardio Thoracic Vascular Department, Fondazione I.R.C.C.S. Ca' Granda Ospedale Maggiore Policlinico, Via Francesco Sforza 35, Milan, 20122, Italy. LA - eng GR - 2022L3JC5T/Italian Ministry of Education, Universities and Research (MIUR)/ PT - Journal Article DEP - 20261027 PL - United States TA - Ann Biomed Eng JT - Annals of biomedical engineering JID - 0361512 SB - IM OTO - NOTNLM OT - Endoleak OT - Fluid-structure interaction simulations OT - TEVAR OT - Thoracic aortic aneurysm COIS- Declarations. Competing Interests: No conflict of interest, financial or otherwise, are declared by the authors. Ethical Approval: Informed consent was obtained from each patient included in this study. Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution, and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ . EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:20 PHST- 2026/03/25 00:00 [received] PHST- 2026/08/03 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:20 [entrez] AID - 10.1007/s10439-026-04337-w [pii] AID - 10.1007/s10439-026-04337-w [doi] PST - aheadofprint SO - Ann Biomed Eng. 2026 Oct 27. doi: 10.1007/s10439-026-04337-w. PMID- 42944969 OWN - NLM STAT- Publisher LR - 20261027 IS - 2948-2933 (Electronic) IS - 2948-2925 (Linking) DP - 2026 Oct 27 TI - A Combined Clinical, Spectral CT, and Intratumoral Radiomics Model for Predicting Lymph Node Metastasis in Gastric Adenocarcinoma. LID - 10.1007/s10278-026-02466-1 [doi] AB - Lymph node metastasis (LNM) significantly impacts gastric adenocarcinoma (GA) treatment and prognosis. Preoperative LNM prediction is challenging, necessitating accurate models to assist clinical decisions. We aim to establish and validate a model integrating multiparameter dual-layer detector spectral CT (DLCT) imaging features and radiomics scores from venous phase images for preoperative LNM prediction in GA patients. We retrospectively analyzed clinical, pathological, and imaging data from 275 GA patients with preoperative DLCT scans, categorized into LNM-positive (173 cases) and LNM-negative (102 cases) groups. Intra- and peritumoral DLCT metrics, including iodine concentration (IC), normalized IC (nIC), and effective atomic number (Zeff), were assessed. Independent risk factors were identified via logistic regression. Radiomics features were extracted from venous phase DLCT images, with the least absolute shrinkage and selection operator (LASSO) used for radiomics feature selection. A combined model incorporating clinical data, DLCT metrics, and radiomics scores was constructed and evaluated using receiver operating characteristic (ROC) curves, calibration curves, and decision curve analysis (DCA). Intratumoral venous phase nIC and carbohydrate antigen 19-9 (CA 19-9) were independent risk factors, forming a conventional model. Five radiomics features were selected for a radiomics model. The combined (CA 19-9 + venous phase + radiomics scores) model showed superior predictive performance, with AUCs of 0.91 (training set) and 0.83 (validation set). Calibration curves indicated a good fit, and DCA demonstrated a favorable net benefit across relevant threshold probabilities for the combined model. The combined model, which integrates clinical information with both multiparameter DLCT features and radiomics scores, offers a clinically valuable tool for preoperative LNM prediction in GA patients. CI - (c) 2026. The Author(s) under exclusive licence to Society for Imaging Informatics in Medicine. FAU - Wang, Dongfang AU - Wang D AD - Department of Radiology, Xiamen Radiology Quality Control Center, Zhongshan Hospital Xiamen University, School of Medicine, Xiamen University, Xiamen, 361004, Fujian, China. AD - Department of Radiology, Hebei Yanda Hospital, Langfang, 065201, China. FAU - Lin, Yu AU - Lin Y AD - Department of Radiology, Xiamen Radiology Quality Control Center, Zhongshan Hospital Xiamen University, School of Medicine, Xiamen University, Xiamen, 361004, Fujian, China. AD - Xiamen Key Laboratory of Nuclear Medicine and Molecular Imaging for Clinical Translation, Xiamen University, Xiamen, 361004, Fujian, China. FAU - Lin, Zeyang AU - Lin Z AD - Department of Pathology, Zhongshan Hospital Xiamen University, School of Medicine, Xiamen University, Xiamen, 361004, Fujian, China. FAU - Hong, Yanling AU - Hong Y AD - Department of Radiology, Xiamen Radiology Quality Control Center, Zhongshan Hospital Xiamen University, School of Medicine, Xiamen University, Xiamen, 361004, Fujian, China. FAU - Wei, Shunjie AU - Wei S AD - Department of Radiology, Xiamen Radiology Quality Control Center, Zhongshan Hospital Xiamen University, School of Medicine, Xiamen University, Xiamen, 361004, Fujian, China. FAU - Gan, Shufen AU - Gan S AD - Department of Ultrasound Medicine, Zhongshan Hospital Xiamen University, School of Medicine, Xiamen University, Xiamen, 361004, Fujian, China. heartliverbabi@163.com. FAU - Zeng, Qiang AU - Zeng Q AUID- ORCID: 0009-0007-8445-0285 AD - Department of Radiology, Xiamen Radiology Quality Control Center, Zhongshan Hospital Xiamen University, School of Medicine, Xiamen University, Xiamen, 361004, Fujian, China. qianglf@xmu.edu.cn. AD - Xiamen Key Laboratory of Nuclear Medicine and Molecular Imaging for Clinical Translation, Xiamen University, Xiamen, 361004, Fujian, China. qianglf@xmu.edu.cn. LA - eng GR - 2020J011216/Natural Science Foundation of Fujian Province/ GR - 2024J011337/Natural Science Foundation of Fujian Province/ GR - 2026J0011711/Natural Science Foundation of Fujian Province/ GR - 2022J011343/Natural Science Foundation of Fujian Province/ GR - 3502Z20234007/Key Medical and Health Projects of Xiamen Municipality/ GR - 3502Z20214ZD1032/Natural Science Foundation of Xiamen Municipality/ PT - Journal Article DEP - 20261027 PL - Switzerland TA - J Imaging Inform Med JT - Journal of imaging informatics in medicine JID - 9918663679206676 SB - IM OTO - NOTNLM OT - Dual-layer detector spectral CT OT - Gastric adenocarcinoma OT - Lymph node metastasis OT - Preoperative prediction OT - Radiomics COIS- Declarations. Ethics Approval and Consent to Participate: This study received approval from the Ethics Committee of Zhongshan Hospital Xiamen University (approval number 2026-072). Written informed consent was obtained from all individual participants after they were thoroughly informed about the study's objectives, methodologies, potential risks, and benefits. The informed consent process adhered to the principles outlined in the Declaration of Helsinki and complied with applicable national regulations. All participants voluntarily consented to participate, with full awareness that their data would be utilized for scientific analysis. Consent for Publication: Not applicable. This manuscript does not contain any individual person's data, images, or clinical details that would compromise anonymity, and therefore, no specific consent for publication is required. Conflict of Interest: No potential conflict of interest relevant to this article was reported. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:20 PHST- 2026/08/25 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:20 [entrez] AID - 10.1007/s10278-026-02466-1 [pii] AID - 10.1007/s10278-026-02466-1 [doi] PST - aheadofprint SO - J Imaging Inform Med. 2026 Oct 27. doi: 10.1007/s10278-026-02466-1. PMID- 42944964 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 1750-0680 (Print) IS - 1750-0680 (Electronic) IS - 1750-0680 (Linking) VI - 21 IP - 1 DP - 2026 Sep 21 TI - Quantifying carbon benefits from protecting and restoring tropical forest landuse mosaics in Vanuatu. LID - 10.1186/s13021-026-00505-6 [doi] LID - 129 AB - BACKGROUND: Forest loss and degradation in Vanuatu is a pertinent environmental issue, particularly for rural communities who are inherently reliant on subsistence-based agriculture derived from tropical forest landuse mosaics. Payment for Ecosystem Services (PES) projects provide a mechanism to diversify agricultural livelihoods while reducing deforestation and forest degradation and promoting ecosystem restoration. This study evaluates the Life & Land Project, a PES initiative working with communities at Whitegrass and Middlebush on Tanna, and South River on Erromango. Few primary studies in Vanuatu have quantified forest carbon stocks or evaluated changes in carbon stocks resulting from PES interventions. RESEARCH OBJECTIVES AND METHODS: These knowledge gaps are addressed by assessing: (a) forest carbon stocks and structural characteristics; (b) the drivers and rates of deforestation and forest degradation, together with pressures affecting forest health, to inform management interventions; and (c) the potential carbon benefits of implementing these interventions using a gain-loss approach. RESULTS: Results showed high variability in structural composition and carbon stocks, reflective of differences in landuse and environmental pressures. The mature, predominantly primary tropical rainforest at Whitegrass had an average carbon stock of 490.7 +/- 32.6 Mg C ha(-1), in contrast to the invasive vine-smothered degraded forests of Imanaka Creek (Middlebush) and the Forest Enhancement Zone of South River (107.3 +/- 10.7 Mg C ha(-1) and 128.2 +/- 22.7 Mg C ha(-1) respectively). Forest loss at Whitegrass was driven through logging, ad-hoc garden development, and quarry operations at an estimated rate of 3.55 ha(-1) year(-1), while invasive vines were the main impediment impacting forest health at most sites. To address pressures, a conservation area at Whitegrass (Forest Protection) was established with community developed and enforced by-laws, while Forest Restoration and Improved Forest Management interventions involved undertaking vine clearance, maintenance, and replanting with a mix of agro-forestry and native species (all sites). Overall carbon benefits are estimated at 6,688 Mg CO(2)e year(-1). CONCLUSIONS: The study helps Vanuatu and other Small Island Developing States, particularly those of the South Pacific, strengthen the platform from which other PES projects could be developed and implemented. CI - (c) 2026. The Author(s). FAU - Cameron, Clint AU - Cameron C AD - Moana Conservation, 9a Hayley Grove, Papamoa Beach, New Zealand. clintcameron1@yahoo.co.nz. FAU - Bird, Olivia AU - Bird O AD - Tearfund New Zealand, 64 C Stoddard Road, Mount Roskill, Auckland, 1041, New Zealand. FAU - Lava, Jeffery AU - Lava J AD - Nasituan, Imanaka Road, Tanna, Vanuatu. Jeffrey@nasituan.org. FAU - Finaly, Andrew AU - Finaly A AD - Tearfund New Zealand, 64 C Stoddard Road, Mount Roskill, Auckland, 1041, New Zealand. LA - eng PT - Journal Article DEP - 20260921 PL - England TA - Carbon Balance Manag JT - Carbon balance and management JID - 101271519 PMC - PMC13711963 OTO - NOTNLM OT - Carbon stocks OT - Community structure OT - Landuse and landcover change OT - Tropical rainforests OT - Vanuatu COIS- Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The Authors declare that there were no competing interests associated with the research, authorship, or publication of this study. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/09/21 CRDT- 2026/10/27 12:19 PHST- 2026/02/13 00:00 [received] PHST- 2026/08/19 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:19 [entrez] PHST- 2026/09/21 00:00 [pmc-release] AID - 10.1186/s13021-026-00505-6 [pii] AID - 505 [pii] AID - 10.1186/s13021-026-00505-6 [doi] PST - epublish SO - Carbon Balance Manag. 2026 Sep 21;21(1):129. doi: 10.1186/s13021-026-00505-6. PMID- 42944962 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1546-9549 (Electronic) IS - 1546-9530 (Print) IS - 1546-9530 (Linking) VI - 23 IP - 1 DP - 2026 Oct 27 TI - Don't Wait- Ablate! Catheter Ablation in Patients with Advanced Heart Failure and Atrial Fibrillation. LID - 10.1007/s11897-026-00787-4 [doi] LID - 52 AB - PURPOSE OF REVIEW: Heart failure (HF) and atrial fibrillation (AF) frequently coexist and mutually accelerate disease progression through complex hemodynamic, neurohormonal, and structural interactions. Although earlier rhythm-control strategies failed to demonstrate mortality benefit, largely due to limitations of antiarrhythmic drug therapy, catheter ablation has fundamentally altered the therapeutic landscape. RECENT FINDINGS: The CASTLE-AF trial established catheter ablation as a disease-modifying therapy in patients with AF and HF with reduced ejection fraction (HFrEF), demonstrating reductions in mortality and HF hospitalization. More recently, the CASTLE-HTx trial extended these findings to patients with end-stage HF eligible for heart transplantation (HTx) or left ventricular assist device (LVAD) therapy. Catheter ablation has demonstrated a marked reduction in mortality and the need of an urgent HTx, or LVAD implantation compared with medical therapy alone. Subsequent subanalyses further refined risk stratification and identified patient subsets deriving the greatest benefit. Therefore, catheter ablation has received a class 1 recommendation for patients with AF and HFrEF. According to recent clinical evidence supporting early rhythm control through catheter ablation in advanced HF, it leads to a paradigm shift in the management of this high-risk population: in selected patients with AF and advanced HF, clinicians should not delay referral-"Don't wait, ablate." CI - (c) 2026. The Author(s). FAU - Moersdorf, Maximilian AU - Moersdorf M AD - Department of Electrophysiology, Heart- and Diabetescenter NRW, Ruhr-University Bochum, Bad Oeynhausen, Germany. AD - Tulane Research Innovation for Arrhythmia Discovery (TRIAD), Tulane University, New Orleans, LA, USA. FAU - Sommer, Philipp AU - Sommer P AD - Department of Electrophysiology, Heart- and Diabetescenter NRW, Ruhr-University Bochum, Bad Oeynhausen, Germany. AD - Department of Electrophysiology, Heart- and Diabetescenter NRW, University Bielefeld, Medical Faculty OWL, Bad Oeynhausen, Germany. FAU - Sohns, Christian AU - Sohns C AD - Department of Electrophysiology, Heart- and Diabetescenter NRW, Ruhr-University Bochum, Bad Oeynhausen, Germany. csohns@hdz-nrw.de. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - United States TA - Curr Heart Fail Rep JT - Current heart failure reports JID - 101196487 SB - IM MH - Humans MH - *Atrial Fibrillation/surgery/complications/physiopathology MH - *Heart Failure/complications/surgery/physiopathology MH - *Catheter Ablation/methods MH - Heart Transplantation MH - Stroke Volume/physiology MH - Heart-Assist Devices PMC - PMC13714006 OTO - NOTNLM OT - Advanced heart failure OT - Atrial fibrillation OT - Catheter ablation OT - Heart transplantation OT - Mortality COIS- Declarations. Conflict of interest: MM received a research grant from the German Heart Foundation. PS is a member of the advisory board of Abbott, Boston Scientific, J&J MedTech and Medtronic. CS reports having received lecture fees and is a consultant for J&J MedTech., Boston Scientific, and Medtronic. The authors have no other conflicts of interest to disclose. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 12:19 PHST- 2026/05/15 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:19 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 10.1007/s11897-026-00787-4 [pii] AID - 787 [pii] AID - 10.1007/s11897-026-00787-4 [doi] PST - epublish SO - Curr Heart Fail Rep. 2026 Oct 27;23(1):52. doi: 10.1007/s11897-026-00787-4. PMID- 42944961 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 2731-9229 (Electronic) IS - 2731-9229 (Linking) VI - 21 IP - 1 DP - 2026 Oct 27 TI - Innovations in engineered nanomaterials for enhanced CO(2) adsorption and climate change mitigation. LID - 10.1186/s11671-026-05010-w [doi] LID - 565 AB - Climate change, characterized by persistent alterations in weather patterns from tropical to polar regions, poses a multifaceted challenge that affects numerous sectors globally. Moreover, climate change is also endangering the existence and integrity of many species by disrupting their optimal temperature ranges, thus exacerbating the loss of biodiversity by reshaping ecosystems. The emergence and spread of diseases, exemplified by the recent coronavirus pandemic, are also influenced by climate variability, which enhances the likelihood of foodborne, waterborne, and vector-borne illnesses. The accelerating rise in atmospheric carbon dioxide (CO(2)) concentrations is a major driver of global climate change, necessitating the development of effective mitigation technologies. Among the emerging solutions, engineered nanomaterials (ENMs) have demonstrated exceptional potential for CO(2) capture owing to their tunable surface chemistry, high porosity, and superior adsorption capacity. This review systematically examines recent innovations in nanostructured materials-such as metal-organic frameworks (MOFs), carbon-based nanomaterials, zeolitic materials, and hybrid composites-designed to enhance CO(2) adsorption performance. The underlying adsorption mechanisms, including physisorption, chemisorption, and surface functionalization strategies, are discussed in detail to elucidate structure-property relationships. Special attention is given to the synergistic effects of nanoparticle modification, doping, and heterostructure engineering in optimizing adsorption kinetics and selectivity. Furthermore, the review evaluates experimental and computational advancements that bridge the gap between laboratory-scale findings and industrial implementation. The study highlights how the rational design of ENMs contributes not only to CO(2) capture efficiency but also to the broader goal of sustainable carbon management and climate change mitigation. Finally, future perspectives on scalable synthesis, environmental safety, and integration with renewable energy systems are outlined to guide forthcoming research directions. CI - (c) 2026. The Author(s). FAU - Alsehli, Amal H AU - Alsehli AH AD - Department of Chemistry, College of Science, Taibah University, 3002, Al-Madinah Al Munawarah, Saudi Arabia. FAU - A Eskander, Thomas Nady AU - A Eskander TN AD - Department of Chemistry, Faculty of Science, Sohag University, Sohag, 82524, Egypt. FAU - Alahmadi, Sana M AU - Alahmadi SM AD - Department of Chemistry, College of Science, Taibah University, 3002, Al-Madinah Al Munawarah, Saudi Arabia. FAU - Khashoqji, Moayad M AU - Khashoqji MM AD - Applied College, Taibah University, Madinah, 42353, Saudi Arabia. FAU - Al-Farraj, Eida S AU - Al-Farraj ES AD - Department of Chemistry, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU), Riyadh, 11623, Saudi Arabia. FAU - Fathalla, Maher AU - Fathalla M AD - Department of Chemistry, Faculty of Science, Islamic University of Madinah, Madinah, 42351, Saudi Arabia. FAU - Abd El-Lateef, Hany M AU - Abd El-Lateef HM AD - Department of Chemistry, College of Science, King Faisal University, Al-Ahsa, 31982, Saudi Arabia. hmahmed@kfu.edu.sa. FAU - Elhady, Omar AU - Elhady O AD - Department of Chemistry, Faculty of Science, Sohag University, Sohag, 82524, Egypt. FAU - Abu-Dief, Ahmed M AU - Abu-Dief AM AD - Department of Chemistry, College of Science, Taibah University, 3002, Al-Madinah Al Munawarah, Saudi Arabia. amamohammed@taibahu.edu.sa. AD - Department of Chemistry, Faculty of Science, Sohag University, Sohag, 82524, Egypt. amamohammed@taibahu.edu.sa. LA - eng GR - KFU263289/King Faisal University/ PT - Journal Article PT - Review DEP - 20261027 PL - Switzerland TA - Discov Nano JT - Discover nano JID - 9918540788706676 PMC - PMC13713970 OTO - NOTNLM OT - Adsorption mechanisms OT - Carbon dioxide (CO2) capture OT - Climate change OT - Engineered nanomaterials (ENMs) OT - Metal-organic frameworks (MOFs) OT - Sustainable development COIS- Declarations. Ethical approval: not applicable. Consent to publish: not applicable. Consent to participate: not applicable. Competing interests: The authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 12:19 PHST- 2026/01/16 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:19 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 10.1186/s11671-026-05010-w [pii] AID - 5010 [pii] AID - 10.1186/s11671-026-05010-w [doi] PST - epublish SO - Discov Nano. 2026 Oct 27;21(1):565. doi: 10.1186/s11671-026-05010-w. PMID- 42944956 OWN - NLM STAT- Publisher LR - 20261027 IS - 1573-4994 (Electronic) IS - 1053-0509 (Linking) DP - 2026 Oct 27 TI - From Acidochromism to Device Fabrication: A DFT-Backed Chemosensor for Portable Co(2)(+) and Cu(2)(+) Sensing. LID - 10.1007/s10895-026-04981-4 [doi] AB - For the purpose of selectively detecting Co(2+) and Cu(2+) ions using a combination of colorimetric and fluorometric responses, a novel coumarin-imidazole-thiazole based chemosensor 6-chloro-3-(2-(2-(4-(diphenylamino)phenyl)-1H-phenanthro[9,10-d]imidazol-1-yl)thiazol-4-yl)-2H-chromen-2-one (TDC2) was created and thoroughly studied. When TDC2 interacts with Co(2)(+) and Cu(2)(+), it shows noticeable colour changes, the colour changes from mild yellow to sea blue for Cu(2)(+) and in presence of Co(2+) ions colour changes to baby pink along with strong fluorescence quenching, but it is insensitive to other competing metal ions. Excellent linearity and high sensitivity are demonstrated by UV-visible and fluorescence titration tests, with nanomolar limits of detection of 45 nM for Co(2)(+) and 68 nM for Cu(2)(+). The sensor exhibits reversible acidochromic behaviour toward volatile acid vapours and functions well across a broad pH range. Polymer-embedded films and paper-strip devices were developed to convert molecular sensing into useful platforms. These allowed for quick, portable, on-site detection with distinct visual responses under both visible and UV light. TDC2 is positioned as a reliable and application-focused sensor for real-time monitoring of Co(2+) and Cu(2+) ions due to its combination solution-state performance, solid-state device integration, and theoretical validation. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Nagalik, Jyoti AU - Nagalik J AD - Laser Spectroscopy Programme, Department of Physics, Karnatak University, Dharwad, 580003, Karnataka, India. FAU - Nesaragi, Aravind R AU - Nesaragi AR AD - Department of Chemistry, RNS Institute of Technology, Bangalore, 560098, Karnataka, India. nesaragiaravind@gmail.com. FAU - T M, Sharanakumar AU - T M S AD - Department of Chemistry, Ballari Institute of Technology and Management, Ballari, 583104, Karnataka, India. FAU - Iqbal, Muzaffar AU - Iqbal M AD - Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box: 2457, Riyadh, 11451, Saudi Arabia. FAU - Bansal, Nisha AU - Bansal N AD - Department of Biotechnology, Graphic Era Deemed to be University, Dehradun, 248002, Uttarakhand, India. FAU - Singh, Thakur Gurjeet AU - Singh TG AD - Centre of Research Impact and Outcome, Chitkara College of Pharmacy, Chitkara University, Rajpura, 140401, Punjab, India. FAU - Verma, Akash AU - Verma A AD - Teerthanker Mahaveer College of Pharmacy, Teerthanker Mahaveer University, Moradabad, 244001, Uttar Pradesh, India. FAU - Majumder, Poulami AU - Majumder P AD - Department of Chemistry for Life Sciences, Uppsala University, Uppsala, 75123, Sweden. FAU - Imam, Faisal AU - Imam F AD - Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box: 2457, Riyadh, 11451, Saudi Arabia. FAU - Inamdar, Sanjeev R AU - Inamdar SR AD - Laser Spectroscopy Programme, Department of Physics, Karnatak University, Dharwad, 580003, Karnataka, India. LA - eng GR - ORF-2026-734/King Saud University/ PT - Journal Article DEP - 20261027 PL - Netherlands TA - J Fluoresc JT - Journal of fluorescence JID - 9201341 SB - IM OTO - NOTNLM OT - Acidochromism OT - Colorimetric and fluorometric sensing OT - Co(2)(+) and Cu(2)(+) detection OT - DFT studies OT - Device fabrication COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:19 PHST- 2026/07/29 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:19 [entrez] AID - 10.1007/s10895-026-04981-4 [pii] AID - 10.1007/s10895-026-04981-4 [doi] PST - aheadofprint SO - J Fluoresc. 2026 Oct 27. doi: 10.1007/s10895-026-04981-4. PMID- 42944955 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261027 IS - 0948-5023 (Electronic) IS - 0948-5023 (Linking) VI - 32 IP - 11 DP - 2026 Oct 27 TI - Alkaline-earth substitution effects on the physical properties and hydrogen-storage performance of cubic MSc(3)H(8) (M = Ba, Ca, Sr) hydrides: a first-principles study. LID - 447 [pii] LID - 10.1007/s00894-026-07036-4 [doi] AB - CONTEXT: First-principles calculations were employed to investigate the effect of alkaline-earth substitution on the physical properties and hydrogen-storage performance of cubic MSc(3)H(8) (M = Ba, Ca, Sr) hydrides. All three compounds exhibit negative formation energies relative to the selected elemental reference states and satisfy the mechanical-stability criteria. Their calculated elastic indicators suggest a predominantly brittle response, with CaSc(3)H(8) exhibiting the highest overall stiffness and BaSc(3)H(8) the lowest elastic anisotropy. The electronic structures are metallic, with Sc-3d states dominating near the Fermi level and appreciable Sc-3d/H-1s hybridization in the valence region. Phonon calculations indicate local harmonic dynamical stability of the optimized cubic phases at 0 K. Among the investigated compounds, CaSc3H8 exhibits both the highest gravimetric hydrogen capacity (4.41 wt%) and the highest volumetric hydrogen density (112.80 g H(2) L(-1)), whereas BaSc(3)H(8) has the lowest estimated desorption temperature 340 K, indicating comparatively easier hydrogen release within the simplified thermodynamic model adopted in this work. METHODS: Density functional theory calculations were performed using the Quantum ESPRESSO package with the PBE generalized-gradient approximation and PAW pseudopotentials. Plane-wave kinetic-energy cutoffs of 60 Ry and 600 Ry were used for the wave functions and charge density, respectively, together with an 8x8x8 k-point mesh. Lattice-dynamical properties were evaluated within density-functional perturbation theory using a 4x4x4 q-point mesh, while phonon densities of states were obtained using an 8x8x8 q-point mesh. Vibrational thermodynamic properties were evaluated using the thermo_pw package. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Sabir, Kaoutar AU - Sabir K AD - LPHE-MS, Science Faculty, Mohammed V University in Rabat, Rabat, Morocco. FAU - Ouahab, Nabil AU - Ouahab N AD - LPHE-MS, Science Faculty, Mohammed V University in Rabat, Rabat, Morocco. nabil.ouahab@um5r.ac.ma. FAU - El Fatni, Omkaltoume AU - El Fatni O AD - LPHE-MS, Science Faculty, Mohammed V University in Rabat, Rabat, Morocco. LA - eng PT - Journal Article DEP - 20261027 PL - Germany TA - J Mol Model JT - Journal of molecular modeling JID - 9806569 SB - IM OTO - NOTNLM OT - Density functional theory OT - Electronic structure OT - Hydrogen storage OT - Lattice dynamics OT - Mechanical properties OT - Sc-based hydrides COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:19 PHST- 2026/09/13 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:19 [entrez] AID - 10.1007/s00894-026-07036-4 [pii] AID - 10.1007/s00894-026-07036-4 [doi] PST - epublish SO - J Mol Model. 2026 Oct 27;32(11):447. doi: 10.1007/s00894-026-07036-4. PMID- 42944947 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1432-1939 (Electronic) IS - 0029-8549 (Linking) VI - 208 IP - 11 DP - 2026 Oct 27 TI - Reduced habitat connectivity drives gigantism in an amphibian species within the largest archipelago of China. LID - 159 [pii] LID - 10.1007/s00442-026-06001-5 [doi] AB - The island rule predicts that small-bodied species evolve larger sizes on islands, whereas large-bodied species become smaller. Despite widespread attention, tests in amphibians have yielded conflicting results. Moreover, previous work has largely focused on body size, leaving how other morphological traits respond to insular conditions unclear. Here we address these questions by testing the island rule in the ornate narrow-mouthed frog (Microhyla fissipes) in a land-bridge system by measuring seven morphological traits and quantifying island- and site-level environmental variables. After controlling for age, island populations exhibited significantly larger snout-vent length and body mass than their mainland counterparts, supporting classic island gigantism. Head width and length also increased on islands, whereas limb lengths did not differ significantly. Multivariate models revealed that, at the island level, habitat connectivity was consistently negatively associated with body length and mass, while humidity and wind speed showed positive effects. Moreover, head width responded negatively to predator richness, and limb lengths increased with conspecific density. Notably, mainland sites showed body size gigantism driven by low habitat connectivity, indicating that the same pattern can arise in functionally similar habitats. Overall, we emphasize that immigrant selection driven by reduced habitat connectivity, together with microhabitat variation, shapes body size gigantism. Additionally, predator release and intraspecific competition influence head and limb morphology, respectively. We highlight the importance of incorporating habitat connectivity and a multidimensional trait approach into island rule research. Our study also warns that mainland habitats are undergoing progressive islandization due to fragmentation and reduced connectivity. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Lu, Yuxin AU - Lu Y AD - Laboratory of Island Biogeography and Conservation Biology, College of Life Sciences, Nanjing Normal University, Nanjing, China. FAU - Jiang, Xu AU - Jiang X AD - Laboratory of Island Biogeography and Conservation Biology, College of Life Sciences, Nanjing Normal University, Nanjing, China. FAU - Huang, Yiyao AU - Huang Y AD - Laboratory of Island Biogeography and Conservation Biology, College of Life Sciences, Nanjing Normal University, Nanjing, China. FAU - Hao, Jiajun AU - Hao J AD - College of Ecology, Lishui University, Lishui, Zhejiang, China. FAU - Song, Jiawei AU - Song J AD - Laboratory of Island Biogeography and Conservation Biology, College of Life Sciences, Nanjing Normal University, Nanjing, China. FAU - Wang, Suchen AU - Wang S AD - Laboratory of Island Biogeography and Conservation Biology, College of Life Sciences, Nanjing Normal University, Nanjing, China. FAU - Zhan, Chengxiu AU - Zhan C AD - Laboratory of Island Biogeography and Conservation Biology, College of Life Sciences, Nanjing Normal University, Nanjing, China. FAU - Zhang, Caiwen AU - Zhang C AD - School of Life Sciences and Medical Engineering, Anhui University, Hefei, Anhui, China. FAU - Ding, Guohua AU - Ding G AUID- ORCID: 0000-0002-2484-5855 AD - College of Ecology, Lishui University, Lishui, Zhejiang, China. guwoding@lsu.edu.cn. FAU - Wang, Yanping AU - Wang Y AUID- ORCID: 0000-0003-3743-3937 AD - Laboratory of Island Biogeography and Conservation Biology, College of Life Sciences, Nanjing Normal University, Nanjing, China. wangyanping@njnu.edu.cn. FAU - Chen, Chuanwu AU - Chen C AUID- ORCID: 0000-0002-3974-853X AD - Laboratory of Island Biogeography and Conservation Biology, College of Life Sciences, Nanjing Normal University, Nanjing, China. chencw@nnu.edu.cn. LA - eng GR - 32471601/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261027 PL - Germany TA - Oecologia JT - Oecologia JID - 0150372 SB - IM MH - Animals MH - *Ecosystem MH - China MH - Body Size MH - Islands MH - *Anura MH - *Gigantism OTO - NOTNLM OT - Microhyla fissipes OT - Habitat connectivity OT - Island rule OT - Morphological traits COIS- Declarations. Consent for publication: Not applicable. Conflict of interest: The authors declare no competing interests. Ethics approval: All animal procedures were approved by the Animal Experimental Ethical Inspection Form of Nanjing Normal University (IACUC-2074207). Consent to participate: Not applicable. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:18 PHST- 2026/04/20 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:18 [entrez] AID - 10.1007/s00442-026-06001-5 [pii] AID - 10.1007/s00442-026-06001-5 [doi] PST - epublish SO - Oecologia. 2026 Oct 27;208(11):159. doi: 10.1007/s00442-026-06001-5. PMID- 42944945 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1436-5073 (Electronic) IS - 0026-3672 (Linking) VI - 193 IP - 11 DP - 2026 Oct 27 TI - A multi-enzyme cascade catalytic system constructed on MIL-88B(Fe)-NH(2) as a three-in-one fluorescent nanozyme for multimodal detection of alpha-amylase. LID - 763 [pii] LID - 10.1007/s00604-026-08465-x [doi] AB - A multi‑enzyme cascade sensing platform for alpha‑amylase detection is reported constructed by integrating glucose oxidase (GOx), glucoamylase (Glu), and a peroxidase‑mimicking metal-organic framework (NH(2)‑MIL‑88B) into a cascade catalytic system. In this design, alpha‑amylase hydrolyzes starch to generate maltose, which is catalyzed sequentially by Glu and GOx to produce H(2)O(2), followed by MOF‑mediated chromogenic oxidation to yield a measurable UV‑Vis signal. The generated ox-TMB can quench the fluorescence of NH(2)-MIL-88B and cause a photothermal effect. Consequently, the platform achieved colorimetric/fluorescence/photothermal-based multimodal detection of alpha-amylase. The cascade strategy afforded significant signal amplification, enabling sensitive and selective detection. The system exhibited excellent selectivity against common interferents, including metal ions, small molecules, and proteins. Practical applicability was validated by assaying alpha‑amylase in Daqu. Remarkably, the assay is operationally simple. This work provides a promising solution for alpha‑amylase monitoring in complex samples, with broad prospects in food safety control. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature. FAU - Liu, Huan AU - Liu H AD - Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang, 315211, China. FAU - Wang, Yanlin AU - Wang Y AD - Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang, 315211, China. wangyanlin@cqut.edu.cn. FAU - Wu, Chaoqian AU - Wu C AD - Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang, 315211, China. FAU - Huang, Wanqiu AU - Huang W AD - Guizhou Key Laboratory of Microbial Resources Exploration in Fermentation industry, Kweichow Moutai Group, Zunyi, 564501, China. FAU - Han, Yin AU - Han Y AD - Guizhou Key Laboratory of Microbial Resources Exploration in Fermentation industry, Kweichow Moutai Group, Zunyi, 564501, China. 273376685@qq.com. FAU - Yu, Shaoning AU - Yu S AD - Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang, 315211, China. yushaoning@nbu.edu.cn. LA - eng PT - Journal Article DEP - 20261027 PL - Austria TA - Mikrochim Acta JT - Mikrochimica acta JID - 7808782 RN - EC 3.2.1.1 (alpha-Amylases) RN - EC 1.1.3.4 (Glucose Oxidase) RN - 0 (Metal-Organic Frameworks) RN - EC 3.2.1.3 (Glucan 1,4-alpha-Glucosidase) RN - 0 (Fluorescent Dyes) SB - IM MH - *alpha-Amylases/analysis/metabolism/blood MH - Glucose Oxidase/chemistry/metabolism MH - *Metal-Organic Frameworks/chemistry MH - Glucan 1,4-alpha-Glucosidase/chemistry/metabolism MH - Limit of Detection MH - *Fluorescent Dyes/chemistry MH - Colorimetry/methods MH - Biosensing Techniques/methods MH - Biocatalysis MH - Spectrometry, Fluorescence/methods OTO - NOTNLM OT - MOF OT - Multi-enzyme cascade OT - Multi-modal sensing OT - alpha-Amylase COIS- Declarations. AI disclosure: No artificial intelligence (AI) tools were used in the preparation of this manuscript, including the writing, figures, or graphical abstract. Competing interests: The authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:18 PHST- 2026/07/31 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:18 [entrez] AID - 10.1007/s00604-026-08465-x [pii] AID - 10.1007/s00604-026-08465-x [doi] PST - epublish SO - Mikrochim Acta. 2026 Oct 27;193(11):763. doi: 10.1007/s00604-026-08465-x. PMID- 42944934 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1435-604X (Electronic) IS - 0268-8921 (Print) IS - 0268-8921 (Linking) VI - 41 IP - 1 DP - 2026 Oct 27 TI - Red and near-infrared photobiomodulation in precision medicine: mechanisms, dosimetry, and clinical translation. LID - 10.1007/s10103-026-05077-y [doi] LID - 276 AB - Phototherapy is a non-invasive therapeutic modality that uses specific wavelengths of light to modulate biological processes and treat disease. Driven by advances in photobiology, regenerative medicine, and clinical technology, phototherapy has evolved from empirical heliotherapy into a mechanism-based intervention with expanding medical applications. This review places the biological effects, therapeutic indications, and safety profiles of ultraviolet, blue, and green light in comparative context, while focusing primarily on the mechanisms, dosimetry, and clinical translation of red and near-infrared photobiomodulation (PBM). Although ultraviolet and blue light therapies have established roles in dermatology and neonatal jaundice, their clinical use is limited by concerns such as photocarcinogenesis, retinal phototoxicity, and circadian disruption. In contrast, red and near-infrared light offer deeper tissue penetration and, when appropriately dosed, a comparatively low risk of direct photochemical injury. Through mitochondrial, redox, inflammatory, and other context-dependent signaling pathways, red/NIR PBM has been investigated in wound repair, chronic pain, osteoarthritis, musculoskeletal disorders, neurological conditions, and dermatological applications. As a representative quantitative example, a 2024 meta-analysis of 10 placebo-controlled randomized trials involving 542 participants with knee osteoarthritis found a moderate reduction in pain at rest with PBM compared with placebo (standardized mean difference, - 0.70; 95% confidence interval, - 1.10 to - 0.20), although the certainty of evidence was very low. However, broader clinical translation remains constrained by heterogeneous dosimetry, insufficiently standardized treatment protocols, and uneven evidence maturity across indications. To address these limitations, we propose a precision-oriented framework that links clinical target, tissue-optical context, wavelength selection, patient- and site-specific modifiers, dosimetric implications, measurable endpoints, and evidence maturity. This framework is intended to support treatment planning, parameter reporting, and prospective validation rather than to prescribe currently validated individualized dosing algorithms. CI - (c) 2026. The Author(s). FAU - Shi, Lihao AU - Shi L AUID- ORCID: 0009-0002-7077-4581 AD - Medical Equipment Department, Affiliated Hospital of Jining Medical University, Jining, Shandong, China. FAU - Lu, Jiaqi AU - Lu J AUID- ORCID: 0009-0009-5300-0139 AD - Clinical Medicine College (Affiliated Hospital), Jining Medical University, Jining, China. FAU - Wei, Bin AU - Wei B AD - School of Mechatronic Engineering and Automation, Shanghai University, Shanghai, China. bwei@shu.edu.cn. FAU - Zhou, Pengchao AU - Zhou P AD - Medical Equipment Department, Affiliated Hospital of Jining Medical University, Jining, Shandong, China. pczhou2014@126.com. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - England TA - Lasers Med Sci JT - Lasers in medical science JID - 8611515 SB - IM MH - Humans MH - *Low-Level Light Therapy/methods MH - Red Light MH - *Precision Medicine/methods MH - *Infrared Rays/therapeutic use MH - Blue Light PMC - PMC13714148 OTO - NOTNLM OT - Near-infrared light OT - Photobiomodulation OT - Phototherapy OT - Precision medicine OT - Red light COIS- Declarations. Ethics approval and consent to participate: Ethics statement: not applicable. Competing interests: The authors declare no competing interests. Clinical trial number: Not applicable. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 12:17 PHST- 2026/07/26 00:00 [received] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:17 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 10.1007/s10103-026-05077-y [pii] AID - 5077 [pii] AID - 10.1007/s10103-026-05077-y [doi] PST - epublish SO - Lasers Med Sci. 2026 Oct 27;41(1):276. doi: 10.1007/s10103-026-05077-y. PMID- 42944933 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1573-4978 (Electronic) IS - 0301-4851 (Linking) VI - 53 IP - 1 DP - 2026 Oct 27 TI - Schwann cell reprogramming and immune regulation in the cancer microenvironment. LID - 1837 [pii] LID - 10.1007/s11033-026-13000-5 [doi] AB - Schwann cells are peripheral glia with established roles in nerve support and repair. In cancer, tumor-derived factors, inflammatory mediators and metabolic stress can redirect this repair program and generate tumor-associated Schwann cell (TASc) states. These cells are enriched around nerves and invasive fronts, where they have been linked to perineural invasion, extracellular matrix remodeling and changes in immune-cell localization and function. This Review summarizes recent work on the origin, recruitment and reprogramming of TAScs, with emphasis on molecular and cellular mechanisms rather than descriptive pathology alone. We discuss how transcriptional regulators, chemokines, neurotrophic factors, extracellular vesicles and metabolic pathways shape Schwann cell states in tumors, and how these states communicate with cancer cells, macrophages, T cells and natural killer cells. We also consider single-cell and spatial transcriptomic studies that have refined TASc classification, while noting the limitations of marker-based annotation, model systems and cross-tumor extrapolation. Finally, we evaluate the potential use of TASc-associated programs as biomarkers and therapeutic entry points, particularly in tumors with perineural invasion or resistance to immunotherapy. A critical assessment of current evidence indicates that Schwann cell reprogramming is a context-dependent process that should be studied as part of the wider cellular organization of the cancer microenvironment. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature B.V. FAU - Li, Hongbin AU - Li H AUID- ORCID: 0000-0003-2709-3633 AD - School of Life Science and Engineering, Lanzhou University of Technology, No.36 Pengjiaping Road, Lanzhou, 730050, Gansu Province, China. lihongbin@lut.edu.cn. AD - Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou, 730030, China. lihongbin@lut.edu.cn. FAU - Liu, Wenyang AU - Liu W AD - School of Life Science and Engineering, Lanzhou University of Technology, No.36 Pengjiaping Road, Lanzhou, 730050, Gansu Province, China. FAU - Liang, Jing AU - Liang J AD - School of Life Science and Engineering, Lanzhou University of Technology, No.36 Pengjiaping Road, Lanzhou, 730050, Gansu Province, China. FAU - Li, Yuan AU - Li Y AD - School of Life Science and Engineering, Lanzhou University of Technology, No.36 Pengjiaping Road, Lanzhou, 730050, Gansu Province, China. FAU - Pei, Jianying AU - Pei J AD - Department of Clinical Laboratory Center, Gansu Provincial Maternity and Child-Care Hospital (Gansu Provincial Central Hospital), Lanzhou, 730050, China. FAU - Zhang, Chong AU - Zhang C AD - Department of Clinical Laboratory Center, Gansu Provincial Maternity and Child-Care Hospital (Gansu Provincial Central Hospital), Lanzhou, 730050, China. zhch1972@163.com. LA - eng GR - 2026QNGR023/Provincial-level Talent Programs of Gansu Province/ GR - No. 82560536/National Natural Science Foundation of China/ GR - GSWSZD2025-17/Scientific Research Project of Health Industry in Gansu Province/ PT - Journal Article PT - Review DEP - 20261027 PL - Netherlands TA - Mol Biol Rep JT - Molecular biology reports JID - 0403234 SB - IM MH - *Schwann Cells/metabolism/immunology/pathology MH - Humans MH - *Tumor Microenvironment/immunology/genetics MH - Animals MH - *Cellular Reprogramming/immunology/genetics MH - *Neoplasms/immunology/pathology/metabolism OTO - NOTNLM OT - Immune regulation OT - Perineural invasion OT - Schwann cells OT - Spatial transcriptomics OT - Tumor microenvironment OT - Tumor-associated Schwann cells COIS- Declarations. Competing interests: The authors declare no competing interests. Ethical approval and consent to participate: Not applicable. Consent for publication: Not applicable. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:17 PHST- 2026/05/28 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:17 [entrez] AID - 10.1007/s11033-026-13000-5 [pii] AID - 10.1007/s11033-026-13000-5 [doi] PST - epublish SO - Mol Biol Rep. 2026 Oct 27;53(1):1837. doi: 10.1007/s11033-026-13000-5. PMID- 42944929 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1435-2451 (Electronic) IS - 1435-2443 (Print) IS - 1435-2443 (Linking) VI - 411 IP - 1 DP - 2026 Jul 30 TI - Comparison of laparoscopic and open gastrectomy in gastric cancer: a randomized clinical trial on sufficiency of lymph node dissection and perioperative outcomes. LID - 10.1007/s00423-026-04134-y [doi] LID - 296 AB - PURPOSE: To evaluate the oncological sufficiency and perioperative outcomes of laparoscopic versus open gastrectomy in patients with gastric adenocarcinoma, focusing on the sufficiency of lymph node dissection and short-term clinical results. METHODS: This single-center, single-blind, randomized clinical trial included 40 patients with gastric adenocarcinoma, allocated equally to undergo either open or laparoscopic gastrectomy with standard D2 lymphadenectomy. The primary outcome was the total number of dissected lymph nodes. Secondary outcomes included operative time, intraoperative blood loss, postoperative complications, and length of hospital stay. RESULTS: Both groups were comparable in demographic and baseline clinical characteristics. The mean number of dissected lymph nodes was similar between open (25.5 +/- 1.67) and laparoscopic (24.75 +/- 2.12) groups (p = 0.222), meeting oncologic adequacy criteria. Laparoscopic surgery resulted in significantly less blood loss (90.00 +/- 13.82 mL vs. 128.00 +/- 23.96 mL, p < 0.001) and shorter hospital stay (3.95 +/- 0.68 days vs. 6.65 +/- 0.93 days, p < 0.001), but required longer operative time (198.75 +/- 26.25 min vs. 114.00 +/- 18.93 min, p < 0.001). Postoperative complication rates were lower in the laparoscopic group (10.0% vs. 30.0%), though the difference was not statistically significant (p = 0.235). CONCLUSION: Laparoscopic gastrectomy is a safe and effective alternative to open surgery for gastric cancer, achieving comparable lymphadenectomy with advantages in blood loss and recovery time. These findings support the oncological safety and perioperative benefits of the minimally invasive approach when performed by experienced surgeons. CI - (c) 2026. The Author(s). FAU - Mohajeri, Babak AU - Mohajeri B AD - General Surgery Department, Shahid Modarres Hospital, Shahid Beheshti University of Medical Sciences, Saadat-Abad Avenue, Tehran, Iran. FAU - Malekpour Alamdari, Nasser AU - Malekpour Alamdari N AD - General Surgery Department, Shahid Modarres Hospital, Shahid Beheshti University of Medical Sciences, Saadat-Abad Avenue, Tehran, Iran. nassermalekpour@gmail.com. AD - Critical Care Quality Improvement Research Center, Shahid Modarres Hospital, Shahid Beheshti University of Medical Sciences, Saadat-Abad Avenue, Tehran, Iran. nassermalekpour@gmail.com. FAU - Abbasi, Maryam AU - Abbasi M AD - General Surgery Department, Shahid Modarres Hospital, Shahid Beheshti University of Medical Sciences, Saadat-Abad Avenue, Tehran, Iran. AD - Critical Care Quality Improvement Research Center, Shahid Modarres Hospital, Shahid Beheshti University of Medical Sciences, Saadat-Abad Avenue, Tehran, Iran. FAU - Esmaeili Rastaghi, Alireza AU - Esmaeili Rastaghi A AD - General Surgery Department, Shahid Modarres Hospital, Shahid Beheshti University of Medical Sciences, Saadat-Abad Avenue, Tehran, Iran. LA - eng PT - Comparative Study PT - Journal Article PT - Randomized Controlled Trial DEP - 20260730 PL - Germany TA - Langenbecks Arch Surg JT - Langenbeck's archives of surgery JID - 9808285 SB - IM MH - Humans MH - *Stomach Neoplasms/surgery/pathology/mortality MH - *Lymph Node Excision/methods MH - *Gastrectomy/methods MH - *Laparoscopy/methods MH - Female MH - Male MH - Middle Aged MH - *Adenocarcinoma/surgery/pathology/mortality MH - Single-Blind Method MH - Treatment Outcome MH - Length of Stay MH - Aged MH - Postoperative Complications/epidemiology MH - Operative Time MH - Blood Loss, Surgical PMC - PMC13714004 OTO - NOTNLM OT - D2 lymphadenectomy OT - Gastric cancer OT - Laparoscopic surgery OT - Open surgery OT - Perioperative outcomes COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/07/30 CRDT- 2026/10/27 12:17 PHST- 2025/08/03 00:00 [received] PHST- 2026/07/13 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:17 [entrez] PHST- 2026/07/30 00:00 [pmc-release] AID - 10.1007/s00423-026-04134-y [pii] AID - 4134 [pii] AID - 10.1007/s00423-026-04134-y [doi] PST - epublish SO - Langenbecks Arch Surg. 2026 Jul 30;411(1):296. doi: 10.1007/s00423-026-04134-y. PMID- 42944919 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1432-0843 (Electronic) IS - 0344-5704 (Print) IS - 0344-5704 (Linking) VI - 96 IP - 1 DP - 2026 Oct 27 TI - The role of tyrosine kinase inhibitors as a post-hematopoietic stem cell transplant continuation therapy for pediatric acute leukemia: a systematic review. LID - 10.1007/s00280-026-04976-9 [doi] LID - 126 AB - PURPOSE: Relapse following hematopoietic stem cell transplantation (HSCT) is a leading cause of transplant failure for pediatric acute leukemia patients, emphasizing the need for an effective post-transplant continuation therapy. This systematic review evaluates the role of tyrosine kinase inhibitors (TKIs) as a post-HSCT continuation therapy in pediatric acute leukemia. METHODS: A literature search of PubMed, Cochrane, Web of Science, and VHL was conducted according to PRISMA guidelines. Original studies were included if they were relevant to the research question and fit the eligibility criteria. RESULTS: The initial search identified 765 articles, with 16 studies included after filtration. TKIs were indicated for patients with chromosomal alterations causing constitutively active kinase signaling. Common agents included imatinib, dasatinib, ponatinib, sorafenib, and gilteritinib, and selection was based on disease status and molecular profiling. Duration of continuation ranged from 10 months to 3 years, with dosage dependent on the agent and tolerability. TKIs were generally well-tolerated, with reported side effects including cytopenia, liver dysfunction, and skin conditions. Across the included studies, MRD negativity and sustained molecular remission were reported in selected patients receiving post-HSCT TKI continuation therapy, while relapse was also reported after treatment discontinuation or in resistant disease. However, optimal duration and long-term safety remain unclear in the pediatric population. CONCLUSION: TKIs can serve as a targeted post-HSCT continuation therapy in select pediatric acute leukemia patients, particularly those with high-risk, mutation-driven disease. Molecular profiling and MRD monitoring are central to guiding agent selection, timing, and mutation-directed therapy adjustments. These findings support individualized TKI treatment to reduce relapse risk following HSCT, while highlighting the need for prospective studies to define standardized treatment strategies. CI - (c) 2026. The Author(s). FAU - Avineni, Mahati AU - Avineni M AD - College of Graduate Studies, Northeast Ohio Medical University, Rootstown, OH, 44272, USA. AD - College of Medicine, Northeast Ohio Medical University, 4209 St, OH-44, Rootstown, OH, 44272, USA. FAU - Habo, Yara K AU - Habo YK AD - College of Medicine, Northeast Ohio Medical University, 4209 St, OH-44, Rootstown, OH, 44272, USA. FAU - Habo, Nora K AU - Habo NK AD - College of Medicine, Northeast Ohio Medical University, 4209 St, OH-44, Rootstown, OH, 44272, USA. FAU - Elsayed, Ahmed Adham R AU - Elsayed AAR AD - College of Medicine, Northeast Ohio Medical University, 4209 St, OH-44, Rootstown, OH, 44272, USA. AD - Department of Surgery, Northeast Ohio Medical University, 4209 St, OH-44, Rootstown, OH, 44272, USA. AD - Department of Biomedical Sciences, Northeast Ohio Medical University, 4209 St, OH-44, Rootstown, OH, 44272, USA. FAU - Basson, Marc D AU - Basson MD AD - College of Medicine, Northeast Ohio Medical University, 4209 St, OH-44, Rootstown, OH, 44272, USA. mbasson@neomed.edu. AD - Department of Surgery, Northeast Ohio Medical University, 4209 St, OH-44, Rootstown, OH, 44272, USA. mbasson@neomed.edu. AD - Department of Biomedical Sciences, Northeast Ohio Medical University, 4209 St, OH-44, Rootstown, OH, 44272, USA. mbasson@neomed.edu. LA - eng PT - Journal Article PT - Review PT - Systematic Review DEP - 20261027 PL - Germany TA - Cancer Chemother Pharmacol JT - Cancer chemotherapy and pharmacology JID - 7806519 RN - 0 (Protein Kinase Inhibitors) RN - 0 (Tyrosine Kinase Inhibitors) RN - 0 (Antineoplastic Agents) SB - IM MH - Humans MH - *Protein Kinase Inhibitors/adverse effects/therapeutic use/administration & dosage MH - *Hematopoietic Stem Cell Transplantation/methods MH - Child MH - *Precursor Cell Lymphoblastic Leukemia-Lymphoma/therapy MH - *Leukemia/therapy MH - *Leukemia, Myeloid, Acute/therapy/drug therapy MH - Tyrosine Kinase Inhibitors MH - *Antineoplastic Agents/adverse effects/administration & dosage/therapeutic use PMC - PMC13714093 OTO - NOTNLM OT - Continuation therapy OT - Hematopoietic stem cell transplantation OT - Leukemia OT - Pediatric OT - Tyrosine kinase inhibitors COIS- Declarations. Conflict of interest: All authors declare they have no conflicts of interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 12:16 PHST- 2026/06/10 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:16 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 10.1007/s00280-026-04976-9 [pii] AID - 4976 [pii] AID - 10.1007/s00280-026-04976-9 [doi] PST - epublish SO - Cancer Chemother Pharmacol. 2026 Oct 27;96(1):126. doi: 10.1007/s00280-026-04976-9. PMID- 42944917 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1432-1904 (Electronic) IS - 0028-1042 (Print) IS - 0028-1042 (Linking) VI - 113 IP - 6 DP - 2026 Oct 27 TI - Rethinking hydrothermal vents: a structural ecology perspective on the early emergence of metabolic roles. LID - 10.1007/s00114-026-02189-x [doi] LID - 139 AB - Hydrothermal vents are widely considered plausible settings for the origin of life, owing to their steep redox gradients and the availability of chemical energy. Their capacity to support organic synthesis, molecular retention, and breakdown varies with local conditions. Here, I propose a conceptual framework for assessing whether connected environments could support complementary prebiotic chemical functions. Hydrothermal systems may contribute to the breakdown and reuse of supplied organic compounds alongside their constructive roles, while ice-water interfaces may support concentration, selected synthetic reactions, and product retention when suitable reactants are available. Producer-like, consumer-like, and decomposer-like functions are used as nonexclusive analogies for chemical production, use of supplied organic compounds, and breakdown yielding potentially reusable products; they do not denote demonstrated abiotic metabolisms. The central hypothesis is that life's beginnings may have depended partly on transitions between environments, rather than on processes within any single environment alone. This proposal can be assessed by comparing coupled and isolated reaction systems for product survival, reuse, and sustained reaction activity. The article presents no new experimental validation, and neither the proposed environmental connections nor their contribution to the emergence of heritable metabolism or the last universal common ancestor is established. CI - (c) 2026. The Author(s). FAU - Kato, Shinya AU - Kato S AUID- ORCID: 0000-0002-7199-3105 AD - Institute for Integrated Radiation and Nuclear Science, Kyoto University, 2 Asashiro-Nishi, Kumatori-cho, Sennan-gun, Osaka, 590-0494, Japan. kato.shinya.7w@kyoto-u.ac.jp. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Germany TA - Naturwissenschaften JT - Die Naturwissenschaften JID - 0400767 SB - IM MH - *Hydrothermal Vents/chemistry MH - *Origin of Life MH - Ecology PMC - PMC13714024 OTO - NOTNLM OT - Environmental structural ecology OT - Hydrothermal vents OT - Ice-water interfaces OT - Origin of life COIS- Declarations. Ethics, consent to participate, and consent to publish: Not applicable. Competing interests: The authors declare no competing interests. Clinical trial number: Not applicable. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 12:16 PHST- 2026/07/01 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:16 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 10.1007/s00114-026-02189-x [pii] AID - 2189 [pii] AID - 10.1007/s00114-026-02189-x [doi] PST - epublish SO - Naturwissenschaften. 2026 Oct 27;113(6):139. doi: 10.1007/s00114-026-02189-x. PMID- 42944915 OWN - NLM STAT- Publisher LR - 20261027 IS - 1432-1920 (Electronic) IS - 0028-3940 (Linking) DP - 2026 Oct 27 TI - An immediate reperfusion sign might increase the likelihood of favourable outcomes in patients with intracranial atherosclerotic disease-related stroke who received stent-retriever-based thrombectomy. LID - 10.1007/s00234-026-04226-y [doi] AB - OBJECTS: To investigate the relationship between the presence of an "immediate reperfusion sign (IRS)" during retrievable stent-based thrombectomy and clinical outcomes in patients with large vessel-occluded acute ischaemic stroke related to intracranial atherosclerotic disease (ICAD) treated with stent retrievers. METHODS: Consecutive ICAD-LVO stroke patients who were treated with stent retrievers and whose imaging records were available for the assessment of the IRS were included. The sensitivity, specificity, and predictive values of the IRS for predicting favourable outcomes were calculated. Univariate analysis and partial correlation analysis were also performed. RESULTS: Eighty-two ICAD-LVO patients were identified from 421 endovascularly treated AIS patients from 2015 to 2024. Fifty-four of them were ultimately included in the analysis, and an IRS was observed in 41 of these patients (75.9%). Favourable outcomes were significantly more frequent in the IRS (+) group (51.2% versus 7.7%, p=0.005). The sensitivity of the IRS for predicting favourable outcomes was 95.5% (21/22), the specificity was 37.5% (12/32), the positive predictive value was 51.2% (21/41), and the negative predictive value (NPV) was 92.3% (12/13). Additionally, the presence of an IRS, as well as age, baseline NIHSS score and successful final reperfusion, was significantly different between the favourable and unfavourable groups. Partial correlation analysis revealed a moderate correlation (r=0.412; p=0.002) between the comprehensive reperfusion score (in which the presence of an IRS and successful final reperfusion was counted as 1 point) and favourable outcomes. CONCLUSION: In our respective cohort of patients, the presence of an IRS during stent-retriever-based thrombectomy appeared to indicate a higher rate of first-pass-effect and a 3-month favourable outcome in acute LVO stroke patients with ICAD. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Wen, Wanling AU - Wen W AD - Devision 5, Research Department, The Ninth Medical Center of Military General Hospital, PLA, Beijing, China. FAU - Cui, Yongqiang AU - Cui Y AD - Neurology, The Ninth Medical Center of Military General Hospital, PLA, Beijing, China. FAU - Lin, Tian AU - Lin T AD - Neurology, The Ninth Medical Center of Military General Hospital, PLA, Beijing, China. FAU - Kong, Xiangkai AU - Kong X AD - Neurology, The Ninth Medical Center of Military General Hospital, PLA, Beijing, China. FAU - Du, Bin AU - Du B AD - Neurology, The Ninth Medical Center of Military General Hospital, PLA, Beijing, China. FAU - Duan, Wenbo AU - Duan W AD - Neurology, The Ninth Medical Center of Military General Hospital, PLA, Beijing, China. FAU - Zhang, Xiaoyun AU - Zhang X AD - Devision 5, Research Department, The Ninth Medical Center of Military General Hospital, PLA, Beijing, China. FAU - Han, Xue AU - Han X AD - Neurology, The Ninth Medical Center of Military General Hospital, PLA, Beijing, China. FAU - Wang, Weiying AU - Wang W AD - Neurology, The Ninth Medical Center of Military General Hospital, PLA, Beijing, China. FAU - Xuan, Xuan AU - Xuan X AD - Neurology, The Ninth Medical Center of Military General Hospital, PLA, Beijing, China. FAU - Lu, Dongxu AU - Lu D AD - Neurology, The Ninth Medical Center of Military General Hospital, PLA, Beijing, China. FAU - Yu, Yijiao AU - Yu Y AD - Neurology, The Ninth Medical Center of Military General Hospital, PLA, Beijing, China. FAU - Liu, Yanli AU - Liu Y AD - Neurology, The Ninth Medical Center of Military General Hospital, PLA, Beijing, China. FAU - Cai, Yiling AU - Cai Y AD - Neurology, The Ninth Medical Center of Military General Hospital, PLA, Beijing, China. FAU - Liu, Li AU - Liu L AD - Neurology, The Ninth Medical Center of Military General Hospital, PLA, Beijing, China. snkjyb40207@sina.cn. FAU - Du, Juan AU - Du J AD - Neurology, The Ninth Medical Center of Military General Hospital, PLA, Beijing, China. juanzijuanzi@263.net. LA - eng PT - Journal Article DEP - 20261027 PL - Germany TA - Neuroradiology JT - Neuroradiology JID - 1302751 SB - IM OTO - NOTNLM OT - Digital subtraction angiography OT - Intracranial atherosclerosis OT - Ischaemic stroke OT - Stent COIS- Declaration. Competing interests: The authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:16 PHST- 2026/07/16 00:00 [received] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:16 [entrez] AID - 10.1007/s00234-026-04226-y [pii] AID - 10.1007/s00234-026-04226-y [doi] PST - aheadofprint SO - Neuroradiology. 2026 Oct 27. doi: 10.1007/s00234-026-04226-y. PMID- 42944911 OWN - NLM STAT- Publisher LR - 20261027 IS - 2366-0058 (Electronic) DP - 2026 Oct 27 TI - Imaging classification of pancreatic cystic lesions: a radiologist's guide. LID - 10.1007/s00261-026-05847-w [doi] AB - Pancreatic cystic lesions (PCLs) are increasingly encountered in clinical practice owing to the widespread use of high-resolution cross-sectional imaging. These lesions comprise a heterogeneous group of entities ranging from benign cysts to pre-malignant neoplasms and invasive cancers, making accurate characterization essential for appropriate patient surveillance and therapeutic management. Imaging plays a pivotal role in lesion detection, differential diagnosis, and assessment of malignant potential. This review presents a practical imaging-based classification of PCLs founded on lesion morphology, relationship to the pancreatic ductal system, enhancement patterns, and associated imaging features. Using a stepwise diagnostic approach, pancreatic cystic lesions are categorized according to their increasing risk of malignancy into non-neoplastic cysts, serous cystic neoplasms, mucinous cystic lesions, and cystic degeneration of solid pancreatic tumors. Characteristic imaging findings, relevant differential diagnostic considerations, and imaging features associated with increased malignant potential are comprehensively reviewed, with particular emphasis on the role of MRI and MR cholangiopancreatography (MRCP) in lesion characterization. A structured pattern-recognition flowchart is proposed to facilitate routine image interpretation and enhance diagnostic confidence. In addition, a practical reporting template is presented to promote standardized assessment and effective communication within multidisciplinary teams. Emerging techniques, including diffusion-weighted imaging, quantitative MRI biomarkers, radiomics, artificial intelligence, and molecular analysis of cyst fluid, are discussed as promising tools for improving diagnostic accuracy and refining risk stratification. By emphasizing readily recognizable imaging features and a systematic diagnostic workflow, this review provides radiologists with a practical and clinically applicable framework for the evaluation, diagnosis, risk assessment, and multidisciplinary management of pancreatic cystic lesions. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Boraschi, Piero AU - Boraschi P AUID- ORCID: 0000-0001-5655-3921 AD - Department of Diagnostic Imaging, Azienda Tutela della Salute (ATS) Liguria - Area Sociosanitaria Locale 5, La Spezia, Italy. p.boraschi@gmail.com. FAU - Cervelli, Rosa AU - Cervelli R AUID- ORCID: 0000-0002-4570-8737 AD - Department of Radiological Nuclear and Laboratory Medicine, Azienda Ospedaliera Universitaria Pisana, Pisa, Italy. FAU - Donati, Francescamaria AU - Donati F AUID- ORCID: 0000-0002-0608-8360 AD - Department of Radiological Nuclear and Laboratory Medicine, Azienda Ospedaliera Universitaria Pisana, Pisa, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - United States TA - Abdom Radiol (NY) JT - Abdominal radiology (New York) JID - 101674571 SB - IM OTO - NOTNLM OT - Differential diagnosis OT - MRCP OT - MRI OT - Pancreatic cystic lesions OT - Pancreatic cystic neoplasms OT - Risk stratification OT - Structured reporting COIS- Declarations. Competing interests: The authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:16 PHST- 2026/07/04 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:16 [entrez] AID - 10.1007/s00261-026-05847-w [pii] AID - 10.1007/s00261-026-05847-w [doi] PST - aheadofprint SO - Abdom Radiol (NY). 2026 Oct 27. doi: 10.1007/s00261-026-05847-w. PMID- 42944910 OWN - NLM STAT- Publisher LR - 20261027 IS - 2366-0058 (Electronic) DP - 2026 Oct 27 TI - Preoperative risk stratification for anastomotic leakage after gastrectomy: a model based on dual‑energy CT extracellular volume fraction and sarcopenia. LID - 10.1007/s00261-026-05839-w [doi] AB - PURPOSE: To develop a purely preoperative risk stratification model for anastomotic leakage (AL) after radical gastrectomy using clinical, dual-energy CT (DECT), and body composition variables available before surgery. METHODS: This retrospective study included 439 patients with gastric cancer who underwent radical gastrectomy. Preoperative clinical, DECT, and body composition data were collected. A domain-based predictor selection strategy was used, followed by Firth penalized logistic regression to account for the low event rate. Internal validation was performed using 1000 bootstrap resamples, with an additional full-pipeline bootstrap sensitivity analysis. Model performance was assessed by discrimination, calibration, decision curve analysis (DCA), and risk stratification. RESULTS: AL occurred in 27 patients (6.15%). The final model included cardia tumor location (OR = 4.44), higher ECV (OR = 1.09 per 1%), and sarcopenia (OR = 3.66). The model showed good discrimination, with an apparent AUC of 0.853 and a bootstrap-corrected AUC of 0.843. Full-pipeline bootstrap sensitivity analysis yielded an optimism-corrected AUC of 0.833. Calibration was acceptable, with a Brier score of 0.050, and DCA suggested potential net benefit. Observed AL rates increased stepwise across low-risk (< 3%), intermediate-risk (3%-10%), and high-risk (>/= 10%) groups: 1.23%, 5.13%, and 22.78%, respectively. The high-risk group comprised 18.0% of the cohort but accounted for 66.7% of all AL events. CONCLUSION: A concise model integrating tumor location, DECT-derived ECV, and sarcopenia showed favorable performance for preoperative AL risk stratification after gastrectomy. This internally validated model may help preoperative AL risk stratification, but multicenter external validation and impact analysis are required before implementation. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Ren, Tiezhu AU - Ren T AD - Department of Radiology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China. FAU - You, Haibo AU - You H AD - Department of Radiology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China. FAU - Liu, Xinyu AU - Liu X AD - Department of Radiology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China. FAU - Ren, JiaLiang AU - Ren J AD - Department of Information Center, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. FAU - Ma, Long AU - Ma L AD - Department of Radiology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China. FAU - Zhang, Chenyang AU - Zhang C AD - Department of Radiology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China. FAU - Bao, Jianlin AU - Bao J AD - Department of Radiology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China. FAU - Xu, Min AU - Xu M AD - Department of Radiology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China. FAU - Peng, Yue AU - Peng Y AUID- ORCID: 0009-0003-6242-1388 AD - Department of Radiology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China. FAU - Xu, Yuan AU - Xu Y AD - Department of Radiology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China. FAU - Sun, Jiachen AU - Sun J AD - Department of Radiology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China. FAU - Su, Xinan AU - Su X AD - Department of Rheumatology, The First Hospital of Lanzhou University, Lanzhou, China. FAU - Zhang, Wenjuan AU - Zhang W AD - Department of Radiology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China. hxzhangwj121@163.com. FAU - Zhou, Junlin AU - Zhou J AUID- ORCID: 0000-0001-8109-6347 AD - Department of Radiology, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China. ery_zhoujl@lzu.edu.cn. LA - eng GR - 26YFFA011/Provincial Science and Technology Program (Key Research and DevelopmentProgram)/ GR - 2023-2-44/Lanzhou Youth Science and Technology Talent Innovation Project/ GR - 2025-21-zdfy-003/Key Incubation Project Funds of the second hospital & clinical medical school.lanzhou university/ GR - 24JRRA921/Major Project of Joint Scientific Research Fund of Gansu Province/ PT - Journal Article DEP - 20261027 PL - United States TA - Abdom Radiol (NY) JT - Abdominal radiology (New York) JID - 101674571 SB - IM OTO - NOTNLM OT - Anastomotic leakage OT - Dual-energy CT OT - Gastric cancer OT - Sarcopenia COIS- Declarations. Conflict of interest: The authors declare no competing interests. Ethical approval: This retrospective study was reviewed and approved by the Ethics Committee of Lanzhou University Second Hospital (Approval No. 2024 A-1401). The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013) and all relevant institutional guidelines and regulations. The need for written informed consent was waived by the ethics committee due to the retrospective nature of the study. Consent to participate: This retrospective study was reviewed and approved by the Ethics Committee of Lanzhou University Second Hospital (Approval No. 2024 A-1401). The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013) and all relevant institutional guidelines and regulations. The need for written informed consent was waived by the ethics committee due to the retrospective nature of the study. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:16 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:16 [entrez] AID - 10.1007/s00261-026-05839-w [pii] AID - 10.1007/s00261-026-05839-w [doi] PST - aheadofprint SO - Abdom Radiol (NY). 2026 Oct 27. doi: 10.1007/s00261-026-05839-w. PMID- 42944905 OWN - NLM STAT- Publisher LR - 20261027 IS - 2731-7072 (Electronic) IS - 2731-7064 (Linking) DP - 2026 Oct 27 TI - [Female-to-male reassignment surgeries: metaidoioplasty and phalloplasty]. LID - 10.1007/s00120-026-02941-1 [doi] AB - Metoidioplasty is an established gender-affirming genital surgical procedure and represents a less invasive alternative to phalloplasty using pedicled or free tissue flaps. Mobilization and lengthening of the testosterone-enlarged clitoris, combined with construction of an elongated neourethra, results in both esthetic and functional masculinization of the external genitalia. In many patients micturition in a standing position can be achieved. Outcomes largely depend on the individual anatomical conditions. The main advantages of metoidioplasty include preservation of erogenous sensation and orgasmic function, low postoperative morbidity, and avoidance of extensive donor-site scars. However, penile length and rigidity sufficient for penetrative sexual intercourse are generally not achieved. The reconstructive goal of phalloplasty for gender affirmation is to create an esthetically pleasing phallus that allows for standing urination and, if desired, penetration during sexual intercourse. Depending on the technique, pedicled or free flap procedures are used. The most commonly used technique is the free radial forearm flap, which has now become established as the gold standard in many centers. CI - (c) 2026. The Author(s), under exclusive licence to Springer Medizin Verlag GmbH, ein Teil von Springer Nature. FAU - Szyper, Lisa Maria AU - Szyper LM AD - TransgenderZentrum, Dr. Lubos Kliniken Munchen, Denninger Str. 44, 81679, Munchen, Deutschland. FAU - Wagner, Johannes Maximilian AU - Wagner JM AD - Zentrum fur Transgenderchirurgie, Klinik fur Plastische, Rekonstruktive und Asthetische Chirurgie, KEM Evang. Kliniken Essen-Mitte gGmbH, Henricistr. 92, 45136, Essen, Deutschland. j.wagner@kem-med.com. FAU - Behr, Bjorn AU - Behr B AD - Zentrum fur Transgenderchirurgie, Klinik fur Plastische, Rekonstruktive und Asthetische Chirurgie, KEM Evang. Kliniken Essen-Mitte gGmbH, Henricistr. 92, 45136, Essen, Deutschland. FAU - Bohr, Julia AU - Bohr J AD - Zentrum fur Transgenderchirurgie, Klinik fur Plastische, Rekonstruktive und Asthetische Chirurgie, KEM Evang. Kliniken Essen-Mitte gGmbH, Henricistr. 92, 45136, Essen, Deutschland. FAU - Markovsky, Oliver AU - Markovsky O AD - TransgenderZentrum, Dr. Lubos Kliniken Munchen, Denninger Str. 44, 81679, Munchen, Deutschland. urologie@lubos-kliniken.de. LA - ger PT - English Abstract PT - Journal Article PT - Review TT - Maskulinisierende Operationen: Metaidoio- und Phalloplastik. DEP - 20261027 PL - Germany TA - Urologie JT - Urologie (Heidelberg, Germany) JID - 9918384886606676 SB - IM OTO - NOTNLM OT - Gender affirmation surgery OT - Gender dysphoria OT - Micturition OT - Urethral strictures OT - Urethrocutaneous fistulas COIS- Einhaltung ethischer Richtlinien. Interessenkonflikt: L.M. Szyper, J.M. Wagner, B. Behr, J. Bohr und O. Markovsky geben an, dass kein Interessenkonflikt besteht. Fur diesen Beitrag wurden von den Autor/-innen keine Studien an Menschen oder Tieren durchgefuhrt. Fur die aufgefuhrten Studien gelten die jeweils dort angegebenen ethischen Richtlinien. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:15 PHST- 2026/09/10 00:00 [received] PHST- 2026/09/10 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:15 [entrez] AID - 10.1007/s00120-026-02941-1 [pii] AID - 10.1007/s00120-026-02941-1 [doi] PST - aheadofprint SO - Urologie. 2026 Oct 27. doi: 10.1007/s00120-026-02941-1. PMID- 42944901 OWN - NLM STAT- Publisher LR - 20261027 IS - 1435-1544 (Electronic) IS - 0938-7412 (Linking) DP - 2026 Oct 27 TI - [Three-dimensional electroanatomic mapping in the electrophysiology lab: when, how and why? : A practical introduction for early-career electrophysiologists]. LID - 10.1007/s00399-026-01179-w [doi] AB - Three-dimensional electroanatomic mapping has become an integral part of modern invasive electrophysiology. It combines catheter localization, patient-specific cardiac anatomy, local electrograms, activation timing, voltage information, and ablation lesion documentation within a spatial working model. For early-career electrophysiologists, however, the interpretation of these maps can be challenging. This review provides a practical introduction to three-dimensional (3D) mapping for young electrophysiologists during their first steps in the electrophysiology laboratory. It addresses when 3D mapping is useful or necessary, how a map is created, what information can be derived from different map types, and which pitfalls should be avoided. The article summarizes technical principles and clinical applications of 3D electroanatomic mapping based on current expert consensus documents, review articles, and practical electrophysiological concepts. 3D mapping is particularly valuable in atrial fibrillation ablation, atypical atrial tachycardias, ventricular tachycardias, redo procedures, congenital or postsurgical anatomy, and low-fluoroscopy workflows. Activation maps help visualize the temporal sequence of electrical propagation, whereas voltage maps provide information on scar, low-voltage areas, and potential arrhythmogenic substrate. The quality of a map depends on stable rhythm, reliable temporal and spatial references, catheter contact, signal quality, adequate point density, and correct annotation. 3D mapping is a powerful tool for navigation, mechanism analysis, substrate characterization, and ablation documentation. Nevertheless, it does not replace electrophysiological reasoning. Every map must be interpreted critically and validated using intracardiac electrograms, surface ECG, anatomy, pacing maneuvers, and defined procedural endpoints. CI - (c) 2026. The Author(s), under exclusive licence to Springer Medizin Verlag GmbH, ein Teil von Springer Nature. FAU - Aweimer, Assem AU - Aweimer A AD - Klinik fur Kardiologie und Angiologie, Bereich Elektrophysiologie, BG Universitatsklinikum Bergmannsheil der Ruhr Universitat Bochum, Burkle-de-la-Camp-Platz 1, 44789, Bochum, Deutschland. assem.aweimer@rub.de. FAU - Beck, Patrick AU - Beck P AD - Klinik fur Kardiologie und Angiologie, Bereich Elektrophysiologie, BG Universitatsklinikum Bergmannsheil der Ruhr Universitat Bochum, Burkle-de-la-Camp-Platz 1, 44789, Bochum, Deutschland. LA - ger PT - English Abstract PT - Journal Article TT - 3D-Mapping im EP-Labor: Wann, wie und warum? : Ein praktischer Einstieg fur junge Elektrophysiologinnen und Elektrophysiologen. DEP - 20261027 PL - Germany TA - Herzschrittmacherther Elektrophysiol JT - Herzschrittmachertherapie & Elektrophysiologie JID - 9425873 SB - IM OTO - NOTNLM OT - Activation maps OT - Catheter ablation OT - Electroanatomic mapping OT - Electrophysiology OT - Voltage maps COIS- Einhaltung ethischer Richtlinien. Interessenkonflikt: A. Aweimer und P. Beck geben an, dass kein Interessenkonflikt besteht. Fur diesen Beitrag wurden von den Autoren keine Studien an Menschen oder Tieren durchgefuhrt. Die verwendeten klinischen Abbildungen wurden anonymisiert; ein Ruckschluss auf einzelne Patientinnen oder Patienten ist nicht moglich. Fur die zitierten Studien gelten die jeweils dort angegebenen ethischen Richtlinien. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:15 PHST- 2026/07/16 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:15 [entrez] AID - 10.1007/s00399-026-01179-w [pii] AID - 10.1007/s00399-026-01179-w [doi] PST - aheadofprint SO - Herzschrittmacherther Elektrophysiol. 2026 Oct 27. doi: 10.1007/s00399-026-01179-w. PMID- 42944897 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1432-1939 (Electronic) IS - 0029-8549 (Print) IS - 0029-8549 (Linking) VI - 208 IP - 11 DP - 2026 Oct 27 TI - Selective mortality leads to competitive release of a foundational perennial grass in an aridifying grassland. LID - 10.1007/s00442-026-05935-0 [doi] LID - 160 AB - Vegetation dynamics in dryland ecosystems are defined by competition for water, a limiting and increasingly variable resource. As aridification creates warmer and drier conditions, with more frequent and intense droughts, the nature of competitive relationships may be altered, leading to changes in community composition. Reduced water availability is expected to decrease the cover and abundance of cool-season C(3) grasses on the Colorado Plateau, with unknown impacts on other plant functional types, such as drought-tolerant C(4) species. Here, we explore the effects of competitive release on Pleuraphis jamesii, a dominant C(4) grass with rooting and photosynthetic traits that may enable it to capitalize on the mortality of less drought tolerant C(3) species. To accomplish this, we used herbicide to create three removal treatments (C(3) grass and forb removal, C(4) grass removal, and control (no removal)) surrounding P. jamesii focal individuals and measured the subsequent impacts on water availability and plant water relations and growth. The experimentally induced mortality resulted in greater water availability for focal individuals in the C(3) removal treatment. Increased water availability in C(3) removal plots translated to less water stress and greater biomass production of focal P. jamesii plants, suggesting that competitive release can serve as the basis for shifts in community composition towards drought tolerant C(4) plants observed regionally. These responses were not observed in the C(4) removal treatment despite removing roughly equivalent amounts of total plant cover. Our results suggest that reductions in C(3) competitors may benefit drought-tolerant C(4) species through increased resource availability. These findings contribute to a mechanistic understanding of competitive interactions in increasingly water-limited systems. CI - (c) 2026. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply. FAU - Hoy-Skubik, Sean L AU - Hoy-Skubik SL AUID- ORCID: 0000-0002-9157-0126 AD - Department of Ecology, Montana State University, Bozeman, MT, US. seanhoyskubik@montana.edu. AD - Southwest Biological Science Center, U.S. Geological Survey, Moab, UT, USA. seanhoyskubik@montana.edu. FAU - Hoover, David L AU - Hoover DL AD - Crops Research Laboratory, USDA-ARS Rangeland Resources and Systems Research Unity, Fort Collins, CO, USA. FAU - Pfennigwerth, Alix A AU - Pfennigwerth AA AD - Southwest Biological Science Center, U.S. Geological Survey, Moab, UT, USA. FAU - Knight, Anna C AU - Knight AC AD - Southwest Biological Science Center, U.S. Geological Survey, Moab, UT, USA. FAU - Duniway, Michael C AU - Duniway MC AD - Southwest Biological Science Center, U.S. Geological Survey, Moab, UT, USA. LA - eng PT - Journal Article DEP - 20261027 PL - Germany TA - Oecologia JT - Oecologia JID - 0150372 RN - 059QF0KO0R (Water) SB - IM MH - *Grassland MH - *Poaceae MH - Droughts MH - Drought Resistance MH - Ecosystem MH - Water MH - Biomass PMC - PMC13714021 OTO - NOTNLM OT - Community shifts OT - Competitive release OT - Drought OT - Drylands OT - Physiology COIS- Declarations. Conflicts of interest: The authors declare that they have no conflict of interest. Consent to participate: Not applicable. Consent for publication: Not applicable. Ethics approval: Not applicable. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 12:15 PHST- 2026/02/03 00:00 [received] PHST- 2026/07/02 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:15 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 10.1007/s00442-026-05935-0 [pii] AID - 5935 [pii] AID - 10.1007/s00442-026-05935-0 [doi] PST - epublish SO - Oecologia. 2026 Oct 27;208(11):160. doi: 10.1007/s00442-026-05935-0. PMID- 42944895 OWN - NLM STAT- Publisher LR - 20261027 IS - 1433-3023 (Electronic) IS - 0937-3462 (Linking) DP - 2026 Oct 27 TI - Brisk Walking- and Running-Induced Lower Urinary Tract Symptoms Questionnaire in Runners: French Translation and Content Validity. LID - 10.1007/s00192-026-06918-8 [doi] AB - INTRODUCTION AND HYPOTHESIS: Urinary incontinence (UI) during exercise is common among active females, with one in three runners experiencing symptoms. As UI can limit participation in sport, there is a need to understand exercise-specific symptoms and strategies used to mitigate them. Existing questionnaires do not assess UI that occurs specifically during exercise. The Brisk Walking- and Running-induced Lower Urinary Tract Symptoms Questionnaire (BRUTS-Q) was developed in English using focus groups and a Delphi process and is currently under validation. A French version is needed for francophone populations. The objectives were to translate the BRUTS-Q into French and to assess the content validity of the translated version among females reporting running-related UI in terms of relevance, comprehensibility, and comprehensiveness. METHODS: Following COnsensus-based Standards for the selection of health Measurement INstruments guidelines, a qualitative design using cognitive "think-aloud" interviews was conducted with French-speaking females who reported experiencing running-related UI. Iterative analyses were performed in sets of five interviews. Two researchers independently coded data to identify issues related to content validity, and the questionnaire was revised accordingly between rounds. The process continued until no new issues emerged. RESULTS: The final French version of the BRUTS-Q demonstrated strong content validity, with participants reporting good clarity, relevance, and comprehensiveness for describing running-related UI in the francophone female population. CONCLUSION: These findings support the strong potential of the French-language BRUTS-Q as a patient-centered outcome measure to assess symptoms of running-related UI and to inform the development of targeted interventions. Further studies are needed to evaluate its psychometric properties before it is used clinically or in research. CI - (c) 2026. The Author(s), under exclusive license to International Urogynecological Association. FAU - Proulx, Laurie AU - Proulx L AUID- ORCID: 0009-0008-1284-3386 AD - School of Rehabilitation Sciences, Faculty of Medicine, Universite Laval, 1050 avenue de la Medecine, Quebec, G1V 0A6, Canada. AD - Interdisciplinary Research Center on Rehabilitation and Social Integration (Cirris), Sante Quebec Capitale-Nationale - Universitaire, Quebec, Canada. FAU - McLean, Linda AU - McLean L AD - Motor Function Measurement Lab, School of Rehabilitation Sciences, Faculty of Health Sciences, University of Ottawa, Ottawa, Ontario, Canada. FAU - Klahsen, Olena AU - Klahsen O AD - Motor Function Measurement Lab, School of Rehabilitation Sciences, Faculty of Health Sciences, University of Ottawa, Ottawa, Ontario, Canada. FAU - Stock, Peyton AU - Stock P AD - Motor Function Measurement Lab, School of Rehabilitation Sciences, Faculty of Health Sciences, University of Ottawa, Ottawa, Ontario, Canada. FAU - Bernard, Stephanie AU - Bernard S AUID- ORCID: 0000-0003-1454-8555 AD - School of Rehabilitation Sciences, Faculty of Medicine, Universite Laval, 1050 avenue de la Medecine, Quebec, G1V 0A6, Canada. stephanie.bernard@fmed.ulaval.ca. AD - Interdisciplinary Research Center on Rehabilitation and Social Integration (Cirris), Sante Quebec Capitale-Nationale - Universitaire, Quebec, Canada. stephanie.bernard@fmed.ulaval.ca. LA - eng GR - 18000/Consortium national de formation en sante, Volet Universite d'Ottawa/ GR - 18000/Scholarship from: Centre for Interdisciplinary Research in Rehabilitation and Social Integration/ PT - Journal Article DEP - 20261027 PL - England TA - Int Urogynecol J JT - International urogynecology journal JID - 101567041 SB - IM OTO - NOTNLM OT - Content validity OT - Exercise-induced urinary incontinence OT - French translation OT - Pelvic health OT - Running COIS- Declarations. Ethical Considerations: This project was approved by the Research Ethics Committee of the CIUSSS de la Capitale-Nationale (approval number 2025-3137/02-07-2024). Conflicts of Interest: L.M. holds research grants from the Canadian Institutes of Health Research and the Natural Sciences and Engineering Research Council of Canada. She is scientific advisor to CNTRL+ Canada (unpaid) and Freyya USA (paid). S B. holds research grants from the Canadian Cancer Society and the Fonds de recherche du Quebec. None of the other authors has any conflicts of interest to declare. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:15 PHST- 2026/05/16 00:00 [received] PHST- 2026/09/11 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:15 [entrez] AID - 10.1007/s00192-026-06918-8 [pii] AID - 10.1007/s00192-026-06918-8 [doi] PST - aheadofprint SO - Int Urogynecol J. 2026 Oct 27. doi: 10.1007/s00192-026-06918-8. PMID- 42944893 OWN - NLM STAT- Publisher LR - 20261027 IS - 1618-2650 (Electronic) IS - 1618-2642 (Linking) DP - 2026 Oct 27 TI - Spatial metabolic insights of Pseudomonas aeruginosa biofilm treatments using stable isotope labeling with DESI mass spectrometry imaging. LID - 10.1007/s00216-026-06883-7 [doi] AB - Bacteria form biofilms on diverse substrates, and these communities can cause persistent infections owing to their elevated antibiotic resistance. Pseudomonas aeruginosa is a major cause of chronic infections because of its ability to form biofilms, which makes it difficult to eradicate with conventional antibiotics. The metabolic response of P. aeruginosa biofilms to antibiotic treatment, however, remains only partially understood. Here, desorption electrospray ionization mass spectrometry imaging (DESI-MSI) was used to map the spatial metabolic responses of mature and developing P. aeruginosa biofilms to ciprofloxacin and two ciprofloxacin-nitroxide hybrids. Stable isotope labeling of metabolites was used to support the spatial interpretation of quorum-sensing (QS) metabolites within the chemically complex and dynamic spatial environment of biofilms and to understand their biosynthetic pathway. Ciprofloxacin induced localized metabolic changes and selective reorganization of quorum-sensing and redox-associated metabolites in biofilms. Both hybrids underwent heterogeneous in situ reduction to their inactive hydroxylamine forms within biofilms. These results indicate that DESI-MSI with stable isotope labeling can resolve growth-stage-dependent colony biofilm metabolism and characterize the metabolic response of biofilms to antibiotic treatment. CI - (c) 2026. The Author(s). FAU - Qiu, Min AU - Qiu M AD - Department of Biosciences, School of Natural Sciences, Technical University of Munich (TUM), Lichtenbergstrasse 4, 85748, Munich, Germany. FAU - Wiedbrauk, Sandra AU - Wiedbrauk S AD - School of Chemistry and Physics, Faculty of Science and Center for Materials Science, Queensland University of Technology, 2 George Street, Brisbane, 4000, Australia. FAU - Fairfull-Smith, Kathryn E AU - Fairfull-Smith KE AD - School of Chemistry and Physics, Faculty of Science and Center for Materials Science, Queensland University of Technology, 2 George Street, Brisbane, 4000, Australia. FAU - Strittmatter, Nicole AU - Strittmatter N AUID- ORCID: 0000-0003-1277-9608 AD - Department of Biosciences, School of Natural Sciences, Technical University of Munich (TUM), Lichtenbergstrasse 4, 85748, Munich, Germany. nicole.strittmatter@tum.de. LA - eng GR - 202208320033/Chinese Scholarship Council/ GR - H-1258-24/Bavarian Ministry of Science and the Arts and the Queensland Government/ GR - C24-0918830768/RSC Researcher Collaborations Grant/ PT - Journal Article DEP - 20261027 PL - Germany TA - Anal Bioanal Chem JT - Analytical and bioanalytical chemistry JID - 101134327 SB - IM OTO - NOTNLM OT - Pseudomonas aeruginosa biofilms OT - Ciprofloxacin OT - Mass spectrometry imaging OT - Nitroxide hybrids OT - Stable isotope labeling COIS- Declarations. Conflict of interest: The authors have no conflicts of interest to disclose. Nicole Strittmatter is member of ABC's International Advisory Board but was not involved in the peer review of this paper. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:15 PHST- 2026/08/09 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:15 [entrez] AID - 10.1007/s00216-026-06883-7 [pii] AID - 10.1007/s00216-026-06883-7 [doi] PST - aheadofprint SO - Anal Bioanal Chem. 2026 Oct 27. doi: 10.1007/s00216-026-06883-7. PMID- 42944891 OWN - NLM STAT- Publisher LR - 20261027 IS - 1618-2650 (Electronic) IS - 1618-2642 (Linking) DP - 2026 Oct 27 TI - Indirect detection of trichlorfon in agricultural products based on Raman silent region. LID - 10.1007/s00216-026-06885-5 [doi] AB - In this study, the copper-based prussian blue analogue coated gold nanoparticles (Au@Cu-PBA NPs) were prepared by simple in situ etching method and firstly applied as surface-enhanced Raman scattering (SERS) tags for indirectly analyzing trichlorfon. The obtained Au@Cu-PBA NPs presented core-shell structure and exhibited an only sharp signal peak at 2175 cm(-1) in Raman silent region. The indirect SERS method was constructed for detecting trichlorfon in agricultural products based on competitive binding between trichlorfon and capture probes (Fe(3)O(4)@cApt) for signal probes (Au@Cu-PBA NPs@Apt). Under optimized competitive conditions (200 nM Apt, 300 nM cApt, 0.075 mg mL(-1) Fe(3)O(4)-COOH, 25 min at 35 degrees C for both incubation and competitive binding), the indirect SERS method showed good linear relationship between relative Raman intensity and logarithm of trichlorfon concentration in the range of 100-10,000 ng mL(-1). The limits of detection were from 16.6 to 22.3 ng g(-1) in cabbage, cucumber, apple and pear. The satisfactory recoveries (82.0%-90.3%) with relative standard deviations of 1.33%-5.95% were achieved. Benefiting from the synergy of specific recognition of trichlorfon by aptamer in binding process and spectral anti-interference of PBA tags in Raman silent region, this proposed method was finally proved to be sensitive, accurate and practical, especially possessing distinct advantages of high selectivity and excellent anti-interference capability for detecting trace trichlorfon in complex agricultural products. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature. FAU - Zhang, Chenye AU - Zhang C AD - College of Life Science, Yantai University, Yantai, 264005, P. R. China. FAU - Zhang, Xinyu AU - Zhang X AD - College of Life Science, Yantai University, Yantai, 264005, P. R. China. FAU - Cong, Shuang AU - Cong S AD - College of Life Science, Yantai University, Yantai, 264005, P. R. China. FAU - Zhang, Ziping AU - Zhang Z AD - College of Life Science, Yantai University, Yantai, 264005, P. R. China. FAU - Cao, Xiaolin AU - Cao X AD - College of Life Science, Yantai University, Yantai, 264005, P. R. China. aicaoxiaolin@126.com. LA - eng GR - 32302216/National Natural Science Foundation of China/ GR - 2023KJ241/the Program for Scientific Research Innovation Team of Young Scholar in Colleges and Universities of Shandong Province/ GR - YDZX2024017/the Technology Innovation Guidance Plan of Shandong Province/ GR - SDAST2025QTB013/Young Talent of Lifting engineering for Science and Technology in Shandong/ PT - Journal Article DEP - 20261027 PL - Germany TA - Anal Bioanal Chem JT - Analytical and bioanalytical chemistry JID - 101134327 SB - IM OTO - NOTNLM OT - Indirect detection OT - Prussian blue analogues OT - Raman silent region OT - Surface-enhanced Raman spectroscopy OT - Trichlorfon COIS- Declarations. Conflict of interest: The authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:15 PHST- 2026/08/01 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:15 [entrez] AID - 10.1007/s00216-026-06885-5 [pii] AID - 10.1007/s00216-026-06885-5 [doi] PST - aheadofprint SO - Anal Bioanal Chem. 2026 Oct 27. doi: 10.1007/s00216-026-06885-5. PMID- 42944889 OWN - NLM STAT- Publisher LR - 20261027 IS - 1432-0932 (Electronic) IS - 0940-6719 (Linking) DP - 2026 Oct 27 TI - Developing and validating a nomogram for predicting cervical disc resorption after posterior cervical single open-door laminoplasty: a retrospective cohort study of 266 cases. LID - 10.1007/s00586-026-10433-1 [doi] AB - PURPOSE: For patients with multilevel degenerative cervical myelopathy caused by cervical spinal stenosis involving >/= 3 intervertebral segments, cervical disc resorption (CDR) may be observed following posterior cervical single open-door laminoplasty (PCSOL). CDR has been associated with a lower observed rate of secondary anterior decompression; however, this association does not establish causality, and the predictive factors for CDR remain unclear. METHODS: Data from 266 patients who underwent PCSOL at the authors' institution between June 2016 and June 2022, were retrospectively analyzed. Least absolute shrinkage and selection operator (LASSO) regression was used to identify predictors of postoperative CDR and to construct a predictive model. Internal validation was performed using bootstrap resampling (n = 1000 iterations). The discriminative ability, calibration, and clinical utility of the model were assessed according to the area under the receiver operating characteristic curve (AUC), calibration curve, and decision curve analysis (DCA), respectively. RESULTS: CDR occurred in 122 of 266 patients (45.9%). LASSO regression identified 5 potential predictors: sex, age, course of disease, C2-7 Cobb angle, and T1 slope. A nomogram incorporating these factors was developed and demonstrated good discriminative ability (AUC 0.839 [95% confidence interval 0.791-0.886]). The calibration curve indicated good agreement between predicted and observed probabilities (Hosmer-Lemeshow test P-value = 0.415). DCA confirmed the clinical utility of this nomogram. CONCLUSION: For patients with multilevel degenerative cervical myelopathy involving >/= 3 segments, a nomogram capable of identifying patients at probability of CDR after PCSOL was developed and validated. This model provides clinicians with a theoretical basis for optimizing treatment strategies. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Yu, Dechen AU - Yu D AD - Department of Orthopedics, The First Affiliated Hospital of Air Force Medical University, Xi'an, China. FAU - Cao, Xiongfei AU - Cao X AD - Department of Orthopedics, The First Affiliated Hospital of Air Force Medical University, Xi'an, China. FAU - Su, Kangkang AU - Su K AD - Department of Orthopedics, The First Affiliated Hospital of Air Force Medical University, Xi'an, China. FAU - Li, Pan AU - Li P AD - Department of Orthopedics, The First Affiliated Hospital of Air Force Medical University, Xi'an, China. FAU - Li, Mo AU - Li M AD - Department of Orthopedics, The First Affiliated Hospital of Air Force Medical University, Xi'an, China. limo0729@hotmail.com. LA - eng PT - Journal Article DEP - 20261027 PL - Germany TA - Eur Spine J JT - European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society JID - 9301980 SB - IM OTO - NOTNLM OT - Cervical disc resorption OT - Cervical spinal stenosis OT - Degenerative cervical myelopathy OT - Nomogram OT - Posterior cervical single open-door laminoplasty OT - Retrospective cohort study COIS- Declarations. Conflict of interest: The authors declare no competing interests. Ethics approval and consent to participate: This retrospective study meeting the ethical standards of the Helsinki Declarations was approved by the author's hospital ethics committee (KY20252473-C-1). EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:15 PHST- 2026/05/03 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/09/24 00:00 [revised] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:15 [entrez] AID - 10.1007/s00586-026-10433-1 [pii] AID - 10.1007/s00586-026-10433-1 [doi] PST - aheadofprint SO - Eur Spine J. 2026 Oct 27. doi: 10.1007/s00586-026-10433-1. PMID- 42944876 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1420-908X (Electronic) IS - 1023-3830 (Linking) VI - 75 IP - 1 DP - 2026 Oct 27 TI - Biologic therapy in type 2 CRSwNP: from immunopathology to clinical management. LID - 251 [pii] LID - 10.1007/s00011-026-02399-z [doi] AB - BACKGROUND: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a heterogeneous inflammatory disorder in which type 2 (T2) inflammation represents the predominant endotype, characterized by eosinophilic infiltration and activation of IL-4, IL-5, and IL-13 pathways. Patients with T2-CRSwNP frequently present with asthma, NSAID-exacerbated respiratory disease, and a high risk of recurrence. Conventional management based on intranasal or systemic corticosteroids and endoscopic sinus surgery may relieve symptoms but often fails to achieve sustained disease control in patients with persistent T2 inflammation. PURPOSE: This review summarizes the immunopathology and risk stratification of T2-CRSwNP and discusses current evidence and practical considerations for biologic therapy within a structured clinical decision framework. CONCLUSIONS: Biologic therapies targeting IL-4Ralpha, IL-5/IL-5R, IgE, and TSLP have demonstrated efficacy in reducing polyp burden, improving symptoms, and decreasing systemic corticosteroid exposure. However, in the absence of robust head-to-head comparisons, optimal patient selection and defining sustainable long-term maintenance strategies remain clinical challenges. Integrating inflammatory profiles, disease burden, and structured follow-up may support individualized treatment and sustained disease control. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature Switzerland AG. FAU - He, Wei AU - He W AD - Department of ENT, The 3rd Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510630, China. FAU - Jiang, Yu AU - Jiang Y AD - Department of ENT, The 3rd Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510630, China. FAU - Ge, Rongrong AU - Ge R AD - Department of ENT, The 3rd Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510630, China. FAU - Wu, Shuo AU - Wu S AD - Department of ENT, The 3rd Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510630, China. Wush68@sysu.edu.cn. AD - Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument, School of Biomedical Engineering, Shenzhen Campus of Sun Yat-Sen University, Shenzhen, 518107, China. Wush68@sysu.edu.cn. LA - eng GR - No. QHYH202508/the Yuanhang Program of the Third Affiliated Hospital of Sun Yat-sen University/ PT - Journal Article PT - Review DEP - 20261027 PL - Switzerland TA - Inflamm Res JT - Inflammation research : official journal of the European Histamine Research Society ... [et al.] JID - 9508160 SB - IM MH - Humans MH - *Rhinosinusitis/immunology/therapy/drug therapy/pathology MH - *Nasal Polyps/immunology/therapy/drug therapy/pathology MH - Chronic Disease MH - *Biological Therapy OTO - NOTNLM OT - Biologic therapy OT - Chronic rhinosinusitis with nasal polyps OT - Nasal polyps OT - Targeted therapy OT - Type 2 inflammation COIS- Declarations. Conflict of interest: The authors declare that they have no conflict of interest. Consent to participate: Not applicable. Consent for publication: Not applicable. Ethical approval: Not applicable. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:14 PHST- 2026/05/31 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/05/31 00:00 [revised] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:14 [entrez] AID - 10.1007/s00011-026-02399-z [pii] AID - 10.1007/s00011-026-02399-z [doi] PST - epublish SO - Inflamm Res. 2026 Oct 27;75(1):251. doi: 10.1007/s00011-026-02399-z. PMID- 42944875 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1432-2242 (Electronic) IS - 0040-5752 (Print) IS - 0040-5752 (Linking) VI - 139 IP - 11 DP - 2026 Oct 27 TI - Stem rust resistance in North American spring wheat to Ug99 and other virulent pathotypes of Puccinia graminis f. sp. tritici. LID - 10.1007/s00122-026-05352-0 [doi] LID - 318 AB - North American spring wheat shows limited seedling resistance to a diverse set of exotic Pgt races but harbors valuable field resistance, including a strong locus for field resistance on chromosome 7AL. The emergence and spread of Ug99 and other races of Puccinia graminis f. sp. tritici (Pgt) with virulence to widely deployed resistance genes threaten global wheat production. This study evaluated North American spring wheat germplasm for seedling responses to four Pgt races (TTKSK, TTKSF+, TKTTF, and TRTTF) and for field response across five environments in Ethiopia and Kenya. Seedling evaluation revealed significant race-specific variation, with 82% of the lines exhibiting resistance to race TKTTF but very limited resistance to the other races. Gene postulation based on resistance patterns suggested a high frequency of Sr7a (~ 92%) in this panel, with only a few lines (~ 5%) carrying Sr8a, Sr11, and/or Sr31. Field evaluations in Ethiopia and Kenya demonstrated quantitative variation for field response, with several lines showing consistent field resistance. Association mapping identified 37 significant associations for seedling resistance, representing 17 unique genomic regions. For field response, 15 significant associations were detected, including a region on chromosome arm 7AL that was consistently detected across environments. Further analysis indicated that this locus confers adult-plant resistance (APR) and might be independent of known stem rust resistance genes located in the region. Hence, KASP assay was developed to enable marker-assisted selection and further investigation of this locus. This study highlights limited seedling resistance but identifies promising sources of field resistance against exotic Pgt races and provides valuable insights into the genetics underlying both seedling and field responses in North American germplasm. CI - (c) 2026. The Author(s). FAU - Gill, Harsimardeep S AU - Gill HS AD - Department of Agronomy and Plant Genetics, University of Minnesota, Saint Paul, MN, 55108, USA. FAU - Conley, Emily AU - Conley E AD - Department of Agronomy and Plant Genetics, University of Minnesota, Saint Paul, MN, 55108, USA. FAU - Ndung'u, Kimani Cyrus AU - Ndung'u KC AD - Food Crops Research Center, Kenya Agricultural and Livestock Research Organization (KALRO), Njoro, Kenya. FAU - Firpo, Pablo Olivera AU - Firpo PO AD - Cereal Disease Lab, United States Department of Agriculture-Agricultural Research Service, St. Paul, MN, 55108, USA. FAU - Brault, Charlotte AU - Brault C AD - Department of Agronomy and Plant Genetics, University of Minnesota, Saint Paul, MN, 55108, USA. FAU - Kosgey, Zennah AU - Kosgey Z AD - Food Crops Research Center, Kenya Agricultural and Livestock Research Organization (KALRO), Njoro, Kenya. FAU - Degete, Ashenafi Gemechu AU - Degete AG AD - Debre Zeit Agricultural Research Center, Ethiopian Institute of Agricultural Research, Bishoftu, Ethiopia. FAU - Bhavani, Sridhar AU - Bhavani S AD - International Maize and Wheat Improvement Center (CIMMYT), ICRAF Campus, United Nations Avenue, P.O. Box 1041-00621, Gigiri, Nairobi, Kenya. FAU - Bajgain, Prabin AU - Bajgain P AD - U.S. Dairy Forage Research Center, United States Department of Agriculture-Agricultural Research Service, Madison, WI, 53706, USA. FAU - Rouse, Matthew N AU - Rouse MN AD - United States Department of Agriculture-Agricultural Research Service, Sugarcane Field Station, Canal Point, FL, 33438, USA. AD - Department of Plant Pathology, University of Florida, Gainesville, FL, 32611, USA. FAU - Jin, Yue AU - Jin Y AD - Department of Plant Pathology, University of Minnesota, Saint Paul, MN, 55108, USA. FAU - Anderson, James A AU - Anderson JA AD - Department of Agronomy and Plant Genetics, University of Minnesota, Saint Paul, MN, 55108, USA. ander319@umn.edu. LA - eng GR - 58-5062-3-008/Agricultural Research Service/ GR - BFS-G-11-00002/United States Agency for International Development/ PT - Journal Article DEP - 20261027 PL - Germany TA - Theor Appl Genet JT - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JID - 0145600 RN - 0 (Genetic Markers) RN - Puccinia graminis SB - IM MH - *Triticum/microbiology/genetics MH - *Plant Diseases/microbiology/genetics MH - *Disease Resistance/genetics MH - *Puccinia/pathogenicity MH - Chromosome Mapping MH - Virulence MH - Seedlings/microbiology/genetics MH - Phenotype MH - Quantitative Trait Loci MH - *Basidiomycota/pathogenicity MH - Genetic Markers MH - Genotype MH - Ethiopia PMC - PMC13714084 COIS- Declarations. Conflict of interest: The authors declare no conflict of interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 12:14 PHST- 2026/04/15 00:00 [received] PHST- 2026/08/09 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:14 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 10.1007/s00122-026-05352-0 [pii] AID - 5352 [pii] AID - 10.1007/s00122-026-05352-0 [doi] PST - epublish SO - Theor Appl Genet. 2026 Oct 27;139(11):318. doi: 10.1007/s00122-026-05352-0. PMID- 42944869 OWN - NLM STAT- Publisher LR - 20261027 IS - 1435-702X (Electronic) IS - 0721-832X (Linking) DP - 2026 Oct 27 TI - Twelve-month outcomes of a tube-in-tube PreserFlo MicroShunt-Baerveldt glaucoma implant connection as a salvage procedure in refractory glaucoma: a pilot series. LID - 10.1007/s00417-026-07576-8 [doi] AB - PURPOSE: To report 12-month outcomes of a tube-in-tube connection between an existing PreserFlo MicroShunt (PFMS) and a Baerveldt glaucoma implant (BGI) used as a salvage procedure after PFMS failure or conjunctival/tube-related complications. METHODS: Retrospective, single-center, consecutive case series of 6 pseudophakic eyes of 6 patients. A 350-mm(2) BGI was connected to the extraocular segment of the PFMS, which was retained as the anterior chamber entry and flow-restricting conduit while the Baerveldt plate served as a posterior reservoir. Outcome parameters at 12 months were intraocular pressure (IOP), number of topical antiglaucoma medications, surgical success, complications, and reinterventions. RESULTS: Mean IOP decreased from 29.8 +/- 15.0 mmHg preoperatively to 13.7 +/- 4.6 mmHg at 12 months. Mean topical medications decreased from 3.0 +/- 1.1 to 1.7 +/- 1.9; systemic acetazolamide, used in 5 of 6 eyes preoperatively, was discontinued in all eyes. Qualified and complete success were achieved in 2 of 6 (33%) and 1 of 6 eyes (17%), respectively; 4 of 6 eyes were within their individualized target range. No clinically significant hypotony occurred. When the 2 eyes that underwent additional IOP-lowering surgery were excluded, the mean 12-month IOP in the remaining 4 eyes was 13.0 mmHg. Reinterventions comprised bleb revision with device exchange in 1 eye, scleral patch graft for tube exposure in 1 eye, and conjunctival reconstruction followed by micropulse cyclophotocoagulation in 1 eye. CONCLUSION: The tube-in-tube PFMS-BGI connection lowered IOP and medication burden at 12 months while preserving the original anterior chamber entry, but composite success was modest and conjunctival or tube complications were common. The technique should be reserved for selected salvage cases and is distinct from primary combined PFMS-BGI implantation. CI - (c) 2026. The Author(s). FAU - Birjandi, Alireza AU - Birjandi A AUID- ORCID: 0009-0005-7271-5536 AD - Department of Ophthalmology, Hannover Medical School, Hannover, Germany. helalbirjandi.alireza@mh-hannover.de. FAU - Unterlauft, Jan-Darius AU - Unterlauft JD AUID- ORCID: 0000-0003-1469-0412 AD - Department of Ophthalmology, University of Leipzig, Leipzig, Germany. FAU - Liegl, Constance AU - Liegl C AUID- ORCID: 0000-0002-9714-2851 AD - Department of Ophthalmology, Klinikum Dortmund, University Witten/Herdecke, Dortmund, Germany. FAU - Framme, Carsten AU - Framme C AUID- ORCID: 0000-0001-8685-176X AD - Department of Ophthalmology, Hannover Medical School, Hannover, Germany. FAU - Panidou-Marschelke, Ermioni AU - Panidou-Marschelke E AUID- ORCID: 0009-0008-9409-4023 AD - Department of Ophthalmology, Hannover Medical School, Hannover, Germany. panermioni@googlemail.com. AD - Regensburg Eye Institute, Regensburg, Germany. panermioni@googlemail.com. LA - eng PT - Journal Article DEP - 20261027 PL - Germany TA - Graefes Arch Clin Exp Ophthalmol JT - Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie JID - 8205248 SB - IM OTO - NOTNLM OT - Baerveldt glaucoma implant OT - Glaucoma drainage device OT - PreserFlo MicroShunt OT - Refractory glaucoma OT - Surgical salvage OT - Tube-in-tube connection COIS- Declarations. Ethics approval: This study was performed in line with the principles of the Declaration of Helsinki. Approval was granted by the Ethics Committee of Hannover Medical School (approval number 11845-BO-K-2025; date 11 June 2025). Consent to participate: The requirement for written informed consent was waived by the Ethics Committee of Hannover Medical School owing to the retrospective design of the study. Consent to publish: Written informed consent for publication of ocular and surgical images was obtained. Competing interests: The authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:13 PHST- 2026/06/14 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:13 [entrez] AID - 10.1007/s00417-026-07576-8 [pii] AID - 10.1007/s00417-026-07576-8 [doi] PST - aheadofprint SO - Graefes Arch Clin Exp Ophthalmol. 2026 Oct 27. doi: 10.1007/s00417-026-07576-8. PMID- 42944866 OWN - NLM STAT- Publisher LR - 20261027 IS - 1433-0458 (Electronic) IS - 0017-6192 (Linking) DP - 2026 Oct 27 TI - [Update on immunotherapy in head and neck cancer : New aspects from the ASCO annual meeting 2026]. LID - 10.1007/s00106-026-01823-4 [doi] AB - BACKGROUND: Immune checkpoint inhibitors (ICIs) targeting PD-1/PD-L1 have reshaped the treatment of head and neck squamous cell carcinoma, but durable benefit remains limited to selected subgroups. New perioperative and palliative strategies, including bispecific and T‑cell receptor (TCR)-based approaches, aim to overcome immunosuppressive barriers and improve long-term control. OBJECTIVE: Immunotherapy trials in head and neck cancer presented at the 2026 American Society of Clinical Oncology (ASCO) Annual Meeting were systematically reviewed and their impact on current standards and future strategies in frontline and recurrent/metastatic settings was analyzed. MATERIALS AND METHODS: Clinical trials of PD-1/PD-L1-based therapies, bispecific antibodies, and innovative strategies in curative and palliative head and neck cancer were reviewed, based on abstracts and presentations at ASCO 2026. RESULTS: In the curative setting, a perioperative and adjuvant PD-1-based strategy with pembrolizumab provided consistent survival benefits and reduced pathological high-risk features while preserving surgical radicality. Neoadjuvant bispecific PD-1/vascular endothelial growth factor (VEGF) blockade combined with chemotherapy achieved high response rates and organ-preserving resections, whereas adjuvant CD40-targeted vaccination in human papillomavirus (HPV)16-positive oropharyngeal head and neck squamous cell carcinoma induced robust, durable T‑cell responses. In the recurrent/metastatic setting, PD-1-based combinations with targeted agents improved tumor response, disease control, and survival. Bispecific epidermal growth factor receptor (EGFR)-directed antibodies and the TCR-based bispecific T‑cell engaging receptor (TCER) IMA401 showed meaningful activity after PD‑1 failure. CONCLUSION: These data support the expansion of immuno-oncologic concepts into perioperative and palliative settings. Bispecific and TCR-based strategies signal a shift from conventional ICI monotherapy toward more differentiated, mechanism-driven combination approaches. CI - (c) 2026. The Author(s), under exclusive licence to Springer Medizin Verlag GmbH, ein Teil von Springer Nature. FAU - Brand, Matthias AU - Brand M AUID- ORCID: 0009-0007-9722-8771 AD - Klinik fur Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Universitatsklinik Ulm, Ulm, Deutschland. matthias.brand@uniklinik-ulm.de. AD - Kopf-Hals-Tumorzentrum des Comprehensive Cancer Center Ulm, Universitatsklinik Ulm, Ulm, Deutschland. matthias.brand@uniklinik-ulm.de. FAU - Hoffmann, Thomas K AU - Hoffmann TK AD - Klinik fur Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Universitatsklinik Ulm, Ulm, Deutschland. AD - Kopf-Hals-Tumorzentrum des Comprehensive Cancer Center Ulm, Universitatsklinik Ulm, Ulm, Deutschland. FAU - Laban, Simon AU - Laban S AD - Klinik fur Hals-Nasen-Ohrenheilkunde und Kopf-Halschirurgie, Universitatsklinik Ulm, Ulm, Deutschland. AD - Kopf-Hals-Tumorzentrum des Comprehensive Cancer Center Ulm, Universitatsklinik Ulm, Ulm, Deutschland. LA - ger PT - English Abstract PT - Journal Article PT - Review TT - Immuntherapie-Update fur Kopf-Hals-Tumoren : Erkenntnisse der ASCO-Jahresversammlung 2026. DEP - 20261027 PL - Germany TA - HNO JT - HNO JID - 2985099R SB - IM OTO - NOTNLM OT - Head and neck squamous cell carcinoma OT - Human papilloma virus OT - Immune checkpoint inhibitors OT - Immunotherapy OT - Neoadjuvant immunotherapy COIS- Einhaltung ethischer Richtlinien. Interessenkonflikt: S. Laban: Honoraria: Merck Sharp & Dohme; Beratung/Advisory Boards: Bristol Myers Squibb, Bohringer Ingelheim, Glaxo Smith Kline, Merck Sharp & Dohme; Reisekosten: Merck Healthcare (ESMO 2024); Patente: Peptides for antigen-specific immunotherapy of head and neck cancer or other cancers (EP2023/071063, patent pending). T.K. Hoffmann: Merck Sharp & Dohme. M. Brand gibt an, dass kein Interessenkonflikt besteht. Fur diesen Beitrag wurden von den Autor/-innen keine Studien an Menschen oder Tieren durchgefuhrt. Fur die aufgefuhrten Studien gelten die jeweils dort angegebenen ethischen Richtlinien. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:13 PHST- 2026/07/31 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:13 [entrez] AID - 10.1007/s00106-026-01823-4 [pii] AID - 10.1007/s00106-026-01823-4 [doi] PST - aheadofprint SO - HNO. 2026 Oct 27. doi: 10.1007/s00106-026-01823-4. PMID- 42944865 OWN - NLM STAT- Publisher LR - 20261027 IS - 1432-1327 (Electronic) IS - 0949-8257 (Linking) DP - 2026 Oct 27 TI - Ruthenium(II) coordinated with diphenylphosphine and 2-mercaptothiazoline as a selective antiproliferative agent in breast cancer cells. LID - 10.1007/s00775-026-02178-5 [doi] AB - Breast cancer is the most common malignant neoplasm among women and exhibits high molecular heterogeneity, which directly influences therapeutic response and clinical outcomes. Despite advances in targeted therapies and immunotherapies, tumor recurrence, resistance to conventional treatments, and metastatic potential reinforce the need for more selective and effective pharmacological strategies. In this context, metal complexes, particularly ruthenium(II) derivatives, have attracted considerable attention due to their antitumor profile, greater selectivity, and lower systemic toxicity. Therefore, in the present study, the antineoplastic activity of the RuMTZ complex (2-mercaptothiazoline-di-1,2-bis(diphenylphosphine)ethane-ruthenium(II)) was evaluated in three breast cancer cell lines (MDA-MB-231, MDA-MB-468 and MCF7) and in one non-tumor breast cell line (MCF10A). RuMTZ exhibited greater cytotoxicity against human MDA-MB-468 cells, as demonstrated by cell viability (IC(50) = 1.8 microM) and clonogenic (IC(50) = 0.08 microM) assays, associated with higher selectivity indexes (3.89 and 3.75, respectively). Further studies demonstrated that RuMTZ inhibited cell migration by up to 62.76% and induced cell-cycle arrest in the G0/G1 phase, accompanied by a reduction in the G2/M population. Furthermore, the ruthenium complex induced apoptotic cell death, as observed by flow cytometry and increased levels of cleaved caspase-3 by Western blot. The genotoxic effect of RuMTZ was evidenced by the comet assay and increased gammaH2AX expression levels. Taken together, these findings demonstrate that RuMTZ exhibits promising antineoplastic activity against breast cancer cells, associated with cell-cycle arrest, induction of DNA damage, and apoptosis. CI - (c) 2026. The Author(s). FAU - de Melo, Matheus Reis Santos AU - de Melo MRS AUID- ORCID: 0000-0001-5475-2492 AD - Laboratory of Mutagenesis, University of Franca, Franca, Sao Paulo, 14404-600, Brazil. FAU - Ribeiro, Arthur Barcelos AU - Ribeiro AB AUID- ORCID: 0000-0002-4056-9571 AD - Laboratory of Mutagenesis, University of Franca, Franca, Sao Paulo, 14404-600, Brazil. arthur1_@hotmail.com. FAU - de Melo Junqueira, Marcela AU - de Melo Junqueira M AUID- ORCID: 0000-0002-4636-4446 AD - Laboratory of Mutagenesis, University of Franca, Franca, Sao Paulo, 14404-600, Brazil. FAU - Silveira, Natalia Nascimento AU - Silveira NN AUID- ORCID: 0000-0003-2672-2880 AD - Laboratory of Mutagenesis, University of Franca, Franca, Sao Paulo, 14404-600, Brazil. AD - Laboratory of Genetics and Molecular Biology, University of Franca, Franca, Sao Paulo, 14404-600, Brazil. FAU - Dos Santos, Raquel Alves AU - Dos Santos RA AUID- ORCID: 0000-0002-0075-2099 AD - Laboratory of Mutagenesis, University of Franca, Franca, Sao Paulo, 14404-600, Brazil. AD - Laboratory of Genetics and Molecular Biology, University of Franca, Franca, Sao Paulo, 14404-600, Brazil. FAU - Palmeira-Mello, Marcos V AU - Palmeira-Mello MV AUID- ORCID: 0000-0003-2550-5315 AD - Department of Chemistry, Federal University of Sao Carlos, Sao Carlos, Sao Paulo, 13565-905, Brazil. FAU - Batista, Alzir Azevedo AU - Batista AA AUID- ORCID: 0000-0002-4671-2754 AD - Department of Chemistry, Federal University of Sao Carlos, Sao Carlos, Sao Paulo, 13565-905, Brazil. FAU - Tavares, Denise Crispim AU - Tavares DC AUID- ORCID: 0000-0003-4646-5914 AD - Laboratory of Mutagenesis, University of Franca, Franca, Sao Paulo, 14404-600, Brazil. denisecrispim2001@yahoo.com. LA - eng PT - Journal Article DEP - 20261027 PL - Germany TA - J Biol Inorg Chem JT - Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry JID - 9616326 SB - IM OTO - NOTNLM OT - Cleaved caspase-3 OT - Mercapto groups OT - Metallodrug OT - gammaH2AX COIS- Declarations. Conflict of interest: The authors declare that they have no known competing interests in this paper. Ethics, Consent to Participate, and Consent to Publish: Not applicable. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:13 PHST- 2026/06/25 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:13 [entrez] AID - 10.1007/s00775-026-02178-5 [pii] AID - 10.1007/s00775-026-02178-5 [doi] PST - aheadofprint SO - J Biol Inorg Chem. 2026 Oct 27. doi: 10.1007/s00775-026-02178-5. PMID- 42944864 OWN - NLM STAT- Publisher LR - 20261027 IS - 1432-1971 (Electronic) IS - 0172-0643 (Linking) DP - 2026 Oct 27 TI - Clinical Effects of Sodium-Glucose Cotransporter 2 Inhibitors on Children with Single Ventricle Congenital Heart Disease: A Single-Center Experience. LID - 10.1007/s00246-026-04532-5 [doi] AB - This study evaluates the impact of sodium-glucose cotransporter 2 inhibitors (SGLT2i) on pediatric patients with single ventricle (SV) heart disease, using invasive and non-invasive markers of cardiac function. We reviewed patients (< 21 years) with SV physiology who received >/= 90 days SGLT2i at our center from 2020 to 2025. Baseline laboratory, echocardiographic, and hemodynamic data were recorded and compared to follow up data after >/= 90 days on SGLT2i. Hospitalizations before and after SGLT2i were also analyzed. Seventy-seven patients with median age 10.5 years (range 47 days-19.9 years) had median treatment duration of 386 days (IQR 218-853). Among 62 patients (81%) with echocardiographic data (Fontan [39], Glenn [18], Stage 1 [5]) there was a significant decrease in median qualitative AVVR score from mild-moderate to mild (p = 0.01). Median BNP decreased from 84 pg/mL (IQR 18-274) to 42 pg/mL (IQR 18-105) (p = 0.02) and mean hemoglobin increased from 14.4 +/- 2.5 g/dL to 15.5 +/- 2.4 g/dL (p < 0.01). Ten patients had hemodynamic data without significant change in mean pulmonary artery pressure from 17 to 16 mmHg (p = 0.168) or ventricular end-diastolic pressure from 10 to 11 mmHg (p = 0.275). More patients had > 1 hospitalizations for heart failure during the 6-months prior to SGLT2i compared to the 6-months following initiation, with an odds ratio of 4.5 (95% CI: 1.6-13.7, p < 0.01). Overall, we identified significant reductions in AVVR, BNP, and hospitalizations for heart failure after >/= 90 days of therapy. Further larger scale studies are needed to determine if SGLT2i will affect clinical outcomes in the pediatric SV population. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Canova, Alexander O AU - Canova AO AUID- ORCID: 0009-0005-3233-2418 AD - School of Medicine, University of Washington, Seattle, WA, USA. Alexander.Canova@seattlechildrens.org. AD - Heart Center, Seattle Children's Hospital, Seattle, WA, USA. Alexander.Canova@seattlechildrens.org. FAU - Newland, David M AU - Newland DM AUID- ORCID: 0000-0003-0037-8065 AD - Department of Pharmacy, Seattle Children's Hospital, Seattle, WA, USA. AD - School of Pharmacy, University of Washington, Seattle, WA, USA. FAU - Albers, Erin L AU - Albers EL AUID- ORCID: 0000-0002-3072-4195 AD - School of Medicine, University of Washington, Seattle, WA, USA. AD - Heart Center, Seattle Children's Hospital, Seattle, WA, USA. FAU - Bouska, Erin S AU - Bouska ES AD - School of Medicine, University of Washington, Seattle, WA, USA. AD - Heart Center, Seattle Children's Hospital, Seattle, WA, USA. FAU - Hartje-Dunn, Christina L AU - Hartje-Dunn CL AUID- ORCID: 0009-0001-4884-9957 AD - School of Medicine, University of Washington, Seattle, WA, USA. AD - Heart Center, Seattle Children's Hospital, Seattle, WA, USA. FAU - Iqbal, Mehreen S AU - Iqbal MS AD - School of Medicine, University of Washington, Seattle, WA, USA. AD - Heart Center, Seattle Children's Hospital, Seattle, WA, USA. FAU - Kemna, Mariska S AU - Kemna MS AUID- ORCID: 0000-0001-9484-3006 AD - School of Medicine, University of Washington, Seattle, WA, USA. AD - Heart Center, Seattle Children's Hospital, Seattle, WA, USA. FAU - Law, Yuk M AU - Law YM AUID- ORCID: 0000-0002-4282-4812 AD - School of Medicine, University of Washington, Seattle, WA, USA. AD - Heart Center, Seattle Children's Hospital, Seattle, WA, USA. FAU - Kerr, Sarah M AU - Kerr SM AD - School of Medicine, University of Washington, Seattle, WA, USA. AD - Heart Center, Seattle Children's Hospital, Seattle, WA, USA. FAU - Wisotzkey, Bethany L AU - Wisotzkey BL AUID- ORCID: 0000-0002-8422-1470 AD - School of Medicine, University of Washington, Seattle, WA, USA. AD - Heart Center, Seattle Children's Hospital, Seattle, WA, USA. FAU - Hong, Borah J AU - Hong BJ AUID- ORCID: 0000-0002-5294-6821 AD - School of Medicine, University of Washington, Seattle, WA, USA. AD - Heart Center, Seattle Children's Hospital, Seattle, WA, USA. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Pediatr Cardiol JT - Pediatric cardiology JID - 8003849 SB - IM OTO - NOTNLM OT - Congenital heart disease OT - Heart failure OT - Single ventricle OT - Sodium-glucose cotransporter 2 inhibitor COIS- Declarations. Competing Interests: The authors declare no competing interests. Informed Consent: The need for informed consent was waived for this study of deidentified data. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:13 PHST- 2026/08/26 00:00 [received] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:13 [entrez] AID - 10.1007/s00246-026-04532-5 [pii] AID - 10.1007/s00246-026-04532-5 [doi] PST - aheadofprint SO - Pediatr Cardiol. 2026 Oct 27. doi: 10.1007/s00246-026-04532-5. PMID- 42944862 OWN - NLM STAT- Publisher LR - 20261027 IS - 1432-1971 (Electronic) IS - 0172-0643 (Linking) DP - 2026 Oct 27 TI - Outcomes of Handmade Tri-leaflet ePTFE Valved Conduits for RVOT Reconstruction: A Propensity-Matched Analysis Compared with PVR. LID - 10.1007/s00246-026-04533-4 [doi] AB - We aimed to evaluate the long-term durability and valve function of handmade expanded polytetrafluoroethylene (ePTFE) valved conduits of different calibers and compare their outcomes with those of bioprosthetic pulmonary valve replacement (PVR). We retrospectively reviewed 100 patients undergoing RVOT reconstruction between 2007 and 2025: 66 with ePTFE conduits and 34 with bioprosthetic valves. Patients were categorized into small-conduit, large-conduit, and PVR groups. Propensity score matching was performed to adjust for baseline differences. Mean age was 1.6 +/- 1.8, 11.2 +/- 6.5, and 26.3 +/- 16.9 years in the small-conduit, large-conduit, and PVR groups, respectively (p < 0.0001). Ten-year survival was similar among groups (100%, 100%, and 97%; p = 0.19). In contrast, the small-conduit group showed significantly lower freedom from RVOT reoperation than the large-conduit and PVR groups (53%, 85%, and 93%; p < 0.001), driven by progressive pulmonary stenosis (10-year freedom from moderate pulmonary stenosis: 9%, 55%, and 58%; p = 0.01). Freedom from moderate pulmonary regurgitation remained comparable (80%, 94%, and 85%; p = 0.37). In the matched cohort, conduit and PVR groups demonstrated similar freedom from reoperation (94% vs. 89%; p = 0.34), with numerically better valve function in the conduit group. In conclusion, large-caliber handmade ePTFE valved conduits demonstrated favorable long-term durability, with outcomes comparable to PVR in adolescent- and adult-sized patients. They may be a reasonable option for selected younger patients requiring redo RVOT reconstruction. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Masaki, Naoki AU - Masaki N AUID- ORCID: 0000-0002-0198-6495 AD - Department of Cardiovascular Surgery, Iwate Medical University, 2-1-1 Idaidori, Yahaba-cho, Shiwa-gun, Iwate, 028-3695, Japan. masaknao@iwate-med.ac.jp. FAU - Koizumi, Junichi AU - Koizumi J AUID- ORCID: 0000-0002-3940-8957 AD - Department of Cardiovascular Surgery, Iwate Medical University, 2-1-1 Idaidori, Yahaba-cho, Shiwa-gun, Iwate, 028-3695, Japan. jkoizumi@iwate-med.ac.jp. FAU - Yamazaki, Yuya AU - Yamazaki Y AD - Department of Cardiovascular Surgery, Iwate Medical University, 2-1-1 Idaidori, Yahaba-cho, Shiwa-gun, Iwate, 028-3695, Japan. FAU - Edaki, Daichi AU - Edaki D AD - Department of Cardiovascular Surgery, Iwate Medical University, 2-1-1 Idaidori, Yahaba-cho, Shiwa-gun, Iwate, 028-3695, Japan. FAU - Saito, Kanchi AU - Saito K AD - Division of Pediatric Cardiology, Department of Pediatrics, Iwate Medical University, Iwate, Japan. FAU - Sato, Akira AU - Sato A AD - Division of Pediatric Cardiology, Department of Pediatrics, Iwate Medical University, Iwate, Japan. FAU - Takizawa, Yurie AU - Takizawa Y AD - Division of Pediatric Cardiology, Department of Pediatrics, Iwate Medical University, Iwate, Japan. FAU - Nakano, Satoshi AU - Nakano S AD - Division of Pediatric Cardiology, Department of Pediatrics, Iwate Medical University, Iwate, Japan. FAU - Saiki, Hirofumi AU - Saiki H AUID- ORCID: 0000-0002-6543-8981 AD - Division of Pediatric Cardiology, Department of Pediatrics, Iwate Medical University, Iwate, Japan. FAU - Ikai, Akio AU - Ikai A AD - Department of Cardiovascular Surgery, Iwate Medical University, 2-1-1 Idaidori, Yahaba-cho, Shiwa-gun, Iwate, 028-3695, Japan. FAU - Kin, Hajime AU - Kin H AUID- ORCID: 0000-0002-2701-8257 AD - Department of Cardiovascular Surgery, Iwate Medical University, 2-1-1 Idaidori, Yahaba-cho, Shiwa-gun, Iwate, 028-3695, Japan. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Pediatr Cardiol JT - Pediatric cardiology JID - 8003849 SB - IM OTO - NOTNLM OT - Handmade valved conduit OT - Pulmonary atresia OT - Pulmonary valve replacement OT - Rastelli type operation OT - Right ventricular outflow tract reconstruction OT - Tetralogy of Fallot COIS- Declarations. Competing Interests: The authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:13 PHST- 2026/08/16 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:13 [entrez] AID - 10.1007/s00246-026-04533-4 [pii] AID - 10.1007/s00246-026-04533-4 [doi] PST - aheadofprint SO - Pediatr Cardiol. 2026 Oct 27. doi: 10.1007/s00246-026-04533-4. PMID- 42944858 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1432-0827 (Electronic) IS - 0171-967X (Linking) VI - 117 IP - 1 DP - 2026 Oct 27 TI - Comparative Effectiveness of Individual Angiotensin Receptor Blockers on Osteoporosis and Osteoporotic Fracture Risk: A Nationwide Active-Comparator New-User Cohort Study. LID - 170 [pii] LID - 10.1007/s00223-026-01630-7 [doi] AB - Osteoporosis is a major cause of morbidity and fragility fractures in older adults, and increasing evidence suggests that antihypertensive therapies may influence skeletal health beyond blood pressure control. Although angiotensin receptor blockers (ARBs) have been associated with favorable skeletal outcomes, whether clinically relevant heterogeneity exists among individual ARBs remains unclear. Telmisartan possesses distinctive pharmacological properties, including partial peroxisome proliferator-activated receptor-gamma (PPAR-gamma) agonistic activity, which may confer additional effects on bone metabolism. This study evaluated whether skeletal outcomes differ between telmisartan and other ARBs using an active-comparator new-user design. To evaluate whether clinically relevant differences in osteoporosis and osteoporotic fracture risk exist between telmisartan and other ARBs using Taiwan's Health and Welfare Data Science Center (HWDC) Database. Using Longitudinal Generation Tracking Database (LGTD) housed within the Health and Welfare Data Science Center (HWDC), we conducted a nationwide active-comparator new-user cohort study. Hypertensive patients aged 51-80 years who newly initiated telmisartan or another ARB between 2002 and 2021 were identified. Propensity score matching (1:4) was performed to balance baseline characteristics. The primary outcome was a composite of incident osteoporosis or osteoporotic fracture and time to first osteoporotic fracture was additionally evaluated as a secondary outcome. Adjusted hazard ratios (aHRs) and 95% confidence intervals (CIs) were estimated using Cox proportional hazards models. During a mean follow-up of 4.35 +/- 4.12 years, telmisartan use was associated with a lower risk of osteoporosis or osteoporotic fracture compared with other ARBs (adjusted HR, 0.65; 95% CI, 0.53-0.79). In analyses of individual outcomes, telmisartan use was associated with lower risks of osteoporosis (adjusted HR, 0.60; 95% CI, 0.43-0.84) and hip fracture (adjusted HR, 0.37; 95% CI, 0.19-0.70). In the secondary fracture-only analysis, telmisartan use was associated with a lower risk of first osteoporotic fracture (adjusted HR, 0.71; 95% CI, 0.57-0.90). Compared with other ARBs, telmisartan use was associated with a lower risk of osteoporosis and osteoporotic fractures in this nationwide cohort. These findings suggest that clinically relevant heterogeneity may exist within the ARB class and that antihypertensive drug selection may influence skeletal outcomes beyond blood pressure control. Further prospective studies are warranted to confirm these findings. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Wu, Hao-Ming AU - Wu HM AD - Cardiology Department, Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan. AD - College of Medicine, Institute of Medical Science and Technology, National Sun Yat-sen University, No. 70, Lienhai Road, Kaohsiung, 804, Taiwan (R.O.C.). FAU - Wu, Wen-Tung AU - Wu WT AD - Department of Pharmacy Practice, Tri-Service General Hospital, National Defense Medical University, Taipei, Taiwan. AD - College of Pharmacy, National Defense Medical University, Taipei, Taiwan. FAU - Tsai, Chun-Teng AU - Tsai CT AD - Department of Medical Research, Tri-Service General Hospital, National Defense Medical University, Taipei, Taiwan. FAU - Chien, Wu-Chien AU - Chien WC AD - Department of Medical Research, Tri-Service General Hospital, National Defense Medical University, Taipei, Taiwan. FAU - Chou, Yu-Ching AU - Chou YC AD - Graduate Institute of Long-Term Care, College of Health and Welfare, MacKay Medical University, New Taipei City, Taiwan. FAU - Lin, Ming-Hsun AU - Lin MH AUID- ORCID: 0000-0003-4169-921X AD - Division of Endocrinology and Metabolism, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical University, No. 325, Section 2, Cheng-Kung Road, Neihu Dist., Taipei, 114, Taiwan (R.O.C.). tim6801@msn.com. FAU - Chuang, Cheng-Hsin AU - Chuang CH AD - College of Medicine, Institute of Medical Science and Technology, National Sun Yat-sen University, No. 70, Lienhai Road, Kaohsiung, 804, Taiwan (R.O.C.). chchuang@imst.nsysu.edu.tw. LA - eng PT - Comparative Study PT - Journal Article DEP - 20261027 PL - United States TA - Calcif Tissue Int JT - Calcified tissue international JID - 7905481 RN - 0 (Angiotensin Receptor Antagonists) RN - U5SYW473RQ (Telmisartan) SB - IM MH - Humans MH - *Osteoporotic Fractures/epidemiology/prevention & control MH - Female MH - *Osteoporosis/drug therapy/epidemiology MH - *Angiotensin Receptor Antagonists/therapeutic use MH - *Telmisartan/therapeutic use MH - Taiwan/epidemiology MH - Aged MH - Male MH - Cohort Studies MH - Aged, 80 and over MH - Middle Aged MH - Hypertension/drug therapy MH - Risk Factors OTO - NOTNLM OT - Angiotensin receptor blockers OT - Comparative effectiveness OT - Osteoporosis OT - Osteoporotic fracture OT - Pharmacoepidemiology COIS- Declarations. Conflict of interest: The authors declare no conflict of interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:13 PHST- 2026/07/17 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:13 [entrez] AID - 10.1007/s00223-026-01630-7 [pii] AID - 10.1007/s00223-026-01630-7 [doi] PST - epublish SO - Calcif Tissue Int. 2026 Oct 27;117(1):170. doi: 10.1007/s00223-026-01630-7. PMID- 42944849 OWN - NLM STAT- Publisher LR - 20261027 IS - 1435-1463 (Electronic) IS - 0300-9564 (Linking) DP - 2026 Oct 27 TI - Altered developmental trajectories in neurodevelopmental disorders: mechanisms, human models and translational perspectives. LID - 10.1007/s00702-026-03315-9 [doi] AB - Neurodevelopmental disorders (NDDs) are clinically and genetically heterogeneous conditions often studied through diagnostic categories, causal genes, synaptic dysfunction or end-stage phenotypes. Although these approaches remain essential, they do not fully capture the developmental nature of NDD pathophysiology. This review proposes a trajectory-first framework in which NDDs are interpreted as disorders of altered developmental progression. From this perspective, the key question is not only which gene, pathway, cell type, or synapse is affected, but when, where and how neurodevelopment begins to diverge. We discuss mechanisms of trajectory divergence, including altered developmental timing, cell-state and lineage disruption, selective cellular vulnerability, epigenetic and signaling dysregulation, cytoskeletal and morphogenetic abnormalities, synaptic maturation defects, excitability changes and network-level alterations. We then examine how human systems, including fetal and post-mortem atlases, iPSC-derived models, cortical organoids, assembloids, functional readouts and patient-level phenotypes, can be integrated to reconstruct developmental trajectories across biological scales. Representative NDD genes and syndromes illustrate that distinct divergence points may later converge on shared functional or clinical outcomes, including altered excitation/inhibition balance, abnormal network activity, epilepsy, language delay, intellectual disability, autism spectrum disorder features or motor dysfunction. Finally, we discuss limitations and perspectives for trajectory-based stratification and biomarker discovery. Rather than acting as static diagnostic readouts, future biomarkers should aim to capture developmental state, timing, divergence, convergence, compensation and response to intervention. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature. FAU - Desprez, Florence AU - Desprez F AD - Universite de Tours, INSERM, Imaging Brain & Neuropsychiatry iBraiN U1253, 37032, Tours, France. FAU - Ung, Devina C AU - Ung DC AUID- ORCID: 0000-0002-5703-7002 AD - Universite de Tours, INSERM, Imaging Brain & Neuropsychiatry iBraiN U1253, 37032, Tours, France. devina.ung@univ-tours.fr. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Austria TA - J Neural Transm (Vienna) JT - Journal of neural transmission (Vienna, Austria : 1996) JID - 9702341 SB - IM OTO - NOTNLM OT - Cortical development OT - Developmental trajectories OT - Human iPSC models OT - Neurodevelopmental disorders OT - Organoids OT - Translational stratification COIS- Declarations. Conflict of interest: The authors declare that they have no competing financial or non-financial interests related to this work. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:12 PHST- 2026/05/31 00:00 [received] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:12 [entrez] AID - 10.1007/s00702-026-03315-9 [pii] AID - 10.1007/s00702-026-03315-9 [doi] PST - aheadofprint SO - J Neural Transm (Vienna). 2026 Oct 27. doi: 10.1007/s00702-026-03315-9. PMID- 42944847 OWN - NLM STAT- Publisher LR - 20261027 IS - 1435-1463 (Electronic) IS - 0300-9564 (Linking) DP - 2026 Oct 27 TI - Closing the neurology-psychiatry gap in tardive dyskinesia care in Asia: expert recommendations for an educational roadmap to improve prevention, recognition, and shared care. LID - 10.1007/s00702-026-03318-6 [doi] AB - Tardive dyskinesia (TD) remains an under-recognised medication safety issue in many Asian countries, compounded by fragmented care between psychiatry and neurology and underpinned by a lack of cross-discipline professional education. The multidisciplinary expert recommendations proposed here have been developed jointly by a panel of psychiatrists and neurologists representing selected Asian healthcare settings (Thailand, Japan, Korea and China), and suggest a practical, structured education and competency framework to help close this gap and improve multidisciplinary management. The recommendations emphasise the need for collaborative educational initiatives focused on rational antipsychotic prescribing, proactive monitoring, standardised assessment and diagnosis, patient and caregiver education, and timely specialist referral. The proposed joint neurology-psychiatry educational framework is informed by real-world expert perspectives and spans undergraduate education, residency training, and continuing medical education, encompassing interdisciplinary curricula, collaborative workshops, with locally adapted multilingual resources. It is suggested that implementation of such a framework should be supported by national professional societies and development of shared-care pathways, with key outcomes monitored. Strengthening cross-disciplinary knowledge, skills, and attitudes in this way may improve TD detection, reduce avoidable progression, and promote coordinated, patient-centred care in selected Asian regions. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature. FAU - Bhidayasiri, Roongroj AU - Bhidayasiri R AUID- ORCID: 0000-0002-6901-2064 AD - Chulalongkorn Centre of Excellence for Parkinson's Disease & Related Disorders, Department of Medicine, Faculty of Medicine, Chulalongkorn University and King Chulalongkorn Memorial Hospital, Thai Red Cross Society, 1873 Rama 4 Road, 10330, Bangkok, Thailand. rbh@chulapd.org. AD - The Academy of Science, The Royal Society of Thailand, Bangkok, 10330, Thailand. rbh@chulapd.org. FAU - Wu, Yun-Cheng AU - Wu YC AD - Department of Neurology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Hongkou District, Shanghai, 200080, China. FAU - Tunvirachaisakul, Chavit AU - Tunvirachaisakul C AD - Centre of Excellence in Cognitive Impairment and Dementia, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. FAU - Kim, Jong-Min AU - Kim JM AD - Department of Neurology, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, South Korea. FAU - Kishi, Taro AU - Kishi T AD - Department of Psychiatry, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan. FAU - Zhang, Tianhong AU - Zhang T AD - Shanghai Mental Health Centre, Neuromodulation Centre, Shanghai Engineering Research Centre of Intelligent Psychological Evaluation and Intervention, Shanghai Jiaotong University School of Medicine, Shanghai, 200030, China. FAU - Hong, Jung Kyung AU - Hong JK AD - Department of Psychiatry, Seoul National University Bundang Hospital, Seongnam, South Korea. FAU - Watanabe, Hirohisa AU - Watanabe H AD - Department of Neurology, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan. LA - eng GR - C01F670185/Thailand Science Research and Innovation/ GR - N42A680591, N35E680087/National Research Council of Thailand/ GR - RA-MF-22/69/Chulalongkorn University/ PT - Journal Article DEP - 20261027 PL - Austria TA - J Neural Transm (Vienna) JT - Journal of neural transmission (Vienna, Austria : 1996) JID - 9702341 SB - IM OTO - NOTNLM OT - Asia OT - Educational initiatives OT - Neurologists OT - Psychiatrists OT - Tardive dyskinesia COIS- Declarations. Conflict of interest: The authors declare no competing interests. Ethical approval: We confirm that all authors have read the Journal's guidelines on compliance with ethical standards and affirm that this work is consistent with those guidelines. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:12 PHST- 2026/09/05 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:12 [entrez] AID - 10.1007/s00702-026-03318-6 [pii] AID - 10.1007/s00702-026-03318-6 [doi] PST - aheadofprint SO - J Neural Transm (Vienna). 2026 Oct 27. doi: 10.1007/s00702-026-03318-6. PMID- 42944843 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1536-5964 (Electronic) IS - 0025-7974 (Print) IS - 0025-7974 (Linking) VI - 105 IP - 43 DP - 2026 Oct 23 TI - Histopathological findings in laparoscopic sleeve gastrectomy specimens and their relation to preoperative endoscopy: A retrospective cross-sectional study of 954 patients. PG - e51246 LID - 10.1097/MD.0000000000051246 [doi] LID - e51246 AB - Laparoscopic sleeve gastrectomy (LSG) is the most frequently performed bariatric surgery. Although it is generally considered safe, a thorough preoperative assessment is essential to minimize the risk of complications. The need for histopathological examination of resected gastric specimens in bariatric surgery remains a subject of debate, particularly regarding the role of preoperative esophagogastroduodenoscopy (EGD). This study aimed to determine the prevalence of histopathological findings in resected specimens following sleeve gastrectomy and to assess their agreement with preoperative EGD findings at a tertiary center. This retrospective cross-sectional study examined patients who underwent LSG between 2009 to 2021. The data included demographics, comorbidities, preoperative EGD findings, and routine postoperative histopathology of the resected specimens. Diagnostic accuracy of preoperative EGD was assessed against resected-specimen histopathology. Among the 954 patients who underwent LSG, 67.1% were female, and 84.7% were Saudi nationals. Abnormal histopathological findings were observed in 45.5% (434/954) of the resected specimens, with gastritis being the predominant finding, present in 98.2% (426/434) of these. Preoperative EGD was performed in a subgroup of 357 patients (37.4%); in these, gastritis was the most prevalent EGD finding (50.1%). Helicobacter pylori was identified in 9.5% of patients who underwent preoperative EGD and in 8.0% of all resected specimens. Less common histopathological findings were metaplasia (0.6%), gastrointestinal stromal tumors (0.3%), gastric polyps (0.2%) and grade 1 gastric carcinoid (0.1%). Among the 357 patients who underwent preoperative EGD, sensitivity was 99.5%, specificity 98.3%, positive predictive value 98.4%, negative predictive value 99.4% and accuracy 98.9% against resected-specimen histopathology. Gastritis is the most prevalent histopathological finding in resected specimens following sleeve gastrectomy and on preoperative EGD, although significant pathologies are infrequent. In the selected subgroup of patients who underwent preoperative EGD, a normal EGD showed high agreement with normal histopathology of the resected specimen; these findings do not support omitting histopathological examination of resected specimens, and prospective multicenter studies are needed. Helicobacter pylori was frequently identified and was strongly associated with gastritis in unadjusted analyses; premalignant findings were too few to assess. CI - Copyright (c) 2026 the Author(s). Published by Wolters Kluwer Health, LLC. FAU - Abulfaraj, Moaz AU - Abulfaraj M AD - Department of Surgery, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia. FAU - Aljiffry, Murad M AU - Aljiffry MM AD - Department of Surgery, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia. FAU - Alhazmi, Mohammed F AU - Alhazmi MF AD - Department of Surgery, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia. FAU - Maghrabi, Ashraf A AU - Maghrabi AA AD - Department of Surgery, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia. FAU - Bokhary, Rana Y AU - Bokhary RY AD - Department of Pathology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia. FAU - Alotaibi, Abdulrahman Faihan A AU - Alotaibi AFA AD - Surgery Department, King Faisal Medical Complex, Taif, Saudi Arabia. FAU - Fouad, Rania A AU - Fouad RA AD - Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, Riyadh, Saudi Arabia. AD - Department of Medical Biochemistry, College of Medicine, Zagazig University, Zagazig, Egypt. FAU - El-Sherbiny, Mohamed AU - El-Sherbiny M AD - Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, Riyadh, Saudi Arabia. AD - Research Center, Deanship of Scientific Research and Post-Graduate Studies, AlMaarefa University, Riyadh, Saudi Arabia. FAU - Ebrahim, Hasnaa Ali AU - Ebrahim HA AD - Department of Basic Medical Sciences, College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia. FAU - Ali, Tagwa Salah Ahmed Mohammed AU - Ali TSAM AD - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, Saudi Arabia. AD - Department of Parasitology and Medical Entomology, Faculty of Medical laboratory Science, Sudan University of Science and Technology, Khartoum, Sudan. FAU - Elsherbini, Dalia Mahmoud Abdelmonem AU - Elsherbini DMA AUID- ORCID: 0000-0001-5262-6134 AD - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, Saudi Arabia. AD - Department of Anatomy, Faculty of Medicine, Mansoura University, Mansoura, Egypt. LA - eng PT - Journal Article PL - United States TA - Medicine (Baltimore) JT - Medicine JID - 2985248R SB - IM MH - Humans MH - Female MH - Cross-Sectional Studies MH - Male MH - Retrospective Studies MH - *Gastrectomy/methods MH - Middle Aged MH - Adult MH - *Endoscopy, Digestive System MH - *Laparoscopy/methods MH - *Bariatric Surgery/methods MH - Gastritis/pathology MH - Preoperative Care MH - Helicobacter Infections MH - Helicobacter pylori/isolation & purification MH - Saudi Arabia MH - Gastrointestinal Stromal Tumors/pathology MH - Stomach Neoplasms/pathology PMC - PMC13706432 OTO - NOTNLM OT - esophagogastroduodenoscopy OT - gastritis OT - helicobacter pylori infection OT - histopathological examination OT - laparoscopic sleeve gastrectomy COIS- The authors have no conflicts of interest to declare. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/23 CRDT- 2026/10/27 12:09 PHST- 2024/05/29 00:00 [received] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:09 [entrez] PHST- 2026/10/23 00:00 [pmc-release] AID - 00005792-202610230-00021 [pii] AID - MD-D-24-05923 [pii] AID - 10.1097/MD.0000000000051246 [doi] PST - ppublish SO - Medicine (Baltimore). 2026 Oct 23;105(43):e51246. doi: 10.1097/MD.0000000000051246. PMID- 42944838 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1365-2982 (Electronic) IS - 1350-1925 (Linking) VI - 38 IP - 10 DP - 2026 Oct TI - Body Surface Gastric Mapping Phenotypes and Treatment Escalation in Chronic Gastroduodenal Symptoms. LID - e70481 [pii] LID - 10.1111/nmo.70481 [doi] AB - BACKGROUND: Chronic gastroduodenal symptoms have overlapping mechanisms, but physiologic features of refractory disease remain unclear. We evaluated associations of body surface gastric mapping (BSGM) phenotypes and gastric emptying with treatment escalation. METHODS: We retrospectively studied adults undergoing BSGM at a tertiary center, excluding prior foregut surgery. Patients were classified as Motor Predominant or Non Motor Predominant using Auckland Classification v1.0. Treatment escalation comprised advanced intervention or nutritional support. KEY RESULTS: Among 146 patients (median age, 35 years; 83.6% female), 55 (37.7%) were Motor Predominant and 55/137 (40.1%) had delayed gastric emptying. Escalation occurred in 47 (32.2%), including 44 before BSGM. Delayed emptying was associated with escalation after adjustment for phenotype, age, and sex (OR 8.09, 95% CI: 3.39-19.33). Within delayed emptying, escalation was more frequent with a Motor Predominant phenotype (77.4% vs. 41.7%; Holm-adjusted p = 0.020), despite similar gastric retention; grouping by any spectral abnormality showed a smaller, nonsignificant difference (p = 0.088). No statistically significant difference was detected with normal emptying. Among Motor Predominant phenotypes, Dysrhythmia had the strongest observed associations with escalation (OR 4.02, 95% CI: 1.41-11.86) and delayed emptying (OR 6.13, 95% CI: 1.97-21.69) versus the Non Motor Predominant group. CONCLUSIONS AND INFERENCES: Auckland phenotyping more clearly identified a phenotype with more refractory disease among patients with delayed gastric emptying than grouping by any spectral abnormality. Dysrhythmia had the strongest observed association among Motor Predominant phenotypes. Because most escalation preceded BSGM, these findings characterize an established refractory course but cannot predict future escalation. BSGM and gastric emptying testing may provide complementary information. CI - (c) 2026 John Wiley & Sons Ltd. FAU - Sirimongkolkasem, Jarongkorn AU - Sirimongkolkasem J AUID- ORCID: 0000-0002-3628-4290 AD - Division of Gastroenterology & Hepatology, Stanford University, Palo Alto, California, USA. AD - Division of Gastroenterology & Hepatology, Department of Medicine, King Chulalongkorn Memorial Hospital, Bangkok, Thailand. AD - Center of Excellence in Neurogastroenterology and Motility, Chulalongkorn University, Bangkok, Thailand. FAU - Kodilinye, Syed Matthew AU - Kodilinye SM AUID- ORCID: 0000-0002-1487-6674 AD - Division of Gastroenterology & Hepatology, Stanford University, Palo Alto, California, USA. AD - Department of Neurology & Neurological Sciences, Stanford University, Palo Alto, California, USA. FAU - Zhou, Wendy AU - Zhou W AUID- ORCID: 0000-0002-1585-9497 AD - Division of Gastroenterology & Hepatology, Stanford University, Palo Alto, California, USA. FAU - Clarke, John O AU - Clarke JO AUID- ORCID: 0000-0003-2625-1169 AD - Division of Gastroenterology & Hepatology, Stanford University, Palo Alto, California, USA. FAU - Neshatian, Leila AU - Neshatian L AUID- ORCID: 0000-0003-4153-5012 AD - Division of Gastroenterology & Hepatology, Stanford University, Palo Alto, California, USA. FAU - Nguyen, Linda AU - Nguyen L AUID- ORCID: 0000-0002-3498-6897 AD - Division of Gastroenterology & Hepatology, Stanford University, Palo Alto, California, USA. AD - Hoag Digestive Health Institute, Newport Beach, California, USA. LA - eng PT - Journal Article PL - England TA - Neurogastroenterol Motil JT - Neurogastroenterology and motility JID - 9432572 SB - IM MH - Humans MH - Female MH - Phenotype MH - Male MH - Retrospective Studies MH - *Gastric Emptying/physiology MH - Adult MH - Middle Aged MH - Chronic Disease MH - *Stomach/physiopathology OAB - Chronic stomach symptoms such as nausea, feeling full after small meals, bloating, and pain can be difficult to treat. Body surface gastric mapping (BSGM) is a non-invasive test that uses a patch on the abdomen to measure the stomach's electrical activity. In 146 patients, we examined how BSGM patterns and stomach emptying related to how often patients received advanced procedures or nutritional support. Slow stomach emptying was most strongly linked to these treatments. Among patients with slow emptying, those with an abnormal "motor" pattern on BSGM had received these treatments more often, despite similar severity of delayed stomach emptying. Dysrhythmia, a disordered electrical rhythm, showed the strongest link among the abnormal motor patterns. Most patients had received these treatments before BSGM was performed. The study therefore cannot show whether BSGM predicts future treatment needs or helps physicians choose treatments. However, when interpreted alongside stomach emptying tests, BSGM may help clinicians better understand patients with difficult-to-treat symptoms. Future studies that perform BSGM before advanced treatment are needed to show whether it can help guide treatment decisions and improve outcomes. OABL- eng OTO - NOTNLM OT - Auckland classification OT - body surface gastric mapping OT - gastric dysrhythmia OT - gastric emptying OT - gastroparesis OT - treatment escalation EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:08 PHST- 2026/09/24 00:00 [revised] PHST- 2026/07/09 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:08 [entrez] AID - e70481 [pii] AID - 10.1111/nmo.70481 [doi] PST - ppublish SO - Neurogastroenterol Motil. 2026 Oct;38(10):e70481. doi: 10.1111/nmo.70481. PMID- 42944827 OWN - NLM STAT- Publisher LR - 20261027 IS - 1920-7476 (Electronic) IS - 0008-4263 (Linking) DP - 2026 Oct 27 TI - Post-breast cancer diagnosis screening mammography among First Nations and non-First Nations women in Ontario : A retrospective cohort study. LID - 10.17269/s41997-026-01272-0 [doi] AB - OBJECTIVES: First Nations women in Canada have historically experienced lower breast cancer screening participation and worse outcomes than non-First Nations women. While most research has focused on pre-diagnosis screening, less is known about follow-up care. This study examined post-diagnosis mammography use among First Nations and non-First Nations women in Ontario and identified factors associated with follow-up screening among First Nations women. METHODS: This retrospective cohort study included 190 First Nations and 506 non-First Nations women diagnosed with invasive breast cancer between 1995 and 2004, with follow-up screening data available through 2019. Descriptive statistics compared demographic, clinical, and treatment characteristics. Logistic regression models were used among First Nations women to identify factors associated with follow-up screening. RESULTS: No statistically significant differences were observed in the unadjusted comparisons of post-treatment screening rates, frequency, timing, and intervals between First Nations and non-First Nations women. Among First Nations women, older age (OR = 0.42; 95% CI, 0.19-0.94), rural residence (OR = 0.35; 95% CI, 0.17-0.73), and lower income (OR = 0.20; 95% CI, 0.06-0.69) were among the factors associated with reduced screening. Radiotherapy (OR = 7.48; 95% CI, 3.38-16.56) and less severe surgery (OR = 7.19; 95% CI, 3.31-15.63) were associated with higher odds of screening. CONCLUSION: While screening rates among First Nations women are improving, disparities persist. Strengthening continuity of care, primary care access, and culturally safe survivorship support is essential to achieving equity. CI - (c) 2026. The Author(s). FAU - Saini, Garima AU - Saini G AUID- ORCID: 0009-0006-8641-9540 AD - Indigenous Health Unit, Ontario Health, Toronto, ON, Canada. garima.saini@ontariohealth.ca. FAU - Ellison, Lisa K AU - Ellison LK AUID- ORCID: 0009-0005-4065-1216 AD - Indigenous Health Unit, Ontario Health, Toronto, ON, Canada. FAU - Chiarelli, Anna M AU - Chiarelli AM AUID- ORCID: 0000-0002-7382-513X AD - Dalla Lana School of Public Health, University of Toronto, Toronto, ON, Canada. FAU - Kewayosh, Alethea AU - Kewayosh A AD - Indigenous Health Unit, Ontario Health, Toronto, ON, Canada. FAU - Sheppard, Amanda J AU - Sheppard AJ AUID- ORCID: 0000-0001-5240-2704 AD - Indigenous Health Unit, Ontario Health, Toronto, ON, Canada. LA - eng PT - Journal Article DEP - 20261027 PL - Switzerland TA - Can J Public Health JT - Canadian journal of public health = Revue canadienne de sante publique JID - 0372714 SB - IM OTO - NOTNLM OT - Breast OT - Breast neoplasms OT - Female OT - First Nations OT - Follow-up studies OT - Mammography COIS- Declarations. Ethics approval: This study received ethical approval from the University of Toronto Research Ethics Board (REB) and is supported by the Joint Ontario Indigenous Health Committee (JOIHC), an advisory group comprised of representatives from Political Territorial Organizations and provincial First Nations, Inuit, Metis, and urban Indigenous organizations. Consent to participate: Not applicable. Consent for publication: Not applicable. Conflict of interest: The authors declare no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:08 PHST- 2025/12/04 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:08 [entrez] AID - 10.17269/s41997-026-01272-0 [pii] AID - 10.17269/s41997-026-01272-0 [doi] PST - aheadofprint SO - Can J Public Health. 2026 Oct 27. doi: 10.17269/s41997-026-01272-0. PMID- 42944826 OWN - NLM STAT- Publisher LR - 20261027 IS - 1880-4233 (Electronic) IS - 1340-6868 (Linking) DP - 2026 Oct 27 TI - Prognostic performance of the Japanese Breast Cancer Society pathological response classification after neoadjuvant chemotherapy. LID - 10.1007/s12282-026-01933-5 [doi] AB - BACKGROUND: Pathological response after neoadjuvant chemotherapy is strongly associated with prognosis in breast cancer. However, further prognostic stratification is needed among patients with residual invasive disease because their outcomes vary widely. The six-tier Japanese Breast Cancer Society (JBCS) pathological treatment effect classification provides a graded assessment of histological response in the primary breast tumor, but its prognostic validity has not been sufficiently established in a large cohort. METHODS: We retrospectively analyzed 1,003 patients with invasive breast cancer who underwent surgery after neoadjuvant chemotherapy at two institutions between 2006 and 2022. Distant recurrence-free survival (DRFS) was evaluated according to the six-tier JBCS classification. Multivariable Cox models incorporating either the six-tier JBCS classification or ypT were adjusted for age, breast cancer subtype, and ypN and compared using the corrected Akaike information criterion (AICc) and Bayesian information criterion (BIC). Exploratory analyses also evaluated simplified classifications. RESULTS: The six-tier JBCS classification significantly stratified DRFS (log-rank P < 0.001). Five-year DRFS increased from 60.6% for Grade 0 to 94.4% for Grade 3, although several intermediate categories overlapped. The classification remained independently associated with DRFS (global P < 0.001). However, the ypT model showed better fit (AICc, 3052.00 vs. 3065.71; BIC, 3105.70 vs. 3124.28). Exploratory simplification analyses retained significant prognostic stratification, although none showed better model performance than ypT. CONCLUSIONS: The six-tier JBCS classification provides clinically meaningful prognostic information after neoadjuvant chemotherapy. However, ypT showed better model fit, and overlap among intermediate categories suggests scope for refinement. CI - (c) 2026. The Author(s), under exclusive licence to The Japanese Breast Cancer Society. FAU - Ushiyama, Yumiko AU - Ushiyama Y AD - Department of Breast Oncology, Faculty of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. FAU - Ueki, Yuko AU - Ueki Y AD - Department of Breast Oncology, Faculty of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. FAU - Horimoto, Yoshiya AU - Horimoto Y AUID- ORCID: 0000-0001-8935-0768 AD - Department of Breast Oncology, Faculty of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. horimoto@juntendo.ac.jp. AD - Department of Human Pathology, Faculty of Medicine, Juntendo University, Tokyo, Japan. horimoto@juntendo.ac.jp. AD - Department of Breast Surgery and Oncology, Tokyo Medical University, Tokyo, Japan. horimoto@juntendo.ac.jp. FAU - Yoshino, Mariko AU - Yoshino M AD - Department of Breast Oncology, Faculty of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. FAU - Ishizuka, Yumiko AU - Ishizuka Y AUID- ORCID: 0000-0002-1588-3067 AD - Department of Breast Oncology, Faculty of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. FAU - Semba, Ryoko AU - Semba R AUID- ORCID: 0009-0007-8670-4357 AD - Department of Breast Oncology, Faculty of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. FAU - Shimizu, Hideo AU - Shimizu H AD - Department of General Surgery, Juntendo University Nerima Hospital, Tokyo, Japan. FAU - Ogura, Kanako AU - Ogura K AD - Department of Diagnostic Pathology, Juntendo University Nerima Hospital, Tokyo, Japan. FAU - Hayashi, Takuo AU - Hayashi T AUID- ORCID: 0000-0002-8544-9370 AD - Department of Human Pathology, Faculty of Medicine, Juntendo University, Tokyo, Japan. FAU - Ishikawa, Takashi AU - Ishikawa T AD - Department of Breast Surgery and Oncology, Tokyo Medical University, Tokyo, Japan. FAU - Watanabe, Junichiro AU - Watanabe J AUID- ORCID: 0000-0001-9226-895X AD - Department of Breast Oncology, Faculty of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. FAU - Kutomi, Goro AU - Kutomi G AD - Department of Breast Oncology, Faculty of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. LA - eng PT - Journal Article DEP - 20261027 PL - Japan TA - Breast Cancer JT - Breast cancer (Tokyo, Japan) JID - 100888201 SB - IM OTO - NOTNLM OT - Breast cancer OT - Neoadjuvant chemotherapy OT - Pathological response OT - Prognosis OT - Residual disease COIS- Declarations. Conflict of interest: Yoshiya Horimoto serves as Editorial Board member of Breast Cancer. To minimize bias, he was excluded from all editorial decision-making related to the acceptance of this article for publication. The remaining authors declare no conflicts of interest. Ethical approval: This study was approved by the institutional review boards of the participating hospitals (approval H19-0289). Informed consent: Not applicable. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:07 PHST- 2026/08/11 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:07 [entrez] AID - 10.1007/s12282-026-01933-5 [pii] AID - 10.1007/s12282-026-01933-5 [doi] PST - aheadofprint SO - Breast Cancer. 2026 Oct 27. doi: 10.1007/s12282-026-01933-5. PMID- 42944818 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1573-2983 (Electronic) IS - 0269-4042 (Print) IS - 0269-4042 (Linking) VI - 48 IP - 16 DP - 2026 Oct 27 TI - Portable X-ray fluorescence (pXRF) analysis of heavy metals in contaminated soils: a case study from Varta Insel, Hagen. LID - 10.1007/s10653-026-03538-5 [doi] LID - 667 AB - Urban soils contaminated with persistent and toxic heavy metals are found worldwide. Their remediation is essential to prevent harm to humans and the environment. The integration of portable X-ray fluorescence spectroscopy (pXRF) as a field analytical instrument in remediation projects offers the possibility to rapidly assess soils at low costs. In this study, we test the potential of pXRF in contaminated soil assessment at a former battery factory "Varta Insel" in Hagen, Germany. Soil samples were analyzed (1) by pXRF on site, (2) by pXRF under laboratory conditions, and (3) by microwave assisted aqua regia digestion with inductively coupled plasma optical emission spectroscopy (ICP-OES). Direct on-site measurements were useful for supporting field decisions and selecting samples for further analysis, but results were strongly influenced by matrix effects, grain size, and moisture content, limiting their reliability. Dried and sieved samples were measured by pXRF under laboratory conditions through low-density polyethylene sample bags. The laboratory pXRF results agreed with the more expensive ICP-OES analysis in 94% of cases when classifying samples above or below German regulatory limits. However, pXRF systematically underestimated lead concentrations and overestimated arsenic and chromium. Hence, we conclude that pXRF is not a stand-alone solution for heavy metal analysis of contaminated soil samples. However, it can effectively guide the selection of samples requiring detailed ICP-OES analysis and contribute to higher-density data collection, ultimately enhancing spatial resolution. CI - (c) 2026. The Author(s). FAU - Kaulingfrecks, Julius AU - Kaulingfrecks J AD - Institute of Geology and Paleontology, University of Munster, Correnstrasse 24, 48149, Munster, Germany. AD - Arcadis Germany B.V. & Co. KG, Europaplatz 3, 64293, Darmstadt, Germany. FAU - De Vleeschouwer, David AU - De Vleeschouwer D AD - Institute of Geology and Paleontology, University of Munster, Correnstrasse 24, 48149, Munster, Germany. FAU - Michalski, Anja AU - Michalski A AD - Arcadis Germany B.V. & Co. KG, Europaplatz 3, 64293, Darmstadt, Germany. FAU - Achten, Christine AU - Achten C AD - Institute of Geology and Paleontology, University of Munster, Correnstrasse 24, 48149, Munster, Germany. achten@uni-muenster.de. LA - eng PT - Journal Article DEP - 20261027 PL - Netherlands TA - Environ Geochem Health JT - Environmental geochemistry and health JID - 8903118 RN - 0 (Soil Pollutants) RN - 0 (Metals, Heavy) RN - N712M78A8G (Arsenic) RN - 0 (Soil) SB - IM MH - *Soil Pollutants/analysis MH - *Spectrometry, X-Ray Emission/methods MH - *Metals, Heavy/analysis MH - *Environmental Monitoring/methods/instrumentation MH - Germany MH - Arsenic/analysis MH - Soil/chemistry PMC - PMC13714078 OTO - NOTNLM OT - Heavy metals OT - On site analysis OT - Remediation OT - Urban soil OT - pXRF COIS- Declarations. Conflict of interest: The authors have not disclosed any competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 12:07 PHST- 2026/05/05 00:00 [received] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:07 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 10.1007/s10653-026-03538-5 [pii] AID - 3538 [pii] AID - 10.1007/s10653-026-03538-5 [doi] PST - epublish SO - Environ Geochem Health. 2026 Oct 27;48(16):667. doi: 10.1007/s10653-026-03538-5. PMID- 42944811 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1436-5073 (Electronic) IS - 0026-3672 (Linking) VI - 193 IP - 11 DP - 2026 Oct 27 TI - Sequential fluorescence modulation in an aptamer-functionalized bimetallic Ni/Fe-MOF for smartphone-assisted ratiometric detection of Staphylococcus aureus. LID - 761 [pii] LID - 10.1007/s00604-026-08496-4 [doi] AB - Fluorescence modulation through enhancement and spectral tuning is important for improving the analytical performance of luminescence-based sensing platforms. In this study, a label-free fluorescent aptasensor was developed by functionalizing a bimetallic nickel-iron metal-organic framework (Ni/Fe-MOF) with a specific single-stranded DNA aptamer for the detection of Staphylococcus aureus (S. aureus). The measured quantum yields of the monometallic Fe-MOF and Ni-MOF were 15% and 11%, respectively, whereas the Ni/Fe-MOF exhibited a quantum yield of 26%. Following the aptamer functionalization step, the quantum yield increased to 31%, indicating a change in the fluorescence properties of the sensing material. Upon exposure to S. aureus, further modulation of the fluorescence response was observed, including an increased contribution of the green emission component around 520 nm relative to the blue emission around 420 nm. This sequential fluorescence modulation enabled ratiometric detection of S. aureus, with limits of detection of 1.1 CFU.mL(-)(1) using conventional fluorometric measurements and 1.33 CFU.mL(-)(1) using smartphone-assisted analysis. The sensing platform demonstrated selectivity and rapid response under the optimized experimental conditions. In spiked wastewater samples, recoveries ranged from 94.5% to 97.9%, with RSD values below 0.63%. These results demonstrate the potential of the developed aptasensor as a portable platform for bacterial detection in aqueous and wastewater samples, while its application to more complex matrices, including food samples and biofilm-associated bacteria, will require appropriate sample pretreatment and further experimental validation. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature. FAU - Fatah, Shilan Arif AU - Fatah SA AD - Department of Pharmacy, Kurdistan Technical Institute, Sulaimani, KGR, 46001, Iraq. FAU - Ameen, Sameera Sh Mohammed AU - Ameen SSM AD - Department of Chemistry, College of Science, University of Zakho, Zakho, Kurdistan Region, 42002, Iraq. FAU - Hassan, Payam B AU - Hassan PB AD - Department of Chemistry, College of Science University of Sulaimani, Sulaymaniyah, Kurdistan Region, 46002, Iraq. FAU - Raza, Rzgar Sirwan AU - Raza RS AD - Department of Artificial Intelligence and Data Science, College of Science and Technology, University of Human Development, Sulaymaniyah, Kurdistan Region, Iraq. FAU - Omer, Khalid M AU - Omer KM AD - Department of Chemistry, College of Science University of Sulaimani, Sulaymaniyah, Kurdistan Region, 46002, Iraq. khalid.omer@univsul.edu.iq. LA - eng PT - Journal Article DEP - 20261027 PL - Austria TA - Mikrochim Acta JT - Mikrochimica acta JID - 7808782 RN - 0 (Aptamers, Nucleotide) RN - 7OV03QG267 (Nickel) RN - E1UOL152H7 (Iron) RN - 0 (Metal-Organic Frameworks) SB - IM MH - *Staphylococcus aureus/isolation & purification/chemistry MH - *Aptamers, Nucleotide/chemistry MH - Nickel/chemistry MH - Iron/chemistry MH - *Biosensing Techniques/methods/instrumentation MH - *Smartphone MH - *Metal-Organic Frameworks/chemistry MH - Limit of Detection MH - Spectrometry, Fluorescence/methods MH - Fluorescence OTO - NOTNLM OT - Staphylococcus aureus OT - Aptamer OT - Biosensor OT - Fluorescence modulation OT - Ni/Fe- metal-organic framework (Ni/Fe-MOF) COIS- Declarations. Competing interests: The authors declare no competing interests. Use of AI: ChatGPT (OpenAI) was used partially during the preparation of this manuscript to assist with language editing, grammar correction, and improvement of clarity and readability. In addition, AI tools were used partially in the preparation and enhancement of some graphical elements and schemes. The AI tools were not used to generate or alter the underlying scientific data or results. All AI-assisted content was reviewed and verified by the authors, who remain fully responsible for the accuracy and integrity of the manuscript and its figures. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:07 PHST- 2026/08/29 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:07 [entrez] AID - 10.1007/s00604-026-08496-4 [pii] AID - 10.1007/s00604-026-08496-4 [doi] PST - epublish SO - Mikrochim Acta. 2026 Oct 27;193(11):761. doi: 10.1007/s00604-026-08496-4. PMID- 42944807 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1436-5073 (Electronic) IS - 0026-3672 (Linking) VI - 193 IP - 11 DP - 2026 Oct 27 TI - Molecular recognition elements-integrated multimodal nanosensing of aflatoxin B1: design principles and self-calibrating paradigms. LID - 764 [pii] LID - 10.1007/s00604-026-08473-x [doi] AB - Aflatoxin B1 (AFB1) is a potent hepatocarcinogenic mycotoxin that poses severe health risks through contaminated agricultural products. Rapid, sensitive, reliable, and field-deployable detection methods are therefore critical for food safety. Conventional instrumental techniques suffer from high cost, complex operation, and unsuitability for on-site screening. Biosensors employing molecular recognition elements (MREs) such as antibodies, aptamers, and molecularly imprinted polymers (MIPs) have emerged as promising alternatives, offering enhanced specificity and sensitivity. Nevertheless, single-mode sensing platforms remain fundamentally vulnerable to environmental interferences, matrix effects, and instrumental drift, leading to compromised accuracy and reliability. In this context, it is essential to conduct an in-depth review of AFB1 detection strategies to advance the establishment of a multimodal nanosensing paradigm. It details the integration of functional nanomaterials, including metal nanoparticles, metal oxides, carbon-based nanomaterials, metal-organic frameworks and their derivatives, and MXenes, with MREs to construct dual-mode and triple-mode sensing platforms. The molecular specificity conferred by MREs ensures selective target capture in complex food matrices. The multimodal signal transduction, encompassing fluorescent-colorimetric (FL-CL), surface-enhanced Raman scattering-fluorescent (SERS-FL), electrochemical-optical/photoelectrochemical (EC-Optical/PEC), and triple-mode configurations, provides the built-in cross-validation and self-calibration capabilities of detection results. Finally, the challenges and future prospects are discussed, including further integration and coordination of multimodal signals, miniaturized portable sensing platforms, advanced nanomaterials, and detection-remediation integration. This review advances the rational design of next-generation AFB1 multimodal self-calibrating nanosensors with integrated MREs, and promotes their practical deployment in food safety regulation, and public health protection. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature. FAU - Li, Fang AU - Li F AD - School of Information Engineering, Tianjin University of Commerce, Tianjin, China. lifang_6952@163.com. FAU - Xu, Longping AU - Xu L AD - Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, No. 409, Guangrong Road, Tianjin, China. FAU - Qiao, Jingwen AU - Qiao J AD - School of Information Engineering, Tianjin University of Commerce, Tianjin, China. FAU - Zhao, Mengni AU - Zhao M AD - Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, No. 409, Guangrong Road, Tianjin, China. FAU - Liu, Ruowei AU - Liu R AD - Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, No. 409, Guangrong Road, Tianjin, China. FAU - Zhang, Jiayun AU - Zhang J AD - Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, No. 409, Guangrong Road, Tianjin, China. FAU - Wang, Mengwei AU - Wang M AD - Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, No. 409, Guangrong Road, Tianjin, China. FAU - Meng, Dan AU - Meng D AD - Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, No. 409, Guangrong Road, Tianjin, China. mengd@tjcu.edu.cn. FAU - Wu, Zijian AU - Wu Z AUID- ORCID: 0000-0001-7093-3023 AD - Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, No. 409, Guangrong Road, Tianjin, China. wzjian@tjcu.edu.cn. AD - Key Lab of Agricultural Products Low Carbon Cold Chain, Ministry of Agriculture and Rural Affairs, Tianjin, China. wzjian@tjcu.edu.cn. AD - Tianjin Key Laboratory of Edible Probiotics, Tianjin, China. wzjian@tjcu.edu.cn. LA - eng GR - GHYF2025021/International Science & Technology Cooperation Program of Hainan Province/ PT - Journal Article PT - Review DEP - 20261027 PL - Austria TA - Mikrochim Acta JT - Mikrochimica acta JID - 7808782 RN - 9N2N2Y55MH (Aflatoxin B1) RN - 0 (Aptamers, Nucleotide) SB - IM MH - *Aflatoxin B1/analysis MH - *Biosensing Techniques/methods MH - Aptamers, Nucleotide/chemistry MH - Food Contamination/analysis MH - Nanostructures/chemistry MH - Metal Nanoparticles/chemistry MH - Electrochemical Techniques/methods MH - Colorimetry/methods OTO - NOTNLM OT - Aflatoxin B1 OT - Food safety OT - Molecular recognition elements OT - Multimodal nanosensing OT - Self-calibration COIS- Declarations. Competing interests: The authors declare no competing interests. AI use statement: No artificial intelligence (AI) tools were used in the preparation of this manuscript, including for text, figures, and graphical abstract. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:07 PHST- 2026/07/14 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:07 [entrez] AID - 10.1007/s00604-026-08473-x [pii] AID - 10.1007/s00604-026-08473-x [doi] PST - epublish SO - Mikrochim Acta. 2026 Oct 27;193(11):764. doi: 10.1007/s00604-026-08473-x. PMID- 42944804 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1432-1068 (Electronic) IS - 1633-8065 (Linking) VI - 36 IP - 1 DP - 2026 Oct 27 TI - Closed percutaneous reduction versus mini-open sinus tarsi approach for fixation of displaced intra-articular calcaneal fractures: a systematic review and meta-analysis. LID - 423 [pii] LID - 10.1007/s00590-026-04992-8 [doi] AB - BACKGROUND: Superiority of minimally invasive surgical approaches for displaced intra-articular calcaneal fractures (DIACF) remains debated. This review aimed to compare surgical outcomes between two approaches: closed percutaneous reduction (PR) and mini-open sinus tarsi approach (STA). METHODS: A systematic search identified studies comparing PR vs. STA for acute traumatic DIACFs. Complication rates, operative parameters, functional and radiological outcomes were extracted and analysed. RESULTS: 837 patients were included from eight eligible studies, with Sanders type II and III fractures. PR demonstrated a 53% risk reduction in overall complication rate vs. STA (RR 0.47, 95% CI [0.27-0.83], p = 0.009), including wound complications. AOFAS scores marginally favoured PR vs. STA (MD 0.82 points, p = 0.034), with this difference having doubtful clinical relevance. Radiological parameters were comparable, although calcaneal width remained wider with PR. PR was also associated with shorter times to surgery, length of stays and intra-operative times, although with high heterogeneity in the pooled outcome estimates. CONCLUSION: PR demonstrates lower complication rates with comparable functional and radiological outcomes vs. STA in fixation of less complex DIACFs, and may be an acceptable fixation strategy for these fracture patterns over STA. LEVEL OF EVIDENCE: Level III. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag France SAS, part of Springer Nature. FAU - de Sa, Russell AU - de Sa R AUID- ORCID: 0009-0000-5624-3456 AD - Department of Trauma and Orthopaedics, Imperial College Healthcare NHS Trust, London, UK. russell.desa@nhs.net. FAU - Durand-Hill, Matthieu AU - Durand-Hill M AD - Department of Trauma and Orthopaedics, Whittington Health NHS Trust, London, UK. FAU - Sivanadarajah, Naveethan AU - Sivanadarajah N AD - Department of Trauma and Orthopaedics, Imperial College Healthcare NHS Trust, London, UK. FAU - Kwok, Iris AU - Kwok I AD - Department of Trauma and Orthopaedics, Imperial College Healthcare NHS Trust, London, UK. FAU - Mushtaq, Nadeem AU - Mushtaq N AD - Department of Trauma and Orthopaedics, Imperial College Healthcare NHS Trust, London, UK. LA - eng PT - Comparative Study PT - Journal Article PT - Meta-Analysis PT - Review PT - Systematic Review DEP - 20261027 PL - France TA - Eur J Orthop Surg Traumatol JT - European journal of orthopaedic surgery & traumatology : orthopedie traumatologie JID - 9518037 SB - IM MH - Humans MH - *Calcaneus/injuries/surgery/diagnostic imaging MH - *Intra-Articular Fractures/surgery/diagnostic imaging MH - *Fracture Fixation, Internal/methods/adverse effects MH - Postoperative Complications/etiology MH - *Closed Fracture Reduction/methods/adverse effects MH - Minimally Invasive Surgical Procedures/methods/adverse effects MH - *Open Fracture Reduction/methods/adverse effects MH - Treatment Outcome MH - Operative Time MH - *Fractures, Bone/surgery MH - Length of Stay OTO - NOTNLM OT - Calcaneal fractures OT - Complications OT - Minimally invasive OT - Percutaneous OT - Sinus tarsi approach COIS- Declarations. Conflict of interest: The authors declare no conflict of interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:06 PHST- 2026/07/28 00:00 [received] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:06 [entrez] AID - 10.1007/s00590-026-04992-8 [pii] AID - 10.1007/s00590-026-04992-8 [doi] PST - epublish SO - Eur J Orthop Surg Traumatol. 2026 Oct 27;36(1):423. doi: 10.1007/s00590-026-04992-8. PMID- 42944798 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1573-4978 (Electronic) IS - 0301-4851 (Linking) VI - 53 IP - 1 DP - 2026 Oct 27 TI - Genome-wide association study identifies genomic regions associated with milk composition and fatty acid traits in Indian Holstein Friesian crossbred cattle. LID - 1835 [pii] LID - 10.1007/s11033-026-13071-4 [doi] AB - BACKGROUND: Crossbred cattle play a pivotal role in India's milk production. This study aimed to identify genomic regions and candidate genes associated with milk yield, milk composition, fatty acid (FA) profile, and desaturase indices in Holstein Friesian crossbred cattle (HFCB). METHODS AND RESULTS: A total of 128 HFCB animals were genotyped using 53,218 SNPs, of which 44,128 passed quality control for Genome-wide association study (GWAS) analysis. Phenotypic evaluation showed moderate variation in test-day milk yield (TDMY) and milk composition traits, while FA profiling revealed predominance of long-chain FAs, particularly palmitic and oleic acids. GWAS identified 36 SNPs across multiple chromosomes, one statistically significant and 35 suggestive. A pleiotropic SNP (ARS-BFGL-NGS-96359) on the Bos Taurus autosome (BTA) 24 was associated with lactose, protein, and solids-not-fat percentages. Several SNPs were associated with short-, medium-, and long-chain saturated fatty acids, with positive and negative allelic effects. Notable effect sizes were observed for oleic acid (C18:1cis) and desaturase indices, particularly DI C17. Positional candidate gene analysis identified HACL1, SGPP2, IMMP2L, MYO5B, ZNF536, GADD45A, PRDM9, and PROKR2, which are involved in lipid metabolism and cellular regulation. Individual SNPs explained 0.087-0.194 of the phenotypic variance, with several loci contributing substantially to milk composition traits and FA C4, C10, and C15. CONCLUSIONS: This study highlights the polygenic nature of milk production and quality traits in HFCB cattle. The study also provides preliminary insights into the genetic architecture of milk quality traits and may contribute to future genomic selection efforts following validation in larger and independent populations. CI - (c) 2026. The Author(s), under exclusive licence to Springer Nature B.V. FAU - Yadav, Ashish AU - Yadav A AUID- ORCID: 0009-0009-6538-685X AD - ICAR - National Dairy Research Institute, Karnal, 132001, Haryana, India. FAU - Singh, Richa AU - Singh R AD - ICAR - National Dairy Research Institute, Karnal, 132001, Haryana, India. FAU - Khan, Kashif Dawood AU - Khan KD AD - ICAR - National Dairy Research Institute, Karnal, 132001, Haryana, India. FAU - Alex, Rani AU - Alex R AD - ICAR - National Dairy Research Institute, Karnal, 132001, Haryana, India. FAU - Gowane, G R AU - Gowane GR AUID- ORCID: 0000-0001-6535-7818 AD - ICAR - National Dairy Research Institute, Karnal, 132001, Haryana, India. gopalgowane@gmail.com. LA - eng GR - 52/IAEC/24/28, dated 21 May 2024/Haryana State Council for Science and Technology/ PT - Journal Article DEP - 20261027 PL - Netherlands TA - Mol Biol Rep JT - Molecular biology reports JID - 0403234 RN - 0 (Fatty Acids) SB - IM MH - Animals MH - Cattle/genetics MH - *Milk/chemistry/metabolism MH - *Fatty Acids/genetics/analysis/metabolism MH - Genome-Wide Association Study/methods MH - Polymorphism, Single Nucleotide/genetics MH - India MH - Female MH - Phenotype MH - Quantitative Trait Loci/genetics MH - Genotype MH - Lactation/genetics OTO - NOTNLM OT - GWAS OT - Holstein Friesian crossbred cattle OT - Karan Fries OT - Milk composition OT - Milk fatty acids COIS- Declarations. Competing interests: The authors have no relevant financial or non-financial interests to disclose. Ethical approval: The collection of blood samples required for genotyping in this study was approved by the Institutional Animal Ethics Committee (IAEC) of ICAR-NDRI under proposal number 52/IAEC/24/28, dated 21 May 2024. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:06 PHST- 2026/03/28 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:06 [entrez] AID - 10.1007/s11033-026-13071-4 [pii] AID - 10.1007/s11033-026-13071-4 [doi] PST - epublish SO - Mol Biol Rep. 2026 Oct 27;53(1):1835. doi: 10.1007/s11033-026-13071-4. PMID- 42944795 OWN - NLM STAT- Publisher LR - 20261027 IS - 1614-7499 (Electronic) IS - 0944-1344 (Linking) DP - 2026 Oct 27 TI - Nitrogen-doped activated carbon from palmyrah kernel shell biomass for efficient CO(2) capture. LID - 10.1007/s11356-026-38332-4 [doi] AB - Anthropogenic carbon dioxide (CO(2)) emissions, primarily from fossil fuel combustion and industrial growth, have significantly contributed to global warming and climate change. This study reports the development of nitrogen-doped activated carbon material, derived from palmyrah kernel shell (PKS), as an adsorbent for CO(2) capture. It was synthesized in a two-step process: melamine as a nitrogen source at varying melamine-to-biomass ratios, followed by chemical activation with KOH. The prepared materials were then characterized by CHNS, FTIR, BET, XPS, SEM-EDX, and TGA to investigate their elemental composition, functional groups, porosity, chemical states, surface morphology, elemental distribution, and thermal stability. The adsorption-desorption behavior was analyzed using a thermo-gravimetric weighing method, while volumetric measurements were performed to further evaluate the pressure-dependent CO(2) capture performance. The nitrogen doping is effected by varying the ratio of PKS biomass to melamine. XPS N1s deconvolution confirmed the presence of pyridinic, pyrrolic, and graphitic nitrogen configurations, while SEM-EDX mapping showed their relatively uniform elemental distribution throughout the carbon matrix. The material with a 3:1 ratio exhibited excellent CO(2) adsorption performance of 174.3 mgg-1 at 25 degrees C and 242.9 mgg-1 at 0 degrees C under ambient pressure, with corresponding desorption of 174.6 mgg-1 at 25 degrees C and 242.6 mgg-1 at 0 degrees C, respectively. Adsorption kinetics were evaluated using the pseudo-second-order model, and thermodynamic parameters were determined to assess the temperature-dependent adsorption. Results showed that the 3:1 N-doped activated carbon derived from PKS exhibited the highest CO(2) uptake and strong CO(2)/N(2) selectivity. Further, the adsorption-desorption cycles establish material stability beyond 20 cycles. Our findings reveal that the lower the nitrogen content, the more efficient the CO(2) capture. The carbonaceous material thus derived from waste biomass enables carbon-based carbon capture, further reducing the carbon footprint of the capture process. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Ilamurugan, Vasanthmani AU - Ilamurugan V AD - Department of Green Energy Technology, Pondicherry University, Kalapet, Puducherry-605014, India. FAU - Baquir Mohammed, Jaffar Ali AU - Baquir Mohammed JA AUID- ORCID: 0000-0003-2363-2513 AD - Department of Green Energy Technology, Pondicherry University, Kalapet, Puducherry-605014, India. jaffarali.get@pondiuni.ac.in. LA - eng PT - Journal Article DEP - 20261027 PL - Germany TA - Environ Sci Pollut Res Int JT - Environmental science and pollution research international JID - 9441769 SB - IM OTO - NOTNLM OT - Activated carbon OT - CO2 adsorption OT - Carbon sequestration OT - Climate change OT - Waste biomass COIS- Declarations. Ethical approval: Not applicable. Consent to participate: Not applicable. Consent to publish: Consent for publication has been duly obtained from the co-author of this manuscript. Competing interests: The authors declare that they have no competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:06 PHST- 2026/06/11 00:00 [received] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:06 [entrez] AID - 10.1007/s11356-026-38332-4 [pii] AID - 10.1007/s11356-026-38332-4 [doi] PST - aheadofprint SO - Environ Sci Pollut Res Int. 2026 Oct 27. doi: 10.1007/s11356-026-38332-4. PMID- 42944791 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 1935-973X (Print) IS - 1935-9748 (Electronic) IS - 1935-9748 (Linking) VI - 19 IP - 1 DP - 2026 Oct 27 TI - Minimally Invasive Surgery for Adult Scoliosis: Recent Updates and Advancements. LID - 10.1007/s12178-026-10064-5 [doi] LID - 71 AB - PURPOSE OF REVIEW: Circumferential minimally invasive surgery (cMIS) for adult scoliosis has evolved over the past five years. Here, we review how advances in patient selection, operative technique, and technology have transformed the capabilities of cMIS for deformity. RECENT FINDINGS: Early cMIS was constrained by a "corrective ceiling" that limited its use to flexible deformities. Patient selection has since grown more nuanced, incorporating surgical complexity, patient-specific factors, and the deformity itself rather than radiographic thresholds alone. Technique has changed in parallel, as correction is now distributed across multiple interbody levels with segmental release, performed through both anterior and lateral techniques. Workflow and technology have adapted as well: single-position prone transpsoas and lateral prepsoas approaches may reduce intraoperative repositioning, staging extends treatment to patients with greater deformity or frailty, and robotics and patient-specific implants improve the precision of planning and execution. These advances have been accompanied by data demonstrating improved patient-reported outcomes and spinopelvic alignment at lower morbidity in appropriately selected patients. With the safety and efficacy of cMIS established, the field continues to push its boundaries toward more challenging pathology, as illustrated by contemporary cases of severe multiplanar deformity and complex revision. The boundaries of cMIS have expanded considerably beyond the flexible, moderate deformities for which these techniques were initially developed. Careful patient selection remains essential, as not all deformities are amenable to minimally invasive correction. Nevertheless, advances in surgical technique, operative workflow, and patient-specific planning have enabled increasingly severe and complex deformities to be addressed while preserving the fundamental advantages of minimally invasive surgery. CI - (c) 2026. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature. FAU - Khan, Farhan AU - Khan F AD - Department of Neurological Surgery, Columbia University Vagelos College of Physicians and Surgeons, New York, NY, USA. fak2121@cumc.columbia.edu. AD - Department of Neurological Surgery, Columbia University Irving Medical Center Neurological Institute, 710 West 168th Street, New York, NY, 10032, USA. fak2121@cumc.columbia.edu. FAU - Hoofnagle, Max D AU - Hoofnagle MD AD - Department of Neurological Surgery, Columbia University Vagelos College of Physicians and Surgeons, New York, NY, USA. FAU - Mummaneni, Praveen V AU - Mummaneni PV AD - Department of Neurological Surgery, University of California San Francisco, San Francisco, CA, USA. FAU - Chan, Andrew K AU - Chan AK AD - Department of Neurological Surgery, Columbia University Vagelos College of Physicians and Surgeons, New York, NY, USA. FAU - Chou, Dean AU - Chou D AD - Department of Neurological Surgery, Columbia University Vagelos College of Physicians and Surgeons, New York, NY, USA. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - United States TA - Curr Rev Musculoskelet Med JT - Current reviews in musculoskeletal medicine JID - 101317803 PMC - PMC13714233 OTO - NOTNLM OT - Adult scoliosis OT - Anterior column realignment OT - Circumferential minimally invasive surgery OT - Lateral lumbar interbody fusion OT - Patient-specific implants OT - Single-position surgery COIS- Declarations. Human and Animal Rights and Informed Consent: This article does not contain any studies with human or animal subjects performed by any of the authors. Competing interest: The authors did not receive support from any organization for the submitted work. Dr. Khan and Mr. Hoofnagle declare that they have no conflicts of interest. Dr. Chou reports personal fees from Globus and Medtronic, outside the submitted work. Dr. Mummaneni reports personal fees from DePuy, Johnson & Johnson MedTech, Globus, GE/BK Medical, and Medtronic; grants from NREF, ISSG, AO Spine, NIH, Pacira, and DoD; stock in Discgenics; and royalties from Thieme and Springer, outside the submitted work. Dr. Chan reports personal fees from Alphatec Spine Inc., Isto Biologics, Carlsmed, and SpineArt, outside the submitted work. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 12:06 PHST- 2026/09/18 00:00 [received] PHST- 2026/10/21 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:06 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 10.1007/s12178-026-10064-5 [pii] AID - 10064 [pii] AID - 10.1007/s12178-026-10064-5 [doi] PST - epublish SO - Curr Rev Musculoskelet Med. 2026 Oct 27;19(1):71. doi: 10.1007/s12178-026-10064-5. PMID- 42944790 OWN - NLM STAT- Publisher LR - 20261027 IS - 1434-9949 (Electronic) IS - 0770-3198 (Linking) DP - 2026 Oct 27 TI - Genetic liability to systemic lupus erythematosus and autoimmune thyroid diseases: evidence from a bidirectional mendelian randomization study. LID - 10.1007/s10067-026-08492-2 [doi] AB - BACKGROUND: Previous observational studies imply a potential link between systemic lupus erythematosus (SLE) and autoimmune thyroid disease (AITD), although they are susceptible to confounding factors. This study aims to assess the genetic evidence for a potential causal association between SLE and AITD using a bidirectional and multivariable two-sample Mendelian randomization (MR) analysis. METHODS: We retrieved datasets for systemic lupus erythematosus (SLE), autoimmune thyroiditis (AT), autoimmune hyperthyroidism, and autoimmune hypothyroidism from European population-based databases, and data for Graves' disease (GD) from Biobank Japan (BBJ). Instrumental variables (IVs) related to exposure were selected for the Mendelian randomization (MR) analysis. Sensitivity tests, including horizontal pleiotropy and heterogeneity assessments, were conducted to ensure result reliability, and the analysis outcomes were visualized. Bonferroni correction was applied to the 8 primary exposure-outcome pairs (corrected significance threshold p < 0.00625). The use of a Japanese GWAS dataset for GD alongside predominantly European datasets for other phenotypes represents a methodological limitation due to potential cross-ancestry differences in linkage disequilibrium structure and allele frequencies. RESULTS: Genetic variants linked to SLE, AT, GD, autoimmune hyperthyroidism, and autoimmune hypothyroidism were utilized as IVs. MR analysis was performed using data from 5201 SLE cases and 9066 controls; 244 AT cases and 187,684 controls; 2176 GD cases and 210,277 controls; 962 autoimmune hyperthyroidism cases and 172,976 controls; 22,997 autoimmune hypothyroidism cases and 175,475 controls. In the primary IVW analysis, genetic liability to SLE was associated with increased risks of AT (OR = 1.136, 95% CI 1.006-1.284, p = 0.040) and autoimmune hypothyroidism (OR = 1.036, 95% CI 1.004-1.070, p = 0.025). Genetically predicted autoimmune hyperthyroidism is associated with an increased risk of SLE in both univariable (OR = 1.905, 95% CI 1.195-3.037, p = 0.007) and multivariable (OR = 1.381, 95% CI 1.042-1.830, p = 0.024) MR analyses. However, these associations did not remain statistically significant after Bonferroni correction for 8 tested exposure-outcome pairs (corrected significance threshold: p < 0.00625). Sensitivity analysis showed limited evidence of horizontal pleiotropy, though significant heterogeneity was detected for several exposure-outcome pairs (see Fig. 5). CONCLUSION: Mendelian randomization analysis suggests that genetic liability to SLE is associated with a potential increased risk of AT and autoimmune hypothyroidism, and genetic liability to autoimmune hyperthyroidism is associated with a possible increased risk of SLE in European populations. These findings are based on genetic instruments and should be interpreted as genetic evidence consistent with potential causal effects rather than definitive proof of causality. The observed associations have relatively modest effect sizes and reflect lifelong genetic liability; they should not be equated with the clinical epidemiological risk among patients with established disease. Their clinical relevance requires further validation in large-scale prospective clinical studies. The mixed ancestry of GWAS datasets and the presence of significant heterogeneity are important limitations. Key Points * This study systematically evaluated the bidirectional genetic associations between SLE and distinct AITD subtypes via a comprehensive bidirectional and multivariable two-sample MR design. * Genetic liability to SLE was genetically associated with an increased risk of AT and autoimmune hypothyroidism in European populations. * Autoimmune hyperthyroidism was identified as a potential genetic risk factor for SLE, validated by both univariable and multivariable MR analyses. * The findings provide genetic evidence for understanding the shared genetic architecture and potential causal associations between SLE and AITD subtypes. However, given the modest effect sizes and the distinction between genetic liability and clinical disease, they do not currently support routine targeted thyroid comorbidity screening in all SLE patients without further clinical validation. CI - (c) 2026. The Author(s), under exclusive licence to International League of Associations for Rheumatology (ILAR). FAU - Zhang, Jiali AU - Zhang J AD - The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, China. AD - School of Clinical Medicine, Qilu Medical University, Zibo, China. FAU - Duan, Mingfei AU - Duan M AD - The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, China. AD - Zhuhai People's Hospital (The Affiliated Hospital of Beijing Institute of Technology, Zhuhai Clinical Medical College of Jinan University), Zhuhai, Guangdong, China. FAU - Wu, Shaohong AU - Wu S AD - The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, China. FAU - Li, Kengtong AU - Li K AD - The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, China. FAU - Wang, Cunchuan AU - Wang C AD - The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, China. twcc@jnu.edu.cn. AD - The Third Affiliated Hospital of South China University of Technology, General Surgery, Guangzhou, China. twcc@jnu.edu.cn. FAU - Zhang, Jinshan AU - Zhang J AUID- ORCID: 0009-0006-2201-9476 AD - The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, China. zhangjinshandoctor@163.com. LA - eng GR - 202201020063/the Science and Technology Projects in Guangzhou/ GR - 711003/the flagship specialty construction project-General surgery of The First Affiliated Hospital of Jinan University/ GR - 82405211/National Natural Science Foundation of China/ GR - 82601637/National Natural Science Foundation of China/ GR - 2026M792238/General Grant of the China Postdoctoral Science Foundation/ PT - Journal Article DEP - 20261027 PL - Germany TA - Clin Rheumatol JT - Clinical rheumatology JID - 8211469 SB - IM OTO - NOTNLM OT - Autoimmune thyroid diseases OT - Mendelian randomization OT - Systemic lupus erythematosus COIS- Compliance with ethical standards. Ethics approval: Statement of Ethical Approval this study used only publicly available data, and ethical approval was obtained in the original study ( https://gwas.mrcieu.ac.uk/ ). This study used only publicly available, de-identified summary-level GWAS data. No original patient data were collected. Ethical approval for the original GWAS studies was obtained by the respective consortiums (EBI, FinnGen, UK Biobank, and Biobank Japan). Disclosures: None. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:06 PHST- 2026/05/11 00:00 [received] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/09/21 00:00 [revised] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:06 [entrez] AID - 10.1007/s10067-026-08492-2 [pii] AID - 10.1007/s10067-026-08492-2 [doi] PST - aheadofprint SO - Clin Rheumatol. 2026 Oct 27. doi: 10.1007/s10067-026-08492-2. PMID- 42944786 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1432-1068 (Electronic) IS - 1633-8065 (Print) IS - 1633-8065 (Linking) VI - 36 IP - 1 DP - 2026 Oct 27 TI - Association of bone mineral-reducing medications with two-year complications following open reduction and internal fixation of ankle fractures: a propensity score-matched analysis. LID - 10.1007/s00590-026-05009-0 [doi] LID - 427 AB - BACKGROUND: Bone mineral-reducing medications (BMRMs) are commonly prescribed, but their association with outcomes after ankle open reduction and internal fixation (ORIF) is unclear. We evaluated preoperative exposure to ten BMRM classes and two-year complications after ankle ORIF. METHODS: Adults undergoing ankle ORIF at least two years before data extraction were identified in TriNetX. Exposure required at least one medication record 1-180 days before surgery for proton pump inhibitors (PPIs), glucocorticoids, loop diuretics, thiazolidinediones, calcineurin inhibitors, aromatase inhibitors, selective serotonin reuptake inhibitors (SSRIs), first- or second-generation antipsychotics, or antiepileptics. Exposed patients were 1:1 propensity score matched to patients unexposed to all classes. Risk ratios (RRs) for five two-year outcomes were adjusted for false discovery rate (FDR). RESULTS: Matched cohorts included 227-9,858 patients per group. Nonunion was more frequent with glucocorticoids (2.36% vs. 1.72%; RR 1.37), PPIs (2.68% vs. 1.79%; RR 1.50), loop diuretics (3.98% vs. 2.20%; RR 1.81), SSRIs (2.49% vs. 1.72%; RR 1.45) and antiepileptics (2.70% vs. 1.73%; RR 1.56); all 95% confidence intervals excluded 1 and all FDR q /=20% relative decline in PW from baseline to a fixed 24 month assessment among patients with evaluable paired baseline and 24 month measurements. Factors associated with this endpoint were explored using logistic regression.A three-component exploratory PDRS and a baseline-PW-excluded sensitivity PDRS were evaluated for descriptive risk stratification. Clinical stent failure was analyzed separately using a competing-risk framework with death treated as a competing event. RESULTS: During longitudinal follow-up, eGFR and PW showed different trajectories, with relatively stable eGFR during early and intermediate follow-up and a progressive decrease in PW. Among 61 patients with evaluable paired baseline and 24 month PW measurements, 32 (52.5%) had a >/=20% PW decline. Median absolute PW reduction at 24 months was 3.65 mm. In exploratory multivariable analysis, greater baseline affected-side PW remained associated with >/=20% PW decline (adjusted OR, 26.80,95% CI 3.58-200.57, P=0.001), whereas bladder invasion and PLaCT score were not statistically significant after adjustment. The main exploratory PDRS showed moderate discrimination (apparent AUC, 0.786,bootstrap 95% CI 0.677-0.877,mean bootstrap optimism, 0.0224,bootstrap optimism-corrected AUC, 0.763), whereas the baseline-PW-excluded sensitivity PDRS showed lower discrimination (apparent AUC, 0.658,bootstrap 95% CI 0.538-0.772). Clinical stent failure occurred in 35 patients (35.4%),the cumulative incidence was 30.8% at 24 months and 49.7% at 60 months. CONCLUSIONS: In patients with MUO managed with ureteral stents, renal function and renal parenchymal width showed distinct longitudinal patterns. PW decreased over time despite relatively stable eGFR during early and intermediate follow-up, suggesting that structural imaging measures may provide information complementary to serum-based renal function assessment. The >/=20% PW-decline endpoint and PDRS should be interpreted as exploratory, and the findings do not establish that ureteral stenting preserved renal function or caused, prevented, or attenuated parenchymal change. CI - (c) 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Huang, Qianhao AU - Huang Q AD - The Key Laboratory of Urinary Tract Tumours and Calculi, Department of Urology, School of Medicine, The First Affiliated Hospital of Xiamen University, Xiamen University, Xiamen, 361003, Fujian, China. AD - Department of Urology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China. FAU - Huang, Yifan AU - Huang Y AD - The Key Laboratory of Urinary Tract Tumours and Calculi, Department of Urology, School of Medicine, The First Affiliated Hospital of Xiamen University, Xiamen University, Xiamen, 361003, Fujian, China. AD - The Graduate School of Fujian Medical University, Fuzhou, 350122, China. FAU - Han, Bingzhi AU - Han B AD - The Key Laboratory of Urinary Tract Tumours and Calculi, Department of Urology, School of Medicine, The First Affiliated Hospital of Xiamen University, Xiamen University, Xiamen, 361003, Fujian, China. FAU - Lian, Xin AU - Lian X AD - The Key Laboratory of Urinary Tract Tumours and Calculi, Department of Urology, School of Medicine, The First Affiliated Hospital of Xiamen University, Xiamen University, Xiamen, 361003, Fujian, China. AD - Department of Urology, Lishui Municipal Central Hospital, Lishui, 323000, Zhejiang, China. FAU - Huang, Zikai AU - Huang Z AD - The Key Laboratory of Urinary Tract Tumours and Calculi, Department of Urology, School of Medicine, The First Affiliated Hospital of Xiamen University, Xiamen University, Xiamen, 361003, Fujian, China. AD - The Graduate School of Fujian Medical University, Fuzhou, 350122, China. FAU - Wang, Tao AU - Wang T AD - The Key Laboratory of Urinary Tract Tumours and Calculi, Department of Urology, School of Medicine, The First Affiliated Hospital of Xiamen University, Xiamen University, Xiamen, 361003, Fujian, China. taowang@xmu.edu.cn. AD - The Graduate School of Fujian Medical University, Fuzhou, 350122, China. taowang@xmu.edu.cn. FAU - Chen, Bin AU - Chen B AD - The Key Laboratory of Urinary Tract Tumours and Calculi, Department of Urology, School of Medicine, The First Affiliated Hospital of Xiamen University, Xiamen University, Xiamen, 361003, Fujian, China. chenbin5557@163.com. AD - The Graduate School of Fujian Medical University, Fuzhou, 350122, China. chenbin5557@163.com. FAU - Chen, Yuedong AU - Chen Y AD - The Key Laboratory of Urinary Tract Tumours and Calculi, Department of Urology, School of Medicine, The First Affiliated Hospital of Xiamen University, Xiamen University, Xiamen, 361003, Fujian, China. chenyuedong8@126.com. LA - eng GR - 2022J011353/Fujian Provincial Health Technology Project/ PT - Journal Article DEP - 20261027 PL - Germany TA - World J Urol JT - World journal of urology JID - 8307716 SB - IM MH - Humans MH - *Ureteral Obstruction/etiology/surgery/physiopathology MH - *Stents MH - Female MH - Retrospective Studies MH - Male MH - Aged MH - *Kidney/diagnostic imaging/physiopathology/pathology MH - Middle Aged MH - *Glomerular Filtration Rate MH - Longitudinal Studies MH - *Ureteral Neoplasms/complications MH - Ureter OTO - NOTNLM OT - Malignant ureteral obstruction OT - Renal parenchymal width OT - Renal structural deterioration OT - Risk stratification OT - Stent failure OT - Ureteral stenting COIS- Declarations. Conflict of interest: The authors declare that the study was conducted in the absence of any business or financial relationship that could be interpreted as a potential conflict of interest. Ethical approval: This study was reviewed and approved by the Ethics Committee of the First Affiliated Hospital of Xiamen University. The requirement for informed consent was waived because of the retrospective nature of the study. All data were anonymized before analysis. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 12:03 PHST- 2026/06/21 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:03 [entrez] AID - 10.1007/s00345-026-06875-3 [pii] AID - 10.1007/s00345-026-06875-3 [doi] PST - epublish SO - World J Urol. 2026 Oct 27;44(1):764. doi: 10.1007/s00345-026-06875-3. PMID- 42944756 OWN - NLM STAT- Publisher LR - 20261027 IS - 1558-8238 (Electronic) IS - 0021-9738 (Linking) DP - 2026 Oct 27 TI - Donor-derived CD8+CD122+ Tregs generated in mixed donor chimeric NOD mice suppress autoreactive T cells. LID - e207024 [pii] LID - 10.1172/JCI207024 [doi] AB - The establishment of mixed hematopoietic chimerism is a promising way to induce immune tolerance for islet replacement therapy and to treat the underlying autoimmunity in type 1 diabetes (T1D). Mixed chimerism not only promotes effective thymic negative selection of autoreactive cells but also restores regulatory T cell (Treg) function and peripheral tolerance. In the current study, we determined that a novel class of donor-derived CD8+CD44+CD122+ Tregs (d-CD8+CD122+ Tregs) plays a crucial role in controlling autoimmunity in non-obese diabetic (NOD) mice with induced mixed chimerism. Using adoptive T cell transfer experiments, we showed that d-CD8+CD122+ Tregs abrogate autoimmunity by suppressing the exogenously injected diabetogenic T cells in Recombination-Activating Gene deficient NOD mice. These d-CD8+CD122+ Tregs from NOD chimeras show upregulation of Helios, Programmed cell death protein 1, perforin, granzyme-B, CD39, Folate receptor 4, and downregulation of proinflammatory marker SCART1. Using in vitro assays, we show that d-CD8+CD122+ Tregs respond specifically to a Complementarity-Determining Region-3 peptide sequence derived from T cell receptors of islet antigen-specific autoreactive T cells. Similarly, we found that individuals with T1D have a deficiency in CD8+CD122+ Tregs, suggesting a potential loss of regulatory function accompanies disease onset. Revitalizing CD8+CD122+ Tregs may offer a new therapeutic strategy of restoring immune tolerance in autoimmune diabetes. FAU - Pathak, Shiva AU - Pathak S AD - Division of Blood and Marrow Transplantation and Cellular Therapy, Stanford University School of Medicine, Stanford, United States of America. FAU - Bader, Cameron S AU - Bader CS AD - Division of Blood and Marrow Transplantation and Cellular Therapy, Stanford University School of Medicine, Stanford, United States of America. FAU - Iliopoulou, Bettina P AU - Iliopoulou BP AD - Division of Blood and Marrow Transplantation and Cellular Therapy, Stanford University School of Medicine, Stanford, United States of America. FAU - Regmi, Shobha AU - Regmi S AD - Department of Pediatric Gastroenterology, Stanford University School of Medicine, Stanford, United States of America. FAU - Chen, Pin-I AU - Chen PI AD - Division of Blood and Marrow Transplantation and Cellular Therapy, Stanford University School of Medicine, Stanford, United States of America. FAU - Gupta, Biki AU - Gupta B AD - Division of Blood and Marrow Transplantation and Cellular Therapy, Stanford University School of Medicine, Stanford, United States of America. FAU - Wu, Xiangni AU - Wu X AD - Division of Blood and Marrow Transplantation and Cellular Therapy, Stanford University School of Medicine, Stanford, United States of America. FAU - Mosher, Blake AU - Mosher B AD - Division of Blood and Marrow Transplantation and Cellular Therapy, Stanford University School of Medicine, Stanford, United States of America. FAU - Wells, Alexandra AU - Wells A AD - Division of Blood and Marrow Transplantation and Cellular Therapy, Stanford University School of Medicine, Stanford, United States of America. FAU - Witherspoon, Levon AU - Witherspoon L AD - Division of Blood and Marrow Transplantation and Cellular Therapy, Stanford University School of Medicine, Stanford, United States of America. FAU - Jenkins, Kayla C AU - Jenkins KC AD - Division of Blood and Marrow Transplantation and Cellular Therapy, Stanford University School of Medicine, Stanford, United States of America. FAU - Harper, William AU - Harper W AD - Division of Blood and Marrow Transplantation and Cellular Therapy, Stanford University School of Medicine, Stanford, United States of America. FAU - SooHoo, Emily Y AU - SooHoo EY AD - Division of Blood and Marrow Transplantation and Cellular Therapy, Stanford University School of Medicine, Stanford, United States of America. FAU - Twoy, Abigail AU - Twoy A AD - Division of Blood and Marrow Transplantation and Cellular Therapy, Stanford University School of Medicine, Stanford, United States of America. FAU - Ahmed, Rizwan AU - Ahmed R AD - Department of Immunology and Rheumatology, Stanford University School of Medicine, Stanford, United States of America. FAU - Dutt, Suparna AU - Dutt S AD - Department of Radiation Oncology, Stanford University School of Medicine, Stanford, United States of America. FAU - Nagy, Nadine AU - Nagy N AD - Division of Blood and Marrow Transplantation and Cellular Therapy, Stanford University School of Medicine, Stanford, United States of America. FAU - Jensen, Kent P AU - Jensen KP AD - Division of Blood and Marrow Transplantation and Cellular Therapy, Stanford University School of Medicine, Stanford, United States of America. FAU - Fathman, C Garrison AU - Fathman CG AD - Department of Immunology and Rheumatology, Stanford University School of Medicine, Stanford, United States of America. FAU - Thakor, Avnesh S AU - Thakor AS AD - Department of Radiology, Stanford University School of Medicine, Stanford, United States of America. FAU - Davis, Mark M AU - Davis MM AD - Institute of Immunity, Transplantation and Infection, Stanford University School of Medicine, Stanford, United States of America. FAU - Meyer, Everett H AU - Meyer EH AD - Division of Blood and Marrow Transplantation and Cellular Therapy, Stanford University School of Medicine, Stanford, United States of America. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - J Clin Invest JT - The Journal of clinical investigation JID - 7802877 SB - IM OTO - NOTNLM OT - Autoimmune diseases OT - Autoimmunity OT - Diabetes OT - Immunology OT - Tregs EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 12:03 PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 12:03 [entrez] AID - 207024 [pii] AID - 10.1172/JCI207024 [doi] PST - aheadofprint SO - J Clin Invest. 2026 Oct 27:e207024. doi: 10.1172/JCI207024. PMID- 42944735 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2057-5858 (Electronic) IS - 2057-5858 (Linking) VI - 12 IP - 10 DP - 2026 Oct TI - The genomic diversity of Salmonella Enteritidis from poultry and human clinical cases in Israel. LID - 10.1099/mgen.0.001878 [doi] LID - 001878 AB - Salmonella Enteritidis, a leading cause of foodborne illness worldwide, is often linked to poultry. Despite the limited genetic variation of the serotype, distinct lineages have been shown to circulate at both national and global scales, and invasive and antimicrobial-resistant strains have emerged. In Israel, despite active surveillance and mitigation measures in the poultry sector, the pathogen persists in commercial layer hen flocks and continues to cause human clinical infections. We aimed to characterize the genetic diversity and antimicrobial resistance (AMR) determinants of S. Enteritidis isolates from poultry and human clinical cases in Israel and to compare these findings with the genetic diversity of this serotype found globally. Whole-genome sequencing was performed on S. Enteritidis isolates from surveillance samples collected from layer hen flocks (n=48) and human clinical cases (n=29) between 2019 and 2022 and compared to a representative global dataset of sequences (n=115). We identified a genetically diverse S. Enteritidis population in Israel. A dominant clade I and a smaller clade IV were ST11 and closely related to each of the major 'Atlantic' and 'Global' lineages circulating globally. These clades demonstrated shared circulation between poultry and human populations which may suggest poultry-associated transmission. In addition, three smaller distinct Israeli clades were not part of the global clades. These included clades II and III of ST11 and clade V of ST183, a rarely described sequence type in poultry. Mutations in the gyrA gene (D87G, D87Y and S83F) conferring resistance to fluoroquinolones were present in both human (6.9%) and poultry (10.4%) isolates from Israel, highlighting the importance of continued antimicrobial resistance surveillance in this serotype. These findings establish a genomic baseline to support control and mitigation policy for S. Enteritidis in Israel and contribute to the understanding of its genomic diversity globally. FAU - Perry, Janet AU - Perry J AD - The Koret School of Veterinary Medicine, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Jerusalem, Israel. FAU - Arnold, Katya AU - Arnold K AD - The Koret School of Veterinary Medicine, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Jerusalem, Israel. FAU - Wiseman, Anat AU - Wiseman A AD - Poultry Health Laboratories, Egg and Poultry Board, Kiryat Malachi, Israel. FAU - Markus, Yael AU - Markus Y AD - Poultry Health Laboratories, Egg and Poultry Board, Kiryat Malachi, Israel. FAU - From, Ido AU - From I AD - Poultry Health Laboratories, Egg and Poultry Board, Kiryat Malachi, Israel. FAU - Yakunin, Eugenia AU - Yakunin E AD - Public Health Laboratories - Jerusalem, Ministry of Health, Jerusalem, Israel. FAU - Rokney, Assaf AU - Rokney A AD - Public Health Laboratories - Jerusalem, Ministry of Health, Jerusalem, Israel. FAU - Elnekave, Ehud AU - Elnekave E AD - The Koret School of Veterinary Medicine, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Jerusalem, Israel. LA - eng PT - Journal Article PL - England TA - Microb Genom JT - Microbial genomics JID - 101671820 RN - 0 (Anti-Bacterial Agents) SB - IM MH - Animals MH - *Salmonella enteritidis/genetics/isolation & purification/classification/drug effects MH - Humans MH - Israel/epidemiology MH - *Genetic Variation MH - Chickens/microbiology MH - Whole Genome Sequencing MH - *Poultry Diseases/microbiology MH - Phylogeny MH - Genome, Bacterial MH - Poultry/microbiology MH - Anti-Bacterial Agents/pharmacology MH - *Salmonella Infections, Animal/microbiology MH - Drug Resistance, Bacterial PMC - PMC13712976 OTO - NOTNLM OT - Salmonellosis OT - antimicrobial resistance OT - poultry OT - public health OT - zoonosis COIS- The authors declare that there are no conflicts of interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 11:53 PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:53 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 001878 [pii] AID - 10.1099/mgen.0.001878 [doi] PST - ppublish SO - Microb Genom. 2026 Oct;12(10):001878. doi: 10.1099/mgen.0.001878. PMID- 42944734 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1471-4159 (Electronic) IS - 0022-3042 (Print) IS - 0022-3042 (Linking) VI - 170 IP - 10 DP - 2026 Oct TI - Examining the Impact of Neurodevelopmental Nicotine Exposure on Cortical Excitation/Inhibition Homeostasis Markers in a Cerebral Organoid Model of Schizophrenia. LID - e70548 [pii] LID - 10.1111/jnc.70548 [doi] AB - Schizophrenia (SCZ) is a severe neuropsychiatric disorder thought to arise from an interplay between genetic susceptibility and environmental risk factors, including prenatal nicotine exposure (PNE). Although PNE has been associated with increased risk for SCZ, the underlying mechanisms remain poorly understood. Both SCZ and PNE have independently been linked to excitation/inhibition (E/I) imbalance, suggesting that PNE may exacerbate neurodevelopmental disruptions associated with SCZ. This study employed a cerebral organoid model derived from human induced pluripotent stem cells to examine the impact of PNE on E/I-related pathways in SCZ. Organoids were chronically exposed to nicotine and analyzed using immunofluorescence and quantitative polymerase chain reaction. Results revealed disease-associated differences between SCZ and control organoids in a neural progenitor cell marker and several GABAergic, glutamatergic and cholinergic markers. Specifically, SCZ-derived organoids showed reduced SOX2 and GABAergic differentiation and synthesis markers (LHX6, GAD1/GAD67 and SST), increased select GABAergic and glutamatergic markers (GABRA1, GABRA2, GABBR2 and GRIA1), and alterations in multiple cholinergic synthesis, degradation and receptor markers. Effects of nicotine were more modest and limited to CCK, SLC6A1 and SST. These findings support a role for E/I imbalance in SCZ pathophysiology and offer insights into how early-life exposures may influence disease risk. CI - (c) 2026 The Author(s). Journal of Neurochemistry published by John Wiley & Sons Ltd on behalf of International Society for Neurochemistry. FAU - Zhaksylyk, Kuralay AU - Zhaksylyk K AUID- ORCID: 0009-0000-5355-8663 AD - Addiction Research Group, University of Western Ontario, London, Ontario, Canada. AD - Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario, Canada. FAU - Sarikahya, Mohammed H AU - Sarikahya MH AUID- ORCID: 0000-0002-5511-2638 AD - Addiction Research Group, University of Western Ontario, London, Ontario, Canada. AD - Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario, Canada. FAU - Perez-Valenzuela, Enzo AU - Perez-Valenzuela E AUID- ORCID: 0000-0002-0906-2123 AD - Addiction Research Group, University of Western Ontario, London, Ontario, Canada. AD - Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario, Canada. FAU - Hardy, Daniel B AU - Hardy DB AUID- ORCID: 0000-0001-5445-273X AD - Lawson Health Research Institute, University of Western Ontario, London, Ontario, Canada. AD - Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada. AD - Division of Maternal, Fetal and Newborn Health, Childrens Health Research Institute (CHRI), University of Western Ontario, London, Ontario, Canada. FAU - Rushlow, Walter J AU - Rushlow WJ AD - Addiction Research Group, University of Western Ontario, London, Ontario, Canada. AD - Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario, Canada. AD - Lawson Health Research Institute, University of Western Ontario, London, Ontario, Canada. FAU - Laviolette, Steven R AU - Laviolette SR AUID- ORCID: 0000-0001-5410-7389 AD - Addiction Research Group, University of Western Ontario, London, Ontario, Canada. AD - Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario, Canada. AD - Lawson Health Research Institute, University of Western Ontario, London, Ontario, Canada. AD - Department of Psychiatry, University of Western Ontario, London, Ontario, Canada. LA - eng GR - MOP-189943/CAPMC/CIHR/Canada GR - Province of Ontario/ GR - Natural Sciences and Engineering Research Council of Canada/ PT - Journal Article PL - England TA - J Neurochem JT - Journal of neurochemistry JID - 2985190R RN - 6M3C89ZY6R (Nicotine) RN - 0 (Biomarkers) SB - IM MH - *Nicotine/toxicity MH - Humans MH - *Schizophrenia/metabolism/chemically induced/pathology MH - *Organoids/drug effects/metabolism MH - *Homeostasis/drug effects/physiology MH - *Cerebral Cortex/drug effects/metabolism MH - Female MH - Neurodevelopment MH - Pregnancy MH - Biomarkers/metabolism MH - *Neural Inhibition/drug effects/physiology MH - Induced Pluripotent Stem Cells/drug effects/metabolism PMC - PMC13712444 OTO - NOTNLM OT - GABA OT - acetylcholine OT - cerebral organoids OT - glutamate OT - immunofluorescence OT - prenatal nicotine exposure OT - qPCR OT - schizophrenia COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 11:53 PHST- 2026/07/29 00:00 [revised] PHST- 2026/03/05 00:00 [received] PHST- 2026/08/18 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:53 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e70548 [pii] AID - JNC70548 [pii] AID - 10.1111/jnc.70548 [doi] PST - ppublish SO - J Neurochem. 2026 Oct;170(10):e70548. doi: 10.1111/jnc.70548. PMID- 42944732 OWN - NLM STAT- Publisher LR - 20261027 IS - 1756-8927 (Electronic) IS - 1756-8919 (Linking) DP - 2026 Oct 27 TI - Isoform-selective FGFR inhibition as pioneer in cancer therapy: heterocyclic scaffold optimization strategies, mechanistic insights, and future directions. PG - 1-19 LID - 10.1080/17568919.2026.2748642 [doi] AB - INTRODUCTION: Fibroblast Growth Factor Receptor (FGFR) drives cell proliferation by triggering PI3K-AKT, JAK-STAT, MAPK, IP3-DAG, and RAS-RAF-MEK cascades, promoting lung, breast, prostate, and bladder cancers. Pan-selective FGFR inhibitors (SFGFRIs) are characterized by their ability to target multiple FGFR isoforms (FGFR1-4). Several heterocyclic pan-SFGFRIs have been approved by the FDA. Their lack of isoform-selectivity has raised adverse effects such as hyperphosphatemia, ocular and gastrointestinal toxicities, nail toxicities, and fatigue. Therefore, FGFR (1/2/3/4)-isoform-selective heterocyclic-FGFRIs have emerged as promising alternatives to reduce off-target effects and overcome drug resistance. AREAS COVERED: This review highlights mechanisms, advantages, limitations, and drug resistance of FDA-approved and clinical SFGFRIs. The in silico isoform-selectivity study provided insights into isoform-specific binding affinity, interactions, and mechanisms of SFGFRIs. Screening, classification, and scaffold-oriented structure-optimization strategies of promising heterocyclic SFGFRIs provided future-direction toward isoform-selectivity. EXPERT OPINION: Structure-activity-correlation study revealed pyrazole and indazole as FGFR1-targeted, pyridine and pyrimidine as dual FGFR1-2-targeted, and quinazoline as FGFR4-targeted privileged scaffolds. In silico evaluation highlighted the scarcity of pure FGFR1-selective inhibitors, while isoform-selectivity was demonstrated by resigratinib toward FGFR2 (-14.008 kcal/mol), tasurgratinib toward FGFR3 (-4.634 kcal/mol), and H3B-6527 toward FGFR4 (-5.162 kcal/mol). Collectively, the findings underscored isoform-selective FGFR inhibition as a vast avenue for exploration to deliver a paradigm-shift in oncology by addressing drug resistance and minimizing toxicities. FAU - Das, Agnidipta AU - Das A AUID- ORCID: 0000-0001-7689-0298 AD - Department of Pharmaceutical Sciences and Natural Products, Central University of Punjab, Bathinda, India. FAU - Purohit, Nehal AU - Purohit N AUID- ORCID: 0000-0003-2528-6722 AD - Department of Pharmaceutical Sciences and Natural Products, Central University of Punjab, Bathinda, India. FAU - Kumar, Amit AU - Kumar A AUID- ORCID: 0000-0001-7850-4936 AD - Department of Pharmaceutical Sciences and Natural Products, Central University of Punjab, Bathinda, India. AD - Department of Pharmaceutical Sciences, Chitkara University, Rajpura, India. FAU - Jaitak, Vikas AU - Jaitak V AUID- ORCID: 0000-0002-2811-5251 AD - Department of Pharmaceutical Sciences and Natural Products, Central University of Punjab, Bathinda, India. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - England TA - Future Med Chem JT - Future medicinal chemistry JID - 101511162 SB - IM OTO - NOTNLM OT - Isoform-selective OT - cancer OT - inhibition OT - mechanisms OT - resistance OT - strategy EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 11:53 PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:53 [entrez] AID - 10.1080/17568919.2026.2748642 [doi] PST - aheadofprint SO - Future Med Chem. 2026 Oct 27:1-19. doi: 10.1080/17568919.2026.2748642. PMID- 42944722 OWN - NLM STAT- Publisher LR - 20261027 IS - 1460-2105 (Electronic) IS - 0027-8874 (Linking) DP - 2026 Oct 27 TI - Carcinogen exposure, TP53, and clonal selection in the oral cavity. LID - djag381 [pii] LID - 10.1093/jnci/djag381 [doi] FAU - Sikora, Andrew G AU - Sikora AG AUID- ORCID: 0000-0002-8357-8435 AD - Department of Head and Neck Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX, United States. AD - Department of Immunology, The University of Texas MD Anderson Cancer Center, Houston, TX, United States. FAU - Anderson, Jennifer L AU - Anderson JL AD - Department of Head and Neck Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX, United States. FAU - Zhao, Xiao AU - Zhao X AD - Department of Head and Neck Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX, United States. AD - Department of Genetics, The University of Texas MD Anderson Cancer Center, Houston, TX, United States. FAU - Lei, Yu Leo AU - Lei YL AD - Department of Head and Neck Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX, United States. AD - Department of Cancer Biology, The University of Texas MD Anderson Cancer Center, Houston, TX, United States. AD - Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, United States. LA - eng GR - RP250398/Cancer Prevention and Research Institute of Texas (CPRIT)/ GR - R01 CA269516/NH/NIH HHS/United States GR - R01 DE030875/NH/NIH HHS/United States GR - R01 DE026728/NH/NIH HHS/United States GR - R01 DE031951/NH/NIH HHS/United States GR - U01 DE033330/NH/NIH HHS/United States PT - Comment PT - Editorial DEP - 20261027 PL - United States TA - J Natl Cancer Inst JT - Journal of the National Cancer Institute JID - 7503089 SB - IM CON - J Natl Cancer Inst. 2026 Aug 13:djag267. doi: 10.1093/jnci/djag267. PMID: 42593222 EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 11:52 PHST- 2026/09/16 00:00 [received] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:52 [entrez] AID - 9003128 [pii] AID - 10.1093/jnci/djag381 [doi] PST - aheadofprint SO - J Natl Cancer Inst. 2026 Oct 27:djag381. doi: 10.1093/jnci/djag381. PMID- 42944719 OWN - NLM STAT- Publisher LR - 20261027 IS - 1521-3773 (Electronic) IS - 1433-7851 (Linking) DP - 2026 Oct 27 TI - Hierarchically Gated DNA Nanoswitches Enable Context-Selective Activation of Antimicrobial Peptides. LID - e6007035 [pii] LID - 10.1002/anie.6007035 [doi] AB - Precise control over cytolytic peptide activity remains a central challenge in antimicrobial therapy because membrane disruption is intrinsically coupled with systemic toxicity. Here we encode a hierarchically gated molecular logic within a DNA nanoswitch to enable context-selective activation of melittin only at infection sites. The nanoswitch integrates three cooperative control layers: bacterial tropism, microenvironmental sensing, and inflammation-associated enzymatic activation. Maltodextrin ligands are spatially programmed to engage the bacteria-specific maltodextrin transporter, yielding selective bacterial recognition with a 2.3-order-of-magnitude enhancement in binding. Cytosine-rich i-motif locks convert acidic biofilm conditions into conformational opening of the scaffold, while a caspase-1-cleavable peptide tether provides a final enzymatic gate under inflammatory cues. This hierarchical gating confines membrane-lytic activity with high spatiotemporal precision and suppresses off-target hemolysis and systemic leakage. In uropathogenic Escherichia coli (UPEC)-associated urinary tract infection models, the nanoswitch achieves effective bacterial clearance comparable to that of ciprofloxacin in vivo, demonstrating a generalizable strategy for conditional peptide activation. CI - (c) 2026 Wiley-VCH GmbH. FAU - Xie, Ruao AU - Xie R AD - Institute of Functional Nano & Soft Materials, Jiangsu Key Laboratory of Advanced Healthcare Materials & Devices, Soochow University, Suzhou, China. FAU - Ling, Yufan AU - Ling Y AUID- ORCID: 0009-0005-1475-7703 AD - School of Radiation Medicine and Protection, Suzhou Medical College of Soochow University, Suzhou, China. FAU - Lu, Xing AU - Lu X AUID- ORCID: 0009-0006-7192-0770 AD - Institute of Functional Nano & Soft Materials, Jiangsu Key Laboratory of Advanced Healthcare Materials & Devices, Soochow University, Suzhou, China. FAU - Li, Peng AU - Li P AD - The Fourth Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. FAU - Yu, Xiaojun AU - Yu X AD - The Fourth Affiliated Hospital of Soochow University, Suzhou, Jiangsu, China. FAU - He, Yao AU - He Y AUID- ORCID: 0000-0003-1672-4057 AD - Institute of Functional Nano & Soft Materials, Jiangsu Key Laboratory of Advanced Healthcare Materials & Devices, Soochow University, Suzhou, China. AD - Macao Translational Medicine Center, Macau University of Science and Technology, Taipa, Macau SAR, China. FAU - Wang, Houyu AU - Wang H AUID- ORCID: 0000-0002-5134-9881 AD - Institute of Functional Nano & Soft Materials, Jiangsu Key Laboratory of Advanced Healthcare Materials & Devices, Soochow University, Suzhou, China. LA - eng GR - 2023YFB3208200/National Key Research and Development Program of China/ GR - 22393932/National Natural Science Foundation of China/ GR - T2321005/National Natural Science Foundation of China/ GR - 0002/2022/AKP/Science and Technology Development Fund, Macau SAR/ GR - 0115/2023/RIA2/Science and Technology Development Fund, Macau SAR/ GR - 2024M762281/China Postdoctoral Science Foundation/ GR - GZC20250679/China Postdoctoral Fellowship Program of CPSF/ GR - 2025ZB029/Jiangsu Funding Program for Excellent Postdoctoral Talent/ GR - BK20250802/Natural Science Foundation of Jiangsu Province of China/ GR - SYW2024068/Suzhou Applied Basic Research (Healthcare) Science and Technology Innovation Program/ GR - Priority Academic Program Development of Jiangsu Higher Education Institutions/ GR - 111 Project/ PT - Journal Article DEP - 20261027 PL - Germany TA - Angew Chem Int Ed Engl JT - Angewandte Chemie (International ed. in English) JID - 0370543 SB - IM OTO - NOTNLM OT - DNA nanoswitch OT - bacteria OT - maltodextrin OT - melittin OT - urinary tract infections EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 11:52 PHST- 2026/08/28 00:00 [revised] PHST- 2026/01/10 00:00 [received] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:52 [entrez] AID - e6007035 [pii] AID - 10.1002/anie.6007035 [doi] PST - aheadofprint SO - Angew Chem Int Ed Engl. 2026 Oct 27:e6007035. doi: 10.1002/anie.6007035. PMID- 42944686 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2574-3805 (Electronic) IS - 2574-3805 (Linking) VI - 9 IP - 10 DP - 2026 Oct 1 TI - Operational Challenges During Public Health Emergencies in Safety-Net Hospitals. PG - e2640799 LID - 10.1001/jamanetworkopen.2026.40799 [doi] LID - e2640799 AB - IMPORTANCE: Safety-net hospitals (SNHs) serve as essential public health hubs, providing direct clinical care and critical social services to vulnerable populations, yet gaps persist in their ability to reliably deliver these essential services during disasters. OBJECTIVE: To identify common challenges faced by SNHs and actionable approaches described by participants to strengthen future emergency response capacity at these critical institutions. DESIGN, SETTING, AND PARTICIPANTS: For this qualitative study, from April through November 2025, key informant interviews were conducted with individuals across SNHs selected to represent a spectrum of SNH types. Thematic analysis of site-level case studies was used to identify cross-cutting and context-specific patterns across 5 domains: load balancing, staffing needs, supply chain, crisis standards of care (CSC), and health care coalitions (HCCs). MAIN OUTCOMES AND MEASURES: Stakeholder perspectives of pandemic preparedness, response, and recovery were evaluated to understand strategies to mitigate future health care disasters. RESULTS: Analysis of interviews with 69 individuals from 10 SNH sites identified systemic vulnerabilities across all 5 domains in SNHs. SNHs rarely succeeded in transferring critically ill patients without extensive staff time spent setting up the transfer. SNHs often experienced severe workforce disruptions from limited staff pools and nurse attrition, leaving them dependent on temporary staff at higher costs. Just-in-time personal protective equipment procurement and constricted manufacturing supply chains created supply crises. Few SNHs implemented full CSC, but many worked to find creative solutions to prevent more extensive changes in care standards. Opinions on written CSC were mixed, with some preferring structured frameworks and others preferring flexibility. Experiences with HCCs varied widely, with SNHs embedded in well-resourced HCCs reporting better coordination and support. CONCLUSIONS AND RELEVANCE: In this qualitative study of SNH preparedness and response, findings indicated that SNHs faced challenges with load balancing, supply chain, staffing, CSC, and HCCs. To address such challenges in the future, SNHs, regional coalitions, and the federal government should implement sustainable changes that can be rapidly brought to bear in an emergency. Potential solutions may have outsized benefit for SNHs; however, such solutions must be designed to be accessible to SNHs and feasible in these settings. FAU - Sell, Tara Kirk AU - Sell TK AD - Johns Hopkins Center for Health Security, Baltimore, Maryland. AD - Department of Environmental Health and Engineering, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland. FAU - Gregoire, Vanessa AU - Gregoire V AD - Johns Hopkins Center for Health Security, Baltimore, Maryland. FAU - Neufeld, Madisen S AU - Neufeld MS AD - Johns Hopkins Center for Health Security, Baltimore, Maryland. FAU - Adler, Rachel R AU - Adler RR AD - Center for Surgery and Public Health, Department of Surgery, Mass General Brigham, Boston, Massachusetts. AD - Now with Department of Medicine, University of Colorado Anschutz, Aurora. FAU - Gosselin, Geoffrey AU - Gosselin G AD - Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland. FAU - Pomer, Alysa AU - Pomer A AD - Center for Surgery and Public Health, Department of Surgery, Mass General Brigham, Boston, Massachusetts. FAU - Tial, Biak L AU - Tial BL AD - Johns Hopkins Center for Health Security, Baltimore, Maryland. AD - Department of Environmental Health and Engineering, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland. FAU - Weissman, Joel S AU - Weissman JS AD - Center for Surgery and Public Health, Department of Surgery, Mass General Brigham, Boston, Massachusetts. FAU - Goralnick, Eric AU - Goralnick E AD - Center for Surgery and Public Health, Department of Surgery, Mass General Brigham, Boston, Massachusetts. AD - Department of Emergency Medicine, Mass General Brigham, Harvard Medical School, Boston, Massachusetts. LA - eng PT - Journal Article DEP - 20261001 PL - United States TA - JAMA Netw Open JT - JAMA network open JID - 101729235 SB - IM CIN - doi: 10.1001/jamanetworkopen.2026.40744 MH - Humans MH - *Safety-net Providers/organization & administration MH - Qualitative Research MH - Public Health Infrastructure MH - *Public Health/methods MH - *Disaster Planning/organization & administration MH - *Emergencies MH - Pandemic Preparedness MH - COVID-19/epidemiology PMC - PMC13714659 COIS- Conflict of Interest Disclosures: Dr Sell reported directing the Department of Health and Human Services region 3 Public Health Preparedness and Response Center, which is funded through a cooperative agreement with the US Centers for Disease Control and Prevention, outside the submitted work. Mr Gosselin reported receiving personal fees from Rhode Island Department of Health State Laboratories for performing genetic sequencing for use in COVID-19 surveillance from to April 2022 to May 2024; being a contract worker designing infection prevention and control educational materials for staff across the entire health care ecosystem for the Rhode Island Department of Health from May 2024 to September 2025; and receiving personal fees from Beth Israel Lahey Health, a large hospital system in Massachusetts and New Hampshire since March 2026, for work as an Emergency Management Specialist outside the submitted work. Dr Goralnick reported serving as Secretary, Executive Office of Veterans Services, Commonwealth of Massachusetts outside the submitted work. No other disclosures were reported. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 11:33 PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:33 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 2855862 [pii] AID - zoi261103 [pii] AID - 10.1001/jamanetworkopen.2026.40799 [doi] PST - epublish SO - JAMA Netw Open. 2026 Oct 1;9(10):e2640799. doi: 10.1001/jamanetworkopen.2026.40799. PMID- 42944679 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 2531-0437 (Electronic) IS - 2531-0429 (Linking) VI - 32 IP - 1 DP - 2026 Dec 31 TI - A clinical-radiological model for predicting in-hospital mortality in acute exacerbation of COPD. PG - 2754553 LID - 10.1080/25310429.2026.2754553 [doi] AB - BACKGROUND: Quantitative computed tomography (QCT) has emerged as a promising tool for chronic obstructive pulmonary disease (COPD) phenotyping. However, its prognostic value in acute exacerbations of COPD (AECOPD) remains insufficiently explored, and existing risk stratification models have limited integration of imaging-derived metrics. This study aimed to develop and validate a prognostic model that integrates multidimensional clinical variables with QCT-derived metrics, and to compare its predictive performance with traditional clinical scores for in-hospital mortality in AECOPD. METHODS: In this retrospective cohort study, consecutive patients aged >/=40 years hospitalized with severe AECOPD were enrolled from September 2010 to October 2024, with collection of demographic characteristics, clinical features, laboratory indices, and chest CT imaging data. The cohort was randomly split into training and testing sets (7:3). Candidate predictors were selected using least absolute shrinkage and selection operator (LASSO) regression followed by multivariable logistic regression. Four classification models were developed and evaluated using 10-fold cross-validation. Discriminative performance was assessed by area under the receiver operating characteristic curve (AUC) and compared with conventional clinical scores. RESULTS: A total of 465 patients with AECOPD were included, of whom 47 (10.1%) died during hospitalization. Five independent predictors were identified: confusion, lymphocyte count <0.8 x 10(9)/L, chronic heart failure, white blood cell count >/=10 x 10(9)/L, and reduced pulmonary vascular fractal dimension. The logistic regression model demonstrated the best performance, achieving an AUC of 0.852 (95% CI, 0.738-0.965) in the testing set, significantly outperforming other models and established clinical scores. CONCLUSIONS: A predictive model integrating QCT-derived metrics with clinical features improved AECOPD in-hospital mortality prediction over traditional scores, supporting its utility for individualized risk stratification and management. FAU - Li, Yang AU - Li Y AD - Department of Respiratory and Critical Care Medicine, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China. AD - School of Medicine, University of the Chinese Academy of Sciences, Beijing, China. FAU - Wang, Xiaoyan AU - Wang X AD - Department of Respiratory and Critical Care Medicine, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China. FAU - Zhang, Min AU - Zhang M AD - Department of Radiology, Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China. FAU - Wang, Yuxia AU - Wang Y AD - Department of Respiratory and Critical Care Medicine, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China. FAU - Wang, Guoxuan AU - Wang G AD - Department of Radiology, Beijing Hospital, National Center of Gerontology; Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China. FAU - Hu, Yanlu AU - Hu Y AD - Department of Radiology, West China Hospital, Sichuan University, Chengdu, Sichuan, China. FAU - Wei, Yaping AU - Wei Y AD - College of Public Health, Shanghai University of Medicine and Health Sciences, Shanghai, China. FAU - Guo, Yanfei AU - Guo Y AUID- ORCID: 0000-0002-0437-3766 AD - Department of Respiratory and Critical Care Medicine, Beijing Hospital, National Center of Gerontology, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing, China. LA - eng PT - Journal Article DEP - 20261027 PL - United States TA - Pulmonology JT - Pulmonology JID - 101723786 SB - IM MH - Humans MH - *Pulmonary Disease, Chronic Obstructive/mortality/diagnostic imaging/physiopathology MH - *Hospital Mortality MH - Retrospective Studies MH - *Tomography, X-Ray Computed/methods MH - Aged MH - Female MH - Male MH - Prognosis MH - Disease Progression MH - Middle Aged MH - Predictive Value of Tests MH - Risk Assessment/methods MH - ROC Curve MH - Logistic Models OTO - NOTNLM OT - Acute exacerbation OT - chronic obstructive pulmonary disease OT - in-hospital mortality OT - logistic regression OT - quantitative computed tomography EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 11:33 PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:33 [entrez] AID - 10.1080/25310429.2026.2754553 [doi] PST - ppublish SO - Pulmonology. 2026 Dec 31;32(1):2754553. doi: 10.1080/25310429.2026.2754553. Epub 2026 Oct 27. PMID- 42944678 OWN - NLM STAT- Publisher LR - 20261029 IS - 2198-3844 (Electronic) IS - 2198-3844 (Linking) DP - 2026 Oct 27 TI - Surface-Selective Pt Enrichment for Stabilizing Ultrathin 2D PtCu Nanosheets Toward Durable Oxygen Reduction. LID - e78520 [pii] LID - 10.1002/advs.78520 [doi] AB - Ultrathin Pt-based 2D alloy nanosheets are promising electrocatalysts for the oxygen reduction reaction (ORR) due to their high surface-to-volume ratios and favorable facet-dependent activity. However, their thermodynamic instability and susceptibility to transition metal dissolution present critical barriers to their practical application. While surface Pt enrichment could mitigate such degradation, constructing Pt-rich surfaces on 2D nanosheets remains challenging, as conventional approaches often induce structural collapse or undesired thickening. Herein, we report a surface-selective Pt enrichment strategy via galvanic replacement to stabilize ultrathin PtCu nanosheets (PtCu NS/C) while preserving their unique 2D integrity. PtCu@Pt NS/C features a Pt-enriched outer region and an alloyed subsurface, which effectively suppresses Cu exposure while maintaining beneficial electronic interactions. PtCu@Pt NS/C delivers high ORR activity, outperforming commercial Pt/C. More importantly, PtCu@Pt NS/C exhibits remarkable durability, with 17.5% mass activity loss after 30 000 accelerated stress test cycles. Notably, in single-cell PEMFC configurations, PtCu@Pt NS/C demonstrates outstanding voltage stability with a minimal loss of 4 mV at 0.2 A cm(-2), whereas PtCu NS/C and Pt/C suffer much larger losses of 41 and 38 mV, respectively. This work demonstrates that preferential near-surface Pt enrichment effectively stabilizes PtCu nanosheets, offering a surface-engineering approach for improving the durability of ultrathin 2D alloy electrocatalysts. CI - (c) 2026 The Author(s). Advanced Science published by Wiley-VCH GmbH. FAU - Yang, HyunWoo J AU - Yang HJ AUID- ORCID: 0009-0001-3246-6548 AD - Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. FAU - Lee, SangJae AU - Lee S AD - Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. FAU - Lee, KwangHo AU - Lee K AUID- ORCID: 0000-0001-7512-8824 AD - Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. FAU - Chang, HyunWoo AU - Chang H AD - Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. FAU - Yang, HyunWoo T AU - Yang HT AUID- ORCID: 0009-0009-8231-7194 AD - Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. FAU - Shin, Dongwon AU - Shin D AUID- ORCID: 0009-0004-9295-3331 AD - Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. FAU - Kim, MinJun AU - Kim M AD - Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. FAU - Cho, EunAe AU - Cho E AUID- ORCID: 0000-0002-2871-6903 AD - Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea. LA - eng GR - Korea Institute of Energy Technology Evaluation and Planning (KETEP)/ GR - RS-2021-KP002516/Ministry of Climate, Energy and Environment/ GR - National Research Foundation of Korea (NRF)/ GR - RS-2024-00466554/Ministry of Science and ICT/ PT - Journal Article DEP - 20261027 PL - Germany TA - Adv Sci (Weinh) JT - Advanced science (Weinheim, Baden-Wurttemberg, Germany) JID - 101664569 SB - IM PMC - PMC13712395 OTO - NOTNLM OT - 2D nanosheets OT - galvanic replacement reaction OT - oxygen reduction reaction OT - shape-controlled catalysts OT - surface-selective Pt enrichment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 PMCR- 2026/10/27 CRDT- 2026/10/27 11:33 PHST- 2026/10/19 00:00 [revised] PHST- 2026/05/08 00:00 [received] PHST- 2026/10/22 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:33 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e78520 [pii] AID - ADVS78520 [pii] AID - 10.1002/advs.78520 [doi] PST - aheadofprint SO - Adv Sci (Weinh). 2026 Oct 27:e78520. doi: 10.1002/advs.78520. PMID- 42944670 OWN - NLM STAT- Publisher LR - 20261027 IS - 2213-1787 (Electronic) IS - 2213-1779 (Linking) DP - 2026 Oct 24 TI - Transcriptomics Identifies a Small Set of Circulating Biomarkers and Signaling Pathways Dysregulated in Hypertrophic Cardiomyopathy. PG - 103470 LID - S2213-1779(26)00577-9 [pii] LID - 10.1016/j.jchf.2026.103470 [doi] AB - BACKGROUND: It is sometimes challenging to distinguish hypertrophic cardiomyopathy (HCM) from other cardiomyopathies with left ventricular hypertrophy (LVH). At present, no circulating biomarkers to distinguish HCM have been established. OBJECTIVES: The authors aimed to identify plasma microRNA (miRNA) biomarkers unique to HCM. METHODS: In this multicenter case-control study, the authors performed comprehensive plasma transcriptomics profiling in cases with HCM and controls with hypertensive LVH, transthyretin amyloid cardiomyopathy (ATTR-CM), and aortic stenosis (AS). For each disease group, they chronologically split the patients into training and test sets. The authors identified miRNAs that were significantly (Benjamini-Hochberg-adjusted P < 0.05) upregulated or downregulated in HCM compared with hypertensive LVH. They made the same comparisons between HCM and ATTR-CM and between HCM and AS. They determined miRNAs that were consistently upregulated or downregulated in HCM throughout all 3 comparisons. Then, they selected miRNAs independently associated with HCM after adjusting for potential confounders. Using the selected miRNAs, the authors developed a logistic regression model to distinguish HCM in the training set. They calculated the area under the receiver-operating characteristic curve in the test set. RESULTS: The authors analyzed 2,656 miRNAs in patients with HCM (n = 294), hypertensive LVH (n = 321), ATTR-CM (n = 167), and AS (n = 38). They identified 3 miRNAs (miR-454-3p, miR-98-5p, and miR-5581-3p). The logistic regression model using the 3 miRNAs had an area under the receiver-operating characteristic curve of 0.95 (95% CI: 0.93-0.97) with a sensitivity of 0.95 and a specificity of 0.90 in the test set. CONCLUSIONS: Comprehensive plasma transcriptomics profiling identified a small set of circulating miRNAs to distinguish HCM from other cardiomyopathies with LVH. CI - Copyright (c) 2026 American College of Cardiology Foundation. Published by Elsevier Inc. All rights reserved. FAU - Kiyohara, Yuko AU - Kiyohara Y AD - Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center, New York, New York, USA. FAU - Akita, Keitaro AU - Akita K AD - Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center, New York, New York, USA. FAU - Fifer, Michael A AU - Fifer MA AD - Cardiology Division, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA. FAU - Tower-Rader, Albree AU - Tower-Rader A AD - Cardiology Division, Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA. FAU - Teruya, Sergio AU - Teruya S AD - Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center, New York, New York, USA. FAU - Ansari, Bilal AU - Ansari B AD - Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center, New York, New York, USA. FAU - Santos, Alfonsina M AU - Santos AM AD - Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center, New York, New York, USA. FAU - Maurer, Mathew S AU - Maurer MS AD - Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center, New York, New York, USA. FAU - Shimada, Yuichi J AU - Shimada YJ AD - Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center, New York, New York, USA. Electronic address: ys3053@cumc.columbia.edu. LA - eng PT - Journal Article DEP - 20261024 PL - United States TA - JACC Heart Fail JT - JACC. Heart failure JID - 101598241 SB - IM OTO - NOTNLM OT - aortic stenosis OT - biomarkers OT - hypertrophic cardiomyopathy OT - left ventricular hypertrophy OT - transthyretin amyloid cardiomyopathy COIS- Funding Support and Author Disclosures This work was supported by the National Institutes of Health (NIH) (R01 HL157216 and R01 HL168382), the American Heart Association (2 National Clinical and Population Research Awards, 1 Career Development Award, and 1 Transformational Project Award), the Korea Institute of Oriental Medicine (W25001), the Feldstein Medical Foundation, the Columbia University Irving Medical Center Precision Medicine Pilot Award, and the Columbia University Irving Medical Center Marjorie and Lewis Katz Cardiovascular Research Prize all to Dr Shimada. Dr Maurer has received grant support from the NIH (R01 HL139671 and R01 AG081582); and consulting income from Akcea, Alnylam, Eidos Therapeutics, Pfizer, Prothena, Novo Nordisk, and Intellia. Dr Shimada has received funding from Bristol Myers Squibb; and consulting income from Bristol Myers Squibb and Moderna, Japan. The researchers were independent from the funding organizations. All other authors have reported that they have no relationships relevant to the contents of this paper to disclose. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 11:31 PHST- 2026/02/17 00:00 [received] PHST- 2026/08/17 00:00 [revised] PHST- 2026/08/25 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:31 [entrez] AID - S2213-1779(26)00577-9 [pii] AID - 10.1016/j.jchf.2026.103470 [doi] PST - aheadofprint SO - JACC Heart Fail. 2026 Oct 24:103470. doi: 10.1016/j.jchf.2026.103470. PMID- 42944660 OWN - NLM STAT- Publisher LR - 20261027 IS - 2666-0849 (Electronic) IS - 2666-0849 (Linking) DP - 2026 Oct 26 TI - Salvage Transcatheter Mitral Valve-in-Valve With Aspiration Thrombectomy in a Young Adult With Repaired CHD. PG - 110863 LID - S2666-0849(26)04023-4 [pii] LID - 10.1016/j.jaccas.2026.110863 [doi] AB - BACKGROUND: Prosthetic mitral valve degeneration in adults with repaired congenital heart disease (CHD) can produce rapid hemodynamic collapse and is challenging when surgery and mechanical circulatory support (MCS) are unavailable. CASE SUMMARY: A 20-year-old woman with congenital mitral stenosis and multiple ventricular septal defects initially palliated as a single ventricle, then converted to biventricular circulation, with prior mechanical and bioprosthetic mitral valve replacements (the latter for menorrhagia, outside our care), presented in cardiogenic shock with prosthetic mitral stenosis and acute left atrial thrombus. Hypoplastic peripheral vessels precluded MCS. Salvage valve-in-valve was performed with bilateral cerebral protection, FlowTriever thrombectomy (Inari Medical), and a 26-mm SAPIEN 3 valve (Edwards Lifesciences). DISCUSSION: Transcatheter mitral valve-in-valve can be lifesaving in adults with repaired CHD when intracardiac thrombus is present, and MCS is unavailable. TAKE-HOME MESSAGES: In highly selected, life-threatening presentations without alternatives, salvage transcatheter mitral intervention may be considered despite intracardiac thrombus. Peripheral vascular anatomy must be evaluated preprocedurally in complex CHD. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Ali, Rubiya AU - Ali R AD - Memorial Healthcare System, Pembroke Pines, Florida, USA. Electronic address: ruali@mhs.net. FAU - Naeem, Muniba AU - Naeem M AD - Memorial Healthcare System, Pembroke Pines, Florida, USA. FAU - Tagle Tejeda, Alfredo Perez AU - Tagle Tejeda AP AD - Memorial Healthcare System, Pembroke Pines, Florida, USA. FAU - Pahuja, Karan AU - Pahuja K AD - Memorial Healthcare System, Pembroke Pines, Florida, USA. FAU - Roth, Todd AU - Roth T AD - Memorial Healthcare System, Pembroke Pines, Florida, USA. FAU - Boe, Brian AU - Boe B AD - Memorial Healthcare System, Pembroke Pines, Florida, USA. FAU - Guyon, Peter W AU - Guyon PW AD - Memorial Healthcare System, Pembroke Pines, Florida, USA. FAU - Ho, Annie H AU - Ho AH AD - Memorial Healthcare System, Pembroke Pines, Florida, USA. FAU - Khalili, Houman AU - Khalili H AD - Memorial Healthcare System, Pembroke Pines, Florida, USA. FAU - Saef, Joshua AU - Saef J AD - Memorial Healthcare System, Pembroke Pines, Florida, USA. LA - eng PT - Case Reports PT - Journal Article DEP - 20261026 PL - Netherlands TA - JACC Case Rep JT - JACC. Case reports JID - 101757292 OTO - NOTNLM OT - aspiration thrombectomy OT - cardiogenic shock OT - congenital heart disease OT - intracardiac thrombus OT - salvage intervention OT - transcatheter mitral valve-in-valve COIS- Funding Support and Author Disclosures The authors have reported that they have no relationships relevant to the contents of this paper to disclose. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 11:31 PHST- 2026/07/13 00:00 [received] PHST- 2026/09/07 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:31 [entrez] AID - S2666-0849(26)04023-4 [pii] AID - 10.1016/j.jaccas.2026.110863 [doi] PST - aheadofprint SO - JACC Case Rep. 2026 Oct 26:110863. doi: 10.1016/j.jaccas.2026.110863. PMID- 42944659 OWN - NLM STAT- Publisher LR - 20261027 IS - 2666-0849 (Electronic) IS - 2666-0849 (Linking) DP - 2026 Oct 26 TI - Catheter-Induced Type B Aortic Dissection After TAVI: Delayed Adverse Remodeling. PG - 110845 LID - S2666-0849(26)04005-2 [pii] LID - 10.1016/j.jaccas.2026.110845 [doi] AB - BACKGROUND: Catheter-induced type B aortic dissection is a rare complication of transcatheter aortic valve implantation with uncertain long-term behavior. CASE SUMMARY: An 85-year-old woman developed a focal aortic isthmus dissection during transfemoral transcatheter aortic valve implantation. Initial computed tomography angiography (CTA) showed no malperfusion or rupture; conservative management and serial CTA surveillance were selected. Despite persistent clinical stability, follow-up CTA demonstrated progressive focal outpouching and pseudoaneurysmal remodeling. At 17 months, elective thoracic endovascular aortic repair achieved complete exclusion of the lesion. DISCUSSION: Catheter-induced dissection may represent a mechanistically distinct entity from spontaneous type B aortic dissection, limiting direct extrapolation of conventional management pathways. Morphological progression, rather than symptoms or absolute aortic diameter, prompted intervention. TAKE-HOME MESSAGES: Clinical stability does not establish aortic wall healing. Long-term serial CTA can identify silent adverse remodeling and guide elective thoracic endovascular aortic repair in selected patients. CI - Copyright (c) 2026 The Authors. Published by Elsevier Inc. All rights reserved. FAU - Bargagna, Federico AU - Bargagna F AD - Cardiothoracic and Vascular Department, Azienda Ospedaliero-Universitaria Pisana, Pisa, Italy. FAU - Casula, Marta AU - Casula M AD - Clinical Experimental Cardiology, Clinical and Interventional Cardiology, University of Sassari, Sassari, Italy. Electronic address: casulamarta681@gmail.com. FAU - Mori, Francesco AU - Mori F AD - Cardiothoracic and Vascular Department, Azienda Ospedaliero-Universitaria Pisana, Pisa, Italy. FAU - Ravani, Marcello AU - Ravani M AD - Department of Cardiology, G. Pasquinucci Heart Hospital, Fondazione Monasterio, Massa, Italy. FAU - Al Jabri, Anees AU - Al Jabri A AD - Department of Cardiology, G. Pasquinucci Heart Hospital, Fondazione Monasterio, Massa, Italy. FAU - Berti, Sergio AU - Berti S AD - Department of Cardiology, G. Pasquinucci Heart Hospital, Fondazione Monasterio, Massa, Italy. FAU - Rizza, Antonio AU - Rizza A AD - Department of Cardiology, G. Pasquinucci Heart Hospital, Fondazione Monasterio, Massa, Italy. LA - eng PT - Case Reports PT - Journal Article DEP - 20261026 PL - Netherlands TA - JACC Case Rep JT - JACC. Case reports JID - 101757292 OTO - NOTNLM OT - computed tomography angiography OT - pseudoaneurysm OT - serial imaging OT - structural heart intervention OT - thoracic endovascular repair OT - vascular surveillance COIS- Funding Support and Author Disclosures The authors have reported that they have no relationships relevant to the contents of this paper to disclose. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 11:31 PHST- 2026/06/03 00:00 [received] PHST- 2026/08/12 00:00 [revised] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:31 [entrez] AID - S2666-0849(26)04005-2 [pii] AID - 10.1016/j.jaccas.2026.110845 [doi] PST - aheadofprint SO - JACC Case Rep. 2026 Oct 26:110845. doi: 10.1016/j.jaccas.2026.110845. PMID- 42944648 OWN - NLM STAT- Publisher LR - 20261027 IS - 1469-0705 (Electronic) IS - 0960-7692 (Linking) DP - 2026 Oct 27 TI - Long-term gynecological symptoms and ultrasound and reproductive outcomes after monofilament vs multifilament uterine closure at Cesarean delivery: secondary analysis of randomized controlled trial. LID - 10.1002/uog.70365 [doi] AB - OBJECTIVE: To evaluate long-term gynecological symptoms and reproductive and ultrasound outcomes among women originally enrolled in a randomized controlled trial (RCT) comparing monofilament and multifilament sutures for uterine closure after Cesarean delivery. METHODS: This was a secondary analysis of a RCT including women who underwent Cesarean delivery, who were originally assigned to monofilament or multifilament absorbable sutures for uterine closure. In this secondary analysis, participants were contacted 4-6 years after delivery and invited to a follow-up evaluation, comprising a clinical interview and a voluntary transvaginal ultrasound examination. The primary outcome was the presence of postmenstrual spotting at long-term follow-up. Secondary outcomes were other gynecological symptoms, reproductive outcomes and ultrasound outcomes. Analyses were performed according to the original randomized allocation. Effect estimates are reported as relative risk (RR) with 95% CI. RESULTS: Of the 300 women included in the original RCT, 117 (39.0%) were included in the long-term follow-up analysis, comprising 51 in the monofilament group and 66 in the multifilament group. There was no significant difference in the primary outcome of postmenstrual spotting between the monofilament and multifilament groups (23.5% vs 22.7%; RR, 1.04 (95% CI, 0.53-2.01)). Similarly, no significant differences were observed between groups for other gynecological symptoms. Ultrasound data were available for 21 women, of whom 13 had a Cesarean-scar defect. The overall prevalence of Cesarean-scar defects was similar between groups, although defects >/= 4 mm were more frequent in the multifilament group (RR, 0.42 (95% CI, 0.18-0.97); P = 0.04). Most reproductive outcomes, including infertility and pregnancy outcomes among women who conceived, were comparable between groups. However, subsequent pregnancy occurred more frequently in the monofilament group (29.4% vs 12.1%; RR, 2.43 (95% CI, 1.12-5.27)). CONCLUSIONS: In this long-term follow-up of a RCT, monofilament and multifilament sutures for uterine closure were associated with similar long-term gynecological symptoms. Differences observed in selected ultrasound and reproductive outcomes should be interpreted cautiously because of the limited sample size and exploratory nature of these analyses. Further studies are needed to better evaluate the potential impact of suture material on long-term reproductive outcomes and sonographic Cesarean-scar characteristics. (c) 2026 The Author(s). Ultrasound in Obstetrics & Gynecology published by John Wiley & Sons Ltd on behalf of International Society of Ultrasound in Obstetrics and Gynecology. CI - (c) 2026 The Author(s). Ultrasound in Obstetrics & Gynecology published by John Wiley & Sons Ltd on behalf of International Society of Ultrasound in Obstetrics and Gynecology. FAU - Ziello, C AU - Ziello C AUID- ORCID: 0009-0000-5786-9485 AD - Department of Public Health, School of Medicine, University of Naples Federico II, Naples, Italy. FAU - Pacilio, A M AU - Pacilio AM AUID- ORCID: 0009-0009-7497-8961 AD - Department of Public Health, School of Medicine, University of Naples Federico II, Naples, Italy. FAU - Niccolini, L AU - Niccolini L AUID- ORCID: 0009-0004-2232-2702 AD - Department of Public Health, School of Medicine, University of Naples Federico II, Naples, Italy. FAU - Mola, S AU - Mola S AUID- ORCID: 0009-0007-8313-1143 AD - Department of Public Health, School of Medicine, University of Naples Federico II, Naples, Italy. FAU - Casella, C AU - Casella C AD - Department of Advanced Biomedical Sciences, University of Naples Federico II, Naples, Italy. FAU - Silvestri, A AU - Silvestri A AUID- ORCID: 0009-0009-9166-1325 AD - Department of Public Health, School of Medicine, University of Naples Federico II, Naples, Italy. FAU - Falco, L AU - Falco L AUID- ORCID: 0009-0003-6444-2828 AD - Department of Public Health, School of Medicine, University of Naples Federico II, Naples, Italy. FAU - Di Spiezio Sardo, A AU - Di Spiezio Sardo A AD - Department of Public Health, School of Medicine, University of Naples Federico II, Naples, Italy. FAU - Saccone, G AU - Saccone G AUID- ORCID: 0000-0003-0078-2113 AD - Department of Neuroscience, Reproductive Science and Dentistry, School of Medicine, University of Naples Federico II, Naples, Italy. CN - Collaborators LA - eng PT - Journal Article DEP - 20261027 PL - England TA - Ultrasound Obstet Gynecol JT - Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology JID - 9108340 SB - IM OTO - NOTNLM OT - Cesarean delivery OT - Cesarean-scar defect OT - isthmocele OT - postmenstrual spotting OT - suture material OT - uterine closure FIR - Bello, A IR - Bello A FIR - Di Napoli, F IR - Di Napoli F FIR - Murolo, C IR - Murolo C FIR - Merone, A M IR - Merone AM EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 11:23 PHST- 2026/06/19 00:00 [revised] PHST- 2026/04/20 00:00 [received] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:23 [entrez] AID - 10.1002/uog.70365 [doi] PST - aheadofprint SO - Ultrasound Obstet Gynecol. 2026 Oct 27. doi: 10.1002/uog.70365. PMID- 42944646 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1755-0998 (Electronic) IS - 1755-098X (Print) IS - 1755-098X (Linking) VI - 26 IP - 8 DP - 2026 Nov TI - Active Filtration of Airborne eDNA for Vertebrate Surveys in Tropical Caves. LID - e70218 [pii] LID - 10.1111/1755-0998.70218 [doi] AB - Hidden from sunlight, caves are remarkable subterraneous ecosystems that host a wide range of animals, including unique species. Conventional cave biodiversity surveys can be physically challenging, disturb sensitive vertebrate communities, or require animal collection. Here we evaluate the use of metabarcoding of airborne environmental DNA (eDNA) as a non-invasive method for surveying cave vertebrates. We collected 55 samples by filtering air for 24 h at three light zones (outside, and in twilight and dark zones inside the cave) in three tropical caves in Amazonian Brazil. Using two metabarcoding primer sets targeting mammal and vertebrate DNA, we detected 123 wild vertebrate taxa encompassing mammals, birds, amphibians, and a reptile, of which 74 were identified to genus level. Genus richness in the twilight zone was significantly higher than outside the caves. Vertebrate diversity was also highest in the twilight zone, and community composition differed significantly between light zones and caves. Mammals and amphibians were mostly detected in the twilight and dark zones, whereas birds were more frequently detected outside the cave and in the twilight zone. While preliminary, our results show that caves with smaller entrances yielded more homogeneous communities in the twilight and dark zones. We detected eight of the 13 bat species and one of the four amphibian species recorded in previous surveys, while also detecting taxa not previously recorded in the caves, demonstrating complementarity with conventional surveys. As airborne eDNA requires minimal human presence in cave interiors, it reduces disturbance to sensitive fauna, lowers safety risks to surveyors, and offers a scalable method to complement conventional cave biodiversity surveys in tropical regions. CI - (c) 2026 The Author(s). Molecular Ecology Resources published by John Wiley & Sons Ltd. FAU - Lynggaard, Christina AU - Lynggaard C AUID- ORCID: 0000-0003-4451-6642 AD - Faculty of Health and Medical Sciences, Globe Institute, University of Copenhagen, Copenhagen, Denmark. FAU - Bodawatta, Kasun Harshana AU - Bodawatta KH AUID- ORCID: 0000-0002-6095-9059 AD - Faculty of Health and Medical Sciences, Globe Institute, University of Copenhagen, Copenhagen, Denmark. FAU - de Fraga, Rafael AU - de Fraga R AUID- ORCID: 0000-0002-9900-4276 AD - Instituto Tecnologico Vale, Belem, Brazil. FAU - Martins, Vitoria Catarina Cardoso AU - Martins VCC AUID- ORCID: 0000-0002-8666-6326 AD - Instituto Tecnologico Vale, Belem, Brazil. FAU - Aleixo, Alexandre AU - Aleixo A AUID- ORCID: 0000-0002-7816-9725 AD - Instituto Tecnologico Vale, Belem, Brazil. FAU - Tavares, Valeria da C AU - Tavares VDC AD - Instituto Tecnologico Vale, Belem, Brazil. AD - Pos-Graduacao em Biodiversidade e Evolucao, Museu Paraense Emilio Goeldi, Belem, Brazil. AD - Pos-Graduacao em Biologia (Zoologia), PPGCB, Departamento de Sistematica e Ecologia, Universidade Federal da Paraiba (UFPB), Joao Pessoa, Brazil. FAU - Trevelin, Leonardo C AU - Trevelin LC AUID- ORCID: 0000-0002-1041-6902 AD - Instituto Tecnologico Vale, Belem, Brazil. AD - Pos-Graduacao em Biodiversidade e Evolucao, Museu Paraense Emilio Goeldi, Belem, Brazil. FAU - Gastauer, Markus AU - Gastauer M AUID- ORCID: 0000-0002-9599-0902 AD - Instituto Tecnologico Vale, Belem, Brazil. FAU - Bohmann, Kristine AU - Bohmann K AUID- ORCID: 0000-0001-7907-064X AD - Faculty of Health and Medical Sciences, Globe Institute, University of Copenhagen, Copenhagen, Denmark. LA - eng GR - CF21-0411/Carlsbergfondet/ GR - R100603.NNL.04/Instituto Tecnologico Vale/ GR - R100603.CR.04/Instituto Tecnologico Vale/ GR - VIL41390/Villum Fonden/ GR - 309243/2023-8/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ GR - 313429/2025-1/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ GR - 310865/2022-0/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ GR - 441201/2023-7/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ PT - Evaluation Study PT - Journal Article PL - England TA - Mol Ecol Resour JT - Molecular ecology resources JID - 101465604 RN - 0 (DNA, Environmental) RN - 0 (Extrachromosomal DNA) SB - IM MH - Animals MH - *Caves MH - Brazil MH - *Vertebrates/classification/genetics MH - *DNA, Environmental/genetics/isolation & purification MH - *DNA Barcoding, Taxonomic/methods MH - Biodiversity MH - Extrachromosomal DNA MH - *Filtration/methods MH - Tropical Climate MH - *Metagenomics/methods PMC - PMC13712314 OTO - NOTNLM OT - DNA metabarcoding OT - air OT - airborne eDNA OT - caves OT - tropics OT - vertebrates COIS- The authors declare the following financial relationships that could be perceived as potential competing interests: Alexandre Aleixo, Markus Gastauer, Valeria da C. Tavares, Leonardo Carreira Trevelin, and Rafael de Fraga have received financial support from Vale, a major mining company with operations that partially overlap the study area. However, Vale had no role in the study design, the collection, analysis or interpretation of data, the writing of the manuscript, or the decision to submit the work for publication. The other authors declare no known competing interests. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 11:23 PHST- 2026/10/02 00:00 [revised] PHST- 2026/07/18 00:00 [received] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:23 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e70218 [pii] AID - MEN70218 [pii] AID - 10.1111/1755-0998.70218 [doi] PST - ppublish SO - Mol Ecol Resour. 2026 Nov;26(8):e70218. doi: 10.1111/1755-0998.70218. PMID- 42944641 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1552-4965 (Electronic) IS - 1549-3296 (Linking) VI - 114 IP - 11 DP - 2026 Nov TI - Hydrocalumite-Like Material Induces Early Osteogenic Priming and ECM Remodeling Without Effective Mineralization in Preosteoblasts. LID - e70178 [pii] LID - 10.1002/jbm.a.70178 [doi] AB - Hydrocalumite-like phases, belonging to the layered double hydroxide family, are potential biomaterials for bone repair; however, their cellular effects remain incompletely understood. Here, we evaluated a hydrocalumite-like material of composition [Ca(2)Al(OH)(6)]Cl.nH(2)O (abbreviated Ca(2)Al-Cl) in preosteoblastic MC3T3-E1 cells, assessing viability, adhesion/signaling, osteogenic differentiation, extracellular matrix (ECM) remodeling, and cytokine profiles. Across a 0.002-0.2 mg mL(-1) range of Ca(2)Al-Cl, preserved cell viability and membrane integrity were observed, with the highest concentration (0.2 mg mL(-1)) eliciting the largest increase in metabolic activity (~270% of control), with a progressive reduction at lower concentrations; quantitative Crystal Violet staining showed increased total attachment at 0.2 and 0.02 mg mL(-1), while early adhesion transcripts (Integrin-beta1, FAK, Src, PP2A, Cofilin) decreased. Cell-cycle readouts revealed increased p21 and decreased CDK6, with CDK2/4 levels remaining unchanged. During 14-day differentiation, Osteocalcin increased, and ALP staining revealed more foci, yet Alpl, Runx2, and Osterix/Sp7 decreased, and Alizarin Red showed no visible mineral increase; OPG/RANKL were not modulated, thus the regulatory axis of bone resorption was not altered. The collagen content, as determined by Picrosirius staining, matched that of the Control, whereas the osteogenic medium was lower. Gelatin zymography indicated relatively higher MMP-2 (pro/intermediate/active) and lower MMP-9 under Ca(2)Al-Cl. Cytokine profiling revealed increased levels of IL-6 and IL-33, accompanied by reduced IL-1/IL-1beta and IL-10, and unchanged TNF. Collectively, Ca(2)Al-Cl induces an ECM-centric, early osteogenic priming phenotype characterized by selective remodeling (MMP-2-biased) and cytokine modulation without late mineral accrual under these conditions. These findings position Ca(2)Al-Cl as a potential modulator of the early bone regenerative microenvironment, a property that could be harnessed for applications where controlled matrix organization is prioritized over rapid mineralization. However, further studies quantifying ion release dynamics and utilizing 3D/co-culture models are essential to elucidate the causal mechanisms and translate this priming effect into a functional regenerative outcome. CI - (c) 2026 The Author(s). Journal of Biomedical Materials Research Part A published by Wiley Periodicals LLC. FAU - da Costa Fernandes, C J AU - da Costa Fernandes CJ AD - Laboratory of Bioassays and Cellular Dynamics, Department of Chemistry and Biochemistry, Institute of Biosciences, Sao Paulo State University (UNESP) Botucatu Campus, Sao Paulo, Brazil. AD - Department of Biophysics and Pharmacology, Institute of Biosciences, Sao Paulo State University (UNESP) Botucatu Campus, Sao Paulo, Brazil. FAU - de Almeida, G S AU - de Almeida GS AUID- ORCID: 0000-0002-0208-0441 AD - Laboratory of Bioassays and Cellular Dynamics, Department of Chemistry and Biochemistry, Institute of Biosciences, Sao Paulo State University (UNESP) Botucatu Campus, Sao Paulo, Brazil. FAU - Figueiredo, M Pires AU - Figueiredo MP AUID- ORCID: 0000-0002-9389-2057 AD - Laboratory for Biomaterials and Bioengineering, Canada Research Chair Tier I for the Innovation in Surgery, Department of Min-Met-Materials Engineering and Regenerative Medicine, CHU de Quebec Research Center, Laval University, Quebec City, Quebec, Canada. FAU - Constantino, V R L AU - Constantino VRL AUID- ORCID: 0000-0001-9276-7329 AD - Department of Fundamental Chemistry, Institute of Chemistry, University of Sao Paulo (USP), Sao Paulo, Brazil. FAU - Zambuzzi, W F AU - Zambuzzi WF AUID- ORCID: 0000-0002-4149-5965 AD - Laboratory of Bioassays and Cellular Dynamics, Department of Chemistry and Biochemistry, Institute of Biosciences, Sao Paulo State University (UNESP) Botucatu Campus, Sao Paulo, Brazil. LA - eng GR - 2016/13862-9/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/ GR - 2014/22689-3/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/ GR - 2011/50318-1/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/ GR - Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/ GR - Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ PT - Journal Article PL - United States TA - J Biomed Mater Res A JT - Journal of biomedical materials research. Part A JID - 101234237 RN - 5QB0T2IUN0 (Aluminum Hydroxide) RN - 0 (Cytokines) SB - IM MH - Animals MH - Mice MH - *Osteoblasts/cytology/metabolism/drug effects MH - *Extracellular Matrix/metabolism/drug effects MH - *Osteogenesis/drug effects MH - Cell Differentiation/drug effects MH - *Calcification, Physiologic/drug effects MH - Cell Line MH - *Aluminum Hydroxide/pharmacology/chemistry MH - Cell Adhesion/drug effects MH - Cell Survival/drug effects MH - Cytokines/metabolism OTO - NOTNLM OT - MC3T3-E1 OT - extracellular matrix remodeling OT - hydrocalumite OT - interleukin-6 OT - layered double hydroxides OT - matrix metalloproteinase-2 (MMP-2) OT - osteogenic differentiation EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 11:23 PHST- 2026/09/26 00:00 [revised] PHST- 2026/03/20 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:23 [entrez] AID - e70178 [pii] AID - 10.1002/jbm.a.70178 [doi] PST - ppublish SO - J Biomed Mater Res A. 2026 Nov;114(11):e70178. doi: 10.1002/jbm.a.70178. PMID- 42944637 OWN - NLM STAT- Publisher LR - 20261027 IS - 1521-3773 (Electronic) IS - 1433-7851 (Linking) DP - 2026 Oct 27 TI - Harnessing the Hard-Soft Acid-Base Principle in Sulfur-Dense Flexible Covalent Organic Frameworks for Targeted Gold Sequestration. LID - e8010323 [pii] LID - 10.1002/anie.8010323 [doi] AB - Efficient gold (Au) recovery from e-waste and industrial effluents is crucial for sustainable resource management, yet building crystalline covalent organic frameworks (COFs) with dense sulfur soft sites under Hard-Soft Acid-Base (HSAB) guidance is severely hindered by the intrinsic rotational freedom of thioether linkages, which typically precipitates amorphous networks. Herein, we report the rational synthesis of sulfur-bridged flexible COFs by tailoring solvothermal kinetics to enable the construction of crystalline topological framework isomers (sql and kgm nets) from an identical tetracyclic C-S bond-enriched monomer. The kgm framework was further functionalized with pendant methyl sulfide groups, generating abundant soft binding sites. Consequently, the resulting electron-rich framework delivers an exceptional Au uptake capacity of 6420 mg g(-1), ranking among the highest values reported to date for COFs under dark conditions, alongside rapid uptake kinetics and excellent selectivity. Combined spectroscopic and theoretical investigations reveal that sulfur sites play a central role in Au capture by providing abundant active sites, facilitating the coordination of Au species, and promoting electron transfer, thereby favoring the reduction of Au(III) to Au(0). This paradigm underscores the potency of the HSAB principle as a robust blueprint for designing high-performance adsorbents aimed at the sustainable precious metal recovery. CI - (c) 2026 Wiley-VCH GmbH. FAU - Ma, Yuxin AU - Ma Y AD - School of Carbon Neutrality Future Technology, Sichuan University, Chengdu, China. FAU - Ma, Liang AU - Ma L AD - School of Carbon Neutrality Future Technology, Sichuan University, Chengdu, China. FAU - Zou, Li-An AU - Zou LA AD - School of Resources and Environment Engineering, East China University of Science and Technology, Shanghai, China. FAU - Jin, Yucheng AU - Jin Y AUID- ORCID: 0000-0002-7008-4938 AD - School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China. FAU - Chen, Liyao AU - Chen L AUID- ORCID: 0009-0005-9788-6331 AD - School of Carbon Neutrality Future Technology, Sichuan University, Chengdu, China. FAU - Tian, Chengcheng AU - Tian C AUID- ORCID: 0000-0001-8952-5513 AD - School of Resources and Environment Engineering, East China University of Science and Technology, Shanghai, China. FAU - Zhou, Xiaohou AU - Zhou X AUID- ORCID: 0000-0001-5128-1082 AD - School of Carbon Neutrality Future Technology, Sichuan University, Chengdu, China. FAU - Xiang, Yuan AU - Xiang Y AUID- ORCID: 0000-0002-7231-7045 AD - School of Carbon Neutrality Future Technology, Sichuan University, Chengdu, China. FAU - Wang, Xia AU - Wang X AUID- ORCID: 0000-0002-8962-2724 AD - Max Planck Institute for Chemical Physics of Solids, Dresden, Germany. FAU - Wu, Jiwei AU - Wu J AUID- ORCID: 0000-0003-0136-604X AD - School of Carbon Neutrality Future Technology, Sichuan University, Chengdu, China. FAU - Li, Xiaodong AU - Li X AUID- ORCID: 0000-0002-9628-329X AD - Key Laboratory of Precision and Intelligent Chemistry, University of Science and Technology of China, Hefei, Anhui, China. FAU - Jiang, Xia AU - Jiang X AUID- ORCID: 0009-0006-6074-4863 AD - School of Carbon Neutrality Future Technology, Sichuan University, Chengdu, China. FAU - Zhu, Xiang AU - Zhu X AUID- ORCID: 0000-0002-3973-4998 AD - School of Carbon Neutrality Future Technology, Sichuan University, Chengdu, China. LA - eng GR - 22578296/National Natural Science Foundation of China/ GR - 52670139/National Natural Science Foundation of China/ GR - 2025YFHZ0177/Sichuan Science and Technology Program/ GR - 25H0291/Nantong Aichen Environmental Protection Technology Co., Ltd./ GR - BWLCF202408/Low-carbon Metallurgy Innovation Fund Project/ GR - 2023ZKZD41/Low-carbon Metallurgy Innovation Fund Project/ PT - Journal Article DEP - 20261027 PL - Germany TA - Angew Chem Int Ed Engl JT - Angewandte Chemie (International ed. in English) JID - 0370543 SB - IM OTO - NOTNLM OT - Au sequestration OT - densely populated soft binding sites OT - hard-soft acid-base principle OT - sulfur-bridged flexible COFs OT - topology switching EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 11:23 PHST- 2026/10/13 00:00 [revised] PHST- 2026/09/28 00:00 [received] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:23 [entrez] AID - e8010323 [pii] AID - 10.1002/anie.8010323 [doi] PST - aheadofprint SO - Angew Chem Int Ed Engl. 2026 Oct 27:e8010323. doi: 10.1002/anie.8010323. PMID- 42944627 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 1929-073X (Print) IS - 1929-073X (Electronic) IS - 1929-073X (Linking) VI - 15 DP - 2026 Oct 27 TI - Impact of Elevated Blood Pressure Targets on Splanchnic Ischemia and Mortality in Septic Resuscitation: Systematic Review. PG - e98772 LID - 10.2196/98772 [doi] LID - e98772 AB - BACKGROUND: Sepsis causes deaths worldwide each day, with hemodynamic resuscitation being central to management. Guidelines recommend a 65-mm Hg mean arterial pressure (MAP) target, yet the evidence for elevated targets remains fragmented across mortality, renal, and splanchnic outcomes. OBJECTIVE: This review aims to comprehensively appraise the impact of elevated MAP targets on splanchnic ischemia, mortality, and organ-specific outcomes in septic resuscitation. METHODS: Following the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) 2020 guidelines, we systematically searched PubMed, the Cochrane Library, and Google Scholar for studies comparing MAP targets in septic shock. Risk of bias was assessed using the Cochrane Risk of Bias 2 (RoB 2; randomized controlled trials [RCTs]) and the Newcastle-Ottawa Scale (nonrandomized studies). The findings were synthesized narratively according to the Synthesis Without Meta-Analysis guidelines, with random-effects meta-analysis for mortality outcomes. Evidence certainty was graded using the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) framework. RESULTS: From 3254 records, 11 studies (2895 patients; 4 RCTs, 7 nonrandomized) met the inclusion criteria. Pooled 90-day mortality across 3 trials showed a nonsignificant trend toward higher mortality with elevated MAP targets (320/720, 44.4% vs 280/722, 38.8%; OR 1.31, 95% CI 0.97-1.77). Pooled 28- or 30-day mortality showed a similar nonsignificant trend (OR 1.25, 95% CI 0.98-1.61), which was robust to sensitivity analysis. Narratively, mortality findings across nonpooled observational studies were inconsistent; some linked low MAP to higher death risk, and others found no significant association. Renal outcomes were inconsistent, with some studies showing reduced renal replacement therapy or improved acute kidney injury recovery at higher targets, particularly in hypertensive or cirrhotic subgroups. Elevated MAP targets consistently increased vasopressor exposure and were associated with more arrhythmias and fewer organ support-free days. No study reported splanchnic ischemia as a discrete outcome; mesenteric ischemia rates were low and similar between arms. Overall certainty of evidence was very low. CONCLUSIONS: Higher MAP targets show no clear mortality or splanchnic perfusion benefit and may increase overall harm, though select subgroups, chronic hypertension, and cirrhosis, may benefit, supporting individualized, risk-stratified MAP targeting over uniformly elevated-target strategies. CI - (c) Paulina Cisneros, John Fredy Ortiz Caviedes, Lina Maria Pineros Ladino, Natalia Quintero, Valeria Garcia Onate Cervera, Juan David Urrea Ospina, Erika Patricia Cerna Alcantar. Originally published in the Interactive Journal of Medical Research (https://www.i-jmr.org/). FAU - Cisneros, Paulina AU - Cisneros P AUID- ORCID: 0000-0002-4856-3852 AD - Cardiology Department, Guthrie Forssmann Cardiovascular Institute, Hospital Enrique Garces, Chilibulo S/N y Enrique Garces, Quito, Ecuador, 593 0999009697. FAU - Ortiz Caviedes, John Fredy AU - Ortiz Caviedes JF AUID- ORCID: 0000-0003-0664-3130 AD - Internal Medicine Department, Hospital San Rafael, Bogota, Colombia. FAU - Pineros Ladino, Lina Maria AU - Pineros Ladino LM AUID- ORCID: 0009-0008-4129-454x AD - Medicine Department, Preventivamente Salud IPS, Medellin, Colombia. FAU - Quintero, Natalia AU - Quintero N AUID- ORCID: 0009-0007-6214-5976 AD - Clinical Toxicology Department, Fundacion Universitaria de Ciencias de la Salud FUCS, Bogota, Colombia. FAU - Onate Cervera, Valeria Garcia AU - Onate Cervera VG AUID- ORCID: 0009-0008-4289-5216 AD - Medical Department, Escuela Medico Militar, Ciudad de Mexico, Mexico. FAU - Urrea Ospina, Juan David AU - Urrea Ospina JD AUID- ORCID: 0009-0000-7708-3287 AD - Medical Department, Universidad Libre de Colombia, Cali, Colombia. FAU - Cerna Alcantar, Erika Patricia AU - Cerna Alcantar EP AUID- ORCID: 0009-0002-8997-441X AD - Internal Medicine Department, Mexican Social Security Institute, San Luis Potosi, Mexico. LA - eng PT - Journal Article PT - Review DEP - 20261027 PL - Canada TA - Interact J Med Res JT - Interactive journal of medical research JID - 101598421 PMC - PMC13712110 OTO - NOTNLM OT - acute kidney injury OT - hemodynamic resuscitation OT - mean arterial pressure OT - septic shock OT - splanchnic perfusion OT - vasopressor therapy COIS- Conflicts of Interest: None declared. EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 11:13 PHST- 2026/04/18 00:00 [received] PHST- 2026/08/19 00:00 [revised] PHST- 2026/08/20 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:13 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - v15i1e98772 [pii] AID - 98772 [pii] AID - 10.2196/98772 [doi] PST - epublish SO - Interact J Med Res. 2026 Oct 27;15:e98772. doi: 10.2196/98772. PMID- 42944624 OWN - NLM STAT- Publisher LR - 20261027 IS - 1521-3773 (Electronic) IS - 1433-7851 (Linking) DP - 2026 Oct 27 TI - Tetraxene: Serendipity Leads to a Versatile Three-Dimensional Building Block. LID - e9476064 [pii] LID - 10.1002/anie.9476064 [doi] AB - Cyclooctatetraene (COT)-containing building blocks occupy a unique position in carbon materials, yet their synthesis generally relies on lengthy and low-yielding approaches. In this study, we report the serendipitous discovery and rational development of tetraxene, a synthetically accessible tetraindene-fused COT scaffold uncovered during attempts to extend truxene pi-conjugation. Although indanone condensations intrinsically favor thermodynamically reinforced trimer formation, stepwise interception and reactivation of a discrete dimer intermediate redirects the reaction toward selective tetramerization. This two-step strategy provides concise access to a family of tetraxene derivatives (2b-e) in synthetically useful yields (70%-80%). Single-crystal x-ray diffraction reveals a rigid nonaromatic COT core with pronounced bond localization while demonstrating that subtle peripheral modifications profoundly alter solid-state symmetry, leading to remarkably diverse supramolecular architectures. Beyond its distinctive structural behavior, tetraxene contains orthogonally addressable reactive domains that enable modular functionalization at both the aromatic periphery and the five-membered rings. These results establish tetraxene as a structurally defined and chemically versatile 3D COT-based building block and demonstrate that thermodynamically biased aromatic condensation pathways can be deliberately redirected toward higher-order non-benzenoid carbon architectures. CI - (c) 2026 Wiley-VCH GmbH. FAU - Vicent, Diego J AU - Vicent DJ AUID- ORCID: 0000-0003-3383-6691 AD - Departamento De Quimica Organica, Facultad de Ciencias Quimicas, Universidad Complutense, Madrid, Spain. FAU - Perles, Josefina AU - Perles J AUID- ORCID: 0000-0003-0256-0186 AD - Laboratorio DRX Monocristal, SIdI, Universidad Autonoma de Madrid, Madrid, Spain. FAU - Martin, Nazario AU - Martin N AUID- ORCID: 0000-0002-5355-1477 AD - Departamento De Quimica Organica, Facultad de Ciencias Quimicas, Universidad Complutense, Madrid, Spain. AD - IMDEA Nanoscience, Campus de Cantoblanco, Madrid, Spain. LA - eng GR - 951224/European Research Council, European Union's Horizon 2020 Research and Innovation Programme/ GR - PID2023-146373OB-I00/Spanish MICINN/ GR - CEX2020-001039-S/"Severo Ochoa" Programme for Centres of Excellence in R&D/ PT - Journal Article DEP - 20261027 PL - Germany TA - Angew Chem Int Ed Engl JT - Angewandte Chemie (International ed. in English) JID - 0370543 SB - IM OTO - NOTNLM OT - building block OT - cyclooctatetraene OT - non-benzenoid hydrocarbons OT - supramolecular organization OT - truxene EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 11:13 PHST- 2026/10/13 00:00 [revised] PHST- 2026/08/08 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:13 [entrez] AID - e9476064 [pii] AID - 10.1002/anie.9476064 [doi] PST - aheadofprint SO - Angew Chem Int Ed Engl. 2026 Oct 27:e9476064. doi: 10.1002/anie.9476064. PMID- 42944623 OWN - NLM STAT- Publisher LR - 20261027 IS - 1532-5415 (Electronic) IS - 0002-8614 (Linking) DP - 2026 Oct 27 TI - Low-Use vs. High-Use Physical Restraint in Older Adults With Sepsis Receiving Mechanical Ventilation: A Multicenter Randomized Clinical Trial. LID - 10.1111/jgs.70814 [doi] AB - BACKGROUND: Physical restraints are commonly used in patients receiving mechanical ventilation in the intensive care unit (ICU), yet evidence regarding their effectiveness in older patients with sepsis is limited. This study evaluated whether a low-use physical restraint strategy improves outcomes in older patients with sepsis requiring invasive mechanical ventilation. METHODS: This multicenter, open-label, randomized controlled trial was conducted in five ICUs in China from August 2023 to March 2026. Patients aged >/= 65 years with invasive mechanical ventilation were randomly assigned (1:1) to a low-use restraint strategy (restraints applied only for severe agitation) or a high-use restraint strategy (routine restraint use until recovery of consciousness and liberation from mechanical ventilation). The primary outcome was days alive without delirium or coma within 7 days after randomization. Secondary outcomes included delirium incidence, ventilator-free days, coma-free days, sedative exposure, 28-day mortality, and safety events. Analyses were performed using intention-to-treat, per-protocol, and inverse probability weighting approaches. RESULTS: Among 485 randomized patients (median age, 74 years; 60.4% male), 244 received low-use and 241 high-use restraint. Delirium- and coma-free days did not differ between groups (adjusted mean difference, 0.17 days; 95% CI, -0.20 to 0.54; p = 0.37). Low-use restraint was associated with more ventilator-free days (0.69 days; 95% CI, 0.48-0.91; p < 0.001) and coma-free days (0.46 days; 95% CI, 0.19-0.74; p = 0.001). No significant between-group differences were observed in delirium incidence, sedative exposure, 28-day mortality, or safety outcomes. CONCLUSIONS: Among older mechanically ventilated patients with sepsis, a low-use physical restraint strategy did not improve days alive without delirium or coma but was associated with more ventilator-free and coma-free days. These findings suggest that reducing restraint use may facilitate short-term recovery, although the safety of restraint minimization requires further evaluation. TRAIL REGISTRATION: ClinicalTrials.gov identifier: ChiCTR1900025850. CI - (c) 2026 The Author(s). Journal of the American Geriatrics Society published by Wiley Periodicals LLC on behalf of The American Geriatrics Society. FAU - Han, Yue AU - Han Y AD - Department of Critical Care Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China. FAU - Ma, Wenliang AU - Ma W AD - Department of Critical Care Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China. FAU - Wang, Jingyi AU - Wang J AD - Department of Critical Care Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China. FAU - Wang, Hao AU - Wang H AD - Department of Critical Care Medicine, Beijing Jishuitan Hospital, Beijing, China. FAU - Chang, Zhigang AU - Chang Z AUID- ORCID: 0000-0002-3125-0040 AD - Department of Critical Care Medicine, Beijing Hospital, Beijing, China. FAU - Wang, Yuefu AU - Wang Y AD - Surgical Intensive Care Unit, Beijing Shijitan Hospital, Capital Medical University, Beijing, China. FAU - Cui, Na AU - Cui N AUID- ORCID: 0000-0003-1128-9228 AD - Department of Critical Care Medicine, Beijing Chaoyang Hospital, Capital Medical University, Beijing, China. LA - eng GR - 320.6750.2024-22-6/Wu Jieping Medical Foundation/ GR - CYTS2025A03/Beijing Municipal Health Commission/ GR - JSWP2026-07/Beijing Health Promotion Association/ PT - Journal Article DEP - 20261027 PL - United States TA - J Am Geriatr Soc JT - Journal of the American Geriatrics Society JID - 7503062 SB - IM OTO - NOTNLM OT - delirium OT - mechanical ventilation OT - older patients OT - physical restraint OT - sepsis EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 11:13 PHST- 2026/10/08 00:00 [revised] PHST- 2026/06/14 00:00 [received] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:13 [entrez] AID - 10.1111/jgs.70814 [doi] PST - aheadofprint SO - J Am Geriatr Soc. 2026 Oct 27. doi: 10.1111/jgs.70814. PMID- 42944622 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1757-5672 (Electronic) IS - 1757-5664 (Linking) VI - 18 IP - 6 DP - 2026 Nov TI - Impact of Histopathology and Bladder Dysfunction on the Treatment Strategy Selection of Ketamine-Associated Cystitis. LID - e70102 [pii] LID - 10.1111/luts.70102 [doi] AB - OBJECTIVE: To investigate predictors for selecting an appropriate treatment strategy for ketamine-associated cystitis (KC) due to chronic ketamine abuse for entertainment. METHODS: This retrospective study included 62 patients with KC who underwent baseline videourodynamics and bladder cold-cup biopsy; 43 subsequently underwent AE and 19 continued conservative treatment. Histopathology-urodynamic associations were assessed using Spearman correlations. A parsimonious logistic model evaluated factors associated with subsequent AE. The exploratory five-item histopathological score was assessed in complete cases with bootstrap resampling. RESULTS: Patients subsequently undergoing AE had lower first sensation of filling, fullness sensation, compliance, Q(max), and voided volume, while cystoscopic maximal bladder capacity was not significantly different (p = 0.085). Urothelial denudation severity correlated with lower first sensation (rho = -0.36, p = 0.017) and fullness sensation (rho = -0.49, p < 0.001). In the multivariable model (N = 44), higher voided volume was associated with lower odds of AE (OR: 0.72 per 10 mL, 95% CI: 0.57-0.90, p = 0.004), whereas severe urothelial denudation was associated with higher odds (OR: 27.24, 95% CI: 1.73-428.36, p = 0.019). The complete-case score (N = 50) had AUC 0.941 (bootstrap 95% CI: 0.875-0.988); cutoff >/= 2 yielded 94.4% sensitivity and 78.6% specificity. CONCLUSION: Lower voided volume and severe urothelial denudation were associated with subsequent AE. The composite histopathological score is exploratory and requires external validation. CI - (c) 2026 John Wiley & Sons Australia, Ltd. FAU - Jhang, Jia-Fong AU - Jhang JF AUID- ORCID: 0000-0001-6674-2152 AD - Department of Urology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation and Tzu Chi University, Hualien, Taiwan. FAU - Yu, Wan-Ru AU - Yu WR AUID- ORCID: 0000-0001-9927-4092 AD - Department of Urology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation and Tzu Chi University, Hualien, Taiwan. FAU - Jiang, Yuan-Hong AU - Jiang YH AUID- ORCID: 0000-0002-7870-1574 AD - Department of Urology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation and Tzu Chi University, Hualien, Taiwan. FAU - Kuo, Hann-Chorng AU - Kuo HC AUID- ORCID: 0000-0001-7165-4771 AD - Department of Urology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation and Tzu Chi University, Hualien, Taiwan. LA - eng GR - TCMF-SP-115-01/Buddhist Tzu Chi Medical Foundation/ GR - TCMF-MP-114-02-01/Buddhist Tzu Chi Medical Foundation/ PT - Journal Article PL - Australia TA - Low Urin Tract Symptoms JT - Lower urinary tract symptoms JID - 101506777 RN - 690G0D6V8H (Ketamine) SB - IM MH - Humans MH - *Ketamine/adverse effects MH - *Cystitis/chemically induced/pathology/therapy/physiopathology MH - Female MH - Retrospective Studies MH - Male MH - *Urinary Bladder/pathology/physiopathology MH - Adult MH - Urodynamics MH - *Substance-Related Disorders/complications MH - Middle Aged MH - Biopsy OTO - NOTNLM OT - bladder inflammation OT - bladder pain syndrome OT - ketamine cystitis OT - treatment EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 CRDT- 2026/10/27 11:04 PHST- 2026/09/17 00:00 [revised] PHST- 2026/08/15 00:00 [received] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:04 [entrez] AID - e70102 [pii] AID - 10.1111/luts.70102 [doi] PST - ppublish SO - Low Urin Tract Symptoms. 2026 Nov;18(6):e70102. doi: 10.1111/luts.70102. PMID- 42944615 OWN - NLM STAT- Publisher LR - 20261027 IS - 1531-698X (Electronic) IS - 1040-8703 (Linking) DP - 2026 Oct 20 TI - Practical protocols and clinical experience from an academically based oral immunotherapy program. LID - 10.1097/MOP.0000000000001618 [doi] AB - PURPOSE OF REVIEW: Food allergy represents a significant public health burden with implications on quality of life and persistent risk of accidental allergic reactions. Therapeutic immunomodulation for food allergy has only recently entered clinical practice. Oral immunotherapy (OIT) has emerged as an effective approach for increasing allergen reaction thresholds; however, its implementation remains limited due to systemic factors. This review aims to describe the development, implementation, and outcomes of a standardized OIT program for immunoglobulin E (IgE)-mediated food allergy at an academic pediatric allergy institution. RECENT FINDINGS: An OIT program was developed with the objectives of improving accessibility, standardizing treatment approaches, minimizing adverse events, and allowing individualized treatment goals ranging from protection against accidental exposure to dietary liberalization. SUMMARY: OIT represents an effective, safe immunomodulatory treatment for IgE-mediated food allergy. The implementation of a standardized OIT program allowed expansion from an initial pilot program to a large clinical service involving thousands of patients. Both single-food and multifood OIT protocols were feasible and safe. Most adverse reactions were mild-moderate and gastrointestinal or cutaneous in nature. Severe reactions and eosinophilic esophagitis (EoE) were uncommon when patients were appropriately selected and monitored. Standardized protocols, careful patient selection, extensive counseling, and systematic monitoring are essential to optimize outcomes and minimize risks. CI - Copyright (c) 2026 Wolters Kluwer Health, LLC. All rights reserved. FAU - Buckey, Timothy M AU - Buckey TM AD - Division of Allergy and Immunology, Children's Hospital of Philadelphia. AD - Department of Pediatrics, Perelman School of Medicine. AD - Section of Allergy and Immunology, Division of Pulmonary, Allergy, and Critical Care Medicine. AD - Department of Medical Ethics and Health Policy, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA. FAU - Cianferoni, Antonella AU - Cianferoni A AD - Division of Allergy and Immunology, Children's Hospital of Philadelphia. AD - Department of Pediatrics, Perelman School of Medicine. LA - eng PT - Journal Article DEP - 20261020 PL - United States TA - Curr Opin Pediatr JT - Current opinion in pediatrics JID - 9000850 SB - IM OTO - NOTNLM OT - desensitization OT - eosinophilic esophagitis OT - food allergy OT - oral immunotherapy OT - sustained unresponsiveness EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 11:04 PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:04 [entrez] AID - 00008480-990000000-00401 [pii] AID - 10.1097/MOP.0000000000001618 [doi] PST - aheadofprint SO - Curr Opin Pediatr. 2026 Oct 20. doi: 10.1097/MOP.0000000000001618. PMID- 42944612 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2474-9842 (Electronic) IS - 2474-9842 (Linking) VI - 10 IP - 5 DP - 2026 Sep 4 TI - Robotic versus open pancreatoduodenectomy in patients with obesity: propensity score-matched multicentre study. LID - 10.1093/bjsopen/zrag107 [doi] LID - zrag107 AB - BACKGROUND: Obesity increases morbidity after major abdominal surgery. Although the use of robotic pancreatoduodenectomy (RPD) is increasing, evidence of its outcomes in patients with obesity remains limited. This study compared perioperative outcomes of RPD versus open pancreatoduodenectomy (OPD) in patients with obesity. METHODS: This was a retrospective multicentre cohort study of patients with a body mass index (BMI) >/= 30 kg/m2 undergoing RPD or OPD between 2012 and 2024. Propensity score matching (PSM) was used to minimize selection bias. The primary outcome was severe complications (Clavien-Dindo >/= IIIa). Secondary outcomes included blood loss, clinically relevant postoperative pancreatic fistula (CR-POPF), clinically relevant delayed gastric emptying (CR-DGE), wound infection, length of hospital stay (LOS), 90-day mortality, and R0 resection. Factors independently associated with severe complications and CR-POPF were investigated using multivariable logistic regression. Fistula risk was classified based on the Updated Alternate Fistula Risk Score (Pancreatic Fistula Risk Score, PFRS). RESULTS: In all, 591 patients from 31 centres were analysed. After PSM, 166 patients undergoing RPD were compared to 166 undergoing OPD. In the PSM cohort, RPD was associated with significantly lower rates of severe complications (15.7% versus 27.7%; P = 0.007), CR-POPF (8.4% versus 18%; P = 0.011), CR-DGE (9% versus 18.7%; P = 0.022), and wound infection (4.2% versus 21%; P < 0.001). Median blood loss was lower in the RPD than OPD group (100 versus 200 ml; P < 0.001), as was median LOS (11 versus 17 days; P < 0.001). No differences were observed in 90-day mortality or R0 resection rates. Charlson co-morbidity index (odds ratio (OR) 0.68, 95% confidence interval (CI) 0.59-0.83, P = 0.003), robotic approach (OR 0.39, 95%CI 0.18-0.60, P = 0.002), and institutional experience of >/= 20 robotic resections (OR 0.36, 95%CI 0.27-0.52, P = 0.007) were independently associated with lower CR-POPF risk, whereas a higher PFRS was associated with increased CR-POPF risk (OR 1.73, 95%CI 1.02-1.85, P = 0.032). Similarly, institutional experience of >/= 20 robotic resections was independently associated with a lower incidence of severe complications (OR 0.39, 95%CI 0.22-0.81, P = 0.003), whereas a higher PFRS independently predicted severe complications (OR 1.93, 95%CI 1.07-2.31, P = 0.006). CONCLUSIONS: RPD is a safe and effective alternative to OPD in patients with obesity when performed in experienced centres. These findings support the adoption of RPD in select high-BMI populations in experienced institutions. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of BJS Foundation Ltd. FAU - Valle, Valentina AU - Valle V AUID- ORCID: 0000-0002-8211-7370 AD - Division of General, Minimally Invasive, & Robotic Surgery, University of Illinois at Chicago, Illinois, USA. FAU - Marchese, Tiziana AU - Marchese T AD - General and HPB Surgery Department, Vito Fazzi Hospital, Lecce, Italy. FAU - Ielpo, Benedetto AU - Ielpo B AUID- ORCID: 0000-0003-3129-3208 AD - HPB Unit, Hospital Del Mar, Pompeu Fabra University, Barcelona, Spain. FAU - Comandatore, Annalisa AU - Comandatore A AD - General Surgery Unit, Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy. FAU - Spampinato, Marcello AU - Spampinato M AD - General and HPB Surgery Department, Vito Fazzi Hospital, Lecce, Italy. FAU - Borgioli, Leonardo AU - Borgioli L AD - Department of Electrical and Computer Engineering, College of Engineering, University of Illinois at Chicago, Chicago, Illinois, USA. FAU - Di Franco, Gregorio AU - Di Franco G AD - General Surgery Unit, Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy. FAU - Giulianotti, Pier Cristoforo AU - Giulianotti PC AD - Division of General, Minimally Invasive, & Robotic Surgery, University of Illinois at Chicago, Illinois, USA. FAU - Morelli, Luca AU - Morelli L AD - General Surgery Unit, Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy. CN - PANFRAIL Collaborative Group CN - on behalf of LA - eng PT - Comparative Study PT - Journal Article PT - Multicenter Study PL - England TA - BJS Open JT - BJS open JID - 101722685 SB - IM MH - Humans MH - *Pancreaticoduodenectomy/methods/adverse effects MH - Propensity Score MH - Female MH - Retrospective Studies MH - *Robotic Surgical Procedures/adverse effects/methods MH - *Postoperative Complications/epidemiology/etiology MH - Male MH - *Obesity/complications MH - Middle Aged MH - Length of Stay/statistics & numerical data MH - Aged MH - Pancreatic Fistula/epidemiology/etiology MH - Body Mass Index MH - Treatment Outcome PMC - PMC13711665 OTO - NOTNLM OT - minimally invasive surgery OT - postoperative pancreatic fistula OT - propensity score matching OT - robotic surgery OT - surgical outcomes FIR - De Pastena, M IR - De Pastena M FIR - Esposito, A IR - Esposito A FIR - Salvia, R IR - Salvia R FIR - Ietto, G IR - Ietto G FIR - Delpini, R IR - Delpini R FIR - Hackert, T IR - Hackert T FIR - Gehrisch, F IR - Gehrisch F FIR - Afshar-Bakshloo, K IR - Afshar-Bakshloo K FIR - G Uzunoglu, F IR - G Uzunoglu F FIR - Ross, S IR - Ross S FIR - Vanterpool, G IR - Vanterpool G FIR - Berrevoet, F IR - Berrevoet F FIR - Standaert, L IR - Standaert L FIR - Citterio, D IR - Citterio D FIR - Mazzaferro, V IR - Mazzaferro V FIR - Cereda, M IR - Cereda M FIR - Ishizawa, T IR - Ishizawa T FIR - Malpaga, A IR - Malpaga A FIR - Ciola, M IR - Ciola M FIR - Gasparin, F IR - Gasparin F FIR - Maroso, F IR - Maroso F FIR - Romito, R IR - Romito R FIR - Poletto, E IR - Poletto E FIR - Ruzzenente, A IR - Ruzzenente A FIR - Furbetta, N IR - Furbetta N FIR - Guadagni, S IR - Guadagni S FIR - Marchegiani, G IR - Marchegiani G FIR - Cobellis, F IR - Cobellis F FIR - Iacomino, A IR - Iacomino A FIR - Zanus, G IR - Zanus G FIR - Giannone, F IR - Giannone F FIR - Matarazzo, F IR - Matarazzo F FIR - Scheiterle, M IR - Scheiterle M FIR - Rhaiem, R IR - Rhaiem R FIR - Zimmermann, P IR - Zimmermann P FIR - Martinie, J IR - Martinie J FIR - Memba Ikuga, R IR - Memba Ikuga R FIR - Defez, M IR - Defez M FIR - Jovine, E IR - Jovine E FIR - Mastrangelo, L IR - Mastrangelo L FIR - Vicente, E IR - Vicente E FIR - Ferri, V IR - Ferri V FIR - Quijano, Y IR - Quijano Y FIR - Caruso, R IR - Caruso R FIR - Delis, S IR - Delis S FIR - Wray, C J IR - Wray CJ FIR - Maffeis, F IR - Maffeis F FIR - Perra, T IR - Perra T FIR - Porcu, A IR - Porcu A FIR - Anselmo, A IR - Anselmo A FIR - Conte, L E IR - Conte LE FIR - Giglio, M C IR - Giglio MC FIR - Troisi, R I IR - Troisi RI FIR - Dorma, M P F IR - Dorma MPF FIR - Rosso, E IR - Rosso E FIR - Fassari, A IR - Fassari A FIR - Barbato, G IR - Barbato G FIR - Belli, A IR - Belli A FIR - Memeo, R IR - Memeo R FIR - Filippo, R IR - Filippo R FIR - Garulli, G IR - Garulli G FIR - Giordano, M IR - Giordano M FIR - Machairas, N IR - Machairas N FIR - Masetti, M IR - Masetti M FIR - Offi, M F IR - Offi MF FIR - Sergeant, G IR - Sergeant G FIR - Depaifve, Y IR - Depaifve Y FIR - Goh, B K P IR - Goh BKP FIR - Koh, Y X IR - Koh YX FIR - Masuda, Y IR - Masuda Y EDAT- 2026/10/27 17:30 MHDA- 2026/10/27 17:31 PMCR- 2026/10/27 CRDT- 2026/10/27 11:04 PHST- 2025/12/11 00:00 [received] PHST- 2026/04/28 00:00 [revised] PHST- 2026/06/22 00:00 [accepted] PHST- 2026/10/27 17:31 [medline] PHST- 2026/10/27 17:30 [pubmed] PHST- 2026/10/27 11:04 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - 9003023 [pii] AID - zrag107 [pii] AID - 10.1093/bjsopen/zrag107 [doi] PST - ppublish SO - BJS Open. 2026 Sep 4;10(5):zrag107. doi: 10.1093/bjsopen/zrag107. PMID- 42944611 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2474-9842 (Electronic) IS - 2474-9842 (Linking) VI - 10 IP - 5 DP - 2026 Sep 4 TI - International expert consensus on perioperative management of carcinoid crisis. LID - 10.1093/bjsopen/zrag159 [doi] LID - zrag159 AB - BACKGROUND: Carcinoid crisis is a critical concern for patients with neuroendocrine tumours undergoing invasive procedures. Guidance is needed to standardize and optimize perioperative care for patients at risk of carcinoid crisis. Consensus-based expert recommendations were developed for the perioperative management of carcinoid crisis among patients with midgut and bronchopulmonary neuroendocrine tumours undergoing non-cardiac surgery. METHODS: A modified Delphi approach was used with four rounds of voting to reach expert consensus on statements about perioperative management of carcinoid crisis. A multidisciplinary international panel of 44 experts was formed, and 3 patient partners were engaged. Statements addressing preoperative, intraoperative, and postoperative considerations were created. Panellists rated statements on a Likert scale (from 1 to 7). Three additional rounds of iterative rating and feedback were completed anonymously. Consensus was defined with median score between 5 and 7, and 3 sites (69%) of osseous disease. A high proportion of patients had osseous metastases at sites with high risk for fracture, including vertebra (82%), pelvis (63%), and peri-trochanteric femur (34%). Fifty-seven patients (13%) in the entire cohort suffered a fracture. Fracture prevalence was significantly lower in small bowel neuroendocrine tumors (NETs, 7%) and Grades 1 and 2 disease (8% and 9%, respectively), with higher prevalence in pancreatic NETs, paragangliomas/pheochromocytomas, lung carcinoid, and Grade 3 disease (>/=20% for each; p-values <.05). Our study showed that the rate of osseous metastatic disease approximates 20% in well-differentiated NENs. Most bone metastases are asymptomatic and the risk of fracture is variable based on the primary site of disease and tumor grade. Our novel risk score analysis for fracture was designed to assist clinician decision making as to management of bone metastasis in this patient population. CI - (c) 2026 The Author(s). Journal of Neuroendocrinology published by John Wiley & Sons Ltd on behalf of British Society for Neuroendocrinology. FAU - Coston, Tucker W AU - Coston TW AUID- ORCID: 0000-0002-5843-2506 AD - Division of Hematology and Oncology, Duke Cancer Center, Durham, North Carolina, USA. FAU - Mahadevia, Himil J AU - Mahadevia HJ AUID- ORCID: 0000-0002-2083-5510 AD - Division of Hematology and Oncology, Mayo Clinic in Jacksonville, Jacksonville, Florida, USA. FAU - Plante, Marie M AU - Plante MM AD - Division of Hematology and Oncology, Wake Forest Baptist Medical Center, Winston-Salem, North Carolina, USA. FAU - Hochwald, Alex AU - Hochwald A AUID- ORCID: 0009-0009-3651-746X AD - Division of Clinical Trials and Biostatistics, Mayo Clinic in Jacksonville, Jacksonville, Florida, USA. FAU - Accurso, Joseph M AU - Accurso JM AUID- ORCID: 0000-0001-5065-0325 AD - Division of Nuclear Medicine, Mayo Clinic in Jacksonville, Jacksonville, Florida, USA. FAU - Sharma, Akash AU - Sharma A AUID- ORCID: 0000-0003-4017-9132 AD - Division of Nuclear Medicine, Mayo Clinic in Jacksonville, Jacksonville, Florida, USA. FAU - Johnson, Geoffrey B AU - Johnson GB AUID- ORCID: 0000-0002-9781-4072 AD - Division of Nuclear Medicine, Mayo Clinic in Rochester, Rochester, Minnesota, USA. FAU - Ashman, Jonathan B AU - Ashman JB AD - Department of Radiation Oncology, Mayo Clinic in Phoenix, Phoenix, Arizona, USA. FAU - Kendi, A Tuba AU - Kendi AT AD - Division of Nuclear Medicine, Mayo Clinic in Rochester, Rochester, Minnesota, USA. FAU - Sonbol, Mohamad Bassam AU - Sonbol MB AUID- ORCID: 0000-0002-6931-242X AD - Division of Hematology and Oncology, Mayo Clinic in Phoenix, Phoenix, Arizona, USA. FAU - McGarrah, Patrick W AU - McGarrah PW AUID- ORCID: 0000-0002-0664-9447 AD - Division of Hematology and Oncology, Mayo Clinic in Rochester, Rochester, Minnesota, USA. FAU - Hobday, Timothy J AU - Hobday TJ AD - Division of Hematology and Oncology, Mayo Clinic in Rochester, Rochester, Minnesota, USA. FAU - Halfdanarson, Thorvardur R AU - Halfdanarson TR AUID- ORCID: 0000-0001-8460-1257 AD - Division of Hematology and Oncology, Mayo Clinic in Rochester, Rochester, Minnesota, USA. FAU - Starr, Jason S AU - Starr JS AUID- ORCID: 0000-0002-8674-8102 AD - Division of Hematology and Oncology, Duke Cancer Center, Durham, North Carolina, USA. LA - eng GR - Endocyte/ GR - Novartis/ PT - Journal Article PL - United States TA - J Neuroendocrinol JT - Journal of neuroendocrinology JID - 8913461 RN - 0 (Organometallic Compounds) RN - 9L17Y0H71P (gallium Ga 68 dotatate) SB - IM MH - Humans MH - *Neuroendocrine Tumors/pathology/diagnostic imaging MH - Female MH - *Bone Neoplasms/secondary/diagnostic imaging/epidemiology MH - Organometallic Compounds MH - Male MH - Middle Aged MH - Retrospective Studies MH - Aged MH - *Positron-Emission Tomography/methods MH - Adult MH - Fractures, Bone/epidemiology/diagnostic imaging/etiology MH - Aged, 80 and over PMC - PMC13712210 OTO - NOTNLM OT - 68Ga-DOTATATE OT - PET/CT OT - bone metastasis OT - neuroendocrine neoplasm OT - neuroendocrine tumor COIS- ATK receives research funding from Endocyte (now owned by Novartis); TJH receives research funding from Novartis. TRH is a consultant for Advanced Accelerator Applications (Inst), Crinetics Pharmaceuticals (Inst), Ipsen (Inst), ITM Isotope Technologies Munich (Inst), Tersera, TERUMO, and Viewpoint Molecular Targeting (Inst). TRH receives research funding from Advanced Accelerator Applications (Inst), Agios (Inst), Basilea (Inst), Ipsen (Inst), Novartis (Inst), Thermo Fisher Scientific (Inst), Turnstone Bio (Inst). JSS is a consultant for Advanced Accelerator Applications, Boehringer-Ingelheim, Ipsen, Natera, Pfizer, Taiho Oncology, Tersera, Helsinn Therapeutics, and Cancer Expert Now. JSS received travel-related funding from Camurus. JSS receives research funding from Leap Therapeutics (Inst), Macrogenics (Inst), Merus (Inst), Molecular Templates (Inst), Rafael Pharmaceuticals (Inst), Amgen (Inst.), Camurus (Inst.), Perspective Therapeutics (Inst.), and Boehringer-Ingelheim (Inst.). Other authors declare no conflicts of interests. EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 17:30 PMCR- 2026/10/27 CRDT- 2026/10/27 10:52 PHST- 2026/10/07 00:00 [revised] PHST- 2026/07/10 00:00 [received] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:52 [entrez] PHST- 2026/10/27 00:00 [pmc-release] AID - e70301 [pii] AID - JNE70301 [pii] AID - 10.1111/jne.70301 [doi] PST - ppublish SO - J Neuroendocrinol. 2026 Nov;38(11):e70301. doi: 10.1111/jne.70301. PMID- 42944556 OWN - NLM STAT- Publisher LR - 20261027 IS - 1872-6623 (Electronic) IS - 0304-3959 (Linking) DP - 2026 Oct 26 TI - Mineralocorticoid receptor signaling reduces somatostatin expression in developing sensory neurons and drives neonatal pain after early-life stress. LID - 10.1097/j.pain.0000000000004139 [doi] AB - Early-life stress (ELS) causes pain hypersensitivity in neonatal mice and alters the transcriptional profile of the immature dorsal root ganglia (DRG), highlighted by a marked reduction in the expression of a neuropeptide, somatostatin (Sst), which is known to constitutively suppress peripheral nociceptive transmission during adulthood. However, the mechanisms that drive plasticity in gene expression within the developing DRG and neonatal pain after ELS remain unclear. Here, we investigated the degree to which ELS-induced neonatal pain was mediated by corticosterone signaling onto sensory neurons and potential changes in peripheral Sst transmission. The conditional knockout of the mineralocorticoid receptor (MR), but not the glucocorticoid receptor, from immature DRG neurons prevented ELS-evoked neonatal pain. In contrast, the systemic administration of the selective MR antagonist eplerenone failed to acutely influence pain after ELS, thus suggesting that MR activation enhances pain during the neonatal period via canonical effects on gene transcription. Meanwhile, hind paw injection of the Sst receptor antagonist cyclo-somatostatin increased spontaneous nocifensive behaviors in control pups, but not in pups previously subjected to ELS, raising the possibility that a disruption in tonic antinociception mediated by peripheral Sst contributes to neonatal pain after ELS. Furthermore, administration of the peripherally restricted Sst analog octreotide reversed noxious cold and pressure hypersensitivity in neonatal mice after ELS. Finally, the selective deletion of MR from sensory neurons prevented the ELS-induced decrease in Sst expression. Collectively, these results provide novel mechanistic insight into the effects of chronic early-life adversity on nociceptive processing in the developing nervous system. CI - Copyright (c) 2026 International Association for the Study of Pain. FAU - Eid, Fady AU - Eid F AD - Neuroscience Graduate Program, University of Cincinnati College of Medicine, Cincinnati, OH, United States. AD - Pain Research Center, Department of Anesthesiology, University of Cincinnati College of Medicine, Cincinnati, OH, United States. FAU - Serafin, Elizabeth AU - Serafin E AD - Pain Research Center, Department of Anesthesiology, University of Cincinnati College of Medicine, Cincinnati, OH, United States. FAU - Muniz, Mezani AU - Muniz M AD - Research Innovation in Neuroscience Education for Underserved Populations Undergraduate Fellowship, University of Cincinnati College of Medicine, Cincinnati, OH, United States. FAU - Woodke, Samantha AU - Woodke S AD - Pain Research Center, Department of Anesthesiology, University of Cincinnati College of Medicine, Cincinnati, OH, United States. AD - Medical Scientist Training Program, University of Cincinnati College of Medicine, Cincinnati, OH, United States. FAU - Booher, Jacob AU - Booher J AD - Pain Research Center, Department of Anesthesiology, University of Cincinnati College of Medicine, Cincinnati, OH, United States. FAU - Zhang, Jun-Ming AU - Zhang JM AD - Neuroscience Graduate Program, University of Cincinnati College of Medicine, Cincinnati, OH, United States. AD - Pain Research Center, Department of Anesthesiology, University of Cincinnati College of Medicine, Cincinnati, OH, United States. FAU - Baccei, Mark L AU - Baccei ML AUID- ORCID: 0000-0003-3337-7480 AD - Neuroscience Graduate Program, University of Cincinnati College of Medicine, Cincinnati, OH, United States. AD - Pain Research Center, Department of Anesthesiology, University of Cincinnati College of Medicine, Cincinnati, OH, United States. AD - Research Innovation in Neuroscience Education for Underserved Populations Undergraduate Fellowship, University of Cincinnati College of Medicine, Cincinnati, OH, United States. AD - Medical Scientist Training Program, University of Cincinnati College of Medicine, Cincinnati, OH, United States. LA - eng GR - NS080889/NH/NIH HHS/United States PT - Journal Article DEP - 20261026 PL - United States TA - Pain JT - Pain JID - 7508686 SB - IM OTO - NOTNLM OT - Chronic stress OT - Corticosterone OT - Dorsal root ganglia (DRG) OT - Early-life adversity OT - Mouse OT - Neuropeptide OT - Pediatric pain EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 12:52 CRDT- 2026/10/27 10:52 PHST- 2026/06/30 00:00 [received] PHST- 2026/09/09 00:00 [accepted] PHST- 2026/10/27 12:52 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:52 [entrez] AID - 00006396-990000000-01290 [pii] AID - 10.1097/j.pain.0000000000004139 [doi] PST - aheadofprint SO - Pain. 2026 Oct 26. doi: 10.1097/j.pain.0000000000004139. PMID- 42944555 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261027 IS - 1938-3673 (Electronic) IS - 0741-5400 (Linking) VI - 118 IP - 10 DP - 2026 Oct 5 TI - SOX2/TSTA3 suppresses CD8+ T-cell-mediated immune response in breast cancer by regulating programmed death ligand-1 fucosylation. LID - qiag117 [pii] LID - 10.1093/jleuko/qiag117 [doi] AB - Immune escape in breast cancer is a primary contributor to immunotherapy resistance and poor patient prognosis. Fucosylation is essential for tumor progression, but the regulatory mechanism of its key enzyme, guanosine diphosphate-L-fucose synthase (TSTA3), within the breast cancer immune microenvironment remains unexplored. This study combined bioinformatics and experimental approaches to investigate TSTA3 in breast cancer. TSTA3 expression was validated by quantitative real-time reverse transcription polymerase chain reaction and western blotting. Functional assays in a tumor-T-cell coculture system showed that TSTA3 knockdown enhanced CD8+ T-cell cytotoxicity and suppressed tumor malignancy. Mechanistically, TSTA3 fucosylated programmed death ligand-1, and this modification was confirmed by Ulex europaeus agglutinin-1 chromatography and co-immunoprecipitation. SOX2 was identified as an upstream transcription factor of TSTA3 via chromatin immunoprecipitation and dual-luciferase assays. The SOX2/TSTA3/programmed death ligand-1 axis was functionally validated in rescue experiments and in vivo tumor models. TSTA3 was highly expressed in breast cancer tumors. Its knockdown enhanced CD8+ T-cell cytotoxicity and suppressed tumor proliferation, migration, and invasion in vitro. Mechanistically, TSTA3 upregulated programmed death ligand-1 expression in a fucose-dependent manner and, partly through programmed death ligand-1, suppressed CD8+ T-cell-mediated antitumor immune responses. SOX2 directly activated TSTA3 transcription, leading to programmed death ligand-1 stabilization via fucosylation. In vivo, targeting the SOX2/TSTA3/programmed death ligand-1 axis inhibited tumor growth and boosted CD8+ T-cell activity. Collectively, this study uncovered a novel mechanism whereby SOX2 transcriptionally activated TSTA3, which fucosylated programmed death ligand-1 and, partly through programmed death ligand-1, suppressed CD8+ T-cell-mediated antitumor immune responses, providing a theoretical basis for targeting the SOX2/TSTA3/programmed death ligand-1 axis to enhance immunotherapy in breast cancer. CI - (c) The Author(s) 2026. Published by Oxford University Press on behalf of Society for Leukocyte Biology. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for further information please contact journals.permissions@oup.com. FAU - Hong, Jia AU - Hong J AUID- ORCID: 0009-0001-6584-6141 AD - Department of Science and Education, Chun'an First People's Hospital, Hangzhou, Zhejiang Province 311700, China. FAU - Shao, Su AU - Shao S AD - Department of General Surgery, Chun'an First People's Hospital, Hangzhou, Zhejiang Province 311700, China. LA - eng GR - 2025KY1246/Zhejiang Province's Medical and Health Science and Technology Program/ PT - Journal Article PL - England TA - J Leukoc Biol JT - Journal of leukocyte biology JID - 8405628 RN - 0 (B7-H1 Antigen) RN - 0 (SOXB1 Transcription Factors) RN - 0 (CD274 protein, human) RN - 28RYY2IV3F (Fucose) SB - IM MH - Female MH - Humans MH - *CD8-Positive T-Lymphocytes/immunology/metabolism MH - *B7-H1 Antigen/metabolism MH - Animals MH - *Breast Neoplasms/immunology/pathology/metabolism/genetics MH - *SOXB1 Transcription Factors/metabolism/genetics MH - Mice MH - Cell Line, Tumor MH - Gene Expression Regulation, Neoplastic MH - *Fucose/metabolism MH - Cell Proliferation MH - Tumor Microenvironment/immunology OTO - NOTNLM OT - CD8+ T cells OT - PD-L1 OT - TSTA3 OT - breast cancer OT - fucosylation COIS- Conflicts of interests: None declared. EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 17:30 CRDT- 2026/10/27 10:52 PHST- 2026/02/27 00:00 [received] PHST- 2026/06/08 00:00 [revised] PHST- 2026/08/07 00:00 [accepted] PHST- 2026/10/27 17:30 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:52 [entrez] AID - 9002984 [pii] AID - 10.1093/jleuko/qiag117 [doi] PST - ppublish SO - J Leukoc Biol. 2026 Oct 5;118(10):qiag117. doi: 10.1093/jleuko/qiag117. PMID- 42944551 OWN - NLM STAT- Publisher LR - 20261027 IS - 1872-6623 (Electronic) IS - 0304-3959 (Linking) DP - 2026 Oct 27 TI - Astrocyte Orai1 signaling promotes spinal excitatory synaptic potentiation and neuropathic pain after nerve injury in male mice. LID - 10.1097/j.pain.0000000000004136 [doi] AB - Neuropathic pain after peripheral nerve injury is a multifactorial condition mediated partly by inflammation in the spinal cord that results in potentiation of excitatory synaptic transmission in nociceptive circuits. Glial cells including astrocytes are key mediators of the underlying inflammation, but the signaling checkpoints that regulate astrocyte-driven spinal cord inflammation and sensitization of nociceptive circuits are not well understood. Here, we identify Orai1-mediated store-operated Ca2+ entry (SOCE) as an important regulator of spinal astrocyte cytokine production, metabolic reprogramming, and pain hypersensitivity in mice. Conditional deletion of Orai1 in astrocytes attenuated mechanical allodynia after nerve injury in male but not female mice, despite comparable SOCE suppression in both sexes. Orai1 deficiency also attenuated production of proinflammatory cytokines after SOCE activation, including TNFalpha, IL-6, and IL-33, both in vivo and in vitro. Electrophysiological recordings revealed that astrocyte Orai1 deletion prevented injury-induced enhancement of glutamatergic synaptic transmission in dorsal horn neurons, linking astrocytic Ca2+ signaling to central sensitization. Metabolomic profiling showed that Orai1 loss disrupted glycolysis and TCA cycle intermediates while promoting accumulation of immunomodulatory metabolites such as itaconate, consistent with a less inflammatory astrocyte metabolic state. These findings identify Orai1 channels as integrators of astrocyte Ca2+ signaling, immunometabolism, and excitatory synaptic potentiation after nerve injury, and identify a sexually dimorphic astrocyte mechanism governing neuropathic pain pathogenesis. Targeting astrocyte Orai1 channels may provide a selective therapeutic strategy for male-predominant chronic pain. CI - Copyright (c) 2026 International Association for the Study of Pain. FAU - Tsujikawa, Shogo AU - Tsujikawa S AD - Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, IL, United States. Dr. Tsujikawa is now with Department of Anesthesiology, Osaka Metropolitan University Graduate School of Medicine, Osaka, Japan. FAU - Shetti, Aashutosh AU - Shetti A AD - Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, IL, United States. Dr. Tsujikawa is now with Department of Anesthesiology, Osaka Metropolitan University Graduate School of Medicine, Osaka, Japan. FAU - Grant, Rogan A AU - Grant RA AD - Division of Pulmonary and Critical Care, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL, United States. FAU - Novakovic, Michaela M AU - Novakovic MM AD - Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, IL, United States. Dr. Tsujikawa is now with Department of Anesthesiology, Osaka Metropolitan University Graduate School of Medicine, Osaka, Japan. FAU - Prakriya, Murali AU - Prakriya M AUID- ORCID: 0000-0003-0781-4480 AD - Department of Pharmacology, Northwestern University Feinberg School of Medicine, Chicago, IL, United States. Dr. Tsujikawa is now with Department of Anesthesiology, Osaka Metropolitan University Graduate School of Medicine, Osaka, Japan. LA - eng GR - R35NS132349/NH/NIH HHS/United States PT - Journal Article DEP - 20261027 PL - United States TA - Pain JT - Pain JID - 7508686 SB - IM OTO - NOTNLM OT - Astrocytes OT - Calcium signaling OT - Inflammation OT - Neuropathic pain OT - Orai1 EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 12:52 CRDT- 2026/10/27 10:52 PHST- 2026/03/11 00:00 [received] PHST- 2026/08/26 00:00 [accepted] PHST- 2026/10/27 12:52 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:52 [entrez] AID - 00006396-990000000-01291 [pii] AID - 10.1097/j.pain.0000000000004136 [doi] PST - aheadofprint SO - Pain. 2026 Oct 27. doi: 10.1097/j.pain.0000000000004136. PMID- 42944537 OWN - NLM STAT- Publisher LR - 20261027 IS - 1477-9145 (Electronic) IS - 0022-0949 (Linking) DP - 2026 Oct 27 TI - Constraints on oviposition learning: innate preference, cue integration and experience in a generalist fruit fly. LID - jeb.252395 [pii] LID - 10.1242/jeb.252395 [doi] AB - Learning enables animals to adjust behavior in changing environments, yet it may be constrained by innate preferences and prior experience. We examined how these factors interact in oviposition site selection-a key fitness-related trait-in the highly polyphagous Mediterranean fruit fly, Ceratitis capitata. We addressed three questions: (i) whether learning depends on the strength of innate preferences; (ii) how visual and olfactory modalities contribute to learning; and (iii) whether egg laying is required to induce persistent preference changes. Using 3D-printed artificial fruits differing in color and olfactory cues, we showed that oviposition behavior was highly plastic but strongly constrained. Learning depended on innate preference strength: it was limited when innate preferences were strong, yet facilitated when they were weak. Both visual and olfactory cues supported learning, but only when the identity of both stimuli was consistent between conditioning and test phases. Importantly, the absence of egg-laying opportunity induced avoidance of associated sensory stimuli rather than attraction to them when oviposition opportunity is present, indicating that the outcome of an oviposition attempt strongly influences subsequent oviposition preferences. Our findings show that oviposition learning results from interactions among innate preferences, sensory integration, and associative reinforcement. These constraints have implications for understanding how generalist insects can use various host species throughout their life and how environments shape those decisions. In particular, it underlines that integrating experience-dependent changes in behavior is needed to improve behavioral pest management strategies. CI - (c) 2026. Published by The Company of Biologists. FAU - Paillard, Emma AU - Paillard E AD - CBGP, INRAE, IRD, CIRAD, Institut Agro, University of Montpellier, 755 avenue du Campus Agropolis, CS 34988, Montferrier sur Lez cedex 30016, France. FAU - Foucaud, Julien AU - Foucaud J AUID- ORCID: 0000-0003-2272-3149 AD - CBGP, INRAE, IRD, CIRAD, Institut Agro, University of Montpellier, 755 avenue du Campus Agropolis, CS 34988, Montferrier sur Lez cedex 30016, France. FAU - Robejean, Myriam AU - Robejean M AUID- ORCID: 0009-0005-6285-0582 AD - CBGP, INRAE, IRD, CIRAD, Institut Agro, University of Montpellier, 755 avenue du Campus Agropolis, CS 34988, Montferrier sur Lez cedex 30016, France. FAU - Serrate, Bruno AU - Serrate B AD - CBGP, INRAE, IRD, CIRAD, Institut Agro, University of Montpellier, 755 avenue du Campus Agropolis, CS 34988, Montferrier sur Lez cedex 30016, France. FAU - Fraimout, Antoine AU - Fraimout A AD - CBGP, INRAE, IRD, CIRAD, Institut Agro, University of Montpellier, 755 avenue du Campus Agropolis, CS 34988, Montferrier sur Lez cedex 30016, France. FAU - Facon, Benoit AU - Facon B AUID- ORCID: 0000-0001-8970-6840 AD - CBGP, INRAE, IRD, CIRAD, Institut Agro, University of Montpellier, 755 avenue du Campus Agropolis, CS 34988, Montferrier sur Lez cedex 30016, France. LA - eng GR - PARSADA/ PT - Journal Article DEP - 20261027 PL - England TA - J Exp Biol JT - The Journal of experimental biology JID - 0243705 SB - IM OTO - NOTNLM OT - Associative learning OT - Behavior-based pest management OT - Oviposition preference OT - Phytophagous insects EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 12:52 CRDT- 2026/10/27 10:48 PHST- 2026/02/19 00:00 [received] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 12:52 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:48 [entrez] AID - 373240 [pii] AID - 10.1242/jeb.252395 [doi] PST - aheadofprint SO - J Exp Biol. 2026 Oct 27:jeb.252395. doi: 10.1242/jeb.252395. PMID- 42944528 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 21 TI - A Multimodal IMU-RTLS Manufacturing Motion Dataset for Worker Activity Recognition. LID - 10.3390/s26206665 [doi] LID - 6665 AB - Worker Activity Recognition can improve operational visibility in human-centric manufacturing systems. However, existing approaches remain limited by scarce industrial datasets, isolated task recordings, and sensor-intensive configurations. This study presents two multimodal datasets and a cyber-physical architecture for complementary levels of worker motion. The Gross Worker Activity Recognition dataset targets operator proximity to workstation and the identification of logistic activities in reconfigurable manufacturing systems. It contains 814,065 positioning samples from eight production setups, each repeated twice, involving more than 40 workers. This dataset is benchmarked with different learning structure algorithms, and the Long Short-Term Memory is selected as a baseline model, achieving an F1-score of 86%. The Fine Worker Activity Recognition dataset targets the segmentation of five pump-assembly operations using two inertial measurement units and a radio-frequency-identification smart glove. It comprises approximately 7 h of recordings and more than one million samples from 50 complete assemblies performed by three workers. Transformer, ResNet, and SqueezeNet models are benchmarked, with SqueezeNet selected as the baseline after achieving the highest F1-score of 85.2%. Moreover, the results identify transition-rich activities as the principal recognition bottleneck and show how complementary motion levels support different manufacturing indicators. FAU - Sbaragli, Andrea AU - Sbaragli A AUID- ORCID: 0000-0001-5014-2523 AD - School of Engineering, Newcastle University, Newcastle Upon Tyne NE1 7RU, UK. FAU - Nardello, Matteo AU - Nardello M AUID- ORCID: 0000-0003-3126-1177 AD - Department of Industrial Engineering, University of Trento, Via Sommarive 9, 38123 Trento, Italy. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM MH - Humans MH - Algorithms MH - Long Short Term Memory MH - *Motion MH - Pattern Recognition, Automated MH - *Manufacturing Industry PMC - PMC13709466 OTO - NOTNLM OT - IMU OT - RTLS OT - human-centric manufacturing OT - machine learning OT - worker activity recognition COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 22:06 PMCR- 2026/10/21 CRDT- 2026/10/27 10:48 PHST- 2026/09/21 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:48 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - s26206665 [pii] AID - sensors-26-06665 [pii] AID - 10.3390/s26206665 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 21;26(20):6665. doi: 10.3390/s26206665. PMID- 42944524 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 21 TI - Optimal Sensor Placement for Bridge Structural Health Monitoring: A Systematic Review with Bibliometric Analysis and Methodological Quality Assessment. LID - 10.3390/s26206661 [doi] LID - 6661 AB - Optimal Sensor Placement (OSP) is a key step in the design of Structural Health Monitoring (SHM) systems for bridges and requires combining civil engineering constraints with computational optimization. This systematic review, conducted following PRISMA 2020 guidelines, analyzes 49 studies through bibliometric analysis, a synthesis of optimization strategies, sensor modalities and research motivations, and a tailored Quality Assessment rubric. Accordingly, its quantitative results describe publication patterns, coded categories and methodological quality scores rather than bridge-specific dynamic parameters. Accelerometers are the most frequently used sensor modality, and improving data quality is the most frequently reported motivation (45 of 49 studies), whereas cost reduction, communication efficiency and energy efficiency appear in 11 studies each. Only 15 studies included experimental or field validation on physical bridges, and robustness against uncertainty was the weakest quality dimension, revealing a persistent simulation-to-reality gap. To organize these findings, the Sensor Placement Optimization Maturity Levels (SPO-ML) typology and a Unified Operational Decision Framework are proposed as literature-informed, preliminary guidance relating algorithm selection to structural complexity and network constraints. Future OSP research should prioritize field validation, robustness under uncertainty, and reproducible reporting of bridge and instrumentation characteristics. FAU - Rodriguez Wlack, Gaston Luis AU - Rodriguez Wlack GL AUID- ORCID: 0009-0001-8394-1337 AD - Pontificia Universidad Catolica de Valparaiso, Avenida Brasil 2147, Valparaiso 2362807, Chile. FAU - Fuentes, Nicolas AU - Fuentes N AUID- ORCID: 0009-0006-6606-1692 AD - Escuela de Ingenieria Informatica, Pontificia Universidad Catolica de Valparaiso, Avenida Brasil 2241, Valparaiso 2362807, Chile. FAU - Pena Fritz, Alvaro AU - Pena Fritz A AUID- ORCID: 0000-0003-2018-1972 AD - Pontificia Universidad Catolica de Valparaiso, Avenida Brasil 2147, Valparaiso 2362807, Chile. FAU - Crawford, Broderick AU - Crawford B AUID- ORCID: 0000-0001-5500-0188 AD - Escuela de Ingenieria Informatica, Pontificia Universidad Catolica de Valparaiso, Avenida Brasil 2241, Valparaiso 2362807, Chile. FAU - Soto, Ricardo AU - Soto R AUID- ORCID: 0000-0002-5755-6929 AD - Escuela de Ingenieria Informatica, Pontificia Universidad Catolica de Valparaiso, Avenida Brasil 2241, Valparaiso 2362807, Chile. LA - eng PT - Journal Article PT - Review PT - Systematic Review DEP - 20261021 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM MH - Accelerometry/instrumentation MH - *Algorithms MH - *Bibliometrics MH - *Structure Collapse/prevention & control MH - Thermometers MH - Optical Fibers PMC - PMC13709381 OTO - NOTNLM OT - bridge monitoring OT - damage detection OT - metaheuristic optimization OT - optimal sensor placement OT - sensor placement OT - structural health monitoring OT - vibration COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 22:06 PMCR- 2026/10/21 CRDT- 2026/10/27 10:48 PHST- 2026/08/14 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:48 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - s26206661 [pii] AID - sensors-26-06661 [pii] AID - 10.3390/s26206661 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 21;26(20):6661. doi: 10.3390/s26206661. PMID- 42944516 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 21 TI - Quantitative Evaluation of OpenCV Visual Tracking Algorithms for Drawn Markers Under Dynamic Facial Deformation. LID - 10.3390/s26206653 [doi] LID - 6653 AB - This study evaluates the eleven visual tracking algorithms available in OpenCV for tracking hand-drawn markers on deforming human faces. Using 41,641 multi-camera frames of seven subjects performing exaggerated phonetic and emotional movements, we assess each tracker's deviation from an automated Blob Detection reference (four-marker sequences), its long-term marker identity retention (forty-marker sequences), and its throughput. Of the tested classical trackers, the Channel and Spatial Reliability Tracker (CSRT) performed best, retaining 96.2% of markers with a mean deviation of 1.94+/-1.29 pixels (approximately 0.46+/-0.31 mm) at 56.9 frames per second (FPS). The Kernelized Correlation Filter (KCF) matched this deviation at 445.4 FPS but failed under transient self-occlusion, retaining only 64.1%. Among the neural trackers, the Vision Transformer-based tracker (ViT) recorded the smallest deviation (1.06+/-1.03 pixels, approximately 0.25+/-0.25 mm) at 164.1 FPS, and the Distractor-Aware Siamese Region Proposal Network (DaSiamRPN) had the highest retention (92.4%, 1.35+/-1.26 pixels) at 11.6 FPS. Differences among the leading trackers were not significant at the subject level. The Minimum Output Sum of Squared Error (MOSSE) tracker reached about 12,500 FPS, but retained only 49.6%. Per-frame deviation and long-term retention are decoupled, meaning that both must inform tracker selection; scenario-based recommendations are provided. FAU - Tkaczyk, Konrad AU - Tkaczyk K AD - Institute of Micromechanics and Photonics, Faculty of Mechatronics, Warsaw University of Technology, ul. Sw. Andrzeja Boboli 8, 02-525 Warsaw, Poland. FAU - Sitnik, Robert AU - Sitnik R AUID- ORCID: 0000-0002-8156-5462 AD - Institute of Micromechanics and Photonics, Faculty of Mechatronics, Warsaw University of Technology, ul. Sw. Andrzeja Boboli 8, 02-525 Warsaw, Poland. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM MH - Humans MH - *Algorithms MH - *Face/physiology/anatomy & histology MH - *Image Processing, Computer-Assisted/methods MH - Motion Capture MH - Detection Algorithms MH - Pattern Recognition, Automated/methods PMC - PMC13709949 OTO - NOTNLM OT - CSRT OT - DaSiamRPN OT - OpenCV OT - ViT OT - computer vision OT - non-rigid surface deformation OT - passive markers OT - tracking evaluation OT - tracking robustness OT - visual tracking COIS- The authors declare the following conflict of interest: Robert Sitnik is a shareholder and the Chief Technology Officer (CTO) of Mnemosis, the company that developed and provided the multi-camera 4D coordinate acquisition system used in this study [73]. The company Mnemosis had no role in the design of the study, in the collection, analysis, or interpretation of data, in the writing of the manuscript, or in the decision to publish the results. Konrad Tkaczyk declares no conflict of interest. EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 22:06 PMCR- 2026/10/21 CRDT- 2026/10/27 10:48 PHST- 2026/09/24 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:48 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - s26206653 [pii] AID - sensors-26-06653 [pii] AID - 10.3390/s26206653 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 21;26(20):6653. doi: 10.3390/s26206653. PMID- 42944515 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 21 TI - Sampling Frequency Requirements for Estimating Physical Activity Metrics from Wearable Accelerometer Data. LID - 10.3390/s26206652 [doi] LID - 6652 AB - Accelerometers are widely used to quantify physical activity in population health research, yet the sampling frequency required to preserve commonly reported measures remains uncertain. We evaluated sampling frequency using 315.4 h of labelled tri-axial accelerometer data from 321 participants across 11 publicly available datasets. Signals were harmonized to a 50 Hz reference and downsampled to 40, 30, 20, 10, and 5 Hz using anti-aliased resampling. We compared Monitor-Independent Movement Summary (MIMS), Euclidean Norm Minus One (ENMO), Activity Index (AI), dominant spectral frequency, and retained spectral power across activities and sensor locations. For MIMS, 20 Hz remained within the prespecified 5% error margin for many walking, stair-climbing, cycling, jumping, household activities, and motorized-transportation conditions, whereas running exceeded this margin at 20 Hz at lower-limb and trunk locations. At 10 Hz, errors increased for most ambulatory activities, and only about 60-72% of reference spectral power was retained for walking and running, despite preservation of their dominant movement frequencies. MIMS error was not associated with age, sex/gender, or BMI, although small sex/gender associations were observed for ENMO and AI. Sampling-frequency requirements, therefore, depend on the metric, activity, and sensor location, supporting fit-for-purpose selection rather than use of a single universal sampling rate. FAU - Straczkiewicz, Marcin AU - Straczkiewicz M AUID- ORCID: 0000-0002-8703-4451 AD - Department of Measurement and Electronics, AGH University of Krakow, 30-059 Krakow, Poland. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM MH - Humans MH - *Accelerometry/methods/instrumentation MH - *Wearable Electronic Devices MH - *Exercise/physiology MH - Male MH - Female MH - Walking/physiology MH - Running/physiology MH - Adult PMC - PMC13709298 OTO - NOTNLM OT - digital biomarkers OT - physical activity OT - population health OT - sampling frequency OT - sensor placement OT - signal processing OT - wearable accelerometry OT - wearable sensors COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 22:06 PMCR- 2026/10/21 CRDT- 2026/10/27 10:48 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:48 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - s26206652 [pii] AID - sensors-26-06652 [pii] AID - 10.3390/s26206652 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 21;26(20):6652. doi: 10.3390/s26206652. PMID- 42944514 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 21 TI - Remote Monitoring of Algorithm-Detected Compensation Cues During Long-Term Home-Based Upper-Limb Training After Stroke: A Case Series. LID - 10.3390/s26206651 [doi] LID - 6651 AB - This retrospective case series examined whether a webcam application can monitor algorithm-detected compensation cues during long-term home upper-limb training after stroke. Rules on MediaPipe landmarks, not validated in patients, trigger real-time cues for elbow flexion, shoulder elevation, lateral trunk lean, and head tilt. In a technical check on 621 clinician-labelled repetitions by healthy adults, the 90 degrees abduction rules were specific but missed most simulated compensations. Six patients with post-stroke hemiparesis, selected for long, regular records, trained at home on 33-96% of days over 3.8-9.9 months. The primary outcome, the cue rate (share of repetitions with the cue), was modelled per patient across 2277 control sessions (first daily executions of 180 degrees and 90 degrees abduction and a hand-to-mouth task). The elbow cue was most frequent in 180 degrees abduction and the shoulder cue in the hand-to-mouth task. Only four of 1s6 categorised leading-cue trajectories (all elbow cue) held in every applicable sensitivity analysis; their directions differed. Long-term markerless home monitoring of the cues was feasible in these selected patients. Cue rates reflect pace, cue priority, and programme adjustment, and clinical assessment was not repeated, so their changes are not evidence of clinical improvement or deterioration; validation in patients and controlled studies should follow. FAU - Skvortsov, Dmitry V AU - Skvortsov DV AUID- ORCID: 0000-0002-2794-4912 AD - Federal Center of Brain Research and Neurotechnologies of the Federal Medical Biological Agency (FMBA of Russia), Ostrovityanova St. 1, Bldg. 10, Moscow 117513, Russia. AD - Research and Clinical Centre of Moscow, Moscow 107031, Russia. FAU - Lobunko, Danila A AU - Lobunko DA AUID- ORCID: 0009-0009-7741-2904 AD - Federal Center of Brain Research and Neurotechnologies of the Federal Medical Biological Agency (FMBA of Russia), Ostrovityanova St. 1, Bldg. 10, Moscow 117513, Russia. FAU - Petrosyan, Vera R AU - Petrosyan VR AUID- ORCID: 0009-0001-0416-047X AD - Faculty of Information Technologies, Moscow Technical University of Communications and Informatics (MTUCI), Aviamotornaya St. 8A, Moscow 111024, Russia. FAU - Agapov, Vladislav A AU - Agapov VA AUID- ORCID: 0009-0000-8811-5547 AD - Faculty of Information Technologies, Moscow Technical University of Communications and Informatics (MTUCI), Aviamotornaya St. 8A, Moscow 111024, Russia. FAU - Pavlikov, Artem E AU - Pavlikov AE AUID- ORCID: 0009-0001-6165-7474 AD - Faculty of Information Technologies, Moscow Technical University of Communications and Informatics (MTUCI), Aviamotornaya St. 8A, Moscow 111024, Russia. FAU - Gorodnichev, Mikhail G AU - Gorodnichev MG AUID- ORCID: 0000-0003-1739-9831 AD - Faculty of Information Technologies, Moscow Technical University of Communications and Informatics (MTUCI), Aviamotornaya St. 8A, Moscow 111024, Russia. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM MH - Humans MH - *Stroke Rehabilitation/methods MH - *Upper Extremity/physiopathology/physiology MH - *Algorithms MH - Female MH - *Stroke/physiopathology MH - Aged MH - Middle Aged MH - Male MH - Cues MH - Adult MH - Retrospective Studies PMC - PMC13709264 OTO - NOTNLM OT - case series OT - compensatory movement patterns OT - home-based training OT - human pose estimation OT - markerless motion analysis OT - real-time feedback OT - remote monitoring OT - stroke rehabilitation OT - telerehabilitation OT - upper limb COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 22:06 PMCR- 2026/10/21 CRDT- 2026/10/27 10:48 PHST- 2026/09/25 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:48 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - s26206651 [pii] AID - sensors-26-06651 [pii] AID - 10.3390/s26206651 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 21;26(20):6651. doi: 10.3390/s26206651. PMID- 42944507 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 21 TI - Formation-Dependent Cardinality Concealment of Target Swarms in Multifunction Radar. LID - 10.3390/s26206644 [doi] LID - 6644 AB - Recognizing a swarm of unmanned surface vehicles does not guarantee that its physical cardinality can be recovered. This study examines how formation, viewing aspect psi, defined as the orientation of the formation relative to the radar line of sight (RLOS), and sensing resources affect these two tasks in a multifunction radar. A two-stage simulation framework evaluates group recognition against a single extended target (Stage 1) and cardinality recovery (Stage 2) using regularized linear discriminant analysis, with the aggregate nominal radar cross-section held constant. Certification is assessed using one-sided 95% confidence bounds. At N = 9 and baseline resources, group observability is certified in 12 of 35 formation-aspect conditions. For abreast formations at psi=60 composite function and 90 composite function, the group selection probability is 0.993, whereas the exact recovery probabilities are 0.108 and 0.067. This cardinality concealment, in which group observability is certified but exact recovery is not, persists over N=6,...,12 for both geometries. Coarse size information nevertheless remains, as 52-56% of estimates over N=3,...,12 lie within one target of the true value, against 28% for uniform guessing. Increasing bandwidth up to tenfold or coherent dwell up to fourfold yields no certified exact recovery. These results are specific to the evaluated simulation model and signal-processing framework and do not establish a general limitation of radar systems. FAU - Lee, Geon AU - Lee G AUID- ORCID: 0000-0001-6607-5177 AD - Department of Electrical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang 37673, Republic of Korea. FAU - Park, Seong-Ryul AU - Park SR AUID- ORCID: 0009-0001-0384-2583 AD - Department of Electrical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang 37673, Republic of Korea. FAU - Kim, Hyun-Dong AU - Kim HD AUID- ORCID: 0009-0002-8398-3868 AD - Department of Electrical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang 37673, Republic of Korea. FAU - Kim, Seung-Hwan AU - Kim SH AUID- ORCID: 0009-0004-2590-8944 AD - Department of Electrical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang 37673, Republic of Korea. FAU - Jeong, Nam-Hoon AU - Jeong NH AUID- ORCID: 0000-0003-1629-2418 AD - Agency for Defense Development, Yuseong P.O. Box 35, Daejeon 34186, Republic of Korea. FAU - Kim, Kyung-Tae AU - Kim KT AUID- ORCID: 0000-0003-1200-5282 AD - Department of Electrical Engineering, Pohang University of Science and Technology, 77 Cheongam-ro, Nam-gu, Pohang 37673, Republic of Korea. LA - eng GR - 915141301, 2025/Defense Acquisition Program Administration/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13709020 OTO - NOTNLM OT - cardinality estimation OT - multifunction radar OT - radar resource management OT - unmanned surface vehicle COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 12:53 PMCR- 2026/10/21 CRDT- 2026/10/27 10:48 PHST- 2026/09/22 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 12:53 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:48 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - s26206644 [pii] AID - sensors-26-06644 [pii] AID - 10.3390/s26206644 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 21;26(20):6644. doi: 10.3390/s26206644. PMID- 42944505 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 20 TI - Decentralising IoT-Aware Business Processes Balancing Business Risk and Energy Consumption. LID - 10.3390/s26206642 [doi] LID - 6642 AB - As Internet of Things (IoT) devices become more capable, they participate in business processes (BPs), performing process activities and making control decisions that were traditionally assigned to central systems. This creates a design-time decentralisation problem: deciding where each process element should execute given limited device resources, communication failures, and battery constraints. Existing decentralisation approaches for IoT-aware BPs mainly focus on operational criteria, such as communication overhead, energy consumption, latency, or reliability. However, they provide limited support for analysing trade-offs between competing criteria and typically do not consider the business impact of failures. This paper proposes DECR, a decision-support methodology for decentralising IoT-aware BPs by jointly considering energy consumption and business risk. DECR models risk as expected business loss by combining reliability and business value, and formulates the decentralisation problem as a bi-objective generalised assignment problem, which is solved using an adapted bi-objective simulated annealing metaheuristic. Rather than a single solution, DECR provides process stakeholders with potentially Pareto-efficient alternatives to support the selection of decentralised models according to energy constraints and risk appetite, enabling business-aligned decisions. The methodology is illustrated through an irrigation case study based on a real-world IoT prototype and further assessed through a sensitivity analysis and a computational study. FAU - Cardoso, Pedro Bandeiras AU - Cardoso PB AUID- ORCID: 0000-0001-5148-9940 AD - LASIGE, Faculdade de Ciencias, Universidade de Lisboa, 1749-016 Lisbon, Portugal. FAU - Respicio, Ana AU - Respicio A AUID- ORCID: 0000-0003-2758-7035 AD - LASIGE, Faculdade de Ciencias, Universidade de Lisboa, 1749-016 Lisbon, Portugal. FAU - Domingos, Dulce AU - Domingos D AUID- ORCID: 0000-0002-5829-2742 AD - LASIGE, Faculdade de Ciencias, Universidade de Lisboa, 1749-016 Lisbon, Portugal. LA - eng GR - UID/00408/2025/Fundacao para a Ciencia e Tecnologia/ GR - 2020.05563.BD/Fundacao para a Ciencia e Tecnologia/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13709019 OTO - NOTNLM OT - IoT OT - IoT-aware business processes OT - business process decentralisation OT - business risk OT - cyber-physical systems OT - edge computing OT - intelligent decision-support COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 12:53 PMCR- 2026/10/20 CRDT- 2026/10/27 10:48 PHST- 2026/08/05 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 12:53 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:48 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - s26206642 [pii] AID - sensors-26-06642 [pii] AID - 10.3390/s26206642 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 20;26(20):6642. doi: 10.3390/s26206642. PMID- 42944503 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 20 TI - Rolling Bearing Fault Diagnosis Based on CKEPM Encoding and Tri-CrossViT. LID - 10.3390/s26206639 [doi] LID - 6639 AB - Rolling bearings are indispensable components in mechanical equipment, and their condition directly affects the operational reliability and maintenance costs. To address the requirements of high-precision and robust bearing fault diagnosis, this paper proposes a dynamic-mapping method named CKEPM, based on Complete Ensemble Empirical Mode Decomposition (CEEMDAN), kurtosis-, energy-, and Pearson-correlation-based intrinsic mode function (IMF) selection, followed by Markov Transition Field (MTF) encoding. Furthermore, a triple-scale, cross-branch network Tri-CrossViT is constructed to hierarchically model fault-related features at different resolutions and enable cross-scale information interaction for bearing fault classification. Specifically, vibration signals are first decomposed using CEEMDAN and the fault-sensitive intrinsic mode functions are selected based on kurtosis, energy, and Pearson correlation criteria. Then, these components are encoded by the Markov Transition Field to form RGB time-frequency images. Subsequently, the proposed Tri-CrossViT processes the generated images through three parallel branches with different patch resolutions to capture fault-related information at triple scales, including localized impulsive transients, modulation-related envelope structures, and global spectral energy distributions, and a Cross-branch attention is introduced to fuse complementary fault information across scales, enabling joint learning of local impulses and global dynamic characteristics. The Tri-CrossViT achieves diagnostic accuracies of 98.87% and 99.51% on the Case Western Reserve University (CWRU) and Paderborn University (PU) datasets, respectively, realizing outstanding classification performance. The experimental results demonstrate the effectiveness of the integrated CKEPM-Tri-CrossViT framework for rolling bearing fault diagnosis under the evaluated benchmark conditions. FAU - Cui, Xingyue AU - Cui X AD - College of Computer and Information Engineering, Nanjing Tech University, Nanjing 211816, China. FAU - Bian, Yueqian AU - Bian Y AD - College of Computer and Information Engineering, Nanjing Tech University, Nanjing 211816, China. FAU - Wu, Xiaoguang AU - Wu X AD - College of Computer and Information Engineering, Nanjing Tech University, Nanjing 211816, China. FAU - Guo, Tianwen AU - Guo T AD - College of Computer and Information Engineering, Nanjing Tech University, Nanjing 211816, China. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13709581 OTO - NOTNLM OT - Complete Ensemble Empirical Mode Decomposition with Adaptive Noise OT - Markov Transition Field OT - rolling bearing fault OT - triple-scale COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 12:53 PMCR- 2026/10/20 CRDT- 2026/10/27 10:48 PHST- 2026/08/23 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 12:53 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:48 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - s26206639 [pii] AID - sensors-26-06639 [pii] AID - 10.3390/s26206639 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 20;26(20):6639. doi: 10.3390/s26206639. PMID- 42944499 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 20 TI - A Novel Cluster-Based Approach for PPG Signal Preprocessing: Motion Artifact Filtering and Ambient Light Correction. LID - 10.3390/s26206636 [doi] LID - 6636 AB - Photoplethysmography (PPG) is widely used in wearable devices, but wrist- and arm-worn recordings are sensitive to motion artifacts, ambient light, and changes in sensor-skin contact. This study presents an adaptive preprocessing pipeline that combines accelerometer-derived motion-state clustering, an ambient-light-based Contact Quality Index (CQI), and cluster-based Symlet 4 wavelet filtering. Motion states are obtained from less posture-dependent features of 10 s accelerometer windows, whereas the CQI is a heuristic indicator of ambient-light-related optical reliability, scaled from 0 to 1, that flags low-reliability segments without changing their motion label. The wavelet stage removes baseline drift, soft-thresholds the high-frequency detail bands with thresholds selected on training data, and preserves the bands that overlap the cardiac frequency range. The model was trained on 8 participants and evaluated on 27 held-out participants against a simultaneously recorded ECG. Compared with a band-pass filter and an accelerometer-referenced adaptive filter, the proposed method detected more beats (median sensitivity 1.00 vs. 0.91 and 0.92) and reduced the inter-beat-interval (IBI) error when missed beats were included (128.0 vs. 181.2 and 156.8 ms), particularly during movement, at the cost of a lower positive predictive value (0.92 vs. 1.00), whereas the reference filters yielded lower IBI error on detected beats (24.6 and 26.6 vs. 38.7 ms). The method also outperformed a fixed Daubechies 4 wavelet filter on all timing metrics. In a band-energy analysis, it reduced out-of-band energy by a median of 27.7 dB but also reduced useful-band energy by 41.0%. These results show that a lightly thresholded wavelet filter recovers more beats than fixed filters during daily-life movement, whereas the benefit of cluster-specific thresholds remains to be established in other cohorts and during higher-intensity activities. FAU - Ricci, Matteo AU - Ricci M AUID- ORCID: 0009-0003-3962-9324 AD - BioMIT.org, Electronic Engineering Department, Universitat Politecnica de Valencia, 46022 Valencia, Spain. FAU - Suarez, Daniel AU - Suarez D AUID- ORCID: 0000-0002-1273-5077 AD - Benejuzar Health Center, Orihuela Health Department, 03390 Benejuzar, Spain. FAU - Bertomeu-Gonzalez, Vicente AU - Bertomeu-Gonzalez V AUID- ORCID: 0000-0001-5309-0562 AD - Department of Clinical Medicine, Miguel Hernandez University, 03202 Elche, Spain. FAU - Martinez-Rodrigo, Arturo AU - Martinez-Rodrigo A AUID- ORCID: 0000-0003-2343-3186 AD - Research Group in Electronic, Biomedical and Telecommunication Engineering, University of Castilla-La Mancha, 16071 Cuenca, Spain. FAU - Alcaraz, Raul AU - Alcaraz R AUID- ORCID: 0000-0002-0942-3638 AD - Research Group in Electronic, Biomedical and Telecommunication Engineering, University of Castilla-La Mancha, 16071 Cuenca, Spain. FAU - Rieta, Jose J AU - Rieta JJ AUID- ORCID: 0000-0002-3364-6380 AD - BioMIT.org, Electronic Engineering Department, Universitat Politecnica de Valencia, 46022 Valencia, Spain. LA - eng GR - PID2021-123804OB-I00/Government of Spain/ GR - PID2021-128525OB-I00/Government of Spain/ GR - PID2025-73302OB-I00/Government of Spain/ GR - SBPLY/24/180225/000184/Regional Government of Castile-La Mancha/ GR - CIAICO/2023/200/Generalitat Valenciana/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13708951 OTO - NOTNLM OT - accelerometer-based clustering OT - ambient light correction OT - contact quality index OT - motion artifacts OT - photoplethysmography OT - signal preprocessing OT - wavelet denoising OT - wearable sensors COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; the collection, analysis, or interpretation of data; the writing of the manuscript; or the decision to publish the results. EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 12:53 PMCR- 2026/10/20 CRDT- 2026/10/27 10:48 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 12:53 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:48 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - s26206636 [pii] AID - sensors-26-06636 [pii] AID - 10.3390/s26206636 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 20;26(20):6636. doi: 10.3390/s26206636. PMID- 42944497 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 20 TI - MSDGFM-YOLO11n-OBB: Selective High-Resolution Difference-Guided RGB-Infrared Fusion for Aerial Oriented Vehicle Detection. LID - 10.3390/s26206634 [doi] LID - 6634 AB - Aerial vehicle detection using red-green-blue (RGB) and infrared (IR) imagery supports unmanned aerial vehicle (UAV) perception, but oriented localization remains difficult because vehicles are small, dense, arbitrarily oriented, and affected by illumination. Fusion must decide both how cross-modal differences are used and where adaptive fusion is placed. We propose MSDGFM-YOLO11n-OBB, a dual-branch oriented bounding box (OBB) detector with a Modality-Selective Difference-Guided Fusion Module (MSDGFM). Independent backbones preserve modality-specific features. MSDGFM learns sample-dependent RGB/IR weights and injects absolute cross-modal differences through a spatial gate and learnable channel-wise residual; zero initialization makes equal-weight Mean Fusion the exact starting solution. Adaptive fusion is applied only at high-resolution P3, while P4/P5 retain parameter-free Mean Fusion. On DroneVehicle Shared-label data, the three-seed results reach 0.7853 +/- 0.0027 mean average precision (mAP)@0.50 and 0.6472 +/- 0.0013 mAP@0.50:0.95, exceeding the strongest evaluated single-modal nano-scale OBB baseline by 3.88 and 6.82 percentage points. Relative to matched Mean Fusion, the gain averages 0.83 points in mAP@0.50:0.95 and is positive on all three seeds. In seed-0 capacity control, it exceeds YOLO11s-OBB (IR) by 4.32 points with 58.1% fewer parameters and 54.0% fewer FLOPs. These results support compact, selective difference-guided fusion for aerial RGB-IR OBB detection. FAU - Huang, Chao AU - Huang C AD - School of Computer Science and Technology, Zhejiang University of Science and Technology, 318 Liuhe Road, Liuxia Subdistrict, Xihu District, Hangzhou 310023, China. FAU - Xu, Zhihong AU - Xu Z AD - School of Computer Science and Technology, Zhejiang University of Science and Technology, 318 Liuhe Road, Liuxia Subdistrict, Xihu District, Hangzhou 310023, China. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13709403 OTO - NOTNLM OT - DroneVehicle OT - RGB-infrared fusion OT - YOLO11-OBB OT - aerial vehicle detection OT - difference-guided fusion OT - multispectral object detection OT - oriented object detection COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 12:53 PMCR- 2026/10/20 CRDT- 2026/10/27 10:48 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 12:53 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:48 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - s26206634 [pii] AID - sensors-26-06634 [pii] AID - 10.3390/s26206634 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 20;26(20):6634. doi: 10.3390/s26206634. PMID- 42944496 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 20 TI - All-Solid-State Ion-Selective Electrodes with Multi-Walled Carbon Nanotubes Based on Glassy Carbon for Biguanide Derivative (Chlorhexidine Digluconate). LID - 10.3390/s26206633 [doi] LID - 6633 AB - This paper presents research on the properties of solid contact electrodes with an intermediate layer of multi-walled carbon nanotubes. They were used as an intermediate layer (Solid Contact SC-ISE electrodes) and as a dispersed material in a polymer membrane (Single Piece SP-ISE electrodes). The properties of neutral macrocyclic compounds as active ion-sensitive substances in membranes, in which plasticizers with varying lipophilicity were also used, were compared. The selected optimal sensor shows a theoretical Nernstian response for the divalent chlorhexidine ion (z = 2) of approximately 29 mV decade(-1) over the linearity range of 10(-6)-10(-3) mol L(-1). A short response time of approx. 10 s, potential stability (the potential drift is several dozen muV min(-1) in a 10(-4) mol L(-1) chlorhexidine solution over 4 h), and high potential reversibility (the standard deviation in a 10(-5) mol L(-1) solution is 0.69 mV, whereas in a 10(-4) mol L(-1) solution it is 0.71 mV) are further advantages and benefits of the developed modern electrodes. In general, it can be concluded that the SC-ISE showed better potential reversibility than the SP-ISE, particularly at chlorhexidine concentrations of 10(-4) and 10(-5) mol L(-1), while also exhibiting improved resistance to water-layer formation. High selectivity was also achieved in relation to ionic substances (sodium, potassium, calcium, magnesium, acesulfame ions, and neutral substances used as excipients in chlorhexidine preparations, i.e., d-mannitol, d-sorbitol, sucralose, propylene glycol, xylitol, aspartame). The sensor with satisfactory parameters was used to determine the API content in the pharmaceutical preparation, Eludril, with a recovery of 95.9-98.6% and a relative standard deviation (RSD) ranging from 0.7 to 3.7%. FAU - Lenik, Joanna AU - Lenik J AUID- ORCID: 0000-0002-2364-4429 AD - Department of Analytical Chemistry, Institute of Chemical Sciences, Faculty of Chemistry, Maria Curie-Sklodowska University, Maria Curie-Sklodowska Square 3, 20-031 Lublin, Poland. FAU - Witkowska, Katarzyna AU - Witkowska K AD - Department of Analytical Chemistry, Institute of Chemical Sciences, Faculty of Chemistry, Maria Curie-Sklodowska University, Maria Curie-Sklodowska Square 3, 20-031 Lublin, Poland. LA - eng GR - Director's research grant, number 3/GD_INCH/2025/Director of the Institute of Chemical Sciences of Maria Curie- Sklodowska University/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13709134 OTO - NOTNLM OT - biguanide derivative OT - chlorhexidine OT - multi-walled carbon nanotubes OT - potentiometry OT - solid contact electrode COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 12:53 PMCR- 2026/10/20 CRDT- 2026/10/27 10:48 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 12:53 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:48 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - s26206633 [pii] AID - sensors-26-06633 [pii] AID - 10.3390/s26206633 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 20;26(20):6633. doi: 10.3390/s26206633. PMID- 42944494 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 20 TI - A Feasibility Study of Multimodal Optical Coherence Tomography Angiography and Elastography for Assessing Foot Microvascular Changes and Elasticity Following Repeated Mechanical Loading. LID - 10.3390/s26206631 [doi] LID - 6631 AB - The foot is regularly exposed to mechanical loading during daily activities. Repeated loading can affect both microvascular and mechanical properties of foot tissue. Optical coherence tomography angiography (OCTA) and optical coherence elastography (OCE) provide non-invasive methods for assessing these changes. This feasibility study evaluated combined OCTA and OCE imaging for measuring vascular and mechanical responses in healthy foot skin following repeated loading in 10 healthy volunteers. The dorsal and plantar regions of the foot were imaged using a portable swept-source OCT system. Baseline measurements were acquired first, followed by three repeated loading cycles (C1, C2, and C3). For each cycle, the same force was applied for 30 s. The force differed between participants according to individual tolerance and averaged approximately 25 N across the experiment. OCTA and OCE acquisition began approximately 3 s after force release to observe changes in vascular and mechanical parameters following repeated loading. In the plantar region, vessel skeleton density (VSD) showed a significant overall difference between loading conditions (p = 0.006). Young's modulus showed significant overall differences in both the dorsal and plantar regions (both p < 0.001). Relative to baseline, the dorsal Young's modulus was 22.9%, 32.3%, and 39.0% higher following C1, C2, and C3, respectively. The corresponding values in the plantar region were 18.1%, 29.2%, and 39.0% higher. Repeated-measures ANOVA identified significant loading effects for VSD, vessel area density (VAD), and Young's modulus, together with a significant region effect for VSD. These findings demonstrate the feasibility of combined OCTA and OCE imaging for assessing vascular and mechanical responses in healthy foot tissue following repeated loading. This method may support future studies of foot tissue response in populations with altered microcirculation or tissue mechanics, including people at risk of diabetic foot complications. FAU - Tang, Yu AU - Tang Y AUID- ORCID: 0009-0003-1564-9494 AD - School of Physics, Engineering and Technology, University of York, York YO10 5DD, UK. FAU - Feng, Zhengshuyi AU - Feng Z AUID- ORCID: 0009-0007-4168-6589 AD - School of Physics, Engineering and Technology, University of York, York YO10 5DD, UK. FAU - Zhang, Guangyu AU - Zhang G AUID- ORCID: 0009-0005-7290-8792 AD - School of Physics, Engineering and Technology, University of York, York YO10 5DD, UK. FAU - Gu, Jiacheng AU - Gu J AUID- ORCID: 0009-0001-0568-4486 AD - School of Physics, Engineering and Technology, University of York, York YO10 5DD, UK. FAU - Zhang, Yilong AU - Zhang Y AD - School of Physics, Engineering and Technology, University of York, York YO10 5DD, UK. FAU - Huang, Zhihong AU - Huang Z AUID- ORCID: 0000-0003-4569-5400 AD - School of Physics, Engineering and Technology, University of York, York YO10 5DD, UK. FAU - Li, Chunhui AU - Li C AUID- ORCID: 0000-0003-2186-5137 AD - Centre for Medical Engineering and Technology (CMET), School of Science and Engineering, University of Dundee, Dundee DD1 4HN, UK. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM MH - Humans MH - *Tomography, Optical Coherence/methods MH - *Elasticity Imaging Techniques/methods MH - *Foot/blood supply/diagnostic imaging/physiology MH - Feasibility Studies MH - Male MH - Adult MH - Female MH - *Angiography/methods MH - Weight-Bearing/physiology MH - *Microvessels/physiology/diagnostic imaging MH - Elasticity/physiology PMC - PMC13709094 OTO - NOTNLM OT - OCE OT - OCTA OT - foot elasticity OT - foot microcirculation OT - mechanical loading COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 22:06 PMCR- 2026/10/20 CRDT- 2026/10/27 10:48 PHST- 2026/08/19 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:48 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - s26206631 [pii] AID - sensors-26-06631 [pii] AID - 10.3390/s26206631 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 20;26(20):6631. doi: 10.3390/s26206631. PMID- 42944493 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 20 TI - STMP-UNet: Structure-Aware Target Modeling with Morphology Priors for Low-False-Alarm Detection of Small UAV Targets. LID - 10.3390/s26206630 [doi] LID - 6630 AB - Single-photon LiDAR possesses weak echo sensing capability, but random backgrounds can easily cause target response loss and fragmented false alarms. In particular, sparse return photons, background photons, and dark counts may generate stochastic responses, making weak target preservation and false-alarm suppression difficult under single-photon observations. This paper proposes STMP-UNet, which uses a structure-aware target alignment module to suppress ineffective shallow response propagation through decoder semantic constraints, and combines a morphology prior module to support the spatial continuity of coarse target-region responses. The former selectively transfers target-related shallow information under high-level semantic guidance, while the latter introduces multi-scale structural constraints to alleviate spatial fragmentation of sparse target responses. Experiments are conducted on simulated single-photon data constructed from Anti-UAV infrared sequences. A photon-counting statistical simulation is used to reproduce sparse photon responses, and an infrared-derived proximity map provides auxiliary spatial structural information. Results show that compared to Pure U-Net, STMP-UNet improves Object F1 from 0.7115 to 0.7531 and Pd from 0.6635 to 0.7074, and reduces FPPI from 0.1907 to 0.1619. Ablation and candidate-level FROC analyses further demonstrate the effectiveness of the proposed structure modeling for preserving weak target responses and improving the detection-false-alarm trade-off. FAU - Xu, Wuqiang AU - Xu W AD - State Key Laboratory of Optical Field Manipulation Science and Technology, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 610209, China. AD - University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Shen, Weihao AU - Shen W AUID- ORCID: 0009-0003-7043-1738 AD - State Key Laboratory of Optical Field Manipulation Science and Technology, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 610209, China. AD - University of Chinese Academy of Sciences, Beijing 100049, China. AD - Key Laboratory of Optical Engineering, Chinese Academy of Sciences, Chengdu 610209, China. FAU - Zhang, Jianlin AU - Zhang J AUID- ORCID: 0000-0002-5284-2942 AD - State Key Laboratory of Optical Field Manipulation Science and Technology, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 610209, China. AD - University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Xue, Rongrong AU - Xue R AD - State Key Laboratory of Optical Field Manipulation Science and Technology, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 610209, China. AD - University of Chinese Academy of Sciences, Beijing 100049, China. AD - Key Laboratory of Optical Engineering, Chinese Academy of Sciences, Chengdu 610209, China. FAU - Liu, Yang AU - Liu Y AD - State Key Laboratory of Optical Field Manipulation Science and Technology, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 610209, China. AD - University of Chinese Academy of Sciences, Beijing 100049, China. AD - Key Laboratory of Optical Engineering, Chinese Academy of Sciences, Chengdu 610209, China. FAU - Zuo, Haorui AU - Zuo H AD - State Key Laboratory of Optical Field Manipulation Science and Technology, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 610209, China. AD - University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Ma, Haotong AU - Ma H AD - State Key Laboratory of Optical Field Manipulation Science and Technology, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu 610209, China. AD - University of Chinese Academy of Sciences, Beijing 100049, China. AD - Key Laboratory of Optical Engineering, Chinese Academy of Sciences, Chengdu 610209, China. LA - eng GR - 62175243/National Natural Science Foundation of China/ GR - 12304331/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13709247 OTO - NOTNLM OT - false-alarm suppression OT - morphology prior OT - single-photon LiDAR OT - small UAV detection OT - structure-aware target alignment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 12:53 PMCR- 2026/10/20 CRDT- 2026/10/27 10:48 PHST- 2026/08/30 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 12:53 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:48 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - s26206630 [pii] AID - sensors-26-06630 [pii] AID - 10.3390/s26206630 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 20;26(20):6630. doi: 10.3390/s26206630. PMID- 42944492 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 20 TI - Machine Learning-Based Prediction of Pulmonary Function Test Outcomes Using Oscillometry in Patients with COPD. LID - 10.3390/s26206629 [doi] LID - 6629 AB - Oscillometry has been recognized as a non-invasive method for assessing respiratory conditions and is increasingly being implemented in routine clinical practice. Currently, a full pulmonary function test (PFT), which requires extensive infrastructure and trained personnel, remains the standard for comprehensive lung function assessment. Furthermore, while a full PFT can be performed by most patients with mild chronic obstructive pulmonary disease (COPD), this can be exceedingly difficult and cumbersome to complete in patients with more advanced disease. The present study sought to investigate the relationship between lung function parameters obtained by oscillometry, a non-invasive technique requiring only quiet breathing, with the parameters obtained from the more technically demanding components of a standard PFT, plethysmography and diffusing capacity, in patients with mild and very severe COPD. Through Spearman analyses, the strongest associations were observed between resistance at 5 Hz (R(5)) and the difference in resistance between 5 Hz and 19 Hz (R(5)-R(19)), by oscillometry, with residual volume (RV) to total lung capacity (TLC) ratio (RV/TLC-gas trapping) and diffusing capacity of the lung for carbon monoxide (DLco-diffusing capacity), by PFT (0.69 and -0.74, respectively). To extend the clinical applicability of oscillometry, three machine learning models suitable for small datasets, Extreme Gradient Boosting (XGBoost), Random Forest (RF), and Support Vector Regression (SVR), were implemented to predict these key PFT outcomes from oscillometry metrics and baseline demographic data alone. XGBoost achieved the best performance, with mean absolute errors of 9.7 for RV/TLC and 8.1 for DLco. In contrast, none of the models could accurately predict TLC. Feature importance analysis revealed that age enhanced RV/TLC prediction, while DLco prediction was consistently influenced by the addition of body mass index (BMI). In conclusion, oscillometry has the potential to serve as complementary to PFT for assessing otherwise tedious, resource-intensive, and often unattainable information on gas trapping and gas exchange, especially for older or frail adults with advanced disease, while still offering high accuracy. FAU - Nabavi, Seyedfakhreddin AU - Nabavi S AD - Respiratory Epidemiology and Clinical Research Unit, Centre for Outcomes Research and Evaluation, Research Institute of the McGill University Health Centre, Montreal, QC H4A 3J1, Canada. FAU - Lundblad, Lennart K A AU - Lundblad LKA AUID- ORCID: 0000-0002-1749-8242 AD - Meakins-Christie Laboratories, Research Institute of the McGill University Health Centre, Montreal, QC H4A 3J1, Canada. AD - THORASYS Thoracic Medical Systems Inc., Montreal, QC H2V 4L5, Canada. FAU - Dandurand, Ronald J AU - Dandurand RJ AUID- ORCID: 0000-0003-3235-7824 AD - Meakins-Christie Laboratories, Research Institute of the McGill University Health Centre, Montreal, QC H4A 3J1, Canada. AD - Division of Respirology, Department of Medicine, McGill University, Montreal, QC H4A 3J1, Canada. FAU - Ross, Bryan A AU - Ross BA AUID- ORCID: 0000-0001-9974-7216 AD - Respiratory Epidemiology and Clinical Research Unit, Centre for Outcomes Research and Evaluation, Research Institute of the McGill University Health Centre, Montreal, QC H4A 3J1, Canada. AD - Division of Respirology, Department of Medicine, McGill University, Montreal, QC H4A 3J1, Canada. AD - Montreal Chest Institute, McGill University Health Centre, Montreal, QC H4A 3J1, Canada. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM MH - Humans MH - *Pulmonary Disease, Chronic Obstructive/physiopathology/diagnosis MH - *Oscillometry/methods MH - *Respiratory Function Tests/methods MH - *Machine Learning MH - Male MH - Female MH - Aged MH - Middle Aged MH - Boosting Machine Learning Algorithms MH - Lung/physiopathology MH - Random Forest PMC - PMC13709072 OTO - NOTNLM OT - COPD OT - lung function test OT - machine learning OT - oscillometry OT - respiratory system COIS- L.K.A.L. is an employee of Thorasys Thoracic Medical Systems Inc., the manufacturer of the tremoflo C-100. R.J.D. has investigator-initiated grants from AstraZeneca and Boehringer Ingelheim; unrestricted grants from Thorasys; has received honoraria from the European Respiratory Society (Speaker) and the Association des Pneumologues de la Province du Quebec (Speaker); and consulting fees from Cheisi, Arrowhead Pharma and Jaegar Medical. B.A.R. reports research funding as PI from MUHC CAS, MUHC Foundation, AstraZeneca (AZ), GlaxoSmithKline (GSK), Trudell Medical International (TMI), Quebec Respiratory Health Network (QRHN), Innovation-Partnership Grant (McGill & Thorasys), MI-4, McGill Dept. of Medicine Quality Award, RI-MUHC Accelerating Clinical Trials (ACT), Fonds de Recherche du Quebec, and the Canadian Institutes of Health Research (CIHR); In-kind support for research as PI from Amazentis, Thorasys, Restech, and DiaSorin; COPD Speakerships and Moderatorship honoraria from GSK, AZ, Covis, Sanofi, Hamilton Academy of Medicine, CTS, CHEST, APPQ, AKA, and Respiplus; and consulting fees from TMI, AZ, Sanofi, GSK, and Methapharm/Chiesi. EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 22:06 PMCR- 2026/10/20 CRDT- 2026/10/27 10:48 PHST- 2026/08/20 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:48 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - s26206629 [pii] AID - sensors-26-06629 [pii] AID - 10.3390/s26206629 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 20;26(20):6629. doi: 10.3390/s26206629. PMID- 42944483 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 19 TI - DRAF-Net: Quality-Aware RGB-Skeleton Temporal Fusion for Driver Risk-Behavior Recognition. LID - 10.3390/s26206620 [doi] LID - 6620 AB - Occlusion in vehicle cabins and pose-estimation errors can reduce the reliability of skeleton information. Conventional fusion methods may propagate unreliable evidence when the RGB, joint, and bone streams produce conflicting predictions. We therefore propose DRAF-Net, a Dual-stage Risk-Aware Fusion Network. The Pose-Quality-guided Risk Bottleneck Temporal Adapter (PQR-BTA) regulates information exchange among the three streams using 12 skeleton-quality measures, prediction confidence, and cross-stream disagreement. Risk Correction Protection (RCP) then uses the baseline prediction as a reference and bounds changes to the high-risk probability and four-level classification scores. Experiments used three mutually exclusive subject splits from Drive&Act, with three random seeds per split and a shared protocol for input features, training, and validation-based model selection. Across the three subject splits, DRAF-Net achieved a high-risk AUPRC of 96.16+/-2.44%, exceeding the strongest comparison method by 3.13 percentage points. At the validation threshold targeting a FAR of approximately 5%, Recall reached 89.18+/-5.48%, an improvement of 5.82 points over the strongest comparison method. The deviations are sample standard deviations across the three split-level seed means. These results show that DRAF-Net organizes complementary RGB and skeleton evidence effectively while supporting both the ranking and detection of high-risk samples. FAU - Shen, Wei AU - Shen W AD - Key Laboratory of Advanced Manufacturing Technology for High Performance Parts, Ministry of Education, Wuhan 430070, China. AD - School of Mechanical and Electronic Engineering, Wuhan University of Technology, 122 Luoshi Road, Hongshan District, Wuhan 430070, China. FAU - Mou, Xingang AU - Mou X AUID- ORCID: 0000-0002-1265-8112 AD - Key Laboratory of Advanced Manufacturing Technology for High Performance Parts, Ministry of Education, Wuhan 430070, China. AD - School of Mechanical and Electronic Engineering, Wuhan University of Technology, 122 Luoshi Road, Hongshan District, Wuhan 430070, China. FAU - Yuan, Qun AU - Yuan Q AUID- ORCID: 0009-0004-4355-0210 AD - Key Laboratory of Advanced Manufacturing Technology for High Performance Parts, Ministry of Education, Wuhan 430070, China. AD - School of Mechanical and Electronic Engineering, Wuhan University of Technology, 122 Luoshi Road, Hongshan District, Wuhan 430070, China. FAU - Wang, Yichao AU - Wang Y AD - Key Laboratory of Advanced Manufacturing Technology for High Performance Parts, Ministry of Education, Wuhan 430070, China. AD - School of Mechanical and Electronic Engineering, Wuhan University of Technology, 122 Luoshi Road, Hongshan District, Wuhan 430070, China. FAU - Zhu, Peixuan AU - Zhu P AD - Key Laboratory of Advanced Manufacturing Technology for High Performance Parts, Ministry of Education, Wuhan 430070, China. AD - School of Mechanical and Electronic Engineering, Wuhan University of Technology, 122 Luoshi Road, Hongshan District, Wuhan 430070, China. LA - eng GR - AA23062066/Major Science and Technology Project of Guangxi/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM MH - Humans MH - Algorithms MH - *Risk-Taking MH - *Pattern Recognition, Automated/methods PMC - PMC13709200 OTO - NOTNLM OT - RGB-skeleton OT - bounded correction OT - driver behavior recognition OT - early warning OT - multimodal fusion OT - quality-aware fusion COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 22:06 PMCR- 2026/10/19 CRDT- 2026/10/27 10:48 PHST- 2026/08/29 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:48 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - s26206620 [pii] AID - sensors-26-06620 [pii] AID - 10.3390/s26206620 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 19;26(20):6620. doi: 10.3390/s26206620. PMID- 42944470 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 19 TI - Adaptive Processing of Multi-Source Drilling Sensor Signals for Coal-Rock Identification Under Limited Drilling Data: A Laboratory Case Study. LID - 10.3390/s26206607 [doi] LID - 6607 AB - Reliable coal-rock identification during drilling is challenged by limited labeled data and non-stationary, strongly coupled multi-source signals. This laboratory case study evaluates a recognition-oriented adaptive framework, IMGO-VMD-WT, that uses torque-guided improved moss growth optimization (IMGO) to select variational mode decomposition (VMD) parameters, grades intrinsic mode functions by information content, applies differentiated wavelet-threshold processing, and reconstructs the signal with residual preservation. A self-built laboratory drilling rig provided data from six material categories and eleven pressure, displacement, torque, and rotational-speed channels. Under five-fold contiguous-block validation, raw features, wavelet thresholding, fixed VMD-WT, PSO-VMD-WT, GWO-VMD-WT, and IMGO-VMD-WT achieved 90.26%, 91.94%, 92.17%, 92.13%, 92.13%, and 92.57% accuracy, respectively, with corresponding macro-F1 scores of 78.34%, 80.37%, 81.73%, 82.17%, 82.17%, and 82.22%. The exploratory paired comparison between IMGO-VMD-WT and fixed VMD-WT gave accuracy and macro-F1 differences of 0.40 and 0.49 percentage points, with exact sign-permutation p-values of 0.6875 and 0.5625 and bootstrap 95% intervals that included zero. In a controlled 10 dB noise-injection test on 12 measured torque windows, IMGO-VMD-WT reached 19.19 dB output SNR and an RMSE of 1.379. These results support decomposition-aware processing as a feasible offline strategy for limited-data laboratory drilling analysis. FAU - Liang, Bin AU - Liang B AD - School of Mechatronic Engineering, China University of Mining and Technology, No. 1 Daxue Road, Xuzhou 221116, China. AD - Xu Hai College, China University of Mining and Technology, No. 1 Jindong Road, Xuzhou 221008, China. FAU - Wei, Dong AU - Wei D AUID- ORCID: 0000-0001-8616-2040 AD - School of Mechatronic Engineering, China University of Mining and Technology, No. 1 Daxue Road, Xuzhou 221116, China. FAU - Wang, Yurou AU - Wang Y AD - School of Mechatronic Engineering, China University of Mining and Technology, No. 1 Daxue Road, Xuzhou 221116, China. FAU - Ma, Yongyuan AU - Ma Y AD - School of Mechatronic Engineering, China University of Mining and Technology, No. 1 Daxue Road, Xuzhou 221116, China. LA - eng GR - 52204179/National Natural Science Foundation of China/ GR - 2026GDS091/Special Project on Collaborative Education Mechanism of Science-Education Integration and Industry-Education Convergence for Universities in Jiangsu Province/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13708744 OTO - NOTNLM OT - coal-rock identification OT - drilling monitoring OT - limited drilling data OT - multi-source sensors OT - sensor signal processing OT - variational mode decomposition OT - wavelet thresholding COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 12:52 MHDA- 2026/10/27 12:53 PMCR- 2026/10/19 CRDT- 2026/10/27 10:48 PHST- 2026/08/15 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 12:53 [medline] PHST- 2026/10/27 12:52 [pubmed] PHST- 2026/10/27 10:48 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - s26206607 [pii] AID - sensors-26-06607 [pii] AID - 10.3390/s26206607 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 19;26(20):6607. doi: 10.3390/s26206607. PMID- 42944468 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 18 TI - Disentangling Response Magnitude and Relative MOS Sensor Fingerprints for Electronic-Nose Screening of Off-Odor in Table Olives. LID - 10.3390/s26206605 [doi] LID - 6605 AB - A multichannel metal-oxide semiconductor (MOS) electronic-nose response contains complementary information on overall response magnitude and the relative pattern among sensors. The most appropriate representation therefore depends on whether the aim is to distinguish volatile profiles, response levels, or both. This study compared three mathematically linked representations of the same six-channel NASYS S3+ response: global magnitude (M), centered log-ratio profiles (CLR), and per-sensor log-conductance ratios (LGR), which retain both components. Reference solutions varied both compound identity and concentration (four compounds, three levels, six sessions). The same framework was applied to 24 commercial cans of Californian-style black table olives preassigned by the supplier as conforming (C), mild (DS), or strong (DF) barnyard-like off-odor: 15 for calibration, six reserved before fitting, and three coded unknowns. Using the same linear discriminant analysis (LDA), CLR retained reference-compound class information better than M (balanced accuracy, BA, 0.83-0.89 vs. 50.5 Omega) whereas the reactive part collapses (+3.7 --> +0.4 Omega); the upward frequency shift and the deepening are therefore two consequences of a single reactive mechanism rather than two independent signatures. The two specimens were separated at p = 0.0095 (level) and p = 0.0080 (frequency) by the Mann-Whitney U test against a bracketed session drift of -1.3 MHz and +0.31 dB. Type A repeatability results and Type B considerations are reported following the GUM/VIM. These results establish a metrologically characterized, low-cost, non-invasive proof of concept for ATF condition monitoring, validated here on a single fresh/degraded sample pair. FAU - Duman, Mehmet AU - Duman M AUID- ORCID: 0000-0002-0831-0172 AD - Department of Electrical and Electronics Engineering, Faculty of Engineering, Duzce University, 81620 Duzce, Turkiye. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13708948 OTO - NOTNLM OT - RF-based fluid sensing OT - automotive transmission fluid degradation OT - dielectric characterization of lubricants OT - hexagonal patch array antenna OT - measurement uncertainty OT - non-contact oil condition monitoring OT - reflection-coefficient measurement OT - repeatability COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:47 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - s26206462 [pii] AID - sensors-26-06462 [pii] AID - 10.3390/s26206462 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 13;26(20):6462. doi: 10.3390/s26206462. PMID- 42944325 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 13 TI - FPGLNet: A Lightweight Frequency Prior-Gated Network for EEG Emotion Recognition. LID - 10.3390/s26206464 [doi] LID - 6464 AB - Although deep learning-based electroencephalography (EEG) emotion recognition methods have achieved promising performance, they generally depend on complex network architectures and high computational costs, which limits their deployment in resource-constrained scenarios. Meanwhile, existing approaches mainly focus on local spatiotemporal feature extraction while insufficiently exploiting global frequency-domain dependencies and dynamic information selection during feature propagation, resulting in inadequate spectral information utilization and redundant feature representations. To address these issues, this paper proposes a Frequency Prior-Gated Lightweight Network (FPGLNet) for EEG emotion recognition. First, a Mixed Aggregation Convolution (MAC) module is introduced at the input stage to jointly encode EEG spatiotemporal characteristics through temporal and spatial convolutions. Subsequently, a Fused Fourier Convolution Mixer (FFCM) module is developed to model global frequency-domain dependencies through Fourier transform and integrate multi-scale depthwise convolutions for collaborative frequency-spatial feature representation. Furthermore, a Prior-Gated Feed Forward Network (PGFN) is proposed to dynamically generate prior-guided gating weights based on feature responses, enabling adaptive selection and recalibration of discriminative features across different brain regions and temporal segments. This mechanism enhances emotion-related representations while suppressing noise and redundant information. Owing to the lightweight architecture design, FPGLNet achieves an effective balance between feature representation capability, model complexity, and computational efficiency. Experimental results demonstrate that FPGLNet achieves classification accuracies of 96.22% and 92.69% on the public SEED-IV and SEED-V datasets, respectively. These results validate the effectiveness of the proposed frequency prior-gated strategy in improving EEG feature representation while maintaining competitive recognition performance and deployment efficiency. FAU - Yuan, Yue AU - Yuan Y AD - School of Artificial Intelligence, Nanning Normal University, Nanning 530199, China. AD - Guangxi Key Laboratory of Human-Computer Interaction and Intelligent Decision-Making, Nanning Normal University, Nanning 530199, China. FAU - Bi, Yingzhou AU - Bi Y AD - School of Artificial Intelligence, Nanning Normal University, Nanning 530199, China. AD - Guangxi Key Laboratory of Human-Computer Interaction and Intelligent Decision-Making, Nanning Normal University, Nanning 530199, China. FAU - Liu, Shanrui AU - Liu S AD - College of Computer and Information Engineering, Guangxi Vocational Normal University, Nanning 530199, China. FAU - Huo, Leigang AU - Huo L AD - School of Artificial Intelligence, Nanning Normal University, Nanning 530199, China. AD - Guangxi Key Laboratory of Human-Computer Interaction and Intelligent Decision-Making, Nanning Normal University, Nanning 530199, China. LA - eng GR - 3million RMB/Nanning Normal University/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM MH - *Electroencephalography/methods MH - Humans MH - *Emotions/physiology MH - Algorithms MH - Fourier Analysis MH - Signal Processing, Computer-Assisted MH - Deep Learning PMC - PMC13709154 OTO - NOTNLM OT - EEG emotion recognition OT - dynamic gating OT - frequency domain modeling OT - lightweight network COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/13 CRDT- 2026/10/27 10:47 PHST- 2026/08/23 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - s26206464 [pii] AID - sensors-26-06464 [pii] AID - 10.3390/s26206464 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 13;26(20):6464. doi: 10.3390/s26206464. PMID- 42944324 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 13 TI - Instrumented Assessment of Static Balance Parameters and Their Association with Anthropometric Characteristics in Physiotherapy Students. LID - 10.3390/s26206461 [doi] LID - 6461 AB - Balance is a key component of postural stability, and center of pressure (CoP) parameters enable objective assessment of postural control. This study examined associations between anthropometric characteristics and CoP parameters during the Single-Leg Stance Test (SLS) in physiotherapy students. This cross-sectional observational study included 69 students from the Medical Rehabilitation undergraduate degree at the Faculty of Medicine, University of Novi Sad, Serbia, grouped according to year of study (first and fourth). Participants underwent anthropometric measurements and static balance assessment using the SLS with eyes open and closed, with CoP parameters recorded using the Vicon((R)) system and a force plate. CoP excursion in the anteroposterior (AP) and mediolateral (ML) directions and CoP mean velocity (CoP MV) were analyzed using descriptive statistics and Spearman's correlation (p < 0.05). In first-year students, CoP MV was positively correlated with body mass and BMI during right-leg stance with eyes open (r = 0.457, p < 0.05; r = 0.466, p < 0.05). In fourth-year students, CoP MV was positively correlated with body height and left lower limb length during left-leg stance with eyes open (r = 0.485, p < 0.01; r = 0.500, p < 0.01). Overall, CoP MV demonstrated the greatest number of significant associations. These findings suggest associations between anthropometric characteristics and selected static balance parameters in physiotherapy students. FAU - Vuklis, Dragana AU - Vuklis D AUID- ORCID: 0000-0002-2106-5300 AD - Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia. FAU - Krasnik, Rastislava AU - Krasnik R AUID- ORCID: 0000-0003-2702-0848 AD - Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia. AD - Institute of Child and Youth Health Care of Vojvodina, 21000 Novi Sad, Serbia. FAU - Radulovic, Milena AU - Radulovic M AUID- ORCID: 0000-0003-1739-9196 AD - Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia. FAU - Mikov, Aleksandra AU - Mikov A AUID- ORCID: 0000-0002-8919-3771 AD - Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia. AD - Institute of Child and Youth Health Care of Vojvodina, 21000 Novi Sad, Serbia. FAU - Zvekic-Svorcan, Jelena AU - Zvekic-Svorcan J AUID- ORCID: 0000-0002-3489-4351 AD - Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia. AD - Special Hospital for Rheumatic Diseases, 21000 Novi Sad, Serbia. FAU - Stanic, Milica AU - Stanic M AUID- ORCID: 0009-0008-5340-8065 AD - Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia. AD - Institute of Child and Youth Health Care of Vojvodina, 21000 Novi Sad, Serbia. FAU - Dedic Novakovic, Dajana AU - Dedic Novakovic D AD - Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia. AD - Oncology Institute of Vojvodina, 21204 Sremska Kamenica, Serbia. FAU - Nenadov Jovanovic, Natasa AU - Nenadov Jovanovic N AD - Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia. AD - Special Hospital for Rheumatic Diseases, 21000 Novi Sad, Serbia. FAU - Stupar, Jelena AU - Stupar J AD - Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia. AD - Institute of Child and Youth Health Care of Vojvodina, 21000 Novi Sad, Serbia. FAU - Heinimann, Ivana AU - Heinimann I AD - University Center of Internal Medicine, Department of Internal Medicine, Cantonal Hospital Baselland, 4101 Bruderholz, Switzerland. LA - eng PT - Journal Article PT - Observational Study DEP - 20261013 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM MH - Humans MH - *Postural Balance/physiology MH - Female MH - Male MH - *Students MH - *Anthropometry/methods MH - *Physical Therapy Modalities MH - Cross-Sectional Studies MH - Adult MH - Young Adult PMC - PMC13709065 OTO - NOTNLM OT - CoP parameters OT - Vicon motion capture system OT - center of pressure OT - force plate OT - single-leg stance test OT - static balance COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/13 CRDT- 2026/10/27 10:47 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - s26206461 [pii] AID - sensors-26-06461 [pii] AID - 10.3390/s26206461 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 13;26(20):6461. doi: 10.3390/s26206461. PMID- 42944319 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 12 TI - Estimation of Contact PPG-Derived Vascular Indices from Reconstructed rPPG Signals. LID - 10.3390/s26206458 [doi] LID - 6458 AB - Remote photoplethysmography (rPPG) enables contactless cardiovascular monitoring using conventional cameras; however, its smooth waveform morphology limits the extraction of vascular indices that depend on fine pulse-wave characteristics. Consequently, most existing rPPG studies have focused primarily on heart rate estimation rather than morphological vascular assessment. This study investigates whether reconstructed signals can support the computation of contact-PPG (cPPG)-derived vascular indices and examines how agreement varies across timing-, amplitude-, and derivative-based indices. Fiducial and second derivative photoplethysmography (SDPPG) candidates were extracted from reconstructed signals to compute the Stiffness Index (SI), Reflection Index (RI), Augmentation Index (AIx), and an exploratory Simplified Aging Index (sAI) based on the detector-returned a, b, and e candidates. The resulting indices were compared with those derived from simultaneously acquired reference cPPG within pre-segmented, temporally paired cardiac cycles with known cycle boundaries. Reference cPPG fiducial locations were not used for representative window selection or reconstructed-signal fiducial localization. Reconstruction increased the number of cycles for which the adopted detector returned the candidates required for index computation. However, a detector audit showed that strict post-notch local maxima were present in only 5.02% of reconstructed cycles, indicating that candidate availability should not be interpreted as consistent recovery of a physiologically distinct diastolic peak. Under leave-one-subject-out cross-validation across all 28 participants, subject-level agreement under the adopted computational fiducial definition was highest for SI (r=0.963, ICC(2,1) =0.926) and for the height-independent, detector-defined systolic-to-post-notch delay (r=0.909, ICC(2,1) =0.837). An exploratory strict-local-peak sensitivity analysis retained only 37 matched cycles from four participants and descriptively showed weaker agreement than the fallback-inclusive analysis. Because this small subset contained repeated cycles from only four participants, it was considered insufficient for population-level inference and was not used to support subject-level claims regarding physiological diastolic-peak recovery. RI and AIx showed only weak associations whose 95% confidence intervals included zero, and sAI showed no meaningful agreement. Under the tested additive-noise conditions, detector-defined timing measures changed relatively little between the clean and 10 dB conditions, whereas amplitude-related measures and dicrotic notch localization degraded more markedly. Additional narrowing of the retained upper cutoff below 4 Hz produced relatively small changes, but wider-band conditions could not be evaluated using the stored signals. These findings support the use of cPPG-derived vascular indices as downstream measures for technically evaluating reconstructed rPPG morphology, but they do not constitute clinical validation or establish standalone non-contact vascular assessment. FAU - Kim, SiJin AU - Kim S AUID- ORCID: 0009-0002-7638-4437 AD - Department of Sports ICT Convergence, Graduate School, Sangmyung University, Hongjimun 2-Gil 20, Jongno-Gu, Seoul 03016, Republic of Korea. FAU - Lee, Seungkeon AU - Lee S AUID- ORCID: 0000-0002-5531-028X AD - Department of AI & Informatics, Graduate School, Sangmyung University, Hongjimun 2-Gil 20, Jongno-Gu, Seoul 03016, Republic of Korea. FAU - Jeong, Saelim AU - Jeong S AUID- ORCID: 0009-0008-1488-4384 AD - Department of AI & Informatics, Graduate School, Sangmyung University, Hongjimun 2-Gil 20, Jongno-Gu, Seoul 03016, Republic of Korea. FAU - Lee, Hakjin AU - Lee H AUID- ORCID: 0009-0005-0140-3519 AD - Department of AI & Informatics, Graduate School, Sangmyung University, Hongjimun 2-Gil 20, Jongno-Gu, Seoul 03016, Republic of Korea. FAU - Choi, Kyong Keun AU - Choi KK AUID- ORCID: 0009-0004-3448-8718 AD - The Graduate School of Business Administration, Sangmyung University, Hongjimun 2-Gil 20, Jongno-Gu, Seoul 03016, Republic of Korea. FAU - Lee, Eui Chul AU - Lee EC AUID- ORCID: 0000-0001-6504-3333 AD - Department of Human-Centered AI, Sangmyung University, Hongjimun 2-Gil 20, Jongno-Gu, Seoul 03016, Republic of Korea. LA - eng GR - NRF-2024S1A5C3A02043877/National Research Foundation of Korea/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM MH - *Photoplethysmography/methods MH - Humans MH - *Signal Processing, Computer-Assisted MH - Heart Rate/physiology MH - Algorithms PMC - PMC13709017 OTO - NOTNLM OT - deep learning OT - pulse-wave analysis OT - remote photoplethysmography OT - signal processing OT - vascular biomarkers COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/12 CRDT- 2026/10/27 10:47 PHST- 2026/07/31 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - s26206458 [pii] AID - sensors-26-06458 [pii] AID - 10.3390/s26206458 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 12;26(20):6458. doi: 10.3390/s26206458. PMID- 42944315 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 12 TI - A Lightweight Multi-Sensor Feature-Level Fusion Framework for Noise-Robust Fault Diagnosis of High-Precision Milling Machines. LID - 10.3390/s26206449 [doi] LID - 6449 AB - The operational reliability of milling machines is a critical factor affecting manufacturing quality and productivity in modern industrial systems. Although multi-sensor fusion can overcome the limitations of single-sensor monitoring, many existing methods rely on computationally intensive deep learning models, which restrict their applicability in resource-constrained industrial environments. To address this issue, this paper presents a multi-sensor feature-level fusion framework for fault diagnosis in high-precision milling machines. The proposed framework integrates vibration signals from three sensing directions through signal preprocessing, multi-domain feature extraction, random-forest-based feature selection, feature-level fusion, and lightweight neural classification. Within this framework, a lightweight multilayer perceptron (MLP) is adopted as the default classifier to achieve an effective balance between diagnostic performance and computational efficiency for edge-oriented deployment. The experiments consider five machine states, comprising two non-fault operating states (No_Load and Normal) and three abnormal or fault-related states (Eccentricity, Bearing_Housing_Shim_15, and Bearing_Housing_Loose). Experimental results show that the proposed framework substantially outperforms single-sensor approaches, improving diagnostic accuracy from 74.00-86.00% to 96.00%. Comparative analysis across different classifier backbones further demonstrates that the lightweight MLP provides the most favorable efficiency-performance trade-off, while the multi-domain fusion (MDF) mechanism exhibits the strongest robustness under noisy conditions, maintaining 94.38 +/- 1.96% accuracy at 0 dB SNR and 88.88 +/- 2.53% at -10 dB SNR. These results indicate that accurate, efficient, and noise-robust milling-machine fault diagnosis can be achieved through a coordinated combination of multi-sensor feature fusion, lightweight classification, and robust fusion design, thereby providing a practical solution for intelligent industrial condition monitoring. FAU - Xu, Zhi AU - Xu Z AD - College of Automation Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China. AD - School of Mechanical and Electrical Engineering, Lianyungang Technical College, Lianyungang 222000, China. FAU - Lu, Ningyun AU - Lu N AD - College of Automation Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China. FAU - Jin, Liguo AU - Jin L AUID- ORCID: 0009-0001-1986-1180 AD - College of Automation Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China. LA - eng GR - BK20243045/Jiangsu Province Science and Technology Department/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13709204 OTO - NOTNLM OT - edge computing OT - feature-level fusion OT - lightweight neural network OT - milling-machine fault diagnosis OT - multi-sensor fusion COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:47 PHST- 2026/07/25 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - s26206449 [pii] AID - sensors-26-06449 [pii] AID - 10.3390/s26206449 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 12;26(20):6449. doi: 10.3390/s26206449. PMID- 42944314 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 12 TI - Evaluation of Machine Vision-Based Automated Detection of Freeway Incidents. LID - 10.3390/s26206451 [doi] LID - 6451 AB - Automatic incident detection (AID) can significantly decrease the time to detect incidents and better identify incident locations to allow faster response. The deployment of traffic surveillance cameras has motivated transportation agencies to investigate computer vision technologies for AID using live video streams from these cameras. This paper evaluated three commercially available computer vision-based AID systems. The evaluated AID systems were able to use video streams from six existing Pan-Tilt-Zoom (PTZ) surveillance cameras for incident detection when these cameras were on predefined presets. The three systems with the selected settings performed differently. For example, the system with the highest number of true stopped vehicle incident detections also had a relatively high number of false alerts. The AID system accuracy also varied by study site, likely due to traffic patterns, roadway geometry, and camera angles. Two major factors contributed to false alerts: stop-and-go traffic during non-incident conditions and other highway objects, including light reflection and glare, that were falsely detected as incidents. The three systems also detected additional incidents not reported in existing incident management databases, but these were mainly shoulder incidents and work zone vehicles. FAU - Jeenprasom, Chonlachart AU - Jeenprasom C AUID- ORCID: 0009-0006-2543-4616 AD - Department of Civil and Environmental Engineering, Florida International University, 10555 West Flagler Street, EC 3730, Miami, FL 33174, USA. FAU - Sirikanchittavon, Tanchanok AU - Sirikanchittavon T AUID- ORCID: 0009-0004-9829-4584 AD - Department of Civil and Environmental Engineering, Florida International University, 10555 West Flagler Street, EC 3730, Miami, FL 33174, USA. FAU - Hadi, Mohammed AU - Hadi M AUID- ORCID: 0000-0003-2233-8283 AD - Department of Civil and Environmental Engineering, Florida International University, 10555 West Flagler Street, EC 3730, Miami, FL 33174, USA. FAU - Wang, Zhenyu AU - Wang Z AUID- ORCID: 0000-0003-1185-7600 AD - Center for Urban Transportation Research, University of South Florida, 4202 E Flowe Ave, CUT100, Tampa, FL 33620, USA. LA - eng GR - BED25 9777-13/Florida Department of Transportation/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13709278 OTO - NOTNLM OT - automated incident detection OT - computer vision OT - freeway incidents COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:47 PHST- 2026/07/30 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - s26206451 [pii] AID - sensors-26-06451 [pii] AID - 10.3390/s26206451 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 12;26(20):6451. doi: 10.3390/s26206451. PMID- 42944313 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 12 TI - Fault-Diagnosis-Aware Adaptive Policy Switching for Robust Map-Free Mobile Robot Navigation Under Sensor and Control Degradation. LID - 10.3390/s26206452 [doi] LID - 6452 AB - Heterogeneous sensor and control degradation can destabilize direct sensor-to-action navigation. This study presents a fault-diagnosis-aware adaptive policy-switching framework that combines a calibrated seven-class temporal health-state classifier with hysteretic routing among nominal, robust-fallback, and six fault-specialized proximal policy optimization (PPO) policies. Unlike monolithic robust reinforcement learning, the architecture makes the inferred degradation mode an explicit and auditable control variable and separates health-state inference from behavior selection. Across a large held-out simulation evaluation with three independently trained policy seeds, diagnosed switching improved the central severity-0.55 static benchmark and within-episode transition outcomes relative to robust PPO, while the broader severity sweep showed regime-dependent behavior. Diagnosed switching also retained an aggregate advantage under interaction-matched robust training. Mechanism controls showed that the gain depended on fault-specific specialist competence and correct fault-to-specialist correspondence rather than policy-bank capacity alone. Single-thread inference remained well below the 200 ms control interval. Within the evaluated simulation scope, the results support explicit online diagnosis and behaviorally matched policy selection as a practical strategy for robust map-free navigation under heterogeneous degradation. FAU - Yilmaz, Taner AU - Yilmaz T AUID- ORCID: 0000-0002-1721-9071 AD - Faculty of Technology, Department of Mechatronics Engineering, Firat University, Elazig 23119, Turkiye. FAU - Aydogmus, Omur AU - Aydogmus O AUID- ORCID: 0000-0001-8142-1146 AD - Faculty of Technology, Department of Mechatronics Engineering, Firat University, Elazig 23119, Turkiye. LA - eng GR - TEKF.25.28/Scientific Research Projects Coordination Unit of Firat University (FUBAP)/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13708949 OTO - NOTNLM OT - LiDAR degradation OT - adaptive control OT - fault diagnosis OT - fault-tolerant control OT - mobile-robot navigation OT - policy switching OT - proximal policy optimization OT - reinforcement learning COIS- The authors declare no conflicts of interest. The funder had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:47 PHST- 2026/08/17 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - s26206452 [pii] AID - sensors-26-06452 [pii] AID - 10.3390/s26206452 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 12;26(20):6452. doi: 10.3390/s26206452. PMID- 42944308 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 12 TI - Distributed Non-Invasive Electrical Monitoring and Short-Term Aggregate Load Forecasting for Wastewater Pumping Stations Using Custom Sensor Nodes and Neural Networks. LID - 10.3390/s26206445 [doi] LID - 6445 AB - Continuous electrical data can support both monitoring and short-term forecasting in distributed infrastructures. This study presents an end-to-end framework for non-invasive current monitoring and forecasting of the platform-derived aggregate load of wastewater pumping stations. The deployed architecture combines SCT current sensors, Arduino Uno acquisition, a custom EasyEDA-designed electronic interface, Raspberry Pi-based storage and communication, and remote data aggregation. Fourteen stations comprising 23 three-phase pumps are covered by 69 sensing channels. The available dataset comprises 720 consecutive hourly observations. A rolling-origin protocol uses 18 days for initial fitting, five daily origins for hyperparameter selection, and seven held-out daily origins for testing, yielding 168 out-of-sample predictions. A seasonal-naive benchmark was compared with a linear autoregressive model, a multilayer perceptron (MLP), and an RBF-GRNN. None of the three fitted models showed a statistically conclusive reduction in MAE relative to the seasonal-naive benchmark, because all paired block-bootstrap 95% confidence intervals for the MAE differences included zero. Descriptively, among the four prespecified models, the MLP achieved the lowest MAE (0.441 kW) and MAPE (3.266%), whereas the RBF-GRNN achieved the lowest RMSE (0.525 kW) and the highest R(2) (0.918). An exploratory post hoc LSTM analysis yielded an MAE of 0.424 kW but a higher RMSE (0.637 kW) and maximum error (2.959 kW); it likewise did not demonstrate a statistically conclusive advantage over either the MLP or the seasonal-naive benchmark. The results demonstrate a modular sensing-to-forecasting workflow while showing that the observed differences among models require validation over longer monitoring periods. FAU - Lagana, Filippo AU - Lagana F AUID- ORCID: 0000-0001-6846-1784 AD - Laboratory of Biomedical Applications Technologies and Sensors (BATS), Department of Health Science, "Magna Graecia" University, 88100 Catanzaro, Italy. LA - eng PT - Journal Article DEP - 20261012 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13708763 OTO - NOTNLM OT - forecasting OT - monitoring OT - pumping OT - sensors OT - wastewater COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:47 PHST- 2026/08/23 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - s26206445 [pii] AID - sensors-26-06445 [pii] AID - 10.3390/s26206445 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 12;26(20):6445. doi: 10.3390/s26206445. PMID- 42944306 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 12 TI - Physics-Guided Multi-View Transformer with Block Attention Residuals for Cross-Condition Railway Bearing Fault Diagnosis. LID - 10.3390/s26206439 [doi] LID - 6439 AB - Railway bearings experience speed- and load-dependent distribution shifts that complicate fault diagnosis. This study proposes a Physics-Guided Multi-View Transformer with Block Attention Residuals (PGMV-BAR). Characteristic-order prior tokens sequentially query envelope-order and time-frequency features and remain in the fusion sequence with a separate condition token. A two-stage strategy combines masked reconstruction, contrastive consistency, prior alignment, and supervised fine-tuning. Evaluation follows a source-only cross-condition protocol. Beijing Jiaotong University-Rail Autonomous Operations (BJTU-RAO) includes nine combinations of motor frequency (20, 40, and 60 Hz) and lateral load (-10, 0, and +10 kN), with a constant vertical load of 10 kN. All 36 unordered pairs are evaluated as held-out test conditions, with the remaining seven conditions supplying source training and validation in each task. The L dataset is evaluated through six leave-one-condition-out tasks formed by 1200, 1500, and 1800 r/min at 0% and 10% rated torque, using the other five conditions as sources. Held-out-condition samples are excluded from pretraining, fine-tuning, and checkpoint selection. PGMV-BAR achieves a mean accuracy of 97.27% across the 36 BJTU-RAO tasks (96.05-99.21%) and 95.33% across the L tasks. These results support cross-condition diagnosis within the evaluated datasets. FAU - Liang, Junjie AU - Liang J AD - State Key Laboratory of Advanced Rail Autonomous Operation, Beijing Jiaotong University, Beijing 100044, China. FAU - Cai, Guoqiang AU - Cai G AUID- ORCID: 0000-0003-2773-834X AD - State Key Laboratory of Advanced Rail Autonomous Operation, Beijing Jiaotong University, Beijing 100044, China. FAU - Zhang, Zhengming AU - Zhang Z AD - State Key Laboratory of Advanced Rail Autonomous Operation, Beijing Jiaotong University, Beijing 100044, China. FAU - Lin, Shuqin AU - Lin S AD - State Key Laboratory of Advanced Rail Autonomous Operation, Beijing Jiaotong University, Beijing 100044, China. FAU - Song, Yuxiao AU - Song Y AD - State Key Laboratory of Advanced Rail Autonomous Operation, Beijing Jiaotong University, Beijing 100044, China. FAU - Wu, Zihao AU - Wu Z AD - State Key Laboratory of Advanced Rail Autonomous Operation, Beijing Jiaotong University, Beijing 100044, China. LA - eng GR - 2022YFB4301204/National Key Research and Development Program of China/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13708857 OTO - NOTNLM OT - cross-condition OT - fault diagnosis OT - physics-guided multi-view transformer OT - railway bearing COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:47 PHST- 2026/08/20 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - s26206439 [pii] AID - sensors-26-06439 [pii] AID - 10.3390/s26206439 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 12;26(20):6439. doi: 10.3390/s26206439. PMID- 42944296 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 12 TI - A Dual-Modal Hybrid Marker for Automatic Registration and Coordinate Hand-Over in C-Arm CT-Guided Spinal Navigation. LID - 10.3390/s26206433 [doi] LID - 6433 AB - An asymmetric hybrid registration frame (RF) integrates radiopaque and optical markers to automate computed tomography (CT) registration and coordinate hand-over to a dynamic reference frame (DRF). We compared automatic and manual fiducial selection using three fixed CT datasets of a rigid sawbone phantom. Ten operators performed five manual registrations per CT, alongside one automatic benchmark per CT. Both methods used the same five registration fiducials, rigid registration procedure, and three evaluation centers excluded from transform estimation. Target registration error (TRE) was the root mean square distance between transformed design centers and CT-detected centers, without probe contact. Across equally weighted CT datasets, mean TRE was 0.538 mm for automatic and 1.565 mm for manual registration. The manual-minus-automatic difference was 1.027 mm (95% operator-bootstrap confidence interval, 0.951-1.148 mm). Mean registration processing times were 2.817 and 22.589 s, respectively. Under these common evaluation conditions, automatic fiducial detection reduced on-frame center discrepancy and registration time. These findings support the integrated registration and handover workflow as a basis for further validation at independent anatomical targets. FAU - Kim, Taeho AU - Kim T AUID- ORCID: 0000-0003-2037-0829 AD - Department of Electrical, Electronic, and Computer Engineering, University of Ulsan, Ulsan 44610, Republic of Korea. FAU - Kim, Sungmin AU - Kim S AUID- ORCID: 0000-0003-2859-4210 AD - School of Mechanical Engineering, University of Ulsan, Ulsan 44610, Republic of Korea. LA - eng GR - RS-2024-00507664/Ministry of Trade, Industry and Energy/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM MH - *Tomography, X-Ray Computed/methods MH - Humans MH - Fiducial Markers MH - Phantoms, Imaging MH - *Spine/diagnostic imaging/surgery MH - Image Processing, Computer-Assisted/methods MH - *Surgery, Computer-Assisted/methods MH - Algorithms PMC - PMC13709288 OTO - NOTNLM OT - C-arm CT OT - automatic registration OT - coordinate handover OT - hybrid marker OT - spinal navigation OT - target registration error OT - target-proximal re-registration COIS- The authors declare no conflicts of interest; the funders had no role in study design, data collection, analysis or interpretation, manuscript preparation, or decision to publish. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/12 CRDT- 2026/10/27 10:47 PHST- 2026/09/12 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - s26206433 [pii] AID - sensors-26-06433 [pii] AID - 10.3390/s26206433 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 12;26(20):6433. doi: 10.3390/s26206433. PMID- 42944294 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 12 TI - Coordinated Regulation of Orchard Variable-Rate Spraying: From Canopy Perception to Actuation-A Review. LID - 10.3390/s26206431 [doi] LID - 6431 AB - Orchard variable-rate spraying increasingly depends on the coordinated conversion of heterogeneous perception outputs into spatially and temporally matched actuator commands, yet the evidence linking sensing improvements to measurable spray-performance gains remains fragmented. Rather than reviewing canopy perception technologies in isolation, this review focuses on the complete perception-to-actuation chain for coordinated orchard spraying and identifies which perception outputs are sufficiently actionable for regulation of liquid flow, airflow, nozzle timing, and spray trajectory. A structured narrative review of the peer-reviewed literature published up to August 2026 was organized by perception object, sensing modality, platform type, orchard system, and control relevance; representative studies were retained when they reported a clear linkage between sensed information and spraying decisions or provided transferable evidence for that linkage. Four perception-information classes are distinguished: canopy geometric profile, internal canopy structure, crop/plant-protection status, and spatial-geographic/operational context. LiDAR, RGB/RGB-D and binocular vision, multispectral/hyperspectral sensing, positioning, and multimodal fusion are compared according to the information they provide and their suitability for real-time control. Cross-study synthesis shows that the strongest evidence currently concerns canopy-volume- or density-based liquid-flow-rate regulation, while robust mappings for airflow demand, disease-severity-based dosage, and cross-season transfer remain less mature. Reported benefits include substantial pesticide savings in selected field or prototype studies, but the evidence is heterogeneous and often species-, platform-, and scenario-specific. The central research need is, therefore, not higher perception accuracy alone, but physically interpretable, latency-aware, spatially registered mappings from heterogeneous perception outputs to executable nozzle, liquid-flow, airflow, and trajectory commands. Concrete priorities include standardized structural descriptors, end-to-end delay compensation, transferable dose models, and benchmark field protocols for closed-loop validation. FAU - Dai, Shiqun AU - Dai S AUID- ORCID: 0009-0006-4416-4252 AD - School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China. AD - Key Laboratory of Plant Protection Engineering, Ministry of Agriculture and Rural Affairs, Jiangsu University, Zhenjiang 212013, China. FAU - Tu, Dengjun AU - Tu D AD - School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China. FAU - Fan, Xurui AU - Fan X AD - School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China. FAU - Wang, Ming AU - Wang M AD - School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China. FAU - Zhang, Zhiyang AU - Zhang Z AD - School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China. FAU - Zhu, Wenrui AU - Zhu W AD - School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China. FAU - Jiang, Li AU - Jiang L AUID- ORCID: 0009-0006-5859-3322 AD - School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China. AD - Key Laboratory of Plant Protection Engineering, Ministry of Agriculture and Rural Affairs, Jiangsu University, Zhenjiang 212013, China. FAU - Zhou, Jianfei AU - Zhou J AD - School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China. AD - Key Laboratory of Plant Protection Engineering, Ministry of Agriculture and Rural Affairs, Jiangsu University, Zhenjiang 212013, China. FAU - Jia, Weidong AU - Jia W AD - School of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, China. AD - Key Laboratory of Plant Protection Engineering, Ministry of Agriculture and Rural Affairs, Jiangsu University, Zhenjiang 212013, China. LA - eng GR - BF2025313-06/Jiangsu Province Science and Technology Department/ GR - PAPD-2023-87/Jiangsu University/ PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 RN - 0 (Pesticides) SB - IM MH - *Agriculture/methods MH - Pesticides MH - Crops, Agricultural PMC - PMC13709032 OTO - NOTNLM OT - LiDAR OT - machine vision OT - multispectral sensing OT - orchard mapping OT - precision pesticide application OT - prescription map OT - sensor-actuator mapping OT - spatial registration COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/12 CRDT- 2026/10/27 10:47 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - s26206431 [pii] AID - sensors-26-06431 [pii] AID - 10.3390/s26206431 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 12;26(20):6431. doi: 10.3390/s26206431. PMID- 42944292 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 12 TI - Field Evaluation of a Portable Multi-Sensor Platform for Characterizing Inter-Individual Variability in Exhaled Breath Responses. LID - 10.3390/s26206429 [doi] LID - 6429 AB - Portable breath-sensing platforms offer a practical approach for collecting physiological and environmental information outside controlled laboratory settings, but their interpretation is complicated by inter-individual variability and sensor-related effects. This pilot study evaluated the BreathLogix VOC Series prototype, a portable multi-sensor platform incorporating gas-sensitive, carbon-dioxide, temperature, and environmental channels, during routine collegiate athletic activities. A total of 421 breath recordings were obtained from 209 participant ID codes, including 175 pre-exercise and 246 post-exercise recordings. Continuous sensor signals were converted into aggregated sample-level features and analyzed using descriptive statistics, interquartile-range comparisons, Pearson correlation analysis, and K-means clustering. Group-level median responses remained relatively stable across exercise conditions, whereas response variability changed more noticeably. Humidity showed the largest post-exercise increase in dispersion, followed by the hydrogen- and methane-sensitive channels, while the acetone-sensitive response showed reduced variability. Correlation analysis identified strong relationships among selected channels, consistent with possible cross-sensitivity and environmental influence. Clustering revealed two overlapping sensor-derived breath-signature groups primarily separated by acetone-sensitive response; a sex difference in cluster composition was not retained when each participant was counted once. These findings demonstrate the feasibility of field-based multi-channel breath acquisition and highlight the value of variability-centered analysis. Further analytical validation, calibration assessment, and longitudinal reproducibility testing are required. FAU - Olla, Phillip AU - Olla P AD - Health Services Administration, College of Health Professions, University of Detroit Mercy, Detroit, MI 48221, USA. AD - Center for Augmenting Intelligence, University of Detroit Mercy, Detroit, MI 48221, USA. FAU - Shannan, Sara AU - Shannan S AD - Department of Electrical & Computer Engineering & Computer Science, University of Detroit Mercy, Detroit, MI 48221, USA. FAU - Maleki, Mina AU - Maleki M AD - Department of Electrical & Computer Engineering & Computer Science, University of Detroit Mercy, Detroit, MI 48221, USA. FAU - Travo, Ava AU - Travo A AUID- ORCID: 0009-0007-3750-9221 AD - London Health Sciences Centre, University of Western Ontario, London, ON N6A 3K7, Canada. FAU - Paknahad, Mohammad AU - Paknahad M AD - Cannabix Technologies Inc., 501-3292 Production Way, Burnaby, BC V5A 4R4, Canada. FAU - Bandi, Ava AU - Bandi A AD - Center for Augmenting Intelligence, University of Detroit Mercy, Detroit, MI 48221, USA. AD - Department of Electrical & Computer Engineering & Computer Science, University of Detroit Mercy, Detroit, MI 48221, USA. FAU - Favor, Nina AU - Favor N AD - Health Services Administration, College of Health Professions, University of Detroit Mercy, Detroit, MI 48221, USA. LA - eng GR - Titan Innovation Award/University of Detroit Mercy/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 RN - 142M471B3J (Carbon Dioxide) RN - 0 (Volatile Organic Compounds) SB - IM MH - Humans MH - Breath Tests/instrumentation/methods MH - Male MH - Female MH - *Exhalation/physiology MH - Adult MH - Pilot Projects MH - Carbon Dioxide/analysis MH - Volatile Organic Compounds/analysis MH - Young Adult MH - Exercise/physiology PMC - PMC13709153 OTO - NOTNLM OT - electronic nose OT - inter-individual variability OT - metal oxide semiconductor sensor OT - multi-sensor array OT - portable breath sensor COIS- P.O. serves on the Board of Directors of Cannabix Technologies Inc., the manufacturer of the BreathLogix device used in this study. M.P. is employed by Cannabix Technologies Inc. The BreathLogix device was provided to the University of Detroit Mercy by Cannabix Technologies Inc. at no charge. All other authors declare no conflicts of interest. The funders had no role in the collection, analysis, or interpretation of data. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/12 CRDT- 2026/10/27 10:47 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - s26206429 [pii] AID - sensors-26-06429 [pii] AID - 10.3390/s26206429 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 12;26(20):6429. doi: 10.3390/s26206429. PMID- 42944290 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 11 TI - Cost-Aware Online Switching of Four-Qubit Probe States for Quantum Magnetometry Under Non-Stationary Noise. LID - 10.3390/s26206428 [doi] LID - 6428 AB - Quantum magnetometry can be enhanced through the use of entangled probe states, yet the optimal probe selection depends greatly on the noise, readout errors, and costs involved in preparation. An analysis is given of a simulation of a four-qubit magnetic field estimation experiment where the optimal probe state is adaptively chosen via an online controller that changes between product and GHZ probes using delayed and self-consistent feedback without knowing the underlying noise model. The performance of the controller is compared to that of a validation-selected fixed probe state strategy, a hindsight fixed probe strategy, a fixed product probe strategy, a fixed GHZ probe strategy, a fixed graph-state probe strategy, and an oracle-based dynamic probe strategy in eight stationary and non-stationary noise environments. In the actual experiment, 20 random seeds, 1200 timesteps per noise scenario, and 2000 measurement shots per timestep in the case of local dephasing with readout error are used. The sliding-window controller yields an overall RMSE of 0.015542 versus 0.017305 for the validation-selected fixed probe state, 0.017330 for the fixed product, 0.017821 for fixed GHZ, and 0.061184 for the fixed graph state. A paired permutation test on 160 trial cases gives the mean RMSE difference of -0.001763 versus the validation-selected fixed probe state with p=9.99x10-4. The adaptive algorithm improves performance in seven out of eight noise environments but still does not do well enough in the constant high-noise scenario at 5.4% worse than the global fixed baseline in the constant-high-noise scenario. FAU - Diwakar, Diwakar AU - Diwakar D AUID- ORCID: 0000-0002-4272-4467 AD - Quantum Technology Institute & Department of Computer Science and Engineering, SRM University-AP, Amaravati 522240, Andhra Pradesh, India. FAU - Sambhaje, Varsha Santosh AU - Sambhaje VS AUID- ORCID: 0000-0002-7163-8613 AD - Quantum Technology Institute & Department of Computer Science and Engineering, SRM University-AP, Amaravati 522240, Andhra Pradesh, India. LA - eng GR - SRM University, Andhra Pradesh/ PT - Journal Article DEP - 20261011 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13709144 OTO - NOTNLM OT - GHZ states OT - adaptive control OT - finite-shot simulation OT - non-stationary noise OT - online learning OT - quantum magnetometry OT - quantum sensing COIS- The authors declare no conflicts of interest. The funder had no role in the design of the study; data generation, analysis, or interpretation; preparation of the manuscript; or decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/11 CRDT- 2026/10/27 10:47 PHST- 2026/08/14 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - s26206428 [pii] AID - sensors-26-06428 [pii] AID - 10.3390/s26206428 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 11;26(20):6428. doi: 10.3390/s26206428. PMID- 42944283 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 11 TI - Comparative Analysis of Methods for Improving the Classification Accuracy of Morphologically Similar Pollen Grain Classes on the CPD-1 Dataset. LID - 10.3390/s26206422 [doi] LID - 6422 AB - Automatic classification of pollen grains in microscopic images supports melissopalynology and honey quality control but is complicated by class imbalance and morphologically similar classes. We evaluated factors affecting classification on the Cretan Pollen Dataset v1 (CPD-1; 20 classes, 4025 images) with YOLOv8s-cls, YOLO11s-cls, YOLO26s-cls, EfficientNet-B3 and a reproduced RCANet. Each configuration was trained with five seeds; hyperparameters and ensembles were selected on validation data only. Augmentation improved the macro F1-score by about 1.2 percentage points; a size-matched unbalanced control showed that this gain came from the larger training set, not from class balancing. The most difficult class, Ceratonia, was misclassified mainly as Vitis and Olea; its F1-score fell from 0.878 to 0.778 when its original training images were reduced from 26 to 10. Focal Loss and Squeeze-and-Excitation blocks did not improve Ceratonia reproducibly (changes in its F1-score from -0.03 to +0.03, not significant after Holm correction), and the YOLO architectures could not be ranked reliably. An ensemble of EfficientNet-B3 and YOLO models selected on validation data outperformed the best single model in all seeds (macro F1-score 0.9720 vs. 0.9631). Several single-run effects were not reproduced with multiple seeds, underlining the need for multi-seed evaluation and validation-only selection. FAU - Mukhamadiyev, Abdinabi AU - Mukhamadiyev A AUID- ORCID: 0000-0002-1438-0628 AD - Department of Computer Engineering, Gachon University, Sujeong-gu, Seongnam-si 13120, Republic of Korea. FAU - Safarov, Rustam AU - Safarov R AUID- ORCID: 0000-0002-6092-2408 AD - Department of Systematic and Practical Programming, Tashkent University of Information Technologies named after Muhammad al-Khwarizmi, Tashkent 100084, Uzbekistan. AD - Department of Management Theory and Information Security, Samarkand State University named after Sharof Rashidov, Samarkand 140100, Uzbekistan. FAU - Jumanov, Isroil AU - Jumanov I AUID- ORCID: 0000-0002-4259-6194 AD - Department of Artificial Intelligence and Information Systems, Samarkand State University named after Sharof Rashidov, Samarkand 140100, Uzbekistan. FAU - Kholmonov, Sunatillo AU - Kholmonov S AD - Department of Artificial Intelligence and Information Systems, Samarkand State University named after Sharof Rashidov, Samarkand 140100, Uzbekistan. FAU - Khujakulov, Toshtemir AU - Khujakulov T AD - Department of Computer Engineering, Tashkent University of Applied Sciences, Tashkent 100125, Uzbekistan. FAU - Khasanov, Doston AU - Khasanov D AUID- ORCID: 0000-0001-5285-3775 AD - Department of Data Communication Networks and Systems, Tashkent University of Information Technologies, Tashkent 100200, Uzbekistan. FAU - Khujamatov, Ergashevich Halimjon AU - Khujamatov EH AUID- ORCID: 0000-0001-5206-635X AD - Department of Data Communication Networks and Systems, Tashkent University of Information Technologies, Tashkent 100200, Uzbekistan. AD - Department of Digital Pedagogy and Media Education Development, Republican Scientific and Methodological Center for the Development of Education of the Republic of Uzbekistan, Tashkent 100180, Uzbekistan. AD - Department of Applied Informatics and Information Security, Osh State University, Osh 723500, Kyrgyzstan. AD - Department of Information Systems and Technologies, Fergana State Technical University, Fergana 150100, Uzbekistan. AD - Department of Computer and Software Engineering, Jizzakh Polytechnic Institute, Jizzakh 130100, Uzbekistan. FAU - Jeon, Heung Seok AU - Jeon HS AD - Department of Computer Engineering, AI Convergence Research Center, Konkuk University, Chungju 27478, Republic of Korea. FAU - Lee, Cheolwon AU - Lee C AUID- ORCID: 0000-0002-2778-2562 AD - Department of Computer Engineering, AI Convergence Research Center, Konkuk University, Chungju 27478, Republic of Korea. LA - eng PT - Comparative Study PT - Journal Article DEP - 20261011 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM MH - *Pollen/classification MH - Classification Algorithms MH - Image Processing, Computer-Assisted/methods MH - Algorithms PMC - PMC13708861 OTO - NOTNLM OT - Cretan Pollen Dataset OT - EfficientNet OT - Focal Loss OT - Squeeze-and-Excitation OT - YOLO OT - class imbalance OT - data augmentation OT - model ensembling OT - pollen grain classification OT - reproducibility COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analysis, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/11 CRDT- 2026/10/27 10:47 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - s26206422 [pii] AID - sensors-26-06422 [pii] AID - 10.3390/s26206422 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 11;26(20):6422. doi: 10.3390/s26206422. PMID- 42944279 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 11 TI - Mode-Selective Nonlinearity in a Monolithic Slitted Piezoelectric Energy Harvester: Symmetry-Controlled Internal Resonance, Saturation, and Differential Bistability. LID - 10.3390/s26206416 [doi] LID - 6416 AB - Nonlinear elements in vibration energy harvesters conventionally act on coordinates that every structural mode shares, so added functionality detunes the power-carrying resonance. We analyse a different placement: a permanent-magnet pair mounted inside the slit of a monolithic slitted piezoelectric cantilever, acting only on the difference in the prong deflections. Because this coordinate is odd under the device mirror symmetry, an exact selectivity theorem holds: at equal tip masses, the element is invisible to the symmetric mode family at every order in amplitude, while a rank-one lever places the antisymmetric resonance across 33-60 Hz. Deliberate mass mistuning mu then opens a gated 2:1 internal resonance (coupling asymptotically proportional, variant mu(2), activation threshold proportional, variant mu(-3)), verified against direct integration of the full cubic model to 0.1-2% and cross-checked by an independent finite element model. The two regimes trade off: at the mistuned design point, the first resonance shifts by 5.9%, and thresholdless transfer syphons 38% of its matched-load power at 0.05 g. The device serves a 43.3 Hz line at 16.6-20.1 muW, where the symmetric platform delivers 0.15-0.21 muW, integrates 448 vs. 354 muW⋅Hz over the band (1.27x), with unloaded/matched activation thresholds 0.017 g/0.079 g and an optimal load fraction rho( small star, filled) approximately 0.34. FAU - Almokmesh, Saad F AU - Almokmesh SF AD - Department of Mechanical Power and Refrigeration Technology, College of Technological Studies, Public Authority for Applied Education and Training (PAAET), Kuwait City 70654, Kuwait. FAU - Alzuwayer, Bashar B AU - Alzuwayer BB AD - Department of Automotive Mechanics and Marine Engineering Technology, College of Technological Studies, Public Authority for Applied Education and Training (PAAET), Kuwait City 70654, Kuwait. LA - eng PT - Journal Article DEP - 20261011 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13709030 OTO - NOTNLM OT - bistability OT - internal resonance OT - mode selectivity OT - piezoelectric energy harvesting OT - saturation OT - slitted cantilever OT - symmetry breaking COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/11 CRDT- 2026/10/27 10:47 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - s26206416 [pii] AID - sensors-26-06416 [pii] AID - 10.3390/s26206416 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 11;26(20):6416. doi: 10.3390/s26206416. PMID- 42944276 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 10 TI - DSC: Containment-Aware Instance Consolidation for Ultra-High-Resolution Railway Signal-Layout Image Analysis. LID - 10.3390/s26206413 [doi] LID - 6413 AB - High-resolution engineering drawings are commonly analyzed in overlapping tiles, which can produce several predictions showing different extents of the same object. Such observations may have low intersection-over-union despite substantial foreground containment. We propose document-space consolidation (DSC), which projects local instance predictions into drawing coordinates and applies class-wise box non-maximum suppression followed by confidence-prioritized mask intersection-over-minimum suppression. This two-stage procedure removes redundant observations while preserving the geometry of retained masks. On ten held-out railway signal-layout drawings containing 1755 annotated instances, the validation-selected configuration achieves precision 0.8994, recall 0.9470, and F1 0.9226. With identical candidates and fixed box-NMS/containment thresholds of 0.50/0.60, DSC reduces false positives from 288 to 186 and improves F1 from 0.8989 to 0.9226 relative to box-based containment filtering. Three-seed local-recognition ablations, a joint threshold analysis, and quantitative error and resource measurements characterize the evaluated pipeline. Optical character recognition and railway-specific rules are assessed separately as application refinement. These results support containment-aware consolidation for producing consistent instance inventories from overlapping tiles in the studied engineering drawings. FAU - Kuang, Wenzhen AU - Kuang W AD - Institute of Automatic Control, Lanzhou Jiaotong University, Lanzhou 730070, China. AD - Key Laboratory of Optoelectronic Technology and Intelligent Control Ministry of Education, Lanzhou Jiaotong University, Lanzhou 730070, China. FAU - Tang, Xixuan AU - Tang X AD - Institute of Automatic Control, Lanzhou Jiaotong University, Lanzhou 730070, China. AD - Key Laboratory of Optoelectronic Technology and Intelligent Control Ministry of Education, Lanzhou Jiaotong University, Lanzhou 730070, China. FAU - Fu, Xiao AU - Fu X AD - National Key Laboratory of Human-Machine Hybrid Augmented Intelligence, National Engineering Research Center for Visual Information and Applications, Xi'an 710049, China. AD - School of Computer Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China. FAU - Xi, Wei AU - Xi W AD - National Key Laboratory of Human-Machine Hybrid Augmented Intelligence, National Engineering Research Center for Visual Information and Applications, Xi'an 710049, China. AD - School of Computer Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China. LA - eng PT - Journal Article DEP - 20261010 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13709312 OTO - NOTNLM OT - duplicate suppression OT - engineering drawings OT - high-resolution image analysis OT - instance segmentation OT - railway signal layouts OT - tiled inference COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:47 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - s26206413 [pii] AID - sensors-26-06413 [pii] AID - 10.3390/s26206413 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 10;26(20):6413. doi: 10.3390/s26206413. PMID- 42944274 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 10 TI - Eye-Tracking Analysis of Visual Attention and Preference in Human-Created, AI-Generated, and AI-Refined Human Advertisements. LID - 10.3390/s26206411 [doi] LID - 6411 AB - Generative artificial intelligence (GenAI) is increasingly used in advertising, yet its effects on visual attention and preference remain insufficiently characterized. This study compared human-created (H), AI-generated (AI), and AI-refined human (HAI) advertisements, the latter obtained by submitting human-created advertisements to a generative model with a standardized refinement prompt, using eye tracking and a forced-choice task. Thirty-nine young adults (18-26 years) with high generative-AI experience completed 858 trials involving 22 advertisement pairs while gaze was recorded at 60 Hz. Fixation duration, fixation count, time to first fixation, saccadic magnitude, and pupil diameter were compared using repeated-measures analyses of participant-level means with Holm correction, and choices were analyzed using binomial models with participant-clustered standard errors. AI-generated advertisements were selected more frequently than human-created advertisements (58.97% of trials; odds ratio = 1.44, p=0.0015), whereas HAI advertisements did not differ from human-created advertisements in choice frequency (49.88%; p=0.978). Relative to human-created advertisements, both AI and HAI stimuli were associated with higher fixation counts and smaller saccadic magnitudes, with these effects surviving Holm correction. Time to first fixation was statistically equivalent across conditions within the smallest effect detectable by the design, pupil diameter did not differ, and differences in fixation duration did not survive correction. The higher fixation counts reflected a larger share of fixation time on AI and HAI advertisements rather than a higher fixation rate. Although the gaze pattern was similar for AI and HAI stimuli while only AI stimuli were preferred, the difference between the two choice effects was not statistically reliable. The observed gaze differences cannot be uniquely attributed to creative origin because AI and HAI stimuli also exhibited higher edge density than human-created stimuli. These findings support the use of eye tracking as a sensor-based method for characterizing visual responses to AI-assisted advertising while highlighting the need for tighter stimulus control and more diverse samples in future studies. FAU - Goncalves, Andre AU - Goncalves A AD - Instituto Superior de Engenharia do Porto (ISEP), Rua Dr. Antonio Bernardino de Almeida 431, 4249-015 Porto, Portugal. FAU - Afonso, Margarida AU - Afonso M AD - Research Group on Intelligent Engineering and Computing for Advanced Innovation and Development (GECAD), Instituto Superior de Engenharia do Porto (ISEP), Rua Dr. Antonio Bernardino de Almeida 431, 4249-015 Porto, Portugal. FAU - Martins, Pedro AU - Martins P AUID- ORCID: 0000-0002-2118-1440 AD - Coimbra Business School (ISCAC), Polytechnic University of Coimbra, Rua da Misericordia, Lagar dos Corticos, S. Martinho do Bispo, 3045-093 Coimbra, Portugal. FAU - Teixeira, Ana AU - Teixeira A AUID- ORCID: 0000-0002-8120-0148 AD - Research Group on Intelligent Engineering and Computing for Advanced Innovation and Development (GECAD), Instituto Superior de Engenharia do Porto (ISEP), Rua Dr. Antonio Bernardino de Almeida 431, 4249-015 Porto, Portugal. AD - Center for Research and Innovation in Education (InED), School of Education, Polytechnic of Porto, Rua Dr. Roberto Frias 602, 4200-465 Porto, Portugal. LA - eng PT - Journal Article DEP - 20261010 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM MH - Humans MH - *Attention/physiology MH - Adult MH - *Eye-Tracking Technology MH - Female MH - *Advertising/methods MH - Young Adult MH - Generative Artificial Intelligence MH - Adolescent MH - Male MH - Fixation, Ocular/physiology MH - Eye Movements/physiology MH - *Artificial Intelligence MH - Choice Behavior/physiology MH - Pupil/physiology PMC - PMC13709040 OTO - NOTNLM OT - AI-refined advertising OT - advertising OT - consumer choice OT - eye tracking OT - generative artificial intelligence OT - visual attention OT - visual perception COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/10 CRDT- 2026/10/27 10:47 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - s26206411 [pii] AID - sensors-26-06411 [pii] AID - 10.3390/s26206411 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 10;26(20):6411. doi: 10.3390/s26206411. PMID- 42944273 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 10 TI - Reliability of Myotonometric Measurements and Their Associations with Motor Performance in Parkinson's Disease. LID - 10.3390/s26206410 [doi] LID - 6410 AB - Myotonometry provides a non-invasive assessment of resting muscle mechanical properties, but its reliability and functional relevance in Parkinson's disease (PD) remain insufficiently established. This study examined the within-session intra-rater reliability of MyotonPRO measurements and explored their associations with clinical motor severity, gait, functional mobility, and manual dexterity in 24 people with PD assessed in the ON-medication state. Frequency, stiffness, logarithmic decrement, relaxation time, and creep were measured bilaterally in the biceps brachii, triceps brachii, tibialis anterior, and medial gastrocnemius. Reliability between two sets of five impulses was assessed using ICC(3,2), and associations were examined using Spearman correlations with Benjamini-Hochberg false discovery rate correction. Under the standardized same-session, same-rater protocol, all parameters showed good-to-excellent reliability point estimates (ICC = 0.823-0.988), although the precision of some estimates was limited by relatively wide confidence intervals. No significant associations were found with MDS-UPDRS III scores, Hoehn and Yahr stage, or Timed Up and Go performance. Several moderate nominal correlations were observed between lower-limb myotonometric parameters and preferred gait performance, and between triceps brachii parameters and Grooved Pegboard completion time (|r(s)| = 0.410-0.488); however, none remained significant after false discovery rate correction. Thus, the reliability findings apply to the present standardized protocol, whereas the associations with motor performance remain exploratory and hypothesis-generating. FAU - Latorre-Tomillo, Lucia AU - Latorre-Tomillo L AD - Center for Research in Sport Sciences, Rey Juan Carlos University, Camino del Molino 5, 28942 Fuenlabrada, Madrid, Spain. FAU - Villamil-Cabello, Eduardo AU - Villamil-Cabello E AUID- ORCID: 0000-0002-7231-3183 AD - Center for Research in Sport Sciences, Rey Juan Carlos University, Camino del Molino 5, 28942 Fuenlabrada, Madrid, Spain. FAU - Fogelson, Noa AU - Fogelson N AD - Center for Research in Sport Sciences, Rey Juan Carlos University, Camino del Molino 5, 28942 Fuenlabrada, Madrid, Spain. FAU - Fernandez-Del-Olmo, Miguel Angel AU - Fernandez-Del-Olmo MA AUID- ORCID: 0000-0001-5325-503X AD - Center for Research in Sport Sciences, Rey Juan Carlos University, Camino del Molino 5, 28942 Fuenlabrada, Madrid, Spain. LA - eng GR - PID2022-139930NB-I00/Ministerio de Ciencia, Innovacion y Universidades/ GR - PID2023-150168NB-I00/Ministerio de Ciencia, Innovacion y Universidades/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM MH - Humans MH - *Parkinson Disease/physiopathology MH - Female MH - Male MH - Gait/physiology MH - Reproducibility of Results MH - Aged MH - *Muscle, Skeletal/physiopathology/physiology MH - Middle Aged PMC - PMC13709277 OTO - NOTNLM OT - Parkinson's disease OT - gait OT - myotonometry OT - reliability COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/10 CRDT- 2026/10/27 10:47 PHST- 2026/08/03 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - s26206410 [pii] AID - sensors-26-06410 [pii] AID - 10.3390/s26206410 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 10;26(20):6410. doi: 10.3390/s26206410. PMID- 42944269 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 10 TI - Dust Contamination and Environmental Cofactor Effects on Flexible Sensors and Antennas: A Review. LID - 10.3390/s26206407 [doi] LID - 6407 AB - Flexible sensors and antennas are increasingly deployed in wearable health monitoring, environmental sensing, and RFID technology, yet their reliability under environmental exposure remains insufficiently addressed. This review synthesizes the literature on the environmental degradation of flexible sensors and antennas, with particulate-induced degradation as the primary stressor and humidity and temperature treated as cofactors; additional mechanisms such as flexural fatigue and UV-induced photodegradation are briefly addressed but beyond the scope. Across the literature, dust-induced degradation rarely acts independently: moisture ingress within the substrate repeatedly appears as a shared vulnerability that could: (a) shift dielectric properties, (b) enable moisture transport about the conductor surface, and (c) promote electrolyte formation through ionic particulate deposition, accelerating electrochemical degradation. Temperature further amplifies these mechanisms by accelerating moisture diffusion and reaction kinetics and by promoting the generation of microcracks through CTE mismatch and cyclic fatigue, which create sites for moisture ingress and particulate deposition. We conclude with a layered mitigation architecture spanning encapsulation, substrate selection, conductor and material engineering, and geometric design. Building on this architecture, we propose a provisional (prototype-like) three-axis design framework that classifies deployment environments by aqueous ionic conductivity of particulate deposits, sustained relative humidity, and operating temperature with cycling amplitude, mapping each of the 27 combinations to a candidate mitigation prescription. We identify key experimental gaps; most notably, the scarcity of dust deposition studies on flexible sensors and antennas and the absence of comparative substrate testing across the three stressors, simultaneously. Addressing these knowledge gaps could enable the development of mechanically robust, environmentally resilient flexible systems for wearable technologies, while also facilitating the exploitation of dust-induced surface degradation as a functional transduction mechanism for sensing applications. A Limitation Section is included to highlight the fact that sufficient particulate-deposition studies on flexible sensors and antennas remain elusive, which provides opportunities for future research. While some mechanistic effects and material characterization are extrapolated from rigid PCB and MEMS studies, this does not constitute direct experimental support for their flexible counterparts. FAU - Medina, Hector AU - Medina H AD - School of Engineering, Liberty University, Lynchburg, VA 24515, USA. FAU - Kries, Eli AU - Kries E AD - School of Engineering, Liberty University, Lynchburg, VA 24515, USA. LA - eng PT - Journal Article PT - Review DEP - 20261010 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13709140 OTO - NOTNLM OT - electrochemical degradation OT - encapsulation OT - environmental degradation OT - flexible antennas OT - flexible sensors OT - humidity OT - mitigation strategies OT - moisture ingress OT - particulate contamination OT - temperature variation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:47 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - s26206407 [pii] AID - sensors-26-06407 [pii] AID - 10.3390/s26206407 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 10;26(20):6407. doi: 10.3390/s26206407. PMID- 42944268 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 10 TI - An Optimized Audio-Visual Deepfake Detection Framework Using Mamba Vision Transformers with Linear Complexity. LID - 10.3390/s26206406 [doi] LID - 6406 AB - The rise of advanced deepfake technologies presents new challenges to media authenticity, making it harder to spot manipulated videos through traditional approaches. Current approaches have a quadratic time complexity O(n2), poor cross-dataset generalization with 20 to 35% accuracy degradation, and inadequate audio-visual fusion mechanisms. This paper presents Mamba-AVGuard, a novel audio-visual deepfake detection framework leveraging Mamba Vision Transformers with selective state-space modeling to achieve linear O(n) complexity within the Mamba encoder streams. The novel framework combines a 12-layer visual Mamba encoder for spatial and temporal facial inconsistency analysis with an 8-layer lightweight audio Mamba encoder for prosodic inconsistency analysis, fused via a cross-modal attention block with +/-33 ms temporal synchronization. Experiments conducted on two large-scale audio-visual deepfake benchmarks, FakeAVCeleb and AV-Deepfake1M, demonstrate that Mamba-AVGuard outperforms compared methods with 99.92% in-dataset accuracy and 98.47% cross-dataset accuracy on these two benchmarks. The system achieves a processing speed of less than 1.8 s for 30-s videos, with 56% less memory than the best competing transformer-based method, facilitating a throughput of more than 1200 videos per hour for social media verification and forensic workstations. FAU - Arain, Aisha AU - Arain A AD - Department of Software Engineering, Mehran University of Engineering and Technology, Jamshoro 76062, Pakistan. FAU - Naz, Bushra AU - Naz B AD - Department of Software Engineering, Mehran University of Engineering and Technology, Jamshoro 76062, Pakistan. FAU - Bhatti, Sania AU - Bhatti S AUID- ORCID: 0000-0002-0887-8083 AD - Department of Software Engineering, Mehran University of Engineering and Technology, Jamshoro 76062, Pakistan. FAU - Alhwikem, Faisal AU - Alhwikem F AUID- ORCID: 0009-0001-7632-7988 AD - Department of Computer Science, College of Computer, Qassim University, Buraydah 51452, Saudi Arabia. FAU - Jaskani, Fawwad Hassan AU - Jaskani FH AUID- ORCID: 0000-0002-0922-6829 AD - Department of Computer Systems Engineering, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan. LA - eng GR - QU-APC-2026/Qassim University/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13709306 OTO - NOTNLM OT - Mamba Vision Transformer OT - audio-visual fusion OT - deepfake detection OT - media forensics OT - multimodal learning OT - real-time detection OT - state-space models COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:47 PHST- 2026/07/13 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - s26206406 [pii] AID - sensors-26-06406 [pii] AID - 10.3390/s26206406 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 10;26(20):6406. doi: 10.3390/s26206406. PMID- 42944265 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 10 TI - Initial Orbit Determination of Low-Earth-Orbiting Objects Using Multi-Site Space-Based Optical Observations. LID - 10.3390/s26206400 [doi] LID - 6400 AB - Space Situational Awareness (SSA) is a growing field of study which provides knowledge of Resident Space Objects (RSOs) to help maintain a safe and sustainable space environment around Earth. A primary component of SSA is obtaining Initial Orbit Determinations (IODs) of these objects via optical observations. The present way of obtaining optical observations is by using a single observation site. However, using only one observation site does not yield accurate range or IOD results. This research tests space-based single-site and multi-site observations of RSOs in Low Earth Orbit (LEO) to provide the necessary information to calculate IODs. Gooding's algorithm is used to calculate IODs from the simulated data, since it is the most robust IOD algorithm discussed in the literature. The results from this research demonstrate that selected multi-site observations produce more accurate range and IOD results than single-site observations and, thus, provide advancement opportunities for various SSA efforts. FAU - Veltri, Marianna AU - Veltri M AUID- ORCID: 0009-0009-4362-8782 AD - Department of Earth and Space Science, York University, Toronto, ON M3J 1P3, Canada. FAU - Suthakar, Vithurshan AU - Suthakar V AUID- ORCID: 0009-0009-7444-2825 AD - Department of Earth and Space Science, York University, Toronto, ON M3J 1P3, Canada. FAU - Lee, Regina S K AU - Lee RSK AD - Department of Earth and Space Science, York University, Toronto, ON M3J 1P3, Canada. LA - eng GR - RGPIN-2025-06284/Natural Sciences and Engineering Research Council of Canada/ GR - DGDND-2025-06284/the Department of National Defence (Canada) and NSERC through the DND/NSERC Discovery Grant Supplement/ GR - 23FAYORA06/Canadian Space Agency/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13708936 OTO - NOTNLM OT - Initial Orbit Determination OT - Space Situational Awareness OT - low Earth orbit OT - multi-site OT - optical observations OT - resident space objects OT - single-site OT - space-based OT - star-trackers COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:47 PHST- 2026/07/22 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - s26206400 [pii] AID - sensors-26-06400 [pii] AID - 10.3390/s26206400 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 10;26(20):6400. doi: 10.3390/s26206400. PMID- 42944261 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 10 TI - Local Voronoi Computation and Sweep Line Intersection for Efficient Voronoi-Based Grouping Test Localization in Wireless Sensor Networks. LID - 10.3390/s26206397 [doi] LID - 6397 AB - Node localization is a fundamental service in wireless sensor networks (WSNs), and range-free schemes such as the Voronoi diagram-based grouping test localization (VTL) achieve good accuracy without distance measurements. However, VTL repeatedly constructs Voronoi diagrams over all anchor nodes of each group and resolves the intersection of positive regions by an exhaustive grid scan, which limits its scalability. This paper proposes and evaluates two optimizations: (1) a local Voronoi computation that restricts the computation to the k anchors nearest to the strongest-signal anchor (including that anchor itself), and (2) an exact sweep line algorithm that replaces the grid scan in the intersection phase. Both were evaluated in a purpose-built, reproducible simulator through 5184 primary and 464 supplementary simulations spanning 60-1000 nodes, two deployment areas (200 m and 400 m squares) and k = 6-20, with paired Wilcoxon signed-rank tests. The local computation reduced the Voronoi-phase time by 69-83%, with the accuracy penalty falling below 2% for k >/= 10 on average across network sizes, while the percentage of localized nodes remained unchanged. The sweep line algorithm reduced the intersection-phase time by 7.7-18.1% while producing identical localization results. Combined, the two optimizations reduced the total execution time by 37-61% (up to 4.7-fold at 1000 nodes). The conclusions are robust across anchor densities of 20-70%, ten independent seeds, and log-normal shadowing noise in the anchor selection. We recommend k = 10-12 as the default operating range, whereas k = 6 degrades both accuracy and overall speed. FAU - Dambic, Goran AU - Dambic G AD - Algebra Bernays University, Gradiscanska 24, 10000 Zagreb, Croatia. FAU - Percinic, Patrik AU - Percinic P AUID- ORCID: 0009-0006-1287-361X AD - Algebra Bernays University, Gradiscanska 24, 10000 Zagreb, Croatia. FAU - Ogrizek Biskupic, Ivana AU - Ogrizek Biskupic I AUID- ORCID: 0009-0002-0947-9655 AD - Algebra Bernays University, Gradiscanska 24, 10000 Zagreb, Croatia. FAU - Radovan, Aleksander AU - Radovan A AUID- ORCID: 0000-0001-6905-091X AD - Algebra Bernays University, Gradiscanska 24, 10000 Zagreb, Croatia. LA - eng PT - Journal Article DEP - 20261010 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13708900 OTO - NOTNLM OT - Voronoi diagram OT - computational efficiency OT - node localization OT - range-free localization OT - simulation OT - sweep line algorithm OT - wireless sensor networks COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:47 PHST- 2026/08/26 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - s26206397 [pii] AID - sensors-26-06397 [pii] AID - 10.3390/s26206397 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 10;26(20):6397. doi: 10.3390/s26206397. PMID- 42944254 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 9 TI - Longitudinally and Vertically Balanced Sparse Indoor Temperature-Humidity Sensing Preserves Threshold-Defined Risk Inference in a Chinese Solar Greenhouse. LID - 10.3390/s26206391 [doi] LID - 6391 AB - Sensor failures reduce the spatial information available for greenhouse microclimate monitoring, but their practical consequences depend on whether agronomic risk decisions remain reliable. We evaluated the progressive loss of paired indoor temperature-relative humidity (T/RH) sensors in a Chinese solar greenhouse using whole-sensor holdout. Longitudinally and vertically balanced subsets and transparent estimators were frozen before a sealed final test of 28 dates, four calendar blocks and six stage-aware risks. Relative to a 20-input reference, a longitudinally and vertically balanced six-input network increased the temperature mean absolute error by 0.014 degrees C and the relative-humidity error by 0.027 percentage points; macro-recall changed by -0.001 and support-aware macro-F1 by -0.004, while median false-negative exposure remained unchanged. The results remained within the predefined limits across 26 agricultural-threshold variants. In an exploratory validation-only comparison, spatially concentrated six-input layouts had greater temperature error (+0.080 degrees C, 95% block-bootstrap interval +0.058 to +0.104 degrees C), relative-humidity error (+0.099 percentage points), and macro-F1 loss (-0.004) than longitudinally and vertically balanced layouts. Macro-F1 did not differ consistently between the longitudinally and vertically balanced layouts and the unconstrained random layouts. Longitudinal and vertical coverage therefore functions as a robustness constraint against concentrated input loss in this system, rather than evidence of universal six-sensor sufficiency or cross-greenhouse generalisation. FAU - Shi, Junjie AU - Shi J AUID- ORCID: 0009-0000-9754-4781 AD - School of Energy and Power Engineering, Lanzhou University of Technology, Lanzhou 730050, China. AD - Key Laboratory of Multi-Supply System with Solar Energy and Biomass, Lanzhou University of Technology, Lanzhou 730050, China. AD - China Northwest Collaborative Innovation Center of Low-Carbon Urbanization Technologies, Lanzhou 730050, China. AD - School of Energy and Power Engineering, Lanzhou Petrochemical University of Vocational Technology, Lanzhou 730060, China. FAU - Li, Jinping AU - Li J AD - Key Laboratory of Multi-Supply System with Solar Energy and Biomass, Lanzhou University of Technology, Lanzhou 730050, China. AD - China Northwest Collaborative Innovation Center of Low-Carbon Urbanization Technologies, Lanzhou 730050, China. AD - School of Green Energy and Storage, Lanzhou University of Technology, Lanzhou 730050, China. FAU - Wei, Yanhong AU - Wei Y AD - School of Mechanical Engineering, Lanzhou Petrochemical University of Vocational Technology, Lanzhou 730060, China. FAU - Zhang, Chong AU - Zhang C AD - Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China. FAU - Ma, Ya AU - Ma Y AD - School of Petroleum and Chemical Engineering, Lanzhou Petrochemical University of Vocational Technology, Lanzhou 730060, China. FAU - Yang, Wenjie AU - Yang W AD - School of Mechanical Engineering, Lanzhou Petrochemical University of Vocational Technology, Lanzhou 730060, China. FAU - Fan, Zhiwei AU - Fan Z AD - Department of Infrastructure Development and Support, Tian Hua Institute of Chemical Machinery & Automation Co., Ltd., Lanzhou 730060, China. LA - eng GR - 24ZDNA004/Gansu Major Science and Technology Projects/ GR - [2024]12/Gansu Top Leading Talents Project/ GR - 25JRRA059/Gansu Fundamental Research Innovation Group Project/ GR - 2026CYZC-023/Gansu Provincial Higher Education Research Project/ GR - 2022CYZC-73/Industrial Support Plan Project for Colleges and Universities in Gansu Province/ GR - 2025B-313/Education Science and Technology Innovation Project of Gansu Province/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13708978 OTO - NOTNLM OT - agronomic risk OT - greenhouse monitoring OT - longitudinal and vertical coverage OT - selective inference OT - sensor networks OT - smart agriculture OT - sparse sensing OT - temperature and relative humidity OT - whole-sensor holdout COIS- Zhiwei Fan is employed by Tian Hua Institute of Chemical Machinery & Automation Co., Ltd. The employer provided no funding, equipment, data, analytical support, APC support, or intellectual-property or commercial interests related to this study. The employer had no role in study design; data collection, analysis, or interpretation; manuscript preparation; or the decision to publish. The authors declare no other competing interests. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:47 PHST- 2026/07/31 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - s26206391 [pii] AID - sensors-26-06391 [pii] AID - 10.3390/s26206391 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 9;26(20):6391. doi: 10.3390/s26206391. PMID- 42944253 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 9 TI - Enhancing the Reliability of NR-V2X Sidelink Broadcast Through Relaying and Dynamic Retransmission Control. LID - 10.3390/s26206392 [doi] LID - 6392 AB - NR-V2X sidelink broadcast is an important communication mode for delivering safety-related information to surrounding vehicles with low latency. However, in non-line-of-sight (NLOS) environments such as intersections, direct links are blocked, and the blind retransmission mechanism defined for NR-V2X provides limited reliability improvement. This paper extends our previously reported relay-assisted retransmission scheme, RRT + ACK, which combines relay selection using link-quality indicators aggregated per direction (AvgLQIs/Dir) with ACK-assisted transfer of retransmission responsibility to the selected relay. The extension is a dynamic retransmission control (RT-Ctrl) method based on the Channel Busy Ratio (CBR), intended to limit retransmission-induced congestion. Simulations using Scenargie in NLOS intersection environments, under the assumption of ideal error-free ACK delivery, show that the basic RRT + ACK scheme improves the evaluation packet delivery ratio within 200 m (EPDR200m) by approximately 8.6% compared with conventional blind retransmission. Under high vehicle density, the RT-Ctrl method with the best parameter setting improves EPDR200m from 0.783 to 0.867 and reduces the average CBR from 0.594 to 0.329 compared with RRT + ACK using a fixed retransmission count. FAU - Takahashi, Neo AU - Takahashi N AD - Graduate School of Informatics and Engineering, The University of Electro-Communications, Chofu 182-8585, Tokyo, Japan. FAU - Aoki, Shiro AU - Aoki S AD - Graduate School of Informatics and Engineering, The University of Electro-Communications, Chofu 182-8585, Tokyo, Japan. FAU - Tang, Suhua AU - Tang S AUID- ORCID: 0000-0002-5784-8411 AD - Graduate School of Informatics and Engineering, The University of Electro-Communications, Chofu 182-8585, Tokyo, Japan. LA - eng PT - Journal Article DEP - 20261009 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13709126 OTO - NOTNLM OT - NR-V2X OT - blind retransmission OT - broadcast OT - channel busy ratio OT - congestion control OT - relay OT - sidelink COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:47 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - s26206392 [pii] AID - sensors-26-06392 [pii] AID - 10.3390/s26206392 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 9;26(20):6392. doi: 10.3390/s26206392. PMID- 42944246 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 9 TI - MIDAS: Multi-Sensorial Immersive Dynamic Autonomous System for Stroke Hand Rehabilitation-Prototype Design and Exploratory User Evaluation. LID - 10.3390/s26206384 [doi] LID - 6384 AB - Many stroke survivors experience impaired function in their paretic hands. Maintaining motivation to continue hand rehabilitation exercises can be challenging. This paper presents MIDAS (Multi-Sensorial Immersive Dynamic Autonomous System), a proof-of-concept immersive system developed to explore feasibility, user experience, and self-reported responses during hand rehabilitation after stroke. MIDAS is lightweight and portable. It consists of a hand exoskeleton subsystem, a virtual reality (VR) subsystem, and an olfactory subsystem. Together, these components engage four senses during rehabilitation. A pilot study involving five people affected by stroke was conducted across three sessions. The subsystems of MIDAS were added progressively in each session. The game environment, sound effects, and scent were selected to create a multisensory experience. User experience scores were higher in Session 3 than in Session 1 for four participants and unchanged for one. Stroke Rehabilitation Motivation Scale (SRMS) scores decreased across sessions for two participants, increased between Sessions 1 and 3 for two, and remained unchanged for one. Responses to several individual questionnaire items were favorable, but the study did not establish an overall increase in rehabilitation motivation. FAU - Sinha, Anoop Kumar AU - Sinha AK AD - School of Mechanical and Aerospace Engineering, Nanyang Technological University, 65 Nanyang Dr, Singapore 637460, Singapore. AD - Currently with the School of Science and Technology, Singapore University of Social Sciences, 463 Clementi Road, Singapore 599494, Singapore. AD - Institute for Media Innovation (IMI), Nanyang Technological University (NTU), Singapore 637553, Singapore. FAU - Kow, Fok-Chi-Seng Fok AU - Kow FF AD - School of Art, Design and Media, Nanyang Technological University, 81 Nanyang Dr, Singapore 637458, Singapore. FAU - Ming, Zhang Jin AU - Ming ZJ AD - School of Art, Design and Media, Nanyang Technological University, 81 Nanyang Dr, Singapore 637458, Singapore. FAU - Songyu, Bao AU - Songyu B AD - School of Art, Design and Media, Nanyang Technological University, 81 Nanyang Dr, Singapore 637458, Singapore. FAU - Kang, Jake Tan Jun AU - Kang JTJ AD - School of Art, Design and Media, Nanyang Technological University, 81 Nanyang Dr, Singapore 637458, Singapore. FAU - Jeffrey, Hong Yan Jack AU - Jeffrey HYJ AD - School of Art, Design and Media, Nanyang Technological University, 81 Nanyang Dr, Singapore 637458, Singapore. FAU - Mihaleva, Galina AU - Mihaleva G AD - School of Art, Design and Media, Nanyang Technological University, 81 Nanyang Dr, Singapore 637458, Singapore. FAU - Thalmann, Nadia Magnenat AU - Thalmann NM AD - Institute for Media Innovation (IMI), Nanyang Technological University (NTU), Singapore 637553, Singapore. FAU - Cai, Yiyu AU - Cai Y AUID- ORCID: 0000-0002-8406-9536 AD - School of Mechanical and Aerospace Engineering, Nanyang Technological University, 65 Nanyang Dr, Singapore 637460, Singapore. LA - eng GR - Accelerating Creativity and Excellence (ACE) grant/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM MH - Humans MH - *Stroke Rehabilitation/methods MH - *Hand/physiopathology MH - Virtual Reality MH - Female MH - Male MH - Middle Aged MH - *Stroke/physiopathology MH - Pilot Projects MH - Exoskeleton Device MH - Aged MH - Motivation PMC - PMC13708756 OTO - NOTNLM OT - 3D printing OT - hand exoskeleton OT - immersive game OT - olfactory device OT - pilot study OT - rehabilitation OT - self-rehabilitation motivation OT - user experience OT - virtual reality COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/09 CRDT- 2026/10/27 10:47 PHST- 2026/07/31 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - s26206384 [pii] AID - sensors-26-06384 [pii] AID - 10.3390/s26206384 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 9;26(20):6384. doi: 10.3390/s26206384. PMID- 42944239 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 9 TI - Multi-Feature Classification of Displacement Alarm Causes Using IoT-Based Slope Monitoring Sensor Data. LID - 10.3390/s26206378 [doi] LID - 6378 AB - Internet of Things (IoT)-based slope monitoring systems support real-time stability surveillance, but frequent false alarms cause alarm fatigue and delay responses to genuine hazards. We developed a clustering-based framework that classifies alarm causes into three categories-Malfunction, Actual Displacement, and Others-from inclinometer and extensometer data. From 55 alarm events recorded at 43 monitoring stations in 2025, 85 domain-informed features (statistical, waveform, spatial-correlation, trend, sudden-synchronization, and malfunction-pattern) were extracted and compared across five feature-extraction methods, six selection levels, and three clustering algorithms. Because the feature ranking uses expert-assigned labels, the framework is characterized as label-informed feature ranking followed by unsupervised clustering, and it was evaluated with 100 random seeds, nested leave-one-site-out validation, bootstrap confidence intervals, and permutation tests. A compact set of five features-three cross-correlation-based synchronization features and two spatial-correlation features-was stable across seeds and achieved a leave-one-site-out accuracy of 0.80 (95% CI 0.69-0.89) with the full 8 h window. Using only data recorded up to the alarm time, accuracy rose to 0.91 (0.84-0.98), 12 of the 13 Actual Displacement events were recovered (recall 0.92), and no non-displacement event was assigned to the displacement cluster. Synchronization features separated genuine displacement from the other causes, whereas spatial-correlation features distinguished external disturbance from sensor malfunction. The interpretable framework is proposed as a decision-support filter that complements conventional threshold-based alarms. FAU - Lee, Sang-Yun AU - Lee SY AD - Integrated Road Management Research Center, Korea Institute of Civil Engineering and Building Technology (KICT), Goyang-si 10223, Republic of Korea. FAU - Park, Byung-Suk AU - Park BS AD - Integrated Road Management Research Center, Korea Institute of Civil Engineering and Building Technology (KICT), Goyang-si 10223, Republic of Korea. FAU - Kim, Woo-Seok AU - Kim WS AUID- ORCID: 0000-0002-9945-8593 AD - Department of Geotechnical Engineering Research, Korea Institute of Civil Engineering and Building Technology (KICT), Goyang-si 10223, Republic of Korea. FAU - Hwang, Sung-Pil AU - Hwang SP AUID- ORCID: 0000-0003-4390-9348 AD - Department of Geotechnical Engineering Research, Korea Institute of Civil Engineering and Building Technology (KICT), Goyang-si 10223, Republic of Korea. LA - eng GR - RS-2025-02263904/Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET) through the Intelligent Agricultural Infra Management for Climate Change Development Program, funded by the Ministry of Agriculture, Food and Rural Affairs/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13709250 OTO - NOTNLM OT - IoT sensor OT - clustering OT - displacement cause classification OT - feature extraction OT - multi-sensor analysis OT - slope monitoring COIS- The authors declare no relevant financial or non-financial interests. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:47 PHST- 2026/07/30 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - s26206378 [pii] AID - sensors-26-06378 [pii] AID - 10.3390/s26206378 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 9;26(20):6378. doi: 10.3390/s26206378. PMID- 42944236 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 9 TI - Line-Scan Defect Detection for Printed Laminated Films Using Multi-Template Matching and Residual-Driven Column-Band Compensation. LID - 10.3390/s26206372 [doi] LID - 6372 AB - Machine vision enables efficient, non-contact industrial quality inspection. Online inspection of printed laminated films is challenging because complex graphics and transport-induced stretching cause appearance variations and spatially nonuniform mismatches. Global registration cannot fully correct these mismatches, whereas unrestricted dense deformation may absorb small defects. We propose an interpretable system based on a qualified multi-template library and residual-driven column-band compensation. Low-resolution retrieval selects geometrically compatible references; intermediate-resolution ORB-RANSAC registration and original-resolution warping then establish global correspondence. Compensation is activated only in residual-selected bands and constrained to the web-motion direction. Template-tolerant multi-scale Frangi comparison and component-level multi-template voting generate the final defect mask. The independent test set comprised 1500 production-line images: 200 defect-bearing images containing 823 annotated defect instances and 1300 defect-free images. Under the defect-instance-level evaluation, the system produced 765 true positives, 58 false negatives, and 31 false-positive predicted components, corresponding to 92.95% recall and 96.11% precision. The mean processing time was 357.06 ms/image. The results indicate that the method can balance residual-mismatch suppression and defect preservation under the evaluated production conditions. FAU - Zhang, Pengjian AU - Zhang P AD - College of Mechanical and Electrical Engineering, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Ren, Wen AU - Ren W AUID- ORCID: 0000-0002-3605-7274 AD - School of Mechanical and Electrical Engineering, Sanming University, Sanming 365004, China. LA - eng GR - 2025I0031/Foreign Cooperation Projects of Fujian Province/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13708870 OTO - NOTNLM OT - defect detection OT - deformation compensation OT - line-scan imaging OT - multi-template matching OT - printed laminated films COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:47 PHST- 2026/09/04 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - s26206372 [pii] AID - sensors-26-06372 [pii] AID - 10.3390/s26206372 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 9;26(20):6372. doi: 10.3390/s26206372. PMID- 42944231 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 9 TI - Reconfigurable ADAMS Modeling of Localized Bearing Defects: Validation and the Role of Modeling Choices in Dynamic Response. LID - 10.3390/s26206370 [doi] LID - 6370 AB - Localized defects in rolling-element bearings produce vibration responses governed by contact events, load transmission, defect geometry, and system dynamics. To examine these mechanisms in a controlled and reconfigurable setting, this paper presents an MSC ADAMS framework for localized bearing faults, demonstrated on an SKF 6208 deep groove ball bearing with outer-ring spalls. The framework is evaluated against experimental test-rig measurements and a legacy 3D MATLAB bearing model, with healthy bearing contact forces checked against analytical load-distribution theory. The ADAMS model reproduces the spectral structure (including BPFO harmonics and selected modulation features), defect-interaction timing, and trends across defect sizes, though not absolute vibration amplitudes, while providing access to internal contact forces during defect interaction. This combination is used to examine two modeling issues that affect the mechanical interpretation of simulated bearing fault response. First, the outer-ring support definition controls the effective load path: adding support stiffness can transfer part of the applied load directly to the ground, reducing the load carried through rolling contacts. Second, the defect exit response depends on local contact formulation and geometric discretization. The legacy MATLAB model, based on a single-point Hertzian contact formulation, produces a sharper exit rebound, whereas the geometry-based ADAMS contact produces a more gradual return to the load zone. The study therefore contributes not only a validated simulation framework, but also mechanistic insight into how support and contact modeling choices shape the vibration and contact force responses used in bearing fault diagnostics, prognostics, and physical interpretation. FAU - Schwarcz, Ori AU - Schwarcz O AUID- ORCID: 0009-0008-9138-6039 AD - Prognostic Health Monitoring Laboratory, Department of Mechanical Engineering, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 8410501, Israel. FAU - Bublil, Tal AU - Bublil T AUID- ORCID: 0009-0003-0277-6347 AD - Prognostic Health Monitoring Laboratory, Department of Mechanical Engineering, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 8410501, Israel. FAU - Bortman, Jacob AU - Bortman J AUID- ORCID: 0000-0002-8910-6738 AD - Prognostic Health Monitoring Laboratory, Department of Mechanical Engineering, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 8410501, Israel. FAU - Shoham, Shahar AU - Shoham S AD - Prognostic Health Monitoring Laboratory, Department of Mechanical Engineering, Ben-Gurion University of the Negev, P.O. Box 653, Beer-Sheva 8410501, Israel. LA - eng GR - Pearlstone Center for Aeronautical Engineering Studies, Ben-Gurion University of the Negev/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13709071 OTO - NOTNLM OT - MSC ADAMS OT - contact mechanics OT - localized bearing defects OT - multibody dynamics OT - rolling-element bearings COIS- The authors declare no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:47 PHST- 2026/08/01 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - s26206370 [pii] AID - sensors-26-06370 [pii] AID - 10.3390/s26206370 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 9;26(20):6370. doi: 10.3390/s26206370. PMID- 42944230 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 9 TI - OpenTriIDS: Confidence-Guided Open-Set Intrusion Triage for Unknown Attack Detection in IoT Devices. LID - 10.3390/s26206369 [doi] LID - 6369 AB - Open-set IoT intrusion detection must distinguish unfamiliar attacks from uncertain known traffic. OpenTriIDS implements a three-action policy: a random forest accepts high-confidence predictions, routes uncertain samples to a compact known-class prototype memory, and rejects routed samples beyond a standardized Euclidean distance boundary. At the representative S1 operating points on Edge-IIoTset and CIC-IoT-2023, the operational macro-F1 reaches 68.44% and 76.81%, with routing rates of 4.53% and 4.13%, respectively. The representative S1 analysis shows that the confidence gate defines the selected subset, while the three-prototype support rule improves macro-F1 and changes the balance between known-class retention and unknown rejection; the magnitude and direction depend on the dataset and operating point. The prototype-distance module records the matched label and distance for each routed sample. A complementary controlled protocol evaluates three seeds across multiple held-out-family settings, while a seven-method S1 comparison evaluates MSP, OpenMax, ORI open recognition, EFC, post hoc Energy OOD, single-layer Mahalanobis scoring, and OpenTriIDS at their documented operating points. OpenTriIDS achieves the highest macro-F1 among the evaluated methods in both datasets under this comparison. Together, the analyses characterize a selective open-set policy whose matched-gate macro-F1 advantage is accompanied by dataset- and holdout-dependent unknown-recall trade-offs, while distinguishing predictive performance from routing and computational cost. FAU - Wu, Xuan AU - Wu X AUID- ORCID: 0009-0006-7435-8584 AD - College of Air and Missile Defense, Air Force Engineering University, Xi'an 710051, China. FAU - Wang, Peng AU - Wang P AUID- ORCID: 0000-0001-5552-0618 AD - Academy of Military Science, Beijing 100091, China. FAU - Song, Yafei AU - Song Y AUID- ORCID: 0000-0003-0962-0671 AD - College of Air and Missile Defense, Air Force Engineering University, Xi'an 710051, China. FAU - Wang, Xiaodan AU - Wang X AUID- ORCID: 0000-0003-2785-9539 AD - College of Air and Missile Defense, Air Force Engineering University, Xi'an 710051, China. LA - eng GR - 61876189/National Natural Science Foundation of China/ GR - 61273275/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13709266 OTO - NOTNLM OT - IoT security OT - intrusion detection OT - open-set recognition OT - prototype rejection OT - selective classification OT - sensor network traffic OT - unknown attack detection COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:47 PHST- 2026/08/30 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - s26206369 [pii] AID - sensors-26-06369 [pii] AID - 10.3390/s26206369 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 9;26(20):6369. doi: 10.3390/s26206369. PMID- 42944227 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 9 TI - Function-Decoupled Environment-Gated Multimodal Fusion for Chlorophyll-a Retrieval in a Cold-Arid Shallow Lake. LID - 10.3390/s26206362 [doi] LID - 6362 AB - Lake Ulansuhai is a eutrophic shallow lake in the cold-arid region of northern China, where shallow water depth, frequent wind disturbance, and heterogeneous optical conditions complicate satellite-based chlorophyll-a (Chl-a) retrieval. To improve retrieval under such complex observation conditions, this study develops a function-decoupled environment-gated fusion framework (FDEGF) that integrates optical, synthetic aperture radar (SAR), and meteorological observations through predefined computational roles. Sentinel-2 optical features form the direct retrieval pathway, Sentinel-1 SAR features provide auxiliary information, and ERA5 meteorological variables condition the participation of the auxiliary pathway at the sampling-event level. A satellite-ground matched dataset containing 287 valid observations from 2017 to 2019 was constructed for Lake Ulansuhai. Across 31 paired random train-test splits, FDEGF showed a generally positive absolute-error advantage relative to simplified fusion structures, reducing median MAE by 0.360-0.467mugL-1. Further analyses showed that model performance depended on the correspondence between event-level meteorological gating and the SAR auxiliary pathway, while the three input pathways exhibited differentiated model dependence. Under unseen-station validation, FDEGF retained positive spatial transferability within the lake. However, leave-one-sampling-event-out validation yielded a pooled R2 of -0.144 and no consistent advantage over the structural controls. These results indicate that explicitly separating direct retrieval, auxiliary information, and meteorological gating provides a structurally explicit strategy for multimodal Chl-a retrieval within the represented sampling-event domain, while transfer to previously unseen sampling events remains limited. FAU - Li, Huimin AU - Li H AUID- ORCID: 0009-0001-1678-161X AD - College of Computer and Information Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China. FAU - Li, Honghui AU - Li H AUID- ORCID: 0009-0003-3436-7150 AD - College of Computer and Information Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China. FAU - Fu, Xueliang AU - Fu X AD - College of Computer and Information Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China. FAU - Shi, Xiaohong AU - Shi X AD - College of Water Conservancy and Civil Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China. LA - eng GR - U25A20528/National Natural Science Foundation of China/ GR - 2025KJTW0026/the Open Competition Mechanism to Select the Best Candidates Project under the Breakthrough Initiative of Inner Mongolia Autonomous Region/ GR - 2025ZD012/the Key Program of the Natural Science Foundation of Inner Mongolia Autonomous Region/ GR - BR231302/the Collaborative Innovation Research Team Project for High-Quality Development of the Agricultural Digital Industry of Inner Mongolia Autonomous Region/ GR - U2443225/National Natural Science Foundation of China/ GR - JC202006/the Basic Discipline Research Startup Fund of Inner Mongolia Agricultural University/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13709113 OTO - NOTNLM OT - Chlorophyll-a retrieval OT - Sentinel-1 SAR OT - Sentinel-2 OT - environment-gated fusion OT - multimodal sensor fusion OT - shallow lakes COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:47 PHST- 2026/08/24 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - s26206362 [pii] AID - sensors-26-06362 [pii] AID - 10.3390/s26206362 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 9;26(20):6362. doi: 10.3390/s26206362. PMID- 42944226 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1424-8220 (Electronic) IS - 1424-8220 (Linking) VI - 26 IP - 20 DP - 2026 Oct 9 TI - Electric-Field-Controlled Rotational Spectroscopy for Highly Selective Gas Sensing. LID - 10.3390/s26206363 [doi] LID - 6363 AB - Air quality control is essential for preventing human exposure to toxic and harmful gases and therefore requires reliable and highly selective gas sensors. Over the past few decades, numerous sensing techniques based on different physical and chemical interactions with sensitive materials have been developed. These include optical, calorimetric, acoustic, and electrical sensing methods such as metal-oxide semiconductor sensors. Although significant progress has been achieved in sensitivity-reaching detection limits at the parts-per-billion level-the precise analysis of complex gas mixtures remains a major challenge due to limited selectivity and cross-sensitivity. In this work, we propose a new gas-sensing mechanism based on the interaction of molecular rotational states with a quasistatically swept DC electric field. For molecules possessing permanent electric dipole moments, the presence of a static electric field produces Stark splitting of rotational levels that is characteristic of each molecular species. The proposed scheme enables a single-frequency detector to analyze gas mixtures with high selectivity, accuracy, and sensitivity. The sensing mechanism relies on species-specific Stark interactions and resonant coupling of molecular dipoles to the applied electric field. Preliminary calculations are presented for the molecules H(2)O and NH(3), illustrating the feasibility of the method. These results suggest that electric-field-controlled rotational spectroscopy provides a promising pathway toward highly selective gas sensing in complex molecular environments. FAU - Brankovic, Zorica AU - Brankovic Z AUID- ORCID: 0000-0001-7231-8235 AD - University of Belgrade - Institute for Multidisciplinary Research, National Institute of the Republic of Serbia, Kneza Viseslava 1, 11030 Belgrade, Serbia. FAU - Brankovic, Goran AU - Brankovic G AUID- ORCID: 0000-0002-4151-1208 AD - University of Belgrade - Institute for Multidisciplinary Research, National Institute of the Republic of Serbia, Kneza Viseslava 1, 11030 Belgrade, Serbia. FAU - Despotovic, Zeljko AU - Despotovic Z AUID- ORCID: 0000-0003-2977-6710 AD - University of Belgrade - Institute Mihailo Pupin, Volgina 15, 11000 Belgrade, Serbia. FAU - Rostovtsev, Yuri AU - Rostovtsev Y AUID- ORCID: 0000-0002-0864-819X AD - Department of Physics and Center for Nonlinear Sciences, University of North Texas, 1155 Union Cir, Denton, TX 76203, USA. LA - eng PT - Journal Article DEP - 20261009 PL - Switzerland TA - Sensors (Basel) JT - Sensors (Basel, Switzerland) JID - 101204366 SB - IM PMC - PMC13708768 OTO - NOTNLM OT - Stark effect OT - molecular gas detection OT - molecular rotational levels COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:47 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - s26206363 [pii] AID - sensors-26-06363 [pii] AID - 10.3390/s26206363 [doi] PST - epublish SO - Sensors (Basel). 2026 Oct 9;26(20):6363. doi: 10.3390/s26206363. PMID- 42944216 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 21 TI - Machine Learning in Mammals' Health: Integrating Multi-Omics Data for Precision Nutrition and Sustainable Production. LID - 10.3390/vetsci13101122 [doi] LID - 1122 AB - Precision nutrition in modern mammalian production aims to transition from static feeding regimens toward dynamic and customized strategies by unraveling complex interactions among genetics, environment, the gut microbiome, and host physiology. Machine learning provides a powerful analytical framework to navigate high-dimensional multi-omics datasets. However, existing applications frequently lack systematic criteria for algorithm selection and remain largely confined to observational statistical associations without progressing toward causal verification. This perspective delineates a coherent translational chain spanning three interconnected operational scenarios: key functional microbiota screening to identify whom to intervene on, multi-omics biomarker identification to define when to intervene, and host pathway characterization to reveal how the animal responds. We critically examine the operational boundaries, interpretability trade-offs, and failure modes of classical algorithms including LASSO, support vector machines, random forest, and graph neural networks across these domains. Furthermore, we address critical field bottlenecks such as high dimensionality, small-sample overfitting, batch effects, and black-box opacity, while highlighting emerging paradigms including multimodal data fusion, transfer learning, and causal machine learning to bridge the gap between data-driven screening and actionable nutritional interventions. Ultimately, transforming machine learning from an auxiliary predictive tool into an autonomous precision nutrition engine requires shifting the research focus from isolated model accuracy to reproducible and causally validated biological targets. FAU - Huang, Weihao AU - Huang W AUID- ORCID: 0009-0002-0877-6156 AD - Animal Nutrition and Bio-Feed, Key Laboratory of Chongqing Education Commission of China, Southwest University, Chongqing 400715, China. FAU - Yu, Xudong AU - Yu X AD - Animal Nutrition and Bio-Feed, Key Laboratory of Chongqing Education Commission of China, Southwest University, Chongqing 400715, China. FAU - Xia, Yaoyao AU - Xia Y AD - Animal Nutrition and Bio-Feed, Key Laboratory of Chongqing Education Commission of China, Southwest University, Chongqing 400715, China. FAU - Li, Weijian AU - Li W AUID- ORCID: 0000-0002-8839-9110 AD - Animal Nutrition and Bio-Feed, Key Laboratory of Chongqing Education Commission of China, Southwest University, Chongqing 400715, China. FAU - Zhao, Xuan AU - Zhao X AD - Animal Nutrition and Bio-Feed, Key Laboratory of Chongqing Education Commission of China, Southwest University, Chongqing 400715, China. LA - eng GR - CSTB2023NSCQ-MSX0786/Natural Science Foundation of Chongqing/ GR - KJQN202500214/Chongqing Municipality Education Commission/ GR - NW-CSTB2025NYKJGGXM-DPJSLXMX0003/Agricultural Science and Technology R&D Program of Chongqing/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709798 OTO - NOTNLM OT - biomarker identification OT - intestinal health OT - intestinal microbiota OT - machine learning OT - precision nutrition COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:47 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - vetsci13101122 [pii] AID - vetsci-13-01122 [pii] AID - 10.3390/vetsci13101122 [doi] PST - epublish SO - Vet Sci. 2026 Oct 21;13(10):1122. doi: 10.3390/vetsci13101122. PMID- 42944207 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 18 TI - Implementation of African Swine Fever Biosecurity and Post-Outbreak Recovery in Vietnam, Malaysia, and the Philippines. LID - 10.3390/vetsci13101113 [doi] LID - 1113 AB - African swine fever (ASF) continues to disrupt pig production in Southeast Asia. Control measures and conditions for recovery vary across production systems. This qualitative multiple-case study examined 24 purposively selected production units in Vietnam (n = 8), Malaysia (n = 12), and the Philippines (n = 4). Government and partner institutions facilitated access to the units. Face-to-face interviews and field notes collected between August 2025 and February 2026 were analysed alongside policy documents. National statistics described changes in pig inventories and output. In Vietnam, live-weight pig output in 2024 exceeded the 2018 level, although the national herd remained smaller. Recovery varied among the sampled units, with greater structural investment in integrated enterprises than in family farms. In Malaysia, nine sampled farms were operating, several were undergoing substantial upgrades, and three remained depopulated while awaiting approval to restock. In the Philippines, one commercial unit reported coordinated control measures and household measures. The three programme-linked backyard units relied on household measures and project support. Gaps in transport, reporting, disposal, and restocking arrangements limited the reach of farm-level measures across the cases. FAU - Duan, Keyi AU - Duan K AUID- ORCID: 0000-0002-9812-820X AD - Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China. FAU - Lin, Ting AU - Lin T AUID- ORCID: 0009-0003-4364-5773 AD - College of Economics and Management, China Agricultural University, Beijing 100083, China. FAU - Zhu, Zengyong AU - Zhu Z AUID- ORCID: 0000-0003-1976-795X AD - Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China. FAU - Rinzin, Karma AU - Rinzin K AD - World Organisation for Animal Health, Sub-Regional Representation for South-East Asia, Bangkok 10400, Thailand. FAU - Sutar, Ashish AU - Sutar A AUID- ORCID: 0009-0003-7528-7480 AD - World Organisation for Animal Health, Sub-Regional Representation for South-East Asia, Bangkok 10400, Thailand. FAU - Charoenlarp, Waroonsiri AU - Charoenlarp W AD - World Organisation for Animal Health, Sub-Regional Representation for South-East Asia, Bangkok 10400, Thailand. LA - eng GR - CAAS-ASTIP-2026-IAS/World Organisation for Animal Health/ GR - World Organisation for Animal Health (WOAH)/ PT - Journal Article DEP - 20261018 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709924 OTO - NOTNLM OT - African swine fever OT - biosecurity OT - farm recovery OT - pig production OT - policy OT - restocking COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:47 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - vetsci13101113 [pii] AID - vetsci-13-01113 [pii] AID - 10.3390/vetsci13101113 [doi] PST - epublish SO - Vet Sci. 2026 Oct 18;13(10):1113. doi: 10.3390/vetsci13101113. PMID- 42944204 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 17 TI - Prostaglandin-Endoperoxide Synthase 2 (PTGS2) Expression Is Increased in Chronic Asymptomatic Orchitis in the Dog. LID - 10.3390/vetsci13101110 [doi] LID - 1110 AB - Chronic asymptomatic orchitis (CAO) in dogs lacks effective treatment options. To elucidate the involvement of prostaglandin endoperoxide synthase 2 (PTGS2), associated with inflammation and physiological steroidogenesis, testicular PTGS2 and StAR gene (via RT-qPCR) and protein expression (via immunohistochemistry) were analyzed in 11 canine CAO samples and compared to five normospermic control group (CG) samples. The percentage of immunopositive area (PIA) and mean gray scale (MGS) intensity were evaluated in the interstitial and tubular compartments. In CAO, PTGS2 and StAR gene expression levels were significantly increased compared to CG (p < 0.001 and p = 0.0034). Leydig cells and some peritubular cells stained immunopositive for PTGS2 and StAR and Sertoli cells positive for PTGS2. PIA and MGS for PTGS2 differed significantly between groups in the interstitial (PIA: p < 0.0001; MGS: p = 0.0051) and tubular (PIA: p = 0.0006; MGS: p = 0.0413) compartments. For StAR, PIA, but not MGS, was significantly higher in CAO (p = 0.0010). Except for PTGS2 gene expression, higher degrees of spermatogenetic impairment correlated with more prominent expression changes. This study demonstrates an association between increased PTGS2 expression and CAO. Further research is needed to investigate potential clinical benefits of selective PTGS2 inhibitors. FAU - Packeiser, Eva-Maria AU - Packeiser EM AUID- ORCID: 0000-0001-6694-3851 AD - Unit for Reproductive Medicine, Clinic for Small Animals, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, Germany. FAU - Korber, Hanna AU - Korber H AD - Unit for Reproductive Medicine, Clinic for Small Animals, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, Germany. FAU - Morawietz, Judith AU - Morawietz J AD - Unit for Reproductive Medicine, Clinic for Small Animals, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, Germany. FAU - Goericke-Pesch, Sandra AU - Goericke-Pesch S AUID- ORCID: 0000-0002-4327-9780 AD - Unit for Reproductive Medicine, Clinic for Small Animals, University of Veterinary Medicine Hannover, Foundation, 30559 Hannover, Germany. LA - eng GR - Gesellschaft fur kynologische Forschung/ PT - Journal Article DEP - 20261017 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709730 OTO - NOTNLM OT - PTGS2 OT - StAR OT - chronic asymptomatic orchitis OT - dog OT - infertility OT - inflammation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:47 PHST- 2026/09/09 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - vetsci13101110 [pii] AID - vetsci-13-01110 [pii] AID - 10.3390/vetsci13101110 [doi] PST - epublish SO - Vet Sci. 2026 Oct 17;13(10):1110. doi: 10.3390/vetsci13101110. PMID- 42944203 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 17 TI - Serological Evidence of Influenza A Virus Exposure and Reactivity to an H1N1pdm09 Reference Strain in Backyard Pigs in Chile. LID - 10.3390/vetsci13101109 [doi] LID - 1109 AB - This study assessed serological exposure to influenza A virus (IAV) in swine backyard production systems (BPS) across 14 regions of Chile. Sera originated from official surveillance for exotic swine diseases in 2020. Database reconciliation identified 928 sample records linked to 123 distinct registered premises; two empty tubes were excluded from sample-level analyses. Nineteen of 926 evaluable samples were ELISA-positive (2.05%; descriptive 95% confidence interval [CI]: 1.32-3.18%), from six premises (4.88%; 95% CI: 2.25-10.23%). Seven initially doubtful samples were retested, yielding six positive and one negative result. Thirteen of the 19 ELISA-positive samples had sufficient residual serum for hemagglutination inhibition (HI) testing; all 13 reacted to the H1N1pdm09 reference strain (titers, 1/40-1/640). Positive premises were located in O'Higgins, Biobio, and Nuble. The results support IAV exposure and HI reactivity to an H1N1pdm09 reference strain, but do not identify the infecting genotype, active circulation, or direction of transmission. Surveillance-based selection and within-premises clustering limit population-level inference. FAU - Ramirez-Toloza, Galia AU - Ramirez-Toloza G AUID- ORCID: 0000-0001-8415-7918 AD - Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago 8820808, Chile. FAU - Ariyama, Naomi AU - Ariyama N AUID- ORCID: 0000-0002-4713-5979 AD - Facultad de Medicina Veterinaria y Agronomia, Universidad de Las Americas, Santiago 7500975, Chile. FAU - Urzua, Constanza AU - Urzua C AD - Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago 8820808, Chile. FAU - Alegria, Raul AU - Alegria R AUID- ORCID: 0000-0002-1608-2499 AD - Escuela de Medicina Veterinaria, Facultad de Medicina, Facultad de Ciencias Biologicas y Facultad de Agronomia y Sistemas Naturales, Pontificia Universidad Catolica de Chile, Santiago 7820436, Chile. FAU - Bennett, Benjamin AU - Bennett B AD - Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago 8820808, Chile. FAU - Perez, Patricio AU - Perez P AD - Servicio Agricola y Ganadero (SAG), Santiago 8320000, Chile. FAU - Pardo-Roa, Catalina AU - Pardo-Roa C AUID- ORCID: 0000-0002-5409-225X AD - Department of Child and Adolescent Health, School of Nursing, Pontificia Universidad Catolica de Chile, Santiago 7820436, Chile. AD - Departamento de Enfermedades Infecciosas e Inmunologia Pediatrica, Escuela de Medicina, Pontificia Universidad Catolica de Chile, Santiago 8330024, Chile. FAU - Medina, Rafael AU - Medina R AD - Department of Child and Adolescent Health, School of Nursing, Pontificia Universidad Catolica de Chile, Santiago 7820436, Chile. AD - Departamento de Enfermedades Infecciosas e Inmunologia Pediatrica, Escuela de Medicina, Pontificia Universidad Catolica de Chile, Santiago 8330024, Chile. AD - Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York City, NY 10029, USA. AD - Department of Pathology and Laboratory Medicine, School of Medicine, Emory Vaccine Center, Emory University, Atlanta, GA 30329, USA. FAU - Neira, Victor AU - Neira V AUID- ORCID: 0000-0001-9062-9969 AD - Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santiago 8820808, Chile. LA - eng GR - HHSN272201400008C/OD/NIH HHS/United States GR - contract No. HHSN272201400008C/the Center for Research in Influenza Pathogenesis (CRIP)/ GR - Proyecto ANID Anillo [ATE 250053]/Agencia Nacional de Investigacion y Desarrollo/ GR - Programa Fondecyt [1261095]/Chilean National Fund for Scientific and Technological Development/ PT - Journal Article DEP - 20261017 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709903 OTO - NOTNLM OT - Chile OT - H1N1pdm09 OT - backyard pigs OT - hemagglutination inhibition OT - influenza A virus OT - serology COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:47 PHST- 2026/08/23 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - vetsci13101109 [pii] AID - vetsci-13-01109 [pii] AID - 10.3390/vetsci13101109 [doi] PST - epublish SO - Vet Sci. 2026 Oct 17;13(10):1109. doi: 10.3390/vetsci13101109. PMID- 42944199 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 16 TI - Seroprevalence of Peste des Petits Ruminants Virus Antibodies and Associated Factors in Small Ruminants Sampled Around Protected Wildlife Areas in Pakistan. LID - 10.3390/vetsci13101105 [doi] LID - 1105 AB - Peste des Petits Ruminants (PPR) threatens small-ruminant production in Pakistan, particularly where livestock grazing overlaps with wildlife habitats. In this exploratory cross-sectional study (January-June 2024), 1939 sera from goats (n = 1327) and sheep (n = 612) in 56 herds, purposively selected near eight protected wildlife areas, were tested for antibodies against PPR virus (PPRV) using a competitive ELISA. Vaccinated and unvaccinated animals were included, and the assay cannot distinguish vaccine-induced from infection-induced antibodies. The overall apparent antibody seroprevalence was 38.5% (747/1939; 95% CI: 36.4-40.7%) (naive 95% CI; it ignores clustering and understates uncertainty, and between-site heterogeneity was very high, I(2) = 94%), ranging from 16.7% in Peshawar to 73.0% at Kirthar National Park, Sindh. Crude odds of seropositivity were higher in goats than in sheep (44.8% vs. 25.0%; OR = 2.43, 95% CI: 1.97-3.01), in animals older than three years than in those aged > 1-3 years (52.0% vs. 33.8%; OR = 2.13, 95% CI: 1.69-2.67), and in herds with more than 100 animals than in herds with 50-100 animals (43.3% vs. 35.7%; OR = 1.38, 95% CI: 1.11-1.72). After adjustment for site, goats remained more likely to be seropositive than sheep (OR = 2.19, 95% CI: 1.76-2.74), although the species difference varied between sites (heterogeneity p = 0.005). Herd-level clustering could not be modelled; associations with herd-level factors (herd size, vaccination status) were sensitive to plausible within-herd correlation and are exploratory. Vaccination categories are reported descriptively only. PPRV antibodies were common in small ruminants sampled near the selected protected areas. However, wildlife were not sampled, distance categories were confounded with site, and results are not nationally representative; transmission at the livestock-wildlife interface was not demonstrated. Targeted surveillance and integrated livestock-wildlife studies are warranted. FAU - Kabir, Abdul AU - Kabir A AD - Department of Veterinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam 70060, Pakistan. FAU - Abubakar, Muhammad AU - Abubakar M AUID- ORCID: 0009-0006-1975-7873 AD - National Veterinary Laboratory, Park Road, Islamabad 44000, Pakistan. FAU - Kamboh, Asghar Ali AU - Kamboh AA AUID- ORCID: 0000-0002-5176-6685 AD - Department of Veterinary Microbiology, Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tandojam 70060, Pakistan. FAU - Manzoor, Shumaila AU - Manzoor S AUID- ORCID: 0000-0002-3623-9086 AD - National Veterinary Laboratory, Park Road, Islamabad 44000, Pakistan. FAU - Farooq, Muhammad AU - Farooq M AD - Health Research Innovation Center, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada. FAU - Matar, Abdulkareem Mohammed AU - Matar AM AUID- ORCID: 0000-0003-0343-5969 AD - Department of Animal Production, College of Food and Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi Arabia. LA - eng GR - (ORF-2026-1111)/King Saud University/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709470 OTO - NOTNLM OT - Peste des Petits Ruminants OT - livestock-wildlife interface OT - seroprevalence OT - small ruminants COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:47 PHST- 2026/09/20 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - vetsci13101105 [pii] AID - vetsci-13-01105 [pii] AID - 10.3390/vetsci13101105 [doi] PST - epublish SO - Vet Sci. 2026 Oct 16;13(10):1105. doi: 10.3390/vetsci13101105. PMID- 42944193 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 16 TI - Comparative Analysis of the Fatty Acid Profiles of Hypoallergenic Veterinary Diets, Commercial Dog Foods, and Oral Fatty Acid Supplements Available on the Hungarian Market. LID - 10.3390/vetsci13101099 [doi] LID - 1099 AB - Polyunsaturated fatty acids (PUFAs), particularly the long-chain n-3 PUFAs (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)), are integral to the dietary management of canine atopic dermatitis (CAD). However, the fatty acid profiles of commercially available canine products on Hungarian markets have not been systematically characterised, and the present study aimed to address this gap. A total of 35 products selected from those available on the Hungarian market at the time of sampling were analysed by gas chromatography with flame ionisation detection (GC-FID): 8 hypoallergenic veterinary diets (HVD), 22 commercial dog foods (CD), and 5 oral fatty acid supplements (FS). FS1-FS4 are complementary feeds under Regulation (EC) No 767/2009. FS5 is a food supplement under Directive 2002/46/EC. FS5 was included for comparative reference only. Fatty acid composition is expressed as % of total peak area. The n-6/n-3 ratio differed substantially across categories (HVD mean 6.16, range 2.65-10.71; CD mean 9.94, range 2.68-18.16; FS1-FS4 mean 18.08, range 0.29-63.49 (FS5 excluded as human product)). Combined % of EPA + DHA was lower in dietary products (HVD mean 0.94%, range 0.16-2.10%; CD mean 0.19%, range 0.09-0.34%) than in marine-based supplements (FS3 7.52%, FS4 24.19%). Alpha-linolenic acid (ALA) was the predominant n-3 fatty acid in 29 of 30 dietary products. Pronounced inter-product heterogeneity was observed among supplements, including one plant-based product with an n-6/n-3 ratio of 63.49. These findings indicate that commercial and hypoallergenic diets contain low proportion of EPA, and that marine-based oral supplementation provided substantially higher proportions of EPA and DHA than any dietary product evaluated. FAU - Toth, Zsombor AU - Toth Z AUID- ORCID: 0009-0001-7147-9026 AD - Department of Internal Medicine, University of Veterinary Medicine, Istvan u. 2, H-1078 Budapest, Hungary. FAU - Febel, Hedvig AU - Febel H AUID- ORCID: 0000-0002-9867-0664 AD - Department of Obstetrics and Food Animal Medicine Clinic, University of Veterinary Medicine, Istvan u. 2, H-1078 Budapest, Hungary. FAU - Wagner, Laszlo AU - Wagner L AD - Department of Animal Nutrition and Nutritional Physiology, Georgikon Campus, Hungarian University of Agriculture and Life Sciences (MATE), Deak Ferenc u. 16, H-8360 Keszthely, Hungary. FAU - Buranszki, Barbara AU - Buranszki B AD - Pillevet Veterinary Practice, Dozsa Gyorgy ut 114, H-2134 Szod, Hungary. FAU - Lajos, Andrea AU - Lajos A AD - Pillevet Veterinary Practice, Dozsa Gyorgy ut 114, H-2134 Szod, Hungary. FAU - Vida, Kata AU - Vida K AD - Diosdvet Veterinary Practice, Sashegyi ut 10, H-2049 Diosd, Hungary. FAU - Tarpataki, Noemi AU - Tarpataki N AD - Department of Internal Medicine, University of Veterinary Medicine, Istvan u. 2, H-1078 Budapest, Hungary. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709901 OTO - NOTNLM OT - canine atopic dermatitis OT - commercial dog food OT - docosahexaenoic acid OT - eicosapentaenoic acid OT - fatty acid supplement OT - gas chromatography OT - hypoallergenic diet OT - n-6/n-3 ratio OT - omega-3 fatty acids COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:47 PHST- 2026/09/12 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - vetsci13101099 [pii] AID - vetsci-13-01099 [pii] AID - 10.3390/vetsci13101099 [doi] PST - epublish SO - Vet Sci. 2026 Oct 16;13(10):1099. doi: 10.3390/vetsci13101099. PMID- 42944189 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 15 TI - Development and Preliminary Validation of a Recombinant DP238L-Based Indirect ELISA for the Serological Detection of African Swine Fever Virus Antibodies. LID - 10.3390/vetsci13101095 [doi] LID - 1095 AB - African swine fever virus (ASFV) antibody detection is an important component of serological surveillance and retrospective investigations. DP238L was recently identified by proteome-wide serum-antibody profiling as a frequently recognized ASFV antigen, and recombinant DP238L was further shown to react with ASFV-positive sera and to elicit antigen-specific antibody responses in immunized pigs. Here, we extend those observations toward diagnostic assay development and preliminary validation. Full-length DP238L from ASFV strain SY-1 (GenBank OM161110) was evaluated as a single recombinant coating antigen for an indirect ELISA (DP238L-iELISA). Independent sequence analysis showed that SY-1 DP238L contains 238 amino acids, with a theoretical molecular mass of 27.13 kDa, a theoretical pI of 4.92, and a GRAVY value of -0.97. In a quality-restricted subset of complete, unambiguous 238-aa homologs (n = 7), amino acid identity to SY-1 ranged from 97.48% to 100% (median, 98.74%). Recombinant DP238L was expressed and purified from Escherichia coli and specifically recognized by ASFV-positive swine serum. ELISA optimization identified a coating antigen concentration of 0.5 mug/mL, a secondary-antibody working dilution of 1:2500 (0.4 mug/mL), a serum dilution of 1:100, a serum incubation time of 45 min, and a substrate development time of 15 min. Using 518 clinical swine serum samples categorized according to the commercial INgezim PPA COMPAC K3 blocking ELISA, ROC analysis yielded an AUC of 0.9989 (bootstrap 95% CI, 0.9973-0.9999). At the selected S/P cutoff of 0.30, positive percent agreement and negative percent agreement relative to INgezim were 97.2% and 98.7%, respectively; overall agreement was 98.3% and Cohen's kappa was 0.956. A limited six-sample heterologous panel provided preliminary cross-reactivity information, with all tested samples remaining below the cutoff. In a longitudinal panel from five experimentally infected pigs, three animals exceeded the cutoff at 7 days post-infection (dpi) and all five exceeded it by 10 dpi. These findings support DP238L as a promising recombinant antigen for ASFV serological assay development while emphasizing that the present results represent an internal comparator-based preliminary evaluation requiring broader independent validation. FAU - Zhang, Yuanfeng AU - Zhang Y AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. FAU - Xiao, Yong AU - Xiao Y AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. FAU - Jiang, Yongjie AU - Jiang Y AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. FAU - Wang, Yongqi AU - Wang Y AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. FAU - Chen, Jing AU - Chen J AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. FAU - Xiang, Zhida AU - Xiang Z AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. FAU - Kang, Chao AU - Kang C AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. FAU - Jin, Meilin AU - Jin M AD - State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China. AD - College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. AD - Hubei Jiangxia Laboratory, Wuhan 430200, China. LA - eng GR - 2025YFD1800700/The National Key Research and Development Program of China/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709942 OTO - NOTNLM OT - African swine fever virus OT - DP238L OT - assay validation OT - indirect ELISA OT - recombinant antigen OT - serology COIS- The authors declare that they have no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:47 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - vetsci13101095 [pii] AID - vetsci-13-01095 [pii] AID - 10.3390/vetsci13101095 [doi] PST - epublish SO - Vet Sci. 2026 Oct 15;13(10):1095. doi: 10.3390/vetsci13101095. PMID- 42944182 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 14 TI - Neuromedin B Regulates Cell Proliferation, Apoptosis and Steroidogenesis of Porcine Ovarian Granulosa Cells via NMBR. LID - 10.3390/vetsci13101088 [doi] LID - 1088 AB - Granulosa cells (GCs) critically govern follicular development through the coordinated regulation of proliferation, apoptosis, and steroidogenesis. Neuromedin B (NMB), a local regulatory peptide, has been implicated in female reproductive processes. However, its precise effects on porcine GCs remain to be elucidated. In this study, we characterized the ovarian distribution of NMBR, investigated the effects of NMB on GC function, and further explored the involvement of NMBR in these effects using the NMBR antagonist PD168368. The results showed that higher NMBR expression was observed in follicles of larger diameter. NMB treatment differentially regulated the functional activities of porcine ovarian GCs. Specifically, certain concentrations of NMB significantly increased cell viability and altered the expression levels of the proliferation-related markers Cyclin B1 and PCNA; reduced the cell apoptosis rate and modulated the expression levels of apoptosis-related genes and proteins (Bax, Bcl-2, and Caspase-3); and affected steroidogenic responses, including increased E(2) concentrations at selected concentrations while altering expression of STAR, CYP11A1, HSD3B1, and CYP19A1. Importantly, several of these effects were attenuated by PD168368 treatment. Collectively, these findings suggest that NMB may regulate multiple functions of porcine with the involvement of NMBR. These findings provide a theoretical basis for elucidating the neuroendocrine mechanisms underlying porcine follicular development and for further investigating the potential role of NMB/NMBR signaling in porcine follicular physiology. FAU - Ma, Zhiyu AU - Ma Z AUID- ORCID: 0009-0001-5934-4700 AD - College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. AD - Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China. FAU - Li, Hongxia AU - Li H AD - College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. FAU - Chen, Guangzhen AU - Chen G AD - College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. FAU - Li, Jia AU - Li J AD - College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. FAU - Wang, Dandan AU - Wang D AD - College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. FAU - Wang, Yiwen AU - Wang Y AD - College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. FAU - Yang, Sheng AU - Yang S AD - College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. AD - Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China. FAU - Liu, Qi AU - Liu Q AD - College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. AD - Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China. FAU - Chen, Fenglei AU - Chen F AUID- ORCID: 0000-0002-7433-5474 AD - College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. AD - Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China. FAU - Pan, Shifeng AU - Pan S AD - College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. AD - Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China. LA - eng GR - 31802149/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709793 OTO - NOTNLM OT - NMB OT - NMBR OT - apoptosis OT - granulosa cells (GCs) OT - porcine OT - proliferation OT - steroidogenesis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:47 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - vetsci13101088 [pii] AID - vetsci-13-01088 [pii] AID - 10.3390/vetsci13101088 [doi] PST - epublish SO - Vet Sci. 2026 Oct 14;13(10):1088. doi: 10.3390/vetsci13101088. PMID- 42944179 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 13 TI - Dietary Curcumin Protects Common Carp (Cyprinus carpio) Against Potassium Permanganate-Induced Toxicity: Hematological, Immunological, Oxidative, and Molecular Docking Evidence. LID - 10.3390/vetsci13101085 [doi] LID - 1085 AB - Potassium permanganate treatment can adversely affect fish physiology despite its usefulness in aquaculture. This study evaluated the protective effects of dietary curcumin against potassium permanganate-induced disturbances in common carp (Cyprinus carpio). Fish were fed a basal diet or diets supplemented with 1%, 2%, or 4% curcumin for eight weeks, with or without subsequent exposure to potassium permanganate at 2 mg/L for 30 min daily for seven days. Hematological and immune parameters and oxidative status in the liver, kidney, spleen, and gill were assessed. Potassium permanganate exposure altered blood and immune parameters, increased lipid peroxidation, and weakened antioxidant defenses. Curcumin supplementation alleviated these effects, with protection evident at 1%, the lowest dietary inclusion level tested, and further improvements in selected parameters at 4%. Inclusion levels below 1% were not evaluated, so the minimum effective level remains unknown. Molecular docking predicted interactions between curcumin and the antioxidant enzymes SOD, CAT, GPx, and GST at structurally plausible binding sites. These computational findings were interpreted alongside the biological results as supportive, hypothesis-generating evidence rather than confirmation of functional effects on these enzymes in vivo. Overall, dietary curcumin showed potential to reduce the physiological disturbances associated with repeated potassium permanganate exposure. Further dose-response studies should evaluate inclusion levels below 1% to identify effective and economically viable supplementation rates and determine whether curcumin affects the antiparasitic or antibacterial efficacy of potassium permanganate. FAU - Eroglu, Mucahit AU - Eroglu M AUID- ORCID: 0000-0002-1775-1201 AD - Department of Basic Aquatic Sciences, Faculty of Fisheries, Firat University, Elazig 23200, Turkiye. FAU - Mise Yonar, Serpil AU - Mise Yonar S AD - Department of Aquaculture, Faculty of Fisheries, Firat University, Elazig 23200, Turkiye. FAU - Tanrikulu, Duygu AU - Tanrikulu D AD - Department of Biology, Faculty of Arts and Sciences, Kafkas University, Kars 36100, Turkiye. FAU - Uslu, Harun AU - Uslu H AUID- ORCID: 0000-0001-8827-8557 AD - Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Firat University, Elazig 23200, Turkiye. FAU - Yonar, Muhammet Enis AU - Yonar ME AUID- ORCID: 0000-0001-9519-4247 AD - Department of Aquaculture, Faculty of Fisheries, Firat University, Elazig 23200, Turkiye. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709893 OTO - NOTNLM OT - curcumin OT - fish OT - hematology OT - immunity OT - molecular docking OT - oxidative stress OT - potassium permanganate COIS- There are no conflicts of interest that would compromise the impartiality of the reported research. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:47 PHST- 2026/09/21 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - vetsci13101085 [pii] AID - vetsci-13-01085 [pii] AID - 10.3390/vetsci13101085 [doi] PST - epublish SO - Vet Sci. 2026 Oct 13;13(10):1085. doi: 10.3390/vetsci13101085. PMID- 42944176 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 13 TI - Exploratory Evaluation of the P22 ELISA for Antibody Detection in Fecal Samples from Tuberculosis-Infected Goat Herds. LID - 10.3390/vetsci13101083 [doi] LID - 1083 AB - Background: Caprine tuberculosis (cTB), caused by members of the Mycobacterium tuberculosis complex (MTBC) represents a significant concern for animal and public health. Antibody-based diagnostic tests have emerged as promising tools to complement ante-mortem official diagnostic techniques in ruminants, which mainly use serum, although alternative, easier-to-collect samples, such as milk, oral fluid, or feces, could also be used. The objective of this exploratory study was to evaluate the reactivity of an in-house P22 enzyme-linked immunosorbent assay (ELISA) in fecal samples from goats belonging to cTB-infected herds, using serum P22 ELISA and official diagnostic tests as comparators. Methods: Serum and fecal samples were collected from 51 and 42 adult female goats from a high-prevalence herd (n = 149) and a low-prevalence herd (n = 220), respectively, which defecated during sampling. These animals were subjected to official diagnostic techniques: single and comparative intradermal tuberculin tests and the interferon-gamma release assay. As no TB-free herd was available, a preliminary fecal cut-off was established by receiver operating characteristic analysis using serum P22 ELISA as the reference classification. Results: The percentage of positive animals in fecal samples from the high-prevalence herd was 68.6%, while no positive results were observed in the low-prevalence herd. Serum samples showed a higher number of positives in both herds compared to fecal samples (p < 0.05). The agreement between official diagnostic techniques and P22 ELISA (using serum or feces) was slight in general terms. When a combined classification based on all official tests was used as the reference, fecal P22 ELISA detected 58.0% of test-positive goats, none of them in the low-prevalence herd. Conclusions: Under the conditions of this field study (no TB-free controls or post-mortem confirmation of infection were available), fecal P22 ELISA showed limited reactivity compared with serum, particularly in the low-prevalence herd, suggesting limited usefulness for antibody detection in TB infected goats. FAU - Ortega, Javier AU - Ortega J AD - VISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, Spain. FAU - Infantes-Lorenzo, Jose A AU - Infantes-Lorenzo JA AUID- ORCID: 0000-0002-0446-7682 AD - Reference and Research Laboratory for Respiratory Virus, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, 28040 Madrid, Spain. FAU - Gomez-Buendia, Alberto AU - Gomez-Buendia A AUID- ORCID: 0000-0002-8968-0836 AD - VISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, Spain. AD - Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain. FAU - Roy, Alvaro AU - Roy A AUID- ORCID: 0000-0002-1436-8012 AD - Centro Nacional de Epidemiologia, Instituto de Salud Carlos III, 28040 Madrid, Spain. FAU - Velasco, Carlos AU - Velasco C AUID- ORCID: 0000-0003-0190-4581 AD - VISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, Spain. AD - Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain. FAU - Romero, Beatriz AU - Romero B AD - VISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, Spain. AD - Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain. FAU - Moreno, Inmaculada AU - Moreno I AUID- ORCID: 0000-0001-5001-3764 AD - Unidad de Inmunologia Microbiana, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, 28040 Madrid, Spain. FAU - Dominguez, Mercedes AU - Dominguez M AD - Unidad de Inmunologia Microbiana, Centro Nacional de Microbiologia, Instituto de Salud Carlos III, Majadahonda, 28040 Madrid, Spain. FAU - Bezos, Javier AU - Bezos J AUID- ORCID: 0000-0003-1913-0545 AD - VISAVET Health Surveillance Centre, Complutense University of Madrid, 28040 Madrid, Spain. AD - Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain. LA - eng GR - PID2024-159293OB-I00/Ministerio de Ciencia, Innovacion y Universidades/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709756 OTO - NOTNLM OT - P22 ELISA OT - antibodies OT - caprine tuberculosis OT - diagnosis OT - fecal samples OT - goats OT - non-invasive sampling COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:47 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - vetsci13101083 [pii] AID - vetsci-13-01083 [pii] AID - 10.3390/vetsci13101083 [doi] PST - epublish SO - Vet Sci. 2026 Oct 13;13(10):1083. doi: 10.3390/vetsci13101083. PMID- 42944174 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 12 TI - A Review on Parrot Gut Health: Insights into the Intestinal Structure, Microbiota Diversity, Influencing Factors, and Nutritional Strategies. LID - 10.3390/vetsci13101079 [doi] LID - 1079 AB - The parrots, a highly intelligent avian species, possesses distinctive characteristics including remarkable cognitive abilities, strong mimetic capacity, and significant economic value as companion pets, with the scale of commercial captive breeding expanding considerably over recent decades. Intestinal health is critical for nutrient digestion and absorption, and serves as a primary barrier against pathogen invasion; thus, maintaining optimal gut function is essential for enhancing overall health and breeding performance. However, compared to other poultry species, research on the pivotal role of gut health in the breeding and management of parrots remains limited. This review summarizes the unique anatomical and physiological features of the parrot digestive system, the characteristics of its gut microbiota, and their interrelationship with intestinal health, and further discusses the significance of intestinal health for the overall well-being of parrots. Additionally, this review highlights emerging nutritional regulation strategies, particularly the application of probiotics and other functional additives for improving intestinal health, which will provide new insights into enhancing the health status of parrot populations and formulating management strategies for the parrot breeding industry. FAU - Jia, Yan AU - Jia Y AD - School of Animal Engineering, Xuzhou Vocational College of Bioengineering, Xuzhou 221006, China. FAU - Li, Yan AU - Li Y AD - College of Pet Science and Technology, Zhengzhou Urban Construction Vocational College, Zhengzhou 451200, China. FAU - Shaukat, Aftab AU - Shaukat A AUID- ORCID: 0000-0003-1670-6916 AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. FAU - Huang, Shu-Cheng AU - Huang SC AUID- ORCID: 0000-0003-3163-8616 AD - College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China. LA - eng GR - 2025QYSJ056/2025 Jiangsu Provincial Higher Vocational College Teachers Enterprise Practice Training Project/ GR - KC25040/Science and Technology Program of Xuzhou/ PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709899 OTO - NOTNLM OT - One Health OT - bird OT - gut microbiota OT - nutritional regulation OT - pet OT - probiotics OT - trace elements COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:47 PHST- 2026/08/07 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - vetsci13101079 [pii] AID - vetsci-13-01079 [pii] AID - 10.3390/vetsci13101079 [doi] PST - epublish SO - Vet Sci. 2026 Oct 12;13(10):1079. doi: 10.3390/vetsci13101079. PMID- 42944166 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 10 TI - MicroRNA Regulation of the Estrogen Receptor Alpha 3'UTR in Canine Mammary Tumor (CMT28) Cells. LID - 10.3390/vetsci13101071 [doi] LID - 1071 AB - Reduced estrogen receptor alpha (ERalpha/ESR1) expression is seen in canine mammary tumors, but the mechanisms involved are not fully understood. This study examined ESR1, miR-18a, and miR-19a expression in canine mammary cell lines. Further CMT28, a canine mammary tumor cell line, was used to test whether these miRNAs can regulate the reporter activity through selected regions of the canine ESR1 3'UTR containing predicted miR-18a and miR-19a binding sites. ESR1 mRNA expression was measured by RT-qPCR, and miR-18a and miR-19a expression was measured using LNA-based assays. ESR1 mRNA expression was much lower in CMT27, CMT28, and CMT47 compared with normal canine mammary epithelial cells, while CMT12 showed higher expression. miR-18a and miR-19a were increased to different levels across the tumor cell lines. Bioinformatic analysis identified predicted miR-18a and miR-19a seed-match sites in the canine ESR1 3'UTR. Luciferase reporter assays showed that both miR-18a and miR-19a significantly reduced activity of the reporter containing selected regions of the 3'UTR of ESR1 encoding predicted miR-18a and miR-19a binding sites. In contrast, neither miRNA significantly reduced activity of the mutant reporter containing deletions in the predicted miR-18a and miR-19a seed-match regions. Anti-miR-18a and anti-miR-19a also significantly increased reporter activity compared with the corresponding miRNA alone. These findings provide functional evidence that miR-18a and miR-19a can regulate reporter activity through selected regions of the canine ESR1 3'UTR. Further studies are needed to determine the role of this regulation in endogenous ESR1 expression and canine mammary tumor biology. FAU - Goyal, Deepika AU - Goyal D AUID- ORCID: 0000-0001-7295-3239 AD - Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA. FAU - Bird, Richard Curtis AU - Bird RC AD - Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, AL 36849, USA. LA - eng PT - Journal Article DEP - 20261010 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709629 OTO - NOTNLM OT - 3' untranslated region OT - canine mammary tumor OT - estrogen receptor 1 (ESR1) OT - luciferase reporter assay OT - miR-18a OT - miR-19a OT - post-transcriptional regulation COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:47 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - vetsci13101071 [pii] AID - vetsci-13-01071 [pii] AID - 10.3390/vetsci13101071 [doi] PST - epublish SO - Vet Sci. 2026 Oct 10;13(10):1071. doi: 10.3390/vetsci13101071. PMID- 42944159 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 9 TI - Epidermoid Cyst of the Soft Palate in a Geriatric Cat: A Case Report. LID - 10.3390/vetsci13101064 [doi] LID - 1064 AB - Epidermoid cysts within the oral cavity are rare in cats. Here, we report the clinical, imaging, surgical, and histopathological findings of an epidermoid cyst originating from the soft palate of a geriatric cat. A 17-year-old neutered female domestic shorthaired cat presented with mild hypersalivation and dysphagia. Computed tomography revealed a well-circumscribed, minimally contrast-enhancing mass measuring 8.5 x 9.9 x 10.0 mm arising from the right distal soft palate. The mass protruded predominantly into the oropharynx and caused mild nasopharyngeal displacement, without evidence of bone invasion or middle ear involvement. Surgical excision was performed, and histopathological examination revealed a cyst lined with keratinizing stratified squamous epithelium containing keratinaceous debris, which was consistent with a benign epidermoid cyst. Hypersalivation and dysphagia were no longer observed after excision, although this improvement was documented for only approximately 24 h before severe gastrointestinal dysfunction and recurrent seizures developed. Although repeated oral mucosal irritation was considered a possible contributing factor, the congenital or acquired origin of the cyst could not be definitively determined. This case highlights that epidermoid cysts should be considered in the differential diagnosis of cystic lesions of the oral cavity in cats. FAU - Choi, Hyehyun AU - Choi H AD - 24h Jamsil ON Animal Medical Center, Seoul 05556, Republic of Korea. FAU - Kim, Kyung Ah AU - Kim KA AD - Seocho I:UM Animal Hospital, Seoul 06643, Republic of Korea. FAU - Lee, Da Eun AU - Lee DE AD - 24h Jamsil ON Animal Medical Center, Seoul 05556, Republic of Korea. FAU - Lee, Juwon AU - Lee J AD - 24h Jamsil ON Animal Medical Center, Seoul 05556, Republic of Korea. FAU - Kim, Jihun AU - Kim J AD - 24h Jamsil ON Animal Medical Center, Seoul 05556, Republic of Korea. FAU - Jang, Hyomi AU - Jang H AUID- ORCID: 0009-0009-8746-1267 AD - Academic Committee, Korean Society of Feline Medicine (KSFM), Seoul 08531, Republic of Korea. FAU - Jung, Dong-In AU - Jung DI AUID- ORCID: 0000-0002-5116-6006 AD - College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of Korea. LA - eng PT - Case Reports PT - Journal Article DEP - 20261009 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709755 OTO - NOTNLM OT - cat OT - computed tomography OT - epidermoid cyst OT - soft palate OT - surgical excision COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:47 PHST- 2026/08/25 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - vetsci13101064 [pii] AID - vetsci-13-01064 [pii] AID - 10.3390/vetsci13101064 [doi] PST - epublish SO - Vet Sci. 2026 Oct 9;13(10):1064. doi: 10.3390/vetsci13101064. PMID- 42944156 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 9 TI - Effects of Yeast Cell Wall Supplementation in Broiler Chickens Under Dietary Aflatoxin Challenge. LID - 10.3390/vetsci13101061 [doi] LID - 1061 AB - Aflatoxins (AFLAs) are highly prevalent mycotoxins in poultry production and are known to impair immune function and may reduce vaccine responsiveness. Therefore, this study evaluated whether dietary supplementation with yeast cell wall (YCW) could modulate the immune response under AFLA challenge and support vaccine response in broilers. A total of 144 one-day-old chicks were distributed into four treatments: negative control (NC), AFLA-contaminated diet (2.5 ppm), YCW (500 g/t), and YCW + AFLA. Birds were vaccinated against Newcastle Disease Virus (NDV) on D14. Body weight, hepatic pathology, intestinal permeability, circulating immune cell populations, phagocytic activity, and NDV-specific antibody titers were evaluated throughout the experimental period. AFLA exposure induced significant hepatic lesions and reduced body weight on D35 but did not alter the systemic immune parameters or intestinal permeability. Liver lesions were not prevented by the use of YCW, but body weight was positively correlated to the YCW intervention. Interestingly, YCW supplementation modulated immune responses in a context-dependent manner. In birds not exposed to AFLA, YCW increased anti-NDV antibody titers following vaccination. Contrarily, under AFLA challenge (and only in this situation), YCW supplementation was associated with changes in circulating immune cell populations and increased monocyte phagocytic activity, but in the presence of AFLA, YCW did not associate with increased anti-NDV titers. These findings indicate that YCW uniquely modulates selected innate and adaptive immune responses in broiler chickens exposed to AFLA, which is an important biological interaction with relevance to feed formulation for animal production. FAU - Bonato, Melina Aparecida AU - Bonato MA AUID- ORCID: 0000-0003-3697-3464 AD - ICC Animal Nutrition, Jundiai 13211-223, Brazil. FAU - Valini, Graziela Alves da Cunha AU - Valini GADC AD - ICC Animal Nutrition, Jundiai 13211-223, Brazil. FAU - Ingberman, Max AU - Ingberman M AD - Imunova Analise Biologicas, Curitiba 80215-182, Brazil. FAU - Yimam, Tadesse Mihret AU - Yimam TM AUID- ORCID: 0000-0001-6416-6972 AD - Department of Basic Pathology, Universidade Federal do Parana (UFPR), Curitiba 81531-980, Brazil. FAU - Beirao, Breno Castello Branco AU - Beirao BCB AUID- ORCID: 0000-0001-5870-1369 AD - Imunova Analise Biologicas, Curitiba 80215-182, Brazil. AD - Department of Basic Pathology, Universidade Federal do Parana (UFPR), Curitiba 81531-980, Brazil. LA - eng PT - Journal Article DEP - 20261009 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709935 OTO - NOTNLM OT - aflatoxin OT - immune modulation OT - mycotoxin contamination OT - poultry OT - yeast cell wall COIS- The authors declare no conflicts of interest. G.A.C.V. and M.A.B. are employed by ICC Brazil, the company that provided the yeast cell wall product tested in this study, and participated in manuscript writing. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:47 PHST- 2026/08/19 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - vetsci13101061 [pii] AID - vetsci-13-01061 [pii] AID - 10.3390/vetsci13101061 [doi] PST - epublish SO - Vet Sci. 2026 Oct 9;13(10):1061. doi: 10.3390/vetsci13101061. PMID- 42944149 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 8 TI - Canine and Feline Melanoma: Diagnosis, Treatment, and Prognosis. LID - 10.3390/vetsci13101053 [doi] LID - 1053 AB - Melanocytic tumors are clinically important in veterinary oncology because their biological behavior, diagnosis, and prognosis vary substantially by species and anatomical site. Canine oral melanoma is characterized by aggressive local invasion and early metastatic spread, whereas melanocytic tumors at other canine sites and in cats show distinct clinicopathologic features that require different diagnostic and therapeutic approaches. This narrative review was based on a structured literature search of PubMed, with searches organized around epidemiology and biological behavior, diagnosis, treatment, and prognosis. A total of 1887 records were identified after removal of duplicates, and 111 publications were ultimately included following title, abstract, and full-text screening. This review summarizes current evidence on the epidemiology, molecular biology, diagnosis, clinical staging, treatment, and prognosis of canine and feline melanocytic neoplasms. Diagnostic approaches include cytology, histopathology, immunohistochemistry for amelanotic tumors, advanced imaging, sentinel lymph node mapping, and emerging molecular methods. Therapeutic options include surgery, radiation therapy, electrochemotherapy, chemotherapy, therapeutic vaccination, immune checkpoint inhibitors, and targeted therapies. Prognostic factors include tumor location and stage, histologic grade, mitotic count, nuclear atypia, pigmentation, tissue invasion, and molecular biomarkers. Surgical excision remains the cornerstone of treatment for localized disease, but effective systemic therapies for advanced melanoma remain limited. Ongoing molecular characterization, genomic profiling, and comparative oncology research may improve prognostic stratification and support more precise therapeutic strategies. FAU - Jeong, Haein AU - Jeong H AUID- ORCID: 0009-0000-0433-7434 AD - College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of Korea. FAU - Shin, Minyeong AU - Shin M AD - College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of Korea. FAU - Kim, Yujin AU - Kim Y AD - College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of Korea. FAU - Ko, Minseo AU - Ko M AD - College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of Korea. FAU - Jung, Dong-In AU - Jung DI AUID- ORCID: 0000-0002-5116-6006 AD - College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of Korea. FAU - Cho, Kyu-Woan AU - Cho KW AD - College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of Korea. FAU - Bae, Hyeona AU - Bae H AUID- ORCID: 0000-0002-2888-5782 AD - College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of Korea. LA - eng GR - RS-2024-00398561/Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries/ GR - GNU-NFRG-0030/Gyeongsang National University/ PT - Journal Article PT - Review DEP - 20261008 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709743 OTO - NOTNLM OT - canine melanoma OT - diagnosis OT - feline melanoma OT - immunohistochemistry OT - immunotherapy OT - melanocytic tumors OT - prognosis OT - radiation therapy OT - sentinel lymph node OT - surgery COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:47 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - vetsci13101053 [pii] AID - vetsci-13-01053 [pii] AID - 10.3390/vetsci13101053 [doi] PST - epublish SO - Vet Sci. 2026 Oct 8;13(10):1053. doi: 10.3390/vetsci13101053. PMID- 42944142 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 6 TI - L-Carnitine Supplementation in Overweight Dogs: An Uncontrolled Pilot Study on Nutrient Digestibility, Metabolic Health, and Body Condition. LID - 10.3390/vetsci13101046 [doi] LID - 1046 AB - BACKGROUND: L-carnitine supports mitochondrial fatty acid transport and has been proposed for weight management, but its short-term effects in overweight dogs are unclear. This pilot study aimed to generate preliminary, hypothesis-generating data on these effects, including possible dose-response patterns, to inform the design of a future randomized, placebo-controlled trial. METHODS: Twenty-one Golden Retriever dogs (8-10 years) were assigned to three groups of seven: a control diet group (BCS < 7) receiving no supplementation, receiving no supplementation, and two overweight groups (BCS >/= 7) receiving 1 g/day or 2 g/day oral L-carnitine for 30 days. Digestibility, faecal dry matter and consistency, BCS, body weight, appetite (4-day monadic feeding test), and serum biochemical, endocrinological and oxidative-status parameters were assessed. RESULTS: BCS, as assessed by a blinded investigator, decreased from baseline at both doses (p < 0.05), more markedly at 2 g/day; however, body weight remained unchanged throughout the study period. Food intake during the post-supplementation appetite test was lower at 2 g/day than in the healthy reference group (p < 0.05), though no pre-supplementation baseline appetite measurement was available for comparison. Serum cholesterol decreased and total antioxidant status (TAS) increased at both doses by day 30 (p < 0.05); however, HDL also decreased at both doses and triglycerides increased at 2 g/day, indicating that lipid profile changes were not uniformly favourable. Insulin decreased at both doses, and visfatin decreased significantly at 2 g/day (p < 0.05). No significant changes in digestibility were observed. CONCLUSIONS: In this uncontrolled pilot study, short-term L-carnitine supplementation was associated with dose-related within-group changes in selected metabolic indicators in overweight dogs, without measurable weight loss within 30 days. Notably, the absence of uniformly favourable lipid profile changes and the lack of an untreated overweight control group limit interpretation. These findings should be regarded as hypothesis-generating and support the rationale for a future placebo-controlled trial, rather than establishing a definitive treatment effect, before L-carnitine can be recommended as an adjunct to longer-term canine obesity-management strategies. FAU - Kahraman, Oguzhan AU - Kahraman O AUID- ORCID: 0000-0002-9315-5276 AD - Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Selcuk University, 42130 Konya, Turkiye. FAU - Inanc, Zekeriya Safa AU - Inanc ZS AUID- ORCID: 0000-0003-0832-9209 AD - Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Selcuk University, 42130 Konya, Turkiye. FAU - Parlak, Tugba Melike AU - Parlak TM AUID- ORCID: 0000-0003-3277-0336 AD - Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Selcuk University, 42130 Konya, Turkiye. FAU - Celik, Metin AU - Celik M AUID- ORCID: 0009-0009-2425-4754 AD - Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Erciyes University, 38039 Kayseri, Turkiye. FAU - Damar, Samed AU - Damar S AD - Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Selcuk University, 42130 Konya, Turkiye. FAU - Dik, Burak AU - Dik B AUID- ORCID: 0000-0003-2738-6911 AD - Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Selcuk University, 42130 Konya, Turkiye. LA - eng PT - Journal Article DEP - 20261006 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709912 OTO - NOTNLM OT - L-carnitine supplementation OT - antioxidant capacity OT - canine obesity OT - digestibility OT - fatty acid metabolism OT - weight management COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/06 CRDT- 2026/10/27 10:47 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/06 00:00 [pmc-release] AID - vetsci13101046 [pii] AID - vetsci-13-01046 [pii] AID - 10.3390/vetsci13101046 [doi] PST - epublish SO - Vet Sci. 2026 Oct 6;13(10):1046. doi: 10.3390/vetsci13101046. PMID- 42944140 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 5 TI - Effects of Eucommia ulmoides Leaf-Astragalus membranaceus Complex on Antioxidant Capacity, Immune Function, Gut Microbiota and Serum Metabolome of Yellow-Feather Broilers. LID - 10.3390/vetsci13101044 [doi] LID - 1044 AB - Background: Phytogenic feed additives may provide sustainable alternatives for improving poultry productivity and health. This study evaluated the chemical composition, antioxidant activity, and in vivo effects of a 1:1 Eucommia ulmoides leaf-Astragalus membranaceus complex (EG) in yellow-feathered broilers. Methods: The nutritional composition, bioactive compounds, and antioxidant activity of EG were characterized. A total of 200 one-day-old yellow-feathered broilers were randomly allocated into four dietary treatments with five replicates per group and 10 birds per replicate: the control group (CG) receiving a basal diet and three EG supplementation groups receiving diets supplemented with 0.5% (EG (0.5%)), 1.0% (EG (1.0%)), 2.0% (EG (2.0%)) Eucommia ulmoides leaf-Astragalus membranaceus complex for 42 days. Growth performance, serum antioxidant and immune indicators, intestinal microbiota composition, and serum metabolomic profiles were analyzed. Results: EG contained 46.53% polysaccharides and exhibited concentration-dependent radical-scavenging activity. Supplementation with EG (0.5%) consistently increased body weight and average daily gain and reduced the feed conversion ratio without affecting feed intake. EG increased serum antioxidant enzyme activities and immunoglobulin concentrations but did not affect routine biochemical parameters. The EG (0.5%) group was therefore selected for microbiome and metabolome analyses. EG altered jejunal and cecal microbial composition without significantly affecting alpha-diversity and increased cecal Faecalibacterium and Blautia at day 21 and Faecalibacterium and Parabacteroides at day 42. Metabolomics identified 103 differential metabolites associated mainly with amino acid, glycerophospholipid, and energy metabolism. Spearman's analysis further associated Bacteroidota, Bacillota, Enterococcus, and Blautia with amino acid, carbohydrate, and lipid-related metabolites. Conclusions: These findings indicate that EG (0.5%) may improve growth and feed efficiency while modulating antioxidant status, humoral immune indices, intestinal microbial composition, and systemic metabolism in yellow-feathered broilers. FAU - Ma, Hongyan AU - Ma H AD - College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China. AD - Department of Animal Science, Shanxi Yuncheng Agricultural Vocational and Technical College, Yuncheng 044000, China. FAU - Yao, Yi AU - Yao Y AD - Department of Animal Science, Shanxi Yuncheng Agricultural Vocational and Technical College, Yuncheng 044000, China. FAU - Wei, Yanming AU - Wei Y AUID- ORCID: 0000-0001-9632-1885 AD - College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China. LA - eng GR - YCKJ-2022057/Yuncheng Science and Technology Program/ PT - Journal Article DEP - 20261005 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709727 OTO - NOTNLM OT - Astragalus membranaceus OT - Eucommia ulmoides leaf OT - antioxidant capacity OT - growth performance OT - gut microbiota OT - herbal feed additive OT - serum metabolomics OT - yellow-feathered broiler COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/05 CRDT- 2026/10/27 10:47 PHST- 2026/08/05 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/05 00:00 [pmc-release] AID - vetsci13101044 [pii] AID - vetsci-13-01044 [pii] AID - 10.3390/vetsci13101044 [doi] PST - epublish SO - Vet Sci. 2026 Oct 5;13(10):1044. doi: 10.3390/vetsci13101044. PMID- 42944136 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 2 TI - Directed Acyclic Graphs for Causal Modeling of Musical Interventions in High-Tropical Grazing Dairy Cattle: A Causal Framework Review. LID - 10.3390/vetsci13101040 [doi] LID - 1040 AB - Musical exposure in dairy cattle has been associated with potential effects on milk production, behavior, and physiological regulation; however, available evidence remains heterogeneous and potentially affected by biological and environmental confounding. In high-tropical grazing systems, these responses are simultaneously influenced by stress physiology, disease, nutrition, management, climatic variability, and individual adaptation. This review integrated causal inference and graphical epidemiology to develop biologically grounded directed acyclic graphs (DAGs) representing hypothesized causal pathways between musical exposure and milk production, behavior, and heart rate variability (HRV). An analytical narrative synthesis of physiological, ethological, autonomic, and epidemiological evidence was conducted, and three independent DAGs were constructed using DAGitty version 3.1. Variables were classified according to their epidemiological roles. The production DAG identified stress as a major integrative node linking disease, nutrition, reproductive physiology, management, and milk yield. The behavioral DAG highlighted the roles of ecological organization, resource access, social interaction, and environmental instability. The HRV DAG treated HRV as beat-to-beat variation in cardiac intervals that provides a non-invasive index of autonomic nervous system (ANS) modulation, including sympathetic and parasympathetic influences, and indicated that responses may occur through indirect modulation of stress physiology and vagal activity. We conclude that musical interventions should be interpreted as context-dependent sensory exposures rather than isolated causal determinants; the proposed DAGs provide an explicit basis for selecting covariates, avoiding inappropriate adjustment, and designing future studies of dairy cattle welfare and production. FAU - Betancourth-Chaves, Patricia AU - Betancourth-Chaves P AD - Research Group Patobiologia Quiron, School of Veterinary Medicine, Agrarian Sciences Faculty, University of Antioquia, Medellin 050036, Colombia. AD - Research Group GIsCA, Faculty of Veterinary Medicine and Animal Sciences, Institucion Universitaria Vision de las Americas, Medellin 050031, Colombia. FAU - Montoya-Zuluaga, John Jairo AU - Montoya-Zuluaga JJ AUID- ORCID: 0000-0001-8554-5971 AD - Research Group GIsCA, Faculty of Veterinary Medicine and Animal Sciences, Institucion Universitaria Vision de las Americas, Medellin 050031, Colombia. FAU - Rodriguez, Berardo de Jesus AU - Rodriguez BJ AUID- ORCID: 0000-0001-9798-0161 AD - Research Group Patobiologia Quiron, School of Veterinary Medicine, Agrarian Sciences Faculty, University of Antioquia, Medellin 050036, Colombia. FAU - Escobar-Riomalo, Jose Eduardo AU - Escobar-Riomalo JE AUID- ORCID: 0009-0006-9490-3543 AD - Research Group GIsCA, Faculty of Veterinary Medicine and Animal Sciences, Institucion Universitaria Vision de las Americas, Medellin 050031, Colombia. FAU - Vallejo-Timaran, Dario Antonio AU - Vallejo-Timaran DA AUID- ORCID: 0000-0002-6682-7743 AD - Obonuco Research and Technology Transfer Center, Corporacion Colombiana de Investigacion Agropecuaria-AGROSAVIA, Pasto 520038, Colombia. LA - eng GR - Grant Number 937 (Fundamental Research) - macro-project "Design of a preventive model based on metabolic profile in the transition period to improve reproductive efficiency in grazing dairy cows in the high tropics"/Ministry of Science, Technology and Innovation/ GR - QUIRON Pathobiology Group and the Committee for the Development of Research (CODI), Uni-versity of Antioquia/Universidad de Antioquia/ PT - Journal Article PT - Review DEP - 20261002 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709351 OTO - NOTNLM OT - animal behavior OT - biomusicology OT - causal inference OT - grazing dairy cattle COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/02 CRDT- 2026/10/27 10:47 PHST- 2026/08/20 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/02 00:00 [pmc-release] AID - vetsci13101040 [pii] AID - vetsci-13-01040 [pii] AID - 10.3390/vetsci13101040 [doi] PST - epublish SO - Vet Sci. 2026 Oct 2;13(10):1040. doi: 10.3390/vetsci13101040. PMID- 42944133 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Oct 1 TI - Meningoencephalitis in Neonatal Calves Caused by Rare O161 Serotype Escherichia coli: A Case Report. LID - 10.3390/vetsci13101037 [doi] LID - 1037 AB - Meningoencephalitis is a rare but fatal form of colibacillosis in neonatal calves. An outbreak of neurological disease characterized by high fever, lethargy, opisthotonus, and death occurred in approximately one-week-old calves on a dairy farm in Xinjiang, China; 13 calves died despite antibiotic therapy. Necropsy revealed severe meningeal hemorrhage and increased cerebrospinal fluid. Five Escherichia coli isolates were obtained from two dead calves (brain-derived CHN-XJ-B1-B4; liver-derived CHN-XJ-L). The isolates carried the K99 and STa virulence genes, and two representative strains were serotyped as the rare O161. All isolates were resistant to 12 of 16 tested antibiotics but susceptible to amikacin, cefazolin, and neomycin. The median lethal doses (LD(50)) in mice were 9.8 x 10(5) CFU (CHN-XJ-B1) and 2.3 x 10(6) CFU (CHN-XJ-L). Histopathology showed cerebral vascular congestion, neuronal degeneration and necrosis, and neutrophilic meningeal infiltration. All isolates harbored the invasion gene ibeB; this gene was nearly identical to that of the non-pathogenic E. coli K-12 strain MG1655 (98.5-100% nucleotide identity) and shared approximately 96% nucleotide identity with the human meningitis strain RS218. Deletion of ibeB significantly reduced bacterial burdens in the liver and spleen and in the brain at 48 h post-infection, increased the LD(50) by approximately tenfold and significantly prolonged mouse survival, indicating that ibeB contributes to systemic dissemination and tissue colonization of the isolates in this model. This is the first report of calf meningoencephalitis caused by O161 E. coli in the international literature. FAU - Ling, Chen AU - Ling C AUID- ORCID: 0000-0002-3888-7933 AD - Tecon Bio-Technology Co., Ltd., Urumqi 830000, China. AD - Engineering and Technology Research Center for Veterinary Biological Products, Xinjiang Production and Construction Corps, Urumqi 830000, China. FAU - Wang, Aohong AU - Wang A AD - College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. FAU - Zhang, Qiwen AU - Zhang Q AUID- ORCID: 0009-0008-4224-582X AD - College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. FAU - Hao, Chengwu AU - Hao C AD - Tecon Bio-Technology Co., Ltd., Urumqi 830000, China. AD - Engineering and Technology Research Center for Veterinary Biological Products, Xinjiang Production and Construction Corps, Urumqi 830000, China. FAU - He, Sun AU - He S AD - Tecon Bio-Technology Co., Ltd., Urumqi 830000, China. AD - Engineering and Technology Research Center for Veterinary Biological Products, Xinjiang Production and Construction Corps, Urumqi 830000, China. FAU - Zhu, Yu AU - Zhu Y AD - College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. FAU - Li, Ye AU - Li Y AD - College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. FAU - Lu, Yuxuan AU - Lu Y AD - College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. FAU - Zhang, Zhengliang AU - Zhang Z AD - College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. FAU - Li, Jinglin AU - Li J AD - College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. FAU - Yan, Genqiang AU - Yan G AD - College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. FAU - Ren, Jingjing AU - Ren J AD - College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. FAU - Jiang, Jianjun AU - Jiang J AD - College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. LA - eng GR - 2023A02007-2/Science and Technology Department of Xinjiang Uyghur Autonomous Region/ GR - RCZK202042/Shihezi University/ PT - Case Reports PT - Journal Article DEP - 20261001 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709902 OTO - NOTNLM OT - Escherichia coli OT - O161 serotype OT - calf OT - case report OT - ibeB OT - meningoencephalitis OT - multidrug resistance COIS- Chengwu Hao and Sun He are affiliated with Tecon Bio-Technology Co., Ltd. The company had no role in the design of the study; in the collection, analysis, or interpretation of the data; in the writing of the manuscript; or in the decision to publish the results. The remaining authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:47 PHST- 2026/08/29 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - vetsci13101037 [pii] AID - vetsci-13-01037 [pii] AID - 10.3390/vetsci13101037 [doi] PST - epublish SO - Vet Sci. 2026 Oct 1;13(10):1037. doi: 10.3390/vetsci13101037. PMID- 42944129 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Sep 29 TI - Recombinant B87 and HLJ0504 VP2 Vaccines Protect Chickens Against Infectious Bursal Disease. LID - 10.3390/vetsci13101033 [doi] LID - 1033 AB - Infectious bursal disease virus (IBDV) causes immunosuppression and bursal damage in chickens, and safer recombinant vaccines that provide durable protection are needed. This study compared the antigenicity, immunogenicity, protective efficacy, safety and antibody persistence of unpurified VP2 preparations and purified VP2 virus-like particles (VLPs) derived from the B87 and HLJ0504 IBDV strains. Recombinant VP2 proteins were expressed in Escherichia coli, purified and characterized, formulated with oil adjuvant and evaluated in specific-pathogen-free White Leghorn chickens. Both VP2 proteins retained antigenicity and assembled into relatively uniform VLPs of approximately 25 nm. Purified VLPs induced stronger antibody responses than corresponding unpurified preparations, with 10 microg producing the highest responses. In a virus neutralization assay, HLJ0504 VP2-based preparations induced the highest neutralizing-antibody titers. Following challenge with virulent IBDV BC6/85, 10 microg of HLJ0504 VLPs and unpurified HLJ0504 VP2 at an agar-gel precipitation titer of 1:10 each protected nine of 10 chickens, similar to a commercial inactivated vaccine, and preserved bursal architecture. No systemic or injection-site adverse reactions were detected and selected recombinant vaccines maintained elevated serum-antibody levels for 20 weeks. Thus, HLJ0504 VP2-based vaccines, particularly the VLPs formulation, are promising safe and protective candidates for controlling infectious bursal disease. FAU - Asif, Tayyab Ali AU - Asif TA AUID- ORCID: 0009-0003-5120-3932 AD - International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China. AD - Longhu Laboratory, Zhengzhou 450046, China. FAU - Chen, Pengyu AU - Chen P AD - International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China. AD - Longhu Laboratory, Zhengzhou 450046, China. FAU - Li, Jiahao AU - Li J AD - International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China. AD - Longhu Laboratory, Zhengzhou 450046, China. FAU - Zhang, Jing AU - Zhang J AD - International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China. AD - Longhu Laboratory, Zhengzhou 450046, China. FAU - Ji, Pengchao AU - Ji P AD - International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China. AD - Longhu Laboratory, Zhengzhou 450046, China. FAU - Jiang, Dawei AU - Jiang D AD - International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China. AD - Longhu Laboratory, Zhengzhou 450046, China. LA - eng PT - Journal Article DEP - 20260929 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709485 OTO - NOTNLM OT - Escherichia coli expression OT - IBDV OT - bursa of Fabricius OT - humoral immune response OT - protective immunity OT - vaccine safety OT - virus-like particles COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/29 CRDT- 2026/10/27 10:47 PHST- 2026/08/10 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/09/29 00:00 [pmc-release] AID - vetsci13101033 [pii] AID - vetsci-13-01033 [pii] AID - 10.3390/vetsci13101033 [doi] PST - epublish SO - Vet Sci. 2026 Sep 29;13(10):1033. doi: 10.3390/vetsci13101033. PMID- 42944128 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Sep 29 TI - Deep Learning and Explainable Artificial Intelligence for Infrared-Thermography-Based CMT-Status Classification in Dairy Cows: A Comparative Evaluation in Egyptian Farms. LID - 10.3390/vetsci13101031 [doi] LID - 1031 AB - Early and accurate identification of mastitis-associated inflammation is critical to increasing animal comfort, reducing financial losses, and enhancing milk quality. Although infrared thermography (IRT) has emerged as a viable non-invasive screening technique, the relative efficacy of convolutional neural networks (CNNs) and Vision Transformers (ViTs) for automated CMT-status classification from thermal udder pictures is still unknown. This work carefully analyzed seven ImageNet-pretrained deep learning architectures using 976 thermal udder images (708 healthy and 268 mastitic images) from 488 Holstein cows (354 healthy cows, 708 images; 134 mastitic cows, 268 images), including two Vision Transformer models (ViT-B/16 and Swin-Tiny) and five CNN models (ResNet-50, DenseNet-121, EfficientNet-B0, MobileNetV2, Inception-V3). Before training, pictures were preprocessed using contrast-limited adaptive histogram equalization (CLAHE), scaled to 224 x 224 pixels, and divided using cow-oriented grouping intended to reduce animal-level data leakage (this grouping could not be independently verified against a ground-truth cow roster; see Limitations). Each model was developed from start to finish and evaluated using an independent hold-out test set and five-fold animal-level cross-validation. DenseNet-121 had the greatest results on the hold-out test set, with an accuracy of 82.2%, an AUC of 0.922, a sensitivity of 90.0%, and a specificity of 79.2%. Additionally, it achieved the highest cross-validation performance (mean AUC = 0.914 +/- 0.019). According to statistical analysis, DenseNet-121 was statistically indistinguishable from Inception-V3 under both tests and from ResNet-50 under the more conservative corrected resampled t-test, but significantly outperformed EfficientNet-B0, MobileNetV2, and both Vision Transformers (p < 0.05), while all CNN models outperformed both Vision Transformer models (p < 0.05). Explainable artificial intelligence (XAI) investigation utilizing Grad-CAM corroborated the biological plausibility of the learnt plausibility characteristics by showing that heat patterns in the udder region had a significant impact on model predictions. Grad-CAM analysis showed that heat patterns in the udder region partly drove model predictions, though attention was occasionally influenced by background regions. The best-performing model was tested without retraining on an independent external cohort of 85 cows from a different farm. Although external performance decreased (accuracy = 60.0%; Cohen's kappa = 0.199), consistent with the expected effects of domain shift, the somatic cell count was significantly higher in CMT-positive cows (p < 0.001; AUC = 0.783), supporting the biological significance of the identified thermal abnormalities. Fine-tuned CNNs, led by DenseNet-121, delivered accurate and reproducible thermal CMT-status classification under internal cross-validation, but transportability to an independent farm was limited (external accuracy = 0.600, kappa = 0.199), indicating that domain adaptation and multi-farm validation are needed before broader deployment. These findings provide strong support for the application of CNN-based deep learning in IRT-assisted CMT-status screening while highlighting the necessity for larger multicenter datasets to further evaluate transformer-based approaches. aptation and multi-farm validation are needed before broader deployment. These findings provide strong support for the application of CNN-based deep learning in IRT-assisted mastitis screening while highlighting the necessity for larger multicenter datasets to further evaluate transformer-based approaches. FAU - Elmaria, Alaa T AU - Elmaria AT AD - Department Artificial Intelligence, Faculty of Computers and Artificial Intelligence, Benha University, Benha 19111, Egypt. FAU - Badr, Elsayed AU - Badr E AD - Department of Scientific Computing, Faculty of Computers and Artificial Intelligence, Benha University, Benha 19111, Egypt. AD - Department of Computer Science, Faculty of Computers and Artificial Intelligence, Misr University for Science and Technology (MUST), Giza P.O. Box 77, Egypt. AD - The Egyptian School of Data Science (ESDS), Benha, Egypt. FAU - Sallam, Sobhy M A AU - Sallam SMA AUID- ORCID: 0000-0002-6949-2387 AD - Department of Animal and Poultry Production, College of Agriculture and Food, Qassim University, Buraydah 51452, Saudi Arabia. FAU - Attia, Marwa F A AU - Attia MFA AD - Animal Production Research Institute, Agricultural Research Center, Giza 12618, Egypt. FAU - Swedian, Sara AU - Swedian S AUID- ORCID: 0000-0003-3183-8044 AD - Department Artificial Intelligence, Faculty of Computers and Artificial Intelligence, Benha University, Benha 19111, Egypt. LA - eng PT - Journal Article DEP - 20260929 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709568 OTO - NOTNLM OT - DenseNet OT - Grad-CAM OT - convolutional neural networks OT - dairy cattle OT - deep learning OT - explainable AI OT - infrared thermography OT - mastitis OT - somatic cell count OT - vision transformer COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/29 CRDT- 2026/10/27 10:47 PHST- 2026/08/09 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/09/29 00:00 [pmc-release] AID - vetsci13101031 [pii] AID - vetsci-13-01031 [pii] AID - 10.3390/vetsci13101031 [doi] PST - epublish SO - Vet Sci. 2026 Sep 29;13(10):1031. doi: 10.3390/vetsci13101031. PMID- 42944120 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Sep 28 TI - A Highly Permissive K-C4 Cell Clone for Genotype II African Swine Fever Virus Reveals Passage-Dependent Hemadsorption Changes Associated with EP402R Deletion. LID - 10.3390/vetsci13101024 [doi] LID - 1024 AB - African swine fever virus (ASFV) research commonly relies on primary porcine alveolar macrophages (PAMs), whose limited availability and variability hinder standardized virus isolation and propagation. This study aimed to establish a continuous cell line highly susceptible to genotype II ASFV and characterize phenotypic and genomic changes during serial passage. Thirty COS-1-derived clones were screened after infection with Korea/Pig/PAJU1/2019 using quantitative real-time polymerase chain reaction, immunofluorescence, and hemadsorption assays. Selected clones underwent serial passage, and viruses from passages 5 and 10 in the most susceptible clone were analyzed by whole-genome sequencing. K-C4 supported stable ASFV replication, with a mean viral genome level of 8.80 +/- 0.55 log(10) copies/mL, compared with 7.38 +/- 0.90 log(10) copies/mL in PAMs. Productive infection was confirmed by p72-specific immunofluorescence. The parental and passage 1 viruses retained hemadsorption, whereas the passage 5 virus lacked detectable hemadsorption despite continued replication, and hemadsorption reappeared by passage 10. Whole-genome analysis identified passage-associated deletions of differing extent within EP402R, encoding the hemadsorption-associated CD2-like viral protein (CD2v), which corresponded with the hemadsorption phenotype at each passage. These findings establish K-C4 as a highly permissive platform for genotype II ASFV propagation and characterization while emphasizing the need to monitor phenotypic and genomic stability during serial passage. FAU - Lee, Eun-Gyeong AU - Lee EG AUID- ORCID: 0009-0001-1079-3417 AD - Laboratory for Infectious Disease Prevention, Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Republic of Korea. FAU - Kim, Ju-Hun AU - Kim JH AD - Department of Pharmacology, College of Pharmacy, Chonnam National University, Gwangju 61186, Republic of Korea. FAU - Lim, Sarah AU - Lim S AD - Laboratory for Infectious Disease Prevention, Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Republic of Korea. FAU - Moon, Sung-Hyun AU - Moon SH AUID- ORCID: 0000-0003-3137-7532 AD - College of Veterinary Medicine and Bio-Safety Research Institute, Jeonbuk National University, Iksan 54596, Republic of Korea. FAU - Gu, Hyunji AU - Gu H AUID- ORCID: 0000-0001-7366-0956 AD - Laboratory for Infectious Disease Prevention, Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Republic of Korea. FAU - Park, Jun Young AU - Park JY AUID- ORCID: 0000-0002-9793-1623 AD - Laboratory for Infectious Disease Prevention, Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Republic of Korea. FAU - Kim, Dae-Min AU - Kim DM AUID- ORCID: 0009-0001-8575-4164 AD - Laboratory for Infectious Disease Prevention, Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Republic of Korea. FAU - Lee, Gun-Hee AU - Lee GH AUID- ORCID: 0000-0003-2320-6514 AD - Laboratory for Infectious Disease Prevention, Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Republic of Korea. FAU - Cho, Ho-Seong AU - Cho HS AUID- ORCID: 0000-0001-7443-167X AD - College of Veterinary Medicine and Bio-Safety Research Institute, Jeonbuk National University, Iksan 54596, Republic of Korea. FAU - Kim, Kyeong-Man AU - Kim KM AD - Department of Pharmacology, College of Pharmacy, Chonnam National University, Gwangju 61186, Republic of Korea. FAU - Tark, Dongseob AU - Tark D AUID- ORCID: 0000-0001-7499-4253 AD - Laboratory for Infectious Disease Prevention, Korea Zoonosis Research Institute, Jeonbuk National University, Iksan 54531, Republic of Korea. LA - eng GR - 2024_JEA1/Jeonbuk Technopark/ PT - Journal Article DEP - 20260928 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709355 OTO - NOTNLM OT - African swine fever virus OT - COS-1 cells OT - EP402R OT - cell cloning OT - cell culture adaptation OT - genotype II OT - hemadsorption COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/28 CRDT- 2026/10/27 10:47 PHST- 2026/08/10 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - vetsci13101024 [pii] AID - vetsci-13-01024 [pii] AID - 10.3390/vetsci13101024 [doi] PST - epublish SO - Vet Sci. 2026 Sep 28;13(10):1024. doi: 10.3390/vetsci13101024. PMID- 42944114 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Sep 26 TI - Integrating Host Transcriptomics and Metagenomics in the Periparturient Dairy Cow: From Molecular Signatures to Host-Microbiome Interfaces. LID - 10.3390/vetsci13101020 [doi] LID - 1020 AB - The periparturient or transition period-roughly three weeks before to three weeks after calving-compresses the most severe metabolic, endocrine and immunological demands of the production cycle into a few weeks and accounts for most clinical disease and involuntary culling in dairy herds. Conventional surveillance relies on downstream indicators-non-esterified fatty acids, beta-hydroxybutyrate, calcium and acute-phase proteins-that document failed adaptation without resolving its regulatory origins. Two omics platforms now close that gap. Transcriptomics shows that the liver, adipose tissue, mammary gland, endometrium, rumen epithelium and circulating leukocytes each execute a distinct, temporally ordered program organized around lipid mobilization, gluconeogenesis, endoplasmic reticulum stress, redox defense and inflammatory resolution, layered over a regulatory tier of non-coding RNAs. Metagenomics shows in parallel that rumen, hindgut, uterine, vaginal, teat-apex and colostrum communities are restructured by parturition and by the dietary shift into lactation, with reproducible dysbiotic signatures accompanying ketosis, fatty liver, metritis, endometritis and intramammary infection. Critically, the two layers are mechanistically coupled: microbial short-chain fatty acids, secondary bile acids, purine derivatives and amino acids reprogram hepatic and immune cell transcription, while host lipolysis, inflammation and mineral demand reshape community structure. This review synthesizes both groups of literature within a single framework, formalizes five host-microbiome interfaces supported by paired measurements and identifies the biomarker, nutritional and genetic-selection opportunities that emerge only when host and microbial evidence are read together. FAU - Ma, Qingshan AU - Ma Q AD - College of Agriculture and Biology, Liaocheng University, Liaocheng 252000, China. FAU - Tharwat, Mohamed AU - Tharwat M AUID- ORCID: 0000-0002-3796-9590 AD - Department of Clinical Sciences, College of Veterinary Medicine, Qassim University, P.O. Box 6622, Buraidah 51452, Saudi Arabia. FAU - Ullah, Abd AU - Ullah A AUID- ORCID: 0009-0004-6839-7849 AD - College of Agriculture and Biology, Liaocheng University, Liaocheng 252000, China. FAU - Liu, Junjie AU - Liu J AD - College of Agriculture and Biology, Liaocheng University, Liaocheng 252000, China. FAU - Alkheraif, Abdulrahman A AU - Alkheraif AA AD - Department of Pathology and Laboratory Diagnosis, College of Veterinary Medicine, Qassim University, P.O. Box 6622, Buraidah 51452, Saudi Arabia. FAU - Khan, Muhammad Zahoor AU - Khan MZ AUID- ORCID: 0000-0001-5890-2996 AD - College of Agriculture and Biology, Liaocheng University, Liaocheng 252000, China. LA - eng GR - QU-APC-2026/Qassim University/ PT - Journal Article PT - Review DEP - 20260926 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709425 OTO - NOTNLM OT - immunometabolism OT - ketosis OT - metagenome OT - metritis OT - multi-omics integration OT - negative energy balance OT - rumen and hindgut microbiome OT - transcriptomics OT - transition cow COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/26 CRDT- 2026/10/27 10:47 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/09/26 00:00 [pmc-release] AID - vetsci13101020 [pii] AID - vetsci-13-01020 [pii] AID - 10.3390/vetsci13101020 [doi] PST - epublish SO - Vet Sci. 2026 Sep 26;13(10):1020. doi: 10.3390/vetsci13101020. PMID- 42944112 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Sep 26 TI - Descriptive, Exploratory Analysis of Microbial Community Composition Across Stages of Canine Periodontal Disease Revealed by 16S rRNA Sequencing in Dogs. LID - 10.3390/vetsci13101016 [doi] LID - 1016 AB - Periodontal disease (PD) is one of the most prevalent oral conditions in dogs, yet the microbial dynamics underlying its progression remain incompletely understood. This exploratory study aimed to describe the canine oral microbiome across four clinical stages using 16S rRNA gene sequencing. Dogs were categorized as Healthy (Stage 0), Gingivitis (Stage 1), Early Periodontitis (Stage 2), and Moderate-Advanced Periodontitis (Stages 3-4) according to American Veterinary Dental College (AVDC) criteria. Pooled subgingival plaque samples from the groups were analyzed for microbial diversity, taxonomic composition, and ecological patterns. Alpha diversity differed among the four pooled profiles, with the highest observed richness in the Early Periodontitis (Stage 2) pool and the lowest in the Moderate-Advanced Periodontitis (Stages 3-4) pool. Beta diversity analyses showed separation among the pooled profiles, particularly between the healthy and severe groups. Taxonomic profiling identified higher relative abundances of sequences assigned to Porphyromonas gingivalis and Treponema denticola in the more advanced disease stage pools, while Fusobacterium nucleatum reached its highest relative abundance in the Early Periodontitis (Stage 2) pool. Visualization of abundance profiles across the four pooled libraries revealed concordant trends among selected treponeme taxa and Fusobacterium nucleatum, as well as inverse trends for other taxa. These findings provide a descriptive, group-level characterization of microbial community differences across AVDC-defined stages of canine periodontal disease based on pooled samples and generate hypotheses for future individual-level studies. FAU - Pizauro, Lucas Jose Luduverio AU - Pizauro LJL AUID- ORCID: 0000-0002-1071-8740 AD - Department of Agricultural and Environmental Sciences, Santa Cruz State University, Ilheus 45662-900, Brazil. FAU - Gualberto, Nina Gabriela Silva AU - Gualberto NGS AD - Graduate Program in Animal Science, Department of Agricultural and Environmental Sciences, Santa Cruz State University, Ilheus 45662-900, Brazil. FAU - Santos, Katharine Costa AU - Santos KC AD - Graduate Program in Animal Science, Department of Agricultural and Environmental Sciences, Santa Cruz State University, Ilheus 45662-900, Brazil. FAU - Pirovani, Carlos Priminho AU - Pirovani CP AUID- ORCID: 0000-0002-6176-4069 AD - Department of Biological Sciences, Santa Cruz State University, Ilheus 45662-900, Brazil. FAU - Santana, Juliano Oliveira AU - Santana JO AD - Graduate Program in Genetics and Molecular Biology, Santa Cruz State University, Ilheus 45662-900, Brazil. FAU - Castro, Luiza Montenegro Cintra AU - Castro LMC AD - Independent Researcher, Ilheus 45662-900, Brazil. FAU - Dias, Ricardo Pedro Moreira AU - Dias RPM AD - Biosystems & Integrative Sciences Institute, Faculty of Sciences, University of Lisbon, 1749-016 Lisbon, Portugal. AD - CE3C-Centre for Ecology, Evolution and Environmental Changes, Institute for Global Change and Sustainability, 1749-016 Lisbon, Portugal. FAU - Nunes, Monica Susana Claudino AU - Nunes MSC AUID- ORCID: 0000-0001-5034-5494 AD - CE3C-Centre for Ecology, Evolution and Environmental Changes, Institute for Global Change and Sustainability, 1749-016 Lisbon, Portugal. FAU - Carlos, Renata Santiago Alberto AU - Carlos RSA AUID- ORCID: 0000-0001-7879-1824 AD - Department of Agricultural and Environmental Sciences, Santa Cruz State University, Ilheus 45662-900, Brazil. LA - eng PT - Journal Article DEP - 20260926 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709691 OTO - NOTNLM OT - Fusobacterium sp. OT - Porphyromonas sp. OT - Treponema sp. OT - dysbiosis OT - microbial diversity OT - oral microbiota COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/26 CRDT- 2026/10/27 10:47 PHST- 2026/08/03 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/09/26 00:00 [pmc-release] AID - vetsci13101016 [pii] AID - vetsci-13-01016 [pii] AID - 10.3390/vetsci13101016 [doi] PST - epublish SO - Vet Sci. 2026 Sep 26;13(10):1016. doi: 10.3390/vetsci13101016. PMID- 42944105 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Sep 24 TI - Dairy Cattle Feed Upcycling, Adaptogenic Nutraceuticals and Precision Reproduction: Advanced CASA Sperm Kinematic Profiling and Sexual Drive in Rams. LID - 10.3390/vetsci13101009 [doi] LID - 1009 AB - This study capitalizes on computer-assisted semen analysis (CASA) to evaluate a precision nutritional strategy integrating agro-industrial feed upcycling and functional phytogenic supplementation in rams. Black Belly rams (n = 15) sustained on an upcycled basal diet derived from dairy-cow feed rejections were assigned to a non-supplemented control group (CONT) or supplemented with Withania somnifera (WITH) or Lepidium meyenii (LEPI) at 400 mg kg(-1) LW d(-1). The 64-day trial was conducted from May to July in arid northern Mexico. Sexual drive, seminal quality, and high-resolution sperm kinematics were evaluated using CASA. Body and testicular measurements, ejaculate volume, and most categorical motility traits remained comparable among treatments (p > 0.05). However, phytogenic supplementation enhanced selected seminal and sexual-drive variables (p < 0.05). Crucially, longitudinal treatment x time interactions (p < 0.05) showed that while the CONT group experienced a seasonal decline in local mobility (LOCMOB), it increased from 1.47% to 2.01% in the WITH group, representing a 37% relative increase over time. Moreover, both WITH and LEPI increased LIN and STR by the end of the experimental period (p < 0.05), demonstrating more linear and efficient sperm trajectories. These findings highlight the productive value of upcycled dairy-cow feed rejections and demonstrate that strategic supplementation with W. somnifera or L. meyenii can refine sexual drive, seminal quality, and sperm kinematic efficiency. This integrated bio-circular strategy provides actionable insights for sustainable small-ruminant production under challenging arid summer conditions. FAU - Meza-Herrera, Cesar A AU - Meza-Herrera CA AUID- ORCID: 0000-0003-0289-2009 AD - Graduate Program-Natural Resources and Environment in Arid Lands, Regional Universitary Unit on Arid Lands, Chapingo Autonomous University, Bermejillo 35230, Durango, Mexico. FAU - De Santiago-Miramontes, Angeles AU - De Santiago-Miramontes A AUID- ORCID: 0000-0002-5491-0662 AD - Graduate Program-Agricultural and Livestock Sciences, Laguna Unit, Antonio Narro Agrarian Autonomous University, Torreon 27054, Coahuila, Mexico. FAU - Rodriguez-Sanchez, Andres J AU - Rodriguez-Sanchez AJ AD - Graduate Program-Agricultural and Livestock Sciences, Laguna Unit, Antonio Narro Agrarian Autonomous University, Torreon 27054, Coahuila, Mexico. FAU - Ramirez-Uranga, Karla Q AU - Ramirez-Uranga KQ AUID- ORCID: 0000-0003-1139-6883 AD - Graduate Program-Natural Resources and Environment in Arid Lands, Regional Universitary Unit on Arid Lands, Chapingo Autonomous University, Bermejillo 35230, Durango, Mexico. AD - Graduate Program-Agricultural and Livestock Sciences, Laguna Unit, Antonio Narro Agrarian Autonomous University, Torreon 27054, Coahuila, Mexico. FAU - Santos-Silva, Raquel AU - Santos-Silva R AUID- ORCID: 0009-0001-5874-3881 AD - Graduate Program-Natural Resources and Environment in Arid Lands, Regional Universitary Unit on Arid Lands, Chapingo Autonomous University, Bermejillo 35230, Durango, Mexico. FAU - Macias-Cruz, Ulises AU - Macias-Cruz U AD - Institute of Agricultural Sciences, Autonomous University of Baja California, Mexicali 21705, Baja California, Mexico. FAU - Navarrete-Molina, Cayetano AU - Navarrete-Molina C AUID- ORCID: 0000-0001-7867-2745 AD - Graduate Program-Natural Resources and Environment in Arid Lands, Regional Universitary Unit on Arid Lands, Chapingo Autonomous University, Bermejillo 35230, Durango, Mexico. FAU - Vargas-Garcia, Denia L AU - Vargas-Garcia DL AD - Graduate Program-Agricultural and Livestock Sciences, Laguna Unit, Antonio Narro Agrarian Autonomous University, Torreon 27054, Coahuila, Mexico. FAU - Rodriguez-Gonzalez, Mayela AU - Rodriguez-Gonzalez M AD - Division of Health, Biological and Environmental Sciences, Open and Distance University of Mexico, Ciudad de Mexico 03330, Mexico. FAU - Luna-Zapien, Eden A AU - Luna-Zapien EA AD - Faculty of Chemical Sciences, Juarez University of the State of Durango, Gomez Palacio 35010, Durango, Mexico. FAU - Sarinana-Navarrete, Maria A AU - Sarinana-Navarrete MA AUID- ORCID: 0000-0002-0936-1692 AD - Department of Sustainable and Protected Agriculture, Technological University of Rodeo, Rodeo 35760, Durango, Mexico. LA - eng PT - Journal Article DEP - 20260924 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709658 OTO - NOTNLM OT - Lepidium meyenii OT - Withania somnifera OT - circular economy OT - semen-sperm quality OT - sexual drive OT - sperm kinematics OT - trajectory linearity OT - upcycled dairy orts COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:47 PHST- 2026/08/28 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - vetsci13101009 [pii] AID - vetsci-13-01009 [pii] AID - 10.3390/vetsci13101009 [doi] PST - epublish SO - Vet Sci. 2026 Sep 24;13(10):1009. doi: 10.3390/vetsci13101009. PMID- 42944104 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Sep 24 TI - Dose-Related Associations of a Palm Kernel Meal-Derived Mannan-Oligosaccharide-Containing Hydrolysate with Selected Immune and Ruminal Fermentation Measures in Growing Goats. LID - 10.3390/vetsci13101008 [doi] LID - 1008 AB - Mannan-oligosaccharides (MOS) may influence microbial and immune-related processes, but evidence concerning palm kernel meal (PKM)-derived MOS-containing products in goats remains limited. The experimental product was a chemically heterogeneous PKM-derived hydrolysate in which M2-M6 oligosaccharides were identified by HPLC-RID; residual sugars and other soluble constituents were also present. Twenty growing male goats received 0, 2.5, 5.0, or 10.0 g hydrolysate/goat/day (n = 5 per treatment) for 90 days. Feed intake, growth, hematological and serum biochemical indices, circulating immune indicators, peripheral blood leukocyte gene expression, and selected ruminal measures were evaluated. Immune and gene-expression outcomes were measured in prefeeding blood collected on Day 90. Ruminal pH and acetate, propionate, and butyrate were measured in a single rumen-fluid sample collected 3 h after feeding on Day 90. Serum IgA showed a positive linear association with supplementation level, whereas IL-1beta and IL-6 showed negative associations; these remained significant after false-discovery-rate correction (q = 0.036, 0.012, and 0.012, respectively). Selected immune-related transcripts also differed among treatments, but these endpoint patterns do not establish coordinated immunological regulation or a specific mechanism. Propionate and butyrate showed nominal positive linear associations, whereas the acetate-to-propionate ratio showed a nominal negative association (raw p = 0.028, 0.021, and 0.018, respectively); none remained significant after correction (q = 0.056 for each). Ruminal pH, the sum of the three measured VFA concentrations, feed intake, and growth did not differ detectably. No overt clinical abnormalities requiring veterinary intervention were observed, but the study was not powered to establish safety. These preliminary between-group endpoint associations concern the complete heterogeneous hydrolysate and cannot be attributed specifically to M2-M6 oligosaccharides. Larger, adequately powered studies with pretreatment and repeated measurements are required. FAU - Roekngam, Natthrit AU - Roekngam N AUID- ORCID: 0009-0000-2555-6919 AD - Faculty of Veterinary Science, Rajamangala University of Technology Srivijaya, Nakhon Si Thammarat 80240, Thailand. FAU - Chuchai, Supinya AU - Chuchai S AD - Faculty of Veterinary Science, Rajamangala University of Technology Srivijaya, Nakhon Si Thammarat 80240, Thailand. FAU - Thongmanee, Porntip AU - Thongmanee P AD - Faculty of Veterinary Science, Rajamangala University of Technology Srivijaya, Nakhon Si Thammarat 80240, Thailand. FAU - Thongtem, Niramol AU - Thongtem N AD - Faculty of Veterinary Science, Rajamangala University of Technology Srivijaya, Nakhon Si Thammarat 80240, Thailand. FAU - Chaisit, Suwichak AU - Chaisit S AUID- ORCID: 0000-0003-3233-0111 AD - Department of Chemical Engineering and Pharmaceutical Chemistry, School of Engineering and Technology, Walailak University, Nakhon Si Thammarat 80160, Thailand. AD - Biomass and Oil Palm Center of Excellence, Walailak University, Nakhon Si Thammarat 80160, Thailand. FAU - Bohman, Wiruntita AU - Bohman W AUID- ORCID: 0009-0005-9381-0743 AD - Faculty of Veterinary Science, Rajamangala University of Technology Srivijaya, Nakhon Si Thammarat 80240, Thailand. FAU - Khongthong, Sunisa AU - Khongthong S AD - Faculty of Veterinary Science, Rajamangala University of Technology Srivijaya, Nakhon Si Thammarat 80240, Thailand. LA - eng GR - 203994/Rajamangala University of Technology Srivijaya/ PT - Journal Article DEP - 20260924 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709411 OTO - NOTNLM OT - agro-industrial by-product OT - circular bioeconomy OT - cytokines OT - immunoglobulin A OT - inflammatory signaling OT - prebiotic OT - small ruminants OT - volatile fatty acids COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:47 PHST- 2026/08/19 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - vetsci13101008 [pii] AID - vetsci-13-01008 [pii] AID - 10.3390/vetsci13101008 [doi] PST - epublish SO - Vet Sci. 2026 Sep 24;13(10):1008. doi: 10.3390/vetsci13101008. PMID- 42944103 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Sep 24 TI - Algal Biomass and Algal-Derived Preparations in Livestock Nutrition: Gut Microbiota Modulation, Host Molecular Pathways, and Bioprocess Innovations. LID - 10.3390/vetsci13101007 [doi] LID - 1007 AB - Algal biomass and algal-derived preparations have attracted increasing interest in livestock nutrition because of their potential to modulate the gut microbiota, which plays a crucial role in animal health, immunity, and growth performance. This review focuses primarily on microalgae and cyanobacterial preparations, while also considering selected macroalgae relevant to livestock feeding, without restricting its scope to marine sources. In this narrative review, we summarize recent advances in the physicochemical properties and biological activities of key algal components, such as polysaccharides, polyunsaturated fatty acids, and carotenoids. We assess evidence for changes in potentially beneficial bacteria, including Lactobacillus and Bifidobacterium, and for inhibitory effects against selected pathogenic bacteria, including Salmonella and pathogenic strains of Escherichia coli. We also examine proposed links between these compounds, microbial short-chain fatty acid and bile acid metabolism, and host immune and growth responses. This review discusses practical applications of algal biomass and algal-derived preparations in livestock production, with a focus on inclusion levels and safety assessments. Effects are evaluated in relation to the source organism, preparation, and animal model, rather than assumed to be shared across algal groups. Current challenges and future research directions are also identified to facilitate the efficient utilization of algal resources in sustainable animal husbandry. This review identifies the evidence supporting specific feeding applications and distinguishes observed animal responses from proposed mechanisms. FAU - Lu, Fei AU - Lu F AD - Nantong Marine Center, Ministry of Natural Resources, Nantong 226000, China. FAU - Liu, Ziming AU - Liu Z AD - Nantong Natural Resources Affairs Service Center, Nantong 226000, China. FAU - Cao, Bing AU - Cao B AD - Nantong Marine Center, Ministry of Natural Resources, Nantong 226000, China. FAU - Wu, Chengyang AU - Wu C AD - Nantong Marine Center, Ministry of Natural Resources, Nantong 226000, China. FAU - He, Peidong AU - He P AD - Nantong Marine Center, Ministry of Natural Resources, Nantong 226000, China. FAU - Li, Lihua AU - Li L AD - Nantong Marine Center, Ministry of Natural Resources, Nantong 226000, China. FAU - Chen, Zhongwei AU - Chen Z AD - Nantong Marine Center, Ministry of Natural Resources, Nantong 226000, China. FAU - Ge, Yuehao AU - Ge Y AD - Nantong Marine Center, Ministry of Natural Resources, Nantong 226000, China. LA - eng PT - Journal Article PT - Review DEP - 20260924 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709582 OTO - NOTNLM OT - algae OT - bioactive compounds OT - cyanobacteria OT - gut microbiota OT - host signaling pathways OT - livestock nutrition OT - macroalgae OT - microalgae COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:47 PHST- 2026/08/04 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - vetsci13101007 [pii] AID - vetsci-13-01007 [pii] AID - 10.3390/vetsci13101007 [doi] PST - epublish SO - Vet Sci. 2026 Sep 24;13(10):1007. doi: 10.3390/vetsci13101007. PMID- 42944101 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Sep 23 TI - Pet Insurance and Veterinary Treatment Decisions: A Game-Theoretic Model of the Owner-Veterinarian Relationship in Turkiye. LID - 10.3390/vetsci13101005 [doi] LID - 1005 AB - The economic benefits of pet insurance are generally examined through survey-based empirical studies, while a game-theoretic explanation of how insurance changes treatment decisions remains limited. This study models the clinical decision process between a pet owner and a veterinarian as a static, two-player game within expected utility theory, using an exponential (constant absolute risk aversion, CARA) utility function. The veterinarian's strategy is Basic, Comprehensive Treatment, the owner's is Accept, Reject; severity is a two-state random variable (Mild/Severe). Parameters are calibrated using Turkish market data from the author's previous study. Findings show that in the uninsured regime, a unique Nash equilibrium (Basic Treatment, Reject) endogenously produces economic treatment-refusal behavior; insurance, by altering the payoff structure, makes available a second, Pareto-superior equilibrium (Comprehensive Treatment, Accept). Sensitivity analyses show comprehensive treatment becomes relatively more attractive as risk aversion increases, but once the co-payment rate exceeds a threshold (~46.3%), insurance loses this effect entirely. Premium level does not affect equilibrium selection, being a sunk cost independent of the treatment decision. These findings indicate that the co-payment rate is a more critical policy variable than premium level in insurance design, and offer a game-theoretic explanation for how pet insurance may affect the veterinarian-owner treatment decision. FAU - Gencgonul, Betul Zehra AU - Gencgonul BZ AUID- ORCID: 0000-0002-9964-4521 AD - Department of Actuarial Sciences, Hacettepe University, 06800 Ankara, Turkiye. LA - eng PT - Journal Article DEP - 20260923 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709341 OTO - NOTNLM OT - Nash equilibrium OT - game theory OT - pet insurance OT - treatment-acceptance threshold OT - utility function OT - veterinarian-owner relationship COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/23 CRDT- 2026/10/27 10:47 PHST- 2026/08/27 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - vetsci13101005 [pii] AID - vetsci-13-01005 [pii] AID - 10.3390/vetsci13101005 [doi] PST - epublish SO - Vet Sci. 2026 Sep 23;13(10):1005. doi: 10.3390/vetsci13101005. PMID- 42944099 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Sep 23 TI - Maternal Metabolic Profiles, Their Relationships with Progesterone and Pregnancy-Associated Glycoprotein (PAG), and Biomarker Potential During Early Pregnancy in Lactating Ewes. LID - 10.3390/vetsci13101003 [doi] LID - 1003 AB - This study aimed to characterize the longitudinal maternal biochemical phenotype associated with early pregnancy, determine its relationships with progesterone (P(4)) and pregnancy-associated glycoproteins (PAGs), and conduct an exploratory evaluation of the prognostic and discriminatory potential of selected variables. Following synchronized breeding outside the breeding season, serum samples were collected from lactating ewes with confirmed singleton pregnancies (n = 22) and non-pregnant ewes (n = 22) at six time points from mating to D50. Pregnant ewes showed lower total protein and globulin, higher albumin-to-globulin ratio (A/G), and higher lactate dehydrogenase (LDH) from D7 to D35. Total oxidant status (TOS) was lower at mating and D14 in ewes that subsequently became pregnant. In pooled analyses, the P(4)-PAG correlation increased from D21 (r = 0.353) to D50 (r = 0.844), whereas most biochemical associations were attenuated in pregnancy-only analyses, suggesting that some pooled relationships were partly influenced by pregnancy-status separation. TOS showed the highest apparent prognostic performance at mating (AUC = 0.939). On D7, P(4) (AUC = 0.948) and LDH (AUC = 0.915) showed the highest apparent individual discriminatory performance. By D21, P(4) provided complete in-sample discrimination (AUC = 1.000), while A/G was the highest apparent performance among non-hormonal variables (AUC = 0.916). Exploratory multivariable models indicated the greatest apparent potential for biochemical-endocrine complementarity during D7-D14. These findings support a dynamic maternal biochemical phenotype and identify routinely measurable candidate variables that may complement luteal and conceptus-placental indicators, although these performance estimates are hypothesis-generating and require external validation. FAU - Uyanik, Gokhan AU - Uyanik G AD - Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, 31060 Hatay, Turkiye. FAU - Gozer, Ahmet AU - Gozer A AUID- ORCID: 0000-0001-8658-5916 AD - Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, 31060 Hatay, Turkiye. FAU - Abay, Murat AU - Abay M AUID- ORCID: 0000-0003-2457-1919 AD - Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Erciyes University, 38280 Kayseri, Turkiye. FAU - Kaya, Ufuk AU - Kaya U AD - Department of Biostatistics, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, 31060 Hatay, Turkiye. FAU - Gokcek, Ishak AU - Gokcek I AD - Department of Physiology, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, 31060 Hatay, Turkiye. FAU - Sertkol, Ramazan AU - Sertkol R AD - Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, 31060 Hatay, Turkiye. LA - eng PT - Journal Article DEP - 20260923 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709780 OTO - NOTNLM OT - ewes OT - metabolic profile OT - oxidative stress OT - pregnancy OT - pregnancy-associated glycoproteins OT - progesterone COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/23 CRDT- 2026/10/27 10:47 PHST- 2026/08/27 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - vetsci13101003 [pii] AID - vetsci-13-01003 [pii] AID - 10.3390/vetsci13101003 [doi] PST - epublish SO - Vet Sci. 2026 Sep 23;13(10):1003. doi: 10.3390/vetsci13101003. PMID- 42944098 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Sep 23 TI - Mechanistic Investigation of Cryptotanshinone and Curcumin Combination in Attenuating BVDV-1-Induced Injury in Bovine Ovarian Granulosa Cells. LID - 10.3390/vetsci13101002 [doi] LID - 1002 AB - Bovine viral diarrhea virus (BVDV) is a widespread pathogen causing significant economic losses in cattle. There is an urgent need for drugs to treat BVDV-induced reproductive dysfunction. This study investigated the mechanisms and targets of the cryptotanshinone-curcumin (CRY-CUR) combination against BVDV-1. Overlapping targets of CRY, CUR, and BVDV were screened using TCMSP, ChEMBL, and GeneCards databases. A Venn diagram was generated, followed by GO and KEGG enrichment analyses via DAVID. A PPI network was constructed using Cytoscape, and molecular docking was performed with AutoDock. Anti-BVDV-1 activity was verified in bovine ovarian granulosa cells (BOGCs) using RT-qPCR and immunofluorescence. A total of 108 overlapping targets were identified, with four core targets (STAT3, TNF, BCL-2, and EGFR) selected. Molecular docking showed strong binding affinities. Enrichment analysis indicated involvement in calcium ion binding, apoptosis, and TNF/NF-kappaB pathways. In vitro, the CRY-CUR combination suppressed BVDV-1 replication and reversed abnormal mRNA expression of the core targets. The CRY-CUR combination may inhibit BVDV-1 replication, potentially through modulation of BVDV-1-related targets such as STAT3, TNF, BCL-2, and EGFR and their associated signaling pathways. FAU - Chen, Xiaoliang AU - Chen X AD - Hebei Key Laboratory of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071000, China. FAU - Zhang, Kang AU - Zhang K AD - Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs of China, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China. FAU - Feng, Haipeng AU - Feng H AD - Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs of China, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China. FAU - Wang, Lei AU - Wang L AD - Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs of China, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China. FAU - Zhang, Jingyan AU - Zhang J AD - Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs of China, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China. FAU - Lu, Xiaorong AU - Lu X AD - Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs of China, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China. FAU - Xu, Guowei AU - Xu G AD - Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs of China, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China. FAU - Liu, Siqi AU - Liu S AD - Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs of China, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China. AD - College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China. FAU - Feng, Xiaowei AU - Feng X AD - Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs of China, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China. FAU - Wang, Junyan AU - Wang J AD - Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs of China, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China. AD - College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China. FAU - Li, Jianxi AU - Li J AD - Hebei Key Laboratory of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071000, China. AD - Traditional Chinese Veterinary Technology Innovation Center of Gansu Province, Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs of China, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of Chinese Academy of Agricultural Sciences, Lanzhou 730050, China. LA - eng GR - CARS-35/China Agriculture Research System/ GR - 24ZDNA001/Major Agricultural Research Project of Gansu Province/ PT - Journal Article DEP - 20260923 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709452 OTO - NOTNLM OT - BOGCs OT - BVDV-1 OT - cryptotanshinone OT - curcumin OT - mechanism of action OT - molecular docking OT - network pharmacology COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/23 CRDT- 2026/10/27 10:47 PHST- 2026/08/17 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - vetsci13101002 [pii] AID - vetsci-13-01002 [pii] AID - 10.3390/vetsci13101002 [doi] PST - epublish SO - Vet Sci. 2026 Sep 23;13(10):1002. doi: 10.3390/vetsci13101002. PMID- 42944095 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Sep 23 TI - Immunohistochemical Expression of Tyrosine Kinase Receptors EGFR and CD117 in Canine Urothelial Carcinoma. LID - 10.3390/vetsci13100999 [doi] LID - 999 AB - Canine urothelial carcinoma is the most common malignant neoplasm of the lower urinary tract in dogs and is characterized by aggressive biological behavior, high invasiveness, and metastatic potential. Despite advances in our understanding of this neoplasm, few molecular biomarkers with diagnostic, prognostic, and therapeutic value have been identified. In this context, the present study aimed to evaluate the immunohistochemical expression of the tyrosine kinase receptors EGFR (Epidermal Growth Factor Receptor) and CD117 (c-KIT) in canine urothelial carcinomas. EGFR and CD117 were positive in the majority of cases; both were individually associated with a high histologic grade. In multivariate analysis, a high histological grade was independently associated with EGFR, whereas CD117 was not. In a separate model, EGFR/CD117 co-expression was also independently associated with high histological grade. EGFR and CD117 expression were themselves significantly associated, with 95.0% of EGFR-positive tumors also CD117-positive, a pattern that warrants further investigation into a potential interplay between these pathways during urothelial carcinogenesis. To our knowledge, this is the first study to simultaneously assess EGFR and CD117 in canine urothelial carcinoma; these findings support further investigation into their potential as combined therapeutic targets and suggest that the canine model may offer valuable insights for human precision oncology. FAU - Files, Rita AU - Files R AUID- ORCID: 0009-0009-6211-850X AD - Department of Veterinary Sciences, University of Tras-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal. FAU - Nunes, Catarina AU - Nunes C AD - Department of Biology and Environment, School of Life and Environmental Sciences, University of Tras-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal. FAU - Cardoso, Maria AU - Cardoso M AD - Department of Biology and Environment, School of Life and Environmental Sciences, University of Tras-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal. FAU - Silva, Maria AU - Silva M AD - Department of Veterinary Sciences, University of Tras-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal. FAU - Delgado, Leonor AU - Delgado L AUID- ORCID: 0000-0002-4405-0240 AD - UNIPRO-Oral Pathology and Rehabilitation Research Unit, University Institute of Health Sciences-CESPU (IUCS-CESPU), 4585-116 Gandra, Portugal. AD - Department of Pathology, INNO Specialized Veterinary Services, 4710-503 Braga, Portugal. FAU - Prada, Justina AU - Prada J AUID- ORCID: 0000-0002-3902-5374 AD - Department of Veterinary Sciences, University of Tras-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal. AD - Animal and Veterinary Research Centre (CECAV), Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), University of Tras-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal. FAU - Pires, Isabel AU - Pires I AUID- ORCID: 0000-0001-6330-4560 AD - Department of Veterinary Sciences, University of Tras-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal. AD - Animal and Veterinary Research Centre (CECAV), Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), University of Tras-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal. LA - eng GR - AICanceRinfinityHub project (NORTE2030-FE-DER-02687800), co-financed by the European Regional Development Fund (ERDF) under the Northern Regional Program 2021-2027 [NORTE2030]./AICanceRinfinityHub project (NORTE2030-FE-DER-02687800), co-financed by the European Regional Development Fund (ERDF) under the Northern Regional Program 2021-2027 [NORTE2030]./ PT - Journal Article DEP - 20260923 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709803 OTO - NOTNLM OT - EGFR OT - bladder cancer OT - c-KIT OT - dogs OT - immunohistochemistry OT - translational medicine OT - tumor aggressiveness COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/23 CRDT- 2026/10/27 10:47 PHST- 2026/08/08 00:00 [received] PHST- 2026/09/09 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - vetsci13100999 [pii] AID - vetsci-13-00999 [pii] AID - 10.3390/vetsci13100999 [doi] PST - epublish SO - Vet Sci. 2026 Sep 23;13(10):999. doi: 10.3390/vetsci13100999. PMID- 42944094 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 13 IP - 10 DP - 2026 Sep 23 TI - Serum IgE, IL-4 and IL-13 Concentrations as Th2-Associated Biomarkers in Canine Giardiasis. LID - 10.3390/vetsci13100998 [doi] LID - 998 AB - Giardia duodenalis is a common intestinal protozoan parasite in dogs, but the systemic immune response associated with canine giardiasis remains incompletely characterized. This study aimed to evaluate serum concentrations of immunoglobulin E (IgE), interleukin-4 (IL-4), and interleukin-13 (IL-13) as Th2-associated immune markers in naturally infected dogs. Thirty client-owned dogs with confirmed giardiasis and 10 clinically healthy control dogs were included in the study. Serum IgE, IL-4, and IL-13 concentrations were measured using canine-specific enzyme-linked immunosorbent assays. Between-group comparisons were performed using two-sided Mann-Whitney U tests, with Holm adjustment for multiple comparisons, and effect estimates were calculated using the Hodges-Lehmann method. Spearman's rank correlation was used to assess associations among biomarkers within each group. Serum IL-4 concentrations were significantly higher in dogs with giardiasis than in control dogs (median 38.12 [IQR 32.55-57.97] vs. 23.65 [18.86-27.90] pg/mL; Mann-Whitney U = 238.5, Holm-adjusted p < 0.001), with a Hodges-Lehmann estimated location shift of 16.31 pg/mL (95% CI: 9.80-27.47). Serum IgE concentrations were higher in dogs with giardiasis but did not remain statistically significant after Holm adjustment (1.32 [1.15-1.62] vs. 1.16 [1.02-1.33] microg/mL; Holm-adjusted p = 0.125), while IL-13 concentrations did not differ between groups (78.23 [53.05-103.42] vs. 79.05 [65.44-103.68] ng/L; Holm-adjusted p = 0.988). Within the giardiasis group, IgE, IL-4, and IL-13 concentrations were significantly and positively correlated with one another (rho = 0.644-0.690, p < 0.001). In control dogs, only IL-4 and IL-13 were significantly correlated (rho = 0.636, p = 0.048). These findings provide evidence consistent with altered Th2-associated immune activity in canine giardiasis, characterized by a significant elevation of serum IL-4 and coordinated variation among Th2-associated biomarkers. The non-significant findings for serum IgE and IL-13 may reflect a selective pattern of systemic Th2-associated biomarker expression, although limited statistical power prevents exclusion of smaller between-group differences. Further studies incorporating intestinal mucosal measurements and longitudinal assessment are warranted to clarify the relationship between systemic and local immune responses in canine giardiasis. FAU - Durgut, Murat Kaan AU - Durgut MK AUID- ORCID: 0000-0002-4582-8536 AD - Department of Internal Medicine, Faculty of Veterinary Medicine, Selcuk University, Konya 42000, Turkey. FAU - Ok, Mahmut AU - Ok M AUID- ORCID: 0000-0002-8210-6735 AD - Department of Internal Medicine, Faculty of Veterinary Medicine, Selcuk University, Konya 42000, Turkey. FAU - Iyigun, Suleyman Serhat AU - Iyigun SS AUID- ORCID: 0000-0002-3270-1931 AD - Department of Internal Medicine, Faculty of Veterinary Medicine, Selcuk University, Konya 42000, Turkey. FAU - Akkus, Hazal AU - Akkus H AUID- ORCID: 0009-0002-7549-4482 AD - Department of Internal Medicine, Faculty of Veterinary Medicine, Selcuk University, Konya 42000, Turkey. FAU - Sivrioglu, Gamze AU - Sivrioglu G AUID- ORCID: 0009-0000-6923-9819 AD - Department of Internal Medicine, Faculty of Veterinary Medicine, Selcuk University, Konya 42000, Turkey. LA - eng PT - Journal Article DEP - 20260923 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC13709580 OTO - NOTNLM OT - Th2 immune response OT - canine giardiasis OT - immunoglobulin E OT - interleukin-13 OT - interleukin-4 COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/23 CRDT- 2026/10/27 10:47 PHST- 2026/08/27 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - vetsci13100998 [pii] AID - vetsci-13-00998 [pii] AID - 10.3390/vetsci13100998 [doi] PST - epublish SO - Vet Sci. 2026 Sep 23;13(10):998. doi: 10.3390/vetsci13100998. PMID- 42944079 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Oct 19 TI - Vascular Programs in Emerging and Re-Emerging RNA Virus Infections: Comparative Lessons from SARS-CoV-2, Flaviviruses, and Hantaviruses. LID - 10.3390/v18101160 [doi] LID - 1160 AB - Emerging and re-emerging RNA viruses frequently present with clinical phenotypes that extend beyond classical organ tropism and include vascular leakage, platelet dysregulation, coagulation imbalance, microvascular injury, and thromboinflammation. SARS-CoV-2, flaviviruses, and hantaviruses provide three complementary models of virus-induced vascular disease. This narrative review compares these viruses through a shared endothelial-coagulative framework and proposes four partially overlapping vascular programs: endothelial activation, endothelial barrier failure, coagulation activation, and platelet quantitative or qualitative dysfunction. These programs are intended as a conceptual synthesis of convergent clinical, pathological, and experimental evidence rather than as a quantitative classification system. The framework is used not only to organize known mechanisms, but also to generate testable predictions for cross-virus biomarker panels, organ-specific endothelial models, and phenotype-guided clinical studies. Severe COVID-19 illustrates inflammation-driven endothelial activation, platelet-leukocyte interactions, complement amplification, NETosis, and immunothrombosis. Within the flavivirus group, DENV is used as the principal model for systemic vascular leakage, thrombocytopenia, and hemorrhagic risk; selected non-dengue flaviviruses illustrate how shared endothelial, platelet, and coagulation modules are redirected toward placental dysfunction (ZIKV), neuroinvasion (WNV and JEV), or hepatic failure and coagulopathy (YFV). Hantavirus infections provide a distinctive model of capillary leak syndrome in which non-lytic endothelial infection, immune-mediated injury, and permeability signaling converge on renal and pulmonary microvascular beds. A comparative view suggests that thrombotic, hemorrhagic, and leaky phenotypes are not mutually exclusive categories, but different outcomes of overlapping vascular programs shaped by viral tropism, vascular-bed specialization, immune activation, and host susceptibility. FAU - Beltrami, Silvia AU - Beltrami S AUID- ORCID: 0000-0002-5923-9265 AD - Department of Environmental and Prevention Sciences, University of Ferrara, 44121 Ferrara, Italy. FAU - Strazzabosco, Giovanni AU - Strazzabosco G AUID- ORCID: 0000-0001-7227-7939 AD - Department of Environmental and Prevention Sciences, University of Ferrara, 44121 Ferrara, Italy. FAU - Schiuma, Giovanna AU - Schiuma G AUID- ORCID: 0000-0003-4652-5550 AD - Department of Environmental and Prevention Sciences, University of Ferrara, 44121 Ferrara, Italy. FAU - Rizzo, Sabrina AU - Rizzo S AUID- ORCID: 0000-0002-9246-136X AD - Department of Environmental and Prevention Sciences, University of Ferrara, 44121 Ferrara, Italy. FAU - Baroni, Marcello AU - Baroni M AUID- ORCID: 0000-0002-2659-6362 AD - Department of Life Sciences, University of Ferrara, 44121 Ferrara, Italy. FAU - Bortolotti, Daria AU - Bortolotti D AUID- ORCID: 0000-0003-4496-9561 AD - Department of Environmental and Prevention Sciences, University of Ferrara, 44121 Ferrara, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 SB - IM MH - Humans MH - COVID-19/virology/physiopathology MH - SARS-CoV-2/physiology MH - Flavivirus/physiology/pathogenicity MH - Orthohantavirus/physiology/pathogenicity MH - *RNA Virus Infections/virology/physiopathology MH - *Communicable Diseases, Emerging/virology MH - Animals MH - Endothelium, Vascular/virology MH - Flavivirus Infections/virology/physiopathology MH - Hantavirus Infections/virology/physiopathology MH - Blood Platelets PMC - PMC13709913 OTO - NOTNLM OT - NETosis OT - complement OT - emerging RNA viruses OT - endothelial dysfunction OT - immunothrombosis OT - platelets OT - thromboinflammation OT - vascular leak OT - viral coagulopathy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/19 CRDT- 2026/10/27 10:47 PHST- 2026/08/24 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - v18101160 [pii] AID - viruses-18-01160 [pii] AID - 10.3390/v18101160 [doi] PST - epublish SO - Viruses. 2026 Oct 19;18(10):1160. doi: 10.3390/v18101160. PMID- 42944078 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Oct 19 TI - Synthetic Reconstruction and Characterization of Endogenous Bacteriophage Genomes from Desulfovibrio vulgaris. LID - 10.3390/v18101159 [doi] LID - 1159 AB - Microbiologically influenced corrosion (MIC) caused by sulfate-reducing bacteria (SRB) is a major challenge in industrial infrastructure, yet the limited availability of well-characterized bacteriophages has hindered the development of phage-based strategies for controlling these organisms. Here, we present an integrated genome-guided workflow for the discovery, validation, and synthetic reconstruction of endogenous prophages from the model sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough. Bioinformatic analysis using PHASTEST identified seven prophage-like regions distributed throughout the bacterial chromosome, including five classified as intact prophages. Ultraviolet (UV)-mediated induction followed by whole-genome sequencing of purified phage DNA experimentally validated four inducible prophage loci (Regions 1, 5, 6 and 7) detected in all three biological replicates, together with a fifth locus (Region 2) detected less consistently, demonstrating strong agreement between computational prediction and biological activity. Comparative analysis further showed that prophage functionality was not always correlated with computational completeness, as one computationally "questionable" region exhibited robust induction whereas another "intact" region remained inactive under the conditions tested. Based on these results, one highly inducible prophage was selected for synthetic engineering. Three progressively truncated synthetic prophage genomes (approximately 36, 33, and 29 kb) were assembled using long-range PCR and scarless isothermal DNA assembly and verified by Nanopore sequencing, which confirmed accurate reconstruction without detectable rearrangements. Following electroporation into D. vulgaris and UV induction, the engineered genomes produced reproducible localized turbid phenotypes rather than classical lytic plaques, indicating that the synthetic constructs remained biologically active but did not undergo efficient lytic propagation under the experimental conditions examined. Quantitative PCR analysis of representative structural and lytic genes in wild-type cells revealed coordinated transcriptional activation by 21 h after UV induction, defining a practical harvest time for subsequent characterization. Collectively, this work establishes an integrated computational and synthetic biology framework for engineering endogenous prophages from anaerobic SRB. The approach provides a foundation for investigating temperate phage biology in D. vulgaris and represents an important step toward the future development of engineered phage platforms for MIC control. FAU - Lim, Butaek AU - Lim B AUID- ORCID: 0000-0002-9027-0308 AD - Department of Bioengineering, University of California, Berkeley, CA 94720, USA. AD - Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd, Berkeley, CA 94720, USA. FAU - Fida, Tekle AU - Fida T AUID- ORCID: 0000-0003-2223-8522 AD - Gas Technology Institute, 1700 South Mount Prospect Rd, Des Plaines, IL 60018, USA. FAU - Crippen, Karen AU - Crippen K AD - Gas Technology Institute, 1700 South Mount Prospect Rd, Des Plaines, IL 60018, USA. FAU - Wilson, Rebekah AU - Wilson R AD - US Army Corps of Engineers, Champaign, IL 61820, USA. FAU - Lee, Seung-Wuk AU - Lee SW AUID- ORCID: 0000-0002-0501-8432 AD - Department of Bioengineering, University of California, Berkeley, CA 94720, USA. AD - Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Rd, Berkeley, CA 94720, USA. LA - eng GR - W9132T22C0023/United States Army Corps of Engineers/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (DNA, Viral) SB - IM MH - *Desulfovibrio vulgaris/virology/genetics MH - *Genome, Viral MH - *Prophages/genetics MH - Computational Biology MH - *Bacteriophages/genetics MH - Ultraviolet Rays MH - DNA, Viral/genetics MH - Virus Activation MH - Corrosion PMC - PMC13709724 OTO - NOTNLM OT - Desulfovibrio vulgaris OT - PHASTEST OT - bacteriophage OT - microbiologically influenced corrosion OT - phage genome engineering OT - prophage induction OT - sulfate-reducing bacteria OT - synthetic genome assembly COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/19 CRDT- 2026/10/27 10:47 PHST- 2026/08/30 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - v18101159 [pii] AID - viruses-18-01159 [pii] AID - 10.3390/v18101159 [doi] PST - epublish SO - Viruses. 2026 Oct 19;18(10):1159. doi: 10.3390/v18101159. PMID- 42944075 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Oct 18 TI - The Role of Viral Pathogens in the Promotion of Epithelial-Mesenchymal Transition and Neoplastic Cell Invasion. LID - 10.3390/v18101156 [doi] LID - 1156 AB - Epithelial-to-mesenchymal transition (EMT) is a multistep process in which stationary, polarized epithelial cells lose cell-to-cell adhesion and acquire a mesenchymal phenotype with migratory and invasive properties. Migrating EMT cells express both epithelial and mesenchymal markers, an intermediate hybrid state that allows them to retain some epithelial traits for survival while gaining mobility. After migration and metastasis to a secondary tumor site, EMT cells revert to an epithelial-like state via a mesenchymal-to-epithelial transition (MET) to establish new tumors. Cells undergoing EMT may also acquire cancer stem cell properties, leading to chemotherapy resistance and cancer recurrence. Accumulating evidence suggests that both oncogenic and non-oncogenic viruses can induce EMT. Among the oncogenic viruses, EMT has been reported in neoplastic cells infected with human papillomavirus, hepatitis B and C viruses, Epstein-Barr virus, and Kaposi sarcoma-associated herpesvirus. Non-oncogenic viruses, including human cytomegalovirus and human immunodeficiency virus, can also induce EMT in neoplastic cells. Furthermore, coinfection of neoplastic cells with two or more oncogenic viruses or oncogenic and non-oncogenic viruses may lead to synergistic and/or additive effects with respect to EMT induction. This review presents our current understanding of the molecular mechanisms underlying virus-induced EMT and the role of this transition in promoting neoplastic disease. FAU - Tugizov, Sharof AU - Tugizov S AUID- ORCID: 0000-0002-3743-4334 AD - Department of Medicine, School of Medicine, University of California-San Francisco, San Francisco, CA 94143, USA. LA - eng GR - R01 CA232887/CA/NCI NIH HHS/United States GR - R01 DE028129/DE/NIDCR NIH HHS/United States GR - R01DE028129/DE/NIDCR NIH HHS/United States PT - Journal Article PT - Review DEP - 20261018 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 SB - IM MH - *Epithelial-Mesenchymal Transition MH - Humans MH - *Oncogenic Viruses/physiology/pathogenicity MH - *Neoplasm Invasiveness MH - Animals MH - *Neoplasms/virology/pathology PMC - PMC13709368 OTO - NOTNLM OT - cancer invasion OT - epithelial-mesenchymal transition OT - neoplastic cells OT - virus COIS- The author declares no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/18 CRDT- 2026/10/27 10:47 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - v18101156 [pii] AID - viruses-18-01156 [pii] AID - 10.3390/v18101156 [doi] PST - epublish SO - Viruses. 2026 Oct 18;18(10):1156. doi: 10.3390/v18101156. PMID- 42944074 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Oct 17 TI - Immune Imprinting Across Respiratory Virus Infection, Vaccination, and Passive Immunization: Rethinking 'Original Antigenic Sin' in Influenza, SARS-CoV-2, and RSV. LID - 10.3390/v18101155 [doi] LID - 1155 AB - Immune imprinting shapes every subsequent response to antigenically related pathogens and is now central to designing updated vaccines for influenza, SARS-CoV-2, and respiratory syncytial virus (RSV). The label "Original Antigenic Sin" has framed imprinting as a fixed liability, but recent germinal-center, B-cell repertoire, and epidemiological data show that the effect is neither uniformly harmful nor irreversible. This review distinguishes cross-reactive immune memory from immune imprinting, defined here as a preferential bias toward the first-encountered antigen. Prior exposure can provide useful cross-reactive memory, but available evidence generally does not show that first-antigen bias itself improves protection relative to an equivalent unbiased memory response. We therefore use a hypothesis-generating conceptual framework to examine how antigenic distance, conserved-epitope accessibility, and exposure interval may influence both the usefulness of recalled memory and the extent to which imprinting restricts recruitment of responses to later antigens. The framework organizes existing observations and is not intended to predict imprinting outcomes ex ante. We discuss recent quantitative models that explicitly formalize competition between memory and de novo responses as complementary, pathogen-specific approaches. Across influenza and SARS-CoV-2, early exposure can establish cross-reactive memory associated with subsequent heterologous protection, while preferential recall of the priming antigen may limit responses to novel epitopes. For RSV, the effects of maternal vaccination and passive antibodies on subsequent active immune memory remain uncertain. Immunogen engineering, schedule adjustment, adjuvant selection, and cohort-tailored strategies may broaden pre-existing memory toward conserved protective epitopes and reduce restrictive first-antigen bias. FAU - Szuster-Ciesielska, Agnieszka AU - Szuster-Ciesielska A AUID- ORCID: 0000-0003-4046-1920 AD - Department of Virology and Immunology, Institute of Biological Sciences, Maria Curie-Sklodowska University, Akademicka 19, 20-032 Lublin, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261017 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (Influenza Vaccines) RN - 0 (Antigens, Viral) RN - 0 (Respiratory Syncytial Virus Vaccines) SB - IM MH - Humans MH - Immunologic Memory MH - *Respiratory Syncytial Virus Infections/immunology/prevention & control MH - *SARS-CoV-2/immunology MH - Cross Reactions/immunology MH - *Influenza, Human/immunology/prevention & control MH - Vaccination MH - *COVID-19/immunology/prevention & control MH - Immunization, Passive MH - Animals MH - Influenza Vaccines/immunology MH - Antigens, Viral/immunology MH - Respiratory Syncytial Viruses/immunology MH - Respiratory Syncytial Virus Vaccines/immunology PMC - PMC13709708 OTO - NOTNLM OT - SARS-CoV-2 spike OT - back-boosting OT - germinal center OT - hemagglutinin OT - immune imprinting OT - original antigenic sin OT - respiratory syncytial virus OT - universal influenza vaccine COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/17 CRDT- 2026/10/27 10:47 PHST- 2026/08/18 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - v18101155 [pii] AID - viruses-18-01155 [pii] AID - 10.3390/v18101155 [doi] PST - epublish SO - Viruses. 2026 Oct 17;18(10):1155. doi: 10.3390/v18101155. PMID- 42944072 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Oct 17 TI - From Defenders to Hosts: The Arm-in-Arm Expansion of KRAB-Zinc Finger Gene Repertoires and Retroviral Integrations in Mammals. LID - 10.3390/v18101153 [doi] LID - 1153 AB - KRAB-zinc finger proteins (KZFPs) constitute one of the largest and most rapidly evolving families of transcription factors in vertebrates, with particularly extensive expansion and diversification in mammals. While several KZFPs are conserved across species, numerous lineage- and species-specific genes have emerged during mammalian evolution, with a large fraction recognizing and repressing transposable elements (TEs), and particularly endogenous retroviruses (ERVs). The evolutionary relationship between KZFPs and ERVs has traditionally been interpreted through an "arms-race" model, in which recurrent retroviral invasions drive the emergence of new KZFP specificities, while retroelements evolve to escape host repression. This review examines the evidence linking the evolutionary dynamics of KZFPs and ERVs and discusses how their relationship may extend beyond reciprocal adaptation at the level of sequence recognition. In particular, recent comparative genomic analyses reveal that young KZFP genes are frequently organized in highly repetitive genomic clusters that are themselves enriched in young ERVs. Structural variation and segmental duplication within these regions can simultaneously expand KZFP and ERV copy numbers, providing a potential genomic mechanism for their concerted diversification. These observations motivate an "arm-in-arm" model, in which KZFPs and ERVs are not only antagonistic evolutionary partners but can also become physically and evolutionarily intertwined within the same genomic environments. The possibility that KZFP clusters may have a dual role in the evolutionary fate of retroviral sequences is also considered: while KZFP-mediated repression can limit the deleterious effects of newly integrated retroelements and facilitate their persistence, the repetitive and structurally dynamic nature of these loci may create opportunities for subsequent duplication and diversification. Together, these observations broaden the classical arms-race framework and highlight how the interplay between retroviral invasion, host defense, genomic architecture, and sequence cooption can contribute to the generation of genomic and regulatory diversity. FAU - Bruno, Melania AU - Bruno M AUID- ORCID: 0000-0002-8401-7744 AD - Independent Researcher, 80053 Castellammare di Stabia, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261017 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (Repressor Proteins) RN - 0 (DNA Transposable Elements) SB - IM MH - Animals MH - *Zinc Fingers/genetics MH - *Endogenous Retroviruses/genetics/physiology MH - Evolution, Molecular MH - *Mammals/genetics/virology MH - *Virus Integration MH - Humans MH - *Repressor Proteins/genetics MH - DNA Transposable Elements PMC - PMC13709654 OTO - NOTNLM OT - KRAB-zinc finger proteins OT - endogenous retroviruses OT - repetitive gene clusters OT - transposable elements COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/17 CRDT- 2026/10/27 10:47 PHST- 2026/08/14 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - v18101153 [pii] AID - viruses-18-01153 [pii] AID - 10.3390/v18101153 [doi] PST - epublish SO - Viruses. 2026 Oct 17;18(10):1153. doi: 10.3390/v18101153. PMID- 42944066 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Oct 16 TI - Generation and Characterization of Single-Domain Antibodies Against the D-Antigen of Sabin Poliovirus Types 2 and 3. LID - 10.3390/v18101147 [doi] LID - 1147 AB - A panel of monoclonal nanobodies (VHH) specific to poliovirus Sabin strains types 2 and 3 has been generated and characterized. For poliovirus Sabin type 2, a panel of four serotype-specific single-domain antibodies was obtained. Notably, two of these antibodies, S2-C5 and S2-D5, were able to discriminate between D- and H-antigens. Three nanobodies specifically neutralized poliovirus Sabin type 2 and exhibited no neutralizing activity against the homologous wild-type MEF-1 strain or other poliovirus serotypes. Among the four nanobodies selected against Sabin type 3, S3-A2 and S3-G1 demonstrated high serotype specificity in ELISAs. When used as a capture antibody in the ELISA, S3-G1 showed preferential binding to D-antigen over H-antigen. However, the type 3 nanobodies did not display virus-neutralizing activity. The obtained antibodies represent promising tools for improving existing immunoassays based on polyclonal antibodies by increasing D-antigen specificity and may facilitate the development of next-generation ELISA systems for the quality control of inactivated polio vaccines. FAU - Ermakova, Maya AU - Ermakova M AUID- ORCID: 0000-0002-8229-7818 AD - Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products, Russian Academy of Sciences (Institute of Poliomyelitis), 108819 Moscow, Russia. FAU - Ivanov, Sergey AU - Ivanov S AD - Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products, Russian Academy of Sciences (Institute of Poliomyelitis), 108819 Moscow, Russia. FAU - Shmeleva, Olga AU - Shmeleva O AD - Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products, Russian Academy of Sciences (Institute of Poliomyelitis), 108819 Moscow, Russia. FAU - Borisenko, Nadezhda AU - Borisenko N AD - Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products, Russian Academy of Sciences (Institute of Poliomyelitis), 108819 Moscow, Russia. FAU - Zyrina, Anna AU - Zyrina A AUID- ORCID: 0000-0002-3675-8361 AD - Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products, Russian Academy of Sciences (Institute of Poliomyelitis), 108819 Moscow, Russia. FAU - Levin, Igor AU - Levin I AD - Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products, Russian Academy of Sciences (Institute of Poliomyelitis), 108819 Moscow, Russia. FAU - Chumakov, Stepan AU - Chumakov S AUID- ORCID: 0000-0003-2866-5277 AD - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences (IBCh RAS), 117997 Moscow, Russia. FAU - Shishova, Anna AU - Shishova A AD - Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products, Russian Academy of Sciences (Institute of Poliomyelitis), 108819 Moscow, Russia. AD - Institute for Translational Medicine and Biotechnology, First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia. FAU - Ivin, Yury AU - Ivin Y AUID- ORCID: 0000-0003-0995-7944 AD - Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products, Russian Academy of Sciences (Institute of Poliomyelitis), 108819 Moscow, Russia. FAU - Piniaeva, Anastasia AU - Piniaeva A AUID- ORCID: 0000-0001-5381-2393 AD - Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products, Russian Academy of Sciences (Institute of Poliomyelitis), 108819 Moscow, Russia. FAU - Siniugina, Alexandra AU - Siniugina A AD - Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products, Russian Academy of Sciences (Institute of Poliomyelitis), 108819 Moscow, Russia. FAU - Ishmukhametov, Aydar AU - Ishmukhametov A AD - Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products, Russian Academy of Sciences (Institute of Poliomyelitis), 108819 Moscow, Russia. AD - Institute for Translational Medicine and Biotechnology, First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia. LA - eng GR - FNZG-2026-0007/Government of Russia/ GR - FNZG-2024-0004/Government of Russia/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (Antibodies, Viral) RN - 0 (Antigens, Viral) RN - 0 (Single-Domain Antibodies) RN - 0 (Antibodies, Neutralizing) SB - IM MH - *Poliovirus/immunology/classification MH - *Antibodies, Viral/immunology/isolation & purification MH - Humans MH - Enzyme-Linked Immunosorbent Assay MH - *Antigens, Viral/immunology MH - *Single-Domain Antibodies/immunology/isolation & purification MH - Antibodies, Neutralizing/immunology MH - Animals MH - Neutralization Tests PMC - PMC13709865 OTO - NOTNLM OT - D-antigen OT - ELISA test OT - Sabin OT - VHH OT - neutralization OT - phage display OT - poliovirus COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/16 CRDT- 2026/10/27 10:47 PHST- 2026/07/31 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - v18101147 [pii] AID - viruses-18-01147 [pii] AID - 10.3390/v18101147 [doi] PST - epublish SO - Viruses. 2026 Oct 16;18(10):1147. doi: 10.3390/v18101147. PMID- 42944065 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Oct 15 TI - Respiratory Syncytial Virus Infection: Enhanced Reactive Oxygen Species Production and Weakened Antioxidant Defenses Worsen Disease Severity. LID - 10.3390/v18101146 [doi] LID - 1146 AB - Respiratory syncytial virus (RSV) is a leading cause of severe respiratory infections in infants and young children globally. The severity of RSV-associated diseases is often ascribed to a dysregulated immune response, characterized by increased secretion of proinflammatory proteins and ineffective T helper type 2 (Th2) cell activity. The host's primary defense against RSV relies on a well-coordinated innate immune response initiated by resident airway epithelial cells, the first target of RSV infection. These cells respond to viral invasion by generating reactive oxygen species (ROS), activating the production of Type I and III interferons, and releasing proinflammatory cytokines and chemokines. Moderate levels of ROS act as key molecules that regulate antiviral signaling, enhancing the host's ability to recognize and eliminate invading pathogens. However, severe RSV infection disrupts cellular homeostasis triggering excessive ROS production, while simultaneously weakening the host's antioxidant defenses. This redox imbalance subverts the activity of key cellular defensive mechanisms, ultimately increasing viral replication, and worsening RSV infection severity. Modulation of oxidative stress may offer an innovative approach in managing the progression and outcome of RSV infection. FAU - Sacco, Oliviero AU - Sacco O AD - Pediatric Pulmonology and Respiratory Endoscopy Unit, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Istituto Giannina Gaslini, 16147 Genoa, Italy. FAU - Borgia, Paola AU - Borgia P AUID- ORCID: 0000-0002-3520-915X AD - Pediatric Pulmonology and Respiratory Endoscopy Unit, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Istituto Giannina Gaslini, 16147 Genoa, Italy. FAU - Rossi, Giorgia M AU - Rossi GM AD - Department of Internal Medicine, Universita di Genova, 16132 Genova, Italy. FAU - Florio, Angelo AU - Florio A AD - Pediatric Pulmonology and Respiratory Endoscopy Unit, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Istituto Giannina Gaslini, 16147 Genoa, Italy. FAU - Rossi, Giovanni A AU - Rossi GA AD - Pediatric Pulmonology and Respiratory Endoscopy Unit, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Istituto Giannina Gaslini, 16147 Genoa, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (Reactive Oxygen Species) RN - 0 (Antioxidants) RN - 0 (Cytokines) SB - IM MH - *Respiratory Syncytial Virus Infections/immunology/virology/metabolism/pathology MH - *Reactive Oxygen Species/metabolism MH - Humans MH - Immunity, Innate MH - Oxidative Stress MH - *Antioxidants/metabolism MH - *Respiratory Syncytial Virus, Human/immunology MH - Animals MH - Virus Replication MH - Host-Pathogen Interactions/immunology MH - Severity of Illness Index MH - Epithelial Cells/virology/immunology MH - Cytokines/metabolism PMC - PMC13709499 OTO - NOTNLM OT - airway epithelial cells OT - bronchiolitis OT - innate immune response OT - innate lymphoid cell2 OT - oxidative stress OT - viral infections COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/15 CRDT- 2026/10/27 10:47 PHST- 2026/07/29 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - v18101146 [pii] AID - viruses-18-01146 [pii] AID - 10.3390/v18101146 [doi] PST - epublish SO - Viruses. 2026 Oct 15;18(10):1146. doi: 10.3390/v18101146. PMID- 42944064 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Oct 15 TI - Phage Receptor-Binding Proteins for Targeted Silver Nanoparticle Delivery: Design and Evaluation. LID - 10.3390/v18101145 [doi] LID - 1145 AB - Antimicrobial resistance has renewed interest in silver nanoparticles (AgNPs), but limited pathogen selectivity complicates their therapeutic use. Bacteriophage receptor-binding proteins (RBPs) recognize bacterial surface receptors and offer candidate targeting modules for AgNP delivery. However, evidence from intact-phage composites and non-silver carriers cannot establish the performance of isolated RBP-AgNP systems. In this review, we link RBP architecture and receptor recognition to ligand selection, orientation and multivalent display on AgNPs. We outline workflows for RBP discovery and receptor assignment, review conjugation strategies, and examine how Ag(+) release and oxidative stress contribute to antibacterial activity. We propose a dual-endpoint framework to quantify nanoparticle enrichment near target bacteria and test for bactericidal gain using receptor-level controls, matched total silver doses and measurements of silver release. We discuss protein-corona masking, receptor variation and activity retention in complex infection settings, including biofilms, alongside manufacturing reproducibility. Translation further requires formulation-specific assessment of in vivo exposure, host tolerance, environmental fate and ecological risk. This framework defines the evidence needed to determine whether RBP-guided delivery improves antibacterial efficacy and safety. FAU - He, Qing AU - He Q AD - Military Medical Sciences Academy, Academy of Military Sciences, Tianjin 300050, China. FAU - Yang, Xiaobo AU - Yang X AD - Military Medical Sciences Academy, Academy of Military Sciences, Tianjin 300050, China. FAU - Qiu, Zhigang AU - Qiu Z AUID- ORCID: 0000-0003-1199-3397 AD - Military Medical Sciences Academy, Academy of Military Sciences, Tianjin 300050, China. LA - eng GR - 24JCZDJC00940/Natural Science Foundation of Tianjin City/ PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 3M4G523W1G (Silver) RN - 0 (Receptors, Virus) RN - 0 (Anti-Bacterial Agents) RN - 0 (Viral Proteins) SB - IM MH - *Silver/chemistry/pharmacology MH - *Metal Nanoparticles/chemistry MH - *Bacteriophages/metabolism MH - *Receptors, Virus/metabolism/chemistry MH - *Anti-Bacterial Agents/pharmacology/chemistry MH - Bacteria/drug effects MH - Humans MH - *Drug Delivery Systems MH - *Viral Proteins/metabolism/chemistry PMC - PMC13709587 OTO - NOTNLM OT - antimicrobial resistance OT - biofilm-associated infections OT - receptor-binding proteins OT - silver nanoparticles OT - targeted delivery COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/15 CRDT- 2026/10/27 10:47 PHST- 2026/09/27 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - v18101145 [pii] AID - viruses-18-01145 [pii] AID - 10.3390/v18101145 [doi] PST - epublish SO - Viruses. 2026 Oct 15;18(10):1145. doi: 10.3390/v18101145. PMID- 42944053 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Oct 13 TI - Evaluation of Anti-HIV-1 HSPC-Based Gene Therapy with Negative Selection Marker in Pigtail Macaques. LID - 10.3390/v18101133 [doi] LID - 1133 AB - Despite significant medical progress, HIV-1 remains a critical global health crisis. While combination antiretroviral therapy (cART) effectively suppresses viremia, it is not curative due to the persistence of latent viral reservoirs. Autologous hematopoietic stem and progenitor cell (HSPC) gene therapy offers a promising avenue toward sustained, drug-free remission. In this study, we investigated the feasibility and safety of a multifunctional "defend and attack" HSPC gene therapy strategy in the pigtail macaque (Macaca nemestrina) model. The species-optimized lentiviral vector, PM-MNDU-anti-HIV-1-huEGFRt, carries four therapeutic payloads: a pigtail macaque-specific CCR5 shRNA (PM-CCR5sh1005), a CD4-based chimeric antigen receptor (D1D2CAR), a C46 fusion inhibitor peptide, and a truncated human epidermal growth factor receptor (huEGFRt) serving as a cell-surface tracking marker and anti-EGFR antibody Cetuximab (CTX)-mediated negative selection marker. Following busulfan conditioning, autologous transplantation of vector-modified HSPCs in two pigtail macaques resulted in safe hematopoietic recovery. Both animals achieved sustained, multilineage gene marking in peripheral blood and lymphoid tissues without adverse effects. In one animal, upon Simian-Human Immunodeficiency Virus (SHIV) challenge, vector-modified cells underwent robust, antigen-driven multilineage expansion across both lymphoid and myeloid compartments, which contracted during cART-induced viral suppression and rapidly rebounded following treatment interruption. Administration of CTX demonstrated partial, transient reduction in huEGFRt(+) cells, accompanied with a reciprocal increase in plasma viral load. These findings support the feasibility of evaluating a multi-pronged anti-HIV HSPC gene therapy strategy with a negative selection marker in a non-human primate (NHP) model. FAU - Zhang, Jian AU - Zhang J AD - University of California Los Angeles (UCLA) AIDS Institute, Los Angeles, CA 90024, USA. AD - UCLA Joe C. Wen School of Nursing, UCLA, Los Angeles, CA 90095, USA. FAU - Murray, Jason AU - Murray J AUID- ORCID: 0000-0002-5551-2607 AD - Stem Cell and Gene Therapy Program, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA. FAU - Mu, Wenli AU - Mu W AD - University of California Los Angeles (UCLA) AIDS Institute, Los Angeles, CA 90024, USA. AD - Department of Medicine, Division of Hematology and Oncology, David Geffen School of Medicine, UCLA, Los Angeles, CA 90095, USA. FAU - Venugopal, Surya AU - Venugopal S AD - University of California Los Angeles (UCLA) AIDS Institute, Los Angeles, CA 90024, USA. AD - UCLA Joe C. Wen School of Nursing, UCLA, Los Angeles, CA 90095, USA. FAU - Yim, Ella AU - Yim E AD - Department of Microbiology, Immunology, and Molecular Genetics, UCLA, Los Angeles, CA 90095, USA. FAU - Einhaus, Teresa AU - Einhaus T AUID- ORCID: 0000-0002-7048-6750 AD - Stem Cell and Gene Therapy Program, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA. FAU - Radtke, Stefan AU - Radtke S AUID- ORCID: 0000-0002-2842-4820 AD - Stem Cell and Gene Therapy Program, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA. FAU - Peterson, Christopher W AU - Peterson CW AD - Stem Cell and Gene Therapy Program, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA. AD - Department of Medicine, University of Washington, Seattle, WA 98195, USA. FAU - Kiem, Hans-Peter AU - Kiem HP AD - Stem Cell and Gene Therapy Program, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA. AD - Department of Medicine, University of Washington, Seattle, WA 98195, USA. FAU - An, Dong Sung AU - An DS AUID- ORCID: 0000-0002-7020-9470 AD - University of California Los Angeles (UCLA) AIDS Institute, Los Angeles, CA 90024, USA. AD - UCLA Joe C. Wen School of Nursing, UCLA, Los Angeles, CA 90095, USA. LA - eng GR - P51 OD010425/Washington National Biomedical Research Center/ GR - P30 CA015704/Fred Hutchinson/University of Washington/Seattle Children's Cancer Consortium/ GR - James B. Pendleton Charitable Trust/ GR - U42 OD011123/OD/NIH HHS/United States GR - AI152501/National Institute of Allergy and Infectious Diseases/ GR - U19 AI149504/AI/NIAID NIH HHS/United States GR - McCarthy Family Foundation/ GR - U19 AI149504/National Institute of Allergy and Infectious Diseases/ GR - U54 DK106829/DK/NIDDK NIH HHS/United States GR - P51 OD010425/OD/NIH HHS/United States GR - P30 CA015704/CA/NCI NIH HHS/United States GR - P30 AI152501/AI/NIAID NIH HHS/United States GR - U42 OD011123/Washington National Biomedical Research Center/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (Receptors, CCR5) RN - 0 (C46 HIV-1 fusion inhibitory peptide) RN - 0 (Recombinant Fusion Proteins) SB - IM MH - Animals MH - Macaca nemestrina MH - *Genetic Therapy/methods MH - Genetic Vectors MH - *Hematopoietic Stem Cells/virology/metabolism MH - *HIV-1/genetics MH - *HIV Infections/therapy/virology MH - Lentivirus/genetics MH - *Hematopoietic Stem Cell Transplantation/methods MH - Gene Therapy Agents MH - Receptors, CCR5/genetics MH - Viral Load MH - Humans MH - Recombinant Fusion Proteins PMC - PMC13709364 OTO - NOTNLM OT - C46 fusion inhibitor OT - CCR5 shRNA OT - Cetuximab OT - HIV-1 OT - SHIV OT - chimeric antigen receptor (CAR) OT - hematopoietic stem and progenitor cell (HSPC) gene thCherapy OT - huEGFRt OT - lentiviral vector OT - non-human primate (NHP) COIS- H.-P.K. has received support as the inaugural recipient of the Jose Carreras/E. Donnall Thomas Endowed Chair for Cancer Research and the Stephanus Family Endowed Chair for Cell and Gene Therapy and is or was a consultant to and has or had ownership interests with Rocket Pharmaceuticals, Homology Medicines, Vor Biopharma, and Ensoma Inc. S.R. is a consultant to Forty Seven Inc. (Gilead Sciences) and Ensoma Inc. D.S.A. holds US patent for CCR5sh1005. The remaining authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/13 CRDT- 2026/10/27 10:47 PHST- 2026/08/25 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:47 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - v18101133 [pii] AID - viruses-18-01133 [pii] AID - 10.3390/v18101133 [doi] PST - epublish SO - Viruses. 2026 Oct 13;18(10):1133. doi: 10.3390/v18101133. PMID- 42944043 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Oct 10 TI - An Inter-Subgenotypic PEDV Recombinant Harboring a Complete Structural Protein Cassette from the GIIb Lineage. LID - 10.3390/v18101123 [doi] LID - 1123 AB - Porcine epidemic diarrhea virus (PEDV) poses a persistent threat to the global swine industry through the continual emergence of variants driven by mutation and recombination. Here, we report the isolation and genomic characterization of PEDV2-5, an inter-subgenotypic recombinant isolated from clinical samples collected in Jiangxi Province, China. Phylogenetic analysis based on both the complete genome and the full-length S gene placed PEDV2-5 within the GIIb subgenotypes, clustering closely with FL201 and GD-1. Recombination detection using seven independent algorithms in RDP5, corroborated by SimPlot and phylogenetic incongruence analysis, identified PEDV2-5 as a recombinant between a GIIa major parental-like (PEDV-LY) and a GIIb minor parental-like (GD-1), generating a 7931-nucleotide fragment (nt 20060-27990) that spans the complete structural protein cassette (S, ORF3, E, M, N) and the 3'-terminal portion of ORF1b. The recombinant has replication-competent Vero-E6 cells, reaching a peak titer of 5.03 log10 TCID(50)/mL at 36 h post-infection. Amino acid analysis revealed 98.49% identity between the PEDV2-5 S protein and the GIIb strain AJ1102, together with 13 unique substitutions and a "KKKG" insertion (residues 361-364), including two substitutions within neutralizing epitopes (A517F in COE and Y1374C in 2C10). Relative to AJ1102, the neutralizing epitopes were nearly completely conserved, whereas extensive divergence was observed against the classical GIa strain CV777. These findings highlight the importance of continued whole-genome surveillance for monitoring the emergence and spread of recombinant PEDV lineages and for informing future vaccine antigen-selection strategies. FAU - Chen, Yue AU - Chen Y AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. FAU - Yuan, Lin AU - Yuan L AD - Beijing Sino-science Gene Technology Co., Ltd., Beijing 102629, China. FAU - Wang, Wenqiang AU - Wang W AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. FAU - Sun, Dehu AU - Sun D AD - Beijing Sino-science Gene Technology Co., Ltd., Beijing 102629, China. FAU - Chen, Lulu AU - Chen L AD - Beijing Sino-science Gene Technology Co., Ltd., Beijing 102629, China. FAU - Zhang, Jie AU - Zhang J AD - Beijing Sino-science Gene Technology Co., Ltd., Beijing 102629, China. FAU - Guo, Zihan AU - Guo Z AD - Beijing Sino-science Gene Technology Co., Ltd., Beijing 102629, China. FAU - Yan, Pinnan AU - Yan P AD - Pudao (Beijing) Standard Technology Co., Ltd., Beijing 102629, China. FAU - Chen, Yana AU - Chen Y AD - Pudao (Beijing) Standard Technology Co., Ltd., Beijing 102629, China. FAU - Lv, Junxia AU - Lv J AD - Pudao (Beijing) Standard Technology Co., Ltd., Beijing 102629, China. FAU - Ma, Yan AU - Ma Y AD - Pudao (Beijing) Standard Technology Co., Ltd., Beijing 102629, China. FAU - Zhu, Zhenbang AU - Zhu Z AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. FAU - Wen, Wei AU - Wen W AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. FAU - Tian, Kegong AU - Tian K AD - National Research Center for Veterinary Medicine, Luoyang 471000, China. FAU - Li, Xiangdong AU - Li X AUID- ORCID: 0000-0002-4635-1602 AD - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. AD - Jiangsu Key Laboratory of Zoonosis, Jiangsu Interdisciplinary Center for Zoonoses and Biosafety, Yangzhou University, Yangzhou 225009, China. LA - eng PT - Journal Article DEP - 20261010 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 SB - IM MH - *Porcine epidemic diarrhea virus/genetics/classification/isolation & purification MH - Animals MH - Phylogeny MH - Genome, Viral MH - *Recombination, Genetic MH - Swine MH - *Swine Diseases/virology MH - *Coronavirus Infections/veterinary/virology MH - Genotype MH - China MH - Chlorocebus aethiops MH - Vero Cells PMC - PMC13709889 OTO - NOTNLM OT - PEDV OT - complete genome OT - molecular characterization OT - phylogenetic analysis OT - recombination OT - spike protein COIS- Lin Yuan, Dehu Sun, Lulu Chen, Jie Zhang, and Zihan Guo were employed by the company Beijing Sino-science Gene Technology Co., Ltd. Pinnan Yan, Yana Chen, Junxia Lv, and Yan Ma were employed by the company Pudao (Beijing) Standard Technology Co., Ltd. These companies had no influence on this study. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. This research also received no commercial funding from any company. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/10 CRDT- 2026/10/27 10:46 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - v18101123 [pii] AID - viruses-18-01123 [pii] AID - 10.3390/v18101123 [doi] PST - epublish SO - Viruses. 2026 Oct 10;18(10):1123. doi: 10.3390/v18101123. PMID- 42944027 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Oct 7 TI - Clinical Profile of RSV Breakthrough Infections After Nirsevimab Introduction. LID - 10.3390/v18101107 [doi] LID - 1107 AB - The introduction of universal prophylaxis with nirsevimab for all children in their first epidemic season has substantially reduced the burden of respiratory syncytial virus (RSV). However, a small number of breakthrough infections continue to occur despite immunization, mostly in previously healthy infants, and these cases remain incompletely characterized. We conducted a retrospective study across three epidemic seasons at a third-level hospital, including 536 RSV-positive children under 12 months of age hospitalised for bronchiolitis. Immunised infants with breakthrough infection showed a higher prevalence of prematurity and comorbidities than unimmunised infants, and were more frequently represented in the 9-12-month age group, with a median of 291 days since nirsevimab administration. Immunised infants had significantly lower odds of requiring supplemental oxygen (aOR 0.33, 95% CI 0.19-0.57, p < 0.001). However, once oxygen therapy was required, immunisation status did not independently predict the need for advanced ventilatory support. Severe breakthrough cases requiring invasive mechanical ventilation occurred even among infants without traditional risk factors. These findings confirm the substantial real-world impact of universal nirsevimab prophylaxis while underscoring the need for continued clinical vigilance and further research into the mechanisms underlying breakthrough disease. FAU - Vittucci, Anna Chiara AU - Vittucci AC AUID- ORCID: 0000-0002-1164-5563 AD - General Pediatric and Infectious Disease Unit, Pediatric Emergency Medicine, Bambino Gesu Children's Hospital, IRCCS, 00165 Rome, Italy. FAU - Antilici, Livia AU - Antilici L AD - General Pediatric and Infectious Disease Unit, Pediatric Emergency Medicine, Bambino Gesu Children's Hospital, IRCCS, 00165 Rome, Italy. FAU - Salvatori, Alessandra AU - Salvatori A AUID- ORCID: 0000-0002-6289-2249 AD - General Pediatric and Infectious Disease Unit, Pediatric Emergency Medicine, Bambino Gesu Children's Hospital, IRCCS, 00165 Rome, Italy. FAU - De Rose, Domenico Umberto AU - De Rose DU AUID- ORCID: 0000-0002-9076-9838 AD - Neonatal Intensive Care Unit, Bambino Gesu Children's Hospital, IRCCS, 00165 Rome, Italy. FAU - Scutari, Rossana AU - Scutari R AD - Microbiology and Diagnostic Immunology Unit, Bambino Gesu Children's Hospital, IRCCS, 00165 Rome, Italy. FAU - Di Maio, Velia Chiara AU - Di Maio VC AD - Microbiology and Diagnostic Immunology Unit, Bambino Gesu Children's Hospital, IRCCS, 00165 Rome, Italy. FAU - Rotondi Aufiero, Lelia AU - Rotondi Aufiero L AD - General Pediatric and Infectious Disease Unit, Pediatric Emergency Medicine, Bambino Gesu Children's Hospital, IRCCS, 00165 Rome, Italy. FAU - Pisani, Mara AU - Pisani M AUID- ORCID: 0000-0003-3779-8557 AD - General Pediatric and Infectious Disease Unit, Pediatric Emergency Medicine, Bambino Gesu Children's Hospital, IRCCS, 00165 Rome, Italy. FAU - Musolino, Anna Maria AU - Musolino AM AD - General Pediatric and Infectious Disease Unit, Pediatric Emergency Medicine, Bambino Gesu Children's Hospital, IRCCS, 00165 Rome, Italy. FAU - Dotta, Andrea AU - Dotta A AUID- ORCID: 0000-0002-1260-6427 AD - Neonatal Intensive Care Unit, Bambino Gesu Children's Hospital, IRCCS, 00165 Rome, Italy. FAU - Perno, Carlo Federico AU - Perno CF AD - Microbiology and Diagnostic Immunology Unit, Bambino Gesu Children's Hospital, IRCCS, 00165 Rome, Italy. FAU - Villani, Alberto AU - Villani A AUID- ORCID: 0000-0002-9120-0424 AD - General Pediatric and Infectious Disease Unit, Pediatric Emergency Medicine, Bambino Gesu Children's Hospital, IRCCS, 00165 Rome, Italy. AD - Systems Medicine Departments, Tor Vergata University of Rome, 00133 Rome, Italy. LA - eng GR - Ministry of Health/ PT - Journal Article DEP - 20261007 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (Antiviral Agents) RN - 0 (Antibodies, Monoclonal, Humanized) SB - IM MH - Humans MH - *Respiratory Syncytial Virus Infections/prevention & control/epidemiology/drug therapy/virology MH - Breakthrough Infections MH - Retrospective Studies MH - Infant MH - Male MH - Female MH - *Antiviral Agents/therapeutic use/administration & dosage MH - *Antibodies, Monoclonal, Humanized/therapeutic use/administration & dosage MH - Respiratory Syncytial Virus, Human/drug effects/immunology MH - Infant, Newborn MH - Risk Factors MH - Bronchiolitis/virology PMC - PMC13709675 OTO - NOTNLM OT - RSV OT - breakthrough infections OT - bronchiolitis OT - immunoprophylaxis OT - nirsevimab OT - universal prophylaxis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/07 CRDT- 2026/10/27 10:46 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - v18101107 [pii] AID - viruses-18-01107 [pii] AID - 10.3390/v18101107 [doi] PST - epublish SO - Viruses. 2026 Oct 7;18(10):1107. doi: 10.3390/v18101107. PMID- 42944017 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Oct 3 TI - Viral Etiology of Sudden Sensorineural Hearing Loss: Historical Development and Current Evidence. LID - 10.3390/v18101097 [doi] LID - 1097 AB - Sudden sensorineural hearing loss (SSNHL) is an otological emergency with complex and heterogeneous etiologies, and most cases remain unexplained and are therefore classified as idiopathic. Viral infection has long been considered an important potential cause of SSNHL; however, the mechanisms underlying virus-associated hearing loss and the causal relationship between viral infection and SSNHL have not been fully elucidated. Current evidence suggests that viruses may induce acute hearing loss through direct invasion of the inner ear, reactivation of latent viruses, immune-mediated injury, and cellular stress and inflammatory responses. This review describes the major viruses associated with SSNHL and their epidemiological and clinical characteristics; summarizes evidence from serological testing, molecular virology, imaging, temporal bone histopathology, and animal studies; and discusses recent advances in the pathogenic mechanisms, etiological diagnosis, and antiviral treatment of virus-associated SSNHL. This review aims to systematically clarify the current evidence and controversies surrounding the viral etiology of SSNHL and to provide a reference for etiological identification, patient stratification, and targeted treatment of virus-associated SSNHL. FAU - Zhao, Shujie AU - Zhao S AD - Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. FAU - Liu, Xiaozhou AU - Liu X AUID- ORCID: 0009-0002-3437-9568 AD - Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. FAU - Shi, Xinyu AU - Shi X AUID- ORCID: 0009-0008-7113-5179 AD - Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. FAU - Zhao, Zhengdong AU - Zhao Z AD - Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. FAU - Sun, Yu AU - Sun Y AUID- ORCID: 0000-0002-1771-3715 AD - Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. AD - Institute of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. AD - Hubei Province Clinical Research Center for Deafness and Vertigo, Wuhan 430022, China. AD - Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan 430022, China. LA - eng GR - 82430035/National Natural Science Foundation of China/ GR - 2024YFC2511100 /2024YFC2511101, 2021YFF0702303, 2021YFF0702301/Ministry of Science and Technology of the People's Republic of China/ GR - 2024BRA019/Ministry of Finance of the People's Republic of China/ PT - Historical Article PT - Journal Article PT - Review DEP - 20261003 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 SB - IM MH - Animals MH - Humans MH - *Hearing Loss, Sensorineural/diagnosis/history/virology MH - *Hearing Loss, Sudden/history/virology MH - *Virus Diseases/complications/virology PMC - PMC13709506 OTO - NOTNLM OT - antiviral therapy OT - etiological diagnosis OT - inner-ear injury OT - sudden sensorineural hearing loss OT - viral infection OT - viral reactivation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/03 CRDT- 2026/10/27 10:46 PHST- 2026/09/05 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - v18101097 [pii] AID - viruses-18-01097 [pii] AID - 10.3390/v18101097 [doi] PST - epublish SO - Viruses. 2026 Oct 3;18(10):1097. doi: 10.3390/v18101097. PMID- 42944007 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Oct 1 TI - Study of Molecular Genetic Characteristics of Influenza A(H1N1) Viruses Identified During Monitoring in Kazakhstan (2023-2026). LID - 10.3390/v18101087 [doi] LID - 1087 AB - BACKGROUND/OBJECTIVES: Seasonal influenza threatens global public health through rapid mutations. This study evaluated the prevalence and molecular-genetic characteristics of seasonal influenza viruses circulating in Kazakhstan during the 2023-2026 epidemic seasons. METHODS: Nasopharyngeal swabs (n = 1756) and serum samples (n = 718) were screened using RT-qPCR, HI, and ELISA assays. Selected A(H1N1)pdm09 isolates underwent full-length HA and NA gene next-generation sequencing, followed by phylogenetic and phenotypic analyses. RESULTS: Influenza A(H3N2) strains dominated in Kazakhstan during the 2023-2024 and 2025-2026 seasons. Conversely, the 2024-2025 season showed a shifting landscape, characterized by co-circulation of influenza B and A(H1N1)pdm09 strains belonging to genetic clade 6B.1A.5a.2a (subclades C.1.9 and C.1.9.3). Mutational mapping revealed ongoing in situ microevolution adjacent to the receptor-binding site (HA-R222K). Genomic screening of the NA gene identified critical I223R and S247N mutations traditionally linked to resistance. In silico profiling predicted that while the majority maintained baseline sensitivity, the solitary A/Shymkent/NRL-1999/2024 isolate harboring the I223R substitution exhibited a multidrug-resistant genotype to oseltamivir, peramivir, and zanamivir. CONCLUSIONS: These findings demonstrate dynamic inter-seasonal etiological shifts and the predicted emergence of an independent resistant lineage (NA-I223R). This underscores the necessity for continuous, expanded genomic surveillance of respiratory pathogens across Kazakhstan. FAU - Klivleyeva, Nailya AU - Klivleyeva N AUID- ORCID: 0000-0002-4094-137X AD - The Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan. FAU - Glebova, Tatyana AU - Glebova T AUID- ORCID: 0000-0002-1139-9868 AD - The Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan. FAU - Baimukhametova, Assem AU - Baimukhametova A AUID- ORCID: 0000-0002-8959-126X AD - The Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan. FAU - Karamendin, Kobey AU - Karamendin K AUID- ORCID: 0000-0003-0829-3330 AD - The Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan. FAU - Saktaganov, Nurbol AU - Saktaganov N AUID- ORCID: 0000-0001-6526-956X AD - The Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan. FAU - Ongarbayeva, Nuray AU - Ongarbayeva N AUID- ORCID: 0000-0001-9022-331X AD - The Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan. FAU - Kalkozhayeva, Mereke AU - Kalkozhayeva M AUID- ORCID: 0000-0002-3247-2761 AD - The Research and Production Center for Microbiology and Virology, Almaty 050010, Kazakhstan. FAU - Ibragimova, Indira AU - Ibragimova I AD - Kazakhstan-Russian Medical University, Postgraduate Cardiology Course, Almaty 050000, Kazakhstan. FAU - Mutaliyeva, Aknur AU - Mutaliyeva A AUID- ORCID: 0000-0001-9608-4138 AD - National Center of Public Health Care, The Ministry of Health of the Republic of Kazakhstan, Almaty 0500008, Kazakhstan. FAU - Gabiden, Altynay AU - Gabiden A AUID- ORCID: 0000-0002-2323-5043 AD - National Center of Public Health Care, The Ministry of Health of the Republic of Kazakhstan, Almaty 0500008, Kazakhstan. FAU - Webby, Richard AU - Webby R AUID- ORCID: 0000-0002-4397-7132 AD - Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN 38105-3678, USA. LA - eng GR - AP23488011/The Science Committee of the Ministry of Science and Higher Education of the Republic of Kazakhstan/ PT - Journal Article DEP - 20261001 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (Antiviral Agents) RN - EC 3.2.1.18 (Neuraminidase) RN - L6O3XI777I (Zanamivir) RN - 20O93L6F9H (Oseltamivir) RN - 0 (Hemagglutinin Glycoproteins, Influenza Virus) RN - 0 (Acids, Carbocyclic) RN - 0 (Cyclopentanes) RN - 0 (Guanidines) RN - QW7Y7ZR15U (peramivir) RN - 0 (Viral Proteins) RN - EC 3.2.1.18 (NA protein, influenza A virus) SB - IM MH - Humans MH - Kazakhstan/epidemiology MH - *Influenza, Human/epidemiology/virology MH - Antiviral Agents/pharmacology MH - Neuraminidase/genetics MH - Phylogeny MH - *Influenza A Virus, H1N1 Subtype/genetics/drug effects/classification/isolation & purification MH - Mutation MH - Zanamivir/pharmacology MH - Oseltamivir/pharmacology MH - Seasons MH - Drug Resistance, Viral/genetics MH - Hemagglutinin Glycoproteins, Influenza Virus/genetics MH - Acids, Carbocyclic MH - Cyclopentanes/pharmacology MH - Guanidines/pharmacology MH - Viral Proteins/genetics MH - Influenza A Virus, H3N2 Subtype/genetics PMC - PMC13709524 OTO - NOTNLM OT - A(H1N1)pdm09 OT - epidemic season OT - genetic clade 6B.1A.5a.2a OT - influenza OT - mutations OT - neuraminidase inhibitors OT - oseltamivir OT - real-time PCR OT - virus OT - zanamivir COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/01 CRDT- 2026/10/27 10:46 PHST- 2026/08/13 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - v18101087 [pii] AID - viruses-18-01087 [pii] AID - 10.3390/v18101087 [doi] PST - epublish SO - Viruses. 2026 Oct 1;18(10):1087. doi: 10.3390/v18101087. PMID- 42944004 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Oct 1 TI - Integrative Machine Learning and Immunoinformatics-Guided Design and In Silico Validation of a Multiepitope DNA Vaccine Against Avian Metapneumovirus. LID - 10.3390/v18101084 [doi] LID - 1084 AB - Avian metapneumovirus (aMPV) is an emerging viral pathogen causing many outbreaks in chickens all over the world. There are several subtypes of the MPV circulating in chickens in the US. The current circulating subtypes of the virus in the US chicken are A, B, and C. Despite the availability of some aMPV vaccines in the US, most of these vaccines are based on foreign strains, particularly European aMPV-A/B strains. Their protective efficacy against contemporary U.S. aMPV isolates has not been fully established. The main goal of this study is to integrate the most recent aMPV genome sequencing data and the machine learning tools to design a novel aMPV. The machine learning tools such as epitope mapping, molecular docking, and immune simulation were used to design the multiepitope DNA vaccine based on the top-ranked epitopes of two major surface proteins of the virus (F and G). The top-ranked seventeen epitopes representing B cells, CD4 and CD8 epitopes were linked using linkers, with IL-18 added as an adjuvant. The selected epitopes showed high antigenicity, no toxicity and no allergenicity values among the screened epitopes. The molecular docking analysis of the designed vaccine construct showed a high binding affinity to the MHC class I and II epitopes to chicken alleles. The immune simulation analysis of this vaccine provides an in silico proof of concept assessment through modeling of the vaccine potential to elicit humoral and cell-mediated immunity. Further functional studies are required to test the immunogenicity and the efficacy of this novel vaccine before applications using chickens and turkey. FAU - Gazeyoglu, Fatma Nur AU - Gazeyoglu FN AUID- ORCID: 0009-0005-0070-1223 AD - Department of Veterinary Biomedical Sciences, Lewyt College of Veterinary Medicine, Long Island University, Brookville, NY 11548, USA. FAU - Shah, Abid Ullah AU - Shah AU AD - Department of Veterinary Biomedical Sciences, Lewyt College of Veterinary Medicine, Long Island University, Brookville, NY 11548, USA. FAU - Hemida, Maged Gomaa AU - Hemida MG AUID- ORCID: 0000-0003-1663-5820 AD - Department of Veterinary Biomedical Sciences, Lewyt College of Veterinary Medicine, Long Island University, Brookville, NY 11548, USA. LA - eng GR - NI26AHDRXXXXG063/United States Department of Agriculture/ PT - Journal Article DEP - 20261001 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (Vaccines, DNA) RN - 0 (Viral Vaccines) RN - 0 (Epitopes) RN - 0 (Epitopes, T-Lymphocyte) SB - IM MH - Animals MH - *Metapneumovirus/immunology/genetics MH - Immunoinformatics MH - *Machine Learning MH - *Vaccines, DNA/immunology/genetics MH - *Viral Vaccines/immunology/genetics MH - Chickens MH - Molecular Docking Simulation MH - *Poultry Diseases/prevention & control/virology/immunology MH - *Paramyxoviridae Infections/veterinary/prevention & control/immunology/virology MH - Computer Simulation MH - Epitope Mapping MH - *Epitopes/immunology/genetics MH - Epitopes, T-Lymphocyte/immunology PMC - PMC13709672 OTO - NOTNLM OT - DNA vaccine OT - aMPV OT - avian metapneumovirus OT - epitope mapping OT - in silico prediction OT - machine learning OT - molecular docking COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/01 CRDT- 2026/10/27 10:46 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - v18101084 [pii] AID - viruses-18-01084 [pii] AID - 10.3390/v18101084 [doi] PST - epublish SO - Viruses. 2026 Oct 1;18(10):1084. doi: 10.3390/v18101084. PMID- 42944000 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Sep 30 TI - HPV6/11 Persistence and Treatment-Associated Immune Remodeling in Recurrent Respiratory Papillomatosis: A Cross-Cohort Transcriptomic Analysis. LID - 10.3390/v18101080 [doi] LID - 1080 AB - Recurrent respiratory papillomatosis (RRP), caused mainly by human papillomavirus types 6 and 11 (HPV6/11), can persist despite the absence of recognized systemic immunodeficiency. We reanalyzed three transcriptomic datasets using the same predefined immune-gene programs: paired papilloma and autologous laryngeal mucosa from 12 patients, single-cell RNA sequencing from papillomas of four RRP donors and pharyngopalatine mucosa from three controls, and paired papilloma samples from 17 patients before and after INO-3107, a DNA immunotherapy encoding HPV6/11 antigens and interleukin-12. In untreated tissue, cluster of differentiation 8 (CD8)- and natural killer (NK)-associated cytotoxic program scores were lower and the transforming growth factor beta (TGF-beta) suppressive program score was higher, reproducing findings from the same cohort. The lower cytotoxic pattern was not reproduced within the recovered combined T-cell and NK-cell population, although no single-cell comparison remained significant after correction for multiple testing. After INO-3107, CD8- and NK-associated cytotoxic, T helper 1 (Th1)/interferon gamma (IFN-gamma), and C-X-C motif chemokine receptor 3 (CXCR3)-associated trafficking program scores increased significantly, whereas TGF-beta and M2-like suppressive programs showed no comparable statistically supported change. Together, these findings show that different components of the local immune response do not change in parallel and support a model of selective treatment-associated immune remodeling. This pattern may inform future biomarker and combination-treatment studies. FAU - Jedruchniewicz, Natalia AU - Jedruchniewicz N AUID- ORCID: 0000-0002-6515-5762 AD - Research and Development Center, Regional Specialist Hospital in Wroclaw, 51-124 Wroclaw, Poland. FAU - Klimek, Tomasz AU - Klimek T AUID- ORCID: 0009-0006-1283-8187 AD - Research and Development Center, Regional Specialist Hospital in Wroclaw, 51-124 Wroclaw, Poland. AD - Laboratory of Phage Molecular Biology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland. FAU - Klimza, Hanna AU - Klimza H AUID- ORCID: 0000-0003-1652-9040 AD - Research and Development Center, Regional Specialist Hospital in Wroclaw, 51-124 Wroclaw, Poland. FAU - Dabrowska, Krystyna AU - Dabrowska K AUID- ORCID: 0000-0002-1518-3183 AD - Laboratory of Phage Molecular Biology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland. AD - Faculty of Medicine, Wroclaw University of Science and Technology, 50-370 Wroclaw, Poland. FAU - Wierzbicka, Malgorzata AU - Wierzbicka M AUID- ORCID: 0000-0003-0006-6352 AD - Research and Development Center, Regional Specialist Hospital in Wroclaw, 51-124 Wroclaw, Poland. AD - Faculty of Medicine, Wroclaw University of Science and Technology, 50-370 Wroclaw, Poland. AD - Institute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland. LA - eng GR - 2023/51/B/NZ7/00799/National Science Centre/ PT - Journal Article DEP - 20260930 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - Recurrent respiratory papillomatosis SB - IM MH - Humans MH - *Papillomavirus Infections/immunology/virology/therapy MH - *Respiratory Tract Infections/immunology/virology/therapy/drug therapy MH - *Human papillomavirus 11/immunology/genetics MH - *Human papillomavirus 6/immunology/genetics MH - Gene Expression Profiling MH - Female MH - Transcriptome MH - Killer Cells, Natural/immunology MH - Cohort Studies MH - Male PMC - PMC13709832 OTO - NOTNLM OT - INO-3107 OT - TGF-beta OT - cellular immunity OT - human papillomavirus 11 OT - human papillomavirus 6 OT - recurrent respiratory papillomatosis OT - single-cell RNA sequencing OT - transcriptomics OT - viral persistence COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/09/30 CRDT- 2026/10/27 10:46 PHST- 2026/08/18 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - v18101080 [pii] AID - viruses-18-01080 [pii] AID - 10.3390/v18101080 [doi] PST - epublish SO - Viruses. 2026 Sep 30;18(10):1080. doi: 10.3390/v18101080. PMID- 42943988 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Sep 26 TI - Coronavirus Exploitation of Ubiquitination and Selective Autophagy to Control Innate Immunity: Mechanisms, Systems Logic, and Therapeutic Opportunities. LID - 10.3390/v18101068 [doi] LID - 1068 AB - Coronaviruses replicate in membrane-rich cytoplasmic niches while confronting innate immune systems whose activation, signal propagation, and termination are extensively controlled by ubiquitin and autophagy. These two regulatory systems are often discussed separately. Yet they form a coupled information-and-disposal network: ubiquitin chains encode the identity and fate of immune proteins or viral cargo, whereas selective autophagy receptors read these signals and route substrates to lysosomes. Coronaviruses exploit this coupling at several levels. Papain-like proteases remove ubiquitin and ISG15 from antiviral factors; structural, nonstructural, and accessory proteins redirect host ubiquitin ligases or destabilize immune adaptors; and viral proteins remodel autophagosome biogenesis, mitophagy, lysosome function, and selective virophagy. These activities do more than globally suppress immunity. They delay early interferon production, reshape inflammatory signaling, preserve replication organelles, adjust mitochondrial quality control, and protect viral proteins from cargo-selective degradation. Importantly, the outcome is context dependent. Autophagy initiation can support membrane supply or suppress mitochondrial antiviral signaling, whereas completion of autophagic flux can eliminate viral material and restrain damaging inflammation. Likewise, K48-linked ubiquitination may remove antiviral proteins, but nondegradative K63- or M1-linked chains can either assemble antiviral signalosomes or promote pathological NF-kappaB activity. Here, we synthesize evidence from human and animal coronaviruses to propose that viral immune evasion is governed by spatially and temporally selective rewiring rather than indiscriminate inhibition. We distinguish mechanisms demonstrated during infection from those inferred from ectopic expression, identify points of convergence among RIG-I-like receptor, cGAS-STING, NF-kappaB, inflammasome, and autophagy pathways, and discuss why apparently contradictory observations often reflect differences in flux, cell type, infection stage, and viral lineage. This integrated view highlights viral proteases, host E3 ligase-substrate interfaces, autophagy receptors, and organelle contact sites as therapeutic nodes, while emphasizing that interventions must preserve the homeostatic functions of ubiquitin and autophagy. FAU - Wang, Pan AU - Wang P AD - Institute of Animal Science, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan 750002, China. AD - School of Animal Science and Technology, Ningxia University, Yinchuan 750021, China. FAU - Zhao, Yuxi AU - Zhao Y AUID- ORCID: 0000-0002-2080-3412 AD - Institute of Animal Science, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan 750002, China. FAU - Yang, Yuqiu AU - Yang Y AD - School of Animal Science and Technology, Ningxia University, Yinchuan 750021, China. FAU - Zhang, Juan AU - Zhang J AD - School of Animal Science and Technology, Ningxia University, Yinchuan 750021, China. FAU - Wu, Junfang AU - Wu J AD - School of Animal Science and Technology, Ningxia University, Yinchuan 750021, China. FAU - Wang, Jiandong AU - Wang J AUID- ORCID: 0000-0002-9207-5100 AD - Institute of Animal Science, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan 750002, China. LA - eng PT - Journal Article PT - Review DEP - 20260926 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (Ubiquitin) RN - 0 (Viral Proteins) SB - IM MH - Humans MH - *Immunity, Innate MH - *Autophagy/immunology MH - *Ubiquitination MH - Animals MH - *Coronavirus/immunology/physiology MH - Signal Transduction MH - Host-Pathogen Interactions/immunology MH - *Coronavirus Infections/immunology/virology MH - Mitophagy MH - Ubiquitin/metabolism MH - Viral Proteins/metabolism PMC - PMC13709353 OTO - NOTNLM OT - ISGylation OT - SARS-CoV-2 OT - coronavirus OT - deubiquitination OT - innate immunity OT - interferon OT - mitophagy OT - selective autophagy OT - ubiquitin OT - virophagy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/09/26 CRDT- 2026/10/27 10:46 PHST- 2026/08/20 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/26 00:00 [pmc-release] AID - v18101068 [pii] AID - viruses-18-01068 [pii] AID - 10.3390/v18101068 [doi] PST - epublish SO - Viruses. 2026 Sep 26;18(10):1068. doi: 10.3390/v18101068. PMID- 42943987 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Sep 26 TI - Evolutionary Dynamics of Rift Valley Fever Virus in Saudi Arabia: Whole-Genome Analysis of the 2000-2001 Outbreak Strains. LID - 10.3390/v18101067 [doi] LID - 1067 AB - Rift Valley fever virus (RVFV) is a mosquito-borne zoonotic pathogen that causes severe disease in livestock and humans, with epidemic potential and a capacity for geographic expansion beyond its African endemic range. The 2000-2001 outbreak in Saudi Arabia represented the first confirmed introduction of RVFV outside Africa, yet comprehensive multi-segment genomic characterization of the circulating strains has remained incomplete. Here, we performed an integrated evolutionary and molecular analysis of the three complete-genome Saudi RVFV strains (Saudi 2000-10911, Saudi 200010901, and SA01-1322) using publicly available sequences. Whole-genome comparisons revealed substantial nucleotide and amino acid diversity, with the L segment exhibiting the highest mutation burden (182 nucleotide substitutions, 17 amino acid changes), while the NSs region of the S segment accumulated 52 nucleotide and 11 amino acid mutations compared to the highly conserved nucleocapsid domain. Maximum-likelihood phylogenetic analyses of all three segments consistently placed the Saudi strains within Lineage C (bootstrap = 100/100), clustering with East African strains from Kenya (2006-2007), Tanzania (2007), and South Africa (2008-2010), with congruent topologies across segments confirming the absence of reassortment. Bayesian phylogeographic analysis of 118 L-segment sequences estimated a mean substitution rate of 1.697 x 10(-4) substitutions/site/year (95% HPD: 1.5-1.9 x 10(-4)) and a time to the most recent common ancestor of approximately 1857 (95% HPD: 1835-1876). Selection-pressure analyses using four complementary methods (MEME, FEL, FUBAR, and SLAC) demonstrated pervasive purifying selection across all three segments, with only sporadic episodic positive selection detected at a limited number of codons (six in L, seven in M, and one in S). In silico glycosylation prediction identified conserved N- and O-glycosylation sites on the M segment, with the Saudi strains displaying a Lineage C-typical profile lacking geographically restricted markers. However, the absence of canonical signal peptides in all sequences warrants cautious interpretation of these predictions. Collectively, our findings are consistent with a single introduction of Lineage C RVFV from East Africa among the sampled strains, with no subsequent reassortment, and that the virus has evolved under strong functional constraints with only rare, lineage-restricted episodes of positive selection. The identified Saudi-specific and lineage-defining mutations provide molecular markers for surveillance, while the high conservation of core proteins supports the feasibility of broadly protective vaccines. FAU - Farrag, Mohamed A AU - Farrag MA AUID- ORCID: 0000-0002-5632-6750 AD - Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. FAU - Aljowaie, Reem M AU - Aljowaie RM AUID- ORCID: 0000-0001-5337-5381 AD - Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. FAU - Aziz, Ibrahim M AU - Aziz IM AUID- ORCID: 0000-0002-6970-5207 AD - Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. FAU - Almosa, Abdulaziz Abdullah AU - Almosa AA AD - Wellness Prevention Medicine, King Abdulaziz City for Science and Technology (KACST), Riyadh 11451, Saudi Arabia. FAU - Alnafjan, Basel Mohammed AU - Alnafjan BM AUID- ORCID: 0009-0002-2055-7335 AD - Wellness Prevention Medicine, King Abdulaziz City for Science and Technology (KACST), Riyadh 11451, Saudi Arabia. FAU - Marraiki, Najat A Y AU - Marraiki NAY AD - Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. LA - eng GR - Ongoing Research Funding Program (ORF-2026-1743), King Saud University, Riyadh, Saudi Arabia/King Saud University/ PT - Journal Article DEP - 20260926 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 SB - IM MH - Saudi Arabia/epidemiology MH - *Rift Valley fever virus/genetics/classification/isolation & purification MH - *Rift Valley Fever/epidemiology/virology MH - *Evolution, Molecular MH - Phylogeny MH - *Genome, Viral MH - *Disease Outbreaks MH - Animals MH - Humans MH - Whole Genome Sequencing MH - Genetic Variation MH - Mutation PMC - PMC13709933 OTO - NOTNLM OT - Rift Valley fever virus OT - Saudi Arabia OT - glycosylation OT - lineage C OT - molecular dating OT - phylogenomics OT - reassortment OT - selection pressure COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/09/26 CRDT- 2026/10/27 10:46 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/26 00:00 [pmc-release] AID - v18101067 [pii] AID - viruses-18-01067 [pii] AID - 10.3390/v18101067 [doi] PST - epublish SO - Viruses. 2026 Sep 26;18(10):1067. doi: 10.3390/v18101067. PMID- 42943986 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Sep 25 TI - Protein-Protein Interaction-Guided Peptides Interfere with Early Zika Virus Entry-Associated Events. LID - 10.3390/v18101066 [doi] LID - 1066 AB - Zika virus (ZIKV) remains a public health concern because of its association with neurological complications and congenital disease, yet no specific antiviral therapy has been approved. Since the envelope (E) protein mediates viral attachment and early entry-associated membrane events, it represents an attractive antiviral target. Here, we used a protein-protein interaction-guided strategy to design peptides to interfere with predicted interfaces between the ZIKV E protein and cellular receptors involved in attachment. After in silico filtering and preliminary screening, six peptides (PZ1-PZ6) were selected. These peptides showed heterogeneous physicochemical profiles, low cytotoxicity and hemolytic activity under the tested conditions, and docking-predicted interactions with functional E protein regions. In HTR-8/SVneo cells, antiviral activity depended on the timing of exposure, with greater reductions in infectious titers during co-treatment and post-treatment. All peptides also reduced titers in anti-adsorption assays, supporting interference with viral attachment or early entry-associated events. Consistently, DiOC18 fluorescence signals were reduced in a peptide-specific manner, particularly for PZ3 and PZ6. Nuclease protection assays showed no extensive exposure of the viral genome, whereas transmission electron microscopy revealed ultrastructural alterations without generalized particle disruption. Overall, PZ1-PZ6 are promising protein-protein interaction-guided anti-ZIKV peptide candidates with activity against early viral entry-associated events. FAU - Rodriguez-Salazar, Carlos Andres AU - Rodriguez-Salazar CA AUID- ORCID: 0000-0002-0071-1289 AD - Molecular Biology and Virology Laboratory, Faculty of Medicine and Health Sciences, Corporacion Universitaria Empresarial Alexander von Humboldt, Armenia 630003, Quindio, Colombia. FAU - Recalde-Reyes, Delia Piedad AU - Recalde-Reyes DP AUID- ORCID: 0000-0002-7752-1221 AD - Molecular Biology and Virology Laboratory, Faculty of Medicine and Health Sciences, Corporacion Universitaria Empresarial Alexander von Humboldt, Armenia 630003, Quindio, Colombia. FAU - Linares-Jimenez, Santiago AU - Linares-Jimenez S AD - Molecular Biology and Virology Laboratory, Faculty of Medicine and Health Sciences, Corporacion Universitaria Empresarial Alexander von Humboldt, Armenia 630003, Quindio, Colombia. FAU - Lopez-Cardoso, Carolina AU - Lopez-Cardoso C AD - Molecular Biology and Virology Laboratory, Faculty of Medicine and Health Sciences, Corporacion Universitaria Empresarial Alexander von Humboldt, Armenia 630003, Quindio, Colombia. FAU - Giraldo, Maria Isabel AU - Giraldo MI AD - Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA. LA - eng GR - K12 HD052023/HD/NICHD NIH HHS/United States GR - K12HD052023/National Institutes of Health (NIH), Office of the Director / BIRCWH Program/ GR - No. D.I.M.02-2023/Corporacion Universitaria Empresarial Alexander von Humboldt/ PT - Journal Article DEP - 20260925 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (Antiviral Agents) RN - 0 (Peptides) RN - 0 (Viral Envelope Proteins) SB - IM MH - *Zika Virus/drug effects/physiology MH - *Virus Internalization/drug effects MH - Humans MH - *Antiviral Agents/pharmacology/chemistry MH - *Peptides/pharmacology/chemistry MH - *Viral Envelope Proteins/metabolism/chemistry MH - Cell Line MH - Animals MH - Zika Virus Infection/virology/drug therapy MH - Protein Binding MH - Virus Attachment/drug effects PMC - PMC13709921 OTO - NOTNLM OT - Zika virus OT - antimicrobial peptides OT - antiviral agents OT - computational biology OT - viral fusion proteins OT - virus internalization COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/09/25 CRDT- 2026/10/27 10:46 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - v18101066 [pii] AID - viruses-18-01066 [pii] AID - 10.3390/v18101066 [doi] PST - epublish SO - Viruses. 2026 Sep 25;18(10):1066. doi: 10.3390/v18101066. PMID- 42943977 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Sep 24 TI - HTLV Seroprevalence and Associated Factors Among Blood Donors in Shenzhen, China: A Cross-Sectional Study with Longitudinal Follow-Up, 2013-2022. LID - 10.3390/v18101058 [doi] LID - 1058 AB - BACKGROUND: Blood transfusion is a major route of Human T-lymphotropic virus (HTLV) transmission. In this study, we conducted a cross-sectional study of HTLV seroprevalence among blood donors in Shenzhen, with secondary investigations covering signal-to-cutoff (S/CO) predictive value, longitudinal follow-up, epidemiological characteristics, and possible familial clustering. METHODS: Retrospective analysis was conducted on 512,200 Shenzhen Blood Center donors (2014-2022, with partial 2013 data). ELISA was utilized for initial HTLV screening, with MP, INNO-LIA and qPCR for confirmation. Confirmed cases received questionnaire follow-up and retesting. Some relatives of confirmed cases also received HTLV-1/2 testing. RESULTS: Among 512,200 donors, 203 were screening-reactive, with 18 confirmed-positive cases (3.51 per 100,000 donors; 95% CI: 2.08-5.55) and 10 indeterminate cases. The screening-reactive rate declined significantly over the decade (p < 0.001), whereas the confirmed-positive rate remained low and showed no significant temporal trend (p = 0.363). Among initially screening-reactive donors, high S/CO values strongly predicted confirmatory positivity, whereas low-level reactivity was mostly transient. Questionnaire data were available for 9 of the 18 confirmed-positive donors (50.0%) and 51 control donors. In this exploratory analysis, only invasive medical procedures occurring in Fujian remained significantly associated with infection (adjusted p = 0.010). Two of the 10 tested family members were confirmed-positive, suggesting possible familial clustering. CONCLUSIONS: The confirmed prevalence of HTLV among Shenzhen blood donors was extremely low, and screening reactivity declined significantly over the ten-year period. Screening S/CO values strongly predicted confirmatory results. Fujian-related invasive medical procedures and possible familial clustering are exploratory findings that require confirmation in larger studies. Continued donor screening and blood safety surveillance remain warranted. FAU - Xu, Xiaoxuan AU - Xu X AD - Department of Laboratory, Shenzhen Blood Center, Shenzhen 518035, China. FAU - Wang, Dian AU - Wang D AD - Department of Microbiology and Infectious Disease Research Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China. FAU - Guan, Guiwen AU - Guan G AD - College of Veterinary Medicine, Northwest A&F University, Yangling, Xianyang 712199, China. FAU - Wei, Guochao AU - Wei G AUID- ORCID: 0000-0002-7768-2404 AD - Department of Microbiology and Infectious Disease Research Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China. FAU - Wu, Linfeng AU - Wu L AD - Department of Laboratory, Shenzhen Blood Center, Shenzhen 518035, China. FAU - Liu, Heng AU - Liu H AD - Department of Laboratory, Shenzhen Blood Center, Shenzhen 518035, China. FAU - Li, Ran AU - Li R AD - Department of Laboratory, Shenzhen Blood Center, Shenzhen 518035, China. FAU - Wang, Haobiao AU - Wang H AD - Department of Laboratory, Shenzhen Blood Center, Shenzhen 518035, China. FAU - Chen, Tingting AU - Chen T AD - Department of Laboratory, Shenzhen Blood Center, Shenzhen 518035, China. FAU - Lu, Fengmin AU - Lu F AD - Department of Microbiology and Infectious Disease Research Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China. FAU - Xue, Yuanye AU - Xue Y AUID- ORCID: 0000-0002-3813-5934 AD - Department of Laboratory, Shenzhen Blood Center, Shenzhen 518035, China. FAU - Chen, Xiangmei AU - Chen X AD - Department of Microbiology and Infectious Disease Research Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China. FAU - Zeng, Jinfeng AU - Zeng J AD - Department of Laboratory, Shenzhen Blood Center, Shenzhen 518035, China. LA - eng GR - SZSM202311032/Sanming Project of Medicine in Shenzhen/ GR - SZXK070/Shenzhen Key Discipline Project of Blood Transfusion Medicine/ GR - SZBC202303/Research Project of Shenzhen Blood Center/ GR - SZBC202408/Research Project of Shenzhen Blood Center/ GR - SZBC202514/Research Project of Shenzhen Blood Center/ PT - Journal Article DEP - 20260924 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 SB - IM MH - Humans MH - *Blood Donors/statistics & numerical data MH - Seroepidemiologic Studies MH - Cross-Sectional Studies MH - Female MH - China/epidemiology MH - Male MH - Adult MH - Follow-Up Studies MH - Middle Aged MH - Longitudinal Studies MH - Retrospective Studies MH - Human T-lymphotropic virus 2/immunology MH - *HTLV-I Infections/epidemiology MH - *Human T-lymphotropic virus 1/immunology MH - Blood Donation MH - Young Adult MH - *HTLV-II Infections/epidemiology PMC - PMC13709397 OTO - NOTNLM OT - HTLV OT - blood donors OT - blood safety OT - epidemiological study OT - follow-up OT - seroprevalence COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/09/24 CRDT- 2026/10/27 10:46 PHST- 2026/07/23 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - v18101058 [pii] AID - viruses-18-01058 [pii] AID - 10.3390/v18101058 [doi] PST - epublish SO - Viruses. 2026 Sep 24;18(10):1058. doi: 10.3390/v18101058. PMID- 42943976 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4915 (Electronic) IS - 1999-4915 (Linking) VI - 18 IP - 10 DP - 2026 Sep 23 TI - Antiviral Sesquiterpene Lactones from Laurus nobilis L.: Inhibition of Hepatitis C Virus and Dengue Virus Replication. LID - 10.3390/v18101056 [doi] LID - 1056 AB - Natural products remain indispensable scaffolds in antiviral drug discovery, particularly against high-burden viral pathogens. In this study, two major sesquiterpene lactones, dehydrocostus lactone (1) and costunolide (2), were isolated from Laurus nobilis L. leaves and evaluated for their antiviral potential against hepatitis C virus (HCV) and dengue virus (DENV). The structures of both compounds were confirmed by HRMS and 1D NMR spectroscopic analyses. In subgenomic replicon assays, the molecules inhibited HCV replication in the low-micromolar range (EC50=2.34 muM for 1 and 2.06 muM for 2) with favorable selectivity indices. In full-length HCV reporter systems, costunolide (2) displayed enhanced anti-HCV potency, reduced viral RNA and NS5A protein levels, and enhanced the suppressive effect of the clinically approved NS5A inhibitor, daclatasvir. Furthermore, costunolide (2) exhibited cross-flaviviridae activity, suppressing DENV replication (EC50=5.89 muM in replicon cells; EC50=1.27 muM post-infection in infectious virus assays), and demonstrated cooperative suppression of viral propagation along with reduced glutathione. Mechanistically, these results highlight the critical role of the sesquiterpene lactone core, and the observed enhanced combined activity between costunolide and reduced glutathione is consistent with a possible contribution of the NRF2/glutathione antioxidant axis to its anti-DENV action. Overall, this study highlights L. nobilis as a previously underexplored source of antiviral sesquiterpene lactones and establishes costunolide (2) as a promising lead against HCV and DENV, warranting further investigation across additional Flaviviridae members. FAU - Amen, Yhiya AU - Amen Y AUID- ORCID: 0000-0003-2552-4567 AD - Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt. AD - Department of Agro-Environmental Sciences, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 819-0395, Japan. FAU - Elsbaey, Marwa AU - Elsbaey M AUID- ORCID: 0000-0003-2044-2917 AD - Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt. FAU - Mpekoulis, George AU - Mpekoulis G AD - Molecular Virology, Hellenic Pasteur Institute, 11521 Athens, Greece. FAU - Christou, Evangelia AU - Christou E AUID- ORCID: 0000-0001-9234-481X AD - Molecular Virology, Hellenic Pasteur Institute, 11521 Athens, Greece. FAU - Tsimpili, Helena AU - Tsimpili H AUID- ORCID: 0009-0009-4560-4610 AD - Division of Pharmaceutical Chemistry, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Panepistimiopolis-Zografou, 15771 Athens, Greece. FAU - Vassilaki, Niki AU - Vassilaki N AUID- ORCID: 0000-0001-7992-3438 AD - Molecular Virology, Hellenic Pasteur Institute, 11521 Athens, Greece. FAU - Shimizu, Kuniyoshi AU - Shimizu K AUID- ORCID: 0000-0001-5960-1503 AD - Department of Agro-Environmental Sciences, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka 819-0395, Japan. AD - Institute of Asian and Oceanic Studies, Kyushu University, Fukuoka 819-0395, Japan. FAU - Zoidis, Grigoris AU - Zoidis G AUID- ORCID: 0000-0002-9442-5186 AD - Division of Pharmaceutical Chemistry, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Panepistimiopolis-Zografou, 15771 Athens, Greece. LA - eng PT - Journal Article DEP - 20260923 PL - Switzerland TA - Viruses JT - Viruses JID - 101509722 RN - 0 (Antiviral Agents) RN - 0 (Sesquiterpenes) RN - 0 (Lactones) RN - 4IK578SA7Z (costunolide) RN - 477-43-0 (dehydrocostus lactone) RN - 0 (Plant Extracts) SB - IM MH - *Antiviral Agents/pharmacology/chemistry/isolation & purification MH - *Virus Replication/drug effects MH - *Dengue Virus/drug effects/physiology MH - *Sesquiterpenes/pharmacology/chemistry/isolation & purification MH - *Lactones/pharmacology/chemistry/isolation & purification MH - *Hepacivirus/drug effects/physiology MH - Humans MH - *Laurus/chemistry MH - Cell Line MH - Plant Extracts/pharmacology/chemistry PMC - PMC13709689 OTO - NOTNLM OT - Laurus nobilis OT - antiviral activity OT - costunolide OT - dehydrocostus lactone OT - dengue virus OT - hepatitis C virus OT - natural products OT - sesquiterpene lactones COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/09/23 CRDT- 2026/10/27 10:46 PHST- 2026/08/19 00:00 [received] PHST- 2026/09/09 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - v18101056 [pii] AID - viruses-18-01056 [pii] AID - 10.3390/v18101056 [doi] PST - epublish SO - Viruses. 2026 Sep 23;18(10):1056. doi: 10.3390/v18101056. PMID- 42943967 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Oct 21 TI - Wearable Soft Sensors for Spinal Monitoring: Soft Materials, Multimodal Integration, and Clinical Translation. LID - 10.3390/mi17101210 [doi] LID - 1210 AB - Spinal musculoskeletal disorders demand continuous assessment of movement and loading, whereas routine clinical imaging provides important but episodic structural information. While wearable soft sensors offer a non-invasive approach to complement these assessments, translating laboratory-scale prototypes into robust clinical systems remains challenging because of electromechanical stability, signal fidelity, and data interpretation. This review critically examines the end-to-end development of soft wearable technologies for spinal monitoring, moving beyond a materials-focused survey to address these translational gaps. First, we evaluate advances in soft sensing mechanisms, highlighting the trade-offs between mechanical compliance and stable sensor performance. We then examine system-level integration, including soft or flexible sensing elements, conventional sensors on compliant substrates, rigid-soft interfaces, and multimodal systems for monitoring posture, motion, pressure, and muscle activity. Furthermore, we discuss signal-processing and artificial intelligence-assisted workflows for denoising and extracting meaningful physiological features. By comparing reported performance metrics and validation settings, we assess representative applications in scoliosis and chronic low back pain. Current evidence demonstrates technical feasibility in laboratory tests and small studies, but does not yet establish broad diagnostic benefit or routine clinical effectiveness. Finally, we identify key evidence gaps, including external validation, patient-based studies, standardized protocols, and long-term assessment, that must be addressed for clinical translation. FAU - Li, Chengyu AU - Li C AUID- ORCID: 0000-0002-4434-0838 AD - School of Fashion and Textiles, the Hong Kong Polytechnic University, Hong Kong 999077, China. AD - Beijing WeiNa ZhiLian Technology Co., Ltd., Beijing 101101, China. FAU - Xu, Shuxing AU - Xu S AUID- ORCID: 0000-0003-0748-5867 AD - Beijing WeiNa ZhiLian Technology Co., Ltd., Beijing 101101, China. AD - School of Nanoscience and Engineering, University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Li, Wenbo AU - Li W AD - School of Fashion and Textiles, the Hong Kong Polytechnic University, Hong Kong 999077, China. FAU - Jin, Dian AU - Jin D AD - School of Fashion and Textiles, the Hong Kong Polytechnic University, Hong Kong 999077, China. FAU - Zhao, Yudong AU - Zhao Y AD - Department of Orthopaedics, Peking University Third Hospital, Beijing 100191, China. AD - Engineering Research Center of Bone and Joint Precision Medicine, Ministry of Education, Beijing 100191, China. FAU - Zhang, Chao AU - Zhang C AD - School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China. FAU - Li, Weishi AU - Li W AUID- ORCID: 0000-0001-9512-5436 AD - Department of Orthopaedics, Peking University Third Hospital, Beijing 100191, China. AD - Engineering Research Center of Bone and Joint Precision Medicine, Ministry of Education, Beijing 100191, China. FAU - Sun, Zhuoran AU - Sun Z AD - Department of Orthopaedics, Peking University Third Hospital, Beijing 100191, China. AD - Engineering Research Center of Bone and Joint Precision Medicine, Ministry of Education, Beijing 100191, China. FAU - Zhou, Siyu AU - Zhou S AUID- ORCID: 0000-0002-2427-6242 AD - Department of Orthopaedics, Peking University Third Hospital, Beijing 100191, China. AD - Engineering Research Center of Bone and Joint Precision Medicine, Ministry of Education, Beijing 100191, China. LA - eng GR - 82302739/National Natural Science Foundation of China/ GR - 2023YFC3604400/National Key Research and Development Program of China/ GR - BYSYZD2023048/Key Clinical Projects of the hospital/ PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707731 OTO - NOTNLM OT - clinical applications OT - flexible materials OT - signal processing OT - soft sensors OT - spinal monitoring COIS- Author Chengyu Li and Shuxing Xu were employed by Beijing WeiNa ZhiLian Technology Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:46 PHST- 2026/08/24 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - mi17101210 [pii] AID - micromachines-17-01210 [pii] AID - 10.3390/mi17101210 [doi] PST - epublish SO - Micromachines (Basel). 2026 Oct 21;17(10):1210. doi: 10.3390/mi17101210. PMID- 42943950 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Oct 15 TI - Deep-Learning-Assisted Integrated Microfluidic Platform for Automated Analysis of EV RNA Mutations. LID - 10.3390/mi17101193 [doi] LID - 1193 AB - Extracellular vesicles (EVs) carry key genetic biomarkers, such as somatic mutations, making them valuable targets for non-invasive cancer diagnostics. However, standard workflows that isolate EVs and detect low-abundance mutations typically require multi-step manual operations, leading to sample loss and potential cross-contamination. Here, we present an integrated microfluidic platform combined with a deep learning algorithm for the continuous processing and automated droplet analysis of EV-derived RNA mutations. The microfluidic chip integrates magnetic bead-based EV capture, online lysis, droplet generation, and arrayed RT-droplet digital PCR (RT-ddPCR) on a single device. To streamline signal readout, a deep learning framework based on Cellpose and K-means clustering was implemented to process individual fluorescence images, enabling droplet detection, background removal, gray-scale intensity extraction, and automatic positive/negative droplet classification. The system showed a linear response across a wide range of original-sample inputs (R(2) = 0.9950). The platform was evaluated using EV samples derived from three cancer cell lines (NCI-H1975, SK-LU-1, and HepG2) and the detection of EGFR L858R and KRAS mutations were achieved. By coupling continuous sample processing with automated image interpretation, this platform offers a practical reference for integrated EV nucleic acid analysis for liquid-biopsy applications. FAU - Lu, Yunxing AU - Lu Y AD - School of Science and Technology, Shanghai Open University, Shanghai 200433, China. FAU - Wang, Xiaohan AU - Wang X AD - The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China. AD - Shanghai Key Laboratory of Embryo Original Diseases, Shanghai 200030, China. FAU - Bai, Qingchun AU - Bai Q AD - Shanghai Engineering Research Center of Open Distance Education, Shanghai Open University, Shanghai 200433, China. FAU - Shi, Qiang AU - Shi Q AUID- ORCID: 0000-0003-4089-5892 AD - School of Science and Technology, Shanghai Open University, Shanghai 200433, China. FAU - Song, Yiman AU - Song Y AD - State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China. AD - School of Stomatology, Dalian Medical University, Dalian 116044, China. FAU - Zhou, Lin AU - Zhou L AD - State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China. FAU - Wu, Zhenhua AU - Wu Z AD - State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China. FAU - Mao, Hongju AU - Mao H AD - Center for Medical Research and Innovation, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai 200120, China. LA - eng GR - No.62601528/National Natural Science Foundation of China/ GR - No. 23CGB01/the Chenguang Program of Shanghai Education Development Foundation and Shanghai Municipal Education Commission/ GR - 62231025/National Natural Science Foundation of China/ GR - 24XTCX00700/Science and Technology Commission of Shanghai Municipality/ GR - 20DZ2254400/Shanghai Engineer & Technology Research Center of Internet of Things for Respiratory Medicine/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707912 OTO - NOTNLM OT - RNA mutation OT - deep learning OT - droplet digital PCR OT - exosome OT - extracellular vesicle OT - liquid biopsy OT - microfluidics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:46 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - mi17101193 [pii] AID - micromachines-17-01193 [pii] AID - 10.3390/mi17101193 [doi] PST - epublish SO - Micromachines (Basel). 2026 Oct 15;17(10):1193. doi: 10.3390/mi17101193. PMID- 42943947 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Oct 14 TI - Geometry Transfer Using Cross Sections for Sequential Mechanical-Electrostatic Simulation of Microstructured Flexible Capacitive Pressure Sensors. LID - 10.3390/mi17101190 [doi] LID - 1190 AB - Predicting how microstructure dimensions affect the response of flexible capacitive pressure sensors can guide sensor design. Large deformation and contact, however, complicate the construction of dielectric and air domains from the deformed mechanical geometry. This study presents a geometry transfer method based on equivalent cross sections. The method uses the finite element software packages Abaqus 2023 and COMSOL Multiphysics 6.2 for mechanical and electrostatic analysis, respectively. The electrostatic domains are reconstructed from horizontal section areas of the deformed boundary, current polydimethylsiloxane layer thicknesses and microstructure height. The method was evaluated using two square frustum geometries and measurements from three independent devices. Five equivalent square sections yielded volume meshes and capacitance solutions for all 29 selected states of each geometry. For the baseline geometry, capacitance differed from a 17-section reference by at most 0.1092% across four states spanning 0-411.85 kPa. Replacing equivalent squares with fitted contours of equal area changed capacitance by at most 0.04835%. A microscopy-guided geometry adjustment preserved the initial electrode separation and sidewall angle. Over 0-350 kPa, the adjustment reduced the root mean square error between the predicted relative capacitance response and the experimental mean from 0.0721 to 0.0292. The method provides a practical route from nonlinear deformation to capacitance prediction for microstructure design. FAU - Shao, Yiwei AU - Shao Y AUID- ORCID: 0000-0002-0693-3042 AD - State Key Laboratory for Manufacturing Systems Engineering, School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an 710049, China. FAU - Bi, Wenzhe AU - Bi W AD - State Key Laboratory for Manufacturing Systems Engineering, School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an 710049, China. FAU - Zhang, Qi AU - Zhang Q AUID- ORCID: 0000-0003-1611-8460 AD - State Key Laboratory for Manufacturing Systems Engineering, School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an 710049, China. FAU - Zhang, Xinyu AU - Zhang X AD - State Key Laboratory for Manufacturing Systems Engineering, School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an 710049, China. FAU - Liang, Xiaoya AU - Liang X AD - State Key Laboratory for Manufacturing Systems Engineering, School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an 710049, China. FAU - Xiao, Kangping AU - Xiao K AD - State Key Laboratory for Manufacturing Systems Engineering, School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an 710049, China. FAU - Zhao, Yulong AU - Zhao Y AD - State Key Laboratory for Manufacturing Systems Engineering, School of Mechanical Engineering, Xi'an Jiaotong University, Xi'an 710049, China. LA - eng GR - 2022KXJ-134/Qinchuangyuan "Scientist + Engineer" Team Development Program/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707780 OTO - NOTNLM OT - capacitive pressure sensor OT - cross-sectional area OT - finite element analysis OT - geometry transfer OT - sequential mechanical-electrostatic simulation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:46 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - mi17101190 [pii] AID - micromachines-17-01190 [pii] AID - 10.3390/mi17101190 [doi] PST - epublish SO - Micromachines (Basel). 2026 Oct 14;17(10):1190. doi: 10.3390/mi17101190. PMID- 42943945 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Oct 13 TI - Effects of Crosstalk Caused by Finite Extinction Ratio in Optical Time-Division Demultiplexer for PADC. LID - 10.3390/mi17101188 [doi] LID - 1188 AB - Optical time-division demultiplexing photonic analog-to-digital converters (PADCs) represent a compelling paradigm for breaking the bandwidth-resolution trade-off of conventional electronic ADCs, yet inter-channel crosstalk induced by the finite extinction ratio of demultiplexing modulators imposes critical limitations on channel response flatness. Prior investigations have generally ascribed periodic response ripples exclusively to backend bandwidths narrower than half the total sampling rate, while the distortion behavior under wider bandwidths remains inadequately clarified. In this numerical work, we establish a full-link numerical model of a two-channel time-division demultiplexing PADC operating at a total sampling rate of 10 GS/s and systematically characterize crosstalk-induced frequency responses under four representative backend bandwidths (2.5, 5, 7.5, and 10 GHz) with an extinction ratio of 10 dB. Results demonstrate that prominent 10 GHz periodic ripples persist in the channel response even when the backend bandwidth exceeds half the sampling rate. The underlying spectral mechanism is elucidated: demultiplexed comb lines follow a 10 GHz periodic strength distribution, and sampling-generated sidebands inherit the amplitude of their parent comb teeth; the superposition of strong and weak sidebands within the passband governs the ripple magnitude. These findings furnish fundamental insights into crosstalk physics and deliver actionable guidance for device selection and system-level optimization in high-performance PADC implementations. FAU - Chen, Xin AU - Chen X AD - College of Science, National University of Defense Technology, Changsha 410073, China. FAU - Fu, Meicheng AU - Fu M AUID- ORCID: 0000-0002-3101-3088 AD - College of Science, National University of Defense Technology, Changsha 410073, China. FAU - Zhang, Yi AU - Zhang Y AD - College of Science, National University of Defense Technology, Changsha 410073, China. FAU - Zhang, Hongyu AU - Zhang H AD - College of Science, National University of Defense Technology, Changsha 410073, China. FAU - Yu, Hongtao AU - Yu H AD - College of Science, National University of Defense Technology, Changsha 410073, China. FAU - Zhao, Yi AU - Zhao Y AD - College of Science, National University of Defense Technology, Changsha 410073, China. FAU - Yi, Wenjun AU - Yi W AD - College of Science, National University of Defense Technology, Changsha 410073, China. FAU - Li, Xiujian AU - Li X AD - College of Science, National University of Defense Technology, Changsha 410073, China. LA - eng GR - 2025JJ60396/Hunan Provincial Natural Science Foundation of China/ GR - ZK22-17/Research Program of NUDT/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707950 OTO - NOTNLM OT - extinction ratio OT - microwave photonics OT - photonic analog-to-digital converters OT - time-division demultiplexer COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:46 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - mi17101188 [pii] AID - micromachines-17-01188 [pii] AID - 10.3390/mi17101188 [doi] PST - epublish SO - Micromachines (Basel). 2026 Oct 13;17(10):1188. doi: 10.3390/mi17101188. PMID- 42943937 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Oct 12 TI - Construction and Functional Engineering of Bio-Integrated Micro/Nanorobots for Theranostic Applications. LID - 10.3390/mi17101180 [doi] LID - 1180 AB - Bio-integrated micro/nanorobots combine intrinsic biological properties with engineered capabilities, offering promising platforms for biomedical intervention. These systems can exploit biological motility, recognition and therapeutic activity while incorporating controlled navigation and imaging functions. Their development is progressing from active delivery toward theranostic platforms that coordinate transport, localization and treatment. However, integrating actuation, imaging and therapy within a single platform while preserving biological functions remains a major engineering challenge. Here, a framework connecting biological building blocks, construction methods and theranostic requirements is presented. Guided by this framework, engineering strategies based on whole cells and cell-derived components are systematically reviewed to clarify how they establish propulsion, in vivo imaging and therapeutic functions. Emphasis is placed on material selection, integration site, assembly sequence, and opportunities to simplify construction through intrinsic biological functions and multifunctional materials. Despite substantial progress, challenges in biological sourcing, long-term safety and reproducible manufacturing remain barriers to clinical translation. Future directions in personalized construction, standardized production and theranostic integration are outlined. This Review provides guidance on constructing bio-integrated micro/nanorobots for theranostic applications, opening new opportunities for precise intervention and personalized medicine. FAU - Wu, Junfeng AU - Wu J AUID- ORCID: 0009-0001-1069-4267 AD - School of Electrical and Control Engineering, Shenyang Jianzhu University, Shenyang 110168, China. AD - State Key Laboratory of Robotics and Intelligent Systems, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China. FAU - Zhang, Zhaoyu AU - Zhang Z AD - School of Electrical and Control Engineering, Shenyang Jianzhu University, Shenyang 110168, China. FAU - Jiao, Niandong AU - Jiao N AUID- ORCID: 0000-0002-3828-5262 AD - State Key Laboratory of Robotics and Intelligent Systems, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China. FAU - Li, Enkuo AU - Li E AD - School of Electrical and Control Engineering, Shenyang Jianzhu University, Shenyang 110168, China. FAU - Cui, Yuting AU - Cui Y AD - School of Electrical and Control Engineering, Shenyang Jianzhu University, Shenyang 110168, China. FAU - Tong, Mengyao AU - Tong M AD - School of Electrical and Control Engineering, Shenyang Jianzhu University, Shenyang 110168, China. FAU - Zhou, Yan AU - Zhou Y AUID- ORCID: 0000-0001-9527-1361 AD - School of Electrical and Control Engineering, Shenyang Jianzhu University, Shenyang 110168, China. FAU - Liang, Wenfeng AU - Liang W AUID- ORCID: 0000-0002-6605-2511 AD - School of Mechanical Engineering, Shenyang Jianzhu University, Shenyang 110168, China. LA - eng GR - 2023YFB4705600/National Key R&D Program of China/ GR - 62503342/National Natural Science Foundation of China/ GR - U23A20342/National Natural Science Foundation of China/ GR - 62273331/National Natural Science Foundation of China/ GR - 62573305/National Natural Science Foundation of China/ GR - 2024-BSLH-240/Liaoning Provincial Natural Science Foundation/ GR - LJ212510153036/Program of Education Department of Liaoning Province/ GR - 2025-R-089/Liaoning Provincial Archives Project/ GR - HS125120/technical development project under university/ PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707474 OTO - NOTNLM OT - bio-integrated micro/nanorobots OT - biological engineering OT - construction strategies OT - functional integration OT - theranostics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:46 PHST- 2026/09/19 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - mi17101180 [pii] AID - micromachines-17-01180 [pii] AID - 10.3390/mi17101180 [doi] PST - epublish SO - Micromachines (Basel). 2026 Oct 12;17(10):1180. doi: 10.3390/mi17101180. PMID- 42943936 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Oct 11 TI - Polarization-Multiplexed Holography Based on Dielectric Metasurfaces. LID - 10.3390/mi17101179 [doi] LID - 1179 AB - We present a silicon-nanobrick metasurface for dual-channel polarization-multiplexed holography at 1.55 mum. A Jones-matrix design combined with a Fresnel-based Gerchberg-Saxton algorithm maps the target phase profiles to nanobrick dimensions and orientations, enabling combined propagation-phase and geometric-phase control. Numerical simulations demonstrate holographic reconstruction for orthogonal linear, circular, and elliptical polarization pairs. The selected meta-atoms exhibit simulated power transmittances above 70% along both principal axes under periodic boundary conditions. The circular-polarization design is fabricated using electron beam lithography and hard-mask etching, and polarization-selective far-field reconstruction is experimentally observed. Further numerical analysis demonstrates continuous variation of the relative reconstructed intensities along an incident-polarization trajectory. Under an ideal collection and analyzer model, separately optimized elliptical analyzers provide central-signal suppression of 19.02 and 18.78 dB while retaining 64.30% and 61.71% of the corresponding target-image signals. An additional simulated design reconstructs two images within the same spatial region, yielding polarization-channel extinction ratios of 9.30 and 9.33 dB under fixed circular-analyzer conditions. These results demonstrate a silicon-based implementation of dual-channel holographic encoding and provide quantitative characterization of polarization-selective readout. FAU - He, Chen AU - He C AUID- ORCID: 0009-0000-8681-9903 AD - School of Opto-Electronical Engineering, Xi'an Technological University, Xi'an 710021, China. FAU - Wan, Xizhi AU - Wan X AD - School of Opto-Electronical Engineering, Xi'an Technological University, Xi'an 710021, China. FAU - Hou, Jingyao AU - Hou J AD - Xi'an North Electro-Optic Science & Technology Defense Co., Ltd., Xi'an 710043, China. FAU - Tian, Ziyu AU - Tian Z AD - School of Opto-Electronical Engineering, Xi'an Technological University, Xi'an 710021, China. FAU - Wang, Wang AU - Wang W AD - School of Opto-Electronical Engineering, Xi'an Technological University, Xi'an 710021, China. FAU - Kang, Nian AU - Kang N AD - School of Opto-Electronical Engineering, Xi'an Technological University, Xi'an 710021, China. FAU - Zhou, Shun AU - Zhou S AD - School of Opto-Electronical Engineering, Xi'an Technological University, Xi'an 710021, China. FAU - Sun, Xueping AU - Sun X AD - School of Opto-Electronical Engineering, Xi'an Technological University, Xi'an 710021, China. LA - eng GR - No.21JY017/Education Department of Shaanxi Province/ PT - Journal Article DEP - 20261011 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707328 OTO - NOTNLM OT - all-dielectric OT - holographic imaging OT - metasurface OT - phase modulation OT - polarization multiplexing COIS- Jingyao Hou was employed by Xi'an North Electro-Optic Science & Technology Defense Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/11 CRDT- 2026/10/27 10:46 PHST- 2026/09/23 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - mi17101179 [pii] AID - micromachines-17-01179 [pii] AID - 10.3390/mi17101179 [doi] PST - epublish SO - Micromachines (Basel). 2026 Oct 11;17(10):1179. doi: 10.3390/mi17101179. PMID- 42943934 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Oct 11 TI - Precision Trajectory Tracking of Magnetically Actuated Microparticles Using Permanent-Magnet Systems Under State Constraints. LID - 10.3390/mi17101177 [doi] LID - 1177 AB - Magnetically actuated microparticles are promising candidates for minimally invasive microrobotics and targeted delivery in confined microenvironments. Their precise navigation remains challenging because viscous drag, flow disturbances, and safety constraints can prevent accurate trajectory tracking. This paper investigates precision tracking of magnetic microparticles actuated by permanent-magnet systems under state constraints and bounded disturbances. The control problem is formulated at the magnetic-force level, where the force applied to the microparticle is treated as the controlled input. This formulation is motivated by permanent-magnet actuation architectures while remaining applicable when the desired magnetic force can be generated or approximated. An invariant-set-based disturbance rejection strategy is developed. First, an optimized linear controller is obtained using linear matrix inequalities (LMIs) to minimize the output invariant ellipsoid while satisfying state constraints. Then, a homogeneous controller is introduced to improve convergence and tracking accuracy. A worst-case disturbance direction is also characterized. Simulations in a blood-like viscous environment show improved convergence and accuracy with the homogeneous controller, supporting force-level constrained control for precision permanent-magnet actuation of microparticles. FAU - Hamdaoui, Zakaria El AU - Hamdaoui ZE AUID- ORCID: 0009-0006-7326-3890 AD - Institut National des Sciences Appliquees (INSA) Centre Val de Loire, University of Orleans, PRISME EA 4229, 18022 Bourges, France. FAU - Wang, Siyuan AU - Wang S AD - Institut National des Sciences Appliquees (INSA) Centre Val de Loire, University of Orleans, PRISME EA 4229, 18022 Bourges, France. FAU - Boutat, Driss AU - Boutat D AD - Institut National des Sciences Appliquees (INSA) Centre Val de Loire, University of Orleans, PRISME EA 4229, 18022 Bourges, France. FAU - Barbri, Noureddine El AU - Barbri NE AUID- ORCID: 0000-0003-3175-0763 AD - Laboratoire LaSTI, Ecole Nationale des Sciences Appliquees (ENSA) Khouribga, University Sultan Moulay Slimane, Khouribga 25000, Morocco. FAU - Rhofir, Karim AU - Rhofir K AD - Laboratoire LaSTI, Ecole Nationale des Sciences Appliquees (ENSA) Khouribga, University Sultan Moulay Slimane, Khouribga 25000, Morocco. LA - eng GR - CONT 2023 0006/APR IA (Appel a Projets de Recherche d'Initiative Academique)/ PT - Journal Article DEP - 20261011 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707943 OTO - NOTNLM OT - disturbance rejection OT - homogeneous control OT - magnetic microparticles OT - permanent-magnet actuation OT - precision trajectory tracking OT - state constraints COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/11 CRDT- 2026/10/27 10:46 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - mi17101177 [pii] AID - micromachines-17-01177 [pii] AID - 10.3390/mi17101177 [doi] PST - epublish SO - Micromachines (Basel). 2026 Oct 11;17(10):1177. doi: 10.3390/mi17101177. PMID- 42943910 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Sep 30 TI - Area-Selective Atomic Layer Deposition for Next-Generation Semiconductor Processing: Mechanisms of Selectivity Breakdown and Process Integration Strategies. LID - 10.3390/mi17101152 [doi] LID - 1152 AB - As device miniaturization and three-dimensional integration progress, thin-film processes for semiconductor devices increasingly require atomic-level control not only of thickness and composition but also of growth location. Area-selective atomic layer deposition (AS-ALD) is a bottom-up process that exploits differences in surface reactivity between growth and non-growth areas to form thin films only at the intended locations. Because it enables self-aligned patterning and can reduce subsequent etching steps, AS-ALD has drawn attention as a candidate technology for next-generation semiconductor manufacturing. However, the high initial selectivity obtained on planar substrates is not always retained on real patterned or high-aspect-ratio structures. This review examines the process applicability of AS-ALD based on studies published through 2026, focusing on the selectivity window, the maximum selectively deposited thickness, the nucleation density in the non-growth area, and the preservation of pattern shape. Across the literature, the turning point of selectivity breakdown converges on the moment at which the first stable nucleus forms in the non-growth area. Residual surface defects and pinholes in the inhibition layer, together with precursor physisorption, penetration, thermal degradation, and lateral diffusion, all act to bring this transition forward in time. Molecular design strategies (inherent selectivity, self-assembled monolayers, small-molecule inhibitors, surface pretreatment, and combined precursor-inhibitor design) and process control strategies (inhibitor regeneration, ALD/ALE supercycles, and reactor control) are compared according to which stage of the nucleation pathway they intervene in. Pattern- or device-level demonstrations have accumulated for back-end-of-line interconnects and resistive random-access memory, whereas for DRAM, 3D NAND, and gate-all-around transistors most of the evidence remains at the level of material and process concepts. The performance of AS-ALD is difficult to judge from the initial selectivity value alone; a more direct criterion is whether the selectivity window-defined with respect to the target thickness and the tolerable defect level of the actual device structure-can be secured. FAU - Park, Yuna AU - Park Y AD - School of Integrative Engineering, Chung-Ang University, Seoul 06974, Republic of Korea. FAU - Kim, Sooyeon AU - Kim S AD - School of Integrative Engineering, Chung-Ang University, Seoul 06974, Republic of Korea. FAU - Shin, Jinsoo AU - Shin J AD - School of Integrative Engineering, Chung-Ang University, Seoul 06974, Republic of Korea. FAU - Park, Eunmi AU - Park E AD - School of Integrative Engineering, Chung-Ang University, Seoul 06974, Republic of Korea. FAU - Pyo, Sung Gyu AU - Pyo SG AUID- ORCID: 0000-0002-4895-356X AD - School of Integrative Engineering, Chung-Ang University, Seoul 06974, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20260930 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707003 OTO - NOTNLM OT - area-selective atomic layer deposition OT - atomic layer etching OT - inhibitor OT - nucleation OT - selectivity breakdown OT - self-aligned patterning OT - semiconductor process integration COIS- This paper was the result of the research project supported by SK Hynix Inc. The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:46 PHST- 2026/08/06 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - mi17101152 [pii] AID - micromachines-17-01152 [pii] AID - 10.3390/mi17101152 [doi] PST - epublish SO - Micromachines (Basel). 2026 Sep 30;17(10):1152. doi: 10.3390/mi17101152. PMID- 42943906 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Sep 30 TI - A Programmable Delay Line-Based Measurement Methodology for CMOS LiDAR Receivers. LID - 10.3390/mi17101149 [doi] LID - 1149 AB - This paper suggests a novel measurement methodology to precisely evaluate the time-of-flight performance of LiDAR receivers (LRx) by using a CMOS programmable delay line-based time-to-voltage converter (PDL-TVC). Conventional LiDAR systems equip an external laser diode and a photodiode connected via bond wires; however, this causes not only severe signal distortions, but also unpredictable signal delays. In contrast, the proposed PDL-TVC employs a rising edge detector (RED) followed by an integrator and a peak-detect-and-hold (PDH) circuit to convert the time intervals into the output voltage pulses for precise distance estimation, thereby omitting the need of a costly LiDAR transmitter and the corresponding bond wires. In addition, a CMOS optoelectronic LiDAR receiver (COLR) is revealed to prove the proposed test methodology for LRx, comprising an on-chip avalanche photodiode to avoid bond wires, as well as an active feedback transimpedance amplifier followed by a Schmitt trigger to generate STOP signals to determine the time intervals from START pulses. Furthermore, a 4-to-10 decoder is added to provide externally selectable delay intervals of 1-10 ns, enabling the controlled test of the PDL-TVC operations. The proposed COLR chip is fabricated in a 180 nm CMOS process, occupying 312 x 659 mum(2) for the core area. The PDL-TVC consumes 12 mW, with the programmable delay line consuming 374 microW under a 1.8 V supply. Measured results of the COLR confirms reliable on-chip signal generation and accurate characterization, thus demonstrating that the proposed measurement methodology using the PDL-TVC offers a precise solution for evaluating LiDAR receivers. FAU - Seo, Bobin AU - Seo B AUID- ORCID: 0009-0006-6459-4735 AD - Division of Electronic and Semiconductor Engineering, Ewha Womans University, Seoul 03760, Republic of Korea. FAU - Lee, Sunkyung AU - Lee S AD - Division of Electronic and Semiconductor Engineering, Ewha Womans University, Seoul 03760, Republic of Korea. FAU - Park, Somi AU - Park S AD - Division of Electronic and Semiconductor Engineering, Ewha Womans University, Seoul 03760, Republic of Korea. FAU - Park, Sung-Min AU - Park SM AUID- ORCID: 0000-0003-2653-9955 AD - Division of Electronic and Semiconductor Engineering, Ewha Womans University, Seoul 03760, Republic of Korea. LA - eng GR - IITP-2026-RS-2020-II201847/Ministry of Science and ICT/ GR - RS-2025-25442252/National Research Foundation of Korea/ PT - Journal Article DEP - 20260930 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707903 OTO - NOTNLM OT - CMOS OT - LiDAR OT - TIA OT - TVC OT - programmable delay line COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:46 PHST- 2026/07/28 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - mi17101149 [pii] AID - micromachines-17-01149 [pii] AID - 10.3390/mi17101149 [doi] PST - epublish SO - Micromachines (Basel). 2026 Sep 30;17(10):1149. doi: 10.3390/mi17101149. PMID- 42943904 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Sep 30 TI - Quasi-Dry Electrochemical Mechanical Polishing Method of Additively Manufactured Parts. LID - 10.3390/mi17101147 [doi] LID - 1147 AB - Selective laser melting (SLM) of TC4 titanium alloy inevitably introduces surface defects including spheroidized particles, adhered powders, and microcracks, resulting in high roughness and poor flatness that prevent direct service. Electrochemical polishing (ECP) is suitable for complex curved surfaces, yet conventional immersion ECP consumes substantial acidic electrolytes, leading to waste and pollution. To overcome this, we propose a quasi-dry electrochemical mechanical polishing (Q-DECMP) method. Millimeter-scale abrasives serve as carriers, and an ethylene glycol-based NaCl electrolyte is coated onto the abrasive surfaces at an electrolyte-to-abrasive mass ratio of 1:20, existing only as a trace liquid film instead of immersing the workpiece. Owing to their high viscosity and capillary forces, a stable conductive liquid film forms on the abrasives. Since the abrasive size (2 mm) is larger than the microscopic peak-valley height of the SLM surface, the film-covered abrasives preferentially dissolve the peaks while the valleys remain unattacked, achieving selective leveling. Experiments performed at 200 V, 1 m/s linear velocity, and 6.5 mm interelectrode gap for 40 min show that Ra decreases from 6.89 to 0.77 mum (88.8% reduction) and material removal rate reaches 612.5 mug/min, outperforming pure mechanical polishing (Ra 4.98 mum, MRR 220 mug/min). With minimal electrolyte usage, this method reduces electrolyte consumption compared with conventional immersion electrochemical polishing, enabling efficient and low-loss finishing. FAU - Han, Shihao AU - Han S AD - College of Mechanical and Electrical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China. FAU - Sun, Zhiguang AU - Sun Z AD - College of Mechanical and Electrical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China. FAU - Hu, Xiaoyun AU - Hu X AD - College of Mechanical and Electrical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China. LA - eng GR - 52675576 and U24A20127/National Natural Science Foundation of China/ GR - NP2025117/Fundamental Research Funds for the Central Universities/ GR - KYCX25_0600/Postgraduate Research & Practice Innovation Program of Jiangsu Province/ GR - SYJS202601Z and SYJS202504Z/Experimental Technology Research and Development of Nanjing University of Aeronautics and Astronautics/ PT - Journal Article DEP - 20260930 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707867 OTO - NOTNLM OT - TC4 titanium alloy OT - electrochemical mechanical polishing OT - selective laser melting OT - surface roughness COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:46 PHST- 2026/09/01 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - mi17101147 [pii] AID - micromachines-17-01147 [pii] AID - 10.3390/mi17101147 [doi] PST - epublish SO - Micromachines (Basel). 2026 Sep 30;17(10):1147. doi: 10.3390/mi17101147. PMID- 42943900 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Sep 30 TI - Wire Electrochemical Micro Machining of Narrow Slits on Fe-Based Amorphous Alloys. LID - 10.3390/mi17101143 [doi] LID - 1143 AB - Amorphous alloys (metallic glasses) possess unique long-disordered but short-ordered atomic structures and outstanding mechanical properties, including high hardness, high strength, and good corrosion resistance, making them attractive for precision microdevice applications. However, their poor machinability limits their practical deployment because conventional thermomechanical processing can readily cause crystallization-related defects due to heat input. Wire electrochemical micromachining (WECM), based on anodic dissolution, generates no cutting heat or tool electrode wear and thus prevents crystallization damage during the fabrication of complex microstructures. This study systematically explores the electrochemical dissolution behavior and narrow-slit machining performance of Fe-based amorphous alloys. Slit-cutting experiments are conducted to reveal the effects of key parameters, including electrolyte type, feed rate, and electrical parameters, on machining characteristics. Following single-factor experimental investigation and parameter selection, 0.2 mol/L sulfuric acid is selected as the optimal electrolyte. The optimal parameters are a machining voltage of 7 V, a pulse width of 200 ns, a feed rate of 0.2 mum/s, and a wire electrode vibration amplitude of 100 mum. Uniform narrow slits with good surface quality are achieved. Representative complex microstructures are successfully fabricated. This work verifies the feasibility of WECM for the microfabrication of Fe-based amorphous alloys and advances its potential applications in microelectromechanical systems (MEMS) and aerospace components. FAU - Hang, Yusen AU - Hang Y AD - College of Mechanical Engineering, Nanjing University of Industry Technology, Nanjing 210023, China. AD - Jiangsu Provincial Key Laboratory of Precision and Micro-Manufacturing Technology, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China. AD - Precision Manufacturing Engineering and Technology Research Center of Jiangsu Province, Nanjing University of Industry Technology, Nanjing 210023, China. FAU - Yang, Tao AU - Yang T AUID- ORCID: 0000-0002-4275-6143 AD - College of Mechanical Engineering, Nanjing University of Industry Technology, Nanjing 210023, China. AD - Precision Manufacturing Engineering and Technology Research Center of Jiangsu Province, Nanjing University of Industry Technology, Nanjing 210023, China. FAU - Wu, Xiujuan AU - Wu X AD - College of Mechanical Engineering, Nanjing University of Industry Technology, Nanjing 210023, China. FAU - Kong, Weijing AU - Kong W AD - College of Mechanical Engineering, Nanjing University of Industry Technology, Nanjing 210023, China. LA - eng GR - JSKL2324K02/Jiangsu Key Laboratory of Precision and Micro-Manufacturing Technology/ GR - YK240102/Start-up Fund for New Talented Researchers of Nanjing University of Industry Technology/ GR - 05SJGCZX-01/Jiangsu Province Precision Manufacturing Engineering and Technology Research Center/ GR - No/Qing Lan Project/ PT - Journal Article DEP - 20260930 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707141 OTO - NOTNLM OT - Fe-based amorphous alloy OT - electrochemical characteristics OT - microstructure OT - narrow-slit process optimization OT - wire electrochemical micromachining COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:46 PHST- 2026/08/27 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - mi17101143 [pii] AID - micromachines-17-01143 [pii] AID - 10.3390/mi17101143 [doi] PST - epublish SO - Micromachines (Basel). 2026 Sep 30;17(10):1143. doi: 10.3390/mi17101143. PMID- 42943896 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Sep 30 TI - RFEA-Derived Ion-Energy Descriptors for Preliminary Process-Window Evaluation in Dual-Beam Soft Plasma Etching of Organic Semiconductor Films. LID - 10.3390/mi17101139 [doi] LID - 1139 AB - Low-damage plasma processing of organic semiconductor films requires a consistent way to relate plasma diagnostics to material responses. This study presents a retarding-field energy analyser (RFEA)-guided workflow for preliminary evaluation of seven nominal single- and dual-beam settings. A selectivity-ratio (SR) screening index was calculated as the ratio of integrated ion-energy-distribution intensity in fixed 4-6 eV and 6-8 eV windows. The seven recorded scores range from 1.32 to 7.58, with the 50 + 100 condition label giving the highest window ratio. The index describes the relative spectral weight of the two intervals under an assumed activation-versus-damage interpretation. A published effective-energy model provides separate physical context. Time-resolved optical measurements and baseline electrical characterisation were complemented by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). At 100 Pa for 100 s, dual-beam treatment showed higher mean surface O/C while retaining mean roughness close to the unetched films. Additional stylus profilometry comprised 42 scans from 14 specimens. At 50 Pa for 180 s, treated-group mean apparent step heights ranged from 160.7 to 205.9 nm, without a uniform increase under source superposition. These within-protocol measurements provide complementary criteria for assessing candidate conditions. The workflow combines an explicit spectral ranking with measured film responses for preliminary process-window evaluation; SR is interpreted as a screening index rather than a measured molecular etch-rate ratio. FAU - Gao, Rui AU - Gao R AD - Department of Medical Engineering, Second Affiliated Hospital, Army Medical University, Chongqing 400030, China. FAU - Dai, Mengna AU - Dai M AD - Department of Medical Engineering, Second Affiliated Hospital, Army Medical University, Chongqing 400030, China. FAU - Zou, Xin AU - Zou X AD - Department of Medical Engineering, Second Affiliated Hospital, Army Medical University, Chongqing 400030, China. FAU - Wang, Yuheng AU - Wang Y AUID- ORCID: 0000-0001-6394-1455 AD - Department of Medical Engineering, Second Affiliated Hospital, Army Medical University, Chongqing 400030, China. LA - eng GR - 52507281/National Natural Science Foundation of China/ GR - QNBS048/Xinqiao Hospital/ PT - Journal Article DEP - 20260930 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707040 OTO - NOTNLM OT - RFEA OT - X-ray photoelectron spectroscopy OT - atomic force microscopy OT - candidate-condition ranking OT - dual-beam modulation OT - ion-energy-distribution function OT - organic semiconductor OT - soft plasma etching COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:46 PHST- 2026/05/31 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - mi17101139 [pii] AID - micromachines-17-01139 [pii] AID - 10.3390/mi17101139 [doi] PST - epublish SO - Micromachines (Basel). 2026 Sep 30;17(10):1139. doi: 10.3390/mi17101139. PMID- 42943894 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Sep 29 TI - Three Minimum Variance Distortionless Response Algorithms Based on Quaternion Model for Single MEMS Vector Hydrophone. LID - 10.3390/mi17101137 [doi] LID - 1137 AB - To address the limitations of the traditional long-vector model, which cannot adequately preserve the inherent orthogonal structure among channels in the signal processing of a single vector hydrophone, three minimum variance distortionless response (MVDR) direction-of-arrival estimation algorithms based on the quaternion model are proposed for a single MEMS vector hydrophone. Compared with the traditional long-vector model, the quaternion can retain the structural relationship between channels in a more compact data representation form. First, based on the output characteristics of the sound pressure and dual-channel vibration velocity of the two-dimensional MEMS vector hydrophone, three quaternion signal models-namely Q-VV, Q-PVV, and Q-PV-are constructed. Subsequently, the corresponding MVDR spatial spectra are derived in the quaternion domain. To enhance the direction-of-arrival resolution performance under low-signal-to-noise-ratio (SNR) conditions, the algorithms are then improved by incorporating subspace decomposition theory. The simulation results show that the three proposed quaternion-based MVDR algorithms can all realize effective direction-of-arrival estimation for a single target. When the signal-to-noise ratio is greater than -4 dB, the direction estimation error is less than 1 degrees . The results of dynamic bearing trajectory tests and fixed-point direction-of-arrival estimation experiments indicate that the proposed method can realize the continuous and stable tracking of the target azimuth. Among the involved algorithms, Q-PV-MVDR can effectively suppress false targets, with an average relative angle orientation error of 0.5 degrees . FAU - Zhu, Shan AU - Zhu S AUID- ORCID: 0000-0001-6680-7200 AD - Department of Automation, Taiyuan Institute of Technology, Taiyuan 030008, China. FAU - Geng, Yanan AU - Geng Y AD - State key Laboratory of Extreme Environment Optoelectronic Dynamic Measurement Technology and Instrument, North University of China, Taiyuan 030051, China. FAU - Mu, Keqiang AU - Mu K AD - North Automatic Control Technology Institute, Taiyuan 030006, China. FAU - Li, Yongwei AU - Li Y AD - Department of Automation, Taiyuan Institute of Technology, Taiyuan 030008, China. FAU - Li, Feng AU - Li F AD - Department of Automation, Taiyuan Institute of Technology, Taiyuan 030008, China. FAU - Gao, Zhiqiang AU - Gao Z AUID- ORCID: 0009-0008-9748-3577 AD - Department of Automation, Taiyuan Institute of Technology, Taiyuan 030008, China. FAU - Zhang, Yunyue AU - Zhang Y AD - Department of Automation, Taiyuan Institute of Technology, Taiyuan 030008, China. FAU - Zhao, Zhicheng AU - Zhao Z AD - Department of Automation, Taiyuan Institute of Technology, Taiyuan 030008, China. LA - eng GR - 2024L362/Scientific and Technologial Innovation Programs of Higher Education Institutions in Shanxi/ GR - 202403021222291/Basic Research Program of Shanxi Province/ GR - 202503021212300/Basic Research Program of Shanxi Province/ GR - 202401060301011/Shanxi Province Major Science and Technology Special Project "Open Competition Mechanism to Select the Best Candidates" Project/ PT - Journal Article DEP - 20260929 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707090 OTO - NOTNLM OT - MVDR OT - direction-of-arrival estimation OT - quaternion OT - single MEMS vector hydrophone COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/29 CRDT- 2026/10/27 10:46 PHST- 2026/07/07 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/29 00:00 [pmc-release] AID - mi17101137 [pii] AID - micromachines-17-01137 [pii] AID - 10.3390/mi17101137 [doi] PST - epublish SO - Micromachines (Basel). 2026 Sep 29;17(10):1137. doi: 10.3390/mi17101137. PMID- 42943893 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Sep 29 TI - Universal Parafilm((R))-Mediated Thermal Bonding for Gold Leaf Electrode Patterning and Microfluidic Assembly on Diverse Substrates. LID - 10.3390/mi17101136 [doi] LID - 1136 AB - Gold leaf electrodes offer a low-cost, substrate-versatile strategy for electrochemical sensing and microfluidic systems. Herein, we demonstrate a unified Parafilm((R))-mediated platform in which the Parafilm((R)) layer serves as both the adhesive for gold-leaf electrode patterning and the bonding/channel-defining layer for microfluidic assembly via heat-activated bonding. Gold leaf electrodes were patterned on diverse substrates by laser ablation, achieving a minimum achieved linewidth of 53 +/- 3 mum on polyethylene terephthalate (PET) sheets. Electrochemical characterization showed stable CV responses after 40 manual bending cycles and good batch reproducibility. Leveraging Parafilm's hydrophobicity and thermoplasticity, we integrated electrodes into hierarchical microfluidic architectures on paper and PET. Paper-based hybrid devices with asymmetric self-driven channels enabled dual-zone measurements. Structured PET microchannels yielded microfluidic devices with sequential flow control and selective loading. In a proof-of-concept H(2)O(2) assay, on-chip Prussian blue electrodeposition followed by amperometry gave a detection limit of 0.44 mg/L and ~85% recovery in spiked pond water. This Parafilm((R))-mediated strategy provides a low-cost route for cross-substrate microfluidic-electrochemical integration, unifying electrode fabrication and device assembly on a single thermoplastic bonding platform. FAU - Yang, Zhiyuan AU - Yang Z AD - NHC Key Laboratory of Tropical Disease Control, School of Life Sciences and Medical Technology, Hainan Medical University, Haikou 571199, China. FAU - Lou, Yafei AU - Lou Y AD - State Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510640, China. FAU - Chen, Siyu AU - Chen S AD - State Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510640, China. FAU - Chen, Yongye AU - Chen Y AD - State Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510640, China. FAU - Sun, Linan AU - Sun L AD - State Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510640, China. FAU - Tian, Junfei AU - Tian J AD - State Key Laboratory of Pulp and Paper Engineering, School of Light Industry and Engineering, South China University of Technology, Guangzhou 510640, China. FAU - Cao, Rong AU - Cao R AD - NHC Key Laboratory of Tropical Disease Control, School of Life Sciences and Medical Technology, Hainan Medical University, Haikou 571199, China. LA - eng GR - 82160404/National Natural Science Foundation of China/ GR - 82572364/National Natural Science Foundation of China/ GR - ZDYF2021SHFZ235/Department of Science and Technology of Hainan Province/ PT - Journal Article DEP - 20260929 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707072 OTO - NOTNLM OT - Parafilm(R) sheet OT - electrochemical detection OT - gold leaf electrode OT - microfluidic device OT - paper-based sensor COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/29 CRDT- 2026/10/27 10:46 PHST- 2026/08/17 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/29 00:00 [pmc-release] AID - mi17101136 [pii] AID - micromachines-17-01136 [pii] AID - 10.3390/mi17101136 [doi] PST - epublish SO - Micromachines (Basel). 2026 Sep 29;17(10):1136. doi: 10.3390/mi17101136. PMID- 42943885 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Sep 28 TI - Noise-Aware Bayesian Optimization for Precision Improvement of Micro-Volume Liquid Handling in In Vitro Diagnostics. LID - 10.3390/mi17101128 [doi] LID - 1128 AB - Micro-volume dispensing precision is critical for in vitro diagnostic (IVD) analyzers. We present a noise-aware Bayesian optimization framework that minimizes the within-run coefficient of variation (CV) of a 50 muL dispensing process by optimizing five pump-control variables. Each setting was tested in five independent batches; the group mean CV was used as the response, and the squared group standard error was supplied to an automatic relevance determination Gaussian process as observation-noise variance. From 33 tested combinations, the lowest measured mean CV was 0.266% (SD, 0.044%). An engineering-rounded setting then achieved 0.270% (SD, 0.050%), an 81.8% relative reduction versus engineer-selected settings (1.480%, SD, 0.083%). In a retrospective surrogate-based replay, the noise-aware strategy reached CV < 1.5% in 2.8 +/- 1.2 iterations, compared with 4.1 +/- 2.0, 6.5 +/- 3.4, and 11.3 +/- 5.1 for homoscedastic GP, standard GP, and random search. Deionized water, diluted human serum, and 5% bovine serum albumin all yielded mean CVs below 0.45%. The method thus identified a repeatable low-CV operating region with a limited physical-experiment budget; prospective algorithmic comparisons and multi-instrument validation remain necessary. FAU - Bai, Lihao AU - Bai L AD - Kaihe Biotechnology (Shanghai) Co., Ltd., Shanghai 200120, China. LA - eng PT - Journal Article DEP - 20260928 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707286 OTO - NOTNLM OT - Bayesian optimization OT - Gaussian process OT - coefficient of variation OT - heteroscedastic noise OT - in vitro diagnostics OT - microliter dispensing COIS- Author Lihao Bai was employed by the company Kaihe Biotechnology (Shanghai) Co., Ltd. The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/28 CRDT- 2026/10/27 10:46 PHST- 2026/09/03 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - mi17101128 [pii] AID - micromachines-17-01128 [pii] AID - 10.3390/mi17101128 [doi] PST - epublish SO - Micromachines (Basel). 2026 Sep 28;17(10):1128. doi: 10.3390/mi17101128. PMID- 42943879 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Sep 25 TI - Electrochemical and Optical Biosensors for Acetylcholine Monitoring in Neurodegeneration. LID - 10.3390/mi17101122 [doi] LID - 1122 AB - Acetylcholine (ACh) plays a pivotal role in cognition, learning, memory, and neuronal plasticity, and alterations in cholinergic neurotransmission are closely associated with neurodegenerative disorders, particularly Alzheimer's disease and related dementias. Consequently, the development of analytical tools capable of accurately monitoring ACh has attracted considerable interest in both neuroscience and clinical research. However, the determination of ACh remains challenging because of its low physiological concentrations, rapid enzymatic degradation, and the complexity of biological matrices. This review provides a comprehensive overview of recent advances in electrochemical and optical biosensors developed for ACh detection, with particular emphasis on progress reported between 2020 and 2026. The sensing principles, analytical performance, and technological characteristics of enzymatic and non-enzymatic electrochemical platforms, as well as colorimetric, fluorescent, surface plasmon resonance, and emerging molecular optical sensors, are critically discussed. Particular attention is given to the role of nanomaterials, signal-amplification strategies, miniaturized devices, wearable and implantable platforms, and approaches enabling real-time monitoring. Recent studies demonstrate remarkable improvements in sensitivity and detection limits; however, translation towards clinical applications remains limited by challenges related to selectivity in complex biological samples, biofouling, long-term stability, reproducibility, and the scarcity of validation studies involving authentic patient-derived specimens. Overall, current advances indicate a shift from proof-of-concept sensor development towards application-oriented platforms aimed at continuous neurochemical monitoring and point-of-care analysis. Future progress will depend on achieving robust performance in physiologically relevant environments and establishing clinically validated biosensing strategies for the assessment of cholinergic dysfunction in neurodegenerative diseases. FAU - Hervas-Perez, Juan Pablo AU - Hervas-Perez JP AUID- ORCID: 0000-0003-3234-0993 AD - Department of Chemistry in Pharmaceutical Sciences, Faculty of Pharmacy, Complutense University of Madrid, 28040 Madrid, Spain. FAU - Munoz-Oporto, Laura AU - Munoz-Oporto L AD - Department of Chemistry in Pharmaceutical Sciences, Faculty of Pharmacy, Complutense University of Madrid, 28040 Madrid, Spain. FAU - Moreno-Guzman, Maria AU - Moreno-Guzman M AUID- ORCID: 0000-0003-3761-0542 AD - Department of Chemistry in Pharmaceutical Sciences, Faculty of Pharmacy, Complutense University of Madrid, 28040 Madrid, Spain. FAU - Sanchez-Paniagua, Marta AU - Sanchez-Paniagua M AUID- ORCID: 0000-0001-5617-4788 AD - Department of Chemistry in Pharmaceutical Sciences, Faculty of Pharmacy, Complutense University of Madrid, 28040 Madrid, Spain. LA - eng PT - Journal Article PT - Review DEP - 20260925 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707057 OTO - NOTNLM OT - Alzheimer's disease OT - acetylcholine OT - cholinergic dysfunction OT - electrochemical biosensing OT - nanomaterials OT - neurodegenerative diseases OT - neurotransmitter monitoring OT - optical biosensing OT - point-of-care diagnostics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/25 CRDT- 2026/10/27 10:46 PHST- 2026/09/03 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - mi17101122 [pii] AID - micromachines-17-01122 [pii] AID - 10.3390/mi17101122 [doi] PST - epublish SO - Micromachines (Basel). 2026 Sep 25;17(10):1122. doi: 10.3390/mi17101122. PMID- 42943877 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Sep 25 TI - Magnetically Actuated Hydrogel-Based Microrobotic Structures with X-Ray Visibility for Targeted Drug Delivery in Vascular Environments. LID - 10.3390/mi17101120 [doi] LID - 1120 AB - In recent years, microrobots have attracted attention as minimally invasive therapeutic tools in biomedical applications, as they enable enhanced drug delivery efficiency through direct and localized transport. However, practical realization requires highly precise controllability and real-time responsiveness. In this study, we present a hydrogel-based microrobotic structure precisely and dynamically controlled in real time using externally applied magnetic fields. Chemical, structural, and magnetic characteristics were systematically analyzed, followed by experimental evaluation of targeting performance within a vascular-mimicking model under a phosphate-buffered saline (PBS) environment. Results demonstrate that sufficient visibility in X-ray imaging can be achieved depending on the concentration of embedded magnetic nanoparticles introduced during fabrication. Moreover, stable and real-time targeted navigation of the microrobotic structures was confirmed within specific threshold ranges of magnetic field strength and frequency. By effectively restricting off-target motion, the proposed system offers a minimally invasive medical platform that simultaneously ensures high stability and control precision. FAU - Jung, Jiyu AU - Jung J AD - Department of Mechanical Engineering, Chosun University, 309 Pilmun-daero, Dong-gu, Gwangju 61452, Republic of Korea. FAU - Mun, Geonhui AU - Mun G AD - Department of Mechanical Engineering, Chosun University, 309 Pilmun-daero, Dong-gu, Gwangju 61452, Republic of Korea. FAU - Sung, Yoon-Gyung AU - Sung YG AUID- ORCID: 0000-0001-8096-2389 AD - Department of Mechanical Engineering, Chosun University, 309 Pilmun-daero, Dong-gu, Gwangju 61452, Republic of Korea. FAU - Lee, Kyongsu AU - Lee K AD - Department of Mechanical Engineering, Chosun University, 309 Pilmun-daero, Dong-gu, Gwangju 61452, Republic of Korea. FAU - Nguyen, Van Du AU - Nguyen VD AUID- ORCID: 0000-0001-5139-8491 AD - Department of Mechanical Engineering, Sogang University, 35, Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea. FAU - Go, Gwangjun AU - Go G AD - Department of Mechanical Engineering, Chosun University, 309 Pilmun-daero, Dong-gu, Gwangju 61452, Republic of Korea. FAU - Choi, Eunpyo AU - Choi E AUID- ORCID: 0000-0002-9689-6520 AD - Department of Mechanical Engineering, Sogang University, 35, Baekbeom-ro, Mapo-gu, Seoul 04107, Republic of Korea. LA - eng GR - 2026-ANCHOR-05-013/Ministry of Education/ PT - Journal Article DEP - 20260925 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13706811 OTO - NOTNLM OT - X-ray visibility OT - electromagnetic actuation OT - intravascular navigation OT - magnetic microrobots OT - magnetic nanoparticle concentration OT - targeted drug delivery COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/25 CRDT- 2026/10/27 10:46 PHST- 2026/08/24 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - mi17101120 [pii] AID - micromachines-17-01120 [pii] AID - 10.3390/mi17101120 [doi] PST - epublish SO - Micromachines (Basel). 2026 Sep 25;17(10):1120. doi: 10.3390/mi17101120. PMID- 42943876 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Sep 24 TI - Design of a 3D Aggregation Backbone Network-on-Chip Architecture for High-Speed Data Acquisition Systems. LID - 10.3390/mi17101119 [doi] LID - 1119 AB - Time-interleaved high-speed data acquisition (HS-DAQ) systems based on three-dimensional network-on-chip (3D NoC) architectures require efficient transmission of multi-channel sampled data. However, existing general-purpose NoC architectures are not well suited for the deterministic multi-source aggregation communication pattern of HS-DAQ systems, which may result in increased long-distance transmission overhead and localized traffic congestion. To address these issues, this paper proposes a 3D aggregation backbone network-on-chip architecture (3D AB-NoC) for HS-DAQ systems. This architecture is based on a 3D Mesh structure and enables the rapid transmission of long-distance aggregated data by establishing coarse-grained aggregation backbone subnets between defined aggregation routing nodes. Furthermore, a Backbone-Boosted XYZ (BB-XYZ) routing algorithm is proposed, which selects the next hop based on the remaining transmission distance of both the basic mesh path and the backbone path, thereby achieving coordinated routing for both local transmission and long-distance aggregation. Simulation results based on the Access Noxim platform demonstrate that the 3D AB-NoC maintains stable basic communication performance under general communication scenarios. In HS-DAQ communication scenarios, the proposed architecture reduces average latency by up to approximately 44%, improves throughput by approximately 29.3%, and significantly suppresses the growth of maximum latency under high-load conditions. FAU - Deng, Yunhui AU - Deng Y AD - School of Electronic Engineering and Automation, Guilin University of Electronic Technology, Guilin 541004, China. FAU - Xu, Chuanpei AU - Xu C AD - School of Electronic Engineering and Automation, Guilin University of Electronic Technology, Guilin 541004, China. AD - Guangxi Key Laboratory of Automatic Detecting Technology and Instruments, Guilin 541004, China. FAU - Mo, Wei AU - Mo W AD - School of Electronic Engineering and Automation, Guilin University of Electronic Technology, Guilin 541004, China. AD - Guangxi Key Laboratory of Automatic Detecting Technology and Instruments, Guilin 541004, China. FAU - Wan, Chunting AU - Wan C AUID- ORCID: 0000-0001-5568-767X AD - School of Electronic Engineering and Automation, Guilin University of Electronic Technology, Guilin 541004, China. AD - Guangxi Key Laboratory of Automatic Detecting Technology and Instruments, Guilin 541004, China. LA - eng GR - 62161008/National Natural Science Foundation of China/ GR - GuiKeLT2600640021/Guangxi Key Laboratory of Automatic Detecting Technology and Instruments/ PT - Journal Article DEP - 20260924 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707338 OTO - NOTNLM OT - aggregation backbone architecture OT - deterministic routing OT - high-speed data acquisition system OT - network-on-chip COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:46 PHST- 2026/08/19 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - mi17101119 [pii] AID - micromachines-17-01119 [pii] AID - 10.3390/mi17101119 [doi] PST - epublish SO - Micromachines (Basel). 2026 Sep 24;17(10):1119. doi: 10.3390/mi17101119. PMID- 42943875 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Sep 24 TI - Protein-Based Biomaterials for Bio-Optoelectronics: A Review of Bio-Integrated and Biodegradable Optodevices. LID - 10.3390/mi17101118 [doi] LID - 1118 AB - Growing development of biomaterials has stimulated the biomedical sector to seek innovative alternatives to conventional medical technologies. Bio-optoelectronic devices are emerging as promising tools, integrating biologically derived components, biodegradable end-of-life approaches, and highly biocompatible materials with optoelectronic systems. The devices combine optical and electronic properties with biological interfaces, enabling more effective interaction with tissues and cellular systems. Applied research is motivated by the need to improve the efficacy and biocompatibility of medical devices while reducing invasiveness and adverse effects. Wearable devices and implants based on flexible, biodegradable, or bioinspired materials allow better adaptation to biological tissues and real-time monitoring or modulation of physiological processes. Proteins are attracting increasing attention due to their biocompatibility, biodegradability, mechanical flexibility, and ability to respond to optical and electrical stimuli. Furthermore, the combination of proteins with synthetic materials and nanomaterials in biohybrid platforms offers new opportunities for advanced bio-optoelectronic systems. This review summarizes key developments in bio-optoelectronic devices, focusing on protein-based materials; their roles in sensing, imaging, and neuromodulation; and their use in wearable, implantable, and biotech systems. Crucial challenges, including long-term stability and integration with biological systems, are also discussed, together with future perspectives for diagnostic and therapeutic applications. FAU - Merlo, Anna AU - Merlo A AD - Department of Pharmaceutical Sciences, University of Padova, Via Marzolo, 5, 35131 Padova, Italy. FAU - Conti, Fosca AU - Conti F AUID- ORCID: 0000-0003-4424-1474 AD - Department of Chemical Sciences, University of Padova, Via Marzolo 1, 35131 Padova, Italy. LA - eng PT - Journal Article PT - Review DEP - 20260924 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707806 OTO - NOTNLM OT - biodevice OT - biohybrid OT - bioimplants OT - biomedical OT - biosensor OT - biotech OT - imaging OT - neuromodulation OT - protein OT - sensing COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:46 PHST- 2026/07/30 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - mi17101118 [pii] AID - micromachines-17-01118 [pii] AID - 10.3390/mi17101118 [doi] PST - epublish SO - Micromachines (Basel). 2026 Sep 24;17(10):1118. doi: 10.3390/mi17101118. PMID- 42943871 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Sep 24 TI - Effects of Structural and Electrical Defects on Vibration Suppression and Energy Harvesting Performance of Piezoelectric Metamaterial Beam. LID - 10.3390/mi17101114 [doi] LID - 1114 AB - This paper investigates the vibration suppression and energy harvesting performance of a locally resonant piezoelectric metamaterial beam (PMMB) with structural and electrical defects. The unit cell of a PMMB comprises a square aluminum substrate, two rubber plates sandwiching a piezoelectric plate, and a cylindrical stub on top. The structural and electrical defects are introduced by varying the material density of a stub, adjusting the spacing between the two adjacent piezoelectric local resonators (PLRs), and connecting inductors to selected PLRs, respectively. To investigate the theoretical band gap (BG), the effective BG (i.e., attenuation band), and the energy harvesting performance of defect-configured PMMB, supercell finite element were performed to obtain dispersion curves, while the six-PLR PMMB simulations were conducted to acquire transmission spectra and responding voltage outputs. Three distinct mechanisms are explored to enhance the performance of PMMB: defect states inside the effective BG for energy localization, defect-boundary interference for energy redistribution in the passband, and LC resonance in the shunted piezoelectric resonators for frequency-tunable energy harvesting. The results demonstrate that either stub-mass-reduced defect or substrate-length-increased spacing defect yield high voltages via energy localization in the effective BG, but they also narrow the effective BG. In contrast, tuning the center-to-center spacing of the two adjacent PLRs (near the excitation end) to an appropriate distance generates a dominant passband peak. The significant rises in both the far-end PLR voltage and the total voltage of the six PLRs clearly indicate energy redistribution within the passband. By selecting appropriate inductor parameters, LC-resonance-based electrical defects enable frequency-tunable voltage enhancement either at the effective BG edge or within the passband. Overall, this study provides comprehensive insight into how structural and electrical defects affect the response characteristics of the locally resonant PMMB. FAU - Wang, Hongyan AU - Wang H AD - College of Mechatronic Engineering, Qiqihar University, Qiqihar 161006, China. FAU - Jin, Xinru AU - Jin X AD - College of Mechatronic Engineering, Qiqihar University, Qiqihar 161006, China. FAU - Wang, Guanglei AU - Wang G AD - College of Mechatronic Engineering, Qiqihar University, Qiqihar 161006, China. FAU - Sun, Xiangwei AU - Sun X AD - College of Mechatronic Engineering, Qiqihar University, Qiqihar 161006, China. LA - eng GR - PL2024E036/Heilongjiang Provincial Natural Science Foundationn of China/ GR - QUZLTS_CX2025007; QUZLTS_CX2026017/Graduate Innovation Research Projects in Qiqihar University of China/ PT - Journal Article DEP - 20260924 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13706957 OTO - NOTNLM OT - electrical defects OT - energy harvesting OT - metamaterial beam OT - piezoelectric local resonators OT - structural defects OT - vibration suppression COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:46 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - mi17101114 [pii] AID - micromachines-17-01114 [pii] AID - 10.3390/mi17101114 [doi] PST - epublish SO - Micromachines (Basel). 2026 Sep 24;17(10):1114. doi: 10.3390/mi17101114. PMID- 42943870 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Sep 24 TI - A Broadband Microwave Absorber Operating Under High-Power Antenna Irradiation. LID - 10.3390/mi17101113 [doi] LID - 1113 AB - A microwave absorber is important for reducing the scattering of airborne antennas and radio-frequency systems. However, absorbed electromagnetic energy is inevitably converted into heat, and high-power antenna irradiation may cause local temperature rise and safety hazards. In this work, we demonstrated how to overcome this bottleneck to be compatible with broadband absorption and to work under high-power electromagnetic irradiation. Through the analysis of the electromagnetic-thermal characteristics of typical periodic structural units, cross-shaped and square-ring resistive-film patterns were chosen to design a broadband absorber. Finally, a dual-layer structure consisting of a lower square-ring resistive-film layer and an upper cross-shaped resistive-film layer was designed using equivalent-circuit modeling and full-wave simulation. The fabricated absorber exhibited a measured reflection response below -10 dB from 5.1 to 18 GHz. When the absorber is illuminated by a high-power antenna, its steady-state temperature is only 59.7 degrees C. These results indicate that the proposed scheme can balance broadband microwave absorption and thermal-management capability, providing a feasible route for absorbers used in high-power antenna irradiation environments. FAU - Yu, Wenjun AU - Yu W AD - Chengdu Aircraft Industrial (Group) Co., Ltd., Chengdu 610073, China. FAU - Yang, Dong AU - Yang D AD - Chengdu Aircraft Industrial (Group) Co., Ltd., Chengdu 610073, China. FAU - Cheng, Zhong AU - Cheng Z AD - National Center (Sichuan) of Technology Innovation for Advanced Aviation Equipment Corporation, Chengdu 610073, China. FAU - Zhao, Zheyu AU - Zhao Z AD - Key Laboratory of Multi-Spectral Absorbing Materials and Structures of Ministry of Education, National Engineering Research Center of Electromagnetic Radiation Control Materials, University of Electronic Science and Technology of China, Chengdu 611731, China. FAU - Nangong, Junyi AU - Nangong J AD - Key Laboratory of Multi-Spectral Absorbing Materials and Structures of Ministry of Education, National Engineering Research Center of Electromagnetic Radiation Control Materials, University of Electronic Science and Technology of China, Chengdu 611731, China. FAU - Han, Tiancheng AU - Han T AD - Key Laboratory of Multi-Spectral Absorbing Materials and Structures of Ministry of Education, National Engineering Research Center of Electromagnetic Radiation Control Materials, University of Electronic Science and Technology of China, Chengdu 611731, China. LA - eng GR - National Center of Technology Innovation for Advanced Aviation Equipment Science Foundation/ PT - Journal Article DEP - 20260924 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707293 OTO - NOTNLM OT - broadband microwave absorption OT - electromagnetic-thermal coupling OT - frequency selective surface OT - periodic resistive film OT - thermal management COIS- Authors Wenjun Yu and Dong Yang were employed by Chengdu Aircraft Industrial (Group) Co., Ltd. Author Zhong Cheng was employed by National Center (Sichuan) of Technology Innovation for Advanced Aviation Equipment Corporation. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:46 PHST- 2026/09/05 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - mi17101113 [pii] AID - micromachines-17-01113 [pii] AID - 10.3390/mi17101113 [doi] PST - epublish SO - Micromachines (Basel). 2026 Sep 24;17(10):1113. doi: 10.3390/mi17101113. PMID- 42943865 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Sep 22 TI - Pareto-Guided Process Optimization of Single-Crystal InP Chemical Mechanical Polishing: Balancing Material Removal Rate and Surface Roughness. LID - 10.3390/mi17101108 [doi] LID - 1108 AB - Single-crystal indium phosphide (InP) is an important substrate material for high-speed electronic and optoelectronic devices. Its relatively low hardness, brittleness, and sensitivity to polishing chemistry make it challenging to achieve both efficient material removal and low surface roughness during chemical mechanical polishing (CMP). In this study, response surface methodology (RSM) was combined with multi-objective particle swarm optimization and the Technique for Order Preference by Similarity to Ideal Solution (MOPSO-TOPSIS) to investigate the balance between material removal rate (MRR) and surface roughness (Ra). A three-factor Box-Behnken design was used to establish quadratic models incorporating H(2)O(2) concentration, SiO(2) abrasive concentration, and polishing speed. The models gave coefficients of determination of 0.9898 for MRR and 0.9894 for Ra. Abrasive concentration had the strongest influence on both responses within the investigated ranges. Higher abrasive concentrations favored material removal, whereas lower roughness was obtained at intermediate concentrations and polishing speeds. A compromise condition of 9.79 wt% H(2)O(2), 1.08 wt% SiO(2), and 54.33 rpm was evaluated experimentally. The predicted MRR and Ra were 3871.32 nm/h and 0.6747 nm, respectively, compared with measured values of 4283.79 nm/h and 0.712 nm. The corresponding relative deviations were 10.65% and 5.53%. These results provide a quantitative basis for selecting CMP parameters that balance removal efficiency and surface roughness in single-crystal InP polishing. FAU - Deng, Jiayun AU - Deng J AD - Yunnan Lincang Xinyuan Germanium Industry Co., Ltd., Lincang 677000, China. AD - Faculty of Mechanical and Electrical Engineering, Kunming University of Science and Technology, Kunming 650500, China. AD - Yunnan Xinyao Semiconductor Materials Co., Ltd., Kunming 650503, China. FAU - Zhou, Junjiang AU - Zhou J AD - Faculty of Mechanical and Electrical Engineering, Kunming University of Science and Technology, Kunming 650500, China. FAU - Geng, Jiacheng AU - Geng J AD - Faculty of Mechanical and Electrical Engineering, Kunming University of Science and Technology, Kunming 650500, China. FAU - Wei, Hua AU - Wei H AD - Yunnan Xinyao Semiconductor Materials Co., Ltd., Kunming 650503, China. AD - School of Metallurgy and Energy Engineering, Kunming University of Science and Technology, Kunming 650500, China. FAU - Liu, Hanbao AU - Liu H AD - Yunnan Xinyao Semiconductor Materials Co., Ltd., Kunming 650503, China. FAU - Li, Jiao AU - Li J AD - Yunnan Zhongke Xinyuan Crystal Materials Co., Ltd., Kunming 650503, China. FAU - Wang, Shunjin AU - Wang S AD - Yunnan Xinyao Semiconductor Materials Co., Ltd., Kunming 650503, China. FAU - Li, Changlin AU - Li C AD - Yunnan Zhongke Xinyuan Crystal Materials Co., Ltd., Kunming 650503, China. FAU - Liu, Tinglong AU - Liu T AD - Yunnan Xinyao Semiconductor Materials Co., Ltd., Kunming 650503, China. FAU - Hui, Feng AU - Hui F AD - Yunnan Lincang Xinyuan Germanium Industry Co., Ltd., Lincang 677000, China. AD - Yunnan Xinyao Semiconductor Materials Co., Ltd., Kunming 650503, China. LA - eng GR - 62564008/National Natural Science Foundation of China/ GR - 202301BE070001-030/Yunnan Fundamental Research Projects/ GR - 202401CF070088/Yunnan Fundamental Research Projects/ PT - Journal Article DEP - 20260922 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13706930 OTO - NOTNLM OT - chemical mechanical polishing OT - multi-objective optimization OT - regression analysis OT - response surface methodology OT - single-crystal InP COIS- Author Jiayun Deng is a faculty member at Faculty of Mechanical and Electrical Engineering, Kunming University of Science and Technology. He is also a corporate-sponsored postdoctoral researcher at Yunnan Lincang Xinyuan Germanium Industry Co., Ltd., and serves as the Deputy Chief Technology Officer of Yunnan Xinyao Semiconductor Materials Co., Ltd. Authors Junjiang Zhou and Jiacheng Geng are graduate students (master's candidate) at Faculty of Mechanical and Electrical Engineering, Kunming University of Science and Technology. Author Hua Wei was employed by the company of Yunnan Xinyao Semiconductor Materials Co., Ltd, while also pursuing his doctoral degree at School of Metallurgy and Energy Engineering, Kunming University of Science and Technology. Authors Hanbao Liu, Shunjin Wang and Tinglong Liu was employed by the company of Yunnan Xinyao Semiconductor Materials Co., Ltd. Author Feng Hui was employed by the company of Yunnan Lincang Xinyuan Germanium Industry Co., Ltd. and Yunnan Xinyao Semiconductor Materials Co., Ltd. Authors Jiao Li and Changlin Li was employed by the company of Yunnan Zhongke Xinyuan Crystal Materials Co., Ltd. All authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:46 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - mi17101108 [pii] AID - micromachines-17-01108 [pii] AID - 10.3390/mi17101108 [doi] PST - epublish SO - Micromachines (Basel). 2026 Sep 22;17(10):1108. doi: 10.3390/mi17101108. PMID- 42943864 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Sep 22 TI - Atomistic Insights into Oxidation Mechanisms in Chemical-Mechanical Polishing of Diamond. LID - 10.3390/mi17101107 [doi] LID - 1107 AB - Understanding the interfacial oxidation behavior during chemical-mechanical polishing (CMP) of diamond is important for controlling surface chemical modification and subsequent material removal. In this study, reactive force field molecular dynamics (ReaxFF MD) simulations were employed to comparatively investigate the atomistic evolution of interfacial oxidation, charge transfer, chemical species, and bond structures of single-crystal diamond in *OH- and H(2)O(2)-containing environments under different pressure conditions. The results show that oxidant chemistry and mechanical loading jointly regulate the kinetics and pathway selectivity of interfacial reactions. Elevated pressure promotes early-stage interfacial contact, surface activation, and oxidant consumption; however, the subsequent evolution of C-O bonds is also governed by oxidant-specific reaction kinetics and the availability of reactive species. In the *OH system, highly reactive radicals interact directly with mechanically activated carbon sites, whereas oxidation in the H(2)O(2) system is additionally constrained by the stepwise decomposition of H(2)O(2) and the generation of reactive intermediates, resulting in a comparatively moderated and decomposition-limited oxidation pathway. The interfacial reactions involve the formation and dynamic evolution of C-O, C-OH, and C-H structures, reflecting the competition between oxidation and hydrogenation. XPS measurements after H(2)O(2)-based CMP provide qualitative experimental evidence for the formation of oxygen-containing functional groups on the diamond surface, supporting the occurrence of interfacial oxidation. These findings provide atomistic insight into the coupled effects of oxidant chemistry and mechanical loading during diamond CMP and may assist in the rational design of oxidation-assisted polishing processes. FAU - Wang, Kechong AU - Wang K AUID- ORCID: 0009-0006-9027-3484 AD - School of Mechatronics Engineering, Harbin Institute of Technology, Harbin 150001, China. FAU - Wang, Yunkai AU - Wang Y AD - School of Mechatronics Engineering, Harbin Institute of Technology, Harbin 150001, China. FAU - Li, Meng AU - Li M AD - School of Mechatronics Engineering, Harbin Institute of Technology, Harbin 150001, China. FAU - Li, Xiang AU - Li X AD - School of Mechatronics Engineering, Harbin Institute of Technology, Harbin 150001, China. FAU - Wu, Chongjun AU - Wu C AUID- ORCID: 0000-0001-7529-2747 AD - School of Mechanical Engineering, Donghua University, Shanghai 201620, China. FAU - Hao, Suye AU - Hao S AD - State Key Laboratory for High Performance Tools, Zhengzhou Research Institute for Abrasives & Grinding Co., Ltd., Zhengzhou 450001, China. FAU - Wang, Ningchang AU - Wang N AD - State Key Laboratory for High Performance Tools, Zhengzhou Research Institute for Abrasives & Grinding Co., Ltd., Zhengzhou 450001, China. FAU - Li, Chen AU - Li C AUID- ORCID: 0000-0002-8259-0731 AD - School of Mechatronics Engineering, Harbin Institute of Technology, Harbin 150001, China. LA - eng GR - 52375420/National Natural Science Foundation of China/ GR - CJGJZD20230724093300001/Shenzhen Science and Technology Innovation Commission/ GR - YQ2023E014/Natural Science Foundation of Heilongjiang Province of China/ GR - GXNGJSKL-2024-04/Open Fund of State Key Laboratory for High Performance Tools/ PT - Journal Article DEP - 20260922 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13707766 OTO - NOTNLM OT - ReaxFF MD OT - chemical-mechanical polishing OT - diamond OT - oxidation reaction COIS- Authors Suye Hao and Ningchang Wang were employed by the company Zhengzhou Research Institute for Abrasives & Grinding Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:46 PHST- 2026/08/17 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - mi17101107 [pii] AID - micromachines-17-01107 [pii] AID - 10.3390/mi17101107 [doi] PST - epublish SO - Micromachines (Basel). 2026 Sep 22;17(10):1107. doi: 10.3390/mi17101107. PMID- 42943862 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-666X (Print) IS - 2072-666X (Electronic) IS - 2072-666X (Linking) VI - 17 IP - 10 DP - 2026 Sep 22 TI - A Millimeter-Wave Dual-Band Substrate Integrated Waveguide Filter with Complementary Split Ring Resonators. LID - 10.3390/mi17101105 [doi] LID - 1105 AB - This paper presents a millimeter-wave dual-band bandpass filter based on a substrate integrated waveguide (SIW). The dual-band response is realized by embedding two pairs of complementary split-ring resonators (CSRRs) with different dimensions in the SIW cavity. Their geometry-dependent resonances introduce frequency-selective transmission zeros, reshaping the inherent high-pass response of the SIW into two passbands. The effects of the CSRR dimensions and loading sequence are investigated using several resonator configurations to clarify the formation and frequency control of the two bands. A prototype is fabricated using a wafer-level packaging process. The measured passbands are centered at 54.2 and 87.3 GHz, with insertion losses of 2.69 and 2.83 dB and fractional bandwidths of 21.8% and 13.3%, respectively. The measured results agree well with the simulations. The filter has a compact footprint of (0.63lambdagx0.29lambdag). FAU - Wang, Yanling AU - Wang Y AD - School of Software, Northwestern Polytechnical University, Xi'an 710129, China. AD - Advanced Electronic Systems Laboratory, Beijing Institute of Aerospace Microsystem and Information Technology, Beijing 100094, China. FAU - Zhang, Xinrui AU - Zhang X AD - Microsystems R&D Center, Xi'an Microelectronics Technology Institute, Xi'an 710065, China. FAU - Su, Guodong AU - Su G AUID- ORCID: 0000-0001-6927-9183 AD - Zhejiang Provincial Laboratory of Integrated Circuit Design, Hangzhou Dianzi University, Hangzhou 310018, China. FAU - Wang, Jincheng AU - Wang J AD - Zhejiang Provincial Laboratory of Integrated Circuit Design, Hangzhou Dianzi University, Hangzhou 310018, China. FAU - Liu, Jun AU - Liu J AD - Zhejiang Provincial Laboratory of Integrated Circuit Design, Hangzhou Dianzi University, Hangzhou 310018, China. FAU - Zhang, Kailong AU - Zhang K AUID- ORCID: 0000-0001-8068-3304 AD - School of Software, Northwestern Polytechnical University, Xi'an 710129, China. LA - eng PT - Journal Article DEP - 20260922 PL - Switzerland TA - Micromachines (Basel) JT - Micromachines JID - 101640903 PMC - PMC13706755 OTO - NOTNLM OT - complementary split ring resonator (CSRR) OT - dual-band filter OT - millimeter waves OT - substrate integrated waveguide (SIW) COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:46 PHST- 2026/07/20 00:00 [received] PHST- 2026/09/10 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - mi17101105 [pii] AID - micromachines-17-01105 [pii] AID - 10.3390/mi17101105 [doi] PST - epublish SO - Micromachines (Basel). 2026 Sep 22;17(10):1105. doi: 10.3390/mi17101105. PMID- 42943854 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 21 TI - Prunus mume Extract Cooperates with Cisplatin to Induce Melanoma Apoptosis and Regulate Immune Responses. LID - 10.3390/molecules31203786 [doi] LID - 3786 AB - This study investigated the potential of Prunus mume (P. mume) extract as an anticancer adjuvant by evaluating its direct apoptotic effects on A375P human melanoma cells and its immunomodulatory impact on peripheral blood mononuclear cells (PBMCs). Furthermore, the extract demonstrated additive and chemosensitizing effects when combined with a sub-toxic dose of cisplatin, significantly enhancing the growth inhibition of A375P cells. Selective growth inhibition was confirmed using crystal violet assays, where the 80% ethanol (EtOH) extract exhibited significant dose-dependent inhibition of A375P viability while leaving human adult low-calcium high-temperature keratinocytes (HaCaT) largely unaffected. Apoptotic cell death was characterized by DNA fragmentation and disrupted mitochondrial membrane potential, which decreased to 77.3% of the control as evidenced by JC-1 staining. At the molecular level, quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) analysis demonstrated the upregulation of pro-apoptotic markers, including Bax (2.08-fold at 30 h) and Caspase-3 (2.12-fold in 16 h floating cells), alongside significant downregulation of the anti-apoptotic gene Bcl-2 (down to 0.10-fold at 16 h adherent cells). Notably, initiator Caspase-9 expression was suppressed in co-treated cells, suggesting late-stage transcriptional attenuation during sustained drug exposure. The extract suppressed the NF-kappaB pathway and reduced PD-L1 mRNA levels by 2.63-fold (compared to 3.55-fold with cisplatin alone), suggesting a mechanism to mitigate chemo-induced inflammatory stress and immune dysregulation. Furthermore, while cisplatin induced an excessive inflammatory response in PBMCs, co-treatment with the extract normalized the cytokine profile by modulating IFN-gamma (2.21-fold), TNF-alpha (3.37-fold), and IL-2 (1.60-fold) back toward baseline levels. The attenuation of cytotoxic effectors, Granzyme B (1.16-fold) and Perforin (0.73-fold), along with the costimulatory molecule CD80 (4.44-fold), suggests the modulation of excessive inflammatory activation and attenuation of immune hyper-responsiveness. Finally, biomolecular docking predictions using the Boltz-2 framework (an open-source biomolecular interaction prediction model) demonstrated high-affinity binding between key plum constituents (e.g., chlorogenic acid, citric acid) and target proteins such as Caspase-3, Bcl-2, Nrf2, and Keap1, providing a structural basis for their bioactivity. In conclusion, P. mume extract (PME) serves as a promising multi-target adjuvant that simultaneously triggers cancer cell apoptosis while mitigating cisplatin-induced inflammatory perturbations in immune cells. FAU - Kim, Je Hyoung AU - Kim JH AD - Jeonnam Institute of Health and Environment, Muan-gun, Jeonnam-Gwangju Special Metropolitan City 58568, Republic of Korea. FAU - Oh, Gyu Yeon AU - Oh GY AD - Jeonnam Institute of Health and Environment, Muan-gun, Jeonnam-Gwangju Special Metropolitan City 58568, Republic of Korea. FAU - Kim, Eun Hee AU - Kim EH AD - Jeonnam Institute of Health and Environment, Muan-gun, Jeonnam-Gwangju Special Metropolitan City 58568, Republic of Korea. FAU - Lee, Hee Yeon AU - Lee HY AD - Jeonnam Institute of Health and Environment, Muan-gun, Jeonnam-Gwangju Special Metropolitan City 58568, Republic of Korea. FAU - Yang, Eun Jin AU - Yang EJ AD - Jeonnam Institute of Health and Environment, Muan-gun, Jeonnam-Gwangju Special Metropolitan City 58568, Republic of Korea. FAU - Park, Hye Young AU - Park HY AD - Jeonnam Institute of Health and Environment, Muan-gun, Jeonnam-Gwangju Special Metropolitan City 58568, Republic of Korea. FAU - Park, Sook AU - Park S AD - Jeonnam Institute of Health and Environment, Muan-gun, Jeonnam-Gwangju Special Metropolitan City 58568, Republic of Korea. FAU - An, Yang Jun AU - An YJ AD - Jeonnam Institute of Health and Environment, Muan-gun, Jeonnam-Gwangju Special Metropolitan City 58568, Republic of Korea. FAU - Kim, Jin Young AU - Kim JY AD - Jeonnam Institute of Health and Environment, Muan-gun, Jeonnam-Gwangju Special Metropolitan City 58568, Republic of Korea. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - Q20Q21Q62J (Cisplatin) RN - 0 (Plant Extracts) RN - 0 (Antineoplastic Agents) SB - IM MH - Humans MH - *Cisplatin/pharmacology MH - *Apoptosis/drug effects MH - *Plant Extracts/pharmacology/chemistry MH - *Melanoma/drug therapy/immunology/pathology/metabolism MH - *Prunus/chemistry MH - Cell Line, Tumor MH - Membrane Potential, Mitochondrial/drug effects MH - Leukocytes, Mononuclear/drug effects/immunology/metabolism MH - Cell Survival/drug effects MH - Antineoplastic Agents/pharmacology PMC - PMC13707659 OTO - NOTNLM OT - Prunus mume OT - apoptosis OT - cancer adjuvant OT - immune activation OT - melanoma OT - mitochondrial pathway COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/21 CRDT- 2026/10/27 10:46 PHST- 2026/10/06 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - molecules31203786 [pii] AID - molecules-31-03786 [pii] AID - 10.3390/molecules31203786 [doi] PST - epublish SO - Molecules. 2026 Oct 21;31(20):3786. doi: 10.3390/molecules31203786. PMID- 42943852 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 21 TI - Interactions Between Cadmium Contamination and Mineral Sorbents in Shaping Heavy Metal Bioaccumulation in Soil and Plant Biomass. LID - 10.3390/molecules31203784 [doi] LID - 3784 AB - By disrupting soil ecosystem functioning, cadmium threatens food safety. Therefore, a significant research imperative is to develop effective remediation strategies and understand the relationships between cadmium content in soil and plants and the content of other metals. This study aimed to evaluate the effects of calcium carbonate, sepiolite, expanded clay, zeolite, and molecular sieve on the growth and physiological response of Zea mays grown in cadmium-contaminated soil. Plant growth and development were assessed together with the translocation and bioaccumulation of cadmium and other metals (Pb, Cr, Cu, Zn, Fe, Ni, and Mn) and changes in soil physicochemical properties. The study was conducted as a pot experiment using uncontaminated soil and soil contaminated with 15 and 30 mg Cd kg(-1). Depending on the contamination level, cadmium reduced the leaf greenness index of Zea mays by 10-16%, the yield of the aboveground parts by 60-76%, and root yield by 60-69%. The tested sorbents modified these responses mainly by altering the soil physicochemical properties. The molecular sieve was the most effective, reducing the negative effect of cadmium on the yield of the aboveground parts and roots by an average of 31% while not significantly affecting the leaf greenness index. Cadmium increased the translocation factor of all analyzed elements except cadmium itself, indicating its potential to affect the homeostasis of other elements in the plant. Heavy metal bioaccumulation in the roots of Zea mays was significantly greater than in the aboveground parts. Cadmium altered the bioaccumulation of individual elements to different extents, but these effects could be modified through the appropriate selection of mineral sorbents. These findings make an important contribution to the development of effective methods for remediating cadmium-contaminated soils. FAU - Wyszkowska, Jadwiga AU - Wyszkowska J AUID- ORCID: 0000-0002-2156-3780 AD - Department of Soil Science and Microbiology, Faculty of Agriculture and Forestry, University of Warmia and Mazury in Olsztyn, Plac Lodzki 3, 10-727 Olsztyn, Poland. FAU - Borowik, Agata AU - Borowik A AUID- ORCID: 0000-0002-8512-7363 AD - Department of Soil Science and Microbiology, Faculty of Agriculture and Forestry, University of Warmia and Mazury in Olsztyn, Plac Lodzki 3, 10-727 Olsztyn, Poland. FAU - Zaborowska, Magdalena AU - Zaborowska M AUID- ORCID: 0000-0003-0092-2729 AD - Department of Soil Science and Microbiology, Faculty of Agriculture and Forestry, University of Warmia and Mazury in Olsztyn, Plac Lodzki 3, 10-727 Olsztyn, Poland. FAU - Kucharski, Jan AU - Kucharski J AUID- ORCID: 0000-0002-6364-6357 AD - Department of Soil Science and Microbiology, Faculty of Agriculture and Forestry, University of Warmia and Mazury in Olsztyn, Plac Lodzki 3, 10-727 Olsztyn, Poland. LA - eng GR - University of Warmia and Mazury in Olsztyn, Faculty of Agriculture and Forestry, Department of Soil Science and Microbiology (grant No. 30.610.006-110)/University of Warmia and Mazury in Olsztyn/ GR - Minister of Science under the Regional Initiative of Excellence Program/University of Warmia and Mazury in Olsztyn/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 00BH33GNGH (Cadmium) RN - 0 (Soil Pollutants) RN - 0 (Metals, Heavy) RN - 0 (Soil) RN - 0 (Minerals) SB - IM MH - *Cadmium/chemistry/metabolism MH - *Soil Pollutants/chemistry/metabolism MH - *Metals, Heavy/metabolism/chemistry MH - *Zea mays/growth & development/metabolism/drug effects MH - Biomass MH - *Soil/chemistry MH - Bioaccumulation MH - *Minerals/chemistry MH - Plant Roots/metabolism/drug effects MH - Adsorption PMC - PMC13707395 OTO - NOTNLM OT - Zea mays OT - bioaccumulation OT - heavy metals OT - metal uptake OT - remediation OT - sorbents OT - translocation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/21 CRDT- 2026/10/27 10:46 PHST- 2026/09/21 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - molecules31203784 [pii] AID - molecules-31-03784 [pii] AID - 10.3390/molecules31203784 [doi] PST - epublish SO - Molecules. 2026 Oct 21;31(20):3784. doi: 10.3390/molecules31203784. PMID- 42943850 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 21 TI - In Silico Investigation of Candidate Anticancer Targets for Aryltetralin Lignans Previously Reported from Cleistanthus Species. LID - 10.3390/molecules31203781 [doi] LID - 3781 AB - Aryltetralin lignans isolated from the fruits of Cleistanthus species exhibit substantial cytotoxic activity, although the molecular interactions underlying these effects remain incompletely understood. This study combined computational target investigation with previously published experimental chemical characterization and cytotoxic evidence and no new isolation or cytotoxicity experiments were carried out. Cleistantoxin, 7-hydroxypicropolygamain, neo-cleistantoxin, cleisindoside A, cleisindoside D, and cleistonkinin D were previously isolated from C. indochinensis, C. tonkinensis, C. eberhardtii and characterized by complementary spectroscopic methods. Cleistantoxin showed potent activity against MCF-7, multidrug-resistant MCF-7R, and HT29 cells, with reported IC(50) values of 0.036, 0.014, and 0.035 muM, respectively. Structural similarity to podophyllotoxin-derived anticancer agents guided the investigation of DNA topoisomerase IIbeta, Bcl-2, and p53 Y220C as candidate targets. Docking with AutoDock Vina-GPU 2.1 was validated by redocking the co-crystallized inhibitors (RMSD < 2 A at the TOP2B and Bcl-2 sites; the p53 Y220C control is reported for both receptor preparations), and selected complexes were simulated in triplicate for 100 ns, followed by MM-GBSA calculations and ADMET predictions. Cleisindoside A and D kept etoposide-like hydrogen bonds to DNA in the TOP2B-DNA complex. Their MM-GBSA binding energies were -78.40 +/- 3.73 and -81.90 +/- 7.84 kcal.mol(-1) for two ligand copies, comparable to -79.62 +/- 5.14 kcal.mol(-1) for etoposide. In Bcl-2, S-10r was clearly more favorable than cleisindoside D. The predicted pharmacokinetic profiles differentiated the aglycones from the glycosides and identified potential cytochrome P450 inhibition liabilities. By linking previously reported experimentally characterized natural products and cytotoxic evidence with molecular modeling, this study provides testable hypotheses for the molecular recognition of Cleistanthus lignans. Direct biochemical and mechanism-focused cellular studies are nevertheless required to validate the proposed targets. FAU - Nguyen, Lam-Hong AU - Nguyen LH AD - Faculty of Analytical Chemistry and Drug Quality Control, Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Hanoi 100000, Vietnam. FAU - Nguyen, Thi-Thanh-Thuy AU - Nguyen TT AD - Faculty of Pharmacy, University of Health Sciences, Vietnam National University Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam. AD - Research Center for Discovery and Development of Healthcare Products, Vietnam National University Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam. FAU - Nguyen, Dac-Nhan AU - Nguyen DN AUID- ORCID: 0000-0001-5521-7790 AD - Faculty of Pharmacy, University of Health Sciences, Vietnam National University Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam. AD - Research Center for Discovery and Development of Healthcare Products, Vietnam National University Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam. FAU - Mai, Quang-Minh AU - Mai QM AUID- ORCID: 0009-0007-8815-9241 AD - Faculty of Pharmacy, University of Health Sciences, Vietnam National University Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam. AD - Research Center for Discovery and Development of Healthcare Products, Vietnam National University Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam. FAU - Le, Thang AU - Le T AUID- ORCID: 0009-0004-3349-3814 AD - Faculty of Pharmacy, University of Health Sciences, Vietnam National University Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam. AD - Research Center for Discovery and Development of Healthcare Products, Vietnam National University Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam. FAU - Pham-The, Hai AU - Pham-The H AUID- ORCID: 0000-0003-4531-7223 AD - Department of Medicine and Pharmacy, University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Hanoi 100000, Vietnam. FAU - Pham, Van-Cuong AU - Pham VC AD - Institute of Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Hanoi 100000, Vietnam. FAU - Doan, Thi-Mai-Huong AU - Doan TM AD - Institute of Chemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Hanoi 100000, Vietnam. FAU - Tran, Viet-Hung AU - Tran VH AUID- ORCID: 0000-0001-8077-1021 AD - Institute of Drug Quality Control at Ho Chi Minh City, 200 Co Bac, Ho Chi Minh City 700000, Vietnam. FAU - Thai, Khac-Minh AU - Thai KM AUID- ORCID: 0000-0002-5279-9614 AD - Faculty of Pharmacy, University of Health Sciences, Vietnam National University Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam. AD - Research Center for Discovery and Development of Healthcare Products, Vietnam National University Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam. LA - eng GR - NCM2024-44-01/Vietnam National University Ho Chi Minh City/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Lignans) RN - 0 (Antineoplastic Agents, Phytogenic) RN - EC 5.99.1.3 (DNA Topoisomerases, Type II) RN - 0 (Proto-Oncogene Proteins c-bcl-2) RN - 0 (Tumor Suppressor Protein p53) SB - IM MH - Humans MH - *Lignans/chemistry/pharmacology MH - Molecular Docking Simulation MH - *Antineoplastic Agents, Phytogenic/pharmacology/chemistry MH - Computer Simulation MH - Cell Line, Tumor MH - MCF-7 Cells MH - DNA Topoisomerases, Type II/chemistry/metabolism MH - Proto-Oncogene Proteins c-bcl-2/chemistry/antagonists & inhibitors/metabolism MH - Tumor Suppressor Protein p53/chemistry/antagonists & inhibitors/metabolism MH - Molecular Structure PMC - PMC13707973 OTO - NOTNLM OT - ADMET OT - Cleistanthus OT - MM-GBSA OT - aryltetralin lignans OT - cleistantoxin OT - molecular docking OT - molecular dynamics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/21 CRDT- 2026/10/27 10:46 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - molecules31203781 [pii] AID - molecules-31-03781 [pii] AID - 10.3390/molecules31203781 [doi] PST - epublish SO - Molecules. 2026 Oct 21;31(20):3781. doi: 10.3390/molecules31203781. PMID- 42943841 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 20 TI - Discrimination of Shellac and Nitrocellulose Varnishes Using Ambient Mass Spectrometry and Partial Least Squares Regression. LID - 10.3390/molecules31203772 [doi] LID - 3772 AB - Historical surface coatings on Czechoslovak furniture from the interwar period (1918-1945) frequently combine shellac and nitrocellulose lacquers, yet their compositional characterisation in aged specimens remains analytically challenging. We report the first application of atmospheric solids analysis probe mass spectrometry (ASAP-MS) combined with partial least squares regression for semiquantitative estimation of shellac/nitrocellulose ratios in historical varnish systems. ATR-FTIR spectroscopy combined with principal component analysis represents a standard tool in conservation practice for varnish characterisation; however, our comparative evaluation demonstrated that this approach is limited to qualitative discrimination and does not support reliable semiquantitative composition estimates. The ASAP-MS workflow was applied to microsamples from an authenticated historical furniture object of Czech origin (ca. 1939), yielding direct compositional estimates that can guide the selection of appropriate restoration treatments and reduce the risk of chemically incompatible interventions. FAU - Dostal, Stepan AU - Dostal S AUID- ORCID: 0009-0005-8230-0262 AD - Department of Analytical Chemistry, Faculty of Science, Palacky University, 17. Listopadu 12, 77900 Olomouc, Czech Republic. FAU - Urban, Vilem AU - Urban V AUID- ORCID: 0000-0002-4331-702X AD - Department of Art History, Faculty of Arts, Palacky University, Univerzitni 3, 77900 Olomouc, Czech Republic. FAU - Kucera, Lukas AU - Kucera L AUID- ORCID: 0000-0002-3773-6439 AD - Department of Analytical Chemistry, Faculty of Science, Palacky University, 17. Listopadu 12, 77900 Olomouc, Czech Republic. LA - eng GR - IGA_PrF_2026_024/Palacky University Olomouc/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 SB - IM PMC - PMC13707626 OTO - NOTNLM OT - cultural heritage OT - infrared spectroscopy OT - lacquers OT - mass spectrometry COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:46 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - molecules31203772 [pii] AID - molecules-31-03772 [pii] AID - 10.3390/molecules31203772 [doi] PST - epublish SO - Molecules. 2026 Oct 20;31(20):3772. doi: 10.3390/molecules31203772. PMID- 42943839 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 20 TI - Boosted H(2)O(2) Photosynthesis via Synergistic Tuning Electronic Structure and Interfacial O(2) Transfer on Floatable Carbon Nitride Networks. LID - 10.3390/molecules31203770 [doi] LID - 3770 AB - Solar-driven photosynthesis of hydrogen peroxide (H(2)O(2)) from O(2) and H(2)O offers an eco-friendly and sustainable route. Metal-free graphitic carbon nitride (CN) is capable of photocatalytic H(2)O(2) production, yet its efficiency is limited by insufficient charge separation and slow O(2) supply in conventional diphase systems. Herein, we graft cinnamylidene groups into CN to fabricate a hydrophobic CN nanolayer (CNCA/CF). The incorporated cinnamylidene groups with extended conjugated chains narrow the bandgap, promote photocarrier separation and improve 2e ORR selectivity. Benefiting from the hydrophobicity of CNCA/CF, it can stably float on water and construct a gas-solid-liquid triphase interface that enables rapid O(2) diffusion and delivery. Moreover, floating CNCA/CF exhibits a high H(2)O(2) output of 1370 mumol m(-2) h(-1) in a homemade reactor, 15.3-fold higher than that of pristine CN. Mechanistic studies demonstrated that H(2)O(2) is generated through a sequential 2e ORR process involving .O(2)(-) as the crucial intermediate. This work highlights a feasible strategy combining triphase interface optimization with electronic tuning, providing a new platform for the rational design of photocatalysts for H(2)O(2) triphase photosynthesis. FAU - Peng, Xiaoying AU - Peng X AD - Research Center of Advanced Materials and Green Energy, Jiangxi Institute of Technology, Nanchang 330098, China. FAU - Jiang, Zhangsong AU - Jiang Z AD - Research Center of Advanced Materials and Green Energy, Jiangxi Institute of Technology, Nanchang 330098, China. FAU - Liu, Fanghua AU - Liu F AD - Research Center of Advanced Materials and Green Energy, Jiangxi Institute of Technology, Nanchang 330098, China. FAU - Xiang, Bingzhi AU - Xiang B AD - Research Center of Advanced Materials and Green Energy, Jiangxi Institute of Technology, Nanchang 330098, China. FAU - Zhu, Jielu AU - Zhu J AD - Research Center of Advanced Materials and Green Energy, Jiangxi Institute of Technology, Nanchang 330098, China. FAU - Lin, Jie AU - Lin J AD - Research Center of Advanced Materials and Green Energy, Jiangxi Institute of Technology, Nanchang 330098, China. FAU - Wang, Bo AU - Wang B AD - Research Center of Advanced Materials and Green Energy, Jiangxi Institute of Technology, Nanchang 330098, China. FAU - Song, Junwei AU - Song J AD - Research Center of Advanced Materials and Green Energy, Jiangxi Institute of Technology, Nanchang 330098, China. FAU - Yang, Kai AU - Yang K AUID- ORCID: 0000-0002-3138-3740 AD - School of Chemistry and Chemical Engineering, Jiangxi University of Science and Technology, Ganzhou 341000, China. FAU - Peng, Guiming AU - Peng G AD - Key Laboratory of Fluorine and Silicon for Energy Materials and Chemistry of Ministry of Education, College of Chemistry and Materials, Jiangxi Normal University, Nanchang 330022, China. LA - eng GR - 22366018/National Natural Science Foundation of China/ GR - GJJ2202624/Education Department of Jiangxi Province/ GR - GJJ2402506/Education Department of Jiangxi Province/ GR - GJJ212022/Education Department of Jiangxi Province/ GR - 20252BAC240238/Jiangxi Provincial Department of Science and Technology/ GR - 20232ACB203022/Jiangxi Provincial Department of Science and Technology/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 SB - IM PMC - PMC13707579 OTO - NOTNLM OT - H2O2 OT - carbon nitride OT - photosynthesis OT - triphase interface COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:46 PHST- 2026/09/28 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - molecules31203770 [pii] AID - molecules-31-03770 [pii] AID - 10.3390/molecules31203770 [doi] PST - epublish SO - Molecules. 2026 Oct 20;31(20):3770. doi: 10.3390/molecules31203770. PMID- 42943835 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 20 TI - QSAR Modeling and Fragment Contribution Analysis of N-Benzyltryptamines at Human 5-HT2 Receptor Subtypes. LID - 10.3390/molecules31203764 [doi] LID - 3764 AB - Serotonin 5-HT2 receptor agonists are returning to psychiatric drug development, but separating the three receptor subtypes pharmacologically remains difficult, and agonism at 5-HT2B carries a risk of valvular heart disease. A recent study reported affinities of 46 N-benzyltryptamines and N-benzyl-5-methoxytryptamines at all three human subtypes and analyzed them with an additive substituent model, which gave fragment coefficients of the same sign at every subtype and suggested that selectivity within this scaffold would be hard to achieve. We revisited that conclusion with non-additive models built under a protocol designed for small datasets: fifty stratified partitions with all filtering and hyperparameter tuning inside each training set, external statistics reported as distributions, response permutation on three partitions per endpoint, and partitions that withhold an entire scaffold or an entire substitution position. We repeated the analysis with two descriptor sets that share no variables and are built on different principles. Penalized linear models were predictive and transferable, whereas gradient boosting reproduced the training set exactly, and neither tree ensemble transferred to a held-out scaffold. Fragment contributions at 5-HT2A and 5-HT2C correlated at 0.25 compared with 0.77 for whole-molecule affinities, and both descriptor sets gave the same figure within 0.01. All models failed on para-substituted analogs, which distance-based applicability-domain criteria did not flag, whereas models trained without the ortho or the meta block stayed above a mean-predictor baseline. Aggregation over the whole molecule, rather than descriptor choice, conceals subtype divergence. One group drives the divergence: the N-benzyl substituent raises the predicted 5-HT2A affinity and lowers the predicted 5-HT2C affinity; excluding it raised the correlation to 0.93. FAU - Dordevic, Vladimir AU - Dordevic V AD - Department of Psychiatry with Medical Psychology, Faculty of Medicine, University of Nis, 18000 Nis, Serbia. FAU - Binic, Iva AU - Binic I AUID- ORCID: 0000-0002-6457-990X AD - Clinic of Psychiatry, University Clinical Center Nis, 18000 Nis, Serbia. FAU - Petrovic, Jovana AU - Petrovic J AD - Clinic of Psychiatry, University Clinical Center Nis, 18000 Nis, Serbia. FAU - Stojanov, Jelena AU - Stojanov J AUID- ORCID: 0000-0003-1959-7890 AD - Center for Mental Health Protection, University Clinical Center Nis, 18000 Nis, Serbia. FAU - Mitic, Zarko AU - Mitic Z AD - Department of Chemistry, Faculty of Medicine, University of Nis, 18000 Nis, Serbia. FAU - Veselinovic, Aleksandar M AU - Veselinovic AM AUID- ORCID: 0000-0001-9291-6654 AD - Department of Chemistry, Faculty of Medicine, University of Nis, 18000 Nis, Serbia. LA - eng GR - 451-03-34/2026-03/200113/Ministry of Science, Technological Development, and Innovation/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Serotonin 5-HT2 Receptor Agonists) RN - 0 (Receptors, Serotonin, 5-HT2) RN - 0 (Receptor, Serotonin, 5-HT2A) RN - 0 (Receptor, Serotonin, 5-HT2B) RN - 0 (Tryptamines) SB - IM MH - Humans MH - *Quantitative Structure-Activity Relationship MH - *Serotonin 5-HT2 Receptor Agonists/chemistry/pharmacology MH - Models, Molecular MH - *Receptors, Serotonin, 5-HT2/chemistry/metabolism MH - Receptor, Serotonin, 5-HT2A/chemistry MH - Receptor, Serotonin, 5-HT2B/chemistry MH - *Tryptamines/chemistry/pharmacology PMC - PMC13707879 OTO - NOTNLM OT - 5-HT2 receptors OT - N-benzyltryptamines OT - applicability domain OT - model interpretation OT - non-additivity OT - quantitative structure-activity relationships OT - simplex descriptors OT - subtype selectivity COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/20 CRDT- 2026/10/27 10:46 PHST- 2026/09/08 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - molecules31203764 [pii] AID - molecules-31-03764 [pii] AID - 10.3390/molecules31203764 [doi] PST - epublish SO - Molecules. 2026 Oct 20;31(20):3764. doi: 10.3390/molecules31203764. PMID- 42943828 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 19 TI - Synthesis and sigma Receptor Affinity of 2-(1,3-Dioxan-4-yl)ethanamines with Axially Oriented Phenyl Moiety. LID - 10.3390/molecules31203759 [doi] LID - 3759 AB - 2-(1,3-Dioxan-4-yl)ethanamines 6 and 7 display high affinity towards the PCP binding site of NMDA receptors and sigma(1) receptors depending on the substitution pattern in 2-position of the 1,3-dioxane ring. Herein, conformationally restricted 2-(1,3-dioxan-4-yl)ethan-amines with the phenyl ring embedded in tricyclic systems were designed, synthesized and pharmacologically evaluated. The first key step of the synthesis was the nucleophilic addition of phenyllithium derivatives bearing various acetalic substituents in o-position at pentan-3-one 9. Subsequent double intramolecular transacetalization of the resulting tertiary alcohols 11, 18 and 25 provided the tricyclic alcohols 14, 21 and 28, allowing the introduction of various amino groups. Primary amines (a-compounds) showed either very weak (23a: K(i) = 537 nM) or no affinity towards the PCP binding site of the NMDA receptor. However, benzylamines (b-compounds) and the corresponding hydrogenated cyclohexylmethylamines (d-compounds) revealed high sigma(1) affinity. The highest sigma(1) affinity was obtained for the cyclohexylmethylamines 30d (K(i) = 7.1 nM) and 30k (K(i) = 7.4 nM). Due to the additional N-methyl moiety, tertiary amine 30k showed higher lipophilicity (logD(7.4) = 1.7) than secondary amine 30d (logD(7.4) = 1.0) resulting in a higher LLE value for 30d (LLE = 7.2) than for 30k (LLE = 6.4). Whereas cyclohexylmethyl-substituted amines 8d, 16d, 30d and 30k showed only moderate metabolic stability (less than 42% parent compound after 90 min), analogous benzylamines 8b, 16b and 30b are considerably more stable (>/=89% intact parent after 90 min). Benzyl- and cyclohexylmethylamines of all three series of ligands reveal intermediate human serum albumin binding between 63% and 77%. FAU - Schulte-Gauczinski, Nina AU - Schulte-Gauczinski N AD - Institut fur Pharmazeutische und Medizinische Chemie, Universitat Munster, Corrensstrasse 48, D 48149 Munster, Germany. FAU - Schmidt, Judith AU - Schmidt J AD - Institut fur Pharmazeutische und Medizinische Chemie, Universitat Munster, Corrensstrasse 48, D 48149 Munster, Germany. FAU - Frehland, Bastian AU - Frehland B AD - Institut fur Pharmazeutische und Medizinische Chemie, Universitat Munster, Corrensstrasse 48, D 48149 Munster, Germany. FAU - Schepmann, Dirk AU - Schepmann D AD - Institut fur Pharmazeutische und Medizinische Chemie, Universitat Munster, Corrensstrasse 48, D 48149 Munster, Germany. FAU - Daniliuc, Constantin AU - Daniliuc C AUID- ORCID: 0000-0002-6709-3673 AD - Organisch-Chemisches Institut, Universitat Munster, Corrensstrasse 40, D 48149 Munster, Germany. FAU - Wunsch, Bernhard AU - Wunsch B AUID- ORCID: 0000-0002-9030-8417 AD - Institut fur Pharmazeutische und Medizinische Chemie, Universitat Munster, Corrensstrasse 48, D 48149 Munster, Germany. LA - eng GR - WU176/Deutsche Forschungsgemeinschaft/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Receptors, sigma) RN - 0 (Receptors, N-Methyl-D-Aspartate) RN - 0 (Dioxanes) SB - IM MH - *Receptors, sigma/metabolism/chemistry MH - Binding Sites MH - Receptors, N-Methyl-D-Aspartate/metabolism/chemistry MH - Humans MH - *Dioxanes/chemistry/chemical synthesis MH - Molecular Structure MH - Protein Binding PMC - PMC13707864 OTO - NOTNLM OT - 2,4-disubstituted 1,3-dioxanes OT - 2-substituted ethan-1-amines OT - conformational restriction OT - human serum albumin binding OT - intramolecular transacetalization OT - ligand-lipophilicity efficiency (LLE) OT - metabolic stability OT - phencyclidine (PCP) binding site of the NMDA receptor, sigma1 receptor OT - receptor affinity OT - selectivity OT - structure affinity relationships OT - synthesis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/19 CRDT- 2026/10/27 10:46 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - molecules31203759 [pii] AID - molecules-31-03759 [pii] AID - 10.3390/molecules31203759 [doi] PST - epublish SO - Molecules. 2026 Oct 19;31(20):3759. doi: 10.3390/molecules31203759. PMID- 42943827 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 19 TI - Investigation of Potential Mechanisms of Yerba Santa Compounds Against Alzheimer's Disease. LID - 10.3390/molecules31203757 [doi] LID - 3757 AB - Alzheimer's disease (AD) is a progressive neurodegenerative disorder in which chronic neuroinflammation plays a central role in disease initiation and progression. Yerba Santa (Eriodictyon spp.) is a medicinal plant with reported anti-inflammatory and neuroprotective properties, although its molecular mechanisms remain poorly understood. This study investigated whether chemically characterized Yerba Santa compounds show predicted docking interactions with neuroinflammation-related protein targets implicated in AD. Thirty-six ligand structures, representing 30 mass spectrometry peak assignments, were docked against 30 neuroinflammation-related proteins using the MolSoft ICM molecular docking platform. To improve reproducibility, each ligand-target pair was evaluated by replicated docking simulations followed by statistical analysis, with apo and holo targets evaluated against separate statistical references and expanded docking where applicable. Under the apo -25 kcal/mol screening reference, 11 ligand-target pairs were retained after BH-FDR correction and expanded docking for the apo C1r-C1s (PDB:6F39). Separately, 23 holo ligand-target pairs were retained after comparison with their corresponding redocked native-ligand references: Complement C1q (PDB:2JG8), GABA-A receptor alpha1beta2gamma2 (PDB:6D6U), and NLRP3 (PDB:7PZC). The pairs retained under these separate criteria involved multiple Yerba Santa compounds, predominantly flavonoids, while Rosmarinic acid (L21) was retained for both Complement C1q (PDB:2JG8) and NLRP3 (PDB:7PZC). These findings support a selective predicted multi-target interaction profile for Yerba Santa compounds and identify complement signaling, GABA-A receptor-associated neurotransmission, and NLRP3 inflammasome signaling as testable pathways for future experimental studies of neuroinflammation in AD. FAU - Arevalo Navarro, Magally AU - Arevalo Navarro M AD - Department of Chemical and Biological Engineering, Villanova University, Villanova, PA 19085, USA. FAU - Alfano, Charles AU - Alfano C AD - Department of Chemical and Biological Engineering, Villanova University, Villanova, PA 19085, USA. FAU - Cross, Jonathan AU - Cross J AD - Department of Chemical and Biological Engineering, Villanova University, Villanova, PA 19085, USA. FAU - Zhai, Tianhua AU - Zhai T AUID- ORCID: 0009-0007-9712-9132 AD - Department of Biostatistics, Epidemiology and Informatics, University of Pennsylvania, Philadelphia, PA 19104, USA. FAU - Shen, Li AU - Shen L AUID- ORCID: 0000-0002-5443-0503 AD - Department of Biostatistics, Epidemiology and Informatics, University of Pennsylvania, Philadelphia, PA 19104, USA. FAU - Huang, Zuyi AU - Huang Z AUID- ORCID: 0000-0002-1393-2961 AD - Department of Chemical and Biological Engineering, Villanova University, Villanova, PA 19085, USA. LA - eng GR - U01 AG066833/National Institutes of Health (NIH) - National Institute on Aging (NIA)/ GR - R01 AG071470/National Institutes of Health (NIH) - National Institute on Aging (NIA)/ GR - U19 AG074879/AG/NIA NIH HHS/United States GR - U01 AG068057/AG/NIA NIH HHS/United States GR - U01 AG068057/National Institutes of Health (NIH) - National Institute on Aging (NIA)/ GR - U01 AG066833/AG/NIA NIH HHS/United States GR - R01 AG071470/AG/NIA NIH HHS/United States GR - U19 AG074879/National Institutes of Health (NIH) - National Institute on Aging (NIA)/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Ligands) RN - 0 (Plant Extracts) RN - 0 (Neuroprotective Agents) SB - IM MH - *Alzheimer Disease/drug therapy/metabolism MH - Molecular Docking Simulation MH - Humans MH - Ligands MH - *Plant Extracts/chemistry/pharmacology MH - *Neuroprotective Agents/chemistry/pharmacology PMC - PMC13707469 OTO - NOTNLM OT - Alzheimer's disease OT - Eriodictyon OT - Yerba Santa OT - complement system OT - flavonoids OT - molecular docking OT - natural products OT - neuroinflammation OT - statistical validation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/19 CRDT- 2026/10/27 10:46 PHST- 2026/08/10 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - molecules31203757 [pii] AID - molecules-31-03757 [pii] AID - 10.3390/molecules31203757 [doi] PST - epublish SO - Molecules. 2026 Oct 19;31(20):3757. doi: 10.3390/molecules31203757. PMID- 42943812 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 18 TI - The Effect of Organic Inhibitors on the Corrosion of Mild Steel in Varying Crude Oil-Brine Emulsions: The Electrochemical Protocol Selection. LID - 10.3390/molecules31203743 [doi] LID - 3743 AB - Corrosion inhibitors are widely used in oil production, transportation, and refining, where crude oil-brine emulsions complicate electrochemical testing because of their heterogeneity and low conductivity. This study examined the use of Tafel polarization and electrochemical impedance spectroscopy to assess mild steel corrosion in brine and crude oil-brine emulsions in the presence of Nalco EC 1010B and Scimol OR 2001. Emulsions with a continuous conductive phase were tested directly, whereas the brine phase of reverse emulsions was separated before measurement. In brine, both inhibitors provided more than 80% protection at a concentration of 20 mg/L. At this concentration, corrosion rates in the inhibited direct emulsion and the brine phase separated from the reverse emulsion were approximately 0.20-0.25 mm/year, comparable to those in inhibited brine under similar conditions. In stable emulsions, the surfactant also affected steel corrosion, complicating the assessment of inhibitor performance. These results indicate that measurements in brine can guide the selection of inhibitor concentrations for direct and reverse emulsions under the conditions investigated. FAU - Ivanova, Nataliya A AU - Ivanova NA AUID- ORCID: 0000-0002-3533-4081 AD - D.V. Sokolsky Institute of Fuel, Catalysis and Electrochemistry JSC, 142 Kunayev Str., Almaty 050010, Kazakhstan. AD - Al-Farabi Kazakh National University, 71, Al-Farabi Avenue, Almaty 050012, Kazakhstan. FAU - Alibayeva, Adina AU - Alibayeva A AD - D.V. Sokolsky Institute of Fuel, Catalysis and Electrochemistry JSC, 142 Kunayev Str., Almaty 050010, Kazakhstan. AD - Kazakh British Technical University, Tole bi Street, 59, Almaty 050000, Kazakhstan. FAU - Leontyeva, Xeniya AU - Leontyeva X AUID- ORCID: 0000-0002-4740-1720 AD - D.V. Sokolsky Institute of Fuel, Catalysis and Electrochemistry JSC, 142 Kunayev Str., Almaty 050010, Kazakhstan. AD - Al-Farabi Kazakh National University, 71, Al-Farabi Avenue, Almaty 050012, Kazakhstan. FAU - Shaiakhmetov, Nurislam AU - Shaiakhmetov N AD - D.V. Sokolsky Institute of Fuel, Catalysis and Electrochemistry JSC, 142 Kunayev Str., Almaty 050010, Kazakhstan. AD - Kazakh British Technical University, Tole bi Street, 59, Almaty 050000, Kazakhstan. FAU - Boribaiuly, Batyr AU - Boribaiuly B AUID- ORCID: 0009-0001-3705-7794 AD - D.V. Sokolsky Institute of Fuel, Catalysis and Electrochemistry JSC, 142 Kunayev Str., Almaty 050010, Kazakhstan. AD - Kazakh British Technical University, Tole bi Street, 59, Almaty 050000, Kazakhstan. FAU - Ismailova, Guzal AU - Ismailova G AD - Al-Farabi Kazakh National University, 71, Al-Farabi Avenue, Almaty 050012, Kazakhstan. FAU - Nurislamov, Ruslan AU - Nurislamov R AUID- ORCID: 0009-0007-3654-0315 AD - D.V. Sokolsky Institute of Fuel, Catalysis and Electrochemistry JSC, 142 Kunayev Str., Almaty 050010, Kazakhstan. FAU - Khussurova, Gulinur AU - Khussurova G AUID- ORCID: 0000-0001-8700-7472 AD - D.V. Sokolsky Institute of Fuel, Catalysis and Electrochemistry JSC, 142 Kunayev Str., Almaty 050010, Kazakhstan. FAU - Kudryashov, Vladislav AU - Kudryashov V AUID- ORCID: 0000-0002-5667-611X AD - D.V. Sokolsky Institute of Fuel, Catalysis and Electrochemistry JSC, 142 Kunayev Str., Almaty 050010, Kazakhstan. AD - Al-Farabi Kazakh National University, 71, Al-Farabi Avenue, Almaty 050012, Kazakhstan. AD - Renewable Energy Lab, "National laboratory Astana", Nazarbayev University, 53 Kabanbay Batyr Ave., Astana 010000, Kazakhstan. LA - eng GR - BR24992812/Committee of Science of the Ministry of Science and Higher Education of the Republic of Kazakh-stan/ PT - Journal Article DEP - 20261018 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 SB - IM PMC - PMC13707759 OTO - NOTNLM OT - corrosion rate OT - direct and inverse emulsions OT - electrochemical protocol limitations OT - oil-brine electrolyte OT - organic corrosion inhibitors OT - steel corrosion COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:46 PHST- 2026/09/24 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - molecules31203743 [pii] AID - molecules-31-03743 [pii] AID - 10.3390/molecules31203743 [doi] PST - epublish SO - Molecules. 2026 Oct 18;31(20):3743. doi: 10.3390/molecules31203743. PMID- 42943811 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 18 TI - Reaction Studies on the Conversion of Aryl Methyl Halides to Aryl Carbonyl Compounds Using Ph(4)PBr/EDTA/CaF(2) as a Catalytic Oxidation System. LID - 10.3390/molecules31203742 [doi] LID - 3742 AB - We have developed an efficient protocol for the selective oxidation of arylmethyl halides to aromatic carbonyl compounds. This transformation employs tetraphenylphosphonium bromide (Ph(4)PBr) as both an oxidant and phase-transfer catalyst (PTC), in combination with EDTA and CaF(2) as cocatalysts. Under mild reaction conditions, the method enables highly selective conversion of C-X bonds (X = Cl, Br) to carbonyl (C = O) functionalities. A diverse range of aromatic carbonyl products-including aldehydes, ketones, alpha-diketones, alpha-keto esters, and anhydrides-were obtained in good to excellent yields (50~95%). FAU - Xu, Xianwei AU - Xu X AD - Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, College of Materials Science and Chemical Engineering, Harbin University of Science and Technology, Harbin 150080, China. FAU - Li, Sida AU - Li S AD - Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, College of Materials Science and Chemical Engineering, Harbin University of Science and Technology, Harbin 150080, China. FAU - Liu, Xincheng AU - Liu X AD - Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, College of Materials Science and Chemical Engineering, Harbin University of Science and Technology, Harbin 150080, China. FAU - You, Jun AU - You J AD - Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, College of Materials Science and Chemical Engineering, Harbin University of Science and Technology, Harbin 150080, China. FAU - Yu, Yanchao AU - Yu Y AD - Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, College of Materials Science and Chemical Engineering, Harbin University of Science and Technology, Harbin 150080, China. FAU - Liang, Yifang AU - Liang Y AD - Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, College of Materials Science and Chemical Engineering, Harbin University of Science and Technology, Harbin 150080, China. FAU - Wu, Wenju AU - Wu W AD - Key Laboratory of Green Chemical Engineering and Technology of College of Heilongjiang Province, College of Materials Science and Chemical Engineering, Harbin University of Science and Technology, Harbin 150080, China. LA - eng PT - Journal Article DEP - 20261018 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 SB - IM PMC - PMC13707991 OTO - NOTNLM OT - aromatic carbonyl compounds OT - arylmethyl halides OT - catalytic oxidation OT - tetraphenylphosphonium bromide COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:46 PHST- 2026/08/05 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - molecules31203742 [pii] AID - molecules-31-03742 [pii] AID - 10.3390/molecules31203742 [doi] PST - epublish SO - Molecules. 2026 Oct 18;31(20):3742. doi: 10.3390/molecules31203742. PMID- 42943806 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 18 TI - Modifications of 3D-Printed Adsorbent Materials for Pollutant Removal from Wastewaters. LID - 10.3390/molecules31203738 [doi] LID - 3738 AB - Adsorption remains one of the most practical and widely adopted strategies for removing pollutants from wastewater, yet conventional powder and granular adsorbents suffer from persistent limitations: poor recyclability, difficult solid-liquid separation, and limited control over selectivity in complex, multi-contaminant streams. Three-dimensional (3D) printing has emerged as a compelling alternative, offering precise control over geometry, porosity, and macrostructure that conventional shaping methods simply cannot achieve. However, the base polymers and inks most commonly used in additive manufacturing, such as PLA, ABS, PETG, and alginate, are chemically inert and lack inherent affinity for specific pollutants. Achieving meaningful adsorption capacity therefore depends critically on how and when the active functional component is introduced into the printed structure. This review examines the full spectrum of modification strategies applied to 3D-printed adsorbents for wastewater treatment, organized by the timing of modification relative to printing. Pre-printing approaches, including composite filament fabrication, functional ink formulation, and resin doping with active materials such as MOFs, biochar, zeolites, and metal oxides, are critically evaluated alongside post-printing strategies, including surface coating, nanoparticle decoration, chemical grafting, plasma treatment, and in situ growth of porous frameworks. For each approach, the implications for pollutant selectivity are assessed across the main contaminant classes: heavy metals, synthetic dyes, and organic micropollutants such as pharmaceuticals and Bisphenol A, which are considered according to their charge, hydrophobicity, and adsorption mechanism. Finally, key structure-modification relationships are identified, gaps in regeneration, competitive-adsorption, and real-wastewater testing are highlighted, and directions for rational, pollutant-targeted adsorbent design using additive manufacturing are outlined. FAU - Maroulas, Konstantinos N AU - Maroulas KN AUID- ORCID: 0009-0002-7597-7505 AD - Hephaestus Laboratory, School of Chemistry, Faculty of Sciences, Democritus University of Thrace, GR-65404 Kavala, Greece. FAU - Kyzas, George Z AU - Kyzas GZ AUID- ORCID: 0000-0003-1516-3761 AD - Hephaestus Laboratory, School of Chemistry, Faculty of Sciences, Democritus University of Thrace, GR-65404 Kavala, Greece. LA - eng PT - Journal Article PT - Review DEP - 20261018 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 SB - IM PMC - PMC13707822 OTO - NOTNLM OT - 3D-printing OT - additive manufacturing OT - adsorption OT - modifications OT - wastewater COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:46 PHST- 2026/09/05 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - molecules31203738 [pii] AID - molecules-31-03738 [pii] AID - 10.3390/molecules31203738 [doi] PST - epublish SO - Molecules. 2026 Oct 18;31(20):3738. doi: 10.3390/molecules31203738. PMID- 42943802 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 17 TI - Protecting-Group-Free and Minimally Protected Glycosylation: Recent Advances Toward Direct Glycosidic Bond Formation. LID - 10.3390/molecules31203733 [doi] LID - 3733 AB - Protecting-group-free glycosylation reduces the steps needed to convert native sugars into glycosides, but selectivity and product isolation remain major limits. This review examines methods published mainly from 2024 to August 2026. It compares donor preparation, activation conditions, linkage scope and stereochemical control. Glycosyl sulfonyl hydrazides can be prepared directly from reducing sugars and used in nickel-mediated arylation or copper-mediated C-, S- and Se-glycosylation. These donors can be isolated in large quantities by crystallization, but coupling often requires chromatography and high catalyst or reagent loadings. Ligand choice can give access to both anomers, although some methods require temporary hydroxyl protection. Photochemical thioglycoside activation, aqueous S-glycosylation and direct anomeric activation provide routes to other products. Selected examples are compared by reaction scale, isolated yield, purification and protection requirements. The comparison shows how simpler donor preparation can shorten a synthesis and why selectivity, reagent use and product isolation still affect method choice. FAU - Zhou, Zhenfei AU - Zhou Z AUID- ORCID: 0009-0005-5514-9712 AD - School of Pharmacy, Taizhou University, Taizhou 225300, China. FAU - Yang, Zhongmin AU - Yang Z AUID- ORCID: 0009-0001-4702-9826 AD - School of Pharmacy, Taizhou University, Taizhou 225300, China. LA - eng GR - TZXYNH2026QD019, TZXYNH2026QD020/Taizhou University/ PT - Journal Article PT - Review DEP - 20261017 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 SB - IM PMC - PMC13707743 OTO - NOTNLM OT - C-glycosides OT - glycosyl radicals OT - glycosylation OT - native sugars OT - nickel catalysis OT - protecting-group-free OT - redox-neutral cross-coupling OT - step economy OT - stereoretentive coupling OT - sulfonyl hydrazides COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:46 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - molecules31203733 [pii] AID - molecules-31-03733 [pii] AID - 10.3390/molecules31203733 [doi] PST - epublish SO - Molecules. 2026 Oct 17;31(20):3733. doi: 10.3390/molecules31203733. PMID- 42943798 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 17 TI - Coumarin-Ferulic Acid Hybrid Mediates HT-29-Selective Growth Inhibition and Proteomic Profiling. LID - 10.3390/molecules31203729 [doi] LID - 3729 AB - Coumarin-based molecular hybrids provide a framework for examining how structural changes influence cellular responses. This study compared four hybrids and three reference compounds across ten human cancer cell lines, with the lead hybrid also tested in non-cancerous Beas-2B cells, followed by functional assays and quantitative expression proteomics in HT-29 cells. Of 70 compound-cell-line combinations, the ferulic ester hybrid (FEC) was the only compound to yield a reproducibly measurable IC(50) within the primary screening range, reaching 42.8 +/- 4.7 microM in HT-29 cells. Replacing the ferulic moiety or the ester linkage reduced activity within this series. HCT116 cells did not reach 50% inhibition at up to 100 microM. Basal PPARgamma abundance did not consistently explain sensitivity across the panel. FEC reduced metabolic activity and colony formation, whereas annexin V/propidium iodide analysis showed a smaller apoptotic response. Proteomics identified coordinated decreases in DNA replication and translation proteins and a modest increase in endoplasmic reticulum protein-folding enzymes. These findings support a structure-dependent and cell-context-dependent antiproliferative response despite moderate potency. FAU - Krobthong, Sucheewin AU - Krobthong S AUID- ORCID: 0000-0003-4428-4140 AD - Centre of Biopharmaceutical Science for Healthy Ageing, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand. FAU - Yang, Meng Chieh AU - Yang MC AD - Centre of Biopharmaceutical Science for Healthy Ageing, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand. FAU - Rukthong, Pattarawit AU - Rukthong P AUID- ORCID: 0000-0002-7181-4226 AD - Department of Pharmaceutical Technology, Faculty of Pharmacy, Srinakharinwirot University, Ongkharak, Nakhonnayok 25120, Thailand. AD - Center for Excellence in Plant and Herbal Innovation Research, Strategic Wisdom and Research Institute, Srinakharinwirot University, Nakornnayok 26120, Thailand. FAU - Visansirikul, Satsawat AU - Visansirikul S AUID- ORCID: 0000-0002-2695-0974 AD - Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Si Ayutthaya Rd, Bangkok 10400, Thailand. AD - Unit of Compound Library for Drug Discovery, Mahidol University, Bangkok 10400, Thailand. FAU - Sripha, Kittisak AU - Sripha K AUID- ORCID: 0000-0003-4530-4684 AD - Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Si Ayutthaya Rd, Bangkok 10400, Thailand. AD - Unit of Compound Library for Drug Discovery, Mahidol University, Bangkok 10400, Thailand. FAU - Maiuthed, Arnatchai AU - Maiuthed A AUID- ORCID: 0000-0001-6528-7346 AD - Centre of Biopharmaceutical Science for Healthy Ageing, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand. AD - Department of Pharmacology, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand. LA - eng GR - FF-049/2566/Mahidol University/ GR - MRCMGR 01/2564 and A48/2563/Mahidol University/ GR - C10F640366/Program Management Unit of Competitiveness/ PT - Journal Article DEP - 20261017 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Coumarins) RN - 0 (Coumaric Acids) RN - AVM951ZWST (ferulic acid) RN - A4VZ22K1WT (coumarin) RN - 0 (Antineoplastic Agents) SB - IM MH - Humans MH - *Coumarins/chemistry/pharmacology MH - *Proteomics/methods MH - *Coumaric Acids/chemistry/pharmacology MH - Cell Proliferation/drug effects MH - HT29 Cells MH - *Antineoplastic Agents/pharmacology/chemistry MH - Cell Line, Tumor MH - Apoptosis/drug effects MH - HCT116 Cells PMC - PMC13707454 OTO - NOTNLM OT - HT-29 OT - colorectal carcinoma OT - coumarin hybrid OT - quantitative proteomics OT - structure activity relationship OT - unfolded protein response COIS- The authors declare no conflicts of interest. The funding bodies did not participate in the study's design, the collection of plant samples, data analysis, data interpretation, and the writing of the manuscript. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/17 CRDT- 2026/10/27 10:46 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - molecules31203729 [pii] AID - molecules-31-03729 [pii] AID - 10.3390/molecules31203729 [doi] PST - epublish SO - Molecules. 2026 Oct 17;31(20):3729. doi: 10.3390/molecules31203729. PMID- 42943795 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 17 TI - Selecting Administration Routes for Therapeutic Extracellular Vesicles to Achieve Optimal Drug Delivery. LID - 10.3390/molecules31203725 [doi] LID - 3725 AB - Extracellular vesicles (EVs) have emerged as promising therapeutic carriers owing to their biocompatibility, low immunogenicity, and ability to transport diverse biological cargo. However, successful EV-based therapy depends not only on carrier engineering but also on selecting an administration route appropriate for the target disease. This review examines major administration routes for therapeutic EVs, with lipid nanoparticles (LNPs) as a comparative platform, and discusses how anatomical barriers, biodistribution, clearance, inflammatory microenvironments, and target-cell localization influence delivery efficiency. Systemic administration provides broad accessibility but often results in substantial off-target distribution, whereas localized or barrier-bypassing routes can enhance exposure at specific pathological sites. Importantly, anatomical proximity does not necessarily guarantee efficient delivery, highlighting the need for quantitative assessment of target-tissue bioavailability. We propose a shift from a carrier-centered toward a disease- and route-centered paradigm integrating disease pathology, anatomical accessibility, biodistribution, and carrier engineering. FAU - Lee, Minah AU - Lee M AD - Department of Biopharmaceutical Engineering, College of Life Science and Nanotechnology, Hannam University, Daejeon 34054, Republic of Korea. FAU - Choi, Yeseul AU - Choi Y AD - Department of Biopharmaceutical Engineering, College of Life Science and Nanotechnology, Hannam University, Daejeon 34054, Republic of Korea. FAU - Yoon, Sung-Jin AU - Yoon SJ AUID- ORCID: 0000-0002-7372-0231 AD - Department of Functional Genomics, KRIBB School of Bioscience, University of Science and Technology (UST), Daejeon 34113, Republic of Korea. AD - Environmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea. FAU - Kim, Minseong AU - Kim M AUID- ORCID: 0000-0002-3927-4899 AD - Department of Biopharmaceutical Engineering, College of Life Science and Nanotechnology, Hannam University, Daejeon 34054, Republic of Korea. LA - eng GR - RS-2026-25483041/National Research Foundation of Korea/ GR - 2026-ANCHOR-06-013/Ministry of Education/ PT - Journal Article PT - Review DEP - 20261017 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Drug Carriers) RN - 0 (Lipids) SB - IM MH - *Extracellular Vesicles/chemistry/metabolism MH - Humans MH - *Drug Delivery Systems/methods MH - Animals MH - Tissue Distribution MH - Nanoparticles/chemistry MH - Drug Administration Routes MH - *Drug Carriers/chemistry MH - Lipids/chemistry PMC - PMC13707499 OTO - NOTNLM OT - administration routes OT - drug delivery OT - extracellular vesicles COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/17 CRDT- 2026/10/27 10:46 PHST- 2026/09/19 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - molecules31203725 [pii] AID - molecules-31-03725 [pii] AID - 10.3390/molecules31203725 [doi] PST - epublish SO - Molecules. 2026 Oct 17;31(20):3725. doi: 10.3390/molecules31203725. PMID- 42943794 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 17 TI - Determination of Selected Pesticide Residues in Wheat Flour by GC-MS. LID - 10.3390/molecules31203726 [doi] LID - 3726 AB - The widespread use of pesticides in modern agriculture contributes to a range of environmental and food-safety concerns, including the persistence of chemical residues in food products, contamination of soil and water, and potential risks to human and ecosystem health. Among food and feed commodities, cereal-based products represent an important potential source of dietary exposure due to their extensive consumption and the possibility of residue transfer from wheat grains to processed flours. In this study, a direct acetone extraction combined with gas chromatography-ion-trap mass spectrometry (GC-MS) was explored as a simplified analytical approach for the determination of selected pesticide residues in wheat flour. Nine pesticides were initially screened, and six compounds (ethoprophos, dimethoate, terbufos, diazinon, chlorpyrifos, and methoxychlor) were retained for method development based on their extractability and chromatographic behavior. Chromatographic and ionization conditions were optimized in Full Scan, SIM, and MS/MS modes, and matrix-matched calibration was applied to compensate for matrix-induced signal variations. The method was evaluated in a composite wheat-flour matrix, demonstrating acceptable process efficiency, a characterized matrix effect, and satisfactory quantitative performance for four analytes over the experimentally evaluated range of 5-50 microg/kg. Two commercial flour samples contained estimated diazinon concentrations of 0.4 and 1.0 microg/kg, respectively, which were further assessed using the standard addition method. Overall, this study provides a proof of concept for the use of direct acetone extraction coupled with ion-trap GC-MS for the exploratory monitoring of selected pesticide residues in wheat flour. Full method validation in accordance with SANTE/11312/2021 v2026 was outside the scope of this work. FAU - Aresta, Antonella Maria AU - Aresta AM AUID- ORCID: 0000-0002-1198-9104 AD - Department of Biosciences, Biotechnology and Environment, University of Bari "Aldo Moro", Via Edoardo Orabona 4, 70125 Bari, Italy. FAU - De Vietro, Nicoletta AU - De Vietro N AD - Department of Biosciences, Biotechnology and Environment, University of Bari "Aldo Moro", Via Edoardo Orabona 4, 70125 Bari, Italy. FAU - Zambonin, Carlo AU - Zambonin C AUID- ORCID: 0000-0002-0588-3688 AD - Department of Biosciences, Biotechnology and Environment, University of Bari "Aldo Moro", Via Edoardo Orabona 4, 70125 Bari, Italy. LA - eng PT - Journal Article DEP - 20261017 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Pesticide Residues) RN - YUS1M1Q929 (Diazinon) SB - IM MH - *Pesticide Residues/analysis MH - *Gas Chromatography-Mass Spectrometry/methods MH - *Flour/analysis MH - *Triticum/chemistry MH - *Food Contamination/analysis MH - Tandem Mass Spectrometry MH - Diazinon/analysis PMC - PMC13707824 OTO - NOTNLM OT - GC-MS OT - food safety OT - ion-trap mass spectrometry OT - organochlorine pesticides OT - organophosphorus pesticides OT - pesticide residues OT - wheat flour COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/17 CRDT- 2026/10/27 10:46 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - molecules31203726 [pii] AID - molecules-31-03726 [pii] AID - 10.3390/molecules31203726 [doi] PST - epublish SO - Molecules. 2026 Oct 17;31(20):3726. doi: 10.3390/molecules31203726. PMID- 42943792 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 16 TI - From Wild-Growing Plants to Promising Candidates for Hemp Variety Development: Morphological and Phytochemical Characterization of Macedonian Cannabis sativa L. LID - 10.3390/molecules31203723 [doi] LID - 3723 AB - BACKGROUND: Wild-growing Cannabis sativa L. represents an underexplored source of hemp-type plant material, but its value for future selection depends on standardized morphological and phytochemical characterization. This study evaluated cultivated Macedonian Cannabis sativa plants of wild-origin (CsW) in comparison with selected commercial cultivars and literature-reported European hemp varieties, based on the assessment of phenotypic differentiation and cannabinoid diversity. METHODS: Seeds from five wild-growing plant groups from two N. Macedonian regions, Kocani (W1, W2, W6) and Strumica (W3 and W4), were planted outdoors together with four commercial cultivars with differing cannabinoid composition. Morphological traits were assessed using selected UPOV descriptors and multivariate analysis. Cannabinoids were determined by HPLC-DAD. RESULTS: UPOV-based PCA clearly differentiated CsW from the commercial cultivars (R(2)X = 0.92, Q(2) = 0.86), while the expanded comparative UPOV analysis indicated phenotypic affinity with registered hemp-type material, particularly Kompolti, supporting its potential relevance as a phenotypic reference in future DUS-oriented evaluation. Among the cultivated wild-origin groups, W1 from the Kocani region showed the highest proportion of female plants (10:1), representing a favorable agronomic characteristic for flower-oriented production. All CsW were CBD-predominant chemotype III class, with total CBD of 5.14-7.61%, while total Delta9-THC did not exceed 0.20%. This CBD/THC balance positioned the CsW within the higher-CBD range reported for selected European hemp cultivars, while maintaining a favorable low-THC profile. CONCLUSIONS: CsW represent morphologically distinct, CBD-rich and low-THC plant material displaying characteristics consistent with a hemp-type profile. The combined UPOV and cannabinoid data support its relevance as a promising resource for further agronomic evaluation, phytochemical selection and future registration-oriented hemp research. FAU - Stoilkovska Gjorgievska, Veronika AU - Stoilkovska Gjorgievska V AUID- ORCID: 0000-0001-8656-1316 AD - Institute of Pharmacognosy, Faculty of Pharmacy, Ss. Cyril and Methodius University in Skopje, Majka Tereza 47, 1000 Skopje, North Macedonia. FAU - Cvetkovikj Karanfilova, Ivana AU - Cvetkovikj Karanfilova I AUID- ORCID: 0000-0002-4248-140X AD - Institute of Pharmacognosy, Faculty of Pharmacy, Ss. Cyril and Methodius University in Skopje, Majka Tereza 47, 1000 Skopje, North Macedonia. FAU - Geskovski, Nikola AU - Geskovski N AUID- ORCID: 0000-0002-2073-5632 AD - Institute of Pharmaceutical Technology, Faculty of Pharmacy, Ss. Cyril and Methodius University in Skopje, Majka Tereza 47, 1000 Skopje, North Macedonia. FAU - Trajkovska Matevska, Ana AU - Trajkovska Matevska A AUID- ORCID: 0000-0001-7541-5735 AD - Institute of Pharmacognosy, Faculty of Pharmacy, Ss. Cyril and Methodius University in Skopje, Majka Tereza 47, 1000 Skopje, North Macedonia. FAU - Davkova Stojanovski, Iskra AU - Davkova Stojanovski I AUID- ORCID: 0000-0001-7589-3817 AD - Institute of Pharmacognosy, Faculty of Pharmacy, Ss. Cyril and Methodius University in Skopje, Majka Tereza 47, 1000 Skopje, North Macedonia. FAU - Todorov, Filip AU - Todorov F AUID- ORCID: 0009-0006-0817-4883 AD - Institute of Pharmacognosy, Faculty of Pharmacy, Ss. Cyril and Methodius University in Skopje, Majka Tereza 47, 1000 Skopje, North Macedonia. FAU - Karapandzova, Marija AU - Karapandzova M AUID- ORCID: 0000-0002-4596-081X AD - Institute of Pharmacognosy, Faculty of Pharmacy, Ss. Cyril and Methodius University in Skopje, Majka Tereza 47, 1000 Skopje, North Macedonia. FAU - Stefkov, Gjoshe AU - Stefkov G AUID- ORCID: 0000-0002-5801-2931 AD - Institute of Pharmacognosy, Faculty of Pharmacy, Ss. Cyril and Methodius University in Skopje, Majka Tereza 47, 1000 Skopje, North Macedonia. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Phytochemicals) RN - 0 (Cannabinoids) SB - IM MH - *Cannabis/chemistry/growth & development/anatomy & histology MH - *Phytochemicals/chemistry/analysis MH - Cannabinoids/chemistry/analysis MH - Seeds/chemistry MH - Phenotype MH - Chromatography, High Pressure Liquid PMC - PMC13707986 OTO - NOTNLM OT - PCA OT - PLS-DA OT - UPOV descriptors OT - agronomic traits OT - cannabidiol (CBD) OT - chemotype OT - phytocannabinoids COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/16 CRDT- 2026/10/27 10:46 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - molecules31203723 [pii] AID - molecules-31-03723 [pii] AID - 10.3390/molecules31203723 [doi] PST - epublish SO - Molecules. 2026 Oct 16;31(20):3723. doi: 10.3390/molecules31203723. PMID- 42943790 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 16 TI - Waste-Derived Zeolite-P (GIS): Synthesis, Polymorphism, Characterization, and Adsorption Applications for Water Purification and Selective Gas Separation. LID - 10.3390/molecules31203720 [doi] LID - 3720 AB - Zeolite-P is a synthetic member of the gismondine (GIS) framework family and is intrinsically microporous, with intersecting eight-membered-ring channels. Mesoporosity reported for some Zeolite-P samples is therefore a secondary textural feature arising from interparticle voids, crystal aggregation, defects, or deliberately hierarchical architectures rather than from the GIS framework itself. This critical review focuses specifically on waste-derived GIS/Zeolite-P and integrates polymorph selection (Na-P1 and Na-P2), phase-purity assessment, precursor-to-property relationships, shaping, regeneration, and adsorption under increasingly realistic conditions. Structural and compositional evidence from X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), X-ray fluorescence (XRF), inductively coupled plasma optical emission spectroscopy (ICP-OES), solid-state (29)Si and (27)Al magic-angle-spinning nuclear magnetic resonance (MAS NMR), X-ray photoelectron spectroscopy (XPS), and Brunauer-Emmett-Teller (BET) analysis is evaluated in terms of what each technique can establish specifically for GIS materials. Waste-derived synthesis routes-including hydrothermal, alkali-fusion-assisted, microwave-assisted, ultrasound-assisted, sol-gel, and solvent-free methods-are compared without assuming that a waste precursor or a shorter synthesis is intrinsically lower-impact. Particular attention is paid to water purification, ion exchange, dehydration, and selective gas adsorption, while distinguishing single-solute batch tests from multicomponent, real-water, regeneration, and breakthrough evidence. The review also reconciles computational predictions with experiments: idealized pure-silica GIS models can overpredict CO(2) uptake relative to realistic aluminosilicate Na-P1/Na-P2, for which extra-framework cations, hydration, and restricted diffusion strongly affect accessibility. The resulting analysis identifies phase purity, matrix effects, regeneration, leaching safety, shaping, and process-level techno-economic/life-cycle metrics as the key barriers to translation of waste-derived Zeolite-P from laboratory studies to deployable adsorbents. FAU - Boudia, Rafik Abdelkrim AU - Boudia RA AUID- ORCID: 0009-0006-3338-8648 AD - Laboratory of Applied Organic Synthesis, Faculty of Exact and Applied Sciences, University Oran1 Ahmed Ben Bella, BP 1524 El M'Naouer, Oran 31000, Algeria. AD - Laboratory of Elaboration and Spectroscopic Characterizations of Structural and Electronic Properties Materials, Faculty of Technology, University of Saida Tahar Moulay, P.B. 138 Ennasr, Saida 20000, Algeria. FAU - Bellucci, Stefano AU - Bellucci S AUID- ORCID: 0000-0003-0326-6368 AD - Qi S.r.l., Via Monte d'Oro, 2/A, 00071 Pomezia, Italy. AD - Research Direction, Universidad ECOTEC, Km 13.5 Samborondon, Samborondon 092302, Ecuador. AD - National Institute of Materials Physics (NIMP), 405A Atomistilor Street, 077125 Magurele, Romania. FAU - Adjdir, Mehdi AU - Adjdir M AD - Laboratory of Applied Organic Synthesis, Faculty of Exact and Applied Sciences, University Oran1 Ahmed Ben Bella, BP 1524 El M'Naouer, Oran 31000, Algeria. AD - Laboratory of Elaboration and Spectroscopic Characterizations of Structural and Electronic Properties Materials, Faculty of Technology, University of Saida Tahar Moulay, P.B. 138 Ennasr, Saida 20000, Algeria. LA - eng GR - This work is supported in part by the Italian Ministry for University and Research (MUR) with the resources of the FISA - Fondo Italiano per le Scienze Applicate, for the year 2023, project FISA-2023-00229 "Advanced Composite nanomaterials for WAter Manag/Italian Ministry for University and Research (MUR) with the resources of the FISA ./ PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 SB - IM PMC - PMC13707876 OTO - NOTNLM OT - GIS polymorphs OT - hierarchical porosity OT - microporosity OT - phase purity OT - regeneration OT - selective gas separation OT - waste-derived zeolite-P OT - water purification COIS- Author Stefano Bellucci was employed by the company Qi S.r.l. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:46 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - molecules31203720 [pii] AID - molecules-31-03720 [pii] AID - 10.3390/molecules31203720 [doi] PST - epublish SO - Molecules. 2026 Oct 16;31(20):3720. doi: 10.3390/molecules31203720. PMID- 42943785 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 16 TI - Rethinking Solid-Phase Microextraction (SPME) for Bioanalysis: Workflow Optimisation in Automated Vacuum-Assisted Cooling SPME for Fatty Acid Monitoring in Faecal Waters. LID - 10.3390/molecules31203716 [doi] LID - 3716 AB - Short-chain fatty acids (SCFAs) are key biomarkers of gut microbiota and intestinal health, yet their analysis is challenging due to high volatility, hydrophilicity, and the complexity of the faecal matrix. This study introduces a novel, green, and fully automated workflow for monitoring SCFAs in faecal water samples. It combines microwave-assisted derivatisation, vacuum-assisted cooling multiple solid-phase microextraction (Vac-HS-cooling-MSPME), and fast gas chromatography-mass spectrometry (GC-MS). On-sample derivatisation with 2,3,4,5,6-pentafluorobenzyl bromide was optimised via design of experiments, reducing reaction time from 3 h to just 2 min at 100 degrees C while preserving high yields and selectivity. Integration of Vac-HS-cooling-MSPME with a fast GC column (5 m x 0.1 mm ID x 0.4 microm) increased sample throughput from two to approximately six samples per hour, with total chromatographic times of 5.24 min. MSPME specifically improved sensitivity for low-response analytes such as pentafluorobenzyl formate. The method demonstrated excellent linearity (R(2) > 0.998), low limits of detection (0.002-0.09 mg/L), good intra- and inter-day precision, accuracies exceeding 85%, and consistent recovery for each analyte (86-90%). The AGREE greenness score improved from 0.65 to 0.68, confirming alignment with green analytical chemistry principles. This system offers a high-throughput, sensitive, and eco-friendly tool for SCFA quantification, confirmed by the application on real paediatric faecal water samples. FAU - Putignani, Lorenza AU - Putignani L AUID- ORCID: 0000-0003-0134-2830 AD - Unit of Microbiomics and Research Unit of Microbiome, Bambino Gesu Children's Hospital, IRCCS, 00146 Rome, Italy. AD - Department of Life Sciences, Health and Health Professions, Link Campus University, 00165 Rome, Italy. FAU - Dugheri, Stefano AU - Dugheri S AUID- ORCID: 0000-0002-3078-7188 AD - Department of Life Sciences, Health and Health Professions, Link Campus University, 00165 Rome, Italy. FAU - Fanfani, Niccolo AU - Fanfani N AD - Department of Experimental and Clinical Medicine, University of Florence, 50121 Florence, Italy. FAU - Cappelli, Giovanni AU - Cappelli G AD - Division of Occupational Medicine, Careggi University Hospital, 50134 Florence, Italy. FAU - Rapi, Ilaria AU - Rapi I AUID- ORCID: 0009-0001-2412-5882 AD - Department of Experimental and Clinical Medicine, University of Florence, 50121 Florence, Italy. FAU - Santillo, Michele AU - Santillo M AUID- ORCID: 0009-0000-0876-4521 AD - Department of Experimental and Clinical Medicine, University of Florence, 50121 Florence, Italy. FAU - Marrubini, Giorgio AU - Marrubini G AUID- ORCID: 0000-0003-1399-5783 AD - Department of Drug Sciences, University of Pavia, 27100 Pavia, Italy. FAU - Marangelo, Chiara AU - Marangelo C AUID- ORCID: 0000-0002-6633-3938 AD - Research Unit of Microbiome, Bambino Gesu Children's Hospital, IRCCS, 00146 Rome, Italy. FAU - Vernocchi, Pamela AU - Vernocchi P AD - Research Unit of Microbiome, Bambino Gesu Children's Hospital, IRCCS, 00146 Rome, Italy. FAU - Del Chierico, Federica AU - Del Chierico F AUID- ORCID: 0000-0002-4204-4736 AD - Research Unit of Microbiome, Bambino Gesu Children's Hospital, IRCCS, 00146 Rome, Italy. FAU - Braghini, Maria Rita AU - Braghini MR AD - Research Unit of Genetics of Complex Phenotypes, Bambino Gesu Children's Hospital, IRCCS, 00146 Rome, Italy. FAU - Andolina, Giulia AU - Andolina G AD - Research Unit of Genetics of Complex Phenotypes, Bambino Gesu Children's Hospital, IRCCS, 00146 Rome, Italy. FAU - Alisi, Anna AU - Alisi A AUID- ORCID: 0000-0001-7241-6329 AD - Research Unit of Genetics of Complex Phenotypes, Bambino Gesu Children's Hospital, IRCCS, 00146 Rome, Italy. FAU - Baldassarre, Antonio AU - Baldassarre A AUID- ORCID: 0000-0002-6124-3570 AD - Department of Experimental and Clinical Medicine, University of Florence, 50121 Florence, Italy. AD - Division of Occupational Medicine, Careggi University Hospital, 50134 Florence, Italy. FAU - Mucci, Nicola AU - Mucci N AUID- ORCID: 0000-0003-0579-1035 AD - Department of Experimental and Clinical Medicine, University of Florence, 50121 Florence, Italy. AD - Division of Occupational Medicine, Careggi University Hospital, 50134 Florence, Italy. LA - eng GR - European Union/ GR - Ministry of Health/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Fatty Acids, Volatile) RN - 0 (Fatty Acids) RN - 059QF0KO0R (Water) SB - IM MH - *Solid Phase Microextraction/methods MH - Gas Chromatography-Mass Spectrometry MH - *Feces/chemistry MH - *Fatty Acids, Volatile/analysis MH - *Fatty Acids/analysis MH - Workflow MH - Microwaves MH - Vacuum MH - *Water/chemistry MH - Automation PMC - PMC13707552 OTO - NOTNLM OT - faecal waters OT - gas chromatography-mass spectrometry OT - green analytical chemistry OT - gut microbiota biomarkers OT - microwave-assisted derivatisation OT - short-chain fatty acids OT - solid-phase microextraction COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/16 CRDT- 2026/10/27 10:46 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - molecules31203716 [pii] AID - molecules-31-03716 [pii] AID - 10.3390/molecules31203716 [doi] PST - epublish SO - Molecules. 2026 Oct 16;31(20):3716. doi: 10.3390/molecules31203716. PMID- 42943773 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 15 TI - Pyridine-3-Carboxamide-1,3,4-Thiadiazole Hybrids as Antimicrobial Agents: Synthesis, Biological Evaluation, In Vivo Toxicity, and Computational Studies. LID - 10.3390/molecules31203700 [doi] LID - 3700 AB - The rapid emergence of antimicrobial resistance necessitates the development of novel antimicrobial agents with improved efficacy and favorable safety profiles. In this study, a new series of pyridine-3-carboxamide-1,3,4-thiadiazole hybrids and their corresponding N-Mannich bases bearing structurally diverse piperazine substituents were designed, synthesized, and characterized by FT-IR, (1)H/(13)C NMR, and HR-MS. Their antimicrobial activity was evaluated against reference and clinical bacterial and fungal strains using broth microdilution and agar diffusion assays. The compounds exhibited selective activity against Gram-positive bacteria, with minimum inhibitory concentrations of 0.034-0.069 mg/mL against Staphylococcus aureus ATCC 33591 and bactericidal effects reflected by MBC values comparable to MICs. No activity was observed against Pseudomonas aeruginosa or Candida albicans, and the compounds were ineffective in eradicating preformed staphylococcal biofilms. In vivo toxicity studies using the Galleria mellonella model indicated no-to-low apparent acute toxicity under the applied experimental conditions for most derivatives. DFT and QTAIM analyses revealed substituent-dependent electronic and conformational differences, while molecular docking identified DNA gyrase as the most likely molecular target, with compounds 6 and 7 showing the strongest predicted binding. These results identify pyridine-3-carboxamide-1,3,4-thiadiazole Mannich bases as promising leads for developing new antibacterial agents against Gram-positive pathogens. FAU - Swiatek, Piotr AU - Swiatek P AUID- ORCID: 0000-0003-4613-025X AD - Department of Medicinal Chemistry, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland. FAU - Strzelecka, Malgorzata AU - Strzelecka M AUID- ORCID: 0000-0002-5689-4889 AD - Department of Medicinal Chemistry, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland. FAU - Szczukowski, Lukasz AU - Szczukowski L AUID- ORCID: 0000-0002-9768-6269 AD - Department of Medicinal Chemistry, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland. FAU - Jezierska, Aneta AU - Jezierska A AUID- ORCID: 0000-0001-6601-9124 AD - Faculty of Chemistry, University of Wroclaw, F. Joliot-Curie 14, 50-383 Wroclaw, Poland. FAU - Panek, Jaroslaw J AU - Panek JJ AUID- ORCID: 0000-0002-2952-9739 AD - Faculty of Chemistry, University of Wroclaw, F. Joliot-Curie 14, 50-383 Wroclaw, Poland. FAU - Junka, Adam AU - Junka A AUID- ORCID: 0000-0002-7559-8903 AD - Platform for Unique Models Application, Department of Translational Technologies, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland. AD - College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China. FAU - Woyton, Aleksandra AU - Woyton A AUID- ORCID: 0000-0002-2542-928X AD - Platform for Unique Models Application, Department of Translational Technologies, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland. FAU - Lesyk, Roman AU - Lesyk R AUID- ORCID: 0000-0002-3322-0080 AD - Department of Pharmaceutical, Organic and Bioorganic Chemistry, Danylo Halytsky Lviv National Medical University, Pekarska 69, 79010 Lviv, Ukraine. AD - Molecular Design Center, Danylo Halytsky Lviv National Medical University, Pekarska 69, 79010 Lviv, Ukraine. AD - Department of Biotechnology and Cell Biology, Medical College, University of Information Technology and Management in Rzeszow, Sucharskiego 2, 35-225 Rzeszow, Poland. FAU - Glomb, Teresa AU - Glomb T AUID- ORCID: 0000-0003-0940-8136 AD - Department of Medicinal Chemistry, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland. LA - eng GR - IDUB.D070.24.026/Wroclaw Medical University/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Thiadiazoles) RN - 0 (Pyridines) RN - 0 (Anti-Infective Agents) RN - 14IAC3GH7G (1,3,4-thiadiazole) RN - 0 (Anti-Bacterial Agents) SB - IM MH - *Thiadiazoles/chemistry/pharmacology/chemical synthesis MH - Microbial Sensitivity Tests MH - Molecular Docking Simulation MH - *Pyridines/chemistry/pharmacology/chemical synthesis MH - Animals MH - *Anti-Infective Agents/pharmacology/chemical synthesis/chemistry MH - Candida albicans/drug effects MH - Staphylococcus aureus/drug effects MH - Molecular Structure MH - Structure-Activity Relationship MH - Pseudomonas aeruginosa/drug effects MH - Anti-Bacterial Agents/pharmacology/chemical synthesis/chemistry PMC - PMC13707785 OTO - NOTNLM OT - 1,3,4-thiadiazole OT - DFT OT - N-Mannich base OT - QTAIM OT - antimicrobial activity OT - molecular docking OT - pyridine-3-carboxamide COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/15 CRDT- 2026/10/27 10:46 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - molecules31203700 [pii] AID - molecules-31-03700 [pii] AID - 10.3390/molecules31203700 [doi] PST - epublish SO - Molecules. 2026 Oct 15;31(20):3700. doi: 10.3390/molecules31203700. PMID- 42943772 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 15 TI - Comparative Evaluation of Bioactive Compounds, Antioxidant Capacity, and Quality Traits of Raspberry Cultivars. LID - 10.3390/molecules31203703 [doi] LID - 3703 AB - Although raspberries are widely valued for their health-promoting properties, comprehensive comparative studies integrating pomological quality traits, phenolic compounds, and antioxidant capacities across cultivars with distinct fruit colors remain sparse. Sixteen raspberry cultivars, including 12 red-fruited, three yellow-fruited, and one cultivar with black-purple fruits, were evaluated at a single location during one growing season. This study evaluated the physicochemical parameters, bioactive compounds, antioxidant activity, and selected phenolic acids of red-, yellow-, and black-purple-fruited raspberry cultivars grown under the same environmental conditions. Antioxidant capacity was determined using FRAP and DPPH assays. The results revealed highly significant cultivar-dependent variations in dry matter, total soluble solids, titratable acidity, TSS/TA ratio, vitamin C, tannins, total phenolics, flavonoids, anthocyanins, and overall antioxidant performance. The black purplefruited cultivar stood out with exceptionally high levels of total phenolics, flavonoids, anthocyanins, and FRAP activity. Red cultivars exhibited wide phenotypic and biochemical variability, while yellow cultivars displayed minimal anthocyanin accumulation. Correlation analysis highlighted a strong relationship between antioxidant potential (FRAP) and flavonoid content, alongside moderate correlations with anthocyanins and vitamin C. Most targeted phenolic acids were either not detected or occurred at concentrations below the limit of quantification, with only a few quantifiable detections. These findings provide insight into cultivar-dependent variation in raspberry fruit quality and bioactive composition and may support the selection of cultivars for further evaluation according to specific quality traits. FAU - Buczynski, Kamil AU - Buczynski K AUID- ORCID: 0000-0003-2322-6881 AD - Institute of Horticulture Production, University of Life Sciences in Lublin, Gleboka 28, 20-612 Lublin, Poland. FAU - Kaplan, Magdalena AU - Kaplan M AUID- ORCID: 0000-0002-3833-9275 AD - Institute of Horticulture Production, University of Life Sciences in Lublin, Gleboka 28, 20-612 Lublin, Poland. FAU - Czernecki, Tomasz AU - Czernecki T AD - Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences in Lublin, Skromna 8, 20-704 Lublin, Poland. FAU - Stoj, Anna AU - Stoj A AUID- ORCID: 0000-0003-1824-2051 AD - Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences in Lublin, Skromna 8, 20-704 Lublin, Poland. LA - eng PT - Comparative Study PT - Journal Article DEP - 20261015 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Antioxidants) RN - 0 (Anthocyanins) RN - 0 (Phenols) RN - 0 (Flavonoids) RN - PQ6CK8PD0R (Ascorbic Acid) RN - 0 (Plant Extracts) RN - I3P9R8317T (phenolic acid) RN - 0 (Phytochemicals) RN - 0 (Hydroxybenzoates) SB - IM MH - *Rubus/chemistry MH - *Antioxidants/chemistry/pharmacology/analysis MH - *Fruit/chemistry MH - Anthocyanins/chemistry/analysis MH - Phenols/chemistry/analysis MH - Flavonoids/chemistry/analysis MH - Ascorbic Acid MH - Plant Extracts/chemistry MH - *Phytochemicals/chemistry/pharmacology MH - Hydroxybenzoates PMC - PMC13707578 OTO - NOTNLM OT - Rubus OT - anthocyanins OT - flavonoids OT - fruit quality OT - phenolic COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/15 CRDT- 2026/10/27 10:46 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - molecules31203703 [pii] AID - molecules-31-03703 [pii] AID - 10.3390/molecules31203703 [doi] PST - epublish SO - Molecules. 2026 Oct 15;31(20):3703. doi: 10.3390/molecules31203703. PMID- 42943763 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 15 TI - Study of Stabilization Mechanism of CO(2) Foam Flooding System Using Gas-Soluble Nonionic Surfactant and Nano-SiO(2). LID - 10.3390/molecules31203693 [doi] LID - 3693 AB - CO(2) flooding is essential for enhancing oil recovery in low-permeability reservoirs; however, the large viscosity contrast between CO(2) and crude oil leads to severe gas channeling and low sweep efficiency. In this study, a binary composite system was constructed with a gas-soluble nonionic surfactant (OXO1312) as the foaming core and nano-SiO(2) as the Pickering stabilizer. The solubility of the surfactants in scCO(2) was evaluated at 60 degrees C and 20 MPa using a variable-volume apparatus, the foam performance was assessed by the Waring Blender method, and the foam morphology was observed by optical microscopy. The nonionic surfactants exhibited markedly higher scCO(2) solubilities (>2 wt%) than the anionic surfactants (<1 wt%), following a clear carbon-chain-length window effect. OXO1312, combining high CO(2)-philicity with an appropriate EO number, was selected as the optimal foaming agent. The addition of 1.5 wt% nano-SiO(2) increased the drainage half-life of the OXO1312 system by 84% through pronounced Pickering stabilization. The binary system remained stable below 50 degrees C under moderate salinity, whereas the half-life declined sharply above 70 degrees C. These findings provide a theoretical basis for the formulation design of CO(2) foam flooding systems for CCUS-EOR. FAU - Yu, Jiuzheng AU - Yu J AD - Oil and Gas Technology Institute of PetroChina Changqing Oilfield Company, Xi'an 710018, China. AD - National Engineering Laboratory for Exploration and Development of Low Permeability Oil and Gas Fields, Xi'an 710018, China. FAU - Qu, Xiao AU - Qu X AD - Oil and Gas Technology Institute of PetroChina Changqing Oilfield Company, Xi'an 710018, China. AD - National Engineering Laboratory for Exploration and Development of Low Permeability Oil and Gas Fields, Xi'an 710018, China. FAU - Yu, Shichun AU - Yu S AD - Oil and Gas Technology Institute of PetroChina Changqing Oilfield Company, Xi'an 710018, China. AD - National Engineering Laboratory for Exploration and Development of Low Permeability Oil and Gas Fields, Xi'an 710018, China. FAU - Jia, Yuqin AU - Jia Y AD - Oil and Gas Technology Institute of PetroChina Changqing Oilfield Company, Xi'an 710018, China. AD - National Engineering Laboratory for Exploration and Development of Low Permeability Oil and Gas Fields, Xi'an 710018, China. FAU - Yi, Ping AU - Yi P AD - Oil and Gas Technology Institute of PetroChina Changqing Oilfield Company, Xi'an 710018, China. AD - National Engineering Laboratory for Exploration and Development of Low Permeability Oil and Gas Fields, Xi'an 710018, China. FAU - Tian, Minglei AU - Tian M AUID- ORCID: 0000-0003-4880-3139 AD - College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou 434023, China. LA - eng GR - 2025ZD1408303/Oil & Gas Major Project/ GR - 2023ZZ17YJ02/PetroChina Major Science and Technology Project/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 SB - IM PMC - PMC13707895 OTO - NOTNLM OT - CO2 foam flooding OT - drainage half-life OT - gas-soluble nonionic surfactant OT - nano-SiO2 OT - supercritical CO2 COIS- Jiuzheng Yu, Xiao Qu, Shichun Yu, Yuqin Jia and Ping Yi are employee of Oil and Gas Technology Institute of PetroChina Changqing Oilfield Company. The other authors declare no conflicts of interest. Oil and Gas Technology Institute of PetroChina Changqing Oilfield Company had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:46 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - molecules31203693 [pii] AID - molecules-31-03693 [pii] AID - 10.3390/molecules31203693 [doi] PST - epublish SO - Molecules. 2026 Oct 15;31(20):3693. doi: 10.3390/molecules31203693. PMID- 42943761 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 15 TI - New Organic Compounds Against Scedosporium and Mucorales: Antifungal Activity and Mechanisms of Cell Wall and Membrane Disruption. LID - 10.3390/molecules31203691 [doi] LID - 3691 AB - The emergence of infections caused by Scedosporium/Lomentospora and Mucorales species poses a major clinical challenge due to their intrinsic resistance to multiple antifungal drugs and the limited number of therapeutic options available. In this study, we evaluated the antifungal activity of synthetic organic compounds and characterized the mechanisms of antifungal activity. Eight compounds were initially screened against Scedosporium aurantiacum and Rhizopus oryzae. Compounds 2, 3, and 4 inhibited more than 60% of fungal growth and were selected for further characterization. Antifungal susceptibility assays revealed that compound 2 exhibited the strongest activity, with minimum inhibitory concentrations ranging from 12.5 to 25 mug/mL, whereas compounds 3 and 4 showed MIC values between 50 and 100 mug/mL. All compounds displayed fungistatic activity and significantly impaired fungal germination and biofilm development. Drug interaction analyses demonstrated additive or synergistic effects when the compounds were combined with clinically used antifungals. Investigations revealed that the compounds induced alterations in the fungal cell surface, including reductions in neutral lipids and chitin, increased membrane permeability, disruption of sterol-enriched membrane microdomains, and ultrastructural abnormalities. Exogenous ergosterol partially reversed the activity of compounds 2 and 3, suggesting interactions with membrane sterols. In silico toxicity prediction indicated generally favorable safety profiles. In summary, these findings identify synthetic organic compounds as promising scaffolds for antifungal drug discovery and highlight their potential to target fungal membrane and cell wall integrity in resistant filamentous pathogens. FAU - Rollin-Pinheiro, Rodrigo AU - Rollin-Pinheiro R AUID- ORCID: 0000-0002-8316-4670 AD - Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciencias Medicas, Universidade do Estado do Rio de Janeiro (UERJ), Rio de Janeiro 20550-170, Brazil. FAU - de Castro-Almeida, Yuri AU - de Castro-Almeida Y AUID- ORCID: 0009-0008-3081-3885 AD - Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-902, Brazil. FAU - Xisto, Mariana Ingrid Dutra da Silva AU - Xisto MIDDS AUID- ORCID: 0000-0002-1803-6381 AD - Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-902, Brazil. FAU - de Souza-Santos, Johnatha AU - de Souza-Santos J AD - Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-902, Brazil. FAU - de Moraes, Daniel Clemente AU - de Moraes DC AD - Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-902, Brazil. FAU - Vicente, Bruno AU - Vicente B AUID- ORCID: 0009-0004-6576-5829 AD - Laboratorio de Biologia Estrutural, Instituto Oswaldo Cruz, Fundacao Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-900, Brazil. FAU - Midlej, Victor AU - Midlej V AUID- ORCID: 0000-0001-9294-6597 AD - Laboratorio de Biologia Estrutural, Instituto Oswaldo Cruz, Fundacao Oswaldo Cruz (Fiocruz), Rio de Janeiro 21040-900, Brazil. FAU - Toledo, Fabiano Travanca AU - Toledo FT AD - Department of Fundamental Chemistry, Institute of Chemistry, University of Sao Paulo, Sao Paulo 05508-000, Brazil. FAU - Ferrarini, Renan AU - Ferrarini R AD - Department of Fundamental Chemistry, Institute of Chemistry, University of Sao Paulo, Sao Paulo 05508-000, Brazil. FAU - Ferreira, Glaucio Monteiro AU - Ferreira GM AUID- ORCID: 0000-0002-1952-9428 AD - Department of Fundamental Chemistry, Institute of Chemistry, University of Sao Paulo, Sao Paulo 05508-000, Brazil. FAU - Santos, Alcindo A Dos AU - Santos AAD AUID- ORCID: 0000-0003-0358-610X AD - Department of Fundamental Chemistry, Institute of Chemistry, University of Sao Paulo, Sao Paulo 05508-000, Brazil. FAU - Barreto-Bergter, Eliana AU - Barreto-Bergter E AUID- ORCID: 0000-0002-8944-9415 AD - Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-902, Brazil. FAU - Ferreira-Pereira, Antonio AU - Ferreira-Pereira A AUID- ORCID: 0000-0002-0474-8388 AD - Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Goes, Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-902, Brazil. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Antifungal Agents) RN - 0 (Organic Chemicals) SB - IM MH - *Antifungal Agents/pharmacology/chemistry MH - *Cell Wall/drug effects MH - *Scedosporium/drug effects MH - Microbial Sensitivity Tests MH - *Mucorales/drug effects MH - *Cell Membrane/drug effects MH - Biofilms/drug effects MH - *Organic Chemicals/pharmacology/chemistry PMC - PMC13707570 OTO - NOTNLM OT - Lomentospora prolificans OT - Mucorales OT - Scedosporium OT - antifungal agents OT - antifungal drug discovery OT - drug-resistant fungi OT - membrane permeability OT - organochalcogenides COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/15 CRDT- 2026/10/27 10:46 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - molecules31203691 [pii] AID - molecules-31-03691 [pii] AID - 10.3390/molecules31203691 [doi] PST - epublish SO - Molecules. 2026 Oct 15;31(20):3691. doi: 10.3390/molecules31203691. PMID- 42943759 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 15 TI - Laboratory-to-Industrial Translation in Plant-Derived Bioproduct Valorization: A Critical Integrative Review and Proposed Framework for Route-Level Evidence Assessment. LID - 10.3390/molecules31203689 [doi] LID - 3689 AB - Gross extraction yield alone cannot establish product conformity or broader economic and environmental performance. This critical integrative review examines evidence linking laboratory findings to industrially relevant claims for plant-derived bioproduct valorization. It synthesizes seven evidence streams and proposes specification-adjusted route accounting, connecting route identity, output states, and claim-specific requirements. Selected elements were illustrated retrospectively using 17 purposively selected evidence packages. Artemisinin retention decreased from 90.0% after maceration to 37.3% after crystallization on the same initial artemisinin basis. Higher grapefruit-pectin yield accompanied changes in product properties without establishing functional equivalence. Red-grape-pomace economic preference depended on price assumptions. For microencapsulated pomegranate-peel extract powder, the highest deterministic net present value and highest probability of a positive net present value occurred in different modeled scale scenarios. Its separately scoped life cycle assessment identified freeze-drying as a hotspot without establishing comparative environmental improvement. No selected package provided verifiable observed-displacement evidence at the documented access level. The framework distinguishes supported conclusions from unresolved requirements; the cases illustrate its logic rather than validate its performance. Purposive selection, limited source access, and single-author assessment constrain interpretation. Independent and prospective evaluation is needed before reproducibility or decision benefits can be established. FAU - Won, Hyo Jun AU - Won HJ AUID- ORCID: 0009-0006-5149-6060 AD - Independent Researcher, Seoul 06300, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 SB - IM PMC - PMC13707718 OTO - NOTNLM OT - agri-food byproducts OT - evidence assessment OT - functional equivalence OT - life cycle assessment OT - plant-derived bioproducts OT - product specifications OT - scale-up OT - techno-economic assessment COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:46 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - molecules31203689 [pii] AID - molecules-31-03689 [pii] AID - 10.3390/molecules31203689 [doi] PST - epublish SO - Molecules. 2026 Oct 15;31(20):3689. doi: 10.3390/molecules31203689. PMID- 42943756 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 14 TI - Comparative Conformational Sampling of Apo Adenylate Kinase by Enhanced Molecular Dynamics and a Sequence-Based Generative Ensemble Model. LID - 10.3390/molecules31203686 [doi] LID - 3686 AB - Conformational sampling remains a major challenge in molecular dynamics (MD) simulations of proteins with large-scale functional motions. Here, we use apo Escherichia coli adenylate kinase (AdK) as a benchmark for the open-closed transition and compare nine sampling protocols under a uniform explicit-solvent budget of 300 ns per method. The methods include unbiased MD, metadynamics, targeted MD (TMD), accelerated MD, simulated tempering, interpolation-based MD, and BioEmu. Closed-state sampling was evaluated relative to a reference-closed basin defined from ligand-stripped PDB 1AKE. TMD was the only method that both reached and maintained the closed reference, whereas unbiased MD did not enter this basin within 300 ns. Metadynamics increased sampling along the domain-distance coordinate but also promoted excessive LID opening, while simulated tempering showed a similar tendency. BioEmu generated closed-like conformations, but these states were not maintained during subsequent short MD simulations. Overall, the results show that collective-variable coverage does not necessarily correspond to stable target state occupancy and that different sampling methods provide different types of conformational information. These findings highlight the importance of selecting sampling strategies according to the specific scientific objective. FAU - Zhao, Qingling AU - Zhao Q AD - Library of Beijing Union University, Beijing 100101, China. AD - College of Biochemical Engineering, Beijing Union University, Beijing 100023, China. FAU - Cui, Ting AU - Cui T AD - Library of Beijing Union University, Beijing 100101, China. AD - College of Biochemical Engineering, Beijing Union University, Beijing 100023, China. FAU - Li, Ying AU - Li Y AUID- ORCID: 0000-0001-8321-022X AD - Library of Beijing Union University, Beijing 100101, China. AD - College of Biochemical Engineering, Beijing Union University, Beijing 100023, China. LA - eng GR - BPHR202203209/Beijing Municipal Government/ PT - Comparative Study PT - Journal Article DEP - 20261014 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - EC 2.7.4.3 (Adenylate Kinase) SB - IM MH - *Molecular Dynamics Simulation MH - *Adenylate Kinase/chemistry/metabolism MH - Protein Conformation MH - *Escherichia coli/enzymology PMC - PMC13707528 OTO - NOTNLM OT - BioEmu OT - Gaussian accelerated MD OT - adenylate kinase OT - conformational sampling OT - metadynamics OT - molecular dynamics OT - open-closed transition OT - simulated tempering OT - targeted MD COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/14 CRDT- 2026/10/27 10:46 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - molecules31203686 [pii] AID - molecules-31-03686 [pii] AID - 10.3390/molecules31203686 [doi] PST - epublish SO - Molecules. 2026 Oct 14;31(20):3686. doi: 10.3390/molecules31203686. PMID- 42943755 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 14 TI - Study of the Extraction Process, Chemical Composition, and Biological Activities of Total Flavonoids from Vine Tea Leaves. LID - 10.3390/molecules31203685 [doi] LID - 3685 AB - This study investigated ethanol-based extraction of total flavonoids from vine tea (Ampelopsis grossedentata) leaves and characterized their chemical composition and in vitro biological activities. Ultrasound-assisted and heat-reflux extraction conditions were first screened by single-factor experiments and then evaluated using unreplicated L9(3(3)) orthogonal designs. For heat reflux extraction, the solid-to-liquid ratio had the greatest effect on flavonoid yield among the factors evaluated. The highest observed yield (58.20 mg rutin equivalents [RE]/g) was obtained with 70% ethanol, a 1:10 solid-to-liquid ratio, and 60 min (TCY6), representing the best-performing condition within the L9 array. TCY3 and TCY9 were not additional optima; they were retained as high-yield heat reflux comparison extracts for subsequent biological evaluation. Because the L9 runs were not independently replicated, the selected condition should be regarded as provisional, pending confirmation with replicated extraction batches. UPLC-QTOF-MS/MS profiling generated 94 metabolite annotations, comprising 43 higher-confidence assignments confirmed by accurate-mass matching and comparison with authentic reference standards and 51 tentative assignments supported by high-resolution MS/MS fragmentation. Flavones, flavonols, and flavanones accounted for 25.53%, 18.09%, and 15.96% of the annotated features, respectively. The extracts exhibited strong antioxidant activity, as demonstrated by their DPPH and ABTS radical-scavenging activities and ferric-reducing antioxidant power. In addition, they inhibited the growth of Escherichia coli, Salmonella spp., and Staphylococcus aureus, with the strongest antibacterial activity observed against S. aureus. In lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages, TCY3, TCY6, and TCY9 significantly reduced malondialdehyde (MDA) levels while enhancing the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) (p < 0.01). Furthermore, all three extracts significantly increased the level of interleukin-10 (IL-10) while decreasing the production of the pro-inflammatory interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) (p < 0.01). Collectively, total flavonoids extracted from vine tea leaves under the optimized conditions exhibited potent antioxidant, antibacterial, and anti-inflammatory activities, highlighting their potential as natural bioactive ingredients for functional food and pharmaceutical applications. FAU - Zhang, Lihui AU - Zhang L AD - School of Pharmaceutical Sciences, Tongren Polytechnic University, No. 2 Ziyou Road, Bijiang District, Tongren 554300, China. FAU - Wu, Qiuya AU - Wu Q AD - School of Pharmaceutical Sciences, Tongren Polytechnic University, No. 2 Ziyou Road, Bijiang District, Tongren 554300, China. FAU - Yang, Xiao-Qun AU - Yang XQ AD - School of Pharmaceutical Sciences, Tongren Polytechnic University, No. 2 Ziyou Road, Bijiang District, Tongren 554300, China. FAU - Ren, Huijing AU - Ren H AUID- ORCID: 0009-0004-2711-9770 AD - School of Pharmaceutical Sciences, Tongren Polytechnic University, No. 2 Ziyou Road, Bijiang District, Tongren 554300, China. FAU - Yang, Lihong AU - Yang L AD - School of Pharmaceutical Sciences, Tongren Polytechnic University, No. 2 Ziyou Road, Bijiang District, Tongren 554300, China. FAU - Hu, Meizhong AU - Hu M AD - School of Pharmaceutical Sciences, Tongren Polytechnic University, No. 2 Ziyou Road, Bijiang District, Tongren 554300, China. FAU - Pan, Dongyu AU - Pan D AD - School of Pharmaceutical Sciences, Tongren Polytechnic University, No. 2 Ziyou Road, Bijiang District, Tongren 554300, China. FAU - Yang, Shuang AU - Yang S AD - School of Pharmaceutical Sciences, Tongren Polytechnic University, No. 2 Ziyou Road, Bijiang District, Tongren 554300, China. FAU - Su, Dingfeng AU - Su D AD - Guizhou Jiangkou Fanjingshan Medical Technology Group Co., Ltd., Tongren 554400, China. FAU - Yue, Wenrui AU - Yue W AD - Guizhou Jiangkou Fanjingshan Medical Technology Group Co., Ltd., Tongren 554400, China. FAU - Liu, Qin AU - Liu Q AD - Guizhou Jiangkou Fanjingshan Medical Technology Group Co., Ltd., Tongren 554400, China. FAU - Yan, Zhiqiang AU - Yan Z AD - Chongqing Academy of Animal Sciences, Chongqing 402460, China. FAU - Wen, Ting-Chi AU - Wen TC AD - Engineering Research Center of Southwest Bio-Pharmaceutical Resources, Ministry of Education, Guizhou University, Guiyang 550025, China. FAU - Phal, Pheaktra AU - Phal P AD - Engineering Research Center of Southwest Bio-Pharmaceutical Resources, Ministry of Education, Guizhou University, Guiyang 550025, China. LA - eng GR - (QKHPT-KXJZ [2025] 007)/Guizhou Provincial Science and Technology Department/ GR - (No. KXJZ [2025]002)/Guizhou Provincial Scientist Workstation Project China/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Flavonoids) RN - 0 (Antioxidants) RN - 0 (Plant Extracts) RN - 0 (Tea) RN - 0 (Anti-Bacterial Agents) SB - IM MH - *Flavonoids/pharmacology/chemistry/isolation & purification MH - *Plant Leaves/chemistry MH - Antioxidants/pharmacology/chemistry/isolation & purification MH - *Plant Extracts/chemistry/pharmacology MH - Mice MH - Animals MH - *Tea/chemistry MH - RAW 264.7 Cells MH - Anti-Bacterial Agents/pharmacology/chemistry MH - Staphylococcus aureus/drug effects MH - Tandem Mass Spectrometry MH - Microbial Sensitivity Tests PMC - PMC13707999 OTO - NOTNLM OT - biological activities OT - chemical composition OT - extraction process OT - total flavonoids OT - vine tea leaves COIS- Su Dingfeng, Wenrui Yue, and Qin Liu are employees of Guizhou Jiangkou Fanjingshan Medical Technology Group Co., Ltd. All other authors declare no conflicts of interest. Guizhou Jiangkou Fanjingshan Medical Technology Group Co., Ltd. had no role in the study design; data collection, analysis, or interpretation; preparation of the manuscript; or the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/14 CRDT- 2026/10/27 10:46 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - molecules31203685 [pii] AID - molecules-31-03685 [pii] AID - 10.3390/molecules31203685 [doi] PST - epublish SO - Molecules. 2026 Oct 14;31(20):3685. doi: 10.3390/molecules31203685. PMID- 42943752 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 14 TI - Synthesis of Arylpiperazine-Based CNS Ligands: Batch Disconnections and Their Continuous-Flow Implementation. LID - 10.3390/molecules31203682 [doi] LID - 3682 AB - The 1-arylpiperazine unit recurs throughout centrally acting drugs, particularly ligands of serotonin and dopamine receptors, where its modular architecture-aryl group, piperazine core, linker, terminal fragment-has made it a durable design template. Three principal intermodule disconnections-the aryl-nitrogen bond, the piperazine-linker bond, and the linker-terminal bond-provide an organizing framework for the routes reviewed here. This review compares representative batch routes with reported continuous-flow implementations, distinguishing related methodological precedents, individual transformations in drug-specific routes, and linked multistep sequences. Three observations emerge from the reviewed examples. First, the drug-directed flow routes examined use preformed arylpiperazine building blocks. Second, dechlorination reported during cariprazine reductive amination highlights the importance of catalyst and solvent selection for that substrate. Third, the reported flow implementations address selected process challenges, while the multistep examples highlight the importance of compatibility between reaction and work-up operations. These observations inform a practical framework for evaluating batch and flow options for individual routes. FAU - Jeong, Daeun AU - Jeong D AD - Department of Chemistry, Seoul Women's University, Seoul 01797, Republic of Korea. FAU - Han, Ji Myoung AU - Han JM AD - Department of Chemistry, Seoul Women's University, Seoul 01797, Republic of Korea. FAU - Lee, Ji Hoo AU - Lee JH AD - Department of Chemistry, Seoul Women's University, Seoul 01797, Republic of Korea. FAU - Oh, In Young AU - Oh IY AD - Department of Chemistry, Seoul Women's University, Seoul 01797, Republic of Korea. FAU - Cho, Ye Rim AU - Cho YR AD - Department of Chemistry, Seoul Women's University, Seoul 01797, Republic of Korea. FAU - Kwon, Yong-Ju AU - Kwon YJ AUID- ORCID: 0000-0001-9517-7885 AD - Department of Chemistry, Seoul Women's University, Seoul 01797, Republic of Korea. LA - eng GR - RS-2024-00360297/National Research Foundation of Korea/ GR - 2026-0276/Seoul Women's University/ PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 SB - IM PMC - PMC13707661 OTO - NOTNLM OT - CNS ligands OT - arylpiperazine OT - batch synthesis OT - chemoselective hydrogenation OT - continuous-flow chemistry OT - flow-oriented design OT - medicinal chemistry OT - process intensification OT - reductive amination OT - telescoped synthesis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:46 PHST- 2026/09/23 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - molecules31203682 [pii] AID - molecules-31-03682 [pii] AID - 10.3390/molecules31203682 [doi] PST - epublish SO - Molecules. 2026 Oct 14;31(20):3682. doi: 10.3390/molecules31203682. PMID- 42943746 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 14 TI - The Impact of rAdo and (OXO)dGuo on the Electronic Properties of Clustered DNA Lesions: A Theoretical Approach. LID - 10.3390/molecules31203673 [doi] LID - 3673 AB - Ribonucleotides and 7,8-dihydro-8-oxo-2'-deoxyguanosine ((O)G) are frequently incorporated into genomic DNA as non-canonical moieties; their local co-occurrence can lead to the formation of clustered DNA lesions (CDLs) which may not be susceptible to repair. Their estimated co-occurrence within two helical turns may reach ~650 incidents per diploid genome and can increase in pathological states. The results presented in this article show the influence of riboadenosine (rAdo) on the structural and electronic properties of double-stranded (ds) DNA containing (O)G. For this purpose, four ds-pentamers representing different mutual rAdo and (O)G topology, i.e., d[A(1)G(2)rA(3)(O)G(4)A(5)]*d[T(5)C(4)T(3)C(2)T(1)] (GrA(O)G-DNA), d[A(1)(O)G(2)rA(3)G(4)A(5)]*d[T(5)C(4)T(3)C(2)T(1)] ((O)GrAG-DNA), d[A(1)G(2)rA(3)G(4)A(5)]*d[T(5)C(4)(O)G(3)C(2)T(1)] (rA(O)G-DNA) and d[A(1)G(2)rA(3)(O)G(4)A(5)]*d[T(5)C(4)T(3)A(2)T(1)] ((O)GA-DNA), have been selected and theoretically investigated at a M06-2X/6-31++G**//M06-2X/D95**:M06-2X/sto-3G level of theory in aqueous solution. The study examines their spatial geometry, stacking interactions, charge and spin distributions, Fukui functions, global reactivity descriptors, and vertical/adiabatic ionisation potentials and electron affinities. The above parameters of ds-oligonucleotides were found to be strongly dependent on topology. rA(O)G-DNA was the most resistant to charge-induced structural rearrangement, whereas (O)GA-DNA was the most responsive and exhibited the highest electron-accepting tendency: its adiabatic electron affinity was 4.07 eV, compared to 2.01 eV for rA(O)G-DNA. Also, rA(O)G-DNA and (O)GA-DNA demonstrated proton transfer accompanying relaxation of the radical cation state together with charge-spin separation. These findings indicate that rAdo can reshape the electronic landscape of (O)G-containing CDLs and may influence DNA damage recognition and repair. Such topology-dependent effects may play important parts in genome instability and treatment-associated DNA damage responses. FAU - Karwowski, Boleslaw T AU - Karwowski BT AUID- ORCID: 0000-0001-6922-7834 AD - Nucleic Acids Damage Laboratory of Food Science Department, Faculty of Pharmacy, Medical University of Lodz, ul. Muszynskiego 1, 90-151 Lodz, Poland. LA - eng GR - 503/3-045-02/503-31-001/Medical University of Lodz/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 9007-49-2 (DNA) RN - 88847-89-6 (8-Hydroxy-2'-Deoxyguanosine) RN - G9481N71RO (Deoxyguanosine) SB - IM MH - *DNA Damage MH - *DNA/chemistry MH - 8-Hydroxy-2'-Deoxyguanosine/chemistry MH - Nucleic Acid Conformation MH - *Deoxyguanosine/analogs & derivatives/chemistry MH - Models, Molecular MH - Electrons PMC - PMC13707549 OTO - NOTNLM OT - 7,8-dihydro-8-oxo-2'-deoxyguanosine OT - Fukui functions OT - clustered DNA lesions OT - density functional theory OT - ribonucleosides OT - vertical and adiabatic ionisation potential and electron affinity COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/14 CRDT- 2026/10/27 10:46 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - molecules31203673 [pii] AID - molecules-31-03673 [pii] AID - 10.3390/molecules31203673 [doi] PST - epublish SO - Molecules. 2026 Oct 14;31(20):3673. doi: 10.3390/molecules31203673. PMID- 42943738 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 14 TI - Wax Deposition Inhibition Performance of a PAO/Pour Point Depressant/SiO(2)-IL Ternary System in High-Wax-Content Model Oil. LID - 10.3390/molecules31203667 [doi] LID - 3667 AB - Wax deposition in high-wax-content oils can impair low-temperature transport, while single flow improvers often provide limited control over the overall deposition process. To improve wax deposition inhibition through functional complementarity, a PAO/pour point depressant (PPD)/SiO(2)-IL ternary formulation was developed. Alkyl-imidazolium-functionalized SiO(2) materials with C2, C4, and C6 chains were prepared and characterized by FT-IR, TGA, and SEM. Wax deposition was evaluated using a static cold-finger method at a bulk-oil temperature of 55 degrees C and a cold-finger temperature of 25 degrees C. A model oil containing 20 wt% paraffin and a deposition time of 2 h was selected for formulation evaluation. The binary formulation containing 40 ppm PAO and 100 ppm JN-3 achieved a wax deposition inhibition efficiency of 67.53%. Among the SiO(2)-IL materials, Sil@HIL (C6) showed comparatively high performance, with its highest inhibition at 200 ppm within the tested dosage range. The resulting ternary formulation achieved 77.49% inhibition, which is 9.96 percentage points above the binary formulation. These findings indicate that incorporating Sil@HIL into the PAO/JN-3 formulation further improves wax deposition inhibition under the investigated static conditions. FAU - Zhang, Penggang AU - Zhang P AD - Tarim Oilfield Company, PetroChina, Korla 841000, China. FAU - Huang, Mingji AU - Huang M AD - Tarim Oilfield Company, PetroChina, Korla 841000, China. FAU - Liu, Jinsong AU - Liu J AD - Tarim Oilfield Company, PetroChina, Korla 841000, China. FAU - Deng, Banghui AU - Deng B AD - Tarim Oilfield Company, PetroChina, Korla 841000, China. FAU - Yang, Donglei AU - Yang D AD - Tarim Oilfield Company, PetroChina, Korla 841000, China. FAU - Zhu, Junkai AU - Zhu J AD - Tarim Oilfield Company, PetroChina, Korla 841000, China. FAU - Guo, Ying AU - Guo Y AD - Tarim Oilfield Company, PetroChina, Korla 841000, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 SB - IM PMC - PMC13707676 OTO - NOTNLM OT - PAO drag-reducing agent OT - SiO2-IL OT - pour point depressant OT - ternary formulation OT - wax deposition inhibition COIS- Penggang Zhang, Mingji Huang, Jinsong Liu, Banghui Deng, Donglei Yang, Junkai Zhu, and Ying Guo are employees of Tarim Oilfield Company, PetroChina. All authors declare no conflicts of interest. Tarim Oilfield Company, PetroChina had no role in the design of the study; in the collection, analysis, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:46 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - molecules31203667 [pii] AID - molecules-31-03667 [pii] AID - 10.3390/molecules31203667 [doi] PST - epublish SO - Molecules. 2026 Oct 14;31(20):3667. doi: 10.3390/molecules31203667. PMID- 42943737 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 13 TI - Structural Engineering Modulation of Metal Sulfide Photocatalysts from Charge Regulation Mechanisms to Practical Applications. LID - 10.3390/molecules31203666 [doi] LID - 3666 AB - Metal sulfide semiconductors have attracted considerable attention in solar-driven energy conversion and environmental remediation because of their strong visible-light response, tunable electronic structures, and abundant surface-active sites. Nevertheless, further improvements in their photocatalytic performance remain constrained by rapid charge-carrier recombination, photocorrosion, insufficient reaction selectivity, and limited operational stability under practical conditions. Existing reviews have largely focused on specific sulfide materials, individual photocatalytic reactions, or a single structural-regulation strategy, whereas the intrinsic relationships among material composition, structural engineering, charge-carrier dynamics, and application requirements have not been systematically examined. Following the conceptual framework of "material composition-structural regulation-charge-carrier dynamics-reaction pathways-engineering applications," this review provides a systematic overview of the design principles and application advances of metal sulfide photocatalysts. First, the crystal structures, band characteristics, surface-active sites, and photocorrosion behavior of different classes of metal sulfides are analyzed to clarify how their intrinsic properties govern catalytic processes. The effects of morphology engineering, defect regulation, compositional optimization, interface construction, and cocatalyst modification on light absorption, charge transport, and surface reaction kinetics are then discussed, with particular attention to how these strategies balance activity enhancement and stability retention. The distinctive features of metal sulfides in photocatalytic hydrogen evolution, biomass photoreforming, CO(2) reduction, H(2)O(2) synthesis, pollutant degradation, and photoelectrochemical sensing are further summarized, revealing the distinct requirements of different reaction systems for electronic structures and active sites. Finally, current challenges and future research directions are discussed in terms of mechanistic reliability, material stability, adaptability to realistic systems, and sustainable development. This review aims to establish an application-oriented framework for the rational design of metal sulfide photocatalysts and to provide a theoretical basis for developing efficient, stable, and sustainable photocatalytic systems. FAU - Bai, Guangteng AU - Bai G AD - Institute for Energy Research, Jiangsu University, Zhenjiang 212013, China. FAU - Luo, Xi AU - Luo X AD - Institute for Energy Research, Jiangsu University, Zhenjiang 212013, China. FAU - Mo, Zhao AU - Mo Z AUID- ORCID: 0000-0001-5824-3281 AD - Institute for Energy Research, Jiangsu University, Zhenjiang 212013, China. FAU - Liu, Xu AU - Liu X AD - Institute for Energy Research, Jiangsu University, Zhenjiang 212013, China. FAU - Jiang, Zaiyong AU - Jiang Z AD - School of Chemistry & Chemical Engineering and Environmental Engineering, Weifang University, Weifang 261061, China. FAU - Zhu, Xianglin AU - Zhu X AUID- ORCID: 0000-0002-2667-5298 AD - Institute for Energy Research, Jiangsu University, Zhenjiang 212013, China. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 SB - IM PMC - PMC13707852 OTO - NOTNLM OT - charge-carrier dynamics OT - energy and environmental applications OT - metal sulfides OT - photocatalysis OT - structural engineering COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:46 PHST- 2026/09/03 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - molecules31203666 [pii] AID - molecules-31-03666 [pii] AID - 10.3390/molecules31203666 [doi] PST - epublish SO - Molecules. 2026 Oct 13;31(20):3666. doi: 10.3390/molecules31203666. PMID- 42943734 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 13 TI - Polyphosphate-Functionalized PGMA-Grafted Mesoporous Silica Nanoparticles for Selective U(VI) Removal from Aqueous Solutions. LID - 10.3390/molecules31203664 [doi] LID - 3664 AB - Uranium(VI) contamination in aquatic environments poses significant environmental and public health concerns, creating a need for efficient and selective adsorbents. In this study, polyphosphate-functionalized poly(glycidyl methacrylate)-grafted mesoporous silica nanoparticles (PP-PGMA/MSNs) were successfully synthesized and evaluated for U(VI) removal from aqueous solutions. A key novelty of this work lies in the incorporation of phosphate-rich functionalities onto PGMA-grafted MSNs prepared via ATRP, together with the systematic investigation of their effects on U(VI) adsorption equilibrium and selectivity in multi-metal systems. Fourier-transform infrared spectroscopy (FTIR), Brunauer-Emmett-Teller (BET), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA) confirmed the successful incorporation of phosphate-containing functional groups onto the MSN surface. The adsorbent exhibited rapid adsorption, reaching equilibrium within 120 min, with optimum performance observed at pH 4-6. A maximum U(VI) removal efficiency of 73.81% was achieved at an initial concentration of 10 mg L(-1), while the maximum adsorption capacity reached 114.94 mg g(-1). Equilibrium data were well described by the Langmuir isotherm model (R(2) = 0.9697), indicating predominantly monolayer adsorption on relatively homogeneous adsorption sites. Despite a substantial reduction in surface area following functionalization, the material maintained a high adsorption capacity, suggesting that uranium uptake is governed primarily by interactions at phosphate-containing active sites. Furthermore, the adsorbent exhibited preferential affinity toward U(VI) in multi-metal systems containing Cr, Ni, Cu, Zn, Cd, and Pb ions, demonstrating enhanced selectivity toward uranyl species. The adsorption behavior suggests that phosphate functionalities serve as the primary binding sites through surface complexation and coordination interactions. These findings demonstrate the potential of PP-PGMA/MSNs as selective adsorbents for U(VI) removal from complex aqueous systems and environmental remediation applications. FAU - Alshammari, Abdulmalik S AU - Alshammari AS AUID- ORCID: 0009-0008-4407-9693 AD - MSc in Nuclear Engineering Program, College of Engineering, King Saud University, Riyadh 12372, Saudi Arabia. FAU - Alosime, Eid M AU - Alosime EM AUID- ORCID: 0000-0003-4925-7192 AD - King Abdulaziz City for Science and Technology (KACST), P.O. Box 6086, Riyadh 11442, Saudi Arabia. FAU - Basfar, Ahmed A AU - Basfar AA AUID- ORCID: 0009-0005-8593-8085 AD - MSc in Nuclear Engineering Program, College of Engineering, King Saud University, Riyadh 12372, Saudi Arabia. AD - Department of Mechanical Engineering, College of Engineering, King Saud University, Riyadh 12372, Saudi Arabia. LA - eng GR - ORF-2026-2135/King Saud University/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 SB - IM PMC - PMC13707873 OTO - NOTNLM OT - mesoporous-silica nanoparticles OT - polymer OT - surface-initiated atom transfer radical polymerization OT - uranium removal COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:46 PHST- 2026/09/21 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - molecules31203664 [pii] AID - molecules-31-03664 [pii] AID - 10.3390/molecules31203664 [doi] PST - epublish SO - Molecules. 2026 Oct 13;31(20):3664. doi: 10.3390/molecules31203664. PMID- 42943731 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 13 TI - Thermal Processing Shapes Phenolic Profiles in Iodine-Biofortified Kale: Cultivar- and Iodine-Treatment-Associated Patterns Revealed by LC-HRMS. LID - 10.3390/molecules31203660 [doi] LID - 3660 AB - The impact of culinary processing on the phenolic profile of iodine-biofortified kale remains insufficiently resolved at the compound level. Green- and red-leaved kale grown without iodine supplementation or biofortified with KIO(3) or 5,7-diiodo-8-quinolinol (DIQ) was subjected to blanching, boiling, or steaming. LC-HRMS profiling resulted in 135 phenolic annotations, including three compounds confirmed using authentic standards, while selected major phenolics were additionally quantified or semi-quantified by PDA detection. Multivariate analysis revealed pronounced cultivar-related differences. Among processed samples, thermal processing accounted for the largest proportion of phenolic variation (61.6% in green and 68.5% in red kale), while iodine treatment accounted for 9.3% and 7.2%, and the Processing x Iodine component for 24.2% and 19.6%, respectively. Compound-level analysis revealed pronounced differences between steamed and boiled samples, with predominantly higher relative LC-HRMS signals after steaming, especially in red-leaved kale. Processing-related phenolic patterns also differed among Control, KIO(3), and DIQ sample pools. Because each cultivar x iodine treatment combination represented a single pooled biological source, iodine-associated differences were interpreted descriptively rather than as inferential treatment effects. Overall, thermal processing was a major determinant of phenolic composition, while processing-related patterns varied among iodine-treatment pools. FAU - Kusznierewicz, Barbara AU - Kusznierewicz B AUID- ORCID: 0000-0001-8138-3872 AD - Department of Chemistry, Technology and Biotechnology of Food, Faculty of Chemistry, Gdansk University of Technology, 11/12 G. Narutowicza St., 80-233 Gdansk, Poland. FAU - Wasniowska, Justyna AU - Wasniowska J AD - Department of Human Nutrition and Dietetics, Faculty of Food Technology, University of Agriculture in Krakow, Al. Mickiewicza 21, 31-120 Krakow, Poland. FAU - Koronowicz, Aneta AU - Koronowicz A AUID- ORCID: 0000-0002-1653-2867 AD - Department of Human Nutrition and Dietetics, Faculty of Food Technology, University of Agriculture in Krakow, Al. Mickiewicza 21, 31-120 Krakow, Poland. LA - eng GR - UMO-2020/37/B/NZ9/02710/National Science Centre/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Phenols) RN - 9679TC07X4 (Iodine) SB - IM MH - *Phenols/analysis/chemistry MH - *Iodine/chemistry MH - *Brassica/chemistry MH - Liquid Chromatography-Mass Spectrometry MH - Chromatography, Liquid MH - Hot Temperature PMC - PMC13707524 OTO - NOTNLM OT - 5,7-diiodo-8-quinolinol OT - biofortification OT - curly kale OT - iodine OT - phenolic compounds OT - thermal processing COIS- Aneta Koronowicz reports financial support from the National Science Centre, Poland, and is an inventor on patent application P.443221 owned by the University of Agriculture in Krakow. The other authors declare no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/13 CRDT- 2026/10/27 10:46 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - molecules31203660 [pii] AID - molecules-31-03660 [pii] AID - 10.3390/molecules31203660 [doi] PST - epublish SO - Molecules. 2026 Oct 13;31(20):3660. doi: 10.3390/molecules31203660. PMID- 42943729 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 13 TI - Optimization of Supercritical CO(2) Extraction Conditions and Metabolite Composition of Chamaepericlymenum suecicum L. Berries from Two Locations in the Magadan Region. LID - 10.3390/molecules31203658 [doi] LID - 3658 AB - Supercritical fluid extraction (SFE) using CO(2) has many advantages for recovering bioactive compounds, such as high selectivity, minimal solvent use and preservation of thermolabile metabolites. This study optimized SFE for extracting Chamaepericlymenum suecicum (Eurasian dwarf cornel) berries and compared the metabolite profiles of samples from two locations in the Magadan region: Magadan and Ola. The extraction process was conducted at temperatures ranging from 100 to 400 degrees C and pressures ranging from 31 to 70 MPa, using 1% ethanol as a co-solvent. Global yield ranged from 2.60 to 8.45 mg/100 mg, with the highest yield (8.45 mg/100 mg) obtained at 250 bar and 55 degrees C. Two-way ANOVA revealed that temperature, pressure, and their interaction significantly influenced the extraction yield (p < 0.001). A total of 47 compounds were identified by HPLC-ion trap-MS/MS, including 33 polyphenolic compounds (flavones, flavonols, flavan-3-ols, anthocyanins, phenolic acids, coumarins, and lignans), as well as 14 other metabolites (including fatty acids, oxylipins, alkaloids, organic acids, sugars, naphthoquinones, and anabolic steroids). The metabolite profiles differed between the two locations: Magadan samples contained 26 unique polyphenolic compounds, while Ola samples were characterized by the presence of flavan-3-ols, which were absent in Magadan samples. Only nine compounds were shared between the two locations. The number of compounds ranged from 8 to 24, and the Shannon diversity index was higher in the Magadan samples (H' = 3.178 and 2.303) than in the Ola samples (H' = 2.079 and 2.398). These findings demonstrate that supercritical fluid extraction (SFE) combined with metabolomic analysis is a powerful tool for profiling C. suecicum berries, revealing significant geographic variation in their chemical composition and highlighting their potential as a source of bioactive compounds for functional foods and pharmaceutical applications. FAU - Razgonova, Mayya P AU - Razgonova MP AUID- ORCID: 0000-0002-9732-1649 AD - N.I. Vavilov All-Russian Institute of Plant Genetic Resources, B. Morskaya 42-44, Saint-Petersburg 190000, Russia. AD - Advanced Engineering School, Institute of Biotechnology, Bioengineering and Food Systems, Far Eastern Federal University, Fr. Russian, pos. Ajax, 10, Vladivostok 690922, Russia. FAU - Nawaz, Muhammad A AU - Nawaz MA AUID- ORCID: 0000-0001-9901-7555 AD - Higher Engineering School of Agrobiotechnology, National Research Tomsk State University, Lenin Ave, 36, Tomsk 634050, Russia. AD - Laboratory for Research and Application of Supercritical Fluid Technologies in Agro-Food Biotechnology, National Research Tomsk State University, Lenin Ave, 36, Tomsk 634050, Russia. FAU - Sabitov, Andrey S AU - Sabitov AS AUID- ORCID: 0009-0007-8798-6464 AD - N.I. Vavilov All-Russian Institute of Plant Genetic Resources, B. Morskaya 42-44, Saint-Petersburg 190000, Russia. FAU - Senotrusova, Tamara A AU - Senotrusova TA AD - Advanced Engineering School, Institute of Biotechnology, Bioengineering and Food Systems, Far Eastern Federal University, Fr. Russian, pos. Ajax, 10, Vladivostok 690922, Russia. FAU - Li, Natalya G AU - Li NG AD - Advanced Engineering School, Institute of Biotechnology, Bioengineering and Food Systems, Far Eastern Federal University, Fr. Russian, pos. Ajax, 10, Vladivostok 690922, Russia. FAU - Golokhvast, Kirill S AU - Golokhvast KS AUID- ORCID: 0000-0002-4873-2281 AD - Higher Engineering School of Agrobiotechnology, National Research Tomsk State University, Lenin Ave, 36, Tomsk 634050, Russia. AD - Siberian Federal Scientific Centre of Agrobiotechnology RAS, 7 Centralnaya Street, Krasnoobsk 633501, Russia. LA - eng GR - 23-74-00044/Ministry of Science and Higher Education/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 142M471B3J (Carbon Dioxide) RN - 0 (Plant Extracts) RN - 0 (Polyphenols) SB - IM MH - *Carbon Dioxide/chemistry MH - *Fruit/chemistry MH - *Chromatography, Supercritical Fluid/methods MH - *Plant Extracts/chemistry MH - Tandem Mass Spectrometry MH - Chromatography, High Pressure Liquid MH - Metabolomics/methods MH - Polyphenols/chemistry MH - Temperature PMC - PMC13707946 OTO - NOTNLM OT - Chamaepericlymenum suecicum L. OT - Cornus suecica L. OT - Eurasian dwarf cornel OT - SFE OT - metabotyping OT - supercritical CO2 extraction OT - tandem mass spectrometry COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/13 CRDT- 2026/10/27 10:46 PHST- 2026/07/02 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - molecules31203658 [pii] AID - molecules-31-03658 [pii] AID - 10.3390/molecules31203658 [doi] PST - epublish SO - Molecules. 2026 Oct 13;31(20):3658. doi: 10.3390/molecules31203658. PMID- 42943727 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 13 TI - Heme-Binding Groups in Cytochrome P450 Inhibitors: Coordination Mechanisms, Evidence Standards and Implications for CYP19A1 Ligand Design. LID - 10.3390/molecules31203656 [doi] LID - 3656 AB - Cytochrome P450 (CYP) enzymes are heme-thiolate monooxygenases whose inhibition can result from direct reversible axial ligation of the distal heme iron, perturbation of the axial-water environment, or metabolism-dependent heme interactions. This focused review critically examines the chemical classes used as putative heme-binding groups in small-molecule CYP inhibitors, with emphasis on imidazoles, 1,2,4-triazoles, pyridines, 1,2,3-triazoles, sulfur-containing heterocycles, hydroxamates, nitriles and isonitriles. We discuss the evidential limits of ligand-induced type II spectra, distinguish direct parent-ligand coordination from mechanism-based inactivation, and outline an evidence hierarchy integrating enzymology, UV-Vis spectroscopy, EPR-based methods, structural biology and QM/MM calculations. CYP19A1 is used as a medicinal-chemistry case study illustrating how donor identity, binding geometry, scaffold recognition and selectivity against drug-metabolising and steroidogenic CYP enzymes must be optimized concurrently. We propose practical design and validation principles for the development of selectively heme-directed CYP19A1 inhibitors. FAU - Kozyra, Pawel AU - Kozyra P AUID- ORCID: 0000-0001-8071-8503 AD - Department of Biomedical and Analytical Chemistry, The John Paul II Catholic University of Lublin, St. Konstantynow 1j, 20-708 Lublin, Poland. AD - AI Drug Discovery Center, The John Paul II Catholic University of Lublin, St. Konstantynow 1j, 20-708 Lublin, Poland. FAU - Maslyk, Maciej AU - Maslyk M AUID- ORCID: 0000-0003-0516-3231 AD - AI Drug Discovery Center, The John Paul II Catholic University of Lublin, St. Konstantynow 1j, 20-708 Lublin, Poland. AD - Department of Molecular Biology, The John Paul II Catholic University of Lublin, ul. Konstantynow 1i, 20-708 Lublin, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 42VZT0U6YR (Heme) RN - 0 (Ligands) RN - 0 (Cytochrome P-450 Enzyme Inhibitors) RN - EC 1.14.14.1 (Aromatase) RN - 9035-51-2 (Cytochrome P-450 Enzyme System) RN - EC 1.14.14.1 (CYP19A1 protein, human) RN - 0 (Imidazoles) SB - IM MH - *Heme/chemistry/metabolism MH - Ligands MH - *Cytochrome P-450 Enzyme Inhibitors/chemistry/pharmacology MH - Humans MH - *Aromatase/chemistry/metabolism MH - Cytochrome P-450 Enzyme System/chemistry/metabolism MH - Drug Design MH - Models, Molecular MH - Protein Binding MH - Imidazoles/chemistry PMC - PMC13707629 OTO - NOTNLM OT - CYP19A1 OT - aromatase OT - aromatase inhibitors OT - chelation OT - coordination mechanisms OT - cytochrome P450 OT - drug discovery OT - heme OT - heme-binding groups COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/13 CRDT- 2026/10/27 10:46 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - molecules31203656 [pii] AID - molecules-31-03656 [pii] AID - 10.3390/molecules31203656 [doi] PST - epublish SO - Molecules. 2026 Oct 13;31(20):3656. doi: 10.3390/molecules31203656. PMID- 42943726 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 13 TI - Elemental Changes in Riesling Wines Following Controlled Apple Juice Addition: An ICP-OES-Based Model Study. LID - 10.3390/molecules31203655 [doi] LID - 3655 AB - Wine adulteration with inexpensive fruit juices remains a significant authenticity issue. Apple juice, due to its neutral sensory properties, can be added to white wines without visual detection. While elemental analysis is widely applied in wine authentication, its suitability for detecting adulteration with low-mineral juices has not been systematically evaluated. In this study, 60 commercial white wines representing seven grape varieties and 45 Riesling samples intentionally adulterated with 5%, 10%, and 20% (v/v) apple juice were analyzed for 24 elements using ICP-OES. The data were evaluated using nonparametric statistical tests, including Mann-Whitney U, Wilcoxon signed-rank, and Friedman tests, with Holm correction for multiple comparisons, as well as Pearson correlation analysis and principal component analysis. Apple juice addition caused a decrease in most evaluated elements, especially Ca, Mg, Mn, P, S, and Sr, reflecting dilution of the wine matrix, whereas Na showed an opposite trend, particularly at the highest adulteration level. The reduced PCA model based on selected diagnostic variables provided clearer visualization of the adulteration-related pattern than the model based on all variables and highlighted the separation of samples adulterated at 20% (v/v). Apple juice addition also increased wine pH. The results demonstrate that ICP-OES data combined with statistical and chemometric analysis can reveal compositional changes associated with apple juice adulteration in a Riesling wine model. FAU - Gajek, Magdalena AU - Gajek M AD - Institute of General and Ecological Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland. FAU - Scibiorek, Joanna AU - Scibiorek J AD - Institute of General and Ecological Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland. FAU - Szynkowska-Jozwik, Malgorzata Iwona AU - Szynkowska-Jozwik MI AUID- ORCID: 0000-0003-1959-3849 AD - Institute of General and Ecological Chemistry, Faculty of Chemistry, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 SB - IM MH - *Wine/analysis MH - *Malus/chemistry MH - *Fruit and Vegetable Juices/analysis MH - Principal Component Analysis MH - *Food Contamination/analysis MH - Vitis/chemistry PMC - PMC13707753 OTO - NOTNLM OT - ICP-OES OT - Riesling wine OT - apple juice OT - chemometric analysis OT - multi-element analysis OT - nonparametric statistics OT - principal component analysis OT - wine adulteration COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/13 CRDT- 2026/10/27 10:46 PHST- 2026/08/17 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - molecules31203655 [pii] AID - molecules-31-03655 [pii] AID - 10.3390/molecules31203655 [doi] PST - epublish SO - Molecules. 2026 Oct 13;31(20):3655. doi: 10.3390/molecules31203655. PMID- 42943725 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 13 TI - Precursor-Concentration-Regulated CuO-CeO(2) Catalysts with Tailored Oxygen Species and Redox Behavior for Efficient C(3)H(6) Catalytic Combustion. LID - 10.3390/molecules31203653 [doi] LID - 3653 AB - Flame synthesis is a promising route for producing mixed-oxide catalysts, but the role of precursor concentration in regulating catalyst formation during self-sustained flame pyrolysis remains insufficiently understood. In this work, CuO-CeO(2) mixed-oxide catalysts were prepared by varying the total metallic precursor concentration from 0.04 to 0.28 mol.L(-1) while keeping the Cu/(Cu + Ce) molar ratio fixed at 35 mol.%. This strategy enables the influence of precursor concentration to be distinguished from that of metal composition and provides insight into the formation of CuO-CeO(2) catalysts under a self-sustained flame environment. The results show that precursor concentration strongly affects particle aggregation, crystallite growth, Cu species distribution and possible lattice incorporation, oxygen species distribution, and low-temperature reducibility. Among the prepared catalysts, CuCe0.20 exhibits the most favorable balance among textural characteristics, Cu-Ce interaction, oxygen species distribution and redox behavior, and surface reactive oxygen availability. As a result, CuCe0.20 achieves 90% C(3)H(6) conversion at 380 degrees C and nearly complete conversion at 450 degrees C. More importantly, it maintains stable activity during a 600 min test at 450 degrees C with an apparent CO(2) selectivity of 99.9%. Post-reaction characterization further reveals that the CeO(2) framework and Cu-Ce interaction are largely retained, while the relative contribution of lattice oxygen increases after long-term reaction, accompanied by retained low-temperature reducibility. This work extends the design of flame-made CuO-CeO(2) catalysts from composition optimization to precursor-concentration engineering and demonstrates a practical strategy for preparing stable non-noble metal catalysts for VOC catalytic combustion. FAU - Zhang, Shuo AU - Zhang S AD - Key Laboratory of Energy Thermal Conversion and Control, Ministry of Education, School of Energy and Environment, Southeast University, Nanjing 210096, China. FAU - Zhang, Tianze AU - Zhang T AD - Key Laboratory of Energy Thermal Conversion and Control, Ministry of Education, School of Energy and Environment, Southeast University, Nanjing 210096, China. FAU - Lu, Yuxiao AU - Lu Y AD - Key Laboratory of Energy Thermal Conversion and Control, Ministry of Education, School of Energy and Environment, Southeast University, Nanjing 210096, China. FAU - Meng, Shiya AU - Meng S AD - Key Laboratory of Energy Thermal Conversion and Control, Ministry of Education, School of Energy and Environment, Southeast University, Nanjing 210096, China. FAU - Sun, Zhenkun AU - Sun Z AD - Key Laboratory of Energy Thermal Conversion and Control, Ministry of Education, School of Energy and Environment, Southeast University, Nanjing 210096, China. FAU - Duan, Yuanqiang AU - Duan Y AD - Key Laboratory of Energy Thermal Conversion and Control, Ministry of Education, School of Energy and Environment, Southeast University, Nanjing 210096, China. LA - eng GR - No. 52506128; 52406127/National Natural Science Foundation of China/ GR - 2026M790626/China Postdoctoral Science Foundation/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 SB - IM PMC - PMC13707429 OTO - NOTNLM OT - C3H6 catalytic combustion OT - CuO-CeO2 mixed oxides OT - precursor-concentration engineering OT - redox behavior OT - self-sustained flame pyrolysis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:46 PHST- 2026/09/08 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - molecules31203653 [pii] AID - molecules-31-03653 [pii] AID - 10.3390/molecules31203653 [doi] PST - epublish SO - Molecules. 2026 Oct 13;31(20):3653. doi: 10.3390/molecules31203653. PMID- 42943718 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 13 TI - A Review on the Structural Evolution and Clinical Evidence of Boronic Acid beta-Lactamase Inhibitors. LID - 10.3390/molecules31203647 [doi] LID - 3647 AB - The spread of carbapenem-resistant Gram-negative bacilli has made beta-lactam/beta-lactamase inhibitor (BLI) combinations central to therapy. Boronic acids are the only BLI class whose chemistry can in principle reach all four Ambler classes, boron forms a tetrahedral adduct with the catalytic serine and can also coordinate the zinc ions of metallo-beta-lactamases. PubMed/MEDLINE and Web of Science were searched to 30 September 2026, together with ClinicalTrials.gov and the Protein Data Bank, for structural, biochemical, surveillance, and clinical data on vaborbactam, taniborbactam, and xeruborbactam. The three generations are compared across enzyme classes, the diazabicyclooctane durlobactam is used as a mechanistic comparator, and the pivotal phase 3 trials are appraised against the requirements of enzyme-guided therapy. The scaffold has advanced from KPC-selective monocyclic to bicyclic compounds that inhibit serine and subclass B1 metallo-beta-lactamases in vitro at nanomolar concentrations, but this breadth remains largely unrealized clinically. Reported potencies differ by one to two orders of magnitude between laboratories, coverage of IMP, SPM, and subclass B3 enzymes is incomplete, and no registrational trial has been designed around carbapenemase genotype. Partner beta-lactam selection and trial design now constrain the field more than target engagement does. FAU - Zou, Wenbo AU - Zou W AD - National Institutes for Drug Control, Beijing 102629, China. FAU - Zhu, Li AU - Zhu L AD - National Institutes for Drug Control, Beijing 102629, China. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (beta-Lactamase Inhibitors) RN - 0 (Boronic Acids) RN - EC 3.5.2.6 (beta-Lactamases) RN - 1C75676F8V (vaborbactam) RN - 0 (Anti-Bacterial Agents) SB - IM MH - *beta-Lactamase Inhibitors/chemistry/pharmacology/therapeutic use MH - *Boronic Acids/chemistry/pharmacology/therapeutic use MH - Humans MH - *beta-Lactamases/chemistry/metabolism MH - Anti-Bacterial Agents/chemistry/pharmacology PMC - PMC13707926 OTO - NOTNLM OT - boronic acid OT - carbapenemase OT - metallo-beta-lactamase OT - taniborbactam OT - vaborbactam OT - xeruborbactam COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/13 CRDT- 2026/10/27 10:46 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - molecules31203647 [pii] AID - molecules-31-03647 [pii] AID - 10.3390/molecules31203647 [doi] PST - epublish SO - Molecules. 2026 Oct 13;31(20):3647. doi: 10.3390/molecules31203647. PMID- 42943713 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 12 TI - Celastrol and Its Derivatives in Breast, Ovarian, and Cervical Cancer: Molecular Targets, Signalling Networks, and Nanocarrier-Based Delivery. LID - 10.3390/molecules31203641 [doi] LID - 3641 AB - Celastrol, a quinone methide pentacyclic triterpenoid obtained mainly from the roots of Tripterygium wilfordii Hook. f., forms covalent, often reversible, adducts with cysteine thiols in regulatory proteins, which underlies its multi-targeted action. This review summarises the preclinical evidence on celastrol and its derivatives in breast, ovarian, and cervical cancer and the nanocarriers designed to overcome its pharmacological limitations. Chemoproteomics has defined covalent targets at three distinguishable levels of evidence: residue-mapped and confirmed by mutagenesis or by genetic validation for IKKbeta, PRDX1, PKM2, HMGB1, and COMMD3; binding measured without mapping of the residue for the Hsp90-Cdc37 complex, the proteasome, further peroxiredoxins, and mitochondrial isocitrate dehydrogenases; and inferred from functional or degradation readouts for the remainder. Almost all of this work was performed outside the three tumour types, so the target catalogue applies to them by extrapolation. Across in vitro and in vivo models it induces cell-cycle arrest, apoptosis, and oxidative and mitochondrial stress, modulates NF-kappaB, PI3K/Akt/mTOR, JAK/STAT3, MAPK, Wnt/beta-catenin, and Hippo signalling, and limits migration, invasion, and angiogenesis. It also engages paraptosis, endoplasmic reticulum stress, pyroptosis, ferroptosis, cuproptosis, and immunogenic cell death, and remodels the tumour immune microenvironment. Modification at the C-20 carboxyl group and the A/B ring system, including proteolysis-targeting chimeras, has improved potency and selectivity. Because solubility, oral bioavailability, and the therapeutic window remain limiting, liposomal, micellar, carrier-free, and biomimetic nanocarriers have been developed to improve tumour selectivity and safety. Translation will depend on optimised derivatives, advanced delivery, sustainable production, rigorous pharmacokinetic and toxicological evaluation, and the pharmacoeconomic considerations examined here. FAU - Merecz-Sadowska, Anna AU - Merecz-Sadowska A AUID- ORCID: 0000-0001-9987-4573 AD - Department of Economic and Medical Informatics, University of Lodz, 90-214 Lodz, Poland. FAU - Kowalski, Maciej AU - Kowalski M AUID- ORCID: 0009-0000-8167-7010 AD - Students Research Group, Department of Medical Biology, Medical University of Lodz, 90-151 Lodz, Poland. FAU - Kowalczyk, Tomasz AU - Kowalczyk T AUID- ORCID: 0000-0002-4559-5015 AD - Department of Molecular Biotechnology and Genetics, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland. FAU - Trzcinski, Maksymilian AU - Trzcinski M AUID- ORCID: 0009-0006-7775-6940 AD - Students Research Club NANO, Department of Chemistry, Lodz University of Technology, Stefanowskiego 16, 90-537 Lodz, Poland. FAU - Muskala, Martyna AU - Muskala M AUID- ORCID: 0009-0001-8896-9320 AD - Students Research Group, Department of Medical Biology, Medical University of Lodz, 90-151 Lodz, Poland. FAU - Piekarski, Janusz AU - Piekarski J AD - Department of Surgical Oncology, Medical University of Lodz, 251 Pomorska St., 93-513 Lodz, Poland. FAU - Tylkowski, Bartosz AU - Tylkowski B AUID- ORCID: 0000-0002-4163-0178 AD - Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Jagiellonska 13-15, 85-067 Bydgoszcz, Poland. AD - Eurecat, Centre Tecnologic de Catalunya, C/Marcel.li Domingo 2, 43007 Tarragona, Spain. FAU - Picot, Laurent AU - Picot L AUID- ORCID: 0000-0003-4656-3557 AD - Littoral Environnement et Societes UMRi CNRS 7266 LIENSs, La Rochelle Universite, 17042 La Rochelle, France. FAU - Maggi, Filippo AU - Maggi F AUID- ORCID: 0000-0003-1375-4744 AD - Chemistry Interdisciplinary Project (ChIP) Research Center, School of Pharmacy, University of Camerino, Via Madonna delle Carceri, 62032 Camerino, Italy. FAU - Sitarek, Przemyslaw AU - Sitarek P AUID- ORCID: 0000-0001-5495-3550 AD - Department of Medical Biology, Medical University of Lodz, Muszynskiego 1, 90-151 Lodz, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - L8GG98663L (celastrol) RN - 0 (Pentacyclic Triterpenes) SB - IM MH - Humans MH - *Pentacyclic Triterpenes/chemistry/pharmacology MH - Female MH - Signal Transduction/drug effects MH - *Breast Neoplasms/drug therapy/metabolism MH - *Uterine Cervical Neoplasms/drug therapy/metabolism MH - *Ovarian Neoplasms/drug therapy/metabolism MH - Animals MH - Drug Delivery Systems PMC - PMC13707829 OTO - NOTNLM OT - Tripterygium wilfordii OT - breast cancer OT - celastrol OT - cervical cancer OT - nanomedicine OT - ovarian cancer OT - pharmacoeconomics OT - quinone methide OT - regulated cell death OT - tripterine COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/12 CRDT- 2026/10/27 10:46 PHST- 2026/09/12 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - molecules31203641 [pii] AID - molecules-31-03641 [pii] AID - 10.3390/molecules31203641 [doi] PST - epublish SO - Molecules. 2026 Oct 12;31(20):3641. doi: 10.3390/molecules31203641. PMID- 42943707 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 12 TI - Research on the Intervention of Bile Stasis-Induced Pruritus Using Small Molecules Targeting MRGPRX4 Through High-Throughput Screening. LID - 10.3390/molecules31203635 [doi] LID - 3635 AB - MRGPRX4, which is mainly expressed in human sensory neurons, is a key mediator of cholestatic pruritus, but clinically available small-molecule inhibitors targeting it remain very scarce; thus, this study aims to screen out novel active inhibitors targeting MRGPRX4. In this work, we performed high-throughput virtual screening of the small-molecule compound library with Schrodinger software targeting MRGPRX4, and selected the top five commercially available compounds with the highest scores from 13,144 candidate compounds. Molecular docking and molecular dynamics simulation were used for virtual validation, and the results confirmed that all five compounds could bind to MRGPRX4, among which 6'-O-malonyldaidzin showed the most favorable binding free energy and a compact binding pocket with the lowest SASA value. We further verified the effect of these compounds on Ca(2+) influx in 50 muM concentration in HEK293 cells overexpressing MRGPRX4, and confirmed the inhibitory activity of 6'-O-malonyldaidzin, whose binding interaction with MRGPRX4 was additionally verified by CETSA. In conclusion, this study identified 6'-O-malonyldaidzin as a promising lead compound for further pharmacological characterization and lead optimization, with potential for development as a targeted intervention for cholestatic pruritus. FAU - Liang, Qi AU - Liang Q AD - Chinese Medicine Germplasm Resources Innovation and Effective Uses Key Laboratory of Sichuan Province, School of Pharmacy, School of Modern Chinese Medicine Industry, Institute of Innovation, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. FAU - Kuang, Jiahao AU - Kuang J AUID- ORCID: 0009-0007-0937-7807 AD - Chinese Medicine Germplasm Resources Innovation and Effective Uses Key Laboratory of Sichuan Province, School of Pharmacy, School of Modern Chinese Medicine Industry, Institute of Innovation, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. FAU - Wang, Liangchen AU - Wang L AD - Chinese Medicine Germplasm Resources Innovation and Effective Uses Key Laboratory of Sichuan Province, School of Pharmacy, School of Modern Chinese Medicine Industry, Institute of Innovation, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. FAU - Gou, Hanghang AU - Gou H AD - Chinese Medicine Germplasm Resources Innovation and Effective Uses Key Laboratory of Sichuan Province, School of Pharmacy, School of Modern Chinese Medicine Industry, Institute of Innovation, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. FAU - Tang, Dandan AU - Tang D AD - School of Pharmacy, Sichuan College of Traditional Chinese Medicine, Mianyang 621000, China. FAU - Peng, Wei AU - Peng W AUID- ORCID: 0000-0003-0511-9564 AD - Chinese Medicine Germplasm Resources Innovation and Effective Uses Key Laboratory of Sichuan Province, School of Pharmacy, School of Modern Chinese Medicine Industry, Institute of Innovation, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. FAU - Wu, Chunjie AU - Wu C AD - Chinese Medicine Germplasm Resources Innovation and Effective Uses Key Laboratory of Sichuan Province, School of Pharmacy, School of Modern Chinese Medicine Industry, Institute of Innovation, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. LA - eng GR - 26MSZX296/Project on the Management Situation of Traditional Chinese Medicine in Sichuan Province/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Receptors, G-Protein-Coupled) RN - 0 (Small Molecule Libraries) RN - 0 (MRGPRX4 protein, human) RN - 0 (Receptors, Neuropeptide) RN - SY7Q814VUP (Calcium) SB - IM MH - Humans MH - *Receptors, G-Protein-Coupled/antagonists & inhibitors/metabolism/chemistry MH - *High-Throughput Screening Assays MH - HEK293 Cells MH - Molecular Docking Simulation MH - Molecular Dynamics Simulation MH - *Small Molecule Libraries/pharmacology/chemistry MH - *Pruritus/drug therapy/etiology/metabolism MH - Protein Binding MH - *Cholestasis/complications/drug therapy MH - *Receptors, Neuropeptide/antagonists & inhibitors/metabolism/chemistry MH - Calcium/metabolism PMC - PMC13707678 OTO - NOTNLM OT - MRGPRX4 OT - cholestasis-induced itching OT - small-molecule inhibitor OT - virtual screening COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/12 CRDT- 2026/10/27 10:46 PHST- 2026/09/06 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - molecules31203635 [pii] AID - molecules-31-03635 [pii] AID - 10.3390/molecules31203635 [doi] PST - epublish SO - Molecules. 2026 Oct 12;31(20):3635. doi: 10.3390/molecules31203635. PMID- 42943706 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 12 TI - Zingiberis Rhizoma Ethanol Extract Attenuates Recognition Memory Deficits and Neuroinflammation in a Mouse Model of Bilateral Common Carotid Artery Stenosis. LID - 10.3390/molecules31203634 [doi] LID - 3634 AB - Chronic cerebral hypoperfusion (CCH), a key contributor to vascular dementia (VaD), causes white matter injury and neuroinflammation. We evaluated the neuroprotective effects of 6-gingerol-rich Zingiberis Rhizoma ethanol extract (ZRex) in a mouse bilateral common carotid artery stenosis (BCAS) model. BCAS-operated C57BL/6 mice received oral ZRex (30, 100, or 300 mg/kg, three times weekly). Spatial working memory, recognition memory, glial activation, oligodendrocyte and myelin markers, RNA sequencing (RNA-seq), and 6-gingerol molecular docking were evaluated. ZRex partially restored recognition memory in the novel object recognition test without altering Y-maze spatial working memory. BCAS significantly increased platelet-derived growth factor receptor alpha-positive cell counts, which ZRex restored toward sham levels. ZRex also markedly attenuated cluster of differentiation 68 immunoreactivity. RNA-seq identified 479 BCAS-responsive candidate genes (fold-change-filtered) and demonstrated that ZRex selectively restored downregulated transcripts. Molecular docking revealed the strongest predicted binding of 6-gingerol to Mup19 (Vina score: -9.4 kcal/mol), followed by Serpina3, Saa2, Mup1, and F10. Overall, ZRex attenuated BCAS-induced recognition memory deficits and neuroinflammation by modulating metabolic and inflammatory pathways, potentially mediated by direct 6-gingerol interactions with candidate targets. These findings support ZRex as a promising botanical candidate for VaD. FAU - Huh, Taeyoung AU - Huh T AD - Department of Korean Medicine, College of Korean Medicine, Dong-Eui University, Busan 47227, Republic of Korea. FAU - Lim, Chiyeon AU - Lim C AD - Center for Advanced Medical Computing and Analysis, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA. FAU - Lim, Sehyun AU - Lim S AD - School of Public Health, Far East University, Eumseong 27601, Republic of Korea. FAU - Kim, Kyung-Min AU - Kim KM AD - Department of Korean Medicine, College of Korean Medicine, Dong-Eui University, Busan 47227, Republic of Korea. FAU - Cho, Suin AU - Cho S AUID- ORCID: 0000-0001-8507-1382 AD - Department of Korean Medicine, School of Korean Medicine, Pusan National University, Yangsan 50612, Republic of Korea. LA - eng PT - Journal Article DEP - 20261012 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Plant Extracts) RN - 925QK2Z900 (gingerol) RN - 0 (Catechols) RN - 0 (Neuroprotective Agents) RN - 3K9958V90M (Ethanol) RN - 0 (Fatty Alcohols) RN - C5529G5JPQ (ginger extract) SB - IM MH - Animals MH - *Plant Extracts/pharmacology/chemistry MH - Mice MH - *Memory Disorders/drug therapy/etiology MH - Disease Models, Animal MH - Molecular Docking Simulation MH - *Carotid Stenosis/drug therapy/complications MH - Male MH - *Neuroinflammatory Diseases/drug therapy/etiology MH - *Rhizome/chemistry MH - Mice, Inbred C57BL MH - Catechols/chemistry/pharmacology MH - Neuroprotective Agents/pharmacology/chemistry MH - Ethanol/chemistry MH - Fatty Alcohols/chemistry/pharmacology MH - *Zingiber officinale/chemistry PMC - PMC13707571 OTO - NOTNLM OT - Zingiberis Rhizoma OT - bilateral common carotid artery stenosis OT - neuroinflammation OT - vascular dementia COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/12 CRDT- 2026/10/27 10:46 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - molecules31203634 [pii] AID - molecules-31-03634 [pii] AID - 10.3390/molecules31203634 [doi] PST - epublish SO - Molecules. 2026 Oct 12;31(20):3634. doi: 10.3390/molecules31203634. PMID- 42943704 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 12 TI - LC-MS-Based Metabolomic Profiling of Excoecaria agallocha Leaf Extract: Integrated Antioxidant, Anti-Inflammatory, Cholinesterase Inhibitory and Neuroprotective Evaluation with AChE-Targeted Molecular Docking. LID - 10.3390/molecules31203632 [doi] LID - 3632 AB - Excoecaria agallocha L. is a medicinally important mangrove species containing diverse secondary metabolites with potential antioxidant, anti-inflammatory, and neuroprotective activities. This study investigated the multifunctional biological potential of E. agallocha leaves using petroleum ether, chloroform, ethyl acetate, methanol, and aqueous extracts. Preliminary phytochemical screening revealed a solvent-dependent distribution of constituents, while total phenolic and flavonoid contents were quantified in the methanolic and aqueous extracts. Antioxidant activity was evaluated using DPPH radical-scavenging and ferric-reducing antioxidant power (FRAP) assays, while anti-inflammatory activity was assessed using protein-denaturation and COX-2 inhibition assays, with aspirin and celecoxib as reference standards, respectively. Cytotoxicity of the methanolic and aqueous extracts was evaluated in L929 normal fibroblasts, and neuroprotective activity was assessed in SK-N-SH neuroblastoma cells exposed to trimethyltin (TMT), with the aqueous extract administered 1 h before TMT exposure. Apoptotic changes were evaluated using acridine orange/ethidium bromide (AO/EB) dual-fluorescence staining. Acetylcholinesterase (AChE) inhibition was determined using galantamine as the reference inhibitor. LC-MS was employed for putative metabolite profiling of the aqueous extract, followed by AChE-targeted molecular docking of selected identified compounds against human acetylcholinesterase (AChE; PDB ID 7E3D) and ProTox-II-based in silico toxicity prediction. The aqueous extract showed the highest total phenolic (717.81 microg/g) and flavonoid (485.16 microg/g) contents compared with the methanolic extract (562.43 and 334.00 microg/g, respectively) and demonstrated the strongest DPPH radical-scavenging activity among the extracts (IC(50) = 115.49 microg/mL) together with a marked ferric-reducing capacity. The aqueous extract also exhibited pronounced COX-2 inhibition (IC(50) = 39.77 microg/mL), whereas the methanolic extract showed greater inhibition of protein denaturation (IC(50) = 138.89 microg/mL). The aqueous extract displayed relatively low cytotoxicity toward L929 cells and markedly attenuated TMT-induced toxicity in SK-N-SH cells, with the greatest protection observed at the lowest pre-treatment concentration (20 microg/mL; 92.14 +/- 1.77% cell viability versus 16.92 +/- 1.55% with TMT alone). AChE inhibition by the aqueous extract increased concentration-dependently, with an IC(50) of 55.64 microg/mL. Consistent with these findings, AO/EB staining demonstrated an increased proportion of viable cells and reduced apoptotic cell populations following aqueous-extract pre-treatment. LC-MS profiling revealed a chemically diverse array of putatively identified metabolites. AChE-targeted molecular docking putatively identified 6-hydroxy-2-(4-methoxyphenyl)-4H-chromen-4-one and daidzein showed comparatively favorable docking profiles together with Class 5 predicted toxicity. Collectively, the present findings demonstrate that E. agallocha leaf extracts possess measurable antioxidant, anti-inflammatory, cholinesterase-inhibitory, and neuroprotective-related activities under the experimental conditions investigated, with the aqueous extract showing comparatively pronounced activity. These findings provide preliminary experimental and computational evidence supporting further bioassay-guided isolation, experimental validation of individual compounds, mechanistic studies, and in vivo evaluation to determine the neuroprotective potential and biological relevance of E. agallocha. FAU - Alshamrani, Ayed A AU - Alshamrani AA AUID- ORCID: 0000-0003-2384-943X AD - Department of Pharmacology and Toxicology, College of Pharmacy, Jazan University, Jazan 45142, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261012 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Cholinesterase Inhibitors) RN - 0 (Plant Extracts) RN - 0 (Antioxidants) RN - 0 (Neuroprotective Agents) RN - 0 (Anti-Inflammatory Agents) RN - EC 3.1.1.7 (Acetylcholinesterase) RN - 0 (Flavonoids) SB - IM MH - *Cholinesterase Inhibitors/pharmacology/chemistry MH - *Plant Extracts/chemistry/pharmacology MH - Molecular Docking Simulation MH - *Antioxidants/pharmacology/chemistry MH - *Plant Leaves/chemistry MH - *Neuroprotective Agents/pharmacology/chemistry MH - Humans MH - *Anti-Inflammatory Agents/pharmacology/chemistry MH - *Metabolomics/methods MH - Animals MH - *Euphorbiaceae/chemistry MH - *Acetylcholinesterase/metabolism/chemistry MH - Chromatography, Liquid MH - Mice MH - Cell Line, Tumor MH - Mass Spectrometry MH - Flavonoids/chemistry PMC - PMC13707391 OTO - NOTNLM OT - COX-2 inhibition OT - Excoecaria agallocha OT - acetylcholinesterase OT - antioxidant activity OT - neuroprotection OT - trimethyltin COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/12 CRDT- 2026/10/27 10:46 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - molecules31203632 [pii] AID - molecules-31-03632 [pii] AID - 10.3390/molecules31203632 [doi] PST - epublish SO - Molecules. 2026 Oct 12;31(20):3632. doi: 10.3390/molecules31203632. PMID- 42943700 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 12 TI - Tailoring Garlic Bioactive Profiles Through Green Extraction: Comparative Optimization of Microwave- and Ultrasound-Assisted Technologies. LID - 10.3390/molecules31203629 [doi] LID - 3629 AB - Garlic (Allium sativum L.) contains a wide range of bioactive compounds, including phenolic compounds, flavonoids, and organosulfur compounds. Efficient recovery of phenolic and organosulfur compounds from garlic requires extraction technologies capable of selectively preserving chemically distinct bioactive constituents. This study comparatively optimized microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE) to elucidate their selectivity toward different classes of garlic bioactive compounds using response surface methodology (RSM) based on a central composite design. The effects of various extraction parameters on total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity (DPPH, FRAP, and ABTS), and allicin content were investigated. Model performance varied by response: several equations supported prediction within the studied ranges, whereas the MAE TFC and UAE DPPH and ABTS models showed limited predictive ability and significant lack of fit despite the high predicted R(2). At the selected conditions, the measured MAE extract contained 58.29 mg allicin/100 g DW after 7 min, whereas the measured UAE extract had TPC and TFC values of 358.7 g GAE/100 g DW and 117.8 g CE/100 g DW, respectively. These observations indicate that the selected conditions produced different bioactive profiles, but do not establish a statistically robust optimum for every response included in the desirability calculation. FAU - Krstic, Marko AU - Krstic M AUID- ORCID: 0000-0003-1947-4794 AD - Faculty of Chemistry, University of Belgrade, Studenski trg 16, 11158 Belgrade, Serbia. FAU - Teslic, Nemanja AU - Teslic N AUID- ORCID: 0000-0002-8219-1909 AD - Institute of Food Technology, University of Novi Sad, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia. FAU - Andric, Filip AU - Andric F AUID- ORCID: 0000-0001-7932-833X AD - Faculty of Chemistry, University of Belgrade, Studenski trg 16, 11158 Belgrade, Serbia. FAU - Boskovic, Perica AU - Boskovic P AUID- ORCID: 0000-0002-0532-4739 AD - Department of Chemistry, Faculty of Science, University of Split, Rudera Boskovica 33, 21000 Split, Croatia. FAU - Pavlic, Branimir AU - Pavlic B AUID- ORCID: 0000-0002-3551-7478 AD - Faculty of Technology, University of Novi Sad, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia. LA - eng PT - Comparative Study PT - Journal Article DEP - 20261012 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Plant Extracts) RN - 0 (Antioxidants) RN - 0 (Phenols) RN - 0 (Sulfinic Acids) RN - 3C39BY17Y6 (allicin) RN - 0 (Disulfides) RN - 0 (Flavonoids) RN - 0 (Phytochemicals) SB - IM MH - *Garlic/chemistry MH - *Microwaves MH - *Plant Extracts/chemistry MH - Antioxidants/chemistry/isolation & purification/pharmacology MH - Phenols/chemistry MH - Sulfinic Acids/chemistry MH - Disulfides MH - Flavonoids/chemistry MH - *Green Chemistry Technology/methods MH - Ultrasonic Waves MH - *Phytochemicals/chemistry MH - Ultrasonics PMC - PMC13707732 OTO - NOTNLM OT - Allium species OT - allicin OT - antioxidant capacity OT - extraction selectivity OT - functional food ingredients OT - organosulfur compounds OT - phenolic compounds OT - phytochemicals COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/12 CRDT- 2026/10/27 10:46 PHST- 2026/09/05 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - molecules31203629 [pii] AID - molecules-31-03629 [pii] AID - 10.3390/molecules31203629 [doi] PST - epublish SO - Molecules. 2026 Oct 12;31(20):3629. doi: 10.3390/molecules31203629. PMID- 42943696 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 11 TI - Hemicyanine-Based Wash-Free Near-Infrared Fluorescent Probe for Imaging Amyloid-beta Aggregates in Alzheimer's Disease. LID - 10.3390/molecules31203624 [doi] LID - 3624 AB - As a key biomarker of Alzheimer's disease (AD), amyloid-beta (Abeta) is frequently employed for the diagnosis of AD. Near-infrared fluorescence (NIRF) imaging is a non-invasive, low-cost route for visualizing Abeta in vivo. However, most existing Abeta NIRF probes require tedious washing steps, show modest fluorescence turn-on (<50-fold), and stain normal tissue non-specifically. In this work, by fine-tuning the amphiphilicity of a hemicyanine scaffold to place its log P within the appropriate range, we identified P3 as a wash-free NIRF probe for Abeta aggregates. P3 bound Abeta aggregates with high affinity. In vivo, P3 distinguished AD mice from wild-type mice with a 4.8-fold signal ratio, together with adequate brain uptake (1.5 +/- 0.1 ID/g at 20 min) and rapid clearance. Unlike Thioflavin T, which indiscriminately labels Abeta aggregates and normal neurons, P3 specifically labeled Abeta aggregates with negligible background, which enabled wash-free imaging with a signal-to-noise ratio of 80 +/- 4. This work provides a wash-free, high-affinity NIRF probe for Abeta imaging. FAU - Zhao, Xueqi AU - Zhao X AD - Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China. FAU - Liang, Rundong AU - Liang R AD - Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China. FAU - Li, Yao AU - Li Y AUID- ORCID: 0009-0002-8906-8230 AD - Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China. FAU - Yan, Jiawei AU - Yan J AD - Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China. FAU - Zhang, Jing AU - Zhang J AD - Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China. FAU - Su, Zheng AU - Su Z AD - Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China. FAU - Liu, Yang AU - Liu Y AUID- ORCID: 0000-0002-9284-1944 AD - Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China. FAU - Yang, Huali AU - Yang H AD - Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China. FAU - Cheng, Maosheng AU - Cheng M AD - Key Laboratory of Structure-Based Drug Design & Discovery of Ministry of Education, School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China. LA - eng GR - 2024-MSLH-442/Natural Science Foundation Project of the Joint Program of Science and Technology Planning of Liaoning Province/ GR - YQ202306/"OutstandingYouth" Support Plan of Shenyang Pharmaceutical University/ GR - NO. LJ212510163020, China/the Scientific Research Funding Project of Education Department of Liaoning Province/ PT - Journal Article DEP - 20261011 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Amyloid beta-Peptides) RN - 0 (Fluorescent Dyes) RN - 0 (Carbocyanines) RN - 0 (Protein Aggregates) RN - 0 (tetramethylene hemicyanine) RN - 0 (Fluorescent Chemosensor Compounds) SB - IM MH - *Alzheimer Disease/metabolism/diagnostic imaging MH - Animals MH - *Amyloid beta-Peptides/metabolism/chemistry MH - *Fluorescent Dyes/chemistry MH - Mice MH - Humans MH - *Carbocyanines/chemistry MH - *Protein Aggregates MH - Fluorescent Chemosensor Compounds MH - Brain/metabolism MH - Optical Imaging MH - Disease Models, Animal PMC - PMC13707792 OTO - NOTNLM OT - Alzheimer's disease OT - Abeta aggregates OT - NIRF Probe OT - fluorescence imaging OT - log P OT - wash-free COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/11 CRDT- 2026/10/27 10:46 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - molecules31203624 [pii] AID - molecules-31-03624 [pii] AID - 10.3390/molecules31203624 [doi] PST - epublish SO - Molecules. 2026 Oct 11;31(20):3624. doi: 10.3390/molecules31203624. PMID- 42943695 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 11 TI - Effects of Superfine Grinding on Physicochemical Properties and In Vitro Starch Digestibility of Red Rice Powder. LID - 10.3390/molecules31203622 [doi] LID - 3622 AB - Pigmented red rice is a promising functional whole grain, but conventional milling cannot fully release its intracellular bioactives due to dense bran cell walls. Superfine grinding can effectively destroy compact tissue structures and improve the processing characteristics of cereal powders. In this study, red rice powders (RRPs) with different particle sizes were prepared by superfine grinding, and their physicochemical properties, micromorphology, crystalline structure, total phenolic content (TPC), pasting behaviors, and in vitro starch digestibility were tested by particle size analysis, colorimetric measurement, SEM, XRD, FT-IR, the Folin-Ciocalteu method, RVA, and an in vitro simulated digestion model. The results show that superfine grinding caused severe matrix destruction, reducing the particle size (D90) by 83.5%, and remarkably increasing the specific surface area. Furthermore, superfine grinding improved color brightness, decreased powder flowability, and reduced starch crystallinity, while no new functional groups were generated. Additionally, the TPC and water/oil-holding capacity were effectively enhanced. The pasting properties and in vitro starch digestibility of RRP were modified by superfine grinding. This study provides basic data on RRP properties and recommends the selection of proper grinding intensity in order to obtain powder for diverse food applications, such as functional beverages and bakery products. FAU - Guo, Xiaojuan AU - Guo X AD - College of Chemistry and Food Science, Nanchang Normal University, Nanchang 330023, China. AD - Jiangxi Provincial Research Center for National Nutrition Health Education, Nanchang 330023, China. FAU - Shuai, Liang AU - Shuai L AUID- ORCID: 0000-0001-7652-4626 AD - College of Chemistry and Food Science, Nanchang Normal University, Nanchang 330023, China. AD - Jiangxi Provincial Research Center for National Nutrition Health Education, Nanchang 330023, China. LA - eng GR - GJJ2401907/Jiangxi Provincial Department of Education Science and Technology Research Youth Project/ GR - NSBSJJ2023011/Doctor Foundation of Nanchang Normal University/ GR - NCNUCXTD001/Nanchang Normal University Science and Technology Innovation Team/ PT - Journal Article DEP - 20261011 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 9005-25-8 (Starch) RN - 0 (Powders) SB - IM MH - *Oryza/chemistry MH - *Starch/chemistry MH - Powders/chemistry MH - Particle Size MH - Spectroscopy, Fourier Transform Infrared MH - *Food Handling/methods MH - Digestion MH - *Chemical Phenomena MH - X-Ray Diffraction PMC - PMC13707927 OTO - NOTNLM OT - physicochemical properties OT - red rice OT - starch digestibility OT - superfine grind COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/11 CRDT- 2026/10/27 10:46 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - molecules31203622 [pii] AID - molecules-31-03622 [pii] AID - 10.3390/molecules31203622 [doi] PST - epublish SO - Molecules. 2026 Oct 11;31(20):3622. doi: 10.3390/molecules31203622. PMID- 42943692 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 11 TI - Synthesis, Antioxidant Activity, In Silico Evaluation, and Cytotoxicity of Novel Polyphenolic-Methyliden-Hydrazono-Thiazole Derivatives. LID - 10.3390/molecules31203620 [doi] LID - 3620 AB - Novel thiazolyl-polyphenolic derivatives were designed and synthesized to investigate the influence of aromatic substituents on antioxidant activity, molecular properties, and cytotoxicity. Eight derivatives (E1-E8) were synthesized by a Hantzsch reaction and their structures were confirmed by MS, FT-IR, and (1)H/(13)C NMR spectroscopy. Antioxidant activity was evaluated using DPPH, ABTS, TAC, RP, FRAP, and CUPRAC assays. The experimental results were supported by DFT calculations and SwissADME prediction, while cytotoxicity was evaluated against A549 lung adenocarcinoma cells and normal BJ fibroblasts. The antioxidant activity depended on the nature of the aromatic substituent. Compound E5 showed a superior antioxidant profile, presenting the highest DPPH radical scavenging activity and the strongest reducing power in the RP assay. The theoretical calculations supported these findings, showing favorable HOMO energy and low O-H bond dissociation energy values. Compound E6 showed the highest activity in the CUPRAC assay and exhibited the strongest cytotoxic activity against A549 cells together with the highest selectivity index. All synthesized compounds complied with Lipinski's rule of five. Overall, the results show that the electronic properties of the aromatic substituent strongly influence both the antioxidant and cytotoxic activities of this series. These compounds may represent useful scaffolds for further development of antioxidant and anticancer agents. FAU - Cornea, Alexandra Catalina AU - Cornea AC AD - Department of Pharmaceutical Chemistry, "Iuliu Hatieganu" University of Medicine and Pharmacy, 41 Victor Babes Street, 400012 Cluj-Napoca, Romania. FAU - Marc, Gabriel AU - Marc G AUID- ORCID: 0000-0001-9397-0388 AD - Department of Organic Chemistry, "Iuliu Hatieganu" University of Medicine and Pharmacy, 41 Victor Babes Street, 400012 Cluj-Napoca, Romania. FAU - Fizesan, Ionel AU - Fizesan I AD - Department of Toxicology, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, 8 Victor Babes, 400012 Cluj-Napoca, Romania. FAU - Ionut, Ioana AU - Ionut I AD - Department of Pharmaceutical Chemistry, "Iuliu Hatieganu" University of Medicine and Pharmacy, 41 Victor Babes Street, 400012 Cluj-Napoca, Romania. FAU - Moldovan, Cristina AU - Moldovan C AUID- ORCID: 0000-0002-5803-6108 AD - Department of Pharmaceutical Chemistry, "Iuliu Hatieganu" University of Medicine and Pharmacy, 41 Victor Babes Street, 400012 Cluj-Napoca, Romania. FAU - Stana, Anca AU - Stana A AUID- ORCID: 0000-0002-8453-3642 AD - Department of Pharmaceutical Chemistry, "Iuliu Hatieganu" University of Medicine and Pharmacy, 41 Victor Babes Street, 400012 Cluj-Napoca, Romania. FAU - Tiperciuc, Brindusa AU - Tiperciuc B AUID- ORCID: 0000-0002-1333-5649 AD - Department of Pharmaceutical Chemistry, "Iuliu Hatieganu" University of Medicine and Pharmacy, 41 Victor Babes Street, 400012 Cluj-Napoca, Romania. FAU - Crestin, Ionut-Valentin AU - Crestin IV AUID- ORCID: 0009-0003-7144-0847 AD - Department of Toxicology, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, 8 Victor Babes, 400012 Cluj-Napoca, Romania. FAU - Pirnau, Adrian AU - Pirnau A AUID- ORCID: 0000-0002-2313-9350 AD - National Institute for Research and Development of Isotopic and Molecular Technologies, 67-103 Donath Street, 400293 Cluj-Napoca, Romania. FAU - Vlase, Laurian AU - Vlase L AUID- ORCID: 0000-0002-0664-3387 AD - Department of Pharmaceutical Technology and Biopharmaceutics, "Iuliu Hatieganu" University of Medicine and Pharmacy, 41 Victor Babes Street, 400012 Cluj-Napoca, Romania. FAU - Oniga, Ilioara AU - Oniga I AUID- ORCID: 0000-0002-7660-9993 AD - Department of Pharmacognosy, "Iuliu Hatieganu" University of Medicine and Pharmacy, 12 Ion Creanga Street, 400010 Cluj-Napoca, Romania. FAU - Oniga, Ovidiu AU - Oniga O AD - Department of Pharmaceutical Chemistry, "Iuliu Hatieganu" University of Medicine and Pharmacy, 41 Victor Babes Street, 400012 Cluj-Napoca, Romania. LA - eng GR - 27N/03.01.2023/MEC of Romania/ GR - PN 23 24 01 05/MEC of Romania/ GR - 771/17/11.01.2023, 647/17/11.01.2024, 776/16/13.01.2025/Iuliu Hatieganu University of Medicine and Pharmacy/ PT - Journal Article DEP - 20261011 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Antioxidants) RN - 0 (Thiazoles) RN - 0 (Polyphenols) RN - 0 (Antineoplastic Agents) SB - IM MH - Humans MH - *Antioxidants/pharmacology/chemical synthesis/chemistry MH - *Thiazoles/chemistry/pharmacology/chemical synthesis MH - *Polyphenols/chemistry/pharmacology/chemical synthesis MH - A549 Cells MH - *Antineoplastic Agents/pharmacology/chemical synthesis/chemistry MH - Structure-Activity Relationship MH - Molecular Structure MH - Computer Simulation MH - Cell Survival/drug effects PMC - PMC13707546 OTO - NOTNLM OT - DFT OT - ROS OT - anticancer OT - antioxidant OT - phenol OT - thiazole COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/11 CRDT- 2026/10/27 10:46 PHST- 2026/08/05 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - molecules31203620 [pii] AID - molecules-31-03620 [pii] AID - 10.3390/molecules31203620 [doi] PST - epublish SO - Molecules. 2026 Oct 11;31(20):3620. doi: 10.3390/molecules31203620. PMID- 42943690 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 11 TI - Berry Anthocyanins and Their Metabolites in Endothelial Dysfunction Associated with Diabetes and Cardiometabolic Risk. LID - 10.3390/molecules31203618 [doi] LID - 3618 AB - Diabetes-related endothelial dysfunction involves oxidative stress, impaired nitric oxide (NO) signaling, and inflammation. Because ingested berry anthocyanins undergo extensive metabolism, circulating conjugates and phenolic products may contribute to vascular responses. This critical narrative review searched MEDLINE/PubMed, Web of Science, and Scopus through 1 July 2026 and examined citation trails. Among 298 database records, 253 were unique. The screening log selected 29 reports for full-text assessment; 26 were included and three excluded after full-text analysis. Four reports entered through citation tracking. The 30 included reports comprised eight cell-based, twelve animal or mixed, and ten human reports; two human reports analyzed related participants. Experimental studies associated berry preparations or metabolites with lower measured reactive oxygen species, NADPH oxidase (NOX) expression, and inflammatory markers, sometimes alongside preserved NO signaling or vascular relaxation. Expression changes do not establish NOX inhibition or nuclear factor kappa B (NF-kappaB)-dependent mediation. Several small phenolic products lack anthocyanin-specific provenance. Human vascular findings in cardiometabolic risk populations were mixed, and no included berry intervention in established diabetes used a direct endothelial endpoint. Current evidence supports mechanistic plausibility but does not establish clinical efficacy in diabetes or attribute whole-berry effects to anthocyanins alone. FAU - Zujko, Malgorzata Elzbieta AU - Zujko ME AUID- ORCID: 0000-0002-0410-3539 AD - Department of Food Biotechnology, Faculty of Health Sciences, Medical University of Bialystok, Szpitalna 37, 15-295 Bialystok, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261011 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Anthocyanins) SB - IM MH - Humans MH - *Anthocyanins/metabolism/pharmacology/chemistry MH - Animals MH - *Fruit/chemistry/metabolism MH - *Endothelium, Vascular/metabolism/drug effects/physiopathology MH - *Diabetes Mellitus/metabolism MH - Oxidative Stress/drug effects MH - Cardiometabolic Risk Factors PMC - PMC13707516 OTO - NOTNLM OT - anthocyanins OT - berries OT - diabetes mellitus OT - endothelial dysfunction OT - phenolic metabolites COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:06 PMCR- 2026/10/11 CRDT- 2026/10/27 10:46 PHST- 2026/09/23 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:06 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:46 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - molecules31203618 [pii] AID - molecules-31-03618 [pii] AID - 10.3390/molecules31203618 [doi] PST - epublish SO - Molecules. 2026 Oct 11;31(20):3618. doi: 10.3390/molecules31203618. PMID- 42943687 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 31 IP - 20 DP - 2026 Oct 10 TI - Multibatch Analysis of Process Sensitivity Across Stages in 100 g Scale Mannose Triflate Synthesis. LID - 10.3390/molecules31203616 [doi] LID - 3616 AB - Mannose triflate is an established precursor for nucleophilic 2-deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG) production, but same-scale, stage-resolved process behavior is incompletely documented. This retrospective study combines three 100 g D-mannose confirmation batches with exploratory process-deviation observations. Stage III (orthoester isolation), Stage IV (selective hydrolysis), and Stage V (triflation) showed recorded yield relative standard deviations (RSDs) of 9.7%, 14.5%, and 2.0%, respectively. Recovery of contemporaneous process-validation and research and development (R&D) records provided direct batch-specific Stage III outputs and Stage IV/V charges and outputs, resolving the principal yield-denominator ambiguity; one archived Stage IV-to-V carry-forward mismatch in MT-09.06.004 remains explicitly unresolved. Elevated Stage IV evaporation temperature (20/36 degrees C versus 98%). Filtration and the combined method received higher usability scores than centrifugation, whereas the combined workflow required approximately 15% higher consumable costs. Storage effects were target-, fraction-, and temperature-dependent. No common holding time satisfied the complete two-sided operational acceptance criteria. Considering decreases in relative recovery alone, storage at 5 degrees C for up to six days did not reduce any mean recovery below its target- and fraction-specific lower limit. Storage at -20 degrees C produced decreases in iDNA recovery after 24 h. These findings offer practical guidance for PCR-based AMR monitoring in wastewater. FAU - Boeker-Haeusser, Sarah AU - Boeker-Haeusser S AUID- ORCID: 0000-0003-3910-0845 AD - Hamm-Lippstadt University of Applied Sciences, 59063 Hamm, Germany. AD - Bauhaus-Institute for Infrastructure Solutions (b.is), Bauhaus University Weimar, 99423 Weimar, Germany. FAU - Wrede, Hanna AU - Wrede H AD - Hamm-Lippstadt University of Applied Sciences, 59063 Hamm, Germany. FAU - Klumper, Claudia AU - Klumper C AUID- ORCID: 0000-0002-3419-7855 AD - Hamm-Lippstadt University of Applied Sciences, 59063 Hamm, Germany. FAU - Beier, Silvio AU - Beier S AD - Bauhaus-Institute for Infrastructure Solutions (b.is), Bauhaus University Weimar, 99423 Weimar, Germany. LA - eng PT - Journal Article DEP - 20261010 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707453 OTO - NOTNLM OT - PCR OT - antibiotic resistance genes OT - antimicrobial resistance OT - extracellular DNA OT - intracellular DNA OT - sample preparation OT - sample storage OT - wastewater OT - wastewater monitoring COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:45 PHST- 2026/06/26 00:00 [received] PHST- 2026/09/06 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - microorganisms14102316 [pii] AID - microorganisms-14-02316 [pii] AID - 10.3390/microorganisms14102316 [doi] PST - epublish SO - Microorganisms. 2026 Oct 10;14(10):2316. doi: 10.3390/microorganisms14102316. PMID- 42943544 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 10 TI - Isolation, Identification and Genomic Characterization of Carbamazepine-Tolerant Bacteria from Contaminated Aquatic Environments. LID - 10.3390/microorganisms14102305 [doi] LID - 2305 AB - Pharmaceutical contamination in aquatic environments has increased interest in microorganisms able to tolerating these compounds and the mechanisms underlying their tolerance. This study isolated and characterized carbamazepine (CBZ)-tolerant bacteria from contaminated waters of the Machangara River, Quito, Ecuador, and investigated the genome of the most tolerant isolate. Isolates were characterized by morphological and biochemical analyses, and 16S rRNA gene sequencing. Tolerance to CBZ (1-50 mg L(-1)) was evaluated by culture-based growth measurements. CBZ1, CBZ3, CBZ6, and CBZ7 were taxonomically assigned to Enterobacter hormaechei, Paenibacillus lactis, Aeromonas hydrophila and Raoultella ornithinolytica, respectively. Among the four isolates evaluated, CBZ7 exhibited significantly higher relative growth at elevated CBZ concentrations (30-50 mg L(-1)) and was therefore selected for whole-genome sequencing. The CBZ7 draft genome comprised 5,671,759 bp distributed across 181 contigs, with a G+C content of 55.65%, an N50 of 79,780 bp, 98.2% BUSCO completeness, and CheckM2 estimates 0.87% contamination. Genome annotation revealed genes associated with membrane transport and efflux, oxidative metabolism, glutathione metabolism, environmental sensing, and aromatic compound metabolism. Although no complete CBZ-specific catabolic pathway was identified, the results support strong tolerance of CBZ7 to elevated CBZ concentrations and identity candidate functions potentially involved in xenobiotic adaptation. FAU - Mendez, Gabriela AU - Mendez G AUID- ORCID: 0000-0001-5903-6735 AD - Carrera de Biotecnologia, Universidad Politecnica Salesiana, Sede Quito, Campus El Giron, Av. 12 de Octubre N2422 y Wilson, Quito 170143, Ecuador. FAU - Carrera, Diana AU - Carrera D AD - Carrera de Biotecnologia, Universidad Politecnica Salesiana, Sede Quito, Campus El Giron, Av. 12 de Octubre N2422 y Wilson, Quito 170143, Ecuador. FAU - Olivo Silva, Lizbeth AU - Olivo Silva L AD - Master Innovative Drugs Parcours Nanomedicine, Universite Marie et Louis Pasteur (UMLP), Site Besancon, 1 rue Claude Goudimel, 25030 Besancon Cedex, France. FAU - Coyago-Cruz, Elena AU - Coyago-Cruz E AUID- ORCID: 0000-0003-4495-3500 AD - Carrera de Biotecnologia, Universidad Politecnica Salesiana, Sede Quito, Campus El Giron, Av. 12 de Octubre N2422 y Wilson, Quito 170143, Ecuador. FAU - Ortiz, Melisa AU - Ortiz M AD - Independent Researcher, Quito 170517, Ecuador. FAU - Vega, Ivonne AU - Vega I AD - Carrera de Biotecnologia, Universidad Politecnica Salesiana, Sede Quito, Campus El Giron, Av. 12 de Octubre N2422 y Wilson, Quito 170143, Ecuador. LA - eng GR - Universidad Politecnica Salesiana/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707586 OTO - NOTNLM OT - 16 S rRNA gene OT - Raoultella ornithinolytica OT - aromatic-compound metabolism OT - bacterial tolerance OT - carbamazepine OT - environmental bacteria OT - whole-genome sequencing OT - xenobiotic metabolism COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:45 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - microorganisms14102305 [pii] AID - microorganisms-14-02305 [pii] AID - 10.3390/microorganisms14102305 [doi] PST - epublish SO - Microorganisms. 2026 Oct 10;14(10):2305. doi: 10.3390/microorganisms14102305. PMID- 42943541 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 9 TI - Contrasting Bacterial and Fungal Assemblages Across Three Co-Flowering Exotic Plant Species Visited by Stingless Bees in Bali, Indonesia. LID - 10.3390/microorganisms14102302 [doi] LID - 2302 AB - Flowers are transient, chemically selective habitats in which microbial communities assemble rapidly and can influence nectar chemistry and pollinator behavior, yet the bacterial and fungal assemblages of tropical, bee-foraged plants remain largely uncharacterized and are seldom examined in parallel. We characterized whole-flower bacterial and fungal microbiota of three co-flowering plant species, Antigonon leptopus, Coffea arabica and Xanthostemon chrysanthus, growing at a single site in Bali, Indonesia, and visited by the stingless bee Tetragonula laeviceps, using paired 16S rRNA and ITS2 amplicon profiling. The three composite samples differed markedly in composition, with fewer than four percent of taxa shared among all three plants despite close spatial proximity and shared climate. Bacterial communities were dominated by Proteobacteria and fungal communities were dominated by Ascomycota and included lineages characteristic of nectar and of bee-associated environments, notably Asaia and other Acetobacteraceae, Pantoea and Rosenbergiella. The two markers did not order the hosts congruently in the samples analyzed: bacterial communities grouped A. leptopus with C. arabica, whereas fungal communities grouped it with X. chrysanthus, a decoupling consistent across clustering, taxon sharing and diversity ranking. These findings indicate that these floral communities differed markedly despite close spatial proximity and shared climate and suggest possible cross-kingdom differences in community organization that warrant examination in replicated designs. FAU - Pilianto, Joko AU - Pilianto J AD - College of Plant Protection, Northwest A&F University, Yangling 712100, China. AD - Department of Plant Pests and Diseases, Faculty of Bio-industry, Agriculture and Forestry, Universitas Brawijaya, Jl. Veteran, Malang 65145, Indonesia. FAU - Xiao, Yao AU - Xiao Y AD - College of Plant Protection, Northwest A&F University, Yangling 712100, China. FAU - Hadi, Mochammad Syamsul AU - Hadi MS AUID- ORCID: 0000-0003-4488-8788 AD - Department of Plant Pests and Diseases, Faculty of Bio-industry, Agriculture and Forestry, Universitas Brawijaya, Jl. Veteran, Malang 65145, Indonesia. FAU - Kusuma, Restu Rizkyta AU - Kusuma RR AD - Department of Plant Pests and Diseases, Faculty of Bio-industry, Agriculture and Forestry, Universitas Brawijaya, Jl. Veteran, Malang 65145, Indonesia. FAU - Widjayanti, Tita AU - Widjayanti T AD - The United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan. FAU - Abou El-Ela, Amr AU - Abou El-Ela A AUID- ORCID: 0000-0003-2195-4498 AD - State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. AD - Plant Protection Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Egypt. AD - Shandong Lushou Seed Industry Co., Ltd., Shouguang 262700, China. FAU - Alam, Syed Shane AU - Alam SS AD - College of Plant Protection, Northwest A&F University, Yangling 712100, China. FAU - TabinKhalkha, Surnaa AU - TabinKhalkha S AD - College of Plant Protection, Northwest A&F University, Yangling 712100, China. FAU - Shah, Kaleem Ullah AU - Shah KU AD - College of Plant Protection, Northwest A&F University, Yangling 712100, China. FAU - Aly, Hamdy H AU - Aly HH AUID- ORCID: 0009-0002-2278-0039 AD - College of Plant Protection, Northwest A&F University, Yangling 712100, China. FAU - Yu, Xiao AU - Yu X AUID- ORCID: 0009-0004-0788-7756 AD - College of Plant Protection, Northwest A&F University, Yangling 712100, China. FAU - Muyasarah, Kamila AU - Muyasarah K AD - Harvard Medical School, Harvard University, Boston, MA 02115, USA. FAU - Munawar, Asim AU - Munawar A AUID- ORCID: 0000-0002-9101-9545 AD - State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. FAU - Zhou, Wenwu AU - Zhou W AD - State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. FAU - Zhu, Zengrong AU - Zhu Z AUID- ORCID: 0000-0002-3247-1486 AD - State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. FAU - Wang, Dun AU - Wang D AUID- ORCID: 0000-0001-5762-5560 AD - College of Plant Protection, Northwest A&F University, Yangling 712100, China. LA - eng GR - 2022YFD1401001/National Key R&D Program of China/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707677 OTO - NOTNLM OT - 16S rRNA gene OT - ITS2 OT - Indonesia OT - bacterial-fungal comparison OT - co-flowering plants OT - floral microbiome OT - host specificity OT - host-associated variation COIS- Author Amr Abou El-Ela was employed by the company Shandong Lushou Seed Industry Co., Ltd., Shouguang, China. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:45 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - microorganisms14102302 [pii] AID - microorganisms-14-02302 [pii] AID - 10.3390/microorganisms14102302 [doi] PST - epublish SO - Microorganisms. 2026 Oct 9;14(10):2302. doi: 10.3390/microorganisms14102302. PMID- 42943534 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 9 TI - Species-Dependent Effects of 3-Hydroxychromone Derivatives on Biofilm Biomass and Planktonic Growth. LID - 10.3390/microorganisms14102295 [doi] LID - 2295 AB - A series of 3-hydroxychromone derivatives bearing Mannich-type motifs was synthesized and evaluated for effects on biofilm-associated biomass and planktonic growth in Staphylococcus aureus ALC2085 and Pseudomonas aeruginosa PAO1. Compounds were initially screened at 20 mug/mL using crystal violet staining, followed by experimental 50% growth-inhibitory concentration (GIC(50)) and CFU analyses. Against S. aureus, aryl-substituted derivatives had GIC(50) values >200 mug/mL, whereas 6-bromo derivatives had values of 1.25-10 mug/mL. Thus, the biomass reductions produced by NSK 18 at 20 mug/mL could not be separated from planktonic growth inhibition. Against PAO1, NSK 16 produced statistically significant decreases in biomass, reducing it to 50 +/- 24% of the untreated control (p = 0.03584), without comparably large or sustained reductions in the measured planktonic-growth endpoints at 20 mug/mL. These findings demonstrate species- and substitution-dependent activity and identify NSK 16 as preliminary PAO1 biomass-reducing hits. Because biomass was measured at a single concentration and not normalized to growth under matched conditions, further concentration-response and viability studies are required to establish biofilm selectivity. FAU - Wouna, Safi Dapetel Balkissou AU - Wouna SDB AUID- ORCID: 0009-0007-8843-3909 AD - Chemistry, Chemical and Biomedical Engineering, University of New Haven, West Haven, CT 06516, USA. FAU - Kolli, Naga Sai AU - Kolli NS AD - Chemistry, Chemical and Biomedical Engineering, University of New Haven, West Haven, CT 06516, USA. FAU - Benes, Kaitlyn AU - Benes K AD - Chemistry, Chemical and Biomedical Engineering, University of New Haven, West Haven, CT 06516, USA. FAU - Fauver, Devin AU - Fauver D AUID- ORCID: 0009-0006-5734-9344 AD - Chemistry, Chemical and Biomedical Engineering, University of New Haven, West Haven, CT 06516, USA. FAU - L Salzano, Carly AU - L Salzano C AD - Chemistry, Chemical and Biomedical Engineering, University of New Haven, West Haven, CT 06516, USA. FAU - Parsha, Rohini AU - Parsha R AD - Chemistry, Chemical and Biomedical Engineering, University of New Haven, West Haven, CT 06516, USA. FAU - Prajapati, Simran AU - Prajapati S AD - Chemistry, Chemical and Biomedical Engineering, University of New Haven, West Haven, CT 06516, USA. FAU - Cirillo, Pier F AU - Cirillo PF AUID- ORCID: 0000-0002-1458-5674 AD - Chemistry, Chemical and Biomedical Engineering, University of New Haven, West Haven, CT 06516, USA. FAU - Gu, Huan AU - Gu H AD - Chemistry, Chemical and Biomedical Engineering, University of New Haven, West Haven, CT 06516, USA. LA - eng GR - NSF: 2418289/National Science Foundation/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707711 OTO - NOTNLM OT - 3-hydroxychromone scaffold OT - 50% growth inhibition OT - GIC50 OT - Pseudomonas aeruginosa OT - Staphylococcus aureus OT - antibiofilm activity OT - structure-activity relationship COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:45 PHST- 2026/08/20 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - microorganisms14102295 [pii] AID - microorganisms-14-02295 [pii] AID - 10.3390/microorganisms14102295 [doi] PST - epublish SO - Microorganisms. 2026 Oct 9;14(10):2295. doi: 10.3390/microorganisms14102295. PMID- 42943528 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 8 TI - Emergence of NDM-5-Positive Salmonella enterica Serovar Typhimurium Monophasic Variant ST34 in Hong Kong During 2020-2025. LID - 10.3390/microorganisms14102289 [doi] LID - 2289 AB - The emergence of carbapenem-resistant non-typhoidal Salmonella species (CR-NTS) has been observed in recent years, causing increasing challenges in the clinical management and control of Salmonella infections. However, CR-NTS had not been comprehensively studied in Hong Kong previously. We retrieved archived non-typhoidal Salmonella isolates demonstrating carbapenem resistance from five public hospitals in Hong Kong during January 2020-June 2025. The isolates were characterised by NG-TEST CARBA-5 and subjected to whole-genome sequencing (WGS) and phylogenetic analysis. Clinical information of CR-NTS cases was also reviewed. Fourteen CR-NTS isolates (0.3%, 14/4404) were identified during the study period. All 13 CR-NTS isolates (one isolate could not be recovered) were NDM (New Delhi metallo-beta-lactamase)-positive, with the first case identified in March 2023. WGS revealed six Group B Salmonella monophasic variants of Salmonella enterica serovar Typhimurium ST34 harbouring the bla(NDM-5) gene. The most common plasmid replicon co-present among bla(NDM-5)-positive isolates was IncHI2-IncHI2A (63.6%, 7/11). Among cases of CR-NTS in our study, self-limiting gastroenteritis was the most common presentation (84.6%, 11/13); none presented with invasive infections; travel history was present in 7.7% (1/13). Phylogenetic analysis showed the genotypic relatedness of the ST34 isolates identified in Hong Kong to those on the Chinese mainland, with the possibility of local CR-NTS acquisition via imported food sources. Our findings highlighted the importance of a One Health approach to the control of CR-NTS and the need for continuous antimicrobial resistance surveillance of Salmonella spp. in Hong Kong. FAU - Tse, Atalie Ching-Yee AU - Tse AC AUID- ORCID: 0009-0004-1447-0043 AD - Department of Pathology, Queen Elizabeth Hospital, Hong Kong SAR, China. AD - Department of Pathology, Hong Kong Children's Hospital, Hong Kong SAR, China. FAU - Choi, Garnet Kwan-Yue AU - Choi GK AD - Department of Pathology, Hong Kong Children's Hospital, Hong Kong SAR, China. FAU - Luk, Hayes Kam-Hei AU - Luk HK AUID- ORCID: 0000-0002-9831-2912 AD - Department of Pathology, Queen Elizabeth Hospital, Hong Kong SAR, China. FAU - Yip, Eric Kam-Tong AU - Yip EK AD - Department of Clinical Pathology, Tuen Mun Hospital, Hong Kong SAR, China. FAU - Cheng, Chi-Ka AU - Cheng CK AD - Department of Pathology, United Christian Hospital, Hong Kong SAR, China. FAU - Chau, Sandy Ka-Yee AU - Chau SK AUID- ORCID: 0000-0003-0330-976X AD - Department of Pathology, United Christian Hospital, Hong Kong SAR, China. FAU - Ng, Ho-Leung AU - Ng HL AD - Department of Clinical Pathology, Tuen Mun Hospital, Hong Kong SAR, China. FAU - Tse, Herman AU - Tse H AD - Department of Laboratory Medicine, Khoo Teck Puat Hospital, Singapore 768828, Singapore. FAU - Wong, Sally Cheuk-Ying AU - Wong SC AUID- ORCID: 0000-0003-2126-6484 AD - Department of Pathology, Queen Elizabeth Hospital, Hong Kong SAR, China. AD - Department of Pathology, Hong Kong Children's Hospital, Hong Kong SAR, China. FAU - Lung, David Christopher AU - Lung DC AUID- ORCID: 0000-0001-6047-9744 AD - Department of Pathology, Queen Elizabeth Hospital, Hong Kong SAR, China. AD - Department of Pathology, Hong Kong Children's Hospital, Hong Kong SAR, China. LA - eng PT - Journal Article DEP - 20261008 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707440 OTO - NOTNLM OT - ST34 OT - blaNDM-5 gene OT - carbapenem OT - multidrug resistance OT - non-typhoidal Salmonella spp. COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:45 PHST- 2026/08/22 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - microorganisms14102289 [pii] AID - microorganisms-14-02289 [pii] AID - 10.3390/microorganisms14102289 [doi] PST - epublish SO - Microorganisms. 2026 Oct 8;14(10):2289. doi: 10.3390/microorganisms14102289. PMID- 42943527 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 8 TI - Fermentation and Refrigerated Storage of Millet-Based Beverages with Yoghurt and Propionic Acid Bacteria. LID - 10.3390/microorganisms14102288 [doi] LID - 2288 AB - Millet beverages require fermentation processes that provide acidification, culturable starter populations and resistance to serum separation. Beverages containing 0 to 3% (w/v) millet flour were fermented with a yoghurt starter, Propionibacterium freudenreichii subsp. shermanii, or both cultures. Fermentation was conducted at 37 degrees C for 6 h with yoghurt or mixed cultures and 18 h with the propionic starter, followed by storage at 6 degrees C for 28 days. Two independent production experiments were performed, each with duplicate samples. Acidification, bacterial counts, pH, penetration forces, centrifugal syneresis and selected carbohydrates were assessed. Mixed cultures yielded higher presumptive propionibacteria counts after fermentation and storage, reaching 6.7 log CFU/mL on day 28 with 2% flour. Their pH remained approximately 4.1-4.2. Flour increased hardness and reduced centrifugal serum release. With 3% flour, syneresis on day 28 was 48.3% for yoghurt and 57.8% for mixed cultures. Carbohydrate concentrations below 0.50 mg/g were treated as semiquantitative estimates. The results describe formulation-dependent technological responses without establishing metabolic synergy. Limited independent replication, substantial centrifugal serum release and the absence of rheological and sensory measurements restrict statistical inference and conclusions about commercial suitability. FAU - Ziarno, Malgorzata AU - Ziarno M AUID- ORCID: 0000-0001-7445-6375 AD - Department of Food Technology and Assessment, Faculty of Bioeconomy and Food Systems, Warsaw University of Life Sciences-SGGW (WULS-SGGW), Nowoursynowska 159c St., 02-776 Warsaw, Poland. FAU - Zareba, Dorota AU - Zareba D AD - Professor E. Pijanowski Catering School Complex in Warsaw, 04-110 Warsaw, Poland. FAU - Hasalliu, Rozeta AU - Hasalliu R AUID- ORCID: 0000-0001-8729-6251 AD - Department of Food Science and Biotechnology, Faculty of Food Biotechnology, Agricultural University of Tirana, Rruga "Pajsi Vodica", Koder-Kamez, 1029 Tirana, Albania. LA - eng PT - Journal Article DEP - 20261008 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707843 OTO - NOTNLM OT - Propionibacterium freudenreichii OT - co-fermentation OT - microbial viability OT - millet beverage OT - plant-based fermentation OT - refrigerated storage OT - syneresis OT - yoghurt starter culture COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:45 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - microorganisms14102288 [pii] AID - microorganisms-14-02288 [pii] AID - 10.3390/microorganisms14102288 [doi] PST - epublish SO - Microorganisms. 2026 Oct 8;14(10):2288. doi: 10.3390/microorganisms14102288. PMID- 42943525 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 8 TI - The Species-Selective Activity of Peptiskin Is Associated with Cell Wall Affinity and Extracellular DNA Binding in Staphylococcus aureus and Staphylococcus epidermidis. LID - 10.3390/microorganisms14102287 [doi] LID - 2287 AB - Skin health depends on the balance of the commensal microbiota. Excessive proliferation of Staphylococcus aureus contributes to inflammatory skin diseases, such as atopic dermatitis, whereas Staphylococcus epidermidis helps maintain skin homeostasis. We previously reported that Peptiskin, a mixture of short-chain cationic oligopeptides containing arginine and lysine, selectively suppresses S. aureus while promoting S. epidermidis growth, although the underlying mechanism remains unclear. We investigated this mechanism by evaluating Peptiskin's effects on pH, nutrient utilization, extracellular DNA (eDNA), membrane damage, cell wall binding, antibacterial activity, bacteriostatic activity, and intracellular gene expression. The species-specific effects were not fully explained by changes in pH or nutrient availability. In S. aureus, Peptiskin induced substantial membrane damage, as indicated by ~66% lactate dehydrogenase release, stronger cell wall binding, higher antibacterial activity, and reduced ATP synthase-related gene expression, consistent with bactericidal and bacteriostatic effects. Conversely, membrane damage was minimal in S. epidermidis, whereas Peptiskin showed distinct interactions with eDNA, and extracellular interactions may contribute to the distinct growth response. The species-specific activity of Peptiskin was associated with differences in cell wall affinity, eDNA interactions, membrane susceptibility, and intrinsic physiological responses, providing a potential strategy for selectively modulating the skin microbiota while suppressing S. aureus-dominant conditions. FAU - Michihara, Akihiro AU - Michihara A AD - Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, Hiroshima 729-0292, Japan. FAU - Tange, Masato AU - Tange M AD - Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, Hiroshima 729-0292, Japan. FAU - Matsuoka, Hiroshi AU - Matsuoka H AUID- ORCID: 0000-0001-9423-5473 AD - Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, Hiroshima 729-0292, Japan. FAU - Wang, Fangfang AU - Wang F AD - Beauty Hi-tech Innovation Co., Ltd., Kobe International Business Center 605, Chuo-ku, Kobe, Hyogo 650-0047, Japan. FAU - Lin, Xianting AU - Lin X AUID- ORCID: 0009-0003-2329-956X AD - Beauty Hi-tech Innovation Co., Ltd., Kobe International Business Center 605, Chuo-ku, Kobe, Hyogo 650-0047, Japan. FAU - Yang, Jianzhong AU - Yang J AUID- ORCID: 0000-0003-1935-8283 AD - Beauty Hi-tech Innovation Co., Ltd., Kobe International Business Center 605, Chuo-ku, Kobe, Hyogo 650-0047, Japan. LA - eng GR - Beauty Hi-tech Innovation Co., Ltd./ PT - Journal Article DEP - 20261008 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707529 OTO - NOTNLM OT - Peptiskin OT - Staphylococcus aureus OT - Staphylococcus epidermidis OT - extracellular DNA OT - skin microbiota regulation COIS- F.W., X.L. and J.Y. are employees of Beauty Hi-tech Innovation Co., Ltd., which provided funding for this study. F.W., X.L. and J.Y. contributed to the study as described in the Author Contributions section. A.M., H.M., F.W., X.L., and J.Y. are co-inventors of a pending patent application jointly filed by Fukuyama University and Beauty Hi-tech Innovation Co., Ltd. related to the technology investigated in this study. The authors declare no other conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:45 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - microorganisms14102287 [pii] AID - microorganisms-14-02287 [pii] AID - 10.3390/microorganisms14102287 [doi] PST - epublish SO - Microorganisms. 2026 Oct 8;14(10):2287. doi: 10.3390/microorganisms14102287. PMID- 42943522 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 8 TI - Genomic Characterization of Clinical Group B Streptococcus Isolates from Zhejiang, China: Serotypes, Clonal Complexes, and Predicted Antimicrobial Resistance and Virulence Gene Profiles. LID - 10.3390/microorganisms14102284 [doi] LID - 2284 AB - This study aimed to characterize the population structure, predicted antimicrobial resistance gene profiles, and virulence gene repertoire of Group B Streptococcus (GBS) in Zhejiang Province, China, using whole-genome sequencing (WGS). Forty GBS isolates from clinical specimens underwent WGS, multilocus sequence typing (MLST), cgMLST-based phylogenetic analysis, and systematic in silico annotation of predicted antimicrobial resistance and virulence genes. Seven capsular serotypes were identified, with serotype V being predominant. Fifteen sequence types (STs) were detected, including two novel STs (ST2662 and ST2663). Thirteen STs were grouped into eight clonal complexes (CCs), while two STs remained unassigned to any CC. CC19 (11/40, 27.5%) and CC327 (10/40, 25.0%) were the predominant CCs, with CC327 comprising the distinctive ST529-CC327-V clone. Eighteen predicted antimicrobial resistance genes were identified, including genes predicted to confer resistance to macrolide (ermB 60.0%, mefA 37.5%), tetracycline (tetM 47.5%, tetO 42.5%), aminoglycoside (aph(3')-IIIa 25.0%), and lincosamide (lsaE 30.0%, lnuB 30.0%). All isolates carried multiple predicted virulence genes, with universal carriage of adhesion genes fbsA and fbsB, and the invasion genes cylE, hylB, and cfb. These findings reveal a genetically diverse GBS population with distinctive clonal architecture and high carriage of predicted antimicrobial resistance genes in Zhejiang Province, underscoring the need for continued genomic surveillance to inform region-specific antibiotic stewardship and vaccine development. FAU - Yang, Zhangnv AU - Yang Z AUID- ORCID: 0000-0003-4740-8807 AD - Key Lab of Vaccine, Prevention and Control of Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310015, China. FAU - Jin, Zi AU - Jin Z AD - Key Lab of Vaccine, Prevention and Control of Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310015, China. FAU - Wang, Lingbo AU - Wang L AD - Key Lab of Vaccine, Prevention and Control of Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310015, China. FAU - Shi, Guoxiang AU - Shi G AD - Key Lab of Vaccine, Prevention and Control of Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310015, China. FAU - Yao, Wenwu AU - Yao W AUID- ORCID: 0009-0006-3816-0079 AD - Key Lab of Vaccine, Prevention and Control of Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310015, China. FAU - Gong, Xiaowei AU - Gong X AD - Key Lab of Vaccine, Prevention and Control of Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310015, China. FAU - Wu, Beibei AU - Wu B AD - Key Lab of Vaccine, Prevention and Control of Infectious Disease of Zhejiang Province, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310015, China. FAU - Hu, Weilin AU - Hu W AD - Department of Microbiology, Zhejiang University School of Medicine, Hangzhou 310058, China. LA - eng GR - 2023YFC2605104 and 2023YFC2605100/China National Center for Biotechnology Development/ GR - 2023ZL352/Zhejiang Traditional Chinese Medicine Administration/ GR - 2024KY894/Medical Science and Technology Program of Zhejiang Province/ GR - 2024C03216/Zhejiang Provincial Department of Science and Technology/ PT - Journal Article DEP - 20261008 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707904 OTO - NOTNLM OT - GBS OT - WGS OT - resistance genes OT - virulence genes COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:45 PHST- 2026/07/27 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - microorganisms14102284 [pii] AID - microorganisms-14-02284 [pii] AID - 10.3390/microorganisms14102284 [doi] PST - epublish SO - Microorganisms. 2026 Oct 8;14(10):2284. doi: 10.3390/microorganisms14102284. PMID- 42943508 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 6 TI - Changes in Antimicrobial Resistance in Bloodstream Infections During the COVID-19 Pandemic-A Retrospective Study from a Tertiary Care Hospital. LID - 10.3390/microorganisms14102269 [doi] LID - 2269 AB - The COVID-19 pandemic led to major repurposing of healthcare settings, especially Infectious Diseases hospitals, by redirecting care towards SARS-CoV-2-infected patients. However, frequent bacterial infections overlapping the viral background led to changes in antimicrobial resistance profiling among patients. This study aims to assess isolates from invasive infections and their antimicrobial susceptibility patterns in pre-pandemic and pandemic periods. Positive blood cultures from patients hospitalized in our Institute between January 2019 and September 2022 were assessed in terms of antimicrobial susceptibility, following sample processing as per laboratory protocol, with the use of the automated Bactalert((R))3D system, MALDI-TOF((R)) MS and microscopy for bacterial identification. Antimicrobial susceptibility testing was performed using the Vitek2Compact((R)) and Micronaut-S((R)) systems. Phenotypic enzyme production testing was assessed using the Resist-5 OKNVI kits. All data were subsequently digitalized, and statistical analysis was performed. A total of 932 non-duplicated bacterial and fungal strains were analyzed, with the most commonly isolated organisms being Staphylococcus aureus (n = 173), followed by Escherichia coli(n = 156), Acinetobacter baumannii (n = 95), and Klebsiella pneumoniae (n = 89). Resistance rates to multiple antimicrobials increased substantially amongst Klebsiella pneumoniae and Acinetobacter baumannii. Methicillin-resistant Staphylococcus aureus isolates followed a national decrease trend during the pandemic period; however, an increasingly resistant profile to macrolides may be attributable to the excessive and often unjustified use of Azithromycin during the pandemic period. The COVID-19 pandemic was accompanied by a shift in bacterial profile of invasive infections amongst our cohort, illustrating the dynamic nature of antimicrobial resistance in an Infectious Diseases hospital during those times, underscoring the complex interplay between the COVID-19 pandemic and antibiotic resistance patterns, and highlighting the need for ongoing surveillance and tailored antimicrobial stewardship efforts in clinical practice. FAU - Lumpan, Rodica Stela AU - Lumpan RS AUID- ORCID: 0009-0000-8149-306X AD - Faculty of Medicine, 'Carol Davila' University of Medicine and Pharmacy, 021021 Bucharest, Romania. AD - Ploiesti Pediatric Hospital, 100336 Ploiesti, Romania. FAU - Talapan, Daniela AU - Talapan D AUID- ORCID: 0000-0001-5202-8417 AD - Faculty of Medicine, 'Carol Davila' University of Medicine and Pharmacy, 021021 Bucharest, Romania. AD - 'Prof. Dr. Matei Bals' National Institute of Infectious Diseases, 021105 Bucharest, Romania. FAU - Borcan, Alina Maria AU - Borcan AM AUID- ORCID: 0009-0007-4208-6649 AD - Faculty of Medicine, 'Carol Davila' University of Medicine and Pharmacy, 021021 Bucharest, Romania. AD - 'Prof. Dr. Matei Bals' National Institute of Infectious Diseases, 021105 Bucharest, Romania. FAU - Stoica, Mihai AU - Stoica M AUID- ORCID: 0000-0003-1454-1425 AD - Faculty of Marketing, Bucharest University of Economic Studies, 010374 Bucharest, Romania. FAU - Dan, Mihai Octavian AU - Dan MO AUID- ORCID: 0000-0002-4908-5610 AD - Faculty of Medicine, 'Carol Davila' University of Medicine and Pharmacy, 021021 Bucharest, Romania. AD - 'Prof. Dr. Matei Bals' National Institute of Infectious Diseases, 021105 Bucharest, Romania. FAU - Rafila, Alexandru AU - Rafila A AD - Faculty of Medicine, 'Carol Davila' University of Medicine and Pharmacy, 021021 Bucharest, Romania. AD - 'Prof. Dr. Matei Bals' National Institute of Infectious Diseases, 021105 Bucharest, Romania. FAU - Arama, Victoria AU - Arama V AD - Faculty of Medicine, 'Carol Davila' University of Medicine and Pharmacy, 021021 Bucharest, Romania. AD - 'Prof. Dr. Matei Bals' National Institute of Infectious Diseases, 021105 Bucharest, Romania. LA - eng PT - Journal Article DEP - 20261006 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707747 OTO - NOTNLM OT - COVID-19 OT - antimicrobial resistance OT - blood cultures OT - carbapenem resistance OT - invasive infections COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/06 CRDT- 2026/10/27 10:45 PHST- 2026/07/31 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/06 00:00 [pmc-release] AID - microorganisms14102269 [pii] AID - microorganisms-14-02269 [pii] AID - 10.3390/microorganisms14102269 [doi] PST - epublish SO - Microorganisms. 2026 Oct 6;14(10):2269. doi: 10.3390/microorganisms14102269. PMID- 42943507 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 6 TI - Effects of Partial Replacement of Palm Oil with Schizochytrium Oil on Rumen Microbiota, Ruminal Volatile Fatty Acid Concentrations, Rumen Epithelial Transcriptome, and Serum Metabolome in Hu Lambs Fed a Medium-Fiber Diet. LID - 10.3390/microorganisms14102268 [doi] LID - 2268 AB - Schizochytrium oil (SO) is rich in docosahexaenoic acid (DHA), but its ruminal effects in sheep remain unclear. Twenty Hu lambs were fed CK or SO diets for 84 d. The CK diet contained 4% palm oil, 31.43% neutral detergent fiber (NDF), and 5.58% ether extract (EE). The SO diet contained 3% palm oil and 1% SO, with similar NDF (31.76%) and EE (5.45%). Blood samples were collected one day before slaughter, before the morning feed replenishment, whereas rumen contents and epithelial tissue were collected after slaughter following a 16-h fast. Rumen microbiota, volatile fatty acids (VFAs), epithelial morphology (n = 10/group), RNA-seq (n = 4/group), and serum metabolomics (n = 5/group) were analyzed. The results showed that the rumen microbial community structure did not differ between the two groups (PERMANOVA, R(2) = 0.101, p > 0.05), but SO selectively altered the relative abundance of seven genera (FDR < 0.05), including higher Fibrobacter and lower Methanosphaera. These changes were accompanied by higher acetate, propionate, butyrate, valerate, and total VFA concentrations and a lower acetate/propionate ratio (p < 0.05). Histological analysis showed that rumen papilla length and total epithelial thickness were higher in the SO group than in the CK group (2286.49 vs. 1764.30 mum and 114.52 vs. 80.07 mum, respectively; p < 0.05). Rumen epithelial RNA-seq showed enrichment of pathways related to VFA metabolism, energy metabolism, and fatty acid metabolism in the SO group (FDR < 0.01). Serum metabolomics showed higher levels of DHA and several DHA-containing lipids in the SO group (FDR < 0.05). Overall, partial replacement of palm oil with 1% SO selectively altered selected rumen genera and increased ruminal propionate concentration, accompanied by changes in rumen epithelial morphology and metabolism and increased serum DHA-related lipids. These findings support the potential of SO as a dietary DHA source for Hu lambs and provide evidence for its effects on the rumen and serum DHA-related lipid profile. FAU - Yao, Rongyu AU - Yao R AD - State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China. FAU - Wang, Ziang AU - Wang Z AD - State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China. FAU - Weng, Xiuxiu AU - Weng X AUID- ORCID: 0000-0002-4832-5643 AD - State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China. FAU - Yue, Xiangpeng AU - Yue X AD - State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China. FAU - Li, Wanhong AU - Li W AUID- ORCID: 0000-0002-2155-6863 AD - State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China. LA - eng GR - 25JRRA643,/Gansu Provincial Science and Technology Department/ GR - 25YFNH005/Gansu Provincial Science and Technology Department/ GR - CARS-38/China Agriculture Research System/ PT - Journal Article DEP - 20261006 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707486 OTO - NOTNLM OT - Schizochytrium oil OT - docosahexaenoic acid OT - rumen fermentation OT - rumen microbiota OT - serum metabolomics OT - transcriptomics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/06 CRDT- 2026/10/27 10:45 PHST- 2026/08/18 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/06 00:00 [pmc-release] AID - microorganisms14102268 [pii] AID - microorganisms-14-02268 [pii] AID - 10.3390/microorganisms14102268 [doi] PST - epublish SO - Microorganisms. 2026 Oct 6;14(10):2268. doi: 10.3390/microorganisms14102268. PMID- 42943506 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 5 TI - Differential Effects of Antibiotics on the Horizontal Transfer of blaKPC Plasmid in Clinical Klebsiella pneumoniae Strain. LID - 10.3390/microorganisms14102267 [doi] LID - 2267 AB - Horizontal transfer of blaKPC-bearing plasmids contributes significantly to the dissemination of carbapenem resistance among Enterobacterales; however, the influence of mechanistically distinct antibiotics on this process remains incompletely understood. This study investigated the effects of meropenem, gepotidacin, and omadacycline on plasmid-mediated transfer of blaKPC from the carbapenem-resistant K. pneumoniae donor strain CDC120 to the ESBL-producing K. pneumoniae recipient strain DS073. Conjugation assays were performed under sub-inhibitory antibiotic concentrations, and plasmid transfer was confirmed by PCR and antimicrobial susceptibility testing. Expression of the conjugation-associated genes traT, traS, traN, and traG was quantified by quantitative real-time PCR (qRT-PCR). Meropenem produced the highest conjugation frequency (3.07 x 10(-7) transconjugants per donor), followed by gepotidacin (4.55 x 10(-8)) and omadacycline (3.38 x 10(-9)). These differences were reflected phenotypically, with meropenem-selected transconjugants demonstrating the greatest increase in meropenem minimum inhibitory concentration (MIC), followed by gepotidacin and omadacycline. Gene expression analysis revealed antibiotic-specific regulation of conjugation-associated genes rather than uniform activation of the conjugation machinery. Among the genes examined, traS exhibited the strongest induction across all antibiotic treatments, whereas traT was consistently downregulated and traN and traG displayed treatment-dependent expression patterns. Collectively, these findings demonstrate that antibiotics differentially influence plasmid transfer efficiency and the expression of conjugation-associated genes, thereby affecting the dissemination of blaKPC-mediated carbapenem resistance. FAU - Kumkum, Sneha AU - Kumkum S AD - Titus Family Department of Clinical Pharmacy, Alfred E. Mann School of Pharmacy and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA 90033, USA. FAU - Kalu, Michelle Chioma AU - Kalu MC AUID- ORCID: 0000-0002-6990-0125 AD - Titus Family Department of Clinical Pharmacy, Alfred E. Mann School of Pharmacy and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA 90033, USA. FAU - Wong-Beringer, Annie AU - Wong-Beringer A AUID- ORCID: 0000-0003-3302-1409 AD - Titus Family Department of Clinical Pharmacy, Alfred E. Mann School of Pharmacy and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA 90033, USA. AD - Department of Pharmacy, Huntington Health, Pasadena, CA 91105, USA. LA - eng PT - Journal Article DEP - 20261005 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707849 OTO - NOTNLM OT - K. pneumoniae OT - antimicrobial resistance OT - blaKPC OT - gepotidacin OT - horizontal gene transfer OT - meropenem OT - omadacycline OT - plasmid conjugation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/05 CRDT- 2026/10/27 10:45 PHST- 2026/08/06 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/05 00:00 [pmc-release] AID - microorganisms14102267 [pii] AID - microorganisms-14-02267 [pii] AID - 10.3390/microorganisms14102267 [doi] PST - epublish SO - Microorganisms. 2026 Oct 5;14(10):2267. doi: 10.3390/microorganisms14102267. PMID- 42943504 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 5 TI - Salivary Microbiota and Network Alterations in Oral Lichen Planus with Candida Compared to Healthy Controls. LID - 10.3390/microorganisms14102265 [doi] LID - 2265 AB - Background: Oral lichen planus (OLP) is a chronic immune-mediated mucosal disorder frequently accompanied by Candida colonization. However, the polymicrobial interactions and network alterations underlying this association remain poorly understood. This study aimed to characterize bacterial-fungal community changes and interkingdom network dynamics in OLP patients with Candida detection compared to healthy controls. Methods: Unstimulated saliva samples were collected from 24 OLP patients and 31 healthy controls. Bacterial 16S rRNA (V1-V2) and fungal ITS2 regions were simultaneously sequenced using Illumina NovaSeq. Taxonomic profiling, diversity analyses, differential analysis, and intermicrobial correlations were analyzed. Results: Beta diversity analysis revealed distinct community separation between groups (p = 0.001). OLP exhibited increased Bacteroidetes and decreased Firmicutes compared to controls. Health-associated commensals (Streptococcus salivarius, Rothia mucilaginosa, Haemophilus parainfluenzae, Veillonella sp. HMT 917) were significantly depleted, while pro-inflammatory anaerobes (Prevotella, Fusobacterium, Leptotrichia, Selenomonas) were enriched. At the genus level, Candida abundance was modestly higher in the OLP group (1.38%) compared to Healthy (0.94%), and Candida albicans was the most prominent fungal species, with a mean relative abundance of 1.22% in OLP compared to 0.91% in Healthy controls, calculated using the same sample-level denominator as the genus-level values above. Network analysis demonstrated that Candida occupied a central position with expanded connectivity to disease-associated genera. The disease network showed increased negative correlations and dispersed topology, reflecting inversely correlated taxa and reduced modularity (0.206 vs. 0.260 in healthy), consistent with less distinct community compartmentalization. Conclusions: OLP is characterized by compositional dysbiosis and disrupted bacterial-fungal interaction networks. Candida may function as a network hub associated with interkingdom dysbiosis in OLP. These findings suggest that microbiome-targeted adjunctive therapies-including antifungals, selective antimicrobials, and probiotics-warrant investigation for managing Candida-associated OLP. FAU - Ju, Hye-Min AU - Ju HM AUID- ORCID: 0000-0002-9252-6717 AD - Department of Oral Medicine, Dental and Life Science Institute, School of Dentistry, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan-Si 50612, Republic of Korea. AD - Department of Oral Medicine, Dental Research Institute, School of Dentistry, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan-Si 50612, Republic of Korea. FAU - Ahn, Yong-Woo AU - Ahn YW AD - Department of Oral Medicine, Dental and Life Science Institute, School of Dentistry, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan-Si 50612, Republic of Korea. AD - Department of Oral Medicine, Dental Research Institute, School of Dentistry, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan-Si 50612, Republic of Korea. FAU - Ok, Soo-Min AU - Ok SM AUID- ORCID: 0000-0003-1776-371X AD - Department of Oral Medicine, Dental and Life Science Institute, School of Dentistry, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan-Si 50612, Republic of Korea. AD - Department of Oral Medicine, Dental Research Institute, School of Dentistry, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan-Si 50612, Republic of Korea. FAU - Jeong, Sung-Hee AU - Jeong SH AUID- ORCID: 0000-0002-6296-4775 AD - Department of Oral Medicine, Dental and Life Science Institute, School of Dentistry, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan-Si 50612, Republic of Korea. AD - Department of Oral Medicine, Dental Research Institute, School of Dentistry, Pusan National University, Busandaehak-ro 49, Mulgeum-eup, Yangsan-Si 50612, Republic of Korea. FAU - Joo, Ji-Young AU - Joo JY AUID- ORCID: 0000-0002-4050-5797 AD - Department of Periodontology, Dental Research Institute, Pusan National University Dental Hospital, Yangsan 50612, Republic of Korea. FAU - Na, Hee Sam AU - Na HS AUID- ORCID: 0000-0002-3246-4681 AD - Department of Microbiology and Immunology, Chonnam National University Medical School, Hwasun 58128, Republic of Korea. AD - Department of Oral Microbiology, School of Dentistry, Pusan National University, Yangsan 50612, Republic of Korea. LA - eng GR - HI23C0162/Ministry of Health and Welfare/ PT - Journal Article DEP - 20261005 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707814 OTO - NOTNLM OT - Candida albicans OT - interkingdom dysbiosis OT - network analysis OT - oral lichen planus OT - oral microbiota OT - salivary microbiome COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/05 CRDT- 2026/10/27 10:45 PHST- 2026/08/26 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/05 00:00 [pmc-release] AID - microorganisms14102265 [pii] AID - microorganisms-14-02265 [pii] AID - 10.3390/microorganisms14102265 [doi] PST - epublish SO - Microorganisms. 2026 Oct 5;14(10):2265. doi: 10.3390/microorganisms14102265. PMID- 42943498 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 4 TI - Transient Disturbance and Community Convergence of Coral-Associated Microbial Communities Under Acidification, Benzo[a]pyrene Pollution, and Combined Stress in Coral Acropora millepora. LID - 10.3390/microorganisms14102259 [doi] LID - 2259 AB - Ocean acidification (OA), driven by global climate change together with pollution from organic contaminants, is increasing the ecological risk faced by coral reef ecosystems. The coral holobiont comprises diverse microbial communities that collectively support the coral host in coping with and adapting to environmental stress. However, the response of the coral holobiont to the combined stress of acidification and benzo[a]pyrene (BaP) remains poorly understood. In this study, Acropora millepora was selected as a model species to investigate the responses of coral-associated microbiomes to acidification (pH 7.8), BaP exposure (10 microg.L(-1)) and their combined effects. We found that while the cell density of coral-associated Symbiodiniaceae increased significantly with prolonged exposure time in all treatment groups, and there were no temporal differences in chlorophyll-a contents. The Symbiodiniaceae community was stable within all treatments, with Cladocopium as the dominant genus, and subtype C3u had the highest relative abundance (>70%). A rapid and significant increase in rare bacteria taxa was observed in the single-stress treatments (acidification, BaP), whereas prolonged exposure led to an increasing number of unique bacteria taxa in the combined-stress group in two weeks. Surprisingly, the main community structure of core coral-associated bacteria in the combined treatment group was close to that of the control group. Across all treatments, core coral-associated bacteria (including Sphingomonadaceae and Burkholderiaceae) consistently dominated the microbial community, while core coral-associated archaea exhibited more stochastic variations. Collectively, these results highlight the critical role of coral-associated microbiomes in rapidly and flexibly responding to environmental stressors, thereby contributing to the maintenance of holobiont stability under multiple stress conditions. FAU - Qi, Zhao AU - Qi Z AUID- ORCID: 0000-0003-1760-4567 AD - College of Ecology, Hainan University, Haikou 570228, China. AD - State Key Laboratory of Marine Resources Utilization in South China Sea, Haikou 570228, China. FAU - Chen, Yumei AU - Chen Y AD - College of Ecology, Hainan University, Haikou 570228, China. AD - State Key Laboratory of Marine Resources Utilization in South China Sea, Haikou 570228, China. FAU - Yin, Lianzheng AU - Yin L AD - School of Life and Health Sciences, Hainan University, Haikou 570228, China. FAU - Jing, Hongmei AU - Jing H AUID- ORCID: 0000-0002-1795-5650 AD - State Key Laboratory of Deep-Sea Science and Intelligent Technology, Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya 572000, China. FAU - Wu, Chuanliang AU - Wu C AD - Sanya Institute of Coral Reef Ecology Research, Sanya 572000, China. FAU - Yang, Tinghan AU - Yang T AUID- ORCID: 0000-0002-3947-5527 AD - Guangxi Key Laboratory of Beibu Gulf Marine Resources, Environment and Sustainable Development, Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai 536000, China. FAU - Diao, Xiaoping AU - Diao X AD - State Key Laboratory of Marine Resources Utilization in South China Sea, Haikou 570228, China. LA - eng GR - 2021YFF0502804/Ministry of Science and Technology of the People's Republic of China/ GR - 4196070185/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261004 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707767 OTO - NOTNLM OT - Acid + BaP stress OT - acidification OT - benzo[a]pyrene OT - coral-associated microbiomes OT - core microbes COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/04 CRDT- 2026/10/27 10:45 PHST- 2026/08/03 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/04 00:00 [pmc-release] AID - microorganisms14102259 [pii] AID - microorganisms-14-02259 [pii] AID - 10.3390/microorganisms14102259 [doi] PST - epublish SO - Microorganisms. 2026 Oct 4;14(10):2259. doi: 10.3390/microorganisms14102259. PMID- 42943495 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 4 TI - Aichivirus a Infection Associated with Severe Enteric and Respiratory Disease in a Captive White Lion (Panthera leo). LID - 10.3390/microorganisms14102256 [doi] LID - 2256 AB - Kobuviruses are emerging enteric pathogens with broad host ranges, yet their pathogenic potential in wild large felids remains largely unexplored. Here, we report the first case of severe enteric and respiratory disease associated with AiV-A infection in a large felid, designated FeKoV0813, in a captive juvenile white lion (Panthera leo). The animal presented with acute respiratory distress and severe diarrhea, culminating in death despite supportive care. Comprehensive diagnostic workflows, including necropsy, histopathology, fluorescence in situ hybridization (FISH), and absolute quantitative RT-PCR, were employed to determine tissue tropism and viral dissemination. Whole-genome sequencing, phylogenetic analysis, and selection pressure assessment were conducted to characterize the viral evolutionary dynamics and host-specific constraints. The 8213-nucleotide genome exhibited typical Kobuvirus organization, clustering within the AiV-A clade alongside carnivore-derived strains from dogs, cats, and foxes. High viral RNA loads were detected primarily in the small intestine (~7.4 log(10) copies/100 ng RNA) and trachea, with dissemination to the kidney and pancreas. Histopathological analysis revealed epithelial lesions, and FISH localized viral RNA to these affected epithelial cells, while transcriptomic profiling revealed higher transcript abundance of CXCL8 and other pro-inflammatory genes in the intestine than in other tissues. Notably, selection pressure analysis indicated strong purifying selection acting on the feline VP1 gene, with no positively selected sites identified, contrasting with signatures of episodic positive selection observed in human and rodent lineages. This study provides the first report of severe respiratory and gastrointestinal disease associated with AiV-A infection in large felids, significantly expanding the known host range of kobuviruses. Our findings underscore the need for vigilant surveillance of kobuviruses in captive wildlife and highlight the potential risk of spillover infections from domestic carnivore reservoirs. FAU - Ding, Yushan AU - Ding Y AD - Guangzhou Zoo & Guangzhou Wildlife Research Center, Guangzhou 510070, China. AD - College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China. FAU - Wu, Yajiang AU - Wu Y AD - Guangzhou Zoo & Guangzhou Wildlife Research Center, Guangzhou 510070, China. FAU - Shan, Fen AU - Shan F AD - Guangzhou Zoo & Guangzhou Wildlife Research Center, Guangzhou 510070, China. FAU - Zhou, Niu AU - Zhou N AD - Guangzhou Zoo & Guangzhou Wildlife Research Center, Guangzhou 510070, China. FAU - Lv, Mengna AU - Lv M AD - Guangzhou Zoo & Guangzhou Wildlife Research Center, Guangzhou 510070, China. FAU - Wang, Chen AU - Wang C AD - Guangzhou Zoo & Guangzhou Wildlife Research Center, Guangzhou 510070, China. FAU - Liu, Minting AU - Liu M AD - Guangzhou Zoo & Guangzhou Wildlife Research Center, Guangzhou 510070, China. FAU - Li, Wanping AU - Li W AD - Guangzhou Zoo & Guangzhou Wildlife Research Center, Guangzhou 510070, China. FAU - Chen, Wu AU - Chen W AD - Guangzhou Zoo & Guangzhou Wildlife Research Center, Guangzhou 510070, China. FAU - Chen, Zujin AU - Chen Z AD - Guangzhou Zoo & Guangzhou Wildlife Research Center, Guangzhou 510070, China. LA - eng GR - GZNL2023A01001/Guangzhou Laboratory/ GR - 2026ZD01999702/Prevention and Control of Emerging and Major Infectious Diseases-National Science and Tech-nology Major Project/ GR - YL202501/Guangzhou Postdoctoral Research Funding Project/ PT - Journal Article DEP - 20261004 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707751 OTO - NOTNLM OT - Panthera leo OT - cross-species transmission OT - kobuvirus OT - transcriptomic analysis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/04 CRDT- 2026/10/27 10:45 PHST- 2026/08/17 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/04 00:00 [pmc-release] AID - microorganisms14102256 [pii] AID - microorganisms-14-02256 [pii] AID - 10.3390/microorganisms14102256 [doi] PST - epublish SO - Microorganisms. 2026 Oct 4;14(10):2256. doi: 10.3390/microorganisms14102256. PMID- 42943489 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 4 TI - Artificial Intelligence for Microbial Colony Analysis: Plate-Reading Challenges, Method Selection, and Validation. LID - 10.3390/microorganisms14102251 [doi] LID - 2251 AB - Microbial colony analysis is routinely used in clinical microbiology, pharmaceutical quality control, food safety, and environmental monitoring. Colony enumeration remains challenging because real-world plates frequently show crowding, size variation, background debris, and plate-rim interference. Low contrast on membrane or filter media, weak colony boundaries, and ultra-dense or too-numerous-to-count (TNTC) growth further complicate analysis. These conditions increase counting variability and limit rule-based counters. This review examines artificial intelligence (AI)-enabled colony analysis and links recurrent plate-reading challenges to method selection and validation requirements. We examine traditional threshold- and morphology-based pipelines, object detection, semantic segmentation, instance segmentation, and density- or keypoint-based estimation. Current evidence is stronger for plate-level triage and semi-quantitative interpretation than for direct colony enumeration. However, evidence supporting routine quantitative colony-forming unit (CFU) reporting across laboratories remains limited. Reliable deployment requires methods suited to plate format, organism, imaging conditions, and intended use, with validation in the target workflow. FAU - Li, Xue AU - Li X AD - Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China. AD - Clinical Biobank, Biomedical Engineering Facility of National Infrastructures for Translational Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College Hospital, Beijing 100730, China. FAU - Wang, Jing AU - Wang J AUID- ORCID: 0000-0002-7757-1261 AD - State Key Laboratory of Mechanical System and Vibration, School of Mechanical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China. FAU - Xiao, Meng AU - Xiao M AUID- ORCID: 0000-0003-2103-7008 AD - Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China. FAU - Jia, Peiyao AU - Jia P AD - Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China. FAU - Li, Dingding AU - Li D AD - Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China. FAU - Hu, Yu AU - Hu Y AD - Silliker Food Science Center Merieux NutriSciences (China), Merieux Nutrisciences Testing Technology (Shanghai) Co., Ltd., Shanghai 201112, China. FAU - Shi, Meng AU - Shi M AD - Yangtze River Pharmaceutical Group, Taizhou 225321, Jiangsu, China. FAU - Xu, Qiang AU - Xu Q AD - Jieyi Biotechnology (Suzhou) Co., Ltd. (Fasteur), Suzhou 215122, Jiangsu, China. FAU - Lu, Yi AU - Lu Y AD - Jieyi Biotechnology (Suzhou) Co., Ltd. (Fasteur), Suzhou 215122, Jiangsu, China. FAU - Xu, Yingchun AU - Xu Y AD - Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China. LA - eng GR - 2025-PUMCH-A-088/National High Level Hospital Clinical Research Funding/ GR - 2024ZD0532800/Noncommunicable Chronic Diseases-National Science and Technology Major Project/ PT - Journal Article PT - Review DEP - 20261004 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707560 OTO - NOTNLM OT - colony enumeration OT - digital microbiology OT - instance segmentation OT - membrane filtration OT - method validation OT - urine-culture screening COIS- Y.H. is affiliated with Merieux NutriSciences Testing Technology (Shanghai) Co., Ltd. M.S. is affiliated with Yangtze River Pharmaceutical Group. Q.X. and Y.L. are affiliated with Jieyi Biotechnology (Suzhou) Co., Ltd. (Fasteur), which develops microbiological imaging and colony-analysis systems. The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/04 CRDT- 2026/10/27 10:45 PHST- 2026/08/19 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/04 00:00 [pmc-release] AID - microorganisms14102251 [pii] AID - microorganisms-14-02251 [pii] AID - 10.3390/microorganisms14102251 [doi] PST - epublish SO - Microorganisms. 2026 Oct 4;14(10):2251. doi: 10.3390/microorganisms14102251. PMID- 42943485 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 3 TI - Antimicrobial Resistance and Genetic Characteristics of Ciprofloxacin-Resistant Escherichia coli from Suckling and Weaned Piglet Housing Areas in Korea. LID - 10.3390/microorganisms14102247 [doi] LID - 2247 AB - In swine production systems, antimicrobial exposure and management conditions vary across production stages, particularly during the transition from suckling to weaning, which may influence the selection and persistence of antimicrobial-resistant bacteria. We compared the antimicrobial resistance patterns of 70 Escherichia coli isolates obtained from suckling and weaned piglet housing areas (35 isolates each) on 35 farms in Korea and further characterized the genetic features of the 40 ciprofloxacin (CIP)-resistant isolates. Isolates showed high rates of resistance to several antimicrobials, including streptomycin (88.6%) and ampicillin (85.7%). In an exploratory comparison, CIP resistance was higher in isolates from weaned piglet housing areas (71.4%) than in those from suckling piglet housing areas (42.9%; unadjusted two-sided exact p = 0.04139, McNemar's test). Mutations in the quinolone resistance-determining regions (QRDRs) were detected in 27 of the 40 CIP-resistant isolates (67.5%), predominantly gyrA S83L/D87N and parC S80I substitutions. Plasmid-mediated quinolone resistance (PMQR) determinants were detected in nine isolates; qnrD was the most common gene (6/9, 66.7%). Multilocus sequence typing analysis of the nine PMQR-positive isolates identified multiple sequence types; ST5229 was the most common sequence type (5/9, 55.6%) and was detected in both housing-area groups. The higher CIP resistance observed in isolates from weaned piglet housing areas indicates a production-stage-associated difference in FQ resistance during early pig production. FAU - Kwon, Oyoung AU - Kwon O AD - College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea. FAU - Ryu, Da-Hye AU - Ryu DH AD - College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea. FAU - Ahn, Hyun-Jung AU - Ahn HJ AD - College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea. FAU - Choe, Su-Jin AU - Choe SJ AD - College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea. FAU - Kim, Min-Gyu AU - Kim MG AD - College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea. FAU - Go, You-Kyung AU - Go YK AD - College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea. FAU - Kim, Su-Bin AU - Kim SB AD - College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea. FAU - Kim, Soochong AU - Kim S AUID- ORCID: 0000-0002-6739-9787 AD - College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea. FAU - Lee, Seung-Hun AU - Lee SH AUID- ORCID: 0000-0002-6244-0381 AD - College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea. FAU - Do, Kyung-Hyo AU - Do KH AD - College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea. AD - Department of Companion Animal Health, Daegu Haany University, Gyeongsan 38610, Republic of Korea. FAU - Seo, Kwang-Won AU - Seo KW AUID- ORCID: 0000-0003-1620-4916 AD - College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea. LA - eng GR - RS-2026-25489744/National Research Foundation of Korea/ GR - Chungbuk National University Glocal30 project (2025)/Chungbuk National University/ PT - Journal Article DEP - 20261003 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707783 OTO - NOTNLM OT - Escherichia coli OT - fluoroquinolone resistance OT - plasmid-mediated quinolone resistance OT - quinolone resistance-determining region OT - swine production stage COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/03 CRDT- 2026/10/27 10:45 PHST- 2026/08/11 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - microorganisms14102247 [pii] AID - microorganisms-14-02247 [pii] AID - 10.3390/microorganisms14102247 [doi] PST - epublish SO - Microorganisms. 2026 Oct 3;14(10):2247. doi: 10.3390/microorganisms14102247. PMID- 42943484 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 3 TI - Sino-Cranial Infection Caused by Curvularia spicifera in a Young Adult Without Overt Immunosuppression: A Case Report and Review of the Literature. LID - 10.3390/microorganisms14102245 [doi] LID - 2245 AB - Curvularia species are melanized dematiaceous molds widely distributed in soil, plants, and decaying organic matter. Human infections are uncommon and typically involve the paranasal sinuses, skin, cornea, or respiratory tract, whereas central nervous system (CNS) involvement is rare. We report a locally invasive sino-cranial infection caused by Curvularia spicifera in a 20-year-old immunocompetent man with chronic sinusitis and features consistent with allergic fungal rhinosinusitis (AFRS). Brain magnetic resonance imaging revealed acute-on-chronic sinusitis complicated by a frontal sinus mucocele with orbital and intracranial extension. Management involved urgent neurosurgical source control followed by endoscopic sinonasal surgery. C. spicifera was repeatedly isolated from independent deep surgical specimens. The isolate was identified as Curvularia spicifera using a polyphasic approach integrating morphological, proteomic (MALDI-TOF MS), and molecular evidence. Histopathological examination demonstrated mixed neutrophilic and eosinophilic inflammation consistent with AFRS. Antifungal treatment was initiated with liposomal amphotericin B plus voriconazole; amphotericin B was subsequently discontinued because of severe hypokalemia, whereas voriconazole was continued under therapeutic drug monitoring. At the time of writing, the patient had completed three months of voriconazole therapy, with progressive clinical improvement and good tolerability, and was awaiting further radiological follow-up to guide the decision on treatment duration. Review of published cases indicates that contiguous spread from chronic sinonasal disease represents a frequent pathogenic mechanism of CNS Curvularia infection and that severe disease may occur even in the absence of overt immunosuppression. This case highlights the importance of early multidisciplinary management, aggressive surgical source control, accurate microbiological identification, and prolonged mold-active antifungal therapy with careful monitoring for treatment-related toxicity. FAU - Emiliozzi, Arianna AU - Emiliozzi A AUID- ORCID: 0000-0002-7122-7101 AD - Systemic and Immune Depression-Associated Infections Unit, National Institute for Infectious Diseases "Lazzaro Spallanzani", IRCCS, 00149 Rome, Italy. FAU - Capone, Alessandro AU - Capone A AUID- ORCID: 0000-0002-9964-1195 AD - Systemic and Immune Depression-Associated Infections Unit, National Institute for Infectious Diseases "Lazzaro Spallanzani", IRCCS, 00149 Rome, Italy. FAU - Selleri, Marina AU - Selleri M AUID- ORCID: 0000-0001-9131-7502 AD - Laboratory of Microbiology and Biobank, National Institute for Infectious Diseases "Lazzaro Spallanzani", IRCCS, 00149 Rome, Italy. FAU - Palazzo, Carlo Giacomo AU - Palazzo CG AD - Laboratory of Microbiology and Biobank, National Institute for Infectious Diseases "Lazzaro Spallanzani", IRCCS, 00149 Rome, Italy. FAU - Vulcano, Antonella AU - Vulcano A AD - Laboratory of Microbiology and Biobank, National Institute for Infectious Diseases "Lazzaro Spallanzani", IRCCS, 00149 Rome, Italy. FAU - Temperoni, Chiara AU - Temperoni C AUID- ORCID: 0009-0002-3045-7469 AD - Systemic and Immune Depression-Associated Infections Unit, National Institute for Infectious Diseases "Lazzaro Spallanzani", IRCCS, 00149 Rome, Italy. FAU - Lodi, Alessandra AU - Lodi A AD - Systemic and Immune Depression-Associated Infections Unit, National Institute for Infectious Diseases "Lazzaro Spallanzani", IRCCS, 00149 Rome, Italy. FAU - Luglietto, Davide AU - Luglietto D AUID- ORCID: 0000-0001-9744-3171 AD - Department of Neurosurgery, Azienda Ospedaliera San Camillo Forlanini, 00152 Rome, Italy. FAU - Gallo, Assunta AU - Gallo A AD - Laboratory of Microbiology, Azienda Ospedaliera San Camillo Forlanini, 00152 Rome, Italy. FAU - Ricciuti, Riccardo Antonio AU - Ricciuti RA AUID- ORCID: 0000-0003-4970-2065 AD - Department of Neurosurgery, Azienda Ospedaliera San Camillo Forlanini, 00152 Rome, Italy. FAU - Fontana, Carla AU - Fontana C AUID- ORCID: 0000-0003-2198-1947 AD - Laboratory of Microbiology and Biobank, National Institute for Infectious Diseases "Lazzaro Spallanzani", IRCCS, 00149 Rome, Italy. FAU - Cicalini, Stefania AU - Cicalini S AUID- ORCID: 0000-0002-0754-6917 AD - Systemic and Immune Depression-Associated Infections Unit, National Institute for Infectious Diseases "Lazzaro Spallanzani", IRCCS, 00149 Rome, Italy. LA - eng GR - 2801210, Ricerca Corrente, Linea 3, IRCCS INMI L. Spallanzani, 2025-2027/Ministry of Health/ PT - Case Reports PT - Journal Article DEP - 20261003 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707611 OTO - NOTNLM OT - Curvularia spicifera OT - allergic fungal rhinosinusitis OT - amphotericin B OT - cannabis OT - dematiaceous fungi OT - dog training OT - epidural empyema OT - marijuana OT - phaeohyphomycosis OT - voriconazole COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/03 CRDT- 2026/10/27 10:45 PHST- 2026/08/12 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - microorganisms14102245 [pii] AID - microorganisms-14-02245 [pii] AID - 10.3390/microorganisms14102245 [doi] PST - epublish SO - Microorganisms. 2026 Oct 3;14(10):2245. doi: 10.3390/microorganisms14102245. PMID- 42943477 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 3 TI - CpxA Deletion Is Associated with Altered Polymyxin B Susceptibility and Flagellar-Associated Transcriptional Remodeling in Salmonella Typhimurium. LID - 10.3390/microorganisms14102237 [doi] LID - 2237 AB - Polymyxin B is considered a last-resort antibiotic for multidrug-resistant or extensively drug-resistant Gram-negative bacterial infections. Understanding the determinants of polymyxin B susceptibility in Salmonella is important for public health. Phenotypic analyses showed that deletion of cpxA was associated with altered stress tolerance and a one-dilution difference in the reported polymyxin B MIC, whereas the DeltacpxADeltaarcB double mutant exhibited broader growth and stress-related defects. Benjamini-Hochberg-controlled RNA sequencing under polymyxin B exposure identified 60 differentially expressed genes (35 with higher and 25 with lower expression in DeltacpxA), including 12 flagellar-associated genes and significant enrichment of flagellar functions. RT-qPCR showed significantly higher relative expression of four selected FDR-significant flagellar-associated genes in the DeltacpxA strain; fliC, fliS, fliK, and flgE were directionally concordant with RNA-seq, and expression returned toward WT-like levels in the complemented strain. Representative micrographs illustrated differences in extracellular filamentous appearance. These findings associate cpxA deletion with selective flagellar-associated transcriptional changes under polymyxin B exposure, accompanied by a one-dilution reported MIC difference and qualitative differences in extracellular filamentous appearance. FAU - Fang, Yupeng AU - Fang Y AD - Hebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China. FAU - Guo, Kuanjia AU - Guo K AUID- ORCID: 0009-0006-8049-1189 AD - Hebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China. FAU - Zhang, Xiaoqing AU - Zhang X AD - Hebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China. FAU - Chen, Tiantian AU - Chen T AD - Hebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China. FAU - Xue, Chengbin AU - Xue C AD - Hebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China. FAU - Zhan, Yangxinyue AU - Zhan Y AD - Hebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China. FAU - Guo, Ziwei AU - Guo Z AD - Hebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China. FAU - Liu, Cunlei AU - Liu C AD - Hebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China. FAU - Lin, Hao AU - Lin H AD - Hebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China. FAU - Liu, Yu AU - Liu Y AD - Hebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China. FAU - Bayasgalan, Chimedtseren AU - Bayasgalan C AUID- ORCID: 0000-0002-7182-036X AD - School of Veterinary Medicine, Mongolian University of Life Sciences, Ulaanbaatar 17024, Mongolia. FAU - Yang, Shunli AU - Yang S AD - Hebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China. FAU - Liu, Yongsheng AU - Liu Y AD - Hebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China. LA - eng GR - 252F2901D/Hebei Provincial Department of Science and Technology/ GR - 25296601D/Hebei Provincial Department of Science and Technology/ GR - 2025YFE0118900/Ministry of Science and Technology of the People's Republic of China/ PT - Journal Article DEP - 20261003 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707502 OTO - NOTNLM OT - CpxAR two-component system OT - Salmonella Typhimurium OT - cpxA OT - envelope stress OT - flagellar assembly OT - polymyxin B susceptibility COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/03 CRDT- 2026/10/27 10:45 PHST- 2026/09/13 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - microorganisms14102237 [pii] AID - microorganisms-14-02237 [pii] AID - 10.3390/microorganisms14102237 [doi] PST - epublish SO - Microorganisms. 2026 Oct 3;14(10):2237. doi: 10.3390/microorganisms14102237. PMID- 42943474 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 3 TI - Complementary Use of Shotgun Metagenomics and Multiplex PCR for Pathogen Detection on High-Touch Surfaces. LID - 10.3390/microorganisms14102233 [doi] LID - 2233 AB - Shotgun metagenomics is being increasingly promoted as a pathogen-agnostic alternative to targeted PCR for environmental surveillance, yet its practical value on dry, low-biomass, host-dominated surfaces remains uncertain. In a companion study, we reported QIAstat-Dx multiplex PCR screening of 55 pooled high-touch surface samples from Riyadh, identifying 19 positive pools (34.5%). Here, in a retrospective exploratory comparison, we use those previously reported PCR calls as the targeted comparator and ask what unenriched shotgun metagenomic sequencing adds. Ten archived pools, each with at least one PCR-detected viral target, were sequenced (Illumina NovaSeq X Plus, 2 x 150 bp). Human-mapped reads constituted a substantial fraction of every library (mean 59.1%; range 42.1-80.2%). Among 14 evaluable PCR-positive target events, metagenomics assigned at least one read pair to the corresponding target taxon in 10 (71.4%; exact 95% CI, 41.9-91.6%). Because the cohort was preselected for viral PCR positivity, this value represents positive agreement/taxonomic concordance within the selected cohort and not diagnostic sensitivity. At the same >/=1 read-pair threshold, target taxa were also assigned to 91 of 246 (37.0%) evaluable pool-by-target cells in which PCR was negative; raising the threshold to >/=10 read pairs reduced positive agreement to 35.7% while background assignment remained 24.8%. Of six target categories with PCR-positive events, only coronavirus 229E was assigned more read pairs in its PCR-positive pool than in any PCR-negative pool. Across all ten pools, the three respiratory RNA-virus taxa represented among the PCR-positive calls accounted for only 105 classified read pairs in total-including assignments in both PCR-positive and PCR-negative pool-target cells-equivalent to 0.12% of the viral fraction. Even the broadest viral reference coverage was limited, as adenovirus in HP10 reached 16.39% (23 distinct alignment spans), while SARS-CoV-2 in CP17 reached 8.83% (12 spans); these partial, discontinuous alignments did not support robust whole-genome consensus reconstruction or genome-wide variant or phylogenetic analysis. Metagenomics nevertheless provided a broader taxonomic context beyond the targeted panel. Under this retrospective unenriched workflow, target recovery was variable, no tested assignment threshold separated a PCR-positive target signal from the background assignment, and shotgun metagenomics added ecological context rather than providing a reliable replacement for targeted multiplex PCR in this dataset. FAU - Ghiba, Mohamad Taisir Ahmad AU - Ghiba MTA AUID- ORCID: 0000-0002-1302-1682 AD - Botany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. FAU - Alsheikh-Hussain, Areej AU - Alsheikh-Hussain A AD - Computational Medicine & Bioinformatics Department, Genomic Medicine Centre of Excellence, King Faisal Specialist Hospital & Research Centre, Riyadh 12713, Saudi Arabia. FAU - Alzahrani, Turki Essa AU - Alzahrani TE AD - Computational Medicine & Bioinformatics Department, Genomic Medicine Centre of Excellence, King Faisal Specialist Hospital & Research Centre, Riyadh 12713, Saudi Arabia. FAU - Alhamdoosh, Monther AU - Alhamdoosh M AUID- ORCID: 0000-0002-2411-1325 AD - Computational Medicine & Bioinformatics Department, Genomic Medicine Centre of Excellence, King Faisal Specialist Hospital & Research Centre, Riyadh 12713, Saudi Arabia. FAU - Alhetheel, Abdulkarim Fahad AU - Alhetheel AF AUID- ORCID: 0000-0001-9257-1385 AD - Department of Pathology and Laboratory Medicine, College of Medicine, King Saud University, Riyadh 11451, Saudi Arabia. FAU - Hanif, Atif AU - Hanif A AD - Botany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. FAU - Eifan, Saleh Ahmed AU - Eifan SA AD - Botany and Microbiology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia. LA - eng GR - ORF-2026-797/King Saud University/ PT - Journal Article DEP - 20261003 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707448 OTO - NOTNLM OT - QIAstat-Dx OT - SARS-CoV-2 OT - environmental surveillance OT - fomites OT - multiplex PCR OT - shotgun metagenomics OT - viral enrichment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/03 CRDT- 2026/10/27 10:45 PHST- 2026/07/28 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - microorganisms14102233 [pii] AID - microorganisms-14-02233 [pii] AID - 10.3390/microorganisms14102233 [doi] PST - epublish SO - Microorganisms. 2026 Oct 3;14(10):2233. doi: 10.3390/microorganisms14102233. PMID- 42943463 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 2 TI - Whole-Genome Sequencing Reveals the Resistome, Virulome, Pan-Genome Architecture, and Phylogenomic Diversity of Extensively Drug-Resistant Salmonella enterica Serovar Typhi in Pakistan. LID - 10.3390/microorganisms14102225 [doi] LID - 2225 AB - The emergence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Salmonella enterica serovar Typhi has severely constrained typhoid treatment. Pakistan is a major hotspot for XDR typhoid, yet genomic evidence defining the molecular basis of antimicrobial resistance, virulence, and genomic diversity of circulating strains remains limited. A cross-sectional study was conducted from January to December 2021 involving 252 culture-confirmed S. Typhi isolates collected from patients with suspected typhoid fever in Peshawar, Pakistan. Isolates were identified by conventional microbiological methods, biochemical testing, and 16S rRNA PCR. Antimicrobial susceptibility testing (AST) was performed according to CLSI guidelines, and isolates were classified as susceptible, MDR, or XDR following WHO criteria. Fourteen non-duplicate XDR isolates were selected for Illumina whole-genome sequencing (WGS). Bioinformatic analyses included genome assembly, multilocus sequence typing (MLST), resistome and virulome characterization, mutation analysis, pan-genome analysis, and core-genome phylogenetic reconstruction. Pearson's chi-square goodness-of-fit test was used to assess categorical distributions, with p < 0.05 considered statistically significant. Among the 252 isolates, 152 (60.3%) were XDR, 73 (29.0%) were MDR, and 27 (10.7%) were susceptible. The distributions of sex, age group, and phenotypic classification differed significantly from equal expected proportions (p = 0.001, p = 0.013, and p < 0.001, respectively). Resistance was highest to ciprofloxacin, ampicillin, chloramphenicol, ceftriaxone, and co-Trimoxazole, whereas azithromycin and meropenem showed high in vitro activity. WGS showed that all sequenced isolates belonged to sequence type ST1 and had conserved genome sizes (4.84-4.95 Mb) with ~54% GC content. The resistome included blaCTX-M-15, blaTEM-1, catA1, sul1, qnrS1, aac(6')-Iy, and dfrA7, with mutations in gyrA, parC, soxS, pmrA, and pmrB. Virulence genes associated with adhesion, type III secretion, Vi capsule biosynthesis, intracellular survival, iron acquisition, and typhoid toxin were conserved across all isolates. Pan-genome analysis indicated limited pan-genome expansion within the sequenced collection, while phylogenomic analysis showed high genetic relatedness among the sequenced isolates, indicating substantial genomic conservation within this collection. The high genomic relatedness and limited gene-content variation observed within the 14 sequenced XDR isolates support a genetically conserved collection, but do not by themselves establish ongoing transmission or clonal expansion. Larger and more geographically diverse genomic datasets are needed to determine the broader population structure of XDR S. Typhi in Pakistan. Continued genomic surveillance, appropriate antibiotic use, typhoid conjugate vaccination, and effective infection-control measures are needed to reduce transmission of drug-resistant typhoid. FAU - Mehr, Rabia AU - Mehr R AD - Department of Zoology, Islamia College Peshawar, Peshawar 25120, Pakistan. FAU - Ali, Qaisar AU - Ali Q AUID- ORCID: 0000-0003-1556-4987 AD - Center of biotechnology and microbiology, University of Peshawar, Peshawar 25120, Pakistan. FAU - Zahid, Muhammad AU - Zahid M AD - Department of Zoology, Islamia College Peshawar, Peshawar 25120, Pakistan. FAU - Khan, Taj Ali AU - Khan TA AD - Institute of Pathology and Diagnostic Medicine, Khyber Medical University, Peshawar 25100, Pakistan. AD - Public Health Reference Laboratory (PHRL), Khyber Medical University (KMU), Phase 5, Hayatabad, Peshawar 25000, Pakistan. FAU - Asghar, Muhammad AU - Asghar M AD - Institute of Pathology and Diagnostic Medicine, Khyber Medical University, Peshawar 25100, Pakistan. AD - Department of Pathology, Khyber Teaching Hospital, Khyber Medical College, Peshawar 25120, Pakistan. FAU - Rehman, Noor AU - Rehman N AD - Department of Pathology, Khyber Teaching Hospital, Khyber Medical College, Peshawar 25120, Pakistan. FAU - Clark, Taane G AU - Clark TG AUID- ORCID: 0000-0001-8985-9265 AD - Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK. AD - Faculty of Epidemiology and Population Health, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK. FAU - Uddin, Reaz AU - Uddin R AUID- ORCID: 0000-0002-9928-4482 AD - Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan. FAU - Khan, Ajmal AU - Khan A AD - Natural and Medical Sciences Research Center, University of Nizwa, PO Box 33, Nizwa 616, Oman. AD - Department of Chemical and Biological Engineering, College of Engineering, Korea University, Seoul 02841, Republic of Korea. LA - eng GR - MRC MR/X005895/1; BBSRC BB/X018156/1; EPSRC EP/Y018842/1/UK Research and Innovation/ GR - Grant No. 99436/HEC pakistan/ PT - Journal Article DEP - 20261002 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707774 OTO - NOTNLM OT - Salmonella enterica serovar Typhi OT - antimicrobial resistance OT - extensively drug-resistant OT - multidrug resistance OT - phylogenomics OT - resistome OT - virulome OT - whole-genome sequencing COIS- The authors report no competing interests. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/02 CRDT- 2026/10/27 10:45 PHST- 2026/08/19 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/02 00:00 [pmc-release] AID - microorganisms14102225 [pii] AID - microorganisms-14-02225 [pii] AID - 10.3390/microorganisms14102225 [doi] PST - epublish SO - Microorganisms. 2026 Oct 2;14(10):2225. doi: 10.3390/microorganisms14102225. PMID- 42943461 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 2 TI - Effects of Protein Levels of Diets and Yeast Culture Supplementation on Performance, Antioxidant Capacity, and Intestinal Health in Laying Hens. LID - 10.3390/microorganisms14102223 [doi] LID - 2223 AB - Yeast culture (YC) can improve laying hen performance, but whether this effect depends on dietary protein level remains unclear. This experiment investigated the effects of adding YC to diets with different protein levels on the performance and intestinal health of laying hens. A total of 504 Lohmann pink laying hens aged 55 weeks were selected and randomly assigned to 4 treatments, with 7 replicates per treatment and 18 hens per replicate. A 2 x 2 factorial design was used, with two CP levels of 15.5% (NP) and 14.5% (LCP), and two YC supplementation levels of 0% and 1%. The feeding trial lasted 12 weeks. YC significantly increased the laying rate (p < 0.05). CP and YC interacted to affect the laying rate (p < 0.05). YC significantly increased serum CAT and GSH-Px activities, reduced PCO, and increased trypsin activity (p < 0.05). CP and YC interacted to affect GSH-Px and trypsin activities (p < 0.05). GSH-Px and trypsin activities were significantly higher in the LCP-YC group than in the LCP-0 group (p < 0.05). YC significantly increased jejunal villus height (VH) (p < 0.05). A significant CP x YC interaction was observed for jejunal VH; supplementing the LCP diet with YC significantly increased VH (p < 0.05). YC significantly increased the percentage of goblet cells in the jejunum, the contents of IgG and IgM in the jejunal mucosa, and decreased TNF-alpha content (p < 0.05). IFN-gamma content was also significantly lower in the LCP-YC group than in the LCP-0 group (p < 0.05). YC affected cecal microbial alpha-diversity, while CP and YC interacted to affect beta-diversity and the relative abundance of certain bacterial taxa (p < 0.05). In conclusion, under these experimental conditions, YC improves the laying rate and antioxidant capacity and supports intestinal health in laying hens. A CP x YC interaction was observed, but the apparently stronger response to YC in the LCP group should be interpreted cautiously because the LCP-0 and LCP-YC diets differed in analyzed calcium concentration. FAU - Chen, Siyu AU - Chen S AUID- ORCID: 0009-0006-1284-5168 AD - Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China. FAU - Zhang, Keying AU - Zhang K AUID- ORCID: 0000-0002-2909-3357 AD - Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China. FAU - Wang, Jianping AU - Wang J AUID- ORCID: 0000-0002-6596-8771 AD - Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China. FAU - Bai, Shiping AU - Bai S AUID- ORCID: 0000-0002-5775-1900 AD - Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China. FAU - Zeng, Qiufeng AU - Zeng Q AD - Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China. FAU - Wang, Wenpan AU - Wang W AD - Shaanxi Xinhanbao Biotechnology Co., Ltd., Xianyang 712000, China. FAU - Wang, Liang AU - Wang L AD - Shaanxi Xinhanbao Biotechnology Co., Ltd., Xianyang 712000, China. FAU - Yang, Penghui AU - Yang P AD - Shaanxi Xinhanbao Biotechnology Co., Ltd., Xianyang 712000, China. FAU - Xuan, Yue AU - Xuan Y AD - Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China. FAU - Ding, Xuemei AU - Ding X AD - Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China. LA - eng GR - SCCXTD-2026-24/the Innovation Team Program of Sichuan/ PT - Journal Article DEP - 20261002 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707503 OTO - NOTNLM OT - antioxidant capacity OT - intestinal health OT - laying hens OT - production performance OT - yeast culture COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/02 CRDT- 2026/10/27 10:45 PHST- 2026/08/15 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/02 00:00 [pmc-release] AID - microorganisms14102223 [pii] AID - microorganisms-14-02223 [pii] AID - 10.3390/microorganisms14102223 [doi] PST - epublish SO - Microorganisms. 2026 Oct 2;14(10):2223. doi: 10.3390/microorganisms14102223. PMID- 42943453 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 2 TI - Syndromic Testing in Clinical Microbiology and Infectious Diseases: An Updated Review of Diagnostic Performance, Clinical and Healthcare Impact, and Emerging Developments. LID - 10.3390/microorganisms14102214 [doi] LID - 2214 AB - Syndromic testing, defined as the simultaneous nucleic acid amplification-based detection of multiple potential pathogens and antimicrobial resistance (AMR) determinants directly from clinical specimens, has transformed laboratory diagnosis of infectious diseases in the United States over the past two decades. In contrast to traditional microbiology workflows that rely on culture, phenotypic susceptibility testing, and sequential single-target assays with turnaround times of 24-72 h or longer, syndromic multiplex panels provide comprehensive pathogen identification within 1-5 h, enabling timely clinical decision-making, targeted antimicrobial therapy, and improved patient outcomes. In this review, the current landscape of FDA-cleared syndromic testing panels across seven major clinical syndromes is examined, including respiratory tract infections, pneumonia, bloodstream infections, gastrointestinal infections, meningitis/encephalitis, joint and bone infections, and global febrile/travel-related illnesses. For each syndrome, commercially available panels in the United States are summarized, with emphasis on diagnostic performance, clinical applications, and limitations. Available evidence demonstrates that syndromic testing, when integrated with institutional diagnostic and antimicrobial stewardship programs, can reduce time to optimal therapy and unnecessary antimicrobial use and, in selected settings, shorten hospital length of stay. A mortality benefit has been reported in some patient populations, particularly those with bloodstream infections. Nevertheless, rapid turnaround time and earlier pathogen identification do not necessarily translate into timely clinical action and improved patient outcomes. Challenges related to implementation and optimization of appropriate panel utilization, including evolving AMR epidemiology, dynamic and geographically variable pathogen distributions, the critical role of diagnostic and antimicrobial stewardship, and emerging developments for diagnosis and management of infectious disease, are also discussed. FAU - Wang, Guiqing AU - Wang G AUID- ORCID: 0000-0001-6590-5394 AD - Department of Pathology, New York University Grossman School of Medicine and NYU Langone Health Medical Center, New York, NY 10016, USA. FAU - Chen, Fei AU - Chen F AUID- ORCID: 0000-0001-8005-4399 AD - Department of Pathology, New York University Grossman School of Medicine and NYU Langone Health Medical Center, New York, NY 10016, USA. LA - eng PT - Journal Article PT - Review DEP - 20261002 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707882 OTO - NOTNLM OT - NAAT OT - antimicrobial resistance OT - antimicrobial stewardship OT - bloodstream infection OT - diagnostic stewardship OT - gastrointestinal panel OT - joint infection OT - meningitis/encephalitis panel OT - multiplex PCR OT - pneumonia OT - respiratory panel OT - syndromic testing OT - tropical fever COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/02 CRDT- 2026/10/27 10:45 PHST- 2026/08/02 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/02 00:00 [pmc-release] AID - microorganisms14102214 [pii] AID - microorganisms-14-02214 [pii] AID - 10.3390/microorganisms14102214 [doi] PST - epublish SO - Microorganisms. 2026 Oct 2;14(10):2214. doi: 10.3390/microorganisms14102214. PMID- 42943448 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 1 TI - Synthetic Biology and Systems Metabolic Engineering of Aminoglycoside Antibiotics: From Biosynthetic Pathways to Resistance-Evading and Low-Toxicity Design. LID - 10.3390/microorganisms14102209 [doi] LID - 2209 AB - Aminoglycoside antibiotics are important therapeutic agents in the clinical treatment of Gram-negative bacterial infections; however, the spread of antimicrobial resistance, together with dose-limiting ototoxicity and nephrotoxicity side effects, substantially restricts their clinical utility. Recent advances in synthetic biology and systems metabolic engineering have provided powerful approaches for elucidating and engineering the biosynthetic pathways of these antibiotics. This review systematically summarizes the conserved biosynthetic logic of aminoglycosides, with particular emphasis on the 2-deoxystreptamine and streptidine core scaffold assembly and on the catalytic features and substrate selectivity of four major enzyme families involved in aminoglycoside biosynthesis: glycosyltransferases, pyridoxal phosphate-dependent aminotransferases, S-adenosyl-L-methionine-dependent methyltransferases, and phosphorylation/deamination enzymes. On this basis, the core strategies for synthetic biology and systems metabolic engineering of aminoglycoside antibiotics are reviewed from five perspectives: genetic circuit design, chassis cell optimization, cell-free synthesis, heterologous de novo biosynthesis, and systems biology-driven global optimization. Using gentamicin, kanamycin, tobramycin, apramycin, neomycin, and streptomycin as representative examples, we highlight the distinctive features of their respective biosynthetic pathways and discuss their potential for synthetic biology-based exploitation. Particular attention is given to the development of novel derivatives, including resistance-evading derivatives such as plazomicin, low-toxicity designs based on 4-monosubstituted apramycin structures, and advances in combinatorial biosynthesis for generating hybrid antibiotics. Finally, key challenges are examined, including heterologous expression compatibility, bottlenecks in product yield, and an incomplete understanding of structure-activity relationships. Emerging strategies for overcoming these challenges are also discussed, with a focus on cutting-edge technologies such as artificial intelligence-driven dynamic regulation, genome-scale metabolic model-guided design, and cell-free synthesis systems. FAU - Chu, Shan AU - Chu S AD - College of Medical Engineering, Jining Medical University, Jining 272067, China. FAU - Chu, Yuqing AU - Chu Y AD - College of Medical Engineering, Jining Medical University, Jining 272067, China. FAU - Huang, Mohan AU - Huang M AD - College of Medical Engineering, Jining Medical University, Jining 272067, China. FAU - Lu, Haoran AU - Lu H AD - College of Medical Engineering, Jining Medical University, Jining 272067, China. FAU - Liu, Zhiqiao AU - Liu Z AD - College of Medical Engineering, Jining Medical University, Jining 272067, China. FAU - Wang, Shuo AU - Wang S AD - College of Medical Engineering, Jining Medical University, Jining 272067, China. FAU - Zhao, Hongquan AU - Zhao H AD - College of Medical Engineering, Jining Medical University, Jining 272067, China. FAU - Chen, Xiaotang AU - Chen X AUID- ORCID: 0000-0003-0467-1488 AD - College of Medical Engineering, Jining Medical University, Jining 272067, China. LA - eng GR - ZR2023QH150/Natural Science Foundation of Shandong Province/ GR - 202113050634/Projects of medical and health technology development plan in Shandong Province/ PT - Journal Article PT - Review DEP - 20261001 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707489 OTO - NOTNLM OT - aminoglycoside antibiotics OT - antimicrobial resistance OT - biosynthetic pathways OT - synthetic biology engineering OT - systems metabolic engineering OT - toxicity optimization COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:45 PHST- 2026/09/09 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - microorganisms14102209 [pii] AID - microorganisms-14-02209 [pii] AID - 10.3390/microorganisms14102209 [doi] PST - epublish SO - Microorganisms. 2026 Oct 1;14(10):2209. doi: 10.3390/microorganisms14102209. PMID- 42943443 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 1 TI - SaCas9-Mediated CrtYB Disruption Coupled with Heterologous alpha-Bisabolol Production in Rhodotorula mucilaginosa JY1105. LID - 10.3390/microorganisms14102205 [doi] LID - 2205 AB - Rhodotorula mucilaginosa is a promising host for terpene biosynthesis, but its rational engineering remains constrained by limited genome-editing tools. Here, we developed a Staphylococcus aureus Cas9 (SaCas9) workflow in R. mucilaginosa JY1105 for CrtYB disruption and demonstrated heterologous alpha-bisabolol production. Five sgRNAs targeted CrtYB, encoding bifunctional phytoene synthase/lycopene cyclase; its disruption provided visible depigmentation for preliminary screening. Among 25 sequenced transformants per guide, CrtYB3 and CrtYB5 yielded 10 (40%) and 4 (16%) white colonies, respectively; each white colony carried an on-target deletion of 11-26 bp, whereas all red colonies retained the wild-type target sequence. No white colonies or target-site deletions were detected for CrtYB1, CrtYB2, or CrtYB4. A codon-optimized chamomile TPS1 cassette encoding alpha-bisabolol synthase was integrated by random integration into wild-type JY1105 and the edited R1-DeltaCrtYB derivative. The selected highest producers produced 37.52 +/- 3.09 and 41.45 +/- 2.08 mg L(-1) alpha-bisabolol, respectively. To our knowledge, this is the first report of heterologous alpha-bisabolol production in R. mucilaginosa. This study demonstrates the potential of R. mucilaginosa as an editable chassis for heterologous sesquiterpene biosynthesis and provides a foundation for further development of SaCas9-assisted metabolic engineering in this non-conventional yeast. FAU - Sun, Wenyi AU - Sun W AD - School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China. FAU - Liu, Haoyang AU - Liu H AD - School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China. AD - State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. FAU - Dong, Yaqi AU - Dong Y AD - State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. FAU - Li, Haoyu AU - Li H AD - State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. FAU - Wang, Yanan AU - Wang Y AD - State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China. FAU - Cai, Qian AU - Cai Q AUID- ORCID: 0000-0001-6746-5513 AD - School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China. LA - eng PT - Journal Article DEP - 20261001 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707722 OTO - NOTNLM OT - CRISPR/Cas9 OT - CrtYB OT - Rhodotorula mucilaginosa OT - SaCas9 OT - terpene biosynthesis OT - alpha-bisabolol COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:45 PHST- 2026/08/29 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - microorganisms14102205 [pii] AID - microorganisms-14-02205 [pii] AID - 10.3390/microorganisms14102205 [doi] PST - epublish SO - Microorganisms. 2026 Oct 1;14(10):2205. doi: 10.3390/microorganisms14102205. PMID- 42943439 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 1 TI - Biocidal Activity of Selected Essential Oils Against Bacterial and Fungal Pathogens and Their Cytotoxicity Toward Human Cell Lines. LID - 10.3390/microorganisms14102196 [doi] LID - 2196 AB - The growing global burden of antimicrobial resistance (AMR) highlights the urgency of identifying new therapeutic options or adjuvant agents capable of limiting microbial growth. This study aimed to evaluate the potential applicability of selected essential oils (EOs) as alternative antimicrobial agents by assessing their biocidal activity against clinically relevant microorganisms and determining their cytotoxicity toward human cell lines. Serial dilutions of the studied EOs were prepared to determine the minimum bactericidal (MBC), minimum fungicidal (MFC), and minimum biofilm eradication concentrations (MBEC) against Staphylococcus aureus ATCC((R)) 25923, Escherichia coli ATCC((R)) 25922, Candida albicans ATCC((R)) 90028, and Nakaseomyces glabratus ATCC((R)) 15545. Cytotoxicity towards cell lines was evaluated using the MTT assay. The tested EOs displayed significant biocidal effects against studied microorganisms in both planktonic and biofilm forms. Cinnamon bark oil and cinnamaldehyde showed the highest biocidal activity against S. aureus ATCC((R)) 25923, E. coli ATCC((R)) 25922, C. albicans ATCC((R)) 90028, and N. glabratus ATCC((R)) 15545, except for S. aureus in biofilm form. For thymol, yeast strains exhibited eight-fold lower susceptibility compared to bacterial strains. Cytotoxicity of the tested EOs towards the studied cell lines was significant. EOs proved to be effective against microorganisms but at the same time showed substantial cytotoxicity, thus potentially limiting their application. Further research on formulation strategies, selective delivery, and dose optimization is required to enhance their safety profile and clinical feasibility. FAU - Wozniak, Antoni AU - Wozniak A AUID- ORCID: 0009-0007-1955-1063 AD - Department of Molecular Medical Microbiology, Faculty of Medicine, Medical College, Jagiellonian University, Czysta 18, 31-008 Krakow, Poland. FAU - Drozdz, Kamil AU - Drozdz K AD - Department of Molecular Medical Microbiology, Faculty of Medicine, Medical College, Jagiellonian University, Czysta 18, 31-008 Krakow, Poland. FAU - Biegun-Drozdz, Katarzyna AU - Biegun-Drozdz K AD - Department of Molecular Medical Microbiology, Faculty of Medicine, Medical College, Jagiellonian University, Czysta 18, 31-008 Krakow, Poland. FAU - Gosiewski, Tomasz AU - Gosiewski T AUID- ORCID: 0000-0003-4725-5943 AD - Department of Molecular Medical Microbiology, Faculty of Medicine, Medical College, Jagiellonian University, Czysta 18, 31-008 Krakow, Poland. FAU - Cudak, Tomasz AU - Cudak T AUID- ORCID: 0009-0001-1039-0693 AD - Faculty of Metals Engineering and Industrial Computer Science, AGH University of Krakow, Av. A. Mickie-wicza 30, 30-059 Krakow, Poland. FAU - Karpinski, Robert AU - Karpinski R AD - Faculty of Metals Engineering and Industrial Computer Science, AGH University of Krakow, Av. A. Mickie-wicza 30, 30-059 Krakow, Poland. FAU - Fiolek, Aleksandra AU - Fiolek A AUID- ORCID: 0000-0001-5977-1481 AD - Faculty of Metals Engineering and Industrial Computer Science, AGH University of Krakow, Av. A. Mickie-wicza 30, 30-059 Krakow, Poland. FAU - Bloniarz, Aleksandra AU - Bloniarz A AUID- ORCID: 0000-0002-5358-0462 AD - Faculty of Metals Engineering and Industrial Computer Science, AGH University of Krakow, Av. A. Mickie-wicza 30, 30-059 Krakow, Poland. FAU - Marchewka, Jakub AU - Marchewka J AUID- ORCID: 0000-0002-3475-5984 AD - Faculty of Materials Science and Ceramics, AGH University of Krakow, Av. A. Mickiewicza 30, 30-059 Krakow, Poland. FAU - Moskalewicz, Tomasz AU - Moskalewicz T AUID- ORCID: 0000-0001-7142-452X AD - Faculty of Metals Engineering and Industrial Computer Science, AGH University of Krakow, Av. A. Mickie-wicza 30, 30-059 Krakow, Poland. FAU - Brzychczy-Wloch, Monika AU - Brzychczy-Wloch M AD - Department of Molecular Medical Microbiology, Faculty of Medicine, Medical College, Jagiellonian University, Czysta 18, 31-008 Krakow, Poland. LA - eng GR - 2022/45/B/ST5/00242/National Science Centre/ PT - Journal Article DEP - 20261001 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707768 OTO - NOTNLM OT - MTT assay OT - antimicrobial resistance OT - biocidal activity OT - cytotoxicity OT - essential oils OT - minimum bactericidal concentration COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:45 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - microorganisms14102196 [pii] AID - microorganisms-14-02196 [pii] AID - 10.3390/microorganisms14102196 [doi] PST - epublish SO - Microorganisms. 2026 Oct 1;14(10):2196. doi: 10.3390/microorganisms14102196. PMID- 42943437 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 1 TI - Isolation and Genome-Resolved Characterisation of Selected Indigenous Lactic Acid Bacteria from Traditional Fermented Dairy Products of Kazakhstan. LID - 10.3390/microorganisms14102201 [doi] LID - 2201 AB - Traditional fermented dairy products of Kazakhstan represent potential sources of indigenous lactic acid bacteria (LAB). However, the cultivable LAB associated with these products remain insufficiently characterised. This study aimed to isolate and taxonomically characterise cultivable LAB recovered from traditional dairy products of Kazakhstan using a culture-dependent workflow combined with MALDI-TOF MS, 16S rRNA gene sequencing, phylogenetic analysis, and whole-genome sequencing (WGS) of selected isolates. A total of 102 samples, including kumis, shubat, raw mare's milk, raw camel's milk, and freshly prepared soft camel milk cheese, were analysed. Samples were cultured on MRS agar under aerobic conditions at 30 degrees C for 48 h, and colonies were collected from randomly selected sectors of the plates and purified for further analysis, irrespective of their morphology. In total, 315 bacterial isolates were recovered, of which 79 were confirmed as LAB and retained for further taxonomic analysis. Eight LAB species belonging to four genera were identified, with Leuconostoc mesenteroides being predominant among the recovered LAB isolates (48/79, 60.8%), followed by Lactococcus lactis (19/79, 24.1%). Lentilactobacillus hilgardii, Lentilactobacillus kefiri, and Lentilactobacillus diolivorans were detected only among isolates recovered from fermented products, whereas Lacticaseibacillus paracasei was detected at low frequency. Five representative isolates, including both frequently and less frequently recovered species, were selected for WGS. WGS supported the taxonomic assignments and yielded draft genome assemblies ranging from 1.68 to 2.69 Mb, with GC contents of 34.8-43.1%. OrthoANI analysis further supported their species-level assignments and enabled subspecies-level resolution for selected isolates. Overall, the study establishes a culture-based collection of indigenous LAB recovered from traditional dairy products of Kazakhstan, representing the genera Leuconostoc, Lactococcus, Lentilactobacillus, and Lacticaseibacillus. The observed LAB species composition reflects the cultivable fraction recovered under the applied cultivation and colony-selection conditions and should not be interpreted as a comprehensive representation of LAB diversity in the investigated products. The resulting strain collection and genome sequences provide a basis for subsequent comparative genomic, physiological, and technological studies of selected indigenous LAB. FAU - Utebaeva, Aidana AU - Utebaeva A AUID- ORCID: 0000-0003-3792-4656 AD - Textile and Food Engineering Higher School, Mukhtar Auezov South-Kazakhstan University, Tauke Khan Avenue 5, Shymkent 160012, Kazakhstan. FAU - Gabrilyants, Eleonora AU - Gabrilyants E AD - Textile and Food Engineering Higher School, Mukhtar Auezov South-Kazakhstan University, Tauke Khan Avenue 5, Shymkent 160012, Kazakhstan. FAU - Abish, Zhansaya AU - Abish Z AUID- ORCID: 0000-0001-7175-9354 AD - Textile and Food Engineering Higher School, Mukhtar Auezov South-Kazakhstan University, Tauke Khan Avenue 5, Shymkent 160012, Kazakhstan. FAU - Ablash, Aigerim AU - Ablash A AD - Textile and Food Engineering Higher School, Mukhtar Auezov South-Kazakhstan University, Tauke Khan Avenue 5, Shymkent 160012, Kazakhstan. FAU - Ciprovica, Inga AU - Ciprovica I AD - Food Institute, Faculty of Agriculture and Food Technology, Latvia University of Life Sciences and Technologies, LV-3001 Jelgava, Latvia. LA - eng GR - AP26199549 "Development of highly effective associations of starter cultures of domestic origin to improve the competitiveness of dairy products in Kazakhstan"/Ministry of Science and Higher Education of the Republic of Kazakhstan/ PT - Journal Article DEP - 20261001 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707972 OTO - NOTNLM OT - Lactococcus lactis OT - Lentilactobacillus ssp. OT - Leuconostoc mesenteroides OT - indigenous strains OT - lactic acid bacteria OT - traditional dairy products OT - whole-genome sequencing COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:45 PHST- 2026/08/12 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - microorganisms14102201 [pii] AID - microorganisms-14-02201 [pii] AID - 10.3390/microorganisms14102201 [doi] PST - epublish SO - Microorganisms. 2026 Oct 1;14(10):2201. doi: 10.3390/microorganisms14102201. PMID- 42943436 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 1 TI - Genetic Proxies for Helicobacter pylori Serology Are Dominated by HLA Class II Haplotype Structure: Implications for Reported Associations with Neurodegenerative Disease. LID - 10.3390/microorganisms14102193 [doi] LID - 2193 AB - Genetic variants that predict anti-Helicobacter pylori immunoglobulin G (IgG) seropositivity lie almost entirely within the major histocompatibility complex (MHC), which also carries established risk loci for several diseases. Mendelian randomisation studies using such variants have reported causal effects of H. pylori infection on neurodegenerative disease, although the phenotype indexed is anti-H. pylori IgG seropositivity, not infection itself. We evaluated 22 candidate variants from a Japanese genome-wide study of anti-H. pylori IgG antibody titre in two-sample Mendelian randomisation analyses across five prespecified genomic proxy sets; the number retained varied by outcome. Higher genetically predicted anti-H. pylori IgG seropositivity was associated with lower Alzheimer's disease risk (odds ratio 0.83, 95% confidence interval 0.73 to 0.95). The association was absent once MHC variants were removed (0.94, 0.78 to 1.12), was strongest using HLA class II variants alone (0.73, 0.59 to 0.91), and traced to two variants near HLA-DRB1 and HLA-DQA1. Those proxies did not predict peptic ulcer disease in an ancestry-matched Japanese study (1.02, 0.92 to 1.13), differing from Parkinson's disease (0.73, 0.55 to 0.97; difference p = 0.029). Colocalisation did not support a shared causal variant for the serological and disease signals, selected proxy associations were attenuated after conditioning on leading class II variants, and the haplotypes tagged by the proxies differed between European and East Asian reference panels, indicating limited cross-ancestry transferability. The behaviour of these proxies is dominated by HLA class II haplotype structure, and they are therefore unsuitable for inferring a protective effect of H. pylori infection on neurodegenerative disease from IgG seropositivity. FAU - Lee, Hyuk AU - Lee H AUID- ORCID: 0000-0003-4271-7205 AD - Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Republic of Korea. AD - Samsung Precision Genome Medicine Institute, Samsung Medical Center, Seoul 06351, Republic of Korea. FAU - Min, Yang Won AU - Min YW AD - Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Republic of Korea. AD - Samsung Precision Genome Medicine Institute, Samsung Medical Center, Seoul 06351, Republic of Korea. FAU - Kim, Tae Jun AU - Kim TJ AD - Division of Gastroenterology, Department of Internal Medicine, Hallym University Dongtan Sacred Heart Hospital, Hallym University College of Medicine, Hwaseong 18450, Republic of Korea. LA - eng GR - RS-2024-00437038/Korea Health Industry Development Institute/ PT - Journal Article DEP - 20261001 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707645 OTO - NOTNLM OT - HLA class II OT - Helicobacter pylori OT - Mendelian randomisation OT - instrumental variables OT - linkage disequilibrium OT - major histocompatibility complex OT - neurodegenerative disease OT - serology COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:45 PHST- 2026/08/14 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - microorganisms14102193 [pii] AID - microorganisms-14-02193 [pii] AID - 10.3390/microorganisms14102193 [doi] PST - epublish SO - Microorganisms. 2026 Oct 1;14(10):2193. doi: 10.3390/microorganisms14102193. PMID- 42943431 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Oct 1 TI - Bioprocess Intensification Using Perfusion Culture for Recombinant Protein Production from Lactococcus lactis. LID - 10.3390/microorganisms14102191 [doi] LID - 2191 AB - Secretion of recombinant proteins from microbial cell factories is a promising strategy for recombinant protein biomanufacturing as it simplifies downstream processing. In this study, we sought to intensify the Lactococcus lactis high-cell-density culture, using basic fibroblast growth factor 2 (FGF2) as a model recombinant protein. We implemented a perfusion strategy using tangential flow filtration for cell retention, allowing the continuous removal of inhibitory metabolites while replenishing fresh nutrients. When conventional 2 x GM17 medium was used, the approach outperformed batch cultivation, achieving a 4.7-fold increase in biomass and a 3-fold increase in secreted FGF2, reaching a titer of 10,416 +/- 892 microg.L(-1). Concurrently, we developed a bioprocess model for L. lactis grown in a fortified spent cell culture medium, enabling systematic exploration of operating conditions. A Pareto front was generated for FGF2 titer against media usage, and perfusion profiles balancing both competing objectives were identified. An experimentally selected operating point validated the model predictions, yielding a final OD(600) of 49.2 +/- 0.3 and FGF2 titer of 2166 +/- 161 microg.L(-1) FGF2, which were 7.9-fold and 5-fold higher than batch process respectively. Overall, this work demonstrates a perfusion-based intensification strategy for L. lactis and highlights the utility of model-guided process decision-making to enhance productivity while reducing waste. FAU - Mainali, Prashant AU - Mainali P AD - Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01 Centros, Singapore 138668, Singapore. FAU - Tan, Jin Hao AU - Tan JH AUID- ORCID: 0009-0004-3640-934X AD - Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01 Centros, Singapore 138668, Singapore. FAU - Ho, Pooi Leng AU - Ho PL AD - Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01 Centros, Singapore 138668, Singapore. FAU - Chua, Melvin AU - Chua M AD - Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01 Centros, Singapore 138668, Singapore. FAU - Chua, Jiaxin AU - Chua J AD - Department of Food Science & Technology, National University of Singapore, 2 Science Drive 2, Block S14, #06, Singapore 117543, Singapore. FAU - Ow, Dave Siak-Wei AU - Ow DS AUID- ORCID: 0000-0002-0436-1896 AD - Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01 Centros, Singapore 138668, Singapore. LA - eng GR - W23W2D0009/Singapore Food Agency/ GR - M24N8C0107/Agency for Science, Technology and Research/ PT - Journal Article DEP - 20261001 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707899 OTO - NOTNLM OT - Lactococcus lactis OT - basic fibroblast growth factor (FGF2) OT - continuous biomanufacturing OT - high-cell-density culture OT - pareto front OT - perfusion COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:45 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - microorganisms14102191 [pii] AID - microorganisms-14-02191 [pii] AID - 10.3390/microorganisms14102191 [doi] PST - epublish SO - Microorganisms. 2026 Oct 1;14(10):2191. doi: 10.3390/microorganisms14102191. PMID- 42943409 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Sep 25 TI - Generational Succession of Rectal Microbiome and Metabolome in Holstein-Norwegian Red Crossbred Offspring and Their Regulatory Effects on Milk Quality. LID - 10.3390/microorganisms14102169 [doi] LID - 2169 AB - This study aimed to explore the generational succession rules of rectal microbes and metabolites in crossbred dairy cows, and clarify the regulatory mechanism underlying generational differentiation of milk quality traits, so as to provide a theoretical basis for improving milk quality and realizing precision breeding of dairy cows based on hindgut microecology. In this experiment, 10 healthy cows in mid-lactation were selected from each of three consecutive generations (F(1), F(2), and F(3)) of Holstein-Norwegian Red crossbred cattle, totaling 30 cows. High-throughput 16S rRNA sequencing combined with UPLC-MS/MS-based untargeted metabolomics was applied to systematically analyze the correlations between the rectal microbial community structure and metabolic profiles and milk quality indicators. The results indicated that milk quality traits exhibited obvious generational differentiation: daily milk yield increased gradually with generation and was significantly higher in F(3) cows than in F(1) cows (p < 0.05), while milk fat rate and milk protein rate showed an upward-then-downward trend and reached the highest level in the F(2) generation. The rectal microbial community underwent directional generational succession, with the relative abundance of Firmicutes continuously increased and Actinobacteriota gradually decreased, and the microbial functions were mainly specialized in carbohydrate and amino acid metabolism. Significant generational differences were also observed in rectal metabolic profiles, and the differential metabolites screened between F(1) and F(3) generations were primarily enriched in steroid hormone biosynthesis, nucleotide sugar metabolism, bile acid metabolism, and amino acid metabolism pathways. Multi-omics correlation analysis further verified that the core rectal microbial genera could regulate the lactation performance of the mammary gland by affecting the abundance of nutritional metabolic substrates and signaling molecules, with metabolites acting as critical mediators in the regulatory relationship between rectal microbes and milk quality traits. This study systematically characterizes the generational succession characteristics of rectal microbes and metabolites in crossbred dairy cows and identifies potential correlative mediating links of rectal metabolites between microbes and milk-quality traits, providing a novel theoretical reference for exploring hindgut microecology to regulate dairy cow milk quality and assist elite breeding. FAU - Wang, Xingyuan AU - Wang X AD - College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China. AD - Animal Disease Prevention and Control Center of Qilihe District, Lanzhou 730050, China. FAU - Huang, Wei AU - Huang W AD - College of Animal Science and Technology, Gansu Agricultural University, Gansu Provincial Technical System for Modern Cold-Arid Characteristic Agricultural Cattle Industry, Lanzhou 730070, China. FAU - Sha, Yuzhu AU - Sha Y AD - College of Animal Science and Technology, Gansu Agricultural University, Gansu Provincial Technical System for Modern Cold-Arid Characteristic Agricultural Cattle Industry, Lanzhou 730070, China. FAU - Shao, Pengyang AU - Shao P AUID- ORCID: 0009-0009-5348-6781 AD - College of Animal Science and Technology, Gansu Agricultural University, Gansu Provincial Technical System for Modern Cold-Arid Characteristic Agricultural Cattle Industry, Lanzhou 730070, China. FAU - Chen, Qianling AU - Chen Q AD - College of Animal Science and Technology, Gansu Agricultural University, Gansu Provincial Technical System for Modern Cold-Arid Characteristic Agricultural Cattle Industry, Lanzhou 730070, China. FAU - He, Yapeng AU - He Y AD - College of Animal Science and Technology, Gansu Agricultural University, Gansu Provincial Technical System for Modern Cold-Arid Characteristic Agricultural Cattle Industry, Lanzhou 730070, China. FAU - Chen, Dingshan AU - Chen D AD - College of Animal Science and Technology, Gansu Agricultural University, Gansu Provincial Technical System for Modern Cold-Arid Characteristic Agricultural Cattle Industry, Lanzhou 730070, China. FAU - Dong, Weitao AU - Dong W AD - College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China. FAU - Liu, Xiu AU - Liu X AUID- ORCID: 0000-0001-6057-2551 AD - College of Animal Science and Technology, Gansu Agricultural University, Gansu Provincial Technical System for Modern Cold-Arid Characteristic Agricultural Cattle Industry, Lanzhou 730070, China. FAU - Zhang, Yong AU - Zhang Y AD - College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China. LA - eng GR - 2025-4/the Science and Technology Program of Qilihe District, Gansu Province/ GR - 26ZDNA004/Major Special Project of Gansu Province/ GR - GSARS‑01/Gansu Agriculture Research System/ GR - ZYGG‑2026‑1/Seed Industry Key Project of Gansu Provincial Department of Agriculture and Rural Affairs/ PT - Journal Article DEP - 20260925 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707599 OTO - NOTNLM OT - Holstein crossbred dairy cows OT - generational succession OT - metabolome OT - milk quality OT - rectal microbiome COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/25 CRDT- 2026/10/27 10:45 PHST- 2026/09/07 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - microorganisms14102169 [pii] AID - microorganisms-14-02169 [pii] AID - 10.3390/microorganisms14102169 [doi] PST - epublish SO - Microorganisms. 2026 Sep 25;14(10):2169. doi: 10.3390/microorganisms14102169. PMID- 42943398 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Sep 24 TI - The Vaginal Microbiome Across the Continuum from HPV Infection to Cervical Cancer: A Narrative Review. LID - 10.3390/microorganisms14102158 [doi] LID - 2158 AB - Persistent high-risk human papillomavirus (HR-HPV) infection serves as the core prerequisite for cervical intraepithelial neoplasia (CIN) and invasive cervical cancer. Accumulated translational evidence confirms that vaginal microecological homeostasis dominated by protective Lactobacillus strains fundamentally governs the initiation, persistence, and spontaneous clearance of HR-HPV infection, while microbial dysbiosis featuring depleted lactobacilli and overgrown anaerobic pathogens creates a pro-inflammatory, immune-suppressive mucosal niche that accelerates viral retention and cervical malignant transformation. We conducted a narrative review of studies published primarily between January 2021 and April 2026. PubMed, Web of Science, CNKI, Wanfang Data, and VIP were searched using terms related to the vaginal microbiome, HR-HPV infection, CIN, cervical cancer, underlying mechanisms, microbial biomarkers, and microbiome-modulating interventions. Relevant peer-reviewed epidemiological, mechanistic, and clinical studies were prioritized, and the manuscript was prepared with reference to the Scale for the Assessment of Narrative Review Articles (SANRA). This review systematically delineates the bidirectional regulatory crosstalk between vaginal microbiota and HR-HPV across the cervical cancer continuum. Dysbiosis disrupts vaginal acidic barriers, reshapes local cytokine networks, impairs antiviral innate immunity, and modulates HPV E6/E7 oncogenic transcription; reciprocally, HR-HPV oncoproteins remodel microbial community structures to sustain a dysbiotic feedback loop. Three core carcinogenic cascades induced by dysbiotic flora are elaborated: epithelial barrier erosion, metabolic reprogramming, and epigenetic rewiring of host and viral genomes. Dynamic microbial characteristics corresponding to all stages of progressive cervical lesions, from transient HPV infection to high-grade CIN and carcinoma, are sorted out. The bench-to-bedside translational progress of microbiome-targeted interventions is summarized, including single/multi-strain probiotic supplementation, engineered lactic acid bacterial therapeutic vaccines, vaginal microbiota transplantation, and traditional Chinese medicine therapy that reconstructs vaginal eubiosis. The clinical application of microbial diversity, strain-level single-nucleotide variants, and taxonomic biomarkers for early screening, efficacy prediction, and postoperative recurrence risk stratification is illustrated. This comprehensive framework with the vaginal microbiome as the core clarifies the internal mechanism of HR-HPV progression along the entire pathway to cervical cancer and provides practical clinical means to block this cascade. Current bottlenecks, including inconsistent probiotic standards, preclinical model limitations, and unresolved gut-vagina microbiome interactions, require further investigation before widespread clinical translation. FAU - Zhou, Xiaodie AU - Zhou X AD - First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China. AD - Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China. FAU - Hu, Jiaqi AU - Hu J AD - First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China. AD - Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China. FAU - Zhou, Ruoyu AU - Zhou R AD - First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China. FAU - Lu, Yi AU - Lu Y AD - First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China. FAU - Du, Xuan AU - Du X AD - First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China. FAU - Chen, Yipei AU - Chen Y AD - First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China. FAU - Ren, Qingling AU - Ren Q AUID- ORCID: 0000-0002-7383-9873 AD - First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China. AD - Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China. FAU - Han, Yue AU - Han Y AD - First Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China. AD - Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, China. LA - eng GR - 82305306/National Natural Science Foundation of China/ GR - k2026yrc72/Jiangsu Provincial Traditional Chinese Medicine Hospital/ GR - PDJH2026014/Jiangsu Provincial Administration of Traditional Chinese Medicine Climbing Program/ GR - Y25003/Jiangsu Provincial Hospital of Traditional Chinese Medicine Project, Special Project for National Medical Center Construction/ PT - Journal Article PT - Review DEP - 20260924 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707505 OTO - NOTNLM OT - Lactobacillus OT - cervical cancer OT - cervical intraepithelial neoplasia OT - high-risk human papillomavirus OT - microbiome biomarker OT - microecological intervention OT - mucosal immunity OT - vaginal dysbiosis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:45 PHST- 2026/08/20 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - microorganisms14102158 [pii] AID - microorganisms-14-02158 [pii] AID - 10.3390/microorganisms14102158 [doi] PST - epublish SO - Microorganisms. 2026 Sep 24;14(10):2158. doi: 10.3390/microorganisms14102158. PMID- 42943397 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Sep 24 TI - Integrated Genetic and Multi-Omic Analyses Reveal Oral Microbial Associations with Brain Structure and Alzheimer's Disease. LID - 10.3390/microorganisms14102157 [doi] LID - 2157 AB - Oral microbial dysbiosis is linked to systemic inflammatory, vascular, and metabolic perturbations, but species-level relationships with human brain structure remain poorly defined. Using two-sample Mendelian randomization, we mapped 438 of 439 oral species with genome-wide association study instruments to 1325 adult brain imaging-derived phenotypes. In total, 87 associations involving 51 taxa and 79 traits passed Benjamini-Hochberg correction across taxa within each imaging-derived phenotype. The childhood and metabolomic screens identified 196 nominal taxon-volume associations and 1118 candidate metabolite-linked paths, respectively, as exploratory findings. Spatial and single-nucleus transcriptomics localized candidate regional and cell-associated variation in brain-structure gene programs. In total, 35 taxa showed nominal genetic associations with Alzheimer's disease. Integration with full-length oral 16S ribosomal ribonucleic acid gene sequencing from a 51-participant case-control cohort prioritized Selenomonas infelix, Capnocytophaga granulosa, and Dialister pneumosintes. In a secondary exploratory analysis, the three-taxon salivary model incorporating educational attainment yielded a leave-one-out cross-validated area under the receiver operating characteristic curve of 0.843. This post-selection estimate is subject to optimism, and the model remains a proof of concept requiring independent external validation. This cross-scale atlas prioritizes microbial and anatomical targets and provides a translational foundation for mechanistic validation and non-invasive neurodegenerative risk markers. FAU - Liu, Yilin AU - Liu Y AD - Department of Neurobiology, School of Basic Medical Sciences, Chongqing Medical University, Chongqing 400010, China. AD - Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), Chongqing Medical University, Chongqing 400010, China. FAU - Feng, Xing AU - Feng X AD - College of Stomatology, Chongqing Medical University, Chongqing 401147, China. AD - Chongqing Key Laboratory of Oral Diseases, Chongqing 401147, China. AD - Chongqing Technology Innovation Center of Smart Dental Medical Devices, Chongqing 401147, China. AD - Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China. AD - Chongqing Municipal Health Commission Key Laboratory of Oral Biomedical Engineering, Chongqing 401147, China. AD - Innovation and Transformation of Dental Medical Devices Engineering Research Center of Chongqing Education Commission of China, Chongqing 401147, China. FAU - Dong, Hongwei AU - Dong H AD - Department of Neurobiology, School of Basic Medical Sciences, Chongqing Medical University, Chongqing 400010, China. AD - Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), Chongqing Medical University, Chongqing 400010, China. FAU - Lan, Tangyue AU - Lan T AD - College of Stomatology, Chongqing Medical University, Chongqing 401147, China. AD - Chongqing Key Laboratory of Oral Diseases, Chongqing 401147, China. AD - Chongqing Technology Innovation Center of Smart Dental Medical Devices, Chongqing 401147, China. AD - Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China. AD - Chongqing Municipal Health Commission Key Laboratory of Oral Biomedical Engineering, Chongqing 401147, China. AD - Innovation and Transformation of Dental Medical Devices Engineering Research Center of Chongqing Education Commission of China, Chongqing 401147, China. FAU - Wu, Jing AU - Wu J AD - College of Stomatology, Chongqing Medical University, Chongqing 401147, China. AD - Chongqing Key Laboratory of Oral Diseases, Chongqing 401147, China. AD - Chongqing Technology Innovation Center of Smart Dental Medical Devices, Chongqing 401147, China. AD - Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China. AD - Chongqing Municipal Health Commission Key Laboratory of Oral Biomedical Engineering, Chongqing 401147, China. AD - Innovation and Transformation of Dental Medical Devices Engineering Research Center of Chongqing Education Commission of China, Chongqing 401147, China. FAU - Wang, Chao AU - Wang C AD - Department of Neurobiology, School of Basic Medical Sciences, Chongqing Medical University, Chongqing 400010, China. AD - Key Laboratory of Major Brain Disease and Aging Research (Ministry of Education), Chongqing Medical University, Chongqing 400010, China. FAU - Zhang, Ximu AU - Zhang X AD - College of Stomatology, Chongqing Medical University, Chongqing 401147, China. AD - Chongqing Key Laboratory of Oral Diseases, Chongqing 401147, China. AD - Chongqing Technology Innovation Center of Smart Dental Medical Devices, Chongqing 401147, China. AD - Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing 401147, China. AD - Chongqing Municipal Health Commission Key Laboratory of Oral Biomedical Engineering, Chongqing 401147, China. AD - Innovation and Transformation of Dental Medical Devices Engineering Research Center of Chongqing Education Commission of China, Chongqing 401147, China. LA - eng GR - 82470988/National Natural Science Foundation of China/ PT - Journal Article DEP - 20260924 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707487 OTO - NOTNLM OT - Mendelian randomization OT - brain structure OT - imaging-derived phenotypes OT - multi-omics integration OT - oral microbiome COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:45 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - microorganisms14102157 [pii] AID - microorganisms-14-02157 [pii] AID - 10.3390/microorganisms14102157 [doi] PST - epublish SO - Microorganisms. 2026 Sep 24;14(10):2157. doi: 10.3390/microorganisms14102157. PMID- 42943392 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Sep 24 TI - In Silico Design and Evaluation of a Multi-Epitope Vaccine Candidate Against Staphylococcus haemolyticus. LID - 10.3390/microorganisms14102152 [doi] LID - 2152 AB - S. haemolyticus is an emerging multidrug-resistant pathogen that frequently causes nosocomial infections, yet no licensed vaccine is currently available for its prevention. In this study, conserved surface-associated proteins were screened to identify B-cell, cytotoxic T-lymphocyte, and helper T-lymphocyte epitopes for the design of a multi-epitope vaccine candidate. The selected epitopes were assembled into a 254-amino acid construct containing human beta-defensin-3, the PADRE sequence, and selected linkers. The resulting construct was predicted to be antigenic, non-allergenic, and non-toxic, with favorable physicochemical properties, structural stability, and solubility. The selected CTL and HTL epitopes provided an estimated global population coverage of 97.5%, indicating broad predicted coverage across different populations. Structural analysis supported the quality of the refined three-dimensional model. Molecular docking predicted favorable interactions between the vaccine construct and TLR2 and TLR4, while 200 ns molecular dynamics simulations indicated that receptor-vaccine interactions were maintained during the simulation period. Codon optimization and in silico cloning further supported the feasibility of recombinant expression in Escherichia coli. Overall, the computational analyses support the proposed multi-epitope construct as a potential vaccine candidate against S. haemolyticus and provide a basis for its further experimental evaluation. FAU - Almatroudi, Ahmad AU - Almatroudi A AUID- ORCID: 0000-0002-1491-6402 AD - Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia. LA - eng PT - Journal Article DEP - 20260924 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707924 OTO - NOTNLM OT - Staphylococcus haemolyticus OT - antimicrobial resistance OT - immunoinformatics OT - multi-epitope vaccine OT - reverse vaccinology COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:45 PHST- 2026/08/30 00:00 [received] PHST- 2026/09/11 00:00 [revised] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - microorganisms14102152 [pii] AID - microorganisms-14-02152 [pii] AID - 10.3390/microorganisms14102152 [doi] PST - epublish SO - Microorganisms. 2026 Sep 24;14(10):2152. doi: 10.3390/microorganisms14102152. PMID- 42943390 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Sep 23 TI - Ogataea Yeast Platform for Recombinant Porcine Interferon-Alpha 1 Production: Toward Alternative Biotherapeutics for Swine Health Management. LID - 10.3390/microorganisms14102150 [doi] LID - 2150 AB - Infectious diseases in swine demand affordable, broad-spectrum antiviral strategies, such as recombinant interferons (IFNs). However, commercial yeast expression platforms often present financial barriers through licensing royalties. To provide a cost-effective regional alternative, we evaluated the domestic, thermotolerant yeast Ogataea polymorpha OP39 for producing recombinant porcine IFNs. Recombinant OP39 strains carrying expression cassettes for types I (poIFN-alpha1), II (poIFN-gamma), and III (poIFN-lambda3) IFNs were constructed and evaluated for their recombinant protein production performance. Following initial screening, the highest-yielding candidate, poIFN-alpha1, was selected for bioprocess optimization in shake flasks and a 5 L fermenter. Results showed that OP39 successfully secreted all three IFN types with a relatively low level of hyperglycosylation. Batch fermentation scale-up of r-poIFN-alpha1 achieved a final protein concentration of 0.45 +/- 0.10 mg/mL. Crucially, the OP39-produced r-poIFN-alpha1 exhibited antiviral efficacy in vitro against economically important porcine viruses including African Swine Fever Virus, Porcine Epidemic Diarrhea Virus and Porcine Reproductive and Respiratory Syndrome Virus. Based on the proof-of-concept results in this study, the OP39 platform can be further developed into a viable, full freedom-to-operate system for local or regional biomanufacturing of biologically active veterinary therapeutics to support livestock health management. FAU - Liwnaree, Benjamas AU - Liwnaree B AD - Veterinary Health Innovation and Management Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand. FAU - Deangphare, Thorntun AU - Deangphare T AUID- ORCID: 0009-0007-8314-0330 AD - Veterinary Health Innovation and Management Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand. FAU - Assana, Chinnawat AU - Assana C AD - Veterinary Health Innovation and Management Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand. FAU - Sansatchanon, Kitisak AU - Sansatchanon K AD - Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand. FAU - Ninrat, Jedsadakorn AU - Ninrat J AD - Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand. FAU - Narkpuk, Jaraspim AU - Narkpuk J AD - Veterinary Health Innovation and Management Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand. FAU - Wanasen, Nanchaya AU - Wanasen N AD - Veterinary Health Innovation and Management Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand. FAU - Puseenam, Aekkachai AU - Puseenam A AD - Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand. FAU - Roongsawang, Niran AU - Roongsawang N AUID- ORCID: 0000-0002-2114-5052 AD - Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand. FAU - Kocharin, Kanokarn AU - Kocharin K AD - Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand. FAU - Jaru-Ampornpan, Peera AU - Jaru-Ampornpan P AD - Veterinary Health Innovation and Management Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani 12120, Thailand. LA - eng GR - C01F660030/Program Management Unit for Competitiveness (PMU-C)/ GR - 4778292 (FF)/Thailand Science Research and Innovation/ PT - Journal Article DEP - 20260923 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707865 OTO - NOTNLM OT - Ogataea polymorpha OT - antivirals OT - porcine interferon OT - recombinant protein expression OT - veterinary biotherapeutics OT - yeast expression system COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/23 CRDT- 2026/10/27 10:45 PHST- 2026/08/06 00:00 [received] PHST- 2026/09/13 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - microorganisms14102150 [pii] AID - microorganisms-14-02150 [pii] AID - 10.3390/microorganisms14102150 [doi] PST - epublish SO - Microorganisms. 2026 Sep 23;14(10):2150. doi: 10.3390/microorganisms14102150. PMID- 42943389 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Sep 23 TI - Validation, Calibration and Transparency, More than Accuracy: An Appraisal of Artificial Intelligence Against Antimicrobial Resistance and a Sustainable, Testable Diagnostic Architecture. LID - 10.3390/microorganisms14102148 [doi] LID - 2148 AB - Artificial intelligence is widely proposed against antimicrobial resistance, but how much of that literature could support clinical deployment is unclear. We searched PubMed (2016-2026) across four domains, retrieving all 2446 records. Of 725 eligible studies, 80 were appraised at full text against the artificial-intelligence appraisal extensions (PROBAST+AI, TRIPOD+AI). Selection favoured externally validated work, so the validation figures are upper bounds. Output grew from 10 studies in 2016 to 142 in 2025; within the appraised set there was no evidence that genuinely external evaluation became more likely (odds ratio 1.08/year, 95% CI 0.89-1.34). Of the 80, 45% were externally validated, 24% released a model a reader could apply, 23% assessed calibration, 16% justified a sample size, and five compared performances across patient characteristics against eight across sites; 8.8% called a random split external validation. Risk of bias was low in 16, unclear in 55 and high in nine, and none of the 18 deep-learning studies reported calibration (95% CI 0-17.6%, a loose bound on 18). We derive the design constraints these impose on a culture-independent diagnostic platform, a projection with no performance data. Validation, calibration and transparency limit this field more than accuracy does: discrimination was reported in every study appraised. FAU - Gulyas-Oldal, Viktor AU - Gulyas-Oldal V AUID- ORCID: 0009-0005-5334-4493 AD - Department of Obstetrics and Gynecology, Faculty of Health and Sport Science, Szechenyi Istvan University, Egyetem ter 1, 9026 Gyor, Hungary. AD - Doctoral School of Regional Sciences and Business Administration, Szechenyi Istvan University, Egyetem ter 1, 9026 Gyor, Hungary. FAU - Galoczi, Imre AU - Galoczi I AUID- ORCID: 0009-0000-2670-4549 AD - Department of Obstetrics and Gynecology, Faculty of Health and Sport Science, Szechenyi Istvan University, Egyetem ter 1, 9026 Gyor, Hungary. AD - Doctoral School of Regional Sciences and Business Administration, Szechenyi Istvan University, Egyetem ter 1, 9026 Gyor, Hungary. FAU - Paschali, Ekaterine AU - Paschali E AUID- ORCID: 0009-0002-9576-5243 AD - Department of Obstetrics and Gynecology, Faculty of Health and Sport Science, Szechenyi Istvan University, Egyetem ter 1, 9026 Gyor, Hungary. AD - Doctoral School of Regional Sciences and Business Administration, Szechenyi Istvan University, Egyetem ter 1, 9026 Gyor, Hungary. FAU - Sallai, Daniel AU - Sallai D AUID- ORCID: 0009-0008-6955-5713 AD - Department of Obstetrics and Gynecology, Faculty of Health and Sport Science, Szechenyi Istvan University, Egyetem ter 1, 9026 Gyor, Hungary. AD - Doctoral School of Regional Sciences and Business Administration, Szechenyi Istvan University, Egyetem ter 1, 9026 Gyor, Hungary. FAU - Dezsi, Csilla AU - Dezsi C AUID- ORCID: 0000-0001-7343-9856 AD - Department of Obstetrics and Gynecology, Faculty of Health and Sport Science, Szechenyi Istvan University, Egyetem ter 1, 9026 Gyor, Hungary. AD - Doctoral School of Regional Sciences and Business Administration, Szechenyi Istvan University, Egyetem ter 1, 9026 Gyor, Hungary. AD - Department of Obstetrics and Gynecology, Faculty of Medicine, University of Szeged, Semmelweis utca 1, 6725 Szeged, Hungary. AD - S.O.S. 24 Kft., Kormanyos utca 5, 6725 Szeged, Hungary. FAU - Meszaros, Peter Tamas AU - Meszaros PT AUID- ORCID: 0009-0001-6799-6987 AD - Department of Obstetrics and Gynecology, Faculty of Health and Sport Science, Szechenyi Istvan University, Egyetem ter 1, 9026 Gyor, Hungary. AD - Doctoral School of Regional Sciences and Business Administration, Szechenyi Istvan University, Egyetem ter 1, 9026 Gyor, Hungary. AD - S.O.S. 24 Kft., Kormanyos utca 5, 6725 Szeged, Hungary. FAU - Virok, Dezso AU - Virok D AD - S.O.S. 24 Kft., Kormanyos utca 5, 6725 Szeged, Hungary. AD - Department of Clinical Microbiology, Faculty of Medicine, University of Szeged, Semmelweis utca 1, 6725 Szeged, Hungary. FAU - Varbiro, Szabolcs AU - Varbiro S AD - Department of Obstetrics and Gynecology, Faculty of Medicine, University of Szeged, Semmelweis utca 1, 6725 Szeged, Hungary. FAU - Nagy, Sandor AU - Nagy S AUID- ORCID: 0000-0002-7054-8333 AD - Department of Obstetrics and Gynecology, Faculty of Health and Sport Science, Szechenyi Istvan University, Egyetem ter 1, 9026 Gyor, Hungary. AD - Doctoral School of Regional Sciences and Business Administration, Szechenyi Istvan University, Egyetem ter 1, 9026 Gyor, Hungary. FAU - Szili, Karoly AU - Szili K AUID- ORCID: 0000-0001-9803-9103 AD - Department of Obstetrics and Gynecology, Faculty of Health and Sport Science, Szechenyi Istvan University, Egyetem ter 1, 9026 Gyor, Hungary. AD - Doctoral School of Regional Sciences and Business Administration, Szechenyi Istvan University, Egyetem ter 1, 9026 Gyor, Hungary. AD - Department of Obstetrics and Gynecology, Faculty of Medicine, University of Szeged, Semmelweis utca 1, 6725 Szeged, Hungary. AD - S.O.S. 24 Kft., Kormanyos utca 5, 6725 Szeged, Hungary. LA - eng PT - Journal Article DEP - 20260923 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707508 OTO - NOTNLM OT - PROBAST OT - TRIPOD OT - antimicrobial resistance OT - antimicrobial stewardship OT - artificial intelligence OT - clinical prediction models OT - machine learning OT - molecular diagnostics OT - qPCR OT - sustainability COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/23 CRDT- 2026/10/27 10:45 PHST- 2026/08/30 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - microorganisms14102148 [pii] AID - microorganisms-14-02148 [pii] AID - 10.3390/microorganisms14102148 [doi] PST - epublish SO - Microorganisms. 2026 Sep 23;14(10):2148. doi: 10.3390/microorganisms14102148. PMID- 42943386 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Sep 23 TI - The Region-Specific Genetic Relationship and Mutation Characteristics of Viral Hemorrhagic Septicemia Virus. LID - 10.3390/microorganisms14102146 [doi] LID - 2146 AB - Since viral hemorrhagic septicemia virus (VHSV) was first identified in rainbow trout, it has evolved into four major genotypes in the process of infecting multiple hosts. The different levels of virulence among genotypes of VHSV are caused by genetic evolution. In this study, the complete genome sequences of global VHSV strains were obtained from the GenBank database and their genetic relationships were analyzed. The maximum likelihood (ML) phylogenetic tree based on the complete genomes of VHSV showed that globally, VHSV strains evolved into two region-specific clades, namely the European clade and the North American clade. North American VSHV strains are more conserved than European strains. Both European and North American strains are subjected to purifying selection. All VHSV proteins are gathered into their region-specific clusters in genetic distance space. Furthermore, North American and Asian strains have organized glycosylation sites in their G protein. An early Japanese strain, KRRV9822, which clusters into the North American clade, has undergone potential recombination. Together, the region-specific genetic relationship and mutation characteristics of global VHSV strains were revealed with a crucial perspective for epidemiological research on VHSV. FAU - Lian, Zhengmin AU - Lian Z AUID- ORCID: 0009-0000-9168-2446 AD - Institute of Livestock, Grass, and Green Agriculture, Gansu Academy of Agricultural Sciences, Lanzhou 730020, China. FAU - Zhang, Jian AU - Zhang J AD - Institute of Livestock, Grass, and Green Agriculture, Gansu Academy of Agricultural Sciences, Lanzhou 730020, China. FAU - Zhou, Changqing AU - Zhou C AUID- ORCID: 0000-0001-9456-7021 AD - Institute of Livestock, Grass, and Green Agriculture, Gansu Academy of Agricultural Sciences, Lanzhou 730020, China. LA - eng GR - 2026GAAS26/Gansu Academy of Agricultural Sciences Institutional Scientific Research Program Innovation Talent Introduction and Training Special Project/ PT - Journal Article DEP - 20260923 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707813 OTO - NOTNLM OT - genetic evolution OT - recombination OT - region specificity OT - viral hemorrhagic septicemia virus COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/23 CRDT- 2026/10/27 10:45 PHST- 2026/07/31 00:00 [received] PHST- 2026/09/12 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - microorganisms14102146 [pii] AID - microorganisms-14-02146 [pii] AID - 10.3390/microorganisms14102146 [doi] PST - epublish SO - Microorganisms. 2026 Sep 23;14(10):2146. doi: 10.3390/microorganisms14102146. PMID- 42943385 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Sep 23 TI - Enhancing the Resistance of Soybean Plants to Waterlogging Stress Using Gamma-Irradiated Priestia megaterium. LID - 10.3390/microorganisms14102145 [doi] LID - 2145 AB - The rhizosphere of Fabaceae contains plant growth-promoting rhizobacteria (PGPR) that regulate phytohormone balance, nutrient acquisition, and biofilm formation, thereby contributing to plant development and stress tolerance. Although Indole-3-acetic acid (IAA) and exopolysaccharides (EPS) are well-established PGPR-associated factors, their combined contributions to waterlogging tolerance in soybean remain unclear. In this study, Bacillus-like PGPR were isolated from the rhizosphere of soybean plants cultivated in field soil, and Priestia megaterium and P. aryabhattai were identified among Bacillus-like bacteria based on strong EPS production, a PGPR-associated trait. The P. megaterium isolate S135-A1-2 was subjected to gamma-ray irradiation to generate mutants, from which mutants #6 (increased EPS), #8 (increased IAA), and #12 (increased EPS and IAA) were selected for further analysis. Mutant #8 produced 4.48 mug/mL IAA, a 3.6-fold increase over the parental strain, and protease and siderophore activities were also evaluated as additional PGPR-associated traits. Their plant growth-promoting traits were evaluated using pot assays under waterlogging conditions. Inoculation with mutants #6 and #8 increased lateral root development and root biomass relative to the parental strain, whereas mutant #12 showed a distinct response pattern associated with dual-trait enhancement under waterlogging stress. After 7 days of waterlogging, mutant #12 showed higher chlorophyll-related values than mutants #6 and #8. These results indicate that gamma-irradiated P. megaterium mutants can differentially modify soybean root system responses to flooding stress, supporting gamma-ray irradiation as a useful approach for developing PGPR with improved functions for agricultural applications under waterlogging stresses. FAU - Wang, Sin-Tian AU - Wang ST AUID- ORCID: 0000-0002-1341-9930 AD - Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan. FAU - Chen, Lih-Geeng AU - Chen LG AUID- ORCID: 0000-0003-2407-6782 AD - Department of Microbiology, Immunology and Biopharmaceuticals, National Chiayi University, Chiayi 600, Taiwan. FAU - Shu, Hung-Yu AU - Shu HY AUID- ORCID: 0000-0002-2213-9324 AD - Department of Bioscience Technology, Chang Jung Christian University, Tainan 711, Taiwan. FAU - Lu, Shih-Yin AU - Lu SY AD - Department of Microbiology, Immunology and Biopharmaceuticals, National Chiayi University, Chiayi 600, Taiwan. FAU - Chen, Yu-Cheng AU - Chen YC AD - Department of Microbiology, Immunology and Biopharmaceuticals, National Chiayi University, Chiayi 600, Taiwan. FAU - Su, Min-Chang AU - Su MC AD - Department of Isotope Application Research, National Atomic Research Institute, Taoyuan 325, Taiwan. FAU - Wang, Shao-Hung AU - Wang SH AUID- ORCID: 0000-0002-6824-0372 AD - Department of Microbiology, Immunology and Biopharmaceuticals, National Chiayi University, Chiayi 600, Taiwan. LA - eng GR - 109-NU-E-415-001-NU/National Science and Technology Council/ GR - 111-NU-E-415-001-NU/National Science and Technology Council/ PT - Journal Article DEP - 20260923 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707707 OTO - NOTNLM OT - Priestia megaterium OT - gamma-ray irradiation OT - plant growth-promoting rhizobacteria (PGPR) OT - waterlogging stress COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/23 CRDT- 2026/10/27 10:45 PHST- 2026/07/14 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - microorganisms14102145 [pii] AID - microorganisms-14-02145 [pii] AID - 10.3390/microorganisms14102145 [doi] PST - epublish SO - Microorganisms. 2026 Sep 23;14(10):2145. doi: 10.3390/microorganisms14102145. PMID- 42943382 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Sep 23 TI - Post-Hatch Starvation in Black Soldier Fly Larvae: Implications for Growth, Nutritional Quality, and Multi-Omics Profiles Across Rearing Substrates. LID - 10.3390/microorganisms14102142 [doi] LID - 2142 AB - The black soldier fly (BSF, Hermetia illucens L.) has gained interest for its ability to convert organic waste into high-quality protein. The eggs of BSF are typically sold in fixed quantities, which often does not match the fluctuating waste input of processing facilities; post-hatch starvation offers a practical strategy to buffer this supply-demand mismatch. However, how post-hatch starvation affects larval development and nutrient accumulation remains unclear. After being reared to approximately 5 mg, neonate larvae were subjected to starvation periods of 0, 7, 14, and 21 days in wheat bran. After starvation, larvae were reared on two substrates: kitchen waste and chicken manure, to evaluate how starvation time influences larval nutrition, growth, microbiota and metabolome. With prolonged starvation, larvae reared on kitchen waste showed increased crude protein and total amino acids, while crude fat exhibited a biphasic pattern, decreasing initially before rising again. The proportion of C12:0 fatty acid consistently increased. In contrast, larvae reared on chicken manure showed an increase in crude fat content after 21 days, alongside decreased total amino acids and increased C12:0. After 21 days of starvation, the survival rate dropped to 68%. The final fresh body weight and length of larvae in both groups increased with prolonged starvation duration. Substrate conversion efficiency peaked at 7 days of starvation across both substrates, whereas substrate reduction rates progressively declined with extended starvation, reaching the lowest values at 21 days. Microbiota analysis of the larval intestines revealed that the gut bacterial community underwent selective remodeling in response to substrate type and starvation duration. Specifically, kitchen waste, rich in fermentable carbohydrates and proteins, promoted an increased relative abundance of Ignatzschineria to decompose nitrogenous organic matter and supply energy. Conversely, chicken manure, which is higher in refractory organic carbon, enriched Moheibacter to degrade complex compounds such as chitin and cellulose. Metabolomic changes included enrichment in tryptophan, linoleic acid, and alpha-linolenic acid metabolism pathways. Ultimately, the studies revealed that 7 days is the optimal fasting duration to maximize bioconversion efficiency and larval production, while fasting for up to 14 days remains a safe preservation limit before survival rates significantly decline. These findings clarify how post-hatch starvation shapes BSF larval composition, growth, and molecular profiles, aiding the optimization of preservation duration and the enhancement of bioconversion efficiency and larval nutritional quality of BSF. FAU - Guo, Jianlai AU - Guo J AD - Henan Key Laboratory of Unconventional Feed Resources Innovative Utilization, College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China. FAU - Jiao, Minghui AU - Jiao M AD - College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China. FAU - Li, Yuting AU - Li Y AD - College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China. FAU - Shi, Huibin AU - Shi H AD - Henan Key Laboratory of Unconventional Feed Resources Innovative Utilization, College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China. FAU - Li, Jun AU - Li J AUID- ORCID: 0000-0002-3758-4084 AD - Henan Key Laboratory of Unconventional Feed Resources Innovative Utilization, College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China. FAU - Ji, Xiangbo AU - Ji X AD - Henan Key Laboratory of Unconventional Feed Resources Innovative Utilization, College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China. FAU - Zhang, Weixian AU - Zhang W AD - Henan Key Laboratory of Unconventional Feed Resources Innovative Utilization, College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China. FAU - Quan, Kai AU - Quan K AUID- ORCID: 0000-0002-0015-8663 AD - Henan Key Laboratory of Unconventional Feed Resources Innovative Utilization, College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China. FAU - Jiang, Yibao AU - Jiang Y AD - College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China. FAU - Liu, Kun AU - Liu K AUID- ORCID: 0009-0006-8053-5591 AD - Henan Key Laboratory of Unconventional Feed Resources Innovative Utilization, College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China. LA - eng GR - 2022HNUAHEDF002/The Doctoral research start-up fund of Henan University of Animal Husbandry and Economy/ GR - K2070479/The Project of Henan Provincial Animal Husbandry Industry Sci-Tech Commissioner Service Group/ PT - Journal Article DEP - 20260923 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707832 OTO - NOTNLM OT - black soldier fly OT - growth performance OT - nutritional composition OT - omics technologies OT - starvation duration COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/23 CRDT- 2026/10/27 10:45 PHST- 2026/09/09 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - microorganisms14102142 [pii] AID - microorganisms-14-02142 [pii] AID - 10.3390/microorganisms14102142 [doi] PST - epublish SO - Microorganisms. 2026 Sep 23;14(10):2142. doi: 10.3390/microorganisms14102142. PMID- 42943378 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Sep 23 TI - Biochar Application Improves Eggplant Yield in Association with Changes in Soil Microbial Communities and Soil Multifunctionality. LID - 10.3390/microorganisms14102139 [doi] LID - 2139 AB - Biochar is widely used to improve soil quality and crop productivity; however, the relationships among biochar addition, soil multifunctionality (SMF), microbial communities, and crop yield remain unclear. A field experiment was conducted from May to September 2025 in a continuous eggplant (Solanum melongena L.) cropping system established on Mollisols. Four biochar application rates (0, 5.40, 6.75, and 8.10 Mg ha(-1)) were evaluated using a randomized complete block design with three replicates. Biochar addition significantly affected eggplant growth, selected photosynthetic traits, root development, and yield, with the most consistent positive responses observed at 6.75 Mg ha(-1). Biochar also altered soil physicochemical and biological properties, with these effects being more pronounced in the topsoil and at the fruiting stage. SMF was positively correlated with yield, and this relationship was stronger at the fruiting stage than at the flowering stage. Random forest analysis showed that yield was strongly associated with soil biological properties, particularly extracellular enzyme activities involved in carbon, nitrogen, and phosphorus acquisition. Structural equation modeling further examined depth-specific statistical associations among biochar addition, microbial communities, SMF, and yield; topsoil bacterial communities and subsoil fungal communities showed the strongest associations with SMF. Overall, the application of 6.75 Mg ha(-1) biochar produced the most consistent improvements in SMF and eggplant productivity. FAU - Liu, Fangtian AU - Liu F AD - College of Science, Jiamusi University, Jiamusi 154007, China. FAU - Kang, Qijin AU - Kang Q AD - College of Science, Jiamusi University, Jiamusi 154007, China. FAU - Song, Chunxiang AU - Song C AD - College of Science, Jiamusi University, Jiamusi 154007, China. FAU - Wu, Yude AU - Wu Y AD - College of Science, Jiamusi University, Jiamusi 154007, China. FAU - Li, Fu AU - Li F AD - College of Science, Jiamusi University, Jiamusi 154007, China. LA - eng GR - PL2024C005/Natural Science Foundation Project of Heilongjiang Province/ PT - Journal Article DEP - 20260923 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707460 OTO - NOTNLM OT - biochar OT - eggplant yield OT - microbial community OT - mollisols OT - soil multifunctionality COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/23 CRDT- 2026/10/27 10:45 PHST- 2026/08/16 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - microorganisms14102139 [pii] AID - microorganisms-14-02139 [pii] AID - 10.3390/microorganisms14102139 [doi] PST - epublish SO - Microorganisms. 2026 Sep 23;14(10):2139. doi: 10.3390/microorganisms14102139. PMID- 42943372 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Sep 22 TI - Global Genomic Analysis of Brucella spp. Reveals Antimicrobial Resistance, Virulence, and Mobile Genetic Element Profiles from a One Health Perspective. LID - 10.3390/microorganisms14102132 [doi] LID - 2132 AB - Brucellosis is a globally distributed zoonotic disease that poses a severe threat to human and animal health. However, the population structure, evolutionary dynamics, and antimicrobial resistance (AMR)-associated genomic characteristics of Brucella spp. across hosts, regions, and time periods remain insufficiently characterized. A total of 1760 publicly available Brucella spp. genomes were retrieved. After metadata screening, 701 genomes lacking required metadata were excluded, and 1059 genomes were retained for downstream analyses. Analyses included annotation of antimicrobial resistance genes (ARGs), virulence factors (VFs), and mobile genetic elements (MGEs), together with multilocus sequence typing (MLST), pan-genome analysis, and phylogenomic reconstruction. MLST assigned 836 genomes to 49 sequence types, whereas 223 remained unclassified. Environmental isolates showed relatively higher ARG annotation abundance in the analyzed dataset. Peptide resistance-associated annotations predominated (91.6%), largely due to Bsui_mprF and bacA, followed by beta-lactam (4.3%) and aminoglycoside (2.6%), reflecting resistance-associated genomic annotations rather than phenotypically confirmed AMR. Several ARGs occurred in MGE-associated genomic contexts, suggesting potential mobility. VF profiles were broadly conserved overall, while selected determinants varied among species, geographic regions, and sources. Phylogenomic analysis revealed close relationships among isolates from different hosts, with ST8 and ST11 representing predominant lineages. These findings support integrated genomic surveillance across human, animal, and environmental sources. FAU - Han, Chenyang AU - Han C AUID- ORCID: 0009-0004-0733-7738 AD - Xinjiang Production and Construction Corps Key Laboratory for Efficient Utilization of Non-Grain Feed Resources, International Joint Research Center for Healthy Animal Farming, College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. FAU - He, Tao AU - He T AUID- ORCID: 0000-0001-8647-5178 AD - Xinjiang Production and Construction Corps Key Laboratory for Efficient Utilization of Non-Grain Feed Resources, International Joint Research Center for Healthy Animal Farming, College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. FAU - Liang, Yafei AU - Liang Y AD - Xinjiang Production and Construction Corps Key Laboratory for Efficient Utilization of Non-Grain Feed Resources, International Joint Research Center for Healthy Animal Farming, College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. FAU - Yang, Bo AU - Yang B AD - Xinjiang Production and Construction Corps Key Laboratory for Efficient Utilization of Non-Grain Feed Resources, International Joint Research Center for Healthy Animal Farming, College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. FAU - Gong, Wenjuan AU - Gong W AD - Xinjiang Production and Construction Corps Key Laboratory for Efficient Utilization of Non-Grain Feed Resources, International Joint Research Center for Healthy Animal Farming, College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. FAU - Hao, Haihong AU - Hao H AUID- ORCID: 0000-0003-0199-5700 AD - National Reference Laboratory of Veterinary Drug Residues, Huazhong Agricultural University, Wuhan 430070, China. FAU - Sheng, Jinliang AU - Sheng J AD - Xinjiang Production and Construction Corps Key Laboratory for Efficient Utilization of Non-Grain Feed Resources, International Joint Research Center for Healthy Animal Farming, College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. FAU - Pan, Yu AU - Pan Y AD - Xinjiang Production and Construction Corps Key Laboratory for Efficient Utilization of Non-Grain Feed Resources, International Joint Research Center for Healthy Animal Farming, College of Animal Science and Technology, Shihezi University, Shihezi 832003, China. LA - eng GR - No. 2024AB034/Xinjiang Production and Construction Corps/ GR - No. RCZK202458/Shihezi University/ PT - Journal Article DEP - 20260922 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707526 OTO - NOTNLM OT - Brucella spp. OT - antimicrobial resistance OT - global genomic surveillance OT - virulence factors COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:45 PHST- 2026/08/06 00:00 [received] PHST- 2026/09/11 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - microorganisms14102132 [pii] AID - microorganisms-14-02132 [pii] AID - 10.3390/microorganisms14102132 [doi] PST - epublish SO - Microorganisms. 2026 Sep 22;14(10):2132. doi: 10.3390/microorganisms14102132. PMID- 42943366 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2607 (Print) IS - 2076-2607 (Electronic) IS - 2076-2607 (Linking) VI - 14 IP - 10 DP - 2026 Sep 22 TI - Deciphering Macroalgal Microbiomes: Metagenomic Insights, Ecological Roles and Bioprospecting Potential. LID - 10.3390/microorganisms14102127 [doi] LID - 2127 AB - Macroalgae host complex microbial communities that influence surface colonization, development, nutrient transformation, carbohydrate turnover, environmental responses, and the production of potentially valuable metabolites. Advances in shotgun metagenomics and genome-resolved analysis have greatly expanded access to this functional diversity, but they have also increased the risk of conflating genomic potential with biological activity or host benefit. This narrative review evaluates the bacterial component of macroalgal microbiomes-the component for which mechanistic and experimentally validated evidence is most extensive-through a framework that separates what has been proven from what is merely predicted, distinguishing functional prediction from expression, biochemical activity, metabolite exchange, host response, and experimental causality. Particular attention is given to bacteria-dependent morphogenesis, metabolic complementarity, environmental acclimation, carbohydrate-active enzymes and polysaccharide-utilization loci, biosynthetic gene clusters, and the translational potential of seaweed-associated microorganisms. Comparative perspectives from microalgal and cyanobacterial systems are used to identify principles that generalize across algal microbiomes while emphasizing the distinctive spatial and chemical complexity of macroalgal surfaces. Invasive and bloom-forming macroalgae provide an additional ecological context in which microbial functional redundancy, recruitment flexibility, and dominant populations may shape whole-community profiles. An illustrative, hypothesis-generating taxon-attribution case study, based on single metagenomic datasets from Rugulopteryx okamurae and Sargassum sp. holobionts in the Azores, which confounds host identity with sampling location, time, and thallus condition, shows that the disproportionate genomic contribution of the genus Cobetia measurably alters the interpretation of a specific subset of community-level KEGG pathway contrasts, while the majority of pathway-level and taxon-specific signals discussed retain their direction after this taxon's exclusion and residual-community renormalization. Together, these analyses argue that macroalgal microbiome research should move beyond inventories of predicted functions toward explicit taxonomic attribution, experimental validation, and ecological context. Such integration will be essential for establishing which microbial functions are ecologically consequential and which can be translated reproducibly into biotechnology. FAU - Moreira, Joao AU - Moreira J AUID- ORCID: 0009-0000-5621-1315 AD - OKEANOS-Institute of Marine Sciences, University of the Azores, 9901-862 Horta, Portugal. AD - Faculty of Science and Technology, University of the Azores, 9900-862 Horta, Portugal. FAU - Bettencourt, Raul AU - Bettencourt R AUID- ORCID: 0000-0001-6866-7029 AD - OKEANOS-Institute of Marine Sciences, University of the Azores, 9901-862 Horta, Portugal. AD - Faculty of Science and Technology, University of the Azores, 9900-862 Horta, Portugal. LA - eng GR - UID/05634/2025 (DOI: 10.54499/UID/05634/2025); UID/PRR/05634/2025 (DOI: 10.54499/UID/PRR/05634/2025); UID/PRR2/05634/2025 (DOI: 10.54499/UID/PRR2/05634/2025)/Fundacao para a Ciencia e Tecnologia/ GR - PROSCIENTIA Incentive System-project M1.1.A/FUNC.UI&D/014/2025/Regional Directorate for Science, Innovation and Development of the Azores Government-PROSCIENTIA Incentive System/ GR - ACORES-01-0145-FEDER-000138/European Regional Development Fund (ERDF)/ GR - M3.1.b context emp/F/017/2024/Fundo Regional para a Ciencia e Tecnologia/ PT - Journal Article PT - Review DEP - 20260922 PL - Switzerland TA - Microorganisms JT - Microorganisms JID - 101625893 PMC - PMC13707482 OTO - NOTNLM OT - CAZymes OT - Cobetia OT - bioprospecting OT - biosynthetic gene clusters OT - holobiont OT - macroalgae OT - metagenomics OT - microbiome OT - polysaccharide utilization loci OT - taxon attribution COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:45 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - microorganisms14102127 [pii] AID - microorganisms-14-02127 [pii] AID - 10.3390/microorganisms14102127 [doi] PST - epublish SO - Microorganisms. 2026 Sep 22;14(10):2127. doi: 10.3390/microorganisms14102127. PMID- 42943361 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 21 TI - Computational Assessment of CKP-25 Binding at Receptor-Recognition Interfaces Across Diverse Human Coronaviruses. LID - 10.3390/ph19101664 [doi] LID - 1664 AB - Background/Objectives: The continued emergence of novel coronaviruses motivates the search for small molecules that bind conserved features of the spike receptor-recognition interface across diverse species. CKP-25 was recently reported as a small-molecule inhibitor of the SARS-CoV-2 spike receptor-binding domain. This study aimed to assess computationally whether CKP-25 can maintain stable binding at receptor-recognition interfaces across phylogenetically diverse human coronaviruses. Methods: CKP-25 was docked to the spike receptor-binding regions of Middle East respiratory syndrome coronavirus (MERS-CoV), severe acute respiratory syndrome coronavirus 1 (SARS-CoV-1), and human coronaviruses NL63, 229E, and HKU1 (HCoV-NL63, HCoV-229E, and HCoV-HKU1). SARS-CoV-2 (PDB 6M0J), for which CKP-25 has documented antiviral activity, was processed identically and included as an experimentally validated reference system. Each selected complex was subjected to three independent 100 ns all-atom molecular dynamics (MD) simulations initialized with different random velocities. Binding stability was evaluated using protein and ligand root-mean-square deviation (RMSD), residue flexibility, radius of gyration, protein-ligand contact persistence, polar interactions, and Molecular Mechanics-Generalized Born Surface Area (MM-GBSA) calculations. Results: CKP-25 was retained at the targeted receptor-recognition interface in all three replicates for four of the five test targets-MERS-CoV, HCoV-NL63, SARS-CoV-1, and HCoV-HKU1-each of which reproducibly engaged the same aromatic and hydrophobic anchor residues in every independent run. HCoV-229E was the sole exception, the ligand relocating from its docked site in two of three replicates. Ligand RMSD plateaus were generally low, mostly below 2.0 A. Contact analyses indicated a shared dependence on shape-complementary van der Waals packing involving hydrophobic and aromatic residues, including tyrosine- and tryptophan-rich anchoring environments. MM-GBSA estimates, averaged across replicates, ranged from -24.28 to -34.57 kcal/mol. No test target differed from the SARS-CoV-2 reference (-29.15+/-3.46 kcal/mol) by more than the replicate-to-replicate spread, with MERS-CoV and HCoV-NL63 giving the most favorable means. Conclusions: CKP-25 remained at the receptor-recognition interface of four of the five human coronaviruses tested, and it did so in all three replicates. In the SARS-CoV-2 control, the same pipeline independently reproduced the experimentally established binding mode, which supports the reliability of these predictions. HCoV-229E was the exception: the ligand left the docked site in two of the three replicates, and no contact was reproducible across all three repetitions. Experimental binding measurements and cell-based entry assays are required to test these predictions. FAU - Papadopoulos, Angelos-Michail AU - Papadopoulos AM AUID- ORCID: 0009-0000-8117-312X AD - Information Technologies Institute, Centre for Research and Technology Hellas (CERTH), 6th km Charilaou-Thermi Road, Thermi, 57001 Thessaloniki, Greece. AD - Grupo de Aplicacion de Telecomunicaciones Visuales, Information Processing and Telecommunications Center, Universidad Politecnica de Madrid, 28040 Madrid, Spain. FAU - Petrakis, Spyros AU - Petrakis S AUID- ORCID: 0000-0001-9094-3480 AD - Institute of Applied Biosciences, Centre for Research and Technology Hellas (CERTH), 6th km Charilaou-Thermi Road, Thermi, 57001 Thessaloniki, Greece. FAU - Alvarez, Federico AU - Alvarez F AUID- ORCID: 0000-0001-7400-9591 AD - Grupo de Aplicacion de Telecomunicaciones Visuales, Information Processing and Telecommunications Center, Universidad Politecnica de Madrid, 28040 Madrid, Spain. FAU - Daras, Petros AU - Daras P AUID- ORCID: 0000-0003-3814-6710 AD - Information Technologies Institute, Centre for Research and Technology Hellas (CERTH), 6th km Charilaou-Thermi Road, Thermi, 57001 Thessaloniki, Greece. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708399 OTO - NOTNLM OT - CKP-25 OT - MM-GBSA OT - coronavirus OT - in silico binding assessment OT - molecular docking OT - molecular dynamics OT - receptor-binding interface COIS- S. Petrakis and P. Daras are inventors of the European Patent Application 24719547.2-1102 describing the activity of CKP-25 against SARS-CoV-2. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:45 PHST- 2026/08/24 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ph19101664 [pii] AID - pharmaceuticals-19-01664 [pii] AID - 10.3390/ph19101664 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 21;19(10):1664. doi: 10.3390/ph19101664. PMID- 42943360 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 21 TI - Trimetazidine in Heart Failure: From Metabolic Modulation to Clinical Outcomes-A Narrative Review. LID - 10.3390/ph19101663 [doi] LID - 1663 AB - Heart Failure (HF) remains a leading cause of morbidity and mortality worldwide despite major advances in guideline-directed medical therapy. Metabolic remodeling with impaired myocardial energy utilization has emerged as an important therapeutic target in HF. Trimetazidine, a metabolic modulator that partially inhibits fatty acid beta-oxidation and favours glucose oxidation, enhances myocardial energy efficiency without changing systemic hemodynamics. This narrative review summarizes the molecular mechanisms underlying the cardioprotective effects of trimetazidine and critically evaluates the available clinical evidence supporting its use in HF. Experimental studies have shown that trimetazidine increases ATP production, maintains mitochondrial function, decreases oxidative stress, inhibits apoptosis of cardiomyocytes, regulates autophagy, and reduces myocardial inflammation and fibrosis. Clinical studies and meta-analyses have shown that adjunctive therapy with trimetazidine has been associated with improved left ventricular ejection fraction, ventricular remodeling, exercise capacity, New York Heart Association functional class, biomarkers of myocardial injury, and quality of life in patients with heart failure with reduced ejection fraction. The available evidence also indicates reductions in cardiovascular hospitalization and possibly all-cause mortality, but these findings are based primarily on relatively small randomized trials and pooled analyses, and need confirmation in adequately powered multicenter studies. The potential role of trimetazidine alongside contemporary therapies, including sodium-glucose cotransporter-2 inhibitors and other guideline-directed treatments, is also discussed. Overall, trimetazidine appears to be a promising adjunctive metabolic therapy that may complement existing pharmacological strategies in selected patients with heart failure (Central Illustration). Further large-scale clinical trials are needed to better define its place in contemporary HF management. FAU - Pradhan, Akshyaya AU - Pradhan A AUID- ORCID: 0000-0002-2360-7580 AD - Department of Cardiology, King George's Medical University, Lucknow 226003, Uttar Pradesh, India. FAU - Prajapathi, Sudesh AU - Prajapathi S AUID- ORCID: 0009-0000-8447-6118 AD - Department of Cardiology, All India Institute of Medical Sciences, Bhopal 462020, Madhya Pradesh, India. FAU - Mahajan, Kunal AU - Mahajan K AUID- ORCID: 0000-0001-9378-6505 AD - Himachal Heart Institute, Mandi 175021, Himachal Pradesh, India. FAU - Batta, Akash AU - Batta A AUID- ORCID: 0000-0002-7606-5826 AD - Department of Cardiology, Dayanand Medical College and Hospital (DMCH), Ludhiana 141001, Punjab, India. FAU - Perrone, Marco Alfonso AU - Perrone MA AUID- ORCID: 0000-0002-0511-2621 AD - Division of Cardiology and CardioLab, Department of Clinical Sciences and Translational Medicine, University of Rome Tor Vergata, 00133 Rome, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708437 OTO - NOTNLM OT - cardioprotection OT - glucose oxidation OT - heart failure OT - heart failure with reduced ejection fraction OT - metabolic therapy OT - mitochondrial dysfunction OT - myocardial energy metabolism OT - oxidative stress OT - trimetazidine OT - ventricular remodeling COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:45 PHST- 2026/06/29 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ph19101663 [pii] AID - pharmaceuticals-19-01663 [pii] AID - 10.3390/ph19101663 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 21;19(10):1663. doi: 10.3390/ph19101663. PMID- 42943357 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 21 TI - Nephron-Sparing Strategies for Tumor-Bearing Horseshoe Kidney: From Surgeries to Pharmaceuticals. LID - 10.3390/ph19101660 [doi] LID - 1660 AB - Background/Objectives: Horseshoe kidney (HSK) is a type of congenital renal fusion anomaly with extreme anatomic complexity. The malrotation of kidneys and ureters and malformation and altered isthmus and vascular supply may boost several renal and extrarenal complications. Organ preservation of tumor-bearing HSK is rather difficult and only available in a small minority of patients. Methods/Results: This review introduces new advances for nephron-sparing surgical strategies for tumor-bearing HSK, such as super-selective artery clamping, ultrasound-guided renal artery balloon catheter occluded hybrid partial nephrectomy, and focal therapies. It also puts forward novel pharmaceuticals for kidney function preservation to alleviate ischemia-reperfusion injury and medications for neoadjuvant therapies. Conclusions: HSK possesses unique etiology, complex anatomic characteristics, and hidden complications. Renal function preservation is extremely important for tumor-bearing HSK. For long-term high-quality lifespan, precise preoperative planning, surgeon's versatility with multiple surgical techniques, and novel pharmaceuticals to avoid IRI are promising strategies for nephron-sparing surgery. FAU - He, Meng-Zhen AU - He MZ AD - Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China. AD - Department of Nursing, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China. FAU - Zhou, Hui AU - Zhou H AUID- ORCID: 0000-0002-1004-9620 AD - Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China. AD - Hubei Provincial Clinical Research Center for Minimally Invasive Treatment of Urology, Wuhan 430030, China. LA - eng GR - 82002701/National Natural Science Foundation of China/ PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708348 OTO - NOTNLM OT - horseshoe kidney OT - ischemia-reperfusion injury OT - organ preservation OT - partial nephrectomy OT - renal cell carcinoma COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:45 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ph19101660 [pii] AID - pharmaceuticals-19-01660 [pii] AID - 10.3390/ph19101660 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 21;19(10):1660. doi: 10.3390/ph19101660. PMID- 42943353 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 20 TI - From Design to Synergy: Triazine-Triazole Hybrids as Anti-Breast Cancer Leads Targeting Survival and Invasion Pathways for Augmenting Lapatinib Efficacy. LID - 10.3390/ph19101656 [doi] LID - 1656 AB - Background/Objectives: Breast cancer is the most common cancer diagnosed and the leading cause of cancer-related deaths among women, ranking fourth overall in both sexes. Its treatment is difficult due to drug resistance and side effects from conventional therapies. Developing multitargeted agents and combination approaches offer a promising way to overcome these challenges. This study details the design and testing of new triazole-triazine hybrids as potential anti-breast cancer drugs, exploring their use in combination with lapatinib to enhance its efficacy in breast cancer. Methods: Sixteen triazine-triazole hybrid compounds with amide and ureido linkers were synthesized via click and coupling protocols and tested for their cytotoxic effects on MCF-7 cells using an MTT assay. The most effective compound was evaluated for its inhibitory effect against AXL using an ADP-Glo kinase assay, then examined in combination with lapatinib on cellular and molecular levels. In vitro experiments included chemoinvasion assays for metastasis, flow-cytometric analysis of apoptosis, and ELISA to evaluate the molecular mechanisms of promising hits. Molecular docking studies were conducted to simulate the potential target-hit interactions and predict the plausible structural determinants of activity, paving the way for further lead optimization. Results: Compound 6e demonstrated the highest activity (IC(50) = 24.58 muM) and good selectivity (SI = 7.87). The combination therapy showed a synergistic effect and increased tumor sensitivity to lapatinib. Biological tests indicated inhibition of the AXL enzyme, downregulation of key pathways (AKT, NF-kappaB, STAT3, ERK), decreased levels of VEGF, MMP9, and cyclin D1, and increased caspase-3 activity, suggesting apoptosis, which was strengthened by the flow-cytometric analysis findings. Docking results supported multitarget interactions. Conclusions: Compound 6e is a promising multitarget anti-breast cancer candidate with synergistic potential in combination with lapatinib, warranting further in vivo studies. FAU - Ali, Omnia M AU - Ali OM AUID- ORCID: 0009-0006-6389-2722 AD - Department of Medicinal Chemistry, Faculty of Pharmacy, Minia University, Minia 61519, Egypt. AD - Department of Medicinal Chemistry, College of Pharmacy, Arab Academy for Science, Technology and Maritime Transport, El-Alamein 5060305, Egypt. FAU - Abbas, Samar Hafez AU - Abbas SH AUID- ORCID: 0000-0002-9586-2644 AD - Department of Medicinal Chemistry, Faculty of Pharmacy, Minia University, Minia 61519, Egypt. AD - Department of Medicinal Chemistry, Faculty of Pharmacy, Minia National University, New Minia 61768, Egypt. FAU - Shehat, Micheal G AU - Shehat MG AUID- ORCID: 0000-0002-0418-456X AD - Department of Microbiology and Immunology, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt. FAU - Helmy, Maged W AU - Helmy MW AUID- ORCID: 0000-0003-0375-6762 AD - Pharmacology and Toxicology Department, Faculty of Pharmacy, Damanhour University, Damanhour 22511, Egypt. AD - Pharmacology and Toxicology Department, Faculty of Pharmacy, Damanhour National University, Damanhour 22511, Egypt. FAU - Aly, Moustafa H AU - Aly MH AUID- ORCID: 0000-0003-1966-3755 AD - College of Engineering and Technology, Arab Academy for Science, Technology and Marine Transport, El-Alamein 5060305, Egypt. FAU - Teleb, Mohamed AU - Teleb M AD - Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Alexandria University, Alexandria 21521, Egypt. AD - Department of Medicinal Chemistry, Faculty of Pharmacy, Alamein International University (AIU), El-Alamein 5060310, Egypt. FAU - Khattab, Sherine N AU - Khattab SN AUID- ORCID: 0000-0002-3162-6386 AD - Chemistry Department, Faculty of Science, Alexandria University, Alexandria 21511, Egypt. FAU - Abdel-Aziz, Mohamed AU - Abdel-Aziz M AD - Department of Medicinal Chemistry, Faculty of Pharmacy, Minia University, Minia 61519, Egypt. AD - Department of Medicinal Chemistry, Faculty of Pharmacy, Minia National University, New Minia 61768, Egypt. LA - eng GR - 48085/Science and Technology Development Fund/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708434 OTO - NOTNLM OT - 1,2,3-triazoles OT - 1,3,5-triazines OT - AXL inhibitors OT - MCF-7 OT - click reactions OT - isocyanate synthesis OT - lapatinib OT - molecular docking OT - synergistic combination COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:45 PHST- 2026/06/28 00:00 [received] PHST- 2026/08/07 00:00 [revised] PHST- 2026/08/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - ph19101656 [pii] AID - pharmaceuticals-19-01656 [pii] AID - 10.3390/ph19101656 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 20;19(10):1656. doi: 10.3390/ph19101656. PMID- 42943349 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 19 TI - Design, Synthesis, and Evaluation of Novel Adenine-Based Hydrazones as Potential Anticancer Agents: Cytotoxicity Against A549 and MRC-5 Cell Lines with In Silico Insights. LID - 10.3390/ph19101652 [doi] LID - 1652 AB - Background/Objectives: Lung cancer remains a leading cause of global cancer-related mortality, necessitating the development of new therapeutic candidates. In this study, we aimed to design, synthesize, and biologically evaluate novel adenine-based hydrazone derivatives as potential anticancer candidates. Methods: A series of novel adenine-hydrazones were synthesized via the condensation of 2-(6-amino-9H-purin-9-yl)acetohydrazide with various aldehydes and isatin derivatives. Their antiproliferative activities against human lung adenocarcinoma (A549) and normal lung fibroblast (MRC-5) cell lines were evaluated using the MTT assay. Mechanistic investigations-including cell membrane damage (LDH release), oxidative stress (TOS, iROS), mitochondrial membrane potential (MMP, DeltaPsim), cell cycle distribution, and apoptosis (Annexin V/PI)-were conducted for the lead derivatives, complemented by multi-target in silico molecular docking against PLK1, TrxR1, and MDM2. Results: Compounds 3d, 3c, and 4j showed the strongest antiproliferative activity within the synthesized series against A549 cells, with IC(50) values of 37.2 +/- 1.8 microM, 41.8 +/- 1.9 microM, and 42.9 +/- 1.7 microM, respectively, together with comparatively more favorable A549/MRC-5 activity profiles than several other derivatives. These compounds induced oxidative stress-associated cellular injury, accompanied by mitochondrial depolarization, G2/M phase accumulation, and Annexin V-confirmed apoptotic cell death. Complementary multi-target in silico molecular docking provided theoretical insights into potential binding interactions with PLK1, TrxR1, and MDM2. Conclusions: Adenine-hydrazone hybrids, particularly 5-halogenated isatin derivatives such as compound 3d, provide preliminary lead scaffolds with moderate in vitro cytotoxicity against lung cancer cells, serving as a platform for future structural optimization to enhance potency and selectivity. FAU - Mentese, Emre AU - Mentese E AUID- ORCID: 0000-0002-3408-8202 AD - Department of Chemistry, Faculty of Art and Sciences, Recep Tayyip Erdogan University, 53100 Rize, Turkiye. FAU - Caliskan, Nedime AU - Caliskan N AD - Department of Chemistry, Faculty of Art and Sciences, Recep Tayyip Erdogan University, 53100 Rize, Turkiye. FAU - Emirik, Mustafa AU - Emirik M AUID- ORCID: 0000-0001-9489-9093 AD - Department of Chemistry, Faculty of Art and Sciences, Recep Tayyip Erdogan University, 53100 Rize, Turkiye. FAU - Guner, Adem AU - Guner A AUID- ORCID: 0000-0003-3295-3538 AD - Department of Medical Biology, Faculty of Medicine, Agri Ibrahim Cecen University, 04100 Agri, Turkiye. LA - eng GR - 020260080170585/Recep Tayyip Erdogan University Development Foundation/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708028 OTO - NOTNLM OT - A549 (lung cancer) OT - adenine OT - hydrazone OT - isatin OT - molecular docking COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:45 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ph19101652 [pii] AID - pharmaceuticals-19-01652 [pii] AID - 10.3390/ph19101652 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 19;19(10):1652. doi: 10.3390/ph19101652. PMID- 42943348 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 19 TI - A Pathway-Driven Computational Drug Discovery Framework Integrating Machine Learning and Virtual Screening for Identification of Anti-Rheumatoid Arthritis Natural Small Molecules. LID - 10.3390/ph19101651 [doi] LID - 1651 AB - Objective: This study aimed to establish a pathway-driven computational drug discovery framework integrating machine learning, target prioritization, virtual screening, molecular dynamics (MD) simulations, and in vitro evaluation to identify natural small molecules with potential anti-rheumatoid arthritis (RA) activity. Methods: Sixteen RA-related Gene Expression Omnibus (GEO) datasets were included, with 15 cohorts used for model development and one reserved for independent external validation. Pathway features quantified by gene set variation analysis (GSVA) were subjected to cohort-aware feature selection using a leave-one-dataset-out framework across seven machine-learning algorithms. Predictive modeling and SHapley Additive exPlanations (SHAP) analysis were subsequently performed, followed by random-effects meta-analysis (REMA) and network analysis for candidate-target prioritization. Natural compounds were screened by molecular docking and further evaluated using ligand efficiency, multiple scoring approaches, explicit-membrane MD simulations, and a TNF-alpha-induced MH7A inflammatory model. Results: Ten stable RA-associated pathways were identified. The final CAT+ET model achieved ROC-AUCs of 0.783 (95% CI, 0.731-0.836) and 0.610 (95% CI, 0.496-0.720) in the internal and independent external validation sets, respectively, indicating limited cross-cohort generalizability. SHAP and cross-cohort analyses supported Complement and coagulation cascades for downstream investigation, and integrated expression and network evidence prioritized C3AR1 as a candidate target. Virtual screening identified four candidate natural compounds (SM1-SM4). Ligand-size analysis, ligand efficiency, multiple scoring approaches, and explicit-membrane MD simulations provided complementary structural and energetic assessments of these candidates. Among them, SM1 showed comparatively favorable computational characteristics and a relatively consistent reduction in the inflammatory markers IL6 and MMP3 in TNF-alpha-stimulated MH7A cells, accompanied by changes in C3AR1 expression. These convergent computational and experimental findings supported the prioritization of SM1 for further investigation. Conclusions: This study established a pathway-driven framework linking cross-cohort pathway identification with candidate-target prioritization and natural-product screening in RA. Complement and coagulation cascades and C3AR1 were prioritized for downstream investigation, while integrated computational and experimental evidence identified SM1 as a candidate with promising anti-inflammatory potential. Although direct compound-C3AR1 engagement and its functional involvement require further validation, the present study provides a systematic and extensible strategy for translating robust RA-associated pathway signals into candidate targets and natural compounds, offering a valuable foundation for subsequent natural-product drug discovery in RA. FAU - Sun, Jijia AU - Sun J AUID- ORCID: 0000-0001-5068-4357 AD - Laboratory of Mathematical and Computational Intelligence, School of Artificial Intelligence, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. FAU - Wang, Yuezhen AU - Wang Y AUID- ORCID: 0009-0008-9313-592X AD - Laboratory of Mathematical and Computational Intelligence, School of Artificial Intelligence, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. FAU - Ye, Hong AU - Ye H AD - Laboratory of Mathematical and Computational Intelligence, School of Artificial Intelligence, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. FAU - Chen, Jialiang AU - Chen J AD - Center for Drug Safety Evaluation, Institute of Innovative Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. FAU - Chen, Yilin AU - Chen Y AUID- ORCID: 0009-0001-2639-5296 AD - Laboratory of Mathematical and Computational Intelligence, School of Artificial Intelligence, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. FAU - Yao, Guangtao AU - Yao G AD - Center for Drug Safety Evaluation, Institute of Innovative Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. LA - eng GR - 24KFL027/Shanghai University of Traditional Chinese Medicine/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708292 OTO - NOTNLM OT - C3AR1 OT - ensemble learning OT - molecular dynamics OT - natural products OT - pathway prioritization OT - rheumatoid arthritis OT - virtual screening COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:45 PHST- 2026/07/30 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ph19101651 [pii] AID - pharmaceuticals-19-01651 [pii] AID - 10.3390/ph19101651 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 19;19(10):1651. doi: 10.3390/ph19101651. PMID- 42943347 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 19 TI - Cryptococcosis After Initiation of Janus Kinase Inhibitors or Biologic DMARDs in Rheumatoid Arthritis: Three Pairwise Active-Comparator Analyses of Propensity Score-Matched TriNetX Cohorts, 2005-2025. LID - 10.3390/ph19101650 [doi] LID - 1650 AB - Background/Objectives: Comparative safety evidence for cryptococcosis during advanced rheumatoid arthritis (RA) therapy is limited. We compared newly recorded cryptococcosis after initiation of Janus kinase inhibitors (JAKi), tumor necrosis factor (TNF) inhibitors (TNFi), and non-TNF biologic disease-modifying antirheumatic drugs (non-TNF bDMARDs). Methods: Three retrospective active-comparator analyses were conducted in the TriNetX Global Collaborative Network: JAKi versus TNFi, JAKi versus non-TNF bDMARDs, and TNFi versus non-TNF bDMARDs. After 1:1 propensity score matching, outcomes from day 1 to 10 years after initiation, capped at 30 June 2025, were analyzed using Cox proportional hazards models. E-values assessed sensitivity to unmeasured confounding. Results: Matched cohorts included 27,833, 32,440, and 37,351 patients per group, respectively; all absolute standardized mean differences were <0.10. Events occurred in 20 JAKi and 12 TNFi initiators (hazard ratio [HR], 2.20; 95% confidence interval [CI], 1.06-4.54) and in 22 JAKi and 13 non-TNF bDMARD initiators (HR, 2.15; 95% CI, 1.07-4.31). In the secondary comparison, 17 TNFi and 19 non-TNF bDMARD initiators had events (HR, 0.83; 95% CI, 0.43-1.60). Point-estimate E-values for the JAKi comparisons were 3.82 and 3.72; CI-limit E-values were 1.31 and 1.34. Conclusions: JAKi initiation was associated with an approximately twofold higher hazard of newly recorded cryptococcosis than either biologic comparator, while estimated 10-year cumulative event probabilities were below 0.2% in all groups. The separately selected and matched populations do not support an indirect ranking of treatment classes. Low event counts, wide CIs, possible outcome misclassification, fixed exposure classification, and residual confounding preclude causal conclusions. The findings require independent confirmation. FAU - Tay, Ying Xiu AU - Tay YX AD - Division of Allergy, Immunology and Rheumatology, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Dalin 622401, Chiayi, Taiwan. FAU - He, Yi-Ting AU - He YT AD - Center for Health Data & Advanced Translational Analytics, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Dalin 622401, Chiayi, Taiwan. AD - Department of Public Health, College of Medicine, National Cheng Kung University, Tainan City 704302, Taiwan. FAU - Koo, Malcolm AU - Koo M AUID- ORCID: 0000-0002-9242-9167 AD - Department of Medical Research, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Dalin 622401, Chiayi, Taiwan. AD - Dalla Lana School of Public Health, University of Toronto, Toronto, ON M5T 3M7, Canada. FAU - Lu, Ming-Chi AU - Lu MC AUID- ORCID: 0000-0001-9051-0351 AD - Division of Allergy, Immunology and Rheumatology, Dalin Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Dalin 622401, Chiayi, Taiwan. AD - School of Medicine, Tzu Chi University, Hualien City 970374, Hualien, Taiwan. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708528 OTO - NOTNLM OT - Janus kinase inhibitors OT - TriNetX OT - cryptococcosis OT - opportunistic infection OT - pharmacovigilance OT - post-authorization safety OT - real-world evidence OT - rheumatoid arthritis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:45 PHST- 2026/09/13 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ph19101650 [pii] AID - pharmaceuticals-19-01650 [pii] AID - 10.3390/ph19101650 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 19;19(10):1650. doi: 10.3390/ph19101650. PMID- 42943342 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 19 TI - Synthesis, Biological Evaluation, and Molecular Dynamics Studies of Novel 1,2,4-Triazole-Functionalized Benzene-Sulfonate Derivatives as Potential Enzyme Inhibitors and Cytotoxicity in HepG2 Cells. LID - 10.3390/ph19101645 [doi] LID - 1645 AB - Background/Objectives: In this study, novel benzenesulfonate derivatives containing a 1,2,4-triazole ring were synthesized. Methods: Their molecular structures were characterized by IR, (1)H NMR, and (13)C NMR spectroscopy, together with Q-TOF LC/MS analyses. For compounds 5b and 5d, the available mass spectrometric data were not used for structural confirmation, and their structural assignments were based primarily on the spectroscopic data. The inhibitory effects of the synthesized compounds (3-5) against acetylcholinesterase (AChE) and glutathione S-transferase (GST) enzymes were evaluated. Results: Comparison of the results with the reference inhibitors, donepezil and ethacrynic acid, showed that compound 3e exhibited the highest AChE inhibitory activity among the tested compounds, with an IC(50) value of 1.60 +/- 0.198 muM. In addition, compound 4a showed remarkable GST inhibitory activity, with an IC(50) value of 1.95 +/- 0.492 muM, demonstrating greater potency than the reference inhibitor. The cytotoxic effects of the selected compounds were further assessed against the HepG2 cell line after 24 and 48 h of treatment. Overall, the synthesized molecules exhibited potent enzyme inhibitory activities together with promising cytotoxic effects, highlighting their potential as lead candidates for further biological investigations. In silico analyses were performed to elucidate the molecular basis of the experimentally observed acetylcholinesterase (AChE) inhibitory activity of the synthesized derivatives. Molecular docking against human AChE (PDB ID: 4EY7) identified compound 3e as the most favorable binder among the synthesized series, while 3f also exhibited a strong binding profile. Subsequent 100 ns molecular dynamics simulations of the hAChE-3e, hAChE-3f, and hAChE-donepezil complexes demonstrated stable protein conformations and persistent ligand accommodation within the catalytic gorge. MM/GBSA calculations further indicated a more favorable binding-energy profile for 3e than for 3f, whereas donepezil showed the most favorable overall calculated binding energy. Per-residue decomposition highlighted key gorge residues, including Trp86, Trp286, Tyr337, Tyr341, Phe295, and Phe297, as important contributors to ligand stabilization. Conclusions: Collectively, these computational findings provide structural and energetic support for the experimentally observed AChE inhibitory activity of the lead derivatives. FAU - Unver, Yasemin AU - Unver Y AD - Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, 61080 Trabzon, Turkiye. FAU - Turkan, Fikret AU - Turkan F AD - Department of Basic Sciences, Faculty of Dentistry, Igdir University, 76000 Igdir, Turkiye. AD - Department of Basic Medical Science, Medical of Faculty, Nakhchivan State University, AZ 7012 Nakhchivan, Azerbaijan. FAU - Guler, Halil Ibrahim AU - Guler HI AUID- ORCID: 0000-0002-7261-6790 AD - Department of Molecular Biology and Genetics, Faculty of Science, Karadeniz Technical University, 61080 Trabzon, Turkiye. FAU - Oguz, Ercan AU - Oguz E AD - Department of Medical Services and Techniques, Health Services Vocational School, Igdir University, 76000 Igdir, Turkiye. FAU - Usta, Tugba AU - Usta T AD - Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, 61080 Trabzon, Turkiye. AD - Department of Chemistry, Graduate School of Natural and Applied Science, Karadeniz Technical University, 61080 Trabzon, Turkiye. FAU - Celik, Fatih AU - Celik F AD - Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, 61080 Trabzon, Turkiye. LA - eng GR - FBA-2025-16757/Karadeniz Technical University/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708584 OTO - NOTNLM OT - AChE OT - GST OT - cytotoxicity study OT - enzyme inhibition OT - molecular docking OT - molecular dynamics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:45 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ph19101645 [pii] AID - pharmaceuticals-19-01645 [pii] AID - 10.3390/ph19101645 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 19;19(10):1645. doi: 10.3390/ph19101645. PMID- 42943341 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 19 TI - Quality of Life in Patients with Treatment-Resistant Depression Treated with Intranasal Esketamine as Add-On Therapy to an SSRI-A Prospective, Comparative Study in Real-World Clinical Practice. LID - 10.3390/ph19101644 [doi] LID - 1644 AB - Treatment-resistant depression (TRD) remains one of the most clinically challenging forms of depressive disorders. In many patients, reduction in symptom severity does not translate into full functional recovery; therefore, assessment of treatment effectiveness should also include health-related quality of life (HRQoL). Objective: The aim of the study was to compare changes in HRQoL in patients with TRD receiving a treatment strategy including intranasal esketamine as add-on therapy to ongoing treatment based on selective serotonin reuptake inhibitors (SSRIs) and in patients treated with SSRI-based therapy without esketamine. Materials and Methods: A prospective, observational, comparative cohort study was conducted in real-world clinical practice. Sixty adult patients were included: 30 received intranasal esketamine as add-on therapy to ongoing SSRI-based treatment and 30 received SSRI-based treatment without esketamine. The study protocol did not impose a separate SSRI dose-optimization algorithm; drug selection and dose modifications remained at the discretion of the treating physician in accordance with routine clinical practice. Quality of life was assessed using the World Health Organization Quality of Life-BREF (WHOQOL-BREF) and the 36-Item Short Form Health Survey (SF-36). Depressive symptom severity was assessed using the Montgomery-Asberg Depression Rating Scale (MADRS) and the Hamilton Depression Rating Scale (HDRS). Assessments were performed at baseline, weeks 4, 8, and 12, and after 12 months. Results: Both groups showed improvement in quality of life and reduction in depressive symptom severity, with larger absolute changes in the group receiving esketamine. Differences involved WHOQOL-BREF domains and selected standard SF-36 subscales. Conclusions: In this observational cohort, the treatment strategy including intranasal esketamine was associated with greater improvement in several HRQoL measures than SSRI-based treatment without esketamine. Because treatment exposure was not assigned by the investigators and residual confounding cannot be excluded, the findings should not be interpreted as evidence of an isolated causal effect of esketamine. FAU - Szawarnoga, Daniel AU - Szawarnoga D AUID- ORCID: 0009-0003-5330-0007 AD - Department of Psychiatry, Department of Neurology, Faculty of Health Sciences in Katowice, Medical University of Silesia in Katowice, 40-752 Katowice, Poland. FAU - Fojcik, Joanna AU - Fojcik J AD - Department of Psychiatry, Department of Neurology, Faculty of Health Sciences in Katowice, Medical University of Silesia in Katowice, 40-752 Katowice, Poland. FAU - Gorski, Michal AU - Gorski M AUID- ORCID: 0000-0002-0654-3765 AD - Department of Psychiatry, Department of Neurology, Faculty of Health Sciences in Katowice, Medical University of Silesia in Katowice, 40-752 Katowice, Poland. FAU - Krzystanek, Marek AU - Krzystanek M AUID- ORCID: 0000-0002-1665-7344 AD - Department of Clinical and Community Psychiatry and Psychology, Collegium Medicum, WSB University, 41-300 Dabrowa Gornicza, Poland. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708465 OTO - NOTNLM OT - SF-36 OT - WHOQOL-BREF OT - antidepressant treatment OT - esketamine OT - patient-reported outcomes OT - quality of life OT - real-world evidence OT - treatment-resistant depression COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:45 PHST- 2026/09/13 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ph19101644 [pii] AID - pharmaceuticals-19-01644 [pii] AID - 10.3390/ph19101644 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 19;19(10):1644. doi: 10.3390/ph19101644. PMID- 42943338 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 18 TI - Dosimetry Data Infrastructure for Individualized Radiopharmaceutical Therapy: International Experience and a Proposed Framework for China. LID - 10.3390/ph19101641 [doi] LID - 1641 AB - Background/Objectives: There is increasing interest in moving selected radiopharmaceutical therapy (RPT) applications toward treatment informed by patient-specific quantitative data. Patient-specific dosimetry supports this transition, but routine implementation remains limited by methodological heterogeneity and incomplete linkage of absorbed dose with response, toxicity, and subsequent treatment decisions. This review examines evidence relevant to longitudinal dosimetry infrastructure and proposes a framework for China. Methods: This narrative review mapped evidence from established therapies, including (177)Lu-DOTATATE, (177)Lu-PSMA-617, (131)I-NaI, and (131)I-meta-iodobenzylguanidine ((131)I-MIBG), with (90)Y microsphere selective internal radiation therapy used as a methodologically distinct comparator. International registries, quantitative imaging standards, and dosimetry resources were reviewed in parallel. Results: Available studies show substantial interpatient variation in absorbed dose and growing evidence of dose-response relationships. However, prescription, dosimetry methods, outcome definitions, and follow-up remain heterogeneous. Existing platforms address separate components of RPT care, but few link administration, pretherapy imaging, post-therapy quantitative imaging, dosimetry, clinical outcomes, toxicity, and later decisions across a complete treatment course. We propose seven core data domains and a three-tier architecture comprising hospital-based treatment decision platforms, regional dosimetry centers, and a national data center. Conclusions: A standardized longitudinal dosimetry infrastructure could improve multicenter comparability and provide a testable data foundation for individualized treatment, radiopharmaceutical development, and real-world evaluation. The proposed architecture is conceptual and requires staged consensus, pilot implementation, and prospective validation. FAU - Huang, Qingqing AU - Huang Q AD - Shanghai Key Laboratory of Molecular Imaging, Pudong Gongli Hospital, Shanghai University of Medicine and Health Sciences, Shanghai 201318, China. FAU - Huang, Gang AU - Huang G AD - Shanghai Key Laboratory of Molecular Imaging, Pudong Gongli Hospital, Shanghai University of Medicine and Health Sciences, Shanghai 201318, China. AD - Hangzhou Institute of Medicine, Chinese Academy of Sciences, Hangzhou 310018, China. LA - eng GR - XDA0580000/the Strategic Priority Research Program of Chinese Academy of Sciences/ GR - 18DZ2260400/Shanghai Key Laboratory of Molecular Imaging/ PT - Journal Article PT - Review DEP - 20261018 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708463 OTO - NOTNLM OT - dosimetry database OT - individualized dosimetry OT - quantitative imaging OT - radiopharmaceutical therapy OT - real-world evidence OT - standardization OT - treatment decision support COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:45 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - ph19101641 [pii] AID - pharmaceuticals-19-01641 [pii] AID - 10.3390/ph19101641 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 18;19(10):1641. doi: 10.3390/ph19101641. PMID- 42943334 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 16 TI - Identification of Novel Potential Tubulin-Targeting Heterocyclic Compounds via Integrated Virtual Screening and In Vitro Studies. LID - 10.3390/ph19101637 [doi] LID - 1637 AB - Background: The development of novel tubulin-targeting agents remains a promising strategy in anticancer drug design. In the present study, novel heterocyclic tubulin-targeting compounds were identified using a complex in silico/in vitro workflow. Methods: An in-house library of approximately 8000 structurally diverse heterocyclic compounds was screened for antiproliferative activity, yielding 260 experimentally validated hits. These compounds were docked against the colchicine, vinca, and taxane binding sites of tubulin. Selected compounds were also tested for their effects on cellular beta-tubulin protein levels in Jurkat cells. Results: Docking analysis indicated that 42 compounds matched or exceeded colchicine's binding energy, 3 compounds were comparable to vinblastine, and 18 compounds surpassed paclitaxel, suggesting the colchicine and taxane sites as preferred targets. Five top-scoring compounds (Les-6370, Les-4272, Les-4309, Les-4403, and Les-1419) were further analyzed via molecular dynamics simulations, confirming stable binding-particularly at the colchicine site-and favorable hydrogen-bonding and lipophilic interactions. At 10 microM, Les-1419 and Les-4309 reduced total cellular beta-tubulin protein levels in Jurkat cells by 57.73% and 54.31%, respectively, relative to the effect observed for the reference compound Combretastatin A-4. Conclusions: This study resulted in the identification of novel starting scaffolds with favorable tubulin interaction activity and predicted drug-like properties, representing structurally novel parent structures and serving as promising starting points for further structural optimization and analogue development. FAU - Deyak, Yaroslava AU - Deyak Y AUID- ORCID: 0000-0002-2733-2297 AD - Department of Pharmaceutical, Organic and Bioorganic Chemistry, Danylo Halytsky Lviv National Medical University, Pekarska 69, 79010 Lviv, Ukraine. AD - Department of Pharmaceutical Disciplines, Uzhhorod National University, Narodna Square 3, 88000 Uzhhorod, Ukraine. FAU - Khylyuk, Dmytro AU - Khylyuk D AUID- ORCID: 0000-0001-9306-7463 AD - Chair and Department of Organic Chemistry, Medical University of Lublin, Chodzki 4a, 20-093 Lublin, Poland. FAU - Dudchak, Rostyslav AU - Dudchak R AD - Department of Synthesis and Technology of Drugs, Medical University of Bialystok, Kilinskiego 1, 15-089 Bialystok, Poland. FAU - Finiuk, Nataliya AU - Finiuk N AUID- ORCID: 0000-0002-4112-8815 AD - Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology of National Academy of Sciences of Ukraine, Drahomanov14/16, 79005 Lviv, Ukraine. AD - Molecular Design Center, Danylo Halytsky Lviv National Medical University, Pekarska 69, 79010 Lviv, Ukraine. FAU - Ivasechko, Iryna AU - Ivasechko I AD - Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology of National Academy of Sciences of Ukraine, Drahomanov14/16, 79005 Lviv, Ukraine. AD - Molecular Design Center, Danylo Halytsky Lviv National Medical University, Pekarska 69, 79010 Lviv, Ukraine. FAU - Kozak, Yuliia AU - Kozak Y AUID- ORCID: 0000-0002-6793-0662 AD - Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell Biology of National Academy of Sciences of Ukraine, Drahomanov14/16, 79005 Lviv, Ukraine. AD - Molecular Design Center, Danylo Halytsky Lviv National Medical University, Pekarska 69, 79010 Lviv, Ukraine. FAU - Solodiak, Nazar AU - Solodiak N AD - Department of Pharmaceutical, Organic and Bioorganic Chemistry, Danylo Halytsky Lviv National Medical University, Pekarska 69, 79010 Lviv, Ukraine. AD - Molecular Design Center, Danylo Halytsky Lviv National Medical University, Pekarska 69, 79010 Lviv, Ukraine. FAU - Derkach, Halyna AU - Derkach H AUID- ORCID: 0000-0002-0051-6950 AD - Department of Chemistry, Pharmaceutical Analysis and Postgraduate Education, Ivano-Frankivsk National Medical University Halytska St., 124K, 76008 Ivano-Frankivsk, Ukraine. FAU - Pietruszynska, Marta AU - Pietruszynska M AD - Department of Clinical Pharmacology, Medical University of Bialystok, Kilinskiego 1, 15-089 Bialystok, Poland. FAU - Podolak, Magdalena AU - Podolak M AD - Department of Biotechnology, Medical University of Bialystok, Kilinskiego 1, 15-089 Bialystok, Poland. FAU - Gornowicz, Agnieszka AU - Gornowicz A AUID- ORCID: 0000-0002-0945-7870 AD - Department of Biotechnology, Medical University of Bialystok, Kilinskiego 1, 15-089 Bialystok, Poland. FAU - Bielawski, Krzysztof AU - Bielawski K AD - Department of Synthesis and Technology of Drugs, Medical University of Bialystok, Kilinskiego 1, 15-089 Bialystok, Poland. FAU - Bielawska, Anna AU - Bielawska A AUID- ORCID: 0000-0001-9164-8440 AD - Department of Biotechnology, Medical University of Bialystok, Kilinskiego 1, 15-089 Bialystok, Poland. FAU - Holota, Serhii AU - Holota S AUID- ORCID: 0000-0002-9892-437X AD - Department of Pharmaceutical, Organic and Bioorganic Chemistry, Danylo Halytsky Lviv National Medical University, Pekarska 69, 79010 Lviv, Ukraine. AD - Molecular Design Center, Danylo Halytsky Lviv National Medical University, Pekarska 69, 79010 Lviv, Ukraine. FAU - Lesyk, Roman AU - Lesyk R AUID- ORCID: 0000-0002-3322-0080 AD - Department of Pharmaceutical, Organic and Bioorganic Chemistry, Danylo Halytsky Lviv National Medical University, Pekarska 69, 79010 Lviv, Ukraine. AD - Molecular Design Center, Danylo Halytsky Lviv National Medical University, Pekarska 69, 79010 Lviv, Ukraine. AD - Department of Biotechnology and Cell Biology, University of Information Technology and Management in Rzeszow, St. Sucharskiego 2, 35-225 Rzeszow, Poland. LA - eng GR - 2023.05/0021./National Research Foundation of Ukraine/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708617 OTO - NOTNLM OT - anticancer agents OT - diarylpyrazoline OT - heterocyclic compounds OT - molecular docking OT - molecular dynamics OT - tubulin inhibitors COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:45 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ph19101637 [pii] AID - pharmaceuticals-19-01637 [pii] AID - 10.3390/ph19101637 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 16;19(10):1637. doi: 10.3390/ph19101637. PMID- 42943328 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 14 TI - Green Synthesis of Silver Nanoparticles Using Artemisia absinthium and Ziziphus vulgaris Extracts: Comparative Evaluation of Antibacterial, Anti-Inflammatory, Wound-Healing and Anticancer Activities. LID - 10.3390/ph19101630 [doi] LID - 1630 AB - Background: Plant-mediated synthesis of silver nanoparticles (AgNPs) has emerged as a sustainable alternative to conventional chemical methods, offering environmentally friendly routes for producing nanomaterials with biomedical potential. This study employed aqueous extracts of Artemisia absinthium and Ziziphus vulgaris to synthesize AgNPs and evaluate their antibacterial, anti-inflammatory, anticancer, wound healing activities. Methods: Silver nanoparticles were synthesized by mixing aqueous plant extracts with 1 mM silver nitrate under ambient conditions. The formation of AgNPs was confirmed by UV-Visible spectroscopy, while morphology and structure were characterized using Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), and Fourier-transform infrared (FTIR) spectroscopy. Moreover, the biosynthesized silver nanoparticles were further characterized by dynamic light scattering (DLS) and zeta potential measurements to determine hydrodynamic size and surface charge, and by X-ray diffraction (XRD) to confirm crystalline phase. Energy-dispersive X-ray spectroscopy (EDX) was also performed to assess elemental composition, providing a comprehensive physicochemical profile of the plant-mediated AgNPs. Antibacterial activity was tested against Escherichia coli and Staphylococcus aureus using the agar well diffusion, minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) methods. Anti-inflammatory activity was evaluated by hypotonicity-induced human red blood cell (HRBC) membrane stabilization assay. Cytotoxic activity was evaluated using the MTT assay against a panel of human cell lines comprising MCF-7 breast cancer cells, A549 lung cancer cells, HepG2 hepatic cancer cells, and WI-38 non-cancerous lung fibroblasts to assess both anticancer potency and selectivity toward normal cells. Scratch wound healing was also evaluated. Results: The biosynthesized AgNPs showed surface plasmon resonance bands at 420-450 nm and mainly spherical particles with sizes of 15-22 nm. FTIR analysis indicated hydroxyl and carbonyl groups involved in nanoparticle reduction and stabilization. DLS and zeta potential analyses revealed monomodal size distributions and strongly negative surface charges (approximately -33 to -34 mV), indicating homogeneous, electrostatically stabilized colloids. XRD patterns confirmed a face-centered cubic metallic silver phase with a dominant (111) orientation, while EDX spectra verified the presence of elemental silver alongside signals from the plant-derived capping layer. The nanoparticles displayed remarkable antibacterial activity against both bacteria, strong inhibition of erythrocyte hemolysis, and dose-dependent cytotoxicity toward MCF-7 cells with IC(50) values of 19-21 microg mL(-1). MIC values ranged from 15.62 to 125 microg mL(-1), with the lowest MIC observed for Ziziphus stem-mediated AgNPs (15.62 microg mL(-1)). All AgNPs samples exhibited enhanced wound healing, with closure percentages reaching up to 93.4% in Ziziphus leaves compared to WI-38 control (77.6%). Good colloidal stability was maintained for 90 days. Conclusions: These findings show that Artemisia absinthium and Ziziphus vulgaris are effective sources for green synthesis of stable AgNPs with multifunctional in vitro biological activities. This pro-healing effect is consistent with their demonstrated antimicrobial activity, supporting their dual functionality as both antibacterial and wound-healing materials. However, the biological and therapeutic activities require further in vivo confirmation. The results support their biomedical and environmentally sustainable nanotechnology applications. FAU - Gamal, Mohammed AU - Gamal M AUID- ORCID: 0000-0002-4674-6516 AD - Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University, Alshaheed Shehata Ahmed Hegazy St., Beni-Suef 62574, Egypt. FAU - Omer, Asma B AU - Omer AB AUID- ORCID: 0000-0003-0351-3576 AD - Department of Health Science, College of Health and Rehabilitation Sciences, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia. FAU - Saleh, Asmaa AU - Saleh A AUID- ORCID: 0000-0002-6286-319X AD - Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia. FAU - Bayram, Roula AU - Bayram R AUID- ORCID: 0000-0002-8193-5291 AD - Pharmaceutical Science, Pharmacy Program, Batterjee Medical College, Jeddah 21442, Saudi Arabia. AD - Department of Quality Control and Pharmaceutical Chemistry, Faculty of Pharmacy, University of Aleppo, Aleppo P.O. Box 12212, Syria. FAU - Naguib, Ibrahim A AU - Naguib IA AUID- ORCID: 0000-0002-5923-1466 AD - Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, Taif 21944, Saudi Arabia. FAU - Elsayed, Mohamed Ahmed AU - Elsayed MA AUID- ORCID: 0000-0003-2673-3301 AD - Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Fayoum University, Fayoum 63514, Egypt. FAU - Mousa, Safa AU - Mousa S AD - Chemistry Department, Faculty of Science, AlQuba Campus, Derna University, Derna P.O. Box 125, Libya. FAU - Hasan, Hamad M Adress AU - Hasan HMA AD - Chemistry Department, Faculty of Science, Omar Al-Mukhtar University, Bayda P.O. Box 991, Libya. LA - eng GR - PNURSP2026R854/Princess Nourah bint Abdulrahman University/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708049 OTO - NOTNLM OT - Artemisia absinthium OT - Ziziphus vulgaris OT - anti-inflammatory activity OT - antibacterial activity OT - cytotoxicity OT - green synthesis OT - silver nanoparticles OT - wound-healing COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:45 PHST- 2026/09/16 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - ph19101630 [pii] AID - pharmaceuticals-19-01630 [pii] AID - 10.3390/ph19101630 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 14;19(10):1630. doi: 10.3390/ph19101630. PMID- 42943326 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 14 TI - The MASLD-Heart Axis: Biological Mechanisms, Cardiac Remodeling, and Clinical Implications. LID - 10.3390/ph19101629 [doi] LID - 1629 AB - Background/Objectives: Metabolic dysfunction-associated steatotic liver disease (MASLD), the hepatic manifestation of cardiometabolic dysfunction, is closely linked to atherosclerosis, heart failure, and adverse cardiovascular outcomes. This review summarizes current evidence on the biological basis of the MASLD-heart axis, with particular emphasis on vascular injury and adverse cardiac remodeling. Methods: A narrative review of contemporary literature was conducted, including experimental studies, observational cohorts, meta-analyses, randomized clinical trials, and current guideline recommendations addressing the cardiohepatic axis. Results: The interaction between the liver and the heart involves interconnected mechanisms, including insulin resistance, lipotoxicity, oxidative stress, chronic inflammation, endothelial dysfunction, and neurohormonal activation. Experimental and translational evidence supports biologically plausible links between MASLD-related metabolic and inflammatory pathways and vascular or myocardial injury, while clinical studies consistently associate MASLD with arterial stiffness, microvascular dysfunction, atherosclerotic disease, adverse cardiac remodeling, heart failure, atrial fibrillation, and cardiovascular events. Several of these associations persist after adjustment for conventional cardiometabolic risk factors, although residual confounding and shared risk determinants limit causal inference. Non-invasive tools, including the Fibrosis-4 (FIB-4) score and transient elastography, may help identify patients at higher risk of advanced fibrosis and cardiometabolic complications. Lifestyle interventions remain the cornerstone of management, while glucagon-like peptide-1 receptor agonists, sodium-glucose cotransporter-2 inhibitors, and fibroblast growth factor-21 analogs may provide combined hepatic and cardiovascular benefits. Conclusions: The cardiohepatic axis is an important component of cardiometabolic disease, linking hepatic dysfunction with vascular and myocardial injury through shared biological pathways. Incorporating hepatic assessment into cardiovascular risk evaluation may support earlier identification of high-risk patients. FAU - Oikonomou, Evangelos AU - Oikonomou E AUID- ORCID: 0000-0001-8079-0599 AD - 3rd Cardiology Department, "Sotiria" General Hospital of Chest Diseases of Athens, Athina Ioanni Martinou Endowed Chair; Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Machairas, Nikolaos P AU - Machairas NP AUID- ORCID: 0009-0004-6398-8156 AD - First Department of Cardiology, School of Medicine, National and Kapodistrian University of Athens, Hippokration General Hospital, 11527 Athens, Greece. FAU - Theofilis, Panagiotis AU - Theofilis P AUID- ORCID: 0000-0001-9260-6306 AD - First Department of Cardiology, School of Medicine, National and Kapodistrian University of Athens, Hippokration General Hospital, 11527 Athens, Greece. FAU - Louka, Anna Maria AU - Louka AM AD - Third Department of Cardiology, "Sotiria" Chest Diseases General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Xenou, Maria AU - Xenou M AD - Third Department of Cardiology, "Sotiria" Chest Diseases General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Stampouloglou, Panagiota K AU - Stampouloglou PK AD - Third Department of Cardiology, "Sotiria" Chest Diseases General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Katsarou, Ourania AU - Katsarou O AD - Third Department of Cardiology, "Sotiria" Chest Diseases General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Kokkinos, Nikolaos K AU - Kokkinos NK AD - Third Department of Cardiology, "Sotiria" Chest Diseases General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Lysandrou, Antonios AU - Lysandrou A AD - Third Department of Cardiology, "Sotiria" Chest Diseases General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Karamesinis-Spinthakis, Stergios AU - Karamesinis-Spinthakis S AUID- ORCID: 0009-0004-6221-428X AD - Third Department of Cardiology, "Sotiria" Chest Diseases General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Tousoulis, Dimitris AU - Tousoulis D AD - First Department of Cardiology, School of Medicine, National and Kapodistrian University of Athens, Hippokration General Hospital, 11527 Athens, Greece. FAU - Siasos, Gerasimos AU - Siasos G AD - Third Department of Cardiology, "Sotiria" Chest Diseases General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708144 OTO - NOTNLM OT - atherosclerosis OT - cardiovascular disease OT - endothelial dysfunction OT - heart failure OT - metabolic dysfunction-associated steatotic liver disease OT - ventricular remodeling COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:45 PHST- 2026/07/21 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - ph19101629 [pii] AID - pharmaceuticals-19-01629 [pii] AID - 10.3390/ph19101629 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 14;19(10):1629. doi: 10.3390/ph19101629. PMID- 42943325 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 14 TI - Targeting PAK1 Within Group I PAKs: Current Challenges and Pharmacological Perspectives. LID - 10.3390/ph19101628 [doi] LID - 1628 AB - The p21-activated kinase 1 (PAK1) has been investigated as a therapeutic target for more than two decades, particularly in cancer, where it contributes to cytoskeletal regulation, proliferation, survival, migration and therapy resistance. Despite the development of numerous potent inhibitors, selective pharmacological modulation of PAK1 remains difficult because of the high similarity of the group I PAK catalytic domains, especially PAK1 and PAK2, together with differences in conformational state, cellular localization and kinase-independent functions. This review examines PAK1 within the broader PAK kinase family and critically evaluates the principal pharmacological strategies developed to modulate its activity, including ATP-competitive and allosteric inhibition, targeted degradation, polypharmacology, combination therapies and indirect approaches. Recent structural studies indicate that local conformational differences between PAK1 and PAK2 can be exploited for improved isoform discrimination, while PAK1-selective degraders provide an alternative means of affecting both catalytic and non-catalytic functions. A snapshot analysis of current ChEMBL bioactivity data further shows that, although numerous compounds display high reported potency against PAK1, matched quantitative information for PAK2 and PAK3 is available for only a limited subset, leaving isoform selectivity unresolved for most compounds. Recent findings also position PAK1 within adaptive and treatment-resistance signaling rather than as a universal oncogenic dependency. Future progress will therefore depend not only on improving compound potency, but on integrating isoform profiling, cellular target engagement, structural information and disease-specific biomarkers to identify the settings in which PAK1 modulation can provide a meaningful therapeutic advantage. FAU - Ion, George Nicolae Daniel AU - Ion GND AUID- ORCID: 0000-0003-3835-9169 AD - Faculty of Pharmacy, "Carol Davila" University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania. FAU - Duta-Bratu, Cosmina Gabriela AU - Duta-Bratu CG AD - Faculty of Pharmacy, "Carol Davila" University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania. FAU - Mihai, Dragos Paul AU - Mihai DP AUID- ORCID: 0000-0003-2880-9381 AD - Faculty of Pharmacy, "Carol Davila" University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania. FAU - Nitulescu, George Mihai AU - Nitulescu GM AUID- ORCID: 0000-0002-2978-8052 AD - Faculty of Pharmacy, "Carol Davila" University of Medicine and Pharmacy, Traian Vuia 6, 020956 Bucharest, Romania. LA - eng GR - 28561/02.10.2023/Carol Davila University of Medicine and Pharmacy/ PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708658 OTO - NOTNLM OT - PAK1 OT - PROTAC OT - allosteric inhibition OT - cancer therapy OT - drug resistance OT - isoform selectivity OT - kinase inhibitors OT - kinase pharmacology OT - p21-activated kinase OT - targeted protein degradation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:45 PHST- 2026/09/07 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:45 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - ph19101628 [pii] AID - pharmaceuticals-19-01628 [pii] AID - 10.3390/ph19101628 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 14;19(10):1628. doi: 10.3390/ph19101628. PMID- 42943316 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 12 TI - Dexamethasone-Induced Insulin Resistance and Hepatic Metabolic Dysfunction: Modulatory Effects of Montelukast on SGK1-Associated Signaling and NLRP3/Leukotriene-Related Responses. LID - 10.3390/ph19101619 [doi] LID - 1619 AB - Background: Glucocorticoid-induced metabolic dysfunction and insulin resistance-related abnormalities are important complications associated with dexamethasone (DEX) exposure. This study investigated the protective effects of montelukast (MNT) against DEX-induced metabolic and hepatic disturbances and characterized associated oxidative, inflammatory, leukotriene-related, and insulin-signaling changes in rats. Methods: Male Wistar rats were allocated to five groups receiving vehicle, MNT (20 mg/kg), DEX (8 mg/kg), or DEX combined with MNT (10 or 20 mg/kg). Glycemic parameters, oral glucose tolerance, lipid profile, liver function biomarkers, oxidative stress indices, inflammatory mediators, leukotriene-related markers, and hepatic histopathological and ultrastructural changes were assessed. Selected insulin-signaling and metabolic markers were evaluated using immunohistochemistry, ELISA-based assays, and Western blotting. Results: DEX administration induced hyperglycemia, hyperinsulinemia, impaired glucose tolerance, increased HOMA2-IR, dyslipidemia, hepatic dysfunction, and histopathological and ultrastructural alterations. These abnormalities were accompanied by oxidative stress and changes in selected molecular markers, including SGK1, AKT, JNK, IRS1, GLUT4, PI3K/FOXO3/PPARgamma, GS/PEPCK/GSK, NF-kappaB/IL-6, NLRP3 abundance, and leukotriene-related markers, including LTD4 and CysLT2, together with alterations in phosphorylation-associated AMPK, PI3K, and ERK1/2 signaling markers. MNT attenuated several DEX-associated metabolic, oxidative, inflammatory, and hepatic abnormalities and modulated these molecular markers. Conclusions: MNT attenuated DEX-associated metabolic and hepatic disturbances in rats, accompanied by modulation of selected SGK1-associated insulin-signaling, metabolic, oxidative, inflammatory, and leukotriene-related markers. These findings support further investigation of MNT in experimental glucocorticoid-associated metabolic dysfunction. However, the molecular findings represent associated alterations rather than definitive evidence of direct pathway activation, target engagement, or causal mechanisms. FAU - Alresheedi, Ahmad A AU - Alresheedi AA AD - Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, ElGomhoria Street, Mansoura 35516, Egypt. FAU - Nour, Omnia A AU - Nour OA AD - Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, ElGomhoria Street, Mansoura 35516, Egypt. FAU - El-Kashef, Dalia H AU - El-Kashef DH AUID- ORCID: 0000-0002-0021-5533 AD - Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, ElGomhoria Street, Mansoura 35516, Egypt. FAU - Nader, Manar A AU - Nader MA AUID- ORCID: 0000-0002-8590-2622 AD - Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, ElGomhoria Street, Mansoura 35516, Egypt. AD - Faculty of Health Science Technology, Mansoura National University, Gamasa 7731168, Egypt. LA - eng PT - Journal Article DEP - 20261012 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708129 OTO - NOTNLM OT - AMPK/mTOR signaling OT - NLRP3 inflammasome OT - SGK1 OT - dexamethasone OT - hepatic steatosis OT - insulin resistance OT - leukotriene signaling OT - montelukast OT - oxidative stress COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:44 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - ph19101619 [pii] AID - pharmaceuticals-19-01619 [pii] AID - 10.3390/ph19101619 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 12;19(10):1619. doi: 10.3390/ph19101619. PMID- 42943311 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 11 TI - Capivasertib Combination Therapy in Advanced Breast Cancer and Other Solid Tumors: A Systematic Review and Meta-Analysis of Efficacy, Safety, and Biomarker-Defined Outcomes. LID - 10.3390/ph19101614 [doi] LID - 1614 AB - Background: Capivasertib is an oral pan-AKT inhibitor evaluated in combination with multiple systemic therapies across solid tumors, but its efficacy and safety vary by disease setting, biomarker status, and treatment backbone. We aimed to systematically evaluate the efficacy, safety, and early-phase clinical activity of capivasertib-containing combination regimens in advanced breast cancer and other solid tumors. Methods: MEDLINE, Web of Science, Scopus, Cochrane CENTRAL, trial registries, and major oncology conference sources were searched from inception to 31 May 2026. Randomized comparative studies, single-arm phase II studies, and phase I/Ib combination cohorts were included. Random-effects meta-analyses used restricted maximum likelihood estimation, with randomized and noncomparative evidence synthesized separately. Results: Twenty-seven reports representing 23 independent cohorts were included. In hormone receptor-positive/HER2-negative advanced breast cancer, capivasertib plus fulvestrant improved progression-free survival in the overall population (HR 0.59, 95% CI 0.51-0.69) and in PIK3CA/AKT1/PTEN-altered disease (HR 0.49, 95% CI 0.38-0.62), and increased objective response (RR 2.23, 95% CI 1.31-3.80). In triple-negative breast cancer, progression-free survival improved (HR 0.72, 95% CI 0.62-0.84), whereas overall survival remained inconclusive (HR 0.80, 95% CI 0.55-1.17). In PTEN-deficient metastatic hormone-sensitive prostate cancer, radiographic progression-free survival improved (HR 0.81, 95% CI 0.66-0.98). Capivasertib increased grade >/= 3 adverse events (RR 1.66, 95% CI 1.06-2.60) and treatment discontinuation (RR 2.97, 95% CI 1.27-6.96). Conclusions: Capivasertib demonstrated disease-, biomarker-, and treatment backbone-specific activity, with the most consistent comparative benefit observed in hormone receptor-positive/HER2-negative advanced breast cancer. Increased toxicity should be considered when selecting patients and treatment combinations. FAU - Alasmari, Moudi M AU - Alasmari MM AUID- ORCID: 0000-0002-2962-8737 AD - College of Medicine, King Saud Bin Abdulaziz University for Health Sciences (KSAU-HS), Jeddah 21556, Saudi Arabia. AD - King Abdullah International Medical Research Centre (KAIMRC), Jeddah 21556, Saudi Arabia. AD - Ministry of National Guard-Health Affairs (MNGHA), Jeddah 21556, Saudi Arabia. FAU - Aldossari, Rana M AU - Aldossari RM AD - Department of Pharmacology and Toxicology, College of Pharmacy, Prince Sattam Bin Abdelaziz University, Al Kharj 11942, Saudi Arabia. FAU - Aldriwesh, Marwh G AU - Aldriwesh MG AUID- ORCID: 0000-0001-6319-2669 AD - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud Bin Abdulaziz University for Health Sciences (KSAU-HS), Riyadh 11481, Saudi Arabia. AD - King Abdullah International Medical Research Centre (KAIMRC), Riyadh 11481, Saudi Arabia. AD - Ministry of National Guard-Health Affairs (MNGHA), Riyadh 11426, Saudi Arabia. FAU - Qadri, Marwa AU - Qadri M AUID- ORCID: 0000-0002-6548-411X AD - Department of Pharmacology and Toxicology, College of Pharmacy, Jazan University, Jazan 45142, Saudi Arabia. AD - Health Research Center (HRC), Jazan University, Jazan 45142, Saudi Arabia. FAU - Almaghrabi, Marey A AU - Almaghrabi MA AD - Department of Pharmaceutics and Pharmaceutical Industries, College of Pharmacy, Taibah University, Madinah 42353, Saudi Arabia. FAU - Alashari, Rawabi A AU - Alashari RA AUID- ORCID: 0009-0008-7991-6973 AD - Department of Clinical Pharmacology, Faculty of Medicine, King Abdulaziz University, Jeddah 21589, Saudi Arabia. LA - eng PT - Journal Article PT - Review DEP - 20261011 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708107 OTO - NOTNLM OT - AKT inhibition OT - PI3K/AKT/PTEN pathway OT - advanced breast cancer OT - capivasertib OT - meta-analysis OT - solid tumors COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/11 CRDT- 2026/10/27 10:44 PHST- 2026/09/04 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - ph19101614 [pii] AID - pharmaceuticals-19-01614 [pii] AID - 10.3390/ph19101614 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 11;19(10):1614. doi: 10.3390/ph19101614. PMID- 42943308 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 10 TI - Antiproliferative, Cytotoxic, and Antimigratory Effects of Licochalcone A in Ishikawa Endometrial Cancer Cells. LID - 10.3390/ph19101611 [doi] LID - 1611 AB - Background/Objectives: Licochalcone A (LA), a bioactive chalcone derived from Glycyrrhiza species, exhibits anticancer activity in several tumor models; however, its effects on endometrial cancer remain poorly understood. This study investigated the antiproliferative, cytotoxic, and antimigratory effects of LA in Ishikawa endometrial cancer cells and characterized associated changes in Akt/mTOR- and LC3-related molecular responses. Methods: Metabolic activity, colony formation, cell cycle distribution, migration, and invasion were evaluated following LA treatment. Ki-67 and LC3-II immunoreactivity, PI3K, Akt, mTOR, and MAP1LC3B transcript levels, and Akt phosphorylation were also assessed. Results: LA reduced metabolic activity and clonogenic capacity, decreased Ki-67 expression after prolonged exposure, increased apoptosis, and induced S-phase accumulation at higher concentrations. LA markedly reduced cell migration after 48 h, whereas no statistically significant change in invasion was detected. RT-qPCR revealed concentration- and time-dependent changes in PI3K, Akt1, mTOR, and MAP1LC3B transcript levels, with mTOR significantly reduced at all tested concentrations after 48 h. Akt phosphorylation was markedly reduced across all tested concentrations after 48 h, while MAP1LC3B transcript levels and LC3-II immunoreactivity showed concentration- and time-dependent changes. Conclusions: These findings demonstrate antiproliferative, cytotoxic, and antimigratory effects of LA in Ishikawa endometrial cancer cells and identify associated changes in Akt/mTOR- and LC3-related molecular responses. FAU - Ekiz-Yilmaz, Tugba AU - Ekiz-Yilmaz T AUID- ORCID: 0000-0002-9099-4779 AD - Department of Histology and Embryology, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34093, Turkey. FAU - Sag, Fatma Beyza AU - Sag FB AUID- ORCID: 0000-0003-4753-7748 AD - Department of Histology and Embryology, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34093, Turkey. LA - eng GR - TSA-2024-40521/Istanbul University/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708297 OTO - NOTNLM OT - Akt/mTOR OT - Ishikawa cells OT - Licochalcone A OT - cell death OT - cell proliferation OT - endometrial cancer OT - migration COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:44 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/12 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - ph19101611 [pii] AID - pharmaceuticals-19-01611 [pii] AID - 10.3390/ph19101611 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 10;19(10):1611. doi: 10.3390/ph19101611. PMID- 42943297 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 9 TI - Formulation Development of a pH-Adjusted, Preservative-Free 1% 5-Fluorouracil Eye Drop Compounded from Injectable Concentrate: A Risk-Assessed Magistral Formulation and 28-Day Stability Case Study. LID - 10.3390/ph19101600 [doi] LID - 1600 AB - Background: No licensed ophthalmic 5-fluorouracil (5-FU) product is available in Hungary, and published compounded 5-FU eye drops are alkaline dilutions of the injectable concentrate. We report a formulation development and 28-day storage-stability case study of a pH-adjusted, preservative-free 1% 5-FU eye drop prepared under Council of Europe Resolution CM/Res(2016)1. Methods: Five injectable concentrates were characterised for pH, and one was titrated with 0.1 N hydrochloric acid in a preservative-free ophthalmic vehicle to pH 7.30-7.60. Three 10 mL batches were prepared aseptically and release-tested; drop volume and delivered dose were determined on one batch, which was also followed for 28 days at 15-25 degrees C and 2-8 degrees C by inspection, pH, sterility and comparative HPLC-UV assay (single determinations). Results: All concentrates were alkaline (pH 8.95-9.18). The selected formulation reached pH 7.43; individual doses (mean 0.435 mg per drop) were within +/-8% of the mean. At 15-25 degrees C, samples remained clear and sterile, with pH 7.41-7.51 and assay >/= 97% of initial, without directional trend. At 2-8 degrees C, precipitate formed from day 7 and the supernatant assay fell to 89.1% by day 28. Conclusions: The preparation remained within specification for 28 days at controlled room temperature but not under refrigeration, consistent with, though not proven to result from, the lower solubility of un-ionised 5-FU. The 28-day period is provisional, not a validated shelf-life; in-use stability, osmolality and viscosity were not established. FAU - Vajda, Peter AU - Vajda P AUID- ORCID: 0000-0001-8788-482X AD - University Pharmacy Department of Pharmacy Administration, Faculty of Pharmacy, Semmelweis University, Hogyes Endre u. 7-9, 1092 Budapest, Hungary. FAU - Bartus, Gabor AU - Bartus G AD - University Pharmacy Department of Pharmacy Administration, Faculty of Pharmacy, Semmelweis University, Hogyes Endre u. 7-9, 1092 Budapest, Hungary. FAU - Csapo, Mate AU - Csapo M AD - University Pharmacy Department of Pharmacy Administration, Faculty of Pharmacy, Semmelweis University, Hogyes Endre u. 7-9, 1092 Budapest, Hungary. FAU - Horvath, Istvan Laszlo AU - Horvath IL AUID- ORCID: 0009-0001-4023-6099 AD - University Pharmacy Department of Pharmacy Administration, Faculty of Pharmacy, Semmelweis University, Hogyes Endre u. 7-9, 1092 Budapest, Hungary. FAU - Vajna, Marton AU - Vajna M AD - University Pharmacy Department of Pharmacy Administration, Faculty of Pharmacy, Semmelweis University, Hogyes Endre u. 7-9, 1092 Budapest, Hungary. FAU - Sebok, Szilvia AU - Sebok S AD - University Pharmacy Department of Pharmacy Administration, Faculty of Pharmacy, Semmelweis University, Hogyes Endre u. 7-9, 1092 Budapest, Hungary. FAU - Zelko, Romana AU - Zelko R AUID- ORCID: 0000-0002-5419-9137 AD - University Pharmacy Department of Pharmacy Administration, Faculty of Pharmacy, Semmelweis University, Hogyes Endre u. 7-9, 1092 Budapest, Hungary. LA - eng PT - Journal Article DEP - 20261009 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708713 OTO - NOTNLM OT - 5-fluorouracil OT - hospital pharmacy OT - magistral preparation OT - ocular surface squamous neoplasia OT - ophthalmic solution OT - pharmaceutical compounding OT - pharmacy practice OT - quality target product profile OT - risk assessment OT - stability OT - topical chemotherapy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:44 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - ph19101600 [pii] AID - pharmaceuticals-19-01600 [pii] AID - 10.3390/ph19101600 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 9;19(10):1600. doi: 10.3390/ph19101600. PMID- 42943293 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 9 TI - Polysaccharide Reengineering: Strategic Modifications and Biofunctional Outcomes of Natural Polysaccharides. LID - 10.3390/ph19101596 [doi] LID - 1596 AB - Natural polysaccharides have attracted considerable interest in pharmaceutical and biomedical applications. However, native polysaccharides often possess limitations, like poor solubility, inadequate mechanical strength, limited stability, and insufficient control over their functional and biological properties. Consequently, structural modification and function-driven reengineering of natural polysaccharides have emerged as important strategies for tailoring the physicochemical and biological characteristics to meet the requirements of advanced pharmaceutical and biomedical systems. Beyond individual modification strategies, polysaccharide reengineering provides a design-oriented framework in which structural features are deliberately tuned to establish relationships between molecular or network architecture, physicochemical properties, biointerfacial interactions, and biological function. This review examines polysaccharide reengineering as a function-driven approach to tailoring the structure and performance of natural polysaccharides. Rather than simply cataloguing modification methods, the review critically connects principal reengineering strategies, including carboxymethylation, sulfation, phosphorylation, grafting, and interpenetrating polymer network (IPN) formation, with their effects on structural characteristics, physicochemical properties, biointerfacial interactions, biological functions, and pharmaceutical performance. Particular emphasis is placed on identifying structure-property-biofunction relationships and on extracting design principles that can guide the selection and combination of reengineering strategies for specific applications. Reengineering of natural polysaccharides provides a versatile strategy to overcome the inherent limitations of native materials and to develop polysaccharide-based platforms with tailored physicochemical and biological properties. Different modification approaches offer distinct functional advantages: carboxymethylation can improve hydrophilicity and pH-responsive swelling, whereas sulfation and phosphorylation introduce anionic functionalities that modulate charge-dependent interactions and biological responses. Similarly, grafting and interpenetrating polymer network (IPN) formation provide greater control over mechanical strength, swelling, stability, drug-loading capacity, and controlled-release behavior. Collectively, these findings demonstrate that the performance of reengineered polysaccharides is determined not by the modification strategy alone, but by the combined effects of the resulting molecular structure, physicochemical characteristics, and interactions with biological environments. Despite these advances, the development of predictable structure-function relationships remains challenging because of variations in polysaccharide source, molecular weight, degree and distribution of modification, and characterization methodologies. Therefore, future research should shift from empirical modification toward rational, function-driven polysaccharide reengineering, in which structural parameters are deliberately controlled to achieve predefined material and biological outcomes. FAU - Lohani, Alka AU - Lohani A AUID- ORCID: 0000-0003-2580-7524 AD - Amity Institute of Pharmacy, Amity University, Noida 201313, Uttar Pradesh, India. FAU - Paliwal, Ajita AU - Paliwal A AD - Amity Institute of Pharmacy, Amity University, Noida 201313, Uttar Pradesh, India. FAU - Gautam, Arti AU - Gautam A AUID- ORCID: 0009-0004-4107-6666 AD - SBS Group of Educational and Training Institutes, College of Pharmacy, Fatehpur 212664, Uttar Pradesh, India. FAU - Figueiras, Ana AU - Figueiras A AUID- ORCID: 0000-0001-8170-1113 AD - Laboratory of Drug Development and Technologies, Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal. AD - REQUIMTE/LAQV, Group of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal. FAU - Mascarenhas-Melo, Filipa AU - Mascarenhas-Melo F AUID- ORCID: 0000-0003-0786-6280 AD - Higher School of Health, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal. AD - BRIDGES-Biotechnology Research, Innovation and Design for Health Products, Polytechnic University of Guarda, 6300-559 Guarda, Portugal. LA - eng PT - Journal Article PT - Review DEP - 20261009 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708429 OTO - NOTNLM OT - biological activity OT - chemical modification OT - grafting OT - pharmaceutical applications OT - polymer OT - polysaccharide modification COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:44 PHST- 2026/07/22 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - ph19101596 [pii] AID - pharmaceuticals-19-01596 [pii] AID - 10.3390/ph19101596 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 9;19(10):1596. doi: 10.3390/ph19101596. PMID- 42943290 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 8 TI - Inhibition of BAP1 Sensitizes Gastric Cancer Cells to 5-Fluorouracil by Promoting BIRC5 Degradation. LID - 10.3390/ph19101593 [doi] LID - 1593 AB - Background/Objectives: Gastric cancer is an aggressive malignancy with poor prognosis, and acquired resistance to 5-fluorouracil (5-FU) remains a major therapeutic challenge. BRCA1-associated protein-1 (BAP1), a deubiquitinating enzyme, has been implicated in tumorigenesis; however, its role in gastric cancer and 5-FU resistance remains unclear. This study investigated the functional and molecular roles of BAP1 in gastric cancer progression and chemoresistance. Methods: BAP1 expression and its association with patient prognosis were evaluated in gastric cancer tissues. The effects of BAP1 depletion on cancer cell proliferation, migration, invasion, and apoptosis were examined. The interaction between BAP1 and BIRC5 (Survivin) and the effects of BAP1-mediated deubiquitination on BIRC5 stability were investigated. The therapeutic effect of the BAP1 inhibitor iBAP-II was further assessed in 5-FU resistant gastric cancer cells. Results: BAP1 was significantly upregulated in gastric cancer tissues and associated with poor prognosis. BAP1 knockdown suppressed proliferation, migration, and invasion. Further analysis revealed that BAP1 interacted with and stabilized BIRC5 through deubiquitination, thereby suppressing apoptosis. BAP1 and BIRC5 were markedly upregulated in 5-FU resistant cells, whereas iBAP-II treatment enhanced 5-FU sensitivity and increased caspase-3 and PARP cleavage. Conclusions: BAP1 promotes gastric cancer progression and 5-FU resistance by stabilizing BIRC5. Targeting the BAP1-BIRC5 axis may represent a promising strategy for overcoming 5-FU resistance and improving therapeutic efficacy in gastric cancer. FAU - Jeong, In-Ho AU - Jeong IH AUID- ORCID: 0009-0001-0416-3937 AD - Department of Biochemistry and Molecular Biology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. FAU - Park, Yu-Jin AU - Park YJ AUID- ORCID: 0009-0000-9940-8986 AD - Department of Biochemistry and Molecular Biology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. FAU - Kim, Min-Jee AU - Kim MJ AUID- ORCID: 0000-0002-8081-6522 AD - Department of Biochemistry and Molecular Biology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. FAU - Lee, Eun Ju AU - Lee EJ AD - Department of Biochemistry and Molecular Biology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. FAU - Kim, Byung Sik AU - Kim BS AD - Department of General Surgery, Hallym University College of Medicine, Hallym Medical Center, Anyang 14068, Republic of Korea. FAU - Kim, Hee-Sung AU - Kim HS AD - Department of General Surgery, Hallym University College of Medicine, Hallym Medical Center, Anyang 14068, Republic of Korea. FAU - Lee, Peter Chang-Whan AU - Lee PC AUID- ORCID: 0000-0002-2320-6365 AD - Department of Biochemistry and Molecular Biology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. LA - eng GR - RS-2023-00208173/National Research Foundation of Korea/ PT - Journal Article DEP - 20261008 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708599 OTO - NOTNLM OT - 5-FU OT - BAP1 OT - BIRC5 OT - apoptosis OT - gastric cancer COIS- The authors declare that they have no competing interests. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:44 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - ph19101593 [pii] AID - pharmaceuticals-19-01593 [pii] AID - 10.3390/ph19101593 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 8;19(10):1593. doi: 10.3390/ph19101593. PMID- 42943284 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 8 TI - A Narrative Review of Cutaneous Wound-Healing Botanicals in South Africa: Integrating Ethnomedicinal Use, Pharmacological Evidence, and Conservation Prioritization. LID - 10.3390/ph19101587 [doi] LID - 1587 AB - African Traditional Medicine (ATM) remains an important component of primary healthcare in South Africa; however, evidence for plants used in cutaneous wound care is dispersed across ethnobotanical, pharmacological, toxicological, and conservation literature. This narrative review integrates these evidence streams and proposes a conservation- and safety-aware translational prioritization framework for wound-healing botanicals used in South African ethnomedical practice. A structured search of PubMed, ScienceDirect, Web of Science, Google Scholar, and SciFinder identified eligible peer-reviewed ethnobotanical and preclinical studies published between January 1999 and June 2026. After taxonomic harmonization, 173 accepted taxa were documented from South African ethnobotanical reports as being traditionally used for wound care. Only seven taxa had direct wound closure evidence in relevant in vitro or in vivo models, while twenty-eight taxa had wound-relevant anti-inflammatory evidence. Reported mechanisms included keratinocyte and fibroblast migration, collagen-associated responses, angiogenesis, wound contraction, and inflammatory modulation. The evidence base remains uneven: many taxa are supported primarily by traditional use, while dose, formulation, treatment frequency, duration of use, dermal safety, and sustainable sourcing are poorly reported. Safety concerns were identified for selected toxic or irritant taxa, and destructive harvesting of roots, bulbs, corms, rhizomes, and bark may increase conservation risk. Overall, the review highlights a substantial gap between ethnomedical documentation and pharmacological validation, and it provides a prioritization strategy to guide future phytochemical, mechanistic, formulation, dermal-safety, and preclinical wound-healing research in South Africa. FAU - Sithole, Siboniso AU - Sithole S AUID- ORCID: 0000-0002-6290-6559 AD - Sub-Discipline of Traditional Medicine, School of Medicine, College of Health Sciences, University of KwaZulu-Natal, Durban 4041, South Africa. FAU - Mateta, Carl AU - Mateta C AUID- ORCID: 0009-0001-8362-3582 AD - National Institute of Health Research, Ministry of Health and Child Care, Harare P.O. Box CY 1122, Zimbabwe. FAU - Hlatshwayo, Sphamandla AU - Hlatshwayo S AUID- ORCID: 0000-0002-2655-5341 AD - Sub-Discipline of Traditional Medicine, School of Medicine, College of Health Sciences, University of KwaZulu-Natal, Durban 4041, South Africa. FAU - Mhlungu, Sanele AU - Mhlungu S AD - Sub-Discipline of Traditional Medicine, School of Medicine, College of Health Sciences, University of KwaZulu-Natal, Durban 4041, South Africa. FAU - Thembane, Nokukhanya AU - Thembane N AUID- ORCID: 0000-0001-9146-3809 AD - Sub-Discipline of Traditional Medicine, School of Medicine, College of Health Sciences, University of KwaZulu-Natal, Durban 4041, South Africa. AD - Department of Biomedical Sciences, Faculty of Applied and Health Sciences, Mangosuthu University of Technology, Durban 4026, South Africa. FAU - Afrika, Phumzile AU - Afrika P AD - Sub-Discipline of Traditional Medicine, School of Medicine, College of Health Sciences, University of KwaZulu-Natal, Durban 4041, South Africa. FAU - Senzani, Sibusiso AU - Senzani S AD - Discipline of Medical Microbiology, School of Medicine, College of Health Sciences, University of KwaZulu-Natal, Durban 4000, South Africa. FAU - Gomo, Exnevia AU - Gomo E AUID- ORCID: 0000-0003-0891-6251 AD - Medical Laboratory Sciences Unit, Faculty of Medicine and Health Sciences, University of Zimbabwe, Mount Pleasant, Harare P.O. Box MP 167, Zimbabwe. FAU - Gqaleni, Nceba AU - Gqaleni N AD - Sub-Discipline of Traditional Medicine, School of Medicine, College of Health Sciences, University of KwaZulu-Natal, Durban 4041, South Africa. AD - Africa Health Research Institute, Nelson R. Mandela School of Medicine, 3rd Floor K-RITH Tower Building, Durban 4001, South Africa. FAU - Ngcobo, Mlungisi AU - Ngcobo M AUID- ORCID: 0000-0001-8942-1642 AD - Sub-Discipline of Traditional Medicine, School of Medicine, College of Health Sciences, University of KwaZulu-Natal, Durban 4041, South Africa. LA - eng GR - MND210618613140/National Research Foundation/ PT - Journal Article PT - Review DEP - 20261008 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708376 OTO - NOTNLM OT - South Africa OT - conservation status OT - ethnobotany OT - ethnopharmacology OT - medicinal plants OT - traditional medicine OT - wound healing COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:44 PHST- 2026/07/28 00:00 [received] PHST- 2026/09/04 00:00 [revised] PHST- 2026/09/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - ph19101587 [pii] AID - pharmaceuticals-19-01587 [pii] AID - 10.3390/ph19101587 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 8;19(10):1587. doi: 10.3390/ph19101587. PMID- 42943279 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 6 TI - N-Adamantyl-1-Pentyl-4-Oxo-1,4-Dihydroquinoline-3-Carboxamide and N-(Adamantan-1-Yl)-2-(Alkyloxy)benzamide Derivatives as Fluorescent Probes for Selective CB2R Targeting. LID - 10.3390/ph19101582 [doi] LID - 1582 AB - Background/Objectives: The cannabinoid receptor subtype 2 (CB2R) is an increasingly important therapeutic and diagnostic target because of its involvement in inflammation-associated disorders, including cancer and neurodegenerative diseases. CB2R biology has traditionally been investigated using radioligands, whose broader application is limited by safety, regulatory, operational, and environmental constraints. Fluorescent ligands provide a complementary approach for real-time, high-resolution analysis of receptor expression and dynamics in living systems. This study aimed to develop structurally diverse fluorescent CB2R ligands as molecular tools for investigating receptor localization, trafficking, and signaling across imaging and cell-based platforms. Methods: Probe design was based on two complementary CB2R chemotypes: the 4-oxoquinolinone scaffold of the previously reported fluorescent ligands SM15 and VM7 and the salicylamide scaffold of the reference ligand CC48. These pharmacophores were conjugated to Cyanine 5 (Cy5), Cyanine 7 (Cy7), BODIPY-TR, 7-nitrobenzo-2-oxa-1,3-diazole (NBD), or 7-nitrobenzo-2-selena-1,3-diazole (Se-NBD), generating ligands with emission profiles spanning the green to near-infrared spectral regions. Compound 2, which showed the highest CB2R affinity among the new ligands (K(i) = 143.4 nM) and negligible CB1R displacement at 1 microM, was characterized photophysically in organic and aqueous media and evaluated by flow cytometry in MCF7 and PANC-1 cancer cells, which display low and high CB2R expression, respectively. Results: Compound 2 retained nanomolar CB2R affinity and exhibited detectable fluorescence in organic solvents and aqueous buffer down to nanomolar concentrations. In flow cytometry experiments, its concentration-dependent fluorescence differentiated MCF7 from PANC-1 cells. Partial displacement by the CB2R reference ligand 13, particularly in PANC-1 cells, supported a receptor-specific component of the observed labeling. Conclusions: Compound 2 combines CB2R selectivity with robust fluorescence across chemically distinct environments and can distinguish cellular models with different receptor-expression levels. These findings establish compound 2 as a promising tool for CB2R detection and provide a rational basis for developing optimized fluorescent ligands for receptor imaging, target validation, and related translational applications. FAU - Musillo, Gabriella Rosanna AU - Musillo GR AD - Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Via E. Orabona, 4, 70125 Bari, Italy. FAU - Graziano, Giovanni AU - Graziano G AUID- ORCID: 0000-0002-5575-3240 AD - Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Via E. Orabona, 4, 70125 Bari, Italy. FAU - Mammone, Mariachiara AU - Mammone M AD - Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Via E. Orabona, 4, 70125 Bari, Italy. FAU - Mastropasqua, Francesco AU - Mastropasqua F AUID- ORCID: 0009-0004-7136-2807 AD - Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Via E. Orabona, 4, 70125 Bari, Italy. FAU - Majellaro, Maria AU - Majellaro M AUID- ORCID: 0000-0003-3490-7703 AD - Centro Singular de Investigacion en Quimica Bioloxica y Materiais Moleculares (CiQUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain. AD - Departamento de Quimica Organica, Facultade de Farmacia, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain. FAU - Gioe-Gallo, Claudia AU - Gioe-Gallo C AD - Centro Singular de Investigacion en Quimica Bioloxica y Materiais Moleculares (CiQUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain. AD - Departamento de Quimica Organica, Facultade de Farmacia, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain. FAU - Brea, Jose AU - Brea J AUID- ORCID: 0000-0002-5523-1979 AD - Innopharma Screening Platform, BioFarma Research Group, Center for Research in Molecular Medicine and Chronic Diseases (CiMUS), University of Santiago de Compostela, 15782 Santiago de Compostela, Spain. AD - Department of Pharmacology, Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain. FAU - Loza, Maria Isabel AU - Loza MI AD - Innopharma Screening Platform, BioFarma Research Group, Center for Research in Molecular Medicine and Chronic Diseases (CiMUS), University of Santiago de Compostela, 15782 Santiago de Compostela, Spain. AD - Department of Pharmacology, Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain. FAU - Liturri, Anselma AU - Liturri A AUID- ORCID: 0009-0005-3291-8910 AD - Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Via E. Orabona, 4, 70125 Bari, Italy. FAU - Miciaccia, Morena AU - Miciaccia M AUID- ORCID: 0000-0002-0727-6435 AD - Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Via E. Orabona, 4, 70125 Bari, Italy. FAU - Perrone, Maria Grazia AU - Perrone MG AUID- ORCID: 0000-0003-4195-5228 AD - Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Via E. Orabona, 4, 70125 Bari, Italy. FAU - Stefanachi, Angela AU - Stefanachi A AUID- ORCID: 0000-0002-9430-7972 AD - Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Via E. Orabona, 4, 70125 Bari, Italy. FAU - Colella, Matilde AU - Colella M AUID- ORCID: 0000-0002-9584-7030 AD - Department of Biosciences, Biotechnologies and Environment, University of Bari Aldo Moro, Via E. Orabona, 4, 70125 Bari, Italy. FAU - Alberga, Domenico AU - Alberga D AUID- ORCID: 0000-0002-9475-9602 AD - Institute of Crystallography, National Research Council of Italy, 70126 Bari, Italy. FAU - Sotelo, Eddy AU - Sotelo E AUID- ORCID: 0000-0001-5571-2812 AD - Centro Singular de Investigacion en Quimica Bioloxica y Materiais Moleculares (CiQUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain. AD - Departamento de Quimica Organica, Facultade de Farmacia, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain. FAU - Colabufo, Nicola Antonio AU - Colabufo NA AUID- ORCID: 0000-0001-5639-7746 AD - Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Via E. Orabona, 4, 70125 Bari, Italy. FAU - Contino, Marialessandra AU - Contino M AUID- ORCID: 0000-0002-0713-3151 AD - Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Via E. Orabona, 4, 70125 Bari, Italy. FAU - Abate, Carmen AU - Abate C AUID- ORCID: 0000-0001-9292-884X AD - Department of Pharmacy-Pharmaceutical Sciences, University of Bari Aldo Moro, Via E. Orabona, 4, 70125 Bari, Italy. LA - eng PT - Journal Article DEP - 20261006 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708458 OTO - NOTNLM OT - cannabinoid receptor subtype 2 OT - diagnostics OT - flow cytometry OT - fluorescent probes OT - quinolinone derivatives COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/06 CRDT- 2026/10/27 10:44 PHST- 2026/07/31 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/06 00:00 [pmc-release] AID - ph19101582 [pii] AID - pharmaceuticals-19-01582 [pii] AID - 10.3390/ph19101582 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 6;19(10):1582. doi: 10.3390/ph19101582. PMID- 42943275 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 5 TI - Drugging the Undruggable in Oncology: Structural Computation, Machine Learning, and Generative AI for Historically Intractable Targets. LID - 10.3390/ph19101578 [doi] LID - 1578 AB - For four decades the central oncogenic drivers of human cancer the RAS GTPases, the transcription-factor MYC, mutant p53, and the protein-tyrosine phosphatases, fusion oncoproteins and intrinsically disordered regulators that surround them were regarded as undruggable: lacking deep, well-defined small-molecule binding pockets, governed by femtomolar substrate affinities, or driven through flat protein-protein interfaces that classical medicinal chemistry could not engage. The last decade has overturned that verdict. This narrative review argues that the convergence of three advances has made the undruggable proteome systematically approachable: the discovery of cryptic, allosteric and induced pockets through molecular dynamics and deep learning; the maturation of physics-based and machine-learned models that can read out binding from structure; and the rise in generative artificial intelligence that can design novel molecules conditioned directly on a three-dimensional target. We develop the argument in full formal detail. We set out the mathematics of modern molecular machine learning E(3)/SE(3)-equivariant graph neural networks, denoising diffusion and score-based generative models, and generative flow networks alongside the statistical mechanics of binding free energy (free-energy perturbation, thermodynamic integration, MM-PBSA), the kinetics of covalent inhibition, the equilibrium theory of ternary complexes and cooperativity that governs molecular glues and degraders, and the pharmacology that determines whether a designed molecule reaches and acts on a tumour. We then examine, target by target, the biology that made each oncoprotein intractable and the campaigns structural, chemical and increasingly computational that have begun to defeat it, distinguishing throughout what classical structural and computational work achieved, what machine learning demonstrably contributed, and what generative design has so far only promised: the switch-II pocket and the covalent KRAS-G12C and non-covalent KRAS-G12D and pan-RAS(ON) inhibitors; the mutation-created cavity of p53-Y220C and its first reactivators; the disordered MYC-MAX interface and the miniprotein Omomyc; and the allosteric tunnel of SHP2. These four are selected for mechanistic non-redundancy, on grounds we state explicitly, and the structured search framework and eligibility criteria underlying the review are documented. We foreground the newest evidence AlphaFold3, RoseTTAFold All-Atom, deep cryptic-pocket predictors, and the first generative-to-clinic existence proofs while maintaining candour about the field's hard limits: the physical-validity and generalisation gaps of deep docking, the bounded accuracy and cost of free-energy methods, the synthesizability gap, dual-use risk, and a clinical attrition that artificial intelligence has not yet reduced. The synthesis we offer is not that the problem is solved but that it has changed in kind: from a search for the rare tractable target to the deliberate, computable engineering of molecules against targets once thought beyond reach. FAU - Stanczak, Mikolaj AU - Stanczak M AUID- ORCID: 0009-0007-3931-0566 AD - School of Mathematical and Computer Science, Heriot-Watt University, Edinburgh EH14 4AS, UK. FAU - Niazi, Sarfaraz AU - Niazi S AUID- ORCID: 0000-0002-0513-0336 AD - College of Pharmacy and Pharmaceutical Sciences, Washington State University, Spokane, WA 99202, USA. LA - eng PT - Journal Article PT - Review DEP - 20261005 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708652 OTO - NOTNLM OT - AlphaFold OT - KRAS OT - MYC OT - SHP2 OT - covalent inhibition OT - cryptic pockets OT - diffusion models OT - equivariant neural networks OT - free-energy perturbation OT - generative chemistry OT - molecular glues OT - p53 OT - pan-cancer drug discovery OT - targeted protein degradation OT - undruggable targets COIS- S.N. is an equity owner in several companies developing biological drugs, but none of these is competing with the subject of this manuscript. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/05 CRDT- 2026/10/27 10:44 PHST- 2026/09/01 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/05 00:00 [pmc-release] AID - ph19101578 [pii] AID - pharmaceuticals-19-01578 [pii] AID - 10.3390/ph19101578 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 5;19(10):1578. doi: 10.3390/ph19101578. PMID- 42943273 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 4 TI - Indirect Skin Homeostasis Modulation by an Essential Oil Blend in Mice with Insomnia Comorbid Anxiety: Sensitivity of Dermal Fibroblasts to Exogenously Applied Circulating Molecules. LID - 10.3390/ph19101576 [doi] LID - 1576 AB - Background/Objectives: Studies have confirmed that essential oils extracted from aromatic plants exert mood-regulating and skin-beneficial effects. Although the concept of "emotional skincare" has attracted growing research interest, experimental evidence supporting the indirect skin-protective effects of essential oils through emotional regulation remains limited. This study investigates the indirect effect of an essential oil blend (EOB) on skin homeostasis in mice with insomnia comorbid anxiety (ICA, a pharmacologically validated emotional stress model induced by para-chlorophenylalanine) and aims to profile the sensitivity of dermal fibroblasts to candidate circulating molecules identified from EOB-treated ICA mice, thereby laying a mechanistic foundation for emotional skincare research. Methods: An essential oil blend (EOB) comprising lavender, Kushui rose, sweet orange, Roman chamomile, sandalwood, and clary sage essential oils was prepared. An ICA mouse model was established using para-chlorophenylalanine. Behavioral phenotypes, as well as neurotransmitter and hormonal indices, were measured to validate the anxiolytic and sleep disturbance-alleviating effects of EOB administered via intraperitoneal injection at 300 mg/kg per injection (two injections overall). Skin histological alterations and circulating biomarker profiles were evaluated, and selected differentially altered circulating molecules were exogenously applied to cultured dermal fibroblasts in vitro to assess their effects on fibroblast functions. Results: EOB significantly alleviated ICA-like behaviors and ameliorated abnormal levels of brain neurotransmitters and hormones in mice. Although only mild histological variations were observed in the skin after EOB intervention, significant alterations in cortisol, melatonin, 5-hydroxytryptamine, interleukin-10, and type I collagen were detected in serum or skin tissues. Exogenous application of these circulating molecules elicited differential responses in dermal fibroblasts, affecting oxidative stress, inflammatory responses, and extracellular matrix synthesis in a molecule-specific manner. Notably, these fibroblast assays only demonstrate cellular sensitivity in vitro and do not prove that these circulating molecules directly mediate the in vivo effects observed in mice. Conclusions: EOB intervention ameliorates insomnia- and anxiety-related behaviors and concurrent favorable changes in skin-associated molecular profiles in ICA mice. These changes are accompanied by alterations in circulating bioactive molecules to which dermal fibroblasts exhibit measurable sensitivity in vitro. These observations are consistent with, but do not definitively establish, a brain-skin axis-mediated mechanism. These findings provide preliminary experimental evidence for the emotional skincare concept and identify candidate circulating molecules for future causal validation; the in vivo regulatory roles of these molecules and the precise signaling pathways involved remain to be established in dedicated mechanistic studies. FAU - Liu, Fei AU - Liu F AUID- ORCID: 0000-0002-8505-6227 AD - Shandong Freda Biotech Co., Ltd., No. 888 Xinluo Street, High-Tech Zone, Jinan 250101, China. FAU - Xu, Jiamin AU - Xu J AD - Department of Landscape Architecture, School of Design, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China. FAU - Chen, Zimeng AU - Chen Z AUID- ORCID: 0000-0001-7245-2004 AD - Department of Landscape Architecture, School of Design, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China. FAU - Yao, Lei AU - Yao L AD - Department of Landscape Architecture, School of Design, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China. FAU - Li, Yuhong AU - Li Y AD - Department of Landscape Architecture, School of Design, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China. FAU - Wang, Tiange AU - Wang T AD - Shandong Freda Biotech Co., Ltd., No. 888 Xinluo Street, High-Tech Zone, Jinan 250101, China. FAU - Wang, Qian AU - Wang Q AD - Shandong Freda Biotech Co., Ltd., No. 888 Xinluo Street, High-Tech Zone, Jinan 250101, China. FAU - He, Jing AU - He J AD - Xinjiang Eprhan Spice Co., Ltd., No. 567 South Tianshan Road, Kekedala 835219, China. FAU - Yang, Suzhen AU - Yang S AD - Shandong Freda Biotech Co., Ltd., No. 888 Xinluo Street, High-Tech Zone, Jinan 250101, China. FAU - Wang, Wencui AU - Wang W AD - Department of Landscape Architecture, School of Design, Shanghai Jiao Tong University, No. 800 Dongchuan Road, Shanghai 200240, China. LA - eng GR - 2025AB044/Xinjiang Production and Construction Corps/ PT - Journal Article DEP - 20261004 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708607 OTO - NOTNLM OT - emotional skincare OT - essential oil blend OT - extracellular matrix OT - insomnia comorbid anxiety OT - oxidative stress COIS- Authors Fei Liu, Tiange Wang, Qian Wang and Suzhen Yang were employed by the company Shandong Freda Biotech Co., Ltd. Author Jing He was employed by the company Xinjiang Eprhan Spice Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/04 CRDT- 2026/10/27 10:44 PHST- 2026/07/18 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/04 00:00 [pmc-release] AID - ph19101576 [pii] AID - pharmaceuticals-19-01576 [pii] AID - 10.3390/ph19101576 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 4;19(10):1576. doi: 10.3390/ph19101576. PMID- 42943271 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 4 TI - Effects of Endocannabinoid System Modulation on Cholinergic-Induced Anxiety-like Behaviors in Mice. LID - 10.3390/ph19101574 [doi] LID - 1574 AB - Background: Anxiety disorders are clinically significant non-cognitive symptoms of many neuropsychiatric disorders, often accompanying progressive memory deficits and reflecting neurochemical dysregulation, particularly cholinergic dysfunction. Current therapy for these disorders is focused on the memory deficits but does not improve emotional symptoms and may even worsen anxiety. Therefore, novel therapeutic strategies are needed that address both cognitive and non-cognitive symptoms. Aim: Recent studies suggest that the endocannabinoid system (ECS) may play a key role in regulating cognitive and emotional processes, including anxiety. The aim of the study was to evaluate how direct (via selective ligands of cannabinoid CB1 and CB2 receptors) and indirect (via inhibitors of endocannabinoid-degrading enzymes-fatty acid amide hydrolase (FAAH) and monoacylglycerol lipase (MAGL)) modulation of ECS activity affects anxiety-like behaviors induced by pharmacological modification of cholinergic neurotransmission (by using nicotine or scopolamine). Methods: Anxiety-related behaviors were assessed in mice using the elevated plus maze (EPM) test. Results: CB1 receptor agonist oleamide (5 mg/kg), FAAH inhibitor URB 597 (0.1 mg/kg), and MAGL inhibitor JZL 184 (20 mg/kg) attenuated the anxiogenic effects of nicotine (0.1 mg/kg). Additionally, oleamide (5 mg/kg), CB1/CB2 receptor agonist WIN 55,212-2 (0.25 mg/kg), CB2 receptor antagonist AM 630 (0.25 mg/kg), and JZL 184 (20 mg/kg) attenuated the anxiogenic effects induced by scopolamine (1 mg/kg). Conclusions: Both direct and indirect ECS modulation demonstrated an impact on anxiety-like responses induced by cholinergic ligands. This potential relationship between ECS and cholinergic pathways provides additional insights into anxiety regulation and may represent a promising starting point for further research into targeted anxiolytic mechanisms in the central nervous system (CNS). FAU - Kruk-Slomka, Marta AU - Kruk-Slomka M AUID- ORCID: 0000-0001-9201-7181 AD - Department of Pharmacology and Pharmacodynamics, Medical University of Lublin, 4a Chodzki Str., 20-093 Lublin, Poland. AD - Experimental Medicine Center (OMD), Medical University of Lublin, Jaczewskiego 8D, 20-090 Lublin, Poland. FAU - Slomka, Tomasz AU - Slomka T AD - Department of Medical Informatics and Statistics with E-Health Lab, Medical University of Lublin, Jaczewskiego 4 Street, 20-954 Lublin, Poland. FAU - Dzik, Agnieszka AU - Dzik A AD - Department of Pharmacology and Pharmacodynamics, Medical University of Lublin, 4a Chodzki Str., 20-093 Lublin, Poland. AD - Experimental Medicine Center (OMD), Medical University of Lublin, Jaczewskiego 8D, 20-090 Lublin, Poland. FAU - Biala, Grazyna AU - Biala G AD - Department of Pharmacology and Pharmacodynamics, Medical University of Lublin, 4a Chodzki Str., 20-093 Lublin, Poland. AD - Experimental Medicine Center (OMD), Medical University of Lublin, Jaczewskiego 8D, 20-090 Lublin, Poland. LA - eng GR - DS 21/Statutory Funds of Medical University of Lublin/ GR - DS 23/Statutory Funds of Medical University of Lublin/ PT - Journal Article DEP - 20261004 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708551 OTO - NOTNLM OT - anxiety OT - cholinergic pathways OT - elevated plus maze test OT - endocannabinoid system modulation OT - mice COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/04 CRDT- 2026/10/27 10:44 PHST- 2026/07/06 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/04 00:00 [pmc-release] AID - ph19101574 [pii] AID - pharmaceuticals-19-01574 [pii] AID - 10.3390/ph19101574 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 4;19(10):1574. doi: 10.3390/ph19101574. PMID- 42943269 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 3 TI - Combination Therapy Potential of HTD1801 (Berberine Ursodeoxycholate) in MASLD/MASH and Cardiovascular-Kidney-Metabolic Disease: A Narrative Review. LID - 10.3390/ph19101572 [doi] LID - 1572 AB - Background/Objectives: The cardiovascular-kidney-metabolic (CKM) continuum is characterized by the close interrelatedness of metabolic, hepatic, cardiovascular, and renal disease, making multidomain therapies particularly relevant to MASLD/MASH. HTD1801, an investigational ionic therapeutic, combines berberine's metabolic effects with the hepatoprotective effects of ursodeoxycholic acid (UDCA), making it a potential multidomain therapy within this setting. This review had two related goals: first, to synthesize the mechanistic and clinical evidence for HTD1801 itself; and second, to evaluate the rationale and available evidence for extending this complementary-action approach through combination with other therapies relevant to MASLD/MASH and CKM disease. Methods: PubMed/MEDLINE, ClinicalTrials.gov, conference abstracts, and selected regulatory or sponsor sources were reviewed through 9 August 2026. Six CKM-relevant therapeutic classes were selected for their human evidence of multidomain benefit: metformin, GLP-1 receptor agonists, statins, SGLT2 inhibitors, PPAR agonists, and resmetirom. Studies of both HTD1801 and its individual components, berberine and UDCA, were included. A systematic review or meta-analysis was not feasible due to the limited and heterogeneous evidence base, instead necessitatinganarrative review. Results: Across seven completed HTD1801 clinical studies with publicly reported results, treatment showed multidomain metabolic and hepatic benefits across the CKM spectrum, including improvements in HbA1c, LDL-C, MRI-PDFF hepatic fat, ALT, AST, and GGT. Direct human evidence: HTD1801 + metformin, supported by a Phase 3 randomized add-on trial. Indirect human evidence: HTD1801 + SGLT2 inhibitors. Preclinical evidence: HTD1801 + GLP-1 receptor agonists; berberine + statins. Theoretical mechanistic rationale only: HTD1801 + PPAR agonists; HTD1801 + resmetirom. Overall, mechanistic and preclinical rationale has advanced further than direct human validation, and evidence remains centered largely on surrogate rather than definitive clinical outcomes. Conclusions: HTD1801 has demonstrated multidomain metabolic and hepatic activity, but direct human evidence supporting its use with other CKM therapies remains limited and is currently established only for metformin. The principal next step is to test HTD1801 in dedicated combination RCTs for clinically meaningful benefit. Further studies should characterize combination-specific safety and tolerability and compare HTD1801 with separate berberine and UDCA administration. FAU - Kim, Boknyun AU - Kim B AD - Virginia Headquarters Campus, Virginia University of Integrative Medicine, 1980 Gallows Road, Vienna, VA 22182, USA. AD - Greater Los Angeles Science Foundation, Los Angeles, CA 90405, USA. FAU - Kim, Joseph AU - Kim J AUID- ORCID: 0009-0003-3807-3387 AD - Greater Los Angeles Science Foundation, Los Angeles, CA 90405, USA. AD - Human Stem Cell and Genome Engineering Center, University of California, Los Angeles, CHS 36-125/133/143 CHS38-133, 650 Charles E Young Dr S, Los Angeles, CA 90095, USA. AD - Thomas C. Jenkins Department of Biophysics, Johns Hopkins University, 110 Jenkins Hall, 3400 N. Charles Street, Baltimore, MD 21218, USA. AD - Department of Computer Science, Johns Hopkins University, 160 Malone Hall, 3400 N. Charles Street, Baltimore, MD 21218, USA. FAU - Kim, Grace AU - Kim G AUID- ORCID: 0009-0001-0881-1276 AD - Greater Los Angeles Science Foundation, Los Angeles, CA 90405, USA. AD - Life Sciences Department, Santa Monica College, 1900 Pico Boulevard, Santa Monica, CA 90405, USA. FAU - Kim, Kitai AU - Kim K AD - Virginia Headquarters Campus, Virginia University of Integrative Medicine, 1980 Gallows Road, Vienna, VA 22182, USA. AD - Greater Los Angeles Science Foundation, Los Angeles, CA 90405, USA. AD - Human Stem Cell and Genome Engineering Center, University of California, Los Angeles, CHS 36-125/133/143 CHS38-133, 650 Charles E Young Dr S, Los Angeles, CA 90095, USA. LA - eng PT - Journal Article PT - Review DEP - 20261003 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708656 OTO - NOTNLM OT - HTD1801 OT - berberine ursodeoxycholate OT - cardiovascular-kidney-metabolic syndrome OT - combination therapy OT - metabolic dysfunction-associated steatohepatitis OT - metabolic dysfunction-associated steatotic liver disease OT - type 2 diabetes mellitus COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/03 CRDT- 2026/10/27 10:44 PHST- 2026/09/07 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - ph19101572 [pii] AID - pharmaceuticals-19-01572 [pii] AID - 10.3390/ph19101572 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 3;19(10):1572. doi: 10.3390/ph19101572. PMID- 42943268 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 3 TI - Exploring Chemical Diversity Alongside Antioxidant, Antimicrobial, Antiproliferative, Anti-Inflammatory, and Caenorhabditis elegans Locomotor Responses in Whole, Caps and Stipes of Suillus luteus and Agaricus bisporus. LID - 10.3390/ph19101571 [doi] LID - 1571 AB - Edible mushrooms contain chemically diverse metabolites that can be characterised for their biological response under experimental conditions. This study characterised whole mushrooms, including caps and stipes, from wild Suillus luteus and cultured Agaricus bisporus var. brunnescens, and evaluated their physicochemical composition, bioactive compounds, and antioxidant, antimicrobial, antiproliferative, anti-inflammatory, and locomotor activities in Caenorhabditis elegans. The extracts were evaluated using spectrophotometric, chromatographic, ABTS, and DPPH assays, as well as microdilution, MTT, nitrite production in RAW 264.7 macrophages, and locomotor parameters. Agaricus bisporus var. brunnescens contained 46.7 and 50.2 mg/100 g of vitamin C in the whole mushroom and the cap, respectively, and between 1685.9 and 2152.8 mg/100 g DW of total phenols. S. luteus contained 38,609.0-51,974.6 mg/100 g DW of total organic acids, primarily malic acid, and exhibited higher antioxidant capacity ranging from 3.2 to 3.3 mmol TE/100 g DW as measured by the DPPH assay and 4.9-5.9 as measured by the ABTS assay. A. bisporus showed growth-inhibitory activity against all eight bacterial strains tested and exhibited the lowest MIC against Staphylococcus aureus (3.9 mg/mL), whereas no inhibition was detected against the two Candida strains tested. S. luteus showed an IC(50) of 0.08-0.21 mg/mL, although with limited tumour selectivity, and reduced LPS-induced nitrite levels by up to 64.6%. The locomotor changes observed in C. elegans were not statistically significant. These results are exploratory and serve as a starting point for future research. FAU - Coyago-Cruz, Elena AU - Coyago-Cruz E AUID- ORCID: 0000-0003-4495-3500 AD - Carrera de Ingenieria en Biotecnologia, Universidad Politecnica Salesiana, Sede Quito, Campus El Giron, Av. 12 de Octubre N2422 y Wilson, Quito 170143, Ecuador. FAU - Puente-Pineda, Juan A AU - Puente-Pineda JA AUID- ORCID: 0009-0005-3881-9200 AD - Centro de Investigacion Biomedica (CENBIO), Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito 170527, Ecuador. FAU - Gonzalez-Pastor, Rebeca AU - Gonzalez-Pastor R AUID- ORCID: 0000-0002-5630-6645 AD - Centro de Investigacion Biomedica (CENBIO), Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito 170527, Ecuador. FAU - Soledispa, Julieth AU - Soledispa J AUID- ORCID: 0009-0005-1797-3268 AD - Carrera de Ingenieria en Biotecnologia, Universidad Politecnica Salesiana, Sede Quito, Campus El Giron, Av. 12 de Octubre N2422 y Wilson, Quito 170143, Ecuador. FAU - Cobos, Angelica Geovanna Zea AU - Cobos AGZ AUID- ORCID: 0000-0002-6293-0735 AD - Grupo de Innovacion Educativa, Seguridad y Soberania Alimentaria (GIE-SYSA), Universidad Politecnica Salesiana, Sede Cuenca, Av. Turuhuayco 3-69 y Calle Vieja, Cuenca 010150, Ecuador. FAU - Carrillo-Naranjo, Oscar AU - Carrillo-Naranjo O AD - Centro de Investigacion Biomedica (CENBIO), Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito 170527, Ecuador. FAU - Barba-Ostria, Carlos AU - Barba-Ostria C AUID- ORCID: 0000-0001-8129-233X AD - Escuela de Medicina, Colegio de Ciencias de la Salud Quito, Universidad San Francisco de Quito (USFQ), Quito 170901, Ecuador. AD - Instituto de Microbiologia, Universidad San Francisco de Quito (USFQ), Quito 170901, Ecuador. FAU - Heredia-Moya, Jorge AU - Heredia-Moya J AUID- ORCID: 0000-0001-7620-9140 AD - Centro de Investigacion Biomedica (CENBIO), Facultad de Ciencias de la Salud Eugenio Espejo, Universidad UTE, Quito 170527, Ecuador. FAU - Tafur, Gina AU - Tafur G AD - Carrera de Ingenieria Agropecuaria, Universidad Politecnica Salesiana, Sede Quito, Campus Cayambe, Av. Natalia Jarrin N3-85 y 9 de Octubre, Cayambe 170501, Ecuador. LA - eng PT - Journal Article DEP - 20261003 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708346 OTO - NOTNLM OT - antiproliferative effects OT - edible mushrooms OT - phenolics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/03 CRDT- 2026/10/27 10:44 PHST- 2026/09/05 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - ph19101571 [pii] AID - pharmaceuticals-19-01571 [pii] AID - 10.3390/ph19101571 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 3;19(10):1571. doi: 10.3390/ph19101571. PMID- 42943266 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 2 TI - In Silico Evaluation of Cyanobacteria-Derived Bioactive Compounds as Potential Monoamine Oxidase-B Inhibitors for Parkinson's Disease: Molecular Docking and Molecular Dynamics Simulation Study. LID - 10.3390/ph19101569 [doi] LID - 1569 AB - Background/Objectives: Parkinson's disease (PD) is a progressive neurodegenerative disorder characterized by dopaminergic neuronal loss and impaired motor and non-motor functions. Current therapies provide symptomatic relief but do not prevent disease progression, highlighting the need for novel neuroprotective agents. Cyanobacteria-derived bioactive compounds possess diverse pharmacological properties, including antioxidant and anti-inflammatory activities, making them potential therapeutic candidates. This study aimed to identify potential monoamine oxidase B (MAO-B) targeting cyanobacterial compounds using computational approaches. Methods: Forty-five cyanobacterial bioactive compounds were screened from the literature and databases based on their antioxidant and anti-inflammatory properties. Molecular docking against MAO-B was performed using PyRx, and interactions were analyzed using BIOVIA Discovery Studio. The top-ranked compounds were evaluated through ADMET profiling and 100 ns molecular dynamics simulations using the CHARMM36 force field with Swiss-Param-based ligand parameterization. Results: Among the screened compounds, Scytonemin (-11.9 kcal/mol), Calothrixin B (-11.7 kcal/mol), and Zeaxanthin (-11.5 kcal/mol) demonstrated the predicted binding affinity toward MAO-B. ADMET analysis revealed distinct pharmacokinetic profiles among the selected compounds. ADMET analysis showed favorable GI absorption and BBB permeability for Calothrixin B, whereas Scytonemin and Zeaxanthin showed low GI absorption and no predicted BBB permeability. Molecular dynamics simulations indicated stable ligand-protein interactions throughout the simulation period. Conclusions: The integrative computational analysis identified Scytonemin, Calothrixin B, and Zeaxanthin as potential MAO-B-interacting candidates. Based on the combined docking, ADMET, and molecular dynamics profiles, Calothrixin B demonstrated the most favorable overall computational profile, followed by Scytonemin and Zeaxanthin, highlighting all three compounds as candidates for further investigation in MAO-B-targeted drug discovery. Further in vitro and in vivo studies are required to validate their MAO-B inhibitory, neuroprotective, and pharmacokinetic potential. FAU - Khalid, Sarah AU - Khalid S AD - Department of Biosciences, Faculty of Science, Integral Centre of Excellence for Interdisciplinary Research (ICEIR), Integral University, Lucknow 226026, India. FAU - Ahmad, Suhail AU - Ahmad S AD - Department of Bioengineering, Integral University, Lucknow 226026, India. FAU - Kaushik, Aman Chandra AU - Kaushik AC AD - CSIR-Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow 226015, India. FAU - Sebaei, Mahmoud G El AU - Sebaei MGE AUID- ORCID: 0000-0002-2807-8710 AD - Department of Biomedical Sciences, College of Clinical Pharmacy, King Faisal University, Al Ahsa 31982, Saudi Arabia. FAU - Soliman, Wafaa E AU - Soliman WE AUID- ORCID: 0000-0001-6549-0926 AD - Department of Biomedical Sciences, College of Clinical Pharmacy, King Faisal University, Al Ahsa 31982, Saudi Arabia. FAU - Zaidi, Noushi AU - Zaidi N AD - Department of Chemistry, College of Science, King Faisal University, Al-Ahsa 31982, Saudi Arabia. FAU - Tripathi, Rina AU - Tripathi R AD - Department of Pharmacy Practice, College of Clinical Pharmacy, King Faisal University, Al Ahsa 31982, Saudi Arabia. FAU - Nafees, Sana AU - Nafees S AD - Department of Biomedical Sciences, College of Clinical Pharmacy, King Faisal University, Al Ahsa 31982, Saudi Arabia. FAU - Rumman, Mohammad AU - Rumman M AD - Department of Biosciences, Faculty of Science, Integral Centre of Excellence for Interdisciplinary Research (ICEIR), Integral University, Lucknow 226026, India. FAU - Yasin, Durdana AU - Yasin D AD - Department of Biosciences, Faculty of Science, Integral Centre of Excellence for Interdisciplinary Research (ICEIR), Integral University, Lucknow 226026, India. LA - eng PT - Journal Article DEP - 20261002 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708360 OTO - NOTNLM OT - Parkinson's disease OT - cyanobacteria OT - in silico drug discovery OT - molecular docking OT - molecular dynamics simulation OT - monoamine oxidase-B OT - neurodegeneration COIS- No potential conflicts of interest are reported by the authors. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/02 CRDT- 2026/10/27 10:44 PHST- 2026/07/31 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/02 00:00 [pmc-release] AID - ph19101569 [pii] AID - pharmaceuticals-19-01569 [pii] AID - 10.3390/ph19101569 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 2;19(10):1569. doi: 10.3390/ph19101569. PMID- 42943264 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 1 TI - Oxidative Susceptibility of Coumarin-1,2,3-Triazole-Isatin Hybrids: Integrating In Silico CYP-Mediated Metabolism Prediction with Electrochemistry-Mass Spectrometry. LID - 10.3390/ph19101568 [doi] LID - 1568 AB - Background/Objectives: Oxidative metabolism can influence the development of bioactive compounds by affecting metabolic stability and cytochrome P450 (CYP) interactions. This study aimed to assess the CYP-related properties and oxidative susceptibility of 21 coumarin-1,2,3-triazole-isatin hybrids by integrating in silico prediction with online electrochemistry-mass spectrometry (EC-MS). Methods: All 21 derivatives were evaluated in silico for CYP substrate and inhibitor profiles and CYP-mediated transformations. Four structurally representative derivatives (6a1, 6b1, 6c1, and 6c2) were selected for analysis by directly coupled online electrochemistry-mass spectrometry (EC-MS). Compounds 6a1, 6b1, and 6c1 formed a C2-C4 homologous series, whereas 6c2 was the 5-fluoro analogue of 6c1. Results: CYP3A4 was the isoform most consistently associated with substrate classification, while CYP3A4 inhibition probabilities remained close to the classification threshold. GLORY identified aromatic hydroxylation as the predominant top-ranked transformation across the series. Linker length had little influence on the principal predicted oxidation sites. EC-MS revealed a conserved principal oxidation pattern across the C2-C4 homologues, including +16 Da ions and two lower-mass ion series showing systematic 14 Da shifts with each additional methylene group, compatible with monooxygenation, oxidative ether cleavage, and N-dealkylation. In the 6c1/6c2 pair, 5-fluoro substitution removed the second-ranked isatin 5-position hydroxylation predicted for 6c1, leaving the three highest-ranked predictions for 6c2 on the coumarin ring, and was accompanied by a selective 18 Da shift in an isatin-containing electrochemical oxidation product. Conclusions: The combined in silico and EC-MS approach identified structurally dependent predicted CYP-related properties and electrochemical oxidative susceptibility within this series. The findings support further evaluation of 6c1 and related analogues using biological metabolism models to establish their metabolic stability and CYP-mediated transformation pathways. FAU - Dimkovski, Aleksandar AU - Dimkovski A AD - Institute for Pharmaceutical Chemistry, Faculty of Pharmacy, Ss Cyril and Methodius University in Skopje, Majka Tereza 47, 1000 Skopje, North Macedonia. FAU - Krzhovska, Darija AU - Krzhovska D AD - Institute for Pharmaceutical Chemistry, Faculty of Pharmacy, Ss Cyril and Methodius University in Skopje, Majka Tereza 47, 1000 Skopje, North Macedonia. FAU - Dobricic, Vladimir AU - Dobricic V AUID- ORCID: 0000-0002-5086-9017 AD - Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia. FAU - Simic, Milena R AU - Simic MR AD - Department of Organic Chemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia. FAU - Mihajloska, Evgenija AU - Mihajloska E AUID- ORCID: 0000-0001-8494-3644 AD - Institute for Pharmaceutical Chemistry, Faculty of Pharmacy, Ss Cyril and Methodius University in Skopje, Majka Tereza 47, 1000 Skopje, North Macedonia. FAU - Nestorovska, Aleksandra Kapedanovska AU - Nestorovska AK AUID- ORCID: 0000-0003-0931-5052 AD - Institute for Pharmaceutical Chemistry, Faculty of Pharmacy, Ss Cyril and Methodius University in Skopje, Majka Tereza 47, 1000 Skopje, North Macedonia. FAU - Grozdanova, Aleksandra AU - Grozdanova A AUID- ORCID: 0000-0002-0098-8691 AD - Institute for Pharmaceutical Chemistry, Faculty of Pharmacy, Ss Cyril and Methodius University in Skopje, Majka Tereza 47, 1000 Skopje, North Macedonia. FAU - Poceva Panovska, Ana AU - Poceva Panovska A AUID- ORCID: 0000-0001-9012-2447 AD - Institute for Applied Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy, Ss Cyril and Methodius University in Skopje, Majka Tereza 47, 1000 Skopje, North Macedonia. FAU - Sterjev, Zoran AU - Sterjev Z AD - Institute for Pharmaceutical Chemistry, Faculty of Pharmacy, Ss Cyril and Methodius University in Skopje, Majka Tereza 47, 1000 Skopje, North Macedonia. LA - eng GR - Project ID 719675/Ministry of Education and Science/ PT - Journal Article DEP - 20261001 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708526 OTO - NOTNLM OT - coumarin-1,2,3-triazole-isatin hybrids OT - cytochrome P450 OT - electrochemical oxidation OT - electrochemistry-mass spectrometry OT - in silico metabolism prediction OT - oxidative metabolism COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:44 PHST- 2026/09/09 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - ph19101568 [pii] AID - pharmaceuticals-19-01568 [pii] AID - 10.3390/ph19101568 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Oct 1;19(10):1568. doi: 10.3390/ph19101568. PMID- 42943262 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Oct 1 TI - Reduced Epithelial CD1a+ Langerhans Cell Density Is Associated with Advanced Stage and Nodal Positivity in Head and Neck Squamous Cell Carcinoma: A Cross-Sectional Immunohistochemical Study. LID - 10.3390/ph19101564 [doi] LID - 1564 AB - Background/Objectives: Chronic inflammation is increasingly recognized as a driver of head and neck squamous cell carcinoma (HNSCC) progression, but the interplay between local immune surveillance and angiolymphatic remodeling remains incompletely characterized. We hypothesized that advanced HNSCC stage would be associated with lower epithelial CD1a+ Langerhans cell density alongside higher proliferation, reduced E-cadherin-mediated adhesion, and higher angiogenic/lymphangiogenic activity, and aimed to relate this marker pattern to tumor stage defined by combined histopathological and radiological criteria. Methods: Fifty HNSCC specimens were included (mean age, 63.9 +/- 8.7 years; 86% male; 88% current or former smokers). Specimens were evaluated immunohistochemically for CD1a (primary Langerhans cell metric) and S100 (supportive marker), Ki-67, E-cadherin, VEGF, and Ki-67/D2-40 double staining (proliferating lymphatic vessel density (pLVD), reported descriptively only), alongside hematoxylin and eosin (H&E)-based histological grading and combined histopathological/radiological (CT/MRI) staging. Associations were assessed using Spearman correlation, Mann-Whitney U/chi-square tests, and multivariable logistic regression. Results: CD1a+ epithelial density, the primary Langerhans-cell metric, was significantly lower in advanced-stage and node-positive tumors (median 22.30 vs. 32.75 cells/mm(2), p = 0.0008; and 19.05 vs. 31.15 cells/mm(2), p < 0.0001, respectively), while the Ki-67 index and VEGF immunoreactive score were correspondingly higher (all p /=30% occurred more often with finerenone than with usual care (36% vs. 14%; odds ratio, 3.46; 95% CI, 1.29-9.26; p = 0.014). Changes in serum creatinine, eGFR, and serum potassium did not differ significantly between groups. In exploratory dose-stratified analyses, standard-dose finerenone produced a greater UACR reduction than no finerenone; reduced-dose regimens did not differ significantly from no finerenone, and the direct standard- versus reduced-dose comparison did not reach conventional significance. Conclusions: Finerenone initiation was associated with lower adjusted albuminuria at four weeks. Four-week UACR assessment may support early monitoring but is not a validated threshold for treatment continuation, discontinuation, or dose escalation. No short-term safety signal was identified, although the study was not powered to assess comparative or long-term safety. Larger prospective studies are needed to determine whether early UACR changes predict long-term cardiorenal benefit. FAU - Hsu, Jui-Ting AU - Hsu JT AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833401, Taiwan. FAU - Tseng, Pei-Chen AU - Tseng PC AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833401, Taiwan. FAU - Li, Lung-Chih AU - Li LC AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833401, Taiwan. FAU - Chiou, Terry Ting-Yu AU - Chiou TT AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833401, Taiwan. FAU - Chiu, Chien-Hua AU - Chiu CH AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833401, Taiwan. FAU - Kuo, Po-Yen AU - Kuo PY AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833401, Taiwan. FAU - Huang, Chiang-Chi AU - Huang CC AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833401, Taiwan. FAU - Tsai, Kai-Fan AU - Tsai KF AUID- ORCID: 0000-0002-0148-3302 AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833401, Taiwan. FAU - Hsu, Tsuen-Wei AU - Hsu TW AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833401, Taiwan. FAU - Wu, Po-Jung AU - Wu PJ AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833401, Taiwan. AD - Division of Nephrology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung 801735, Taiwan. FAU - Lee, Yueh-Ting AU - Lee YT AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833401, Taiwan. AD - Division of Nephrology, Kaohsiung Municipal Fong-Shan Hospital, Kaohsiung 830025, Taiwan. FAU - Lee, Chien-Te AU - Lee CT AUID- ORCID: 0000-0002-4704-1956 AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833401, Taiwan. AD - Division of Nephrology, Kaohsiung Municipal Fong-Shan Hospital, Kaohsiung 830025, Taiwan. FAU - Lee, Wen-Chin AU - Lee WC AUID- ORCID: 0000-0001-6172-1483 AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833401, Taiwan. FAU - Yang, Chih-Chao AU - Yang CC AUID- ORCID: 0000-0003-0049-5181 AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833401, Taiwan. AD - Division of Nephrology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung 801735, Taiwan. AD - Division of Nephrology, Kaohsiung Municipal Fong-Shan Hospital, Kaohsiung 830025, Taiwan. LA - eng PT - Journal Article DEP - 20260928 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708035 OTO - NOTNLM OT - albuminuria OT - diabetic kidney disease OT - dosing strategies OT - finerenone OT - non-steroidal mineralocorticoid receptor antagonist OT - real-world study COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/28 CRDT- 2026/10/27 10:44 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - ph19101539 [pii] AID - pharmaceuticals-19-01539 [pii] AID - 10.3390/ph19101539 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Sep 28;19(10):1539. doi: 10.3390/ph19101539. PMID- 42943235 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Sep 28 TI - Long-Term Drug Screening Identifies PARP Inhibitors as Irinotecan Combination Partners in BRCA1/2-Wild-Type Pancreatic Ductal Adenocarcinoma. LID - 10.3390/ph19101538 [doi] LID - 1538 AB - Background/Objectives: Irinotecan is a component of standard first-line regimens for advanced pancreatic ductal adenocarcinoma (PDAC), yet cells that survive irinotecan treatment may retain the capacity to re-establish growth. Most anticancer drug screens use short-term growth inhibition as an initial criterion for compound selection, potentially excluding compounds with limited acute activity. We asked whether some of these excluded candidates might nevertheless show substantial long-term activity and enhance irinotecan responses in combination. Methods: We first screened 3524 compounds for short-term growth inhibition and advanced compounds with limited acute activity to a 10-day colony-growth screen. Long-term hits were narrowed to clinically relevant anticancer agents and then screened with irinotecan. Talazoparib showed the lowest combination-to-single-agent score and was selected for further evaluation. The combination was evaluated across multiple BRCA1/2-wild-type PDAC cell lines and further validated using additional PARP inhibitors and a xenograft model. RNA sequencing and gammaH2AX analysis were used to examine associated DNA damage-response features. Results: Of the 1877 compounds advanced from the initial 3-day screen, 334 (17.8%) met the long-term hit criterion. In short- versus long-term comparisons, irinotecan plus talazoparib produced greater growth suppression and higher Bliss synergy scores in 10-day than in 72 h assays. Enhanced long-term responses were observed across five BRCA1/2-wild-type PDAC cell lines, and similar long-term effects were observed with three additional PARP inhibitors. The combination also showed synergistic antitumor activity in an MIA PaCa-2 subcutaneous xenograft model. Transcriptomic and gammaH2AX analyses provided findings consistent with replication-stress and DNA-damage-response processes. Conclusions: A substantial subset of compounds with limited acute activity showed substantial long-term activity. PARP inhibition enhanced irinotecan responses and emerged as a candidate combination strategy in BRCA1/2-wild-type PDAC. FAU - Jang, Hyeweon AU - Jang H AUID- ORCID: 0000-0003-3841-018X AD - Division of Cancer Biology, Research Institute, National Cancer Center, Goyang 10408, Republic of Korea. FAU - Jang, Hansol AU - Jang H AUID- ORCID: 0000-0003-0028-6435 AD - Division of Cancer Biology, Research Institute, National Cancer Center, Goyang 10408, Republic of Korea. FAU - Yun, Hye Yeon AU - Yun HY AUID- ORCID: 0009-0002-1410-5041 AD - Division of Cancer Biology, Research Institute, National Cancer Center, Goyang 10408, Republic of Korea. FAU - Lee, Kyung Yong AU - Lee KY AUID- ORCID: 0000-0003-2701-0762 AD - Division of Cancer Biology, Research Institute, National Cancer Center, Goyang 10408, Republic of Korea. AD - Department of Cancer Biomedical Science, National Cancer Center Graduate School of Cancer Science and Policy, Goyang 10408, Republic of Korea. FAU - Jang, Hyonchol AU - Jang H AUID- ORCID: 0000-0003-1436-457X AD - Division of Cancer Biology, Research Institute, National Cancer Center, Goyang 10408, Republic of Korea. AD - Department of Cancer Biomedical Science, National Cancer Center Graduate School of Cancer Science and Policy, Goyang 10408, Republic of Korea. LA - eng GR - 2022R1A2C2009720/National Research Foundation of Korea/ GR - 2022R1A5A102641311/National Research Foundation of Korea/ GR - NCC-2511570/National Cancer Center/ GR - NCC-2510732/National Cancer Center/ PT - Journal Article DEP - 20260928 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708644 OTO - NOTNLM OT - BRCA1/2-wild-type OT - PARP inhibitor OT - clonogenic assay OT - drug combination OT - drug screening OT - irinotecan OT - pancreatic ductal adenocarcinoma COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/28 CRDT- 2026/10/27 10:44 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - ph19101538 [pii] AID - pharmaceuticals-19-01538 [pii] AID - 10.3390/ph19101538 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Sep 28;19(10):1538. doi: 10.3390/ph19101538. PMID- 42943234 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Sep 28 TI - Multi-Target Virtual Screening of Natural Products Against Virulence-Associated Proteins of Pseudomonas aeruginosa PAO1 for Antivirulence Candidate Prioritization. LID - 10.3390/ph19101537 [doi] LID - 1537 AB - Background/Objectives: Natural products offer chemically diverse starting points for antivirulence discovery. We aimed to prioritize candidates against ten Pseudomonas aeruginosa PAO1 virulence-associated proteins while accounting for differences in structural support and compound-access requirements. Methods: We screened 391,658 COCONUT-derived ligand entries using staged AutoDock Vina docking, followed by GNINA rescoring and within-target Binding-Access-Exposure-Liability (BAEL) prioritization. Scaffold profiling, ranking sensitivity analyses, and triplicate 100 ns molecular dynamics simulations of 30 selected complexes characterized chemical diversity, selection consistency, and trajectory behavior. A separate retrospective LasB analysis examined sensitivity to comparison design. No prospective biochemical or cellular validation was performed. Results: The workflow yielded 189 candidate-target associations involving 182 unique natural products across six functional classes. The 181 scaffold-bearing compounds represented 176 Bemis-Murcko families. BAEL retained 179 of 200 Binding-only selections, with property-informed substitutions concentrated near the selection cutoffs. Top-20 selection overlap remained high under prespecified 25% perturbations of the Access, Exposure, and Liability rules (Jaccard similarity >/= 0.905). Of 90 trajectories, 82 were classified as pocket-proximal under the modeled conditions. In the LasB analysis, areas under the receiver operating characteristic curve were 0.700/0.740 for Vina/GNINA with surrogate controls and 0.480/0.480 in the attempted property-matching sensitivity set, which showed residual property imbalance and differed in annotated-parent coverage and weighting. Conclusions: This study connects large-scale natural-product screening with target-specific experimental planning. Binding and antivirulence activity remain to be established, but the annotated candidates provide a chemically diverse starting point for focused biochemical and cellular evaluation. FAU - Chen, Wenjie AU - Chen W AUID- ORCID: 0009-0001-9232-5691 AD - School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan 430205, China. FAU - Zhang, Yu AU - Zhang Y AD - School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan 430205, China. FAU - Zeng, Jia AU - Zeng J AD - School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan 430205, China. FAU - Wang, Yuan AU - Wang Y AD - School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan 430205, China. FAU - Cheng, Bo AU - Cheng B AD - School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan 430205, China. AD - Hubei Key Laboratory of Microbial Transformation and Regulation of Biogenic Elements in the Middle Reaches of the Yangtze River, School of Environmental Ecology and Biological Engineering, Wuhan Institute of Technology, Wuhan 430205, China. LA - eng GR - A25-1-034/Yichang Science and Technology R&D Project/ PT - Journal Article DEP - 20260928 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708683 OTO - NOTNLM OT - Pseudomonas aeruginosa OT - antivirulence candidate prioritization OT - control-set sensitivity OT - molecular dynamics OT - multi-target virtual screening OT - natural products COIS- The authors declare no conflicts of interest. The funder provided financial support only and had no role in study design, data collection, analysis or interpretation, manuscript preparation, or the decision to publish. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/28 CRDT- 2026/10/27 10:44 PHST- 2026/08/22 00:00 [received] PHST- 2026/09/10 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - ph19101537 [pii] AID - pharmaceuticals-19-01537 [pii] AID - 10.3390/ph19101537 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Sep 28;19(10):1537. doi: 10.3390/ph19101537. PMID- 42943233 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Sep 28 TI - Biological Effects of Umbelliferone in MCF-7 Breast Cancer Cells: Integrated In Vitro and Computational Analyses. LID - 10.3390/ph19101536 [doi] LID - 1536 AB - Background/Objectives: Breast cancer remains a leading cause of cancer-related mortality among women, highlighting the need for further investigation of bioactive compounds with selective effects on cancer cells. This study evaluated the biological effects of the natural coumarin derivative umbelliferone in MCF-7 breast cancer cells using integrated in silico and in vitro approaches. Methods: Molecular docking predicted interactions with AKT1, mTOR, PI3Kalpha, and Caspase-7, while molecular dynamics simulations and MM/PBSA calculations provided further information on interactions with AKT1 and mTOR. Results: Umbelliferone reduced MCF-7 cell viability in a concentration-dependent manner, with an IC(50) of 492 microM after 24 h, whereas 50% viability was not reached in BJ normal fibroblasts up to 1400 microM, corresponding to an IC(50) > 1400 microM and a selectivity index > 2.85. Annexin V-FITC/PI analysis showed increased apoptotic populations, with total apoptosis significantly increased at 800 microM. Colony formation analysis further indicated reduced clonogenic capacity. At 400 microM, umbelliferone significantly increased relative Caspase-3/7 and Caspase-9 activities to 1.873 +/- 0.635 and 2.483 +/- 0.092, respectively, whereas neither activity was significantly increased at 800 microM. In silico ADME analysis predicted properties compatible with commonly used drug-likeness criteria. Conclusions: Collectively, the findings demonstrate effects of umbelliferone on cell viability, apoptosis, clonogenic capacity, and caspase activity in MCF-7 cells, with comparatively lower cytotoxicity toward normal fibroblasts. Computational findings remain hypothesis-generating and do not establish direct target engagement or pathway inhibition. Further mechanistic and in vivo studies are required to determine the biological and pharmacological relevance of these findings. FAU - Caliskan, Serife Gokce AU - Caliskan SG AUID- ORCID: 0000-0001-5421-3472 AD - Department of Physics, Faculty of Sciences, Aydin Adnan Menderes University, 09010 Aydin, Turkiye. LA - eng PT - Journal Article DEP - 20260928 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708397 OTO - NOTNLM OT - ADME OT - MCF-7 OT - apoptosis OT - breast cancer OT - caspase activity OT - molecular docking OT - molecular dynamics OT - umbelliferone COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/28 CRDT- 2026/10/27 10:44 PHST- 2026/09/09 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - ph19101536 [pii] AID - pharmaceuticals-19-01536 [pii] AID - 10.3390/ph19101536 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Sep 28;19(10):1536. doi: 10.3390/ph19101536. PMID- 42943232 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Sep 28 TI - When Does Magnesium Stearate Improve Carrier-Based Dry Powder Inhalers (DPI) Performance? A Critical Review of Material, Process, and Formulation Factors. LID - 10.3390/ph19101535 [doi] LID - 1535 AB - Magnesium stearate (MgSt), although well established as a pharmaceutical lubricant in solid oral dosage forms, plays a more complex role in carrier-based dry powder inhalers (DPIs), where it functions as a surface modifier and force control agent (FCA). In DPI formulations, MgSt can influence powder rheology, moisture resistance, interparticulate interactions, and aerosolization efficiency; however, these effects depend not simply on its presence or concentration, but on whether effective surface modification is achieved. This review critically evaluates experimental evidence on MgSt-mediated particle coating in carrier-based DPIs, with particular emphasis on how material attributes, particle cohesion and surface area, process energy, coating strategy, and MgSt concentration interact to determine coating efficiency and formulation performance. By integrating these factors, the review aims to provide a mechanistic framework for selecting and optimizing MgSt coating strategies and to identify methodological priorities for future research. The available evidence indicates that process energy is a major determinant of coating efficiency. High-energy techniques, particularly mechanofusion, can effectively modify highly cohesive micronized particles that are difficult to coat using conventional blending, whereas high-shear blending may provide sufficient energy for surface modification of larger, coarse lactose carriers. Mechanical milling represents a distinct strategy in which particle-size reduction and surface modification occur simultaneously. Direct comparison between coating approaches remains difficult because studies differ in MgSt grade, carrier and API properties, particle size distribution, processing conditions, and methods used to assess coating efficiency. MgSt generally improves aerosolization by modifying cohesive and adhesive interactions, but the magnitude of this effect is formulation-specific. Improvements in fine-particle delivery depend on MgSt particle size and specific surface area, the properties and surface area of the particles being coated, process energy, and the resulting coating efficiency. Consequently, increasing MgSt concentration does not necessarily improve performance, and excessive amounts may lead to overlubrication or MgSt agglomeration. Despite considerable progress, important knowledge gaps remain regarding how MgSt coating translates into improved DPI performance. Further studies integrating material properties, processing conditions, surface modification, and formulation performance are needed to establish more robust relationships between coating strategy and functional outcomes. Such knowledge may support more rational and formulation-specific use of MgSt in carrier-based DPIs. FAU - Rzewinska-Dabrowska, Aleksandra AU - Rzewinska-Dabrowska A AUID- ORCID: 0000-0002-4809-6377 AD - Department of Drug Technology and Pharmaceutical Biotechnology, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warszawa, Poland. AD - R&D Center, LEK-AM., Ostrzykowizna 14A, 05-170 Zakroczym, Poland. FAU - Wojcik, Katarzyna AU - Wojcik K AD - R&D Center, LEK-AM., Ostrzykowizna 14A, 05-170 Zakroczym, Poland. FAU - Juszczyk, Ewelina AU - Juszczyk E AUID- ORCID: 0000-0001-5629-5635 AD - R&D Center, Celon Pharma S.A., Marymoncka 15, 05-052 Kazun Nowy, Poland. FAU - Tratkiewicz, Ewa AU - Tratkiewicz E AD - R&D Center, LEK-AM., Ostrzykowizna 14A, 05-170 Zakroczym, Poland. FAU - Dorozynski, Przemyslaw AU - Dorozynski P AUID- ORCID: 0000-0001-6835-2916 AD - Department of Drug Technology and Pharmaceutical Biotechnology, Faculty of Pharmacy, Medical University of Warsaw, Banacha 1, 02-097 Warszawa, Poland. AD - Department of Inorganic and Analytical Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 Krakow, Poland. LA - eng GR - DWD/6/0500/2022/Ministry of Science and Higher Education/ PT - Journal Article PT - Review DEP - 20260928 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708093 OTO - NOTNLM OT - carrier-based DPI OT - fine particle dose OT - force control agent OT - magnesium stearate COIS- Authors Aleksandra Rzewinska-Dabrowska, Katarzyna Wojcik, Ewelina Juszczyk and Ewa Tratkiewicz were employed by the company R&D Center. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/28 CRDT- 2026/10/27 10:44 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - ph19101535 [pii] AID - pharmaceuticals-19-01535 [pii] AID - 10.3390/ph19101535 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Sep 28;19(10):1535. doi: 10.3390/ph19101535. PMID- 42943227 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Sep 27 TI - Structural Compatibility and Dynamic Behaviour of Elsholtzia Ketones in the CCT128930-Defined TRPM7 Vanilloid-like Region. LID - 10.3390/ph19101530 [doi] LID - 1530 AB - Background/Objectives: Transient receptor potential melastatin 7 (TRPM7) is a multifunctional ion channel-kinase containing a structurally resolved vanilloid-like (VL) regulatory region. Elsholtzia ketone (EK) and dehydroelsholtzia ketone (DEK), major ketones of Elsholtzia ciliata essential oil, are associated with cardiovascular effects, but their molecular target spectrum remains incompletely defined. We investigated whether EK and DEK can access and maintain structurally plausible configurations within the CCT128930-defined TRPM7 VL region. Methods: The 2.45 A cryo-EM structure of mouse TRPM7 bound to CCT128930 (PDB 8W2L; M05) was prepared in an explicit lipid membrane and subjected to restrained minimization. The workflow combined repeated M05 redocking, blind and focused docking, RICS-based within-ligand pose selection, pose clustering, property-matched decoy controls, and 100-ns explicit-membrane molecular dynamics (MD). Results: Minimization preserved the VL architecture and all 12 M05 reference contacts. Ten independent M05 redocking runs recovered native-like poses in every run (best direct heavy-atom RMSD, 0.567 A), although these ranked only 8-10 by Vina energy. Blind docking independently sampled the VL region for M05, EK, and DEK. Focused docking identified recurrent EK and DEK pose families reproducing 10/12 M05 contacts (Jaccard = 0.833). Property-matched decoys reached comparable high-end contact similarity, showing that reference compatibility was not ligand-specific. During MD, M05 showed the strongest positional and contact retention, EK intermediate redistribution, and DEK greater copy-dependent heterogeneity. Conclusions: EK and DEK can adopt M05-compatible geometries within the TRPM7 VL region, providing experimentally testable structural hypotheses. However, docking similarity and RICS do not demonstrate affinity, selectivity, potency, or functional TRPM7 modulation. FAU - Jurevicius, Jonas AU - Jurevicius J AD - Laboratory of Membrane Biophysics, Institute of Cardiology, Lithuanian University of Health Sciences, Sukileliu Ave. 15, LT50162 Kaunas, Lithuania. FAU - Martisiene, Irma AU - Martisiene I AUID- ORCID: 0009-0007-5613-4293 AD - Laboratory of Membrane Biophysics, Institute of Cardiology, Lithuanian University of Health Sciences, Sukileliu Ave. 15, LT50162 Kaunas, Lithuania. FAU - Almanaityte, Mante AU - Almanaityte M AD - Laboratory of Membrane Biophysics, Institute of Cardiology, Lithuanian University of Health Sciences, Sukileliu Ave. 15, LT50162 Kaunas, Lithuania. FAU - Andriule, Inga AU - Andriule I AD - Laboratory of Membrane Biophysics, Institute of Cardiology, Lithuanian University of Health Sciences, Sukileliu Ave. 15, LT50162 Kaunas, Lithuania. FAU - Macianskiene, Regina AU - Macianskiene R AUID- ORCID: 0000-0003-1979-9425 AD - Laboratory of Membrane Biophysics, Institute of Cardiology, Lithuanian University of Health Sciences, Sukileliu Ave. 15, LT50162 Kaunas, Lithuania. LA - eng PT - Journal Article DEP - 20260927 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708375 OTO - NOTNLM OT - CCT128930 OT - Elsholtzia ciliata OT - Elsholtzia ketone OT - TRPM7 OT - dehydroelsholtzia ketone OT - molecular docking OT - molecular dynamics OT - vanilloid-like region COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/27 CRDT- 2026/10/27 10:44 PHST- 2026/08/25 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - ph19101530 [pii] AID - pharmaceuticals-19-01530 [pii] AID - 10.3390/ph19101530 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Sep 27;19(10):1530. doi: 10.3390/ph19101530. PMID- 42943226 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Sep 26 TI - Drug Utilization Patterns and Potential Drug-Drug Interaction Burden in Respiratory Prescriptions: A Real-World Community Pharmacy Study. LID - 10.3390/ph19101528 [doi] LID - 1528 AB - Background: Respiratory disorders are frequently managed in community pharmacies and may involve complex multidrug regimens, particularly in asthma and chronic obstructive pulmonary disease (COPD). We aimed to characterize respiratory pharmacotherapy and moderate or major potential drug-drug interaction (DDI) risk using one index respiratory prescription per patient. Methods: We performed a retrospective observational secondary analysis of anonymized electronic prescription data from two Romanian community pharmacies (November 2022-October 2023). From a combined source dataset comprising prescriptions with at least two active substances, we selected records containing at least one eligible respiratory diagnosis. Demographics, diagnostic groups, active substances, therapeutic classes, medication burden, and potential DDIs were evaluated. Multivariable logistic regression identified factors independently associated with at least one moderate or major potential DDI. Results: The final cohort included 643 patients. Median age was 52 years [interquartile range 23.5-66.0], and 50.7% were female. Asthma was the most frequent diagnostic group (41.1%), followed by acute upper respiratory tract infections (19.8%) and COPD (15.4%). The most frequent therapeutic classes were inhaled and intranasal corticosteroids (54.0%), long-acting beta-2 agonists (46.7%), and anti-infectives (35.0%). At least one moderate or major potential DDI was identified in 28.0% of index prescriptions. In the fully adjusted multivariable model, each additional active substance was associated with higher potential DDI odds (odds ratio [OR] 2.42, 95% confidence interval [CI] 1.96-3.00), while cardiovascular or metabolic co-medication showed the strongest positive class-level association (OR 14.16, 95% CI 5.25-38.21). Conclusions: Respiratory prescriptions showed distinct diagnostic and therapeutic profiles. The highest prevalence of moderate or major potential DDIs among the major diagnostic groups was observed in COPD. Potential DDI risk was strongly associated with medication burden and cardiovascular/metabolic co-medication, supporting targeted community pharmacy medication review that considers both respiratory therapy and the broader pharmacotherapeutic regimen. FAU - Dragoi, Maria-Medana AU - Dragoi MM AUID- ORCID: 0009-0004-4064-5871 AD - Center for Drug Data Analysis, Cheminformatics, and the Internet of Medical Things, "Victor Babes" University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania. AD - Doctoral School of Pharmacy, "Victor Babes" University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania. FAU - Chis, Aimee Rodica AU - Chis AR AUID- ORCID: 0000-0001-7184-388X AD - Center for Drug Data Analysis, Cheminformatics, and the Internet of Medical Things, "Victor Babes" University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania. AD - Department of Biochemistry, "Victor Babes" University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania. AD - Center for Complex Network Science, "Victor Babes" University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania. FAU - Ardelean, Sebastian-Mihai AU - Ardelean SM AUID- ORCID: 0000-0003-0968-1191 AD - Department of Computer and Information Technology, Politehnica University Timisoara, 300223 Timisoara, Romania. FAU - Udrescu, Mihai AU - Udrescu M AUID- ORCID: 0000-0002-7607-9240 AD - Department of Computer and Information Technology, Politehnica University Timisoara, 300223 Timisoara, Romania. FAU - Suciu, Liana AU - Suciu L AUID- ORCID: 0000-0002-2075-3275 AD - Center for Drug Data Analysis, Cheminformatics, and the Internet of Medical Things, "Victor Babes" University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania. AD - Department II-Pharmacology, Physiology and Physiopathology, "Victor Babes" University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania. AD - Research Center for Experimental Pharmacology and Drug Design (X-Pharm Design), "Victor Babes" University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania. FAU - Udrescu, Lucretia AU - Udrescu L AUID- ORCID: 0000-0002-3084-6301 AD - Center for Drug Data Analysis, Cheminformatics, and the Internet of Medical Things, "Victor Babes" University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania. AD - Department I-Clinical Pharmacy and Drug Analysis, "Victor Babes" University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania. LA - eng PT - Journal Article DEP - 20260926 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708138 OTO - NOTNLM OT - community pharmacy OT - drug utilization OT - drug-drug interactions OT - medication safety OT - polypharmacy OT - real-world data OT - respiratory diseases COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/26 CRDT- 2026/10/27 10:44 PHST- 2026/08/12 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/09/26 00:00 [pmc-release] AID - ph19101528 [pii] AID - pharmaceuticals-19-01528 [pii] AID - 10.3390/ph19101528 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Sep 26;19(10):1528. doi: 10.3390/ph19101528. PMID- 42943218 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Sep 25 TI - RQ18 Peptide Exerts Anti-Melanoma Effects via Apoptosis Induction. LID - 10.3390/ph19101521 [doi] LID - 1521 AB - Background/Objectives: The rise in cancer cases is a major global public health problem. Treating the disease and maintaining health generate high economic and social costs. In recent years, treatment resistance has become a challenge, reinforcing the need for new anticancer agents. In this context, anticancer peptides (ACPs) stand out for their selective interaction with cancer cell membranes. This study aimed to evaluate the activity of the RQ18 peptide against cancer cells. Methods: The cytotoxicity of RQ18 was determined by the MTT assay in murine melanoma (B16F10-Nex2), human melanoma (Sk-mel-28 and A375), breast cancer (MCF-7), cervical cancer (HeLa), murine macrophage (RAW 264.7), and human fibroblast (FN1). Caspase-3/7 activity was further evaluated in B16F10-Nex2, Sk-mel-28, and A375 melanoma cells to investigate the involvement of apoptotic pathways. In addition, intracellular oxidative stress was assessed using the DCFH-DA probe in these three melanoma cell lines. In B16F10-Nex2 cells, cellular morphological changes were examined by microscopy, while apoptosis-associated events were characterized using the markers MitoTracker Deep Red, and NucBlue, complemented by flow cytometry analyses. In addition, molecular dynamics simulations were employed to investigate its interactions with lipid membranes. Results: The peptide exhibited anticancer activity, with IC(50) values of 4.4, 11.58 and 59.99 microM.mL(-1), against cells B16F10-Nex2, Sk-mel-28 and A375, respectively. Compared with non-cancerous cells, RQ18 preferentially affected cancer cells, especially B16F10-Nex2. In contrast, the peptide was nontoxic to healthy cells, thus indicating selectivity for cancer cells. Morphological analysis revealed alterations compatible with apoptosis, including cell rounding and chromatin condensation. RQ18 increased caspase-3/7 activity in B16F10-Nex2, Sk-mel-28, and A375 cells and induced intracellular oxidative stress, as indicated by increased DCFH-DA fluorescence. Flow cytometry data confirmed the induction of apoptotic cell death in B16F10-Nex2 cells. Caspase activation, detected by a caspase-3/7 assay in three melanoma cell lines, together with flow cytometry data, supported the involvement of apoptotic cell death. Molecular dynamics simulations suggest that the peptide interacts stably with cancer cell-like membranes and retains an alpha-helical conformation during membrane association. Conclusions: In summary, the RQ18 peptide demonstrates preferential anticancer activity, particularly against melanoma cells, supporting further investigation of its therapeutic potential. FAU - Almeida, Claudiane Vilharroel AU - Almeida CV AUID- ORCID: 0000-0002-9625-5766 AD - Programa de Pos-Graduacao em Bioquimica e Biologia Molecular, Instituto de Biociencias (INBIO), Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil. AD - Laboratorio de Purificacao de Proteinas e suas Funcoes Biologicas (LPPFB), Faculdade de Ciencias Farmaceuticas, Alimentos e Nutricao (FACFAN), Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil. FAU - Jacobowski, Ana Cristina AU - Jacobowski AC AUID- ORCID: 0000-0001-8233-0713 AD - Laboratorio de Purificacao de Proteinas e suas Funcoes Biologicas (LPPFB), Faculdade de Ciencias Farmaceuticas, Alimentos e Nutricao (FACFAN), Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil. FAU - Oliveira, Caio Fernando Ramalho de AU - Oliveira CFR AUID- ORCID: 0000-0001-9549-4848 AD - Laboratorio de Purificacao de Proteinas e suas Funcoes Biologicas (LPPFB), Faculdade de Ciencias Farmaceuticas, Alimentos e Nutricao (FACFAN), Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil. FAU - Almeida, Luis Henrique de Oliveira AU - Almeida LHO AUID- ORCID: 0000-0002-8322-1372 AD - Programa de Pos-Graduacao em Bioquimica e Biologia Molecular, Instituto de Biociencias (INBIO), Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil. AD - Laboratorio de Purificacao de Proteinas e suas Funcoes Biologicas (LPPFB), Faculdade de Ciencias Farmaceuticas, Alimentos e Nutricao (FACFAN), Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil. FAU - Gutierrez, Camila de Oliveira AU - Gutierrez CO AD - Laboratorio de Purificacao de Proteinas e suas Funcoes Biologicas (LPPFB), Faculdade de Ciencias Farmaceuticas, Alimentos e Nutricao (FACFAN), Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil. FAU - Monteiro-Alfredo, Tamaeh AU - Monteiro-Alfredo T AUID- ORCID: 0000-0001-5514-0797 AD - Laboratorio de Purificacao de Proteinas e suas Funcoes Biologicas (LPPFB), Faculdade de Ciencias Farmaceuticas, Alimentos e Nutricao (FACFAN), Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil. FAU - Lima, Lucas Rodrigues de AU - Lima LR AUID- ORCID: 0000-0001-8351-2551 AD - S-Inova Biotech, Programa de Pos-Graduacao em Biotecnologia, Universidade Catolica Dom Bosco, Campo Grande 79117-900, MS, Brazil. FAU - Rodrigues, Thais de Andrade Farias AU - Rodrigues TAF AUID- ORCID: 0000-0002-1937-0162 AD - Centro de Estudos em Celulas Tronco, Terapia Celular e Genetica Toxicologica (CeTroGen), Faculdade de Medicina (FAMED), Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil. FAU - Oliveira, Rodrigo Juliano AU - Oliveira RJ AUID- ORCID: 0000-0003-3514-3346 AD - Centro de Estudos em Celulas Tronco, Terapia Celular e Genetica Toxicologica (CeTroGen), Faculdade de Medicina (FAMED), Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil. FAU - Franco, Octavio Luiz AU - Franco OL AUID- ORCID: 0000-0001-9546-0525 AD - S-Inova Biotech, Programa de Pos-Graduacao em Biotecnologia, Universidade Catolica Dom Bosco, Campo Grande 79117-900, MS, Brazil. AD - Centro de Analises Proteomicas e Bioquimicas, Programa de Pos-Graduacao em Ciencias Genomicas e Biotecnologia, Universidade Catolica de Brasilia, Brasilia 71966-700, DF, Brazil. FAU - Cardoso, Marlon Henrique AU - Cardoso MH AUID- ORCID: 0000-0001-6676-5362 AD - Programa de Pos-Graduacao em Bioquimica e Biologia Molecular, Instituto de Biociencias (INBIO), Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil. AD - S-Inova Biotech, Programa de Pos-Graduacao em Biotecnologia, Universidade Catolica Dom Bosco, Campo Grande 79117-900, MS, Brazil. AD - Programa de Pos-Graduacao em Ciencias Ambientais e Sustentabilidade Agropecuaria, Universidade Catolica Dom Bosco, Campo Grande 79117-900, MS, Brazil. FAU - Boleti, Ana Paula de Araujo AU - Boleti APA AD - Laboratorio de Purificacao de Proteinas e suas Funcoes Biologicas (LPPFB), Faculdade de Ciencias Farmaceuticas, Alimentos e Nutricao (FACFAN), Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil. FAU - Macedo, Maria Ligia Rodrigues AU - Macedo MLR AUID- ORCID: 0000-0001-6969-6307 AD - Programa de Pos-Graduacao em Bioquimica e Biologia Molecular, Instituto de Biociencias (INBIO), Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil. AD - Laboratorio de Purificacao de Proteinas e suas Funcoes Biologicas (LPPFB), Faculdade de Ciencias Farmaceuticas, Alimentos e Nutricao (FACFAN), Universidade Federal de Mato Grosso do Sul, Campo Grande 79070-900, MS, Brazil. LA - eng GR - 314858/2020-2/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ GR - 01.23.0679.00/Financiadora de Estudos e Projetos/ GR - 71/048.583/2021/Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia do Estado de Mato Grosso do Sul/ GR - 71/039.178/2022/Fundacao de Apoio ao Desenvolvimento do Ensino, Ciencia e Tecnologia do Estado de Mato Grosso do Sul/ PT - Journal Article DEP - 20260925 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708664 OTO - NOTNLM OT - anticancer peptide OT - apoptosis OT - melanoma OT - molecular dynamics OT - selectivity cytotoxicity COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/25 CRDT- 2026/10/27 10:44 PHST- 2026/06/03 00:00 [received] PHST- 2026/09/02 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - ph19101521 [pii] AID - pharmaceuticals-19-01521 [pii] AID - 10.3390/ph19101521 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Sep 25;19(10):1521. doi: 10.3390/ph19101521. PMID- 42943217 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Sep 25 TI - Tissue Transglutaminase (TG2) as a Candidate Biomarker and Potential Therapeutic Target in Endometriosis: Functional Evidence from shRNA-Mediated Downregulation of TG2 in Endometrial Mesenchymal Stem Cells. LID - 10.3390/ph19101520 [doi] LID - 1520 AB - Background/Objectives: Endometriosis involves the presence of endometrial tissue outside the uterus. Since the migration of endometrial cells is pivotal to lesion development, tissue transglutaminase (TG2)-known for its roles in cell adhesion, migration, and stemness-may play a significant role. This study aimed to investigate the involvement of TG2 in the migratory and invasive characteristics of endometrial mesenchymal stem cells (eMSCs) derived from healthy individuals and endometriosis patients. Methods: eMSCs were isolated from healthy controls (heMSCs) and endometriosis patients (peMSCs). TG2 protein expression and enzyme activity were evaluated. Co-immunoprecipitation (Co-IP) experiments were performed to assess TG2 associations with ITGbeta-1 and SDC-4 in membrane fractions. Additionally, shRNA technology was utilized to silence TG2 in peMSCs to analyze its effects on cell proliferation, migration, invasion, and matrix metalloproteinase (MMP-2 and -9) activities. Results: peMSCs exhibited higher TG2 protein expression but lower enzyme activity compared to heMSCs. Co-IP analysis of membrane fractions revealed enhanced association of TG2 with ITGbeta-1 and SDC-4 in peMSCs, thereby promoting enhanced cell survival, migration, and invasive properties. Silencing TG2 in peMSCs via shRNA eliminated these enhanced proliferation, migration, and invasion capabilities, reverting them to a phenotype resembling heMSCs. Furthermore, elevated MMP-2 and -9 activities returned to basal levels following TG2 knockdown. Conclusions: These findings suggest that TG2 acts as a distinctive cell adhesion molecule directly involved in endometriosis pathogenesis. Collectively, these findings identify TG2 as a disease-associated molecular marker warranting further evaluation for its diagnostic potential and suggest that targeting its cell-adhesion functions may represent a novel therapeutic strategy for endometriosis. FAU - Kurt-Celep, Inci AU - Kurt-Celep I AUID- ORCID: 0000-0003-2459-2058 AD - Department of Genetics and Bioengineering, Yeditepe University, 26 August Campus, Kayisdagi, Atasehir, Istanbul 34755, Turkiye. AD - Department of Biotechnology, Faculty of Pharmacy, Istanbul Okan University, Tuzla, Istanbul 34959, Turkiye. FAU - Batukan, Melike AU - Batukan M AD - Department of Genetics and Bioengineering, Yeditepe University, 26 August Campus, Kayisdagi, Atasehir, Istanbul 34755, Turkiye. FAU - Sahin, Fikrettin AU - Sahin F AD - Department of Genetics and Bioengineering, Yeditepe University, 26 August Campus, Kayisdagi, Atasehir, Istanbul 34755, Turkiye. FAU - Telci, Dilek AU - Telci D AUID- ORCID: 0000-0002-2793-6533 AD - Department of Genetics and Bioengineering, Yeditepe University, 26 August Campus, Kayisdagi, Atasehir, Istanbul 34755, Turkiye. LA - eng PT - Journal Article DEP - 20260925 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708216 OTO - NOTNLM OT - TG2-ITGbeta-1/SDC-4 associations OT - cell cycle progression OT - endometrial mesenchymal stem cells (eMSCs) OT - endometriosis OT - matrix metalloproteinases OT - shRNA-mediated knockdown OT - tissue transglutaminase (TG2) COIS- The authors declare no other conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/25 CRDT- 2026/10/27 10:44 PHST- 2026/07/24 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - ph19101520 [pii] AID - pharmaceuticals-19-01520 [pii] AID - 10.3390/ph19101520 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Sep 25;19(10):1520. doi: 10.3390/ph19101520. PMID- 42943213 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Sep 24 TI - In Vitro Antileishmanial Activity and Cytotoxicity Evaluation of 2-(Thiophen-2-ylmethyl)quinazolin-4(3H)-one Derivatives Against Leishmania tropica and Leishmania infantum. LID - 10.3390/ph19101516 [doi] LID - 1516 AB - Background/Objectives: Leishmaniasis is a neglected tropical disease and the toxicity, limited efficacy and emerging drug resistance of current therapies underscore the urgent need for new antileishmanial agents. This study aimed to experimentally assess the in vitro antileishmanial activity, cytotoxicity and selectivity of quinazolin-4(3H)-one derivatives against Leishmania tropica and Leishmania infantum and to computationally investigate the potential interaction of compound E9 with trypanothione reductase. Methods: The in vitro antileishmanial activities of sixteen quinazolin-4(3H)-one derivatives (E1-E16) against the promastigote forms of L. tropica and L. infantum were evaluated. Additionally, the most active compounds were subjected to an evaluation of their cytotoxicity in human umbilical vein endothelial cells (HUVECs) and their selectivity indices (SIs) were subsequently calculated. Molecular docking and molecular mechanics/generalized Born surface area (MM/GBSA) analyses were performed for compound E9 against L. infantum trypanothione reductase. Results: Compounds E2, E9 and E11 showed activity against L. tropica, with half-maximal inhibitory concentration (IC(50)) values of 23.2, 22.4 and 76.0 muM, respectively. In contrast, compounds E2, E3, E9 and E11 were active against L. infantum, with IC(50) values of 38.1, 69.7, 28.2 and 86.7 muM, respectively, suggesting species-dependent activity. Furthermore, among the tested compounds, E2 showed the most favorable selectivity profile against L. tropica and L. infantum, with SI values of 8.8 and 5.4, respectively. Moreover, molecular docking of E9 produced a docking score of -8.070 kcal/mol, with MM/GBSA analysis indicating a binding free energy of -82.44 kcal/mol. Conclusions: These findings support further investigation of quinazolin-4(3H)-one derivatives as potential antileishmanial agents, with E2 emerging as the most selective compound. Furthermore, they suggest that the biological activity of this compound series may differ among Leishmania species. The molecular modeling findings suggest a potential interaction between E9 and trypanothione reductase, supporting the need for further evaluation of these compounds in intracellular amastigote models and subsequent in vivo studies. FAU - Karabag, Atilla AU - Karabag A AD - Department of Biology, Graduate School of Natural and Applied Sciences, Kafkas University, 36100 Kars, Turkiye. FAU - Mor, Baycan AU - Mor B AUID- ORCID: 0000-0001-9480-3143 AD - Department of Molecular Biology and Genetics, Faculty of Sciences and Letters, Kafkas University, 36100 Kars, Turkiye. LA - eng GR - 2025-FM-116/The Scientific Research Projects Coordination Unit of Kafkas University/ PT - Journal Article DEP - 20260924 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708542 OTO - NOTNLM OT - Leishmania infantum OT - Leishmania tropica OT - antileishmanial activity OT - molecular docking OT - quinazolin-4(3H)-one OT - selectivity index COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:44 PHST- 2026/08/28 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - ph19101516 [pii] AID - pharmaceuticals-19-01516 [pii] AID - 10.3390/ph19101516 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Sep 24;19(10):1516. doi: 10.3390/ph19101516. PMID- 42943204 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Sep 22 TI - Natural Modulators of the PPARgamma-Adiponectin Axis in Insulin Resistance: Structural Pharmacology, Receptor-State Selectivity, and Translational Readiness. LID - 10.3390/ph19101507 [doi] LID - 1507 AB - Insulin resistance reflects maladaptive communication among adipose tissue, the liver, skeletal muscle, immune cells, and the vasculature. Peroxisome proliferator-activated receptor gamma (PPARgamma) is a validated insulin-sensitizing target, but classical full agonism couples metabolic efficacy to weight gain, fluid retention, and skeletal liability. This critical narrative review evaluates plant-derived and dietary molecules through a receptor-state framework that distinguishes direct binding from reporter activation and separates full agonism, partial agonism, selective modulation, antagonism with retained metabolic function, and post-translational control of PPARgamma. Adiponectin is considered a functional inter-organ circuit linking adipocyte PPARgamma activity to AdipoR1/AdipoR2 signaling, AMPK, PPARalpha, ceramide metabolism, glucose disposal, and fatty-acid oxidation. Amorfrutins provide the most coherent target-led natural-product evidence, whereas chelerythrine offers a structurally defined selective-modulation benchmark; honokiol, diosmin, betulinic acid, valerenic acid, punicic acid, alkamides, and defined extracts show heterogeneous mechanistic and translational maturity. Human evidence linking natural PPARgamma ligands to verified target engagement and improved insulin sensitivity remains limited. We therefore propose a seven-stage translational roadmap connecting chemical identity, orthogonal binding, receptor-state pharmacology, cellular function, tissue exposure, mechanism-matched safety, and randomized human proof. FAU - Floriana Vasilica, Pirscoveanu Denisa AU - Floriana Vasilica PD AD - Department of Neurology, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Trasca, Diana-Maria AU - Trasca DM AD - Department of Internal Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Kamal, Adina Maria AU - Kamal AM AD - Department of Internal Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Varut, Renata Maria AU - Varut RM AD - Research Methodology Department, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. AD - Drug Research Center, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Popa, Romeo AU - Popa R AD - Department of Pharmacology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Dorin, Pluta Ion AU - Dorin PI AD - Faculty of Medical and Behavioral Sciences, Constantin Brancusi University of Targu Jiu, 210185 Targu Jiu, Romania. FAU - Rodica, Dirnu AU - Rodica D AD - Faculty of Medical and Behavioral Sciences, Constantin Brancusi University of Targu Jiu, 210185 Targu Jiu, Romania. FAU - Stoica, Maria AU - Stoica M AD - Department of Intensive Care and Anesthesia, Emergency County Hospital, 200349 Craiova, Romania. FAU - Teodora, Coanca Staicu Cristina AU - Teodora CSC AD - Research Methodology Department, Faculty of Pharmacy, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Stoica, George-Alin AU - Stoica GA AD - Department of Pediatric Surgery, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. LA - eng PT - Journal Article PT - Review DEP - 20260922 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708332 OTO - NOTNLM OT - AdipoR1 OT - AdipoR2 OT - PPARgamma OT - adiponectin OT - insulin resistance OT - natural products OT - receptor acetylation OT - receptor phosphorylation OT - selective PPARgamma modulator OT - translational pharmacology COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:44 PHST- 2026/09/07 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - ph19101507 [pii] AID - pharmaceuticals-19-01507 [pii] AID - 10.3390/ph19101507 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Sep 22;19(10):1507. doi: 10.3390/ph19101507. PMID- 42943202 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Sep 22 TI - Predicting Chemotherapy Response in Triple-Negative Breast Cancer: The Role of CYP2Cs as a Biomarker and a Drug Target (A Proof-of-Concept Study). LID - 10.3390/ph19101501 [doi] LID - 1501 AB - Background: Triple-negative breast cancer (TNBC) has a poor prognosis and limited targeted therapies, with anthracyclines being key treatments. However, many patients show reduced responsiveness to these treatments, affecting their outcomes. Arachidonic acid metabolites, specifically epoxyeicosatrienoic acids (EETs) produced by cytochrome P450 (CYP) epoxygenase enzymes, have been implicated in TNBC progression. However, limited data exist on the role of CYP epoxygenase gene expression in the responsiveness of anthracycline-based therapy in TNBC. Thus, we aimed to test the contribution of these CYPs in anthracycline responsiveness among patients with TNBC and to explore whether inhibition of CYP epoxygenase can potentiate the anti-cancer activity of anthracyclines. Methods: Our study used the KM-plotter database to retrieve and extract patients' data with TNBC. The patients were assigned to two cohorts: responders or less-responders to anthracycline based on relapse-free survival. Additionally, the effects of the CYP epoxygenase inhibitor MSPPOH in combination with doxorubicin were evaluated in the TNBC cell line. Results: The present study shows that TNBC patients with lower responsiveness to anthracycline-based therapy exhibited higher levels of CYP2C8, CYP2C9, and CYP2C19. We also found that these CYP2Cs could be potential biomarkers for predicting anthracycline responsiveness. Using human TNBC cells, we show that a selective CYP2C epoxygenase inhibitor, MSPPOH, significantly potentiates the anti-cancer effect of anthracycline. Conclusions: Collectively, this study provides preliminary evidence that CYP2C epoxygenase is involved in the therapeutic response to anthracyclines in TNBC, suggesting that these enzymes may serve as predictive biomarkers. However, given the limitations of using a public database and a single TNBC cell line, continued validation within larger, independent patient cohorts is needed. FAU - Zerouali, Nassima AU - Zerouali N AD - Department of Pharmaceutical Sciences, College of Pharmacy, QU Health, Qatar University, Doha 2713, Qatar. FAU - Ahmed, Ayesha AU - Ahmed A AD - Department of Pharmaceutical Sciences, College of Pharmacy, QU Health, Qatar University, Doha 2713, Qatar. FAU - Jawad, Sondus AU - Jawad S AD - Department of Pharmaceutical Sciences, College of Pharmacy, QU Health, Qatar University, Doha 2713, Qatar. FAU - Ghanem, Lana AU - Ghanem L AUID- ORCID: 0009-0004-2294-7243 AD - Department of Pharmaceutical Sciences, College of Pharmacy, QU Health, Qatar University, Doha 2713, Qatar. FAU - Dhulkifle, Hevna AU - Dhulkifle H AUID- ORCID: 0000-0002-1234-2273 AD - Department of Pharmaceutical Sciences, College of Pharmacy, QU Health, Qatar University, Doha 2713, Qatar. FAU - Abulola, Sara M AU - Abulola SM AD - Department of Pharmaceutical Sciences, College of Pharmacy, QU Health, Qatar University, Doha 2713, Qatar. FAU - Al-Maraghi, Ghalya AU - Al-Maraghi G AD - Department of Pharmaceutical Sciences, College of Pharmacy, QU Health, Qatar University, Doha 2713, Qatar. FAU - Al-Jumaily, Tamader AU - Al-Jumaily T AD - Department of Pharmaceutical Sciences, College of Pharmacy, QU Health, Qatar University, Doha 2713, Qatar. FAU - Therachiyil, Lubna AU - Therachiyil L AD - Translational Research Institute, Hamad Medical Corporation, Doha 3050, Qatar. FAU - Abunada, Hanan H AU - Abunada HH AUID- ORCID: 0000-0003-2186-2518 AD - Biomedical Research Center, QU Health, Qatar University, Doha 2713, Qatar. FAU - Al-Shar'i, Nizar A AU - Al-Shar'i NA AUID- ORCID: 0000-0002-1599-5704 AD - Department of Pharmaceutical Sciences, College of Pharmacy, QU Health, Qatar University, Doha 2713, Qatar. FAU - Maayah, Zaid H AU - Maayah ZH AD - Department of Pharmaceutical Sciences, College of Pharmacy, QU Health, Qatar University, Doha 2713, Qatar. LA - eng GR - QUCG-CPH-23/24-209/Qatar University/ PT - Journal Article DEP - 20260922 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708066 OTO - NOTNLM OT - CYP2C OT - anthracycline OT - epoxygenase OT - triple-negative breast cancer COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:44 PHST- 2026/03/31 00:00 [received] PHST- 2026/06/22 00:00 [revised] PHST- 2026/06/25 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - ph19101501 [pii] AID - pharmaceuticals-19-01501 [pii] AID - 10.3390/ph19101501 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Sep 22;19(10):1501. doi: 10.3390/ph19101501. PMID- 42943199 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Sep 22 TI - Synthesis of Antiproliferative N-Chalconyl Imidazolidones and Mechanistic Evaluation of Prototype N-CIMZ 1 as a Macrophage-Modulating Antimitotic Agent. LID - 10.3390/ph19101503 [doi] LID - 1503 AB - Background/Objectives: To address current aggressive breast cancer challenges, a novel series of eight N-chalconyl imidazolidone derivatives (N-CIMZs 1-8) was synthesized and evaluated for direct cytotoxicity and immunomodulation via macrophage polarization. Methods: Following antiproliferative screening against four representative breast cancer lines, the prototype candidate N-CIMZ 1 was selected for cell cycle and scratch-wound healing assays in MCF-7 cells. Multi-parametric flow cytometry on human THP-1 macrophages tracked cellular stress (gammaH2AX), metabolic markers (iNOS), and surface dynamics (LAP (TGF-beta1), CD163, CD206, CD209). Results:N-CIMZ 1 acted as an antimitotic agent, inducing G2/M phase arrest (51.0%) and restricting MCF-7 wound confluence to 33% at 24 h. In THP-1 macrophages, it maintained low induction of gammaH2AX-mediated DNA damage signaling. Under alternative M2 pressure, N-CIMZ 1 intercepted polarization by forcing high membrane retention of the immunosuppressive peptide LAP (81%) and suppressing the scavenger marker CD163 (12%) while buffering hyper-reactive CD206+/CD209+ hybrid spikes down to 2.33% under M1 stress. Conclusions:N-CIMZ 1 represents a promising multi-modal scaffold combining antimitotic and macrophage-modulating properties. This targeted immunomodulation effectively subverts tumor-supportive macrophage phenotypes, showcasing the potential of microenvironment reprogramming to counter breast cancer progression and metastasis. FAU - Chavez Alvarez, Atziri Corin AU - Chavez Alvarez AC AUID- ORCID: 0000-0002-5514-7144 AD - UMR-1240 IMoST INSERM, Universite Clermont Auvergne, 58 rue Montalembert, 63005 Clermont-Ferrand, France. FAU - Treboux, Lisa AU - Treboux L AUID- ORCID: 0009-0001-1696-5347 AD - UMR-1240 IMoST INSERM, Universite Clermont Auvergne, 58 rue Montalembert, 63005 Clermont-Ferrand, France. FAU - Bechon-Diot, Celine Audrey AU - Bechon-Diot CA AD - UMR-1240 IMoST INSERM, Universite Clermont Auvergne, 58 rue Montalembert, 63005 Clermont-Ferrand, France. FAU - Chkair, Rayan AU - Chkair R AUID- ORCID: 0009-0000-1530-6676 AD - UMR-1240 IMoST INSERM, Universite Clermont Auvergne, 58 rue Montalembert, 63005 Clermont-Ferrand, France. FAU - Besse, Sophie AU - Besse S AD - UMR-1240 IMoST INSERM, Universite Clermont Auvergne, 58 rue Montalembert, 63005 Clermont-Ferrand, France. FAU - Moreau, Emmanuel AU - Moreau E AUID- ORCID: 0000-0002-8004-5019 AD - UMR-1240 IMoST INSERM, Universite Clermont Auvergne, 58 rue Montalembert, 63005 Clermont-Ferrand, France. LA - eng GR - FEDER EPICURE 2019/FEDER EPICURE 2019, Microorganisms and biological tissues: from steady state to pa-thology, Incucyte system/ GR - (DRED_PIP_2023-008_IMOST_SPECT)/La Ligue contre le cancer/ GR - FRQS - studentship / bourse : 342994/FRQS/ PT - Journal Article DEP - 20260922 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708499 OTO - NOTNLM OT - N-chalconylimidazolidones OT - antimitotic agents OT - breast cancer OT - chalcones OT - imidazolidones OT - immunomodulation OT - macrophages COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:44 PHST- 2026/08/12 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - ph19101503 [pii] AID - pharmaceuticals-19-01503 [pii] AID - 10.3390/ph19101503 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Sep 22;19(10):1503. doi: 10.3390/ph19101503. PMID- 42943198 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1424-8247 (Print) IS - 1424-8247 (Electronic) IS - 1424-8247 (Linking) VI - 19 IP - 10 DP - 2026 Sep 22 TI - Beyond Weight Loss and Glycemic Control: Molecular Mechanisms and Hypothesis-Generating Therapeutic Potential of Incretin-Based Therapies Against Peritoneal Fibrosis in Peritoneal Dialysis. LID - 10.3390/ph19101502 [doi] LID - 1502 AB - Background: Peritoneal dialysis (PD) is an established kidney replacement therapy, but long-term exposure to bioincompatible dialysate, uremic toxins, recurrent peritonitis, glucose degradation products, oxidative stress, and sterile inflammation progressively remodels the peritoneal membrane and contributes to peritoneal fibrosis (PF). This review summarizes the molecular basis of PF in PD and evaluates the therapeutic rationale for incretin-based interventions beyond weight loss and glycemic control. Methods: This narrative review synthesizes mechanistic, translational, and clinically relevant literature on PD-associated PF, with emphasis on DPP-4 biology, GLP-1 receptor agonism, inflammatory and oxidative signaling, angiogenesis, mesothelial-to-mesenchymal transition, glycemic management in PD, and selected author-conducted translational studies interpreted alongside independent evidence. Results: Available evidence supports a model in which DPP-4 activation, altered GLP-1/GLP-1R signaling, TGF-beta/Smad3-driven mesothelial transition, NF-kappaB/MyD88 inflammation, ROS-mediated oxidative stress, angiogenesis, apoptosis, and cytoskeletal remodeling converge to impair peritoneal membrane integrity. DPP-4 inhibition, exendin-4, and dulaglutide attenuated these pathways in experimental models, while GLP-1 receptor agonists have established metabolic benefits in appropriate diabetic and obese populations. Conclusions: Direct clinical evidence that incretin-based therapies prevent or treat peritoneal fibrosis in humans undergoing PD is currently lacking. Therefore, peritoneal membrane protection, reduced ultrafiltration failure, and improved technique survival should be regarded as investigational, hypothesis-generating possibilities that require prospective clinical validation. FAU - Li, Yi-Chen AU - Li YC AD - Clinical Medicine Research Center, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701401, Taiwan. FAU - Hsu, Jui-Ting AU - Hsu JT AUID- ORCID: 0000-0002-1962-9429 AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833253, Taiwan. FAU - Fu, Chung-Ming AU - Fu CM AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833253, Taiwan. FAU - Liu, Ting-Chun AU - Liu TC AUID- ORCID: 0000-0002-2484-7084 AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833253, Taiwan. FAU - Lee, Yueh-Ting AU - Lee YT AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833253, Taiwan. AD - Division of Nephrology, Kaohsiung Municipal Fong-Shan Hospital, Kaohsiung 83062, Taiwan. FAU - Wu, Po-Jung AU - Wu PJ AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833253, Taiwan. AD - Division of Nephrology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung 80145, Taiwan. FAU - Li, Lung-Chih AU - Li LC AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833253, Taiwan. FAU - Chiou, Terry Ting-Yu AU - Chiou TT AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833253, Taiwan. FAU - Chiu, Chien-Hua AU - Chiu CH AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833253, Taiwan. FAU - Kuo, Wei-Hung AU - Kuo WH AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833253, Taiwan. FAU - Huang, Chiang-Chi AU - Huang CC AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833253, Taiwan. FAU - Tsai, Kai-Fan AU - Tsai KF AUID- ORCID: 0000-0002-0148-3302 AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833253, Taiwan. FAU - Hsu, Tsuen-Wei AU - Hsu TW AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833253, Taiwan. FAU - Lee, Wen-Chin AU - Lee WC AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833253, Taiwan. FAU - Lee, Chien-Te AU - Lee CT AUID- ORCID: 0000-0002-4704-1956 AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833253, Taiwan. AD - Division of Nephrology, Kaohsiung Municipal Fong-Shan Hospital, Kaohsiung 83062, Taiwan. FAU - Yang, Chih-Chao AU - Yang CC AD - Division of Nephrology, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung 833253, Taiwan. AD - Division of Nephrology, Kaohsiung Municipal Fong-Shan Hospital, Kaohsiung 83062, Taiwan. AD - Division of Nephrology, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung 80145, Taiwan. LA - eng PT - Journal Article PT - Review DEP - 20260922 PL - Switzerland TA - Pharmaceuticals (Basel) JT - Pharmaceuticals (Basel, Switzerland) JID - 101238453 PMC - PMC13708412 OTO - NOTNLM OT - DPP-4 OT - GLP-1 receptor agonist OT - TGF-beta OT - dulaglutide OT - incretin-based therapy OT - inflammation OT - mesothelial-to-mesenchymal transition OT - oxidative stress OT - peritoneal dialysis OT - peritoneal fibrosis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:44 PHST- 2026/07/24 00:00 [received] PHST- 2026/09/01 00:00 [revised] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - ph19101502 [pii] AID - pharmaceuticals-19-01502 [pii] AID - 10.3390/ph19101502 [doi] PST - epublish SO - Pharmaceuticals (Basel). 2026 Sep 22;19(10):1502. doi: 10.3390/ph19101502. PMID- 42943195 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Yield and Yield Components in Soybean Using Genome-Wide Association Studies, GWAS: Current Status, Challenges, and Breeding Prospects. LID - 10.3390/plants15203243 [doi] LID - 3243 AB - Molecular-genetic analysis of soybean yield and yield components under drought is a crucial element for the development of effective breeding strategies and sustainable agricultural production. The aim of this review is to critically analyse current data from genome-wide association studies (GWAS) and identified genetic loci, SNP markers, and candidate genes associated with yield components in soybean in dry conditions with limited water supply and adequate watering (controls). In the results, 346 genetic associations, and 229 candidate genes with KEGG-based potential functions were sufficiently described in published papers and selected for the current study. Three statistically significant metabolic pathways were identified with isoflavonoid biosynthesis, glycosaminoglycan degradation and cysteine and methionine metabolism. The main limitation of the presented analysis was found in the low level of heritability for the studied traits, together with human factors such as carrying out and interpreting GWAS affecting the results presented in the current study. Additionally, complex multigenic controls were reported for the studied traits in soybean and a high level of genotype x environment interactions. Nevertheless, the presented data provide a basis for developing molecular strategies to improve soybean yield in conjunction with better potential drought tolerance. FAU - Amangeldiyeva, Aigul AU - Amangeldiyeva A AD - Plant Biotechnology Laboratory, Kazakh Research Institute of Agriculture and Plant Growing, Almalybak 040909, Kazakhstan. AD - Department of Biology and Biotechnology, Farabi University, Almaty 050040, Kazakhstan. FAU - Didorenko, Svetlana AU - Didorenko S AD - Plant Biotechnology Laboratory, Kazakh Research Institute of Agriculture and Plant Growing, Almalybak 040909, Kazakhstan. FAU - Maikotov, Bekzhan AU - Maikotov B AD - Plant Biotechnology Laboratory, Kazakh Research Institute of Agriculture and Plant Growing, Almalybak 040909, Kazakhstan. FAU - Jenisbayeva, Assel AU - Jenisbayeva A AUID- ORCID: 0000-0002-1649-9681 AD - Plant Biotechnology Laboratory, Kazakh Research Institute of Agriculture and Plant Growing, Almalybak 040909, Kazakhstan. FAU - Shavrukov, Yuri AU - Shavrukov Y AUID- ORCID: 0000-0002-2941-0340 AD - College of Science and Engineering, Biological Sciences, Flinders University, Adelaide, SA 5042, Australia. FAU - Yerzhebayeva, Raushan AU - Yerzhebayeva R AUID- ORCID: 0000-0003-4585-8505 AD - Plant Biotechnology Laboratory, Kazakh Research Institute of Agriculture and Plant Growing, Almalybak 040909, Kazakhstan. LA - eng GR - BR22885857/Ministry of Agriculture of the Republic of Kazakhstan/ PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708931 OTO - NOTNLM OT - biological pathway OT - candidate gene OT - drought tolerance OT - genome-wide association study (GWAS) OT - marker-assisted selection (MAS) OT - soybean OT - water deficit OT - yield OT - yield components COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:44 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - plants15203243 [pii] AID - plants-15-03243 [pii] AID - 10.3390/plants15203243 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 21;15(20):3243. doi: 10.3390/plants15203243. PMID- 42943192 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Latitudinal Variation in the Phenology of the Emergent Species Phragmites australis and the Submerged Species Potamogeton crispus in Shallow Lakes of the North China Plain. LID - 10.3390/plants15203240 [doi] LID - 3240 AB - Phenology, as a pivotal functional trait, serves as an important indicator for understanding vegetation responses to environmental variability. Against the backdrop of climate change, the latitudinal zonation of vegetation phenology has attracted widespread attention. However, how the phenology of different aquatic plants responds to latitudinal variation remains insufficiently understood. To characterize the contrasting responses of aquatic vegetation (submerged and emergent) along a latitudinal gradient, this study focuses on the submerged species Potamogeton crispus (P. crispus) and the emergent species Phragmites australis (P. australis), both of which are widely distributed across shallow lakes on the North China Plain. Based on dense time-series Normalized Difference Vegetation Index (NDVI) data from Sentinel-2 and a standardized phenological extraction method, we analyzed the spatial differentiation patterns of these two vegetation types, as well as their latitudinal phenological responses and environmental associations, including the Start of the growing Season (SOS), End of the growing Season (EOS), and length of the growing season (LOS). The results showed marked differences in the spatial distributions of P. crispus and P. australis, reflecting their distinct ecological niches within lake ecosystems. Their phenological metrics also exhibited contrasting latitudinal patterns. For P. australis, SOS showed no significant latitudinal trend, whereas EOS occurred progressively earlier and LOS became shorter with increasing latitude. For P. crispus, both SOS and EOS were delayed with increasing latitude, while LOS showed no significant latitudinal gradient. Among the linear predictors, annual mean temperature showed the highest overall importance, but its associations with the phenology of P. australis and P. crispus differed markedly. Other environmental and anthropogenic factors also showed species- and stage-specific associations with phenological variation. XGBoost-SHAP analysis (Extreme Gradient Boosting (XGBoost) combined with SHapley Additive exPlanations (SHAP)) further revealed nonlinear marginal associations between selected environmental predictors and phenological metrics, including an apparent change in the modeled EOS response of P. australis near 16 degrees C, which should be interpreted as an exploratory model-derived feature rather than a confirmed threshold. This study highlights contrasting latitudinal phenological responses of submerged and emergent macrophytes and provides scientific support for ecological monitoring and management of shallow lake ecosystems in the North China Plain. FAU - Tang, Jia AU - Tang J AD - School of Geography and Environment, Liaocheng University, Liaocheng 252059, China. FAU - Yu, Quanzhou AU - Yu Q AUID- ORCID: 0000-0002-6123-0147 AD - School of Geography and Environment, Liaocheng University, Liaocheng 252059, China. AD - Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China. FAU - Li, Yong AU - Li Y AD - School of Land Resources and Surveying and Mapping Engineering, Shandong Agriculture and Engineering University, Jinan 250100, China. FAU - Zhang, Hao AU - Zhang H AD - Shandong Huaao Geographic Information Co., Ltd., Jinan 250000, China. FAU - Ma, Jun AU - Ma J AD - School of Geography and Environment, Liaocheng University, Liaocheng 252059, China. FAU - Xiao, Shuwen AU - Xiao S AD - School of Geography and Environment, Liaocheng University, Liaocheng 252059, China. FAU - Liang, Tianquan AU - Liang T AUID- ORCID: 0000-0003-3426-412X AD - School of Geography and Environment, Liaocheng University, Liaocheng 252059, China. FAU - Tang, Qingxin AU - Tang Q AUID- ORCID: 0000-0002-5451-0546 AD - School of Geography and Environment, Liaocheng University, Liaocheng 252059, China. FAU - Wang, Shaoqiang AU - Wang S AUID- ORCID: 0000-0002-4111-6689 AD - Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China. LA - eng GR - 31800367/National Natural Science Foundation of China/ GR - ZR2023MD129/Natural Science Foundation of Shandong Province/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13709194 OTO - NOTNLM OT - North China Plain OT - aquatic plant phenology OT - environmental associations OT - latitudinal zonality OT - shallow lakes COIS- Author Hao Zhang was employed by the company Shandong Huaao Geographic Information Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:44 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - plants15203240 [pii] AID - plants-15-03240 [pii] AID - 10.3390/plants15203240 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 21;15(20):3240. doi: 10.3390/plants15203240. PMID- 42943188 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Harnessing the Phytochemical Diversity of the Genus Astragalus for Evolving Cosmeceutical Applications. LID - 10.3390/plants15203236 [doi] LID - 3236 AB - The genus Astragalus L. (Fabaceae) comprises approximately 3000 species with a rich history of use in traditional medicine worldwide. While Astragalus membranaceus Fisch. ex Bunge is globally recognized as an ingredient in dietary supplements, the cosmetic potential of the broader genus remains largely underexplored. Hence, the current review aims to systematically evaluate 20 Astragalus species selected for their documented or prospective applications in the cosmeceutical industry. We examine the diverse phytochemical profiles of these species, highlighting the principal groups of bioactive constituents such as flavonoids (quercetin, kaempferol), saponins (astragaloside IV), and polysaccharides (arabino-3,6-galactan). These compounds have been reported to be associated with critical dermatological benefits, including potent antioxidant activity, skin-lightening effects, anti-inflammatory activity relevant to conditions such as atopic dermatitis, and stimulation of collagen and hyaluronic acid synthesis for anti-aging benefits. By bridging the gap between traditional ethnobotanical use of the selected Astragalus species and modern evidence-based scientific formulations, this review provides a roadmap for integrating diverse Astragalus species into sustainable, high-performance cosmetic products. Unlike the existing literature, which focuses primarily on A. membranaceus, this review provides, to our knowledge, the first systematic comparative analysis of 20 diverse Astragalus species, mapping their unique phytochemical markers to specific molecular pathways for advanced cosmeceutical applications. FAU - Shahrivari-Baviloliaei, Saba AU - Shahrivari-Baviloliaei S AUID- ORCID: 0000-0002-2721-5592 AD - Department of Analytical Chemistry, Faculty of Pharmacy, Medical University of Gdansk, 80-210 Gdansk, Poland. AD - Chemical Analysis Laboratory Lab-Spark Sp. o.o., 81-451 Gdynia, Poland. FAU - Orhan, Ilkay Erdogan AU - Orhan IE AUID- ORCID: 0000-0002-7379-5436 AD - Department of Pharmacognosy, Faculty of Pharmacy, Lokman Hekim University, 06510 Ankara, Turkiye. FAU - Viapiana, Agnieszka AU - Viapiana A AUID- ORCID: 0000-0002-4398-1780 AD - Department of Analytical Chemistry, Faculty of Pharmacy, Medical University of Gdansk, 80-210 Gdansk, Poland. FAU - Plenis, Alina AU - Plenis A AUID- ORCID: 0000-0002-0507-2770 AD - Department of Analytical Chemistry, Faculty of Pharmacy, Medical University of Gdansk, 80-210 Gdansk, Poland. LA - eng GR - BPI/STE/2023/1/00013/U/00001/Narodowa Agencja Wymiany Akademickiej/ PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13709146 OTO - NOTNLM OT - Astragalus OT - anti-inflammatory OT - antioxidant OT - compounds OT - cosmetics OT - skin COIS- Saba Shahrivari-Baviloliaei was employed by the company Lab-Spark Sp o.o. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:44 PHST- 2026/08/10 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - plants15203236 [pii] AID - plants-15-03236 [pii] AID - 10.3390/plants15203236 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 21;15(20):3236. doi: 10.3390/plants15203236. PMID- 42943183 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Revisiting the Endophytic Fungi Residing Within Axonopus compressus: Molecular Identification, Antagonistic Potential, and Extracellular Enzyme Activities. LID - 10.3390/plants15203229 [doi] LID - 3229 AB - Endophytic fungi colonize plant tissues asymptomatically and may contribute to host fitness and protection against biotic stresses. This study characterized the endophytic fungal community associated with healthy leaves of lawn grass (Axonopus compressus) and evaluated the extracellular enzyme activities and antagonistic potential of the recovered isolates against selected banana crown rot pathogens. Molecular identification revealed a diverse assemblage of fungal taxa, including species of Curvularia, Colletotrichum, Diaporthe, Xylaria, Fusarium, Penicillium, and Aspergillus. In dual-culture assay, all tested isolates exhibited antagonistic activity against the target pathogens, with mycelial growth inhibition ranging from 16.67% to 70.00%. The isolates displayed variable extracellular enzyme profiles, producing cellulase, protease, lipase, pectinase, and amylase at different levels. These differences in enzyme activities suggest functional differentiation among the endophytic fungi and may reflect distinct resource-use strategies within the leaf microenvironment. The observed antagonistic activity may be associated, at least in part, with extracellular enzyme production, although the contribution of individual enzymes to pathogen suppression requires further investigation. Overall, the findings highlight A. compressus as a reservoir of functionally diverse, enzyme-producing fungal endophytes with antagonistic potential against banana crown rot pathogens, providing promising candidates for further evaluation as biological control agents for sustainable banana disease management. FAU - Kausar, Rizwana AU - Kausar R AUID- ORCID: 0000-0003-2583-7272 AD - School of Biological Sciences, Universiti Sains Malaysia, Minden, Pulau Pinang 11800 USM, Malaysia. FAU - Azuddin, Nurul Farizah AU - Azuddin NF AUID- ORCID: 0000-0002-3638-3993 AD - School of Biological Sciences, Universiti Sains Malaysia, Minden, Pulau Pinang 11800 USM, Malaysia. FAU - Zakaria, Latiffah AU - Zakaria L AUID- ORCID: 0000-0002-2388-1424 AD - School of Biological Sciences, Universiti Sains Malaysia, Minden, Pulau Pinang 11800 USM, Malaysia. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708824 OTO - NOTNLM OT - Axonopus compressus OT - antifungal activity OT - biological control OT - fungal endophytes OT - hydrolytic enzymes OT - molecular identification COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:44 PHST- 2026/09/21 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - plants15203229 [pii] AID - plants-15-03229 [pii] AID - 10.3390/plants15203229 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 21;15(20):3229. doi: 10.3390/plants15203229. PMID- 42943171 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Mangrove-Derived Halotolerant Endophytic Bacteria Confer Strain-Specific and Consortium-Mediated Salt Tolerance in Peanut (Arachis hypogaea L.). LID - 10.3390/plants15203218 [doi] LID - 3218 AB - Soil salinity is a major abiotic stress that severely limits crop productivity worldwide. This study evaluated the potential of halotolerant endophytes isolated from mangrove plants to enhance salinity tolerance in the peanut (Arachis hypogaea L. cv. JL24). A two-step approach, including screening and validation methods, was applied. Initially, six mangrove endophytes, ACR52, EAR173, EAR191, EAR195, EAL318, and ACL463, were tested for their capacity to colonize the non-host peanut. Histochemical detection of gusA-tagged derivatives confirmed early tissue-associated colonization of peanut seedlings by all six isolates. Seed inoculation of six endophytes improved germination under severe salinity, with ACR52 maintaining 83% final germination at 200 mM NaCl, compared with 67% in the uninoculated controls. The six-strain consortium accelerated germination, giving the highest germination at every NaCl level on Days 1 and 4. Among the isolates, EAL318 (Bacillus subtilis), EAR195 (Proteus mirabilis), and ACR52 (Klebsiella quasipneumoniae subsp. similipneumoniae) exhibited strong PGP traits and were selected for further evaluation. In greenhouse experiments with 300 and 500 mM NaCl, inoculated plants showed significantly higher plant height (32-40% increase), shoot dry weight (28-35% increase), and chlorophyll content (20-30% increase) compared with uninoculated controls. Under severe greenhouse salinity, ACR52 showed the most consistent preservation of reproductive performance, whereas C3 produced the broadest antioxidant response, with elevated CAT and POD activities and reduced H(2)O(2) and MDA accumulation. These findings identify mangrove-derived endophytes as potential candidates for future field evaluation under saline-soil conditions. FAU - Yammanuri, Nagarathnamma AU - Yammanuri N AD - Department of Botany, School of Life Sciences, Yogi Vemana University, Kadapa 516005, India. AD - Department of Botany, SKR & SKR Government College for Women (A), Kadapa 516001, India. FAU - Chinreddy, Subramanyam Reddy AU - Chinreddy SR AUID- ORCID: 0000-0001-9641-2314 AD - Department of Biology, Gus R. Douglass Institute, West Virginia State University, Institute, WV 25112, USA. FAU - Naik Kt, Preetham AU - Naik Kt P AD - Department of Botany, School of Life Sciences, Yogi Vemana University, Kadapa 516005, India. FAU - Vallepu, Vishnu Babu AU - Vallepu VB AD - Department of Botany, School of Life Sciences, Yogi Vemana University, Kadapa 516005, India. FAU - Akila, Chandra Sekhar AU - Akila CS AUID- ORCID: 0000-0001-8799-835X AD - Department of Biotechnology, School of Life Sciences, Yogi Vemana University, Kadapa 516005, India. FAU - Puli, Chandra Obul Reddy AU - Puli COR AUID- ORCID: 0000-0002-0660-3852 AD - Department of Botany, School of Life Sciences, Yogi Vemana University, Kadapa 516005, India. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708864 OTO - NOTNLM OT - GUS-mediated colonization OT - antioxidant defense OT - bacterial consortium OT - mangrove endophytes OT - peanut OT - salinity COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:44 PHST- 2026/07/03 00:00 [received] PHST- 2026/08/09 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - plants15203218 [pii] AID - plants-15-03218 [pii] AID - 10.3390/plants15203218 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 20;15(20):3218. doi: 10.3390/plants15203218. PMID- 42943169 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Fire-Disturbed Soil and Intraspecific Competition Modulate Juvenile Performance of Invasive Hakea decurrens subsp. physocarpa. LID - 10.3390/plants15203217 [doi] LID - 3217 AB - Fire has historically been an important component of Mediterranean ecosystems, where many invasive alien plant species (IAPS) spread by taking advantage of fire disturbance, out-competing native species and creating landscapes with novel fire regimes. In the serotinous woody IAPS Hakea decurrens subsp. physocarpa (hereafter H. decurrens for simplicity), fire promotes fruit opening and subsequent recruitment, but the contribution of fire-disturbed soil and conspecific density to its establishment remains poorly understood. Thus, the present study evaluated the responses of juvenile H. decurrens to local natural soil (NS) and fire-disturbed soil (DS), in the absence (NC) or presence (WC) of intraspecific competition, during a 12-month outdoor experiment. A preliminary assay also characterized seedling emergence under different light conditions (full, moderate and low) to mimic the gap effect caused by fires. Seedling emergence ranged from 78.9% to 97.4%, with the lowest values observed under low light conditions. Soil condition did not affect relative height growth rate, whereas intraspecific competition significantly reduced growth rate and most final growth traits. Compared with NS, DS selectively altered shoot biomass, root development, proteoid root formation, leaf pigments, carbon metabolism and mineral composition, and was associated with higher easily extractable glomalin-related soil protein values, but did not consistently enhance overall plant growth. Soil x competition interactions were particularly evident for biomass allocation and carbohydrate metabolism. Principal component analysis revealed distinct functional profiles among treatment combinations. Overall, DS did not consistently enhance juvenile growth of H. decurrens. On the contrary, plants maintained performance through coordinated belowground, physiological, biochemical and nutritional adjustments, whereas intraspecific competition emerged as the strongest constraint on individual performance. FAU - Morais, Maria Cristina AU - Morais MC AUID- ORCID: 0000-0002-6598-7454 AD - Centre for the Research and Technology of Agroenvironmental and Biological Sciences, CITAB, Inov4Agro, Universidade de Tras-os-Montes e Alto Douro, UTAD, Quinta de Prados, 5000-801 Vila Real, Portugal. FAU - Ferreira, Helena AU - Ferreira H AD - Centre for the Research and Technology of Agroenvironmental and Biological Sciences, CITAB, Inov4Agro, Universidade de Tras-os-Montes e Alto Douro, UTAD, Quinta de Prados, 5000-801 Vila Real, Portugal. FAU - Sousa, Joao Ricardo AU - Sousa JR AUID- ORCID: 0000-0003-4365-9086 AD - Centre for the Research and Technology of Agroenvironmental and Biological Sciences, CITAB, Inov4Agro, Universidade de Tras-os-Montes e Alto Douro, UTAD, Quinta de Prados, 5000-801 Vila Real, Portugal. FAU - Cabral, Joao Alexandre AU - Cabral JA AUID- ORCID: 0000-0002-3333-8898 AD - Centre for the Research and Technology of Agroenvironmental and Biological Sciences, CITAB, Inov4Agro, Universidade de Tras-os-Montes e Alto Douro, UTAD, Quinta de Prados, 5000-801 Vila Real, Portugal. FAU - Goncalves, Berta AU - Goncalves B AUID- ORCID: 0000-0002-5764-024X AD - Centre for the Research and Technology of Agroenvironmental and Biological Sciences, CITAB, Inov4Agro, Universidade de Tras-os-Montes e Alto Douro, UTAD, Quinta de Prados, 5000-801 Vila Real, Portugal. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13709271 OTO - NOTNLM OT - Mediterranean ecosystems OT - functional traits OT - invasive alien plant species OT - post-fire response OT - seedling emergence COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:44 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - plants15203217 [pii] AID - plants-15-03217 [pii] AID - 10.3390/plants15203217 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 20;15(20):3217. doi: 10.3390/plants15203217. PMID- 42943165 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 19 TI - Reproductive Biology, Pollination Optimization, and Hybrid Verification of Acacia mangium x A. auriculiformis. LID - 10.3390/plants15203212 [doi] LID - 3212 AB - Acacia mangium x A. auriculiformis (hybrid acacia) combines high productivity with strong ecological adaptability and is therefore an important species for plantation establishment and germplasm innovation in southern China. Yet large-scale seed production has been constrained in Fujian by asynchronous flowering of the two parents, interspecific reproductive barriers, and the low efficiency of artificial pollination. The results show obvious spatial and temporal differences in the selected A. mangium, providing a basis for identifying suitable pollination windows. Ultra-low-temperature storage effectively maintained the long-term viability of A. auriculiformis pollen, providing a practical approach to alleviate asynchronous flowering between the parental species. Optimization of the liquid-pollination nutrient solution, pollen suspension system, and mentor-pollen proportion resulted in relatively high pod-set rates under field conditions for selected treatments. SLAF-seq generated 230,580 SLAF tags and 221,240 population-level SNPs. Combined evidence from SNP genotype concordance, principal component analysis, population structure, and phylogenetic analysis indicates that ZD15 and ZD16 show the strongest genetic evidence consistent with hybrid origin and are therefore regarded as the putative hybrid progeny with the strongest current genetic support, while M2 x DY5 is the most strongly supported candidate parental combination. These findings provide theoretical and technical support for germplasm innovation in hybrid acacia and for the development of more efficient and precise hybrid breeding strategies in Acacia. FAU - Yang, Yating AU - Yang Y AD - College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Ding, Zhenning AU - Ding Z AD - College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Huang, Simin AU - Huang S AD - College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Fan, Yanxin AU - Fan Y AD - College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Lu, Sheng AU - Lu S AD - College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Su, Bigui AU - Su B AD - College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Han, Tianran AU - Han T AD - College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Mu, Shiyan AU - Mu S AD - College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Chen, Yu AU - Chen Y AUID- ORCID: 0009-0001-9208-4103 AD - Key Laboratory for Forest Stress Physiological Ecology and Molecular Biology of Fujian Provincial, Department of Education, College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Ding, Guochang AU - Ding G AUID- ORCID: 0000-0001-8663-3041 AD - College of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou 350002, China. LA - eng GR - ZMGG-0810/Fujian Provincial Department of Science and Technology/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13709013 OTO - NOTNLM OT - Acacia mangium x A. auriculiformis OT - liquid pollination OT - long-term pollen storage OT - paternity testing COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:44 PHST- 2026/09/05 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - plants15203212 [pii] AID - plants-15-03212 [pii] AID - 10.3390/plants15203212 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 19;15(20):3212. doi: 10.3390/plants15203212. PMID- 42943163 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 19 TI - Phenotypic and Molecular Diversity of Salicornia bigelovii and Its Potential for Salinity-Resilient Industrial Oilseed Production. LID - 10.3390/plants15203210 [doi] LID - 3210 AB - Salicornia bigelovii is a promising oilseed crop known for its high yield potential and exceptional salt tolerance, ranking among the most salt-resistant vascular plants. Although previous studies have investigated individual phenotypic and genetic characteristics of S. bigelovii, integrated assessments combining agronomic performance, seed morphometric variation, and genetic relationships among geographically distinct accessions remain limited. This study evaluated six S. bigelovii accessions from the Texas Gulf coastline, focusing on phenotypic and morphometric traits, geographic origins, and genomic distances. Significant variability was observed among accessions in key traits, such as plant height, spike number, root length, and seed yield. Notably, the South Padre and Boca Chica accessions demonstrated superior growth and higher seed productivity compared with Corpus Christi Bridge and Galveston accessions, indicating substantial phenotypic diversity and potential for selecting high-performing accessions for breeding. Morphological analysis revealed minor but distinct differences among the six seed accessions. The southern accessions, SPN and SP, exhibited advantageous traits, including a high surface area, circularity values close to 1, and increased seed volume, suggesting favorable dispersal and flow characteristics. Multivariate analyses and hierarchal clustering showed significant relationships among accessions, providing insights for breeding programs. Phylogenetic analysis indicated the divergence of CCB and G accessions from BC, BCW, SP, and SPN. This morphological and genetic assessment establishes a foundation for accelerated molecular breeding and genomic-assisted improvement of S. bigelovii as an industrial crop. FAU - AlYammahi, Jawaher AU - AlYammahi J AUID- ORCID: 0000-0002-3870-1465 AD - Department of Chemical and Petroleum Engineering, Khalifa University, Abu Dhabi P.O. Box 127788, United Arab Emirates. AD - Sustainable Bioenergy Research Consortium, Khalifa University, Abu Dhabi P.O. Box 127788, United Arab Emirates. AD - Division of Engineering, New York University Abu Dhabi, Abu Dhabi P.O. Box 129188, United Arab Emirates. FAU - Chelaifa, Houda AU - Chelaifa H AUID- ORCID: 0000-0002-1466-3441 AD - Department of Chemical and Petroleum Engineering, Khalifa University, Abu Dhabi P.O. Box 127788, United Arab Emirates. AD - Sustainable Bioenergy Research Consortium, Khalifa University, Abu Dhabi P.O. Box 127788, United Arab Emirates. AD - Crop Diversification and Genetics Program, International Center for Biosaline Agriculture (ICBA), Dubai P.O. Box 14660, United Arab Emirates. FAU - Mohammed, Bushra G AU - Mohammed BG AD - Department of Pharmaceutical Chemistry, Shadan Collage of Pharmacy, Hyderabad 500091, India. FAU - Hernandez, Hector H AU - Hernandez HH AUID- ORCID: 0000-0001-7173-1829 AD - Sustainable Bioenergy Research Consortium, Khalifa University, Abu Dhabi P.O. Box 127788, United Arab Emirates. LA - eng GR - EX2016-000023/Sustainable Bioenergy Research Consortium, Khalifa University/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708847 OTO - NOTNLM OT - Salicornia bigelovii OT - halophyte OT - morphology OT - phenotypes OT - phylogeny OT - seeds COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:44 PHST- 2026/08/30 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - plants15203210 [pii] AID - plants-15-03210 [pii] AID - 10.3390/plants15203210 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 19;15(20):3210. doi: 10.3390/plants15203210. PMID- 42943156 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 19 TI - Hydrochar and Arbuscular Mycorrhizal Fungi Improve Soil Properties and Plant Growth in Coal Gangue-Amended Sandy Soil. LID - 10.3390/plants15203201 [doi] LID - 3201 AB - This study evaluated hydrochar and arbuscular mycorrhizal fungi (AMF) for improving coal gangue-amended sandy soil and plant performance. A ryegrass (Lolium perenne L.) pot experiment compared corncob, corn straw, and rice husk hydrochars applied at 2-8%. Selected 8% hydrochars were then evaluated in a four-month alfalfa (Medicago sativa L.) rhizobox experiment to examine soil and plant responses across a 30 cm soil profile and the additional response to AMF inoculation. Hydrochar significantly affected soil nutrient status and all seven ryegrass growth and root traits, with 8% rice husk hydrochar (RHH8) showing the strongest overall performance. After cultivation, soil organic matter was 32.85 g kg(-1) under RHH8 versus 14.81 g kg(-1) under the control (CK). In the rhizobox experiment, compared with 8% rice husk hydrochar alone (RHH), AMF addition to RHH was associated with higher soil nutrient levels, greater dry-sieved aggregation and water retention, and a greater maximum root length in alfalfa (33.46 vs. 25.68 cm). Total soil concentrations of all eight measured metals were below the GB 15618-2018 agricultural-land risk screening values at the measured endpoints. Overall, RHH performed consistently well, with additional improvements following AMF inoculation. These short-term findings support further evaluation for restoring coal gangue-amended sandy soil. FAU - Song, Huiping AU - Song H AUID- ORCID: 0000-0001-8083-1960 AD - Institute of Resources and Environmental Engineering, Shanxi University, Taiyuan 030031, China. AD - Changzhi Key Laboratory for Testing and Application of Solid Waste New Materials, Changzhi 046000, China. FAU - Zhang, Rui AU - Zhang R AD - Institute of Resources and Environmental Engineering, Shanxi University, Taiyuan 030031, China. FAU - Zhang, Junjie AU - Zhang J AD - Institute of Resources and Environmental Engineering, Shanxi University, Taiyuan 030031, China. FAU - Xue, Zhanjin AU - Xue Z AD - Institute of Resources and Environmental Engineering, Shanxi University, Taiyuan 030031, China. AD - Changzhi Key Laboratory for Testing and Application of Solid Waste New Materials, Changzhi 046000, China. FAU - Wang, Xiaohui AU - Wang X AD - Anhui Provincial Academy of Eco-Environmental Science Research, Hefei 230061, China. FAU - Zhao, Yan AU - Zhao Y AD - Anhui Provincial Academy of Eco-Environmental Science Research, Hefei 230061, China. FAU - Feng, Zhengjun AU - Feng Z AUID- ORCID: 0000-0002-0010-3283 AD - Institute of Resources and Environmental Engineering, Shanxi University, Taiyuan 030031, China. LA - eng GR - 2024YFC3909300/National Key Research and Development Program of China/ GR - 202303021221083/General Program of Basic Research of Shanxi Province/ GR - 202523anull050017/Anhui Provincial Science and Technology Innovation Tackling Program/ GR - Qian Ke He Major [2025]019/Guizhou Provincial Science and Technology Plan Project/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708921 OTO - NOTNLM OT - arbuscular mycorrhizal fungi OT - coal gangue OT - hydrochar OT - plant growth OT - sandy soil OT - soil restoration COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:44 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - plants15203201 [pii] AID - plants-15-03201 [pii] AID - 10.3390/plants15203201 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 19;15(20):3201. doi: 10.3390/plants15203201. PMID- 42943154 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 19 TI - Host Identity and Urban Space Structure Phyllosphere Bacterial Communities Through Compositional Turnover in a Tropical Andean City. LID - 10.3390/plants15203202 [doi] LID - 3202 AB - Phyllosphere bacterial communities of urban vegetation remain poorly characterised in tropical Andean cities, where high year-round ultraviolet irradiance and compact valley topography differ from the temperate systems that dominate the literature. We characterised epiphytic bacterial communities on fifteen plants of three widely planted species: Mangifera indica L. (mango; Anacardiaceae), Terminalia catappa L. (tropical almond; Combretaceae) and Thunbergia grandiflora (Roxb. ex Rottler) Roxb. (blue trumpet vine; Acanthaceae), one per species in each of five urban zones of Medellin, Colombia, sampled in five surveys over nineteen months with three spatial subsamples per plant and survey (224 samples; 16S rRNA V3-V4). Host species and urban zone structured community composition across all five distance metrics examined (R(2) = 0.19-0.30 and 0.34-0.37), yet neither affected any measure of alpha diversity. Partitioning beta diversity resolved why: host species and urban zone acted exclusively through taxon turnover, whereas survey acted exclusively through nestedness, driving a fivefold decline in richness that tracked a monotonic rise in relative humidity across the study period. The two spatial filters explained statistically independent fractions of variation (unique adjusted R(2) = 0.236 and 0.174; shared fraction indistinguishable from zero). Bacterial communities from different parts of a single canopy differed by 69% of the dissimilarity separating host species, and this within-plant coherence weakened as communities contracted. Deterministic host selection and stochastic temporal contraction therefore appear to operate concurrently through complementary mechanisms in this system. FAU - Munera Porras, Luisa Maria AU - Munera Porras LM AUID- ORCID: 0000-0002-0215-9993 AD - Research Group on Health and Sustainability, School of Microbiology, University of Antioquia, Calle 70 No. 52-21, Medellin 050010, Colombia. FAU - Londono, Santiago Garcia AU - Londono SG AD - Diagnostic and Pollution Control Research Group (GDCON), School of Microbiology, University of Antioquia, Calle 70 No. 52-21, Medellin 050010, Colombia. FAU - Rodriguez Cabal, Hector Alejandro AU - Rodriguez Cabal HA AD - Grupo de Biotecnologia Vegetal, Facultad de Ciencias Agrarias, Universidad Nacional de Colombia Sede Medellin, Medellin 050034, Colombia. FAU - Perez, Miguel AU - Perez M AD - Biosciences Research Group, Faculty of Health Sciences, University Institution Colegio Mayor de Antioquia, Medellin 050036, Colombia. FAU - Pino, Nancy J AU - Pino NJ AD - Diagnostic and Pollution Control Research Group (GDCON), School of Microbiology, University of Antioquia, Calle 70 No. 52-21, Medellin 050010, Colombia. LA - eng GR - code 111591891754, contract 585-2022./Ministerio de Ciencia, Tecnologia e Innovacion/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708965 OTO - NOTNLM OT - 16S rRNA OT - community assembly OT - epiphytic bacteria OT - nestedness OT - phyllosphere OT - tropical Andes OT - turnover OT - urban ecology COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:44 PHST- 2026/08/01 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - plants15203202 [pii] AID - plants-15-03202 [pii] AID - 10.3390/plants15203202 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 19;15(20):3202. doi: 10.3390/plants15203202. PMID- 42943149 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 18 TI - Ameliorative Mechanisms of ZnO Nanoparticles in Salinity-Stressed Basil Callus: From Redox Defense to Metabolomic Normalization. LID - 10.3390/plants15203196 [doi] LID - 3196 AB - Salinity is a major abiotic stressor inducing osmotic imbalance, ion toxicity, and oxidative damage in plant cells. This study investigates the ameliorative effects of 100 mg L(-1) zinc oxide nanoparticles (ZnO NPs) on Ocimum basilicum L. callus cultures under salinity stress (0-90 mM NaCl) through an integrated enzymatic and GC-MS metabolomic approach. Exposure to 90 mM NaCl significantly suppressed biomass and increased oxidative stress markers (H(2)O(2) and MDA). Notably, ZnO NP supplementation exhibited a selective modulatory role, preferentially stimulating the ascorbate-glutathione cycle, with ascorbate peroxidase activity reaching a peak of 1.16 U mg(-1) protein. While ZnO NPs alone caused a slight increase in oxidative stress indicators, they significantly mitigated severe stress by reducing H(2)O(2) levels and stabilizing protein content. Metabolomic profiling revealed that salinity triggered a shift toward sesquiterpene hydrocarbons (79.71%), whereas ZnO NP application reprogrammed secondary metabolism toward ester compounds (40.50%) and oxygenated metabolites, effectively normalizing salt-perturbed metabolite pools. Principal component analysis explained 72.5% of total variation, clearly distinguishing nanoparticle-driven recovery from stress-driven damage. These findings demonstrate that ZnO NPs enhance stress tolerance by reinforcing metabolic bridges between antioxidant defense and biomass preservation, offering a robust strategy for nano-enabled resilience. FAU - Tabay Sumbul, Dilruba AU - Tabay Sumbul D AD - Central Research and Application Laboratories, Agri Ibrahim Cecen University, 04100 Agri, Turkiye. FAU - Turan Buyukdinc, Damla AU - Turan Buyukdinc D AUID- ORCID: 0000-0002-2776-1008 AD - Department of Horticulture, Faculty of Agriculture, Recep Tayyip Erdogan University, 53300 Rize, Turkiye. FAU - Ozcan Aykutlu, Aysel AU - Ozcan Aykutlu A AD - Department of Field Crops, Faculty of Agriculture, Recep Tayyip Erdogan University, 53300 Rize, Turkiye. LA - eng GR - Recep Tayyip Erdogan University/ PT - Journal Article DEP - 20261018 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13709176 OTO - NOTNLM OT - GC-MS metabolomics OT - Ocimum basilicum L. OT - antioxidant enzymes OT - callus culture OT - metabolic reprogramming OT - reactive oxygen species OT - salinity stress OT - zinc oxide nanoparticles COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:44 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - plants15203196 [pii] AID - plants-15-03196 [pii] AID - 10.3390/plants15203196 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 18;15(20):3196. doi: 10.3390/plants15203196. PMID- 42943142 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 18 TI - Silicon Modulates the Expression of Copper-Responsive Transporters and Non-Coding RNAs in Cucumber Subjected to Copper Stress. LID - 10.3390/plants15203189 [doi] LID - 3189 AB - Excess copper (Cu) disrupts metal homeostasis and induces oxidative stress in plants. Silicon (Si) is known to mitigate heavy metal toxicity, yet the molecular basis of its protective role under Cu stress remains insufficiently understood. To address this, an exploratory transcriptome-wide screening was performed to identify candidate differentially expressed genes (DEGs), long non-coding RNAs (lncRNAs), and microRNAs responsive to excess Cu and/or Si in cucumber (Cucumis sativus cv. Chinese Long). Genes encoding metal transporters and selected miRNA-target pairs were further analyzed by qRT-PCR. Silicon alone modulated the expression of numerous candidate DEGs, notably enhancing the expression of metal transporter genes. Exposure to excess Cu suppressed genes involved in metal uptake while upregulating those associated with efflux and metal redistribution. Application of Si under Cu stress mitigated these transcriptional alterations. Cu stress repressed miR398 and miR408, concomitantly increasing the expression of their targets: superoxide dismutase (SOD) and plantacyanin (PCY). Furthermore, excess Cu downregulated miR390, resulting in increased expression of its predicted lncRNA target. The addition of Si to Cu-stressed plants further enhanced SOD expression while attenuating the upregulation of PCY and the lncRNA. The results demonstrated that Si modulates both coding and non-coding RNA expression under optimal and Cu-stress conditions, revealing post-transcriptional gene expression regulation as an additional layer of Si's effect on plants. When integrated with previously reported physiological and biochemical data, these findings advance our understanding of the molecular basis of Si-mediated heavy metal tolerance in plants. FAU - Bosnic, Dragana AU - Bosnic D AUID- ORCID: 0000-0001-5182-4438 AD - Institute of Molecular Genetics and Genetic Engineering, Department of Microbiology and Plant Biology, University of Belgrade, Vojvode Stepe 444a, 11042 Belgrade, Serbia. FAU - Timotijevic, Gordana AU - Timotijevic G AD - Institute of Molecular Genetics and Genetic Engineering, Department of Microbiology and Plant Biology, University of Belgrade, Vojvode Stepe 444a, 11042 Belgrade, Serbia. FAU - Nikolic, Dragana AU - Nikolic D AUID- ORCID: 0000-0002-9812-062X AD - Institute of Molecular Genetics and Genetic Engineering, Department of Microbiology and Plant Biology, University of Belgrade, Vojvode Stepe 444a, 11042 Belgrade, Serbia. FAU - Samardzic, Jelena AU - Samardzic J AD - Institute of Molecular Genetics and Genetic Engineering, Department of Microbiology and Plant Biology, University of Belgrade, Vojvode Stepe 444a, 11042 Belgrade, Serbia. LA - eng GR - 451-03-33/2026-03/200042/ministry of science, technological development and innovation of the Republic of Serbia/ PT - Journal Article DEP - 20261018 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708774 OTO - NOTNLM OT - copper stress OT - cucumber OT - miR398-SOD OT - non-coding RNAs OT - silicon OT - transporters COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:44 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - plants15203189 [pii] AID - plants-15-03189 [pii] AID - 10.3390/plants15203189 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 18;15(20):3189. doi: 10.3390/plants15203189. PMID- 42943139 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 17 TI - Cadmium Exposure Modulates Quality of the Medicinal Plant Dendrobium officinale via Regulation of Gene Expression, Antioxidant Systems, Bioactive Compound Accumulation, and Rhizosphere Microbial Community. LID - 10.3390/plants15203187 [doi] LID - 3187 AB - With increasing industrialization, heavy metal contamination poses a growing threat to ecosystem stability and human health. Cadmium (Cd) is taken up by medicinal plants through the soil-plant continuum, altering rhizosphere microbial communities and thereby affecting plant metabolism and bioactive compound accumulation. In this study, Dendrobium officinale Kimura & Migo (D. officinale) was used as a model medicinal plant to investigate the effects of Cd exposure on plant quality and rhizosphere microbial community composition. The results show that different D. officinale strains exhibited significant differences in growth traits and Cd accumulation under Cd exposure. Under the 0.9 mg.kg(-1) Cd treatment, mean Cd concentrations in the strain with the highest Cd accumulation (Strain 2) reached 6.42, 1.08, and 1.70 mg.kg(-1) DW in roots, stems, and leaves, respectively, compared with 2.13, 0.73, and 0.66 mg.kg(-1) DW in the strain with the lowest Cd accumulation (Strain 1), corresponding to approximately 3.0-, 1.5-, and 2.6-fold higher accumulation. Cd exposure was associated with strain-specific changes in metal transport-related gene expression, antioxidant responses, bioactive constituent accumulation, and rhizosphere microbial community composition. Together, these changes highlight the complex relationship between Cd accumulation, plant physiological responses, and medicinal quality. These findings provide a theoretical reference for the selection of elite D. officinale strains and for the safe cultivation of this medicinal plant. FAU - Bao, Hexigeduleng AU - Bao H AUID- ORCID: 0000-0002-7097-8616 AD - Key Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Science, China Jiliang University, Hangzhou 310018, China. AD - College of Smart Agriculture (College of Artificial Intelligence), Nanjing Agricultural University, Nanjing 210031, China. FAU - Wang, Yu AU - Wang Y AD - Key Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Science, China Jiliang University, Hangzhou 310018, China. FAU - Bao, Hainan AU - Bao H AD - Key Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Science, China Jiliang University, Hangzhou 310018, China. FAU - Wang, Feijuan AU - Wang F AUID- ORCID: 0000-0002-8087-1089 AD - Key Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Science, China Jiliang University, Hangzhou 310018, China. FAU - Jiang, Qiong AU - Jiang Q AD - Key Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Science, China Jiliang University, Hangzhou 310018, China. FAU - Li, Hua AU - Li H AUID- ORCID: 0000-0002-3267-6393 AD - College of Smart Agriculture (College of Artificial Intelligence), Nanjing Agricultural University, Nanjing 210031, China. FAU - Ding, Yanfei AU - Ding Y AD - Key Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Science, China Jiliang University, Hangzhou 310018, China. FAU - Zhu, Cheng AU - Zhu C AD - Key Laboratory of Specialty Agri-Product Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Science, China Jiliang University, Hangzhou 310018, China. LA - eng GR - 2024YW54/Department of Education of Zhejiang Province/ PT - Journal Article DEP - 20261017 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708823 OTO - NOTNLM OT - Dendrobium officinale OT - bioactive compounds OT - cadmium OT - heavy metal pollution OT - medicinal plants OT - rhizosphere microbial community COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:44 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - plants15203187 [pii] AID - plants-15-03187 [pii] AID - 10.3390/plants15203187 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 17;15(20):3187. doi: 10.3390/plants15203187. PMID- 42943138 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 17 TI - Establishment of Positional Signals in Megaspore Mother Cell Specification: An Updated Review. LID - 10.3390/plants15203184 [doi] LID - 3184 AB - The megaspore mother cell (MMC), the initial cell of the female germline in flowering plants, differentiates from a distal subepidermal somatic cell in the ovule. Positional signals guide this process, yet the mechanisms by which multiple signals integrate to specify a single MMC remain poorly understood. Here, we summarize recent advances in MMC specification, focusing on the interplay between positional signals and positional competence. Positional signals include phytohormone gradients, mobile cues, cell wall-derived physical boundaries, and mechanical signals. Positional competence is the ability of a cell to interpret these signals. We propose an integrated model in which chemical gradients define the potential reproductive region, mobile signals establish inhibitory fields, the cell wall gates signal movement, and the cytoskeleton coordinates signal delivery, division-plane patterning, and mechanical transduction. This review provides a conceptual basis for understanding how a single somatic cell is selected to acquire MMC identity and highlights potential targets for further research and crop improvement. FAU - Sun, Ling AU - Sun L AD - Haixia Institute of Science and Technology, School of Future Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Liu, Tingyu AU - Liu T AD - Haixia Institute of Science and Technology, School of Future Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Chen, Yufei AU - Chen Y AD - Haixia Institute of Science and Technology, School of Future Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Cai, Hanyang AU - Cai H AD - Haixia Institute of Science and Technology, School of Future Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Qin, Yuan AU - Qin Y AD - Haixia Institute of Science and Technology, School of Future Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. LA - eng GR - 32270366/National Natural Science Foundation of China/ GR - 32370377/National Natural Science Foundation of China/ GR - 32570412/National Natural Science Foundation of China/ PT - Journal Article PT - Review DEP - 20261017 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13709068 OTO - NOTNLM OT - MMC specification OT - cytoskeleton components OT - mobile signals OT - phytohormone gradients OT - positional competence OT - positional signals COIS- The authors declare no competing interests. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:44 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - plants15203184 [pii] AID - plants-15-03184 [pii] AID - 10.3390/plants15203184 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 17;15(20):3184. doi: 10.3390/plants15203184. PMID- 42943129 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - In-Depth Analysis of the Essential Oil of Achillea erba-rotta subsp. moschata (Asteraceae). LID - 10.3390/plants15203176 [doi] LID - 3176 AB - Achillea erba-rotta subsp. moschata (Wulfen) Vacc. (Sin.: A. moschata Wulfen) is a plant belonging to the Asteraceae family and is traditionally used for its medicinal and aromatic properties. Nowadays, it is primarily used as an ingredient of several commercial liqueurs. The essential oil from a representative number of A. erba-rotta subsp. moschata plants collected from the Eastern Italian Alps and cultivated in the same experimental conditions were investigated by GC/FID and GC/MS methods. A great variability among analyzed plant samples was recorded for the essential oil qualitative and quantitative composition. Oxygenated monoterpenes were the main class of detected compounds, of which cis- and trans-thujones (from nil to 85.5% of the total oil), camphor (from 0.2 to 55.6%) and 1,8-cineol (from 0.3 to 19.7%) were the most abundant. Among sesquiterpenes, the group of caryophyllenes, including caryophyllene and its oxygenate derivatives, were quoted from 3.3 to 29.9%, while beta-eudesmol comprised from nil to 16.9% of the total oil. Given the known toxicity of thujones for human consumption, the results of this study are of particular importance for selecting plants with lower thujone content in the framework of a possible breeding program. FAU - Tava, Aldo AU - Tava A AUID- ORCID: 0000-0003-4657-4053 AD - Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria (CREA), Centro di ricerca Zootecnia e Acquacultura, Viale Piacenza 29, 26900 Lodi, Italy. FAU - Biazzi, Elisa AU - Biazzi E AUID- ORCID: 0000-0002-1348-8709 AD - Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria (CREA), Centro di ricerca Zootecnia e Acquacultura, Viale Piacenza 29, 26900 Lodi, Italy. FAU - Fusani, Pietro AU - Fusani P AUID- ORCID: 0000-0001-8643-8414 AD - Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria (CREA), Centro di ricerca Foreste e Legno, Piazza Nicolini 6, 38100 Trento, Italy. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13709309 OTO - NOTNLM OT - camphor OT - caryophyllene derivatives OT - essential oil composition OT - thujones OT - variability COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:44 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - plants15203176 [pii] AID - plants-15-03176 [pii] AID - 10.3390/plants15203176 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 16;15(20):3176. doi: 10.3390/plants15203176. PMID- 42943125 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - Integrating Abiotic Stress Adaptation and Disease Resistance in Climate-Resilient Crops: Pangenomics, Genomic Selection, and AI-Driven Breeding. LID - 10.3390/plants15203171 [doi] LID - 3171 AB - Climate change is increasing the frequency and intensity of abiotic stresses, including drought, high temperature, salinity, and waterlogging, which not only directly constrain crop growth and productivity but also reshape plant-pathogen interactions by altering host physiology and immune responses. These climate-driven environmental pressures can accelerate pathogen spread and generational turnover while compromising crop disease resistance through complex interactions between abiotic stress responses and plant immunity. Concurrently, modern cultivars have lost a substantial portion of their genetic diversity through prolonged productivity-focused selection, limiting their capacity to respond to rapidly changing environmental and disease pressures. Consequently, resistance strategies relying primarily on a few major resistance genes and Marker-Assisted Selection (MAS) are becoming increasingly insufficient for maintaining stable crop performance under climate change. This review discusses next-generation strategies for developing climate-resilient disease-resistant crops through three complementary approaches. First, we examine quantitative disease resistance (QDR), which is typically governed by multiple loci with small to moderate effects and can contribute to durable resistance, although its effectiveness depends on the host genotype, pathogen isolate, and environment. We also evaluate the application of genomic selection (GS) to predict quantitative resistance and select individuals with favorable genomic estimated breeding values across major crops. Third, we highlight AI-driven precision breeding that integrates genomic, transcriptomic, metabolomic, phenomic, and environmental information to decipher complex genotype-by-environment (G x E) interactions and improve the prediction of stress-associated disease resistance. Collectively, integrating genetic diversity, genome-wide prediction, multi-omics, and AI provides a framework for breeding toward durable disease resistance and stable productivity under increasingly complex abiotic stress environments, with resistance performance requiring validation across relevant pathogen isolates and environments. FAU - Hwang, Yousol AU - Hwang Y AUID- ORCID: 0009-0000-7718-0781 AD - Department of Crop Science, Chungbuk National University, Cheongju 28644, Republic of Korea. FAU - Ryu, Gwangmin AU - Ryu G AD - Department of Crop Science, Chungbuk National University, Cheongju 28644, Republic of Korea. FAU - Lee, Moon-Sub AU - Lee MS AD - Department of Crop Science, Chungbuk National University, Cheongju 28644, Republic of Korea. FAU - So, Yoon-Sup AU - So YS AD - Department of Crop Science, Chungbuk National University, Cheongju 28644, Republic of Korea. FAU - Yu, Ju-Kyung AU - Yu JK AD - Department of Crop Science, Chungbuk National University, Cheongju 28644, Republic of Korea. FAU - Mun, Bong-Gyu AU - Mun BG AUID- ORCID: 0000-0002-6670-1315 AD - Department of Environmental and Biological Chemistry, Chungbuk National University, Cheongju 28644, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708963 OTO - NOTNLM OT - artificial intelligence OT - climate change OT - crop disease resistance breeding OT - genomic selection OT - multi-omics OT - pangenome OT - quantitative disease resistance COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:44 PHST- 2026/08/26 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - plants15203171 [pii] AID - plants-15-03171 [pii] AID - 10.3390/plants15203171 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 16;15(20):3171. doi: 10.3390/plants15203171. PMID- 42943123 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - Limits to Plant Species' Ranges: A Unified Eco-Evolutionary Model and Molecular Mechanisms. LID - 10.3390/plants15203170 [doi] LID - 3170 AB - The formation of a species' range generally involves dispersal and selection processes where dispersal enhancing species expansion is counterbalanced by selection that reduces mean population fitness at the edge of a species' range. The application of high-throughput sequencing has made it possible to identify the genetic basis for plant adaptation, which allows for assessing molecular mechanisms of genetic regulation at the edge. Here, we first review the theoretical advances in studying a species' range based on a unified eco-evolutionary model. Genetic loads generated by different evolutionary processes can be uniformly expressed in a general genetic component of regulation (r g). We then discuss the molecular basis of adaptation from recent empirical studies, including SNP-based detection of adaptation signatures, the regulation of gene expression, genomic structural variation, transposable elements, gene family expansion/contraction, and genome size. We also discuss the potential of applying estimates of molecular evolutionary rates (K(a)/K(s)) to infer plant adaptation in the edge populations by comparing the differences between the central and edge populations or between the central and edge communities. Finally, we discuss how plant mating systems regulate genetic loads in edge populations and alter the relative genetic loads at the gametophyte versus sporophyte phase. By linking the eco-evolutionary model with genomic evidence, this review explores several issues to incorporate genetic and ecological regulations and genomic variation into studies on a species' range. Addressing these issues can extend our understanding of the limits to a plant species' range. FAU - Lv, Yan-Wen AU - Lv YW AD - College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China. AD - Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, China. FAU - Liu, Xiao-Han AU - Liu XH AD - College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China. AD - Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, China. FAU - Xie, Hui AU - Xie H AD - College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China. AD - Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, China. FAU - Bai, Yi-Lan AU - Bai YL AD - College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China. AD - Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, China. FAU - Gao, Zi-Yi AU - Gao ZY AD - College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China. AD - Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, China. FAU - Hu, Xin-Sheng AU - Hu XS AUID- ORCID: 0000-0002-8049-9491 AD - College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China. AD - Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, China. LA - eng GR - 32171819/National Natural Science Foundation of China/ GR - 4400-K16013/South China Agricultural University/ PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13709127 OTO - NOTNLM OT - eco-evolutionary model OT - genomic structural variation OT - landscape genomics OT - mating system OT - range limits COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analysis, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:44 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - plants15203170 [pii] AID - plants-15-03170 [pii] AID - 10.3390/plants15203170 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 16;15(20):3170. doi: 10.3390/plants15203170. PMID- 42943122 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - Genome-Wide Identification and Expression Analysis of Aquaporin Gene Family in 84K Poplar. LID - 10.3390/plants15203167 [doi] LID - 3167 AB - Aquaporins (AQPs) mediate the transmembrane transport of water and diverse small molecules, playing essential roles in plant growth and development and stress responses. However, their genome-wide organization and evolution at the subgenome level in hybrid 84K poplar (Populus alba x Populus tremula var. glandulosa) remain incompletely characterized. Based on the telomere-to-telomere genome of 84K poplar, we identified 94 PagAQPs, with 46 and 48 members in the A and G subgenomes, respectively. Phylogenetic analysis classified them into five subfamilies unevenly distributed across 29 chromosomes, with PagPIP and PagTIP being the two largest subfamilies. Most PagAQPs were retained as corresponding A/G allelic pairs, whereas few exhibit subgenome-specific retention. Valid Ka/Ks ratios for 38 allelic pairs and 59 paralogous gene pairs were all below 1, indicating predominant purifying selection. Gene structure, conserved motifs, and substrate-recognition residues showed conservation within subfamilies and divergence among subfamilies. The promoters contained predicted cis-acting elements associated with light, phytohormone and abiotic stress responses. Expression profiling showed that some PagPIP and PagTIP members were highly expressed in vascular tissues and exhibited changes in transcript abundance under nitrogen and drought treatments. Compared with wild-type plants, the 35S:PagPIP2;6A overexpression lines were shorter before treatment and exhibited higher leaf relative water content and lower malondialdehyde content after water withholding. This study systematically characterized the subgenomic distribution, evolutionary features and expression patterns of the PagAQP family in 84K poplar and identified candidate genes for further functional investigation. FAU - Zhu, Mingqiang AU - Zhu M AD - Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Chongqing Technology Innovation Center of Breeding, Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, School of Life Sciences, Southwest University, Chongqing 400715, China. FAU - Wang, Kaiqi AU - Wang K AD - Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Chongqing Technology Innovation Center of Breeding, Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, School of Life Sciences, Southwest University, Chongqing 400715, China. FAU - Wang, Jiyu AU - Wang J AD - Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Chongqing Technology Innovation Center of Breeding, Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, School of Life Sciences, Southwest University, Chongqing 400715, China. FAU - Sun, Kuan AU - Sun K AD - Chongqing Nanshan Botanical Garden, Chongqing 400715, China. FAU - Wang, Haiyang AU - Wang H AD - Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Chongqing Technology Innovation Center of Breeding, Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, School of Life Sciences, Southwest University, Chongqing 400715, China. FAU - Li, Yunfeng AU - Li Y AD - Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Chongqing Technology Innovation Center of Breeding, Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, School of Life Sciences, Southwest University, Chongqing 400715, China. FAU - Wang, Jin AU - Wang J AD - Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Chongqing Technology Innovation Center of Breeding, Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, School of Life Sciences, Southwest University, Chongqing 400715, China. FAU - Zhu, Shunqin AU - Zhu S AD - Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Chongqing Technology Innovation Center of Breeding, Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, School of Life Sciences, Southwest University, Chongqing 400715, China. AD - Key Laboratory of Eco-Environments of Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, China. LA - eng GR - 2D-2022-2/Chongqing Major Special Project for Promoting Forestry through Science and Technology/ GR - SWU-KR26013/Fundamental Research Funds for the Central Universities/ GR - TGB-KF-2024-007/Open Fund of State Key Laboratory of Tree Genetics and Breeding (Chinese Academy of Forestry)/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13709191 OTO - NOTNLM OT - 84K poplar OT - aquaporin OT - drought stress response OT - expression pattern OT - gene family COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:44 PHST- 2026/07/02 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - plants15203167 [pii] AID - plants-15-03167 [pii] AID - 10.3390/plants15203167 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 16;15(20):3167. doi: 10.3390/plants15203167. PMID- 42943121 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - Multitrait Screening and SSR Fingerprinting of Calligonum Section Medusa Individuals for Desert Ecological Restoration. LID - 10.3390/plants15203169 [doi] LID - 3169 AB - Calligonum sect. Medusa is an important resource for desert ecological restoration, but practical criteria for elite germplasm screening and reliable methods for identity verification remain limited. We evaluated 13 morphological, physiological, and biochemical traits in 121 individuals conserved ex situ at the Turpan Campus of Yili-Tupan National Botanical Garden (electable) using principal component analysis, membership-function evaluation, hierarchical clustering, and stepwise regression; 20 individuals were further genotyped at 13 simple sequence repeat (SSR) loci. Trait coefficients of variation ranged from 6.54% to 53.79%, and five principal components explained 68.40% of the total variation. Five candidate elite individuals (ZLY37, ZLY40, ZLY44, ZLY38, and ZLY01) were identified. A reduced six-trait model retained crown width, predawn water potential, plant height, soluble sugar content, plant-form integrity, and assimilating-branch length, from which preliminary empirical reference thresholds were derived. Of the 13 SSR loci, 10 were polymorphic (mean PIC = 0.357), and a candidate five-locus panel distinguished the same 20 individuals used for its selection and enabled fingerprint construction for the five candidates. This study provides a practical framework for preliminary elite germplasm screening and molecular identity verification in Calligonum sect. Medusa, supporting desert ecological restoration, germplasm management, and subsequent cultivar development. FAU - Du, Mingzhi AU - Du M AD - College of Forestry and Landscape Architecture, Xinjiang Agricultural University, Urumqi 830052, China. AD - State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China. AD - Turpan Eremophytes Botanical Garden, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Turpan 838008, China. AD - China-Tajikistan Belt and Road Joint Laboratory on Biodiversity Conservation and Sustainable Use, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China. FAU - Yao, Yinjuan AU - Yao Y AD - College of Forestry and Landscape Architecture, Xinjiang Agricultural University, Urumqi 830052, China. AD - State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China. AD - Turpan Eremophytes Botanical Garden, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Turpan 838008, China. AD - China-Tajikistan Belt and Road Joint Laboratory on Biodiversity Conservation and Sustainable Use, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China. FAU - Lu, Zhongchen AU - Lu Z AD - State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China. AD - Turpan Eremophytes Botanical Garden, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Turpan 838008, China. AD - China-Tajikistan Belt and Road Joint Laboratory on Biodiversity Conservation and Sustainable Use, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China. FAU - Zhang, Jing AU - Zhang J AD - College of Forestry and Landscape Architecture, Xinjiang Agricultural University, Urumqi 830052, China. FAU - Wang, Xiyong AU - Wang X AD - State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China. AD - Turpan Eremophytes Botanical Garden, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Turpan 838008, China. AD - China-Tajikistan Belt and Road Joint Laboratory on Biodiversity Conservation and Sustainable Use, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China. FAU - Zhang, Jing AU - Zhang J AD - State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China. AD - Turpan Eremophytes Botanical Garden, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Turpan 838008, China. FAU - Wang, Jiancheng AU - Wang J AUID- ORCID: 0000-0002-6851-4951 AD - State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China. AD - Turpan Eremophytes Botanical Garden, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Turpan 838008, China. AD - China-Tajikistan Belt and Road Joint Laboratory on Biodiversity Conservation and Sustainable Use, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China. FAU - Shi, Wei AU - Shi W AUID- ORCID: 0000-0003-1422-418X AD - College of Forestry and Landscape Architecture, Xinjiang Agricultural University, Urumqi 830052, China. AD - State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China. AD - Turpan Eremophytes Botanical Garden, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Turpan 838008, China. AD - China-Tajikistan Belt and Road Joint Laboratory on Biodiversity Conservation and Sustainable Use, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China. LA - eng GR - 42077411/National Natural Science Foundation of China/ GR - 2023TSYCCX0024/People's Government of Xinjiang Uygur Autonomous Region/ GR - [2026]363-005/People's Government of Xinjiang Uygur Autonomous Region/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708842 OTO - NOTNLM OT - Calligonum sect. Medusa OT - SSR fingerprinting OT - candidate elite individuals OT - desert ecological restoration OT - multitrait evaluation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:44 PHST- 2026/09/10 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - plants15203169 [pii] AID - plants-15-03169 [pii] AID - 10.3390/plants15203169 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 16;15(20):3169. doi: 10.3390/plants15203169. PMID- 42943117 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Metabolomic Fingerprinting and UHPLC-ESI-HRMS/MS Profiling of Five Medicinal Plants Associated with Wound Care. LID - 10.3390/plants15203164 [doi] LID - 3164 AB - Chronic wounds remain a challenging medical problem due to the involvement of multiple biological processes that can delay normal tissue repair. Medicinal plants may contain diverse bioactive compounds with potential relevance for wound management. However, detailed characterization of their chemical composition is essential for understanding their biological potential. The phytochemical composition of five medicinal plants, Verbena officinalis, Plantago major, Achillea millefolium, Hypericum perforatum, and Calendula officinalis, was investigated using ultra-high-performance liquid chromatography coupled with high-resolution electrospray ionization tandem mass spectrometry (UHPLC-ESI-HRMS/MS). A total of 162 metabolites were identified across the investigated extracts, representing diverse classes including flavonoids, phenolic acids, phenylethanoid glycosides, xanthones, naphthodianthrones, acylphloroglucinols and iridoid glycosides. A comparison with the available literature data and databases confirmed previously reported constituents and revealed 115 unique compounds that had not previously been reported for at least one of the five investigated plant species. The obtained results demonstrate the remarkable chemical diversity of the selected medicinal plants and provide a comprehensive basis for further investigation of their biological activities and potential use in multicomponent herbal mixtures for wound management. FAU - Davkova Stojanovski, Iskra AU - Davkova Stojanovski I AUID- ORCID: 0000-0001-7589-3817 AD - Faculty of Pharmacy, Institute of Pharmacognosy, Ss. Cyril and Methodius University in Skopje, Mother Theresa 47, 1000 Skopje, North Macedonia. FAU - Gasic, Uros AU - Gasic U AUID- ORCID: 0000-0001-5384-8396 AD - Institute for Biological Research "Sinisa Stankovic", National Institute of Republic of Serbia, Department of Plant Physiology, University of Belgrade, 11108 Belgrade, Serbia. FAU - Misic, Danijela AU - Misic D AUID- ORCID: 0000-0002-5419-0773 AD - Institute for Biological Research "Sinisa Stankovic", National Institute of Republic of Serbia, Department of Plant Physiology, University of Belgrade, 11108 Belgrade, Serbia. FAU - Trajkovska Matevska, Ana AU - Trajkovska Matevska A AUID- ORCID: 0000-0001-7541-5735 AD - Faculty of Pharmacy, Institute of Pharmacognosy, Ss. Cyril and Methodius University in Skopje, Mother Theresa 47, 1000 Skopje, North Macedonia. FAU - Stoilkovska Gjorgievska, Veronika AU - Stoilkovska Gjorgievska V AUID- ORCID: 0000-0001-8656-1316 AD - Faculty of Pharmacy, Institute of Pharmacognosy, Ss. Cyril and Methodius University in Skopje, Mother Theresa 47, 1000 Skopje, North Macedonia. FAU - Todorov, Filip AU - Todorov F AUID- ORCID: 0009-0006-0817-4883 AD - Faculty of Pharmacy, Institute of Pharmacognosy, Ss. Cyril and Methodius University in Skopje, Mother Theresa 47, 1000 Skopje, North Macedonia. FAU - Cvetkovikj Karanfilova, Ivana AU - Cvetkovikj Karanfilova I AUID- ORCID: 0000-0002-4248-140X AD - Faculty of Pharmacy, Institute of Pharmacognosy, Ss. Cyril and Methodius University in Skopje, Mother Theresa 47, 1000 Skopje, North Macedonia. FAU - Stefkov, Gjoshe AU - Stefkov G AUID- ORCID: 0000-0002-5801-2931 AD - Faculty of Pharmacy, Institute of Pharmacognosy, Ss. Cyril and Methodius University in Skopje, Mother Theresa 47, 1000 Skopje, North Macedonia. FAU - Karapandzova, Marija AU - Karapandzova M AUID- ORCID: 0000-0002-4596-081X AD - Faculty of Pharmacy, Institute of Pharmacognosy, Ss. Cyril and Methodius University in Skopje, Mother Theresa 47, 1000 Skopje, North Macedonia. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708942 OTO - NOTNLM OT - LC-MS/MS OT - flavonoids OT - herbal drugs OT - iridoids OT - phenolics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:44 PHST- 2026/09/14 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - plants15203164 [pii] AID - plants-15-03164 [pii] AID - 10.3390/plants15203164 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 15;15(20):3164. doi: 10.3390/plants15203164. PMID- 42943109 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Genome-Wide Identification of the Two-Pore Potassium Channel Gene Family in Sugar Beet (Beta vulgaris L.) and Functional Analysis of BvTPK2 in Salt Tolerance. LID - 10.3390/plants15203156 [doi] LID - 3156 AB - Tandem-pore potassium (TPK/KCO) channels play important roles in plant salt tolerance and K(+) homeostasis. However, the TPK/KCO gene family has not been systematically characterized in sugar beet (Beta vulgaris L.), the world's second-largest sugar crop, and its functions in salt-stress responses remain largely unknown. In this study, six TPK/KCO family members were identified from the sugar beet genome and systematically analyzed by bioinformatics and expression analyses. The BvTPK genes were unevenly distributed across three chromosomes, designated BvTPK1-BvTPK6, and contained characteristic Ion_trans_2 and PRK10537 domains. Phylogenetic analysis classified TPK/KCO proteins into three major groups, with BvTPK1, BvTPK2, BvTPK3, and BvTPK6 in Group II, BvTPK5 in Group I, and BvTPK4 in Group III. The promoter regions of BvTPKs contained numerous cis-regulatory elements associated with light, hormone, and stress responses. Transcriptomic and RT-qPCR analyses revealed that several BvTPK genes responded to salt stress, with BvTPK2 showing strong salt-induced expression in leaves. Subcellular localization confirmed that BvTPK2 was localized to the tonoplast. Moreover, heterologous overexpression of BvTPK2 enhanced seed germination and survival, improved plant growth and fresh and dry weights, and maintained better photosynthetic performance in Arabidopsis under salt stress, indicating that BvTPK2 positively contributes to salt tolerance. The overexpressing lines also exhibited reduced Na(+) accumulation, enhanced K(+) retention, and lower Na(+)/K(+) ratios, indicating improved ionic homeostasis under salt stress. Collectively, these findings provide a characterization of the TPK/KCO gene family in sugar beet and identify BvTPK2 as a key candidate gene associated with salt tolerance. FAU - Wang, Shuai AU - Wang S AD - College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019, China. FAU - Cui, Yuzhu AU - Cui Y AD - College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019, China. FAU - Li, Yue AU - Li Y AD - College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019, China. FAU - Wang, Yuxin AU - Wang Y AD - College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019, China. FAU - Sun, Yaqing AU - Sun Y AD - College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019, China. FAU - Li, Guolong AU - Li G AD - College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019, China. FAU - Li, Ningning AU - Li N AUID- ORCID: 0000-0002-2903-4832 AD - College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019, China. FAU - Zhang, Shaoying AU - Zhang S AD - College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019, China. LA - eng GR - 2022JBGS0029/The Open Competition Mechanism to Select the Best Candidates Projects in Inner Mongolia Autonomous Region/ GR - 2026YFHH0122/the Key Rresearch and Development and Achievement Transformation Projects in Inner Mongolia Autonomous Region/ GR - 32560479/National Natural Science Foundation of China/ GR - U25A20671/National Natural Science Foundation of China/ GR - BR250104/Special Program for Enhancing the Research Capabilities of Young Teachers in Inner Mongolia Agricultural University/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13709052 OTO - NOTNLM OT - BvTPK2 OT - salt tolerance OT - sugar beet OT - two-pore K+ channels COIS- The authors declare that they have no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:44 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - plants15203156 [pii] AID - plants-15-03156 [pii] AID - 10.3390/plants15203156 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 15;15(20):3156. doi: 10.3390/plants15203156. PMID- 42943100 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Genotype x Isolate Interactions for Tan Spot Resistance Screening in Mungbean. LID - 10.3390/plants15203148 [doi] LID - 3148 AB - Tan spot, caused by Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff), is an important bacterial disease of mungbean (Vigna radiata) in Australia. A key question for resistance screening is whether the relative resistance or susceptibility of mungbean genotypes depends on the isolate used. We evaluated ten Australian Cff isolates in two studies comprising 30 diverse mungbean genotypes (Study 1) and 12 breeding lines and varieties (Study 2), with two glasshouse trials per study. Primary analyses excluded the non-pathogenic control Cff089. Host genotype, isolate and genotype x isolate interaction affected disease severity in both studies (p < 0.001). Isolate and genotype x isolate interaction accounted for 17.3% and 25.3% of total variance in Study 1, and 17.1% and 53.1% in Study 2, respectively. The rank order of the nine pathogenic isolates was not consistent between studies; only two retained the same rank (Spearman rho = -0.08, p = 0.83). Because the genotype panels did not overlap, this cross-study ranking comparison is descriptive. Genotype AGG325972MUNG had the lowest estimated disease severity in Study 1 (62.1) and showed low mean severity across the eight pathogenic isolates for which observations were available. These results show that isolate choice can alter the apparent resistance of germplasm and therefore influence selection decisions. Screening with multiple characterised Cff isolates should provide a more reliable basis for selecting mungbean material for tan spot resistance. FAU - Alamuru, Krishna Chaitanya AU - Alamuru KC AUID- ORCID: 0000-0002-9744-3136 AD - Centre for Crop Health, School of Science, Engineering and Digital Technologies, University of Southern Queensland, Toowoomba, QLD 4350, Australia. FAU - Knight, Noel L AU - Knight NL AUID- ORCID: 0000-0003-3473-4718 AD - Centre for Crop Health, School of Science, Engineering and Digital Technologies, University of Southern Queensland, Toowoomba, QLD 4350, Australia. FAU - Bottomley, Prue AU - Bottomley P AUID- ORCID: 0009-0008-7812-5600 AD - Centre for Crop Health, School of Science, Engineering and Digital Technologies, University of Southern Queensland, Toowoomba, QLD 4350, Australia. AD - Metagen Pty Ltd., Gatton, QLD 4343, Australia. FAU - Ryan, Merrill AU - Ryan M AD - Queensland Department of Primary Industries, Warwick, QLD 4370, Australia. FAU - Kiss, Levente AU - Kiss L AUID- ORCID: 0000-0002-4785-4308 AD - Centre for Crop Health, School of Science, Engineering and Digital Technologies, University of Southern Queensland, Toowoomba, QLD 4350, Australia. FAU - Martin, Anke AU - Martin A AUID- ORCID: 0000-0003-3572-7931 AD - Centre for Crop Health, School of Science, Engineering and Digital Technologies, University of Southern Queensland, Toowoomba, QLD 4350, Australia. LA - eng GR - BACI38/Broadacre Cropping Initiative, a collaboration between the Queensland Department of Primary Industries and the University of Southern Queensland/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708807 OTO - NOTNLM OT - Curtobacterium flaccumfaciens pv. flaccumfaciens OT - bacterial tan spot OT - disease severity OT - genotype x isolate interaction OT - germplasm screening OT - mungbean OT - pathogen diversity OT - resistance screening COIS- Author Prue Bottomley is currently employed by the company Metagen Pty Ltd. She was employed by the University of Southern Queensland when this research was conducted, and Metagen Pty Ltd. had no role in the study. All authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:44 PHST- 2026/08/26 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - plants15203148 [pii] AID - plants-15-03148 [pii] AID - 10.3390/plants15203148 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 15;15(20):3148. doi: 10.3390/plants15203148. PMID- 42943099 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Multi-Trait Phenotypic and SSR-Based Genetic Diversity Assessment of Individual Plants for Parental Selection in Turf-Type Tall Fescue. LID - 10.3390/plants15203146 [doi] LID - 3146 AB - Climate change has increased the need for turfgrass germplasm with improved adaptation, persistence, and resource-use efficiency. Tall fescue (Schedonorus arundinaceus) is an important cool-season turfgrass species valued for its tolerance to environmental stresses. This study aimed to assess phenotypic and molecular diversity among individual tall fescue plants derived from 24 United States Department of Agriculture (USDA) Gene Bank accessions and to identify promising turf-type parental material by integrating phenotypic and molecular information. A total of 223 genotypes were evaluated for 17 morphological and agronomic traits, and 120 genotypes were selected using a multi-trait weighted selection index for SSR-based molecular characterization. Principal component (PC) analysis showed that PC1 and PC2 accounted for 50.8% and 12.0% of the total phenotypic variation, respectively. Fourteen polymorphic SSR markers generated 69 polymorphic bands, with an overall mean polymorphism information content (PIC) value of 0.343. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) analysis resolved two major molecular groups with four nested subclusters. Mantel analysis showed a weak but significant positive association between phenotypic and SSR-based molecular distances (r = 0.137, p < 0.001), indicating limited correspondence between the two data types. By jointly considering morphological performance and molecular diversity, 60 genotypes were identified as promising parental candidates for future tall fescue breeding programs. FAU - Zere Taskin, Sinem AU - Zere Taskin S AUID- ORCID: 0000-0002-2243-2993 AD - Department of Field Crops, Bursa Uludag University, 16059 Bursa, Turkiye. FAU - Yonter, Fikret AU - Yonter F AUID- ORCID: 0000-0002-4813-1611 AD - Yildiz Plantbased Products Seeds and Agriculture Ind. Co., 59100 Istanbul, Turkiye. FAU - Teoman Duran, Sevin AU - Teoman Duran S AUID- ORCID: 0000-0003-1469-6777 AD - Organic Agriculture Program, Department of Crop and Animal Production, Karacabey Vocational School, Bursa Uludag University, 16700 Bursa, Turkiye. FAU - Ipek, Meryem AU - Ipek M AD - Department of Horticulture, Bursa Uludag University, 16059 Bursa, Turkiye. FAU - Bilgili, Ugur AU - Bilgili U AD - Department of Field Crops, Bursa Uludag University, 16059 Bursa, Turkiye. LA - eng GR - 124O840/The Scientific and Technological Research Council of Turkiye/ GR - FGA-2023-1564/Bursa Uludag University/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708742 OTO - NOTNLM OT - SSR markers OT - Schedonorus arundinaceus OT - genetic diversity OT - parental selection OT - turfgrass breeding COIS- Author Fikret Yonter was employed by the company Yildiz Plantbased Products Seeds and Agriculture Ind. Co. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:44 PHST- 2026/09/25 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - plants15203146 [pii] AID - plants-15-03146 [pii] AID - 10.3390/plants15203146 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 14;15(20):3146. doi: 10.3390/plants15203146. PMID- 42943098 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Combining Ability and Hybrid Performance of Cowpea Landraces Under Semiarid Conditions. LID - 10.3390/plants15203145 [doi] LID - 3145 AB - Cowpea (Vigna unguiculata (L.) Walp.) plays a key role in food security and income generation in semiarid regions, particularly for smallholder farming systems in the Brazilian semiarid. However, productivity remains low because of environmental limitations, genetic constraints, and traditional crop management practices. We evaluated diallel crosses among cowpea landraces to identify superior parents and cross combinations in terms of earliness and grain yield under semiarid conditions. Six landrace accessions, previously selected through participatory prospecting for adaptability and yield stability, were crossed in a complete diallel design to produce 15 F(1) hybrids and their reciprocal crosses. The hybrids were evaluated under field conditions in the semiarid region during the 2023 growing season using a randomized complete block design with ten replications. The evaluations were conducted according to each genotype's developmental stage and immature pod formation. Early-maturing varieties were harvested at 60 days, intermediate and late varieties between 65 and 75 days. At physiological maturity, early and intermediate genotypes were harvested at 80 days, while late genotypes were harvested between 85 and 90 days. General combining ability (GCA) and specific combining ability (SCA) were estimated using Griffing's Method I (fixed-effects model), and treatment means were compared using the Scott-Knott test at the 5% significance level. Significant GCA and SCA effects (p < 0.05) were detected for 12 of the 14 evaluated traits, whereas reciprocal effects were significant for seven traits. The hybrid combinations L1 x L4, L1 x L5, and L4 x L5, and their reciprocal crosses, exhibited superior agronomic performance, highlighting their potential as valuable genetic resources for cowpea breeding under semiarid environments. FAU - Silva, Maria Joelma da AU - Silva MJD AD - Department of Plant Science and Environmental Sciences, Center for Agrarian Sciences, Federal University of Paraiba, Campus II, Areia 58397-000, PB, Brazil. FAU - Lima, Samandra Silva de AU - Lima SS AD - Department of Plant Science and Environmental Sciences, Center for Agrarian Sciences, Federal University of Paraiba, Campus II, Areia 58397-000, PB, Brazil. FAU - Silva, Mateus de Souza AU - Silva MS AUID- ORCID: 0009-0001-4261-7370 AD - Department of Plant Science and Environmental Sciences, Center for Agrarian Sciences, Federal University of Paraiba, Campus II, Areia 58397-000, PB, Brazil. FAU - Silva, Vitor Emanuel Conrado da AU - Silva VECD AUID- ORCID: 0009-0009-0084-4653 AD - Department of Plant Science and Environmental Sciences, Center for Agrarian Sciences, Federal University of Paraiba, Campus II, Areia 58397-000, PB, Brazil. FAU - Silva, Tais Borges da AU - Silva TBD AD - Department of Plant Science and Environmental Sciences, Center for Agrarian Sciences, Federal University of Paraiba, Campus II, Areia 58397-000, PB, Brazil. FAU - Rolim, Rubens Rangel AU - Rolim RR AD - National Institute of Colonization and Agrarian Reform, Joao Pessoa 58031-000, PB, Brazil. FAU - Silva, Joao Henrique Constantino Sales AU - Silva JHCS AUID- ORCID: 0000-0001-6218-5096 AD - Department of Plant Science and Environmental Sciences, Center for Agrarian Sciences, Federal University of Paraiba, Campus II, Areia 58397-000, PB, Brazil. FAU - Araujo, Helder Farias Pereira de AU - Araujo HFP AD - Department of Biosciences, Center for Agrarian Sciences, Federal University of Paraiba, Campus II, Areia 58397-000, PB, Brazil. FAU - Alves, Edna Ursulino AU - Alves EU AUID- ORCID: 0000-0002-7709-3204 AD - Department of Plant Science and Environmental Sciences, Center for Agrarian Sciences, Federal University of Paraiba, Campus II, Areia 58397-000, PB, Brazil. FAU - Araujo, Lenyneves Duarte Alvino de AU - Araujo LDA AUID- ORCID: 0000-0001-9112-6140 AD - Department of Biosciences, Center for Agrarian Sciences, Federal University of Paraiba, Campus II, Areia 58397-000, PB, Brazil. FAU - do Nascimento, Naysa Flavia Ferreira AU - do Nascimento NFF AUID- ORCID: 0000-0002-4857-3874 AD - Department of Plant Science and Environmental Sciences, Center for Agrarian Sciences, Federal University of Paraiba, Campus II, Areia 58397-000, PB, Brazil. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708615 OTO - NOTNLM OT - Caatinga OT - Vigna unguiculata OT - diallel analysis OT - food security OT - participatory breeding COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:44 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - plants15203145 [pii] AID - plants-15-03145 [pii] AID - 10.3390/plants15203145 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 14;15(20):3145. doi: 10.3390/plants15203145. PMID- 42943096 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Physiological and Transcriptomic Insights into Melatonin-Mediated Alleviation of Cadmium Stress in the Endangered Plant Plantago fengdouensis. LID - 10.3390/plants15203143 [doi] LID - 3143 AB - Plantago fengdouensis is an endangered species threatened by habitat loss, population fragmentation, and cadmium (Cd) contamination. This study evaluated the effects of exogenous melatonin (MT) on Cd tolerance from seed germination to the seedling stage. In Petri-dish assays, 50-100 mg L(-1) Cd markedly inhibited germination; 50 mg L(-1) was selected as the lowest concentration producing severe inhibition while retaining sufficient germination for mitigation testing, and 100 muM MT provided the strongest alleviation. In pot experiments, MT promoted seedling growth, shoot and root biomass accumulation, and root and leaf development while reducing Cd accumulation and root-to-shoot translocation. Physiological analyses showed that 200-250 muM MT most effectively alleviated Cd-associated damage by maintaining photosynthesis, enhancing antioxidant and osmotic-adjustment responses, and modulating endogenous hormones. Transcriptome analysis identified 4871 differentially expressed genes (DEGs) between C50M200 and C50M0, with prominent enrichment in plant hormone signaling, cell-wall/plasma-membrane functions, secondary metabolism, and photosynthesis. Exploratory weighted gene co-expression network analysis (WGCNA) further identified treatment-associated modules and candidate hub genes. Overall, the data support coordinated MT-associated responses involving stronger antioxidant defense, reduced Cd accumulation/translocation, and hormone-signaling remodeling. Because transcriptomes were obtained from leaves and WGCNA included nine samples, the inferred molecular mechanisms should be regarded as candidate pathways pending targeted expression and functional validation. FAU - Peng, Yang AU - Peng Y AD - Key Laboratory of Eco-Environment in the Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China. FAU - Wang, Bin-Ru AU - Wang BR AD - Key Laboratory of Eco-Environment in the Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China. FAU - Zhang, Jia-Bin AU - Zhang JB AD - Key Laboratory of Eco-Environment in the Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China. FAU - Zuo, You-Wei AU - Zuo YW AUID- ORCID: 0000-0002-7281-4527 AD - Key Laboratory of Eco-Environment in the Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China. FAU - Liang, Xin AU - Liang X AUID- ORCID: 0009-0005-0659-0544 AD - Key Laboratory of Eco-Environment in the Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China. FAU - Zhou, Sheng-Mao AU - Zhou SM AD - Key Laboratory of Eco-Environment in the Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China. FAU - You, Shi-Qi AU - You SQ AD - Key Laboratory of Eco-Environment in the Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China. FAU - Deng, Hong-Ping AU - Deng HP AD - Key Laboratory of Eco-Environment in the Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Beibei, Chongqing 400715, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13709181 OTO - NOTNLM OT - Plantago fengdouensis OT - cadmium stress OT - endangered plants OT - melatonin OT - physiological characteristics OT - transcriptomics COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:44 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - plants15203143 [pii] AID - plants-15-03143 [pii] AID - 10.3390/plants15203143 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 14;15(20):3143. doi: 10.3390/plants15203143. PMID- 42943085 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Integrated Flame Weeding and Biochar Reutilization Enhance Soybean Yield by Promoting Soil Microbial Abundance. LID - 10.3390/plants15203132 [doi] LID - 3132 AB - Weed interference and crop residue are major constraints on sustainable soybean production, particularly in intensive tropical agriculture. Flame weeding provides a non-chemical approach for weed management, but the agronomic value of carbonized residues generated during this process remains largely unexplored. In this study, flame-weeding temperatures ranging from 300 to 800 degrees C were first screened based on weed carbonization efficiency and air-quality condition, after which 500 and 600 degrees C were selected for field evaluation. A two-season field experiment was conducted using a 3 x 2 treatment structure combining three pre-sowing flame-weeding temperatures (0, 500, and 600 degrees C) with or without biochar from flame-weeding (BFW) foliar spraying. Each treatment included four replicates. Flame weeding was applied before soybean sowing, whereas BFW foliar spraying was performed at the V4, R2, R4, and R6 growth stages. Flame weeding altered the soil environment, increasing electrical conductivity and reducing soil nematode abundance. Combined treatments produced the highest yields in soybean. In particular, T600-BFW increased the yield per plant by 73.32% and 32.43% relative to the CK in the two seasons, respectively. Flame weeding exerted a significant lethal effect on soil nematodes. Soil nematode density declined from 149.75 to 2.92 ind.20 g(-1) of soil at week 10 following T600 treatment. Compared to CK, T600-BFW increased the root nodule number by 31.71%. The quantities of bacteria, fungi, and actinomycetes in T500 and T600 increased significantly after flame weeding. Redundancy analysis identified soil temperature, electrical conductivity, and pH as major variables associated with microbial community variation. The PLS-PM model showed that soil microorganisms exerted a significant positive effect on soybean yield. These findings indicate that flame weeding and BFW reutilization enhance soybean yield through complementary soil-microbe-root and leaf physiological improvement. This integrated strategy provides a potential pathway for simultaneously managing weeds, reutilizing field-derived carbonized biomass, and improving soybean production. FAU - Qu, Ziyang AU - Qu Z AD - Sanya Institute of Nanjing Agricultural University, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. FAU - Cheng, Hongbin AU - Cheng H AD - Hainan State Farms Academy of Sciences Group Co., Ltd., Sanya 572024, China. FAU - Xu, Qiyun AU - Xu Q AD - Hainan State Farms Academy of Sciences Group Co., Ltd., Sanya 572024, China. FAU - Su, Mengru AU - Su M AD - Sanya Institute of Nanjing Agricultural University, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. FAU - Qi, Mingniang AU - Qi M AD - Sanya Institute of Nanjing Agricultural University, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. FAU - Li, Meishuang AU - Li M AUID- ORCID: 0009-0007-0052-1497 AD - Sanya Institute of Nanjing Agricultural University, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. FAU - Chen, Yongkang AU - Chen Y AD - Hainan State Farms Academy of Sciences Group Co., Ltd., Sanya 572024, China. FAU - Jian, Chunping AU - Jian C AD - Hainan State Farms Academy of Sciences Group Co., Ltd., Sanya 572024, China. FAU - Wang, Yongpeng AU - Wang Y AD - Hainan State Farms Academy of Sciences Group Co., Ltd., Sanya 572024, China. FAU - Fan, Xiaorong AU - Fan X AUID- ORCID: 0000-0001-8844-1713 AD - Sanya Institute of Nanjing Agricultural University, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708631 OTO - NOTNLM OT - biochar OT - flame weeding OT - foliar spraying OT - microbial communities OT - physiological improvement OT - root nodulation OT - soil properties OT - soybean COIS- Authors Hongbin Cheng, Qiyun Xu, Yongkang Chen, Chunping Jian, Yongpeng Wang were employed by the company Hainan State Farms Academy of Sciences Group Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:44 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - plants15203132 [pii] AID - plants-15-03132 [pii] AID - 10.3390/plants15203132 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 14;15(20):3132. doi: 10.3390/plants15203132. PMID- 42943084 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Storage-Dependent Responses of KDML 105 Rice (Oryza sativa L.) Seeds Across Dynamic Magnetic-Field Treatment Conditions: Germination, Seedling Growth, and Dual-Phase Hydration Modelling. LID - 10.3390/plants15203131 [doi] LID - 3131 AB - Seed ageing reduces germination and seedling performance, but variation in these responses and hydration kinetics across dynamic magnetic-field exposure (DMFE) conditions is poorly defined. Khao Dawk Mali 105 rice seeds (Oryza sativa L.) stored for 10-13 months were treated for 3, 6, or 9 min at nominal maximum surface flux densities of 150, 270, 370, or 470 mT. Untreated controls were excluded from the factorial analyses and used as descriptive references. Water uptake was modelled kinetically, and germination, vigour, and seedling growth by response surfaces; for early germination the arcsine-square-root scale was adopted after grouped cross-validation. Storage duration dominated every endpoint, especially early germination (eta(p)(2) = 0.991), declining approximately 28 percentage points per month. DMFE-associated differences were concentrated during early imbibition: treated seeds took up 2.9-4.9 percentage points more water than controls at 6 h, narrowing to 0.6-1.2 points by 42 h. The Reduced Dual-Phase Gompertz model was decisively favoured (DeltaAICc = 260.2-347.2). Flux density was retained in the earlier hydration amplitude and all six seedling-growth models, and on the arcsine scale in a shallow early-germination profile whose selection was sensitive to design support and which did not improve cross-validated prediction. Independent seed lots, airflow-only controls, and field validation are required before practical recommendations. FAU - Gaewsondee, Tiwanat AU - Gaewsondee T AUID- ORCID: 0000-0001-9168-4716 AD - Department of Mechanical Engineering, Faculty of Engineering, Nakhon Phanom University, Nakhon Phanom 48000, Thailand. FAU - Khaengkhan, Phirayot AU - Khaengkhan P AD - Department of Agricultural Technology, Faculty of Technology, Mahasarakham University, Maha Sarakham 44150, Thailand. FAU - Chiawchanwattana, Cherdpong AU - Chiawchanwattana C AD - Department of Mechanical Engineering, Faculty of Engineering, Mahasarakham University, Maha Sarakham 44150, Thailand. FAU - Yangyuen, Suphan AU - Yangyuen S AD - Department of Mechanical Engineering, Faculty of Engineering, Mahasarakham University, Maha Sarakham 44150, Thailand. LA - eng GR - Mahasarakham University/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13709179 OTO - NOTNLM OT - Oryza sativa L. OT - dual-phase Gompertz model OT - imbibition kinetics OT - magnetic seed treatment OT - response-surface methodology OT - seed ageing OT - seed vigour COIS- The authors declare no conflicts of interest. The sponsors had no role in the design, execution, interpretation, or writing of the study. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:44 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - plants15203131 [pii] AID - plants-15-03131 [pii] AID - 10.3390/plants15203131 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 13;15(20):3131. doi: 10.3390/plants15203131. PMID- 42943083 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Heat Stress Responses During Grain Filling in Two Korean Wheat Cultivars as Affected by Developmental Stage and Exposure Duration. LID - 10.3390/plants15203130 [doi] LID - 3130 AB - High temperature can impair grain development and storage compound accumulation during wheat grain filling, with responses depending on stress intensity, timing, duration, and cultivar. In this study, Saekeumkang and Hwanggeumal were selected from among major Korean wheat cultivars because they differ in grain quality- and starch-related characteristics, providing a basis for comparing cultivar-specific responses to heat stress during grain filling. Plants were exposed to 25/15, 35/25, and 40/30 degrees C (day/night) temperature regimes for 5 d beginning at 10 or 20 days after heading (DAH), and changes in grain morphology, endosperm and embryo development, 100-grain weight, protein concentration, and storage-related gene expression were evaluated. High temperature altered grain developmental traits and generally reduced 100-grain weight, while the magnitude of these effects differed between cultivars and treatment stages. Grain protein concentration and the expression of starch- and storage protein-related genes also showed cultivar- and stage-dependent responses. In a separate RNA-seq experiment initiated at 10 DAH, plants were exposed to the temperature treatments for either 5 or 10 d, and transcriptomic analysis revealed that heat intensity and exposure duration affected both the number and functional characteristics of differentially expressed genes, particularly those associated with protein folding and quality control, chromatin-related processes, and carbohydrate metabolism. These results indicate that heat responses during wheat grain filling are jointly influenced by developmental stage, exposure duration, and cultivar-specific molecular regulation. FAU - Choi, Ju-Young AU - Choi JY AUID- ORCID: 0009-0004-4504-3298 AD - Winter Crop Research Division, National Institute of Crop and Food Science, Rural Development Administration, Wanju 55365, Republic of Korea. FAU - Choi, Junseok AU - Choi J AUID- ORCID: 0000-0001-8383-4905 AD - Winter Crop Research Division, National Institute of Crop and Food Science, Rural Development Administration, Wanju 55365, Republic of Korea. FAU - Son, Jae-Han AU - Son JH AUID- ORCID: 0000-0003-1570-2411 AD - Winter Crop Research Division, National Institute of Crop and Food Science, Rural Development Administration, Wanju 55365, Republic of Korea. FAU - Kim, Yurim AU - Kim Y AD - Winter Crop Research Division, National Institute of Crop and Food Science, Rural Development Administration, Wanju 55365, Republic of Korea. FAU - Jeong, Han-Yong AU - Jeong HY AD - Winter Crop Research Division, National Institute of Crop and Food Science, Rural Development Administration, Wanju 55365, Republic of Korea. FAU - Park, Jinhee AU - Park J AD - Winter Crop Research Division, National Institute of Crop and Food Science, Rural Development Administration, Wanju 55365, Republic of Korea. FAU - Lee, Goeun AU - Lee G AD - Winter Crop Research Division, National Institute of Crop and Food Science, Rural Development Administration, Wanju 55365, Republic of Korea. FAU - Kim, Sookjin AU - Kim S AD - Winter Crop Research Division, National Institute of Crop and Food Science, Rural Development Administration, Wanju 55365, Republic of Korea. FAU - Kang, Chon-Sik AU - Kang CS AD - Winter Crop Research Division, National Institute of Crop and Food Science, Rural Development Administration, Wanju 55365, Republic of Korea. FAU - Cho, Chuloh AU - Cho C AUID- ORCID: 0000-0001-7997-1544 AD - Winter Crop Research Division, National Institute of Crop and Food Science, Rural Development Administration, Wanju 55365, Republic of Korea. LA - eng GR - PJ01723102/Rural Development Administration/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708808 OTO - NOTNLM OT - RNA-seq OT - endosperm development OT - gene expression OT - protein concentration OT - starch metabolism COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:44 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - plants15203130 [pii] AID - plants-15-03130 [pii] AID - 10.3390/plants15203130 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 13;15(20):3130. doi: 10.3390/plants15203130. PMID- 42943077 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Stable Elemental Fingerprints for Multi-Attribute Classification of Ginseng Using Laser-Induced Breakdown Spectroscopy. LID - 10.3390/plants15203124 [doi] LID - 3124 AB - Laser-induced breakdown spectroscopy (LIBS) was evaluated for qualitative within-batch discrimination of ginseng materials by plant category, geographical origin, and growth year. The datasets comprised 200 spectra from four plant categories, 100 spectra from two origins, and 100 spectra from two growth-year groups; each class contained 50 pellets prepared from one ground-and-sieved powder batch. Four chemometric pipelines were compared using five-fold nested cross-validation repeated five times, with preprocessing, wavelength filtering, and tuning restricted to the training folds. The best mean balanced accuracies were 1.000, 0.982, and 1.000 for plant category, origin, and growth year, respectively. An SNV-ANOVA50 filter pipeline retained balanced accuracies of 1.000, 0.980, and 1.000 with 50 of 12,990 detector channels. Conservative wavelength matching, limited to the nominal 0.09 nm instrumental resolution, yielded tentative Ba, Al, Ca, and Fe candidates; these matches do not establish elemental identity. A full-spectrum plus standard normal variate (SNV) control showed that resistance to multiplicative scaling and additive offsets arose primarily from SNV rather than from wavelength selection. Detector-channel shifts remained damaging, especially for the filtered pipeline. Because class identity is confounded with powder-batch identity, the reported cross-validation quantifies technical pellet-level separability within the measured materials and does not estimate performance across independent biological batches. FAU - Dai, Yujia AU - Dai Y AUID- ORCID: 0000-0002-3452-0835 AD - College of Optical, Mechanical and Electrical Engineering (College of Intelligent Equipment Engineering), Zhejiang A&F University, Hangzhou 311300, China. FAU - Zhang, Yilei AU - Zhang Y AD - College of Optical, Mechanical and Electrical Engineering (College of Intelligent Equipment Engineering), Zhejiang A&F University, Hangzhou 311300, China. FAU - Li, Huangwei AU - Li H AD - College of Optical, Mechanical and Electrical Engineering (College of Intelligent Equipment Engineering), Zhejiang A&F University, Hangzhou 311300, China. FAU - Zhao, Shangyong AU - Zhao S AUID- ORCID: 0000-0002-5808-4118 AD - College of Optical, Mechanical and Electrical Engineering (College of Intelligent Equipment Engineering), Zhejiang A&F University, Hangzhou 311300, China. FAU - Liu, Ziyuan AU - Liu Z AUID- ORCID: 0009-0009-7264-2954 AD - College of Optical, Mechanical and Electrical Engineering (College of Intelligent Equipment Engineering), Zhejiang A&F University, Hangzhou 311300, China. LA - eng GR - ZCLQN25A0408/Zhejiang Provincial Natural Science Foundation/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708676 OTO - NOTNLM OT - filter-based wavelength selection OT - ginseng OT - laser-induced breakdown spectroscopy OT - medicinal plants OT - nested cross-validation OT - proximal sensing OT - standard normal variate OT - technical replicates COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:44 PHST- 2026/10/01 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - plants15203124 [pii] AID - plants-15-03124 [pii] AID - 10.3390/plants15203124 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 13;15(20):3124. doi: 10.3390/plants15203124. PMID- 42943074 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - Diversity and Fungicide Sensitivity of Colletotrichum spp. Causing Apple Bitter Rot and Glomerella Leaf Spot in Northwestern Italy. LID - 10.3390/plants15203121 [doi] LID - 3121 AB - Apple bitter rot (ABR) and Glomerella leaf spot (GLS), caused by Colletotrichum spp., have recently become an increasing concern in most Italian apple-growing areas. Surveys were conducted between 2023 and 2025 in 28 commercial apple orchards in Piedmont, Northwestern Italy, to investigate the occurrence and diversity of Colletotrichum species associated with symptomatic fruits and leaves. A total of 155 isolates were obtained and initially screened using gapdh, while 49 representative isolates were selected for species-level identification through multilocus phylogenetic analyses. Five species belonging to the C. gloeosporioides and C. acutatum species complexes were identified: C. chrysophilum, C. alienum, C. fioriniae, C. godetiae, and C. nymphaeae. Eight representative isolates were evaluated for mycelial growth at different temperatures and pathogenicity on apple fruits and leaves, revealing differences in growth and virulence. All tested isolates were pathogenic and fulfilled Koch's postulates. Isolates were characterized for their in vitro sensitivity to fludioxonil, mefentrifluconazole, and pyraclostrobin. EC(50) values were generally low, although greater variability was observed, particularly within the C. gloeosporioides species complex for pyraclostrobin. This study expands current knowledge of the Colletotrichum species associated with ABR and GLS in apple production and provides relevant biological and fungicide sensitivity data as a reference for future epidemiological investigations and disease management strategies. FAU - Martino, Ilaria AU - Martino I AUID- ORCID: 0000-0001-8045-240X AD - Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Largo P. Braccini 2, 10095 Grugliasco, TO, Italy. AD - AGROINNOVA - Interdepartmental Centre for Innovation in the Agro-Environmental Sector, University of Torino, Largo P. Braccini 2, 10095 Grugliasco, TO, Italy. FAU - Raschio, Eleonora AU - Raschio E AD - Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Largo P. Braccini 2, 10095 Grugliasco, TO, Italy. FAU - Dardani, Greta AU - Dardani G AUID- ORCID: 0000-0002-9146-2168 AD - Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Largo P. Braccini 2, 10095 Grugliasco, TO, Italy. FAU - Pichierri, Giuseppe Massimo AU - Pichierri GM AD - Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Largo P. Braccini 2, 10095 Grugliasco, TO, Italy. FAU - Spadaro, Davide AU - Spadaro D AUID- ORCID: 0000-0001-5207-9345 AD - Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Largo P. Braccini 2, 10095 Grugliasco, TO, Italy. AD - AGROINNOVA - Interdepartmental Centre for Innovation in the Agro-Environmental Sector, University of Torino, Largo P. Braccini 2, 10095 Grugliasco, TO, Italy. FAU - Guarnaccia, Vladimiro AU - Guarnaccia V AUID- ORCID: 0000-0003-3188-7743 AD - Department of Agricultural, Forest and Food Sciences (DISAFA), University of Torino, Largo P. Braccini 2, 10095 Grugliasco, TO, Italy. AD - AGROINNOVA - Interdepartmental Centre for Innovation in the Agro-Environmental Sector, University of Torino, Largo P. Braccini 2, 10095 Grugliasco, TO, Italy. LA - eng GR - European Food Safety Authority/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13709003 OTO - NOTNLM OT - Colletotrichum chrysophilum OT - Malus x domestica OT - fludioxonil OT - mefentrifluconazole OT - pyraclostrobin COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:44 PHST- 2026/09/28 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - plants15203121 [pii] AID - plants-15-03121 [pii] AID - 10.3390/plants15203121 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 12;15(20):3121. doi: 10.3390/plants15203121. PMID- 42943071 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - In Vitro and In Vivo Antiplasmodial Activities, Phytochemical Characterization, and Acute Oral Toxicity of Leaf Extracts of Blumea balsamifera. LID - 10.3390/plants15203118 [doi] LID - 3118 AB - Blumea balsamifera is traditionally used in Thai herbal remedies for fever management; however, its antiplasmodial potential has not been comprehensively investigated. In this study, we evaluated the in vitro and in vivo antiplasmodial activities, phytochemical profile, and acute oral toxicity of B. balsamifera leaf extracts. Antiplasmodial activity against Plasmodium falciparum K1 and cytotoxicity against Vero cells were determined using the parasite lactate dehydrogenase (pLDH) assay and an MTT assay, respectively. Phytochemical constituents of the ethanolic extract were characterized by liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). In vivo antiplasmodial assessment was investigated in Plasmodium berghei ANKA-infected mice using Peters' four-day suppressive test at oral doses of 200, 400, and 600 mg/kg. Acute oral toxicity was evaluated following a single oral dose of 2000 mg/kg. The ethanolic extract exhibited promising in vitro antiplasmodial activity, with an IC(50) value of 2.65 microg/mL and a selectivity index (SI) of 23.03, whereas the aqueous extract was inactive (IC(50) > 40 microg/mL). LC-QTOF-MS analysis of the ethanolic leaf extract tentatively identified 16 compounds in the negative ionization mode and 30 compounds in the positive ionization mode. In the four-day suppressive test, the ethanolic extract demonstrated dose-dependent antiplasmodial activity, with parasitemia suppression of 42.29%, 70.74%, and 76.54% at doses of 200, 400, and 600 mg/kg, respectively. In the acute toxicity study, no mortality, abnormal clinical signs, or significant changes in body weight, food consumption, or water intake were observed. Furthermore, no evidence of hepatotoxicity or nephrotoxicity was detected. The ethanolic leaf extract of B. balsamifera demonstrated antiplasmodial activity in both in vitro and in vivo models, with favorable selectivity and no apparent acute toxicity at the tested dose. These findings support the potential of B. balsamifera as a source of antiplasmodial compounds and provide a basis for further phytochemical isolation and mechanistic studies. FAU - Phuwajaroanpong, Arisara AU - Phuwajaroanpong A AD - Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat 80160, Thailand. AD - Center of Excellence in Tropical Pathobiology, Walailak University, Nakhon Si Thammarat 80160, Thailand. FAU - Punsawad, Chuchard AU - Punsawad C AUID- ORCID: 0000-0003-4826-9474 AD - Center of Excellence in Tropical Pathobiology, Walailak University, Nakhon Si Thammarat 80160, Thailand. AD - Department of Medical Sciences, School of Medicine, Walailak University, Nakhon Si Thammarat 80160, Thailand. FAU - Thanwisate, Tanwarat AU - Thanwisate T AUID- ORCID: 0009-0005-0838-7857 AD - Center of Excellence in Tropical Pathobiology, Walailak University, Nakhon Si Thammarat 80160, Thailand. AD - Department of Medical Sciences, School of Medicine, Walailak University, Nakhon Si Thammarat 80160, Thailand. FAU - Chaniad, Prapaporn AU - Chaniad P AD - Center of Excellence in Tropical Pathobiology, Walailak University, Nakhon Si Thammarat 80160, Thailand. AD - Department of Medical Sciences, School of Medicine, Walailak University, Nakhon Si Thammarat 80160, Thailand. FAU - Konyanee, Atthaphon AU - Konyanee A AUID- ORCID: 0009-0006-8108-2168 AD - Center of Excellence in Tropical Pathobiology, Walailak University, Nakhon Si Thammarat 80160, Thailand. AD - Department of Medical Sciences, School of Medicine, Walailak University, Nakhon Si Thammarat 80160, Thailand. FAU - Boonyoung, Piyakorn AU - Boonyoung P AD - Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand. FAU - Septama, Abdi Wira AU - Septama AW AD - Research Center for Pharmaceutical Ingredient and Traditional Medicine, Cibinong Science Center, National Research and Innovation Agency (BRIN), Cibinong Science Center, Cibinong, Bogor 16915, Indonesia. FAU - Plirat, Walaiporn AU - Plirat W AUID- ORCID: 0000-0003-3782-4807 AD - Center of Excellence in Tropical Pathobiology, Walailak University, Nakhon Si Thammarat 80160, Thailand. AD - Department of Medical Sciences, School of Medicine, Walailak University, Nakhon Si Thammarat 80160, Thailand. LA - eng GR - Contract Number WU69228/Walailak University/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708541 OTO - NOTNLM OT - Blumea balsamifera OT - antiplasmodial activity OT - malaria OT - toxicity COIS- The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:44 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - plants15203118 [pii] AID - plants-15-03118 [pii] AID - 10.3390/plants15203118 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 12;15(20):3118. doi: 10.3390/plants15203118. PMID- 42943070 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - Physiological Responses and Transcriptomic Characteristics of Exogenous Trehalose Alleviating Cadmium Toxicity in Maize Seedlings. LID - 10.3390/plants15203117 [doi] LID - 3117 AB - Cadmium (Cd) contamination poses a severe ecological risk to ecosystems. Trehalose (TR) is a non-reducing disaccharide which can mitigate the adverse effects of heavy metals. The physiological responses and transcriptomic characteristics underlying TR-mediated heavy metal tolerance remain unclear in maize. In this study, maize seedlings were exposed to 200 mg L(-1) CdCl(2).2.5H(2)O with or without 23 mM exogenous TR for 5 days. By phenotypic observation, although TR supplementation could not fully restore maize growth under Cd stress, it significantly alleviated Cd-induced inhibition, increasing shoot and root dry weight by 33.80% and 36.67%, respectively, compared with Cd treatment alone. Exogenous TR also improved the photosynthesis under Cd stress. Cd stress triggered excessive ROS accumulation and membrane lipid peroxidation, whereas trehalose application alleviated oxidative damage by significantly reducing O2-, H(2)O(2), and MDA contents while further enhancing SOD and CAT activities. Compared with Cd treatment, cell-wall fractionation revealed a 20.53% increase in root cellulose under Cd+TR combined treatment. Further, exogenous TR reduced Cd concentrations in roots and shoots by 19% and 33%, respectively. RNA-seq identified enriched GO categories related to cell communication, signal transduction, amino acid transmembrane transport, and membrane components, suggesting possible involvement of these processes in the TR response. These findings collectively demonstrate that exogenous trehalose enhances maize cadmium tolerance through coordinated physiological responses and transcriptional adjustments. FAU - Li, Cong AU - Li C AUID- ORCID: 0000-0003-0777-2559 AD - College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China. AD - Shenyang City Key Laboratory of Maize Genomic Selection Breeding, Shenyang 110866, China. FAU - Zhao, Jintao AU - Zhao J AD - College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China. AD - Shenyang City Key Laboratory of Maize Genomic Selection Breeding, Shenyang 110866, China. FAU - Dong, Xinxin AU - Dong X AD - Shenyang Rural Revitalization and Development Center, Shenyang 110034, China. FAU - Yang, Shuang AU - Yang S AD - College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China. AD - Shenyang City Key Laboratory of Maize Genomic Selection Breeding, Shenyang 110866, China. FAU - Tang, Yijun AU - Tang Y AD - Department of Resources and Environment, Zunyi Normal College, Zunyi 563006, China. FAU - Huang, Wenjia AU - Huang W AD - College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China. AD - Shenyang City Key Laboratory of Maize Genomic Selection Breeding, Shenyang 110866, China. FAU - Chen, Jingwei AU - Chen J AD - College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China. AD - Shenyang City Key Laboratory of Maize Genomic Selection Breeding, Shenyang 110866, China. FAU - Qiao, Qingzhuang AU - Qiao Q AD - College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China. AD - Shenyang City Key Laboratory of Maize Genomic Selection Breeding, Shenyang 110866, China. FAU - Zhu, Jialun AU - Zhu J AUID- ORCID: 0009-0003-8583-4847 AD - College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China. AD - Shenyang City Key Laboratory of Maize Genomic Selection Breeding, Shenyang 110866, China. FAU - Ruan, Yanye AU - Ruan Y AD - College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China. AD - Shenyang City Key Laboratory of Maize Genomic Selection Breeding, Shenyang 110866, China. FAU - Dong, Xiaomei AU - Dong X AUID- ORCID: 0000-0001-5506-4544 AD - College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China. AD - Shenyang City Key Laboratory of Maize Genomic Selection Breeding, Shenyang 110866, China. FAU - Fan, Jinjuan AU - Fan J AD - College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang 110866, China. AD - Shenyang City Key Laboratory of Maize Genomic Selection Breeding, Shenyang 110866, China. LA - eng GR - BS[2022]12/Zunyi Normal College/ GR - 2024JH1/11700007-2-4/Department of Science and Technology of Liaoning Province/ GR - 2023GJ0008/Liaoning University/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708718 OTO - NOTNLM OT - cadmium OT - maize OT - physiological responses OT - transcriptome OT - trehalose COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:44 PHST- 2026/08/07 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - plants15203117 [pii] AID - plants-15-03117 [pii] AID - 10.3390/plants15203117 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 12;15(20):3117. doi: 10.3390/plants15203117. PMID- 42943067 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - Indirect Adventitious Shoot Organogenesis and Plant Regeneration from Solanum nigrum L. LID - 10.3390/plants15203115 [doi] LID - 3115 AB - Solanum nigrum L. plays an important role in anti-tumor activity. Previous studies have reported an imperfect in vitro regeneration system with unclear regeneration processes. This study firstly screened the optimal explant type for regeneration, and leaf explants were finally selected as the experimental materials. On this basis, the effects of plant growth regulator combinations and sucrose concentrations on adventitious shoot induction, shoot elongation and rooting were systematically investigated using leaf explants. The results showed that leaf explants exhibited the highest adventitious shoot induction capacity, with an induction rate of 100.00%, significantly higher than petioles (71.33%) and stem segments (51.67%). Using leaf explants, the optimal Murashige and Skoog (MS) medium for adventitious shoot induction contained 2.0 mg.L(-1) 6-benzyladenine (BA), 0.1 mg.L(-1) alpha-naphthaleneacetic acid (NAA), 30 g.L(-1) sucrose and 0.7% (w/v) agar, achieving a stable adventitious shoot induction rate of 100.00%. The optimal medium for adventitious shoot elongation was MS supplemented with 0.5 mg.L(-1) 6-BA, 0.1 mg.L(-1) NAA, 30 g.L(-1) sucrose and 0.7% (w/v) agar, with an average of 27.67 elongated adventitious shoots per explant. The optimal rooting medium was half-strength MS medium containing 0.1 mg.L(-1) NAA, 30 g.L(-1) sucrose and 0.7% (w/v) agar, with a rooting rate of 96.67%. This study successfully established a complete and efficient tissue culture system for S. nigrum, which will facilitate large-scale propagation of this medicinally valuable plant. FAU - Zhang, Jingjing AU - Zhang J AD - School of Pharmacy, Harbin University of Commerce, Harbin 150076, China. FAU - Guo, Qi AU - Guo Q AD - Institute of Forestry Science of Heilongjiang Province, Harbin 150081, China. FAU - Cui, Xinyu AU - Cui X AUID- ORCID: 0009-0007-2838-5933 AD - State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China. FAU - Sun, Tiantian AU - Sun T AD - State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China. FAU - Fan, Zihan AU - Fan Z AD - State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China. FAU - Ma, Xinting AU - Ma X AD - State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China. FAU - Gao, Jinhui AU - Gao J AUID- ORCID: 0000-0002-9660-7127 AD - Yichun Branch of Heilongjiang Academy of Forestry, Yichun 153000, China. FAU - Yang, Jingli AU - Yang J AUID- ORCID: 0000-0003-0922-5954 AD - State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China. FAU - Zhao, Shicheng AU - Zhao S AD - School of Pharmacy, Harbin University of Commerce, Harbin 150076, China. LA - eng GR - Grant numbers 2019-DS124/Harbin University of Commerce/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708538 OTO - NOTNLM OT - Solanum nigrum L. OT - adventitious shoots OT - plant growth regulators OT - regeneration COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:44 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - plants15203115 [pii] AID - plants-15-03115 [pii] AID - 10.3390/plants15203115 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 12;15(20):3115. doi: 10.3390/plants15203115. PMID- 42943064 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 11 TI - Development of Core KASP Marker Panel for DNA Fingerprinting of Pear (Pyrus spp.) Germplasm Collections. LID - 10.3390/plants15203111 [doi] LID - 3111 AB - Accurate varietal identification remains a persistent issue in pear germplasm conservation, creating obstacles in breeding parent selection and impeding progress in elite cultivar research and cultivation. This study aims to develop a set of highly polymorphic single-nucleotide polymorphism (SNP) markers for pear variety identification. Based on the design criteria for highly polymorphic KASP primers, 34 primer pairs were synthesized, of which 25 produced reliable genotyping results, showing clear separation among different genotypes with minimal missing data. The 269 pear accessions were successfully genotyped, with observed heterozygosity (Ho) ranging from 0.33 to 0.56, polymorphism information content (PIC) values between 0.336 and 0.375, and minor allele frequency (MAF) ranging from 0.31 to 0.50. A total of 257 distinct genotypes were obtained from 269 pear accessions, among which two groups of known bud mutation were consistent, and ten groups of potential synonym accessions were detected. The cluster analysis based on the 25 KASP markers separated the 269 pear accessions into two clusters, which showed a broad association with taxonomic background and origin. This study shows that this CoreSNP-KASP marker set can be used for preliminary molecular screening of pear accessions and provides useful information to support subsequent breeding programs. FAU - Qi, Dan AU - Qi D AD - Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China. AD - Key Laboratory of Germplasm Resources Utilization of Horticultural Crops, Ministry of Agriculture and Rural Affairs, Xingcheng 125100, China. FAU - Tian, Luming AU - Tian L AUID- ORCID: 0009-0007-2801-8762 AD - Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China. AD - Key Laboratory of Germplasm Resources Utilization of Horticultural Crops, Ministry of Agriculture and Rural Affairs, Xingcheng 125100, China. FAU - Huo, Hongliang AU - Huo H AD - Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China. AD - Key Laboratory of Germplasm Resources Utilization of Horticultural Crops, Ministry of Agriculture and Rural Affairs, Xingcheng 125100, China. FAU - Xu, Jiayu AU - Xu J AD - Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China. AD - Key Laboratory of Germplasm Resources Utilization of Horticultural Crops, Ministry of Agriculture and Rural Affairs, Xingcheng 125100, China. FAU - Zhang, Simeng AU - Zhang S AD - Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China. AD - Key Laboratory of Germplasm Resources Utilization of Horticultural Crops, Ministry of Agriculture and Rural Affairs, Xingcheng 125100, China. FAU - Zhang, Ying AU - Zhang Y AD - Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China. AD - Key Laboratory of Germplasm Resources Utilization of Horticultural Crops, Ministry of Agriculture and Rural Affairs, Xingcheng 125100, China. FAU - Liu, Chao AU - Liu C AD - Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China. AD - Key Laboratory of Germplasm Resources Utilization of Horticultural Crops, Ministry of Agriculture and Rural Affairs, Xingcheng 125100, China. FAU - Dong, Xingguang AU - Dong X AD - Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China. AD - Key Laboratory of Germplasm Resources Utilization of Horticultural Crops, Ministry of Agriculture and Rural Affairs, Xingcheng 125100, China. FAU - Cao, Yufen AU - Cao Y AD - Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China. AD - Key Laboratory of Germplasm Resources Utilization of Horticultural Crops, Ministry of Agriculture and Rural Affairs, Xingcheng 125100, China. LA - eng GR - CAAS-ASTIP-2021-RIP-01/Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences/ GR - CARS-28-01/Agriculture Research System of China of MOF and MARA/ PT - Journal Article DEP - 20261011 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13709295 OTO - NOTNLM OT - DNA fingerprint OT - KASP OT - Pyrus OT - genetic characteristics OT - genotype COIS- The authors declare that a granted patent associated with the KASP marker panel reported in this manuscript has been obtained, which constitutes a relevant competing interest. There are no other competing or commercial interests. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/11 CRDT- 2026/10/27 10:44 PHST- 2026/08/05 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - plants15203111 [pii] AID - plants-15-03111 [pii] AID - 10.3390/plants15203111 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 11;15(20):3111. doi: 10.3390/plants15203111. PMID- 42943063 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 11 TI - Cold Tolerance in Alfalfa: Physiological Mechanisms, Regulatory Networks, and Molecular Breeding Prospects. LID - 10.3390/plants15203110 [doi] LID - 3110 AB - Alfalfa (Medicago sativa L.) is a perennial legume forage whose winter survival determines stand persistence, spring green-up, and long-term yield in cold regions. Low-temperature stress causes membrane phase transitions, cellular dehydration, impaired photosynthetic electron transport, and excessive reactive oxygen species (ROS) accumulation. Tolerance depends on coordinated antioxidant protection, osmotic adjustment, membrane stabilization, photosynthetic maintenance, and carbon-nitrogen allocation to the root crown. At the molecular level, this process is mediated by calcium ion signaling, phytohormone pathways, and the cold-responsive ICE-CBF/DREB-COR regulatory cascade. Reported molecular resources include calmodulin-like protein modules (MsCML10 and MsCML50), CBF/DREB factors, autophagy and circadian regulators, sugar-metabolism genes, and genes from yellow-flowered alfalfa. This review integrates low-temperature injury, physiological responses, molecular regulatory networks, evaluation methods, genetic resources, and breeding progress. It emphasizes that evidence strength differs among cultivated-alfalfa functional studies, heterologous validation, and omics-based candidate discovery. Major gaps include inconsistent phenotyping, insufficient root-crown and overwintering-bud characterization, limited validation under natural overwintering, and weak translation of candidate genes into breeding populations. Future research should combine standardized multi-environment phenotyping with multi-omics, functional validation in alfalfa, marker development, genomic selection, and multi-trait improvement of cold tolerance, yield, forage quality, and stress adaptation. FAU - Yang, Weiguang AU - Yang W AUID- ORCID: 0009-0003-9349-5021 AD - College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China. FAU - Shi, Xiaojia AU - Shi X AD - College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China. FAU - Lu, Jinyang AU - Lu J AD - College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China. FAU - Liu, Xiangping AU - Liu X AD - College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing 163319, China. LA - eng PT - Journal Article PT - Review DEP - 20261011 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13709258 OTO - NOTNLM OT - alfalfa OT - cold acclimation OT - freezing tolerance OT - genomic selection OT - phenotyping OT - root crown OT - winter hardiness COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/11 CRDT- 2026/10/27 10:44 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - plants15203110 [pii] AID - plants-15-03110 [pii] AID - 10.3390/plants15203110 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 11;15(20):3110. doi: 10.3390/plants15203110. PMID- 42943058 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 10 TI - Development of an Efficient Agrobacterium-Mediated Transformation Protocol for the Tetraploid Medicinal and Vegetable Species Solanum villosum Mill. LID - 10.3390/plants15203103 [doi] LID - 3103 AB - Genetic transformation is a cornerstone approach for functional genomics and molecular breeding in plants. Solanum villosum Mill. is a tetraploid species of the genus Solanum that possesses both nutritional and medicinal properties. However, its genetic improvement has been constrained by the lack of efficient transformation systems. In this study, a robust protocol was established for shoot regeneration and Agrobacterium-mediated transformation of S. villosum using leaf explants and A. tumefaciens strain EHA105/pBI35S::GFP, which harboring the Neomycin phosphotransferase II (NPT II) selectable marker and the green fluorescence protein (GFP) reporter gene. The results showed that 100% leaf explants produced adventitious shoots on semi-solid full-strength MS medium containing 1.0 mg L(-1) thidiazuron (TDZ,) 0.1 mg L(-1) alpha-naphthaleneacetic acid (NAA), 0.7% agar and 10 mg L(-1) AgNO(3), with an average of 7.8 shoots per explant after four weeks. Furthermore, transformation frequency was 81%, and over 90% of kanamycin-resistant plantlets showed a normal phenotype and stable GFP fluorescence. Moreover, transgene integration was verified by molecular analyses and fluorescence imaging of flowers, fruits, and T1 seedlings. Taken together, these results demonstrate that the tissue culture and transformation system established for S. villosum provides a valuable platform for large-scale propagation, gene function studies, and biotechnological improvement of this species. FAU - Piao, Chun-Lan AU - Piao CL AD - Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, College of Horticulture, Zhejiang A&F University, Hangzhou 311300, China. FAU - Cui, Min-Long AU - Cui ML AD - Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, College of Horticulture, Zhejiang A&F University, Hangzhou 311300, China. LA - eng GR - 2018FR004/Zhejiang A & F University/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708614 OTO - NOTNLM OT - Agrobacterium-mediated transformation OT - Solanum villosum Mill. OT - green fluorescent protein (GFP) OT - in vitro shoot regeneration OT - medicinal and edible plant OT - tetraploid COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:44 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - plants15203103 [pii] AID - plants-15-03103 [pii] AID - 10.3390/plants15203103 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 10;15(20):3103. doi: 10.3390/plants15203103. PMID- 42943055 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 10 TI - Cinnamon (Cinnamomum spp.): Phytochemical Constituents and Biological Potential from a Pharmacological Perspective. LID - 10.3390/plants15203102 [doi] LID - 3102 AB - The genus Cinnamomum (Lauraceae) has a long history of use in traditional medicine and continues to attract scientific interest due to its diverse pharmacological properties. This review summarizes the ethnopharmacological uses of Cinnamomum species, including major investigated species such as C. verum, C. cassia, C. burmannii, C. camphora, and other reported species, plant parts utilized, phytochemical composition, biological activities, underlying mechanisms, and safety considerations. Evidence from the literature indicates that bark and leaves are the plant parts most frequently investigated. The phytochemical profile of Cinnamomum comprises diverse volatile and non-volatile constituents, including bioactive compounds such as cinnamaldehyde, eugenol, cinnamic acid, coumarin, and phenolic compounds, with composition influenced by species, geographical origin, plant part, and extraction method. Chemometric approaches have been applied to characterize metabolite variations and their association with biological activities. Preclinical studies demonstrate antioxidant, anti-inflammatory, antidiabetic, anticancer, antimicrobial, cardioprotective, and neuroprotective effects across several Cinnamomum species. These activities are associated with regulation of oxidative stress, NF-kappaB/AP-1-mediated inflammatory responses, PI3K/AKT-related metabolic regulation, and apoptosis-related mechanisms. Despite these promising findings, substantial variability in plant materials, extraction procedures, chemical standardization, experimental models, dose selection, and safety profiles across different Cinnamomum species limits comparison among studies and clinical translation. Further research integrating phytochemical characterization, metabolomics and chemometrics, standardized bioactivity assays, toxicological evaluation, and clinical studies is required to establish the efficacy, safety, and quality of Cinnamomum species. FAU - Setiawan, Pratama AU - Setiawan P AUID- ORCID: 0009-0001-1791-8235 AD - Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, Indonesia. FAU - Trivadila AU - Trivadila AUID- ORCID: 0000-0003-0939-4635 AD - Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, Indonesia. AD - Tropical Biopharmaca Research Center, International Research Institute of Food, Nutrition, Health, and Halal, IPB University, Bogor 16128, Indonesia. FAU - Syafitri, Utami Dyah AU - Syafitri UD AUID- ORCID: 0000-0003-3116-8909 AD - Tropical Biopharmaca Research Center, International Research Institute of Food, Nutrition, Health, and Halal, IPB University, Bogor 16128, Indonesia. AD - Department of Computational Statistics, School of Data Science, Mathematics, and Informatics, IPB University, Bogor 16680, Indonesia. FAU - Syukur, Muhammad AU - Syukur M AD - Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor 16680, Indonesia. FAU - Kusuma, Wisnu Ananta AU - Kusuma WA AUID- ORCID: 0000-0002-3682-244X AD - Tropical Biopharmaca Research Center, International Research Institute of Food, Nutrition, Health, and Halal, IPB University, Bogor 16128, Indonesia. AD - Division of Computational Intelligence and Optimization, School of Data Science, Mathematics, and Informatics, IPB University, Bogor 16680, Indonesia. FAU - Prayogo, Yanico Hadi AU - Prayogo YH AD - Tropical Biopharmaca Research Center, International Research Institute of Food, Nutrition, Health, and Halal, IPB University, Bogor 16128, Indonesia. AD - Department of Forest Products, Faculty of Forestry and Environment, IPB University, Bogor 16680, Indonesia. FAU - Saputra, Agus AU - Saputra A AUID- ORCID: 0009-0000-3340-5395 AD - Faculty of Medicine and Veterinary Medicine, Nusa Cendana University, Kupang 85001, Indonesia. FAU - Abas, Faridah AU - Abas F AUID- ORCID: 0000-0002-8110-9424 AD - Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia. FAU - Rafi, Mohamad AU - Rafi M AUID- ORCID: 0000-0002-5225-8703 AD - Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Bogor 16680, Indonesia. AD - Tropical Biopharmaca Research Center, International Research Institute of Food, Nutrition, Health, and Halal, IPB University, Bogor 16128, Indonesia. LA - eng GR - 4297/B3/DT.03.08/2025/Lembaga Pengelola Dana Pendidikan/ PT - Journal Article PT - Review DEP - 20261010 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708433 OTO - NOTNLM OT - Cinnamomum OT - bioactive compounds OT - natural products OT - pharmacological activity OT - phytochemicals COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:44 PHST- 2026/08/12 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - plants15203102 [pii] AID - plants-15-03102 [pii] AID - 10.3390/plants15203102 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 10;15(20):3102. doi: 10.3390/plants15203102. PMID- 42943052 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 10 TI - More than Beauty-Edible Flowers of New Rosa x hybrida Genotypes Grown in Vojvodina (Serbia) as a Novel Source of Bioactive Compounds. LID - 10.3390/plants15203099 [doi] LID - 3099 AB - Flowers have a long tradition of use beyond ornamental purposes, including incorporation into numerous food products. Roses stand out for their attractive colors and pleasant floral aromas, yet their nutraceutical potential remains largely underestimated. In this study, rose flowers from five new garden cultivars were evaluated for their potential use as food ingredients. Total phenolic, total flavonoid, total monomeric anthocyanin and vitamin C contents were quantified, along with quantitative analysis of 45 selected compounds by LC-MS-MS. Additionally, in vitro antioxidant, neuroprotective and hypoglycemic activities were assessed. They proved to be rich sources of polyphenols (TPC in the range of 122-240 mg GAE/g de, TFC in the range of 18.1-42.7 mg QE/g de) and vitamin C (0.24-0.31 mg/g fw), exhibiting strong antioxidant and moderate neuroprotective effects. They are weak inhibitors of alpha-amylase and strong inhibitors of alpha-glucosidase (23.7-81.6% inhibition at 2.50 mug/mL), suggesting potential benefits for managing blood sugar levels and metabolic health. Cultivar 'Magic Aroma' demonstrated the greatest potential for further research and incorporation into food products. Given modern consumers' growing interest in natural and functional food ingredients, these findings highlight the untapped potential of rose flowers for innovative, health-promoting culinary uses. FAU - Zivanovic, Nemanja AU - Zivanovic N AUID- ORCID: 0000-0001-7839-2815 AD - Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 3, 21000 Novi Sad, Serbia. FAU - Lesjak, Marija AU - Lesjak M AUID- ORCID: 0000-0001-9764-9903 AD - Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 3, 21000 Novi Sad, Serbia. FAU - Orcic, Dejan AU - Orcic D AUID- ORCID: 0000-0003-3474-1723 AD - Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 3, 21000 Novi Sad, Serbia. FAU - Tanjga, Biljana Bozanic AU - Tanjga BB AUID- ORCID: 0009-0007-7272-6061 AD - Breeding Company 'Pheno Geno Roses', Marsala Tita 75, 23326 Ostojicevo, Serbia. FAU - Ljubojevic, Mirjana AU - Ljubojevic M AUID- ORCID: 0000-0001-6887-2807 AD - Department of Fruit Growing, Viticulture, Horticulture and Landscape Architecture, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovica 8, 21000 Novi Sad, Serbia. FAU - Cukanovic, Jelena AU - Cukanovic J AD - Department of Fruit Growing, Viticulture, Horticulture and Landscape Architecture, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovica 8, 21000 Novi Sad, Serbia. FAU - Simin, Natasa AU - Simin N AUID- ORCID: 0000-0002-3837-453X AD - Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 3, 21000 Novi Sad, Serbia. LA - eng PT - Journal Article DEP - 20261010 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708502 OTO - NOTNLM OT - acetylcholinesterase OT - antioxidants OT - edible flowers OT - polyphenols OT - rose petals OT - alpha-amylase OT - alpha-glucosidase COIS- Authors Nemanja Zivanovic, Natasa Simin, Marija Lesjak, Mirjana Ljubojevic, Jelena Cukanovic and Dejan Orcic are employed at the University of Novi Sad, and declare no conflicts of interest. Author Biljana Bozanic Tanjga is employed by the Breeding Company 'Pheno Geno Roses'. She participated in the plant acquisition and basic determination. She had no influence on the trial design, results acquisition, data processing, or interpretation and delivery of the conclusions. The role of the company was plant material curation. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:44 PHST- 2026/09/03 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - plants15203099 [pii] AID - plants-15-03099 [pii] AID - 10.3390/plants15203099 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 10;15(20):3099. doi: 10.3390/plants15203099. PMID- 42943042 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 9 TI - Interannual and Cultivar-Related Variation in Sunflower Productivity and Seed Quality Under Long-Term Rainfed No-Till Management in the Semi-Arid Dobrogea Region of Romania. LID - 10.3390/plants15203089 [doi] LID - 3089 AB - Seasonal climatic variability represents a major constraint on sunflower (Helianthus annuus L.) production in semi-arid environments, yet interannual and cultivar-related variation under long-term no-till management remains insufficiently characterized. This study evaluated interannual and cultivar-related variation in sunflower productivity and seed quality during three consecutive growing seasons (2023-2025) under rainfed long-term no-till conditions in the Dobrogea Region of Romania. Three commercial hybrids (Averon SU, Demetra, and Helesun) were evaluated in a randomized complete block design with three replications, while agronomic management remained unchanged throughout the experimental period. Agronomic traits, yield components, and seed quality parameters were analyzed using analysis of variance, paired t-tests, Pearson's correlation analysis, and partial redundancy analysis (RDA). Pronounced interannual climatic variability was accompanied by substantial differences in sunflower performance. Mean seed yield reached 2169.06 kg ha(-1) in 2023, declined to 848.08 kg ha(-1) in the drought-affected 2024 season, which had the lowest March-August precipitation total, and partially recovered to 1178.68 kg ha(-1) in 2025. Similar seasonal responses were observed for plant density at harvest, seed weight per capitulum, thousand grain weight, test weight, and number of seeds per capitulum, whereas oil content remained comparatively stable (45.19-46.88%). Analysis of variance showed significant interannual variation across all evaluated traits, while significant cultivar effects were detected for plant density, number of seeds per capitulum, seed weight per capitulum, thousand grain weight, and seed yield. Partial RDA further detected a significant joint cultivar effect across the eight standardized response traits after conditioning on study year and block (F = 11.49, R(2) = 0.53, exact p = 0.027). Under the specific environmental conditions observed during the three study years, interannual variation in sunflower performance was substantial; however, annual climatic conditions were not independently replicated and therefore remain confounded with year. Consequently, the observed relationships describe associations within the 2023-2025 site conditions rather than establishing a general causal ranking of climatic and cultivar effects. The results highlight the importance of improving rainfall-use efficiency and integrating cultivar selection with climate-adaptive agronomic management to increase the resilience of sunflower production in water-limited environments. FAU - Stroe, Traian Ciprian AU - Stroe TC AUID- ORCID: 0009-0004-5869-0355 AD - Department of Agriculture, Doctoral School of Engineering and Management of Plant and Animal Resources (IM-RVA), University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Boulevard, 011464 Bucharest, Romania. AD - Department of Natural Sciences, Faculty of Natural and Agricultural Sciences, Ovidius University of Constanta, 1 University Alley, Campus Building B, 900470 Constanta, Romania. FAU - Becze, Anca AU - Becze A AUID- ORCID: 0000-0003-1418-689X AD - National Institute for Research and Development of Optoelectronics INOE 2000, Research Institute for Analytical Instrumentation, 67 Donath Str., 400293 Cluj-Napoca, Romania. FAU - Miron, Liliana AU - Miron L AUID- ORCID: 0009-0000-2790-3056 AD - Department of Natural Sciences, Faculty of Natural and Agricultural Sciences, Ovidius University of Constanta, 1 University Alley, Campus Building B, 900470 Constanta, Romania. FAU - Panaitescu, Liliana AU - Panaitescu L AUID- ORCID: 0000-0001-7878-9628 AD - Department of Agriculture, Doctoral School of Engineering and Management of Plant and Animal Resources (IM-RVA), University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Boulevard, 011464 Bucharest, Romania. AD - Department of Natural Sciences, Faculty of Natural and Agricultural Sciences, Ovidius University of Constanta, 1 University Alley, Campus Building B, 900470 Constanta, Romania. LA - eng PT - Journal Article DEP - 20261009 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708585 OTO - NOTNLM OT - Helianthus annuus L. OT - climatic variability OT - drought OT - no-till OT - precipitation OT - seed quality OT - seed yield OT - semi-arid environment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:44 PHST- 2026/08/27 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - plants15203089 [pii] AID - plants-15-03089 [pii] AID - 10.3390/plants15203089 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 9;15(20):3089. doi: 10.3390/plants15203089. PMID- 42943039 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 9 TI - Linking Soil Properties to Grain Quality: Individual and Combined Effects of Rice Husk Biochar and Potassium Humate in Paddy Rice Systems. LID - 10.3390/plants15203086 [doi] LID - 3086 AB - The integrated effects of biochar and humate on soil nutrient status, shoot nutrient accumulation, and grain composition and quality remain underexplored. This field study evaluated four treatments-control, rice husk biochar (RHB; 15 t/ha), potassium humate (KH; 6.3 kg/ha), and RHB + KH-on soil properties, shoot nutrient accumulation, grain nutrient composition, yield, and grain quality in a premium Thai rice production system. Compared with the control, RHB significantly increased soil pH and shoot accumulation of nitrogen (N; +93%), phosphorus (P; +321%), and potassium (K; +75%) and resulted in a greater grain P concentration and milled grain yield (+56%). RHB also increased volumetric expansion (+42%), with a significant RHB main effect detected in the factorial analysis. KH significantly increased soil total N (+11%) and ammonium (+19%), shoot N (+79%) and P (+363%) accumulation, and grain N concentration relative to the control, while also increasing milled grain yield (+144%) and grain protein content. KH also produced numerical increases in volumetric expansion (+21%) and grain elongation (+4%), although these responses were not significant in the factorial analysis. The RHB + KH treatment significantly increased soil total N (+18%), shoot N (+70%) and P (+423%) accumulation, and the grain K concentration relative to the control and produced treatment-specific changes in the amino acid profile. The amendment treatments generally decreased residual soil nitrate and available P concentrations, although the factorial response of nitrate included a significant RHB effect and RHB x KH interaction but no significant KH main effect. Grain carbohydrate content decreased under the amendment treatments, whereas TPC showed a treatment-specific response characterized by a significant KH effect and RHB x KH interaction. Principal component analysis revealed associations among soil properties, shoot nutrient accumulation, grain nutrient composition, yield, and grain quality attributes. These findings demonstrate distinct effects of RHB and KH, individually and in combination, on soil properties, shoot nutrient accumulation, and grain composition and quality. Overall, the amendments showed potential for modifying soil nutrient dynamics and shoot nutrient accumulation while altering selected grain nutritional and market-relevant quality attributes, although responses varied among individual parameters. FAU - Rupngam, Thidarat AU - Rupngam T AUID- ORCID: 0000-0002-7321-5710 AD - Department of Environmental Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. AD - Office of Research Administration, Chiang Mai University, Chiang Mai 50200, Thailand. FAU - Udomkun, Patchimaporn AU - Udomkun P AUID- ORCID: 0000-0002-3777-9252 AD - Department of Environmental Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. AD - Office of Research Administration, Chiang Mai University, Chiang Mai 50200, Thailand. FAU - Muller, Joachim AU - Muller J AUID- ORCID: 0000-0003-4623-5879 AD - Institute of Agricultural Engineering, Tropics and Subtropics Group, University of Hohenheim, 70599 Stuttgart, Germany. FAU - Boonupara, Thirasant AU - Boonupara T AUID- ORCID: 0000-0001-6135-7714 AD - Living Soil Co., Ltd., Chiang Mai 50100, Thailand. FAU - Kaewlom, Puangrat AU - Kaewlom P AUID- ORCID: 0000-0001-6651-3683 AD - Department of Environmental Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai 50200, Thailand. LA - eng GR - Fundamental Fund/Chiang Mai University/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708574 OTO - NOTNLM OT - plant nutrient uptake OT - rice grain bioactive compounds OT - rice grain characteristics OT - rice grain nutrition OT - soil properties COIS- Author Thirasant Boonupara was employed by the company Living Soil Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:44 PHST- 2026/08/11 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - plants15203086 [pii] AID - plants-15-03086 [pii] AID - 10.3390/plants15203086 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 9;15(20):3086. doi: 10.3390/plants15203086. PMID- 42943038 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 9 TI - Soil Phosphorus Conditions Drives Arbuscular Mycorrhizal Fungal Community Differentiation and Modulate the Growth of Cenchrus pauciflorus. LID - 10.3390/plants15203085 [doi] LID - 3085 AB - Cenchrus pauciflorus is a major invasive plant in the sandy lands of northern China, with the Horqin Sandy Land being a hotspot of its invasion, where soil phosphorus availability may influence its spread through arbuscular mycorrhizal fungi (AMF). To elucidate the effects of soil phosphorus on AMF communities and their role in the invasion of C. pauciflorus, we conducted a field survey at two sites with contrasting phosphorus backgrounds: Chajintai Ranch (high phosphorus) and Bahuta Sumu (low phosphorus). Rhizosphere soil and root samples of C. pauciflorus and the co-occurring native grass Setaria viridis were collected for high-throughput sequencing of 18S SSU rRNA gene to analyze AMF community composition and diversity. Two AMF species, Funneliformis mosseae and Diversispora epigaea, were selected for a pot experiment with C. pauciflorus. Five phosphorus treatments were established under high- and low-phosphorus conditions using different phosphorus forms. The effects of the two AMF species on the growth and reproduction of C. pauciflorus under different phosphorus treatments were analyzed. The results showed that Glomeraceae and Glomus were the predominant family and genus, respectively. Under Bahuta Sumu, Diversispora was relatively enriched in the roots of C. pauciflorus compared with S. viridis roots. However, other nutrient factors also varied between the two field sites, the independent effect of phosphorus on AMF community composition and diversity could not be fully disentangled. The pot experiment revealed that AMF inoculation did not significantly promote the growth of C. pauciflorus under high-phosphorus treatment. In contrast, when phosphorus was low and supplied in insoluble forms, both AMF species significantly promoted the growth and reproduction of C. pauciflorus. D. epigaea exhibited a stronger growth-promoting effect than F. mosseae, with significantly higher colonization rates in most treatments. In summary, C. pauciflorus is associated with the enrichment of specific AMF taxa in low-phosphorus habitats, which may in turn promote its growth by enhancing the acquisition of insoluble phosphorus. This may represent an important belowground symbiotic mechanism underlying its successful invasion in nutrient-poor sandy habitats. FAU - Su, Xun AU - Su X AD - College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao 028000, China. FAU - Ma, Wenjing AU - Ma W AD - College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao 028000, China. FAU - Lyu, Guangchao AU - Lyu G AD - College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao 028000, China. FAU - Xiao, Zhichao AU - Xiao Z AD - College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao 028000, China. FAU - Qi, Yi AU - Qi Y AD - College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao 028000, China. FAU - Li, Jia AU - Li J AD - College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao 028000, China. FAU - Qi, Jiahui AU - Qi J AD - College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao 028000, China. FAU - Chen, Yujie AU - Chen Y AD - College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao 028000, China. FAU - Tian, Xun AU - Tian X AD - College of Life Sciences and Food Engineering, Inner Mongolia Minzu University, Tongliao 028000, China. LA - eng GR - 2026MS0668、2025MS03103、2026ZD025/Natural Science Foundation of Inner Mongolia Autonomous Region/ GR - 32360267、32260683/National Natural Science Foundation of China/ GR - GXKY26Z004/Fundamental Research Funds for the Universities directly under Inner Mongolia Autonomous Region/ GR - BS673、BS615/Inner Mongolia Minzu University Research Startup Fund/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708673 OTO - NOTNLM OT - Cenchrus pauciflorus OT - arbuscular mycorrhizal fungi OT - community composition OT - invasion mechanism OT - soil phosphorus OT - symbiotic effect COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:44 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - plants15203085 [pii] AID - plants-15-03085 [pii] AID - 10.3390/plants15203085 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 9;15(20):3085. doi: 10.3390/plants15203085. PMID- 42943037 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 9 TI - Comparative Analysis of GC-MS Chemical Profiles and Multidimensional Biological Activities of Essential Oils from Thirteen Lamiaceae Plants. LID - 10.3390/plants15203084 [doi] LID - 3084 AB - Lamiaceae plants produce essential oils (EOs) with diverse chemical compositions and biological activities. This study investigated the volatile composition and multiple biological activities of EOs from 13 Lamiaceae species. EOs were obtained by hydrodistillation and characterized by gas chromatography-mass spectrometry (GC-MS), followed by hierarchical clustering and principal component analysis. Antioxidant, antibacterial, cytotoxic, and anti-inflammatory activities were evaluated using in vitro and murine models. GC-MS analysis revealed substantial interspecific variation in volatile composition, with Origanum vulgare characterized by carvacrol and limonene, Thymus spp. by thymol, Rosmarinus officinalis by eucalyptol and alpha-pinene, Mentha canadensis by L-menthol, and Perilla frutescens by perillaldehyde. Scutellaria baicalensis, Elsholtzia ciliata, Phlomis mongolica, and Schizonepeta tenuifolia showed distinctive profiles associated with limonene and isopulegone, nepetalactone, pulegone, and anethole and anisaldehyde, respectively. Multivariate analysis further separated the 13 oils into three chemotype groups: a carvacrol-rich Origanum group, a Scutellaria and Thymus group enriched in sesquiterpenes or thymol, and a group comprising the remaining six genera dominated by shared monoterpene hydrocarbons. Most EOs exhibited substantial 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity, with Rosmarinus officinalis and Perilla frutescens showing the strongest activity, with IC(50) values of 2.14 and 2.08 mug/mL, respectively. Origanum vulgare, Thymus citriodorus, Thymus vulgaris, and Thymus mongolicus displayed strong antibacterial activity against five pathogens, with minimum inhibitory concentration (MIC) values ranging from 2 to 8 mug/mL. Cytotoxicity assays showed potent activity against HepG2 and LNCaP cells, with IC(50) values ranging from 0.31 to 0.95 mug/mL. Elsholtzia ciliata and Perilla frutescens exhibited particularly strong potency, with IC(50) values ranging from 0.31 to 0.40 mug/mL. In a murine skin inflammation model, selected EOs significantly reduced inflammation-related markers, including COX-2, TNF-alpha, NF-kappaB, IKK, Akt, and PKC. Correlation analysis identified significant associations of carvacrol, thymol, and beta-linalool with antibacterial activity, whereas no significant associations were observed between individual major constituents and antioxidant or cytotoxic activity after false discovery rate (FDR) correction. Overall, the 13 Lamiaceae EOs displayed distinct chemical profiles and activity patterns, providing a basis for further investigation of their bioactive constituents and potential applications. FAU - Han, Feng AU - Han F AUID- ORCID: 0000-0001-6836-2399 AD - College of Grassland Science, Inner Mongolia Agricultural University, Hohhot 010011, China. AD - Key Laboratory of National Forestry and Grassland Administration on Native Grass Breeding, College of Grassland Science, Inner Mongolia Agricultural University, Hohhot 010018, China. FAU - Ma, Yingmei AU - Ma Y AD - College of Desert Management, Inner Mongolia Agricultural University, Hohhot 010018, China. FAU - Bai, Yuting AU - Bai Y AUID- ORCID: 0000-0002-7775-1254 AD - College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China. FAU - Zhang, Shuangyi AU - Zhang S AD - College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010011, China. FAU - Zhao, Yan AU - Zhao Y AD - College of Grassland Science, Inner Mongolia Agricultural University, Hohhot 010011, China. AD - Key Laboratory of National Forestry and Grassland Administration on Native Grass Breeding, College of Grassland Science, Inner Mongolia Agricultural University, Hohhot 010018, China. FAU - Yin, Xiujie AU - Yin X AUID- ORCID: 0000-0003-0367-3705 AD - College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China. LA - eng GR - 2025MS03115/Natural Science Foundation Project of Inner Mongolia Autonomous Region/ GR - 2025ZY0117-1/Central Government's Fund for Guiding Local Science and Technology Development/ GR - ZL2024C018/Natural Science Foundation Project of Heilongjiang Province/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708472 OTO - NOTNLM OT - Lamiaceae OT - anti-inflammatory OT - antibacterial OT - antioxidant OT - chemical composition OT - cytotoxicity OT - essential oil COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:44 PHST- 2026/08/18 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - plants15203084 [pii] AID - plants-15-03084 [pii] AID - 10.3390/plants15203084 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 9;15(20):3084. doi: 10.3390/plants15203084. PMID- 42943036 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 9 TI - Effects of Different Ecological Restoration Treatments on Plant Functional Group Characteristics and Nitrogen and Phosphorus Stoichiometry in Alpine Semi-Humid Sandy Land. LID - 10.3390/plants15203083 [doi] LID - 3083 AB - Ecological restoration of alpine sandy lands plays an important role in promoting vegetation recovery and enhancing ecosystem functions. However, the response characteristics of plant functional groups and nitrogen (N) and phosphorus (P) dynamics under different restoration treatments remain unclear. Taking three typical ecological restoration treatments in the alpine semi-humid sandy land on the eastern edge of the Qinghai-Tibet Plateau-fencing + sand barrier establishment + mixed grass seeding (MG), fencing + sand barrier establishment + shrub planting alone (SA), and fencing + sand barrier establishment + shrub-grass combination (SG)-as the study objects, and unrestored natural sandy land as the control (CK), this study investigated the characteristics of plant biomass and diversity, as well as the content and stoichiometric characteristics of N and P in the plant-litter system under different restoration treatments, and analyzed the response relationships between plant diversity and changes in N and P in the plant-litter system. The results showed that all three restoration treatments increased plant community biomass and diversity indices. Among them, the SG treatment had the highest aboveground biomass and diversity, whereas the MG treatment had the largest belowground biomass. All three restoration treatments increased the N and P contents of herbaceous plants and litter, with the greatest increases observed under the SG treatment. The results for plant functional groups showed that legumes had the highest N content, while forbs had the highest P content. The N:P ratios of aboveground plant parts ranged from 5.85 to 8.82 and were lower in the SA and SG treatments, suggesting that plants under the restoration treatments may be N-limited. In conclusion, the SG treatment is the most effective restoration approach for promoting vegetation recovery and nutrient content in alpine sandy lands. These results provide a scientific basis for selecting ecological restoration treatments for alpine sandy lands and for improving restoration effectiveness and efficiency. FAU - Li, Yanling AU - Li Y AD - School of Management, Sichuan Agricultural University, Chengdu 611130, China. FAU - Yang, Nairui AU - Yang N AD - College of Resources, Sichuan Agricultural University, Chengdu 611130, China. FAU - Qian, Hongyu AU - Qian H AUID- ORCID: 0009-0002-0908-3757 AD - College of Resources, Sichuan Agricultural University, Chengdu 611130, China. FAU - Tong, Yuhong AU - Tong Y AD - College of Resources, Sichuan Agricultural University, Chengdu 611130, China. FAU - Song, Changjiang AU - Song C AD - College of Resources, Sichuan Agricultural University, Chengdu 611130, China. FAU - Gou, Ranxi AU - Gou R AD - College of Landscape Architecture, Sichuan Agricultural University, Chengdu 611130, China. FAU - Lin, Li AU - Lin L AD - School of Materials Science and Engineering, Sichuan University of Science and Engineering, Zigong 643000, China. FAU - Zhou, Li AU - Zhou L AD - Yibin Academy of Agricultural Sciences, Yibin 644600, China. LA - eng GR - 2022YFS0469/Sichuan Provincial Department of Science and Technology/ GR - S202510626001X/Sichuan Agricultural University/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13708296 OTO - NOTNLM OT - alpine sandy land OT - ecological restoration OT - plant diversity OT - plant functional groups OT - stoichiometric ratio COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:44 PHST- 2026/07/17 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - plants15203083 [pii] AID - plants-15-03083 [pii] AID - 10.3390/plants15203083 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 9;15(20):3083. doi: 10.3390/plants15203083. PMID- 42943035 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2223-7747 (Print) IS - 2223-7747 (Electronic) IS - 2223-7747 (Linking) VI - 15 IP - 20 DP - 2026 Oct 9 TI - Split Versus Single Applications of Solid and Liquid Vermicompost: Impacts on Growth, Chlorophyll Content and Soil Characteristics of Tea (Camellia sinensis L.) Seedlings. LID - 10.3390/plants15203082 [doi] LID - 3082 AB - Limited information is available on how vermicompost form, form-specific dose, and application schedule jointly affect tea seedling development. This 135-day humid outdoor nursery study evaluated solid and liquid vermicompost applied at two doses as either single or three split applications to 270 individually potted Camellia sinensis seedlings assigned to eight factorial treatments and an untreated control. Repeated measurements of seedling height, root collar diameter, and leaf chlorophyll content were analyzed using linear mixed-effects models fitted by restricted maximum likelihood (REML); the control was retained only in supplementary one-way ANOVAs. Soil pH, electrical conductivity (EC), and temperature were analyzed by two-way ANOVA. Vermicompost form, measurement time, and their interaction significantly affected seedling height and root collar diameter. Application schedule significantly affected root collar diameter (F = 7.35, p = 0.007) and chlorophyll content (F = 19.74, p < 0.001), but not seedling height, whereas dose within form showed no significant main effect on any of these traits. The form x application schedule x time interaction was significant for seedling height, whereas dose within form x application schedule x time was significant for root collar diameter and chlorophyll content. Treatment differences generally emerged from day 30 onward. The 30 g solid vermicompost treatment applied in three equal doses produced the highest final seedling height (24.80 cm) and root collar diameter (14.00 mm) and the highest chlorophyll content recorded during the experiment (914.98 micromol m(-2) on day 75). Treatment, measurement time, and their interaction significantly affected soil pH, EC, and temperature; pH remained within 4.90-5.76, and EC values did not indicate salinity stress. Selected split applications of solid vermicompost therefore showed potential to improve tea seedling growth, but significant higher-order interactions demonstrated that responses depended on vermicompost form, dose within form, application schedule, trait, and measurement time. FAU - Oguzturk, Turker AU - Oguzturk T AUID- ORCID: 0000-0002-9611-9959 AD - Department of Landscape Architecture, Faculty of Engineering and Architecture, Recep Tayyip Erdogan University, Rize 53020, Turkiye. FAU - Yuksek, Turan AU - Yuksek T AD - Department of Landscape Architecture, Faculty of Engineering and Architecture, Recep Tayyip Erdogan University, Rize 53020, Turkiye. FAU - Yuksek, Filiz AU - Yuksek F AUID- ORCID: 0000-0003-3275-642X AD - Rize Forest Enterprise Directorate, Trabzon Regional Directorate of Forestry, General Directorate of Forestry, Rize 53020, Turkiye. FAU - Sipahi, Merve AU - Sipahi M AUID- ORCID: 0000-0002-3246-6302 AD - Department of Interior Architecture and Environmental Design, Faculty of Architecture, Design and Fine Arts, Osmaniye Korkut Ata University, Osmaniye 80000, Turkiye. FAU - Pirim, Bahriye AU - Pirim B AD - Department of Landscape Architecture, Faculty of Engineering and Architecture, Recep Tayyip Erdogan University, Rize 53020, Turkiye. FAU - Erkmen, Rabia AU - Erkmen R AD - Department of Landscape Architecture, Faculty of Engineering and Architecture, Recep Tayyip Erdogan University, Rize 53020, Turkiye. FAU - Ercan Oguzturk, Gulcay AU - Ercan Oguzturk G AD - Department of Landscape Architecture, Faculty of Engineering and Architecture, Recep Tayyip Erdogan University, Rize 53020, Turkiye. LA - eng GR - Recep Tayyip Erdogan University/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Plants (Basel) JT - Plants (Basel, Switzerland) JID - 101596181 PMC - PMC13709122 OTO - NOTNLM OT - growth performance OT - soil properties OT - split application OT - tea seedlings OT - vermicompost COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:44 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - plants15203082 [pii] AID - plants-15-03082 [pii] AID - 10.3390/plants15203082 [doi] PST - epublish SO - Plants (Basel). 2026 Oct 9;15(20):3082. doi: 10.3390/plants15203082. PMID- 42943028 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 21 TI - Self-Related Cognitive Schemas, Negative Self-View, and Perceived Psychosocial Burden in Adults with Celiac Disease: A Cross-Sectional Comparative Study. LID - 10.3390/nu18203457 [doi] LID - 3457 AB - Background: Self-related cognitive schemas may influence psychological adaptation to chronic illness. However, their role in adults with celiac disease (CD) remains poorly understood. We aimed to assess cognitive schemas and depressive symptoms in adults with CD compared with healthy controls, and to examine their associations with perceived disease burden. Methods: This cross-sectional comparative study included 96 adults with CD and 96 healthy controls. Participants completed the Cognitive Triad Inventory (CTI) and Beck Depression Inventory (BDI), and sociodemographic and clinical data were collected. Perceived disease- and diet-related burden was assessed using investigator-developed single-item measures. Results: The mean age at CD diagnosis was 33.6 (SD 13.4) years, and the mean duration of symptoms before diagnosis was 5.4 (SD 5.1) years. Adults with CD reported higher depressive symptom scores than healthy controls (8.4 (SD 7.9) vs. 6.6 (SD 8.0); Mann-Whitney U = 3850.5, Z = 1.99, Cohen's d = 0.29, p = 0.046). No significant between-group differences were observed in total CTI scores or its Self-view, World-view, or Future-view subscales. After adjustment for age, sex, educational level, marital status, and employment status, neither BDI nor CTI scores were independently associated with group membership. Exploratory bivariate analyses within the CD group showed that higher Self-view scores were associated with lower perceived negative impact of gluten-free diet requirements on family life (rS = -0.29, p = 0.004) and lower perceived impact of CD symptoms and disease exacerbations on everyday life (rS = -0.31, p = 0.002). These associations remained statistically significant after Bonferroni correction. Conclusions: Adults with CD reported slightly higher depressive symptom levels than healthy controls, whereas cognitive schemas did not differ significantly between groups. The observed associations between CTI Self-view and selected indicators of perceived disease- and diet-related burden warrant further investigation, but do not establish a causal contribution of self-view to disease burden. FAU - Kot, Kinga AU - Kot K AD - Poznan Institute of Psychology and Pedagogy, Varsovia University of Business & Applied Sciences, 02-304 Warszawa, Poland. FAU - Sobieska-Poszwa, Ola AU - Sobieska-Poszwa O AD - Department of Pediatrics, Allergology and Gastroenterology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland. FAU - Dobrowolska, Agnieszka AU - Dobrowolska A AUID- ORCID: 0000-0002-3647-5070 AD - Poznan University of Medical Sciences, Poland, Department and Clinic of Gastroenterology, Dietetics and Internal Diseases, 60-512 Poznan, Poland. FAU - Krela-Kazmierczak, Iwona AU - Krela-Kazmierczak I AD - Poznan University of Medical Sciences, Poland, Department and Clinic of Gastroenterology, Dietetics and Internal Diseases, 60-512 Poznan, Poland. FAU - Misterska, Ewa AU - Misterska E AUID- ORCID: 0000-0001-9726-9214 AD - Department of Mental Health, Poznan University of Medical Sciences, 60-512 Poznan, Poland. LA - eng GR - Poznan University of Medical Sciences/ PT - Comparative Study PT - Journal Article DEP - 20261021 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 SB - IM MH - Humans MH - *Celiac Disease/psychology/diet therapy MH - Cross-Sectional Studies MH - Adult MH - Female MH - Male MH - Depression/psychology/etiology MH - *Self Concept MH - *Cognition MH - Middle Aged MH - *Cost of Illness MH - Adaptation, Psychological MH - Young Adult PMC - PMC13708285 OTO - NOTNLM OT - celiac disease OT - cognitive schemas OT - depression OT - gluten-free-diet COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/21 CRDT- 2026/10/27 10:44 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - nu18203457 [pii] AID - nutrients-18-03457 [pii] AID - 10.3390/nu18203457 [doi] PST - epublish SO - Nutrients. 2026 Oct 21;18(20):3457. doi: 10.3390/nu18203457. PMID- 42943020 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 21 TI - Emerging Therapeutic Strategies for Child and Adolescent Obesity: A Narrative Review. LID - 10.3390/nu18203449 [doi] LID - 3449 AB - BACKGROUND/OBJECTIVES: Childhood obesity is a chronic, multifactorial disease requiring attention to biological, behavioral, psychological, family, and socioeconomic factors. This narrative review examined the integration of lifestyle, family-based, psychological, and pharmacological approaches, focusing on treatment engagement, eating disorders, attention-deficit/hyperactivity disorder (ADHD), and treatment durability. METHODS: A single search interface covering PubMed/MEDLINE, Scopus, and Web of Science (the University of Turin library discovery platform) was used to identify studies published primarily from 2010 to August 2026. Pediatric trials, observational studies, systematic reviews, meta-analyses, guidelines, and public-health reports were prioritized. RESULTS: Intensive family-based, multicomponent behavioral treatment remains the best-supported foundation of care, although psychological outcomes are infrequently assessed. Psychiatric comorbidity is heterogeneous; loss-of-control eating, emotional symptoms, ADHD, and executive-function difficulties may impair engagement in selected patients, and untreated ADHD has been associated with a slower BMI response to weight management. Professionally supervised obesity treatment containing a dietary component did not, on average, worsen eating psychopathology and reduced several related symptoms. Family participation improved outcomes, whereas logistical, socioeconomic, and psychological barriers reduced attendance. Across randomized trials, adding anti-obesity medication to behavioral treatment produced an additional, although on average modest, reduction in BMI. Semaglutide, liraglutide, and phentermine/topiramate produced larger numerical reductions than older agents in separate trials, but heterogeneity and the absence of head-to-head comparisons preclude formal ranking. Gastrointestinal adverse events were frequent with glucagon-like peptide-1 (GLP-1) receptor agonists. Pediatric trials and real-world evidence indicate partial weight regain after discontinuation, while prescribing remains limited and unequal. CONCLUSIONS: Pediatric obesity care should be phenotype-informed, multidisciplinary, and longitudinal. Pharmacotherapy can enhance BMI reduction but should complement rather than replace behavioral, psychological, and family care. Outcomes should extend beyond BMI to metabolic, psychological, functional, and long-term measures. FAU - Oliaro, Tomaso AU - Oliaro T AD - Department of Public Health and Pediatric Sciences, University of Turin, 10126 Turin, Italy. FAU - Amianto, Federico AU - Amianto F AUID- ORCID: 0000-0002-7079-8391 AD - Neurosciences Department, University of Turin, 10126 Turin, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 53AXN4NNHX (Semaglutide) RN - 0 (Anti-Obesity Agents) SB - IM MH - Humans MH - Child MH - *Pediatric Obesity/therapy/psychology MH - Adolescent MH - Semaglutide MH - *Anti-Obesity Agents/therapeutic use MH - Behavior Therapy/methods MH - Attention Deficit Disorder with Hyperactivity MH - Feeding and Eating Disorders PMC - PMC13708364 OTO - NOTNLM OT - behavioral treatment OT - child obesity OT - drug treatment OT - family treatment OT - long-term effects OT - multidisciplinary approach COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/21 CRDT- 2026/10/27 10:44 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - nu18203449 [pii] AID - nutrients-18-03449 [pii] AID - 10.3390/nu18203449 [doi] PST - epublish SO - Nutrients. 2026 Oct 21;18(20):3449. doi: 10.3390/nu18203449. PMID- 42943007 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 20 TI - Ketones and Sleep: A Narrative Review of Mechanisms, Preclinical Models, and Human Evidence. LID - 10.3390/nu18203437 [doi] LID - 3437 AB - BACKGROUND/OBJECTIVES: Sleep is a metabolically dynamic state, and ketone bodies may interact with sleep physiology as both oxidative substrates and signaling metabolites. beta-Hydroxybutyrate (BHB), acetoacetate (AcAc), and acetone rise during fasting, carbohydrate restriction, ketogenic diets used for therapeutic or non-therapeutic purposes, prolonged exercise, selected pathological states of insulin deficiency, and after consumption of exogenous ketones. This narrative review synthesizes mechanistic, preclinical, and human evidence linking endogenously generated ketosis and ketosis generated from exogenous ketone consumption to sleep architecture, sleep quality, sleep-disordered breathing, and sleep-relevant metabolic pathways. METHODS: We reviewed studies involving ketogenic diets, fasting or caloric restriction, and consumption of exogenous ketones, prioritizing investigations with polysomnography, electroencephalography (EEG), sleep architecture, respiratory, or validated sleep-questionnaire outcomes, while also considering mechanistic studies of neuronal excitability, inflammation, epigenetic regulation, circadian timing, and brain energetics. RESULTS: The most direct preclinical evidence indicates that AcAc, but not BHB, increases slow-wave activity (delta power 0.5-4 Hz) during non-rapid eye movement (NREM) sleep and suppresses glutamate release in mice, supporting a potential role for ketone metabolism in sleep homeostasis. Dietary, fasting, and aging models further suggest that lipid/ketone metabolism can alter NREM/rapid eye movement (REM) balance, circadian metabolic rhythms, and sleep-wake responses to energetic stress, with aged-rat data showing sex-specific REM and cognitive effects of ketogenic interventions. Human studies are suggestive but heterogeneous: acute carbohydrate restriction can increase slow-wave sleep and reduce REM sleep in healthy adults; ketogenic therapies may improve subjective sleep in selected neurological and metabolic populations; and ketone ester ingestion can improve sleep efficiency after strenuous evening exercise, but not during hypoxia-induced sleep disruption. CONCLUSIONS: Ketones are sleep-relevant metabolic signals, but their effects are conditional rather than universally sleep-promoting. They may also indirectly affect sleep by enhancing wakefulness quality. The ketone concentration range associated with optimal sleep outcomes remains undefined, and potential dose-, timing-, and context-dependent effects on sleep require further study. Future studies should distinguish AcAc from BHB biology, measure nocturnal ketone dynamics, incorporate PSG/EEG spectral endpoints, and separate ketosis-specific effects from weight loss, energy deficit, exercise load, respiratory physiology, circadian timing, and disease-related confounders. FAU - Jensen, Jack C AU - Jensen JC AD - Mass General Brigham Department of Anesthesiology, Massachusetts General Hospital, Charlestown, MA 02129, USA. FAU - Leavitt, Parker C AU - Leavitt PC AD - Mass General Brigham Department of Anesthesiology, Massachusetts General Hospital, Charlestown, MA 02129, USA. FAU - Nehs, Christa J AU - Nehs CJ AUID- ORCID: 0000-0002-6708-1656 AD - Mass General Brigham Department of Anesthesiology, Massachusetts General Hospital, Charlestown, MA 02129, USA. AD - Division of Sleep Medicine, Harvard Medical School, Boston, MA 02155, USA. AD - Metabolic and Mental Health Program, McLean Hospital, Belmont, MA 02478, USA. LA - eng GR - R01 AG076704/AG/NIA NIH HHS/United States GR - R01AG076704 to CJN/AG/NIA NIH HHS/United States PT - Journal Article PT - Review DEP - 20261020 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Ketones) RN - 0 (Ketone Bodies) RN - 0 (Acetoacetates) RN - 4ZI204Y1MC (acetoacetic acid) SB - IM MH - Humans MH - *Sleep/physiology MH - Animals MH - Diet, Ketogenic MH - Ketosis/metabolism MH - *Ketones/metabolism MH - Ketone Bodies/metabolism MH - Acetoacetates/metabolism MH - Caloric Restriction MH - Fasting PMC - PMC13708312 OTO - NOTNLM OT - REM sleep OT - acetoacetate OT - beta-hydroxybutyrate OT - circadian rhythm OT - exogenous ketones OT - inflammation OT - ketogenic diet OT - obstructive sleep apnea OT - sleep architecture OT - slow-wave sleep COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/20 CRDT- 2026/10/27 10:44 PHST- 2026/07/31 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - nu18203437 [pii] AID - nutrients-18-03437 [pii] AID - 10.3390/nu18203437 [doi] PST - epublish SO - Nutrients. 2026 Oct 20;18(20):3437. doi: 10.3390/nu18203437. PMID- 42943005 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 20 TI - Effects of Caffeine-Taurine Co-Supplementation on Exercise Performance: A Systematic Review and Meta-Analysis. LID - 10.3390/nu18203434 [doi] LID - 3434 AB - Background: Caffeine-taurine combinations are widely used in commercial sports products. Whether this combination improves exercise performance is still unclear. This study aimed to estimate the effect of caffeine-taurine co-supplementation versus placebo on exercise performance. Methods: Randomized controlled trials comparing caffeine plus taurine versus placebo were searched in PubMed, Embase, Web of Science, Cochrane Library, and related databases up to June 1, 2026. Outcomes included heart rate (HR), reaction time (RT), time to exhaustion (TTE), rating of perceived exertion (RPE), and peak/maximal anaerobic power (PP). A random-effects model was applied. Risk of bias was assessed with RoB 2.0. Results: A total of 11 randomized controlled trials involving 234 participants were included in the meta-analysis. Compared with placebo, caffeine-taurine co-supplementation was associated with improvements in time to exhaustion (SMD = 0.54, 95% CI: 0.03 to 1.05) and PP (SMD = 0.55, 95% CI: 0.21 to 0.89); an expanded secondary product-level analysis that additionally included one commercial multi-ingredient energy drink yielded a smaller estimate (SMD = 0.48, 95% CI: 0.19 to 0.78) that cannot be attributed specifically to caffeine and taurine. The pooled estimate for reaction time favored supplementation (SMD = -1.49, 95% CI: -2.78 to -0.20), but very high heterogeneity and a prediction interval crossing the null indicated that the effect was not consistent across settings, whereas no meaningful effect was observed for heart rate (WMD = -0.11, 95% CI: -5.76 to 5.53). Rating of perceived exertion was significantly reduced (SMD = -0.44, 95% CI: -0.83 to -0.04). Conclusions: Caffeine-taurine-only formulations were associated with possible benefits for selected performance outcomes, including PP. However, the evidence was derived from few, generally small studies and did not establish the independent contribution of caffeine, taurine, or their interaction. Results from commercial multi-ingredient products should be interpreted at the product level rather than as evidence of an isolated caffeine-taurine effect. FAU - He, Bingbing AU - He B AD - School of Physical Education and Sports, Beijing Normal University, Beijing 100875, China. FAU - Zhou, Chengyu AU - Zhou C AD - School of Strength and Conditioning Training, Beijing Sport University, Beijing 100084, China. FAU - Zeng, Zhuo AU - Zeng Z AUID- ORCID: 0009-0001-7689-6873 AD - School of Strength and Conditioning Training, Beijing Sport University, Beijing 100084, China. FAU - Dong, Xusong AU - Dong X AD - School of Strength and Conditioning Training, Beijing Sport University, Beijing 100084, China. FAU - Huang, Dongxu AU - Huang D AD - School of Strength and Conditioning Training, Beijing Sport University, Beijing 100084, China. FAU - Shi, Haoqiang AU - Shi H AD - School of Strength and Conditioning Training, Beijing Sport University, Beijing 100084, China. FAU - Li, Haoran AU - Li H AD - School of Strength and Conditioning Training, Beijing Sport University, Beijing 100084, China. FAU - Gao, Kai AU - Gao K AD - College of Sports and Health, Linyi University, Linyi 276005, China. AD - Department of Sports Science, The University of Suwon, Hwaseong-si 18323, Republic of Korea. FAU - Xie, Yongmin AU - Xie Y AD - School of Strength and Conditioning Training, Beijing Sport University, Beijing 100084, China. FAU - Zhou, Aiguo AU - Zhou A AD - School of Strength and Conditioning Training, Beijing Sport University, Beijing 100084, China. FAU - Lang, Diandong AU - Lang D AD - College of Sports and Health, Linyi University, Linyi 276005, China. FAU - Zhang, Qinshuo AU - Zhang Q AD - School of Physical Education and Sports, Beijing Normal University, Beijing 100875, China. LA - eng PT - Journal Article PT - Meta-Analysis PT - Review PT - Systematic Review DEP - 20261020 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 3G6A5W338E (Caffeine) RN - 1EQV5MLY3D (Taurine) SB - IM MH - *Caffeine/administration & dosage/pharmacology MH - *Taurine/administration & dosage/pharmacology MH - Humans MH - *Dietary Supplements MH - *Athletic Performance/physiology MH - Randomized Controlled Trials as Topic MH - Heart Rate/drug effects MH - Reaction Time/drug effects PMC - PMC13708174 OTO - NOTNLM OT - caffeine OT - exercise performance OT - meta-analysis OT - systematic review OT - taurine COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/20 CRDT- 2026/10/27 10:44 PHST- 2026/07/13 00:00 [received] PHST- 2026/08/14 00:00 [revised] PHST- 2026/08/18 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - nu18203434 [pii] AID - nutrients-18-03434 [pii] AID - 10.3390/nu18203434 [doi] PST - epublish SO - Nutrients. 2026 Oct 20;18(20):3434. doi: 10.3390/nu18203434. PMID- 42943002 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 19 TI - Melatonin and Female Fertility: A Systematic Review and Meta-Analysis of Clinical Supplementation, In Vitro Experiments, and Observational Evidence. LID - 10.3390/nu18203431 [doi] LID - 3431 AB - Background/Objectives: Melatonin has been proposed as a potential strategy to improve female fertility, but existing evidence is dispersed across assisted reproduction, reproductive disorders, and in vitro studies. This study aimed to comprehensively evaluate the relationships of melatonin supplementation, in vitro melatonin treatment, and endogenous melatonin levels with female fertility. Methods: PubMed, Web of Science, and EMBASE were searched from inception through February 2026. Randomized and non-randomized intervention studies, observational studies, and in vitro studies involving human oocytes or embryos were included. Random-effects meta-analyses were performed, and certainty of evidence was assessed using GRADE. Results: Thirty-six publications were included. Among women undergoing ART, melatonin supplementation increased the clinical pregnancy rate (RR = 1.55, 95% CI: 1.23-1.94) and live birth (RR = 1.59, 95% CI: 1.01-2.51). Melatonin supplementation also improved selected ovarian and embryological outcomes. In vitro treatment increased fertilization (OR = 1.97, 95% CI: 1.40-2.76) and blastocyst formation (OR = 2.00, 95% CI: 1.33-3.03). Observational studies showed higher morning serum melatonin levels in women with PCOS (MD = 7.83 pg/mL, 95% CI: 3.56-12.11). The certainty of evidence ranged from very low to moderate. Conclusions: Melatonin supplementation may improve selected reproductive outcomes, including clinical pregnancy and live birth, although evidence for live birth remains limited. In vitro and observational findings further support a potential role of melatonin in female reproductive function, but its clinical value requires confirmation in high-quality studies. FAU - Ye, Hanqi AU - Ye H AD - Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing 210009, China. FAU - Hu, Yijia AU - Hu Y AD - Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing 210009, China. FAU - Wang, Han AU - Wang H AD - Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing 210009, China. FAU - Shi, Yumo AU - Shi Y AD - Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing 210009, China. FAU - Wang, Bei AU - Wang B AD - Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing 210009, China. FAU - Yin, Jiechen AU - Yin J AD - Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing 210009, China. FAU - Hong, Xiang AU - Hong X AD - Department of Epidemiology and Health Statistics, School of Public Health, Southeast University, Nanjing 210009, China. LA - eng GR - 82404284; 82574109/National Natural Science Foundation of China/ PT - Journal Article PT - Meta-Analysis PT - Review PT - Systematic Review DEP - 20261019 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - JL5DK93RCL (Melatonin) SB - IM MH - Humans MH - Female MH - *Melatonin/pharmacology/administration & dosage/blood MH - Pregnancy MH - *Dietary Supplements MH - *Fertility/drug effects MH - Pregnancy Rate MH - Observational Studies as Topic MH - *Infertility, Female/drug therapy MH - Adult MH - Fertilization in Vitro MH - Live Birth PMC - PMC13708123 OTO - NOTNLM OT - assisted reproductive technology OT - dietary supplement OT - embryo development OT - female fertility OT - melatonin OT - oocytes OT - polycystic ovary syndrome COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/19 CRDT- 2026/10/27 10:44 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - nu18203431 [pii] AID - nutrients-18-03431 [pii] AID - 10.3390/nu18203431 [doi] PST - epublish SO - Nutrients. 2026 Oct 19;18(20):3431. doi: 10.3390/nu18203431. PMID- 42942994 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 19 TI - Joint Modelling Application to Identify Key Determinants of Malnutrition and Anemia in Five West African Countries. LID - 10.3390/nu18203423 [doi] LID - 3423 AB - OBJECTIVES: In Sub-Saharan Africa, anemia significantly contributes to infant mortality and morbidity, particularly among children affected by malnutrition. Given the intertwined nature of these conditions, separate modeling approaches are insufficient for capturing their combined effects. Joint modeling is essential to concurrently analyze anemia and malnutrition, enabling more accurate parameter estimation and a comprehensive understanding of shared risk factors. This study investigates determinants of both conditions across select West African countries, including Gabon, Gambia, Liberia, Mauritania, and Nigeria, with the aim of guiding policy measures to reduce prevalence. A novel aspect of this research is the joint modeling framework developed to analyze anemia and malnutrition together, an approach that remains limited in existing literature. By incorporating data from multiple countries, this study provides a unique and regionally contextualized perspective, addressing critical gaps in policy-relevant research. DESIGN: A multivariate joint model was used to identify significant predictors of anemia and malnutrition in children under five years of age. DATA SOURCE: This secondary data analysis utilized demographic and health survey (DHS) data collected in selected West African countries. The DHS applied a complex, multistage sampling design incorporating stratification and cluster sampling, ensuring that the resulting weighted samples accurately represented various population subgroups. RESULTS: The model results identified several statistically significant determinants (p < 0.05) of malnutrition and anemia. These factors included the mother's age, the highest level of maternal education, source of drinking water, type of toilet facility, household electricity access, type of cooking fuel used, household wealth index, whether the child was given fortified food, the child's age and gender and whether the child had a fever in the past two weeks. CONCLUSIONS: This study highlights the essential role of primary healthcare interventions, community outreach, and policy initiatives in mitigating malnutrition and anemia in West African nations. Key measures include routine anemia screening for high-risk mothers, implementing standardized treatment protocols, and addressing environmental risk factors. Partnerships with local governments and nonprofit organizations are vital for providing vulnerable groups with access to fortified foods, nutritional support, and essential healthcare services. Additionally, mobile health tools and educational webinars can address literacy barriers and enhance antenatal care. A comprehensive approach incorporating education, healthcare, and technology is crucial for improving health outcomes and reducing the prevalence of these conditions. FAU - Beni, Reshav AU - Beni R AUID- ORCID: 0000-0001-8657-4344 AD - School of Mathematics, Statistics and Computer Science, University of KwaZulu-Natal, Pietermaritzburg 3209, South Africa. FAU - Ramroop, Shaun AU - Ramroop S AUID- ORCID: 0000-0001-9305-6967 AD - School of Mathematics, Statistics and Computer Science, University of KwaZulu-Natal, Pietermaritzburg 3209, South Africa. FAU - Habyarimana, Faustin AU - Habyarimana F AUID- ORCID: 0000-0003-0240-5186 AD - School of Mathematics, Statistics and Computer Science, University of KwaZulu-Natal, Pietermaritzburg 3209, South Africa. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 SB - IM MH - Humans MH - *Anemia/epidemiology/etiology MH - Female MH - Africa, Western/epidemiology MH - Infant MH - Child, Preschool MH - Risk Factors MH - *Malnutrition/epidemiology MH - Male MH - Prevalence MH - Secondary Data Analysis MH - West African People MH - Adult MH - Health Surveys PMC - PMC13708424 OTO - NOTNLM OT - Western Africa OT - anemia OT - joint model OT - malnutrition OT - public health COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/19 CRDT- 2026/10/27 10:44 PHST- 2026/09/19 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - nu18203423 [pii] AID - nutrients-18-03423 [pii] AID - 10.3390/nu18203423 [doi] PST - epublish SO - Nutrients. 2026 Oct 19;18(20):3423. doi: 10.3390/nu18203423. PMID- 42942988 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 18 TI - Human Milk Oligosaccharides in Exercise Nutrition: Structure-Specific Evidence, Human Efficacy, and Translational Bottlenecks. LID - 10.3390/nu18203417 [doi] LID - 3417 AB - Background/Objectives: Human milk oligosaccharides (HMOs) are structurally diverse bioactive carbohydrates being investigated beyond early-life nutrition. General gut-muscle and short-chain fatty acid (SCFA) frameworks are well described. This review aimed to assess structure-specific evidence for HMO effects on human exercise outcomes and identify the main translational bottlenecks. Methods: This critical translational review systematically searched PubMed/MEDLINE (Medical Literature Analysis and Retrieval System Online), Embase, Web of Science Core Collection, Scopus, and SPORTDiscus. Evidence was assigned non-exclusively to Layer A (direct exercise evidence), Layer B (exercise-relevant mechanisms), or Layer C (adult translational evidence). We separately evaluated HMO structure, prespecified human efficacy outcomes, within-model causal support, and distance from healthy human exercise. Results: The five databases yielded 4787 records; 40 reports representing 38 independent studies were included. Several HMOs were tolerated at the doses and durations tested and produced structure-, dose-, and host-dependent microbial responses; limited systemic exposure was documented for selected structures. Some intestinal and disease-model pathways had perturbation, knockout, transfer, or rescue support. However, direct human exercise evidence was sparse and inconsistent: primary or overall interaction tests were generally null, whereas favorable findings were mainly secondary, interim, within-group, or exploratory. No study established the complete sequence from oral exposure to target engagement, healthy skeletal-muscle adaptation, and improved performance. Conclusions: Current evidence supports biological activity and testability of selected HMOs, but not an HMO class effect, a confirmed ergogenic mechanism, or a recommendation for exercise-performance use. Translation is constrained by four unresolved links: structure to exposure, exposure to target engagement, target engagement to muscle phenotype, and muscle phenotype to functional benefit. FAU - Qiao, Chuyuan AU - Qiao C AUID- ORCID: 0009-0006-7735-3420 AD - School of Sports Medicine and Rehabilitation, Beijing Sport University, Beijing 100084, China. FAU - Guo, Sitong AU - Guo S AUID- ORCID: 0009-0009-5008-8454 AD - National Institute of Biological Sciences, Beijing 102206, China. FAU - Yang, Shenglei AU - Yang S AUID- ORCID: 0009-0002-3236-5218 AD - School of Sports Medicine and Rehabilitation, Beijing Sport University, Beijing 100084, China. FAU - Yang, Chuwei AU - Yang C AD - School of Sports Medicine and Rehabilitation, Beijing Sport University, Beijing 100084, China. FAU - Wang, Yan AU - Wang Y AD - School of Sports Medicine and Rehabilitation, Beijing Sport University, Beijing 100084, China. LA - eng PT - Journal Article PT - Review PT - Systematic Review DEP - 20261018 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Oligosaccharides) SB - IM MH - Humans MH - *Oligosaccharides/chemistry MH - *Milk, Human/chemistry MH - *Exercise/physiology MH - Female MH - Translational Research, Biomedical PMC - PMC13708468 OTO - NOTNLM OT - exercise nutrition OT - gut microbiota OT - human milk oligosaccharides OT - mitochondrial metabolism OT - skeletal muscle OT - training adaptation OT - translational evidence COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/18 CRDT- 2026/10/27 10:44 PHST- 2026/09/20 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - nu18203417 [pii] AID - nutrients-18-03417 [pii] AID - 10.3390/nu18203417 [doi] PST - epublish SO - Nutrients. 2026 Oct 18;18(20):3417. doi: 10.3390/nu18203417. PMID- 42942981 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 17 TI - Artificial Intelligence-Driven Dietary Assessment Software: A Structured Narrative Review of Technological Advances, Persistent Measurement Error and Barriers to Clinical Translation. LID - 10.3390/nu18203406 [doi] LID - 3406 AB - Background: Accurate dietary-intake assessment underpins nutrition research and clinical dietetics, yet self-report methods remain vulnerable to recall, portion-size and reporting error. Objective: This structured narrative review evaluates AI-enabled and sensor-supported dietary-assessment software, focusing on technical performance, measurement validity, user engagement, food-composition databases, cultural applicability and clinical readiness. Methods: Evidence was identified through searches of five electronic sources-PubMed (including MEDLINE-indexed records), Scopus, Web of Science Core Collection, IEEE Xplore and ScienceDirect-covering January 2020 to March 2025, and a complete rerun of the same five sources on 7 August 2026, supplemented by backward and forward citation tracking. The retained primary screening record comprised 1620 electronic-source records and 42 additional records (1662 before deduplication); 478 duplicates were removed, 1184 records were screened, 212 full texts were assessed and 15 sources were retained for the primary-phase qualitative synthesis. These figures are retained aggregate review-log totals and should not be interpreted as a fully reconstructable systematic-review audit trail. The 2026 update incorporated 18 publications: 10 from the January 2020-March 2025 historical interval through separate recovery/reassessment, and eight published after March 2025 through the 497-record update-specific screening stream, with retrieval phase reported separately from evidence role. The higher, moderate and limited/emerging within-map convergence descriptors summarise patterns in the purposively selected literature and do not represent exhaustive retrieval, formal certainty of evidence or reproducible screening. Results: Food recognition is most mature for visually distinct foods under controlled conditions. Portion-size estimation, nutrient quantification, mixed dishes, sustained engagement and culturally diverse foods remain less consistent. Selected systems show reasonable group-level energy and macronutrient agreement under defined conditions, whereas validation in free-living and clinical settings remains variable; a 2026 doubly labelled water study reported substantial underestimation and poor individual agreement in females with obesity. Conclusions: Current tools may support self-monitoring, research screening and dietitian-assisted assessment, but stand-alone use for high-stakes therapeutic decisions requires independent, nutrient-specific validation in the target population. FAU - Khan, Mohammad Idreesh AU - Khan MI AUID- ORCID: 0000-0001-9325-6491 AD - Department of Basic Health Sciences, College of Applied Medical Sciences, Qassim University, P.O. Box 6666, Buraydah 51452, Saudi Arabia. FAU - Turki, Abrar AU - Turki A AUID- ORCID: 0000-0002-3956-1150 AD - Clinical Nutrition Department, College of Applied Medical Sciences, University of Hafr Al Batin, Hafr Al Batin 39524, Saudi Arabia. FAU - Alanezi, Shadaid AU - Alanezi S AUID- ORCID: 0009-0002-6667-5150 AD - Department of Health Information Management and Technology, College of Applied Medical Sciences, University of Hafr Al Batin, Hafr Al Batin 39524, Saudi Arabia. FAU - Almutairi, Malak Ghazi AU - Almutairi MG AUID- ORCID: 0000-0001-7759-6437 AD - Clinical Nutrition Department, College of Applied Medical Sciences, University of Hafr Al Batin, Hafr Al Batin 39524, Saudi Arabia. FAU - Ashfaq, Fauzia AU - Ashfaq F AUID- ORCID: 0000-0003-3653-4381 AD - Department of Clinical Nutrition, College of Nursing and Health Sciences, Jazan University, Jazan 45142, Saudi Arabia. FAU - Khan, Mohd Faheem AU - Khan MF AUID- ORCID: 0000-0002-8911-3268 AD - Department of Biotechnology and Microbiology, Khandelwal College of Management, Science and Technology, M.J.P. Rohilkhand University, Bareilly 243006, India. LA - eng GR - Acknowledgments: The Researchers would like to thank the Deanship of Graduate Studies and Scientific Research at Qassim University (www.qu.edu.sa) for financial support (QU-APC-2026)./Qassim University/ PT - Journal Article PT - Review DEP - 20261017 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 SB - IM MH - Humans MH - *Artificial Intelligence MH - *Nutrition Assessment MH - *Software MH - Dietetics/methods MH - Translational Research, Biomedical MH - Reproducibility of Results MH - Diet Records PMC - PMC13708203 OTO - NOTNLM OT - artificial intelligence OT - computer vision OT - dietary assessment OT - mobile health OT - nutrition software OT - precision nutrition OT - wearable sensors COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/17 CRDT- 2026/10/27 10:44 PHST- 2026/08/01 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - nu18203406 [pii] AID - nutrients-18-03406 [pii] AID - 10.3390/nu18203406 [doi] PST - epublish SO - Nutrients. 2026 Oct 17;18(20):3406. doi: 10.3390/nu18203406. PMID- 42942968 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 16 TI - Short-Term Changes in Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD) and Related Circulating Biomarkers Following a Very-Low Calorie Ketogenic Diet (VLCKD): A Paired Longitudinal Analysis. LID - 10.3390/nu18203397 [doi] LID - 3397 AB - BACKGROUND: Metabolic dysfunction-associated steatotic liver disease (MASLD) is the most common chronic liver disease worldwide. Its prevalence is constantly increasing, particularly among obese individuals. Lifestyle modifications remain the first-line intervention for the management and treatment of MASLD. The most widely recommended dietary regime for MASLD is the Mediterranean Diet (MedDiet). Despite its countless benefits, this dietary regimen is difficult to follow in some cases. This study aimed to assess the effects of an 8-week Very Low-Calorie Ketogenic Diet (VLCKD) in obese patients with MASLD. METHODS: The study included 55 obese adults aged 18-64 years, recruited from the Center of Nutrition for Obesity and Metabolic Diseases, National Institute of Gastroenterology "Saverio de Bellis" Research Hospital (Castellana Grotte, Bari, Italy). Anthropometry, body composition (BIA), liver status (FibroScan((R))), serum biochemistry and circulating biomarkers of adipose dysfunction were assessed at baseline (T0) and post-intervention (T1). RESULTS: Significant improvements in liver steatosis (CAP p < 0.0001), liver stiffness (p = 0.0005), anthropometric parameters (waist p < 0.0001), and body composition (FM p <0.0001) were observed. A significant reduction in leptin levels and an increase in sex hormone-binding globulin (SHBG p < 0.0001) were also observed. CONCLUSIONS: In this uncontrolled pre-post analysis, the 8-week VLCKD intervention was associated with improvements in hepatic steatosis, body weight, and fat mass, and selected circulating biomarkers of adipose tissue dysfunction in adults with obesity and MASLD. FAU - Tatoli, Rossella AU - Tatoli R AUID- ORCID: 0000-0002-7891-6057 AD - Data Science Unit, National Institute of Gastroenterology IRCCS "Saverio de Bellis", 70013 Bari, Italy. FAU - Donghia, Rossella AU - Donghia R AUID- ORCID: 0000-0002-9140-673X AD - Data Science Unit, National Institute of Gastroenterology IRCCS "Saverio de Bellis", 70013 Bari, Italy. FAU - Pesole, Pasqua Letizia AU - Pesole PL AUID- ORCID: 0000-0003-4675-2075 AD - Core Facility Biobank, National Institute of Gastroenterology IRCCS "Saverio de Bellis", 70013 Bari, Italy. FAU - Di Chito, Martina AU - Di Chito M AUID- ORCID: 0009-0007-2759-8347 AD - Center of Nutrition for the Research and the Care of Obesity and Metabolic Diseases, National Institute of Gastroenterology IRCCS "Saverio de Bellis", 70013 Bari, Italy. FAU - Stabile, Dolores AU - Stabile D AD - Core Facility Biobank, National Institute of Gastroenterology IRCCS "Saverio de Bellis", 70013 Bari, Italy. FAU - Ferro, Annalisa AU - Ferro A AD - Laboratory of Clinical Pathology, National Institute of Gastroenterology IRCCS "Saverio de Bellis", 70013 Bari, Italy. FAU - Ancona, Anna AU - Ancona A AUID- ORCID: 0009-0009-7662-1763 AD - Core Facility Biobank, National Institute of Gastroenterology IRCCS "Saverio de Bellis", 70013 Bari, Italy. FAU - Casimo, Gianluigi AU - Casimo G AUID- ORCID: 0009-0000-2228-986X AD - Data Science Unit, National Institute of Gastroenterology IRCCS "Saverio de Bellis", 70013 Bari, Italy. FAU - Giannelli, Gianluigi AU - Giannelli G AUID- ORCID: 0000-0002-5140-8060 AD - Scientific Direction, National Institute of Gastroenterology IRCCS "Saverio de Bellis", 70013 Bari, Italy. LA - eng GR - Ricerca Corrente 2025-2026/Ministero della Salute/ GR - G23C22003360001/INNOVA-Hub Life Science-Advanced Diagnostics (HLS-DA)/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Biomarkers) SB - IM MH - Humans MH - Adult MH - Biomarkers/blood MH - Female MH - Male MH - Middle Aged MH - *Diet, Ketogenic/methods MH - *Obesity/diet therapy/complications/blood MH - Longitudinal Studies MH - Young Adult MH - *Caloric Restriction MH - *Fatty Liver/diet therapy/blood MH - Adolescent MH - Body Composition MH - Treatment Outcome PMC - PMC13708398 OTO - NOTNLM OT - MASLD OT - VLCKD OT - adipose dysfunction OT - body composition OT - liver steatosis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/16 CRDT- 2026/10/27 10:44 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:44 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - nu18203397 [pii] AID - nutrients-18-03397 [pii] AID - 10.3390/nu18203397 [doi] PST - epublish SO - Nutrients. 2026 Oct 16;18(20):3397. doi: 10.3390/nu18203397. PMID- 42942963 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 16 TI - The Preterm Brain Is Also What It Eats: A Systematic Review of Nutrition and Neuroimaging Outcomes in Preterm Infants. LID - 10.3390/nu18203392 [doi] LID - 3392 AB - Background: Nutrition is one of the most modifiable determinants of brain development in preterm infants. Although increasing evidence supports associations between early nutritional exposures and neurodevelopment, studies remain heterogeneous regarding nutritional interventions, imaging biomarkers, and long-term outcomes. This systematic review aimed to summarize contemporary evidence on the relationship between nutrition and brain development in preterm infants using neuroimaging as the primary assessment tool while proposing an integrated translational framework to guide future research. Methods: Following a PECO framework, a systematic literature search was conducted in PubMed, Scopus, and CINAHL: population, infants born before 37 completed weeks of gestation; exposure, any enteral or parenteral nutritional exposure, supplementation strategy, fortification approach, or individualized nutritional intervention; comparator, an alternative nutritional strategy, lower or absent exposure, standard care or, when relevant, a term-born reference group; outcomes, MRI- or CUS-based measures of brain growth, maturation, connectivity, or injury. Studies reporting volumetric, morphometric, diffusion, connectivity, spectroscopy, or quantitative ultrasound outcomes were eligible. The methodology was adapted from the PROSPERO-registered review by Ottolini et al. Original studies published between 2020 and 2026 (1 January 2020-4 September 2026) evaluating nutritional exposures or interventions in preterm infants with magnetic resonance imaging (MRI) and/or cranial ultrasound (CUS) outcomes were included. Risk of bias was assessed using the Newcastle-Ottawa Scale (NOS), ROBINS-I and RoB 2 according to study design. Quantitative imaging studies were screened for eligibility for ATLAS assessment. Given the substantial heterogeneity in nutritional exposures, imaging protocols, timing of assessments, and neurodevelopmental outcomes, meta-analysis was not performed. Certainty of evidence was assessed at the level of each causal question and neuroimaging outcome domain using the GRADE framework. Randomized intervention evidence and observational exposure evidence were evaluated according to the design and target contrast contributing to each body of evidence. Judgments considered risk of bias, inconsistency, indirectness, imprecision, publication bias, and, where applicable, factors that could increase certainty. Statistical significance was not used to determine certainty, and studies arising from shared or partially overlapping parent populations were not counted as independent replication. Results: We found 33 reports that met the inclusion criteria: 4 evaluated randomized treatment contrasts, 5 non-randomized intervention comparisons, and 24 observational nutritional exposure analyses. Greater exposure to human milk and selected strategies involving early protein and lipid provision, docosahexaenoic acid, omega-3 fatty acids, and individualized nutritional support were associated in several studies with favorable MRI measurements, including larger total or regional brain volumes, more advanced white-matter maturation, and differences in structural or functional connectivity. Some studies also reported associations with later neurodevelopment. However, findings were not uniform, and GRADE certainty across outcome-specific nutrition-neuroimaging questions ranged from very low to low, principally because of risk of bias, imprecision, heterogeneous exposure and imaging definitions, and limited independent replication. Although causal inference cannot be directly drawn, neuroimaging may provide quantitative measures of brain development that can be investigated alongside long-term neurodevelopmental outcomes. We propose the Roadmap for Imaging-Guided Enhancement of Neurodevelopment (RIGEL), an integrative research framework combining imaging, molecular biomarkers, standardized neurodevelopmental assessment, and epidemiological evidence across developmental stages. RIGEL should be regarded as a hypothesis-generating research framework that requires prospective validation before any clinical implementation. FAU - Canepa, Maria Ester AU - Canepa ME AUID- ORCID: 0009-0004-7400-1724 AD - Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics and Mother-Child Health, University of Genoa, 16132 Genoa, Italy. FAU - Prefumo, Federico AU - Prefumo F AUID- ORCID: 0000-0001-7793-714X AD - Obstetrics and Gynecology Unit, IRCCS Istituto Giannina Gaslini, 16147 Genoa, Italy. FAU - Biagioli, Valentina AU - Biagioli V AUID- ORCID: 0009-0004-3021-6627 AD - Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics and Mother-Child Health, University of Genoa, 16132 Genoa, Italy. FAU - Striano, Pasquale AU - Striano P AUID- ORCID: 0000-0002-6065-1476 AD - Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics and Mother-Child Health, University of Genoa, 16132 Genoa, Italy. AD - Pediatric Neurology and Muscular Diseases Unit, IRCCS Istituto Giannina Gaslini, 16147 Genoa, Italy. FAU - Calandrino, Andrea AU - Calandrino A AUID- ORCID: 0000-0002-2710-1798 AD - Neonatal Intensive Care Unit, IRCCS Istituto Giannina Gaslini, 16147 Genoa, Italy. FAU - Ramenghi, Luca Antonio AU - Ramenghi LA AD - Department of Neuroscience, Rehabilitation, Ophthalmology, Genetics and Mother-Child Health, University of Genoa, 16132 Genoa, Italy. AD - Neonatal Intensive Care Unit, IRCCS Istituto Giannina Gaslini, 16147 Genoa, Italy. LA - eng PT - Journal Article PT - Review PT - Systematic Review DEP - 20261016 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 SB - IM MH - Female MH - Humans MH - Infant, Newborn MH - *Brain/growth & development/diagnostic imaging MH - *Infant Nutritional Physiological Phenomena MH - *Infant, Premature/growth & development MH - Magnetic Resonance Imaging MH - *Neurodevelopment MH - *Neuroimaging MH - *Nutritional Status PMC - PMC13708710 OTO - NOTNLM OT - MRI OT - brain OT - development OT - growth OT - imaging OT - neonate OT - nutrition COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/16 CRDT- 2026/10/27 10:43 PHST- 2026/08/06 00:00 [received] PHST- 2026/09/12 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - nu18203392 [pii] AID - nutrients-18-03392 [pii] AID - 10.3390/nu18203392 [doi] PST - epublish SO - Nutrients. 2026 Oct 16;18(20):3392. doi: 10.3390/nu18203392. PMID- 42942953 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 15 TI - Dietary Sulfur Amino Acid Sensing and Metabolic Adaptation: From the GCN2-ATF4-FGF21 Axis to Transsulfuration and Redox Homeostasis. LID - 10.3390/nu18203382 [doi] LID - 3382 AB - Sulfur amino acids (SAAs), mainly methionine and cysteine, play an important role in coordinating nutrient sensing, one-carbon metabolism, redox regulation, and whole-body energy homeostasis. Dietary methionine restriction (MR) and sulfur amino acid restriction (SAAR) have gained increasing interest as nutritional interventions that produce pronounced metabolic benefits in preclinical models, including reductions in adiposity and hepatic lipid accumulation and improvements in insulin sensitivity and glucose regulation. Human intervention studies suggest biological activity and potential metabolic benefit, although the available clinical evidence remains limited and heterogeneous. These effects arise from the coordinated activation of multiple nutrient-responsive pathways. Reduced amino acid availability can stimulate the general control nonderepressible 2-eukaryotic translation initiation factor 2 alpha-activating transcription factor 4 signaling pathway (GCN2-eIF2alpha-ATF4) integrated stress response, whereas changes in methionine-derived S-adenosylmethionine modulate mechanistic target of rapamycin complex 1 (mTORC1) activity through S-adenosylmethionine sensor upstream of mTORC1 (SAMTOR)-dependent signaling. At the systemic level, hepatic fibroblast growth factor 21 contributes to the regulation of adipose tissue remodeling, energy expenditure, thermogenesis, and insulin sensitivity. At the same time, alterations in transsulfuration reshape cysteine utilization, glutathione metabolism, hydrogen sulfide signaling, and mitochondrial redox adaptation. Importantly, selective MR, broader SAAR, and total protein restriction should not be considered equivalent interventions, as their metabolic effects are influenced by cysteine availability and the degree of restriction. Nevertheless, the translation of these effects to humans remains incompletely established, and available intervention studies are limited in number, duration, and sample size. This review examines the biochemical and molecular mechanisms underlying SAA sensing and metabolic adaptation and evaluates their potential relevance to obesity, type 2 diabetes, metabolic syndrome, and metabolic dysfunction-associated steatotic liver disease, with particular emphasis on future applications in precision nutrition. FAU - Lucaciu, Roxana Liana AU - Lucaciu RL AUID- ORCID: 0000-0003-4920-4760 AD - Department of Pharmaceutical Biochemistry and Clinical Laboratory, Faculty of Pharmacy, "Iuliu-Hatieganu" University of Medicine and Pharmacy, 400349 Cluj-Napoca, Romania. FAU - Hangan, Adriana Corina AU - Hangan AC AUID- ORCID: 0000-0002-0944-3161 AD - Department of Inorganic Chemistry, Faculty of Pharmacy, "Iuliu-Hatieganu" University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. FAU - Procopciuc, Lucia Maria AU - Procopciuc LM AUID- ORCID: 0000-0002-4738-5775 AD - Department of Medical Biochemistry, "Iuliu-Hatieganu" University of Medicine and Pharmacy, 400349 Cluj-Napoca, Romania. LA - eng PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 62031-54-3 (Fibroblast Growth Factors) RN - 145891-90-3 (Activating Transcription Factor 4) RN - 0 (Amino Acids, Sulfur) RN - 0 (fibroblast growth factor 21) RN - EC 2.7.11.1 (Protein Serine-Threonine Kinases) RN - EC 2.7.11.1 (EIF2AK4 protein, human) RN - AE28F7PNPL (Methionine) RN - 0 (ATF4 protein, human) SB - IM MH - Humans MH - *Fibroblast Growth Factors/metabolism MH - *Activating Transcription Factor 4/metabolism MH - *Amino Acids, Sulfur/metabolism/administration & dosage MH - Homeostasis MH - Oxidation-Reduction MH - Animals MH - Signal Transduction MH - *Adaptation, Physiological MH - *Protein Serine-Threonine Kinases/metabolism MH - Methionine/metabolism MH - *Diet MH - Energy Metabolism MH - Integrated Stress Response PMC - PMC13708097 OTO - NOTNLM OT - FGF21 OT - GCN2-eIF2alpha-ATF4 OT - SAMTOR-mTORC1 OT - cysteine OT - metabolic adaptation OT - methionine OT - precision nutrition OT - redox homeostasis OT - sulfur amino acid restriction OT - sulfur amino acids COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/15 CRDT- 2026/10/27 10:43 PHST- 2026/09/19 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - nu18203382 [pii] AID - nutrients-18-03382 [pii] AID - 10.3390/nu18203382 [doi] PST - epublish SO - Nutrients. 2026 Oct 15;18(20):3382. doi: 10.3390/nu18203382. PMID- 42942951 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 15 TI - Effects of a Saccharomyces uvarum Cell-Based Preparation on Gut Microbial Ecology and Metabolic Activity in Adults Without Diagnosed Gastrointestinal Disease: A Randomized Placebo-Controlled Crossover Pilot Study. LID - 10.3390/nu18203380 [doi] LID - 3380 AB - Background/Objectives: Yeast-based probiotic candidates are increasingly investigated as potential modulators of gut ecology, although evidence in healthy individuals remains limited. This randomized, single-blind, crossover study primarily evaluated the tolerability of ATECREM Serius containing Saccharomyces uvarum SERIUS strain (>10(10) CFU/g) and explored its effect on human gut microbiota. Methods: Forty-four volunteers completed the crossover intervention consisting of 2 months of ATECREM Serius (TRT1) and 2 months of placebo (PLT1), separated by 2 weeks of wash-out. Fecal microbiota was profiled by 16S rRNA gene sequencing, while fecal and urinary volatile organic compounds (VOCs) were profiled by GC-MS. Fecal free amino acids (FAAs) were also measured. Results: ATECREM Serius was well tolerated, with no treatment-related adverse events. Differences were observed for three out of five alpha-diversity indices, while beta-diversity differed between TRT1 and PLT1. The relative abundance and prevalence of Bifidobacteriaceae and Bacteroides were increased in TRT1, whereas Enterobacteriaceae and clostridia sensu stricto were limited compared to placebo. Fecal metabolomics indicated an enrichment of organic acids, particularly SCFAs, in TRT1, whereas propanoic acid was reduced in PLT1. Fecal tryptophan and histidine decreased in TRT1, and urinary metabolomics showed a clearer clustering between TRT0 and TRT1, including a significant decrease of 2,5-dimethylfuran. Conclusions: In adults, ATECREM Serius was well tolerated within the investigated population and intervention period. Secondary exploratory analyses identified treatment-associated differences in selected gut microbial taxa and metabolomic features, including the increase in Bifidobacterium abundance compared to placebo. These findings are hypothesis-generating and require confirmation in larger, adequately powered studies. FAU - Vacca, Mirco AU - Vacca M AUID- ORCID: 0000-0003-0813-169X AD - Department of Soil, Plant and Food Science, University of Bari 'Aldo Moro', 70121 Bari, Italy. FAU - Khalil, Mohamad AU - Khalil M AUID- ORCID: 0000-0002-5943-9531 AD - Internal Medicine Clinica Medica "A. Murri", Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari 'Aldo Moro', 70121 Bari, Italy. FAU - Di Ciaula, Agostino AU - Di Ciaula A AUID- ORCID: 0000-0002-5476-7376 AD - Internal Medicine Clinica Medica "A. Murri", Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari 'Aldo Moro', 70121 Bari, Italy. FAU - Filannino, Pasquale AU - Filannino P AUID- ORCID: 0000-0002-1235-5138 AD - Department of Soil, Plant and Food Science, University of Bari 'Aldo Moro', 70121 Bari, Italy. FAU - Di Palo, Domenica Maria AU - Di Palo DM AD - Internal Medicine Clinica Medica "A. Murri", Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari 'Aldo Moro', 70121 Bari, Italy. FAU - Calabrese, Francesco Maria AU - Calabrese FM AUID- ORCID: 0000-0003-0176-7046 AD - Department of Soil, Plant and Food Science, University of Bari 'Aldo Moro', 70121 Bari, Italy. FAU - Bernardi, Matteo AU - Bernardi M AD - Department of Soil, Plant and Food Science, University of Bari 'Aldo Moro', 70121 Bari, Italy. FAU - Celano, Giuseppe AU - Celano G AUID- ORCID: 0000-0002-3805-7681 AD - Department of Soil, Plant and Food Science, University of Bari 'Aldo Moro', 70121 Bari, Italy. FAU - Iacobellis, Ilaria AU - Iacobellis I AD - Department of Soil, Plant and Food Science, University of Bari 'Aldo Moro', 70121 Bari, Italy. FAU - Garruti, Gabriella AU - Garruti G AUID- ORCID: 0000-0001-7875-7239 AD - Section of Internal Medicine, Endocrinology, Andrology and Metabolic Diseases, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari 'Aldo Moro', 70121 Bari, Italy. FAU - Ripari, Valery AU - Ripari V AD - Bioenologia 2.0 S.r.l., 31046 Oderzo, Italy. FAU - Teardo, Enrico AU - Teardo E AD - Bioenologia 2.0 S.r.l., 31046 Oderzo, Italy. FAU - Polo, Maurizio AU - Polo M AD - Bioenologia 2.0 S.r.l., 31046 Oderzo, Italy. FAU - De Angelis, Maria AU - De Angelis M AUID- ORCID: 0000-0002-2010-884X AD - Department of Soil, Plant and Food Science, University of Bari 'Aldo Moro', 70121 Bari, Italy. FAU - Portincasa, Piero AU - Portincasa P AUID- ORCID: 0000-0001-5359-1471 AD - Internal Medicine Clinica Medica "A. Murri", Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari 'Aldo Moro', 70121 Bari, Italy. LA - eng GR - No. 1550 of 11/10/2022/Ministry of Universities and Research/ PT - Journal Article PT - Randomized Controlled Trial DEP - 20261015 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Amino Acids) RN - 0 (Volatile Organic Compounds) SB - IM MH - Humans MH - Cross-Over Studies MH - *Gastrointestinal Microbiome MH - *Saccharomyces MH - Feces/microbiology/chemistry MH - Adult MH - *Probiotics/administration & dosage MH - Male MH - Pilot Projects MH - Female MH - Middle Aged MH - Single-Blind Method MH - Amino Acids MH - Volatile Organic Compounds MH - Young Adult PMC - PMC13708381 OTO - NOTNLM OT - Saccharomyces uvarum OT - crossover clinical trial OT - gut microbiota OT - metabolomics OT - probiotic yeast OT - short-chain fatty acids COIS- Valery Ripari (V.R.), Enrico Teardo (E.T.), and Maurizio Polo (M.P.) are employed by the company Bioenologia 2.0 (Oderzo, Italy). The remaining authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/15 CRDT- 2026/10/27 10:43 PHST- 2026/06/25 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - nu18203380 [pii] AID - nutrients-18-03380 [pii] AID - 10.3390/nu18203380 [doi] PST - epublish SO - Nutrients. 2026 Oct 15;18(20):3380. doi: 10.3390/nu18203380. PMID- 42942948 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 15 TI - From Pollen to Plate: A Botanical Classification-Based Narrative Review of Fresh Fruit Allergy. LID - 10.3390/nu18203377 [doi] LID - 3377 AB - Fruit consumption is a cornerstone of a healthy diet, providing essential micronutrients and bioactive compounds; however, fresh fruits may also trigger IgE-mediated allergic reactions, ranging from mild oral symptoms to potentially life-threatening anaphylaxis. Fruit allergy is a heterogeneous field characterized by diverse clinical phenotypes, botanical relationships, molecular sensitization profiles, and patterns of cross-reactivity. This narrative review organizes the available evidence according to 18 botanical families, adapting and extending an established botanical classification framework to the specific context of fresh fruit allergy. Botanical classification serves as a structured organizational framework rather than as a predictor of allergenic cross-reactivity, which is primarily determined by shared or homologous allergenic proteins and may occur both within and across botanical families. The review integrates botanical relationships with clinical manifestations, molecular allergen families, cross-reactivity, cofactors, diagnosis, and management. Clinical assessment should be individualized and based on the clinical history and appropriate in vivo and in vitro testing, including skin prick or prick-to-prick testing with fresh fruit and measurement of specific IgE, with oral food challenges reserved for selected cases. Molecular allergology may provide additional information for risk assessment in selected patients, although the availability and strength of evidence for individual molecular components vary considerably. Management should likewise be individualized according to the confirmed trigger, clinical phenotype, and risk of systemic reactions, with appropriate dietary counselling and patient education. The available epidemiological and clinical evidence is heterogeneous, particularly for uncommon fruits and in pediatric populations. It should therefore be interpreted in the context of study design, geographic setting, and diagnostic approach. Integrating botanical classification with molecular allergology and clinical phenotyping may provide a useful framework for organizing the fragmented evidence on fresh fruit allergy and informing individualized diagnostic and management approaches. FAU - Pecoraro, Luca AU - Pecoraro L AUID- ORCID: 0000-0002-9765-8006 AD - Department of Experimental Medicine, Pediatric Section, University of Salento, Hospital "Vito Fazzi", 73100 Lecce, Italy. FAU - Mastrorilli, Carla AU - Mastrorilli C AD - Pediatric Hospital Giovanni XXIII, Pediatric and Emergency Department, AOU Policlinic of Bari, 70100 Bari, Italy. FAU - Castagnoli, Riccardo AU - Castagnoli R AUID- ORCID: 0000-0003-0029-9383 AD - Pediatric Clinic, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy. FAU - Mori, Francesca AU - Mori F AUID- ORCID: 0000-0001-7483-0128 AD - Allergy Unit, Meyer Children's Hospital IRCCS, 50139 Florence, Italy. FAU - Arasi, Stefania AU - Arasi S AUID- ORCID: 0000-0002-8135-0568 AD - Allergy Diseases Research Area, Pediatric Allergology Unit, Bambino Gesu Children's Hospital, IRCCS, 00146 Rome, Italy. FAU - Klain, Angela AU - Klain A AD - Department of Woman, Child and General and Specialized Surgery, University of Campania Luigi Vanvitelli, 80100 Naples, Italy. FAU - Liotti, Lucia AU - Liotti L AD - Pediatric Unit, Department of Mother and Child Health, Salesi Children's Hospital, 60123 Ancona, Italy. FAU - Barni, Simona AU - Barni S AD - Allergy Unit, Meyer Children's Hospital IRCCS, 50139 Florence, Italy. FAU - Gelsomino, Mariannita AU - Gelsomino M AD - Pediatric Allergy Unit, Department of Life Sciences and Public Health, Fondazione Policlinico Universitario A. Gemelli IRCCS, Catholic University of the Sacred Heart, 00168 Rome, Italy. FAU - Giovannini, Mattia AU - Giovannini M AUID- ORCID: 0000-0001-9568-6882 AD - Allergy Unit, Meyer Children's Hospital IRCCS, 50139 Florence, Italy. AD - Department of Health Sciences, University of Florence, 50139 Florence, Italy. FAU - Caminiti, Lucia AU - Caminiti L AD - Department of Human Pathology in Adult and Development Age "Gaetano Barresi", Allergy Unit, Department of Pediatrics, AOU Policlinico Gaetano Martino, 98100 Messina, Italy. FAU - Indolfi, Cristiana AU - Indolfi C AUID- ORCID: 0000-0002-9513-193X AD - Department of Woman, Child and General and Specialized Surgery, University of Campania Luigi Vanvitelli, 80100 Naples, Italy. FAU - Saretta, Francesca AU - Saretta F AD - Pediatric Department, Latisana-Palmanova Hospital, Azienda Sanitaria Universitaria Friuli Centrale, 33100 Udine, Italy. FAU - Marseglia, Gian Luigi AU - Marseglia GL AD - Pediatric Clinic, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy. FAU - Novembre, Elio AU - Novembre E AD - Department of Health Sciences, University of Florence, 50139 Florence, Italy. CN - Rare Allergic Diseases Commission of the Italian Society of Pediatric Allergy and Immunology (SIAIP) LA - eng PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Allergens) RN - 37341-29-0 (Immunoglobulin E) SB - IM MH - Humans MH - *Fruit/immunology/adverse effects/classification MH - *Food Hypersensitivity/immunology/diagnosis MH - *Allergens/immunology MH - Immunoglobulin E/immunology/blood MH - Cross Reactions MH - *Pollen/immunology MH - Skin Tests PMC - PMC13708038 OTO - NOTNLM OT - anaphylaxis OT - botanical classification OT - component-resolved diagnostics OT - cross-reactivity OT - fruit allergy OT - gibberellin-regulated proteins OT - lipid transfer proteins OT - oral allergy syndrome OT - oral food challenge OT - skin prick test OT - specific IgE COIS- M.G. (Mattia Giovannini) reports personal fees from Sanofi, Thermo Fisher Scientific. The other authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/15 CRDT- 2026/10/27 10:43 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - nu18203377 [pii] AID - nutrients-18-03377 [pii] AID - 10.3390/nu18203377 [doi] PST - epublish SO - Nutrients. 2026 Oct 15;18(20):3377. doi: 10.3390/nu18203377. PMID- 42942944 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 14 TI - Adaptive Precision Nutrition: Integrating Dynamic Phenotyping, Multi-Omics, Artificial Intelligence, and the Allostatic Viability Principle. LID - 10.3390/nu18203373 [doi] LID - 3373 AB - Precision nutrition increasingly combines functional foods, multi-omics, digital phenotyping, and artificial intelligence, yet the incremental decision value of additional data layers remains uncertain. This critical narrative review and hypothesis-generating conceptual proposal examines evidence on personalized nutrition, dynamic response phenotyping, functional-food heterogeneity, multi-omics integration, and AI-supported decisions. Personalized advice can improve dietary behavior and selected cardiometabolic outcomes, whereas the additional clinical benefit attributable specifically to elaborate molecular profiling has been inconsistent. Dynamic challenge studies suggest that response amplitude, timing, coordination, recovery, and conditional repeatability may provide information beyond static profiles, but reproducibility is required before these features can be treated as person-specific traits. The Allostatic Viability Principle (AVP) is proposed as a conceptual physiological and decision framework rather than a validated predictor or biomarker. Its intended contribution is to organize existing approaches around viable, context-appropriate regulation, proportional use of additional measurements, explicit uncertainty, an outcome hierarchy that does not equate molecular change with health benefit, and a response-and-revision loop. Whether this organization improves decisions or outcomes beyond high-quality conventional or adaptive personalized nutrition remains an empirical question. The framework is therefore presented as a testable structure for determining when additional individual-level information is reproducible, relevant, and sufficiently decision changing to justify use. FAU - Haslberger, Alexander G AU - Haslberger AG AUID- ORCID: 0000-0001-9699-537X AD - Department of Nutritional Sciences, Faculty of Life Sciences, University of Vienna, Josef-Holaubek-Platz 2 (UZA II), 1090 Vienna, Austria. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 SB - IM MH - Humans MH - *Artificial Intelligence MH - Multiomics MH - *Precision Medicine/methods MH - Phenotype MH - *Allostasis PMC - PMC13708328 OTO - NOTNLM OT - adaptive capacity OT - allostasis OT - artificial intelligence OT - functional foods OT - multi-omics OT - nutrigenomics OT - personalized nutrition OT - phenotypic flexibility OT - precision nutrition OT - viability COIS- The author declares no conflict of interest. The author is involved in the development of precision-health concepts and commercial health-platform activities related to personalized prevention. These activities had no role in the selection or interpretation of the literature presented in this review. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/14 CRDT- 2026/10/27 10:43 PHST- 2026/08/24 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - nu18203373 [pii] AID - nutrients-18-03373 [pii] AID - 10.3390/nu18203373 [doi] PST - epublish SO - Nutrients. 2026 Oct 14;18(20):3373. doi: 10.3390/nu18203373. PMID- 42942940 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 14 TI - Oligosaccharides, Gut Microbiota, and Mucosal Health: Molecular Mechanisms and Implications for Early-Life Gut Health. LID - 10.3390/nu18203369 [doi] LID - 3369 AB - Oligosaccharides are structurally diverse glycans that influence intestinal homeostasis through mechanisms extending beyond conventional prebiotic activity. This review examines how dietary, human milk, host-derived, and biotechnologically produced oligosaccharides modulate the gut microbiota and mucosal host-microbe interactions, with particular attention to pediatric health. Non-digestible oligosaccharides support saccharolytic microbial communities, cross-feeding networks, and short-chain fatty acid production, thereby affecting luminal pH, epithelial metabolism, mucus production, barrier integrity, and immune signaling. Their biological effects are strongly determined by monosaccharide composition, linkage configuration, branching, charge, degree of polymerization, terminal residues, and spatial presentation. Neutral human milk oligosaccharides and galacto-oligosaccharides may reduce pathogen colonization by mimicking host glycans, occupying adhesin-binding sites, modifying epithelial glycosylation, and limiting biofilm initiation. Selected chitooligosaccharides can exert direct antibacterial effects through charge-dependent interactions with bacterial envelopes, although these effects depend on molecular size, charge, and chemical derivatization. In addition, the alpha1,4-GlcNAc-terminated O-glycan motif of gastric MUC6 provides a distinct example of host-associated glycan-mediated inhibition of bacterial metabolism. During infancy, human milk oligosaccharides promote a Bifidobacterium-enriched microbiota and may contribute to intestinal and immune maturation. Galacto- and fructo-oligosaccharide-supplemented formulas reproduce some microbiota- and metabolite-related effects, although evidence for preventing infectious, allergic, or inflammatory disease remains inconsistent. Experimental findings also suggest a role in recovery from antibiotic-associated dysbiosis, but clinical evidence is limited by heterogeneous populations, exposures, formulations, and endpoints. Future studies should use structurally defined compounds, mechanism-specific assays, and clinically relevant outcomes to establish causal structure-function relationships and identify pediatric populations most likely to benefit. FAU - Mentino, Donatella AU - Mentino D AUID- ORCID: 0000-0002-0680-5251 AD - Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy. FAU - Scillitani, Giovanni AU - Scillitani G AUID- ORCID: 0000-0002-5888-7906 AD - Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy. FAU - Semeraro, Daniela AU - Semeraro D AUID- ORCID: 0000-0003-4447-3910 AD - Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy. FAU - Guglielmi, Marco Vito AU - Guglielmi MV AUID- ORCID: 0000-0003-1296-861X AD - Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy. FAU - Taldone, Nastasia AU - Taldone N AUID- ORCID: 0009-0000-5144-7150 AD - Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy. FAU - Laterza, Michelle AU - Laterza M AD - Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy. FAU - Vitale, Rossella AU - Vitale R AD - Pediatric Unit, Department of Precision and Regenerative Medicine and Ionian Area, University of Bari Aldo Moro, 70124 Bari, Italy. FAU - Faienza, Maria Felicia AU - Faienza MF AUID- ORCID: 0000-0002-1899-8337 AD - Pediatric Unit, Department of Precision and Regenerative Medicine and Ionian Area, University of Bari Aldo Moro, 70124 Bari, Italy. FAU - Mastrodonato, Maria AU - Mastrodonato M AUID- ORCID: 0000-0002-0799-8032 AD - Department of Biosciences, Biotechnology and Environment, University of Bari Aldo Moro, 70125 Bari, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Oligosaccharides) RN - 0 (Prebiotics) SB - IM MH - Humans MH - *Oligosaccharides/metabolism/pharmacology MH - *Gastrointestinal Microbiome/physiology/drug effects MH - *Intestinal Mucosa/microbiology/metabolism/physiology MH - Milk, Human/chemistry MH - Prebiotics MH - Infant MH - Intestinal Barrier Function PMC - PMC13708204 OTO - NOTNLM OT - bacterial adhesion OT - gut microbiota OT - human milk oligosaccharides OT - intestinal mucus OT - mucosal barrier OT - oligosaccharides OT - pediatric nutrition COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/14 CRDT- 2026/10/27 10:43 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - nu18203369 [pii] AID - nutrients-18-03369 [pii] AID - 10.3390/nu18203369 [doi] PST - epublish SO - Nutrients. 2026 Oct 14;18(20):3369. doi: 10.3390/nu18203369. PMID- 42942939 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 14 TI - Human Milk Oligosaccharides in Sports Nutrition: Biological Rationale and Current Evidence. LID - 10.3390/nu18203367 [doi] LID - 3367 AB - Established sports-nutrition strategies support fueling, hydration, and muscle protein turnover, but strenuous exercise also challenges gastrointestinal, microbial, immune, metabolic, and musculoskeletal homeostasis. Human milk oligosaccharides (HMOs) are structurally diverse bioactive glycans that can selectively influence gut microbial composition, microbial metabolism, intestinal barrier integrity, and immune function. These properties provide a biological rationale for investigating HMOs in exercise and sports nutrition. This review critically evaluates direct and supportive evidence linking the biological functions of HMOs to exercise physiology. Human evidence is limited to two small trials. One examined supplementation alongside dietary restriction and increased physical activity, limiting conclusions about exercise adaptation; the other found no additional gains in muscle mass, strength, or anaerobic performance during resistance training. Alongside the emerging exercise-related findings, the established effects of HMOs on the gut microbiota and intestinal barrier also suggest a potential role in reducing exercise-associated gastrointestinal disturbances. However, benefits for these disturbances or post-exercise recovery have not been established. HMOs remain candidates for complementing established sports-nutrition strategies, but current evidence is insufficient to recommend supplementation for athletes. Future studies should determine how HMO structure, dose, formulation, and host characteristics influence gastrointestinal resilience, musculoskeletal adaptation, and functional outcomes in physically active populations. Establishing these relationships will be essential for translating HMOs into evidence-based sports-nutrition applications. FAU - Hu, Ke AU - Hu K AD - Department of Health and Physical Education, Institute of Intelligent Sport and Proactive Health, Jianghan University, Wuhan 430056, China. FAU - Zhang, Kaimeng AU - Zhang K AD - Department of Health and Physical Education, Institute of Intelligent Sport and Proactive Health, Jianghan University, Wuhan 430056, China. FAU - Huang, Chenxi AU - Huang C AUID- ORCID: 0000-0002-4485-8071 AD - Synaura Biotechnology (Shanghai) Co., Ltd., Shanghai 201210, China. FAU - Wang, Zhaoguan AU - Wang Z AD - School of Optoelectronic Materials and Technology, Jianghan University, Wuhan 430056, China. FAU - Huang, Xuanxuan AU - Huang X AD - Department of Health and Physical Education, Institute of Intelligent Sport and Proactive Health, Jianghan University, Wuhan 430056, China. FAU - Li, Yin AU - Li Y AD - Department of Health and Physical Education, Institute of Intelligent Sport and Proactive Health, Jianghan University, Wuhan 430056, China. FAU - Cheng, Lianghui AU - Cheng L AUID- ORCID: 0000-0002-9778-8230 AD - Department of Health and Physical Education, Institute of Intelligent Sport and Proactive Health, Jianghan University, Wuhan 430056, China. FAU - Wu, Yuxiang AU - Wu Y AUID- ORCID: 0000-0002-9041-4471 AD - Department of Health and Physical Education, Institute of Intelligent Sport and Proactive Health, Jianghan University, Wuhan 430056, China. AD - School of Optoelectronic Materials and Technology, Jianghan University, Wuhan 430056, China. LA - eng GR - 2025300/Hubei Provincial Department of Education/ PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Oligosaccharides) SB - IM MH - Humans MH - *Oligosaccharides/administration & dosage/pharmacology MH - *Milk, Human/chemistry MH - *Sports Nutritional Physiological Phenomena MH - Dietary Supplements MH - Exercise/physiology MH - Gastrointestinal Microbiome/drug effects MH - *Sports Nutritional Sciences MH - Athletes PMC - PMC13708281 OTO - NOTNLM OT - exercise OT - human milk oligosaccharides OT - recovery OT - sports nutrition COIS- The authors declare no conflicts of interest. Chenxi Huang is employed by Synaura Biotechnology (Shanghai) Co., Ltd. The company provided no funding or materials for this review and had no role in its conception, literature selection, interpretation of the evidence, manuscript preparation, or the decision to publish. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/14 CRDT- 2026/10/27 10:43 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - nu18203367 [pii] AID - nutrients-18-03367 [pii] AID - 10.3390/nu18203367 [doi] PST - epublish SO - Nutrients. 2026 Oct 14;18(20):3367. doi: 10.3390/nu18203367. PMID- 42942937 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 14 TI - Berberine as a Mitochondrial Metabolic Reprogrammer in Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD): Adaptive Energetic Stress, Quality Control, and Translational Limitations. LID - 10.3390/nu18203366 [doi] LID - 3366 AB - Metabolic dysfunction-associated steatotic liver disease (MASLD) is closely linked to mitochondrial dysfunction, including altered oxidative phosphorylation, disrupted fatty-acid oxidation, redox imbalance, abnormal mitochondrial dynamics, and impaired quality control. Berberine (BBR), a plant-derived isoquinoline alkaloid used as a nutraceutical, has been described both as a partial inhibitor of respiratory-chain complex I and as an intervention that improves mitochondrial function. This narrative review critically synthesizes evidence from hepatoma cells, primary hepatocytes, animal models, and human studies to reconcile these divergent effects. The preclinical literature supports a proposed, context-dependent model in which BBR imposes an energetic constraint during nutrient overload while activating AMP-activated protein kinase- and sirtuin-linked pathways that remodel substrate handling, respiration, redox balance, mitochondrial architecture, and autophagy. The strongest mechanistic evidence supports complex I-associated respiratory remodeling, SIRT3-mediated deacetylation of long-chain acyl-CoA dehydrogenase, SIRT1-mediated stabilization of carnitine palmitoyltransferase 1A, and SIRT1-dependent autophagy. Evidence for consistent mitochondrial biogenesis, selective mitophagy, mitochondria-endoplasmic reticulum contact-site remodeling, mitochondrial DNA danger signaling, and ferroptosis regulation in MASLD remains limited. Human evidence is mixed: a recent placebo-controlled trial found no significant reduction in liver fat with pure BBR, and no included trial directly measured hepatic mitochondrial function or established a mitochondria-mediated clinical effect. Therefore, BBR is best viewed as a context-dependent modulator of hepatic mitochondrial metabolism rather than a uniform mitochondrial enhancer. Pure BBR should not be conflated with whole barberry fruit, uncharacterized botanical preparations, or berberine ursodeoxycholate (HTD1801). Translation requires characterized formulations, exposure-matched models, and direct mitochondria-focused endpoints. FAU - Rafiei, Hossein AU - Rafiei H AUID- ORCID: 0000-0002-4843-9261 AD - Department of Nutrition and Dietetics, Saint Louis University, Saint Louis, MO 63104, USA. FAU - Omidian, Kosar AU - Omidian K AD - College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, SK S7N 2Z4, Canada. FAU - Tolentino, Miguel AU - Tolentino M AD - Department of Nutrition and Dietetics, Saint Louis University, Saint Louis, MO 63104, USA. FAU - Jafari, Ali AU - Jafari A AUID- ORCID: 0000-0001-7343-027X AD - Student Research Committee, Department of Community Nutrition, Faculty of Nutrition Sciences and Food Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran 1467864763, Iran. FAU - Bahari, Hossein AU - Bahari H AUID- ORCID: 0000-0002-3787-2713 AD - Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 9919191778, Iran. FAU - Khalafi, Mousa AU - Khalafi M AD - Department of Sport Sciences, Faculty of Humanities, University of Kashan, Kashan 8731753153, Iran. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0I8Y3P32UF (Berberine) SB - IM MH - *Berberine/pharmacology/therapeutic use MH - Humans MH - Animals MH - *Fatty Liver/metabolism/drug therapy MH - *Energy Metabolism/drug effects MH - *Non-alcoholic Fatty Liver Disease/drug therapy/metabolism MH - Metabolic Reprogramming MH - Quality Control MH - *Mitochondria, Liver/metabolism/drug effects MH - *Mitochondria/drug effects/metabolism MH - *Metabolic Diseases/drug therapy PMC - PMC13708704 OTO - NOTNLM OT - berberine OT - metabolic dysfunction-associated steatotic liver disease OT - mitochondrial function OT - mitochondrial quality control OT - nonalcoholic fatty liver disease OT - oxidative phosphorylation OT - oxidative stress OT - sirtuins COIS- The authors declare no conflicts of interest. The authors hold no intellectual-property interests related to berberine and have no supplement-industry, commercial, or financial relationships related to berberine-containing products. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/14 CRDT- 2026/10/27 10:43 PHST- 2026/09/06 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - nu18203366 [pii] AID - nutrients-18-03366 [pii] AID - 10.3390/nu18203366 [doi] PST - epublish SO - Nutrients. 2026 Oct 14;18(20):3366. doi: 10.3390/nu18203366. PMID- 42942930 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 14 TI - Fructose Promotes Oxidative Stress, Sphingolipid Remodelling and Transient Intestinal Barrier Dysfunction in Differentiated Caco-2 Cells. LID - 10.3390/nu18203359 [doi] LID - 3359 AB - Background/Objectives: High dietary fructose consumption has been linked to impaired intestinal barrier function and several metabolic disorders. Although oxidative stress has been proposed as a mediator of fructose-related epithelial injury, the involvement of tight-junction (TJ) proteins and sphingolipid metabolism is still not fully defined. Here, differentiated Caco-2 monolayers were exposed to fructose (1-50 mM) to examine changes in epithelial barrier integrity and associated molecular responses. Methods: Cell viability, transepithelial electrical resistance (TEER), reactive oxygen species (ROS), intracellular reduced and oxidized glutathione (GSH/GSSG) ratio, nitrites as an indirect assessment of nitric oxide (NO) production, TJ protein level (ZO-1, JAM-A and occludin), sphingolipid profile by UHPLC-QTOF-MS, and expression of the ceramide synthases CERS2 and CERS6 were evaluated. Results: Fructose did not affect cell viability but induced a rapid, concentration-dependent increase in ROS generation, an early reduction in the GSH/GSSG ratio, and increased nitrites production. The observed alterations were concurrent with a temporary reduction in TEER, which returned to control levels at subsequent time points. Fructose selectively reduced JAM-A and ZO-1 protein expression, whereas occludin remained unaffected. Lipidomic analysis revealed sphingolipid remodelling, characterised by increased ceramide species and reduced sphingomyelins, while gene expression analysis showed downregulation of CERS2, with CERS6 remaining unchanged. The altered sphingolipid profile mainly involved long- and very-long-chain ceramides, suggesting a possible link between altered membrane lipid homeostasis and barrier dysfunction. Conclusions: Fructose exposure transiently compromises intestinal epithelial barrier function and is associated with oxidative stress, changes in TJ proteins, and sphingolipid remodelling. These preliminary findings identify sphingolipid remodelling as a candidate contributor to fructose-induced intestinal barrier dysfunction, warranting confirmation through dedicated mechanistic follow-up studies. FAU - Serreli, Gabriele AU - Serreli G AUID- ORCID: 0000-0002-1971-3989 AD - Department of Biomedical Sciences, University of Cagliari, 09042 Monserrato, Italy. FAU - Melis, Maria Paola AU - Melis MP AUID- ORCID: 0000-0003-0259-6103 AD - Department of Biomedical Sciences, University of Cagliari, 09042 Monserrato, Italy. FAU - Guerriero, Claudia AU - Guerriero C AD - Department of Biomedical Sciences, University of Cagliari, 09042 Monserrato, Italy. FAU - Manis, Cristina AU - Manis C AUID- ORCID: 0000-0001-8091-7908 AD - Department of Life and Environmental Sciences, University of Cagliari, 09042 Monserrato, Italy. FAU - Tolle, Giulia AU - Tolle G AD - Department of Life and Environmental Sciences, University of Cagliari, 09042 Monserrato, Italy. FAU - Sanna, Giuseppina AU - Sanna G AUID- ORCID: 0000-0002-3999-4263 AD - Department of Biomedical Sciences, University of Cagliari, 09042 Monserrato, Italy. FAU - Orru, Germano AU - Orru G AUID- ORCID: 0000-0003-1032-4431 AD - Oral Biotechnology Laboratory (OBL), Department of Surgical Sciences, University of Cagliari, 09121 Cagliari, Italy. FAU - Fais, Sara AU - Fais S AD - Oral Biotechnology Laboratory (OBL), Department of Surgical Sciences, University of Cagliari, 09121 Cagliari, Italy. FAU - Caboni, Pierluigi AU - Caboni P AUID- ORCID: 0000-0003-2448-3767 AD - Department of Life and Environmental Sciences, University of Cagliari, 09042 Monserrato, Italy. FAU - Deiana, Monica AU - Deiana M AUID- ORCID: 0000-0002-7166-1856 AD - Department of Biomedical Sciences, University of Cagliari, 09042 Monserrato, Italy. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Sphingolipids) RN - 30237-26-4 (Fructose) RN - 0 (Reactive Oxygen Species) RN - 31C4KY9ESH (Nitric Oxide) RN - GAN16C9B8O (Glutathione) SB - IM MH - Humans MH - *Oxidative Stress/drug effects MH - Caco-2 Cells MH - *Sphingolipids/metabolism MH - *Intestinal Barrier Function/drug effects MH - *Fructose/adverse effects/pharmacology MH - Reactive Oxygen Species/metabolism MH - Cell Survival/drug effects MH - Cell Differentiation/drug effects MH - *Intestinal Mucosa/drug effects/metabolism MH - Nitric Oxide/metabolism MH - Tight Junctions/metabolism/drug effects MH - Glutathione/metabolism MH - Electric Impedance PMC - PMC13708186 OTO - NOTNLM OT - Caco-2 cells OT - ceramides OT - fructose OT - intestinal barrier OT - oxidative stress OT - sphingolipid metabolism OT - tight junctions COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/14 CRDT- 2026/10/27 10:43 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - nu18203359 [pii] AID - nutrients-18-03359 [pii] AID - 10.3390/nu18203359 [doi] PST - epublish SO - Nutrients. 2026 Oct 14;18(20):3359. doi: 10.3390/nu18203359. PMID- 42942929 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 14 TI - Effects of Yeast Protein-Whey Protein Composite Supplementation on Recovery from Exercise-Induced Muscle Damage in Well-Trained Athletes: A Randomized Controlled Trial. LID - 10.3390/nu18203358 [doi] LID - 3358 AB - Objectives: Exercise-induced muscle damage (EIMD) can impair muscle function and subsequent athletic performance. This study examined whether an isonitrogenous yeast protein-whey protein composite (YP) modifies recovery from EIMD compared with whey protein (WP) and a water-only control (C) in well-trained athletes. Methods: In this prospective, randomized, three-arm trial, 54 athletes were allocated to YP, WP, or C; 48 completed all follow-up procedures. YP and WP provided 0.9 g.kg(-1).day(-1) of actual protein, whereas C received water only and was not energy or sensory matched to the protein interventions. After 7 days of supplementation, participants completed a bilateral isokinetic EIMD protocol; supplementation continued through the 48 h assessment. Outcomes were assessed before EIMD, immediately afterward, and at 24, 48, and 72 h and were organized into muscle damage and soreness, inflammatory responses, and muscle function and performance. Female and male athletes were analyzed separately using linear mixed-effects models with multiplicity-adjusted contrasts. The study was not powered to formally compare intervention effects between sexes. Results: EIMD produced time-dependent biochemical, perceptual, and functional changes. Holm-adjusted comparisons showed lower CK in YP than C at 24 h in females and 48 h in males. The female 24 h CK finding was considered exploratory because the corresponding group x time interaction did not remain significant after damage-domain BH-FDR correction; the immediate male YP-C difference was significant only in the log-scale analysis. In males, YP was associated with lower soreness averaged across assessments and a lower immediate post-EIMD IL-6 value than C. Selected male CMJ comparisons favored YP but were exploratory after broader multiplicity correction. Acute-decline analyses showed smaller losses with YP in bilateral MVC and right-leg eccentric peak torque in males; only the right-leg eccentric YP-C comparison remained significant after BH-FDR correction. Conclusions: Compared with water alone, YP favorably influenced selected biochemical, perceptual, and muscle-function responses following EIMD, with the clearest pattern observed in the male analysis. Its overall response pattern was broadly aligned with WP, supporting the recovery-related potential and practical value of incorporating yeast-derived protein into a whey-based formulation. FAU - Xu, Chunyan AU - Xu C AUID- ORCID: 0000-0002-9080-1471 AD - Department of Sport Science Institute, Beijing Sport University, Beijing 100084, China. AD - Beijing Higher School Engineering Research Center of Sport Nutrition, Beijing Sport University, Beijing 100084, China. AD - Laboratory of Sports Stress and Adaptation of General Administration of Sport, Beijing Sport University, Beijing 100084, China. FAU - Gong, Lijing AU - Gong L AUID- ORCID: 0000-0003-3502-0901 AD - China Institute of Sport and Health Science, Beijing Sport University, Beijing 100084, China. AD - Key Laboratory for Performance Training & Recovery of General Administration of Sport, Beijing 100084, China. FAU - Ni, Zhen AU - Ni Z AD - China Institute of Sport and Health Science, Beijing Sport University, Beijing 100084, China. FAU - Wang, Xintang AU - Wang X AUID- ORCID: 0009-0007-5659-4556 AD - China Institute of Sport and Health Science, Beijing Sport University, Beijing 100084, China. LA - eng GR - No. BSU20240128/Beijing Sport University/ PT - Journal Article PT - Randomized Controlled Trial DEP - 20261014 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Whey Proteins) RN - EC 2.7.3.2 (Creatine Kinase) SB - IM MH - Humans MH - Male MH - *Whey Proteins/administration & dosage MH - *Dietary Supplements MH - Female MH - *Athletes MH - *Myalgia/etiology MH - Post-Exercise Recovery MH - Prospective Studies MH - *Muscle, Skeletal/physiopathology/drug effects MH - Young Adult MH - Adult MH - Post-Exercise Recovery Techniques MH - *Exercise MH - Creatine Kinase/blood MH - Adolescent PMC - PMC13708182 OTO - NOTNLM OT - athletes OT - creatine kinase OT - exercise-induced muscle damage OT - muscle function OT - muscle soreness OT - whey protein OT - yeast protein COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/14 CRDT- 2026/10/27 10:43 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - nu18203358 [pii] AID - nutrients-18-03358 [pii] AID - 10.3390/nu18203358 [doi] PST - epublish SO - Nutrients. 2026 Oct 14;18(20):3358. doi: 10.3390/nu18203358. PMID- 42942928 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 13 TI - Effectiveness of mHealth Nutrition Interventions on Nutrition-Related and Clinical Outcomes in Adults with Chronic Kidney Disease: A Systematic Review and Meta-Analysis. LID - 10.3390/nu18203357 [doi] LID - 3357 AB - BACKGROUND/OBJECTIVES: Diet recommendations for individuals with chronic kidney disease (CKD) are complex and can be challenging to integrate into daily routines. Mobile health (mHealth) interventions may support dietary education, self-monitoring, and disease management; however, their effects on nutrition-related and clinical outcomes in CKD remain unclear. This systematic review and meta-analysis evaluated the effectiveness of mHealth interventions on nutrition-related and clinical outcomes among adults with CKD. METHODS: The review was conducted according to the Preferred Reporting Items for Systematic Review and Meta-analysis (PRISMA) guidelines. Five databases were searched from inception through February 2026. Controlled trials, quasi-experimental designs and pre-post studies evaluating mHealth interventions and reporting nutrition-related and/or clinical outcomes were eligible for inclusion. Randomized controlled trials and controlled quasi-experimental studies with comparable outcomes and sufficient data were included in the quantitative synthesis. Pre-post studies were synthesized narratively because changes from baseline could not be distinguished from temporal effects in the absence of a concurrent comparator. Risk of bias was assessed using the Cochrane Risk of Bias tool and ROBINS-I. RESULTS: Twenty-three studies were included in the review, with eight meeting the inclusion criteria for a meta-analysis. Among the quantitative synthesis of adults on dialysis, no significant effects were shown with biochemical outcomes or interdialytic weight gain. Several pre-post studies reported within-study improvements in dietary intake, including sodium intake, and blood pressure outcomes. These findings should be interpreted cautiously because the absence of a concurrent control group precludes attributing observed changes specifically to the mHealth intervention. CONCLUSIONS: mHealth interventions may support improvements in selected dietary and clinical outcomes among adults with CKD; however, evidence from randomized controlled trials remains limited. Improvements reported in pre/post studies should be interpreted cautiously and additional well-designed randomized controlled trials are needed to determine their effectiveness across CKD populations. FAU - Walter-Cano, Samantha AU - Walter-Cano S AD - Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA. FAU - Lee, Allison AU - Lee A AD - Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA. FAU - Andrade, Jeanette M AU - Andrade JM AUID- ORCID: 0000-0003-4452-4546 AD - Food Science and Human Nutrition Department, University of Florida, Gainesville, FL 32611, USA. LA - eng GR - 2022-67037-36614/United States Department of Agriculture - National Institute of Food and Agriculture/ PT - Journal Article PT - Meta-Analysis PT - Review PT - Systematic Review DEP - 20261013 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 SB - IM MH - Humans MH - *Renal Insufficiency, Chronic/therapy/diet therapy MH - *Telemedicine MH - Digital Health MH - Adult MH - Nutritional Status MH - Treatment Outcome MH - Female MH - Male MH - Middle Aged PMC - PMC13708690 OTO - NOTNLM OT - chronic kidney disease OT - clinical outcomes OT - mHealth OT - nutrition-related outcomes OT - systematic review and meta-analysis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/13 CRDT- 2026/10/27 10:43 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - nu18203357 [pii] AID - nutrients-18-03357 [pii] AID - 10.3390/nu18203357 [doi] PST - epublish SO - Nutrients. 2026 Oct 13;18(20):3357. doi: 10.3390/nu18203357. PMID- 42942921 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 13 TI - Cold Exposure-Induced Intestinal Dysfunction: Mechanisms, Animal Models, and Nutritional Intervention Strategies. LID - 10.3390/nu18203350 [doi] LID - 3350 AB - Cold exposure is an important environmental stressor that can disrupt physiological homeostasis and induce complex metabolic, immune, oxidative, and gastrointestinal responses. Increasing evidence indicates that the intestine is a sensitive target of cold exposure, yet the underlying mechanisms and their interrelationships remain incompletely understood. This review summarizes and critically evaluates current evidence on cold exposure-induced intestinal dysfunction and adaptation, with emphasis on intestinal barrier integrity, mucus and digestive function, inflammatory and oxidative responses, pyroptosis-related signaling, gut microbiota remodeling, animal models, and potential intervention strategies. Experimental studies in rodents, pigs, poultry, and fish indicate that cold exposure can induce intestinal morphological alterations, epithelial barrier disruption, increased permeability, tight-junction remodeling, mucus and digestive dysfunction, inflammatory activation, oxidative stress, apoptosis, and, under specific conditions, inflammasome-associated pyroptosis. Concurrent alterations in gut microbial composition and metabolites may interact with epithelial and immune responses, although the temporal and causal relationships among these processes remain insufficiently defined. Importantly, intestinal responses to cold are highly context-dependent and vary according to exposure temperature, duration, pattern, species, nutritional status, acclimation history, and pre-existing disease. Evidence from experimental colitis models further suggests that controlled cold exposure may promote mucosal repair and antimicrobial defense under selected conditions, indicating that cold exposure is not universally detrimental to intestinal health. This review also evaluates nutritional and food-derived strategies, including rutin, plant-derived preparations, dietary glucose, sodium butyrate, vitamin D3, probiotics, prebiotics, and other microbiota-targeted approaches, which have shown potential in mitigating cold-associated intestinal disturbances. Finally, major methodological and translational challenges are discussed, including the need for standardized cold-exposure protocols, causal and longitudinal studies, functional microbiome analysis, and better translation from animal models to human health. Collectively, current evidence supports a multidimensional and context-dependent framework for understanding how cold exposure influences intestinal homeostasis and highlights nutritional and food-derived interventions as promising strategies for maintaining intestinal health under cold environmental conditions. FAU - Guo, Shujie AU - Guo S AD - Food Laboratory of Zhongyuan, Henan University of Technology, Zhengzhou 450001, China. FAU - Geng, Zhanhui AU - Geng Z AD - Academy of Military Sciences, PLA, Systems Engineering Institute, Beijing 100010, China. FAU - Zhang, Jingman AU - Zhang J AD - Food Laboratory of Zhongyuan, Henan University of Technology, Zhengzhou 450001, China. FAU - Dou, Beibei AU - Dou B AD - Food Laboratory of Zhongyuan, Luohe 462000, China. FAU - Liu, Yang AU - Liu Y AD - Food Laboratory of Zhongyuan, Luohe 462000, China. FAU - Wang, Xiaonan AU - Wang X AD - Food Laboratory of Zhongyuan, Luohe 462000, China. FAU - Jiao, Yingze AU - Jiao Y AD - Food Laboratory of Zhongyuan, Luohe 462000, China. FAU - Li, Qianwen AU - Li Q AD - Food Laboratory of Zhongyuan, Luohe 462000, China. FAU - Chen, Han AU - Chen H AUID- ORCID: 0000-0002-6729-722X AD - Food Laboratory of Zhongyuan, China Agricultural University, Beijing 100080, China. LA - eng GR - 2023YFF1104503/the National Key R&D Program of China/ PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 SB - IM MH - Animals MH - *Cold Temperature/adverse effects MH - Intestinal Barrier Function MH - Humans MH - Intestinal Mucosa/metabolism MH - *Intestinal Diseases/etiology/physiopathology MH - Gastrointestinal Microbiome/physiology MH - *Intestines/physiopathology MH - Disease Models, Animal MH - Oxidative Stress PMC - PMC13708026 OTO - NOTNLM OT - animal models OT - cold exposure OT - food-derived bioactive compounds OT - gut microbiota OT - inflammation OT - intestinal barrier OT - nutritional interventions OT - oxidative stress COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/13 CRDT- 2026/10/27 10:43 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - nu18203350 [pii] AID - nutrients-18-03350 [pii] AID - 10.3390/nu18203350 [doi] PST - epublish SO - Nutrients. 2026 Oct 13;18(20):3350. doi: 10.3390/nu18203350. PMID- 42942905 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 12 TI - Nicotinamide Mononucleotide Does Not Prevent Cardiac Hypertrophy but Attenuates Cardiac Stress and Remodeling Markers in Mice Exposed to Intermittent Normobaric Hypoxia. LID - 10.3390/nu18203334 [doi] LID - 3334 AB - Background: Nicotinamide mononucleotide (NMN) is a naturally occurring, biologically active nucleotide that serves as a crucial NAD+ precursor in various physiological conditions. Although intermittent hypoxia is known to substantially induce various health problems, whether NMN protects against injury induced by intermittent normobaric hypoxia remains unclear. Methods: In this study, mice were randomly assigned to groups under normoxia, intermittent normobaric hypoxia, and hypoxia with NMN intervention, and the effects of NMN were evaluated by examining organ indices, hematology, histopathology, and 16S rRNA gene amplicon sequencing. Results: Intermittent normobaric hypoxia significantly increased heart weight, cardiac index, right ventricular weight and index, spleen index, serum lactate dehydrogenase activity, and the cardiac expression of BNP and Myh7, indicating pathological cardiac hypertrophy and activation of a ventricular stress response. It also reduced the beta diversity of the gut microbiota and altered its composition. Although NMN intervention did not significantly affect most organ or hematological indices, except for a further increase in serum LDH activity, it significantly attenuated the hypoxia-induced expression of BNP and Myh7 in the right ventricle, restored the spleen weight and index, and changed the composition of the gut microbiota. These exploratory results indicate that NMN does not prevent intermittent normobaric hypoxia-induced cardiac hypertrophy at the organ level but mitigates cardiac stress markers, ameliorates splenic changes, and modulates the gut microbiota. Conclusions: These findings suggest that NMN produced selective molecular and systemic effects under hypoxic exposure rather than an overall protective phenotype and warrant further investigation. FAU - Wang, Zhen AU - Wang Z AD - Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, China. AD - Capital Institute of Pediatrics, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100020, China. AD - State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China. FAU - Wang, Mingxuan AU - Wang M AD - Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, China. AD - College of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050091, China. FAU - Guo, Jingling AU - Guo J AD - State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China. FAU - Wang, Yajing AU - Wang Y AD - State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China. FAU - Wang, Shijie AU - Wang S AD - College of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050091, China. FAU - Yuan, Jing AU - Yuan J AD - Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, China. AD - Capital Institute of Pediatrics, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100020, China. FAU - Yang, Ruifu AU - Yang R AD - State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China. FAU - Bi, Yujing AU - Bi Y AD - State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China. FAU - Ke, Yuehua AU - Ke Y AUID- ORCID: 0000-0001-6054-880X AD - Department of Bacteriology, Capital Institute of Pediatrics, Beijing 100020, China. AD - Capital Institute of Pediatrics, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100020, China. AD - College of Food Science and Biology, Hebei University of Science and Technology, Shijiazhuang 050091, China. LA - eng GR - 2024-1-1132/Capital Health Development Research Special Project/ GR - L232010/Key Project of Beijing Natural Science Foundation/ GR - JYY2023-19/Beijing Municipal Public Welfare Development and Reform Pilot Project for Medical Research Institutes/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - EC 3.6.4.1 (Myosin Heavy Chains) RN - 1094-61-7 (Nicotinamide Mononucleotide) RN - 0 (Biomarkers) RN - 114471-18-0 (Natriuretic Peptide, Brain) RN - 0 (Myh7 protein, mouse) RN - EC 1.1.1.27 (L-Lactate Dehydrogenase) SB - IM MH - Animals MH - *Hypoxia/complications/physiopathology MH - *Cardiomegaly/prevention & control/etiology MH - Male MH - Myosin Heavy Chains/metabolism/genetics MH - Mice MH - *Nicotinamide Mononucleotide/pharmacology MH - Biomarkers/blood MH - Natriuretic Peptide, Brain/metabolism/genetics MH - Mice, Inbred C57BL MH - *Ventricular Remodeling/drug effects MH - L-Lactate Dehydrogenase/blood PMC - PMC13708252 OTO - NOTNLM OT - BNP OT - Myh7 OT - NAD+ OT - NMN OT - gut microbiota OT - intermittent normobaric hypoxia OT - myocardial hypertrophy COIS- The authors declare no competing interests. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/12 CRDT- 2026/10/27 10:43 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - nu18203334 [pii] AID - nutrients-18-03334 [pii] AID - 10.3390/nu18203334 [doi] PST - epublish SO - Nutrients. 2026 Oct 12;18(20):3334. doi: 10.3390/nu18203334. PMID- 42942900 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 11 TI - The Gut Microbiome-Estrogen Axis in Menopause: Implications for Body Composition, Cardiometabolic Health and Nutrition. LID - 10.3390/nu18203330 [doi] LID - 3330 AB - Background/Objectives: Menopause is associated with profound endocrine, metabolic, immune, and microbial changes that may contribute to accelerated biological aging and increased cardiometabolic risk. The gut microbiome represents a potential interface between estrogen biology, host metabolism, inflammation, and systemic organ function. This narrative review examines the role of menopause-associated gut microbiome remodeling in microbiome-mediated metabolic aging, focusing on the estrobolome, microbial metabolites, organ crosstalk, and microbiome-targeted interventions. Methods: Relevant literature was identified through searches of PubMed/MEDLINE, Scopus, and Web of Science, supplemented by reference-list screening. Human observational and clinical studies, mechanistic investigations, systematic reviews, meta-analyses, and relevant guidelines were critically evaluated and synthesized narratively. No formal meta-analysis was performed. Results: Menopause-associated changes in gut microbial composition and function may influence estrogen metabolism through the estrobolome and modify the production of short-chain fatty acids, lipopolysaccharides, trimethylamine N-oxide, and bile acid metabolites. These pathways may contribute to chronic low-grade inflammation, insulin resistance, endothelial dysfunction, dyslipidemia, hepatic metabolic dysfunction, adipose tissue inflammation, adverse changes in body composition, sarcopenia, neuroinflammation, and urogenital microbial instability. Dietary patterns, fermentable fiber, physical activity, probiotics, synbiotics, phytoestrogens, and menopausal hormone therapy may influence components of this network, although the strength of evidence remains variable. Current data do not support the use of microbiome characteristics to select or dose hormone therapy. Conclusions: The gut microbiome may act as a dynamic mediator linking estrogen decline with metabolic and systemic aging after menopause. However, causality, individual variability, and clinical translation remain insufficiently established. Longitudinal studies integrating shotgun metagenomics, metabolomics, hormone measurements, standardized hormone therapy exposure, and clinical outcomes are needed to determine whether microbiome-derived biomarkers can support risk stratification and personalized interventions for healthier aging in postmenopausal women. FAU - Patru, Oana AU - Patru O AUID- ORCID: 0009-0006-6510-5921 AD - Cardiology Department, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timisoara, Romania. AD - Research Center of the Institute of Cardiovascular Diseases Timisoara, 13A Gheorghe Adam Street, 300310 Timisoara, Romania. AD - Doctoral School, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timisoara, Romania. AD - Institute of Cardiovascular Diseases Timisoara, 13A Gheorghe Adam Street, 300310 Timisoara, Romania. FAU - Luca, Silvia AU - Luca S AUID- ORCID: 0009-0005-0859-2498 AD - Cardiology Department, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timisoara, Romania. AD - Research Center of the Institute of Cardiovascular Diseases Timisoara, 13A Gheorghe Adam Street, 300310 Timisoara, Romania. AD - Doctoral School, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timisoara, Romania. AD - Institute of Cardiovascular Diseases Timisoara, 13A Gheorghe Adam Street, 300310 Timisoara, Romania. FAU - Paunescu, Andrei AU - Paunescu A AD - Department of Urology, "Pius Brinzeu" Emergency Clinical County University Hospital, 300723 Timisoara, Romania. FAU - Enache, Bogdan AU - Enache B AUID- ORCID: 0000-0003-2806-9002 AD - Cardiology Department, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timisoara, Romania. AD - Research Center of the Institute of Cardiovascular Diseases Timisoara, 13A Gheorghe Adam Street, 300310 Timisoara, Romania. AD - Institute of Cardiovascular Diseases Timisoara, 13A Gheorghe Adam Street, 300310 Timisoara, Romania. FAU - Vacarescu, Cristina AU - Vacarescu C AD - Cardiology Department, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timisoara, Romania. AD - Research Center of the Institute of Cardiovascular Diseases Timisoara, 13A Gheorghe Adam Street, 300310 Timisoara, Romania. AD - Institute of Cardiovascular Diseases Timisoara, 13A Gheorghe Adam Street, 300310 Timisoara, Romania. FAU - Cozma, Dragos AU - Cozma D AUID- ORCID: 0009-0008-7378-7787 AD - Cardiology Department, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timisoara, Romania. AD - Research Center of the Institute of Cardiovascular Diseases Timisoara, 13A Gheorghe Adam Street, 300310 Timisoara, Romania. AD - Institute of Cardiovascular Diseases Timisoara, 13A Gheorghe Adam Street, 300310 Timisoara, Romania. FAU - Crisan, Simina AU - Crisan S AUID- ORCID: 0000-0002-8760-5605 AD - Cardiology Department, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timisoara, Romania. AD - Research Center of the Institute of Cardiovascular Diseases Timisoara, 13A Gheorghe Adam Street, 300310 Timisoara, Romania. AD - Institute of Cardiovascular Diseases Timisoara, 13A Gheorghe Adam Street, 300310 Timisoara, Romania. FAU - Bena, Andreea AU - Bena A AD - Discipline of Endocrinology, Second Department of Internal Medicine, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timisoara, Romania. AD - Center for Molecular Research in Nephrology and Vascular Disease, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timisoara, Romania. LA - eng PT - Journal Article PT - Review DEP - 20261011 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Estrogens) SB - IM MH - Humans MH - *Menopause/physiology/metabolism MH - Female MH - *Estrogens/metabolism MH - *Gastrointestinal Microbiome/physiology MH - *Body Composition/physiology MH - *Nutritional Status MH - Aging MH - Cardiometabolic Risk Factors PMC - PMC13708158 OTO - NOTNLM OT - body composition OT - cardiometabolic health OT - estrobolome OT - estrogen metabolism OT - gut microbiome OT - menopause OT - postmenopausal women OT - precision nutrition OT - women's health COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/11 CRDT- 2026/10/27 10:43 PHST- 2026/09/13 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - nu18203330 [pii] AID - nutrients-18-03330 [pii] AID - 10.3390/nu18203330 [doi] PST - epublish SO - Nutrients. 2026 Oct 11;18(20):3330. doi: 10.3390/nu18203330. PMID- 42942895 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 10 TI - FoodAllergenSensitization in a Five-Year Allergy Clinic Cohort and Food Allergy Knowledge, Attitudes and Behaviours in Romanian Adults: Two Observational Studies. LID - 10.3390/nu18203324 [doi] LID - 3324 AB - Background/Objectives: Food allergy is reported far more often than it is confirmed by testing. Both ends of that gap matter nutritionally: dietary restriction adopted without confirmation, and unpreparedness for severe reactions. We characterized each end in two cohorts from one source population. Methods: Study 1 retrospectively analyzed 4404 patients assessed in a single allergy service over 2020-2024, with food sensitization being a positive skin prick test and/or specific IgE to at least one food allergen. Study 2 surveyed 500 adults by convenience sampling, using a 27-item questionnaire with a 10-point knowledge score. In Study 1, testing was indication-driven, and the food-tested subset could not be identified, so all Study 1 figures are proportions among patients assessed, not prevalence estimates. Analyses reported effect sizes with confidence intervals throughout, applied Holm-Bonferroni adjustment to the confirmatory comparisons, and used multivariable regression. Results: Food sensitization was documented in 185 of 4404 patients (4.2%; 95% CI 3.6-4.8) and concentrated in early childhood (13.05% at 0-5 years), age being the only independent demographic predictor (adjusted OR for 0-5 vs. 18-30 years: 4.61; 95% CI 2.87-7.41)-an association confounded with the indication to test. Of food-sensitized patients with a recorded severity score, one third (34.1%) had no active symptoms at assessment. In the community cohort, 17.6% (95% CI 14.5-21.2) self-reported a food allergy, but only 6.6% (95% CI 4.7-9.1) reported one as test-confirmed-a gap of 11.0 percentage points (95% CI 8.5-14.0). The mean knowledge score was 5.08/10: 70.2% knew anaphylaxis can be fatal, yet only 47.8% named adrenaline as first-line treatment, 33.4% had never heard of an auto-injector, and 15.4% always read allergen labels. Educational level and health-sector training were the strongest predictors of knowledge. Conclusions: Food allergy is labelled more readily than it is verified, while preparedness for severe reactions is thin. Better access to diagnostic confirmation and public information stratified by educational attainment are needed. The two cohorts share a source population but are differently selected and measure different constructs, so their proportions are complementary rather than interchangeable. FAU - Iovin, Valentin-Cristian AU - Iovin VC AUID- ORCID: 0009-0004-1552-7961 AD - Doctoral School Department, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. FAU - Lighezan, Diana AU - Lighezan D AUID- ORCID: 0009-0002-8001-7672 AD - Department of Hematology, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. FAU - Neamtu, Andreea-Adriana AU - Neamtu AA AUID- ORCID: 0000-0002-5219-5466 AD - Department of Toxicology, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. AD - Research Centre for Pharmaco-Toxicological Evaluation, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. AD - Department of Pathology, Clinical County Emergency Hospital of Arad, 310037 Arad, Romania. AD - Department of Pathology, "Pius Brinzeu" Clinical County Emergency Hospital, 300723 Timisoara, Romania. FAU - Porr, Corina AU - Porr C AUID- ORCID: 0009-0000-7255-0835 AD - Allergology Department, Faculty of Medicine, "Lucian Blaga" University, 550169 Sibiu, Romania. FAU - Capraru, Anca-Maria AU - Capraru AM AUID- ORCID: 0009-0001-5908-0231 AD - Doctoral School, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. AD - Department of Pharmacy, Poiana Mare Psychiatry Hospital, Garii Str. No. 40, 207470 Poiana Mare, Romania. FAU - Sarau, Cristian AU - Sarau C AUID- ORCID: 0000-0002-1938-1443 AD - Medical Semiology I, Department V Internal Medicine I, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. FAU - Juganaru, Iulius AU - Juganaru I AUID- ORCID: 0009-0000-6879-145X AD - Department XI Pediatrics, Discipline I Pediatrics, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. AD - Department of Pediatrics I, "Louis Turcanu" Children's Emergency Hospital, 300011 Timisoara, Romania. FAU - Muntean, Adriana AU - Muntean A AUID- ORCID: 0000-0002-1724-5915 AD - Department of Allergology and Clinical Immunology, "Iuliu Hatieganu" University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. FAU - Bugi, Meda-Ada AU - Bugi MA AUID- ORCID: 0009-0000-2470-8706 AD - Department of Pediatrics I, "Louis Turcanu" Children's Emergency Hospital, 300011 Timisoara, Romania. AD - Department of Pharmacy, University of Medicine and Pharmacy "Vasile Goldis", 310025 Arad, Romania. FAU - Panaitescu, Carmen AU - Panaitescu C AUID- ORCID: 0000-0001-8749-9972 AD - Department III Functional Sciences, Physiology Discipline, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. AD - Centre of Immuno-Physiology and Biotechnologies (CIFBIOTEH), "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. AD - OncoGen Centre, "Pius Brinzeu" Clinical County Emergency Hospital, 300723 Timisoara, Romania. LA - eng GR - Victor Babes University of Medicine and Pharmacy Timisoara/ PT - Journal Article PT - Observational Study DEP - 20261010 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Allergens) RN - 37341-29-0 (Immunoglobulin E) SB - IM MH - Humans MH - *Food Hypersensitivity/epidemiology/diagnosis/immunology MH - Adult MH - Female MH - Male MH - *Health Knowledge, Attitudes, Practice MH - Retrospective Studies MH - Adolescent MH - Young Adult MH - Middle Aged MH - Romania/epidemiology MH - Skin Tests MH - *Allergens/immunology MH - Surveys and Questionnaires MH - Immunoglobulin E/blood MH - Infant MH - Child, Preschool MH - Prevalence MH - Child PMC - PMC13708197 OTO - NOTNLM OT - Romania OT - adrenaline auto-injector OT - allergen labelling OT - anaphylaxis OT - cross-sectional survey OT - elimination diet OT - food allergy OT - food sensitization OT - health literacy OT - skin prick test COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/10 CRDT- 2026/10/27 10:43 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - nu18203324 [pii] AID - nutrients-18-03324 [pii] AID - 10.3390/nu18203324 [doi] PST - epublish SO - Nutrients. 2026 Oct 10;18(20):3324. doi: 10.3390/nu18203324. PMID- 42942889 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 10 TI - Natural Products in Diabetes Mellitus: A Narrative Review of Mechanistic Insights into Metabolic Regulation and Therapeutic Potential. LID - 10.3390/nu18203318 [doi] LID - 3318 AB - Diabetes mellitus is a complex metabolic disease characterized by persistent hyperglycemia resulting from impaired insulin secretion, insulin action, or both. In addition to disturbances in glucose metabolism, the disease is accompanied by abnormalities in mitochondrial dysfunction, oxidative stress, lipid and protein metabolism, and chronic inflammation that contribute to progressive tissue injury and the development of vascular complications. Although current pharmacological therapies effectively improve glycemic control, many patients continue to experience disease progression and diabetes-associated complications, highlighting the need for complementary therapeutic strategies that target multiple pathogenic mechanisms. Growing evidence suggests that naturally occurring bioactive compounds may provide such multitarget benefits. Plant-derived polyphenols, medicinal herbs, culinary spices, and functional foods have demonstrated antioxidant, anti-inflammatory, hypoglycemic, hypolipidemic, and insulin-sensitizing properties in experimental and clinical studies. Compounds including curcumin, honey, garlic, fenugreek, cumin, shilajit, and guduchi have been reported to influence several molecular pathways involved in diabetes pathogenesis, including NF-kappaB-mediated inflammatory signaling, oxidative and endoplasmic reticulum stress, PPARgamma activation, advanced glycation end-product formation, and pancreatic beta-cell preservation. This review critically examines current evidence regarding the role of natural products in diabetes management, integrating findings from cell-based studies, animal models, and human clinical investigations. Particular emphasis is placed on the interplay between oxidative stress, inflammation, mitochondrial dysfunction, and metabolic regulation, as these interconnected processes represent key drivers of disease progression. In addition to summarizing established mechanisms, the review discusses current limitations, translational challenges, and future research priorities. A deeper understanding of how natural products modulate these pathways may facilitate the development of evidence-based adjunctive strategies to improve long-term metabolic health and reduce the burden of diabetic complications. This narrative review critically synthesizes published peer-reviewed mechanistic, preclinical, and clinical evidence regarding selected natural products and functional foods relevant to diabetes mellitus. FAU - Deshmukh, Hemalata AU - Deshmukh H AD - Department of Biological Sciences, Texas Tech University, 2500 Broadway, Lubbock, TX 79409, USA. FAU - Kshirsagar, Ganesh J AU - Kshirsagar GJ AD - Department of Biological Sciences, Texas Tech University, 2500 Broadway, Lubbock, TX 79409, USA. FAU - Gollahon, Lauren AU - Gollahon L AUID- ORCID: 0000-0002-1280-3359 AD - Department of Biological Sciences, Texas Tech University, 2500 Broadway, Lubbock, TX 79409, USA. LA - eng PT - Journal Article PT - Review DEP - 20261010 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Biological Products) RN - 0 (Hypoglycemic Agents) SB - IM MH - Humans MH - *Biological Products/therapeutic use/pharmacology MH - Animals MH - *Diabetes Mellitus/drug therapy/metabolism MH - Oxidative Stress/drug effects MH - *Hypoglycemic Agents/therapeutic use/pharmacology PMC - PMC13708169 OTO - NOTNLM OT - diabetes mellitus OT - inflammation OT - insulin resistance OT - metabolic regulation OT - mitochondrial dysfunction OT - natural products OT - oxidative stress COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/10 CRDT- 2026/10/27 10:43 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - nu18203318 [pii] AID - nutrients-18-03318 [pii] AID - 10.3390/nu18203318 [doi] PST - epublish SO - Nutrients. 2026 Oct 10;18(20):3318. doi: 10.3390/nu18203318. PMID- 42942886 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 9 TI - Potential Role of Oral Nitrate Bioactivation in Interindividual Exercise Responses to Dietary Nitrate: A Narrative Review. LID - 10.3390/nu18203315 [doi] LID - 3315 AB - Background: Equivalent doses of dietary nitrate often yield highly heterogeneous exercise responses across individuals. Efficient enterosalivary nitrate-to-nitrite conversion is dependent on oral commensal bacteria, representing a critical upstream step that may drive downstream variability in biomarker profiles and exercise outcomes. This review evaluates whether variation at the oral bioactivation step helps account for differences in downstream biomarkers and exercise performance. Methods: We conducted a structured narrative review of PubMed and Web of Science covering 1 January 2006 to 31 March 2026 using predefined keywords related to dietary nitrate, oral bioactivation, oral microbiota, enterosalivary pathway, and exercise performance. Human supplementation trials, antibacterial mouthwash perturbation studies, investigations evaluating oral function, and supporting case-report and preclinical evidence were included when they linked nitrate exposure, oral microbial activity or salivary nitrite kinetics, and physiological or exercise performance outcomes. Results: Oral bacteria are essential contributors to efficient enterosalivary nitrate-to-nitrite conversion. Associations between oral function and nitrite availability, as well as selected physiological measures, are reasonably consistent. However, direct evidence linking oral function to athlete performance remains sparse. No athlete trial identified in the present search has concurrently measured oral function, tracked serial nitrate/nitrite kinetics, and paired these data with a reliable performance test and prespecified mediation analysis. A rise in salivary nitrite therefore confirms engagement of the enterosalivary pathway, but it does not reliably identify an ergogenic responder. Current evidence does not yet allow quantitative estimation of how much between-person performance variability is attributable to oral bioactivation. Conclusions: Oral nitrate-reducing function remains a biologically plausible modifier of exercise responses, but it is not a validated predictive biomarker. Integrated athlete trials are needed to trace the full pathway from nitrate exposure to performance outcomes and to quantify within-person and between-person variation. FAU - Zhao, Zhengxin AU - Zhao Z AD - Department of Sports, Nanjing University of Finance and Economics HongShan College, Nanjing 211300, China. FAU - Lai, Zheng AU - Lai Z AD - Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. FAU - Zhao, Shengwei AU - Zhao S AD - Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. FAU - Li, Wenli AU - Li W AD - Anhui Provincial Key Laboratory of Zoonoses, Department of Microbiology and Parasitology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China. FAU - Wang, Min AU - Wang M AD - Department of Sports, Nanjing University of Finance and Economics HongShan College, Nanjing 211300, China. FAU - Guan, Feng AU - Guan F AD - Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. FAU - Liang, Xu AU - Liang X AD - Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. FAU - Zhang, Jiawei AU - Zhang J AUID- ORCID: 0000-0001-5004-3402 AD - Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. FAU - Wang, Linding AU - Wang L AUID- ORCID: 0000-0001-6877-5791 AD - Anhui Provincial Key Laboratory of Zoonoses, Department of Microbiology and Parasitology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China. LA - eng PT - Journal Article PT - Review DEP - 20261009 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Nitrates) RN - 0 (Nitrites) RN - 0 (Biomarkers) SB - IM MH - Humans MH - *Nitrates/administration & dosage/metabolism/pharmacokinetics MH - *Exercise/physiology MH - Nitrites/metabolism MH - *Mouth/microbiology MH - Saliva/chemistry/metabolism MH - *Athletic Performance/physiology MH - *Diet MH - Dietary Supplements MH - Microbiota MH - Biomarkers PMC - PMC13708611 OTO - NOTNLM OT - dietary nitrate OT - exercise performance OT - interindividual variability OT - nitrate-reducing bacteria OT - nitric oxide OT - oral microbiome OT - oral nitrate bioactivation OT - salivary nitrite OT - sports nutrition COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/09 CRDT- 2026/10/27 10:43 PHST- 2026/08/16 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - nu18203315 [pii] AID - nutrients-18-03315 [pii] AID - 10.3390/nu18203315 [doi] PST - epublish SO - Nutrients. 2026 Oct 9;18(20):3315. doi: 10.3390/nu18203315. PMID- 42942883 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 2072-6643 (Electronic) IS - 2072-6643 (Linking) VI - 18 IP - 20 DP - 2026 Oct 9 TI - Impact of GLP-1 Receptor Agonists on Cognitive Performance in Adults at Risk of Alzheimer's Disease: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. LID - 10.3390/nu18203312 [doi] LID - 3312 AB - Background/Objectives: Type 2 diabetes, a widely recognized risk factor of Alzheimer's disease (AD), is mainly due to the role played by insulin resistance on neuronal degeneration. Mild Cognitive Impairment (MCI) also significantly increases the risk of developing AD by approximately 40%. Epidemiological studies have demonstrated correlations between glucagon-like peptide-1 agonists (GLP-1RAs) on reducing the risk of AD. We synthesize current data from randomized controlled trials (RCTs) to summarize the effects of GLP-1RA treatment in adults with Type 2 diabetes or with MCI on cognitive performance. Methods: We searched databases from inception to 1 January 2026 investigating effects of GLP-1RA(s) on cognition. PubMed, Web of Science and Scopus databases were explored following Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). We selected only RCTs in adults investigating GLP-1RA(s) on cognitive performance. Results: Overall mean age was 64.5 yrs with an average follow-up period of 28.4 months. A total of 13,711 participants from five RCTs were included. Following a source-data audit, four randomized controlled trials contributed analytically compatible data to at least one cognitive domain. Cognitive domains were analyzed separately using Hedges' g. Random-effects models used restricted maximum likelihood (REML) estimation with Knapp-Hartung inference when at least two compatible studies contributed. For global cognition, three studies yielded g = 0.014 (95% CI: -0.061 to 0.088; p = 0.515; I(2) = 0%). For attention, two studies yielded g = 0.072 (95% CI: -2.164 to 2.308; p = 0.753; I(2) = 31.1%). For delayed memory, executive function, and verbal function, only one compatible study was available per domain; these were therefore reported as single-study estimates rather than pooled effects. A sensitivity analysis excluding the dominant Cukierman trial yielded an imprecise and non-significant effect estimate for global cognition (g = -0.205, 95% CI: -2.678 to 2.269) indicating that the robustness of the pooled result could not be established. Conclusions: Current randomized evidence is insufficient to establish a consistent beneficial effect of GLP-1RA(s) on cognitive function in adults at risk of AD. The available findings should be considered exploratory and hypothesis-generating because few analytically compatible trials were available within each cognitive domain. FAU - Abbatecola, Angela M AU - Abbatecola AM AUID- ORCID: 0000-0001-5522-9257 AD - Department of Life Sciences, Health, and Health Professions, Link Campus University, Via del Casale Pio V, 00165 Rome, Italy. FAU - Migliara, Giuseppe AU - Migliara G AUID- ORCID: 0000-0002-4573-6025 AD - Department of Life Sciences, Health, and Health Professions, Link Campus University, Via del Casale Pio V, 00165 Rome, Italy. FAU - D'Agostino, Fausto AU - D'Agostino F AD - Department of Anaesthesia, Intensive Care and Pain Management, Campus Bio Medico University and Teaching Hospital, 00128 Rome, Italy. FAU - Veronese, Nicola AU - Veronese N AUID- ORCID: 0000-0002-9328-289X AD - Faculty of Medicine, Saint Camillus International University of Health Sciences, 00131 Rome, Italy. FAU - Giuliani, Angelica AU - Giuliani A AUID- ORCID: 0000-0002-8477-8519 AD - Clinic of Laboratory and Precision Medicine, IRCCS INRCA, 60121 Ancona, Italy. AD - Department of Clinical and Molecular Sciences, Universita Politecnica delle Marche, 60100 Ancona, Italy. FAU - Caponnetto, Salvatore AU - Caponnetto S AUID- ORCID: 0000-0003-2906-449X AD - Department of Life Sciences, Health, and Health Professions, Link Campus University, Via del Casale Pio V, 00165 Rome, Italy. FAU - Corbi, Graziamaria AU - Corbi G AUID- ORCID: 0000-0002-3441-889X AD - Department of Medical and Translational Medicine, University of Naples "Federico II", 80131 Naples, Italy. FAU - Signoriello, Elisabetta AU - Signoriello E AUID- ORCID: 0000-0001-5753-6752 AD - Department of Advanced Medical and Surgical Sciences, University of Campania Luigi Vanvitelli, 80138 Naples, Italy. FAU - Incalzi, Raffaele Antonelli AU - Incalzi RA AD - Research Unit of Geriatrics, Universita Campus Bio-Medico di Rome, 00128 Rome, Italy. AD - Operative Research Unit of Internal Medicine, Fondazione Policlinico Universitario Campus Bio-Medico, 00128 Rome, Italy. FAU - Paolisso, Giuseppe AU - Paolisso G AUID- ORCID: 0000-0002-2137-455X AD - Faculty of Medicine, Saint Camillus International University of Health Sciences, 00131 Rome, Italy. AD - Department of Advanced Medical and Surgical Sciences, University of Campania Luigi Vanvitelli, 80138 Naples, Italy. LA - eng PT - Journal Article PT - Meta-Analysis PT - Review PT - Systematic Review DEP - 20261009 PL - Switzerland TA - Nutrients JT - Nutrients JID - 101521595 RN - 0 (Glucagon-Like Peptide-1 Receptor Agonists) SB - IM MH - Aged MH - Female MH - Humans MH - Male MH - Middle Aged MH - *Alzheimer Disease/prevention & control/etiology MH - *Cognition/drug effects MH - *Cognitive Dysfunction/drug therapy MH - *Diabetes Mellitus, Type 2/drug therapy/complications MH - *Glucagon-Like Peptide-1 Receptor Agonists/pharmacology/therapeutic use MH - Randomized Controlled Trials as Topic MH - Risk Factors PMC - PMC13708722 OTO - NOTNLM OT - RCTs OT - T2DM OT - glucagon-like peptide-1 agonists OT - memory OT - mild cognitive impairment OT - nutrition COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/09 CRDT- 2026/10/27 10:43 PHST- 2026/08/17 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - nu18203312 [pii] AID - nutrients-18-03312 [pii] AID - 10.3390/nu18203312 [doi] PST - epublish SO - Nutrients. 2026 Oct 9;18(20):3312. doi: 10.3390/nu18203312. PMID- 42942870 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 21 TI - Population Pharmacokinetic Models of Voriconazole in Pediatric Patients: A Systematic Review. LID - 10.3390/pharmaceutics18101341 [doi] LID - 1341 AB - Background/Objectives: Voriconazole dosing in children is complicated by developmental and nonlinear pharmacokinetics, cytochrome P450 2C19 (CYP2C19) variation, variable oral bioavailability, inflammation, organ dysfunction, and drug interactions. We evaluated the characteristics, reporting completeness, and clinical applicability of pediatric and pediatric-inclusive voriconazole population pharmacokinetic (PopPK) models. Methods: PubMed, EMBASE, the Cochrane Library, Web of Science, and supplementary sources were searched from inception to 12 March 2026. Eligible studies developed a voriconazole PopPK model in patients aged 0-18 years or in a broader population with extractable or interpretable pediatric information. Reporting completeness was assessed using the 32-item Clinical Pharmacokinetic (ClinPK) checklist. Results: Eighteen studies published from 2004 to 2025 were included. Model-development cohorts contained 21-173 participants, and 15 studies reported 108-3336 concentrations. The median ClinPK reporting-completeness score was 82.6% (range, 74.2-90.0%). The included studies comprised one infant-specific, nine pediatric-only, six pediatric-young adult, and two mixed pediatric-adult models. Fifteen studies used parametric nonlinear mixed-effects methods and three used nonparametric methods; 15 selected two-compartment models and three selected one-compartment models. Body size was incorporated in 17 models and CYP2C19 phenotype was the most frequent retained covariate (10 models). Elimination structures, exposure targets, and dosing proposals were heterogeneous, and only one model underwent independent external validation. Conclusions: A population-matched model may support initial dosing, but early therapeutic drug monitoring and iterative Bayesian updating remain necessary. Current evidence does not support a universal pediatric model. Independent external validation and prospective implementation studies are required before routine clinical use. FAU - Wang, Xinyi AU - Wang X AUID- ORCID: 0009-0004-9279-5732 AD - Department of Clinical Pharmacy, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China. FAU - Li, Min AU - Li M AD - Department of Clinical Pharmacy, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China. FAU - Yuan, Xiyue AU - Yuan X AD - Department of Clinical Pharmacy, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China. FAU - Chen, Jihui AU - Chen J AUID- ORCID: 0000-0002-9819-9921 AD - Department of Clinical Pharmacy, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, China. LA - eng GR - SHWSRS(2026)_071/Shanghai "Rising Stars of Medical Talents" Youth Development Program-Youth Medical Talents-Clinical Pharmacist Program/ PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708257 OTO - NOTNLM OT - model-informed precision dosing OT - pediatrics OT - population pharmacokinetics OT - systematic review OT - therapeutic drug monitoring OT - voriconazole COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:43 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - pharmaceutics18101341 [pii] AID - pharmaceutics-18-01341 [pii] AID - 10.3390/pharmaceutics18101341 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 21;18(10):1341. doi: 10.3390/pharmaceutics18101341. PMID- 42942868 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 21 TI - Oseltamivir-Based Organic Salts and Ionic Liquids: Physicochemical Properties, Cytotoxicity, and Neuraminidase Inhibition. LID - 10.3390/pharmaceutics18101340 [doi] LID - 1340 AB - Background: Neuraminidase (NA) inhibitors are widely used for the treatment of influenza, but the emergence of resistance and limitations in drug formulation can compromise their therapeutic performance. This study investigated whether counterion exchange can modulate the physicochemical properties, cytotoxicity, and NA-inhibitory activity of oseltamivir (OS) while preserving its covalent structure. Methods: Five OS-based ionic formulations, [OS][C(1)SO(3)], [OS][C(3)SO(3)], [OS][C(6)SO(3)], [OS][TolSO(3)], and [OS][CSA], were synthesized and characterized by NMR and ATR-FTIR spectroscopy, with isolated yields ranging from 84 to 88%. Spectroscopic data confirmed the formation of the desired 1:1 salts and showed that the OS ethyl ester remained chemically intact. The formulations were evaluated for aqueous solubility, octanol/water partitioning, cytotoxicity toward MDCK-SIAT1 cells, and NA-inhibitory activity using a MUNANA-based assay against NAs from A(H1N1)pdm09, its H275Y oseltamivir-resistant variant, A(H3N2), and type B influenza viruses. Results: All formulations exhibited low cytotoxicity, with CC(50) values above 1200 microM. All OS ionic formulations showed lower IC(50) values than oseltamivir phosphate against all four influenza viruses, with improvements ranging from approximately 2.5- to 393-fold. [OS][TolSO(3)] showed the highest potency, reducing the IC(50) by approximately 336-fold against H275Y virus NA and 393-fold against influenza B virus NA. IC(90) values showed the same overall potency ranking, although improvements at 50% inhibition were not always proportionally maintained at 90% inhibition. Conclusions: Overall, counterion selection substantially influenced both the physicochemical and biological profiles of OS. [OS][TolSO(3)] provided the highest NA-inhibitory potency, whereas [OS][CSA] exhibited the most balanced overall profile, supporting counterion engineering as a potential approach to optimize oseltamivir-based ionic formulations. FAU - Madeira, Diogo A AU - Madeira DA AUID- ORCID: 0000-0003-3021-9815 AD - LAQV-REQUIMTE, Chemistry Department, NOVA School of Science and Technology (NOVA FCT), 2829-516 Caparica, Portugal. FAU - Correia, Vanessa AU - Correia V AUID- ORCID: 0000-0002-1619-1900 AD - Antiviral Resistance Lab, Research & Development Unit, Infectious Diseases Department, Instituto Nacional de Saude Doutor Ricardo Jorge, IP, Av. Padre Cruz, 1649-016 Lisboa, Portugal. AD - Host-Pathogen Interaction Unit, Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Professor Gama Pinto, 1649-003 Lisboa, Portugal. FAU - Santos, Luis AU - Santos L AUID- ORCID: 0000-0001-5915-1186 AD - Antiviral Resistance Lab, Research & Development Unit, Infectious Diseases Department, Instituto Nacional de Saude Doutor Ricardo Jorge, IP, Av. Padre Cruz, 1649-016 Lisboa, Portugal. AD - Host-Pathogen Interaction Unit, Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Professor Gama Pinto, 1649-003 Lisboa, Portugal. FAU - Rebelo-de-Andrade, Helena AU - Rebelo-de-Andrade H AUID- ORCID: 0000-0002-0138-0944 AD - Antiviral Resistance Lab, Research & Development Unit, Infectious Diseases Department, Instituto Nacional de Saude Doutor Ricardo Jorge, IP, Av. Padre Cruz, 1649-016 Lisboa, Portugal. AD - Host-Pathogen Interaction Unit, Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Professor Gama Pinto, 1649-003 Lisboa, Portugal. FAU - Branco, Luis C AU - Branco LC AUID- ORCID: 0000-0003-2520-1151 AD - LAQV-REQUIMTE, Chemistry Department, NOVA School of Science and Technology (NOVA FCT), 2829-516 Caparica, Portugal. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708149 OTO - NOTNLM OT - active pharmaceutical ingredient OT - antiviral resistance OT - influenza virus OT - ionic liquids OT - neuraminidase inhibitor OT - organic salts OT - oseltamivir COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:43 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - pharmaceutics18101340 [pii] AID - pharmaceutics-18-01340 [pii] AID - 10.3390/pharmaceutics18101340 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 21;18(10):1340. doi: 10.3390/pharmaceutics18101340. PMID- 42942864 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 21 TI - Physiologically Based Pharmacokinetic Modeling of Venetoclax in Pediatric Acute Myeloid Leukemia: Food Effects, Age-Related Pharmacokinetics, and Voriconazole-Mediated Drug-Drug Interactions. LID - 10.3390/pharmaceutics18101335 [doi] LID - 1335 AB - Background: Venetoclax (VEN), a selective B-cell lymphoma 2 inhibitor, has been increasingly used off-label in pediatric patients with hematologic malignancies. However, its pharmacokinetic characteristics in pediatric patients remain insufficiently characterized. Methods: An adult VEN physiologically based pharmacokinetic (PBPK) model was developed and extrapolated to children using age-dependent physiological and CYP3A4 ontogeny data. The pediatric model was calibrated and evaluated using data from 76 children with AML (203 plasma concentrations) and subsequently applied to assess food effects, age-related pharmacokinetic differences, and VEN dose-reduction scenarios during VCZ co-administration. The clinical study was registered with the Chinese Clinical Trial Registry (ChiCTR1900027146 and ChiCTR2400080307). Results: The adult and pediatric models adequately reproduced the observed VEN concentration-time profiles. At 200 mg/m(2), a normal-fat meal increased predicted AUC(inf) and C(max) by 2.74- and 1.77-fold, respectively, versus a low-fat meal. Children aged 2-<6 years had the highest exposure; AUC(inf) was 1.44- and 1.91-fold higher than in the 6-<12- and 12-<18-year groups, respectively. During VCZ co-administration, reducing VEN to 50% and 25% of the full dose decreased AUC(0-168h) by 36.8% and 60.5% and C(max) by 42.5% and 70.5%, respectively, with minimal changes in T(max). Conclusions: The pediatric VEN PBPK model quantitatively characterized the effects of meal conditions, developmental age, and VCZ-mediated CYP3A inhibition on VEN pharmacokinetics. These findings support administration with a normal diet, closer safety monitoring in children aged 2-<6 years receiving 200 mg/m(2), and model-informed evaluation of VEN dose reduction during concomitant VCZ therapy. FAU - Li, Yanming AU - Li Y AUID- ORCID: 0000-0001-8605-682X AD - Department of Pharmacy, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China. FAU - Liu, Mengmeng AU - Liu M AUID- ORCID: 0000-0002-9872-4212 AD - Department of Pharmacy, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China. AD - Department of Pharmacy, Peking University Third Hospital, Beijing 100191, China. FAU - Ling, Qixian AU - Ling Q AD - Department of Pharmacy, Peking University Third Hospital, Beijing 100191, China. FAU - Sun, Ning AU - Sun N AD - Department of Pharmacy, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China. FAU - Zheng, Huyong AU - Zheng H AD - Department of Leukemia, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China. FAU - Guo, Peng AU - Guo P AD - Department of Pharmacy, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China. FAU - Zhao, Libo AU - Zhao L AUID- ORCID: 0000-0002-4968-6045 AD - Department of Pharmacy, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China. AD - Department of Pharmacy, Peking University Third Hospital, Beijing 100191, China. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708470 OTO - NOTNLM OT - Chinese population OT - PBPK modeling OT - acute myeloid leukemia OT - pediatric population OT - venetoclax COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:43 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - pharmaceutics18101335 [pii] AID - pharmaceutics-18-01335 [pii] AID - 10.3390/pharmaceutics18101335 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 21;18(10):1335. doi: 10.3390/pharmaceutics18101335. PMID- 42942860 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 20 TI - Pharmaceutical 3D Printing Beyond Printability: Polymer Functions, Structural Design, and Predictable Drug Release. LID - 10.3390/pharmaceutics18101330 [doi] LID - 1330 AB - Oral solid dosage forms, due to their mature manufacturing processes and stability, have become the cornerstone of clinical drug therapy. However, fixed dosages, uniform components, and large-scale production may not meet increasingly complex personalized requirements. The emergence of 3D-printed drugs provides a potential solution to this challenge. Drug 3D printing has progressed beyond feasibility verification and is moving toward functional dosage form design. Current research primarily focuses on printability; however, this review integrates printing technology, polymer functionality, structural design, release behavior, and translational requirements, summarizing mainstream printing technologies, including Fused Deposition Modeling (FDM), Direct Ink Writing/Semi-Solid Extrusion (DIW/SSE), Powder Bed Printing (SLS/BJ), and Photopolymerization (SLA/DLP). Polymers are classified according to drug release control functions, illustrating how modulation of porosity, filling density, shell thickness, material partitioning, and spatial drug distribution enables 3D-printed dosage forms with diverse therapeutic functions. These functions include immediate and sustained release, gastric retention, intestinal targeting, and sequential release of multiple components. This review provides guidance for technical selection, structural design, and formulation development of 3D-printed drugs, while highlighting major translational barriers, including limited printable excipients, empirical formulation development, insufficient structure-based release prediction, quality control challenges, and weak clinical evidence. Future progress requires close integration of material, process, structure, and performance, enabling 3D-printed drugs to evolve from individual prototypes into scalable, quality-controllable, and clinically translatable drug delivery systems. FAU - Liu, Zhiting AU - Liu Z AD - School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China. AD - State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau 999078, China. FAU - Huang, Yi AU - Huang Y AD - Guangdong Provincial Key Laboratory of Pharmaceutical Preparations Research and Evaluation & Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China. FAU - Liu, Yanpai AU - Liu Y AD - Key Specialty of Clinical Pharmacy, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, China. FAU - Huang, Rui AU - Huang R AD - Guangdong Pharmaceutical University, Guangzhou 510006, China. FAU - Xie, Peixuan AU - Xie P AD - Guangdong Pharmaceutical University, Guangzhou 510006, China. FAU - Ma, Xinyao AU - Ma X AD - Guangdong Pharmaceutical University, Guangzhou 510006, China. FAU - Chen, Peihong AU - Chen P AUID- ORCID: 0000-0003-1094-1560 AD - School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou 510006, China. FAU - Li, Xiaofang AU - Li X AD - Guangdong Provincial Key Laboratory of Pharmaceutical Preparations Research and Evaluation & Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China. LA - eng GR - 202610573006/2026 National Innovation and Entrepreneurship Training Program for College Students/ GR - S202610573015/the 2026 Provincial Innovation and Entrepreneurship Training Program for College Students/ GR - S202610573002S/the 2026 Provincial Innovation and Entrepreneurship Training Program for College Students/ PT - Journal Article PT - Review DEP - 20261020 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708645 OTO - NOTNLM OT - 3D printing OT - 3D-printed medicine OT - additive manufacturing OT - oral solid dosage forms OT - polymer OT - quality by design COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:43 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - pharmaceutics18101330 [pii] AID - pharmaceutics-18-01330 [pii] AID - 10.3390/pharmaceutics18101330 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 20;18(10):1330. doi: 10.3390/pharmaceutics18101330. PMID- 42942859 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 20 TI - Cytoprotective Evaluation of Juglans regia L. Septum Extract and Development of Extract-Based Gels and Films for Topical Application. LID - 10.3390/pharmaceutics18101329 [doi] LID - 1329 AB - Background/Objectives:Juglans regia L. septa are a rich source of phenolic compounds with potential skin-related biological activity. This study evaluated the cytoprotective activity of a lyophilised J. regia septum extract and developed extract-containing polymeric gels suitable for topical application and semi-solid extrusion (SSE) 3D printing. Methods: The extract was characterised by high-performance liquid chromatography with photodiode array detection (HPLC-PDA) and evaluated in H(2)O(2)-exposed human fibroblasts. Poly(vinyl alcohol) (PVA)-, sodium carboxymethyl cellulose (CMC)-, and hydroxyethyl cellulose (HEC)-based gels were prepared, and the selected formulation was processed into films by solvent casting and SSE 3D printing. The films were evaluated for thickness, moisture content, mechanical properties, and probe-film adhesiveness. Results: Ellagic acid was the predominant quantified phenolic compound (36.453 microg/mL). The free extract protected fibroblasts against H(2)O(2)-induced oxidative damage, with the greatest preservation of viable cells at 75 and 100 microg/mL. Increasing extract loading increased total quantified phenolic content in the gels from 10.95 to 27.98 microg/mL. The selected formulation was successfully processed into multilayer films. 3D-printed films showed numerically lower burst strength and breaking force per unit width than solvent-cast films, although pairwise differences were not significant. Hydration increased probe-film adhesiveness, while film moisture content decreased during 3 months of storage. Conclusions: These findings support the feasibility of incorporating cytoprotective J. regia extract into printable polymeric gels for topical film development. FAU - Luksiene, Jurgita AU - Luksiene J AD - Department of Analytical and Toxicological Chemistry, Lithuanian University of Health Sciences, Sukileliu pr. 13, LT-50161 Kaunas, Lithuania. FAU - Rimkiene, Laura AU - Rimkiene L AUID- ORCID: 0009-0001-8694-594X AD - Department of Analytical and Toxicological Chemistry, Lithuanian University of Health Sciences, Sukileliu pr. 13, LT-50161 Kaunas, Lithuania. FAU - Majiene, Daiva AU - Majiene D AD - Department of Drug Technology and Social Pharmacy, Lithuanian University of Health Sciences, Sukileliu pr. 13, LT-50161 Kaunas, Lithuania. AD - Laboratory of Biochemistry, Neuroscience Institute, Lithuanian University of Health Sciences, LT-50162 Kaunas, Lithuania. FAU - Kazlauskaite, Jurga Andreja AU - Kazlauskaite JA AUID- ORCID: 0000-0002-7631-7122 AD - Department of Drug Technology and Social Pharmacy, Lithuanian University of Health Sciences, Sukileliu pr. 13, LT-50161 Kaunas, Lithuania. AD - Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, LT-50161 Kaunas, Lithuania. FAU - Bernatoniene, Jurga AU - Bernatoniene J AUID- ORCID: 0000-0003-1744-2932 AD - Department of Drug Technology and Social Pharmacy, Lithuanian University of Health Sciences, Sukileliu pr. 13, LT-50161 Kaunas, Lithuania. AD - Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, LT-50161 Kaunas, Lithuania. FAU - Zevzikovaite, Nerija AU - Zevzikovaite N AD - Department of Analytical and Toxicological Chemistry, Lithuanian University of Health Sciences, Sukileliu pr. 13, LT-50161 Kaunas, Lithuania. FAU - Zevzikovas, Andrejus AU - Zevzikovas A AUID- ORCID: 0009-0008-0660-5939 AD - Department of Analytical and Toxicological Chemistry, Lithuanian University of Health Sciences, Sukileliu pr. 13, LT-50161 Kaunas, Lithuania. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708517 OTO - NOTNLM OT - Juglans regia OT - by-product OT - extract OT - fibroblasts OT - films OT - gel OT - three-dimensional printing COIS- The authors confirm that they have no conflicts of interest to disclose. All co-authors have reviewed and agreed to the content of the manuscript, and there are no financial interests to declare. We assure that this submission is original work and is not currently being reviewed by any other publication. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:43 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - pharmaceutics18101329 [pii] AID - pharmaceutics-18-01329 [pii] AID - 10.3390/pharmaceutics18101329 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 20;18(10):1329. doi: 10.3390/pharmaceutics18101329. PMID- 42942853 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 19 TI - Toward Long-Acting Injectable Antidepressants: Drug-Delivery Strategies, Clinical Considerations, and Regulatory Barriers. LID - 10.3390/pharmaceutics18101323 [doi] LID - 1323 AB - Long-acting injectable (LAI) systems may offer an alternative approach to antidepressant treatment by reducing reliance on daily oral medication, supporting adherence, limiting pharmacokinetic fluctuations, and maintaining therapeutic drug exposure over extended periods. These potential advantages may be particularly relevant for patients with recurrent depression or difficulty sustaining regular oral treatment. However, converting conventional antidepressants into clinically useful LAIs remains challenging because many drugs require substantial maintenance doses, have formulation-limiting physicochemical properties, and may require dose adjustment or prompt discontinuation in response to adverse effects. This review evaluates the feasibility of established and emerging depot technologies, including polymeric microspheres, in situ-forming depots, lipid- and oil-based systems, hydrogels, and hybrid or stimulus-responsive platforms, for sustained antidepressant delivery. It compares these approaches according to drug-loading capacity, release mechanisms, achievable dosing interval, injection-volume constraints, manufacturing scalability, sterilization, product robustness, safety, local tissue tolerability, and regulatory maturity. Major translational barriers include the high total drug mass required for many antidepressants, acceptable injection-volume limits, solvent and excipient safety, injection-site reactions, limited reversibility after administration, pharmacokinetic drug-interaction risk, difficulty developing predictive in vitro-in vivo correlations and demanding regulatory expectations for complex injectable products. The review also considers opportunities for coordinated long-acting combination therapy, personalized patient selection, digital adherence monitoring, and emerging biomaterials. Overall, successful LAI antidepressant development will require careful matching of the drug molecules, delivery platform, pharmacokinetic target, and intended patient population. Early products are most likely to rely on established depot technologies and prior confirmation of oral efficacy and tolerability before long-term administration. FAU - Koppisetti, Haripriya AU - Koppisetti H AUID- ORCID: 0000-0001-8677-707X AD - Centre for Pharmaceutical Innovation, College of Health, Adelaide University, Adelaide, SA 5000, Australia. FAU - Rajapaksha, Weranga AU - Rajapaksha W AUID- ORCID: 0000-0001-8361-884X AD - Centre for Pharmaceutical Innovation, College of Health, Adelaide University, Adelaide, SA 5000, Australia. FAU - Nijhawan, Harsh P AU - Nijhawan HP AUID- ORCID: 0000-0002-8909-1871 AD - Centre for Pharmaceutical Innovation, College of Health, Adelaide University, Adelaide, SA 5000, Australia. FAU - Song, Yunmei AU - Song Y AUID- ORCID: 0000-0003-0008-5895 AD - Centre for Pharmaceutical Innovation, College of Health, Adelaide University, Adelaide, SA 5000, Australia. FAU - Youssef, Souha AU - Youssef S AUID- ORCID: 0000-0003-1670-6211 AD - Centre for Pharmaceutical Innovation, College of Health, Adelaide University, Adelaide, SA 5000, Australia. FAU - Garg, Sanjay AU - Garg S AUID- ORCID: 0000-0001-7253-2629 AD - Centre for Pharmaceutical Innovation, College of Health, Adelaide University, Adelaide, SA 5000, Australia. LA - eng PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708367 OTO - NOTNLM OT - antidepressants OT - lipid-based drug delivery systems OT - long-acting injectables (LAIs) OT - patient adherence OT - polymer-based drug delivery systems OT - sustained-release formulations COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:43 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - pharmaceutics18101323 [pii] AID - pharmaceutics-18-01323 [pii] AID - 10.3390/pharmaceutics18101323 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 19;18(10):1323. doi: 10.3390/pharmaceutics18101323. PMID- 42942850 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 18 TI - Drug Dosing During Continuous Renal Replacement Therapy in Critically Ill Patients: A Practical Narrative Review. LID - 10.3390/pharmaceutics18101320 [doi] LID - 1320 AB - Drug dosing during continuous renal replacement therapy (CRRT) is difficult in critically ill patients because drug exposure is influenced by both the patient and the extracorporeal treatment. This narrative review provides a practical overview of drug dosing during CRRT, while intermittent hemodialysis and sustained low-efficiency dialysis are discussed only where they provide relevant contextual or comparative information. It summarizes the main drug-related, patient-related, and treatment-related factors that influence extracorporeal drug clearance and discusses their clinical implications for commonly used drug classes. Loading and maintenance doses should be considered separately: loading doses depend mainly on volume of distribution and the need for early therapeutic exposure, whereas maintenance dosing depends on total clearance, including residual renal, extracorporeal, and non-renal clearance. Therapeutic drug monitoring can help guide dose adjustment when validated targets are available, while model-informed precision dosing may provide additional support in selected patients. The review also proposes an eight-step practical framework based on residual kidney function, the actual delivered CRRT prescription, drug pharmacokinetics and pharmacodynamics, therapeutic monitoring, and repeated reassessment. Future approaches may combine real-time CRRT data with pharmacokinetic models to support more individualized dosing. FAU - Pui, Mihai Claudiu AU - Pui MC AD - Doctoral School of Medicine and Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540139 Targu Mures, Romania. AD - Department of Anesthesiology and Intensive Care Medicine, Emergency County Hospital, 540136 Targu Mures, Romania. FAU - Lazar, Alexandra Elena AU - Lazar AE AD - Department of Anesthesiology and Intensive Care Medicine, Emergency County Hospital, 540136 Targu Mures, Romania. AD - Department of Anesthesiology and Intensive Care, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142 Targu Mures, Romania. FAU - Szederjesi, Janos AU - Szederjesi J AUID- ORCID: 0000-0001-9359-6058 AD - Department of Anesthesiology and Intensive Care Medicine, Emergency County Hospital, 540136 Targu Mures, Romania. AD - Department of Anesthesiology and Intensive Care, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142 Targu Mures, Romania. FAU - Branea, Oana Elena AU - Branea OE AUID- ORCID: 0009-0009-7616-3424 AD - Department of Anesthesiology and Intensive Care Medicine, Emergency County Hospital, 540136 Targu Mures, Romania. AD - Department of Anesthesiology and Intensive Care, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142 Targu Mures, Romania. FAU - Butiulca, Mihaela AU - Butiulca M AUID- ORCID: 0009-0003-9666-0862 AD - Department of Anesthesiology and Intensive Care Medicine, Emergency County Hospital, 540136 Targu Mures, Romania. AD - Department of Anesthesiology and Intensive Care, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142 Targu Mures, Romania. FAU - Cehan, Vlad-Dimitrie AU - Cehan VD AD - Department of Anesthesiology and Intensive Care Medicine, Emergency County Hospital, 540136 Targu Mures, Romania. AD - Department of Anesthesiology and Intensive Care, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142 Targu Mures, Romania. FAU - Straista, Mihaela AU - Straista M AD - Doctoral School of Medicine and Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540139 Targu Mures, Romania. AD - Department of Anesthesiology and Intensive Care Medicine, Emergency County Hospital, 540136 Targu Mures, Romania. FAU - Azamfirei, Leonard AU - Azamfirei L AUID- ORCID: 0000-0003-3220-2267 AD - Department of Anesthesiology and Intensive Care Medicine, Emergency County Hospital, 540136 Targu Mures, Romania. AD - Department of Anesthesiology and Intensive Care, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142 Targu Mures, Romania. LA - eng PT - Journal Article PT - Review DEP - 20261018 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708363 OTO - NOTNLM OT - continuous renal replacement therapy OT - drug dosing OT - model-informed precision dosing OT - pharmacokinetic variability OT - pharmacokinetics OT - renal replacement therapy OT - therapeutic drug monitoring COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:43 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - pharmaceutics18101320 [pii] AID - pharmaceutics-18-01320 [pii] AID - 10.3390/pharmaceutics18101320 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 18;18(10):1320. doi: 10.3390/pharmaceutics18101320. PMID- 42942847 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 17 TI - Gelled Oil-in-Water Microemulsions Stabilized with Natural Surfactant Decyl Glucoside as Cutaneous Nanocarriers for Cinnamon Bark Essential Oil: Formulation and Comprehensive Pharmacotechnical Characterization. LID - 10.3390/pharmaceutics18101317 [doi] LID - 1317 AB - Background/Objectives: Cinnamon bark essential oil (CEO) possesses antimicrobial, antioxidant, and anti-inflammatory properties. However, its topical application is limited by poor aqueous solubility, volatility, and chemical instability. The aim of this study was to develop gelled oil-in-water microemulsions stabilized with Decyl Glucoside for the cutaneous delivery of CEO and to evaluate their physicochemical and pharmaceutical characteristics. Methods: Pseudoternary phase diagrams were constructed to identify the microemulsion regions using Decyl Glucoside, propylene glycol, CEO, isopropyl myristate, and water. Selected CEO-loaded microemulsions were characterized in terms of droplet size, polydispersity index, zeta potential, refractive index, viscosity, and apparent pH, and were subsequently gelled with Aerosil((R)) 200. The resulting gelled microemulsions were evaluated for CEO content, apparent pH, rheological and textural properties, spreadability, in vitro release and skin permeation studies. The short-term stability of both liquid and gelled microemulsions was also assessed over 45 days. Results: Three CEO-loaded oil-in-water microemulsions were selected from the isotropic regions of the pseudoternary phase diagrams and successfully transformed into homogeneous gelled systems without phase separation. CEO incorporation increased the storage modulus and yield stress of the gelled formulations compared with the corresponding blank systems. The surfactant-to-cosurfactant ratio markedly influenced the rheological properties, mechanical strength, and in vitro release and skin permeation of CEO. Among the investigated formulations, MEG-CEO-2 provided the best balance between mechanical properties, CEO release, and skin permeation. Both liquid and gelled microemulsions maintained their principal physicochemical characteristics during the 45-day stability assessment. Conclusions: Decyl Glucoside-stabilized gelled microemulsions exhibited physicochemical and pharmaceutical properties supporting their further investigation as topical delivery systems for CEO. The results demonstrate that the surfactant-to-cosurfactant ratio can be used to tailor formulation performance, supporting a rational approach for the development of CEO-loaded dermal delivery systems. However, preservation of the biological activities of CEO following incorporation into these systems remains to be experimentally established. FAU - Zivkovic, Milja AU - Zivkovic M AD - Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia. FAU - Coneac, Georgeta-Hermina AU - Coneac GH AUID- ORCID: 0009-0008-0555-2978 AD - Department II-Pharmaceutical Technology, Formulation and Technology of Drugs Research Center, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square No. 2, 300041 Timisoara, Romania. FAU - Barjaktarevic, Ana AU - Barjaktarevic A AD - Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia. AD - Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia. FAU - Cupara, Snezana AU - Cupara S AD - Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia. FAU - Cotan, Ana-Maria AU - Cotan AM AD - Department II-Pharmaceutical Technology, Formulation and Technology of Drugs Research Center, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square No. 2, 300041 Timisoara, Romania. FAU - Vlaia, Lavinia-Lia AU - Vlaia LL AD - Department II-Pharmaceutical Technology, Formulation and Technology of Drugs Research Center, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square No. 2, 300041 Timisoara, Romania. FAU - Maxim, Monica Elisabeta AU - Maxim ME AD - Laboratory of Colloid Chemistry, "Ilie Murgulescu" Institute of Physical Chemistry, Romanian Academy, Splaiul Independentei 202, 060021 Bucharest, Romania. FAU - Precupas, Aurica AU - Precupas A AUID- ORCID: 0000-0002-2381-7617 AD - "Ilie Murgulescu" Institute of Physical Chemistry, Romanian Academy, Splaiul Independentei 202, 060021 Bucharest, Romania. FAU - Obradovic, Ksenija AU - Obradovic K AUID- ORCID: 0000-0002-2123-1540 AD - Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia. AD - Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia. FAU - Kostic, Marina AU - Kostic M AD - Department of Pharmacology and Toxicology, Faculty of Medical Sciences, University of Kragujevac, 34000 Kragujevac, Serbia. FAU - Vlaia, Vicentiu AU - Vlaia V AD - Department II-Organic Chemistry, Formulation and Technology of Drugs Research Center, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square No. 2, 300041 Timisoara, Romania. FAU - Neamu, Stefania AU - Neamu S AD - Pharmacy Doctoral School, Formulation and Technology of Drugs Research Center, Faculty of Pharmacy, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. FAU - Olariu, Ioana-Viorica AU - Olariu IV AD - Department II-Pharmaceutical Technology, Formulation and Technology of Drugs Research Center, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square No. 2, 300041 Timisoara, Romania. LA - eng PT - Journal Article DEP - 20261017 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708580 OTO - NOTNLM OT - cinnamon essential oil OT - cutaneous delivery OT - decyl glucoside OT - gelled microemulsion OT - oil-in-water microemulsion OT - skin permeation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:43 PHST- 2026/08/01 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - pharmaceutics18101317 [pii] AID - pharmaceutics-18-01317 [pii] AID - 10.3390/pharmaceutics18101317 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 17;18(10):1317. doi: 10.3390/pharmaceutics18101317. PMID- 42942844 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 16 TI - Cell Membrane-Coated Nanoparticles as Biomimetic Drug Delivery Systems: Design, Therapeutic Applications, and Translational Challenges. LID - 10.3390/pharmaceutics18101314 [doi] LID - 1314 AB - Biomimetic cell membrane-coated nanoparticles (CM-NPs) have emerged as a promising class of nanocarriers that integrate the tunable physicochemical properties of engineered nanoparticles with selected biological features of naturally derived cell membranes. By retaining membrane-associated proteins, lipids, glycans, and receptors, CM-NPs have demonstrated the potential to modulate immune recognition, prolong systemic circulation, improve biocompatibility, and facilitate disease-specific targeting; however, these effects depend on the membrane source, nanoparticle composition, coating integrity, fabrication method, and biological context. This review critically examines major membrane-engineering strategies, including lipid insertion, covalent conjugation, metabolic glycoengineering, and genetic modification, together with fabrication methods such as extrusion, sonication, electroporation, in situ polymerization, and microfluidics. Particular emphasis is placed on nanoparticles coated with erythrocyte, platelet, stem cell, immune cell, bacterial, cancer cell, and hybrid membranes. Their therapeutic applications are critically evaluated, with a primary focus on cancer therapy, including photothermal and photodynamic approaches, alongside applications in cardiovascular, neurological, infectious, inflammatory, and regenerative diseases. Recent CM-NP platforms are systematically summarized according to membrane source, therapeutic cargo or nanoparticle core, disease application, and major outcomes, while recent patents are discussed to highlight emerging technological and translational developments. Despite encouraging preclinical findings, clinical translation remains constrained by challenges in membrane isolation and purification, batch reproducibility, manufacturing scalability, preservation of membrane integrity and functionality, quality control, long-term stability, biosafety, and regulatory standardization. Advances in membrane engineering, hybrid membrane systems, scalable manufacturing, standardized characterization, and rigorous safety evaluation will be important for advancing CM-NPs toward clinical translation. FAU - Jacob, Shery AU - Jacob S AUID- ORCID: 0000-0003-1851-6673 AD - Department of Pharmaceutical Sciences, College of Pharmacy, Gulf Medical University, Ajman P.O. Box 4184, United Arab Emirates. FAU - Varkey, Namitha Raichel AU - Varkey NR AUID- ORCID: 0009-0000-7463-7088 AD - Department of Pharmaceutical Sciences, College of Pharmacy, Gulf Medical University, Ajman P.O. Box 4184, United Arab Emirates. FAU - Rao, Rekha AU - Rao R AUID- ORCID: 0000-0002-0525-4064 AD - Department of Pharmaceutical Sciences, Guru Jambheshwar University of Science and Technology, Hisar 125001, India. FAU - Shinu, Pottathil AU - Shinu P AUID- ORCID: 0000-0003-4245-0803 AD - Department of Biomedical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa 31982, Saudi Arabia. FAU - Khanam, Razia AU - Khanam R AUID- ORCID: 0000-0002-6434-5345 AD - Department of Biomedical Sciences, College of Medicine, Gulf Medical University, Ajman P.O. Box 4184, United Arab Emirates. LA - eng PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708389 OTO - NOTNLM OT - cancer therapy OT - cell membrane-coated nanoparticles OT - membrane surface engineering OT - photodynamic therapy OT - photothermal therapy OT - targeted drug delivery COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:43 PHST- 2026/09/26 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - pharmaceutics18101314 [pii] AID - pharmaceutics-18-01314 [pii] AID - 10.3390/pharmaceutics18101314 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 16;18(10):1314. doi: 10.3390/pharmaceutics18101314. PMID- 42942842 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 15 TI - Molecular Interactions of Bioactive Peptides with Proteins and DNA. LID - 10.3390/pharmaceutics18101311 [doi] LID - 1311 AB - Background: This study investigates the molecular recognition of two biologically active peptides (P1 and P2) by key biomacromolecular targets. The studied peptides were previously identified in Babylon snail hydrolysates and have been shown to exhibit antioxidant and cytotoxic activities against Caco-2 colorectal cancer cells. Given their reported biological potential, we decided to investigate both compounds from a different perspective. Their interactions with human serum albumin (HSA), alpha-1-acid glycoprotein (AAG), lysozyme (LYS), immunoglobulin G (IgG), and DNA were evaluated. Methods, Results and Conclusions: Spectroscopic analyses revealed concentration-dependent binding of the studied peptides to plasma proteins under investigations. Notably, peptide binding did not substantially alter the secondary structure of the target proteins, indicating that complex formation occurs without significant structural perturbation. Molecular docking further supported the formation of peptide-protein complexes, highlighting the contribution of hydrogen bonding and hydrophobic contacts to molecular recognition. The combined spectroscopic and docking results were consistent with a predominantly non-intercalative, possibly minor-groove-associated interaction with ctDNA. Taken together, these findings provide a comparative physicochemical characterization of the interactions of P1 and P2 with selected proteins and ctDNA. These findings provide insight into the molecular recognition properties of P1 and P2 and support further investigation of their biological relevance. FAU - Kotynia, Aleksandra AU - Kotynia A AUID- ORCID: 0000-0003-0730-8975 AD - Department of Basic Chemical Sciences, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211a, 50-556 Wroclaw, Poland. FAU - Krzyzak, Edward AU - Krzyzak E AUID- ORCID: 0000-0002-5000-2448 AD - Department of Basic Chemical Sciences, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211a, 50-556 Wroclaw, Poland. FAU - Czyznikowska, Zaneta AU - Czyznikowska Z AUID- ORCID: 0000-0002-5758-9667 AD - Department of Basic Chemical Sciences, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211a, 50-556 Wroclaw, Poland. FAU - Marciniak, Aleksandra AU - Marciniak A AUID- ORCID: 0000-0003-2225-7897 AD - Department of Basic Chemical Sciences, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211a, 50-556 Wroclaw, Poland. LA - eng GR - SUBZ.D290.26.010./Wroclaw Medical University/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708310 OTO - NOTNLM OT - acid glycoprotein OT - human immunoglobulin G OT - lysozyme OT - molecular docking OT - serum albumin OT - snail peptides OT - spectroscopic analyses COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:43 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - pharmaceutics18101311 [pii] AID - pharmaceutics-18-01311 [pii] AID - 10.3390/pharmaceutics18101311 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 15;18(10):1311. doi: 10.3390/pharmaceutics18101311. PMID- 42942840 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 15 TI - Gold Nanoparticle-Enabled Cancer Therapy: Translational Design Rules for Photothermal-Immunotherapy and Multimodal Precision Oncology. LID - 10.3390/pharmaceutics18101309 [doi] LID - 1309 AB - Gold nanoparticles (AuNPs) are versatile cancer-therapy platforms with tunable plasmonic properties, high atomic number, accessible surface chemistry, and potential for integration into theranostic systems. By controlling size, morphology, surface composition, and cargo-loading strategies, AuNPs can support photothermal therapy (PTT), chemotherapy delivery, nucleic-acid transport, radiosensitization, photodynamic therapy (PDT), sonodynamic therapy (SDT), and image-guided treatment. Among these applications, AuNP-mediated PTT is particularly attractive because localized hyperthermia can induce tumor-cell injury, support controlled cargo release, and, under appropriate conditions, contribute to immunogenic cell death and remodeling of the tumor immune microenvironment. However, promising preclinical findings have not translated into widespread routine oncology practice. Major barriers include heterogeneous tumor accumulation, limited penetration beyond perivascular regions, mononuclear phagocyte system uptake, uncertain long-term biodistribution and clearance, inadequate manufacturing reproducibility, and incomplete safety standardization. The enhanced permeability and retention effect is variable across tumors, lesions, and patients; it should not be regarded as a universal basis for clinical nanoparticle delivery. This review summarizes the physicochemical design principles governing AuNP biological performance and critically evaluates their major therapeutic uses in oncology. Particular emphasis is placed on photothermal-immunotherapy, tumor-microenvironment modulation, multimodal synergy, and pharmacokinetic, toxicological, manufacturing, and regulatory barriers. We propose a translational framework connecting material quality attributes, route of administration and in vivo fate, local energy delivery and thermal dosimetry, immune activation, patient selection, and clinically meaningful endpoints. This framework may guide development of more reproducible, controllable, and clinically relevant AuNP cancer therapies. FAU - Ma, Xianghua AU - Ma X AD - Jiangsu Hengrui Pharmaceuticals Co., Ltd., Lianyungang 222007, China. FAU - Xu, Wen AU - Xu W AD - Department of Microbiology, School of Basic Medical Sciences, Xi'an Medical University, Xi'an 710021, China. FAU - Chittasupho, Chuda AU - Chittasupho C AUID- ORCID: 0000-0002-0696-9969 AD - Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand. FAU - Zhang, Dian AU - Zhang D AUID- ORCID: 0009-0006-7676-966X AD - Department of Immunology, School of Basic Medical Sciences, Xi'an Medical University, Xi'an 710021, China. LA - eng GR - Chiang Mai University/ PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708672 OTO - NOTNLM OT - cancer nanomedicine OT - clinical translation OT - drug delivery OT - gold nanoparticles OT - photothermal therapy OT - radiosensitization OT - tumor immunotherapy OT - tumor microenvironment COIS- Author Xianghua Ma was employed by the company Jiangsu Hengrui Pharmaceuticals Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:43 PHST- 2026/08/22 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - pharmaceutics18101309 [pii] AID - pharmaceutics-18-01309 [pii] AID - 10.3390/pharmaceutics18101309 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 15;18(10):1309. doi: 10.3390/pharmaceutics18101309. PMID- 42942837 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 15 TI - The Stability-Release Paradox in Long-Acting Peptide and Protein Delivery: A Review of Material-Cargo Compatibility and Therapeutic Integrity. LID - 10.3390/pharmaceutics18101306 [doi] LID - 1306 AB - Long-acting peptide and protein delivery requires more than prolonged release; the therapeutic must remain chemically, physically, structurally, and functionally competent throughout processing, depot residence, and release. This review examines the stability-release paradox, in which material features introduced to extend residence can also alter cargo integrity. Rather than cataloging platforms or restating the need for biologic stabilization, the literature is organized around a cargo-specific compatibility question: whether a retention mechanism, depot microenvironment, and intended residence time remain compatible with preservation of therapeutic attributes required for activity. Published experimental studies and recent reviews available through 10 August 2026 were critically examined, with emphasis on poly(lactic-co-glycolic acid) (PLGA) systems, hydrogels, affinity-based depots, self-assembling formulations, and translational studies linking release with stability or pharmacological performance. Across these platforms, integrity is influenced by hydration, microclimate pH, reactive degradation products, drug-material affinity, interfacial exposure, local concentration, molecular crowding, processing stress, and host-depot interactions. Experimental studies of degradable polyesters show that prolonged mass release can coexist with peptide acylation or other chemical changes, whereas hydrogel and affinity systems demonstrate that reversible retention can suppress premature escape but still requires cargo-specific verification of recovery and biological function. The review therefore frames long-acting formulation design around three linked considerations: the depot as a dynamic pharmaceutical microenvironment, therapeutically competent release as an endpoint beyond cumulative mass recovery, and residence-time-stability matching as a compatibility test between planned confinement and the cargo's demonstrated stability window. This framework supports material selection and evaluation based on therapeutic integrity, stability-indicating release measurements, clinically relevant loading, and pharmacokinetic/pharmacodynamic (PK/PD) evidence appropriate to the therapeutic claim. FAU - Mumtaz, Sohail AU - Mumtaz S AUID- ORCID: 0000-0001-9506-5808 AD - Department of Chemical and Biological Engineering, Gachon University, 1342 Seongnamdaero, Sujeong-gu, Seongnam-si 13120, Republic of Korea. FAU - Afzal, Amir Muhammad AU - Afzal AM AD - Department of Physics, University of Okara, Okara 56300, Pakistan. LA - eng PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708117 OTO - NOTNLM OT - PLGA microspheres OT - hydrogel depots OT - long-acting injectables OT - peptide therapeutics OT - protein therapeutics OT - sustained release OT - therapeutic integrity COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:43 PHST- 2026/08/24 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - pharmaceutics18101306 [pii] AID - pharmaceutics-18-01306 [pii] AID - 10.3390/pharmaceutics18101306 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 15;18(10):1306. doi: 10.3390/pharmaceutics18101306. PMID- 42942836 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 15 TI - Functional Delivery of Nanoimmunotherapies in Pancreatic Ductal Adenocarcinoma: A Barrier-Informed Pharmaceutics Framework. LID - 10.3390/pharmaceutics18101305 [doi] LID - 1305 AB - Pancreatic ductal adenocarcinoma (PDAC) presents a stringent drug-delivery environment in which tumor accumulation does not necessarily translate into target-cell exposure or cargo activity. This critical narrative review develops a barrier-informed framework for evaluating nanoimmunotherapies across systemic exposure, tumor access, target-cell delivery, cargo release, target engagement, and pharmacological activity. The framework links vascular, stromal, immune, metabolic, and intracellular barriers to cargo-carrier selection, administration route, biological identity, pharmacokinetics, biodistribution, and intracellular trafficking. Platforms discussed include nanovaccines and nucleic-acid therapeutics, engineered extracellular vesicles, nanoengineered oncolytic-virus delivery, nanoparticle-assisted immune-cell programming, checkpoint modulation, and stromal or metabolic strategies relevant to immune-directed therapy. Functional-delivery evidence is integrated with critical quality attributes, mechanism-based potency, manufacturing comparability, biosafety, repeat-dose performance, and biomarker-guided clinical development. We propose a qualitative stage-gating framework based on concordant evidence of target-site exposure, target-cell activity, reproducible product quality, acceptable repeat-dose performance, and a testable biomarker strategy. This framework supports formulation selection, cross-platform comparison, and translational prioritization of PDAC nanoimmunotherapies. FAU - Xu, Baodan AU - Xu B AD - School of Medicine, Northwest University, Xi'an 710069, China. AD - Department of Hepatobiliary Surgery, Air Force Medical University, Xi'an 710032, China. FAU - Wei, Dan AU - Wei D AD - Department of Hepatobiliary Surgery, Air Force Medical University, Xi'an 710032, China. FAU - Tao, Kaishan AU - Tao K AD - Department of Hepatobiliary Surgery, Air Force Medical University, Xi'an 710032, China. FAU - Wang, Yang AU - Wang Y AD - Department of Hepatobiliary Surgery, Air Force Medical University, Xi'an 710032, China. LA - eng GR - 2023TQ0142/China Postdoctoral Science Foundation/ GR - No. 20250345/Shaanxi Association for Science and Technology/ GR - 2023JSYX09/Major Scientific and Technological Breakthroughs in Military and Aerospace Medicine/ PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708326 OTO - NOTNLM OT - biomarkers OT - critical quality attributes OT - drug delivery OT - formulation design OT - functional delivery OT - nanoimmunotherapy OT - nanomedicine OT - pancreatic ductal adenocarcinoma OT - pharmacokinetics OT - translational pharmaceutics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:43 PHST- 2026/08/12 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - pharmaceutics18101305 [pii] AID - pharmaceutics-18-01305 [pii] AID - 10.3390/pharmaceutics18101305 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 15;18(10):1305. doi: 10.3390/pharmaceutics18101305. PMID- 42942834 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 14 TI - Development and Physicochemical Characterization of Trans-Resveratrol Liposomes: Influence of Phospholipid Composition and Ultrasound-Assisted Processing. LID - 10.3390/pharmaceutics18101303 [doi] LID - 1303 AB - Background/Objectives: Trans-resveratrol is a bioactive polyphenol with limited pharmaceutical applicability due to poor aqueous solubility, chemical instability, and low bioavailability. This study aimed to develop trans-resveratrol-containing liposomal formulations and evaluate the influence of phospholipid composition and ultrasound-assisted processing (UAP) using a conventional ultrasonic bath on their physicochemical properties and storage behavior. Methods: Liposomes were prepared by the ethanol injection method using Lipoid((R)) S75, Lipoid((R)) P45, soybean lecithin, egg yolk lecithin, and selected binary phospholipid mixtures. Trans-resveratrol identity was verified by LC-MS. Particle size, polydispersity index (PDI), and zeta potential were determined before and after UAP, and physical stability was assessed after 30 days of refrigerated, light-protected storage. Results: Phospholipid composition markedly influenced liposome physicochemical characteristics. Lipoid((R)) P45-based formulations exhibited favorable initial particle size, PDI, and zeta potential profiles. UAP generally reduced particle size and improved particle size distribution immediately after preparation, although the magnitude of the effect depended on formulation composition. During refrigerated storage, short-term physical storage behavior was strongly formulation-dependent. Selected trans-resveratrol-containing formulations, particularly Lr12, Lr13, and Lr15, exhibited marked particle growth and increased polydispersity, whereas Lr20-Lr22 exhibited the most favorable particle size and PDI profiles after 30 days. Conclusions: Phospholipid composition was a critical determinant of trans-resveratrol liposome performance. Conventional ultrasonic-bath processing provided a simple and accessible means of improving initial liposomal characteristics, but did not universally ensure short-term physical stability during 30 days of refrigerated storage. Selected binary phospholipid formulations, particularly Lr20-Lr22, showed favorable physicochemical profiles and may represent promising candidates for further formulation development. FAU - Pudziuvelyte, Lauryna AU - Pudziuvelyte L AD - Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, LT-50161 Kaunas, Lithuania. AD - Department of Drug Technology and Social Pharmacy, Lithuanian University of Health Sciences, LT-50161 Kaunas, Lithuania. FAU - Paliulian, Agne AU - Paliulian A AD - Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, LT-50161 Kaunas, Lithuania. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708408 OTO - NOTNLM OT - ethanol injection OT - liposomes OT - particle size OT - phospholipids OT - polydispersity index OT - stability OT - trans-resveratrol OT - ultrasonic bath OT - ultrasound-assisted processing OT - zeta potential COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:43 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - pharmaceutics18101303 [pii] AID - pharmaceutics-18-01303 [pii] AID - 10.3390/pharmaceutics18101303 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 14;18(10):1303. doi: 10.3390/pharmaceutics18101303. PMID- 42942833 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 14 TI - From Target Discovery to Spatial Delivery: A Translational Roadmap for Gene-Targeted Therapies in Glioma. LID - 10.3390/pharmaceutics18101299 [doi] LID - 1299 AB - Diffuse gliomas are increasingly defined by molecular alterations, yet the clinical translation of gene-targeted therapies remains constrained by a persistent mismatch between target biology and spatial pharmacology. In this review, we connect target discovery to clinical development by integrating spatial and longitudinal tumor biology with payload selection, delivery engineering, biomarker design, manufacturing, and mechanism-driven trials. Recent clinical practices in locoregional CAR-T cells, oncolytic and gene-delivery viruses, tumor-homing myeloid cell gene therapy, mutation- and neoantigen-directed vaccination, RNA nanomedicines, and emerging genome-editing strategies suggest that vehicle and route of administration dramatically change drug exposure, therapeutic index, immune activity, and resistance. We therefore propose the concept of "delivery-adjusted targetability," in which a candidate target is judged not only by biological dependency but also by clonality, longitudinal stability, normal-brain expression, anatomical distribution, modality compatibility, and achievable target engagement. We further argue that the clinically actionable unit in diffuse glioma is often a target-anatomical compartment pair rather than a molecular target considered in isolation. Moreover, we advocate target-payload-vehicle-route-biomarker co-design and a stage-gated evidence package. This framework provides a potential way to compare otherwise dissimilar platforms, distinguish target failure from delivery failure, and reject biologically attractive but spatially implausible programs earlier. Treating molecular dependency and spatial access as a single design problem can make precision neuro-oncology more experimentally falsifiable and clinically actionable. FAU - Cheng, Hao AU - Cheng H AUID- ORCID: 0000-0002-7292-5198 AD - MOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Dalian 116024, China. FAU - Zhang, Yingchao AU - Zhang Y AD - MOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Dalian 116024, China. FAU - Zhu, Jinbo AU - Zhu J AUID- ORCID: 0000-0003-4935-825X AD - School of Biomedical Engineering, Faculty of Medicine, Dalian University of Technology, Dalian 116024, China. FAU - Yang, Yue AU - Yang Y AD - School of Biomedical Engineering, Faculty of Medicine, Dalian University of Technology, Dalian 116024, China. AD - Cancer Hospital of Dalian University of Technology (Liaoning Cancer Hospital and Institute, Cancer Hospital of China Medical University), Shenyang 110043, China. FAU - Lin, Jiaqi AU - Lin J AD - MOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Dalian 116024, China. LA - eng GR - 82404565/National Natural Science Foundation of China/ GR - 82601408/National Natural Science Foundation of China/ GR - BK20231018/Natural Science Foundation of Jiangsu Province/ GR - 2026-BS-0916/Natural Science Foundation of Liaoning Province/ PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708274 OTO - NOTNLM OT - blood-brain barrier OT - clinical translation OT - drug delivery OT - gene-targeted therapy OT - glioma OT - pharmacodynamics OT - spatial pharmacology COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:43 PHST- 2026/09/19 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - pharmaceutics18101299 [pii] AID - pharmaceutics-18-01299 [pii] AID - 10.3390/pharmaceutics18101299 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 14;18(10):1299. doi: 10.3390/pharmaceutics18101299. PMID- 42942832 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 14 TI - Response Surface Methodology-Guided Development of Hot-Melt-Extruded Cannabidiol Solid Self-Emulsifying Drug Delivery Systems for Enhanced Drug Release and Stability. LID - 10.3390/pharmaceutics18101302 [doi] LID - 1302 AB - Background/Objectives: Cannabidiol (CBD) is a non-psychoactive phytocannabinoid, with numerous therapeutic applications. It can be used to treat pain, inflammation, anxiety, and schizophrenia and it is also used to treat severe forms of infant epilepsy. CBD is a highly lipophilic compound with low oral bioavailability due to poor aqueous solubility and extensive first-pass metabolism. Self-emulsifying drug delivery systems (SEDDSs) offer a promising approach to enhancing the solubility and stability of lipophilic compounds like CBD. This study aimed to design and optimize CBD-loaded solid SEDDSs (S-SEDDSs) using Response Surface Methodology (RSM) to identify the optimal formulation parameters for improved drug release and stability. Methods: Excipients were selected through qualitative screening studies, considering their functional properties in SEDDS development. A Central Composite Design (CCD) was employed for response surface optimization to evaluate the effect of independent variables on formulation performance, including oil concentration and the surfactant-co-surfactant ratio. Formulations were processed using hot-melt extrusion (HME). The lead formulation was tested for drug content, in vitro drug release and stability studies. Results: The solid SEDDS formulation showed a drug content of 98.15 +/- 1.94% and achieved 76.6 +/- 0.6% drug release within three hours during in vitro dissolution testing, compared to 28 +/- 0.9% for pure CBD. During stability testing, the lead formulation showed minimal change at both 25 degrees C and 40 degrees C. Conclusions: Overall, the study demonstrated that RSM-guided formulation development combined with HME processing can produce CBD S-SEDDSs and improve in vitro drug release compared to pure CBD. FAU - Sanil, Kavish AU - Sanil K AD - Department of Pharmaceutics and Drug Delivery, School of Pharmacy, University of Mississippi, University, MS 38677, USA. FAU - Deshmukh, Janhavi AU - Deshmukh J AUID- ORCID: 0009-0003-6299-7192 AD - Department of Pharmaceutics and Drug Delivery, School of Pharmacy, University of Mississippi, University, MS 38677, USA. FAU - Ashour, Eman A AU - Ashour EA AUID- ORCID: 0000-0001-9013-1327 AD - Department of Pharmaceutics and Drug Delivery, School of Pharmacy, University of Mississippi, University, MS 38677, USA. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708488 OTO - NOTNLM OT - cannabidiol (CBD) OT - drug release OT - hot-melt extrusion OT - response surface methodology OT - self-emulsifying drug delivery system (SEDDS) OT - stability COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:43 PHST- 2026/09/11 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - pharmaceutics18101302 [pii] AID - pharmaceutics-18-01302 [pii] AID - 10.3390/pharmaceutics18101302 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 14;18(10):1302. doi: 10.3390/pharmaceutics18101302. PMID- 42942825 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 12 TI - Biochemical and Functional Characterization of LIZ, a Marine-Derived Serine Protease That Degrades Cross-Linked Fibrin and Attenuates Experimental Thrombosis. LID - 10.3390/pharmaceutics18101294 [doi] LID - 1294 AB - Background/Objectives: Direct fibrin-degrading proteases may provide a mechanistically distinct approach to clot proteolysis, but their development requires definition of catalytic behavior, fibrin selectivity, molecular identity, and safety. We characterized an activity-containing preparation from the marine polychaete Lepidasthenia izukai, designated LIZ. Methods: LIZ was obtained by ammonium sulfate fractionation and sequential chromatography. N-terminal sequencing, inhibitor profiling, chromogenic-substrate screening and S-2288 kinetics, fibrinogenolysis, direct fibrin-degradation assays, a kappa-carrageenan rat-tail thrombosis model, and cell viability were evaluated. Results: The final preparation showed a predominant band at approximately 28 kDa. N-terminal sequencing yielded IMGGSEAS, providing sequence-level characterization without definitive molecular identification. PMSF abolished activity and DFP reduced it to 9.8%, supporting a serine-protease biochemical profile. For S-2288, Km was 0.029 +/- 0.003 mM and apparent kcat/Km was 39.7 +/- 4.3 mM(-1) s(-1). LIZ degraded fibrinogen Aalpha within 30 s and Bbeta and gamma within 20 min, decreased pre-formed fibrin-polymer turbidity, and degraded FXIIIa-cross-linked fibrin. In the pretreatment model, thrombotic tail length was 0.7 +/- 1.0% with LIZ, 86.1 +/- 3.2% with kappa-carrageenan alone, and 0.5 +/- 0.6% with uPA (n = 3/group). Conclusions: LIZ shows direct activity against structurally stabilized fibrin and proof-of-concept antithrombotic activity. However, the data do not establish definitive molecular identity, therapeutic thrombolysis, fibrin selectivity, relative potency, or a therapeutic safety window. Further molecular, pharmacological, and safety studies are required. FAU - Yun, Sang-Gu AU - Yun SG AD - Department of Biomedical Science, College of Natural Sciences, Chosun University, Jeonnam-Gwangju Integrated Special City 61452, Republic of Korea. FAU - Park, Jung Eun AU - Park JE AUID- ORCID: 0000-0001-8789-9829 AD - Department of Biomedical Science, College of Natural Sciences, Chosun University, Jeonnam-Gwangju Integrated Special City 61452, Republic of Korea. AD - Department of Integrative Biological Science & BK21 FOUR Educational Research Group for Age-Associated Disorder Control Technology, Chosun University, Jeonnam-Gwangju Integrated Special City 61452, Republic of Korea. LA - eng GR - RS-2023-00248263 and RS-2026-25562669/National Research Foundation of Korea/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708678 OTO - NOTNLM OT - biopharmaceutical development OT - cross-linked fibrin OT - experimental thrombosis OT - fibrinogenolysis OT - fibrinolysis OT - marine enzyme OT - serine protease COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:43 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - pharmaceutics18101294 [pii] AID - pharmaceutics-18-01294 [pii] AID - 10.3390/pharmaceutics18101294 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 12;18(10):1294. doi: 10.3390/pharmaceutics18101294. PMID- 42942815 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 10 TI - Brazilian Red Propolis Extract-Loaded Colloidal Systems Derived from Microemulsion Compositions: Physicochemical, Antioxidant, and Antileishmanial Properties. LID - 10.3390/pharmaceutics18101284 [doi] LID - 1284 AB - Background/Objectives: Brazilian red propolis is a rich source of isoflavonoids with promising antioxidant and antileishmanial activities. However, its poor aqueous solubility and chemical instability limit its pharmaceutical application. This study aimed to develop microemulsion-based systems containing high concentrations of Brazilian red propolis extract (RPE) and evaluate their physicochemical properties, phytochemical composition, antioxidant capacity, and antileishmanial activity. Methods: Pseudoternary phase diagrams were constructed by water titration to select suitable microemulsion compositions. RPE was subsequently incorporated into the selected compositions to obtain RPE-loaded colloidal systems. The three selected RPE-free microemulsions and their corresponding RPE-loaded systems were characterized by pH, electrical conductivity, dynamic light scattering, rheological measurements, and polarized light microscopy (PLM). The RPE-loaded systems were further evaluated for phenolic marker content, antioxidant activity, and in vitro activity against Leishmania infantum. Results: Three RPE-loaded colloidal systems (F2-RPE12, F3-RPE30, and F4-RPE50) were developed. F2-RPE12 exhibited characteristics consistent with an oil-in-water microemulsion. Conductivity and PLM indicated physicochemical changes after RPE incorporation in F3-RPE30 and F4-RPE50, with localized birefringent features at higher RPE concentrations. Among the RPE-loaded systems, F4-RPE50 showed the highest phenolic and flavonoid contents and antioxidant activity. The RPE-loaded systems exhibited lower IC(50) values than RPE alone, with IC(50) values of 3.14, 2.28, and 0.56 microg.mL(-1) for F2-RPE12, F3-RPE30, and F4-RPE50, respectively, compared with 5.90 microg.mL(-1) for RPE. Conclusions: Overall, F4-RPE50 showed the most favorable phytochemical and biological performance, and the physicochemical and optical changes observed in the RPE-loaded systems open new avenues for investigating the relationship between structural organization and formulation performance. FAU - Costa, Salvana P M AU - Costa SPM AUID- ORCID: 0000-0003-0055-1219 AD - Post Graduate Program in Pharmaceutical Sciences, Institute of Pharmaceutical Science, Federal University of Alagoas, Maceio 57072-970, Alagoas, Brazil. AD - Post Graduate Program in Pharmaceutical Sciences, Department of Life Sciences, State University of Bahia, Av. Silveira Martins, 2555, Cabula, Salvador 41150-000, BA, Brazil. FAU - Barreto, Ighor C AU - Barreto IC AUID- ORCID: 0000-0001-6144-8181 AD - Environmental Coordination Office, Federal University of Bahia, Av. Adhemar de Barros, Ondina, Salvador 40170-110, BA, Brazil. FAU - Santos, Kathylen V F Dos AU - Santos KVFD AUID- ORCID: 0009-0005-8803-4807 AD - Post Graduate Program in Pharmaceutical Sciences, Department of Pharmaceutical Sciences, Federal University of Sao Paulo, Rua Sao Nicolau, 20, Centro, Diadema 09913-030, SP, Brazil. FAU - Gama, Nataly C S AU - Gama NCS AUID- ORCID: 0009-0004-2396-0618 AD - Post Graduate Program in Pharmaceutical Sciences, Institute of Pharmaceutical Science, Federal University of Alagoas, Maceio 57072-970, Alagoas, Brazil. FAU - Vila Nova, Monique A AU - Vila Nova MA AUID- ORCID: 0000-0003-2819-2334 AD - Post Graduate Program in Pharmaceutical Sciences, Institute of Pharmaceutical Science, Federal University of Alagoas, Maceio 57072-970, Alagoas, Brazil. FAU - Sarmento, Victor Hugo V AU - Sarmento VHV AUID- ORCID: 0000-0001-6882-002X AD - Department of Chemistry, Federal University of Sergipe, Avenida Vereador Olimpio Grande, S/N, Professor Alberto Carvalho Campus, Itabaiana 49500-000, SE, Brazil. FAU - Rocha, Louisianny G da AU - Rocha LGD AUID- ORCID: 0009-0000-5694-843X AD - Parasitology Laboratory, Center for Biosciences, Federal University of Rio Grande do Norte, Lagoa Nova, Natal 59010-180, RN, Brazil. FAU - Basilio, Irinaldo D Jr AU - Basilio ID Jr AUID- ORCID: 0000-0003-2385-3842 AD - Post Graduate Program in Pharmaceutical Sciences, Institute of Pharmaceutical Science, Federal University of Alagoas, Maceio 57072-970, Alagoas, Brazil. FAU - Panjwanig, Camila M B R G AU - Panjwanig CMBRG AUID- ORCID: 0000-0003-0203-3079 AD - School of Dentistry, Federal University of Alagoas, Av. Lourival Melo Mota, S/N, Tabuleiro do Martins, Maceio 57072-900, AL, Brazil. FAU - Nascimento, Ticiano G do AU - Nascimento TGD AUID- ORCID: 0000-0002-3856-8764 AD - Post Graduate Program in Pharmaceutical Sciences, Institute of Pharmaceutical Science, Federal University of Alagoas, Maceio 57072-970, Alagoas, Brazil. LA - eng GR - Edital DT 2025 (process number: 304765/2025-2)/CNPq/ GR - Edital 02/2022, grant number: E:60030.0000002389/2022/FAPEAL/ GR - Edital 11/2022, Grant number: E:60030.0000000327/2023)/FAPEAL/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708299 OTO - NOTNLM OT - Leishmania infantum OT - antioxidant activity OT - colloids OT - microemulsions OT - propolis OT - surfactant COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:43 PHST- 2026/07/07 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - pharmaceutics18101284 [pii] AID - pharmaceutics-18-01284 [pii] AID - 10.3390/pharmaceutics18101284 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 10;18(10):1284. doi: 10.3390/pharmaceutics18101284. PMID- 42942813 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 9 TI - Progress in Anti-Obesity Drug Development: A Pharmacodynamics Perspective. LID - 10.3390/pharmaceutics18101281 [doi] LID - 1281 AB - Background/Objectives: Obesity is a chronic, multifactorial condition driven by complex neuroendocrine and metabolic pathways that limit the long-term success of lifestyle interventions. Pharmacotherapy has advanced from early monoaminergic agents to incretin-based and multi-receptor agonists with substantially improved efficacy. This review aims to provide pharmacodynamics-focused synthesis of current and emerging anti-obesity medications, emphasizing mechanistic targets, receptor interactions, and translational implications. Methods: We conducted a narrative review integrating Phase I-III clinical trials, mechanistic pharmacology data (receptor affinity, potency, and signaling bias), and regulatory summaries for FDA-approved and selected emerging, investigational, repurposed, or regionally approved anti-obesity agents. Inclusion focused on clinical relevance, pharmacodynamic novelty, and weight-loss efficacy. Results: Approved anti-obesity medications act through diverse pathways, including the inhibition of fat absorption (orlistat), the modulation of central appetite circuits (phentermine/topiramate, naltrexone/bupropion), and incretin-based mechanisms (liraglutide, semaglutide, tirzepatide, and the oral GLP-1 receptor agonists semaglutide 25 mg and the nonpeptide orforglipron). GLP-1 receptor agonists achieve approximately 6-15% weight loss, while dual glucose-dependent insulinotropic polypeptide/GLP-1 receptor agonists such as tirzepatide reach 15-20%. Emerging agents-including triple agonists (retatrutide), glucagon-based co-agonists, amylin analogs with Phase III efficacy data, earlier-stage growth differentiation factor 15 (GDF15) pathway agonists, and peripherally restricted cannabinoid-1 receptor inverse agonists-demonstrate enhanced metabolic effects and potential improvements in lean-mass preservation. Obesity-related alterations in pharmacokinetics and pharmacodynamics highlight the need for individualized dosing strategies. Conclusions: Clinical efficacy and tolerability cannot be inferred from receptor identity alone. They emerge from relative receptor potency and efficacy, exposure, tissue access, receptor trafficking, and mechanism-linked adverse effects. Dual and triple agonists can broaden efficacy, but receptor balance and standardized comparative pharmacology remain essential for rational development and individualized dosing. FAU - Ahmad, Ishtiaque AU - Ahmad I AD - Research and Innovation Institute, Ministry of Defense Health Services, Riyadh 12426, Saudi Arabia. FAU - Azmi, Sarfuddin AU - Azmi S AUID- ORCID: 0000-0002-1868-3458 AD - Research and Innovation Institute, Ministry of Defense Health Services, Riyadh 12426, Saudi Arabia. FAU - Albabtain, Monirah A AU - Albabtain MA AUID- ORCID: 0000-0001-5163-5987 AD - Department of Pharmacy, Prince Sultan Cardiac Center, Riyadh 12426, Saudi Arabia. FAU - Mustafa, Mohammad AU - Mustafa M AD - Research and Innovation Institute, Ministry of Defense Health Services, Riyadh 12426, Saudi Arabia. FAU - Kunnathodi, Faisal AU - Kunnathodi F AUID- ORCID: 0000-0002-5958-3672 AD - Research and Innovation Institute, Ministry of Defense Health Services, Riyadh 12426, Saudi Arabia. FAU - Anvarbatcha, Riyasdeen AU - Anvarbatcha R AD - Research and Innovation Institute, Ministry of Defense Health Services, Riyadh 12426, Saudi Arabia. FAU - AlWhaiby, Bander R AU - AlWhaiby BR AD - Research and Innovation Institute, Ministry of Defense Health Services, Riyadh 12426, Saudi Arabia. FAU - Alharbi, Emtenan M AU - Alharbi EM AUID- ORCID: 0009-0009-0842-9534 AD - Clinical Pharmacy Department, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia. FAU - Arafat, Amr A AU - Arafat AA AUID- ORCID: 0000-0003-0951-7287 AD - Research and Innovation Institute, Ministry of Defense Health Services, Riyadh 12426, Saudi Arabia. AD - Department of Adult Cardiac Surgery, Prince Sultan Cardiac Center, Riyadh 12426, Saudi Arabia. FAU - Alotaibi, Haifa F AU - Alotaibi HF AUID- ORCID: 0000-0003-2266-7394 AD - Research and Innovation Institute, Ministry of Defense Health Services, Riyadh 12426, Saudi Arabia. AD - Department of Family Medicine, Prince Sultan Military Medical City, Riyadh 12426, Saudi Arabia. LA - eng PT - Journal Article PT - Review DEP - 20261009 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708705 OTO - NOTNLM OT - GLP-1 agonists OT - anti-obesity medication OT - energy homeostasis OT - incretin therapy OT - multi-receptor agonists OT - obesity pharmacotherapy OT - pharmacodynamics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:43 PHST- 2026/08/12 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - pharmaceutics18101281 [pii] AID - pharmaceutics-18-01281 [pii] AID - 10.3390/pharmaceutics18101281 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 9;18(10):1281. doi: 10.3390/pharmaceutics18101281. PMID- 42942810 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 9 TI - Retatrutide as a Unimolecular GIP/GLP-1/Glucagon Receptor Tri-Agonist: Molecular Design, Pharmaceutical Development and Critical Interpretation of Emerging Phase 3 Evidence. LID - 10.3390/pharmaceutics18101277 [doi] LID - 1277 AB - Retatrutide (LY3437943) is an investigational, once-weekly, lipidated 39-residue peptide that activates the glucose-dependent insulinotropic polypeptide, glucagon-like peptide-1, and glucagon receptors. Its molecular design combines incretin activity with glucagon receptor engagement while maintaining glycaemic efficacy. This critical narrative review examines molecular engineering, pharmacokinetics, dose escalation, pharmaceutical development, and clinical evidence available through 11 September 2026. Five phase 3 trials have reported positive primary outcomes, but only TRANSCEND-T2D-1 has a full peer-reviewed report; the obesity trials are currently available as sponsor topline disclosures. In those disclosures, efficacy-estimand mean weight reductions of 28.3% at 80 weeks (TRIUMPH-1) and 28.7% at 68 weeks (TRIUMPH-4) were reported, with a TRIUMPH-1 treatment-regimen estimate of 25.0%; these values remain provisional pending full published trial reports. The peer-reviewed TRANSCEND-T2D-1 report supports glycaemic efficacy in early type 2 diabetes. Interpretation requires attention to estimands, population differences, treatment discontinuation and selection into trial extensions. Gastrointestinal adverse events, dysaesthesia, heart-rate changes and the functional consequences of lean-mass loss require further evaluation. Pharmaceutical priorities include scalable synthesis, impurity and potency control, formulation stability, immunogenicity and reliable dose delivery. Retatrutide provides a clinically advanced example of unimolecular poly-agonism, but comparative effectiveness, long-term outcomes and regulatory review remain essential to defining its therapeutic role. FAU - Mateescu, Diana-Maria AU - Mateescu DM AUID- ORCID: 0009-0009-4951-1702 AD - Department of Infectious Diseases, Municipal Emergency Clinical Hospital Timisoara, 2A Hector Street, 300041 Timisoara, Romania. FAU - Capraru, Ionut Dragos AU - Capraru ID AD - Department of Epidemiology, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041 Timisoara, Romania. FAU - Enache, Bogdan AU - Enache B AUID- ORCID: 0000-0003-2806-9002 AD - Department of Cardiology, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Square 2, 300041 Timisoara, Romania. FAU - Enache, Alexandra AU - Enache A AD - Institute of Legal Medicine Timisoara, 1A Ciresului Street, 300610 Timisoara, Romania. AD - Ethics and Human Identification Research Center, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Square 2, 300041 Timisoara, Romania. AD - Discipline of Forensic Medicine, Bioethics, Deontology, and Medical Law, Department of Neuroscience, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Square 2, 300041 Timisoara, Romania. FAU - Precup, Cris Virgiliu AU - Precup CV AUID- ORCID: 0009-0009-4299-5652 AD - Department of Biology and Life Sciences, Faculty of Medicine, "Vasile Goldis" Western University of Arad, 310025 Arad, Romania. FAU - Buhas, Camelia Liana AU - Buhas CL AUID- ORCID: 0000-0003-0184-4259 AD - Department of Morphological Disciplines, Faculty of Medicine and Pharmacy, University of Oradea, 410087 Oradea, Romania. FAU - Muresan, Camelia-Oana AU - Muresan CO AUID- ORCID: 0009-0006-3896-9599 AD - Institute of Legal Medicine Timisoara, 1A Ciresului Street, 300610 Timisoara, Romania. AD - Ethics and Human Identification Research Center, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Square 2, 300041 Timisoara, Romania. AD - Discipline of Forensic Medicine, Bioethics, Deontology, and Medical Law, Department of Neuroscience, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Square 2, 300041 Timisoara, Romania. LA - eng GR - Victor Babes University of Medicine and Pharmacy Timisoara/ PT - Journal Article PT - Review DEP - 20261009 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708278 OTO - NOTNLM OT - GIP OT - GLP-1 OT - LY3437943 OT - glucagon receptor OT - lipidation OT - obesity OT - peptide therapeutics OT - pharmaceutical development OT - retatrutide OT - triple agonist OT - type 2 diabetes COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:43 PHST- 2026/09/11 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - pharmaceutics18101277 [pii] AID - pharmaceutics-18-01277 [pii] AID - 10.3390/pharmaceutics18101277 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 9;18(10):1277. doi: 10.3390/pharmaceutics18101277. PMID- 42942809 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 9 TI - 3D-Printed Gastroretentive Floating Modified-Release Device for Dual Cardiovascular Drug Delivery: Isosorbide Dinitrate and Hydralazine. LID - 10.3390/pharmaceutics18101278 [doi] LID - 1278 AB - Background: Isosorbide dinitrate (ISDN) and hydralazine hydrochloride (HDZ) are dual cardiovascular drugs used to treat heart failure. However, the drugs have limitations such as short plasma half-life, low bioavailability, and potential for tolerance of the nitrate component. To address the need for prolonged drug release, a floating dual-compartment drug delivery system was developed to provide controlled drug release under simulated gastric dissolution conditions. Objectives: This study aimed to develop floating dual-compartment devices (FDCDs) to contain ISDN and HDZ gel and modify drug release under simulated gastric conditions. Methods: The FDCD was designed in a cylindrical shape consisting of an air chamber on the upper part for floating ability, and two compartments for loaded drug gel and controlling the drug release rate. Fused deposition modeling (FDM) was selected to fabricate FDCDs using polylactic acid (PLA) filament. The ISDN formulation varied the ratio of polyvinylpyrrolidone (PVP) to achieve drug release for 12 h. The HDZ formulation was optimized by varying the ratio of PVP and hydroxypropyl methylcellulose (HPMC) to extend drug release at 24 h. Results: The shape dimension and weight variation in the FDCDs showed low standard deviation (SD). FDCDs exhibited immediate floating, resisted overturning, and demonstrated in vitro floating ability for more than 24 h. The formulation ISDN3 at a PVP: drug: water ratio of 5:2:3 exhibited complete release at 12 h, while the formulation of HDZ1 at an HPMC: PVP: drug: water ratio of 0.3:2.5:2:5.2 showed complete release within 24 h. The ISDN3- and HDZ1-loaded FDCDs demonstrated prolonged release behavior, with ISDN and HDZ release profiles showing the best fit to the zero-order and Higuchi models, respectively, based primarily on the coefficient of determination (R(2)), while root mean square error (RMSE) was used as a complementary measure of fitting error. Conclusions: These findings suggest that the developed dual-drug-loaded FDCD has potential as a dual-drug oral delivery platform for simultaneous delivery of ISDN and HDZ with prolonged drug release characteristics and further development of oral combination drug delivery systems. FAU - Jaiseri, Dareena AU - Jaiseri D AUID- ORCID: 0009-0005-3866-8879 AD - Pharmaceutical Development of Green Innovations Group (PDGIG) and Division of Industrial Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. FAU - Pengnam, Supusson AU - Pengnam S AUID- ORCID: 0000-0003-4664-0276 AD - Pharmaceutical Development of Green Innovations Group (PDGIG) and Division of Biopharmaceutical Sciences and Pharmacology, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. AD - Research and Innovation Center for Advanced Therapy Medicinal Products, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. FAU - Opanasopit, Praneet AU - Opanasopit P AUID- ORCID: 0000-0002-4878-2529 AD - Pharmaceutical Development of Green Innovations Group (PDGIG) and Division of Industrial Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. AD - Research and Innovation Center for Advanced Therapy Medicinal Products, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. FAU - Suriyaamporn, Phuvamin AU - Suriyaamporn P AUID- ORCID: 0000-0002-6944-9514 AD - Pharmaceutical Development of Green Innovations Group (PDGIG) and Division of Industrial Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. AD - Research and Innovation Center for Advanced Therapy Medicinal Products, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. FAU - Pornpitchanarong, Chaiyakarn AU - Pornpitchanarong C AUID- ORCID: 0000-0001-6540-280X AD - Pharmaceutical Development of Green Innovations Group (PDGIG) and Division of Industrial Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. AD - Research and Innovation Center for Advanced Therapy Medicinal Products, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. FAU - Rojanarata, Theerasak AU - Rojanarata T AD - Pharmaceutical Development of Green Innovations Group (PDGIG) and Division of Industrial Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. AD - Research and Innovation Center for Advanced Therapy Medicinal Products, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. FAU - Pamornpathomkul, Boonnada AU - Pamornpathomkul B AD - Pharmaceutical Development of Green Innovations Group (PDGIG) and Division of Industrial Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. AD - Research and Innovation Center for Advanced Therapy Medicinal Products, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. FAU - Patrojanasophon, Prasopchai AU - Patrojanasophon P AUID- ORCID: 0000-0002-4974-4532 AD - Pharmaceutical Development of Green Innovations Group (PDGIG) and Division of Industrial Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. AD - Research and Innovation Center for Advanced Therapy Medicinal Products, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. FAU - Kansom, Teeratas AU - Kansom T AUID- ORCID: 0000-0002-3014-6807 AD - Pharmaceutical Development of Green Innovations Group (PDGIG) and Division of Industrial Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. AD - Research and Innovation Center for Advanced Therapy Medicinal Products, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. FAU - Charoenying, Thapakorn AU - Charoenying T AUID- ORCID: 0000-0003-2419-1676 AD - Pharmaceutical Development of Green Innovations Group (PDGIG) and Division of Industrial Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. AD - Research and Innovation Center for Advanced Therapy Medicinal Products, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. LA - eng GR - N41A661139/National Research Council of Thailand/ GR - Thailand Science Research and Innovation (TSRI) National Science, Research and Innovation Fund (NSRF) (Fiscal Year 2025)/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708162 OTO - NOTNLM OT - 3D printing OT - cardiovascular drugs OT - gastroretentive floating drug delivery systems OT - hydralazine OT - isosorbide dinitrate COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:43 PHST- 2026/08/12 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - pharmaceutics18101278 [pii] AID - pharmaceutics-18-01278 [pii] AID - 10.3390/pharmaceutics18101278 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 9;18(10):1278. doi: 10.3390/pharmaceutics18101278. PMID- 42942807 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 8 TI - Development of Pectin-Coated Cationic Liposomes for Sustained Drug Release, Enhanced Serum Stability and Reduced Macrophage Uptake. LID - 10.3390/pharmaceutics18101275 [doi] LID - 1275 AB - Background: Cationic liposomes are promising carriers for anticancer agents but are often limited by rapid clearance by the mononuclear phagocyte system, poor serum stability, and insufficient control of drug release. This study aimed to develop and characterize pectin-coated, Ca(2+)-crosslinked cationic liposomes as a multifunctional delivery platform providing sustained release, enhanced serum stability, and reduced macrophage uptake while preserving the biological activity of an encapsulated anticancer compound. Methods: The optimal polysaccharide-to-liposome ratio was established by zeta-potential polyelectrolyte titration, and coating parameters were optimized using a Taguchi experimental design. Physicochemical characterization included dynamic light scattering (DLS), transmission electron microscopy (TEM), isothermal titration calorimetry (ITC), small-angle X-ray scattering (SAXS), and differential scanning calorimetry (DSC). The optimized formulations were further evaluated for drug release, serum stability, cellular uptake and cytotoxicity against A549 and HeLa cells using MTT and Neutral Red assays. Results: Polyelectrolyte titration identified a pectin-to-liposome saturation ratio of 3:1, and Taguchi optimization yielded a formulation with a drug retention efficiency of 99.34%. Pectin coating significantly reduced the initial burst release (37.9% at 1 h) and decreased cumulative drug release after 48 h (66.3%), consistent with a polymer relaxation-controlled release mechanism. The coated liposomes also exhibited enhanced serum stability and reduced macrophage uptake (up to ~73% lower in RAW264.7 cells), while maintaining uptake by non-phagocytic cells. The biological activity of the encapsulated model compound was preserved, with enhanced cancer-cell selectivity against A549 and HeLa cells demonstrated by both MTT and Neutral Red assays. Conclusions: Pectin-Ca(2+) surface functionalization transforms cationic liposomes into a stable sustained-release delivery platform with reduced macrophage recognition and enhanced serum stability. These findings support the further development of this carrier as a versatile platform for the systemic delivery of anticancer agents. FAU - Simeonov, Plamen AU - Simeonov P AUID- ORCID: 0009-0009-2333-9879 AD - Department of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, Medical University of Plovdiv, 15A Vasil Aprilov blvd, 4002 Plovdiv, Bulgaria. AD - Research Institute at Medical University of Plovdiv (RIMU), 4002 Plovdiv, Bulgaria. FAU - Todorova, Velislava AU - Todorova V AUID- ORCID: 0000-0001-9840-6890 AD - Research Institute at Medical University of Plovdiv (RIMU), 4002 Plovdiv, Bulgaria. AD - Department of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University of Plovdiv, 15A Vasil Aprilov blvd, 4002 Plovdiv, Bulgaria. FAU - Batsalova, Tsvetelina AU - Batsalova T AUID- ORCID: 0000-0002-6560-1945 AD - Department of Developmental Biology, Faculty of Biology, Paisii Hilendarski University of Plovdiv, 24 Tsar Assen Str., 4000 Plovdiv, Bulgaria. FAU - Dzhambazov, Balik AU - Dzhambazov B AUID- ORCID: 0000-0001-8052-5840 AD - Department of Developmental Biology, Faculty of Biology, Paisii Hilendarski University of Plovdiv, 24 Tsar Assen Str., 4000 Plovdiv, Bulgaria. FAU - Ivanova, Stanislava AU - Ivanova S AUID- ORCID: 0000-0003-1282-7868 AD - Research Institute at Medical University of Plovdiv (RIMU), 4002 Plovdiv, Bulgaria. AD - Department of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University of Plovdiv, 15A Vasil Aprilov blvd, 4002 Plovdiv, Bulgaria. FAU - Katsarov, Plamen AU - Katsarov P AUID- ORCID: 0000-0002-9158-6208 AD - Department of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, Medical University of Plovdiv, 15A Vasil Aprilov blvd, 4002 Plovdiv, Bulgaria. AD - Research Institute at Medical University of Plovdiv (RIMU), 4002 Plovdiv, Bulgaria. LA - eng PT - Journal Article DEP - 20261008 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708220 OTO - NOTNLM OT - coated liposomes OT - pectin coating OT - reduced macrophage uptake OT - sustained drug release COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:43 PHST- 2026/08/07 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - pharmaceutics18101275 [pii] AID - pharmaceutics-18-01275 [pii] AID - 10.3390/pharmaceutics18101275 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 8;18(10):1275. doi: 10.3390/pharmaceutics18101275. PMID- 42942806 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 8 TI - Beyond the Needle: Transforming Traditional Healthcare with Microneedle Drug Delivery Systems. LID - 10.3390/pharmaceutics18101274 [doi] LID - 1274 AB - In the present era, modern drug delivery systems play a fundamental role in ensuring the systematic delivery of the drug into the body, reaching the desired targets. Across recent decades, the drug delivery system has evolved from simple oral and injectable formulations to interpolating with various developed techniques to fabricate innovative treatment approaches for safer, effective, and better patient compliance. Progressive techniques like nanoparticles, microparticles, liposomes, polymer-based systems, 3D printing, and biological carriers have been extensively combined with specifically targeted drug-delivery techniques across infectious, oncological, pulmonary, and neurological conditions. Microneedle transdermal patches have advanced in tandem. They have gained attention for their advancement to surpass the stratum corneum, minimal invasiveness, independent delivery, and patient compliance. This review provides a translational perspective by integrating MN materials and device engineering with drug-delivery performance, patient usability, clinical evidence, manufacturing scalability, and regulatory considerations to examine the progression of MN technologies from laboratory development toward real-world healthcare implementation. Microneedles are fabricated in different types, such as solid, coated, hollow, dissolving, and hydrogel-based, to enhance precision in dosing, better permeability, and suitability across diverse populations, and complex molecules are designed, such as proteins, peptides, hormones, vaccines, and cosmetic agents. Moreover, studies suggest MN are widely accepted by geriatric and pediatric groups, reduce anxiety, and significantly benefit individuals with trypanophobia, swallowing difficulties in the case of oral doses, and compromised venous action, in addition to breaking social inequity. MN-induced microchannels can transiently increase skin permeability and may facilitate sustained or controlled drug delivery depending on MN geometry, formulation, application conditions, skin site, and individual skin characteristics. The persistence of these microchannels and their safety profile are dependent on both device and formulation characteristics. MN is a pivotal part of the novel drug delivery system, addresses the complications arising due to conventional drug delivery, and proves to focus on the improvement of pharmacokinetic parameters, patient-centric therapy, target delivery, and real-world accessibility. Further transformation in the enhancement of MN technology can lead to clinically promising drug delivery systems for the management of long-term treatment and boost next-generation therapies. FAU - Manivannan, Arjun Gokulan AU - Manivannan AG AD - Department of Pharmacology, SRM College of Pharmacy, Faculty of Medicine and Health Sciences, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu 603203, Tamil Nadu, India. FAU - Jayasankar, Narayanan AU - Jayasankar N AUID- ORCID: 0000-0002-6149-2761 AD - Department of Pharmacology, SRM College of Pharmacy, Faculty of Medicine and Health Sciences, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu 603203, Tamil Nadu, India. FAU - Kashid, Vivekanand Ankush AU - Kashid VA AUID- ORCID: 0009-0001-8467-2183 AD - MABD Institute of Pharmaceutical Education and Research, Babhulgaon, Yeola, Nashik 423401, Maharashtra, India. AD - Centre for Research Impact and Outcome, Chitkara College of Pharmacy, Chitkara University, Rajpura 140401, Punjab, India. FAU - Patel, Ravish J AU - Patel RJ AUID- ORCID: 0000-0002-8442-0349 AD - Ramanbhai Patel College of Pharmacy, CHARUSAT Campus Charotar University of Science and Technology, Anand 388421, Gujarat, India. FAU - G Prajapati, Bhupendra AU - G Prajapati B AUID- ORCID: 0000-0001-8242-4541 AD - Department of Pharmaceutics, Parul Institute of Pharmacy, Faculty of Pharmacy, Parul University, Waghodia, Vadodara 391760, Gujarat, India. AD - Department of Industrial Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. LA - eng GR - CRFPM002/SRM Institute of Science and Technology/ PT - Journal Article PT - Review DEP - 20261008 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708118 OTO - NOTNLM OT - improved bioavailability OT - microneedle OT - polymers OT - transdermal drug delivery systems COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:43 PHST- 2026/08/18 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - pharmaceutics18101274 [pii] AID - pharmaceutics-18-01274 [pii] AID - 10.3390/pharmaceutics18101274 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 8;18(10):1274. doi: 10.3390/pharmaceutics18101274. PMID- 42942803 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 7 TI - Oxaprozin-Based Amide Derivatives as Multifunctional Agents: Synthesis, Characterization, and Comprehensive Biological Profiling. LID - 10.3390/pharmaceutics18101271 [doi] LID - 1271 AB - Background/Objectives: Inflammation plays a role at multiple levels, contributing to tumorigenesis, cellular proliferation, cell survival, and metastasis. The established link between chronic inflammation and tumor development has shifted the therapeutic focus toward nonsteroidal anti-inflammatory drugs, which exert their effects primarily through cyclooxygenase inhibition. Methods: In the present study, the antitumor potential of novel synthesized oxaprozin derivatives was evaluated using the MTT assay. The immunomodulatory effects of the selected derivative were determined in vitro by measuring cytokine concentrations and performing flow cytometry. To investigate the inhibitory capacity of these derivatives against cyclooxygenase enzymes, an in vitro inhibition assay was conducted using fluorometric screening kits, followed by in silico validation. Results: All tested tumor cell lines exhibited sensitivity to compound 1, which demonstrated the lowest cytotoxic effect against non-cancerous mMSCs and MRC-5 cells. This compound can effectively target tumor cells, inducing potent apoptosis in 4T1 breast cancer cells through a strategic shift in the Bax/Bcl-2 balance and the downregulation of the oncogenic driver Bcl-6. Although the synthesized oxaprozin derivatives showed moderate affinity for COX-1, none of the newly synthesized compounds achieved more than 50% inhibition against COX-2 at a concentration of 100 muM. Consistently, in silico procedures validated the results of the in vitro COX inhibition assay. Conclusions: The findings of this study indicate that compound 1 has the ability to modulate immune responses under stimulated conditions, and its potent antitumor activity may not be directly related to cyclooxygenase inhibition, suggesting the involvement of COX-independent pathways. FAU - Gogic, Andela AU - Gogic A AD - Department of Medical Statistics and Informatics, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. FAU - Nikolic, Milos AU - Nikolic M AUID- ORCID: 0000-0002-6960-3438 AD - Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. AD - Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. FAU - Nedeljkovic, Nikola AU - Nedeljkovic N AUID- ORCID: 0000-0003-4560-2630 AD - Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. AD - Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. FAU - Vesovic, Marina AU - Vesovic M AUID- ORCID: 0000-0002-7177-4666 AD - Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. AD - Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. FAU - Zivanovic, Ana AU - Zivanovic A AD - Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. AD - Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. FAU - Dobricic, Vladimir AU - Dobricic V AUID- ORCID: 0000-0002-5086-9017 AD - Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia. FAU - Boskovic, Jelena AU - Boskovic J AD - Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia. FAU - Kostic, Isidora AU - Kostic I AUID- ORCID: 0009-0000-8308-8885 AD - Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. FAU - Marinkovic, Jovana Z AU - Marinkovic JZ AUID- ORCID: 0009-0007-6203-6657 AD - Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. FAU - Jurisevic, Milena AU - Jurisevic M AD - Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. AD - Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. FAU - Gajovic, Nevena AU - Gajovic N AUID- ORCID: 0000-0003-0535-2964 AD - Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. FAU - Simovic Markovic, Bojana AU - Simovic Markovic B AUID- ORCID: 0000-0001-8408-4624 AD - Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. FAU - Zdravkovic, Nebojsa AU - Zdravkovic N AUID- ORCID: 0000-0003-0877-3684 AD - Department of Medical Statistics and Informatics, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. FAU - Jovanovic, Ivan AU - Jovanovic I AUID- ORCID: 0000-0002-1169-2378 AD - Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. AD - Faculty of Medicine, University of East Sarajevo, Studentska 5, 73300 Foca, Bosnia and Herzegovina. AD - Institute of Public Health Kragujevac, Nikole Pasica 1, 34000 Kragujevac, Serbia. LA - eng GR - 451-03-33/2026-03/200161/Ministry of Science, Technological Development, and Innovation/ GR - 451-03-34/2026-03/200161/Ministry of Science, Technological Development, and Innovation/ GR - 451-03-33/2026-03/200111/Ministry of Science, Technological Development, and Innovation/ GR - 451-03-34/2026-03/200111/Ministry of Science, Technological Development, and Innovation/ GR - JP 14/20/University of Kragujevac/ GR - JP 07/23/University of Kragujevac/ GR - JP 06/24/University of Kragujevac/ PT - Journal Article DEP - 20261007 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708156 OTO - NOTNLM OT - COX-1 OT - COX-2 OT - amide derivatives OT - cytotoxicity OT - immunomodulatory effect OT - molecular docking OT - oxaprozin COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/07 CRDT- 2026/10/27 10:43 PHST- 2026/09/13 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - pharmaceutics18101271 [pii] AID - pharmaceutics-18-01271 [pii] AID - 10.3390/pharmaceutics18101271 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 7;18(10):1271. doi: 10.3390/pharmaceutics18101271. PMID- 42942800 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 7 TI - Permeability Is the Key in Sublingual and Buccal Drug Delivery. LID - 10.3390/pharmaceutics18101268 [doi] LID - 1268 AB - Sublingual and buccal drug delivery has evolved from a route reserved for a handful of cardiovascular and analgesic agents into an actively investigated non-invasive delivery platform within pharmaceutical sciences [...]. FAU - Alfawaz, Rand Musa Obaid AU - Alfawaz RMO AUID- ORCID: 0009-0000-3296-7425 AD - College of Pharmacy, King Saud bin Abdul Aziz University of Health Sciences (KSAU-HS), Riyadh 11481, Saudi Arabia. FAU - Almoazen, Hassan AU - Almoazen H AUID- ORCID: 0000-0001-8059-1637 AD - Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, 881 Madison Ave., Rm 661, Memphis, TN 38163, USA. LA - eng PT - Editorial DEP - 20261007 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708702 COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/07 CRDT- 2026/10/27 10:43 PHST- 2026/08/24 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - pharmaceutics18101268 [pii] AID - pharmaceutics-18-01268 [pii] AID - 10.3390/pharmaceutics18101268 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 7;18(10):1268. doi: 10.3390/pharmaceutics18101268. PMID- 42942796 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 6 TI - Impact of Process Parameters on the Quality Attributes of High-Dose Metformin Hydrochloride Film-Coated Tablets. LID - 10.3390/pharmaceutics18101264 [doi] LID - 1264 AB - Background/Objectives: Formulating high-dose metformin hydrochloride film-coated tablets is challenging due to the poor flowability and compressibility inherent to high drug loading. To ensure process robustness and consistent product quality, understanding the impact of manufacturing variables is crucial. This study evaluated the effects of key process parameters on the quality attributes of 1000 mg metformin hydrochloride tablets to establish optimal processing conditions for reliable production. Methods: The manufacturing process, comprising wet granulation, compression, and film coating, was investigated to evaluate the influence of key process parameters on tablet quality attributes. Granules were characterized for bulk density, tapped density, and compressibility index. Core tablets were evaluated for physical appearance, weight variation, hardness, and dissolution at a 45 min sampling point across various compression forces and tableting speeds. Film-coating efficiency was assessed based on coating quality, dissolution behavior, and stability. Favorable manufacturing conditions were empirically identified through single-variable optimization and statistical analysis. Results: Wet granulation conducted at a chopper speed of 1400 rpm for 7 min yielded granules with the lowest compressibility index (11.88%), demonstrating significantly improved flowability. Compression at a force of 10 kN and a speed of 40 rpm produced core tablets with acceptable appearance, robust hardness (7.06-7.37 kP), and complete drug release (100%). Tablet hardness was governed by a strong interaction between compression force and tableting speed (partial eta(2) = 0.929, p < 0.001) rather than by either factor alone: at 20 kN, hardness collapsed sharply once tableting speed reached 30 rpm, coincident with mild capping, identifying this force-speed combination as a process-failure condition. For the coating process, maintaining a tablet bed surface temperature of 40 degrees C ensured optimal coating uniformity without compromising product quality. Dissolution profile comparison against a coated reference product in the primary dissolution medium (pH 6.8) yielded f2 similarity values above 50 for all eight core tablet conditions, and stability testing under accelerated/thermal-stress conditions (up to 65 degrees C) confirmed maintained dissolution, assay, and related-substance (1-cyanoguanidine) compliance for both the test and reference products. The optimized manufacturing conditions successfully produced film-coated tablets that conformed to all predetermined quality specifications. Conclusions: Manufacturing process parameters significantly influenced the quality attributes of high-dose metformin hydrochloride film-coated tablets. Proper selection and control of these processing conditions ensured consistent granule properties, optimal tablet quality, and enhanced manufacturing robustness. FAU - Duc, Nguyen Tan AU - Duc NT AD - Faculty of Pharmacy, Can Tho University of Medicine and Pharmacy, Can Tho 900000, Vietnam. FAU - Nguyen, Dang-Khoa AU - Nguyen DK AUID- ORCID: 0000-0002-6681-7609 AD - School of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City 700000, Vietnam. FAU - Liet, Huynh Thanh AU - Liet HT AD - DHG Pharmaceutical Joint-Stock Company, Can Tho 900000, Vietnam. FAU - Thiet, Nguyen Huynh AU - Thiet NH AD - DHG Pharmaceutical Joint-Stock Company, Can Tho 900000, Vietnam. FAU - Thang, Phan Nguyen Truong AU - Thang PNT AUID- ORCID: 0009-0004-4256-8072 AD - Institute of Drug Quality Control-Ho Chi Minh City (IDQC HCMC), Ho Chi Minh City 700000, Vietnam. FAU - Lu, Phu Loc AU - Lu PL AUID- ORCID: 0009-0000-8767-0266 AD - Faculty of Pharmacy, Can Tho University of Medicine and Pharmacy, Can Tho 900000, Vietnam. FAU - Ly, Ngoc Hanh AU - Ly NH AUID- ORCID: 0009-0008-1947-3154 AD - Faculty of Pharmacy, Can Tho University of Medicine and Pharmacy, Can Tho 900000, Vietnam. FAU - Huynh, Duyen Thi My AU - Huynh DTM AUID- ORCID: 0000-0003-3306-3051 AD - Faculty of Pharmacy, Can Tho University of Medicine and Pharmacy, Can Tho 900000, Vietnam. LA - eng PT - Journal Article DEP - 20261006 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708723 OTO - NOTNLM OT - film coating OT - high-dose tablets OT - manufacturing process parameters OT - metformin hydrochloride OT - tablet quality attributes OT - wet granulation COIS- Authors Nguyen Tan Duc, Huynh Thanh Liet, Nguyen Huynh Thiet were employees by the company DHG Pharmaceutical Joint-Stock Company. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/06 CRDT- 2026/10/27 10:43 PHST- 2026/09/14 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/06 00:00 [pmc-release] AID - pharmaceutics18101264 [pii] AID - pharmaceutics-18-01264 [pii] AID - 10.3390/pharmaceutics18101264 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 6;18(10):1264. doi: 10.3390/pharmaceutics18101264. PMID- 42942791 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 5 TI - Dose Optimization of Cefazolin for Pediatric Patients with Renal Impairment Using Physiologically Based Pharmacokinetic Modeling (PBPK) in GastroPlus. LID - 10.3390/pharmaceutics18101260 [doi] LID - 1260 AB - Background/Objectives: Cefazolin is a first-generation cephalosporin antibiotic widely used for the treatment and prophylaxis of bacterial infections in both adult and pediatric populations. It is predominantly eliminated unchanged by the kidneys. Impaired renal function can reduce its clearance and increase systemic exposure. This study aimed to develop and validate a PBPK model of cefazolin using GastroPlus 9.9 to optimize dosing in pediatric patients with renal impairment. Methods: A cefazolin PBPK model incorporating renal transport processes was developed using clinical data from healthy adults, subsequently evaluated in adults with renal impairment, and scaled to healthy pediatrics following validation in these populations. The model was extrapolated to pediatric patients with varying degrees of renal impairment. Dose optimization was performed based on predicted exposure, followed by pharmacodynamic evaluation using unbound fractions and a selected MIC of 4 microg/mL. Results: The model demonstrated good agreement between observed and predicted pharmacokinetic parameters including C(max) and AUC(0-infinity) across the evaluated populations. Dose reductions of approximately 43%, 80%, and 90% relative to the standard pediatric dose were identified in moderate RI, severe RI, and ESRD, respectively, together with interval extension. Predicted %fT > MIC was 55.5%, 58%, 83%, and 81% for mild RI, moderate RI, severe RI, and ESRD, respectively. For surgical prophylaxis, unbound cefazolin concentrations remained above the MIC for 4.5-38.8 h across the RI groups, supporting maintenance above the MIC during a simulated 4 h surgical period. Conclusions: The PBPK model adequately describes cefazolin pharmacokinetic in healthy adults, adults with CKD, and healthy pediatric subjects and provides a model-informed basis for dose and interval adjustment in pediatric RI. The proposed regimens are model-based extrapolations requiring clinical confirmation, especially because the pediatric RI model was not independently validated against observed pediatric RI data. FAU - Abdeen, Abdelrahman AU - Abdeen A AD - College of Pharmacy, Al Ain University, Al Ain P.O. Box 64141, United Arab Emirates. FAU - Bin Jardan, Yousef A AU - Bin Jardan YA AUID- ORCID: 0000-0003-3302-4036 AD - Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia. FAU - Rahim, Najia AU - Rahim N AUID- ORCID: 0000-0002-9874-8134 AD - Department of Pharmacy Practice, Dow College of Pharmacy, Dow University of Health Sciences, Karachi 74200, Pakistan. FAU - Alshami, Ruqaia AU - Alshami R AD - College of Pharmacy, Al Ain University, Al Ain P.O. Box 64141, United Arab Emirates. FAU - Lobenberg, Raimar AU - Lobenberg R AUID- ORCID: 0000-0002-0919-0213 AD - 3142K Katz Group Centre for Research, Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB T6G 2H5, Canada. FAU - Sarfraz, Muhammad AU - Sarfraz M AUID- ORCID: 0000-0002-0516-4966 AD - College of Pharmacy, Al Ain University, Al Ain P.O. Box 64141, United Arab Emirates. LA - eng GR - ORF-2026-457/King Saud University/ PT - Journal Article DEP - 20261005 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708336 OTO - NOTNLM OT - PBPK OT - fold errors OT - model development OT - pediatrics OT - transporters COIS- The authors declare that they have no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/05 CRDT- 2026/10/27 10:43 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/05 00:00 [pmc-release] AID - pharmaceutics18101260 [pii] AID - pharmaceutics-18-01260 [pii] AID - 10.3390/pharmaceutics18101260 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 5;18(10):1260. doi: 10.3390/pharmaceutics18101260. PMID- 42942789 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 5 TI - Biogenic Selenium Nanoparticle-Based Nanocomposite Hydrogel for Dual Delivery of Curcumin and Simvastatin for Topical Wound Healing Applications. LID - 10.3390/pharmaceutics18101257 [doi] LID - 1257 AB - Background: Chronic and ischemic wounds are dysregulated, causing inflammation, oxidative stress, and bacterial colonization. This work developed a bionanocomposite hydrogel using biogenic selenium nanoparticles (SeNPs) to encapsulate and topically distribute two weakly water-soluble medicines, curcumin (Curc) and simvastatin (SMV), to improve wound healing. Methods: Green production of biogenic SeNPs utilizing Psidium guajava (guava) leaf extract. Based on entrapment efficiency (%EE; E1) and cumulative drug release after 2 and 8 h (E2) and (E3), a two-factor, three-level Box-Behnken design selected the SeNP-to-drug weight ratio (C(1)) and ethanol concentration (C(2)). The selected nanoconstruct was integrated into a 2% w/v Carbopol 934 hydrogel and characterized by UV-vis, FTIR, SEM, and DLS. In a 14-day full-thickness excisional wound model in Wistar rats, wound contraction, tissue remodeling, inflammatory responses, and healing kinetics were examined to determine its therapeutic efficiency. Results: Formulation F9 had the highest overall attractiveness (0.998) and %EE values of 94.71% for SMV and 93.92% for Curc. DLS showed a hydrodynamic particle size of 177.7 nm and PDI of 0.41. Both medications were successfully incorporated without chemical incompatibility based on FTIR analysis. The selected nanogel maintained a skin-friendly pH of 6.2 +/- 0.1 and sustained biphasic drug release. In vivo, the half-dose nanogel showed an enhanced wound-healing response, accelerating wound contraction and achieving complete epithelialization in 14 +/- 1.06 days (p < 0.05) compared to 22 +/- 2.5 days for the sham control, corresponding to a 36% reduction in healing time. Histopathology showed epidermal thickness restoration and well-organized collagen deposition. In addition, therapy dramatically decreased the proinflammatory biomarkers IL-1beta, MMP-9, and COX-2 and increased IL-10 levels without causing tissue discomfort. Conclusions: The biogenic SeNP-based nanogel delivers Curc and SMV locally with prolonged drug release and anti-inflammatory and tissue-remodeling effects. The half-dose combination accelerated wound healing while retaining tissue compatibility, suggesting this green nanocomposite hydrogel could be a promising topical method for advanced cutaneous wound therapy. FAU - Kasem, Nahla A AU - Kasem NA AUID- ORCID: 0009-0008-1589-9860 AD - Department of Pharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Sohag University, Sohag 82524, Egypt. AD - Department of Pharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Sohag National University, Sohag 82524, Egypt. FAU - Sarhan, Hatem A AU - Sarhan HA AD - Department of Pharmaceutics, Faculty of Pharmacy, Minia University, Minia 61519, Egypt. FAU - Mostafa, Mahmoud AU - Mostafa M AUID- ORCID: 0000-0001-6425-9247 AD - Department of Pharmaceutics, Faculty of Pharmacy, Minia University, Minia 61519, Egypt. FAU - Hofny, Heba A AU - Hofny HA AD - Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sohag University, Sohag 82524, Egypt. AD - Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sohag National University, Sohag 82524, Egypt. FAU - Abouzed, Deiaa E Elsayed AU - Abouzed DEE AD - Department of Pharmacology and Toxicology, Faculty of Pharmacology, Sohag University, Sohag 82524, Egypt. FAU - Elsayed, Mahmoud M A AU - Elsayed MMA AUID- ORCID: 0000-0001-9547-2243 AD - Department of Pharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Sohag University, Sohag 82524, Egypt. AD - Department of Pharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Sohag National University, Sohag 82524, Egypt. FAU - Mostafa, Mahmoud Elkot AU - Mostafa ME AUID- ORCID: 0009-0002-5378-100X AD - Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, New Valley University, Al-Kharga 72511, Egypt. AD - Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, New Valley National University, Al-Kharga 72511, Egypt. LA - eng PT - Journal Article DEP - 20261005 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708416 OTO - NOTNLM OT - Box-Behnken optimization OT - dual-drug delivery system OT - molecular docking simulation OT - selenium nanoparticles OT - wound healing COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/05 CRDT- 2026/10/27 10:43 PHST- 2026/09/04 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/05 00:00 [pmc-release] AID - pharmaceutics18101257 [pii] AID - pharmaceutics-18-01257 [pii] AID - 10.3390/pharmaceutics18101257 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 5;18(10):1257. doi: 10.3390/pharmaceutics18101257. PMID- 42942788 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 3 TI - Physiologically Constrained Deep Learning in Physiologically Based Pharmacokinetic Modeling: Virtual Patient Generation, Machine-Learned Scaling Laws, and Pediatric Precision Dosing. LID - 10.3390/pharmaceutics18101256 [doi] LID - 1256 AB - Physiologically based pharmacokinetic (PBPK) modeling is now central to pediatric dose selection, because clinical sampling in children is ethically constrained and simple weight-based scaling is unreliable while organ function and enzyme activity are still maturing. Three bottlenecks limit classical PBPK in this setting, namely building physiologically coherent virtual patient populations, extrapolating drug disposition across species and age, and estimating many mechanism-linked parameters from sparse data. Over the past five years, deep learning has begun to address each. Generative models constrained by physiological knowledge create virtual patients that preserve realistic correlations and whole-body coherence. Graph networks and neural ordinary differential equations learn continuous scaling and maturation relationships instead of relying on fixed exponents. Physics-informed networks and surrogate emulators estimate mechanistic parameters and quantify uncertainty in data-poor pediatric settings. This review surveys these developments, organizes them by the PBPK workflow they serve, and links them to precision dosing of three antiepileptic drugs, lacosamide, lamotrigine, and oxcarbazepine. Unlike earlier surveys focused on concentration prediction and therapeutic drug monitoring, we keep the mechanistic model in the foreground, treating the virtual patient as the unit of simulation. We also discuss open problems, including validation standards for generated populations, the regulatory qualification of learning-enhanced PBPK, uncertainty reporting, and the prospect of foundation models of pharmacokinetics. Throughout, we distinguish methodological maturity from demonstrated clinical benefit. FAU - Zhang, Lei AU - Zhang L AD - Department of Pharmacy, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310009, China. FAU - Xu, Yichao AU - Xu Y AUID- ORCID: 0000-0002-0476-9787 AD - Center of Clinical Pharmacology, The Second Affiliated Hospital, Zhejiang University School of Medicine, 88 Jiefang Road, Hangzhou 310009, China. LA - eng GR - 82504944/the Youth Science Fund Project of the National Natural Science Foundation of China/ GR - 2025KY849/the Health Commission of Zhejiang Province Project/ GR - 2023ZYY12/the Zhejiang Pharmaceutical Association Hospital Pharmacy Special Research Project/ PT - Journal Article PT - Review DEP - 20261003 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708350 OTO - NOTNLM OT - antiepileptic drugs OT - generative deep learning OT - pediatric precision dosing OT - physiologically based pharmacokinetic modeling OT - virtual patients COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/03 CRDT- 2026/10/27 10:43 PHST- 2026/08/28 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - pharmaceutics18101256 [pii] AID - pharmaceutics-18-01256 [pii] AID - 10.3390/pharmaceutics18101256 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 3;18(10):1256. doi: 10.3390/pharmaceutics18101256. PMID- 42942781 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Oct 1 TI - Understanding the Role of Solvents in Hypromellose-Based Film-Forming Solutions for Topical Administration of Ketoprofen Through a Design of Experiments Approach. LID - 10.3390/pharmaceutics18101249 [doi] LID - 1249 AB - Background/Objectives: Topical ketoprofen gels are limited by the need for frequent reapplication, short skin residence time, and the risk of sunlight-induced photoallergic dermatitis. Film-forming solutions (FFSs) allow for extended retention; however, the solvents used may affect the polymer film's characteristics. The present study aimed to elucidate the effect of macrogol 400 and isopropanol and develop a hypromellose-based ketoprofen FFS using a design of experiments (DoE) approach. Methods: A 3(2) full factorial design was applied to investigate the effects of macrogol 400 (0-5%, w/w) (X(1)) and isopropanol (25-50%, w/w) concentrations (X(2)) on film mechanical properties (tensile strength (Y(1)), elongation at break (Y(2)), and Young's modulus (Y(3))). The DoE-based placebo and ketoprofen-loaded FFSs were characterized for their physicochemical, rheological, and textural properties, while the corresponding films were evaluated for appearance, weight, stickiness, and mechanical properties. In vitro ketoprofen release from the drug-loaded FFS was also evaluated. Results: Macrogol 400 concentration was identified as a significant factor influencing film mechanical properties. As a film plasticizer, macrogol 400 improved film mechanical properties up to the optimal concentration (2.75%), whereas the water/isopropanol ratio mainly affected the applicative (rheological and textural) properties of the FFSs. Ketoprofen incorporation significantly reduced the film tensile strength and elongation at break. In vitro release testing demonstrated 58.52% ketoprofen release after 6 h, with the Weibull model showing the best fit to the release profile. Conclusions: Macrogol 400 and isopropanol have a complex, mutually conditioned influence on the application characteristics of hypromellose-based FFS and the mechanical characteristics of the films. Incorporation of ketoprofen into the selected placebo formulation significantly reduced film deformability but yielded a thin, transparent film with favorable in vitro drug-release characteristics. The developed FFS represents a promising topical dosage form for non-steroidal anti-inflammatory drugs and warrants further investigation, particularly in terms of skin retention and in vivo performance. FAU - Milinkovic, Sandra AU - Milinkovic S AUID- ORCID: 0009-0008-2545-702X AD - Department of Pharmaceutical Technology and Cosmetology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia. FAU - Kurcubic, Ivana AU - Kurcubic I AD - Department of Pharmaceutical Technology and Cosmetology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia. FAU - Duris, Jelena AU - Duris J AUID- ORCID: 0000-0002-1833-6704 AD - Department of Pharmaceutical Technology and Cosmetology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia. FAU - Dekic, Ljiljana AU - Dekic L AD - Department of Pharmaceutical Technology and Cosmetology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia. LA - eng GR - 451-03-33/2026-03/200161/Ministry of Science, Technological Development, and Innovation/ GR - 451-03-34/2026-03/200161/Ministry of Science, Technological Development, and Innovation/ PT - Journal Article DEP - 20261001 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708587 OTO - NOTNLM OT - design of experiments OT - film-forming solution OT - full factorial design OT - hypromellose OT - in vitro drug release OT - ketoprofen OT - mechanical properties COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:43 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - pharmaceutics18101249 [pii] AID - pharmaceutics-18-01249 [pii] AID - 10.3390/pharmaceutics18101249 [doi] PST - epublish SO - Pharmaceutics. 2026 Oct 1;18(10):1249. doi: 10.3390/pharmaceutics18101249. PMID- 42942776 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Sep 30 TI - Propolis-Based Microneedles Incorporating Neratinib-Loaded Ocimum basilicum Essential Oil-Based Lipid Nanovesicles for Minimally Invasive Cancer Therapy: Formulation and In Vivo Evaluation in an Ehrlich Solid Tumor Model. LID - 10.3390/pharmaceutics18101244 [doi] LID - 1244 AB - Background: Neratinib (NTB) is a potent pan-HER inhibitor, but its oral bioavailability is limited due to poor aqueous solubility, low permeability, and gastrointestinal toxicity. This study incorporated a bioactive transdermal system by incorporating NTB-loaded nanovesicles of Ocimum basilicum oil (NTB-OOs) into dissolving microneedles of PVA/PVP-propolis. Methods: Ocimum basilicum oil was characterized by gas chromatography-mass spectrometry (GC-MS). NTB-OOs were screened by I-optimal design and evaluated for vesicular properties, stability, release, skin permeation, and cytotoxicity. The optimized vesicles were loaded in propolis microneedles and evaluated mechanically, ex vivo, and in MCF-7 cells and Ehrlich solid carcinoma-bearing mice. Results: GC-MS revealed a volatile profile rich in linalool, methyl jasmonate, and a cyclopenta[g]-2-benzopyran derivative with reported antioxidant and anticancer activities. The optimized NTB-OOs showed a particle size of (198.9 +/- 0.98 nm), entrapment efficiency of (88.9 +/- 0.45%), PDI of (0.16 +/- 0.01), zeta potential of (-23.1 +/- 0.35 mV), and six-month stability. The microneedles provided zero-order release (48-50% after 24 h) and ex vivo permeation (~3500 microg/cm(2)), which were 2.7- and 1.2-fold higher than those of the NTB suspension and NTB-OOs, respectively. NTB-OOs reduced MCF-7 IC(50) from 11.1 to 5.1 microg/mL. In vivo, NTB-OOs/propolis microneedles achieved 75.6% tumor inhibition, increased mean survival from 19 to 26.5 days, and raised the T/C ratio to 139.5%. They also induced apoptosis and downregulated the expression of HER-2, EGFR, VEGF-A, and Bcl-2. Conclusions: The multifunctional nanovesicle-propolis microneedle platform improves NTB solubilization, controlled release, dermal delivery, and antitumor efficacy, supporting its potential for minimally invasive localized breast cancer therapy. FAU - Elhabal, Sammar Fathy AU - Elhabal SF AD - Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Modern University for Technology and Information (MTI), Mokattam, Cairo 11571, Egypt. FAU - Awad AbdelGhany Morsy, Suzan AU - Awad AbdelGhany Morsy S AUID- ORCID: 0000-0002-5874-194X AD - Department of Clinical Pharmacology, Faculty of Medicine, Alexandria University, Alexandria 21526, Egypt. AD - Pathological Sciences Department, MBBS Program, Fakeeh College for Medical Sciences, Jeddah 21461, Saudi Arabia. FAU - Azer, Marian Sobhy AU - Azer MS AUID- ORCID: 0000-0003-1072-9893 AD - Department of Pharmaceutics, College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology (MUST), 6th of October City 12566, Egypt. FAU - Allam, Khaled M AU - Allam KM AUID- ORCID: 0000-0002-3442-1124 AD - Department of Pharmacognosy, Faculty of Pharmacy, Qena University, Qena 83523, Egypt. FAU - Hassan, Fatma E AU - Hassan FE AUID- ORCID: 0000-0002-3985-8931 AD - Department of Physiology, General Medicine Practice Program, Batterjee Medical College, Jeddah 21442, Saudi Arabia. AD - Medical Physiology Department, Faculty of Medicine, Cairo University, Kasr Alainy, Giza 11562, Egypt. FAU - Ewedah, Tassneim M AU - Ewedah TM AD - Pharmaceutics and Pharmaceutical Technology Department, Faculty of Pharmacy, Egyptian Russian University, Cairo 11829, Egypt. FAU - Mostafa Abd El Galil, Rania AU - Mostafa Abd El Galil R AD - Department of Pharmaceutics, College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science and Technology (MUST), 6th of October City 12566, Egypt. FAU - Nour El-Din, Mahmoud AU - Nour El-Din M AD - Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Sadat City, Menoufia 32897, Egypt. FAU - Ashmawy, Ahmed I AU - Ashmawy AI AUID- ORCID: 0000-0001-7945-6921 AD - Department of Pharmacology and Therapeutics, Faculty of Pharmacy, Pharos University in Alexandria (PUA), Alexandria 21311, Egypt. FAU - Ahmed, Heba Sabry AU - Ahmed HS AD - Department of Clinical Pharmacology, Faculty of Medicine, Zagazig University, Zagazig 44519, Egypt. FAU - Mansy, Azza ElSayed Abd ElFattah AU - Mansy AEAE AD - Department of Clinical Pharmacy, Faculty of Pharmacy, Menoufia University, Menoufia 32811, Egypt. FAU - Hamdan, Ahmed Mohsen Elsaid AU - Hamdan AME AUID- ORCID: 0000-0003-4930-792X AD - Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Tabuk, Tabuk 71491, Saudi Arabia. AD - Prince Fahad bin Sultan Chair for Biomedical Research (PFSCBR), Tabuk 74191, Saudi Arabia. LA - eng PT - Journal Article DEP - 20260930 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708515 OTO - NOTNLM OT - Ehrlich solid carcinoma OT - Ocimum basilicum oil OT - breast cancer OT - cytotoxicity OT - dissolving microneedles OT - nanovesicles OT - neratinib OT - propolis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:43 PHST- 2026/07/31 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - pharmaceutics18101244 [pii] AID - pharmaceutics-18-01244 [pii] AID - 10.3390/pharmaceutics18101244 [doi] PST - epublish SO - Pharmaceutics. 2026 Sep 30;18(10):1244. doi: 10.3390/pharmaceutics18101244. PMID- 42942772 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Sep 29 TI - Drug Repositioning in Kinase Inhibitor Discovery for Cancer Therapy. LID - 10.3390/pharmaceutics18101240 [doi] LID - 1240 AB - Repurposing of existing drugs has emerged as a time- and cost-effective strategy in oncology drug discovery. Given the critical role of dysregulated kinase signaling in cancer, kinase-focused repurposing strategies have attracted considerable interest. This approach encompasses both "target hopping" between different kinases and the discovery of unexpected kinase inhibitory activity in non-kinase drugs. In this review, we summarize recent advances in the repositioning of kinase inhibitors from medicinal chemistry and structural biology perspectives. We highlight the structural basis and binding modes that enable kinase polypharmacology and therapeutic exploitation of off-target activity. Furthermore, we discuss key structural optimization strategies used to convert initial repurposing hits into potent and selective anticancer agents. Overall, kinase-focused repurposing offers a rational, cost-efficient route to expand anticancer options by exploiting conserved kinase-recognition features and refining selectivity through structure-guided optimization. Translation remains constrained by tumor heterogeneity, incomplete target validation, computational-model bias, PK/PD and toxicity limitations, and regulatory and IP barriers. Integrating interpretable AI and multi-omics with kinome-wide profiling, direct target-engagement assays, patient-relevant models, and data sharing should improve patient stratification and accelerate clinical validation. FAU - Sun, Qianran AU - Sun Q AD - Department of Pharmacy, Peking University First Hospital, Beijing 100034, China. AD - State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. FAU - Pang, Xiaocong AU - Pang X AD - Department of Pharmacy, Peking University First Hospital, Beijing 100034, China. AD - State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. FAU - Liu, Wenwu AU - Liu W AUID- ORCID: 0009-0003-7509-2157 AD - Department of Pharmacy, Peking University First Hospital, Beijing 100034, China. AD - State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China. LA - eng GR - 2025YFA0922400/the National Key Research and Development Program of China/ PT - Journal Article PT - Review DEP - 20260929 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708125 OTO - NOTNLM OT - artificial intelligence OT - drug repurposing OT - kinase inhibitor OT - oncology COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/29 CRDT- 2026/10/27 10:43 PHST- 2026/08/19 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/09/29 00:00 [pmc-release] AID - pharmaceutics18101240 [pii] AID - pharmaceutics-18-01240 [pii] AID - 10.3390/pharmaceutics18101240 [doi] PST - epublish SO - Pharmaceutics. 2026 Sep 29;18(10):1240. doi: 10.3390/pharmaceutics18101240. PMID- 42942764 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Sep 29 TI - Pirenzepine Reverses Chemotherapy-Induced Peripheral Neuropathy in Mice. LID - 10.3390/pharmaceutics18101233 [doi] LID - 1233 AB - Background/Objectives: Mitochondrial dysfunction is a common feature of many peripheral neuropathies and agents that enhance mitochondrial function have potential as pan-neuropathy therapeutics. M1 receptor (M1R) antagonists enhance mitochondrial function and reverse multiple indices of neuropathy in rodents and humans with diabetic neuropathy. The present studies extend evaluation of the M1R-selective antagonist pirenzepine to mouse models of chemotherapy-induced peripheral neuropathy (CIPN), with a primary focus on pain relief. Results: Oxaliplatin-exposed mice developed allodynia to light touch that persisted for months before resolution. Systemic pirenzepine (1 mg/kg/day) starting prior to chemotherapy did not prevent allodynia but accelerated recovery. Topical delivery (4% pirenzepine in hydrogel) to the paw, beginning after onset of allodynia, also accelerated recovery, with efficacy persisting weeks after treatment cessation and accompanied by normalization of paw responses to heat. Allodynia in paclitaxel-exposed mice was also reversed, but not prevented, by systemic or topical pirenzepine, while heat hyperalgesia and nerve conduction slowing were dose-dependently normalized. Efficacy of pirenzepine was not associated with disruption of the cytotoxicity of chemotherapeutics, as pirenzepine did not alter the LD50 of paclitaxel when applied to breast or ovarian cancer tumor lines in vitro. Tumor suppression by a cisplatin/paclitaxel cocktail given to mice seeded with OVCAR-8 ovarian cancer cells was also not impacted by pirenzepine. In contrast, pirenzepine did not prevent or reverse allodynia caused by the proteasome inhibitor bortezomib. Conclusions: These studies position pirenzepine as a novel interventional treatment against established neuropathy caused by taxanes and platin-based agents and may guide design of therapeutic regimens for future clinical studies. FAU - Kassahun, Markos AU - Kassahun M AD - Department of Pathology, University of California San Diego, San Diego, CA 92093, USA. FAU - Tayarani, Alireza AU - Tayarani A AUID- ORCID: 0000-0002-8016-9775 AD - Department of Pathology, University of California San Diego, San Diego, CA 92093, USA. FAU - Freedman, Kyle AU - Freedman K AUID- ORCID: 0009-0004-1803-3297 AD - Department of Pathology, University of California San Diego, San Diego, CA 92093, USA. FAU - Gastelum, Sebastian AU - Gastelum S AUID- ORCID: 0009-0009-9037-1086 AD - Department of Pathology, University of California San Diego, San Diego, CA 92093, USA. FAU - Nemmani, Rakesh AU - Nemmani R AD - Department of Pathology, University of California San Diego, San Diego, CA 92093, USA. FAU - Andrew, Jacob AU - Andrew J AD - Department of Ob/Gyn & Reproductive Sciences, University of California San Diego, San Diego, CA 92093, USA. FAU - Soylu, Meryem H AU - Soylu MH AD - Department of Ob/Gyn & Reproductive Sciences, University of California San Diego, San Diego, CA 92093, USA. FAU - Frizzi, Katie E AU - Frizzi KE AD - Department of Pathology, University of California San Diego, San Diego, CA 92093, USA. FAU - Hansen, Angela AU - Hansen A AD - WinSanTor Inc., 7220 Trade St., San Diego, CA 92121, USA. FAU - Fernyhough, Paul AU - Fernyhough P AD - Division of Neurodegenerative and Neurodevelopmental Disorders, St. Boniface Hospital Albrechtsen Research Centre, R4046-351 Tache Ave, Winnipeg, MB R2H 2A6, Canada. AD - Department of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, MB R3E 0T6, Canada. FAU - Jolivalt, Corinne G AU - Jolivalt CG AUID- ORCID: 0000-0003-4391-1341 AD - Department of Pathology, University of California San Diego, San Diego, CA 92093, USA. FAU - Stupack, Dwayne G AU - Stupack DG AUID- ORCID: 0000-0003-4396-5745 AD - Department of Ob/Gyn & Reproductive Sciences, University of California San Diego, San Diego, CA 92093, USA. FAU - Calcutt, Nigel A AU - Calcutt NA AD - Department of Pathology, University of California San Diego, San Diego, CA 92093, USA. LA - eng GR - R42 CA213555/CA/NCI NIH HHS/United States GR - 1R42CA213555-02A1/NH/NIH HHS/United States PT - Journal Article DEP - 20260929 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708127 OTO - NOTNLM OT - CIPN OT - allodynia OT - chemotherapy-induced peripheral neuropathy OT - muscarinic receptor antagonist OT - neuropathic pain OT - peripheral neuropathy OT - pirenzepine COIS- N.A.C. and P.F. are co-founders of Winsantor Inc., which holds IP in this space. A.H. was an employee of WinSanTor at the time that the studies were performed. All the other authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/29 CRDT- 2026/10/27 10:43 PHST- 2026/08/29 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/09/29 00:00 [pmc-release] AID - pharmaceutics18101233 [pii] AID - pharmaceutics-18-01233 [pii] AID - 10.3390/pharmaceutics18101233 [doi] PST - epublish SO - Pharmaceutics. 2026 Sep 29;18(10):1233. doi: 10.3390/pharmaceutics18101233. PMID- 42942761 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Sep 28 TI - Co-Delivery of Fluconazole and Riparin B Using Nanostructured Lipid Carriers: A Quality-by-Design Approach for Enhanced Antifungal Activity. LID - 10.3390/pharmaceutics18101229 [doi] LID - 1229 AB - Background/Objectives: The increasing incidence of invasive fungal infections and the emergence of antifungal resistance have limited the effectiveness of conventional therapies against Candida spp. This study aimed to develop and optimize nanostructured lipid carriers (NLCs) containing fluconazole (FLC) and Riparin B (RIP) using a systematic Quality-by-Design (QbD) approach. Methods: The optimized NLCs were characterized by particle size, polydispersity index (PDI), zeta potential, DSC, and FTIR analyses. Molecular docking was used as a complementary exploratory approach to investigate possible interactions of RIP with representative components of the lipid matrix and with fungal resistance-associated proteins. Release profiles and antifungal activity against Candida spp. were also evaluated. Results: The optimized NLCs exhibited a mean particle size of 144.9 +/- 2.25 nm, PDI of 0.153 +/- 0.03, and zeta potential of -27.8 +/- 1.81 mV. DSC and FTIR analyses supported the structural organization of the lipid matrix and the formation of an imperfect crystalline network, providing structural defects favorable for the incorporation of the active compounds. RIP exhibited more favorable predicted interactions than FLC within the specific lipid configuration evaluated, although these docking scores should not be interpreted as direct thermodynamic measurements of drug-lipid affinity or as quantitative predictors of encapsulation efficiency. Docking analyses with MdR1, CdR1, and CdR2 identified possible interactions of RIP with these resistance-associated proteins, providing a structural hypothesis that may contribute to understanding the potentiation of FLC activity. However, these computational results do not establish direct efflux-pump inhibition. Release profiles followed distinct kinetic regimes, consistent with matrix relaxation for RIP and Fickian diffusion for FLC. Although RIP alone did not exhibit relevant antifungal activity, its association with FLC potentiated FLC activity against Candida spp., while incorporation of the combination into NLCs produced a further enhancement, significantly reducing the minimum inhibitory concentration. Conclusions: These findings indicate that the optimized NLC platform can enhance the antifungal performance of the FLC-RIP combination and provide a formulation framework for further investigation of this co-delivery strategy. FAU - do Nascimento, Matheus Oliveira AU - do Nascimento MO AD - Postgraduate Program in Pharmaceutical Sciences, Dermopharmacy Laboratory, Pharmaceutical Technology Center, Federal University of Piaui, Teresina 64049-550, PI, Brazil. FAU - Dias, Leandro de Sousa AU - Dias LS AUID- ORCID: 0000-0002-9087-0194 AD - Postgraduate Program in Pharmaceutical Sciences, Microbiology Research Laboratory, Department of Parasitology and Microbiology, Federal University of Piaui, Teresina 64049-550, PI, Brazil. FAU - Meneses, Allan Kayk Sales AU - Meneses AKS AUID- ORCID: 0000-0002-2870-7724 AD - Postgraduate Program in Chemistry, Organic Geochemistry Laboratory, Department of Chemistry, Federal University of Piaui, Teresina 64049-550, PI, Brazil. FAU - Lima, Sidney Goncalo de AU - Lima SG AUID- ORCID: 0000-0001-8754-1499 AD - Postgraduate Program in Chemistry, Organic Geochemistry Laboratory, Department of Chemistry, Federal University of Piaui, Teresina 64049-550, PI, Brazil. FAU - Moura, Carla Veronica Rodarte de AU - Moura CVR AD - Postgraduate Program in Chemistry, Biodiesel and Materials Laboratory, Department of Chemistry, Federal University of Piaui, Teresina 64049-550, PI, Brazil. FAU - Gutierrez, Stanley Juan Chavez AU - Gutierrez SJC AD - Undergraduate Course in Pharmacy, Department of Pharmacy, Federal University of Piaui, Teresina 64049-550, PI, Brazil. FAU - Barreto, Humberto Medeiros AU - Barreto HM AUID- ORCID: 0000-0001-5054-7555 AD - Postgraduate Program in Pharmaceutical Sciences, Microbiology Research Laboratory, Department of Parasitology and Microbiology, Federal University of Piaui, Teresina 64049-550, PI, Brazil. FAU - do Nascimento, Denise Andrade AU - do Nascimento DA AD - Department of Physics, Federal University of Roraima (UFRR), Boa Vista 69310-000, RR, Brazil. FAU - Zazeri, Gabriel AU - Zazeri G AUID- ORCID: 0000-0001-9957-9697 AD - Department of Physics, Federal University of Roraima (UFRR), Boa Vista 69310-000, RR, Brazil. FAU - Carvalho, Andre Luis Menezes AU - Carvalho ALM AD - Postgraduate Program in Pharmaceutical Sciences, Dermopharmacy Laboratory, Pharmaceutical Technology Center, Federal University of Piaui, Teresina 64049-550, PI, Brazil. AD - Undergraduate Course in Pharmacy, Department of Pharmacy, Federal University of Piaui, Teresina 64049-550, PI, Brazil. LA - eng GR - 001/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/ GR - 01.24.0609.00-Ref.: 2382/24/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ PT - Journal Article DEP - 20260928 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708304 OTO - NOTNLM OT - fluconazole OT - molecular docking OT - nanostructured lipid carriers OT - quality by design OT - riparin B COIS- The authors declare that there are no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/28 CRDT- 2026/10/27 10:43 PHST- 2026/08/25 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - pharmaceutics18101229 [pii] AID - pharmaceutics-18-01229 [pii] AID - 10.3390/pharmaceutics18101229 [doi] PST - epublish SO - Pharmaceutics. 2026 Sep 28;18(10):1229. doi: 10.3390/pharmaceutics18101229. PMID- 42942759 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Sep 27 TI - Drug Delivery Vehicle-Teriflunomide Interactions Affect Treatment and Fluorine Magnetic Resonance in an Animal Model of Multiple Sclerosis. LID - 10.3390/pharmaceutics18101225 [doi] LID - 1225 AB - Background/Objectives: Teriflunomide (TF) is a fluorinated oral therapy approved for relapsing-remitting multiple sclerosis that can be detected using fluorine-19 ((19)F) magnetic resonance (MR) methods. In preclinical studies, palatable drug delivery vehicles may facilitate voluntary oral administration and reduce procedure-related stress; however, they may also interact with the drug and alter its pharmacological and MR properties. We investigated whether sweetened milk (SM), compared with sucrose-containing carboxymethylcellulose (SCMC), affects the therapeutic efficacy, tissue distribution, and (19)F MR signal of TF in experimental autoimmune encephalomyelitis (EAE), a mouse model of MS. Methods: Twenty-four female SJL/J mice received TF (30 mg/kg/day) in SM or SCMC, or the corresponding vehicle controls, by micropipette-guided drug administration (MDA) from day -2 to day 22. Clinical disease was monitored; hepatic TF was assessed ex vivo by (19)F MR spectroscopy (MRS) and imaging (MRI) at 9.4 T; and brain TF was quantified by HPLC-MS. Formulation-dependent (19)F MR spectral and MR relaxation properties were examined in vitro using phantoms containing TF in different vehicles, including DMSO, SM, SCMC, and serum. Intrinsic pharmacodynamic activity was evaluated by dihydroorotate dehydrogenase (DHODH) inhibition. Results: TF in SCMC reduced EAE severity during peak disease and delayed disease onset, whereas TF in SM produced no sustained therapeutic benefit. Hepatic (19)F MR signals were approximately twofold higher with SCMC than with SM, and brain TF concentrations were 4.8-fold higher. Phantom studies revealed formulation-dependent changes in chemical shift, spectral linewidth, and the MRI relaxation time T(2), including a 65-fold shortening in CMC + SM relative to CMC alone. SM did not reduce the intrinsic DHODH inhibitory potency of TF, indicating that the diminished in vivo efficacy was not attributable to impaired pharmacodynamic activity. Conclusions: As an oral delivery vehicle, sweetened milk altered TF therapeutic efficacy, as well as (19)F MR signal and tissue distribution in the EAE model. Palatable vehicles used for voluntary oral treatment should therefore be carefully selected and validated, as vehicle-drug interactions may alter tissue exposure, confound (19)F MR detection and, more importantly, compromise therapeutic efficacy. FAU - Hu, Xiang AU - Hu X AUID- ORCID: 0009-0007-6992-3056 AD - Berlin Ultrahigh Field Facility, Max-Delbruck-Center for Molecular Medicine in the Helmholtz Association (MDC), 13125 Berlin, Germany. AD - Charite-Universitatsmedizin Berlin, 10117 Berlin, Germany. FAU - Saha, Nandita AU - Saha N AUID- ORCID: 0000-0001-8555-2477 AD - Berlin Ultrahigh Field Facility, Max-Delbruck-Center for Molecular Medicine in the Helmholtz Association (MDC), 13125 Berlin, Germany. AD - Charite-Universitatsmedizin Berlin, 10117 Berlin, Germany. FAU - Chen, Yinhao AU - Chen Y AUID- ORCID: 0009-0005-0789-745X AD - Berlin Ultrahigh Field Facility, Max-Delbruck-Center for Molecular Medicine in the Helmholtz Association (MDC), 13125 Berlin, Germany. AD - Charite-Universitatsmedizin Berlin, 10117 Berlin, Germany. FAU - Millward, Jason M AU - Millward JM AUID- ORCID: 0000-0003-4484-2798 AD - Berlin Ultrahigh Field Facility, Max-Delbruck-Center for Molecular Medicine in the Helmholtz Association (MDC), 13125 Berlin, Germany. AD - Experimental and Clinical Research Center (ECRC), A Joint Cooperation Between the Charite Medical Faculty and the Max-Delbruck Center for Molecular Medicine in the Helmholtz Association, 13125 Berlin, Germany. FAU - Oder, Andreas AU - Oder A AD - Medicinal Chemistry, Leibniz-Forschungsinstitut fur Molekulare Pharmakologie (FMP), 13125 Berlin, Germany. FAU - Mallow, Keven AU - Mallow K AD - Medicinal Chemistry, Leibniz-Forschungsinstitut fur Molekulare Pharmakologie (FMP), 13125 Berlin, Germany. FAU - Munchberg, Stefanie AU - Munchberg S AD - Berlin Ultrahigh Field Facility, Max-Delbruck-Center for Molecular Medicine in the Helmholtz Association (MDC), 13125 Berlin, Germany. FAU - Prinz, Christian AU - Prinz C AUID- ORCID: 0000-0003-3330-031X AD - SRH Fernhochschule-The Mobile University, 88499 Riedlingen, Germany. FAU - Waiczies, Helmar AU - Waiczies H AUID- ORCID: 0000-0001-6651-4790 AD - Tool Time GmbH, 10245 Berlin, Germany. FAU - Rothe, Michael AU - Rothe M AD - Lipidomix GmbH, 13125 Berlin, Germany. FAU - Neuenschwander, Martin AU - Neuenschwander M AUID- ORCID: 0000-0002-3114-7975 AD - Medicinal Chemistry, Leibniz-Forschungsinstitut fur Molekulare Pharmakologie (FMP), 13125 Berlin, Germany. FAU - Nazare, Marc AU - Nazare M AUID- ORCID: 0000-0002-1602-2330 AD - Medicinal Chemistry, Leibniz-Forschungsinstitut fur Molekulare Pharmakologie (FMP), 13125 Berlin, Germany. FAU - Paul, Friedemann AU - Paul F AD - Charite-Universitatsmedizin Berlin, 10117 Berlin, Germany. AD - Experimental and Clinical Research Center (ECRC), A Joint Cooperation Between the Charite Medical Faculty and the Max-Delbruck Center for Molecular Medicine in the Helmholtz Association, 13125 Berlin, Germany. FAU - Niendorf, Thoralf AU - Niendorf T AUID- ORCID: 0000-0001-7584-6527 AD - Berlin Ultrahigh Field Facility, Max-Delbruck-Center for Molecular Medicine in the Helmholtz Association (MDC), 13125 Berlin, Germany. AD - Experimental and Clinical Research Center (ECRC), A Joint Cooperation Between the Charite Medical Faculty and the Max-Delbruck Center for Molecular Medicine in the Helmholtz Association, 13125 Berlin, Germany. FAU - Waiczies, Sonia AU - Waiczies S AUID- ORCID: 0000-0002-9916-9572 AD - Berlin Ultrahigh Field Facility, Max-Delbruck-Center for Molecular Medicine in the Helmholtz Association (MDC), 13125 Berlin, Germany. AD - Experimental and Clinical Research Center (ECRC), A Joint Cooperation Between the Charite Medical Faculty and the Max-Delbruck Center for Molecular Medicine in the Helmholtz Association, 13125 Berlin, Germany. LA - eng GR - G-1563-202.2/2023/German-Israeli Foundation for Scientific Research and Development/ PT - Journal Article DEP - 20260927 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708660 OTO - NOTNLM OT - drug-delivery vehicle OT - experimental autoimmune encephalomyelitis OT - fluorine-19 magnetic resonance imaging OT - fluorine-19 magnetic resonance spectroscopy OT - multiple sclerosis OT - pharmacodynamics OT - teriflunomide OT - tissue distribution OT - voluntary oral administration COIS- Helmar Waiczies was employed by the Tool Time GmbH and Michael Rothe was employed by the Lipidomix GmbH. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/27 CRDT- 2026/10/27 10:43 PHST- 2026/08/11 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - pharmaceutics18101225 [pii] AID - pharmaceutics-18-01225 [pii] AID - 10.3390/pharmaceutics18101225 [doi] PST - epublish SO - Pharmaceutics. 2026 Sep 27;18(10):1225. doi: 10.3390/pharmaceutics18101225. PMID- 42942758 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Sep 27 TI - A Biology-Driven Framework for Selecting, Engineering, and Validating Physiologically Relevant Multicellular Tumour Models. LID - 10.3390/pharmaceutics18101227 [doi] LID - 1227 AB - Physiologically relevant tumour models are increasingly used in cancer research, drug development, and functional precision oncology, yet selection often remains platform-led rather than biology-led. This structured critical narrative review proposes an operational framework linking model selection, engineering, and validation to six interdependent domains: multicellular organization and heterogeneity, extracellular-matrix architecture and mechanics, stromal regulation, vascular and interstitial transport, immune regulation and communication, and metabolic regulation with biochemical gradients. The framework distinguishes mechanism-guided questions, in which a candidate causal mechanism can be prespecified, from discovery-oriented questions, in which the determinant is unknown and candidate functions are tested through nested or factorial configurations before a minimal sufficient explanatory model is defined. Required functions are classified as essential, context-modifying, non-essential, or uncertain, and preservation-oriented, reconstruction-oriented, and hybrid strategies are distinguished from the technological format used to implement them. For pharmaceutical applications, delivered exposure, matrix or device binding, tissue penetration, transport, dosing schedule, and recovery are treated as qualification variables when they can change apparent potency or response. The practical contribution is a traceable biology-to-decision record linking the decision and mechanism status to function selection, model configuration, qualification evidence, complexity-retention rules, and claim boundaries. Model qualification is separated from external association, prospective predictive validity, and clinical utility. Among the representative studies appraised, direct functional and configuration-specific pharmacological evidence was demonstrated more often than interlaboratory transfer, independently validated prospective prediction, or clinical utility. Physiological relevance should therefore be demonstrated through decision-critical function rather than inferred from platform complexity or component count. The proposed decision rules remain unvalidated prospectively and across laboratories. FAU - Wu, Ying-Ta AU - Wu YT AUID- ORCID: 0000-0003-0156-963X AD - MatchCure Inc., 3F., No. 632, Guangfu South Road, Da'an District, Taipei City 10695, Taiwan. FAU - Govindaraju, Darshan T AU - Govindaraju DT AD - MatchCure Inc., 3F., No. 632, Guangfu South Road, Da'an District, Taipei City 10695, Taiwan. FAU - Lakshmikanthacharya, Girija M AU - Lakshmikanthacharya GM AD - Independent Researcher, Zhonghe District, New Taipei City 23561, Taiwan. FAU - Hsu, Tzu-Yu AU - Hsu TY AD - MatchCure Inc., 3F., No. 632, Guangfu South Road, Da'an District, Taipei City 10695, Taiwan. FAU - Lin, Chen-Fang AU - Lin CF AD - MatchCure Inc., 3F., No. 632, Guangfu South Road, Da'an District, Taipei City 10695, Taiwan. FAU - Sharma, Shashwat AU - Sharma S AD - International Ph.D. Program in Medicine, College of Medicine, Taipei Medical University, Taipei City 11031, Taiwan. FAU - Shivanna, Anilkumar T AU - Shivanna AT AUID- ORCID: 0000-0001-9670-7550 AD - MatchCure Inc., 3F., No. 632, Guangfu South Road, Da'an District, Taipei City 10695, Taiwan. LA - eng PT - Journal Article PT - Review DEP - 20260927 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708530 OTO - NOTNLM OT - bioprinting OT - drug delivery OT - drug discovery OT - functional precision oncology OT - multicellular tumour models OT - patient-derived models OT - physiologically relevant tumour models OT - tumour microenvironment OT - tumour organoids OT - tumour-on-chip COIS- A.T.S., D.T.G., Y.-T.W., T.-Y.H. and C.-F.L. are employees of MatchCure Inc. G.M.L. is an Independent Researcher and reports no institutional or commercial affiliation relevant to this review. S.S. is affiliated with the International Ph.D. Program in Medicine, College of Medicine, Taipei Medical University, and reports no commercial affiliation relevant to this review. MatchCure develops proprietary patient-derived tumour-model workflows and provides commercial functional drug-screening services; these activities overlap directly with the subject of this review and constitute a potential commercial conflict of interest. No MatchCure platform or service is included in Section 4.8, Table 3, or Supplementary Tables S5A and S5B. At the time of manuscript preparation, neither the authors nor MatchCure Inc. had a financial, licensing, distribution, consultancy, sponsored-research, data-sharing, or other commercial relationship with any provider listed in Supplementary Tables S5A and S5B. No listed provider was contacted for this review, supplied unpublished information, influenced platform inclusion, or participated in evidence interpretation or manuscript preparation. The eligibility, source-separation, verification, and non-ranking safeguards used to limit commercial-selection bias are described in Section 1.4 and Supplementary Table S3. These safeguards improve transparency but cannot eliminate affiliation-related selection or interpretation bias, which remains an acknowledged limitation of the commercial evidence map. The authors declare no other conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/27 CRDT- 2026/10/27 10:43 PHST- 2026/08/14 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - pharmaceutics18101227 [pii] AID - pharmaceutics-18-01227 [pii] AID - 10.3390/pharmaceutics18101227 [doi] PST - epublish SO - Pharmaceutics. 2026 Sep 27;18(10):1227. doi: 10.3390/pharmaceutics18101227. PMID- 42942757 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Sep 27 TI - Bupivacaine-Loaded PLGA Nanoparticles for Long-Term Management of Chronic Neuropathic Pain. LID - 10.3390/pharmaceutics18101226 [doi] LID - 1226 AB - Background/Objectives: Chronic neuropathic pain imposes a substantial burden on patients and society, and opioids are unsuitable for long-term use due to their addictive potential. To overcome this limitation, we encapsulated bupivacaine in PLGA nanoparticles to achieve sustained-release analgesia with reduced systemic toxicity. Methods: The analgesic efficacy was evaluated in rat models of chronic compression of the dorsal root ganglion (CCD) and chronic constriction injury (CCI). Nanoparticles were characterized using scanning electron microscopy (SEM) for morphology and size, and X-ray diffraction (XRD) for dispersion. In vivo electrophysiological recordings, including action potentials from DRG neuronal somata and compound action potentials in the sciatic nerve, were performed to investigate peripheral analgesic mechanisms, while plasma bupivacaine concentrations were measured by high-performance liquid chromatography (HPLC). Results: Bupivacaine nanoparticles significantly alleviated mechanical pain in both CCD and CCI rats, but had no effect on thermal pain. They selectively inhibited C-fiber conduction without affecting A-fibers. Importantly, plasma bupivacaine levels remained well below the corresponding toxic thresholds, indicating a favorable safety profile. Conclusions: These findings suggest that bupivacaine-loaded PLGA nanoparticles represent a promising long-term therapeutic option for chronic neuropathic pain management. FAU - Wang, Tao AU - Wang T AUID- ORCID: 0000-0001-5521-0644 AD - Department of Human Anatomy, Histology and Embryology, State Key Laboratory of Common Mechanism Research for Major Diseases, Neuroscience Center, Joint Laboratory of Anesthesia and Pain, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking, Union Medical College, Beijing 100005, China. FAU - Tang, Keyun AU - Tang K AUID- ORCID: 0000-0002-5128-2648 AD - Department of Dermatology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, National Clinical Research Center for Dermatologic and Immunologic Diseases, Beijing 100730, China. FAU - Sun, Fengrun AU - Sun F AUID- ORCID: 0000-0002-1512-612X AD - Department of Human Anatomy, Histology and Embryology, State Key Laboratory of Common Mechanism Research for Major Diseases, Neuroscience Center, Joint Laboratory of Anesthesia and Pain, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking, Union Medical College, Beijing 100005, China. FAU - Ma, Yujia AU - Ma Y AD - Department of Human Anatomy, Histology and Embryology, State Key Laboratory of Common Mechanism Research for Major Diseases, Neuroscience Center, Joint Laboratory of Anesthesia and Pain, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking, Union Medical College, Beijing 100005, China. FAU - Fang, Yehong AU - Fang Y AD - Department of Human Anatomy, Histology and Embryology, State Key Laboratory of Common Mechanism Research for Major Diseases, Neuroscience Center, Joint Laboratory of Anesthesia and Pain, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking, Union Medical College, Beijing 100005, China. FAU - Yu, Ning AU - Yu N AD - Department of Human Anatomy, Histology and Embryology, State Key Laboratory of Common Mechanism Research for Major Diseases, Neuroscience Center, Joint Laboratory of Anesthesia and Pain, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking, Union Medical College, Beijing 100005, China. FAU - Liu, Fan AU - Liu F AUID- ORCID: 0000-0002-9481-0797 AD - Department of Human Anatomy, Histology and Embryology, State Key Laboratory of Common Mechanism Research for Major Diseases, Neuroscience Center, Joint Laboratory of Anesthesia and Pain, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking, Union Medical College, Beijing 100005, China. AD - National Human Brain Bank for Development and Function, Beijing 100005, China. FAU - Ma, Chao AU - Ma C AUID- ORCID: 0000-0003-1346-6961 AD - Department of Human Anatomy, Histology and Embryology, State Key Laboratory of Common Mechanism Research for Major Diseases, Neuroscience Center, Joint Laboratory of Anesthesia and Pain, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking, Union Medical College, Beijing 100005, China. AD - National Human Brain Bank for Development and Function, Beijing 100005, China. AD - Chinese Institute for Brain Research, Beijing 102206, China. LA - eng GR - 82271260/National Natural Science Foundation of China/ GR - #2021ZD0201100 Task 1 #2021ZD0201101/STI2030-Major Project/ GR - #2017-I2M-4-005/CAMS Innovation Fund for Medical Sciences/ PT - Journal Article DEP - 20260927 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708572 OTO - NOTNLM OT - CCD OT - CCI OT - bupivacaine OT - mouse OT - nanoparticles OT - neuropathic pain OT - rat COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/27 CRDT- 2026/10/27 10:43 PHST- 2026/07/19 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - pharmaceutics18101226 [pii] AID - pharmaceutics-18-01226 [pii] AID - 10.3390/pharmaceutics18101226 [doi] PST - epublish SO - Pharmaceutics. 2026 Sep 27;18(10):1226. doi: 10.3390/pharmaceutics18101226. PMID- 42942756 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Sep 27 TI - Enhanced Antitumor Effects of 5-Fluorouracil and a Vagal Nerve Blocker Loaded in a Thermosensitive Hydrogel for Localized Drug Delivery in Gastric Cancer. LID - 10.3390/pharmaceutics18101224 [doi] LID - 1224 AB - Background/Objectives: Neural infiltration closely correlates with the progression and poor prognosis of gastrointestinal tumors. The vagus nerve regulates tumor cell proliferation and invasion through acetylcholine (ACh). Combining targeted neurotherapy with chemotherapy has recently emerged as a promising anticancer strategy. Methods: In this study, we designed a biodegradable temperature-sensitive hydrogel (PDLLA-PEG-PDLLA, PLEL) that co-delivers 5-fluorouracil (5-FU) and atropine (a vagal nerve blocker) for local gastric cancer chemotherapy. A key feature of this 5-FU + atropine/PLEL hydrogel system is its sol-gel phase transition in response to physiological temperature, enabling sustained drug release in vivo. Results: After intraperitoneal injection, this dual-drug-loaded hydrogel significantly reduced tumor growth in gastric cancer models, outperforming single-drug-loaded hydrogels and free 5-FU plus atropine combinations. This delivery system also reduced side effects. Conclusions: Therefore, this innovative dual-drug-loaded hydrogel shows strong potential for intratumoral gastric cancer chemotherapy and offers a clinically valuable option for postoperative management. FAU - Wu, Cheng AU - Wu C AD - Department of Gastrointestinal and Hernia Surgery, Ganzhou People's Hospital, Ganzhou 341000, China. FAU - Li, Xunjun AU - Li X AD - Department of General Surgery, Nanfang Hospital, Guangdong Provincial Engineering Technology Research Center of Minimally Invasive Surgery, Southern Medical University, No. 1838, North Guangzhou Avenue, Guangzhou 510515, China. FAU - Liu, Yungeng AU - Liu Y AD - Department of Gastrointestinal and Hernia Surgery, Ganzhou People's Hospital, Ganzhou 341000, China. FAU - Zhong, Wu AU - Zhong W AD - Department of Gastrointestinal and Hernia Surgery, Ganzhou People's Hospital, Ganzhou 341000, China. FAU - Liu, Hongquan AU - Liu H AD - Department of Gastrointestinal and Hernia Surgery, Ganzhou People's Hospital, Ganzhou 341000, China. FAU - Yi, Jianzhong AU - Yi J AD - Department of Gastrointestinal and Hernia Surgery, Ganzhou People's Hospital, Ganzhou 341000, China. FAU - Deng, Shaoxiang AU - Deng S AD - Department of General Surgery, Nanfang Hospital, Guangdong Provincial Engineering Technology Research Center of Minimally Invasive Surgery, Southern Medical University, No. 1838, North Guangzhou Avenue, Guangzhou 510515, China. FAU - Lan, Xiaoliang AU - Lan X AD - Department of General Surgery, Nanfang Hospital, Guangdong Provincial Engineering Technology Research Center of Minimally Invasive Surgery, Southern Medical University, No. 1838, North Guangzhou Avenue, Guangzhou 510515, China. FAU - Fang, Chuanfa AU - Fang C AD - Department of Gastrointestinal and Hernia Surgery, Ganzhou People's Hospital, Ganzhou 341000, China. FAU - Chen, Tao AU - Chen T AUID- ORCID: 0000-0003-1306-6538 AD - Department of Gastrointestinal and Hernia Surgery, Ganzhou People's Hospital, Ganzhou 341000, China. AD - Department of General Surgery, Nanfang Hospital, Guangdong Provincial Engineering Technology Research Center of Minimally Invasive Surgery, Southern Medical University, No. 1838, North Guangzhou Avenue, Guangzhou 510515, China. LA - eng PT - Journal Article DEP - 20260927 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708316 OTO - NOTNLM OT - 5-fluorouracil OT - gastric cancer OT - localized drug delivery OT - thermosensitive hydrogel OT - vagal nerve blocker COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/27 CRDT- 2026/10/27 10:43 PHST- 2026/06/06 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - pharmaceutics18101224 [pii] AID - pharmaceutics-18-01224 [pii] AID - 10.3390/pharmaceutics18101224 [doi] PST - epublish SO - Pharmaceutics. 2026 Sep 27;18(10):1224. doi: 10.3390/pharmaceutics18101224. PMID- 42942750 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Sep 25 TI - Targeting a Moving Threshold: Antigen Density as a Design Constraint for Particulate Drug Delivery in HER2-Low and HER2-Ultralow Breast Cancer-A Narrative Review. LID - 10.3390/pharmaceutics18101218 [doi] LID - 1218 AB - Trastuzumab deruxtecan has made breast tumours with minimal HER2 expression therapeutically actionable, and the descriptors HER2-low and HER2-ultralow now carry regulatory weight. Both were defined by immunohistochemical convention and trial eligibility rather than by calibrated measurement. For a receptor-directed particulate carrier the operative question is not whether HER2 is detectable, but whether enough surface-accessible receptor is present, and suitably distributed, to sustain multivalent attachment, clustering, internalisation and payload transfer. This narrative review asks whether principles established in HER2-overexpressing models, in non-HER2 receptor systems and on synthetic interfaces can defensibly be extrapolated to the HER2-low setting, keeping those evidence classes distinct. Antigen density acts at several stages of the delivery cascade rather than at binding alone, jointly with carrier design and tumour transport. An analytical constraint compounds this: fixed-tissue methods report tissue HER2 abundance but do not selectively quantify the accessible receptor pool a carrier can engage. Within the literature identified, no engagement threshold generalisable across platforms was found; the single measured value belongs to one liposomal system studied in vitro. Lesion-level carrier deposition varied widely in both human imaging datasets identified, which illustrate transport heterogeneity but are not comparable. Targetability is platform-specific, and should be measured rather than inferred from categorical HER2 status. No validated model links receptor abundance, ligand density, avidity and internalisation to biological response, so the framework offered is operational. FAU - Sescu, Vasile Adrian AU - Sescu VA AUID- ORCID: 0009-0002-6336-4883 AD - Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. AD - Department of Obstetrics and Gynecology, Cuza Voda Hospital, 700038 Iasi, Romania. FAU - Filipescu, Alexandru AU - Filipescu A AD - Department of Obstetrics and Gynecology, "Carol Davila" University of Medicine and Pharmacy, 050474 Bucharest, Romania. AD - Department of Obstetrics and Gynecology, Elias University Emergency Hospital, 050474 Bucharest, Romania. FAU - Solomon Condriuc, Iustina Petra AU - Solomon Condriuc IP AD - Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. AD - Department of Obstetrics and Gynecology, Cuza Voda Hospital, 700038 Iasi, Romania. FAU - Burac, Mihaela AU - Burac M AUID- ORCID: 0000-0002-1672-9467 AD - Department of Obstetrics, Gynecology and Human Reproduction, "Nicolae Testemitanu" State University of Medicine and Pharmacy, MD-2004 Chisinau, Moldova. FAU - Scripcariu, Ioana Sadiye AU - Scripcariu IS AD - Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. AD - Department of Obstetrics and Gynecology, Cuza Voda Hospital, 700038 Iasi, Romania. FAU - Socolov, Demetra AU - Socolov D AUID- ORCID: 0000-0002-0979-0200 AD - Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. AD - Department of Obstetrics and Gynecology, Cuza Voda Hospital, 700038 Iasi, Romania. FAU - Socolov, Razvan AU - Socolov R AD - Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. AD - Department of Obstetrics and Gynecology, Clinical Hospital of Obstetrics and Gynecology "Elena Doamna", 700038 Iasi, Romania. FAU - Carauleanu, Alexandru AU - Carauleanu A AUID- ORCID: 0000-0001-9112-7039 AD - Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. AD - Department of Obstetrics and Gynecology, Cuza Voda Hospital, 700038 Iasi, Romania. LA - eng PT - Journal Article PT - Review DEP - 20260925 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708667 OTO - NOTNLM OT - HER2-low breast cancer OT - HER2-ultralow OT - active targeting OT - antigen density OT - avidity OT - multivalency OT - nanomedicine OT - nanoparticle OT - particulate drug delivery OT - receptor density COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/25 CRDT- 2026/10/27 10:43 PHST- 2026/08/29 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - pharmaceutics18101218 [pii] AID - pharmaceutics-18-01218 [pii] AID - 10.3390/pharmaceutics18101218 [doi] PST - epublish SO - Pharmaceutics. 2026 Sep 25;18(10):1218. doi: 10.3390/pharmaceutics18101218. PMID- 42942748 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Sep 24 TI - Efficacy of 5-Aminolevulinic Acid Photodynamic Therapy Versus Observation for Cervical Low-Grade Squamous Intraepithelial Lesions with High-Risk HPV Infection. LID - 10.3390/pharmaceutics18101216 [doi] LID - 1216 AB - Objectives: This study aimed to evaluate the efficacy of photodynamic therapy (PDT) compared to observation in managing low-grade squamous intraepithelial lesions (LSILs) with high-risk HPV infection. Methods: We conducted a retrospective cohort study involving 608 patients with pathologically confirmed LSILs and Hr-HPV infection, divided into PDT or observation groups based on clinical preference and evaluation. PDT was administered as a routine clinical treatment option at our center. Follow-up at 6 months included HPV testing, cytology, and colposcopy. Results: The PDT group exhibited significantly higher regression rates (92.5% vs. 48.3%) and complete HPV clearance rates (80.8% vs. 37.3%) than the observation group. Adverse effects from PDT were mild and transient, with no cervical scarring or skin photosensitivity observed. Ten patients conceived successfully post-PDT, with five uncomplicated deliveries to date. Conclusions: These findings suggest that PDT is an effective, non-invasive treatment for LSILs with Hr-HPV infection, demonstrating favorable regression and safety profiles while preserving cervical integrity, particularly in reproductive-aged women. FAU - Wang, Xiaoyun AU - Wang X AUID- ORCID: 0000-0002-3489-7579 AD - Department of Gynecology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China. FAU - Huang, Jianxia AU - Huang J AD - Department of Gynecology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China. FAU - He, Qiong AU - He Q AD - Department of Gynecology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China. FAU - Zhou, Jianwei AU - Zhou J AD - Department of Gynecology, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China. LA - eng GR - 81301808/National Natural Science Foundation of China/ GR - 82401907/National Natural Science Foundation of China/ PT - Journal Article DEP - 20260924 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708391 OTO - NOTNLM OT - HPV OT - cervical low-grade squamous intraepithelial lesion OT - efficacy OT - observation OT - photodynamic therapy COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:43 PHST- 2026/08/20 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - pharmaceutics18101216 [pii] AID - pharmaceutics-18-01216 [pii] AID - 10.3390/pharmaceutics18101216 [doi] PST - epublish SO - Pharmaceutics. 2026 Sep 24;18(10):1216. doi: 10.3390/pharmaceutics18101216. PMID- 42942740 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Sep 23 TI - Peptide-Based Molecular Glues for Targeted Protein Degradation. LID - 10.3390/pharmaceutics18101208 [doi] LID - 1208 AB - Targeted protein degradation has transformed drug discovery by replacing occupancy-driven inhibition with event-driven removal of disease-causing proteins, opening previously "undruggable" targets, including transcription factors, scaffolding proteins, and intrinsically disordered regions, to therapeutic intervention. Within this landscape, peptide-based molecular glues have emerged as a distinct and underexplored modality, combining the extended, adaptable interaction surfaces of peptides with the mechanistic simplicity of induced-proximity pharmacology. Unlike bifunctional degraders such as PROTACs, which require the concurrent optimization of two linked ligands, peptide molecular glues act through a single engineered interface, stabilizing cooperative ternary complexes between a target protein and an E3 ubiquitin ligase to drive ubiquitination and proteasomal degradation. This review highlights the current understanding of peptide molecular glue biology across five interconnected dimensions: the mechanistic basis of E3 ligase recruitment and cooperative ternary complex formation; rational design strategies spanning sequence selection, structural stabilization, and computational and AI-guided optimization; the structural and biophysical determinants, such as conformational flexibility, interface complementarity, and thermodynamic and kinetic stability, that distinguish productive from non-productive complexes; and emerging therapeutic applications in oncology, hematological malignancy, neurodegeneration, and inflammatory disease, several of which have already reached clinical evaluation. We further examine the comparative advantages of peptide-based architectures, including reduced molecular weight, access to challenging interfaces, and expansion of the addressable E3 ligase repertoire, against persistent translational barriers in proteolytic stability, membrane permeability, immunogenicity, and manufacturability. Taken together, the evidence positions peptide-based molecular glues not as incremental variants of existing degrader platforms, but as a mechanistically distinct therapeutic class capable of substantially widening the druggable proteome. FAU - Aburayan, Walaa S AU - Aburayan WS AUID- ORCID: 0000-0003-3054-1644 AD - Advanced Diagnostics and Therapeutics Institute, Health Sector, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia. FAU - Alradwan, Ibrahim A AU - Alradwan IA AUID- ORCID: 0000-0003-3433-9427 AD - Advanced Diagnostics and Therapeutics Institute, Health Sector, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia. FAU - Alsaffar, Beshair A AU - Alsaffar BA AD - Advanced Diagnostics and Therapeutics Institute, Health Sector, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia. FAU - Alhazzaa, Othman A AU - Alhazzaa OA AD - Healthy Aging Research Institute, Health Sector, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia. FAU - Almughem, Fahad A AU - Almughem FA AUID- ORCID: 0000-0001-9614-7557 AD - Advanced Diagnostics and Therapeutics Institute, Health Sector, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia. FAU - Alshehri, Abdullah A AU - Alshehri AA AUID- ORCID: 0000-0003-0358-4155 AD - Advanced Diagnostics and Therapeutics Institute, Health Sector, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia. FAU - Alduhaymi, Ibtihal S AU - Alduhaymi IS AD - Advanced Diagnostics and Therapeutics Institute, Health Sector, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia. FAU - Fallatah, Mohannad M AU - Fallatah MM AUID- ORCID: 0000-0002-4483-7131 AD - Advanced Diagnostics and Therapeutics Institute, Health Sector, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia. FAU - Alturki, Meshael AU - Alturki M AD - Wellness and Preventative Medicine Institute, Health Sector, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia. FAU - Alshaya, Hassa A AU - Alshaya HA AD - Advanced Diagnostics and Therapeutics Institute, Health Sector, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia. FAU - Tawfik, Essam A AU - Tawfik EA AD - Advanced Diagnostics and Therapeutics Institute, Health Sector, King Abdulaziz City for Science and Technology, Riyadh 11442, Saudi Arabia. LA - eng PT - Journal Article PT - Review DEP - 20260923 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708071 OTO - NOTNLM OT - E3 ubiquitin ligase OT - drug discovery OT - peptide-based molecular glues OT - peptides OT - targeted protein degradation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/23 CRDT- 2026/10/27 10:43 PHST- 2026/08/12 00:00 [received] PHST- 2026/09/08 00:00 [revised] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - pharmaceutics18101208 [pii] AID - pharmaceutics-18-01208 [pii] AID - 10.3390/pharmaceutics18101208 [doi] PST - epublish SO - Pharmaceutics. 2026 Sep 23;18(10):1208. doi: 10.3390/pharmaceutics18101208. PMID- 42942739 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Sep 23 TI - Beyond Creatinine: D-Amino Acids as Emerging Indicators of Kidney Function and Disease. LID - 10.3390/pharmaceutics18101207 [doi] LID - 1207 AB - Despite decades of clinical use, serum creatinine and cystatin C remain imperfect indicators of kidney function, being influenced by muscle mass, age, sex, and inflammation and often showing limited sensitivity to early renal injury. D-amino acids (D-AAs), once largely regarded as by-products of microbial metabolism, have emerged as biologically active chiral metabolites with promising potential for improving the assessment of renal function and disease. This review synthesizes current mechanistic, analytical, and clinical evidence on D-AAs in chronic kidney disease, tracing their origins in diet, gut microbiota, and endogenous racemization through their stereoselective renal transport, tubular handling, and urinary excretion. Among these molecules, D-serine and D-asparagine have emerged as particularly promising biomarkers, showing strong associations with measured glomerular filtration rate and, in early studies, performance comparable or superior to conventional markers, though evidence remains limited to small or selected cohorts, while appearing less dependent on body composition across adult and pediatric populations. Beyond their biomarker potential, D-AAs participate in signaling and metabolic processes linking the gut, kidney, and brain, with altered D-AA homeostasis implicated in both renal injury and uremic neurological dysfunction. We critically examine the biological and clinical confounders, analytical challenges, and validation gaps that currently limit their translation into clinical practice and discuss how chiral metabolomics could contribute to a more precise assessment of kidney function and disease. FAU - Uifalean, Alina AU - Uifalean A AUID- ORCID: 0000-0003-1297-6345 AD - Department of Pharmaceutical Analysis, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, Louis Pasteur Street 6, 400349 Cluj-Napoca, Romania. FAU - Iacobescu, Maria AU - Iacobescu M AUID- ORCID: 0000-0002-0659-8926 AD - Department of Personalized Medicine and Rare Diseases, MEDFUTURE-Institute for Biomedical Research, "Iuliu Hatieganu" University of Medicine and Pharmacy, Louis Pasteur Street 6, 400349 Cluj-Napoca, Romania. FAU - Muresan, Ximena Maria AU - Muresan XM AD - Department of Personalized Medicine and Rare Diseases, MEDFUTURE-Institute for Biomedical Research, "Iuliu Hatieganu" University of Medicine and Pharmacy, Louis Pasteur Street 6, 400349 Cluj-Napoca, Romania. FAU - Heghes, Simona Codruta AU - Heghes SC AUID- ORCID: 0000-0001-7823-4390 AD - Department of Pharmaceutical Analysis, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, Louis Pasteur Street 6, 400349 Cluj-Napoca, Romania. FAU - Salanta, Liana Claudia AU - Salanta LC AUID- ORCID: 0000-0003-2435-405X AD - Department of Food Science, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania. FAU - Moldovan, Radu-Cristian AU - Moldovan RC AUID- ORCID: 0000-0002-2847-1301 AD - Department of Personalized Medicine and Rare Diseases, MEDFUTURE-Institute for Biomedical Research, "Iuliu Hatieganu" University of Medicine and Pharmacy, Louis Pasteur Street 6, 400349 Cluj-Napoca, Romania. FAU - Iuga, Cristina-Adela AU - Iuga CA AUID- ORCID: 0000-0002-0345-0993 AD - Department of Pharmaceutical Analysis, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, Louis Pasteur Street 6, 400349 Cluj-Napoca, Romania. AD - Department of Personalized Medicine and Rare Diseases, MEDFUTURE-Institute for Biomedical Research, "Iuliu Hatieganu" University of Medicine and Pharmacy, Louis Pasteur Street 6, 400349 Cluj-Napoca, Romania. LA - eng GR - 32154/4/16.12.2024/Iuliu Hatieganu University of Medicine and Pharmacy/ PT - Journal Article PT - Review DEP - 20260923 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708084 OTO - NOTNLM OT - D-amino acids OT - D-asparagine OT - D-serine OT - biomarkers OT - chiral metabolomics OT - chronic kidney disease OT - gut-kidney axis COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/23 CRDT- 2026/10/27 10:43 PHST- 2026/08/25 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - pharmaceutics18101207 [pii] AID - pharmaceutics-18-01207 [pii] AID - 10.3390/pharmaceutics18101207 [doi] PST - epublish SO - Pharmaceutics. 2026 Sep 23;18(10):1207. doi: 10.3390/pharmaceutics18101207. PMID- 42942732 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Sep 22 TI - Pharmacokinetic Studies of Triazolyl-Oxazolidinone Derivatives with Anticonvulsant Effects in Rats. LID - 10.3390/pharmaceutics18101201 [doi] LID - 1201 AB - Background/Objectives: Five triazolyl-oxazolidinone derivatives were reported to suppress seizures in rats with different efficacies and durations of action, which may be due to different pharmacokinetic behaviors. This study sought to investigate the pharmacokinetic characteristics of the five selected triazolyl-oxazolidinone derivatives and to correlate these with their anti-seizure activity in rats. A secondary objective was to identify lead compound(s) in this small series for further modification and subsequent development into drug candidates. Methods: Following intraperitoneal injection, the concentrations of the five selected compounds PH066, PH139, PH162, PH166, and PH192, in plasma and brain tissues were measured using LC/MS/MS methods. The brain tissue concentrations for each derivative were subsequently correlated with its observed anti-seizure activity in male Sprague Dawley rats against electrically induced (6 Hz and MES) and chemically-induced (pentylenetetrazole) seizure models. The area under the concentration-time curves in plasma and brain tissues was determined to estimate the average brain distribution of each compound. Anti-seizure activity was quantified using a standardized behavioral scoring system based on the rat's response to seizure-inducing stimuli, with scores assigned by two independent observers. Results: Plasma and brain tissue concentrations were determined over the duration of each experiment. The mean maximum plasma concentrations were 14.6, 9.20, 14.8, 18.2, and 9.00 mug/mL, while the mean maximum brain tissue concentrations were 2.68, 0.14, 1.94, 2.80, and 0.86 mug/g for PH066, PH139, PH162, PH166, and PH192, respectively. The brain to plasma distribution ratios were in the range of 0.15-0.20 for PH066, PH162, and PH166, but were much lower for PH139 and PH192. The brain tissue concentration for each compound was correlated with its anti-seizure activity. The decreasing order of anti-seizure efficacy against the 6 Hz model was PH162 > PH166 > PH066 > PH139 > PH192, which correlated well with their plasma and brain concentrations. The decreasing order of protection against the MES model was PH139 > PH192 > PH162 > PH066 > PH166, while the decreasing order of protection against the pentylenetetrazole model was PH162 approximately PH066 > PH166 approximately PH192 > PH139. Conclusions: Compounds PH066, PH162 and PH166 achieved relatively high plasma and brain tissue concentrations with good plasma to brain distribution ratios. Therefore, they have favorable pharmacokinetic characteristics when compared with compounds PH139 and PH192. Of all the compounds evaluated, PH162 had the best pharmacokinetic characteristics, with consistent moderate to high protection against all the tested seizure models and with sustained anti-seizure activity. Thus, from these studies the order of anti-seizure efficacy and duration of action is PH162 > PH139 > PH166 approximately PH066 > PH192. Thus, PH162 emerged as the potential lead compound when both pharmacokinetic and pharmacodynamic characteristics are considered. FAU - Hedaya, Mohsen A AU - Hedaya MA AUID- ORCID: 0000-0001-6361-9669 AD - Department of Pharmaceutics, College of Pharmacy, Kuwait University, Safat 13060, Kuwait. FAU - Qaddoumi, Mohammad G AU - Qaddoumi MG AD - Department of Pharmacology and Therapeutics, College of Pharmacy, Kuwait University, Safat 13060, Kuwait. FAU - Thomas, Vidhya AU - Thomas V AD - Department of Pharmaceutics, College of Pharmacy, Kuwait University, Safat 13060, Kuwait. FAU - Al-Tannak, Naser F AU - Al-Tannak NF AUID- ORCID: 0000-0002-8901-4032 AD - Department of Pharmaceutical Chemistry, College of Pharmacy, Kuwait University, Safat 13060, Kuwait. FAU - Kombian, Samuel B AU - Kombian SB AUID- ORCID: 0000-0001-9008-2799 AD - Department of Pharmacology & Toxicology, School of Pharmacy and Pharmaceutical Sciences, University for Development Studies, Tamale NT-0272-1946, Ghana. FAU - Phillips, Oludotun A AU - Phillips OA AUID- ORCID: 0000-0002-8350-7338 AD - Department of Pharmaceutical Chemistry, College of Pharmacy, Kuwait University, Safat 13060, Kuwait. LA - eng GR - Research Sector # PP02/19/Kuwait University/ GR - Research Sector Projects Unit (RSPU) grants GS01/01, GS01/03 and GS01/05/Kuwait University/ PT - Journal Article DEP - 20260922 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708525 OTO - NOTNLM OT - anticonvulsant OT - pharmacodynamics OT - pharmacokinetics OT - preclinical OT - triazolyl-oxazolidinone derivatives COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:43 PHST- 2026/08/02 00:00 [received] PHST- 2026/09/10 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - pharmaceutics18101201 [pii] AID - pharmaceutics-18-01201 [pii] AID - 10.3390/pharmaceutics18101201 [doi] PST - epublish SO - Pharmaceutics. 2026 Sep 22;18(10):1201. doi: 10.3390/pharmaceutics18101201. PMID- 42942729 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1999-4923 (Print) IS - 1999-4923 (Electronic) IS - 1999-4923 (Linking) VI - 18 IP - 10 DP - 2026 Sep 22 TI - Radiolabelled Antisense miRNA Oligonucleotides for Molecular Imaging and Therapeutic Applications. LID - 10.3390/pharmaceutics18101197 [doi] LID - 1197 AB - Background/Objectives: MicroRNAs (miRNAs) are small non-coding RNAs (20-24 nucleotides) that regulate post-transcriptional gene expression and are involved in key biological processes, including proliferation and apoptosis. Dysregulation of miRNA expression is associated with numerous diseases, particularly cancer, making them attractive biomarkers and therapeutic targets for precision medicine. Antisense miRNA oligonucleotides (AMOs) have emerged as promising agents for selectively inhibiting miRNA activity. This review examines the development of radiolabelled AMOs targeting miRNAs for diagnostic and imaging applications, with particular attention to their radiolabelling, biological performance, delivery strategies, and potential therapeutic extension. Methods: The literature was examined with a focus on studies describing the synthesis of radiolabelled AMOs targeting miRNAs. Other oligonucleotide-based molecules, such as antisense oligonucleotides (ASOs) and peptide nucleic acids (PNAs), were not considered. Results: Current evidence demonstrates that AMOs can be efficiently radiolabelled with clinically relevant radionuclides, including (99m)Tc and (68)Ga, using bifunctional chelating agents (BFCA) such as MAG3, DOTA, and NOTA while preserving their biological properties. These probes enable noninvasive imaging of their in vivo localization in regions of interest, supporting their potential for precision diagnostics. However, available studies remain limited, and significant challenges persist, particularly regarding targeted delivery, pharmacokinetics, and nonspecific uptake in excretory organs. Therapeutic applications remain at an early preclinical stage, with radioiodinated AMO-21 providing preliminary evidence for therapeutic use. Conclusions: Radiolabelled AMOs represent a potential approach for miRNA targeted molecular imaging, but their clinical translation remains challenging. Future studies should integrate optimized chemical modifications and delivery systems with rigorous in vivo validation and appropriate controls. FAU - Bonanno, Chiara AU - Bonanno C AUID- ORCID: 0009-0007-8977-0843 AD - Department of Chemical Sciences, University of Catania, 95125 Catania, Italy. AD - Institute of Bioimaging and Complex Biological Systems, National Research Council, 90015 Cefalu, Italy. FAU - Romeo, Giuseppe AU - Romeo G AUID- ORCID: 0000-0003-2160-4164 AD - Department of Drug and Health Sciences, University of Catania, 95125 Catania, Italy. AD - NANOMED-Research Center on Nanomedicine and Pharmaceutical Nanotechnology, University of Catania, 95125 Catania, Italy. LA - eng PT - Journal Article PT - Review DEP - 20260922 PL - Switzerland TA - Pharmaceutics JT - Pharmaceutics JID - 101534003 PMC - PMC13708178 OTO - NOTNLM OT - antisense oligonucleotides OT - microRNA OT - molecular imaging OT - radiolabelling COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:43 PHST- 2026/07/02 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - pharmaceutics18101197 [pii] AID - pharmaceutics-18-01197 [pii] AID - 10.3390/pharmaceutics18101197 [doi] PST - epublish SO - Pharmaceutics. 2026 Sep 22;18(10):1197. doi: 10.3390/pharmaceutics18101197. PMID- 42942727 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 21 TI - Tumor Microenvironment-Triggered Dual-Prodrug Nanoplatform for Augmented Photodynamic Therapy: Self-Quenched Photosensitizer Activation and Nitric Oxide-Mediated Sensitization. LID - 10.3390/polym18202606 [doi] LID - 2606 AB - Photodynamic therapy (PDT) holds great promise for cancer treatment owing to its spatiotemporal selectivity and minimal invasiveness. However, its therapeutic efficacy is hampered by photosensitizer self-quenching and intrinsic tumor hypoxia. Herein, we construct a tumor-microenvironment (TME)-activated dual-prodrug nanosystem (PheoA-CSSE-JSK) integrating the photosensitizer prodrug PheoA and nitric-oxide (NO) prodrug JSK for targeted tumor delivery. Tumor-abundant glutathione (GSH) cleaves disulfide bonds to restore the photoactivity of PheoA, which generates reactive oxygen species (ROS) under near-infrared irradiation. Meanwhile, intracellular GSH/glutathione S-transferase (GST) triggers NO release from JSK. NO-derived reactive nitrogen species (RNS) can degrade the tumor collagen matrix, which is inferred to potentially relieve hypoxia and further boost ROS production. GSH consumption, together with NO-mediated inhibition of the mitochondrial respiratory chain, may further augment ROS levels. We propose a self-reinforcing ROS-amplification working model involving PheoA photoactivation, potential NO-associated hypoxia alleviation, GSH depletion, and mitochondrial respiratory-chain inhibition. Collectively, these combined effects facilitate mitochondrial-dependent apoptosis and prominent antitumor outcomes. This TME-responsive dual-prodrug nanoplatform provides a promising strategy to augment PDT against hypoxic tumors. FAU - Gu, Yuxuan AU - Gu Y AD - Hebei Key Laboratory of Functional Polymers, School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300401, China. FAU - Ding, Jiaqi AU - Ding J AD - Hebei Key Laboratory of Functional Polymers, School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300401, China. FAU - Zhang, Jialin AU - Zhang J AD - Hebei Key Laboratory of Functional Polymers, School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300401, China. FAU - Liu, Xiaoxuan AU - Liu X AD - Hebei Key Laboratory of Functional Polymers, School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300401, China. FAU - Xu, Zhuotao AU - Xu Z AD - Hebei Key Laboratory of Functional Polymers, School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300401, China. FAU - Zhang, Jimin AU - Zhang J AUID- ORCID: 0000-0001-7037-5842 AD - Hebei Key Laboratory of Functional Polymers, School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300401, China. FAU - Qu, Xiongwei AU - Qu X AD - Hebei Key Laboratory of Functional Polymers, School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin 300401, China. LA - eng GR - No. 82302391/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709318 OTO - NOTNLM OT - NO-mediated hypoxia alleviation OT - dual-prodrug nano-assembly OT - photodynamic therapy OT - photosensitizer activation OT - self-reinforcing ROS-cascade amplification COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:43 PHST- 2026/09/30 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - polym18202606 [pii] AID - polymers-18-02606 [pii] AID - 10.3390/polym18202606 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 21;18(20):2606. doi: 10.3390/polym18202606. PMID- 42942722 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 21 TI - Polymer-Bound and Macromolecular Hindered Phenolic Antioxidants: Architectures, Anti-Migration Mechanisms, and Applications. LID - 10.3390/polym18202601 [doi] LID - 2601 AB - Hindered phenolic antioxidants are the primary defense against thermo-oxidative degradation in most commercial polymers, yet in many long-lived service environments, their effectiveness is limited less by radical-scavenging chemistry than by physical loss-migration into contact media, extraction by service fluids, and volatilization during processing. This review examines polymer-bound and macromolecular hindered phenols, in which the phenolic function is grafted onto preformed polymers, incorporated by copolymerization, multiplied in dendritic and hyperbranched architectures, or carried by hybrid and bio-based scaffolds. Each architecture family is analyzed against common criteria-structural control, phenolic hydroxyl content and retained activity, compatibility, and scalability-with study-level quantitative analysis of representative studies, selected for the completeness of their quantitative retention and performance data. Anti-migration mechanisms and the methods used to verify retention are critically assessed; in particular, survival of antioxidant activity after extraction is treated as evidence of functional retention, not as proof of covalent attachment, which requires independent structural characterization. Application performance is then reviewed across packaging, infrastructure, electrical insulation, elastomers, engineering plastics, biomedical polyethylene, and recycling. The strongest unmet needs are methodological: standardized extraction-based verification of covalent binding, quantification of phenol utilization efficiency in solid hosts, and long-term validation under sector-relevant protocols. FAU - Jian, Yukun AU - Jian Y AUID- ORCID: 0009-0005-8723-1067 AD - College of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310032, China. AD - School of Chemical Engineering, Ningbo Polytechnic University, Ningbo 315800, China. FAU - Xu, Jiajun AU - Xu J AD - School of Chemical Engineering, Ningbo Polytechnic University, Ningbo 315800, China. LA - eng GR - FG2025045/Department of Education of Zhejiang Province/ PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709062 OTO - NOTNLM OT - anti-migration OT - hindered phenol OT - hyperbranched polymer OT - macromolecular antioxidant OT - polymer recycling OT - polymer-bound stabilizer OT - polyolefin stabilization COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:43 PHST- 2026/09/27 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - polym18202601 [pii] AID - polymers-18-02601 [pii] AID - 10.3390/polym18202601 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 21;18(20):2601. doi: 10.3390/polym18202601. PMID- 42942721 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 21 TI - Effects of Natural Ageing on the Dimensional, Mechanical, and Thermal Properties of Diving Neoprene. LID - 10.3390/polym18202600 [doi] LID - 2600 AB - This study examines the effects of natural ageing on thickness, flexibility, elongation, and thermal resistance of neoprene used in wetsuits. A natural ageing protocol was developed to simulate recreational diving conditions under controlled exposure. Thirty-three neoprene specimens representing 11 material types and three thickness categories were repeatedly immersed in seawater at 15 m depth for 45 min per cycle. Fifty immersion, cleaning, and drying cycles were performed, corresponding to 37.5 h of underwater exposure. Thickness decreased in the majority of samples (p = 0.00043; Cohen's d = 0.64), while pre- and post-ageing values remained very strongly correlated (r = 0.9987). Flexibility decreased significantly (p = 0.000084; Cohen's d = -0.74). Thermal resistance declined significantly (p = 0.00029; Cohen's d = 0.66), with greater losses observed in high-performance neoprene. Elongation showed the greatest ageing-related change, decreasing by approximately 25% (p < 0.000001; Cohen's d = 1.32). Thermal resistance was strongly associated with neoprene thickness, whereas thermal-resistance loss was not correlated with thickness reduction (r = -0.085). Overall, natural ageing affected dimensional, mechanical, and thermal properties through multiple degradation processes, highlighting the importance of long-term performance stability in neoprene material selection and wetsuit design. FAU - Potocic Matkovic, Vesna Marija AU - Potocic Matkovic VM AUID- ORCID: 0000-0002-2907-7306 AD - University of Zagreb Faculty of Textile Technology, 10000 Zagreb, Croatia. FAU - Salopek Cubric, Ivana AU - Salopek Cubric I AUID- ORCID: 0000-0003-4295-5190 AD - University of Zagreb Faculty of Textile Technology, 10000 Zagreb, Croatia. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709272 OTO - NOTNLM OT - elongation OT - flexibility OT - natural ageing OT - neoprene OT - thermal resistance OT - wetsuit performance COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:43 PHST- 2026/09/13 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - polym18202600 [pii] AID - polymers-18-02600 [pii] AID - 10.3390/polym18202600 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 21;18(20):2600. doi: 10.3390/polym18202600. PMID- 42942720 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 21 TI - Effect of Polymer Matrix Type and Particle-Size Distribution on the Mechanical, Microstructural, and Thermal Behavior of Waste Tire Rubber Composites. LID - 10.3390/polym18202599 [doi] LID - 2599 AB - Recycling ground tire rubber (GTR) into polymer-matrix composites requires accounting for both matrix formulation and rubber particle-size distribution. Here, polyurethane, epoxy, novolac-hexamine, and unsaturated polyester systems were compared using four gradations of the same rubber source (16 formulations). A common main-resin-to-rubber mass ratio of 20% was used; differences in required auxiliary components and curing conditions were explicitly considered. The 28-day reference compression results ranged from 1.22 to 1.35 MPa for the polyester formulations to 8.21 MPa for epoxy-K1; epoxy-K1 also reached 2.21 MPa in flexure, whereas polyurethane-K4 reached 6.29 MPa in compression and 1.94 MPa in flexure. Thirteen available compression curves were reprocessed using the 70 x 50 mm loaded face and 70 mm loading length; derived deformation and work-density parameters are apparent, machine-displacement-based measures. Binder-specific UPV trends were exploratory (four gradations per binder). Selected SEM/EDS, ATR-FTIR, and representative TGA/DTG data supplied complementary evidence without establishing interfacial chemical bonding, cure inhibition, or fire performance. The results indicate formulation-dependent strength-deformation trade-offs within the tested materials rather than a universal optimum matrix or gradation. FAU - Mohabbi, Mehrzad AU - Mohabbi M AUID- ORCID: 0000-0001-8584-1658 AD - Department of Civil Engineering, Faculty of Engineering, Bingol University, 12000 Bingol, Turkiye. FAU - Sahin, Canan Kaya AU - Sahin CK AD - Department of Civil Engineering, Faculty of Engineering, Bingol University, 12000 Bingol, Turkiye. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13708796 OTO - NOTNLM OT - epoxy OT - novolac-hexamine OT - particle-size distribution OT - polymer composites OT - polyurethane OT - structure-property relationships OT - unsaturated polyester OT - waste tire rubber COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:43 PHST- 2026/09/25 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - polym18202599 [pii] AID - polymers-18-02599 [pii] AID - 10.3390/polym18202599 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 21;18(20):2599. doi: 10.3390/polym18202599. PMID- 42942718 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 21 TI - Physicochemical Characterization, Binder Rheology, and Engineering Performance of Diatomite-Microfiber Hybrid Asphalt Systems. LID - 10.3390/polym18202597 [doi] LID - 2597 AB - Diatomite and microfibers have been investigated as asphalt modifiers, but limited attention has been given to how differences between diatomite sources and microfiber formulations are reflected across binder- and mixture-scale performance. This study investigated two diatomites (D1 and D2) and three microfibers-polyacrylonitrile (PAN), polyethylene terephthalate (PET), and basalt-using a multiscale experimental approach. The diatomites were characterized by particle-size analysis, SEM-EDS, XRF, and XRD, while the fibers were examined using SEM-EDS, XRD, and binder-retention testing. Diatomite-modified binders containing 10% and 15% modifier were evaluated for conventional, rheological, and aging properties. At the mixture scale, selected 15%D1 and 10%D2 systems were combined with the three fibers and compared with unmodified and 5% SBS-modified references. D1 was finer and had a slightly higher SiO(2) content than D2, while both showed silica-rich porous structures. Diatomite generally increased binder consistency and viscosity and improved the high-temperature rheological response without changing the BBR critical temperature. The 10%D2-PAN mixture achieved the highest indirect tensile strength (4.29 MPa), while the tensile strength ratios of 10%D2-PAN and 10%D2-PET were 82-83%. The 10%D2 formulations showed Cantabro losses of 5.5-6.7% and permanent deformations of 0.247-0.255 mm. In the adapted 100 mm SCB test, 10%D2-PAN also produced the highest flexibility index (10.6) and fracture energy (7.3 kJ/m(2)). Overall, the results indicate that diatomite source characteristics and microfiber formulation should be considered together, with 10%D2-PAN exhibiting a favorable combination of strength, moisture-resistance, particle-loss, permanent-deformation, and fracture-related responses under the investigated conditions. FAU - Aslan, Mustafa Taha AU - Aslan MT AUID- ORCID: 0000-0002-0453-788X AD - Graduate School of Natural and Applied Sciences, Karadeniz Technical University, Trabzon 61080, Turkiye. FAU - Iskender, Erol AU - Iskender E AUID- ORCID: 0000-0001-7934-839X AD - Department of Civil Engineering, Faculty of Technology, Karadeniz Technical University, Trabzon 61830, Turkiye. LA - eng GR - 125M152/The Scientific and Technological Research Council of Turkiye/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13708819 OTO - NOTNLM OT - binder rheology OT - diatomite OT - fracture performance OT - hybrid asphalt modification OT - microfiber reinforcement OT - physicochemical characterization COIS- Mustafa Taha Aslan reports financial support from the Scientific and Technological Research Council of Turkiye (TUBITAK). M.T.A. was formerly employed by Bentas Bentonit, which supplied the diatomite materials used in this study. M.T.A. is no longer employed by the company. Bentas Bentonit had no role in the study design, data collection, analysis, interpretation of the results, preparation of the manuscript, or the decision to submit the manuscript for publication. The other author declares no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:43 PHST- 2026/09/24 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - polym18202597 [pii] AID - polymers-18-02597 [pii] AID - 10.3390/polym18202597 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 21;18(20):2597. doi: 10.3390/polym18202597. PMID- 42942714 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 21 TI - Film Recovery Constraints on Optical and Gravimetric Assessment of Pulsed Gas-Solid Cleaning of Model Plastic Films. LID - 10.3390/polym18202593 [doi] LID - 2593 AB - Cleaning-induced mass changes depend on which films enter the post-cleaning weighing. This study quantifies that dependence using 84 model film runs in an established pulsed gas-solid apparatus and a laboratory stirred water reference. The count-matched weighing criterion retained 26 runs (31.0%): 24.0% of dry cleaning runs and 88.9% of stirred water reference runs. Ten of 21 groups had no eligible gravimetric observation. In one incomplete recovery run, assigning the mean initial clean film mass to the unweighed piece gave a scenario contribution of 19.5 percentage points of the unrestricted gravimetric index, illustrating potential bias without correcting the observation. Optical normalization denominators ranged from 68.0 to 76.2 percentage points; separately selected films introduced a conditional sampling uncertainty component of 0.33-0.99 percentage points in optical recovery. At 120 s, the nominal 5 g polyethylene (PE) configuration had 15.2 percentage point lower mean optical recovery than the 3 g configuration despite similar film count recovery. Reporting recovery-dependent coverage alongside each endpoint keeps laboratory comparisons tied to the film populations actually measured. FAU - Yu, Chaojie AU - Yu C AD - College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. FAU - Xu, Junqing AU - Xu J AD - College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. FAU - Hu, Xichao AU - Hu X AD - Shanghai Tianqiang Environmental Technology Co., Ltd., Shanghai 201415, China. FAU - Zhu, Haochen AU - Zhu H AUID- ORCID: 0000-0003-3805-1842 AD - College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. FAU - Li, Guangming AU - Li G AUID- ORCID: 0000-0002-0281-8258 AD - College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. LA - eng GR - Shanghai Science and Technology Innovation Action Plan (22dz1209000)/Science and Technology Commission of Shanghai Municipality/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13708878 OTO - NOTNLM OT - apparent gravimetric change OT - film count recovery OT - gas-solid fluidization OT - measurement validity OT - optical recovery OT - plastic film cleaning COIS- X.H. is a shareholder of Shanghai Tianqiang Environmental Technology Co., Ltd. The company provided engineering records, and its participation in research analysis and interpretation of the results was through X.H. The authors declare no other conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:43 PHST- 2026/09/23 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - polym18202593 [pii] AID - polymers-18-02593 [pii] AID - 10.3390/polym18202593 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 21;18(20):2593. doi: 10.3390/polym18202593. PMID- 42942705 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 20 TI - Integration of Aerogel Cryogenic Insulation with Carbon Fiber Composites for Hydrogen Storage. LID - 10.3390/polym18202584 [doi] LID - 2584 AB - Aerogels, characterized by their low density and high porosity, possess excellent thermal insulation properties. It is worth exploring whether the materials obtained by combining aerogels and carbon fibers can achieve a better thermal insulation effect for the application of liquid hydrogen storage tanks. The focus of this thesis consists of selecting the vacuum bagging method for the combination of composite laminates and aerogel blankets and evaluating the mechanical properties of the obtained specimens through T-peel experiments and microscopy observations. The aerogel-bond specimen has an average adhesive fracture toughness of 230 J/m(2), in contrast to the polymer-bond specimen's 660 J/m(2). Consequently, the mechanical properties of the polymer-bond specimens surpass those of the aerogel-bond specimens. In this work, thermal conductivity is used to characterize the thermal insulation properties of the materials. The thermal conductivity of the composite specimens is measured by the heat flux meter method and their thermal insulation properties are evaluated. According to the results, the integration of M78.1 and Cryogel-Z has a thermal conductivity of 0.0262 W/m.k, at 50 degrees C. And its thermal conductivity in a 75 degrees C environment is 0.0278 W/m.k. This suggests that it also has excellent thermal insulation properties. Additionally, the simulation results are in agreement with the experimental results. FAU - Fang, Kaituo AU - Fang K AD - College of Electromechanical Engineering, Suqian University, Suqian 223800, China. AD - College of Energy and Power Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China. AD - Jiangsu Engineering Research Center of Key Technology for Intelligent Manufacturing Equipment, Suqian 223800, China. AD - Suqian Key Laboratory of Intelligent Manufacturing, Suqian 223800, China. FAU - Yuan, Shuangyan AU - Yuan S AD - College of Mechanical and Electrical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, China. FAU - Xue, Junxiu AU - Xue J AD - College of Electromechanical Engineering, Suqian University, Suqian 223800, China. AD - Jiangsu Engineering Research Center of Key Technology for Intelligent Manufacturing Equipment, Suqian 223800, China. AD - Suqian Key Laboratory of Intelligent Manufacturing, Suqian 223800, China. FAU - Xu, Chunming AU - Xu C AD - College of Electromechanical Engineering, Suqian University, Suqian 223800, China. AD - Jiangsu Engineering Research Center of Key Technology for Intelligent Manufacturing Equipment, Suqian 223800, China. AD - Suqian Key Laboratory of Intelligent Manufacturing, Suqian 223800, China. FAU - Wang, Yu AU - Wang Y AD - College of Electromechanical Engineering, Suqian University, Suqian 223800, China. AD - Jiangsu Engineering Research Center of Key Technology for Intelligent Manufacturing Equipment, Suqian 223800, China. AD - Suqian Key Laboratory of Intelligent Manufacturing, Suqian 223800, China. FAU - Zhang, Yongming AU - Zhang Y AUID- ORCID: 0000-0001-6127-0096 AD - College of Electromechanical Engineering, Suqian University, Suqian 223800, China. AD - Jiangsu Engineering Research Center of Key Technology for Intelligent Manufacturing Equipment, Suqian 223800, China. AD - Suqian Key Laboratory of Intelligent Manufacturing, Suqian 223800, China. FAU - Tang, Youliang AU - Tang Y AD - College of Electromechanical Engineering, Suqian University, Suqian 223800, China. AD - Jiangsu Engineering Research Center of Key Technology for Intelligent Manufacturing Equipment, Suqian 223800, China. AD - Suqian Key Laboratory of Intelligent Manufacturing, Suqian 223800, China. LA - eng GR - Su Teacher Letter [2024] No.14)/Excellent Teaching Team of Jiangsu Qing Lan Project/ GR - No. K202445/Suqian Sci&Tech Program/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709049 OTO - NOTNLM OT - aerogel OT - carbon fiber composites OT - cryogenic insulation OT - hydrogen storage OT - thermal conductivity COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:43 PHST- 2026/08/16 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - polym18202584 [pii] AID - polymers-18-02584 [pii] AID - 10.3390/polym18202584 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 20;18(20):2584. doi: 10.3390/polym18202584. PMID- 42942698 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 19 TI - Fluorescent Polymer Platforms in Tumour Mechanobiology and Therapy Monitoring. LID - 10.3390/polym18202578 [doi] LID - 2578 AB - Fluorescent polymer platforms reveal different aspects of tumour mechanobiology, but the strength of the evidence behind their optical readouts varies. This narrative review distinguishes calibrated physical measurements, mechanically active therapies assessed by independent assays, and biochemical or delivery readouts. We classify polymer roles as emission generation, mechanical coupling, chemical gating, and carriage or localisation. Among the selected studies, polymer-assisted molecular rotors measured local microviscosity in mouse CT26 tumours, while fluorescent polyacrylamide inclusions enabled intravital solid-stress inference from calibrated deformation. PEG-gold tethers measured receptor tension in cancer-cell culture. Mechanically responsive fluorophores in synthetic networks remain enabling technologies without tumour validation. Our quantitative comparison covers formulation properties, optical operating conditions, calibration, and demonstrated biological settings, labelling unreported values and inapplicable metrics separately. A cholesterol-modifying polyrotaxane illustrates therapy supported by independent cellular mechanics assays; its fluorescence tracks distribution. Protease, pH, oxygen, and transcript probes provide complementary microenvironment or treatment-response information without directly measuring tissue stiffness. The evidence reviewed here establishes neither a clinically validated polymer-fluorescence biomarker of tumour mechanics nor a universal cross-platform performance ranking. Progress requires a defined physical variable and compartment, maintained formulation integrity, and evidence that the measured change informs a functional treatment outcome. FAU - Chen, Xiang AU - Chen X AD - Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310018, China. FAU - Li, Yujie AU - Li Y AUID- ORCID: 0009-0006-4757-234X AD - Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310018, China. FAU - Chen, Jiayi AU - Chen J AD - Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310018, China. FAU - Liu, Wei AU - Liu W AD - Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310018, China. FAU - Zhao, Shengsheng AU - Zhao S AD - Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310018, China. FAU - Jiang, Mengting AU - Jiang M AD - Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310018, China. FAU - Yang, Jing AU - Yang J AD - Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310018, China. FAU - Fan, Xiaoxiao AU - Fan X AD - Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310018, China. FAU - Lin, Hui AU - Lin H AD - Department of General Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310018, China. AD - College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou 310027, China. AD - Zhejiang Key Laboratory of Intelligent Medical Decision Support, Taizhou Institute of Zhejiang University, Taizhou 318000, China. LA - eng GR - U23A20487、82573936、82300723、82670848/National Natural Science Foundation of China/ PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709253 OTO - NOTNLM OT - fluorescent polymers OT - local microviscosity OT - molecular tension OT - polymer formulation OT - solid stress OT - therapy monitoring OT - tumour mechanobiology COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:43 PHST- 2026/09/28 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - polym18202578 [pii] AID - polymers-18-02578 [pii] AID - 10.3390/polym18202578 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 19;18(20):2578. doi: 10.3390/polym18202578. PMID- 42942685 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 17 TI - Phenyl-Containing Silicone-Acrylate Core-Shell Particles for Transparent Toughening of PMMA with Enhanced Property Retention Under UVA Aging. LID - 10.3390/polym18202564 [doi] LID - 2564 AB - Transparent toughening of poly(methyl methacrylate) (PMMA) requires simultaneous control of mechanical energy dissipation and optical scattering, together with retention of properties under ultraviolet exposure. Here, silicone-acrylate (Si-ACR) core-shell particles comprising a PHSO-derived silicone-rich core and a PMMA-rich outer region were prepared by two-stage seeded emulsion polymerization without a deliberately added grafting agent. The phenyl-containing silicone-rich phase was selected to reduce the anticipated refractive-index contrast with PMMA. Si-ACR particles with an average diameter of 275 nm provided a favorable mechanical-optical balance. At 15 wt% Si-ACR, the notched Izod impact strength increased from 1.8 to 18.1 kJ/m(2), while total luminous transmittance remained 91.4% and 90.8% for 1 and 2 mm specimens, respectively, with haze below 0.8%. Fracture morphology was consistent with local debonding and/or void formation followed by enhanced plastic deformation of the PMMA matrix. After 500 h of accelerated UVA exposure, the blend retained an impact strength of 16.8 kJ/m(2) and a transmittance of 89.7% for the 1 mm specimen. These findings suggest potential applications in transparent protective shields and optical covers requiring improved impact resistance, low haze, and property retention under UVA exposure. FAU - Li, Yuao AU - Li Y AUID- ORCID: 0009-0002-8335-4519 AD - School of Polymer Science and Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. FAU - Yang, Fan AU - Yang F AUID- ORCID: 0009-0005-2609-2681 AD - School of Materials Science and Engineering, Ocean University of China, Qingdao 266404, China. FAU - Liu, Xiang AU - Liu X AUID- ORCID: 0009-0005-0175-2073 AD - School of Polymer Science and Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. FAU - Lin, Runxiong AU - Lin R AUID- ORCID: 0009-0006-7086-2986 AD - School of Polymer Science and Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. FAU - Zhuang, Tao AU - Zhuang T AUID- ORCID: 0009-0001-4216-4551 AD - School of Polymer Science and Engineering, Qingdao University of Science and Technology, Qingdao 266042, China. LA - eng PT - Journal Article DEP - 20261017 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709289 OTO - NOTNLM OT - UVA aging performance OT - poly(methyl methacrylate) OT - refractive-index contrast OT - silicone-acrylate core-shell particles OT - transparent toughening COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:43 PHST- 2026/09/21 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - polym18202564 [pii] AID - polymers-18-02564 [pii] AID - 10.3390/polym18202564 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 17;18(20):2564. doi: 10.3390/polym18202564. PMID- 42942681 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 17 TI - Rational Design of Molecularly Imprinted Nanozymes for Selective Degradation of Poly(bisphenol A carbonate) (BPA-PC). LID - 10.3390/polym18202560 [doi] LID - 2560 AB - Poly(bisphenol A carbonate) (BPA-PC) is an engineering thermoplastic whose recycling is hindered by its Code-7 classification and environmental BPA leaching. Here, we report water-soluble molecularly imprinted nanozymes for the selective depolymerization of BPA-PC under mild alkaline conditions. Synthesized within cross-linked surfactant micelles, the optimized nanozyme combines shape-complementary BPA-matched pockets with surface basicity, achieving a 72-fold rate enhancement over porcine pancreatic lipase. Fluorescence titrations with a soluble dansyl-labeled BPA-PC dimer confirm strong substrate recognition (K(a) ~10(6) M(-1)). Gel permeation chromatography reveals uniform reductions of 52-55% across M(n), M(w), and M(z), indicative of a random endo-chain cleavage mechanism. On macroscopic BPA-PC films, the nanozyme induces pronounced cracking and surface erosion, whereas polyethylene terephthalate (PET) control films remain undamaged. Furthermore, the aqueous nanozyme retains ~75% of its initial catalytic activity across five consecutive degradation cycles following simple phase extraction. This study demonstrates that tailored, shape-complementary nanozymes provide a generalizable, selective biocatalytic alternative for the circular chemical recycling of recalcitrant engineering plastics. FAU - Bui, Tien Tan AU - Bui TT AD - Department of Chemistry, Iowa State University, Ames, IA 50011-3111, USA. FAU - Zhao, Yan AU - Zhao Y AUID- ORCID: 0000-0003-1215-2565 AD - Department of Chemistry, Iowa State University, Ames, IA 50011-3111, USA. LA - eng GR - CHE-2246635 and CHE-2554710/U.S. National Science Foundation/ PT - Journal Article DEP - 20261017 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709287 OTO - NOTNLM OT - enzyme-mimetic catalysis OT - molecularly imprinted polymers OT - plastic recycling OT - poly(bisphenol A carbonate) (BPA-PC) degradation OT - polymer hydrolysis OT - substrate selectivity COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:43 PHST- 2026/08/05 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - polym18202560 [pii] AID - polymers-18-02560 [pii] AID - 10.3390/polym18202560 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 17;18(20):2560. doi: 10.3390/polym18202560. PMID- 42942671 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 16 TI - Response-Surface-Guided Extraction and Copper Modification of Nanocellulose from Corn Cob and Hemp Hurds: Source- and Loading-Dependent Structural and Electrochemical Properties. LID - 10.3390/polym18202547 [doi] LID - 2547 AB - Agricultural residues offer a source of nanocellulose for developing functional composites. This study examined how biomass source, extraction conditions, and copper loading affect the structural and electrochemical properties of nanocellulose derived from hemp hurds and corn cob. A citric-acid-assisted chemical-mechanical extraction route was investigated using response surface methodology, with extraction cycle number and sonication time as factors and FTIR-derived total crystallinity index and SEM-derived apparent fiber size as responses. The selected conditions were three cycles and 120 min for hemp hurds and three cycles and 75 min for corn cob, yielding measured mean apparent fiber sizes of 83.3 and 100 nm, respectively. Nanocellulose was modified with nominal Cu loadings of 15, 25, 50, and 75 wt%. At 0.1 Hz, impedance magnitude decreased from 274.15 and 247.67 kOmega for the respective bare references to below 10 kOmega for all Cu-modified groups. Cu incorporation also increased cycle-5 passed charge, with the corn-cob series showing its highest mean response at 50 wt%. FTIR, SEM, TGA, and XRD supported cellulose enrichment, nanoscale fibrillation, source-dependent thermal behavior, and crystalline metallic Cu formation. These findings support further evaluation of Cu-modified nanocellulose for electrochemical applications. FAU - Ranjbar-Navazi, Zahra AU - Ranjbar-Navazi Z AUID- ORCID: 0000-0002-0353-8696 AD - Department of Materials Science and Nanotechnology Engineering, Faculty of Engineering, Yeditepe University, 34755 Istanbul, Turkiye. LA - eng GR - project number 224M392/The Scientific and Technological Research Council of Turkiye/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13708852 OTO - NOTNLM OT - biomass OT - copper modification OT - corn cob OT - electrochemical properties OT - hemp hurds OT - nanocellulose OT - response surface methodology COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:43 PHST- 2026/09/30 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - polym18202547 [pii] AID - polymers-18-02547 [pii] AID - 10.3390/polym18202547 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 16;18(20):2547. doi: 10.3390/polym18202547. PMID- 42942652 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 15 TI - Carveol-Derived Cycloaliphatic Epoxy for Bio-Based High-Performance Thermosets. LID - 10.3390/polym18202531 [doi] LID - 2531 AB - Carveol, a terpenoid alcohol, was selected as a platform molecule for the development of high-performance bio-based epoxy thermosets. A cycloaliphatic diepoxy monomer, carveol dioxide (CDO), was synthesized and subsequently cured with branched polyethyleneimine (BPEI) to afford epoxy thermosets exhibiting outstanding thermomechanical properties, including a storage modulus of 2.5 GPa at 30 degrees C and a glass transition temperature (T(g)) of 97 degrees C. The resulting thermoset also exhibited strong adhesion to stainless steel, reaching a lap-shear strength of 10.1 MPa, which compares favorably with previously reported bio-based epoxy adhesives. To further enhance network performance, the pendant secondary hydroxyl group of CDO was exploited to introduce urethane crosslinks prior to epoxy curing. Urethane incorporation markedly increased the crosslink density and raised T(g) to 137 degrees C, which, to the best of our knowledge, is the highest value reported for terpene-based epoxy thermosets. These results highlight carveol as a promising renewable platform for high-performance epoxy thermosets and adhesives because its unique combination of C=C bonds and a secondary hydroxyl group enables both epoxide formation and subsequent network modification. FAU - Lim, Chae Hyun AU - Lim CH AUID- ORCID: 0009-0003-0189-9345 AD - Department of Chemical Engineering and Materials Science, College of Biochemical Engineering, College of Convergence Engineering, Sangmyung University, Seoul 03016, Republic of Korea. FAU - Kim, Miji AU - Kim M AD - Department of Chemical Engineering and Materials Science, College of Biochemical Engineering, College of Convergence Engineering, Sangmyung University, Seoul 03016, Republic of Korea. FAU - Yun, Seok Il AU - Yun SI AD - Department of Chemical Engineering and Materials Science, College of Biochemical Engineering, College of Convergence Engineering, Sangmyung University, Seoul 03016, Republic of Korea. LA - eng GR - 2025-A000-0183/Sangmyung University/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13708782 OTO - NOTNLM OT - bio-derived epoxy OT - carveol OT - terpene OT - urethane crosslinking COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:43 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - polym18202531 [pii] AID - polymers-18-02531 [pii] AID - 10.3390/polym18202531 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 15;18(20):2531. doi: 10.3390/polym18202531. PMID- 42942643 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 15 TI - Lignin-Derived Diphenylamines for Thermo-Oxidative Stabilization of Natural Rubber. LID - 10.3390/polym18202526 [doi] LID - 2526 AB - Sustainable polymer stabilization requires renewable antioxidants that combine efficient radical scavenging with clear structure-performance relationships. Diphenylamine antioxidants are widely used in rubber protection, but their conventional production depends on petrochemical feedstocks and hazardous aniline chemistry. Lignin valorization research largely targets model compounds; native lignin's complexity makes direct conversion challenging. Here, we report a two-step tandem strategy that converts raw lignin into diphenylamine compounds without intermediate purification. PtFe/NiAl(2)O(4) and phosphotungstic acid first depolymerize lignin selectively to p-substituted phenols, which are then converted into diphenylamine derivatives with ammonium formate, Pd/C and LiOH. The process affords p-substituted phenols in 35 wt% yield and final diphenylamine compounds in 23 wt% yield from birch lignin. When incorporated into natural rubber at 1 wt%, the lignin-derived diphenylamines suppress thermo-oxidative aging: after treatment at 140 degrees C for 24 h, the mass loss decreases from 10% for the untreated rubber to 1% for the stabilized material. The stabilized rubber also withstands harsher aging conditions and shows performance comparable to commercial EPDM rubber. Mechanistic experiments indicate that lignin demethoxylation supplies in situ hydrogen for depolymerization, while the diphenylamine N-H group interrupts radical chain oxidation in the rubber matrix. This work links lignin valorization chemistry with polymer-aging control and provides a renewable route to high-performance rubber antioxidants. FAU - Wang, Zhongwei AU - Wang Z AD - Guizhou Academy of Forestry, Guiyang 550005, China. AD - College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410000, China. FAU - Yu, Zhaozhuo AU - Yu Z AD - Guizhou Academy of Forestry, Guiyang 550005, China. FAU - Liu, Zhu AU - Liu Z AD - Guizhou Academy of Forestry, Guiyang 550005, China. AD - College of Materials Science and Engineering, Central South University of Forestry and Technology, Changsha 410000, China. FAU - Zeng, Qinghan AU - Zeng Q AD - Guizhou Academy of Forestry, Guiyang 550005, China. FAU - Luo, Mei AU - Luo M AD - Guizhou Academy of Forestry, Guiyang 550005, China. FAU - Tang, Yipeng AU - Tang Y AD - Guizhou Academy of Forestry, Guiyang 550005, China. FAU - Ji, Ning AU - Ji N AD - Guizhou Academy of Forestry, Guiyang 550005, China. FAU - Yang, Wulin AU - Yang W AD - Guizhou Academy of Forestry, Guiyang 550005, China. FAU - Yang, Shoulu AU - Yang S AD - Guizhou Academy of Forestry, Guiyang 550005, China. LA - eng GR - 32260361/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709165 OTO - NOTNLM OT - diphenylamine OT - lignin OT - natural rubber OT - thermo-oxidative aging COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:43 PHST- 2026/07/11 00:00 [received] PHST- 2026/08/31 00:00 [revised] PHST- 2026/08/31 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - polym18202526 [pii] AID - polymers-18-02526 [pii] AID - 10.3390/polym18202526 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 15;18(20):2526. doi: 10.3390/polym18202526. PMID- 42942637 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 14 TI - Insights on Novel Dental Resin Composites: A Scoping Review. LID - 10.3390/polym18202516 [doi] LID - 2516 AB - Dental resin composites (DRCs) are widely used restorative materials because of their aesthetics, ease of handling, and compatibility with minimally invasive approaches. Research continues to improve their formulations and properties. This scoping review aimed to summarize and analyze evidence on novel DRCs and their potential development, and to discuss current trends, technologies, and research areas that may shape their evolution. A systematic search of PubMed and Scopus was conducted for studies published or available online from 2010 to 2024. Fifty-nine studies were included in the qualitative synthesis. The included studies described innovations designed to address clinical needs through bioactive materials, antibacterial agents, sustainable fillers, modified monomer systems, and other functional strategies. Future research should optimize additive concentrations, evaluate long-term clinical performance, and address cost-effectiveness, aesthetic limitations, standardization, and translational feasibility. FAU - Ping, Lau Kian AU - Ping LK AD - School of Dental Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kota Bharu 16150, Kelantan, Malaysia. FAU - Al-Rawas, Matheel AU - Al-Rawas M AUID- ORCID: 0000-0001-6919-5334 AD - Prosthodontic Unit, School of Dental Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kota Bharu 16150, Kelantan, Malaysia. FAU - Abuhijleh, Eyas AU - Abuhijleh E AUID- ORCID: 0000-0003-0423-2469 AD - Department of Clinical Sciences, Center of Medical and Bio-Allied Health Sciences Research, College of Dentistry, Ajman University, Ajman 346, United Arab Emirates. FAU - Mohammed, Sohaib Fadhil AU - Mohammed SF AUID- ORCID: 0000-0001-9282-467X AD - Department of Operative Dentistry, College of Dentistry, University of Anbar, Baghdad 55431, Iraq. FAU - Reza, Nabeel AU - Reza N AUID- ORCID: 0009-0007-0393-9573 AD - School of Dental Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kota Bharu 16150, Kelantan, Malaysia. FAU - Johari, Yanti AU - Johari Y AD - School of Dentistry, Management and Science University, University Drive Off Persiaran Olahraga, Section 13, Shah Alam 40100, Selangor, Malaysia. FAU - Mohamad, Dasmawati AU - Mohamad D AD - Biomaterial Unit, School of Dental Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kota Bharu 16150, Kelantan, Malaysia. FAU - Abdullah, Johari Yap AU - Abdullah JY AUID- ORCID: 0000-0002-6147-4192 AD - Craniofacial Imaging Laboratory, School of Dental Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kota Bharu 16150, Kelantan, Malaysia. FAU - Noorani, Tahir Yusuf AU - Noorani TY AUID- ORCID: 0000-0002-4661-7458 AD - Conservative Dentistry Unit, School of Dental Sciences, Universiti Sains Malaysia, Health Campus, Kubang Kerian, Kota Bharu 16150, Kelantan, Malaysia. LA - eng GR - FRGS/1/2024/SKK11/USM/03/1/Ministry of Higher Education/ PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709035 OTO - NOTNLM OT - dental resin composites OT - experimental composite resins OT - novel dental composites OT - novel dental materials OT - restorative dentistry COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:43 PHST- 2026/09/12 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - polym18202516 [pii] AID - polymers-18-02516 [pii] AID - 10.3390/polym18202516 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 14;18(20):2516. doi: 10.3390/polym18202516. PMID- 42942636 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 14 TI - Conducting Polymers in Electrolyte and Separator Engineering for Zn-Ion Hybrid Capacitors. LID - 10.3390/polym18202514 [doi] LID - 2514 AB - Conducting polymers (CPs) have emerged as multifunctional components that modulate electrolyte and separator design for Zn-ion hybrid capacitors, with selected Zn-based battery and alkali-ion systems used as mechanistic references rather than direct performance evidence. Their pi-conjugated backbones, tunable doping states, and reversible redox chemistry enable coupled ion transport, interfacial charge regulation, and mechanically compliant ion-conducting environments. However, CP integration also requires careful control of electronic leakage, self-discharge, short-circuit risk, and redox-state-dependent swelling, particularly when CP domains are introduced into separator architectures. This review examines how CP-containing electrolytes and separator-related architectures can regulate ion transport and interfacial charge distribution, provided that continuous electronic pathways are suppressed. In aqueous, quasi-solid gel, solid polymer, and redox-active electrolytes, CP-containing domains are examined in terms of ionic conductivity, Zn(2+) transport, solvation regulation, and polarization mitigation. In separator-related architectures, spatially confined and electronically interrupted CP domains are discussed with emphasis on ion-flux homogenization, dendrite suppression, electron-blocking requirements, and dimensional stability. Comparative analyses separate directly demonstrated Zn-ion hybrid capacitor behavior from mechanisms inferred from related ion-storage systems. Finally, future directions are discussed for quantitative benchmarking, scalable CP processing, and architecture-controlled CP domains for coordinated electrolyte and separator design. FAU - Nguyen, Thi Thuong Thuong AU - Nguyen TTT AUID- ORCID: 0009-0009-4767-1833 AD - Department of Polymer Engineering, Graduate School, Chonnam National University, 77 Yongbong-ro, Gwangju 61186, Republic of Korea. FAU - Su, Yichun AU - Su Y AD - School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China. FAU - Le, Cuong Van AU - Le CV AUID- ORCID: 0000-0002-9196-351X AD - Department of Polymer Engineering, Graduate School, Chonnam National University, 77 Yongbong-ro, Gwangju 61186, Republic of Korea. FAU - Yoon, Hyeonseung AU - Yoon H AUID- ORCID: 0009-0001-0892-6436 AD - School of Polymer Science and Engineering, Chonnam National University, 77 Yongbong-ro, Gwangju 61186, Republic of Korea. FAU - Liu, Ping AU - Liu P AUID- ORCID: 0000-0002-5003-6314 AD - School of Petrochemical Engineering, Changzhou University, Changzhou 213164, China. AD - Jiangsu Province Engineering Research Center of Biodegradable Materials, Changzhou University, Changzhou 213164, China. FAU - Yoon, Hyeonseok AU - Yoon H AUID- ORCID: 0000-0002-5403-1617 AD - Department of Polymer Engineering, Graduate School, Chonnam National University, 77 Yongbong-ro, Gwangju 61186, Republic of Korea. AD - School of Polymer Science and Engineering, Chonnam National University, 77 Yongbong-ro, Gwangju 61186, Republic of Korea. LA - eng GR - 2025-0091-01/Chonnam National University/ GR - CQ20254007/Education Department of Jiangsu Province/ GR - Qing Lan Project/Education Department of Jiangsu Province/ PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709057 OTO - NOTNLM OT - conducting polymer OT - electrolyte OT - energy storage OT - hybrid capacitor OT - separator COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:43 PHST- 2026/09/05 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - polym18202514 [pii] AID - polymers-18-02514 [pii] AID - 10.3390/polym18202514 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 14;18(20):2514. doi: 10.3390/polym18202514. PMID- 42942634 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 14 TI - Mechanical and Thermomechanical Performance Evaluation of CFRP Sandwich Structures with Additively Manufactured Composite Cores. LID - 10.3390/polym18202510 [doi] LID - 2510 AB - This study experimentally evaluates how various 3D-printed gyroid core materials influence the mechanical and thermo-mechanical performance of lightweight carbon-fiber-reinforced polymer (CFRP) sandwich structures. Three gyroid core configurations were considered: short-carbon-fiber-reinforced polyphthalamide (PPA-CF), continuous-carbon-fiber-reinforced nylon (ONYX-R), and nylon short-carbon-fiber-reinforced (ONYX), all combined with similar 0/90 degrees carbon-fiber-reinforced polymer composite (CFRP) face sheets. The sandwich specimens were manufactured using a one-step co-curing process and characterized by flatwise compression, three-point bending, and dynamic mechanical analysis (DMA). The PPA-CF-CFRP sandwich configuration exhibited the highest flexural performance, with a maximum flexural stress of 148-156 MPa, approximately 39% higher than that of ONYX-R-CFRP, and the highest mean compression modulus of approximately 87 MPa. However, ONYX-R-CFRP achieved the highest mean compressive strength of 12.84 MPa, compared with 11.85 MPa for PPA-CF-CFRP. These findings indicate that the mechanical response depends on the loading mode and is influenced by the combined effects of the thermoplastic matrix, carbon-fiber reinforcement architecture, and the resulting structural characteristics of the printed gyroid cores. DMA revealed that the PPA-CF-CFRP sandwich achieved a storage modulus of ~9 GPa at 30 degrees C, outperforming the ONYX-R-CFRP configuration by approximately 70%. Conversely, ONYX-R-CFRP exhibited the highest damping capacity, with a maximum tan delta of approximately 0.16, about 60% higher than the PPA-CF-CFRP configuration. The ONYX-CFRP sandwich configuration displayed the lowest mechanical and thermo-mechanical performance, demonstrating that the stiffness, deformation capacity, damping response, and temperature-dependent mechanical behavior of these sandwich structures can be tailored through the selection of the additively manufactured core configuration. FAU - Ciubotariu, Alexandru AU - Ciubotariu A AD - Composite Materials Laboratory for Aeronautical Field, Romanian Research & Development Institute for Gas Turbines-COMOTI, 220D Iuliu Maniu Av., 061126 Bucharest, Romania. FAU - Badea, Teodor-Adrian AU - Badea TA AUID- ORCID: 0000-0002-7772-1207 AD - Composite Materials Laboratory for Aeronautical Field, Romanian Research & Development Institute for Gas Turbines-COMOTI, 220D Iuliu Maniu Av., 061126 Bucharest, Romania. FAU - Mustareata, Madalina AU - Mustareata M AD - Composite Materials Laboratory for Aeronautical Field, Romanian Research & Development Institute for Gas Turbines-COMOTI, 220D Iuliu Maniu Av., 061126 Bucharest, Romania. FAU - Maier, Raluca AU - Maier R AUID- ORCID: 0000-0001-6792-4425 AD - Composite Materials Laboratory for Aeronautical Field, Romanian Research & Development Institute for Gas Turbines-COMOTI, 220D Iuliu Maniu Av., 061126 Bucharest, Romania. FAU - Timonia, Andrei AU - Timonia A AD - Composite Materials Laboratory for Aeronautical Field, Romanian Research & Development Institute for Gas Turbines-COMOTI, 220D Iuliu Maniu Av., 061126 Bucharest, Romania. FAU - Buga, Vlad AU - Buga V AD - Composite Materials Laboratory for Aeronautical Field, Romanian Research & Development Institute for Gas Turbines-COMOTI, 220D Iuliu Maniu Av., 061126 Bucharest, Romania. FAU - Petre, Laurentiu AU - Petre L AD - Composite Materials Laboratory for Aeronautical Field, Romanian Research & Development Institute for Gas Turbines-COMOTI, 220D Iuliu Maniu Av., 061126 Bucharest, Romania. FAU - Moraras, Ciprian Ionut AU - Moraras CI AUID- ORCID: 0000-0001-6300-2255 AD - Mechanical Engineering, Mechatronics and Robotics Department, Mechanical Engineering Faculty, "Gheorghe Asachi" Technical University of Iasi, 700050 Iasi, Romania. FAU - Goanta, Viorel AU - Goanta V AUID- ORCID: 0000-0002-1693-5076 AD - Mechanical Engineering, Mechatronics and Robotics Department, Mechanical Engineering Faculty, "Gheorghe Asachi" Technical University of Iasi, 700050 Iasi, Romania. LA - eng GR - 31N/2023/This research received funding from the Ministry of Research, Innovation and Digitization through the "Nucleu" Program, Grant no. 31N/2023/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709308 OTO - NOTNLM OT - 3D-printed core OT - CFRP composite OT - dynamic mechanical analysis (DMA) OT - flatwise compression OT - flexural tests OT - sandwich structure COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:43 PHST- 2026/09/30 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - polym18202510 [pii] AID - polymers-18-02510 [pii] AID - 10.3390/polym18202510 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 14;18(20):2510. doi: 10.3390/polym18202510. PMID- 42942633 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 14 TI - Influence of Furfuryl Alcohol on the Cross-Linking Process of Selected Industrial Phenol-Formaldehyde Resins. LID - 10.3390/polym18202513 [doi] LID - 2513 AB - Furfuryl alcohol (C(5)H(6)O(2), FA) is commonly used as a modifier for phenol-formaldehyde (PF) resins and has been reported to influence their curing behavior and processing performance. Comparative analysis of commercial resin systems with and without FA suggested that the presence of FA influences the distribution of methylene bridge linkages formed during curing. FT-IR spectra indicated an increased contribution of para-para linkages in FA-containing resins, with this tendency becoming more pronounced at elevated temperatures. MAS-NMR spectra revealed differences in local methylene bridge environments, supporting the conclusion that FA influences the organization of the cross-linked network. XPS analysis revealed differences in the chemical structure of the cured resins, including a higher contribution of oxygen-containing functional groups in the FA-modified systems. STA-FT-IR results showed that FA-containing resins exhibited a more gradual curing process accompanied by a more uniform release of volatile products. Dynamic viscosity measurements demonstrated a substantial reduction in viscosity after FA addition, from 1051 to 228 mPa s for the F110-based resin system and from 1104 to 144 mPa s for the MC-05-based system. The results suggest that FA influences the cross-linking pathway, curing behavior and processing properties of PF resins. Improved understanding of these effects may support advanced PF-based composites manufacture. FAU - Gubernat, Maciej AU - Gubernat M AUID- ORCID: 0000-0002-5424-1091 AD - Faculty of Materials Science and Ceramics, AGH University of Krakow, Av. Mickiewicza 30, 30-059 Krakow, Poland. FAU - Jelen, Piotr AU - Jelen P AUID- ORCID: 0000-0003-2905-6078 AD - Faculty of Materials Science and Ceramics, AGH University of Krakow, Av. Mickiewicza 30, 30-059 Krakow, Poland. FAU - Marchewka, Jakub AU - Marchewka J AUID- ORCID: 0000-0002-3475-5984 AD - Faculty of Materials Science and Ceramics, AGH University of Krakow, Av. Mickiewicza 30, 30-059 Krakow, Poland. FAU - Benko, Aleksandra AU - Benko A AUID- ORCID: 0000-0001-7474-3374 AD - Faculty of Materials Science and Ceramics, AGH University of Krakow, Av. Mickiewicza 30, 30-059 Krakow, Poland. FAU - Wielowski, Ryszard AU - Wielowski R AUID- ORCID: 0009-0005-6565-1984 AD - Faculty of Materials Science and Ceramics, AGH University of Krakow, Av. Mickiewicza 30, 30-059 Krakow, Poland. FAU - Zambrzycki, Marcel AU - Zambrzycki M AUID- ORCID: 0000-0002-7941-426X AD - Faculty of Materials Science and Ceramics, AGH University of Krakow, Av. Mickiewicza 30, 30-059 Krakow, Poland. FAU - Sokolowski, Krystian AU - Sokolowski K AUID- ORCID: 0000-0001-8576-2191 AD - Academic Centre for Materials and Nanotechnology, AGH University of Krakow, Av. Mickiewicza 30, 30-059 Krakow, Poland. FAU - Fraczek-Szczypta, Aneta AU - Fraczek-Szczypta A AUID- ORCID: 0000-0002-2055-1489 AD - Faculty of Materials Science and Ceramics, AGH University of Krakow, Av. Mickiewicza 30, 30-059 Krakow, Poland. LA - eng GR - LIDER13/0108/2022/National Centre for Research and Development/ GR - Grant No. 13501/Excellence Initiative - Research University program at AGH University of Krakow/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13708871 OTO - NOTNLM OT - carbon-carbon composite precursor OT - curing behavior OT - furfuryl alcohol OT - methylene bridges OT - phenol-formaldehyde resin COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:43 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - polym18202513 [pii] AID - polymers-18-02513 [pii] AID - 10.3390/polym18202513 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 14;18(20):2513. doi: 10.3390/polym18202513. PMID- 42942629 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 14 TI - Development and Optimization of a Molecularly Imprinted Polymer for Electrochemical Detection of Escherichia coli. LID - 10.3390/polym18202508 [doi] LID - 2508 AB - In this study, a molecularly imprinted polymer (MIP)-based electrochemical biosensor was developed for the selective detection of Escherichia coli (E. coli). The MIP recognition layer was synthesized by free-radical polymerization using MAM, AAM and VP as functional monomers with E. coli cells as the imprinting template. The MIP was immobilized onto a screen-printed electrode and evaluated using cyclic voltammetry (CV). Six MIP formulations with different MAM:AAM ratios were investigated to determine the optimal monomer composition. The optimized formulation, with an MAM:AAM ratio of 2:3:1, was evaluated over an E. coli concentration range of 10-10(5) CFU/mL and exhibited a linear response with a slope of 4.1107 and an R(2) value of 0.9893 with a detection limit of 1.52 CFU/mL. The selectivity of the optimized sensor was further evaluated using Enterococcus faecalis and Pseudomonas aeruginosa, which showed lower responses than E. coli. These results indicate that the proposed MIP-based electrochemical biosensor provides preferential recognition of E. coli under the investigated conditions and demonstrates potential for bacterial detection. FAU - Pintavirooj, Chuchart AU - Pintavirooj C AD - Department of Biomedical Engineering, School of Engineering, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand. FAU - Vongmanee, Naphatsawan AU - Vongmanee N AD - Department of Physics, Faculty of Science, King Mongkut's University of Technology Thonburi, Bangkok 10140, Thailand. FAU - Visitsattapongse, Sarinporn AU - Visitsattapongse S AUID- ORCID: 0009-0004-5890-6427 AD - Department of Biomedical Engineering, School of Engineering, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand. LA - eng GR - grant number RE-KRIS/FF68/088./KMITL which has been received funding support from the NSRF/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13708840 OTO - NOTNLM OT - Escherichia coli OT - electrochemical sensor OT - molecularly imprinted polymer OT - screen-printed electrode COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:43 PHST- 2026/09/05 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - polym18202508 [pii] AID - polymers-18-02508 [pii] AID - 10.3390/polym18202508 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 14;18(20):2508. doi: 10.3390/polym18202508. PMID- 42942628 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 13 TI - Multiscale Physicochemical Response of Lignocellulosic Carludovica palmata Fibers to Controlled Drying and Alkaline Hydrogen Peroxide Treatment. LID - 10.3390/polym18202507 [doi] LID - 2507 AB - Processing can alter the optical, structural, spectroscopic, morphological, and thermal characteristics of lignocellulosic fibers in different ways. Five measured states of Carludovica palmata were compared: an artisan-processed reference (PJ-0), water-conditioned fibers dried for 1 h at 84 and 104 degrees C (SPJ-84 and SPJ-104), and SPJ-104 fibers treated with two nominal alkaline hydrogen peroxide formulations (BPJ-3% and BPJ-6%). Relative image-based color analysis, X-ray diffraction (XRD), ATR-FTIR spectroscopy, scanning electron microscopy (SEM) coupled with fractal, multifractal, orientation, and spatial-texture analyses, and TGA/DTG were integrated within the same processing series. The BPJ-3% and BPJ-6% images showed similar measured appearance in the relative image-based color analysis, with a pairwise DeltaE00=1.7, whereas their selected SEM fields differed in directional and boundary organization. The selected BPJ-3% field showed higher axial anisotropy (A=0.2835) than BPJ-6% (A=0.1183), while the selected BPJ-6% field showed DB=1.5570 and a singularity-spectrum width of Deltaalpha=1.3444. In the individual XRD profiles, BPJ-3% showed higher apparent Scherrer and Segal descriptors than BPJ-6%, whereas the individual BPJ-6% TGA/DTG profile showed the highest principal degradation-rate temperature of the series (Tmax=322.6 degrees C). Because the principal physicochemical series contained one independent specimen per condition and one SEM, XRD, and TGA/DTG measurement set per condition, these numerical differences are interpreted as specimen-level observations rather than reproducible treatment-level effects. The study therefore provides an integrated multiscale description of the measured fiber states while explicitly delimiting the level of inference supported by the experimental design. FAU - Ayala-Chauvin, Manuel AU - Ayala-Chauvin M AUID- ORCID: 0000-0002-3911-377X AD - Centro de Investigacion en Ciencias Humanas y de la Educacion (CICHE), Facultad de Ciencias de la Educacion, Universidad Tecnologica Indoamerica, Ambato 180103, Ecuador. FAU - Ojeda, Sebastian AU - Ojeda S AUID- ORCID: 0009-0002-8377-701X AD - Centro de Investigacion en Ciencias Humanas y de la Educacion (CICHE), Facultad de Ciencias de la Educacion, Universidad Tecnologica Indoamerica, Ambato 180103, Ecuador. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709129 OTO - NOTNLM OT - Carludovica palmata OT - cellulose OT - colorimetry OT - cultural heritage OT - fractal analysis OT - lignin OT - lignocellulosic fibers OT - natural polymers OT - thermal stability COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:43 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - polym18202507 [pii] AID - polymers-18-02507 [pii] AID - 10.3390/polym18202507 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 13;18(20):2507. doi: 10.3390/polym18202507. PMID- 42942624 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 13 TI - Material-Efficient Selection of Externally Bonded Fiber-Reinforced Polymer Composites for RC Shear Strengthening Using a Literature-Based Database and Break-Even Criteria. LID - 10.3390/polym18202503 [doi] LID - 2503 AB - Fiber-reinforced polymer (FRP) shear strengthening is generally selected on the basis of structural capacity, whereas the efficiency with which installed material contributes to shear resistance has received little quantitative attention. This study develops a literature-based material-efficiency framework from a database of 359 published tests compiled from 43 experimental programs covering carbon, glass, aramid, basalt, and PET-FRP systems. Material efficiency (ME) was defined as the experimentally attributed FRP shear contribution per unit mass of installed composite sheet. ME varied approximately 30-fold. Median ME was 303 kN.m.kg(-1) for CFRP and 38 kN.m.kg(-1) for GFRP, giving a CFRP:GFRP median-efficiency ratio of 8.1 (program-clustered 95% CI: 3.8-18.3; 2.8-21.3 when thickness-convention and density uncertainty were also propagated). This observational, database-level ratio, which is an association rather than a causal material effect, was translated into a sheet-level cradle-to-gate greenhouse-gas break-even screening criterion. A response-surface model based on XGBoost achieved random ten-fold out-of-fold R(2) = 0.802, decreasing to 0.526 under program-grouped validation and 0.394 under leave-one-program-out validation; most of this loss reflected program-level offsets. Member geometry and transverse reinforcement carried greater predictive importance for the attributed FRP contribution than FRP-sheet properties under both random and program-grouped validation. Material efficiency generally decreased with increasing installed sheet rigidity, consistent with diminishing structural returns from additional FRP material. The framework compares code-compliant alternatives that satisfy the required FRP shear contribution, using material efficiency to identify lower-material-demand solutions before project-specific environmental and economic evaluation. FAU - Ali, Nageh M AU - Ali NM AUID- ORCID: 0000-0002-4178-2681 AD - Department of Civil and Environmental Engineering, College of Engineering, Majmaah University, Al-Majmaah 11952, Saudi Arabia. FAU - Farghaly, Ahmed AU - Farghaly A AD - Department of Civil and Environmental Engineering, College of Engineering, Majmaah University, Al-Majmaah 11952, Saudi Arabia. LA - eng GR - R-2026-398/Majmaah University/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13708974 OTO - NOTNLM OT - FRP shear strengthening OT - externally bonded FRP OT - greenhouse-gas break-even OT - material efficiency OT - polymer composites OT - reinforced concrete OT - sustainable strengthening COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:43 PHST- 2026/09/13 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - polym18202503 [pii] AID - polymers-18-02503 [pii] AID - 10.3390/polym18202503 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 13;18(20):2503. doi: 10.3390/polym18202503. PMID- 42942612 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 12 TI - Effect of Thermal Processing Parameters on the Tensile Properties of FDM-Printed ABS Components. LID - 10.3390/polym18202491 [doi] LID - 2491 AB - Fused Deposition Modeling (FDM) offers manufacturing flexibility for acrylonitrile butadiene styrene (ABS), but mechanical performance is sensitive to thermal history during printing. This study examines the effects of printing temperature (PT), chamber temperature (CT), and cooling fan speed (CFS) on the tensile properties of FDM-printed ABS. A face-centered central composite design (75 specimens, 15 conditions, five replicates) was used to fit second-order models for ultimate tensile strength (UTS), tensile toughness (T, calculated as the area under the stress-strain curve up to fracture), and break strain (epsilonB). PT was the dominant factor for all three responses (15-29% of total variance), with lower PT yielding higher strength, toughness, and ductility. A significant chamber-temperature x cooling-fan-speed interaction was identified for UTS (F=9.13, p=0.004), requiring joint optimization for strength. CT had no statistically significant effect on toughness or break strain, either alone or in interaction, within the tested range. Single-response optimization identified PT = 220 degrees C, CT = 42 degrees C, CFS = 50% as maximizing UTS (29.6 MPa); the point estimates for toughness and break strain were nominally higher at CT = 58 degrees C, but a formal contrast between CT = 42 and 58 degrees C showed 95% confidence intervals that included zero for both responses, so this difference cannot be resolved from noise. PT = 220 degrees C and CFS = 50% are robust recommendations across all models and weightings tested, while the optimal CT for toughness and break strain remains unresolved within this design. These findings provide a statistically grounded framework for selecting thermal process parameters for FDM-printed ABS components. FAU - Mukras, Saad M S AU - Mukras SMS AUID- ORCID: 0000-0001-5662-518X AD - Department of Mechanical Engineering, College of Engineering, Qassim University, Buraidah 51452, Saudi Arabia. FAU - Omar, Hanafy M AU - Omar HM AUID- ORCID: 0000-0002-6796-1186 AD - Department of Mechanical Engineering, College of Engineering, Qassim University, Buraidah 51452, Saudi Arabia. FAU - Alzughaibi, Saleh AU - Alzughaibi S AUID- ORCID: 0009-0003-6683-0277 AD - Department of Mechanical Engineering, College of Engineering, Qassim University, Buraidah 51452, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261012 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709048 OTO - NOTNLM OT - ABS OT - fused deposition modeling OT - process optimization OT - response surface methodology OT - tensile properties OT - thermal processing parameters COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:43 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - polym18202491 [pii] AID - polymers-18-02491 [pii] AID - 10.3390/polym18202491 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 12;18(20):2491. doi: 10.3390/polym18202491. PMID- 42942610 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 12 TI - Review of Sintering Methods and Polymer Materials for Powder Bed Fusion Technology. LID - 10.3390/polym18202489 [doi] LID - 2489 AB - As a sustainable additive manufacturing method, powder bed fusion (PBF) technology has emerged as one of the important fabrication methods for mass production. The technology has several advantages, such as environmental friendliness, recyclability of materials, and being support-free, which make it have great potential in high-efficiency and green manufacturing. The sintering methods for PBF technology mainly have three technical routes: (1) conventional selective laser sintering (SLS); (2) high-speed sintering (HSS)/multi-jet fusion (MJF); (3) microwave heating (MH). This work systematically analyzes the sintering methods with their sintering characteristics and the corresponding polymer materials applied in PBF technology. The SLS method is the most widely used sintering method in PBF technology, with virtues such as cost-effectiveness, feasibility, being a robust technique and requiring no support. HSS and MJF are more efficient based on ink-jet technology. MH can assist other sintering methods with penetrating polymer powders to achieve uniform bulk heating. Commonly used polymer materials include polyamide, polypropylene, polyether ether ketone, thermoplastic polyurethane, etc. Generally, the selection of polymers is decided by the application demands. Most polymers can be recycled or reused in PBF technology. Finally, the development trends of sintering methods for PBF technology, including high-efficiency, low-energy-consumption sintering, multi-material composite sintering, and the application of new polymer materials, are prospected. This work aims to offer technical guidance for the optimization of sintering processes and the development of new polymer powder materials, thereby promoting the advancement of PBF technology. FAU - Zhou, Haihua AU - Zhou H AUID- ORCID: 0000-0002-7270-8388 AD - Key Laboratory of Green Printing, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. AD - CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. FAU - Yang, Ming AU - Yang M AUID- ORCID: 0009-0006-8063-1145 AD - Key Laboratory of Green Printing, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. AD - CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. FAU - Song, Yanlin AU - Song Y AD - Key Laboratory of Green Printing, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. AD - CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. LA - eng GR - 2023YFB4603500/National Key R&D Program of China/ PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709225 OTO - NOTNLM OT - additive manufacturing OT - high-speed sintering OT - microwave heating OT - multi-jet fusion OT - powder bed fusion OT - selective laser sintering COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:43 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - polym18202489 [pii] AID - polymers-18-02489 [pii] AID - 10.3390/polym18202489 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 12;18(20):2489. doi: 10.3390/polym18202489. PMID- 42942605 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 11 TI - Interleukin Expression and Activation of Inflammatory/Apoptosis Pathways on Human Gingival Fibroblasts Exposed to Light-Cured Dental Composites. LID - 10.3390/polym18202484 [doi] LID - 2484 AB - This in vitro study aimed to analyze the activation of the NFkappaB p65/MyD88/NALP3 inflammatory pathway and the expression of the BCL-2/BAX/Caspase-3 apoptosis pathway on human gingival fibroblasts (hGFs) after 24 h and 1-week exposure to the eluates of three light-cured commercial composites (GR: GrandioSO, VOCO; BF: Enamel Plus HRi Biofunction, Micerium; FU: Filtek universal restorative, 3M) and the production of pro-inflammatory cytokines (TNF-alpha, IL-6, IL-1beta). A total of 50 light-cured disk-shaped specimens for each composite group (GR, BF and FU) were produced. The tested materials differed in resin matrix composition; notably, GR contained Bis-GMA, whereas BF and FU contained urethane-based dimethacrylates. The hGFs were exposed for 24 h and 1 week to eluates of each group. Pathway activation was evaluated by cell metabolic activity, immunofluorescence analysis and real-time PCR. Inflammatory effects were assessed in hGF culture by measuring proinflammatory cytokine production (ELISA). GR, BF and FU showed a reduction in metabolic activity in a time-dependent manner. GR showed a significant decrease in terms of cell viability after 1 week of exposure and a greater expression of proapoptotic markers than BF and FU. High levels of proinflammatory cytokines were measured after 1 week of culture for GR. The inflammatory pathway was activated in a time-dependent manner. Pathway activation was material-dependent; among the tested composites, GR was found to be more cytotoxic than BF and FU. Despite the limitations of an in vitro study, these findings suggest that dental composite eluates cause time- and material-dependent cytotoxicity, inflammation, and apoptosis in hGFs. Exposure significantly impairs cellular metabolic activity from 24 h up to 1 week. Among the evaluated materials, the GR composite triggered a markedly severe inflammatory and apoptotic effect compared to BF or FU. Ultimately, these findings underscore the potential clinical importance of selecting materials with optimal biocompatibility to minimize adverse tissue reactions, although their direct translation into clinical practice would require further research. FAU - Sorrentino, Edoardo AU - Sorrentino E AUID- ORCID: 0009-0001-5547-7266 AD - Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy. FAU - D'Arcangelo, Camillo AU - D'Arcangelo C AD - Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio" Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy. FAU - Mazzone, Antonella AU - Mazzone A AUID- ORCID: 0009-0003-7290-0219 AD - Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy. FAU - Fonticoli, Luigia AU - Fonticoli L AD - Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy. FAU - Frisone, Alessio AU - Frisone A AD - Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy. FAU - Coppola, Ludovica AU - Coppola L AUID- ORCID: 0009-0006-5290-9832 AD - Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio" Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy. FAU - Mazzon, Emanuela AU - Mazzon E AD - Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy. FAU - Trubiani, Oriana AU - Trubiani O AUID- ORCID: 0000-0002-7459-4898 AD - Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy. FAU - Pizzicannella, Jacopo AU - Pizzicannella J AUID- ORCID: 0000-0003-3318-3758 AD - Department of Neuroscience, Imaging and Clinical Sciences, University "G. d'Annunzio" Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy. FAU - Marconi, Guya Diletta AU - Marconi GD AUID- ORCID: 0000-0001-7754-4653 AD - Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy. FAU - Diomede, Francesca AU - Diomede F AUID- ORCID: 0000-0002-0384-6509 AD - Department of Innovative Technologies in Medicine and Dentistry, University "G. d'Annunzio" Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy. FAU - De Angelis, Francesco AU - De Angelis F AUID- ORCID: 0000-0001-7369-2667 AD - Department of Medical, Oral and Biotechnological Sciences, University "G. d'Annunzio" Chieti-Pescara, Via dei Vestini 31, 66100 Chieti, Italy. LA - eng GR - ECS00000041-VITALITY-CUP: D73C22000840006/Ministero dell'universita e della ricerca/ PT - Journal Article DEP - 20261011 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709201 OTO - NOTNLM OT - apoptosis OT - composites OT - inflammation OT - polymerization COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/11 CRDT- 2026/10/27 10:43 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - polym18202484 [pii] AID - polymers-18-02484 [pii] AID - 10.3390/polym18202484 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 11;18(20):2484. doi: 10.3390/polym18202484. PMID- 42942601 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 11 TI - The Response of Flame-Retardant Polypropylene Composites After Long-Term Exposure to Corrosive Media. LID - 10.3390/polym18202480 [doi] LID - 2480 AB - This study evaluates the response of flame-retardant polypropylene composites after long-term exposure to aqueous and chemically aggressive media. Polypropylene (PP), used as a reference material, and PP-based composites containing 5, 10, 15, 20, and 25 wt.% of a phosphorus-based intumescent flame-retardant additive were immersed in water, NaCl solution, H(2)SO(4) solution, and NaOH solution for up to 200 days. The material behavior was initially assessed by gravimetric measurements, based on the absorption/degradation degree calculated for early, mid, and late exposure intervals. This allowed the evolution of the material-medium interaction to be tracked not only by individual mass variations but also by average trends over the exposure period. The exposed systems were further investigated by thermogravimetric analysis (TGA/DTG) and dynamic mechanical analysis (DMA) to determine whether contact with the selected media modified the thermal degradation profile or the dynamic mechanical response of the composites. The results showed that the incorporated flame-retardant additive increases the interaction of PP with the liquid media, especially at intermediate and high additive contents. TGA/DTG and DMA confirmed that the thermal and viscoelastic behavior of the PP-based systems was maintained after long-term exposure, indicating that the observed medium-induced interactions did not result in substantial deterioration of their bulk thermo-mechanical properties. FAU - Vilcea, Elena-Janina AU - Vilcea EJ AUID- ORCID: 0009-0002-6209-4180 AD - Manufacturing Engineering Department, Faculty of Industrial Engineering and Robotics, National University of Science and Technology POLITEHNICA Bucharest, 060042 Bucharest, Romania. FAU - Balanuca, Brindusa AU - Balanuca B AUID- ORCID: 0000-0001-8600-3795 AD - Department of Organic Chemistry "C. Nenitescu", Faculty of Chemical Engineering and Biotechnology, National University of Science and Technology POLITEHNICA Bucharest, 011061 Bucharest, Romania. AD - Advanced Polymer Materials Group, National University of Science and Technology POLITEHNICA Bucharest, 011061 Bucharest, Romania. FAU - Necolau, Madalina Ioana AU - Necolau MI AUID- ORCID: 0000-0002-1032-9382 AD - Advanced Polymer Materials Group, National University of Science and Technology POLITEHNICA Bucharest, 011061 Bucharest, Romania. FAU - Dijmarescu, Manuela-Roxana AU - Dijmarescu MR AUID- ORCID: 0000-0002-5951-5442 AD - Manufacturing Engineering Department, Faculty of Industrial Engineering and Robotics, National University of Science and Technology POLITEHNICA Bucharest, 060042 Bucharest, Romania. FAU - Ion, Rodica-Mariana AU - Ion RM AUID- ORCID: 0000-0002-9842-3321 AD - Doctoral School of Engineering Sciences, Valahia University of Targoviste, 13 Aleea Sinaia, 130004 Targoviste, Romania. AD - National Institute for Research & Development for Chemistry and Petrochemistry, 202 Splaiul Independentei, 060021 Bucharest, Romania. AD - Academy of Romanian Scientists, 3 Ilfov Street, 030167 Bucharest, Romania. FAU - Toader, Maria-Cristina AU - Toader MC AUID- ORCID: 0000-0002-6139-9321 AD - Quality Engineering and Industrial Technologies Department, Faculty of Industrial Engineering and Robotics, National University of Science and Technology POLITEHNICA Bucharest, 060042 Bucharest, Romania. LA - eng PT - Journal Article DEP - 20261011 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709159 OTO - NOTNLM OT - absorption/degradation degree OT - aqueous and corrosive media OT - halogen-free flame retardant OT - long-term exposure OT - phosphorus-based intumescent flame retardant OT - polypropylene composites COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/11 CRDT- 2026/10/27 10:43 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - polym18202480 [pii] AID - polymers-18-02480 [pii] AID - 10.3390/polym18202480 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 11;18(20):2480. doi: 10.3390/polym18202480. PMID- 42942598 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 10 TI - Devulcanization of Sulfur-Cured EPDM: A Critical Review of Selective Crosslink Scission, Its Measurement, and Continuous Processing. LID - 10.3390/polym18202477 [doi] LID - 2477 AB - Sulfur-cured ethylene-propylene-diene monomer (EPDM) sealing scrap cannot be melt-reprocessed; devulcanization recovers rubber only if it cuts crosslinks selectively. This review separates EPDM-direct findings from tire, natural rubber (NR) and styrene-butadiene-rubber transfers. Metrics have converged on swelling, crosslink density, sol fraction, Horikx selectivity analysis, and a rheological proxy, without a grading standard. Two EPDM studies favored milder temperatures; extrusion selectivity depended jointly on thermal profile and screw speed, and excessive severity can cause re-crosslinking. Thermomechanical and agent-assisted extrusion have run continuously on EPDM in more than one laboratory; one group's agent-assisted devulcanizate met the tensile, compression set and hardness limits of a non-automotive seal specification, the last two only after blending with 25 wt% virgin rubber. In the only direct comparison, ethylidene-norbornene EPDM was much less reactive toward disulfides than NR, which its authors attribute to a denser, more monosulfidic network less prone to main-chain scission. A single EPDM process life cycle assessment (LCA) identifies energy as the main environmental lever. The review contributes maturity classes, scission pathways labeled proposed or demonstrated, and a minimum LCA reporting set. Closing five EPDM-direct gaps (grading standards, green-route data, in-line selectivity sensing, joint throughput-energy-quality reporting, feedstock sorting) would make continuous agent-assisted extrusion a specifiable route. FAU - Nam, Ki-Dong AU - Nam KD AD - Exterior & Closure Materials Development Team, Hyundai Motor Company, Hwaseong-si 18280, Republic of Korea. FAU - Cho, Yeon-Jee AU - Cho YJ AD - Sustainable Materials R & D Center, Korea Automotive Technology Institute, Cheonan-si 31214, Republic of Korea. FAU - Kim, Ho-Dong AU - Kim HD AD - Exterior & Closure Materials Development Team, Hyundai Motor Company, Hwaseong-si 18280, Republic of Korea. FAU - Yoon, Jeong-Hwan AU - Yoon JH AUID- ORCID: 0000-0002-9607-4770 AD - Sustainable Materials R & D Center, Korea Automotive Technology Institute, Cheonan-si 31214, Republic of Korea. LA - eng GR - RS-2026-25543162/Korea Evaluation Institute of Industrial Technology/ GR - 2410019249/Korea Evaluation Institute of Industrial Technology/ PT - Journal Article PT - Review DEP - 20261010 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13708930 OTO - NOTNLM OT - EPDM OT - Horikx analysis OT - crosslink density OT - crosslink scission OT - devulcanization OT - life cycle assessment OT - reactive extrusion OT - reclaiming OT - rubber recycling COIS- Authors Ki-Dong Nam and Ho-Dong Kim were employed by the Exterior & Closure Materials Development Team, Hyundai Motor Company. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:43 PHST- 2026/09/23 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:43 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - polym18202477 [pii] AID - polymers-18-02477 [pii] AID - 10.3390/polym18202477 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 10;18(20):2477. doi: 10.3390/polym18202477. PMID- 42942593 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 10 TI - Effect of Sulfonate-, Phosphonate-, and Lipoate-Functional Modifiers on the Mechanical and Thermal Properties of Polymethyl Methacrylate Denture Base Resin. LID - 10.3390/polym18202472 [doi] LID - 2472 AB - Objective: This study aimed to evaluate the mechanical and thermal properties of heat-polymerized polymethyl methacrylate (PMMA) denture base resin modified with different concentrations of sodium 4-styrenesulfonate (SO), 5,5-bis(diisopropoxyphosphoryl)pentyl methacrylate (PO), and 5,5-bis(diisopropoxyphosphoryl)pentyl lipoate (LIP). Materials and Methods: Heat-polymerized PMMA specimens were divided into a control group and experimental groups modified with 2%, 3%, or 5% SO, PO, or LIP (n = 8 per group). Maximum deflection, flexural strength, and Young's modulus were determined using a three-point bending test. Surface hardness was evaluated using the Vickers hardness test, thermal behavior was assessed by thermogravimetric analysis (TGA), and fracture surfaces were examined by scanning electron microscopy (SEM). Data were analyzed using two-way ANOVA (modifier type x concentration) with Bonferroni-adjusted pairwise comparisons, and comparisons including the control group were performed using Welch's or one-way ANOVA followed by Tamhane's T2 or Bonferroni post hoc tests, depending on the homogeneity of variances (alpha = 0.05). Results: Modifier type significantly affected maximum deflection, flexural strength, Young's modulus, and Vickers hardness of specimens. The LIP-containing groups showed the highest mean deflection values but significantly lower flexural strength, Young's modulus, and hardness than the control group. In contrast, the SO-containing groups generally maintained mechanical properties closer to those of unmodified PMMA. TGA showed that all modifiers altered the thermal degradation profile of PMMA, with PO increasing the main degradation temperature by approximately 40 degrees C. Conclusions: The investigated modifiers produced distinct, modifier-dependent changes in the mechanical and thermal behavior of heat-polymerized PMMA. Although LIP increased deformation capacity, this occurred at the expense of flexural strength and stiffness. SO and PO modifications showed comparatively smaller changes in mechanical properties at selected concentrations. Further studies including direct fracture-toughness, fatigue, and clinically relevant aging assessments are required to determine whether these modifications provide functional advantages for denture base applications. FAU - Erdal, Selda Gokce AU - Erdal SG AUID- ORCID: 0009-0009-5141-9067 AD - Department of Prosthodontics, Faculty of Dentistry, Zonguldak Bulent Ecevit University, 67100 Zonguldak, Turkiye. FAU - Acar, Merve AU - Acar M AUID- ORCID: 0009-0005-3123-7398 AD - Department of Prosthodontics, Faculty of Dentistry, Zonguldak Bulent Ecevit University, 67100 Zonguldak, Turkiye. FAU - Koroglu, Aysegul AU - Koroglu A AUID- ORCID: 0000-0002-0288-6357 AD - Department of Prosthodontics, Faculty of Dentistry, Zonguldak Bulent Ecevit University, 67100 Zonguldak, Turkiye. FAU - Hazer, Baki AU - Hazer B AD - Department of Aircraft Airframe Engine Maintenance, Kapadokya University, 50420 Urgup, Turkiye. AD - Department of Chemistry, Zonguldak Bulent Ecevit University, 67100 Zonguldak, Turkiye. LA - eng PT - Journal Article DEP - 20261010 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709116 OTO - NOTNLM OT - bisphosphonate OT - denture bases OT - flexural strength OT - lipoate OT - poly(methyl methacrylate) OT - sodium 4-styrenesulfonate OT - thermal stability COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:42 PHST- 2026/09/06 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - polym18202472 [pii] AID - polymers-18-02472 [pii] AID - 10.3390/polym18202472 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 10;18(20):2472. doi: 10.3390/polym18202472. PMID- 42942590 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 10 TI - Per-Layer UV Exposure-Dependent Cytotoxicity of 3D-Printed Aligner Resins and Estrogen-like Activity Screening. LID - 10.3390/polym18202469 [doi] LID - 2469 AB - Per-layer UV exposure during 3D printing may influence polymerization and the biological response of photocurable aligner resins. This study evaluated exposure-dependent cytotoxicity of Tera Harz TC-85 and NextDent Ortho Flex and performed an exploratory screening of selected resin-specific printing conditions for ER-selective estrogen-like responses. TC-85 was printed at 2.0, 2.4, 3.0, and 4.0 s per layer, and Ortho Flex at 5.0, 6.0, 7.0, and 8.0 s per layer. The resin-specific per-layer UV exposure ranges were selected based on our previous physicochemical characterization of the same materials and printing system. Extracts were prepared according to ISO 10993-12. Cytotoxicity was assessed in human gingival fibroblasts using the MTT assay, while selected conditions (TC-85, 2.4 s; Ortho Flex, 7.0 s) were evaluated in estrogen receptor (ER)-positive MCF-7 and ER-negative MDA-MB-231 cells as an exploratory screening assay rather than a direct test of ER activation. For both resins, per-layer exposure condition, extract concentration, and their interaction significantly affected the normalized MTT response. At 50% extract concentration, normalized MTT responses ranged from 89.2% to 111.3%, whereas Ortho Flex ranged from 8.8% to 76.8%, with the 8.0 s condition being the only Ortho Flex condition above the 70% cytotoxicity threshold. Ortho Flex showed pronounced cytotoxicity in concentrated extracts, particularly at shorter exposure times, whereas TC-85 showed a less monotonic exposure-dependent pattern. In the exploratory screening, the 6.25% Ortho Flex extract produced a normalized MCF-7 MTT response of 129.1%, but this was comparable with the corresponding saline vehicle response of 137.0% and was not statistically greater than the response in ER-negative MDA-MB-231 cells. No statistically supported ER-selective response was detected for either tested resin under the specific screening conditions; however, exposure-time dependence of this response was not evaluated. Per-layer UV exposure therefore influenced the HGF-1 MTT response in a resin- and concentration-dependent manner. For dental laboratory practice, these findings support the use of resin-specific, validated printing parameters rather than transferring exposure settings between materials; deviations from manufacturer-specified processing conditions should be independently validated before clinical use. FAU - Simunovic, Luka AU - Simunovic L AUID- ORCID: 0000-0003-2848-6041 AD - Department of Orthodontics, School of Dental Medicine, University of Zagreb, 10000 Zagreb, Croatia. FAU - Maric, Antun Jakob AU - Maric AJ AUID- ORCID: 0009-0008-2099-3237 AD - Faculty of Mechanical Engineering and Naval Architecture, University of Zagreb, 10000 Zagreb, Croatia. FAU - Mestrovic, Senka AU - Mestrovic S AUID- ORCID: 0000-0003-3224-3579 AD - Department of Orthodontics, School of Dental Medicine, University of Zagreb, 10000 Zagreb, Croatia. LA - eng GR - SFZG-11-2025_NAT-DM/University of Zagreb/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709321 OTO - NOTNLM OT - 3D printing OT - clear aligners OT - cytotoxicity OT - estrogen-like activity OT - orthodontic polymers OT - per-layer UV exposure OT - photopolymerization COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:42 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - polym18202469 [pii] AID - polymers-18-02469 [pii] AID - 10.3390/polym18202469 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 10;18(20):2469. doi: 10.3390/polym18202469. PMID- 42942588 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 9 TI - Sustainable Polymers as System-Dependent Technologies: A Critical Engineering Assessment of LCA Evidence, Circular Compatibility, and Scalability Constraints. LID - 10.3390/polym18202467 [doi] LID - 2467 AB - The transition toward sustainable polymer systems is frequently framed as a material substitution challenge. However, the environmental performance of bio-based, biodegradable, and recyclable polymers is not an intrinsic property of feedstock origin but a system-dependent outcome shaped by energy inputs, infrastructure compatibility, economic feasibility, and governance alignment. This review provides a critical engineering assessment of sustainable polymer technologies based on systematic evidence screening (n = 193), bibliometric analysis, life cycle assessment (LCA) synthesis, techno-economic indicators, end-of-life scenario interpretation, and a multi-criteria evidence synthesis based on source-linked quantitative indicators and categorical evidence. To avoid treating these components as parallel but disconnected analyses, the synthesis explicitly links bibliometric clusters with LCA, TEA, and infrastructure evidence categories through an evidence-gap mapping matrix and confidence grading scheme. The findings indicate that bio-based polymers, such as PLA, PHA, and PBS, may achieve reduced greenhouse gas emissions under optimized production conditions; however, these advantages are highly sensitive to electricity carbon intensity, land-use assumptions, and end-of-life infrastructure. Quantitative LCA evidence indicates that polylactic acid (PLA) may achieve greenhouse gas emission reductions in the range of approximately 25-55% compared to conventional PET under low-carbon energy scenarios (1.3-3.0 vs. 2.0-3.5 kg CO(2)e/kg), while polyhydroxyalkanoates (PHA) show reductions of 10-40%, depending on fermentation efficiency and feedstock selection. However, these benefits can diminish or reverse under fossil-intensive energy mixes or when land-use change impacts are included, reinforcing the system-dependent nature of sustainability performance. Recyclability within established material streams frequently provides more stable long-term performance than biodegradable alternatives deployed without controlled composting systems. Evidence-strength analysis reveals moderate confidence in carbon footprint comparisons, weak-to-moderate confidence in techno-economic scalability claims, and conditional confidence in infrastructure-dependent outcomes. The results demonstrate that sustainable polymers are system-dependent technologies rather than inherently sustainable materials. Future engineering research must prioritize integrated LCA-TEA modeling, infrastructure-aligned material design, scenario-based end-of-life assessment, and harmonized sustainability reporting standards to enable scalable and verifiable circular polymer systems. FAU - Lazar, Sergiu AU - Lazar S AUID- ORCID: 0000-0002-4945-4586 AD - Department of Industrial Engineering and Management, Faculty of Engineering, Lucian Blaga University of Sibiu, 550024 Sibiu, Romania. FAU - Dobrota, Dan AU - Dobrota D AUID- ORCID: 0000-0002-2202-7701 AD - Department of Industrial Engineering and Management, Faculty of Engineering, Lucian Blaga University of Sibiu, 550024 Sibiu, Romania. FAU - Rotaru, Ionela Magdalena AU - Rotaru IM AD - S.C. Fusion Romania S.R:L, Stefan cel Mare Street, no. 152-154, 550321 Sibiu, Romania. FAU - Dobrota, Gabriela AU - Dobrota G AD - Faculty of Economics, "Constantin Brancusi" University of Targu Jiu, 210185 Targu Jiu, Romania. LA - eng PT - Journal Article PT - Review DEP - 20261009 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13708781 OTO - NOTNLM OT - bioplastics OT - circular economy OT - green plastics OT - life cycle assessment OT - polymers engineering OT - renewable polymers OT - sustainable polymers COIS- Ionela Magdalena Rotaru was employed by S.C. Fusion Romania S.R.L. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:42 PHST- 2026/08/24 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - polym18202467 [pii] AID - polymers-18-02467 [pii] AID - 10.3390/polym18202467 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 9;18(20):2467. doi: 10.3390/polym18202467. PMID- 42942585 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 9 TI - Architecture-Specific Performance of Silica and MWCNT Modified Hybrid Epoxy Laminates Under Mechanical Loading and Fracture. LID - 10.3390/polym18202463 [doi] LID - 2463 AB - Hybrid fiber-reinforced epoxy laminates integrate macroscale fiber architecture with nanoscale matrix modification. The effects of nanofillers cannot be isolated from laminate architecture when both variables are altered simultaneously. This study independently evaluated silica-modified Kevlar/basalt/alkali-resistant glass (K/B/ARG), silica-modified Kevlar/carbon/alkali-resistant glass (K/C/ARG), and multi-walled carbon nanotube (MWCNT)-modified Kevlar/glass/carbon (K/G/C) laminates. Each laminate family incorporated 0, 1.5, 3.0, 4.5, or 6.0 wt.% nanofiller. Mechanical performance was assessed using tensile, flexural, interlaminar-shear, quasi-static puncture, punch-shear, impact, and energy-absorption tests. Scanning electron microscopy (SEM) was employed to examine fracture morphology, and selected K/C/ARG laminates underwent thermogravimetric analysis (TGA/DTG). For each architecture, 4.5 wt.% nanofiller yielded the highest mean values for most measured responses. At this concentration, K/B/ARG achieved 167.93 +/- 5.71 MPa tensile strength, 112.09 +/- 3.70 MPa puncture strength, and 1598.1 +/- 73.5 J/m Izod impact strength. K/C/ARG achieved 171.95 +/- 5.50 MPa tensile strength, 7710 +/- 285.3 N puncture load, and 1813.6 +/- 79.8 J/m Izod impact strength. K/G/C achieved 215.58 +/- 7.76 MPa tensile strength, 28.49 +/- 1.20 MPa interlaminar-shear strength, 110.80 +/- 5.54 MPa punch-shear strength, and 15.45 +/- 0.77 J net energy absorption. Several matrix-sensitive responses declined at 6.0 wt.%, while K/G/C flexural strength remained nearly constant. SEM revealed greater matrix retention and more uniform damage distribution at intermediate nanofiller loading but clustered weak regions at 6.0 wt.%. Single-run TGA indicated a higher principal DTG temperature and greater residue for the 4.5 wt.% K/C/ARG formulation; however, replication is necessary. The convolutional neural network (CNN) analysis was constrained by the limited number of formulation levels, target leakage, and the absence of independent validation. Consequently, 4.5 wt.% is identified as the best-performing tested concentration within each architecture, rather than a universal optimum. Additional durability, fire, and structural-scale testing is required before these laminates can be considered for building design, resilient infrastructure, or sustainable construction. FAU - Bashir, Muhammad Tariq AU - Bashir MT AUID- ORCID: 0000-0002-8769-9875 AD - Department of Civil Engineering, CECOS University of IT & Emerging Sciences, Peshawar 25000, Pakistan. AD - Faculty of Engineering and Quantity Surveying (FEQS), INTI International University, Persiaran Perdana BBN, Nilai 71800, Malaysia. FAU - Naseem, Khawaja Atif AU - Naseem KA AD - Traffic Safety Engineer, Arkansas Department of Transportation, 10324 I-30, Little Rock, AR 72209, USA. FAU - Khan, Atif AU - Khan A AUID- ORCID: 0009-0006-0230-4229 AD - Department of Civil and Environmental Engineering, University of Louisiana at Lafayette, Lafayette, LA 70503, USA. FAU - Sadiq, Muhammad Rehan AU - Sadiq MR AD - STV Inc., 5750 Genesis Court, Suite 200, Frisco, TX 75034, USA. FAU - Khan, Md Munir Hayet AU - Khan MMH AUID- ORCID: 0000-0002-0947-5008 AD - Faculty of Engineering and Quantity Surveying (FEQS), INTI International University, Persiaran Perdana BBN, Nilai 71800, Malaysia. FAU - Sikandar, Muhammad Ali AU - Sikandar MA AUID- ORCID: 0000-0003-0043-8216 AD - Department of Civil Engineering, CECOS University of IT & Emerging Sciences, Peshawar 25000, Pakistan. AD - Faculty of Engineering and Quantity Surveying (FEQS), INTI International University, Persiaran Perdana BBN, Nilai 71800, Malaysia. LA - eng PT - Journal Article DEP - 20261009 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13709037 OTO - NOTNLM OT - architecture-specific response OT - exploratory machine learning OT - fracture morphology OT - hybrid epoxy laminates OT - multi-walled carbon nanotubes OT - punch shear OT - silica nanoparticles OT - sustainable buildings OT - uncertainty COIS- Author Muhammad Rehan Sadiq was employed by the company STV Inc. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:42 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - polym18202463 [pii] AID - polymers-18-02463 [pii] AID - 10.3390/polym18202463 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 9;18(20):2463. doi: 10.3390/polym18202463. PMID- 42942580 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2073-4360 (Electronic) IS - 2073-4360 (Linking) VI - 18 IP - 20 DP - 2026 Oct 9 TI - Effect of ChCl:Glycolic Acid Molar Ratio and Plant Extract Incorporation on the Structure-Property Relationships of Chitosan Active Packaging Films. LID - 10.3390/polym18202459 [doi] LID - 2459 AB - The influence of choline chloride:glycolic acid (ChCl:GA) stoichiometry and plant extract incorporation on the properties of chitosan-based active packaging films was investigated. A deep eutectic solvent with a ChCl:GA molar ratio of 1:2 (DES_GA2) was used both as a chitosan plasticizer and an extraction medium for neem and hawthorn compounds. Films containing 44-70 wt% DES_GA2 were characterized to establish composition-structure-property relationships. The 50 wt% formulation showed a favorable balance between mechanical flexibility and water vapor barrier performance and was selected for plant extract incorporation. Neem extract showed higher radical-scavenging activity than hawthorn despite its lower total phenolic content. Extract incorporation enhanced UV shielding and reduced water vapor permeability, with the hawthorn-containing film showing an approximately 41% reduction in WVP. Extract-containing films retained antioxidant functionality and showed antibacterial activity against Escherichia coli. Comparison with the previously investigated ChCl 1:3 system revealed stoichiometry-dependent behavior. At 50 wt% NaDES, increasing the ChCl:GA molar ratio to 1:2 increased elongation at break from 90.5% to 120.1% and reduced Young's modulus from 131.0 to 48.0 MPa. Comparison of neem-loaded films based on different NaDES systems further demonstrated the influence of the NaDES composition. These results identify the NaDES composition and botanical source as relevant formulation variables for tailoring CS-NaDES active packaging films. FAU - Stanescu, Paul AU - Stanescu P AUID- ORCID: 0000-0002-5861-1934 AD - Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania. FAU - Gavrila, Adina AU - Gavrila A AUID- ORCID: 0000-0002-0369-594X AD - Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania. FAU - Isopencu, Gabriela AU - Isopencu G AUID- ORCID: 0000-0002-0763-540X AD - Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania. FAU - Motelica, Ludmila AU - Motelica L AUID- ORCID: 0000-0002-3371-4136 AD - Research Center for Advanced Materials, Products and Processes, National University of Science and Technology POLITEHNICA Bucharest, 313 Splaiul Independentei, 060042 Bucharest, Romania. AD - National Research Center for Micro and Nanomaterials, National University of Science and Technology POLITEHNICA Bucharest, 313 Spl. Independentei, 060042 Bucharest, Romania. FAU - Nicoara, Adrian Ionut AU - Nicoara AI AUID- ORCID: 0000-0002-4133-7377 AD - Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania. AD - National Research Center for Micro and Nanomaterials, National University of Science and Technology POLITEHNICA Bucharest, 313 Spl. Independentei, 060042 Bucharest, Romania. FAU - Sima, Sergiu AU - Sima S AUID- ORCID: 0000-0002-4789-7287 AD - Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania. FAU - Bumbaru Dandes, Mihaela AU - Bumbaru Dandes M AD - Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania. FAU - Ciocirlan, Oana AU - Ciocirlan O AD - Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania. FAU - Oprea, Ovidiu-Cristian AU - Oprea OC AUID- ORCID: 0000-0002-8145-1094 AD - Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, 1-7 Gh. Polizu Street, 011061 Bucharest, Romania. AD - National Research Center for Micro and Nanomaterials, National University of Science and Technology POLITEHNICA Bucharest, 313 Spl. Independentei, 060042 Bucharest, Romania. AD - Academy of Romanian Scientists, 3 Ilfov Street, 050044 Bucharest, Romania. LA - eng GR - Polytechnic University of Bucharest/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Polymers (Basel) JT - Polymers JID - 101545357 PMC - PMC13708844 OTO - NOTNLM OT - NaDES OT - active packaging OT - antioxidant activity OT - chitosan OT - choline chloride OT - glycolic acid OT - plant extracts COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:42 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - polym18202459 [pii] AID - polymers-18-02459 [pii] AID - 10.3390/polym18202459 [doi] PST - epublish SO - Polymers (Basel). 2026 Oct 9;18(20):2459. doi: 10.3390/polym18202459. PMID- 42942572 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 21 TI - Development of a Clinical Prediction Model for Systemic Therapy Escalation in Oral Lichen Planus: A Two-Center Retrospective Cohort Study. LID - 10.3390/medicina62102041 [doi] LID - 2041 AB - Background and Objectives: Oral lichen planus (OLP) is a chronic immune-mediated disorder in which the need for systemic therapy remains difficult to predict using standardized clinical criteria. This retrospective two-center cohort study aimed to develop a multivariable clinical prediction model for systemic therapy escalation in patients with histopathologically confirmed OLP. Material and Methods: Medical records of 141 adult patients treated between 2022 and 2025 were reviewed. Baseline demographic, clinical, and laboratory variables were analyzed using univariate analyses, followed by multivariable logistic regression with backward stepwise selection and Firth penalized likelihood estimation. Discrimination was assessed by the area under the ROC curve (AUC), and internal validation was performed by bootstrap resampling (1000 samples) with repetition of the full selection procedure. Results: Four independent predictors of systemic therapy were identified: concomitant cutaneous lichen planus (penalized odds ratio (OR) 13.96, 95% CI 3.94-55.84), autoimmune thyroiditis (OR 12.67, 3.26-54.88), genital lichen planus (OR 10.21, 2.36-51.57) and oral Candida superinfection (OR 4.48, 1.10-18.82). The apparent AUC was 0.889 (95% CI 0.801-0.976); after bootstrap internal validation, the optimism-corrected AUC was 0.84 and the calibration slope 0.73, indicating moderate overfitting. Candida superinfection was the least stable predictor (selected in 43% of bootstrap samples). A prognostic risk matrix was developed to estimate the probability of systemic therapy escalation according to different combinations of predictors. Conclusions: The identified clinical factors and derived risk matrix may provide a preliminary framework for risk stratification and individualized therapeutic decision-making in patients with OLP. However, given the retrospective design and limited number of outcome events, these findings should be interpreted cautiously. Prospective external validation in larger independent cohorts is required before the model can be considered for routine clinical use. FAU - Toader, Mihaela Paula AU - Toader MP AUID- ORCID: 0000-0002-9838-836X AD - Department of Surgicals, Faculty of Dental Medicine, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. AD - Dermatology Clinic, Railways University Hospital, 700506 Iasi, Romania. FAU - Popa, Cristina AU - Popa C AUID- ORCID: 0000-0001-8739-4679 AD - Department of Surgicals, Faculty of Dental Medicine, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. AD - Ambulatory of Oral and Maxillofacial Surgery of the "St. Spiridon" County Clinical Emergency Hospital, 700106 Iasi, Romania. FAU - Colac-Botoc, Cristina AU - Colac-Botoc C AD - Dermatology Clinic, Railways University Hospital, 700506 Iasi, Romania. FAU - Sciuca, Ana Maria AU - Sciuca AM AD - Department of Surgicals, Faculty of Dental Medicine, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. AD - Ambulatory of Oral and Maxillofacial Surgery of the "St. Spiridon" County Clinical Emergency Hospital, 700106 Iasi, Romania. FAU - Vicovan, Anastasia AU - Vicovan A AD - Dermatology Clinic, Railways University Hospital, 700506 Iasi, Romania. FAU - Branisteanu, Daciana Elena AU - Branisteanu DE AD - Dermatology Clinic, Railways University Hospital, 700506 Iasi, Romania. AD - Department of Dermatology, Faculty of Medicine, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. LA - eng PT - Journal Article PT - Multicenter Study PT - Observational Study DEP - 20261021 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Adult MH - Aged MH - Female MH - Humans MH - Male MH - Middle Aged MH - Cohort Studies MH - *Lichen Planus, Oral/drug therapy MH - Logistic Models MH - Prediction Algorithms MH - Retrospective Studies MH - ROC Curve PMC - PMC13707202 OTO - NOTNLM OT - oral lichen planus OT - prediction model OT - systemic therapy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/21 CRDT- 2026/10/27 10:42 PHST- 2026/08/02 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - medicina62102041 [pii] AID - medicina-62-02041 [pii] AID - 10.3390/medicina62102041 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 21;62(10):2041. doi: 10.3390/medicina62102041. PMID- 42942568 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 21 TI - From Evidence to Implementation: FIB-4-Based Risk Stratification for Advanced Liver Disease in Saudi Primary Care. LID - 10.3390/medicina62102037 [doi] LID - 2037 AB - Metabolic dysfunction-associated steatotic liver disease (MASLD) is increasingly common and poses a growing challenge for health systems, because most affected people are managed outside specialist liver services. The clinically important task is not simply to identify hepatic steatosis, but to find the smaller subgroup with clinically significant fibrosis who face the greatest risk of liver-related complications and who may now be eligible for liver-directed pharmacotherapy. The fibrosis-4 index (FIB-4), calculated from age, aspartate aminotransferase, alanine aminotransferase and platelet count, has emerged as a pragmatic first-line tool for fibrosis risk stratification. Contemporary guidance recommends a stepwise approach in at-risk populations, with FIB-4 followed by second-line non-invasive testing, most commonly vibration-controlled transient elastography (VCTE) or the Enhanced Liver Fibrosis (ELF) test. The September 2026 Global NASH/MASH Council consensus consolidates this fibrosis-centred, non-invasive-test-driven model further and links second-line test thresholds to treatment initiation and response monitoring in non-cirrhotic MASH with F2-F3 fibrosis. Saudi Arabia is a particularly relevant setting given its substantial cardiometabolic burden and emerging evidence of a high MASLD prevalence. Recent Saudi data include a multicentre primary-care cohort reporting an age- and sex-adjusted MASLD prevalence of 34.2%, a real-world primary-care study demonstrating intermediate or high FIB-4 risk among metabolically high-risk patients despite normal liver enzymes, and a Saudi consensus incorporating FIB-4 into local risk stratification. Important implementation questions remain, however, including target populations, age-related interpretation, laboratory readiness, second-line testing capacity, referral pathways, treatment access, repeat assessment, electronic health-record integration and cost-effectiveness. This narrative review traces the evolution of FIB-4 from a prognostic marker to a primary-care triage tool, critically examines contemporary international and Saudi evidence, and proposes a context-sensitive framework and staged research agenda for FIB-4-based risk stratification in Saudi primary care. FAU - Alenezi, Yusef Muhana AU - Alenezi YM AUID- ORCID: 0000-0003-0468-2275 AD - Family and Community Department, Faculty of Medicine, Northern Border University, Arar 91431, Saudi Arabia. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - EC 2.6.1.1 (Aspartate Aminotransferases) RN - EC 2.6.1.2 (Alanine Transaminase) SB - IM MH - Humans MH - Saudi Arabia/epidemiology MH - Primary Health Care MH - Risk Assessment/methods MH - *Liver Cirrhosis/diagnosis MH - Aspartate Aminotransferases/blood/analysis MH - Alanine Transaminase/blood/analysis PMC - PMC13706892 OTO - NOTNLM OT - FIB-4 OT - MASLD OT - Saudi Arabia OT - case-finding OT - liver fibrosis OT - non-invasive tests OT - primary care OT - risk stratification COIS- The author declares no conflicts of interest relevant to this manuscript. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/21 CRDT- 2026/10/27 10:42 PHST- 2026/09/27 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - medicina62102037 [pii] AID - medicina-62-02037 [pii] AID - 10.3390/medicina62102037 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 21;62(10):2037. doi: 10.3390/medicina62102037. PMID- 42942564 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 20 TI - Toward Precision and Less Invasive Surgery in Thyroid, Breast, and Endocrine Diseases: Current Advances and Future Directions. LID - 10.3390/medicina62102033 [doi] LID - 2033 AB - Surgery remains a cornerstone of treatment for many thyroid, breast, and other endocrine diseases, but its goals have evolved beyond effective disease removal alone [...]. FAU - Pano, Irida AU - Pano I AD - Department of Clinical Subject, University of Medicine Tirana, 1005 Tirana, Albania. FAU - Mulita, Francesk AU - Mulita F AUID- ORCID: 0000-0001-7198-2628 AD - Second Department of Surgery, Medical School, Democritus University of Thrace, 68100 Alexandroupolis, Greece. FAU - Leivaditis, Vasileios AU - Leivaditis V AUID- ORCID: 0000-0001-8692-0059 AD - Department of Cardiothoracic and Vascular Surgery, Westpfalz Klinikum, 67655 Kaiserslautern, Germany. LA - eng PT - Editorial DEP - 20261020 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - *Thyroid Diseases/surgery MH - *Endocrine System Diseases/surgery MH - Female MH - *Breast Diseases/surgery MH - *Minimally Invasive Surgical Procedures/methods/trends PMC - PMC13707247 COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/20 CRDT- 2026/10/27 10:42 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - medicina62102033 [pii] AID - medicina-62-02033 [pii] AID - 10.3390/medicina62102033 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 20;62(10):2033. doi: 10.3390/medicina62102033. PMID- 42942563 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 20 TI - Long-Term Effects of Paced Heart Rate on Central Blood Pressure in Patients with Sinus Node Dysfunction and Hypertension: A Randomised Crossover Study. LID - 10.3390/medicina62102032 [doi] LID - 2032 AB - Background and Objectives: Prior cardiac pacemaker studies examining the relationship between heart rate and central (aortic) blood pressure (BP) have predominantly used acute designs, limiting generalisability to long-term settings. The aim of this study was to assess the long-term effects of two clinically realistic pacing rate strategies on the central BP profile in permanently paced sinus node dysfunction (SND) patients in sinus rhythm and on antihypertensive medication. Materials and Methods: In this prospective, single-centre, randomised, single-blind, exploratory crossover clinical trial, the study participants completed two eight-week intervention periods (DDD 55 and 75 bpm) separated by a two-week washout. Office peripheral and central haemodynamics were measured at baseline and at the end of each period non-invasively with an oscillometric cuff-based device (SphygmoCor XCEL). Results: The study included 22 subjects (median age 76 (IQR 73-78) years, 16 women). At DDD 55 bpm, central systolic BP (primary outcome) was higher than at DDD 75 bpm (mean 127.8 +/- 13.4 and 121.3 +/- 10.6 mmHg, respectively, p = 0.04), whereas systolic BP amplification (peripheral systolic BP-central systolic BP) and pulse pressure (PP) amplification (peripheral PP/central PP) were lower (systolic BP amplification: mean 8.5 +/- 3.2 and 10.1 +/- 2.9 mmHg, respectively, p = 0.02; PP amplification: mean 1.2 +/- 0.1 and 1.3 +/- 0.1, respectively, p < 0.01). Conclusions: Long-term pacing at a moderately accelerated physiological rate may be associated with lower central systolic BP compared with pacing at a slightly bradycardic rate, in older permanently paced SND patients with hypertension. FAU - Joonsalu, Tuuli AU - Joonsalu T AD - Department of Cardiology, Institute of Clinical Medicine, University of Tartu, L. Puusepa 8, 50406 Tartu, Estonia. AD - Heart Clinic, Tartu University Hospital, L. Puusepa 8, 50406 Tartu, Estonia. FAU - Heinsar, Silver AU - Heinsar S AUID- ORCID: 0000-0002-5004-4816 AD - Department of Cardiology, Institute of Clinical Medicine, University of Tartu, L. Puusepa 8, 50406 Tartu, Estonia. AD - Centre of Cardiology, North Estonia Medical Centre, J. Sutiste Tee 19, 13419 Tallinn, Estonia. AD - Critical Care Research Group, Institute for Molecular Bioscience, The University of Queensland, 306 Carmody Road, Brisbane 4072, Australia. FAU - Serg, Martin AU - Serg M AUID- ORCID: 0009-0006-0766-7600 AD - Department of Cardiology, Institute of Clinical Medicine, University of Tartu, L. Puusepa 8, 50406 Tartu, Estonia. AD - Centre of Cardiology, North Estonia Medical Centre, J. Sutiste Tee 19, 13419 Tallinn, Estonia. FAU - Kals, Mart AU - Kals M AUID- ORCID: 0000-0003-2090-8780 AD - Estonian Genome Center, Institute of Genomics, University of Tartu, Riia 23b/2, 51010 Tartu, Estonia. AD - Lifeyear, Valukoja 10, 11415 Tallinn, Estonia. FAU - Voitk, Juri AU - Voitk J AD - Centre of Cardiology, North Estonia Medical Centre, J. Sutiste Tee 19, 13419 Tallinn, Estonia. FAU - Zilmer, Mihkel AU - Zilmer M AD - Department of Biochemistry, Institute of Biomedicine and Translational Medicine, Centre of Excellence for Genomics and Translational Medicine, University of Tartu, Ravila 19, 50411 Tartu, Estonia. FAU - Eha, Jaan AU - Eha J AUID- ORCID: 0000-0001-5049-4852 AD - Department of Cardiology, Institute of Clinical Medicine, University of Tartu, L. Puusepa 8, 50406 Tartu, Estonia. AD - Heart Clinic, Tartu University Hospital, L. Puusepa 8, 50406 Tartu, Estonia. FAU - Kampus, Priit AU - Kampus P AUID- ORCID: 0009-0007-0552-0514 AD - Department of Cardiology, Institute of Clinical Medicine, University of Tartu, L. Puusepa 8, 50406 Tartu, Estonia. AD - Centre of Cardiology, North Estonia Medical Centre, J. Sutiste Tee 19, 13419 Tallinn, Estonia. LA - eng GR - PRG 2078/Estonian Research Council/ GR - PSG 1216/Estonian Research Council/ GR - MOB3JD1203/Estonian Research Council/ PT - Journal Article PT - Randomized Controlled Trial DEP - 20261020 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - Female MH - Cross-Over Studies MH - *Heart Rate/physiology MH - Prospective Studies MH - Male MH - *Blood Pressure/physiology MH - *Hypertension/physiopathology/complications MH - Single-Blind Method MH - *Sick Sinus Syndrome/physiopathology/therapy/complications MH - Aged PMC - PMC13706900 OTO - NOTNLM OT - blood pressure OT - heart rate OT - hypertension OT - pacemaker OT - pulse wave analysis OT - sick sinus syndrome OT - sinus node dysfunction COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/20 CRDT- 2026/10/27 10:42 PHST- 2026/08/26 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - medicina62102032 [pii] AID - medicina-62-02032 [pii] AID - 10.3390/medicina62102032 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 20;62(10):2032. doi: 10.3390/medicina62102032. PMID- 42942560 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 20 TI - Safety Profile and Adverse Event Management in Flexible Endoscopic Treatment of Zenker's Diverticulum: A Systematic Review. LID - 10.3390/medicina62102029 [doi] LID - 2029 AB - Background and Objectives: Flexible endoscopic septal division (FESD) and third-space techniques represent first-line treatments for symptomatic Zenker's diverticulum (ZD). While technical and clinical success rates are well established, data regarding adverse events (AEs) and their clinical management remain fragmented in recent literature. This systematic review aimed to evaluate the incidence, typology, and therapeutic strategies for AEs associated with flexible endoscopic ZD treatments. Materials and Methods: A systematic search of PubMed/MEDLINE, Embase, and Scopus was conducted up to July 2026 according to PRISMA guidelines. Studies reporting adult patients undergoing flexible endoscopic interventions for ZD reporting data on complication rates and management were included. Methodological quality was evaluated using the MINORS index. Results: Sixty studies were evaluated, comprising 53 cohort series (64 cohorts, 3172 patients, 3266 procedures: 2119 FESD and 1147 third-space procedures) for pooled quantitative incidence estimation, and 7 single-case reports retained for qualitative complication mapping. Bleeding occurred in 4.2% of cases (5.9% in FESD vs. 1.1% in third-space techniques), with most events occurring intraoperatively and resolving entirely through endoscopic hemostasis. Perforation occurred in 3.2% of procedures (3.9% in FESD vs. 1.8% in third-space series), while mucosal leaks were reported in 0.6% (0.2% in FESD vs. 1.5% in third-space). Severe infectious sequelae, such as abscesses (0.2%) and acute mediastinitis (0.1%), were rare. Conservative measures and endoscopic salvage modalities successfully treated transmural defects, with surgical conversion required in only three perforation cases (<0.1%). Isolated subcutaneous emphysema or pneumomediastinum occurred in 1.3% of cases and resolved uneventfully under conservative observation. Direct procedure-related mortality was 0.03% (1/3266). Conclusions: Flexible endoscopic management of ZD demonstrates a high safety profile with minimal procedural mortality. Complication management has shifted from surgical rescue to conservative protocols and versatile endoscopic salvage. Standardized AE grading and ongoing randomized trials are required to refine procedural selection. FAU - Gualandi, Noemi AU - Gualandi N AUID- ORCID: 0009-0006-3352-5008 AD - Gastroenterology and Digestive Endoscopy Unit, AUSL Modena, 41012 Carpi, Italy. FAU - Bonura, Giuliano Francesco AU - Bonura GF AD - Gastroenterology and Digestive Endoscopy Unit, AUSL Modena, 41012 Carpi, Italy. FAU - Bertoncini, Giulia AU - Bertoncini G AD - Gastroenterology and Digestive Endoscopy Unit, AUSL Modena, 41012 Carpi, Italy. FAU - Frassanito, Gabriella AU - Frassanito G AD - Gastroenterology and Digestive Endoscopy Unit, AUSL Modena, 41012 Carpi, Italy. FAU - Gabbani, Tommaso AU - Gabbani T AUID- ORCID: 0000-0001-8457-2994 AD - Gastroenterology and Digestive Endoscopy Unit, AUSL Modena, 41012 Carpi, Italy. FAU - Maida, Marcello AU - Maida M AUID- ORCID: 0000-0002-4992-9289 AD - Department of Medicine and Surgery, University of Enna 'Kore', 94100 Sicily, Italy. AD - Gastroenterology, Umberto I Hospital, Enna 94100, Italy. FAU - Bona, Davide AU - Bona D AUID- ORCID: 0000-0003-4501-1187 AD - Division of Surgery, Department of Biomedical Sciences for Health, IRCCS Galeazzi Hospital, University of Milan, 20157 Milan, Italy. FAU - Cortegoso Valdivia, Pablo AU - Cortegoso Valdivia P AUID- ORCID: 0000-0002-8793-5890 AD - Gastroenterology and Endoscopy Unit, University Hospital of Parma, University of Parma, 43126 Parma, Italy. AD - Department of Clinical Research, University of Southern Denmark, 5230 Odense, Denmark. AD - Surgical Research Unit, Odense University Hospital, 5000 Odense, Denmark. FAU - Soriani, Paola AU - Soriani P AD - Gastroenterology and Digestive Endoscopy Unit, AUSL Modena, 41012 Carpi, Italy. FAU - Manno, Mauro AU - Manno M AUID- ORCID: 0000-0002-7854-1080 AD - Gastroenterology and Digestive Endoscopy Unit, AUSL Modena, 41012 Carpi, Italy. LA - eng PT - Journal Article PT - Review PT - Systematic Review DEP - 20261020 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - *Zenker Diverticulum/surgery MH - *Esophagoscopy/adverse effects/methods MH - *Postoperative Complications/epidemiology/therapy MH - Female PMC - PMC13707217 OTO - NOTNLM OT - Z-POEM OT - Zenker's diverticulum OT - adverse events OT - flexible endoscopic septotomy OT - systematic review COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/20 CRDT- 2026/10/27 10:42 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - medicina62102029 [pii] AID - medicina-62-02029 [pii] AID - 10.3390/medicina62102029 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 20;62(10):2029. doi: 10.3390/medicina62102029. PMID- 42942558 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 20 TI - Development and Prospective Internal-Validation Protocol of the SpinePROTECT Score: A Digital Biopsychosocial Risk Stratification Framework for Spine Surgery. LID - 10.3390/medicina62102027 [doi] LID - 2027 AB - Background and Objectives: Postoperative recovery after spine surgery remains heterogeneous, and conventional perioperative risk assessment insufficiently represents psychosocial determinants. We developed the preliminary SpinePROTECT Score within a digital biopsychosocial framework intended to support structured preoperative risk stratification. Materials and Methods: Framework development integrated a structured narrative evidence review, evidence from three complementary institutional research programs, predefined candidate-predictor criteria, expert-informed preliminary weighting, and translation into a modular digital platform. The protocol defines a prospective model-development and internal-validation cohort of 300 adults undergoing elective primary or revision surgery for spinal disorders, with follow-up at 6 weeks, 3 months, and 12 months. The study was prospectively registered in the German Clinical Trials Register on 8 September 2026 (DRKS00041544) following ethics approval on 28 August 2026 (protocol code 151/26). At prospective registration, recruitment was scheduled to begin on 10 September 2026; the present protocol article reports no prospective outcome data. Results: The development process yielded three complementary domains: psychological vulnerability, clinical burden, and procedural invasiveness. The preliminary framework includes Type-D personality, depression, anxiety, pain catastrophizing, fear-avoidance beliefs, baseline disability, symptom duration, opioid use, revision status, and surgical trauma. Digital implementation enables electronic questionnaire acquisition, automated calculation of the preliminary SpinePROTECT Score, standardized reports, longitudinal follow-up, and structured research-data capture. Predictive performance is not reported in this protocol article. Conclusions: The SpinePROTECT framework provides a transparent methodological and digital foundation for biopsychosocial risk stratification in spine surgery. The planned prospective study will estimate model coefficients and determine discrimination, calibration, internal validity, and clinical utility before the SpinePROTECT Score is considered for outcome prediction or treatment guidance. FAU - Riediger, Christian AU - Riediger C AUID- ORCID: 0000-0002-2336-6684 AD - Department of Orthopaedic Surgery, University Hospital Magdeburg, Otto von Guericke University Magdeburg, Leipziger Strasse 44, 39120 Magdeburg, Germany. FAU - Ferl, Mark AU - Ferl M AD - Department of Orthopaedic Surgery, University Hospital Magdeburg, Otto von Guericke University Magdeburg, Leipziger Strasse 44, 39120 Magdeburg, Germany. FAU - Lohmann, Christoph H AU - Lohmann CH AUID- ORCID: 0000-0002-2962-2183 AD - Department of Orthopaedic Surgery, University Hospital Magdeburg, Otto von Guericke University Magdeburg, Leipziger Strasse 44, 39120 Magdeburg, Germany. FAU - Schonrogge, Maria AU - Schonrogge M AUID- ORCID: 0000-0001-8904-3637 AD - Department of Orthopaedic Surgery, University Hospital Magdeburg, Otto von Guericke University Magdeburg, Leipziger Strasse 44, 39120 Magdeburg, Germany. LA - eng PT - Clinical Trial Protocol PT - Journal Article DEP - 20261020 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Prospective Studies MH - *Risk Assessment/methods/standards MH - Observational Studies as Topic MH - Validation Studies as Topic MH - *Surveys and Questionnaires/standards MH - *Spine/surgery MH - *Spinal Diseases/psychology/surgery MH - Humans MH - Perioperative Care/instrumentation/methods/standards MH - Reproducibility of Results MH - Disability Evaluation PMC - PMC13707344 OTO - NOTNLM OT - Type-D personality OT - biopsychosocial model OT - clinical prediction model OT - digital health OT - patient-reported outcome measures OT - risk stratification OT - spine surgery OT - study protocol COIS- The authors declare no conflicts of interest. The SpinePROTECT software was developed as an institutional academic project without commercial sponsorship. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/20 CRDT- 2026/10/27 10:42 PHST- 2026/09/05 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - medicina62102027 [pii] AID - medicina-62-02027 [pii] AID - 10.3390/medicina62102027 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 20;62(10):2027. doi: 10.3390/medicina62102027. PMID- 42942557 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 20 TI - FGFR 1-3 Alteration Frequency in Muscle-Invasive Urothelial Bladder Cancer and Its Effect on Prognosis. LID - 10.3390/medicina62102026 [doi] LID - 2026 AB - Background and Objectives: Fibroblast growth factor receptor (FGFR) alterations are therapeutic targets in urothelial carcinoma, but their prognostic significance in muscle-invasive bladder cancer (MIBC) is uncertain. We evaluated the frequency of FGFR1-3 alterations and their association with overall survival (OS) in Turkish patients with MIBC. Materials and Methods: Formalin-fixed, paraffin-embedded tumour samples from 194 patients with non-metastatic muscle-invasive (n = 154) or metastatic (n = 40) urothelial carcinoma of the bladder were analysed by targeted next-generation sequencing of FGFR1-3. Variants were classified according to ACMG/AMP tiers. OS was assessed with Kaplan-Meier and Cox regression analyses in the overall cohort and in both subgroups. Results: At least one FGFR alteration was detected in 131 patients (67.5%): FGFR3 in 42.8%, FGFR1 in 24.7% and FGFR2 in 20.6%. Most FGFR1 (91.7%) and FGFR2 (87.5%) alterations were Tier III variants, whereas Tier I FGFR3 hotspot mutations were found in 17.5% of the cohort. Median OS was shorter in FGFR-altered than in FGFR-unaltered patients (14 vs. 18 months; p = 0.024), with a similar difference in the non-metastatic subgroup (19 vs. 24 months; p = 0.047) but not in the metastatic subgroup (8 vs. 7 months; p = 0.653). After adjustment for metastatic status, ECOG performance status and age, overall FGFR alteration status was not an independent prognostic factor (HR 1.31, 95% CI 0.87-1.97; p = 0.200), whereas FGFR1 alteration was independently associated with worse OS (HR 1.62, 95% CI 1.07-2.44; p = 0.023). FGFR3 alterations were not prognostic. Conclusions: FGFR alterations, predominantly in FGFR3, are frequent in MIBC and are associated with shorter OS, but not independently of metastatic status and performance status. FGFR1 alteration was associated with poor prognosis, but this finding requires validation in larger cohorts. FAU - Caglayan, Dilek AU - Caglayan D AUID- ORCID: 0000-0003-4629-6815 AD - Department of Medical Oncology, Faculty of Medicine, Necmettin Erbakan University, Konya,42090, Turkey. FAU - Goktas, Emine AU - Goktas E AD - Department of Medical Genetics, Faculty of Medicine, Necmettin Erbakan University, Konya 42090, Turkey. FAU - Somuncu, Makbule Nihan AU - Somuncu MN AUID- ORCID: 0000-0001-6041-457X AD - Department of Medical Genetics, Faculty of Medicine, Necmettin Erbakan University, Konya 42090, Turkey. FAU - Kilinc, Fahriye AU - Kilinc F AUID- ORCID: 0000-0002-1202-8735 AD - Department of Pathology, Faculty of Medicine, Necmettin Erbakan University, Konya 42090, Turkey. FAU - Kocak, Mehmet Zahid AU - Kocak MZ AUID- ORCID: 0000-0003-3085-7964 AD - Department of Medical Oncology, Faculty of Medicine, Necmettin Erbakan University, Konya,42090, Turkey. FAU - Hendem, Engin AU - Hendem E AUID- ORCID: 0000-0001-6970-4733 AD - Department of Medical Oncology, Mengucek Gazi Training and Research Hospital, Erzincan 24100, Turkey. FAU - Yildiz, Oguzhan AU - Yildiz O AUID- ORCID: 0000-0002-4057-3108 AD - Department of Medical Oncology, Faculty of Medicine, Necmettin Erbakan University, Konya,42090, Turkey. FAU - Gurbuz, Ali Fuat AU - Gurbuz AF AUID- ORCID: 0000-0003-1455-471X AD - Department of Medical Oncology, Faculty of Medicine, Necmettin Erbakan University, Konya,42090, Turkey. FAU - Canlar, Hilal AU - Canlar H AD - Department of Internal Medicine, Faculty of Medicine, Necmettin Erbakan University, Konya 42090, Turkey. FAU - Ugrakli, Muzaffer AU - Ugrakli M AUID- ORCID: 0000-0003-0544-6047 AD - Department of Medical Oncology, Faculty of Medicine, Necmettin Erbakan University, Konya,42090, Turkey. FAU - Eryilmaz, Melek Karakurt AU - Eryilmaz MK AUID- ORCID: 0000-0003-2597-5931 AD - Department of Medical Oncology, Faculty of Medicine, Necmettin Erbakan University, Konya,42090, Turkey. FAU - Artac, Mehmet AU - Artac M AUID- ORCID: 0000-0003-2335-3354 AD - Department of Medical Oncology, Faculty of Medicine, Necmettin Erbakan University, Konya,42090, Turkey. FAU - Araz, Murat AU - Araz M AUID- ORCID: 0000-0002-4632-9501 AD - Department of Medical Oncology, Faculty of Medicine, Necmettin Erbakan University, Konya,42090, Turkey. LA - eng GR - 23DA18003/Necmettin Erbakan University/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 3) RN - EC 2.7.10.1 (FGFR3 protein, human) RN - EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 1) RN - EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 2) RN - EC 2.7.10.1 (FGFR1 protein, human) RN - EC 2.7.10.1 (FGFR2 protein, human) SB - IM MH - Humans MH - Female MH - Male MH - *Urinary Bladder Neoplasms/genetics/mortality MH - Prognosis MH - *Receptor, Fibroblast Growth Factor, Type 3/genetics/analysis MH - Aged MH - Middle Aged MH - *Receptor, Fibroblast Growth Factor, Type 1/genetics/analysis MH - Turkey MH - Aged, 80 and over MH - Receptor, Fibroblast Growth Factor, Type 2/genetics/analysis MH - Mutation MH - Cohort Studies MH - Kaplan-Meier Estimate MH - Proportional Hazards Models PMC - PMC13706912 OTO - NOTNLM OT - FGFR alterations OT - muscle-invasive bladder cancer OT - prognosis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/20 CRDT- 2026/10/27 10:42 PHST- 2026/09/10 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - medicina62102026 [pii] AID - medicina-62-02026 [pii] AID - 10.3390/medicina62102026 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 20;62(10):2026. doi: 10.3390/medicina62102026. PMID- 42942553 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 19 TI - Optimizing Indocyanine Green Fluorescence Cholangiography in Laparoscopic Cholecystectomy: A Systematic Review of Randomized Controlled Trials on Dose, Timing, Route, Efficacy, and Safety. LID - 10.3390/medicina62102022 [doi] LID - 2022 AB - Background and Objectives: Near-infrared fluorescence cholangiography with indocyanine green (ICG) has been proposed as an adjunct for improving biliary anatomy recognition during laparoscopic cholecystectomy. However, uncertainty remains regarding its comparative efficacy, operative value, safety, and optimal route, dose, and timing. Materials and Methods: A systematic review was conducted to identify randomized comparative and dose-finding studies evaluating ICG fluorescence cholangiography during laparoscopic cholecystectomy. Outcomes included visualization of the cystic duct, common hepatic duct, common bile duct, biliary junctions, operative time, time to critical anatomical identification, conversion or bailout procedures, length of stay, bile duct injury, bile leak, and ICG-related adverse events. Clinical and methodological heterogeneity precluded meta-analysis; findings were synthesized descriptively. Results: Eighteen studies were included. ICG improved visualization of selected biliary structures compared with white-light imaging, particularly before dissection, whereas differences versus radiographic intraoperative cholangiography were less pronounced. Operative effects were inconsistent: several studies reported shorter cholangiography duration or earlier anatomical identification, without consistent reductions in total operative time or length of stay. Bile duct injuries and bile leaks were infrequent, but event numbers were insufficient to establish a protective effect or comparative safety. Selected dose comparisons suggested reduced hepatic background fluorescence with lower doses, although findings depended on administration timing and imaging platform. Interpretation was limited by subjective visualization assessment, risk of bias, and heterogeneous patient populations. Conclusions: ICG fluorescence cholangiography improves visualization of selected biliary structures, but improved visualization should not be equated with demonstrated clinical benefit. Evidence remains insufficient to establish reductions in bile duct injury, conversion, or postoperative morbidity, or a universally optimal route, dose, or timing regimen. FAU - Tsavdaris, Dimitrios AU - Tsavdaris D AD - First Department of Propaedeutic Surgery, University General Hospital of Thessaloniki AHEPA, Aristotle University of Thessaloniki, 54636 Thessaloniki, Greece. FAU - Geropoulos, Georgios AU - Geropoulos G AD - Third Department of Surgery, University General Hospital of Thessaloniki AHEPA, Aristotle University of Thessaloniki, 54636 Thessaloniki, Greece. FAU - Gialouris, Anastasios AU - Gialouris A AD - First Department of Propaedeutic Surgery, University General Hospital of Thessaloniki AHEPA, Aristotle University of Thessaloniki, 54636 Thessaloniki, Greece. FAU - Mekras, Alexandros AU - Mekras A AUID- ORCID: 0000-0001-6839-370X AD - Department of General and Visceral Surgery, SHG-Klinikum Merzig, Academic Hospital of University of Saarland, 66663 Merzig, Germany. FAU - Psarras, Kyriakos AU - Psarras K AUID- ORCID: 0000-0001-9507-9978 AD - Third Department of Surgery, University General Hospital of Thessaloniki AHEPA, Aristotle University of Thessaloniki, 54636 Thessaloniki, Greece. FAU - Paramythiotis, Daniel AU - Paramythiotis D AUID- ORCID: 0000-0001-9787-7391 AD - First Department of Propaedeutic Surgery, University General Hospital of Thessaloniki AHEPA, Aristotle University of Thessaloniki, 54636 Thessaloniki, Greece. LA - eng PT - Journal Article PT - Review PT - Systematic Review DEP - 20261019 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - IX6J1063HV (Indocyanine Green) SB - IM MH - Humans MH - *Indocyanine Green/administration & dosage/therapeutic use MH - *Cholecystectomy, Laparoscopic/methods/standards MH - *Cholangiography/methods/standards MH - Randomized Controlled Trials as Topic MH - Fluorescence PMC - PMC13707089 OTO - NOTNLM OT - biliary anatomy OT - fluorescence cholangiography OT - indocyanine green OT - laparoscopic cholecystectomy OT - near-infrared imaging OT - systematic review COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/19 CRDT- 2026/10/27 10:42 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - medicina62102022 [pii] AID - medicina-62-02022 [pii] AID - 10.3390/medicina62102022 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 19;62(10):2022. doi: 10.3390/medicina62102022. PMID- 42942552 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 19 TI - Early Dynamic (18)F-FDG PET/CT SUVmax and Its Association with Pathological Stage and Tumor Grade in Bladder Cancer: A Prospective Exploratory Study. LID - 10.3390/medicina62102021 [doi] LID - 2021 AB - Background and Objectives: Accurate preoperative staging and grade assessment are crucial for optimal treatment selection in bladder cancer. This prospective study evaluated multi-timepoint Fluorine-18 fluorodeoxyglucose ((18)F-FDG) positron emission tomography/computed tomography (PET/CT) imaging for predicting pathological stage and tumor grade. Materials and Methods: Seventy-two patients with bladder carcinoma underwent (18)F-FDG PET/CT including early dynamic (0-2, 2-4, 8-10 min), standard (60 min), and late (~120 min) imaging following a standardized furosemide hydration protocol. Maximum standardized uptake values (SUVmax) were correlated with histopathological findings. Diagnostic performance was evaluated using receiver operating characteristic (ROC) curve analysis with Youden index for optimal cut-offs. Results: The cohort comprised 72 patients (84.7% male, mean age 64.8 +/- 10.7 years) with pathological stages Ta (36.1%), CIS (15.3%), T1 (31.9%), and T2 (16.7%). High-grade tumors demonstrated significantly higher SUVmax at early dynamic imaging (0-2 min: 1.50 vs. 1.00, p = 0.028) and late imaging (11.40 vs. 9.50, p = 0.045) compared to low-grade tumors. Early dynamic imaging at 0-2 min achieved the highest, although modest, diagnostic performance for high-grade prediction (area under the curve (AUC) = 0.652) and muscle-invasive disease prediction (AUC = 0.669), with optimal cut-offs of SUVmax = 1.40 and 1.80, respectively. Early dynamic SUVmax correlated significantly with tumor grade (rho = 0.261, p = 0.027) and tumor size (rho = 0.424, p < 0.001). The negative predictive value for muscle-invasive disease was 90.4%, but sensitivity was only 58.3%. Conclusions: Early dynamic (18)F-FDG PET/CT imaging at 0-2 min showed the highest, although modest, diagnostic performance among the evaluated time points for predicting tumor grade and muscle invasion. The identified SUVmax cut-offs are exploratory and require external validation in larger multicenter studies before clinical use. Trial registration: Not registered. FAU - Saylam, Baris AU - Saylam B AUID- ORCID: 0000-0003-3256-8752 AD - Department of Urology, Mersin City Training and Research Hospital, 33240 Mersin, Turkey. FAU - Temelli, Berfin AU - Temelli B AUID- ORCID: 0009-0009-7899-1637 AD - Department of Nuclear Medicine, Mersin City Training and Research Hospital, 33240 Mersin, Turkey. FAU - Nebioglu, Ali AU - Nebioglu A AUID- ORCID: 0000-0001-6325-1534 AD - Department of Urology, Mersin City Training and Research Hospital, 33240 Mersin, Turkey. FAU - Efesoy, Ozan AU - Efesoy O AD - Department of Urology, Mersin City Training and Research Hospital, 33240 Mersin, Turkey. FAU - Bozlu, Murat AU - Bozlu M AUID- ORCID: 0000-0002-8624-0149 AD - Department of Urology, Mersin University Faculty of Medicine, 33343 Mersin, Turkey. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - 0Z5B2CJX4D (Fluorodeoxyglucose F18) RN - 0 (Radiopharmaceuticals) SB - IM MH - Humans MH - Male MH - Female MH - Prospective Studies MH - *Fluorodeoxyglucose F18/therapeutic use MH - *Positron Emission Tomography Computed Tomography/methods/standards MH - *Urinary Bladder Neoplasms/pathology/diagnostic imaging MH - Middle Aged MH - Aged MH - Neoplasm Staging/methods MH - Neoplasm Grading/methods MH - ROC Curve MH - Radiopharmaceuticals PMC - PMC13706885 OTO - NOTNLM OT - 18F-FDG PET/CT OT - bladder cancer OT - early dynamic imaging OT - non-invasive staging OT - standardized uptake value COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/19 CRDT- 2026/10/27 10:42 PHST- 2026/08/25 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - medicina62102021 [pii] AID - medicina-62-02021 [pii] AID - 10.3390/medicina62102021 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 19;62(10):2021. doi: 10.3390/medicina62102021. PMID- 42942541 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 17 TI - Mild Autonomous Cortisol Secretion Is Associated with Computed Tomography-Derived Hepatic Steatosis in Patients with Adrenal Incidentaloma. LID - 10.3390/medicina62102010 [doi] LID - 2010 AB - Background and Objectives: Mild autonomous cortisol secretion (MACS) has been associated with adverse metabolic outcomes, but its relationship with hepatic steatosis remains incompletely understood. This study aimed to compare computed tomography (CT)-derived hepatic steatosis parameters between patients with MACS and non-functioning adrenal incidentaloma (NFAI) and to evaluate the associations between biochemical markers of cortisol secretion and hepatic attenuation measures. Materials and Methods: This retrospective study comprised 123 patients with adrenal incidentaloma (39 MACS and 84 NFAI) who underwent unenhanced abdominal CT between January 2020 and November 2024. Hepatic steatosis was assessed using mean liver attenuation, liver-spleen attenuation difference, liver-to-spleen attenuation ratio, and conventional CT-defined steatosis thresholds. Non-invasive metabolic indices were also computed. Associations between cortisol secretion parameters and CT-derived hepatic attenuation measures were examined using correlation analysis and multivariable regression. Results: The liver-to-spleen attenuation ratio was lower in the MACS group, although this difference did not reach statistical significance (p = 0.050), and a significantly lower liver-spleen attenuation difference (p = 0.048) was observed compared with the NFAI group, whereas mean liver attenuation (p = 0.167), conventional CT-defined hepatic steatosis, and non-invasive metabolic indices did not differ significantly between groups. Among biochemical markers of cortisol secretion, only 24-h urinary free cortisol showed significant correlations with all CT-derived hepatic attenuation parameters. After adjustment for age, sex, and BMI, urinary free cortisol remained significantly associated with liver-spleen attenuation difference (p = 0.047), whereas its associations with liver attenuation (p = 0.056) and liver-to-spleen attenuation ratio (p = 0.051) did not reach statistical significance. Conclusions: Patients with MACS exhibit subtle CT-derived hepatic fat accumulation despite similar conventional CT-defined steatosis and non-invasive metabolic indices compared with patients with NFAI. Continuous CT-derived attenuation parameters may be more sensitive than conventional CT-defined steatosis thresholds for detecting subtle hepatic fat accumulation associated with mild cortisol excess. The consistent associations observed between 24-h urinary free cortisol and CT-derived hepatic attenuation parameters, including the significant association with liver-spleen attenuation difference after adjustment for age, sex, and BMI, warrant confirmation in larger prospective studies. FAU - Alkan, Samet AU - Alkan S AUID- ORCID: 0000-0003-3172-2770 AD - Department of Endocrinology and Metabolic Diseases, Faculty of Medicine, Manisa Celal Bayar University, 45030 Manisa, Turkiye. AD - Department of Endocrinology and Metabolic Diseases, Izmir City Hospital, 35540 Izmir, Turkiye. FAU - Farasat, Mustafa AU - Farasat M AUID- ORCID: 0000-0002-8019-1235 AD - Department of Radiology, Faculty of Medicine, Manisa Celal Bayar University, 45030 Manisa, Turkiye. FAU - Akcura, Can AU - Akcura C AUID- ORCID: 0000-0003-4182-9002 AD - Department of Endocrinology and Metabolic Diseases, Faculty of Medicine, Manisa Celal Bayar University, 45030 Manisa, Turkiye. AD - Department of Endocrinology and Metabolic Diseases, Democracy University Buca Seyfi Demirsoy Education and Research Hospital, 35390 Izmir, Turkiye. FAU - Guney, Sedat Can AU - Guney SC AUID- ORCID: 0000-0002-2035-6843 AD - Department of Endocrinology and Metabolic Diseases, Faculty of Medicine, Manisa Celal Bayar University, 45030 Manisa, Turkiye. AD - Department of Endocrinology and Metabolic Diseases, Bakircay University Cigli Education and Research Hospital, 35620 Izmir, Turkiye. FAU - Buyuker, Merve AU - Buyuker M AD - Department of Radiology, Faculty of Medicine, Manisa Celal Bayar University, 45030 Manisa, Turkiye. AD - Department of Radiology, Sarikamis State Hospital, 36200 Kars, Turkiye. FAU - Pekindil, Gokhan AU - Pekindil G AD - Department of Radiology, Faculty of Medicine, Manisa Celal Bayar University, 45030 Manisa, Turkiye. FAU - Ozdemir, Nilufer AU - Ozdemir N AUID- ORCID: 0000-0002-0719-988X AD - Department of Endocrinology and Metabolic Diseases, Faculty of Medicine, Manisa Celal Bayar University, 45030 Manisa, Turkiye. FAU - Hekimsoy, Zeliha AU - Hekimsoy Z AD - Department of Endocrinology and Metabolic Diseases, Faculty of Medicine, Manisa Celal Bayar University, 45030 Manisa, Turkiye. LA - eng PT - Journal Article DEP - 20261017 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - WI4X0X7BPJ (Hydrocortisone) RN - Adrenal incidentaloma SB - IM MH - Humans MH - Female MH - *Fatty Liver/diagnostic imaging MH - *Hydrocortisone/blood/analysis/metabolism MH - Retrospective Studies MH - Male MH - *Adrenal Gland Neoplasms/complications MH - Middle Aged MH - *Tomography, X-Ray Computed/methods MH - Aged MH - Liver/diagnostic imaging PMC - PMC13706875 OTO - NOTNLM OT - MASLD OT - adrenal incidentaloma OT - computed tomography OT - hepatic steatosis OT - liver attenuation OT - mild autonomous cortisol secretion OT - urinary free cortisol COIS- The authors have no conflicts of interest to declare. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/17 CRDT- 2026/10/27 10:42 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - medicina62102010 [pii] AID - medicina-62-02010 [pii] AID - 10.3390/medicina62102010 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 17;62(10):2010. doi: 10.3390/medicina62102010. PMID- 42942536 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 16 TI - The Role of Hysteroscopy in Reproductive Medicine and Subfertility: A Critical Narrative Review. LID - 10.3390/medicina62102005 [doi] LID - 2005 AB - Background and Objectives: Hysteroscopy permits direct assessment and treatment of the uterine cavity, but diagnostic yield does not necessarily translate into improved fertility. This narrative review examines its role in subfertility and reproductive medicine. Materials and Methods: The original literature search covered PubMed/MEDLINE, Scopus and the Cochrane Library through February 2026. Targeted checks during revision added the 2026 chronic endometritis consensus and the full CERM trial report; these updates do not represent a new comprehensive search. Evidence was interpreted by study design, population, outcome and precision, without formal risk-of-bias assessment or meta-analysis. Results: Large randomised trials do not support routine hysteroscopy before first IVF or after repeated IVF failure when ultrasonography is normal. Polypectomy has evidence of improved pregnancy rates in women planning IUI, although spontaneous pregnancies contributed to that result and live-birth benefit remains uncertain. Hysteroscopic myomectomy may improve clinical pregnancy in women with cavity-distorting fibroids; evidence for live birth is less secure. Adhesiolysis restores cavity anatomy, but reproductive estimates largely derive from observational series. The small TRUST trial did not demonstrate a live-birth advantage from septum resection and cannot establish equivalence. Hysteroscopy may guide biopsy for chronic endometritis but does not replace histological assessment. CERM found no improvement with doxycycline in its composite of cumulative live births and ongoing pregnancies at >/=24 weeks among women with recurrent miscarriage and CD138-defined endometritis. Fertility-sparing endometrial cancer treatment requires oncological selection, progestin therapy and histological surveillance. Conclusions: Hysteroscopy should address a defined diagnostic or therapeutic question. Decisions should distinguish spontaneous conception, IUI and IVF/ICSI and acknowledge uncertainty in live-birth outcomes. FAU - Tsonis, Orestis AU - Tsonis O AUID- ORCID: 0000-0002-0404-994X AD - Fertility Preservation Service, Assisted Conception Unit, Guy's and St Thomas' NHS Foundation Trust, London SE1 9RT, UK. AD - Department of Obstetrics and Gynaecology, University Hospital of Ioannina, 455 00 Ioannina, Greece. FAU - Paschopoulos, Ioannis AU - Paschopoulos I AUID- ORCID: 0009-0000-6221-7537 AD - School of Medicine, Faculty of Health Sciences, National and Kapodistrian University of Athens, 115 27 Athens, Greece. FAU - Paschopoulou, Kasmiria Ioanna AU - Paschopoulou KI AD - School of Medicine, Faculty of Health Sciences, University of Ioannina, 451 10 Ioannina, Greece. FAU - Deligeoroglou, Evangelia AU - Deligeoroglou E AUID- ORCID: 0009-0005-2772-3684 AD - Unit for Human Reproduction, Medical School, Aristotle University of Thessaloniki, 1st Department of Obstetrics & Gynecology, Papageorgiou General Hospital, 564 03 Thessaloniki, Greece. FAU - Barmpalia, Zoi AU - Barmpalia Z AUID- ORCID: 0000-0002-5516-7786 AD - Department of Obstetrics and Gynaecology, University Hospital of Ioannina, 455 00 Ioannina, Greece. FAU - Paschopoulos, Minas AU - Paschopoulos M AD - Department of Obstetrics and Gynaecology, University Hospital of Ioannina, 455 00 Ioannina, Greece. LA - eng PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - Female MH - *Hysteroscopy/methods/standards MH - Pregnancy MH - *Reproductive Medicine/methods MH - *Infertility, Female MH - Endometritis PMC - PMC13706842 OTO - NOTNLM OT - assisted reproduction OT - chronic endometritis OT - endometrial polyp OT - hysteroscopy OT - intrauterine adhesions OT - septate uterus OT - subfertility OT - submucous fibroid COIS- The authors declare no conflicts of interest. O.T. participated as an expert panellist in the international e-Delphi consensus study on chronic endometritis cited in this review. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/16 CRDT- 2026/10/27 10:42 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - medicina62102005 [pii] AID - medicina-62-02005 [pii] AID - 10.3390/medicina62102005 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 16;62(10):2005. doi: 10.3390/medicina62102005. PMID- 42942533 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 16 TI - HDL-C Levels Are Associated with Clinical Outcomes and Hospital Mortality in Critically Ill Patients with Sepsis: A Prospective Cohort Study. LID - 10.3390/medicina62102002 [doi] LID - 2002 AB - Background and Objectives: Metabolic perturbations frequently occur during the acute phase of critical illness and may disrupt lipid homeostasis. Acute respiratory distress syndrome (ARDS) is a severe condition and is associated with high mortality in critically ill patients with sepsis. This study aimed to evaluate the association between conventional plasma lipid parameters and clinical outcomes in critically ill sepsis patients. Materials and Methods: We conducted a prospective observational cohort study of critically ill sepsis patients in Taiwan between October 2020 and July 2025. Conventional plasma lipid parameters and clinical variables were measured at intensive care unit admission. Clinical outcomes were compared between patients stratified by plasma HDL-C levels. Results: A total of 285 critically ill patients with sepsis were included. Pneumonia was the presumed source of infection in 91% of patients, and 78% met the criteria for ARDS. The overall hospital mortality rate was 37.9%. Patients with ARDS had significantly higher C-reactive protein (CRP) and interleukin-6 (IL-6) levels, higher risk of organ failure (i.e., APACHE II and SOFA scores), and lower plasma HDL-C levels (all p < 0.05). Non-survivors exhibited significantly greater organ failure severity and lower HDL-C levels compared with survivors (all p < 0.05). Patients with low HDL-C ( 17.5 mg/dL; n = 179, 62.8%) (all p < 0.05). In multivariable analyses, lower HDL-C were independently associated with hospital mortality. Conclusions: Lower HDL-C levels were associated with hospital mortality in critically ill patients with sepsis, most of whom had pneumonia and concomitant ARDS. These findings suggest that HDL-C may have prognostic value in this selected critically ill population, although external validation in broader sepsis populations is warranted. An easily accessible and useful for early risk stratification. FAU - Li, Hsin-Hsien AU - Li HH AUID- ORCID: 0000-0002-3888-4820 AD - Department of Respiratory Therapy, Chang Gung University College of Medicine, Taoyuan 33302, Taiwan. AD - Department of Thoracic Medicine, Chang Gung Memorial Hospital, Linkou Branch, Taoyuan 33305, Taiwan. FAU - Chan, Tien-Ming AU - Chan TM AD - Division of Rheumatology, Allergy, and Immunology, Chang Gung Memorial Hospital, Linkou Branch, Taoyuan 33305, Taiwan. AD - College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan. FAU - Ko, How-Wen AU - Ko HW AD - Department of Thoracic Medicine, Chang Gung Memorial Hospital, Linkou Branch, Taoyuan 33305, Taiwan. AD - College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan. FAU - Lee, Chung-Shu AU - Lee CS AUID- ORCID: 0000-0003-0466-7796 AD - Department of Thoracic Medicine, Chang Gung Memorial Hospital, Linkou Branch, Taoyuan 33305, Taiwan. AD - College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan. AD - Department of Thoracic Medicine, New Taipei Municipal TuCheng Hospital and Chang Gung University, Taoyuan 33302, Taiwan. FAU - Hsu, Ping-Chih AU - Hsu PC AD - Department of Thoracic Medicine, Chang Gung Memorial Hospital, Linkou Branch, Taoyuan 33305, Taiwan. AD - College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan. FAU - Kuo, Scott Chih-Hsi AU - Kuo SC AD - Department of Thoracic Medicine, Chang Gung Memorial Hospital, Linkou Branch, Taoyuan 33305, Taiwan. AD - College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan. FAU - Hu, Han-Chung AU - Hu HC AUID- ORCID: 0000-0003-1603-868X AD - Department of Thoracic Medicine, Chang Gung Memorial Hospital, Linkou Branch, Taoyuan 33305, Taiwan. AD - College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan. FAU - Chiu, Li-Chung AU - Chiu LC AUID- ORCID: 0000-0003-1660-7715 AD - Department of Thoracic Medicine, Chang Gung Memorial Hospital, Linkou Branch, Taoyuan 33305, Taiwan. AD - College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan. LA - eng GR - CMRPG3L0821/Chang Gung Memorial Hospital/ GR - CMRPG3L0822/Chang Gung Memorial Hospital/ GR - CORPG3M0331/Chang Gung Memorial Hospital/ GR - CMRPG3N1121/Chang Gung Memorial Hospital/ GR - MOST 111-2314-B-182A-148/Ministry of Science and Technology/ GR - NSTC114-2314-B-182-065-/National Science and Technology Council/ PT - Journal Article PT - Observational Study DEP - 20261016 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - 0 (Biomarkers) RN - 9007-41-4 (C-Reactive Protein) RN - 0 (Cholesterol, HDL) RN - 0 (Interleukin-6) SB - IM MH - Aged MH - Female MH - Humans MH - Male MH - Middle Aged MH - APACHE MH - Biomarkers/blood MH - C-Reactive Protein/analysis MH - *Cholesterol, HDL/blood MH - Cohort Studies MH - *Critical Illness/mortality MH - *Hospital Mortality MH - Intensive Care Units MH - Interleukin-6/blood MH - Prospective Studies MH - Respiratory Distress Syndrome/blood/mortality MH - *Sepsis/blood/diagnosis/mortality MH - Taiwan/epidemiology PMC - PMC13707096 OTO - NOTNLM OT - acute respiratory distress syndrome OT - conventional lipid parameters OT - critical illness OT - high-density lipoprotein cholesterol OT - mortality OT - outcomes OT - sepsis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/16 CRDT- 2026/10/27 10:42 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - medicina62102002 [pii] AID - medicina-62-02002 [pii] AID - 10.3390/medicina62102002 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 16;62(10):2002. doi: 10.3390/medicina62102002. PMID- 42942531 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 15 TI - Association of FOXP3 Gene Polymorphisms with Skin, Vulvar and Ectocervical Squamous Cell Tumors. LID - 10.3390/medicina62102000 [doi] LID - 2000 AB - Background and Objectives: Squamous cell tumors arise from squamous epithelial cells. Polymorphisms in the FOXP3 gene may influence malignancy susceptibility. This study evaluated the potential association of FOXP3 rs3761548 (-3279 A/C) and rs2280883 (IVS9+459 T/C) polymorphisms with preinvasive and invasive squamous cell tumors of sun-exposed skin, sun-protected vulvar skin, and the ectocervix. Materials and Methods: This comparative observational study included 135 women aged >18 years surgically treated for squamous cell tumors of the skin (actinic keratosis, Bowen's disease, squamous cell carcinoma), vulva (HSIL, differentiated VIN, squamous cell carcinoma), or ectocervix (LSIL, HSIL, squamous cell carcinoma). Sociodemographic, anthropometric, lifestyle, ultraviolet radiation exposure, reproductive, and sexual health data were collected. The FOXP3 rs3761548 and rs2280883 polymorphisms were genotyped; codominant, dominant, recessive, and overdominant genetic models were evaluated. Results: The three groups differed significantly in several sociodemographic, anthropometric, lifestyle, reproductive, and sexual health characteristics. No significant differences were observed for smoking, sun exposure, history of sunburns, contraceptive use, or history of abortions. The minor A allele frequency of rs3761548 was lower in the skin and vulvar tumor groups and slightly higher in the ectocervical tumor group than in reference populations; however, this difference was not significant (p = 0.066). None of the rs3761548 genetic models remained significant after LASSO regression. For rs2280883, the C allele frequency was lower in the skin and ectocervical groups but higher in the vulvar tumor group than the 46.0% reported in the reference population. The dominant model (CC + CT vs. TT) was associated with lower odds of skin tumors, whereas the recessive model (CC vs. TT + CT) was associated with higher odds (both p = 0.037). Conclusions: The distribution of the FOXP3 rs2280883 polymorphism differed according to tumor location. It was significantly associated with cutaneous squamous cell tumors under both dominant and recessive genetic models. FAU - Kotlica, Boba AU - Kotlica B AUID- ORCID: 0009-0000-1556-2696 AD - Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia. FAU - Stefanovic, Katarina AU - Stefanovic K AUID- ORCID: 0000-0001-5005-0207 AD - Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia. AD - Clinic for Gynecology and Obstetrics, University Clinical Center of Serbia, 11000 Belgrade, Serbia. FAU - Argenziano, Giuseppe AU - Argenziano G AUID- ORCID: 0000-0003-1413-8214 AD - Dermatology Unit, University of Campania "Luigi Vanvitelli", 81100 Naples, Italy. FAU - Stojicic, Milan AU - Stojicic M AUID- ORCID: 0000-0002-4545-4335 AD - Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia. AD - Clinic for Burns, Plastic and Reconstructive Surgery, University Clinical Center of Serbia, 11000 Belgrade, Serbia. FAU - Stojkovic, Goran AU - Stojkovic G AUID- ORCID: 0000-0003-3659-6136 AD - Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia. AD - Clinic for Otorhinolaryngology and Maxillofacial Surgery, University Clinical Center Serbia, 11000 Belgrade, Serbia. FAU - Pantovic, Sveto AU - Pantovic S AD - Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia. AD - Clinic for Gynecology and Obstetrics, University Clinical Center of Serbia, 11000 Belgrade, Serbia. FAU - Tulic, Lidija AU - Tulic L AUID- ORCID: 0000-0001-5706-0707 AD - Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia. AD - Clinic for Gynecology and Obstetrics, University Clinical Center of Serbia, 11000 Belgrade, Serbia. FAU - Cirkovic, Anda AU - Cirkovic A AUID- ORCID: 0000-0002-7542-5690 AD - Institute for Medical Statistics and Informatics, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia. FAU - Milenkovic, Andela Dimkic AU - Milenkovic AD AD - Clinic for Burns, Plastic and Reconstructive Surgery, University Clinical Center of Serbia, 11000 Belgrade, Serbia. FAU - Todorovic, Nikola AU - Todorovic N AD - Clinic for Otorhinolaryngology and Maxillofacial Surgery, University Clinical Center Serbia, 11000 Belgrade, Serbia. FAU - Novakovic, Ivana AU - Novakovic I AD - Institute of Human Genetics, Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia. LA - eng PT - Comparative Study PT - Journal Article PT - Observational Study DEP - 20261015 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - 0 (Forkhead Transcription Factors) RN - 0 (FOXP3 protein, human) SB - IM MH - Adult MH - Aged MH - Female MH - Humans MH - Middle Aged MH - *Carcinoma, Squamous Cell/genetics MH - *Forkhead Transcription Factors/genetics/analysis/blood MH - Genetic Predisposition to Disease MH - *Skin Neoplasms/genetics MH - *Vulvar Neoplasms/genetics PMC - PMC13707306 OTO - NOTNLM OT - Bowen's disease OT - FOXP3 OT - HSIL OT - LSIL OT - SCC OT - VIN OT - actinic keratosis OT - rs2280883 OT - rs3761548 COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/15 CRDT- 2026/10/27 10:42 PHST- 2026/09/07 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - medicina62102000 [pii] AID - medicina-62-02000 [pii] AID - 10.3390/medicina62102000 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 15;62(10):2000. doi: 10.3390/medicina62102000. PMID- 42942528 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 15 TI - Association of Preoperative Lactate and LDH Levels with Resection Length in Acute Mesenteric Ischemia. LID - 10.3390/medicina62101997 [doi] LID - 1997 AB - Background and Objectives: This study examined the associations of admission lactate and lactate dehydrogenase (LDH) levels with total small bowel resection length in patients with acute mesenteric ischemia (AMI) who underwent resection. Materials and Methods: This retrospective, single-center study included 71 patients who underwent small bowel resection for AMI. Associations were assessed using Spearman's correlation and linear regression adjusted for albumin and AMI etiology. Heteroscedasticity-consistent HC3 standard errors were used for regression inference. Model diagnostics and sensitivity analyses were performed. Admission biomarker levels were also compared by in-hospital mortality status in an exploratory analysis. Results: The median resection length was 90 cm (range, 20-360 cm) and this correlated with lactate (rho = 0.551, p < 0.001) and LDH (rho = 0.317, p = 0.007). In the etiology-adjusted model, lactate (B = 12.286, HC3 95% CI: 4.449-20.123; p = 0.003) and LDH (B = 0.035, HC3 95% CI: 0.014-0.056; p = 0.002) remained associated with resection length. The apparent R(2) was 0.567, and both associations remained positive after influential observations were excluded. In exploratory unadjusted comparisons, non-survivors had higher admission lactate and LDH levels than survivors. Conclusions: Admission lactate and LDH levels were associated with resection length in this selected surgical cohort. Individual-level predictive accuracy was not established, and these biomarkers cannot currently guide operative decisions. FAU - Hidiroglu, Mehmet Mert AU - Hidiroglu MM AUID- ORCID: 0009-0009-1137-8803 AD - Department of General Surgery, Gulhane Training and Research Hospital, University of Health Sciences, Ankara 06010, Turkiye. FAU - Yavuz, Ahmet AU - Yavuz A AUID- ORCID: 0009-0008-1039-1478 AD - Department of General Surgery, Gulhane Training and Research Hospital, University of Health Sciences, Ankara 06010, Turkiye. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - 33X04XA5AT (Lactic Acid) RN - EC 1.1.1.27 (L-Lactate Dehydrogenase) RN - 0 (Biomarkers) SB - IM MH - Humans MH - Female MH - Retrospective Studies MH - *Lactic Acid/blood/analysis MH - *Mesenteric Ischemia/surgery/blood MH - Male MH - *L-Lactate Dehydrogenase/blood/analysis MH - Biomarkers/blood/analysis MH - Aged MH - Middle Aged MH - Aged, 80 and over PMC - PMC13707032 OTO - NOTNLM OT - acute mesenteric ischemia OT - lactate OT - lactate dehydrogenase OT - small bowel COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/15 CRDT- 2026/10/27 10:42 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - medicina62101997 [pii] AID - medicina-62-01997 [pii] AID - 10.3390/medicina62101997 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 15;62(10):1997. doi: 10.3390/medicina62101997. PMID- 42942527 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 15 TI - Patient-Reported Tolerability, Treatment Initiation, and Discontinuation of Postoperative Progestin Therapy After Cesarean Scar Repair in Women Planning Pregnancy. LID - 10.3390/medicina62101996 [doi] LID - 1996 AB - Background and Objectives: Cesarean scar disorder (CSDi) may require surgical repair in women with a large niche and thin residual myometrium. At our center, temporary postoperative progestin therapy is recommended to suppress cyclic endometrial proliferation and menstrual activity at the freshly reconstructed scar during early wound healing; whether this improves structural healing is unknown. We evaluated patient-reported treatment initiation, discontinuation, tolerability, bleeding patterns, mood-related symptoms, sexual behavior, and previous hormonal treatment experience in women planning pregnancy. Materials and Methods: This retrospective single-center cohort study included 74 women who underwent laparoscopic-assisted cesarean scar repair between 2020 and 2023. Seventy completed questionnaire follow-ups between March and October 2024 (response rate 94.6%). Hormonal exposure was patient-reported and included desogestrel, dienogest, or an unspecified progestin. Analyses were primarily descriptive and used item-specific denominators. Results: Among women with valid postoperative treatment information, 43/55 (78.2%) reported progestin use. Very-high motivation or a pregnancy-related motivation response was selected by 43/59 women (72.9%). Premature discontinuation because of adverse effects was reported by 15/61 (24.6%), and prolonged vaginal bleeding >14 days by 15/64 (23.4%). Mean perceived adverse-effect intensity was 3.43 (SD 2.99) on a 10-point scale. Fatigue, irritability, and depressed mood were the most frequently reported mood-related symptoms. Among women comparing the postoperative regimen with previous hormonal preparations, 20/32 (62.5%) reported no relevant difference in tolerability. Conclusions: Most women with available treatment information initiated postoperative progestin therapy, and motivation remained substantial despite an active desire for pregnancy. A relevant subgroup reported adverse-effect-related discontinuation or prolonged bleeding. These findings support careful pretreatment counseling and shared decision-making. The effects of postoperative hormonal suppression on anatomical healing and reproductive outcomes remain uncertain. FAU - Born, Tilman AU - Born T AUID- ORCID: 0009-0006-3771-8528 AD - Department of Gynecology and Obstetrics, Hospital St. Hedwig, University of Regensburg, Steinmetzstrasse 1-3, 93049 Regensburg, Germany. FAU - Deglmann, Josef AU - Deglmann J AD - Department of Gynecology and Obstetrics, Hospital St. Hedwig, University of Regensburg, Steinmetzstrasse 1-3, 93049 Regensburg, Germany. FAU - Pohle-Ruffer, Marie AU - Pohle-Ruffer M AUID- ORCID: 0009-0002-3301-8058 AD - Department of Gynecology and Obstetrics, Hospital St. Hedwig, University of Regensburg, Steinmetzstrasse 1-3, 93049 Regensburg, Germany. FAU - Rathberger, Katharina AU - Rathberger K AD - Department of Gynecology and Obstetrics, Hospital St. Hedwig, University of Regensburg, Steinmetzstrasse 1-3, 93049 Regensburg, Germany. FAU - Kappelmeyer, Maurice AU - Kappelmeyer M AUID- ORCID: 0000-0002-9209-7537 AD - Department of Gynecology and Obstetrics, Hospital St. Hedwig, University of Regensburg, Steinmetzstrasse 1-3, 93049 Regensburg, Germany. FAU - Koninger, Angela AU - Koninger A AD - Department of Gynecology and Obstetrics, Hospital St. Hedwig, University of Regensburg, Steinmetzstrasse 1-3, 93049 Regensburg, Germany. FAU - Vural, Mehmet AU - Vural M AD - Department of Gynecology and Obstetrics, Hospital St. Hedwig, University of Regensburg, Steinmetzstrasse 1-3, 93049 Regensburg, Germany. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - 0 (Progestins) SB - IM MH - Humans MH - Female MH - Adult MH - *Progestins/therapeutic use/adverse effects MH - Retrospective Studies MH - *Cicatrix/surgery/drug therapy MH - *Cesarean Section/adverse effects MH - Pregnancy MH - Cohort Studies MH - Surveys and Questionnaires MH - Patient Reported Outcome Measures MH - Postoperative Care/methods PMC - PMC13707190 OTO - NOTNLM OT - cesarean scar disorder OT - desogestrel OT - fertility OT - isthmocele OT - patient-reported outcomes OT - progestin OT - treatment discontinuation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/15 CRDT- 2026/10/27 10:42 PHST- 2026/08/25 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - medicina62101996 [pii] AID - medicina-62-01996 [pii] AID - 10.3390/medicina62101996 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 15;62(10):1996. doi: 10.3390/medicina62101996. PMID- 42942523 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 15 TI - Why Outcomes Diverge in Chronic Hepatitis B with Steatotic Liver Disease: Lessons from Within-Study Contrasts. LID - 10.3390/medicina62101992 [doi] LID - 1992 AB - Background and Objectives: The "friend or foe" debate in chronic hepatitis B (CHB) with steatotic liver disease (SLD) partly arises from treating hepatic fat, metabolic burden, and steatohepatitis as one exposure and interpreting different outcomes together. Materials and Methods: This narrative review applies an exposure-stage-context framework to literature identified in PubMed and Web of Science through 21 September 2026. We synthesized outcome evidence from 119 reports, including 14 detailed examples, using within-study comparisons of exposures, outcomes, and patient subgroups. Results: Within individual cohorts, associations with the same outcome could diverge: steatosis or an SLD diagnosis was associated with lower hepatocellular carcinoma (HCC) or mortality risk, whereas greater metabolic burden was associated with higher risk. Favorable antigen-related associations also coexisted with adverse fibrosis trajectories. Steatohepatitis was associated with more severe fibrosis and liver-related events, while metabolic abnormalities and prolonged glycemic exposure were associated with adverse hepatic outcomes. Studies of metabolic dysfunction-associated steatotic liver disease (MASLD) reported greater cardiovascular risk, and a study of hepatitis B virus-positive patients with nonalcoholic fatty liver disease (NAFLD) reported more prevalent kidney disease. Baseline fibrosis, patient selection, and antiviral treatment shape these associations; lower relative risks can coexist with a high absolute event burden. Conclusions: Current hepatic fat, cumulative metabolic exposure, and established injury therefore carry different prognostic meanings. Their separate assessment helps explain part of the apparent paradox, while adverse steatosis findings and discrepancies in similar settings remain unresolved. The evidence is predominantly observational. FAU - Yang, Xiaoya AU - Yang X AUID- ORCID: 0009-0000-0957-683X AD - Department of Gastroenterology, Beijing Ditan Hospital, Capital Medical University, No. 8 Jingshun East Street, Chaoyang District, Beijing 100015, China. FAU - Wang, Ziteng AU - Wang Z AUID- ORCID: 0000-0002-1543-1933 AD - Department of Gastroenterology, Beijing Ditan Hospital, Capital Medical University, No. 8 Jingshun East Street, Chaoyang District, Beijing 100015, China. FAU - Wei, Hongshan AU - Wei H AUID- ORCID: 0000-0001-8893-653X AD - Department of Gastroenterology, Beijing Ditan Hospital, Capital Medical University, No. 8 Jingshun East Street, Chaoyang District, Beijing 100015, China. AD - HBV Infection, Clinical Cure and Immunology Laboratory for Clinical Medicine, Capital Medical University, No. 8 Jingshun East Street, Chaoyang District, Beijing 100015, China. LA - eng PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - *Hepatitis B, Chronic/complications/physiopathology MH - *Fatty Liver/complications MH - *Non-alcoholic Fatty Liver Disease/complications MH - Liver Neoplasms MH - Carcinoma, Hepatocellular PMC - PMC13706878 OTO - NOTNLM OT - cardiometabolic risk OT - chronic hepatitis B OT - hepatic steatosis OT - hepatocellular carcinoma OT - metabolic dysfunction-associated steatotic liver disease OT - steatotic liver disease COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/15 CRDT- 2026/10/27 10:42 PHST- 2026/09/01 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - medicina62101992 [pii] AID - medicina-62-01992 [pii] AID - 10.3390/medicina62101992 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 15;62(10):1992. doi: 10.3390/medicina62101992. PMID- 42942519 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 14 TI - Integrative Multi-Database Bioinformatics Characterization of FANCE in Hepatocellular Carcinoma: Prognostic, Epigenetic, Immune, and ncRNA Associations. LID - 10.3390/medicina62101988 [doi] LID - 1988 AB - Background and Objectives: The Fanconi anemia complementation group E (FANCE) is a protein component of the Fanconi anemia (FA) DNA repair complex and contributes to genome integrity. However, its molecular and clinical relevance in liver hepatocellular carcinoma (LIHC) remains incompletely understood. This study characterized the FANCE expression, prognosis, epigenetics, immune responses, noncoding RNA (ncRNA) networks, and other related molecular processes in LIHC. Materials and Methods: An integrative multi-database bioinformatics analysis was conducted using public databases and web-based platforms. FANCE expression, DNA methylation, co-expression profiles, and functional enrichment; immune cell infiltration; miRNA-ncRNA, chemical-gene, and protein-protein interactions; treatment response; and survival were evaluated. Results: FANCE expression was higher in LIHC than in non-tumor liver tissues, and this was linked to shorter survival duration. Promoter methylation was lower in LIHC, and select CpG sites were related to FANCE expression and survival. Immune analyses identified heterogeneous relationships between FANCE and inferred immune cell populations. hsa-miR-101-3p was reduced in LIHC, negatively correlated with FANCE, and linked to prolonged survival; subsequent network analysis identified candidate lncRNAs, small ncRNAs, circRNA-linked genes, and pseudogenes. FANCE-related transcriptional profiles were enriched in the cell cycle, DNA replication, FA, and other DNA-repair processes, whereas negatively correlated genes were mainly linked to hepatic metabolic functions. Exploratory treatments identified drug-dependent survival patterns related to FANCE expression and elevated levels in responders to specific immune-checkpoint inhibitors, although the discriminatory performance was modest. Conclusions: Increased FANCE expression in LIHC was linked to molecular features involving DNA methylation, immune cell composition, ncRNA-related interactions, and DNA repair programs as well as shorter survival. These findings provide an integrated molecular context for FANCE in LIHC but do not establish a causal regulation or therapeutic predictive value. Independent clinical and experimental validation studies are warranted to determine its biological and clinical significance. FAU - Kim, Yeong Chae AU - Kim YC AD - Department of Anatomy, College of Medicine, Dongguk University, Gyeongju 38066, Republic of Korea. FAU - Lee, Kyu-Shik AU - Lee KS AUID- ORCID: 0000-0001-5656-468X AD - Department of Pharmacology, College of Medicine, Dongguk University, Gyeongju 38066, Republic of Korea. FAU - Kim, Hye-Ran AU - Kim HR AUID- ORCID: 0000-0002-9399-0503 AD - Department of Biomedical Laboratory Science, Dong-Eui Institute of Technology, Busan 47230, Republic of Korea. FAU - Kim, Jongwan AU - Kim J AUID- ORCID: 0000-0001-6183-3100 AD - Department of Pharmacology, College of Medicine, Dongguk University, Gyeongju 38066, Republic of Korea. LA - eng GR - Dong-Eui Institute of Technology/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - 0 (RNA, Untranslated) SB - IM MH - Humans MH - *Carcinoma, Hepatocellular/genetics/immunology MH - *Liver Neoplasms/genetics/immunology MH - RNA, Untranslated/analysis/genetics MH - *Computational Biology/methods MH - Prognosis MH - Epigenesis, Genetic MH - DNA Methylation PMC - PMC13706819 OTO - NOTNLM OT - DNA methylation OT - Fanconi anemia OT - Fanconi anemia complementation group E OT - anticancer-drug response OT - immune infiltration OT - liver hepatocellular carcinoma OT - ncRNA networks COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/14 CRDT- 2026/10/27 10:42 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - medicina62101988 [pii] AID - medicina-62-01988 [pii] AID - 10.3390/medicina62101988 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 14;62(10):1988. doi: 10.3390/medicina62101988. PMID- 42942518 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 14 TI - Cardiac Denervation and Reinnervation After Heart Transplantation: Assessment and Clinical Impact. LID - 10.3390/medicina62101987 [doi] LID - 1987 AB - The heart is innervated by an intricate network involving sympathetic, parasympathetic, and sensory afferent pathways, which together regulate heart rate, contractility, and vascular tone in response to physiological demands. This innervation is anatomically organized along the neurocardiac axis, from the intrinsic cardiac ganglia to central autonomic centers, and molecularly shaped during development by neurotrophic and repulsive signaling cues. Orthotopic heart transplantation inevitably severs all afferent and efferent neural connections between the donor heart and the recipient, producing a state of complete cardiac denervation that manifests as resting tachycardia, blunted heart rate variability, and abolished reflex chronotropic responses. Over time, a variable degree of sympathetic and, less consistently, parasympathetic reinnervation may occur in a subset of recipients, predominantly in the anterior wall of the left ventricle, typically beginning after the first post-transplant year and progressing gradually, with a characteristic base-to-apex and epicardium-to-endocardium pattern that often remains incomplete even at long-term follow-up. Reinnervation is currently assessed through complementary non-invasive approaches: heart rate variability analysis provides an indirect functional readout of autonomic modulation, while nuclear imaging techniques (including (123)I-metaiodobenzylguanidine scintigraphy and (11)C-metahydroxyephedrine positron emission tomography) allow direct visualization and quantification of regional sympathetic nerve density and integrity. The extent and pattern of reinnervation appear clinically relevant, as denervation and its recovery have been associated with exercise capacity, arrhythmic risk, silent myocardial ischemia, and long-term prognosis after transplantation, although findings across studies remain heterogeneous. This narrative review summarizes current knowledge of cardiac autonomic anatomy and neurotransmission, the molecular determinants of innervation patterning, the physiological consequences of heart transplant-related denervation, the evidence on post-transplant reinnervation and its non-invasive assessment, and the impact of reinnervation on clinical outcomes. FAU - Galeone, Antonella AU - Galeone A AUID- ORCID: 0000-0001-5965-6571 AD - Department of Surgery, Dentistry, Pediatrics and Gynecology, Division of Cardiac Surgery, University of Verona, 372126 Verona, Italy. FAU - Mazzeo, Gina AU - Mazzeo G AUID- ORCID: 0009-0003-1323-0366 AD - Department of Surgery, Dentistry, Pediatrics and Gynecology, Division of Cardiac Surgery, University of Verona, 372126 Verona, Italy. FAU - Moretti, Michele AU - Moretti M AUID- ORCID: 0000-0003-0763-5984 AD - Division of Cardiology, Santa Chiara Hospital, ASUIT, 38123 Trento, Italy. FAU - Munari, Enrico AU - Munari E AUID- ORCID: 0000-0002-3867-6685 AD - Department of Public Health and Diagnsotics, Division of Pathology, University of Verona, 37126 Verona, Italy. FAU - Gambaro, Alessia AU - Gambaro A AUID- ORCID: 0000-0001-8341-1427 AD - Division of Cardiology, Azienda Ospedaliera Universitaria Integrate of Verona, 37126 Verona, Italy. FAU - Capovilla, Teresa Maria AU - Capovilla TM AUID- ORCID: 0000-0002-9153-4686 AD - Division of Cardiology, Santa Maria Del Carmine Hospital, ASUIT, 38068 Rovereto, Italy. FAU - Brunelli, Matteo AU - Brunelli M AD - Department of Public Health and Diagnsotics, Division of Pathology, University of Verona, 37126 Verona, Italy. FAU - Luciani, Giovanni Battista AU - Luciani GB AUID- ORCID: 0000-0003-0931-018X AD - Department of Surgery, Dentistry, Pediatrics and Gynecology, Division of Cardiac Surgery, University of Verona, 372126 Verona, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - *Heart Transplantation/adverse effects/methods MH - *Heart/innervation/physiopathology MH - Heart Rate/physiology MH - *Denervation/methods PMC - PMC13706920 OTO - NOTNLM OT - cardiac allograft vasculopathy OT - cardiac denervation OT - cardiac reinnervation OT - heart rate variability OT - heart transplantation OT - myocardial scintigraphy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/14 CRDT- 2026/10/27 10:42 PHST- 2026/08/27 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - medicina62101987 [pii] AID - medicina-62-01987 [pii] AID - 10.3390/medicina62101987 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 14;62(10):1987. doi: 10.3390/medicina62101987. PMID- 42942514 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 14 TI - Supramalleolar Osteotomy for Varus Ankle Osteoarthritis: A Contemporary Narrative Review. LID - 10.3390/medicina62101983 [doi] LID - 1983 AB - Background and Objectives: Varus ankle osteoarthritis (OA) is most commonly post-traumatic and is characterized by asymmetric degeneration and medial overload of the tibiotalar joint. Supramalleolar osteotomy (SMOT) is a joint-preserving procedure that aims to restore mechanical alignment, redistribute joint contact forces, and delay, or potentially avoid, total ankle replacement (TAR) or ankle arthrodesis (AA). However, the optimal choice of technique and amount of correction remain debated, particularly in complex multiplanar deformities. Materials and Methods: This narrative review summarizes current evidence on SMOT for varus ankle OA. Relevant clinical, radiographic, biomechanical, imaging, and surgical studies were selected and synthesized narratively, with particular emphasis on deformity classification, location of the center of rotation of angulation (CORA), joint congruency, surgical technique, clinical outcomes, complications, and treatment planning. Results: SMOT provides reliable pain relief and functional improvement in patients with Takakura-Tanaka stage 2-3A ankle OA, with reported mid-term survival rates of 88-93%. The choice of technique should be guided by the location of the deformity, the degree of talar tilt, and overall limb alignment. In selected cases, a concomitant fibular osteotomy improves talar reduction and medial unloading. Three-dimensional planning and patient-specific instrumentation may improve the accuracy of correction. Conclusions: SMOT is an effective joint-preserving treatment for varus ankle OA, provided that the deformity is accurately analyzed and patients are carefully selected. This review proposes a simplified, stepwise treatment algorithm, supported by illustrations, to help surgeons select the most appropriate osteotomy technique according to the deformity pattern. FAU - Caravelli, Silvio AU - Caravelli S AUID- ORCID: 0000-0001-9092-225X AD - U.O. Ortopedia Bentivoglio, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy. FAU - Di Ponte, Marco AU - Di Ponte M AUID- ORCID: 0000-0002-1022-7167 AD - U.O. Ortopedia Bentivoglio, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy. AD - Department of Biomedical and Neuromator Sciences (DIBINEM), University of Bologna, 40126 Bologna, Italy. FAU - Berveglieri, Luca AU - Berveglieri L AUID- ORCID: 0000-0002-0778-9660 AD - U.O. Ortopedia Bentivoglio, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy. FAU - Gardini, Giammarco AU - Gardini G AUID- ORCID: 0000-0003-3963-074X AD - U.O. Ortopedia Bentivoglio, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy. FAU - Zmerly, Hassan AU - Zmerly H AUID- ORCID: 0000-0003-3353-1759 AD - Department of Life Sciences, Health, and Health Professions, Link Campus University, 00165 Rome, Italy. FAU - Cassanelli, Edoardo AU - Cassanelli E AUID- ORCID: 0009-0000-5461-4778 AD - U.O. Ortopedia Bentivoglio, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy. FAU - Vocale, Emanuele AU - Vocale E AD - U.O. Ortopedia Bentivoglio, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy. FAU - Zaffagnini, Stefano AU - Zaffagnini S AUID- ORCID: 0000-0002-2941-1407 AD - Clinica Ortopedica e Traumatologica II, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy. FAU - Mosca, Massimiliano AU - Mosca M AUID- ORCID: 0000-0002-3283-8735 AD - U.O. Ortopedia Bentivoglio, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - *Osteotomy/methods MH - *Osteoarthritis/surgery MH - *Ankle Joint/surgery/abnormalities/physiopathology PMC - PMC13707240 OTO - NOTNLM OT - CORA OT - SMOT OT - ankle arthrodesis OT - malalignment OT - surgical treatment OT - total ankle replacement OT - treatment algorithm COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/14 CRDT- 2026/10/27 10:42 PHST- 2026/07/29 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - medicina62101983 [pii] AID - medicina-62-01983 [pii] AID - 10.3390/medicina62101983 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 14;62(10):1983. doi: 10.3390/medicina62101983. PMID- 42942512 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 14 TI - High-Flow Nasal Cannula in Adult Acute Care: Physiological Mechanisms, Clinical Evidence, and Persistent Misconceptions. LID - 10.3390/medicina62101981 [doi] LID - 1981 AB - High-flow nasal cannula (HFNC) oxygen therapy is widely used in emergency, intensive care, perioperative, and procedural settings, but it is often conceptually assimilated to non-invasive positive-pressure ventilation (NIPPV). This interpretation is physiologically misleading. HFNC is an open high-flow oxygen system whose main effects derive from dead-space washout, improved stability of inspired oxygen fraction (FiO(2)), heated humidification, patient comfort, and only modest flow-dependent positive airway pressure. Its open interface also facilitates eating, drinking, speaking, and secretion clearance. Clinical evidence supports HFNC mainly in selected patients with acute hypoxemic respiratory failure, where it may reduce intubation compared with conventional oxygen therapy. However, evidence is more heterogeneous in hypercapnic respiratory failure, cardiogenic pulmonary edema, immunocompromised patients, obesity, and settings requiring substantial pressure support or ventilatory unloading. NIPPV remains preferred in acidotic hypercapnic COPD exacerbations, while CPAP or NIPPV is indicated in selected pressure-responsive conditions such as cardiogenic pulmonary edema. Persistent excessive effort or clinical deterioration requires timely assessment for invasive ventilation. HFNC should therefore be regarded as optimized high-flow oxygen therapy rather than as "non-invasive ventilation through nasal prongs." Recognizing this distinction may improve patient selection, guide escalation, and avoid overinterpretation of its clinical effects. FAU - Orso, Daniele AU - Orso D AUID- ORCID: 0000-0001-7136-0343 AD - Department of Anesthesia and Intensive Care Medicine, "Santa Maria della Misericordia", Udine University Hospital, Azienda Sanitaria Universitaria Friuli Centrale (ASUFC), 33100 Udine, Italy. FAU - Meroi, Francesco AU - Meroi F AUID- ORCID: 0000-0002-7989-1605 AD - Department of Anesthesia and Intensive Care Medicine, "Santa Maria della Misericordia", Udine University Hospital, Azienda Sanitaria Universitaria Friuli Centrale (ASUFC), 33100 Udine, Italy. FAU - Brussa, Alessandro AU - Brussa A AD - Department of Anesthesia and Intensive Care Medicine, "Santa Maria della Misericordia", Udine University Hospital, Azienda Sanitaria Universitaria Friuli Centrale (ASUFC), 33100 Udine, Italy. FAU - Ladu, Miriam AU - Ladu M AD - Department of Medicine (DMED), University of Udine, 33100 Udine, Italy. FAU - Spagnolello, Ornella AU - Spagnolello O AUID- ORCID: 0000-0002-6201-389X AD - Department of Medicine (DMED), University of Udine, 33100 Udine, Italy. FAU - Comisso, Irene AU - Comisso I AD - Department of Anesthesia and Intensive Care Medicine, "Santa Maria della Misericordia", Udine University Hospital, Azienda Sanitaria Universitaria Friuli Centrale (ASUFC), 33100 Udine, Italy. FAU - Federici, Nicola AU - Federici N AD - Department of Anesthesia and Intensive Care Medicine, "Santa Maria della Misericordia", Udine University Hospital, Azienda Sanitaria Universitaria Friuli Centrale (ASUFC), 33100 Udine, Italy. FAU - Della Rocca, Giorgio AU - Della Rocca G AD - Department of Medicine (DMED), University of Udine, 33100 Udine, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - *Cannula/standards MH - *Oxygen Inhalation Therapy/methods/instrumentation/standards MH - Respiratory Insufficiency/therapy MH - Noninvasive Ventilation/methods MH - Adult PMC - PMC13706988 OTO - NOTNLM OT - HFNC OT - high-flow nasal cannula OT - non-invasive respiratory support OT - oxygen therapy OT - physiology OT - respiratory failure COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/14 CRDT- 2026/10/27 10:42 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - medicina62101981 [pii] AID - medicina-62-01981 [pii] AID - 10.3390/medicina62101981 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 14;62(10):1981. doi: 10.3390/medicina62101981. PMID- 42942508 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 13 TI - Protective Effects of Prophylactic and Therapeutic Erythromycin Administration in Ovarian Ischemia-Reperfusion Injury in Rats. LID - 10.3390/medicina62101977 [doi] LID - 1977 AB - Background and Objectives: Ovarian ischemia-reperfusion (I/R) injury involves oxidative stress, inflammation, and structural tissue damage that may impair ovarian function. This study investigated the effects of prophylactic and therapeutic erythromycin administration on biochemical and histopathological injury in a rat model of ovarian I/R. Materials and Methods: Forty adult female Wistar albino rats were divided into five groups (n = 8 per group): sham (SG), I/R, erythromycin alone (E), prophylactic erythromycin plus I/R (I/R+ERP), and therapeutic erythromycin plus I/R (I/R+ERT). Ovarian ischemia was induced by clamping the right ovarian vascular pedicle for 3 h, followed by 3 h of reperfusion. Erythromycin (50 mg/kg, intraperitoneally) was administered 30 min before ischemia in the I/R+ERP group and at the onset of reperfusion in the I/R+ERT group. Inflammatory and oxidative stress markers in serum and ovarian tissue, serum anti-Mullerian hormone (AMH) levels, and ovarian hemorrhage and necrosis were evaluated. Results: Compared with the sham group, ovarian I/R increased inflammatory and oxidative stress markers and histopathological injury, and decreased serum AMH and ovarian tissue glutathione (GSH) and superoxide dismutase (SOD) levels. Compared with the untreated I/R group, both erythromycin regimens were associated with lower serum and ovarian tissue tumor necrosis factor-alpha (TNF-alpha) and malondialdehyde (MDA) levels and lower ovarian necrosis scores. The I/R+ERT group also had lower ovarian tissue interleukin-1beta (IL-1beta), higher ovarian tissue GSH and SOD levels, and a lower hemorrhage score than the untreated I/R group. In the direct comparison between the two erythromycin regimens, serum MDA was lower (false discovery rate [FDR]-adjusted q < 0.001), ovarian tissue SOD was higher (q = 0.014), and the ovarian necrosis score was lower (Holm-adjusted p < 0.001) in the I/R+ERT group. Direct comparisons for serum TNF-alpha (q = 0.055), ovarian tissue IL-1beta (q = 0.127), and ovarian tissue GSH (q = 0.105) did not meet the FDR-adjusted significance threshold. The hemorrhage score did not differ significantly between the two erythromycin regimens (Holm-adjusted p = 0.057). Neither regimen significantly increased serum AMH compared with the untreated I/R group. Conclusions: Erythromycin attenuated several biochemical and histopathological indicators of acute experimental ovarian I/R injury. Administration at the onset of reperfusion was associated with more favorable findings than prophylactic administration for selected outcomes. These short-term findings do not establish preservation of ovarian reserve or fertility, or clinical efficacy in ovarian torsion. FAU - Akyuz, Sumeyye AU - Akyuz S AD - Department of Medical Microbiology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24002, Turkey. FAU - Tas, Necip Gokhan AU - Tas NG AD - Department of Medical Microbiology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24002, Turkey. FAU - Gunay, Murat AU - Gunay M AD - Department of Biochemistry, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24002, Turkey. FAU - Kurt, Nezahat AU - Kurt N AUID- ORCID: 0000-0002-1685-5332 AD - Department of Biochemistry, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24002, Turkey. AD - Research and Development (R&D) Laboratory, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24002, Turkey. FAU - Altindag, Fikret AU - Altindag F AD - Department of Histology and Embryology, Faculty of Medicine, Van Yuzuncu Yil University, Van 65080, Turkey. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - 63937KV33D (Erythromycin) RN - GAN16C9B8O (Glutathione) RN - EC 1.15.1.1 (Superoxide Dismutase) RN - 80497-65-0 (Anti-Mullerian Hormone) SB - IM MH - Animals MH - Female MH - *Erythromycin/therapeutic use/pharmacology/administration & dosage MH - *Reperfusion Injury/drug therapy/prevention & control MH - Rats MH - Rats, Wistar MH - *Ovary/blood supply/drug effects MH - Oxidative Stress/drug effects MH - Disease Models, Animal MH - Glutathione/analysis/blood MH - Superoxide Dismutase/blood/analysis MH - Anti-Mullerian Hormone/blood/analysis PMC - PMC13707145 OTO - NOTNLM OT - erythromycin OT - inflammation OT - ovarian ischemia-reperfusion injury OT - ovary OT - oxidative stress OT - rat COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/13 CRDT- 2026/10/27 10:42 PHST- 2026/09/06 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - medicina62101977 [pii] AID - medicina-62-01977 [pii] AID - 10.3390/medicina62101977 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 13;62(10):1977. doi: 10.3390/medicina62101977. PMID- 42942506 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 13 TI - Competing Mortality, Not Breast-Cancer Mortality, Explains the Survival Deficit of Breast Cancer After a Prior Non-Breast Cancer: A SEER Competing-Risks Analysis. LID - 10.3390/medicina62101975 [doi] LID - 1975 AB - Background and Objectives: Women who develop breast cancer after a previous cancer of another site have poorer overall survival, yet it is unsettled whether the breast cancer itself is more lethal or whether the deficit reflects competing causes of death in an older, previously treated population. We decomposed this survival deficit by cause of death, defining every endpoint identically across groups. Materials and Methods: Using the Surveillance, Epidemiology, and End Results (SEER) database (17 registries, 2010 to 2020), we studied women with invasive breast cancer as a single primary (n = 473,073), as a second or later primary after a non-breast index cancer (second primary breast cancer, SPBC; n = 26,832), and as a second breast primary (n = 40,252). Cause of death was assigned from the SEER cause-of-death-to-site recode, applied identically across cohorts. Cohorts were compared using cause-specific and Fine-Gray models weighted by an inverse-probability propensity score for age, race and ethnicity, subtype, grade, stage, nodal status, year, marital status, income, and rurality. Results: Breast-cancer mortality was not higher in SPBC than in single-primary disease, and was if anything lower (10-year cumulative incidence 12.0 versus 13.6 percent; weighted cause-specific hazard ratio 1.06). The deficit fell almost entirely to competing, non-breast mortality (35.3 versus 13.5 percent). In a five-way decomposition, cardiovascular disease was not the leading competing cause; non-cancer causes and the index cancer each accounted for more deaths. Once patient factors were balanced, cardiovascular and other non-cancer hazards were near unity (1.05 and 1.07), leaving further cancer death as the main excess. Conclusions: A breast cancer arising after a non-breast cancer is not intrinsically more lethal. Its survival deficit is competing mortality, driven mainly by further cancer death and, to a smaller and largely age-explained degree, by cardiovascular and other non-cancer causes. FAU - Zihni, Ismail AU - Zihni I AUID- ORCID: 0000-0002-2133-2106 AD - Department of General Surgery, Faculty of Medicine, Akdeniz University, 07070 Antalya, Turkiye. FAU - Zihni, Burcu AU - Zihni B AD - Department of Cardiology, Antalya City Hospital, 07080 Antalya, Turkiye. FAU - Mutlu, Imren AU - Mutlu I AUID- ORCID: 0000-0003-3427-2023 AD - Department of Radiology, Faculty of Medicine, Akdeniz University, 07070 Antalya, Turkiye. FAU - Saridemir Unal, Demet AU - Saridemir Unal D AD - Department of General Surgery, Faculty of Medicine, Akdeniz University, 07070 Antalya, Turkiye. FAU - Sabuncuoglu, Mehmet Zafer AU - Sabuncuoglu MZ AUID- ORCID: 0000-0002-5549-4023 AD - Department of General Surgery, Faculty of Medicine, Akdeniz University, 07070 Antalya, Turkiye. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - Female MH - *Breast Neoplasms/mortality MH - SEER Program/statistics & numerical data MH - Aged MH - Middle Aged MH - Cause of Death MH - Risk Assessment/methods/statistics & numerical data MH - *Neoplasms, Second Primary/mortality MH - United States/epidemiology MH - Adult MH - Survival Analysis PMC - PMC13706995 OTO - NOTNLM OT - SEER OT - cardiovascular mortality OT - cause of death OT - competing risks OT - second primary breast cancer COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/13 CRDT- 2026/10/27 10:42 PHST- 2026/08/30 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - medicina62101975 [pii] AID - medicina-62-01975 [pii] AID - 10.3390/medicina62101975 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 13;62(10):1975. doi: 10.3390/medicina62101975. PMID- 42942505 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 12 TI - Diagnostic Accuracy of VE1 Immunohistochemistry for BRAF p.V600E in Colorectal Carcinoma: A Systematic Review. LID - 10.3390/medicina62101974 [doi] LID - 1974 AB - Background and Objectives: BRAF p.V600E status has diagnostic, prognostic, and therapeutic relevance in colorectal carcinoma. Molecular assays remain the reference methods, whereas mutation-specific BRAF (VE1 clone) immunohistochemistry offers a rapid tissue-based alternative. We evaluated VE1-molecular concordance and factors affecting diagnostic performance. Materials and Methods: PubMed/MEDLINE, Scopus, and Web of Science were searched in August 2025 and updated on 27 July 2026 for English-language original studies published from 2014 through the update date. Eligible reports applied VE1 and a recognized molecular reference method to the same colorectal carcinoma specimens or a defined overlapping cohort. Study selection and data extraction were performed by one reviewer and independently verified by a second reviewer. Risk of bias and applicability were assessed at the estimate level with QUADAS-3. Because of substantial clinical and methodological heterogeneity, no meta-analysis was performed, and the findings were synthesized descriptively. Results: Of 704 database records, 358 unique records were screened; 38 full-text reports were assessed and 28 were included. Twenty-three reports or defined subcohorts provided complete or clearly reconstructible 2 x 2 comparisons. Sensitivity ranged from 74.6% to 100% and specificity from 66.7% to 100%. Most optimized protocols exceeded 90% for both measures, whereas selected sampling, weak or focal staining, post hoc thresholds, protocol effects, and selective molecular verification reduced reliability. Conclusions: VE1 immunohistochemistry may provide a rapid, locally validated triage method for detecting BRAF p.V600E in colorectal carcinoma. However, substantial heterogeneity and recurrent risk-of-bias concerns limit the generalizability of the available estimates and do not support universal substitution for molecular testing. Equivocal, technically inadequate, or clinically discordant results require molecular clarification. FAU - Susu, Ovidiu-Ioan AU - Susu OI AD - Faculty of Medicine, George Emil Palade University of Medicine, Pharmacy, Sciences and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540139 Targu Mures, Romania. AD - General Surgery Clinic No. 1, County Emergency Clinical Hospital of Targu Mures, 50 Gheorghe Marinescu Street, 540136 Targu Mures, Romania. AD - Doctoral School of Medicine and Pharmacy, George Emil Palade University of Medicine, Pharmacy, Sciences and Technology of Targu Mures, 540142 Targu Mures, Romania. FAU - Botoncea, Marian AU - Botoncea M AUID- ORCID: 0000-0002-7608-4306 AD - Faculty of Medicine, George Emil Palade University of Medicine, Pharmacy, Sciences and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540139 Targu Mures, Romania. AD - General Surgery Clinic No. 1, County Emergency Clinical Hospital of Targu Mures, 50 Gheorghe Marinescu Street, 540136 Targu Mures, Romania. FAU - Serac, Gabriel AU - Serac G AD - General Surgery Clinic No. 1, County Emergency Clinical Hospital of Targu Mures, 50 Gheorghe Marinescu Street, 540136 Targu Mures, Romania. FAU - Beleaua, Marius-Alexandru AU - Beleaua MA AUID- ORCID: 0000-0002-0175-7588 AD - Faculty of Medicine, George Emil Palade University of Medicine, Pharmacy, Sciences and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540139 Targu Mures, Romania. AD - Pathology Department, County Emergency Clinical Hospital of Bistrita, 43 General Grigore Balan Boulevard, 420094 Bistrita-Nasaud, Romania. FAU - Sipos, Tamas-Csaba AU - Sipos TC AUID- ORCID: 0009-0003-2249-3918 AD - Faculty of Medicine, George Emil Palade University of Medicine, Pharmacy, Sciences and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540139 Targu Mures, Romania. AD - Pathology Department, County Emergency Clinical Hospital of Targu Mures, 50 Gheorghe Marinescu Street, 540136 Targu Mures, Romania. FAU - Taurean, Oana Alexandra AU - Taurean OA AUID- ORCID: 0009-0000-1082-4271 AD - Faculty of Medicine, George Emil Palade University of Medicine, Pharmacy, Sciences and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540139 Targu Mures, Romania. FAU - Molnar, Dragos Calin AU - Molnar DC AD - General Surgery Clinic No. 1, County Emergency Clinical Hospital of Targu Mures, 50 Gheorghe Marinescu Street, 540136 Targu Mures, Romania. FAU - Sipos, Remus-Sebastian AU - Sipos RS AD - Faculty of Medicine, George Emil Palade University of Medicine, Pharmacy, Sciences and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540139 Targu Mures, Romania. FAU - Pap, Zsuzsanna AU - Pap Z AD - Faculty of Medicine, George Emil Palade University of Medicine, Pharmacy, Sciences and Technology of Targu Mures, 38 Gheorghe Marinescu Street, 540139 Targu Mures, Romania. LA - eng PT - Journal Article PT - Review PT - Systematic Review DEP - 20261012 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - EC 2.7.11.1 (Proto-Oncogene Proteins B-raf) RN - EC 2.7.11.1 (BRAF protein, human) SB - IM MH - Humans MH - *Proto-Oncogene Proteins B-raf/genetics/analysis MH - *Colorectal Neoplasms/diagnosis/genetics MH - *Immunohistochemistry/methods/standards MH - Sensitivity and Specificity MH - Mutation PMC - PMC13706911 OTO - NOTNLM OT - BRAF V600E OT - Lynch syndrome OT - VE1 antibody OT - colorectal cancer OT - diagnostic concordance OT - immunohistochemistry OT - mismatch repair deficiency OT - molecular testing COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/12 CRDT- 2026/10/27 10:42 PHST- 2026/08/11 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - medicina62101974 [pii] AID - medicina-62-01974 [pii] AID - 10.3390/medicina62101974 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 12;62(10):1974. doi: 10.3390/medicina62101974. PMID- 42942504 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 12 TI - Crestal Versus Lateral Window Approaches for Maxillary Sinus Floor Augmentation: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. LID - 10.3390/medicina62101973 [doi] LID - 1973 AB - Background and Objectives: Evidence directly comparing crestal and lateral approaches for maxillary sinus floor augmentation remains uncertain. We compared their outcomes and assessed certainty of evidence. Materials and Methods: Randomized trials directly comparing both approaches were included. MEDLINE/PubMed, Embase, and the Cochrane Library were searched from inception to 30 July 2026, supplemented by non-systematic Google Scholar searches. Risk of bias and evidence certainty were assessed using RoB 2 and GRADE. Outcomes were pooled as risk ratios (RRs), mean differences (MDs), or standardized mean differences (SMDs), with 95% confidence intervals (CIs). The protocol was registered in PROSPERO (CRD420261447905). Results: Thirteen randomized trials (17 reports) were included. No statistically significant differences were found for implant failure (RR 0.50, 95% CI 0.10-2.61), membrane perforation (RR 0.63, 95% CI 0.29-1.38), marginal bone loss at 12 months (MD -0.08 mm, 95% CI -0.31-0.15), vertical bone gain at 12 months (MD -0.81 mm, 95% CI -1.94-0.33) or 6 months (MD -1.07 mm, 95% CI -2.50-0.36), or postoperative pain (SMD -1.56, 95% CI -3.31-0.20). Certainty was low to very low. Conclusions: Current evidence does not establish superiority of either approach. Selection should reflect residual bone height, sinus anatomy, treatment objectives, and operator experience. FAU - Lee, Heera AU - Lee H AUID- ORCID: 0009-0002-6937-8231 AD - Department of Medicine, Graduate School, The Catholic University of Korea, Seoul 06591, Republic of Korea. AD - Department of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea. FAU - Lee, Sung-Bin AU - Lee SB AUID- ORCID: 0009-0000-1834-7975 AD - Department of Medicine, Graduate School, The Catholic University of Korea, Seoul 06591, Republic of Korea. FAU - Kim, Na Jin AU - Kim NJ AUID- ORCID: 0000-0001-7280-9579 AD - Medical Library, The Catholic University of Korea, Seoul 06591, Republic of Korea. FAU - Lee, Ji-Eun AU - Lee JE AD - Department of Medicine, Graduate School, The Catholic University of Korea, Seoul 06591, Republic of Korea. AD - Department of Periodontology, Korea University Guro Hospital, Seoul 08308, Republic of Korea. FAU - Park, Jun-Beom AU - Park JB AUID- ORCID: 0000-0002-8915-1555 AD - Department of Medicine, Graduate School, The Catholic University of Korea, Seoul 06591, Republic of Korea. AD - Department of Periodontics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea. AD - Dental Implantology, Graduate School of Clinical Dental Science, The Catholic University of Korea, Seoul 06591, Republic of Korea. LA - eng PT - Journal Article PT - Meta-Analysis PT - Review PT - Systematic Review DEP - 20261012 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - *Maxillary Sinus/surgery MH - Randomized Controlled Trials as Topic MH - *Sinus Floor Augmentation/methods/standards PMC - PMC13706686 OTO - NOTNLM OT - GRADE OT - crestal approach OT - lateral window OT - maxillary sinus floor augmentation OT - meta-analysis OT - randomized controlled trials COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/12 CRDT- 2026/10/27 10:42 PHST- 2026/09/23 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - medicina62101973 [pii] AID - medicina-62-01973 [pii] AID - 10.3390/medicina62101973 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 12;62(10):1973. doi: 10.3390/medicina62101973. PMID- 42942500 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 12 TI - ROS-NLRP3 Inflammasome Axis in Obesity: A Critical Review of Oxidative Stress-Driven Inflammation and Emerging Therapeutic Strategies. LID - 10.3390/medicina62101969 [doi] LID - 1969 AB - Background and Objectives: Obesity is a chronic, low-grade inflammatory state. Oxidative stress and reactive oxygen species (ROS) are increasingly recognized as not just passive byproducts but also signaling contributors to metabolic dysfunction in obesity. Among the inflammatory effectors that interact with ROS, the NOD-, LRR-, and pyrin domain-containing protein 3 (NLRP3) inflammasome has emerged as an important node linking metabolic stress to interleukin (IL)-1beta/IL-18-mediated inflammation and insulin resistance. This review critically examines the ROS-NLRP3 axis as a mechanistic framework for obesity progression, weighing supporting against contradictory evidence and defining translational opportunities. Materials and Methods: A narrative concept-driven review was conducted by searching PubMed, Scopus, and Web of Science throughout August 2026, prioritizing mechanistic, human, and interventional studies of ROS, mitochondrial ROS (mtROS), NLRP3 activation, and obesity, with deliberate inclusion of discordant findings. Results: ROS-dependent NF-kappaB signaling contributes to NLRP3 priming, whereas mtROS, oxidized mitochondrial DNA, and thioredoxin-interacting protein (TXNIP) are consistently implicated in NLRP3 activation and caspase-1-dependent maturation of IL-1beta and IL-18, which are associated with adipose tissue inflammation and impaired insulin signaling. However, loss-of-function studies have yielded inconsistent metabolic protection; human evidence is largely observational, and NLRP3 inhibitors, such as MCC950, carry organ-specific toxicities, indicating context dependence rather than a simple linear pathway. Upstream (mitochondria-targeted antioxidants, TXNIP blockade), core (selective NLRP3 inhibitors), and downstream (IL-1beta/IL-18 blockade) strategies are supported mainly by preclinical or early clinical data; for metformin, SGLT2 inhibitors, and GLP-1 receptor agonists, ROS/NLRP3 modulation is a potentially relevant contributory mechanism. Conclusions: The ROS-NLRP3 axis provides a useful mechanistic framework for obesity-associated inflammation, but human causal evidence is limited, and its contribution is tissue-, stage-, and context-dependent; therefore, human-validated, mechanism-based studies are needed. FAU - Ahn, Sungeun AU - Ahn S AUID- ORCID: 0009-0000-7391-6923 AD - Department of Biopharmaceuticals, Catholic Sangji University, Andong 36686, Republic of Korea. FAU - Park, SangMin AU - Park S AD - Department of Biopharmaceuticals, Catholic Sangji University, Andong 36686, Republic of Korea. FAU - Simu, Shakina Yesmin AU - Simu SY AD - College of Pharmacy, Gachon University, Incheon 21936, Republic of Korea. AD - Department of Oriental Medicinal Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - 0 (NLR Family, Pyrin Domain-Containing 3 Protein) RN - 0 (Reactive Oxygen Species) RN - 0 (Inflammasomes) RN - 0 (NLRP3 protein, human) RN - 0 (Carrier Proteins) RN - 0 (TXNIP protein, human) RN - 0 (Interleukin-18) SB - IM MH - Humans MH - *NLR Family, Pyrin Domain-Containing 3 Protein/metabolism MH - *Reactive Oxygen Species/metabolism MH - *Oxidative Stress/physiology MH - *Obesity/metabolism/physiopathology MH - *Inflammation/metabolism MH - *Inflammasomes/metabolism MH - Animals MH - Carrier Proteins/metabolism MH - Insulin Resistance MH - Signal Transduction MH - Interleukin-18/metabolism PMC - PMC13707055 OTO - NOTNLM OT - NLRP3 inflammasome OT - TXNIP OT - chronic inflammation OT - insulin resistance OT - mitochondrial ROS OT - obesity OT - oxidative stress OT - reactive oxygen species COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/12 CRDT- 2026/10/27 10:42 PHST- 2026/09/01 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - medicina62101969 [pii] AID - medicina-62-01969 [pii] AID - 10.3390/medicina62101969 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 12;62(10):1969. doi: 10.3390/medicina62101969. PMID- 42942498 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 12 TI - Prophylactic Carbetocin Versus Oxytocin and Blood Loss After Vaginal Delivery: Agent Selection and Confounding by Indication in a Retrospective Cohort Study. LID - 10.3390/medicina62101967 [doi] LID - 1967 AB - Background and Objectives: Carbetocin is increasingly used for postpartum hemorrhage (PPH) prophylaxis, but its selection in routine practice may bias comparisons with oxytocin. This observational study examined the association between the agent received and blood loss after vaginal delivery. Materials and Methods: This retrospective cohort included 559 women delivering vaginally in 2022-2023 who received carbetocin 100 mug intravenously (n = 474) or oxytocin 10 IU by infusion (n = 85). Gravimetric blood loss was analyzed using linear (primary), robust and median (co-primary) regression, and propensity score methods. Results: Carbetocin recipients more often underwent induction (63.3% vs. 43.5%), vacuum extraction (26.8% vs. 15.3%), and epidural analgesia (76.2% vs. 29.4%). Blood loss was higher with carbetocin before adjustment (223.7 vs. 180.0 mL, p = 0.003) but not after adjustment (beta 12.72 mL, 95% CI -50.19 to 75.64, p = 0.691; robust p = 0.479; median p = 0.354). Propensity score results conflicted: inverse probability weighting showed higher blood loss with carbetocin (47.84 mL, p = 0.002), whereas matching and trimmed weighting did not. PPH occurred in 2.7% vs. 0% (p = 0.234). Conclusions: Carbetocin was preferentially used in higher-risk labors, indicating confounding by indication. Adjusted blood loss did not differ significantly, although a modest increase could not be excluded. The study was severely underpowered for PPH. FAU - Tsui, Wing Lam AU - Tsui WL AUID- ORCID: 0009-0002-3040-1465 AD - Department of Obstetrics and Gynecology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Tzu Chi University, Hualien 970, Taiwan. FAU - Ding, Dah-Ching AU - Ding DC AUID- ORCID: 0000-0001-5105-068X AD - Department of Obstetrics and Gynecology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Tzu Chi University, Hualien 970, Taiwan. AD - Institute of Medical Sciences, Tzu Chi University, Hualien 970, Taiwan. LA - eng PT - Comparative Study PT - Journal Article PT - Observational Study DEP - 20261012 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - 88TWF8015Y (carbetocin) RN - 0 (Oxytocics) RN - 50-56-6 (Oxytocin) SB - IM MH - Adult MH - Female MH - Humans MH - Pregnancy MH - Cohort Studies MH - *Delivery, Obstetric/methods/adverse effects MH - *Oxytocics/therapeutic use/adverse effects MH - *Oxytocin/analogs & derivatives/therapeutic use/adverse effects/analogs & derivatives MH - *Postpartum Hemorrhage/diagnosis/prevention & control MH - Propensity Score MH - Retrospective Studies PMC - PMC13707158 OTO - NOTNLM OT - carbetocin OT - confounding by indication OT - oxytocin OT - postpartum hemorrhage OT - propensity score OT - vaginal delivery COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/12 CRDT- 2026/10/27 10:42 PHST- 2026/09/24 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - medicina62101967 [pii] AID - medicina-62-01967 [pii] AID - 10.3390/medicina62101967 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 12;62(10):1967. doi: 10.3390/medicina62101967. PMID- 42942490 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 10 TI - Personalized Management of Hydrosalpinx Before IVF: A Narrative Review of Reproductive Outcomes and Treatment Timing. LID - 10.3390/medicina62101959 [doi] LID - 1959 AB - Background and Objectives: Hydrosalpinx is an important and treatable cause of impaired in vitro fertilization (IVF) outcomes, but the optimal intervention and its timing remain uncertain. This narrative review evaluates contemporary evidence to support individualized management before IVF. Materials and Methods: Targeted searches of PubMed/MEDLINE records and publisher websites prioritized systematic reviews, meta-analyses, randomized trials, cohort studies, and international guidelines published from January 2015 to September 2026, while retaining earlier landmark studies. Evidence concerning salpingectomy, laparoscopic proximal tubal occlusion (PTO), aspiration, sclerotherapy, ovarian reserve, and treatment timing was synthesized narratively. Results: Clinically significant communicating hydrosalpinx should generally be treated before embryo transfer. Salpingectomy remains the most established definitive intervention, whereas laparoscopic PTO appears to provide comparable clinical pregnancy outcomes and may reduce tubo-ovarian vascular disruption in selected patients. Evidence regarding ovarian reserve after salpingectomy is conflicting: total oocyte yield is often preserved, although some studies report lower anti-Mullerian hormone, antral follicle count, or ipsilateral follicular recruitment. Aspiration and sclerotherapy may be considered when standard surgery is not feasible, but recurrence and limited randomized evidence restrict routine use. Observational studies do not establish a universally superior sequence of surgery and oocyte retrieval. Conclusions: Management should move from a uniform procedure-centered approach toward patient-centered selection. Reproductive age, ovarian reserve, laterality, pelvic adhesions, surgical complexity, and treatment urgency should guide both the intervention and its timing, while effective hydrosalpinx treatment should precede embryo transfer. FAU - Mihoci, Eduard Cristian AU - Mihoci EC AUID- ORCID: 0009-0003-0791-0613 AD - Department of Mother and Child Medicine, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. AD - Clinical Hospital of Obstetrics and Gynecology "Elena Doamna", 700398 Iasi, Romania. FAU - Socolov, Demetra AU - Socolov D AUID- ORCID: 0000-0002-0979-0200 AD - Department of Mother and Child Medicine, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. AD - Clinical Hospital of Obstetrics and Gynecology "Cuza Voda", 700038 Iasi, Romania. FAU - Socolov, Razvan Vladimir AU - Socolov RV AD - Department of Mother and Child Medicine, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. AD - Clinical Hospital of Obstetrics and Gynecology "Elena Doamna", 700398 Iasi, Romania. FAU - Mihoci, Ana Maria AU - Mihoci AM AD - Department of Mother and Child Medicine, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. AD - Clinical Hospital of Obstetrics and Gynecology "Elena Doamna", 700398 Iasi, Romania. FAU - Nemescu, Dragos AU - Nemescu D AUID- ORCID: 0000-0003-2345-1077 AD - Department of Mother and Child Medicine, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. AD - Clinical Hospital of Obstetrics and Gynecology "Cuza Voda", 700038 Iasi, Romania. LA - eng PT - Journal Article PT - Review DEP - 20261010 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - Female MH - *Fallopian Tube Diseases/therapy/surgery/complications MH - *Fertilization in Vitro/methods MH - Pregnancy MH - Salpingectomy/methods MH - Ovarian Reserve MH - *Precision Medicine/methods MH - Sclerotherapy MH - Treatment Outcome PMC - PMC13706863 OTO - NOTNLM OT - assisted reproductive technology OT - hydrosalpinx OT - in vitro fertilization OT - ovarian reserve OT - personalized treatment OT - proximal tubal occlusion OT - salpingectomy OT - sclerotherapy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/10 CRDT- 2026/10/27 10:42 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - medicina62101959 [pii] AID - medicina-62-01959 [pii] AID - 10.3390/medicina62101959 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 10;62(10):1959. doi: 10.3390/medicina62101959. PMID- 42942485 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 9 TI - Non-Invasive Assessment of Coronary Microvascular Dysfunction: From Flow Quantification to Clinical Decision Making. LID - 10.3390/medicina62101954 [doi] LID - 1954 AB - Coronary microvascular dysfunction (CMD) is a major mechanism of myocardial ischemia and adverse cardiovascular outcomes in patients with angina or dyspnea, both in the absence and in the presence of obstructive coronary artery disease. Because the coronary microcirculation cannot be visualized directly with routine angiography, diagnosis depends on physiological assessment. Non-invasive imaging has moved beyond relative perfusion analysis toward quantification of resting and hyperemic myocardial blood flow and flow reserve. Positron emission tomography (PET) remains the most extensively validated non-invasive method and provides robust absolute flow measurements with strong prognostic evidence. Quantitative stress cardiovascular magnetic resonance (CMR) is rapidly maturing, combining high-resolution perfusion mapping with ventricular and tissue characterization without ionizing radiation. Transthoracic Doppler echocardiography offers an accessible, radiation-free estimate of coronary flow velocity reserve, whereas myocardial contrast echocardiography can assess myocardial blood volume and replenishment kinetics. Dynamic cadmium-zinc-telluride single-photon emission computed tomography and stress computed tomography perfusion extend flow quantification to more widely available platforms, although standardization and outcome validation remain less developed. Across modalities, interpretation requires attention to the adequacy of vasodilator stress, resting hemodynamics, diffuse epicardial atherosclerosis, scar, and technical factors. A reduced flow reserve is not synonymous with isolated microvascular disease and cannot identify acetylcholine-provoked vasospasm. This review provides a clinically oriented framework for selecting, performing, interpreting, and reporting non-invasive CMD testing; examines the evidence, strengths, and limitations of each modality; and defines situations in which invasive coronary function testing remains necessary. Future progress will depend on cross-platform standardization, automated quality control, disease- and sex-specific reference ranges, and prospective trials linking imaging-defined endotypes to effective treatment strategies. FAU - Theofilis, Panagiotis AU - Theofilis P AUID- ORCID: 0000-0001-9260-6306 AD - First Department of Cardiology, National and Kapodistrian University of Athens, Hippokration General Hospital of Athens, 11527 Athens, Greece. FAU - Sakalidis, Athanasios AU - Sakalidis A AUID- ORCID: 0000-0003-3332-803X AD - First Department of Cardiology, National and Kapodistrian University of Athens, Hippokration General Hospital of Athens, 11527 Athens, Greece. AD - Harefield Hospital, Royal Brompton and Harefield Hospitals, Guy's and St Thomas' NHS Foundation Trust, London SE1 7EH, UK. FAU - Dimitriadis, Kyriakos AU - Dimitriadis K AD - First Department of Cardiology, National and Kapodistrian University of Athens, Hippokration General Hospital of Athens, 11527 Athens, Greece. FAU - Karakasis, Paschalis AU - Karakasis P AUID- ORCID: 0000-0002-3561-5713 AD - Second Department of Cardiology, Hippokration General Hospital, Aristotle University of Thessaloniki, Konstantinoupoleos 49, 54642 Thessaloniki, Greece. FAU - Papanikolaou, Aggelos AU - Papanikolaou A AUID- ORCID: 0000-0003-2726-9520 AD - First Department of Cardiology, National and Kapodistrian University of Athens, Hippokration General Hospital of Athens, 11527 Athens, Greece. FAU - Iliakis, Panagiotis AU - Iliakis P AUID- ORCID: 0009-0004-5768-9125 AD - First Department of Cardiology, National and Kapodistrian University of Athens, Hippokration General Hospital of Athens, 11527 Athens, Greece. FAU - Vordoni, Aikaterini AU - Vordoni A AD - First Department of Cardiology, National and Kapodistrian University of Athens, Hippokration General Hospital of Athens, 11527 Athens, Greece. FAU - Vlachakis, Panayotis K AU - Vlachakis PK AUID- ORCID: 0000-0003-0736-4942 AD - First Department of Cardiology, National and Kapodistrian University of Athens, Hippokration General Hospital of Athens, 11527 Athens, Greece. FAU - Tsioufis, Panagiotis AU - Tsioufis P AUID- ORCID: 0000-0002-1100-7430 AD - First Department of Cardiology, National and Kapodistrian University of Athens, Hippokration General Hospital of Athens, 11527 Athens, Greece. FAU - Adamopoulou, Eleni AU - Adamopoulou E AUID- ORCID: 0009-0003-4379-7339 AD - First Department of Cardiology, National and Kapodistrian University of Athens, Hippokration General Hospital of Athens, 11527 Athens, Greece. FAU - Panoulas, Vasileios AU - Panoulas V AUID- ORCID: 0000-0002-9894-9200 AD - Harefield Hospital, Royal Brompton and Harefield Hospitals, Guy's and St Thomas' NHS Foundation Trust, London SE1 7EH, UK. FAU - Aznaouridis, Konstantinos AU - Aznaouridis K AUID- ORCID: 0000-0002-0706-4424 AD - First Department of Cardiology, National and Kapodistrian University of Athens, Hippokration General Hospital of Athens, 11527 Athens, Greece. FAU - Tsioufis, Konstantinos AU - Tsioufis K AUID- ORCID: 0000-0002-7636-6725 AD - First Department of Cardiology, National and Kapodistrian University of Athens, Hippokration General Hospital of Athens, 11527 Athens, Greece. FAU - Tousoulis, Dimitris AU - Tousoulis D AD - First Department of Cardiology, National and Kapodistrian University of Athens, Hippokration General Hospital of Athens, 11527 Athens, Greece. LA - eng PT - Journal Article PT - Review DEP - 20261009 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - *Coronary Circulation/physiology MH - *Microcirculation/physiology MH - Positron-Emission Tomography/methods MH - *Coronary Artery Disease/physiopathology/diagnosis PMC - PMC13707341 OTO - NOTNLM OT - ANOCA OT - CT perfusion OT - INOCA OT - SPECT OT - cardiovascular magnetic resonance OT - coronary microvascular dysfunction OT - echocardiography OT - myocardial blood flow OT - myocardial flow reserve OT - positron emission tomography COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/09 CRDT- 2026/10/27 10:42 PHST- 2026/08/24 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - medicina62101954 [pii] AID - medicina-62-01954 [pii] AID - 10.3390/medicina62101954 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 9;62(10):1954. doi: 10.3390/medicina62101954. PMID- 42942482 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 9 TI - Effects of Stem Cell-Derived Mitochondrial Transfer on H(2)O(2)-Induced Oxidative Stress in COV434 Granulosa Cells. LID - 10.3390/medicina62101951 [doi] LID - 1951 AB - Background and Objectives: Oxidative stress-induced mitochondrial dysfunction contributes to granulosa cell apoptosis and ovarian dysfunction. This study investigated the therapeutic potential of human Wharton's jelly-derived mesenchymal stem cell-derived mitochondria (hWJ-MSCs-mito) in an H(2)O(2)-induced oxidative stress model of COV434 granulosa cells. Materials and Methods: Oxidative stress was induced using increasing concentrations of H(2)O(2), and cell viability was assessed by the WST-8 assay. Isolated hWJ-MSC-mito were labeled with TMRM, transferred into COV434 cells, and confirmed by confocal microscopy. The expression of oxidative stress- and mitochondrial-related genes (CYP19A1, GPX1, HO1, SOD1, and TOMM20) was analyzed by qRT-PCR, whereas proliferative and apoptotic responses were evaluated by PCNA and active caspase-3 immunofluorescence, respectively. Results: Exposure to 1.5 mM H(2)O(2) reduced cell viability by approximately 50%, establishing an oxidative stress model, while 10 microg hWJ-MSCs-mito provided an optimal mitochondrial dose. Transferred mitochondria were successfully internalized by recipient cells and enhanced cell viability, increased PCNA immunoreactivity, and reduced active caspase-3 immunoreactivity. Furthermore, it attenuated H(2)O(2)-induced upregulation of CYP19A1 and HO1, moderately reduced SOD1 expression, preserved GPX1 expression, and showed a modest increase in TOMM20 expression. Conclusions: hWJ-MSC-mito supplementation was associated with improved cell viability, increased PCNA immunoreactivity, reduced active caspase-3 immunoreactivity, and modulation of selected transcriptional responses in H(2)O(2)-treated COV434 cells. These findings support further investigation of mitochondrial supplementation in oxidative stress-induced granulosa cell injury. FAU - Furat, Selenay AU - Furat S AD - Department of Stem Cell, Institute of Health Sciences, Kocaeli University, 41001 Kocaeli, Turkiye. AD - Center for Stem Cell and Gene Therapies Research and Practice, Kocaeli University, 41001 Kocaeli, Turkiye. AD - Department of Histology and Embryology, Faculty of Medicine, Kocaeli University, 41001 Kocaeli, Turkiye. FAU - Altuntas, Candan AU - Altuntas C AUID- ORCID: 0000-0002-2551-1849 AD - Department of Stem Cell, Institute of Health Sciences, Kocaeli University, 41001 Kocaeli, Turkiye. AD - Center for Stem Cell and Gene Therapies Research and Practice, Kocaeli University, 41001 Kocaeli, Turkiye. FAU - Kilic, Kamil Can AU - Kilic KC AD - Department of Stem Cell, Institute of Health Sciences, Kocaeli University, 41001 Kocaeli, Turkiye. AD - Center for Stem Cell and Gene Therapies Research and Practice, Kocaeli University, 41001 Kocaeli, Turkiye. AD - Department of Histology and Embryology, Faculty of Medicine, Kocaeli University, 41001 Kocaeli, Turkiye. LA - eng GR - TAD-2026-5337/Kocaeli Universitesi/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - BBX060AN9V (Hydrogen Peroxide) SB - IM MH - *Oxidative Stress/drug effects/physiology MH - Female MH - Humans MH - *Granulosa Cells/metabolism/drug effects MH - *Mitochondria/metabolism/transplantation MH - *Hydrogen Peroxide/pharmacology MH - *Mesenchymal Stem Cells/metabolism MH - Cell Survival/drug effects MH - Apoptosis/drug effects PMC - PMC13707177 OTO - NOTNLM OT - apoptosis OT - granulosa cells OT - mesenchymal stem cells OT - mitochondrial transfer OT - oxidative stress COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/09 CRDT- 2026/10/27 10:42 PHST- 2026/08/10 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - medicina62101951 [pii] AID - medicina-62-01951 [pii] AID - 10.3390/medicina62101951 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 9;62(10):1951. doi: 10.3390/medicina62101951. PMID- 42942477 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 9 TI - Melatonin in Musculoskeletal and Spine-Related Conditions: A Systematic Review of Preclinical and Clinical Evidence. LID - 10.3390/medicina62101946 [doi] LID - 1946 AB - Background and Objectives: Experimental studies suggest that melatonin can influence antioxidant, inflammatory, mitochondrial, and chronobiological pathways relevant to musculoskeletal degeneration, repair, and selected spine-related conditions, but clinical translation remains uncertain. This systematic review evaluated primary in vitro, animal, and human evidence concerning exogenous parent melatonin in these conditions. Materials and Methods: PubMed/MEDLINE and Europe PMC were searched from inception to 6 August 2026; ClinicalTrials.gov was mapped separately. Eligible reports were peer-reviewed comparative primary investigations. One reviewer performed screening, extraction, and a screen-level methodological limitation assessment, followed by a complete consistency pass; no automation tools were used. Complete articles were consulted where accessible, but access status was not logged prospectively and the exact number accessed cannot be reconstructed reliably. Because quantitative pooling was not appropriate, evidence was synthesized by stream and clinical domain according to PRISMA 2020 and SWiM. The review was not registered, and no standalone protocol was prepared. Results: The searches yielded 3201 records and 1646 unique records after deduplication. Of 437 reports taken forward for retrieval and report-level assessment using available information, 208 were included (23 in vitro, 142 animal, and 43 human reports). Preclinical studies generally reported osteogenic, chondroprotective, antioxidant, anti-inflammatory, mitochondrial, and tissue-repair effects, but findings were not uniformly favorable: high-dose fracture-remodeling impairment and MT2-dependent heterotopic ossification were reported. Human evidence was limited, heterogeneous, and showed frequent screen-level methodological limitations. Formulations, routes, and exposures were non-equivalent. Conclusions: Melatonin is biologically plausible but clinically unproven in the reviewed conditions. No formulation, dose, or route can presently be recommended for disease modification, neurological recovery, or tissue regeneration. FAU - Bisaccia, Michele AU - Bisaccia M AD - Orthopedics and Traumatology Department, Medical School, University of Perugia, S. Maria della Misericordia Hospital, 06123 Perugia, Italy. FAU - Bifarini, Barbara AU - Bifarini B AD - Anaesthesia and Resuscitation, Department of Medicine and Surgery, S. Maria Misericordia Hospital, S. Andrea delle Fratte, 06132 Perugia, Italy. FAU - Ripani, Umberto AU - Ripani U AD - Division of Clinic Anaesthesia, Department of Emergency Hospital Riuniti, Conca Street 71, 60126 Ancona, Italy. FAU - Lucchetta, Lorenzo AU - Lucchetta L AD - Orthopedics and Traumatology Department, Medical School, University of Perugia, S. Maria della Misericordia Hospital, 06123 Perugia, Italy. FAU - Bonanno, Edoardo AU - Bonanno E AD - Orthopedics and Traumatology Department, Medical School, University of Perugia, S. Maria della Misericordia Hospital, 06123 Perugia, Italy. FAU - Siragusano, Marco AU - Siragusano M AD - Orthopedics and Traumatology Department, Medical School, University of Perugia, S. Maria della Misericordia Hospital, 06123 Perugia, Italy. FAU - Di Mitri, Dario AU - Di Mitri D AD - Orthopedics and Traumatology Department, Medical School, University of Perugia, S. Maria della Misericordia Hospital, 06123 Perugia, Italy. FAU - Bronzini, Francesco AU - Bronzini F AD - Orthopedics and Traumatology Department, Medical School, University of Perugia, S. Maria della Misericordia Hospital, 06123 Perugia, Italy. FAU - Rinonapoli, Giuseppe AU - Rinonapoli G AUID- ORCID: 0000-0002-4244-166X AD - Orthopedics and Traumatology Department, Medical School, University of Perugia, S. Maria della Misericordia Hospital, 06123 Perugia, Italy. LA - eng PT - Journal Article PT - Review PT - Systematic Review DEP - 20261009 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - JL5DK93RCL (Melatonin) RN - 0 (Antioxidants) SB - IM MH - *Melatonin/therapeutic use/pharmacology MH - Humans MH - Animals MH - Antioxidants/therapeutic use/pharmacology PMC - PMC13707208 OTO - NOTNLM OT - fracture healing OT - intervertebral disk OT - melatonin OT - osteoarthritis OT - osteoporosis OT - sarcopenia OT - spinal cord injury OT - sports recovery OT - systematic review OT - tendon COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/09 CRDT- 2026/10/27 10:42 PHST- 2026/07/02 00:00 [received] PHST- 2026/08/25 00:00 [revised] PHST- 2026/09/11 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - medicina62101946 [pii] AID - medicina-62-01946 [pii] AID - 10.3390/medicina62101946 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 9;62(10):1946. doi: 10.3390/medicina62101946. PMID- 42942475 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 8 TI - Survival Outcomes in Metaplastic Versus Triple-Negative Invasive Breast Carcinoma: A Propensity Score-Matched Study. LID - 10.3390/medicina62101944 [doi] LID - 1944 AB - Background and Objectives: Metaplastic breast carcinoma (MBC) is a rare, predominantly triple-negative breast cancer subtype with aggressive clinical behaviour. This study compared the clinicopathological characteristics and survival outcomes of MBC with triple-negative invasive breast carcinoma (TN-IBC) and assessed the prognostic value of the neutrophil-to-lymphocyte ratio (NLR). Materials and Methods: Patients with stage I-III MBC or TN-IBC diagnosed between January 2003 and January 2024 were retrospectively reviewed. Among 35 patients with MBC and 103 with TN-IBC, 1:1 propensity score matching by age and TNM stage generated two groups of 35 patients. Disease-free survival (DFS) and overall survival (OS) were evaluated using Kaplan-Meier estimates and Cox proportional hazards models with robust sandwich variance clustered by matched pair. Results: All 70 matched patients had triple-negative disease. During a median follow-up of 7.7 years, recurrence occurred in 22.9% of patients with MBC and 17.1% of those with TN-IBC, while mortality rates were 25.7% and 8.6%, respectively. Five-year DFS rates were 73.9% and 85.2%, respectively; however, MBC was not significantly associated with DFS (HR = 1.70, 95% CI 0.67-4.30; p = 0.262). Five-year OS rates were 79.8% and 94.3%, respectively. Metaplastic histology was associated with a higher mortality hazard (HR = 3.53, 95% CI 1.01-12.41; p = 0.049). Median NLR was higher in MBC than in TN-IBC (2.31 vs. 1.85; p = 0.019). High NLR was associated with poorer OS in the overall matched cohort (HR = 3.36, 95% CI 1.07-10.57; p = 0.038), although NLR was not significantly associated with survival when analysed as a continuous variable or within the MBC subgroup. Conclusions: MBC was associated with inferior OS compared with TN-IBC after propensity score matching and pair-adjusted survival analysis, while DFS did not differ significantly. Elevated NLR was associated with poorer OS in the overall matched cohort but was not validated as an MBC-specific prognostic biomarker. FAU - Caner, Burcu AU - Caner B AUID- ORCID: 0000-0003-1591-3323 AD - Medical Oncology, Aydin Ataturk State Hospital, Aydin 09020, Turkiye. FAU - Ertas, Hulya AU - Ertas H AUID- ORCID: 0000-0002-5872-8825 AD - Medical Oncology, Ali Osman Sonmez Oncology Hospital, Bursa 16040, Turkiye. FAU - Oyucu Orhan, Sibel AU - Oyucu Orhan S AUID- ORCID: 0000-0001-8217-3471 AD - Medical Oncology, Bursa City Hospital, Bursa 16110, Turkiye. FAU - Bahar, Seher AU - Bahar S AD - Radiation Oncology, Balikesir Ataturk City Hospital, Balikesir 10100, Turkiye. LA - eng PT - Journal Article DEP - 20261008 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - Female MH - Propensity Score MH - *Triple Negative Breast Neoplasms/mortality MH - Retrospective Studies MH - Middle Aged MH - Prognosis MH - Aged MH - Disease-Free Survival MH - Adult MH - Proportional Hazards Models MH - Kaplan-Meier Estimate MH - Neoplasm Staging PMC - PMC13707025 OTO - NOTNLM OT - metaplastic breast carcinoma OT - neutrophil-to-lymphocyte ratio OT - overall survival OT - propensity score matching OT - triple-negative breast cancer COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/08 CRDT- 2026/10/27 10:42 PHST- 2026/08/19 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - medicina62101944 [pii] AID - medicina-62-01944 [pii] AID - 10.3390/medicina62101944 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 8;62(10):1944. doi: 10.3390/medicina62101944. PMID- 42942472 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 8 TI - Diagnostic Yield of Internal Medicine Referrals to Rheumatology: A Retrospective Single-Center Study. LID - 10.3390/medicina62101943 [doi] LID - 1943 AB - Background and Objectives: In Turkey, access to adult rheumatology outpatient clinics requires referral from another specialty, mostly internal medicine, and no standardized referral algorithms are in use. We estimated the diagnostic yield of new inflammatory rheumatic disease (IRD) diagnoses among internal medicine referrals who completed rheumatology evaluation and identified referral-stage characteristics associated with a new IRD diagnosis. Materials and Methods: In this retrospective cohort study, 2233 patients referred from internal medicine clinics to rheumatology outpatient clinics between 1 September and 30 November 2022 were screened. Patients with a prior diagnosis of IRD (n = 539) and those who did not attend their scheduled appointment (n = 732) were excluded, leaving 962 patients who completed rheumatology evaluation for analysis. Findings documented at referral were compared between patients with and without a new IRD diagnosis, and associations were examined with a clinically selected multivariable logistic regression model without laboratory variables, because laboratory tests had been ordered selectively. Results: Among the 962 patients who completed rheumatology evaluation, 141 (14.6%) received a new IRD diagnosis, corresponding to 6.3% of all 2233 referrals; the diagnostic status of the 732 patients who did not attend is unknown. In the multivariable model, swollen and tender joints were associated with a new IRD diagnosis (odds ratio [OR] 5.24, 95% confidence interval [CI] 3.17-8.68), whereas sicca symptoms (OR 1.45, 95% CI 0.83-2.55) and constitutional symptoms (OR 1.66, 95% CI 0.76-3.64) were not independently associated. Conclusions: Among patients referred from internal medicine to a tertiary rheumatology clinic who completed evaluation, approximately one in seven received a new IRD diagnosis. Referral appropriateness was not assessed, and the high non-attendance rate limits generalization to all referrals. Swollen and tender joints were the only referral characteristic independently associated with a new diagnosis. Whether structured referral criteria, medical education, or triage strategies improve the yield of referrals is a hypothesis for prospective evaluation. FAU - Dagli, Pinar Akyuz AU - Dagli PA AUID- ORCID: 0000-0002-4249-8818 AD - Department of Rheumatology, Ankara Bilkent City Hospital, 06800 Ankara, Turkey. FAU - Armagan, Berkan AU - Armagan B AUID- ORCID: 0000-0003-4409-059X AD - Department of Rheumatology, Ankara Bilkent City Hospital, 06800 Ankara, Turkey. FAU - Konak, Hatice Ecem AU - Konak HE AUID- ORCID: 0000-0002-9218-8965 AD - Department of Rheumatology, Ankara Bilkent City Hospital, 06800 Ankara, Turkey. FAU - Bilen Ayhan, Sevgi AU - Bilen Ayhan S AUID- ORCID: 0000-0002-7363-9484 AD - Department of Rheumatology, Ankara Bilkent City Hospital, 06800 Ankara, Turkey. FAU - Ozdemir, Zeynep Ozge AU - Ozdemir ZO AUID- ORCID: 0000-0003-4863-163X AD - Department of Rheumatology, Ankara Bilkent City Hospital, 06800 Ankara, Turkey. FAU - Ozel, Esra Maide AU - Ozel EM AUID- ORCID: 0000-0002-5914-4493 AD - Department of Rheumatology, Ankara Bilkent City Hospital, 06800 Ankara, Turkey. FAU - Buyukaksoy, Muge AU - Buyukaksoy M AUID- ORCID: 0000-0002-4722-5472 AD - Clinic of Internal Medicine, Ankara Bilkent City Hospital, 06800 Ankara, Turkey. FAU - Sozen, Emine Sena AU - Sozen ES AUID- ORCID: 0000-0003-3539-8875 AD - Clinic of Internal Medicine, Ankara Bilkent City Hospital, 06800 Ankara, Turkey. FAU - Atalar, Ebru AU - Atalar E AUID- ORCID: 0000-0003-2708-0373 AD - Department of Rheumatology, Ankara Bilkent City Hospital, 06800 Ankara, Turkey. FAU - Kocak Ulucakoy, Rezan AU - Kocak Ulucakoy R AUID- ORCID: 0000-0001-6206-4841 AD - Department of Rheumatology, Ankara Bilkent City Hospital, 06800 Ankara, Turkey. FAU - Kayacan Erdogan, Esra AU - Kayacan Erdogan E AUID- ORCID: 0000-0002-3570-875X AD - Department of Rheumatology, Ankara Bilkent City Hospital, 06800 Ankara, Turkey. FAU - Guven, Serdar Can AU - Guven SC AUID- ORCID: 0000-0003-1965-9756 AD - Department of Rheumatology, Ankara Bilkent City Hospital, 06800 Ankara, Turkey. FAU - Dogan, Ismail AU - Dogan I AUID- ORCID: 0000-0002-7603-9484 AD - Department of Internal Medicine, Division of Rheumatology, Ankara City Hospital, Ankara Yildirim Beyazit University, 06800 Ankara, Turkey. FAU - Orhan, Kevser AU - Orhan K AUID- ORCID: 0000-0001-8639-751X AD - Department of Rheumatology, Ankara Bilkent City Hospital, 06800 Ankara, Turkey. FAU - Maras, Yuksel AU - Maras Y AUID- ORCID: 0000-0001-9319-0955 AD - Department of Internal Medicine, Division of Rheumatology, Ankara City Hospital, University of Health Sciences, 06800 Ankara, Turkey. FAU - Kucuksahin, Orhan AU - Kucuksahin O AUID- ORCID: 0000-0003-4530-2304 AD - Department of Internal Medicine, Division of Rheumatology, Ankara City Hospital, Ankara Yildirim Beyazit University, 06800 Ankara, Turkey. FAU - Omma, Ahmet AU - Omma A AUID- ORCID: 0000-0003-2582-7445 AD - Department of Internal Medicine, Division of Rheumatology, Ankara City Hospital, University of Health Sciences, 06800 Ankara, Turkey. FAU - Erten, Sukran AU - Erten S AUID- ORCID: 0000-0003-0717-8365 AD - Department of Internal Medicine, Division of Rheumatology, Ankara City Hospital, Ankara Yildirim Beyazit University, 06800 Ankara, Turkey. FAU - Babaoglu, Hakan AU - Babaoglu H AUID- ORCID: 0000-0002-3728-0259 AD - Department of Rheumatology, Ankara Bilkent City Hospital, 06800 Ankara, Turkey. LA - eng PT - Journal Article DEP - 20261008 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - Retrospective Studies MH - *Referral and Consultation/statistics & numerical data/standards MH - *Internal Medicine/statistics & numerical data/methods MH - Female MH - Male MH - *Rheumatic Diseases/diagnosis MH - *Rheumatology/statistics & numerical data/methods MH - Middle Aged MH - Turkey MH - Aged MH - Adult MH - Cohort Studies MH - Logistic Models PMC - PMC13707323 OTO - NOTNLM OT - diagnostic yield OT - health care utilization OT - internal medicine OT - referral and consultation OT - rheumatology COIS- The authors declare that there are no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/08 CRDT- 2026/10/27 10:42 PHST- 2026/08/20 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - medicina62101943 [pii] AID - medicina-62-01943 [pii] AID - 10.3390/medicina62101943 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 8;62(10):1943. doi: 10.3390/medicina62101943. PMID- 42942471 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 8 TI - A Multidisciplinary Care Pathway for Childhood Mouth Breathing: The Pediatric Allergist, ENT, and Orthodontic Perspective. LID - 10.3390/medicina62101940 [doi] LID - 1940 AB - Background: Mouth breathing (MB) in children is a functional alteration characterized by the substitution of nasal breathing with oral or mixed breathing. Persistent MB, particularly when occurring during sleep (nocturnal MB), may be associated with sleep fragmentation, neurobehavioral disturbances, and alterations in craniofacial development. Although established sleep-disordered breathing (SDB), particularly obstructive sleep apnea syndrome (OSAS), is well-recognized and addressed by international guidelines, children presenting with persistent or nocturnal MB without established diagnostic criteria for OSAS may remain outside conventional diagnostic pathways. The multifactorial nature of MB therefore requires the coordinated evaluation of nasal, allergic, upper airway, craniofacial, dental, and orofacial factors. Methods: This narrative review was based primarily on a structured search of the PubMed/MEDLINE database covering publications from database inception to June 2026, supplemented by manual screening of the reference lists of relevant articles and clinical guidelines. The literature was selected according to relevance to the review objectives, methodological robustness, clinical applicability, and contribution to pediatric, allergological, otorhinolaryngological, and dental/orthodontic perspectives. Priority was given to systematic reviews, meta-analyses, randomized controlled trials, clinical practice guidelines, consensus documents, and high-quality observational studies. A total of 79 references were included in the final manuscript. Results: The reviewed evidence indicates that MB may be associated with allergic and nasal conditions, upper airway obstruction, craniofacial and dental abnormalities, and orofacial functional alterations, and may coexist with SDB in selected children. The evidence was synthesized across pediatric, allergological, otorhinolaryngological, and orthodontic perspectives to develop a multidisciplinary care pathway based on the clinical identification of MB, targeted specialist assessment, objective evaluation of SDB when indicated, and individualized management and follow-up. Conclusions: MB may represent an important clinical entry point for the early recognition of potentially treatable conditions and associated SDB. The proposed multidisciplinary care pathway integrates pediatric, allergological, otorhinolaryngological, and dental/orthodontic expertise to support coordinated assessment, individualized management, and longitudinal follow-up of children with MB. FAU - Pecoraro, Luca AU - Pecoraro L AUID- ORCID: 0000-0002-9765-8006 AD - Department of Experimental Medicine Pediatric Section, University of Salento Hospital "Vito Fazzi", 73100 Lecce, Italy. FAU - Attanasi, Martina AU - Attanasi M AD - Pediatric Department, University of Bari Aldo Moro, 70121 Bari, Italy. FAU - Marra, Francesca AU - Marra F AD - ENT Unit, Hospital "Vito Fazzi", 73100 Lecce, Italy. FAU - Romanello, Matteo AU - Romanello M AUID- ORCID: 0009-0009-7960-5267 AD - ENT Unit, Hospital "Vito Fazzi", 73100 Lecce, Italy. FAU - Ferrante, Franco AU - Ferrante F AUID- ORCID: 0000-0001-6392-0048 AD - Dentist, Specialist in Oral Surgery, Private Practitioner, 73100 Lecce, Italy. FAU - Indrio, Flavia AU - Indrio F AD - Department of Experimental Medicine Pediatric Section, University of Salento Hospital "Vito Fazzi", 73100 Lecce, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261008 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - *Mouth Breathing/therapy/diagnosis/physiopathology/complications MH - Child MH - *Pediatrics/methods MH - *Orthodontics/methods MH - *Critical Pathways MH - *Patient Care Team PMC - PMC13707370 OTO - NOTNLM OT - multidisciplinary care pathway OT - nocturnal mouth breathing OT - pediatric mouth breathing OT - primary snoring OT - sleep-disordered breathing COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/08 CRDT- 2026/10/27 10:42 PHST- 2026/09/01 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - medicina62101940 [pii] AID - medicina-62-01940 [pii] AID - 10.3390/medicina62101940 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 8;62(10):1940. doi: 10.3390/medicina62101940. PMID- 42942462 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 7 TI - Comorbidity Count Versus the Charlson Comorbidity Index in Predicting Hospital Stay After Hip Fracture in Older Adults: A Retrospective Cohort Study. LID - 10.3390/medicina62101931 [doi] LID - 1931 AB - Background and Objectives: Hip fracture in older adults is associated with high morbidity, and length of hospital stay is the simplest summary of the acute admission. We aimed to identify the clinical factors associated with prolonged hospital stay in older adults admitted with a hip fracture, and to test whether a weighted comorbidity index predicts stay better than a simple count. Materials and Methods: A retrospective cohort of 624 consecutive patients aged 65 years and older with a trochanteric or femoral neck fracture was studied. Length of stay (LOS) was strongly right-skewed (skewness 5.02) and was log-transformed; comparisons used Mann-Whitney and Kruskal-Wallis tests and Spearman correlations, with multivariable models fitted on 487 operated patients with complete data. Postoperative stay was analyzed as a secondary outcome because the preoperative interval forms part of the total stay. Results: Median stay was 10 days (IQR 8-13) and median wait for surgery was 4 days (IQR 2-6), with only 25.2% operated on within 48 h. In-hospital mortality was 5.3%. The number of comorbidities was the only patient characteristic consistently associated with stay (rho = 0.119, p = 0.003; 1.4% per comorbidity, p < 0.001). The Charlson Comorbidity Index (CCI) showed no association in the unweighted, age-adjusted or severity-graded form. Age, sex and admission hemoglobin effects did not survive non-parametric testing. Conclusions: A simple count of active comorbidities was associated with LOS whereas the CCI was not, and the model containing the count fitted modestly better (adjusted R(2) 0.385 vs. 0.365; index term non-significant). This hypothesis-generating finding suggests that the comorbidity count, which is available on admission, may serve as a pragmatic screening tool for selecting patients for comprehensive geriatric assessment; its use in place of the CCI would require validation in independent cohorts. The preoperative wait remains the dominant modifiable target, although its effect on total stay is largely structural. FAU - Sirbu, Irina AU - Sirbu I AD - Orthopaedic and Traumatology Clinic, "Sf. Spiridon" Clinical Emergency Hospital, 700111 Iasi, Romania. AD - Department of Orthopedics and Traumatology, Faculty of Medicine, "Grigore T. Popa" University of Medicine and Pharmacy, 700115 Iasi, Romania. FAU - Pislaru, Anca Iuliana AU - Pislaru AI AD - Department of Medical Specialties II, Faculty of Medicine, University of Medicine and Pharmacy "Grigore T. Popa", 700115 Iasi, Romania. FAU - Ilie, Adina-Carmen AU - Ilie AC AUID- ORCID: 0000-0002-9152-8534 AD - Department of Medical Specialties II, Faculty of Medicine, University of Medicine and Pharmacy "Grigore T. Popa", 700115 Iasi, Romania. FAU - Stefaniu, Ramona AU - Stefaniu R AD - Department of Medical Specialties II, Faculty of Medicine, University of Medicine and Pharmacy "Grigore T. Popa", 700115 Iasi, Romania. FAU - Albisteanu, Sabinne-Marie AU - Albisteanu SM AD - Department of Medical Specialties II, Faculty of Medicine, University of Medicine and Pharmacy "Grigore T. Popa", 700115 Iasi, Romania. FAU - Turcu, Ana-Maria AU - Turcu AM AD - Department of Medical Specialties II, Faculty of Medicine, University of Medicine and Pharmacy "Grigore T. Popa", 700115 Iasi, Romania. FAU - Veliceasa, Bogdan AU - Veliceasa B AUID- ORCID: 0000-0002-9941-3424 AD - Orthopaedic and Traumatology Clinic, "Sf. Spiridon" Clinical Emergency Hospital, 700111 Iasi, Romania. AD - Department of Orthopedics and Traumatology, Faculty of Medicine, "Grigore T. Popa" University of Medicine and Pharmacy, 700115 Iasi, Romania. FAU - Puha, Bogdan AU - Puha B AUID- ORCID: 0000-0002-9814-7742 AD - Orthopaedic and Traumatology Clinic, "Sf. Spiridon" Clinical Emergency Hospital, 700111 Iasi, Romania. AD - Department of Orthopedics and Traumatology, Faculty of Medicine, "Grigore T. Popa" University of Medicine and Pharmacy, 700115 Iasi, Romania. FAU - Alexa, Ioana Dana AU - Alexa ID AD - Department of Medical Specialties II, Faculty of Medicine, University of Medicine and Pharmacy "Grigore T. Popa", 700115 Iasi, Romania. FAU - Poroh, Manuela AU - Poroh M AD - Department of Biomedical Sciences, Faculty of Medical Bioengineering, "Grigore T. Popa" University of Medicine and Pharmacy of Iasi, 700454 Iasi, Romania. LA - eng GR - This research was funded by The Health Programme (PS) 2021-2027, Policy Objective 4, Priority 3, project title "Support for Enhancing Performance and Innovation in Excellent Medical Doctoral Research", acronym INNODOC-MED, SMIS code 351058./The Health Programme (PS) 2021-2027/ PT - Journal Article DEP - 20261007 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - Retrospective Studies MH - *Length of Stay/statistics & numerical data MH - Female MH - Aged MH - *Hip Fractures/surgery/epidemiology/complications MH - Male MH - *Comorbidity MH - Aged, 80 and over MH - Cohort Studies MH - Proximal Femoral Fractures PMC - PMC13707112 OTO - NOTNLM OT - Charlson comorbidity index OT - comorbidity OT - hip fracture OT - length of stay OT - older adults OT - time to surgery COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/07 CRDT- 2026/10/27 10:42 PHST- 2026/08/30 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - medicina62101931 [pii] AID - medicina-62-01931 [pii] AID - 10.3390/medicina62101931 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 7;62(10):1931. doi: 10.3390/medicina62101931. PMID- 42942461 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 7 TI - Altered Cord Blood Enzyme Activities in Preeclampsia Without Severe Features: A Comparative Observational Study. LID - 10.3390/medicina62101930 [doi] LID - 1930 AB - Background and Objectives: Preeclampsia (PE) is associated with placental dysfunction, endothelial abnormalities, and disturbed redox homeostasis, but enzyme activity patterns in the fetal circulation remain incompletely characterized. This study aimed to compare selected cord blood enzyme activities between pregnancies complicated by PE without severe features and normotensive pregnancies. Materials and Methods: This prospective observational comparative study included 122 participants: 54 women with PE without severe features and 68 women with normotensive pregnancies. All participants delivered vaginally at 37 weeks 1 day to 38 weeks 0 days of gestation. Plasma butyrylcholinesterase (BChE) and erythrocyte acetylcholinesterase (AChE), carbonic anhydrase (CA), glucose-6-phosphate dehydrogenase (G6PD), and glutathione reductase (GR) activities were measured spectrophotometrically. Groups were compared using the two-sided Mann-Whitney U test. Results: Compared with the normotensive pregnancy group, the PE group had lower median AChE (17.60 vs. 26.49 U/g Hb), BChE (1.060 vs. 1.340 EU/mg protein), G6PD (13.79 vs. 15.04 U/g Hb), and GR activities (0.880 vs. 1.075 EU/mg protein), corresponding to reductions of 33.6%, 20.9%, 8.3%, and 18.1%, respectively. Median CA activity was 3.1% higher in the PE group (8.615 vs. 8.360 EU/mg protein). All between-group differences were statistically significant (all p < 0.001). Conclusions: PE without severe features was associated with an enzyme-specific cord blood profile characterized by reduced cholinesterase and redox-related enzyme activities and increased CA activity. These findings support further investigation of fetal enzymatic alterations in PE. FAU - Senturk, Esra AU - Senturk E AUID- ORCID: 0000-0003-4378-7678 AD - Department of Physiology, Faculty of Medicine, Agri Ibrahim Cecen University, 04100 Agri, Turkiye. FAU - Eminov, Elmin AU - Eminov E AUID- ORCID: 0000-0003-1587-1821 AD - Department of Gynecology and Obstetrics, Faculty of Medicine, Duzce University, 81100 Duzce, Turkiye. LA - eng PT - Comparative Study PT - Journal Article PT - Observational Study DEP - 20261007 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - EC 3.1.1.8 (Butyrylcholinesterase) RN - EC 1.1.1.49 (Glucosephosphate Dehydrogenase) RN - EC 3.1.1.7 (Acetylcholinesterase) RN - EC 1.8.1.7 (Glutathione Reductase) RN - EC 4.2.1.1 (Carbonic Anhydrases) SB - IM MH - Humans MH - Female MH - Pregnancy MH - *Pre-Eclampsia/enzymology/blood/physiopathology MH - *Fetal Blood/enzymology MH - Adult MH - Prospective Studies MH - Butyrylcholinesterase/blood/analysis MH - Glucosephosphate Dehydrogenase/blood/analysis MH - Acetylcholinesterase/blood/analysis MH - Glutathione Reductase/blood/analysis MH - Carbonic Anhydrases/blood/analysis MH - Statistics, Nonparametric PMC - PMC13707027 OTO - NOTNLM OT - acetylcholinesterase OT - butyrylcholinesterase OT - carbonic anhydrase OT - glucose-6-phosphate dehydrogenase OT - glutathione reductase OT - oxidative stress OT - preeclampsia OT - umbilical cord blood COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/07 CRDT- 2026/10/27 10:42 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - medicina62101930 [pii] AID - medicina-62-01930 [pii] AID - 10.3390/medicina62101930 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 7;62(10):1930. doi: 10.3390/medicina62101930. PMID- 42942457 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 6 TI - Empirical Antibiotic Initiation for Suspected Early-Onset Neonatal Infection in the Setting of Maternal GBS Screening and Intrapartum Antibiotic Prophylaxis: A Single-Center Retrospective Cohort Study. LID - 10.3390/medicina62101926 [doi] LID - 1926 AB - Background and Objectives: Maternal Group B Streptococcus (GBS) screening and intrapartum antibiotic prophylaxis have reduced early-onset GBS disease. However, suspected early-onset neonatal infection (EONI) remains a broader clinical challenge, as signs prompting empirical antibiotic treatment may also occur in non-infectious disorders of postnatal adaptation. Therefore, this study evaluated the frequency of systemic antibiotic initiation for suspected EONI and the utilization and microbiological yield of blood cultures in a single-center cohort managed with routine maternal GBS screening and intrapartum antibiotic prophylaxis. Materials and Methods: This retrospective cohort study included 2010 mother-newborn dyads delivered between 2019 and 2022. The primary outcome was systemic antibiotic therapy initiated within the first 72 h of life because of suspected EONI. Blood culture utilization and microbiological yield were analyzed separately from the primary treatment outcome. Positive blood culture findings were interpreted descriptively because of the small number of isolates and the possibility of contamination. Results: Empirical systemic antibiotic therapy for suspected EONI was initiated in 71 neonates (3.53%). The observed treatment rates among neonates born to GBS-colonized and non-colonized mothers were 16/402 (3.98%) vs. 55/1608 (3.42%) (RR 1.16, 95% CI 0.67-2.01; p = 0.59). In an exploratory multivariable analysis, the adjusted OR for maternal GBS colonization and antibiotic initiation was 1.33 (95% CI 0.68-2.60; p = 0.408). Lower gestational age was the strongest associated factor. Treated neonates were more frequently preterm and had more respiratory disorders, NICU admissions, pPROM, and lower Apgar scores. Blood cultures were obtained in 79 neonates, including 63 treated for suspected EONI. Nine sampled neonates had positive blood cultures, including seven treated neonates; several isolates were compatible with contamination. No GBS isolates were detected among the sampled neonates. Conclusions: Empirical systemic antibiotics were initiated for suspected EONI in 3.53% of neonates. This treatment frequency does not estimate early-onset sepsis (EOS) incidence. The study did not detect a statistically significant association between maternal GBS colonization and antibiotic initiation, but the wide confidence intervals do not exclude clinically relevant increases in treatment. Selective blood culture sampling, possible contamination, and incomplete microbiological adjudication preclude reliable estimates of culture-confirmed EOS incidence or pathogen distribution. FAU - Zmelonek-Znamirowska, Anna AU - Zmelonek-Znamirowska A AUID- ORCID: 0000-0002-6610-7965 AD - Department of Obstetrics and Gynecology, Voivodeship Combined Hospital, 25-736 Kielce, Poland. FAU - Swiercz, Grzegorz AU - Swiercz G AD - Department of Obstetrics and Gynecology, Voivodeship Combined Hospital, 25-736 Kielce, Poland. AD - Collegium Medicum, Jan Kochanowski University, 25-369 Kielce, Poland. FAU - Mlodawski, Jakub AU - Mlodawski J AUID- ORCID: 0000-0002-5740-9822 AD - Department of Obstetrics and Gynecology, Voivodeship Combined Hospital, 25-736 Kielce, Poland. AD - Collegium Medicum, Jan Kochanowski University, 25-369 Kielce, Poland. FAU - Mlodawska, Marta AU - Mlodawska M AUID- ORCID: 0000-0001-8867-7638 AD - Collegium Medicum, Jan Kochanowski University, 25-369 Kielce, Poland. FAU - Kaczmarek, Piotr AU - Kaczmarek P AUID- ORCID: 0000-0001-8990-2761 AD - Department of Gynecology, Reproductive and Fetal Therapy, Polish Mother's Memorial Hospital Research Institute, 93-338 Lodz, Poland. AD - Endocrinology and Metabolic Diseases Clinic, Medical University of Lodz, 90-419 Lodz, Poland. LA - eng GR - KOPIPOL Association's Creators Fund/ PT - Journal Article DEP - 20261006 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - 0 (Anti-Bacterial Agents) SB - IM MH - Humans MH - Retrospective Studies MH - Female MH - *Antibiotic Prophylaxis/methods/standards/statistics & numerical data MH - Infant, Newborn MH - *Streptococcal Infections/drug therapy/diagnosis/prevention & control MH - *Anti-Bacterial Agents/therapeutic use MH - Pregnancy MH - Cohort Studies MH - Streptococcus agalactiae/drug effects MH - Adult MH - Infectious Disease Transmission, Vertical/prevention & control MH - Pregnancy Complications, Infectious/drug therapy MH - Male PMC - PMC13707366 OTO - NOTNLM OT - Group B Streptococcus OT - blood culture OT - culture-confirmed EOS OT - empirical antibiotic therapy OT - intrapartum antibiotic prophylaxis OT - neonatal infection OT - suspected early-onset neonatal infection COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/06 CRDT- 2026/10/27 10:42 PHST- 2026/08/05 00:00 [received] PHST- 2026/09/13 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/06 00:00 [pmc-release] AID - medicina62101926 [pii] AID - medicina-62-01926 [pii] AID - 10.3390/medicina62101926 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 6;62(10):1926. doi: 10.3390/medicina62101926. PMID- 42942455 OWN - NLM STAT- MEDLINE DCOM- 20261029 LR - 20261029 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 6 TI - Complement Signaling and Regulated Cell Death in Immune-Mediated Glomerular Injury. LID - 10.3390/medicina62101924 [doi] LID - 1924 AB - The complement system is a central component of innate immunity, with essential roles in host defense, immune complex clearance, apoptotic cell removal, and tissue homeostasis. In the kidney, glomerular and tubular structures are continuously exposed to circulating and locally produced complement components. Dysregulated complement activation contributes to a wide spectrum of renal diseases, including membranous nephropathy, IgA nephropathy, ANCA-associated glomerulonephritis, lupus nephritis, thrombotic Imicroangiopathies and selected forms of podocyte injury. Regulated cell death pathways, including apoptosis, necroptosis, pyroptosis, ferroptosis, and autophagy, further shape renal inflammation and injury. These pathways interact bidirectionally with complement: complement effectors such as C3a, C5a, and the membrane attack complex can trigger or modulate inflammatory cell death, whereas dying cells release danger signals and altered-self structures that amplify complement activation. This crosstalk creates self-reinforcing loops of necroinflammation, glomerular injury, tubular damage, and fibrosis. This comprehensive review integrates current evidence on complement biology and regulated cell death in renal disease, emphasizing mechanistic links, disease-specific pathways, diagnostic implications, and emerging therapeutic opportunities. Understanding this interaction may support more precise approaches to complement inhibition, modulation of cell death, and personalized treatment in immune-mediated kidney disease. FAU - Tzali, Eleni AU - Tzali E AUID- ORCID: 0009-0002-1549-8166 AD - Department of Nephrology, School of Medicine, University of Crete, 71003 Heraklion, Greece. AD - Department of Nephrology, Heraklion University Hospital, 71500 Crete, Greece. FAU - Pleros, Christos AU - Pleros C AUID- ORCID: 0000-0001-8123-771X AD - Department of Nephrology, Heraklion University Hospital, 71500 Crete, Greece. FAU - Stylianou, Kostas AU - Stylianou K AUID- ORCID: 0000-0003-3678-9421 AD - Department of Nephrology, School of Medicine, University of Crete, 71003 Heraklion, Greece. AD - Department of Nephrology, Heraklion University Hospital, 71500 Crete, Greece. FAU - Petrakis, Ioannis AU - Petrakis I AUID- ORCID: 0000-0002-0095-9823 AD - Department of Nephrology, School of Medicine, University of Crete, 71003 Heraklion, Greece. AD - Department of Nephrology, Heraklion University Hospital, 71500 Crete, Greece. LA - eng PT - Journal Article PT - Review DEP - 20261006 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - 9007-36-7 (Complement System Proteins) SB - IM MH - *Kidney Diseases/immunology MH - *Kidney Glomerulus/immunology/injuries MH - *Complement System Proteins/immunology MH - Complement Activation/immunology MH - *Regulated Cell Death/immunology MH - Humans MH - Animals MH - Immunity, Innate PMC - PMC13707358 OTO - NOTNLM OT - complement system OT - glomerular diseases OT - regulated cell death COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/06 CRDT- 2026/10/27 10:42 PHST- 2026/07/13 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/06 00:00 [pmc-release] AID - medicina62101924 [pii] AID - medicina-62-01924 [pii] AID - 10.3390/medicina62101924 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 6;62(10):1924. doi: 10.3390/medicina62101924. PMID- 42942453 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 6 TI - Association of Baseline Serum DKK-1 Levels with Subsequent Bone Metastasis in Stage III Breast Cancer. LID - 10.3390/medicina62101922 [doi] LID - 1922 AB - Background and Objectives: Bone metastasis is the most frequent site of distant spread in breast cancer, yet reliable biomarkers capable of identifying patients at increased risk before metastatic disease becomes clinically evident remain limited. We investigated whether baseline serum Dickkopf-1 (DKK-1) and irisin levels were associated with subsequent bone and liver metastasis in patients with stage III breast cancer. Materials and Methods: Sixty-three patients with stage III breast cancer were retrospectively included. DKK-1 and irisin concentrations were measured using enzyme-linked immunosorbent assay in archived serum samples collected at the time of initial diagnosis, before the start of definitive treatment. Associations between biomarker levels and subsequent metastatic outcomes were evaluated using maximally selected rank statistics for cut-off determination, Kaplan-Meier survival analysis, restricted cubic spline analysis, and Cox proportional hazards regression models. Results: During follow-up, 29 patients developed bone metastasis. Baseline serum DKK-1 levels were significantly higher in patients who subsequently developed bone metastasis than in those who remained metastasis-free (p = 0.005), whereas irisin levels showed no significant difference. An exploratory optimal DKK-1 cut-off value of 66.75 pg/mL was identified. High baseline DKK-1 remained independently associated with bone metastasis-free survival after multivariable Cox proportional hazards regression including T stage, histological grade, Ki-67 index, lymphovascular invasion, irisin, ALP and CA15-3 (HR = 7.48, 95% CI 1.67-33.43, p = 0.008). Elevated baseline ALP was also independently associated with bone metastasis-free survival (BMFS) (HR = 4.12, 95% CI 1.35-12.53, p = 0.013). Neither DKK-1 nor irisin was significantly associated with subsequent liver metastasis. Conclusions: Elevated baseline serum DKK-1, but not irisin, was independently associated with shorter BMFS in patients with stage III breast cancer. Baseline DKK-1 may represent a candidate biomarker for identifying patients at increased risk of skeletal metastasis; however, these exploratory findings require external validation in prospective multicenter studies before any clinical implementation can be considered. FAU - Gul, Gurkan AU - Gul G AUID- ORCID: 0000-0001-8474-6763 AD - Department of Medical Oncology, Elazig Fethi Sekin City Hospital, 23280 Elazig, Turkiye. FAU - Gunenc, Damla AU - Gunenc D AUID- ORCID: 0000-0002-7592-2021 AD - Department of Medical Oncology, Izmir City Hospital, 35540 Izmir, Turkiye. FAU - Kutlu, Ozlem AU - Kutlu O AUID- ORCID: 0000-0001-5050-3333 AD - Department of Medical Oncology, Izmir City Hospital, 35540 Izmir, Turkiye. FAU - Ulas, Mustafa AU - Ulas M AD - Department of Physiology, Faculty of Medicine, Samsun University, 55080 Samsun, Turkiye. FAU - Aksoy, Aziz AU - Aksoy A AD - Department of Bioengineering, Malatya Turgut Ozal University, 44210 Malatya, Turkiye. FAU - Esenkaya, Erdinc AU - Esenkaya E AUID- ORCID: 0009-0000-1715-0230 AD - Department of Medical Oncology, Izmir City Hospital, 35540 Izmir, Turkiye. FAU - Aksoy, Asude AU - Aksoy A AD - Department of Medical Oncology, Elazig Fethi Sekin City Hospital, 23280 Elazig, Turkiye. LA - eng PT - Journal Article DEP - 20261006 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - 0 (Intercellular Signaling Peptides and Proteins) RN - 0 (DKK1 protein, human) RN - 0 (Fibronectins) RN - 0 (Biomarkers, Tumor) RN - 0 (FNDC5 protein, human) SB - IM MH - Humans MH - Female MH - *Intercellular Signaling Peptides and Proteins/blood/analysis MH - *Bone Neoplasms/secondary/blood MH - *Breast Neoplasms/blood/pathology MH - Middle Aged MH - Fibronectins/blood/analysis MH - Retrospective Studies MH - Adult MH - Biomarkers, Tumor/blood MH - Neoplasm Staging MH - Aged MH - Proportional Hazards Models MH - Kaplan-Meier Estimate MH - Enzyme-Linked Immunosorbent Assay PMC - PMC13706943 OTO - NOTNLM OT - DKK-1 OT - ELISA OT - bone metastasis OT - breast cancer OT - irisin OT - prognosis OT - serum biomarker COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/06 CRDT- 2026/10/27 10:42 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/06 00:00 [pmc-release] AID - medicina62101922 [pii] AID - medicina-62-01922 [pii] AID - 10.3390/medicina62101922 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 6;62(10):1922. doi: 10.3390/medicina62101922. PMID- 42942449 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 5 TI - Blood Product Transfusion Patterns in One Lithuanian and Two Polish Academic Emergency Departments: A Retrospective Comparative Study. LID - 10.3390/medicina62101918 [doi] LID - 1918 AB - Background and Objectives: Blood product transfusion is an important emergency intervention for patients with acute bleeding, severe symptomatic anemia, and selected hematological conditions. Patterns of ED transfusion may differ according to the local case mix, referral pathways, and blood bank processes. We compared transfusion-related characteristics and outcomes among patients treated in one Lithuanian and two Polish academic emergency departments. Materials and Methods: We performed a retrospective comparative study of adults (aged >/=18 years) in whom a blood product transfusion was initiated in the ED between 1 January 2022 and 31 December 2024 at one Lithuanian and two Polish academic hospitals. We compared patient characteristics, indication categories, shock index values, time to transfusion, blood product use, adjunctive therapies, laboratory values, ED disposition, hospital length of stay, and in-hospital mortality. Findings are reported as center-cohort comparisons and are not intended to represent national practice. Results: A total of 670 patients were included: 367 at the Lithuanian center and 303 across the two Polish centers. GI bleeding was significantly more common in the Lithuanian center cohort (48.8% vs. 22.8%; OR 3.22 [95% CI 2.30-4.53]; p < 0.001), whereas anemia and malignancy-related anemia were more frequent in the Polish center cohort (anemia: 34.7% vs. 18.3%, OR 0.42 [95% CI 0.29-0.6]; p < 0.001; cancer: 26.4% vs. 7.6%, OR 0.23 [95% CI 0.15-0.37]; p < 0.001). The median shock index was significantly higher in the Lithuanian center cohort than in the Polish center cohort (0.9; [IQR 0.7-1.2] vs. 0.7; IQR 0.6-0.9; 0.2 [95% CI 0.1-0.3]; p < 0.001), and pre-transfusion hemoglobin was lower (64.5 g/L [IQR 50-86] vs. 119 g/L [IQR 83-139]; p < 0.001). Uncrossmatched RBC units were more frequently administered in the Lithuanian center cohort (133 (36.9%) vs. 47 (15.6%); OR 3.18 [95% CI 2.18-4.64]; p < 0.001), as was fresh frozen plasma (71 (19.5%) vs. 3 (1%); OR 24.23 [95% CI 7.55-77.8]; p < 0.001). Conversely, platelet transfusions were more common in the Polish center cohort (17 (4.6%) vs. 45 (14.9%), OR 0.28 [95% CI 0.16-0.5]; p < 0.001). The median time from ED arrival to transfusion was longer in the Lithuanian center cohort than in the Polish center cohort (150 [IQR 75-256] vs. 117 [IQR 103-154], 23 [95% CI 9-39]; p = 0.002). Hospital admission was significantly more frequent in the Lithuanian cohort than in the Polish cohort (350 (95.4%) vs. 112 (37.0%); p < 0.001). Among admitted patients, the median hospital length of stay was significantly longer in the Lithuanian cohort compared with the Polish cohort (6 days [IQR 3-11] vs. 1 day [IQR 1-5]; 95% CI for the difference 3-4; p < 0.001). In-hospital mortality was 33.5% (n = 123) in the Lithuanian cohort and 13.9% (n = 42) in the Polish cohort (OR 3.13 [95% CI 2.12-4.63]; p < 0.001). Conclusions: Among transfused patients in the three participating academic EDs, the case mix, blood product use, transfusion timing, and unadjusted outcomes differed substantially. These center-specific findings may inform future prospective studies of how referral pathways, clinical populations, and local transfusion processes influence ED blood product utilization. They do not establish differences in national practice, transfusion appropriateness, or causal effects on clinical outcomes. FAU - Damuleviciute, Gaile AU - Damuleviciute G AD - Emergency Medicine Department, Lithuanian University of Health Sciences, 44307 Kaunas, Lithuania. FAU - Blaziene, Kristina AU - Blaziene K AD - Emergency Medicine Department, Lithuanian University of Health Sciences, 44307 Kaunas, Lithuania. FAU - Nozewski, Jakub AU - Nozewski J AUID- ORCID: 0000-0002-8765-233X AD - Emergency Department, Collegium Medicum, Nicolaus Copernicus University in Torun, Jagiellonska 13-15, 85-067 Bydgoszcz, Poland. FAU - Miskinaite, Greta AU - Miskinaite G AUID- ORCID: 0009-0008-9559-9234 AD - Lithuanian University of Health Sciences, 44307 Kaunas, Lithuania. FAU - Monstaviciue, Goda AU - Monstaviciue G AD - Lithuanian University of Health Sciences, 44307 Kaunas, Lithuania. FAU - Janiszewska, Kinga AU - Janiszewska K AD - Department of General and Minimally Invasive Surgery, Jan Biziel University Hospital No. 2, 85-168 Bydgoszcz, Poland. FAU - Klosiewicz, Tomasz AU - Klosiewicz T AUID- ORCID: 0000-0003-0970-4664 AD - Department of Medical Rescue, Poznan University of Medical Sciences, 7 Rokietnicka Street, 60-806 Poznan, Poland. FAU - Woszczyk, Dawid AU - Woszczyk D AUID- ORCID: 0000-0002-2759-4625 AD - Doctoral School, Poznan University of Medical Sciences, 10 Fredry Street, 61-701 Poznan, Poland. FAU - Konieczka, Patryk AU - Konieczka P AUID- ORCID: 0000-0002-8187-7087 AD - Department of Emergency Medicine, Poznan University of Medical Sciences, 7 Rokietnicka Street, 60-806 Poznan, Poland. FAU - Remeikiene, Diana AU - Remeikiene D AD - Department of Oncology and Hematology, Institute of Oncology, Lithuanian University of Health Sciences, Eiveniu Str. 2, 50009 Kaunas, Lithuania. FAU - Aukstakalnis, Vytautas AU - Aukstakalnis V AD - Emergency Medicine Department, Lithuanian University of Health Sciences, 44307 Kaunas, Lithuania. LA - eng PT - Comparative Study PT - Journal Article DEP - 20261005 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - Retrospective Studies MH - Lithuania MH - Female MH - Male MH - Poland MH - Middle Aged MH - Aged MH - *Emergency Service, Hospital/statistics & numerical data/organization & administration MH - Adult MH - *Blood Transfusion/statistics & numerical data/methods MH - Length of Stay/statistics & numerical data MH - *Blood Component Transfusion/statistics & numerical data/methods MH - Anemia/therapy MH - Hospital Mortality PMC - PMC13707164 OTO - NOTNLM OT - blood product management OT - blood transfusion OT - emergency department OT - patient blood management OT - restrictive transfusion strategy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/05 CRDT- 2026/10/27 10:42 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/05 00:00 [pmc-release] AID - medicina62101918 [pii] AID - medicina-62-01918 [pii] AID - 10.3390/medicina62101918 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 5;62(10):1918. doi: 10.3390/medicina62101918. PMID- 42942447 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 4 TI - Do Gastric Balloons Have a Future? Intragastric Balloons in the Era of Highly Effective Obesity Pharmacotherapy. LID - 10.3390/medicina62101916 [doi] LID - 1916 AB - Intragastric balloons (IGBs) were developed to bridge the gap between lifestyle intervention and Metabolic and Bariatric Surgery (MBS), but the advent of highly efficacious pharmacotherapies and Endoscopic Sleeve Gastroplasty (ESG) has challenged their role. This narrative review synthesises contemporary evidence and guideline recommendations regarding IGBs, focusing on short-term efficacy, durability, safety, psychological aspects, and health system considerations in comparison with Glucagon-Like Peptide-1 Receptor Agonists (GLP-1 RAs), dual GIP (Glucose-dependent Insulinotropic Polypeptide)/GLP-1 RAs, ESG, and MBS. Across randomised trials and meta-analyses, IGBs consistently produce modest short-term weight loss of approximately 10-15% total body weight at six months, with frequent weight regain following removal and limited long-term benefit. In contrast, modern pharmacotherapy achieves greater and more durable weight loss with fewer procedural risks. Current guidelines therefore position IGBs as adjunctive or bridging interventions reserved for selected patient subgroups, rather than as a dominant intermediate therapy. Overall, current evidence supports a selective role for IGBs as bridging, adjunctive, or combination interventions within multimodal obesity pathways, particularly for patients who cannot access or tolerate pharmacotherapy or who require a behavioural and metabolic bridge to surgery. FAU - Sibal, Rhea AU - Sibal R AD - Faculty of Life Sciences and Medicine, King's College London, London SE1 1UL, UK. FAU - Balamurugan, G AU - Balamurugan G AUID- ORCID: 0000-0001-6028-0444 AD - Department of General Surgery, South Tyneside and Sunderland NHS Foundation Trust, Sunderland SR4 7TP, UK. FAU - Aradaib, Mohammed AU - Aradaib M AUID- ORCID: 0000-0002-9656-610X AD - Department of General Surgery, South Tyneside and Sunderland NHS Foundation Trust, Sunderland SR4 7TP, UK. FAU - Graham, Yitka AU - Graham Y AD - Department of General Surgery, South Tyneside and Sunderland NHS Foundation Trust, Sunderland SR4 7TP, UK. AD - Helen McArdle Nursing and Care Research Institute, University of Sunderland, Sunderland SR6 0DD, UK. AD - Faculty of Biomedical Sciences, Austral University, Buenos Aires B1406, Argentina. AD - Faculty of Psychology, University of Anahuac, Mexico City 52786, Mexico. FAU - Mahawar, Kamal AU - Mahawar K AD - Department of General Surgery, South Tyneside and Sunderland NHS Foundation Trust, Sunderland SR4 7TP, UK. AD - Helen McArdle Nursing and Care Research Institute, University of Sunderland, Sunderland SR6 0DD, UK. LA - eng PT - Journal Article PT - Review DEP - 20261004 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - 0 (Anti-Obesity Agents) SB - IM MH - Humans MH - *Gastric Balloon/trends/standards MH - *Obesity/drug therapy/therapy/surgery MH - Weight Loss MH - Bariatric Surgery/methods MH - Anti-Obesity Agents/therapeutic use PMC - PMC13707365 OTO - NOTNLM OT - endoscopic bariatric therapy OT - incretin therapy OT - intragastric balloon OT - metabolic surgery OT - obesity OT - pharmacotherapy OT - weight loss COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/04 CRDT- 2026/10/27 10:42 PHST- 2026/07/22 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/04 00:00 [pmc-release] AID - medicina62101916 [pii] AID - medicina-62-01916 [pii] AID - 10.3390/medicina62101916 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 4;62(10):1916. doi: 10.3390/medicina62101916. PMID- 42942435 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 1 TI - Effects of Piracetam and Hyperbaric Oxygen on Histopathological and Circulating Biochemical Responses Following Sciatic Nerve Crush Injury in Rats. LID - 10.3390/medicina62101904 [doi] LID - 1904 AB - Background and Objectives: Peripheral nerve injury initiates a series of degenerative, inflammatory, and reparative responses. Piracetam and hyperbaric oxygen (HBO) have been proposed as potential therapeutic interventions; however, their effects during the early phase following sciatic nerve injury require further investigation. This study evaluated early histopathological and circulating biochemical responses to piracetam and HBO in a rat model of sciatic nerve injury. Materials and Methods: Forty male Wistar Albino rats were randomly allocated to four groups following experimental sciatic nerve injury: an untreated injury control group (Group K), a hyperbaric oxygen therapy group (Group HBO), an intraperitoneal piracetam group (Group P), and a combined hyperbaric oxygen plus piracetam group (Group HBO + P). After 14 days, the rats were euthanised, and sciatic nerve tissues and blood samples were collected for histopathological and biochemical analyses. Results: No statistically significant differences were observed among the four groups with respect to axonal continuity, capillary formation, or myelin sheath regeneration. Fibroblast density was increased in Group P compared with Group K (p = 0.025), while no significant differences were detected among the remaining groups. Inflammatory cell counts were higher in Group P than in Group K (p = 0.037) and elevated in Group HBO + P compared with Group K (p = 0.014). Hypoxia-inducible factor-1 alpha (HIF-1alpha) expression was highest in Group K. Additionally, neuron-specific enolase levels were significantly increased in Group P compared with Group K (p = 0.006). Conclusions: Under the conditions of this 14-day experimental study, piracetam and HBO were associated with differences in selected circulating biochemical parameters but did not produce statistically significant improvements in the assessed histopathological indices. The findings were interpreted as early responses to treatment, and longer follow-up studies are needed to determine whether these changes translate into sustained axonal regeneration or functional recovery. FAU - Canoglu, Umut Caner AU - Canoglu UC AD - Anesthesiology and Reanimation Department, Istanbul Okan University, Istanbul 34959, Turkey. FAU - Yorulmaz, Ilknur Suidiye AU - Yorulmaz IS AUID- ORCID: 0000-0002-1441-6360 AD - Anesthesiology and Reanimation Department, Duzce University, Duzce 81620, Turkey. FAU - Gamsizkan, Mehmet AU - Gamsizkan M AD - Pathology Department, Duzce University, Duzce 81620, Turkey. FAU - Iskender, Abdulkadir AU - Iskender A AD - Anesthesiology and Reanimation Department, Medical Science University, Bursa 16000, Turkey. FAU - Alpay, Merve AU - Alpay M AUID- ORCID: 0000-0002-8782-9561 AD - Biochemistry Department, Duzce University, Duzce 81620, Turkey. FAU - Sungur, Mehmet Ali AU - Sungur MA AUID- ORCID: 0000-0001-5380-0819 AD - Biostatistics Department, Duzce University, Duzce 81620, Turkey. FAU - Sheikhvatan, Mehrdad AU - Sheikhvatan M AUID- ORCID: 0000-0002-5957-5277 AD - Department of Medical Biology and Genetics, Istanbul Okan University, Istanbul 34959, Turkey. LA - eng PT - Journal Article DEP - 20261001 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - ZH516LNZ10 (Piracetam) SB - IM MH - Animals MH - *Hyperbaric Oxygenation/methods/standards MH - *Piracetam/therapeutic use/pharmacology MH - Rats MH - Male MH - Rats, Wistar MH - *Sciatic Nerve/injuries MH - Disease Models, Animal MH - Random Allocation MH - *Crush Injuries/drug therapy MH - Nerve Regeneration/drug effects MH - Diving and Hyperbaric Medicine/methods MH - *Peripheral Nerve Injuries/therapy PMC - PMC13706824 OTO - NOTNLM OT - animal model OT - hyperbaric oxygen OT - nerve injury OT - piracetam OT - regeneration COIS- All authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/01 CRDT- 2026/10/27 10:42 PHST- 2026/08/13 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - medicina62101904 [pii] AID - medicina-62-01904 [pii] AID - 10.3390/medicina62101904 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 1;62(10):1904. doi: 10.3390/medicina62101904. PMID- 42942433 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 1 TI - Muscle-Preserving Versus Conventional Midline Expansive Open-Door Laminoplasty for Multilevel Cervical Spondylotic Myelopathy: A 10-Year Comparative Cohort Study. LID - 10.3390/medicina62101902 [doi] LID - 1902 AB - Background and Objectives: Conventional midline expansive open-door laminoplasty (EODL) may disrupt the posterior cervical extensor mechanism. This study compared 10-year outcomes after conventional midline and unilateral para-midline muscle-preserving EODL. Materials and Methods: This retrospective cohort included 100 adults who underwent EODL for multilevel cervical spondylotic myelopathy from 1 January 2011 through 31 December 2015 (42 conventional and 58 muscle-preserving). At 10 years, 94 patients completed clinical assessments and 53 underwent MRI examinations. The primary outcome was the postoperative trajectory of the 17-point Japanese Orthopaedic Association (JOA) score from 3-6 months through 10 years. Models adjusted for age, sex, baseline outcome, and decompressed levels; sensitivity models additionally adjusted for operative year. Results: After operative-year adjustment, group-by-time interactions were significant for JOA (F(4, 385.749) = 9.094, p < 0.001), visual analog scale (VAS; F(4, 384.457) = 5.559, p < 0.001), and Neck Disability Index (NDI; F(4, 396.021) = 5.109, p = 0.001). At 10 years, adjusted means in the muscle-preserving versus conventional groups were 10.121 vs. 8.738 for JOA (difference, 1.383; 95% CI, 0.867-1.899), 3.718 vs. 5.946 for VAS (difference, -2.228; 95% CI, -2.643 to -1.813), and 15.395 vs. 20.592 for NDI (difference, -5.197; 95% CI, -6.952 to -3.442; all p < 0.001). Observed 10-year lordosis was 4.73 degrees vs. -3.04 degrees , sagittal vertical axis was 18.83 vs. 38.22 mm, and range of motion was 14.55 degrees vs. 8.57 degrees (all p < 0.001). Adjusted normalized paraspinal cross-sectional area was 7.840 vs. 5.591 (difference, 2.250; 95% CI, 1.752-2.748; p < 0.001). Operative time, blood loss, and hospital stay were lower with the muscle-preserving approach (88.89 vs. 121.82 min, 169.48 vs. 233.33 mL, and 6.12 vs. 7.19 days; all p < 0.001). Reoperation occurred in 4/58 vs. 6/42 patients (p = 0.314); the study was not powered to establish safety equivalence. Conclusions: The muscle-preserving approach was associated with more favorable intermediate- and long-term clinical, radiographic, and muscle-morphology outcomes. These associations persisted after operative-year adjustment for the clinical and CSA outcomes but do not establish causality because residual temporal and treatment-selection confounding remain possible. FAU - Chen, Hao-Wen AU - Chen HW AD - Department of Orthopedics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970473, Taiwan. AD - School of Medicine, Tzu Chi University, Hualien 970374, Taiwan. FAU - Yeh, Kuang-Ting AU - Yeh KT AUID- ORCID: 0000-0002-6154-2167 AD - Department of Orthopedics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970473, Taiwan. AD - School of Medicine, Tzu Chi University, Hualien 970374, Taiwan. AD - Institute of Medical Sciences, Tzu Chi University, Hualien 970374, Taiwan. AD - Graduate Institute of Clinical Pharmacy, Tzu Chi University, Hualien 970374, Taiwan. AD - Department of Medical Education, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970473, Taiwan. FAU - Wu, Wen-Tien AU - Wu WT AUID- ORCID: 0000-0001-6945-712X AD - Department of Orthopedics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970473, Taiwan. AD - School of Medicine, Tzu Chi University, Hualien 970374, Taiwan. AD - Institute of Medical Sciences, Tzu Chi University, Hualien 970374, Taiwan. LA - eng GR - TCRD 113-060/Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation/ PT - Comparative Study PT - Journal Article DEP - 20261001 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - Male MH - Female MH - *Laminoplasty/methods/standards MH - Middle Aged MH - Retrospective Studies MH - *Spondylosis/surgery MH - Aged MH - Cohort Studies MH - *Cervical Vertebrae/surgery MH - Treatment Outcome MH - Magnetic Resonance Imaging/methods MH - *Spinal Cord Diseases/surgery PMC - PMC13706684 OTO - NOTNLM OT - cervical vertebrae OT - follow-up studies OT - laminoplasty OT - spinal cord compression OT - spondylosis OT - treatment outcome COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/01 CRDT- 2026/10/27 10:42 PHST- 2026/07/17 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - medicina62101902 [pii] AID - medicina-62-01902 [pii] AID - 10.3390/medicina62101902 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 1;62(10):1902. doi: 10.3390/medicina62101902. PMID- 42942432 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 1 TI - Peri-Interventional Prophylactic Plasma Transfusion. LID - 10.3390/medicina62101901 [doi] LID - 1901 AB - Human plasma remains one of the most frequently transfused blood products despite increasing data that many transfusions are unnecessary and unsupported by clinical evidence. This review summarises current evidence regarding the haemostatic properties of therapeutic plasma, their effects on conventional coagulation tests, and contemporary use in non-bleeding patients undergoing surgery or invasive procedures, as well as for anticoagulant reversal. Conventional coagulation tests, particularly the international normalised ratio (INR), correlate poorly with bleeding risk and are only minimally corrected by commonly administered plasma doses. Current evidence demonstrates that prophylactic plasma transfusion does not reduce peri-procedural bleeding in most patients while exposing them to transfusion-related complications which can lead to worse outcomes. Recent international guidelines consistently discourage routine plasma transfusion solely to correct mildly abnormal coagulation tests, and recommend four-factor prothrombin complex concentrate rather than plasma for urgent vitamin K antagonist reversal. FAU - Filipescu, Daniela AU - Filipescu D AUID- ORCID: 0000-0003-2144-464X AD - Emergency Institute of Cardiovascular Disease "Prof. Dr. CC Iliescu", 022322 Bucharest, Romania. AD - Faculty of Medicine, University of Medicine and Pharmacy "Carol Davila", 020021 Bucharest, Romania. FAU - Predoi, Cornelia-Elena AU - Predoi CE AD - Emergency Institute of Cardiovascular Disease "Prof. Dr. CC Iliescu", 022322 Bucharest, Romania. AD - Faculty of Medicine, University of Medicine and Pharmacy "Carol Davila", 020021 Bucharest, Romania. FAU - Balan, Cosmin AU - Balan C AUID- ORCID: 0000-0002-1477-6390 AD - Emergency Institute of Cardiovascular Disease "Prof. Dr. CC Iliescu", 022322 Bucharest, Romania. AD - Faculty of Medicine, University of Medicine and Pharmacy "Carol Davila", 020021 Bucharest, Romania. FAU - Filipescu, Radu AU - Filipescu R AD - Emergency Institute of Cardiovascular Disease "Prof. Dr. CC Iliescu", 022322 Bucharest, Romania. AD - Faculty of Medicine, University of Medicine and Pharmacy "Carol Davila", 020021 Bucharest, Romania. FAU - Guz, Dragos AU - Guz D AUID- ORCID: 0009-0009-8593-2598 AD - Emergency Institute of Cardiovascular Disease "Prof. Dr. CC Iliescu", 022322 Bucharest, Romania. AD - Faculty of Medicine, University of Medicine and Pharmacy "Carol Davila", 020021 Bucharest, Romania. FAU - Margineanu, Cornelia AU - Margineanu C AUID- ORCID: 0009-0007-2751-7391 AD - Faculty of Medicine, University of Medicine and Pharmacy "Carol Davila", 020021 Bucharest, Romania. FAU - Robu, Cornel AU - Robu C AD - Emergency Institute of Cardiovascular Disease "Prof. Dr. CC Iliescu", 022322 Bucharest, Romania. AD - Faculty of Medicine, University of Medicine and Pharmacy "Carol Davila", 020021 Bucharest, Romania. FAU - Bubenek-Turconi, Serban-Ion AU - Bubenek-Turconi SI AD - Emergency Institute of Cardiovascular Disease "Prof. Dr. CC Iliescu", 022322 Bucharest, Romania. AD - Faculty of Medicine, University of Medicine and Pharmacy "Carol Davila", 020021 Bucharest, Romania. FAU - Stefan, Mihai Gabriel AU - Stefan MG AD - Emergency Institute of Cardiovascular Disease "Prof. Dr. CC Iliescu", 022322 Bucharest, Romania. AD - Faculty of Medicine, University of Medicine and Pharmacy "Carol Davila", 020021 Bucharest, Romania. LA - eng PT - Journal Article PT - Review DEP - 20261001 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - 0 (Anticoagulants) SB - IM MH - Humans MH - *Plasma MH - *Blood Component Transfusion/methods/standards MH - International Normalized Ratio MH - Anticoagulants/therapeutic use MH - Blood Coagulation Tests/methods MH - Hemorrhage/prevention & control PMC - PMC13707068 OTO - NOTNLM OT - anticoagulant reversal OT - coagulation tests OT - fresh frozen plasma OT - international normalised ratio OT - invasive procedures OT - patient blood management OT - perioperative bleeding OT - plasma transfusion COIS- DF reported serving as principal investigator for Octapharma, receiving grants from CSL Vifor, personal fees from GPATI Foundation, and nonfinancial support from Foundation for Health, Patient Safety, and Patient Blood Management (PBM) outside the submitted work and serving as co-chair of the Romanian PBM working group, the World Federation Society of Anaesthesiologists perioperative PBM working group, and expert member of Blood and Beyond. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/01 CRDT- 2026/10/27 10:42 PHST- 2026/07/23 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - medicina62101901 [pii] AID - medicina-62-01901 [pii] AID - 10.3390/medicina62101901 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 1;62(10):1901. doi: 10.3390/medicina62101901. PMID- 42942430 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Oct 1 TI - Fertility Preservation in Women with Endometriosis: Indications, Timing, Techniques, and Reproductive Outcomes. LID - 10.3390/medicina62101899 [doi] LID - 1899 AB - Endometriosis is a chronic inflammatory disease frequently associated with infertility. Reproductive potential may be impaired by ovarian endometriomas, age-related decline, inflammatory and anatomical mechanisms, and ovarian surgery. This comprehensive narrative review summarises current evidence on fertility-preservation strategies in women with endometriosis, based on a structured literature search updated to 9 August 2026. Oocyte vitrification is the most frequently used and best-studied technique, while embryo cryopreservation may be considered according to individual circumstances, and ovarian tissue cryopreservation currently has a limited disease-specific role. Observational studies consistently show that increasing age and previous ovarian surgery are associated with fewer retrieved and mature oocytes, supporting counselling before ovarian surgery whenever feasible. Recent phenotype-stratified data further suggest that ovarian endometriosis is more consistently associated with reduced ovarian response than superficial or deep disease without ovarian involvement. Gonadotropin-releasing hormone antagonist and progestin-primed stimulation protocols both appear feasible, and repeated cycles may increase cumulative oocyte yield. Vitrified oocytes can survive warming and result in live birth; however, reproductive outcomes depend strongly on age and the number of oocytes available. Coexisting adenomyosis illustrates a limitation of gamete preservation because uterine factors may continue to affect implantation, miscarriage and later pregnancy outcomes. Recent follow-up data demonstrate that many women conceive spontaneously and that only a proportion return to use cryopreserved material. Consequently, the incremental benefit of preventive oocyte cryopreservation over expectant management and later fertility treatment remains unknown. Current evidence does not support systematic fertility preservation for all women with endometriosis. Early counselling and selective treatment should be considered, particularly in women with bilateral or recurrent ovarian disease, previous or planned ovarian surgery, and delayed reproductive plans. FAU - Tramontano, Luca AU - Tramontano L AUID- ORCID: 0000-0002-8654-3123 AD - WENGER Center for Gynecology, Endometriosis and Fertility, Hopital de La Tour, Rue de Veyrot 39, 1217 Meyrin, Switzerland. FAU - Wenger, Jean-Marie AU - Wenger JM AD - WENGER Center for Gynecology, Endometriosis and Fertility, Hopital de La Tour, Rue de Veyrot 39, 1217 Meyrin, Switzerland. FAU - Marci, Roberto AU - Marci R AUID- ORCID: 0000-0002-9230-6685 AD - Department of Translational Medicine and for Romagna, University of Ferrara, Via Luigi Borsari 46, 44121 Ferrara, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261001 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - Female MH - *Endometriosis/complications/physiopathology MH - *Fertility Preservation/methods/standards MH - Cryopreservation/methods MH - Pregnancy MH - Oocytes MH - Pregnancy Outcome MH - Infertility, Female/etiology PMC - PMC13706754 OTO - NOTNLM OT - endometriosis OT - fertility preservation OT - live birth OT - oocyte cryopreservation OT - oocyte vitrification OT - ovarian endometrioma OT - ovarian reserve OT - ovarian surgery COIS- L.T. serves as a Guest Editor of the Special Issue "Reproductive Medicine in Clinical Practice" and will not participate in the editorial handling, peer-review process, or decision-making for this manuscript. The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/01 CRDT- 2026/10/27 10:42 PHST- 2026/08/09 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - medicina62101899 [pii] AID - medicina-62-01899 [pii] AID - 10.3390/medicina62101899 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Oct 1;62(10):1899. doi: 10.3390/medicina62101899. PMID- 42942429 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Sep 30 TI - Accelerated Atherosclerosis. LID - 10.3390/medicina62101898 [doi] LID - 1898 AB - Accelerated atherosclerosis (AA) denotes a disproportionately early and rapid progression of arterial atheroma unpredictable by standard tools. Clinicians often rely on simplified frameworks for cardiovascular risk assessment, which may overlook genetic and pathological conditions associated with AA. Some of these are well established, others are considered future therapy targets. This review examines the conditions in which AA is most firmly established, including rheumatoid arthritis (RA), systemic lupus erythematosus (SLE), antiphospholipid syndrome, chronic kidney disease (CKD), human immunodeficiency virus (HIV), diabetes mellitus, and psoriasis. We also examined the importance of genetic determinants of AA, with particular emphasis on clonal hematopoiesis of indeterminate potential (CHIP) and lipoprotein (a) (Lp(a)); contemporary imaging-based detection and monitoring; the diagnostic role of circulating non-coding microRNAs; and complementary protein biomarkers of residual inflammatory risk. After identifying biomarkers of increased cardiovascular risk and possible AA, clinicians should assess atherosclerotic disease directly using the available imaging techniques, most of which are non-invasive. The clinical consequences of AA are discussed and a comprehensive review of the therapeutic options is provided to offer a practical approach. FAU - Nechita, Alexandru-Cristian AU - Nechita AC AD - Department of Cardiology, Clinical Emergency Hospital Sfantul Pantelimon, 021659 Bucharest, Romania. AD - Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, 020021 Bucharest, Romania. FAU - Popescu, Dan-Cristian AU - Popescu DC AD - Department of Cardiology, Clinical Emergency Hospital Sfantul Pantelimon, 021659 Bucharest, Romania. AD - Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, 020021 Bucharest, Romania. AD - Faculty of Medicine, Transilvania University, 500036 Brasov, Romania. FAU - Diaconu, Mara AU - Diaconu M AUID- ORCID: 0009-0000-5167-6738 AD - Department of Cardiology, Clinical Emergency Hospital Sfantul Pantelimon, 021659 Bucharest, Romania. AD - Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, 020021 Bucharest, Romania. AD - Faculty of Medicine, Transilvania University, 500036 Brasov, Romania. FAU - Popa, Adrian-Claudiu AU - Popa AC AD - Department of Cardiology, Clinical Emergency Hospital Sfantul Pantelimon, 021659 Bucharest, Romania. FAU - Marcu, Bogdan AU - Marcu B AUID- ORCID: 0009-0001-6451-1446 AD - Department of Cardiology, Clinical Emergency Hospital Sfantul Pantelimon, 021659 Bucharest, Romania. FAU - Enache, Vasilica AU - Enache V AD - Department of Cardiology, Clinical Emergency Hospital Sfantul Pantelimon, 021659 Bucharest, Romania. AD - Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, 020021 Bucharest, Romania. LA - eng PT - Journal Article PT - Review DEP - 20260930 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - 0 (Biomarkers) SB - IM MH - Humans MH - *Atherosclerosis/physiopathology/diagnosis/etiology/genetics MH - Arthritis, Rheumatoid/complications MH - Disease Progression MH - Biomarkers/blood/analysis MH - Lupus Erythematosus, Systemic/complications MH - Antiphospholipid Syndrome/complications MH - Renal Insufficiency, Chronic/complications MH - Risk Factors PMC - PMC13706989 OTO - NOTNLM OT - accelerated atherosclerosis OT - genetic factors OT - rheumatoid arthritis OT - systemic lupus erythematosus OT - therapeutic interventions OT - ultrasonography COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/09/30 CRDT- 2026/10/27 10:42 PHST- 2026/07/30 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - medicina62101898 [pii] AID - medicina-62-01898 [pii] AID - 10.3390/medicina62101898 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Sep 30;62(10):1898. doi: 10.3390/medicina62101898. PMID- 42942428 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Sep 30 TI - AI-Powered Preoperative Chest Radiograph-Derived Cardiovascular Border Indices and Risk of Postoperative Major Adverse Cardiovascular Events: A Large-Scale Retrospective Cohort Study. LID - 10.3390/medicina62101897 [doi] LID - 1897 AB - Background and Objectives: Routine preoperative chest radiography (CXR) has limited value for perioperative risk prediction when interpreted qualitatively. The Automated Diagnosis of Cardiovascular abnormalities (ADC) model enables automated quantification of cardiomediastinal vascular border (CVB) parameters on CXR. This study evaluated the associations of AI-derived CVB metrics with postoperative major adverse cardiovascular events (MACE) and their incremental predictive value beyond clinical factors. Materials and Methods: This retrospective cohort study included patients who underwent surgery under general anesthesia at a tertiary academic center. The ADC model quantified CVB parameters as raw measurements and age- and sex-adjusted z-scores. The primary outcome was postoperative in-hospital MACE. Associations were assessed using multivariable logistic regression, and discrimination was evaluated using receiver operating characteristic curve analysis. Incremental predictive performance was assessed by adding selected CVB parameters to a clinical reference model. Results: Among 101,531 patients, MACE occurred in 1655 patients (1.6%). All ADC-derived CVB parameters remained independently associated with MACE after multivariable adjustment. For cardiothoracic ratio, the adjusted OR was 1.64 (95% CI, 1.58-1.70; p < 0.001), with an AUC of 0.789. For the composite CVB z-score, the adjusted OR was 1.88 (95% CI, 1.79-1.97; p < 0.001), with an AUC of 0.762. Adding either the CT ratio or composite CVB z-score to the clinical reference model increased the AUC from 0.894 to 0.906 (p < 0.001 for both), with similar improvement in temporal validation. Conclusions: AI-derived CVB parameters from preoperative CXR were independently associated with postoperative in-hospital MACE and provided modest incremental predictive information beyond routinely available clinical factors. Automated CVB analysis may provide additional cardiovascular risk information from a routinely obtained preoperative CXR without additional imaging or patient burden, although further external validation is required to establish its clinical utility. FAU - Jang, Hwa-Young AU - Jang HY AD - Department of Anesthesiology and Pain Medicine, Asan Medical Center, Brain Korea 21 Project, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea. FAU - Kim, Jeong Hwan AU - Kim JH AUID- ORCID: 0000-0001-8925-2850 AD - Department of Anesthesiology and Pain Medicine, Asan Medical Center, Brain Korea 21 Project, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea. FAU - Oh, Hong Min AU - Oh HM AD - Department of Radiology (Cardiovascular Imaging), Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea. FAU - Yoon, Hong-Cheol AU - Yoon HC AUID- ORCID: 0009-0001-3074-0312 AD - Department of Anesthesiology and Pain Medicine, Asan Medical Center, Brain Korea 21 Project, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea. FAU - Kim, Chang-Woo AU - Kim CW AUID- ORCID: 0000-0002-3254-4542 AD - Department of Anesthesiology and Pain Medicine, Asan Medical Center, Brain Korea 21 Project, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea. FAU - Kim, Woo-Jin AU - Kim WJ AUID- ORCID: 0000-0002-7273-675X AD - Department of Anesthesiology and Pain Medicine, Asan Medical Center, Brain Korea 21 Project, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea. FAU - Kim, Hyun-Seok AU - Kim HS AD - Big Data Research Center, Asan Institute for Life Science, Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea. FAU - Seo, Woo-Young AU - Seo WY AUID- ORCID: 0000-0002-5444-3320 AD - Biomedical Engineering Research Center, Asan Institute for Life Science, Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea. FAU - Yang, Dong Hyun AU - Yang DH AD - Department of Radiology (Cardiovascular Imaging), Asan Medical Center, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea. AD - Big Data Research Center, Asan Institute for Life Science, Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea. FAU - Kim, Sung-Hoon AU - Kim SH AUID- ORCID: 0000-0001-5215-7585 AD - Department of Anesthesiology and Pain Medicine, Asan Medical Center, Brain Korea 21 Project, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea. AD - Institute of Digital Healthcare, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Republic of Korea. LA - eng GR - 2026IP0054/Asan Institute for Life Sciences/ GR - HI22C1723/Ministry of Health and Welfare/ GR - 2026IP0004/Asan Institute for Life Sciences/ GR - HR20C0026/Ministry of Health and Welfare/ GR - U01 IP000054/IP/NCIRD CDC HHS/United States PT - Journal Article DEP - 20260930 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - Retrospective Studies MH - Female MH - Male MH - Middle Aged MH - *Radiography, Thoracic/methods MH - Aged MH - *Postoperative Complications/epidemiology/etiology MH - *Cardiovascular Diseases MH - Cohort Studies MH - ROC Curve MH - *Artificial Intelligence MH - Logistic Models MH - Risk Assessment/methods MH - Risk Factors MH - Preoperative Care/methods PMC - PMC13707251 OTO - NOTNLM OT - artificial intelligence OT - perioperative care OT - postoperative complications OT - radiography OT - risk assessment OT - thoracic COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/09/30 CRDT- 2026/10/27 10:42 PHST- 2026/08/06 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - medicina62101897 [pii] AID - medicina-62-01897 [pii] AID - 10.3390/medicina62101897 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Sep 30;62(10):1897. doi: 10.3390/medicina62101897. PMID- 42942426 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Sep 30 TI - Bridging the Translational Gap in Artificial Intelligence for Otology and Neurotology: Clinical Readiness, Limitations, and Research Priorities. LID - 10.3390/medicina62101895 [doi] LID - 1895 AB - Background and Objectives: Artificial intelligence (AI) has generated numerous proof-of-concept applications in otology and neurotology, but relatively few have progressed to routine clinical use. This narrative review evaluates not only what AI can do across audiovestibular medicine, but also how close individual applications are to clinical translation and which barriers continue to limit implementation. Materials and Methods: PubMed/MEDLINE, Scopus, and Web of Science were searched from database inception to 31 July 2026 using combinations of terms related to AI, machine learning, deep learning, hearing loss, cochlear implantation, tinnitus, vestibular disorders, nystagmus, temporal bone imaging, digital phenotyping, rehabilitation, and robotic surgery. Reference lists of relevant publications were also screened. Evidence was synthesized thematically and interpreted according to task maturity, external validation, clinical utility, workflow integration, and patient-safety requirements. Results: The most mature applications are narrow, well-defined tasks involving image or signal classification and anatomical segmentation, including audiogram pattern recognition, automated nystagmus extraction, temporal-bone segmentation, and vestibular schwannoma volumetry. By contrast, multimodal prognostic models, AI-guided treatment selection, digital phenotyping, autonomous decision support, and robotic or intraoperative systems remain less clinically established. Across domains, translation is constrained by small retrospective datasets, center- and device-specific acquisition patterns, inconsistent reference standards, limited external and prospective validation, inadequate reporting of calibration and failure modes, and scarce evidence of improved patient outcomes or workflow efficiency. Conclusions: The principal challenge for AI in otology and neurotology is no longer technical feasibility, but demonstration of generalizable clinical value. Future research should prioritize representative multicenter data, independent validation, prospective impact studies, clinically meaningful comparators, interoperability, explainability, fairness, and post-deployment surveillance. AI should be implemented as an auditable, human-supervised technology that strengthens specialist judgment and patient-centered care. FAU - Viola, Pasquale AU - Viola P AUID- ORCID: 0000-0002-2352-1146 AD - Unit of Audiology, Phoniatrics and Vestibology, Regional Centre for Cochlear Implants and ENT Diseases, Department of Experimental and Clinical Medicine, Magna Graecia University, 88100 Catanzaro, Italy. FAU - Aragona, Teodoro AU - Aragona T AD - Department of Otorhinolaryngology and Maxillofacial Surgery, IRCCS Research Hospital Casa Sollievo della Sofferenza, San Giovanni Rotondo, 71013 Foggia, Italy. FAU - De Luca, Pietro AU - De Luca P AUID- ORCID: 0000-0002-6505-1680 AD - Otolaryngology Department, San Giovanni-Addolorata Hospital, 00184 Rome, Italy. FAU - Gioacchini, Federico Maria AU - Gioacchini FM AD - Department of Clinical and Molecular Sciences, Polytechnic University of Marche, 60126 Ancona, Italy. FAU - Scarpa, Alfonso AU - Scarpa A AUID- ORCID: 0000-0001-9219-6175 AD - Department of Medicine and Surgery, University of Salerno, 84081 Baronissi, Italy. FAU - Carvelli, Simona AU - Carvelli S AD - Unit of Audiology, Phoniatrics and Vestibology, Regional Centre for Cochlear Implants and ENT Diseases, Department of Experimental and Clinical Medicine, Magna Graecia University, 88100 Catanzaro, Italy. FAU - Petrolo, Claudio AU - Petrolo C AD - Unit of Audiology, Phoniatrics and Vestibology, Regional Centre for Cochlear Implants and ENT Diseases, Department of Experimental and Clinical Medicine, Magna Graecia University, 88100 Catanzaro, Italy. FAU - Mussari, Roberta AU - Mussari R AD - Unit of Audiology, Phoniatrics and Vestibology, Regional Centre for Cochlear Implants and ENT Diseases, Department of Experimental and Clinical Medicine, Magna Graecia University, 88100 Catanzaro, Italy. FAU - Chiarella, Giuseppe AU - Chiarella G AUID- ORCID: 0000-0002-9829-2229 AD - Unit of Audiology, Phoniatrics and Vestibology, Regional Centre for Cochlear Implants and ENT Diseases, Department of Experimental and Clinical Medicine, Magna Graecia University, 88100 Catanzaro, Italy. LA - eng PT - Journal Article PT - Review DEP - 20260930 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - *Artificial Intelligence/trends MH - *Neurotology/methods/trends MH - *Translational Research, Biomedical MH - *Otolaryngology/methods/trends PMC - PMC13706823 OTO - NOTNLM OT - artificial intelligence OT - clinical translation OT - cochlear implantation OT - digital health OT - external validation OT - hearing loss OT - medical imaging OT - neurotology OT - otology OT - vestibular disorders COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/09/30 CRDT- 2026/10/27 10:42 PHST- 2026/08/06 00:00 [received] PHST- 2026/09/10 00:00 [revised] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - medicina62101895 [pii] AID - medicina-62-01895 [pii] AID - 10.3390/medicina62101895 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Sep 30;62(10):1895. doi: 10.3390/medicina62101895. PMID- 42942425 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Sep 30 TI - Lactobacillus rhamnosus Lysate for Metabolic Dysfunction-Associated Steatotic Liver Disease: A Randomized Controlled Trial. LID - 10.3390/medicina62101893 [doi] LID - 1893 AB - Background and Objectives: Modulation of the gut-liver axis with non-viable microbial components is a promising approach to correct metabolic disorders. The aim of the study was to evaluate the short-term efficacy and safety of the postbiotic lysate Lactobacillus rhamnosus DV-NRRL B-68023 on non-invasive measures of hepatic steatosis, transaminase activity, and anthropometric parameters in patients with MASLD. Materials and Methods: In a randomized, double-blind, placebo-controlled trial (NCT06352697), 52 adults were assigned 1:1 to receive the postbiotic Del-Immune V((R)) Extra (100 mg twice daily; n = 26) or placebo (n = 26) for 3 months followed by a 3-month follow-up without treatment. The registered co-primary endpoints were FLI, HSI, and TyG. Additionally, transaminases, lipid parameters, hs-CRP, and body composition indices were assessed. Results: In the ITT analysis (n = 52), FLI showed a nominal time-by-treatment interaction (p = 0.049), with a between-group difference in change at month 3 of -7.20 points (95% CI: -12.95 to -1.46). HSI and TyG did not differ significantly between groups. In exploratory ITT analyses, waist circumference showed a significant time-by-treatment interaction (p = 0.003), whereas the interaction for visceral fat was not significant (p = 0.079). ALT and AST decreased significantly within the postbiotic group, but the corresponding time-by-treatment interactions were not significant (p = 0.340 and p = 0.111, respectively). Lipid parameters and hs-CRP did not differ significantly between groups. All adverse events were mild and self-limiting. Conclusions: Three months of Lactobacillus rhamnosus lysate DV-NRRL B-68023 was well tolerated. Among the three registered co-primary surrogate indices, only FLI showed a nominal between-group difference; HSI and TyG did not. An exploratory reduction in waist circumference was observed, whereas the ALT and AST changes were not significantly different from placebo. These findings warrant confirmation in larger studies using direct liver assessments and longer follow-up. FAU - Savytska, Maryana AU - Savytska M AD - Physiology Department, Danylo Halytsky Lviv National Medical University, 79010 Lviv, Ukraine. FAU - Ilkiv, Yeva AU - Ilkiv Y AD - Endocrinology Department, Bogomolets National Medical University, 01601 Kyiv, Ukraine. FAU - Baka, Olena AU - Baka O AD - Center for Innovative Medical Technologies, National Academy of Sciences of Ukraine, 04053 Kyiv, Ukraine. FAU - Christian, Elina AU - Christian E AUID- ORCID: 0000-0003-0920-4627 AD - Endocrinology Department, Bogomolets National Medical University, 01601 Kyiv, Ukraine. FAU - Yerokhovych, Viktoriia AU - Yerokhovych V AD - Endocrinology Department, Bogomolets National Medical University, 01601 Kyiv, Ukraine. FAU - Kyriienko, Dmytro AU - Kyriienko D AD - Kyiv City Clinical Endocrinology Center, 01601 Kyiv, Ukraine. FAU - Falalyeyeva, Tetyana AU - Falalyeyeva T AD - Educational and Scientific Centre "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, 01601 Kyiv, Ukraine. AD - Medical Laboratory CSD, Scientific, 02000 Kyiv, Ukraine. FAU - Petakh, Pavlo AU - Petakh P AUID- ORCID: 0000-0002-0860-4445 AD - Department of Biochemistry and Pharmacology, Uzhhorod National University, 88000 Uzhhorod, Ukraine. AD - KSE Health, Aging and Diseases Center, Kyiv School of Economics, 02000 Kyiv, Ukraine. FAU - Halabitska, Iryna AU - Halabitska I AUID- ORCID: 0000-0002-9028-7230 AD - Department of Therapy and Family Medicine, I. Horbachevsky Ternopil National Medical University, 46001 Ternopil, Ukraine. FAU - Kamyshnyi, Oleksandr AU - Kamyshnyi O AUID- ORCID: 0000-0003-3141-4436 AD - KSE Health, Aging and Diseases Center, Kyiv School of Economics, 02000 Kyiv, Ukraine. AD - Department of Therapy and Family Medicine, I. Horbachevsky Ternopil National Medical University, 46001 Ternopil, Ukraine. FAU - Sichel, Liubov AU - Sichel L AUID- ORCID: 0009-0005-9964-0378 AD - MirImmunoPharm, 04111 Kyiv, Ukraine. AD - Stellar Biotics, Rockleigh, NJ 07647, USA. FAU - Kobyliak, Nazarii AU - Kobyliak N AUID- ORCID: 0000-0001-9814-689X AD - Endocrinology Department, Bogomolets National Medical University, 01601 Kyiv, Ukraine. AD - Medical Laboratory CSD, Scientific, 02000 Kyiv, Ukraine. LA - eng PT - Journal Article PT - Randomized Controlled Trial DEP - 20260930 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - *Lacticaseibacillus rhamnosus MH - Female MH - Double-Blind Method MH - Middle Aged MH - Male MH - *Probiotics/therapeutic use MH - Adult MH - *Fatty Liver/drug therapy MH - *Metabolic Diseases/drug therapy MH - Treatment Outcome PMC - PMC13706883 OTO - NOTNLM OT - MASLD OT - fatty liver index OT - gut microbiota OT - hepatic steatosis index OT - metabiotics OT - postbiotics OT - probiotic derivatives OT - triglyceride-to-glucose index COIS- Author L.S. is employed by MirImmunoPharm LLC and is a co-founder, Chief Executive Officer (CEO), and Chief Scientific Officer (CSO) of Stellar Biotics LLC. The active study product and matching placebo were supplied in kind by MirImmunoPharm LLC. No external financial funding was received. Author L.S. contributed to conceptualization, supervision, project administration, and writing-review and editing, as detailed in the Author Contributions statement. These contributions were made in her capacity as an investigator. Apart from supplying the study products, MirImmunoPharm LLC had no role in participant recruitment, data collection, access to the study database, statistical analysis, interpretation of the results, manuscript preparation, or the decision to submit the manuscript. Stellar Biotics LLC provided no financial or in-kind support and had no role in participant recruitment, data collection, access to the study database, statistical analysis, interpretation of the results, manuscript preparation, or the decision to submit the manuscript. Author N.K. had full access to the complete study dataset. Formal statistical analysis was performed by Y.I. The remaining authors declare no other commercial or financial relationships relevant to this study. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/09/30 CRDT- 2026/10/27 10:42 PHST- 2026/08/19 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - medicina62101893 [pii] AID - medicina-62-01893 [pii] AID - 10.3390/medicina62101893 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Sep 30;62(10):1893. doi: 10.3390/medicina62101893. PMID- 42942424 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Sep 30 TI - Bone Health in Triathletes: Skeletal Loading, Low Energy Availability, and Bone Stress Injury-A Critical Narrative Review. LID - 10.3390/medicina62101894 [doi] LID - 1894 AB - Background and Objectives: Triathlon combines low-impact swimming and cycling with repetitive running and high energy demand. We evaluated triathlon-specific evidence on skeletal adaptation, low energy availability (LEA), Relative Energy Deficiency in Sport (REDs), and bone stress injury (BSI). Materials and Methods: We purposively selected evidence from PubMed/MEDLINE through 11 August 2026 and citation tracking. The search returned 233 records; 25 primary reports representing approximately 20-22 independent cohorts were synthesized, with the consensus literature providing the context. Results: Most studies were small, cross-sectional, and heterogeneous. No uniform bone mineral density deficit emerged; findings varied by site, sex, age, body size, competitive level, race distance, and modality. One four-year cohort of 50 elite short-course athletes found substantial BSI burden and a higher rate in females, but its single-program setting limits generalization. Evidence on female LEA/Triad/REDs relied mainly on questionnaires, dietary assessments, cross-sectional imaging, and case reports; evidence on males and interventions was sparse. Biomarkers, areal bone mineral density, structural imaging, risk screens, and BSI were not interchangeable. Conclusions: Evidence does not support universal imaging, biomarker testing, or supplementation; individualized assessment is preferable. Prospective, preregistered, sex- and age-inclusive studies should align discipline-specific exposure, repeated energy assessment, site-specific skeletal measures, and imaging-verified BSI. FAU - Lin, Po-Yuan AU - Lin PY AD - Department of Orthopedics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970473, Taiwan. FAU - Su, Ooi Yean AU - Su OY AD - Department of Medical Education, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970473, Taiwan. FAU - Wu, Wen-Tien AU - Wu WT AUID- ORCID: 0000-0001-6945-712X AD - Department of Orthopedics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970473, Taiwan. AD - Institute of Medical Sciences, Tzu Chi University, Hualien 970374, Taiwan. AD - School of Medicine, Tzu Chi University, Hualien 970374, Taiwan. FAU - Lee, Ru-Ping AU - Lee RP AUID- ORCID: 0000-0001-6624-5893 AD - Institute of Medical Sciences, Tzu Chi University, Hualien 970374, Taiwan. FAU - Yao, Ting-Kuo AU - Yao TK AD - Department of Orthopedics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970473, Taiwan. FAU - Yeh, Kuang-Ting AU - Yeh KT AUID- ORCID: 0000-0002-6154-2167 AD - Department of Orthopedics, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970473, Taiwan. AD - Department of Medical Education, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970473, Taiwan. AD - Institute of Medical Sciences, Tzu Chi University, Hualien 970374, Taiwan. AD - School of Medicine, Tzu Chi University, Hualien 970374, Taiwan. AD - Graduate Institute of Clinical Pharmacy, Tzu Chi University, Hualien 970374, Taiwan. LA - eng PT - Journal Article PT - Review DEP - 20260930 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - *Bone Density/physiology MH - *Swimming/physiology MH - *Athletes/statistics & numerical data MH - *Running/physiology/injuries MH - *Bicycling/physiology/injuries MH - *Bone and Bones/injuries MH - Female MH - *Fractures, Stress MH - Male MH - Relative Energy Deficiency in Sport PMC - PMC13706682 OTO - NOTNLM OT - Relative Energy Deficiency in Sport OT - bone health OT - bone mineral density OT - bone stress injury OT - endurance athlete OT - low energy availability OT - narrative review OT - triathlon COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/09/30 CRDT- 2026/10/27 10:42 PHST- 2026/09/14 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - medicina62101894 [pii] AID - medicina-62-01894 [pii] AID - 10.3390/medicina62101894 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Sep 30;62(10):1894. doi: 10.3390/medicina62101894. PMID- 42942414 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Sep 29 TI - Three-Year Functional Vision, Longitudinal Refractive Outcomes, and Patient-Reported Outcomes After Bilateral Implantation of the Extend Bessel-Optics Intraocular Lens: An Independent Real-World Ambispective Study. LID - 10.3390/medicina62101883 [doi] LID - 1883 AB - Background and Objectives: Independent evidence on the longer-term performance of the Extend Bessel-optics intraocular lens remains limited. This study aimed to evaluate refractive and visual changes beyond the first postoperative year and to characterize binocular functional vision, defocus performance, and patient-reported outcomes 36-48 months after bilateral implantation. Materials and Methods: This ambispective, single-center case series included patients who had undergone bilateral implantation of the non-toric Extend IOL with emmetropic targets and had complete one-year postoperative data. One-year refraction and visual acuity data were retrieved retrospectively; eligible patients who had reached at least 36 months after surgery underwent prospective evaluation including refraction, visual acuity, defocus curves, pupillometry, VF-14, spectacle use, dysphotopsia, and satisfaction. Results: Of 37 patients in the source cohort, two lacked the required one-year postoperative visit. All 35 eligible patients (70 eyes) completed the prospective evaluation at 36-48 months. No statistically significant change in subjective spherical equivalent was detected (mean paired change, +0.029 D; 95% CI, -0.005 to +0.062 D; p = 0.114). Small statistically significant changes occurred in objective spherical equivalent (-0.052 D) and UDVA, UIVA, and UNVA (+0.009, +0.027, and +0.025 logMAR, respectively). Mean binocular defocus acuity remained /=10 buds according to ITBCC criteria). Mucinous histology was evaluated as a separate subgroup analysis. The primary endpoint was recurrence-free survival (RFS); secondary endpoints were disease-free survival (DFS) and overall survival (OS). Competing risks analysis was performed using the Aalen-Johansen estimator, with death without recurrence as the competing event. Results: The median follow-up was 43.7 months. Five-year RFS rates were 97.1%, 92.8%, and 70.0% in the low-, intermediate-, and high-risk groups, respectively (log-rank p = 0.041). Five-year OS rates were 87.2%, 88.6%, and 69.9%, respectively (log-rank p = 0.0004). Five-year cumulative incidence of recurrence in competing risks analysis was 2.9%, 7.1%, and 26.0%, respectively. Five-year DFS rates were 87.2%, 83.1%, and 54.2% (log-rank p = 0.0003). In multivariate DFS analysis, intestinal obstruction (HR = 4.26; 95% CI: 2.05-8.87; p < 0.001) and tumor budding (HR = 3.77; 95% CI: 1.67-8.53; p = 0.001) were independent prognostic factors; consistent but exploratory RFS estimates were observed (obstruction HR = 8.51; 95% CI: 2.50-28.96; p = 0.001; tumor budding HR = 5.48; 95% CI: 1.72-17.44; p = 0.004), derived from 12 recurrence events and therefore prone to overfitting. As a continuous variable, each 1-unit increase in the seven-factor risk score increased the RFS hazard 1.74-fold (95% CI: 1.21-2.51; p = 0.003; RFS C-index = 0.656; 95% CI: 0.489-0.830). A parsimonious two-factor model (obstruction + tumor budding) achieved a higher C-index of 0.760 (95% CI: 0.569-0.921) for RFS. Mucinous histology was present in 17 patients (9.8%) and showed no recurrence events. Adjuvant CT rates increased significantly with higher risk scores (p = 0.002). Conclusions: Guideline-based seven-factor risk stratification demonstrates statistically significant, though modest, discriminatory performance (RFS C-index = 0.656; 95% CI: 0.489-0.830; DFS C-index = 0.646; 95% CI: 0.519-0.778) in pMMR T3N0 colon cancer. Intestinal obstruction and tumor budding are independent prognostic factors that effectively stratify patients by recurrence risk; a parsimonious two-factor model based on these two features matched or exceeded the discrimination of the full score. The seven-factor system should be regarded as a validation of guideline-defined high-risk features that may support adjuvant chemotherapy discussions in stage IIA colon cancer; external validation is required before clinical implementation. FAU - Celebi, Abdussamet AU - Celebi A AUID- ORCID: 0000-0002-6922-1018 AD - Department of Medical Oncology, Faculty of Medicine, Marmara University, Istanbul 34899, Turkiye. FAU - Meral, Eren AU - Meral E AD - Department of Internal Medicine, Faculty of Medicine, Marmara University, Istanbul 34899, Turkiye. FAU - Akagunduz, Firat AU - Akagunduz F AUID- ORCID: 0000-0001-7969-9464 AD - Department of Medical Oncology, Faculty of Medicine, Marmara University, Istanbul 34899, Turkiye. FAU - Demirel, Ahmet AU - Demirel A AUID- ORCID: 0000-0001-8162-8294 AD - Department of Medical Oncology, Faculty of Medicine, Marmara University, Istanbul 34899, Turkiye. FAU - Tunc, Mustafa Alperen AU - Tunc MA AUID- ORCID: 0000-0002-1537-3032 AD - Department of Medical Oncology, Faculty of Medicine, Marmara University, Istanbul 34899, Turkiye. FAU - Pacaci, Burak AU - Pacaci B AD - Department of Medical Oncology, Faculty of Medicine, Marmara University, Istanbul 34899, Turkiye. FAU - Kocaaslan, Erkam AU - Kocaaslan E AUID- ORCID: 0000-0002-8994-2904 AD - Department of Medical Oncology, Faculty of Medicine, Marmara University, Istanbul 34899, Turkiye. FAU - Guren, Ali Kaan AU - Guren AK AUID- ORCID: 0000-0002-3562-5006 AD - Department of Medical Oncology, Faculty of Medicine, Marmara University, Istanbul 34899, Turkiye. FAU - Isik, Selver AU - Isik S AUID- ORCID: 0000-0002-2726-1740 AD - Department of Medical Oncology, Faculty of Medicine, Marmara University, Istanbul 34899, Turkiye. FAU - Coban, Ezgi AU - Coban E AUID- ORCID: 0000-0003-3309-7972 AD - Department of Medical Oncology, Faculty of Medicine, Marmara University, Istanbul 34899, Turkiye. FAU - Demircan, Nazim Can AU - Demircan NC AD - Department of Medical Oncology, Faculty of Medicine, Marmara University, Istanbul 34899, Turkiye. FAU - Kostek, Osman AU - Kostek O AD - Department of Medical Oncology, Faculty of Medicine, Marmara University, Istanbul 34899, Turkiye. FAU - Bayoglu, Ibrahim Vedat AU - Bayoglu IV AUID- ORCID: 0000-0002-0481-1084 AD - Department of Medical Oncology, Faculty of Medicine, Marmara University, Istanbul 34899, Turkiye. FAU - Sari, Murat AU - Sari M AUID- ORCID: 0000-0003-0596-1559 AD - Department of Medical Oncology, Faculty of Medicine, Marmara University, Istanbul 34899, Turkiye. LA - eng PT - Journal Article DEP - 20260924 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - Retrospective Studies MH - Male MH - Prognosis MH - Female MH - *Colonic Neoplasms/pathology/mortality/diagnosis MH - Risk Assessment/methods/standards MH - Aged MH - Middle Aged MH - Neoplasm Staging/methods MH - Risk Factors MH - Disease-Free Survival MH - Aged, 80 and over MH - Adenocarcinoma PMC - PMC13706934 OTO - NOTNLM OT - T3N0 colon cancer OT - adjuvant chemotherapy OT - competing risks OT - disease-free survival OT - intestinal obstruction OT - recurrence-free survival OT - risk scoring OT - stage IIA OT - tumor budding COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/09/24 CRDT- 2026/10/27 10:42 PHST- 2026/08/01 00:00 [received] PHST- 2026/09/13 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - medicina62101846 [pii] AID - medicina-62-01846 [pii] AID - 10.3390/medicina62101846 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Sep 24;62(10):1846. doi: 10.3390/medicina62101846. PMID- 42942376 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Sep 24 TI - Ultra-Short ECG Indices in Patients Presenting with Epigastric Pain-An Orientation Tool for Diagnosis in the Emergency Department. LID - 10.3390/medicina62101845 [doi] LID - 1845 AB - Background and Objectives: Abdominal pain, particularly epigastric pain, is a leading cause of emergency department (ED) visits and encompasses a wide differential diagnosis, ranging from mild conditions such as gastritis and reflux disease to life-threatening etiologies such as acute abdomen and acute myocardial infarction (MI). Epigastric pain is often associated with stress, inflammation, and visceral signaling, processes mediated by the autonomic nervous system (ANS). Changes in ANS activity can be captured by heart rate variability (HRV), a non-invasive measure derived from cardiac rhythm. In this study, we conducted retrospective research to evaluate the prognostic significance of HRV in ED patients presenting with epigastric pain. Materials and Methods: Retrospective analysis of patients diagnosed with epigastric pain in the ED at Rambam Health Care Campus in 2014. Ultra-short heart rate variability (usHRV) indices were calculated from a 10 s ED electrocardiogram and correlated with mortality, disease severity, and disease origin. Results: 300 patients were included in this study. Due to a very low short-term mortality rate, usHRV could not be reliably assessed for this outcome. The mortality rate throughout the follow-up period of the study was 17.4%, with a median of 8.4 years. No significant association was found between long-term mortality and standard deviation of normal-to-normal intervals (SDNN) or root mean square of successive differences (RMSSD). In multivariate logistic analysis, high RMSSD significantly increased the risk for acute abdomen (RMSSD-adjusted odds ratio (AOR): 1.021, p-value: 0.022, Area under the curve (AUC): 73%), while high RMSSD decreased the risk for acute MI (RMSSD AOR: 0.807, p-value: 0.026, AUC: 83.6%). Conclusions: Ultra-short HRV did not predict long-term mortality in patients presenting with epigastric pain. However, RMSSD was independently associated with both acute abdomen and acute MI. These findings suggest that usHRV parameters may serve as a supportive tool in the ED to aid differentiation between abdominal and cardiac causes of epigastric pain, particularly in high-risk clinical scenarios. FAU - Izhaki, Niv AU - Izhaki N AD - Internal Medicine A Department, Rambam Health Care Campus, Haifa 3109601, Israel. AD - Emergency Medicine Department, Rambam Health Care Campus, Haifa 3109601, Israel. FAU - Perek, Shay AU - Perek S AUID- ORCID: 0000-0002-7280-4062 AD - Internal Medicine A Department, Rambam Health Care Campus, Haifa 3109601, Israel. AD - Emergency Medicine Department, Rambam Health Care Campus, Haifa 3109601, Israel. AD - The Ruth and Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa 3200003, Israel. FAU - Safadi, Shiraz AU - Safadi S AD - The Ruth and Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa 3200003, Israel. FAU - Raz-Pasteur, Ayelet AU - Raz-Pasteur A AD - Internal Medicine A Department, Rambam Health Care Campus, Haifa 3109601, Israel. AD - The Ruth and Bruce Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa 3200003, Israel. LA - eng PT - Journal Article DEP - 20260924 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - Retrospective Studies MH - *Electrocardiography/methods MH - Emergency Service, Hospital/organization & administration/statistics & numerical data MH - *Abdominal Pain/diagnosis/physiopathology/etiology MH - Female MH - Middle Aged MH - Male MH - *Heart Rate/physiology MH - Adult MH - Aged MH - ROC Curve MH - Prognosis MH - Diagnosis, Differential PMC - PMC13707026 OTO - NOTNLM OT - autonomic nervous system (ANS) OT - emergency department (ED) OT - epigastric pain OT - myocardial infarction (MI) OT - ultra-short heart rate variability (usHRV) COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/09/24 CRDT- 2026/10/27 10:42 PHST- 2026/07/28 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - medicina62101845 [pii] AID - medicina-62-01845 [pii] AID - 10.3390/medicina62101845 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Sep 24;62(10):1845. doi: 10.3390/medicina62101845. PMID- 42942374 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Sep 23 TI - Occlusive Myocardial Infarction: A Practical Review of STEMI-Negative Coronary Occlusion in Acute Coronary Syndromes. LID - 10.3390/medicina62101843 [doi] LID - 1843 AB - ST-segment elevation myocardial infarction (STEMI) and non-ST-segment elevation myocardial infarction (NSTEMI) remain the dominant operational categories for acute coronary syndrome triage because they are simple, widely implemented, and closely linked to reperfusion pathways. However, diagnostic ST-segment elevation and acute coronary occlusion are not synonymous. A clinically important subgroup of patients classified as NSTEMI have total or near-total culprit-artery occlusion and may experience delayed angiography despite ongoing myocardial ischemia. This structured narrative review examines the rationale, evidence, electrocardiographic patterns, quantitative bedside criteria, and emerging technologies relevant to occlusion myocardial infarction (OMI), with particular emphasis on STEMI-negative coronary occlusion. Conventional STEMI criteria are highly specific but incompletely sensitive for acute coronary occlusion, particularly in posterior or circumflex infarction, early anterior occlusion, left bundle branch block, ventricular paced rhythm, and transient reperfusion. Recognition of OMI therefore requires integration of ECG morphology, reciprocal changes, serial ECG evolution, clinical presentation, troponin kinetics, and adjunctive imaging rather than reliance on ST-segment thresholds alone. Practical patterns include hyperacute T waves, the de Winter pattern, posterior OMI, modified Sgarbossa-positive conduction abnormalities, subtle anterior OMI, and selected high-risk ischemic patterns. Artificial intelligence may improve scalable detection of subtle occlusion patterns, although external validation, workflow integration, explainability, and prospective outcome studies remain necessary. Current evidence supports OMI as a promising complementary diagnostic framework rather than an established replacement for guideline-based STEMI/NSTEMI classification. Prospective implementation studies are required to determine whether OMI-guided pathways improve clinical outcomes without unacceptable false-positive catheterization-laboratory activation. FAU - Mashiah, Gil AU - Mashiah G AD - Department of Cardiology, Tel Aviv Sourasky Medical Center, Tel Aviv 6423906, Israel. FAU - Elbassel, Reem AU - Elbassel R AD - Department of Cardiology, Tel Aviv Sourasky Medical Center, Tel Aviv 6423906, Israel. FAU - Zornitzki, Lior AU - Zornitzki L AUID- ORCID: 0000-0003-3245-2653 AD - Division of Cardiology, Schulich Heart Centre, Sunnybrook Health Sciences Centre, Toronto, ON M4N 3M5, Canada. AD - Gray Faculty of Medical and Health Sciences, Tel Aviv University, Tel Aviv 6997801, Israel. LA - eng PT - Journal Article PT - Review DEP - 20260923 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - Electrocardiography/methods MH - *Acute Coronary Syndrome/physiopathology/diagnosis/complications MH - *Coronary Occlusion/diagnosis/physiopathology MH - *ST Elevation Myocardial Infarction/diagnosis/physiopathology MH - *Myocardial Infarction/diagnosis PMC - PMC13707315 OTO - NOTNLM OT - STEMI equivalent OT - acute coronary syndrome OT - acute myocardial infarction OT - artificial intelligence OT - coronary occlusion OT - electrocardiography OT - occlusion myocardial infarction OT - reperfusion COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/09/23 CRDT- 2026/10/27 10:42 PHST- 2026/07/09 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - medicina62101843 [pii] AID - medicina-62-01843 [pii] AID - 10.3390/medicina62101843 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Sep 23;62(10):1843. doi: 10.3390/medicina62101843. PMID- 42942361 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Sep 23 TI - Traumatic Intracranial Vascular Lesions in Adults Undergoing Vascular Imaging After Traumatic Brain Injury: A Systematic Review and Meta-Analysis. LID - 10.3390/medicina62101830 [doi] LID - 1830 AB - Background and Objectives: Traumatic intracranial vascular lesions are rare but severe complications of traumatic brain injury (TBI) and include arterial and venous injuries such as arterial dissections, pseudoaneurysms, arterial occlusions, venous sinus thrombosis, arteriovenous fistulas, and carotid-cavernous fistulas. Despite their clinical significance, the available literature remains limited and heterogeneous. This systematic review and meta-analysis aimed to evaluate the diagnostic yield of vascular imaging, spectrum, and imaging modalities of traumatic intracranial vascular lesions in adults with TBI. Materials and Methods: A systematic literature search was conducted from database inception to 31 August 2026 in PubMed, Web of Science Core Collection, Scopus, and the Cochrane Library (CENTRAL) according to the PRISMA 2020 guidelines. Cohorts of patients who underwent vascular imaging after TBI were eligible. When the full text was available, the denominator was the number of patients imaged with a modality able to detect the lesion in question and the numerator was reported at patient level for intracranial lesions. The primary analyses were restricted to cohorts with an explicit adult age criterion or a separately reported adult subgroup; cohorts with a pediatric fraction that could not be separated entered a sensitivity analysis only. Data extraction and quality assessment using the Newcastle-Ottawa Scale and the Joanna Briggs Institute checklist for prevalence studies were performed independently by two reviewers. Proportions were pooled with a logit-transformed random-effects model with restricted maximum-likelihood estimation and prediction intervals, with Freeman-Tukey and generalized linear mixed models as sensitivity analyses. Results: Nineteen studies with 3542 imaged patients were included, eight of them adult cohorts with 2021 patients. In adult cohorts, the pooled diagnostic yield was 11.0% (95% CI, 2.3-39.8%; four cohorts) for dural venous sinus thrombosis, 4.8% (95% CI, 1.1-18.4%; three cohorts) for pseudoaneurysm, 1.1% (95% CI, 0.0-20.8%; two cohorts) for arteriovenous fistula, and 0.7% (95% CI, 0.2-3.1%; three cohorts) for carotid-cavernous fistula, with I(2) between 74.7% and 96.2% and prediction intervals spanning most of the possible range; arterial dissection (7.0%) and arterial occlusion (5.8%) were reported by a single adult cohort and were not pooled. The proportion of patients with any lesion was not pooled, because the seven cohorts that imaged every patient differed in whether and how the venous compartment was assessed; it ranged from 1.5% to 56.9% by cohort. Adding the eleven adult-dominant cohorts gave 17.5% for sinus thrombosis and 4.5% for pseudoaneurysm. In exploratory univariable meta-regressions for sinus thrombosis, trauma mechanism, imaging modality, and study design were each associated with part of the heterogeneity and were confounded with one another in these cohorts. Conclusions: Every estimate describes the yield of vascular imaging in adults selected for it, rests on two-to-four small cohorts, and carries very low certainty; the prediction intervals show that a new cohort may fall far from the pooled value, and none of the estimates can be read as the frequency of these lesions in TBI in general. FAU - Lupascu, Iulia T AU - Lupascu IT AD - Department of Legal Medicine and Bioethics, "Carol Davila University of Medicine and Pharmacy" Bucharest, 050474 Bucharest, Romania. AD - Clinical Emergency Hospital Bucharest, 014461 Bucharest, Romania. FAU - Minoiu, Costin A AU - Minoiu CA AUID- ORCID: 0000-0002-9037-2494 AD - Clinical Emergency Hospital Bucharest, 014461 Bucharest, Romania. AD - Department of Radiology, "Carol Davila University of Medicine and Pharmacy" Bucharest, 050474 Bucharest, Romania. FAU - Popa, Bogdan V AU - Popa BV AUID- ORCID: 0009-0004-5993-4761 AD - Clinical Emergency Hospital Bucharest, 014461 Bucharest, Romania. AD - Department of Radiology, "Carol Davila University of Medicine and Pharmacy" Bucharest, 050474 Bucharest, Romania. FAU - Homentcovschi, Corina AU - Homentcovschi C AUID- ORCID: 0009-0003-9396-4975 AD - Department of Medical Semiology "Carol Davila University of Medicine and Pharmacy" Bucharest, 050474 Bucharest, Romania. AD - Clinical Hospital Coltea, 030167 Bucharest, Romania. FAU - Florescu, Lucian M AU - Florescu LM AD - Department of Radiology, "University of Medicine and Pharmacy of Craiova", 200349 Craiova, Romania. AD - Clinical Emergency Hospital Craiova, 200642 Craiova, Romania. FAU - Hostiuc, Sorin AU - Hostiuc S AUID- ORCID: 0000-0003-4130-9402 AD - The National Institute of Forensic Medicine "Mina Minovici", 077160 Bucharest, Romania. AD - Department of Legal Medicine and Bioethics, Faculty of Dental Medicine, "Carol Davila University of Medicine and Pharmacy", 020021 Bucharest, Romania. LA - eng PT - Journal Article PT - Meta-Analysis PT - Review PT - Systematic Review DEP - 20260923 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - *Brain Injuries, Traumatic/complications MH - Adult PMC - PMC13706739 OTO - NOTNLM OT - arterial dissection OT - arteriovenous fistula OT - carotid-cavernous fistula OT - intracranial vascular lesions OT - pseudoaneurysm OT - traumatic brain injury OT - traumatic intracranial vascular lesions OT - venous sinus thrombosis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/09/23 CRDT- 2026/10/27 10:42 PHST- 2026/08/18 00:00 [received] PHST- 2026/09/08 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - medicina62101830 [pii] AID - medicina-62-01830 [pii] AID - 10.3390/medicina62101830 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Sep 23;62(10):1830. doi: 10.3390/medicina62101830. PMID- 42942357 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Sep 22 TI - Autologous Collagen-Induced Chondrogenesis (ACIC) for Osteochondral Lesions of the Talus: A Systematic Review and Meta-Analysis. LID - 10.3390/medicina62101826 [doi] LID - 1826 AB - Background and Objectives: Autologous Collagen-Induced Chondrogenesis (ACIC) is a regenerative technique for treating osteochondral lesions that combines microfracture with the application of an injectable atelocollagen scaffold over the defect. This scaffold stabilizes the marrow clot and promotes the retention of mesenchymal stem cells at the repair site. Its use for Osteochondral Lesions of the Talus (OLTs) has increased in recent years, with several studies reporting favorable clinical outcomes. However, the available evidence has not yet been comprehensively synthesized. The aim of this systematic review and meta-analysis was to evaluate the clinical outcomes of ACIC for the treatment of OLTs. Materials and Methods: This study was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Studies evaluating ACIC for the treatment of OLTs were systematically identified and selected. Data regarding patient characteristics, surgical technique, and clinical outcomes were extracted and analyzed. Results: Five studies were included in the systematic review, comprising 97 patients (50 males and 47 females) with a mean age of 40.9 years (range, 25.6-48.6 years). Meta-analysis demonstrated a significant improvement in functional outcomes, with an estimated Mean Difference (MD) in American Orthopaedic Foot & Ankle Society (AOFAS) scores of 26.54 points (95% CI, 17.34-35.74; p < 0.001). Pain outcomes also significantly improved, with a pooled MD in Visual Analogue Scale (VAS) scores of -3.72 points (95% CI, -4.44 to -3.01; p < 0.001). Conclusions: ACIC for OLTs provides consistent improvements in pain and functional outcomes across the available studies. The finding supported by the available data is that postoperative scores improved in small, predominantly uncontrolled cohorts, but the incremental benefit attributable to ACIC over conventional microfracture alone remains uncertain. Therefore, further adequately powered prospective studies directly comparing ACIC with microfracture alone are needed to better establish its clinical efficacy. FAU - Artioli, Elena AU - Artioli E AUID- ORCID: 0000-0001-9279-4722 AD - Department of Biomedical and Neuromotor Sciences (DIBINEM), Alma Mater Studiorum University of Bologna, 40123 Bologna, Italy. FAU - Mazzotti, Antonio AU - Mazzotti A AUID- ORCID: 0000-0001-9974-4787 AD - Department of Biomedical and Neuromotor Sciences (DIBINEM), Alma Mater Studiorum University of Bologna, 40123 Bologna, Italy. AD - 1st Orthopaedics and Traumatologic Clinic, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy. FAU - Faldini, Cesare AU - Faldini C AUID- ORCID: 0000-0001-8152-4778 AD - Department of Biomedical and Neuromotor Sciences (DIBINEM), Alma Mater Studiorum University of Bologna, 40123 Bologna, Italy. AD - 1st Orthopaedics and Traumatologic Clinic, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy. LA - eng PT - Journal Article PT - Meta-Analysis PT - Systematic Review DEP - 20260922 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - 9007-34-5 (Collagen) SB - IM MH - Humans MH - *Chondrogenesis/physiology/drug effects MH - *Collagen/therapeutic use/pharmacology MH - *Talus/injuries/surgery/physiopathology PMC - PMC13706983 OTO - NOTNLM OT - ACIC OT - ankle OT - cartilage OT - osteochondral lesions of the talus COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/09/22 CRDT- 2026/10/27 10:42 PHST- 2026/08/17 00:00 [received] PHST- 2026/09/09 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - medicina62101826 [pii] AID - medicina-62-01826 [pii] AID - 10.3390/medicina62101826 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Sep 22;62(10):1826. doi: 10.3390/medicina62101826. PMID- 42942356 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Sep 22 TI - Associations of TNF-alpha, Uric Acid, and the Leptin/Adiponectin Ratio with HOMA-IR in Hospitalized Adults: A Cross-Sectional Secondary Analysis of 147 Patients. LID - 10.3390/medicina62101825 [doi] LID - 1825 AB - Background and Objectives: Anthropometric measures incompletely capture the inflammatory and adipokine heterogeneity underlying insulin resistance. We examined associations of tumor necrosis factor-alpha (TNF-alpha), serum uric acid, and the leptin/adiponectin ratio with HOMA-IR after adjustment. Materials and Methods: This single-center cross-sectional secondary analysis included 147 of 239 hospitalized adults with HOMA-IR and predefined core data; fasting glucose, insulin, and biomarker testing was elective and patient-funded. Continuous ln(HOMA-IR) was the primary outcome. A secondary upper-quartile phenotype (HOMA-IR >/= 4.78; n = 37) was cohort-specific and non-diagnostic. Robust linear regression used HC3 standard errors. Sensitivity analyses excluded T2DM and replaced BMI with waist circumference or visceral fat area. Binary and AUC analyses were exploratory (expanded complete-case model, n = 112). Results: Each doubling of (TNF-alpha + 1) was associated with a 10.2% higher HOMA-IR in the expanded model (p = 0.021). The association persisted after excluding T2DM (12.1% higher HOMA-IR per doubling; n = 81; p = 0.012) and when BMI was replaced by waist circumference (p = 0.022) or visceral fat area (p = 0.018). Uric acid and the leptin/adiponectin ratio did not reach statistical significance in the adjusted continuous-outcome model. The 112 complete cases and 35 incomplete cases were broadly similar in measured baseline characteristics, although uric acid was higher among complete cases (p = 0.020). Conclusions: In this selected hospitalized cohort, TNF-alpha showed the most consistent adjusted association with the observed fasting glucose-insulin phenotype. The binary and discrimination analyses remain preliminary and do not establish diagnostic or clinical prediction utility. Prospective external validation is required. FAU - Tischer, Alina Andreea AU - Tischer AA AD - Ear-Nose-Throat Department, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. FAU - Stana, Loredana Gabriela AU - Stana LG AUID- ORCID: 0000-0002-1784-9209 AD - Department I, Discipline of Anatomy and Embryology, Faculty of Medicine, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041 Timisoara, Romania. FAU - Ilie, Ovidiu Calin AU - Ilie OC AD - Department I, Discipline of Dental Prostheses Technology (Dental Technology), Faculty of Dental Medicine, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. FAU - Ilie, Adrian-Cosmin AU - Ilie AC AD - Department of Public Health and Sanitary Management, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041 Timisoara, Romania. FAU - Craciun, Lavinia AU - Craciun L AD - Department I, Discipline of Anatomy and Embryology, Faculty of Medicine, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041 Timisoara, Romania. FAU - Capraru, Ionut AU - Capraru I AD - Department of Epidemiology, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041 Timisoara, Romania. FAU - Clej, Emilia Elena AU - Clej EE AD - Doctoral School, Department of General Medicine, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041 Timisoara, Romania. LA - eng PT - Journal Article DEP - 20260922 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 RN - 0 (Leptin) RN - 0 (Tumor Necrosis Factor-alpha) RN - 268B43MJ25 (Uric Acid) RN - 0 (Adiponectin) RN - 0 (Biomarkers) RN - 0 (Blood Glucose) SB - IM MH - Humans MH - Cross-Sectional Studies MH - Female MH - Male MH - *Leptin/blood/analysis MH - *Tumor Necrosis Factor-alpha/blood/analysis MH - Middle Aged MH - *Uric Acid/blood/analysis MH - *Insulin Resistance/physiology MH - *Adiponectin/blood/analysis MH - Aged MH - Biomarkers/blood/analysis MH - Adult MH - Blood Glucose/analysis MH - Body Mass Index MH - Hospitalization/statistics & numerical data PMC - PMC13706978 OTO - NOTNLM OT - HOMA-IR OT - adiponectin OT - cardiovascular risk OT - insulin resistance OT - leptin OT - tumor necrosis factor-alpha OT - type 2 diabetes OT - uric acid COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/09/22 CRDT- 2026/10/27 10:42 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - medicina62101825 [pii] AID - medicina-62-01825 [pii] AID - 10.3390/medicina62101825 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Sep 22;62(10):1825. doi: 10.3390/medicina62101825. PMID- 42942354 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1648-9144 (Electronic) IS - 1010-660X (Print) IS - 1010-660X (Linking) VI - 62 IP - 10 DP - 2026 Sep 22 TI - Exhaled Breath Temperature in COPD. LID - 10.3390/medicina62101823 [doi] LID - 1823 AB - Chronic obstructive pulmonary disease (COPD) is a leading cause of morbidity and mortality worldwide. Identifying patients with early COPD remains challenging; however, even at this stage, substantial biological disease progression and irreversible lung injury may already be present. Considerable effort has therefore been directed toward identifying parameters that detect early pathophysiological changes before airflow limitation develops. The importance of this stage has recently been recognized and is described using terms such as "pre-COPD" and "early COPD". Exhaled breath temperature (EBT) is a non-invasive method for detecting and monitoring respiratory inflammation. Most EBT studies have been conducted in asthma and have demonstrated the utility of this approach for assessing changes in airway inflammation. In patients with COPD, the number of airways and their vasculature is reduced, and EBT decreases proportionally with the degree of structural destruction; however, EBT may still increase during COPD exacerbations. In recent studies, the change in EBT after cigarette smoking in individuals without a COPD diagnosis significantly predicted disease progression after 2 years. Early interventions based on these findings should be tested for efficacy in preventing the development of overt COPD. FAU - Vrbica, Zarko AU - Vrbica Z AUID- ORCID: 0000-0002-2208-7149 AD - Department for Health, University of Dubrovnik, 20000 Dubrovnik, Croatia. AD - Dubrovnik General Hospital, 20000 Dubrovnik, Croatia. FAU - Steiner, Justinija AU - Steiner J AD - Osijek-Baranja County Health Center, 31000 Osijek, Croatia. FAU - Plavec, Davor AU - Plavec D AUID- ORCID: 0000-0003-2020-8119 AD - Prima Nova, Healthcare Institution, 10090 Zagreb, Croatia. AD - Medical Faculty, University JJ Stossmayer, 31000 Osijek, Croatia. LA - eng PT - Journal Article PT - Review DEP - 20260922 PL - Switzerland TA - Medicina (Kaunas) JT - Medicina (Kaunas, Lithuania) JID - 9425208 SB - IM MH - Humans MH - *Pulmonary Disease, Chronic Obstructive/physiopathology/diagnosis MH - Breath Tests/methods MH - *Exhalation/physiology MH - Disease Progression MH - *Temperature PMC - PMC13707227 OTO - NOTNLM OT - COPD OT - aging OT - exhaled breath temperature OT - pre-COPD OT - smoking COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/09/22 CRDT- 2026/10/27 10:42 PHST- 2026/05/27 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - medicina62101823 [pii] AID - medicina-62-01823 [pii] AID - 10.3390/medicina62101823 [doi] PST - epublish SO - Medicina (Kaunas). 2026 Sep 22;62(10):1823. doi: 10.3390/medicina62101823. PMID- 42942347 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 21 TI - Right Place, Right Function: The Contribution of Subcellular Localization to the Physiological Impact of TRPM2 Channels. LID - 10.3390/life16101761 [doi] LID - 1761 AB - The channel TRPM2 forms cation-selective channels and is activated by redox-dynamics, including oxidative stress. Initially described at the plasma membrane, TRPM2 has been found to also localized to intracellular compartments, i.e., nucleus, mitochondria, lysosomes, and the endoplasmic reticulum. Its subcellular distribution appears to influence the Ca(2+) signals and signaling pathways associated with each compartment, resulting in distinct cellular outcomes. In cancer, TRPM2 has been implicated in proliferation, survival, autophagy, mitochondrial homeostasis, metabolism, inflammation, and cell death. Notably, TRPM2 can exert opposing effects depending on the cellular context, oxidative state, stimulus, and subcellular localization. These findings suggest that the functional diversity of TRPM2 may arise, at least in part, from its spatial organization within the cell. FAU - Hasselsweiller, Alice AU - Hasselsweiller A AUID- ORCID: 0009-0000-7475-5092 AD - Univ. Lille, Inserm, CHU Lille, CNRS, U1366-UMR9020-CRCLille-Cancer Research Center of Lille, F-59000 Lille, France. FAU - Nguyen, Bich-Thi Camille AU - Nguyen BC AUID- ORCID: 0009-0003-4413-1336 AD - Univ. Lille, Inserm, CHU Lille, CNRS, U1366-UMR9020-CRCLille-Cancer Research Center of Lille, F-59000 Lille, France. FAU - Soussi, Mayar AU - Soussi M AD - Univ. Lille, Inserm, CHU Lille, CNRS, U1366-UMR9020-CRCLille-Cancer Research Center of Lille, F-59000 Lille, France. FAU - Gkika, Dimitra AU - Gkika D AUID- ORCID: 0000-0001-8612-0836 AD - Univ. Lille, Inserm, CHU Lille, CNRS, U1366-UMR9020-CRCLille-Cancer Research Center of Lille, F-59000 Lille, France. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13707065 OTO - NOTNLM OT - TRPM2 OT - calcium signaling OT - intracellular calcium homeostasis OT - organelle function OT - oxidative stress OT - subcellular localization COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:42 PHST- 2026/09/21 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - life16101761 [pii] AID - life-16-01761 [pii] AID - 10.3390/life16101761 [doi] PST - epublish SO - Life (Basel). 2026 Oct 21;16(10):1761. doi: 10.3390/life16101761. PMID- 42942346 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 21 TI - Shifting High-Risk HPV Genotype Patterns in Histologically Confirmed Cervical HSIL: Temporal Changes and Differential Distribution in Invasive Squamous Cell Carcinoma. LID - 10.3390/life16101760 [doi] LID - 1760 AB - High-risk human papillomavirus (HR-HPV) genotypes differ in their oncogenic potential and contribution to cervical precancer and invasive cancer. We investigated temporal changes in HR-HPV genotype patterns in histologically confirmed cervical high-grade squamous intraepithelial lesions (HSIL) and differences between 2022-2025 HSIL and invasive squamous cell carcinoma (SCC) in an opportunistic screening setting in Spain. This retrospective study included 371 patients with HSIL from 2011 to 2016, 411 with HSIL from 2022 to 2025, and 65 with SCC from 2022 to 2025. Among HR-HPV-positive HSIL, HPV16/18 frequency decreased from 65.7% to 50.8% (p = 0.003), accompanied by a lower mean number of detected genotypes. After adjustment for age and cytological diagnosis, the 2022-2025 period was associated with lower odds of HPV16 (OR 0.59) and HPV18 (OR 0.36) detection. Compared with 2022-2025 HSIL, SCC showed higher HPV16/18 frequency (75.0% vs. 50.8%; p = 0.003), fewer multiple infections, and lower representation of other HR-HPV genotypes. Exploratory birth-cohort analysis showed lower HPV16/18 frequency in women born in or after 1994, with higher individual frequencies of HPV52 and HPV58. HR-HPV genotype patterns in histologically confirmed HSIL varied over time and between birth cohorts, while invasive SCC showed greater HPV16/18 representation and lower HPV diversity. FAU - Diaz Del Arco, Cristina AU - Diaz Del Arco C AUID- ORCID: 0000-0002-6100-4967 AD - Department of Pathology, Health Research Institute of the Hospital Clinico San Carlos (IdISSC), Hospital Clinico San Carlos, 28040 Madrid, Spain. AD - Department of Legal Medicine, Psychiatry and Pathology, School of Medicine, Complutense University of Madrid, 28040 Madrid, Spain. FAU - Ramirez Mena, Mar AU - Ramirez Mena M AD - Department of Obstetrics and Gynecology, Health Research Institute of the Hospital Clinico San Carlos (IdISSC), Hospital Clinico San Carlos, 28040 Madrid, Spain. FAU - Rojas Calles, Silvia AU - Rojas Calles S AD - School of Biological Sciences, Complutense University of Madrid, 28040 Madrid, Spain. FAU - Duran Botia, Fernando AU - Duran Botia F AD - Department of Pathology, Health Research Institute of the Hospital Clinico San Carlos (IdISSC), Hospital Clinico San Carlos, 28040 Madrid, Spain. FAU - Bellon Del Amo, Monica AU - Bellon Del Amo M AD - Department of Obstetrics and Gynecology, Health Research Institute of the Hospital Clinico San Carlos (IdISSC), Hospital Clinico San Carlos, 28040 Madrid, Spain. FAU - Garcia Martinez, Ricardo AU - Garcia Martinez R AUID- ORCID: 0000-0002-5418-4496 AD - Department of Pathology, Health Research Institute of the Hospital Clinico San Carlos (IdISSC), Hospital Clinico San Carlos, 28040 Madrid, Spain. FAU - Ortega Medina, Luis AU - Ortega Medina L AD - Department of Pathology, Health Research Institute of the Hospital Clinico San Carlos (IdISSC), Hospital Clinico San Carlos, 28040 Madrid, Spain. AD - Department of Legal Medicine, Psychiatry and Pathology, School of Medicine, Complutense University of Madrid, 28040 Madrid, Spain. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705950 OTO - NOTNLM OT - HPV epidemiology OT - HPV genotypes OT - cervical HSIL OT - cervical cancer OT - cervical squamous cell carcinoma OT - genotype distribution OT - human papillomavirus COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:42 PHST- 2026/09/12 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - life16101760 [pii] AID - life-16-01760 [pii] AID - 10.3390/life16101760 [doi] PST - epublish SO - Life (Basel). 2026 Oct 21;16(10):1760. doi: 10.3390/life16101760. PMID- 42942345 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 20 TI - Comparative Outcomes of Ultrasound-Guided Versus Fluoroscopy-Guided Percutaneous Transhepatic Biliary Drainage (PTBD) Due to Malignant Obstructive Jaundice: A Single-Center Retrospective Analysis. LID - 10.3390/life16101759 [doi] LID - 1759 AB - Background: Percutaneous transhepatic biliary drainage (PTBD) remains a cornerstone in managing obstructive jaundice, particularly when endoscopic retrograde cholangiopancreatography (ERCP) is unsuccessful or contraindicated. This study aims to compare ultrasound-guided (US-PTBD) and fluoroscopy-guided (F-PTBD) techniques regarding procedural time, radiation exposure, technical success, and complication rates. Methods: This retrospective, single-center study included patients with malignant obstructive jaundice who underwent PTBD between January 2018 and December 2025. Patients were stratified based on the imaging modality used for the bile duct puncture (US-PTBD vs. F-PTBD). Procedural characteristics, including dose-area product (DAP), fluoroscopy time, complication rates, and technical success, were analyzed using statistical methods appropriate for continuous and categorical data. Results: A total of 133 patients (163 PTBD procedures) were included. US-PTBD demonstrated significantly lower DAP (4.1 +/- 1.9 Gy.cm(2) vs. 6.2 +/- 1.5 Gy.cm(2); p < 0.001) and shorter procedure time (42 +/- 7 min vs. 56.6 +/- 6 min; p < 0.001) compared to F-PTBD. Both techniques had comparable technical success rates (91.1% vs. 82.2%; p = 0.158). Complications occurred in 10.2% of patients in the F-PTBD group and 6.7% in the US-PTBD group, with no statistically significant difference between groups (p = 0.489). Major complications, including bleeding and cholangitis, occurred exclusively in the F-PTBD cohort. US-PTBD resulted in fewer Glisson capsule punctures, suggesting reduced procedural trauma. Conclusions: Although a significant access side imbalance was present between cohorts, US-PTBD offers distinct advantages over F-PTBD, including reduced radiation exposure, shorter procedure times, and a comparable safety profile. These findings highlight the potential of US guidance as a safer alternative for PTBD, particularly in patients with coagulopathy or complex anatomy. Further multicenter studies with larger populations are warranted to confirm these benefits and refine patient selection criteria. FAU - Giurdanella, Marco AU - Giurdanella M AD - Radiology Unit, Dulbecco University Hospital, 88100 Catanzaro, Italy. FAU - Lagana, Domenico AU - Lagana D AUID- ORCID: 0000-0001-9158-0140 AD - Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro, 88100 Catanzaro, Italy. FAU - Battaglia, Caterina AU - Battaglia C AD - Radiology Unit, Dulbecco University Hospital, 88100 Catanzaro, Italy. FAU - Conti, Giuliana AU - Conti G AUID- ORCID: 0009-0006-8465-6323 AD - Radiology Unit, Dulbecco University Hospital, 88100 Catanzaro, Italy. FAU - Rosi, Nicola De AU - Rosi N AD - Radiology Unit, Dulbecco University Hospital, 88100 Catanzaro, Italy. FAU - Manti, Francesco AU - Manti F AD - Radiology Unit, Dulbecco University Hospital, 88100 Catanzaro, Italy. FAU - Mazzeo, Giuseppe AU - Mazzeo G AUID- ORCID: 0009-0003-9528-1115 AD - Radiology Unit, Dulbecco University Hospital, 88100 Catanzaro, Italy. FAU - Fulciniti, Marco AU - Fulciniti M AD - Radiology Unit, Dulbecco University Hospital, 88100 Catanzaro, Italy. FAU - Arcuri, Pier Paolo AU - Arcuri PP AUID- ORCID: 0000-0002-3992-8581 AD - Radiology Unit, Dulbecco University Hospital, 88100 Catanzaro, Italy. FAU - Maglia, Claudio AU - Maglia C AUID- ORCID: 0000-0001-6923-169X AD - Radiology Unit, Dulbecco University Hospital, 88100 Catanzaro, Italy. FAU - Tiralongo, Francesco AU - Tiralongo F AUID- ORCID: 0000-0001-9637-1970 AD - Radiology Unit 1, Department of Medical Surgical Sciences and Advanced Technologies "GF Ingrassia", University Hospital Policlinico "G. Rodolico-San Marco", University of Catania, 95123 Catania, Italy. FAU - Minici, Roberto AU - Minici R AUID- ORCID: 0000-0002-7680-0851 AD - Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro, 88100 Catanzaro, Italy. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13707143 OTO - NOTNLM OT - PTBD OT - PTC OT - biliary drainage OT - complications OT - fluoroscopy OT - jaundice OT - percutaneous OT - radiation exposure OT - transhepatic OT - ultrasound COIS- The authors declare no conflicts of interest. A preliminary version of this work was previously made available as a preprint: https://www.preprints.org/manuscript/202501.1603 (accessed on 1 October 2026). EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:42 PHST- 2026/01/20 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - life16101759 [pii] AID - life-16-01759 [pii] AID - 10.3390/life16101759 [doi] PST - epublish SO - Life (Basel). 2026 Oct 20;16(10):1759. doi: 10.3390/life16101759. PMID- 42942342 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 20 TI - Optimizing Drug-Coated Balloon Outcomes: The Evolving Role of Vessel Preparation in Coronary and Peripheral Artery Interventions. LID - 10.3390/life16101756 [doi] LID - 1756 AB - Drug-coated balloon (DCB) therapy differs fundamentally from stent-based revascularization since the vessel itself becomes the treatment substrate. Consequently, vascular preparation is an important component of DCB therapy. This review therefore examines contemporary vessel preparation before DCB therapy across coronary and peripheral interventions, integrating lesion morphology, procedural outcomes, intravascular imaging, and clinical follow-up. Across vascular beds, the objective is not maximal plaque disruption, but sufficient modification of the dominant mechanical limitation to achieve predictable expansion, controlled vessel injury, and effective balloon-vessel wall contact. Subsequent failure after coronary DCB therapy has been associated with residual plaque burden and calcium morphology. Specialty balloons, atherectomy and intravascular lithotripsy have been associated with improved procedural success, lumen expansion, and calcium modification, with lower rates of flow-limiting dissection or bailout stenting in selected settings. Randomized evidence has generally demonstrated more consistent procedural than long-term clinical benefits. Moreover, much of the evidence supporting advanced calcium-modification strategies derives from stent-based interventions and cannot be directly extrapolated to DCB-only therapy. These findings favor a lesion-specific, preparation-dependent approach rather than routine escalation to increasingly aggressive technologies. Conventional balloons may be sufficient for mechanically responsive lesions, whereas scoring or cutting balloons, atherectomy, or intravascular lithotripsy should be selected according to lesion morphology and the specific mechanical limitation that persists after initial preparation. Intravascular imaging may facilitate objective assessment of a "DCB-ready" vessel, although validated preparation thresholds remain undefined. The question is thus not which preparation device is preferable but whether the vessel has been adequately prepared to receive the drug. Future studies should go beyond device-to-device comparisons and assess whether predefined, imaging-based preparation targets improve drug delivery, vessel healing, and durable clinical outcomes. The next step toward precision DCB therapy across coronary and peripheral disease may be to shift the focus from device selection to treatment-substrate optimization. FAU - Malakouti, Sara AU - Malakouti S AUID- ORCID: 0009-0001-1141-9910 AD - DCB Academy, Cleveland, OH 44106, USA. AD - Cardiovascular Research Group, Fondazione Ricerca e Innovazione Cardiovascolare, 20143 Milano, Italy. FAU - Basavarajaiah, Sandeep AU - Basavarajaiah S AD - DCB Academy, Cleveland, OH 44106, USA. AD - Department of Cardiology, Heartlands Hospital, University Hospitals Birmingham NHS Foundation Trust, Birmingham B9 5SS, UK. FAU - Shah, Nihit AU - Shah N AD - Department of Cardiology, Heartlands Hospital, University Hospitals Birmingham NHS Foundation Trust, Birmingham B9 5SS, UK. FAU - Cortese, Bernardo AU - Cortese B AUID- ORCID: 0000-0002-5808-7810 AD - DCB Academy, Cleveland, OH 44106, USA. AD - Cardiovascular Research Group, Fondazione Ricerca e Innovazione Cardiovascolare, 20143 Milano, Italy. AD - Case Western Reserve University, Cleveland, OH 44106, USA. LA - eng PT - Journal Article PT - Review DEP - 20261020 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706622 OTO - NOTNLM OT - atherectomy OT - calcium modification OT - coronary intervention OT - drug-coated balloon OT - intravascular imaging OT - intravascular lithotripsy OT - leave-nothing-behind strategy OT - lesion modification OT - peripheral artery disease OT - vessel preparation COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:42 PHST- 2026/09/05 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - life16101756 [pii] AID - life-16-01756 [pii] AID - 10.3390/life16101756 [doi] PST - epublish SO - Life (Basel). 2026 Oct 20;16(10):1756. doi: 10.3390/life16101756. PMID- 42942339 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 19 TI - Procalcitonin in Acute Pancreatitis: Diagnostic Utility for Differentiating Infected from Sterile Pancreatic Necrosis. LID - 10.3390/life16101753 [doi] LID - 1753 AB - Acute pancreatitis (AP) is an acute inflammatory disorder of the pancreas that disrupts its normal function and often requires hospitalization because of severe abdominal pain. While most patients recover without major complications, some develop pancreatic necrosis, infected pancreatic necrosis (IPN), persistent organ failure, and other severe complications associated with increased morbidity and mortality. A key clinical challenge is distinguishing infected pancreatic necrosis from sterile pancreatic inflammation, as delayed or inaccurate diagnosis may result in inappropriate antibiotic use and poorer clinical outcomes. This review aimed to evaluate the diagnostic and prognostic utility of procalcitonin (PCT) in differentiating infected pancreatic necrosis from sterile inflammation and to assess its role in supporting antimicrobial decision-making in patients with AP. A structured narrative literature review was conducted using PubMed, Embase, Scopus, and Web of Science to identify English-language human studies, meta-analyses, randomized controlled trials, and international guidelines published between January 2000 and December 2025. A total of 60 studies were included in the narrative synthesis. The reviewed evidence indicates that PCT may provide greater diagnostic specificity for bacterial infection than conventional inflammatory markers such as C-reactive protein (CRP) and leukocyte count in selected clinical settings. Elevated or persistently increasing PCT concentrations have been associated with IPN, sepsis, organ failure, and increased mortality. Furthermore, PCT-guided antibiotic stewardship strategies may reduce unnecessary antibiotic exposure without adversely affecting clinical outcomes in selected settings. Overall, PCT represents a potentially valuable adjunctive biomarker for differentiating IPN from sterile inflammation in patients with AP. When interpreted alongside clinical assessment and imaging findings, PCT may facilitate the assessment of infection risk, prognostic stratification, and antimicrobial stewardship. However, further large-scale prospective multicenter studies are warranted to establish standardized diagnostic thresholds and optimize its integration into routine clinical practice. FAU - Kandasamy, Geetha AU - Kandasamy G AD - Department of Clinical Pharmacy, College of Pharmacy, King Khalid University, Abha 61421, Saudi Arabia. FAU - Selvaraj, Lavanya AU - Selvaraj L AUID- ORCID: 0000-0002-3655-5839 AD - Department of Pharmacy Practice, PSG College of Pharmacy, Peelamedu, Coimbatore 641004, Tamil Nadu, India. FAU - Ambrish Pathak, Yashvi AU - Ambrish Pathak Y AUID- ORCID: 0009-0002-2636-397X AD - Department of Pharmacy Practice, PSG College of Pharmacy, Peelamedu, Coimbatore 641004, Tamil Nadu, India. FAU - Anandan, Sangavi AU - Anandan S AUID- ORCID: 0009-0000-4107-360X AD - Department of Pharmacy Practice, PSG College of Pharmacy, Peelamedu, Coimbatore 641004, Tamil Nadu, India. FAU - Prasannan, Arun AU - Prasannan A AD - Department of Gastroenterology, GKNM Hospital, Nethaji Road, Pappanaickenpalayam, Coimbatore 641037, Tamil Nadu, India. FAU - Ahmed, Rehab AU - Ahmed R AUID- ORCID: 0000-0003-2476-469X AD - Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, University of Tabuk, Tabuk 71491, Saudi Arabia. FAU - Mani, Vasudevan AU - Mani V AUID- ORCID: 0000-0002-8440-8366 AD - Department of Pharmacology and Toxicology, College of Pharmacy, Qassim University, Buraydah 51452, Saudi Arabia. LA - eng GR - The authors extend their appreciation to the Deanship of Research and Graduate studies at King Khalid University for funding this work through large Research Project under grant number RGP2/45/47./Deanship of King Khalid University/ PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706023 OTO - NOTNLM OT - acute pancreatitis OT - antimicrobial stewardship OT - biomarkers OT - infection OT - pancreatitis OT - procalcitonin COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:42 PHST- 2026/08/29 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - life16101753 [pii] AID - life-16-01753 [pii] AID - 10.3390/life16101753 [doi] PST - epublish SO - Life (Basel). 2026 Oct 19;16(10):1753. doi: 10.3390/life16101753. PMID- 42942334 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 18 TI - Machine Learning-Based Screening for Obstructive Sleep Apnea Using Photographic Craniofacial and Speech-Derived Acoustic Features. LID - 10.3390/life16101748 [doi] LID - 1748 AB - Obstructive sleep apnea (OSA) is a prevalent disorder for which accessible non-invasive screening approaches remain of interest. This study evaluated photographic craniofacial descriptors and speech-derived acoustic features for Apnea-Hypopnea Index (AHI) estimation and binary OSA screening. Data from 109 adults were analyzed using unsegmented and temporally segmented speech representations. Seven craniofacial, acoustic, and combined feature configurations were evaluated using nested cross-validation. For regression, the lowest MAE was 14.242 events/h using segmented continuous-speech features with Random Forest. For classification, the highest AUC-ROC was 0.766 using unsegmented craniofacial features with a multilayer perceptron classifier, while the best segmented result was 0.756 using craniofacial plus continuous-speech features. Craniofacial-only features in the segmented representation achieved a similar AUC-ROC of 0.753, indicating no clear discriminative advantage from adding acoustic features. Several classifiers showed high sensitivity but comparatively low specificity. Sensitivity analysis excluding the four participants with the highest AHI values showed similar classification patterns, although regression performance was more sensitive to cohort composition. Overall, craniofacial descriptors provided the most consistent predictive contribution, whereas acoustic features showed model- and representation-dependent value for OSA screening. FAU - Lopez-Zaldivar, Marissa AU - Lopez-Zaldivar M AUID- ORCID: 0000-0002-6358-2226 AD - Universidad de Guadalajara, Centro Universitario de Ciencias Exactas e Ingenierias, Departamento de Bioingenieria Traslacional, Guadalajara 44430, Jalisco, Mexico. FAU - Torres-Ramos, Sulema AU - Torres-Ramos S AUID- ORCID: 0000-0002-2677-7155 AD - Universidad de Guadalajara, Centro Universitario de Ciencias Exactas e Ingenierias, Departamento de Ciencias Computacionales, Guadalajara 44430, Jalisco, Mexico. FAU - Roman-Godinez, Israel AU - Roman-Godinez I AUID- ORCID: 0000-0002-7404-8096 AD - Universidad de Guadalajara, Centro Universitario de Ciencias Exactas e Ingenierias, Departamento de Innovacion Basada en la Informacion y el Conocimiento, Guadalajara 44430, Jalisco, Mexico. FAU - Hernandez-Gordillo, Daniel AU - Hernandez-Gordillo D AUID- ORCID: 0000-0001-7754-4450 AD - UMAE Hospital de Especialidades, Centro Medico Nacional de Occidente, IMSS, Guadalajara 44340, Jalisco, Mexico. FAU - Vazquez-Jimenez, Aurora Sofia AU - Vazquez-Jimenez AS AUID- ORCID: 0000-0002-8846-3899 AD - UMAE Hospital de Especialidades, Centro Medico Nacional de Occidente, IMSS, Guadalajara 44340, Jalisco, Mexico. FAU - Salido-Ruiz, Ricardo Antonio AU - Salido-Ruiz RA AUID- ORCID: 0000-0001-6135-9306 AD - Universidad de Guadalajara, Centro Universitario de Ciencias Exactas e Ingenierias, Departamento de Bioingenieria Traslacional, Guadalajara 44430, Jalisco, Mexico. LA - eng PT - Journal Article DEP - 20261018 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705782 OTO - NOTNLM OT - acoustic features OT - apnea-hypopnea index OT - craniofacial descriptors OT - machine learning OT - obstructive sleep apnea OT - screening OT - speech analysis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:42 PHST- 2026/07/10 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - life16101748 [pii] AID - life-16-01748 [pii] AID - 10.3390/life16101748 [doi] PST - epublish SO - Life (Basel). 2026 Oct 18;16(10):1748. doi: 10.3390/life16101748. PMID- 42942322 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 16 TI - Vascular Rings as a Cause of Respiratory Difficulties. LID - 10.3390/life16101736 [doi] LID - 1736 AB - Background: Vascular rings are rare congenital developmental anomalies of the cardiovascular system that present with variable respiratory and gastrointestinal symptoms often refractory to what is considered a standard treatment. The aim of our study was to describe the clinical presentation, diagnostic pathway, and management of children referred to a tertiary pediatric department for unexplained respiratory symptoms in whom a vascular ring was confirmed, with particular attention to the role of echocardiography in raising the initial suspicion, and to compare our findings with published cohorts. Methods: We performed a retrospective descriptive case series of patients (n = 7) diagnosed at the Department of Pediatrics and Adolescent Medicine, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, University Hospital Martin, between 2021 and 2025. Results: The median age at onset of clinical symptoms was 30 days (interquartile range [IQR] 1-120 days). The median age at definitive diagnosis was 14.5 months (IQR 4.3-94.0 months). A genetic abnormality was observed in 3 patients (42.9%), and a positive family history of congenital heart defects was present in 4 (57.1%). All patients (n = 7) were symptomatic at the time of definitive diagnosis. We observed double aortic arch (DAA) in 2 patients (28.6%), right aortic arch with aberrant left subclavian artery (RAA + ALSA) in 4 (57.1%), and aberrant right subclavian artery (ARSA) in 1 (14.3%). One patient with RAA + ALSA had a concurrent pulmonary artery sling (PAS). In 6 patients (85.7%), the initial suspicion was raised by transthoracic or prenatal echocardiography. Six patients (85.7%) underwent surgical repair at a median age of 10.5 months (IQR 4.1-20.1 months), while in one case the risk-benefit ratio of surgery is being evaluated. Conclusions: In this series, echocardiography was a useful non-invasive modality for raising the initial suspicion, whereas CT angiography was required to define the vascular anatomy; the majority of patients were definitively diagnosed and treated within the first two years of life. In our cohort, we also identified a rare combination of a vascular ring (RAA + ALSA) and a pulmonary artery sling (PAS) in a single patient. FAU - Sulov, Jakub AU - Sulov J AD - Department of Pediatrics and Adolescent Medicine, University Hospital Martin, Kollarova 2, 036 59 Martin, Slovakia. AD - Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4A, 036 01 Martin, Slovakia. FAU - Oleksak, Filip AU - Oleksak F AD - Department of Pediatrics and Adolescent Medicine, University Hospital Martin, Kollarova 2, 036 59 Martin, Slovakia. AD - Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4A, 036 01 Martin, Slovakia. FAU - Vojtkova, Jarmila AU - Vojtkova J AUID- ORCID: 0000-0001-8666-1763 AD - Department of Pediatrics and Adolescent Medicine, University Hospital Martin, Kollarova 2, 036 59 Martin, Slovakia. AD - Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4A, 036 01 Martin, Slovakia. FAU - Balazsova, Pavlina AU - Balazsova P AD - Department of Pediatrics and Adolescent Medicine, University Hospital Martin, Kollarova 2, 036 59 Martin, Slovakia. FAU - Sorsakova, Alzbeta AU - Sorsakova A AD - Department of Pediatrics and Adolescent Medicine, University Hospital Martin, Kollarova 2, 036 59 Martin, Slovakia. AD - Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4A, 036 01 Martin, Slovakia. FAU - Petrovicova, Otilia AU - Petrovicova O AD - Department of Pediatrics and Adolescent Medicine, University Hospital Martin, Kollarova 2, 036 59 Martin, Slovakia. AD - Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4A, 036 01 Martin, Slovakia. FAU - Durdikova, Anna AU - Durdikova A AD - Department of Pediatrics and Adolescent Medicine, University Hospital Martin, Kollarova 2, 036 59 Martin, Slovakia. AD - Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4A, 036 01 Martin, Slovakia. FAU - Durdik, Peter AU - Durdik P AUID- ORCID: 0000-0002-5813-3039 AD - Department of Pediatrics and Adolescent Medicine, University Hospital Martin, Kollarova 2, 036 59 Martin, Slovakia. AD - Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4A, 036 01 Martin, Slovakia. FAU - Jesenak, Milos AU - Jesenak M AUID- ORCID: 0000-0001-7976-2523 AD - Department of Pediatrics and Adolescent Medicine, University Hospital Martin, Kollarova 2, 036 59 Martin, Slovakia. AD - Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4A, 036 01 Martin, Slovakia. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706560 OTO - NOTNLM OT - echocardiography OT - pediatric patients OT - respiratory difficulties OT - surgical repair OT - vascular rings COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:42 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - life16101736 [pii] AID - life-16-01736 [pii] AID - 10.3390/life16101736 [doi] PST - epublish SO - Life (Basel). 2026 Oct 16;16(10):1736. doi: 10.3390/life16101736. PMID- 42942319 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 15 TI - Explainable Ensemble Boosting Framework for Chronic Disease Risk Prediction Using Lifestyle and Clinical Data: A Multi-Dataset Study. LID - 10.3390/life16101734 [doi] LID - 1734 AB - Chronic diseases represent a substantial public health burden, motivating the development of transparent and reproducible approaches for disease-specific risk prediction using routinely available lifestyle and clinical variables. This study presents a common explainable ensemble boosting framework for comparative prediction across three independent disease-specific datasets: heart disease, lung cancer, and diabetes. Five boosting algorithms, including XGBoost, CatBoost, LightGBM, AdaBoost, and Gradient Boosting, were evaluated under a consistent leakage-free protocol. Each dataset was first split into stratified 80% training and 20% held-out test sets, with preprocessing, random oversampling, hyperparameter optimization, probability calibration, and threshold selection performed only on the training data. Performance was assessed using accuracy, balanced accuracy, precision, sensitivity, specificity, F1-score, ROC-AUC, PR-AUC, Brier score, and bootstrap confidence intervals. On the held-out test sets, AdaBoost achieved the highest balanced accuracy (68.07%) and ROC-AUC (71.71%) for heart disease; CatBoost achieved the highest balanced accuracy (92.59%) and ROC-AUC (96.30%) for lung cancer, although these estimates require caution because the lung-cancer test set contained only eight negative observations; and XGBoost achieved the highest balanced accuracy (77.02%), whereas Gradient Boosting achieved the highest ROC-AUC (82.43%) for diabetes. Repeated stratified cross-validation showed significant differences among the five algorithms for balanced accuracy (Friedman chi2=26.293, p<0.001), ROC-AUC (chi2=21.173, p<0.001), and F1-score (chi2=15.093, p=0.0045). Across these endpoints, Gradient Boosting achieved the best mean rank (1.98), followed by CatBoost (2.47), although no model was uniformly the highest-performing across all datasets and evaluation measures. SHAP and LIME analyses were additionally used to characterize global and local feature contributions to model predictions. The findings demonstrate the feasibility of a common explainable boosting workflow across heterogeneous disease-specific benchmark datasets while emphasizing that these results constitute internal benchmark evidence and require independent external and prospective validation before clinical application. FAU - Ganie, Shahid Mohammad AU - Ganie SM AUID- ORCID: 0000-0001-9925-0402 AD - Department of Health Information Management and Technology, College of Applied Medical Sciences, King Faisal University, Al-Ahsa 31982, Saudi Arabia. LA - eng GR - KFUXXXXXX/King Faisal University/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706192 OTO - NOTNLM OT - LIME OT - SHAP OT - boosting algorithms OT - chronic disease prediction OT - ensemble learning OT - explainable artificial intelligence OT - probability calibration OT - repeated cross-validation COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:42 PHST- 2026/08/18 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - life16101734 [pii] AID - life-16-01734 [pii] AID - 10.3390/life16101734 [doi] PST - epublish SO - Life (Basel). 2026 Oct 15;16(10):1734. doi: 10.3390/life16101734. PMID- 42942318 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 15 TI - Domain-Aware and Multi-Scale Deep Learning Framework for Sperm Morphology Classification. LID - 10.3390/life16101732 [doi] LID - 1732 AB - Automated sperm morphology classification can reduce the subjectivity and workload of manual microscopic assessment, but limited benchmark data and subtle morphology-related visual differences remain important challenges. This study proposes a domain-specific ConvNeXt-Tiny-based framework for sperm morphology classification on the SMIDS benchmark. The framework combines Hi-LabSpermMorpho-based domain-specific pretraining, multi-scale feature extraction, attention-guided feature refinement, class-balanced sampling, test-time augmentation, MixUp, label smoothing, focal-loss-based optimization, and supervised contrastive learning. Domain-specific pretraining was compared with ImageNet initialization, and the framework was examined through cumulative ablation, component-removal experiments, and matched-condition architecture comparisons. The best configuration, combining Hi-LabSpermMorpho pretraining with 10x augmented training data, achieved a patch-level average accuracy of 92.73%, numerically the highest among the evaluated configurations and backbones. In matched-condition architecture comparisons, the differences between the proposed framework and EfficientNetV2-S, Swin-T, and domain-pretrained BEiT-Base were not statistically significant. The full training configuration significantly outperformed the baseline configuration under 5x augmentation, whereas the component-removal experiments did not establish the necessity of each individual component. Because the held-out test folds were also used for checkpoint selection, the reported accuracies are subject to selection bias and cannot be regarded as independent test estimates; accordingly, all comparisons should be regarded as exploratory. These findings suggest that domain-specific pretraining can support sperm morphology classification, although independent validation is required. FAU - Gul, Imran AU - Gul I AUID- ORCID: 0009-0006-9629-3814 AD - Department of Computer Engineering, Yildiz Technical University, Istanbul 34220, Turkiye. FAU - Ilhan, Hamza Osman AU - Ilhan HO AUID- ORCID: 0000-0002-1753-2703 AD - Department of Computer Engineering, Yildiz Technical University, Istanbul 34220, Turkiye. FAU - Uzun, Hakki AU - Uzun H AUID- ORCID: 0000-0002-5189-3166 AD - Department of Urology, Faculty of Medicine, Recep Tayyip Erdogan University, Rize 53020, Turkiye. FAU - Yigit, Merve Huner AU - Yigit MH AUID- ORCID: 0000-0001-8988-4321 AD - Department of Biochemistry, Faculty of Medicine, Recep Tayyip Erdogan University, Rize 53020, Turkiye. FAU - Serbes, Gorkem AU - Serbes G AUID- ORCID: 0000-0003-4591-7368 AD - Department of Biomedical Engineering, Yildiz Technical University, Istanbul 34220, Turkiye. LA - eng GR - 020260080020551/Recep Tayyip Erdogan University/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706106 OTO - NOTNLM OT - ConvNeXt OT - domain-specific pretraining OT - sperm morphology classification OT - supervised contrastive learning COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:42 PHST- 2026/08/25 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - life16101732 [pii] AID - life-16-01732 [pii] AID - 10.3390/life16101732 [doi] PST - epublish SO - Life (Basel). 2026 Oct 15;16(10):1732. doi: 10.3390/life16101732. PMID- 42942315 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 15 TI - Identification of Transcriptional Signatures Associated with COPD in Immune and Epithelial-Stromal Cells Using Machine Learning Methods. LID - 10.3390/life16101729 [doi] LID - 1729 AB - In the context of chronic obstructive pulmonary disease (COPD), understanding gene expression patterns across various cell types is pivotal for disease comprehension, therapeutic development, and enhancement of patient prognosis. Nevertheless, the intricate interplay between immune and structural cells, along with their significance in COPD pathogenesis, remains only partially understood. This research employed multiple machine learning techniques to pinpoint marker genes exhibiting pronounced alterations between COPD patients and healthy individuals. We utilized six feature selection algorithms (LASSO, LightGBM, MCFS, RF, CATboost, and XGBoost) and three classification methods (DT, KNN, and RF) to analyze single-cell patient data. Cells were bifurcated into two subsets: immune cells and epithelial-stromal cells. Each cell, encompassing 21,237 genes, was labeled either as a healthy control or COPD. We identified a number of promising genes, such as FKBP5, which encodes a protein crucial for stress response regulation, and SCGB3A1, which is predominantly expressed in the respiratory tract and critical for regulating lung function. Both genes exhibit strong correlations with COPD. The study also underscored the potential roles of HLA-DRB1 and HSP90AA1 in COPD pathogenesis. Our findings underscore the unparalleled potential of machine learning in identifying genetic markers for intricate diseases like COPD, paving the way for targeted therapeutic advancements. FAU - Ren, Jingxin AU - Ren J AD - School of Life Sciences, Shanghai University, Shanghai 200444, China. FAU - Fan, Jingjing AU - Fan J AD - Department of Radiotherapy, The Ninth Medical Center, Chinese PLA General Hospital, Beijing 100853, China. FAU - Bao, Yusheng AU - Bao Y AD - School of Life Sciences, Shanghai University, Shanghai 200444, China. FAU - Guo, Wei AU - Guo W AD - Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China. FAU - Feng, Kaiyan AU - Feng K AD - Department of Computer Science, Guangdong AIB Polytechnic College, Guangzhou 510507, China. FAU - Hu, Jerry AU - Hu J AD - Department of Natural Sciences and Mathematics, College of Natural and Applied Sciences, Texas A&M University-Victoria, Victoria, TX 77901, USA. FAU - Zhou, Wenran AU - Zhou W AUID- ORCID: 0009-0005-6828-4999 AD - School of Life Sciences, Shanghai University, Shanghai 200444, China. FAU - Wang, Deling AU - Wang D AD - State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat-Sen University Cancer Center, Guangzhou 510060, China. FAU - Lu, Lin AU - Lu L AD - Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA. FAU - Huang, Tao AU - Huang T AUID- ORCID: 0000-0003-1975-9693 AD - Bio-Med Big Data Center, CAS Key Laboratory of Computational Biology, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China. AD - Department of Artificial Intelligence and Digital Health, CAS Engineering Laboratory for Nutrition, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai 200031, China. FAU - Cai, Yu-Dong AU - Cai YD AUID- ORCID: 0000-0001-5664-7979 AD - School of Life Sciences, Shanghai University, Shanghai 200444, China. LA - eng GR - GZNL2024A01003/Major Project of Guangzhou National Laboratory/ GR - 202002/Key Laboratory of Tissue Microenvironment and Tumor of Chinese Academy of Sciences/ GR - ZR2022MC072/Shandong Provincial Natural Science Foundation/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706307 OTO - NOTNLM OT - chronic obstructive pulmonary disease OT - feature selection OT - gene expression OT - machine learning COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:42 PHST- 2026/08/16 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - life16101729 [pii] AID - life-16-01729 [pii] AID - 10.3390/life16101729 [doi] PST - epublish SO - Life (Basel). 2026 Oct 15;16(10):1729. doi: 10.3390/life16101729. PMID- 42942306 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 15 TI - Limb Salvage After Failed Infected Total Knee Arthroplasty: A Comparison of Cement-Based Artificial Arthrodesis and External Fixator Arthrodesis. LID - 10.3390/life16101721 [doi] LID - 1721 AB - Background: Persistent or recurrent periprosthetic joint infection after total knee arthroplasty is a devastating complication. When revision fails, knee arthrodesis remains an important limb-salvage option. Cement-based artificial arthrodesis may provide an alternative when extensive bone loss or soft-tissue compromise limits conventional fusion, but comparative evidence is scarce. Methods: This retrospective exploratory cohort included 36 patients whose index salvage procedures were performed between January 2014 and December 2023 after failed treatment of periprosthetic knee infection. Sixteen patients underwent external fixator arthrodesis, and 20 underwent cement-based artificial arthrodesis. Postoperative SF-12 and SF-36 scores, ambulation, limb shortening, extensor mechanism status, microbiological findings, reinfection, and complications requiring surgery were evaluated. Results: The groups differed at baseline: preoperative ESR and CRP were higher in the cement-based group, whereas extensor mechanism insufficiency was more frequent in the external fixator group (81.3% vs. 20%; p = 0.001). No statistically significant between-group differences were detected in most SF-12 and SF-36 domains; Energy/Fatigue was higher in the external fixator group (p = 0.011), an isolated exploratory finding. Mean postoperative shortening was numerically greater after external fixation (4.94 +/- 3.66 vs. 3.15 +/- 0.49 cm; p = 0.07). Complications requiring surgical intervention occurred in 31.2% and 25% of patients, respectively. Reinfection requiring additional surgery occurred in 0/16 external fixator patients and 1/20 cement-based patients. Conclusions: Cement-based artificial arthrodesis may represent a practical limb salvage option in selected patients with failed infected TKA, particularly when direct bone apposition is difficult. Because treatment allocation was non-random, baseline characteristics were imbalanced, and the cohort was small, so the findings should be interpreted as exploratory and not as evidence of equivalence or superiority. FAU - Camur, Savas AU - Camur S AUID- ORCID: 0000-0001-5899-6910 AD - Department of Orthopaedics and Traumatology, Umraniye Training and Research Hospital, 34764 Istanbul, Turkiye. FAU - Birinci, Murat AU - Birinci M AUID- ORCID: 0000-0002-6268-9910 AD - Department of Orthopaedics and Traumatology, Umraniye Training and Research Hospital, 34764 Istanbul, Turkiye. FAU - Bayram, Serkan AU - Bayram S AUID- ORCID: 0000-0001-7651-1200 AD - Department of Orthopaedics and Traumatology, Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkiye. FAU - Kemah, Bahattin AU - Kemah B AUID- ORCID: 0000-0002-4795-4309 AD - Department of Orthopaedics and Traumatology, Umraniye Training and Research Hospital, 34764 Istanbul, Turkiye. FAU - Karacam, Muhammet Ibrahim AU - Karacam MI AD - Department of Orthopaedics and Traumatology, Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkiye. FAU - Polat, Omer AU - Polat O AUID- ORCID: 0000-0002-6847-3555 AD - Department of Orthopaedics and Traumatology, Umraniye Training and Research Hospital, 34764 Istanbul, Turkiye. FAU - Ozcan, Cagri AU - Ozcan C AUID- ORCID: 0000-0002-6651-3322 AD - Department of Orthopaedics and Traumatology, Umraniye Training and Research Hospital, 34764 Istanbul, Turkiye. FAU - Balci, Halil Ibrahim AU - Balci HI AD - Department of Orthopaedics and Traumatology, Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkiye. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706599 OTO - NOTNLM OT - antibiotic-loaded cement OT - functional outcomes OT - knee fusion OT - limb length discrepancy OT - periprosthetic joint infection COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:42 PHST- 2026/09/12 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - life16101721 [pii] AID - life-16-01721 [pii] AID - 10.3390/life16101721 [doi] PST - epublish SO - Life (Basel). 2026 Oct 15;16(10):1721. doi: 10.3390/life16101721. PMID- 42942304 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 14 TI - The Unresolved Paradox of Viable Microorganisms in Ancient Halite. LID - 10.3390/life16101718 [doi] LID - 1718 AB - Reports of viable microorganisms from ancient halite create an unusual test of the biological limits of cellular survival. Halite deposits can be tens to hundreds of millions of years old, and increasingly rigorous studies have used chemical surface sterilization, petrographic selection of primary crystals, and direct sampling of primary fluid inclusions to reduce the likelihood of modern contamination. Nevertheless, the geological age of a crystal or inclusion does not by itself establish the age of a recovered organism. The central problem is that both major interpretations remain difficult. Modern contamination becomes less satisfactory when microorganisms are recovered from deeply buried, carefully sterilized primary material, yet uninterrupted survival for geological intervals is difficult to reconcile with spontaneous DNA and protein damage, ionizing radiation, finite energy supplies, and observed loss of microbial viability in other long-term environments. A further problem is molecular: several putatively ancient isolates or sequences are remarkably similar to modern organisms despite host-rock ages of tens to hundreds of millions of years. Proposed explanations include contamination, later recrystallization or groundwater infiltration, extremely low-activity maintenance states, and underestimation of genomic divergence by conserved markers such as 16S rRNA. No single explanation currently accounts for all reported observations and their heterogeneity in evidentiary quality. Ancient halite therefore provides a natural extreme-case model for a broader biomedical question: how long can a cell preserve sufficient molecular integrity for recovery when metabolism, repair, and replacement of damaged components approach their lower limits? FAU - Renshaw, Colby AU - Renshaw C AD - College of Osteopathic Medicine, Liberty University, Lynchburg, VA 24515, USA. FAU - Liu, Yingguang AU - Liu Y AUID- ORCID: 0000-0002-2114-3997 AD - College of Osteopathic Medicine, Liberty University, Lynchburg, VA 24515, USA. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706180 OTO - NOTNLM OT - DNA damage OT - cellular dormancy OT - fluid inclusions OT - halite OT - halophiles OT - microbial longevity OT - molecular repair COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:42 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - life16101718 [pii] AID - life-16-01718 [pii] AID - 10.3390/life16101718 [doi] PST - epublish SO - Life (Basel). 2026 Oct 14;16(10):1718. doi: 10.3390/life16101718. PMID- 42942302 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 14 TI - BIMS-GA: Adaptive Multi-Objective Optimization for Network-Aware Pancreatic Ductal Adenocarcinoma Biomarker Discovery. LID - 10.3390/life16101716 [doi] LID - 1716 AB - Pancreatic ductal adenocarcinoma (PDAC) has a five-year relative survival of 8%. Feature selection optimizes accuracy, survival concordance, and network structure separately; whether uniting them matters has not been determined. The BIMS-GA optimizes the cross-validated AUC, Cox concordance, and STRING v11.0 protein-protein interaction coherence in one fitness function and was compared against fourteen alternative methods on GSE62452 (n = 130) and eight external evaluation sets. Using a nested protocol, the fifteen methods had pooled out-of-fold AUCs spanning 0.877-0.900; the BIMS-GA ranked thirteenth with 0.8855. No DeLong comparisons survived correction. Stability did not persist either: the ten-seed Jaccard index of 0.804 exceeded every comparator but fell to 0.521 in a second seed block. Network coherence separated them by two orders of magnitude, 0.480 against 0.005-0.192, in an ordering unbroken across twenty runs. Removing the network term raised the discovery AUC by 0.0117 and lowered external prognostic concordance by 0.0149 in all ten runs, acting as a regularizer. The twenty-gene panel comprises one laminin-332 and an integrin adhesion program with no isolated member, stratifying survival in four cohorts (hazard ratios of 2.92-4.13 fitted within each cohort; 1.02-2.17 transferred). Network coherence, not discrimination, is the axis that remained separate in every run examined, while short-run stability did not. FAU - Saeheaw, Teerapun AU - Saeheaw T AUID- ORCID: 0000-0002-1956-2527 AD - Department of Teacher Training in Mechanical Engineering, King Mongkut's University of Technology North Bangkok, Bangkok 10800, Thailand. LA - eng GR - KMUTNB-FF-69-B-59/National Science, Research and Innovation Fund (NSRF), and King Mongkut's University of Technology North Bangkok/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705738 OTO - NOTNLM OT - biomarker discovery OT - feature selection OT - multi-objective genetic algorithm OT - pancreatic ductal adenocarcinoma OT - protein-protein interaction network COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:42 PHST- 2026/07/10 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - life16101716 [pii] AID - life-16-01716 [pii] AID - 10.3390/life16101716 [doi] PST - epublish SO - Life (Basel). 2026 Oct 14;16(10):1716. doi: 10.3390/life16101716. PMID- 42942301 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 14 TI - Bridging the Allergy-Radiology Gap in Radiocontrast Media Hypersensitivity: A Patient-Level Linkage Study on Diagnostic Stewardship and Re-Exposure Outcomes. LID - 10.3390/life16101715 [doi] LID - 1715 AB - Background/Objectives: We examined how allergy findings related to candidate-agent identification, subsequent real-world imaging and clinical outcomes. Methods: This single-centre retrospective cohort included 29 adults evaluated between January 2019 and May 2025 for contrast media hypersensitivity. Imaging and outcomes between allergy evaluation and a structured telephone interview in July 2026 were assessed retrospectively and verified in national electronic health records, without prospective follow-up. Primary outcomes were skin test positivity and new hypersensitivity reactions during subsequent contrast administration. Results: The culprit contrast agent was not documented by name in 18 patients (62.1%); median evaluation delay was 60 months. Skin tests were positive in 1/20 patients (5.0%); skin-test-negative candidate agents were identified in 19/20 (95.0%). New reactions occurred in 3/14 contrast recipients (21.4%), all with immediate Brown Grade III index reactions to iohexol. Subsequent reactions included one cardiac arrest after iohexol and two Grade II reactions after iodixanol. Of seven patients imaged without contrast, four had candidate agents but reported continuing patient and physician concerns. No Holm-adjusted comparison was significant. Conclusions: Skin testing identified a candidate agent in most tested patients, but candidate identification did not establish clinical tolerance or independent protection. A shared, individualised imaging plan should communicate agent selection, premedication decisions, test limitations and precautions to patients and radiologists while preserving access to necessary imaging. FAU - Toksoy Senturk, Meryem Irem AU - Toksoy Senturk MI AD - Division of Immunology and Allergy, Department of Internal Medicine, Faculty of Medicine, Ege University, 35100 Izmir, Turkiye. FAU - Dalgic, Ceyda Tunakan AU - Dalgic CT AD - Division of Immunology and Allergy, Department of Internal Medicine, Faculty of Medicine, Ege University, 35100 Izmir, Turkiye. FAU - Okan, Kasim AU - Okan K AD - Division of Immunology and Allergy, Department of Internal Medicine, Faculty of Medicine, Ege University, 35100 Izmir, Turkiye. FAU - Kural, Ragip Fatih AU - Kural RF AUID- ORCID: 0000-0001-5690-4090 AD - Division of Immunology and Allergy, Department of Internal Medicine, Faculty of Medicine, Ege University, 35100 Izmir, Turkiye. FAU - Galata, Zuleyha AU - Galata Z AUID- ORCID: 0000-0001-9627-4281 AD - Division of Immunology and Allergy, Department of Internal Medicine, Faculty of Medicine, Ege University, 35100 Izmir, Turkiye. FAU - Kirdok, Kutay AU - Kirdok K AUID- ORCID: 0000-0001-6613-6830 AD - Division of Immunology and Allergy, Department of Internal Medicine, Faculty of Medicine, Ege University, 35100 Izmir, Turkiye. FAU - Aslan, Eda AU - Aslan E AD - Division of Immunology and Allergy, Department of Internal Medicine, Faculty of Medicine, Ege University, 35100 Izmir, Turkiye. FAU - Canbaz, Turkan Dizdar AU - Canbaz TD AUID- ORCID: 0000-0001-6181-951X AD - Division of Immunology and Allergy, Department of Internal Medicine, Faculty of Medicine, Ege University, 35100 Izmir, Turkiye. FAU - Ates, Umitcan AU - Ates U AUID- ORCID: 0000-0002-6321-7317 AD - Division of Immunology and Allergy, Department of Internal Medicine, Faculty of Medicine, Ege University, 35100 Izmir, Turkiye. FAU - Ay, Ecem AU - Ay E AUID- ORCID: 0009-0006-0613-2823 AD - Division of Immunology and Allergy, Department of Internal Medicine, Faculty of Medicine, Ege University, 35100 Izmir, Turkiye. FAU - Yetemen, Seda Karaaslan AU - Yetemen SK AD - Division of Immunology and Allergy, Department of Internal Medicine, Faculty of Medicine, Ege University, 35100 Izmir, Turkiye. FAU - Gumusburun, Reyhan AU - Gumusburun R AUID- ORCID: 0000-0002-0274-5917 AD - Division of Immunology and Allergy, Department of Internal Medicine, Faculty of Medicine, Ege University, 35100 Izmir, Turkiye. FAU - Gokmen, Emine Nihal Mete AU - Gokmen ENM AD - Division of Immunology and Allergy, Department of Internal Medicine, Faculty of Medicine, Ege University, 35100 Izmir, Turkiye. FAU - Sin, Aytul Zerrin AU - Sin AZ AUID- ORCID: 0000-0002-9199-1991 AD - Division of Immunology and Allergy, Department of Internal Medicine, Faculty of Medicine, Ege University, 35100 Izmir, Turkiye. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706518 OTO - NOTNLM OT - contrast media hypersensitivity OT - contrast-enhanced imaging OT - gadolinium-based contrast agents OT - iodinated contrast media OT - premedication OT - retrospective cohort OT - skin testing OT - subsequent administration COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:42 PHST- 2026/09/22 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - life16101715 [pii] AID - life-16-01715 [pii] AID - 10.3390/life16101715 [doi] PST - epublish SO - Life (Basel). 2026 Oct 14;16(10):1715. doi: 10.3390/life16101715. PMID- 42942299 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 13 TI - Beyond Cartilage Grafting in Rhinoplasty: Biopharmaceutical and Biomaterial Strategies for Nasal Cartilage Regeneration. LID - 10.3390/life16101713 [doi] LID - 1713 AB - Autologous septal, auricular, and costal cartilage remain the reference materials for structural and aesthetic rhinoplasty, yet each source is constrained by finite availability, donor-site morbidity, variable geometry, and the risks of warping, resorption, or revision. Nasal cartilage regeneration therefore represents more than a conventional tissue-engineering problem: a clinically useful product must recreate region-specific extracellular matrix, survive in a diffusion-limited environment, tolerate suturing and cyclic deformation, preserve the nasal airway, and maintain patient-specific contour under a thin soft-tissue envelope. This narrative review evaluates next-generation biopharmaceutical and biomaterial strategies for meeting those requirements. Direct evidence from human nasal chondrocytes and engineered nasal constructs is integrated with mechanistic evidence from broader cartilage regeneration, with explicit separation of nasal, auricular, and articular data. Chondrogenic proteins and small molecules, including transforming growth factor-beta, bone morphogenetic proteins, fibroblast growth factor 18, and kartogenin, can initiate matrix-forming programs but require spatial and temporal control to avoid fibrocartilage, hypertrophy, or mineralization. Polydeoxyribonucleotide and polynucleotide formulations have plausible anti-inflammatory and matrix-supportive actions, but evidence remains predominantly in vitro or articular; no direct clinical proof currently supports injection-only regeneration of structural nasal cartilage. Extracellular vesicles provide a cell-free signaling platform, whereas expanded nasal chondrocytes presently have the strongest tissue-specific translational lineage. Injectable collagen, fibrin, alginate, hyaluronic-acid, chitosan, gelatin-methacryloyl, and decellularized-matrix systems can localize these cargos, although biologic permissiveness often conflicts with immediate mechanical strength. Three-dimensional bioprinting and reinforced hybrid constructs offer patient-specific geometry, but clinical translation requires validated potency assays, suture retention, flexural testing, orthotopic safety, Good Manufacturing Practice-compatible production, and long-term objective assessment of shape and airflow. The most credible near-term applications are biologic enhancement of existing grafts, repair of small contained defects, and patient-specific partial-framework replacement rather than minimally invasive reshaping by regenerative injection alone. A stage-gated translational framework is proposed to align molecular potency, material performance, surgical usability, aesthetic precision, and durable nasal function. FAU - Yi, Kyu-Ho AU - Yi KH AUID- ORCID: 0000-0001-5572-1364 AD - You and I Clinic, Seoul 06123, Republic of Korea. FAU - Lee, Han Earl AU - Lee HE AD - Opening Plastic Surgery Clinic, Seoul 06038, Republic of Korea. FAU - Kim, Hongseok AU - Kim H AUID- ORCID: 0009-0000-1156-7393 AD - VOS Dermatology Clinic, 1-4F, 806 Eonju-ro, Gangnam-gu, Seoul 06022, Republic of Korea. FAU - Choi, Wonseok AU - Choi W AD - V Plastic Surgery, Daegu 41940, Republic of Korea. FAU - Kim, Jin Hyun AU - Kim JH AD - You and I Clinic, Seoul 06123, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706184 OTO - NOTNLM OT - 3D bioprinting OT - aesthetic medicine OT - biopharmaceuticals OT - controlled release OT - extracellular vesicles OT - injectable hydrogel OT - nasal cartilage regeneration OT - polydeoxyribonucleotide OT - polynucleotides OT - rhinoplasty OT - tissue engineering COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:42 PHST- 2026/09/19 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - life16101713 [pii] AID - life-16-01713 [pii] AID - 10.3390/life16101713 [doi] PST - epublish SO - Life (Basel). 2026 Oct 13;16(10):1713. doi: 10.3390/life16101713. PMID- 42942292 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 12 TI - Preservation of Auxetic Oscillatory Capacity in Living Connective Tissue: An ECM-Regulated Hypothesis of Mechanical Homeostasis. LID - 10.3390/life16101706 [doi] LID - 1706 AB - Living connective tissue preserves function while adapting to changing loads. This hypothesis proposes that preserving access to reversible auxetic deformation can contribute to mechanical homeostasis in selected tissues. Auxetic oscillation (AO) denotes repeated, reversible expression of negative-Poisson behavior; it does not require transitions between Poisson regimes. AO range is defined as the longitudinal-strain interval over which a directional incremental Poisson measure is reliably negative, with loading, unloading, recovery, and cycle stability described separately. The proposed biological contribution is that broader recoverable AO exposure produces cell-matrix inputs that better preserve extracellular-matrix (ECM) adaptability than an appropriately varied non-auxetic comparison. A bounded annulus-fibrosus example predicts less persistent matrix compaction after repeated AO exposure and defined recovery, as a candidate structural indicator of preservation. Equivalent or worse matrix outcomes would count against that advantage. Biotensegrity supplies a candidate architectural rationale for distributed load management, while established tissue mechanics provide the experimental basis. Evidence that auxeticity occurs, that ECM state regulates it, and that preserving it benefits the matrix represents separate levels of support. The contribution is therefore a testable preservation hypothesis, rather than the discovery of a new deformation phenomenon. FAU - Murer, Brian R AU - Murer BR AUID- ORCID: 0009-0005-3017-4081 AD - Lazy 8 Healthcare and Consulting, P.C., Bloomington, IN 47401, USA. LA - eng PT - Journal Article DEP - 20261012 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705866 OTO - NOTNLM OT - Poisson ratio OT - auxetic oscillation OT - auxeticity OT - biotensegrity OT - deformation capacity OT - extracellular matrix OT - fascia OT - mechanical homeostasis OT - mechanotransduction COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:42 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - life16101706 [pii] AID - life-16-01706 [pii] AID - 10.3390/life16101706 [doi] PST - epublish SO - Life (Basel). 2026 Oct 12;16(10):1706. doi: 10.3390/life16101706. PMID- 42942287 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 10 TI - Alactic Base Excess as a Predictor of Mortality in Trauma ICU Patients: Retrospective Study and Evaluation of Integration into Trauma Scoring Systems. LID - 10.3390/life16101701 [doi] LID - 1701 AB - Risk stratification in severe trauma remains a major clinical challenge: standard trauma scores (Injury Severity Score, ISS; Trauma and Injury Severity Score, TRISS) rely on anatomical and physiological data but lack metabolic components, potentially underestimating risk in haemodynamically compensated patients with underlying derangements. Alactic base excess (ABE) isolates the non-lactic fraction of metabolic acidosis by correcting base excess for lactate. We conducted a retrospective cohort study using the MIMIC-IV v3.1 database, including adult trauma patients with an ISS >/= 16 and an arterial blood gas measurement within two hours of admission (n = 134), to assess ABE's independent and incremental prognostic value for in-hospital and 30-day mortality, handling missing covariates by multiple imputation (m = 20). ABE was independently associated with in-hospital mortality after adjustment for age, New ISS (NISS), and mechanism of injury (odds ratio 0.84; 95% CI 0.745-0.946; p = 0.004; AUC 0.643), but its prognostic content overlapped substantially with lactate, and its incremental value beyond a clinical base model and beyond TRISS was small and statistically fragile; decision curve analysis showed no consistent net benefit from adding ABE to TRISS. The findings were consistent for 30-day mortality and stronger in a 60 min sensitivity cohort (n = 54; AUC 0.803), though this small subgroup may reflect selection effects. ABE should not be regarded as evidence of a novel physiological pathway in this trauma cohort; its practical contribution, if any, lies in immediate availability from a routine blood gas before ISS or TRISS can typically be computed. These findings are hypothesis-generating, given the single-centre design and absence of external validation, and require prospective, multicentre confirmation before clinical application. FAU - Schollhorn, Felix Julian AU - Schollhorn FJ AUID- ORCID: 0009-0004-1704-4090 AD - Faculty of Medicine, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142 Targu Mures, Romania. FAU - Azamfirei, Leonard AU - Azamfirei L AUID- ORCID: 0000-0003-3220-2267 AD - Department of Anaesthesiology and Intensive Care, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142 Targu Mures, Romania. FAU - Branea, Oana Elena AU - Branea OE AUID- ORCID: 0009-0009-7616-3424 AD - Department of Anaesthesiology and Intensive Care, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142 Targu Mures, Romania. LA - eng PT - Journal Article DEP - 20261010 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706226 OTO - NOTNLM OT - alactic base excess OT - intensive care OT - metabolic acidosis OT - mortality prediction OT - risk stratification COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:42 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - life16101701 [pii] AID - life-16-01701 [pii] AID - 10.3390/life16101701 [doi] PST - epublish SO - Life (Basel). 2026 Oct 10;16(10):1701. doi: 10.3390/life16101701. PMID- 42942286 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 10 TI - Spent Mushroom Substrate Supplemented with Streptomyces gardneri MC1-2 Improves Tomato Seedling Growth and Its Establishment in Soil Application. LID - 10.3390/life16101699 [doi] LID - 1699 AB - Spent mushroom substrate (SMS), a lignocellulosic by-product of mushroom cultivation, represents a promising renewable carrier for microbial bioinoculants as well as agricultural waste valorization. This study evaluated SMS as a carrier for the plant growth-promoting actinobacterium Streptomyces gardneri MC1-2 and assessed its effects on tomato growth and soil bacterial communities. Three formulations were prepared: SMS alone (F1), SMS supplemented with 1% (w/w) carboxymethyl cellulose (CMC) (F2), and talc supplemented with 1% CMC and 1.5% CaCO(3) (F3). S. gardneri MC1-2 exhibited in vitro traits commonly associated with plant-beneficial activity, including indolic compound production, siderophore production, and inorganic phosphate solubilization. During 30 days of storage at 4, 30, and 40 degrees C, both SMS-based formulations maintained viable populations at a 3 Log CFU reduction, whereas the talc-based formulation lost viability within seven days. Among the formulations, F2 showed the best overall performance and was selected for the pot experiment. Direct inoculation with S. gardneri MC1-2 promoted tomato growth comparably to chemical fertilizer, while F2 strongly supported growth in sterile soil, where S. gardneri MC1-2 established and dominated the bacterial community. In non-sterile soil, reduced bacterial establishment suggested that competition with native microbiota limited inoculant performance. These findings demonstrate that SMS can serve as an effective carrier for Streptomyces-based bioinoculants with potential applications for sustainable crop production. FAU - Kitwetch, Benyapa AU - Kitwetch B AD - Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. FAU - Mukjang, Nilita AU - Mukjang N AD - Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. AD - Center of Excellence in Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. FAU - Chromkaew, Yupa AU - Chromkaew Y AUID- ORCID: 0009-0001-7571-6762 AD - Department of Plant and Soil Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand. FAU - Chobyen, Jenjira AU - Chobyen J AD - Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand. FAU - Chinachanta, Kawiporn AU - Chinachanta K AUID- ORCID: 0000-0002-9785-1412 AD - Center of Excellence in Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. AD - Department of Plant and Soil Sciences, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand. FAU - Rangseekaew, Pharada AU - Rangseekaew P AD - Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. AD - Center of Excellence in Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. FAU - Pathom-Aree, Wasu AU - Pathom-Aree W AD - Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. AD - Center of Excellence in Microbial Diversity and Sustainable Utilization, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand. LA - eng GR - FF091/2567/Chiang Mai University/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706179 OTO - NOTNLM OT - bioinoculants OT - plant growth-promoting actinobacteria OT - soil microbiome OT - spent mushroom substrate OT - tomato COIS- The authors declare no conflicts of interest regarding the publication of this article. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:42 PHST- 2026/08/01 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - life16101699 [pii] AID - life-16-01699 [pii] AID - 10.3390/life16101699 [doi] PST - epublish SO - Life (Basel). 2026 Oct 10;16(10):1699. doi: 10.3390/life16101699. PMID- 42942285 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 10 TI - Rapid Assessment of Carotenoid and Gluten Quality Traits in Durum Wheat Hybrids Confirmed by Biochemical and Molecular Marker Analyses. LID - 10.3390/life16101700 [doi] LID - 1700 AB - High levels of carotenoids and gluten are the most important traits for seed quality and colour of semolina produced from durum wheat (Triticum durum Desf.). Rapid methods based on colourimetry with three coordinates L*a*b* and SDS sedimentation produce indirect measurements of carotenoids and gluten, respectively. In contrast, traditional and accurate biochemical methods with n-butanol extraction of carotenoids and a Glutomatic-2200 instrument system for gluten are more complicated approaches requiring specific equipment and much more time. The aim of this study was to test and compare rapid and traditional methods for carotenoid and gluten quality in 8 durum wheat cultivars used as parents and 10 F(3)-F(4) hybrids, which were very diverse in their composition. Genotyping of the TdPsy-B1 and TdGlu-B1 genes using SNP-based ASQ markers was consistent with carotenoid content and gluten quality in the parental lines and hybrids. Strong associations were found in the comparison between rapid measurements and traditional methods for carotenoid and gluten quality. Therefore, colourimetric and SDS sedimentation methods can be used for approximate assessment of carotenoid and gluten quality in durum wheat, but their practical value lies primarily in the ability to rapidly rank and select genotypes for subsequent detailed analysis. FAU - Utebayev, Maral AU - Utebayev M AUID- ORCID: 0000-0003-0729-0592 AD - A.I. Barayev Research and Production Centre of Grain Farming, Shortandy 021601, Kazakhstan. FAU - Dashkevich, Svetlana AU - Dashkevich S AUID- ORCID: 0000-0002-5272-9042 AD - A.I. Barayev Research and Production Centre of Grain Farming, Shortandy 021601, Kazakhstan. FAU - Kradetskaya, Oksana AU - Kradetskaya O AUID- ORCID: 0000-0003-4904-2837 AD - A.I. Barayev Research and Production Centre of Grain Farming, Shortandy 021601, Kazakhstan. FAU - Chilimova, Irina AU - Chilimova I AD - A.I. Barayev Research and Production Centre of Grain Farming, Shortandy 021601, Kazakhstan. FAU - Zhylkybaev, Ruslan AU - Zhylkybaev R AD - A.I. Barayev Research and Production Centre of Grain Farming, Shortandy 021601, Kazakhstan. FAU - Zhigula, Tatyana AU - Zhigula T AD - A.I. Barayev Research and Production Centre of Grain Farming, Shortandy 021601, Kazakhstan. FAU - Shelayeva, Tatyana AU - Shelayeva T AD - A.I. Barayev Research and Production Centre of Grain Farming, Shortandy 021601, Kazakhstan. FAU - Khassanova, Gulmira AU - Khassanova G AD - Institute of Agronomy and Forestry, S. Seifullin Kazakh AgroTechnical Research University, Astana 010000, Kazakhstan. FAU - Kuzbakova, Marzhan AU - Kuzbakova M AUID- ORCID: 0000-0002-1049-4011 AD - Institute of Agronomy and Forestry, S. Seifullin Kazakh AgroTechnical Research University, Astana 010000, Kazakhstan. FAU - Sharipova, Botagoz AU - Sharipova B AUID- ORCID: 0000-0003-2182-8977 AD - Biology Department, Sh. Ualikhanov University, Kokshetau 020000, Kazakhstan. FAU - Shavrukov, Yuri AU - Shavrukov Y AUID- ORCID: 0000-0002-2941-0340 AD - College of Science and Engineering, Biological Sciences, Flinders University, Adelaide, SA 5042, Australia. LA - eng GR - AP 23485115 "KASP markers in the creation and study of tetraploid wheat with increased carot-enoid content and improved gluten quality"/the Science Committee of the Ministry of Science and Higher Education of the Republic of Ka-zakhstan/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705803 OTO - NOTNLM OT - Glutomatic-2200 system OT - SDS sedimentation OT - carotenoids OT - colorimetric method OT - durum wheat OT - genotyping OT - gluten OT - seed quality COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:42 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - life16101700 [pii] AID - life-16-01700 [pii] AID - 10.3390/life16101700 [doi] PST - epublish SO - Life (Basel). 2026 Oct 10;16(10):1700. doi: 10.3390/life16101700. PMID- 42942284 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 10 TI - Proteome-Wide Profiling of Lysine 2-Hydroxyisobutyrylation in Vibrio alginolyticus and Preliminary Association of CobQ with Global Khib Levels. LID - 10.3390/life16101698 [doi] LID - 1698 AB - Vibrio alginolyticus is an important opportunistic pathogen in marine aquaculture, causing vibriosis in a wide range of aquatic animals. Lysine 2-hydroxyisobutyrylation (Khib) is an emerging lysine acylation modification involved in regulating metabolism, stress responses, and pathogenicity in microorganisms. However, the global Khib modification profile and its potential biological functions in V. alginolyticus have remained completely unexplored. This study aims to characterize the Khib modification profile of V. alginolyticus and to preliminarily evaluate the role of CobQ as a candidate regulator of Khib homeostasis. A Khib-modified proteome of V. alginolyticus was constructed using affinity enrichment combined with LC-MS/MS-based modified proteomics. Bioinformatics analyses (GO, COG, KEGG, subcellular localization, motif, and protein-protein interaction) explored the functions of Khib-modified proteins. Selected proteins were analyzed by immunoprecipitation combined with Western blot. A cobQ deletion mutant strain was constructed to assess the association between cobQ deletion and global Khib levels, and to evaluate selected physiological traits. A total of 15,195 Khib sites were identified in 2848 proteins, indicating widespread distribution of Khib modification. Khib-modified proteins were mainly associated with metabolic processes, genetic information processing, signal transduction, membrane biogenesis, and secretion-related pathways. Motif analysis revealed conserved amino acid patterns surrounding Khib sites. Immunoprecipitation combined with Western blot confirmed the Khib modification of TpiA and Ndk at the protein level, but did not validate the specific LC-MS/MS sites. Deletion of cobQ was associated with increased global Khib levels, suggesting that CobQ may be associated with Khib modification in vivo. In vitro assays showed no statistically significant de-Khib activity of CobQ, although a trend toward lower Khib levels was observed. Phenotypic assays showed that cobQ deletion did not significantly affect growth or extracellular protease activity, but reduced swimming motility at low agar concentrations. This study provides a comprehensive catalog of putative Khib-modified proteins of V. alginolyticus. Our findings suggest that CobQ may be associated with global Khib levels, but its direct enzymatic activity and physiological substrates remain to be established. These findings provide a foundational resource for understanding Khib-mediated regulation in V. alginolyticus and identify candidate targets for future mechanistic studies. FAU - Wen, Xiaoxin AU - Wen X AD - Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China. AD - Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, China. FAU - Yang, Shuai AU - Yang S AD - Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China. AD - Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, China. FAU - Liang, Yashu AU - Liang Y AD - Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China. AD - Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, China. FAU - Zhang, Yujia AU - Zhang Y AD - Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China. AD - Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, China. FAU - Wei, Yingzhu AU - Wei Y AD - Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China. AD - Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, China. FAU - Zhang, Weijie AU - Zhang W AD - Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China. AD - Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, China. FAU - Jian, Jichang AU - Jian J AD - Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China. AD - Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, China. FAU - Wang, Na AU - Wang N AUID- ORCID: 0000-0003-0257-5732 AD - Chinese Academy of Inspection and Quarantine, Beijing 100176, China. FAU - Pang, Bijian AU - Pang B AD - Marine Environmental Monitoring Center of Guangxi, Beibu Gulf Marine Ecological Environment Observation and Research Station of Guangxi, Beihai 536000, China. FAU - Pang, Huanying AU - Pang H AD - Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China. AD - Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang 524088, China. LA - eng GR - 2025A1515011061/Natural Science Foundation of Guangdong Province/ GR - No. 202639 and No.202602/Innovation Program in Postgraduate Education of Guangdong Ocean University/ GR - CCTD201802/Undergraduate Innovation Team of Guangdong Ocean University/ GR - 2023GXNSFBA026253/Innovation Program in Postgraduate Education of Guangdong Province, Guangxi Natural Science Foundation/ GR - No. 3267088/National Natural Science Foundation of China/ GR - No. 202408440342/China Scholarship Council/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706536 OTO - NOTNLM OT - CobQ OT - Vibrio alginolyticus OT - lysine 2-hydroxyisobutyrylation modification OT - post-translational modification COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:42 PHST- 2026/08/09 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - life16101698 [pii] AID - life-16-01698 [pii] AID - 10.3390/life16101698 [doi] PST - epublish SO - Life (Basel). 2026 Oct 10;16(10):1698. doi: 10.3390/life16101698. PMID- 42942275 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 9 TI - Can Stochastic Sensing of Amino Acids in Whole Blood, Urine, and Saliva Predict Gastric Tumor Growth and Extension? LID - 10.3390/life16101689 [doi] LID - 1689 AB - Background: Although amino acids have been correlated with gastric cancer progression, most previous investigations have focused on a single biological matrix and have not differentiated between the two enantiomeric forms. Methods: In this study, we investigated the associations between the stereoisomeric profiles of 12 amino acids in whole blood, urine, and saliva and the clinicopathological features of gastric cancer. Samples from 33 patients were analyzed using a stochastic sensing platform, and the data were processed with Excel and MATLAB R1. Results: Distinct amino acid patterns were associated with tumor location, size, thickness, local extension, and pathological T stage. Specific stereoisomeric profiles were associated with tumors extending to the pylorus, duodenum, and esophagus, as well as with disease stage. Conclusions: These findings suggest that a combined analysis of L- and D-amino acid stereoisomers across multiple biological fluids may yield useful minimally invasive molecular profiles for monitoring gastric cancer progression and invasion. FAU - Oraseanu, Alexandra AU - Oraseanu A AUID- ORCID: 0009-0005-4894-3694 AD - Doctoral School of Biomedical Sciences, University of Oradea, 410087 Oradea, Romania. AD - Clinic of Gastroenterology, Bihor Clinical County Emergency Hospital, 410169 Oradea, Romania. FAU - Stefan-van Staden, Raluca-Ioana AU - Stefan-van Staden RI AUID- ORCID: 0000-0001-8244-2483 AD - Laboratory of Electrochemistry and PATLAB, National Institute of Research and Development for Electrochemistry and Condensed Matter, 202 Splaiul Independentei, 060021 Bucharest, Romania. FAU - Georgescu-State, Ramona AU - Georgescu-State R AD - Laboratory of Electrochemistry and PATLAB, National Institute of Research and Development for Electrochemistry and Condensed Matter, 202 Splaiul Independentei, 060021 Bucharest, Romania. FAU - Comnea-Stancu, Ionela Raluca AU - Comnea-Stancu IR AUID- ORCID: 0000-0002-7083-6933 AD - Laboratory of Electrochemistry and PATLAB, National Institute of Research and Development for Electrochemistry and Condensed Matter, 202 Splaiul Independentei, 060021 Bucharest, Romania. FAU - Cioates Negut, Catalina AU - Cioates Negut C AUID- ORCID: 0000-0001-7307-0075 AD - Laboratory of Electrochemistry and PATLAB, National Institute of Research and Development for Electrochemistry and Condensed Matter, 202 Splaiul Independentei, 060021 Bucharest, Romania. FAU - Tuchiu-Stanca, Bianca-Maria AU - Tuchiu-Stanca BM AUID- ORCID: 0000-0003-0379-0880 AD - Laboratory of Electrochemistry and PATLAB, National Institute of Research and Development for Electrochemistry and Condensed Matter, 202 Splaiul Independentei, 060021 Bucharest, Romania. FAU - Gheorghe, Damaris-Cristina AU - Gheorghe DC AUID- ORCID: 0000-0002-6430-2177 AD - Laboratory of Electrochemistry and PATLAB, National Institute of Research and Development for Electrochemistry and Condensed Matter, 202 Splaiul Independentei, 060021 Bucharest, Romania. FAU - Ilie-Mihai, Ruxandra-Maria AU - Ilie-Mihai RM AUID- ORCID: 0000-0002-2285-9583 AD - Laboratory of Electrochemistry and PATLAB, National Institute of Research and Development for Electrochemistry and Condensed Matter, 202 Splaiul Independentei, 060021 Bucharest, Romania. FAU - Stefan, Andrada-Ioana AU - Stefan AI AUID- ORCID: 0009-0005-9437-4007 AD - Laboratory of Electrochemistry and PATLAB, National Institute of Research and Development for Electrochemistry and Condensed Matter, 202 Splaiul Independentei, 060021 Bucharest, Romania. FAU - van Staden, Jacobus Koos Frederick AU - van Staden JKF AUID- ORCID: 0000-0002-9684-005X AD - Laboratory of Electrochemistry and PATLAB, National Institute of Research and Development for Electrochemistry and Condensed Matter, 202 Splaiul Independentei, 060021 Bucharest, Romania. FAU - Brisc, Ciprian AU - Brisc C AUID- ORCID: 0000-0003-4416-2848 AD - Doctoral School of Biomedical Sciences, University of Oradea, 410087 Oradea, Romania. AD - Clinic of Gastroenterology, Bihor Clinical County Emergency Hospital, 410169 Oradea, Romania. FAU - Bratei, Alexandru Adrian AU - Bratei AA AUID- ORCID: 0000-0003-3758-2289 AD - Laboratory of Electrochemistry and PATLAB, National Institute of Research and Development for Electrochemistry and Condensed Matter, 202 Splaiul Independentei, 060021 Bucharest, Romania. AD - Clinical Nephrology Hospital "Carol Davila", 4 Grivitei Street, 010731 Bucharest, Romania. LA - eng GR - PN 23 27 03 01/Ministerul Cercetarii si Inovarii/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705984 OTO - NOTNLM OT - amino acids OT - enantiomers OT - gastric cancer COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:42 PHST- 2026/08/16 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - life16101689 [pii] AID - life-16-01689 [pii] AID - 10.3390/life16101689 [doi] PST - epublish SO - Life (Basel). 2026 Oct 9;16(10):1689. doi: 10.3390/life16101689. PMID- 42942271 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 9 TI - Integrated Transcriptomic and Metabolomic Analyses Reveal the Regulatory Networks of Cuticular Wax Fatty Acid Metabolism in Jujube (Ziziphus jujuba Mill. 'Hupingzao'). LID - 10.3390/life16101685 [doi] LID - 1685 AB - The cuticular wax of 'Hupingzao,' an important economic crop in China, not only constitutes a key factor in determining the appearance quality and gloss of the fruit but also plays a vital role in regulating water evaporation and resisting biotic and abiotic stresses. In this study, 'Hupingzao' fruit produced during the white-ripe (WR) and full-red (FR) periods were used as study materials. The key genes and components of wax development were screened using a combination of transcriptomics and metabolomics. Enrichment analysis was performed on the differential genes and metabolites, and the fatty acid biosynthesis and elongation pathways were selected for further study. A series of key candidate genes in the de novo synthesis, elongation, and subsequent modification pathways of fatty acids were identified, such as acetyl-CoA carboxylase (ACCase), fabF (beta-Ketoacyl-ACP Synthase II), and beta-ketoacyl-CoA synthase (KCS). To further explore the mechanism of jujube wax biosynthesis, GC-MS (gas chromatography-mass spectrometry) was used to determine and analyze the content and composition of the wax after it was treated with gibberellin and paclobutrazol. The results showed that spraying the plants with GA(3) significantly upregulated the expression of genes involved in fatty acid de novo synthesis and elongation such as accC (Acetyl-CoA Carboxylase, Biotin Carboxylase Subunit), fabD (Malonyl-CoA:ACP Transacylase), fabG (beta-Ketoacyl-ACP Reductase), fabI (Enoyl-ACP Reductase), ACSL (Long-chain Acyl-CoA Synthetase), and KCS and increased the content of dodecanoic acid, behenic acid, lignoceric acid, and hexacosanoic acid. These results suggest that the GA signal may participate in the upregulation of fatty acid synthesis. In this study, we systematically constructed a molecular map of wax fatty acid metabolism during the fruit development stage of the 'Hupingzao' variety, providing an important theoretical basis and candidate genes for future directional regulation of wax components via molecular breeding or cultivation measures. FAU - Li, Na AU - Li N AD - Department of Biological Science and Technology, Jinzhong University, Jinzhong 030609, China. FAU - Song, Yuqin AU - Song Y AUID- ORCID: 0009-0001-5904-6957 AD - College of Horticulture, Shanxi Agricultural University, Taigu 030801, China. FAU - Feng, Xinxin AU - Feng X AD - College of Horticulture, Shanxi Agricultural University, Taigu 030801, China. FAU - Ji, Aiqing AU - Ji A AD - Department of Biological Science and Technology, Jinzhong University, Jinzhong 030609, China. FAU - Han, Hongyan AU - Han H AD - Department of Biological Science and Technology, Jinzhong University, Jinzhong 030609, China. FAU - Ji, Pengyu AU - Ji P AD - Department of Biological Science and Technology, Jinzhong University, Jinzhong 030609, China. FAU - Li, Liulin AU - Li L AD - College of Horticulture, Shanxi Agricultural University, Taigu 030801, China. LA - eng GR - 202303021222261/Shanxi Provincial Department of Science and Technology/ GR - JUD2022002/Jinzhong University/ GR - J20241325/Shanxi Provincial Department of Education/ GR - KY00655/Shanxi Zhongzhizhi Agriculture and Forestry Technology Co., Ltd./ GR - S202502/the Jinzhong City Self-initiated scientific research projects/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706060 OTO - NOTNLM OT - fruit development OT - gibberellin OT - very-long-chain fatty acids OT - wax metabolites OT - beta-Ketoacyl-CoA Synthase (KCS) COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:42 PHST- 2026/08/18 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - life16101685 [pii] AID - life-16-01685 [pii] AID - 10.3390/life16101685 [doi] PST - epublish SO - Life (Basel). 2026 Oct 9;16(10):1685. doi: 10.3390/life16101685. PMID- 42942266 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 8 TI - Spathology: The Birth of Spatial Pathology as a Discipline. LID - 10.3390/life16101680 [doi] LID - 1680 AB - Pathology has always been a spatial science: for more than 150 years, diagnosis has rested on reading the architecture of cells and tissues. Three technological streams are now converging on this founding principle. Spatially resolved omics can measure thousands of transcripts and over a hundred proteins in intact, formalin-fixed paraffin-embedded (FFPE) tissue; multiplexed imaging quantifies tissue microenvironments cell by cell; and computational pathology has progressed from slide digitization to foundation models that can predict molecular features from morphology. The clinical evidence for spatial readouts is real but heterogeneous: a few assays have reached guideline mention or prospective use, while most findings remain retrospective, platform-dependent, and unevenly validated. Yet the field remains fragmented across "spatial biology", "digital pathology", and "computational pathology", without a unified framework for standards, training, and diagnostics. Here we propose, for discussion rather than as a settled matter, that this convergence be treated as a coherent branch of pathology, "spatial pathology", which we define as the use of spatially resolved measurements of tissue to derive diagnostic, prognostic, and predictive information. We set out which problems existing structures do not yet address, acknowledge the counterarguments, and outline a consensus-building agenda. We also suggest "spathology" as an optional shorthand, and we disclose openly that all authors are co-founders of a company, SPATHO AB, whose name derives from the same contraction. FAU - Long, Mengping AU - Long M AD - Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, 171 65 Solna, Sweden. FAU - Nilsson, Mats AU - Nilsson M AD - Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, 171 65 Solna, Sweden. FAU - Hu, Taobo AU - Hu T AUID- ORCID: 0000-0001-5124-7167 AD - Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, 171 65 Solna, Sweden. LA - eng PT - Comment PT - Journal Article DEP - 20261008 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706112 OTO - NOTNLM OT - artificial intelligence OT - biomarker discovery OT - computational pathology OT - digital pathology OT - multiplexed imaging OT - spathology OT - spatial pathology OT - spatial proteomics OT - spatial transcriptomics OT - tumor microenvironment COIS- We wish to be fully transparent. All three authors are co-founders of SPATHO AB and hold financial interests in it; SPATHO AB develops technologies and analytical methods in spatial pathology, and its name derives from the same contraction as the shorthand proposed here. Advocacy for a term associated with the authors' company is therefore a real conflict of interest, and readers should weigh the proposal accordingly; we have tried to present the counterarguments and to make the framework independent of the shorthand. The article is a purely academic discussion: it does not describe, evaluate, or promote any product, service, or technology of SPATHO AB, presents no company data, and its arguments do not depend on the company's existence or activities; the work received no external funding. SPATHO AB has made no commitment regarding the use of these terms, and readers should be aware that the company may seek to protect them in the future. In addition, T.H. is a Guest Editor of the Special Issue "Advances in Computational and Spatial Pathology" in which this article appears. T.H. had no role in the editorial handling of this manuscript, the selection of reviewers, or the decision on it. We also acknowledge that publication of this article may benefit the Special Issue, and readers should weigh this as well. Mengping Long, Mats Nilsson, and Taobo Hu are co-founders of SPATHO AB and have financial interests in the company. SPATHO AB is a Swedish company developing technologies and analytical methods in spatial pathology, and its name derives from the same contraction as the term "spathology" discussed in this article. The authors' advocacy of the term is therefore a potential conflict of interest, discussed in Section 5.3. The article is a purely academic discussion and does not describe, evaluate, or promote any product or service of the company. T.H. serves as Guest Editor of the Special Issue "Advances in Computational and Spatial Pathology" and had no role in the editorial handling of this manuscript, the selection of reviewers, or the decision on it. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:42 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - life16101680 [pii] AID - life-16-01680 [pii] AID - 10.3390/life16101680 [doi] PST - epublish SO - Life (Basel). 2026 Oct 8;16(10):1680. doi: 10.3390/life16101680. PMID- 42942263 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 7 TI - Maternal Characteristics, First-Trimester Biomarkers and Cell-Free DNA Fetal Fraction: A Single-Center NIPT Cohort over Ten Years. LID - 10.3390/life16101677 [doi] LID - 1677 AB - The fetal fraction (FF) of cell-free DNA determines the reliability of non-invasive prenatal testing (NIPT); whether first-trimester placental biomarkers add information beyond maternal factors is unclear. We linked NIPT samples from a single fetal medicine center over ten years (1162 pregnancies) to first-trimester screening records and modeled log FF with hierarchical regression (maternal/technical covariates --> PAPP-A --> free beta-hCG), cluster-robust standard errors, and an order-independent bootstrapped partition of the biomarker contribution. In the primary complete-case model (n = 292), FF was 3.4% lower per kg/m(2) of BMI (95% CI -4.4 to -2.3) and 24% lower after IVF/ICSI. Free beta-hCG was independently associated with FF (+11.0% per doubling of MoM, 95% CI +5.3 to +16.9; semi-partial R(2) = 0.046), whereas the PAPP-A association was weaker and less consistent (+4.8%, 95% CI -0.6 to +10.4; semi-partial R(2) = 0.010). The beta-hCG association persisted after excluding high-risk NIPT calls, documented multiple gestation and IVF/ICSI, within platform-restricted subsets and with a restricted documentation-to-draw interval. No independent association with gestational age was identified in the primary model; low-FF and no-call results were too few for adjusted inference. Free beta-hCG carries independent information on FF, consistent with, but not proof of, a link to syncytiotrophoblast biology. FAU - Muresan, Maria Cezara AU - Muresan MC AUID- ORCID: 0000-0003-2661-8485 AD - Department of Obstetrics and Gynecology, Faculty of Medicine, Victor Babes University of Medicine and Pharmacy, 300041 Timisoara, Romania. FAU - Muresan, Marius Bogdan AU - Muresan MB AUID- ORCID: 0009-0000-9378-3435 AD - Maternal-Fetal Medicine Center, 300331 Timisoara, Romania. FAU - Biris, Dan Dumitrascu AU - Biris DD AUID- ORCID: 0000-0002-4108-0452 AD - Maternal-Fetal Medicine Center, 300331 Timisoara, Romania. FAU - Popescu, Daniela-Eugenia AU - Popescu DE AUID- ORCID: 0000-0002-4986-8025 AD - Department of Obstetrics and Gynecology, Faculty of Medicine, Victor Babes University of Medicine and Pharmacy, 300041 Timisoara, Romania. LA - eng PT - Journal Article DEP - 20261007 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705701 OTO - NOTNLM OT - PAPP-A OT - body mass index OT - cell-free DNA OT - fetal fraction OT - first-trimester screening OT - free beta-hCG OT - in vitro fertilization OT - non-invasive prenatal testing OT - test failure COIS- The authors declare no conflicts of interest. M.B.M. is a representative of the institution that holds the clinical database; this dual role is declared transparently in the ethics approval. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/07 CRDT- 2026/10/27 10:42 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - life16101677 [pii] AID - life-16-01677 [pii] AID - 10.3390/life16101677 [doi] PST - epublish SO - Life (Basel). 2026 Oct 7;16(10):1677. doi: 10.3390/life16101677. PMID- 42942262 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 7 TI - Next-Generation Pharmacotherapy for Uterine Leiomyomas: Combining Metformin and Ulipristal Acetate as Neoadjuvant and Adjuvant Interventions to Minimally Invasive Procedures. LID - 10.3390/life16101676 [doi] LID - 1676 AB - Uterine fibroids (leiomyomas) are the most common benign tumors of the myometrium in women of reproductive age. They are a major cause of heavy menstrual bleeding, pelvic pressure and pain, and reduced fertility in women of reproductive age. This scoping review aims to examine the potential of two existing medications, metformin and ulipristal acetate (UPA), to treat fibroids individually and in combination with current minimally invasive procedures. Studies published between January 2016 and May 2026 were identified from four databases: PubMed, Scopus, EBSCO, and Google Scholar. Of the 2583 papers identified, 23 studies met the inclusion criteria for this review. Ulipristal acetate (UPA) has been shown to decrease heavy menstrual bleeding and uterine fibroid volume in several clinical studies. However, its use as a treatment for uterine fibroids has been severely restricted in several countries because of the rare occurrence of idiosyncratic drug-induced liver injury. Metformin acts on multiple pathways reported to be involved in the pathogenesis of leiomyomas, including activation of the energy sensor AMP-activated protein kinase (AMPK), inhibition of the mammalian target of rapamycin (mTOR) signaling pathway, modulation of steroidogenesis, suppression of angiogenesis, and induction of apoptosis. The complementary molecular mechanisms of action of metformin and UPA provide a theoretical basis for investigating combination therapy. However, there is currently a lack of direct evidence regarding the potential for synergistic reduction in fibroid volume or regression with combination treatment, and no clinical trials have investigated the use of metformin in conjunction with UPA for the treatment of uterine fibroids in humans. Future studies of metformin and UPA may also benefit from the development of targeted intrauterine and intralesional delivery systems to increase local drug exposure. However, the pharmacokinetics, tissue penetration, hepatic safety, and clinical efficacy of such systems remain to be established. Therefore, while the available evidence supports further investigation of metformin and UPA individually as potential therapeutic agents for uterine fibroids, their combined use, as well as their use in conjunction with current minimally invasive procedures for fibroid treatment, should be regarded as investigational and requires further preclinical and prospective clinical evaluation. FAU - Rozmurat, Nuray AU - Rozmurat N AD - School of Medicine, Nazarbayev University, Zhanybek-Kerey Khans Street 5/1, Astana 010000, Kazakhstan. FAU - Aimagambetova, Gulzhanat AU - Aimagambetova G AUID- ORCID: 0000-0002-2868-4497 AD - Department of Surgery, School of Medicine, Nazarbayev University, Zhanybek-Kerey Khans Street 5/1, Astana 010000, Kazakhstan. AD - Clinical Academic Department of Women's Health, CF "University Medical Center", Turan Ave. 32, Astana 010000, Kazakhstan. FAU - Terzic, Sanja AU - Terzic S AUID- ORCID: 0000-0002-0034-2559 AD - Department of Medicine, School of Medicine, Nazarbayev University, Zhanybek-Kerey Khans Street 5/1, Astana 010000, Kazakhstan. FAU - Zhao, Peng AU - Zhao P AD - Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China. AD - Zhejiang Provincial Clinical Research Center for Reproductive Health and Disease, Hangzhou 310016, China. AD - Zhejiang Key Laboratory of Precise Protection and Promotion of Fertility, Hangzhou 310016, China. FAU - Bapayeva, Gauri AU - Bapayeva G AUID- ORCID: 0000-0002-8344-7873 AD - Clinical Academic Department of Women's Health, CF "University Medical Center", Turan Ave. 32, Astana 010000, Kazakhstan. FAU - Kongrtay, Kuralay AU - Kongrtay K AUID- ORCID: 0000-0002-1405-2245 AD - Department of Surgery, School of Medicine, Nazarbayev University, Zhanybek-Kerey Khans Street 5/1, Astana 010000, Kazakhstan. AD - Clinical Academic Department of Women's Health, CF "University Medical Center", Turan Ave. 32, Astana 010000, Kazakhstan. FAU - Tanko, Matthew Naanlep AU - Tanko MN AUID- ORCID: 0000-0003-1270-1886 AD - Department of Medicine, School of Medicine, Nazarbayev University, Zhanybek-Kerey Khans Street 5/1, Astana 010000, Kazakhstan. FAU - Abdrakhmanova, Ainur AU - Abdrakhmanova A AD - School of Medicine, Nazarbayev University, Zhanybek-Kerey Khans Street 5/1, Astana 010000, Kazakhstan. FAU - Lagana, Antonio Simone AU - Lagana AS AUID- ORCID: 0000-0003-1543-2802 AD - Obstetrics and Gynecology Unit, Ospedali Riuniti Villa Sofia-Cervello, Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties (PROMISE), University of Palermo, 90121 Palermo, Italy. FAU - Terzic, Milan AU - Terzic M AUID- ORCID: 0000-0003-3914-5154 AD - Department of Surgery, School of Medicine, Nazarbayev University, Zhanybek-Kerey Khans Street 5/1, Astana 010000, Kazakhstan. AD - Clinical Academic Department of Women's Health, CF "University Medical Center", Turan Ave. 32, Astana 010000, Kazakhstan. LA - eng GR - AP26194421/Ministry of Science and Higher Education of the Republic of Kazakhstan/ PT - Journal Article PT - Review DEP - 20261007 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706164 OTO - NOTNLM OT - metformin OT - ulipristal acetate OT - uterine fibroids OT - uterine leiomyoma COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/07 CRDT- 2026/10/27 10:42 PHST- 2026/08/25 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - life16101676 [pii] AID - life-16-01676 [pii] AID - 10.3390/life16101676 [doi] PST - epublish SO - Life (Basel). 2026 Oct 7;16(10):1676. doi: 10.3390/life16101676. PMID- 42942259 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 7 TI - A Comparative Analysis of the Ecological Niches of Erigeron canadensis L. and Erigeron annuus (L.) Pers. in Invasive Areas and Their Native Habitats. LID - 10.3390/life16101674 [doi] LID - 1674 AB - Biological invasions are a major threat to biodiversity; analyzing changes in the ecological niches of invasive species is of great significance for the prevention and control of biological invasions. Heilongjiang Province, which serves as a crucial ecological security barrier for China, has also faced invasions by invasive Asteraceae plants in recent years; however, the invasion patterns and ecological adaptation characteristics of these invasive plants remain unclear. Therefore, this study selected the representative Asteraceae species, E. canadensis and E. annuus, as the subjects of investigation. Based on data from the Heilongjiang invasive plant survey and supplementary field survey data, and in combination with distribution data from their native range in North America, the study employed methods such as ecological niche analysis and ecological niche overlap comparison and used Schoener's D index. This study evaluates the potential suitable habitats for E. canadensis and E. annuus in Heilongjiang Province and analyzes the characteristics of niche shifts between their native and invaded habitats. The results indicate that both invasive plant species exhibit strong ecological conservatism; however, their ecological adaptation strategies in Heilongjiang Province differ, with E. annuus showing a higher degree of spread compared to E. canadensis. FAU - Liu, Wansheng AU - Liu W AD - College of Forestry, Northeast Forestry University, Harbin 150040, China. FAU - Liu, Jiale AU - Liu J AD - College of Forestry, Northeast Forestry University, Harbin 150040, China. FAU - Zhou, Mo AU - Zhou M AD - Horticultural Branch, Heilongjiang Academy of Agricultural Sciences, Harbin 150069, China. FAU - Mu, Liqiang AU - Mu L AUID- ORCID: 0000-0002-2962-703X AD - College of Forestry, Northeast Forestry University, Harbin 150040, China. LA - eng GR - ZQTYB240100013/Survey of National Key Protected Wild Plants in Heilongjiang Province/ PT - Journal Article DEP - 20261007 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706428 OTO - NOTNLM OT - Erigeron annuus (L.) Pers. OT - Erigeron canadensis L. OT - biological invasions OT - ecological niche OT - spatial niche overlap COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/07 CRDT- 2026/10/27 10:42 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - life16101674 [pii] AID - life-16-01674 [pii] AID - 10.3390/life16101674 [doi] PST - epublish SO - Life (Basel). 2026 Oct 7;16(10):1674. doi: 10.3390/life16101674. PMID- 42942258 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 7 TI - The TET Protein Family in Gliomas: Epigenetic Regulation, Molecular Pathogenesis, and Therapeutic Opportunities. LID - 10.3390/life16101672 [doi] LID - 1672 AB - Epigenetic dysregulation is a defining feature of glioma pathogenesis, with aberrant DNA methylation contributing to tumor initiation, cellular plasticity, progression, and therapeutic resistance. The ten-eleven translocation (TET) family of dioxygenases (TET1, TET2, and TET3) catalyzes the 5-methylcytosine (5mC) oxidation to 5-hydroxymethylcytosine (5hmC) and subsequent intermediates, inducingactive DNA demethylation and regulating organization of chromatin and gene expression. In gliomas, particularly those harboring isocitrate dehydrogenase (IDH) mutations, accumulation of the 2-hydroxyglutarate oncometabolite suppresses TET activity, leading to widespread epigenetic remodeling, the glioma CpG island methylator phenotype and impaired cellular differentiation. Emerging evidence demonstrates that individual TET isoforms exert distinct and context-dependent biological functions. Although TET1, TET2, and TET3 have been involved in the regulation of glioma biology, their effects may vary depending on the cellular and molecular context, with individual isoforms exhibiting distinct and, in some cases, opposing effects on glioma behavior. This review summarizes current knowledge regarding the molecular mechanisms governing TET dysregulation in gliomas, including their interactions with metabolic alterations, chromatin remodeling, the tumor microenvironment, and immune evasion. Recent advances in methodologies for assessing TET activity and 5hmC levels are also discussed along with the possible integration of TET-associated biomarkers into molecular glioma classification, and emerging therapeutic strategies to restore or selectively modulate TET function. FAU - Kandilas, Angelos AU - Kandilas A AUID- ORCID: 0009-0004-9187-8421 AD - Department of Biological Chemistry, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Leimonis, Dionisis AU - Leimonis D AUID- ORCID: 0009-0002-6405-983X AD - Department of Biological Chemistry, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Vasilakis, Ioannis AU - Vasilakis I AD - Department of Biological Chemistry, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Kosma, Elisavet AU - Kosma E AD - Department of Biological Chemistry, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Piperi, Christina AU - Piperi C AUID- ORCID: 0000-0002-2701-0618 AD - Department of Biological Chemistry, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece. LA - eng PT - Journal Article PT - Review DEP - 20261007 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705798 OTO - NOTNLM OT - 5hmC OT - DNA methylation OT - IDH mutation OT - TET proteins OT - brain tumors OT - epigenetic therapy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/07 CRDT- 2026/10/27 10:42 PHST- 2026/08/01 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - life16101672 [pii] AID - life-16-01672 [pii] AID - 10.3390/life16101672 [doi] PST - epublish SO - Life (Basel). 2026 Oct 7;16(10):1672. doi: 10.3390/life16101672. PMID- 42942256 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 7 TI - From Antimicrobial Biomaterials to Infection-Resilient Interfaces: Engineering the Host, Microbe, and Material Interface Against Drug-Resistant Biofilms. LID - 10.3390/life16101670 [doi] LID - 1670 AB - Drug-resistant biofilm infections remain a major challenge for medical devices and implants. Biofilm formation can reduce antimicrobial effectiveness through physiological heterogeneity, tolerance, persistence, and interactions with the host and biomaterial surface. Antimicrobial biomaterials have been developed to prevent colonization, detect infection, provide antimicrobial activity, control established biofilms, and support tissue recovery. However, their performance depends on the biological context in which they function. This review examines these strategies through interactions among the host, microbe, and material, using carbapenem-resistant Acinetobacter baumannii, methicillin-resistant Staphylococcus aureus, and carbapenem-resistant Pseudomonas aeruginosa as selected biological stress-test examples for evaluating biomaterial performance. It also considers the limitations in current evaluation, including planktonic testing, biological conditioning, microbial adaptation, effects on non-target microorganisms, material stability, manufacturing, and clinical translation. Antimicrobial activity alone does not establish effective performance in infection. Material function must match the microbial state, host environment, and intended clinical use while maintaining host compatibility and function during use. We propose infection resilience as a design framework organized around Prevent, Detect, Respond, Resolve, and Regenerate. Infection resilience refers to coordinated material performance under changing microbial and host conditions rather than the number of functions incorporated into a material. This framework offers a practical basis for more biologically relevant evaluation and purposeful design of antimicrobial biomaterials for drug-resistant biofilm infections. FAU - Elbehiry, Ayman AU - Elbehiry A AUID- ORCID: 0000-0001-7726-9532 AD - Department of Public Health, College of Applied Medical Sciences, Qassim University, P.O. Box 6666, Buraydah 51452, Saudi Arabia. FAU - Abalkhail, Adil AU - Abalkhail A AD - Department of Public Health, College of Applied Medical Sciences, Qassim University, P.O. Box 6666, Buraydah 51452, Saudi Arabia. FAU - Marzouk, Eman AU - Marzouk E AUID- ORCID: 0000-0003-1699-6263 AD - Department of Public Health, College of Applied Medical Sciences, Qassim University, P.O. Box 6666, Buraydah 51452, Saudi Arabia. FAU - Bayameen, Abdulraheem AU - Bayameen A AD - Family Medicine Department, King Fahad Armed Forces Hospital, Jeddah 23311, Saudi Arabia. FAU - Ashour, Shaimaa AU - Ashour S AD - Family Medicine Department, King Fahad Armed Forces Hospital, Jeddah 23311, Saudi Arabia. FAU - Yousuf, Faisal AU - Yousuf F AD - Family Medicine Department, King Fahad Armed Forces Hospital, Jeddah 23311, Saudi Arabia. FAU - Albugami, Fahad AU - Albugami F AD - Human Resources Department, Prince Mansour Military Hospital, Taif 26526, Saudi Arabia. FAU - Anagreyyah, Sulaiman AU - Anagreyyah S AD - Family Medicine Department, King Fahad Armed Forces Hospital, Jeddah 23311, Saudi Arabia. FAU - Almuzaini, Abdulaziz M AU - Almuzaini AM AUID- ORCID: 0000-0001-9997-7476 AD - Department of Veterinary Preventive Medicine, College of Veterinary Medicine, Qassim University, Buraydah 51452, Saudi Arabia. FAU - Ibrahem, Mai AU - Ibrahem M AD - Department of Public Health, College of Applied Medical Science, King Khalid University, Abha 61421, Saudi Arabia. FAU - Edrees, Husam M AU - Edrees HM AD - Department of Physiology, Faculty of Medicine, University of Tabuk, Tabuk 71491, Saudi Arabia. LA - eng PT - Journal Article PT - Review DEP - 20261007 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706135 OTO - NOTNLM OT - antibiotic resistance OT - antimicrobial biomaterials OT - antimicrobial surfaces OT - biofilm control OT - biomaterial-associated infection OT - drug-resistant biofilms OT - host-microbe-material interactions OT - infection-resilient interfaces OT - medical devices COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/07 CRDT- 2026/10/27 10:42 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - life16101670 [pii] AID - life-16-01670 [pii] AID - 10.3390/life16101670 [doi] PST - epublish SO - Life (Basel). 2026 Oct 7;16(10):1670. doi: 10.3390/life16101670. PMID- 42942255 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 7 TI - The Obstructive Sleep Apnea Care Ecosystem in the United States: Clinical Pathways and the Evolving Role of Otolaryngology. LID - 10.3390/life16101669 [doi] LID - 1669 AB - Obstructive sleep apnea (OSA) is a common, heterogeneous disorder managed through a multidisciplinary pathway that connects recognition, physiologic testing, treatment selection, specialty referral, insurance coverage, and longitudinal reassessment. This narrative review provides a clinically oriented, system-level overview of the adult OSA care ecosystem in the United States for an international clinical audience, with particular emphasis on the evolving role of otolaryngology. We describe the organization and complementary functions of sleep physicians, otolaryngologists, dentists, and allied health professionals. We also outline the roles of polysomnography and home sleep apnea testing, along with major therapeutic pathways including positive airway pressure, oral appliance therapy, weight management, upper-airway surgery, and neurostimulation. Otolaryngology provides an important interface between physiologic diagnosis and airway-specific treatment by integrating anatomic evaluation, sleep-state airway phenotyping, intervention selection, and post-treatment reassessment. Recent advances in neurostimulation, pharmacologic weight management, wearable and home-based monitoring, airflow analysis, and artificial intelligence are expanding opportunities for accessible and phenotype-informed care. Important challenges include underdiagnosis, treatment attrition, fragmented transitions, variable coverage, and reliance on the apnea-hypopnea index. Although health-care structures differ internationally, multidisciplinary coordination, individualized treatment selection, and longitudinal reassessment provide broadly applicable principles for developing patient-centered OSA programs. FAU - Tu, Xinhang AU - Tu X AD - Sleep Disorders Center, Department of Neurology, University of Michigan, Ann Arbor, MI 48109, USA. FAU - Schwartz, Alan R AU - Schwartz AR AUID- ORCID: 0000-0001-9222-3751 AD - Department of Otolaryngology-Head and Neck Surgery, Vanderbilt University Medical Center, 1215 21st Ave. S, Medical Center East, Nashville, TN 37232, USA. AD - Department of Otorhinolaryngology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA. AD - School of Medicine, Universidad Peruana Cayetano Heredia, Lima 15102, Peru. FAU - Wang, Yichen AU - Wang Y AD - Division of Gastroenterology and Hepatology, Mayo Clinic, Jacksonville, FL 32224, USA. FAU - Huang, Yuting AU - Huang Y AUID- ORCID: 0000-0001-9986-5124 AD - Division of Gastroenterology and Hepatology, Mayo Clinic, Jacksonville, FL 32224, USA. FAU - Li, Yike AU - Li Y AUID- ORCID: 0000-0001-8465-130X AD - Department of Otolaryngology-Head and Neck Surgery, Vanderbilt University Medical Center, 1215 21st Ave. S, Medical Center East, Nashville, TN 37232, USA. LA - eng PT - Journal Article PT - Review DEP - 20261007 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706391 OTO - NOTNLM OT - drug-induced sleep endoscopy OT - neurostimulation OT - obstructive sleep apnea OT - otolaryngology OT - polysomnography OT - positive airway pressure OT - upper-airway phenotyping OT - weight management COIS- Schwartz serves as a scientific advisor for Apnimed, HuMannity Foundation, Itamar/Zoll, LivaNova, Lunair, Nyxoah SA, Periodic Breathing LLC, Respicardia/Zoll, Restera, Inc. and Takeda. The other authors declare no competing interests. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/07 CRDT- 2026/10/27 10:42 PHST- 2026/08/01 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - life16101669 [pii] AID - life-16-01669 [pii] AID - 10.3390/life16101669 [doi] PST - epublish SO - Life (Basel). 2026 Oct 7;16(10):1669. doi: 10.3390/life16101669. PMID- 42942254 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 6 TI - Selective YAP1 Transcriptional Response to Graded Retinoic Acid Exposure in NTERA-2 Cells. LID - 10.3390/life16101668 [doi] LID - 1668 AB - Background: Retinoic acid (RA) regulates neural cell-state transitions, but its effects on Hippo-pathway effectors in NTERA-2 cells remain incompletely defined. Methods: NTERA-2 cultures were exposed for seven days to 5, 10, or 15 microM RA, with untreated and 0.1% DMSO controls (n = 3 parallel flasks per group from the same passage and starting suspension). YAP1, WWTR1, NPTN, and miR-148a-3p expressions were quantified by RT-qPCR. RA-versus-DMSO comparisons were evaluated on the -DeltaCq scale with false-discovery-rate correction and sensitivity analyses. Results: Metabolic viability remained at 95-97% of untreated-control values. Relative to DMSO, YAP1 expression was 9.73-fold and 8.14-fold higher at 5 and 10 microM RA, respectively (q = 0.0462 for both), although levels remained below untreated control and direct RA-versus-untreated comparisons were nonsignificant. No corrected differences were detected for WWTR1 or NPTN. miR-148a-3p comparisons were nonsignificant and interpreted cautiously because miR-26b-5p showed condition-dependent variation. Conclusions: Seven-day RA exposure was associated with a selective YAP1 transcriptional response relative to the DMSO background at 5-10 microM. The pattern is also compatible with partial attenuation of a vehicle-associated reduction rather than induction above baseline. These transcript-level data do not establish neuronal maturation or Hippo-pathway activation. FAU - Ay, Mustafa Ertan AU - Ay ME AUID- ORCID: 0009-0002-5330-8554 AD - Department of Medical Biology and Genetics, Faculty of Medicine, Mersin University, 33343 Mersin, Turkiye. FAU - Cevik, Kenan AU - Cevik K AD - Department of Medical Biology and Genetics, Faculty of Medicine, Mersin University, 33343 Mersin, Turkiye. FAU - Izci Ay, Ozlem AU - Izci Ay O AUID- ORCID: 0000-0002-4847-6943 AD - Department of Medical Biology and Genetics, Faculty of Medicine, Mersin University, 33343 Mersin, Turkiye. LA - eng GR - 2023-1-AP3-4898/Mersin Universitesi/ PT - Journal Article DEP - 20261006 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705999 OTO - NOTNLM OT - Hippo pathway OT - NTERA-2 OT - RT-qPCR OT - YAP1/WWTR1/NPTN OT - miR-148a-3p OT - retinoic acid COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/06 CRDT- 2026/10/27 10:42 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/06 00:00 [pmc-release] AID - life16101668 [pii] AID - life-16-01668 [pii] AID - 10.3390/life16101668 [doi] PST - epublish SO - Life (Basel). 2026 Oct 6;16(10):1668. doi: 10.3390/life16101668. PMID- 42942249 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 3 TI - Marker-Assisted Selection of Soybean E-Locus Alleles Using Functional and Linked Markers: Phenotypic Validation Under High-Latitude Conditions. LID - 10.3390/life16101663 [doi] LID - 1663 AB - Photoperiod sensitivity is one of the main factors limiting soybean adaptation to high-latitude conditions. The aim of this study was to perform marker-assisted selection (MAS) at the E1, E3, E4, and E7 loci in three soybean F(2) populations (F10, F13, and U4), followed by field evaluation of their F(3) progenies in Northern Kazakhstan. Individual plants were genotyped with functional PCR-based markers for E1, E3, and E4, and with linked SSR markers for E7. This allowed us to select plants carrying target alleles and marker profiles. In total, 110 F(3) progenies were tested in the Kostanay Region, and 80 of them (72.7%) reached full maturity. The phenotypic associations of the E loci and differed among genetic backgrounds. In F10, e3-fs was strongly associated with earlier flowering and completion of maturation. In F13, e4-SORE-I was associated with earlier flowering, a higher frequency of completed maturation, and earlier maturity. Also in F13, SSR marker classes associated with E7 were significantly related to maturity status and days to maturity. Among the multilocus classes in F13, the target combination e3-fs/e3-fs e4-SORE-I/e4-SORE-I together with the SSR marker profile associated with e7/e7, showed the earliest mean flowering and maturation times. Based on the field results, 14 F(3) progenies were selected for further breeding work. These progenies completed maturation within 90-110 days and met additional agronomic selection criteria. Hybridization combined with MAS enabled targeted selection of breeding material carrying the valuable alleles e1-nl, e3-fs, and e4-SORE-I, as well as favorable multilocus profiles. The results highlight the value of combining multilocus MAS with field evaluation in the target environment for developing early-maturing, photoperiod-adapted soybean breeding material for Northern Kazakhstan. FAU - Maikotov, Bekzhan AU - Maikotov B AD - Plant Biotechnology Laboratory, Kazakh Research Institute of Agriculture and Plant Growing, Almalybak 040909, Kazakhstan. AD - Department of Agronomy, Breeding and Biotechnology, Kazakh National Agrarian Research University, Almaty 050010, Kazakhstan. FAU - Zhumaliyeva, Gaziza AU - Zhumaliyeva G AD - Plant Biotechnology Laboratory, Kazakh Research Institute of Agriculture and Plant Growing, Almalybak 040909, Kazakhstan. FAU - Didorenko, Svetlana AU - Didorenko S AD - Oilseed Crops Laboratory, Kazakh Research Institute of Agriculture and Plant Growing, Almalybak 040909, Kazakhstan. FAU - Kushanova, Rystay AU - Kushanova R AUID- ORCID: 0000-0001-6003-9298 AD - Oilseed Crops Laboratory, Kazakh Research Institute of Agriculture and Plant Growing, Almalybak 040909, Kazakhstan. FAU - Zinchenko, Alyona AU - Zinchenko A AD - Oil Crops Department, Zarechnoye Experimental Station, Zarechnoye 111108, Kazakhstan. FAU - Bastaubayeva, Sholpan AU - Bastaubayeva S AUID- ORCID: 0000-0003-2588-5880 AD - Plant Biotechnology Laboratory, Kazakh Research Institute of Agriculture and Plant Growing, Almalybak 040909, Kazakhstan. FAU - Ahmed, Zaheer AU - Ahmed Z AD - Department of Plant Breeding and Genetics, University of Agriculture, Faisalabad 38000, Pakistan. FAU - Yerzhebayeva, Raushan AU - Yerzhebayeva R AUID- ORCID: 0000-0003-4585-8505 AD - Plant Biotechnology Laboratory, Kazakh Research Institute of Agriculture and Plant Growing, Almalybak 040909, Kazakhstan. LA - eng GR - BR22885857/Ministry of Agriculture of the Republic of Kazakhstan/ PT - Journal Article DEP - 20261003 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705672 OTO - NOTNLM OT - E loci OT - adaptation OT - early maturity OT - high latitudes OT - introgression OT - marker-assisted selection OT - photoperiod response OT - soybean COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/03 CRDT- 2026/10/27 10:42 PHST- 2026/09/10 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - life16101663 [pii] AID - life-16-01663 [pii] AID - 10.3390/life16101663 [doi] PST - epublish SO - Life (Basel). 2026 Oct 3;16(10):1663. doi: 10.3390/life16101663. PMID- 42942246 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 2 TI - Peptides in Aesthetic Dermatology: Classification, Fibroblast-Centered Mechanisms, Delivery Technologies, and Clinical Evidence. LID - 10.3390/life16101660 [doi] LID - 1660 AB - Peptides used in aesthetic dermatology differ in molecular identity, biological target, formulation, and route of administration. This critical narrative review classifies these agents and examines their direct or indirect relationships with dermal fibroblasts, integrating mechanistic, delivery, and clinical evidence. Matrikine-like and copper-binding peptides influence matrix-related responses in experimental systems, whereas neuromodulatory and pigment-modulating peptides primarily act through other cellular targets. Oral collagen hydrolysates provide amino acids and circulating hydroxyproline-containing peptides with fibroblast activity demonstrated mainly in preclinical models. Lipidation, encapsulation, and microneedles can modify exposure, but delivery-system effects complicate attribution to the peptide. Controlled human studies report improvements in selected skin endpoints, including wrinkles, hydration, elasticity, dermal density, and pigmentation; however, many trials are limited by small samples, short follow-up, industry sponsorship, or multi-ingredient formulations. Current evidence supports product-specific interpretation rather than interchangeability across peptide classes or administration routes. It does not establish universal purity thresholds, commercial shelf lives, or safety of degraded formulations. Future studies should connect characterized composition and verified exposure with cell-specific mechanisms, objective clinical outcomes, and route-appropriate safety assessment. FAU - Yi, Kyu-Ho AU - Yi KH AUID- ORCID: 0000-0001-5572-1364 AD - Department of Oral Biology, Yonsei University College of Dentistry, Seoul 03722, Republic of Korea. FAU - Kim, Hong Seok AU - Kim HS AUID- ORCID: 0009-0000-1156-7393 AD - VOS Dermatology Clinic, Seoul 06021, Republic of Korea. FAU - Lee, Han Earl AU - Lee HE AD - Re & Re Plastic Surgery Clinic, Seoul 06038, Republic of Korea. FAU - Choi, Won Seok AU - Choi WS AD - V Plastic Surgery, Daegu 41940, Republic of Korea. FAU - Kim, Jin-Hyun AU - Kim JH AD - You and I Clinic, Seoul 06123, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20261002 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705772 OTO - NOTNLM OT - GHK-Cu OT - aesthetic dermatology OT - bioactive peptides OT - collagen peptides OT - cosmeceuticals OT - dermal fibroblasts OT - matrikines OT - palmitoyl pentapeptide-4 OT - skin aging OT - transdermal delivery COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/02 CRDT- 2026/10/27 10:42 PHST- 2026/08/30 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/02 00:00 [pmc-release] AID - life16101660 [pii] AID - life-16-01660 [pii] AID - 10.3390/life16101660 [doi] PST - epublish SO - Life (Basel). 2026 Oct 2;16(10):1660. doi: 10.3390/life16101660. PMID- 42942242 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Oct 1 TI - Metabolic Dysfunction-Associated Steatotic Liver Disease and Steatohepatitis (MASLD/MASH) and Cardiovascular Risk: Emerging Therapeutic Strategies at the Interface of Liver and Cardiometabolic Disease. LID - 10.3390/life16101656 [doi] LID - 1656 AB - Metabolic dysfunction-associated steatotic liver disease (MASLD) and its progressive form, metabolic dysfunction-associated steatohepatitis (MASH), are increasingly recognized as systemic cardiometabolic disorders rather than isolated liver diseases. Cardiovascular disease (CVD) represents the leading cause of mortality in patients with MASLD/MASH, highlighting the need for therapeutic strategies that address both hepatic and cardiovascular risk. MASLD/MASH and CVD are connected through a network of interdependent mechanisms, including adipose tissue dysfunction, insulin resistance, altered insulin-glucagon signaling, atherogenic dyslipidemia, lipotoxicity, mitochondrial and endoplasmic reticulum stress, innate immune activation, chronic inflammation, endothelial dysfunction, thrombogenic signaling, and dysregulated hepatokine and adipokine pathways. These mechanisms provide potential therapeutic entry points across the liver-adipose tissue-vascular axis. Lifestyle modification and conventional cardiometabolic therapies remain foundational, while several pharmacological agents increasingly target specific components of this network. We summarize the mechanistic rationale and clinical evidence for emerging therapies, including glucagon-like peptide-1 receptor agonists, dual incretin agonists, fibroblast growth factor 21 analogs, thyroid hormone receptor-beta agonists, peroxisome proliferator-activated receptor agonists, and agents targeting de novo lipogenesis. We further discuss why residual metabolic, inflammatory, and fibrogenic activity may limit monotherapy and how mechanism-based combination therapy could improve efficacy while mitigating treatment-related metabolic liabilities. Finally, we outline future priorities, including biomarker-guided patient selection, longitudinal noninvasive monitoring, integrated hepatic-cardiovascular trial endpoints, adaptive combination strategies, and development of therapies targeting immune, fibrogenic, and inter-organ signaling pathways. FAU - Boutari, Chrysoula AU - Boutari C AUID- ORCID: 0000-0002-0053-2440 AD - 4th Department of Internal Medicine, Hippokration General Hospital, Aristotle University of Thessaloniki, 54642 Thessaloniki, Greece. FAU - Charalampidis, Konstantinos AU - Charalampidis K AD - 4th Department of Internal Medicine, Hippokration General Hospital, Aristotle University of Thessaloniki, 54642 Thessaloniki, Greece. FAU - Sideras, Lazaros AU - Sideras L AUID- ORCID: 0000-0002-1984-0697 AD - 4th Department of Internal Medicine, Hippokration General Hospital, Aristotle University of Thessaloniki, 54642 Thessaloniki, Greece. FAU - Sinakos, Emmanouil AU - Sinakos E AUID- ORCID: 0000-0003-0923-050X AD - 4th Department of Internal Medicine, Hippokration General Hospital, Aristotle University of Thessaloniki, 54642 Thessaloniki, Greece. FAU - Goulis, Ioannis AU - Goulis I AD - 4th Department of Internal Medicine, Hippokration General Hospital, Aristotle University of Thessaloniki, 54642 Thessaloniki, Greece. LA - eng PT - Journal Article PT - Review DEP - 20261001 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705867 OTO - NOTNLM OT - FGF 21 analogs OT - GLP-1 receptor agonists OT - MASH OT - MASLD OT - PPAR agonists OT - cardiovascular risk OT - dual incretin agonists OT - thyroid hormone receptor-beta agonists COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:42 PHST- 2026/07/17 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - life16101656 [pii] AID - life-16-01656 [pii] AID - 10.3390/life16101656 [doi] PST - epublish SO - Life (Basel). 2026 Oct 1;16(10):1656. doi: 10.3390/life16101656. PMID- 42942238 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Sep 30 TI - Genome-Wide Discovery and Evaluation of Kazakh Ancestry-Informative Markers. LID - 10.3390/life16101652 [doi] LID - 1652 AB - Kazakh populations occupy an important position within the genetic landscape of Central Asia and Inner Eurasia, yet compact ancestry-informative marker panels evaluated for Kazakh-related ancestry remain limited. We analyzed genome-wide single-nucleotide polymorphism data from 624 Kazakh individuals together with data from external reference populations. After harmonization, the discovery dataset comprised 1607 individuals from 41 population groups and 80,766 shared autosomal variants. Candidate ancestry-informative markers were ranked using allele-frequency differentiation and informativeness for assignment, followed by linkage disequilibrium filtering. The final panel comprised 385 markers. Because marker availability differed across external datasets, transferability assessment in two independent Kazakh cohorts (n = 224 and n = 132) was performed using the 341-marker subset shared across the internal dataset and both external cohorts. Principal component analysis showed that both external Kazakh cohorts overlapped substantially with the internal Kazakh cluster. In nearest-centroid analysis, 94.6% of internal Kazakh individuals and 92.0% and 91.7% of individuals from the two external Kazakh cohorts, respectively, were assigned as Kazakh-related. Among non-Kazakh reference individuals used in the discovery framework, 4.1% were assigned as Kazakh-related; this internal, selection-informed estimate was mainly driven by genetically close Central Asian and Inner Eurasian populations. These findings support the transferability of the shared marker subset across independent Kazakh datasets, while also showing limited discrimination between Kazakhs and several closely related neighboring populations. FAU - Zhaniyazov, Zhassulan AU - Zhaniyazov Z AUID- ORCID: 0000-0002-0893-8162 AD - Institute of Genetics and Physiology, CS MSHE RK, Almaty 050060, Kazakhstan. AD - Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan. AD - K. Turysov Institute of Geology and Oil and Gas Engineering, Satbayev University, Almaty 050013, Kazakhstan. FAU - Kulatayeva, Akmaral AU - Kulatayeva A AUID- ORCID: 0009-0007-1046-0878 AD - Institute of Genetics and Physiology, CS MSHE RK, Almaty 050060, Kazakhstan. AD - K. Turysov Institute of Geology and Oil and Gas Engineering, Satbayev University, Almaty 050013, Kazakhstan. FAU - Altynova, Nazym AU - Altynova N AUID- ORCID: 0000-0003-3239-2822 AD - Institute of Genetics and Physiology, CS MSHE RK, Almaty 050060, Kazakhstan. FAU - Zhaksybay, Zhuldyz AU - Zhaksybay Z AD - Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan. AD - K. Turysov Institute of Geology and Oil and Gas Engineering, Satbayev University, Almaty 050013, Kazakhstan. FAU - Baidullayeva, Assel AU - Baidullayeva A AUID- ORCID: 0009-0004-8348-9127 AD - K. Turysov Institute of Geology and Oil and Gas Engineering, Satbayev University, Almaty 050013, Kazakhstan. FAU - Mustafayeva, Aikorkem AU - Mustafayeva A AUID- ORCID: 0000-0001-9255-3777 AD - Institute of Genetics and Physiology, CS MSHE RK, Almaty 050060, Kazakhstan. AD - Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan. FAU - Iksan, Olzhas AU - Iksan O AUID- ORCID: 0000-0002-2960-695X AD - Institute of Genetics and Physiology, CS MSHE RK, Almaty 050060, Kazakhstan. AD - Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan. FAU - Razali, Rozaimi AU - Razali R AUID- ORCID: 0000-0002-8996-3975 AD - Department of Biomedical Sciences, Qatar University, Doha 2713, Qatar. FAU - Djansugurova, Leyla AU - Djansugurova L AUID- ORCID: 0000-0002-6745-9903 AD - Institute of Genetics and Physiology, CS MSHE RK, Almaty 050060, Kazakhstan. AD - Faculty of Biology and Biotechnology, Al-Farabi Kazakh National University, Almaty 050040, Kazakhstan. LA - eng GR - AP23489627/the Science Committee of the Ministry of Science and Higher Education of the Republic of Kazakhstan/ PT - Journal Article DEP - 20260930 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705973 OTO - NOTNLM OT - Central Asia OT - Kazakh population OT - ancestry-informative markers OT - genetic ancestry OT - nearest-centroid assignment OT - population genetics OT - single-nucleotide polymorphisms OT - transferability assessment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:42 PHST- 2026/08/04 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - life16101652 [pii] AID - life-16-01652 [pii] AID - 10.3390/life16101652 [doi] PST - epublish SO - Life (Basel). 2026 Sep 30;16(10):1652. doi: 10.3390/life16101652. PMID- 42942230 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Sep 30 TI - Mandible-Specific Noise Reduction in Pediatric Dental CBCT with Simulated Noise: Search Window Selection for the Fast Non-Local Means Algorithm. LID - 10.3390/life16101645 [doi] LID - 1645 AB - Accurate assessment of the pediatric mandible using dental cone-beam computed tomography (CBCT) is crucial despite radiation exposure concerns. The fast non-local means (FNLM) algorithm reduces noise in CBCT images, but its performance depends on parameter selection. This study compared FNLM search window sizes for pediatric mandibular CBCT images under simulated noise degradation. Mandibular CBCT images were acquired from 17 pediatric datasets in the MMDental database. Noise degradation was simulated by adding Poisson and Gaussian noise (sigma = 0.05). The FNLM algorithm was applied using search windows from 3 x 3 to 21 x 21 pixels and image quality was assessed using noise, similarity, and no-reference metrics and visual evaluation. Most metrics changed progressively with increasing search window size, with smaller changes beyond 7 x 7, and BRISQUE reached its lowest value at 7 x 7. Visual assessment showed noise reduction but decreased sharpness of anatomical boundaries with larger search windows. Among the evaluated conditions, the 7 x 7 search window provided the best compromise between noise reduction and structural preservation. These findings indicate that appropriate search window selection is important for FNLM-based noise reduction in pediatric mandibular CBCT images and should be interpreted within the simulated conditions of this study. FAU - Kim, Hee-Seo AU - Kim HS AUID- ORCID: 0009-0008-2910-3560 AD - Department of Dental Hygiene, Gachon University, 191, Hambangmoe-ro, Yeonsu-gu, Incheon 21936, Republic of Korea. FAU - Kim, Hajin AU - Kim H AD - Department of Health Science, General Graduate School of Gachon University, 191, Hambangmoe-ro, Yeonsu-gu, Incheon 21936, Republic of Korea. FAU - Kwon, Young-Eun AU - Kwon YE AUID- ORCID: 0000-0001-5795-6968 AD - Department of Oral and Maxillofacial Radiology, School of Dentistry, IHBR, Kyungpook National University, 2175, Dalgubeol-daero, Jung-gu, Daegu 41940, Republic of Korea. FAU - Kim, Ji-Youn AU - Kim JY AUID- ORCID: 0000-0003-3110-6468 AD - Department of Dental Hygiene, Gachon University, 191, Hambangmoe-ro, Yeonsu-gu, Incheon 21936, Republic of Korea. LA - eng GR - RS-2025-23523111/National Research Foundation of Korea/ GR - RS-2026-25493621/National Research Foundation of Korea/ PT - Journal Article DEP - 20260930 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706128 OTO - NOTNLM OT - cone-beam computed tomography OT - fast non-local means algorithm OT - mandible OT - noise reduction OT - pediatric patients OT - search window COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:42 PHST- 2026/08/04 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:42 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - life16101645 [pii] AID - life-16-01645 [pii] AID - 10.3390/life16101645 [doi] PST - epublish SO - Life (Basel). 2026 Sep 30;16(10):1645. doi: 10.3390/life16101645. PMID- 42942227 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Sep 30 TI - Metabolomic Profiling of Gestational Diabetes Mellitus and Its Subtypes Among Chinese Pregnant Women. LID - 10.3390/life16101641 [doi] LID - 1641 AB - Background: This study aimed to explore first-trimester metabolic profiles associated with gestational diabetes mellitus (GDM) and its glycemic subtypes. Methods: This nested case-control study included 90 women with GDM, comprising 30 with isolated fasting hyperglycemia (IFH), 30 with isolated post-load hyperglycemia (IPH), and 30 with combined hyperglycemia (CH), together with 30 women with normal glucose tolerance (NGT). Untargeted UHPLC-MS metabolomic profiling was performed on fasting plasma samples collected during the first trimester. Exploratory metabolic features were compared between GDM or its subtypes and NGT, and Spearman correlation analyses were performed to examine associations between candidate metabolic features and maternal fasting blood glucose (FBG), BMI, thyroid-related parameters, parity, education, and gestational weight gain. Exploratory pathway analyses were conducted to characterize potentially related metabolic processes. Results: At the exploratory threshold of VIP > 1 and nominal p < 0.05, 107 candidate metabolic features were observed in the GDM vs. NGT comparison, with 89, 76, and 123 candidate features observed in the IFH vs. NGT, IPH vs. NGT, and CH vs. NGT comparisons, respectively. These candidate features were mainly annotated as organoheterocyclic compounds and lipids and lipid-like molecules, with differences in chemical-class distributions across subtype comparisons. Organosulfur-related annotations were observed among the IFH candidate features, whereas alkaloid-related annotations were observed among the CH candidate features. No individual feature remained statistically significant after multiple-testing correction. Correlation analyses identified FDR-adjusted associations between selected candidate metabolic features and maternal FBG, BMI, and thyroid-related parameters. Exploratory pathway mapping highlighted processes related to lipid, amino acid, carbohydrate, and nucleotide metabolism. Conclusions: This exploratory study suggests that first-trimester plasma metabolic patterns may differ among women who subsequently develop different glycemic subtypes of GDM. The observed candidate features, clinical correlations, and pathway-level patterns provide hypotheses regarding metabolic heterogeneity in GDM but should not be considered validated biomarkers or subtype-specific metabolic signatures. Further studies using targeted metabolomics and larger independent cohorts are needed to confirm these observations. FAU - Alifu, Xialidan AU - Alifu X AUID- ORCID: 0000-0002-0800-5995 AD - School of Basic Medicine, Xinjiang Key Laboratory of Molecular Biology for Endemic Diseases, Xinjiang Medical University, Urumqi 830017, China. AD - Department of Epidemiology & Health Statistics, School of Public Health, School of Medicine, Zhejiang University, Hangzhou 310058, China. FAU - Qiu, Yiwen AU - Qiu Y AD - Department of Epidemiology & Health Statistics, School of Public Health, School of Medicine, Zhejiang University, Hangzhou 310058, China. AD - Department of Public Health, and Department of Anesthesiology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China. FAU - Wang, Boya AU - Wang B AD - Department of Epidemiology & Health Statistics, School of Public Health, School of Medicine, Zhejiang University, Hangzhou 310058, China. AD - Department of Public Health, and Department of Anesthesiology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China. FAU - Xu, Nuo AU - Xu N AD - Department of Epidemiology & Health Statistics, School of Public Health, School of Medicine, Zhejiang University, Hangzhou 310058, China. AD - Department of Public Health, and Department of Anesthesiology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China. FAU - Wang, Shuhui AU - Wang S AD - Department of Epidemiology & Health Statistics, School of Public Health, School of Medicine, Zhejiang University, Hangzhou 310058, China. AD - Department of Public Health, and Department of Anesthesiology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China. FAU - Cheng, Haoyue AU - Cheng H AUID- ORCID: 0000-0002-3538-0678 AD - Department of Epidemiology & Health Statistics, School of Public Health, School of Medicine, Zhejiang University, Hangzhou 310058, China. AD - Department of Public Health, and Department of Anesthesiology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China. FAU - Huang, Ye AU - Huang Y AD - Department of Epidemiology & Health Statistics, School of Public Health, School of Medicine, Zhejiang University, Hangzhou 310058, China. AD - Department of Public Health, and Department of Anesthesiology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China. FAU - Zhang, Libi AU - Zhang L AD - Department of Epidemiology & Health Statistics, School of Public Health, School of Medicine, Zhejiang University, Hangzhou 310058, China. AD - Department of Public Health, and Department of Anesthesiology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China. FAU - Ainiwan, Diliyaer AU - Ainiwan D AD - Department of Epidemiology & Health Statistics, School of Public Health, School of Medicine, Zhejiang University, Hangzhou 310058, China. AD - Department of Public Health, and Department of Anesthesiology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China. FAU - Zhou, Haibo AU - Zhou H AD - Department of Epidemiology & Health Statistics, School of Public Health, School of Medicine, Zhejiang University, Hangzhou 310058, China. AD - Department of Public Health, and Department of Anesthesiology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China. FAU - Liu, Hui AU - Liu H AD - Clinical Research Center, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China. FAU - Chen, Danqing AU - Chen D AD - Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China. FAU - Yu, Yunxian AU - Yu Y AUID- ORCID: 0000-0002-3394-9832 AD - Department of Epidemiology & Health Statistics, School of Public Health, School of Medicine, Zhejiang University, Hangzhou 310058, China. AD - Department of Public Health, and Department of Anesthesiology, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China. LA - eng GR - XJRC-2025-KJ-YJ-CXPT-110/the Talent Recruitment Project for Key Innovation Platforms (Bases) in Science and Technology under the Xinjiang Leading Talent Introduction Program/ GR - 2022YFC2703505, 2021YFC2701901/the National Key Research and Development Programme of China/ GR - ZDFY2021-4X104/the 4 +X Clinical Research Project of Women's Hospital, School of Medicine, Zhejiang University/ PT - Journal Article DEP - 20260930 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706523 OTO - NOTNLM OT - gestational diabetes mellitus OT - heterogeneous disease OT - thyroid function OT - untargeted metabolomics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:41 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - life16101641 [pii] AID - life-16-01641 [pii] AID - 10.3390/life16101641 [doi] PST - epublish SO - Life (Basel). 2026 Sep 30;16(10):1641. doi: 10.3390/life16101641. PMID- 42942223 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Sep 30 TI - Strategies for Selecting Reliable Reference-Gene Combinations Across 2D and 3D Cell Culture Models. LID - 10.3390/life16101637 [doi] LID - 1637 AB - Reliable RT-qPCR normalization in dynamic 2D/3D cell models requires more than identifying genes with low expression variability. In the present study, we evaluated 11 candidate reference genes in AML12 hepatocytes and 3T3-L1 adipocytes cultured under corresponding 2D and 3D conditions to determine whether conventional stability assessment was sufficient for selecting a reliable normalization combination. All candidates passed the initial screening in AML12 cells, whereas Actb, 18S, and Gapdh failed one or more predefined stability criteria in 3T3-L1 cells. geNorm pairwise variation indicated that two-gene normalization was sufficient in both models (V2/3 < 0.15). Nevertheless, linear mixed-effects analysis revealed a significant effect of culture condition on 9 of the 11 candidates in AML12 cells and on all 11 candidates in 3T3-L1 cells. Further analyses using PCA, hierarchical clustering, and BestKeeper-based correlation showed that high expression stability and strong inter-reference concordance coexisted with coordinated condition-dependent variation and associations with biologically regulated target genes. Consequently, some of the algorithmically top-ranked pairs did not exhibit the most favorable profile in terms of expression independence, while the choice of normalizer affected both the magnitude and the statistical interpretation of target-gene responses. Based on these findings, we propose a multistep, model-specific strategy that treats expression stability, inter-reference concordance, expression redundancy, condition dependence, and biological independence as distinct characteristics. This approach shifts the focus from selecting the "most stable" genes toward experimental validation of the reference-gene combination itself and of its impact on biological interpretation. FAU - Grigorova, Natalia AU - Grigorova N AUID- ORCID: 0000-0001-5749-7773 AD - Department of Pharmacology, Animal Physiology, Biochemistry and Chemistry, Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria. LA - eng GR - Scientific Project No. 06/2024 of the Faculty of Veterinary Medicine/Trakia University/ PT - Journal Article DEP - 20260930 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706404 OTO - NOTNLM OT - 2D and 3D cell culture OT - 3T3-L1 adipocytes OT - AML12 hepatocytes OT - RT-qPCR OT - candidate reference genes OT - cell line-specific effects OT - culture architecture OT - gene expression normalization COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:41 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - life16101637 [pii] AID - life-16-01637 [pii] AID - 10.3390/life16101637 [doi] PST - epublish SO - Life (Basel). 2026 Sep 30;16(10):1637. doi: 10.3390/life16101637. PMID- 42942221 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Sep 30 TI - Anti-Ascaris IgG Seroprevalence and ELISA Performance Among Adults in Three Regions of Kazakhstan. LID - 10.3390/life16101635 [doi] LID - 1635 AB - Regional surveys can help characterize adult exposure to Ascaris and assess the interpretation of different laboratory markers. This cross-sectional study examined 2001 volunteers from the Akmola, Pavlodar, and North Kazakhstan Regions during 2025-2026. Questionnaire data were collected, and paired serum and stool specimens were tested using the MVA GROUP IgG ELISA and Kato-Katz method. Associations with seropositivity were evaluated using multivariable logistic regression, and 300 selected sera were compared with the DRG Ascaris IgG ELISA. Overall seroprevalence was 28.0% (95% CI: 26.1-30.0%), with regional differences remaining after adjustment for age, sex, residence, and sampling year. Kato-Katz positivity was 8.4%, without a significant regional difference. ELISA sensitivity and specificity relative to Kato-Katz were 85.2% and 77.2%, respectively. Agreement with DRG was 95.0% (kappa = 0.900). These findings support further regional assessment of adult exposure. Agreement between ELISAs supports consistent antibody classification in the selected panel, while discordance with Kato-Katz requires separate interpretation of antibody detection and intestinal egg shedding. FAU - Khairullin, Berik M AU - Khairullin BM AD - Scientific Research and Production Center <> LLP, 1124, Accounting Quarter 096, Koksay Village, Irgeli Rural District, Karasay District, Almaty 040921, Kazakhstan. FAU - Orynbayev, Mukhit B AU - Orynbayev MB AUID- ORCID: 0000-0002-5882-4964 AD - Scientific Research and Production Center <> LLP, 1124, Accounting Quarter 096, Koksay Village, Irgeli Rural District, Karasay District, Almaty 040921, Kazakhstan. AD - LLP <> JSC <> of the Ministry of Health of the Republic of Kazakhstan, Gvardeysky 080409, Kazakhstan. FAU - Davlyatshin, Timur I AU - Davlyatshin TI AUID- ORCID: 0000-0002-1064-7081 AD - Scientific Research and Production Center <> LLP, 1124, Accounting Quarter 096, Koksay Village, Irgeli Rural District, Karasay District, Almaty 040921, Kazakhstan. FAU - Sultankulova, Kulyaisan T AU - Sultankulova KT AD - Scientific Research and Production Center <> LLP, 1124, Accounting Quarter 096, Koksay Village, Irgeli Rural District, Karasay District, Almaty 040921, Kazakhstan. AD - LLP <> JSC <> of the Ministry of Health of the Republic of Kazakhstan, Gvardeysky 080409, Kazakhstan. FAU - Tussipova, Aiganym A AU - Tussipova AA AUID- ORCID: 0000-0002-7767-0542 AD - Scientific Research and Production Center <> LLP, 1124, Accounting Quarter 096, Koksay Village, Irgeli Rural District, Karasay District, Almaty 040921, Kazakhstan. FAU - Barmak, Sabyrkhan M AU - Barmak SM AD - Scientific Research and Production Center <> LLP, 1124, Accounting Quarter 096, Koksay Village, Irgeli Rural District, Karasay District, Almaty 040921, Kazakhstan. FAU - Myrzakhmet, Asylzhan A AU - Myrzakhmet AA AUID- ORCID: 0000-0003-4107-5095 AD - Scientific Research and Production Center <> LLP, 1124, Accounting Quarter 096, Koksay Village, Irgeli Rural District, Karasay District, Almaty 040921, Kazakhstan. FAU - Tusipkanuly, Oralkhan AU - Tusipkanuly O AD - Scientific Research and Production Center <> LLP, 1124, Accounting Quarter 096, Koksay Village, Irgeli Rural District, Karasay District, Almaty 040921, Kazakhstan. FAU - Inkarbekov, Dulat A AU - Inkarbekov DA AD - Scientific Research and Production Center <> LLP, 1124, Accounting Quarter 096, Koksay Village, Irgeli Rural District, Karasay District, Almaty 040921, Kazakhstan. FAU - Davlyatshin, Timur T AU - Davlyatshin TT AD - Scientific Research and Production Center <> LLP, 1124, Accounting Quarter 096, Koksay Village, Irgeli Rural District, Karasay District, Almaty 040921, Kazakhstan. FAU - Dmitrovskiy, Andrey M AU - Dmitrovskiy AM AD - National Center for Biotechnology, Central Reference Laboratory, 14 Zhahanger St., Almaty 050054, Kazakhstan. FAU - Mukazhanova, Sandugash E AU - Mukazhanova SE AD - RSE <
>, Astana Z19Y8B0, Kazakhstan. FAU - Nusupbaeva, Gaukhar E AU - Nusupbaeva GE AD - RSE <
>, Astana Z19Y8B0, Kazakhstan. FAU - Namet, Aidar M AU - Namet AM AD - Scientific Research and Production Center <> LLP, 1124, Accounting Quarter 096, Koksay Village, Irgeli Rural District, Karasay District, Almaty 040921, Kazakhstan. LA - eng GR - The study was supported by program-targeted financing from the Science Committee of the Ministry of Science and Higher Education of the Republic of Kazakhstan (IRN BR28712927). "Scientific and technological support for biological safety of the Republic of/Science Committee of the Ministry of Science and Higher Education of the Republic of Kazakhstan/ PT - Journal Article DEP - 20260930 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705662 OTO - NOTNLM OT - Ascaris lumbricoides OT - ELISA OT - Kato-Katz OT - Kazakhstan OT - adults OT - ascariasis OT - seroprevalence COIS- The authors declare no apparent or potential conflicts of interest related to the publication of this article. The funder had no role in the study's design; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in deciding whether to submit the article for publication. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:41 PHST- 2026/09/07 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - life16101635 [pii] AID - life-16-01635 [pii] AID - 10.3390/life16101635 [doi] PST - epublish SO - Life (Basel). 2026 Sep 30;16(10):1635. doi: 10.3390/life16101635. PMID- 42942216 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Sep 29 TI - Changes in Functional Performance During and After a Supervised Exercise Programme in People with Multiple Sclerosis: A Secondary Uncontrolled Longitudinal Study. LID - 10.3390/life16101630 [doi] LID - 1630 AB - BACKGROUND: Exercise is clinically relevant for people with multiple sclerosis (PwMS), but participation in structured programmes is often intermittent. This exploratory study described changes in functional and exercise-related outcomes during and after a supervised exercise programme. METHODS: This secondary uncontrolled longitudinal analysis included 20 adults with MS who had three consecutive assessments: before (T1) and after (T2) a 12-week programme comprising two weekly 60-min supervised sessions, and after an approximately three-month period without supervised training. Outcomes included functional performance, gait parameters, fatigue and exercise-testing outcomes. Linear mixed-effects models were used as the main longitudinal analysis, with paired Wilcoxon tests as complementary comparisons. RESULTS: The median 2-Minute Walk Test distance increased from 121 m at baseline to 126 m after the programme and was 125 m at follow-up. The corresponding mixed-model estimate for T2 versus T1 was 5.00 m (95% CI: -0.29 to 10.29; p = 0.063), whereas the paired comparison yielded p = 0.046. Achieved workload, VO(2) estimated using the FRIEND equation, and percentage of predicted VO(2) achieved showed numerical increases after the programme, but these changes were not statistically significant. Most other outcomes remained broadly stable, with heterogeneous individual trajectories. CONCLUSIONS: Modest within-group changes were observed in selected walking and exercise-test-derived outcomes, but the findings were not consistently supported across statistical approaches. Given the small sample and absence of a control group, the results should be considered exploratory and cannot establish programme effectiveness or programme-related preservation of function. FAU - Tarditi, Filippo AU - Tarditi F AUID- ORCID: 0009-0008-2679-2196 AD - Department of Medical Sciences, University of Torino, 10126 Torino, Italy. FAU - Beratto, Luca AU - Beratto L AUID- ORCID: 0000-0002-8479-0067 AD - Department of Medical Sciences, University of Torino, 10126 Torino, Italy. AD - NeuroMuscular Function|Research Group, School of Exercise and Sport Sciences (SUISM), University of Torino, 10126 Torino, Italy. FAU - Borrelli, Francesco AU - Borrelli F AUID- ORCID: 0009-0003-6938-0806 AD - Department of Medical Sciences, University of Torino, 10126 Torino, Italy. FAU - Koumantakis, Emanuele AU - Koumantakis E AUID- ORCID: 0000-0001-6994-8375 AD - Center for Biostatistics, Epidemiology, and Public Health, Department of Clinical and Biological Sciences, University of Torino, 10126 Torino, Italy. AD - Cancer Epidemiology Unit, Citta della Salute e della Scienza di Torino University Hospital, 10126 Torino, Italy. FAU - Actis Dato, Giulia AU - Actis Dato G AUID- ORCID: 0009-0002-2798-3564 AD - Department of Medical Sciences, University of Torino, 10126 Torino, Italy. FAU - Rossi, Gaetano AU - Rossi G AD - Department of Medical Sciences, University of Torino, 10126 Torino, Italy. FAU - Bressy, Lara AU - Bressy L AUID- ORCID: 0009-0004-2452-122X AD - NeuroMuscular Function|Research Group, School of Exercise and Sport Sciences (SUISM), University of Torino, 10126 Torino, Italy. FAU - Turiano, Elena AU - Turiano E AD - 160CM APS, 10126 Torino, Italy. FAU - Rainoldi, Alberto AU - Rainoldi A AUID- ORCID: 0000-0003-4553-2898 AD - Department of Medical Sciences, University of Torino, 10126 Torino, Italy. AD - NeuroMuscular Function|Research Group, School of Exercise and Sport Sciences (SUISM), University of Torino, 10126 Torino, Italy. FAU - Benso, Andrea Silvio AU - Benso AS AD - Department of Medical Sciences, University of Torino, 10126 Torino, Italy. AD - Division of Endocrinology, Diabetology and Metabolism, Department of Medical Sciences, University of Torino, 10126 Torino, Italy. FAU - Mulasso, Anna AU - Mulasso A AUID- ORCID: 0000-0002-9092-2994 AD - Department of Medical Sciences, University of Torino, 10126 Torino, Italy. AD - NeuroMuscular Function|Research Group, School of Exercise and Sport Sciences (SUISM), University of Torino, 10126 Torino, Italy. LA - eng GR - 160CM APS/ PT - Journal Article DEP - 20260929 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706077 OTO - NOTNLM OT - exercise capacity OT - follow-up OT - functional performance OT - high-intensity interval training OT - multiple sclerosis OT - physical exercise OT - resistance training OT - supervised exercise COIS- The authors declare no conflicts of interest. Participants were recruited among members of 160CM APS. Beyond providing financial support and representing the source population from which participants were recruited, 160CM APS had no role in the study design, participant eligibility assessment or enrolment procedures, data collection, data management, statistical analyses, interpretation of the results, writing of the manuscript, or decision to publish. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/29 CRDT- 2026/10/27 10:41 PHST- 2026/07/27 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/09/29 00:00 [pmc-release] AID - life16101630 [pii] AID - life-16-01630 [pii] AID - 10.3390/life16101630 [doi] PST - epublish SO - Life (Basel). 2026 Sep 29;16(10):1630. doi: 10.3390/life16101630. PMID- 42942215 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Sep 29 TI - Fluoxetine Is Associated with Reduced Cell Viability and Decreased pERK1/2 Immunoreactivity in Ishikawa Endometrial Cancer Cells In Vitro: An Exploratory Study. LID - 10.3390/life16101629 [doi] LID - 1629 AB - Selective serotonin reuptake inhibitors are widely prescribed in oncology, yet their direct effects on endometrial cancer cell fate remain unclear. This study examined whether fluoxetine, at concentrations referenced to reported plasma exposure ranges, alters viability, cell death phenotype, DNA integrity and extracellular signal-regulated kinase 1/2 (ERK1/2) signalling. Ishikawa endometrial cancer cells and normal human dermal fibroblasts (HDFs) were exposed to fluoxetine (100-300 ng/mL) for a single exposure period of 48 h. Metabolic viability was assessed by MTT assay, cell death phenotypes by annexin V/propidium iodide (PI) flow cytometry, DNA fragmentation by TUNEL, and total and phosphorylated ERK1/2 (pERK1/2) by immunofluorescence; all experiments were performed in three independent experiments (n = 3), and per-experiment means were used as the unit of analysis. Fluoxetine reduced metabolic viability in Ishikawa cells, with significant reductions detected at 100 and 300 ng/mL, whereas HDF viability remained largely preserved. Flow cytometry showed predominantly viable populations in both cell types, with low early apoptotic, late apoptotic and annexin V(-)/PI(+) fractions; no statistically significant treatment-related differences were detected in these cell death-associated populations, although analysis at a single 48 h endpoint cannot exclude earlier transient changes. TUNEL positivity showed a concentration-related numerical increase in Ishikawa cells, reaching statistical significance at 300 ng/mL, while remaining low in HDF cells. pERK1/2 immunoreactivity was significantly decreased at 300 ng/mL in both Ishikawa and HDF cells without a comparable change in total ERK1/2 immunoreactivity; because this readout was obtained by immunofluorescence only and was not validated by Western blotting, it is reported as a change in immunoreactivity rather than as confirmed pathway inhibition. These exploratory in vitro findings indicate that fluoxetine exposure is associated with reduced viability, DNA fragmentation and decreased pERK1/2 immunoreactivity in Ishikawa cells; the limited injury in HDF cells reflects a general non-malignant comparator response rather than cancer-specific selectivity. The findings are hypothesis-generating and require confirmation by protein- and transcript-level validation, pathway modulation experiments and additional malignant and non-malignant cell models. FAU - Ari, Neziha Senem AU - Ari NS AUID- ORCID: 0000-0003-2926-6892 AD - Department of Histology and Embryology, Faculty of Medicine, Kutahya Health Sciences University, 43100 Kutahya, Turkiye. FAU - Cakir Gundogdu, Ayse AU - Cakir Gundogdu A AUID- ORCID: 0000-0002-2466-9417 AD - Department of Histology and Embryology, Faculty of Medicine, Kutahya Health Sciences University, 43100 Kutahya, Turkiye. FAU - Meric, Neslihan AU - Meric N AUID- ORCID: 0000-0002-2878-5052 AD - Department of Molecular Biology and Genetics, Faculty of Engineering and Life Sciences, Kutahya Health Sciences University, 43100 Kutahya, Turkiye. FAU - Ozatik, Orhan AU - Ozatik O AD - Department of Histology and Embryology, Faculty of Medicine, Kutahya Health Sciences University, 43100 Kutahya, Turkiye. LA - eng GR - TSA-2023-149/Kutahya Saglik Bilimleri Universitesi/ PT - Journal Article DEP - 20260929 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706076 OTO - NOTNLM OT - ERK1/2 phosphorylation OT - Ishikawa cell line OT - MAPK/ERK pathway OT - TUNEL OT - annexin V/propidium iodide OT - drug repurposing OT - endometrial carcinoma OT - exploratory study OT - fluoxetine OT - in vitro cytotoxicity OT - selective serotonin reuptake inhibitor COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/29 CRDT- 2026/10/27 10:41 PHST- 2026/08/13 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/09/29 00:00 [pmc-release] AID - life16101629 [pii] AID - life-16-01629 [pii] AID - 10.3390/life16101629 [doi] PST - epublish SO - Life (Basel). 2026 Sep 29;16(10):1629. doi: 10.3390/life16101629. PMID- 42942212 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Sep 28 TI - One Pressure, Many Targets: Left Ventricular Filling Pressure as a Candidate Effect Modifier for Non-Invasive Ventilation in Acute Coronary Syndromes. LID - 10.3390/life16101626 [doi] LID - 1626 AB - Non-invasive ventilation relieves acute cardiogenic pulmonary edema and reduces intubation. Whether it saves lives is disputed; restricting the pooled record to the least biased trials leaves neither endpoint significant. My hypothesis: raising pleural pressure subtracts venous return; whether that subtraction is banked as unloading or paid as lost output depends on preload responsiveness, for which left ventricular filling pressure is a candidate effect modifier and not a measure. One radiographic label may contain two gas-exchange phenotypes, proposed for groups, not patients: carbon dioxide accumulates far more slowly than an ischemia-driven presentation floods the alveolus, and the remodeled ventricle arrives hypercapnic. Applied pressure inflates flooded air spaces without removing lung water, lowers left ventricular transmural pressure, and reaches two cardiac submechanisms. The left ventricular arm follows the sign rule; the renal arm follows it only through volume status; and in right ventricular infarction, the recorded filling pressure misleads, while the pulmonary arm does not reverse. In acute coronary syndromes, the first prediction fails if transmural pressure falls no more in the elevated than in the normal stratum, or if stroke volume index does not fall in the latter. The outer boundary is the requirement for emergency reperfusion, a limit inferred rather than measured directly. FAU - Tomala, Marek AU - Tomala M AUID- ORCID: 0000-0001-7882-9255 AD - Faculty of Medicine and Health Sciences, University of Applied Sciences in Nowy Sacz, 33-300 Nowy Sacz, Poland. AD - Center for Invasive Cardiology, Electrotherapy, and Angiology, Clinical Research Center Intercard, Al. Beliny-Prazmowskiego 60/4, 31-514 Krakow, Poland. LA - eng PT - Journal Article PT - Review DEP - 20260928 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705974 OTO - NOTNLM OT - acute coronary syndrome OT - alveolar recruitment OT - cardiogenic pulmonary edema OT - continuous positive airway pressure OT - heart-lung interaction OT - ischemic mitral regurgitation OT - non-invasive ventilation OT - transmural pressure OT - ventricular interdependence COIS- The author declares no financial or commercial interests. One intellectual relationship is disclosed in the text rather than here: the trial-level meta-regression cited at several points is the author's own, and Section 3, Section 5 and Section 8 state how it is used. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/28 CRDT- 2026/10/27 10:41 PHST- 2026/09/01 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - life16101626 [pii] AID - life-16-01626 [pii] AID - 10.3390/life16101626 [doi] PST - epublish SO - Life (Basel). 2026 Sep 28;16(10):1626. doi: 10.3390/life16101626. PMID- 42942207 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Sep 27 TI - Effects of Wheatgrass Juice Supplementation in Common Carp (Cyprinus carpio): Growth Performance, Biochemical Profile, Oxidative Status, and Cultivable Intestinal Bacteria. LID - 10.3390/life16101619 [doi] LID - 1619 AB - Aquaculture faces numerous challenges related to fish health. To explore new solutions, the objective of this study was to test the effects of wheatgrass juice (WGJ) as feed supplement on growth performance, blood biochemical profile, bacteriological balance, and oxidative stress in common carp (Cyprinus carpio). Carp fingerlings (8.8 g initial body weight) were fed diets containing 4% or 8% WGJ in a recirculating aquaculture system for eight weeks. Most growth performance parameters did not differ significantly among dietary treatments, although the viscerosomatic index (VSI) was significantly lower in WGJ-supplemented groups. WGJ supplementation was associated with changes in oxidative-status parameters, including increased antioxidant enzyme activities and reduced malondialdehyde levels, particularly at the 8% inclusion level. Blood biochemical parameters showed no evidence of adverse effects on liver or kidney function. Total protein, globulin, and albumin did not differ significantly among treatments. Microbiological examination highlighted a decrease in pathogenic bacteria, including sulfite-reducing clostridia, with a 4% WGJ diet showing the most pronounced inhibitory effects. These results indicate that WGJ supplementation may influence selected biochemical, oxidative-status, and cultivable intestinal bacterial parameters in common carp. Further studies are required to determine the underlying mechanisms and the practical relevance of these effects. FAU - Adrian, Balbarau AU - Adrian B AD - Faculty of Veterinary Medicine, "Ion Ionescu de la Brad" Iasi University of Life Sciences, Aleea Mihail Sadoveanu nr. 8, 700489 Iasi, Romania. FAU - Barbacariu, Cristian-Alin AU - Barbacariu CA AUID- ORCID: 0000-0002-7784-3001 AD - Research and Development Station for Aquaculture and Aquatic Ecology, "Alexandru Ioan Cuza" University, Carol I, 20A, 700505 Iasi, Romania. FAU - Dirvariu, Lenuta AU - Dirvariu L AD - Research and Development Station for Aquaculture and Aquatic Ecology, "Alexandru Ioan Cuza" University, Carol I, 20A, 700505 Iasi, Romania. FAU - Serban, Dana Andreea AU - Serban DA AUID- ORCID: 0000-0003-4750-7100 AD - Research and Development Station for Aquaculture and Aquatic Ecology, "Alexandru Ioan Cuza" University, Carol I, 20A, 700505 Iasi, Romania. FAU - Burducea, Marian AU - Burducea M AUID- ORCID: 0000-0002-6142-438X AD - Research and Development Station for Aquaculture and Aquatic Ecology, "Alexandru Ioan Cuza" University, Carol I, 20A, 700505 Iasi, Romania. FAU - Dumitru, Gabriela AU - Dumitru G AD - Faculty of Biology, "Alexandru Ioan Cuza" University, Carol I, 20A, 700505 Iasi, Romania. FAU - Todirascu-Ciornea, Elena AU - Todirascu-Ciornea E AD - Faculty of Biology, "Alexandru Ioan Cuza" University, Carol I, 20A, 700505 Iasi, Romania. FAU - Mihaela, Rimbu Cristina AU - Mihaela RC AUID- ORCID: 0000-0003-0982-0040 AD - Faculty of Veterinary Medicine, "Ion Ionescu de la Brad" Iasi University of Life Sciences, Aleea Mihail Sadoveanu nr. 8, 700489 Iasi, Romania. FAU - Dumitru, Acatrinei AU - Dumitru A AD - Faculty of Veterinary Medicine, "Ion Ionescu de la Brad" Iasi University of Life Sciences, Aleea Mihail Sadoveanu nr. 8, 700489 Iasi, Romania. FAU - Liviu, Miron AU - Liviu M AD - Faculty of Veterinary Medicine, "Ion Ionescu de la Brad" Iasi University of Life Sciences, Aleea Mihail Sadoveanu nr. 8, 700489 Iasi, Romania. LA - eng GR - ROMD00578/EcoFishFarm Lead Partner: Moldova State University (in partnership with the Institute of Zoology)Program: Interreg VI-A NEXT Romania-Republic of Moldova Programme 2021-2027 (Priority 1 - Green Communities)Timeline: June 24, 2025 - December 23, 2026Funding/ PT - Journal Article DEP - 20260927 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706233 OTO - NOTNLM OT - bacteriological OT - biochemical blood parameters OT - carp fingerlings (Cyprinus carpio) OT - clinical health OT - oxidative stress OT - wheatgrass juice COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/27 CRDT- 2026/10/27 10:41 PHST- 2026/07/31 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - life16101619 [pii] AID - life-16-01619 [pii] AID - 10.3390/life16101619 [doi] PST - epublish SO - Life (Basel). 2026 Sep 27;16(10):1619. doi: 10.3390/life16101619. PMID- 42942204 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Sep 26 TI - Development and Application of gSSRs Markers in Macadamia from Genomic Sequences. LID - 10.3390/life16101618 [doi] LID - 1618 AB - Macadamia (Macadamia spp.) is an economically important nut crop worldwide, but genomic simple sequence repeat (SSR) markers remain limited, especially for germplasm from South China, hindering comprehensive genetic diversity assessment and population structure analysis. In this study, we developed and characterized gSSR markers based on leaf genomic sequence data. A total of 76 primer pairs were initially designed, of which 65 were successfully amplified, and 18 highly polymorphic markers were selected for further analysis. Using these 18 gSSR markers, we genotyped 104 Macadamia individuals from 15 populations in South China. The markers detected a total of 118 alleles, with the number of alleles per locus ranging from 2.056 to 7.167 (mean = 2.911). The observed heterozygosity (H(o)) and expected heterozygosity (H(e)) varied from 0.556 to 0.697 and from 0.389 to 0.669, respectively. Analysis of molecular variance (AMOVA) revealed significant genetic differentiation among populations (F(ST) = 0.304, p < 0.01), with 16.39% of the variation occurring between populations and 83.61% within populations. Population structure analysis based on Structure, PCoA, and UPGMA clustering consistently divided the 15 populations into two distinct genetic groups. Relative migration analysis revealed substantial gene flow among the four populations examined in this study, with bidirectional relative migration rates all exceeding 0.7. One outlier locus (MacChr13-2) showing a positive selection signal was identified, suggesting potential adaptive divergence. These newly developed gSSR markers provide a valuable tool for genetic diversity assessment, germplasm management and cultivar improvement, and marker-assisted breeding of Macadamia. FAU - Cui, Can AU - Cui C AD - School of Horticulture and Food, Guangdong Eco-Engineering Polytechnic, Guangzhou 510520, China. FAU - Yang, Guifang AU - Yang G AD - School of Horticulture and Food, Guangdong Eco-Engineering Polytechnic, Guangzhou 510520, China. FAU - Liu, Yao AU - Liu Y AD - School of Horticulture and Food, Guangdong Eco-Engineering Polytechnic, Guangzhou 510520, China. FAU - Yu, Zijuan AU - Yu Z AD - School of Life Sciences, Sun Yat-sen University, Xingang Xi Lu 135, Guangzhou 510275, China. FAU - Yang, Guang AU - Yang G AD - School of Horticulture and Food, Guangdong Eco-Engineering Polytechnic, Guangzhou 510520, China. FAU - Ruan, Xiaoxian AU - Ruan X AD - School of Life Sciences, Sun Yat-sen University, Xingang Xi Lu 135, Guangzhou 510275, China. FAU - Zhang, Jinyun AU - Zhang J AD - School of Horticulture and Food, Guangdong Eco-Engineering Polytechnic, Guangzhou 510520, China. FAU - Wu, Shijun AU - Wu S AD - School of Horticulture and Food, Guangdong Eco-Engineering Polytechnic, Guangzhou 510520, China. LA - eng GR - 2023GCZX012/Guangdong Province Innovation and Strong School Project Research Program/ GR - 2023KCXTD069/Guangdong Province Innovation and Strong School Project Research Program/ GR - 2024KTSCX350/Guangdong Provincial Ordinary University Featured Innovation Project/ PT - Journal Article DEP - 20260926 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705751 OTO - NOTNLM OT - Macadamia OT - cultivated germplasm OT - gSSR markers OT - genetic diversity OT - population structure COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/26 CRDT- 2026/10/27 10:41 PHST- 2026/07/10 00:00 [received] PHST- 2026/09/09 00:00 [revised] PHST- 2026/09/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/09/26 00:00 [pmc-release] AID - life16101618 [pii] AID - life-16-01618 [pii] AID - 10.3390/life16101618 [doi] PST - epublish SO - Life (Basel). 2026 Sep 26;16(10):1618. doi: 10.3390/life16101618. PMID- 42942203 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Sep 26 TI - Surgical Management of Primary Rhegmatogenous Retinal Detachment: Experiences from a Serbian Tertiary Center. LID - 10.3390/life16101617 [doi] LID - 1617 AB - (1) Background: Rhegmatogenous retinal detachment (RRD) requires timely surgical repair, most commonly using pars plana vitrectomy (PPV), scleral buckling (SB), or pneumatic retinopexy (PR). Surgical practice varies across regions, while contemporary real-world data from Southeastern Europe remain limited. This study evaluated contemporary surgical management and 12-month outcomes of primary RRD at a Serbian tertiary referral center. (2) Methods: This retrospective, single-center observational study included 188 patients who underwent primary RRD repair between January and December 2024. Patients were treated with PPV, SB, or PR and followed for up to 12 months. Best-corrected visual acuity (BCVA) was assessed preoperatively and at postoperative day 1, day 7, and at 1, 3, 6, and 12 months. Longitudinal BCVA changes were analyzed using a linear mixed-effects model, with generalized estimating equations used as a complementary population-averaged analysis. Retinal re-detachment and postoperative intraocular pressure (IOP) elevation were also evaluated. (3) Results: PPV was performed in 104 patients (55.3%), SB in 48 (25.5%), and PR in 36 (19.1%). Baseline characteristics differed significantly among groups, including age, lens status, macular status, symptom duration, and preoperative BCVA. Longitudinal analysis demonstrated significant effects of time (F = 119.53, p < 0.001), macular status (F = 86.36, p < 0.001), symptom duration (F = 2.93, p = 0.035), and a significant time-by-surgery interaction (F = 4.86, p = 0.009). Model-estimated BCVA at 12 months was 0.77 logMAR after PPV, 0.58 after SB, and 0.28 after PR. However, PR patients had more favorable baseline characteristics. Retinal re-detachment occurred in 18.6% of patients, with no significant difference among surgical groups (p = 0.246). Early postoperative IOP elevation was significantly more frequent after PPV (22.3%) than after SB (0%) or PR (2.9%) (p < 0.001). (4) Conclusions: All three surgical approaches were associated with substantial visual recovery during 12 months of follow-up. PPV was the predominant surgical strategy, while SB remained frequently used and PR provided favorable outcomes in carefully selected patients. Because of substantial baseline differences among treatment groups, between-group outcome differences should not be interpreted as causal evidence of surgical superiority. Macular status and symptom duration were important determinants of functional recovery. FAU - Milutinovic, Vladimir AU - Milutinovic V AUID- ORCID: 0009-0001-0091-8490 AD - Clinic for Eye Diseases, University Clinical Center of Serbia, 11000 Belgrade, Serbia. FAU - Spaic, Dragan AU - Spaic D AUID- ORCID: 0000-0003-0892-3283 AD - Faculty of Medicine Foca, University of East Sarajevo, 73300 Foca, Bosnia and Herzegovina. FAU - Kostic, Jelena AU - Kostic J AD - Clinic for Eye Diseases, University Clinical Center of Serbia, 11000 Belgrade, Serbia. FAU - Risimic, Aleksandar AU - Risimic A AD - Clinic for Eye Diseases, University Clinical Center of Serbia, 11000 Belgrade, Serbia. FAU - Svetel, Marko AU - Svetel M AUID- ORCID: 0009-0009-8315-8869 AD - Clinic for Eye Diseases, University Clinical Center of Serbia, 11000 Belgrade, Serbia. FAU - Savic, Bozidar AU - Savic B AD - Institute of Veterinary Medicine of Serbia, Janisa Janulisa 14, 11000 Belgrade, Serbia. FAU - Savic, Borivoje AU - Savic B AUID- ORCID: 0000-0001-9980-2588 AD - Clinic for Eye Diseases, University Clinical Center of Serbia, 11000 Belgrade, Serbia. LA - eng PT - Journal Article DEP - 20260926 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705767 OTO - NOTNLM OT - best-corrected visual acuity OT - pars plana vitrectomy OT - pneumatic retinopexy OT - retinal re-detachment OT - rhegmatogenous retinal detachment OT - scleral buckling OT - vitreoretinal surgery COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/26 CRDT- 2026/10/27 10:41 PHST- 2026/07/26 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/09/26 00:00 [pmc-release] AID - life16101617 [pii] AID - life-16-01617 [pii] AID - 10.3390/life16101617 [doi] PST - epublish SO - Life (Basel). 2026 Sep 26;16(10):1617. doi: 10.3390/life16101617. PMID- 42942202 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Sep 26 TI - Unveiling the Immunomodulatory Potential of Medicinal Plants: A Machine Learning Framework Coupled with Experimental Validation. LID - 10.3390/life16101616 [doi] LID - 1616 AB - Medicinal plants are known for their beneficial health effects through their ability to modulate the immune system, offering promising therapeutic options for various diseases. However, traditional methods for identifying these properties can be time-consuming, labor-intensive, and expensive. This study aims to (i) use machine learning (ML) to characterize the immunomodulatory effects of medicinal plants and (ii) validate the ML model via in vitro assays to assess the predicted immunomodulatory effects. To achieve this goal, data on medicinal plants were collected from the literature, structured and encoded using pre-trained language models to generate vector representations capturing semantic relationships among botanical, chemical, and microbiological entities. Several classifiers were benchmarked through a rigorous cross-validation strategy with optimal threshold tuning, and the best-performing model (logistic regression on BiomedBERT embeddings) reached a mean cross-validated F1-score of 0.787 +/- 0.007 and, on the held-out test set (n = 37), an accuracy of 0.757, an F1-score of 0.743 and an AUC of 0.835; three of the four external validation plants were correctly classified. The results were validated experimentally using selected medicinal plants by assessing the modulation of inducible nitric oxide synthase (iNOS) and key inflammatory cytokine genes, including IL-1beta, IL-6, and TNF-alpha. C. spinosa extract up-regulated IL-1beta and TNF-alpha and down-regulated IL-6 transcripts in peritoneal macrophages, while E. fragilis extract induced a significant down-regulation of these cytokine genes and of the expression of iNOS. These preliminary results suggest that pre-trained biomedical embeddings provide a useful prioritization tool for investigating the immunomodulatory properties of medicinal plants. FAU - Assouab, Aicha AU - Assouab A AD - Biochemistry, Biotechnology and Immunophysiopathology Research Team, Health, Environment and Biotechnology Laboratory, Ain Chock Faculty of Sciences, Hassan II University of Casablanca (UH2C), Casablanca 20100, Morocco. FAU - Tajmouati, Hamza AU - Tajmouati H AD - Iktos, Artificial Intelligence for New Drug Design, 75017 Paris, France. FAU - Abdou-Allah, Fatima AU - Abdou-Allah F AD - Biochemistry, Biotechnology and Immunophysiopathology Research Team, Health, Environment and Biotechnology Laboratory, Ain Chock Faculty of Sciences, Hassan II University of Casablanca (UH2C), Casablanca 20100, Morocco. FAU - Akarid, Khadija AU - Akarid K AUID- ORCID: 0000-0003-2508-3116 AD - Biochemistry, Biotechnology and Immunophysiopathology Research Team, Health, Environment and Biotechnology Laboratory, Ain Chock Faculty of Sciences, Hassan II University of Casablanca (UH2C), Casablanca 20100, Morocco. LA - eng GR - VPMA3/Centre National pour la Recherche Scientifique et Technique (CNRST)/ PT - Journal Article DEP - 20260926 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705700 OTO - NOTNLM OT - gene expression OT - immunomodulation OT - machine learning OT - medicinal plants COIS- The authors declare that this research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/26 CRDT- 2026/10/27 10:41 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/09/26 00:00 [pmc-release] AID - life16101616 [pii] AID - life-16-01616 [pii] AID - 10.3390/life16101616 [doi] PST - epublish SO - Life (Basel). 2026 Sep 26;16(10):1616. doi: 10.3390/life16101616. PMID- 42942198 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Sep 26 TI - The Role of Transoesophageal Echocardiography Before Cardioversion of Atrial Fibrillation: Avoiding Overuse. LID - 10.3390/life16101612 [doi] LID - 1612 AB - Atrial fibrillation (AF) is the most common cardiac arrhythmia and remains a major cause of stroke, heart failure, hospitalization, and cardiovascular mortality. Electrical cardioversion is an effective strategy for restoring sinus rhythm in symptomatic patients; however, it carries a risk of thromboembolic complications when left atrial thrombi are present. Adequate oral anticoagulation substantially reduces this risk and has become a cornerstone of peri-cardioversion management. In this setting, transoesophageal echocardiography (TOE) represents the reference imaging modality for excluding left atrial appendage (LAA) thrombus and can facilitate early cardioversion in appropriately selected patients. However, the use of TOE varies considerably across institutions and patient populations, and its role has evolved from a potentially routine examination to a more targeted diagnostic strategy. Its main value lies in patients in whom adequate and uninterrupted anticoagulation cannot be reliably established, in those with additional factors associated with an increased likelihood of thromboembolism, or when early cardioversion is clinically desirable. Conversely, routine TOE before cardioversion in patients with well-documented, adequate, and uninterrupted anticoagulation provides limited additional benefit and may not be justified given the semi-invasive nature of the examination and the procedural resources required. This review examines the pathophysiological basis of thromboembolism associated with cardioversion, current guideline recommendations, the diagnostic performance of TOE, and its evolving role in the era of direct oral anticoagulants (DOACs), with particular emphasis on the appropriate selection of patients who may benefit from a TOE-guided cardioversion strategy. FAU - Cemin, Roberto AU - Cemin R AUID- ORCID: 0000-0002-5409-1281 AD - Department of Cardiology, San Maurizio Regional Hospital of Bolzano, via L. Boehler 5, 39100 Bolzano, Italy. LA - eng PT - Journal Article PT - Review DEP - 20260926 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706452 OTO - NOTNLM OT - atrial fibrillation OT - direct oral anticoagulants OT - electrical cardioversion OT - left atrial appendage OT - thromboembolism OT - transoesophageal echocardiography COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/26 CRDT- 2026/10/27 10:41 PHST- 2026/08/18 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/09/26 00:00 [pmc-release] AID - life16101612 [pii] AID - life-16-01612 [pii] AID - 10.3390/life16101612 [doi] PST - epublish SO - Life (Basel). 2026 Sep 26;16(10):1612. doi: 10.3390/life16101612. PMID- 42942197 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Sep 25 TI - Feasibility and Clinical Outcomes of a 6-Month Home-Based Telerehabilitation Program in Patients with Peripheral Arterial Disease: A Prospective Single-Arm Study. LID - 10.3390/life16101611 [doi] LID - 1611 AB - (1) Background: Peripheral arterial disease (PAD) is associated with substantial cardiovascular morbidity and functional impairment. This study evaluated the feasibility and adherence of a 6-month home-based telerehabilitation (TR) program and longitudinal changes in functional capacity, health-related quality of life (HRQoL), and selected cardiometabolic parameters. (2) Methods: This prospective, single-arm study enrolled 42 patients with PAD. Daily ambulation was monitored using a Xiaomi Smart Band 8, and participants received weekly telephone counseling. Assessments were performed at baseline, 3 months, and 6 months. (3) Results: Median daily step count increased from 5868 to 6905 steps/day (p < 0.001), whereas attainment of progressively increasing weekly targets decreased from 58.33% to 16.66% (p < 0.001). Median 6 min walk test (6MWT) distance increased from 390 m to 445 m at 6 months, a 55 m increase (p = 0.001). HRQoL, systolic blood pressure, body mass index, and total cholesterol showed significant longitudinal changes, while resting ankle-brachial index and selected cardiopulmonary exercise testing parameters remained relatively stable (p > 0.05). (4) Conclusions: The TR program was feasible and accompanied by increased activity and improved walking capacity. The divergence between absolute activity and target attainment supports multidimensional adherence assessment. Causal effects cannot be established given the single-arm design. FAU - Rotundu, Andreea AU - Rotundu A AD - Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania. AD - Clinical Rehabilitation Hospital, 700661 Iasi, Romania. FAU - Roca, Mihai AU - Roca M AD - Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania. AD - Clinical Rehabilitation Hospital, 700661 Iasi, Romania. FAU - Mastaleru, Alexandra AU - Mastaleru A AUID- ORCID: 0000-0002-0008-9696 AD - Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania. AD - Clinical Rehabilitation Hospital, 700661 Iasi, Romania. FAU - Oancea, Andra AU - Oancea A AUID- ORCID: 0000-0003-1356-9581 AD - Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania. AD - Clinical Rehabilitation Hospital, 700661 Iasi, Romania. FAU - Popescu, Gabriela AU - Popescu G AUID- ORCID: 0009-0000-7004-6846 AD - Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania. AD - Clinical Rehabilitation Hospital, 700661 Iasi, Romania. FAU - Costache, Alexandru-Dan AU - Costache AD AUID- ORCID: 0000-0001-8544-9904 AD - Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania. AD - Clinical Rehabilitation Hospital, 700661 Iasi, Romania. FAU - Chirica, Costin AU - Chirica C AUID- ORCID: 0009-0004-4813-8057 AD - Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania. FAU - Stamate, Theodor Constantin AU - Stamate TC AUID- ORCID: 0009-0001-3670-0469 AD - Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania. FAU - Grosu, Cristina AU - Grosu C AD - Clinical Rehabilitation Hospital, 700661 Iasi, Romania. AD - Department of Neurology, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. FAU - Mitu, Florin AU - Mitu F AUID- ORCID: 0000-0003-1801-9327 AD - Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania. AD - Clinical Rehabilitation Hospital, 700661 Iasi, Romania. AD - Romanian Academy of Medical Sciences, 030167 Bucharest, Romania. AD - Academy of Romanian Scientists, Ilfov 3, 050044 Bucharest, Romania. FAU - Leon, Maria Magdalena AU - Leon MM AUID- ORCID: 0000-0002-5836-1322 AD - Grigore T. Popa University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania. AD - Clinical Rehabilitation Hospital, 700661 Iasi, Romania. LA - eng GR - 351058/INNODOC-MED/ PT - Journal Article DEP - 20260925 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706065 OTO - NOTNLM OT - digital health OT - exercise adherence OT - home walking program OT - peripheral arterial disease OT - telerehabilitation OT - wearable tracking COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/25 CRDT- 2026/10/27 10:41 PHST- 2026/07/17 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - life16101611 [pii] AID - life-16-01611 [pii] AID - 10.3390/life16101611 [doi] PST - epublish SO - Life (Basel). 2026 Sep 25;16(10):1611. doi: 10.3390/life16101611. PMID- 42942194 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Sep 25 TI - Single-Lens Reconstructed Three-Dimensional Versus Two-Dimensional Visualization in Uniportal Full-Endoscopic Unilateral Laminotomy with Bilateral Decompression for Lumbar Spinal Stenosis: A Retrospective Comparative Study. LID - 10.3390/life16101608 [doi] LID - 1608 AB - BACKGROUND: Full-endoscopic unilateral laminotomy with bilateral decompression (ULBD) through a single portal lacks stereopsis, which dual-lens three-dimensional (3D) endoscopes cannot supply. This study evaluated whether a single-lens 3D reconstructed system shortens key operative steps, whether the benefit is greater with segmental deformity, and whether 12-month outcomes differ. METHODS: Sixty-seven consecutive patients undergoing single-level uniportal full-endoscopic ULBD (2023-2024) were retrospectively reviewed; the same endoscope was used in two-dimensional (2D) mode (n = 33) or single-lens 3D mode (n = 34). Allocation was not randomized; assignment to the 2D or 3D group was determined by insurance coverage rather than by the investigators. Operative times were analyzed in all 67 patients; pain and function were analyzed in the 62 who completed 12-month follow-up. Deformity was present in 14 and 17 patients (p = 0.534). Times, pain, and function over 12 months were compared by two-way ANOVA with a system x deformity interaction. RESULTS: 3D shortened time to drilling (4.4 vs. 6.1 min) and flavectomy (41.8 vs. 61.4 min; p < 0.001). The interval from initiation of drilling to completion of flavectomy-the deep portion of the procedure-was 17.9 min shorter with 3D (37.4 vs. 55.3 min; p < 0.001). The benefit depended on deformity (interaction p = 0.038): deformity prolonged that interval by 7.7 min with 2D but by 0.02 min with 3D. No complications or reoperations occurred. No statistically significant between-group difference in pain relief was detected, and the 12-month Oswestry Disability Index advantage with 3D (6.3 percentage points) fell below the minimum clinically important difference. CONCLUSIONS: Single-lens 3D reconstructed visualization shortens the deeper, working-channel portion of uniportal full-endoscopic ULBD and offsets the added time cost of deformed anatomy, but was not associated with a detectable improvement in clinical outcomes in this cohort. FAU - Chung, Yu-Hsuan AU - Chung YH AD - Doctoral Program in Translational Medicine, National Chung Hsing University, Taichung 402, Taiwan. AD - Rong Hsing Translational Medicine Research Center, National Chung Hsing University, Taichung 402, Taiwan. AD - Department of Orthopedics, Show Chwan Memorial Hospital, Changhua 500, Taiwan. AD - Department of Life Sciences, National Chung Hsing University, Taichung 402, Taiwan. FAU - Chuang, Hao-Chun AU - Chuang HC AUID- ORCID: 0000-0001-9408-0651 AD - Tainan Municipal Hospital (Managed by Show Chwan Medical Care Corporation), Tainan 701, Taiwan. AD - Department of Orthopedic Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan. FAU - Hu, Ming-Hsien AU - Hu MH AUID- ORCID: 0000-0002-2657-2352 AD - Department of Orthopedics, Show Chwan Memorial Hospital, Changhua 500, Taiwan. AD - Department of Post-Baccalaureate Medicine, College of Medicine, National Chung Hsing University, Taichung 402, Taiwan. FAU - Su, Hong-Lin AU - Su HL AD - Doctoral Program in Translational Medicine, National Chung Hsing University, Taichung 402, Taiwan. AD - Rong Hsing Translational Medicine Research Center, National Chung Hsing University, Taichung 402, Taiwan. AD - Department of Life Sciences, National Chung Hsing University, Taichung 402, Taiwan. AD - The iEGG and Animal Biotechnology Research Center, National Chung Hsing University, Taichung 402, Taiwan. LA - eng PT - Journal Article DEP - 20260925 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706338 OTO - NOTNLM OT - 3D reconstructed visualization OT - depth perception OT - interlaminar approach OT - minimally invasive spine surgery OT - neurogenic claudication OT - operative time OT - patient-reported outcome measures OT - stereopsis OT - surgical training COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/25 CRDT- 2026/10/27 10:41 PHST- 2026/08/14 00:00 [received] PHST- 2026/09/11 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - life16101608 [pii] AID - life-16-01608 [pii] AID - 10.3390/life16101608 [doi] PST - epublish SO - Life (Basel). 2026 Sep 25;16(10):1608. doi: 10.3390/life16101608. PMID- 42942171 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Sep 22 TI - Effects of Ultrasound-Guided Erector Spinae Plane Block on Early Postoperative Inflammatory and Metabolic Parameters in Patients Undergoing Breast Cancer Surgery. LID - 10.3390/life16101585 [doi] LID - 1585 AB - Background and Objectives: Surgical treatment of breast cancer is commonly associated with a pronounced inflammatory and metabolic stress response, which may adversely affect postoperative recovery. The erector spinae plane block (ESPB) is an ultrasound-guided regional anesthesia technique that has been suggested to influence perioperative stress responses through effective analgesia and reduced opioid requirements. This study aimed to evaluate the effects of ESPB on early postoperative inflammatory and metabolic parameters in patients undergoing breast cancer surgery. The present study represents a secondary exploratory analysis of a previously reported clinical cohort. The central hypothesis was that the addition of ESPB to general anesthesia (GA) would be associated with different early postoperative patterns of inflammatory and metabolic parameters compared with GA alone. Materials and Methods: This secondary exploratory analysis included 48 female patients (aged 50-65 years) scheduled for modified radical mastectomy. They were sequentially allocated in an alternating manner into two groups: one receiving only GA (n = 24) and the other GA with ESPB (n = 24). Inflammatory markers (interleukin-6, leukocyte count, neutrophil-to-lymphocyte ratio, C-reactive protein, and fibrinogen) and blood glucose levels were measured preoperatively and in the immediate postoperative period. Statistical analysis was conducted using t-tests, Mann-Whitney tests, and repeated measures ANOVA. Results: Patients who received ESPB in addition to GA had significantly lower immediate postoperative CRP levels (p < 0.001), while leukocyte and neutrophil counts showed significantly different patterns of change over time between the groups (time x group interaction: p = 0.005 and p = 0.003, respectively). While IL-6 levels did not differ significantly between the groups, immediate postoperative blood glucose levels were significantly lower in the ESPB group (p < 0.001), indicating a more favorable early postoperative glycemic profile. Conclusions: In patients undergoing breast cancer surgery, the addition of ESPB to general anesthesia was associated with different patterns of change in selected inflammatory parameters and lower blood glucose levels during the immediate postoperative period compared with general anesthesia alone. The present findings are limited to this early postoperative time point and do not establish a sustained anti-inflammatory or metabolic effect. FAU - Nikolic, Aleksandar AU - Nikolic A AD - Department of Surgery and Anesthesiology with Reanimatology, Faculty of Medicine, University of Nis, 18000 Nis, Serbia. AD - Clinic for Anesthesia and Intensive Care, University Clinical Center Nis, 18000 Nis, Serbia. FAU - Zivadinovic, Jelena AU - Zivadinovic J AUID- ORCID: 0000-0003-0230-6735 AD - Department of Surgery and Anesthesiology with Reanimatology, Faculty of Medicine, University of Nis, 18000 Nis, Serbia. AD - Clinic for Anesthesia and Intensive Care, University Clinical Center Nis, 18000 Nis, Serbia. FAU - Rankovic, Gorana Nedin AU - Rankovic GN AD - Department of Pharmacology with Toxicology, Faculty of Medicine, University of Nis, 18000 Nis, Serbia. FAU - Krtinic, Dane AU - Krtinic D AD - Department of Pharmacology with Toxicology, Faculty of Medicine, University of Nis, 18000 Nis, Serbia. AD - Clinic of Oncology, University Clinical Center Nis, 18000 Nis, Serbia. FAU - Stosic, Marija AU - Stosic M AUID- ORCID: 0000-0001-9921-8488 AD - Department of Surgery and Anesthesiology with Reanimatology, Faculty of Medicine, University of Nis, 18000 Nis, Serbia. AD - Clinic for Cardiovascular and Transplantation Surgery, University Clinical Center Nis, 18000 Nis, Serbia. FAU - Stojanovic, Milena AU - Stojanovic M AUID- ORCID: 0000-0003-1343-1937 AD - Clinic for Anesthesia and Intensive Care, University Clinical Center Nis, 18000 Nis, Serbia. FAU - Dinic, Vesna AU - Dinic V AUID- ORCID: 0000-0001-8459-4691 AD - Department of Surgery and Anesthesiology with Reanimatology, Faculty of Medicine, University of Nis, 18000 Nis, Serbia. AD - Clinic for Anesthesia and Intensive Care, University Clinical Center Nis, 18000 Nis, Serbia. FAU - Milenkovic, Nikola AU - Milenkovic N AUID- ORCID: 0000-0002-2489-5123 AD - Department of Infectious Diseases and Epidemiology, Faculty of Medicine, University of Nis, 18000 Nis, Serbia. AD - Clinic for Infectious Diseases, University Clinical Center Nis, 18000 Nis, Serbia. FAU - Pejcic, Nada AU - Pejcic N AUID- ORCID: 0009-0001-1369-6276 AD - Clinic for Anesthesia and Intensive Care, University Clinical Center Nis, 18000 Nis, Serbia. FAU - Binic, Iva AU - Binic I AUID- ORCID: 0000-0002-6457-990X AD - Department of Psychiatry, Faculty of Medicine, University of Nis, 18000 Nis, Serbia. AD - Clinic for Psychiatry, University Clinical Center Nis, 18000 Nis, Serbia. FAU - Lazarevic, Milan AU - Lazarevic M AD - Clinic for Cardiovascular and Transplantation Surgery, University Clinical Center Nis, 18000 Nis, Serbia. AD - Department of Microbiology and Immunology, Faculty of Medicine, University of Nis, 18000 Nis, Serbia. LA - eng PT - Journal Article DEP - 20260922 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706155 OTO - NOTNLM OT - breast cancer surgery OT - early postoperative inflammatory response OT - erector spinae plane block OT - metabolic parameters OT - regional anesthesia COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:41 PHST- 2026/08/13 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - life16101585 [pii] AID - life-16-01585 [pii] AID - 10.3390/life16101585 [doi] PST - epublish SO - Life (Basel). 2026 Sep 22;16(10):1585. doi: 10.3390/life16101585. PMID- 42942167 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Sep 22 TI - RNF5 Regulates HERP1 and OS9 Stability and Modulates Processing of an ERAD Substrate. LID - 10.3390/life16101581 [doi] LID - 1581 AB - Endoplasmic reticulum-associated degradation (ERAD) must dynamically adapt to cellular protein-folding demands, yet mechanisms regulating ERAD-associated adaptors remain incompletely defined. Here, we identify the E3 ubiquitin ligase RNF5 as a regulator of the ERAD adaptors HERPUD1 (HERP1) and OS9. RNF5 depletion increased HERP1 and OS9 protein abundance, while semiquantitative endpoint RT-PCR did not reveal obvious changes in their transcript signals. Available cycloheximide-chase experiments were consistent with increased adaptor persistence and more rapid loss of the ERAD-L substrate null Hong Kong alpha1-antitrypsin (NHK) after RNF5 depletion. In contrast, expression of the catalytically inactive RNF5 C42S mutant was associated with slower NHK loss, indicating that RNF5 depletion and catalytic inactivation produce distinct functional outcomes. Proteomic screening and co-immunoprecipitation supported association of RNF5 with HERP1- and OS9-containing complexes. RNF5 depletion reduced compound 225-induced HERP1 ubiquitination and adaptor loss, while wild-type RNF5 produced stronger HERP1 ubiquitination than C42S, supporting a contribution of RNF5 catalytic activity. RNF5 depletion also attenuated selected tunicamycin-induced UPR markers and 225-associated cytotoxic/apoptosis-related readouts while increasing SA-beta-galactosidase- and gammaH2AX-associated phenotypes. Together, these findings identify RNF5 as a regulator of ERAD-adaptor abundance and NHK processing, while leaving the causal relationship between adaptor regulation and the RNF5-depletion phenotype to be resolved by formal rescue and epistasis studies. FAU - Yan, Wenjing AU - Yan W AD - Department of Pharmacology and Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA. FAU - Qu, Yanyan AU - Qu Y AUID- ORCID: 0009-0005-3094-3098 AD - Division of Preclinical Innovation, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD 20850, USA. FAU - Zhong, Yongwang AU - Zhong Y AD - Department of Pharmacology and Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA. FAU - LeClair, Christopher A AU - LeClair CA AUID- ORCID: 0000-0002-2746-5938 AD - Division of Preclinical Innovation, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD 20850, USA. FAU - Rai, Ganesha AU - Rai G AD - Division of Preclinical Innovation, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD 20850, USA. FAU - Tao, Dingyin AU - Tao D AD - Division of Preclinical Innovation, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD 20850, USA. FAU - Fang, Shengyun AU - Fang S AUID- ORCID: 0000-0001-7280-5463 AD - Department of Pharmacology and Physiology, University of Maryland School of Medicine, Baltimore, MD 21201, USA. AD - Program in Oncology, UM Greenebaum Comprehensive Cancer Center, University of Maryland School of Medicine, Baltimore, MD 21201, USA. LA - eng GR - U01 AR081599/AR/NIAMS NIH HHS/United States GR - UO1AR081599-01/AR/NIAMS NIH HHS/United States PT - Journal Article DEP - 20260922 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13706468 OTO - NOTNLM OT - ER stress OT - HERPUD1 (HERP1) OT - OS9 OT - RNF5 OT - cellular senescence OT - endoplasmic reticulum-associated degradation (ERAD) OT - ubiquitination OT - unfolded protein response COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:41 PHST- 2026/08/07 00:00 [received] PHST- 2026/09/12 00:00 [revised] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - life16101581 [pii] AID - life-16-01581 [pii] AID - 10.3390/life16101581 [doi] PST - epublish SO - Life (Basel). 2026 Sep 22;16(10):1581. doi: 10.3390/life16101581. PMID- 42942166 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-1729 (Print) IS - 2075-1729 (Electronic) IS - 2075-1729 (Linking) VI - 16 IP - 10 DP - 2026 Sep 22 TI - Geographical Provenance-Associated Differences in Lipophilic Phytochemical Fingerprints and Biological Activities of Peltophorum africanum from Two South African Collection Sites. LID - 10.3390/life16101580 [doi] LID - 1580 AB - Medicinal plants collected from different environments may yield chemically and biologically distinct preparations even when they share the same botanical identity. This study compared the lipophilic phytochemical profiles and in vitro antioxidant and antibacterial activities of Peltophorum africanum leaf material collected at one purposively selected site in Bushbuckridge, Mpumalanga (MP), and one in Mafarana Village, Limpopo (LP), South Africa. Hexane and dichloromethane (DCM) extracts were examined using qualitative and quantitative phytochemical assays, thin-layer chromatography (TLC), DPPH-TLC bioautography, three antioxidant assays and antibacterial screening. The four site-derived extracts showed different phytochemical and chromatographic patterns. The MP hexane extract had the lowest DPPH IC50 (0.19 mg/mL), whereas the LP DCM extract had the lowest hydrogen peroxide-scavenging IC50 (0.48 mg/mL). Ferric reducing power IC50 values were similar across the extracts (0.48-0.55 mg/mL). In broth microdilution, only the DCM extracts inhibited Enterococcus faecalis at the tested range (MIC = 0.50 mg/mL for both sites); all other extract-organism combinations had MIC values > 1.00 mg/mL. The findings demonstrate geographical provenance-associated variation between the two sampled localities and identify assay-specific candidates for further study. They do not, however, establish that either single site represents its entire province. Broader multi-site and multi-season sampling with compound-level characterisation would strengthen geographical generalisation and mechanistic interpretation. FAU - Phasha, Mahlogonolo AU - Phasha M AUID- ORCID: 0009-0008-9373-4084 AD - Department of Biochemistry and Biotechnology, School of Science and Technology, Sefako Makgatho Health Sciences University, Pretoria 0182, South Africa. FAU - Mabasa, Vusumuzi AU - Mabasa V AUID- ORCID: 0000-0002-4423-2204 AD - Division of Epidemiology & Biostatistics, School of Public Health, Faculty of Health Sciences, University of the Witwatersrand (Wits), Johannesburg 2001, South Africa. FAU - Mothibe, Mmamosheledi E AU - Mothibe ME AUID- ORCID: 0000-0002-8613-2543 AD - Division of Pharmacology, Faculty of Pharmacy, Rhodes University, Makhanda 6139, South Africa. FAU - Nogbou, David N AU - Nogbou DN AUID- ORCID: 0000-0003-0938-0073 AD - Department of Medical Microbiology, School of Medicine, Sefako Makgatho Health Sciences University, Pretoria 0182, South Africa. FAU - Musyoki, Andrew M AU - Musyoki AM AUID- ORCID: 0000-0002-6577-6155 AD - Department of Medical Microbiology, School of Medicine, Sefako Makgatho Health Sciences University, Pretoria 0182, South Africa. FAU - Gololo, Sechene S AU - Gololo SS AUID- ORCID: 0000-0002-8059-1941 AD - Department of Biochemistry and Biotechnology, School of Science and Technology, Sefako Makgatho Health Sciences University, Pretoria 0182, South Africa. FAU - Shikwambana, Hlayiseka AU - Shikwambana H AUID- ORCID: 0000-0003-2630-6780 AD - Department of Biochemistry and Biotechnology, School of Science and Technology, Sefako Makgatho Health Sciences University, Pretoria 0182, South Africa. FAU - Mapfumari, Ntwanano S AU - Mapfumari NS AUID- ORCID: 0000-0002-5096-5367 AD - Department of Physiology, School of Medicine, Sefako Makgatho Health Sciences University, Pretoria 0182, South Africa. LA - eng PT - Journal Article DEP - 20260922 PL - Switzerland TA - Life (Basel) JT - Life (Basel, Switzerland) JID - 101580444 PMC - PMC13705773 OTO - NOTNLM OT - Peltophorum africanum OT - antibacterial activity OT - antioxidant activity OT - geographical provenance OT - lipophilic extracts OT - phytochemical fingerprint OT - thin-layer chromatography COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:41 PHST- 2026/08/09 00:00 [received] PHST- 2026/09/11 00:00 [revised] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - life16101580 [pii] AID - life-16-01580 [pii] AID - 10.3390/life16101580 [doi] PST - epublish SO - Life (Basel). 2026 Sep 22;16(10):1580. doi: 10.3390/life16101580. PMID- 42942156 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 21 TI - Cross-Disease Transcriptomic Signatures Shared by NAFLD and MI: Multicohort Machine-Learning Analysis and Exploratory IDI1 Expression Assessment. LID - 10.3390/ijms27209390 [doi] LID - 9390 AB - The molecular features shared by non-alcoholic fatty liver disease (NAFLD) and myocardial infarction (MI) remain incompletely understood. We analyzed six microarray cohorts from the GEO database to identify shared disease-associated transcriptomic features across independent NAFLD and MI populations. Weighted gene co-expression network analysis, differential expression analysis, and fuzzy c-means clustering across ordered cross-sectional NAFLD groups were used to define candidate genes. LASSO regression, random forest, and SVM-RFE identified consensus computational candidates, which were examined using exploratory classification, receiver operating characteristic curves, calibration curves, decision curve analysis, and SHapley Additive exPlanations. IDI1 was selected for exploratory expression assessment by qPCR in patients with established MI and in a mouse model of acute ischemic myocardial injury. Fifty-three shared candidate genes were identified, and five genes were retained by all three feature-selection methods. Single-gene ROC analyses showed SULF2, GADD45A, LIN7A, and IDI1 within the analyzed case-control datasets. IDI1 expression was higher in patients with MI than in healthy controls (p = 0.004), as well as in mice blood (p = 0.034) and infarcted myocardium (p = 0.0072) following LAD ligation. These findings indicate shared transcriptomic links between NAFLD and MI, supporting an association between IDI1 and myocardial injury. FAU - Qiao, Jilei AU - Qiao J AD - Department of Cardiology, First Affiliated Hospital of Anhui Medical University, Hefei 230032, China. FAU - Chen, Shuran AU - Chen S AD - Department of General Surgery, First Affiliated Hospital of Anhui Medical University, Hefei 230032, China. FAU - Li, Shuo AU - Li S AD - Department of Cardiology, First Affiliated Hospital of Anhui Medical University, Hefei 230032, China. FAU - Shao, Wenbo AU - Shao W AD - Department of Cardiology, First Affiliated Hospital of Anhui Medical University, Hefei 230032, China. FAU - Zhang, Yiran AU - Zhang Y AD - The First Clinical College, Anhui Medical University, Hefei 230022, China. FAU - Huang, Ying AU - Huang Y AD - Department of Cardiology, First Affiliated Hospital of Anhui Medical University, Hefei 230032, China. FAU - Xie, Yangjing AU - Xie Y AUID- ORCID: 0000-0001-9568-6110 AD - Department of Cardiology, First Affiliated Hospital of Anhui Medical University, Hefei 230032, China. LA - eng GR - 2022xkj054/Scientific Research Fund of Anhui Medical University/ GR - 202427b10020146/Clinical and Translational Research Project of Anhui Province/ GR - 2023zhyx-B06/Anhui Institute of Translational Medicine/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - *Non-alcoholic Fatty Liver Disease/genetics/metabolism MH - Humans MH - *Myocardial Infarction/genetics/metabolism MH - Animals MH - Mice MH - *Transcriptome/genetics MH - *Machine Learning MH - Gene Expression Profiling MH - Male MH - Female MH - ROC Curve MH - Case-Control Studies MH - Disease Models, Animal PMC - PMC13704942 OTO - NOTNLM OT - IDI1 OT - liver-heart axis OT - machine learning OT - myocardial infarction OT - non-alcoholic fatty liver disease COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/21 CRDT- 2026/10/27 10:41 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ijms27209390 [pii] AID - ijms-27-09390 [pii] AID - 10.3390/ijms27209390 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 21;27(20):9390. doi: 10.3390/ijms27209390. PMID- 42942150 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 21 TI - Immune Response Gene Polymorphisms and Cervical Cancer Susceptibility. LID - 10.3390/ijms27209384 [doi] LID - 9384 AB - Cervical cancer (CxCa) remains one of the most common malignancies in women worldwide. Although almost all cases are associated with chronic infection with oncogenic human papillomavirus (HPV) types, not every infected woman develops the disease, indicating a significant role of genetic factors. This study reviewed 131 studies on genes related to immune system function and their impact on the risk of developing cervical cancer. The majority of the studies analyzed were conducted in Asian populations, primarily Chinese and Indian. The analysis focused in particular on polymorphisms in genes involved in the antiviral response, inflammatory processes, antigen presentation, and regulation of lymphocyte activity. Most of the evidence points to the importance of human leukocyte antigens (HLA) genes, Toll-like receptors (TLRs), interleukins (ILs), tumor necrosis factor alpha (TNF-alpha), and interferons (IFNs). Variants in these genes may influence the effectiveness of HPV clearance, susceptibility to persistent infection, and the risk of progression of precancerous lesions to invasive cancer. It is worth emphasizing that identifying genetic risk factors may, in the future, support the development of personalized prevention strategies, screening tests, and the assessment of the individual risk of disease. Nevertheless, most available findings warrant further validation in large and diverse populations. In summary, cervical cancer is the result of a complex interaction between HPV infection and the host's genetic predisposition. Research on immune response genes provides valuable information about the mechanisms of disease development, and may contribute to improved methods of prevention and early detection. FAU - Dwornik, Roksana AU - Dwornik R AD - Department of Genetics and Pathology, International Hereditary Cancer Center, Pomeranian Medical University, 71-252 Szczecin, Poland. FAU - Cylwik, Dagmara AU - Cylwik D AD - Department of Genetics and Pathology, International Hereditary Cancer Center, Pomeranian Medical University, 71-252 Szczecin, Poland. FAU - Bialkowska, Katarzyna AU - Bialkowska K AD - Department of Genetics and Pathology, International Hereditary Cancer Center, Pomeranian Medical University, 71-252 Szczecin, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (HLA Antigens) SB - IM MH - Humans MH - Female MH - *Uterine Cervical Neoplasms/genetics/immunology/virology MH - *Genetic Predisposition to Disease MH - *Polymorphism, Genetic MH - Human Papillomavirus Viruses/immunology MH - Papillomavirus Infections/genetics/immunology/complications MH - HLA Antigens/genetics PMC - PMC13705446 OTO - NOTNLM OT - Cervical cancer OT - Human papillomavirus (HPV) OT - Immune response gene polymorphisms COIS- The authors declare no competing interests. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/21 CRDT- 2026/10/27 10:41 PHST- 2026/08/25 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ijms27209384 [pii] AID - ijms-27-09384 [pii] AID - 10.3390/ijms27209384 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 21;27(20):9384. doi: 10.3390/ijms27209384. PMID- 42942148 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 21 TI - Derivatives of Dictyostelium Differentiation-Inducing Factor-3 Promote Autophagic Flux, Mitochondrial Dysfunction and Iron-Associated Oxidative Stress. LID - 10.3390/ijms27209380 [doi] LID - 9380 AB - Novel anticancer agents are needed to overcome therapeutic resistance, and autophagy represents a promising target. The chlorinated alkylphenones Dictyostelium differentiation-inducing factors 1 and 3 (DIF-1 and DIF-3) and their derivatives exert anti-proliferative and anti-metastatic effects; however, their molecular mechanisms remain incompletely understood. Using the GFP-LC3-RFP-LC3DeltaG autophagic flux reporter, we identified Bu-DIF-3 and CP-DIF-3 as autophagy-promoting derivatives and characterized their actions principally in HeLa cells, with selected autophagy and AMP-activated protein kinase (AMPK) measurements in HAP1 cells. Both suppressed mTORC1 signaling, activated AMPK, and reduced mitochondrial membrane potential (MMP). Bu-DIF-3 additionally induced reactive oxygen species and redox stress response genes, including SLC7A11 and CHAC1. SLC7A11 induction may reflect a compensatory antioxidant response. Unlike CP-DIF-3, Bu-DIF-3 increased the FerroOrange-detectable labile Fe(2+) signal, and deferoxamine partially restored the reduced MTT signal, supporting possible iron involvement in reduced metabolic activity. Thus, modification of the DIF-3 methoxy group modulates autophagy-promoting activity and associated stress responses. Bu-DIF-3 was associated with oxidative stress and altered labile Fe(2+) homeostasis; whether a ferroptosis-related mechanism contributes remains unresolved. These findings provide a cellular basis for further evaluation of Bu-DIF-3 and related DIF derivatives. FAU - Cai, Yue AU - Cai Y AD - Laboratory of Food and Life Science, Institute of Human Sciences, Waseda University, Saitama 359-1192, Japan. FAU - Yamada, Ryu AU - Yamada R AD - Laboratory of Food and Life Science, Institute of Human Sciences, Waseda University, Saitama 359-1192, Japan. FAU - Marugame, Yuki AU - Marugame Y AD - Laboratory of Food and Life Science, Institute of Human Sciences, Waseda University, Saitama 359-1192, Japan. FAU - Yano, Satoshi AU - Yano S AD - Laboratory of Food and Life Science, Institute of Human Sciences, Waseda University, Saitama 359-1192, Japan. FAU - Okumura, Fumihiko AU - Okumura F AD - Department of Food and Health Sciences, International College of Arts and Sciences, Fukuoka Women's University, Fukuoka 813-8529, Japan. FAU - Kikuchi, Haruhisa AU - Kikuchi H AUID- ORCID: 0000-0001-6938-0185 AD - Division of Natural Medicines, Faculty of Pharmacy, Keio University, Tokyo 105-8512, Japan. FAU - Kubohara, Yuzuru AU - Kubohara Y AUID- ORCID: 0000-0001-9231-9833 AD - Faculty of Health and Sports Science, Juntendo University, Chiba 270-1695, Japan. FAU - Hara, Taichi AU - Hara T AUID- ORCID: 0000-0002-4722-6336 AD - Laboratory of Food and Life Science, Institute of Human Sciences, Waseda University, Saitama 359-1192, Japan. LA - eng GR - 24K23788 [YM]; 22K17816 and 24K20683 [SY]; 24K08703 [YK]; and 20H03408 [TH]/Japan Society for the Promotion of Science/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - E1UOL152H7 (Iron) RN - 0 (Hexanones) RN - 0 (Reactive Oxygen Species) RN - 111050-72-7 (1-((3,5-dichloro)-2,6-dihydroxy-4-methoxyphenyl)-1-hexanone) RN - EC 2.7.11.31 (AMP-Activated Protein Kinases) RN - EC 2.7.11.1 (Mechanistic Target of Rapamycin Complex 1) SB - IM MH - *Oxidative Stress/drug effects MH - Humans MH - *Autophagy/drug effects MH - *Iron/metabolism MH - *Hexanones/pharmacology/chemistry MH - *Mitochondria/drug effects/metabolism MH - *Dictyostelium/metabolism MH - Reactive Oxygen Species/metabolism MH - HeLa Cells MH - Membrane Potential, Mitochondrial/drug effects MH - Signal Transduction/drug effects MH - AMP-Activated Protein Kinases/metabolism MH - Mechanistic Target of Rapamycin Complex 1/metabolism PMC - PMC13705388 OTO - NOTNLM OT - Dictyostelium discoideum OT - autophagy OT - differentiation-inducing factor OT - iron-associated oxidative stress OT - mTORC1 OT - reactive oxygen species COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/21 CRDT- 2026/10/27 10:41 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ijms27209380 [pii] AID - ijms-27-09380 [pii] AID - 10.3390/ijms27209380 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 21;27(20):9380. doi: 10.3390/ijms27209380. PMID- 42942146 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 21 TI - Beyond Homologous Recombination: Functional Modules of Olaparib Resistance Revealed by CRISPR Screens. LID - 10.3390/ijms27209381 [doi] LID - 9381 AB - Clinical use of PARP inhibitors (PARPi), including olaparib, has improved outcomes for patients with BRCA1/2-mutant and homologous recombination (HR)-deficient tumors. However, acquired resistance remains a major limitation to their efficacy. CRISPR-Cas9 screening approaches have been developed to identify genetic drivers of PARPi resistance. However, differences in experimental conditions, library coverage, and selection design complicate comparisons across studies. In this review, we present a harmonized reanalysis of CRISPR knockout olaparib resistance screens to identify recurrent resistance-associated genes and pathways. Datasets with raw data were collected from the BioGRID ORCS database. Resistance hits identified in at least two screens were subjected to Reactome pathway-enrichment analysis and organized into functional modules. Using this approach, we identified eight modules: DNA-damage repair and replication stress; cell-cycle regulation; chromatin remodeling and histone modification; post-translational regulation; mitochondrial function; TGF-beta and mTOR signaling; RNA processing and nucleocytoplasmic transport; and emerging adaptive mechanisms. Importantly, mitochondrial function genes were observed as recurrent hits, but their relation to olaparib resistance is underinvestigated. Our integrative analysis suggests that PARPi resistance is a complex, adaptive phenotype shaped by recurrent but context-dependent vulnerabilities across multiple cellular programs. This framework prioritizes candidate resistance drivers for validation and highlights directions for the design of combination therapies aimed at overcoming PARPi resistance. FAU - Dildabayeva, Dana AU - Dildabayeva D AUID- ORCID: 0000-0003-2109-132X AD - Department of Biological and Biomedical Sciences, School of Medicine, Nazarbayev University, Astana 010000, Kazakhstan. FAU - Samarkhanova, Diana AU - Samarkhanova D AUID- ORCID: 0000-0002-6558-6546 AD - Department of Biological and Biomedical Sciences, School of Medicine, Nazarbayev University, Astana 010000, Kazakhstan. FAU - Lezina, Larissa AU - Lezina L AUID- ORCID: 0009-0002-8580-1327 AD - Department of Biological and Biomedical Sciences, School of Medicine, Nazarbayev University, Astana 010000, Kazakhstan. LA - eng GR - AP234846678/Science Committee of the Ministry of Science and Higher Education of the Republic of Kazakhstan/ PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - WOH1JD9AR8 (olaparib) RN - 0 (Phthalazines) RN - 0 (Piperazines) RN - 0 (Poly(ADP-ribose) Polymerase Inhibitors) SB - IM MH - *Phthalazines/pharmacology/therapeutic use MH - *Piperazines/pharmacology/therapeutic use MH - Humans MH - *Drug Resistance, Neoplasm/genetics MH - *Homologous Recombination MH - *Poly(ADP-ribose) Polymerase Inhibitors/pharmacology/therapeutic use MH - *CRISPR-Cas Systems MH - Animals MH - DNA Repair PMC - PMC13705357 OTO - NOTNLM OT - CRISPR screen OT - PARP inhibitor resistance OT - functional genomics OT - olaparib COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/21 CRDT- 2026/10/27 10:41 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ijms27209381 [pii] AID - ijms-27-09381 [pii] AID - 10.3390/ijms27209381 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 21;27(20):9381. doi: 10.3390/ijms27209381. PMID- 42942143 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 21 TI - Bioactive and Molecular Characterization of Mealworm (Tenebrio molitor) Powder: Nutritional and Functional Implications for Crackers. LID - 10.3390/ijms27209378 [doi] LID - 9378 AB - Edible insects are increasingly recognized as a viable alternative food source, highlighting the urgent need for systematic research into their integration into conventional food products. This study examined the effect of adding mealworm powder (Tenebrio molitor) to crackers, focusing on factors such as color, texture, nutritional value, antioxidant properties, starch digestibility, and molecular structure. As the level of mealworm powder increased (from 2% to 10%), the crackers became darker, while instrumental hardness and fracture time decreased, indicating that a more brittle structure formed. Nutritionally, the enriched crackers contained higher protein, fat, and mineral content, while their carbohydrate content decreased; moreover, their protein profile was modified. Antioxidant activity and total phenolic content also rose in proportion to the level of mealworm enrichment. FTIR analysis revealed differences in spectral regions associated with proteins, lipids, and carbohydrates, indicating compositional and local molecular-environment changes following mealworm powder incorporation. Mealworm powder incorporation markedly decreased rapidly digestible starch and increased slowly digestible starch, whereas the predicted glycemic index showed only a modest but statistically significant reduction at higher supplementation levels. Furthermore, sensory evaluation indicated that consumer acceptance was optimal at a low inclusion level of 2%. Overall, mealworm powder represents a promising ingredient for improving selected nutritional and functional characteristics of crackers; however, its inclusion level should be optimized to maintain sensory quality. FAU - Zielinska, Ewelina AU - Zielinska E AUID- ORCID: 0000-0002-4921-294X AD - Department of Analysis and Evaluation of Food Quality, University of Life Sciences in Lublin, Skromna Str. 8, 20-704 Lublin, Poland. FAU - Jakubczyk, Anna AU - Jakubczyk A AD - Department of Biochemistry and Food Chemistry, University of Life Sciences in Lublin, Skromna Str. 8, 20-704 Lublin, Poland. FAU - Jaglinska, Kamila AU - Jaglinska K AD - Department of Physical Chemistry, Medical University of Lublin, Chodzki 4a, 20-093 Lublin, Poland. FAU - Polak, Beata AU - Polak B AUID- ORCID: 0000-0003-4237-648X AD - Department of Physical Chemistry, Medical University of Lublin, Chodzki 4a, 20-093 Lublin, Poland. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Powders) RN - 0 (Antioxidants) RN - 0 (Phenols) RN - 9005-25-8 (Starch) SB - IM MH - Animals MH - *Tenebrio/chemistry MH - *Nutritive Value MH - Powders/chemistry MH - Antioxidants/analysis/chemistry MH - Edible Insects/chemistry MH - Phenols/analysis MH - Starch/chemistry PMC - PMC13705389 OTO - NOTNLM OT - FTIR analysis OT - consumer acceptance OT - edible insects OT - entomophagy OT - phenolic compounds OT - predicted glycemic index OT - starch digestibility OT - texture COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/21 CRDT- 2026/10/27 10:41 PHST- 2026/08/20 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ijms27209378 [pii] AID - ijms-27-09378 [pii] AID - 10.3390/ijms27209378 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 21;27(20):9378. doi: 10.3390/ijms27209378. PMID- 42942142 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 21 TI - Bremachlorin-Based Photodynamic-Therapy-Induced Effects in Low-Oxygenated Pancreatic Ductal Adenocarcinoma (PDAC) Tumor Microenvironment. LID - 10.3390/ijms27209374 [doi] LID - 9374 AB - Photodynamic therapy (PDT) is a minimally invasive treatment whose efficacy is strongly influenced by tumor oxygenation and the tumor microenvironment (TME). Pancreatic ductal adenocarcinoma (PDAC) is characterized by severe hypoxia, dense stroma, and immune suppression, limiting therapeutic responsiveness. In this study, we investigated how Bremachlorin-based PDT remodels the TME in two murine PDAC models with distinct immune phenotypes (T-cell-high and T-cell-low) and compared them with the immunogenic MC38 colon carcinoma model. Single-fiber reflectance and fluorescence spectroscopy were used to monitor tumor physiology and photosensitizer dynamics during PDT, while multiplex immunohistochemistry enabled spatial profiling of stromal and immune components post-treatment. PDAC tumors exhibited significantly lower baseline oxygenation than MC38 tumors, which was not substantially improved by reducing the PDT fluence rate from 116 to 50 mW.cm(-2). Nevertheless, PDT reduced collagen type I content in both PDAC models, indicating stromal remodeling. Importantly, illumination with a moderate fluence rate of 50 mW.cm(-2) significantly increased CD8(+) T-cell infiltration and improved the CD8(+)/neutrophil ratio in T-cell-high PDAC tumors, suggesting a shift toward a less immunosuppressive immune landscape. These findings demonstrate that Bremachlorin-PDT can modulate the PDAC tumor microenvironment and highlight the importance of immune context in shaping PDT outcomes, supporting rational optimization of PDT-based combination therapies. FAU - McMorrow, Roisin AU - McMorrow R AD - Department of Radiology and Nuclear Medicine, Erasmus MC Cancer Institute, University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands. AD - Department of Molecular Genetics, Erasmus MC Cancer Institute, University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands. FAU - de Bruijn, Henriette S AU - de Bruijn HS AUID- ORCID: 0000-0003-3122-8385 AD - Department of Otorhinolaryngology and Head and Neck Surgery, Erasmus MC Cancer Institute, University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands. FAU - Que, Ivo AU - Que I AD - Department of Radiology and Nuclear Medicine, Erasmus MC Cancer Institute, University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands. AD - Department of Molecular Genetics, Erasmus MC Cancer Institute, University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands. FAU - Lila, Karishma AU - Lila K AD - Department of Pathology, Erasmus MC Cancer Institute, University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands. FAU - van den Bosch, Thierry P P AU - van den Bosch TPP AUID- ORCID: 0000-0002-7223-4565 AD - Department of Pathology, Erasmus MC Cancer Institute, University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands. FAU - Doukas, Michail AU - Doukas M AUID- ORCID: 0000-0002-8611-840X AD - Department of Pathology, Erasmus MC Cancer Institute, University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands. FAU - Lowik, Clemens W G M AU - Lowik CWGM AD - Department of Radiology and Nuclear Medicine, Erasmus MC Cancer Institute, University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands. FAU - Mezzanotte, Laura AU - Mezzanotte L AUID- ORCID: 0000-0001-5592-8904 AD - Department of Radiology and Nuclear Medicine, Erasmus MC Cancer Institute, University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands. AD - Department of Molecular Genetics, Erasmus MC Cancer Institute, University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands. FAU - Robinson, Dominic J AU - Robinson DJ AD - Department of Otorhinolaryngology and Head and Neck Surgery, Erasmus MC Cancer Institute, University Medical Center Rotterdam, 3015 GD Rotterdam, The Netherlands. LA - eng GR - The Erasmus Foundation/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (bremachlorin) RN - 0 (Photosensitizing Agents) RN - 0 (Chlorophyllides) RN - S88TT14065 (Oxygen) RN - 0 (Drug Combinations) RN - 0 (Porphyrins) SB - IM MH - *Tumor Microenvironment/drug effects MH - *Carcinoma, Pancreatic Ductal/drug therapy/pathology/metabolism/immunology MH - *Photochemotherapy/methods MH - Animals MH - Mice MH - *Pancreatic Neoplasms/drug therapy/pathology/metabolism MH - Humans MH - *Photosensitizing Agents/pharmacology MH - Cell Line, Tumor MH - Chlorophyllides MH - *Oxygen/metabolism MH - Drug Combinations MH - Porphyrins PMC - PMC13705006 OTO - NOTNLM OT - Bremachlorin OT - colon adenocarcinoma OT - fluence rate OT - pancreatic ductal adenocarcinoma OT - photodynamic therapy OT - spectroscopy OT - tumor microenvironment COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/21 CRDT- 2026/10/27 10:41 PHST- 2026/07/15 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ijms27209374 [pii] AID - ijms-27-09374 [pii] AID - 10.3390/ijms27209374 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 21;27(20):9374. doi: 10.3390/ijms27209374. PMID- 42942139 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 21 TI - Chronic Intermittent Hypoxia and MASLD in Obstructive Sleep Apnea: A Critical Appraisal of Mechanistic and Causal Evidence. LID - 10.3390/ijms27209372 [doi] LID - 9372 AB - Obstructive sleep apnea (OSA) and metabolic dysfunction-associated steatotic liver disease (MASLD) frequently coexist and share a common cardiometabolic substrate through a shared cardiometabolic substrate. This narrative review critically appraises the epidemiological, experimental, and interventional evidence proposed to link chronic intermittent hypoxia (CIH) and sleep fragmentation to hepatic steatosis, steatohepatitis, and fibrosis. We summarize the candidate mechanisms, including mitochondrial oxidative stress, hypoxia-inducible factor 1-alpha signaling, sympathetically driven lipolysis, and Kupffer and stellate cell activation and then examine the limits of the evidence supporting them: hypoxia-induced hypophagia and the scarcity of pair-fed controls in rodent models, species differences in the physiological response to hypoxia, publication bias, common-cause confounding by age and systemic inflammation, and the rarity of progressive liver disease in lean patients with anatomically driven OSA. We further review non-invasive diagnostic tools, polysomnographic hypoxemia indices, transient elastography, METS-IR, FIB-4, and emerging biomarkers, such as omentin-1 and follistatin-like 1, that enable bidirectional screening between sleep and hepatology clinics. Finally, we discuss therapeutic strategies, including continuous positive airway pressure, structured weight loss, and incretin-based pharmacotherapy (GLP-1 and dual GIP/GLP-1 receptor agonists), highlighting recent phase 3 evidence for tirzepatide in OSA and semaglutide in metabolic dysfunction-associated steatohepatitis. Current evidence supports CIH as a context-dependent amplifier of liver injury in patients with pre-existing metabolic dysfunction rather than as an established independent cause of MASH. This interpretation still justifies integrated screening and treatment pathways, while identifying the study designs needed to test causality directly. FAU - Cebuc Simeanu, Georgiana-Mihaela AU - Cebuc Simeanu GM AUID- ORCID: 0000-0002-9618-9500 AD - Doctoral School, University of Medicine and Pharmacy, 200349 Craiova, Romania. FAU - Dragne, Ioana-Gabriela AU - Dragne IG AUID- ORCID: 0009-0004-8957-690X AD - Doctoral School, University of Medicine and Pharmacy, 200349 Craiova, Romania. FAU - Mitrea, Adina AU - Mitrea A AD - Department of Diabetes, Nutrition and Metabolic Diseases, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Clenciu, Diana AU - Clenciu D AD - Department of Diabetes, Nutrition and Metabolic Diseases, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Protasiewicz-Timofticiuc, Diana-Cristina AU - Protasiewicz-Timofticiuc DC AD - Department of Diabetes, Nutrition and Metabolic Diseases, Faculty of Midwives and Nursing, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Popa, Alina-Liliana AU - Popa AL AD - Doctoral School, University of Medicine and Pharmacy, 200349 Craiova, Romania. FAU - Mitroi, Denisa-Maria AU - Mitroi DM AUID- ORCID: 0009-0006-5130-6757 AD - Doctoral School, University of Medicine and Pharmacy, 200349 Craiova, Romania. FAU - Pirvu, Bianca-Florentina AU - Pirvu BF AUID- ORCID: 0009-0001-1431-9915 AD - Doctoral School, University of Medicine and Pharmacy, 200349 Craiova, Romania. FAU - Efrem, Ion-Cristian AU - Efrem IC AUID- ORCID: 0000-0001-8563-347X AD - Department of Internal Medicine-Internal Medicine, Faculty of Dentistry, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Glodeanu, Adina Dorina AU - Glodeanu AD AD - Department of Internal Medicine-Internal Medicine, Faculty of Dentistry, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Mirea, Adina-Andreea AU - Mirea AA AUID- ORCID: 0009-0001-4039-0811 AD - Department of Oro-Dental Prevention and Oral Health, Faculty of Dentistry, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. AD - Research Centre in Dental Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Stefan, Adela-Gabriela AU - Stefan AG AUID- ORCID: 0000-0003-1159-3617 AD - Department of Diabetes, Nutrition and Metabolic Diseases, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Rosu, Maria-Magdalena AU - Rosu MM AD - Department of Diabetes, Nutrition and Metabolic Diseases, Faculty of Midwives and Nursing, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Radu-Gheonea, Theodora-Claudia AU - Radu-Gheonea TC AD - Department of Diabetes, Nutrition and Metabolic Diseases, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Vladu, Beatrice-Elena AU - Vladu BE AD - Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Efrem, Ana-Maria AU - Efrem AM AD - Faculty of Medicine, University of Medicine and Pharmacy "Iuliu Hatieganu" Cluj-Napoca, 400012 Cluj-Napoca, Romania. FAU - Dijmarescu, Lorena-Anda AU - Dijmarescu LA AD - Department of Obstetrics-Gynecology, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Fortofoiu, Mircea-Catalin AU - Fortofoiu MC AUID- ORCID: 0000-0002-3341-1442 AD - Doctoral School, University of Medicine and Pharmacy, 200349 Craiova, Romania. AD - Department of Medical Semiology, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - Humans MH - *Sleep Apnea, Obstructive/complications/metabolism/therapy MH - Animals MH - *Hypoxia/metabolism/complications MH - *Fatty Liver/metabolism/etiology PMC - PMC13705259 OTO - NOTNLM OT - CPAP OT - GLP-1 receptor agonists OT - causal inference OT - chronic intermittent hypoxia OT - hepatic fibrosis OT - insulin resistance OT - metabolic dysfunction-associated steatotic liver disease OT - obstructive sleep apnea OT - sleep fragmentation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/21 CRDT- 2026/10/27 10:41 PHST- 2026/09/30 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ijms27209372 [pii] AID - ijms-27-09372 [pii] AID - 10.3390/ijms27209372 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 21;27(20):9372. doi: 10.3390/ijms27209372. PMID- 42942128 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 21 TI - Whole-Exome Sequencing in Patients with Tooth Agenesis and Neurodevelopmental Disorders: A Descriptive Case Series. LID - 10.3390/ijms27209357 [doi] LID - 9357 AB - Tooth agenesis (TA) is a common developmental abnormality that may occur as an isolated trait or as part of a multisystem disorder. Its genetic architecture remains incompletely characterized, particularly in individuals with neurodevelopmental disorders (NDDs). This descriptive case series aimed to investigate potentially relevant genetic variants in a clinically selected cohort of patients with TA using whole-exome sequencing (WES). Ten patients with TA and neurological or neurodevelopmental conditions were recruited at the IRCCS Oasi Research Institute of Troina between January 2024 and July 2026. The cohort included eight males and two females (age range: 5-50 years). The associated clinical spectrum included autism spectrum disorder (ASD), intellectual disability (ID), language impairment, behavioral abnormalities, and Down syndrome (DS). WES was performed in the examined cohort and variants were prioritized according to population frequency, predicted functional impact, inheritance pattern, and phenotypic concordance. The cohort comprised nine patients with hypodontia and one with oligodontia. Thirty variants involving 28 genes were identified. A homozygous GLI3 p.Gly727Arg variant, inherited from two heterozygous parents, was detected in an eight-year-old boy with ASD, global developmental delay, and hypodontia. Pathogenic or likely pathogenic loss-of-function variants in BCL11B were identified in two girls with ID and hypodontia. A likely pathogenic NIPBL splice-site variant was identified in a 15-year-old boy with hypodontia. His similarly affected father carried a novel FGFR1 p.Asn185His variant that was absent in the son, excluding it as the sole explanation for the familial dental phenotype. A 50-year-old man with DS and oligodontia carried the recurrent pathogenic BBS1 p.Met390Arg allele in the heterozygous state, together with variants of uncertain significance in PTH1R and STAT3. However, none of these variants independently explained his dental phenotype. The remaining variants were primarily relevant to the associated neurological phenotypes or represented secondary findings. This descriptive case series illustrates the clinical and genetic heterogeneity of TA in individuals with NDDs. Although the findings do not establish causal relationships with TA, they highlight the importance of distinguishing the general clinical significance of a variant from its specific relevance to dental development. Replication in larger cohorts and functional validation will be required to clarify the contribution of the identified candidate variants. FAU - Cali, Francesco AU - Cali F AUID- ORCID: 0000-0001-8667-8223 AD - Oasi Research Institute-IRCCS, 94018 Troina, Italy. AD - Department Medicine and Surgery, Kore University of Enna, 94100 Enna, Italy. FAU - Musumeci, Antonino AU - Musumeci A AUID- ORCID: 0000-0002-8999-3365 AD - Oasi Research Institute-IRCCS, 94018 Troina, Italy. FAU - Vinci, Mirella AU - Vinci M AUID- ORCID: 0000-0003-3026-8510 AD - Oasi Research Institute-IRCCS, 94018 Troina, Italy. FAU - L'Episcopo, Simona AU - L'Episcopo S AUID- ORCID: 0009-0001-9399-6104 AD - Oasi Research Institute-IRCCS, 94018 Troina, Italy. FAU - Bartolone, Massimiliano AU - Bartolone M AUID- ORCID: 0009-0009-6715-8869 AD - Oasi Research Institute-IRCCS, 94018 Troina, Italy. FAU - Gloria, Angelo AU - Gloria A AUID- ORCID: 0000-0002-2848-2844 AD - Oasi Research Institute-IRCCS, 94018 Troina, Italy. FAU - Ferri, Raffaele AU - Ferri R AUID- ORCID: 0000-0001-6937-3065 AD - Oasi Research Institute-IRCCS, 94018 Troina, Italy. FAU - Treccarichi, Simone AU - Treccarichi S AUID- ORCID: 0000-0001-5631-9991 AD - Oasi Research Institute-IRCCS, 94018 Troina, Italy. FAU - Fallea, Antonio AU - Fallea A AUID- ORCID: 0000-0002-1927-9465 AD - Oasi Research Institute-IRCCS, 94018 Troina, Italy. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - Humans MH - Male MH - Female MH - *Exome Sequencing/methods MH - Child MH - Adolescent MH - *Anodontia/genetics MH - *Neurodevelopmental Disorders/genetics MH - Child, Preschool MH - Adult MH - Middle Aged MH - Young Adult MH - Phenotype PMC - PMC13704949 OTO - NOTNLM OT - dental agenesis OT - neurodevelopmental disorders OT - next generation sequencing OT - tooth agenesis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/21 CRDT- 2026/10/27 10:41 PHST- 2026/09/22 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ijms27209357 [pii] AID - ijms-27-09357 [pii] AID - 10.3390/ijms27209357 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 21;27(20):9357. doi: 10.3390/ijms27209357. PMID- 42942126 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 21 TI - Heparinoid Cream Restores Epidermal Barrier Homeostasis and Attenuates Type 2 Inflammation in MC903-Induced Dermatitis. LID - 10.3390/ijms27209360 [doi] LID - 9360 AB - Epidermal barrier dysfunction and cutaneous inflammation are closely interconnected in inflammatory skin diseases. However, the molecular effects of heparinoid cream on inflamed skin remain incompletely understood. In this study, we investigated the effects of heparinoid cream on epidermal barrier homeostasis and cutaneous inflammation using an MC903-induced dermatitis model. Eight-week-old female BALB/c mice were treated topically with MC903 for 7 consecutive days, with or without concomitant application of heparinoid cream. Skin barrier function, histological changes, inflammatory markers, and gene expression were evaluated by transepidermal water loss measurements, histology, immunohistochemistry, quantitative PCR, and RNA sequencing. Therapeutic effects were also assessed by applying heparinoid cream after dermatitis had been established. RNA sequencing identified 816 differentially expressed genes following heparinoid cream treatment, including 146 upregulated and 670 downregulated genes. Upregulated genes were enriched in epidermal differentiation- and barrier-related pathways, whereas downregulated genes were enriched in immune and inflammatory pathways. Heparinoid cream significantly reduced transepidermal water loss and epidermal thickness, suppressed Il13 and Tslp expression, and reduced epidermal KLK5 and TSLP expression. In parallel, expression of multiple epidermal differentiation- and barrier-associated genes, including members of the Lce family, Flg, and Flg2, was restored. Therapeutic application of heparinoid cream after establishment of dermatitis was also associated with improved barrier function, increased Flg expression, reduced Tslp mRNA expression, and decreased epidermal KLK5 and TSLP immunohistochemical staining. Secondary reanalysis of a previously published human keratinocyte RNA-seq dataset revealed changes in selected barrier- and innate immunity-associated genes following MPS treatment under non-inflammatory monolayer culture conditions. These findings demonstrate that heparinoid cream treatment is associated with concurrent improvements in epidermal barrier-related parameters and attenuation of type 2 inflammatory responses in MC903-induced dermatitis. These findings provide molecular insights into the changes associated with topical application of a heparinoid-containing formulation in inflamed skin, although the specific contribution of MPS remains to be established. FAU - Yasuda, Ayaka AU - Yasuda A AD - Department of Dermatology, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Fukuoka, Japan. FAU - Saito-Sasaki, Natsuko AU - Saito-Sasaki N AD - Department of Dermatology, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Fukuoka, Japan. FAU - Hara, Reiko AU - Hara R AD - Department of Dermatology, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Fukuoka, Japan. FAU - Sawada, Yu AU - Sawada Y AUID- ORCID: 0000-0001-8793-708X AD - Department of Dermatology, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Fukuoka, Japan. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Heparinoids) RN - 0 (Filaggrin Proteins) RN - 0 (Cytokines) SB - IM MH - Animals MH - Female MH - *Epidermis/drug effects/metabolism/pathology MH - Mice MH - *Heparinoids/pharmacology/administration & dosage/therapeutic use MH - *Homeostasis/drug effects MH - Mice, Inbred BALB C MH - *Dermatitis/drug therapy/etiology/pathology/metabolism MH - *Skin Cream/pharmacology MH - Filaggrin Proteins MH - *Inflammation/drug therapy/pathology MH - Cytokines/metabolism/genetics MH - Disease Models, Animal MH - Humans MH - Keratinocytes/metabolism/drug effects PMC - PMC13705079 OTO - NOTNLM OT - atopic dermatitis OT - heparinoid cream OT - inflammation OT - skin barrier COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/21 CRDT- 2026/10/27 10:41 PHST- 2026/09/21 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ijms27209360 [pii] AID - ijms-27-09360 [pii] AID - 10.3390/ijms27209360 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 21;27(20):9360. doi: 10.3390/ijms27209360. PMID- 42942123 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 21 TI - Targeting Redox Homeostasis with Arvanil Is Preferentially Cytotoxic to Glioma Stem Cells. LID - 10.3390/ijms27209358 [doi] LID - 9358 AB - Glioma stem cells (GSCs) are cancer stem cells in glioblastoma (GBM) that are resistant to both radiotherapy and chemotherapy and contribute to tumor recurrence. Therefore, the development of GSC-targeted therapies is urgently needed to improve the poor prognosis of GBM patients. Given the growing interest in redox homeostasis, which is closely linked to mitochondrial metabolism supporting the unique properties of GSCs, we focused on capsaicin, a widely used exogenous calcium channel agonist, and its structural analogs as candidate compounds capable of disrupting redox homeostasis in GSCs. Based on a comprehensive evaluation of the effects of the candidate compounds, we found that the capsaicin analog arvanil selectively induced cell death in GSCs with high selectivity over differentiated GSCs at concentrations that were non-toxic to normal cells. Arvanil-induced cell death was associated with a preferential elevation in intracellular reactive oxygen species (ROS) levels in GSCs over those in differentiated GSCs and was suppressed by a Ca(2+) chelator and a mitochondria-targeted antioxidant. These results suggest that arvanil preferentially induced GSC death in association with intracellular Ca(2+) dysregulation and mitochondria-related oxidative stress, highlighting potential vulnerabilities in the redox homeostasis of GSCs and supporting further investigations on arvanil as a feasible therapeutic approach for GBM. FAU - Nakamura, Kazuki AU - Nakamura K AD - Department of Molecular Cancer Science, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata 990-9585, Japan. AD - Department of Neurosurgery, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata 990-9585, Japan. FAU - Takenouchi, Senri AU - Takenouchi S AUID- ORCID: 0009-0008-6850-8763 AD - Department of Molecular Cancer Science, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata 990-9585, Japan. FAU - Nakagawa-Saito, Yurika AU - Nakagawa-Saito Y AUID- ORCID: 0000-0002-9904-8842 AD - Department of Molecular Cancer Science, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata 990-9585, Japan. FAU - Ito, Yasufumi AU - Ito Y AD - Department of Molecular Cancer Science, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata 990-9585, Japan. AD - Department of Obstetrics and Gynecology, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata 990-9585, Japan. FAU - Mitobe, Yuta AU - Mitobe Y AD - Department of Molecular Cancer Science, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata 990-9585, Japan. AD - Department of Neurosurgery, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata 990-9585, Japan. FAU - Suzuki, Shuhei AU - Suzuki S AUID- ORCID: 0000-0003-1975-0146 AD - Department of Molecular Cancer Science, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata 990-9585, Japan. AD - Department of Clinical Oncology, Yamagata Prefectural Shinjo Hospital, 720-1 Kanazawa, Shinjo 996-8585, Japan. FAU - Togashi, Keita AU - Togashi K AD - Department of Molecular Cancer Science, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata 990-9585, Japan. AD - Department of Ophthalmology and Visual Sciences, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata 990-9585, Japan. FAU - Sugai, Asuka AU - Sugai A AD - Department of Molecular Cancer Science, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata 990-9585, Japan. FAU - Sonoda, Yukihiko AU - Sonoda Y AD - Department of Neurosurgery, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata 990-9585, Japan. FAU - Kitanaka, Chifumi AU - Kitanaka C AD - Department of Molecular Cancer Science, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata 990-9585, Japan. FAU - Okada, Masashi AU - Okada M AUID- ORCID: 0000-0002-6333-6076 AD - Department of Molecular Cancer Science, Yamagata University School of Medicine, 2-2-2 Iida-nishi, Yamagata 990-9585, Japan. LA - eng GR - 26K11988/Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan/ GR - 26K10160/Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Reactive Oxygen Species) RN - SY7Q814VUP (Calcium) SB - IM MH - Humans MH - *Neoplastic Stem Cells/drug effects/metabolism/pathology MH - Reactive Oxygen Species/metabolism MH - Oxidation-Reduction/drug effects MH - *Homeostasis/drug effects MH - Calcium/metabolism MH - *Glioma/metabolism/pathology/drug therapy MH - Mitochondria/metabolism/drug effects MH - Cell Line, Tumor MH - *Brain Neoplasms/metabolism/drug therapy/pathology MH - Oxidative Stress/drug effects PMC - PMC13705373 OTO - NOTNLM OT - calcium OT - glioblastoma multiforme OT - reactive oxygen species OT - redox homeostasis OT - tumor-initiating cells COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/21 CRDT- 2026/10/27 10:41 PHST- 2026/08/25 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ijms27209358 [pii] AID - ijms-27-09358 [pii] AID - 10.3390/ijms27209358 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 21;27(20):9358. doi: 10.3390/ijms27209358. PMID- 42942113 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 21 TI - Tumor-Centric Immune Crosstalk in Colorectal and Gastric Cancer: Molecular Mechanisms Linking Tumor Cells to Macrophages and CD8+ T Lymphocytes. LID - 10.3390/ijms27209346 [doi] LID - 9346 AB - Digestive malignancies remain a major cause of cancer-related mortality, with tumor progression (TP) being strongly influenced by the complex interactions between tumor cells (TCs) and their microenvironment. This review summarizes recently described mechanisms of interactions between tumor cells, macrophages and CD8+ T lymphocytes in colorectal and gastric cancer. PubMed and Web of Science were searched for studies published between 2020 and 2026, with the final search being conducted on 8 July 2026. Experimental and clinical studies, together with relevant reviews, were considered to examine these interactions and their therapeutic implications. The evidence shows a bilateral crosstalk in which tumor cells influence immune cell phenotype and function, while immune cells can further support or restrict tumor progression. These interactions involve receptor-ligand systems and paracrine signaling, followed by changes in intracellular pathways regulating proliferation, invasion, macrophage polarization and CD8+ T-cell exhaustion. Considerable methodological heterogeneity among studies limits a direct comparison of the reported mechanisms. Several therapeutic approaches targeting these interactions are currently being investigated in early-phase clinical trials. However, many components of tumor-immune crosstalk and their connections remain incompletely understood. A further characterization of these mechanisms may help identify therapeutic strategies able to interfere with the immunosuppressive microenvironment of colorectal and gastric cancers. FAU - Pascu, Andrei AU - Pascu A AUID- ORCID: 0009-0000-7968-7172 AD - Department of Morpho-Functional Sciences I, "Grigore T. Popa" University of Medicine and Pharmacy Iasi, 16 University Street, 700115 Iasi, Romania. AD - Pathology Department, "Sf. Spiridon" University Emergency County Hospital, 1 Independentei Avenue, 700111 Iasi, Romania. FAU - Ianole, Victor AU - Ianole V AUID- ORCID: 0000-0002-2191-6697 AD - Department of Morpho-Functional Sciences I, "Grigore T. Popa" University of Medicine and Pharmacy Iasi, 16 University Street, 700115 Iasi, Romania. AD - Pathology Department, "Prof. Dr. Nicolae Oblu" Emergency Clinical Hospital, 2 Ateneului Street, 700309 Iasi, Romania. FAU - Danciu, Mihai AU - Danciu M AUID- ORCID: 0000-0002-0194-7832 AD - Department of Morpho-Functional Sciences I, "Grigore T. Popa" University of Medicine and Pharmacy Iasi, 16 University Street, 700115 Iasi, Romania. AD - Pathology Department, "Sf. Spiridon" University Emergency County Hospital, 1 Independentei Avenue, 700111 Iasi, Romania. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - Humans MH - *CD8-Positive T-Lymphocytes/immunology MH - *Stomach Neoplasms/immunology/pathology MH - Tumor Microenvironment/immunology MH - *Colorectal Neoplasms/immunology/pathology MH - *Macrophages/immunology MH - Animals MH - T-Cell Exhaustion PMC - PMC13704946 OTO - NOTNLM OT - CD8+ T-cells OT - colorectal cancer OT - gastric cancer OT - immune evasion OT - tumor microenvironment OT - tumor-associated macrophages OT - tumor-immune crosstalk COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/21 CRDT- 2026/10/27 10:41 PHST- 2026/09/20 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ijms27209346 [pii] AID - ijms-27-09346 [pii] AID - 10.3390/ijms27209346 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 21;27(20):9346. doi: 10.3390/ijms27209346. PMID- 42942102 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 21 TI - Volatile Anesthetic-Based Combination Strategies Against Ischemia-Reperfusion Injury: Experimental Evidence, Molecular Mechanisms, and Translational Limitations. LID - 10.3390/ijms27209335 [doi] LID - 9335 AB - Ischemia-reperfusion (I/R) injury is a complex pathological process involving adenosine triphosphate (ATP) depletion, calcium overload, excessive reactive oxygen species generation, mitochondrial dysfunction, inflammatory activation, and regulated cell death. Volatile anesthetics, including sevoflurane, desflurane, and isoflurane, may modulate several of these processes through antioxidant and prosurvival signaling, suppression of inflammatory pathways, and regulation of apoptosis. Because I/R injury involves interconnected mechanisms, combining interventions that act on different targets may offer additional protection. Experimental studies have investigated volatile anesthetics together with fullerenol C60, ischemic conditioning, and selected pharmacological interventions. Other combination strategies involving ozone conditioning and boric acid have also been evaluated in experimental models, although these have not established the effects of combining either intervention with a volatile anesthetic. This narrative review examines the molecular mechanisms and organ-specific evidence underlying these approaches, with particular attention to study design, treatment timing, and the interpretation of combined effects. While some combinations have shown greater protection than individual interventions, the findings remain heterogeneous, and formal analyses distinguishing additivity from synergy are generally lacking. Clinical evidence is limited, and further studies are needed to establish whether these experimental findings can be translated into meaningful patient outcomes. FAU - Sengel, Necmiye AU - Sengel N AD - Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Gazi University, Ankara 06490, Turkiye. FAU - Arslan, Mustafa AU - Arslan M AUID- ORCID: 0000-0003-4882-5063 AD - Department of Anesthesiology and Reanimation, Faculty of Medicine, Gazi University, Ankara 06560, Turkiye. AD - Life Sciences Application and Research Center, Gazi University, Ankara 06830, Turkiye. AD - Laboratory Animal Breeding and Experimental Research Center (GUDAM), Gazi University, Ankara 06510, Turkiye. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Anesthetics, Inhalation) RN - 38LVP0K73A (Sevoflurane) SB - IM MH - Humans MH - Animals MH - *Reperfusion Injury/drug therapy/metabolism/prevention & control MH - *Anesthetics, Inhalation/therapeutic use/pharmacology MH - Sevoflurane/therapeutic use PMC - PMC13704892 OTO - NOTNLM OT - boric acid OT - combination therapy OT - desflurane OT - fullerenol C60 OT - ischemia-reperfusion injury OT - ischemic conditioning OT - ozone OT - sevoflurane OT - volatile anesthetics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/21 CRDT- 2026/10/27 10:41 PHST- 2026/09/22 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ijms27209335 [pii] AID - ijms-27-09335 [pii] AID - 10.3390/ijms27209335 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 21;27(20):9335. doi: 10.3390/ijms27209335. PMID- 42942098 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 20 TI - Genome-Wide Identification and Analysis of the Perennial Ryegrass (Lolium perenne L.) WRKY Family Associated with Heat Stress. LID - 10.3390/ijms27209332 [doi] LID - 9332 AB - Perennial ryegrass, the most widely cultivated forage and turfgrass, exhibits a relatively poor temperature tolerance, which restricts its distribution and utilization in warmer regions. WRKY transcription factors regulate diverse biological processes in plants, including development and stress responses. Here, we report the comprehensive identification of 97 WRKY genes (LpWRKYs) in perennial ryegrass. The LpWRKYs were unevenly distributed across seven chromosomes, with one gene anchored to a scaffold. These genes were classified into three major groups and five subgroups based on phylogenetic relationships and structural domain diversity. All LpWRKYs contained either one or two WRKY domains and the LpWRKYs within the same group had similar motif compositions. A total of 12 tandem duplicates and 27 segmental duplicates were identified, suggesting that gene duplication might have contributed to the expansion of the LpWRKY gene family. Ka/Ks analysis revealed that all duplicated LpWRKY gene pairs were subjected to purifying selection during the evolution. Co-linearity analysis showed strong co-linearity between LpWRKYs and OsWRKYs from rice. Cis-acting elements in the LpWRKY promoters were related to growth and development, phytohormone responses, and stress responses. RNA-seq and qRT-PCR analysis revealed that the expression levels of LpWRKY5/16/33/35 were significantly up-regulated, while LpWRKY21/92 were significantly down-regulated in roots under heat stress. Additionally, LpWRKY12/21/33/35/43/89/92/96 displayed significant down-regulation in leaves under heat stress. It was suggested that these LpWRKYs were potentially associated with the heat-stress response in perennial ryegrass. Notably, LpWRKY33 and LpWRKY35 exhibited distinct tissue-specific transcriptional patterns in response to heat stress, implying their participation in complex tissue-dependent regulatory networks. Protein interaction prediction further suggested that LpWRKY96 may function as a potential central regulator in the heat response signaling network. Taken together, this study provides clues into the regulatory roles of the WRKY gene family in perennial ryegrass. FAU - Chen, Wei AU - Chen W AD - College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China. AD - College of Landscape Architecture, Jiangsu Vocational College of Agriculture and Forestry, Jurong 212400, China. FAU - Song, Nan AU - Song N AD - College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China. FAU - Zhang, Jing AU - Zhang J AD - College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China. FAU - Xu, Bin AU - Xu B AUID- ORCID: 0000-0002-9701-7583 AD - College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China. FAU - Chi, Yingjun AU - Chi Y AUID- ORCID: 0000-0003-3547-3476 AD - College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China. LA - eng GR - 32301492/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Transcription Factors) RN - 0 (Plant Proteins) SB - IM MH - *Lolium/genetics MH - *Transcription Factors/genetics/metabolism MH - *Plant Proteins/genetics/metabolism MH - Phylogeny MH - Gene Expression Regulation, Plant MH - *Heat-Shock Response/genetics MH - Multigene Family MH - Gene Duplication MH - Promoter Regions, Genetic MH - Genome, Plant MH - Chromosome Mapping PMC - PMC13704931 OTO - NOTNLM OT - WRKY transcription factors OT - gene family OT - heat stress OT - perennial ryegrass COIS- The authors declare no conficts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/20 CRDT- 2026/10/27 10:41 PHST- 2026/09/23 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - ijms27209332 [pii] AID - ijms-27-09332 [pii] AID - 10.3390/ijms27209332 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 20;27(20):9332. doi: 10.3390/ijms27209332. PMID- 42942092 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 20 TI - Model-Dependent EV Biomarker Signatures: From Culture Systems to Translational Perspectives. LID - 10.3390/ijms27209325 [doi] LID - 9325 AB - Extracellular Vesicles (EVs) have emerged as key mediators of intercellular communication and, more recently, as promising biomarkers for disease diagnosis, prognosis, and therapeutic monitoring. In vitro cellular models are essential tools for investigating EV biogenesis, cargo composition, and biological functions. This review provides an overview of EV biology, including their classification, molecular content, biological functions and clinical relevance. It summarizes the main two-dimensional (2D) and three-dimensional (3D) culture systems currently used in EV research. The literature suggests that 3D models more closely recapitulate in vivo architecture, whereas conventional 2D cultures may introduce biological biases that affect experimental outcomes. While these aspects are presented from a simplified perspective to provide necessary background, the review primarily evaluates whether and how culture model selection influences EV production and features. In the final sections, we summarize and discuss evidence comparing EV yield, molecular cargo, functional properties, and biomarker profiles from 2D and 3D systems, and how they reflect in vivo settings. By examining these differences, we discuss their implications for interpreting EV-based studies and the importance of model choice in identifying and validating reliable EV biomarkers. FAU - di Fazio, Giulia AU - di Fazio G AUID- ORCID: 0009-0002-8760-5928 AD - Department of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, Italy. FAU - Poppa, Giuseppina AU - Poppa G AUID- ORCID: 0000-0003-2067-9615 AD - Department of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, Italy. FAU - D'Ascenzo, Sandra AU - D'Ascenzo S AUID- ORCID: 0000-0003-2820-2459 AD - Department of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, Italy. FAU - Dolo, Vincenza AU - Dolo V AUID- ORCID: 0000-0003-0424-6676 AD - Department of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, Italy. FAU - Taverna, Simona AU - Taverna S AUID- ORCID: 0000-0002-2192-8938 AD - Institute of Translational Pharmacology (IFT), National Research Council (CNR), 90146 Palermo, Italy. FAU - Giusti, Ilaria AU - Giusti I AD - Department of Life, Health and Environmental Sciences, University of L'Aquila, 67100 L'Aquila, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261020 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Biomarkers) SB - IM MH - Humans MH - *Extracellular Vesicles/metabolism MH - *Biomarkers/metabolism MH - Animals MH - Cell Culture Techniques/methods MH - *Models, Biological MH - Translational Research, Biomedical PMC - PMC13705121 OTO - NOTNLM OT - biomarkers OT - cancer OT - culture models OT - extracellular vesicles OT - spheroids COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/20 CRDT- 2026/10/27 10:41 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - ijms27209325 [pii] AID - ijms-27-09325 [pii] AID - 10.3390/ijms27209325 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 20;27(20):9325. doi: 10.3390/ijms27209325. PMID- 42942089 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 20 TI - Epigenetic Mechanisms in Neurodegenerative Diseases: From Genetic Regulation to Therapeutic Potential. LID - 10.3390/ijms27209322 [doi] LID - 9322 AB - Neurodegenerative diseases, including Alzheimer's disease (AD), Parkinson's disease (PD), and amyotrophic lateral sclerosis (ALS), are multifactorial disorders in which genetic susceptibility alone cannot account for sporadic onset, clinical diversity, or age-dependent progression. Epigenetic mechanisms-DNA methylation, histone modifications, non-coding RNAs, and m6A RNA methylation-bridge environmental and intracellular cues to stable gene expression changes. Emerging evidence associates epigenetic dysregulation with amyloid-beta/tau pathology in AD, alpha-synuclein pathology in PD, and TDP-43/C9orf72-associated toxicity in ALS, while functional studies suggest that selected epigenetic alterations may contribute to these processes. In this narrative review, we distinguish associative, functional, and mechanistic evidence using claim-specific criteria based on experimental perturbation, direct molecular assessment, and pathway-dependence or rescue testing, while considering the study system. We further evaluate the translational promise of epigenetic biomarkers in liquid biopsies, while emphasizing the major barriers that separate bench findings from bedside application, including poor target specificity, limited blood-brain barrier penetration, and the absence of biomarker-guided patient stratification. By integrating pathological mechanisms, biomarker development, and therapeutic strategies, this review argues that future epigenetic research in neurodegeneration must prioritize causal validation, CNS-penetrant drug development, and prospective trials integrating epigenetic profiling to achieve meaningful clinical impact. FAU - Lv, Xiaoman AU - Lv X AD - College of Pharmaceutical Science, Dali University, Dali 671003, China. FAU - Huang, Mincong AU - Huang M AD - College of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming 650500, China. FAU - Long, Wenjuan AU - Long W AD - Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Neuropsychiatric Diseases, Yunnan University of Chinese Medicine, Kunming 650500, China. FAU - Li, Xinyao AU - Li X AD - The First Clinical Medical College, Yunnan University of Chinese Medicine, Kunming 650500, China. FAU - Hu, Zhongyang AU - Hu Z AD - Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Neuropsychiatric Diseases, Yunnan University of Chinese Medicine, Kunming 650500, China. FAU - Feng, Yujun AU - Feng Y AD - Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Neuropsychiatric Diseases, Yunnan University of Chinese Medicine, Kunming 650500, China. FAU - Du, Jiarong AU - Du J AD - Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Neuropsychiatric Diseases, Yunnan University of Chinese Medicine, Kunming 650500, China. FAU - Long, Qiongrong AU - Long Q AD - Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Neuropsychiatric Diseases, Yunnan University of Chinese Medicine, Kunming 650500, China. FAU - Chen, Yanxin AU - Chen Y AD - Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Neuropsychiatric Diseases, Yunnan University of Chinese Medicine, Kunming 650500, China. FAU - Shi, Mingqin AU - Shi M AUID- ORCID: 0009-0001-9700-1549 AD - The First Clinical Medical College, Yunnan University of Chinese Medicine, Kunming 650500, China. FAU - Qin, Dongdong AU - Qin D AD - Key Laboratory of Traditional Chinese Medicine for Prevention and Treatment of Neuropsychiatric Diseases, Yunnan University of Chinese Medicine, Kunming 650500, China. LA - eng GR - YPKLS2025-029/Yunnan Key Laboratory of Integrated Traditional Chinese and Western Medicine for Chronic Dis-ease in Prevention and Treatment/ GR - KUST-KH2022017Y/Kunming University of Science and Technology & the First People's Hospital of Yunnan Province Joint Special Project on Medical Research/ GR - 202401AY070001-135/Yunnan Provincial Department of Science and Technology-Kunming Medical University Joint Special Project/ PT - Journal Article PT - Review DEP - 20261020 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Biomarkers) RN - 0 (Histones) SB - IM MH - Humans MH - *Epigenesis, Genetic MH - *Neurodegenerative Diseases/genetics/therapy/metabolism MH - Animals MH - DNA Methylation MH - Gene Expression Regulation MH - RNA Methylation MH - Biomarkers/metabolism MH - Histones/metabolism MH - Epitranscriptome PMC - PMC13705238 OTO - NOTNLM OT - DNA methylation OT - RNA methylation OT - epigenetics OT - histone modifications OT - neurodegenerative diseases OT - non-coding RNAs COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/20 CRDT- 2026/10/27 10:41 PHST- 2026/09/18 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - ijms27209322 [pii] AID - ijms-27-09322 [pii] AID - 10.3390/ijms27209322 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 20;27(20):9322. doi: 10.3390/ijms27209322. PMID- 42942087 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 20 TI - Condition-Controlled Divergent Reactivity of Nitroaldol Isatin Adducts with Phenols: Friedel-Crafts Alkylation, Skeletal Rearrangement, and Spirocyclic Oxime Formation. LID - 10.3390/ijms27209320 [doi] LID - 9320 AB - We describe the condition-controlled divergent reactivity of nitroaldol isatin adducts with phenols. Under methanesulfonic acid, nitroalcohols undergo Friedel-Crafts alkylation to afford 3-aryl-3-(nitromethyl)indolin-2-ones in yields of up to 99%. Prolonged acidic treatment of selected Friedel-Crafts adducts leads to an unusual C3-to-C2 skeletal rearrangement, providing tetracyclic chromenoindolones in moderate yields. In contrast, treatment of the alkylation products with KOH in aqueous ethanol promotes intramolecular cyclization to spirocyclic hydroxyimino derivatives. This transformation afforded 16 compounds in 80-96% yields, whereas five new chromenoindolones were obtained in 40-56% yields. In total, 51 new compounds were synthesized, comprising 30 Friedel-Crafts alkylation products, 16 spirocyclic oxime derivatives, and five new chromenoindolones. The structures of representative products 8aa and 13ab were established by X-ray crystallography. The Friedel-Crafts reaction tolerates a range of alkyl, halogen, hydroxy, alkoxy, and N-substituted substrates, although several strongly electron-withdrawing substituents and alpha-naphthol were not compatible with the reaction conditions. Scalability was demonstrated on a 10 mmol scale for the synthesis of 7ae and its subsequent conversion to 13ae. The results demonstrate that nitroaldol isatin adducts can undergo markedly different reaction pathways depending on the reaction conditions, providing access to structurally distinct indole-containing frameworks. FAU - Arutiunov, Nikolai A AU - Arutiunov NA AUID- ORCID: 0000-0003-2675-9093 AD - Department of Chemistry, North Caucasus Federal University, 1a Pushkin St., 355017 Stavropol, Russia. FAU - Shtal, Daria A AU - Shtal DA AD - Department of Chemistry, North Caucasus Federal University, 1a Pushkin St., 355017 Stavropol, Russia. FAU - Tolstov, Kirill V AU - Tolstov KV AD - Department of Chemistry, North Caucasus Federal University, 1a Pushkin St., 355017 Stavropol, Russia. FAU - Korneeva, Anastasia A AU - Korneeva AA AD - Department of Chemistry, North Caucasus Federal University, 1a Pushkin St., 355017 Stavropol, Russia. FAU - Petrova, Olga V AU - Petrova OV AD - Department of Chemistry, North Caucasus Federal University, 1a Pushkin St., 355017 Stavropol, Russia. FAU - Zatsepilina, Anna M AU - Zatsepilina AM AUID- ORCID: 0000-0001-9053-592X AD - Department of Chemistry, North Caucasus Federal University, 1a Pushkin St., 355017 Stavropol, Russia. FAU - Aksenov, Nicolai A AU - Aksenov NA AUID- ORCID: 0000-0002-7125-9066 AD - Department of Chemistry, North Caucasus Federal University, 1a Pushkin St., 355017 Stavropol, Russia. FAU - Ovcharov, Sergei N AU - Ovcharov SN AD - Department of Chemistry, North Caucasus Federal University, 1a Pushkin St., 355017 Stavropol, Russia. FAU - Aksenov, Alexander V AU - Aksenov AV AUID- ORCID: 0000-0002-6644-9949 AD - Department of Chemistry, North Caucasus Federal University, 1a Pushkin St., 355017 Stavropol, Russia. LA - eng GR - 25-23-00961/Russian Science Foundation/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 82X95S7M06 (Isatin) RN - 0 (Phenols) RN - 0 (Oximes) RN - 0 (Spiro Compounds) SB - IM MH - Alkylation MH - *Isatin/chemistry MH - *Phenols/chemistry MH - *Oximes/chemistry/chemical synthesis MH - *Spiro Compounds/chemistry MH - Crystallography, X-Ray MH - Molecular Structure MH - Cyclization PMC - PMC13704967 OTO - NOTNLM OT - Friedel-Crafts alkylation OT - chromenoindolones OT - divergent reactivity OT - isatin OT - nitroaldol adducts OT - skeletal rearrangement OT - spirocyclic oximes COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/20 CRDT- 2026/10/27 10:41 PHST- 2026/09/29 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - ijms27209320 [pii] AID - ijms-27-09320 [pii] AID - 10.3390/ijms27209320 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 20;27(20):9320. doi: 10.3390/ijms27209320. PMID- 42942084 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 20 TI - Release Is Not Dose: Speciation, Bioavailability and Cellular Signalling of Metal Ions from Orthodontic and Prosthetic Alloys in the Oral Environment. LID - 10.3390/ijms27209318 [doi] LID - 9318 AB - Metal release from orthodontic and prosthetic alloys is measured almost universally as total elemental concentration in artificial or whole saliva and declared safe by comparison with dietary intake, drinking-water limits or toxic thresholds. This critical review argues that the comparison, a legitimate screen for systemic exposure, becomes a category error when used to exclude local mucosal effects. It traces the release literature's reasoning since 1986 and shows that the extraction medium, not the alloy, determines the quantity and chemical form of what is measured. It then maps oral species onto cellular uptake routes and signalling pathways. Biological effect depends on species (aquo-ion, low-molecular-mass complex, protein adduct, oxyanion or particle); species dictate entry via divalent metal transporter 1, anion transporters, receptor-mediated endocytosis, membrane interaction or phagocytosis, thereby selecting between Toll-like receptor 4, NOD-like receptor family pyrin domain-containing 3, caspase-8, hypoxia-inducible factor 1alpha and non-apoptotic death programmes; and whole-saliva totals do not describe biofilm, crevicular or intracellular exposure. Oral speciation evidence rests on two nuclear magnetic resonance titrations and one chromium(VI) separation, and most mechanisms are borrowed from other tissues. A speciation-aware exposure model, presented as a hypothesis-generating framework, a minimum reporting set and prioritised experiments are proposed. FAU - Mikulewicz, Marcin AU - Mikulewicz M AUID- ORCID: 0000-0001-5754-0284 AD - Department of Dentofacial Orthopaedics and Orthodontics, Division of Facial Abnormalities, Wroclaw Medical University, Krakowska 26, 50-425 Wroclaw, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261020 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Alloys) RN - 0 (Metals) RN - 0 (Ions) SB - IM MH - Humans MH - Signal Transduction MH - *Alloys/chemistry MH - *Metals/metabolism MH - Animals MH - Biological Availability MH - *Mouth/metabolism MH - Saliva/chemistry MH - *Orthodontic Appliances/adverse effects MH - Ions PMC - PMC13705254 OTO - NOTNLM OT - Toll-like receptor 4 OT - bioaccessibility OT - chemical speciation OT - metal ion release OT - nickel OT - orthodontic alloys COIS- The author is the Guest Editor of this Special Issue and an Editorial Board Member of IJMS. The manuscript was handled by an independent editor, and the author was not involved in its editorial processing or peer review. The author declares no other conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/20 CRDT- 2026/10/27 10:41 PHST- 2026/09/24 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - ijms27209318 [pii] AID - ijms-27-09318 [pii] AID - 10.3390/ijms27209318 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 20;27(20):9318. doi: 10.3390/ijms27209318. PMID- 42942071 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 20 TI - L1CAM-Enriched Extracellular Vesicle MicroRNAs in Mild Cognitive Impairment and Alzheimer's Disease: An Exploratory Study. LID - 10.3390/ijms27209301 [doi] LID - 9301 AB - Early diagnosis of Alzheimer's disease (AD) and the identification of reliable molecular biomarkers remain critical clinical needs. In this context, extracellular vesicles (EVs) enriched by L1CAM immunocapture-L1CAM-enriched EVs-a fraction widely used to obtain material of putative neuronal origin, have been proposed as a minimally invasive source of information on brain molecular changes. We conducted next-generation sequencing (NGS) of microRNAs (miRNAs) from L1CAM-enriched EVs isolated from the plasma of 11 AD patients, 11 mild cognitive impairment (MCI) individuals, and 14 healthy controls (CTRs) to identify a candidate molecular signature. Our analysis identified five differentially expressed miRNAs-miR-423-5p, miR-122-5p, miR-151b, miR-16-5p, and miR-149-3p-in MCI individuals. Three of these-miR-423-5p, miR-122-5p and miR-151b-were higher in MCI and lower in AD, a pattern that differentiated the two clinically defined groups. Furthermore, enrichment analysis of the predicted targets of the differentially expressed miRNAs identified processes relevant to neurodegeneration, including synaptic organisation, cytoskeletal dynamics, and protein degradation. Overall, these preliminary findings identify blood-based L1CAM-enriched EV miRNAs as candidates for further evaluation in cognitive impairment. While validation in larger, biomarker-confirmed and independent cohorts is required, this exploratory study provides data that may guide the design of future biomarker studies. FAU - De Benedittis, Selene AU - De Benedittis S AUID- ORCID: 0000-0001-6340-8888 AD - Institute for Biomedical Research and Innovation (IRIB), Italian National Research Council (CNR), Loc. Burga 44, 87050 Mangone, Italy. FAU - Spadafora, Patrizia AU - Spadafora P AD - Institute for Biomedical Research and Innovation (IRIB), Italian National Research Council (CNR), Loc. Burga 44, 87050 Mangone, Italy. FAU - Greco, Beatrice Maria AU - Greco BM AD - Institute for Biomedical Research and Innovation (IRIB), Italian National Research Council (CNR), Loc. Burga 44, 87050 Mangone, Italy. AD - Department of Biology, Ecology and Earth Sciences (DiBEST) University of Calabria, 87036 Rende, Italy. FAU - Di Palo, Armando AU - Di Palo A AD - Genomix4Life Srl, 84081 Baronissi, Italy. FAU - Gallo, Olivier AU - Gallo O AD - Institute for Biomedical Research and Innovation (IRIB), Italian National Research Council (CNR), Loc. Burga 44, 87050 Mangone, Italy. FAU - Nuzzo, Domenico AU - Nuzzo D AUID- ORCID: 0000-0002-4325-417X AD - Institute for Biomedical Research and Innovation (IRIB), Italian National Research Council (CNR), Via U. La Malfa 153, 90146 Palermo, Italy. FAU - Picone, Pasquale AU - Picone P AUID- ORCID: 0009-0008-2828-1912 AD - Institute for Biomedical Research and Innovation (IRIB), Italian National Research Council (CNR), Via U. La Malfa 153, 90146 Palermo, Italy. FAU - Brunelli, Elvira AU - Brunelli E AUID- ORCID: 0000-0003-3669-1395 AD - Department of Biology, Ecology and Earth Sciences (DiBEST) University of Calabria, 87036 Rende, Italy. FAU - Sesti, Settimio AU - Sesti S AUID- ORCID: 0000-0001-5979-9003 AD - Department of Biology, Ecology and Earth Sciences (DiBEST) University of Calabria, 87036 Rende, Italy. FAU - Montesanto, Alberto AU - Montesanto A AUID- ORCID: 0000-0002-9563-2216 AD - Department of Biology, Ecology and Earth Sciences (DiBEST) University of Calabria, 87036 Rende, Italy. FAU - Passarino, Giuseppe AU - Passarino G AUID- ORCID: 0000-0003-4701-9748 AD - Department of Biology, Ecology and Earth Sciences (DiBEST) University of Calabria, 87036 Rende, Italy. FAU - Risoleo, Katia AU - Risoleo K AD - Regional Neurogenetic Centre (CRN), Department of Primary Care, ASP Catanzaro, 88046 Lamezia Terme, Italy. FAU - Ferrise, Francesca AU - Ferrise F AD - Regional Neurogenetic Centre (CRN), Department of Primary Care, ASP Catanzaro, 88046 Lamezia Terme, Italy. FAU - Colao, Rosanna AU - Colao R AD - Regional Neurogenetic Centre (CRN), Department of Primary Care, ASP Catanzaro, 88046 Lamezia Terme, Italy. FAU - Maletta, Raffaele AU - Maletta R AUID- ORCID: 0000-0002-0758-1835 AD - Regional Neurogenetic Centre (CRN), Department of Primary Care, ASP Catanzaro, 88046 Lamezia Terme, Italy. FAU - Marchese, Giovanna AU - Marchese G AD - Genomix4Life Srl, 84081 Baronissi, Italy. FAU - Cavalcanti, Francesca AU - Cavalcanti F AUID- ORCID: 0000-0001-5769-4582 AD - Institute for Biomedical Research and Innovation (IRIB), Italian National Research Council (CNR), Loc. Burga 44, 87050 Mangone, Italy. LA - eng GR - [DM 1557 11.10.2022]/European Union/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Neural Cell Adhesion Molecule L1) RN - 0 (MicroRNAs) RN - 0 (L1CAM protein, human) RN - 0 (Biomarkers) SB - IM MH - Humans MH - *Alzheimer Disease/genetics/blood/metabolism MH - *Neural Cell Adhesion Molecule L1/metabolism/genetics MH - *MicroRNAs/genetics/metabolism/blood MH - *Cognitive Dysfunction/genetics/blood/metabolism MH - *Extracellular Vesicles/metabolism/genetics MH - Female MH - Biomarkers/blood MH - Male MH - Aged MH - Gene Expression Profiling MH - High-Throughput Nucleotide Sequencing PMC - PMC13704956 OTO - NOTNLM OT - Alzheimer's disease OT - L1CAM-enriched EVs OT - aging OT - biomarkers OT - dementia OT - microRNAs COIS- G.M. and A.D.P. are employees of Genomix4Life S.r.l. their research contributions were provided in the absence of any commercial or financial relationship that could be construed as a potential conflict of interest. The remaining authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/20 CRDT- 2026/10/27 10:41 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - ijms27209301 [pii] AID - ijms-27-09301 [pii] AID - 10.3390/ijms27209301 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 20;27(20):9301. doi: 10.3390/ijms27209301. PMID- 42942069 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 20 TI - Exploring Electron-Donating Thioether Substituents in Arylidene-Imidazolones Towards Environment-Sensitive Fluorogens. LID - 10.3390/ijms27209299 [doi] LID - 9299 AB - In the present study, we explore the introduction of thioether substituents as electron-donating groups in arylidene-imidazolones, a well-established class of GFP chromophore analogs. A series of imidazolones bearing various sulfur-nitrogen substitution patterns was synthesized and converted into the corresponding conformationally locked derivatives with a difluoroboryl bridge. Systematic photophysical study across solvents of different polarity revealed high environment-dependent fluorescence variation for several compounds. A single compound exhibited a remarkable pH-gated fluorescence turn-on (>200-fold) upon protonation, functioning as a pH sensor. Due to its solvent-dependent fluorescence intensity variation, one of the proposed compounds can be applied as a polarity sensor for the selective fluorescent imaging of the endoplasmic reticulum in living cells. FAU - Rudik, Daniil I AU - Rudik DI AD - Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia. FAU - Myasnyanko, Ivan N AU - Myasnyanko IN AD - Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia. AD - Laboratory of Medicinal Substances Chemistry, Institute of Translational Medicine, Pirogov Russian National Research Medical University, Ostrovitianov 1, Moscow 117997, Russia. FAU - Smirnov, Alexander Yu AU - Smirnov AY AUID- ORCID: 0000-0002-1545-6604 AD - Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia. AD - Laboratory of Medicinal Substances Chemistry, Institute of Translational Medicine, Pirogov Russian National Research Medical University, Ostrovitianov 1, Moscow 117997, Russia. FAU - Baranov, Mikhail S AU - Baranov MS AUID- ORCID: 0000-0002-9339-7603 AD - Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia. AD - Laboratory of Medicinal Substances Chemistry, Institute of Translational Medicine, Pirogov Russian National Research Medical University, Ostrovitianov 1, Moscow 117997, Russia. FAU - Bogdanova, Yulia A AU - Bogdanova YA AUID- ORCID: 0000-0003-1745-4204 AD - Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia. AD - Laboratory of Medicinal Substances Chemistry, Institute of Translational Medicine, Pirogov Russian National Research Medical University, Ostrovitianov 1, Moscow 117997, Russia. LA - eng GR - 26-73-00173/Russian Science Foundation/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Imidazoles) RN - 0 (Sulfides) RN - 0 (Fluorescent Dyes) RN - 147336-22-9 (Green Fluorescent Proteins) RN - 0 (imidazolone) RN - 0 (Fluorescent Chemosensor Compounds) RN - 0 (arylidene) RN - 0 (Aza Compounds) RN - 0 (Heterocyclic Compounds, 4 or More Rings) SB - IM MH - *Imidazoles/chemistry MH - *Sulfides/chemistry MH - *Fluorescent Dyes/chemistry MH - Green Fluorescent Proteins/chemistry MH - Humans MH - Hydrogen-Ion Concentration MH - Electrons MH - Fluorescent Chemosensor Compounds MH - Endoplasmic Reticulum/metabolism MH - Aza Compounds MH - Heterocyclic Compounds, 4 or More Rings PMC - PMC13705515 OTO - NOTNLM OT - bioimaging OT - endoplasmic reticulum OT - fluorescent dye OT - fluorescent microscopy OT - fluorogen OT - green fluorescent protein (GFP) COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/20 CRDT- 2026/10/27 10:41 PHST- 2026/09/23 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - ijms27209299 [pii] AID - ijms-27-09299 [pii] AID - 10.3390/ijms27209299 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 20;27(20):9299. doi: 10.3390/ijms27209299. PMID- 42942064 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 19 TI - From Phenotype to Genome: A Critical Evidence-Based Synthesis of Frosty Pod Rot (Moniliophthora roreri) Resistance in Cacao and a Proposed Evidence-Gated Framework to Support Genetic Gain. LID - 10.3390/ijms27209294 [doi] LID - 9294 AB - Frosty pod rot (FPR), caused by Moniliophthora roreri, severely constrains cacao production in the Neotropics, yet evidence supporting molecular breeding for resistance remains fragmented and is often conflated with findings from witches' broom disease. We conducted a structured critical review, not a systematic review, and classified each FPR-related claim with an author-developed, multidimensional evidence classification system (E0-E5) separating directness, genetic replication, mechanistic inference and breeding utility. The evidence supports a quantitative, partial and context-dependent architecture of resistance. The strongest QTL have been re-detected only within the Pound 7 x UF273 pedigree (E2a), and no FPR locus replicated in genetically independent material (E2b) was identified in the literature retrieved and verified. Genomic predictive ability reached 0.60-0.67 under random cross-validation within one clonal panel but averaged approximately 0.16 when models were transferred among related populations; these values answer different questions and are not directly comparable. Host and pathogen transcriptomics provide correlative evidence (E3a), and recombinant M. roreri effectors show biological activity in heterologous or cacao tissues (E3b-i), but no stable genetic manipulation altering FPR in a pod-challenge assay (E4) and no documented breeding utility (E5) were identified. We propose a Phenotyping and Reporting Guideline for FPR (PRG-FPR) and MIGGA, a modular, evidence-gated decision framework illustrated with a retrospective worked example; its effect on realised genetic gain remains to be tested. FAU - Pineda-Rodriguez, Yirlis Yadeth AU - Pineda-Rodriguez YY AD - Laboratorio de Biologia Molecular Aplicada, Facultad de Ciencias Agricolas, Universidad de Cordoba, Monteria 230002, Colombia. FAU - Navas, Juan Jaraba AU - Navas JJ AUID- ORCID: 0000-0002-5826-707X AD - Laboratorio de Biologia Molecular Aplicada, Facultad de Ciencias Agricolas, Universidad de Cordoba, Monteria 230002, Colombia. FAU - Jarma-Orozco, Alfredo AU - Jarma-Orozco A AUID- ORCID: 0000-0002-5821-2183 AD - Laboratorio de Biologia Molecular Aplicada, Facultad de Ciencias Agricolas, Universidad de Cordoba, Monteria 230002, Colombia. FAU - Oviedo-Zumaque, Luis Eliecer AU - Oviedo-Zumaque LE AD - Facultad de Ciencias Basicas, Universidad de Cordoba, Monteria 230002, Colombia. FAU - Rodriguez-Paez, Luis Alfonso AU - Rodriguez-Paez LA AUID- ORCID: 0000-0002-1760-1852 AD - Laboratorio de Biologia Molecular Aplicada, Facultad de Ciencias Agricolas, Universidad de Cordoba, Monteria 230002, Colombia. LA - eng GR - FX23TA-10960C008/United States Department of Agriculture/ PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - *Cacao/genetics/microbiology MH - *Plant Diseases/microbiology/genetics MH - *Disease Resistance/genetics MH - Phenotype MH - Quantitative Trait Loci MH - *Agaricales/pathogenicity MH - *Genome, Plant PMC - PMC13705398 OTO - NOTNLM OT - Moniliophthora roreri OT - Theobroma cacao OT - cacao breeding OT - frosty pod rot OT - functional validation OT - genome-wide association study (GWAS) OT - genomic selection OT - molecular breeding OT - precision breeding OT - quantitative trait loci (QTL) COIS- The authors declare no conflicts of interest. The funders had no role in the design of this study; in the collection, analysis or interpretation of the literature and evidence synthesised; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/19 CRDT- 2026/10/27 10:41 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ijms27209294 [pii] AID - ijms-27-09294 [pii] AID - 10.3390/ijms27209294 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 19;27(20):9294. doi: 10.3390/ijms27209294. PMID- 42942055 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 19 TI - Conditional Proximity Labeling in Complex Biological Systems: Design Principles, Emerging Platforms, and Applications. LID - 10.3390/ijms27209284 [doi] LID - 9284 AB - Proximity labeling (PL) enables covalent capture of biomolecules near a defined target in living systems, providing a route to map molecular proximity in situ. Conventional PL usually relies on a single targeting cue and may therefore lack the selectivity required to resolve defined molecular events or dynamic processes in complex biological systems. Conditional PL addresses this limitation by coupling labeling activity to additional inputs, including interaction-dependent enzyme reconstitution, localization of catalytic modules at cell-cell interfaces, light-regulated catalysis, and multiple orthogonal labeling reactions. These designs allow labeling to respond to specified spatial, temporal, or molecular conditions. We group conditional PL into four designs-split complementation, interface localization, light control, and multiple labeling-based on what activates labeling and what the labels reveal. We examine their use in studying protein complexes, trafficking, and intercellular communication. We also consider what each strategy encodes, the experimental boundaries of the resulting proximity information, and trade-offs among selectivity, labeling efficiency, temporal control, and compatibility with native biological contexts. Remaining challenges include expanding catalyst and probe diversity, improving in vivo compatibility, and integrating conditional PL with single-cell analysis, spatial omics, and high-resolution imaging. FAU - Yang, Jia-Ming AU - Yang JM AD - State Key Laboratory of Medicinal Chemical Biology, School of Medicine and Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, China. FAU - Zhu, Meng-Die AU - Zhu MD AD - School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. FAU - Liu, Shu-Lin AU - Liu SL AUID- ORCID: 0000-0002-1043-4238 AD - State Key Laboratory of Medicinal Chemical Biology, School of Medicine and Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, China. FAU - Yang, Long AU - Yang L AUID- ORCID: 0009-0001-9293-1923 AD - School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. FAU - Wang, Zhi-Gang AU - Wang ZG AD - State Key Laboratory of Medicinal Chemical Biology, School of Medicine and Research Centre for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, China. LA - eng PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - *Staining and Labeling/methods MH - Animals MH - Humans MH - Proteomics/methods PMC - PMC13705194 OTO - NOTNLM OT - conditional proximity labeling OT - intercellular interactions OT - light-controlled proximity labeling OT - orthogonal labeling OT - proximity labeling OT - spatial proteomics OT - split enzymes COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/19 CRDT- 2026/10/27 10:41 PHST- 2026/08/30 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ijms27209284 [pii] AID - ijms-27-09284 [pii] AID - 10.3390/ijms27209284 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 19;27(20):9284. doi: 10.3390/ijms27209284. PMID- 42942054 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 19 TI - Natural Antioxidant-Derived Compounds and Materials as Emerging MALDI Matrices: Applications, Evidence, and Perspectives. LID - 10.3390/ijms27209285 [doi] LID - 9285 AB - Antioxidants constitute a chemically diverse group of compounds that are widely studied in food, natural products, and biological systems, creating a continuing need for analytical methods capable of their reliable characterization. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) provides rapid molecular analysis with minimal sample preparation and has been increasingly applied to low-molecular-weight compounds, natural products, food constituents, and biological samples. However, the analysis of small molecules by MALDI-MS is often limited by matrix-derived background signals, differences in ionization efficiency, heterogeneous crystallization, and difficulties in achieving reliable quantitative measurements, highlighting the need for alternative matrix systems with improved and more controllable analytical properties. In this context, naturally occurring antioxidants are of particular interest because many possess aromatic and conjugated structures, UV-absorbing groups, acid-base and redox properties, and functional groups capable of matrix-analyte interactions, which may support their function as MALDI matrices. This review summarizes the use of MALDI-MS for the analysis of antioxidant compounds in purified samples, plant extracts, food products, and biological materials, while critically evaluating the emerging concept of using naturally occurring antioxidants as alternative MALDI matrices. Particular emphasis is placed on the structural and physicochemical features relevant to matrix performance, including aromaticity, UV absorption, acid-base properties, redox behavior, crystallization, and matrix-analyte interactions. Conventional organic matrices are discussed alongside emerging alternatives, including ionic liquid matrices, nanomaterial-based systems, carbon-based materials, and matrix-free approaches. Importantly, the available evidence indicates that only a limited number of natural antioxidant-derived compounds and materials have been experimentally demonstrated as MALDI matrices, whereas evidence for other candidates remains preliminary or theoretical. Their potential analytical value is further considered in relation to reproducibility, background interference, matrix selectivity, and quantitative performance, together with future opportunities involving rational matrix design, mass spectrometry imaging, and computational approaches. Natural antioxidant-derived compounds and materials therefore represent an emerging and still insufficiently explored class of MALDI matrices that could expand the range of matrix chemistries available for small-molecule analysis, although their analytical value will ultimately depend on compound-specific experimental validation rather than antioxidant activity alone. FAU - Bania, Katarzyna AU - Bania K AD - Medical Science Club, Faculty of Medicine, University of Rzeszow, 35-310 Rzeszow, Poland. FAU - Bartusik-Aebisher, Dorota AU - Bartusik-Aebisher D AUID- ORCID: 0000-0002-5557-5464 AD - Department of Biochemistry and General Chemistry, Faculty of Medicine, University of Rzeszow, 35-310 Rzeszow, Poland. FAU - Dynarowicz, Klaudia AU - Dynarowicz K AUID- ORCID: 0000-0001-6851-5869 AD - Department of Biochemistry and General Chemistry, Faculty of Medicine, University of Rzeszow, 35-310 Rzeszow, Poland. FAU - Smolak, Barbara AU - Smolak B AUID- ORCID: 0009-0009-4275-4648 AD - Department of Diagnostic Imaging and Nuclear Medicine, Faculty of Medicine, University of Rzeszow, 35-310 Rzeszow, Poland. FAU - Aebisher, David AU - Aebisher D AUID- ORCID: 0000-0002-2661-6570 AD - Department of Photomedicine and Physical Chemistry, Faculty of Medicine, University of Rzeszow, 35-310 Rzeszow, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Antioxidants) RN - 0 (Biological Products) SB - IM MH - *Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods MH - *Antioxidants/chemistry/analysis MH - *Biological Products/chemistry/analysis PMC - PMC13705048 OTO - NOTNLM OT - MALDI matrices OT - MALDI-MS OT - MALDI-TOF-MS OT - alternative matrices OT - antioxidants OT - flavonoids OT - low-molecular-weight compounds OT - mass spectrometry imaging OT - natural products OT - phenolic compounds COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/19 CRDT- 2026/10/27 10:41 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ijms27209285 [pii] AID - ijms-27-09285 [pii] AID - 10.3390/ijms27209285 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 19;27(20):9285. doi: 10.3390/ijms27209285. PMID- 42942052 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 19 TI - Lineage-Associated Dichotomous Patterns in Codon Usage of Parasitic Cestodes. LID - 10.3390/ijms27209282 [doi] LID - 9282 AB - Genome remodeling, accompanied by modifications in base composition and translational architecture, may systematically reshape codon usage patterns, contributing to adaptation to changing biological demands through altered translational efficiency. In the present study, codon usage and genomic tRNA profiles were comparatively investigated in species representing two cestode lineages with contrasting genome organizations and biological characteristics. Despite relatively well-preserved amino-acid frequencies, these cestode species exhibited clear inter-lineage divergence in synonymous codon usage. Diphyllobothriideans preferentially utilized C-ending synonymous codons, whereas cyclophyllideans favored the corresponding T-ending alternatives, forming distinct lineage-associated codon dialects. These divergent patterns were consistently retained across coding-sequence datasets ranging from genome-wide to highly curated orthologous sets. Nucleotide composition contributed substantially to codon usage, while multiple lines of evidence indicated additional selective and translational constraints. Genomic tRNA gene pools were greatly reduced in cyclophyllideans, particularly taeniids. However, relationships between synonymous codon preferences and corresponding tRNA gene counts varied among codon families, indicating that their evolutionary associations may not be uniform. Collectively, these findings suggest that distinct codon usage patterns have evolved in cestode lineages with contrasting genomic architectures, shaped by lineage-specific compositional environments and, potentially for certain codon families, translational factors. They provide a framework for investigating how such diversifications contribute to translational adaptation to nutritional and environmental changes. FAU - Kim, Seon-Hee AU - Kim SH AD - Research & Business Development Foundation, The State University of New York, Korea, Incheon 21985, Republic of Korea. FAU - Yang, Dongki AU - Yang D AUID- ORCID: 0000-0002-8550-1888 AD - Department of Physiology and Lee Gil Ya Cancer and Diabetes Institute, Gachon University College of Medicine, Incheon 21999, Republic of Korea. FAU - Bae, Young-An AU - Bae YA AUID- ORCID: 0000-0002-2050-4842 AD - Department of Microbiology and Lee Gil Ya Cancer and Diabetes Institute, Gachon University College of Medicine, Incheon 21999, Republic of Korea. LA - eng GR - RS-2022-NR072519/National Research Foundation of Korea/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 9014-25-9 (RNA, Transfer) RN - 0 (Codon) SB - IM MH - *Codon Usage MH - Animals MH - RNA, Transfer/genetics MH - *Cestoda/genetics/classification MH - Evolution, Molecular MH - *Codon/genetics MH - Phylogeny MH - Base Composition PMC - PMC13705341 OTO - NOTNLM OT - cestodes OT - codon usage bias OT - genome remodeling OT - synonymous codon OT - tRNA repertoire OT - translational adaptation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/19 CRDT- 2026/10/27 10:41 PHST- 2026/09/21 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ijms27209282 [pii] AID - ijms-27-09282 [pii] AID - 10.3390/ijms27209282 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 19;27(20):9282. doi: 10.3390/ijms27209282. PMID- 42942047 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 19 TI - Ferroptosis-Related GPX4 and CD71 and Immune Checkpoint PD-L1 in Cutaneous Melanoma: Associations with Clinicopathologic Features and Survival Outcomes. LID - 10.3390/ijms27209278 [doi] LID - 9278 AB - Ferroptosis-related pathways and immune checkpoint signaling may contribute to melanoma progression, but their clinicopathologic and prognostic interplay remains incompletely characterized. To investigate GPX4, CD71, and PD-L1 expression in primary cutaneous melanoma and evaluate their associations with clinicopathologic features and survival outcomes. This retrospective single-center cohort included 44 patients with primary cutaneous melanoma. PD-L1, GPX4, and CD71 expressions were assessed immunohistochemically. Associations with clinicopathologic parameters were analyzed, and overall survival (OS) and disease-free survival (DFS) were evaluated using Kaplan-Meier and Cox regression analyses. PD-L1, GPX4, and CD71 positivity was were observed in 25.0%, 38.6%, and 36.4% of tumors, respectively. PD-L1 expression was associated with Breslow thickness (p = 0.004), whereas CD71 expression was associated with perineural invasion, Clark level, and disease stage (p = 0.019, p = 0.034, and p = 0.045, respectively). GPX4-positive tumors showed significantly shorter OS (40 vs. 103 months; p = 0.003) and DFS (42 vs. 86 months; p = 0.009). Triple-positive tumors (PD-L1(+)/GPX4(+)/CD71(+)) demonstrated significantly shorter OS and DFS than triple-negative tumors (p = 0.035 and p = 0.040, respectively). In multivariable analysis, GPX4 positivity remained independently associated with poorer OS. GPX4 expression was associated with adverse survival outcomes in cutaneous melanoma, while combined GPX4, CD71, and PD-L1 expression identified a subgroup with particularly unfavorable outcomes. These findings support further investigation of ferroptosis-related markers alongside immune checkpoint pathways as candidate prognostic biomarkers in melanoma. FAU - Guner, Rukiye Yasak AU - Guner RY AD - Department of Dermatology, Faculty of Medicine, Sivas Cumhuriyet University, 58140 Sivas, Turkey. FAU - Yuceer, Ramazan Oguz AU - Yuceer RO AUID- ORCID: 0000-0002-9418-8862 AD - Department of Pathology, Faculty of Medicine, Sivas Cumhuriyet University, 58140 Sivas, Turkey. FAU - Unsal, Ahmet Turan AU - Unsal AT AUID- ORCID: 0009-0003-8696-9583 AD - Department of Dermatology, Artvin State Hospital, 08000 Artvin, Turkey. FAU - Akyol, Melih AU - Akyol M AUID- ORCID: 0000-0001-7912-0651 AD - Department of Dermatology, Faculty of Medicine, Sivas Cumhuriyet University, 58140 Sivas, Turkey. FAU - Egilmez, Hatice Reyhan AU - Egilmez HR AD - Department of Pathology, Faculty of Medicine, Sivas Cumhuriyet University, 58140 Sivas, Turkey. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Antigens, CD) RN - 0 (CD71 antigen) RN - 0 (B7-H1 Antigen) RN - EC 1.11.1.12 (Phospholipid Hydroperoxide Glutathione Peroxidase) RN - 0 (Receptors, Transferrin) RN - 0 (CD274 protein, human) RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - *Melanoma/pathology/metabolism/mortality MH - *Antigens, CD/metabolism/genetics MH - Female MH - *Ferroptosis MH - *Skin Neoplasms/pathology/metabolism/mortality MH - Male MH - *B7-H1 Antigen/metabolism MH - *Phospholipid Hydroperoxide Glutathione Peroxidase/metabolism MH - Middle Aged MH - *Receptors, Transferrin/metabolism MH - Prognosis MH - Aged MH - Retrospective Studies MH - Adult MH - Cutaneous Malignant Melanoma MH - Biomarkers, Tumor/metabolism MH - Disease-Free Survival PMC - PMC13705435 OTO - NOTNLM OT - biomarkers OT - cutaneous melanoma OT - ferroptosis OT - glutathione peroxidase 4 (GPX4) OT - prognosis OT - programmed death-ligand 1 (PD-L1) OT - transferrin receptor 1 (CD71) COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/19 CRDT- 2026/10/27 10:41 PHST- 2026/09/22 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ijms27209278 [pii] AID - ijms-27-09278 [pii] AID - 10.3390/ijms27209278 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 19;27(20):9278. doi: 10.3390/ijms27209278. PMID- 42942046 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 19 TI - Prognostic Value of lncRNA H19 in Gastric Cancer: A Systematic Review and Meta-Analysis. LID - 10.3390/ijms27209276 [doi] LID - 9276 AB - LncRNA H19 is a crucial regulatory player in GC and could operate as an attractive biomarker for patient prognosis. This meta-analysis aims to gauge the link between lncRNA H19 expression levels and patient prognosis, as well as clinicopathological features. HR and 95%CI were utilized to evaluate the connection between lncRNA H19 expression and prognosis, as well as clinicopathological features in GC patients. Bioinformatics analysis, potential molecular mechanisms and drug therapy involving lncRNA H19 were predicted through databases. The expression of lncRNA H19 in GC cells was verified through qRT-PCR. High expression of lncRNA H19 is correlated with a lower overall survival rate in GC patients, as well as TNM stage, lymph node metastasis and tumor invasion. Bioinformatics analysis confirmed that lncRNA H19 is significantly expressed in GC and related to poor prognosis. Function prediction analyses suggested that lncRNA H19 may contribute to GC progression through ceRNA regulatory networks, TFs, and predicted interactions with RBPs. Nutlin-3, PD-0332991, and Topotecan were predicted as preliminary candidate drugs. LncRNA H19 was confirmed to be highly expressed in GC cells. LncRNA H19 might act as a feasible biomarker for prognostic prediction in GC, warranting further validation in clinical studies. FAU - Song, Silu AU - Song S AD - Key Laboratory of Microecology-Immune Regulatory Network and Related Diseases, College of Basic Medical, Jiamusi University, Jiamusi 154007, China. FAU - Li, Yi AU - Li Y AD - Key Laboratory of Microecology-Immune Regulatory Network and Related Diseases, College of Basic Medical, Jiamusi University, Jiamusi 154007, China. FAU - Gao, Chenxia AU - Gao C AD - Key Laboratory of Microecology-Immune Regulatory Network and Related Diseases, College of Basic Medical, Jiamusi University, Jiamusi 154007, China. FAU - Li, Shuai AU - Li S AD - Key Laboratory of Microecology-Immune Regulatory Network and Related Diseases, College of Basic Medical, Jiamusi University, Jiamusi 154007, China. FAU - Jin, Shaojing AU - Jin S AD - Key Laboratory of Microecology-Immune Regulatory Network and Related Diseases, College of Basic Medical, Jiamusi University, Jiamusi 154007, China. FAU - Liu, Shuang AU - Liu S AD - Key Laboratory of Microecology-Immune Regulatory Network and Related Diseases, College of Basic Medical, Jiamusi University, Jiamusi 154007, China. LA - eng GR - DJXSTD202403/Jiamusi University "East Pole" academic team/ GR - 2022-KYYWF-0656/the Heilongjiang Provincial Higher Education Institutions' Basic Research Operating Expenses Project, China/ PT - Journal Article PT - Meta-Analysis PT - Review PT - Systematic Review DEP - 20261019 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (RNA, Long Noncoding) RN - 0 (H19 long non-coding RNA) RN - 0 (Biomarkers, Tumor) SB - IM MH - *RNA, Long Noncoding/genetics MH - *Stomach Neoplasms/genetics/pathology/diagnosis/mortality MH - Humans MH - Prognosis MH - Gene Expression Regulation, Neoplastic MH - *Biomarkers, Tumor/genetics PMC - PMC13705260 OTO - NOTNLM OT - gastric cancer OT - lncRNA H19 OT - meta-analysis OT - prognosis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/19 CRDT- 2026/10/27 10:41 PHST- 2026/02/21 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ijms27209276 [pii] AID - ijms-27-09276 [pii] AID - 10.3390/ijms27209276 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 19;27(20):9276. doi: 10.3390/ijms27209276. PMID- 42942045 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 19 TI - Maternal Plasma Proteomic Signatures Distinguish Preeclampsia from Healthy Pregnancy but Not Early-Onset from Late-Onset Subtypes: A Leakage-Free Machine-Learning and Explainability Study. LID - 10.3390/ijms27209275 [doi] LID - 9275 AB - Early-onset (EOPE) and late-onset (LOPE) preeclampsia are clinically distinct, but their molecular differences are incompletely defined. Using a strictly leakage-free pipeline, we re-examined maternal plasma proteomes of EOPE, LOPE, and healthy pregnancy (HP) and asked whether the subtypes are separable at the proteome level. Label-free LC-MS/MS proteomes comprising 370 proteins from 46 women (HP, n = 18; EOPE, n = 17; LOPE, n = 11) were analysed using limma and fully nested cross-validation, in which feature selection, class balancing, and tuning were confined to the outer training folds; model explanations used SHAP and feature-selection stability. Observed selection frequencies far exceeded a random-selection null (for example, complement factor D was selected in all 15 outer folds, Wilson 95% confidence interval 0.80 to 1.00, against an expected frequency of 0.07), indicating that the stability was not a chance artefact. Discrimination is summarised as the mean +/- standard deviation across the 5-fold x 3-repeat (15) outer test folds; because the single best-performing classifier was chosen on outer-fold performance, the reported area under the curve is modestly optimistic (on average by 0.04, and by up to 0.10 relative to the median of the three classifiers), even though the leakage-free procedure was applied identically to each classifier. Disease-versus-healthy contrasts were strong and concordant, with 10 and eight differentially abundant proteins identified for EOPE and LOPE, respectively, and AUROCs ranging from 0.88 to 0.90. EOPE showed a complement/acute-phase/coagulation signature, whereas LOPE showed an apolipoprotein/lipid signature, with limma, stability analysis, and SHAP yielding convergent findings. By contrast, the EOPE-versus-LOPE comparison identified no differentially abundant proteins and showed no reliable multivariate separation. A post hoc sensitivity analysis (two-sample t-test, two-sided, alpha = 0.05) indicated that the discovery cohort (early-onset n = 17, late-onset n = 11) had 80% power only for very large per-protein standardised differences (Cohen's d >/= 1.13, or d >/= 2.10 after Bonferroni correction across the 370 proteins), and only 24% and 51% power to detect medium (d = 0.5) and large (d = 0.8) effects; the absence of a detectable early-versus-late difference is therefore consistent with limited power for small-to-moderate effects rather than proven biological equivalence. In an independent ELISA cohort (n = 87), EOPE differed from HP for seven of nine analytes, with AUCs of up to 0.82, led by sFlt-1 (fold-changes paralleled these areas under the curve: the largest early-onset median disease-to-control ratios were fibronectin 2.9, soluble fms-like tyrosine kinase-1 2.8 and alpha1-antitrypsin 2.5, whereas placental growth factor decreased to 0.6 and apolipoprotein E was unchanged at 1.0); however, the subtypes again did not separate. On adjustment for maternal age, body mass index and smoking, the leading early-onset markers remained significant (soluble fms-like tyrosine kinase-1, serum amyloid A and fibronectin robust; complement factor D and alpha-1-antitrypsin borderline), whereas maternal chronic hypertension, diabetes and thyroid disease were not recorded and could not be modelled. Benchmarked against soluble fms-like tyrosine kinase-1 (DeLong test), no novel candidate exceeded its discrimination and several were significantly lower; only glycoprotein hormones alpha chain added cross-validated incremental value in early-onset disease (cross-validated area under the curve 0.71 to 0.76), and none improved on soluble fms-like tyrosine kinase-1 in late-onset disease; apolipoprotein E did not validate, and placental growth factor decreased as expected. Plasma proteomics distinguishes preeclampsia from healthy pregnancy and recovers coherent subtype-leaning signatures but provides little evidence of a sharp EOPE-LOPE boundary; the prioritised panel warrants validation in larger cohorts. FAU - Yasar, Seyma AU - Yasar S AUID- ORCID: 0000-0003-1300-3393 AD - Department of Biostatistics and Medical Informatics, Faculty of Medicine, Inonu University, Malatya 44280, Turkiye. FAU - Melekoglu, Rauf AU - Melekoglu R AUID- ORCID: 0000-0001-7113-6691 AD - Department of Obstetrics and Gynecology, Faculty of Medicine, Inonu University, Malatya 44280, Turkiye. FAU - Basak Turkmen, Nese AU - Basak Turkmen N AD - Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Inonu University, Malatya 44280, Turkiye. FAU - Safarova, Aishana AU - Safarova A AD - Department of Obstetrics and Gynecology, Faculty of Medicine, Inonu University, Malatya 44280, Turkiye. FAU - Colak, Cemil AU - Colak C AUID- ORCID: 0000-0001-5406-098X AD - Department of Biostatistics and Medical Informatics, Faculty of Medicine, Inonu University, Malatya 44280, Turkiye. FAU - Alzakari, Sarah A AU - Alzakari SA AD - Department of Computer Sciences, College of Computer and Information Sciences, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia. FAU - Alkhalifa, Amal K AU - Alkhalifa AK AUID- ORCID: 0000-0002-7273-4041 AD - Department of Computer Sciences, College of Computer and Information Sciences, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia. FAU - Yagin, Fatma Hilal AU - Yagin FH AD - Department of Biostatistics and Medical Informatics, Faculty of Medicine, Balikesir University, Balikesir 10145, Turkiye. LA - eng GR - PNURSP2026R721/Princess Nourah bint Abdulrahman University/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Proteome) RN - 0 (Blood Proteins) RN - 0 (Biomarkers) SB - IM MH - Humans MH - Female MH - *Pre-Eclampsia/blood/diagnosis/metabolism MH - Pregnancy MH - *Machine Learning MH - *Proteomics/methods MH - *Proteome/metabolism MH - *Blood Proteins/metabolism MH - Biomarkers/blood MH - Tandem Mass Spectrometry MH - Adult PMC - PMC13704937 OTO - NOTNLM OT - LC-MS/MS OT - LIME OT - SHAP OT - biomarkers OT - data leakage OT - early-onset OT - late-onset OT - machine learning OT - nested cross-validation OT - plasma proteomics OT - preeclampsia COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/19 CRDT- 2026/10/27 10:41 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ijms27209275 [pii] AID - ijms-27-09275 [pii] AID - 10.3390/ijms27209275 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 19;27(20):9275. doi: 10.3390/ijms27209275. PMID- 42942043 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 19 TI - What a Near-Perfect AUROC Does Not Show: Random-Panel and Label-Permutation Controls for a Sepsis Blood-Transcriptome Signature. LID - 10.3390/ijms27209273 [doi] LID - 9273 AB - Sepsis blood-transcriptome signatures are usually judged against healthy controls. Two questions are rarely separated: whether the classifier exceeds chance, and whether its genes outperform an arbitrary list of equal size. We tested both on public microarray data (discovery GSE65682, n = 359; validation GSE57065, n = 53), using the 9983 of 11,222 genes measurable on both platforms. A 10-gene signature was selected by nested cross-validation and applied to validation without refitting. It separated sepsis from health perfectly (area under the receiver operating characteristic curve, AUROC, 1.000). The pipeline exceeded chance: no label permutation in 1000 matched it (p = 0.001). The genes did not outperform random lists: 12.5% of 2000 random 10-gene panels matched or exceeded the signature, as did 67.6% of panels drawn from the same 300 preselected candidates. Against non-infected intensive care patients, cross-fitted AUROC fell to 0.730 (95% confidence interval 0.642-0.818), unchanged after age and sex matching. At a threshold of 0.5, the cross-fitted model called 70 of these 75 patients septic. Selection was unstable: 200 bootstrap resamples gave a median of 6 genes, from 109 distinct genes. A near-perfect AUROC against healthy controls reflects an easy benchmark, not a specific gene list. FAU - Kolev, Mikhail AU - Kolev M AD - Department of Applied Computer Science and Mathematical Modelling, Faculty of Mathematics and Computer Science, University of Warmia and Mazury in Olsztyn, Sloneczna 54, 10-710 Olsztyn, Poland. AD - Department of Mathematics, University of Architecture, Civil Engineering and Geodesy (UACEG), 1 Hristo Smirnenski Blvd., 1164 Sofia, Bulgaria. FAU - Naskinova, Irina AU - Naskinova I AUID- ORCID: 0009-0009-7794-4265 AD - Department of Mathematics, University of Architecture, Civil Engineering and Geodesy (UACEG), 1 Hristo Smirnenski Blvd., 1164 Sofia, Bulgaria. FAU - Lazarova, Meglena AU - Lazarova M AD - Department of Mathematical Modelling and Numerical Methods, Faculty of Applied Mathematics and Informatics, Technical University of Sofia, 8 "Kliment Ohridski" Blvd., 1000 Sofia, Bulgaria. FAU - Klisurov, Velin AU - Klisurov V AUID- ORCID: 0009-0005-8212-6786 AD - Faculty of Mathematics and Informatics, Sofia University "St. Kliment Ohridski", 5 James Bourchier Blvd., 1164 Sofia, Bulgaria. FAU - Kalinov, Hristo AU - Kalinov H AUID- ORCID: 0009-0002-8548-4907 AD - Faculty of Mathematics and Informatics, Sofia University "St. Kliment Ohridski", 5 James Bourchier Blvd., 1164 Sofia, Bulgaria. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - Humans MH - *Sepsis/genetics/blood/diagnosis MH - *Gene Expression Profiling/methods MH - *Transcriptome MH - ROC Curve MH - Area Under Curve MH - Oligonucleotide Array Sequence Analysis PMC - PMC13704978 OTO - NOTNLM OT - benchmark saturation OT - cross-platform validation OT - diagnostic specificity OT - feature transportability OT - host response OT - microarray OT - nested cross-validation OT - selection stability COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/19 CRDT- 2026/10/27 10:41 PHST- 2026/08/15 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ijms27209273 [pii] AID - ijms-27-09273 [pii] AID - 10.3390/ijms27209273 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 19;27(20):9273. doi: 10.3390/ijms27209273. PMID- 42942039 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 19 TI - Precision Pharmacotherapy for Childhood Obesity: Molecular Targets Beyond Single GLP-1 Receptor Agonism and a Framework for Disease Modification. LID - 10.3390/ijms27209269 [doi] LID - 9269 AB - Childhood obesity is a heterogeneous chronic disease shaped by genetic susceptibility, neuroendocrine signaling, energy homeostasis, adipose dysfunction, insulin resistance, and environmental exposure. Glucagon-like peptide-1 (GLP-1) receptor agonists have markedly improved treatment efficacy, but heterogeneous response, weight recurrence after withdrawal, residual organ risk, and limited long-term pediatric data expose the limitations of a single-pathway, weight-centered model. This narrative review integrates the molecular rationale and clinical evidence for precision pharmacotherapy beyond single GLP-1 receptor agonism. It examines glucose-dependent insulinotropic polypeptide (GIP)/GLP-1 and GLP-1/glucagon co-agonism, triple agonism, amylin combinations, fibroblast growth factor 21 (FGF21) analogues, leptin replacement, and melanocortin-4 receptor (MC4R) agonism, separating established pediatric evidence from adult proof-of-concept and preclinical inference. Genotype-directed treatment for monogenic and syndromic obesity and lesion-directed treatment for acquired hypothalamic obesity provide the clearest examples of causal pathway correction. For common obesity, treatment selection should integrate developmental stage, hyperphagia, insulin resistance, ectopic fat, body composition, genetic context, treatment burden, and early response. Disease modification should be evaluated across growth, puberty, bone and lean tissue, beta cell and hepatic health, physical function, psychosocial well-being, safety, and durability. A dynamic cycle of phenotyping, mechanism-based treatment, multidomain monitoring, adaptation, and longitudinal re-phenotyping may improve the clinical relevance of pediatric obesity care. FAU - Dauleh, Hajar AU - Dauleh H AD - Division of Endocrinology, Department of Pediatric Medicine, Sidra Medicine, Doha PO BOX 26999, Qatar. FAU - Hussain, Khalid AU - Hussain K AUID- ORCID: 0000-0002-5480-7112 AD - Division of Endocrinology, Department of Pediatric Medicine, Sidra Medicine, Doha PO BOX 26999, Qatar. LA - eng PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Glucagon-Like Peptide-1 Receptor Agonists) RN - 0 (Glucagon-Like Peptide-1 Receptor) RN - 0 (Receptor, Melanocortin, Type 4) SB - IM MH - Humans MH - *Glucagon-Like Peptide-1 Receptor Agonists MH - *Precision Medicine/methods MH - *Pediatric Obesity/drug therapy/metabolism MH - Animals MH - Child MH - Glucagon-Like Peptide-1 Receptor/metabolism MH - Receptor, Melanocortin, Type 4 MH - Molecular Targeted Therapy PMC - PMC13705234 OTO - NOTNLM OT - FGF21 OT - GLP-1 receptor agonist OT - amylin OT - disease modification OT - incretin polyagonism OT - melanocortin OT - monogenic obesity OT - pediatric obesity OT - pharmacotherapy OT - precision medicine COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/19 CRDT- 2026/10/27 10:41 PHST- 2026/09/26 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ijms27209269 [pii] AID - ijms-27-09269 [pii] AID - 10.3390/ijms27209269 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 19;27(20):9269. doi: 10.3390/ijms27209269. PMID- 42942034 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 19 TI - Modulation of the Liver-Brain Axis by Ondansetron in Thio-Acetamide-Induced Acute Hepatic Encephalopathy. LID - 10.3390/ijms27209265 [doi] LID - 9265 AB - Hepatic encephalopathy (HE) is a severe neurological complication of liver failure characterized by hyperammonemia, oxidative stress, neuroinflammation, and progressive neuronal dysfunction. Ondansetron (OND), a selective 5-hydroxytryptamine-3 (5-HT(3)) receptor antagonist, has reported anti-inflammatory and neuroactive properties, but its effects in HE remain poorly defined. This study evaluated OND in a thioacetamide (TAA)-induced acute HE model in female Sprague-Dawley rats. Animals were assigned to Control, TAA, OND, TAA+OND (0.5 mg/kg), and TAA+OND (1 mg/kg) groups. Serum and cerebral glutamine, oxidative and antioxidant parameters, histopathological alterations, and TLR2, NF-kappaB, IL-1beta, GFAP, NLRP3, and caspase-1 immunoreactivity were assessed in liver and brain tissues. TAA increased glutamine accumulation, produced tissue injury, altered redox parameters, and increased several inflammation-related markers. OND significantly reduced cerebral glutamine and attenuated selected biochemical, histopathological, and immunohistochemical alterations, with generally more pronounced effects at 0.5 mg/kg. These findings indicate that OND administration is associated with partial modulation of metabolic, oxidative, inflammatory, and structural alterations in TAA-treated rats, warranting further receptor-specific and functional studies to clarify its mechanisms and therapeutic relevance. FAU - Sari, Fatih Mehmet AU - Sari FM AUID- ORCID: 0009-0007-7022-3751 AD - Department of Emergency Medicine, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24100, Turkiye. FAU - Bilgin, Yasin AU - Bilgin Y AUID- ORCID: 0000-0002-1582-223X AD - Department of Emergency Medicine, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24100, Turkiye. FAU - Cicek, Betul AU - Cicek B AUID- ORCID: 0000-0003-1395-1326 AD - Department of Physiology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24100, Turkiye. FAU - Kurt, Engin AU - Kurt E AUID- ORCID: 0000-0001-8044-635X AD - Department of Emergency Medicine, Mengucek Gazi Training and Research Hospital, Erzincan 24100, Turkiye. FAU - Ozkaraca, Mustafa AU - Ozkaraca M AUID- ORCID: 0000-0002-6359-6249 AD - Department of Pathology, Faculty of Veterinary Medicine, Cumhuriyet University, Sivas 58140, Turkiye. FAU - Gungor, Ali AU - Gungor A AUID- ORCID: 0009-0008-7985-0986 AD - Laboratory Technician and Veterinary Health Program, Health Services Vocational School, Osmaniye Korkut Ata University, Osmaniye 80000, Turkiye. FAU - Kurt, Nezahat AU - Kurt N AUID- ORCID: 0000-0002-1685-5332 AD - Department of Biochemistry, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24100, Turkiye. AD - R&D Activities and Research Laboratories, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24100, Turkiye. FAU - Bilgin, Asli Ozbek AU - Bilgin AO AUID- ORCID: 0000-0001-9027-4299 AD - R&D Activities and Research Laboratories, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24100, Turkiye. AD - Department of Pharmacology, Faculty of Medicine, Erzincan Binali Yildirim University, Erzincan 24100, Turkiye. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 075T165X8M (Thioacetamide) RN - 4AF302ESOS (Ondansetron) RN - 0RH81L854J (Glutamine) RN - 0 (Antioxidants) SB - IM MH - Animals MH - *Thioacetamide/toxicity/adverse effects MH - *Ondansetron/pharmacology/therapeutic use MH - *Hepatic Encephalopathy/drug therapy/chemically induced/metabolism/pathology MH - Female MH - *Brain/metabolism/drug effects/pathology MH - Rats MH - *Liver/metabolism/drug effects/pathology MH - Oxidative Stress/drug effects MH - Rats, Sprague-Dawley MH - Glutamine/metabolism MH - Disease Models, Animal MH - Antioxidants/metabolism PMC - PMC13705541 OTO - NOTNLM OT - glutamine metabolism OT - hepatic encephalopathy OT - liver-brain axis OT - neuroinflammation OT - ondansetron OT - oxidative stress COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/19 CRDT- 2026/10/27 10:41 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ijms27209265 [pii] AID - ijms-27-09265 [pii] AID - 10.3390/ijms27209265 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 19;27(20):9265. doi: 10.3390/ijms27209265. PMID- 42942032 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 19 TI - Development and Evaluation of the 40K Liquid SNP Array for Population Genetic Analysis in Golden Pompano (Trachinotus ovatus). LID - 10.3390/ijms27209263 [doi] LID - 9263 AB - Single nucleotide polymorphism (SNP) arrays provide efficient and scalable platforms for genetic diversity assessment, population genomic analysis, and molecular breeding in aquaculture species. However, a high-throughput SNP genotyping platform specifically developed for golden pompano (Trachinotus ovatus), an economically important marine aquaculture fish, remains limited. This study aimed to develop and evaluate a liquid SNP array for T. ovatus and assess its applicability in population genetic analyses. Genome-wide SNP resources were generated from 200 individuals representing 10 cultured populations of T. ovatus. Following SNP discovery, quality filtering, probe evaluation, and marker optimization, a 40K liquid SNP array, designated Changxin-I, was constructed. The performance of Changxin-I was evaluated using an independent validation cohort of 144 individuals from five cultured populations with both WGS-derived and array-derived genotype data available. The array exhibited high genotyping performance, with an average SNP detection rate of 99.94% and a genotype concordance rate of 96.23% with WGS data. Genetic diversity and population structure analyses were performed using Changxin-I genotype data, and the inferred population patterns were compared with those obtained from whole-genome resequencing (WGS) data from the same individuals. A total of 40,476 SNPs were selected for the final Changxin-I array, including 40,442 genome-wide background markers and 34 trait-associated SNPs. The validation results showed high genotyping quality, with an average mapping rate of 98.87% and a mean SNP detection rate of 99.94%. Most SNP markers (99.93%) exhibited minor allele frequencies (MAF) greater than 0.10. Changxin-I successfully revealed genetic diversity and population differentiation among cultured populations, and the population structure patterns were consistent with those obtained from WGS-based analyses. Changxin-I represents a reliable and efficient genomic tool for large-scale SNP genotyping in golden pompano. This array provides a practical platform for population genetic studies, germplasm characterization, and future genomic-assisted breeding applications in T. ovatus. FAU - Liu, Baosuo AU - Liu B AD - Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China. AD - Sanya Tropical Fisheries Research Institute, Sanya 572018, China. AD - Guangdong Engineering Research Center of Key Technologies and Equipment R&D on Modern Marine Ranching, Guangzhou 510300, China. AD - Guangdong Provincial Engineer Technology Research Center of Marine Biological Seed Industry, Guangzhou 510300, China. FAU - Gao, Yayang AU - Gao Y AUID- ORCID: 0009-0005-1255-9578 AD - Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China. FAU - Guo, Huayang AU - Guo H AUID- ORCID: 0000-0003-0883-9065 AD - Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China. AD - Guangdong Engineering Research Center of Key Technologies and Equipment R&D on Modern Marine Ranching, Guangzhou 510300, China. AD - Guangdong Provincial Engineer Technology Research Center of Marine Biological Seed Industry, Guangzhou 510300, China. FAU - Zhu, Kecheng AU - Zhu K AUID- ORCID: 0000-0002-0712-5514 AD - Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China. AD - Guangdong Engineering Research Center of Key Technologies and Equipment R&D on Modern Marine Ranching, Guangzhou 510300, China. AD - Guangdong Provincial Engineer Technology Research Center of Marine Biological Seed Industry, Guangzhou 510300, China. FAU - Xian, Lin AU - Xian L AD - Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China. FAU - Zhang, Nan AU - Zhang N AD - Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China. AD - Guangdong Engineering Research Center of Key Technologies and Equipment R&D on Modern Marine Ranching, Guangzhou 510300, China. FAU - Zhu, Tengfei AU - Zhu T AD - Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China. FAU - Zhang, Dianchang AU - Zhang D AUID- ORCID: 0000-0002-8711-2595 AD - Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China. AD - Sanya Tropical Fisheries Research Institute, Sanya 572018, China. AD - Guangdong Engineering Research Center of Key Technologies and Equipment R&D on Modern Marine Ranching, Guangzhou 510300, China. AD - Guangdong Provincial Engineer Technology Research Center of Marine Biological Seed Industry, Guangzhou 510300, China. LA - eng GR - 2022YFD2400505/Suppoded by the National Key Research and Development Program of China/ GR - Grant No.Guinongke AB241484019/Guangxi Key Research and Development Project/ GR - KJRC2023B15/Innovational Fund for Scientific and Technological Personnel of Hainan Province/ GR - CARS-46/Supported by the earmarked fund for CARS-46/ GR - 2026CXTD27/Innovative Team Building Project of Guangdong Modern Agricultural Industrial Technology System/ GR - 2025-MRB-00-001/2025 Research on breeding technology of candidate species for Guangdong modern marine ranching/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - *Polymorphism, Single Nucleotide MH - Animals MH - *Genetics, Population/methods MH - Genotype MH - *Fishes/genetics MH - Genotyping Techniques/methods MH - *Oligonucleotide Array Sequence Analysis/methods PMC - PMC13705047 OTO - NOTNLM OT - Trachinotus ovatus OT - genetic diversity OT - genomic breeding OT - liquid SNP array OT - population genetics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/19 CRDT- 2026/10/27 10:41 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ijms27209263 [pii] AID - ijms-27-09263 [pii] AID - 10.3390/ijms27209263 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 19;27(20):9263. doi: 10.3390/ijms27209263. PMID- 42942027 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 18 TI - Circulating alpha-Klotho Is Elevated in Breast Cancer and Positively Correlates with IGF-1. LID - 10.3390/ijms27209257 [doi] LID - 9257 AB - Breast cancer (BC) is the most commonly diagnosed cancer among women worldwide. Klotho is an anti-aging protein implicated in tumor suppression; however, the relationship between circulating Klotho levels and BC remains unclear. We measured serum Klotho by enzyme-linked immunosorbent assay (ELISA) in 372 participants: 166 patients with BC, 24 patients with ductal carcinoma in situ (DCIS), and 182 controls, and examined its relation to clinicopathological characteristics, including estrogen receptor (ER), progesterone receptor (PR), HER2 and lymph node status, body mass index (BMI), and insulin-like growth factor 1 (IGF-1). Serum Klotho levels were significantly higher in invasive BC and DCIS patients compared with controls (p = 0.003 and p = 0.001, respectively), whereas no significant difference was detected between invasive BC and DCIS. After adjustment for age, Klotho levels remained significantly higher in BC and DCIS patients than in controls. Klotho levels correlated positively with IGF-1 (p = 0.0043), but not with BMI, ER, PR, HER2, or lymph node status. These preliminary findings demonstrate an association between elevated circulating Klotho and BC, as well as a positive relationship between Klotho and IGF-1. Further studies using age-matched cohorts and diagnostic-performance analyses are required to determine whether circulating Klotho has clinical utility as a BC biomarker. FAU - Ben Neriah, Shira AU - Ben Neriah S AD - Department of Oncology, Tel Aviv Sourasky Medical Center, Tel Aviv 6423906, Israel. FAU - Abboud, Marana AU - Abboud M AD - Department of Oncology, Tel Aviv Sourasky Medical Center, Tel Aviv 6423906, Israel. AD - Faculty of Medical and Health Sciences, Tel Aviv University, Tel Aviv 6997801, Israel. FAU - Berlin, Alla AU - Berlin A AD - Department of Oncology, Tel Aviv Sourasky Medical Center, Tel Aviv 6423906, Israel. FAU - Hemi, Rina AU - Hemi R AD - Endocrinology Institute, Chaim Sheba Medical Center, Tel Hashomer, Ramat Gan 5262000, Israel. FAU - Aks, Rona AU - Aks R AD - Department of Oncology, Tel Aviv Sourasky Medical Center, Tel Aviv 6423906, Israel. FAU - Shapira, Shiran AU - Shapira S AD - Department of Oncology, Tel Aviv Sourasky Medical Center, Tel Aviv 6423906, Israel. AD - Faculty of Medical and Health Sciences, Tel Aviv University, Tel Aviv 6997801, Israel. FAU - Wolf, Ido AU - Wolf I AUID- ORCID: 0000-0001-9319-7612 AD - Department of Oncology, Tel Aviv Sourasky Medical Center, Tel Aviv 6423906, Israel. AD - Faculty of Medical and Health Sciences, Tel Aviv University, Tel Aviv 6997801, Israel. FAU - Rubinek, Tami AU - Rubinek T AUID- ORCID: 0000-0003-4731-8887 AD - Department of Oncology, Tel Aviv Sourasky Medical Center, Tel Aviv 6423906, Israel. AD - Faculty of Medical and Health Sciences, Tel Aviv University, Tel Aviv 6997801, Israel. LA - eng PT - Journal Article DEP - 20261018 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 67763-96-6 (Insulin-Like Growth Factor I) RN - EC 3.2.1.31 (Klotho Proteins) RN - EC 3.2.1.31 (Glucuronidase) RN - EC 3.2.1.31 (KL protein, human) RN - 0 (Biomarkers, Tumor) RN - EC 2.7.10.1 (Erb-b2 Receptor Tyrosine Kinases) RN - 0 (IGF1 protein, human) SB - IM MH - Humans MH - Female MH - *Insulin-Like Growth Factor I/metabolism MH - Klotho Proteins MH - *Breast Neoplasms/blood/pathology/metabolism MH - *Glucuronidase/blood MH - Middle Aged MH - Biomarkers, Tumor/blood MH - Aged MH - Adult MH - Case-Control Studies MH - *Carcinoma, Intraductal, Noninfiltrating/blood/pathology MH - Erb-b2 Receptor Tyrosine Kinases/metabolism PMC - PMC13705368 OTO - NOTNLM OT - IGF-1 OT - Klotho OT - aging OT - biomarker OT - breast cancer OT - tumor suppressor COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/18 CRDT- 2026/10/27 10:41 PHST- 2026/08/11 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - ijms27209257 [pii] AID - ijms-27-09257 [pii] AID - 10.3390/ijms27209257 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 18;27(20):9257. doi: 10.3390/ijms27209257. PMID- 42942026 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 18 TI - Integrative Multi-Omics and Network Pharmacology Analyses Reveal Potential Mechanisms of Codonopsis pilosula in Mountain Sickness. LID - 10.3390/ijms27209256 [doi] LID - 9256 AB - Mountain sickness comprises a spectrum of high-altitude disorders associated with hypobaric hypoxia, including acute mountain sickness (AMS) and high-altitude pulmonary edema (HAPE). Although Codonopsis pilosula has been widely used in traditional medicine for conditions related to hypoxia, its molecular basis in mountain sickness remains poorly understood. Here, we integrated network pharmacology, transcriptomic analysis, machine-learning-based feature selection, single-cell RNA sequencing, and molecular docking to explore potential molecular associations between Codonopsis pilosula-related candidate targets and mountain sickness. We identified a set of Codonopsis pilosula-associated candidate targets enriched in biological processes related to hypoxia response, oxidative stress, inflammation, and vascular regulation. Integrated transcriptomic analyses across high-altitude exposure, AMS, and HAPE revealed convergent alterations in immune-related and oxygen transport-associated programs. Machine-learning analysis further provided internal computational prioritization of candidate genes associated with high-altitude exposure, with recurrent selection across complementary algorithms within the analyzed paired cohort. Single-cell transcriptomic analysis provided additional cellular context for these candidates, revealing distinct expression patterns across peripheral blood and lung cell populations. Molecular docking further identified potential interactions between selected Codonopsis pilosula compounds and candidate proteins, with MMP9, CA2, and BCL2 showing comparatively favorable predicted interactions. Together, these findings provide an exploratory framework for evaluating potential associations between Codonopsis pilosula-associated candidate targets and transcriptional and cellular responses to high-altitude hypoxia, and identify candidate molecular pathways and targets for further experimental investigation. FAU - Zhou, Yaqi AU - Zhou Y AD - State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, College of Life Sciences and Technology, Tarim University, Alar 843300, China. AD - College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. FAU - Wu, Jiamin AU - Wu J AD - State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, College of Life Sciences and Technology, Tarim University, Alar 843300, China. AD - College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. FAU - Tan, Zhiwei AU - Tan Z AD - State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, College of Life Sciences and Technology, Tarim University, Alar 843300, China. FAU - He, Wanxin AU - He W AD - State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, College of Life Sciences and Technology, Tarim University, Alar 843300, China. FAU - Tan, Wen AU - Tan W AD - State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, College of Life Sciences and Technology, Tarim University, Alar 843300, China. FAU - Fu, Mingxue AU - Fu M AD - College of Life Science and Technology, Xinjiang University, Urumqi 830000, China. FAU - Bai, Baowei AU - Bai B AD - State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, College of Life Sciences and Technology, Tarim University, Alar 843300, China. FAU - Li, Hao AU - Li H AUID- ORCID: 0009-0003-8978-1658 AD - State Key Laboratory Incubation Base for Conservation and Utilization of Bio-Resource in Tarim Basin, College of Life Sciences and Technology, Tarim University, Alar 843300, China. AD - College of Biological Sciences, China Agricultural University, Beijing 100193, China. LA - eng GR - BRYB2501/Tarim University/ GR - BTBKXM-2025-Y59/Xinjiang Production and Construction Corps/ PT - Journal Article DEP - 20261018 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Plant Extracts) SB - IM MH - *Altitude Sickness/drug therapy/genetics/metabolism MH - *Codonopsis/chemistry MH - Molecular Docking Simulation MH - Humans MH - *Network Pharmacology/methods MH - Gene Expression Profiling MH - Transcriptome MH - Multiomics MH - *Plant Extracts/pharmacology/chemistry MH - Machine Learning PMC - PMC13705243 OTO - NOTNLM OT - Codonopsis pilosula OT - machine learning OT - molecular docking OT - mountain sickness OT - network pharmacology OT - single-cell RNA sequencing OT - transcriptomics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/18 CRDT- 2026/10/27 10:41 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - ijms27209256 [pii] AID - ijms-27-09256 [pii] AID - 10.3390/ijms27209256 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 18;27(20):9256. doi: 10.3390/ijms27209256. PMID- 42942025 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 18 TI - In Silico and Experimental Evidence for Theasaponin E2 as a Modulator of the AKT1/GSK-3beta Signaling Axis in Osteoclast Differentiation. LID - 10.3390/ijms27209255 [doi] LID - 9255 AB - Theasaponin E2 (TE2) is a triterpenoid saponin isolated from Camellia sinensis seeds with reported anti-inflammatory, antibacterial, and antitumor activities; however, its effects on osteoclasts remain unclear. In this study, TE2 markedly inhibited RANKL-induced osteoclast differentiation in RAW264.7 cells without affecting precursor-cell viability. TE2 also significantly reduced the number and viability of mature, differentiated osteoclasts. Mechanistically, TE2 suppressed RANKL-induced phosphorylation of AKT at Ser473 and GSK-3beta at Ser9, as determined by ELISA. Molecular docking predicted that TE2 could interact with the interdomain allosteric pocket of AKT1 at the pleckstrin homology-kinase domain interface (PDB ID: 7NH5; docking score, -9.8 kcal/mol) and the ATP-binding pocket of GSK-3beta (PDB ID: 5K5N; docking score, -7.7 kcal/mol). The selected docking poses contained multiple hydrogen-bonding interactions with donor-acceptor distances of 2.79-3.19 A. Collectively, these findings indicate that TE2 suppresses osteoclast differentiation and reduces mature osteoclast viability, potentially through modulation of the AKT/GSK-3beta signaling axis. TE2 may therefore represent a promising natural-product candidate for further investigation in conditions characterized by excessive osteoclast-mediated bone resorption. FAU - Zhang, Lifang AU - Zhang L AUID- ORCID: 0009-0005-3727-1618 AD - Osteoimmunology & Drug Discovery Research Group, Department of Biology, College of Science, Mathematics and Technology, Wenzhou-Kean University, 88 Daxue Road, Wenzhou 325060, China. AD - Dorothy and George Hennings College of Science, Mathematics and Technology, Kean University, 1000 Morris Ave, Union, NJ 07083, USA. FAU - Ding, Yili AU - Ding Y AD - Dorothy and George Hennings College of Science, Mathematics and Technology, Kean University, 1000 Morris Ave, Union, NJ 07083, USA. AD - Department of Chemistry, College of Science, Mathematics and Technology, Wenzhou-Kean University, 88 Daxue Road, Wenzhou 325060, China. FAU - Bao, Xinyi AU - Bao X AD - Osteoimmunology & Drug Discovery Research Group, Department of Biology, College of Science, Mathematics and Technology, Wenzhou-Kean University, 88 Daxue Road, Wenzhou 325060, China. AD - Dorothy and George Hennings College of Science, Mathematics and Technology, Kean University, 1000 Morris Ave, Union, NJ 07083, USA. FAU - Deepak, Vishwa AU - Deepak V AUID- ORCID: 0009-0006-0148-255X AD - Osteoimmunology & Drug Discovery Research Group, Department of Biology, College of Science, Mathematics and Technology, Wenzhou-Kean University, 88 Daxue Road, Wenzhou 325060, China. AD - Dorothy and George Hennings College of Science, Mathematics and Technology, Kean University, 1000 Morris Ave, Union, NJ 07083, USA. AD - International Frontier Interdisciplinary Research Institute (IFIRI) of Wenzhou-Kean University, Wenzhou 325060, China. LA - eng GR - ISRG2023032/Wenzhou-Kean University/ GR - KY20250604000450/International Frontier Interdisciplinary Research Institute of Wenzhou-Kean University/ GR - WB20220901000092/Wenzhou-Kean University/ PT - Journal Article DEP - 20261018 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) RN - EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta) RN - 0 (Saponins) RN - 6SMK8R7TGJ (Oleanolic Acid) RN - 0 (RANK Ligand) RN - EC 2.7.11.1 (Akt1 protein, mouse) SB - IM MH - *Osteoclasts/metabolism/drug effects/cytology MH - Animals MH - *Proto-Oncogene Proteins c-akt/metabolism/chemistry MH - *Glycogen Synthase Kinase 3 beta/metabolism/chemistry MH - *Cell Differentiation/drug effects MH - *Signal Transduction/drug effects MH - Molecular Docking Simulation MH - Mice MH - RAW 264.7 Cells MH - *Saponins/pharmacology/chemistry MH - *Oleanolic Acid/analogs & derivatives/pharmacology/chemistry MH - RANK Ligand/metabolism/pharmacology MH - Phosphorylation/drug effects PMC - PMC13705028 OTO - NOTNLM OT - AKT/GSK-3beta signaling OT - RANKL OT - bone resorption OT - molecular docking OT - osteoclastogenesis OT - theasaponin E2 COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/18 CRDT- 2026/10/27 10:41 PHST- 2026/07/18 00:00 [received] PHST- 2026/08/25 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - ijms27209255 [pii] AID - ijms-27-09255 [pii] AID - 10.3390/ijms27209255 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 18;27(20):9255. doi: 10.3390/ijms27209255. PMID- 42942024 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 18 TI - Application of Genotyping-by-Sequencing for Analysis of Breeding Signatures in Russian Spring Barley Collection. LID - 10.3390/ijms27209254 [doi] LID - 9254 AB - The high adaptability of barley (Hordeum vulgare L.) facilitated its cultivation across almost the whole Russian territory and various extreme environments. However, the genetic diversity and population structure of historical Russian barley collections remain poorly characterized at the genomic level. This study provides the first comprehensive genomic description of the barley panel maintained in the GeneAgro seed bank. Using genotyping by sequencing (GBS), we obtained high-quality genotyping data of 158 Russian and 21 foreign accessions. We evaluated genome-wide diversity and population structure, revealing a clear genetic stratification into two-rowed and six-rowed barley. Russian accessions complemented the diversity of the more than 19,000 domesticated barley accessions from the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), expanding the representation of germplasm developed specifically for Russian environments and economic demands. To identify genomic regions that may have been shaped by breeding, we scanned the native GeneAgro barley accessions for selective footprints. We detected multiple such regions across the genome. Some were shared by two-rowed and six-rowed barley, while others were specific to one row type, which may reflect differences in their breeding histories. These regions contain candidate genes previously associated with spike architecture, plant morphology, flowering time, fertility, grain color, photosynthesis, seed dormancy and resistance to fungal pathogens. These candidates provide hypotheses for future allele-level and phenotypic studies. FAU - Afonnikova, Svetlana D AU - Afonnikova SD AD - The Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Novosibirsk Region, Russia. FAU - Pronozin, Artem Yu AU - Pronozin AY AUID- ORCID: 0000-0002-3011-6288 AD - The Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Novosibirsk Region, Russia. FAU - Kiseleva, Antonina A AU - Kiseleva AA AUID- ORCID: 0000-0002-3725-8596 AD - The Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Novosibirsk Region, Russia. FAU - Piskarev, Vjacheslav V AU - Piskarev VV AD - Siberian Research Institute of Plant Cultivation and Breeding-Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Krasnoobsk 630501, Novosibirsk Region, Russia. FAU - Kukoeva, Tatyana V AU - Kukoeva TV AD - The Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Novosibirsk Region, Russia. FAU - Sotnik, Andrey Ya AU - Sotnik AY AD - Siberian Research Institute of Plant Cultivation and Breeding-Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Krasnoobsk 630501, Novosibirsk Region, Russia. FAU - Vasiliev, Gennady V AU - Vasiliev GV AUID- ORCID: 0000-0003-0929-6832 AD - The Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Novosibirsk Region, Russia. FAU - Afonnikov, Dmitry A AU - Afonnikov DA AUID- ORCID: 0000-0001-9738-1409 AD - The Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Novosibirsk Region, Russia. FAU - Salina, Elena A AU - Salina EA AD - The Federal Research Center Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk 630090, Novosibirsk Region, Russia. LA - eng GR - project No. 075-15-2025-580 from 25.06.2025/Ministry of Science and Higher Education of the Russian Federation/ PT - Journal Article DEP - 20261018 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - *Hordeum/genetics MH - Russia MH - *Plant Breeding MH - Genotype MH - Genetic Variation MH - Genome, Plant MH - *Genotyping Techniques/methods MH - Polymorphism, Single Nucleotide PMC - PMC13705539 OTO - NOTNLM OT - Hordeum vulgare OT - barley collections OT - domesticated barley diversity OT - genotyping by sequencing (GBS) OT - traces of breeding COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/18 CRDT- 2026/10/27 10:41 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - ijms27209254 [pii] AID - ijms-27-09254 [pii] AID - 10.3390/ijms27209254 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 18;27(20):9254. doi: 10.3390/ijms27209254. PMID- 42942021 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 18 TI - GLP-1 Receptor Agonist Therapy in Patients with Cancer: A Review of Clinical Outcomes. LID - 10.3390/ijms27209251 [doi] LID - 9251 AB - Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) are increasingly used for the management of obesity and type 2 diabetes, including among patients with cancer. Although previous research has mainly focused on cancer incidence, their associations with clinical outcomes after cancer diagnosis remain less well characterized. Therefore, the present study aimed to evaluate the oncologic and survival outcomes associated with GLP-1 RA use in adults with an established malignancy. We performed a systematic review of comparative studies evaluating GLP-1 RA use in patients with a confirmed cancer diagnosis. PubMed, Scopus and Web of Science were searched for English-language publications from 1 January 2016 to 8 August 2026. Twenty-six retrospective cohort studies were included in the qualitative synthesis. Thirteen studies included selective GLP-1 RAs and tirzepatide within the same exposure group. Study quality was assessed using the Newcastle-Ottawa Scale. Twenty-five studies evaluated overall survival and/or all-cause mortality, while nine assessed cancer recurrence, progression, or related outcomes. Survival and mortality outcomes more often favored the exposed group (especially compared with nonuse) than not, but findings were less consistent in active-comparator analyses. Evidence for recurrence and progression was sparse and heterogeneous, and no clear signal of increased risk was observed. Because all of these were observational studies with substantial differences in populations, comparators, exposure definitions, and duration of follow-up, the survival associations do not imply causality or direct antitumor effects of GLP-1 RAs. Clinically, the preponderance of evidence does not support the use of GLP-1 RAs as cancer-directed therapy, and their use in patients with cancer should be guided by established indications such as obesity and type 2 diabetes. Potential explanations include effects on glycemic and cardiovascular risk factors, treatment selection, residual confounding, or combinations thereof. Prospective studies and randomized controlled trials with cancer-specific outcomes and longer follow-up are needed to further evaluate these associations. FAU - Andronescu, Alexandra Catalina AU - Andronescu AC AUID- ORCID: 0009-0007-9364-3804 AD - 2nd Department, Faculty of Nursing and Health Sciences, "Iuliu Hatieganu" University of Medicine and Pharmacy, 400015 Cluj-Napoca, Romania. FAU - Ciumarnean, Lorena AU - Ciumarnean L AUID- ORCID: 0000-0002-8491-8817 AD - 2nd Department, Faculty of Nursing and Health Sciences, "Iuliu Hatieganu" University of Medicine and Pharmacy, 400015 Cluj-Napoca, Romania. FAU - Orasan, Olga Hilda AU - Orasan OH AUID- ORCID: 0000-0003-4435-142X AD - Department of Internal Medicine, 4th Medical Discipline, "Iuliu Hatieganu" University of Medicine and Pharmacy, 400015 Cluj-Napoca, Romania. FAU - Cozma, Angela AU - Cozma A AD - Department of Internal Medicine, 4th Medical Discipline, "Iuliu Hatieganu" University of Medicine and Pharmacy, 400015 Cluj-Napoca, Romania. FAU - Leah, Nicoleta Valentina AU - Leah NV AD - 2nd Department, Faculty of Nursing and Health Sciences, "Iuliu Hatieganu" University of Medicine and Pharmacy, 400015 Cluj-Napoca, Romania. FAU - Lucaciu, Roxana Liana AU - Lucaciu RL AUID- ORCID: 0000-0003-4920-4760 AD - Department of Pharmaceutical Biochemistry and Clinical Laboratory, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, 400349 Cluj-Napoca, Romania. FAU - Hangan, Adriana Corina AU - Hangan AC AUID- ORCID: 0000-0002-0944-3161 AD - Department of Inorganic Chemistry, Faculty of Pharmacy, "Iuliu Hatieganu" University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. FAU - Procopciuc, Lucia Maria AU - Procopciuc LM AUID- ORCID: 0000-0002-4738-5775 AD - Department of Medical Biochemistry, Faculty of Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, 400349 Cluj-Napoca, Romania. LA - eng PT - Journal Article PT - Review PT - Systematic Review DEP - 20261018 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Glucagon-Like Peptide-1 Receptor) RN - 0 (Glucagon-Like Peptide-1 Receptor Agonists) RN - 0 (Hypoglycemic Agents) RN - OYN3CCI6QE (Tirzepatide) SB - IM MH - Humans MH - Diabetes Mellitus, Type 2/complications/drug therapy MH - Glucagon-Like Peptide-1 Receptor MH - *Glucagon-Like Peptide-1 Receptor Agonists/therapeutic use MH - Hypoglycemic Agents/therapeutic use MH - *Neoplasms/drug therapy/mortality MH - Tirzepatide/therapeutic use MH - Treatment Outcome PMC - PMC13705413 OTO - NOTNLM OT - cancer OT - disease progression OT - glucagon-like peptide-1 receptor agonist OT - mortality OT - overall survival OT - recurrence COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/18 CRDT- 2026/10/27 10:41 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - ijms27209251 [pii] AID - ijms-27-09251 [pii] AID - 10.3390/ijms27209251 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 18;27(20):9251. doi: 10.3390/ijms27209251. PMID- 42942019 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 18 TI - Immunomodulatory Effects of MSC-Derived ExtracellularVesicles in Celiac Disease. LID - 10.3390/ijms27209249 [doi] LID - 9249 AB - Coeliac disease (CeD) is the most frequent chronic enteropathy, in which intestinal lesions are caused by a dysregulated immune response to gluten proteins. We aimed to investigate whether extracellular vesicles (EVs) from mesenchymal stem cells (MSC) exert immunomodulatory effects in experimental models of CeD and are potentially useful to treat CeD. A population of MSCs was obtained from bone marrow blood of adult healthy donors. EVs were purified by ultracentrifugation of MSC-supernatants and characterized in terms of size, protein content and expression of surface markers. The immunomodulatory effects of MSC-EVs were evaluated in vitro on gliadin-specific T-cell lines by detection of interferon (IFN)-gamma production and ex vivo by using duodenal mucosa biopsies by measuring lymphocyte infiltration (CD3 and CD25 positive cells), IFN-gamma and interleukin (IL)-10 secretion. Moreover, the MSC-EVs effects on B-cells in terms of HLA-DQ/DR and CD83/CD86 expression upon antigen stimulation was also evaluated. EVs obtained from non-celiac adult fibroblasts were used as controls. A significant and dose-dependent reduction of IFN-gamma production by T cells in response to gliadin stimulation was observed in the presence of MSC-EVs (p < 0.05), but not with fibroblast-EVs. No modification of the expression of HLA class II molecules and costimulatory CD83 and CD86 markers on B cells upon EV-MSC incubation was found at any tested dose. Moreover, EVs from both MSCs and fibroblasts inhibited the gliadin-induced expansion of CD3+ and CD25+ T cells in organ cultures (p < 0.05), while increasing the IL-10 production (p < 0.05) and reducing IFN-gamma (p = n.s) in organ supernatants. MSC- EVs modulated selected components of the immune response to gliadin in experimental models of CeD, including antigen-induced IFN-gamma production and cellular responses in intestinal organ cultures. The effects observed in organ cultures were not exclusive to MSC-EVs, as EVs from fibroblast also modulated cellular responses. These findings provide a rationale for further preclinical investigation of EV-mediated immunomodulation in CeD. FAU - Ciccocioppo, Rachele AU - Ciccocioppo R AUID- ORCID: 0000-0002-6297-7576 AD - Gastroenterology & Endoscopy Unit, Department of Medicine and Aging Sciences, Ospedale Clinicizzato SS. Annunziata, Gabriele d'Annunzio University, 66100 Chieti, Italy. FAU - Mottola, Ilaria AU - Mottola I AD - Institute of Biochemistry and Cell Biology, CNR, 80131 Naples, Italy. AD - European Laboratory for the Investigation of Food Induced Diseases (ELFID), 80131 Naples, Italy. FAU - Zuliani, Valeria AU - Zuliani V AUID- ORCID: 0000-0003-4485-5820 AD - Haematology Unit, Department of Medicine, A.O.U.I. Policlinico G.B. Rossi, University of Verona, 37134 Verona, Italy. FAU - Adamo, Annalisa AU - Adamo A AD - Haematology Unit, Department of Medicine, A.O.U.I. Policlinico G.B. Rossi, University of Verona, 37134 Verona, Italy. FAU - Vitale, Serena AU - Vitale S AD - Institute of Biochemistry and Cell Biology, CNR, 80131 Naples, Italy. FAU - Krampera, Mauro AU - Krampera M AUID- ORCID: 0000-0002-7280-2040 AD - Hematology and Bone Marrow Transplant Unit, Section of Biomedicine of Innovation, Department of Engineering for Innovative Medicine, University of Verona, 37134 Verona, Italy. FAU - Camarca, Alessandra AU - Camarca A AUID- ORCID: 0000-0001-7546-7527 AD - Institute of Food Sciences, CNR, 83100 Avellino, Italy. FAU - Rotondi Aufiero, Vera AU - Rotondi Aufiero V AD - European Laboratory for the Investigation of Food Induced Diseases (ELFID), 80131 Naples, Italy. AD - Institute of Food Sciences, CNR, 83100 Avellino, Italy. FAU - Pisapia, Laura AU - Pisapia L AD - Institute of Genetics and Biophysics, CNR, 80131 Naples, Italy. FAU - Capuano, Fabiana AU - Capuano F AD - Gastroenterology Unit, San G. Moscati Hospital, 83100 Avellino, Italy. FAU - Mazzarella, Giuseppe AU - Mazzarella G AUID- ORCID: 0000-0002-7294-8277 AD - European Laboratory for the Investigation of Food Induced Diseases (ELFID), 80131 Naples, Italy. AD - Institute of Food Sciences, CNR, 83100 Avellino, Italy. FAU - Del Pozzo, Giovanna AU - Del Pozzo G AUID- ORCID: 0000-0001-8937-9018 AD - Institute of Genetics and Biophysics, CNR, 80131 Naples, Italy. FAU - Gianfrani, Carmen AU - Gianfrani C AD - Institute of Biochemistry and Cell Biology, CNR, 80131 Naples, Italy. AD - European Laboratory for the Investigation of Food Induced Diseases (ELFID), 80131 Naples, Italy. LA - eng GR - RIBa2022 grant/University of Verona/ GR - CNR FOE 2022 DSB.AD006.371 (InvAt-Invecchiamentoattivo e in salute)/National Research Council/ GR - CNR project FOE2021 DBA.AD005.225 (NutrAge)/National Research Council/ PT - Journal Article DEP - 20261018 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 82115-62-6 (Interferon-gamma) RN - 9007-90-3 (Gliadin) RN - 130068-27-8 (Interleukin-10) RN - 0 (Antigens, CD) RN - 0 (Interleukin-2 Receptor alpha Subunit) SB - IM MH - Humans MH - *Celiac Disease/immunology/pathology/therapy/metabolism MH - *Mesenchymal Stem Cells/metabolism/immunology MH - Interferon-gamma/metabolism MH - T-Lymphocytes/immunology/metabolism MH - *Extracellular Vesicles/immunology/metabolism MH - Gliadin/immunology MH - Adult MH - *Immunomodulation MH - Interleukin-10/metabolism/immunology MH - B-Lymphocytes/immunology/metabolism MH - Antigens, CD/metabolism MH - Intestinal Mucosa/immunology/pathology MH - Female MH - Interleukin-2 Receptor alpha Subunit/metabolism MH - Cells, Cultured MH - Fibroblasts/metabolism/immunology PMC - PMC13705273 OTO - NOTNLM OT - coeliac disease OT - extracellular vesicles OT - gluten protein OT - immunomodulation OT - mesenchymal stromal cells OT - mucosal inflammation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/18 CRDT- 2026/10/27 10:41 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - ijms27209249 [pii] AID - ijms-27-09249 [pii] AID - 10.3390/ijms27209249 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 18;27(20):9249. doi: 10.3390/ijms27209249. PMID- 42942018 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 18 TI - ROCK Inhibition Suppresses Thrombospondin-1 and Rewires DNA Methylation in the Trabecular Meshwork. LID - 10.3390/ijms27209248 [doi] LID - 9248 AB - Rho-associated protein kinase (ROCK) inhibitors reduce cytoskeletal tension in trabecular meshwork (TM) cells, but the mechanisms linking this acute response to sustained extracellular matrix remodelling remain incompletely understood. In this study, we investigated whether the ROCK inhibitor Y39983 regulates thrombospondin-1 (THBS1) and DNA methylation programs, elements known to modulate extracellular matrix (ECM) remodelling, in primary human trabecular meshwork (hTM) cells. Primary hTM cells were treated with Y39983, 5-azacytidine (5-Aza, a DNA methyltransferase inhibitor), or both in combination. Gene expression of THBS1, DNA methyltransferase, ECM, and cytoskeletal molecules was assessed using RT-qPCR. THBS1 promoter methylation was examined through methylation-specific PCR, while genome-wide methylation remodelling was profiled using whole-genome bisulphite sequencing (WGBS) followed by pathway enrichment analysis. 5-Azacytidine abolished Y39983-dependent effects on DNMT1 and DNMT3A and attenuated THBS1 regulation. Methylation-specific PCR suggested altered THBS1 promoter methylation after Y39983 treatment, while whole-genome bisulphite sequencing identified 8845 differentially methylated genes, including 5608 hypomethylated and 3237 hypermethylated genes, enriched in ErbB signalling, focal adhesion, and regulation of the actin cytoskeleton. Independently, Y39983 reduced the basal and stimulus-induced expression of THBS1, as well as ECM- and cytoskeleton-associated genes, including COL4A1, COL6A1, and ACTG1. These findings demonstrate that ROCK inhibition is associated with changes in DNMT-related gene expression and DNA methylation patterns in hTM cells. However, because 5-azacytidine has effects beyond selective DNMT inhibition, further studies using orthogonal approaches are required to establish whether DNA methylation directly mediates transcriptional responses to Y-39983. FAU - Shan, Sze-Wan AU - Shan SW AUID- ORCID: 0000-0001-6876-9914 AD - School of Optometry, The Hong Kong Polytechnic University, Hong Kong, China. AD - Centre for Eye and Vision Research (CEVR), 17W Hong Kong Science Park, Hong Kong, China. AD - Research Centre for SHARP Vision (RCSV), The Hong Kong Polytechnic University, Hong Kong, China. FAU - Choy, Kit-Ying AU - Choy KY AUID- ORCID: 0000-0001-8696-4246 AD - School of Optometry, The Hong Kong Polytechnic University, Hong Kong, China. AD - Centre for Eye and Vision Research (CEVR), 17W Hong Kong Science Park, Hong Kong, China. FAU - Ling, Choi-Ying AU - Ling CY AUID- ORCID: 0009-0006-4472-3202 AD - School of Optometry, The Hong Kong Polytechnic University, Hong Kong, China. FAU - Li, Hoi-Lam AU - Li HL AUID- ORCID: 0009-0006-9218-4813 AD - Ophthalmology, Boston University Chobanian & Avedisian School of Medicine, Boston, MA 02118, USA. FAU - Lung, Hong-Lok AU - Lung HL AD - Department of Chemistry, Hong Kong Baptist University, Hong Kong, China. FAU - To, Chi-Ho AU - To CH AD - School of Optometry, The Hong Kong Polytechnic University, Hong Kong, China. AD - Centre for Eye and Vision Research (CEVR), 17W Hong Kong Science Park, Hong Kong, China. AD - Research Centre for SHARP Vision (RCSV), The Hong Kong Polytechnic University, Hong Kong, China. AD - School of Optometry and Vision Sciences, Cardiff University, Cardiff, UK. FAU - Do, Chi-Wai AU - Do CW AUID- ORCID: 0000-0002-7720-7507 AD - School of Optometry, The Hong Kong Polytechnic University, Hong Kong, China. AD - Centre for Eye and Vision Research (CEVR), 17W Hong Kong Science Park, Hong Kong, China. AD - Research Centre for SHARP Vision (RCSV), The Hong Kong Polytechnic University, Hong Kong, China. LA - eng GR - 1-BD6R/The Hong Kong Polytechnic University/ GR - 1-CD65/The Hong Kong Polytechnic University/ GR - Shaffer Grant 2024/Glaucoma Research Foundation/ GR - 09200276/Hong Kong Health and Medical Research Fund/ GR - CEVR/InnoHK initiative of the Innovation and Technology Commission of the Hong Kong Special Administrative Region Government./ PT - Journal Article DEP - 20261018 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Thrombospondin 1) RN - EC 2.7.11.1 (rho-Associated Kinases) RN - M801H13NRU (Azacitidine) RN - 0 (thrombospondin-1, human) RN - EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases) RN - 0 (Pyridines) SB - IM MH - Humans MH - *DNA Methylation/drug effects MH - *Thrombospondin 1/genetics/metabolism MH - *Trabecular Meshwork/metabolism/drug effects/cytology MH - *rho-Associated Kinases/antagonists & inhibitors/metabolism MH - Azacitidine/pharmacology MH - Promoter Regions, Genetic MH - Gene Expression Regulation/drug effects MH - DNA (Cytosine-5-)-Methyltransferases/metabolism/genetics MH - Pyridines/pharmacology MH - Cells, Cultured PMC - PMC13705181 OTO - NOTNLM OT - DNA methylation OT - DNMT1 OT - DNMT3A OT - ROCK inhibitor OT - THBS1 OT - WGBS OT - Y39983 OT - focal adhesion OT - trabecular meshwork COIS- The authors declare no competing interests. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/18 CRDT- 2026/10/27 10:41 PHST- 2026/08/12 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - ijms27209248 [pii] AID - ijms-27-09248 [pii] AID - 10.3390/ijms27209248 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 18;27(20):9248. doi: 10.3390/ijms27209248. PMID- 42942014 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 18 TI - Effects of beta-Glucans on Pro-Inflammatory Markers and Behavioral Phenotype in a Valproic Acid (VPA)-Induced Autism Model in Zebrafish. LID - 10.3390/ijms27209243 [doi] LID - 9243 AB - Autism Spectrum Disorder (ASD) is a multifactorial neurodevelopmental condition in which neuroinflammation, oxidative stress, and synaptic dysfunction act as converging mechanisms. Prenatal exposure to valproic acid (VPA), an antiepileptic drug and histone deacetylase inhibitor, is a recognized environmental risk factor associated with ASD-like phenotypes. Zebrafish, with conserved neuroimmune pathways, rapid development, and quantifiable social behavior, represents a robust model for investigating ASD pathophysiology and testing therapeutic candidates. This study aimed to assess if a previously selected concentration of VPA is capable of inducing persistent alterations from early development through adulthood, and to assess the potential of beta-glucans, natural polysaccharides with antioxidant and immunomodulatory properties, to mitigate VPA-induced alterations. A multimodal approach included morphological evaluation, mitochondrial respiration, quantitative PCR of inflammatory markers, and behavioral assays targeting locomotion, anxiety-like responses, and social preference. Low-dose VPA (25 muM) induced developmental and behavioral difficulties, accompanied by a transient reduction in mitochondrial respiratory capacity. beta-glucan supplementation attenuated selected VPA-induced alterations, including inflammatory gene-expression changes in larvae and behavioral alterations in adulthood. Overall, these findings provide preclinical evidence that beta-glucans can modulate selected VPA-induced inflammatory, molecular and behavioral alterations and support further investigation of their potential effects in neurodevelopmental models. FAU - Camussi, Diletta AU - Camussi D AD - Neurobiology and Molecular Medicine Unit, IRCCS Stella Maris Foundation, 56128 Pisa, Italy. FAU - Marchese, Maria AU - Marchese M AUID- ORCID: 0000-0002-0194-0702 AD - Neurobiology and Molecular Medicine Unit, IRCCS Stella Maris Foundation, 56128 Pisa, Italy. FAU - Ogi, Asahi AU - Ogi A AD - Neurobiology and Molecular Medicine Unit, IRCCS Stella Maris Foundation, 56128 Pisa, Italy. AD - Department of Clinical Sciences and Translational Medicine, University of Rome Tor Vergata, 00133 Rome, Italy. FAU - Fronte, Baldassare AU - Fronte B AUID- ORCID: 0000-0002-8480-318X AD - Department of Veterinary Sciences, University of Pisa, 56126 Pisa, Italy. FAU - Nicoletti, Ferdinando AU - Nicoletti F AD - IRCCS Neuromed, 86077 Pozzilli, Italy. FAU - Santorelli, Filippo Maria AU - Santorelli FM AUID- ORCID: 0000-0002-1359-9062 AD - Neurobiology and Molecular Medicine Unit, IRCCS Stella Maris Foundation, 56128 Pisa, Italy. FAU - Mero, Serena AU - Mero S AUID- ORCID: 0000-0003-2735-6990 AD - Neurobiology and Molecular Medicine Unit, IRCCS Stella Maris Foundation, 56128 Pisa, Italy. LA - eng GR - Ricerca Corrente/Ministero della Salute/ PT - Journal Article DEP - 20261018 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 614OI1Z5WI (Valproic Acid) RN - 0 (beta-Glucans) RN - 0 (Biomarkers) SB - IM MH - Animals MH - *Valproic Acid/adverse effects MH - *beta-Glucans/pharmacology MH - Zebrafish MH - Disease Models, Animal MH - *Behavior, Animal/drug effects MH - Biomarkers/metabolism MH - *Autistic Disorder/chemically induced/drug therapy/metabolism MH - Mitochondria/metabolism/drug effects MH - Female MH - Oxidative Stress/drug effects MH - Phenotype MH - Inflammation/drug therapy/metabolism PMC - PMC13705117 OTO - NOTNLM OT - inflammation OT - oxidative stress OT - valproate (VPA) OT - zebrafish OT - beta-glucan COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/18 CRDT- 2026/10/27 10:41 PHST- 2026/08/21 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - ijms27209243 [pii] AID - ijms-27-09243 [pii] AID - 10.3390/ijms27209243 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 18;27(20):9243. doi: 10.3390/ijms27209243. PMID- 42942008 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 17 TI - PSY Inhibits Colorectal Cancer Growth and Exhibits an In Vitro Synergistic Interaction with 5-Fluorouracil. LID - 10.3390/ijms27209238 [doi] LID - 9238 AB - PSY is a three-herb formula comprising Patriniae Radix, Mori Cortex Radicis, and Coix Seed that was previously shown to inhibit STAT3 signaling in colorectal cancer (CRC) cells. Here, we investigated molecular and metabolic responses to PSY, its antitumor activity in an HCT116 xenograft model, and its interactions with chemotherapy in vitro. Representative chemical markers were qualitatively verified by UPLC-ESI-QTOF MS/MS. Exploratory RNA sequencing with two biological replicates per group highlighted stress-associated and metabolic processes for further investigation. PSY increased BiP, phosphorylated eIF2alpha, ATF4, and CHOP levels, intracellular reactive oxygen species (ROS) levels, and mitochondrial membrane depolarization, while reducing the phosphorylation of PI3K/AKT/mTOR- and ERK-associated proteins. PSY increased the p-AMPK/AMPK ratio and decreased the p-ACC/ACC ratio. PSY reduced several lipid-biosynthetic transcripts and decreased the relative abundance of selected fatty acids in xenograft tumors. Oral administration of PSY suppressed HCT116 xenograft growth without significant body-weight loss. Combination-index (CI) analysis indicated CI-defined in vitro synergistic interactions with 5-fluorouracil (5-FU) at multiple concentration pairs, with interaction patterns varying across HCT116, HT-29, and SW480 cells. Direct cell counting supported the combination response in HCT116 and SW480 cells. The combination increased apoptosis and reduced STAT3 and NF-kappaB p65 phosphorylation relative to single treatments. These findings characterize concurrent, partly discordant signaling and metabolic responses to PSY and support further preclinical evaluation of its in vitro interaction with 5-FU. FAU - Han, Sanghee AU - Han S AUID- ORCID: 0000-0002-4445-5168 AD - Department of Biomedical Science and Technology, Kyung Hee University, Seoul 02447, Republic of Korea. FAU - Kim, Hail AU - Kim H AUID- ORCID: 0009-0009-1520-0308 AD - Department of Biomedical Science and Technology, Kyung Hee University, Seoul 02447, Republic of Korea. FAU - Choi, Hyunjin AU - Choi H AD - Department of Biomedical Science and Technology, Kyung Hee University, Seoul 02447, Republic of Korea. FAU - Oh, Minseo AU - Oh M AUID- ORCID: 0009-0005-3410-585X AD - Department of Biomedical Science and Technology, Kyung Hee University, Seoul 02447, Republic of Korea. FAU - Lee, Min Young AU - Lee MY AD - Korean Medicine Clinical Trial Center, Kyung Hee University Korean Medicine Hospital, Seoul 02454, Republic of Korea. FAU - Lee, Junhee AU - Lee J AUID- ORCID: 0000-0001-6897-2824 AD - College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea. FAU - Ha, In Jin AU - Ha IJ AUID- ORCID: 0000-0002-9210-1401 AD - Korean Medicine Clinical Trial Center, Kyung Hee University Korean Medicine Hospital, Seoul 02454, Republic of Korea. FAU - Ahn, Kwang Seok AU - Ahn KS AUID- ORCID: 0000-0002-2882-0612 AD - College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea. FAU - Lee, Seok-Geun AU - Lee SG AUID- ORCID: 0000-0002-0060-5777 AD - Department of Biomedical Science and Technology, Kyung Hee University, Seoul 02447, Republic of Korea. AD - Korean Medicine Clinical Trial Center, Kyung Hee University Korean Medicine Hospital, Seoul 02454, Republic of Korea. AD - BioNanocomposite Research Center, Kyung Hee University, Seoul 02447, Republic of Korea. LA - eng GR - RS-2021-NR065827/National Research Foundation of Korea/ PT - Journal Article DEP - 20261017 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - U3P01618RT (Fluorouracil) RN - 0 (Reactive Oxygen Species) RN - 0 (STAT3 Transcription Factor) SB - IM MH - Humans MH - *Fluorouracil/pharmacology MH - *Colorectal Neoplasms/drug therapy/metabolism/pathology MH - Animals MH - Drug Synergism MH - Mice MH - HCT116 Cells MH - Xenograft Model Antitumor Assays MH - Cell Proliferation/drug effects MH - Signal Transduction/drug effects MH - Reactive Oxygen Species/metabolism MH - Mice, Nude MH - STAT3 Transcription Factor/metabolism MH - Apoptosis/drug effects MH - Cell Line, Tumor MH - Male PMC - PMC13705401 OTO - NOTNLM OT - 5-fluorouracil OT - PSY OT - colorectal cancer OT - drug combination OT - endoplasmic reticulum stress OT - lipid metabolism COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/17 CRDT- 2026/10/27 10:41 PHST- 2026/08/08 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - ijms27209238 [pii] AID - ijms-27-09238 [pii] AID - 10.3390/ijms27209238 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 17;27(20):9238. doi: 10.3390/ijms27209238. PMID- 42942005 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 17 TI - Hijacking the Message: Insights from Targeting mRNA Export and Translation in AML. LID - 10.3390/ijms27209234 [doi] LID - 9234 AB - Cancer cells hijack RNA metabolism, including splicing, polyadenylation, nuclear export and translation, to change both the amount and the form of the proteins they produce. This creates therapeutic vulnerabilities beyond the mutations and translocations that have guided drug development in acute myeloid leukemia (AML), where outcomes remain poor. Using AML as a paradigm, this review focuses on two nodes of RNA metabolism: the eukaryotic translation initiation factor eIF4E, a cap-chaperone that escorts mRNAs through processing, export and translation, and CRM1/XPO1, the major nuclear exporter of proteins and select RNAs that partners with eIF4E for specific mRNA export activity. We describe the RNA biology dysregulated in patients, the mechanisms of the inhibitors that target these pathways, and their pre-clinical and clinical data across AML and other malignancies. Indeed, early-stage clinical trials in AML and other malignancies show promise, including the use of CRM1/XPO1 inhibitors (selinexor, eltanexor) and eIF4E inhibitors (including ribavirin and tomivosertib). We discuss how selecting patients by eIF4E status, confirming on-target activity, and designing rational combinations could improve next-generation therapies. FAU - Borden, Katherine L B AU - Borden KLB AUID- ORCID: 0000-0003-2188-5074 AD - Department of Pharmacology, Northwestern University, Chicago, IL 60611, USA. AD - Robert H. Lurie Cancer Center, Northwestern University, Chicago, IL 60611, USA. FAU - Guzman, Monica L AU - Guzman ML AD - Division of Hematology/Medical Oncology, Department of Medicine, New York, NY 10021, USA. AD - Sandra & Edward Meyer Cancer Center, Weill Cornell Medicine, New York, NY 10021, USA. AD - Pharmacology Department, Weill Cornell Medicine, New York, NY 10021, USA. LA - eng GR - NCI R01 98571 and 80728/CA/NCI NIH HHS/United States GR - K.L.B.B/Women Helping Others Foundation/ PT - Journal Article PT - Review DEP - 20261017 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (RNA, Messenger) RN - 0 (Eukaryotic Initiation Factor-4E) RN - 0 (Receptors, Cytoplasmic and Nuclear) RN - 0 (Exportin 1 Protein) RN - 0 (Karyopherins) SB - IM MH - Humans MH - *RNA, Messenger/metabolism/genetics MH - *Protein Biosynthesis/drug effects MH - *Leukemia, Myeloid, Acute/metabolism/drug therapy/genetics MH - Eukaryotic Initiation Factor-4E/metabolism/antagonists & inhibitors/genetics MH - Receptors, Cytoplasmic and Nuclear/metabolism/antagonists & inhibitors MH - Exportin 1 Protein MH - Karyopherins/metabolism/antagonists & inhibitors/genetics MH - *RNA Transport/drug effects MH - Animals PMC - PMC13705321 OTO - NOTNLM OT - CRM1/XPO1 OT - clinical trials OT - eIF4E OT - mRNA export OT - mRNA translation COIS- M.L.G. has no conflict of interest. Relevant patents to K.L.B.B.: Combination therapy using ribavirin as an eIF4E inhibitor, Inventors Katherine Borden, Hiba Zahreddine, Biljana Culjkovic-Kraljacic; US8497292B2, GRANTED; Translation dysfunction therapeutics, Inventors Gordon Jamieson, Katherine Borden, Biljana Culjkovic, Alex Kentsis US10342817B2 GRANTED. No royalties received. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/17 CRDT- 2026/10/27 10:41 PHST- 2026/09/19 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - ijms27209234 [pii] AID - ijms-27-09234 [pii] AID - 10.3390/ijms27209234 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 17;27(20):9234. doi: 10.3390/ijms27209234. PMID- 42941996 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 17 TI - Research Progress and Challenges in Targeting GLUT1 in Tumors. LID - 10.3390/ijms27209225 [doi] LID - 9225 AB - The solute carrier family 2 member 1 (SLC2A1), which encodes glucose transporter 1 (GLUT1), has long been recognized as a key driver of aerobic glycolysis in cancer. However, accumulating evidence has substantially expanded this view, revealing that GLUT1 functions not only as a critical mediator of metabolic reprogramming but also as an important regulator linking tumor metabolism to immune evasion. Notably, GLUT1 expression, regulation, and functional dependence are highly context-dependent across tumor types, disease states, and cellular compartments. Recent studies further link GLUT1-dependent glycolytic flux to immune regulation through glucose competition, lactate accumulation, and metabolite-dependent epigenetic programs within the tumor microenvironment. Reflecting this evolving understanding, therapeutic strategies targeting GLUT1 have progressed from simple metabolic inhibition to approaches that exploit tumor-specific metabolic dependencies and extend to regulated cell death, combination therapy, precision delivery, and selective modulation of malignant and non-malignant cellular compartments. These advances support a shift from expression-centered targeting toward strategies guided by functional GLUT1 dependence and cellular context, while recognizing the metabolic and physiological constraints that shape the therapeutic window. In this review, we systematically summarize recent advances in GLUT1 expression heterogeneity, multilayered regulatory networks, metabolic-immune crosstalk, diagnostic and prognostic relevance, and therapeutic targeting, and discuss how these insights may refine biomarker development, patient selection, and the clinical translation of GLUT1-directed precision metabolic therapy. FAU - Chen, Taohua AU - Chen T AD - Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. FAU - Peng, Bi AU - Peng B AD - Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. FAU - Yuan, Xinya AU - Yuan X AD - Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. FAU - Liu, Yizhou AU - Liu Y AD - Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. FAU - Zhang, Jianing AU - Zhang J AD - Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. FAU - Su, Yizhen AU - Su Y AD - Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. FAU - Hu, Guangyuan AU - Hu G AD - Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. FAU - Tao, Zhen AU - Tao Z AD - Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. FAU - Long, Guoxian AU - Long G AD - Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. LA - eng GR - 2025AFD782/Natural Science Foundation of Hubei Province/ GR - 2024ZD0519903/Noncommunicable Chronic Diseases-National Science and Technology Major Project/ PT - Journal Article PT - Review DEP - 20261017 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Glucose Transporter Type 1) RN - 0 (SLC2A1 protein, human) SB - IM MH - Humans MH - *Glucose Transporter Type 1/metabolism/genetics/antagonists & inhibitors MH - *Neoplasms/metabolism/drug therapy/pathology/genetics MH - Metabolic Reprogramming MH - Animals MH - Tumor Microenvironment MH - Gene Expression Regulation, Neoplastic MH - Glycolysis MH - Molecular Targeted Therapy PMC - PMC13705408 OTO - NOTNLM OT - GLUT1 OT - SLC2A1 OT - aerobic glycolysis OT - heterogeneity OT - metabolic-immune crosstalk OT - therapeutic window COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/17 CRDT- 2026/10/27 10:41 PHST- 2026/08/11 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - ijms27209225 [pii] AID - ijms-27-09225 [pii] AID - 10.3390/ijms27209225 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 17;27(20):9225. doi: 10.3390/ijms27209225. PMID- 42941994 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 17 TI - Polyphenols and Flavonoids in microRNA-Regulated Pathways of Doxorubicin-Induced Cardiotoxicity: Mechanistic Evidence, Biomarker Potential and Translational Gaps. LID - 10.3390/ijms27209224 [doi] LID - 9224 AB - Although doxorubicin remains a cornerstone of anticancer therapy, its clinical utility is limited by dose-dependent cardiotoxicity. Current tools for the early detection of doxorubicin-induced cardiotoxicity (DIC) remain suboptimal, prompting interest in microRNAs (miRNAs) as mechanistic regulators and circulating biomarkers. Natural cardioprotective compounds, particularly polyphenols and flavonoids, have also attracted attention because of their ability to modulate oxidative stress, mitochondrial dysfunction, inflammation, autophagy and regulated cell death. However, the mechanistic and translational strength of this evidence varies considerably. This critical narrative review is based on targeted PubMed literature searches up to 14 August 2026, supplemented by selective reference-list screening of relevant original articles and reviews. The literature search was not intended to be exhaustive, and the studies discussed were selected to illustrate different levels of mechanistic evidence. Studies investigating miRNA-mediated mechanisms in DIC were distinguished from circulating biomarker studies, while phytochemical evidence was classified as direct miRNA-linked, miRNA-associated without causal validation, or pathway-convergent. Among the selected examples, taxifolin provides direct miRNA-linked evidence involving the miR-200a/nuclear factor erythroid 2-related factor 2 (NRF2) pathway, with functional miR-200a inhibition demonstrated in H9c2 cells. Blueberry extract provided miRNA-associated evidence; miR-140-5p expression was assessed together with Sirt2 and Nrf2 expression, but a causal role for miR-140-5p was not functionally validated. For the other selected compounds, the available evidence was predominantly pathway-convergent. Although miRNAs and phytochemicals offer complementary perspectives on DIC mechanisms and cardioprotection, their clinical application remains premature. Future studies require functional miRNA validation, standardized biomarker assessment, pharmacokinetic evaluation and confirmation that cardioprotection does not compromise antitumor efficacy. FAU - Lampropoulou, Dimitra Ioanna AU - Lampropoulou DI AUID- ORCID: 0000-0003-3696-8550 AD - Department of Nutritional Science and Dietetics, Faculty of Health Sciences, University of Peloponnese, 24100 Kalamata, Greece. FAU - Kouskouti, Antigoni AU - Kouskouti A AD - Department of Nutritional Science and Dietetics, Faculty of Health Sciences, University of Peloponnese, 24100 Kalamata, Greece. FAU - Gioxari, Aristea AU - Gioxari A AUID- ORCID: 0000-0002-4869-6815 AD - Department of Nutritional Science and Dietetics, Faculty of Health Sciences, University of Peloponnese, 24100 Kalamata, Greece. FAU - Pliakou, Evangelia AU - Pliakou E AUID- ORCID: 0000-0003-0775-1715 AD - Department of Medical Oncology, Athens Naval and Veterans Hospital, 11521 Athens, Greece. FAU - Argyri, Konstantina AU - Argyri K AUID- ORCID: 0009-0000-9162-1889 AD - Department of Nutritional Science and Dietetics, Faculty of Health Sciences, University of Peloponnese, 24100 Kalamata, Greece. FAU - Dedes, Vasileios AU - Dedes V AUID- ORCID: 0000-0003-2555-124X AD - Department of Nutritional Science and Dietetics, Faculty of Health Sciences, University of Peloponnese, 24100 Kalamata, Greece. FAU - Panoutsopoulos, George AU - Panoutsopoulos G AD - Department of Nutritional Science and Dietetics, Faculty of Health Sciences, University of Peloponnese, 24100 Kalamata, Greece. FAU - Gazouli, Maria AU - Gazouli M AUID- ORCID: 0000-0002-3295-6811 AD - Laboratory of Biology, Department of Basic Medical Sciences, Medical School, National and Kapodistrian University of Athens, Michalakopoulou 176, 11527 Athens, Greece. LA - eng PT - Journal Article PT - Review DEP - 20261017 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (MicroRNAs) RN - 80168379AG (Doxorubicin) RN - 0 (Biomarkers) RN - 0 (Flavonoids) RN - 0 (Polyphenols) SB - IM MH - *MicroRNAs/genetics/metabolism MH - Humans MH - *Cardiotoxicity/metabolism/etiology/genetics/drug therapy MH - *Doxorubicin/adverse effects MH - Biomarkers/metabolism MH - *Flavonoids/pharmacology MH - *Polyphenols/pharmacology MH - Animals PMC - PMC13704963 OTO - NOTNLM OT - anthracycline cardiotoxicity OT - biomarker OT - cardio-oncology OT - flavonoids OT - microRNAs OT - polyphenols COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/17 CRDT- 2026/10/27 10:41 PHST- 2026/09/18 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - ijms27209224 [pii] AID - ijms-27-09224 [pii] AID - 10.3390/ijms27209224 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 17;27(20):9224. doi: 10.3390/ijms27209224. PMID- 42941986 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 17 TI - Acetylcholine and Prolactin, Other Factors in Breast Cancer. LID - 10.3390/ijms27209216 [doi] LID - 9216 AB - Acetylcholine (ACh) and prolactin (PRL) are increasingly recognized as additional factors potentially influencing breast cancer biology. Their effects may depend on tumor-cell context and on the molecular signaling state of individual breast cancer subtypes. This study aimed to evaluate phosphorylation responses to ACh and PRL and examine transcriptomic associations involving related signaling genes. MCF7, T47D, and MDA-MB-231 breast cancer cell lines were treated with ACh, PRL, or combined ACh + PRL for 4 h. Significant changes in selected phosphoproteins were summarized for proteins related to AKT-S6 kinase, EGFR-ERK, TP53-Src/PLC-gamma1, and STAT3-JUN signaling. Transcriptomic analyses evaluated associations between acetylcholinesterase (ACHE) or prolactin receptor (PRLR) expression and selected signaling-related genes in breast cancer cohorts, including subtype-specific analyses, tumor-versus-normal comparisons, and overall-survival associations. ACh and PRL produced distinct, cell-line-specific phosphorylation patterns. The MCF7 cell line showed increased AKT, p70S6K, and p53 phosphorylation after ACh, whereas combined treatment increased AKT and STAT3 phosphorylation. The T47D cell line showed a mixed response, with combined treatment increasing p70S6K, PLC-gamma1, and Src phosphorylation but reducing AKT, ERK1/2, and c-Jun phosphorylation. The MDA-MB-231 cell line showed the broadest response, with combined ACh + PRL increasing AKT, RSK, STAT3, and c-Jun phosphorylation. Transcriptomic analyses identified subtype-dependent associations of ACHE and PRLR with genes encoding AKT, S6 kinase, ERK, Src/PLC-gamma1, STAT3, and JUN-related proteins. Several genes also showed differential tumor-versus-normal expression or survival associations. ACh and PRL are associated with context-dependent signaling responses in breast cancer. Experimental phosphoprotein changes and tumor transcriptomic associations converged on related signaling modules but represented different molecular levels and did not establish complete pathway activation or direct causality. These findings support further investigation of ACh-PRL interactions and their potential contribution to breast cancer biology. FAU - Calaf, Gloria M AU - Calaf GM AUID- ORCID: 0000-0003-3542-296X AD - Instituto de Alta Investigacion, Universidad de Tarapaca, Arica 1000000, Chile. FAU - Andrade-Madrigal, Cristian AU - Andrade-Madrigal C AD - Laboratorio de Oncovirologia, Departamento de Ciencias Biomedicas, Facultad de Medicina, Universidad de Tarapaca, Arica 1000000, Chile. FAU - Crispin, Leodan A AU - Crispin LA AUID- ORCID: 0000-0002-4026-2641 AD - Instituto de Alta Investigacion, Universidad de Tarapaca, Arica 1000000, Chile. FAU - Miranda, Victor H AU - Miranda VH AD - Instituto de Alta Investigacion, Universidad de Tarapaca, Arica 1000000, Chile. FAU - Aguayo, Francisco AU - Aguayo F AUID- ORCID: 0000-0002-9619-7535 AD - Laboratorio de Oncovirologia, Departamento de Ciencias Biomedicas, Facultad de Medicina, Universidad de Tarapaca, Arica 1000000, Chile. LA - eng GR - FONDECYT #1231537 (G.M.C.)/Fondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT/ANID)/ GR - FONDECYT #1261779 (F.A.)/Fondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT/ANID)/ GR - ANID Ph.D. scholarship #21201758 (C.A-M.)/Agencia Nacional de Investigacion y Desarrollo (ANID)/ PT - Journal Article DEP - 20261017 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 9002-62-4 (Prolactin) RN - 0 (STAT3 Transcription Factor) RN - 0 (Tumor Suppressor Protein p53) RN - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt) RN - 0 (Receptors, Prolactin) SB - IM MH - Humans MH - *Breast Neoplasms/metabolism/genetics/pathology MH - *Prolactin/pharmacology/metabolism MH - Female MH - Phosphorylation/drug effects MH - Signal Transduction/drug effects MH - Cell Line, Tumor MH - MDA-MB-231 Cells MH - STAT3 Transcription Factor/metabolism MH - Gene Expression Regulation, Neoplastic/drug effects MH - MCF-7 Cells MH - Tumor Suppressor Protein p53/metabolism MH - Proto-Oncogene Proteins c-akt/metabolism MH - Receptors, Prolactin/metabolism/genetics PMC - PMC13705242 OTO - NOTNLM OT - ACHE OT - AKT OT - ERK OT - PRLR OT - STAT3 OT - acetylcholine OT - breast cancer OT - kinase arrays OT - prolactin COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analysis, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/17 CRDT- 2026/10/27 10:41 PHST- 2026/08/07 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - ijms27209216 [pii] AID - ijms-27-09216 [pii] AID - 10.3390/ijms27209216 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 17;27(20):9216. doi: 10.3390/ijms27209216. PMID- 42941981 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 16 TI - Atopic Dermatitis in the Era of Exposome and Artificial Intelligence. LID - 10.3390/ijms27209209 [doi] LID - 9209 AB - Atopic dermatitis (AD) is a heterogeneous, relapsing inflammatory skin disease in which epidermal barrier dysfunction, immune dysregulation, microbial imbalance, genetics, and the environment interact continuously over time. Although a substantial body of literature has linked AD with air pollution, weather, allergens, stress, infections, sleep disturbance, skin care practices and microbial perturbation, most studies have assessed single exposures in isolation. This reductionist approach only partially reflects the real-world biology of AD, where patients experience dynamic combinations of external and internal exposures whose effects are non-linear, cumulative and modified by age, phenotype, treatment and susceptibility. The exposome paradigm offers a broader conceptual lens by considering the totality of exposures over the life course. However, exposomic research in AD is methodologically challenging because the data are multimodal, high-dimensional, time-dependent, and often incomplete. Artificial intelligence (AI) can help address this complexity by integrating heterogeneous data layers, modelling temporal dependencies, discovering latent patterns, and generating individualized predictions. This narrative review examines current evidence and proposes a theoretical framework that integrates AI and the exposome in AD. We define the principal domains of the AD exposome, describe a multilevel analytical architecture spanning environmental, clinical, behavioural, microbiome, and multi-omics data. We distinguish applications evaluated in AD studies from exploratory analyses and proposed objectives: prediction of annual flare burden remains exploratory, whereas exposome-informed short-term flare alerts are proposed applications. Although selected AI applications have been evaluated in AD, the added predictive value of exposure data and the clinical utility of a fully integrated exposome-AI system remain to be established. We also outline key methodological and translational priorities, including exposure assessment, temporality, treatment-confounding, interpretability, fairness, data governance, and external validation. The proposed framework offers a structured basis for future research, with clinical translation dependent on prospective validation and evidence of benefit to patient care. FAU - Manti, Sara AU - Manti S AUID- ORCID: 0000-0001-5420-8411 AD - Pediatric Unit, Department of Human Pathology in Adult and Developmental Age "Gaetano Barresi", University of Messina, 98125 Messina, Italy. FAU - Li Pomi, Federica AU - Li Pomi F AUID- ORCID: 0000-0001-6760-9468 AD - Department of Precision Medicine in Medical, Surgical and Critical Care (Me.Pre.C.C.), University of Palermo, 90127 Palermo, Italy. FAU - Gammeri, Luca AU - Gammeri L AUID- ORCID: 0000-0001-7524-0103 AD - Department of Biomedical and Dental Science and Morphofunctional Imaging, University of Messina, 98125 Messina, Italy. FAU - Borgia, Francesco AU - Borgia F AUID- ORCID: 0000-0003-3515-8441 AD - Section of Dermatology, Department of Clinical and Experimental Medicine, University of Messina, 98125 Messina, Italy. FAU - Murdaca, Giuseppe AU - Murdaca G AUID- ORCID: 0000-0002-6403-6905 AD - Department of Internal Medicine, University of Genova, 16132 Genova, Italy. AD - Allergology and Clinical Immunology Unit, San Bartolomeo Hospital, 19038 Sarzana, Italy. FAU - Gangemi, Sebastiano AU - Gangemi S AD - Allergy and Clinical Immunology Unit, Department of Clinical and Experimental Medicine, University of Messina, 98125 Messina, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - Humans MH - *Dermatitis, Atopic/etiology/epidemiology MH - *Artificial Intelligence MH - *Exposome MH - Environmental Exposure/adverse effects PMC - PMC13705081 OTO - NOTNLM OT - air pollution OT - artificial intelligence OT - atopic dermatitis OT - biomarkers OT - digital health OT - eczema OT - exposome OT - flare prediction OT - machine learning OT - microbiome OT - precision medicine COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/16 CRDT- 2026/10/27 10:41 PHST- 2026/09/20 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ijms27209209 [pii] AID - ijms-27-09209 [pii] AID - 10.3390/ijms27209209 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 16;27(20):9209. doi: 10.3390/ijms27209209. PMID- 42941974 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 16 TI - QSAR-Guided In Silico Design of Piperazinylanthranilamide Factor Xa Inhibitors: Applicability-Domain Assessment, ADMET Profiling, Molecular Docking, Molecular Dynamics, and MM/GBSA. LID - 10.3390/ijms27209204 [doi] LID - 9204 AB - An integrated computational workflow was developed to prioritize piperazinylanthranilamide factor Xa (FXa) inhibitor candidates. Twenty literature compounds were geometry-optimized and described using alvaDesc to construct a constrained additive multivariate adaptive regression splines model. Internal validation and an assessment of the applicability domain guided the evaluation of 20 prospective analogs. Ten selected analogs and two same-series references were docked to FXa, with apixaban as the crystallographic comparator. Thirteen initial 100 ns molecular dynamics simulations were supplemented by two independent runs for each of PAX-M09, PAX-M15, and PAX-M18. Binding energetics were assessed using molecular mechanics/generalized Born surface area (MM/GBSA), alongside in silico developability profiling. The model achieved R(2) = 0.904 and an internal leave-one-out Q(2) of 0.846. PAX-M09 was the strongest strict in-domain prediction, whereas PAX-M15 was borderline. Apixaban redocking yielded a root-mean-square deviation (RMSD) of 0.953 A. Although PAX-M17 and PAX-M19 ranked highest in docking, subsequent analyses prioritized PAX-M15, PAX-M09, and PAX-M18. PAX-M15 showed the most consistent positional behavior across replicates and consistently favorable run-specific MM/GBSA estimates (-68.76, -70.11, and -69.42 kcal/mol). PAX-M09 and PAX-M18 showed greater between-run heterogeneity. Solubility, metabolic, and toxicity liabilities remained important. The results support experimental investigation of the three candidates without changing their applicability-domain classifications or establishing superior experimental potency. FAU - Gordon, Pawel AU - Gordon P AUID- ORCID: 0009-0007-7926-8753 AD - University of Health Sciences in Bydgoszcz, Jagiellonska 4 Str., 85-067 Bydgoszcz, Poland. FAU - Janiak, Michal AU - Janiak M AD - Department of Toxicology and Bromatology, Faculty of Pharmacy, L. Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, A. Jurasza 2 Street, 85-089 Bydgoszcz, Poland. AD - Doctoral School of Medical and Health Sciences, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University in Torun, Jagiellonska 13-15 Str., 85-067 Bydgoszcz, Poland. FAU - Gackowski, Marcin AU - Gackowski M AUID- ORCID: 0000-0001-9176-7108 AD - Department of Toxicology and Bromatology, Faculty of Pharmacy, L. Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, A. Jurasza 2 Street, 85-089 Bydgoszcz, Poland. FAU - Zielinska-Pisklak, Monika AU - Zielinska-Pisklak M AUID- ORCID: 0000-0002-4457-6381 AD - Department of Pharmaceutical Chemistry and Biomaterials, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha Str., 02-093 Warsaw, Poland. FAU - Szeleszczuk, Lukasz AU - Szeleszczuk L AUID- ORCID: 0000-0002-5302-3797 AD - Department of Organic and Physical Chemistry, Medical University of Warsaw, 1 Banacha Str., 02-097 Warsaw, Poland. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Factor Xa Inhibitors) RN - 0 (ortho-Aminobenzoates) RN - EC 3.4.21.6 (Factor Xa) RN - 0 (Piperazines) SB - IM MH - *Quantitative Structure-Activity Relationship MH - *Molecular Docking Simulation MH - Molecular Dynamics Simulation MH - *Factor Xa Inhibitors/chemistry/pharmacology MH - *ortho-Aminobenzoates/chemistry/pharmacology MH - *Drug Design MH - *Factor Xa/chemistry/metabolism MH - Humans MH - Protein Binding MH - *Piperazines/chemistry/pharmacology MH - Binding Sites PMC - PMC13705391 OTO - NOTNLM OT - ADMET OT - MARSplines OT - MM/GBSA OT - QSAR OT - applicability domain OT - factor Xa OT - molecular docking OT - molecular dynamics OT - piperazinylanthranilamides COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/16 CRDT- 2026/10/27 10:41 PHST- 2026/09/28 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ijms27209204 [pii] AID - ijms-27-09204 [pii] AID - 10.3390/ijms27209204 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 16;27(20):9204. doi: 10.3390/ijms27209204. PMID- 42941972 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 16 TI - Identification of Calcium-Sensing Receptor (CAS) as a Phytaspase Interactor: Implications for the CAS Trafficking Pathway. LID - 10.3390/ijms27209202 [doi] LID - 9202 AB - Proteolytic enzymes play important and diverse roles in regulating protein homeostasis in living cells. Phytaspase, a member of the plant subtilisin-like protease family possessing a 'caspase-like' selectivity and specificity of hydrolysis of its targets, is a cell death-related protease. Phytaspase displays a peculiar trafficking pattern and is involved in the execution of plant cell death induced by biotic and abiotic stresses. To elucidate the molecular mechanisms of phytaspase functioning, a search for Nicotiana tabacum phytaspase (NtPhyt)-interacting proteins was performed using the BioID approach. This search identified the calcium-sensing receptor (CAS) protein as a candidate NtPhyt interactor. Direct interaction of NtPhyt with N. tabacum CAS (NtCAS) was corroborated by an in vitro analysis utilizing the purified proteins. Furthermore, NtPhyt was shown to introduce five cleavages in the CAS molecule in vitro. For NtCAS-EGFP produced in N. benthamiana leaves, dual localization to chloroplasts and the cell periphery, including the plasma membrane, was observed, whereas NtPhyt proteolytic activity was undetectable in the chloroplasts. A CAS precursor protein apparently preserving the chloroplast transit peptide was found to accumulate upon a light-to-dark transition and to disappear in the presence of elevated levels of NtPhyt. A requirement for CAS for stress-induced retrograde transport of NtPhyt was uncovered, and partial co-localization of CAS with NtPhyt was observed in cells subjected to oxidative stress. Our results are consistent with the model according to which CAS transiently localizes to the plasma membrane as an intermediate location on its way to its final destination, the chloroplasts, and interacts with NtPhyt at this stage. FAU - Litvinova, Anastasia V AU - Litvinova AV AUID- ORCID: 0009-0006-9664-2973 AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119991, Russia. AD - Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow 119991, Russia. FAU - Pigidanov, Artemii A AU - Pigidanov AA AUID- ORCID: 0009-0006-3888-5707 AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119991, Russia. AD - Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow 119991, Russia. FAU - Torosian, Tatevik A AU - Torosian TA AD - Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow 119991, Russia. FAU - Zgoda, Victor G AU - Zgoda VG AUID- ORCID: 0000-0002-4532-4274 AD - Institute of Biomedical Chemistry, Moscow 119121, Russia. FAU - Serebryakova, Marina V AU - Serebryakova MV AUID- ORCID: 0000-0002-5402-8215 AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119991, Russia. FAU - Kovaleva, Irina E AU - Kovaleva IE AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119991, Russia. FAU - Mamedov, Mahir D AU - Mamedov MD AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119991, Russia. FAU - Golyshev, Sergei A AU - Golyshev SA AUID- ORCID: 0000-0002-4877-1061 AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119991, Russia. FAU - Safronov, Grigoriy G AU - Safronov GG AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119991, Russia. AD - Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow 119991, Russia. FAU - Chichkova, Nina V AU - Chichkova NV AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119991, Russia. FAU - Vartapetian, Andrey B AU - Vartapetian AB AUID- ORCID: 0000-0003-2296-0929 AD - Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119991, Russia. LA - eng GR - 22-14-00071-P/Russian Science Foundation/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Plant Proteins) RN - 0 (Receptors, Calcium-Sensing) RN - EC 3.4.22.- (Caspases) SB - IM MH - *Nicotiana/metabolism/genetics MH - Protein Transport MH - *Plant Proteins/metabolism/genetics MH - *Receptors, Calcium-Sensing/metabolism/genetics MH - Chloroplasts/metabolism MH - *Caspases/metabolism MH - Protein Binding PMC - PMC13705120 OTO - NOTNLM OT - calcium-sensing receptor OT - chloroplast OT - phytaspase OT - plant cell death OT - plasma membrane OT - protein trafficking OT - proteolysis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/16 CRDT- 2026/10/27 10:41 PHST- 2026/09/13 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ijms27209202 [pii] AID - ijms-27-09202 [pii] AID - 10.3390/ijms27209202 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 16;27(20):9202. doi: 10.3390/ijms27209202. PMID- 42941970 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 16 TI - HDL Functionality, Cholesterol-Related Biomarkers and Incidence of Dementia: A Case-Cohort Study in Older Adults at High Cardiovascular Risk. LID - 10.3390/ijms27209197 [doi] LID - 9197 AB - Associations of lipid profiles, including qualitative and functional high-density lipoprotein (HDL) traits, and cholesterol-related biomarkers with incident dementia and Alzheimer's disease (AD), remain unclear. In this regard, older adults at high cardiovascular risk are at particularly high risk of developing dementia. The aim is to evaluate whether these biomarkers are associated with the incidence of dementia and AD in older adults at high cardiovascular (CV) risk. In a case-cohort study, we selected 106 incident dementia cases (82 AD) and a random subcohort of 201 non-cases. We quantified (baseline and at one-year), lipid profile; HDL-mediate cholesterol efflux capacity in a model of human neurons; HDL-mediated anti-inflammatory protection in a model of brain endothelial cells; 24S-hydroxycholesterol and apolipoprotein E. Multivariable-adjusted Cox models were addressed. HDL cholesterol was inversely associated, whereas triglycerides directly associated, with dementia and AD incidence. Higher HDL-mediated cholesterol efflux was inversely associated with dementia (HR 0.64, 95% confidence interval (CI) 0.45-0.91) and AD (HR 0.52, 0.35-0.76). Greater HDL-mediated endothelial protection was associated with lower dementia and AD incidence (HR 0.68, 95% CI 0.47 to 0.99 and HR 0.61, 95% CI 0.41 to 0.91, respectively). A one-year increase in 24S-hydroxycholesterol was associated with a higher incidence of any dementia (HR 2.38, 1.33-4.27) and AD (HR 2.24, 1.11-4.53). Enhanced HDL functional properties ex vivo, in preclinical models using human brain cells, together with lower levels of 24S-hydroxycholesterol, are inversely associated with the incidence of dementia and AD, highlighting these factors as promising prognostic targets in older adults at high CV risk. FAU - Malcampo, Mireia AU - Malcampo M AUID- ORCID: 0000-0002-1916-4537 AD - Hospital del Mar Research Institute (IMIM), 08003 Barcelona, Spain. FAU - Hernaez, Alvaro AU - Hernaez A AD - Hospital del Mar Research Institute (IMIM), 08003 Barcelona, Spain. AD - Blanquerna School of Health Sciences, University Ramon Llull, 08025 Barcelona, Spain. AD - Consorcio CIBER, M.P. Enfermedades Cardiovasculares (CIBERCV), Instituto de Salud Carlos III, 28029 Madrid, Spain. FAU - Hernando-Redondo, Javier AU - Hernando-Redondo J AUID- ORCID: 0000-0002-6777-1649 AD - Hospital del Mar Research Institute (IMIM), 08003 Barcelona, Spain. FAU - Ros, Emilio AU - Ros E AUID- ORCID: 0000-0002-2573-1294 AD - August Pi i Sunyer Biomedical Research Institute (IDIBAPS), 08036 Barcelona, Spain. AD - CIBER, M.P. Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain. FAU - Corella, Dolores AU - Corella D AUID- ORCID: 0000-0002-2366-4104 AD - CIBER, M.P. Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain. AD - Department of Preventive Medicine, University of Valencia, 46010 Valencia, Spain. FAU - Martinez-Gonzalez, Miguel Angel AU - Martinez-Gonzalez MA AUID- ORCID: 0000-0002-3917-9808 AD - CIBER, M.P. Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain. AD - Department of Preventive Medicine and Public Health, Universidad de Navarra, 31008 Pamplona, Spain. AD - Instituto de Investigacion Sanitaria de Navarra (IdiSNA), 31008 Pamplona, Spain. AD - Department of Nutrition, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA. FAU - Salas-Salvado, Jordi AU - Salas-Salvado J AUID- ORCID: 0000-0003-2700-7459 AD - CIBER, M.P. Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain. AD - Unitat de Alimentacio, Nutricio, Desenvolupament i Salut Mental, Departament de Bioquimica i Biotecnologia, Universitat Rovira i Virgili, 43201 Reus, Spain. AD - Institut d'Investigacio Sanitaria Pere Virgili (IISPV), 43204 Reus, Spain. FAU - Estruch, Ramon AU - Estruch R AUID- ORCID: 0000-0003-1260-4445 AD - August Pi i Sunyer Biomedical Research Institute (IDIBAPS), 08036 Barcelona, Spain. AD - CIBER, M.P. Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain. AD - Internal Medicine Service, Hospital Clinic, 08036 Barcelona, Spain. FAU - Timiraos, Juan AU - Timiraos J AD - CIBER, M.P. Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain. AD - Department of Cardiology, Hospital Universitario de Alava, 01004 Vitoria, Spain. FAU - Gomez-Gracia, Enrique AU - Gomez-Gracia E AD - Department of Preventive Medicine, University of Malaga, 29071 Malaga, Spain. FAU - Fiol, Miquel AU - Fiol M AD - CIBER, M.P. Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain. AD - Research Group on Nutritional Epidemiology & Cardiovascular Physiopathology, Health Research Institute of the Balearic Islands, University Hospital Son Espases, 07120 Palma de Mallorca, Spain. FAU - Lapetra, Jose AU - Lapetra J AUID- ORCID: 0000-0002-3873-5737 AD - CIBER, M.P. Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain. AD - Research Unit, Department of Family Medicine, Distrito Sanitario Atencion Primaria Sevilla, 41013 Sevilla, Spain. FAU - Serra-Majem, Lluis AU - Serra-Majem L AUID- ORCID: 0000-0002-9658-9061 AD - CIBER, M.P. Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain. AD - Research Group in Nutritional Epidemiology and Cardiovascular Pathophysiology, Instituto de Investigacion Sanitaria Illes Balears (IdISBa), 35016 Las Palmas de Gran Canarias, Spain. AD - Servicio Canario de Salud, Centro Hospitalario Universitario Insular Materno Infantil, 35016 Las Palmas de Gran Canarias, Spain. FAU - Riera-Mestre, Antoni AU - Riera-Mestre A AUID- ORCID: 0000-0001-9411-804X AD - CIBER, M.P. Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain. AD - Lipids and Vascular Risk Unit, Internal Medicine, Hospital Universitario de Bellvitge Institut d'Investigacio Biomedica de Bellvitge, 08907 Hospitalet de Llobregat, Spain. AD - Faculty of Medicine and Health Sciences, Universitat de Barcelona, 08036 Barcelona, Spain. FAU - Pinto, Xavier AU - Pinto X AUID- ORCID: 0000-0002-2216-2444 AD - CIBER, M.P. Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain. AD - Lipids and Vascular Risk Unit, Internal Medicine, Hospital Universitario de Bellvitge Institut d'Investigacio Biomedica de Bellvitge, 08907 Hospitalet de Llobregat, Spain. FAU - Puig-Pijoan, Albert AU - Puig-Pijoan A AD - Hospital del Mar Research Institute (IMIM), 08003 Barcelona, Spain. AD - Cognitive and Behavioural Neurology Unit, Department of Neurology, Hospital del Mar, 08003 Barcelona, Spain. FAU - Ruiz-Canela, Miguel AU - Ruiz-Canela M AUID- ORCID: 0000-0002-7684-2787 AD - CIBER, M.P. Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain. AD - Department of Preventive Medicine and Public Health, Universidad de Navarra, 31008 Pamplona, Spain. AD - Instituto de Investigacion Sanitaria de Navarra (IdiSNA), 31008 Pamplona, Spain. FAU - Sorli, Jose V AU - Sorli JV AUID- ORCID: 0000-0002-0130-2006 AD - CIBER, M.P. Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain. AD - Department of Preventive Medicine, University of Valencia, 46010 Valencia, Spain. FAU - Babio, Nancy AU - Babio N AUID- ORCID: 0000-0003-3527-5277 AD - CIBER, M.P. Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain. AD - Unitat de Alimentacio, Nutricio, Desenvolupament i Salut Mental, Departament de Bioquimica i Biotecnologia, Universitat Rovira i Virgili, 43201 Reus, Spain. AD - Institut d'Investigacio Sanitaria Pere Virgili (IISPV), 43204 Reus, Spain. FAU - Lamuela-Raventos, Rosa Maria AU - Lamuela-Raventos RM AUID- ORCID: 0000-0002-1287-4560 AD - CIBER, M.P. Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain. AD - Polyphenol Research Group, Department of Nutrition, Food Science and Gastronomy, Faculty of Pharmacy and Food Sciences, INSA-UB, University of Barcelona, 08921 Barcelona, Spain. FAU - Khymenets, Olha AU - Khymenets O AD - Hospital del Mar Research Institute (IMIM), 08003 Barcelona, Spain. AD - CIBER, M.P. Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain. FAU - Sala-Vila, Aleix AU - Sala-Vila A AD - Hospital del Mar Research Institute (IMIM), 08003 Barcelona, Spain. AD - CIBER, M.P. Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain. FAU - Castaner, Olga AU - Castaner O AD - Hospital del Mar Research Institute (IMIM), 08003 Barcelona, Spain. AD - CIBER, M.P. Epidemiologia y Salud Publica (CIBERESP), Instituto de Salud Carlos III, 28029 Madrid, Spain. AD - Primary Care Research Unit Metropolitana Sud, Institut Catala de la Salut, 08820 El Prat de Llobregat, Spain. AD - Institut Universitari de Recerca en Atencio Primaria Jordi Gol i Gurina, 08007 Barcelona, Spain. FAU - Fito, Montserrat AU - Fito M AD - Hospital del Mar Research Institute (IMIM), 08003 Barcelona, Spain. AD - CIBER, M.P. Fisiopatologia de la Obesidad y Nutricion (CIBEROBN), Instituto de Salud Carlos III, 28029 Madrid, Spain. LA - eng GR - PI25/00015, PI17/00214, PI20/00012, PI24/00326/Instituto de Salud Carlos III/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Biomarkers) RN - 0 (Cholesterol, HDL) RN - 97C5T2UQ7J (Cholesterol) RN - 0 (Hydroxycholesterols) RN - 0 (Apolipoproteins E) RN - 47IMW63S3F (24-hydroxycholesterol) SB - IM MH - Humans MH - Male MH - *Biomarkers/blood/metabolism MH - Female MH - Aged MH - *Dementia/epidemiology/blood/metabolism/etiology MH - Incidence MH - *Cholesterol, HDL/blood/metabolism MH - *Cholesterol/blood/metabolism MH - *Cardiovascular Diseases/blood/epidemiology MH - Alzheimer Disease/epidemiology/blood MH - Risk Factors MH - Aged, 80 and over MH - Case-Control Studies MH - Cohort Studies MH - Hydroxycholesterols/blood MH - Apolipoproteins E/blood PMC - PMC13705062 OTO - NOTNLM OT - Alzheimer's disease OT - cardiovascular OT - cholesterol efflux capacity OT - dementia OT - high-density lipoprotein OT - lipid OT - neurodegenerative disease COIS- E.R. reports personal fees, grants, and nonfinancial support from the California Walnut Commission and Alexion; and nonfinancial support from the International Nut Council. J.S.-S. reports being a board member and personal fees from Instituto Danone Spain; being a board member and grants from the International Nut and Dried Fruit Foundation. R.E. reports being a board member of the Research Foundation on Wine and Nutrition, the Beer and Health Foundation, and the European Foundation for Alcohol Research; personal fees from KAO Corporation; lecture fees from Instituto Cervantes, Fundacion Dieta Mediterranea, Cerveceros de Espana, Lilly Laboratories, AstraZeneca, and Sanofi; and grants from Novartis, Amgen, Bicentury, and Grand Fountaine. A.P.-P. has served on advisory boards for Schwabe Farma Iberica. R.M.L.-R. reports personal fees from Cerveceros de Espana, UNIDECO, Adventia, Wine in Moderation, and Ecoveritas S.A., all outside the submitted work. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/16 CRDT- 2026/10/27 10:41 PHST- 2026/07/09 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ijms27209197 [pii] AID - ijms-27-09197 [pii] AID - 10.3390/ijms27209197 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 16;27(20):9197. doi: 10.3390/ijms27209197. PMID- 42941966 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 16 TI - Mapping and Evaluation of Quantitative Trait Loci for Adult-Plant Stripe Rust Resistance in the Sichuan Wheat Cultivar Chuanmai 62. LID - 10.3390/ijms27209196 [doi] LID - 9196 AB - Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), poses a persistent threat to wheat production in China. The Sichuan cultivar Chuanmai 62 (CM62) has exhibited stable adult-plant resistance (APR) for over a decade. To elucidate its genetic basis, we evaluated a recombinant inbred line population (CM62 x Avocet S) across 13 environments and genotyped it using the 55K SNP array. QTL mapping identified six QTL distributed on chromosomes 1BL, 2AS, 2BL, 5AL (two loci), and 5BL. Among these, three major QTL (QYr.CM62-1BL, QYr.CM62-2AS, and QYr.CM62-2BL) were detected. Notably, QYr.CM62-2BL was the preeminent locus, explaining 8.97-50.59% of phenotypic variance and exhibiting high stability across 11 of the 13 environments; this locus is closely linked to the previously reported QYR1 region, although whether it is allelic to QYR1 remains unclear. QYr.CM62-2AS corresponded to Yr17/YrM1225, while QYr.CM62-1BL aligned with Yr29. A significant finding was the identification of QYr.CM62-5BL, a novel minor-effect QTL mapped to the rearranged 5BL.7BL segment resulting from a 5B/7B reciprocal translocation present in both parents. Furthermore, two Kompetitive Allele-Specific PCR (KASP) markers (KASP-AX-109507945 and KASP-AX-109844182) were developed for QYr.CM62-2BL. Polymorphism screening of 328 cultivars confirmed their utility for marker-assisted selection. Collectively, our results demonstrate that the durable APR in CM62 is governed by multiple additive QTL. The newly developed KASP markers, together with the novel QYr.CM62-5BL locus and prioritized candidate genes from in silico annotation of the QYr.CM62-2BL interval, provide valuable genetic resources for pyramiding resistance alleles and future map-based cloning in wheat breeding programs. FAU - Yao, Fangjie AU - Yao F AD - Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. AD - Key Laboratory of Wheat Biology and Genetic Improvement on Southwestern China, Ministry of Agriculture and Rural Affairs of P.R.C., Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. AD - Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province, Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. FAU - Yang, Ennian AU - Yang E AD - Institute of Agricultural Resource and Environment, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. FAU - Li, Jun AU - Li J AD - Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. AD - Key Laboratory of Wheat Biology and Genetic Improvement on Southwestern China, Ministry of Agriculture and Rural Affairs of P.R.C., Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. AD - Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province, Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. FAU - Yang, Wuyun AU - Yang W AUID- ORCID: 0000-0002-8201-4929 AD - Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. AD - Key Laboratory of Wheat Biology and Genetic Improvement on Southwestern China, Ministry of Agriculture and Rural Affairs of P.R.C., Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. AD - Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province, Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. FAU - Yang, Manyu AU - Yang M AD - Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. AD - Key Laboratory of Wheat Biology and Genetic Improvement on Southwestern China, Ministry of Agriculture and Rural Affairs of P.R.C., Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. AD - Crop Germplasm Innovation and Genetic Improvement Key Laboratory of Sichuan Province, Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. LA - eng GR - SCCXTD-2024-11/Science and Technology Department of Sichuan Province/ GR - 2026YFHZ0069/Science and Technology Department of Sichuan Province/ GR - 1+9KJGG002/Sichuan Academy of Agricultural Sciences/ GR - 2026ZSSFGH01/Sichuan Academy of Agricultural Sciences/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - Puccinia striiformis SB - IM MH - *Quantitative Trait Loci MH - *Triticum/genetics/microbiology MH - *Disease Resistance/genetics MH - *Plant Diseases/microbiology/genetics MH - Chromosome Mapping MH - *Puccinia/pathogenicity MH - Basidiomycota/pathogenicity MH - Polymorphism, Single Nucleotide MH - Phenotype MH - Chromosomes, Plant/genetics MH - Genotype PMC - PMC13704936 OTO - NOTNLM OT - Chuanmai 62 OT - KASP marker OT - durable resistance OT - quantitative trait locus OT - stripe rust COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/16 CRDT- 2026/10/27 10:41 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ijms27209196 [pii] AID - ijms-27-09196 [pii] AID - 10.3390/ijms27209196 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 16;27(20):9196. doi: 10.3390/ijms27209196. PMID- 42941965 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 16 TI - Development and Genomic Validation of a Population-Specific SNP Panel for Parentage Verification in Chinese Holstein Cattle. LID - 10.3390/ijms27209198 [doi] LID - 9198 AB - Accurate pedigree information is essential for genetic evaluation and genomic selection in dairy cattle. However, the performance of SNP-based parentage verification depends on marker informativeness, genomic distribution, and compatibility with the target population. In this study, we developed and validated a population-specific 260-SNP panel for parentage verification in Chinese Holstein cattle. The panel was selected from BovineSNP150K genotype data of 2954 Chinese Holsteins based on high marker informativeness, genotyping quality, LD-based control of marker redundancy, and genome-wide distribution. The selected SNPs had a mean minor allele frequency of 0.4981, expected heterozygosity of 0.5000, and polymorphism information content of 0.3750, with a combined probability of exclusion >0.999. Parentage verification of 867 recorded candidate parent-offspring pairs using likelihood-based and Mendelian exclusion-based approaches showed 99.54% agreement, with a Cohen's kappa of 0.981. Random SNP-subset analyses showed greater variation in pedigree concordance estimates with smaller marker sets, whereas larger subsets produced more stable estimates across replicate subsets. Independent validation using targeted-capture sequencing showed a mean SNP detection rate of 99.96% and complete genotype concordance in duplicate tests. Independent validation across six commercial dairy farms identified an overall inconsistency rate of 12.97% between recorded pedigree relationships and genotype-based parentage evidence. These results demonstrate that the population-specific 260-SNP panel provides informative and reproducible genotyping for parentage verification in Chinese Holstein cattle and offers a practical tool for pedigree quality control in dairy cattle breeding programs. FAU - Dou, Jinhuan AU - Dou J AD - College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China. FAU - Gu, Jingyi AU - Gu J AD - College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China. FAU - Wang, Ao AU - Wang A AUID- ORCID: 0009-0005-8504-9739 AD - Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, National Engineering Laboratory of Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. FAU - Luo, Ziyu AU - Luo Z AD - College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China. FAU - Huang, Hetian AU - Huang H AD - College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China. FAU - Sheng, Xihui AU - Sheng X AD - College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China. FAU - Wang, Yachun AU - Wang Y AUID- ORCID: 0000-0003-3629-2802 AD - Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, National Engineering Laboratory of Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. FAU - Yu, Ying AU - Yu Y AD - Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, National Engineering Laboratory of Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. LA - eng GR - 32202644/National Natural Science Foundation of China/ GR - KM202310020007/Beijing Municipal Education Commission/ GR - 20250986/Beijing High Innovation Plan/ GR - QJKC2022027/Beijing University of Agriculture/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - Animals MH - *Polymorphism, Single Nucleotide MH - Cattle/genetics MH - Pedigree MH - *Genomics/methods MH - Genotype MH - Gene Frequency MH - Female MH - Breeding MH - Genome MH - Linkage Disequilibrium MH - Male PMC - PMC13705405 OTO - NOTNLM OT - Chinese Holstein cattle OT - genomic selection OT - parentage verification OT - pedigree accuracy OT - single nucleotide polymorphism OT - targeted sequencing COIS- J.D., Y.W., Y.Y., X.S., and H.H. are named inventors on Chinese Invention Patent No. ZL 2025 1 1515889.1 (Grant Publication No. CN 121182976 B), which is owned by Beijing University of Agriculture and is related to the SNP-based parentage-verification approach described in this study. The remaining authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/16 CRDT- 2026/10/27 10:41 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ijms27209198 [pii] AID - ijms-27-09198 [pii] AID - 10.3390/ijms27209198 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 16;27(20):9198. doi: 10.3390/ijms27209198. PMID- 42941964 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 16 TI - Autophagy in Pituitary Neuroendocrine Tumors (PitNETs): Molecular Mechanisms, Secretory Homeostasis, Therapeutic Resistance, and Translational Perspectives. LID - 10.3390/ijms27209195 [doi] LID - 9195 AB - Pituitary neuroendocrine tumors (PitNETs) are usually indolent but may show invasive growth, recurrence, hormonal hypersecretion, and treatment resistance. Autophagy intersects with nutrient sensing, lysosomal homeostasis, mitochondrial quality control, and secretory-granule turnover, but its biological effects in PitNETs are context-dependent. This narrative review synthesizes PitNET-specific and mechanistically relevant evidence on macroautophagy, mitophagy, crinophagy, non-coding RNA and epigenetic regulation, treatment resistance, and pharmacological modulation, emphasizing the distinction between static autophagy markers and dynamic autophagic flux. Evidence is interpreted across human tissue, primary human cells, pituitary tumor cell lines, animal models, and clinical observations, while preserving clear separation between human observations and mechanistic evidence derived from experimental models. Across experimental systems, autophagy activation is associated with antitumor effects in selected settings, whereas completed basal flux may support stress adaptation and late-stage or selective-autophagy inhibition may enhance cytotoxicity. Biological outcome therefore depends on tumor lineage, treatment, the pathway stage affected, and whether flux is completed. Recent studies extend this framework to selective mitophagy, ncRNA/exosomal resistance networks, epitranscriptomic regulation, somatostatin-analogue resistance, and tumor-microenvironmental signaling. Clinical translation remains limited, and no autophagy-modulating strategy is validated for routine PitNET management. Future studies should integrate dynamic flux measurements, lineage-specific biomarkers, pharmacodynamic target engagement, and prospective clinical endpoints. FAU - Rizzuto, Ida AU - Rizzuto I AUID- ORCID: 0009-0001-1234-3030 AD - Anatomic Pathology Unit, Department of Human Pathology in Adult and Developmental Age "Gaetano Barresi", University of Messina, 98125 Messina, Italy. FAU - Pepe, Ludovica AU - Pepe L AD - Anatomic Pathology Unit, Department of Human Pathology in Adult and Developmental Age "Gaetano Barresi", University of Messina, 98125 Messina, Italy. AD - PhD Program in Translational Molecular Medicine and Surgery, Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98125 Messina, Italy. FAU - Broggi, Giuseppe AU - Broggi G AUID- ORCID: 0000-0003-2576-6523 AD - Department of Medical and Surgical Sciences and Advanced Technologies "G.F. Ingrassia", Anatomic Pathology, University of Catania, 95123 Catania, Italy. FAU - Caltabiano, Rosario AU - Caltabiano R AUID- ORCID: 0000-0001-8591-8010 AD - Department of Medical and Surgical Sciences and Advanced Technologies "G.F. Ingrassia", Anatomic Pathology, University of Catania, 95123 Catania, Italy. FAU - Fiorentino, Vincenzo AU - Fiorentino V AUID- ORCID: 0000-0002-1132-1761 AD - Anatomic Pathology Unit, Department of Human Pathology in Adult and Developmental Age "Gaetano Barresi", University of Messina, 98125 Messina, Italy. AD - PhD Program in Translational Molecular Medicine and Surgery, Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98125 Messina, Italy. FAU - Ieni, Antonio AU - Ieni A AUID- ORCID: 0000-0003-2878-3572 AD - Anatomic Pathology Unit, Department of Human Pathology in Adult and Developmental Age "Gaetano Barresi", University of Messina, 98125 Messina, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - Humans MH - *Autophagy MH - *Neuroendocrine Tumors/metabolism/pathology/drug therapy/genetics MH - *Pituitary Neoplasms/metabolism/pathology/drug therapy/genetics MH - Animals MH - Homeostasis MH - *Drug Resistance, Neoplasm MH - Mitophagy MH - Translational Research, Biomedical PMC - PMC13705295 OTO - NOTNLM OT - PitNET OT - autophagic flux OT - autophagy OT - crinophagy OT - dopamine agonist OT - drug resistance OT - mitophagy OT - pituitary neuroendocrine tumor COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/16 CRDT- 2026/10/27 10:41 PHST- 2026/08/14 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ijms27209195 [pii] AID - ijms-27-09195 [pii] AID - 10.3390/ijms27209195 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 16;27(20):9195. doi: 10.3390/ijms27209195. PMID- 42941962 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 16 TI - ZEB1 Defines the Interaction Between Breast Cancer Cells and Macrophages by Upregulating the Expression of CCL2 Cytokine. LID - 10.3390/ijms27209194 [doi] LID - 9194 AB - The survival of disseminating tumor cells at the invasive edge and in metastatic foci depends on their ability to counteract anti-tumor immunity. The epithelial-mesenchymal transition (EMT) process is associated with the recruitment of immune-suppressive myeloid cells. The onset of the EMT gene expression program is controlled by several transcriptional master-regulators, including ZEB1. Using engineered breast cancer cell lines with different statuses of ZEB1, we examined the effects of ZEB1 on the expression of different pro-inflammatory cytokines. Our results suggest that ZEB1 plays an important role in regulating the expression of CCL2 (C-C motif ligand 2, a.k.a. MCP-1), the main chemoattractant of monocytes. Forced expression of ZEB1 in epithelial MCF-7 cells significantly increased the expression level of CCL2, which was further enhanced by TNF-alpha. On the contrary, knock-out of ZEB1 in pseudo-mesenchymal MDA-MB-231 cells led to a complete loss of CCL2 expression. Moreover, this effect was not restored by TNF-alpha induction. Also, positive ZEB1 status in breast cancer cells promoted the chemoattraction of THP-1 macrophages in co-culture experiments. Analysis of publicly available ChIP-seq data implicated ZEB1 in the regulation of CCL2 gene transcription. Taken together, these data suggest that ZEB1 is involved in the formation of the EMT-associated pro-tumor immune microenvironment. FAU - Semenov, Oleg AU - Semenov O AUID- ORCID: 0000-0003-2042-361X AD - Institute of Cytology of the Russian Academy of Sciences, St. Petersburg 194064, Russia. FAU - Nevzorov, Ivan AU - Nevzorov I AUID- ORCID: 0000-0002-1224-5772 AD - Institute of Cytology of the Russian Academy of Sciences, St. Petersburg 194064, Russia. FAU - Silonov, Sergei AU - Silonov S AUID- ORCID: 0000-0001-8714-6659 AD - Institute of Cytology of the Russian Academy of Sciences, St. Petersburg 194064, Russia. FAU - Daks, Alexandra AU - Daks A AUID- ORCID: 0000-0003-0495-1244 AD - Institute of Cytology of the Russian Academy of Sciences, St. Petersburg 194064, Russia. FAU - Kabasheva, Aliya AU - Kabasheva A AUID- ORCID: 0009-0004-3763-9622 AD - Department of Biomedical sciences, School of Medicine, Nazarbayev University, Astana 010000, Kazakhstan. AD - National Laboratory of Astana, Astana 010000, Kazakhstan. FAU - Nazarov, Alexander AU - Nazarov A AUID- ORCID: 0000-0003-4368-4011 AD - Institute of Cytology of the Russian Academy of Sciences, St. Petersburg 194064, Russia. FAU - Kriger, Daria AU - Kriger D AUID- ORCID: 0000-0002-7147-1884 AD - Institute of Cytology of the Russian Academy of Sciences, St. Petersburg 194064, Russia. FAU - Kuneev, Ivan AU - Kuneev I AUID- ORCID: 0000-0002-5918-572X AD - Institute of Cytology of the Russian Academy of Sciences, St. Petersburg 194064, Russia. FAU - Petukhov, Alexey AU - Petukhov A AD - Department of Biomedical sciences, School of Medicine, Nazarbayev University, Astana 010000, Kazakhstan. FAU - Gnennaya, Yulia AU - Gnennaya Y AUID- ORCID: 0000-0002-3571-333X AD - Institute of Cytology of the Russian Academy of Sciences, St. Petersburg 194064, Russia. FAU - Tulchinsky, Eugene AU - Tulchinsky E AD - Department of Biomedical sciences, School of Medicine, Nazarbayev University, Astana 010000, Kazakhstan. FAU - Shuvalov, Oleg AU - Shuvalov O AUID- ORCID: 0000-0002-8352-5683 AD - Institute of Cytology of the Russian Academy of Sciences, St. Petersburg 194064, Russia. FAU - Barlev, Nickolai AU - Barlev N AUID- ORCID: 0000-0001-7111-2446 AD - Department of Biomedical sciences, School of Medicine, Nazarbayev University, Astana 010000, Kazakhstan. AD - National Laboratory of Astana, Astana 010000, Kazakhstan. LA - eng GR - grant# AP23486728 to N.A.B/Committee of Science of the Ministry of Science and Higher Education of the Republic of Kazakhstan/ GR - 211123CRP1601 to N.A.B/Nazarbayev University/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Zinc Finger E-box-Binding Homeobox 1) RN - 0 (Chemokine CCL2) RN - 0 (ZEB1 protein, human) RN - 0 (CCL2 protein, human) RN - 0 (Tumor Necrosis Factor-alpha) SB - IM MH - Humans MH - *Zinc Finger E-box-Binding Homeobox 1/metabolism/genetics MH - *Chemokine CCL2/genetics/metabolism MH - *Breast Neoplasms/metabolism/genetics/pathology MH - Female MH - *Macrophages/metabolism MH - Gene Expression Regulation, Neoplastic MH - Epithelial-Mesenchymal Transition/genetics MH - MDA-MB-231 Cells MH - Cell Line, Tumor MH - MCF-7 Cells MH - Up-Regulation MH - Tumor Necrosis Factor-alpha/metabolism MH - THP-1 Cells MH - Coculture Techniques PMC - PMC13705571 OTO - NOTNLM OT - CCL2 OT - EMT OT - ZEB1 OT - breast cancer OT - macrophages OT - tumor microenvironment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/16 CRDT- 2026/10/27 10:41 PHST- 2026/05/05 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ijms27209194 [pii] AID - ijms-27-09194 [pii] AID - 10.3390/ijms27209194 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 16;27(20):9194. doi: 10.3390/ijms27209194. PMID- 42941959 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 16 TI - EP2 and EP4 in Alzheimer's Disease: Cell-Dependent Signalling and Implications for Selective Targeting. LID - 10.3390/ijms27209190 [doi] LID - 9190 AB - Prostaglandin E2 (PGE(2)) signalling influences amyloid-beta (Abeta) processing, myeloid function, and neuronal injury in Alzheimer's disease (AD). Although EP2 and EP4 share Gs-cyclic adenosine monophosphate (cAMP) signalling, their cellular effects differ. This review integrates genetic, pharmacological, ageing, and AD-model evidence to assess receptor-selective targeting. EP2 deletion enhances myeloid Abeta handling and reduces microglia-mediated neuronal injury in experimental systems, providing a rationale for selective EP2 inhibition. In aged mice, PGE(2)-EP2 signalling redirects glucose towards glycogen storage and restricts myeloid mitochondrial respiration; benefits from a predominantly peripheral antagonist suggest that some age-related outcomes may improve without high brain exposure. Chronic antagonist studies in AD models, however, show variable effects on inflammation, amyloid burden, and behaviour. EP4 exhibits contrasting cell-dependent actions: activation supports microglial Abeta uptake and inflammatory control, whereas signalling in some amyloid precursor protein (APP)-expressing cells promotes APP internalisation and Abeta production. Human AD evidence remains mainly tissue-based; EP2 antagonist testing has involved healthy volunteers, not patients with AD. These findings indicate that receptor selectivity alone is insufficient to predict therapeutic outcomes. Further studies should combine cell-specific receptor manipulation, measurements of central and peripheral exposure, and separate assessments of Abeta production, clearance, tissue injury, and functional outcomes. FAU - Zhao, Xi AU - Zhao X AUID- ORCID: 0009-0008-8332-9835 AD - School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, No. 300 Xueshi Road, Yuelu District, Changsha 410208, China. AD - Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Zeng, Youjia AU - Zeng Y AD - School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, No. 300 Xueshi Road, Yuelu District, Changsha 410208, China. AD - Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Song, Zhenyan AU - Song Z AUID- ORCID: 0000-0003-0758-7484 AD - School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, No. 300 Xueshi Road, Yuelu District, Changsha 410208, China. AD - Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - He, Jiawei AU - He J AD - School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, No. 300 Xueshi Road, Yuelu District, Changsha 410208, China. AD - Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - He, Qijun AU - He Q AD - School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, No. 300 Xueshi Road, Yuelu District, Changsha 410208, China. AD - Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Lin, Han AU - Lin H AD - School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, No. 300 Xueshi Road, Yuelu District, Changsha 410208, China. AD - Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Liu, Lu AU - Liu L AUID- ORCID: 0009-0003-3564-8085 AD - School of Integrated Chinese and Western Medicine, Hunan University of Chinese Medicine, No. 300 Xueshi Road, Yuelu District, Changsha 410208, China. AD - Key Laboratory of Hunan Province for Integrated Traditional Chinese and Western Medicine on Prevention and Treatment of Cardio-Cerebral Diseases, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Yu, Wenjing AU - Yu W AD - Medical School, Hunan University of Chinese Medicine, No. 300 Xueshi Road, Yuelu District, Changsha 410208, China. FAU - Cheng, Shaowu AU - Cheng S AUID- ORCID: 0000-0001-6148-9565 AD - Medical School, Hunan University of Chinese Medicine, No. 300 Xueshi Road, Yuelu District, Changsha 410208, China. LA - eng GR - 82074046/National Natural Science Foundation of China/ GR - 82575038/National Natural Science Foundation of China/ GR - 2024RC1060/the Science and Technology Innovation Leading Talent Program of the Hunan Provincial Department of Science and Technology/ GR - 2025JJ90003/the University Joint Fund of the Natural Science Foundation of Hunan Province/ GR - Internal Medicine of Traditional Chinese Medicine; Document No. Xiang Wei Yi Fa [2025] 7/the 2025 Hunan Provincial Class A Medical Discipline Construction Project/ GR - CX20251181/the Postgraduate Scientific Research Innovation Project of Hunan Province/ GR - 2025CX071/Postgraduate Scientific Research Innovation Project of Hunan Province/ PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Receptors, Prostaglandin E, EP2 Subtype) RN - 0 (Receptors, Prostaglandin E, EP4 Subtype) RN - 0 (Amyloid beta-Peptides) RN - K7Q1JQR04M (Dinoprostone) SB - IM MH - *Alzheimer Disease/metabolism/drug therapy/pathology MH - Humans MH - *Receptors, Prostaglandin E, EP2 Subtype/metabolism/antagonists & inhibitors/genetics MH - Animals MH - *Receptors, Prostaglandin E, EP4 Subtype/metabolism/antagonists & inhibitors/genetics MH - *Signal Transduction MH - Amyloid beta-Peptides/metabolism MH - Dinoprostone/metabolism MH - Microglia/metabolism PMC - PMC13705105 OTO - NOTNLM OT - Alzheimer's disease OT - EP2 OT - EP4 OT - ageing OT - microglia OT - myeloid metabolism OT - prostaglandin E2 COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/16 CRDT- 2026/10/27 10:41 PHST- 2026/10/04 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ijms27209190 [pii] AID - ijms-27-09190 [pii] AID - 10.3390/ijms27209190 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 16;27(20):9190. doi: 10.3390/ijms27209190. PMID- 42941957 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 16 TI - Sleep Disturbances and Circadian Phase Delay in People Living with Human Immunodeficiency Virus (HIV): A Systematic Review. LID - 10.3390/ijms27209189 [doi] LID - 9189 AB - Advances in HIV research and antiretroviral therapy (ART) have substantially increased life expectancy among people living with HIV (PLHIV). However, challenges affecting quality of life and alterations in daily physiological processes persist. Among the health dimensions most affected are sleep quality and circadian rhythms, resulting in significant biological alterations. The main objective of this study is to analyze the existing scientific evidence regarding the effects of HIV on sleep quality and on the alignment and synchronization of circadian rhythms. The bidirectional interaction between these physiological alterations and associated clinical, immunological, and metabolic factors in people living with HIV (PLHIV) was evaluated, emphasizing their integration. For this review, searches were conducted in the PubMed, Scopus, and Web of Science databases. Eligibility criteria were applied, resulting in a final selection of 15 studies based on search strategies. The findings demonstrated a high prevalence of poor sleep quality as well as delayed circadian phase. These chronobiological alterations were closely associated with systemic inflammation, altered melatonin levels, impaired cognitive performance, and an altered metabolic profile involving leptin, ghrelin, adiponectin, and peroxisome proliferator-activated receptor (PPAR) signaling. Despite the consistency of the results, the methodologies of individual studies varied, and a notable absence of clinical trials aimed at addressing poor sleep quality and chronodisruption was observed. A promising clinical approach involves complementing antiretroviral therapy (ART) with chronotherapeutic interventions and sleep hygiene. In conclusion, HIV can disrupt personal temporal organization at a systemic level; consequently, incorporating assessments of sleep and circadian rhythms into clinical care may be warranted, while chronotherapeutic interventions should be further investigated. FAU - Gomila-Cardona, Antoni AU - Gomila-Cardona A AD - Department of Biology, University of the Balearic Islands (UIB), 07122 Palma, Spain. FAU - Ferrandez-Sempere, Saul AU - Ferrandez-Sempere S AUID- ORCID: 0009-0008-2987-6926 AD - Department of Biology, University of the Balearic Islands (UIB), 07122 Palma, Spain. FAU - Canellas, Francesca AU - Canellas F AD - Department of Biology, University of the Balearic Islands (UIB), 07122 Palma, Spain. AD - Health Research Institute of the Balearic Islands (IdISBa), 07010 Palma, Spain. AD - Son Espases University Hospital (HUSE), 07010 Palma, Spain. FAU - Gamundi, Antoni AU - Gamundi A AUID- ORCID: 0000-0002-0880-4658 AD - Department of Biology, University of the Balearic Islands (UIB), 07122 Palma, Spain. AD - Health Research Institute of the Balearic Islands (IdISBa), 07010 Palma, Spain. FAU - Akaarir, Mourad AU - Akaarir M AD - Department of Biology, University of the Balearic Islands (UIB), 07122 Palma, Spain. AD - Health Research Institute of the Balearic Islands (IdISBa), 07010 Palma, Spain. FAU - Nicolau, Cristina AU - Nicolau C AUID- ORCID: 0000-0001-5174-4323 AD - Department of Biology, University of the Balearic Islands (UIB), 07122 Palma, Spain. AD - Health Research Institute of the Balearic Islands (IdISBa), 07010 Palma, Spain. FAU - Rubino-Diaz, Jose Angel AU - Rubino-Diaz JA AUID- ORCID: 0000-0001-6898-4665 AD - Department of Biology, University of the Balearic Islands (UIB), 07122 Palma, Spain. AD - Health Research Institute of the Balearic Islands (IdISBa), 07010 Palma, Spain. AD - Son Espases University Hospital (HUSE), 07010 Palma, Spain. AD - Nursing School, CESAG-UP Comillas (University College Alberta Gimenez affiliated to Comillas Pontifical University), 07013 Palma, Spain. LA - eng PT - Journal Article PT - Review PT - Systematic Review DEP - 20261016 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - JL5DK93RCL (Melatonin) SB - IM MH - Humans MH - *HIV Infections/complications/physiopathology/drug therapy MH - *Circadian Rhythm/physiology MH - *Sleep Wake Disorders/etiology MH - Sleep Quality MH - HIV MH - Melatonin/metabolism MH - Quality of Life PMC - PMC13705161 OTO - NOTNLM OT - HIV OT - circadian rhythms OT - human immunodeficiency virus OT - people living with HIV OT - sleep quality OT - sleep wake disorders COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/16 CRDT- 2026/10/27 10:41 PHST- 2026/09/21 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ijms27209189 [pii] AID - ijms-27-09189 [pii] AID - 10.3390/ijms27209189 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 16;27(20):9189. doi: 10.3390/ijms27209189. PMID- 42941954 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 16 TI - Disentangling the Effects of Craniotomy and Traumatic Brain Injury in Experimental Models: A Narrative Review. LID - 10.3390/ijms27209186 [doi] LID - 9186 AB - Many widely used open-head models of traumatic brain injury (TBI) require craniotomy before injury, while sham-operated animals undergo surgery without impact and serve as controls. However, sham surgery itself may alter physiological, molecular, and behavioral parameters, complicating interpretation of TBI-specific effects. This narrative review, based on a structured PubMed search, synthesizes studies employing a three-group design to distinguish injury-related changes from those associated with craniotomy and other perioperative procedures. Evidence indicates that sham surgery produces outcome-, region-, and time-dependent effects that are generally milder, more localized, and more transient than TBI. These include neuroinflammatory responses, localized neuronal alterations, temporary sensorimotor and cognitive deficits, nociceptive changes, and shifts in gut microbiota. In contrast, TBI causes more severe abnormalities, including extensive neuronal damage, sustained astrogliosis, microglial activation, neutrophilic infiltration, mitochondrial dysfunction, and long-term dysbiosis. Most electrophysiological parameters are relatively unaffected by sham surgery, although selected changes have been reported. Importantly, differences between naive and sham animals cannot be attributed exclusively to craniotomy, as anesthesia, analgesia, and surgical technique may contribute. Thus, sham surgery should not be considered a biologically neutral control. Inclusion of both naive and sham groups is particularly important when distinguishing surgery-associated from injury-specific effects in preclinical studies. FAU - Gribovskaya, Viktoria AU - Gribovskaya V AUID- ORCID: 0009-0006-5406-4255 AD - Department of Genetics, Cytology, and Bioengineering, Voronezh State University, Voronezh 394018, Russia. FAU - Adzhemian, Dianna AU - Adzhemian D AUID- ORCID: 0009-0004-7968-3410 AD - Department of Genetics, Cytology, and Bioengineering, Voronezh State University, Voronezh 394018, Russia. FAU - Rogozin, Pavel AU - Rogozin P AUID- ORCID: 0000-0002-5735-6898 AD - Brain Science Institute, Russian Center of Neurology and Neurosciences, Moscow 125367, Russia. FAU - Nesterova, Veronika AU - Nesterova V AUID- ORCID: 0009-0000-1069-9108 AD - Department of Genetics, Cytology, and Bioengineering, Voronezh State University, Voronezh 394018, Russia. FAU - Gureev, Artem AU - Gureev A AUID- ORCID: 0000-0003-3562-5329 AD - Department of Genetics, Cytology, and Bioengineering, Voronezh State University, Voronezh 394018, Russia. FAU - Silachev, Denis AU - Silachev D AUID- ORCID: 0000-0003-0581-9755 AD - Kulakov National Medical Research Center of Obstetrics, Gynecology and Perinatology, 4 Akademika Oparina Str., Moscow 117997, Russia. AD - Department of Functional Biochemistry of Biopolymers, A.N. Belozersky Research Institute of Physico-Chemical Biology, Moscow State University, Moscow 119992, Russia. LA - eng GR - 26-75-10105/Russian Science Foundation/ PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - *Brain Injuries, Traumatic/pathology/physiopathology/surgery MH - Animals MH - *Craniotomy/adverse effects/methods MH - Humans MH - Disease Models, Animal PMC - PMC13705088 OTO - NOTNLM OT - biochemical markers OT - cognitive impairment OT - craniotomy OT - inflammation OT - naive OT - sham OT - traumatic brain injury COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/16 CRDT- 2026/10/27 10:41 PHST- 2026/09/13 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ijms27209186 [pii] AID - ijms-27-09186 [pii] AID - 10.3390/ijms27209186 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 16;27(20):9186. doi: 10.3390/ijms27209186. PMID- 42941947 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 16 TI - Integrative Genetic and Transcriptomic Analysis Reveals Candidate Molecular Differences Among Dementia with Lewy Bodies, Alzheimer's Disease, and Parkinson's Disease Dementia. LID - 10.3390/ijms27209173 [doi] LID - 9173 AB - Dementia with Lewy bodies (DLB) shares substantial clinical and molecular features with Alzheimer's disease (AD) and Parkinson's disease dementia (PDD), complicating the identification of disease-specific molecular alterations. This study aimed to characterize transcriptomic differences among these disorders and to integrate expression findings with published Lewy body dementia (LBD) genetic evidence. Gene-level expression profiles from 42 postmortem prefrontal cortex samples in GSE150696, including controls, AD, DLB, and PDD, were analyzed using age- and sex-adjusted empirical-Bayes models with false discovery rate (FDR) correction, followed by sensitivity analyses, post hoc exploratory candidate selection, and integration with an external LBD multi-omics gene-prioritization dataset. No gene reached FDR < 0.05 in the DLB-versus-AD comparison. In contrast, SLC19A2, VAMP1, and COX5B were lower in DLB than in PDD and were FDR-supported in the primary age- and sex-adjusted model, although their statistical significance was partly model-dependent. A post hoc cross-disease analysis identified 60 exploratory candidates, of which SYT11 was the only gene overlapping with the published LBD-prioritized gene set. The overall 60-gene set was not significantly enriched for LBD-associated genes. These findings did not demonstrate broad robust disease-disease differences and are consistent with substantial molecular overlap, while remaining compatible with limited statistical power. A limited number of transcriptomic candidates, including the exploratory SYT11 overlap, warrant independent validation. FAU - Oztan, Gozde AU - Oztan G AUID- ORCID: 0000-0002-2970-1834 AD - Department of Medical Biology, Istanbul Faculty of Medicine, Istanbul University, Topkapi, 34093 Istanbul, Turkey. FAU - Issever, Tugce AU - Issever T AUID- ORCID: 0000-0002-9059-8709 AD - Turkish Health Institutes Presidency (TUSEB), 34718 Istanbul, Turkey. FAU - Issever, Halim AU - Issever H AUID- ORCID: 0000-0002-5435-706X AD - Department of Public Health, Istanbul Faculty of Medicine, Istanbul University, Topkapi, 34093 Istanbul, Turkey. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - Humans MH - *Lewy Body Disease/genetics/metabolism MH - *Alzheimer Disease/genetics/metabolism MH - *Parkinson Disease/genetics/metabolism MH - *Gene Expression Profiling MH - *Transcriptome MH - Female MH - Male MH - Aged PMC - PMC13705459 OTO - NOTNLM OT - Alzheimer's disease OT - COX5B OT - Parkinson's disease dementia OT - SLC19A2 OT - SYT11 OT - VAMP1 OT - dementia with Lewy bodies OT - differential gene expression OT - genetic integration OT - transcriptomics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/16 CRDT- 2026/10/27 10:41 PHST- 2026/09/19 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ijms27209173 [pii] AID - ijms-27-09173 [pii] AID - 10.3390/ijms27209173 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 16;27(20):9173. doi: 10.3390/ijms27209173. PMID- 42941946 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 16 TI - Analysis of Melatonin Secretion Patterns in Buffalo Milk and Its Association with AANAT Polymorphisms. LID - 10.3390/ijms27209178 [doi] LID - 9178 AB - Melatonin (MT) is an important bioactive functional component of milk, where its content and biosynthetic mechanism are of considerable relevance to the quality regulation of dairy buffalo milk. This study aimed to investigate the variation patterns and biosynthetic mechanisms of melatonin in milk from dairy buffaloes. A total of 125 buffalo milk samples were collected, and MT content was determined using liquid chromatography-tandem mass spectrometry (LC-MS/MS). MT secretion patterns were evaluated according to breed, lactation, parity, and milking time (light exposure). In addition, whole-genome sequencing (WGS) data were used to screen for single-nucleotide polymorphisms (SNPs), which are significantly associated with melatonin biosynthetic enzyme genes. The results showed that breed, milking time, and lactation were the major factors affecting MT content in buffalo milk. Based on the WGS data, two SNPs significantly associated with MT secretion and synthesis were identified within the aralkylamine N-acetyltransferase (AANAT) region, among which the GG genotype at 3-8019407 and the CC genotype at 3-8019446 were associated with higher MT content. The MT content for both wild-type homozygotes was 59.54 pg/mL, which was higher than that of heterozygotes (39.12 pg/mL) (p < 0.05). With the exception of SNP2 and SNP8, all other SNPs were in Hardy-Weinberg equilibrium. These findings will serve as a reference for the subsequent selection and identification of breeding materials for high MT dairy buffalo, as well as for the development of high MT buffalo milk products. FAU - You, Songyuan AU - You S AD - Guangxi Zhuang Autonomous Region Buffalo Milk Quality and Safety Control Technology Engineering Research Center, Guangxi Buffalo Research Institute, Nanning 530001, China. FAU - Fu, Yiheng AU - Fu Y AD - Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, China. FAU - Li, Yanghua AU - Li Y AD - Guangxi Zhuang Autonomus Region Center for Analysis and Test Research, Nanning 530022, China. FAU - Jiang, Jiaqi AU - Jiang J AD - Guangxi Zhuang Autonomous Region Buffalo Milk Quality and Safety Control Technology Engineering Research Center, Guangxi Buffalo Research Institute, Nanning 530001, China. FAU - Hau, Eng Huan AU - Hau EH AD - Guangxi Zhuang Autonomous Region Buffalo Milk Quality and Safety Control Technology Engineering Research Center, Guangxi Buffalo Research Institute, Nanning 530001, China. FAU - Wei, Xingqi AU - Wei X AD - Guangxi Key Laboratory of Animal Reproduction, Breeding and Disease Control, College of Animal Science and Technology, Guangxi University, Nanning 530004, China. FAU - Long, Zhixiang AU - Long Z AD - Guangxi Zhuang Autonomus Region Center for Analysis and Test Research, Nanning 530022, China. FAU - Zeng, Qingkun AU - Zeng Q AUID- ORCID: 0000-0003-1575-9565 AD - Guangxi Zhuang Autonomous Region Buffalo Milk Quality and Safety Control Technology Engineering Research Center, Guangxi Buffalo Research Institute, Nanning 530001, China. FAU - Liu, Qingyou AU - Liu Q AD - College of Animal Science, South China Agricultural University, Guangzhou 510640, China. FAU - Li, Ling AU - Li L AUID- ORCID: 0000-0003-2948-6561 AD - Guangxi Zhuang Autonomous Region Buffalo Milk Quality and Safety Control Technology Engineering Research Center, Guangxi Buffalo Research Institute, Nanning 530001, China. LA - eng GR - 32460814/Regional Science Fund Project of the National Natural Science Foundation of China/ GR - 32530099/Key Program of the National Natural Science Foundation of China/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - JL5DK93RCL (Melatonin) RN - EC 2.3.1.87 (Arylalkylamine N-Acetyltransferase) SB - IM MH - Animals MH - *Melatonin/metabolism/genetics MH - *Buffaloes/genetics/metabolism MH - *Milk/metabolism/chemistry MH - *Polymorphism, Single Nucleotide MH - Female MH - *Arylalkylamine N-Acetyltransferase/genetics/metabolism MH - Genotype MH - Lactation/genetics MH - Tandem Mass Spectrometry PMC - PMC13704996 OTO - NOTNLM OT - AANAT OT - SNP OT - buffalo milk OT - melatonin OT - secretion pattern COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/16 CRDT- 2026/10/27 10:41 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ijms27209178 [pii] AID - ijms-27-09178 [pii] AID - 10.3390/ijms27209178 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 16;27(20):9178. doi: 10.3390/ijms27209178. PMID- 42941941 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 16 TI - RNA Interference-Mediated Suppression of RAGE. LID - 10.3390/ijms27209167 [doi] LID - 9167 AB - The receptor for advanced glycation end products (RAGE/AGER) is an important mediator of inflammatory signaling and has been implicated in chronic low-grade inflammation associated with aging. Chronic low-grade inflammation ("inflammaging") is a characteristic feature of biological aging, providing a rationale for investigating molecular regulators of inflammatory signaling such as RAGE. This study aimed to develop and validate short hairpin RNA (shRNA) constructs suppressing AGER/RAGE in human cell models. Five shRNA constructs targeting human AGER were designed, cloned into pLSL-puro, and verified by Sanger sequencing. Lentiviral vectors were produced in HEK293T cells and used to establish stable HEK293T, 786-O, and human umbilical vein endothelial cell (HUVEC) populations with AGER knockdown. AGER/RAGE expression was assessed by RT-qPCR, Western blotting, and flow cytometry. Silencing efficiency varied with the construct and the cellular model. sh1 produced the strongest suppression of AGER, exceeding 70% in selected models, whereas sh2 was effective in HEK293T and 786-O cells. Reduced RAGE expression was confirmed at the mRNA, protein, and cell-surface levels, identifying sh1 and sh2 as lead candidates. An AGER-focused workflow for lentiviral shRNA construct screening and experimental validation was established, providing a reproducible approach for generating stable cell models with reduced AGER/RAGE expression. Further work is required to confirm isoform specificity, assess off-target effects, and define the functional consequences of RAGE suppression. FAU - Muzaffarova, Tatiana A AU - Muzaffarova TA AD - Institute of Biology of Aging and Medicine of Healthy Longevity with the Clinic of Predictive Medicine, Petrovsky National Research Centre of Surgery, 119435 Moscow, Russia. FAU - Malinovskaya, Elena M AU - Malinovskaya EM AD - Institute of Biology of Aging and Medicine of Healthy Longevity with the Clinic of Predictive Medicine, Petrovsky National Research Centre of Surgery, 119435 Moscow, Russia. FAU - Moskalev, Alexey AU - Moskalev A AD - Institute of Biology of Aging and Medicine of Healthy Longevity with the Clinic of Predictive Medicine, Petrovsky National Research Centre of Surgery, 119435 Moscow, Russia. LA - eng GR - 125090910147-7/Russian Ministry of Science and Higher Education/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Receptor for Advanced Glycation End Products) RN - 0 (RNA, Small Interfering) RN - 0 (AGER protein, human) SB - IM MH - Humans MH - *Receptor for Advanced Glycation End Products/genetics/metabolism/antagonists & inhibitors MH - HEK293 Cells MH - *RNA Interference MH - RNA, Small Interfering/genetics MH - Human Umbilical Vein Endothelial Cells MH - Lentivirus/genetics PMC - PMC13705484 OTO - NOTNLM OT - AGER OT - RNA interference OT - aging biology OT - endothelial cells OT - gene silencing OT - inflammation OT - lentiviral vectors OT - receptor for advanced glycation end products OT - short hairpin RNA COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/16 CRDT- 2026/10/27 10:41 PHST- 2026/09/18 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ijms27209167 [pii] AID - ijms-27-09167 [pii] AID - 10.3390/ijms27209167 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 16;27(20):9167. doi: 10.3390/ijms27209167. PMID- 42941930 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 15 TI - Distinct Post-Exposure Metabolic States Following Pharmacological Perturbation of Mitochondrial Respiratory Complexes I-V in SH-SY5Y Cells: A Positional (13)C-NMR Study. LID - 10.3390/ijms27209160 [doi] LID - 9160 AB - Mitochondrial dysfunction is a recurring mechanism in neuronal vulnerability and in the toxicity of several neuroactive compounds, yet lesions at different respiratory-chain nodes are commonly treated as a single bioenergetic defect. Whether pharmacological perturbation at distinct nodes leaves neuronal cells in distinguishable metabolic states has not been examined across all five complexes in one system. Undifferentiated SH-SY5Y human neuroblastoma cells were exposed to five compounds selected as pharmacological perturbagens nominally directed at Complexes I-V: MPTP, 3-nitropropionic acid (3-NP), antimycin A, sodium azide and oligomycin, each titrated to approximately 80% viability (24 h, except 3-NP at 12 h). Inhibitors were then removed, and cells were labelled for 24 h with [U-(13)C(6)]glucose. Positional (13)C-NMR was combined with pyruvate carboxylase (PC) and pyruvate dehydrogenase (PDH) enzyme-capacity assays in parallel extracts from the same biological replicates (n = 3 per group). The design is a single-factor, six-level layout in which five exposures share one vehicle control; labelling data were log2-transformed and analysed by one-way ANOVA with Dunnett many-to-one contrasts (error df = 12), with Hedges' g and 95% confidence intervals, and group structure was tested by permutational multivariate analysis of variance (PERMANOVA). PERMANOVA demonstrated a significant overall effect of exposure group on the multivariate labelling matrix (pseudo-F = 18.77, p = 0.0001, 9999 permutations), and principal component analysis of the seven complete-case metabolites showed visually distinct group structure (PC1 65.9%, PC2 19.3%; 85.3% cumulative). Oligomycin produced the most distinct positional phenotype, preserving the PDH-associated glutamate C4 readout (107.2% of control, p = 0.971) while nearly doubling the PC-associated glutamate C3 readout (197.7%, p = 0.004; Hedges' g = 17.8, 95% CI 5.3-30.2), concordant with PC capacity at approximately 138% and PDH capacity at approximately 60% of control. Azide produced the strongest reduction in glutamate C4 labelling (31.9%, p < 0.001; g = -8.3); antimycin A produced the broadest suppression of labelling; 3-NP produced accumulation of (13)C-labelled succinate that was undetectable in the control. MPTP gave the weakest phenotype, with most of its individual comparisons not surviving the shared-control analysis. Exposure to compounds directed at different respiratory nodes leaves neuronal cells in non-equivalent post-exposure (13)C-labelling states that positional (13)C-NMR resolves. Because tracing followed washout, because the compounds are not fully selective and because the enzyme assays report capacity rather than flux, these are compound-defined post-exposure metabolic states and not measurements of complex-specific flux. FAU - Li, Hong AU - Li H AD - Center for Advanced Metabolomics and Proteomics Research, Department of Microbiology, Biochemistry, and Molecular Genetics, New Jersey Medical School, Rutgers University, Newark, NJ 07103, USA. FAU - Baykal, Ahmet Tarik AU - Baykal AT AUID- ORCID: 0000-0002-8814-7351 AD - Department of Medical Biochemistry, Faculty of Medicine, Acibadem Mehmet Ali Aydinlar University, Istanbul 34450, Turkey. AD - Department of Biochemistry and Molecular Biology, Graduate School of Health Sciences, Acibadem Mehmet Ali Aydinlar University, Istanbul 34450, Turkey. AD - Acibadem Labmed Clinical Laboratories, Istanbul 34450, Turkey. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - QY4L0FOX0D (3-nitropropionic acid) RN - 0 (Nitro Compounds) RN - 642-15-9 (Antimycin A) RN - 0 (Oligomycins) RN - EC 7.1.1.2 (Electron Transport Complex I) RN - 0 (Propionates) RN - 968JJ8C9DV (Sodium Azide) RN - 0 (Pyruvate Dehydrogenase Complex) RN - EC 6.4.1.1 (Pyruvate Carboxylase) SB - IM MH - Humans MH - *Mitochondria/metabolism/drug effects MH - Cell Line, Tumor MH - Nitro Compounds/pharmacology MH - Antimycin A/pharmacology MH - Oligomycins/pharmacology MH - *Electron Transport Complex I/metabolism MH - Propionates/pharmacology MH - Sodium Azide/pharmacology MH - Pyruvate Dehydrogenase Complex/metabolism MH - Electron Transport/drug effects MH - Pyruvate Carboxylase/metabolism MH - Carbon-13 Magnetic Resonance Spectroscopy PMC - PMC13705396 OTO - NOTNLM OT - 13C NMR metabolomics OT - SH-SY5Y neuroblastoma OT - anaplerosis OT - electron transport chain OT - inhibitor polypharmacology OT - mitochondrial neurotoxicity OT - positional 13C-NMR OT - pyruvate carboxylase OT - respiratory-chain pharmacology OT - stable isotope-resolved metabolomics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/15 CRDT- 2026/10/27 10:41 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ijms27209160 [pii] AID - ijms-27-09160 [pii] AID - 10.3390/ijms27209160 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 15;27(20):9160. doi: 10.3390/ijms27209160. PMID- 42941929 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 15 TI - Multiscale Computational Prioritization of Chromone-Based Acetylcholinesterase Ligands. LID - 10.3390/ijms27209153 [doi] LID - 9153 AB - Identifying acetylcholinesterase (AChE) ligands for Alzheimer's disease research requires integrating molecular recognition with pharmacokinetic and safety considerations. Here, four designed chromone derivatives (M1-M4) were investigated to determine how conformational sampling and complementary molecular descriptors refine docking-based prioritization. Donepezil redocking in human AChE and cross-docking into Torpedo californica AChE recovered the crystallographic and transferred reference orientations, respectively, with RMSDs of 0.942 and 1.074 A. Molecular docking was integrated with 800 ns molecular dynamics, MM/PBSA, electronic-structure calculations, and pharmacokinetic/toxicity predictions. M4 achieved the most favorable chromone docking score (-9.6 kcal mol(-1)), whereas M3 yielded the most favorable chromone MM/PBSA estimate within the 775-800 ns window (-23.90 +/- 2.31 kcal mol(-1)). Donepezil retained a more favorable MM/PBSA estimate (-41.00 +/- 2.75 kcal mol(-1)). M4 exhibited the highest electronic hardness, while predicted toxicity profiles revealed distinct liabilities across the series. The divergence between docking and sampled energetics highlights the value of integrating complementary criteria when selecting compounds for experimental evaluation. These protocol-dependent findings prioritize M3 and M4 as complementary computational candidates requiring experimental confirmation of AChE inhibition, selectivity, and safety. FAU - Khibech, Oussama AU - Khibech O AUID- ORCID: 0009-0007-9407-7576 AD - Laboratory of Materials Chemistry and Applications (LCMA), Department of Chemistry, Faculty of Sciences, Mohammed Premier University, BP 717, Oujda 60000, Morocco. FAU - Abdelfattah, Bahia AU - Abdelfattah B AUID- ORCID: 0009-0008-8311-2513 AD - Laboratory of Physical Chemistry of Materials, Natural Substances and Environment, Chemistry Department, Faculty of Sciences and Techniques of Tangier, Tangier 90090, Morocco. FAU - Bouhrim, Mohamed AU - Bouhrim M AUID- ORCID: 0000-0001-9944-811X AD - Biological Engineering Laboratory, Faculty of Sciences and Techniques, Sultan Moulay Slimane University, Mghila, P.O. Box 523, Beni Mellal 23000, Morocco. FAU - Alqahtani, Moneerah J AU - Alqahtani MJ AUID- ORCID: 0000-0003-3013-1354 AD - Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia. FAU - Alqahtani, Jawaher H AU - Alqahtani JH AUID- ORCID: 0000-0002-3640-9612 AD - Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia. FAU - Miantezila Basilua, Joe AU - Miantezila Basilua J AUID- ORCID: 0000-0002-5658-9382 AD - BioSTM-UR 7537 Biostatistics, Processing and Modelling of Biological Data, Universite Paris Cite, 75006 Paris, France. FAU - Boulouiz, Aziz AU - Boulouiz A AD - Laboratory of Materials Chemistry and Applications (LCMA), Department of Chemistry, Faculty of Sciences, Mohammed Premier University, BP 717, Oujda 60000, Morocco. FAU - Touzani, Rachid AU - Touzani R AUID- ORCID: 0000-0001-7333-697X AD - Laboratory of Materials Chemistry and Applications (LCMA), Department of Chemistry, Faculty of Sciences, Mohammed Premier University, BP 717, Oujda 60000, Morocco. LA - eng GR - ORF-2026-583/King Saud University/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - EC 3.1.1.7 (Acetylcholinesterase) RN - 0 (Chromones) RN - 0 (Ligands) RN - 0 (Cholinesterase Inhibitors) RN - 8SSC91326P (Donepezil) SB - IM MH - *Acetylcholinesterase/chemistry/metabolism MH - *Chromones/chemistry/pharmacology MH - Molecular Docking Simulation MH - Humans MH - Ligands MH - *Cholinesterase Inhibitors/chemistry/pharmacology MH - Molecular Dynamics Simulation MH - Animals MH - Torpedo MH - Donepezil/chemistry MH - Protein Binding PMC - PMC13705499 OTO - NOTNLM OT - MM/PBSA OT - SDG3 OT - acetylcholinesterase OT - chromone derivatives OT - molecular docking OT - molecular dynamics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/15 CRDT- 2026/10/27 10:41 PHST- 2026/08/24 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ijms27209153 [pii] AID - ijms-27-09153 [pii] AID - 10.3390/ijms27209153 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 15;27(20):9153. doi: 10.3390/ijms27209153. PMID- 42941927 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 15 TI - Loss of Sidt2 in Mice Resembles Danon Disease and Results in Induction of a Type I Interferon Response with Increased Apoptosis. LID - 10.3390/ijms27209158 [doi] LID - 9158 AB - SIDT2 is a lysosomal membrane protein that-together with LAMP2-has been implicated in a recently described process known as RNAutophagy. Mutations in LAMP2 have long been known to cause Danon disease, a rare lysosomal storage disorder that affects multiple organ systems including brain and skeletal muscle. Recently, we have shown that loss of SIDT2 function in both humans and mice causes brain dysfunction, and it has been previously observed that loss of SIDT2 in mice also leads to muscle damage and reduced muscle strength. Here, we show that changes in the skeletal muscle of Sidt2(-/-) mice are reminiscent of those seen in Danon disease as evidenced by accumulation of autophagic vacuoles with sarcolemmal features, selective atrophy of fast twitch fibers, and a shared alteration in gene expression. Moreover, we find that loss of SIDT2 not only leads to impaired autophagy in skeletal muscle as previously described but also stimulates a type I interferon response and increased apoptosis. FAU - Barrios, Marilou H AU - Barrios MH AUID- ORCID: 0000-0002-1367-7163 AD - The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3050, Australia. AD - Department of Medical Biology, University of Melbourne, Parkville, VIC 3050, Australia. FAU - Nguyen, Tan A AU - Nguyen TA AUID- ORCID: 0000-0003-2696-9170 AD - The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3050, Australia. AD - Department of Medical Biology, University of Melbourne, Parkville, VIC 3050, Australia. FAU - Houweling, Peter J AU - Houweling PJ AUID- ORCID: 0000-0001-6132-2554 AD - Murdoch Children's Research Institute, Parkville, VIC 3052, Australia. AD - Department of Paediatrics, University of Melbourne, Parkville, VIC 3052, Australia. FAU - Frank, Daniel AU - Frank D AD - The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3050, Australia. FAU - Garton, Fleur C AU - Garton FC AD - Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia. FAU - Lidov, Hart G AU - Lidov HG AD - Department of Pathology, Boston Children's Hospital, Boston, MA 02115, USA. FAU - Tsui, Ellen AU - Tsui E AUID- ORCID: 0009-0005-7340-7412 AD - The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3050, Australia. AD - Department of Medical Biology, University of Melbourne, Parkville, VIC 3050, Australia. FAU - Garnham, Alexandra L AU - Garnham AL AD - The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3050, Australia. AD - Department of Medical Biology, University of Melbourne, Parkville, VIC 3050, Australia. FAU - Ambardekar, Amrut V AU - Ambardekar AV AUID- ORCID: 0000-0003-3503-4203 AD - Department of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA. FAU - Smyth, Gordon K AU - Smyth GK AUID- ORCID: 0000-0001-9221-2892 AD - The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3050, Australia. AD - Department of Medical Biology, University of Melbourne, Parkville, VIC 3050, Australia. FAU - Mestroni, Luisa AU - Mestroni L AUID- ORCID: 0000-0003-1116-2286 AD - Department of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA. FAU - Cleveland, Joseph C AU - Cleveland JC AD - Department of Surgery, Division of Cardiovascular Surgery, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA. FAU - Taylor, Matthew R G AU - Taylor MRG AUID- ORCID: 0000-0001-9043-0810 AD - Department of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA. FAU - North, Kathryn N AU - North KN AUID- ORCID: 0000-0003-0841-8009 AD - Murdoch Children's Research Institute, Parkville, VIC 3052, Australia. AD - Department of Paediatrics, University of Melbourne, Parkville, VIC 3052, Australia. FAU - Hunter, Craig P AU - Hunter CP AUID- ORCID: 0000-0002-1456-5657 AD - Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA. FAU - Pang, Ken C AU - Pang KC AUID- ORCID: 0000-0002-6881-775X AD - Murdoch Children's Research Institute, Parkville, VIC 3052, Australia. AD - Department of Paediatrics, University of Melbourne, Parkville, VIC 3052, Australia. AD - Royal Children's Hospital, Parkville, VIC 3052, Australia. LA - eng GR - UM1 TR004399/TR/NCATS NIH HHS/United States GR - TR004399/Colorado Clinical and Translational Sciences Institute/ GR - GNT1064591, GNT520574, and GNT2025645/National Health and Medical Research Council/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Interferon Type I) RN - 0 (Sidt2 protein, mouse) RN - 0 (Lysosomal Membrane Proteins) RN - 0 (Lysosomal-Associated Membrane Protein 2) RN - 0 (Nucleotide Transport Proteins) SB - IM MH - Animals MH - *Apoptosis/genetics MH - *Interferon Type I/metabolism MH - Mice MH - Autophagy MH - *Glycogen Storage Disease Type IIb/metabolism/genetics/pathology MH - Muscle, Skeletal/metabolism/pathology MH - Mice, Knockout MH - Lysosomal Membrane Proteins/genetics MH - Disease Models, Animal MH - Lysosomal-Associated Membrane Protein 2 MH - Nucleotide Transport Proteins PMC - PMC13705246 OTO - NOTNLM OT - Danon disease OT - RNAutophagy OT - Sidt2 OT - apoptosis OT - dsRNA OT - interferon response OT - linc-MD1 OT - muscle COIS- The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/15 CRDT- 2026/10/27 10:41 PHST- 2026/05/08 00:00 [received] PHST- 2026/09/04 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ijms27209158 [pii] AID - ijms-27-09158 [pii] AID - 10.3390/ijms27209158 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 15;27(20):9158. doi: 10.3390/ijms27209158. PMID- 42941925 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 15 TI - Identification of SOD1 Gene Variants in Mexican Patients with Amyotrophic Lateral Sclerosis. LID - 10.3390/ijms27209157 [doi] LID - 9157 AB - Amyotrophic Lateral Sclerosis (ALS) is a rapidly progressing, fatal neurodegenerative disorder that selectively affects motor neurons. Clinical manifestations include progressive muscle weakness, amyotrophy, fasciculations, dysphagia, and fatal respiratory failure. The median survival after symptom onset is typically 2 to 5 years. Despite its severity, the epidemiological profile and genetic landscape of ALS in Latin American populations, particularly in Mexico, remain unclear. In this study, we evaluated the mutational spectrum of the SOD1 gene in a Mexican cohort of 156 ALS patients. Using high-resolution melting (HRM) analysis and Sanger sequencing, we identified 22 SOD1 variants in 42 of 156 patients (overall variant carrier frequency, 26.92%). Pathogenic or likely pathogenic (P/LP) variants were found in 21 patients (13.5%). Notably, 5 of the 22 variants were novel, expanding the spectrum of SOD1 variants. The variant carrier frequency identified in this study is higher than that reported for European populations but similar to frequencies observed in Asian populations. Our results highlight the distinct genetic heterogeneity of ALS in Mexico and demonstrate that HRM is a rapid and effective method for identifying SOD1 gene variants. FAU - Gomez-Perez, Carlos Alberto AU - Gomez-Perez CA AD - Departamento de Genetica, Instituto Nacional de Neurologia y Neurocirugia, Ciudad de Mexico 14269, Mexico. AD - Posgrado en Ciencias Genomicas, Universidad Autonoma de la Ciudad de Mexico, Ciudad de Mexico 03104, Mexico. FAU - Ramirez-Garcia, Miguel Angel AU - Ramirez-Garcia MA AUID- ORCID: 0000-0003-3221-3636 AD - Departamento de Genetica, Instituto Nacional de Neurologia y Neurocirugia, Ciudad de Mexico 14269, Mexico. FAU - Ruiz-Sanchez, Elizabeth AU - Ruiz-Sanchez E AD - Laboratorio de Neuroquimica, Instituto Nacional de Neurologia y Neurocirugia, Ciudad de Mexico 14269, Mexico. FAU - Romero-Hidalgo, Sandra AU - Romero-Hidalgo S AD - Genomica Computacional, Instituto Nacional de Medicina Genomica, Ciudad de Mexico 14610, Mexico. FAU - Ochoa-Morales, Adriana AU - Ochoa-Morales A AD - Departamento de Genetica, Instituto Nacional de Neurologia y Neurocirugia, Ciudad de Mexico 14269, Mexico. FAU - Jara-Prado, Aurelio AU - Jara-Prado A AD - Departamento de Genetica, Instituto Nacional de Neurologia y Neurocirugia, Ciudad de Mexico 14269, Mexico. FAU - Mendez-Armenta, Marisela AU - Mendez-Armenta M AD - Laboratorio de Neuropatologia Experimental, Instituto Nacional de Neurologia y Neurocirugia, Ciudad de Mexico 14269, Mexico. FAU - Nava-Ruiz, Concepcion AU - Nava-Ruiz C AD - Laboratorio de Neuropatologia Experimental, Instituto Nacional de Neurologia y Neurocirugia, Ciudad de Mexico 14269, Mexico. FAU - Yescas-Gomez, Petra AU - Yescas-Gomez P AD - Departamento de Genetica, Instituto Nacional de Neurologia y Neurocirugia, Ciudad de Mexico 14269, Mexico. LA - eng GR - 262111/Secretariat of Science, Humanities, Technology, and Innovation (SECIHTI)/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - EC 1.15.1.1 (Superoxide Dismutase-1) RN - 0 (SOD1 protein, human) SB - IM MH - Humans MH - *Amyotrophic Lateral Sclerosis/genetics/epidemiology MH - *Superoxide Dismutase-1/genetics MH - Mexico/epidemiology MH - Female MH - Mutation MH - Male MH - Middle Aged MH - Aged MH - Gene Frequency MH - Genetic Variation MH - Adult PMC - PMC13705136 OTO - NOTNLM OT - Amyotrophic Lateral Sclerosis (ALS) OT - Mexico OT - SOD1 gene OT - genetic variants OT - high-resolution melting (HRM) OT - reactive oxygen species (ROS) COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/15 CRDT- 2026/10/27 10:41 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:41 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ijms27209157 [pii] AID - ijms-27-09157 [pii] AID - 10.3390/ijms27209157 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 15;27(20):9157. doi: 10.3390/ijms27209157. PMID- 42941924 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 15 TI - Amino Acid Profiles in Dried Blood Spots and Clinical Outcomes in Selective Fetal Growth Restriction: A Twin-Pair Study. LID - 10.3390/ijms27209156 [doi] LID - 9156 AB - Selective fetal growth restriction (sFGR) remains a leading cause of perinatal morbidity in multiple pregnancies; however, its pathogenesis is not yet fully elucidated. Investigating amino acid metabolism in weight-discordant twins may uncover critical metabolic pathways involved in this condition, given the essential roles of amino acids in fetal growth and energy supply. The use of dried blood spots (DBSs) enables the minimally invasive, retrospective evaluation of neonatal metabolic profiles, providing a unique opportunity to identify sFGR-associated signature and clarify differences between monochorionic (MC) and dichorionic (DC) placentation. In the present study, we profiled 23 amino acids in DBS from 23 twin pairs (11 discordant and 12 non-discordant, defined by postnatal birth-weight discordance), stratified by chorionicity, to identify specific metabolic signatures associated with sFGR and to evaluate their temporal dynamics and clinical significance. Our findings suggest that chorionicity is associated with distinct amino acid signatures of postnatal birth-weight discordance. In MC twins, the larger co-twin displayed significantly elevated glycine, alpha- and gamma-aminobutyric acids, serine, asparagine, and citrulline, alongside reduced proline and tryptophan. In DC twins, the larger co-twin exhibited higher alpha-aminobutyric acid, valine, leucine, arginine, and citrulline, whereas glycine was elevated in the smaller co-twin. Pathway enrichment analysis further suggested the enrichment of amino acid transport and protein synthesis pathways in MC postnatal discordance and nitric oxide (NO)-dependent signaling in DC postnatal discordance. Longitudinal follow-up showed a statistically significant attenuation of intrapair differences for several amino acids by the second time point. All 11 discordant pairs were retrospectively confirmed to fulfil the Delphi criteria for sFGR. These findings are exploratory and hypothesis-generating. FAU - Chagovets, Vitaliy V AU - Chagovets VV AD - National Medical Research Center for Obstetrics Gynecology and Perinatology Named After Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation, 117997 Moscow, Russia. FAU - Tokareva, Alisa O AU - Tokareva AO AD - National Medical Research Center for Obstetrics Gynecology and Perinatology Named After Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation, 117997 Moscow, Russia. FAU - Frankevich, Natalia A AU - Frankevich NA AD - National Medical Research Center for Obstetrics Gynecology and Perinatology Named After Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation, 117997 Moscow, Russia. FAU - Novoselova, Anastasia V AU - Novoselova AV AD - National Medical Research Center for Obstetrics Gynecology and Perinatology Named After Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation, 117997 Moscow, Russia. FAU - Yushina, Marina N AU - Yushina MN AD - National Medical Research Center for Obstetrics Gynecology and Perinatology Named After Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation, 117997 Moscow, Russia. FAU - Hajjar, Fadi AU - Hajjar F AD - National Medical Research Center for Obstetrics Gynecology and Perinatology Named After Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation, 117997 Moscow, Russia. FAU - Eldarov, Chupalav M AU - Eldarov CM AD - National Medical Research Center for Obstetrics Gynecology and Perinatology Named After Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation, 117997 Moscow, Russia. FAU - Michailova, Olga V AU - Michailova OV AD - National Medical Research Center for Obstetrics Gynecology and Perinatology Named After Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation, 117997 Moscow, Russia. FAU - Karapetyan, Tamara E AU - Karapetyan TE AD - National Medical Research Center for Obstetrics Gynecology and Perinatology Named After Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation, 117997 Moscow, Russia. FAU - Ryumina, Irina I AU - Ryumina II AD - National Medical Research Center for Obstetrics Gynecology and Perinatology Named After Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation, 117997 Moscow, Russia. FAU - Frankevich, Vladimir E AU - Frankevich VE AD - National Medical Research Center for Obstetrics Gynecology and Perinatology Named After Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation, 117997 Moscow, Russia. FAU - Sukhikh, Gennady T AU - Sukhikh GT AD - National Medical Research Center for Obstetrics Gynecology and Perinatology Named After Academician V.I. Kulakov of the Ministry of Healthcare of Russian Federation, 117997 Moscow, Russia. AD - Department of Obstetrics, Gynecology, Perinatology and Reproductology, Institute of Professional Education, Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation, 119991 Moscow, Russia. LA - eng GR - 125050605833-4/Ministry of Health of the Russian Federation/ PT - Journal Article PT - Twin Study DEP - 20261015 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Amino Acids) SB - IM MH - Humans MH - *Amino Acids/blood/metabolism MH - Female MH - *Fetal Growth Retardation/blood/metabolism/diagnosis MH - Pregnancy MH - *Dried Blood Spot Testing/methods MH - Infant, Newborn MH - Birth Weight MH - Male PMC - PMC13705542 OTO - NOTNLM OT - amino acids OT - dichorionic twins OT - dried blood spots OT - mass spectrometry OT - monochorionic twins OT - neonatal outcomes OT - over-representation analysis OT - selective fetal growth restriction COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/15 CRDT- 2026/10/27 10:40 PHST- 2026/08/18 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ijms27209156 [pii] AID - ijms-27-09156 [pii] AID - 10.3390/ijms27209156 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 15;27(20):9156. doi: 10.3390/ijms27209156. PMID- 42941913 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 15 TI - Concentration-Associated DNA Methylation Remodeling of Oncogenic Signaling Networks Following Moringa oleifera Treatment in Metastatic PC3 Prostate Cancer Cells. LID - 10.3390/ijms27209144 [doi] LID - 9144 AB - PC3 prostate cancer (PCa) cells exhibit high metastatic potential, androgen independence, and activation of multiple oncogenic signaling pathways. Although Moringa oleifera has recognized anticancer properties, its effects on DNA methylation in aggressive PCa remain largely unexplored. Here, PC3 cells were treated with Moringa oleifera extract (MO) at 100 or 200 mug/mL and evaluated using functional and molecular analyses alongside genome-wide DNA methylation profiling. Both concentrations reduced AKT phosphorylation and the expression of mesenchymal and invasion-associated markers, whereas increased apoptosis was observed at 200 mug/mL MO. ERK1/2 phosphorylation differed between the two concentrations but was not significantly reduced relative to untreated controls. Methylation profiling revealed concentration-specific pathway-enrichment profiles among genes associated with differentially methylated regions (DMRs). At 100 mug/mL, hypermethylated DMR-associated genes were enriched mainly in small GTPase signaling, focal adhesion, cytoskeletal organization, axon guidance, MAPK, Rap1, and RHO signaling. At 200 mug/mL, enrichment involved cell adhesion, Wnt signaling, RAC1/CDC42 GTPase cycles, and VEGF/VEGFR2-mediated signaling. Hypomethylated DMR-associated genes showed distinct but partly overlapping enrichment profiles. In the direct comparison, DMRs with higher methylation at 100 mug/mL were associated with axon guidance, focal adhesion, actin cytoskeleton regulation, MAPK, and EPH-Ephrin signaling, whereas those with higher methylation at 200 mug/mL were associated mainly with NOTCH and RHOB/RHOC signaling. These findings identify concentration-specific DNA methylation changes in genes belonging to networks relevant to aggressive PCa, accompanied by molecular and cellular responses, without establishing a causal relationship between methylation changes and those responses. FAU - Mancuso, Francesca AU - Mancuso F AUID- ORCID: 0000-0001-6562-5923 AD - Department of Medicine and Surgery, University of Perugia, 06132 Perugia, Italy. FAU - Lilli, Cinzia AU - Lilli C AD - Department of Medicine and Surgery, University of Perugia, 06132 Perugia, Italy. FAU - Bellucci, Catia AU - Bellucci C AD - Department of Medicine and Surgery, University of Perugia, 06132 Perugia, Italy. FAU - Ceccarelli, Veronica AU - Ceccarelli V AUID- ORCID: 0000-0003-0424-6940 AD - Department of Medicine and Surgery, University of Perugia, 06132 Perugia, Italy. FAU - Stabile, Anna Maria AU - Stabile AM AUID- ORCID: 0000-0003-1047-4210 AD - Department of Medicine and Surgery, University of Perugia, 06132 Perugia, Italy. FAU - Gambelunghe, Cristiana AU - Gambelunghe C AUID- ORCID: 0000-0003-2045-9145 AD - Department of Medicine and Surgery, University of Perugia, 06132 Perugia, Italy. FAU - Pugliese, Ludovica AU - Pugliese L AD - Department of Medicine and Surgery, University of Perugia, 06132 Perugia, Italy. FAU - Cecchetti, Margherita AU - Cecchetti M AUID- ORCID: 0009-0008-0091-7042 AD - Cluster of Excellence on Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50931 Cologne, Germany. FAU - Antognelli, Cinzia AU - Antognelli C AUID- ORCID: 0000-0002-8259-680X AD - Department of Medicine and Surgery, University of Perugia, 06132 Perugia, Italy. FAU - Baroni, Tiziano AU - Baroni T AD - Department of Medicine and Surgery, University of Perugia, 06132 Perugia, Italy. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Plant Extracts) SB - IM MH - *DNA Methylation/drug effects MH - Humans MH - *Prostatic Neoplasms/genetics/drug therapy/pathology/metabolism MH - Male MH - *Moringa oleifera/chemistry MH - *Signal Transduction/drug effects MH - PC-3 Cells MH - Gene Expression Regulation, Neoplastic/drug effects MH - *Plant Extracts/pharmacology PMC - PMC13705501 OTO - NOTNLM OT - DNA methylation OT - EMT OT - MAPK OT - Moringa oleifera OT - PC3 OT - PI3K-AKT OT - angiogenesis OT - epigenetics OT - prostate cancer COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/15 CRDT- 2026/10/27 10:40 PHST- 2026/08/10 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ijms27209144 [pii] AID - ijms-27-09144 [pii] AID - 10.3390/ijms27209144 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 15;27(20):9144. doi: 10.3390/ijms27209144. PMID- 42941895 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 15 TI - Lymphatic Preconditioning for Preservation of Lymphatic Function. LID - 10.3390/ijms27209125 [doi] LID - 9125 AB - Lymphedema is chronic swelling from lymphatic dysfunction. The vascular delay phenomenon is used in flap reconstruction to increase flap viability through staged selective partial disruption of blood supply to enhance flap survival, followed by flap transfer 7-10 days later. A similar corollary for lymphatics remains unknown. The purpose of this study was to evaluate whether lymphatic preconditioning with staged disruption of lymphatics in the murine hindlimb lymphedema model can be protective against lymphedema. C57BL/6 mice underwent 20 Gy hindlimb irradiation. Group 1 (immediate lymphatic disruption) underwent a circumferential skin incision, complete lymphatic disruption, and popliteal lymph node excision in a single-stage operation. Group 2 (delayed lymphatic disruption) underwent a semi-circumferential inguinal skin incision and dominant lymphatic transection and, 7 days later, received a circumferential skin incision, complete lymphatic transection, and popliteal lymphadenectomy. Paw thickness was measured weekly. Near-infrared laser lymphangiography and immunohistochemistry were performed on D35. Group 2 had a lower average paw thickness (p = 0.0008) and greater lymphatic clearance signal intensity at 24 h (p = 0.0035). Immunohistochemistry indicated an abundance of the lymphatic endothelial-specific marker podoplanin in Group 2 compared to Group 1 (p = 0.03). This study demonstrates evidence for a novel concept of a lymphatic delay phenomenon parallel to the well-known vascular delay phenomenon. FAU - Ahmed, Shahnur AU - Ahmed S AUID- ORCID: 0000-0001-8707-9230 AD - Division of Plastic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA. FAU - Mohan, Ganesh AU - Mohan G AUID- ORCID: 0000-0003-4636-1250 AD - Division of Plastic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA. FAU - Sidhu, Angad S AU - Sidhu AS AUID- ORCID: 0009-0007-9825-8964 AD - Division of Plastic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA. FAU - Konig, Daniel J AU - Konig DJ AUID- ORCID: 0009-0007-6612-6908 AD - Division of Plastic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA. FAU - Subi-Kasozi, Christopher A AU - Subi-Kasozi CA AUID- ORCID: 0009-0007-0757-7186 AD - Division of Plastic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA. FAU - Sullivan, Steven AU - Sullivan S AD - Division of Plastic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA. FAU - Jorge, Miguel D AU - Jorge MD AD - Division of Plastic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA. FAU - Calabrese, Kristen N AU - Calabrese KN AUID- ORCID: 0009-0004-2574-7300 AD - Division of Plastic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA. FAU - Sinex, Helen C AU - Sinex HC AD - Department of Radiation Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USA. FAU - Garrett, Joy AU - Garrett J AD - Department of Radiation Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USA. FAU - Dynlacht, Joseph R AU - Dynlacht JR AD - Department of Radiation Medicine, Indiana University School of Medicine, Indianapolis, IN 46202, USA. FAU - Sinha, Mithun AU - Sinha M AUID- ORCID: 0000-0002-9222-1921 AD - Division of Plastic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA. FAU - Hassanein, Aladdin H AU - Hassanein AH AUID- ORCID: 0000-0002-0966-7851 AD - Division of Plastic Surgery, Indiana University School of Medicine, Indianapolis, IN 46202, USA. LA - eng GR - K08 HL167164/HL/NHLBI NIH HHS/United States GR - 1R01HL180721 (AHH)/National Heart Lung and Blood Institute/ GR - R21AR082600 (AHH)/National Heart Lung and Blood Institute/ GR - R21 AR082600/AR/NIAMS NIH HHS/United States GR - to AHH/Plastic Surgery Foundation/ GR - to AHH/Indiana Clinical and Translational Sciences Institute/ GR - K08HL167164 (AHH)/National Heart Lung and Blood Institute/ GR - L40 HL175936/HL/NHLBI NIH HHS/United States GR - L40HL175936 to SA/NIH LRP/ GR - R01 HL180721/HL/NHLBI NIH HHS/United States PT - Journal Article DEP - 20261015 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - Animals MH - *Lymphedema/prevention & control/etiology/pathology/physiopathology MH - Mice MH - Mice, Inbred C57BL MH - *Lymphatic Vessels MH - Hindlimb MH - Lymph Node Excision MH - Lymphography MH - Disease Models, Animal MH - Lymph Nodes MH - Male MH - *Lymphatic System PMC - PMC13704935 OTO - NOTNLM OT - lymphadenectomy OT - lymphedema OT - murine hindlimb OT - negative pressure therapy OT - prevention OT - radiation COIS- The authors declare that they have no competing interests. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/15 CRDT- 2026/10/27 10:40 PHST- 2026/08/18 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ijms27209125 [pii] AID - ijms-27-09125 [pii] AID - 10.3390/ijms27209125 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 15;27(20):9125. doi: 10.3390/ijms27209125. PMID- 42941892 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 15 TI - Exploratory Analysis of Selected MicroRNAs as Tissue-Based Candidate Biomarkers in Ovarian Cancer. LID - 10.3390/ijms27209122 [doi] LID - 9122 AB - The aim of the study was to evaluate the expression and exploratory diagnostic potential of selected microRNAs (miRNAs) in ovarian cancer tissue compared with benign ovarian lesions. Tissue samples from 24 patients with epithelial ovarian cancer and 22 patients with benign ovarian lesions were analyzed by quantitative real-time polymerase chain reaction. Ten candidate miRNAs were assessed; six met the predefined detection criteria and were retained for analysis. Group differences were tested with non-parametric methods and false discovery rate (FDR) correction. Diagnostic performance was evaluated by receiver operating characteristic (ROC) analysis, and multivariable logistic regression adjusted for age and menopausal status. Survival analyses were exploratory. miR-381-3p was downregulated, whereas miR-582-5p and miR-425-5p were upregulated in ovarian cancer compared with benign ovarian lesions; all three differences remained significant after FDR correction. miR-425-5p showed the highest individual diagnostic accuracy (AUC = 0.854) and remained associated with ovarian cancer after adjustment for age and menopausal status (OR = 7.84; 95% CI = 2.05-29.97; p = 0.003). This association was also retained in Firth penalized logistic regression. An exploratory three-miRNA panel achieved an apparent AUC of 0.858 but did not significantly outperform miR-425-5p alone (DeLong p = 0.596); its optimism-corrected bootstrap AUC was 0.822. No significant associations with survival were identified. miR-425-5p was the strongest candidate biomarker in this exploratory tissue-based study. Independent validation is required before clinical application. FAU - Kozlowski, Mateusz AU - Kozlowski M AUID- ORCID: 0000-0002-1176-2153 AD - Department of Gynecological Surgery and Gynecological Oncology of Adults and Adolescents, Pomeranian Medical University in Szczecin, Al. Powstancow Wielkopolskich 72, 70-111 Szczecin, Poland. FAU - Borzyszkowska, Dominika AU - Borzyszkowska D AUID- ORCID: 0000-0001-6259-5052 AD - Department of Pediatric Surgery, Orthopedics and Urology with a Subdivision of the Burn Treatment, Independent Public Specialist Healthcare Facility "Zdroje", 70-780 Szczecin, Poland. FAU - Lubkowski, Michal AU - Lubkowski M AUID- ORCID: 0009-0007-4600-8920 AD - Department of Functional Anatomy, Pomeranian Medical University in Szczecin, Al. Powstancow Wielkopolskich 72, 70-111 Szczecin, Poland. FAU - Komaniecka, Nina AU - Komaniecka N AUID- ORCID: 0009-0006-3162-4870 AD - Department of Experimental and Clinical Pharmacology, Pomeranian Medical University in Szczecin, Al. Powstancow Wielkopolskich 72, 70-111 Szczecin, Poland. FAU - Mikulka, Aleksandra AU - Mikulka A AUID- ORCID: 0009-0007-9687-0308 AD - Department of Gynecologic Oncology and Reconstructive Gynecology, Department of Obstetrics and Gynecology with the High-Risk Pregnancy Unit, Independent Public Specialist Healthcare Facility "Zdroje", 70-780 Szczecin, Poland. FAU - Birger, Radoslaw AU - Birger R AUID- ORCID: 0009-0008-6256-5695 AD - Laboratory of Pharmacodynamics, Pomeranian Medical University in Szczecin, Plac Polskiego Czerwonego Krzyza 1, 71-244 Szczecin, Poland. FAU - Kolczewski, Piotr AU - Kolczewski P AUID- ORCID: 0000-0002-0855-6471 AD - Private Clinic Esthegyn, ul. Mazurska 7, 70-424 Szczecin, Poland. FAU - Brodowska, Agnieszka AU - Brodowska A AUID- ORCID: 0000-0002-6425-1648 AD - Department of Gynecology, Endocrinology and Gynecological Oncology, Pomeranian Medical University in Szczecin, Unii Lubelskiej 1, 71-252 Szczecin, Poland. FAU - Kurzawski, Mateusz AU - Kurzawski M AUID- ORCID: 0000-0002-4293-7788 AD - Laboratory of Pharmacodynamics, Pomeranian Medical University in Szczecin, Plac Polskiego Czerwonego Krzyza 1, 71-244 Szczecin, Poland. FAU - Kempinska-Podhorodecka, Agnieszka AU - Kempinska-Podhorodecka A AUID- ORCID: 0000-0002-7513-8640 AD - Department of Functional Anatomy, Pomeranian Medical University in Szczecin, Al. Powstancow Wielkopolskich 72, 70-111 Szczecin, Poland. FAU - Cymbaluk-Ploska, Aneta AU - Cymbaluk-Ploska A AD - Department of Gynecological Surgery and Gynecological Oncology of Adults and Adolescents, Pomeranian Medical University in Szczecin, Al. Powstancow Wielkopolskich 72, 70-111 Szczecin, Poland. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (MicroRNAs) RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - Female MH - *MicroRNAs/genetics MH - *Ovarian Neoplasms/genetics/diagnosis/pathology MH - *Biomarkers, Tumor/genetics MH - Gene Expression Regulation, Neoplastic MH - Middle Aged MH - ROC Curve MH - Adult MH - Aged MH - Prognosis PMC - PMC13705106 OTO - NOTNLM OT - ROC curve OT - biomarkers OT - diagnosis OT - gene expression OT - miR-381-3p OT - miR-425-5p OT - miR-582-5p OT - microRNAs OT - ovarian cancer OT - survival analysis COIS- Dr. Piotr Kolczewski is affiliated with Private Clinic Esthegyn, which constitutes his private medical practice. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/15 CRDT- 2026/10/27 10:40 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ijms27209122 [pii] AID - ijms-27-09122 [pii] AID - 10.3390/ijms27209122 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 15;27(20):9122. doi: 10.3390/ijms27209122. PMID- 42941890 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 14 TI - Genome-Wide Characterization of the LRX Gene Family in Three Cotton Species and Preliminary Evidence Linking GhLRX2/GhLRX19 to Plant Height in Upland Cotton. LID - 10.3390/ijms27209119 [doi] LID - 9119 AB - Plant height is an important agronomic trait affecting cotton architecture and mechanized production. Leucine-rich repeat extensins (LRXs) are cell-wall-associated proteins involved in cell-wall organization, cell elongation, and cell-wall integrity signaling. In this study, genome-wide analysis of Gossypium hirsutum, G. arboreum, and G. raimondii identified 74 LRX loci, comprising 36 GhLRX, 19 GaLRX, and 19 GrLRX genes. These 74 loci were represented by 79 annotated protein models, including five additional models from three GrLRX loci. Maximum-likelihood phylogenetic analysis of 74 representative cotton proteins and 11 Arabidopsis proteins assigned them to four descriptive groups. Of the 79 protein models, 75 were predicted to contain Sec/SPI-type signal peptides. Conserved motif and gene structure analyses revealed broadly similar organization with variation among members and alternative isoforms. Promoter analysis identified putative hormone- and stress-related cis-acting elements. Collinearity analyses revealed syntenic relationships within upland cotton and with its diploid relatives. Public transcriptome data showed variation in GhLRX transcript abundance across tissues, developmental stages, and abiotic stress conditions. The homoeologous genes GhLRX2 and GhLRX19 showed relatively high transcript abundance in stems and 20-day-post-anthesis fibers and were selected for preliminary investigation. Virus-induced gene silencing (VIGS) treatment targeting a sequence shared by GhLRX2 and GhLRX19 was associated with lower combined transcript abundance and reduced plant height. These findings provide preliminary evidence linking this shared-sequence VIGS treatment to reduced plant height, while the individual responses and functions of the two homoeologs remain unresolved. FAU - Li, Jie AU - Li J AD - College of Agriculture, Shihezi University, Shihezi 832000, China. FAU - Ma, Xiaohu AU - Ma X AD - College of Agriculture, Shihezi University, Shihezi 832000, China. FAU - Si, Shihua AU - Si S AD - College of Agriculture, Shihezi University, Shihezi 832000, China. FAU - Weng, Tianyu AU - Weng T AD - College of Agriculture, Shihezi University, Shihezi 832000, China. FAU - Chen, Shuaishuai AU - Chen S AD - College of Agriculture, Shihezi University, Shihezi 832000, China. FAU - Zhang, Xinyu AU - Zhang X AD - College of Agriculture, Shihezi University, Shihezi 832000, China. FAU - Ren, Shen AU - Ren S AD - College of Agriculture, Shihezi University, Shihezi 832000, China. FAU - Liu, Feng AU - Liu F AUID- ORCID: 0000-0002-4693-8489 AD - College of Agriculture, Shihezi University, Shihezi 832000, China. FAU - Li, Yanjun AU - Li Y AD - College of Agriculture, Shihezi University, Shihezi 832000, China. FAU - Xue, Fei AU - Xue F AUID- ORCID: 0000-0001-6311-1961 AD - College of Agriculture, Shihezi University, Shihezi 832000, China. FAU - Xuan, Lizhong AU - Xuan L AD - Agricultural Technology Extension Station, Xinjiang Production and Construction Corps, Urumqi 830011, China. FAU - Sun, Jie AU - Sun J AUID- ORCID: 0000-0002-8068-8102 AD - College of Agriculture, Shihezi University, Shihezi 832000, China. LA - eng GR - 2023AA008/Science and Technology Major Program of BINGTUAN/ GR - 2025DA001/Bingtuan Science and Technology Program of China/ GR - XJARS03-19/earmarked fund for XJARS/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Plant Proteins) RN - 0 (Leucine-Rich Repeat Proteins) SB - IM MH - *Gossypium/genetics/growth & development MH - *Plant Proteins/genetics/metabolism MH - Phylogeny MH - *Multigene Family MH - Gene Expression Regulation, Plant MH - Genome, Plant MH - Promoter Regions, Genetic MH - Leucine-Rich Repeat Proteins MH - Genome-Wide Association Study PMC - PMC13705520 OTO - NOTNLM OT - Gossypium hirsutum OT - genome-wide identification OT - leucine-rich repeat extensin OT - plant height OT - virus-induced gene silencing COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/14 CRDT- 2026/10/27 10:40 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - ijms27209119 [pii] AID - ijms-27-09119 [pii] AID - 10.3390/ijms27209119 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 14;27(20):9119. doi: 10.3390/ijms27209119. PMID- 42941887 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 14 TI - Molecular Regulators for the Wnt/beta-Catenin Signalling Pathway for Bone Remodelling in Periodontitis: A Scoping Review. LID - 10.3390/ijms27209117 [doi] LID - 9117 AB - Periodontitis is a chronic inflammatory disease that destroys the periodontal ligament, leading to alveolar bone loss and ultimately tooth loss. The Wnt/beta-catenin signalling pathway is an important molecular signalling pathway that controls the expression of bone remodelling-related genes. Manipulating this pathway is a recurring theme in recent studies, suggesting a viable therapeutic target. A scoping review was conducted according to PRISMA guidelines to identify the latest molecular regulators used in manipulating the signalling pathway between 2007 and 2025 from databases such as Web of Science, Ovid Medline, and Scopus. Only primary articles relevant to the signalling pathway, periodontitis, and bone homeostasis were selected. A total of 22 articles were identified, using various molecules ranging from plant extracts such as field horsetail to pre-existing drugs. The molecules function by altering the activities of targets within the signalling pathway such as beta-catenin and GSK-3beta phosphorylation, ultimately increasing osteoblast differentiation for bone growth that was reduced in periodontitis cases. The Wnt/beta-catenin signalling pathway is a favoured target for potential therapeutic interventions for periodontitis. However, most studies are limited to in vitro methods and requires further description on the relationships between periodontitis and the signalling pathway. FAU - Amir Maeraj, Hasif AU - Amir Maeraj H AD - Department of Craniofacial Diagnostics and Biosciences, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia. FAU - Rajoo, Shivaroobini AU - Rajoo S AD - Department of Restorative Dentistry, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia. FAU - Mohd-Said, Shahida AU - Mohd-Said S AUID- ORCID: 0000-0003-4387-1087 AD - Department of Restorative Dentistry, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia. FAU - Nasruddin, Nurrul Shaqinah AU - Nasruddin NS AD - Department of Craniofacial Diagnostics and Biosciences, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia. FAU - Taib, Izatus Shima AU - Taib IS AUID- ORCID: 0000-0001-9901-766X AD - Centre for Diagnostics, Therapeutics, and Investigative Studies, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia. FAU - Wong, Sok Kuan AU - Wong SK AUID- ORCID: 0000-0003-1184-4551 AD - Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur 56000, Malaysia. FAU - Jayusman, Putri Ayu AU - Jayusman PA AUID- ORCID: 0000-0001-9742-160X AD - Department of Craniofacial Diagnostics and Biosciences, Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, Malaysia. LA - eng GR - FRGS/1/2024/SKK10/UKM/02/9/Ministry of Higher Education/ PT - Journal Article PT - Review PT - Scoping Review DEP - 20261014 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (beta Catenin) RN - EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta) SB - IM MH - Humans MH - *Wnt Signaling Pathway/drug effects MH - *Periodontitis/metabolism/pathology/drug therapy MH - *Bone Remodeling MH - Animals MH - *beta Catenin/metabolism MH - Glycogen Synthase Kinase 3 beta/metabolism PMC - PMC13705578 OTO - NOTNLM OT - Wnt/beta-catenin signalling pathway OT - bone remodelling OT - periodontitis COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analysis, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/14 CRDT- 2026/10/27 10:40 PHST- 2026/09/12 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - ijms27209117 [pii] AID - ijms-27-09117 [pii] AID - 10.3390/ijms27209117 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 14;27(20):9117. doi: 10.3390/ijms27209117. PMID- 42941882 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 14 TI - Azithromycin Derivatives for Overcoming Antibacterial Resistance: Structural Modification and Structure-Activity Relationships. LID - 10.3390/ijms27209110 [doi] LID - 9110 AB - Antimicrobial resistance has significantly compromised the therapeutic efficacy of many antibiotics, highlighting the need for new agents capable of overcoming bacterial resistance. The discovery of antibiotics in the early 20th century contributed significantly to transforming modern medicine and saved countless lives. However, the alarming increase in antibiotic resistance now threatens these achievements. The misuse and overuse of antibiotics have driven the continuous evolution and adaptation of microorganisms, contributing to this growing global health challenge. Azithromycin (AZM), a widely used macrolide antibiotic with favorable pharmacokinetic properties, is increasingly affected by resistance mechanisms. A number of researchers have therefore designed and developed AZM derivatives with promising antibacterial properties. This review summarizes advances in the structural modification of AZM, focusing on side-chain modification, heterocyclic substitution, molecular hybridization, and their associated structure-activity relationships. Selected derivatives demonstrated enhanced activity against resistant bacterial pathogens compared with AZM, although activity varies according to substituent position, linker structure, and overall molecular properties. Nevertheless, the activity of many AZM derivatives against Gram-negative organisms remains limited, with restricted outer-membrane permeability and inadequate intracellular accumulation representing important barriers to achieving effective antibacterial activity. Overall, the structural modification of AZM represents a promising strategy for developing potential antibacterial agents, but further pharmacological and in vivo studies are required to establish their therapeutic potential. FAU - Ngece, Kwanele AU - Ngece K AUID- ORCID: 0000-0002-3955-1083 AD - Chemistry Discipline, Department of Chemical and Earth Sciences, Faculty of Science and Agriculture, University of Fort Hare, Alice 5700, South Africa. FAU - Paca, Athandwe M AU - Paca AM AD - Chemistry Discipline, Department of Chemical and Earth Sciences, Faculty of Science and Agriculture, University of Fort Hare, Alice 5700, South Africa. FAU - Buwa-Komoreng, Lisa V AU - Buwa-Komoreng LV AUID- ORCID: 0000-0002-1592-9459 AD - Infectious Diseases and Medicinal Plants Research Niche Area, Botany Discipline, Department of Biotechnology and Biological Sciences, Faculty of Science and Agriculture, University of Fort Hare, Alice 5700, South Africa. FAU - Aderibigbe, Blessings A AU - Aderibigbe BA AD - Department of Chemistry, Physics, and Geosciences, Tarleton State University, Stephenville, TX 76402, USA. FAU - Khwaza, Vuyolwethu AU - Khwaza V AUID- ORCID: 0000-0003-4875-1535 AD - Chemistry Discipline, Department of Chemical and Earth Sciences, Faculty of Science and Agriculture, University of Fort Hare, Alice 5700, South Africa. AD - Infectious Diseases and Medicinal Plants Research Niche Area, Botany Discipline, Department of Biotechnology and Biological Sciences, Faculty of Science and Agriculture, University of Fort Hare, Alice 5700, South Africa. LA - eng GR - RCMPD240710243305/National Research Foundation/ GR - SAMRC/South African Medical Research Council/ PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 83905-01-5 (Azithromycin) RN - 0 (Anti-Bacterial Agents) SB - IM MH - Structure-Activity Relationship MH - *Azithromycin/pharmacology/chemistry/analogs & derivatives MH - *Anti-Bacterial Agents/pharmacology/chemistry MH - *Drug Resistance, Bacterial/drug effects MH - Humans MH - Microbial Sensitivity Tests MH - Animals MH - Bacteria/drug effects MH - Gram-Negative Bacteria/drug effects PMC - PMC13705085 OTO - NOTNLM OT - antibacterial agents OT - antimicrobial resistance OT - azithromycin OT - azithromycin derivatives OT - molecular hybridization OT - structural modification OT - structure-activity relationship COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/14 CRDT- 2026/10/27 10:40 PHST- 2026/08/26 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - ijms27209110 [pii] AID - ijms-27-09110 [pii] AID - 10.3390/ijms27209110 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 14;27(20):9110. doi: 10.3390/ijms27209110. PMID- 42941873 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 14 TI - Peptides and Peptide Analogues Targeting Acetylcholinesterase-Associated Mechanisms in Alzheimer's Disease and Dementias: Evidence Gaps, Design Strategies, and Translational Perspectives. LID - 10.3390/ijms27209094 [doi] LID - 9094 AB - Alzheimer's disease and related dementias involve interacting disturbances in amyloid-beta, tau, neuroinflammation, oxidative stress, mitochondrial function, synaptic integrity, and cholinergic neurotransmission. Acetylcholinesterase, in addition to terminating cholinergic signalling through acetylcholine hydrolysis, participates in non-catalytic interactions associated with amyloid-beta, providing a mechanistic rationale for multi-target therapeutic strategies. This critical narrative review evaluates natural acetylcholinesterase-inhibitory peptides, amyloid-targeting sequences, D-enantiomeric and retro-inverso analogues, endogenous and neuroprotective peptides, and selected peptidomimetic and multifunctional designs. The literature is considered according to an evidence hierarchy that distinguishes direct acetylcholinesterase/peripheral anionic site target engagement from independently demonstrated secondary mechanisms, cellular activity, central nervous system exposure, in vivo pharmacodynamics, and clinical efficacy. The reviewed evidence indicates that peptides and peptide-derived compounds offer substantial molecular recognition and structural adaptability and may, in principle, be engineered to combine selected cholinergic and disease-relevant functions. Importantly, no single candidate reviewed here has experimentally demonstrated all of the proposed cholinergic, anti-amyloid, anti-inflammatory, antioxidant, mitochondrial, and synaptoprotective activities simultaneously. However, neuroprotective, anti-amyloid, or cognitive effects alone do not establish direct acetylcholinesterase modulation, and many proposed multifunctional candidates lack experimental evidence of acetylcholinesterase/peripheral anionic site engagement. Overall, the available literature supports the feasibility of designing multifunctional peptide-based acetylcholinesterase-directed agents, but the evidence remains insufficient to establish a clinically validated class of multifunctional acetylcholinesterase modulators. FAU - Zdunek, Joanna AU - Zdunek J AD - Department of Biochemistry and Pharmacogenomics, Medical University of Warsaw, 1 Banacha Str., 02-097 Warsaw, Poland. FAU - Grodner, Blazej AU - Grodner B AUID- ORCID: 0000-0003-2184-6796 AD - Department of Biochemistry and Pharmacogenomics, Medical University of Warsaw, 1 Banacha Str., 02-097 Warsaw, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - EC 3.1.1.7 (Acetylcholinesterase) RN - 0 (Cholinesterase Inhibitors) RN - 0 (Peptides) RN - 0 (Neuroprotective Agents) RN - 0 (Amyloid beta-Peptides) SB - IM MH - Humans MH - *Alzheimer Disease/drug therapy/metabolism MH - *Acetylcholinesterase/metabolism MH - Animals MH - *Cholinesterase Inhibitors/therapeutic use/pharmacology/chemistry MH - *Peptides/therapeutic use/chemistry/pharmacology MH - *Dementia/drug therapy/metabolism MH - Neuroprotective Agents/therapeutic use/pharmacology/chemistry MH - Amyloid beta-Peptides/metabolism MH - Drug Design PMC - PMC13705407 OTO - NOTNLM OT - Alzheimer's disease OT - acetylcholinesterase OT - amyloid-beta OT - blood-brain barrier OT - cholinergic system OT - multi-target-directed ligands OT - neuroprotection OT - peptides OT - peptidomimetics OT - target engagement COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/14 CRDT- 2026/10/27 10:40 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - ijms27209094 [pii] AID - ijms-27-09094 [pii] AID - 10.3390/ijms27209094 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 14;27(20):9094. doi: 10.3390/ijms27209094. PMID- 42941866 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 14 TI - Endothelial Redox Dysregulation in Pulmonary Arterial Hypertension: Compartmentalized Mechanisms and Therapeutic Implications. LID - 10.3390/ijms27209096 [doi] LID - 9096 AB - Pulmonary arterial hypertension (PAH) is a progressive pulmonary vasculopathy in which endothelial dysfunction drives increased pulmonary vascular resistance and arterial remodeling. Redox signaling is central to endothelial homeostasis, and its disruption in PAH reflects changes in the magnitude, source, and subcellular location of reactive oxygen species (ROS) production, as well as the molecular targets they engage. In healthy endothelium, compartmentalized redox signaling supports adaptive responses while preserving nitric oxide bioavailability. Loss of this spatial control in PAH can convert normally transient redox signals into persistent endothelial dysfunction, although the strength of evidence for this conversion differs substantially between pathways. This review examines how disease-associated stressors reshape endothelial redox signaling through NADPH oxidases, mitochondria, eNOS, iron-dependent chemistry, and nuclear pathways and how these compartments communicate. We also examine emerging circulating markers of redox and endothelial dysfunction and evaluate redox-directed therapies across experimental pulmonary hypertension and PAH. Throughout, we grade each mechanism according to whether it is supported by evidence from human PAH vascular tissue, patient-derived pulmonary arterial endothelial cells, experimental pulmonary hypertension models, and pulmonary endothelial models or by extrapolation from other relevant disease systems, and we identify where compartment-specific evidence is inferred from enzyme localization rather than measured directly. Current evidence supports moving beyond broad antioxidant approaches toward strategies that selectively target pathogenic redox signaling within the relevant endothelial compartment. FAU - Moreno-Caceres, Santiago AU - Moreno-Caceres S AUID- ORCID: 0009-0000-9698-2835 AD - Center for Translational Science, Florida International University, Port Saint Lucie, FL 34987, USA. AD - Department of Cellular & Molecular Medicine, Herbert Wertheim College of Medicine, Florida International University, Miami, FL 33199, USA. FAU - Marciano, David P AU - Marciano DP AUID- ORCID: 0000-0001-8868-2138 AD - Center for Translational Science, Florida International University, Port Saint Lucie, FL 34987, USA. AD - Department of Cellular & Molecular Medicine, Herbert Wertheim College of Medicine, Florida International University, Miami, FL 33199, USA. LA - eng GR - R00 HL145097/HL/NHLBI NIH HHS/United States GR - 23IPA1053651, 26BIPA1622618, 24DIVSUP1287163/American Heart Association/ PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Reactive Oxygen Species) RN - EC 1.6.3.- (NADPH Oxidases) RN - EC 1.14.13.39 (Nitric Oxide Synthase Type III) SB - IM MH - Humans MH - Oxidation-Reduction MH - Animals MH - Reactive Oxygen Species/metabolism MH - *Endothelium, Vascular/metabolism/pathology MH - Signal Transduction MH - *Pulmonary Arterial Hypertension/metabolism/pathology MH - Oxidative Stress MH - NADPH Oxidases/metabolism MH - Mitochondria/metabolism MH - Endothelial Cells/metabolism MH - Nitric Oxide Synthase Type III/metabolism PMC - PMC13705505 OTO - NOTNLM OT - NADPH oxidase OT - circulating biomarkers OT - endothelial dysfunction OT - mechanotransduction OT - mitochondria OT - nitric oxide OT - oxidative stress OT - pulmonary arterial hypertension OT - reactive oxygen species OT - redox signaling COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/14 CRDT- 2026/10/27 10:40 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - ijms27209096 [pii] AID - ijms-27-09096 [pii] AID - 10.3390/ijms27209096 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 14;27(20):9096. doi: 10.3390/ijms27209096. PMID- 42941865 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 14 TI - Circulating Tumour Cell GALNT13 Expression Is Associated with Prognosis and Treatment Response in Non-Small Cell Lung Cancer. LID - 10.3390/ijms27209095 [doi] LID - 9095 AB - Reliable circulating biomarkers for prognosis and treatment monitoring in non-small cell lung cancer (NSCLC) remain limited. Liquid biopsy offers a minimally invasive approach to identify clinically informative tumour-derived markers. Given the emerging role of GalNAc-transferases in cancer biology, we evaluated GALNT13 expression in peripheral blood as a potential biomarker for prognosis and disease monitoring in lung cancer patients. Sensitive molecular assays (nested and digital RT-PCR) were developed to detect GALNT13 mRNA in circulating tumour cells (CTCs). These methods were applied to peripheral blood samples from non-small cell lung cancer patients to assess prognostic value and enable longitudinal monitoring. A cohort of 143 NSCLC patients was analysed, of whom 60% were GALNT13-positive in peripheral blood at baseline. During a five-year follow-up, GALNT13 positivity was significantly associated with reduced overall survival. In several patients, increases in GALNT13 levels preceded radiologic evidence of disease progression. GALNT13 expression in CTCs emerges as a promising biomarker for prognosis and treatment monitoring in NSCLC. Prospective studies in larger cohorts are warranted to validate its clinical utility. FAU - Fernandez Armua, Eugenia AU - Fernandez Armua E AUID- ORCID: 0000-0001-9087-397X AD - Laboratorio de Oncologia Molecular, Servicio de Oncologia, Hospital Maciel, Administracion de Servicios de Salud del Estado (ASSE), 25 de Mayo 174, Montevideo 11000, Uruguay. AD - Laboratorio de Glicobiologia e Inmunologia Tumoral, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo 11400, Uruguay. FAU - Serrano, Maria Jose AU - Serrano MJ AUID- ORCID: 0000-0003-0298-9054 AD - Liquid Biopsy and Cancer Interception Group, Centre for Genomics and Oncological Research (GENyO), Pfizer/University of Granada/Andalusian Regional Government, PTS Granada, Avenida de la Ilustracion 114, 18016 Granada, Spain. AD - Pathological Anatomy Unit (UPIGAP), University Hospital Virgen de las Nieves, Av. de las Fuerzas Armadas 2, 18014 Granada, Spain. AD - Instituto de Investigacion Biosanitaria ibs.GRANADA, Av. de Madrid 15, Beiro, 18012 Granada, Spain. FAU - Santana, Diego AU - Santana D AUID- ORCID: 0009-0009-8408-1269 AD - Laboratorio de Oncologia Molecular, Servicio de Oncologia, Hospital Maciel, Administracion de Servicios de Salud del Estado (ASSE), 25 de Mayo 174, Montevideo 11000, Uruguay. FAU - Carrasco, Mariana AU - Carrasco M AUID- ORCID: 0000-0002-8888-0780 AD - Laboratorio de Oncologia Molecular, Servicio de Oncologia, Hospital Maciel, Administracion de Servicios de Salud del Estado (ASSE), 25 de Mayo 174, Montevideo 11000, Uruguay. FAU - Molina, Maria Pilar AU - Molina MP AD - Liquid Biopsy and Cancer Interception Group, Centre for Genomics and Oncological Research (GENyO), Pfizer/University of Granada/Andalusian Regional Government, PTS Granada, Avenida de la Ilustracion 114, 18016 Granada, Spain. FAU - Aznar, Ines AU - Aznar I AUID- ORCID: 0000-0003-4902-214X AD - Liquid Biopsy and Cancer Interception Group, Centre for Genomics and Oncological Research (GENyO), Pfizer/University of Granada/Andalusian Regional Government, PTS Granada, Avenida de la Ilustracion 114, 18016 Granada, Spain. AD - Instituto de Investigacion Biosanitaria ibs.GRANADA, Av. de Madrid 15, Beiro, 18012 Granada, Spain. FAU - Acosta, Marcos AU - Acosta M AD - Laboratorio de Oncologia Molecular, Servicio de Oncologia, Hospital Maciel, Administracion de Servicios de Salud del Estado (ASSE), 25 de Mayo 174, Montevideo 11000, Uruguay. FAU - Villar, Beatriz AU - Villar B AUID- ORCID: 0009-0009-3328-762X AD - Servicio de Hemoterapia, Hospital Maciel, ASSE, 25 de Mayo 174, Montevideo 11000, Uruguay. FAU - Ferrari, Juan AU - Ferrari J AD - Servicio de Hemoterapia, Hospital Maciel, ASSE, 25 de Mayo 174, Montevideo 11000, Uruguay. FAU - Touya, Diego AU - Touya D AD - Laboratorio de Oncologia Molecular, Servicio de Oncologia, Hospital Maciel, Administracion de Servicios de Salud del Estado (ASSE), 25 de Mayo 174, Montevideo 11000, Uruguay. FAU - Osinaga, Eduardo AU - Osinaga E AD - Laboratorio de Oncologia Molecular, Servicio de Oncologia, Hospital Maciel, Administracion de Servicios de Salud del Estado (ASSE), 25 de Mayo 174, Montevideo 11000, Uruguay. AD - Laboratorio de Glicobiologia e Inmunologia Tumoral, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo 11400, Uruguay. AD - Departamento de Inmunobiologia, Facultad de Medicina, Universidad de la Republica, Av. General Flores 2125, Montevideo 11800, Uruguay. FAU - Berois, Nora AU - Berois N AUID- ORCID: 0000-0002-6372-2540 AD - Laboratorio de Oncologia Molecular, Servicio de Oncologia, Hospital Maciel, Administracion de Servicios de Salud del Estado (ASSE), 25 de Mayo 174, Montevideo 11000, Uruguay. AD - Laboratorio de Glicobiologia e Inmunologia Tumoral, Institut Pasteur de Montevideo, Mataojo 2020, Montevideo 11400, Uruguay. LA - eng GR - 908/University of the Republic/ GR - COF 03/11/Fondo para la Convergencia Estructural del MERCOSUR/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - EC 2.4.1.- (N-Acetylgalactosaminyltransferases) RN - EC 2.4.1.41 (Polypeptide N-acetylgalactosaminyltransferase) RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - *N-Acetylgalactosaminyltransferases/genetics/blood/metabolism MH - *Carcinoma, Non-Small-Cell Lung/blood/pathology/therapy/genetics/mortality MH - *Neoplastic Cells, Circulating/metabolism/pathology MH - Female MH - *Lung Neoplasms/blood/pathology/therapy/genetics MH - Prognosis MH - Polypeptide N-acetylgalactosaminyltransferase MH - *Biomarkers, Tumor/blood/genetics MH - Male MH - Middle Aged MH - Aged MH - Gene Expression Regulation, Neoplastic MH - Treatment Outcome PMC - PMC13705257 OTO - NOTNLM OT - GALNT13 OT - O-glycosylation OT - biomarker OT - cancer OT - circulating tumour cells (CTCs) OT - liquid biopsy OT - non-small cell lung cancer (NSCLC) COIS- D.T. declares the following financial interests/personal relationships which may be considered as potential competing interests: Consulting fees: Scienza, Bristol-Myers Squibb; support for meetings: Roche, Adium. Other authors declare no competing interests. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/14 CRDT- 2026/10/27 10:40 PHST- 2026/09/12 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - ijms27209095 [pii] AID - ijms-27-09095 [pii] AID - 10.3390/ijms27209095 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 14;27(20):9095. doi: 10.3390/ijms27209095. PMID- 42941863 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 14 TI - HDAC Inhibitors Potentiate Doxorubicin Cytotoxicity in HepG2 Cells and Alter DNA-Repair- and p53-Associated Transcriptional Programs. LID - 10.3390/ijms27209091 [doi] LID - 9091 AB - Histone deacetylase inhibitors (HDACis) can enhance responses to DNA-damaging chemotherapy, but how they alter doxorubicin (DOX)-induced transcriptional programs remains incompletely understood. We investigated the effects of valproic acid (VPA) and vorinostat (SAHA) on DOX cytotoxicity and associated molecular responses in HepG2 cells. HDAC inhibition, cell viability, apoptosis, and DOX IC(50) values were assessed using histone H3 acetylation, cell counting, Alamar Blue assays, and cleaved caspase-3 immunofluorescence. Transcriptomic responses were analyzed by RNA sequencing, differential expression analysis, and Hallmark gene set enrichment analysis, with selected findings evaluated by RT-qPCR, Western blotting, and comparison with published CRISPR/Cas9 DOX-response data. Both HDAC inhibitors increased histone H3 acetylation and enhanced DOX cytotoxicity, markedly reducing DOX IC(50) values. VPA co-treatment also increased DOX-induced cleaved caspase-3 signaling. RNA-seq showed broad transcriptional remodeling, including depletion of the DNA-repair gene set following VPA treatment and attenuation of canonical p53-responsive genes during HDACi-DOX co-treatment. Several HDACi-repressed DNA-repair genes overlapped with genes whose loss sensitized cells to DOX in a published CRISPR screen. HDAC inhibition therefore enhances DOX cytotoxicity and is accompanied by alterations in DNA-repair-associated and p53-responsive transcriptional programs. FAU - Guo, Kaidi AU - Guo K AD - Department of Translational Genomics, GROW-Research Institute for Oncology and Reproduction, Maastricht University, 6229 ER Maastricht, The Netherlands. FAU - van Herwijnen, Marcel AU - van Herwijnen M AD - Department of Translational Genomics, GROW-Research Institute for Oncology and Reproduction, Maastricht University, 6229 ER Maastricht, The Netherlands. FAU - Caiment, Florian AU - Caiment F AUID- ORCID: 0000-0002-3325-0466 AD - Department of Translational Genomics, GROW-Research Institute for Oncology and Reproduction, Maastricht University, 6229 ER Maastricht, The Netherlands. FAU - van den Beucken, Twan AU - van den Beucken T AUID- ORCID: 0000-0002-2233-7558 AD - Department of Translational Genomics, GROW-Research Institute for Oncology and Reproduction, Maastricht University, 6229 ER Maastricht, The Netherlands. LA - eng GR - 114027006/ZonMw, The Dutch Organisation for knowledge and innovation in health, healthcare and well-being/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Histone Deacetylase Inhibitors) RN - 80168379AG (Doxorubicin) RN - 0 (Tumor Suppressor Protein p53) RN - 614OI1Z5WI (Valproic Acid) RN - 58IFB293JI (Vorinostat) SB - IM MH - Humans MH - *Histone Deacetylase Inhibitors/pharmacology MH - *Doxorubicin/pharmacology MH - *Tumor Suppressor Protein p53/metabolism/genetics MH - Hep G2 Cells MH - Valproic Acid/pharmacology MH - *DNA Repair/drug effects MH - Cell Survival/drug effects MH - Apoptosis/drug effects MH - Vorinostat/pharmacology MH - *Transcription, Genetic/drug effects MH - Drug Synergism MH - Gene Expression Regulation, Neoplastic/drug effects MH - Acetylation/drug effects PMC - PMC13705291 OTO - NOTNLM OT - Chemosensitization/drug sensitization OT - DNA repair and p53 signaling OT - HepG2 cells OT - RNA-seq transcriptomics OT - doxorubicin OT - histone deacetylase inhibitors (HDACis) COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/14 CRDT- 2026/10/27 10:40 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - ijms27209091 [pii] AID - ijms-27-09091 [pii] AID - 10.3390/ijms27209091 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 14;27(20):9091. doi: 10.3390/ijms27209091. PMID- 42941861 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 14 TI - Molecular Targeting of the Peripheral Nerve-Tumor Axis in Extracranial Solid Cancers: Eight Signaling Systems, One Effect-Systematic Review and Multilevel Meta-Analysis. LID - 10.3390/ijms27209090 [doi] LID - 9090 AB - The peripheral nerves that innervate solid tumors communicate with the malignant cells through a restricted set of molecular signaling systems: adrenergic and muscarinic receptors, sensory neuropeptides, neurotrophins and their tyrosine kinase receptors, axon guidance molecules, and nociceptor ion channels. Each has been proposed separately as a therapeutic target but never quantitatively compared with the others. The question is whether one of them dominates-or whether the real target is the axis as a system. We systematically searched PubMed, Scopus, and Web of Science, with four query modules per database, without year or language restriction. We included in vivo studies in mammals, on extracranial solid cancers, with an intervention that modulated tumor innervation, peripheral nerve activity, or neural signaling, and with at least one tumor burden outcome. Of 16,649 unique records, 362 were included, with 1291 experiments and 1437 effects. Blockade of the peripheral nerve-tumor axis reduced tumor burden by -44.8%. In the prespecified primary analysis, on established tumors, the reduction was -52.0% with no significant difference from preventive interventions (p = 0.17). All eight molecular signaling systems responded to blockade, with estimates ranging from -34.3% for TRP channels to -70.2% for glutamatergic signaling. Interventions that activate the axis produced the opposite effect, with a 52.1% increase (95% CI: 32.1 to 75.1) across 253 effects, and bidirectionality was demonstrated within the same molecular system for the adrenergic compartment (-42.8% for blockade vs. 58.1% for activation), axon guidance molecules, and TRP channels. Egger's test indicated marked small-study effects (slope -1.12; p < 0.001); the estimate remained stable when low-confidence digitized values were excluded (-42.6%). Eight distinct molecular signaling systems, disrupted by methods with nothing in common, produce reductions of a similar magnitude in tumor burden, and stimulation of three of them produces the opposite effect. FAU - Tudorascu, Diana-Rodica AU - Tudorascu DR AD - Department of Medical Semiology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. FAU - Vere, Cristin Constantin AU - Vere CC AD - Department of Gastroenterology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Petrescu, Mihai AU - Petrescu M AD - Department of Psychiatry, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Ciurea, Ana-Maria AU - Ciurea AM AD - Department of Oncology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Pana, Razvan-Cosmin AU - Pana RC AD - Department of Gynecology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. FAU - Dan, Alexandra Oltea AU - Dan AO AD - Experimental Research Centre for Normal and Pathological Aging, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. FAU - Tartea, Elena-Anca AU - Tartea EA AD - Department of Neurology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. FAU - Gresita, Andrei AU - Gresita A AD - Experimental Research Centre for Normal and Pathological Aging, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. FAU - Hadareanu, Diana-Ruxandra AU - Hadareanu DR AUID- ORCID: 0000-0002-0500-0312 AD - Department of Cardiology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. FAU - Obleaga, Cosmin Vasile AU - Obleaga CV AD - Department of Surgical Oncology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Tartea, Georgica AU - Tartea G AD - Experimental Research Centre for Normal and Pathological Aging, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. AD - Department of Cardiology, University of Medicine and Pharmacy of Craiova, 2 Petru Rares Street, 200349 Craiova, Romania. LA - eng PT - Journal Article PT - Meta-Analysis PT - Review PT - Systematic Review DEP - 20261014 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - Humans MH - *Signal Transduction/drug effects MH - *Neoplasms/metabolism/drug therapy/pathology MH - Animals MH - *Peripheral Nerves/metabolism/drug effects/pathology MH - *Molecular Targeted Therapy PMC - PMC13705328 OTO - NOTNLM OT - adrenergic receptors OT - axon guidance molecules OT - cancer neuroscience OT - muscarinic receptors OT - neurotrophins OT - perineural invasion OT - tumor innervation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/14 CRDT- 2026/10/27 10:40 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - ijms27209090 [pii] AID - ijms-27-09090 [pii] AID - 10.3390/ijms27209090 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 14;27(20):9090. doi: 10.3390/ijms27209090. PMID- 42941856 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 13 TI - The Role of SOD1 and Oxidative Stress in Amyotrophic Lateral Sclerosis: An Overview. LID - 10.3390/ijms27209084 [doi] LID - 9084 AB - Amyotrophic lateral sclerosis (ALS) is a rapidly progressing neurodegenerative disorder that selectively affects motor neurons. The median survival time after diagnosis is 2 to 5 years. Oxidative stress (OS), driven mainly by reactive oxygen species (ROS), plays a central role in motor neuron (MN) degeneration. In this narrative review, we analyse the pathogenic role of SOD1 variants and their contribution to OS in ALS. SOD1 mutations alter the Cu/Zn superoxide dismutase enzyme, thereby promoting oxidative damage and triggering neuroinflammatory cascades via the MAPK, NF-kappaB and Nrf2/ARE signalling pathways. We also discuss emerging proteomic and machine-learning biomarkers, as well as the current therapeutic landscape. Riluzole and edaravone are approved treatments, while AMX0035 was approved in 2022 but withdrawn in 2024. Masitinib and EPI-589 remain investigational. In the realm of genetic therapies, the antisense oligonucleotide tofersen received accelerated FDA approval in 2023 for SOD1-ALS, primarily due to its ability to reduce plasma neurofilament light chain levels. However, the clinical benefit remains modest. RNA interference has reached human trials, while CRISPR/Cas9 remains in the preclinical stage. Both approaches have shown promising results in experimental models. Further research into ALS pathogenesis is needed to develop treatments that integrate antioxidant, anti-inflammatory, and gene-editing strategies. FAU - Gomez-Perez, Carlos Alberto AU - Gomez-Perez CA AD - Departamento de Genetica, Instituto Nacional de Neurologia y Neurocirugia, Mexico City 14269, Mexico. AD - Posgrado en Ciencias Genomicas, Universidad Autonoma de la Ciudad de Mexico, Mexico City 03104, Mexico. FAU - Ramirez-Garcia, Miguel Angel AU - Ramirez-Garcia MA AUID- ORCID: 0000-0003-3221-3636 AD - Departamento de Genetica, Instituto Nacional de Neurologia y Neurocirugia, Mexico City 14269, Mexico. FAU - Yescas-Gomez, Petra AU - Yescas-Gomez P AD - Departamento de Genetica, Instituto Nacional de Neurologia y Neurocirugia, Mexico City 14269, Mexico. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - EC 1.15.1.1 (Superoxide Dismutase-1) RN - 0 (SOD1 protein, human) RN - 0 (Reactive Oxygen Species) SB - IM MH - Humans MH - *Amyotrophic Lateral Sclerosis/metabolism/genetics/therapy/pathology MH - *Superoxide Dismutase-1/genetics/metabolism MH - *Oxidative Stress MH - Animals MH - Reactive Oxygen Species/metabolism MH - Mutation MH - Motor Neurons/metabolism/pathology MH - Signal Transduction PMC - PMC13705199 OTO - NOTNLM OT - ROS OT - SOD1 OT - gene therapy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/13 CRDT- 2026/10/27 10:40 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - ijms27209084 [pii] AID - ijms-27-09084 [pii] AID - 10.3390/ijms27209084 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 13;27(20):9084. doi: 10.3390/ijms27209084. PMID- 42941854 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 13 TI - Profiling Systemic IgG-Targeted Gut Microbiota Reveals Disease Activity-Associated Bacterial Signatures in Ulcerative Colitis. LID - 10.3390/ijms27209081 [doi] LID - 9081 AB - Aberrant systemic IgG responses to the gut microbiota may be implicated in the pathogenesis of ulcerative colitis (UC). In this study, we investigated IgG-targeted bacteria and their predicted functional characteristics in UC. Using fecal samples and autologous sera from 37 patients with UC and 4 healthy controls, we profiled IgG-targeted bacteria under in vitro conditions using 16S rRNA gene sequencing. To correct for background bacterial abundance, we calculated the IgG+ Probability Score (IPS), which integrates the sample-specific IgG-binding rate to isolate candidate IgG-targeted taxa. Systemic IgG responses were selectively targeted at specific bacterial taxa rather than uniformly directed toward the entire gut microbiota. Active UC and elevated systemic inflammatory markers were associated with increased IPS in oral-associated commensals, including Granulicatella, as well as mucin-degrading taxa. Predictive functional profiling indicated the predicted enrichment of pathways hypothesized to be related to host-derived mucin degradation, capsular polysaccharide biosynthesis, and amino acid biosynthesis. Furthermore, exploratory Random Forest models using IPS features highlighted candidate taxa that differed among clinical parameters (UC exacerbation, Clinical Activity Index, and C-reactive protein), although their classification performance was not independently validated. In conclusion, our in vitro profiling indicates that systemic IgG in UC has the capacity to selectively target specific bacterial taxa associated with disease activity and systemic inflammation. Analyzing the IgG-targeted microbiota via IPS profiling deepens our understanding of host-microbiota interactions in UC and provides a valuable framework for evaluating candidate immune-targeted bacteria associated with disease activity. FAU - Takahashi, Koichi AU - Takahashi K AD - Department of Microbiology, Faculty of Medicine, Kagawa University, 1750-1 Miki-cho, Kita-gun, Kagawa 761-0793, Japan. AD - Department of Pharmacy, Kagawa University Hospital, 1750-1 Miki, Kagawa 761-0793, Japan. FAU - Nakayama-Imaohji, Haruyuki AU - Nakayama-Imaohji H AD - Department of Microbiology, Faculty of Medicine, Kagawa University, 1750-1 Miki-cho, Kita-gun, Kagawa 761-0793, Japan. FAU - Tada, Ayano AU - Tada A AD - Department of Microbiology, Faculty of Medicine, Kagawa University, 1750-1 Miki-cho, Kita-gun, Kagawa 761-0793, Japan. FAU - Emmanuel, Munyeshyaka AU - Emmanuel M AD - Department of Microbiology, Faculty of Medicine, Kagawa University, 1750-1 Miki-cho, Kita-gun, Kagawa 761-0793, Japan. FAU - Mino, Yasuaki AU - Mino Y AUID- ORCID: 0000-0002-5706-1117 AD - Department of Pharmacy, Kagawa University Hospital, 1750-1 Miki, Kagawa 761-0793, Japan. FAU - Ishikawa, Hideki AU - Ishikawa H AD - Department of Molecular-Targeting Prevention, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 3-2-17-2F Imabashi, Chuo-ku, Osaka 541-0042, Japan. FAU - Kuwahara, Tomomi AU - Kuwahara T AD - Department of Microbiology, Faculty of Medicine, Kagawa University, 1750-1 Miki-cho, Kita-gun, Kagawa 761-0793, Japan. LA - eng GR - 17K08833/Japan Society for the Promotion of Science/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Immunoglobulin G) RN - 0 (RNA, Ribosomal, 16S) SB - IM MH - Humans MH - *Colitis, Ulcerative/microbiology/immunology MH - *Immunoglobulin G/immunology MH - Female MH - RNA, Ribosomal, 16S/genetics MH - *Gastrointestinal Microbiome/immunology MH - Male MH - Feces/microbiology MH - *Bacteria/genetics/immunology/classification MH - Adult MH - Middle Aged PMC - PMC13705514 OTO - NOTNLM OT - 16S rRNA gene analysis OT - IgG+ probability score OT - gut microbiota OT - ulcerative colitis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/13 CRDT- 2026/10/27 10:40 PHST- 2026/08/07 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - ijms27209081 [pii] AID - ijms-27-09081 [pii] AID - 10.3390/ijms27209081 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 13;27(20):9081. doi: 10.3390/ijms27209081. PMID- 42941853 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 13 TI - Normalization Strategies for miRNA RT-qPCR in Blood-Derived Samples in Obesity Research: Current Practices and Methodological Considerations. LID - 10.3390/ijms27209079 [doi] LID - 9079 AB - Reverse transcription quantitative PCR (RT-qPCR) is widely used to quantify circulating microRNAs, but reliable interpretation depends on appropriate data normalization. In obesity research, selecting reference RNAs for measurements in whole blood, blood cells, plasma, and serum is challenging because of biological heterogeneity and preanalytical variability. This review summarizes normalization strategies used in RT-qPCR studies of blood-derived samples from participants with overweight or obesity. We examine commonly used endogenous reference RNAs, including U6 snRNA and miR-16-5p, and assess the rationale for their selection and the available validation evidence in relation to sample matrix and cohort characteristics. The review evaluates normalization practices and the quality and reporting of reference-RNA validation in obesity-related studies, with particular attention to the biological comparison being made. No candidate, algorithmic ranking, or fixed panel size is considered universally applicable. Candidate selection, the number of references, and the normalization scale should be justified for the intended comparison. The review provides practical guidance for designing and reporting validation of reference small non-coding RNAs and process controls in study cohorts with overweight or obesity, with recommendations qualified by the available evidence. FAU - Pustovalova, Anna V AU - Pustovalova AV AUID- ORCID: 0009-0009-8741-6650 AD - Research Centre for Medical Genetics, 115522 Moscow, Russia. FAU - Nikolaeva, Alexandra F AU - Nikolaeva AF AD - Research Centre for Medical Genetics, 115522 Moscow, Russia. FAU - Kalinkin, Alexey I AU - Kalinkin AI AUID- ORCID: 0000-0001-9215-4581 AD - Research Centre for Medical Genetics, 115522 Moscow, Russia. FAU - Sigin, Vladimir O AU - Sigin VO AD - Research Centre for Medical Genetics, 115522 Moscow, Russia. LA - eng GR - 25-25-00826/Russian Science Foundation/ PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (MicroRNAs) RN - 0 (RNA, Small Nuclear) RN - 0 (U6 small nuclear RNA) SB - IM MH - Humans MH - *Obesity/genetics/blood MH - *MicroRNAs/blood/genetics MH - Reference Standards MH - RNA, Small Nuclear/genetics/blood MH - *Real-Time Polymerase Chain Reaction/methods/standards PMC - PMC13705478 OTO - NOTNLM OT - microRNA OT - obesity OT - reference genes COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/13 CRDT- 2026/10/27 10:40 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - ijms27209079 [pii] AID - ijms-27-09079 [pii] AID - 10.3390/ijms27209079 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 13;27(20):9079. doi: 10.3390/ijms27209079. PMID- 42941851 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 13 TI - Immunomodulatory Effects of Fractional CO(2) Laser and Narrowband UVB in Stable Non-Segmental Vitiligo. LID - 10.3390/ijms27209076 [doi] LID - 9076 AB - Stable non-segmental vitiligo is sustained by persistent autoimmune responses involving tissue-resident memory T (TRM) cells, impaired regulatory T-cell (Treg) function, and chronic cytokine-mediated inflammation. Although fractional carbon dioxide (CO(2)) laser and narrowband ultraviolet B (NB-UVB) phototherapy are widely used to induce repigmentation, their immunomodulatory mechanisms remain incompletely understood. In this prospective, non-randomized exploratory study, 19 patients with stable non-segmental vitiligo received either monthly fractional CO(2) laser monotherapy or fractional CO(2) laser combined with NB-UVB phototherapy for 6 months. Serum IL-15 and IL-17A concentrations were quantified by ELISA at baseline, 3 months, and 6 months. Double immunofluorescence was performed to evaluate cutaneous TRM- and Treg-associated markers (CD4, CD8, CD25, CD49a, CD69, CD103, FOXP3) together with melanocyte-associated markers (MITF and c-KIT). Longitudinal differences between treatment groups were assessed using linear mixed-effects models with treatment-by-time interactions. Serum IL-15 and IL-17A concentrations decreased significantly over time in both treatment groups. At 6 months, IL-15 concentrations did not differ significantly from those in healthy controls in either group, whereas IL-17A concentrations did not differ significantly from healthy-control values in the fractional CO(2) laser monotherapy group but remained significantly higher in the combination-treatment group. Cutaneous analyses demonstrated longitudinal changes in selected TRM- and Treg-associated markers and melanocyte-associated markers, with MITF showing the most pronounced increase. Several cutaneous immune markers remained significantly different from healthy-control values after 6 months. No statistically significant treatment-by-time interactions were detected for IL-15, IL-17A, or the evaluated dermal and epidermal markers. Fractional CO(2) laser monotherapy and fractional CO(2) laser combined with NB-UVB were both associated with longitudinal changes in circulating inflammatory cytokines and selected cutaneous immune- and melanocyte-associated markers in patients with stable non-segmental vitiligo. Persistent abnormalities in several cutaneous immune markers suggest that treatment did not fully restore the cutaneous immune-marker profile within the 6-month observation period. Given the small sample size and non-randomized exploratory design, the absence of statistically significant treatment-by-time interactions should not be interpreted as evidence of therapeutic equivalence, non-inferiority, or absence of an additional effect of NB-UVB. FAU - Fraczek, Alicja AU - Fraczek A AD - Clinic and Department of Dermatology, Sexually Transmitted Diseases and Clinical Immunology, School of Medicine, University Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland. FAU - Czerwinska, Joanna AU - Czerwinska J AUID- ORCID: 0000-0001-6371-8130 AD - Clinic and Department of Dermatology, Sexually Transmitted Diseases and Clinical Immunology, School of Medicine, University Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland. FAU - Zaluski, Dariusz AU - Zaluski D AUID- ORCID: 0000-0002-5904-3035 AD - Department of Plant Breeding and Bioresource Engineering, University of Warmia and Mazury, Plac Lodzki 3/422, 10-727 Olsztyn, Poland. FAU - Owczarczyk-Saczonek, Agnieszka AU - Owczarczyk-Saczonek A AUID- ORCID: 0000-0002-8372-0438 AD - Clinic and Department of Dermatology, Sexually Transmitted Diseases and Clinical Immunology, School of Medicine, University Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Interleukin-17) RN - 0 (Interleukin-15) RN - 0 (Biomarkers) SB - IM MH - Humans MH - *Vitiligo/immunology/therapy/radiotherapy/blood MH - Female MH - *Lasers, Gas/therapeutic use MH - Male MH - Adult MH - T-Lymphocytes, Regulatory/immunology MH - Middle Aged MH - *Ultraviolet Therapy/methods MH - Interleukin-17/blood MH - Interleukin-15/blood MH - Prospective Studies MH - *Immunomodulation/radiation effects MH - Biomarkers MH - Melanocytes/metabolism PMC - PMC13705425 OTO - NOTNLM OT - IL-15 OT - IL-17A OT - fractional carbon dioxide laser OT - immunofluorescence OT - immunomodulation OT - melanocyte regeneration OT - narrowband ultraviolet B OT - regulatory T cells OT - tissue-resident memory T cells OT - vitiligo COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/13 CRDT- 2026/10/27 10:40 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - ijms27209076 [pii] AID - ijms-27-09076 [pii] AID - 10.3390/ijms27209076 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 13;27(20):9076. doi: 10.3390/ijms27209076. PMID- 42941850 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 13 TI - To Be or Not to Be-Thymocyte Survival and Death in the Thymic Medulla. LID - 10.3390/ijms27209078 [doi] LID - 9078 AB - The T cells of the mammalian immune system have a tricky challenge. They must recognize foreign antigen as "other" or "non-self" but in the context of self, while also being tolerant to the body's own molecules and tissues. T-cell receptor (TCR) gene rearrangement occurs in the cortex of the thymus and generates receptors of relatively random specificity. The developing T cells (thymocytes) are screened in two stages, both of which are Major Histocompatibility Complex (MHC)-based. The first, positive selection, occurs in the thymic cortex where cortical thymic epithelial cells (cTECs) display self MHC + self-peptides. Thymocytes that can bind to self MHC + self-peptide then migrate to the corticomedullary junction, where they undergo negative selection. Here, they encounter antigenic peptides characteristic of peripheral tissue proteins presented by medullary antigen-presenting cells (APCs). Among the most intriguing APCs in the medulla are the mimetic medullary thymic epithelial cells (mimetic mTECs). These mimetic APCs express peripheral antigens under the direction of specific transcription factors. During negative selection, most thymocytes whose TCRs bind too well to self MHC + self-peptide die by apoptosis. However, a small proportion of autoreactive thymocytes escape negative selection and instead "alternatively" differentiate into specialized cell types that are self-tolerant and even enforce self-tolerance. The CD4 and CD8 coreceptors, which bind to MHC II and MHC I, respectively, play crucial roles in positive and negative selection. With CD8, there is an added level of complexity because there are two forms of CD8, only one of which can promote signaling through the TCR. Upon binding to MHC, CD4 and CD8 initiate signal transduction through the TCR. The strength of signal transduction through the TCR and through co-stimulatory CD28 determines the outcome of TCR-MHC + peptide binding. Signal transduction occurs within the immunological synapse and is regulated by kinetic proofreading mechanisms. In summary, negative selection involves interactions between developing thymocytes and a variety of medullary APCs. These molecular interactions occur at specialized membrane domains that bring together the components of the TCR signaling apparatus, which are subject to kinetic proofreading. Together, the regulatory mechanisms occurring in developing thymocytes and in medullary APCs generate a T-cell arsenal that is tolerant of self and active against foreign entities. FAU - Zuniga, Martha C AU - Zuniga MC AD - Department of Molecular, Cell, and Developmental Biology, University of California, Santa Cruz, CA 95064, USA. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Receptors, Antigen, T-Cell) SB - IM MH - Animals MH - *Thymocytes/immunology/cytology/metabolism MH - *Thymus Gland/immunology/cytology MH - Humans MH - Cell Survival MH - Receptors, Antigen, T-Cell/metabolism MH - Apoptosis MH - Epithelial Cells/immunology MH - Major Histocompatibility Complex PMC - PMC13705424 OTO - NOTNLM OT - CD8alphabeta OT - PD-1 OT - immunological synapse OT - kinetic proofreading OT - mimetic medullary thymic epithelial cells OT - negative selection OT - self-tolerance OT - thymocyte development COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/13 CRDT- 2026/10/27 10:40 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - ijms27209078 [pii] AID - ijms-27-09078 [pii] AID - 10.3390/ijms27209078 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 13;27(20):9078. doi: 10.3390/ijms27209078. PMID- 42941849 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 13 TI - Store-Operated Calcium Entry Inhibitors: Structural Diversity, Mechanisms of Action, and Optimization Strategies. LID - 10.3390/ijms27209077 [doi] LID - 9077 AB - Store-operated calcium entry (SOCE) is a ubiquitous calcium influx pathway critically involved in numerous physiological processes. Dysregulated SOCE contributes to the pathogenesis of cardiovascular disorders, cancer, and neurodegenerative diseases, making SOCE modulators promising therapeutic candidates. This review provides an overview of the major chemical classes of classical SOCE inhibitors that target Orai and STIM proteins. We focus on structure-activity relationships (SARs), mechanisms of action, strategies for enhancing inhibitory potency and selectivity, and discuss the limitations and side-effect profiles of these compounds. This comprehensive analysis identifies SOC channels as promising therapeutic targets and outlines future directions for rational drug design and the development of clinically viable SOCE modulators. FAU - Zakharova, Anastasiia A AU - Zakharova AA AD - Institute of Cytology of Russian Academy of Science, Tikhoretsky Ave. 4, 194064 St. Petersburg, Russia. FAU - Grekhnev, Dmitriy A AU - Grekhnev DA AUID- ORCID: 0000-0003-2338-6252 AD - Institute of Cytology of Russian Academy of Science, Tikhoretsky Ave. 4, 194064 St. Petersburg, Russia. FAU - Kaznacheyeva, Elena V AU - Kaznacheyeva EV AUID- ORCID: 0000-0003-1273-1188 AD - Institute of Cytology of Russian Academy of Science, Tikhoretsky Ave. 4, 194064 St. Petersburg, Russia. AD - Center of Genetic Reprogramming and Gene Therapy, Institute of Cytology of Russian Academy of Science, Tikhoretsky Ave. 4, 194064 St. Petersburg, Russia. FAU - Vigont, Vladimir A AU - Vigont VA AUID- ORCID: 0000-0001-8389-7316 AD - Institute of Cytology of Russian Academy of Science, Tikhoretsky Ave. 4, 194064 St. Petersburg, Russia. LA - eng GR - 25-15-00165/Russian Science Foundation/ GR - 075-15-2025-529/the Ministry of Science and Higher Education of the Russian Federation/ PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - SY7Q814VUP (Calcium) RN - 0 (Calcium Channel Blockers) RN - 0 (Calcium Channels) SB - IM MH - Humans MH - *Calcium/metabolism MH - Animals MH - *Calcium Channel Blockers/pharmacology/chemistry MH - Structure-Activity Relationship MH - *Calcium Channels/metabolism/chemistry MH - *Calcium Signaling/drug effects PMC - PMC13705031 OTO - NOTNLM OT - Orai OT - SOC channel inhibitors OT - SOCE OT - STIM OT - calcium OT - store-operated calcium entry OT - structure-activity relationship COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/13 CRDT- 2026/10/27 10:40 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - ijms27209077 [pii] AID - ijms-27-09077 [pii] AID - 10.3390/ijms27209077 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 13;27(20):9077. doi: 10.3390/ijms27209077. PMID- 42941847 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 13 TI - Construction and Characterization of Rabbit scFv Antibodies Targeting Fibronectin by Phage Display Technology. LID - 10.3390/ijms27209074 [doi] LID - 9074 AB - Fibronectin (FN) is a multifunctional glycoprotein involved in extracellular matrix remodeling, tumor progression, tissue repair, and host-pathogen interactions. Despite its biological and clinical relevance, FN-binding antibodies derived from non-rodent species remain comparatively less explored. Here, one rabbit was immunized with full-length recombinant human FN, eliciting a robust antibody response confirmed by ELISA and Western blotting. Two rabbit-derived single-chain variable fragment (scFv) phage-display libraries were constructed from peripheral blood mononuclear cells, yielding 5.5 x 10(7) and 5.0 x 10(7) transformants for the short- and long-linker formats, respectively. After four rounds of biopanning, phage ELISA confirmed enrichment, and sequence analysis grouped selected clones into five short-linker and four long-linker groups. Seven purified scFvs displayed distinct ELISA binding profiles. Fiber-optic particle plasmon resonance (FOPPR)-based kinetic analysis yielded apparent equilibrium dissociation constants (K(D,app)) of 11.21-432.06 nM, with FN-L13 showing the lowest K(D,app). Analytical size-exclusion chromatography indicated predominantly monomeric scFvs. Five scFvs recognized a high-molecular-weight band consistent with fibronectin in mouse plasma by Western blotting. ELISA showed preferential FN binding, with generally low reactivity toward human serum albumin (HSA) and collagens I, III, and IV, although FN-S1 and FN-L1 showed detectable fibrinogen reactivity. These findings support rabbit-derived FN-binding scFvs as research reagents for future FN-related assay development and mechanistic studies. FAU - Chiang, Yu-Wei AU - Chiang YW AD - Graduate Institute of Biodefense, National Defense Medical University, Taipei 114201, Taiwan. AD - Institute of Preventive Medicine, National Defense Medical University, Taipei 114201, Taiwan. AD - Department of Occupational Medicine and Clinical Toxicology, Taipei Veterans General Hospital, Taipei 112201, Taiwan. AD - Department of Biology and Anatomy, National Defense Medical University, Taipei 114201, Taiwan. FAU - Juang, Jia-Lin AU - Juang JL AD - Zinexts Life Science Corp., New Taipei City 221416, Taiwan. AD - JW Diagnostics Inc., New Taipei City 235048, Taiwan. FAU - Yang, Yi-Yuan AU - Yang YY AD - Department of New Drug Development, GlycoNex Inc., New Taipei City 221021, Taiwan. FAU - Leu, Sy-Jye AU - Leu SJ AD - Department of R&D, Zinexts Life Science Corp., New Taipei City 221416, Taiwan. FAU - Liu, Jun-Jen AU - Liu JJ AD - School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, New Taipei City 235603, Taiwan. AD - Ph.D. Program in Medical Biotechnology, College of Medical Science and Technology, Taipei Medical University, New Taipei City 235603, Taiwan. FAU - Tsai, Bor-Yu AU - Tsai BY AD - Navi Bio-Therapeutics Inc., Taipei 10351, Taiwan. FAU - Mao, Yan-Chiao AU - Mao YC AUID- ORCID: 0000-0002-8561-0582 AD - Division of Clinical Toxicology, Department of Emergency Medicine, Taichung Veterans General Hospital, Taichung 407219, Taiwan. FAU - Yen, Fang-Yi AU - Yen FY AD - Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming Chiao Tung University, Taipei 112304, Taiwan. FAU - Chiang, Jia-Yun AU - Chiang JY AD - School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, New Taipei City 235603, Taiwan. FAU - Andriani, Valencia AU - Andriani V AD - iReal Biotechnology Inc., Research and Development Center, New Taipei City 248022, Taiwan. FAU - Thenaka, Priskila Cherisca AU - Thenaka PC AUID- ORCID: 0009-0006-6462-8544 AD - Department of Project Management, GlycoNex Inc., New Taipei City 221021, Taiwan. FAU - Tan, Ta-Lun AU - Tan TL AD - Ph.D. Program in Medical Biotechnology, College of Medical Science and Technology, Taipei Medical University, New Taipei City 235603, Taiwan. FAU - Shen, Ting-Jing AU - Shen TJ AUID- ORCID: 0000-0002-1714-0048 AD - School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, New Taipei City 235603, Taiwan. AD - Ph.D. Program in Medical Biotechnology, College of Medical Science and Technology, Taipei Medical University, New Taipei City 235603, Taiwan. FAU - Lee, Chi-Hsin AU - Lee CH AUID- ORCID: 0000-0002-3412-7400 AD - Graduate Institute of Biodefense, National Defense Medical University, Taipei 114201, Taiwan. AD - iReal Biotechnology Inc., Research and Development Center, New Taipei City 248022, Taiwan. LA - eng GR - MOST 109-2320-B-038-053/National Science and Technology Council/ GR - MOST 110-2320-B-075A-001/National Science and Technology Council/ GR - NSTC 114-2320-B-075A-003-MY3-1/National Science and Technology Council/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Fibronectins) RN - 0 (Single-Chain Antibodies) RN - 0 (Peptide Library) RN - 0 (Recombinant Proteins) SB - IM MH - Animals MH - *Fibronectins/immunology MH - Rabbits MH - *Single-Chain Antibodies/immunology/genetics/chemistry MH - Humans MH - *Cell Surface Display Techniques MH - Peptide Library MH - Enzyme-Linked Immunosorbent Assay MH - Protein Binding MH - Antibody Specificity MH - Recombinant Proteins/immunology PMC - PMC13705019 OTO - NOTNLM OT - fibronectin (FN) OT - phage display OT - rabbit antibody OT - single-chain variable fragment (scFv) COIS- Jia-Lin Juang is an employee of Zinexts Life Science Corp. and is also affiliated with JW Diagnostics Inc.; Yi-Yuan Yang and Priskila Cherisca Thenaka are employees of GlycoNex Inc.; Sy-Jye Leu is an employee of Zinexts Life Science Corp.; Bor-Yu Tsai is an employee of Navi Bio-Therapeutics Inc.; and Chi-Hsin Lee and Valencia Andriani are employees of iReal Biotechnology Inc. iReal Biotechnology Inc. and Taipei Medical University provided research resources and institutional support for this study. The remaining authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/13 CRDT- 2026/10/27 10:40 PHST- 2026/08/13 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - ijms27209074 [pii] AID - ijms-27-09074 [pii] AID - 10.3390/ijms27209074 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 13;27(20):9074. doi: 10.3390/ijms27209074. PMID- 42941829 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 13 TI - Recent Developments in Chemo-Photodynamic Nanoplatforms for Glioblastoma Treatment. LID - 10.3390/ijms27209058 [doi] LID - 9058 AB - Glioblastoma multiforme (GBM) remains one of the most difficult malignant brain tumours to treat due to its infiltrative nature, cellular heterogeneity, resistance to treatment and limited drug penetration across the blood-brain barrier. The combination of photodynamic therapy (PDT) with chemotherapy within a single nanoplatform offers the possibility of integrating the photogeneration of reactive oxygen species with the cytotoxic action of chemotherapeutic agents, whilst improving their coordinated delivery to tumour cells. This review summarises the latest nanoparticle-based strategies combining PDT and chemotherapy in the treatment of GBM. The literature published between January 2025 and August 2026 was identified using the PubMed, Web of Science and Scopus databases. The systems analysed included photoreactive organic nanoaggregates, ZnO-based nanocomposites, lipid nanocarriers, dual-targeting liposomes and platforms based on covalent organic frameworks. These nanoplatforms enabled various forms of therapeutic integration, including light-triggered drug release, co-encapsulation of photosensitisers and chemotherapeutic agents, targeting of mitochondria, and targeting of glioma cells and blood-brain barrier models via ligands. The available studies have generally demonstrated increased cytotoxicity following photoactivation, and selected systems have shown synergistic effects between chemotherapy and PDT. However, the available evidence is insufficient to support the translational readiness of the currently reported chemo-PDT nanoplatforms. Three of the five systems were evaluated only in vitro, only one was investigated in an orthotopic glioma model, and several studies relied on surrogate metabolic assays or irradiation conditions of limited relevance to deep intracranial disease. The current literature should therefore be regarded primarily as proof-of-concept evidence for coordinated chemo-PDT delivery rather than as evidence of clinically applicable therapy. Progress towards clinical relevance will require platforms designed around realistic light-delivery constraints, the hypoxic GBM microenvironment, brain-relevant delivery routes and therapeutically meaningful in vivo endpoints. FAU - Woznicki, Pawel AU - Woznicki P AUID- ORCID: 0009-0001-6962-2655 AD - Doctoral School, Faculty of Medicine, Collegium Medicum, University of Rzeszow, 35-310 Rzeszow, Poland. FAU - Aebisher, David AU - Aebisher D AUID- ORCID: 0000-0002-2661-6570 AD - Department of Photomedicine and Physical Chemistry, Faculty of Medicine, Collegium Medicum, University of Rzeszow, 35-310 Rzeszow, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Photosensitizing Agents) RN - 0 (Antineoplastic Agents) RN - 0 (Reactive Oxygen Species) RN - 0 (Liposomes) SB - IM MH - Humans MH - *Glioblastoma/drug therapy MH - *Photochemotherapy/methods MH - *Brain Neoplasms/drug therapy MH - *Photosensitizing Agents/therapeutic use MH - *Antineoplastic Agents/therapeutic use MH - Animals MH - *Nanoparticles/chemistry MH - Blood-Brain Barrier/metabolism MH - Reactive Oxygen Species/metabolism MH - Drug Delivery Systems MH - Liposomes PMC - PMC13705486 OTO - NOTNLM OT - PDT OT - chemotherapy OT - nanoplatform COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/13 CRDT- 2026/10/27 10:40 PHST- 2026/09/22 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - ijms27209058 [pii] AID - ijms-27-09058 [pii] AID - 10.3390/ijms27209058 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 13;27(20):9058. doi: 10.3390/ijms27209058. PMID- 42941815 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 12 TI - Sphingolipid Dysregulation in Type 2 Diabetes and Its Microvascular Complications. LID - 10.3390/ijms27209043 [doi] LID - 9043 AB - Sphingolipids arestructural components of cell membranes and bioactive mediators governing cellular metabolism and intercellular communication. Beyond their classical roles in insulin sensitivity and glucose/lipid homeostasis, ceramide, sphingosine-1-phosphate (S1P), sphingomyelin and glycosphingolipids coordinate through lipid rafts, extracellular vesicles, organelle contact sites and circulating carriers to facilitate signaling among pancreas, liver, adipose tissue, vascular endothelium and kidney. This review summarizes literature on how dietary and endogenous sphingolipids contribute to T2DM and its microvascular complications. Preclinical studies, mainly in cultured cells and rodent models, indicate that overnutrition with excess take of saturated and industrial trans fats promotes ceramide accumulation, which impairs AKT/AMPK signaling, enhancing hepatic gluconeogenesis and beta-cell dysfunction, whereas S1P exerts protective metabolic and vascular effects. Whether these mechanisms are similarly dominant in humans remains to be established. Chain-length-specific ceramides generated by distinct ceramide synthases (CerS1-6) determine organelle and intercellular responses in experimental models. In human observational studies, ceramide/S1P ratios and selected sphingolipid species have been associated with metabolic risk, but their diagnostic and risk-stratification value requires clinical validation. We further discuss nutritional patterns, antidiabetic and lipid-lowering agents, and emerging nanomedicine or exosome-based strategies that restore sphingolipid balance. Targeting compartment- and chain-length-specific sphingolipid signaling may provide more precise therapeutic opportunities for T2DM and its complications. FAU - Wen, Ya AU - Wen Y AUID- ORCID: 0000-0002-1942-2110 AD - Department of Reproductive and Genetics, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China. FAU - Lin, Yayun AU - Lin Y AD - Department of Reproductive and Genetics, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China. FAU - Liu, Yi AU - Liu Y AD - Department of Reproductive and Genetics, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China. FAU - Zhao, Li AU - Zhao L AUID- ORCID: 0009-0001-5751-2811 AD - Department of Reproductive and Genetics, The First Affiliated Hospital of Kunming Medical University, Kunming 650032, China. LA - eng GR - 535 Talent Project of First Affiliated Hospital of Kunming Medical University (2024535Q02)/First Affiliated Hospital of Kunming Medical University/ GR - Doctoral Research Fund of the First Affiliated Hospital of Kunming Medical University (2021BS014)/First Affiliated Hospital of Kunming Medical University/ GR - the 535 Talent Project of the First Affiliated Hospital of Kunming Medical University (Grant No. 2023535Q12)/First Affiliated Hospital of Kunming Medical University/ PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Sphingolipids) RN - 26993-30-6 (sphingosine 1-phosphate) RN - NGZ37HRE42 (Sphingosine) RN - 0 (Lysophospholipids) RN - 0 (Ceramides) SB - IM MH - Humans MH - *Diabetes Mellitus, Type 2/metabolism/complications MH - *Sphingolipids/metabolism MH - Animals MH - Sphingosine/analogs & derivatives/metabolism MH - Lysophospholipids/metabolism MH - Ceramides/metabolism MH - Signal Transduction MH - *Diabetic Angiopathies/metabolism PMC - PMC13704962 OTO - NOTNLM OT - T2DM OT - ceramide OT - glucolipotoxicity OT - sphingolipid OT - sphingosine-1-phosphate COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/12 CRDT- 2026/10/27 10:40 PHST- 2026/09/12 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - ijms27209043 [pii] AID - ijms-27-09043 [pii] AID - 10.3390/ijms27209043 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 12;27(20):9043. doi: 10.3390/ijms27209043. PMID- 42941813 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 12 TI - Functionally Characterized Platelet-Rich Plasma Promotes Endothelial Migration and Cutaneous Wound Repair. LID - 10.3390/ijms27209041 [doi] LID - 9041 AB - Platelet-rich plasma (PRP) has been widely investigated as an autologous therapeutic for tissue regeneration; however, its clinical efficacy remains inconsistent, partly due to variability in PRP preparation protocols. In this study, we established an integrated framework for functional characterization of PRP by integrating platelet recovery, functional integrity, growth factor (GF) release, and regenerative outcomes. PRP prepared using a dual-spin centrifugation protocol at 92x g showed significantly higher platelet recovery while preserving functional responsiveness compared to single-spin conditions. PDGF-BB release increased in a time-dependent manner, peaking at 4 h after activation, and was positively correlated with platelet concentration. In an in vitro HUVEC scratch assay, PRP significantly enhanced endothelial cell migration in a platelet concentration-dependent manner. This migratory response was accompanied by increased phosphorylation of ERK and AKT and reduced VE-cadherin expression, indicating modulation of intracellular signaling pathways associated with endothelial cell growth, survival, and migration. In a rat skin wound model, PRP prepared under the selected experimental conditions accelerated wound closure compared with controls. Histological and immunohistochemical analyses revealed enhanced re-epithelialization, increased collagen deposition, increased epithelial cell proliferation, and reduced CD31-positive vascular area, consistent with accelerated vascular remodeling. Taken together, these findings demonstrate that PRP efficacy is governed by multiple interdependent factors. A comprehensive strategy integrating platelet recovery, functional preservation, GF release kinetics, and in vivo and in vitro outcomes provides a rational framework for functional characterization of PRP and may serve as a basis for subsequent translational validation. FAU - Kim, Sanggu AU - Kim S AUID- ORCID: 0000-0002-1030-5655 AD - College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea. FAU - Yang, Seongmo AU - Yang S AUID- ORCID: 0009-0006-6838-7442 AD - College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea. FAU - Kim, Soochong AU - Kim S AUID- ORCID: 0000-0002-6739-9787 AD - College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea. LA - eng GR - RS-2026-25494432/National Research Foundation of Korea/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - *Platelet-Rich Plasma/metabolism MH - Animals MH - *Cell Movement MH - *Wound Healing MH - Humans MH - Rats MH - *Skin/injuries/metabolism/pathology MH - Human Umbilical Vein Endothelial Cells MH - Male MH - Cell Proliferation MH - *Endothelial Cells/metabolism MH - Rats, Sprague-Dawley PMC - PMC13705036 OTO - NOTNLM OT - HUVEC OT - growth factors OT - platelet function OT - platelet-rich plasma OT - skin wound model COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/12 CRDT- 2026/10/27 10:40 PHST- 2026/09/07 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - ijms27209041 [pii] AID - ijms-27-09041 [pii] AID - 10.3390/ijms27209041 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 12;27(20):9041. doi: 10.3390/ijms27209041. PMID- 42941808 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 12 TI - Postmortem Gene Expression and the Thanatotranscriptome: Postmortem Interval Estimation, Forensic Applications, and Future Perspectives. LID - 10.3390/ijms27209036 [doi] LID - 9036 AB - Postmortem molecular changes are more complex than a passive, uniform cessation of all cellular activity. Human and animal studies have demonstrated that certain RNA transcripts remain detectable for defined periods after death and that transcript levels exhibit tissue- and time-dependent changes. These postmortem RNA dynamics underpin the concept of the thanatotranscriptome and may provide molecular information relevant to postmortem interval (PMI) estimation. Postmortem changes have been reported in the abundance of transcripts associated with apoptosis, cellular stress, immune responses, and DNA damage response and repair pathways. However, observed transcript levels may reflect the combined effects of antemortem RNA stability, selective RNA degradation, and potentially residual transcriptional activity. A longitudinal study of human blood reported a root mean square error (RMSE) of 4.75 h for a PMI estimation model based on multiple transcripts, illustrating the quantitative potential of this approach. Nevertheless, RNA profiles may be influenced by tissue type, temperature, cause of death and agonal processes, interindividual biological variability, and sampling and storage conditions. Methodological heterogeneity and the lack of independent external validation further limit the translation of these approaches into routine forensic practice. This review critically evaluates the molecular basis of postmortem gene expression and RNA dynamics, messenger RNA (mRNA) and microRNA (miRNA) biomarkers, transcriptome-based models for PMI estimation, and their potential forensic applications, with particular emphasis on the need for standardization, independent validation, and multi-biomarker approaches. FAU - Akpinar, Ozlem AU - Akpinar O AUID- ORCID: 0000-0003-0283-1994 AD - Eskisehir Branch Directorate, Council of Forensic Medicine, Eskisehir 26030, Turkiye. FAU - Erdem, Ziyaettin AU - Erdem Z AD - Eskisehir Branch Directorate, Council of Forensic Medicine, Eskisehir 26030, Turkiye. FAU - Keskiner, Fettah Zeki AU - Keskiner FZ AD - Adana Group Directorate, Council of Forensic Medicine, Adana 01060, Turkiye. FAU - Kaya, Kenan AU - Kaya K AUID- ORCID: 0000-0002-4157-2262 AD - Department of Forensic Medicine, Cukurova University, Adana 01330, Turkiye. LA - eng PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (RNA, Messenger) RN - 0 (MicroRNAs) RN - 0 (Biomarkers) SB - IM MH - Humans MH - *Postmortem Changes MH - Animals MH - *Transcriptome MH - RNA, Messenger/genetics/metabolism MH - MicroRNAs/genetics MH - Gene Expression Profiling MH - Biomarkers MH - RNA Stability PMC - PMC13704765 OTO - NOTNLM OT - RNA degradation OT - forensic medicine OT - forensic pathology OT - mRNA OT - miRNA OT - postmortem gene expression OT - postmortem interval OT - thanatotranscriptome OT - transcriptomics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/12 CRDT- 2026/10/27 10:40 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - ijms27209036 [pii] AID - ijms-27-09036 [pii] AID - 10.3390/ijms27209036 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 12;27(20):9036. doi: 10.3390/ijms27209036. PMID- 42941807 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 12 TI - Preclinical Mouse Models of Rheumatoid Arthritis: Comparative Insights into Inflammatory Mediators and Immune Cells. LID - 10.3390/ijms27209037 [doi] LID - 9037 AB - Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by persistent synovial inflammation, progressive cartilage destruction, and bone erosion. Mouse models are essential for investigating RA pathogenesis, defining immune-cell and cytokine networks, and evaluating potential therapies. Common models include collagen-induced arthritis (CIA), collagen antibody-induced arthritis (CAIA), human TNF transgenic (hTNF-Tg) arthritis, and SKG arthritis, each representing distinct inflammatory mechanisms. CIA involves adaptive and innate immune cells, including CD4(+) T cells, B cells, dendritic cells, macrophages, and neutrophils, with prominent roles for TNF-alpha, IL-1beta, IL-6, IL-17A, GM-CSF, and IL-23. CAIA primarily reflects antibody-mediated and innate immune mechanisms involving neutrophils, macrophages, complement, and Fcgamma receptors, with contributions from TNF-alpha, IL-1beta, IL-6, GM-CSF, CXCL1, and CXCL2. The hTNF-Tg model highlights chronic TNF-driven inflammation involving macrophages and neutrophils and downstream inflammatory mediators such as IL-1beta, IL-6, CXCL1, and CCL2. In contrast, the SKG model emphasizes autoreactive CD4(+) T-cell and Th17 responses, with IL-17A, IL-6, and IL-23 contributing to arthritis following environmental stimulation. Collectively, these models are mechanistically complementary and provide distinct insights into immune activation, cytokine signaling, and joint pathology. However, differences in immune regulation, disease heterogeneity, and inflammatory mechanisms between mice and humans emphasize the importance of careful model selection and interpretation. This review summarizes major mouse models of RA, focusing on their immune-cell and inflammatory mediator networks, therapeutic applications, and translational relevance to human disease. FAU - Lubamba, Bob Arthur AU - Lubamba BA AD - Inotiv Boulder, 5541 Central Ave. Suite #160, Boulder, CO 80301, USA. LA - eng PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Inflammation Mediators) RN - 0 (Cytokines) SB - IM MH - Animals MH - *Arthritis, Rheumatoid/immunology/pathology/metabolism/etiology MH - Disease Models, Animal MH - Humans MH - Mice MH - *Inflammation Mediators/metabolism/immunology MH - Cytokines/metabolism MH - *Arthritis, Experimental/immunology/pathology PMC - PMC13704754 OTO - NOTNLM OT - CAIA OT - CIA OT - SKG OT - human TNF transgenic OT - mouse model OT - rheumatoid arthritis COIS- Bob A. Lubamba is employed by Inotiv Boulder. The author declares that the research was conducted without any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/12 CRDT- 2026/10/27 10:40 PHST- 2026/08/14 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - ijms27209037 [pii] AID - ijms-27-09037 [pii] AID - 10.3390/ijms27209037 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 12;27(20):9037. doi: 10.3390/ijms27209037. PMID- 42941806 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 12 TI - From Prediction to Generation: Artificial Intelligence Reconstructing the Paradigm of Protein Glycosylation Research. LID - 10.3390/ijms27209032 [doi] LID - 9032 AB - Among post-translational modifications, protein glycosylation is distinguished by its abundance and structural intricacy. Unlike protein biosynthesis, glycan assembly follows no template; it reflects glycosyltransferase/glycosidase competition, sugar-donor availability, and organelle-enforced compartmentalization, yielding heterogeneous glycoforms that confer functional versatility but complicate analysis. Three bottlenecks persist: stereochemical control in chemical glycosylation, ambiguous assignment of occupancy sites, and reproducing native conformations of fully glycosylated proteins. Adopting the "glycan language" framework, we survey how AI addresses each, emphasizing the systems on which it has been tested. In synthesis planning, GlycoPredictor uncovered anomeric-selectivity rules such as the anti-nitrile and C2-azido alpha-directing effects, while Bayesian optimization discovered lithium-salt-directed stereoselective glycosylation; for site identification, ESM-2 with AlphaFold2-derived structural features improves N- and O-glycosite prediction, and DeepGlycanSite maps carbohydrate-binding interfaces. For structure and dynamics, AlphaFold3 and multimodal cryo-electron microscopy (cryo-EM), hydrogen-deuterium exchange mass spectrometry, and molecular dynamics (MD) modeling move glycoproteins toward conformational ensembles, as in the AI-assisted dissection of the MCTS1-JAK2-IFN-gamma axis. In glycomics, deep learning resolves intact glycopeptides and de novo glycan topology and has defined Fc N-glycan signatures of immunoglobulin G in autoimmune and malignant disease. Because glycan language models link sequence and structure to function, they are becoming clinically actionable: they prioritize candidate variants in congenital disorders of glycosylation and stratify immune disorders and tumors by aberrant glycosylation. Remaining obstacles include scarce structure-linked datasets, encoding of branched topologies, multimodal fusion, and interpretability. FAU - Zhu, Junhong AU - Zhu J AUID- ORCID: 0009-0004-0945-1489 AD - Center for Medical Genetics, West China Second University Hospital, Sichuan University, Chengdu 610041, China. AD - Key Laboratory of Birth Defects and Related Diseases of Women and Children, Sichuan University, Ministry of Education, Chengdu 610041, China. AD - West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China. FAU - Huang, Shuang AU - Huang S AD - Center for Medical Genetics, West China Second University Hospital, Sichuan University, Chengdu 610041, China. AD - Key Laboratory of Birth Defects and Related Diseases of Women and Children, Sichuan University, Ministry of Education, Chengdu 610041, China. FAU - Zhang, Nannan AU - Zhang N AD - Key Laboratory of Birth Defects and Related Diseases of Women and Children, Sichuan University, Ministry of Education, Chengdu 610041, China. AD - National Center for Birth Defect Monitoring, West China Second University Hospital, Sichuan University, Chengdu 610041, China. AD - State Key Laboratory of Biotherapy, Collaborative Innovation Center of Biotherapy, Sichuan University, Chengdu 610041, China. LA - eng GR - 2024NSFSC0599/The Natural Science Foundation Project of Sichuan/ GR - 2024SCUQJTX037/The Young Teachers' Science and Technology Innovation Capability Enhancement Project of Sichuan University/ PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Glycoproteins) RN - 0 (Polysaccharides) SB - IM MH - Glycosylation MH - *Artificial Intelligence MH - *Protein Processing, Post-Translational MH - Humans MH - *Glycoproteins/chemistry/metabolism MH - Protein Conformation MH - Polysaccharides/chemistry/metabolism PMC - PMC13705451 OTO - NOTNLM OT - artificial intelligence OT - glycan language OT - glycoengineering OT - glycoprotein structure OT - glycosite prediction OT - protein glycosylation OT - protein language model COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/12 CRDT- 2026/10/27 10:40 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - ijms27209032 [pii] AID - ijms-27-09032 [pii] AID - 10.3390/ijms27209032 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 12;27(20):9032. doi: 10.3390/ijms27209032. PMID- 42941801 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 12 TI - Architect or Destroyer: The Diversity of Glucocorticoid Receptor Signaling Pathways in the Brain. LID - 10.3390/ijms27209027 [doi] LID - 9027 AB - Glucocorticoids (GCs), acting through the glucocorticoid (GR) and mineralocorticoid (MR) receptors, are essential for brain development, stress adaptation, and metabolic homeostasis, yet their signaling exhibits context-dependent diversity yielding either beneficial or detrimental outcomes. This review synthesizes current knowledge on the multifaceted mechanisms through which GR integrates hormonal, metabolic, and stress signals to modulate transcriptional programs in the brain. We highlight three key levels of GR regulatory complexity: the diversity of DNA GR-binding motifs, GR-mediated epigenetic remodeling, and rapid non-genomic cascades. Clinical and experimental data reveal that while physiological GC levels are critical for normal maturation and function, excess or chronic exposure, especially during sensitive developmental windows, can disrupt neurogenesis, synaptogenesis, and myelination, predisposing individuals to cognitive, affective, and neurodegenerative disorders. We discuss promising therapeutic strategies based on selective modulation of MR/GR balance, interference with specific co-regulatory complexes, and targeted manipulation of DNMT/TET/HDAC cascades. Unlike most existing reviews-which address either (a) the MR-GR interaction as a whole receptor system, (b) non-genomic effects in isolation from genomic ones, or (c) the clinical manifestations of stress-associated disorders without an in-depth mechanistic analysis of GR targets-our manuscript attempts to integrate these three levels of GR regulation into a unified hierarchical system (binding motifs --> epigenetic remodeling --> the conditional nature of the boundary between genomic and non-genomic GR effects). We aim to explicitly demonstrate how these levels jointly determine cell-specific outcomes, rather than describing them in parallel as independent mechanisms. However, critical gaps remain in understanding the precise assembly of GR-co-regulator complexes in defined neuronal and glial populations, as well as the feasibility of selectively engaging beneficial GR signaling branches without systemic suppression of the hypothalamic-pituitary-adrenal axis. Addressing these questions is essential for developing next-generation therapies that harness GR's architectural potential while avoiding its adverse or unwanted consequences. FAU - Shkarina, Liliia AU - Shkarina L AUID- ORCID: 0009-0005-1774-3534 AD - Medical Research and Education Institute, Lomonosov Moscow State University, 27/10, Lomonosovsky Ave., 119192 Moscow, Russia. FAU - Khaybullina, Raushana AU - Khaybullina R AD - Medical Research and Education Institute, Lomonosov Moscow State University, 27/10, Lomonosovsky Ave., 119192 Moscow, Russia. FAU - Primak, Alexandra AU - Primak A AUID- ORCID: 0000-0002-1386-5922 AD - Medical Research and Education Institute, Lomonosov Moscow State University, 27/10, Lomonosovsky Ave., 119192 Moscow, Russia. FAU - Bozov, Kirill AU - Bozov K AD - Medical Research and Education Institute, Lomonosov Moscow State University, 27/10, Lomonosovsky Ave., 119192 Moscow, Russia. FAU - Dzhauari, Stalik AU - Dzhauari S AD - Medical Research and Education Institute, Lomonosov Moscow State University, 27/10, Lomonosovsky Ave., 119192 Moscow, Russia. FAU - Karagyaur, Maxim AU - Karagyaur M AUID- ORCID: 0000-0003-4289-3428 AD - Medical Research and Education Institute, Lomonosov Moscow State University, 27/10, Lomonosovsky Ave., 119192 Moscow, Russia. LA - eng GR - 22-15-00125-Pi/Russian Science Foundation/ PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Receptors, Glucocorticoid) RN - 0 (Glucocorticoids) RN - 0 (Receptors, Mineralocorticoid) SB - IM MH - Humans MH - *Receptors, Glucocorticoid/metabolism/genetics MH - *Signal Transduction MH - Animals MH - *Brain/metabolism MH - Epigenesis, Genetic MH - Glucocorticoids/metabolism MH - Receptors, Mineralocorticoid/metabolism PMC - PMC13704554 OTO - NOTNLM OT - brain tissue OT - epigenetics OT - glucocorticoid receptor OT - mental disorders OT - signaling pathways OT - stress COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/12 CRDT- 2026/10/27 10:40 PHST- 2026/08/25 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - ijms27209027 [pii] AID - ijms-27-09027 [pii] AID - 10.3390/ijms27209027 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 12;27(20):9027. doi: 10.3390/ijms27209027. PMID- 42941798 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 12 TI - Sexually Dimorphic Glutamatergic Control of R-Spondin 1 and R-Spondin 3 Neurons in Hypothalamic Reproductive and Metabolic Nuclei. LID - 10.3390/ijms27209026 [doi] LID - 9026 AB - R-spondin 1 (RSPO1) and R-spondin 3 (RSPO3) contribute to reproductive development and feeding regulation, but their hypothalamic glutamatergic regulation remains unclear. We hypothesized that NMDA and non-NMDA receptor mechanisms contribute to hypothalamic RSPO neuronal activation. Given steroid-sensitive hypothalamic glutamatergic signaling, we compared activation and antagonist-mediated attenuation between sexes. Adult male and metestrous female Sprague-Dawley rats received kainic acid or AMPA with or without CNQX pretreatment, or NMDA with or without MK-801 pretreatment. RSPO/c-Fos labeling assessed activation in the supraoptic (SON), paraventricular (PVN), and arcuate (ARC) nuclei; ELISA assessed accompanying changes in serum RSPO concentrations. All agonists activated both populations in all nuclei. Only kainate-induced PVN RSPO3 activation differed between sexes (females, 89.3%; males, 72.8%; unadjusted p = 0.039), an isolated finding among 18 agonist comparisons. Antagonists attenuated all responses, although activation remained above control in 17 of 36 conditions. Females showed greater attenuation in selected kainate and NMDA conditions in the SON and PVN. Serum changes did not parallel neuronal activation. These findings support NMDA and non-NMDA receptor involvement and sex-dependent attenuation of RSPO neuronal activation. Given the central feeding effects of RSPOs, their sex-dependent glutamatergic regulation within neuroendocrine nuclei may contribute to sex differences in metabolic control. FAU - Topal, Gonca AU - Topal G AD - Department of Histology and Embryology, Faculty of Medicine, Bursa Uludag University, Bursa 16059, Turkiye. FAU - Yurtseven, Duygu Gok AU - Yurtseven DG AUID- ORCID: 0000-0003-4969-3584 AD - Department of Histology and Embryology, Faculty of Medicine, Bursa Uludag University, Bursa 16059, Turkiye. FAU - Akbulut, Nursel Hasanoglu AU - Akbulut NH AD - Department of Histology and Embryology, Faculty of Medicine, Canakkale Onsekiz Mart University, Canakkale 17020, Turkiye. FAU - Gul, Zulfiye AU - Gul Z AD - Department of Medical Pharmacology, Faculty of Medicine, Dokuz Eylul University, Izmir 35340, Turkiye. FAU - Udum, Duygu AU - Udum D AD - Department of Biochemistry, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa 16059, Turkiye. FAU - Yalcin, Murat AU - Yalcin M AD - Department of Veterinary Physiology, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa 16059, Turkiye. FAU - Eyigor, Ozhan AU - Eyigor O AUID- ORCID: 0000-0003-3463-7483 AD - Department of Histology and Embryology, Faculty of Medicine, Bursa Uludag University, Bursa 16059, Turkiye. LA - eng GR - 3501-123S881/Turkiye Bilimsel ve Teknolojik Arastirma Kurumu/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Thrombospondins) RN - 0 (R-Spondins) RN - 0 (RLN3 protein, rat) RN - 3KX376GY7L (Glutamic Acid) RN - 0 (Nerve Tissue Proteins) RN - 9002-69-1 (Relaxin) SB - IM MH - Animals MH - Female MH - Male MH - *Neurons/metabolism/drug effects MH - *Thrombospondins/metabolism MH - Rats MH - R-Spondins MH - Rats, Sprague-Dawley MH - *Hypothalamus/metabolism MH - Sex Characteristics MH - *Glutamic Acid/metabolism MH - Nerve Tissue Proteins MH - Relaxin PMC - PMC13705108 OTO - NOTNLM OT - LGR4 OT - R-Spondin 1 OT - R-spondin 3 OT - WNT/beta-catenin OT - c-Fos OT - glutamate receptors OT - hypothalamus OT - paraventricular nucleus OT - reproduction OT - sexual dimorphism COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/12 CRDT- 2026/10/27 10:40 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - ijms27209026 [pii] AID - ijms-27-09026 [pii] AID - 10.3390/ijms27209026 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 12;27(20):9026. doi: 10.3390/ijms27209026. PMID- 42941792 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 12 TI - Molecular Dynamics-Guided Triage of Approved Drugs Against Escherichia coli Resistance Targets Nominates Capmatinib, Metergoline and Nilotinib as Candidates for Experimental Testing. LID - 10.3390/ijms27209017 [doi] LID - 9017 AB - Virtual screening ranks compounds by scoring functions that correlate with molecular size, and hit lists are rarely benchmarked against compounds of established activity. We screened 2070 approved drugs against four Escherichia coli targets selected by pocket-druggability triage (FabI, LpxC, DHFR and GyrB), with each docking protocol validated by redocking its native ligand (RMSD 0.50-0.69 A). Per target, the two top-scoring compounds and a reference inhibitor were simulated for 100 ns in triplicate (12 complexes, 3.6 mus). Docking did not recover the references: trimethoprim ranked 762/2068 and novobiocin 576/2069, while top-ranked compounds were large, promiscuous drugs recurring across unrelated targets. Pose triage failed, too: all twelve complexes retained 43-87% of initial contacts, contact-loss time separated targets, not compounds, and MM-GBSA favoured the reference at one target of four. Persistent hydrogen bonding separated the reference from the hits at three of four targets, by 2-9-fold, although the GyrB result rests largely on one replicate. Benchmarking each hit against its reference nominates three candidates for experimental testing: capmatinib at DHFR, metergoline at FabI and nilotinib at LpxC. Capmatinib ranks highest on these computational criteria, with the highest pocket retention (87%) and an MM-GBSA energy with no detectable difference from trimethoprim's at n = 3 (absence of evidence for a difference, not evidence of equivalence). In this small panel, carrying a known active through the same pipeline was therefore more informative than docking score, pose stability or end-point rescoring. The nominations are hypotheses: no experimental validation was performed, and generality would require multiple actives and property-matched decoys per target. FAU - Abdelsayed, Mena AU - Abdelsayed M AD - Lankenau Institute for Medical Research, Philadelphia, PA 19096, USA. FAU - Boulaamane, Yassir AU - Boulaamane Y AUID- ORCID: 0000-0002-2939-7772 AD - Laboratory of Innovative Technologies, National School of Applied Sciences of Tangier, Abdelmalek Essaadi University, Tetouan 93000, Morocco. LA - eng PT - Journal Article DEP - 20261012 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Pyrimidines) RN - F41401512X (nilotinib) RN - 0 (Triazines) RN - 0 (Anti-Bacterial Agents) RN - EC 1.5.1.3 (Tetrahydrofolate Dehydrogenase) RN - 0 (Escherichia coli Proteins) RN - 0 (Ligands) RN - 0 (Imidazoles) SB - IM MH - *Pyrimidines/pharmacology/chemistry MH - *Escherichia coli/drug effects MH - Molecular Docking Simulation MH - *Molecular Dynamics Simulation MH - *Triazines/pharmacology/chemistry MH - *Anti-Bacterial Agents/pharmacology/chemistry MH - Tetrahydrofolate Dehydrogenase/chemistry/metabolism MH - *Drug Resistance, Bacterial/drug effects MH - Escherichia coli Proteins/chemistry/metabolism/antagonists & inhibitors MH - Ligands MH - *Imidazoles/pharmacology/chemistry PMC - PMC13705101 OTO - NOTNLM OT - FabI OT - LpxC OT - antimicrobial resistance OT - capmatinib OT - dihydrofolate reductase OT - drug repurposing OT - hydrogen bonding OT - molecular docking OT - molecular dynamics OT - scoring-function bias COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/12 CRDT- 2026/10/27 10:40 PHST- 2026/09/20 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - ijms27209017 [pii] AID - ijms-27-09017 [pii] AID - 10.3390/ijms27209017 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 12;27(20):9017. doi: 10.3390/ijms27209017. PMID- 42941790 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 11 TI - Green Micelle-Enhanced Spectrofluorimetric Determination of Gefitinib in Pharmaceutical Tablets Using Cremophor RH 40: Response-Surface Optimization and Time-Dependent Density Functional Theory (TD-DFT) Interpretation. LID - 10.3390/ijms27209015 [doi] LID - 9015 AB - Gefitinib is an epidermal growth factor receptor inhibitor used in non-small-cell lung cancer. This study developed a micelle-enhanced spectrofluorimetric method for its determination in tablets using native fluorescence and Cremophor RH 40 without derivatization. Experimental conditions were screened by a univariate approach and further evaluated by response-surface methodology. The quadratic model was significant (F = 83.26, p = 0.0019), and three confirmation experiments yielded prediction errors of 0.515-0.954%, supporting local agreement at the selected conditions. Fluorescence intensity was measured at an emission wavelength of 394 nm following excitation at 333 nm. The method was linear over 50-1200 ng mL(-1) (r = 0.9997). The calculated limits of detection and quantification were 6.89 and 20.89 ng mL(-1), respectively, while 50 ng mL(-1) was retained as the lowest validated concentration. The method showed acceptable accuracy, repeatability, and selectivity for the investigated tablet matrix. Robustness testing indicated that pH requires careful control around 8.0. Analysis of pooled commercial tablets yielded an assay of 100.21 +/- 1.86% of the label claim. TD-DFT calculations supported qualitative interpretation of the electronic transitions. The AGREE score was 0.61, indicating a more favorable environmental profile than the reference spectrofluorimetric method comparator (0.46). FAU - Bakheit, Ahmed H AU - Bakheit AH AUID- ORCID: 0000-0002-0445-1357 AD - Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia. FAU - Alzaid, Abdullah M A AU - Alzaid AMA AD - Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia. FAU - Almajed, Abdulrahman A AU - Almajed AA AD - Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia. LA - eng GR - King Saud University/ PT - Journal Article DEP - 20261011 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - S65743JHBS (Gefitinib) RN - 0 (Micelles) RN - 0 (Tablets) RN - PDC6A3C0OX (Glycerol) SB - IM MH - *Gefitinib/analysis/chemistry MH - *Micelles MH - Spectrometry, Fluorescence/methods MH - *Tablets/chemistry MH - *Glycerol/chemistry/analogs & derivatives MH - Reproducibility of Results MH - Limit of Detection PMC - PMC13704709 OTO - NOTNLM OT - Cremophor RH 40 OT - TD-DFT OT - gefitinib OT - green analytical chemistry OT - micelle-enhanced spectrofluorimetry OT - pharmaceutical analysis COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/11 CRDT- 2026/10/27 10:40 PHST- 2026/09/08 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - ijms27209015 [pii] AID - ijms-27-09015 [pii] AID - 10.3390/ijms27209015 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 11;27(20):9015. doi: 10.3390/ijms27209015. PMID- 42941788 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 11 TI - Relation-Aware Heterogeneous Graph Learning for miRNA-Drug Response Association Prediction Under Cold-Start Generalization. LID - 10.3390/ijms27209013 [doi] LID - 9013 AB - (1) Background: Cancer drug resistance can be influenced by microRNAs (miRNAs), which can alter gene expression and cellular pathways that determine how cancer cells respond to treatment. Potential miRNA-drug response associations can be identified using computational models; however, evaluating these models using edge-based evaluation can result in an optimistic view of model generalization since test miRNAs or drugs can appear in other response associations during training. (2) Methods: This paper presents a relation-aware heterogeneous learning framework that integrates miRNA expression profiles, miRNA-gene regulatory interactions, gene-gene functional associations, drug molecular features, and distinct drug-gene relations in a unified miRNA-gene-drug network. A Heterogeneous Graph Transformer (HGT) was used to preserve relation-specific information, and biological paths connecting each miRNA to each drug were used as additional information for the corresponding candidate miRNA-drug pair. A leakage-safe five-fold cross-validation approach was used to evaluate the proposed framework under different settings: edge, miRNA-cold, drug-cold, and double-cold. (3) Results: The proposed framework obtained an AUC and AUPR of 0.9537 +/- 0.0044 and 0.9494 +/- 0.0069, respectively, under the edge-based setting. The performance remained high under the miRNA-cold-based setting, where the model achieved an AUC and AUPR of 0.9466 +/- 0.0061 and 0.9436 +/- 0.0049, respectively. A substantial drop in performance was observed under the drug-cold setting, where the proposed framework obtained an AUC and AUPR of 0.6685 +/- 0.0354 and 0.6534 +/- 0.0569, respectively. The performance was also substantially reduced under the double-cold-based setting. The results also show that the structural resemblance alone is not enough to explain generalization to new drugs, since no significant relationship between cold-start performance and either ChemBERTa molecular similarity or DrugBank target similarity was shown by drug-level analysis. Literature evidence supported 6 out of 20 top-ranked previously unlabeled predictions compared to 1 of 120 randomly selected controls (Fisher's exact test, p = 2.63 x 10(-5); empirical p = 2.80 x 10(-5)). (4) Conclusions: The obtained results show that the proposed framework can rank candidate miRNA-drug response associations effectively. They also show that predicting response associations under the drug-cold-based setting remains much harder than predicting for similarly held-out miRNAs, which is an important limitation of conventional edge-based evaluation. FAU - Elbashir, Murtada K AU - Elbashir MK AUID- ORCID: 0000-0003-3431-6604 AD - Department of Information Systems, College of Computer and Information Sciences, Jouf University, Sakaka 72441, Saudi Arabia. LA - eng GR - DGSSR-2025-02- 01163/Jouf University/ PT - Journal Article DEP - 20261011 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (MicroRNAs) RN - 0 (Antineoplastic Agents) SB - IM MH - *MicroRNAs/genetics/metabolism MH - Humans MH - Gene Regulatory Networks MH - *Computational Biology/methods MH - *Antineoplastic Agents/pharmacology/therapeutic use MH - *Drug Resistance, Neoplasm/genetics MH - *Neoplasms/genetics/drug therapy MH - Algorithms MH - Gene Expression Regulation, Neoplastic/drug effects PMC - PMC13704664 OTO - NOTNLM OT - cancer drug resistance OT - cold-start prediction OT - heterogeneous graph learning OT - miRNA-drug response association COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/11 CRDT- 2026/10/27 10:40 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - ijms27209013 [pii] AID - ijms-27-09013 [pii] AID - 10.3390/ijms27209013 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 11;27(20):9013. doi: 10.3390/ijms27209013. PMID- 42941786 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 11 TI - Plasma Lipidomic Phenotype Associated with Pathogenic LDLR Variants in Familial Hypercholesterolemia. LID - 10.3390/ijms27209011 [doi] LID - 9011 AB - Familial hypercholesterolemia (FH) is caused by an impaired ability to clear circulating low-density lipoprotein (LDL-C) particles due to a defective low-density lipoprotein receptor (LDLR). FH significantly increases cardiovascular risk (CVR). Although conventional lipid parameters are essential for diagnosing FH, they do not fully capture the metabolic impact of inherited LDLR dysfunction. This study aimed to characterize the plasma lipidomic phenotype associated with pathogenic LDLR variants in individuals with suspected FH. Forty-four participants underwent genetic testing for LDLR, apolipoprotein B-100 (APOB), and protein convertase subtilisin/kexin type 9 (PCSK9). After excluding three carriers of the APOB p.Arg3527Gln variant, the comparative lipidomic analysis included 41 participants. The study included 17 carriers of pathogenic LDLR variants and 24 participants without a detected pathogenic variant in the tested panel. We performed plasma lipidomic profiling using flow-injection analysis coupled with high-resolution tandem mass spectrometry (FIA-MS/MSALL) without chromatographic separation. Lipid annotation and data processing were performed using LipidView software, and lipidomic measurements were expressed as relative signal abundances derived from mass spectrometry (MS) peak intensities. Statistical analyses included Mann-Whitney U tests with false discovery rate (FDR) correction across the analyzed lipid features. Exploratory multivariable logistic regression and receiver operating characteristic (ROC) analyses were also performed. There were no differences in conventional lipid parameters between genetically confirmed LDLR variant carriers and participants without a detected pathogenic variant in the tested panel. At the nominal level (unadjusted p < 0.05), lipidomic profiling revealed differences in glycerophospholipid (GP) related analytical features between groups. However, no individual lipid feature remained statistically significant after full-family FDR correction. Among (GPs), 69 feature records (16.3%) showed nominal between-group differences, predominantly lower abundance in LDLR variant carriers. The most consistent nominal alterations were observed in features annotated as phosphatidylinositol triphosphates (PIP3) and N-acyl phosphatidylethanolamines (NAPE). Among the analyzed fatty acyls (FAs), 72.7% showed nominally lower relative abundance in LDLR variant carriers. We used exploratory multivariable models to identify GP and FA-related features associated with the detected pathogenic LDLR variant status. The models showed within-cohort discrimination. However, these models were developed and evaluated in the same small cohort after data-driven variable selection. They were not externally validated. Therefore, the findings should be interpreted as hypothesis-generating and do not establish diagnostic performance, clinical utility, or functional pathway impairment. Further validation in larger cohorts using targeted lipidomics with chromatographic separation is necessary. FAU - Was, Joanna AU - Was J AUID- ORCID: 0000-0002-4904-6398 AD - Department of Medical Biology, National Institute of Cardiology, State Research Institute, 04-628 Warsaw, Poland. FAU - Prejbisz, Aleksander AU - Prejbisz A AD - Department of Epidemiology, Cardiovascular Disease Prevention and Health Promotion, National Institute of Cardiology, State Research Institute, 04-628 Warsaw, Poland. FAU - Niedolistek, Magdalena AU - Niedolistek M AD - Department of Medical Biology, National Institute of Cardiology, State Research Institute, 04-628 Warsaw, Poland. FAU - Kowalik, Ilona AU - Kowalik I AUID- ORCID: 0000-0002-8040-5109 AD - Clinical Research Support Centre, National Institute of Cardiology-State Research Institute, 04-628 Warsaw, Poland. FAU - Minota, Ilona AU - Minota I AD - Department of Medical Biology, National Institute of Cardiology, State Research Institute, 04-628 Warsaw, Poland. FAU - Poninska, Joanna AU - Poninska J AUID- ORCID: 0000-0002-0890-611X AD - Department of Medical Biology, National Institute of Cardiology, State Research Institute, 04-628 Warsaw, Poland. FAU - Drohomirecka, Anna AU - Drohomirecka A AUID- ORCID: 0000-0002-0998-4291 AD - Heart Failure and Transplantology Clinic, National Institute of Cardiology-State Research Institute, 04-628 Warsaw, Poland. FAU - Sokolowska, Dorota AU - Sokolowska D AD - Department of Medical Biology, National Institute of Cardiology, State Research Institute, 04-628 Warsaw, Poland. FAU - Ploski, Rafal AU - Ploski R AUID- ORCID: 0000-0001-6286-5526 AD - Department of Medical Biology, National Institute of Cardiology, State Research Institute, 04-628 Warsaw, Poland. AD - Department of Medical Genetics, Medical University of Warsaw, 02-106 Warsaw, Poland. FAU - Dobrowolski, Piotr AU - Dobrowolski P AD - Department of Epidemiology, Cardiovascular Disease Prevention and Health Promotion, National Institute of Cardiology, State Research Institute, 04-628 Warsaw, Poland. LA - eng GR - The project was financed by the National Science Centre in Krakow, Poland. Scientific activity no. 2020/04/X/NZ7/00104 - MINIATURE 4./National Science Centre/ PT - Journal Article DEP - 20261011 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Receptors, LDL) RN - 0 (Lipids) RN - 0 (LDLR protein, human) RN - EC 3.4.21.- (Proprotein Convertase 9) RN - 0 (Apolipoprotein B-100) SB - IM MH - Humans MH - *Hyperlipoproteinemia Type II/genetics/blood MH - *Lipidomics/methods MH - *Receptors, LDL/genetics MH - Female MH - Phenotype MH - Male MH - Middle Aged MH - *Lipids/blood MH - Adult MH - Proprotein Convertase 9/genetics MH - Apolipoprotein B-100/genetics PMC - PMC13705200 OTO - NOTNLM OT - LDLR OT - cardiovascular high risk (HCVR) OT - familial hypercholesterolemia OT - lipidomics OT - metabolomics OT - pathogenic variants COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/11 CRDT- 2026/10/27 10:40 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - ijms27209011 [pii] AID - ijms-27-09011 [pii] AID - 10.3390/ijms27209011 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 11;27(20):9011. doi: 10.3390/ijms27209011. PMID- 42941784 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 11 TI - Pyrazolate-Bridged PdCo and PdCu Complexes as Dual DNA-Binding and ROS-Driven Cytotoxic Agents. LID - 10.3390/ijms27209009 [doi] LID - 9009 AB - Heterometallic complexes offer a promising anticancer strategy owing to their simultaneous action on multiple biological targets. In this work, we report the synthesis and evaluation of the antitumor potential of two heterometallic pyrazolate-bridged complexes, [PdCo(phen)(micro-dmpz)(2)(OOCMe)(2)] (PdCo) and [PdCu(phen)(micro-dmpz)(2)(OOCMe)(2)] (PdCu) (phen = 1,10-phenanthroline, dmpz = 3,5-dimethylpyrazole). The complexes were comprehensively characterized using a combined spectroscopic and analytical approach, including single-crystal X-ray diffraction (SC-XRD), PXRD, FTIR, UV-Vis, and mass spectrometry, confirming their structural integrity in both solid state and solution. Their cytotoxicity was assessed in vitro against colon, A549 lung, and breast cancer cell lines, as well as normal human dermal fibroblasts. The complexes exhibited micromolar IC(50) values with selectivity indices up to 7. Using circular dichroism (CD) and fluorescence spectroscopy, it was demonstrated that PdCo binds to DNA, with minor groove binding (K(d) = 7.6 muM) being the preferred mode of interaction, dominating over intercalation (K(d) = 26.1 muM). Molecular docking simulations confirmed that the dissociated [Pd(phen)(micro-dmpz)(2)] fragment is capable of binding to DNA. Furthermore, chemiluminescence assays revealed pronounced peroxidase-like and superoxide dismutase-like activities of PdCu and PdCo, implicating ROS-mediated oxidative stress as a second, complementary cytotoxic pathway. Collectively, these findings highlight the dual-action mechanism of the synthesized complexes, combining DNA targeting with ROS-mediated oxidative damage, positioning them as highly potent candidates for further anticancer development. FAU - Uvarova, Marina A AU - Uvarova MA AUID- ORCID: 0000-0003-4617-8966 AD - Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Leninsky Prosp. 31, GSP-1, 119991 Moscow, Russia. FAU - Kulikova, Vitalia V AU - Kulikova VV AUID- ORCID: 0000-0003-0618-1407 AD - Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova Str., 32, 119991 Moscow, Russia. FAU - Kozmenko, Yaroslav V AU - Kozmenko YV AUID- ORCID: 0009-0004-3563-6612 AD - Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova Str., 32, 119991 Moscow, Russia. FAU - Revtovich, Svetlana V AU - Revtovich SV AUID- ORCID: 0000-0003-1594-9334 AD - Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova Str., 32, 119991 Moscow, Russia. FAU - Filippova, Arina D AU - Filippova AD AUID- ORCID: 0000-0002-2725-8891 AD - Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Leninsky Prosp. 31, GSP-1, 119991 Moscow, Russia. FAU - Shmelev, Maxim A AU - Shmelev MA AUID- ORCID: 0000-0002-7345-6856 AD - Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Leninsky Prosp. 31, GSP-1, 119991 Moscow, Russia. FAU - Sokolova, Darina V AU - Sokolova DV AUID- ORCID: 0000-0003-3972-2425 AD - N.N. Blokhin National Medical Research Center of Oncology, Kashirskoe Shosse 23, 115522 Moscow, Russia. FAU - Pokrovsky, Vadim S AU - Pokrovsky VS AUID- ORCID: 0000-0003-4006-9320 AD - N.N. Blokhin National Medical Research Center of Oncology, Kashirskoe Shosse 23, 115522 Moscow, Russia. AD - Peoples' Friendship University of Russia, Miklukho-Maklaya 6, 117198 Moscow, Russia. FAU - Kaverina, Alexandra P AU - Kaverina AP AD - Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova Str., 32, 119991 Moscow, Russia. FAU - Solyev, Pavel N AU - Solyev PN AUID- ORCID: 0000-0002-5546-4975 AD - Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova Str., 32, 119991 Moscow, Russia. FAU - Eremenko, Igor L AU - Eremenko IL AUID- ORCID: 0000-0001-6861-1404 AD - Kurnakov Institute of General and Inorganic Chemistry, Russian Academy of Sciences, Leninsky Prosp. 31, GSP-1, 119991 Moscow, Russia. LA - eng GR - 22-13-00175-P/Russian Science Foundation/ PT - Journal Article DEP - 20261011 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Reactive Oxygen Species) RN - 9007-49-2 (DNA) RN - 0 (Antineoplastic Agents) RN - 5TWQ1V240M (Palladium) RN - 789U1901C5 (Copper) RN - 0 (Coordination Complexes) RN - 0 (Pyrazoles) RN - 0 (Cytotoxins) RN - 0 (Phenanthrolines) RN - W4X6ZO7939 (1,10-phenanthroline) SB - IM MH - Humans MH - *Reactive Oxygen Species/metabolism MH - *DNA/metabolism/chemistry MH - *Antineoplastic Agents/pharmacology/chemistry MH - *Palladium/chemistry MH - *Copper/chemistry MH - *Coordination Complexes/pharmacology/chemistry/chemical synthesis MH - *Pyrazoles/chemistry/pharmacology MH - Cell Line, Tumor MH - *Cytotoxins/pharmacology/chemistry MH - Phenanthrolines/chemistry PMC - PMC13704585 OTO - NOTNLM OT - ROS OT - anticancer activity OT - crystal structure OT - docking OT - heterometallic compounds OT - palladium(II) COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/11 CRDT- 2026/10/27 10:40 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - ijms27209009 [pii] AID - ijms-27-09009 [pii] AID - 10.3390/ijms27209009 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 11;27(20):9009. doi: 10.3390/ijms27209009. PMID- 42941783 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 11 TI - Eukaryotic Mitogenomes and Their Key Insights: Architecture, Evolution, and Mitonuclear Coevolution. LID - 10.3390/ijms27209010 [doi] LID - 9010 AB - Mitochondrial genomes (mitogenomes) are no longer considered entities solely responsible for cellular bioenergetics, but as dynamic, multifaceted, and synchronized systems that integrate genome architecture, evolutionary processes, and organismal physiology. The present review summarizes the contemporary understanding of mitogenome organization and its diversity, evolution, and associated functions across eukaryotic life, with emphasis on mitonuclear co-evolution and relevance to human health. Moreover, the conserved and lineage-specific characteristics of mitogenome architecture, including its genetic content, topology, and regulatory elements, and their roles in governing the precise replication and transcription of the mitogenome are also comprehensively discussed. Additionally, the review explores the role of evolutionary dynamics, with emphasis on mutation-rate heterogeneity, recombination dynamics, gene rearrangements, and the interaction between mutation, natural selection, and genetic drift in shaping mitochondrial genome diversity and evolutionary trajectories. Comparative approaches have revealed extensive diversity in mitogenome structural organization and composition, which are closely associated with life-history traits of organisms, including metabolic rate, longevity, and reproductive characteristics. Notably, at the functional level, the variability potential of a mitogenome significantly influences oxidative phosphorylation efficiency, reactive oxygen species generation, and the orchestration of cellular signaling cascades. Fundamental to these processes is the existence of coordinated mitonuclear interrelationships, wherein coevolution between mitochondrial and nuclear-encoded genes safeguards bioenergetic functional integrity, whereas disruptions in this coordination may lead to reduced cellular fitness and progression of several diseases. Particularly in humans, mutations in mitochondrial DNA (mtDNA) and heteroplasmy significantly contribute to the development of primary mitochondrial disorders and are increasingly implicated in other complex disease etiologies, including neurodegeneration, metabolic syndromes, and cancer. The paper also summarizes the phylogenetic utility of mitogenomes in the genomic era, accentuating the need to employ integrative approaches that combine mitochondrial and nuclear data. Lastly, the role of emerging technological platforms, including long-read sequencing and multi-omics, is also discussed, since these approaches are expected to transform the current landscape of mitogenome research. Altogether, the manuscript positions mitogenomes as indispensable entities bridging the gaps between evolutionary histories, physiological functions, and personalized precision medicine. FAU - Mehrotra, Arpit AU - Mehrotra A AUID- ORCID: 0000-0002-0378-0247 AD - Department of Allied Health Sciences, Chitkara School of Health Sciences, Chitkara University, Punjab 140401, India. FAU - Sood, Abhilasha AU - Sood A AUID- ORCID: 0000-0003-4714-4493 AD - Department of Allied Health Sciences, Chitkara School of Health Sciences, Chitkara University, Punjab 140401, India. FAU - Kartavtsev, Yuri P AU - Kartavtsev YP AUID- ORCID: 0000-0002-0436-4763 AD - A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok 690041, Russia. LA - eng PT - Journal Article PT - Review DEP - 20261011 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - *Genome, Mitochondrial MH - *Evolution, Molecular MH - Humans MH - Animals MH - *Cell Nucleus/genetics MH - *Mitochondria/genetics MH - *Eukaryota/genetics PMC - PMC13705065 OTO - NOTNLM OT - Dn/Ds OT - PCGs OT - bioenergetics OT - heteroplasmy OT - mitochondrial DNA OT - mitochondrial disease OT - mitochondrial evolution OT - mitogenome architecture OT - mitogenomes OT - mitonuclear coevolution OT - mtDNA OT - nDNA OT - nuclear DNA OT - phylogenomics OT - protein-coding genes OT - substitution rates for non-synonymous and synonymous mutations (substitutions) COIS- The authors have no conflicts of interest to declare. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/11 CRDT- 2026/10/27 10:40 PHST- 2026/07/29 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - ijms27209010 [pii] AID - ijms-27-09010 [pii] AID - 10.3390/ijms27209010 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 11;27(20):9010. doi: 10.3390/ijms27209010. PMID- 42941781 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 11 TI - Integrated Single-Cell and Transcriptomic Analysis Suggests a Cuproptosis-Related Phenotype and Potential Therapeutic Target in Acute Pancreatitis. LID - 10.3390/ijms27209004 [doi] LID - 9004 AB - Acute pancreatitis (AP) is an acute inflammatory disorder of the exocrine pancreas with limited therapies. Whether cuproptosis, a copper-dependent cell death pathway, contributes to AP pathogenesis remains unknown. We integrated single-cell transcriptomics (GSE279876), single-cell transcriptomics (GSE188819), and human whole-blood transcriptomic data (GSE194331) to evaluate 13 cuproptosis-related genes across cell types, along with pseudotime trajectory, regulatory networks, and cell-cell communication. Findings were validated in caerulein-induced severe acute pancreatitis (SAP) mouse models and acinar cell injury using histopathology, electron microscopy, flow cytometry, immunofluorescence, and biochemical assays. Single-cell analysis showed selective activation of a cuproptosis-related signature in acinar cells, with all 13 genes upregulated, most notably GIs and Fdx1. The cuproptosis-related gene signature score increased along with pseudotime, and exploratory transcription factor (TF) and cell-cell communication analyses suggested enrichment of SPI1 and AP-1 (FOS/JUN), alongside MIF/CCL inflammatory signals, which was corroborated in independent datasets. In vitro and in vivo, pancreatic tissues and pancreatic acinar cells in SAP exhibited copper accumulation, FDX1/DLAT upregulation, mitochondrial shrinkage, and cristae loss, which are consistent with a cuproptosis-related phenotype, and were all attenuated by Tetrathiomolybdate (TTM). These findings suggest that the inflammatory microenvironment may be associated with selective activation of a cuproptosis-related program in acinar cells in AP, and that copper chelation with TTM attenuates pancreatic injury, indicating a potential therapeutic strategy targeting copper dyshomeostasis. FAU - Xie, Qingfeng AU - Xie Q AD - The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, China. FAU - He, Maoqin AU - He M AD - The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, China. FAU - Wang, Qi AU - Wang Q AD - Institute of Animal Nutrition and Feed Sciences, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. FAU - Li, Weiqin AU - Li W AUID- ORCID: 0000-0002-8483-6264 AD - The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, China. LA - eng GR - Grant No. BK20251677/Jiangsu Education Department/ PT - Journal Article DEP - 20261011 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 789U1901C5 (Copper) RN - 888Y08971B (Ceruletide) SB - IM MH - *Cuproptosis/genetics MH - Animals MH - Humans MH - *Pancreatitis/genetics/pathology/metabolism/drug therapy MH - Mice MH - Gene Expression Profiling MH - *Copper/metabolism MH - *Single-Cell Analysis/methods MH - *Transcriptome MH - Ceruletide MH - Acinar Cells/metabolism/pathology MH - Single-Cell Gene Expression Analysis MH - Pancreas/metabolism/pathology MH - Phenotype MH - Disease Models, Animal PMC - PMC13705439 OTO - NOTNLM OT - acute pancreatitis OT - cuproptosis OT - inflammatory microenvironment OT - single-cell transcriptomics OT - tetrathiomolybdate COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/11 CRDT- 2026/10/27 10:40 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - ijms27209004 [pii] AID - ijms-27-09004 [pii] AID - 10.3390/ijms27209004 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 11;27(20):9004. doi: 10.3390/ijms27209004. PMID- 42941780 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 11 TI - Special Issue "Molecular Progression of Genetics in Breeding of Farm Animals". LID - 10.3390/ijms27209006 [doi] LID - 9006 AB - This Special Issue, "Molecular Progression of Genetics in Breeding of Farm Animals", aims to map current trends at the intersection of molecular genetics/biology and livestock breeding, as well as to illustrate these trends through selected examples of applied molecular approaches [...]. FAU - Novak, Karel AU - Novak K AUID- ORCID: 0000-0002-9366-3764 AD - Department of Genetics, Genomics and Livestock Breeding, Institute of Animal Science, Pratelstvi 815/109, 104 00 Prague, Czech Republic. LA - eng PT - Editorial PT - Introductory Journal Article DEP - 20261011 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - Animals MH - *Breeding/methods MH - *Animals, Domestic/genetics PMC - PMC13705186 COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/11 CRDT- 2026/10/27 10:40 PHST- 2026/08/14 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - ijms27209006 [pii] AID - ijms-27-09006 [pii] AID - 10.3390/ijms27209006 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 11;27(20):9006. doi: 10.3390/ijms27209006. PMID- 42941779 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 11 TI - Selective Lithium-Magnesium Separation from Brines by Ionic-Liquid Solvent Extraction: Molecular Mechanisms, Extractant Design, and Process Optimization. LID - 10.3390/ijms27209005 [doi] LID - 9005 AB - The growing global demand for lithium (Li(+)), driven by its strategic role in energy storage technologies, faces a critical challenge in its recovery from brines: the selective separation of Li(+) and magnesium (Mg(2+)) ions. Because Li(+) (ionic radius approximately 76 pm) and Mg(2+) ( approximately 72 pm) have almost identical ionic radii despite their different charge, their coordination and hydration behaviour are difficult to discriminate at the molecular level. The high Mg(2+) content in natural brines, such as those from the Atacama Salar, delays the efficiency of conventional extraction processes, which also result in significant Li(+) losses. In this context ionic liquids (ILs) are emerging as alternative solvents with tunable properties, low volatility, and high thermal stability, although their environmental performance depends strongly on their chemical structure, toxicity, aqueous solubility, and recyclability, offering advantages over traditional organic extractants. This study evaluates the use of ionic liquids in liquid-liquid extraction systems for the selective removal of Mg(2+), analysing operational parameters such as pH, organic-to-aqueous phase ratio (O/A), and extractant concentration. This review critically compares ionic-liquid-based extraction systems by relating Mg(2+)/Li(+) selectivity to extractant structure, coordination chemistry, and key operational variables, while also considering the main limitations associated with their application to high-salinity brines. By linking macroscopic extraction performance to the underlying coordination chemistry, hydration-shell disruption, and computational screening tools such as quantum chemical calculations and COSMO-RS, this approach supports the rational, molecular-level design of environmentally responsible extractants for brines with a high Mg/Li ratio. FAU - Mura, Mauricio AU - Mura M AUID- ORCID: 0009-0005-2562-6784 AD - Departamento de Ingenieria Quimica y Procesos de Minerales, Facultad de Ingenieria, Universidad de Antofagasta, Antofagasta 1240000, Chile. FAU - Castillo, Jonathan AU - Castillo J AUID- ORCID: 0000-0002-7794-4291 AD - Departamento de Ingenieria en Metalurgia, Universidad de Atacama, Copiapo 1531772, Chile. FAU - Galvez, Edelmira AU - Galvez E AD - Departamento de Ingenieria Metalurgica y Minas, Universidad Catolica del Norte, Antofagasta 1270709, Chile. FAU - Leiva, Williams AU - Leiva W AUID- ORCID: 0000-0002-3855-9489 AD - Facultad de Ingenieria, Universidad San Sebastian, Sede Concepcion, Concepcion 4030000, Chile. FAU - Hernandez, Pia AU - Hernandez P AUID- ORCID: 0000-0001-9939-8386 AD - Departamento de Ingenieria Quimica y Procesos de Minerales, Facultad de Ingenieria, Universidad de Antofagasta, Antofagasta 1240000, Chile. FAU - Galleguillos-Madrid, Felipe M AU - Galleguillos-Madrid FM AUID- ORCID: 0000-0002-1347-3726 AD - Centro de Desarrollo Energetico Antofagasta, Universidad de Antofagasta, Antofagasta 1240000, Chile. FAU - Sepulveda, Rossana AU - Sepulveda R AUID- ORCID: 0000-0003-0288-8130 AD - Departamento de Ingenieria en Metalurgia, Universidad de Atacama, Copiapo 1531772, Chile. FAU - Toro, Norman AU - Toro N AUID- ORCID: 0000-0003-4273-3563 AD - Faculty of Engineering and Architecture, Universidad Arturo Prat, Avenida Arturo Prat 2120, Iquique 1110939, Chile. LA - eng GR - ATE240006/Agencia Nacional de Investigacion y Desarrollo/ PT - Journal Article PT - Review DEP - 20261011 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 9FN79X2M3F (Lithium) RN - 0 (Ionic Liquids) RN - I38ZP9992A (Magnesium) RN - 0 (Solvents) RN - 0 (Salts) RN - 0 (brine) SB - IM MH - *Lithium/chemistry/isolation & purification MH - *Ionic Liquids/chemistry MH - *Magnesium/chemistry/isolation & purification MH - *Liquid-Liquid Extraction/methods MH - Solvents/chemistry MH - Salts/chemistry PMC - PMC13704900 OTO - NOTNLM OT - alternative solvents OT - direct lithium extraction (DLE) OT - high Mg/Li ratio OT - ion coordination chemistry OT - liquid-liquid extraction OT - lithium purification OT - magnesium removal OT - quantum chemical calculations COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/11 CRDT- 2026/10/27 10:40 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - ijms27209005 [pii] AID - ijms-27-09005 [pii] AID - 10.3390/ijms27209005 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 11;27(20):9005. doi: 10.3390/ijms27209005. PMID- 42941778 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 11 TI - Cellular Senescence in Ocular Surface Diseases: Molecular Mechanisms and Therapeutic Perspectives. LID - 10.3390/ijms27209003 [doi] LID - 9003 AB - Cellular senescence is characterized by stable cell-cycle arrest and a senescence-associated secretory phenotype (SASP). Recurrent desiccating, oxidative, ultraviolet, microbial, and immune-mediated insults may induce stress-induced premature senescence (SIPS) in ocular surface tissues. PubMed and Web of Science were searched from database inception to 30 June 2026. Primary human, animal, and in vitro studies were prioritized, and direct evidence of senescence was distinguished from indirect stress-related or SASP-like associations. The p53-p21(CIP1)-pRB and p16(INK4a)-pRB pathways mediate senescence-associated cell-cycle arrest. Reactive oxygen species (ROS)-p38 mitogen-activated protein kinase (p38 MAPK) and cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) signaling act as stress-sensing and inflammatory pathways, whereas mechanistic target of rapamycin (mTOR) and AMP-activated protein kinase (AMPK) contribute to SASP and metabolic regulation. Transient senescence may support wound repair and immune clearance, whereas persistent senescence may contribute to epithelial and endothelial dysfunction, tear-film instability, immune dysregulation, fibrosis, and extracellular matrix remodeling. Mechanistic support is strongest in selected experimental models; evidence in several human ocular surface disorders remains associative. Cellular senescence is a context-dependent contributor to ocular surface pathology. Senolytic and senomorphic strategies remain predominantly preclinical and require disease-, cell-, and stage-specific validation. FAU - Zhang, Linkang AU - Zhang L AD - Eye Hospital, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China. FAU - Li, Shipeng AU - Li S AD - Department of Ophthalmology, The Second Affiliated Hospital of Harbin Medical University, Harbin 150001, China. FAU - Gao, Qiao AU - Gao Q AD - Eye Hospital, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China. FAU - Zhang, Hongkun AU - Zhang H AD - Second Affiliated Hospital of Heilongjiang TCM University, Harbin 150001, China. FAU - Zhang, Hong AU - Zhang H AUID- ORCID: 0000-0003-4179-5666 AD - Eye Hospital, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China. FAU - Wang, Shudan AU - Wang S AUID- ORCID: 0000-0002-9292-0992 AD - Eye Hospital, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China. LA - eng GR - 82301164/National Natural Science Foundation of China/ GR - 2024YQ05/Outstanding Young Scholar Fund of the First Affiliated Hospital of Harbin Medical University/ PT - Journal Article PT - Review DEP - 20261011 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Reactive Oxygen Species) SB - IM MH - Humans MH - *Cellular Senescence MH - Animals MH - *Senescence-Associated Secretory Phenotype MH - *Eye Diseases/metabolism/pathology/therapy MH - Signal Transduction MH - Reactive Oxygen Species/metabolism PMC - PMC13704741 OTO - NOTNLM OT - cellular senescence OT - ocular surface disease OT - senescence-associated secretory phenotype OT - senolytics OT - senomorphics OT - stress-induced premature senescence COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/11 CRDT- 2026/10/27 10:40 PHST- 2026/08/23 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - ijms27209003 [pii] AID - ijms-27-09003 [pii] AID - 10.3390/ijms27209003 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 11;27(20):9003. doi: 10.3390/ijms27209003. PMID- 42941777 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 11 TI - Distinct Clinical Patterns Associated with HLA-DRB1 Genotypes in Pediatric Autoimmune Liver Diseases. LID - 10.3390/ijms27209002 [doi] LID - 9002 AB - Autoimmune liver diseases (AILD), including autoimmune hepatitis (AIH) and autoimmune sclerosing cholangitis (ASC), are rare but important causes of chronic liver disease in children. Although HLA-DRB1 alleles have been associated with disease susceptibility, their role in modulating the clinical phenotype and long-term outcome of pediatric AILDs remains incompletely understood. We aimed to characterize the distribution of HLA-DRB1 alleles and genotype combinations and describe the accompanying clinical features in a pediatric AILD cohort. Thirty children diagnosed with AIH-1 (n = 14), AIH-2 (n = 6), or ASC (n = 10) underwent HLA-DRB1 genotyping. Clinical presentation, fibrosis stage, treatment response, and associated autoimmune diseases were retrospectively analyzed. The most frequent alleles copies were HLA-DRB1*03 (n = 15), predominantly identified in AIH, and HLA-DRB1*13 (n = 14), identified in both AIH and ASC. Genotype combinations involving these alleles were observed across a broad spectrum of clinical presentations, fibrosis stages, treatment responses, and extrahepatic autoimmune manifestations. In this small cohort, HLA-DRB1*14-containing genotypes were observed in several patients with severe disease features, including advanced fibrosis, cirrhosis, treatment-refractory disease, and ulcerative colitis. Patients carrying HLA-DRB1*11, HLA-DRB1*15, or HLA-DRB1*16 had favorable hepatic outcomes despite severe initial presentation or associated extrahepatic autoimmune manifestations; however, the number of carriers was small. Clinical features varied among patients carrying different genotype combinations; however, the independent contribution of the accompanying allele could not be assessed. Our findings illustrate marked clinical heterogeneity among children carrying different HLA-DRB1 alleles and genotype combinations. In this exploratory cohort, selected HLA-DRB1 combinations co-occurred with distinct clinical patterns. HLA profiling may have a role in future risk stratification and individualized follow-up of children with AILD, but these observations are hypothesis-generating, they do not support genotype-based clinical management and require validation in larger, multicenter cohorts. FAU - Grama, Alina AU - Grama A AD - 2nd Pediatric Discipline, Department of Mother and Child, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. AD - 2nd Pediatric Clinic, Center of Expertise in Pediatric Liver Rare Diseases, Emergency Clinical Hospital for Children, 400177 Cluj-Napoca, Romania. FAU - Mititelu, Alexandra AU - Mititelu A AUID- ORCID: 0000-0002-0955-4486 AD - 2nd Pediatric Discipline, Department of Mother and Child, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. AD - 2nd Pediatric Clinic, Center of Expertise in Pediatric Liver Rare Diseases, Emergency Clinical Hospital for Children, 400177 Cluj-Napoca, Romania. FAU - Benta, Gabriel AU - Benta G AUID- ORCID: 0000-0002-2099-8379 AD - 2nd Pediatric Discipline, Department of Mother and Child, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. AD - 2nd Pediatric Clinic, Center of Expertise in Pediatric Liver Rare Diseases, Emergency Clinical Hospital for Children, 400177 Cluj-Napoca, Romania. FAU - Sucaciu, Raisa AU - Sucaciu R AD - 2nd Pediatric Discipline, Department of Mother and Child, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. FAU - Popescu, Daniel AU - Popescu D AD - 2nd Pediatric Discipline, Department of Mother and Child, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. FAU - Maris, Bianca AU - Maris B AD - 2nd Pediatric Discipline, Department of Mother and Child, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. FAU - Onaciu, Alexia AU - Onaciu A AD - 2nd Pediatric Discipline, Department of Mother and Child, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. FAU - Agoston, Stefan AU - Agoston S AD - 2nd Pediatric Discipline, Department of Mother and Child, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. FAU - Pop, Tudor Lucian AU - Pop TL AUID- ORCID: 0000-0002-4931-1219 AD - 2nd Pediatric Discipline, Department of Mother and Child, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. AD - 2nd Pediatric Clinic, Center of Expertise in Pediatric Liver Rare Diseases, Emergency Clinical Hospital for Children, 400177 Cluj-Napoca, Romania. LA - eng GR - No. 32154/12/16.12.2024/Iuliu Hatieganu University of Medicine and Pharmacy/ PT - Journal Article DEP - 20261011 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (HLA-DRB1 Chains) SB - IM MH - Humans MH - *HLA-DRB1 Chains/genetics MH - Female MH - Child MH - Male MH - *Hepatitis, Autoimmune/genetics/pathology MH - Genotype MH - *Cholangitis, Sclerosing/genetics/pathology MH - Alleles MH - Child, Preschool MH - Genetic Predisposition to Disease MH - Adolescent MH - Retrospective Studies MH - *Autoimmune Diseases/genetics MH - Gene Frequency MH - Infant MH - *Liver Diseases/genetics PMC - PMC13704733 OTO - NOTNLM OT - HLA-DRB1 OT - autoimmune hepatitis OT - autoimmune sclerosing cholangitis OT - genotype-phenotype correlation OT - liver fibrosis OT - pediatric autoimmune liver disease COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/11 CRDT- 2026/10/27 10:40 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - ijms27209002 [pii] AID - ijms-27-09002 [pii] AID - 10.3390/ijms27209002 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 11;27(20):9002. doi: 10.3390/ijms27209002. PMID- 42941772 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 11 TI - Field Density Variation and Metabolite Profiling Reveal Transcriptome Mechanisms Driving Salt-Responsive Root Flavone Accumulation in Scutellaria baicalensis. LID - 10.3390/ijms27208996 [doi] LID - 8996 AB - Root flavones, especially baicalin, baicalein, wogonoside, and wogonin, contribute substantially to the medicinal quality of Scutellaria baicalensis Georgi. In the arid and semi-arid regions where this crop is grown, salinized soils constrain establishment but reshape specialized metabolism. We used field density trials, salt-stress experiments, ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), and time-series RNA-seq, followed by weighted gene co-expression network analysis (WGCNA), to ask whether agronomic practices and salt-induced transcriptional changes alter root flavone content. Planting density showed a clear yield-quality trade-off: higher density increased belowground biomass per unit area but reduced individual root growth and flavone content, with 150 plants.m(-2) providing a practical balance between population yield and root quality under the tested Xinjiang field conditions. In established seedlings, 0.5% NaCl caused no visible damage over 24 h and specifically raised baicalin and baicalein at 12 h, whereas wogonoside and wogonin (the wogonin-type flavones) remained nearly unchanged. Time-series RNA-seq identified thousands of differentially expressed genes between 2 and 24 h. WGCNA identified a turquoise module (one of the color-named co-expression modules) that was enriched for genes involved in carbohydrate metabolism and cytoskeletal/microtubule processes. Salt-responsive NAC, MYB and WRKY transcription factors co-expressed with core structural genes (PAL, 4CL, CHS, CHI); one candidate, SbNAC86, was up-regulated mainly at 24 h after the metabolite peak, consistent with a potential role in the later phase of the response rather than in triggering the initial accumulation peak. These findings outline the temporal dynamics of salt-induced branch-selective flavone accumulation and suggest candidate regulators for functional validation, with implications for quality-focused cultivation on saline marginal lands. FAU - Lin, Zirun AU - Lin Z AUID- ORCID: 0009-0001-0147-5046 AD - Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, College of Life Sciences, Shihezi University, Shihezi 832003, China. FAU - Zhang, Yaxin AU - Zhang Y AD - Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, College of Life Sciences, Shihezi University, Shihezi 832003, China. FAU - Zhao, Chenjie AU - Zhao C AD - Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, College of Life Sciences, Shihezi University, Shihezi 832003, China. FAU - Zhao, Kun AU - Zhao K AD - Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, College of Life Sciences, Shihezi University, Shihezi 832003, China. FAU - Ma, Denglin AU - Ma D AD - Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, College of Life Sciences, Shihezi University, Shihezi 832003, China. FAU - Zhang, Tao AU - Zhang T AD - Pharmaceutical Research Institute, Urumqi 830010, China. FAU - Wang, Manhong AU - Wang M AD - Department of Poultry Science, Mississippi State University, Starkville, MS 39762, USA. FAU - Yao, Hua AU - Yao H AD - Key Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, College of Life Sciences, Shihezi University, Shihezi 832003, China. LA - eng GR - 32260083/National Natural Science Foundation of China/ GR - 82560747/National Natural Science Foundation of China/ GR - RCZK202595/the Shihezi University High-level Talent Research Startup Project/ GR - 2026YD025/the Central Fund for Local Science and Technology Development/ GR - 2025CC007/the Science and Technology Special Project/ GR - KY2025JBGS03/the Financial Science and Technology Plan of Tumushuke City/ GR - 2024ZDJS139/the Key Field Projects of the Guangdong Province Key Construction Discipline Research Capacity Enhancement Project/ GR - 2026NY05/the Key Field Science and Technology Research Project under the Financial Science and Technology Plan of Shihezi City/ PT - Journal Article DEP - 20261011 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Flavones) RN - 0 (Flavonoids) RN - 0 (Flavanones) RN - POK93PO28W (wogonin) RN - ETX4944Z3R (wogonoside) RN - 451W47IQ8X (Sodium Chloride) RN - 49QAH60606 (baicalein) RN - 0 (Glucosides) SB - IM MH - *Scutellaria baicalensis/genetics/metabolism/growth & development MH - *Plant Roots/metabolism/genetics MH - Gene Expression Regulation, Plant MH - *Transcriptome MH - *Flavones/metabolism MH - Flavonoids/metabolism MH - Flavanones/metabolism MH - Salt Stress MH - Sodium Chloride/pharmacology MH - Gene Expression Profiling MH - *Metabolome MH - Glucosides/metabolism PMC - PMC13704419 OTO - NOTNLM OT - 4'-deoxyflavones OT - NAC transcription factors OT - Scutellaria baicalensis OT - WGCNA OT - baicalein OT - baicalin OT - planting density OT - salt stress OT - transcriptome OT - wogonin OT - wogonoside COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/11 CRDT- 2026/10/27 10:40 PHST- 2026/09/22 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - ijms27208996 [pii] AID - ijms-27-08996 [pii] AID - 10.3390/ijms27208996 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 11;27(20):8996. doi: 10.3390/ijms27208996. PMID- 42941768 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 10 TI - Diagnostic and Predictive CSF Biomarkers in Idiopathic Normal Pressure Hydrocephalus (iNPH): A Systematic Review. LID - 10.3390/ijms27208986 [doi] LID - 8986 AB - Idiopathic normal pressure hydrocephalus (iNPH) is a progressive disorder clinically characterized by Hakim's triad: gait disturbance, cognitive impairment, and urinary incontinence. iNPH treatment consists of neurosurgical implantation of a ventriculoperitoneal CSF diversion, whose outcome depends on an early and correct diagnosis. Currently, there is no specific diagnostic test to perform preoperatively. Still, the combination of neurological examination, gait analysis, neurocognitive evaluation, and the tap test is useful to suggest when surgery is indicated. Our systematic review aims to identify diagnostic and outcome predictor CSF biomarkers (BM) in iNPH. The present systematic review was conducted following PRISMA 2020 guidelines. PubMed, Scopus, and the Cochrane Library databases were first consulted in April 2025 and updated in May 2026. Studies were selected using the terms "idiopathic normal pressure hydrocephalus" and "CSF biomarkers" under Medical Subject Headings (MeSH) as inclusion criteria. The eligibility of a record was defined according to the study title and abstract. The QUADAS-2, QUIPS, ROBINS-I V2, and RobVis tools were used to assess the risk of bias. Ninety-nine records were included in this systematic review. Amyloid proteins (Abeta40-42), followed by tau (tauT, tauP-181) and neuroinflammatory proteins were the most frequently CSF BM analyzed in patients with iNPH. ELISA was the most used method to measure BM in CSF samples. Forty-seven records investigated the potential diagnostic role of CSF BM; comparisons were frequently made among iNPH, Alzheimer's disease (AD), and healthy control CSF samples. Regarding outcome prediction, 28 records examined CSF BM, with heterogeneous comparisons such as lumbar versus ventricular CSF or shunted versus non-shunted iNPH CSF samples, including healthy controls and iNPH. Seven records proposed both the diagnostic and predictive role of some CSF biomarkers, especially for tau proteins and Abeta42. On the other hand, 17 records concluded that neither a diagnostic nor a predictive role could be proposed for the CSF BM they analyzed. However, these records increased knowledge about an iNPH CSF pattern. Collecting and analyzing CSF samples from iNPH patients can be advantageous in differential diagnosis with other similar neurodegenerative disorders, especially AD, and therefore a predictor of neurosurgical outcomes. Combined biomarker panels should be integrated in standardized protocols, measuring: Abeta42, Abeta42/Abeta40, p-tau, total tau, ADAbeta42/p-tau, L-PGDS, NFL and NRGN. FAU - Procopio, Emanuela AU - Procopio E AUID- ORCID: 0009-0000-2650-1581 AD - Division of Neurosurgery, Department of Biomedical, Dental and Morphological and Functional Imaging, University of Messina, Via Consolare Valeria, 98125 Messina, Italy. FAU - Accattatis, Arianna AU - Accattatis A AD - Division of Neurosurgery, Department of Biomedical, Dental and Morphological and Functional Imaging, University of Messina, Via Consolare Valeria, 98125 Messina, Italy. FAU - Stroscio, Carmelino Angelo AU - Stroscio CA AD - Division of Neurosurgery, Department of Medical and Surgical Sciences, University Magna Graecia of Catanzaro, 88100 Catanzaro, Italy. FAU - Guzzi, Giusy AU - Guzzi G AD - Division of Neurosurgery, Department of Medical and Surgical Sciences, University Magna Graecia of Catanzaro, 88100 Catanzaro, Italy. FAU - Lavano, Angelo AU - Lavano A AUID- ORCID: 0000-0001-8180-8391 AD - Division of Neurosurgery, Department of Medical and Surgical Sciences, University Magna Graecia of Catanzaro, 88100 Catanzaro, Italy. FAU - La Torre, Domenico AU - La Torre D AUID- ORCID: 0000-0002-3918-8798 AD - Neurosurgical Unit, Department of Pharmacy, Health and Nutrition Sciences, University of Calabria, 87036 Rende, Italy. FAU - Della Torre, Attilio AU - Della Torre A AUID- ORCID: 0000-0003-0872-0152 AD - Division of Neurosurgery, Department of Medical and Surgical Sciences, University Magna Graecia of Catanzaro, 88100 Catanzaro, Italy. LA - eng PT - Journal Article PT - Review PT - Systematic Review DEP - 20261010 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Biomarkers) RN - 0 (Amyloid beta-Peptides) RN - 0 (tau Proteins) RN - 0 (Peptide Fragments) RN - 0 (amyloid beta-protein (1-42)) SB - IM MH - *Hydrocephalus, Normal Pressure/cerebrospinal fluid/diagnosis MH - Humans MH - *Biomarkers/cerebrospinal fluid MH - Amyloid beta-Peptides/cerebrospinal fluid MH - tau Proteins/cerebrospinal fluid MH - Peptide Fragments/cerebrospinal fluid PMC - PMC13704644 OTO - NOTNLM OT - CSF biomarkers in iNPH OT - CSF lipidomics and proteomics OT - NPH differential diagnosis OT - VP shunt outcome prediction OT - amyloid and Tau dynamics in CSF OT - glymphatic clearance in hydrocephalus OT - idiopathic Normal Pressure Hydrocephalus (iNPH) OT - neuroinflammation in iNPH COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/10 CRDT- 2026/10/27 10:40 PHST- 2026/07/23 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - ijms27208986 [pii] AID - ijms-27-08986 [pii] AID - 10.3390/ijms27208986 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 10;27(20):8986. doi: 10.3390/ijms27208986. PMID- 42941758 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 10 TI - Urinary Metabolomics in Bladder Cancer: A Scoping Review of Current Evidence and Translation Implications. LID - 10.3390/ijms27208983 [doi] LID - 8983 AB - Bladder cancer (BC) diagnosis and surveillance currently rely largely on invasive cystoscopy, making urine an attractive non-invasive biofluid for discovering metabolic biomarkers, given its direct contact with the bladder urothelium. This review provides a comprehensive and critical synthesis of the literature on urinary metabolomic profiling in BC, summarising metabolites and metabolite panels associated with the disease, their relevant metabolic pathways, and diagnostic performance. A search was conducted in PubMed, complemented by records from reference lists of relevant reviews. After screening, 30 original studies comparing urinary metabolomic profiles between BC patients and cancer-free controls were included, encompassing diverse analytical platforms and statistical approaches. A wide range of urinary metabolites were reported to be altered between BC patients and controls, of which 52 were recurrently identified in at least two independent studies. Increased levels of taurine, valine, lactate, adenosine monophosphate and adipate were among the most consistent metabolic alterations, whereas the levels of citrate, glycerol, creatinine, and several dicarboxylic acids and acylcarnitines were consistently decreased. Other metabolites, such as hippurate, tyrosine, and histidine, showed variable trends in their levels. These alterations may reflect metabolic processes associated with tumour growth, energy reprogramming, lipid metabolism, and oxidative stress adaptation. Most studies also proposed multi-metabolite panels combining metabolites to discriminate BC patients from controls, achieving sensitivity and specificity up to 100% and AUC up to 0.983, while fewer studies explored panels for tumour stage and grade discrimination, with more modest performance. Overall, urinary metabolomics reveals a broad, biologically coherent set of metabolic alterations associated with BC, but methodological heterogeneity and limited independent validation still hinder clinical translation. Larger, harmonised, prospectively validated studies integrating multi-omics and machine learning are needed to establish robust urinary biomarkers for BC. FAU - Carapito, Angela AU - Carapito A AUID- ORCID: 0000-0003-1396-4485 AD - Associate Laboratory i4HB-Institute for Health and Bioeconomy, University of Porto, 4050-313 Porto, Portugal. AD - UCIBIO-Applied Molecular Biosciences Unit, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal. FAU - Amaro, Filipa AU - Amaro F AD - Associate Laboratory i4HB-Institute for Health and Bioeconomy, University of Porto, 4050-313 Porto, Portugal. AD - UCIBIO-Applied Molecular Biosciences Unit, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal. FAU - Roque, Ana Cecilia AU - Roque AC AUID- ORCID: 0000-0002-4586-3024 AD - Associate Laboratory i4HB-Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University of Lisbon, 2829-516 Caparica, Portugal. AD - UCIBIO-Applied Molecular Biosciences Unit, Department of Chemistry, NOVA School of Science and Technology, NOVA University of Lisbon, 2829-516 Caparica, Portugal. FAU - Carvalho, Felix AU - Carvalho F AUID- ORCID: 0000-0003-3858-3494 AD - Associate Laboratory i4HB-Institute for Health and Bioeconomy, University of Porto, 4050-313 Porto, Portugal. AD - UCIBIO-Applied Molecular Biosciences Unit, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal. FAU - Pinto, Joana AU - Pinto J AUID- ORCID: 0000-0001-9719-8364 AD - Associate Laboratory i4HB-Institute for Health and Bioeconomy, University of Porto, 4050-313 Porto, Portugal. AD - UCIBIO-Applied Molecular Biosciences Unit, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal. FAU - Guedes de Pinho, Paula AU - Guedes de Pinho P AUID- ORCID: 0000-0002-7395-5700 AD - Associate Laboratory i4HB-Institute for Health and Bioeconomy, University of Porto, 4050-313 Porto, Portugal. AD - UCIBIO-Applied Molecular Biosciences Unit, Laboratory of Toxicology, Faculty of Pharmacy, University of Porto, 4050-313 Porto, Portugal. LA - eng GR - UID/04378/2025/Fundacao para a Ciencia e Tecnologia/ GR - UID/PRR/04378/2025/Fundacao para a Ciencia e Tecnologia/ PT - Journal Article PT - Scoping Review DEP - 20261010 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Biomarkers, Tumor) SB - IM MH - Humans MH - *Biomarkers, Tumor/urine/metabolism MH - *Metabolome MH - *Metabolomics/methods MH - *Urinary Bladder Neoplasms/urine/metabolism/diagnosis PMC - PMC13704385 OTO - NOTNLM OT - biomarker discovery OT - bladder cancer OT - metabolic profiling OT - tumour stage and grade OT - urinary metabolomics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/10 CRDT- 2026/10/27 10:40 PHST- 2026/09/22 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - ijms27208983 [pii] AID - ijms-27-08983 [pii] AID - 10.3390/ijms27208983 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 10;27(20):8983. doi: 10.3390/ijms27208983. PMID- 42941752 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 10 TI - Genome-Informed Loop-Mediated Isothermal Amplification (LAMP) Assays for Detection and Differentiation of Ralstonia pseudosolanacearum Phylotypes I and III. LID - 10.3390/ijms27208976 [doi] LID - 8976 AB - The Ralstonia solanacearum species complex (RSSC) causes bacterial wilt in a broad range of economically important hosts. The RSSC has been reclassified into three species, of which R. solanacearum sequevars 1 and 2 in phylotype IIB are designated as Select Agents in the U.S. Many rapid tests for "R. solanacearum" target the RSSC and thus cross-react with R. pseudosolanacearum, which is endemic to the U.S. To avoid false positive identifications and unnecessary triggering of Select Agent measures, four portable LAMP-based screening assay variations were developed and validated for specific detection of R. pseudosolanacearum: real-time LAMP assays for detection of R. pseudosolanacearum (1) and differentiation of R. pseudosolanacearum phylotypes I and III in the AmpliFire((R)) Isothermal Fluorometer (2) or Genie((R)) III (3), and a colorimetric LAMP for detection of R. pseudosolanacearum (4). All assays demonstrated high sensitivity (10-50 colony-forming units (CFU)/reaction) and specificity; 100% analytical specificity and selectivity based on 177 RSSC isolates (81 phylotype I, 70 phylotype II, 15 phylotype III and 11 phylotype IV) and 53 non-RSSC bacterial pathogens on relevant hosts and soil, and 100% diagnostic specificity and sensitivity based on testing inoculated plants and a Euphresco sample panel. The assays presented here represent useful diagnostic tools that can be deployed on-site to aid domestic survey and detection efforts. FAU - Karakkat, Brijesh B AU - Karakkat BB AD - Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC 27607, USA. AD - Plant Pathogen Confirmatory Diagnostics Laboratory (PPCDL), Science and Technology (S&T), Plant Protection and Quarantine (PPQ), Animal and Plant Health Inspection Service (APHIS), United States Department of Agriculture (USDA), Laurel, MD 20708, USA. FAU - Huang, Qi AU - Huang Q AD - Floral and Nursery Plants Research Unit, United States National Arboretum, USDA Agricultural Research Service (ARS), Beltsville, MD 20705, USA. FAU - Nunziata, Schyler O AU - Nunziata SO AD - Plant Pathogen Confirmatory Diagnostics Laboratory (PPCDL), Science and Technology (S&T), Plant Protection and Quarantine (PPQ), Animal and Plant Health Inspection Service (APHIS), United States Department of Agriculture (USDA), Laurel, MD 20708, USA. FAU - Yasuhara-Bell, Jarred AU - Yasuhara-Bell J AUID- ORCID: 0000-0001-5365-8170 AD - Plant Pathogen Confirmatory Diagnostics Laboratory (PPCDL), Science and Technology (S&T), Plant Protection and Quarantine (PPQ), Animal and Plant Health Inspection Service (APHIS), United States Department of Agriculture (USDA), Laurel, MD 20708, USA. LA - eng PT - Journal Article DEP - 20261010 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (DNA, Bacterial) RN - LAMP assay RN - Ralstonia pseudosolanacearum SB - IM MH - *Nucleic Acid Amplification Techniques/methods MH - *Ralstonia/genetics/isolation & purification/classification MH - *Genome, Bacterial MH - Sensitivity and Specificity MH - Phylogeny MH - *Molecular Diagnostic Techniques/methods MH - Ralstonia solanacearum/genetics MH - Plant Diseases/microbiology MH - DNA, Bacterial/genetics PMC - PMC13704481 OTO - NOTNLM OT - LAMP OT - Ralstonia pseudosolanacearum OT - bacterial wilt OT - detection OT - differentiation OT - loop-mediated amplification OT - phylotype I OT - phylotype III COIS- The authors declare no conflicts of interest. The findings and conclusions in this publication are those of the authors and should not be construed to represent any official USDA or U.S. Government determination or policy. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. Equal Opportunity/Non-Discrimination Statement: In accordance with Federal civil rights law and U.S. Department of Agriculture (USDA) civil rights regulations and policies, the USDA, its Agencies, offices, and employees, and institutions participating in or administering USDA programs are prohibited from discriminating based on race, color, national origin, religion, sex, disability, age, marital status, family/parental status, income derived from a public assistance program, political beliefs, or reprisal or retaliation for prior civil rights activity, in any program or activity conducted or funded by USDA (not all bases apply to all programs). Remedies and complaint filing deadlines vary by program or incident. Persons with disabilities who require alternative means of communication for program information (e.g., Braille, large print, audiotape, American Sign Language, etc.) should contact the State or local Agency that administers the program or contact USDA through the Telecommunications Relay Service at 711 (voice and TTY). Additionally, program information may be made available in languages other than English. To file a program discrimination complaint, complete the USDA Program Discrimination Complaint Form, AD-3027, found online at How to File a Program Discrimination Complaint (https://www.usda.gov/about-usda/general-information/staff-offices/office-assistant-secretary-civil-rights/how-file-usda-discrimination-complaint/how-file-program-discrimination-complaint) and at any USDA office, or write a letter addressed to USDA and provide in the letter all of the information requested in the form. To request a copy of the complaint form, call (866) 632-9992. Submit your completed form or letter to USDA by: (1) mail: U.S. Department of Agriculture, Office of the Assistant Secretary for Civil Rights, 1400 Independence Avenue, SW, Mail Stop 9410, Washington, D.C. 20250-9410; (2) fax: (202) 690-7442; or (3) email: program.intake@usda.gov. USDA is an equal opportunity provider, employer, and lender. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/10 CRDT- 2026/10/27 10:40 PHST- 2026/09/18 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - ijms27208976 [pii] AID - ijms-27-08976 [pii] AID - 10.3390/ijms27208976 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 10;27(20):8976. doi: 10.3390/ijms27208976. PMID- 42941750 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 10 TI - Targeting EGR1 Attenuates CCL2 Expression in Inflammatory Keratinocytes and Allergic Skin Inflammation. LID - 10.3390/ijms27208973 [doi] LID - 8973 AB - Early growth response 1 (EGR1) is a zinc-finger transcription factor involved in inflammatory gene regulation, but the extent to which pharmacological inhibition of EGR1 DNA-binding activity influences inflammatory transcriptional responses in keratinocytes remains incompletely understood. Here, we explored the transcriptional effects of pharmacological EGR1 inhibition in tumor necrosis factor-alpha (TNFalpha)-stimulated HaCaT keratinocytes. RNA sequencing was employed as an exploratory, hypothesis-generating screen using three structurally related EGR1 inhibitors (IT21, IT23, and IT25) to identify candidate genes showing concordant expression patterns across the three pharmacological perturbations. This exploratory analysis suggested broader changes in inflammation-associated transcriptional programs and identified several leukocyte-recruiting chemokines among the candidate genes. CCL2 was selected as a representative candidate for independent biological and mechanistic validation. IT25 treatment and EGR1 knockdown attenuated TNFalpha-induced CCL2 expression, while promoter-reporter analysis, EGR1-binding site mutagenesis, EGR1 overexpression, and chromatin immunoprecipitation supported EGR1-dependent transcriptional regulation of CCL2 in keratinocytes. Furthermore, topical IT25 treatment attenuated house dust mite-induced atopic dermatitis-like skin inflammation and was associated with reduced epidermal CCL2 expression and inflammatory cell infiltration in vivo. Collectively, these findings establish EGR1-dependent regulation of CCL2 in inflammatory keratinocytes and support further investigation of EGR1 DNA-binding activity as a pharmacological target in cutaneous inflammation. FAU - Choi, Yena AU - Choi Y AD - Department of Biological Sciences, College of Life Science, Konkuk University, Seoul 05029, Republic of Korea. FAU - Jung, Euitaek AU - Jung E AUID- ORCID: 0000-0003-3091-0649 AD - Department of Biological Sciences, College of Life Science, Konkuk University, Seoul 05029, Republic of Korea. FAU - Han, Junekyu AU - Han J AUID- ORCID: 0009-0001-2209-0217 AD - Department of Biological Sciences, College of Life Science, Konkuk University, Seoul 05029, Republic of Korea. FAU - Shin, Soon Young AU - Shin SY AUID- ORCID: 0000-0002-1075-0011 AD - Department of Biological Sciences, College of Life Science, Konkuk University, Seoul 05029, Republic of Korea. AD - Cancer and Metabolism Institute, Konkuk University, Seoul 05029, Republic of Korea. LA - eng GR - HN21C0984/Korea Drug Development Fund/ GR - 2023R1A2C1003601/National Research Foundation of Korea/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Early Growth Response Protein 1) RN - 0 (Chemokine CCL2) RN - 0 (Tumor Necrosis Factor-alpha) RN - 0 (EGR1 protein, human) RN - 0 (CCL2 protein, human) SB - IM MH - *Early Growth Response Protein 1/metabolism/genetics/antagonists & inhibitors MH - *Chemokine CCL2/genetics/metabolism MH - *Keratinocytes/metabolism/drug effects/pathology MH - Humans MH - Animals MH - Tumor Necrosis Factor-alpha/pharmacology MH - Mice MH - Gene Expression Regulation/drug effects MH - *Dermatitis, Atopic/metabolism/genetics/pathology/drug therapy MH - HaCaT Cells MH - Inflammation/metabolism/genetics MH - Promoter Regions, Genetic MH - Cell Line PMC - PMC13705188 OTO - NOTNLM OT - CCL2 OT - Early growth response 1 (EGR1) OT - RNA sequencing OT - chemokine network OT - house dust mite OT - keratinocyte OT - skin inflammation OT - transcription factor inhibitor COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/10 CRDT- 2026/10/27 10:40 PHST- 2026/08/20 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - ijms27208973 [pii] AID - ijms-27-08973 [pii] AID - 10.3390/ijms27208973 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 10;27(20):8973. doi: 10.3390/ijms27208973. PMID- 42941748 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 10 TI - From Gene-Based Breeding to Haplotype-Based Breeding for Rice (Oryza sativa) Improvement in the Genomics Era. LID - 10.3390/ijms27208972 [doi] LID - 8972 AB - Rice (Oryza sativa L.) improvement increasingly uses genome-scale variation, but breeding decisions remain trait-oriented and depend on genetic architecture, recipient background, target environment, and selection resources. This review assesses when phased haplotype information adds value beyond causal-gene markers, single-nucleotide polymorphism assays, phenotypic selection, and genomic prediction. Reported configurations for yield, grain quality, nutrition, biotic resistance, and abiotic-stress adaptation are evaluated according to population structure, carrier frequency, effect uncertainty, haplotype-by-background interaction, haplotype-by-environment interaction, linkage drag, transferability, assay availability, and current breeding use. A three-tier hierarchy classifies configurations as Candidate, Validated, or Breeder-Ready. Most reported favorable haplotypes remain Candidate targets, fewer have controlled genetic validation, and few sequence-defined multi-variant haplotypes have documented use in active breeding programs. Practical deployment requires stable sequence nomenclature, accessible donors, diagnostic assays, elite-background and multi-environment evaluation, cost analysis, and donor-segment recovery. Graph pangenomes, phased long-read assemblies, causal reconstruction, and prospective comparisons with current selection methods can improve future assessment. Haplotype information has greatest value when phase improves a defined breeding decision and increases target-environment performance, annual genetic gain, selection efficiency, or cultivar delivery. FAU - Nguyen, Trung Quoc AU - Nguyen TQ AUID- ORCID: 0000-0003-1123-3419 AD - Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi City 100000, Vietnam. FAU - Ho, Anh Quynh AU - Ho AQ AUID- ORCID: 0009-0007-6973-6621 AD - Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi City 100000, Vietnam. FAU - Chu, Ha Duc AU - Chu HD AUID- ORCID: 0000-0003-0087-9994 AD - Faculty of Agricultural Technology, University of Engineering and Technology, Vietnam National University Hanoi, Hanoi City 100000, Vietnam. AD - VinUni Bigdata Research Institute, VinUniversity, Hanoi City 100000, Vietnam. FAU - Chuong, Nguyen Nguyen AU - Chuong NN AUID- ORCID: 0000-0001-9631-9953 AD - Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409, USA. FAU - Anik, Touhidur Rahman AU - Anik TR AUID- ORCID: 0000-0003-1995-494X AD - Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409, USA. FAU - Dong, Gioi Huy AU - Dong GH AUID- ORCID: 0000-0003-4977-3576 AD - Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi City 100000, Vietnam. FAU - Sulieman, Saad AU - Sulieman S AUID- ORCID: 0000-0003-2070-6660 AD - Department of Integrative Agriculture, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain P.O. Box 15551, Abu Dhabi, United Arab Emirates. FAU - Nguyen, Nguyet Thi Minh AU - Nguyen NTM AUID- ORCID: 0009-0003-0978-3996 AD - Agricultural Genetics Institute, Vietnam Academy of Agricultural Sciences, Hanoi City 100000, Vietnam. FAU - Jogaiah, Sudisha AU - Jogaiah S AUID- ORCID: 0000-0003-2831-651X AD - Department of Environmental Science, Central University of Kerala, Kasaragod 671316, India. FAU - La, Hong Viet AU - La HV AUID- ORCID: 0000-0002-6016-5857 AD - Institute of Scientific Research and Applications, Hanoi Pedagogical University 2, Phuc Yen City 35000, Vietnam. FAU - Tran, Lam-Son Phan AU - Tran LP AUID- ORCID: 0000-0001-9883-9768 AD - Institute of Genomics for Crop Abiotic Stress Tolerance, Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409, USA. LA - eng PT - Journal Article PT - Review DEP - 20261010 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - *Oryza/genetics MH - *Haplotypes MH - *Plant Breeding/methods MH - *Genomics/methods MH - Genome, Plant MH - Polymorphism, Single Nucleotide MH - Selection, Genetic MH - Phenotype PMC - PMC13704196 OTO - NOTNLM OT - allele pyramiding OT - genomic selection OT - haplotype validation OT - marker-assisted selection OT - pangenome OT - rice breeding COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/10 CRDT- 2026/10/27 10:40 PHST- 2026/07/29 00:00 [received] PHST- 2026/08/22 00:00 [revised] PHST- 2026/08/30 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - ijms27208972 [pii] AID - ijms-27-08972 [pii] AID - 10.3390/ijms27208972 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 10;27(20):8972. doi: 10.3390/ijms27208972. PMID- 42941747 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 9 TI - Feasibility Study of an Assay Measuring Activity of Oral Cancer Protease Mixtures to Cleave Protease-Activated Receptor 2, an Oral Cancer Pain Mediator. LID - 10.3390/ijms27208971 [doi] LID - 8971 AB - Oral cancer patients suffer pain that is patient-specific and heterogeneous at the site of the tumor. Protease-activated receptor 2 (PAR(2)), a G-protein coupled receptor and an oral cancer mediator, is activated by protease cleavage of an N-terminal ligand. Once cleaved, the receptor is retained at the plasma membrane or re-distributed (trafficking) to internal sites followed by downstream protease-specific signaling. The oral cancer tumor microenvironment (TME) is replete with protases that activate PAR(2), potentially evoking PAR(2) trafficking and signaling that is unique to each cancer and reflecting the protease composition of the individual TMEs. To measure PAR(2) cleavage activity in oral cancer samples, an array of overlapping peptides spanning the PAR(2) N-terminus was assembled. In this feasibility study, we assayed the cleavage activity of individual PAR(2)-activating proteases and mixtures of proteases modeling the oral cancer TME. Additionally, we confirmed that PAR(2)-cleaving proteases are active in oral cancer patient saliva, a sample that can be collected non-invasively to measure PAR(2) cleavage in patients when they are experiencing pain prior to surgery. Further studies should elucidate the utility of the peptide array to measure PAR(2)-activating proteolysis in cancer samples. FAU - Ramirez-Garcia, Paulina D AU - Ramirez-Garcia PD AUID- ORCID: 0000-0003-0971-3501 AD - Translational Research Center, NYU Dentistry, New York, NY 10010, USA. FAU - Dolgalev, Igor AU - Dolgalev I AD - Department of Medicine, NYU Langone Health, New York, NY 10016, USA. FAU - Zubli, Sarah AU - Zubli S AD - Translational Research Center, NYU Dentistry, New York, NY 10010, USA. FAU - Schmidt, Brian L AU - Schmidt BL AD - Translational Research Center and the Pain Research Center, NYU Dentistry, New York, NY 10010, USA. FAU - Albertson, Donna G AU - Albertson DG AUID- ORCID: 0000-0002-1433-6483 AD - Translational Research Center and the Pain Research Center, NYU Dentistry, New York, NY 10010, USA. LA - eng GR - P30 CA016087/CA/NCI NIH HHS/United States GR - R01 CA314054/CA/NCI NIH HHS/United States GR - A25-0369-001/Mega-Grants Initiative from the New York University Office of the Provost/ GR - K99 DE033792/DE/NIDCR NIH HHS/United States GR - 5K99DE033792-02A1/NH/NIH HHS/United States GR - 5R01CA231396-05A1/NH/NIH HHS/United States GR - R01 CA231396/CA/NCI NIH HHS/United States GR - 1R01CA314054-01A0/NH/NIH HHS/United States PT - Journal Article DEP - 20261009 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Receptor, PAR-2) RN - 0 (F2RL1 protein, human) RN - EC 3.4.- (Peptide Hydrolases) SB - IM MH - Humans MH - *Receptor, PAR-2/metabolism MH - *Mouth Neoplasms/metabolism/enzymology MH - *Cancer Pain/metabolism MH - Proteolysis MH - Saliva/metabolism/enzymology MH - Feasibility Studies MH - *Peptide Hydrolases/metabolism MH - Tumor Microenvironment PMC - PMC13704349 OTO - NOTNLM OT - MMP1 OT - PAR2 OT - PAR2 N-terminus cleavage OT - PRSS23 OT - ST14 (matriptase) OT - oral cancer OT - protease activity OT - protease-activated receptor 2 OT - saliva OT - trypsin COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/09 CRDT- 2026/10/27 10:40 PHST- 2026/09/14 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - ijms27208971 [pii] AID - ijms-27-08971 [pii] AID - 10.3390/ijms27208971 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 9;27(20):8971. doi: 10.3390/ijms27208971. PMID- 42941745 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 9 TI - Reversibility of Male Factor Infertility: Mechanisms and Evidence from the Literature. LID - 10.3390/ijms27208968 [doi] LID - 8968 AB - Male factor infertility contributes to approximately 50% of reproductive failure in couples worldwide. We conducted a narrative critical review synthesizing evidence from systematic reviews, meta-analyses, randomized controlled trials, and clinical practice guidelines on reversible and irreversible causes of male infertility, examining hormonal, surgical, pharmacological, lifestyle, and drug-induced etiologies. The majority of identifiable etiologies demonstrate some degree of reversibility. Varicocele (prevalence 25-40% in infertile men) achieves natural pregnancy rates of 30-40% after varicocelectomy. Hypogonadotropic hypogonadism responds to gonadotropin therapy with spermatogenesis induction in 90-95% of cases. Obstructive azoospermia achieves ductal patency rates of 85-97% with microsurgical reconstruction. Male accessory gland inflammation is reversible with prompt antibiotic therapy. Spermiotoxic drug exposures-including anabolic-androgenic steroids, sulfasalazine, and chemotherapy-show variable reversibility depending on agent and duration of exposure. Normogonadotropic normotestosteronemic oligozoospermia represents a commonly encountered phenotype that may respond to empirical medical therapy. Lifestyle modifications demonstrate measurable benefits in selected patients. Irreversible causes include Klinefelter syndrome, Y-chromosome AZFa/b microdeletions, and severe post-chemotherapy damage. Systematic andrological evaluation, including comprehensive drug history, is essential to identify treatable causes and guide clinical management. FAU - La Vignera, Sandro AU - La Vignera S AUID- ORCID: 0000-0002-7113-2372 AD - Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, Italy. FAU - Calogero, Aldo E AU - Calogero AE AUID- ORCID: 0000-0001-6950-335X AD - Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, Italy. FAU - Cannarella, Rossella AU - Cannarella R AUID- ORCID: 0000-0003-4599-8487 AD - Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, Italy. FAU - Crafa, Andrea AU - Crafa A AD - Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, Italy. FAU - Barbagallo, Federica AU - Barbagallo F AD - Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, Italy. FAU - Condorelli, Rosita A AU - Condorelli RA AUID- ORCID: 0000-0002-5217-9343 AD - Department of Clinical and Experimental Medicine, University of Catania, 95123 Catania, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261009 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 SB - IM MH - Humans MH - Male MH - *Infertility, Male/etiology/therapy MH - Spermatogenesis MH - Varicocele/complications MH - Azoospermia PMC - PMC13704615 OTO - NOTNLM OT - azoospermia OT - male infertility OT - normogonadotropic normotestosteronemic oligozoospermia OT - reversibility OT - spermiotoxic drugs OT - varicocelectomy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/09 CRDT- 2026/10/27 10:40 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - ijms27208968 [pii] AID - ijms-27-08968 [pii] AID - 10.3390/ijms27208968 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 9;27(20):8968. doi: 10.3390/ijms27208968. PMID- 42941739 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 9 TI - Effects of Experimental Infection with Lagovirus europaeus GI.1 and GI.2 on the Fecal Microbiota of Rabbits: New Insights into Virus-Host Microbiota Associations. LID - 10.3390/ijms27208963 [doi] LID - 8963 AB - Lagovirus europaeus GI.1 (RHDV) and GI.2 (RHDV2), the causative agents of rabbit hemorrhagic disease (RHD), are highly virulent pathogens of the European rabbit; however, their impact on the host fecal microbiota remains poorly understood. Previous field studies have suggested an association between the fecal microbiota and RHDV2 epidemiology in wild rabbits, highlighting the need for studies under controlled experimental conditions. To address this knowledge gap, we investigated the effects of experimental infection with L. europaeus GI.1 and GI.2 on the fecal microbiota of rabbits. Eighteen rabbits were assigned to three groups: an uninfected control group and two groups experimentally infected with L. europaeus GI.1 or GI.2 (n = 6 per group). Fecal microbiota composition was characterized by high-throughput sequencing of the V3-V4 region of the 16S rRNA gene using the HiSeq 2500 platform (Illumina). Rabbits infected with either genotype developed clinical signs consistent with RHD. No significant differences in alpha and beta diversity were observed. However, selective taxonomic shifts were detected, particularly in the GI.2 group, in which the relative abundance of Firmicutes was significantly higher and that of Bacteroidota significantly lower than in the control group. The abundance of Muribaculaceae was lower in both infected groups, whereas Sporosarcina reached its highest abundance in the GI.2 group. Correlation analysis revealed associations between selected bacterial taxa and clinical parameters. Muribaculaceae abundance was positively correlated with survival time and negatively correlated with lethargy and diarrhea, whereas Sporosarcina showed the opposite pattern, with positive correlations with lethargy and diarrhea and a negative correlation with survival time. Our findings indicate that experimental infection with L. europaeus GI.1 and GI.2 was not associated with a global restructuring of the fecal microbiota but rather with selective changes in the abundance of specific bacterial taxa, some of which were associated with viral genotype and the clinical course of infection. To our knowledge, this is the first controlled experimental study to directly compare the effects of L. europaeus GI.1 and GI.2 infection on the rabbit fecal microbiota. This study extends previous field observations by providing experimental evidence and offers new insights into the associations between L. europaeus infection and the host fecal microbiota during RHD. FAU - Hukowska-Szematowicz, Beata AU - Hukowska-Szematowicz B AUID- ORCID: 0000-0002-2776-2799 AD - Laboratory of Infectious Biology, Molecular Biology and Immunology, Institute of Biology, University of Szczecin, st. Waska 13, 71-415 Szczecin, Poland. AD - Molecular Biology and Biotechnology Center, University of Szczecin, st. Waska 13, 71-415 Szczecin, Poland. FAU - Fitzner, Andrzej AU - Fitzner A AUID- ORCID: 0000-0001-5272-6004 AD - Department of Virology and Viral Animal Disease, National Veterinary Research Institute-State Research Institute, st. Wodna 7, 98-220 Zdunska Wola, Poland. AD - National Reference Laboratory for Rabbit Hemorrhagic Disease (RHD), st. Wodna 7, 98-220 Zdunska Wola, Poland. FAU - Mazgaj, Szymon AU - Mazgaj S AUID- ORCID: 0009-0007-9661-6537 AD - Laboratory of Infectious Biology, Molecular Biology and Immunology, Institute of Biology, University of Szczecin, st. Waska 13, 71-415 Szczecin, Poland. AD - Molecular Biology and Biotechnology Center, University of Szczecin, st. Waska 13, 71-415 Szczecin, Poland. AD - Doctoral School, University of Szczecin, st. A. Mickiewicz 16, 70-384 Szczecin, Poland. FAU - Dybus, Andrzej AU - Dybus A AD - Department of Genetics, West Pomeranian University of Technology in Szczecin, st. Aleja Piastow 45, 70-311 Szczecin, Poland. FAU - Kulig, Hanna AU - Kulig H AUID- ORCID: 0000-0003-1653-3453 AD - Department of Genetics, West Pomeranian University of Technology in Szczecin, st. Aleja Piastow 45, 70-311 Szczecin, Poland. FAU - Hsiao, Felix Shih-Hsiang AU - Hsiao FS AUID- ORCID: 0000-0002-4401-5146 AD - Department of Biotechnology and Animal Science, National Ilan University, No. 1, Sec. 1, Shennong Rd., Yilan City 26047, Taiwan. FAU - Kosik-Bogacka, Danuta AU - Kosik-Bogacka D AUID- ORCID: 0000-0002-3796-5493 AD - Department of Biology, Parasitology and Pharmaceutical Botany, Pomeranian Medical University in Szczecin, Powstancow Wielkopolskich 72, 70-204 Szczecin, Poland. FAU - Li, Yi-Hung AU - Li YH AD - Department of Biotechnology and Animal Science, National Ilan University, No. 1, Sec. 1, Shennong Rd., Yilan City 26047, Taiwan. FAU - Yu, Yu-Hsiang AU - Yu YH AUID- ORCID: 0000-0001-9629-8478 AD - Department of Biotechnology and Animal Science, National Ilan University, No. 1, Sec. 1, Shennong Rd., Yilan City 26047, Taiwan. LA - eng GR - Minister of Science under the "Regional Excellence Initiative" Program. Agreement No. RID/SP/0045/2024/01. Agreement No. RID/SP/0045/2024/01./Minister of Science/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (RNA, Ribosomal, 16S) SB - IM MH - Animals MH - Rabbits MH - *Feces/microbiology/virology MH - *Caliciviridae Infections/veterinary/virology/microbiology MH - RNA, Ribosomal, 16S/genetics MH - *Lagovirus/pathogenicity/physiology/genetics MH - Hemorrhagic Disease Virus, Rabbit MH - *Gastrointestinal Microbiome PMC - PMC13704132 OTO - NOTNLM OT - 16S rRNA OT - Lagovirus europaeus OT - RHD OT - RHDV OT - RHDV2 OT - fecal microbiota OT - rabbit hemorrhagic disease OT - rabbits COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/09 CRDT- 2026/10/27 10:40 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - ijms27208963 [pii] AID - ijms-27-08963 [pii] AID - 10.3390/ijms27208963 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 9;27(20):8963. doi: 10.3390/ijms27208963. PMID- 42941734 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 9 TI - Genetic and Immunologic Interactions Driving STAT3 Signaling and Macrophage Polarization in Acute Myeloid Leukemia. LID - 10.3390/ijms27208957 [doi] LID - 8957 AB - Acute myeloid leukemia (AML) is an increasingly recognized malignancy sustained by a dysregulated inflammatory niche, in which genetic alterations and microenvironmental signaling converge to drive pathogenesis. This review synthesizes emerging evidence linking genetic mutations and immune dysregulation in AML, proposing an integrated mutation-macrophage-signal transducer and activator of transcription 3 (STAT3) axis as a conceptual framework of disease progression. It proposes that mutations in CCAAT/enhancer-binding protein alpha (CEBPA) and Runt-related transcription factor 1 (RUNX1), despite conferring divergent genetic risk, converge on a common pathway of inflammatory cytokine overproduction, including interleukin-1 beta (IL-1beta), tumor necrosis factor alpha (TNF-alpha), interleukin-6 (IL-6), and interleukin-10 (IL-10), as well as C-C motif chemokine ligand 2 (CCL2)-mediated recruitment of tumor-associated macrophages. These educated macrophages are proposed to undergo M2-like polarization, releasing oncostatin M (OSM) and IL-6, which are positioned to activate the Janus kinase/signal transducer and activator of transcription 3 (JAK/STAT3) survival pathway in leukemic blasts. Concurrently, IL-10 produced by regulatory T cells, stromal cells, and M2-like polarized macrophages within the leukemic niche signals through its cognate receptor to potently activate STAT3, suppressing antigen-presenting cell function, downregulating major histocompatibility complex class II (MHC class II) expression, and impairing effective anti-tumor T-cell immunity, thereby potentially reinforcing the immunosuppressive architecture that shields leukemic blasts from immune surveillance. This proposed feed-forward loop is associated with transcriptional upregulation of the anti-apoptotic factor BCL2-like 1 (BCL2L1) and the negative-feedback regulator suppressor of cytokine signaling 3 (SOCS3), a pattern consistent with chemoresistance and impaired negative-feedback control of STAT3 signaling. Translational biomarkers, including OSM, IL-6, IL-10, and SOCS3/BCL2L1 transcripts, may quantify this axis, offering a dynamic, functional complement to static genetic risk stratification. Ultimately, this proposed triad suggests that aberrant inflammation may represent a shared therapeutic vulnerability; targeting the IL-6/oncostatin M receptor (OSMR)/STAT3 signaling nodes or reprogramming macrophage polarization could, if validated, disrupt this circuit in selected mutational subtypes. FAU - Ahmed, Kvan Omar AU - Ahmed KO AUID- ORCID: 0000-0003-0223-812X AD - Department of Biology, College of Education for Pure Science, Tikrit University, Tikrit 34001, Iraq. AD - Department of Medical Microbiology, College of Medicine, University of Kirkuk, Kirkuk 36001, Iraq. FAU - Ahmed, Measer Abdullah AU - Ahmed MA AUID- ORCID: 0000-0002-0157-6138 AD - Department of Biology, College of Education for Pure Science, Tikrit University, Tikrit 34001, Iraq. FAU - Othman, Goran AU - Othman G AUID- ORCID: 0000-0002-0880-042X AD - Department of Medical Laboratory Technology, Erbil Health and Medical Technical College, Erbil Polytechnic University, Erbil 44001, Kurdistan Region, Iraq. AD - Department of Biology, College of Science, Al-Qalam University, Kirkuk 36001, Iraq. FAU - Smail, Shukur Wasman AU - Smail SW AD - College of Pharmacy, Cihan University-Erbil, Erbil 44001, Kurdistan Region, Iraq. AD - Department of Biology, College of Science, Salahaddin University-Erbil, Erbil 44001, Kurdistan Region, Iraq. AD - Department of Medical Science, Respiratory Medicine, and Allergology, Uppsala University and University Hospital, 75185 Uppsala, Sweden. LA - eng PT - Journal Article PT - Review DEP - 20261009 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (STAT3 Transcription Factor) RN - 0 (STAT3 protein, human) RN - 0 (Cytokines) SB - IM MH - Humans MH - *Leukemia, Myeloid, Acute/genetics/immunology/metabolism/pathology MH - *STAT3 Transcription Factor/metabolism/genetics MH - *Signal Transduction MH - *Macrophages/immunology/metabolism MH - Animals MH - Mutation MH - Cytokines/metabolism PMC - PMC13704360 OTO - NOTNLM OT - CCAAT/enhancer-binding protein alpha OT - acute myeloid leukemia OT - inflammatory cytokines OT - oncostatin M OT - runt-related transcription factor 1 OT - signal transducer and activator of transcription 3 signaling OT - tumor-associated macrophages COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/09 CRDT- 2026/10/27 10:40 PHST- 2026/08/01 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - ijms27208957 [pii] AID - ijms-27-08957 [pii] AID - 10.3390/ijms27208957 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 9;27(20):8957. doi: 10.3390/ijms27208957. PMID- 42941733 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 9 TI - Angiogenic Imbalance in Early Placental Dysfunction: Pro- and Anti-Angiogenic Factors, Pathophysiology, Screening and Therapeutic Horizons. LID - 10.3390/ijms27208958 [doi] LID - 8958 AB - Placental dysfunction is a major component of several pregnancy complications, particularly preeclampsia and fetal growth restriction (FGR). These disorders could be presented with different phenotypes based on abnormal placentation and inadequate maternal vascular adaptation, which are baseline mechanisms in affected pregnancies. Defective trophoblast invasion and incomplete transformation of the maternal spiral arteries may produce a high-resistance uteroplacental circulation, resulting in placental hypo-perfusion, ischemia-reperfusion injury, oxidative stress and subsequent release of factors that affect maternal endothelial function. The aim of this review is to summarize the current understanding of pro-angiogenic and anti-angiogenic factors in early placental dysfunction, with special emphasis in their biological function and molecular roles, relationship with abnormal placentation and clinical significance during pregnancy. The development of placental dysfunction is generally understood as a multifactorial and multistep process. The literature evidence most often supports the three-step process of placenta dysfunction. The angiogenic imbalance may precede the clinical presentation of preeclampsia and may also be associated with fetal growth restriction. Among currently available biomarkers, the sFlt-1/PlGF ratio has the strongest clinical evidence for risk stratification in women with suspected preeclampsia. Nevertheless, the role of angiogenic factors in the earliest stages of placental dysfunction remains incompletely defined. The major challenge is to determine whether angiogenic abnormalities can identify clinically meaningful placental dysfunction early enough to permit effective intervention. This suggests that pro-angiogenic therapeutics could be a possible option for the treatment of pre-eclampsia, but also a more specific and sensitive predictive and diagnostic tool in modern obstetrics. FAU - Nikolic Turnic, Tamara AU - Nikolic Turnic T AD - Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. AD - N.A. Semashko Public Health and Healthcare Department, F.F. Erismann Institute of Public Health, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119048 Moscow, Russia. AD - Center of Excellence for Redox Balance Research in Cardiovascular and Metabolic Disorders, 34000 Kragujevac, Serbia. FAU - Aksentijevic, Marija AU - Aksentijevic M AD - Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000 Kragujevac, Serbia. LA - eng PT - Journal Article PT - Review DEP - 20261009 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Angiogenesis Inducing Agents) RN - 0 (Biomarkers) SB - IM MH - Humans MH - Female MH - Pregnancy MH - *Placenta/physiopathology/metabolism/pathology MH - Pre-Eclampsia/metabolism/physiopathology MH - *Angiogenesis MH - Fetal Growth Retardation/metabolism MH - *Placenta Diseases/metabolism/diagnosis/physiopathology MH - Animals MH - *Angiogenesis Inducing Agents/metabolism MH - Biomarkers/metabolism PMC - PMC13704371 OTO - NOTNLM OT - PIGF OT - TGF-beta OT - VEGF OT - angiogenic factors OT - eEng OT - placental dyfunction OT - sFlt-1 OT - sFlt-1/PlGF ratio COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/09 CRDT- 2026/10/27 10:40 PHST- 2026/08/17 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - ijms27208958 [pii] AID - ijms-27-08958 [pii] AID - 10.3390/ijms27208958 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 9;27(20):8958. doi: 10.3390/ijms27208958. PMID- 42941729 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261029 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 9 TI - The Proliferation-Invasion Dichotomy: Transcriptomic Signatures of lncRNA and Ubiquitin-Proteasome Networks in Hypermutated Endometrial Cancer. LID - 10.3390/ijms27208954 [doi] LID - 8954 AB - Endometrial cancer incidence is markedly rising globally, posing an urgent need to elucidate the molecular mechanisms driving disease characterization and progression. Endometrial cancer evolution is driven by canonical Wnt and p53 pathway alterations, yet the mechanisms orchestrating the transition from localized proliferation to stromal invasion, the proliferation-invasion dichotomy, remain poorly understood. While long non-coding RNAs (lncRNAs) are emerging as critical correlates of divergent tumor phenotypes, their coordination with the broader ubiquitin-proteasome system (UPS), specifically E3 ligase and deubiquitinase (DUB) networks, during endometrial cancer progression is largely unexplored. Whole-transcriptome RNA sequencing was performed on formalin-fixed paraffin-embedded (FFPE) tissue from a clinical cohort of 38 patients, comprising 34 primary endometrial carcinomas across distinct molecular subtypes (MSI, POLE, p53abn, and NSMP) and four pre-cancerous endometrial intraepithelial neoplasia (EIN) lesions. To stringently control for baseline patient-specific confounding, differential gene expression and principal component analyses were executed using a strict, intra-patient, matched-pair design (Tumor vs. Adjacent Normal). A principal component analysis of the batch-corrected transcriptome revealed a profound divergence between tumor and paired adjacent normal phenotypes. This spatial divergence was heavily characterized by lncRNA signatures (including SOCS2-AS1) strongly associated with extracellular matrix (ECM) remodeling. Concurrently, we identified a critical, malignancy-specific transcriptomic dysregulation across the broader UPS network. Within this altered transcriptional landscape, the ubiquitin C-terminal hydrolase (UCHL) family emerged as a highly significant node. UCHL5 displayed robust, global upregulation across the pan-cancer cohort compared with matched controls (log(2)FC = 0.83, padj < 0.001). Strikingly, UCHL3 exhibited global upregulation (log(2)FC = 1.23, padj = 0.002) that was highly concentrated within the microsatellite instability (MSI) and polymerase epsilon (POLE) hypermutated subtypes, highlighting a potential compensatory transcriptomic response to the predicted cellular stress inherent to high mutational burdens. In an exploratory subset of pre-cancerous EIN lesions (n = 4), neither the invasive lncRNA networks nor profound UPS/UCHL dysregulation were observed, suggesting a preliminary trend that requires validation in larger precursor cohorts. Our paired transcriptomic mapping reveals that endometrial cancer progression is characterized by divergent transcriptomic programs: specific lncRNA networks are tightly coupled to the invasive remodeling of the ECM, while concurrent dysregulation of broad UPS networks reflects the altered proteostasis associated with this phenotypic shift. The pronounced upregulation of critical UPS nodes, such as UCHL3 in MSI/POLE tumors, highlights a landscape of novel, enzymatically targetable vulnerabilities in hypermutated endometrial cancers. FAU - Casanova, Joao AU - Casanova J AD - Gynecologic Oncology Unit, Department of Obstetrics and Gynecology, Hospital da Luz Lisboa, Luz Saude, Avenida Lusiada 100, 1500-650 Lisboa, Portugal. FAU - Costa, Ana Gomes da AU - Costa AGD AD - Gynecologic Oncology Unit, Department of Obstetrics and Gynecology, Hospital da Luz Lisboa, Luz Saude, Avenida Lusiada 100, 1500-650 Lisboa, Portugal. FAU - Tripepi, Marta AU - Tripepi M AD - Unit of Gynecology and Obstetrics, Department of Women and Children, University of Padua, 35122 Padua, Italy. FAU - Pestana, Ana AU - Pestana A AD - Department of Pathology, Hospital da Luz Lisboa, Luz Saude, Avenida Lusiada 100, 1500-650 Lisboa, Portugal. FAU - Catarino, Ana AU - Catarino A AD - Department of Pathology, Hospital da Luz Lisboa, Luz Saude, Avenida Lusiada 100, 1500-650 Lisboa, Portugal. FAU - Martins, Nelson AU - Martins N AD - Catolica Biomedical Research Centre, Faculdade de Medicina, Universidade Catolica Portuguesa, 2780-156 Oeiras, Portugal. FAU - Teixeira, Luis AU - Teixeira L AD - Catolica Biomedical Research Centre, Faculdade de Medicina, Universidade Catolica Portuguesa, 2780-156 Oeiras, Portugal. FAU - Abu-Rustum, Nadeem AU - Abu-Rustum N AUID- ORCID: 0000-0001-9689-1298 AD - Gynecology Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, Weill Medical College of Cornell University, 1275 York Avenue, New York, NY 10065, USA. FAU - Rosado-Ramos, Rita AU - Rosado-Ramos R AD - Hospital da Luz Learning Health, Luz Saude, Avenida Lusiada 100, 1500-650 Lisboa, Portugal. FAU - Matthiensen, Rune AU - Matthiensen R AUID- ORCID: 0000-0002-6353-2616 AD - iNOVA4Health, NOVA Medical School, Faculdade de Ciencias Medicas, Universidade NOVA de Lisboa, 1169-056 Lisboa, Portugal. FAU - Lima, Jorge AU - Lima J AUID- ORCID: 0000-0002-0831-2741 AD - Department of Obstetrics and Gynecology, Hospital da Luz Lisboa, Luz Saude, Avenida Lusiada 100, 1500-650 Lisboa, Portugal. AD - Comprehensive Health Research Centre (CHRC), NOVA Medical School (NMS), Universidade NOVA de Lisboa, Campo Martires da Patria, 130, 1169-056 Lisboa, Portugal. LA - eng GR - P30 CA008748/CA/NCI NIH HHS/United States GR - Women s Health and Maternal Fetal Research Group/Luz Saude SA/ GR - P30CA008748/CA/NCI NIH HHS/United States PT - Journal Article DEP - 20261009 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - EC 3.4.25.1 (Proteasome Endopeptidase Complex) RN - 0 (Ubiquitin) RN - 0 (RNA, Long Noncoding) SB - IM MH - Humans MH - Female MH - *Endometrial Neoplasms/genetics/pathology/metabolism MH - *Proteasome Endopeptidase Complex/genetics/metabolism MH - Gene Expression Regulation, Neoplastic MH - *Ubiquitin/metabolism/genetics MH - *RNA, Long Noncoding/genetics MH - *Transcriptome MH - Cell Proliferation/genetics MH - Neoplasm Invasiveness MH - Gene Expression Profiling MH - Mutation PMC - PMC13704094 OTO - NOTNLM OT - POLE hypermutation OT - deubiquitinase (DUB) OT - endometrial cancer OT - microsatellite instability (MSI) OT - proteostasis OT - ubiquitin-proteasome system (UPS) COIS- Dr. Abu-Rustum received research funding paid to the institution from GRAIL. Memorial Sloan Kettering Cancer Center also has equity in GRAIL. All the other authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/09 CRDT- 2026/10/27 10:40 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - ijms27208954 [pii] AID - ijms-27-08954 [pii] AID - 10.3390/ijms27208954 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 9;27(20):8954. doi: 10.3390/ijms27208954. PMID- 42941728 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 9 TI - Single-Cell BCR and Transcriptome Analysis Revealed Potent Neutralizing Antibodies in Response to HAdV-4 Infection. LID - 10.3390/ijms27208952 [doi] LID - 8952 AB - Human adenovirus type 4 (HAdV-4) is a predominant serotype causing acute respiratory infections, for which no antibody drugs have been clinically approved to date, resulting in an urgent unmet medical need. This study aimed to identify highly potent neutralizing antibodies by dissecting the B cell immune response after infection, thereby laying a foundation for vaccine design and antiviral therapy. Peripheral blood mononuclear cells (PBMCs) were isolated from a convalescent patient who exhibited the most robust neutralizing antibody titers among 16 HAdV-4 convalescent volunteers. A total of 2710 B cells were isolated from the peripheral blood of this patient, and the characteristics of B cell subsets and B cell receptor (BCR) repertoires were comprehensively analyzed by integrating scRNA-seq and scVDJ-seq technologies. Candidate antibodies were subsequently selected for a series of functional and mechanistic validations based on the characteristics of BCRs and origination. Transcriptomic profiling classified memory B cells into three functionally heterogeneous subpopulations designated as MB sub1, sub2 and sub3. Combined with the characteristics of BCR, 92 selected antibodies from sub2 and sub3 were synthesized for further functional validation. Three potent neutralizing antibodies were successfully identified, among which 1QH7 demonstrated potentially potent virus neutralization capability in vitro and conferred robust in vivo protection in the Stat1-deficient mouse model. We further employed AlphaFold 3 to predict the complex structure of 1QH7 with the HAdV-4 Hexon protein and validated the key binding residues through site-directed mutagenesis. Additionally, the study elucidated the viral neutralization mechanism mediated by TRIM21. We established an efficient antibody screening strategy based on integration of scRNA-seq and scVDJ-seq and isolated a potent neutralizing antibody, 1QH7. This study lays a solid foundation for the rapid pathogen-bait-free, cell subset-guided screening antibody route for novel emerging infectious disease outbreaks. FAU - Yu, Tingting AU - Yu T AD - National Key Laboratory of Advanced Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. FAU - Ye, Xiaolei AU - Ye X AD - National Key Laboratory of Advanced Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. FAU - Zhu, Zheng AU - Zhu Z AD - National Key Laboratory of Advanced Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. FAU - Zhang, Wanrong AU - Zhang W AD - National Key Laboratory of Advanced Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. FAU - Yang, You AU - Yang Y AD - National Key Laboratory of Advanced Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. FAU - Wang, Jianrong AU - Wang J AD - National Key Laboratory of Advanced Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. FAU - Lv, Peng AU - Lv P AD - National Key Laboratory of Advanced Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. FAU - Chi, Xiangyang AU - Chi X AD - National Key Laboratory of Advanced Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. FAU - Zhang, Guanying AU - Zhang G AD - National Key Laboratory of Advanced Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. FAU - Chen, Zhengshan AU - Chen Z AUID- ORCID: 0000-0003-2869-1467 AD - National Key Laboratory of Advanced Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. FAU - Dong, Yunzhu AU - Dong Y AD - National Key Laboratory of Advanced Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China. LA - eng PT - Journal Article DEP - 20261009 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Antibodies, Neutralizing) RN - 0 (Antibodies, Viral) RN - 0 (Receptors, Antigen, B-Cell) SB - IM MH - *Antibodies, Neutralizing/immunology MH - Humans MH - Animals MH - *Antibodies, Viral/immunology MH - Mice MH - *Adenoviruses, Human/immunology MH - *Receptors, Antigen, B-Cell/genetics/immunology MH - Single-Cell Analysis MH - Gene Expression Profiling MH - Single-Cell Gene Expression Analysis MH - Female MH - B-Lymphocytes/immunology PMC - PMC13704714 OTO - NOTNLM OT - human adenovirus serotype 4 OT - neutralizing monoclonal antibody OT - single-cell sequencing COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/09 CRDT- 2026/10/27 10:40 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - ijms27208952 [pii] AID - ijms-27-08952 [pii] AID - 10.3390/ijms27208952 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 9;27(20):8952. doi: 10.3390/ijms27208952. PMID- 42941718 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 27 IP - 20 DP - 2026 Oct 9 TI - Reduced DDB2 Expression Is Associated with Temozolomide Resistance in Glioblastoma Cells. LID - 10.3390/ijms27208941 [doi] LID - 8941 AB - Glioblastoma (GBM) remains one of the most lethal primary brain tumours, and resistance to temozolomide (TMZ), the standard chemotherapeutic agent for GBM, represents a major obstacle to effective treatment. Although O(6)-methylguanine-DNA methyltransferase (MGMT) is a well-established determinant of TMZ response, MGMT status alone does not fully explain the heterogeneity of treatment outcomes, suggesting the involvement of additional DNA damage response mechanisms. Here, we investigated the contribution of different DNA repair pathways to TMZ sensitivity in a panel of GBM cell lines displaying distinct TMZ response profiles. Expression analysis of 84 DNA repair genes identified damage-specific DNA binding protein 2 (DDB2) as the only one consistently associated with TMZ sensitivity. We found that DDB2 expression was significantly reduced in TMZ-resistant cell lines and selectively induced following TMZ exposure in TMZ-sensitive cells. Proteomic profiling of representative sensitive and resistant cell models further revealed distinct responses to TMZ and identified enrichment of p53-associated DNA damage response pathways in sensitive cells, including altered expression of DDB2 and other canonical p53-regulated proteins. Importantly, siRNA-mediated depletion of DDB2 in the TMZ-sensitive A172 GBM cell line significantly reduced TMZ sensitivity. These findings provide functional evidence that DDB2 contributes to the cellular response to TMZ and extend the established roles of DDB2 in DNA repair and cancer biology to TMZ responsiveness in GBM. FAU - Gravalos-Cano, Ines AU - Gravalos-Cano I AUID- ORCID: 0000-0002-0128-0513 AD - Department of Genetics, University of Cordoba, 14014 Cordoba, Spain. AD - Maimonides Biomedical Research Institute of Cordoba (IMIBIC), 14004 Cordoba, Spain. FAU - Morales-Ruiz, Teresa AU - Morales-Ruiz T AUID- ORCID: 0000-0002-6357-6977 AD - Department of Genetics, University of Cordoba, 14014 Cordoba, Spain. AD - Maimonides Biomedical Research Institute of Cordoba (IMIBIC), 14004 Cordoba, Spain. FAU - Ariza, Rafael R AU - Ariza RR AUID- ORCID: 0000-0002-0338-7306 AD - Department of Genetics, University of Cordoba, 14014 Cordoba, Spain. AD - Maimonides Biomedical Research Institute of Cordoba (IMIBIC), 14004 Cordoba, Spain. FAU - Roldan-Arjona, Teresa AU - Roldan-Arjona T AUID- ORCID: 0000-0003-2842-3956 AD - Department of Genetics, University of Cordoba, 14014 Cordoba, Spain. AD - Maimonides Biomedical Research Institute of Cordoba (IMIBIC), 14004 Cordoba, Spain. FAU - Martinez-Macias, Maria Isabel AU - Martinez-Macias MI AD - Department of Genetics, University of Cordoba, 14014 Cordoba, Spain. AD - Maimonides Biomedical Research Institute of Cordoba (IMIBIC), 14004 Cordoba, Spain. LA - eng GR - PID2019-109967 and GB-I00PID2022-140458NB-I00/Ministerio de Ciencia, Innovacion y Universidades/ GR - Marie Sklodowska-Curie grant agreement No 847468/European Union/ GR - Junta de Andalucia/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - YF1K15M17Y (Temozolomide) RN - 0 (DNA-Binding Proteins) RN - 0 (DDB2 protein, human) RN - 0 (Antineoplastic Agents, Alkylating) SB - IM MH - Humans MH - *Temozolomide/pharmacology MH - *Glioblastoma/genetics/metabolism/drug therapy/pathology MH - *Drug Resistance, Neoplasm/genetics MH - *DNA-Binding Proteins/genetics/metabolism MH - Cell Line, Tumor MH - *Antineoplastic Agents, Alkylating/pharmacology MH - Gene Expression Regulation, Neoplastic/drug effects MH - *Brain Neoplasms/genetics/metabolism/drug therapy MH - DNA Damage MH - DNA Repair/drug effects PMC - PMC13704723 OTO - NOTNLM OT - DDB2 OT - DNA damage OT - DNA repair OT - glioblastoma OT - temozolomide COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/09 CRDT- 2026/10/27 10:40 PHST- 2026/07/08 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - ijms27208941 [pii] AID - ijms-27-08941 [pii] AID - 10.3390/ijms27208941 [doi] PST - epublish SO - Int J Mol Sci. 2026 Oct 9;27(20):8941. doi: 10.3390/ijms27208941. PMID- 42941712 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Real-World Treatment Patterns and Persistence of Omidenepag Isopropyl in Korean Patients with Normal-Tension Glaucoma. LID - 10.3390/jcm15208200 [doi] LID - 8200 AB - Background/Objectives: Prostaglandin analogues may cause prostaglandin-associated periorbitopathy (PAP). Omidenepag isopropyl (OMDI), a selective EP2 receptor agonist, may provide an alternative, but real-world data in normal-tension glaucoma (NTG) are limited. We evaluated treatment patterns, persistence, and intraocular pressure (IOP) outcomes of OMDI in Korean patients with NTG. Methods: This retrospective study included 403 eyes of 218 patients with NTG who initiated OMDI and were followed for up to 12 months. Patients received monotherapy, adjunctive therapy, or switching therapy. IOP was analyzed using a three-level linear mixed-effects model, and persistence was assessed using Kaplan-Meier analysis. Results: OMDI was prescribed as monotherapy in 88 patients (40.4%), adjunctive therapy in 25 (11.5%), and switching therapy in 105 (48.2%). Among switchers, PAP was the most common reason (68.6%). IOP decreased significantly in all groups (all p < 0.001), with model-estimated 12-month changes of -4.08, -3.37, and -1.40 mmHg, respectively. Twelve-month persistence was 79.8%. OMDI was discontinued in 44 patients (20.2%), most commonly because of visual disturbance or decreased vision (38.6%). Conclusions: OMDI provided sustained IOP reduction and 79.8% 12-month persistence in Korean patients with NTG and was frequently selected as an alternative to prostaglandin analogues in patients with PAP. FAU - Kim, Na Hee AU - Kim NH AD - Department of Ophthalmology, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul 05030, Republic of Korea. FAU - Jo, Youn Hye AU - Jo YH AD - Department of Ophthalmology, Konkuk University Medical Center, Konkuk University School of Medicine, Seoul 05030, Republic of Korea. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705832 OTO - NOTNLM OT - EP2 receptor agonist OT - normal-tension glaucoma OT - omidenepag isopropyl OT - prostaglandin-associated periorbitopathy OT - real-world evidence OT - treatment persistence COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:40 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - jcm15208200 [pii] AID - jcm-15-08200 [pii] AID - 10.3390/jcm15208200 [doi] PST - epublish SO - J Clin Med. 2026 Oct 21;15(20):8200. doi: 10.3390/jcm15208200. PMID- 42941710 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Association of Lower-Limb Asymmetry with Falls, Physical Performance and Frailty in Older Adults: A Systematic Review. LID - 10.3390/jcm15208198 [doi] LID - 8198 AB - Background/Objectives: Whether lower-limb interlimb asymmetry is associated with adverse outcomes in older adults independently of bilateral strength, power or muscle mass remains unclear. This review synthesized evidence linking lower-limb asymmetry with falls, physical performance and frailty in adults aged >/= 60 years from populations not defined by a unilateral neurological or musculoskeletal condition. Methods: Following PRISMA 2020, PubMed, Scopus, Web of Science and EBSCOhost were searched on 2 August 2026 for observational studies quantifying lower-limb asymmetry. Risk of bias was assessed with the Newcastle-Ottawa Scale and AXIS. Because exposure definitions and effect measures were incompatible, meta-analysis was not feasible; a narrative synthesis based on the direction of effect was conducted according to SWiM, and certainty of evidence was rated with GRADE. Results: Twenty-six studies (10,167 participants; 16 cross-sectional, 6 case-control, 4 prospective cohorts) were included. Findings for strength and power asymmetry were inconsistent, and the largest prospective cohort (n = 5347) found no association with recurrent falls. Greater muscle mass asymmetry was associated with slower gait speed in three cross-sectional studies. Selected gait and loading asymmetry measures were associated with subsequent falls and incident frailty in two small cohorts. Positive findings for falls came mainly from case-control studies with retrospective, self-reported fall ascertainment, whereas the studies at the lowest risk of bias did not confirm them. Certainty of evidence was very low for all eight exposure-outcome pairs, including muscle mass asymmetry and gait speed. Conclusions: Lower-limb asymmetry is not a homogeneous construct, and its associations, which depend on the measurement domain and outcome, should be regarded as exploratory. Current evidence does not establish the independent predictive value of asymmetry and supports neither a universal asymmetry threshold nor its use as a standalone fall-risk marker; it should be interpreted alongside bilateral muscle capacity and task demands. FAU - Kasicki, Krzysztof AU - Kasicki K AUID- ORCID: 0009-0008-9231-8353 AD - Department of Sport Theory and Motor Skills, Institute of Sports Sciences, University of Physical Culture in Krakow, 31-571 Krakow, Poland. FAU - Szymeczko, Kacper AU - Szymeczko K AUID- ORCID: 0009-0002-1235-7720 AD - Faculty of Health Sciences, Andrzej Frycz Modrzewski Krakow University, 30-705 Krakow, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706116 OTO - NOTNLM OT - fall risk OT - frailty OT - functional tests OT - gait OT - interlimb asymmetry OT - older adults OT - physical fitness OT - strength COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:40 PHST- 2026/09/28 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - jcm15208198 [pii] AID - jcm-15-08198 [pii] AID - 10.3390/jcm15208198 [doi] PST - epublish SO - J Clin Med. 2026 Oct 21;15(20):8198. doi: 10.3390/jcm15208198. PMID- 42941709 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Morphology-Guided Diagnosis of Triple-Negative Breast Carcinoma: From Histopathological Evaluation to Molecular Testing. LID - 10.3390/jcm15208197 [doi] LID - 8197 AB - Background: Triple-negative breast carcinoma (TNBC) comprises several histological entities with different molecular alterations and clinical behaviour. Recognising these entities is important for accurate classification and for choosing appropriate immunohistochemical and molecular tests. Methods: We conducted a narrative review of English-language literature identified through PubMed/MEDLINE, Scopus, Web of Science Core Collection, citation searching and a Scopus publication list. Journal publications available through 24 August 2026 were eligible. Current reporting and regulatory guidance was obtained from official websites. Findings were synthesised qualitatively, without statistical pooling. Results: The review included 93 journal publications and two official documents. Secretory carcinoma, adenoid cystic carcinoma, tall cell carcinoma with reversed polarity and metaplastic carcinoma require a different diagnostic approach from conventional high-grade invasive breast carcinoma of no special type. Architectural, cytological and stromal findings guide the differential diagnosis and the selection of immunohistochemical stains and molecular assays. Limited sampling, tumour heterogeneity and treatment-related changes can affect tissue selection and interpretation. Conclusions: Pathology reports should specify the histological type and clearly state any remaining diagnostic uncertainty. Morphology should guide diagnostic testing, while validated predictive biomarkers and clinical criteria determine treatment eligibility. FAU - Zuccala, Valeria AU - Zuccala V AUID- ORCID: 0000-0003-3478-9217 AD - Department of Human Pathology of Adults and Developmental Age "Gaetano Barresi", Division of Pathology, University of Messina, Via Consolare Valeria 1, 98125 Messina, Italy. FAU - Rizzuto, Ida AU - Rizzuto I AUID- ORCID: 0009-0001-1234-3030 AD - Department of Human Pathology of Adults and Developmental Age "Gaetano Barresi", Division of Pathology, University of Messina, Via Consolare Valeria 1, 98125 Messina, Italy. FAU - Pepe, Ludovica AU - Pepe L AD - Department of Human Pathology of Adults and Developmental Age "Gaetano Barresi", Division of Pathology, University of Messina, Via Consolare Valeria 1, 98125 Messina, Italy. AD - Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, Via Consolare Valeria 1, 98125 Messina, Italy. FAU - Martorana, Teresa Maria AU - Martorana TM AD - Department of Human Pathology of Adults and Developmental Age "Gaetano Barresi", Division of Pathology, University of Messina, Via Consolare Valeria 1, 98125 Messina, Italy. FAU - Fiorentino, Vincenzo AU - Fiorentino V AUID- ORCID: 0000-0002-1132-1761 AD - Department of Human Pathology of Adults and Developmental Age "Gaetano Barresi", Division of Pathology, University of Messina, Via Consolare Valeria 1, 98125 Messina, Italy. AD - Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, Via Consolare Valeria 1, 98125 Messina, Italy. FAU - Tralongo, Pietro AU - Tralongo P AUID- ORCID: 0000-0003-4957-3283 AD - Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, Via Consolare Valeria 1, 98125 Messina, Italy. FAU - Ricciardi, Gabriele AU - Ricciardi G AUID- ORCID: 0009-0002-1349-0910 AD - Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, Via Consolare Valeria 1, 98125 Messina, Italy. AD - Istituto Clinico Polispecialistico C.O.T. Cure Ortopediche Traumatologiche S.p.A., Via Ducezio 1, 98124 Messina, Italy. FAU - Ciappina, Giuliana AU - Ciappina G AD - Applied Biology and Experimental Medicine, Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, Italy. FAU - Arico, Francesco Marcello AU - Arico FM AUID- ORCID: 0000-0002-4230-8332 AD - BIOMORF Department, Oncologic Imaging Division, University Hospital Policlinico G. Martino, University of Messina, Via Consolare Valeria 1, 98125 Messina, Italy. FAU - Marino, Maria Adele AU - Marino MA AD - BIOMORF Department, Oncologic Imaging Division, University Hospital Policlinico G. Martino, University of Messina, Via Consolare Valeria 1, 98125 Messina, Italy. FAU - Berretta, Massimiliano AU - Berretta M AUID- ORCID: 0000-0002-9837-9148 AD - Medical Oncology Unit, Department of Clinical and Experimental Medicine, University of Messina, Via Consolare Valeria 1, 98125 Messina, Italy. FAU - Martini, Maurizio AU - Martini M AUID- ORCID: 0000-0002-6260-6310 AD - Department of Human Pathology of Adults and Developmental Age "Gaetano Barresi", Division of Pathology, University of Messina, Via Consolare Valeria 1, 98125 Messina, Italy. FAU - Fadda, Guido AU - Fadda G AD - Department of Human Pathology of Adults and Developmental Age "Gaetano Barresi", Division of Pathology, University of Messina, Via Consolare Valeria 1, 98125 Messina, Italy. FAU - Tuccari, Giovanni AU - Tuccari G AUID- ORCID: 0000-0002-1561-4246 AD - Department of Human Pathology of Adults and Developmental Age "Gaetano Barresi", Division of Pathology, University of Messina, Via Consolare Valeria 1, 98125 Messina, Italy. FAU - Ieni, Antonio AU - Ieni A AD - Department of Human Pathology of Adults and Developmental Age "Gaetano Barresi", Division of Pathology, University of Messina, Via Consolare Valeria 1, 98125 Messina, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705810 OTO - NOTNLM OT - breast pathology OT - differential diagnosis OT - immunohistochemistry OT - molecular pathology OT - morphology OT - triple-negative breast carcinoma COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:40 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - jcm15208197 [pii] AID - jcm-15-08197 [pii] AID - 10.3390/jcm15208197 [doi] PST - epublish SO - J Clin Med. 2026 Oct 21;15(20):8197. doi: 10.3390/jcm15208197. PMID- 42941708 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Outcome-Specific Performance of HALP, AIMS65, Glasgow-Blatchford, and Rockall Scores in Emergency Department Patients with Acute Upper Gastrointestinal Bleeding: A Retrospective Cohort Study. LID - 10.3390/jcm15208196 [doi] LID - 8196 AB - Background/Objectives: The Hemoglobin-Albumin-Lymphocyte-Platelet (HALP) score integrates hematologic, nutritional, immune, and platelet parameters, but its prognostic value in acute upper gastrointestinal bleeding (UGIB) remains uncertain. We compared the performance of HALP with AIMS65, Glasgow-Blatchford Score (GBS), and Rockall scores. Methods: We retrospectively studied consecutive adults with endoscopy-confirmed UGIB presenting to the emergency department from January 2021 through December 2024. Outcomes were in-hospital mortality, packed red blood cell (PRBC) transfusion within 24 h, and composite severe UGIB, defined as >/=2 PRBC units transfused within 24 h or UGIB-related intensive care unit admission. Discrimination was assessed using receiver operating characteristic curves and areas under the curve (AUCs); pairwise comparisons used the correlated-sample DeLong test. Firth-penalized logistic regression assessed the adjusted association between HALP and in-hospital mortality. Results: Of 209 patients, 24 (11.5%) died and 36 (17.2%) had variceal bleeding. For mortality, AUCs were 0.580 for inverse HALP, 0.802 for AIMS65, 0.733 for GBS, and 0.731 for Rockall. For 24-h PRBC transfusion, AUCs were 0.629, 0.642, 0.814, and 0.511, respectively; for severe UGIB, they were 0.622, 0.594, 0.781, and 0.501. No statistically significant adjusted association between HALP and mortality was observed (aOR, 0.91 per 10-point increase; p = 0.571). Conclusions: HALP showed limited discrimination across the evaluated UGIB outcomes, and no statistically significant adjusted association with mortality was observed. AIMS65 had the highest observed AUC point estimate for mortality, although its AUC did not differ significantly from those of GBS or Rockall. GBS had the highest AUCs for PRBC transfusion and severe UGIB. These findings support outcome-specific selection of UGIB risk scores and do not support the use of HALP as a stand-alone risk-stratification tool without external validation. FAU - Barut, Esat AU - Barut E AUID- ORCID: 0000-0003-0844-0468 AD - Department of Emergency Medicine, Faculty of Medicine, Harran University, Sanliurfa 63300, Turkiye. FAU - Yasak, Ibrahim Halil AU - Yasak IH AUID- ORCID: 0000-0002-6399-7755 AD - Department of Emergency Medicine, Faculty of Medicine, Harran University, Sanliurfa 63300, Turkiye. FAU - Seyhanli, Eyyup Sabri AU - Seyhanli ES AD - Department of Emergency Medicine, Mehmet Akif Inan Training and Research Hospital, Sanliurfa 63300, Turkiye. FAU - Giden, Ramazan AU - Giden R AUID- ORCID: 0000-0003-2127-1056 AD - Department of Emergency Medicine, Faculty of Medicine, Harran University, Sanliurfa 63300, Turkiye. FAU - Barut, Kemal AU - Barut K AD - Department of Emergency Medicine, Gazi Yasargil Training and Research Hospital, Diyarbakir 21070, Turkiye. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705710 OTO - NOTNLM OT - AIMS65 OT - Glasgow-Blatchford OT - HALP OT - Rockall OT - emergency department OT - upper gastrointestinal bleeding COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:40 PHST- 2026/09/21 00:00 [received] PHST- 2026/10/19 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - jcm15208196 [pii] AID - jcm-15-08196 [pii] AID - 10.3390/jcm15208196 [doi] PST - epublish SO - J Clin Med. 2026 Oct 21;15(20):8196. doi: 10.3390/jcm15208196. PMID- 42941703 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - The Silent Face of Atrial Fibrillation: A Systematic Review of Associated Factors, Clinical Phenotype, and Outcomes. LID - 10.3390/jcm15208191 [doi] LID - 8191 AB - Background: Asymptomatic atrial fibrillation (AF) is increasingly recognized through incidental diagnosis and continuous rhythm monitoring, yet its clinical phenotype and prognostic significance remain incompletely characterized. We systematically evaluated factors associated with asymptomatic AF and its clinical outcomes. Methods: This systematic review was conducted according to PRISMA 2020 recommendations. PubMed/MEDLINE, Scopus, and EMBASE were searched from inception through 1 September 2026. Studies reporting demographic, clinical, anthropometric, laboratory, electrocardiographic, imaging, or therapeutic correlates of asymptomatic AF and/or associated clinical outcomes were eligible. Given substantial clinical and methodological heterogeneity, evidence was synthesized using structured qualitative analyses and descriptive summaries of study-level data, without formal meta-analysis or inferential testing of aggregated between-group differences. Results: Twenty-three studies were included, comprising 47,456 patients with asymptomatic or subclinical AF and 126,894 participants in the corresponding comparator groups. Study-specific evidence identified male sex, lower ventricular rate, and long-standing persistent or permanent AF as relevant correlates of asymptomatic presentation. Greater left atrial remodeling, including larger atrial dimensions and impaired mechanical function, was also reported in selected studies of silent AF, whereas conventional left ventricular structure and systolic function showed relatively similar descriptive estimates across the available datasets. Selected studies identified metabolic biomarkers and thoracic morphology as additional potential correlates. Rhythm-control strategies and catheter ablation showed numerically lower utilization in descriptive summaries of asymptomatic AF, although the contributing populations were heterogeneous and not necessarily directly comparable. Prognostic findings were heterogeneous: mortality and thromboembolic outcomes were generally comparable between asymptomatic and symptomatic AF in several individual studies, whereas other investigations reported a greater burden of heart failure and heart failure-related events among symptomatic patients. Conclusions: Asymptomatic AF represents a heterogeneous clinical phenotype rather than a benign form of the arrhythmia. A lower ventricular response may attenuate symptom perception, while atrial remodeling and cardiovascular risk may progress despite the absence of symptoms. Symptom status should therefore not replace established cardiovascular risk assessment. Thoracic geometry may represent an additional determinant of symptom perception, although current evidence remains preliminary and requires prospective validation. FAU - Sonaglioni, Andrea AU - Sonaglioni A AUID- ORCID: 0000-0001-7641-8831 AD - Division of Cardiology, IRCCS MultiMedica, 20123 Milan, Italy. FAU - Gramaglia, Giulio Francesco AU - Gramaglia GF AUID- ORCID: 0009-0005-7084-9442 AD - Department of Emergency, Fondazione IRCSS Ca' Granda, Ospedale Maggiore Policlinico, 20122 Milan, Italy. FAU - Mondazzi, Elena AU - Mondazzi E AD - Department of Emergency, Fondazione IRCSS Ca' Granda, Ospedale Maggiore Policlinico, 20122 Milan, Italy. FAU - Nicolosi, Gian Luigi AU - Nicolosi GL AUID- ORCID: 0000-0002-2218-2808 AD - Division of Cardiology, Policlinico San Giorgio, 33170 Pordenone, Italy. FAU - Lombardo, Michele AU - Lombardo M AD - Division of Cardiology, IRCCS MultiMedica, 20123 Milan, Italy. FAU - Baravelli, Massimo AU - Baravelli M AD - Division of Cardiology, IRCCS MultiMedica, 20123 Milan, Italy. LA - eng GR - CUP: I43C25000300001/Ministero della Salute/ PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706439 OTO - NOTNLM OT - asymptomatic atrial fibrillation OT - atrial fibrillation OT - atrial remodeling OT - cardiovascular outcomes OT - heart failure OT - silent atrial fibrillation OT - subclinical atrial fibrillation OT - symptom status OT - systematic review COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:40 PHST- 2026/09/13 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - jcm15208191 [pii] AID - jcm-15-08191 [pii] AID - 10.3390/jcm15208191 [doi] PST - epublish SO - J Clin Med. 2026 Oct 21;15(20):8191. doi: 10.3390/jcm15208191. PMID- 42941696 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - A Self-Care Mobile Application for Patients with Acute Decompensated Heart Failure During the COVID-19 Pandemic. LID - 10.3390/jcm15208183 [doi] LID - 8183 AB - Background: Mobile health applications may support self-care following acute decompensated heart failure (ADHF), particularly when conventional healthcare contact is limited. We evaluated the integration of the Aintree Heart Failure Passport (AHFP) application into a specialist ambulatory ADHF pathway and described self-care behaviour, medication adherence and 30-day heart failure rehospitalisation among app users. Methods: This prospective, single-centre observational study included consecutive patients treated with day-case intravenous diuretics in a consultant-led ambulatory heart failure unit at a UK university hospital between March 2020 and July 2021. Patients with access to a smartphone were offered the AHFP application. Self-care behaviour and medication adherence were assessed two weeks after presentation using the nine-item European Heart Failure Self-care Behaviour Scale (EHFScB-9) and five-item Medication Adherence Report Scale (MARS-5), respectively. Thirty-day HF hospitalisation among application users was compared with a separate cohort of hospitalised HF patients recorded in the National Heart Failure Audit (73/382, 19.1%). This inpatient cohort was used as a contextual benchmark rather than a matched control group. Results: Of 221 consecutive patients treated for ADHF, 148 (67%) downloaded and registered with the AHFP application. Their mean age was 62 +/- 6.1 years, and 45% were women. At two weeks, the mean EHFScB-9 score was 19.1 +/- 6.7, and the mean MARS-5 score was 23.3 +/- 1.0. Within 30 days, 16 patients (10.8%; 95% CI 6.8-16.8%) were hospitalised with HF, accounting for 73 inpatient bed-days. The corresponding 30-day readmission rate in the separate inpatient audit cohort was 19.1% (73/382). Conclusions: The AHFP application was downloaded and registered by 67% of patients treated within an ambulatory ADHF pathway. Self-care behaviour and medication adherence were assessed at two weeks. The observed 30-day HF hospitalisation rate was lower than that of a separate inpatient audit cohort, although differences in patient selection and care setting preclude attribution to the application. These findings support the feasibility of integrating a self-care application into specialist ambulatory HF care and warrant further evaluation in controlled studies. FAU - Essa, Hani AU - Essa H AUID- ORCID: 0000-0001-9445-2582 AD - Department of Cardiology, Aintree University Hospital, Liverpool University Hospitals NHS Foundation Trust, Lower Lane, Liverpool L9 7AL, UK. AD - Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool L7 8TX, UK. FAU - Lip, Gregory Yoke Hong AU - Lip GYH AUID- ORCID: 0000-0002-7566-1626 AD - Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool L7 8TX, UK. AD - Department of Clinical Medicine, Aalborg University, 9000 Aalborg, Denmark. AD - Department of Cardiology, Lipidology and Internal Medicine, Medical University of Bialystok, 15-540 Bialystok, Poland. FAU - Sankaranarayanan, Rajiv AU - Sankaranarayanan R AUID- ORCID: 0000-0003-2355-2011 AD - Department of Cardiology, Aintree University Hospital, Liverpool University Hospitals NHS Foundation Trust, Lower Lane, Liverpool L9 7AL, UK. AD - Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool L7 8TX, UK. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706181 OTO - NOTNLM OT - acute decompensated heart failure OT - ambulatory heart failure care OT - mobile health (mHealth) OT - self-care COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:40 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - jcm15208183 [pii] AID - jcm-15-08183 [pii] AID - 10.3390/jcm15208183 [doi] PST - epublish SO - J Clin Med. 2026 Oct 21;15(20):8183. doi: 10.3390/jcm15208183. PMID- 42941693 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Comparative Outcomes of Hip Fractures in Centenarians Compared to Non-Centenarians. LID - 10.3390/jcm15208180 [doi] LID - 8180 AB - Background/Objectives: The number of hip fractures among centenarians is rising, yet their outcomes and the factors influencing mortality remain inadequately characterized. This study compared postoperative outcomes between centenarians and non-centenarians and examined perioperative factors associated with mortality. Methods: This retrospective cohort study included 31 centenarians (>/=100 years) who underwent hip fracture surgery between October 2022 and March 2024 and 176 consecutive non-centenarians (65-99 years) admitted between October 2022 and April 2023. Associations between centenarian status, American Society of Anesthesiologists (ASA) score, comorbidity-only Charlson Comorbidity Index (CCI), surgical procedure, and time to surgery with 30-day and 12-month mortality were analyzed using logistic regression with clinically pre-selected covariates (Firth's method for 30-day mortality). Results: Centenarians exhibited higher ASA scores, while CCI values did not differ between groups. Centenarians experienced longer intensive care unit and hospital stays. The complication rate was numerically higher among centenarians (35.5% vs. 30.1%), although this difference was not statistically significant (p = 0.55). Thirty-day mortality was 29.0% for centenarians compared to 6.2% for non-centenarians, and 12-month mortality was 80.6% versus 30.1% (both p < 0.001). Centenarian status (30-day odds ratio [OR] 4.87, 95% confidence interval [CI] 1.73-13.60; 12-month OR 8.19, 95% CI 3.16-24.22) and higher ASA scores were associated with increased mortality, whereas CCI was not. Longer time to surgery was associated with higher 12-month mortality (OR 1.23 per 24 h, 95% CI 1.01-1.51). Conclusions: Centenarians demonstrated substantially higher short- and long-term mortality following hip fracture surgery compared to younger older adults. Extreme age and elevated ASA scores were associated with increased mortality, although unmeasured factors such as frailty may contribute to these associations. Hip fracture in centenarians should be considered a major life-limiting event that necessitates early goals-of-care discussions. FAU - Cevik, Huseyin Bilgehan AU - Cevik HB AUID- ORCID: 0000-0003-1945-3715 AD - Department of Orthopedics and Traumatology, Ankara Etlik City Hospital, University of Health Sciences, 06170 Ankara, Turkiye. FAU - Acar, Ahmet AU - Acar A AUID- ORCID: 0000-0001-8378-4346 AD - Department of Orthopedics and Traumatology, Ankara Etlik City Hospital, University of Health Sciences, 06170 Ankara, Turkiye. FAU - Girgin, Ahmet Berkay AU - Girgin AB AUID- ORCID: 0000-0003-3293-8231 AD - Department of Orthopedics and Traumatology, Akyurt State Hospital, 06750 Ankara, Turkiye. FAU - Aycicek, Gozde Sengul AU - Aycicek GS AUID- ORCID: 0000-0003-0528-8851 AD - Department of Geriatrics, Ankara Etlik City Hospital, University of Health Sciences, 06170 Ankara, Turkiye. FAU - Koca, Meltem AU - Koca M AD - Department of Geriatrics, Ankara Etlik City Hospital, University of Health Sciences, 06170 Ankara, Turkiye. FAU - Atli, Osman Yagiz AU - Atli OY AUID- ORCID: 0000-0001-6657-6860 AD - Department of Orthopedics and Traumatology, Ankara Etlik City Hospital, University of Health Sciences, 06170 Ankara, Turkiye. FAU - Torun, Omer AU - Torun O AUID- ORCID: 0000-0002-5787-3908 AD - Department of Orthopedics and Traumatology, Ankara Etlik City Hospital, University of Health Sciences, 06170 Ankara, Turkiye. FAU - Duman, Evrim AU - Duman E AUID- ORCID: 0000-0002-3493-5125 AD - Department of Orthopedics and Traumatology, Ankara Etlik City Hospital, University of Health Sciences, 06170 Ankara, Turkiye. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705695 OTO - NOTNLM OT - ASA score OT - centenarians OT - hip fracture OT - mortality OT - survival COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:40 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - jcm15208180 [pii] AID - jcm-15-08180 [pii] AID - 10.3390/jcm15208180 [doi] PST - epublish SO - J Clin Med. 2026 Oct 21;15(20):8180. doi: 10.3390/jcm15208180. PMID- 42941690 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Neuropelveology in Endometriosis: Pain Mechanisms, Diagnostic Implications, and Surgical Decision-Making. LID - 10.3390/jcm15208177 [doi] LID - 8177 AB - Endometriosis is a systemic, estrogen dependent neuroinflammatory-mediated disease affecting approximately 10-15% of women of reproductive age. Clinical manifestations include chronic pelvic pain (CPP), dysmenorrhea, dyspareunia, dyschezia, dysuria, and infertility. Endometriosis-associated pain is multifactorial and comprises overlapping nociceptive, neuropathic, and nociplastic pain phenotypes driven by persistent inflammation, neuroangiogenesis, peripheral nerve involvement, and central sensitization (CS). These mechanisms may sustain pain beyond lesion excision, highlighting the importance of pelvic nervous system (PNS) involvement. This narrative review examines the role of neuropelveology (NP) in endometriosis, focusing on pain mechanisms, diagnostic implications, and surgical decision making. NP integrates pelvic neuroanatomy and neurophysiology into structured neurological assessment, pain mapping, symptom localization, and nerve sparing surgery. Current evidence suggests that NP may complement conventional evaluation in selected patients with refractory pain, neuropathic symptoms, discordance between symptom severity and imaging findings, or suspected involvement of the sacral plexus or sciatic nerve. Recognizing distinct pain phenotypes may support individualized treatment planning by balancing lesion excision with neural preservation and identifying patients who may benefit from multidisciplinary pain management, rehabilitation, neurolysis, or neuromodulation. Although current evidence remains limited, NP should be considered a complementary framework rather than a replacement for established endometriosis management. Prospective multicenter studies using standardized diagnostic criteria and long-term functional outcomes are needed to define its clinical utility. FAU - Gabriela, Cano-Herrera AU - Gabriela CH AUID- ORCID: 0009-0006-1334-0399 AD - Escuela de Ciencias de la Salud, Universidad Anahuac Puebla, Puebla 72810, Mexico. FAU - Loza Lopez, Ericka Cristina AU - Loza Lopez EC AUID- ORCID: 0000-0003-2839-0963 AD - Centro de Investigacion en Ciencias de la Salud (CICSA), Facultad de Ciencias de la Salud Universidad Anahuac Mexico Norte, Huixquilucan 52786, Mexico. FAU - Bautista Gonzalez, Maria Fernanda AU - Bautista Gonzalez MF AD - Centro de Investigacion en Ciencias de la Salud (CICSA), Facultad de Ciencias de la Salud Universidad Anahuac Mexico Norte, Huixquilucan 52786, Mexico. FAU - Esparza Salazar, Felipe AU - Esparza Salazar F AUID- ORCID: 0000-0003-1884-5389 AD - Centro de Investigacion en Ciencias de la Salud (CICSA), Facultad de Ciencias de la Salud Universidad Anahuac Mexico Norte, Huixquilucan 52786, Mexico. AD - Department of Neurosurgery and Brain Repair, Center of Excellence for Aging and Brain Repair, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA. FAU - Mena Guerrero, Tamara AU - Mena Guerrero T AUID- ORCID: 0009-0004-1851-6774 AD - Centro de Investigacion en Ciencias de la Salud (CICSA), Facultad de Ciencias de la Salud Universidad Anahuac Mexico Norte, Huixquilucan 52786, Mexico. FAU - De Leon Vela, Diego AU - De Leon Vela D AUID- ORCID: 0009-0004-7282-9472 AD - Centro de Investigacion en Ciencias de la Salud (CICSA), Facultad de Ciencias de la Salud Universidad Anahuac Mexico Norte, Huixquilucan 52786, Mexico. FAU - van Tienhoven, Ximena A AU - van Tienhoven XA AUID- ORCID: 0000-0003-1127-9548 AD - Centro de Investigacion en Ciencias de la Salud (CICSA), Facultad de Ciencias de la Salud Universidad Anahuac Mexico Norte, Huixquilucan 52786, Mexico. FAU - Simental Aldaba, Emmanuel AU - Simental Aldaba E AUID- ORCID: 0000-0001-8504-8457 AD - Centro de Rehabilitacion e Inclusion Infantil Teleton Estado de Mexico, Tlalnepantla 54010, Mexico. FAU - Massieu Perez, Patricia Carolina AU - Massieu Perez PC AD - Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Ciudad de Mexico 04510, Mexico. FAU - Mena Garcia, Layra AU - Mena Garcia L AUID- ORCID: 0009-0005-3378-4897 AD - Facultad de Ciencias de la Salud, Universidad Panamericana, Ciudad de Mexico 03920, Mexico. FAU - Rivera Rosas, Eder Gabriel AU - Rivera Rosas EG AUID- ORCID: 0009-0000-9778-6959 AD - Female Pelvic Surgery Department, Doyenne High Quality and Multidisciplinary Treatment Center for Endometriosis, Ciudad de Mexico 06700, Mexico. FAU - Carbajal Ocampo, Dante AU - Carbajal Ocampo D AD - Female Pelvic Surgery Department, Doyenne High Quality and Multidisciplinary Treatment Center for Endometriosis, Ciudad de Mexico 06700, Mexico. FAU - Cabrera Carranco, Ramiro AU - Cabrera Carranco R AUID- ORCID: 0000-0003-3823-3283 AD - Female Pelvic Surgery Department, Doyenne High Quality and Multidisciplinary Treatment Center for Endometriosis, Ciudad de Mexico 06700, Mexico. FAU - Vera Hernandez, Perla Sofia AU - Vera Hernandez PS AUID- ORCID: 0009-0009-9335-7503 AD - Female Pelvic Surgery Department, Doyenne High Quality and Multidisciplinary Treatment Center for Endometriosis, Ciudad de Mexico 06700, Mexico. FAU - Garcia Gaona, Axel Ranferi AU - Garcia Gaona AR AUID- ORCID: 0009-0001-0866-4819 AD - Female Pelvic Surgery Department, Doyenne High Quality and Multidisciplinary Treatment Center for Endometriosis, Ciudad de Mexico 06700, Mexico. FAU - Cueto Camara, Jonathan Fidel AU - Cueto Camara JF AD - Female Pelvic Surgery Department, Doyenne High Quality and Multidisciplinary Treatment Center for Endometriosis, Ciudad de Mexico 06700, Mexico. FAU - Armenta Moreno, Javier Ivan AU - Armenta Moreno JI AD - Centro de Investigacion en Ciencias de la Salud (CICSA), Facultad de Ciencias de la Salud Universidad Anahuac Mexico Norte, Huixquilucan 52786, Mexico. FAU - Sierra Brozon, Ana Gabriela AU - Sierra Brozon AG AUID- ORCID: 0009-0002-1898-9577 AD - Female Pelvic Surgery Department, Doyenne High Quality and Multidisciplinary Treatment Center for Endometriosis, Ciudad de Mexico 06700, Mexico. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705954 OTO - NOTNLM OT - chronic pelvic pain OT - endometriosis OT - neuroinflammation OT - neuropelveology OT - pelvic nervous system COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:40 PHST- 2026/08/18 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - jcm15208177 [pii] AID - jcm-15-08177 [pii] AID - 10.3390/jcm15208177 [doi] PST - epublish SO - J Clin Med. 2026 Oct 21;15(20):8177. doi: 10.3390/jcm15208177. PMID- 42941689 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Initial Clip Selection and Outcomes After Mitral Transcatheter Edge-to-Edge Repair: A Single-Center Experience. LID - 10.3390/jcm15208176 [doi] LID - 8176 AB - Background: Whether the initial clip type selected during transcatheter edge-to-edge repair (TEER) of the mitral valve-grasping width and arm length-independently influences procedural and echocardiographic outcomes remains uncertain. Methods: We retrospectively analyzed 138 consecutive patients undergoing mitral TEER. The first implanted clip was classified as narrow standard-length (NT), long narrow-arm (XT), or long wide-arm (XTW). Determinants of initial clip selection and its association with procedural success (residual mitral regurgitation [MR] /=2 clips were assessed using multivariable logistic and linear regression. Results: Median age was 74.0 (66.0-80.0), 74.5 (66.0-82.5), and 75.0 (70.0-79.0) years, and median LVEF was 35.0% (30.0-50.0), 30.0% (25.0-45.0), and 40.0% (25.0-45.0), for the NT, XT, and XTW groups, respectively; 66% of patients overall were male. An XTW clip was implanted first in 61.6% of patients. XTW selection was independently associated with larger effective regurgitant orifice area, lower LVEF, longer posterior leaflet length, and secondary MR (all p < 0.05), whereas NT selection favored younger age, smaller orifice, and higher LVEF. After adjustment for these factors, no statistically significant independent association was detected between initial clip type and residual MR >/=2+, post-procedural transmitral gradient, or the need for additional clips (all p > 0.05); however, confidence intervals were wide. On univariable analysis, larger left atrial size and moderate-to-severe tricuspid regurgitation were associated with residual MR >/= 2+, but neither remained significant after multivariable adjustment. Conclusions: Initial clip selection was associated with valve anatomy, LVEF and MR etiology. No statistically significant independent association between initial clip type and procedural or short-term echocardiographic outcomes was detected in this cohort. Given the observational design, confounding by indication, limited sample size and wide confidence intervals, clinically relevant effects cannot be excluded, and these findings do not establish the superiority or equivalence of any clip-selection strategy. FAU - Akhundova, Aysel AU - Akhundova A AD - Department of Cardiology, Medipol Mega University Hospital, Istanbul Medipol University, 34214 Istanbul, Turkey. FAU - Keskin, Berhan AU - Keskin B AD - Department of Cardiology, Medipol Mega University Hospital, Istanbul Medipol University, 34214 Istanbul, Turkey. FAU - Savur, Umeyir AU - Savur U AUID- ORCID: 0000-0003-1320-9033 AD - Department of Cardiology, Medipol Mega University Hospital, Istanbul Medipol University, 34214 Istanbul, Turkey. FAU - Onder, Ozlem AU - Onder O AUID- ORCID: 0009-0004-5704-2145 AD - Department of Cardiology, Medipol Mega University Hospital, Istanbul Medipol University, 34214 Istanbul, Turkey. FAU - Yigitbay, Ebubekir AU - Yigitbay E AD - Department of Cardiology, Medipol Mega University Hospital, Istanbul Medipol University, 34214 Istanbul, Turkey. FAU - Guler, Ekrem AU - Guler E AD - Department of Cardiology, Medipol Mega University Hospital, Istanbul Medipol University, 34214 Istanbul, Turkey. FAU - Gunes, Haci Murat AU - Gunes HM AD - Department of Cardiology, Medipol Mega University Hospital, Istanbul Medipol University, 34214 Istanbul, Turkey. FAU - Arman, Mehmet Emir AU - Arman ME AUID- ORCID: 0000-0002-4045-4969 AD - Internal Medicine, Ascension St Vincent Hospital-Indianapolis, Indianapolis, IN 46260, USA. FAU - Boztosun, Bilal AU - Boztosun B AD - Department of Cardiology, Medipol Mega University Hospital, Istanbul Medipol University, 34214 Istanbul, Turkey. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705671 OTO - NOTNLM OT - MitraClip OT - clip selection OT - mitral regurgitation OT - mitral valve OT - residual mitral regurgitation OT - transcatheter edge-to-edge repair OT - transmitral gradient COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:40 PHST- 2026/09/12 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - jcm15208176 [pii] AID - jcm-15-08176 [pii] AID - 10.3390/jcm15208176 [doi] PST - epublish SO - J Clin Med. 2026 Oct 21;15(20):8176. doi: 10.3390/jcm15208176. PMID- 42941687 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Total Corneal Aberration Measurements by Anterior Segment Optical Coherence Tomography and Scheimpflug-Based Imaging: A Narrative Review of Optical Principles and Sources of Inter-Device Discordance. LID - 10.3390/jcm15208174 [doi] LID - 8174 AB - Total corneal aberration analysis is increasingly used as part of intraocular lens selection, ectasia screening, and refractive surgery planning. Scheimpflug-based tomography and anterior segment optical coherence tomography (AS-OCT) are commonly used imaging techniques to identify the anterior and posterior corneal surfaces; however, many devices combine these methods with Placido-disc topography or reconstruction algorithms. Although these devices provide anterior, posterior, and total corneal wavefront aberrations, multiple studies have shown that Zernike coefficients obtained with different systems are not consistently interchangeable, particularly for posterior-surface-derived and higher-order terms such as trefoil and total higher-order aberration root-mean-square values. This narrative review summarizes the optical principles of Scheimpflug imaging and AS-OCT, synthesizes the available comparative literature, and analyzes the mechanisms that may contribute to inter-device discordance, including differences in light sources and light-tissue interactions, posterior-surface reconstruction and refractive-index assumptions, edge-detection and Zernike-fitting algorithms, sampling geometry and acquisition time, and reference-axis selection. We discuss the clinical implications of these discrepancies and propose a research agenda, including model-eye validation and ray-tracing simulation studies, to help distinguish the effects of hardware optics from those of proprietary reconstruction algorithms. FAU - Kim, Doyeon AU - Kim D AUID- ORCID: 0009-0005-9263-8646 AD - Department of Medicine, Keimyung University School of Medicine, Daegu 42601, Republic of Korea. FAU - Bang, Seung Pil AU - Bang SP AUID- ORCID: 0000-0002-7521-544X AD - Department of Ophthalmology, Keimyung University School of Medicine, Daegu 42601, Republic of Korea. LA - eng GR - IRIS RS-2024-00457444/National Research Foundation of Korea/ PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706273 OTO - NOTNLM OT - Scheimpflug imaging OT - anterior segment optical coherence tomography OT - corneal tomography OT - higher-order aberration OT - intraocular lens calculation OT - total corneal aberration OT - wavefront analysis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:40 PHST- 2026/09/26 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - jcm15208174 [pii] AID - jcm-15-08174 [pii] AID - 10.3390/jcm15208174 [doi] PST - epublish SO - J Clin Med. 2026 Oct 21;15(20):8174. doi: 10.3390/jcm15208174. PMID- 42941686 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Chin Ptosis: Etiology, Classification, Diagnosis and Surgical Management-A Literature Review. LID - 10.3390/jcm15208175 [doi] LID - 8175 AB - Background/Objectives: Chin ptosis is a heterogeneous deformity characterized by inferior displacement of the presymphyseal soft tissues and may reflect abnormalities of skeletal support, the mentalis muscle, the submental crease, the soft-tissue envelope, or facial animation. This narrative review aimed to synthesize the anatomy, etiology, classification, diagnostic assessment, and the management of chin ptosis and to translate the available evidence into a component-based clinical framework. Methods: MEDLINE via PubMed was searched from inception to 6 October 2026 and yielded 62 records. Targeted Google Scholar searches and backward citation searching identified 22 additional unique sources not retrieved by the PubMed strategy. Forty-two publications were included. Anatomical studies, technical reports, clinical series, comparative studies, reviews, and relevant book chapters were evaluated. Evidence was classified as direct when it concerned diagnosed chin ptosis and as indirect when it concerned related conditions such as chin retrusion, macrogenia, facial aging, or mentalis hyperactivity. Results: The four-category classification of illusory, developmental, iatrogenic, and dynamic chin ptosis derives from the framework of Garfein and Zide and was retained with additional clarification. The categories are not mutually exclusive. Diagnosis should integrate history, examination at rest and during animation, skeletal and dental assessment, standardized photography or video, reproducible soft-tissue measurements, and patient-reported outcomes. Structural mentalis detachment generally requires anatomical reattachment, whereas crease abnormalities, skeletal mismatch, redundant soft tissue, or generalized cervicofacial laxity require component-specific correction. Nonsurgical treatments mainly address projection deficiency or muscular hyperactivity and should not be presented as definitive treatment for true structural ptosis. Conclusions: Mechanism-based diagnosis and treatment selection are essential. Current evidence remains dominated by technical reports and small retrospective series; standardized diagnostic criteria, complication reporting, and comparative prospective studies are needed. FAU - Rolek, Agnieszka AU - Rolek A AUID- ORCID: 0009-0007-2880-2055 AD - Independent Researcher, ul. R. Dmowskiego 1A/43, 50-203 Wroclaw, Poland. FAU - Pokrowiecki, Rafal AU - Pokrowiecki R AUID- ORCID: 0000-0001-8604-1468 AD - Independent Researcher, Woronicza 31/33, 02-640 Warsaw, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706216 OTO - NOTNLM OT - chin ptosis OT - chin rejuvenation OT - facial aging OT - genioplasty OT - mentalis muscle OT - witch's chin COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:40 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - jcm15208175 [pii] AID - jcm-15-08175 [pii] AID - 10.3390/jcm15208175 [doi] PST - epublish SO - J Clin Med. 2026 Oct 21;15(20):8175. doi: 10.3390/jcm15208175. PMID- 42941681 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Channel-Focused Modified TURP for Post-Radiation Bladder Outlet Obstruction: A Case Series. LID - 10.3390/jcm15208168 [doi] LID - 8168 AB - Background/Objectives: Bladder outlet obstruction (BOO) after prostate radiotherapy is surgically challenging because of radiation-induced tissue changes and functional complications. We evaluated the efficacy and safety of channel-focused modified transurethral resection of the prostate (RadTURP) for post-radiation BOO. Methods: This retrospective case series included 11 patients who underwent RadTURP for post-radiation BOO between 2014 and 2025. Outcomes included symptom scores, voiding parameters, and postoperative complications. Pre-post changes were assessed using the Wilcoxon signed-rank test. Results: Median age was 75 years and median follow-up was 29 months. The median resection proportion was 24%. IPSS improved from 27 to 10 (p = 0.003), QoL from 5 to 3 (p = 0.007), OABSS from 12 to 6 (p = 0.016), Qmax from 6 to 17 mL/s (p = 0.006), and PVR from 59 to 0 mL (p = 0.047). De novo urinary incontinence occurred in four patients (36.4%), including two (18.2%) with persistent stress urinary incontinence requiring artificial urinary sphincter implantation. Among six patients undergoing symptom-driven postoperative cystoscopy, five (83.3%) had stone/calcification-related findings requiring energy-free endoscopic removal. One patient (9.1%) developed a urethral stricture requiring repeated dilation. Conclusions: In this small case series, channel-focused RadTURP was associated with improved urinary symptoms and voiding function after prostate radiotherapy. However, de novo urinary incontinence occurred in 36.4% of patients, highlighting an important postoperative safety concern for patient counseling and selection. Larger studies are needed to define durability and safety. FAU - Lee, Jeong Hyun AU - Lee JH AUID- ORCID: 0009-0008-1813-9736 AD - Department of Urology, Severance Hospital, College of Medicine, Yonsei University, Seoul 03722, Republic of Korea. FAU - Kim, Jang Hwan AU - Kim JH AD - Department of Urology, Severance Hospital, College of Medicine, Yonsei University, Seoul 03722, Republic of Korea. FAU - Oh, Kyung Tak AU - Oh KT AUID- ORCID: 0000-0003-3681-6629 AD - Department of Urology, Severance Hospital, College of Medicine, Yonsei University, Seoul 03722, Republic of Korea. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706472 OTO - NOTNLM OT - lower urinary tract symptoms OT - modified transurethral resection of the prostate OT - post-radiation bladder outlet obstruction OT - prostate radiotherapy complications OT - urinary incontinence outcomes COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:40 PHST- 2026/08/16 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - jcm15208168 [pii] AID - jcm-15-08168 [pii] AID - 10.3390/jcm15208168 [doi] PST - epublish SO - J Clin Med. 2026 Oct 20;15(20):8168. doi: 10.3390/jcm15208168. PMID- 42941680 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Takotsubo Syndrome in Solid Organ Transplant Recipients: Current Evidence, Clinical Challenges, and Future Perspectives-A Narrative Review. LID - 10.3390/jcm15208167 [doi] LID - 8167 AB - Takotsubo syndrome (TTS) is an acute and usually reversible form of myocardial dysfunction associated with emotional or physical stress. Solid organ transplantation (SOT) combines several potential triggers, including major surgical stress, hemodynamic instability, systemic inflammation, and exposure to exogenous catecholamines. Respiratory failure, calcineurin inhibitor toxicity, and altered cardiac innervation may also contribute. This narrative review summarizes current evidence on organ-specific timing, potential triggers, diagnostic challenges, management, and outcomes of TTS in SOT recipients. Reported cases predominantly occur early after liver and kidney transplantation, whereas presentations after heart transplantation range from the immediate postoperative period to years later. After heart transplantation, distinguishing TTS from early graft dysfunction and considering donor-side TTS are particularly important. Diagnostic certainty varies among published cases because formal criteria and the exclusion of competing diagnoses are inconsistently documented. Management largely follows recommendations for the general TTS population, with temporary mechanical circulatory support used in selected severe cases. Although ventricular function usually recovers, cardiogenic shock, arrhythmias, and multiorgan dysfunction may complicate the acute course, and persistent ventricular impairment has been associated with poorer long-term outcomes after liver transplantation. Comparisons with the general TTS population suggest a high proportion of severe presentations among reported transplant-associated cases; however, publication and ascertainment bias preclude firm conclusions about differences in incidence or severity. Evidence remains dominated by case reports, small series, and retrospective studies, underscoring the need for standardized reporting and prospective multicenter research. FAU - Kosek-Nikolajczuk, Malgorzata AU - Kosek-Nikolajczuk M AD - 1st Department of Cardiology, Medical University of Warsaw, Stefana Banacha 1a, 02-097 Warsaw, Poland. FAU - Piatkowski, Radoslaw AU - Piatkowski R AUID- ORCID: 0000-0003-1344-3348 AD - 1st Department of Cardiology, Medical University of Warsaw, Stefana Banacha 1a, 02-097 Warsaw, Poland. FAU - Grabowski, Marcin AU - Grabowski M AD - 1st Department of Cardiology, Medical University of Warsaw, Stefana Banacha 1a, 02-097 Warsaw, Poland. FAU - Budnik, Monika AU - Budnik M AUID- ORCID: 0000-0002-2982-3508 AD - 1st Department of Cardiology, Medical University of Warsaw, Stefana Banacha 1a, 02-097 Warsaw, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261020 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705804 OTO - NOTNLM OT - Takotsubo syndrome OT - cardiogenic shock OT - catecholamines OT - liver transplantation OT - mechanical circulatory support OT - solid organ transplantation OT - stress cardiomyopathy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:40 PHST- 2026/08/18 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - jcm15208167 [pii] AID - jcm-15-08167 [pii] AID - 10.3390/jcm15208167 [doi] PST - epublish SO - J Clin Med. 2026 Oct 20;15(20):8167. doi: 10.3390/jcm15208167. PMID- 42941676 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Differences in Pulp Wound Healing and Regenerative Potential Between SHEDs and DPSCs. LID - 10.3390/jcm15208162 [doi] LID - 8162 AB - Several challenges limit effective pulp wound healing during vital pulp therapies (VPTs) and pulp regeneration in necrotic teeth. Recent advances in regenerative therapies using dental mesenchymal stem cells (MSCs) have shown promise for improving tissue repair and expanding minimally invasive treatment strategies. Among these cells, stem cells from human exfoliated deciduous teeth (SHEDs) and dental pulp stem cells (DPSCs) have gained attention for cell-based VPTs (CB-VPTs) and cell-based regenerative endodontic procedures (CB-REPs). However, little is known about whether SHEDs or DPSCs perform better in CB-VPTs and CB-REPs, particularly regarding wound healing and dentin-pulp regeneration. To date, few studies have compared SHED and DPSC outcomes; separately, CB-VPTs and CB-REPs have shown promising outcomes with both cell types. Dentin bridge formation was generally observed following cell-free approaches, whereas recent in vivo studies of CB-VPTs and CB-REPs have additionally demonstrated the formation of pulp-like tissue. Histological analysis in animal models has identified odontoblasts, angiogenesis, neurogenesis, and connective tissue following CB-REPs using SHEDs or DPSCs. In humans, histological evidence of pulp-like tissue regeneration after SHED CB-REPs has been reported in a single patient. Collectively, preclinical findings and limited clinical evidence suggested that CB-REPs may support injury resolution accompanied by histological regeneration of pulp-like tissue. Both SHEDs and DPSCs represent promising candidates for CB-VPTs and CB-REPs; however, further comparative studies and randomized clinical trials are needed to strengthen the evidence on clinical effectiveness. This review examines the biological properties and regenerative potential of SHEDs and DPSCs within the dentin-pulp complex. FAU - Baldeon-Gutierrez, Rosa AU - Baldeon-Gutierrez R AUID- ORCID: 0000-0003-2283-7170 AD - Department of Pediatric Dentistry, Asahi University School of Dentistry, Gifu 501-0296, Japan. FAU - Terajima, Masahiko AU - Terajima M AD - Department of Anatomy, Asahi University School of Dentistry, Gifu 501-0296, Japan. FAU - Hori, Yuria AU - Hori Y AD - Department of Pediatric Dentistry, Asahi University School of Dentistry, Gifu 501-0296, Japan. FAU - Bando, Ryo AU - Bando R AD - Department of Pediatric Dentistry, Asahi University School of Dentistry, Gifu 501-0296, Japan. FAU - Kiyokawa, Yuki AU - Kiyokawa Y AUID- ORCID: 0000-0001-8406-130X AD - Department of Pediatric Dentistry, Asahi University School of Dentistry, Gifu 501-0296, Japan. FAU - Inada, Emi AU - Inada E AD - Department of Pediatric Dentistry, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan. FAU - Iwase, Yoko AU - Iwase Y AUID- ORCID: 0000-0002-5477-8307 AD - Department of Dentistry for the Disability and Oral Health, Asahi University School of Dentistry, Gifu 501-0296, Japan. FAU - Murakami, Tomoya AU - Murakami T AUID- ORCID: 0000-0002-1016-4960 AD - Kyoto Dental Service Center Central Clinic, Kyoto 604-8418, Japan. FAU - Watanabe, Satoshi AU - Watanabe S AUID- ORCID: 0009-0002-6252-5155 AD - Institute of Livestock and Grassland Science, Tsukuba 305-0901, Japan. FAU - Kubota, Naoko AU - Kubota N AD - Department of Pediatric Dentistry, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8544, Japan. FAU - Noguchi, Hirofumi AU - Noguchi H AUID- ORCID: 0000-0002-0880-6805 AD - Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa 903-0215, Japan. FAU - Sato, Masahiro AU - Sato M AUID- ORCID: 0000-0003-1334-2950 AD - Department of Genome Medicine, National Center for Child Health and Development, Tokyo 157-8535, Japan. FAU - Saitoh, Issei AU - Saitoh I AUID- ORCID: 0000-0002-7451-0666 AD - Department of Pediatric Dentistry, Asahi University School of Dentistry, Gifu 501-0296, Japan. LA - eng GR - 22H03277/Ministry of Education, Culture, Sports, Science and Technology/ GR - 25K0283/Ministry of Education, Culture, Sports, Science and Technology/ GR - 26K12934/Ministry of Education, Culture, Sports, Science and Technology/ PT - Journal Article PT - Review DEP - 20261020 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706074 OTO - NOTNLM OT - dental pulp stem cells (DPSCs) OT - dentin-pulp complex OT - dentin-pulp regeneration OT - pulp wound healing OT - regenerative endodontic procedures (REPs) OT - stem cells from human exfoliated deciduous teeth (SHEDs) OT - vital pulp therapies (VPTs) COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:40 PHST- 2026/09/12 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - jcm15208162 [pii] AID - jcm-15-08162 [pii] AID - 10.3390/jcm15208162 [doi] PST - epublish SO - J Clin Med. 2026 Oct 20;15(20):8162. doi: 10.3390/jcm15208162. PMID- 42941673 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Early Relative eGFR Decline After SGLT2 Inhibitor Initiation in Heart Failure: Clinical Correlates and Pretreatment-Anchored 12-Month Trajectories. LID - 10.3390/jcm15208158 [doi] LID - 8158 AB - Background/Objectives: Early changes in estimated glomerular filtration rate (eGFR) after sodium-glucose cotransporter 2 (SGLT2) inhibitor initiation in heart failure (HF) are difficult to interpret in routine practice. We assessed the clinical correlates of >10% early relative eGFR decline and the subsequent trajectories relative to the pretreatment eGFR. Methods: This retrospective single-center study included 295 patients with HF who remained on SGLT2 inhibitor treatment at their first eligible creatinine assessment on days 10-45 (median 25 days [IQR 21-30]; range 10-42). Multivariable regression, mixed-effects modeling, and within-patient paired analyses were used. Results: Among the 333 initiation episodes, 33 (9.9%) were excluded after early treatment discontinuation, and five repeat episodes were removed. Early decline >10% occurred in 110/295 patients (37.3%). Age >/= 80 years (adjusted OR 2.14, 95% CI 1.21-3.81), female sex (2.51, 1.42-4.44), higher baseline eGFR per 10 mL/min/1.73 m(2) (1.43, 1.21-1.69), and loop-diuretic use (1.82, 1.03-3.23) were associated with decline; the loop-diuretic association attenuated in an expanded model. Twelve-month eGFR was available for 195/295 patients (66.1%). Among the 71 with >10% early decline and paired 12-month data, the mean eGFR increased by 4.70 mL/min/1.73 m(2) from the early assessment but remained 6.99 mL/min/1.73 m(2) below the pretreatment (both p < 0.001). Conclusions: In this selected cohort, a mean increase after early decline did not indicate a return to the pretreatment mean. The interpretation of an isolated early eGFR value requires clinical context and subsequent measurements. FAU - Suzuki, Yoriyasu AU - Suzuki Y AUID- ORCID: 0000-0001-7979-9393 AD - Department of Cardiology, Aichi Medical University, Nagakute 480-1195, Aichi, Japan. AD - Department of Cardiology, Nagoya Heart Center, Nagoya 461-0045, Aichi, Japan. FAU - Onishi, Tomohiro AU - Onishi T AD - Department of Cardiology, Aichi Medical University, Nagakute 480-1195, Aichi, Japan. FAU - Nakano, Yusuke AU - Nakano Y AUID- ORCID: 0000-0002-9314-3427 AD - Department of Cardiology, Aichi Medical University, Nagakute 480-1195, Aichi, Japan. FAU - Shimoda, Masahiro AU - Shimoda M AD - Department of Cardiology, Aichi Medical University, Nagakute 480-1195, Aichi, Japan. FAU - Goto, Reiji AU - Goto R AD - Department of Cardiology, Aichi Medical University, Nagakute 480-1195, Aichi, Japan. FAU - Suzuki, Takahiko AU - Suzuki T AD - Department of Cardiology, Toyohashi Heart Center, Toyohashi 441-8530, Aichi, Japan. FAU - Amano, Tetsuya AU - Amano T AD - Department of Cardiology, Aichi Medical University, Nagakute 480-1195, Aichi, Japan. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706587 OTO - NOTNLM OT - SGLT2 inhibitor OT - cardiorenal assessment OT - estimated glomerular filtration rate OT - heart failure OT - kidney function COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:40 PHST- 2026/09/19 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:40 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - jcm15208158 [pii] AID - jcm-15-08158 [pii] AID - 10.3390/jcm15208158 [doi] PST - epublish SO - J Clin Med. 2026 Oct 20;15(20):8158. doi: 10.3390/jcm15208158. PMID- 42941669 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Diagnostic Value of the PEI-Q and Pancreatic Ultrasound for Identifying Reduced Pancreatic Exocrine Function in Early Type 2 Diabetes. LID - 10.3390/jcm15208157 [doi] LID - 8157 AB - Background/Objectives: Pancreatic symptoms and structural abnormalities do not necessarily correspond to reduced exocrine function in type 2 diabetes mellitus (T2DM). We aimed to estimate the frequency of reduced fecal elastase-1 (FE-1) and examine its associations with gastrointestinal symptom burden and pancreatic ultrasound findings. Methods: This cross-sectional study included 251 Kazakh adults with T2DM of 175-350 mL/h) initial rate; the primary outcome was escalation to advanced respiratory support or death within 12 h. Overlap weighting estimated marginal risk differences in the overlap population, with 2000 bootstrap resamples refitting the propensity-score model. Sensitivity and continuous dose-response analyses were pre-specified. Results: The primary outcome was classifiable in 2671 patients, with 143 events. Weighted 12 h risks were 4.95% and 6.60%: risk difference 1.65 percentage points (95% CI -0.41 to 3.74), risk ratio 1.33 (0.93 to 1.92). Invasive mechanical ventilation or death, a nested key secondary outcome unadjusted for multiplicity, gave 2.55 percentage points (0.71 to 4.59); 28-day mortality gave -1.45 (-4.10 to 1.30). In the pre-specified dose-response analysis, standardised 12 h risk was lowest near 140 mL/h (4.27%) and rose to 8.83% (6.56 to 11.28) at 350 mL/h. Conclusions: The primary analysis did not distinguish the two initial-rate strategies, although the interval remained compatible with a clinically important increase in risk. The secondary and dose-response findings are hypothesis-generating and do not support changing current infusion-rate practice. These estimates support the design and sample-size planning of a prospective trial assigning infusion rate. FAU - Zhao, Zhenkun AU - Zhao Z AD - Department of Emergency Medicine, West China Hospital, Sichuan University, Chengdu 610041, China. FAU - Chen, Xiaoye AU - Chen X AD - Department of General Surgery, West China Tianfu Hospital, Sichuan University, Chengdu 610041, China. FAU - Pan, Zhongjing AU - Pan Z AD - Department of Emergency Medicine, West China Hospital, Sichuan University, Chengdu 610041, China. FAU - Sha, Ao AU - Sha A AD - Department of Emergency Medicine, West China Hospital, Sichuan University, Chengdu 610041, China. FAU - Zhou, Yadong AU - Zhou Y AD - Department of Emergency Medicine, West China Hospital, Sichuan University, Chengdu 610041, China. FAU - Chen, Xiaoli AU - Chen X AD - Department of Emergency Medicine, West China Hospital, Sichuan University, Chengdu 610041, China. AD - West China School of Nursing, West China Hospital, Sichuan University, Chengdu 610041, China. FAU - Cheng, Tao AU - Cheng T AUID- ORCID: 0000-0003-1095-6439 AD - Department of Emergency Medicine, West China Hospital, Sichuan University, Chengdu 610041, China. FAU - Gan, Lu AU - Gan L AUID- ORCID: 0000-0001-8665-2760 AD - Department of Emergency Medicine, West China Hospital, Sichuan University, Chengdu 610041, China. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705826 OTO - NOTNLM OT - critical care OT - infusion rate OT - red blood cell transfusion OT - respiratory support OT - target trial emulation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:39 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - jcm15208147 [pii] AID - jcm-15-08147 [pii] AID - 10.3390/jcm15208147 [doi] PST - epublish SO - J Clin Med. 2026 Oct 20;15(20):8147. doi: 10.3390/jcm15208147. PMID- 42941658 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - External Validation of MRI-Based Prostate Cancer Risk Calculators in a Contemporary Pre-Biopsy mpMRI Cohort. LID - 10.3390/jcm15208146 [doi] LID - 8146 AB - Background: Multiparametric MRI (mpMRI)-integrated risk calculators may improve individualized prediction of clinically significant prostate cancer (csPCa) before biopsy. We externally validated and directly compared the MRI-based Radtke and Alberts risk calculators (Radtke-RC and Alberts-RC) in a contemporary MRI-selected biopsy cohort, assessing discrimination, calibration, and clinical utility. Methods: Consecutive patients undergoing pre-biopsy mpMRI followed by combined MRI-targeted and systematic biopsy were retrospectively identified. The primary endpoint was csPCa, defined as ISUP Grade Group >/= 2. Discrimination was assessed using receiver operating characteristic analysis, calibration using observed-to-expected (O:E) ratios, calibration-in-the-large (slope fixed at 1), calibration slopes, and calibration plots, and clinical utility using decision curve analysis. Radtke-RC validation was restricted to biopsy-naive patients, while head-to-head comparisons were restricted to biopsy-naive patients evaluable for both calculators. Threshold probabilities of 12%, 20%, and 30% were examined, with bootstrap confidence intervals for incremental net benefit. Results: Of 171 screened patients, seven had no evaluable biopsy outcome. The Radtke-RC validation cohort comprised 144 biopsy-naive patients, including 55 (38.2%) with csPCa. The Alberts-RC was evaluable in 114 patients; 50 patients could not be assessed because age, prostate volume, or PSA values were outside the online calculator's permitted input ranges. The common biopsy-naive cohort comprised 97 patients, including 34 (35.1%) with csPCa. In the model-specific cohorts, AUCs were 0.765 (95% CI 0.682-0.848) for the Radtke-RC and 0.767 (95% CI 0.672-0.861) for the Alberts-RC. In the common cohort, AUCs were 0.774 (95% CI 0.672-0.875) and 0.768 (95% CI 0.661-0.874), respectively, with no statistically significant paired difference (0.006, 95% CI -0.028 to 0.040; p = 0.726). Calibration differed substantially: the Radtke-RC overpredicted risk (mean predicted risk 48.2%; O:E 0.727), whereas the Alberts-RC underpredicted risk (18.0%; O:E 1.944). At thresholds of 12%, 20%, and 30%, the Radtke-RC classified zero, seven, and 25 patients below threshold and missed zero, zero, and four csPCa cases, respectively; corresponding values for the Alberts-RC were 42, 64, and 78 patients below threshold, with seven, 12, and 20 csPCa cases missed. Sensitivity analyses indicated less favourable Radtke-RC decision-analytic performance outside the common cohort, whereas restriction to PI-RADS 4-5 patients did not materially alter relative discrimination. Conclusions: No statistically significant difference in discrimination between the Radtke-RC and Alberts-RC was detected, although the study was not powered to establish equivalence. Despite similar discrimination, the calculators showed substantially different calibration and threshold-specific clinical consequences. In this MRI-selected population, interpretation of fixed risk thresholds should therefore account for calibration, patient selection, and the trade-off between biopsy avoidance and missed csPCa. FAU - Griessner, Hubert AU - Griessner H AUID- ORCID: 0000-0002-5499-2653 AD - Department of Urology, Paracelsus Medical University Salzburg, 5020 Salzburg, Austria. FAU - Luck, Sarah K AU - Luck SK AD - Department of Urology, Paracelsus Medical University Salzburg, 5020 Salzburg, Austria. FAU - Peters, Julia K AU - Peters JK AUID- ORCID: 0009-0005-2286-1631 AD - Department of Urology, Paracelsus Medical University Salzburg, 5020 Salzburg, Austria. FAU - Olesch, Kathrin AU - Olesch K AD - Department of Urology, Paracelsus Medical University Salzburg, 5020 Salzburg, Austria. FAU - Schlachter, Manuel AU - Schlachter M AD - Department of Urology, Paracelsus Medical University Salzburg, 5020 Salzburg, Austria. FAU - Oberhammer, Lukas AU - Oberhammer L AUID- ORCID: 0000-0002-9147-2032 AD - Department of Urology, Paracelsus Medical University Salzburg, 5020 Salzburg, Austria. FAU - Pallauf, Maximilian AU - Pallauf M AD - Department of Urology, Paracelsus Medical University Salzburg, 5020 Salzburg, Austria. FAU - Lusuardi, Lukas AU - Lusuardi L AUID- ORCID: 0000-0002-5284-7276 AD - Department of Urology, Paracelsus Medical University Salzburg, 5020 Salzburg, Austria. FAU - Haid, Philipp N AU - Haid PN AUID- ORCID: 0009-0003-7909-4862 AD - Department of Urology, Paracelsus Medical University Salzburg, 5020 Salzburg, Austria. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706581 OTO - NOTNLM OT - clinically significant prostate cancer OT - decision curve analysis OT - external validation OT - multiparametric MRI OT - prostate cancer OT - risk calculator COIS- The authors declare no competing interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:39 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - jcm15208146 [pii] AID - jcm-15-08146 [pii] AID - 10.3390/jcm15208146 [doi] PST - epublish SO - J Clin Med. 2026 Oct 20;15(20):8146. doi: 10.3390/jcm15208146. PMID- 42941654 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Clinical Scores and Machine Learning at Matched Variable Domains in Acute Appendicitis: Performance of Recommended Rule-Out Thresholds in a CT-Verified Cohort. LID - 10.3390/jcm15208141 [doi] LID - 8141 AB - Background/Objectives: Clinical scores are recommended for excluding acute appendicitis without imaging, but their rule-out thresholds were derived in patients selected on suspicion of appendicitis. This study tested whether these thresholds retain their reported sensitivity in patients selected by CT performance, estimated the incremental diagnostic value of successive layers of clinical information, and, as an exploratory aim, compared machine learning models with the scores. Methods: This single centre retrospective cohort comprised 761 emergency department patients undergoing abdominal CT for acute abdominal pain. The Alvarado score, the Appendicitis Inflammatory Response (AIR) score and the Adult Appendicitis Score (AAS) were reconstructed from data documented before CT. The reference standard was histopathology in operated patients and the index CT report with the clinical course otherwise. Models were fitted to five cumulative information layers by cross-validation, with nested algorithm selection and a temporal split. Results: Appendicitis was confirmed in 178 patients (23.4%). Sensitivity at the recommended thresholds was 0.680 (95% CI 0.609 to 0.751), 0.848 (0.791 to 0.899) and 0.697 (0.628 to 0.764), against published values of 0.99, 0.95 and 0.959, leaving 57, 27 and 54 confirmed cases in the low probability strata, and the shortfall was present in both temporal periods. Discrimination was preserved, with areas under the curve of 0.848, 0.868 and 0.881. Model discrimination rose from 0.874 with symptoms to 0.918 with laboratory values, with each increment conditional on the preceding layers and the pattern similar with a single algorithm held fixed. With nested selection, the model was not distinguishable from the AAS, and in the later temporal period its deferral threshold attained a sensitivity of 0.925. Conclusions: In a population defined by imaging rather than by clinical suspicion, the recommended rule-out thresholds did not translate. Thresholds should be re-derived and verified in the population in which imaging is to be deferred. The model analysis was exploratory and was not externally validated. FAU - Celik, Burak AU - Celik B AD - Department of General Surgery, School of Medicine, Koc University, Istanbul 34450, Turkiye. FAU - Pehlivan, Ipek AU - Pehlivan I AUID- ORCID: 0009-0004-0425-4689 AD - Department of Industrial Engineering, Faculty of Engineering, Koc University, Istanbul 34450, Turkiye. FAU - Hatipoglu, Alper AU - Hatipoglu A AUID- ORCID: 0009-0008-7619-6974 AD - School of Medicine, Koc University, Istanbul 34450, Turkiye. FAU - Dimitoka, Zeynep Ece AU - Dimitoka ZE AUID- ORCID: 0009-0008-8228-7696 AD - School of Medicine, Koc University, Istanbul 34450, Turkiye. FAU - Toprak, Safa AU - Toprak S AUID- ORCID: 0000-0002-0544-3148 AD - Department of General Surgery, School of Medicine, Koc University, Istanbul 34450, Turkiye. FAU - Ozata, Ibrahim Halil AU - Ozata IH AUID- ORCID: 0000-0001-6749-8518 AD - Department of General Surgery, School of Medicine, Koc University, Istanbul 34450, Turkiye. FAU - Balik, Emre AU - Balik E AUID- ORCID: 0000-0001-5751-1133 AD - Department of General Surgery, School of Medicine, Koc University, Istanbul 34450, Turkiye. FAU - Turkay, Metin AU - Turkay M AUID- ORCID: 0000-0003-4769-6714 AD - Department of Industrial Engineering, Faculty of Engineering, Koc University, Istanbul 34450, Turkiye. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705786 OTO - NOTNLM OT - Adult Appendicitis Score OT - Alvarado score OT - Appendicitis Inflammatory Response score OT - acute appendicitis OT - clinical prediction model OT - computed tomography OT - diagnostic accuracy OT - emergency department OT - machine learning OT - risk stratification COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:39 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - jcm15208141 [pii] AID - jcm-15-08141 [pii] AID - 10.3390/jcm15208141 [doi] PST - epublish SO - J Clin Med. 2026 Oct 20;15(20):8141. doi: 10.3390/jcm15208141. PMID- 42941653 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Perioperative Anxiety in Total Knee Arthroplasty: Clinical Consequences, Potential Mechanisms, and Management-A Narrative Review. LID - 10.3390/jcm15208140 [doi] LID - 8140 AB - Total knee arthroplasty (TKA) treats end-stage knee osteoarthritis, yet some patients report persistent pain, incomplete recovery, or dissatisfaction. Perioperative anxiety is a potentially modifiable factor associated with poorer outcomes. This narrative review examines its clinical consequences, mechanisms, and management in TKA. PubMed, Embase, Web of Science, and the China National Knowledge Infrastructure were searched without date or language restrictions through 25 September 2026. Evidence was stratified by anxiety-related selection at enrollment: studies of anxiety-selected patients provided direct evidence, whereas studies without such selection provided indirect evidence, regardless of whether anxiety was a primary, secondary, or exploratory outcome. Sixty studies underwent formal risk-of-bias appraisal. Higher anxiety was associated with greater postoperative pain and analgesic requirements and poorer patient-reported recovery and satisfaction, whereas findings for persistent pain and long-term function were less consistent. Proposed pathways include catastrophizing, kinesiophobia, reduced self-efficacy, pain sensitization, and stress-related biological processes. Pharmacological, psychoeducational, cognitive behavioral, digital, and adjunctive interventions may reduce anxiety, but direct evidence remains limited, and improvements do not consistently translate into less pain or opioid use, better function, or higher satisfaction. Anxiety may be considered in preoperative risk assessment, while standardized assessment and higher-quality trials remain needed. FAU - Zhang, Honghua AU - Zhang H AD - Department of Joint Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an 710054, China. AD - Yan'an Medical College, Yan'an University, Yan'an 716000, China. FAU - Ji, Xiang AU - Ji X AUID- ORCID: 0009-0006-2534-619X AD - Department of Joint Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an 710054, China. FAU - Sun, Xiangxiang AU - Sun X AD - Department of Joint Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an 710054, China. FAU - He, Zichao AU - He Z AD - Department of Joint Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an 710054, China. AD - Yan'an Medical College, Yan'an University, Yan'an 716000, China. FAU - Yi, Haotian AU - Yi H AD - Department of Joint Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an 710054, China. AD - Yan'an Medical College, Yan'an University, Yan'an 716000, China. FAU - Yang, Yanling AU - Yang Y AD - Yan'an Medical College, Yan'an University, Yan'an 716000, China. FAU - Bao, Huanli AU - Bao H AD - Department of Joint Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an 710054, China. FAU - Huang, Yuanchi AU - Huang Y AD - Department of Joint Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an 710054, China. FAU - Ma, Jianbing AU - Ma J AD - Department of Joint Surgery, Honghui Hospital, Xi'an Jiaotong University, Xi'an 710054, China. LA - eng GR - 2023-CX-TD-73/the Program for Innovation Team of Shaanxi Province/ PT - Journal Article PT - Review DEP - 20261020 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706237 OTO - NOTNLM OT - anxiety OT - function OT - pain OT - perioperative period OT - total knee arthroplasty COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:39 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - jcm15208140 [pii] AID - jcm-15-08140 [pii] AID - 10.3390/jcm15208140 [doi] PST - epublish SO - J Clin Med. 2026 Oct 20;15(20):8140. doi: 10.3390/jcm15208140. PMID- 42941649 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Post-Recurrence Treatment Patterns and Outcomes After Curative-Intent Multimodality Strategy in Patients with Pleural Mesothelioma: A Retrospective Cohort Study. LID - 10.3390/jcm15208137 [doi] LID - 8137 AB - Background: Disease recurrence is common after surgery for pleural mesothelioma (PM), and the evidence guiding post-recurrence management remains limited. Methods: We conducted a retrospective, single-center cohort study including patients with PM treated within a curative-intent multimodality treatment pathway including surgery aiming for a macroscopic complete resection when feasible between 2001 and 2024. Post-recurrence treatments were categorized as best supportive care only (BSC), systemic therapy alone, or local with or without systemic therapy. Survival and disease-free outcomes were analyzed in relation to treatment patterns and clinical prognostic factors. Results: Among 67 patients with a median follow-up time of 107 months from surgery, 58 (86.6%) developed disease recurrence. At first recurrence, 21 patients (36%) received local with or without systemic therapy, 26 (45%) systemic therapy alone, and 11 (19%) BSC. The median post-recurrence survival was 21.7 months for patients receiving local with or without systemic therapy, 10.8 months for systemic therapy alone, and 1.6 months for BSC. Lung-sparing surgery and a disease-free interval of at least 12 months were associated with improved post-recurrence survival and post-recurrence progression-free survival. Conclusions: In this real-world cohort of patients with PM and extended follow-up, active post-recurrence treatment was associated with longer post-recurrence survival in selected patients. However, conclusions regarding their comparative effectiveness are limited by the retrospective design, incomplete recurrence-related data, and patient selection at recurrence. FAU - Illini, Oliver AU - Illini O AUID- ORCID: 0000-0002-7322-7092 AD - Department of Respiratory and Critical Care Medicine, Klinik Floridsdorf, Vienna Healthcare Group, 1210 Vienna, Austria. AD - Karl Landsteiner Institute of Lung Research and Pulmonary Oncology, 1210 Vienna, Austria. FAU - Fabikan, Hannah AU - Fabikan H AD - Karl Landsteiner Institute of Lung Research and Pulmonary Oncology, 1210 Vienna, Austria. FAU - Berghammer, Irene AU - Berghammer I AD - Department of Respiratory and Pulmonary Diseases, Klinik Hietzing, Vienna Healthcare Group, 1130 Vienna, Austria. FAU - Krainer-Jacobs, Julie AU - Krainer-Jacobs J AUID- ORCID: 0000-0002-2953-7982 AD - Karl Landsteiner Institute of Lung Research and Pulmonary Oncology, 1210 Vienna, Austria. FAU - Schneeweiss-Worel, Amelie AU - Schneeweiss-Worel A AD - Department of Thoracic Surgery, Klinik Floridsdorf, Vienna Healthcare Group, 1210 Vienna, Austria. FAU - Ay, Leyla AU - Ay L AD - Department of Respiratory and Critical Care Medicine, Klinik Floridsdorf, Vienna Healthcare Group, 1210 Vienna, Austria. AD - Karl Landsteiner Institute of Lung Research and Pulmonary Oncology, 1210 Vienna, Austria. FAU - Moser, Daniel AU - Moser D AD - Department of Respiratory and Critical Care Medicine, Klinik Floridsdorf, Vienna Healthcare Group, 1210 Vienna, Austria. AD - Karl Landsteiner Institute of Lung Research and Pulmonary Oncology, 1210 Vienna, Austria. FAU - Weinlinger, Christoph AU - Weinlinger C AD - Karl Landsteiner Institute of Lung Research and Pulmonary Oncology, 1210 Vienna, Austria. FAU - Lang-Stoberl, Anna AU - Lang-Stoberl A AD - Karl Landsteiner Institute of Lung Research and Pulmonary Oncology, 1210 Vienna, Austria. FAU - Hochmair, Maximilian J AU - Hochmair MJ AUID- ORCID: 0000-0002-8673-2450 AD - Department of Respiratory and Critical Care Medicine, Klinik Floridsdorf, Vienna Healthcare Group, 1210 Vienna, Austria. AD - Karl Landsteiner Institute of Lung Research and Pulmonary Oncology, 1210 Vienna, Austria. FAU - Benej, Michal AU - Benej M AD - Department of Thoracic Surgery, Klinik Floridsdorf, Vienna Healthcare Group, 1210 Vienna, Austria. AD - Karl Landsteiner Institute for Thoracic Surgical Research, Klinik Floridsdorf, 1210 Vienna, Austria. FAU - Krajc, Tibor AU - Krajc T AD - Department of Thoracic Surgery, Klinik Floridsdorf, Vienna Healthcare Group, 1210 Vienna, Austria. AD - Karl Landsteiner Institute for Thoracic Surgical Research, Klinik Floridsdorf, 1210 Vienna, Austria. FAU - Klikovits, Thomas AU - Klikovits T AD - Department of Thoracic Surgery, Klinik Floridsdorf, Vienna Healthcare Group, 1210 Vienna, Austria. AD - Karl Landsteiner Institute for Thoracic Surgical Research, Klinik Floridsdorf, 1210 Vienna, Austria. FAU - Valipour, Arschang AU - Valipour A AD - Department of Respiratory and Critical Care Medicine, Klinik Floridsdorf, Vienna Healthcare Group, 1210 Vienna, Austria. AD - Karl Landsteiner Institute of Lung Research and Pulmonary Oncology, 1210 Vienna, Austria. FAU - Watzka, Stefan AU - Watzka S AUID- ORCID: 0000-0002-4149-4890 AD - Department of Thoracic Surgery, Klinik Floridsdorf, Vienna Healthcare Group, 1210 Vienna, Austria. AD - Karl Landsteiner Institute for Thoracic Surgical Research, Klinik Floridsdorf, 1210 Vienna, Austria. AD - Paracelsus Medical University, 5020 Salzburg, Austria. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706363 OTO - NOTNLM OT - disease recurrence OT - lung-sparing surgery OT - multimodality therapy OT - pleural mesothelioma OT - recurrence treatment COIS- Oliver Illini has received speaker fees and/or honoraria for advisory board participation from Boehringer Ingelheim, Eli Lilly, Johnson & Johnson, Menarini, Merck Sharp & Dohme, Pfizer, and Roche. Research grants to the institute were received from Amgen, AstraZeneca, and Takeda outside the submitted work. Daniel Moser has received speaker fees and honoraria for advisory board from GSK. Thomas Klikovits received speaker fees and/or honoraria for advisory boards from AstraZeneca, BMS, MSD, Roche, Johnson & Johnson, Amgen, Pfizer, and Medtronic. Maximilian J. Hochmair reports payment for lectures and advisory boards from Roche, BMS, MSD, Lilly, Amgen, Takeda, Pfizer, AstraZeneca, and Johnson & Johnson. Arschang Valipour has received speaker fees from AstraZeneca, Boehringer Ingelheim, Glaxo Smith Kline, Johnson & Johnson, Menarini, Merck Sharp & Dohme, Pfizer, and Roche. Stefan Watzka has received speaker fees from AstraZeneca, Bristol-Myers Squibb, and Merck Sharp & Dohme. All other authors report no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:39 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - jcm15208137 [pii] AID - jcm-15-08137 [pii] AID - 10.3390/jcm15208137 [doi] PST - epublish SO - J Clin Med. 2026 Oct 20;15(20):8137. doi: 10.3390/jcm15208137. PMID- 42941646 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Protective Effects of Serotonin, L-Carnitine, and Coenzyme Q10 on Human Sperm Cryopreservation: Functional and In Silico Evidence. LID - 10.3390/jcm15208134 [doi] LID - 8134 AB - Background: Sperm cryopreservation can impair sperm function through oxidative stress, membrane damage, mitochondrial dysfunction, and DNA fragmentation. This study evaluated the effects of supplementing a basal freezing medium with serotonin (5-HT), L-carnitine (L-C), and coenzyme Q10 (CoQ10) on post-thaw human sperm quality and explored potential molecular interactions using molecular docking. Methods: Semen samples from 63 normozoospermic volunteers were processed using the swim-up technique, and each sample was divided into five aliquots assigned to the following conditions: commercial freezing medium; basal freezing medium; basal medium supplemented with 5-HT alone; basal medium supplemented with L-C and CoQ10; and basal medium supplemented with all three compounds. Post-thaw motility, intracellular ROS, viability, and apoptosis-associated changes were assessed in all 63 donors; DNA fragmentation was assessed in a subset of 32 donors. Molecular docking explored predicted interactions of 5-HT, L-C, and coenzyme Q1 (CoQ1), a short-chain CoQ10 analogue, with selected sperm-related targets. Results: The combined formulation showed the highest median total (54%) and progressive (39%) motility, the lowest median percentage of DCF-positive spermatozoa (11.30%), and the lowest median DNA fragmentation index (2.58%). Pairwise analyses showed significant differences between the combined formulation and several comparator groups, depending on the outcome. Viability was significantly higher with the combined formulation than with basal, 5-HT-supplemented, and L-C/CoQ10-supplemented media (all Bonferroni-adjusted p < 0.001). Docking predicted interactions of 5-HT with HTR4 and SLC6A4 and of CoQ1 with CPT1A. Conclusions: Combined 5-HT, L-C, and CoQ10 supplementation was associated with favorable changes in several post-thaw sperm quality parameters. These findings support further investigation of this formulation, while the predicted molecular interactions require experimental validation. FAU - Yilmazer, Yasemin AU - Yilmazer Y AD - Department of Molecular Biology and Genetics, Istanbul Sabahattin Zaim University, Istanbul 34303, Turkiye. FAU - Cavdar, Aleyna AU - Cavdar A AD - Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, Istanbul 34210, Turkiye. AD - Department of Biochemistry, Faculty of Pharmacy, Istanbul Kent University, Istanbul 34406, Turkiye. AD - ResearchKent, Istanbul Kent University, Istanbul 34406, Turkiye. FAU - Sinirlioglu, Zeynep Aydin AU - Sinirlioglu ZA AUID- ORCID: 0000-0002-6970-6410 AD - Department of Molecular Biology and Genetics, Istanbul Sabahattin Zaim University, Istanbul 34303, Turkiye. FAU - Dogan, Sinem AU - Dogan S AUID- ORCID: 0000-0002-5577-0681 AD - Department of Obstetrics and Gynecology, Acibadem Mehmet Ali Aydinlar University, Istanbul 34303, Turkiye. FAU - Aydin, Turgut AU - Aydin T AD - Department of Obstetrics and Gynecology, Acibadem Mehmet Ali Aydinlar University, Istanbul 34303, Turkiye. LA - eng GR - Grant Number: 7220643/The Scientific and Technological Research Council of Turkiye/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705688 OTO - NOTNLM OT - cryopreservation media OT - in silico analysis OT - serotonin OT - sperm DNA fragmentation OT - sperm cryopreservation COIS- All authors declare no competing interests. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:39 PHST- 2026/08/19 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - jcm15208134 [pii] AID - jcm-15-08134 [pii] AID - 10.3390/jcm15208134 [doi] PST - epublish SO - J Clin Med. 2026 Oct 20;15(20):8134. doi: 10.3390/jcm15208134. PMID- 42941645 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Long-Term Preservation of High-Frequency Hearing After Cochlear Implantation in a Child with Substantial Residual Hearing: A Case Report. LID - 10.3390/jcm15208133 [doi] LID - 8133 AB - Background/Objectives: Cochlear implantation (CI) in children with substantial residual hearing remains challenging because of the risk of hearing loss. Partial deafness treatment and hearing preservation have traditionally focused on functionally useful low-frequency hearing, while residual hearing in the high-frequency range has not been specifically addressed in established classifications. Long-term data on preservation of substantial high-frequency residual hearing after CI in children remain limited. We present a pediatric case demonstrating 5-year preservation of high-frequency residual hearing after CI. Methods: A 14-year-old girl with bilateral sensorineural hearing loss underwent left CI with a MED-EL FLEX 28 electrode inserted through the round window. Pure-tone thresholds were assessed preoperatively and at 1, 12, 24, and 60 months postoperatively. Hearing preservation was evaluated using the Skarzynski classification, and speech perception was assessed before implantation and at 24 months. Results: Preoperatively, air-conduction thresholds were /= 3 radiation pneumonitis occurred. Conclusions: IOC-CCRT was associated with improved unadjusted survival outcomes and increased selected severe toxicities compared with IC-CCRT. Prospective randomized trials are warranted. FAU - Liu, Jiayue AU - Liu J AD - Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiation Oncology, Peking University Cancer Hospital and Institute, Beijing 100142, China. FAU - Deng, Wei AU - Deng W AUID- ORCID: 0000-0003-1778-2013 AD - Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiation Oncology, Peking University Cancer Hospital and Institute, Beijing 100142, China. FAU - Dong, Xin AU - Dong X AUID- ORCID: 0009-0008-9322-8560 AD - Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiation Oncology, Peking University Cancer Hospital and Institute, Beijing 100142, China. FAU - Zhao, Yuting AU - Zhao Y AD - Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiation Oncology, Peking University Cancer Hospital and Institute, Beijing 100142, China. FAU - Jiang, Leilei AU - Jiang L AD - Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiation Oncology, Peking University Cancer Hospital and Institute, Beijing 100142, China. FAU - Long, Liuhua AU - Long L AUID- ORCID: 0000-0001-7576-0926 AD - Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiation Oncology, Peking University Cancer Hospital and Institute, Beijing 100142, China. FAU - Teng, Yue AU - Teng Y AD - Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiation Oncology, Peking University Cancer Hospital and Institute, Beijing 100142, China. FAU - Yang, Dan AU - Yang D AD - Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiation Oncology, Peking University Cancer Hospital and Institute, Beijing 100142, China. FAU - Yu, Jiayi AU - Yu J AD - Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiation Oncology, Peking University Cancer Hospital and Institute, Beijing 100142, China. FAU - Chang, Xiao AU - Chang X AUID- ORCID: 0000-0003-0560-2441 AD - Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiation Oncology, Peking University Cancer Hospital and Institute, Beijing 100142, China. FAU - Yu, Huiming AU - Yu H AD - Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiation Oncology, Peking University Cancer Hospital and Institute, Beijing 100142, China. FAU - Shi, Anhui AU - Shi A AD - Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiation Oncology, Peking University Cancer Hospital and Institute, Beijing 100142, China. FAU - Wang, Weihu AU - Wang W AD - Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiation Oncology, Peking University Cancer Hospital and Institute, Beijing 100142, China. FAU - Yu, Rong AU - Yu R AD - Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Radiation Oncology, Peking University Cancer Hospital and Institute, Beijing 100142, China. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706056 OTO - NOTNLM OT - concurrent chemoradiotherapy OT - esophageal squamous cell carcinoma OT - immunotherapy OT - induction chemoimmunotherapy OT - induction chemotherapy OT - propensity score matching COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:39 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - jcm15208121 [pii] AID - jcm-15-08121 [pii] AID - 10.3390/jcm15208121 [doi] PST - epublish SO - J Clin Med. 2026 Oct 19;15(20):8121. doi: 10.3390/jcm15208121. PMID- 42941615 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 19 TI - Development and Internal Validation of a Preintubation Prediction Model for Postintubation Hypotension in Trauma Patients Undergoing Emergency Endotracheal Intubation: A Retrospective Cohort Study. LID - 10.3390/jcm15208102 [doi] LID - 8102 AB - Background/Objectives: Postintubation hypotension (PIH) is an important complication of emergency endotracheal intubation in trauma patients. However, the applicability of preintubation predictors of PIH in trauma patients undergoing emergency endotracheal intubation in the emergency department (ED) remains uncertain. Here, we aimed to develop and internally validate a preintubation prediction model for PIH in such patients. Methods: This retrospective cohort included trauma patients aged >/= 15 years undergoing emergency endotracheal intubation in the ED during 2015-2023. PIH was defined as systolic blood pressure < 90 mmHg within 30 min after intubation. Eight preintubation candidate predictors were evaluated using logistic regression with backward elimination. Internal validation used 2000 bootstrap resamples repeating the entire variable-selection procedure. Results: Among 348 patients, 60 (17.2%) developed PIH; 345 patients with 57 PIH events had complete data for model development. The final model retained systolic blood pressure, body temperature, hematocrit, and torso injury, with adjusted odds ratios of 1.19 per 10-mmHg lower systolic blood pressure, 1.48 per 1 degrees C lower temperature, 1.33 per 5-percentage-point lower hematocrit, and 3.26 for torso injury. The apparent and optimism-corrected areas under the receiver operating characteristic curve were 0.742 and 0.700, respectively. Conclusions: A four-variable preintubation prediction model demonstrated modest optimism-corrected discrimination and substantial model-selection uncertainty. External validation and assessment of clinical utility are required before clinical implementation. FAU - Kitsiripant, Chanatthee AU - Kitsiripant C AUID- ORCID: 0000-0003-1504-9336 AD - Department of Anesthesiology, Faculty of Medicine, Prince of Songkla University, Hat Yai 90110, Thailand. FAU - Thongkhao, Komet AU - Thongkhao K AD - Division of Trauma and Critical Care, Department of Surgery, Faculty of Medicine, Prince of Songkla University, Hat Yai 90110, Thailand. FAU - Akaraborworn, Osaree AU - Akaraborworn O AD - Division of Trauma and Critical Care, Department of Surgery, Faculty of Medicine, Prince of Songkla University, Hat Yai 90110, Thailand. FAU - Tantarattanapong, Siriwimon AU - Tantarattanapong S AD - Department of Emergency Medicine, Faculty of Medicine, Prince of Songkla University, Hat Yai 90110, Thailand. LA - eng GR - REC.67-007-8-1/Faculty of Medicine, Prince of Songkla University/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706554 OTO - NOTNLM OT - airway management OT - emergency department OT - emergency endotracheal intubation OT - postintubation hypotension OT - prediction model OT - trauma COIS- The authors declare no conflicts of interest. The funders had no role in the study design; collection, analyses, or interpretation of data; writing of the manuscript; or decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:39 PHST- 2026/10/03 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - jcm15208102 [pii] AID - jcm-15-08102 [pii] AID - 10.3390/jcm15208102 [doi] PST - epublish SO - J Clin Med. 2026 Oct 19;15(20):8102. doi: 10.3390/jcm15208102. PMID- 42941611 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 19 TI - From HPV Detection to Cancer Prevention: The Role of Infection Persistence, Genotype, and Anatomical Site in HPV-Associated Cancers. LID - 10.3390/jcm15208098 [doi] LID - 8098 AB - Background/Objectives: Most HPV infections are transient, but persistent high-risk infection, particularly with HPV16, is among the most important determinants of progression to HPV-associated precancer and cancer. The role of genotype, infection persistence, and anatomical site varies across cervical, oropharyngeal, anal, vaginal, vulvar, and penile cancers. This review synthesizes evidence to clarify how these factors shape cancer risk and prevention. Methods: This structured narrative review used iterative, topic-specific searches in PubMed, Scopus, Web of Science, and Google Scholar without a publication-year restriction and was limited to English-language sources. Search terms were adapted to each database and thematic question. Original empirical studies and secondary data analyses informed direct quantitative discussion, whereas reviews, meta-analyses, guidelines, consensus statements, and authoritative reports were used principally for context. Evidence was synthesized qualitatively because study designs, populations, anatomical sites, assays, outcomes, and clinical purposes differed substantially; no new meta-analysis was performed. Results: The most developed longitudinal and screening evidence concerned cervical cancer, whereas evidence for several non-cervical sites was smaller and more heterogeneous. HPV16 was the most important genotype in most cancers examined. The role of HPV18 and other high-risk genotypes varied depending on the cancer site, tissue type, and geographic region. Most transient infections do not lead to cancer; however, persistent high-risk HPV infection, particularly HPV16, was associated with high-grade lesions and an increased risk of cancer. Vaccination was associated with a reduction in high-grade lesions and invasive cancer in population-based studies. Self-sampling and urine testing had practical value when individuals with positive results proceeded to triage, treatment, and follow-up. Conclusions: Three key questions exist for assessing the risk of HPV-associated cancers: Where has the persistent infection occurred? Which genotype is involved? And can the healthcare system intervene before progression to cancer? In cervical cancer, vaccination, screening, triage, and treatment are linked within the most developed prevention pathway. For anal cancer, prevention is more strongly focused on high-risk groups and diagnostic capacity. In oropharyngeal cancer, HPV is important for understanding etiology and prognosis, but there is still no established population screening pathway. Evidence for vaginal, vulvar, and penile cancers must be interpreted separately by site. FAU - Jouya, Sara AU - Jouya S AUID- ORCID: 0009-0001-6769-8146 AD - Health Deputy, Birjand University of Medical Sciences, Birjand 9717853577, Iran. FAU - Shahabinia, Zahra AU - Shahabinia Z AD - Geriatric Health Research Center, Birjand University of Medical Sciences, Birjand 9717853577, Iran. FAU - Abusalah, Mai Abdel Haleem A AU - Abusalah MAHA AUID- ORCID: 0000-0002-8473-9392 AD - Department of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Al-Ahliyya Amman University, Amman 19328, Jordan. FAU - Mazidimoradi, Afrooz AU - Mazidimoradi A AD - Shiraz Health Assistant Department, Shiraz University of Medical Sciences, Shiraz 7193711351, Iran. FAU - Allahqoli, Leila AU - Allahqoli L AUID- ORCID: 0000-0002-9851-6771 AD - Faculty of Health Sciences, Cyprus International University, Nicosia 99258, Cyprus. FAU - Salehiniya, Hamid AU - Salehiniya H AD - Department of Epidemiology and Biostatistics, School of Health, Social Determinants of Health Research Center, Birjand University of Medical Sciences, Birjand 9717853577, Iran. FAU - Lee, Do-Youn AU - Lee DY AUID- ORCID: 0000-0003-0886-1713 AD - College of General Education, Kookmin University, Seoul 02707, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705806 OTO - NOTNLM OT - anatomical site OT - cancer prevention OT - genotype OT - human papillomavirus OT - infection persistence COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:39 PHST- 2026/08/30 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - jcm15208098 [pii] AID - jcm-15-08098 [pii] AID - 10.3390/jcm15208098 [doi] PST - epublish SO - J Clin Med. 2026 Oct 19;15(20):8098. doi: 10.3390/jcm15208098. PMID- 42941609 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 19 TI - Epidural Adhesiolysis and Dorsal Root Ganglion Pulsed Radiofrequency Through a Single Multifunctional Catheter: A Narrative Review and Illustrated Technical Report. LID - 10.3390/jcm15208094 [doi] LID - 8094 AB - Percutaneous epidural adhesiolysis and pulsed radiofrequency of the dorsal root ganglion (DRG-PRF) target two distinct components of chronic lumbosacral radicular pain: the restricted access of injected drugs to a nerve root shielded by epidural fibrosis, and the sensitization of the ganglion itself. A multifunctional steerable catheter carrying a bipolar electrode at its tip makes it technically feasible to perform both at the same anatomical target within a single procedure. This narrative review and illustrated technical report has three aims: to summarize the mechanistic rationale for each component (mechanical adhesiolysis, enzymatic lysis with recombinant human hyaluronidase, DRG-PRF, and the co-administered local anesthetic and corticosteroid); to describe the combined procedure step by step-landmarks, needle angles, radiofrequency parameters, injectate composition, safety checkpoints and the epidurographic endpoint-with original schematics; and to set out critically what the evidence does and does not establish. A structured but non-systematic search of MEDLINE and Embase identified a substantial, methodologically contested literature on adhesiolysis, a smaller one on hyaluronidase, and a single randomized trial of 41 patients directly comparing adhesiolysis with and without DRG-PRF. Mechanistic plausibility and technical feasibility are therefore well supported, whereas evidence of clinical benefit for the combined protocol is limited. The suggested place of the technique in the treatment pathway-as an interventional option in selected patients before surgical escalation, or when revision surgery is being considered-is a clinical proposal based on anatomical reasoning and single-center experience, not on comparative data. Comparative studies are needed to define its role. FAU - Lo Bianco, Giuliano AU - Lo Bianco G AUID- ORCID: 0000-0003-2705-1548 AD - Unit of Anesthesia, Analgesia and Percutaneous Surgery, Fondazione Istituto G. Giglio, Cefalu, 90015 Palermo, Italy. FAU - Day, Miles AU - Day M AD - Department of Anesthesiology and Pain Medicine, Texas Tech University Health Sciences Center, Lubbock, TX 79430, USA. FAU - Beall, Douglas P AU - Beall DP AUID- ORCID: 0000-0001-8340-696X AD - Comprehensive Specialty Care, Oklahoma City, OK 73112, USA. FAU - Diwan, Sudhir AU - Diwan S AUID- ORCID: 0000-0002-1245-9033 AD - Advanced Spine on Park Avenue, New York, NY 10022, USA. FAU - Abd-Elsayed, Alaa AU - Abd-Elsayed A AUID- ORCID: 0000-0002-9890-7860 AD - Department of Anesthesiology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53726, USA. FAU - Leoni, Matteo Luigi Giuseppe AU - Leoni MLG AUID- ORCID: 0000-0001-5228-3733 AD - Department of Medical and Surgical Sciences and Translational Medicine, Sapienza University of Rome, 00185 Rome, Italy. FAU - Occhigrossi, Felice AU - Occhigrossi F AUID- ORCID: 0009-0004-0992-2594 AD - Pain Therapy Unit, Azienda Ospedaliera Sandro Pertini, 00157 Rome, Italy. FAU - Mercieri, Marco AU - Mercieri M AUID- ORCID: 0000-0003-4321-6525 AD - Department of Medical and Surgical Sciences and Translational Medicine, Sapienza University of Rome, 00185 Rome, Italy. FAU - Cascella, Marco AU - Cascella M AUID- ORCID: 0000-0002-5236-3132 AD - Department of Medicine, Surgery and Dentistry, University of Salerno, 84081 Baronissi, Italy. FAU - D'Angelo, Francesco Paolo AU - D'Angelo FP AD - Unit of Anesthesia, Analgesia and Percutaneous Surgery, Fondazione Istituto G. Giglio, Cefalu, 90015 Palermo, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705801 OTO - NOTNLM OT - dorsal root ganglion OT - epidural adhesiolysis OT - epidurography OT - epidurolysis OT - hyaluronidase OT - interventional pain medicine OT - persistent spinal pain syndrome OT - pulsed radiofrequency COIS- G.L.B. has received honoraria from AlfaMed S.r.l. for lecturing and teaching activities. The remaining authors declare that they have no competing interests. All disclosures cover the 36 months preceding submission. F.P.D., who has no financial relationship with the manufacturer, had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:39 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - jcm15208094 [pii] AID - jcm-15-08094 [pii] AID - 10.3390/jcm15208094 [doi] PST - epublish SO - J Clin Med. 2026 Oct 19;15(20):8094. doi: 10.3390/jcm15208094. PMID- 42941608 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 19 TI - Association Between Admission Estimated Glomerular Filtration Rate and Long-Term Mortality in Patients with Acute Myocardial Infarction. LID - 10.3390/jcm15208096 [doi] LID - 8096 AB - Background: Reduced estimated glomerular filtration rate (eGFR) and acute kidney injury (AKI) are common in patients with ST-segment elevation myocardial infarction (STEMI) and adversely influence prognosis. This retrospective cohort study evaluated the association between admission eGFR and one-year mortality following STEMI, accounting for admission AKI and cardiovascular risk factors. Methods: Consecutive adults (n = 2903) admitted with STEMI undergoing primary percutaneous coronary intervention (PCI) at a tertiary center were analyzed, selected from 2949 initially screened patients after excluding those treated conservatively or with thrombolysis (n = 14), those with a final discharge diagnosis other than STEMI (n = 21), and early deaths within 24 h of presentation (n = 11). Admission eGFR was calculated using the 2021 CKD-EPI creatinine equation and categorized into quintiles. The primary outcome was all-cause one-year mortality, assessed using multivariable logistic regression and secondary Kaplan-Meier survival analysis. Results: Both one-year and 30-day crude mortality decreased markedly across increasing eGFR quintiles (p < 0.001), a pattern that was maintained among patients presenting without admission AKI (30-day mortality: Q5 4.4% vs. Q1 0.0%, p < 0.001). In crude analysis, 30-day mortality was 9.1% in the lowest eGFR quintile overall. Multivariable logistic regression identified advancing age (OR 1.05 per year, p < 0.001) and admission AKI (OR 7.07, p < 0.001) as the dominant independent predictors of one-year mortality, whereas individual eGFR quintiles were attenuated after adjustment (global Wald p = 0.047). Conclusions: Lower admission eGFR is significantly associated with one-year mortality after STEMI in crude and unadjusted analyses, but its predictive contribution is substantially attenuated following multivariable adjustment for advancing age and admission AKI, which serve as the dominant prognostic determinants. Rather than acting as a strong isolated independent predictor, admission renal function primarily serves as an indicator of baseline cardiorenal reserve and baseline patient vulnerability. FAU - Vishnevsky, Guy AU - Vishnevsky G AUID- ORCID: 0000-0001-9928-1591 AD - Department of Cardiology, Tel Aviv Sourasky University Medical Center, Tel Aviv 6423906, Israel. AD - Gray Faculty of Medical & Health Sciences, Tel Aviv University, Tel Aviv 6997801, Israel. AD - Internal Medicine H, Tel Aviv Sourasky University Medical Center, Tel Aviv 6423906, Israel. FAU - Wolf, Ammie AU - Wolf A AUID- ORCID: 0009-0003-8456-542X AD - Department of Cardiology, Tel Aviv Sourasky University Medical Center, Tel Aviv 6423906, Israel. AD - Gray Faculty of Medical & Health Sciences, Tel Aviv University, Tel Aviv 6997801, Israel. FAU - Perez, Yoav AU - Perez Y AD - Department of Cardiology, Tel Aviv Sourasky University Medical Center, Tel Aviv 6423906, Israel. AD - Gray Faculty of Medical & Health Sciences, Tel Aviv University, Tel Aviv 6997801, Israel. FAU - Frydman, Shir AU - Frydman S AUID- ORCID: 0000-0002-8882-9109 AD - Department of Cardiology, Tel Aviv Sourasky University Medical Center, Tel Aviv 6423906, Israel. AD - Gray Faculty of Medical & Health Sciences, Tel Aviv University, Tel Aviv 6997801, Israel. FAU - Khoury, Shafik AU - Khoury S AD - Department of Cardiology, Tel Aviv Sourasky University Medical Center, Tel Aviv 6423906, Israel. AD - Gray Faculty of Medical & Health Sciences, Tel Aviv University, Tel Aviv 6997801, Israel. FAU - Elbassel, Reem AU - Elbassel R AD - Department of Cardiology, Tel Aviv Sourasky University Medical Center, Tel Aviv 6423906, Israel. AD - Gray Faculty of Medical & Health Sciences, Tel Aviv University, Tel Aviv 6997801, Israel. FAU - Topilsky, Yan AU - Topilsky Y AD - Department of Cardiology, Tel Aviv Sourasky University Medical Center, Tel Aviv 6423906, Israel. AD - Gray Faculty of Medical & Health Sciences, Tel Aviv University, Tel Aviv 6997801, Israel. FAU - Shacham, Yacov AU - Shacham Y AD - Department of Cardiology, Tel Aviv Sourasky University Medical Center, Tel Aviv 6423906, Israel. AD - Gray Faculty of Medical & Health Sciences, Tel Aviv University, Tel Aviv 6997801, Israel. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705727 OTO - NOTNLM OT - ST-segment elevation myocardial infarction (STEMI) OT - acute kidney injury (AKI) OT - chronic kidney disease (CKD) OT - eGFR COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:39 PHST- 2026/08/16 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - jcm15208096 [pii] AID - jcm-15-08096 [pii] AID - 10.3390/jcm15208096 [doi] PST - epublish SO - J Clin Med. 2026 Oct 19;15(20):8096. doi: 10.3390/jcm15208096. PMID- 42941606 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 19 TI - Carbapenem-Resistant Acinetobacter baumannii Isolation and In-Hospital Mortality in Adults Hospitalized with COVID-19 in Romania: A Retrospective Single-Center Cohort Study. LID - 10.3390/jcm15208093 [doi] LID - 8093 AB - Background/Objectives: We assessed the frequency, timing and management of carbapenem-resistant Acinetobacter baumannii (CRAB) isolation and its association with in-hospital mortality in adults hospitalized with COVID-19. Methods: This retrospective single-center cohort study included all 395 adults admitted from 12 July to 25 September 2022. The primary analysis compared in-hospital mortality by CRAB isolation in the full cohort, with Firth-penalized adjustment for age and admission SpO(2). Treatment and case-level data, complete for 95 chart-verified patients, were analyzed secondarily; isolates were classified by onset (NHSN criteria) and respiratory isolates adjudicated as infection or colonization. Results: CRAB was isolated in 33/395 patients (8.4%). Mortality was 36.4% (12/33) with CRAB isolation versus 11.6% (42/362) without (crude OR 4.35, 95% CI 1.81-10.02; adjusted OR 3.82, 95% CI 1.62-9.02). In the detailed-data subset (8/95 with CRAB), isolates were first detected on hospital days 2-6 and four were community-onset; four of seven respiratory isolates represented colonization. All eight received empirical carbapenem plus colistin after specimen collection, including full 9-10-day colistin-based courses in the four colonized patients. Conclusions: CRAB isolation was associated with higher in-hospital mortality after adjustment for baseline severity, but selective sampling precludes causal inference. Early detection suggests frequent carriage on admission; treatment of colonization was the main stewardship target, supporting admission screening and diagnostic stewardship. FAU - Bujor, Cristiana Georgeta AU - Bujor CG AUID- ORCID: 0009-0005-2467-9091 AD - Department IV-Biochemistry and Pharmacology, Faculty of Medicine, "Victor Babes" University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square 2, 300041 Timisoara, Romania. FAU - Dinuti, Marilena AU - Dinuti M AD - Department IV-Biochemistry and Pharmacology, Faculty of Medicine, "Victor Babes" University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square 2, 300041 Timisoara, Romania. FAU - Sfrijan, Felicia AU - Sfrijan F AUID- ORCID: 0009-0008-4048-1183 AD - Department IV-Biochemistry and Pharmacology, Faculty of Medicine, "Victor Babes" University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square 2, 300041 Timisoara, Romania. LA - eng GR - Victor Babes University of Medicine and Pharmacy Timisoara/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705631 OTO - NOTNLM OT - Acinetobacter baumannii OT - COVID-19 OT - antimicrobial stewardship OT - carbapenem resistance OT - colistin OT - colonization OT - in-hospital mortality OT - retrospective cohort study COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:39 PHST- 2026/09/13 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - jcm15208093 [pii] AID - jcm-15-08093 [pii] AID - 10.3390/jcm15208093 [doi] PST - epublish SO - J Clin Med. 2026 Oct 19;15(20):8093. doi: 10.3390/jcm15208093. PMID- 42941603 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 18 TI - Clinical Risk Factors and Inflammatory Markers as Predictors of Postoperative Delirium in Patients Undergoing Acute Type A Aortic Dissection Surgery. LID - 10.3390/jcm15208090 [doi] LID - 8090 AB - Background: Postoperative delirium (POD) is a common and serious neurological complication following acute type A aortic dissection (ATAAD) repair, contributing to increased morbidity and mortality. Given the critical role of systemic inflammation in POD pathogenesis, this study aimed to evaluate perioperative inflammatory profiles in patients undergoing ATAAD repair and to develop a predictive nomogram for early POD risk stratification. Methods: This retrospective, single-center study included 355 patients who underwent total arch replacement for ATAAD. Serial inflammatory markers were assessed across multiple perioperative time points. A two-stage variable selection process, incorporating variance inflation factor (VIF) and the Boruta algorithm, was utilized to identify robust predictors. A predictive nomogram was constructed via multivariable logistic regression. Model performance was assessed using the area under the receiver operating characteristic curve (AUC), calibration plots, and decision curve analysis (DCA). Internal validation was performed using 1000 bootstrap resamples. Results: POD occurred in 29.86% of the cohort. Patients who developed POD had higher inflammatory marker levels at several individual postoperative time points compared to the non-delirium group. The final predictive model integrated 11 key variables: age, EuroSCORE I, CPB time, SIRI_6h, PLR_6h, IL-10_6h, IL-8_6h, IL-8_pre, IL-6_6h, IL-6_pre, and PCT_6h. The nomogram demonstrated acceptable internal discrimination with an AUC of 0.835 (95% CI: 0.794-0.876) and an optimism-corrected AUC of 0.811. Notably, IL-6_6h and PCT_6h emerged as strong independent risk factors, while SIRI_6h demonstrated an inverse association with POD. Calibration and DCA analyses confirmed the model's acceptable performance and potential clinical utility, though independent external validation remains essential prior to any clinical translation. An exploratory, web-based risk calculator was developed to facilitate bedside implementation. Conclusions: This study develops and internally validates an exploratory biomarker-based prediction model for POD in ATAAD patients. By integrating early inflammatory profiles with established clinical variables, this preliminary model provides a preliminary foundation for early risk stratification, though rigorous prospective multicenter external validation is required before real-world clinical implementation can be considered. FAU - Cao, Xiantong AU - Cao X AUID- ORCID: 0000-0003-2912-5942 AD - Department of Cardiovascular Surgery, First Affiliated Hospital of Xi'an Jiaotong University, No. 277 Yanta West Road, Xi'an 710061, China. FAU - Wang, Botao AU - Wang B AD - Department of Cardiovascular Surgery, First Affiliated Hospital of Xi'an Jiaotong University, No. 277 Yanta West Road, Xi'an 710061, China. FAU - Yang, Huan AU - Yang H AD - Department of Cardiovascular Surgery, First Affiliated Hospital of Xi'an Jiaotong University, No. 277 Yanta West Road, Xi'an 710061, China. FAU - Wu, Yujing AU - Wu Y AD - Department of Emergency Medicine, First Affiliated Hospital of Xi'an Jiaotong University, No. 277 Yanta West Road, Xi'an 710061, China. FAU - Zhang, Yongjian AU - Zhang Y AD - Department of Cardiovascular Surgery, First Affiliated Hospital of Xi'an Jiaotong University, No. 277 Yanta West Road, Xi'an 710061, China. FAU - Zhong, Liang AU - Zhong L AD - Department of Cardiovascular Surgery, First Affiliated Hospital of Xi'an Jiaotong University, No. 277 Yanta West Road, Xi'an 710061, China. FAU - Ma, Tao AU - Ma T AD - Department of Cardiovascular Surgery, First Affiliated Hospital of Xi'an Jiaotong University, No. 277 Yanta West Road, Xi'an 710061, China. FAU - Shi, Tao AU - Shi T AUID- ORCID: 0000-0002-1221-5100 AD - Department of Cardiovascular Surgery, First Affiliated Hospital of Xi'an Jiaotong University, No. 277 Yanta West Road, Xi'an 710061, China. FAU - Zhang, Zhanqin AU - Zhang Z AUID- ORCID: 0000-0003-1088-3372 AD - Department of Cardiovascular Surgery, First Affiliated Hospital of Xi'an Jiaotong University, No. 277 Yanta West Road, Xi'an 710061, China. LA - eng PT - Journal Article DEP - 20261018 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705684 OTO - NOTNLM OT - acute type A aortic dissection OT - interleukin-6 OT - postoperative delirium OT - predictive nomogram OT - procalcitonin OT - systemic inflammation response index COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:39 PHST- 2026/08/28 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - jcm15208090 [pii] AID - jcm-15-08090 [pii] AID - 10.3390/jcm15208090 [doi] PST - epublish SO - J Clin Med. 2026 Oct 18;15(20):8090. doi: 10.3390/jcm15208090. PMID- 42941601 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 18 TI - Repeat and Redo Microdissection Testicular Sperm Extraction (Micro-TESE) in Non-Obstructive Azoospermia: A Narrative Review. LID - 10.3390/jcm15208088 [doi] LID - 8088 AB - Microdissection testicular sperm extraction (micro-TESE) is the standard surgical approach for sperm retrieval in men with non-obstructive azoospermia (NOA). However, further procedures may be required either after successful retrieval to support additional intracytoplasmic sperm injection cycles or as salvage treatment following initial failure. This narrative review evaluates outcomes of repeat micro-TESE after previous success and redo micro-TESE after failure, focusing on sperm retrieval rates (SRRs), predictors of success, and strategies proposed to optimize outcomes before reoperation. Repeat procedures following successful retrieval generally show high SRRs, with one large series reporting rates of 73.9-92.5% at subsequent attempts; however, these estimates reflect progressively selected populations and should not be interpreted as independent per-attempt probabilities. In contrast, redo micro-TESE after initial failure achieves sperm retrieval in approximately 10-21% of cases in most series, although higher rates have occasionally been reported. Previous retrieval outcome and testicular histopathology appear most informative for counselling, whereas serum FSH, testicular volume, genetics, and the interval between procedures have inconsistent predictive value. Hormonal pretreatment may modify the intratesticular environment, but evidence for improved redo retrieval remains limited and heterogeneous. Antioxidant therapy is biologically plausible but has not been adequately evaluated in this setting. Technical factors, including the adequacy of the initial procedure, surgical expertise, and laboratory processing, may also influence outcomes. Prospective controlled studies with standardized reporting are needed to define optimal patient selection and management before repeat or redo micro-TESE. FAU - Douvli, Erriketi AU - Douvli E AUID- ORCID: 0009-0007-2175-3641 AD - Urology Department, Ioannina University, 45500 Ioannina, Greece. FAU - Zachariou, Athanasios AU - Zachariou A AUID- ORCID: 0000-0002-5287-4450 AD - Urology Department, Ioannina University, 45500 Ioannina, Greece. FAU - Giannakis, Ioannis AU - Giannakis I AD - Urology Department, Ioannina University, 45500 Ioannina, Greece. FAU - Koukos, Sotirios AU - Koukos S AD - Urology Department, Ioannina University, 45500 Ioannina, Greece. FAU - Kaltsas, Aris AU - Kaltsas A AUID- ORCID: 0000-0003-4651-312X AD - 3rd Urology Department, Athens University, 12462 Athens, Greece. FAU - Sofikiti, Harikleia Maria AU - Sofikiti HM AD - Urology Department, Ioannina University, 45500 Ioannina, Greece. FAU - Baltogiannis, Michail AU - Baltogiannis M AD - Urology Department, Ioannina University, 45500 Ioannina, Greece. FAU - Dimitriadis, Fotios AU - Dimitriadis F AUID- ORCID: 0000-0003-1198-9144 AD - 1st Urology Department, Aristotle Thessaloniki University, 54635 Thessaloniki, Greece. FAU - Morizane, Suichi AU - Morizane S AD - Division of Urology, Department of Surgery, Faculty of Medicine, Tottori University, Yonago 683-8504, Tottori, Japan. FAU - Sofikitis, Nikolaos AU - Sofikitis N AD - Urology Department, Ioannina University, 45500 Ioannina, Greece. LA - eng PT - Journal Article PT - Review DEP - 20261018 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706044 OTO - NOTNLM OT - antioxidants OT - hormonal therapy OT - microdissection testicular sperm extraction OT - non-obstructive azoospermia OT - redo micro-TESE OT - repeat micro-TESE OT - sperm retrieval rate COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:39 PHST- 2026/08/29 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - jcm15208088 [pii] AID - jcm-15-08088 [pii] AID - 10.3390/jcm15208088 [doi] PST - epublish SO - J Clin Med. 2026 Oct 18;15(20):8088. doi: 10.3390/jcm15208088. PMID- 42941595 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 18 TI - Current Practices of Prone Positioning in ARDS Reported by European ICU Clinicians (CUPIDO)-A Cross-Sectional Questionnaire-Based Survey. LID - 10.3390/jcm15208082 [doi] LID - 8082 AB - Background: Prone positioning improves survival in patients with moderate to severe acute respiratory distress syndrome (ARDS), yet its implementation in routine clinical practice remains poorly characterized. We conducted a survey to assess contemporary prone positioning practices and their heterogeneity in the management of ARDS across European intensive care units (ICUs). Methods: A cross-sectional, questionnaire-based survey was conducted among clinicians involved in ARDS management between March and August 2025. The survey was developed by a panel of experts in intensive care medicine and underwent testing for face and content validity prior to dissemination. The questionnaire was distributed through European networks, including the European Society of Intensive Care Medicine (ESICM), the PROtective VEntilation Network (PROVE Network), the European Society of Anaesthesiology and Intensive Care (ESAIC), and national societies. Results: Out of 592 eligible responses, 451 responses were complete and from European clinicians (66.1% male), with the largest contributions being from Italy (28.6%), Germany (18.4%) and Austria (11.5%). Of these, 446 respondents (98.9%) reported performing prone positioning. Initiation thresholds for prone positioning varied, most commonly a ratio of arterial partial pressure of oxygen to fraction of inspired oxygen (PaO(2)/FiO(2)) /=65 years remained. Linear regression of log-transformed TSH included age per five years and sex, with HC3 robust standard errors. Sensitivity models added comorbidities and medication count and applied the institution-specific TSH and fT4 reference intervals. Exploratory quantile regression used bootstrap confidence intervals (CIs) and formal comparisons of percentile-specific coefficients. Results: Mean age was 73.9 +/- 6.5 years; 122 participants (59.2%) were women. Median TSH was 1.80 mIU/L (interquartile range, 1.25-2.61) and did not differ across age groups (p = 0.151). The primary age estimate was -6.6% per five years (95% CI, -13.1% to +0.5%; p = 0.066) and was unchanged after comorbidity adjustment (-6.7%; p = 0.077), but attenuated when analysis was restricted to the institutional TSH interval (-3.2%; p = 0.274). Women had 25.5% higher TSH in the primary model (95% CI, +1.3% to +55.5%; p = 0.037). An exploratory 10th-percentile age estimate (-19.6%; 95% bootstrap CI, -37.4% to -6.6%) was not supported by a joint test of percentile differences (p = 0.13). Conclusions: No statistically significant age association was demonstrated in this selected cohort. The primary analysis indicated higher TSH in women, although precision varied across restricted analyses. Isolated TSH results in older outpatients should be confirmed and interpreted in a clinical context; larger prospective studies are needed. FAU - Akbas, Sibel AU - Akbas S AUID- ORCID: 0000-0001-9620-4507 AD - Division of Geriatrics, Department of Internal Medicine, Kocaeli City Hospital, 41060 Izmit, Turkiye. FAU - Uckun, Aysegul AU - Uckun A AUID- ORCID: 0009-0005-1457-785X AD - Department of Medical Oncology, Dr. Abdurrahman Yurtaslan Ankara Oncology Training and Research Hospital, University of Health Sciences, 06200 Ankara, Turkiye. FAU - Gumuscubuk, Oguzcan AU - Gumuscubuk O AUID- ORCID: 0000-0001-7462-7453 AD - Department of Geriatric Medicine, Sincan Training and Research Hospital, 06949 Ankara, Turkiye. FAU - Yalcin, Ahmet AU - Yalcin A AUID- ORCID: 0000-0001-9472-2212 AD - Division of Geriatrics, Department of Internal Medicine, Faculty of Medicine, Ankara University, 06230 Ankara, Turkiye. FAU - Varli, Murat AU - Varli M AUID- ORCID: 0000-0003-1176-5255 AD - Division of Geriatrics, Department of Internal Medicine, Faculty of Medicine, Ankara University, 06230 Ankara, Turkiye. FAU - Atmis, Volkan AU - Atmis V AUID- ORCID: 0000-0002-0080-6448 AD - Division of Geriatrics, Department of Internal Medicine, Faculty of Medicine, Ankara University, 06230 Ankara, Turkiye. LA - eng PT - Journal Article DEP - 20261017 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705884 OTO - NOTNLM OT - aging OT - geriatrics OT - older adults OT - sex differences OT - thyroid-stimulating hormone OT - thyrotropin COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:39 PHST- 2026/09/06 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - jcm15208059 [pii] AID - jcm-15-08059 [pii] AID - 10.3390/jcm15208059 [doi] PST - epublish SO - J Clin Med. 2026 Oct 17;15(20):8059. doi: 10.3390/jcm15208059. PMID- 42941568 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - Autofluorescence Imaging During Surgery for Orthopedic Infection: A Prospective Feasibility Case Series. LID - 10.3390/jcm15208055 [doi] LID - 8055 AB - Background/Objectives: Intraoperative assessment of infected and nonviable tissue remains challenging in orthopedic surgery. This prospective case series evaluated the technical feasibility of VELscope((R)) autofluorescence imaging during surgery for orthopedic infection. Methods: Thirteen patients undergoing surgery for hip or knee periprosthetic joint infection or spondylodiscitis were included. Autofluorescence was assessed qualitatively before and after conventional debridement by the operating surgeon, without quantitative thresholds or independent, blinded image assessment. Microbiological and histopathological findings from selected sampled areas were summarized at the patient level. Results: Imaging was performed in all 13 cases without reported device-related complications. Altered autofluorescence was observed at baseline in all cases and was no longer observed after debridement judged macroscopically complete in 12 of 13 cases (92.3%; 95% CI 66.7-98.6). Sampling from areas with altered autofluorescence yielded culture growth in 11 of 13 cases (84.6%; 95% CI 57.8-95.7) and reported active inflammation in 10 of 13 cases (76.9%; 95% CI 49.7-91.8). Conclusions: Intraoperative autofluorescence imaging was technically feasible in this small series. The imaging and laboratory findings are exploratory. Diagnostic accuracy, reproducibility, debridement completeness, and clinical benefit remain to be established. FAU - Babasiz, Mari AU - Babasiz M AUID- ORCID: 0009-0000-3273-8163 AD - Department of Orthopedics and Trauma Surgery, University Hospital Bonn, University of Bonn, 53113 Bonn, Germany. FAU - Koller, Jessica AU - Koller J AD - Department of Orthopedics and Trauma Surgery, University Hospital Bonn, University of Bonn, 53113 Bonn, Germany. FAU - Tuburu, Tamaradoubra T AU - Tuburu TT AD - Department of Orthopedics and Trauma Surgery, University Hospital Bonn, University of Bonn, 53113 Bonn, Germany. FAU - Strauss, Andreas Christian AU - Strauss AC AUID- ORCID: 0000-0001-7777-3253 AD - Department of Orthopedics and Trauma Surgery, University Hospital Bonn, University of Bonn, 53113 Bonn, Germany. FAU - Assaf, Elio AU - Assaf E AUID- ORCID: 0000-0002-3105-0356 AD - Department of Orthopedics and Trauma Surgery, University Hospital Bonn, University of Bonn, 53113 Bonn, Germany. FAU - Jaenisch, Max AU - Jaenisch M AUID- ORCID: 0000-0001-5998-0224 AD - Department of Orthopedics and Trauma Surgery, University Hospital Bonn, University of Bonn, 53113 Bonn, Germany. FAU - Wirtz, Dieter C AU - Wirtz DC AUID- ORCID: 0000-0002-6189-5114 AD - Department of Orthopedics and Trauma Surgery, University Hospital Bonn, University of Bonn, 53113 Bonn, Germany. FAU - Schildberg, Frank A AU - Schildberg FA AUID- ORCID: 0000-0003-0797-1945 AD - Department of Orthopedics and Trauma Surgery, University Hospital Bonn, University of Bonn, 53113 Bonn, Germany. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705935 OTO - NOTNLM OT - PJI OT - VELscope OT - autofluorescence imaging OT - osteomyelitis OT - spondylodiscitis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:39 PHST- 2026/08/26 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - jcm15208055 [pii] AID - jcm-15-08055 [pii] AID - 10.3390/jcm15208055 [doi] PST - epublish SO - J Clin Med. 2026 Oct 16;15(20):8055. doi: 10.3390/jcm15208055. PMID- 42941565 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - Comparison of Neurodynamic Test Response Between Patients with Frequent Episodic Tension-Type Headache and Migraine: An Exploratory Comparative Study. LID - 10.3390/jcm15208052 [doi] LID - 8052 AB - Background/Objectives: Although migraine and frequent episodic tension-type headache (FETTH) may share sensory features, direct comparisons of responses to neurodynamic loading are scarce. Establishing whether responses differ may help distinguish shared from phenotype-specific features and guide future diagnostic research. The aim of this exploratory secondary analysis was to compare range-of-motion and pain responses during the Long Seated Slump Test (LSST), Seated Slump Test (SST), and Straight Leg Raise (SLR) in participants with migraine and FETTH and to explore their potential discriminatory performance. Methods: This cross-sectional observational secondary analysis included participants with FETTH (n = 31) and migraine (n = 33) classified by a neurologist according to ICHD-3 criteria. LSST outcomes were sacral flexion, cervical flexion, and pain at maximal cervical flexion; SST outcomes were active knee-extension angle and pain at maximal active knee extension in dominant and non-dominant limbs; and SLR outcomes were hip-flexion angle and pain intensity at a low-pain point (O(1)) and high-pain point (O(2)), assessed bilaterally with the ankle at 30 degrees plantar flexion and neutral (0 degrees ). Results: In the unadjusted exploratory analyses, FETTH showed greater LSST sacral flexion than migraine (adjusted mean difference = 13.56 degrees , 95% CI 8.58 to 18.54), whereas migraine showed greater pain at maximal LSST cervical flexion (adjusted mean difference = -1.45 NPRS points, 95% CI -2.42 to -0.48). An unadjusted between-group difference was also observed for the dominant-limb SLR angle at the high-pain point with the ankle neutral (adjusted mean difference = -10.17 degrees , 95% CI -18.95 to -1.39). After Holm adjustment across the 23 neurodynamic ANCOVA comparisons, only LSST sacral flexion remained statistically significant. LSST sacral flexion also yielded the highest apparent AUC (0.881), with 78.8% sensitivity and 90.3% specificity at the same-sample exploratory cut-off. Conclusions: Neurodynamic responses differed selectively, with the clearest exploratory signal involving LSST sacral flexion. The same-sample cut-off is not a validated diagnostic threshold, and the sex imbalance between groups limits attribution to headache phenotype. Independent validation and assessment of incremental value beyond ICHD-3 classification are required before clinical use. FAU - Caamano-Barrios, Leandro H AU - Caamano-Barrios LH AUID- ORCID: 0009-0005-5834-3385 AD - Escuela Universitaria Gimbernat Cantabria, Adscrita la Universidad de Cantabria, 39300 Torrelavega, Spain. FAU - Ostolaza-Guitian, Ana AU - Ostolaza-Guitian A AD - Escuela Universitaria Gimbernat Cantabria, Adscrita la Universidad de Cantabria, 39300 Torrelavega, Spain. FAU - Cuevas-Rodriguez, Ruben AU - Cuevas-Rodriguez R AD - Escuela Universitaria Gimbernat Cantabria, Adscrita la Universidad de Cantabria, 39300 Torrelavega, Spain. FAU - Valera-Calero, Juan Antonio AU - Valera-Calero JA AUID- ORCID: 0000-0002-3379-8392 AD - Department of Physiotherapy, Faculty of Nursery, Physiotherapy and Podiatry, Universidad Complutense de Madrid, 28040 Madrid, Spain. FAU - Galan-Del-Rio, Fernando AU - Galan-Del-Rio F AUID- ORCID: 0000-0002-3515-2616 AD - Department of Physical Therapy, Occupational Therapy, Rehabilitation and Physical Medicine, Universidad Rey Juan Carlos, 28922 Alcorcon, Spain. AD - Grupo de Investigacion de Alto Rendimiento en Evaluacion Multidimensional y Tratamiento del Dolor Cronico, Universidad Rey Juan Carlos, 28922 Alcorcon, Spain. FAU - Sanchez-Jorge, Sandra AU - Sanchez-Jorge S AUID- ORCID: 0000-0001-6509-3322 AD - Faculty of Health Sciences, Francisco de Vitoria University, 28040 Madrid, Spain. FAU - Ortega-Santiago, Ricardo AU - Ortega-Santiago R AUID- ORCID: 0000-0003-3271-5767 AD - Department of Physical Therapy, Occupational Therapy, Rehabilitation and Physical Medicine, Universidad Rey Juan Carlos, 28922 Alcorcon, Spain. AD - Grupo de Investigacion de Alto Rendimiento en Evaluacion Multidimensional y Tratamiento del Dolor Cronico, Universidad Rey Juan Carlos, 28922 Alcorcon, Spain. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706521 OTO - NOTNLM OT - migraine OT - nerve tissues OT - neurodynamic OT - sensitization OT - tension-type headache COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:39 PHST- 2026/09/22 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - jcm15208052 [pii] AID - jcm-15-08052 [pii] AID - 10.3390/jcm15208052 [doi] PST - epublish SO - J Clin Med. 2026 Oct 16;15(20):8052. doi: 10.3390/jcm15208052. PMID- 42941556 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - Association of Serum Visfatin Levels with Coronary Artery Disease and Biochemical Parameters in Patients Undergoing Maintenance Hemodialysis. LID - 10.3390/jcm15208042 [doi] LID - 8042 AB - Background: Cardiovascular disease is a major cause of morbidity and mortality in patients with end-stage kidney disease receiving maintenance hemodialysis. Visfatin is a pro-inflammatory adipokine involved in metabolic and inflammatory pathways; however, its relationship with established coronary artery disease (CAD) in hemodialysis patients remains uncertain. This study evaluated the associations of serum visfatin concentrations with hemodialysis status, clinically documented CAD, and selected metabolic and biochemical parameters. Methods: This cross-sectional study included 130 non-diabetic adults classified into four groups: healthy controls (n = 40), participants with preserved renal function and established CAD (n = 28), maintenance hemodialysis patients without CAD (n = 30), and maintenance hemodialysis patients with established CAD (n = 32). Demographic, anthropometric, and laboratory variables were recorded. Serum visfatin concentrations were measured using an enzyme-linked immunosorbent assay. Between-group comparisons and exploratory correlation analyses were performed. Analysis of covariance was additionally used to assess between-group differences in visfatin after adjustment for age, sex, hypertension, and BMI, with body fat percentage substituted for BMI in a sensitivity analysis. Results: Median serum visfatin concentration was higher in the pooled hemodialysis groups than in the pooled non-hemodialysis groups (35.75 vs. 30.51 ng/mL, p = 0.014). In unadjusted pairwise comparisons, healthy controls had lower visfatin concentrations than participants with preserved renal function and CAD (p = 0.007), hemodialysis patients without CAD (p = 0.001), and hemodialysis patients with CAD (p = 0.004). However, visfatin concentrations did not differ between hemodialysis patients with and without established CAD (p = 0.972). After adjustment for age, sex, hypertension, and BMI, the overall between-group effect was no longer statistically significant (F(3, 122)= 2.054, p = 0.110, partial eta(2) = 0.048). The result was similar in the sensitivity analysis replacing BMI with body fat percentage (p = 0.060). In exploratory analyses of the pooled hemodialysis population, visfatin was positively correlated with fasting insulin (r = 0.269, p = 0.039), HOMA-IR (r = 0.277, p = 0.033), and serum uric acid (r = 0.263, p = 0.039). Conclusions: Higher serum visfatin concentrations were observed in patients receiving maintenance hemodialysis in unadjusted analyses, but the overall between-group difference was attenuated after adjustment for demographic and body-composition variables. Visfatin did not distinguish hemodialysis patients according to established CAD status. These findings do not demonstrate an independent association of circulating visfatin with either hemodialysis status or clinically documented CAD. Given the cross-sectional design and exploratory nature of the correlation analyses, further prospective studies with larger samples and standardized assessment of coronary disease burden are warranted. FAU - Aktas, Nimet AU - Aktas N AD - Department of Nephrology, University of Health Sciences, Bursa Yuksek Ihtisas Training and Research Hospital, 16310 Bursa, Turkiye. FAU - Gullulu, Mustafa AU - Gullulu M AD - Department of Nephrology, Faculty of Medicine, Bursa Uludag University, 16059 Bursa, Turkiye. FAU - Sensoy, Nazife Nur Ozer AU - Sensoy NNO AUID- ORCID: 0000-0001-8809-2250 AD - Department of Nephrology, University of Health Sciences, Bursa Yuksek Ihtisas Training and Research Hospital, 16310 Bursa, Turkiye. FAU - Ersoy, Alparslan AU - Ersoy A AUID- ORCID: 0000-0002-0710-0923 AD - Department of Nephrology, Faculty of Medicine, Bursa Uludag University, 16059 Bursa, Turkiye. FAU - Sensoy, Baris AU - Sensoy B AD - Department of Cardiology, University of Health Sciences, Bursa Yuksek Ihtisas Training and Research Hospital, 16059 Bursa, Turkiye. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706501 OTO - NOTNLM OT - adipokines OT - biomarkers OT - chronic kidney disease OT - coronary artery disease OT - hemodialysis OT - visfatin COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:39 PHST- 2026/08/04 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - jcm15208042 [pii] AID - jcm-15-08042 [pii] AID - 10.3390/jcm15208042 [doi] PST - epublish SO - J Clin Med. 2026 Oct 16;15(20):8042. doi: 10.3390/jcm15208042. PMID- 42941555 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - The Learning Curve in Robotic HPB Surgery: Myth, Metrics, and Misinterpretations. LID - 10.3390/jcm15208043 [doi] LID - 8043 AB - Background: The evolution of robotic surgery has widened the indications for minimally invasive hepatopancreatobiliary (HPB) surgery, making possible the execution of more complicated cases. Within this context, the learning curve concept is now an essential factor when assessing surgical training and expertise, as well as patient safety within a robotic HPB program. Nevertheless, existing methodologies often make use of procedure volume and surrogate intraoperative measures, which may be an insufficient way to define surgical competence. Methods: This narrative review examines the learning curve concept in robotic HPB surgery, focusing on existing performance metrics, methodological limitations, procedural complexity, risk adjustment, and the influence of team-related and institutional factors. Results: The time of operation, the estimated blood loss, the rate of conversion, and postoperative morbidity are frequently applied to assess learning curves. Nevertheless, these metrics are affected by the criteria of patient selection, the level of complexity of the procedure, and some other factors like institutional characteristics and experience of the team performing the procedure. As a result, one single value or an inflection point might fail to adequately capture the concept of surgical competence. The concepts of competency, proficiency, and mastery are distinct stages of learning, whereas the speed of learning for such skills as technical proficiency, clinical outcomes, oncological sufficiency, and intraoperative decision-making might be different. Conclusions: The learning curve in robotic HPB surgery should be viewed as a multi-dimensional and complex process and not a straightforward correlation between the number of cases and the results. The future assessment of the learning curve needs to consider different domains, including technical, clinical, oncological, cognitive, and team-related aspects, taking into account the level of complexity and institutional context of each case. FAU - Caringi, Silvio AU - Caringi S AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva delle Fonti, Italy. FAU - Delvecchio, Antonella AU - Delvecchio A AUID- ORCID: 0000-0002-7759-4340 AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva delle Fonti, Italy. AD - Department of Medicine and Surgery, LUM University, 70010 Casamassima, Italy. FAU - Casella, Annachiara AU - Casella A AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva delle Fonti, Italy. FAU - Ferraro, Valentina AU - Ferraro V AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva delle Fonti, Italy. FAU - Stasi, Matteo AU - Stasi M AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva delle Fonti, Italy. FAU - Tralli, Nunzio AU - Tralli N AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva delle Fonti, Italy. FAU - Manzia, Tommaso Maria AU - Manzia TM AUID- ORCID: 0000-0002-4636-3478 AD - Transplant and HPB Unit, Department of Surgery Sciences, University of Rome Tor Vergata, 00133 Rome, Italy. FAU - Inchingolo, Riccardo AU - Inchingolo R AUID- ORCID: 0000-0002-0253-5936 AD - Department of Medicine and Surgery, LUM University, 70010 Casamassima, Italy. AD - Unit of Interventional Radiology, "F. Miulli" General Hospital, 70021 Acquaviva delle Fonti, Italy. FAU - Tedeschi, Michele AU - Tedeschi M AUID- ORCID: 0000-0002-2101-5418 AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva delle Fonti, Italy. AD - Department of Medicine and Surgery, LUM University, 70010 Casamassima, Italy. FAU - Memeo, Riccardo AU - Memeo R AUID- ORCID: 0000-0002-1668-932X AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva delle Fonti, Italy. AD - Department of Medicine and Surgery, LUM University, 70010 Casamassima, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706305 OTO - NOTNLM OT - competency-based assessment OT - hepatopancreatobiliary surgery OT - learning curve OT - robotic surgery OT - surgical training COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:39 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - jcm15208043 [pii] AID - jcm-15-08043 [pii] AID - 10.3390/jcm15208043 [doi] PST - epublish SO - J Clin Med. 2026 Oct 16;15(20):8043. doi: 10.3390/jcm15208043. PMID- 42941543 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - A New Approach to Nipple-Sparing Mastectomy for Patients with Ptotic Breasts: A Single-Center, Retrospective Analysis of Feasibility and Treatment Outcomes. LID - 10.3390/jcm15208026 [doi] LID - 8026 AB - Background/Objectives: Subcutaneous mastectomies with immediate implant-based reconstruction have become widely performed procedures worldwide. Patients with small, non-ptotic breasts are candidates for procedures that do not involve skin reduction (NSM), whereas those with macromastia and significant ptosis undergo procedures involving the excision of excess skin (SRM). However, there remains a small group of patients for whom neither of these two options is ideal. We have developed a technique for reducing the implant pocket without excising excess skin, specifically designed for this group of patients. Methods: We conducted a retrospective analysis of 298 patients (419 breasts) who underwent surgery at our center between 2016 and 2025, comparing the method we described (NSM-LR) with NSM and SRM. A statistical analysis was performed. We evaluated oncological outcomes, complication rates, and implant loss. Satisfaction with the reconstructed breast was assessed using the Breast-Q questionnaire. Results: All cases were R0 resection. Locoregional recurrence was as follows: NSM-3.1%, NSM-LR-9.1%, and SRM-5.4% (p = 0.2363). Major complications were as follows: NSM-25.4%, NSM-LR-21.2%, and SRM-33.8% (p = 0.0887). Implant loss was as follows: NSM-7.6%, NSM-LR-10%, and SRM-17.6% (p = 0.0155). Breast Q score was as follows: NSM-78, NSM-LR-82, and SRM-75. Conclusions: NSM-LR can be a reasonable option for selected patients requiring a subcutaneous mastectomy. FAU - Pyka, Pawel AU - Pyka P AD - Clinical Department of Surgery, Wroclaw Medical University Branch Walbrzych, 50-367 Walbrzych, Poland. AD - Department of Surgical Oncology, Alfred Sokolowski's Specialistic Hospital in Walbrzych, 58-309 Walbrzych, Poland. FAU - Frejnagel, Andrzej AU - Frejnagel A AD - Department of Surgical Oncology, Alfred Sokolowski's Specialistic Hospital in Walbrzych, 58-309 Walbrzych, Poland. FAU - Karkotka, Dzmitry AU - Karkotka D AUID- ORCID: 0009-0004-8875-3089 AD - Department of Surgical Oncology, Alfred Sokolowski's Specialistic Hospital in Walbrzych, 58-309 Walbrzych, Poland. FAU - Nowak, Olga AU - Nowak O AD - Student Research Group SKN, Wroclaw Medical University, 50-367 Wroclaw, Poland. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705912 OTO - NOTNLM OT - breast cancer OT - breast implant OT - breast reconstruction OT - nipple-sparring mastectomy OT - skin-reducing mastectomy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:39 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - jcm15208026 [pii] AID - jcm-15-08026 [pii] AID - 10.3390/jcm15208026 [doi] PST - epublish SO - J Clin Med. 2026 Oct 16;15(20):8026. doi: 10.3390/jcm15208026. PMID- 42941534 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - Shoulder Injuries and Sport-Specific Shoulder Adaptations in Judo Athletes: A Systematic Review. LID - 10.3390/jcm15208020 [doi] LID - 8020 AB - Background/Objectives: The shoulder girdle is a frequent injury location in judo, and sport-specific shoulder-complex adaptations have been described in overhead sports, but these areas have not been synthesised for judo athletes. We synthesised evidence on (Q1) the frequency, anatomical profile, type and mechanism of shoulder girdle injuries and (Q2) the structural and functional characteristics of the shoulder complex in judokas. Methods: PubMed, Web of Science, Scopus and EBSCOhost were searched up to 7 August 2026. Two reviewers independently selected studies and extracted data. Risk of bias was assessed with JBI tools and ROBINS-I, and certainty of evidence with GRADE. Because injury definitions and measurement protocols were heterogeneous, a narrative synthesis (SWiM) with stratified descriptive analyses replaced the planned meta-analysis. Results: Twenty-three reports (22 studies) were included. Across 11 studies, shoulder girdle injuries accounted for 10.2-25.5% of recorded injuries (median 17.1%); this proportion depended on injury definition, exposure context and ascertainment and does not estimate prevalence. The shoulder ranked first or second among anatomical locations in 9 of 11 studies. Acromioclavicular joint injuries and glenohumeral dislocation/instability predominated. In one controlled study, judokas had higher internal rotator strength and a lower external-to-internal rotation (ER:IR) strength ratio than non-athletes; inter-limb asymmetry was inconsistent, and glenohumeral internal rotation deficit was not studied. Certainty of evidence was very low for all outcomes. Conclusions: The shoulder girdle is consistently among the most frequently injured locations in judo, whereas evidence for a judo-specific shoulder adaptation profile is sparse, heterogeneous and of very low certainty. FAU - Kasicki, Krzysztof AU - Kasicki K AUID- ORCID: 0009-0008-9231-8353 AD - Department of Sport Theory and Motor Skills, Institute of Sports Sciences, University of Physical Culture in Krakow, 31-571 Krakow, Poland. FAU - Blach, Wieslaw AU - Blach W AD - Faculty of Sport, Wroclaw University of Health and Sport Sciences, 51-612 Wroclaw, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706308 OTO - NOTNLM OT - adaptations OT - combat sports OT - glenohumeral joint OT - injury epidemiology OT - isokinetic strength OT - range of motion OT - shoulder complex OT - shoulder injuries COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:39 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - jcm15208020 [pii] AID - jcm-15-08020 [pii] AID - 10.3390/jcm15208020 [doi] PST - epublish SO - J Clin Med. 2026 Oct 16;15(20):8020. doi: 10.3390/jcm15208020. PMID- 42941531 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - Quantitative CT Geometry of the Patent Ductus Arteriosus in Infants with Congenital Heart Disease: Association with Aortic Arch Obstruction or Hypoplasia. LID - 10.3390/jcm15208017 [doi] LID - 8017 AB - Background/Objectives: Quantitative patent ductus arteriosus (PDA) geometry in congenital heart disease (CHD), particularly in relation to aortic arch abnormalities, is incompletely characterized. We evaluated CT-derived PDA geometry. Methods: This retrospective exploratory single-center study included 168 infants with CHD and a PDA on cardiovascular CT. Aortic-end, pulmonary-end, and mid-duct diameters and ductal length were measured. The Ductal Length-to-Diameter Index (LDI), selected during exploratory analysis, was defined as ductal length divided by the mean end diameter. Seventy-three patients had CT-documented aortic arch obstruction or hypoplasia. Results: LDI was lower in patients with arch obstruction/hypoplasia than in those without these findings (2.04 [1.39-2.84] vs. 3.51 [2.23-4.89]; p < 0.001; rank-biserial r = -0.473) and remained associated after adjustment for age, weight, and sex (OR per +1 LDI, 0.534; 95% CI, 0.412-0.693; p < 0.001). The association remained robust with a conservative definition restricted to coarctation or interrupted aortic arch. LDI had an interobserver ICC of 0.843 (95% CI, 0.685-0.954), with wide limits of agreement (-1.98 to +2.45). Conclusions: CT-documented aortic arch obstruction or hypoplasia is associated with lower LDI. This exploratory geometric association warrants prospective external validation before clinical use. FAU - Pop, Marian AU - Pop M AUID- ORCID: 0000-0001-5324-9780 AD - Faculty of Medicine in English, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142 Targu Mures, Romania. AD - Radiology and Medical Imaging Laboratory, Mures County Emergency Clinical Hospital, 540136 Targu Mures, Romania. AD - Emergency Institute for Cardiovascular Diseases and Transplantation of Targu Mures, 540139 Targu Mures, Romania. FAU - Bargutin, Liel AU - Bargutin L AD - Faculty of Medicine in English, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142 Targu Mures, Romania. FAU - Licu, Razvan-Andrei AU - Licu RA AUID- ORCID: 0009-0007-5406-1449 AD - Radiology and Medical Imaging Laboratory, Mures County Emergency Clinical Hospital, 540136 Targu Mures, Romania. AD - Doctoral School of Medicine and Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142 Targu Mures, Romania. FAU - Rotariu, Marian AU - Rotariu M AD - Radiology and Medical Imaging Laboratory, Mures County Emergency Clinical Hospital, 540136 Targu Mures, Romania. FAU - Iurian, Diana-Ramona AU - Iurian DR AD - Radiology and Medical Imaging Laboratory, Mures County Emergency Clinical Hospital, 540136 Targu Mures, Romania. AD - Doctoral School of Medicine and Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142 Targu Mures, Romania. FAU - Negovan, Tania AU - Negovan T AD - Radiology and Medical Imaging Laboratory, Mures County Emergency Clinical Hospital, 540136 Targu Mures, Romania. AD - Doctoral School of Medicine and Pharmacy, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142 Targu Mures, Romania. FAU - Suciu, Horatiu AU - Suciu H AD - Emergency Institute for Cardiovascular Diseases and Transplantation of Targu Mures, 540139 Targu Mures, Romania. AD - Department of Surgery IV, George Emil Palade University of Medicine, Pharmacy, Science and Technology of Targu Mures, 540142 Targu Mures, Romania. AD - Clinic of Cardiovascular Surgery, Emergency Institute for Cardiovascular Diseases and Transplantation of Targu Mures, 540139 Targu Mures, Romania. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706272 OTO - NOTNLM OT - aortic arch hypoplasia OT - aortic coarctation OT - computed tomography angiography OT - congenital heart disease OT - ductal length-to-diameter index OT - morphometry OT - patent ductus arteriosus COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:39 PHST- 2026/08/28 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - jcm15208017 [pii] AID - jcm-15-08017 [pii] AID - 10.3390/jcm15208017 [doi] PST - epublish SO - J Clin Med. 2026 Oct 16;15(20):8017. doi: 10.3390/jcm15208017. PMID- 42941530 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - Layer-Oriented Lateral Superior Approach for Robotic Right Hemicolectomy: A Novel Embryology-Based Strategy and Initial Clinical Outcomes. LID - 10.3390/jcm15208016 [doi] LID - 8016 AB - Background: Complete mesocolic excision (CME) with D3 lymphadenectomy is an oncologically established procedure for right-sided colon cancer; however, the optimal dissection plane and surgical approach remain controversial. We developed a novel layer-oriented lateral superior approach (LSA) that enables the intentional, depth-tailored selection of embryological dissection planes in robotic right hemicolectomy. Methods: This retrospective single-center study included consecutive patients who underwent robotic right hemicolectomy using the LSA between June 2023 and May 2025. The dissection plane was selected according to tumor depth (/=T3). Perioperative and short-term oncological outcomes were evaluated. Results: Thirty-six patients were included. The median operative time was 289 min, and the median blood loss was 10 mL. No intraoperative complications or conversions occurred. Postoperative complications were observed in 6 patients (16.7%), all /=40 Years: A Hierarchical Analysis of Comorbidity-Associated Patterns. LID - 10.3390/jcm15208013 [doi] LID - 8013 AB - Background/Objectives: Selecting appropriate analgesic therapy in patients with multimorbidity is increasingly challenging because clinicians must balance pain management against comorbidities, polypharmacy, and medication-related risks. However, evidence describing how analgesic prescribing patterns vary across patients with different multimorbidity profiles in routine outpatient practice remains limited. This retrospective cross-sectional study examined associations between multimorbidity and prescribing patterns across paracetamol, non-steroidal anti-inflammatory drugs (NSAIDs), and opioids, and identified clinical characteristics associated with different hierarchical analgesic exposure groups among adults aged >/=40 years in Saudi Arabia. Methods: Electronic health record data were analyzed for adults aged >/=40 years attending multiple clinics in the Eastern Region of Saudi Arabia between January 2020 and December 2021. Analgesic exposure at the index outpatient visit was classified using a hierarchical framework into mutually exclusive paracetamol-only, NSAID-priority, opioid-priority, or non-use groups. Multinomial logistic regression was performed to identify clinical characteristics associated with assignment to the hierarchical analgesic exposure groups. Results: Among 41,083 patients, 29,383 (71.5%) received at least one analgesic prescription at the index visit. The NSAID-priority group was the largest exposure category, followed by opioid-priority and paracetamol-only groups. Hierarchical analgesic prescribing patterns differed across patient clinical profiles, with prescribing observed across patients with cardiovascular, metabolic, gastrointestinal, respiratory, and neuropsychiatric disorders. In adjusted analyses, arthritis-related disease was associated with slightly higher odds of NSAID-priority assignment, whereas ischemic heart disease, gastritis-related disease, and respiratory disorders were associated with lower odds of NSAID-priority assignment. Constipation and urinary incontinence were associated with lower odds of opioid-priority assignment. Conclusions: Analgesic prescribing was common among medically complex adult outpatients aged >/=40 years, and hierarchical prescribing patterns differed across patient clinical and comorbidity profiles. The hierarchical framework provided a pragmatic approach for characterizing prescribing across major analgesic classes while accounting for concurrent exposure. Most disease-specific associations were attenuated after multivariable adjustment, and the remaining associations were generally modest in magnitude. Owing to the cross-sectional design and absence of detailed treatment-indication and pain-related information, these findings represent prescribing associations and should not be interpreted as evidence of causal prescribing decisions or treatment appropriateness. FAU - Alqurain, Aymen Ali AU - Alqurain AA AUID- ORCID: 0000-0002-4284-0265 AD - Department of Clinical Practice, Faculty of Pharmacy, Northern Border University, Rafha 91911, Saudi Arabia. FAU - Alshammari, Abdulkarim AU - Alshammari A AUID- ORCID: 0009-0008-6318-4341 AD - Department of Clinical Practice, Faculty of Pharmacy, Northern Border University, Rafha 91911, Saudi Arabia. FAU - Alhomoud, Faten AU - Alhomoud F AUID- ORCID: 0000-0002-6638-1390 AD - Department of Pharmacy Practice, College of Pharmacy, Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia. FAU - Alhomoud, Farah Kais AU - Alhomoud FK AD - Department of Pharmacy Practice, College of Pharmacy, Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia. FAU - Alsalman, Abdulkhaliq J AU - Alsalman AJ AD - Department of Clinical Practice, Faculty of Pharmacy, Northern Border University, Rafha 91911, Saudi Arabia. FAU - Alshammari, Mohammad Kanan AU - Alshammari MK AUID- ORCID: 0000-0001-6687-0461 AD - Department of Pharmaceutical Care, Northern Borders Cluster, Rafha Centeral Hospital, Rafha 76321, Saudi Arabia. FAU - Gharash, Hassan Al AU - Gharash HA AUID- ORCID: 0000-0003-3728-4839 AD - Emergency and Intensive Care Department, College of Nursing, Northern Border University, Arar 73213, Saudi Arabia. FAU - Alshawaf, Abdullah Jawad AU - Alshawaf AJ AD - Department of Respiratory Therapy, Mohammed Al-Mana College for Medical Sciences, Dammam 34222, Saudi Arabia. LA - eng GR - NBU-FFR2026-396-03/Northern Border University/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705678 OTO - NOTNLM OT - analgesic exposure OT - analgesic prescribing OT - multimorbidity OT - outpatients OT - pharmacoepidemiology OT - polypharmacy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:39 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - jcm15208013 [pii] AID - jcm-15-08013 [pii] AID - 10.3390/jcm15208013 [doi] PST - epublish SO - J Clin Med. 2026 Oct 16;15(20):8013. doi: 10.3390/jcm15208013. PMID- 42941525 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Caregiver Burden in an Online Sample of Family Caregivers: Cross-Sectional Associations with Anticipatory Grief and Family Needs. LID - 10.3390/jcm15208011 [doi] LID - 8011 AB - Background/Objectives: Caregiver burden is common among family caregivers providing home-based care and may be associated with anticipatory grief (i.e., the emotional response to an expected loss before death), family-needs satisfaction, and care-related factors. This study aimed to assess the proportion of caregivers reporting burden and examine the independent and incremental associations of burden with characteristics of the caregiver, family, and care recipient in a Portuguese sample. Methods: An online cross-sectional, observational, descriptive-correlational study was conducted with 401 family caregivers of individuals with perceived PC needs in Portugal. Family caregivers were recruited through convenience and snowball sampling using healthcare networks, caregiver organizations, and social media platforms. Data were collected through an e-survey comprising sociodemographic and clinical variables, the Zarit Burden Interview (ZBI), the Family Inventory of Needs (FIN), and the Marwit-Meuser Caregiver Grief Inventory-Short Form (MM-CGI-SF). Descriptive statistics, bivariate analyses, and hierarchical multiple linear regression were performed to identify factors associated with caregiver burden. Missing data were handled using complete-case analysis. The study followed the STROBE and CHERRIES guidelines. Results: The mean age of caregivers was 55.93 +/- 13.30 years, and most participants were female (81.5%). The mean ZBI total score was 57.91 +/- 15.23. Using the predefined threshold (ZBI >/= 46), 77.1% of respondents met criteria for caregiver burden. This proportion comprised 25.2% with mild burden and 51.9% with severe burden. Caregivers experiencing burden reported significantly higher anticipatory grief, lower satisfaction with family needs, and greater patient symptom burden. Hierarchical multiple linear regression explained 56.4% of the variance in caregiver burden (adjusted R(2) = 0.541). Psychosocial factors accounted for the largest incremental contribution to explained variance (DeltaR(2) = 0.490, p < 0.001). In the fully adjusted model, anticipatory grief showed the strongest independent positive association with caregiver burden (beta = 0.622, p < 0.001), whereas greater fulfilment of care needs was independently associated with lower burden (beta = -0.180, p < 0.001). Conclusions: Findings should be interpreted as cross-sectional associations within a self-selected online sample. Caregiver burden was common among family caregivers providing home-based care and was associated with higher anticipatory grief and lower family-needs satisfaction. Routine assessment and family-centred support interventions may help address caregivers' needs in this context. FAU - Ramos, Ana AU - Ramos A AD - School of Health Sciences, University of Leiria and Oeste, Rua General Norton de Matos, 2411-901 Leiria, Portugal. FAU - Reami, Gisele AU - Reami G AD - School of Health Sciences, University of Leiria and Oeste, Rua General Norton de Matos, 2411-901 Leiria, Portugal. FAU - Querido, Ana AU - Querido A AUID- ORCID: 0000-0002-5021-773X AD - School of Health Sciences, University of Leiria and Oeste, Rua General Norton de Matos, 2411-901 Leiria, Portugal. AD - Centre for Innovative Care and Health Technology (ciTechCare), University of Leiria and Oeste, Campus 5, Rua das Olhalvas, 2414-016 Leiria, Portugal. AD - RISE Health Research Group, University of Porto, 4200-450 Porto, Portugal. FAU - Dixe, Maria Dos Anjos AU - Dixe MDA AUID- ORCID: 0000-0001-9035-8548 AD - School of Health Sciences, University of Leiria and Oeste, Rua General Norton de Matos, 2411-901 Leiria, Portugal. AD - Centre for Innovative Care and Health Technology (ciTechCare), University of Leiria and Oeste, Campus 5, Rua das Olhalvas, 2414-016 Leiria, Portugal. FAU - Laranjeira, Carlos AU - Laranjeira C AUID- ORCID: 0000-0003-1080-9535 AD - School of Health Sciences, University of Leiria and Oeste, Rua General Norton de Matos, 2411-901 Leiria, Portugal. AD - Centre for Innovative Care and Health Technology (ciTechCare), University of Leiria and Oeste, Campus 5, Rua das Olhalvas, 2414-016 Leiria, Portugal. AD - Comprehensive Health Research Centre (CHRC), University of Evora, 7000-801 Evora, Portugal. LA - eng GR - 2023.15123.PEX "ALL ABOARD: Access family caregiving needs as a bridge for palliative care-in-place"/Fundacao para a Ciencia e Tecnologia/ GR - https://doi.org/10.54499/UID/05704/2025/Fundacao para a Ciencia e Tecnologia/ GR - https://doi.org/10.54499/CEECINST/00051/2018/CP1566/CT0012/Fundacao para a Ciencia e Tecnologia/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706454 OTO - NOTNLM OT - anticipatory grief OT - caregiver burden OT - family caregivers OT - family needs OT - home care OT - palliative care COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:39 PHST- 2026/08/05 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - jcm15208011 [pii] AID - jcm-15-08011 [pii] AID - 10.3390/jcm15208011 [doi] PST - epublish SO - J Clin Med. 2026 Oct 15;15(20):8011. doi: 10.3390/jcm15208011. PMID- 42941522 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Weight-Loss Magnitude, Fat Mass, and Device-Estimated Muscle Mass Loss During Obesity Pharmacotherapy. LID - 10.3390/jcm15208008 [doi] LID - 8008 AB - Background: Body-composition changes during routine obesity pharmacotherapy require cautious interpretation. Methods: This retrospective observational cohort included 59 adults with paired InBody 770 assessments: 53 receiving tirzepatide and 6 receiving semaglutide. Paired changes and relative device-estimated muscle mass loss were analyzed in all 59; component-share analyses were restricted to the 51 achieving >/=5% weight loss. Analyses used rank-based methods and exploratory robust regression. Results: Recorded treatment duration was a median of 7.0 months, and median observed weight loss was 12.11%. Median fat mass and device-estimated muscle mass losses were 9.9 and 2.3 kg, respectively. Among the 51 participants achieving >/=5% weight loss, median fat, device-estimated muscle, and residual shares were 73.95%, 14.91%, and 10.85%. Each additional five percentage points of weight loss was associated with 2.47 percentage points greater relative device-estimated muscle mass loss (95% CI, 1.89 to 3.05). The corresponding fully adjusted association with muscle share was -0.52 percentage points (95% CI, -1.66 to 0.62); this does not establish stability or equivalence. Final drug-specific dose intensity was not statistically associated with either outcome in the adjusted models. Conclusions: Weight loss observed in this selected, predominantly tirzepatide-treated cohort was mainly accounted for by estimated fat mass reduction. Larger observed weight-loss responses accompanied greater device-estimated muscle mass reductions. Muscle-share estimates were uncertain and model-dependent. The study cannot establish causal medication effects or functional consequences. FAU - Tomescu, Cristina AU - Tomescu C AD - Doctoral School of Medicine, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. AD - Centre for Molecular Research in Nephrology and Vascular Disease, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. FAU - Albai, Alin AU - Albai A AD - Centre for Molecular Research in Nephrology and Vascular Disease, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. AD - Second Department of Internal Medicine, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. FAU - Avram, Vlad Florian AU - Avram VF AD - Centre for Molecular Research in Nephrology and Vascular Disease, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. AD - Second Department of Internal Medicine, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. FAU - Timar, Sandra AU - Timar S AD - First Department of Internal Medicine, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. AD - Multidisciplinary Research Center for Malignant Hematological Diseases (CCMHM), "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. FAU - Cipu, Anamaria AU - Cipu A AD - Doctoral School of Medicine, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. AD - Centre for Molecular Research in Nephrology and Vascular Disease, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. FAU - Timar, Bogdan AU - Timar B AUID- ORCID: 0000-0002-0993-6206 AD - Centre for Molecular Research in Nephrology and Vascular Disease, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. AD - Second Department of Internal Medicine, "Victor Babes" University of Medicine and Pharmacy, 300041 Timisoara, Romania. LA - eng GR - Victor Babes University of Medicine and Pharmacy Timisoara/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706178 OTO - NOTNLM OT - bioelectrical impedance OT - device-estimated muscle mass OT - fat mass OT - obesity pharmacotherapy OT - weight loss COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:39 PHST- 2026/09/20 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - jcm15208008 [pii] AID - jcm-15-08008 [pii] AID - 10.3390/jcm15208008 [doi] PST - epublish SO - J Clin Med. 2026 Oct 15;15(20):8008. doi: 10.3390/jcm15208008. PMID- 42941520 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Reduction of Arterial Stiffness Assessed by Cardio-Ankle Vascular Index Through Intra-Dialytic Aerobic Training. LID - 10.3390/jcm15208005 [doi] LID - 8005 AB - Background: Increased arterial stiffness elevates mortality in chronic kidney disease. Limited available data suggest that regular exercise can reduce arterial stiffness in hemodialysis patients. This pilot study aimed to investigate the effects of intra-dialytic aerobic training on arterial stiffness in hemodialysis patients. Methods: Outpatients undergoing hemodialysis were randomized to a 20-week intra-dialytic aerobic training group (bedside ergometer exercise) or a control group receiving standard care. Arterial stiffness was evaluated using the cardio-ankle vascular index (CAVI). CAVI measurements, functional fitness tests, laboratory analyses, and quality-of-life assessments were performed at baseline and at the end of the study. Results: A significant decrease in CAVI was observed in the intra-dialytic aerobic training group (-5%; p = 0.037). Concurrently, significant improvements occurred in VO2peak/kg (+31%; p = 0.015), 6 min walk test (6 MWT) distance (+18%; p = 0.005), clearance (URR +2%; p < 0.001; Kt/V +6%; p = 0.001), and select quality-of-life parameters (social support +9%; p = 0.022; general health +25%; p = 0.008; well-being +3%; p < 0.001). No significant changes were found in the control group. Conclusions: The reduction in arterial stiffness confirms the efficacy of intra-dialytic aerobic training in improving overall health status. Consequently, CAVI appears to be a valuable tool for monitoring arterial stiffness changes in hemodialysis patients. FAU - Havelkova, Alena AU - Havelkova A AD - Department of Sports Medicine and Rehabilitation, St. Anne's University Hospital in Brno, Faculty of Medicine, Masaryk University, 602 00 Brno, Czech Republic. AD - Department of Physical Activities and Health Sciences, Faculty of Sports Studies, Masaryk University, 625 00 Brno, Czech Republic. FAU - Dobsak, Petr AU - Dobsak P AUID- ORCID: 0000-0002-7290-6165 AD - Department of Sports Medicine and Rehabilitation, St. Anne's University Hospital in Brno, Faculty of Medicine, Masaryk University, 602 00 Brno, Czech Republic. FAU - Dvorak, Petr AU - Dvorak P AD - Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences, 612 42 Brno, Czech Republic. FAU - Pohanka, Michal AU - Pohanka M AD - Department of Sports Medicine and Rehabilitation, St. Anne's University Hospital in Brno, Faculty of Medicine, Masaryk University, 602 00 Brno, Czech Republic. FAU - Pokorna, Alena AU - Pokorna A AUID- ORCID: 0009-0009-5900-3058 AD - Department of Sports Medicine and Rehabilitation, St. Anne's University Hospital in Brno, Faculty of Medicine, Masaryk University, 602 00 Brno, Czech Republic. FAU - Novakova, Marie AU - Novakova M AUID- ORCID: 0000-0003-3020-4250 AD - Department of Physiology, Faculty of Medicine, Masaryk University, 625 00 Brno, Czech Republic. FAU - Filipensky, Petr AU - Filipensky P AUID- ORCID: 0000-0003-2383-4414 AD - Department of Urology, St. Anne's University Hospital in Brno, Faculty of Medicine, Masaryk University, 602 00 Brno, Czech Republic. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706399 OTO - NOTNLM OT - arterial stiffness OT - cardio-ankle vascular index OT - cardioprotective effect OT - functional capacity OT - hemodialysis OT - intradialytic exercise COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:39 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - jcm15208005 [pii] AID - jcm-15-08005 [pii] AID - 10.3390/jcm15208005 [doi] PST - epublish SO - J Clin Med. 2026 Oct 15;15(20):8005. doi: 10.3390/jcm15208005. PMID- 42941519 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Management of Symptomatic Genu Recurvatum: Biomechanical Rationale, Etiology-Based Treatment Algorithm, and a Surgical Technical Blueprint. LID - 10.3390/jcm15208006 [doi] LID - 8006 AB - Symptomatic genu recurvatum is an uncommon sagittal-plane knee deformity associated with pathological hyperextension, pain, instability, gait disturbance, and functional limitation. Its heterogeneous causes and limited clinical evidence complicate treatment selection. This narrative review summarizes current concepts in biomechanics, etiologic classification, diagnostic evaluation, and management, with emphasis on posterior tibial slope (PTS) and slope-correcting proximal tibial osteotomy. Clinically relevant literature on genu recurvatum, sagittal knee alignment, osteotomy, and ligamentous instability was integrated with an illustrative previously reported case to provide a surgical technical blueprint. The available evidence supports an etiology-based approach. Reduced or reversed PTS is an important osseous contributor to pathological hyperextension, whereas soft-tissue, neuromuscular, and combined deformities require different strategies. In selected patients with osseous recurvatum and intact ligamentous restraints, anterior opening-wedge proximal tibial osteotomy can restore posterior slope and sagittal alignment. Critical technical steps include reproducible radiographic planning, fluoroscopically controlled K-wire placement, progressive posterior osteotomy completion, preservation of the posterior cortical hinge, gradual wedge opening, and stable fixation. An etiology-based diagnostic and treatment algorithm may facilitate individualized decision-making while avoiding unnecessary or incomplete procedures. FAU - Stevanovic, Vladan AU - Stevanovic V AUID- ORCID: 0000-0003-3843-152X AD - Institute for Orthopedics "Banjica", 11040 Belgrade, Serbia. AD - Faculty of Medicine, University of Belgrade, 11000 Belgrade, Serbia. FAU - Cucakovic, Filip AU - Cucakovic F AUID- ORCID: 0009-0006-3298-3349 AD - Institute for Orthopedics "Banjica", 11040 Belgrade, Serbia. LA - eng GR - This research was funded by the Ministry of Science, Technological Development and Innovation of the Republic of Serbia through the Grant Agreement with the University of Belgrade-Faculty of Medicine, No. 451-03-34/2026-03/200110./University of Belgrade - Faculty of Medicine/ PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705637 OTO - NOTNLM OT - genu recurvatum OT - knee instability OT - osteotomy OT - posterior tibial slope OT - proximal tibial osteotomy OT - sagittal alignment OT - surgical technique COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:39 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - jcm15208006 [pii] AID - jcm-15-08006 [pii] AID - 10.3390/jcm15208006 [doi] PST - epublish SO - J Clin Med. 2026 Oct 15;15(20):8006. doi: 10.3390/jcm15208006. PMID- 42941517 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Real-World Sacubitril/Valsartan Adoption and Post-Initiation Dose Reassessment and Titration in Heart Failure with Reduced Ejection Fraction: An Explanatory Sequential Mixed-Methods Study from Northern Saudi Arabia. LID - 10.3390/jcm15208001 [doi] LID - 8001 AB - Background/Objectives: Sacubitril/valsartan is a foundational therapy for heart failure with reduced ejection fraction (HFrEF), but real-world implementation extends beyond initiation to laboratory monitoring, follow-up, dose reassessment, and documentation of reasons for sub-target therapy. We evaluated this prescribing pathway in a regional Saudi heart-failure service. Methods: This explanatory sequential mixed-methods study included 296 adults with HFrEF (left ventricular ejection fraction /=6 months of follow-up, 75.1% completed renal-function/potassium reassessment within 14 days, 67.9% had follow-up within six weeks, and 60.6% had an escalation attempt within 90 days. Current target-dose use was 16.7%, and 21.7% had reached target at any point. Of 116 patients currently receiving 24/26 mg twice daily, 91 had never been documented above the lowest dose, and 25 had previously reached a higher dose. Reasons for current lowest-dose therapy were clinical/physiological in 49, health-system/access-related in 5, patient preference/adherence-related in 4, and undocumented in 58. Qualitative findings emphasized monitoring continuity, explicit role ownership, appointment access, dose availability, and communication. Conclusions: High ARNI uptake did not ensure complete post-initiation dose reassessment and titration. The clearest measurable gap was uncertainty around current lowest-dose therapy when no reason or reassessment plan was documented; this represents a documentation/reassessment gap rather than proof of inappropriate care. Clinical audit should distinguish target-dose attainment, clinically limited sub-target dosing, active titration, return to a lower dose after prior escalation, access-related barriers, patient preference/adherence, and unresolved sub-target therapy. FAU - Alonayzan, Reham Olayan AU - Alonayzan RO AD - Department of Biopharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19328, Jordan. FAU - Alsa'd, Alhareth AU - Alsa'd A AUID- ORCID: 0009-0006-0395-7491 AD - Department of Biopharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19328, Jordan. FAU - Zakaraya, Zainab AU - Zakaraya Z AUID- ORCID: 0000-0003-3691-9759 AD - Department of Biopharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19328, Jordan. FAU - Malouh, Marwa A AU - Malouh MA AD - Department of Biopharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19328, Jordan. FAU - Abed, Anas AU - Abed A AUID- ORCID: 0000-0003-2828-1011 AD - Department of Biopharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman 19328, Jordan. FAU - AlRasheed, Hayam A AU - AlRasheed HA AUID- ORCID: 0009-0002-6186-848X AD - Department of Pharmacy Practice, College of Pharmacy, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia. LA - eng GR - PNURSP2026R485/Princess Nourah bint Abdulrahman University/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705730 OTO - NOTNLM OT - HFrEF OT - Saudi Arabia OT - angiotensin receptor-neprilysin inhibitor OT - dose titration OT - guideline-directed medical therapy OT - implementation science OT - mixed methods OT - multidisciplinary care OT - prescribing quality OT - sacubitril/valsartan COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:39 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - jcm15208001 [pii] AID - jcm-15-08001 [pii] AID - 10.3390/jcm15208001 [doi] PST - epublish SO - J Clin Med. 2026 Oct 15;15(20):8001. doi: 10.3390/jcm15208001. PMID- 42941516 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Liver Function Assessment in Oncology: Current Tools for Evaluating Hepatic Reserve and Surgical Decision-Making. LID - 10.3390/jcm15208003 [doi] LID - 8003 AB - Accurate assessment of hepatic functional reserve is essential in patients with primary or secondary liver malignancies undergoing liver resection, particularly in the presence of chronic liver disease, chemotherapy-associated liver injury, cholestasis, or portal hypertension. Conventional laboratory parameters and clinical scores, including Child-Pugh, MELD, ALBI, and the combined APRI/ALBI score, provide accessible measures of global hepatic status and risk stratification but do not directly quantify regional function or dynamic metabolic capacity. Complementary approaches include indocyanine green clearance, LiMAx testing, liver stiffness measurement, hepatobiliary scintigraphy, gadoxetic acid-enhanced magnetic resonance imaging, and CT/MRI-based future liver remnant volumetry. This narrative review critically examines the strengths, limitations, and clinical applicability of these modalities in the context of contemporary evidence and recommendations. Building on, but extending beyond, the 2023 E-AHPBA-ESSO-ESSR Innsbruck consensus, we incorporate subsequent comparative evidence on blood-based scores and functional imaging and discuss emerging approaches including longitudinal assessment, radiomics, and artificial intelligence. Current evidence supports an individualized, multimodal strategy in which volumetry remains central to surgical planning, while additional functional and portal-hypertension assessment is selected according to the extent of resection, underlying parenchymal disease, previous treatment, and local expertise. No single modality or fixed combination can currently be considered universally superior, highlighting the need for prospective validation of integrated and cost-effective assessment strategies. FAU - Caringi, Silvio AU - Caringi S AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva delle Fonti, Italy. FAU - Delvecchio, Antonella AU - Delvecchio A AUID- ORCID: 0000-0002-7759-4340 AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva delle Fonti, Italy. AD - Department of Medicine and Surgery, LUM University, 70010 Casamassima, Italy. FAU - Casella, Annachiara AU - Casella A AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva delle Fonti, Italy. FAU - Ferraro, Valentina AU - Ferraro V AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva delle Fonti, Italy. FAU - Stasi, Matteo AU - Stasi M AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva delle Fonti, Italy. FAU - Tralli, Nunzio AU - Tralli N AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva delle Fonti, Italy. FAU - Manzia, Tommaso Maria AU - Manzia TM AUID- ORCID: 0000-0002-4636-3478 AD - Transplant and HPB Unit, Department of Surgery Sciences, University of Rome Tor Vergata, 00133 Rome, Italy. FAU - Inchingolo, Riccardo AU - Inchingolo R AUID- ORCID: 0000-0002-0253-5936 AD - Unit of Interventional Radiology, "F. Miulli" General Hospital, 70021 Acquaviva delle Fonti, Italy. FAU - Tedeschi, Michele AU - Tedeschi M AUID- ORCID: 0000-0002-2101-5418 AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva delle Fonti, Italy. AD - Department of Medicine and Surgery, LUM University, 70010 Casamassima, Italy. FAU - Memeo, Riccardo AU - Memeo R AUID- ORCID: 0000-0002-1668-932X AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva delle Fonti, Italy. AD - Department of Medicine and Surgery, LUM University, 70010 Casamassima, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706092 OTO - NOTNLM OT - functional liver imaging OT - future liver remnant OT - hepatic functional reserve OT - hepatobiliary scintigraphy OT - liver function OT - liver resection OT - portal hypertension OT - post-hepatectomy liver failure COIS- Drs. Annachiara Casella, Antonella Delvecchio, Matteo Stasi, Michele Tedeschi, Nunzio Tralli. Riccardo Inchingolo, Riccardo Memeo, Silvio Caringi, Tommaso Maria Manzia and Valentina Ferraro have no conflicts of interest or financial ties to disclose. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:39 PHST- 2026/08/14 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - jcm15208003 [pii] AID - jcm-15-08003 [pii] AID - 10.3390/jcm15208003 [doi] PST - epublish SO - J Clin Med. 2026 Oct 15;15(20):8003. doi: 10.3390/jcm15208003. PMID- 42941515 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Early Anesthesia-Period Stress Hyperglycemia Ratio and In-Hospital Mortality After Noncardiac Surgery in Patients with HbA1c and Glucose Measurements. LID - 10.3390/jcm15208002 [doi] LID - 8002 AB - Background/Objectives: We examined the association between early anesthesia-period relative hyperglycemia and in-hospital mortality after noncardiac surgery. Methods: This retrospective INSPIRE cohort included admissions with pre-anesthesia HbA1c and glucose measured within 60 min after anesthesia start. Early iSHR was first glucose divided by HbA1c-derived estimated average glucose; whole-anesthesia peak iSHR was secondary. Follow-up extended from anesthesia end until death or same-admission discharge, with variable duration. Logistic spline and linear models used patient-clustered standard errors. Results: Of 117,429 source admissions, 10,406 were included, with 101 deaths. The core-adjusted OR per 0.10 increase in early iSHR was 1.134 (95% CI 1.063-1.210). Further adjustment for measurement count, renal function, malignancy, hypotension, and blood-product records yielded an OR of 1.089 (1.014-1.170) in 10,352 admissions with 97 deaths. For whole-anesthesia peak iSHR (11,374 admissions; 104 deaths), otherwise matched models without and with low-MAP proportion and blood-product records yielded ORs of 1.126 (1.063-1.193) and 1.064 (0.999-1.133; p = 0.052), respectively. A separate TWA/count model yielded an OR of 1.039 (0.973-1.109) in 11,312 admissions. Internal comparisons showed no clear discrimination advantage over absolute early glucose. Conclusions: In clinically selected patients with pre-anesthesia HbA1c and early glucose measurements, early iSHR was associated with in-hospital mortality in core and further-adjusted models, although creatinine-complete and influence analyses indicated limited robustness. The whole-anesthesia peak-iSHR estimate attenuated and showed no clear association when hypotension and blood-product records were included; the TWA/count model also showed no clear association. Internal comparison did not establish a predictive advantage over absolute early glucose; causality cannot be inferred. FAU - Zhang, Jianhao AU - Zhang J AUID- ORCID: 0009-0009-8198-5625 AD - Department of Anesthesiology, Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 33 Badachu Road, Shijingshan District, Beijing 100144, China. FAU - Zhang, Yuguan AU - Zhang Y AUID- ORCID: 0000-0003-4149-8370 AD - Department of Anesthesiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 1 Shuaifuyuan, Wangfujing, Dongcheng District, Beijing 100730, China. FAU - Wei, Lingxin AU - Wei L AD - Department of Anesthesiology, Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 33 Badachu Road, Shijingshan District, Beijing 100144, China. LA - eng GR - YS2024CG022/Plastic Surgery Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706281 OTO - NOTNLM OT - INSPIRE OT - anesthesia OT - in-hospital mortality OT - noncardiac surgery OT - perioperative hyperglycemia OT - stress hyperglycemia ratio COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:39 PHST- 2026/08/29 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - jcm15208002 [pii] AID - jcm-15-08002 [pii] AID - 10.3390/jcm15208002 [doi] PST - epublish SO - J Clin Med. 2026 Oct 15;15(20):8002. doi: 10.3390/jcm15208002. PMID- 42941510 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Assessment of Liver Fibrosis Using the Coefficient of Variation on Gadoxetic Acid-Enhanced Hepatobiliary Phase MR Imaging: A Prospective Multicenter Study. LID - 10.3390/jcm15207996 [doi] LID - 7996 AB - Background: Accurate non-invasive assessment of liver fibrosis is needed in patients with chronic liver disease. Gadoxetic acid (Gd-EOB-DTPA)-enhanced hepatobiliary phase (HBP) magnetic resonance (MR) imaging reflects hepatocyte function and parenchymal heterogeneity, and the coefficient of variation (CV), which quantifies pixel-level signal inhomogeneity, has been proposed as a quantitative imaging biomarker of liver fibrosis. However, previous studies were retrospective, single-center, and lacked direct pathological validation. Objectives: To investigate the factors associated with CV on HBP images, evaluate the correlation between CV and pathologically confirmed liver fibrosis stage, and compare the diagnostic performance of CV with serum biomarkers, including the aspartate aminotransferase-to-platelet ratio index (APRI) and fibrosis-4 (FIB-4), in a prospective multicenter setting. Methods: This prospective multicenter study was conducted between April 2017 and August 2020 at three medical centers. Ninety-nine participants who underwent Gd-EOB-DTPA-enhanced HBP T1-weighted MRI and liver biopsy or surgery were enrolled. CV was measured on HBP images, and the METAVIR fibrosis stage was used as the reference standard. Univariate and multivariable linear regression analyses, including an additional model adjusted for institution and pathology confirmation method, Welch's analysis of variance with the Games-Howell post hoc test, Spearman's rank correlation analysis, receiver operating characteristic (ROC) curve analysis with DeLong's method for AUROC comparison, and intraclass correlation coefficient (ICC) analyses were performed. Results: Ninety-nine participants (64 men and 35 women; mean age, 55.5 +/- 15.1 years) were enrolled. In multivariable linear regression analysis adjusted for institution and pathology confirmation method, pathologic fibrosis stage (B = 0.468; 95% CI, 0.358-0.578; p < 0.001) and serum albumin level (B = -0.341; p = 0.015) were independently associated with CV. Spearman's correlation coefficient between CV and fibrosis stage was 0.74 (p < 0.001). The AUROCs of CV for significant fibrosis (>/=F2), advanced fibrosis (>/=F3), and cirrhosis (F4) were 0.92, 0.88, and 0.86, respectively (all p < 0.001). CV outperformed APRI and FIB-4 across most fibrosis thresholds (p < 0.001 to p = 0.045), except for FIB-4 in the diagnosis of >/=F2 fibrosis (p = 0.09). The intra- and inter-observer ICC values were 0.94 and 0.84, respectively. Conclusions: In this prospective multicenter study with pathologically confirmed fibrosis stages, CV derived from Gd-EOB-DTPA-enhanced HBP MR images showed a strong correlation with liver fibrosis stage and higher diagnostic performance than APRI and FIB-4 across most fibrosis thresholds. Because CV can be calculated from routine clinical MRI acquisitions without additional hardware or blood tests, it may serve as a practical biomarker for non-invasive liver fibrosis assessment. However, because this study was conducted in a Korean cohort with predominantly hepatitis B virus-related liver disease, further validation in other ethnic groups and etiologies is needed. FAU - Kim, Youe Ree AU - Kim YR AUID- ORCID: 0000-0001-5615-9721 AD - Department of Radiology, Wonkwang University Hospital, 895 Muwang-ro, Iksan 54538, Jeollabuk-do, Republic of Korea. FAU - Kim, Tae-Hoon AU - Kim TH AUID- ORCID: 0000-0002-2552-0665 AD - Medical Convergence Research Center, Wonkwang University, 460 Iksandae-ro, Iksan 54538, Jeollabuk-do, Republic of Korea. FAU - Jeong, Chang-Won AU - Jeong CW AUID- ORCID: 0000-0002-9305-4686 AD - Medical Convergence Research Center, Wonkwang University, 460 Iksandae-ro, Iksan 54538, Jeollabuk-do, Republic of Korea. FAU - Lee, Young Hwan AU - Lee YH AD - Department of Radiology, Wonkwang University Hospital, 895 Muwang-ro, Iksan 54538, Jeollabuk-do, Republic of Korea. FAU - Cho, Eun Young AU - Cho EY AD - Department of Internal Medicine, Wonkwang University Hospital, 895 Muwang-ro, Iksan 54538, Jeollabuk-do, Republic of Korea. FAU - Choi, Keum Ha AU - Choi KH AD - Department of Pathology, Wonkwang University Hospital, 895 Muwang-ro, Iksan 54538, Jeollabuk-do, Republic of Korea. FAU - Jeong, Yong Yeon AU - Jeong YY AUID- ORCID: 0000-0001-6096-3130 AD - Department of Radiology, Chonnam National University Hwasun Hospital, 322 Seoyang-ro, Hwasun 58128, Jeollanam-do, Republic of Korea. FAU - Heo, Suk Hee AU - Heo SH AD - Department of Radiology, Chonnam National University Hwasun Hospital, 322 Seoyang-ro, Hwasun 58128, Jeollanam-do, Republic of Korea. FAU - Kim, Kyung Won AU - Kim KW AUID- ORCID: 0000-0002-1532-5970 AD - Department of Radiology, Asan Medical Center, 88 Olympic-ro 43-gil, Seoul 05505, Republic of Korea. FAU - Ko, Yousun AU - Ko Y AUID- ORCID: 0000-0002-2181-9555 AD - Biomedical Research Center, Asan Institute for Life Sciences, Asan Medical Center, 88 Olympic-ro 43-gil, Seoul 05505, Republic of Korea. FAU - Shin, Youngbin AU - Shin Y AD - Biomedical Research Center, Asan Institute for Life Sciences, Asan Medical Center, 88 Olympic-ro 43-gil, Seoul 05505, Republic of Korea. FAU - Kang, Dong Min AU - Kang DM AUID- ORCID: 0000-0003-4758-3365 AD - Department of Radiology, Wonkwang University Hospital, 895 Muwang-ro, Iksan 54538, Jeollabuk-do, Republic of Korea. FAU - Yoon, Kwon-Ha AU - Yoon KH AD - Department of Radiology, Wonkwang University Hospital, 895 Muwang-ro, Iksan 54538, Jeollabuk-do, Republic of Korea. LA - eng GR - Bayer Korea/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706113 OTO - NOTNLM OT - METAVIR OT - coefficient of variation OT - gadoxetic acid OT - hepatobiliary phase OT - liver fibrosis OT - non-invasive biomarker OT - quantitative MRI COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analysis, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:39 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - jcm15207996 [pii] AID - jcm-15-07996 [pii] AID - 10.3390/jcm15207996 [doi] PST - epublish SO - J Clin Med. 2026 Oct 15;15(20):7996. doi: 10.3390/jcm15207996. PMID- 42941509 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Oral Lichen Planus: Current Evidence, Clinical Challenges, and the Search for Optimal Management. LID - 10.3390/jcm15207995 [doi] LID - 7995 AB - Oral lichen planus (OLP) is a chronic, immune-mediated inflammatory disease of the oral mucosa with an incompletely understood etiology and a diverse clinical presentation. Because OLP is often refractory and follows a chronic or relapsing course, accurate diagnosis and appropriate long-term management remain important clinical challenges. The diagnosis of OLP is based on clinical findings and histopathological examination, with consideration of a variety of conditions that may mimic OLP, including oral lichenoid lesions, autoimmune blistering diseases, candidiasis, and leukoplakia. Management is primarily aimed at controlling inflammation and alleviating symptoms. Elimination of precipitating or aggravating factors, improvement of oral hygiene, and appropriate dental treatment are important components of long-term disease management. Topical corticosteroids remain the mainstay of local therapy, while topical calcineurin inhibitors, such as tacrolimus and pimecrolimus, may provide useful alternatives, particularly in steroid-refractory cases. Laser therapy may also be considered as an adjunctive treatment in selected patients. Systemic corticosteroids are generally reserved for severe, extensive, or refractory disease. In addition, various adjunctive and alternative therapies, including herbal medicines, phytochemicals, vitamins, micronutrients, hydroxychloroquine, and methotrexate, have been investigated. Although several of these approaches have shown promising therapeutic effects, the available evidence remains limited and further high-quality clinical studies are required. OLP is currently classified among the oral potentially malignant disorders, although the magnitude of its malignant transformation risk remains debated. Therefore, long-term surveillance is important because some patients may develop oral squamous cell carcinoma. At present, the management of OLP focuses primarily on symptom relief and disease control rather than cure. A comprehensive and individualized approach that integrates accurate diagnosis, elimination of aggravating factors, oral health management, appropriate selection of topical and systemic therapies, and long-term surveillance is therefore important. Further clarification of the underlying mechanisms of OLP and well-designed clinical studies may contribute to more effective and individualized approaches to its diagnosis and management. FAU - Abe, Masanobu AU - Abe M AUID- ORCID: 0000-0001-9472-5077 AD - Department of Dentistry & Oral Surgery, The Cancer Institute Hospital of Japanese Foundation for Cancer Research, 3-8-31 Ariake, Koto, Tokyo 135-8550, Japan. LA - eng PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706547 OTO - NOTNLM OT - diagnosis OT - malignant transformation OT - management OT - oral lichen planus OT - oral potentially malignant disorders COIS- The author declares no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:39 PHST- 2026/09/13 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - jcm15207995 [pii] AID - jcm-15-07995 [pii] AID - 10.3390/jcm15207995 [doi] PST - epublish SO - J Clin Med. 2026 Oct 15;15(20):7995. doi: 10.3390/jcm15207995. PMID- 42941504 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Percutaneous Electrolysis and Microelectrolysis for Rotator Cuff Tendinopathy: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. LID - 10.3390/jcm15207983 [doi] LID - 7983 AB - Background/Objectives: Rotator cuff tendinopathy frequently contributes to shoulder pain and functional limitation. This systematic review and meta-analysis examined the effects of percutaneous electrolysis (EL) and microelectrolysis (MEL) on pain, shoulder-related disability, range of motion (ROM), and pressure pain threshold (PPT), and summarized treatment-related adverse events. Methods: Randomized controlled trials (RCTs) were identified through searches of Scopus, PubMed, CINAHL, Web of Science, the Cochrane Library, MEDLINE, Google Scholar, ScienceDirect, and the Physiotherapy Evidence Database (PEDro), from database inception to 14 September 2026. Methodological quality, risk of bias, and certainty of evidence were evaluated using the PEDro scale, Cochrane Risk of Bias 2 tool, and GRADE framework, respectively. Random-effects models were used to pool standardized mean differences (SMDs) or mean differences (MDs) with 95% confidence intervals (CIs). Results: Eight RCTs involving 330 participants were included. The overall post-treatment effect favored electrolysis-based interventions for pain intensity, with a large effect size (SMD = -1.44; 95% CI: -2.21 to -0.67). Pain improvements persisted at the first follow-up within the first 3 months (MD = -2.25; 95% CI: -3.40 to -1.09) and at 6 months (MD = -2.89; 95% CI: -3.68 to -2.10). Shoulder-related disability also favored electrolysis-based interventions (SMD = -0.81; 95% CI: -1.25 to -0.36). Improvements were also observed for selected ROM outcomes, whereas evidence for PPT remained inconclusive. Adverse-event reporting varied across trials; reported events were predominantly transient and non-serious, and no serious treatment-related adverse events were reported. Overall certainty of evidence ranged from low to moderate. Conclusions: Electrolysis-based interventions may improve pain intensity and shoulder-related disability and may provide benefits for selected ROM outcomes in adults with rotator cuff tendinopathy. However, confidence in the pain findings remains limited, with low-certainty evidence for the primary post-treatment analysis and low-to-moderate certainty at follow-up. No serious treatment-related adverse events were reported, although the available RCT evidence was insufficient to estimate adverse-event incidence precisely. Adequately powered RCTs directly comparing EL and MEL are needed to clarify their relative clinical effectiveness. FAU - de la Barra Ortiz, Hernan Andres AU - de la Barra Ortiz HA AUID- ORCID: 0000-0002-3927-1743 AD - Exercise and Rehabilitation Sciences Institute, School of Physical Therapy, Faculty of Rehabilitation Sciences, Universidad Andres Bello, Santiago 7591538, Chile. FAU - Lange, Claudio Chamorro AU - Lange CC AUID- ORCID: 0000-0002-6703-8842 AD - Exercise and Rehabilitation Sciences Institute, School of Physical Therapy, Faculty of Rehabilitation Sciences, Universidad Andres Bello, Santiago 7591538, Chile. FAU - Ronzio, Oscar AU - Ronzio O AUID- ORCID: 0000-0002-0004-5992 AD - Department of Physical Therapy-Kinesiology, Universidad Maimonides, Buenos Aires C1405BCK, Argentina. LA - eng PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706561 OTO - NOTNLM OT - microelectrolysis OT - musculoskeletal disorders OT - percutaneous electrolysis OT - rotator cuff tendinopathy OT - shoulder pain OT - systematic review COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:39 PHST- 2026/07/22 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - jcm15207983 [pii] AID - jcm-15-07983 [pii] AID - 10.3390/jcm15207983 [doi] PST - epublish SO - J Clin Med. 2026 Oct 15;15(20):7983. doi: 10.3390/jcm15207983. PMID- 42941497 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Diagnostic Value of Gastrointestinal Ultrasound (GIUS), Including Doppler and Contrast-Enhanced Ultrasound (CEUS), in the Assessment of Inflammatory Bowel Disease Activity: A Scoping Review. LID - 10.3390/jcm15207984 [doi] LID - 7984 AB - Background/Objectives: Treat-to-target management of inflammatory bowel disease (IBD) requires frequent, accurate assessment of disease activity. Endoscopy remains the reference standard, but its invasiveness limits repeated use, which has increased interest in gastrointestinal ultrasound (GIUS). To map the evidence on the diagnostic value of GIUS, including Doppler and contrast-enhanced ultrasound (CEUS), for IBD activity, with reference to endoscopy and serological/immunological biomarkers. Methods: This scoping review followed PRISMA-ScR. PubMed, Scopus, Web of Science and Embase were searched from January 2018 to March 2026, supplemented by reference-list screening. Two reviewers screened records independently. No formal risk-of-bias assessment was performed, and the synthesis was narrative. Results: Of 1284 records, 15 primary studies were included. Published meta-analyses report sensitivity of 85-91% and specificity of 83-96% for GIUS versus endoscopy; these are ranges from published sources, not estimates pooled by us. Bowel wall thickness (BWT) > 3 mm was the most frequently evaluated parameter. Color Doppler (Limberg grading) and CEUS added vascularity and perfusion information, and CEUS showed the highest reported accuracy in the meta-analytic literature, although data are limited. Combined GIUS-biomarker approaches reported accuracies of about 88-92% in single-center studies. Cytokine data (including IL-8) came mainly from non-GIUS studies and are hypothesis-generating. Conclusions: GIUS, alone or with biomarkers, is a promising complementary tool for monitoring IBD, mainly in moderate-to-severe disease. Because the evidence is heterogeneous, of unassessed quality and lacks randomized comparisons, it does not currently support replacing endoscopy or deferring it safely. Standardized protocols and randomized trials are needed. FAU - Korczyk, Radoslaw AU - Korczyk R AD - Department of Internal Medicine, Angiology and Physical Medicine, Medical University of Silesia in Katowice, 40-055 Katowice, Poland. FAU - Zlotnik, Angelika AU - Zlotnik A AD - Department of Internal Medicine, Angiology and Physical Medicine, Medical University of Silesia in Katowice, 40-055 Katowice, Poland. FAU - Scierski, Pawel AU - Scierski P AD - Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 40-751 Katowice, Poland. FAU - Lipka, Barbara AU - Lipka B AD - Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 40-751 Katowice, Poland. FAU - Oles, Piotr AU - Oles P AD - Department of Internal Medicine, Angiology and Physical Medicine, Medical University of Silesia in Katowice, 40-055 Katowice, Poland. FAU - Kawczyk-Krupka, Aleksandra AU - Kawczyk-Krupka A AUID- ORCID: 0000-0003-1284-3809 AD - Department of Internal Medicine, Angiology and Physical Medicine, Medical University of Silesia in Katowice, 40-055 Katowice, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705956 OTO - NOTNLM OT - Crohn's disease OT - Doppler ultrasound OT - bowel wall thickness OT - contrast-enhanced ultrasound OT - gastrointestinal ultrasound OT - inflammatory bowel disease OT - ulcerative colitis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:39 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - jcm15207984 [pii] AID - jcm-15-07984 [pii] AID - 10.3390/jcm15207984 [doi] PST - epublish SO - J Clin Med. 2026 Oct 15;15(20):7984. doi: 10.3390/jcm15207984. PMID- 42941488 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Mechanical Complications After Acute Myocardial Infarction: Contemporary Management, Treatment Selection, and Outcomes in a 10-Year Regional Referral Network. LID - 10.3390/jcm15207975 [doi] LID - 7975 AB - Background: Mechanical complications (MCs) after acute myocardial infarction (AMI) are now uncommon but remain highly lethal, and contemporary data encompassing the full spectrum of rupture phenotypes are limited. Methods: We retrospectively identified all consecutive adults with post-AMI MCs managed at a tertiary regional referral center from 2015 to 2025. MCs included ventricular septal rupture (VSR), left ventricular free-wall rupture (LVFWR), contained rupture/pseudoaneurysm, and papillary muscle rupture (PMR). The primary endpoint was 30-day all-cause mortality. Exploratory analyses assessed treatment patterns by age, sex, geographical distance, and area-level income. Results: Among 59 patients (mean age 71.2 +/- 10.7 years; 50.8% women), 17 (28.8%) had isolated VSR, 21 (35.6%) LVFWR, 10 (16.9%) combined VSR and contained LVFWR/pseudoaneurysm, and 11 (18.6%) PMR. Temporary mechanical circulatory support (MCS) and surgery were each used in 35 patients (59.3%). Thirty-day mortality was 59.3%. Among patients undergoing definitive invasive treatment, mortality was 45.7%. Older age was associated with higher 30-day mortality (OR per year 1.09, 95% CI 1.03-1.16), whereas surgery was associated with lower observed mortality (OR 0.22, 95% CI 0.07-0.73), acknowledging major treatment selection and survivor biases. Patients aged >/=75 years received MCS less frequently than younger patients (29.2% vs. 80.0%), and women underwent surgery less frequently than men (46.7% vs. 72.4%). Distance and area-level income were not associated with 30-day mortality. Conclusions: In a contemporary regional referral network, post-AMI MCs remained associated with extreme early mortality. Age and treatment selection were major determinants of observed outcomes, while marked age- and sex-related differences in treatment intensity warrant confirmation in larger multicenter cohorts. FAU - Alen, Alberto AU - Alen A AUID- ORCID: 0000-0002-0771-4650 AD - Cardiology, Hospital Universitario Central de Asturias (HUCA), 33011 Oviedo, Spain. AD - Instituto de Investigacion del Principado de Asturias (ISPA), 33011 Oviedo, Spain. FAU - Alvarez-Gato, Andres AU - Alvarez-Gato A AUID- ORCID: 0009-0007-9255-4092 AD - Cardiology, Hospital Universitario Central de Asturias (HUCA), 33011 Oviedo, Spain. FAU - Sanchez, Manuel AU - Sanchez M AD - Cardiology, Hospital Universitario Central de Asturias (HUCA), 33011 Oviedo, Spain. FAU - Gonzalez-Urbistondo, Francisco AU - Gonzalez-Urbistondo F AD - Cardiology, Hospital de Sierrallana, 39300 Torrelavega, Spain. FAU - Salgado, Maria AU - Salgado M AUID- ORCID: 0000-0001-6697-6274 AD - Cardiology, Hospital Universitario Marques de Valdecilla, 39008 Santander, Spain. FAU - Alvarez-Velasco, Rut AU - Alvarez-Velasco R AUID- ORCID: 0000-0002-2837-7374 AD - Cardiology, Hospital Universitario Central de Asturias (HUCA), 33011 Oviedo, Spain. AD - Instituto de Investigacion del Principado de Asturias (ISPA), 33011 Oviedo, Spain. FAU - Rodriguez-Yago, Miguel Angel AU - Rodriguez-Yago MA AUID- ORCID: 0000-0001-6811-2322 AD - Cardiac Surgery Critic Care Unit, Hospital Universitario Central de Asturias (HUCA), 33006 Oviedo, Spain. FAU - Soto, Maria Isabel AU - Soto MI AD - Cardiology, Hospital Universitario Central de Asturias (HUCA), 33011 Oviedo, Spain. FAU - Silva, Jacobo AU - Silva J AD - Instituto de Investigacion del Principado de Asturias (ISPA), 33011 Oviedo, Spain. AD - Cardiac Surgery, Hospital Universitario Central de Asturias (HUCA), 33006 Oviedo, Spain. AD - Faculty of Medicine, University of Oviedo, 33006 Oviedo, Spain. FAU - Avanzas, Pablo AU - Avanzas P AUID- ORCID: 0000-0002-4958-6108 AD - Cardiology, Hospital Universitario Central de Asturias (HUCA), 33011 Oviedo, Spain. AD - Instituto de Investigacion del Principado de Asturias (ISPA), 33011 Oviedo, Spain. AD - Faculty of Medicine, University of Oviedo, 33006 Oviedo, Spain. FAU - Lorca, Rebeca AU - Lorca R AUID- ORCID: 0000-0002-7803-4011 AD - Cardiology, Hospital Universitario Central de Asturias (HUCA), 33011 Oviedo, Spain. AD - Instituto de Investigacion del Principado de Asturias (ISPA), 33011 Oviedo, Spain. AD - Faculty of Medicine, University of Oviedo, 33006 Oviedo, Spain. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706350 OTO - NOTNLM OT - acute myocardial infarction OT - cardiac surgery OT - free-wall rupture OT - mechanical circulatory support OT - mechanical complications OT - papillary muscle rupture OT - ventricular septal rupture COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:39 PHST- 2026/09/22 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - jcm15207975 [pii] AID - jcm-15-07975 [pii] AID - 10.3390/jcm15207975 [doi] PST - epublish SO - J Clin Med. 2026 Oct 15;15(20):7975. doi: 10.3390/jcm15207975. PMID- 42941485 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Photobiomodulation Therapy in Head and Neck Cancer Patients Undergoing Chemoradiotherapy: Role in Toxicities Prevention. LID - 10.3390/jcm15207968 [doi] LID - 7968 AB - Background: Chemoradiotherapy (CRT) for head and neck cancer (HNC) is frequently associated with severe acute toxicities that compromise treatment adherence and quality of life. This study evaluated the effectiveness of photobiomodulation (PBM) in reducing CRT-induced toxicities in a real-world cohort. Methods: Between May 2021 and July 2024, we retrospectively analyzed 117 HNC patients treated with definitive or adjuvant CRT and concomitant PBM: a non-invasive application of red or near-infrared light to cutaneous and mucosal surfaces to modulate inflammatory responses, using 620/820 nm or 650 nm devices, with irradiation times of 2-12 min and energy densities of 3-12 J/cm(2) according to the treatment protocol. Toxicities were graded according to CTCAE v 5.0 at baseline, during treatment, at treatment completion, and throughout follow-up. Associations with toxicity were assessed with logistic regression for repeated measurements (generalized estimating equations). Patients were stratified according to PBM exposure (<10 vs. >/=10 sessions). Results: Median follow-up was 16 months. At treatment completion, grade 2-3 oral mucositis and radiodermatitis occurred in 38.5% and 25.6% of patients, respectively, both lower than rates reported in historical cohorts without PBM. Patients receiving >/=10 PBM sessions (72 pts) experienced significantly lower rates of grade 2-3 odynophagia at treatment completion (16.7% vs. 42.2%, p = 0.005) and persistent dysphagia at the last follow-up (12.5% vs. 33.3%, p = 0.009). PBM exposure was not associated with significant differences in weight loss, progression-free survival, or overall survival. Conclusions: This study provides real-world data on toxicity outcomes among HNC patients receiving PBM, with lower rates of selected toxicities observed among patients receiving >/=10 PBM sessions. Although the retrospective design and lack of a control group limit causal inference, these findings provide a strong rationale for prospective randomized trials to define the optimal PBM schedule and validate its clinical impact. FAU - Rotondi, Margherita AU - Rotondi M AD - Department of Radiation Oncology, Institute Gustave Roussy, 94805 Villejuif, France. FAU - Sun, Roger AU - Sun R AUID- ORCID: 0000-0001-9866-6449 AD - Department of Radiation Oncology, Institute Gustave Roussy, 94805 Villejuif, France. AD - INSERM, Radiotherapie Moleculaire et Innovation Therapeutique, 94805 Villejuif, France. FAU - El-Masri, Houssam AU - El-Masri H AUID- ORCID: 0009-0007-5632-6471 AD - Department of Radiation Oncology, Institute Gustave Roussy, 94805 Villejuif, France. FAU - Facondo, Giuseppe AU - Facondo G AUID- ORCID: 0000-0001-6056-4295 AD - Department of Radiation Oncology, Hospital Santa Maria della Misericordia, 33100 Udine, Italy. FAU - Cabulca, Andreea AU - Cabulca A AUID- ORCID: 0009-0007-7874-3380 AD - Department of Radiation Oncology, Institute Gustave Roussy, 94805 Villejuif, France. FAU - Belattar, Nahla AU - Belattar N AD - Department of Radiation Oncology, Institute Gustave Roussy, 94805 Villejuif, France. FAU - Dumitrescu, Gabriela AU - Dumitrescu G AUID- ORCID: 0009-0001-1895-5169 AD - Department of Radiation Oncology, Institute Gustave Roussy, 94805 Villejuif, France. FAU - Deutsch, Eric AU - Deutsch E AD - Department of Radiation Oncology, Institute Gustave Roussy, 94805 Villejuif, France. AD - INSERM, Radiotherapie Moleculaire et Innovation Therapeutique, 94805 Villejuif, France. FAU - Billard-Sandu, Camelia AU - Billard-Sandu C AUID- ORCID: 0000-0002-0551-8170 AD - Department of Radiation Oncology, Institute Gustave Roussy, 94805 Villejuif, France. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705853 OTO - NOTNLM OT - chemotherapy OT - head and neck cancer OT - low level laser therapy OT - orofacial complications OT - photobiomodulation OT - radiotherapy OT - supportive care OT - toxicities COIS- The authors declare that none of them have any conflicts of interest in relation to the present publication. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:39 PHST- 2026/08/06 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - jcm15207968 [pii] AID - jcm-15-07968 [pii] AID - 10.3390/jcm15207968 [doi] PST - epublish SO - J Clin Med. 2026 Oct 15;15(20):7968. doi: 10.3390/jcm15207968. PMID- 42941478 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Olopatadine in Seasonal Allergic Conjunctivitis: Efficacy, Ocular Surface Safety, and Practical Considerations for Preservative-Free Multidose Therapy. LID - 10.3390/jcm15207963 [doi] LID - 7963 AB - Background: Seasonal allergic conjunctivitis (SAC) is the most prevalent form of ocular allergy, characterized by recurrent episodes of IgE-mediated conjunctival inflammation triggered by seasonal aeroallergens (pollens). Its clinical course typically spans 4-12 weeks for individual pollen taxa and may extend considerably longer in polyallergic patients. Olopatadine hydrochloride ophthalmic solution, a selective H1-receptor antagonist with mast cell-stabilizing properties, is one of the most extensively studied topical antihistamines for SAC and is highly ranked in comparative evidence networks. Two clinically relevant aspects have received insufficient attention: the impact of benzalkonium chloride (BAK), a preservative, on the conjunctival surface, and the implications of bottle size for treatment adherence during SAC's prolonged symptomatic periods. Methods: A non-systematic narrative review of the literature was conducted. PubMed was searched through 6 October 2026 using combinations of terms addressing seasonal and allergic conjunctivitis, olopatadine, benzalkonium chloride, preservative-free formulations, ocular surface, pollen season, polyallergy/polysensitization, adherence/compliance, multidose systems, contamination, and in-use shelf life. Randomized controlled trials, comparative clinical studies, systematic reviews, meta-analyses, and clinically relevant mechanistic, toxicological, and microbiological studies were prioritized; reference lists of key articles were also screened. No formal systematic-review or PRISMA selection process was applied. Because direct comparative evidence for preservative-free versus BAK-preserved olopatadine and for bottle-size effects on adherence is lacking, evidence from related ophthalmic formulations was considered indirect and interpreted accordingly. Results: In the most recent network meta-analysis, olopatadine 0.1% was highly ranked among topical antihistamines for SAC (SUCRA 0.88) and perennial allergic conjunctivitis (PAC; SUCRA 0.85). However, this ranking should be interpreted together with pairwise comparisons and study-design heterogeneity, including CAC-based evidence in which alcaftadine has shown greater reduction of ocular itch at early post-challenge time points. Olopatadine acts primarily at the conjunctival surface, with low systemic exposure after topical administration. Current evidence does not establish that BAK-mediated enhancement of intraocular drug delivery is required; however, in conventional multidose bottles, it serves an important antimicrobial-preservative function, and any preservative-free multidose presentation must rely on validated container technology and an adequate in-use shelf life. For olopatadine 0.1% BID, a larger bottle may reduce the number of dispensing events during prolonged symptomatic periods, but a direct adherence benefit has not been demonstrated. Conclusions: Olopatadine remains an established topical treatment for SAC. Preservative-free formulations may avoid preservative-related ocular-surface exposure, but direct head-to-head evidence demonstrating superior ocular-surface outcomes for preservative-free versus BAK-preserved olopatadine is currently unavailable. Likewise, a larger bottle may be practically useful for BID regimens when its validated in-use period permits prolonged use, although bottle size alone should not be considered a proven determinant of adherence. Formulation choice should therefore integrate efficacy, dosing frequency, ocular-surface tolerability, container technology, in-use shelf life, cost, and the expected duration of allergen exposure. FAU - Ciprandi, Giorgio AU - Ciprandi G AUID- ORCID: 0000-0001-7016-8421 AD - Department of Medicine and Health Science, University of Molise, 86100 Campobasso, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706067 OTO - NOTNLM OT - antihistamine OT - benzalkonium chloride OT - mast cell stabilizer OT - ocular surface OT - olopatadine OT - pollen season OT - polysensitization OT - preservative-free OT - seasonal allergic conjunctivitis OT - treatment adherence COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:39 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - jcm15207963 [pii] AID - jcm-15-07963 [pii] AID - 10.3390/jcm15207963 [doi] PST - epublish SO - J Clin Med. 2026 Oct 15;15(20):7963. doi: 10.3390/jcm15207963. PMID- 42941476 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Gender Differences in the Clinical and Metabolic Manifestations of Anemia in Chronic Heart Failure. LID - 10.3390/jcm15207961 [doi] LID - 7961 AB - Background/Objectives: Anemia and abnormalities in iron-related biomarkers are common in chronic heart failure (CHF), but their relationships with biological sex and left ventricular ejection fraction (LVEF) remain incompletely understood. This study assessed sex-related differences in hematological, iron-related, oxidative stress, and purine metabolism biomarkers across CHF phenotypes. Methods: This cross-sectional observational study included 180 patients with CHF, classified according to sex, anemia status based on World Health Organization sex-specific hemoglobin thresholds, and LVEF category: heart failure with preserved ejection fraction (HFpEF), mildly reduced ejection fraction (HFmrEF), or reduced ejection fraction (HFrEF). Hematological parameters, serum iron, ferritin, plasma and erythrocyte purine metabolites, oxidative modification of proteins (OMB), and advanced oxidation protein products (AOPPs) were evaluated. Group comparisons were performed using the Kruskal-Wallis and Mann-Whitney U tests, with Benjamini-Hochberg false discovery rate correction for multiple testing. Results: After multiple-testing correction, none of the biomarker comparisons across LVEF categories remained statistically significant when women and men were analyzed separately. Among women with HFpEF, median ferritin was 33.0 mug/L overall and 20.35 mug/L among those with anemia; however, these values could not be used to determine the mechanism of iron deficiency. Among men, anemia was associated with a higher platelet count in HFpEF (287 versus 203 x 10(9)/L; q < 0.009) and lower serum iron concentrations in HFpEF (8.8 versus 18.1 mumol/L; q = 0.029) and HFrEF (5.5 versus 12.0 mumol/L; q = 0.036). In direct comparisons between sexes, only the higher hemoglobin concentration in men with HFmrEF remained statistically significant after correction (148 versus 121 g/L; q < 0.036). Nominal differences in plasma purines, hypoxanthine, and xanthine between anemic men and women with HFrEF did not remain statistically significant after correction (all q = 0.147). Conclusions: The findings demonstrate exploratory associations between sex, anemia status, LVEF category, and selected laboratory biomarkers but do not establish sex-specific mechanisms of anemia or purine metabolism alterations in CHF. Because transferrin saturation, transferrin-related indices, soluble transferrin receptor, hepcidin, and inflammatory biomarkers were not measured, the observed iron-related patterns cannot be classified as absolute iron deficiency or inflammation-associated functional iron restriction. Larger studies incorporating formal interaction testing, multivariable adjustment, and comprehensive assessment of iron status are required. FAU - Konysbek, Yessen AU - Konysbek Y AUID- ORCID: 0009-0000-4509-626X AD - Department of Internal Diseases, Karaganda Medical University, Karaganda 100028, Kazakhstan. FAU - Turar, Ayazhan AU - Turar A AD - <> LLP, Shymkent 160021, Kazakhstan. FAU - Molotov-Luchanskiy, Vilen B AU - Molotov-Luchanskiy VB AD - Department of Internal Diseases, Karaganda Medical University, Karaganda 100028, Kazakhstan. FAU - Ponamareva, Olga A AU - Ponamareva OA AUID- ORCID: 0000-0002-0222-5794 AD - Department of Internal Diseases, Karaganda Medical University, Karaganda 100028, Kazakhstan. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705860 OTO - NOTNLM OT - anemia OT - chronic heart failure OT - ejection fraction OT - ferritin OT - hemoglobin OT - hypoxanthine OT - iron deficiency OT - purine metabolism OT - sex differences OT - xanthine COIS- Author Ayazhan Turar is employed by <> LLP, Shymkent 160021, Kazakhstan. The author confirms her employment relationship with the company and declares that there are no conflicts of interest related to this article. The remaining authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:39 PHST- 2026/08/01 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - jcm15207961 [pii] AID - jcm-15-07961 [pii] AID - 10.3390/jcm15207961 [doi] PST - epublish SO - J Clin Med. 2026 Oct 15;15(20):7961. doi: 10.3390/jcm15207961. PMID- 42941475 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Advances in Aerobic Exercise for Pulmonary Arterial Hypertension: Mechanisms of Action and Clinical Insights. LID - 10.3390/jcm15207960 [doi] LID - 7960 AB - Pulmonary arterial hypertension (PAH) is a progressive disease characterized by elevated pulmonary artery pressure and pulmonary vascular resistance, leading to right heart failure and death in severe cases. PAH is classified as Group 1 pulmonary hypertension (PH). Although Groups 2 and 3 PH are more common, this review focuses primarily on PAH. Currently, there are three main classes of targeted drugs in clinical practice, and their main mechanism is to dilate pulmonary artery vessels. These drugs can delay the progression of PAH and improve the quality of life. However, the long-term prognosis is still not optimistic. At present, patients with PAH still need a multi-drug combination and comprehensive treatment. Aerobic exercise, as a non-pharmacological adjunctive therapy, is believed to have a positive impact on PAH treatment when performed regularly in patients with stable conditions. With the widespread application of aerobic exercise in the treatment of cardiovascular diseases, increasing research is exploring its potential in adjunctive therapy for PAH. Studies suggest that exercise-based rehabilitation incorporating aerobic exercise, when added to optimized PAH-targeted therapy, may improve exercise capacity, quality of life, and selected cardiopulmonary or hemodynamic parameters in clinically stable patients with PAH. However, evidence for reversal of pulmonary vascular remodeling or mortality benefit remains limited. This review summarizes the clinical evidence and mechanisms of aerobic exercise specifically in PAH. FAU - Wang, Tianyu AU - Wang T AD - Department of Cardiology, The Second Xiangya Hospital of Central South University, Changsha 410011, China. FAU - Tan, Yingjie AU - Tan Y AD - Department of Cardiology, The Second Xiangya Hospital of Central South University, Changsha 410011, China. FAU - Deng, Limin AU - Deng L AD - Department of Nutrition, The Second Xiangya Hospital of Central South University, Changsha 410011, China. FAU - Li, Jiang AU - Li J AUID- ORCID: 0000-0003-4904-6635 AD - Department of Cardiology, The Second Xiangya Hospital of Central South University, Changsha 410011, China. LA - eng GR - 82470055/National Natural Science Foundation of China/ GR - 2023JJ40813/Natural Science Foundation of Hunan Province/ PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705971 OTO - NOTNLM OT - aerobic exercise OT - cardiopulmonary function OT - pulmonary arterial hypertension OT - pulmonary vascular remodeling COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:39 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - jcm15207960 [pii] AID - jcm-15-07960 [pii] AID - 10.3390/jcm15207960 [doi] PST - epublish SO - J Clin Med. 2026 Oct 15;15(20):7960. doi: 10.3390/jcm15207960. PMID- 42941473 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Adjunctive Strategies to Enhance Antimicrobial Photodynamic Therapy in Oral Infections: A Systematic Review of Randomized Controlled Trials. LID - 10.3390/jcm15207959 [doi] LID - 7959 AB - Background/Objectives: Antimicrobial photodynamic therapy (aPDT) is a widely investigated adjunct for managing oral biofilm-associated bacterial and fungal infections, but poor photosensitizer penetration, aggregation, biofilm hypoxia, and protocol variability limit its clinical efficacy. Methods: This review was registered with PROSPERO (CRD420251207522) and conducted according to PRISMA 2020 guidelines. PubMed/MEDLINE, Embase, Scopus, and the Cochrane Library were searched for English-language RCTs. Eligible studies evaluated aPDT combined with physicochemical, delivery-based, light-based, biological, or host-modulating adjuncts in patients with oral biofilm-related bacterial or fungal disease, using an appropriate comparator. Data were synthesized narratively due to substantial clinical and methodological heterogeneity, and risk of bias was assessed using RoB 2. Results: Fourteen RCTs were included, predominantly addressing periodontal and peri-implant conditions, with additional studies involving orthodontic biofilms; no eligible RCT evaluated a fungal oral infection. Surfactant-based enhancement (e.g., sodium dodecyl sulfate) produced formulation- and protocol-dependent effects, with benefits reported for methylene blue and curcumin but not riboflavin. Nanocarrier-based photosensitizer delivery, evaluated in combination with Aloe vera vehicles, improved selected clinical outcomes without consistently superior microbiological effects; however, the independent contribution of the nanocarrier could not be isolated from the potential bioactive effects of the vehicle. An oxygen-releasing adjunct showed no significant between-group clinical benefit. Repeated dual-light irradiation improved bleeding on probing and pocket-related outcomes in some but not all trials. Probiotics, vitamin D, and low-level laser therapy showed inconsistent or preliminary benefits, each supported by limited trial numbers. Risk-of-bias assessments predominantly indicated low risk or some concerns, with one study judged at high overall risk. Conclusions: Enhancement of aPDT should not be regarded as a single therapeutic concept; its clinical benefit is strategy-, formulation-, and context-dependent. Current randomized evidence does not support a universally superior adjunct, highlighting the need for standardized, adequately powered trials with mechanism-oriented comparator designs. FAU - Warakomska, Aleksandra AU - Warakomska A AD - Department of Periodontal and Oral Mucosa Diseases, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-055 Katowice, Poland. FAU - Kepa, Malgorzata AU - Kepa M AUID- ORCID: 0000-0002-4013-6688 AD - Department of Microbiology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Jagiellonska 4, 41-200 Sosnowiec, Poland. FAU - Starosta, Martin AU - Starosta M AUID- ORCID: 0000-0002-6843-349X AD - Department of Dentistry, Faculty of Medicine, University of Ostrava, Syllabova 19, Ostrava-Jih, 703 00 Ostrava, Czech Republic. FAU - Lipka, Barbara AU - Lipka B AD - Department of Periodontal and Oral Mucosa Diseases, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-055 Katowice, Poland. FAU - Hupsch-Marzec, Hanna AU - Hupsch-Marzec H AD - Department of Periodontal and Oral Mucosa Diseases, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-055 Katowice, Poland. FAU - Skaba, Dariusz AU - Skaba D AUID- ORCID: 0000-0001-8732-251X AD - Department of Periodontal and Oral Mucosa Diseases, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-055 Katowice, Poland. FAU - Wiench, Rafal AU - Wiench R AUID- ORCID: 0000-0001-5673-7413 AD - Department of Periodontal and Oral Mucosa Diseases, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-055 Katowice, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705717 OTO - NOTNLM OT - adjunctive therapy OT - antimicrobial photodynamic therapy OT - oral biofilm OT - peri-implantitis OT - periodontitis OT - photosensitizer OT - randomized controlled trial COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:39 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - jcm15207959 [pii] AID - jcm-15-07959 [pii] AID - 10.3390/jcm15207959 [doi] PST - epublish SO - J Clin Med. 2026 Oct 14;15(20):7959. doi: 10.3390/jcm15207959. PMID- 42941465 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Prognostic Value of Tumor Budding and Worst Pattern of Invasion in Clinically Node-Negative Early-Stage Lip and Oral Cavity Squamous Cell Carcinoma: A Systematic Review. LID - 10.3390/jcm15207949 [doi] LID - 7949 AB - Background: Early-stage squamous cell carcinoma of the lip and oral cavity may behave aggressively despite clinically negative cervical lymph nodes. Tumor budding (TB) and worst pattern of invasion (WPOI) have been proposed as histopathological markers of invasive behavior, but their prognostic value in cT1-2N0M0 disease remains uncertain. Methods: This PRISMA 2020 systematic review included studies published as eligible full-text journal articles up to 25 April 2026 and was informed by REMARK and guidance for systematic reviews of prognostic factor studies. PubMed and Scopus were searched as bibliographic databases, supplemented by ScienceDirect, Semantic Scholar, and backward and forward citation searching. Eligible studies assessed TB and/or WPOI in resection specimens from surgically treated cT1-2N0M0 lip/oral cavity squamous cell carcinoma and reported nodal, recurrence, or eligible survival outcomes. Risk of bias was evaluated using the Quality In Prognosis Studies (QUIPS) tool. Results: Thirty-eight reports met eligibility criteria; linked or partially overlapping cohorts were treated as related evidence to avoid double counting. Higher-risk WPOI and high-grade TB were associated with cervical nodal disease, locoregional recurrence, and adverse survival across multiple reports, although adjusted effects varied. Eligible survival outcomes included disease-free survival, recurrence-free survival, disease-specific/cancer-specific survival, and overall survival; progression-free survival was excluded. Conclusions: TB and WPOI may provide complementary postoperative prognostic information in clinically node-negative early-stage lip and oral cavity squamous cell carcinoma. Heterogeneity in definitions, thresholds, cervical management, endpoints, and statistical adjustment precluded a single pooled estimate and limits immediate clinical implementation. Neither marker should currently be used as an isolated determinant of treatment decisions. FAU - Moldovan, Madalina Anca AU - Moldovan MA AUID- ORCID: 0000-0002-6141-515X AD - Department of Oro-Maxillo-Facial Surgery and Implantology, Faculty of Dental Medicine, Iuliu Hatieganu University, 400012 Cluj-Napoca, Romania. FAU - Filip, Laura-Veronica AU - Filip LV AD - Private Practice, Filip Laura-Veronica, 400584 Cluj-Napoca, Romania. FAU - Cumpata, Cristian Niky AU - Cumpata CN AUID- ORCID: 0000-0001-5414-0031 AD - Department of Oral and Maxillofacial Surgery, Faculty of Dental Medicine, Titu Maiorescu University, 031593 Bucharest, Romania. FAU - Bumbu, Bogdan Andrei AU - Bumbu BA AD - Department of Dental Medicine, University of Oradea, 410073 Bihor, Romania. FAU - Di Francesco, Paolo AU - Di Francesco P AUID- ORCID: 0009-0005-5304-3690 AD - Department of Prosthodontics, Faculty of Dental Medicine, Titu Maiorescu University, 031593 Bucharest, Romania. FAU - Roman, Calin Rares AU - Roman CR AUID- ORCID: 0000-0003-0620-2845 AD - Department of Oro-Maxillo-Facial Surgery and Implantology, Faculty of Dental Medicine, Iuliu Hatieganu University, 400012 Cluj-Napoca, Romania. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705793 OTO - NOTNLM OT - lip and oral cavity squamous cell carcinoma OT - locoregional recurrence OT - occult cervical metastasis OT - prognostic markers OT - systematic review OT - tumor budding OT - worst pattern of invasion COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:39 PHST- 2026/09/05 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - jcm15207949 [pii] AID - jcm-15-07949 [pii] AID - 10.3390/jcm15207949 [doi] PST - epublish SO - J Clin Med. 2026 Oct 14;15(20):7949. doi: 10.3390/jcm15207949. PMID- 42941464 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Radiographic Changes and Recorded Complications After Nonabsorbable Endosinotarsal Screw Arthroereisis for Symptomatic Pediatric Flexible Flatfoot: A Retrospective Cohort Study. LID - 10.3390/jcm15207950 [doi] LID - 7950 AB - Background: Subtalar arthroereisis is used for selected children with symptomatic flexible flatfoot that persists despite nonoperative management, but published evidence is limited by heterogeneous indications and frequent failure to account for bilateral observations. This study evaluated radiographic change and the complications recorded after nonabsorbable endosinotarsal screw arthroereisis. Methods: We retrospectively reviewed consecutive patients aged 8-15 years treated at a tertiary pediatric orthopedic center between 2018 and 2024. Eligible patients had symptomatic flexible planovalgus deformity, failure of at least 6 months of conservative treatment, isolated screw arthroereisis, and complete weight-bearing radiographs obtained preoperatively and at 12 months postoperatively. Meary's angle, the Costa-Bertani angle, Kite's angle, and calcaneal pitch were analyzed using linear mixed-effects models with repeated observations at the foot level nested within patients. Results: Seventy-four patients (141 feet) were included in the outcome analysis; 67 patients (90.5%) underwent bilateral procedures. Mean age was 11.5 +/- 1.6 years, and radiographic outcomes were assessed at 12 months in all patients; mean clinical follow-up was 18.3 +/- 3.7 months. Interobserver reliability was good to excellent. Meary's angle decreased from 19.0 degrees +/- 8.5 degrees to 7.7 degrees +/- 5.6 degrees (p < 0.001). The Costa-Bertani angle decreased by 9.0 degrees (p < 0.001), Kite's angle by 5.5 degrees (p < 0.001), and calcaneal pitch increased by 1.7 degrees (95% CI, 1.0 to 2.4; Holm-adjusted p = 0.001). Sinus tarsi pain occurred in 13 patients (17.6%) and resolved conservatively in 11. Implant removal was required in two patients (2.7%), and one patient underwent reoperation for loss of correction (1.4%). No deep infection or neurovascular injury was recorded. Conclusions: Nonabsorbable endosinotarsal screw arthroereisis was associated with radiographic improvement at the standardized 12-month assessment. Major recorded adverse events and reoperations were uncommon among the patients with available follow-up, whereas residual sinus tarsi pain was recorded in approximately one in six patients, and the implant was not removed in 97.3% of patients. The postoperative mean values indicate partial correction rather than uniform radiographic normalization. Because patient-reported and functional outcomes were unavailable, clinical benefit cannot be inferred from angular changes alone. FAU - De Salvatore, Sergio AU - De Salvatore S AUID- ORCID: 0000-0002-9111-3638 AD - Orthopaedics and Traumatology Unit, Department of Surgery, Bambino Gesu Children's Hospital, 00165 Rome, Italy. FAU - Testa, Costanzo AU - Testa C AD - Department of Orthopaedics and Traumatology, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00136 Rome, Italy. FAU - Salera, Silvia AU - Salera S AD - Department of Orthopaedics and Traumatology, Policlinico Tor Vergata, 00133 Rome, Italy. FAU - Oggiano, Leonardo AU - Oggiano L AUID- ORCID: 0000-0001-6933-0321 AD - Orthopaedics and Traumatology Unit, Department of Surgery, Bambino Gesu Children's Hospital, 00165 Rome, Italy. FAU - Costici, Edoardo AU - Costici E AD - Faculty of Medicine and Surgery, Saint Camillus International University of Health Sciences, 00131 Rome, Italy. FAU - Donati, Fabrizio AU - Donati F AD - Orthopaedics and Traumatology Unit, Department of Surgery, Bambino Gesu Children's Hospital, 00165 Rome, Italy. FAU - Maglione, Pier Luigi AU - Maglione PL AD - Orthopaedics and Traumatology Unit, Department of Surgery, Bambino Gesu Children's Hospital, 00165 Rome, Italy. FAU - Brigato, Paolo AU - Brigato P AUID- ORCID: 0000-0002-8163-3787 AD - Research Unit of Orthopaedic and Trauma Surgery, Department of Medicine and Surgery, Universita Campus Bio-Medico di Roma, Via Alvaro del Portillo 21, 00128 Rome, Italy. FAU - Russo, Rosa AU - Russo R AD - Orthopaedics and Traumatology Unit, Department of Surgery, Bambino Gesu Children's Hospital, 00165 Rome, Italy. FAU - Costici, Pier Francesco AU - Costici PF AD - Orthopaedics and Traumatology Unit, Department of Surgery, Bambino Gesu Children's Hospital, 00165 Rome, Italy. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706615 OTO - NOTNLM OT - endosinotarsal screw OT - flexible flatfoot OT - pediatric orthopedics OT - pes planovalgus OT - radiographic outcomes OT - subtalar arthroereisis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:39 PHST- 2026/07/12 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - jcm15207950 [pii] AID - jcm-15-07950 [pii] AID - 10.3390/jcm15207950 [doi] PST - epublish SO - J Clin Med. 2026 Oct 14;15(20):7950. doi: 10.3390/jcm15207950. PMID- 42941463 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Predictive Models of Morbidity and Mortality in Gynaecological Cancer Surgery: A Systematic Review. LID - 10.3390/jcm15207948 [doi] LID - 7948 AB - Background/Objectives: Gynaecological-oncological surgery involves substantial perioperative morbidity and mortality risks. Reliable preoperative risk stratification could inform consent, shared decision-making and perioperative optimisation. Several prediction models exist, but their performance and methodological quality in this population remain uncertain. We aimed to provide a comprehensive synthesis of existing evidence to guide the selection and development of prediction tools in this population. Methods: We searched MEDLINE and EMBASE using a prospective protocol (PROSPERO CRD420251179495) and included studies developing or validating multivariable prediction models or risk scores for graded postoperative morbidity/mortality after gynaecological-oncological surgery. Risk of bias and applicability were assessed using the Prediction model Risk Of Bias Assessment Tool (PROBAST) at the study (publication) level. Findings were synthesised narratively. Results: Twenty-eight studies (75,999 patient records, including overlapping cohorts) were included (23 retrospective, five prospective; sample sizes 100-21,359). Models were multifactorial (n = 17), including generic and gynaecological-oncological-specific tools, frailty-based (n = 8) models or biomarker and index-based (n = 3) models. A numerical c-statistic was reported in 20 studies (binary c-statistics 0.50-0.92 across heterogeneous outcome definitions), calibration was reported in nine studies, and internal validation was reported in thirteen. No frailty-based model reported calibration or validation. One model specific to ovarian cytoreductive surgery was independently externally validated, retaining discrimination (c-index 0.715) with reduced calibration. Generic tools performed poorly, (c-statistics ranged from 0.529-0.644). Twenty studies were at high risk of bias, predominantly within the analysis domain. Conclusions: Existing models show, at best, moderate discrimination, limited calibration and infrequent external validation within the gynaecological-oncological surgical population. No model can currently be recommended for routine clinical practice. Further independent external validation of specialty-specific models in local settings, with full calibration reporting, is needed to support preoperative counselling. FAU - Fernandes, Katie Avelina AU - Fernandes KA AD - Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London E1 2AD, UK. FAU - Vidanagamage, Sonu Krishel AU - Vidanagamage SK AD - Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London E1 2AD, UK. FAU - Gooneratne, Mevan AU - Gooneratne M AD - Department of Anaesthesia, Pain and Peri-Operative Medicine, Royal London Hospital, Barts Health NHS Trust, London E1 1FR, UK. FAU - Wilkinson, Robert AU - Wilkinson R AD - Department of Anaesthesia, Pain and Peri-Operative Medicine, Royal London Hospital, Barts Health NHS Trust, London E1 1FR, UK. FAU - Wells, Madeleine AU - Wells M AD - Department of Anaesthesia, Pain and Peri-Operative Medicine, Royal London Hospital, Barts Health NHS Trust, London E1 1FR, UK. FAU - Pang, Ching Ling AU - Pang CL AD - Department of Anaesthesia, Pain and Peri-Operative Medicine, Royal London Hospital, Barts Health NHS Trust, London E1 1FR, UK. FAU - May, Shaun AU - May S AUID- ORCID: 0000-0001-7995-0313 AD - Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London E1 2AD, UK. AD - Department of Anaesthesia, Pain and Peri-Operative Medicine, Royal London Hospital, Barts Health NHS Trust, London E1 1FR, UK. FAU - Ellis, Georgia Alexandra Elizabeth AU - Ellis GAE AD - Department of Anaesthesia, Pain and Peri-Operative Medicine, Royal London Hospital, Barts Health NHS Trust, London E1 1FR, UK. FAU - Brockbank, Elly AU - Brockbank E AD - Department of Gynaecological Oncology, Royal London Hospital, Barts Health NHS Trust, London E1 1FR, UK. FAU - Jeyarajah, Arjun AU - Jeyarajah A AD - Department of Gynaecological Oncology, Royal London Hospital, Barts Health NHS Trust, London E1 1FR, UK. FAU - Lawrence, Alexandra AU - Lawrence A AD - Department of Gynaecological Oncology, Royal London Hospital, Barts Health NHS Trust, London E1 1FR, UK. FAU - Phadnis, Saurabh AU - Phadnis S AD - Department of Gynaecological Oncology, Royal London Hospital, Barts Health NHS Trust, London E1 1FR, UK. FAU - Manchanda, Ranjit AU - Manchanda R AUID- ORCID: 0000-0003-3381-5057 AD - Department of Gynaecological Oncology, Royal London Hospital, Barts Health NHS Trust, London E1 1FR, UK. AD - Wolfson Institute of Population Health, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK. FAU - Oxley, Samuel George AU - Oxley SG AUID- ORCID: 0000-0003-1967-813X AD - Department of Gynaecological Oncology, Royal London Hospital, Barts Health NHS Trust, London E1 1FR, UK. AD - Wolfson Institute of Population Health, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK. FAU - Sideris, Michail AU - Sideris M AUID- ORCID: 0000-0002-4199-6700 AD - Department of Gynaecological Oncology, Royal London Hospital, Barts Health NHS Trust, London E1 1FR, UK. AD - Wolfson Institute of Population Health, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706611 OTO - NOTNLM OT - clinical prediction model OT - cytoreductive surgery OT - external validation OT - frailty OT - gynaecological oncology OT - mortality OT - postoperative complications OT - risk stratification COIS- Ranjit Manchanda declares research funding from the Eve Appeal, NHS Innovation Accelerator, British Gynaecological Cancer Society, GSK, and Yorkshire Cancer Research outside this work, as well as an honorarium for grant review from the Israel National Institute for Health Policy Research and an honorarium for advisory board membership from Astrazeneca/MSD/EGL/GSK. Ranjit Manchanda is the Topic Advisor for the NICE Guideline [GID-NG10225]-Ovarian cancer: identifying and managing familial and genetic risk. SO declares research funding from the RoseTrees trust. The other authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:39 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - jcm15207948 [pii] AID - jcm-15-07948 [pii] AID - 10.3390/jcm15207948 [doi] PST - epublish SO - J Clin Med. 2026 Oct 14;15(20):7948. doi: 10.3390/jcm15207948. PMID- 42941458 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Coffee Consumption, Serum Copeptin, and Selected Echocardiographic Parameters in Patients with ADPKD: A Cross-Sectional Study. LID - 10.3390/jcm15207943 [doi] LID - 7943 AB - Background/Objectives: Coffee consumption has been suggested to influence arginine vasopressin-related pathways and cardiovascular function. However, its relationships with serum copeptin and cardiac parameters in patients with autosomal dominant polycystic kidney disease (ADPKD) remain unclear. This study aimed to investigate the association between habitual coffee consumption and serum copeptin concentrations and selected echocardiographic parameters in patients with ADPKD. Methods: In this cross-sectional study, 73 patients with ADPKD were classified as coffee consumers (n = 35) or non-consumers (n = 38). Serum copeptin measurements were available for 62 patients. Coffee consumption was assessed using a standardized questionnaire and expressed as grams of dry coffee per month. Multivariable models were used to assess associations with log-transformed copeptin, mitral annular e' velocity, and E/e' ratio. Results: Serum copeptin levels were lower among coffee consumers than non-consumers (median, 263.51 vs. 332.77 ng/L; p = 0.013). After adjustment, coffee consumption was associated with lower log-transformed copeptin levels (B = -0.263; p = 0.047), although this association was not statistically significant when using heteroskedasticity-consistent (HC3) robust standard errors (p = 0.074). Coffee consumers had higher mitral annular e' velocity (8.61 vs. 5.86 cm/s; p < 0.001), which remained significant after adjustment (B = 2.531; p < 0.001), and a lower E/e' ratio (B = -4.548; p = 0.007). Conclusions: In this ADPKD cohort, coffee consumption was associated with lower serum copeptin levels, higher mitral annular e' velocity, and lower E/e' ratio. These findings raise the hypothesis that habitual coffee consumption may be associated with vasopressin-copeptin system activity and diastolic myocardial relaxation in patients with ADPKD. However, the copeptin association was not robust to HC3 analysis, and the echocardiographic findings should be considered exploratory. FAU - Esen, Bennur AU - Esen B AUID- ORCID: 0000-0002-6003-8970 AD - Department of Internal Medicine and Nephrology, Bagcilar Training and Research Hospital, University of Health Sciences, 34200 Istanbul, Turkiye. FAU - Atay, Ahmet Engin AU - Atay AE AUID- ORCID: 0000-0002-3711-5157 AD - Department of Internal Medicine, Bagcilar Training and Research Hospital, University of Health Sciences, 34200 Istanbul, Turkiye. FAU - Karagoz, Ferdi AU - Karagoz F AUID- ORCID: 0000-0001-9656-6970 AD - Department of Internal Medicine and Nephrology, Bagcilar Training and Research Hospital, University of Health Sciences, 34200 Istanbul, Turkiye. FAU - Sahin, Irfan AU - Sahin I AUID- ORCID: 0000-0001-9165-7566 AD - Department of Cardiology, Bagcilar Training and Research Hospital, University of Health Sciences, 34200 Istanbul, Turkiye. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705817 OTO - NOTNLM OT - autosomal dominant polycystic kidney disease OT - coffee OT - copeptin OT - diastolic function OT - echocardiography OT - e' velocity OT - vasopressin COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:39 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - jcm15207943 [pii] AID - jcm-15-07943 [pii] AID - 10.3390/jcm15207943 [doi] PST - epublish SO - J Clin Med. 2026 Oct 14;15(20):7943. doi: 10.3390/jcm15207943. PMID- 42941457 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Nociceptin/Orphanin FQ Peptide (NOP) Receptor Agonists as Novel Analgesics in Pain Medicine. LID - 10.3390/jcm15207942 [doi] LID - 7942 AB - Chronic pain is a major cause of disability worldwide, impacting around 20% of adults globally. Conventional mu-opioid peptide (MOP) receptor agonists provide effective analgesia; however, their prolonged use is restricted by severe dose-limiting toxicities such as opioid-induced respiratory depression, analgesic tolerance, hyperalgesia, physical dependence, and addiction. The goal of this narrative literature review was to compile the available data on NOP receptor-targeted analgesics, with a focus on dual NOP/MOP agonists and cebranopadol. The pharmacological mechanisms, preclinical and clinical evidence, safety profile, and potential therapeutic role in pain management were all highlighted. The nociceptin/orphanin FQ (N/OFQ) peptide (NOP) receptor system has been identified as a new pathway for managing pain through mechanisms that differ from those of classical opioid signaling. The NOP receptor is a Class A G-protein-coupled receptor (GPCR) that preferentially couples to inhibitory G(i/o) proteins. Upon activation, it suppresses adenylyl cyclase activity, lowers intracellular cyclic adenosine monophosphate (cAMP) levels, blocks voltage-gated calcium channels, and activates G-protein-gated inwardly rectifying potassium channels, thereby hyperpolarizing neurons and decreasing excitatory neurotransmitter release. NOP receptors are anatomically distributed throughout the peripheral and central nervous systems, including primary afferent sensory neurons, dorsal root ganglia, spinal dorsal horn, periaqueductal gray, rostral ventromedial medulla, central amygdala, ventral tegmental area, and nucleus accumbens. NOP activation via these pathways offers significant pain relief in acute nociception, as well as in chronic inflammatory and neuropathic pain conditions. Supraspinal activation of NOP within the mesocorticolimbic pathway influences the emotional aspect of pain by reducing corticotropin-releasing factor signaling and decreasing mesolimbic dopamine release. NOP agonism effectively provides analgesia while also attenuating drug reward and reinforcement. The development of pharmaceuticals utilizing NOP agonism has progressed from endogenous peptide analogues to selective non-peptide agonists, and more recently to bifunctional NOP/MOP (dual NMR) agonists, such as the dual NOP/MOP agonist cebranopadol, which has passed Phase I-III clinical testing. There are still significant questions about long-term safety, comparative efficacy, and the interpretation of unpublished or preliminary Phase III results, despite the fact that available clinical trials indicate analgesic efficacy with perhaps decreased respiratory and abuse-related effects. This drug achieves equianalgesic efficacy through potent dual NOP/MOP activation while significantly reducing the risk of respiratory depression and drug-liking. However, clinical translation of NOP receptor agonism is hindered by several challenges, including supraspinal pronociceptive paradoxes, species-specific pharmacology, dose-dependent sedation, and regulatory complexities associated with mixed-mechanism ligands. NOP-targeted therapies represent a promising approach for the development of next-generation, opioid-sparing analgesics in pain medicine. FAU - Shehata, Islam Mohammad AU - Shehata IM AUID- ORCID: 0000-0003-3663-3056 AD - Department of Anesthesia, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt. FAU - Ahmed Kamal, Manar AU - Ahmed Kamal M AUID- ORCID: 0000-0003-4339-1788 AD - Mit Ghamr Nephrology and Urology Hospital (MNUH), Mansoura 35612, Egypt. AD - Faculty of Medicine, Benha University, Benha 13511, Egypt. FAU - Makki, Taha Muhammad AU - Makki TM AUID- ORCID: 0009-0003-1890-098X AD - Faculty of Medicine, Modern University for Technology and Information, Cairo 11585, Egypt. FAU - Mohamed Salah Eldin, Basant AU - Mohamed Salah Eldin B AUID- ORCID: 0009-0005-8395-6685 AD - Faculty of Medicine, Modern University for Technology and Information, Cairo 11585, Egypt. FAU - Basta, Sandra Kareem AU - Basta SK AD - Faculty of Medicine, Modern University for Technology and Information, Cairo 11585, Egypt. FAU - Abdelmalak, Cathrin Amgad AU - Abdelmalak CA AUID- ORCID: 0009-0002-6927-0570 AD - Faculty of Medicine, Modern University for Technology and Information, Cairo 11585, Egypt. FAU - Rashed, Zeyad AU - Rashed Z AUID- ORCID: 0009-0000-9931-009X AD - Faculty of Medicine, Modern University for Technology and Information, Cairo 11585, Egypt. FAU - Hegazi, Abdul-Rahman A AU - Hegazi AA AUID- ORCID: 0009-0009-5463-7481 AD - Department of Anesthesiology, LSU Health Shreveport, Shreveport, LA 71103, USA. FAU - Anwar, Ahmed I AU - Anwar AI AUID- ORCID: 0000-0002-3140-357X AD - Department of Anesthesiology, LSU Health Shreveport, Shreveport, LA 71103, USA. FAU - Ward, Danielle D AU - Ward DD AD - Adneuris Therapeutics, Monmouth Junction, NJ 08852, USA. FAU - Kaye, Alan D AU - Kaye AD AUID- ORCID: 0000-0003-2464-0187 AD - Department of Anesthesiology, LSU Health Shreveport, Shreveport, LA 71103, USA. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705647 OTO - NOTNLM OT - cebranopadol OT - dual NOP/MOP agonists OT - emerging analgesics OT - nociceptin OT - opioid receptor OT - orphanin OT - pain OT - spinal cord OT - beta-arrestin COIS- Danielle Ward is a Regional Scientific Manager, Pain, at Tris Pharma, Inc. Alan D. Kaye is on the Speakers Bureau at Vertex Pharmaceutical. The other authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:39 PHST- 2026/09/01 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - jcm15207942 [pii] AID - jcm-15-07942 [pii] AID - 10.3390/jcm15207942 [doi] PST - epublish SO - J Clin Med. 2026 Oct 14;15(20):7942. doi: 10.3390/jcm15207942. PMID- 42941453 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Cardiac-Specific AI Reconstruction Versus Hybrid Iterative Reconstruction for Emergency Coronary CT Angiography: A Paired Single-Center Study Including Triple-Rule-Out CT. LID - 10.3390/jcm15207940 [doi] LID - 7940 AB - Background/Objectives: Optimizing coronary image quality is important in emergency computed tomography (CT), including coronary CT angiography (CCTA) and triple-rule-out CT angiography (TRO-CTA). We compared objective and subjective image quality between cardiac-specific artificial intelligence (AI) reconstruction and hybrid iterative reconstruction (HIR) using paired images from the same acquisition. Methods: This retrospective single-center study included 66 of 75 consecutively assessed emergency examinations: 28 standard CCTA and 38 TRO-CTA examinations acquired without pharmacologic heart-rate control. Both protocols used identical cardiac acquisition settings. AI and HIR images were reconstructed from the same acquisition with matched cardiac phases and motion-correction settings. Objective measurements were obtained at nine coronary locations, and two readers independently assessed overall image quality and perceived vessel-edge sharpness in unmasked paired comparisons. Results: AI reduced coronary noise from 45.8 +/- 8.5 to 24.2 +/- 7.5 Hounsfield units (HU; -47.3%), increased signal-to-noise ratio (SNR) from 9.0 +/- 2.4 to 20.6 +/- 5.6 (+129.6%), and increased contrast-to-noise ratio (CNR) from 14.8 +/- 4.2 to 45.8 +/- 16.4 (+209.7%; all Holm-adjusted p < 0.001). Readers 1 and 2 preferred AI for overall quality in 65/66 and 63/66 examinations and for sharpness in 64/66 and 63/66, respectively. Conclusions: Cardiac-specific AI reconstruction reduced image noise and increased SNR and CNR in emergency CCTA, including TRO-CTA, with readers generally favoring AI images for perceived quality and sharpness. Further studies should evaluate whether these image-quality improvements translate into better diagnostic performance. FAU - Ko, Sung Min AU - Ko SM AUID- ORCID: 0000-0002-7420-6269 AD - Department of Radiology, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, 20 Ilsan-ro, Wonju 26426, Republic of Korea. FAU - Kim, Hyunjung AU - Kim H AD - Department of Radiology, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, 20 Ilsan-ro, Wonju 26426, Republic of Korea. FAU - Cha, Sung-Jin AU - Cha SJ AD - Department of Radiology, Wonju Severance Christian Hospital, Yonsei University Wonju College of Medicine, 20 Ilsan-ro, Wonju 26426, Republic of Korea. FAU - Gao, Yongfeng AU - Gao Y AD - United Imaging Healthcare North America, Houston, TX 77054, USA. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706594 OTO - NOTNLM OT - artificial intelligence OT - coronary computed tomography angiography OT - deep-learning reconstruction OT - image quality OT - triple-rule-out computed tomography angiography COIS- Y.G. is an employee of United Imaging Healthcare and had no access to patient-level data and no role in patient selection, image interpretation, clinical data abstraction, statistical analysis, or the decision to submit. All other authors declare no competing interests. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:39 PHST- 2026/09/06 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - jcm15207940 [pii] AID - jcm-15-07940 [pii] AID - 10.3390/jcm15207940 [doi] PST - epublish SO - J Clin Med. 2026 Oct 14;15(20):7940. doi: 10.3390/jcm15207940. PMID- 42941448 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Salivary Flow, Biochemical Profile and Selected Antibacterial Properties of Saliva in Menopausal Women. LID - 10.3390/jcm15207934 [doi] LID - 7934 AB - Objectives: The aim of this study was to estimate differences in selected physicochemical and antibacterial properties of saliva between menopausal and premenopausal women. Methods: The study population consisted of 30 menopausal women and 22 premenopausal women assigned to the control group. Laboratory analyses included the assessment of unstimulated salivary flow rate, pH, concentration of total protein, lactoferrin, lysozyme, immunoglobulin A (IgA), inorganic phosphates, and ionized calcium. Only participants with clinically healthy periodontal tissues were included in the study. Results: Among menopausal women, statistically significant differences were observed in values of salivary flow rate, total protein, inorganic phosphates, and ionized calcium; however, no statistically significant differences for pH, lactoferrin, lysozyme, and IgA values were found. The unstimulated salivary flow rate parameter in the study group was significantly lower than in premenopausal women. Concentrations of total protein, inorganic phosphates, and ionized calcium in saliva of menopausal women were distinctly higher than in the control group. Conclusions: The results of the present study demonstrate a distinct alteration in salivary homeostasis characterized by significantly reduced salivary flow and increased concentrations of calcium, inorganic phosphate, and total protein between menopausal and premenopausal women. It suggests that menopause may predominantly affect the quantitative secretion and physicochemical composition of saliva rather than causing a generalized impairment of its antimicrobial and immune components. Longitudinal studies that control for age and other relevant factors, including menopausal duration, hormone therapy, and periodontal status, and that evaluate both salivary concentrations and individual analyte output rates are needed to clarify which salivary changes are independently associated with menopause and to determine their clinical relevance to oral health. FAU - Ciesielska, Aleksandra AU - Ciesielska A AUID- ORCID: 0000-0003-1987-4376 AD - Department of Periodontology, Medical University of Gdansk, 80-210 Gdansk, Poland. FAU - Kusiak, Aida AU - Kusiak A AUID- ORCID: 0000-0003-2846-1469 AD - Department of Periodontology, Medical University of Gdansk, 80-210 Gdansk, Poland. FAU - Grzybowska, Magdalena Emilia AU - Grzybowska ME AUID- ORCID: 0000-0002-5311-3450 AD - Department of Gynecology and Obstetrics, Medical University of Gdansk, 80-214 Gdansk, Poland. FAU - Kochanska, Barbara AU - Kochanska B AUID- ORCID: 0000-0001-5479-0704 AD - Department of Conservative Dentistry, Medical University of Gdansk, 80-210 Gdansk, Poland. FAU - Ochocinska, Jolanta AU - Ochocinska J AUID- ORCID: 0000-0002-4493-103X AD - Department of Conservative Dentistry, Medical University of Gdansk, 80-210 Gdansk, Poland. FAU - Cichonska, Dominika AU - Cichonska D AD - Department of Periodontology, Medical University of Gdansk, 80-210 Gdansk, Poland. FAU - Swietlik, Dariusz AU - Swietlik D AUID- ORCID: 0000-0002-9127-7368 AD - Department of Biostatistics and Neural Networks, Medical University of Gdansk, 80-210 Gdansk, Poland. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705555 OTO - NOTNLM OT - ionized calcium OT - menopausal women OT - physicochemical properties of saliva OT - saliva COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:39 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - jcm15207934 [pii] AID - jcm-15-07934 [pii] AID - 10.3390/jcm15207934 [doi] PST - epublish SO - J Clin Med. 2026 Oct 14;15(20):7934. doi: 10.3390/jcm15207934. PMID- 42941446 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Bilateral Internal Thoracic Artery Grafting in Coronary Surgery: A Three-Tier Decision Framework. LID - 10.3390/jcm15207932 [doi] LID - 7932 AB - The left internal mammary artery grafted to the left anterior descending artery (LAD) is the main determinant of survival in coronary artery bypass grafting, but the choice of the second graft remains controversial. The use of both mammary arteries offers a greater long-term survival benefit compared with a single mammary artery graft; however, this advantage is delayed and diminished when compared with a single mammary artery graft plus a radial artery graft. Furthermore, this approach is associated with a higher risk of sternal complications and, via mechanisms of competitive flow and steal phenomenon, of perioperative coronary malperfusion. This article proposes organising the clinical decision-making process according to three hierarchical levels: (1) absolute contraindications based on the patient's medical history that preclude the use of a particular graft (thoracic irradiation, subclavian artery disease, acute aortic dissection); (2) the haemodynamic adequacy of the graft for the target coronary territory; (3) the assessment of the risk of delayed sternal healing in the individual patient, where skeletonization has reduced the historical significance of diabetes. Overlaying these is a transversal variable represented by the harvesting technique (pedicled mammary artery vs. skeletonized mammary artery, together with the surgeon's learning curve), which re-interprets apparently unfavourable results and distinguishes the risk associated with the graft itself from that of the surgical procedure. The model aims to rationalise patient selection and reduce the persistent underutilisation of bilateral mammary artery grafting. It should be noted at the outset that this survival advantage rests largely on observational and propensity-matched evidence and has not been confirmed on an intention-to-treat basis by the only adequately powered randomised trial, so that the model proposed here is offered as a framework for selection rather than as a claim of established superiority. FAU - Di Giammarco, Gabriele AU - Di Giammarco G AUID- ORCID: 0000-0002-9940-9847 AD - Dipartimento di Neuroscienze, Imaging e Scienze Cliniche, Cattedra di Cardiochirurgia, Universita "G. d'Annunzio", 66100 Chieti, Italy. FAU - Massetti, Massimo AU - Massetti M AUID- ORCID: 0000-0002-7100-8478 AD - Dipartimento di Malattie Cardiovascolari, Cattedra di Cardiochirurgia, Universita Cattolica del Sacro Cuore, 00168 Roma, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706569 OTO - NOTNLM OT - aortocoronary bypass OT - arterial revascularisation OT - bilateral internal mammary artery OT - competitive flow OT - coronary-subclavian steal OT - deep sternal infection OT - skeletonization COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:39 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - jcm15207932 [pii] AID - jcm-15-07932 [pii] AID - 10.3390/jcm15207932 [doi] PST - epublish SO - J Clin Med. 2026 Oct 14;15(20):7932. doi: 10.3390/jcm15207932. PMID- 42941439 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Personalized Management Strategies in Traumatic Spinal Cord Injury: From Resuscitation to Definitive Surgical Care. LID - 10.3390/jcm15207923 [doi] LID - 7923 AB - Personalized management of traumatic spinal cord injury (TSCI) is an emerging approach in modern spine and trauma care. Unlike traditional protocol-driven approaches that apply uniform resuscitation, imaging, and surgical strategies across heterogeneous injury patterns, individualized care pathways that account for biological, physiological, and injury-specific variability may improve neurological outcomes and resource utilization. This review summarizes contemporary concepts in personalized TSCI management spanning prehospital and initial hospital care, resuscitation, imaging, and definitive surgical decision-making. The proposed framework is informed by evidence from clinical trials, observational studies, and emerging consensus statements, emphasizing stratified approaches based on neurological status, injury mechanism, physiological reserve, and imaging biomarkers. Selective spinal motion restriction, hemodynamic support within guideline ranges, and early decompression are supported by current guidelines, albeit based largely on low-quality evidence, whereas individualized hemodynamic targets, advanced neuroimaging, blood-based biomarkers, and artificial intelligence-based risk stratification remain emerging strategies that require prospective validation before routine clinical use. In conclusion, personalized management across the continuum of TSCI care provides a rational framework with potential clinical applications, although its effect on patient outcomes remains to be demonstrated. FAU - Sarasombath, Peem AU - Sarasombath P AD - Department of Orthopedics, Phramongkutklao Hospital and College of Medicine, Bangkok 10400, Thailand. FAU - Bovonratwet, Patawut AU - Bovonratwet P AD - Department of Orthopedic Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA. FAU - Chaichankul, Chaisiri AU - Chaichankul C AUID- ORCID: 0000-0001-5060-1435 AD - Department of Orthopedics, Phramongkutklao Hospital and College of Medicine, Bangkok 10400, Thailand. FAU - Chitragran, Roongrath AU - Chitragran R AD - Department of Orthopedics, Phramongkutklao Hospital and College of Medicine, Bangkok 10400, Thailand. FAU - Gajaseni, Pawin AU - Gajaseni P AD - Department of Orthopedics, Phramongkutklao Hospital and College of Medicine, Bangkok 10400, Thailand. FAU - Pundee, Chaiyapruk AU - Pundee C AD - Department of Orthopedics, Samitivej Srinakarin Hospital, Bangkok 10250, Thailand. FAU - Liawrungrueang, Wongthawat AU - Liawrungrueang W AUID- ORCID: 0000-0002-4491-6569 AD - Department of Orthopedics, Samitivej Srinakarin Hospital, Bangkok 10250, Thailand. FAU - Owodunni, Oluwafemi P AU - Owodunni OP AUID- ORCID: 0000-0003-4610-2574 AD - Department of Emergency Medicine, University of New Mexico School of Medicine, Albuquerque, NM 87106, USA. FAU - Chongsathidkiet, Pakawat AU - Chongsathidkiet P AUID- ORCID: 0000-0001-5363-3061 AD - Department of Neurosurgery, Duke University School of Medicine, Durham, NC 27710, USA. FAU - Bunmaprasert, Torphong AU - Bunmaprasert T AUID- ORCID: 0000-0001-7860-2640 AD - Department of Orthopedics, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705986 OTO - NOTNLM OT - pathophysiology OT - personalized management OT - prehospital OT - surgical approach OT - traumatic spinal cord injury COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:39 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - jcm15207923 [pii] AID - jcm-15-07923 [pii] AID - 10.3390/jcm15207923 [doi] PST - epublish SO - J Clin Med. 2026 Oct 14;15(20):7923. doi: 10.3390/jcm15207923. PMID- 42941437 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - High-Volume Online Hemodiafiltration: Does Patient Phenotype Matter? LID - 10.3390/jcm15207922 [doi] LID - 7922 AB - Online hemodiafiltration (OL-HDF) has been associated with improved clinical outcomes compared with conventional hemodialysis in randomized trials, particularly in studies achieving high convection volumes. Whether specific patient phenotypes derive greater benefit from OL-HDF remains uncertain. This narrative review examines the available evidence regarding potential clinical phenotypes associated with enhanced benefit from high-volume OL-HDF, including older adults, anuric patients, individuals with heart failure, patients with poor intradialytic hemodynamic tolerance, frail patients, those with increased inflammatory burden, and incident dialysis patients. Current randomized evidence has not detected convincing treatment-effect modification across the clinical subgroups examined; however, most subgroup analyses were not designed or powered to establish equivalence of treatment effects. Higher achieved convection volumes have been associated with improved outcomes, although these observational or post-randomization associations should not be interpreted as proof of a causal dose-response relationship. Nevertheless, several high-risk clinical scenarios may be associated with greater absolute clinical benefit because of an elevated baseline risk of adverse outcomes. Available data suggest that personalization of OL-HDF should focus less on selecting eligible phenotypes and more on optimizing treatment delivery according to patient characteristics, treatment goals, and technical feasibility. Future research should explore patient-centred outcomes and identify strategies to maximize the benefits of high-volume OL-HDF within a precision medicine framework. FAU - Ramos, Rosa AU - Ramos R AUID- ORCID: 0000-0003-1361-5672 AD - Department of Nephrology, Institut Clinic de Nefrologia i Urologia (ICNU), Hospital Clinic de Barcelona, C/Villarroel 170, 08036 Barcelona, Spain. AD - Faculty of Medicine and Health Sciences, Universitat de Barcelona, 08036 Barcelona, Spain. AD - Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), 08036 Barcelona, Spain. FAU - Maduell, Francisco AU - Maduell F AUID- ORCID: 0000-0002-1673-0353 AD - Department of Nephrology, Institut Clinic de Nefrologia i Urologia (ICNU), Hospital Clinic de Barcelona, C/Villarroel 170, 08036 Barcelona, Spain. AD - Faculty of Medicine and Health Sciences, Universitat de Barcelona, 08036 Barcelona, Spain. AD - Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), 08036 Barcelona, Spain. FAU - Broseta, Jose Jesus AU - Broseta JJ AUID- ORCID: 0000-0002-4559-9083 AD - Department of Nephrology, Institut Clinic de Nefrologia i Urologia (ICNU), Hospital Clinic de Barcelona, C/Villarroel 170, 08036 Barcelona, Spain. AD - Faculty of Medicine and Health Sciences, Universitat de Barcelona, 08036 Barcelona, Spain. AD - Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), 08036 Barcelona, Spain. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706591 OTO - NOTNLM OT - convection volume OT - hemodiafiltration OT - hemodialysis OT - mortality OT - patient phenotype OT - precision medicine COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:39 PHST- 2026/08/10 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - jcm15207922 [pii] AID - jcm-15-07922 [pii] AID - 10.3390/jcm15207922 [doi] PST - epublish SO - J Clin Med. 2026 Oct 14;15(20):7922. doi: 10.3390/jcm15207922. PMID- 42941435 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Arthroscopic Subscapularis Augmentation for the Treatment of Bankart Lesions: A Narrative Review of Indications, Techniques, and Comparative Outcomes. LID - 10.3390/jcm15207921 [doi] LID - 7921 AB - Recurrent anterior shoulder instability from a Bankart lesion is conventionally treated with arthroscopic Bankart repair, but recurrence remains substantial in patients with glenoid bone loss (GBL), capsular deficiency, hyperlaxity, or contact-sport participation. Arthroscopic subscapularis augmentation (ASA) tenodeses the upper third of the subscapularis to the anterior glenoid alongside Bankart repair, providing an additional anterior soft-tissue restraint. This narrative review, based on a literature search updated to September 2026, summarizes the rationale, indications, techniques, postoperative management, and outcomes of ASA. Selected observational cohorts, mostly from overlapping author groups, have reported recurrence of approximately 2.7-7.0% in high-risk patients with subcritical GBL; because these figures are compared mainly with historical series, the incremental benefit of ASA over appropriately selected comparator procedures remains uncertain. In one multicenter comparison, ASA had single-digit recurrence and fewer complications than open Latarjet, whereas Latarjet procedures had the lowest recurrence. ASA combined with bone grafting is an evolving option within the range of GBL studied so far (approximately 15-30%), not an established Latarjet equivalent. The evidence is predominantly retrospective Level III-IV with short- to mid-term follow-up, and prospective comparative studies with standardized outcome definitions are needed. FAU - Park, Jaewook AU - Park J AD - Department of Orthopedic Surgery, Dankook University Hospital, 201 Manghyang-ro, Dongnam-gu, Cheonan 31116, Republic of Korea. FAU - Yoo, Jaesung AU - Yoo J AUID- ORCID: 0000-0003-3644-6701 AD - Department of Orthopedic Surgery, Dankook University Hospital, 201 Manghyang-ro, Dongnam-gu, Cheonan 31116, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706244 OTO - NOTNLM OT - Bankart repair OT - Latarjet procedure OT - anterior shoulder instability OT - arthroscopic subscapularis augmentation OT - arthroscopy OT - glenoid bone loss OT - recurrence OT - subscapularis tenodesis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:39 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - jcm15207921 [pii] AID - jcm-15-07921 [pii] AID - 10.3390/jcm15207921 [doi] PST - epublish SO - J Clin Med. 2026 Oct 14;15(20):7921. doi: 10.3390/jcm15207921. PMID- 42941426 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Simulation-Based Training Tools for Cleft Lip Repair: A Systematic Review. LID - 10.3390/jcm15207911 [doi] LID - 7911 AB - Background: Simulation may supplement operative exposure in cleft lip surgery, but the strength and clinical relevance of evidence remain uncertain. This systematic review evaluated cleft lip repair simulators, focusing on characteristics, validity evidence, educational effectiveness, and implementation. Methods: PubMed, Scopus, Web of Science, and Embase were searched on 1 August 2026. Eligible studies were original English-language studies published in 2016-2026 evaluating cleft lip repair simulators for training or assessment. Non-original reports and studies without an eligible training or assessment component were excluded. Two reviewers independently selected studies and extracted data. Validity evidence was assessed using Messick's framework, educational effectiveness using an adapted McGaghie framework, and methodological quality using the Mixed Methods Appraisal Tool (MMAT) 2018. Findings were synthesized narratively. The protocol was submitted to PROSPERO on 5 August 2026, and registered on 11 August 2026 (CRD420261469105). Results: Twenty-four publications were included. Two publications (8.3%) were categorized as educational effectiveness not evaluated, 14 (58.3%) as McGaghie Level 1, and eight (33.3%) as Level 2; none reached Levels 3-5. Eight publications demonstrated objectively measured improvement in knowledge or performance following an educational intervention or repeated simulation. Response Process validity evidence was identified in 19 publications (79.2%), whereas Content-related validity evidence was identified in 13 publications (54.2%). Internal Structure and Relations to Other Variables were less frequently supported, and Consequences evidence was absent. Evidence was limited by small samples, heterogeneous designs, and frequent reliance on subjective outcomes. Conclusions: Cleft lip simulation can produce measurable educational benefits, but evidence of transfer to clinical performance or patient outcomes is lacking. Future studies should prioritize objective assessment, stronger validity and reliability evidence, accessible task-specific models, and evaluation of clinical transfer. No external funding was received. FAU - Jaworski, Aleksander AU - Jaworski A AUID- ORCID: 0000-0002-5646-9399 AD - Department of Plastic Surgery, St. John Paul II Specialist Medical Centre, 57-320 Polanica-Zdroj, Poland. AD - SudetiaMed Medical Center, 57-300 Klodzko, Poland. FAU - Ochman, Katarzyna AU - Ochman K AUID- ORCID: 0009-0008-8619-7428 AD - Students' Scientific Association of Computer Analysis and Artificial Intelligence at the Department of Radiology and Nuclear Medicine, Medical University of Silesia, 40-752 Katowice, Poland. FAU - Szemik, Kamil AU - Szemik K AUID- ORCID: 0009-0007-0178-4168 AD - Students' Scientific Association of Computer Analysis and Artificial Intelligence at the Department of Radiology and Nuclear Medicine, Medical University of Silesia, 40-752 Katowice, Poland. FAU - Kaczynska, Dominika AU - Kaczynska D AD - Students' Scientific Association of Computer Analysis and Artificial Intelligence at the Department of Radiology and Nuclear Medicine, Medical University of Silesia, 40-752 Katowice, Poland. AD - Department of Dermatology, School of Medicine in Katowice, Medical University of Silesia, 40-027 Katowice, Poland. FAU - Barnas, Oliwia AU - Barnas O AD - Students' Scientific Association of Computer Analysis and Artificial Intelligence at the Department of Radiology and Nuclear Medicine, Medical University of Silesia, 40-752 Katowice, Poland. FAU - Gawel, Aleksandra AU - Gawel A AD - Students' Scientific Association of Computer Analysis and Artificial Intelligence at the Department of Radiology and Nuclear Medicine, Medical University of Silesia, 40-752 Katowice, Poland. FAU - Nowojewska, Wiktoria AU - Nowojewska W AUID- ORCID: 0009-0002-5572-2847 AD - Students' Scientific Association of Computer Analysis and Artificial Intelligence at the Department of Radiology and Nuclear Medicine, Medical University of Silesia, 40-752 Katowice, Poland. FAU - Pankowska, Zofia AU - Pankowska Z AUID- ORCID: 0009-0001-1374-6448 AD - Students' Scientific Association of Computer Analysis and Artificial Intelligence at the Department of Radiology and Nuclear Medicine, Medical University of Silesia, 40-752 Katowice, Poland. FAU - Mlynek, Marta AU - Mlynek M AUID- ORCID: 0009-0006-7781-133X AD - Students' Scientific Association of Computer Analysis and Artificial Intelligence at the Department of Radiology and Nuclear Medicine, Medical University of Silesia, 40-752 Katowice, Poland. FAU - Kozuch, Anna AU - Kozuch A AUID- ORCID: 0009-0000-6315-6895 AD - Students' Scientific Association of Computer Analysis and Artificial Intelligence at the Department of Radiology and Nuclear Medicine, Medical University of Silesia, 40-752 Katowice, Poland. FAU - Kufel, Jakub AU - Kufel J AUID- ORCID: 0000-0001-7633-3600 AD - Department of Radiology and Nuclear Medicine, Medical University of Silesia, 40-752 Katowice, Poland. FAU - Latkowski, Ireneusz AU - Latkowski I AUID- ORCID: 0009-0003-0380-5015 AD - Department of Plastic Surgery, St. John Paul II Specialist Medical Centre, 57-320 Polanica-Zdroj, Poland. FAU - Drozdowska, Aneta AU - Drozdowska A AUID- ORCID: 0000-0003-2567-6827 AD - SudetiaMed Medical Center, 57-300 Klodzko, Poland. FAU - Drozdowski, Piotr AU - Drozdowski P AUID- ORCID: 0000-0001-9291-0121 AD - SudetiaMed Medical Center, 57-300 Klodzko, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706072 OTO - NOTNLM OT - cleft lip repair OT - simulation-based training OT - surgical education OT - surgical simulation OT - surgical training models COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:39 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - jcm15207911 [pii] AID - jcm-15-07911 [pii] AID - 10.3390/jcm15207911 [doi] PST - epublish SO - J Clin Med. 2026 Oct 13;15(20):7911. doi: 10.3390/jcm15207911. PMID- 42941424 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Therapeutic Lymphaticovenular Anastomosis for Breast Cancer-Related Lymphedema: A Critical Narrative Review of Comparative Evidence. LID - 10.3390/jcm15207909 [doi] LID - 7909 AB - This critical narrative review examines clinical outcomes of therapeutic lymphaticovenular anastomosis (LVA) for established breast cancer-related lymphedema and proposes a coordinated outcome assessment. MEDLINE/PubMed, Embase, Scopus, the Cochrane Library, and ClinicalTrials.gov were searched through 6 September 2026. Comparative studies were selected purposively, with clinical series, imaging and physiological studies, and evidence syntheses providing context. We examined outcomes, comparators, follow-up, and study limitations without quantitative pooling. A six-month interim randomized analysis found no convincing between-group differences in the total Lymphedema Functioning, Disability and Health (Lymph-ICF) score or volume measures. The physical domain and reported compression use favored LVA plus conservative treatment. These findings require consideration of outcome hierarchy and multiple comparisons. Selected observational studies reported improvements but were vulnerable to confounding. The proposed framework coordinates a minimum clinical dataset, including prescribed and actual compression use, with optional measurements of anastomosis status, transport, and tissue characteristics. It aligns technical and clinical assessments over time to explore their relationship. The selected clinical findings suggest possible benefits in symptoms and treatment burden but do not establish sustained independence from compression. The framework is a research proposal and does not provide validated rules for patient selection. FAU - Yusufov, Stiven AU - Yusufov S AUID- ORCID: 0000-0002-0095-1127 AD - Department of Oncology, Radiotherapy and Reconstructive Surgery, N.V. Sklifosovsky Institute of Clinical Medicine, Sechenov First Moscow State Medical University (Sechenov University), Moscow 119991, Russia. FAU - Iliina, Evgeniia AU - Iliina E AD - Department of Oncology, Radiotherapy and Reconstructive Surgery, N.V. Sklifosovsky Institute of Clinical Medicine, Sechenov First Moscow State Medical University (Sechenov University), Moscow 119991, Russia. FAU - Ermoshchenkova, Maria AU - Ermoshchenkova M AD - Department of Oncology, Radiotherapy and Reconstructive Surgery, N.V. Sklifosovsky Institute of Clinical Medicine, Sechenov First Moscow State Medical University (Sechenov University), Moscow 119991, Russia. FAU - Reshetov, Igor AU - Reshetov I AD - Department of Oncology, Radiotherapy and Reconstructive Surgery, N.V. Sklifosovsky Institute of Clinical Medicine, Sechenov First Moscow State Medical University (Sechenov University), Moscow 119991, Russia. FAU - Starceva, Olesya AU - Starceva O AD - Department of Oncology, Radiotherapy and Reconstructive Surgery, N.V. Sklifosovsky Institute of Clinical Medicine, Sechenov First Moscow State Medical University (Sechenov University), Moscow 119991, Russia. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705796 OTO - NOTNLM OT - BCRL OT - ICG lymphography OT - LVA OT - lymphedema COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:39 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - jcm15207909 [pii] AID - jcm-15-07909 [pii] AID - 10.3390/jcm15207909 [doi] PST - epublish SO - J Clin Med. 2026 Oct 13;15(20):7909. doi: 10.3390/jcm15207909. PMID- 42941422 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Patent Foramen Ovale Closure Alleviates Long-Term Drug-Refractory Epilepsy: A Case Report. LID - 10.3390/jcm15207906 [doi] LID - 7906 AB - Background: Drug-refractory epilepsy (DRE) poses a persistent clinical burden, and the identification of reversible extracranial contributing factors represents a promising strategy to improve seizure control. Patent foramen ovale (PFO) with right-to-left shunt (RLS) has been recognized as a potential modifiable driver of epileptic seizures, yet clinical data on patients with extremely long-standing DRE exceeding 40 years remain exceedingly scarce. Methods: We report a 46-year-old female patient with a 44-year history of DRE, who experienced daily secondarily generalized tonic-clonic seizures (GTCSs) despite sequential treatment with six mechanistically distinct antiseizure medications. Systematic workup was performed and the patient underwent percutaneous transcatheter PFO closure, with a follow-up period of 14 months. Results: Systematic workup confirmed severe PFO-related RLS (grade III by contrast transthoracic echocardiography, grade IV by contrast transcranial Doppler), while brain MRI revealed no definite structural epileptogenic lesions other than an old ischemic infarct in the right corona radiata. At the latest follow-up, the patient had achieved complete freedom from GTCS with a markedly reduced antiseizure medication burden, retaining only infrequent mild focal absence-like seizures. Follow-up video electroencephalography unmasked previously occult focal epileptiform discharges in the left temporal region. Conclusions: This case suggests that severe PFO-related RLS may serve as a reversible aggravating factor even in decades-long refractory epilepsy, and provides electroclinical evidence supporting a hypothetical two-hit model of seizure generalization. PFO screening may be considered in selected patients with cryptogenic drug-refractory epilepsy, particularly those with non-lesional MRI, non-localizing EEG, and morning-predominant seizures. FAU - Sun, Yaning AU - Sun Y AD - Department of Neurosurgery, Hebei Province of Children's Hospital, Shijiazhuang 050031, China. AD - Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050061, China. FAU - Zhao, Xianying AU - Zhao X AD - Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050061, China. FAU - Cui, Xuehua AU - Cui X AD - Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050061, China. FAU - Song, Xiaolei AU - Song X AD - Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050061, China. FAU - Wang, Zihao AU - Wang Z AUID- ORCID: 0009-0001-3121-4154 AD - Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050061, China. FAU - Liu, Wenbo AU - Liu W AD - Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050061, China. FAU - Zhang, Zhengyang AU - Zhang Z AD - Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050061, China. FAU - Li, Wenling AU - Li W AD - Department of Neurosurgery, The Second Hospital of Hebei Medical University, Shijiazhuang 050061, China. LA - eng GR - 23377709D/the Key Research and Development Program of Hebei Province/ PT - Case Reports PT - Journal Article DEP - 20261013 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705531 OTO - NOTNLM OT - drug-refractory epilepsy OT - electroencephalography OT - patent foramen ovale OT - percutaneous closure COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:39 PHST- 2026/09/07 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - jcm15207906 [pii] AID - jcm-15-07906 [pii] AID - 10.3390/jcm15207906 [doi] PST - epublish SO - J Clin Med. 2026 Oct 13;15(20):7906. doi: 10.3390/jcm15207906. PMID- 42941421 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Clinical, Radiographic, and Return-to-Sport Outcomes After MINAR Reconstruction for Acute High-Grade Acromioclavicular Joint Separation in Recreational Football Players. LID - 10.3390/jcm15207907 [doi] LID - 7907 AB - Background: High-grade acromioclavicular (AC) joint separations are clinically relevant injuries in contact sports, yet data specific to recreational football players remain limited. This study evaluated clinical recovery, maintenance of vertical coracoclavicular reduction, and return-to-sport outcomes after minimally invasive acromioclavicular reconstruction (MINAR) in recreational players with acute Rockwood type IV-V AC joint separation. Methods: This single-center retrospective analysis included 49 male recreational football players who sustained acute, isolated, unilateral Rockwood type IV or V acromioclavicular (AC) joint separation during football participation between January 2015 and December 2023. All patients underwent surgery within 7 days of injury using the same MINAR construct and implant platform, and all procedures were performed by the same experienced surgical team. Postoperative rehabilitation was supervised by the institutional physiotherapy department, generally three times weekly, with progression individualized according to clinical recovery. The primary outcome was the change in Constant score from preoperative assessment to 24 months. Secondary outcomes included Taft, American Shoulder and Elbow Surgeons (ASES), and visual analog scale (VAS) pain scores; minimal clinically important difference (MCID) achievement; coracoclavicular (CC) distance; return to unrestricted match play; return to preinjury football level; and exploratory comparisons according to vertical reduction status. Coracoclavicular distance was measured independently by three blinded observers, and the mean of the three measurements was used for the radiographic analyses. Radiographic loss of vertical reduction was defined a priori as a 24-month injured-to-contralateral CCD ratio greater than 1.50. Because only five patients met this criterion, comparisons according to vertical reduction status were considered exploratory and descriptive. Results: The mean age was 35.1 +/- 6.2 years, the mean body mass index was 27.9 +/- 5.2 kg/m(2), and the mean follow-up was 48.6 +/- 14.5 months. Forty-one patients had Rockwood type V injuries and eight had type IV injuries. All clinical scales improved over time (Friedman p < 0.001). The Constant score increased from 29.9 +/- 6.0 preoperatively to 94.5 +/- 3.8 at 24 months. Mean preoperative-to-24-month improvements were 64.6 points in Constant score, 6.11 points in Taft score, and 43.3 points in ASES score, while VAS pain decreased by 6.50 points. All 49 patients exceeded the prespecified Constant-score MCID threshold, and 47 of 49 patients (95.9%) exceeded the Taft-score MCID threshold. Return to unrestricted match play occurred in all patients at a mean of 239.9 +/- 43.5 days, and 44 patients (89.8%) returned to their preinjury match level. Five patients met the radiographic criterion for loss of vertical reduction (10.2%; 95% CI, 4.4-21.8). In exploratory unadjusted comparisons, these five patients returned to unrestricted match play later than patients who maintained vertical reduction (332.4 +/- 35.6 vs. 229.4 +/- 29.8 days; unadjusted mean difference, 103.0 days; 95% CI, 59.5-146.5), and none returned to the preinjury football level (0/5 vs. 44/44). These sparse subgroup comparisons were interpreted descriptively. Conclusions: In this cohort of male recreational football players with acute Rockwood type IV-V acromioclavicular joint separation, MINAR reconstruction was followed by substantial improvement in shoulder function, minimal residual pain, and a high rate of return to football. Maintenance of vertical reduction and return-to-sport outcomes showed a clinically notable pattern in exploratory unadjusted analyses; however, the reduction-loss subgroup comprised only five patients, and patient-level chronology did not uniformly support a temporal pathway from reduction loss to delayed return. These findings should therefore be regarded as hypothesis-generating rather than predictive or causal. FAU - Askin, Aydogan AU - Askin A AUID- ORCID: 0000-0003-2144-3647 AD - Department of Orthopaedics and Traumatology, Antalya Training and Research Hospital, University of Health Science, 07100 Antalya, Turkiye. FAU - Nabi, Vugar AU - Nabi V AUID- ORCID: 0000-0002-2766-5083 AD - Department of Orthopaedics and Traumatology, Antalya Training and Research Hospital, University of Health Science, 07100 Antalya, Turkiye. FAU - Duygun, Fatih AU - Duygun F AUID- ORCID: 0000-0001-7392-3058 AD - Department of Orthopaedics and Traumatology, Antalya Training and Research Hospital, University of Health Science, 07100 Antalya, Turkiye. FAU - Kilicaslan, Omer Faruk AU - Kilicaslan OF AUID- ORCID: 0000-0001-6716-4542 AD - Department of Orthopaedics and Traumatology, Antalya Training and Research Hospital, University of Health Science, 07100 Antalya, Turkiye. FAU - Etli, Ibrahim AU - Etli I AUID- ORCID: 0000-0002-0469-2062 AD - Department of Orthopaedics and Traumatology, Antalya Training and Research Hospital, University of Health Science, 07100 Antalya, Turkiye. FAU - Baz, Ali Bulent AU - Baz AB AUID- ORCID: 0000-0002-2661-7581 AD - Department of Orthopaedics and Traumatology, Antalya Training and Research Hospital, University of Health Science, 07100 Antalya, Turkiye. FAU - Bilgin, Akin AU - Bilgin A AUID- ORCID: 0009-0009-8460-084X AD - Department of Orthopaedics and Traumatology, Antalya Training and Research Hospital, University of Health Science, 07100 Antalya, Turkiye. FAU - Yildirim, Caner AU - Yildirim C AUID- ORCID: 0009-0000-9128-718X AD - Department of Orthopaedics and Traumatology, Antalya Training and Research Hospital, University of Health Science, 07100 Antalya, Turkiye. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705344 OTO - NOTNLM OT - MINAR reconstruction OT - Rockwood classification OT - acromioclavicular joint separation OT - coracoclavicular fixation OT - football OT - retrospective analysis OT - return to sport OT - vertical reduction loss COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:39 PHST- 2026/07/19 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - jcm15207907 [pii] AID - jcm-15-07907 [pii] AID - 10.3390/jcm15207907 [doi] PST - epublish SO - J Clin Med. 2026 Oct 13;15(20):7907. doi: 10.3390/jcm15207907. PMID- 42941416 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Experiences in the Management of Type A Aortic Dissection Following Cardiovascular Interventions: A Case Series Report. LID - 10.3390/jcm15207901 [doi] LID - 7901 AB - Background: Cardiovascular interventions such as transcatheter aortic valve replacement (TAVR) and transcatheter edge-to-edge repair (TEER) have become standard options for high-risk patients with valvular heart disease. However, iatrogenic type A aortic dissection (AAD) remains a rare but life-threatening complication, and its optimal management is controversial. Case presentation: We present four patients who developed AAD following cardiovascular interventions at our institution between 2020 and 2024, including three after TAVR and one after TEER. Three patients underwent the Bentall procedure (each requiring removal of the pre-existing transcatheter valve, and one with concomitant coronary artery bypass grafting), and one underwent aortic sinus repair with ascending aorta and total arch replacement using a frozen elephant trunk, together with removal of the previously implanted TEER clip and mitral valve replacement. One patient recovered and was discharged home, another was transferred back to the cardiology department and was discharged home with no sequelae, and two left the hospital against medical advice while still in critical condition, both of whom subsequently died-one 2 days after discharge and the other immediately upon arriving home. Conclusions: Our experience indicates that patients with cardiovascular intervention-related AAD are at particularly high risk owing to pre-existing cardiac dysfunction and deterioration of other organ functions. In such patients, emergency surgical rescue can be life-saving, and the Bentall procedure proved feasible in this selected cohort; however, surgery in this setting is more complex than in routine cases. Careful evaluation, individualized surgical planning, and meticulous perioperative management are essential to improve outcomes. FAU - He, Lile AU - He L AD - Department of Cardiovascular Surgery, The Second Xiangya Hospital, Central South University, Changsha 410011, China. FAU - Zhang, Zhiwei AU - Zhang Z AD - Department of Cardiovascular Surgery, The Second Xiangya Hospital, Central South University, Changsha 410011, China. FAU - Tang, Tao AU - Tang T AD - Department of Cardiovascular Surgery, The Second Xiangya Hospital, Central South University, Changsha 410011, China. LA - eng GR - 82300463/National Natural Science Foundation of China/ GR - 2024JJ6562/Natural Science Foundation of Hunan Province/ GR - 2025JJ60831/Natural Science Foundation of Hunan Province/ PT - Case Reports PT - Journal Article DEP - 20261013 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705728 OTO - NOTNLM OT - Bentall procedure OT - TAVR OT - TEER OT - aortic dissection OT - cardiovascular intervention OT - case report OT - iatrogenic aortic dissection COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:39 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - jcm15207901 [pii] AID - jcm-15-07901 [pii] AID - 10.3390/jcm15207901 [doi] PST - epublish SO - J Clin Med. 2026 Oct 13;15(20):7901. doi: 10.3390/jcm15207901. PMID- 42941414 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Comparison of Internal Validation Strategies for Estimating Machine Learning Model Performance in One-Year Mortality Prediction in Hepatocellular Carcinoma. LID - 10.3390/jcm15207899 [doi] LID - 7899 AB - Background: Machine learning (ML) performance estimates in hepatocellular carcinoma (HCC) may depend on internal validation strategy, especially in small datasets. Aim: To develop models for one-year mortality prediction and compare performance, calibration, decision-analytic performance, and uncertainty estimates using a conventional train-test split and nested cross-validation (nCV). Methods: We analyzed a public dataset of 165 patients with HCC, including 63 one-year deaths. Random Forest (RF), XGBoost, and CatBoost were developed using fold-specific preprocessing, cost-sensitive learning, Optuna-based hyperparameter optimization, and training-based threshold selection. L2-penalized logistic regression provided a conventional benchmark. Models were evaluated using a stratified 80/20 split and five-fold nCV with five inner folds. Six stratified splits assessed partition variability, and unweighted models assessed the effect of class weighting. Discrimination, threshold-dependent classification, calibration, and decision-analytic performance were assessed using ROC-AUC, PR-AUC, balanced accuracy, Matthew's correlation coefficient, Brier score, calibration curves, and decision-curve analysis. Confidence intervals used 2000 bootstrap resamples; SHAP was used for interpretation. Results: In the conventional split, RF and CatBoost achieved the highest test-set ROC-AUC (0.892 each), while RF achieved the highest PR-AUC (0.860). Under nCV, RF had the highest mean outer-fold ROC-AUC (0.788) and pooled out-of-fold ROC-AUC (0.784). XGBoost had the highest mean PR-AUC (0.673), whereas CatBoost had the highest pooled PR-AUC (0.648). RF had the lowest mean Brier score (0.188), followed by XGBoost (0.194) and CatBoost (0.203). Calibration varied across models, with pooled calibration slopes of 1.438, 0.691, and 0.427, respectively. Across six 80/20 splits, mean ROC-AUC values were 0.792, 0.769, and 0.761 for RF, XGBoost, and CatBoost, respectively, with substantial partition-dependent variation. Removing class weighting yielded broadly comparable performance. Bootstrap confidence intervals were narrower for pooled nCV predictions, but the evaluation samples differed in size (165 versus 33 patients), precluding attribution of this difference to validation strategy alone. Decision-curve analysis was exploratory because no clinically actionable threshold was prespecified. SHAP identified AFP, ALP, hemoglobin, albumin, performance status, and AST among the most influential RF predictors; the highest-ranked predictors were generally consistent across outer folds. Conclusions: nCV separated model development from evaluation and reduced reliance on a single small test set. RF was selected by the prespecified mean outer-fold ROC-AUC criterion, but differences among tree-based models were small and no clear discrimination advantage over penalized logistic regression was demonstrated. External validation is needed before clinical use. FAU - Kucukakcali, Zeynep AU - Kucukakcali Z AUID- ORCID: 0000-0001-7956-9272 AD - Department of Biostatistics and Medical Informatics, Faculty of Medicine, Inonu University, 44280 Malatya, Turkiye. FAU - Akbulut, Sami AU - Akbulut S AUID- ORCID: 0000-0002-6864-7711 AD - Department of Biostatistics and Medical Informatics, Faculty of Medicine, Inonu University, 44280 Malatya, Turkiye. AD - Department of Surgery and Liver Transplantation, Faculty of Medicine, Inonu University, 44280 Malatya, Turkiye. FAU - Sarici, Kemal Baris AU - Sarici KB AD - Department of Surgery and Liver Transplantation, Faculty of Medicine, Inonu University, 44280 Malatya, Turkiye. LA - eng GR - TSA-2026-5235/Inonu University/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706229 OTO - NOTNLM OT - SHAP OT - calibration OT - clinical prediction model OT - decision curve analysis OT - hepatocellular carcinoma OT - internal validation OT - machine learning OT - nested cross-validation OT - one-year mortality prediction COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:39 PHST- 2026/08/29 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - jcm15207899 [pii] AID - jcm-15-07899 [pii] AID - 10.3390/jcm15207899 [doi] PST - epublish SO - J Clin Med. 2026 Oct 13;15(20):7899. doi: 10.3390/jcm15207899. PMID- 42941406 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Operative Indications for Lateral Compression Type 1 Pelvic Ring Injuries: A Systematic Review of Reported Selection Criteria. LID - 10.3390/jcm15207891 [doi] LID - 7891 AB - Background/Objectives: Lateral compression type 1 (LC-1) injuries range from stable patterns to injuries whose instability is not evident on initial imaging. How published studies define the criteria for operative fixation has not been characterized. Methods: We systematically reviewed studies reporting operative-selection criteria for LC-1 injuries. MEDLINE and Embase were searched (last update 14 August 2026) without date or language filters; English-language full text was required for eligibility, and dedicated fragility-fracture cohorts were excluded. Reports of the same or nested cohorts were linked into study-level units. Each unit was coded for indication category, assessment method, quantitative threshold, and selection-rule reconstructibility (whether the assessment, positive rule, and link to the operation could be reconstructed). A second clinician independently recoded three of these fields for all units. Registration (retrospective): OSF, 10.17605/OSF.IO/WSDGX. Results: Thirty-seven eligible reports contributed 29 study-level units. Selection was multifactorial in 15 units (51.7%), 16 (55.2%) used dynamic or stress assessment, and 18 (62.1%) reported a quantitative threshold, most often approximately 1 cm (10 units; 7 after collapsing four units from one institutional registry). Only 6 units (20.7%) met the prespecified definition of a fully reconstructible operative-selection rule. Of the remaining 23, two, by design, had no ascertainable clinical selection pathway; the other 21 all lacked a reconstructible positive rule (14 units), a clear criterion-to-treatment linkage (16), or both, whereas the assessment itself was non-reconstructible in only two. Inter-coder agreement for selection-rule reconstructibility was 89.7% (26/29). Conclusions: Operative indications for LC-1 injuries remain heterogeneous and incompletely operationalized. What to assess was almost always identifiable; reconstruction failed after it. Studies should report how instability is elicited, how a positive test is defined, and what that result caused. FAU - Lee, Jaeheon AU - Lee J AUID- ORCID: 0000-0001-6018-0451 AD - Department of Orthopedic Surgery, Saeson Hospital, Daejeon 34908, Republic of Korea. AD - Department of Medical Sciences, Graduate School of Ajou University, Suwon 16499, Republic of Korea. FAU - Song, Hyung Keun AU - Song HK AUID- ORCID: 0000-0001-5486-2560 AD - Department of Orthopaedic Surgery, Ajou University School of Medicine, Suwon 16499, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705429 OTO - NOTNLM OT - LC-1 OT - examination under anesthesia OT - lateral compression OT - operative indication OT - pelvic ring injury OT - reporting standards OT - stress radiography OT - systematic review OT - treatment selection COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:39 PHST- 2026/08/18 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - jcm15207891 [pii] AID - jcm-15-07891 [pii] AID - 10.3390/jcm15207891 [doi] PST - epublish SO - J Clin Med. 2026 Oct 13;15(20):7891. doi: 10.3390/jcm15207891. PMID- 42941401 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Precision Therapeutics for Monogenic Epilepsies: From Molecular Mechanisms to Disease Modification in Developmental and Epileptic Encephalopathies and Neurodevelopmental Disorders with Epilepsy. LID - 10.3390/jcm15207886 [doi] LID - 7886 AB - Background: Developmental and epileptic encephalopathies (DEEs) and neurodevelopmental disorders with epilepsy (NDD-E) are etiologically heterogeneous conditions historically treated with syndrome-based, symptomatic approaches. Advances in genomic sequencing have identified monogenic causes across more than 900 genes, creating opportunities for mechanism-informed and disease-modifying precision therapies. Methods: For this narrative review, PubMed/MEDLINE and ClinicalTrials.gov were searched through September 2026 using disorder-, gene-, mechanism-, and therapeutic-modality-specific terms to identify mechanism-informed and disease-modifying therapies for monogenic DEEs and NDD-E. Results: Pathogenic mechanisms include gain- or loss-of-function ion-channel abnormalities, altered neurotransmitter receptor/transporter function, impaired synaptic signaling, dysregulated intracellular pathways, metabolic deficiencies, protein misfolding, and transcriptional or epigenetic dysregulation. Across genes such as SCN1A, SCN2A, SCN8A, KCNQ2, KCNT1, KCNA2, CACNA1A, GABA-receptor subunits, SLC6A1, GRIN genes, SYNGAP1, STXBP1, DNM1, SHANK3, TSC1/TSC2, GNAO1, UBE3A, MECP2, and TCF4, therapeutic strategies range from repurposed and mechanism-targeted pharmacologic agents (e.g., sodium- and potassium-channel modulators, mTOR inhibitors) to antisense oligonucleotides, RNA interference, gene replacement or augmentation, transcriptional activation, and genome editing. Several programs have advanced from preclinical proof of concept into early- and late-phase human trials, with variant-specific functional classification increasingly guiding therapy selection, particularly distinguishing gain-of-function from loss-of-function disease. Conclusions: Precision therapeutics are shifting the treatment paradigm for monogenic DEEs and NDD-E from empiric seizure control toward mechanism-based targeting of dysregulated molecular pathways and gene-based disease-modifying therapies. Realizing this potential will require functional variant classification, delivery technologies capable of durable and cell-appropriate target engagement, and trial designs incorporating developmental and biomarker outcomes beyond seizure frequency alone to demonstrate genuine disease modification. FAU - Samanta, Debopam AU - Samanta D AUID- ORCID: 0000-0002-5154-8717 AD - Division of Child Neurology, Department of Pediatrics, University of Arkansas for Medical Sciences, 1 Children's Way, Little Rock, AR 72202, USA. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705553 OTO - NOTNLM OT - functional genomics OT - genotype-phenotype correlation OT - molecular diagnostics OT - personalized medicine OT - translational neuroscience COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:39 PHST- 2026/09/25 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - jcm15207886 [pii] AID - jcm-15-07886 [pii] AID - 10.3390/jcm15207886 [doi] PST - epublish SO - J Clin Med. 2026 Oct 13;15(20):7886. doi: 10.3390/jcm15207886. PMID- 42941395 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Perinatal and Early Respiratory Factors Associated with Invasive Mechanical Ventilation in Very Preterm Infants: A Retrospective Cohort Study from Kazakhstan. LID - 10.3390/jcm15207879 [doi] LID - 7879 AB - Background: Evidence on factors associated with the timing of invasive mechanical ventilation (IMV) among very preterm infants in Central Asia remains limited. Methods: This retrospective cohort study included infants born at 22-30 weeks gestation in two tertiary perinatal centers in Kazakhstan between 2021 and 2025. Analyses were restricted to 381 infants with complete data. IMV initiated during the first week of life was classified as early IMV, and IMV initiated after the first week as late IMV. Separate multivariable logistic regression models were constructed for any, early, and late IMV. Results: Any IMV was recorded in 191 infants (50.1%), including 132 (34.6%) with early IMV and 59 (15.5%) with late IMV. Early IMV was associated with gestational age 0.30 (aOR 4.39, 95% CI 1.97-9.77). Complete antenatal corticosteroid exposure was inversely associated with early IMV (aOR 0.38, 95% CI 0.20-0.72). Late IMV was associated with birth weight < 1000 g (aOR 3.51, 95% CI 1.47-8.35) and initial FiO(2) > 0.30 (aOR 2.12, 95% CI 1.02-4.39). An initial set CPAP >/= 12 cmH(2)O was recorded in 71.9% of infants and was associated with any IMV but not with early or late IMV separately. Conclusions: Early and late IMV were associated with different clinical patterns. These observational associations may support risk assessment but do not establish causal effects of respiratory-support practices. FAU - Alimukhamedov, Umid AU - Alimukhamedov U AD - Department of Neonatology, Asfendiyarov Kazakh National Medical University, Almaty 050012, Kazakhstan. AD - JSC "Scientific Center of Obstetrics, Gynecology and Perinatology", Almaty 050020, Kazakhstan. FAU - Bozhbanbayeva, Nishankul AU - Bozhbanbayeva N AUID- ORCID: 0000-0002-7487-2472 AD - Department of Neonatology, Asfendiyarov Kazakh National Medical University, Almaty 050012, Kazakhstan. FAU - Cetinkaya, Merih AU - Cetinkaya M AD - Division of Neonatology, Department of Pediatrics, Faculty of Medicine, Istanbul Atlas University, Istanbul 34408, Turkey. FAU - Tolykbayev, Talgat AU - Tolykbayev T AD - Zhambyl Regional Perinatal Center, Department of Health of the Akimat of Zhambyl Region, Taraz 080000, Kazakhstan. FAU - Sadykova, Altynay AU - Sadykova A AD - Department of Children's Diseases Named After Professor N.A. Barlybayeva, Asfendiyarov Kazakh National Medical University, Almaty 050012, Kazakhstan. FAU - Suieubekov, Bekzat AU - Suieubekov B AD - Department of Pediatric Anesthesiology and Intensive Care, Asfendiyarov Kazakh National Medical University, Almaty 050012, Kazakhstan. FAU - Alseitov, Ulykpan AU - Alseitov U AD - Children's Emergency Medicine Center, Almaty 050040, Kazakhstan. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705521 OTO - NOTNLM OT - CPAP OT - LISA OT - invasive mechanical ventilation OT - respiratory distress syndrome OT - very preterm infant COIS- The authors declare that they have no financial or non-financial competing interests relevant to this work. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:39 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - jcm15207879 [pii] AID - jcm-15-07879 [pii] AID - 10.3390/jcm15207879 [doi] PST - epublish SO - J Clin Med. 2026 Oct 13;15(20):7879. doi: 10.3390/jcm15207879. PMID- 42941385 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - CT-Derived Adipose Tissue Biomarkers in Coronary and Carotid Atherosclerosis: A Systematic Review of Dual-Energy, Spectral, and Photon-Counting CT. LID - 10.3390/jcm15207870 [doi] LID - 7870 AB - Background/Objectives: Advanced Computed Tomography (CT) enables multiparametric characterization of local perivascular and regional or systemic adipose tissue, potentially providing markers of vascular inflammation and cardiometabolic remodeling beyond conventional assessment of the vessel lumen and plaque. However, evidence remains fragmented across CT technologies, adipose depots and vascular territories. This systematic review evaluated human studies of adipose-tissue biomarkers derived from dual-layer spectral-detector CT (DLSCT), dual-source dual-energy CT (DS-DECT), rapid kVp-switching dual-energy CT (rsDECT), and photon-counting CT (PCCT) in coronary and carotid atherosclerosis. Methods: PubMed and Scopus were systematically searched for English-language human studies published from 1 January 2020, to 13 September 2026, supplemented by backward and forward citation searching of eligible studies and reference-list screening of relevant reviews. Two reviewers independently performed study selection and risk-of-bias assessment. Because of clinical, technical, and methodological heterogeneity, findings were synthesized narratively without meta-analysis. Results: Nineteen reports representing 15 likely independent study clusters met the inclusion criteria. DLSCT-derived pericoronary adipose-tissue attenuation; DECT-derived carotid perivascular adipose-tissue parameters; PCCT-derived adipose attenuation, volume, and radiomic features; and spectral measures of hepatic or visceral adiposity were associated with coronary artery disease, stenosis severity, high-risk plaque features, coronary calcification, or symptomatic carotid disease. Reported discrimination estimates were heterogeneous across populations, endpoints, and model structures and were predominantly derived from unvalidated development samples. Conclusions: Advanced CT-derived adipose-tissue biomarkers may complement conventional assessment of the vessel lumen and plaque by characterizing local vessel-adipose interactions and systemic cardiometabolic phenotype. However, current measurements are protocol- and platform-dependent, and clinical utility has not been established. Standardized measurement pipelines, biological validation, and prospective multicenter studies are required before routine clinical implementation. FAU - Bucolo, Giuseppe Mauro AU - Bucolo GM AUID- ORCID: 0000-0002-2076-5532 AD - Diagnostic and Interventional Radiology Unit, BIOMORF Department, University Hospital "AOU G. Martino", 98124 Messina, Italy. AD - Departement of Radiology, I.R.C.C.S. Centro Neurolesi Bonino Pulejo, 98124 Messina, Italy. FAU - Gaeta, Michele AU - Gaeta M AD - Diagnostic and Interventional Radiology Unit, BIOMORF Department, University Hospital "AOU G. Martino", 98124 Messina, Italy. FAU - Blandino, Alfredo AU - Blandino A AD - Diagnostic and Interventional Radiology Unit, BIOMORF Department, University Hospital "AOU G. Martino", 98124 Messina, Italy. FAU - Vinci, Sergio Lucio AU - Vinci SL AUID- ORCID: 0000-0002-7443-8270 AD - Neuroradiology Unit, Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University Hospital "AOU G. Martino", 98125 Messina, Italy. FAU - Cau, Riccardo AU - Cau R AUID- ORCID: 0000-0002-7910-1087 AD - Department of Radiology, Azienda Ospedaliero-Universitaria (A.O.U.) di Cagliari, 09124 Cagliari, Italy. FAU - Booz, Christian AU - Booz C AD - Department of Diagnostic and Interventional Radiology, University Hospital of Frankfurt, 60596 Frankfurt, Germany. FAU - Vogl, Thomas Josef AU - Vogl TJ AUID- ORCID: 0000-0001-5218-1075 AD - Department of Diagnostic and Interventional Radiology, University Hospital of Frankfurt, 60596 Frankfurt, Germany. FAU - Koch, Vitali AU - Koch V AUID- ORCID: 0000-0001-6915-5906 AD - Department of Diagnostic and Interventional Radiology, University Hospital of Frankfurt, 60596 Frankfurt, Germany. FAU - Gruenewald, Leon David AU - Gruenewald LD AUID- ORCID: 0000-0001-7959-5008 AD - Department of Diagnostic and Interventional Radiology, University Hospital of Frankfurt, 60596 Frankfurt, Germany. FAU - Yel, Ibrahim AU - Yel I AUID- ORCID: 0000-0002-3894-4782 AD - Department of Diagnostic and Interventional Radiology, University Hospital of Frankfurt, 60596 Frankfurt, Germany. FAU - Saba, Luca AU - Saba L AUID- ORCID: 0000-0003-2870-3771 AD - Department of Radiology, Azienda Ospedaliero-Universitaria (A.O.U.) di Cagliari, 09124 Cagliari, Italy. FAU - Mazziotti, Silvio AU - Mazziotti S AUID- ORCID: 0000-0001-9866-569X AD - Diagnostic and Interventional Radiology Unit, BIOMORF Department, University Hospital "AOU G. Martino", 98124 Messina, Italy. FAU - D'Angelo, Tommaso AU - D'Angelo T AUID- ORCID: 0000-0003-0004-6378 AD - Diagnostic and Interventional Radiology Unit, BIOMORF Department, University Hospital "AOU G. Martino", 98124 Messina, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705383 OTO - NOTNLM OT - adipose tissue OT - angiography OT - atherosclerosis OT - cardiac imaging technique OT - dual-energy CT OT - epicardial adipose tissue OT - photon counting CT OT - radiomics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:39 PHST- 2026/08/12 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - jcm15207870 [pii] AID - jcm-15-07870 [pii] AID - 10.3390/jcm15207870 [doi] PST - epublish SO - J Clin Med. 2026 Oct 12;15(20):7870. doi: 10.3390/jcm15207870. PMID- 42941383 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - Associations of Thrombocytopenia and Subthreshold Platelet Counts with Clinical Outcomes in Intensive Care Patients: A Prospective Cohort Study. LID - 10.3390/jcm15207869 [doi] LID - 7869 AB - Background/Objectives: Thrombocytopenia (TP) is common in critically ill patients, but the significance of its severity, timing, persistence, and admission subthreshold platelet count (PC) remains uncertain. We evaluated these associations with mortality in a medical ICU. Methods: In this prospective cohort study, 280 adults admitted to a tertiary medical ICU (September 2023-August 2024) were followed until ICU discharge or death. TP was defined as a PC < 150 x 10(9)/L, and admission STP as a first ICU PC of 150-199 x 10(9)/L, irrespective of later platelet course. Outcomes included mortality (ICU, hospital, 28-day, 90-day), organ support, and major complications. Results: TP occurred in 118 patients (42.1%), with higher crude mortality at all time points (all p < 0.001). In the lagged time-dependent Cox analysis, ICU-acquired TP was associated with ICU mortality in the minimally adjusted model (HR 1.93, CI 1.12-3.35; p = 0.018) but not after adjustment for invasive MV (aHR 1.31, CI 0.72-2.40; p = 0.372). Admission TP was not significantly associated with ICU mortality in the adjusted model (aHR 1.22, CI 0.74-2.00; p = 0.436). Admission PC showed limited discrimination (AUC 0.583, CI 0.508-0.657). No statistically significant difference was found between admission STP and PC >/= 200 x 10(9)/L (exploratory comparison, wide CIs). Conclusions: TP was associated with disease severity and worse crude outcomes, but adjusted longitudinal analyses did not establish an independent association with ICU mortality. No statistically significant difference was found for admission STP versus PC >/= 200 x 10(9)/L in this exploratory comparison; this should not be interpreted as clinical equivalence or as indicating no prognostic value. FAU - Deveci, Kamil AU - Deveci K AUID- ORCID: 0000-0002-9108-4769 AD - Division of Intensive Care Unit, Department of Internal Medicine, Kayseri City Education and Research Hospital, 38020 Kayseri, Turkiye. FAU - Boran, Maruf AU - Boran M AUID- ORCID: 0000-0002-2494-9152 AD - Division of Intensive Care Unit, Department of Internal Medicine, Kayseri City Education and Research Hospital, 38020 Kayseri, Turkiye. FAU - Bahar, Kubra Elif AU - Bahar KE AUID- ORCID: 0009-0008-2167-6380 AD - Department of Internal Medicine, Kayseri City Education and Research Hospital, 38020 Kayseri, Turkiye. FAU - Saridag, Fatma AU - Saridag F AUID- ORCID: 0009-0007-7988-0352 AD - Department of Internal Medicine, Kayseri City Education and Research Hospital, 38020 Kayseri, Turkiye. FAU - Yildizhan, Esra AU - Yildizhan E AD - Division of Hematology, Department of Internal Medicine, Kayseri City Education and Research Hospital, 38020 Kayseri, Turkiye. LA - eng PT - Journal Article DEP - 20261012 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705574 OTO - NOTNLM OT - critical care OT - mortality OT - thrombocytopenia COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:39 PHST- 2026/07/22 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:39 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - jcm15207869 [pii] AID - jcm-15-07869 [pii] AID - 10.3390/jcm15207869 [doi] PST - epublish SO - J Clin Med. 2026 Oct 12;15(20):7869. doi: 10.3390/jcm15207869. PMID- 42941382 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - Patient Selection and Reoperation After Cement-Augmented Pedicle Screw Fixation in the Thoracolumbar Spine: A Single-Center Cohort. LID - 10.3390/jcm15207867 [doi] LID - 7867 AB - Background/Objectives: Poor bone quality compromises pedicle screw fixation, and cement augmentation of fenestrated screws improves fixation at the screw-bone interface. Whether low interface-failure rates translate into low all-cause reoperation rates in routine practice is not established. We characterized the institutional use of cement-augmented fenestrated pedicle screws, the retrospectively measured vertebral computed tomography (CT) attenuation of selected patients, and subsequent interface failure and reoperation. Methods: We reviewed all patients undergoing thoracolumbar pedicle screw fixation between January 2016 and June 2022 at one tertiary referral center. Vertebral attenuation was measured retrospectively in Hounsfield units (HU) at L1 through L4. Outcome proportions are reported with Wilson 95% confidence intervals. Comparisons across surgical indications used exact tests with Holm correction and were treated as exploratory. Cement leakage and other cement-specific safety events could not be ascertained and were not study outcomes. Results: Cement augmentation was used in 101 of 2285 screened patients (4.4%), and 68 met the criteria for the outcome cohort (median follow-up, 44.9 weeks). Median attenuation was 94 HU, and 35 of 53 measurable patients (66.0%) fell below 110 HU, whereas formal densitometry was documented in 3. Screw loosening and pullout each occurred in three patients (4.4%), and reoperation for any cause occurred in nine (13.2%). Reoperation was more frequent among deformity constructs (30.4%) than for trauma (6.1%) or oncologic disease (0%); omnibus exact p = 0.016, although this result did not remain significant after Holm correction (adjusted p = 0.10). Conclusions: Cement augmentation was applied selectively, and retrospective CT measurements identified low vertebral attenuation in most measurable patients, despite infrequent formal densitometry. Failure at the screw-bone interface was infrequent, whereas all-cause reoperation remained clinically relevant and varied by surgical context. This study describes the selection and outcomes after augmentation but cannot establish comparative effectiveness or the safety of the augmentation technique. FAU - Patel, Hershil A AU - Patel HA AUID- ORCID: 0009-0008-6820-2625 AD - Division of Spine Surgery, Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21021, USA. FAU - Suresh, Rohan I AU - Suresh RI AD - Division of Spine Surgery, Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21021, USA. FAU - Chiu, Anthony K AU - Chiu AK AD - Division of Spine Surgery, Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21021, USA. FAU - Patel, Sapan AU - Patel S AUID- ORCID: 0000-0003-4900-4187 AD - Division of Spine Surgery, Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21021, USA. FAU - Khanna, Shivam AU - Khanna S AD - Division of Spine Surgery, Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21021, USA. FAU - Mixa, Joseph AU - Mixa J AD - Division of Spine Surgery, Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21021, USA. FAU - Prakash, Hans AU - Prakash H AD - Division of Spine Surgery, Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21021, USA. FAU - Sahai, Amil AU - Sahai A AUID- ORCID: 0000-0002-1501-2771 AD - Division of Spine Surgery, Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21021, USA. FAU - Patel, Akul AU - Patel A AD - Division of Spine Surgery, Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21021, USA. FAU - Padovano, Alexander AU - Padovano A AD - Division of Spine Surgery, Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21021, USA. FAU - Bivona, Louis J AU - Bivona LJ AD - Division of Spine Surgery, Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21021, USA. FAU - Cavanaugh, Daniel L AU - Cavanaugh DL AD - Division of Spine Surgery, Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21021, USA. FAU - Koh, Eugene Y AU - Koh EY AUID- ORCID: 0000-0002-7905-1761 AD - Division of Spine Surgery, Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21021, USA. FAU - Ludwig, Steven C AU - Ludwig SC AUID- ORCID: 0000-0002-3962-5724 AD - Division of Spine Surgery, Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21021, USA. FAU - Jauregui, Julio J AU - Jauregui JJ AUID- ORCID: 0000-0002-7878-0241 AD - Division of Spine Surgery, Department of Orthopaedics, University of Maryland School of Medicine, Baltimore, MD 21021, USA. LA - eng PT - Journal Article DEP - 20261012 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705460 OTO - NOTNLM OT - Hounsfield unit OT - bone quality OT - cement augmentation OT - opportunistic computed tomography OT - pedicle screws OT - reoperation OT - thoracolumbar COIS- The following authors have disclosed potential conflicts of interest: J.J.-Children: editorial or governing board; D.C.-Alphatec Spine: paid consultant; stock or stock options; E.K.-Alphatec Spine: stock or stock options; S.L.-AAOS: board or committee member; Alphatec Spine: IP royalties; stock or stock options; American Board of Orthopaedic Surgery, Inc.: board or committee member; American Orthopedic Association: board or committee member; AO Spine North America Spine Fellowship Support: research support; ASIP, ISD: stock or stock options; Atlas Spine: IP royalties; Baxter: research support; Cervical Spine Research Society: board or committee member; Contemporary Spine Surgery: editorial or governing board; DePuy, A Johnson & Johnson Company: IP royalties; LSRS: board or committee member; MDC: stock or stock options; Nuvasive: IP royalties; paid consultant; paid presenter or speaker; stock or stock options; OMEGA: research support; Smiss: board or committee member; Stryker: IP royalties; The Spine Journal: editorial or governing board. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:38 PHST- 2026/08/26 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - jcm15207867 [pii] AID - jcm-15-07867 [pii] AID - 10.3390/jcm15207867 [doi] PST - epublish SO - J Clin Med. 2026 Oct 12;15(20):7867. doi: 10.3390/jcm15207867. PMID- 42941380 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - Operative Versus Non-Operative Management for Pancreatic Trauma: An Umbrella Review of Systematic Reviews and Meta-Analyses. LID - 10.3390/jcm15207865 [doi] LID - 7865 AB - Background/Objectives: Pancreatic trauma is uncommon but highly morbid, and management has shifted toward non-operative management (NOM) with endoscopic and percutaneous adjuncts. Existing systematic reviews report heterogeneous findings, and no umbrella review has been published. We therefore synthesized this evidence, comparing operative management (OM) with NOM. Methods: MEDLINE, Embase, and the Cochrane Library were searched through 3 September 2026, following PRISMA 2020 and PRIOR (PROSPERO CRD420261495303). Eligible studies were systematic reviews comparing OM with NOM in pancreatic trauma. Two reviewers independently performed selection, extraction, AMSTAR 2, and ROBIS appraisal. Findings were synthesized narratively, separately for children and adults, with blunt injury as the primary comparison. Results: Six reviews were included (three pediatric, two adult, one mixed). In children, no review demonstrated a statistically significant difference in mortality (odds ratio [OR] for NOM vs. OM, 0.96; 95% CI, 0.42-2.22); NOM succeeded in 87% and was associated with more pseudocysts (OR, 3.05; 95% CI, 1.84-5.05; OR, 12.46 in grade III or higher injury), two-thirds managed without surgery, and with less fistula (OR, 0.27; 95% CI, 0.09-0.82). Adult estimates were crude and highly imprecise (mortality risk ratio for OM vs. NOM, 0.67; 95% CI, 0.04-9.91). Pediatric reviews overlapped heavily (corrected covered area, 38.3%); all six had critically low confidence and high risk of bias. Conclusions: The evidence does not allow the two strategies to be judged equivalent in either population. It is confounded by indication and concentrated in overlapping pediatric cohorts; the key unanswered question concerns stable patients with blunt ductal injury, stratified by ductal location. FAU - Min, Soon Ki AU - Min SK AUID- ORCID: 0000-0001-5966-9030 AD - Department of Trauma Surgery, Gachon University Gil Medical Center, Incheon 21565, Republic of Korea. FAU - Lee, Mina AU - Lee M AUID- ORCID: 0000-0002-9725-8993 AD - Department of Trauma Surgery, Gachon University Gil Medical Center, Incheon 21565, Republic of Korea. AD - Department of Traumatology, Gachon University College of Medicine, Incheon 21565, Republic of Korea. FAU - Kim, Youngmin AU - Kim Y AUID- ORCID: 0000-0003-1980-8889 AD - Department of Trauma Surgery, Gachon University Gil Medical Center, Incheon 21565, Republic of Korea. AD - Department of Traumatology, Gachon University College of Medicine, Incheon 21565, Republic of Korea. FAU - Choi, Kang Kook AU - Choi KK AUID- ORCID: 0000-0003-2650-4330 AD - Department of Trauma Surgery, Gachon University Gil Medical Center, Incheon 21565, Republic of Korea. AD - Department of Traumatology, Gachon University College of Medicine, Incheon 21565, Republic of Korea. FAU - Yu, Byungchul AU - Yu B AUID- ORCID: 0000-0002-2444-2343 AD - Department of Trauma Surgery, Gachon University Gil Medical Center, Incheon 21565, Republic of Korea. AD - Department of Traumatology, Gachon University College of Medicine, Incheon 21565, Republic of Korea. FAU - Lee, Gil Jae AU - Lee GJ AUID- ORCID: 0000-0002-7943-5933 AD - Department of Trauma Surgery, Gachon University Gil Medical Center, Incheon 21565, Republic of Korea. AD - Department of Traumatology, Gachon University College of Medicine, Incheon 21565, Republic of Korea. FAU - Kang, Wu Seong AU - Kang WS AUID- ORCID: 0000-0003-0136-4458 AD - Department of Trauma Surgery, Gachon University Gil Medical Center, Incheon 21565, Republic of Korea. AD - Department of Traumatology, Gachon University College of Medicine, Incheon 21565, Republic of Korea. LA - eng GR - RS-2024-00509257 and IITP-2026-RS-2024-00438239/Ministry of Science and ICT/ GR - RS-2025-02220492/Ministry of Health and Welfare/ GR - RS-2026-25602618/Ministry of Science and ICT/ PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705537 OTO - NOTNLM OT - non-operative management OT - pancreatic fistula OT - pancreatic injury OT - pancreatic pseudocyst OT - umbrella review COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:38 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - jcm15207865 [pii] AID - jcm-15-07865 [pii] AID - 10.3390/jcm15207865 [doi] PST - epublish SO - J Clin Med. 2026 Oct 12;15(20):7865. doi: 10.3390/jcm15207865. PMID- 42941378 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - Short-Term Outcomes After Corticosteroid Injection in Patients with Clinically Diagnosed Subacromial Pain Suggestive of Bursitis. LID - 10.3390/jcm15207863 [doi] LID - 7863 AB - Background: The current diagnostic paradigm for clinically suspected subacromial bursitis is based on clinical history and physical examination. However, clinical signs frequently overlap with other pathologies. This study investigated short-term outcomes following the currently employed clinical criteria by comparing patients who met these criteria and received an injection with those who were offered but refused an injection. Methods: From July 2024 to April 2025, patients with symptoms of clinically suspected subacromial bursitis were enrolled and offered a corticosteroid injection. Patients were grouped based on whether they agreed to and received an injection or not. Range of motion (ROM) and patient-reported outcomes (PROs) were collected at the initial visit and Visual Analog Scale (VAS), Single Assessment Numeric Evaluation (SANE), and Disabilities of the Arm, Shoulder and Hand (DASH) questionnaire scores were collected using questionnaires emailed 2 weeks after the initial visit. This was a prospective, non-randomized observational study with treatment determined by patient preference. VAS and DASH were the primary outcomes. Results: 98 patients were enrolled and 73 patients received a corticosteroid injection. Follow-up PROs were completed by 47 injection patients and 18 non-injected patients. For injection patients, VAS scores improved from 5.3 +/- 2.5 to 3.8 +/- 2.8 (p < 0.001), and DASH scores improved from 42.2 +/- 17.7 to 27.0 +/- 21.7 (p < 0.001). Patients without injection also improved in VAS (5.1 +/- 2.7 to 3.9 +/- 2.7, p = 0.015) and DASH scores (38.3 +/- 23.8 to 27.0 +/- 18.8, p = 0.006). Between-group differences in changes in VAS, DASH, and affected-side SANE scores were not statistically significant (p = 0.661, p = 0.405, and p = 0.512, respectively). Conclusions: No statistically significant differences in pain or affected-shoulder function between groups were detected at approximately 2 weeks in this small, non-randomized study. These findings do not establish equivalence or determine whether clinical criteria identify patients who benefit from injection. Further studies should assess whether clinical and imaging findings improve patient selection. FAU - Schroen, Christoph A AU - Schroen CA AUID- ORCID: 0009-0001-5715-4778 AD - Department of Orthopaedic Surgery, Icahn School of Medicine, Mount Sinai, 1468 Madison Avenue, New York, NY 10029, USA. AD - Medical Faculty Heidelberg, Heidelberg University, 69120 Heidelberg, Germany. FAU - Yu, Alexander AU - Yu A AUID- ORCID: 0000-0002-7246-2269 AD - Department of Orthopaedic Surgery, Icahn School of Medicine, Mount Sinai, 1468 Madison Avenue, New York, NY 10029, USA. FAU - Duey, Akiro H AU - Duey AH AD - Department of Orthopaedic Surgery, Icahn School of Medicine, Mount Sinai, 1468 Madison Avenue, New York, NY 10029, USA. FAU - Guttikonda, Sri AU - Guttikonda S AD - Department of Orthopaedic Surgery, Icahn School of Medicine, Mount Sinai, 1468 Madison Avenue, New York, NY 10029, USA. FAU - Li, Michael AU - Li M AD - Department of Orthopaedic Surgery, Icahn School of Medicine, Mount Sinai, 1468 Madison Avenue, New York, NY 10029, USA. FAU - Hausman, Richard AU - Hausman R AUID- ORCID: 0009-0004-6247-6909 AD - Department of Orthopaedic Surgery, Icahn School of Medicine, Mount Sinai, 1468 Madison Avenue, New York, NY 10029, USA. FAU - Bernstein, Jordan AU - Bernstein J AD - Department of Orthopaedic Surgery, Icahn School of Medicine, Mount Sinai, 1468 Madison Avenue, New York, NY 10029, USA. FAU - Ranson, William A AU - Ranson WA AUID- ORCID: 0000-0001-8955-7080 AD - Department of Orthopaedic Surgery, Icahn School of Medicine, Mount Sinai, 1468 Madison Avenue, New York, NY 10029, USA. FAU - Hausman, Michael R AU - Hausman MR AD - Department of Orthopaedic Surgery, Icahn School of Medicine, Mount Sinai, 1468 Madison Avenue, New York, NY 10029, USA. FAU - Cagle, Paul J AU - Cagle PJ AD - Department of Orthopaedic Surgery, Icahn School of Medicine, Mount Sinai, 1468 Madison Avenue, New York, NY 10029, USA. LA - eng PT - Journal Article DEP - 20261012 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705209 OTO - NOTNLM OT - bursitis OT - corticosteroid injection OT - range of motion OT - shoulder OT - shoulder pain OT - subacromial bursitis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:38 PHST- 2026/08/04 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - jcm15207863 [pii] AID - jcm-15-07863 [pii] AID - 10.3390/jcm15207863 [doi] PST - epublish SO - J Clin Med. 2026 Oct 12;15(20):7863. doi: 10.3390/jcm15207863. PMID- 42941374 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - Robot-Assisted Radical Cystectomy with Intracorporeal Urinary Diversion: Perioperative Management and Operative Technique. LID - 10.3390/jcm15207858 [doi] LID - 7858 AB - Radical cystectomy with bilateral pelvic lymphadenectomy and urinary diversion remains the standard of care for muscle-invasive bladder cancer and selected high-risk non-muscle-invasive disease. The procedure carries substantial morbidity, in part because the typical patient is elderly and burdened by cardiorespiratory comorbidity. Robot-assisted radical cystectomy (RARC) was developed to attenuate this morbidity, and RARC with intracorporeal urinary diversion (iRARC) avoids the laparotomy that earlier randomized trials required for extracorporeal reconstruction. In this technical narrative review, we present a single-institution operative approach to iRARC, distinguishing institutional practice from evidence-based recommendations. We describe patient selection, preoperative optimization and enhanced recovery, and provide a step-by-step account of our operative technique for male and female radical cystectomy, pelvic lymph node dissection template, and totally intracorporeal ileal conduit and orthotopic neobladder reconstruction. We also summarize the perioperative, oncological, and functional outcomes of iRARC, which support its role as a safe option with specific perioperative advantages in experienced, high-volume centers. FAU - Zillo, Cristofer AU - Zillo C AD - Department of Urology, Yale School of Medicine, 789 Howard Avenue, New Haven, CT 06520, USA. FAU - Shah, Ashti AU - Shah A AUID- ORCID: 0000-0001-8990-9509 AD - Department of Urology, Yale School of Medicine, 789 Howard Avenue, New Haven, CT 06520, USA. FAU - Perez-Londono, Agustin AU - Perez-Londono A AD - Department of Urology, Yale School of Medicine, 789 Howard Avenue, New Haven, CT 06520, USA. FAU - Li, Jinlei AU - Li J AUID- ORCID: 0000-0003-4026-2569 AD - Department of Anesthesia, Yale School of Medicine, 333 Cedar Street, P.O. Box 208051, New Haven, CT 06520, USA. FAU - Carr, Zyad J AU - Carr ZJ AUID- ORCID: 0000-0002-6970-3340 AD - Department of Anesthesia, Yale School of Medicine, 333 Cedar Street, P.O. Box 208051, New Haven, CT 06520, USA. AD - Pre-Surgical Evaluation Clinic, Yale New Haven Hospital, 20 York Street, New Haven, CT 06510, USA. FAU - Ghali, Fady AU - Ghali F AUID- ORCID: 0000-0001-7417-1599 AD - Department of Urology, Yale School of Medicine, 789 Howard Avenue, New Haven, CT 06520, USA. FAU - Tan, Wei Shen AU - Tan WS AUID- ORCID: 0000-0002-6119-4043 AD - Department of Urology, Yale School of Medicine, 789 Howard Avenue, New Haven, CT 06520, USA. AD - Division of Surgery and Interventional Science, Charles Bell House, 43-45 Foley Street, London W1W 7TS, UK. LA - eng PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705280 OTO - NOTNLM OT - bladder cancer OT - enhanced recovery OT - ileal conduit OT - orthotopic neobladder OT - radical cystectomy OT - robotic surgery OT - urinary diversion COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:38 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - jcm15207858 [pii] AID - jcm-15-07858 [pii] AID - 10.3390/jcm15207858 [doi] PST - epublish SO - J Clin Med. 2026 Oct 12;15(20):7858. doi: 10.3390/jcm15207858. PMID- 42941371 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - Heterogeneity in CT-Based Skeletal Muscle Mass Assessment and Sarcopenia Diagnosis Undermines Comparability in Hepatopancreatobiliary Surgery: A Scoping Review. LID - 10.3390/jcm15207856 [doi] LID - 7856 AB - Background/Objectives: Sarcopenia has emerged as an important prognostic factor in patients undergoing hepatopancreatobiliary (HPB) surgery. However, substantial heterogeneity in assessment methods and diagnostic thresholds limits comparability across studies. The aim of this review is to systematically characterize methodological variability in sarcopenia assessment within HPB surgical literature, quantify dispersion in reported cut-off values, and highlight key areas requiring standardization. Methods: A structured search of PubMed, Scopus, and Web of Science was conducted to identify studies evaluating sarcopenia in adult patients undergoing surgery for benign or malignant HPB diseases. The included studies assessed skeletal muscle mass with computed tomography (CT)-based measures, rather than sarcopenia as defined in contemporary consensus definitions. Data were extracted regarding imaging modality, anatomical landmarks, muscle indices, normalization strategies and diagnostic thresholds. Between-study variability in skeletal muscle index (SMI) thresholds was quantified using the coefficient of variation (CV). Results: Fifty-three studies comprising 11,613 patients met the inclusion criteria. Thirty-four studies assessed CT-defined low skeletal muscle mass using total skeletal muscle area at the third lumbar vertebral level normalized for height, whereas the remaining studies employed heterogeneous methodologies, including psoas-only measurements and alternative vertebral levels. Reported SMI cut-off values ranged from 36.2 to 53.5 cm(2)/m(2) in males and 29.6 to 41.1 cm(2)/m(2) in females. The CV for male and female cut-offs was 10.9% and 7.6%, respectively. Descriptively, lower CV values were observed in Western cohorts (male 6.5%; female 4.2%) than in Eastern cohorts (male 11.0%; female 9.0%); these values were not formally compared statistically. Considerable dispersion persisted irrespective of the threshold source. Conclusions: Despite the predominance of CT-derived L3 SMI assessment, marked methodological heterogeneity remains in HPB sarcopenia research. Variability in anatomical landmarks, normalization methods, and cut-off values limits reproducibility and constrains meta-analytic synthesis. Harmonization of reporting and threshold selection is essential to clarify the prognostic role of sarcopenia in HPB surgery. FAU - Papakonstantinou, Menelaos AU - Papakonstantinou M AUID- ORCID: 0000-0001-5030-7009 AD - First University Surgical Department, Papageorgiou General Hospital, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece. FAU - Chatzikomnitsa, Paraskevi AU - Chatzikomnitsa P AUID- ORCID: 0000-0001-7879-0426 AD - First University Surgical Department, Papageorgiou General Hospital, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece. FAU - Tsokkou, Sophia AU - Tsokkou S AUID- ORCID: 0009-0000-1703-6980 AD - First University Surgical Department, Papageorgiou General Hospital, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece. FAU - Gkaitatzi, Areti Danai AU - Gkaitatzi AD AUID- ORCID: 0000-0002-1245-7804 AD - First University Surgical Department, Papageorgiou General Hospital, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece. FAU - Giakoustidis, Dimitrios AU - Giakoustidis D AUID- ORCID: 0000-0002-6023-4744 AD - First University Surgical Department, Papageorgiou General Hospital, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece. FAU - Papadopoulos, Vasileios N AU - Papadopoulos VN AUID- ORCID: 0000-0002-1009-1685 AD - First University Surgical Department, Papageorgiou General Hospital, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece. FAU - Giakoustidis, Alexandros AU - Giakoustidis A AUID- ORCID: 0000-0002-3786-4609 AD - First University Surgical Department, Papageorgiou General Hospital, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece. LA - eng PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705426 OTO - NOTNLM OT - computed tomography OT - cut-off heterogeneity OT - hepatopancreatobiliary surgery OT - methodological heterogeneity OT - sarcopenia OT - skeletal muscle index COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:38 PHST- 2026/07/18 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - jcm15207856 [pii] AID - jcm-15-07856 [pii] AID - 10.3390/jcm15207856 [doi] PST - epublish SO - J Clin Med. 2026 Oct 12;15(20):7856. doi: 10.3390/jcm15207856. PMID- 42941362 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - Postoperative Outcomes of Robotic Versus Conventional Surgery in a Multi-Cancer Cohort: A Retrospective Cohort Study. LID - 10.3390/jcm15207846 [doi] LID - 7846 AB - Background/Objectives: Evidence on robotic surgery is largely procedure-specific and depends on the comparator. We examined associations with postoperative outcomes, prioritizing colorectal and uterine cancer and distinguishing laparoscopic from open comparators. Methods: This retrospective cohort included 2139 patients undergoing elective surgery for colorectal, uterine, prostate, or renal cancer at a tertiary referral center in 2019-2023. The primary analysis included 1232 colorectal or uterine cancer patients undergoing robotic (n = 462) or laparoscopic (n = 770) surgery. Generalized linear models adjusted for age, sex, cancer type, and T category/FIGO stage group; operative time and staffing were added in sensitivity analysis. Absolute differences were estimated by G-computation. The four-cancer pooled analysis was secondary. Results: Robotic surgery was associated with a shorter hospital stay (length-of-stay ratio 0.78; 95% CI 0.73-0.83; adjusted difference 2.34 days), which persisted with operative-time/staffing and calendar-year adjustment. Rehospitalization within 30 days after surgery was higher overall (adjusted odds ratio [aOR] 1.82; 95% CI 1.34-2.47), but differed by cancer: aOR 2.50 (95% CI 1.77-3.53) for colorectal and 0.38 (95% CI 0.16-0.90) for uterine cancer (interaction p < 0.001). Estimates for 30-day mortality, postoperative complications, and surgical site infection were imprecise. Conclusions: Rehospitalization differed in direction between these cancers, indicating that cancer type and comparator selection materially affect inference. These associations do not establish a platform-specific benefit or transferable bed-day savings. FAU - Cho, Jaehyeong AU - Cho J AD - Department of Research, Keimyung University Dongsan Medical Center, 1035 Dalgubeol-daero, Dalseo-gu, Daegu 42601, Republic of Korea. FAU - Kim, Hyoshuk AU - Kim H AD - Department of Digital Health, Samsung Advanced Institute for Health Sciences and Technology (SAIHST), Sungkyunkwan University, 115 Irwon-ro, Gangnam-gu, Seoul 06355, Republic of Korea. FAU - Cho, Chi-Heum AU - Cho CH AD - Department of Obstetrics and Gynecology, Keimyung University School of Medicine, 1095 Dalgubeol-daero, Dalseo-gu, Daegu 42601, Republic of Korea. LA - eng GR - RS-2025-02214164/Ministry of Health and Welfare/ GR - Keimyung University/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705545 OTO - NOTNLM OT - comparative effectiveness research OT - health resources OT - length of stay OT - minimally invasive surgical procedures OT - oncologic surgery OT - patient readmission OT - robotic surgical procedures COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:38 PHST- 2026/08/27 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - jcm15207846 [pii] AID - jcm-15-07846 [pii] AID - 10.3390/jcm15207846 [doi] PST - epublish SO - J Clin Med. 2026 Oct 12;15(20):7846. doi: 10.3390/jcm15207846. PMID- 42941361 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - Treatment Patterns and Survival in Older Patients with FIGO Stage IIB-IV Epithelial Ovarian, Fallopian Tube, and Primary Peritoneal Carcinomas. LID - 10.3390/jcm15207845 [doi] LID - 7845 AB - Background: Older adults with epithelial ovarian cancer (EOC) are underrepresented in prospective studies, and treatment selection is influenced by frailty, comorbidity, disease burden, and clinician assessment. We evaluated treatment patterns, operative outcomes, and survival associations in a single-center cohort of patients aged 70 years or older with FIGO stage IIB-IV EOC-spectrum malignancies. Methods: We screened 122 patients treated between January 2010 and December 2023 and included 97 patients after exclusions. The primary outcomes were progression-free survival (PFS) and overall survival (OS). PFS excluded seven patients recorded as receiving no treatment, whereas OS included all 97 patients. Kaplan-Meier methods were used to estimate PFS and OS, and overall log-rank tests were used to compare survival across age groups. Exploratory Cox proportional-hazards models included age, FIGO stage, and diagnosis era; post-baseline treatment completion and maintenance therapies were not included as fixed covariates because of potential guarantee-time bias and incomplete exposure dates. Results: The cohort comprised 44 patients aged 70-74 years, 38 aged 75-79 years, and 15 aged 80 years or older. Recorded treatment groups were primary debulking surgery (PDS; n = 46), NACT followed by recorded interval debulking surgery (NACT + IDS; n = 22), chemotherapy alone (n = 22), and no treatment (n = 7). Among the 90 patients in the PFS population, 61 experienced an event; among all 97 patients, 36 died. Median PFS was 22.0 months (95% CI, 16.0-24.0) and median OS was 51.5 months (95% CI, 38.0-not reached). Age-group log-rank p values were 0.684 for PFS and 0.248 for OS. The baseline Cox models were exploratory and showed no statistically significant association between age and PFS or OS after adjustment for FIGO stage and diagnosis era. Conclusions: This retrospective cohort does not establish that age, surgery, treatment completion, or maintenance therapy causes a survival difference. Survival estimates among patients aged 80 years or older were imprecise. Treatment comparisons should be interpreted as selected-cohort associations, and prospective studies incorporating validated geriatric, comorbidity, molecular, and time-dependent treatment information are needed. FAU - Zhang, Tingting AU - Zhang T AD - Department of Obstetrics and Gynecology, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Gongti South Road, Chaoyang District, Beijing 100020, China. FAU - Liang, Bing AU - Liang B AD - Department of Obstetrics and Gynecology, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Gongti South Road, Chaoyang District, Beijing 100020, China. FAU - Sang, Cuiqin AU - Sang C AD - Department of Obstetrics and Gynecology, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Gongti South Road, Chaoyang District, Beijing 100020, China. FAU - Guo, Zhengchen AU - Guo Z AD - Department of Obstetrics and Gynecology, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Gongti South Road, Chaoyang District, Beijing 100020, China. FAU - Li, Weihua AU - Li W AD - Department of Obstetrics and Gynecology, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Gongti South Road, Chaoyang District, Beijing 100020, China. FAU - Xu, Shuiqing AU - Xu S AD - Department of Obstetrics and Gynecology, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Gongti South Road, Chaoyang District, Beijing 100020, China. FAU - Li, Hua AU - Li H AUID- ORCID: 0000-0002-1172-6750 AD - Department of Obstetrics and Gynecology, Beijing Chaoyang Hospital, Capital Medical University, No. 8 Gongti South Road, Chaoyang District, Beijing 100020, China. LA - eng GR - QML20230307/Beijing Municipal Administration of Hospitals/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705369 OTO - NOTNLM OT - epithelial ovarian cancer OT - neoadjuvant chemotherapy OT - older adults OT - overall survival OT - primary debulking surgery OT - progression-free survival OT - retrospective cohort COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:38 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - jcm15207845 [pii] AID - jcm-15-07845 [pii] AID - 10.3390/jcm15207845 [doi] PST - epublish SO - J Clin Med. 2026 Oct 12;15(20):7845. doi: 10.3390/jcm15207845. PMID- 42941360 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - Association Between Breast Arterial Calcification on Mammography and Carotid Artery Stenosis on CT Angiography: A Retrospective Study in a Selected Cohort of Women Aged 60-65 Years. LID - 10.3390/jcm15207844 [doi] LID - 7844 AB - Background/Objectives: Breast arterial calcification (BAC) is an incidental mammographic finding that may be associated with systemic vascular disease. We investigated the association between mammographic BAC and carotid artery stenosis on cervical computed tomography angiography (CTA) in a clinically selected cohort of postmenopausal women aged 60-65 years. Methods: This retrospective, single-center study screened 3421 women aged 60-65 years who underwent both mammography and cervical CTA between November 2021 and May 2025. CTA examinations requested from the emergency department or cardiology clinics, examinations not performed within the same calendar year, and prespecified clinical exclusions were removed, leaving 180 women who underwent outpatient CTA for nonspecific symptoms. The median interval between examinations was 131 days. BAC was graded on a 0-3 scale, and carotid stenosis was assessed according to the North American Symptomatic Carotid Endarterectomy Trial (NASCET) criteria, using the greater degree of stenosis on either side as the patient-level value. BAC was analyzed as an ordinal variable, as a categorical variable, and using binary definitions of presence and severity. Multivariable models were adjusted for age, diabetes mellitus, hyperlipidemia, hypertension, and smoking. Results: Clinically significant stenosis (>/=50%) was present in 44 women (24.4%). Its prevalence increased from 5.4% in BAC grade 0 to 75.0% in grade 3 (p for trend < 0.001). Each one-grade increase in BAC was associated with clinically significant stenosis (adjusted odds ratio [aOR], 4.23; 95% confidence interval [CI], 2.50-8.03; p < 0.001). The corresponding aORs were 16.70 (95% CI, 4.99-73.27) for any BAC and 18.49 (95% CI, 5.93-70.46) for BAC grade >/=2. Adding BAC to the clinical model increased the area under the receiver operating characteristic curve (AUC) from 0.90 to 0.96 (DeLong p = 0.005); the optimism-corrected AUC was 0.95. Inter-reader agreement for BAC grading was substantial (weighted kappa = 0.79). Conclusions: In this selected cohort of women aged 60-65 years who underwent clinically indicated CTA, BAC was associated with carotid artery stenosis independently of conventional cardiovascular risk factors. These findings do not establish BAC as a screening or prediction tool and require prospective, population-based validation. FAU - Ayhan, Lacin Tatli AU - Ayhan LT AUID- ORCID: 0000-0002-4211-7958 AD - Department of Radiology, Faculty of Medicine, Sakarya University, 54290 Sakarya, Turkiye. FAU - Durmus, Mehmet Ali AU - Durmus MA AUID- ORCID: 0009-0008-8181-5987 AD - Department of Radiology, Sakarya Training and Research Hospital, 54100 Sakarya, Turkiye. FAU - Oguzman, Mehmet AU - Oguzman M AUID- ORCID: 0000-0002-6421-6236 AD - Department of Radiology, Sakarya Training and Research Hospital, 54100 Sakarya, Turkiye. FAU - Asil, Kiyasettin AU - Asil K AD - Department of Radiology, Sakarya Training and Research Hospital, 54100 Sakarya, Turkiye. FAU - Gunduz, Yasemin AU - Gunduz Y AD - Department of Radiology, Faculty of Medicine, Sakarya University, 54290 Sakarya, Turkiye. LA - eng PT - Journal Article DEP - 20261012 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13706212 OTO - NOTNLM OT - atherosclerosis OT - breast arterial calcification OT - carotid stenosis OT - computed tomography angiography OT - mammography OT - postmenopausal women COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:38 PHST- 2026/09/17 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - jcm15207844 [pii] AID - jcm-15-07844 [pii] AID - 10.3390/jcm15207844 [doi] PST - epublish SO - J Clin Med. 2026 Oct 12;15(20):7844. doi: 10.3390/jcm15207844. PMID- 42941359 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - Rethinking Postoperative Pain in the Era of Robotic Thoracic Surgery: A Surgeon's Perspective on Functional Recovery and Potential Oncologic Implications. LID - 10.3390/jcm15207843 [doi] LID - 7843 AB - Persistent postoperative pain remains an incompletely resolved problem after minimally invasive thoracic surgery, despite expectations that robot-assisted thoracic surgery (RATS) would meaningfully outperform video-assisted thoracoscopic surgery (VATS) in analgesia. This narrative review synthesizes evidence from randomized trials, cohort studies, and meta-analyses comparing acute and chronic pain outcomes between VATS and RATS, examines mechanisms and surgically modifiable risk factors underlying intercostal nerve injury and pain chronification, and evaluates the emerging concept of Return to Intended Oncologic Treatment (RIOT) as a perioperative endpoint linking pain control to oncologic recovery. Across randomized and propensity-matched comparisons, RATS has generally failed to demonstrate superior pain outcomes relative to VATS, and several studies suggest multiport robotic platforms may produce greater early pain than uniportal thoracoscopic approaches. Port number, operative time, and drainage duration emerge as modifiable surgical predictors of chronic pain, while inadequately controlled acute pain compromises functional recovery and is associated with delayed RIOT; direct evidence that pain independently influences RIOT or long-term survival remains limited, and this link should currently be regarded as a plausible, testable hypothesis rather than an established causal pathway. We conclude that, on the comparative evidence available to date, robotic instrumentation has not shown a consistent, clinically meaningful reduction in pain burden relative to VATS, and that pain management should be reframed as a shared surgical-anesthetic target relevant to oncologic recovery rather than a comfort-oriented endpoint alone. Emerging robotic platforms warrant evaluation against this expanded outcome set. FAU - Miyazaki, Takuro AU - Miyazaki T AUID- ORCID: 0000-0003-2718-2839 AD - Division of Thoracic and Pelvic Surgery, Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8501, Japan. FAU - Doi, Ryoichiro AU - Doi R AUID- ORCID: 0000-0003-3557-6387 AD - Division of Thoracic and Pelvic Surgery, Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8501, Japan. FAU - Matsumoto, Keitaro AU - Matsumoto K AD - Division of Thoracic and Pelvic Surgery, Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8501, Japan. LA - eng PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705381 OTO - NOTNLM OT - Return to Intended Oncologic Treatment OT - chronic postsurgical pain OT - intercostal nerve injury OT - lung cancer surgery OT - minimally invasive thoracic surgery OT - robot-assisted thoracic surgery OT - video-assisted thoracoscopic surgery COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:38 PHST- 2026/08/18 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - jcm15207843 [pii] AID - jcm-15-07843 [pii] AID - 10.3390/jcm15207843 [doi] PST - epublish SO - J Clin Med. 2026 Oct 12;15(20):7843. doi: 10.3390/jcm15207843. PMID- 42941356 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 11 TI - Liver Transplantation for Alcohol-Related Liver Disease in 2026: Phenotypes, Selection, and Post-Transplant Care. LID - 10.3390/jcm15207840 [doi] LID - 7840 AB - Liver transplantation (LT) for alcohol-related liver disease (ArLD) has evolved from a pathway centered on decompensated cirrhosis and prolonged abstinence to one that also encompasses severe alcohol-related hepatitis (sAH) and alcohol-related acute-on-chronic liver failure (ACLF). This change reflects the growing burden of ArLD and evidence that carefully selected patients with acute presentations can achieve excellent post-transplant survival. The traditional six-month abstinence rule has limited value as a stand-alone predictor of harmful post-transplant alcohol use. International guidance increasingly supports individualized multidisciplinary assessment of alcohol use disorder (AUD), psychiatric comorbidity, social vulnerability, insight, and treatment engagement, although listing requirements remain heterogeneous. Abstinence nevertheless remains a disease-modifying intervention that may promote clinical improvement, hepatic recompensation, and delisting in selected candidates. After LT, long-term care should integrate prevention and treatment of harmful alcohol use with surveillance and management of graft-related, cardiometabolic, oncologic, infectious, and other medical complications. This narrative Perspective critically appraises LT for ArLD in 2026 and proposes a phenotype-informed framework integrating medical urgency, dynamic reassessment, alcohol-use biomarkers, and longitudinal transplant-addiction care. FAU - Ferrarese, Alberto AU - Ferrarese A AUID- ORCID: 0000-0002-3248-2038 AD - Multivisceral Transplant Unit, Padua University Hospital, Via Giustiniani 2, 35122 Padua, Italy. AD - Department of Surgery, Oncology and Gastroenterology, Padua University, 35122 Padua, Italy. FAU - Perricone, Giovanni AU - Perricone G AUID- ORCID: 0000-0003-3890-5393 AD - Hepatology and Gastroenterology Unit, ASST Grande Ospedale Metropolitano Niguarda, 20162 Milan, Italy. FAU - Vivian, Laura Marta AU - Vivian LM AUID- ORCID: 0009-0007-4318-7206 AD - Department of Surgery, Oncology and Gastroenterology, Padua University, 35122 Padua, Italy. AD - Gastroenterology, Padua University Hospital, 35122 Padua, Italy. FAU - Zanetto, Alberto AU - Zanetto A AUID- ORCID: 0000-0002-6734-7178 AD - Department of Surgery, Oncology and Gastroenterology, Padua University, 35122 Padua, Italy. AD - Gastroenterology, Padua University Hospital, 35122 Padua, Italy. FAU - Viel, Guido AU - Viel G AUID- ORCID: 0000-0002-9769-535X AD - Legal Medicine and Toxicology, Department of Cardiac, Thoracic, Vascular Sciences and Public Health, Padua University, 35122 Padua, Italy. FAU - Burra, Patrizia AU - Burra P AUID- ORCID: 0000-0002-8791-191X AD - Department of Surgery, Oncology and Gastroenterology, Padua University, 35122 Padua, Italy. AD - Gastroenterology, Padua University Hospital, 35122 Padua, Italy. FAU - Louvet, Alexandre AU - Louvet A AUID- ORCID: 0000-0002-5293-007X AD - CHU Lille, Lille University, 59037 Lille, France. FAU - Germani, Giacomo AU - Germani G AD - Multivisceral Transplant Unit, Padua University Hospital, Via Giustiniani 2, 35122 Padua, Italy. AD - Department of Surgery, Oncology and Gastroenterology, Padua University, 35122 Padua, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261011 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705264 OTO - NOTNLM OT - acute-on-chronic liver failure OT - alcohol-related hepatitis OT - alcohol-related liver disease OT - liver transplantation OT - phosphatidylethanol OT - return to alcohol use COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/11 CRDT- 2026/10/27 10:38 PHST- 2026/07/30 00:00 [received] PHST- 2026/09/09 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - jcm15207840 [pii] AID - jcm-15-07840 [pii] AID - 10.3390/jcm15207840 [doi] PST - epublish SO - J Clin Med. 2026 Oct 11;15(20):7840. doi: 10.3390/jcm15207840. PMID- 42941351 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 11 TI - Diagnostic Accuracy of Artificial Intelligence Software in Intraoral, Panoramic, and CBCT Imaging: A Pilot Study. LID - 10.3390/jcm15207835 [doi] LID - 7835 AB - Background/Objectives: Artificial intelligence (AI) is increasingly used in dental radiology, but its performance may depend on the imaging modality and the analytical model. This pilot (feasibility) study evaluated the diagnostic accuracy of AI-based software (Diagnocat) in intraoral, panoramic, and cone-beam computed tomography (CBCT) images against a single-expert reference standard, accounting both for indications of pathology and for indications of its absence. Methods: In this retrospective analysis, 45 anonymized examinations (15 intraoral radiographs, 15 panoramic radiographs, and 15 CBCT) were assessed. The reference standard was established by an expert in dental and maxillofacial radiology with over 30 years of experience, who took no part in selecting the examinations and was blinded to the software output. The primary analysis was performed per diagnostic indication, defined as the combination of one examination, one diagnostic category, and one anatomical location. A secondary binary "pathology versus no pathology" analysis was performed with one observation per tooth or anatomical site. Because observations were clustered within examinations and patients, the analysis was descriptive; no hypothesis tests were performed, and no confidence intervals were reported. Results: In the primary per-indication analysis, 999 diagnostic indications were recorded in the reference standard and 1017 by the software; 791 were concordant, comprising 45 of 71 reference indications for intraoral radiographs, 358 of 452 for panoramic radiographs, and 388 of 476 for CBCT. In the secondary binary analysis, intraoral radiographs yielded sensitivity 0.79, specificity 0.59, precision 0.71, and F1-score (the harmonic mean of sensitivity and precision) 0.75; panoramic radiographs 0.73, 0.88, 0.79, and 0.75; and CBCT 0.80, 0.86, 0.78, and 0.79. Sensitivity and F1-score were numerically highest for CBCT. The three modality groups comprised different patients, anatomical regions, and distributions of diagnostic categories, so these differences cannot be attributed to the imaging modality alone. Conclusions: In this pilot study, the performance of the software varied across imaging modalities and diagnostic categories. Because the set of negative units was defined by the locations charted by the expert rather than by a complete anatomical inventory, specificity, negative predictive value (NPV), and accuracy are reported as secondary descriptive outcomes, are conditional on that operational definition, and are not compared between modalities. The findings are exploratory and hypothesis-generating. The software may support the interpretation of dental radiographs, but should not replace expert assessment. FAU - Czyz, Wojciech AU - Czyz W AUID- ORCID: 0009-0004-8084-0348 AD - Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 40-055 Katowice, Poland. FAU - Rozylo-Kalinowska, Ingrid AU - Rozylo-Kalinowska I AUID- ORCID: 0000-0001-5162-1382 AD - Department of Dental and Maxillofacial Radiodiagnostics, Faculty of Medical Dentistry, Medical University of Lublin, 20-093 Lublin, Poland. FAU - Dolibog, Pawel AU - Dolibog P AUID- ORCID: 0000-0003-4781-5162 AD - Department of Biophysics, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 40-055 Katowice, Poland. FAU - Skucha-Nowak, Malgorzata AU - Skucha-Nowak M AUID- ORCID: 0000-0003-0584-968X AD - Department of Dental Propedeutics, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 40-055 Katowice, Poland. LA - eng PT - Journal Article DEP - 20261011 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705470 OTO - NOTNLM OT - artificial intelligence OT - cone-beam computed tomography OT - dental radiology OT - diagnostic accuracy OT - diagnostic imaging OT - intraoral radiographs OT - panoramic radiograph COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/11 CRDT- 2026/10/27 10:38 PHST- 2026/08/19 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - jcm15207835 [pii] AID - jcm-15-07835 [pii] AID - 10.3390/jcm15207835 [doi] PST - epublish SO - J Clin Med. 2026 Oct 11;15(20):7835. doi: 10.3390/jcm15207835. PMID- 42941345 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 10 TI - Management of Renal Stones >2 cm in the Modern Endourology Era: Does PCNL Remain the Gold Standard? LID - 10.3390/jcm15207830 [doi] LID - 7830 AB - Background: Percutaneous nephrolithotomy (PCNL) remains the reference treatment for renal stones larger than 2 cm because of its high single-session stone-free rate. However, advances in flexible ureteroscopy (fURS), laser lithotripsy, suction-assisted access systems, and endoscopic technology have expanded the role of retrograde intrarenal surgery (RIRS) in patients who historically would have been treated primarily with PCNL. Objective: To critically review the contemporary roles of PCNL and RIRS in the management of renal stones >2 cm and evaluate whether advances in endoscopic and laser technologies have altered the traditional treatment paradigm. Methods: A focused narrative review of the contemporary literature was performed using PubMed, Scopus, and Web of Science. Priority was given to randomized trials, prospective comparative studies, multicenter registries, systematic reviews, meta-analyses, and international guideline recommendations evaluating efficacy, safety, retreatment, operative burden, and technological developments relevant to the management of large renal stones. Results: PCNL continues to provide the most reliable single-session stone clearance for large and complex renal calculi, particularly in patients with high stone burden and staghorn disease. RIRS offers lower procedural morbidity and faster recovery but may require staged procedures as stone burden increases. Recent developments, including thulium fiber laser, pulse-modulated Holmium:YAG systems, suction-assisted ureteral access sheaths, improved digital ureteroscopy, and intrarenal pressure-control strategies, have increased the efficiency and potential applicability of RIRS for larger stones. Nevertheless, current evidence does not support replacing PCNL as the reference treatment for complex or high-volume stone disease. Conclusion: PCNL remains the reference standard for renal stones >2 cm when maximal single-session clearance is the principal objective. Modern RIRS increasingly represents a clinically relevant alternative in appropriately selected patients. Rather than defining a single superior procedure, contemporary management should integrate stone burden and complexity, patient comorbidity, treatment goals, available technology, and local expertise. FAU - Samira, Nidal AU - Samira N AUID- ORCID: 0009-0005-8291-9209 AD - Urology Department, Barzilai Medical Center, Ben-Gurion University of the Negev, Ashkelon 8410501, Israel. FAU - Asali, Murad AU - Asali M AUID- ORCID: 0000-0003-2275-6805 AD - Urology Department, Barzilai Medical Center, Ben-Gurion University of the Negev, Ashkelon 8410501, Israel. LA - eng PT - Journal Article PT - Review DEP - 20261010 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705315 OTO - NOTNLM OT - intrarenal pressure OT - mini-PCNL OT - stone-free rate OT - suction ureteral access sheath COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:38 PHST- 2026/08/15 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - jcm15207830 [pii] AID - jcm-15-07830 [pii] AID - 10.3390/jcm15207830 [doi] PST - epublish SO - J Clin Med. 2026 Oct 10;15(20):7830. doi: 10.3390/jcm15207830. PMID- 42941342 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 10 TI - Prognostic Implication of a CT-FFR-Based Functional SYNTAX Score in Coronary Artery Disease Patients Undergoing Percutaneous Coronary Intervention. LID - 10.3390/jcm15207826 [doi] LID - 7826 AB - Background: This study aims to investigate the value of a SYNTAX score (SS) based on coronary computed tomographic angiography (cCTA)-derived fractional flow reserve (CT-FFR), termed "Functional SYNTAX score" (FSS(CTA)), to predict major adverse cardiovascular events (MACEs) in patients undergoing percutaneous coronary intervention (PCI). Methods: This cohort study included 876 patients who underwent cCTA and underwent PCI within 30 days during 2016-2020. CT-FFR was calculated by a machine learning algorithm. All 876 patients were divided into a low-risk group (22) with cCTA-based SS (SS(CTA)) as the control. Results: Based on the FSS(CTA), 10.4% (91/876) of patients were reclassified to the low-risk group, and 2.9% (25/876) of patients were reclassified to the intermediate-to-high-risk group. MACEs occurred in 16.6% (145/876) of patients over a median follow-up period of 19.4 months. FSS(CTA) and invasive coronary angiography-based SS (SS(ICA)) were independently associated with the risk of MACEs regardless of whether they were in continuous or categorical form (FSS(CTA): c continuous HR 1.082, 95% CI 1.038-1.128; categorical HR 1.865, 95% CI 1.138-2.305. SS(ICA): continuous HR 1.045, 95% CI 1.012-1.079; categorical HR 1.520, 95% CI 1.128-2.048). FSS(CTA) showed the best predictive power for MACEs compared with SS(CTA) and SS(ICA) (all comparisons with FSS(CTA)p < 0.05). Conclusions: FSS(CTA), SS(CTA) and SS(ICA) were all independent predictors of MACEs in patients undergoing PCI, and FSS(CTA) showed a significantly better predictive value for MACEs than SS(CTA) and SS(ICA). FAU - Shao, Qiaoyu AU - Shao Q AUID- ORCID: 0000-0001-9238-2656 AD - Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China. FAU - Schoepf, Uwe Joseph AU - Schoepf UJ AD - Division of Cardiovascular Imaging, Medical University of South Carolina, Charleston, SC 29425, USA. FAU - Ma, Xiaoteng AU - Ma X AUID- ORCID: 0000-0003-4806-6458 AD - Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China. FAU - Xu, Lei AU - Xu L AUID- ORCID: 0000-0002-8499-0448 AD - Department of Radiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China. FAU - Zhou, Yujie AU - Zhou Y AD - Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China. FAU - Gao, Fei AU - Gao F AD - Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China. FAU - Wang, Zhijian AU - Wang Z AD - Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, China. LA - eng GR - 82370449/National Natural Science Foundation of China/ GR - DFL20240601/Beijing Hospitals Authority's Ascent Plan/ GR - 2024YFC2418902/The National key research and development program/ GR - Z240021/Beijing Municipal Natural Science Foundation - Key Research Program/ GR - 2024-2-2064/Capital Health Research and Development Special Fund/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13704955 OTO - NOTNLM OT - functional SYNTAX score OT - percutaneous coronary intervention OT - risk stratification COIS- Dr. Schoepf receives institutional research support and/or personal fees from Bayer, Bracco, Elucid Bioimaging, Guerbet, HeartFlow, Inc., Keya Medical, and Siemens. Keya Medical provided the DEEPVESSEL FFR software and assisted with CT-FFR analysis but had no role in study design, statistical analysis, data interpretation, or manuscript preparation. The investigators retained full access to all data and final analytical decisions. The other authors declare that they have no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:38 PHST- 2026/09/11 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - jcm15207826 [pii] AID - jcm-15-07826 [pii] AID - 10.3390/jcm15207826 [doi] PST - epublish SO - J Clin Med. 2026 Oct 10;15(20):7826. doi: 10.3390/jcm15207826. PMID- 42941341 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 10 TI - Admission Route and In-Hospital Mortality Among Trauma Patients Admitted to a Regional Trauma Center in South Korea: A Retrospective Cohort Study. LID - 10.3390/jcm15207822 [doi] LID - 7822 AB - Background: Studies comparing interhospital transfer with direct admission have reported inconsistent associations with mortality, and Korean evidence is limited. We evaluated admission-route associations with in-hospital mortality among patients admitted to a South Korean regional trauma center and explored mortality-associated factors after transfer. Methods: This retrospective receiving-center cohort included trauma admissions during 2022-2024. Patients arriving in cardiac arrest who died without return of spontaneous circulation were excluded. Logistic regression estimated adjusted associations; additional analyses varied adjustment sets and cohort restrictions, and transferred-subgroup analyses were exploratory. Results: Among 2464 patients, 1504 arrived directly and 960 were transferred; mortality was 18.6% vs. 12.0%. Transfer was associated with lower adjusted mortality odds (adjusted odds ratio [aOR], 0.54; 95% confidence interval [CI], 0.39-0.75) within this selected receiving-center cohort. Omitting arrival physiology and injury-to-center time yielded an aOR of 0.42 (95% CI, 0.32-0.55). Age and arrival Glasgow Coma Scale (GCS) scores were associated with mortality among transferred patients (aOR, 2.03 [95% CI, 1.68-2.46] for age 70 vs. 60 years and 0.75 [0.71-0.79] per 1-point GCS increase). Conclusions: In this receiving-center cohort, transfer was associated with lower in-hospital mortality. Among transferred patients, older age and lower arrival GCS scores were exploratory prognostic markers. These associations should not be interpreted as causal, because transferred patients had to survive until arrival, prehospital severity was unmeasured, and arrival measures may reflect prior care. FAU - Jung, Hagi AU - Jung H AD - Department of Emergency Medicine, Pusan National University Hospital, 179, Gudeok-ro, Seo-Gu, Busan 49241, Republic of Korea. FAU - Wang, Iljae AU - Wang I AUID- ORCID: 0000-0003-2479-2359 AD - Department of Emergency Medicine, Pusan National University Hospital, 179, Gudeok-ro, Seo-Gu, Busan 49241, Republic of Korea. FAU - Tae, Wonwoong AU - Tae W AD - Department of Emergency Medicine, Pusan National University Hospital, 179, Gudeok-ro, Seo-Gu, Busan 49241, Republic of Korea. FAU - Yeom, Seokran AU - Yeom S AD - Department of Emergency Medicine, Pusan National University Hospital, 179, Gudeok-ro, Seo-Gu, Busan 49241, Republic of Korea. FAU - Lee, Gibyung AU - Lee G AD - Department of Emergency Medicine, Pusan National University Hospital, 179, Gudeok-ro, Seo-Gu, Busan 49241, Republic of Korea. FAU - Park, Sungwook AU - Park S AUID- ORCID: 0000-0001-6699-8553 AD - Department of Emergency Medicine, Pusan National University Hospital, 179, Gudeok-ro, Seo-Gu, Busan 49241, Republic of Korea. LA - eng GR - Pusan National University Hospital/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705049 OTO - NOTNLM OT - emergency medical services OT - interhospital transfer OT - mortality OT - trauma OT - trauma center OT - trauma system COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:38 PHST- 2026/08/17 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - jcm15207822 [pii] AID - jcm-15-07822 [pii] AID - 10.3390/jcm15207822 [doi] PST - epublish SO - J Clin Med. 2026 Oct 10;15(20):7822. doi: 10.3390/jcm15207822. PMID- 42941337 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 10 TI - Dermoscopic Patterns in Granulomatous Diseases: A Narrative Review. LID - 10.3390/jcm15207821 [doi] LID - 7821 AB - Dermoscopy has gained increasing recognition in recent years as a valuable non-invasive supplementary tool in diagnosing various skin disorders. However, a comprehensive overview of the distinguishing features of various cutaneous granulomatous diseases remains limited. This narrative aims to summarize the overlapping dermoscopic patterns reported across various diseases such as leprosy, cutaneous tuberculosis, Mycobacterium marinum, granuloma annulare, necrobiosis lipoidica, sarcoidosis, granulomatous variant of rosacea, annular elastolytic giant cell granuloma, and lupus miliaris disseminatus faciei. Across these conditions, several overlapping dermoscopic features were observed including, yellow-orange structureless areas, white structureless areas, linear, dotted and branching vascular patterns. These shared patterns can limit early recognition and diagnostic specificity. When interpreted alongside clinical features, dermoscopy may help narrow the differential diagnosis. However, dermoscopy alone is inadequate for definitive diagnosis; histopathology remains the gold standard. This narrative highlights the overlapping dermoscopic features of various cutaneous granulomatous diseases, emphasizing its role as a complementary tool. FAU - Sunil, Sanjana Sara AU - Sunil SS AD - Center for Medical Education in English, Poznan University of Medical Sciences, 60-512 Poznan, Poland. FAU - Korecka, Katarzyna AU - Korecka K AUID- ORCID: 0000-0002-9473-1239 AD - Department of Dermatology and Venereology, Poznan University of Medical Sciences, 60-355 Poznan, Poland. FAU - Polanska, Adriana AU - Polanska A AD - Department of Dermatology and Venereology, Poznan University of Medical Sciences, 60-355 Poznan, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261010 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705128 OTO - NOTNLM OT - dermoscopy OT - granulomatous disorders OT - non-invasive imaging COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:38 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - jcm15207821 [pii] AID - jcm-15-07821 [pii] AID - 10.3390/jcm15207821 [doi] PST - epublish SO - J Clin Med. 2026 Oct 10;15(20):7821. doi: 10.3390/jcm15207821. PMID- 42941335 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 10 TI - Long-Term Outcomes According to the Initially Intended Stenting Strategy for Unprotected Distal Left Main Bifurcation PCI: A Single-Centre Retrospective Cohort Study. LID - 10.3390/jcm15207817 [doi] LID - 7817 AB - Background: Optimal stenting for distal unprotected left main coronary artery (LMCA) bifurcation disease remains uncertain. We evaluated long-term outcomes by initially intended strategy. Methods: This retrospective, single-centre cohort included 234 consecutive patients undergoing percutaneous coronary intervention (PCI) without acute coronary syndrome, classified by intended strategy irrespective of crossover. Cox models for all-cause mortality were adjusted for strategy, age, left ventricular ejection fraction, SYNTAX score, and diabetes, with anatomical and propensity-score sensitivity analyses. Secondary and subgroup analyses were exploratory. Results: Provisional and upfront two-stent strategies were intended in 145 and 89 patients. Two-stent patients had greater anatomical complexity. During median 49-month follow-up, mortality did not differ significantly (two-stent versus provisional: 16.9% versus 21.4%; p = 0.499; adjusted hazard ratio, 0.83; 95% confidence interval, 0.43-1.60; p = 0.579), with consistent sensitivity analyses. Older age, lower ejection fraction, and higher SYNTAX score were associated with mortality. Target-lesion revascularisation (7.8% versus 2.1%; p = 0.033) and in-hospital stroke (5.6% versus 0.7%; p = 0.031) were more frequent with intended two-stent PCI; other secondary outcomes did not differ significantly. Exploratory unadjusted comparisons showed lower target-lesion and target-vessel revascularisation with double-kissing crush (n = 74) versus conventional culotte (n = 15), limited by small samples and selection bias. Intravascular ultrasound was associated with lower mortality in exploratory adjusted analyses. Mortality was numerically lower with elective intra-aortic balloon pump support at ejection fraction /=50 years with suspected cognitive impairment in three family medicine practices in Iasi. One unblinded clinician administered all instruments and assigned the strata by integrated clinical assessment of functional status, MMSE performance, and Reisberg GDS staging, with approximate reference bands for both scales. Scores and their positions relative to these bands were summarised descriptively. Results: The strata comprised 53 participants with mild, 247 with moderate, and 26 with severe cognitive impairment; 39.3% of participants were illiterate or had /=2 years was evaluated in the subset of patients with vocal fold paralysis with available long-term data. Results: In vocal fold paralysis, AVQI, MPT, and VHI-10 improved significantly at all follow-up time points through 1 year. In vocal fold atrophy/sulcus, AVQI and VHI-10 showed significant improvement mainly at 1 and 3 months, whereas MPT did not show consistent improvement. The time-by-disease-group interaction was significant for AVQI (p = 0.006), but not for MPT (p = 0.239) or VHI-10 (p = 0.559). AVQI responder trajectories also differed between disease groups (p = 0.023). In the available long-term subset (n = 19 at each time point), all three outcomes remained significantly improved at 1.5 years and >/=2 years. At >/=2 years, responder rates were 41.9% for AVQI and 48.4% for VHI-10, and MPT functional recovery was 31.6%. Conclusions: Atelocollagen injection laryngoplasty was associated with sustained voice improvement through 1 year in vocal fold paralysis, with improvement also observed at >/=2 years in a selected subset. Effects in vocal fold atrophy/sulcus were less consistently sustained. FAU - Hosokawa, Kiyohito AU - Hosokawa K AUID- ORCID: 0000-0002-5169-1038 AD - Department of Otorhinolaryngology-Head and Neck Surgery, Graduate School of Medicine, The University of Osaka, Suita 565-0871, Osaka, Japan. AD - Department of Otorhinolaryngology, Japan Community Health Care Organization Osaka Hospital, Osaka 553-0003, Japan. AD - Department of Otorhinolaryngology-Head and Neck Surgery, Osaka International Medical & Science Center, Osaka 543-0042, Japan. FAU - Nozawa, Masayuki AU - Nozawa M AD - Department of Otorhinolaryngology-Head and Neck Surgery, Graduate School of Medicine, The University of Osaka, Suita 565-0871, Osaka, Japan. FAU - Kawabe, Takanari AU - Kawabe T AD - Department of Otorhinolaryngology-Head and Neck Surgery, Graduate School of Medicine, The University of Osaka, Suita 565-0871, Osaka, Japan. FAU - Nagata, Akihiro AU - Nagata A AD - Department of Otorhinolaryngology-Head and Neck Surgery, Graduate School of Medicine, The University of Osaka, Suita 565-0871, Osaka, Japan. FAU - Kitayama, Itsuki AU - Kitayama I AD - Department of Otorhinolaryngology-Head and Neck Surgery, Graduate School of Medicine, The University of Osaka, Suita 565-0871, Osaka, Japan. AD - Department of Otorhinolaryngology, Japan Community Health Care Organization Osaka Hospital, Osaka 553-0003, Japan. FAU - Aruga, Kenji AU - Aruga K AD - Department of Otorhinolaryngology-Head and Neck Surgery, Graduate School of Medicine, The University of Osaka, Suita 565-0871, Osaka, Japan. AD - Department of Otorhinolaryngology-Head and Neck Surgery, Osaka International Medical & Science Center, Osaka 543-0042, Japan. FAU - Yamashita, Maki AU - Yamashita M AD - Department of Otorhinolaryngology-Head and Neck Surgery, Graduate School of Medicine, The University of Osaka, Suita 565-0871, Osaka, Japan. AD - Department of Otorhinolaryngology, Japan Community Health Care Organization Osaka Hospital, Osaka 553-0003, Japan. FAU - Tsuda, Takeshi AU - Tsuda T AD - Department of Otorhinolaryngology-Head and Neck Surgery, Graduate School of Medicine, The University of Osaka, Suita 565-0871, Osaka, Japan. FAU - Ozono, Yoshiyuki AU - Ozono Y AUID- ORCID: 0000-0002-9769-121X AD - Department of Otorhinolaryngology-Head and Neck Surgery, Graduate School of Medicine, The University of Osaka, Suita 565-0871, Osaka, Japan. FAU - Tanaka, Hidenori AU - Tanaka H AD - Department of Otorhinolaryngology-Head and Neck Surgery, Graduate School of Medicine, The University of Osaka, Suita 565-0871, Osaka, Japan. FAU - Maeda, Yohei AU - Maeda Y AUID- ORCID: 0000-0003-1510-9191 AD - Department of Otorhinolaryngology-Head and Neck Surgery, Graduate School of Medicine, The University of Osaka, Suita 565-0871, Osaka, Japan. AD - Department of Otorhinolaryngology, Japan Community Health Care Organization Osaka Hospital, Osaka 553-0003, Japan. FAU - Fukusumi, Takahito AU - Fukusumi T AUID- ORCID: 0009-0002-1857-7548 AD - Department of Otorhinolaryngology-Head and Neck Surgery, Graduate School of Medicine, The University of Osaka, Suita 565-0871, Osaka, Japan. AD - Department of Otorhinolaryngology-Head and Neck Surgery, Osaka International Medical & Science Center, Osaka 543-0042, Japan. FAU - Ogawa, Makoto AU - Ogawa M AD - Department of Otorhinolaryngology-Head and Neck Surgery, Graduate School of Medicine, The University of Osaka, Suita 565-0871, Osaka, Japan. AD - Department of Otorhinolaryngology, Japan Community Health Care Organization Osaka Hospital, Osaka 553-0003, Japan. FAU - Inohara, Hidenori AU - Inohara H AD - Department of Otorhinolaryngology-Head and Neck Surgery, Graduate School of Medicine, The University of Osaka, Suita 565-0871, Osaka, Japan. LA - eng GR - JP25K12765/Japan Society for the Promotion of Science/ GR - JP26K20062/Japan Society for the Promotion of Science/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705511 OTO - NOTNLM OT - Acoustic Voice Quality Index OT - atelocollagen OT - glottic insufficiency OT - injection laryngoplasty OT - sulcus vocalis OT - vocal fold atrophy OT - vocal fold paralysis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:38 PHST- 2026/08/13 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - jcm15207772 [pii] AID - jcm-15-07772 [pii] AID - 10.3390/jcm15207772 [doi] PST - epublish SO - J Clin Med. 2026 Oct 9;15(20):7772. doi: 10.3390/jcm15207772. PMID- 42941288 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 9 TI - Correlates of the Lumbar-Femoral T-Score Difference in Older Adults: Sex Outweighs the Triglyceride-Glucose Index. LID - 10.3390/jcm15207773 [doi] LID - 7773 AB - Background/Objectives: We examined correlates of lumbar-femoral T-score differences (DeltaT), retaining the triglyceride-glucose (TyG) index as the prespecified primary exposure. Methods: This retrospective cross-sectional analysis of routine records included 528 geriatric outpatients assessed in Ankara, Turkiye, in 2023; primary regression used 506 complete cases. DeltaT was lumbar minus femoral-neck T-score. Device L1-L4 composites were used without vertebra-level exclusions because scan images were unavailable. Firth models and an exploratory post hoc arithmetic decomposition were secondary. Results: Male sex was associated with higher DeltaT (B = +0.984; p < 0.001), uniquely explaining 9.87% of variance versus 0.88% for TyG. TyG estimates (95% CI) were +0.243 (+0.033 to +0.453; p = 0.023) in the primary analysis, +0.214 (-0.010 to +0.438; p = 0.061) retaining two records excluded by plausibility limits, and +0.070 (-0.115 to +0.255; p = 0.458) excluding influential observations. The male lumbar-worse OR was 0.12 (95% CI 0.03-0.31), based on 3/165 male events. Among patients with femoral-neck osteoporosis, lumbar T-scores exceeded -2.5 in 22/30 men versus 43/89 women (post hoc, unadjusted). Conclusions: Sex explained more unique DeltaT variance than TyG. The TyG association was small and sensitive to analytic decisions. Unmeasured degeneration and selection bias limit interpretation. FAU - Erten, Ridvan AU - Erten R AUID- ORCID: 0000-0002-1939-4738 AD - Division of Geriatrics, Department of Internal Medicine, Faculty of Medicine, Kocaeli University, 41380 Kocaeli, Turkiye. FAU - Erdogan, Kubra AU - Erdogan K AUID- ORCID: 0000-0001-9193-5327 AD - Geriatrics Clinic, Ankara Bilkent City Hospital, 06800 Ankara, Turkiye. FAU - Poyrazer, Mehmet AU - Poyrazer M AUID- ORCID: 0000-0002-6390-2832 AD - Division of Endocrinology and Metabolism, University of Health Sciences Ankara Training and Research Hospital, 06230 Ankara, Turkiye. FAU - Cakir, Ozgur AU - Cakir O AUID- ORCID: 0000-0001-6565-9488 AD - Department of Radiology, Faculty of Medicine, Kocaeli University, 41380 Kocaeli, Turkiye. FAU - Dogrul, Rana Tuna AU - Dogrul RT AD - Geriatrics Clinic, Ankara Bilkent City Hospital, 06800 Ankara, Turkiye. FAU - Selvi Oztorun, Hande AU - Selvi Oztorun H AD - Division of Geriatrics, Department of Internal Medicine, Faculty of Medicine, Ankara Yildirim Beyazit University, 06800 Ankara, Turkiye. FAU - Silay, Kamile AU - Silay K AUID- ORCID: 0000-0003-0384-4957 AD - Division of Geriatrics, Department of Internal Medicine, Faculty of Medicine, Ankara Yildirim Beyazit University, 06800 Ankara, Turkiye. LA - eng GR - TAD-2026-5370/Kocaeli Universitesi/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705045 OTO - NOTNLM OT - T-score discordance OT - aged OT - bone mineral density OT - osteoporosis OT - sex characteristics OT - triglyceride-glucose index COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:38 PHST- 2026/08/17 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - jcm15207773 [pii] AID - jcm-15-07773 [pii] AID - 10.3390/jcm15207773 [doi] PST - epublish SO - J Clin Med. 2026 Oct 9;15(20):7773. doi: 10.3390/jcm15207773. PMID- 42941283 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 9 TI - Biological Variability and Biomedical Innovation in Personalized Orthodontics: A Systematic Review with Narrative Synthesis. LID - 10.3390/jcm15207767 [doi] LID - 7767 AB - Background/Objectives: Orthodontic treatment response varies considerably among patients because mechanical loading interacts with individual biological, anatomical, systemic, and behavioral characteristics. Advances in mechanobiology, molecular biomarkers, omics technologies, and artificial intelligence (AI) may support a more personalized approach to treatment. This systematic review (SR) aimed to evaluate human clinical evidence concerning these domains and their potential contribution to predicting orthodontic response, improving clinical decision-making, and reducing adverse effects. Methods: A systematic search of PubMed, Scopus, and Web of Science was conducted through June 2026. Primary studies involving human participants and addressing orthodontic tooth movement (OTM), biological variability, biomarkers, genomics, epigenetics, multi-omics, or AI-supported orthodontics were selected according to predefined eligibility criteria. The review was conducted and reported in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020, and heterogeneous evidence was synthesized narratively across four predefined domains. The review protocol was registered in the International Prospective Register of Systematic Reviews (PROSPERO; registration number CRD420261486348). Risk of bias was assessed using design-appropriate instruments. Certainty of evidence was assessed at the outcome level using GRADE principles adapted to narrative synthesis. Results: The search identified 1051 records, of which 14 primary human studies were included. The clinical evidence comprised randomized trials, longitudinal and cross-sectional biomarker studies, genetic association studies, and a case report. Molecular and multi-omics studies identified candidate markers of biological response and patient phenotype. The included computational studies examined genetic association rules, gingival-enlargement classification, and AI-assisted root-resorption measurement; none demonstrated AI-guided selection of orthodontic forces. However, small samples, methodological heterogeneity, limited adjustment for confounding, and lack of external validation restricted clinical translation. Certainty of evidence supporting clinical personalization applications was predominantly very low. Conclusions: Personalized orthodontics should remain grounded in comprehensive diagnosis, biologically compatible mechanics, and individualized monitoring. Molecular and computational tools may complement clinical assessment, but routine implementation requires standardized protocols, multicenter validation, and prospective evidence of patient-relevant benefit. FAU - La Rosa, Salvatore AU - La Rosa S AUID- ORCID: 0009-0001-6910-3118 AD - Department of General Surgery and Surgical-Medical Specialties, School of Dentistry, University of Catania, Via S. Sofia 78, 95124 Catania, Italy. FAU - Leonardi, Rosalia AU - Leonardi R AUID- ORCID: 0000-0002-9433-9528 AD - Department of General Surgery and Surgical-Medical Specialties, School of Dentistry, University of Catania, Via S. Sofia 78, 95124 Catania, Italy. FAU - D'Anto, Vincenzo AU - D'Anto V AUID- ORCID: 0000-0003-1695-0927 AD - Department of Neurosciences, Reproductive Sciences and Oral Sciences, School of Orthodontics, University of Naples Federico II, Via Pansini 5, 80131 Naples, Italy. FAU - Ragusa, Rosalia AU - Ragusa R AUID- ORCID: 0000-0002-0964-3438 AD - Unit of Internationalization, Innovation and Health Research, University Hospital "G. Rodolico-San Marco", Via Santa Sofia 78, 95123 Catania, Italy. FAU - Isola, Gaetano AU - Isola G AUID- ORCID: 0000-0003-4267-6992 AD - Department of General Surgery and Surgical-Medical Specialties, School of Dentistry, University of Catania, Via S. Sofia 78, 95124 Catania, Italy. FAU - Polizzi, Alessandro AU - Polizzi A AUID- ORCID: 0000-0001-6717-8899 AD - Department of General Surgery and Surgical-Medical Specialties, School of Dentistry, University of Catania, Via S. Sofia 78, 95124 Catania, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261009 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705211 OTO - NOTNLM OT - artificial intelligence OT - biomarkers OT - epigenetics OT - genomics OT - mechanobiology OT - multi-omics OT - orthodontic tooth movement OT - personalized orthodontics OT - precision medicine COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:38 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - jcm15207767 [pii] AID - jcm-15-07767 [pii] AID - 10.3390/jcm15207767 [doi] PST - epublish SO - J Clin Med. 2026 Oct 9;15(20):7767. doi: 10.3390/jcm15207767. PMID- 42941281 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2077-0383 (Print) IS - 2077-0383 (Electronic) IS - 2077-0383 (Linking) VI - 15 IP - 20 DP - 2026 Oct 9 TI - Comparative Effects of Myo-Inositol, Metformin, Berberine, and Combination Therapy on Metabolic, Anthropometric and Hormonal Parameters in Women with Polycystic Ovary Syndrome: A 6-Month Observational Cohort Study. LID - 10.3390/jcm15207765 [doi] LID - 7765 AB - Background: Polycystic ovary syndrome (PCOS) is the most prevalent endocrine condition among women of reproductive age. It is marked by metabolic and reproductive irregularities and is often linked to insulin resistance, dyslipidemia, and obesity. Diverse methods of treatment are employed to enhance metabolic and hormonal parameters, including inositol-based supplements, metformin, and novel compounds such as berberine. Objective: To describe changes observed over six months in anthropometric, metabolic, lipid, and hormonal metrics among women with PCOS undergoing myo-inositol, metformin, berberine, or a combination of metformin and berberine in standard clinical settings, and to investigate if disparities among treatment groups remained after controlling for initial characteristics. Materials and Methods: This retrospective observational cohort study included 148 women with PCOS diagnosed according to the Rotterdam criteria who completed six months of treatment with myo-inositol (n = 38), metformin (n = 52), berberine (n = 28), or combined metformin-berberine therapy (n = 30). Anthropometric, metabolic, lipid, and androgen parameters were assessed at baseline and after six months. Within-group changes were evaluated with correction for multiple testing using the Holm method. Given the non-random treatment allocation and baseline differences among groups, supplementary baseline-adjusted analyses were performed for the continuous outcomes. Results: Treatment groups differed significantly in several baseline metabolic variables including body mass index (BMI), fasting plasma glucose (FPG), fasting insulin and homeostatic model assessment of insulin resistance (HOMA-IR). After Holm correction for multiple testing, the metformin group showed significant reductions in BMI, FPG, markers of insulin resistance, lipid parameters, and androgen-related hormonal parameters, together with an increase in high-density lipoprotein cholesterol (HDL-C). In the myo-inositol group, significant reductions were observed in fasting insulin, HOMA-IR, total cholesterol, low-density lipoprotein cholesterol (LDL-C), triglycerides, androstenedione, and dehydroepiandrosterone sulfate (DHEAS). In the berberine group, significant reductions were observed in BMI, fasting insulin, HOMA-IR, total cholesterol, triglycerides, and androstenedione. In the combined metformin-berberine group, significant reductions were observed in BMI, fasting insulin, HOMA-IR, total cholesterol, LDL-C, triglycerides, testosterone, androstenedione, DHEAS, and 17-hydroxyprogesterone, together with an increase in HDL-C. In the baseline-adjusted analyses, no significant overall treatment-group effects remained for the principal metabolic outcomes after correction for multiple testing, whereas significant overall treatment-group effects were observed for DHEAS and 17-hydroxyprogesterone. Conclusions: In this real-world observational cohort, six months of treatment with metformin, myo-inositol, berberine, or combined metformin-berberine therapy was associated with changes in several metabolic and hormonal parameters. However, substantial baseline differences among the clinically selected treatment groups and the absence of significant baseline-adjusted between-group effects for the principal metabolic outcomes preclude conclusions regarding comparative treatment efficacy. The observed between-group differences in selected hormonal outcomes warrant further investigation in prospective comparative studies. FAU - Yanachkova, Vesselina AU - Yanachkova V AUID- ORCID: 0000-0002-4205-7502 AD - Research Institute, Medical University-Pleven, 5800 Pleven, Bulgaria. AD - Department of Endocrinology, Specialized Hospital for Active Treatment of Obstetrics and Gynecology "Dr. Shterev", 1330 Sofia, Bulgaria. FAU - Yordanov, Angel AU - Yordanov A AUID- ORCID: 0000-0002-7719-382X AD - Department of Gynecologic Oncology, Medical University-Pleven, 5800 Pleven, Bulgaria. FAU - Yaneva, Antonia AU - Yaneva A AUID- ORCID: 0000-0002-8778-2660 AD - Department of Medical Informatics, Biostatistics and E-learning, Faculty of Public Health, Medical University-Plovdiv, 4002 Plovdiv, Bulgaria. FAU - Lebanova, Hristina AU - Lebanova H AUID- ORCID: 0000-0003-4835-6965 AD - Department of Pharmaceutical Sciences and Social Pharmacy, Faculty of Pharmacy, Medical University-Pleven, 5800 Pleven, Bulgaria. FAU - Stoev, Svetoslav AU - Stoev S AUID- ORCID: 0000-0001-6165-5674 AD - Department of Pharmaceutical Sciences and Social Pharmacy, Faculty of Pharmacy, Medical University-Pleven, 5800 Pleven, Bulgaria. LA - eng PT - Journal Article DEP - 20261009 PL - Switzerland TA - J Clin Med JT - Journal of clinical medicine JID - 101606588 PMC - PMC13705287 OTO - NOTNLM OT - PCOS OT - berberine OT - hyperandrogenism OT - insulin resistance OT - metabolic outcomes OT - metformin OT - myo-inositol OT - polycystic ovary syndrome COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:38 PHST- 2026/08/13 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - jcm15207765 [pii] AID - jcm-15-07765 [pii] AID - 10.3390/jcm15207765 [doi] PST - epublish SO - J Clin Med. 2026 Oct 9;15(20):7765. doi: 10.3390/jcm15207765. PMID- 42941277 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Oct 21 TI - Shenqi Granules Ameliorate Cerebral Ischemia-Reperfusion Injury by Regulating Glucocerebrosidase and Uridine Metabolism in Microglia. LID - 10.3390/metabo16100802 [doi] LID - 802 AB - Objective: Shenqi Granules is a traditional Chinese medicine formula derived from the classic prescription Shenqi Formula (SQF), composed of Astragali Radix (Huangqi) and Codonopsis Radix (Dangshen). SQF shows neuroprotection in cerebral ischemia-reperfusion injury (CIRI), but the underlying mechanisms remain unclear. Methods: The rat model of middle cerebral artery occlusion (MCAO) was established using the suture method to evaluate the neuroprotective effect of SQF. Blood-absorbed components were identified via UHPLC-Q-TOF-MS/MS, and potential targets and pathways were predicted through network pharmacology. Non-targeted metabolomics of serum and feces was performed using UHPLC-Orbitrap MS, and core metabolic pathways were selected based on regulation magnitude (RM). Gut microbiota structure was analyzed by 16S rRNA sequencing, with associations between microbiota and metabolites assessed via the Mantel test and redundancy analysis (RDA). Weighted gene co-expression network analysis (WGCNA) was applied to screen core Hub genes for ischemic stroke (IS). Based on single-cell RNA data, a uridine transport/metabolism-associated transcriptional signature score (Uridine_Score) was constructed, and cell type-specific coupling between glucocerebrosidase (GBA) and this transcriptional signature was analyzed. Microglial temporal trajectory analysis was conducted using Monocle3. Molecular docking between core components and GBA was verified by AutoDock, and GBA mRNA expression was confirmed by RT-qPCR. Results: High-dose SQF reduced infarct volume and neuronal damage and improved behavior. A total of 28 absorbed components and 31 shared IS targets were identified. Metabolomics revealed nucleotide metabolism as a core pathway common to both serum and fecal samples. SQF restored gut-blood uridine balance (serum uridine 0.41x Sham --> 0.85x post-SQF), reduced fecal dTMP and hypoxanthine. 16S rRNA sequencing demonstrated that the overall correlation between microbial community structure and nucleotide metabolites was statistically significant (Mantel r = 0.307-0.321, p < 0.01). WGCNA highlighted GBA, MMP9, and PYGL. Single-cell analysis showed a microglia-specific uridine transport/metabolism-associated transcriptional signature post-stroke, with GBA most correlated with this signature (rho = 0.377). Pseudotime revealed microglial state transitions (Homeostatic --> Acute_Reactive --> DAM), with positive GBA-uridine transport/metabolism-associated transcriptional coupling in the Homeostatic and DAM states. Riboflavin showed the strongest GBA binding (-9.83 kcal/mol), and SQF upregulated GBA mRNA in the penumbra (p < 0.0001). Conclusions: SQF reprograms nucleotide metabolism via the gut-blood axis and enhances microglial GBA-uridine transport/metabolism-associated transcriptional coupling. FAU - Liu, Hai-Xin AU - Liu HX AD - College of Traditional Chinese Medicine and Food Engineering, Shanxi University of Chinese Medicine, Taiyuan 030619, China. FAU - Li, Yu-Chang AU - Li YC AUID- ORCID: 0009-0004-3102-1901 AD - Third Clinical College, Shanxi University of Chinese Medicine, Taiyuan 030619, China. FAU - Liu, Cai-Xia AU - Liu CX AD - College of Traditional Chinese Medicine and Food Engineering, Shanxi University of Chinese Medicine, Taiyuan 030619, China. FAU - Yang, Ming-Kuan AU - Yang MK AUID- ORCID: 0009-0002-0004-2617 AD - College of Traditional Chinese Medicine and Food Engineering, Shanxi University of Chinese Medicine, Taiyuan 030619, China. FAU - Zhou, Shu-Yuan AU - Zhou SY AD - College of Traditional Chinese Medicine and Food Engineering, Shanxi University of Chinese Medicine, Taiyuan 030619, China. FAU - Dong, Ai-Ai AU - Dong AA AD - Second Clinical College, Shanxi University of Chinese Medicine, Taiyuan 030619, China. FAU - Pei, Ke AU - Pei K AD - College of Traditional Chinese Medicine and Food Engineering, Shanxi University of Chinese Medicine, Taiyuan 030619, China. FAU - Wen, Shi-Yuan AU - Wen SY AUID- ORCID: 0000-0001-9589-5676 AD - College of Basic Medical Sciences, Shanxi Medical University, Taiyuan 030600, China. LA - eng GR - zyytd2024040/Shanxi Provincial Administration of Traditional Chinese Medicine/ GR - 202304051001044/Shanxi Science and Technology Department/ GR - 82405592/National Natural Science Foundation of China/ GR - 2024ZYY2A034/Shanxi Provincial Administration of Traditional Chinese Medicine/ GR - 2024ZYY2C113/Shanxi Provincial Administration of Traditional Chinese Medicine/ GR - 2025PY-CXY-004/Shanxi University of Traditional Chinese Medicine/ GR - 2024PY-JL-16-02/Shaanxi University of Chinese Medicine/ GR - 2024PY-NS-027/Shaanxi University of Chinese Medicine/ GR - 82608011/National Natural Science Foundation of China/ GR - 2025XK18/Xinglin Talents Program of Shanxi University of Chinese Medicine/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13706725 OTO - NOTNLM OT - GBA OT - Shenqi granule OT - ischemic stroke OT - microglia OT - nucleotide metabolism OT - uridine metabolism COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:38 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - metabo16100802 [pii] AID - metabolites-16-00802 [pii] AID - 10.3390/metabo16100802 [doi] PST - epublish SO - Metabolites. 2026 Oct 21;16(10):802. doi: 10.3390/metabo16100802. PMID- 42941273 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Oct 21 TI - An Interpretable Ensemble-Learning Framework for Decoding Multi-Component-Multi-Bioactivity Relationships in Salvia miltiorrhiza Based on (1)H NMR Metabolomics and Lifecycle-Oriented Bioassays. LID - 10.3390/metabo16100798 [doi] LID - 798 AB - Background: Linking metabolite composition to biological activities remains challenging in herbal medicines, where diverse metabolites may collectively contribute to pharmacological effects. Although metabolite profiling is fundamental to quality assessment, conventional quality evaluation often provides limited information on how variation in metabolite composition relates to biological activities. Objectives: This study aimed to develop a multi-component-multi-bioactivity framework integrating metabolite profiling and bioactivity assessment, enabling bidirectional mapping between metabolite composition and biological responses and providing a functional perspective for quality characterization of complex herbal medicines. Salvia miltiorrhiza Bunge (Danshen), a widely used medicinal plant with a chemically diverse metabolome and well-documented cardiovascular activities, was selected as a representative model system. Methods: Quantitative (1)H NMR spectroscopy was employed to profile 28 metabolites in Danshen, while zebrafish-based bioassays were used to assess three cardiovascular-related bioactivities: pro-angiogenic, anti-thrombotic, and vasodilatory activities. A lifecycle-oriented approach was introduced for bioassay development to improve the robustness and reproducibility of biological measurements. Multiple machine learning approaches were integrated within a two-level stacking ensemble framework to construct quantitative metabolite-bioactivity relationship models and capture nonlinear associations between metabolite composition and biological activities. Results: The stacked models consistently outperformed the individual learners across the three cardiovascular bioactivities, achieving R(2) values of 0.895-0.931 and RMSE values of 0.116-0.130. External validation using 10 independent Danshen samples further supported the predictive reliability of the stacked models. Based on model-derived metabolite contributions, a quantitative and interpretable metabolite-bioactivity network linking 28 metabolites with three cardiovascular-related activities was constructed, revealing both shared and activity-specific metabolic contributors. Conclusions: This study establishes an interpretable framework integrating NMR-based metabolite profiling, robust multi-endpoint bioassays, and ensemble learning to connect metabolite composition with functional bioactivity. The framework provides a complementary bioactivity-informed perspective for the quality characterization of medicinal plants and supports systems-level evaluation of complex herbal medicines. FAU - Li, Wenzhu AU - Li W AD - Center for Clinical Pharmacy, Cancer Center, Department of Pharmacy, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou 310014, China. AD - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. AD - State Key Laboratory of Component-Based Chinese Medicine, Innovation Center in Zhejiang University, Hangzhou 310058, China. FAU - Li, Shasha AU - Li S AD - Center for Clinical Pharmacy, Cancer Center, Department of Pharmacy, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou 310014, China. FAU - Yu, Hang AU - Yu H AD - Center for Clinical Pharmacy, Cancer Center, Department of Pharmacy, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou 310014, China. AD - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. FAU - Zou, Xiaozhou AU - Zou X AD - Center for Clinical Pharmacy, Cancer Center, Department of Pharmacy, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou 310014, China. FAU - Lu, Yichao AU - Lu Y AD - Center for Clinical Pharmacy, Cancer Center, Department of Pharmacy, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou 310014, China. AD - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. FAU - Chen, Zhiyan AU - Chen Z AD - Center for Clinical Pharmacy, Cancer Center, Department of Pharmacy, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou 310014, China. AD - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. FAU - Wang, Nan AU - Wang N AD - Center for Clinical Pharmacy, Cancer Center, Department of Pharmacy, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou 310014, China. AD - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. FAU - Shi, Jiana AU - Shi J AD - Center for Clinical Pharmacy, Cancer Center, Department of Pharmacy, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou 310014, China. FAU - Ji, Kaiyue AU - Ji K AD - Center for Clinical Pharmacy, Cancer Center, Department of Pharmacy, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou 310014, China. FAU - Huang, Ping AU - Huang P AD - Center for Clinical Pharmacy, Cancer Center, Department of Pharmacy, Zhejiang Provincial People's Hospital (Affiliated People's Hospital), Hangzhou Medical College, Hangzhou 310014, China. LA - eng GR - QN25H280047/Zhejiang Provincial Natural Science Foundation/ GR - 82505303/National Natural Science Foundation of China/ GR - 2025KY650/Medical Health Science and Technology Project of Zhejiang Province/ GR - 2026ZF24/Traditional Chinese Medicine Science and Technology Project of Zhejiang Province/ GR - LQN25H310003/Zhejiang Provincial Natural Science Foundation/ GR - KYQN2024005/Basic Scientific Research Funds of Department of Education of Zhejiang Province/ GR - 2025ZYY54/Zhejiang Pharmaceutical Association Hospital Pharmacy Special Research Funding/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13707060 OTO - NOTNLM OT - AQbD OT - NMR OT - Salvia miltiorrhiza (Danshen) OT - bioassays OT - herbal medicine OT - metabolomics COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:38 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - metabo16100798 [pii] AID - metabolites-16-00798 [pii] AID - 10.3390/metabo16100798 [doi] PST - epublish SO - Metabolites. 2026 Oct 21;16(10):798. doi: 10.3390/metabo16100798. PMID- 42941271 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Oct 21 TI - Investigation of Nutrition Knowledge Levels Among Fitness Center Members According to Their Metabolic Profiles. LID - 10.3390/metabo16100796 [doi] LID - 796 AB - Background: This research aimed to examine the associations between the total "Nutrition Knowledge Scale" score and metabolic profile indicators among fitness center members, and to explore whether selected nutrition knowledge items differed according to daily meal consumption. Methods: The research, conducted using a cross-sectional and relational screening model, included a total of 230 volunteers, consisting of 67 women and 163 men. The participants' HDL, LDL, fasting glucose, and triglyceride levels were obtained from biochemical analysis results via the e-Nabiz system, while their nutrition knowledge levels were assessed using the "Nutrition Knowledge Scale". Descriptive statistics, simple and multiple linear regression analyses, and one-way ANOVA were used for data analysis. Results: The total "Nutrition Knowledge Scale" score was significantly associated with LDL levels after adjustment for BMI and gender (beta = -0.136, p = 0.037), whereas no statistically significant associations were observed with HDL, fasting glucose, or triglyceride levels. In secondary exploratory analyses, selected nutrition knowledge items showed significant associations with LDL levels, while the vitamin B12-related item was positively but not significantly associated with triglyceride levels (beta = 0.099, p = 0.136). Additionally, responses to three selected nutrition knowledge items differed significantly according to daily meal consumption, with small effect sizes (eta(2) = 0.029-0.046). Conclusions: Overall nutrition knowledge was associated with LDL levels after adjustment for BMI and gender, but not with HDL, fasting glucose, or triglyceride levels. Secondary exploratory findings indicated associations between selected nutrition knowledge items, LDL levels, and daily meal consumption. Causal inference is limited by the cross-sectional design. FAU - Apaydin, Murat Bekleyis AU - Apaydin MB AUID- ORCID: 0000-0002-7780-5849 AD - Department of Coaching Education, Faculty of Sport Sciences, Nigde Omer Halisdemir University, Nigde 51240, Turkiye. FAU - Ergin, Ridvan AU - Ergin R AD - Department of Physical Education and Sports, Faculty of Sport Sciences, Recep Tayyip Erdogan University, Rize 53100, Turkiye. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13706895 OTO - NOTNLM OT - cardiometabolic health OT - fitness OT - metabolic profile indicators OT - nutrition knowledge level OT - physical activity COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:38 PHST- 2026/08/01 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - metabo16100796 [pii] AID - metabolites-16-00796 [pii] AID - 10.3390/metabo16100796 [doi] PST - epublish SO - Metabolites. 2026 Oct 21;16(10):796. doi: 10.3390/metabo16100796. PMID- 42941270 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Oct 19 TI - Nutritional Strategies for Selected MASLD Outcomes: Metabolic Remodeling, Liver-Aging Biomarkers, and Public Health Priorities. LID - 10.3390/metabo16100795 [doi] LID - 795 AB - Metabolic dysfunction-associated steatotic liver disease (MASLD) connects nutritional exposures with cardiometabolic risk and vulnerability during aging. Nutrition provides an actionable foundation for care, while metabolomics may clarify variation in dietary responses. This critical narrative review integrates nutritional trials, metabolic and aging studies, and clinical guidance from PubMed, Web of Science, Scopus, and Google Scholar through September 2026 using a documented narrative-search and selection process. Evidence is differentiated by population, original disease definition, design, tissue source, biomarker role, and outcome. Sustained weight reduction and selected dietary interventions, including Mediterranean-style and other high-quality dietary patterns, can improve selected hepatic or cardiometabolic outcomes, although comparative superiority is inconsistent. Lipid handling, nutrient sensing, mitochondrial function, and microbial metabolites provide mechanistic context. Metabolomic signatures characterize dietary exposure and metabolic adaptation, whereas direct evidence that nutritional treatment slows liver-specific biological aging remains absent: such evidence would require a defined MASLD population, a nutritional intervention, and a liver-tissue or independently validated liver-specific aging endpoint. Nutritional effects on aging measures are heterogeneous and largely derived from populations not selected for MASLD. The synthesis identifies public health priorities: reducing cumulative dietary risk, integrating nutritional care with fibrosis and cardiometabolic assessment, preserving muscle function, and expanding access to sustained support. A qualification framework distinguishes exposure, association, prognosis, response, and candidate surrogate roles while incorporating cost and equity. Accessible nutritional care can improve selected MASLD outcomes now; aging-informed precision nutrition remains investigational until molecular testing improves decisions and durable health benefits beyond established assessment. FAU - Cai, Hairong AU - Cai H AD - The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou 510120, China. FAU - Cai, Huiling AU - Cai H AD - The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou 510120, China. FAU - Cai, Tianliang AU - Cai T AD - The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou 510120, China. FAU - Zhao, Huiying AU - Zhao H AUID- ORCID: 0009-0003-5180-4017 AD - School of Exercise and Health, Shanghai University of Sport, Shanghai 200438, China. FAU - Wang, Qiyue AU - Wang Q AD - School of Competitive Sports, Beijing Sport University, Beijing 100084, China. FAU - Liu, Chang AU - Liu C AUID- ORCID: 0000-0002-7867-6807 AD - School of Sport Science, Beijing Sport University, Beijing 100084, China. FAU - Wu, Wei AU - Wu W AUID- ORCID: 0000-0003-0401-7363 AD - School of Athletic Performance, Shanghai University of Sport, Shanghai 200438, China. FAU - Cai, Xingui AU - Cai X AD - The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou 510120, China. LA - eng PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13706974 OTO - NOTNLM OT - biological aging OT - epigenetic clocks OT - health equity OT - metabolic dysfunction-associated steatotic liver disease OT - metabolic remodeling OT - metabolomics OT - precision nutrition OT - public health COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:38 PHST- 2026/09/19 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - metabo16100795 [pii] AID - metabolites-16-00795 [pii] AID - 10.3390/metabo16100795 [doi] PST - epublish SO - Metabolites. 2026 Oct 19;16(10):795. doi: 10.3390/metabo16100795. PMID- 42941269 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Oct 19 TI - Peptidomics and Computational Docking Prioritize Candidate Peptide-Protein Associations Relevant to Upper-Gastrointestinal Biology in a Fermented Soy-Derived Postbiotic. LID - 10.3390/metabo16100794 [doi] LID - 794 AB - Background/Objectives: Gastro-AD((R)) (GAD) is a fermented soy-derived postbiotic supported by clinical and preclinical evidence related to gastric comfort and mucosal injury. Although fermentation-derived metabolites have been characterized, the peptide fraction remains poorly defined. This study characterized GAD peptides and explored their potential biological contexts. Methods: Untargeted peptidomics was used to identify soy-derived peptides in GAD and map them to source proteins. A diversity-sampling panel of ten peptides was selected using sequence, physicochemical, and predicted structural features. These peptides were screened against a human protein-structure library by computational docking, followed by GO enrichment and context-based prioritization of candidate interactors. Results: A total of 219 peptides, originating predominantly from soybean storage proteins, were identified. The GAD peptide fraction contained extensive overlapping sequence families and truncation ladders, consistent with extensive proteolytic processing. Three literature-reported antioxidant peptide sequences were identified as exact contiguous regions within longer GAD peptides. Large-scale docking against the screened human protein-structure library combined with GO-enrichment recurrence analysis and an independent review of top-ranked docking-derived candidates identified 180 candidate protein targets spanning innate immune and inflammatory defense, protease activity and peptide processing, extracellular-matrix remodeling, tissue repair and wound healing, and apoptotic signaling and epithelial turnover. Repeated randomized-affinity controls yielded substantially fewer FDR-significant GO terms than the observed docking-derived rankings, supporting non-random functional structure in the docking-derived protein rankings without establishing the accuracy of individual peptide-protein interactions. Context-based prioritization identified 43 unique Tier 1 and Tier 2 candidates with direct or context-dependent extracellular accessibility. The prioritized biological contexts included mucosal immune regulation, extracellular-matrix remodeling, epithelial turnover, and tissue restitution. Conclusions: The GAD peptide fraction described herein comprises multiple overlapping peptide families whose prioritized biological contexts center on mucosal defense, remodeling, and tissue restitution. These findings support further investigation of peptides as potential contributors to the multi-component biological properties of GAD. FAU - Tremblay, Annie AU - Tremblay A AD - Lallemand Bio-Ingredients, 1620 Prefontaine, Montreal, QC H1W 2N9, Canada. FAU - Harms, Chase AU - Harms C AD - Zero State Inc., Phoenix, AZ 85050, USA. FAU - Nankar, Amit AU - Nankar A AD - Zero State Inc., Phoenix, AZ 85050, USA. FAU - Gupta, Ravi AU - Gupta R AUID- ORCID: 0009-0007-1047-3740 AD - Zero State Inc., Phoenix, AZ 85050, USA. FAU - Buko, Alex AU - Buko A AD - Human Metabolome Technologies, Boston, MA 02134, USA. FAU - Tompkins, Thomas A AU - Tompkins TA AUID- ORCID: 0000-0002-2990-2265 AD - Lallemand Bio-Ingredients, 1620 Prefontaine, Montreal, QC H1W 2N9, Canada. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13707153 OTO - NOTNLM OT - Gastro-AD OT - computational docking OT - gastroprotection OT - peptidomics OT - postbiotic OT - soy peptides COIS- . A.B. is employed by Human Metabolome Technologies, which performed the peptidomics analysis described in this study on behalf of Lallemand Bio-Ingredients. C.H., A.N., and R.G. are employed by Zero State Inc., which performed the docking analyses described in this study on behalf of Lallemand Bio-Ingredients. A.T. and T.A.T. are employed by Lallemand Bio-Ingredients, which studies, produces, and markets Gastro-AD for the dietary supplement industry. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:38 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - metabo16100794 [pii] AID - metabolites-16-00794 [pii] AID - 10.3390/metabo16100794 [doi] PST - epublish SO - Metabolites. 2026 Oct 19;16(10):794. doi: 10.3390/metabo16100794. PMID- 42941268 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Oct 19 TI - Differences in Serum Cytokine Profiles Between Vegetarians and Omnivores: Exploratory Assessment of Dietary Factors. LID - 10.3390/metabo16100793 [doi] LID - 793 AB - Background/Objectives: Evidence suggests that plant-based diets are associated with reduced risk of chronic inflammatory diseases and lower circulating C-reactive protein (CRP) levels. However, associations with other inflammatory biomarkers, including interleukins (ILs), tumor necrosis factors (TNFs), interferons (IFNs), and chemokines, remain limited and inconclusive. This study compared multiple serum cytokine and chemokine levels between healthy vegetarians and omnivores. Methods: 160 apparently healthy, normal-weight participants aged 25-50 years, without acute and chronic illnesses or allergies, were included: 80 omnivores and 80 vegetarians (67 vegans and 13 lacto-ovo vegetarians). Serum concentrations of 13 cytokines/chemokines were measured using flow cytometry with a LEGENDplex bead-based multiplex immunoassay kit. Anthropometry, standard clinical hematological and biochemistry parameters, CRP, and dietary intakes were also analyzed. Results: The groups did not differ by sex (50% women), age, smoking, physical activity, or anthropometrics. Vegetarians had lower levels of TNF-alpha, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IFN-gamma, and IFN-alpha2, as well as lower leukocyte and lymphocyte counts, with no difference in IL-1beta, IL-18, IL-33, IL-23, MCP-1, or CRP. The differences persisted after adjustment for potential confounders (although fewer remained significant after multiple-testing correction), and were principally present in both vegetarian subgroups, alongside substantial differences in multiple dietary factors. Regression analyses further confirmed the association of vegetarian dietary patterns with cytokine levels that differed. Conclusions: Vegetarian dietary patterns were associated with lower circulating concentrations of selected cytokines/chemokines in this cohort. However, the number of associations remaining after multiple-testing correction was limited; there was high multicollinearity between dietary factors, and causality could not be established. Further research is needed to elucidate causal relationships, specific dietary factors, underlying mechanisms, and clinical implications. FAU - Sarac, Ivana AU - Sarac I AUID- ORCID: 0000-0003-1439-9561 AD - Group for Nutrition and Metabolism, Centre of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia. FAU - Mojsilovic, Slavko AU - Mojsilovic S AUID- ORCID: 0000-0002-4399-6720 AD - Group for Hematology and Stem Cells, Institute for Medical Research, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia. FAU - Despotovic, Marta AU - Despotovic M AD - Group for Nutrition and Metabolism, Centre of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia. FAU - Ivanov, Milan AU - Ivanov M AUID- ORCID: 0000-0001-8683-5751 AD - Group for Cardiovascular Physiology, Institute for Medical Research, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia. FAU - Paunovic, Marija AU - Paunovic M AUID- ORCID: 0000-0001-5148-7523 AD - Group for Nutritional Biochemistry and Dietology, Centre of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia. FAU - Milesevic, Jelena AU - Milesevic J AUID- ORCID: 0000-0002-6995-158X AD - Group for Nutrition and Metabolism, Centre of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia. FAU - Takic, Marija AU - Takic M AUID- ORCID: 0000-0001-8449-165X AD - Group for Nutrition and Metabolism, Centre of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, National Institute of the Republic of Serbia, University of Belgrade, 11000 Belgrade, Serbia. LA - eng GR - 451-03-66/2024-03/200015, 451-03-136/2025-03/200015, and 451-03-33/2026-03/200015/Ministry of Science, Technological Development, and Innovation/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13707265 OTO - NOTNLM OT - chemokine OT - cytokine OT - diet OT - immunity OT - inflammation OT - nutrition OT - plant-based OT - vegan OT - vegetarian COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:38 PHST- 2026/08/05 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - metabo16100793 [pii] AID - metabolites-16-00793 [pii] AID - 10.3390/metabo16100793 [doi] PST - epublish SO - Metabolites. 2026 Oct 19;16(10):793. doi: 10.3390/metabo16100793. PMID- 42941266 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Oct 19 TI - Dietary Apilarnil Supplementation Modulates Erythrocyte Redox Enzyme Activities in Rats Subjected to Repeated Swimming Exercise. LID - 10.3390/metabo16100791 [doi] LID - 791 AB - BACKGROUND: Exercise-induced reactive oxygen species (ROS) participate in both oxidative stress and physiological adaptation. Erythrocytes rely on NADPH-generating and thiol-related antioxidant systems to maintain redox homeostasis during exercise. Apilarnil, a drone brood-derived bee product containing diverse bioactive compounds, may influence redox-related enzymatic responses; however, its effects on erythrocyte NADPH-dependent and thiol-related enzyme activities during repeated exercise remain unclear. METHODS: Forty adult male Wistar rats were randomly assigned to five groups (n = 8/group): control (CN), acute exhaustive swimming (AES), short-term repeated swimming with vehicle (ST), repeated swimming plus apilarnil 0.2 g.kg(-1).day(-1) (ST + AP0.2), and repeated swimming plus apilarnil 0.4 g.kg(-1).day(-1) (ST + AP0.4). Repeated-swimming groups swam for 30 min/day for 14 consecutive days. Apilarnil was administered orally throughout the intervention. On Day 15, the AES and repeated-swimming groups underwent a terminal exhaustive swimming challenge. Erythrocyte G6PD, 6PGD, GST, GR, and TrxR activities were determined spectrophotometrically. RESULTS: Acute exhaustive swimming alone did not significantly alter erythrocyte enzyme activities. In contrast, repeated swimming exposure followed by the terminal exhaustive challenge was associated with higher G6PD, 6PGD, GST, GR, and TrxR activities compared with sedentary controls. Compared with ST, the higher apilarnil dose was associated with lower GST activity, and both doses were associated with lower GR and TrxR activities. G6PD and 6PGD activities did not differ significantly between apilarnil-treated and ST groups. CONCLUSIONS: Within the context of short-term repeated swimming, apilarnil-supplemented rats showed lower activities of selected erythrocyte redox enzymes than vehicle-treated rats. Because direct oxidative stress markers, hemolysis-related outcomes, and upstream molecular pathways were not assessed, these findings should be interpreted as evidence of enzymatic modulation rather than definitive evidence of oxidative protection or enhanced antioxidant adaptation. FAU - Simsek, Halil AU - Simsek H AD - Vocational School of Health Services, Bingol University, 12000 Bingol, Turkiye. FAU - Cakar, Fatih AU - Cakar F AUID- ORCID: 0000-0002-7551-4087 AD - Vocational School of Health Services, Bingol University, 12000 Bingol, Turkiye. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13707299 OTO - NOTNLM OT - NADPH OT - apilarnil OT - erythrocyte OT - glucose-6-phosphate dehydrogenase OT - oxidative stress OT - swimming exercise COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:38 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - metabo16100791 [pii] AID - metabolites-16-00791 [pii] AID - 10.3390/metabo16100791 [doi] PST - epublish SO - Metabolites. 2026 Oct 19;16(10):791. doi: 10.3390/metabo16100791. PMID- 42941263 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Oct 16 TI - Lactate-Driven Biphasic Reprogramming of Macrophage Immune Responses. LID - 10.3390/metabo16100788 [doi] LID - 788 AB - Lactate is a metabolic substrate, an extracellular signaling molecule, and a precursor for protein lactylation, with diverse effects on macrophage immunity. Evidence from infection, tissue injury, efferocytosis, and cancer indicates that lactate-associated pathways can amplify inflammation, support resolution and tissue repair, or sustain immune suppression. These outcomes depend on the timing, cellular source and compartment, chemical formulation and pH, transport and receptor engagement, and the chemistry and targets of lactylation. In this narrative review, we critically synthesize the biochemical foundations of lactate metabolism and lactyl-group donation and examine the mechanisms underlying these distinct macrophage responses. We distinguish model-specific biphasic responses from a universal sequence of early inflammation followed by resolution, and separate reparative programs from impaired antimicrobial or antitumor immunity. We also evaluate evidence for histone and non-histone lactylation, emphasizing the limits of metabolic perturbations and chromatin associations for establishing site-specific causality. This framework identifies experimental variables that should be controlled and supports context-selective investigation of lactate-related therapeutic targets in inflammatory diseases and cancer. FAU - Guo, Qingshuang AU - Guo Q AD - School of Medicine, Northwest University, Xi'an 710069, China. AD - Department of Plastic and Reconstructive Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. FAU - Ren, Pan AU - Ren P AD - Department of Burns, Plastic and Wound Repair Surgery, the Second Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710004, China. FAU - He, Lanqi AU - He L AD - Department of Plastic and Reconstructive Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. FAU - Gao, Jiaying AU - Gao J AD - Department of Plastic and Reconstructive Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. FAU - Lu, Ruiqi AU - Lu R AD - School of Medicine, Northwest University, Xi'an 710069, China. FAU - Wang, Yitian AU - Wang Y AD - Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China. FAU - Zhang, Zhiheng AU - Zhang Z AUID- ORCID: 0009-0006-3514-2502 AD - Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China. FAU - Han, Xiaohui AU - Han X AD - School of Medicine, Northwest University, Xi'an 710069, China. FAU - Zhang, Xi AU - Zhang X AD - Department of Plastic and Reconstructive Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. FAU - Fang, Chao AU - Fang C AUID- ORCID: 0000-0003-1011-2113 AD - Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi'an 710032, China. LA - eng GR - YJ(YB)202508/Second Affiliated Hospital of Xi'an Jiaotong University/ GR - 81903671/National Natural Science Foundation of China/ GR - 2025JC-YBQN-1037/Natural Science Basic Research Program of Shaanxi Province/ GR - 2025JC-QYCX-072/Natural Science Basic Research Program of Shaanxi Province/ PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13706687 OTO - NOTNLM OT - immune regulation OT - immunometabolism OT - lactate OT - lactylation OT - macrophage plasticity COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:38 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - metabo16100788 [pii] AID - metabolites-16-00788 [pii] AID - 10.3390/metabo16100788 [doi] PST - epublish SO - Metabolites. 2026 Oct 16;16(10):788. doi: 10.3390/metabo16100788. PMID- 42941258 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Oct 16 TI - Integrated Serum and Spatial Metabolomics Reveals Compartmentalized Metabolic Reprogramming and Ferroptosis Susceptibility in Nasopharyngeal Carcinoma. LID - 10.3390/metabo16100783 [doi] LID - 783 AB - BACKGROUND: Nasopharyngeal carcinoma (NPC) is a malignancy highly prevalent in southern China. Metabolic reprogramming is a hallmark of cancer, yet the interplay between the systemic and local metabolic landscapes of NPC remains poorly characterized. This study aimed to identify serum metabolic biomarkers and characterize local tissue metabolic alterations through integrated metabolomics. METHODS: We conducted a dual-compartment metabolomic investigation. Untargeted serum metabolomics was performed on 80 NPC patients and 35 healthy controls. Air flow-assisted desorption electrospray ionization mass spectrometry imaging (AFADESI-MSI) was applied to nasopharyngeal tissues (3 NPC vs. 3 chronically inflamed tissues). Cross-platform integration was performed to identify overlapping metabolic signatures, followed by least absolute shrinkage and selection operator (LASSO) regression for feature selection and logistic regression for biomarker panel construction. RESULTS: Serum metabolomics revealed systemic perturbations involving amino acid and propanoate metabolism. Spatial metabolomics identified glycerophospholipid metabolism as the most significantly altered pathway in NPC tissues, with markedly reduced phosphatidylserine in tumor regions, and the ferroptosis pathway was significantly enriched. Cross-omics integration identified 8 overlapping metabolites, of which 5 were mapped to the biosynthesis of unsaturated fatty acids. Cysteine demonstrated excellent diagnostic performance (area under the receiver operating characteristic curve (AUC) = 0.903). A 5-metabolite combined model achieved an AUC of 0.904 (95% confidence interval (CI): 0.892-0.916). CONCLUSIONS: NPC exhibits compartmentalized metabolic reprogramming characterized by systemic amino acid and propanoate perturbations alongside local glycerophospholipid remodeling and ferroptosis susceptibility. The identified 5-metabolite serum panel shows considerable potential as a non-invasive diagnostic tool for NPC. FAU - Hong, Huiling AU - Hong H AD - Department of Radiation Oncology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, NHC Key Laboratory of Cancer Metabolism, Fuma Road, Fuzhou 350014, China. FAU - Zhang, Yusong AU - Zhang Y AD - Department of Electronic Science, National Institute for Data Science in Health and Medicine, Xiamen University, Xiamen 361005, China. FAU - Yan, Linhui AU - Yan L AD - Department of Radiation Oncology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, NHC Key Laboratory of Cancer Metabolism, Fuma Road, Fuzhou 350014, China. FAU - Xu, Ting AU - Xu T AD - Department of Radiation Oncology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, NHC Key Laboratory of Cancer Metabolism, Fuma Road, Fuzhou 350014, China. FAU - Xu, Yiying AU - Xu Y AD - Department of Radiation Oncology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, NHC Key Laboratory of Cancer Metabolism, Fuma Road, Fuzhou 350014, China. FAU - Fei, Zhaodong AU - Fei Z AUID- ORCID: 0000-0001-9340-9558 AD - Department of Radiation Oncology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, NHC Key Laboratory of Cancer Metabolism, Fuma Road, Fuzhou 350014, China. FAU - Dong, Jiyang AU - Dong J AUID- ORCID: 0000-0002-1064-6548 AD - Department of Electronic Science, National Institute for Data Science in Health and Medicine, Xiamen University, Xiamen 361005, China. FAU - Chen, Chuanben AU - Chen C AUID- ORCID: 0000-0002-5994-8897 AD - Department of Radiation Oncology, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, NHC Key Laboratory of Cancer Metabolism, Fuma Road, Fuzhou 350014, China. LA - eng GR - 2022YNG02/Fujian Provincial Cancer Hospital/ GR - F2427Y-QYPT02-01/Fujian Provincial Cancer Hospital/ GR - F2427Y-QYPT02-02/Fujian Provincial Cancer Hospital/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13706798 OTO - NOTNLM OT - biomarker OT - cysteine OT - ferroptosis OT - mass spectrometry imaging OT - metabolic reprogramming OT - metabolomics OT - nasopharyngeal carcinoma OT - spatial metabolomics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:38 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - metabo16100783 [pii] AID - metabolites-16-00783 [pii] AID - 10.3390/metabo16100783 [doi] PST - epublish SO - Metabolites. 2026 Oct 16;16(10):783. doi: 10.3390/metabo16100783. PMID- 42941242 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Oct 12 TI - Diets Containing Defatted Black Soldier Fly Larvae Meal Improve Intestinal Morphology and Antioxidant Capacity in Wenchang Chickens. LID - 10.3390/metabo16100767 [doi] LID - 767 AB - Background/Objectives: This study evaluated the effects of dietary defatted black soldier fly (Hermetia illucens) larvae meal (DBSFLM) as a partial replacement of traditional dietary protein sources on growth performance, carcass traits, meat quality, serum biochemical and antioxidant-related parameters, ileal morphology, and cecal microbiota in Wenchang chickens. Methods: Ninety healthy 55-day-old male chickens were randomly assigned to three dietary treatments with six replicates of five birds each: a basal diet without DBSFLM (H0), or diets containing 3% (H3) or 5% (H5) DBSFLM for 26 days. Results: Average daily feed intake, average daily gain, and feed conversion ratio did not differ significantly among treatments (p > 0.05). Abdominal fat percentage was higher in H5 than in H0 (p < 0.05), whereas other measured carcass traits were unaffected. No significant differences were observed in the measured meat quality parameters, including pH, drip loss, cooking yield, shear force, and meat color (p > 0.05). Dietary DBSFLM increased serum glutathione peroxidase activity and decreased malondialdehyde concentration, while serum creatinine concentration decreased in both DBSFLM groups (p < 0.05). DBSFLM also decreased ileal crypt depth and increased the villus height-to-crypt depth ratio (p < 0.05). Changes in the relative abundances of Firmicutes_A, Proteobacteria, Akkermansia, Butyricicoccaceae, and Agathobaculum were observed in cecal microbiota. Conclusions: Overall, partial replacement of traditional dietary protein sources with 3-5% DBSFLM was associated with changes in selected serum parameters, ileal morphology, and cecal microbial composition, without significant differences in growth performance or most measured meat quality traits. FAU - Yang, Qingchao AU - Yang Q AD - Animal Nutrition and Feed Laboratory, School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, China. FAU - Bao, Ruiying AU - Bao R AD - Animal Nutrition and Feed Laboratory, School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, China. FAU - Huang, Liangmin AU - Huang L AD - Animal Nutrition and Feed Laboratory, School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, China. FAU - Wang, Xingke AU - Wang X AD - Animal Nutrition and Feed Laboratory, School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, China. FAU - Zhang, Yang AU - Zhang Y AD - Animal Nutrition and Feed Laboratory, School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, China. FAU - Cai, Qingying AU - Cai Q AUID- ORCID: 0009-0007-4505-0617 AD - Animal Nutrition and Feed Laboratory, School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, China. FAU - Peng, Zeru AU - Peng Z AD - Animal Nutrition and Feed Laboratory, School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, China. FAU - Shi, Huiyu AU - Shi H AD - Animal Nutrition and Feed Laboratory, School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, China. FAU - Zhang, Haiwen AU - Zhang H AD - Animal Nutrition and Feed Laboratory, School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, China. FAU - Li, Xiaochun AU - Li X AD - Animal Nutrition and Feed Laboratory, School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, China. FAU - Wang, Xuemei AU - Wang X AUID- ORCID: 0000-0002-0913-6065 AD - Animal Nutrition and Feed Laboratory, School of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, China. LA - eng GR - HNARS-06-G04/Department of Agriculture and Rural Affairs of Hainan Province/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13706901 OTO - NOTNLM OT - Wenchang chickens OT - antioxidant capacity OT - cecal microbiota OT - defatted black soldier fly larvae meal OT - ileal morphology COIS- The authors declare that this research has no conflicts of interest with any financial or nonfinancial relationships at the time of manuscript submission. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:38 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - metabo16100767 [pii] AID - metabolites-16-00767 [pii] AID - 10.3390/metabo16100767 [doi] PST - epublish SO - Metabolites. 2026 Oct 12;16(10):767. doi: 10.3390/metabo16100767. PMID- 42941235 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Oct 9 TI - Cultivar-Dependent Metabolite Signatures and Effects on Lipid Metabolism of Teas Processed from Three Varieties of Toona sinensis. LID - 10.3390/metabo16100760 [doi] LID - 760 AB - Background: The edible and medicinal plant Toona sinensis holds promise for functional food development, yet how cultivar variation affects the metabolite profiles and lipid-modulating effects of its processed leaf teas remains poorly understood. Methods: We systematically prepared and compared teas from three dominant cultivars-Green Toon (GT), Red Toon (RT), and Black Toon (BT)-using a standardized black tea processing procedure. Untargeted metabolomics combined with chemometric analysis was used to characterize cultivar-dependent metabolite signatures. Quantitative analysis was performed for total flavonoids, total polyphenols, and free amino acids. The lipid-modulating effects were evaluated in high-fat-diet-fed mice after 12-week oral administration of RT or BT (equivalent to 2.67 mg/g body weight per day, corresponding to ~14 g tea/day for a 60 kg adult), with GT as the comparison group. Results: Untargeted metabolomics with chemometric analysis revealed distinct cultivar-dependent metabolite signatures. RT and BT contained significantly higher levels of flavonoids and phenolic acids than GT. Notably, BT was putatively rich in flavonoid sulfates and chlorogenic acid derivatives, whereas RT putatively accumulated acylated flavonoid glycosides and theogallin. Quantitative analysis confirmed that RT and BT had higher contents of total flavonoids, total polyphenols, and free amino acids than GT. In high-fat-diet-fed mice, 12-week oral administration of RT or BT markedly attenuated body weight gain, hepatic steatosis, and dyslipidemia (improving triglycerides, total cholesterol, and LDL cholesterol), while GT showed only mild beneficial effects. Correlation analysis further demonstrated that polyphenols and flavonoids, particularly quercetin derivatives and specific phenolic acids, were significantly correlated with improvements in lipid metabolism parameters. Conclusions: These results highlight that cultivar selection is strongly associated with the chemical composition and lipid-regulatory effects of T. sinensis leaf teas, and suggest RT and BT as superior cultivars for the development of functional teas targeting metabolic health; these findings provide actionable data for evidence-based cultivar selection in the food industry. FAU - Wu, Guohuo AU - Wu G AUID- ORCID: 0000-0001-8619-7657 AD - Engineering Technology Research Center of Anti-Aging Chinese Herbal Medicine of Anhui Province, School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China. FAU - Tang, Yan AU - Tang Y AD - Engineering Technology Research Center of Anti-Aging Chinese Herbal Medicine of Anhui Province, School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China. FAU - Xu, Shuolei AU - Xu S AD - Engineering Technology Research Center of Anti-Aging Chinese Herbal Medicine of Anhui Province, School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China. FAU - Du, Zhaofeng AU - Du Z AD - Engineering Technology Research Center of Anti-Aging Chinese Herbal Medicine of Anhui Province, School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China. FAU - Xu, Ruojue AU - Xu R AD - Engineering Technology Research Center of Anti-Aging Chinese Herbal Medicine of Anhui Province, School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China. FAU - Du, Boyu AU - Du B AD - Engineering Technology Research Center of Anti-Aging Chinese Herbal Medicine of Anhui Province, School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China. FAU - Lu, Guangshun AU - Lu G AD - Engineering Technology Research Center of Anti-Aging Chinese Herbal Medicine of Anhui Province, School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China. FAU - Wu, Xiaoyun AU - Wu X AD - Engineering Technology Research Center of Anti-Aging Chinese Herbal Medicine of Anhui Province, School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China. FAU - Sui, Juanjuan AU - Sui J AD - Engineering Technology Research Center of Anti-Aging Chinese Herbal Medicine of Anhui Province, School of Biology and Food Engineering, Fuyang Normal University, Fuyang 236037, China. LA - eng GR - 2024AH051472/Anhui Provincial Department of Education/ GR - 2023KYQD0022/Fuyang Normal University/ GR - rcxm202515/Key Young Talent Program of Fuyang Normal University/ GR - 2024AH051474/Anhui Provincial Department of Education/ GR - 202510371007; 202510371038/National College Students' Innovation and Entrepreneurship Training Program/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13707263 OTO - NOTNLM OT - Toona sinensis OT - cultivar OT - flavonoids OT - lipid metabolism OT - metabolomics OT - tea COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:38 PHST- 2026/07/29 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - metabo16100760 [pii] AID - metabolites-16-00760 [pii] AID - 10.3390/metabo16100760 [doi] PST - epublish SO - Metabolites. 2026 Oct 9;16(10):760. doi: 10.3390/metabo16100760. PMID- 42941229 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Oct 8 TI - Bioactive Compounds from Cucurbita spp. and Their Processing By-Products: A Critical Review. LID - 10.3390/metabo16100754 [doi] LID - 754 AB - Pumpkin (Cucurbita spp.) processing generates peels, seeds, seed coats, pomace, and press cakes containing carotenoids, phenolics, proteins, unsaturated lipids, tocopherols, phytosterols, polysaccharides, and dietary fiber. This critical narrative review integrates composition and analytical characterization across anatomical fractions with extraction technologies, processing effects, biological evidence, and application readiness. Conventional extraction, pressing, supercritical carbon dioxide, deep eutectic solvents, enzyme-assisted extraction, ultrasound, microwaves, pulsed electric fields, and high-pressure homogenization differ in selectivity, recovery, and scalability. The reviewed evidence does not establish the environmental superiority of green extraction technologies without full process-level assessment. Processing can improve extractability or simulated bioaccessibility but also cause degradation or losses; concentration following water removal does not establish true retention. Experimental studies support potential antioxidant, anti-inflammatory, glycemia-related, antimicrobial, and antiproliferative activities, but most evidence remains chemical, cellular, or animal-based. Human evidence is preparation-specific and most developed for lower urinary tract symptoms; controlled evidence for glycemic and other systemic outcomes remains limited or inconsistent. Near-term opportunities include food ingredients, oils, protein fractions, pigment delivery, and selected packaging materials. Translation requires authenticated materials, validated analytical fingerprints, standardized preparations, safety and stability testing, adequately powered clinical trials for health-related claims, and mass, energy, economic, and life-cycle assessments. FAU - Kolars, Bela AU - Kolars B AUID- ORCID: 0009-0001-4985-5750 AD - Department of General Medicine and Geriatrics, Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia. AD - Health Center "Novi Sad", 21000 Novi Sad, Serbia. FAU - Minakovic, Ivana AU - Minakovic I AUID- ORCID: 0000-0002-6784-8618 AD - Department of General Medicine and Geriatrics, Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia. AD - Health Center "Novi Sad", 21000 Novi Sad, Serbia. FAU - Bosanac, Milana AU - Bosanac M AUID- ORCID: 0000-0002-9560-7176 AD - Department of Histology and Embryology, Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia. FAU - Gvozdenovic, Nemanja AU - Gvozdenovic N AD - Department of Emergency Medicine, Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia. AD - Clinic for Orthopedic Surgery and Traumatology, University Clinical Center of Vojvodina, 21000 Novi Sad, Serbia. FAU - Milak, Jovanka AU - Milak J AD - Clinic of Nephrology and Clinical Immunology, University Clinical Center of Vojvodina, 21000 Novi Sad, Serbia. AD - Doctoral Academic Studies, Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia. FAU - Petrovic, Nemanja AU - Petrovic N AD - Doctoral Academic Studies, Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia. AD - Clinic of Cardiovascular Surgery, Institute for Cardiovascular Diseases of Vojvodina, 21208 Sremska Kamenica, Serbia. FAU - Mijatovic Jovin, Vesna AU - Mijatovic Jovin V AUID- ORCID: 0000-0002-3200-0676 AD - Department of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine, University of Novi Sad, 21000 Novi Sad, Serbia. LA - eng GR - 002348498 2026 09418 002 000 000 001 04 002/Provincial Secretariat for Higher Education and Scientific Research, Autonomous Province of Vojvodina/ PT - Journal Article PT - Review DEP - 20261008 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13707147 OTO - NOTNLM OT - Cucurbita OT - bioaccessibility OT - biological activity OT - circular bioeconomy OT - green extraction OT - phytochemical profiling OT - processing OT - pumpkin by-products COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:38 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - metabo16100754 [pii] AID - metabolites-16-00754 [pii] AID - 10.3390/metabo16100754 [doi] PST - epublish SO - Metabolites. 2026 Oct 8;16(10):754. doi: 10.3390/metabo16100754. PMID- 42941225 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Oct 6 TI - Differential Modulation of Serum Albumin and Butyrylcholinesterase Following Pemafibrate Treatment in MASLD: A Post Hoc Analysis of the PEMA-FL Trial. LID - 10.3390/metabo16100750 [doi] LID - 750 AB - Background: Pemafibrate, a selective peroxisome proliferator-activated receptor-alpha (PPARalpha) modulator, improves liver stiffness and biochemistry in metabolic dysfunction-associated steatotic liver disease (MASLD). The longitudinal responses of serum albumin and butyrylcholinesterase during pemafibrate treatment remain incompletely characterized. We performed an exploratory post hoc analysis to characterize the effects of pemafibrate on these biomarkers in patients with non-cirrhotic MASLD. Methods: In this exploratory post hoc analysis of the randomized PEMA-FL trial, 118 patients with non-cirrhotic MASLD received pemafibrate (0.4 mg/day) or placebo for 72 weeks. Longitudinal changes in serum albumin and butyrylcholinesterase were evaluated. Between-group differences were estimated using ANCOVA, with MMRM performed as a sensitivity analysis. Results: Serum albumin and butyrylcholinesterase remained largely unchanged in the placebo group. In contrast, treatment was associated with a sustained increase in serum albumin and a sustained decrease in serum butyrylcholinesterase from week 4 through week 72. At week 72, the adjusted between-group difference in serum albumin was 0.18 g/dL (95% confidence interval [CI], 0.11 to 0.24). The adjusted between-group difference in serum butyrylcholinesterase was -33.5 U/L (95% CI, -45.2 to -21.7). Findings from the MMRM sensitivity analysis were consistent with those of the primary ANCOVA analyses. Conclusions: In this exploratory post hoc analysis, pemafibrate treatment was associated with a sustained increase in serum albumin and a sustained decrease in butyrylcholinesterase activity in patients with non-cirrhotic MASLD. These biomarkers should be interpreted individually because the mechanisms and clinical significance of their differing responses remain uncertain. ClinicalTrials.gov: NCT03350165; registered on 20 November 2017. FAU - Tanigawa, Ryohei AU - Tanigawa R AUID- ORCID: 0009-0005-8804-2014 AD - Global Clinical Development Department, Kowa Company Ltd., 4-14, 3-Chome, Nihonbashi-Honcho Chuo-ku, Tokyo 103-8433, Japan. AD - Department of Hepatology, Division of Metabolism, Faculty of Medicine, Saga University, 1-1, 5-Chome, Nabeshima, Saga 849-8501, Japan. FAU - Loomba, Rohit AU - Loomba R AD - MASLD Research Center, Division of Gastroenterology and Hepatology, Department of Medicine, University of California San Diego, La Jolla, CA 92037, USA. FAU - Eguchi, Yuichiro AU - Eguchi Y AD - Loco Medical General Institute, 1-1178, Mikazukichokanada, Ogi 845-0032, Japan. FAU - Takahashi, Hirokazu AU - Takahashi H AUID- ORCID: 0000-0003-1900-4389 AD - Department of Hepatology, Division of Metabolism, Faculty of Medicine, Saga University, 1-1, 5-Chome, Nabeshima, Saga 849-8501, Japan. FAU - Suganami, Hideki AU - Suganami H AD - Clinical Data Science Department, Kowa Company Ltd., 4-14, 3-Chome, Nihonbashi-Honcho Chuo-ku, Tokyo 103-8433, Japan. FAU - Belous, Andrey AU - Belous A AUID- ORCID: 0009-0003-4273-0343 AD - Clinical Development, Kowa Pharmaceutical Development, Inc., 500 Boylston Street, 17th Floor, Boston, MA 02116, USA. FAU - Nakajima, Atsushi AU - Nakajima A AUID- ORCID: 0000-0002-6263-1436 AD - Department of Gastroenterology, International University of Health and Welfare Atami Hospital, 13-1 Higashikaigancho, Atami 413-0012, Japan. LA - eng SI - ClinicalTrials.gov/NCT03350165 PT - Journal Article DEP - 20261006 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13706844 OTO - NOTNLM OT - MASLD OT - PPARalpha OT - albumin OT - butyrylcholinesterase OT - liver-derived circulating biomarkers OT - pemafibrate COIS- Rohit Loomba serves as a consultant to Aardvark Therapeutics, Altimmune, Arrowhead Pharmaceuticals, AstraZeneca, Cascade Pharmaceuticals, Eli Lilly, Gilead, Glympse Bio, Inipharma, Intercept, Inventiva, Ionis, Janssen Inc., Lipidio, Madrigal, Neurobo, Novo Nordisk, Merck, Pfizer, Sagimet, 89bio, Takeda, Terns Pharmaceuticals, and Viking Therapeutics. RL has stock options in Sagimet Biosciences. In addition, his institution received research grants from Arrowhead Pharmaceuticals, AstraZeneca, Boehringer-Ingelheim, Bristol-Myers Squibb, Eli Lilly, Galectin Therapeutics, Gilead, Intercept, Hanmi, Intercept, Inventiva, Ionis, Janssen, Madrigal Pharmaceuticals, Merck, Novo Nordisk, Pfizer, Sonic Incytes, and Terns Pharmaceuticals. RL is also the co-founder of LipoNexus Inc. RL receives funding support from NIDDK (P30DK120515) and the John C Martin Foundation (RP124). Yuichiro Eguchi has nothing to disclose. Hirokazu Takahashi received grants from Abbvie and GSK and payments for speakers from Kowa Company Ltd., Taisho Pharma, and Novo Nordisk. Atsushi Nakajima received research funding and grants from Kowa Company, Ltd. Andrey Belous is an employee of Kowa Pharmaceutical Development, Inc. Ryohei Tanigawa and Hideki Suganami are employees of Kowa Company, Ltd. The paper reflects the views of the scientists and not the company. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/06 CRDT- 2026/10/27 10:38 PHST- 2026/09/07 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/06 00:00 [pmc-release] AID - metabo16100750 [pii] AID - metabolites-16-00750 [pii] AID - 10.3390/metabo16100750 [doi] PST - epublish SO - Metabolites. 2026 Oct 6;16(10):750. doi: 10.3390/metabo16100750. PMID- 42941220 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Oct 3 TI - Aerobic and Aerobic-Oriented Exercise for Anthropometric, Androgen-Related, and Reproductive Endocrine Outcomes in Polyendocrine Metabolic Ovarian Syndrome (Polycystic Ovary Syndrome): A Systematic Review and Meta-Analysis. LID - 10.3390/metabo16100745 [doi] LID - 745 AB - Background: Aerobic exercise is recommended within lifestyle management for polycystic ovary syndrome (PCOS), but its effects on anthropometric and endocrine outcomes remain inconsistent. Methods: Five databases were searched from inception through July 2026. Controlled trials of structured aerobic or aerobic-oriented exercise lasting at least four weeks were eligible when the comparator did not receive an exercise program expected to improve aerobic fitness. Between-group changes were expressed as Hedges' g and pooled using random-effects models with cluster-robust small-sample inference. Subgroup, univariable meta-regression, and dose-response analyses were exploratory. Results: Twenty-two reports representing 20 independent trials were included. The pooled standardized estimate marginally favored exercise for body weight (g = -0.09, 95% CI -0.13 to -0.05), although its magnitude was trivial and the 95% prediction interval crossed the null (-0.29 to 0.11). Exercise was associated with lower body mass index (g = -0.20, 95% CI -0.33 to -0.06), waist-to-hip ratio (g = -0.36, 95% CI -0.56 to -0.19), total testosterone (g = -0.43, 95% CI -0.67 to -0.20), and free testosterone (g = -0.60, 95% CI -0.99 to -0.21), and with higher sex hormone-binding globulin (SHBG; g = 0.24, 95% CI 0.04 to 0.44). No clear pooled effects were observed for waist circumference, body fat percentage, free androgen index, dehydroepiandrosterone sulfate, or anti-Mullerian hormone. Follicle-stimulating hormone and luteinizing hormone estimates lay at the statistical boundary and were heterogeneous; leave-one-study-out inference was also unstable for free testosterone and SHBG. Certainty was low or very low across outcomes. Conclusions: Aerobic or aerobic-oriented exercise showed favorable pooled differences in selected anthropometric and androgen-related markers, but low certainty and the sensitivity of some estimates preclude an endocrine-targeted dose or reproductive-benefit claim. FAU - Qiu, Lanxin AU - Qiu L AD - Department of Applied Psychology, School of Health Management, Guangzhou Medical University, Guangzhou 511436, China. AD - School of Physical Education and Training, Xi'an Physical Education University, No. 1 Dongcheng South Road, Huyi District, Xi'an 710399, China. FAU - Huang, Yunzhi Emma AU - Huang YE AUID- ORCID: 0000-0001-5967-2731 AD - Nutrilite Health Institute, Amway R&D Center, 720 Cailun Road, Shanghai 201203, China. FAU - Zhuang, Mingnan AU - Zhuang M AD - College of Sports Training, Tianjin University of Sport, Tianjin 301617, China. FAU - Kan, Juntao AU - Kan J AUID- ORCID: 0000-0001-7280-6394 AD - Nutrilite Health Institute, Amway R&D Center, 720 Cailun Road, Shanghai 201203, China. AD - College of Sports Training, Tianjin University of Sport, Tianjin 301617, China. FAU - Qiu, Zhengqi AU - Qiu Z AUID- ORCID: 0000-0003-0033-0512 AD - Department of Applied Psychology, School of Health Management, Guangzhou Medical University, Guangzhou 511436, China. AD - Nutrilite Health Institute, Amway R&D Center, 720 Cailun Road, Shanghai 201203, China. LA - eng GR - Q0301-23 5/Guangzhou Postdoctoral Research Start-up Fund/ PT - Journal Article PT - Review DEP - 20261003 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13706977 OTO - NOTNLM OT - aerobic exercise OT - androgen OT - meta-analysis OT - polycystic ovary syndrome OT - polyendocrine metabolic ovarian syndrome OT - sex hormone-binding globulin COIS- J.K. and Z.Q. are employees of the Nutrilite Health Institute, Amway R&D Center; this relationship is disclosed for transparency. The authors declare no other conflicts of interest. Amway R&D Center had no role beyond the contributions of J.K. and Z.Q. described in the Author Contributions statement. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/03 CRDT- 2026/10/27 10:38 PHST- 2026/08/20 00:00 [received] PHST- 2026/09/13 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - metabo16100745 [pii] AID - metabolites-16-00745 [pii] AID - 10.3390/metabo16100745 [doi] PST - epublish SO - Metabolites. 2026 Oct 3;16(10):745. doi: 10.3390/metabo16100745. PMID- 42941217 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Oct 1 TI - Deciphering Vascular Smooth Muscle Cell Phenotype Switching via Integrated Single-Cell Transcriptomics and Machine Learning: CX3CR1 and COL1A1 as Novel Diagnostic Biomarkers for Atherosclerosis. LID - 10.3390/metabo16100742 [doi] LID - 742 AB - Background: Atherosclerosis is a chronic inflammatory disease of the arterial wall in which immune-cell accumulation and vascular stromal remodeling jointly shape plaque progression and stability. Because bulk-tissue transcriptomes combine signals from multiple cell types, the cellular origin of candidate expression markers must be resolved before they can be interpreted as vascular smooth muscle cell (VSMC)-intrinsic features. Methods: We integrated the GSE309462 human carotid-plaque single-cell RNA-sequencing dataset with the bulk arterial-tissue datasets GSE100927 and GSE57691. Intersection-derived genes were prioritized using LASSO, random forest, and SVM-RFE. Discriminatory direction was standardized for receiver operating characteristic analysis, the five consensus genes were evaluated in the independent plaque-stage dataset GSE28829, and their cellular origins were audited across 99,027 cells from 12 donors. Results: Forty-nine genes were shared between the SMC-cluster-associated gene set and the bulk arterial-tissue differential-expression results. Five genes (MPEG1, CX3CR1, SERPINA3, LMCD1, and COL1A1) were selected by all three machine-learning procedures. In the training data, direction-standardized AUCs ranged from 0.859 to 0.922. In GSE28829, MPEG1, CX3CR1, and COL1A1 showed higher expression in advanced plaques and AUCs of 0.904, 0.827, and 0.928, respectively; LMCD1 was higher in early plaques (AUC 0.870), whereas SERPINA3 showed limited discrimination (AUC 0.442). Single-cell localization showed that MPEG1 and CX3CR1 were predominantly expressed in macrophages, whereas LMCD1 and COL1A1 were enriched in VSMCs and other vascular stromal cells. Conclusion: The expression signature captures compartmentally distinct processes, particularly myeloid-cell infiltration and extracellular-matrix remodeling. These genes should therefore be interpreted as plaque-associated tissue markers and mechanistic candidates rather than validated non-invasive diagnostic biomarkers or definitive VSMC-lineage markers. FAU - Wang, Zhiqiang AU - Wang Z AD - College of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Zhao, Zhenyu AU - Zhao Z AD - College of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Hu, Zhixi AU - Hu Z AUID- ORCID: 0000-0002-2316-8645 AD - College of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Ye, Jiahao AU - Ye J AD - College of Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China. LA - eng GR - 82574922/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261001 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13706743 OTO - NOTNLM OT - COL1A1 OT - CX3CR1 OT - atherosclerosis OT - extracellular matrix OT - machine learning OT - myeloid infiltration OT - single-cell transcriptomics OT - vascular smooth muscle cell COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:38 PHST- 2026/08/24 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - metabo16100742 [pii] AID - metabolites-16-00742 [pii] AID - 10.3390/metabo16100742 [doi] PST - epublish SO - Metabolites. 2026 Oct 1;16(10):742. doi: 10.3390/metabo16100742. PMID- 42941215 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Oct 1 TI - Quercetin Differentially Modulates MTT-Derived Cellular Metabolic Activity and Apoptosis-Related Gene Expression in A549 and BEAS-2B Cells. LID - 10.3390/metabo16100740 [doi] LID - 740 AB - BACKGROUND/OBJECTIVES: Quercetin is a dietary flavonoid with potential anticancer activity, but comparative effects in malignant and non-malignant lung-derived epithelial cells remain incompletely characterized. METHODS: A549 and BEAS-2B cells were exposed to quercetin (1-250 microM) for 24, 48, and 72 h, and MTT-derived cellular metabolic activity was measured. Mean normalized concentration-response curves from three independent biological experiments (n = 3) were fitted with four-parameter logistic models, and Greenhouse-Geisser-corrected cell line x concentration x time interactions were tested. Apoptosis-related gene expression was assessed by qRT-PCR after exposure to 45 microM quercetin for 48 h; 45 microM was not a discrete concentration in the MTT series and was close to the directly tested 40 microM condition. RESULTS: Apparent MTT-derived IC(50) values were 58.28 microM (95% CI 44.18-74.81) for A549 and 121.22 microM (89.50-164.66) for BEAS-2B at 48 h, and 2.73 microM (2.31-3.67) and 1.49 microM (1.27-1.77), respectively, at 72 h. Cell line x concentration interactions were significant at all exposure times, as was the cell line x concentration x time interaction; transcriptional profiles differed descriptively between cell lines. CONCLUSIONS: The fitted IC(50) ordering reversed between 48 and 72 h, so a stable cancer-selective response is not supported. MTT reflects metabolic activity rather than cell death, and transcript changes do not establish functional apoptosis. FAU - Ozkan Vardar, Deniz AU - Ozkan Vardar D AUID- ORCID: 0000-0003-0976-9556 AD - Pharmacy Services Program, Vocational School of Health Services, Lokman Hekim University, 06510 Ankara, Turkiye. FAU - Duran, Tugce AU - Duran T AD - Department of Medical Genetics, Faculty of Medicine, KTO Karatay University, 42020 Konya, Turkiye. FAU - Aydin, Merve AU - Aydin M AUID- ORCID: 0000-0002-1522-6083 AD - Department of Medical Microbiology, Faculty of Medicine, Erzincan Binali Yildirim University, 24100 Erzincan, Turkiye. LA - eng GR - 20H04001/Lokman Hekim Universitesi/ PT - Journal Article DEP - 20261001 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13707059 OTO - NOTNLM OT - A549 OT - BEAS-2B OT - apoptosis-related gene expression OT - cancer OT - cellular metabolic activity OT - natural compound OT - quercetin OT - secondary plant metabolite COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:38 PHST- 2026/09/08 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - metabo16100740 [pii] AID - metabolites-16-00740 [pii] AID - 10.3390/metabo16100740 [doi] PST - epublish SO - Metabolites. 2026 Oct 1;16(10):740. doi: 10.3390/metabo16100740. PMID- 42941214 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Oct 1 TI - Linking Flaxseed Intake to Lipid and Metabolic Health: Associations with Metabolic Dysfunction Indices and LDL-Subfractions in Slovak Menopausal Women. LID - 10.3390/metabo16100739 [doi] LID - 739 AB - Background/Objectives: The aim of the present study was to investigate the effects of flaxseed consumption on selected metabolic health indicators in menopausal women following an eight-week dietary intervention. Methods: The study cohort comprised 34 menopausal women aged 45-55 years who were voluntarily recruited from the general population. The intervention lasted for eight weeks, during which participants received 40 g of ground flaxseed daily. Biochemical blood parameters were measured using a BioLis 24i Premium automatic analyser. Body composition was assessed using an InBody 720 device. Lipoprotein subfraction analysis was performed using the LipoPrint((R)) LDL System. Metabolic indices were calculated according to established equations. Results: Overall, the present findings suggest that the most pronounced changes following flaxseed supplementation occurred in selected lipid parameters, particularly LDL-C, total cholesterol, VLDL, IDL-A, and IDL-B. In contrast, HDL-C, TG, glucose, liver enzymes after multiple-testing correction, anthropometric measurements, and the investigated surrogate indices of adiposity and metabolic dysfunction did not show statistically significant changes. The calculated metabolic indices (C-I, BRI, HSI, LAP, TyG-I, TyG-BMI, TyG-WC, TyG-WHtR) suggest preferential visceral fat deposition and an alteration in adipose tissue distribution. Conclusions: Flaxseed supplementation produced a heterogeneous lipid response, with decreased LDL-C but increased total cholesterol, VLDL, and IDL. No significant changes were observed in other lipid, glycemic, anthropometric, or calculated metabolic parameters after correction for multiple comparisons. These findings do not support a uniformly favorable cardiometabolic effect and warrant further investigation. FAU - Lenartova, Petra AU - Lenartova P AUID- ORCID: 0000-0003-2899-7191 AD - Institute of Nutrition and Genomics, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia. FAU - Gazarova, Martina AU - Gazarova M AUID- ORCID: 0000-0001-8275-7311 AD - Institute of Nutrition and Genomics, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia. FAU - Ponichtera, Piotr AU - Ponichtera P AD - Department of Agronomy, Modern Technologies and Informatics, International Academy of Applied Sciences in Lomza, Studencka 19, 18-402 Lomza, Poland. FAU - Razna, Katarina AU - Razna K AUID- ORCID: 0000-0003-2121-131X AD - Institute of Plant and Environmental Sciences, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia. FAU - Nozkova, Janka AU - Nozkova J AD - Institute of Plant and Environmental Sciences, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Trieda Andreja Hlinku 2, 949 76 Nitra, Slovakia. LA - eng PT - Journal Article DEP - 20261001 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13707360 OTO - NOTNLM OT - flaxseed OT - lipid profile OT - lipoprotein subfractions OT - menopausal women OT - metabolic indices COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:38 PHST- 2026/08/03 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - metabo16100739 [pii] AID - metabolites-16-00739 [pii] AID - 10.3390/metabo16100739 [doi] PST - epublish SO - Metabolites. 2026 Oct 1;16(10):739. doi: 10.3390/metabo16100739. PMID- 42941212 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Sep 30 TI - Lipid Metabolic Reprogramming in Thyroid Cancer: Systemic Biomarkers, Tumor Dependencies and Therapeutic Resistance. LID - 10.3390/metabo16100736 [doi] LID - 736 AB - Lipid metabolic reprogramming is increasingly implicated in thyroid cancer progression and treatment resistance alongside circulating lipid associations are often conflated with tumor lipid dependency. A clinically useful synthesis must distinguish systemic lipid markers from intratumoral lipid flux before integrating them into mechanistic or therapeutic models. This critical narrative review integrates evidence across three connected layers: circulating lipid markers; tumor-intrinsic uptake, oxidation, synthesis, desaturation, cholesterol and oxysterol pathways; and immune-spatial niches involving macrophage lipid handling, APOE-associated polarization and ferroptosis-related lipid peroxidation. In papillary thyroid carcinoma, multi-omics and mechanistic studies implicate LPL-FATP2-CPT1A, PC-AKT/mTOR-SREBP1c-FASN and METTL16/YTHDC2/SCD1 programs in membrane remodeling, invasion-related behavior and recurrence risk stratification. In aggressive and anaplastic thyroid cancer, cholesterol synthesis signaling, fatty acid oxidation adaptation and SREBF1/SCD1-linked ferroptosis defense may contribute to resistance to radioiodine, radiotherapy, chemotherapy and BRAF/MEK inhibition. The current evidence is strongest for mechanistic and biomarker hypotheses; thyroid-cancer-specific prospective trials of lipid-directed treatment are lacking. Lipid metabolism therefore provides a framework for molecular stratification and treatment sensitization, but it does not currently justify nonspecific lipid lowering or routine lipid-targeted therapy. Translation will require paired blood and tumor lipidomics, spatial profiling, functional perturbation and biomarker-defined prospective studies. FAU - Zhou, Li AU - Zhou L AD - Department of Surgical Oncology, Affiliated Hangzhou First People's Hospital, School of Medicine, Westlake University, No. 261 Huansha Road, Shangcheng District, Hangzhou 310006, China. FAU - Mi, Yiqing AU - Mi Y AD - The Fourth Clinical Medical College, Zhejiang Chinese Medical University, No. 548 Binwen Road, Binjiang District, Hangzhou 310053, China. FAU - Mao, Linlin AU - Mao L AD - Department of Surgical Oncology, Affiliated Hangzhou First People's Hospital, School of Medicine, Westlake University, No. 261 Huansha Road, Shangcheng District, Hangzhou 310006, China. FAU - Shi, Jingjing AU - Shi J AD - Department of Surgical Oncology, Affiliated Hangzhou First People's Hospital, School of Medicine, Westlake University, No. 261 Huansha Road, Shangcheng District, Hangzhou 310006, China. FAU - Zhang, Yu AU - Zhang Y AD - Department of Surgical Oncology, Affiliated Hangzhou First People's Hospital, School of Medicine, Westlake University, No. 261 Huansha Road, Shangcheng District, Hangzhou 310006, China. FAU - Pan, Gang AU - Pan G AD - Department of Surgical Oncology, Affiliated Hangzhou First People's Hospital, School of Medicine, Westlake University, No. 261 Huansha Road, Shangcheng District, Hangzhou 310006, China. FAU - Peng, You AU - Peng Y AD - Department of Surgical Oncology, Affiliated Hangzhou First People's Hospital, School of Medicine, Westlake University, No. 261 Huansha Road, Shangcheng District, Hangzhou 310006, China. LA - eng GR - LGF22H070008/Zhejiang Provincial Natural Science Foundation/ GR - No. 2025KY1085/Zhejiang Provincial Health Commission/ PT - Journal Article PT - Review DEP - 20260930 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13707171 OTO - NOTNLM OT - SCD1 OT - cholesterol OT - fatty acid metabolism OT - ferroptosis OT - immunometabolism OT - lipid metabolism OT - lipidomics OT - therapeutic resistance OT - thyroid cancer COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:38 PHST- 2026/08/06 00:00 [received] PHST- 2026/09/10 00:00 [revised] PHST- 2026/09/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - metabo16100736 [pii] AID - metabolites-16-00736 [pii] AID - 10.3390/metabo16100736 [doi] PST - epublish SO - Metabolites. 2026 Sep 30;16(10):736. doi: 10.3390/metabo16100736. PMID- 42941208 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Sep 29 TI - Hepato-Immune Crosstalk Underlies Antipsychotic-Associated Metabolic Dysregulation in Schizophrenia: Quercetin as a Therapeutic Candidate for High-Inflammatory Subtypes. LID - 10.3390/metabo16100733 [doi] LID - 733 AB - Background: Patients with schizophrenia (SCZ) receiving antipsychotic treatment frequently develop metabolic complications, yet the mechanisms underlying liver-immune crosstalk remain poorly characterized. Methods: This study recruited 340 antipsychotic-treated SCZ patients and 340 matched healthy controls, with a 2-year follow-up restricted to the patient cohort. Candidate genes with hepatic metabolic relevance were screened from the independent GSE21138 brain transcriptomic dataset using machine learning. Molecular docking was performed to predict binding interactions of quercetin as a candidate bioactive compound. Results: Long-term antipsychotic exposure was associated with elevated serum 9-hydroxyrisperidone (9-OH-RISP) and substantial changes in liver function and metabolic markers; drug metabolite concentrations correlated with hepatic indices. Transcriptomic analysis identified 317 differentially expressed genes, from which 26 genes with liver-metabolism-related functions were selected as candidates. Seven hub genes (ABHD6, ALDH4A1, ETNPPL, etc.) were further identified via Support Vector Machine and Random Forest algorithms, and these genes were enriched in metabolic pathways, ferroptosis and other key signaling cascades. Unsupervised clustering based on hub gene expression stratified patients into high-inflammation (C2) and low-inflammation (C1) subtypes. The C2 subtype displayed computationally estimated enrichment of immune cells including B cells and naive CD8(+) T cells. Molecular docking predicted strong binding affinity (binding energy /= 1. The response was characterized by induction of metallothioneins, zinc transporters, NRF2- and AhR-associated genes. Increased expression of selected sterol-transport and intracellular-handling genes was accompanied by coordinated downregulation of cholesterol- and lanosterol-biosynthesis pathways. Reciprocal regulation of SPHK1, SGPP1, and SGPL1 indicated altered transcriptional control of S1P production and turnover. Junctional, cytoskeletal, TGF-beta-associated, and epithelial cell-state genes were also regulated. Comparative analysis identified 29 higher-amplitude DEGs meeting the predefined Burdock threshold criteria but not the corresponding comparator thresholds, including an 11-gene subgroup not significantly regulated by either comparator treatment. Conclusions: Together, these findings define an early transcriptional response integrating metal homeostasis, cytoprotective signaling, sterol and sphingolipid metabolism, and epithelial remodeling, providing a mechanistic framework for future investigation of Burdock rootextract nutritional and dermatological research. FAU - Bender, Cecilia AU - Bender C AUID- ORCID: 0000-0001-9510-9823 AD - ABC-Bioresearch, 37126 Verona, Italy. FAU - Suarez, Cynthia Gisela AU - Suarez CG AUID- ORCID: 0000-0002-9568-6556 AD - Martin Bauer Nutraceuticals Srl, 10121 Torino, Italy. AD - Finzelberg GmbH &CoKG, 56266 Andernach, Germany. FAU - Habarta, Alejandra Mariel AU - Habarta AM AD - ABC-Bioresearch, 37126 Verona, Italy. FAU - Blainski, Andressa AU - Blainski A AUID- ORCID: 0000-0002-4935-5270 AD - Martin Bauer Nutraceuticals Srl, 10121 Torino, Italy. AD - Finzelberg GmbH &CoKG, 56266 Andernach, Germany. FAU - Ruggiero, Alessandra AU - Ruggiero A AD - ABC-Bioresearch, 37126 Verona, Italy. AD - Department of Neurosciences, Biomedicine and Movement Sciences, School of Medicine, University of Verona, 37134 Verona, Italy. FAU - Stornaiuolo, Mariano AU - Stornaiuolo M AUID- ORCID: 0000-0003-2200-5083 AD - Department of Pharmacy, University of Naples Federico II, 80131 Naples, Italy. LA - eng PT - Journal Article DEP - 20260924 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13707041 OTO - NOTNLM OT - AhR OT - Arctium lappa OT - Burdock extract OT - NRF2 OT - cholesterol homeostasis OT - metallothioneins OT - skin s lipid metabolism OT - sphingolipid metabolism OT - transcriptomics OT - zinc homeostasis COIS- The authors declare that the research was conducted in the absence of commercial or financial relationships that could be construed as potential conflicts of interest. The manuscript reflects the scientific views of the authors. The companies involved did not influence the interpretation of the results or decisions concerning whether or what to publish. For transparency, Alejandra Habarta is an external collaborator and is not employed by ABC-Bioresearch. Cecilia Bender and Alessandra Ruggiero are founders of ABC-Bioresearch but are not employed by the company. The cellular and molecular analyses reported in the manuscript were conducted under the ABC-Bioresearch affiliation. Cynthia G. Suarez-Rizzo and Andressa Blainski were employed by Finzelberg GmbH & Co. KG during the production and characterization of the extracts for research purposes. Cynthia G. Suarez-Rizzo and Andressa Blainski are associated with Martin Bauer Nutraceuticals Srl. The paper reflects the views of the scientists and not the company. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:38 PHST- 2026/08/27 00:00 [received] PHST- 2026/09/10 00:00 [revised] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - metabo16100707 [pii] AID - metabolites-16-00707 [pii] AID - 10.3390/metabo16100707 [doi] PST - epublish SO - Metabolites. 2026 Sep 24;16(10):707. doi: 10.3390/metabo16100707. PMID- 42941176 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Sep 22 TI - Sex-Specific Differences in Metabolic Syndrome Classification Using IDF Versus NCEP-ATP III Criteria: A Systematic Review and Head-to-Head Meta-Analysis. LID - 10.3390/metabo16100701 [doi] LID - 701 AB - Background/Objectives: Metabolic syndrome (MetS) classification varies between the International Diabetes Federation (IDF) and National Cholesterol Education Program Adult Treatment Panel III (NCEP-ATP III) definitions. We compared their same-sample classification yield by sex. Methods: PubMed/MEDLINE and Scopus were searched from database inception through 30 August 2026, with reference-list screening and citation tracking. Eligible observational studies assessed both definitions in the same adult sample; intervention-effect reports without an eligible observational comparison were outside the scope. One reviewer performed selection, extraction, and JBI-based appraisal. Random-effects models pooled sex-specific prevalence and prevalence ratios; revised ATP III was examined in sensitivity analysis. The review was not registered. Results: Seven studies (95,887 participants) were included; five (74,399 participants; 36,033 men and 38,366 women) contributed to the primary meta-analysis. The pooled IDF/NCEP-ATP III prevalence ratio was 0.95 (95% CI 0.65-1.37) in men and 1.15 (1.01-1.31) in women. Sensitivity estimates were 0.91 (0.70-1.19) and 1.08 (0.92-1.27), respectively. Heterogeneity was extreme (I(2) 92.3-99.9%). Three primary studies were conducted in Asian countries and represented 89.1% of primary-analysis participants. Conclusions: No clear pooled difference was observed in men; the modest female excess under IDF attenuated in sensitivity analysis. Five independent studies, extreme heterogeneity, and geographically concentrated samples limit robustness and generalizability. The findings provide a hypothesis-generating evidence summary, not proof of diagnostic equivalence, biological mechanisms, prognostic superiority, or treatment benefit. Definition-specific and sex-stratified reporting is warranted. No external funding was received. FAU - Minh Bui Thi, Phuong AU - Minh Bui Thi P AUID- ORCID: 0009-0008-6945-9975 AD - Department of Biochemistry, Thai Binh University of Medicine and Pharmacy, Tran Lam Ward, Hung Yen 06122, Vietnam. FAU - Nguyen, Tien Van AU - Nguyen TV AUID- ORCID: 0000-0001-9022-1170 AD - Department of Health Organization and Management, Thai Binh University of Medicine and Pharmacy, Tran Lam Ward, Hung Yen 06122, Vietnam. FAU - Minh Le, Dat AU - Minh Le D AUID- ORCID: 0000-0002-3240-2376 AD - Vietnam Public Health Association, Dong Da Ward, Hanoi 11527, Vietnam. LA - eng PT - Journal Article PT - Review DEP - 20260922 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13706850 OTO - NOTNLM OT - International Diabetes Federation OT - NCEP-ATP III OT - classification OT - meta-analysis OT - metabolic syndrome OT - prevalence OT - sex differences OT - systematic review COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:38 PHST- 2026/08/30 00:00 [received] PHST- 2026/09/12 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - metabo16100701 [pii] AID - metabolites-16-00701 [pii] AID - 10.3390/metabo16100701 [doi] PST - epublish SO - Metabolites. 2026 Sep 22;16(10):701. doi: 10.3390/metabo16100701. PMID- 42941174 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 16 IP - 10 DP - 2026 Sep 22 TI - Colon-Specific Gut Microbiome Remodelling Is Associated with Host Metabolism in Paracetamol-Induced Liver Injury in Rats. LID - 10.3390/metabo16100698 [doi] LID - 698 AB - Background/Objectives: The gut and liver are functionally coupled along the gut-liver axis, yet most studies treat the intestine as a single compartment and sample feces alone. We tested whether a plant-derived beetroot-tarragon microgreen beverage engages this axis in a segment-selective, colon-anchored manner in a rat model of paracetamol-induced liver injury with the intestinal dysbiosis characteristic of this model. Methods: In a rat model of paracetamol-induced liver injury with the intestinal dysbiosis characteristic of this model, three paracetamol-exposed groups-a paracetamol-only model (APAP) and two treatment arms receiving either the beetroot-tarragon microgreen beverage (APAP+MG) or a licorice-root decoction (APAP+LIC)-were compared against an intact control serving as a reference. The gut-liver axis was interrogated end-to-end by combining liver histology, serum (1)H-NMR metabolomics and vitamin profiling, and 16S rRNA gene sequencing across five intestinal sites (duodenum, jejunum, ileum, colon, and faeces). Group differences were resolved with consensus differential-abundance testing, and colonic microbiota were linked to circulating metabolites through paired microbiome-metabolome integration. Results: Paracetamol produced a reproducible sub-massive injury. Microgreens were associated with less histological injury, less weight loss, and a serum metabolome closest to the intact state among the treated arms, although no individual metabolite contrast survived FDR correction. Community separation localised to the colon as a genuine centroid shift (Aitchison PERMANOVA R(2) = 0.22; PERMDISP non-significant), whereas small-intestinal separation partly reflected dispersion. The microgreen arm showed higher abundance of SCFA-associated Lachnospiraceae and lower Escherichia-Shigella, with a broader colonic response than licorice (27 vs. 4 differentially abundant genera). The microbiome-metabolome association was directional but exploratory; no genus-metabolite pair survived FDR correction. Conclusions: The beverage was associated with attenuation of paracetamol-induced hepatic injury (assessed histologically only; serum ALT/AST were not measured) through a colon-localised, SCFA-oriented remodelling with a directional but non-significant shift of the serum metabolome toward the intact profile, extending the microbiome-modulating benefits of microgreens to an acute drug-injury setting and supporting their further development as functional beverages, subject to a dedicated safety evaluation. As a pilot study with modest sample sizes and non-time-matched group endpoints, these findings require confirmation in adequately powered, time-matched cohorts. FAU - Khassenbekova, Zhanagul AU - Khassenbekova Z AUID- ORCID: 0000-0002-1472-9412 AD - Innovative Center ArtScience, Astana 010000, Kazakhstan. FAU - Kozhakhmetova, Saniya AU - Kozhakhmetova S AD - Innovative Center ArtScience, Astana 010000, Kazakhstan. FAU - Tarzhanova, Dinar AU - Tarzhanova D AUID- ORCID: 0000-0002-3740-1874 AD - Department of General Pharmacology, Non-Profit Joint-Stock Company "Astana Medical University", Astana 010000, Kazakhstan. FAU - Zhantureyeva, Akmaral AU - Zhantureyeva A AD - Department of General Pharmacology, Non-Profit Joint-Stock Company "Astana Medical University", Astana 010000, Kazakhstan. FAU - Uyzbayeva, Indira AU - Uyzbayeva I AUID- ORCID: 0000-0003-4282-4799 AD - Department of General Pharmacology, Non-Profit Joint-Stock Company "Astana Medical University", Astana 010000, Kazakhstan. FAU - Shoman, Aruzhan AU - Shoman A AD - The AgroTech Research and Innovation Center, Astana IT University, Astana 010000, Kazakhstan. FAU - Tultabayeva, Tamara AU - Tultabayeva T AD - Institute of Engineering and Food Technology, Saken Seifullin Kazakh Agrotechnical Research University, Astana 010000, Kazakhstan. AD - Department of Food Science and Technology, University of California, Davis, CA 95616, USA. FAU - Konysbayeva, Damilya AU - Konysbayeva D AD - Institute of Agriculture and Forestry, Saken Seifullin Kazakh Agrotechnical Research University, Astana 010000, Kazakhstan. FAU - Makangali, Kadyrzhan AU - Makangali K AUID- ORCID: 0000-0003-4128-6482 AD - Engineering Center for Organic Agricultural Technology, Saken Seifullin Kazakh Agrotechnical Research University, Astana 010000, Kazakhstan. FAU - Sagandyk, Assem AU - Sagandyk A AUID- ORCID: 0000-0001-5480-933X AD - Institute of Agriculture and Forestry, Saken Seifullin Kazakh Agrotechnical Research University, Astana 010000, Kazakhstan. LA - eng GR - No. BR24992852/Committee of Science of the Ministry of Science and Higher Education of the Republic of Kazakhstan/ PT - Journal Article DEP - 20260922 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC13706715 OTO - NOTNLM OT - 16S rRNA OT - Artemisia dracunculus OT - Beta vulgaris OT - acetaminophen hepatotoxicity OT - gut microbiota OT - gut-liver axis OT - microgreens OT - paracetamol OT - serum metabolomics OT - short-chain fatty acids COIS- Zhanagul Khassenbekova and Saniya Kozhakhmetova are employees of Innovative Center ArtScience. The paper reflects the views of the scientists and not the company. The funders had no role in the design of the study; in the collection, analyses or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:38 PHST- 2026/08/18 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - metabo16100698 [pii] AID - metabolites-16-00698 [pii] AID - 10.3390/metabo16100698 [doi] PST - epublish SO - Metabolites. 2026 Sep 22;16(10):698. doi: 10.3390/metabo16100698. PMID- 42941155 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 20 TI - Experimental Characterization and Numerical Assessment of the LONGASF Asphalt-Supported Ballastless Railway Track System. LID - 10.3390/ma19204415 [doi] LID - 4415 AB - The LONGASF ballastless track system combines longitudinal WZA concrete sleepers, an asphalt supporting layer, and the ICOSTRUN-03 fastening system. Although selected elements of the concept and preliminary numerical models have been reported previously, this paper presents, for the first time, the experimental characterization of the fastening system and an integrated assessment of the track response under vertical, transverse, and longitudinal loading. Laboratory tests were conducted to determine vertical stiffness, longitudinal and transverse rail resistance, and performance under repeated loading, including dynamic rail and sleeper displacement measurements. The experimentally identified parameters were subsequently implemented in simplified dynamic models and three-dimensional finite element models. After repeated loading, changes in longitudinal rail resistance and vertical stiffness remained within the limits adopted in the experimental programme. The numerical analyses demonstrated a strong dependence of track response on foundation stiffness and model extent. In the extended three-dimensional model, the maximum calculated horizontal and vertical displacements were 2.439 and 2.434 mm, respectively, for the weakest foundation condition considered. The simplified dynamic model indicated that sleeper acceleration may exceed the adopted 1 g criterion at the highest analysed speeds. However, because the model did not include vehicle inertia or coupled wheel-rail interaction, 250 km/h should be regarded solely as a parametric calculation case rather than a validated operating speed. The results provide a preliminary numerical assessment of the LONGASF concept and identify asphalt temperature sensitivity, foundation stiffness, fastening-system optimization, and full-scale model validation as key areas for further research. FAU - Chudyba, Lukasz AU - Chudyba L AUID- ORCID: 0000-0003-4500-1237 AD - Faculty of Civil Engineering, Cracow University of Technology, 31-155 Krakow, Poland. FAU - Urbanek, Malgorzata AU - Urbanek M AUID- ORCID: 0000-0002-8455-9981 AD - Faculty of Civil Engineering, Cracow University of Technology, 31-155 Krakow, Poland. FAU - Kudla, Dariusz AU - Kudla D AD - Faculty of Civil Engineering, Cracow University of Technology, 31-155 Krakow, Poland. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707091 OTO - NOTNLM OT - asphalt supporting layer OT - ballastless track OT - experimental characterization OT - finite element analysis OT - foundation stiffness OT - longitudinal concrete sleeper OT - rail fastening system OT - track dynamics COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:38 PHST- 2026/09/05 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - ma19204415 [pii] AID - materials-19-04415 [pii] AID - 10.3390/ma19204415 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 20;19(20):4415. doi: 10.3390/ma19204415. PMID- 42941150 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 20 TI - Development of New Materials with Different Types of By-Products to Manufacture Construction Elements for Thermal Insulation. LID - 10.3390/ma19204409 [doi] LID - 4409 AB - This study evaluates mortars that incorporate mixed recycled aggregates, biomass bottom ash, chamotte, wood waste, and expanded clay as partial substitutes for cement and natural aggregates. Three series of mortars were investigated: with 25% cement replacement, partial sand replacement, and simultaneous replacement of cement and sand. Mechanical, physical, durability, and thermal properties were evaluated, along with their relationships through a correlation analysis. Cement substitution did not result in a drastic reduction in compressive strength and produced limited thermal improvements, whereas sand substitution led to substantially greater reductions in thermal conductivity, ranging from approximately 10.3% for MRA to 59.1% for wood waste compared to the control. Expanded clay reduced thermal conductivity by 24.7%, while BBA-L showed greater insulating potential than BBA-PG but resulted in a reduction in compressive strength. The combination of chamotte, limestone filler as a cement substitute, MRA, and expanded clay as an aggregate substitute provided the best balance between thermal and mechanical performance, achieving a compressive strength of approximately 39 MPa at 90 days and reducing thermal conductivity by nearly 22% compared to the reference mortar. The results demonstrate that thermal performance depends largely on density and porosity, although the optimal selection of materials requires simultaneously considering both mechanical and physical properties. FAU - Musa, Ali AU - Musa A AD - Construction Engineering Area, University of Cordoba, 14071 Cordoba, Spain. AD - Department of Civil Engineering, Tobruk University, Tobruk, Libya. FAU - Ahmed, Leena AU - Ahmed L AUID- ORCID: 0009-0004-6723-858X AD - Construction Engineering Area, University of Cordoba, 14071 Cordoba, Spain. FAU - Rosales, Manuel AU - Rosales M AUID- ORCID: 0000-0003-2638-7998 AD - Construction Engineering Area, University of Cordoba, 14071 Cordoba, Spain. AD - Area of Mechanics of Continuous Media and Theory of Structures, University of Cordoba, 14071 Cordoba, Spain. FAU - Agrela, Francisco AU - Agrela F AUID- ORCID: 0000-0002-2380-4226 AD - Construction Engineering Area, University of Cordoba, 14071 Cordoba, Spain. FAU - Rosales, Julia AU - Rosales J AUID- ORCID: 0000-0001-6882-1196 AD - Construction Engineering Area, University of Cordoba, 14071 Cordoba, Spain. AD - Area of Mechanics of Continuous Media and Theory of Structures, University of Cordoba, 14071 Cordoba, Spain. LA - eng GR - PID2022-141028OB-C21/Ministry of Science and Information/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707220 OTO - NOTNLM OT - biomass bottom ash OT - chamotte OT - mechanical behavior OT - mixed recycled aggregates OT - sustainable mortars OT - thermal conductivity OT - wood waste COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:38 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - ma19204409 [pii] AID - materials-19-04409 [pii] AID - 10.3390/ma19204409 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 20;19(20):4409. doi: 10.3390/ma19204409. PMID- 42941146 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 20 TI - Low-Energy Mechanochemical Dry Coating for Improving the Flowability of Sodium Naproxen. LID - 10.3390/ma19204405 [doi] LID - 4405 AB - Sodium naproxen is a widely used nonsteroidal anti-inflammatory drug (NSAID) known for its therapeutic efficacy but also for its poor flowability, which complicates manufacturing processes. This study aimed to improve the flow properties of sodium naproxen through a patented mechanochemical dry coating method using nanostructured silica (Aerosil((R)) R972) as a flow modifier. The process was conducted in a modified V-type tumbler mixer under selected conditions, including a 7:1 ball-to-powder mass ratio, a 10-min mixing time, and a 10 rpm rotational speed. Flowability was evaluated using Carr's indices, while mechanical properties were assessed via shear testing with a Jenike cell. Surface morphology was characterized by SEM. Results showed that the modified sodium naproxen samples demonstrated a substantial improvement in flow indices, a shift to an "easy-flowing" classification at higher consolidation stresses, and a marked reduction in cohesion compared to the unmodified material. SEM confirmed partial silica deposition on particle surfaces. These findings indicate that the applied method effectively improves the handling characteristics of sodium naproxen powders. The process is relatively simple, requires low energy, is potentially applicable at a larger scale, and shows potential for pharmaceutical powder processing requiring flow enhancement of cohesive active ingredients. FAU - Przywara, Mateusz AU - Przywara M AUID- ORCID: 0000-0001-7009-7811 AD - Department of Chemical and Process Engineering, Rzeszow University of Technology, al. Powstancow Warszawy 6, 35-029 Rzeszow, Poland. FAU - Les, Karolina Maria AU - Les KM AUID- ORCID: 0000-0002-1112-8160 AD - Department of Chemical and Process Engineering, Rzeszow University of Technology, al. Powstancow Warszawy 6, 35-029 Rzeszow, Poland. FAU - Chutkowski, Marcin AU - Chutkowski M AUID- ORCID: 0000-0001-8234-4963 AD - Department of Chemical and Process Engineering, Rzeszow University of Technology, al. Powstancow Warszawy 6, 35-029 Rzeszow, Poland. LA - eng GR - RID/SP/0032/2024/01/Ministry of Science and Higher Education/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13706810 OTO - NOTNLM OT - Aerosil(R) R972 OT - Jenike shear cell OT - V-type mixer OT - cohesion OT - dry coating OT - mechanochemical processing OT - pharmaceutical excipients OT - powder flowability OT - silica nanoparticles OT - sodium naproxen COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:38 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - ma19204405 [pii] AID - materials-19-04405 [pii] AID - 10.3390/ma19204405 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 20;19(20):4405. doi: 10.3390/ma19204405. PMID- 42941141 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 19 TI - Study on the Shear Mechanical Behaviour of Asphalt Aggregate Interface Under the Combined Effect of Temperature and Asphalt Film Thickness. LID - 10.3390/ma19204400 [doi] LID - 4400 AB - The interface between asphalt and aggregate acts as a key link in load transfer and damage development; its shear mechanical behaviour has a decisive influence on the fatigue cracking and durability of bituminous pavements. In this study, SBS-modified asphalt and limestone aggregate were selected. By precisely controlling the moulding load, asphalt film thicknesses ranging from 23 mum to 219 mum were achieved. Systematic shear tests were conducted within a temperature range of 10 degrees C to 40 degrees C to systematically investigate the evolution of interfacial shear strength, stress-displacement curve characteristics, shear fracture energy and failure modes. Two primary failure modes were identified: adhesive failure, defined as debonding at the asphalt-aggregate interface with minimal residual asphalt on the aggregate surface; and cohesive failure, defined as internal rupture within the asphalt film with asphalt residue covering both fracture surfaces. The results indicate that temperature has a significant deteriorating effect on interfacial shear strength; as temperature rises, shear strength decays rapidly at first and then more slowly before gradually approaching zero, whilst shear fracture energy decreases monotonically, with the rate of decay being more pronounced at lower temperatures; the thickness of the bituminous film exerts a monotonically decreasing influence on interfacial shear strength, whilst shear fracture energy remains at a relatively high level within the intermediate film thickness range; bituminous films that are either too thin or too thick are detrimental to the effective dissipation of interfacial energy. An analysis of interfacial failure modes reveals that, under low-temperature conditions, failure occurs primarily at the interface between the asphalt and the aggregate, manifesting as adhesion failure; under high-temperature conditions, the failure path shifts to within the asphalt film, manifesting as cohesion failure. An increase in asphalt film thickness promotes a shift towards cohesion failure, whilst a decrease in film thickness tends to favour adhesion failure; between these two extremes, a coupled and competitive relationship is observed. Based on interfacial thermodynamics and phase transition theory, this study provides an interpretation of how temperature governs the migration of failure modes through alterations in asphalt cohesion, intermolecular forces and interfacial surface tension, and reveals the pivotal role of film thickness in restructuring the distribution of interfacial weak points by regulating the ratio of structural asphalt to free asphalt. FAU - Wang, Lina AU - Wang L AD - School of Water Conservancy and Transportation, Zhengzhou University, Zhengzhou 450001, China. FAU - Guo, Chengchao AU - Guo C AD - School of Water Conservancy and Transportation, Zhengzhou University, Zhengzhou 450001, China. AD - School of Civil Engineering, Sun Yat-sen University and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Zhuhai 519082, China. FAU - Dong, Mansheng AU - Dong M AD - School of Automotive and Transportation Engineering, Hefei University of Technology, Hefei 230002, China. FAU - Wang, Bo AU - Wang B AD - School of Water Conservancy and Transportation, Zhengzhou University, Zhengzhou 450001, China. FAU - Dong, Yuanchao AU - Dong Y AD - School of Automotive and Transportation Engineering, Hefei University of Technology, Hefei 230002, China. FAU - Zhang, Chao AU - Zhang C AD - School of Water Conservancy and Transportation, Zhengzhou University, Zhengzhou 450001, China. FAU - Gao, Yangming AU - Gao Y AD - Built Environment and Sustainable Technologies (BEST) Research Institute, Liverpool John Moores University, Liverpool L3 3AF, UK. FAU - Yang, Xiaodong AU - Yang X AUID- ORCID: 0009-0000-0586-7138 AD - School of Transportation Science and Engineering, Harbin Institute of Technology, Harbin 150096, China. LA - eng GR - 2023B82/Shandong Provincial Department of Transportation Science and Technology Project/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13706770 OTO - NOTNLM OT - SBS modified asphalt OT - adhesion failure OT - asphalt aggregate interface OT - cohesion failure OT - shear test COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:38 PHST- 2026/08/17 00:00 [received] PHST- 2026/09/13 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ma19204400 [pii] AID - materials-19-04400 [pii] AID - 10.3390/ma19204400 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 19;19(20):4400. doi: 10.3390/ma19204400. PMID- 42941130 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 19 TI - Multi-Objective Optimization of SLM Parameters for SS316L: Surface Quality, Microhardness, and Tribological Assessment. LID - 10.3390/ma19204388 [doi] LID - 4388 AB - Austenitic stainless steel 316L (SS316L) is used in orthopedic and dental implants, surgical instruments, and aerospace fluid-handling components because of its corrosion resistance and ductility. Selective Laser Melting (SLM) produces these parts with internal channels that casting and machining cannot deliver. However, as-built SS316L surfaces contain spatter, partially fused powder, and near-surface porosity that raise roughness and reduce fatigue strength, corrosion resistance, and wear performance. Producing parts that meet both surface and mechanical requirements in a single build remains difficult. This study examines how laser power, scanning speed, layer thickness, and hatch spacing affect the surface roughness, microhardness, and wear of SLM-fabricated SS316L. A Box-Behnken Design under Response Surface Methodology (RSM) was used for 27 experimental trials. Surface roughness was measured by stylus profilometry, microhardness by Vickers indentation, and dry sliding wear with a block-on-disc tribometer. Regression analysis and multi-response desirability optimization were carried out in Minitab. Processing parameters showed clear effects on surface quality and hardness; the wear response varied considerably because of localized surface defects and particle adhesion. Multi-objective optimization gave a composite desirability of 0.7603 at 260.6 W, 507.1 mm/s, 41.9 mum, and 120.0 mum. The predicted upper surface roughness, side surface roughness, and microhardness were 5.74 mum, 13.63 mum, and 210.39 HV, respectively. Run 26, tested at a volumetric energy density (VED) of 125.0 J/mm(3), recorded an upper surface roughness of 5.48 mum and a microhardness of 218.1 HV. Surface defects strongly influenced the sliding response. The study provides a practical RSM-based route for selecting SLM parameters that balance surface quality and microhardness in SS316L components. FAU - Bhatt, Darshan AU - Bhatt D AD - Department of Mechanical Engineering, School of Technology, Pandit Deendayal Energy University, Raisan, Gandhinagar 382007, India. FAU - Vora, Jay AU - Vora J AUID- ORCID: 0000-0002-7543-903X AD - Department of Mechanical Engineering, School of Technology, Pandit Deendayal Energy University, Raisan, Gandhinagar 382007, India. FAU - Sahlot, Pankaj AU - Sahlot P AUID- ORCID: 0000-0002-4759-1886 AD - Department of Mechanical Engineering, National Institute of Technology, Kurukshetra 136119, India. FAU - Patel, Sonam M AU - Patel SM AUID- ORCID: 0000-0003-2864-2550 AD - Metallurgy Department, Dr. S. & S. S. Ghandhy College of Engineering & Technology, Surat 395001, India. FAU - Parmar, Suresh F AU - Parmar SF AD - Metallurgy Department, Dr. S. & S. S. Ghandhy College of Engineering & Technology, Surat 395001, India. FAU - Kumar, K AU - Kumar K AUID- ORCID: 0009-0005-9571-9417 AD - Department of Mechanical Engineering, Inderprastha Engineering College (IPEC), Ghaziabad 201010, India. AD - Malaysian Institute of Aviation Technology (UniKL MIAT), Universiti Kuala Lumpur, Lot 2891 Jalan Jenderam Hulu, Dengkil 43800, Selangor, Malaysia. FAU - Tamyis, Najihah Mohd AU - Tamyis NM AUID- ORCID: 0000-0002-5505-5856 AD - Malaysian Institute of Aviation Technology (UniKL MIAT), Universiti Kuala Lumpur, Lot 2891 Jalan Jenderam Hulu, Dengkil 43800, Selangor, Malaysia. FAU - Bandhu, Din AU - Bandhu D AUID- ORCID: 0000-0001-9163-353X AD - Department of Mechanical Engineering, Additive Manufacturing Research and Innovation Center (AMRIC), College of Engineering, Prince Mohammad Bin Fahd University, Al-Khobar 31952, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13706898 OTO - NOTNLM OT - RSM OT - SLM OT - SS316L OT - additive manufacturing OT - microhardness OT - process optimization OT - surface roughness OT - wear COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:38 PHST- 2026/09/27 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ma19204388 [pii] AID - materials-19-04388 [pii] AID - 10.3390/ma19204388 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 19;19(20):4388. doi: 10.3390/ma19204388. PMID- 42941123 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 19 TI - Phase-Selective Corrosion Kinetics of Marine Copper Alloys Under High-Speed Dynamic Seawater Erosion. LID - 10.3390/ma19204381 [doi] LID - 4381 AB - Nickel-aluminum bronze is a key structural material for marine propellers, yet its corrosion behavior under dynamic seawater erosion remains inadequately elucidated. To clarify the corrosion kinetics under high-speed dynamic seawater erosion of the ZCuAl10-4-4-2 marine copper alloy, erosion-corrosion tests and multi-method characterizations were conducted. The results illustrate that the alpha phase exhibited a higher corrosion susceptibility, driving micro-damage evolution: kappa/alpha interface crack initiation at 4 h, significant crack propagation with a dense kappa(II) phase at 24 h, and pit formation ( approximately 100 mum) at 48 h. Dynamic erosion induced critical electrochemical changes at 4 h and 24 h, with reduced charge transfer resistance, lower corrosion potential (E(corr)) and elevated current density; the 24 h stage showed the peak corrosion rate due to unstable, spallable corrosion product films. Prolonged erosion-corrosion promoted the formation of a stable protective corrosion product layer, which enhanced the alloy's corrosion resistance and reduced its corrosion rate. FAU - Hao, Xuelong AU - Hao X AD - National Center of Analysis and Testing for Nonferrous Metals and Electronic Materials, China GRINM Group Co., Ltd., Beijing 100088, China. AD - Guobiao (Beijing) Testing & Certification Co., Ltd., Beijing 101407, China. FAU - Tang, Jiangda AU - Tang J AD - National Center of Analysis and Testing for Nonferrous Metals and Electronic Materials, China GRINM Group Co., Ltd., Beijing 100088, China. AD - Guobiao (Beijing) Testing & Certification Co., Ltd., Beijing 101407, China. FAU - Tian, Yongjun AU - Tian Y AD - Fastener Group (Guangdong) Co., Ltd., Guangzhou 215155, China. FAU - Zhang, Jingtong AU - Zhang J AD - National Center of Analysis and Testing for Nonferrous Metals and Electronic Materials, China GRINM Group Co., Ltd., Beijing 100088, China. AD - Guobiao (Beijing) Testing & Certification Co., Ltd., Beijing 101407, China. FAU - Huang, Shuhui AU - Huang S AD - State Key Laboratory of Nonferrous Structural Materials, China GRINM Group Co., Ltd., Beijing 100088, China. AD - GRIMAT Engineering Institute Co., Ltd., Beijing 101407, China. FAU - Fan, Lei AU - Fan L AD - School of Mechanical and Electrical Engineering, China University of Mining and Technology-Beijing, Beijing 100083, China. AD - Key Laboratory of Intelligent Mining and Robotics, Ministry of Emergency Management, Beijing 100083, China. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707327 OTO - NOTNLM OT - erosion-corrosion OT - marine copper alloy OT - microscopic damage OT - simulation of the ocean COIS- Authors XueLong Hao, JiangDa Tang and JingTong Zhang were employed by Guobiao (Beijing) Testing & Certification Company, Ltd., General Research Institute for Nonferrous Metals (China), Beijing, China. Author YongJun Tian was employed by Fastener Group (Guangdong) Company, Ltd., Guangdong, China. Author ShuHui Huang was employed by GRIMAT Engineering Institute Company, Ltd., State Key Laboratory of Nonferrous Structural Materials, China, and GRINM Group Company, Ltd., Beijing 100088, Beijing, China. The remaining author declared that the research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:38 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ma19204381 [pii] AID - materials-19-04381 [pii] AID - 10.3390/ma19204381 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 19;19(20):4381. doi: 10.3390/ma19204381. PMID- 42941119 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 18 TI - Chromium-Decorated Double-Walled Carbon Nanotubes for Preferential Room-Temperature Xylene Sensing. LID - 10.3390/ma19204378 [doi] LID - 4378 AB - Room-temperature discrimination of closely related aromatic volatile organic compounds remains challenging for chemiresistive sensors. Here, chromium-modified double-walled carbon nanotubes (Cr-DWNTs) were prepared using an endohedral filling process and were investigated for preferential xylene detection. X-ray diffraction showed substantial transformation of the CrCl(2) precursor to rhombohedral Cr(2)O(3) identified as the dominant crystalline chromium-containing phase. Transmission electron microscopy and elemental analysis supported association of the chromium-containing phase with the DWNT structure. Raman spectroscopy showed retention of the characteristic D, G, and 2D bands with only a small change in the ID/IG ratio, indicating preservation of the graphitic framework after chromium modification. Hall-effect measurements showed positive Hall coefficients for both pristine DWNTs and Cr-DWNTs, confirming hole-dominated transport, while Cr-DWNTs exhibited a lower apparent Hall carrier concentration together with substantially higher carrier mobility and conductivity. Cr-DWNTs exhibited a pronounced preferential response toward xylene over 0.1-10 ppm. The xylene response increased from approximately 0.96% at 0.1 ppm to 5.93% at 10 ppm and was enhanced by approximately 1.9-2.6 times relative to pristine DWNTs. Within 0.1-1 ppm, the low-concentration xylene sensitivity was 2.36% ppm(-1) (R2=0.958), with a calculated limit of detection of approximately 37 ppb. The enhanced response is attributed to the combination of the intrinsic affinity of graphitic carbon toward methyl-substituted aromatics and interfacial electronic transduction within the DWNT network. These results demonstrate that chromium modification provides an effective strategy for amplifying room-temperature xylene selectivity in CNT-based chemiresistive sensors. FAU - Setlhare, Tlotlo Cassandra AU - Setlhare TC AUID- ORCID: 0009-0000-1048-4860 AD - Department of Physics and Astronomy, Botswana International University of Science and Technology, Boseja Ward, Palapye Private Bag 16, Botswana. FAU - Mpolokang, Atlang Gild AU - Mpolokang AG AUID- ORCID: 0000-0003-3527-9809 AD - Department of Physics and Astronomy, Botswana International University of Science and Technology, Boseja Ward, Palapye Private Bag 16, Botswana. FAU - Flahaut, Emmanuel AU - Flahaut E AUID- ORCID: 0000-0001-8344-6902 AD - Universite de Toulouse, Toulouse INP, CNRS, CIRIMAT, Toulouse, France. FAU - Figueiredo, Martin AU - Figueiredo M AUID- ORCID: 0009-0000-6620-0425 AD - Universite de Toulouse, Toulouse INP, CNRS, CIRIMAT, Toulouse, France. FAU - Chimowa, George AU - Chimowa G AUID- ORCID: 0000-0002-7421-1068 AD - Department of Physics and Astronomy, Botswana International University of Science and Technology, Boseja Ward, Palapye Private Bag 16, Botswana. LA - eng GR - DCI-PANAF/2020/420-028/African Academy of Sciences/ PT - Journal Article DEP - 20261018 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707281 OTO - NOTNLM OT - BTX discrimination OT - chemiresistive gas sensor OT - chromium(II) chloride OT - double-walled carbon nanotubes OT - endohedral filling OT - volatile organic compounds OT - xylene sensing COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:38 PHST- 2026/09/04 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - ma19204378 [pii] AID - materials-19-04378 [pii] AID - 10.3390/ma19204378 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 18;19(20):4378. doi: 10.3390/ma19204378. PMID- 42941118 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 18 TI - Solvent-Cast PCL/Hemp Biocomposites: Screening of the Effects of Matrix Grade, Filler Content and Particle Size. LID - 10.3390/ma19204377 [doi] LID - 4377 AB - Biodegradable poly(epsilon-caprolactone) (PCL)/hemp biocomposites were prepared by solvent casting in acetic acid to screen the effects of matrix grade, filler content, and particle size on tensile behavior. Two PCL grades (Capa((R)) 6500 and 6800) were combined with milled hemp sieved at four sizes (0.08 to 1.0 mm) and two contents (20 and 30 wt%), giving 13 formulations (86 specimens), analyzed statistically and with a combined Pukanszky-Weibull model. Particle size produced a plateau: the 0.08 and 0.25 mm fractions were equivalent (p(adj) > 0.25), with a marked drop at 0.5 mm. Raising the content to 30 wt% lowered the strength 2.5 to 4 times (p < 0.001), showing that hemp acts mainly as a filler. For PCL 6800 at 20 wt%, the modulus at the two finest sizes exceeded the neat value, a mild reinforcement described by a Halpin-Tsai term. A low Weibull modulus (m(W) approximately 3) indicated defect-controlled behavior, and electron microscopy showed matrix voids, more pronounced for PCL 6800, and interfacial gaps. Thermal analyses indicated limited matrix degradation during dissolution and unchanged thermal stability. PCL 6800 with 20 wt% hemp at 0.25 mm performed best. This feasibility screening supports formulation selection for a planned melt processing phase. FAU - Lupia, Gaia Francesca AU - Lupia GF AUID- ORCID: 0009-0007-9788-1033 AD - Department of Chemical Engineering, Materials, Environment, Sapienza University of Rome, 00184 Rome, Italy. AD - INSTM Reference Laboratory for Engineering of Surface Treatments, UdR Rome, Sapienza University of Rome, 00184 Rome, Italy. FAU - Pini, Tommaso AU - Pini T AUID- ORCID: 0000-0002-0615-9384 AD - Department of Chemical Engineering, Materials, Environment, Sapienza University of Rome, 00184 Rome, Italy. AD - INSTM Reference Laboratory for Engineering of Surface Treatments, UdR Rome, Sapienza University of Rome, 00184 Rome, Italy. FAU - Colella, Paola Francesca AU - Colella PF AD - Department of Chemical Engineering, Materials, Environment, Sapienza University of Rome, 00184 Rome, Italy. FAU - Valente, Marco AU - Valente M AUID- ORCID: 0000-0002-6298-3693 AD - Department of Chemical Engineering, Materials, Environment, Sapienza University of Rome, 00184 Rome, Italy. AD - INSTM Reference Laboratory for Engineering of Surface Treatments, UdR Rome, Sapienza University of Rome, 00184 Rome, Italy. LA - eng PT - Journal Article DEP - 20261018 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707278 OTO - NOTNLM OT - PCL OT - biocomposites OT - defect-controlled behavior OT - hemp OT - lignocellulosic filler OT - particle size effect OT - solvent casting COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:38 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - ma19204377 [pii] AID - materials-19-04377 [pii] AID - 10.3390/ma19204377 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 18;19(20):4377. doi: 10.3390/ma19204377. PMID- 42941117 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 18 TI - Feasibility Study of a Novel Post-Processing Method Based on Microscale Laser Shock Peening Without Coating for Selective Laser Melting-Fabricated Components. LID - 10.3390/ma19204376 [doi] LID - 4376 AB - Selective Laser Melting (SLM) has been widely employed for fabricating lightweight and integrated components for aircraft and aero-engine applications. However, SLM-fabricated components, especially those containing locally complex surface regions, often suffer from burrs, balling, microcracks, and tensile residual stress (TRS). These issues are difficult to eliminate by conventional machining or heat treatment (HT) alone. Consequently, such defects degrade fatigue performance and restrict the practical engineering application of SLM-fabricated components. This study proposed a novel direct surface post-processing method utilizing microscale laser shock peening without coating (muLSPwC) for locally complex surface regions of SLM-fabricated components. The process was designed to combine laser-induced local micro-melting for surface defect mitigation with shock-induced plastic deformation for near-surface strengthening. SLM-fabricated 17-4PH (AISI 630, UNS S17400) stainless steel, TC4 (Ti-6Al-4V) titanium alloy, and GH4169 (Inconel 718, UNS N07718) nickel-based superalloy were treated to assess the impact of muLSPwC on surface morphology, roughness, residual stress, microhardness, and rotating bending fatigue life. The surface roughness of all three materials dropped more than 20% after treatment. Furthermore, muLSPwC introduced compressive residual stress (CRS) layers and hardened layers exceeding 300 mum. The rotating bending fatigue life was improved by more than 20 times, and in some cases by over two orders of magnitude, at the selected stress levels. Therefore, muLSPwC is a promising post-processing technique for locally complex surface regions on SLM-fabricated components, particularly in situations where hot isostatic pressing, HT, or conventional surface machining is impractical or ineffective. FAU - Shi, Congkai AU - Shi C AUID- ORCID: 0009-0009-9471-6942 AD - National Key Laboratory of Aerospace Power System and Plasma Technology, Air Force Engineering University, Xi'an 710038, China. FAU - Nie, Xiangfan AU - Nie X AD - National Key Laboratory of Aerospace Power System and Plasma Technology, Air Force Engineering University, Xi'an 710038, China. AD - National Key Laboratory of Aerospace Power System and Plasma Technology, Xi'an Jiaotong University, Xi'an 710049, China. FAU - Shu, Song AU - Shu S AD - State-Owned Machinery Factory in Wuhu, Wuhu 241007, China. FAU - Cui, Rubin AU - Cui R AD - National Key Laboratory of Aerospace Power System and Plasma Technology, Xi'an Jiaotong University, Xi'an 710049, China. FAU - Li, Hongbing AU - Li H AD - National Key Laboratory of Aerospace Power System and Plasma Technology, Xi'an Jiaotong University, Xi'an 710049, China. FAU - Li, Shixi AU - Li S AD - National Key Laboratory of Aerospace Power System and Plasma Technology, Xi'an Jiaotong University, Xi'an 710049, China. FAU - Xu, Peiwen AU - Xu P AD - School of Aeronautics, Chongqing Jiaotong University, Chongqing 400074, China. LA - eng GR - SXQNBJ-2023016/the Young Talents Support Program of Shaanxi Province/ GR - 2024-QN08137/the Military Youth Science and Technology Talent Project/ GR - 2026JC-JCQN-057/Category A Youth Project of Shaanxi Provincial Natural Science Basic Research Program/ PT - Journal Article DEP - 20261018 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707067 OTO - NOTNLM OT - fatigue performance OT - micro-melting effect OT - microscale laser shock peening without coating OT - selective laser melting OT - severe plastic deformation OT - surface integrity COIS- Author Song Shu was employed by the company State-Owned Machinery Factory in Wuhu. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:38 PHST- 2026/08/11 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - ma19204376 [pii] AID - materials-19-04376 [pii] AID - 10.3390/ma19204376 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 18;19(20):4376. doi: 10.3390/ma19204376. PMID- 42941116 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 18 TI - Experimental Study of Surface Reinforcement of Soil Slopes Using Carbon Fiber-Reinforced Stabilized Soil. LID - 10.3390/ma19204375 [doi] LID - 4375 AB - Rainfall-induced erosion and deformation of soil slopes remain important concerns for long-term slope stability, highlighting the need for effective surface reinforcement methods. This study experimentally investigated the mechanical and rainfall-resistant performance of carbon fiber-reinforced stabilized soil for slope surface protection. Four soil types were initially evaluated using unconfined compression tests with different Eco Soil Binder (E.S.B.) contents, and well-graded sand (SW) with 20% E.S.B. was selected for further testing. Carbon fiber contents ranging from 0 to 1.2% were considered; however, stable specimens could not be formed at 1.2%, while 0.6% provided the highest compressive strength among the valid test conditions. The selected mixture of SW, 20% E.S.B., and 0.6% carbon fiber was further characterized through triaxial compression tests, yielding an apparent cohesion of 0.152 MPa and a friction angle of 37.23 degrees . Artificial rainfall tests were then conducted using 1:50 geometrically scaled slope models with berm lengths of 0.05 and 0.15 m under a rainfall intensity of 20 mm/h for 48 h. Under the tested small-scale conditions, the stabilized slope models exhibited approximately 47-60% lower settlement than the unreinforced models, together with lower final moisture contents and less visible surface deterioration. Because the two model configurations differed in both material composition and initial unit weight, these results should be interpreted as descriptive comparative observations rather than as isolated effects of E.S.B. and carbon fiber. FAU - Oh, Sewook AU - Oh S AD - Department of Construction and Disaster Prevention Engineering, Kyungpook National University, Gyeongbuk 37224, Republic of Korea. FAU - Yang, Sunghwan AU - Yang S AD - Department of Construction and Disaster Prevention Engineering, Kyungpook National University, Gyeongbuk 37224, Republic of Korea. FAU - Kim, Donggeun AU - Kim D AD - Department of Forest Ecology and Protection, Kyungpook National University, Gyeongbuk 37224, Republic of Korea. FAU - Jeong, Yoseok AU - Jeong Y AUID- ORCID: 0000-0003-3478-7297 AD - Department of Construction and Disaster Prevention Engineering, Kyungpook National University, Gyeongbuk 37224, Republic of Korea. LA - eng GR - RS-2025-02223388/Korea Forest Service/ PT - Journal Article DEP - 20261018 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707326 OTO - NOTNLM OT - artificial rainfall OT - carbon fiber reinforcement OT - erosion resistance OT - rainfall-induced deformation OT - slope protection OT - soil stabilization COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:38 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - ma19204375 [pii] AID - materials-19-04375 [pii] AID - 10.3390/ma19204375 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 18;19(20):4375. doi: 10.3390/ma19204375. PMID- 42941111 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 17 TI - Topology-Dependent Modeling Fidelity of Lattice-Reinforced UAV Leading-Edge Structures Under Bird Strike Impact. LID - 10.3390/ma19204370 [doi] LID - 4370 AB - Lattice-reinforced thin-walled structures offer a promising means of improving impact resistance while maintaining structural efficiency; however, their detailed numerical simulation can become computationally expensive under high-rate loading conditions. This study investigates how lattice topology influences the fidelity of reduced-order beam element models for bird strike impact analysis. Three lattice topologies, BCC, Diamond, and Kelvin, are evaluated using low-fidelity beam element and high-fidelity solid element models under identical geometry, material properties, boundary conditions, and impact loading at 68.4 m/s. The comparison considers transient and residual displacement, total and component-level energy absorption, stress distribution, and computational cost. The results demonstrate that modeling fidelity is strongly topology-dependent and response-dependent. For the sparse BCC architecture, low-fidelity predictions agree closely with the high-fidelity reference, with peak displacement and total energy discrepancies of approximately 10% and below 5%, respectively. In contrast, Kelvin and Diamond topologies exhibit larger discrepancies, particularly in residual deformation and component-level energy redistribution. Notably, the magnitude and character of the fidelity discrepancy vary with topology and response quantity, indicating that a universal correction factor based on a single response metric is inappropriate. Despite these limitations, beam element models reduce computational time by 74-86% relative to their solid element counterparts. The results provide quantitative guidance for selecting modeling fidelity according to lattice topology and structural response quantity within the investigated bird strike configuration. FAU - Muneer, Muhammad Arslan AU - Muneer MA AUID- ORCID: 0009-0008-6184-262X AD - Department of Engineering, University of Campania "Luigi Vanvitelli", Via Roma 29, 81031 Aversa, Italy. FAU - Garofano, Antonio AU - Garofano A AUID- ORCID: 0000-0002-0230-0539 AD - Department of Engineering, University of Campania "Luigi Vanvitelli", Via Roma 29, 81031 Aversa, Italy. FAU - Riccio, Aniello AU - Riccio A AUID- ORCID: 0000-0001-7426-6803 AD - Department of Engineering, University of Campania "Luigi Vanvitelli", Via Roma 29, 81031 Aversa, Italy. LA - eng PT - Journal Article DEP - 20261017 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13706944 OTO - NOTNLM OT - beam and solid finite elements OT - bird strike impact OT - computational mechanics OT - lattice structures OT - multi-fidelity modeling OT - topology-dependent response COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:38 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - ma19204370 [pii] AID - materials-19-04370 [pii] AID - 10.3390/ma19204370 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 17;19(20):4370. doi: 10.3390/ma19204370. PMID- 42941105 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 16 TI - Aging Resistance Improvement of Rubber-Plastic-Based TPE-Modified Asphalt System with Different Plastic Types. LID - 10.3390/ma19204364 [doi] LID - 4364 AB - This study evaluated the aging resistance of bitumen modified with integrated rubber-plastic thermoplastic elastomers (TPEs). Surface-activated low-Mooney reclaimed rubber was compounded with recycled high-density polyethylene, low-density polyethylene, or polypropylene by twin-screw reactive extrusion using a maleic anhydride-grafted styrenic block copolymer compatibilizer. Reference binders contained styrene-butadiene-styrene (SBS), rubber alone, or individual polyolefins. Aging comprised the rolling thin-film oven test, pressure aging vessel conditioning of oven-aged residues, and ultraviolet exposure. Dynamic shear rheometry, Fourier transform infrared spectroscopy, and atomic force microscopy characterized rheological, chemical, and topographic changes. The largest rutting factor and carbonyl aging indices occurred after sequential oven and pressure aging under the selected conditions. The TPE-modified binders showed lower rutting factor and carbonyl aging indices and smaller relative roughness increases than the SBS reference under each protocol. Among the three TPE formulations, the low-density polyethylene composite had the lowest rutting factor aging indices, whereas the high-density polyethylene composite had the lowest carbonyl aging indices and relative roughness increases after pressure and ultraviolet aging. These findings support the laboratory aging-resistance potential of integrated TPEs, with improved interfacial compatibility providing a plausible explanation. The approach offers a route for the high-value utilization of waste rubber and plastics. FAU - Zhang, Liuyang AU - Zhang L AD - Guangxi Key Laboratory of Road Structure and Materials, Nanning 530007, China. AD - School of Traffic and Transportation Engineering, Changsha University of Science & Technology, Changsha 410004, China. FAU - Yi, Keliang AU - Yi K AD - Guangxi Key Laboratory of Road Structure and Materials, Nanning 530007, China. AD - Guangxi Transportation Science and Technology Group Co., Ltd., Nanning 530007, China. FAU - Xiong, Jianping AU - Xiong J AD - Guangxi Key Laboratory of Road Structure and Materials, Nanning 530007, China. AD - Guangxi Transportation Science and Technology Group Co., Ltd., Nanning 530007, China. FAU - Mao, Nana AU - Mao N AD - School of Traffic and Transportation Engineering, Changsha University of Science & Technology, Changsha 410004, China. FAU - He, Peikai AU - He P AD - Hebei Communications Investment Resource Development and Utilization Co., Ltd., Shijiazhuang 050000, China. FAU - Xu, Peng AU - Xu P AUID- ORCID: 0009-0002-6238-2596 AD - School of Traffic and Transportation Engineering, Changsha University of Science & Technology, Changsha 410004, China. FAU - Yang, Hao AU - Yang H AD - Hebei Communications Investment Resource Development and Utilization Co., Ltd., Shijiazhuang 050000, China. FAU - Zhou, Hongyu AU - Zhou H AD - School of Traffic and Transportation Engineering, Changsha University of Science & Technology, Changsha 410004, China. FAU - Ma, Yuetan AU - Ma Y AD - School of Traffic and Transportation Engineering, Changsha University of Science & Technology, Changsha 410004, China. LA - eng GR - 52308437/National Natural Science Foundation of China/ GR - 52478441/National Natural Science Foundation of China/ GR - 2025JJ60273/Natural Science Foundation of Hunan Province/ GR - 2026JJ50192/Natural Science Foundation of Hunan Province/ GR - 2024YFB2605101/National Key Research and Development Program of China/ GR - 2021gxjgclkf001/Open Fund of Guangxi Key Laboratory of Road Structure and Materials/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13706713 OTO - NOTNLM OT - aging resistance OT - modified bitumen OT - multiscale characterization OT - reactive extrusion OT - reclaimed rubber OT - recycled polyolefins COIS- Authors Peikai He and Hao Yang were employed by Hebei Communications Investment Resource Development and Utilization Co., Ltd. Authors Keliang Yi and Jianping Xiong were employed by Guangxi Transportation Science and Technology Group Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:38 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ma19204364 [pii] AID - materials-19-04364 [pii] AID - 10.3390/ma19204364 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 16;19(20):4364. doi: 10.3390/ma19204364. PMID- 42941101 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 16 TI - Research Progress on Offline Defect-Detection Technologies for Selective Laser-Melting Parts. LID - 10.3390/ma19204360 [doi] LID - 4360 AB - Selective laser melting (SLM) is recognized as one of the most promising metal additive manufacturing technologies for industrial deployment and has been applied to fabricate precision components for high-end equipment in sectors including aerospace and biomedicine. Nevertheless, ensuring reliable component quality and consistent performance remain a core challenge for SLM, which is widely acknowledged as a major barrier restricting the large-scale industrial application of the technology. Multi-scale and multi-type defects, which are difficult to fully suppress during the forming process, are the primary contributing factors to this issue. Accordingly, research on offline defect-detection technologies for SLM components is a critical pathway to break through the quality bottleneck and has emerged as a prominent research focus in both academia and industry. Based on a systematic review of relevant literature published in the past five years, this paper summarizes the morphological characteristics and formation mechanisms of typical defects in SLM-fabricated parts, including porosity, cracks, inclusions, and surface-forming defects. It elaborates on mainstream offline detection methods such as ultrasonic testing, X-ray computed tomography (XCT), and deep learning-enabled intelligent detection, and clarifies the respective performance advantages and inherent limitations of each technology. A quantitative comparison of these technologies is conducted from three core dimensions, detection accuracy, detection efficiency, and deployment cost, and differentiated application scenarios are defined corresponding to defect characteristics. Finally, the paper concludes with a full summary and prospects future in-depth research directions and industrial development trends in the field of SLM offline defect detection. FAU - Tian, Chenchen AU - Tian C AD - School of Mechanical Engineering, Shandong University, Jinan 250061, China. AD - Key Laboratory of High Efficiency and Clean Mechanical Manufacture of Ministry of Education, Shandong University, Jinan 250061, China. AD - National Key Laboratory of High-end Equipment and Advanced Technology for Metal Forming, Shandong University, Jinan 250061, China. FAU - Xu, Kai AU - Xu K AUID- ORCID: 0009-0008-1016-5710 AD - School of Mechanical Engineering, Shandong University, Jinan 250061, China. AD - Key Laboratory of High Efficiency and Clean Mechanical Manufacture of Ministry of Education, Shandong University, Jinan 250061, China. AD - National Key Laboratory of High-end Equipment and Advanced Technology for Metal Forming, Shandong University, Jinan 250061, China. FAU - Wu, Zikui AU - Wu Z AD - School of Mechanical Engineering, Shandong University, Jinan 250061, China. AD - Key Laboratory of High Efficiency and Clean Mechanical Manufacture of Ministry of Education, Shandong University, Jinan 250061, China. AD - National Key Laboratory of High-end Equipment and Advanced Technology for Metal Forming, Shandong University, Jinan 250061, China. FAU - Li, Xuekun AU - Li X AD - Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China. LA - eng GR - 2024YFB4608900)/National Key Research and Development Program of China/ GR - tsqn202408010/TaiShan Academic Youth Expert Program/ GR - Enterprise Project/ PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707165 OTO - NOTNLM OT - XCT OT - defect detection OT - selective laser melting OT - ultrasonic testing COIS- The authors declare that this study received funding from Enterprise Project (Jiangsu Saiyang Precision Technology Co., Ltd.). The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article, or the decision to submit it for publication. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:38 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ma19204360 [pii] AID - materials-19-04360 [pii] AID - 10.3390/ma19204360 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 16;19(20):4360. doi: 10.3390/ma19204360. PMID- 42941096 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 16 TI - Shaping Nematic Liquid Crystal Flow States with In-Plane Electric Fields. LID - 10.3390/ma19204355 [doi] LID - 4355 AB - We demonstrate electrical control of flow states in a nematic liquid crystal driven through microfluidic channels. Patterned electrodes generate in-plane fields using a 1 Hz bipolar square-wave drive with peak amplitudes of 3.5 to 9 V. At flow velocities of 50 to 80 mums-1, these fields reversibly reshape flowing dowser domains, split them into smaller domains, and nucleate new dowser or chiral domains. Field orientation and flow conditions select between domain pairs, individual domains and chiral streams, with nucleation intervals of 0.5 to 2 s under steady flow. Combining the field with pulsating flow produces larger individual domains. These results establish patterned electrodes as localized electrical triggers for controlling nematic flow states, with prospects for integration in optofluidic and lab-on-a-chip devices. FAU - Emersic, Tadej AU - Emersic T AUID- ORCID: 0000-0002-6084-5182 AD - Department of Physics and Materials Science, Faculty of Science, Technology and Medicine, University of Luxembourg, 162a Avenue de la Faiencerie, L-1511 Luxembourg, Luxembourg. FAU - Tkalec, Uros AU - Tkalec U AUID- ORCID: 0000-0003-0552-0518 AD - Institute of Biophysics, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia. AD - Department of Condensed Matter Physics, Jozef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia. AD - Faculty of Natural Sciences and Mathematics, University of Maribor, Koroska 160, 2000 Maribor, Slovenia. LA - eng GR - P1-0055/The Slovenian Research and Innovation Agency/ GR - J2-50092/The Slovenian Research and Innovation Agency/ GR - N1-0435/The Slovenian Research and Innovation Agency/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707002 OTO - NOTNLM OT - dowser texture OT - electric field OT - flow OT - interdigitated electrodes OT - liquid crystals OT - microfluidics OT - topological defects COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:38 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ma19204355 [pii] AID - materials-19-04355 [pii] AID - 10.3390/ma19204355 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 16;19(20):4355. doi: 10.3390/ma19204355. PMID- 42941093 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 16 TI - Experimental and Statistical Analyses of Dimensional Accuracy of Geometry-Dependent Features in Fiber Laser Cutting of CFRP. LID - 10.3390/ma19204352 [doi] LID - 4352 AB - This study investigates the influence of fiber laser cutting parameters on the dimensional accuracy of carbon fiber-reinforced polymer (CFRP) components. A full factorial design of experiments was employed considering laser power, cutting speed, and laser operating mode as input factors. Three laser power levels (800, 1600, and 2000 W), three cutting speeds (250, 500, and 1000 mm/min), and two operating modes (continuous wave, CW, and gated pulse, GP) were evaluated. Dimensional accuracy of 90 laser-cut specimens was assessed using three selected geometrical responses-the inner width (L(2)) and two internal feature heights (a(1) and a(3))-followed by analysis of variance and regression modeling. Laser power was identified as the dominant factor for L(2) and a(1), whereas a(3) exhibited pronounced nonlinear behavior governed primarily by cutting speed and its quadratic contribution. The operating mode significantly affected L(2) and a(3), while its influence on a(1) was statistically insignificant. The pronounced nonlinear behavior of a(3) may be associated with the combined effects of heat accumulation, anisotropic heat transfer through the carbon-fiber reinforcement, and thermally induced degradation and recession of the polymer matrix. Differences in temporal energy delivery between CW and GP operation may influence the local thermal conditions and consequently affect material removal and the resulting position of the cut edge. The developed regression models achieved R(2) values of 90.37%, 87.25%, and 92.22% for L(2), a(1), and a(3), respectively. The calculated nominal line energy ranged from 48 to 480 J/mm and supported the observed tendency that higher power combined with lower cutting speed was generally associated with increased dimensional deviation. The results demonstrate that dimensional accuracy in fiber laser cutting of CFRP is strongly geometry-dependent and requires feature-specific process optimization rather than a single universal cutting condition. FAU - Cacko, Viliam AU - Cacko V AUID- ORCID: 0000-0002-7210-5106 AD - Institute of Production Engineering and Quality, Faculty of Mechanical Engineering, Slovak University of Technology in Bratislava, Nam. slobody 17, 812 31 Bratislava, Slovakia. FAU - Cackova, Iveta AU - Cackova I AUID- ORCID: 0000-0002-3402-7071 AD - Institute of Production Engineering and Quality, Faculty of Mechanical Engineering, Slovak University of Technology in Bratislava, Nam. slobody 17, 812 31 Bratislava, Slovakia. FAU - Ferenczi, Balint AU - Ferenczi B AUID- ORCID: 0009-0006-3250-2592 AD - Institute of Production Engineering and Quality, Faculty of Mechanical Engineering, Slovak University of Technology in Bratislava, Nam. slobody 17, 812 31 Bratislava, Slovakia. FAU - Shabu, Shane AU - Shabu S AUID- ORCID: 0009-0008-6667-7467 AD - Institute of Production Engineering and Quality, Faculty of Mechanical Engineering, Slovak University of Technology in Bratislava, Nam. slobody 17, 812 31 Bratislava, Slovakia. FAU - Brusilova, Alena AU - Brusilova A AD - Institute of Production Engineering and Quality, Faculty of Mechanical Engineering, Slovak University of Technology in Bratislava, Nam. slobody 17, 812 31 Bratislava, Slovakia. FAU - Gabrisova, Zuzana AU - Gabrisova Z AUID- ORCID: 0000-0003-3683-1220 AD - Institute of Production Engineering and Quality, Faculty of Mechanical Engineering, Slovak University of Technology in Bratislava, Nam. slobody 17, 812 31 Bratislava, Slovakia. FAU - Soos, Lubomir AU - Soos L AUID- ORCID: 0000-0003-3161-5609 AD - Institute of Production Engineering and Quality, Faculty of Mechanical Engineering, Slovak University of Technology in Bratislava, Nam. slobody 17, 812 31 Bratislava, Slovakia. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13706935 OTO - NOTNLM OT - CFRP OT - cutting speed OT - design of experiments OT - dimensional accuracy OT - fiber laser cutting OT - laser power OT - operating mode OT - regression modeling COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:38 PHST- 2026/09/18 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ma19204352 [pii] AID - materials-19-04352 [pii] AID - 10.3390/ma19204352 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 16;19(20):4352. doi: 10.3390/ma19204352. PMID- 42941087 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 15 TI - Comparative Physical and Mechanical Characterization of Lightweight Porous Materials: Bulk Density as Indicator of Mechanical Properties. LID - 10.3390/ma19204346 [doi] LID - 4346 AB - Lightweight porous materials are widely used in construction due to their low density and favorable thermal insulation properties; however, their mechanical performance depends on factors extending beyond bulk density alone. The aim of this study was to perform a comprehensive physical and mechanical characterization of three representative lightweight porous materials belonging to different material classes: autoclaved aerated concrete (AAC), foamed concrete, and rigid polyurethane foam, within a common experimental and analytical framework. The investigation included the determination of particle density, bulk density, porosity, unconfined compressive strength (UCS), Young's modulus, and shear strength parameters obtained from triaxial compression tests. In addition, correlations between bulk density and selected mechanical properties were analyzed for each material. The mean bulk density, porosity, and UCS values were 566 kg/m(3), 73%, and 5.24 MPa for AAC; 518 kg/m(3), 77%, and 0.88 MPa for foamed concrete; and 82 kg/m(3), 93%, and 0.52 MPa for rigid polyurethane foam, respectively. Despite similar bulk densities, AAC and foamed concrete differed by approximately sixfold in UCS, eightfold in Young's modulus, and sixfold in cohesion. Correlation analyses further showed that the density-property relationships were material-specific. The findings indicate that bulk density alone is not a universal predictor of the mechanical behavior of different classes of lightweight porous materials. A comprehensive characterization combining physical and mechanical properties is therefore required for reliable material evaluation and comparison. FAU - Kolano, Malwina AU - Kolano M AUID- ORCID: 0000-0001-6341-4954 AD - Faculty of Civil Engineering and Resource Management, AGH University of Krakow, 30-059 Krakow, Poland. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707320 OTO - NOTNLM OT - autoclaved aerated concrete OT - bulk density OT - foamed concrete OT - lightweight porous materials OT - mechanical properties OT - rigid polyurethane foam COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:38 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ma19204346 [pii] AID - materials-19-04346 [pii] AID - 10.3390/ma19204346 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 15;19(20):4346. doi: 10.3390/ma19204346. PMID- 42941082 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 15 TI - Cross-Alloy Transferability of Spindle-Synchronous Vibration Signatures in CNC Milling Using Physics-Informed Autospectrum Features. LID - 10.3390/ma19204341 [doi] LID - 4341 AB - Data-driven recognition of machining conditions in CNC milling processes represents a significant research focus within process monitoring applications. This work assesses whether physics-informed, spindle-synchronous vibration representations remain transferable between Al 6061 and Al 7075 using multi-axis and multi-location autospectrum data. Spindle speed is used as a controlled and physically traceable process-state label rather than as an unknown CNC variable to be reconstructed. Vibration signals were collected under various combinations of feed rate, spindle speed, and depth of cut. Subsequently, autospectrum descriptors associated with spindle rotation, harmonic components, frequency-band energy, and sensor location were extracted. Preliminary cross-alloy analysis demonstrated that spindle speed recognition exhibited more stable transfer performance than feed rate or depth of cut recognition. Multiple machine learning models were evaluated, with the combination of spindle-related autospectrum descriptors + LightGBM model selected as the final configuration. The resulting model achieved 0.8708 accuracy, 0.8701 Macro-F1, 0.7500 MCC, and 0.9615 ROC-AUC in the cross-alloy spindle speed recognition task. In two-way transfer analyses, Macro-F1 scores of 0.8748 and 0.8661 were obtained for the Al 6061 to Al 7075 and Al 7075 to Al 6061 scenarios, respectively. Ablation studies indicated that spindle-related frequency descriptors offer advantages over comprehensive feature representations. SHAP-based explainability analysis revealed that harmonic band, tooth-passing band, and sensor-location-dependent spectral features were most influential in model decisions. These findings indicate that physics-informed autospectrum descriptors preserve discriminative spindle-synchronous vibration information across the investigated Al 6061 and Al 7075 alloy domains. FAU - Isci, Muhammed AU - Isci M AUID- ORCID: 0000-0003-2319-6379 AD - Mechatronics Program, Department of Machine and Metal Technologies, Technical Sciences Vocational School, Kayseri University, Kayseri 38280, Turkey. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13706887 OTO - NOTNLM OT - Al 6061/7075 OT - CNC milling OT - autospectrum OT - explainable ML OT - spindle speed OT - vibration response COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:38 PHST- 2026/09/13 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ma19204341 [pii] AID - materials-19-04341 [pii] AID - 10.3390/ma19204341 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 15;19(20):4341. doi: 10.3390/ma19204341. PMID- 42941078 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 15 TI - Effect of the MOPA Laser Cleaning Parameters on the Surface Evolution of the Epoxy Coating on AH36 Steel. LID - 10.3390/ma19204338 [doi] LID - 4338 AB - High-power laser cleaning has been extensively adopted to remove organic coatings, but pulse duration, pulse energy, and overlap rate must be adjusted to limit residue and thermally affected surface modification. In this work, a 1000 W MOPA pulsed fiber laser was used to strip a 75 +/- 5 mum thick iron-red epoxy primer from AH36 marine steel. Single-factor tests and an L9(3(3)) orthogonal design were evaluated using image-derived paint removal rate (PRR), surface roughness (Ra), SEM morphology, and energy-dispersive X-ray spectroscopy (EDS); every processing condition was repeated on five independently prepared coupons. Increasing pulse energy from 5.0 to 12.5 mJ raised the mean PRR from 79.01% to 99.67%, although the improvement above 7.5 mJ was marginal. At 70% overlap, PRR reached 99.12%, Fe was 77.7 wt.%, C was 8.1 wt.%, and Ra reached 4.196 mum, showing that high Fe exposure alone did not define the best surface state. Descriptive range values from the replicated L9 runs gave the apparent PRR order overlap rate > power > pulse duration; since the archived worksheet retained only run means, the small differences among the ranges cannot be tested against within-run error and are not treated as statistically significant. Run 2 (300 ns, 6.00 mJ at 100 kHz, and 60% overlap) was selected from the tested combinations as a multi-index engineering compromise rather than as a predicted optimum. It produced PRR = 99.90% and Ra = 1.308 mum. Pull-off testing used three dollies per recoated condition; the mean strength of the laser-cleaned condition was 5.20 MPa and failure was predominantly cohesive within the epoxy. FAU - Xing, Fayu AU - Xing F AD - Goertek College of Science and Technology Industry, Weifang University, Weifang 261061, China. FAU - Du, Xu AU - Du X AD - Yunnan Dianzhong Urban Construction Investment Development Co., Ltd., Kunming 650211, China. FAU - Xu, Fachen AU - Xu F AD - CNOOC Energy Development Equipment Technology Co., Ltd., Tianjin 300450, China. FAU - Li, Haoxiang AU - Li H AD - Merchant Marine College, Shanghai Maritime University, Shanghai 201306, China. FAU - Zhong, Ning AU - Zhong N AD - Merchant Marine College, Shanghai Maritime University, Shanghai 201306, China. FAU - Li, Yu AU - Li Y AD - Lumavia (Shanghai) Intelligent Technology Co., Ltd., Shanghai 201306, China. FAU - Meng, Liang AU - Meng L AD - Anhui Province Huaihe Vessel Inspection (Crew Management) Service Center, Bengbu 233000, China. FAU - Liu, Hongtao AU - Liu H AD - School of Machinery and Automation, Weifang University, Weifang 261061, China. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707100 OTO - NOTNLM OT - AH36 steel OT - MOPA laser cleaning OT - adhesion strength OT - parameter optimization OT - surface morphology COIS- Author Xu Du was employed by the company Yunnan Dianzhong Urban Construction Investment Development Co., Ltd. Author Fachen Xu was employed by the company CNOOC Energy Development Equipment Technology Co., Ltd. Author Yu Li was employed by the company Lumavia (Shanghai) Intelligent Technology Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:38 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ma19204338 [pii] AID - materials-19-04338 [pii] AID - 10.3390/ma19204338 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 15;19(20):4338. doi: 10.3390/ma19204338. PMID- 42941076 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 15 TI - An Accelerated Concrete Prism Test for Evaluating Fly Ash Mitigation of Alkali-Silica Reaction in Reactive Tuff Aggregates. LID - 10.3390/ma19204335 [doi] LID - 4335 AB - The concrete prism test (CPT) retains the aggregate size and specimen configuration of concrete and is widely used to evaluate measures for mitigating alkali-silica reaction (ASR). However, it requires a long test period, and stable control of moist-air curing conditions can be difficult. To improve the efficiency of concrete-scale ASR assessment, four reactive tuffs were investigated using the accelerated mortar bar test (AMBT), standard CPT, and an accelerated concrete prism test (ACPT) involving immersion in 1 mol/L NaOH at 80 degrees C. Grade I Class F fly ash was used at replacement levels of 0-35%, and the reaction characteristics of the tuff aggregates were examined by microscopy. With 15% fly ash, the 28 d AMBT expansions decreased from 0.212 to 0.376% to 0.036-0.050%, while the 728 d CPT expansions decreased from 0.037 to 0.064% to 0.018-0.029%. All values were below the corresponding expansion limits. The ACPT retained the concrete prism geometry and coarse aggregate grading while accelerating ASR under high-temperature alkaline exposure. The minimum fly ash replacements required to keep all tuff concretes below 0.040% were 20% at 182 d and 30% at 273 d. At 364 d, no replacement level up to 35% met this criterion for all mixtures. As the fly ash replacement increased from 0% to 15% and 35%, the visibly reacted depth of the tuff aggregate decreased from approximately 12 mm to 7 mm and 5 mm, respectively, consistent with the reduction in prism expansion. The results show that ACPT can distinguish the mitigation performance of different fly ash replacement levels within a specified test period. Its expansion criterion should therefore be linked to a fixed assessment age. The 182 d result may be used for rapid dosage selection, whereas the 273 d result provides a stricter recommendation for structures with higher durability and safety requirements. FAU - Liu, Xiaojun AU - Liu X AD - China General Nuclear Power Corporation, Shenzhen 518000, China. FAU - Zhang, Yan AU - Zhang Y AUID- ORCID: 0009-0003-3403-9042 AD - School of Architectural Engineering, Nanjing Institute of Technology, Nanjing 211167, China. AD - College of Materials Science and Engineering, Nanjing Tech University, Nanjing 211816, China. FAU - Lv, Guangye AU - Lv G AD - China General Nuclear Power Corporation, Shenzhen 518000, China. FAU - Li, Wei AU - Li W AD - College of Materials Science and Engineering, Nanjing Tech University, Nanjing 211816, China. FAU - Huang, Xiaojun AU - Huang X AD - College of Materials Science and Engineering, Nanjing Tech University, Nanjing 211816, China. FAU - Deng, Min AU - Deng M AD - College of Materials Science and Engineering, Nanjing Tech University, Nanjing 211816, China. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707283 OTO - NOTNLM OT - accelerated testing method OT - alkali-silica reaction OT - concrete prism test OT - fly ash OT - reactive tuff COIS- Authors Xiaojun Liu and Guangye Lv were employed by the company China General Nuclear Power corporation. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:38 PHST- 2026/08/16 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ma19204335 [pii] AID - materials-19-04335 [pii] AID - 10.3390/ma19204335 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 15;19(20):4335. doi: 10.3390/ma19204335. PMID- 42941073 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 15 TI - Experimental and Numerical Evaluation of Recycled Thermoplastic Composites for Structural Applications in Sustainable Construction. LID - 10.3390/ma19204331 [doi] LID - 4331 AB - This study presents an experimental and numerical framework for evaluating recycled thermoplastic composites for structural applications in sustainable construction. The base material (BM), comprising recycled high-density polyethylene (HDPE), low-density polyethylene (LDPE), and polypropylene (PP), was characterized in tension and compression; inverse mechanical identification yielded assigned domain fractions of 26%, 36%, and 38%, respectively. Numerical incorporation of acrylonitrile butadiene styrene (ABS) resulted in an optimized composite (CMo: 13% HDPE, 18% LDPE, 19% PP, and 50% ABS), increasing the predicted tensile elastic modulus from 871.36 MPa to 1670.4 MPa (91.7%). Thermal modeling illustrated the BM temperature distribution under prescribed boundary temperatures, while a preliminary structural assessment considered CMo beams, columns, and roof-support members for single-story housing and identified insufficient lateral stiffness under seismic loading. An inventory-based comparison yielded approximately 59% lower estimated CO(2)-equivalent emissions for the CMo structural system than for the reinforced-concrete and steel reference, subject to the adopted emission factors and differing source boundaries. The framework supports formulation selection for further investigation, although experimental validation of the CMo, members, and connections remains necessary before structural suitability can be established. FAU - Parada-Rocha, Karla AU - Parada-Rocha K AUID- ORCID: 0009-0008-2430-5235 AD - Department of Civil Engineering, Universidad Simon Bolivar, Barranquilla 080001, Colombia. FAU - Guzman, Andres AU - Guzman A AUID- ORCID: 0000-0003-2472-1390 AD - Department of Civil and Environmental Engineering, Universidad del Norte, Barranquilla 081007, Colombia. FAU - Parada-Rocha, Carlos AU - Parada-Rocha C AD - Department of Civil and Environmental Engineering, Universidad del Norte, Barranquilla 081007, Colombia. FAU - Daza-Marquez, Nemesio AU - Daza-Marquez N AUID- ORCID: 0000-0002-1159-6116 AD - Department of Civil and Environmental Engineering, Universidad del Norte, Barranquilla 081007, Colombia. LA - eng GR - Convocatoria 809 Formacion de Capital Humano de Alto Nivel para las Regiones/Colciencias/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707207 OTO - NOTNLM OT - agro-industrial waste OT - carbon footprint OT - finite element modeling OT - inverse identification OT - load-bearing members OT - low-rise housing OT - mechanical characterization OT - polymer blending COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:38 PHST- 2026/02/02 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ma19204331 [pii] AID - materials-19-04331 [pii] AID - 10.3390/ma19204331 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 15;19(20):4331. doi: 10.3390/ma19204331. PMID- 42941071 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 15 TI - Synthesis of "Yellow Cobblestones" of Sofia Using Asbestos Waste. LID - 10.3390/ma19204330 [doi] LID - 4330 AB - The "yellow cobblestones" of Sofia are considered one of the city's symbols. This high-quality clinker was developed in Budapest in the late 19th century by using a local marl. Since this paving was declared part of Sofia's cultural heritage, studies to synthesize similar materials were carried out. Unfortunately, the Bulgarian clays used are characterized by a lower MgO amount, which results in a very narrow sintering range. However, an increase in MgO in the parent batch, bringing the composition closer to that of the original, has a positive effect on sintering, phase composition and structure. This work discusses the possibility of obtaining similar clinker by adding asbestos waste containing a high percentage of MgO to a selected Bulgarian marl. The process involves pre-calcination of part of the marl together with the asbestos waste, followed by mixing the resulting grog with the remaining clay, homogenization, pressing and final firing. Studies with Hot Stage Microscopy and optical dilatometry demonstrate that the asbestos addition has a positive effect on the widening of the sintering interval, while XRD, SEM and pycnometric results elucidate that the samples obtained in this way are characterized by a phase composition and structure comparable to those of the original material. FAU - Karamanov, Alexander AU - Karamanov A AUID- ORCID: 0000-0003-4685-6544 AD - Institute for Physical Chemistry, Bulgarian Academy of Sciences, IPC-BAS, Bl. 11, Acad. G. Bonchev Str., 1113 Sofia, Bulgaria. FAU - Kostov-Kytin, Vladislav AU - Kostov-Kytin V AUID- ORCID: 0000-0002-8616-6698 AD - Institute of Mineralogy and Crystallography, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 107, 1113 Sofia, Bulgaria. FAU - Karamanova, Emilia AU - Karamanova E AD - Institute for Physical Chemistry, Bulgarian Academy of Sciences, IPC-BAS, Bl. 11, Acad. G. Bonchev Str., 1113 Sofia, Bulgaria. LA - eng GR - KP-06-N67/13/Bulgarian Science Fund/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707101 OTO - NOTNLM OT - asbestos OT - ceramics OT - cultural heritage OT - marl OT - sintering COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:38 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ma19204330 [pii] AID - materials-19-04330 [pii] AID - 10.3390/ma19204330 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 15;19(20):4330. doi: 10.3390/ma19204330. PMID- 42941069 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 15 TI - Effect of Build Orientation on the Electrical, Thermal, and Mechanical Properties of PA12 Fabricated by Selective Laser Sintering. LID - 10.3390/ma19204329 [doi] LID - 4329 AB - Selective laser sintering (SLS) provides a promising route for the rapid fabrication of polymeric insulating components with complex geometries. However, its layer-by-layer manufacturing process inherently introduces structural anisotropy, which may significantly affect the electrical, thermal, and mechanical properties of the printed materials. In this study, polyamide 12 (PA12) specimens fabricated by SLS at different printing orientations were systematically investigated for dielectric properties, volume resistivity, DC and AC breakdown strength, tensile properties, and thermal conductivity. Scanning electron microscopy and X-ray microscopy were further employed to characterize the microstructure and clarify the origin of the anisotropic behavior. The results show that SLS-printed PA12 exhibits pronounced orientation-dependent properties. As the printing angle increases from 0 degrees to 90 degrees , the volume resistivity and DC breakdown strength generally decrease. In contrast, the relative permittivity generally increases with printing angle, whereas the AC breakdown strength and dielectric loss exhibit more complex non-monotonic variations. The tensile strength and elongation at break both gradually decrease with increasing printing angle. The thermal conductivity varies only slightly across different orientations but increases overall with printing angle. Microstructural observations reveal numerous interlayer pores, incompletely sintered regions, and heterogeneous interfaces with a distinct layered distribution. These heterogeneous interlayer structures affect the transport of charge, heat, and load, thereby contributing to the anisotropic electrical, thermal, and mechanical properties of SLS-printed PA12. These findings provide a basis for optimizing the printing orientation and structural design of SLS-printed PA12 for high-voltage insulation applications. FAU - Huang, Le AU - Huang L AD - State Key Laboratory of Disaster Prevention and Reduction for Power Grid, Disaster Prevention and Reduction Center of State Grid Hunan Electric Power Co., Ltd., Changsha 410100, China. AD - School of Electrical Engineering and Automation, Wuhan University, Wuhan 430072, China. FAU - Tu, Sirui AU - Tu S AD - School of Electrical Engineering and Automation, Wuhan University, Wuhan 430072, China. FAU - Lyu, Yang AU - Lyu Y AUID- ORCID: 0009-0009-5021-5476 AD - State Key Laboratory of Disaster Prevention and Reduction for Power Grid, Disaster Prevention and Reduction Center of State Grid Hunan Electric Power Co., Ltd., Changsha 410100, China. FAU - Tu, Yanxin AU - Tu Y AUID- ORCID: 0000-0003-2542-5702 AD - School of Electrical Engineering and Automation, Wuhan University, Wuhan 430072, China. FAU - Li, Han AU - Li H AD - School of Electrical Engineering and Automation, Wuhan University, Wuhan 430072, China. LA - eng GR - SGHNFZ00SHJS2500079/State Key Laboratory of Disaster Prevention and Mitigation of Power Grid Transmission and Transformation Equipment/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13706837 OTO - NOTNLM OT - anisotropy OT - build orientation OT - electrical, thermal, and mechanical properties OT - high-voltage insulation OT - selective laser sintering COIS- Author Yang Lyu was employed by Disaster Prevention and Reduction Center, State Grid Hunan Electric Power Co., Ltd., Changsha, China. The funding sponsor had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. The remaining authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:38 PHST- 2026/08/25 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ma19204329 [pii] AID - materials-19-04329 [pii] AID - 10.3390/ma19204329 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 15;19(20):4329. doi: 10.3390/ma19204329. PMID- 42941051 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 13 TI - Use of Demineralized Autologous Dentin Graft with the Tooth Processing Device in Immediate Post-Extraction Implant Placement and Loading: A 12-Month Evaluation of Peri-Implant Soft Tissue Stability. LID - 10.3390/ma19204310 [doi] LID - 4310 AB - Purpose: To evaluate the effectiveness of immediate implant placement and loading combined with autologous dentin grafting in reducing post-extraction tissue remodelling, with particular emphasis on peri-implant soft tissue stability in the esthetic zone. Materials and Methods: This retrospective observational study included 13 patients (14 implant sites) with Type 1 extraction sockets in the anterior maxilla. Following atraumatic tooth extraction, implants were immediately placed and grafted with autologous dentin processed from the extracted tooth using the Tooth Transformer device. Screw-retained provisional restorations were immediately delivered through a digital workflow, and definitive restorations replicated the provisional transmucosal profile. Soft tissue dimensional changes were evaluated by superimposing stereolithography (STL) files from intraoral scans acquired before treatment and after at least 12 months. Vertical changes were measured as a single value, while horizontal changes were assessed 2 and 4 mm apical to the gingival margin. Results: All implants remained clinically stable, with no biological or prosthetic complications. Mean vertical soft tissue collapse was 0.69 mm, while mean horizontal collapse was 0.71 mm at 2 mm and 0.57 mm at 4 mm apical to the gingival margin. In most cases, dimensional changes were below 1 mm, with greater contour alterations at the more coronal level. Conclusions: Within the limitations of this study, immediate implant placement and loading combined with autologous dentin grafting using the Tooth Transformer device resulted in limited peri-implant soft tissue alterations after 12 months. This protocol may represent a biologically favourable, minimally invasive approach for ridge preservation while avoiding donor site morbidity and heterologous grafting materials. FAU - Bavetta, Giuseppe AU - Bavetta G AD - Private Practice, Studio Dentistico Associato Bavetta, 90143 Palermo, Italy. FAU - Di Carlo, Stefano AU - Di Carlo S AD - Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, Via Caserta 6, 00161 Rome, Italy. AD - Implanto-Prosthetic Unit, Policlinico Umberto I, Viale Regina Elena I 287b, 00161 Rome, Italy. FAU - Amelina, Giulia AU - Amelina G AUID- ORCID: 0009-0008-7061-6798 AD - Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, Via Caserta 6, 00161 Rome, Italy. AD - Implanto-Prosthetic Unit, Policlinico Umberto I, Viale Regina Elena I 287b, 00161 Rome, Italy. FAU - De Angelis, Francesca AU - De Angelis F AUID- ORCID: 0000-0002-7922-8870 AD - Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, Via Caserta 6, 00161 Rome, Italy. AD - Implanto-Prosthetic Unit, Policlinico Umberto I, Viale Regina Elena I 287b, 00161 Rome, Italy. FAU - Piccoli, Luca AU - Piccoli L AD - Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, Via Caserta 6, 00161 Rome, Italy. AD - Implanto-Prosthetic Unit, Policlinico Umberto I, Viale Regina Elena I 287b, 00161 Rome, Italy. FAU - Pranno, Nicola AU - Pranno N AUID- ORCID: 0000-0003-4095-0900 AD - Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, Via Caserta 6, 00161 Rome, Italy. AD - Implanto-Prosthetic Unit, Policlinico Umberto I, Viale Regina Elena I 287b, 00161 Rome, Italy. FAU - Guerrieri, Enrico AU - Guerrieri E AD - Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, Via Caserta 6, 00161 Rome, Italy. AD - Implanto-Prosthetic Unit, Policlinico Umberto I, Viale Regina Elena I 287b, 00161 Rome, Italy. FAU - Brauner, Edoardo AU - Brauner E AUID- ORCID: 0000-0001-7654-482X AD - Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, Via Caserta 6, 00161 Rome, Italy. AD - Implanto-Prosthetic Unit, Policlinico Umberto I, Viale Regina Elena I 287b, 00161 Rome, Italy. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707031 OTO - NOTNLM OT - autologous dentin graft OT - immediate implant placement OT - peri-implant soft tissue COIS- Author Giuseppe Bavetta is in private practice. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:38 PHST- 2026/08/24 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - ma19204310 [pii] AID - materials-19-04310 [pii] AID - 10.3390/ma19204310 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 13;19(20):4310. doi: 10.3390/ma19204310. PMID- 42941047 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 13 TI - Method for Automatic Detection of the Technical State of Insulated Coolboxes in the Cold Chain Based on IoT Sensor Data. LID - 10.3390/ma19204306 [doi] LID - 4306 AB - Maintaining temperature stability within the cold chain is critical to preventing food spoilage and financial loss. Insulated containers (coolboxes) using eutectic plates are widely employed to maintain passive cooling during transport. However, mechanical wear, seal damage, or improper latching can lead to microclimatic fluctuations inside the container, compromising product safety. This paper presents a non-invasive method for the automatic detection and classification of the technical state of coolboxes (fully functional, conditionally functional, and faulty) based on internal temperature and humidity measurements collected via wireless IoT sensors. Experimental trials conducted in a controlled climatic chamber simulating real-world operations demonstrate that micro-temperature gradients and heating rates allow for the early detection of seal degradation and incomplete door closures before critical thermal thresholds are violated. Specifically, the results confirm that uncompressed or damaged seals accelerate internal heating rates by up to 60%, and the diagnostic algorithm successfully classifies technical states with high sensitivity based on a degradation index threshold (∆Q 30% for faulty units). The proposed diagnostic framework supports predictive maintenance and improves overall cold-chain traceability. FAU - Lorenc, Augustyn AU - Lorenc A AUID- ORCID: 0000-0002-2109-2165 AD - Department of Transport and Logistics Systems (L-6), Faculty of Civil Engineering and Transport, PK Krakow University of Technology, 31-878 Krakow, Poland. FAU - Kuznar, Malgorzata AU - Kuznar M AUID- ORCID: 0000-0002-2876-4964 AD - Department of Rail Vehicles and Transport, Faculty of Mechanical Engineering, PK Krakow University of Technology, 31-864 Krakow, Poland. FAU - Pobedza, Janusz AU - Pobedza J AUID- ORCID: 0000-0001-7218-4935 AD - Laboratory of Techno-Climatic Research and Heavy Duty Machines, Faculty of Mechanical Engineering, PK Krakow University of Technology, 31-864 Krakow, Poland. FAU - Gawlik, Artur AU - Gawlik A AD - Laboratory of Techno-Climatic Research and Heavy Duty Machines, Faculty of Mechanical Engineering, PK Krakow University of Technology, 31-864 Krakow, Poland. FAU - Guzowski, Artur AU - Guzowski A AUID- ORCID: 0000-0002-9072-7515 AD - Laboratory of Techno-Climatic Research and Heavy Duty Machines, Faculty of Mechanical Engineering, PK Krakow University of Technology, 31-864 Krakow, Poland. LA - eng GR - POIR.01.01.01-00-0035/22/NCBR/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13706762 OTO - NOTNLM OT - IoT sensors OT - cold chain OT - coolbox OT - eutectic plates OT - food safety OT - insulated container OT - predictive maintenance OT - technical state diagnostics OT - thermal insulation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:38 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - ma19204306 [pii] AID - materials-19-04306 [pii] AID - 10.3390/ma19204306 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 13;19(20):4306. doi: 10.3390/ma19204306. PMID- 42941046 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 13 TI - Mechanical Investigation of CuZn30 Brass Under Conditions of Variable Axisymmetric Deformation. LID - 10.3390/ma19204305 [doi] LID - 4305 AB - This paper presents the results of investigations on CuZn30 brass subjected to cyclic oscillatory torsion. The authors hypothesized that by applying specific deformation conditions under which a 180 degrees change in the orientation of the principal components of the strain state occurs, it is possible to induce a change in the flow stress and strain. Experiments with various strain rates (0.01, 0.1 and 1 s(-1)) and different strain amplitudes (0.03, 0.06 and 0.3) were conducted at room temperature. Monotonic tests, carried out at the same temperature and under the same strain rates, served as reference results for the cyclic torsion tests. In metal forming processes, it is often assumed that elastic strains are so small compared to plastic strains that they can be neglected. However, during cyclic oscillatory torsion tests, elastic strain occurs in each deformation cycle and hinders the accurate determination of the true plastic strain. Therefore, the authors developed a dedicated method for separating elastic and plastic strains, which they applied to determine the plastic strains in individual cyclic hysteresis loops. In the cyclic torsion tests with the two smaller strain amplitudes (0.03 and 0.06), the increase in the limit strain was remarkably large-up to more than 80-fold. Such large strains were achieved at a flow stress significantly lower than that in the monotonic torsion tests, with a maximum stress drop of two and a half times. Conversely, in the tests conducted across all applied strain rates for the amplitude of 0.3, the stress was always distinctly higher, and the strain was much smaller than for the two smaller amplitudes. In all investigated cases, the results showed that for each applied strain rate, an increase in the strain amplitude leads to a very pronounced increase in the flow stress value and a reduction in the strain level, which is driven by the enhanced work-hardening of the material. The experimental results clearly demonstrate that precise control of cyclic strain parameters (strain rate and amplitude) allows for flexible tailoring of the flow stress and limit strain of the CuZn30 brass during cold forming. The authors demonstrated that forcing a 180 degrees change in the deformation direction can lower the yield strength and, consequently, improve the deformability of the CuZn30 brass. The obtained results may contribute to the development of energy-efficient, advanced metal forming processes, as an appropriately selected cyclic deformation scheme can significantly influence both the manufacturing process and the final properties of the product. FAU - Weiler, Wojciech AU - Weiler W AD - Metal Forming, Welding and Metrology Department, Wroclaw University of Science and Technology, ul. Ignacego Lukasiewicza 5, 50-371 Wroclaw, Poland. FAU - Jaskiewicz, Karol AU - Jaskiewicz K AD - Metal Forming, Welding and Metrology Department, Wroclaw University of Science and Technology, ul. Ignacego Lukasiewicza 5, 50-371 Wroclaw, Poland. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707181 OTO - NOTNLM OT - CuZn30 brass OT - cyclic torsion OT - deformation path OT - flow stress OT - limit strain OT - monotonic torsion OT - strain rate COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:38 PHST- 2026/08/06 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - ma19204305 [pii] AID - materials-19-04305 [pii] AID - 10.3390/ma19204305 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 13;19(20):4305. doi: 10.3390/ma19204305. PMID- 42941041 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 13 TI - Extraction of Various Magnesium Species from Slovenian Dolomites Using Chemical and Hydrometallurgical Approaches. LID - 10.3390/ma19204300 [doi] LID - 4300 AB - Although one of the most abundant elements, magnesium is classified as a critical raw material by the EU. Demand for Mg and its compounds is increasing because of their widespread applications and emerging potential uses in various industries. Most primary Mg metal production occurs outside the EU and is dominated by thermal reduction and molten salt electrolysis, both energy-intensive processes. Furthermore, converting dolomite, CaMg(CO(3))(2), one of the main Mg sources, into usable feed material requires high temperatures. In our study, the production of Mg(OH)(2) was achieved through a two-step process involving selective leaching of Slovenian dolomite with H(2)SO(4) and selective precipitation with hydroxide. The product contained 95% of the initial Mg and only 1% of Ca, resulting in a 97-fold enrichment in favour of Mg expressed as the Ca:Mg molar ratio. This process also supplied a CaSO(4) by-product, a potential source of Ca. Modifying the precipitation step with the use of chelating agents EDTA, EGTA, and DTPA did not significantly affect the Mg yield, but reduced Ca traces, thereby increasing the molar ratio to over 200. As an alternative pathway, calcination and silicothermic reduction of the dolomite demonstrated its suitability for the production of crystalline metallic Mg. FAU - Imperl, Jernej AU - Imperl J AUID- ORCID: 0009-0002-5472-5990 AD - Faculty of Chemistry and Chemical Technology, University of Ljubljana, Vecna Pot 113, 1000 Ljubljana, Slovenia. FAU - Zibret, Lea AU - Zibret L AUID- ORCID: 0000-0002-0132-0896 AD - Slovenian National Building and Civil Engineering Institute, Dimiceva Ulica 12, 1000 Ljubljana, Slovenia. FAU - Ducman, Vilma AU - Ducman V AUID- ORCID: 0000-0002-6430-3305 AD - Slovenian National Building and Civil Engineering Institute, Dimiceva Ulica 12, 1000 Ljubljana, Slovenia. FAU - Zibret, Gorazd AU - Zibret G AUID- ORCID: 0000-0002-9957-1895 AD - Geological Survey of Slovenia, Dimiceva Ulica 14, 1000 Ljubljana, Slovenia. FAU - Malensek Andolsek, Neza AU - Malensek Andolsek N AD - Geological Survey of Slovenia, Dimiceva Ulica 14, 1000 Ljubljana, Slovenia. FAU - Dankova, Zuzana AU - Dankova Z AUID- ORCID: 0000-0002-5089-2479 AD - State Geological Institute of Dionyz Stur, Regional Geological Service Kosice, Jesenskeho 8, 040 01 Kosice, Slovakia. FAU - Kollova, Zuzana AU - Kollova Z AUID- ORCID: 0009-0009-4045-8706 AD - State Geological Institute of Dionyz Stur, Regional Geological Service Kosice, Jesenskeho 8, 040 01 Kosice, Slovakia. FAU - Briancin, Jaroslav AU - Briancin J AD - Institute of Geotechnics, Slovak Academy of Sciences, Watsonova 45, 040 01 Kosice, Slovakia. FAU - Kolar, Mitja AU - Kolar M AUID- ORCID: 0000-0001-5095-6172 AD - Faculty of Chemistry and Chemical Technology, University of Ljubljana, Vecna Pot 113, 1000 Ljubljana, Slovenia. LA - eng GR - J1-50032/The Slovenian Research and Innovation Agency/ GR - P1-0153/The Slovenian Research and Innovation Agency/ GR - P2-0273/The Slovenian Research and Innovation Agency/ GR - P1-0025/The Slovenian Research and Innovation Agency/ GR - APVV-21-0176/Slovak Research and Development Agency/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13706612 OTO - NOTNLM OT - calcination OT - calcium sulphate OT - dolomite OT - magnesium OT - magnesium hydroxide OT - selective leaching OT - selective precipitation OT - thermal reduction COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:38 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - ma19204300 [pii] AID - materials-19-04300 [pii] AID - 10.3390/ma19204300 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 13;19(20):4300. doi: 10.3390/ma19204300. PMID- 42941037 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 12 TI - Preparation of Polyurethane Solid-Solid Phase-Change Material with Soft Segment for Application on Temperature-Regulating Fabrics. LID - 10.3390/ma19204296 [doi] LID - 4296 AB - The emergence of water-borne polyurethane solid-solid phase-change materials (PCMs) has overcome the problems of core material leakage in solid-liquid phase-change materials. However, water-borne polyurethane solid-solid phase-change materials also have issues such as poor heat storage performance and limited temperature regulation range. This study used polycaprolactone diol (PCL-diol) and poly(ethylene glycol) (PEG) as the composite soft segments, and selected dicyclohexylmethane-4,4'-diisocyanate (HMDI) and glycerol (GL) as the hard segments, to prepare a PEG-PCL-HMDI PCM. By adjusting the ratio of the composite soft and hard segments, the content of the soft segment, and the molar ratio of the monomers, the phase-change temperature was precisely controlled and the heat storage performance was synergistically improved. After the PEG-PCL-HMDI PCM had finished being applied onto the surface of cotton fabric, the temperature-regulating fabric exhibited a significant temperature-buffering effect in the 25-35 degrees C human activity temperature range, with the heating rate reduced and the cooling rate delayed. This study provides a new material system and process scheme for the development of intelligent textiles with both efficient energy storage and precise temperature regulation functions. FAU - Qiao, Xiran AU - Qiao X AUID- ORCID: 0000-0002-4610-2745 AD - School of Textile Science and Engineering, Xi'an Polytechnic University, No. 19 Jinhuanan Road, Xi'an 710048, China. AD - Key Laboratory of Advanced Dyeing and Finishing Technology of Shaanxi Higher Education Institutes, Xi'an Polytechnic University, Xi'an 710048, China. FAU - Lu, Shaofeng AU - Lu S AUID- ORCID: 0000-0002-6712-680X AD - School of Textile Science and Engineering, Xi'an Polytechnic University, No. 19 Jinhuanan Road, Xi'an 710048, China. AD - Key Laboratory of Advanced Dyeing and Finishing Technology of Shaanxi Higher Education Institutes, Xi'an Polytechnic University, Xi'an 710048, China. FAU - Yang, Qiancheng AU - Yang Q AD - School of Textile Science and Engineering, Xi'an Polytechnic University, No. 19 Jinhuanan Road, Xi'an 710048, China. AD - Key Laboratory of Advanced Dyeing and Finishing Technology of Shaanxi Higher Education Institutes, Xi'an Polytechnic University, Xi'an 710048, China. FAU - Lin, Songcong AU - Lin S AD - Fujian Techwork Textile Co., Ltd., Quanzhou 362700, China. FAU - Wang, Mingqiang AU - Wang M AD - School of Textile Science and Engineering, Xi'an Polytechnic University, No. 19 Jinhuanan Road, Xi'an 710048, China. AD - Key Laboratory of Advanced Dyeing and Finishing Technology of Shaanxi Higher Education Institutes, Xi'an Polytechnic University, Xi'an 710048, China. FAU - Shi, Wenzhao AU - Shi W AD - School of Textile Science and Engineering, Xi'an Polytechnic University, No. 19 Jinhuanan Road, Xi'an 710048, China. AD - Key Laboratory of Advanced Dyeing and Finishing Technology of Shaanxi Higher Education Institutes, Xi'an Polytechnic University, Xi'an 710048, China. FAU - Ma, Chao-Qun AU - Ma CQ AD - School of Textile Science and Engineering, Xi'an Polytechnic University, No. 19 Jinhuanan Road, Xi'an 710048, China. AD - Key Laboratory of Advanced Dyeing and Finishing Technology of Shaanxi Higher Education Institutes, Xi'an Polytechnic University, Xi'an 710048, China. LA - eng GR - 2024QCY-KXJ-118/Qin Chuangyuan's "Scientists & Engineers" Team Construction Project supported by the Shaanxi Provincial Government/ GR - 2025286/FuXiaQuan National Independent Innovation Demonstration Zone Project for Developing Strategic Emerging Industries and Future Industries/ GR - 24KGDW0036/Xi'an Scientists & Engineers Team Construction Project/ GR - 24JK0462/Scientific Research Program Funded by the Education Department of the Shaanxi Provincial Government/ GR - 25JP066/Scientific Research Program Funded by the Education Department of the Shaanxi Provincial Government/ GR - 2024CT006/Quanzhou City Introduction of High-level Talent Team Project/ GR - Research Start-up Fund Project of Xi'an Polytechnic University/ GR - 2024FTMP010 and 2025FTMP016/Ministry of Education/ GR - Xi'an Talent Program Youth Project - Innovative Talent/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707304 OTO - NOTNLM OT - composite soft segment OT - cotton fabric OT - solid-solid phase-change material OT - temperature-regulating fabric OT - water-borne polyurethane COIS- Author Songcong Lin was employed by the company Fujian Techwork Textile Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:38 PHST- 2026/07/16 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - ma19204296 [pii] AID - materials-19-04296 [pii] AID - 10.3390/ma19204296 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 12;19(20):4296. doi: 10.3390/ma19204296. PMID- 42941035 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 12 TI - Mix-Proportion Design and Properties of Steel Slag-Based Composite Cementitious Materials Using a Constrained Four-Component Mixture Design. LID - 10.3390/ma19204294 [doi] LID - 4294 AB - High-volume utilization of steel slag in cementitious materials requires a proportioning method that accounts for coupled effects among multiple components. This study investigated a four-component system comprising steel slag (SS), blast furnace slag (BFS), ordinary Portland cement (OPC), and desulfurized gypsum (DG), using 17 formulations within a constrained four-component mixture design. Unlike previous studies focused mainly on ternary binders or empirical proportioning, this work combined four-component formulation screening with age-dependent hydration characterization in one SS-BFS-OPC-DG framework. The 7 d compressive strength was selected as the modeled response because it provided a consistent early-age basis for comparing all 17 formulations within a practical testing period, whereas flexural strength was retained as a complementary experimental response. The compressive-strength mixture model was significant (p = 0.0044), with R(2) = 0.692, adjusted R(2) = 0.589, predicted R(2) = 0.435, and adequate precision = 8.545. Its performance was evaluated using prediction indices, residual diagnostics, and an independent confirmation batch. The selected mass fractions of SS, BFS, OPC, and DG were 0.322, 0.429, 0.131, and 0.116, respectively. The confirmation batch achieved a 7 d compressive strength of 24.80 MPa, compared with the predicted value of 24.93 MPa. The model was therefore used only for formulation screening and interpolation within the investigated component ranges. The selected formulation reached compressive and flexural strengths of 32.6 and 7.6 MPa at 28 d, respectively. XRD, SEM, TG, and FTIR results collectively supported continued formation of hydration products and progressive matrix densification from 3 d to 28 d. The C-S-H gel index increased from 2.07% to 6.15%, while the number of large visible voids decreased and the continuity of the matrix improved. These results provide a constrained mixture-design route for screening high-solid-waste SS-BFS-OPC-DG binders. FAU - Guo, Qiaoming AU - Guo Q AD - Jiangxi Provincial Communications Investment Group Co., Ltd., Nanchang 330108, China. FAU - Liu, Yuanbao AU - Liu Y AD - Jiangxi Provincial Communications Investment Group Co., Ltd., Nanchang 330108, China. FAU - Chen, Hui AU - Chen H AD - Jiangxi Provincial Communications Investment Group Co., Ltd., Nanchang 330108, China. FAU - Luo, Zhengbin AU - Luo Z AD - Jiangxi JPCI Maintenance Technology Group Co., Ltd., Nanchang 330108, China. FAU - Liu, Bin AU - Liu B AD - Jiangxi JPCI Maintenance Technology Group Co., Ltd., Nanchang 330108, China. FAU - Lin, Minghui AU - Lin M AD - School of Civil Engineering, Chongqing Jiaotong University, No. 66 Xuefu Road, Nan'an District, Chongqing 400074, China. FAU - Yang, Liu AU - Yang L AD - School of Civil Engineering, Chongqing Jiaotong University, No. 66 Xuefu Road, Nan'an District, Chongqing 400074, China. FAU - Chen, Zuming AU - Chen Z AD - School of Materials Science and Engineering, Chongqing Jiaotong University, No. 66 Xuefu Road, Nan'an District, Chongqing 400074, China. FAU - Zhao, Yi AU - Zhao Y AD - School of Materials Science and Engineering, Chongqing Jiaotong University, No. 66 Xuefu Road, Nan'an District, Chongqing 400074, China. LA - eng GR - 2024ZG020/the Science and Technology Project of Jiangxi Provincial Department of Transport/ GR - 20161BCF320025/the Key Research and Development Program of Jiangxi Province/ GR - JDLHPYJD2021011/the Joint Training Base Construction Project for Graduate Students in Chongqing/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707361 OTO - NOTNLM OT - cementitious materials OT - constrained mixture design OT - hydration characteristics OT - microstructure OT - mixture model COIS- Authors Qiaoming Guo, Yuanbao Liu and Hui Chen were employed by the Jiangxi Provincial Communications Investment Group Co., while authors Zhengbin Luo and Bin Liu were employed by the Jiangxi JPCI Maintenance Technology Group Co. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:38 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - ma19204294 [pii] AID - materials-19-04294 [pii] AID - 10.3390/ma19204294 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 12;19(20):4294. doi: 10.3390/ma19204294. PMID- 42941028 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 11 TI - The Influence of the Number and Position of the Methoxy Group on the Physicochemical Properties and Possible Applications of Rhodanine-3-Acetic Acid Derivatives. LID - 10.3390/ma19204287 [doi] LID - 4287 AB - In this study, we investigated the effect of the number and position of the methoxy group substitution on selected physicochemical and biological properties of rhodanine-3-acetic acid derivatives. We conducted our studies on a group of eight compounds (C-1-C-8) obtained by Knoevenagel condensation, with yields ranging from 49% to 99%. All synthesized compounds were thermally stable above 240 degrees C. Luminescence studies revealed the ICT nature of the absorption and emission spectra. Derivative C-4 exhibited the greatest blue shift, while compounds C-7 and C-8 exhibited the greatest red shift. Furthermore, our results indicate that compound C-2 is the best candidate from the entire group for reliable, artifact-free cytoplasmic staining. C-2 achieves excellent contrast in the FITC/Alexa488 channel while maintaining statistically unchanged 3T3 metabolic activity. In terms of antibacterial activity, the best results were obtained for the C-1 derivative, which demonstrated mild to moderate activity against Gram-positive bacteria. Compound C-8 demonstrated the highest anticancer activity against cancer cells such as SNB-19, Caco-2, and A2058. FAU - Szlapa-Kula, Agata AU - Szlapa-Kula A AUID- ORCID: 0000-0001-6539-5419 AD - Institute of Chemistry, Faculty of Science and Technology, University of Silesia in Katowice, Szkolna 9 St., 40-007 Katowice, Poland. FAU - Biernasiuk, Anna AU - Biernasiuk A AUID- ORCID: 0000-0002-2844-9876 AD - Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland. FAU - Kazmierski, Lukasz AU - Kazmierski L AUID- ORCID: 0000-0002-0861-8097 AD - Department of Urology and Andrology, Faculty of Medicine, Collegium Medicum, Nicolaus Copernicus University, Karlowicza 24, 85-092 Bydgoszcz, Poland. FAU - Kusmierz, Dariusz AU - Kusmierz D AD - Department of Cell Biology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Jednosci 9, 41-200 Sosnowiec, Poland. FAU - Plonka-Czerw, Justyna AU - Plonka-Czerw J AUID- ORCID: 0000-0003-0201-525X AD - Department of Cell Biology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Jednosci 9, 41-200 Sosnowiec, Poland. FAU - Latocha, Malgorzata AU - Latocha M AUID- ORCID: 0000-0003-4109-2629 AD - Department of Cell Biology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Jednosci 9, 41-200 Sosnowiec, Poland. FAU - Zegarska, Martyna AU - Zegarska M AD - Antoni Jurasz Univeristy Hospital No. 1 in Bydgoszcz, Marii Sklodowskiej-Curie 9, 85-094 Bydgoszcz, Poland. FAU - Kula, Slawomir AU - Kula S AUID- ORCID: 0000-0003-4520-1570 AD - Institute of Chemistry, Faculty of Science and Technology, University of Silesia in Katowice, Szkolna 9 St., 40-007 Katowice, Poland. FAU - Krawczyk, Przemyslaw AU - Krawczyk P AUID- ORCID: 0000-0002-3387-2820 AD - Department of Physical Chemistry, Faculty of Pharmacy, Collegium Medicum, Nicolaus Copernicus University, Kurpinskiego 5, 85-950 Bydgoszcz, Poland. LA - eng GR - No. BNW-1-105/K/4/F/Medical University of Silesia/ PT - Journal Article DEP - 20261011 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13706306 OTO - NOTNLM OT - antibacterial and antifungal activity OT - anticancer activity OT - bioimaging OT - fluorescent dyes OT - rhodanine-3-acetic acid derivatives COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/11 CRDT- 2026/10/27 10:38 PHST- 2026/08/09 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:38 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - ma19204287 [pii] AID - materials-19-04287 [pii] AID - 10.3390/ma19204287 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 11;19(20):4287. doi: 10.3390/ma19204287. PMID- 42941024 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 10 TI - Advances in Design and Preparation of Biodegradable Inferior Vena Cava Filters. LID - 10.3390/ma19204282 [doi] LID - 4282 AB - Venous thromboembolism (VTE) remains a leading cause of morbidity and mortality worldwide, with pulmonary embolism (PE) constituting its most fatal clinical consequence. Inferior vena cava filters (IVCFs) have been employed as a mechanical prophylactic strategy for patients with contraindications to anticoagulation or recurrent embolic events. However, permanent implantation and limited retrieval success are associated with device-related complications, including caval perforation, migration, fracture, and filter-induced thrombosis. Biodegradable IVCFs have been investigated as an alternative approach to provide temporary protection while avoiding the complications associated with long-term implantation. This review discusses recent advances in biodegradable IVCFs from a material perspective, focusing on material selection, structural design, degradation characteristics, and biological responses. Biodegradable polymers, degradable metallic materials, and shape-memory systems are evaluated regarding their mechanical stability, degradation behavior, vascular compatibility, and functional requirements. Strategies for improving radiopacity while preserving biodegradability are also summarized. Despite ongoing progress, key challenges remain in precisely controlling degradation kinetics, maintaining adequate mechanical support during the therapeutic period, and validating long-term safety through clinical studies. Further efforts in material optimization, biofunctional modification, and individualized fabrication may facilitate the future development of biodegradable IVCFs. FAU - Shen, Jiafan AU - Shen J AUID- ORCID: 0009-0007-7642-6373 AD - School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China. FAU - Li, Hailei AU - Li H AUID- ORCID: 0000-0003-2455-1782 AD - Department of Surgery, The University of Hong Kong, Hong Kong 999077, China. FAU - Ye, Lin AU - Ye L AUID- ORCID: 0000-0002-6568-3020 AD - School of Materials Science and Engineering, Beijing Institute of Technology, Beijing 100081, China. LA - eng GR - Z251100007125020/Beijing Association for Science and Technology/ PT - Journal Article PT - Review DEP - 20261010 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13707148 OTO - NOTNLM OT - biodegradable inferior vena cava filters OT - biodegradable polymers OT - degradation kinetics OT - structural design COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:37 PHST- 2026/08/08 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - ma19204282 [pii] AID - materials-19-04282 [pii] AID - 10.3390/ma19204282 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 10;19(20):4282. doi: 10.3390/ma19204282. PMID- 42941013 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 19 IP - 20 DP - 2026 Oct 9 TI - Metaheuristic-Driven Machine Learning for Best-Observed Feasible Weight Estimation of Braced Steel High-Rise Frames. LID - 10.3390/ma19204272 [doi] LID - 4272 AB - Preliminary selection of bracing systems in high-rise steel buildings requires comparing many configurations, each demanding computationally intensive minimum-weight design. This study uses machine learning to estimate the best-observed feasible weight directly from building-level configuration parameters. A full factorial dataset of 45 configurations covered three storey numbers (17, 24, and 30), three bracing types (V, X, and K), and five brace-placement layouts. Each configuration was optimized in a coupled SAP2000-MATLAB procedure with Particle Swarm, Grey Wolf, Honey Badger, and Aquila optimization (180 runs in total); no single algorithm was best for every configuration. Mean weight increased from 2537 kN at 17 storeys to 7103 kN at 30 storeys. X-bracing gave the highest mean weight at all heights and K-bracing the lowest at 17 and 24 storeys, whereas V- and K-bracing differed by only 2.4% at 30 storeys. Bracing type changed the mean weight by up to about 30%, and brace placement by about 10-15%. Among six regression models, Ridge achieved the highest mean five-fold cross-validated R(2) (0.914; 0.904 over 1000 repeated partitions), while Random Forest gave the lowest RMSE (511.7 kN); in leave-one-out prediction, Ridge ordered 90.3% of configuration pairs correctly. The resulting Ridge equation offers an interpretable screening tool within the investigated design domain. FAU - Ustuner, Betul AU - Ustuner B AUID- ORCID: 0000-0002-1936-3296 AD - Department of Civil Engineering, Faculty of Engineering and Natural Sciences, Manisa Celal Bayar University, Manisa 45030, Turkiye. FAU - Ozyuksel Ciftcioglu, Aybike AU - Ozyuksel Ciftcioglu A AUID- ORCID: 0000-0003-4424-7622 AD - Department of Civil Engineering, Faculty of Engineering and Natural Sciences, Manisa Celal Bayar University, Manisa 45030, Turkiye. FAU - Dogan, Erkan AU - Dogan E AUID- ORCID: 0000-0002-9691-2121 AD - Department of Civil Engineering, Faculty of Engineering and Natural Sciences, Manisa Celal Bayar University, Manisa 45030, Turkiye. FAU - Mardani, Ali AU - Mardani A AUID- ORCID: 0000-0003-0326-5015 AD - Department of Civil Engineering, Faculty of Engineering, Bursa Uludag University, Bursa 16059, Turkiye. LA - eng PT - Journal Article DEP - 20261009 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC13706733 OTO - NOTNLM OT - bracing systems OT - high-rise steel structures OT - machine learning OT - metaheuristic algorithms OT - structural optimization OT - weight prediction COIS- The authors declare no conflicts of interest. The funder had no role in the design, execution, interpretation, or writing of the study. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:37 PHST- 2026/09/08 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - ma19204272 [pii] AID - materials-19-04272 [pii] AID - 10.3390/ma19204272 [doi] PST - epublish SO - Materials (Basel). 2026 Oct 9;19(20):4272. doi: 10.3390/ma19204272. PMID- 42940997 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 21 TI - Gut Loading of Yellow Mealworm (Tenebrio molitor) Larvae with Dried Carrot: Effects on Retinol, Carotenoids, Tocopherols, Fatty Acid Profile, and Oxidative Status Biomarkers. LID - 10.3390/insects17101090 [doi] LID - 1090 AB - Gut loading may improve the nutritional value of feeder insects used for insectivorous animals. This study evaluated the effects of dried carrot inclusion in wheat-bran-based diets on the chemical composition, lipid-soluble bioactive compounds, fatty acid profile, and oxidative status of yellow mealworm (Tenebrio molitor) larvae. Larvae were assigned to three dietary treatments (10 replicates per treatment) and fed for 48 h with wheat bran alone (WB), 50% dried carrot and 50% wheat bran (DC50), or 75% dried carrot and 25% wheat bran (DC75). Dried carrot inclusion did not affect the proximate composition of the larvae. Retinol concentration was higher in DC75, whereas beta-carotene, lutein, and zeaxanthin increased with increasing dried carrot inclusion. In contrast, alpha-, beta-, and total tocopherol concentrations were lower in carrot-fed larvae. Dried carrot also modified the fatty acid profile, affecting several individual fatty acids as well as total polyunsaturated fatty acids (PUFA) and the n-6/n-3 PUFA ratio. Peroxidase and superoxide dismutase activities were unaffected, whereas malondialdehyde concentration was lower in DC75 than in WB. These results indicate that short-term feeding with dried carrot increases carotenoid concentrations in whole mealworm larvae and modifies selected indicators of lipid quality and oxidative status. FAU - Strychalski, Janusz AU - Strychalski J AUID- ORCID: 0000-0003-0948-3072 AD - Department of Fur-Bearing Animal Breeding and Game Management, University of Warmia and Mazury in Olsztyn, Oczapowskiego 2, 10-719 Olsztyn, Poland. FAU - Parchucik, Oskar AU - Parchucik O AD - Student Scientific Circle of Amateur Animal Breeders, University of Warmia and Mazury in Olsztyn, Oczapowskiego 2, 10-719 Olsztyn, Poland. FAU - Gugolek, Andrzej AU - Gugolek A AUID- ORCID: 0000-0002-5360-9755 AD - Department of Fur-Bearing Animal Breeding and Game Management, University of Warmia and Mazury in Olsztyn, Oczapowskiego 2, 10-719 Olsztyn, Poland. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705290 OTO - NOTNLM OT - dietary enrichment OT - feeder insects OT - insect nutrition OT - lipid peroxidation OT - nutrient composition OT - provitamin A OT - vitamin E COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:37 PHST- 2026/09/22 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - insects17101090 [pii] AID - insects-17-01090 [pii] AID - 10.3390/insects17101090 [doi] PST - epublish SO - Insects. 2026 Oct 21;17(10):1090. doi: 10.3390/insects17101090. PMID- 42940995 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 20 TI - Genetic Dynamics and Dispersion Pattern Changes in Aedes aegypti Populations (Diptera: Culicidae) in Yunnan Province, China, over Three Consecutive Years. LID - 10.3390/insects17101087 [doi] LID - 1087 AB - Aedes aegypti is the primary vector of mosquito-borne viruses and threatens human health globally. To monitor temporal changes in the population genetics of Ae. aegypti in Yunnan Province, we collected specimens from five sites between 2016 and 2018 and analyzed them at nine microsatellite loci and the mitochondrial cox I gene. Genetic diversity in these populations rose initially and then declined over the study period. The inbreeding coefficient shifted from a heterozygous state toward moderate inbreeding. Ten cox I haplotypes were identified, with haplotypes H06 and H09 prevalent across regions, while other haplotypes were limited to specific regions or years. Clustering analyses demonstrated significant population shifts of Ae. aegypti across the five sampling sites from 2016 to 2018. AMOVA analysis of all Ae. aegypti populations indicated that from 2016 to 2018, the main source of variation was predominantly among individuals, accounting for 76.43%, 65.15%, and 80.36%, respectively. Population dispersion patterns across locations initially increased and then decreased over time. Therefore, continuous monitoring is essential, and particular attention should be paid to intra-provincial spread, especially between port and urban areas. FAU - Gao, Jian AU - Gao J AD - Department of Disinfection and Vector Control, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China. AD - State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China. FAU - Zhang, Heng-Duan AU - Zhang HD AD - State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China. FAU - Wu, Zhi-Ming AU - Wu ZM AD - Department of Disinfection and Vector Control, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China. FAU - Shi, Qing-Ming AU - Shi QM AD - State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China. AD - Center for Disease Control and Prevention in Western Theater Command, Lanzhou 730030, China. FAU - Xing, Dan AU - Xing D AD - State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China. FAU - Guo, Xiao-Xia AU - Guo XX AD - State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China. FAU - Li, Chun-Xiao AU - Li CX AD - State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China. FAU - Dong, Yan-De AU - Dong YD AD - State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China. FAU - Zhao, Tong-Yan AU - Zhao TY AD - State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China. FAU - Chu, Hong-Liang AU - Chu HL AD - Department of Disinfection and Vector Control, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China. LA - eng GR - 2017ZX10303404/the Infective Disease Prevention and Cure Project of China/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705575 OTO - NOTNLM OT - Aedes aegypti OT - cox I OT - genetic structure OT - invasion OT - microsatellite OT - mosquitoes COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:37 PHST- 2026/08/19 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - insects17101087 [pii] AID - insects-17-01087 [pii] AID - 10.3390/insects17101087 [doi] PST - epublish SO - Insects. 2026 Oct 20;17(10):1087. doi: 10.3390/insects17101087. PMID- 42940993 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 20 TI - Host-Plant-Mediated Effects on the Demography, Parasitism Rates and Functional Response of Bracon hebetor on Spodoptera frugiperda. LID - 10.3390/insects17101085 [doi] LID - 1085 AB - Tritrophic interactions among host plants, herbivores and parasitoids can influence biological control efficacy, yet plant-mediated effects on parasitoid performance against the invasive fall armyworm, Spodoptera frugiperda, are poorly understood. We evaluated the effects of maize and castor on the life-history traits, demographic parameters, parasitism, functional response and population projection of the larval ectoparasitoid Bracon hebetor. Laboratory and greenhouse experiments used S. frugiperda larvae reared on the two host plants. Age-stage, two-sex life tables, parasitism indices, population projections and functional response models were used to compare parasitoid performance. Parasitoids developing on maize-fed larvae had significantly shorter developmental periods, higher fecundity (247.09 +/- 0.41 eggs), higher intrinsic (r = 0.217 +/- 0.008 day(-1)) and finite (lambda = 1.242 +/- 0.011) rates of increase and a shorter generation time (T = 20.26 +/- 0.289 days) than those on castor-fed larvae, whereas the net reproductive rate (R(0) = 81.54 +/- 11.55) and gross reproductive rate did not differ significantly. Effective parasitism and killing rates were higher on maize-fed larvae in both laboratory and greenhouse assays; non-effective parasitism did not differ significantly. Type II functional responses were observed on both host plants, and searching efficiency tended to be higher and handling time shorter on maize-fed larvae (Holling's disc equation: a = 1.125 vs. 0.787; T(h) = 0.0556 vs. 0.0817 days). Projections suggested faster parasitoid population growth and greater cumulative host killing on maize-fed larvae. These findings suggest that host-plant-mediated effects should be considered when optimizing sustainable biological control of S. frugiperda. FAU - Saw, Gouranga AU - Saw G AD - Biological Control Laboratory, Division of Entomology, Indian Council of Agricultural Research-Indian Agricultural Research Institute, New Delhi 110012, India. FAU - Jayanth, B V AU - Jayanth BV AUID- ORCID: 0000-0001-5383-4250 AD - Biological Control Laboratory, Division of Entomology, Indian Council of Agricultural Research-Indian Agricultural Research Institute, New Delhi 110012, India. FAU - Mahadev, Shashikala AU - Mahadev S AD - Biological Control Laboratory, Division of Entomology, Indian Council of Agricultural Research-Indian Agricultural Research Institute, New Delhi 110012, India. AD - Central Muga Eri Research and Training Institute, Lahdoigarh, Jorhat 785700, India. FAU - Rajareddy, Gundreddy AU - Rajareddy G AD - Biological Control Laboratory, Division of Entomology, Indian Council of Agricultural Research-Indian Agricultural Research Institute, New Delhi 110012, India. FAU - Udaykumar, Edula AU - Udaykumar E AD - Biological Control Laboratory, Division of Entomology, Indian Council of Agricultural Research-Indian Agricultural Research Institute, New Delhi 110012, India. FAU - Paul, Bishwajeet AU - Paul B AD - Biological Control Laboratory, Division of Entomology, Indian Council of Agricultural Research-Indian Agricultural Research Institute, New Delhi 110012, India. FAU - Jeer, Mallikarjuna AU - Jeer M AUID- ORCID: 0000-0003-0060-8414 AD - School of Crop Health Biology Research, ICAR-National Institute of Biotic Stress Management, Raipur 493225, India. FAU - Khan, Rashid Mumtaz AU - Khan RM AD - Department of Chemistry, College of Science, Qassim University, Buraydah 51452, Saudi Arabia. FAU - Gupta, Dhirendra Kumar AU - Gupta DK AUID- ORCID: 0009-0004-7636-4289 AD - Department of Statistics and Operations Research, College of Science, Qassim University, Buraidah 52571, Saudi Arabia. LA - eng GR - Qassim University/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705301 OTO - NOTNLM OT - biological control OT - demographic analysis OT - host plant effects OT - integrated pest management OT - parasitism rates OT - tritrophic interactions COIS- The authors declare that there are no conflicts of interest regarding the publication of this article. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:37 PHST- 2026/08/30 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - insects17101085 [pii] AID - insects-17-01085 [pii] AID - 10.3390/insects17101085 [doi] PST - epublish SO - Insects. 2026 Oct 20;17(10):1085. doi: 10.3390/insects17101085. PMID- 42940990 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 19 TI - Early Detection of Red Palm Weevil Infestation: A Systematic Review of Sensing Technologies, Artificial Intelligence, and Field Readiness. LID - 10.3390/insects17101081 [doi] LID - 1081 AB - The red palm weevil (Rhynchophorus ferrugineus Olivier; RPW) is an economically important invasive pest of palms, and early detection remains challenging because larval infestation develops internally before visible symptoms appear. This systematic review evaluates current RPW detection technologies, their operational limitations, and priorities for future development. Following PRISMA 2020, 56 primary studies were included, principally covering the period from 2015 to July 2026, together with selected seminal earlier studies. The evidence base was established through a systematic search across five bibliographic and scholarly sources, complemented by a structured supplementary update and source-verification procedure covering publications through 25 July 2026. Acoustic and vibroacoustic sensing provided the most direct evidence of active internal larval activity, whereas thermal, spectral, RGB, and UAV-based approaches primarily detected host physiological stress or externally observable damage. Although several studies reported high classification performance, most evidence was derived from private, single-site, proxy, or controlled datasets, with limited external field validation and inconsistent evaluation protocols. Multimodal and IoT-enabled systems showed potential for continuous and scalable monitoring, but consistent superiority over matched unimodal baselines was not established. No included study explicitly implemented and quantitatively evaluated a recognised explainable artificial intelligence method. The review also identifies major benchmarking and reproducibility limitations and proposes a conceptual six-layer weather-aware multimodal AI framework to guide the development of environmentally robust and field-deployable RPW monitoring systems. FAU - Gabralla, Lubna A AU - Gabralla LA AUID- ORCID: 0000-0002-6910-6861 AD - Department of Computer Science, Applied College, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi Arabia. FAU - Alshahrani, Amnah AU - Alshahrani A AUID- ORCID: 0000-0002-2239-9142 AD - Department of Information Systems, College of Computer and Information Sciences, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi Arabia. LA - eng GR - (RAP-2026-03)/Princess Nourah bint Abdulrahman University/ PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705317 OTO - NOTNLM OT - Internet of Things OT - acoustic sensing OT - artificial intelligence OT - early detection OT - explainable artificial intelligence OT - multimodal fusion OT - plant protection OT - red palm weevil OT - thermal imaging COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:37 PHST- 2026/08/17 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - insects17101081 [pii] AID - insects-17-01081 [pii] AID - 10.3390/insects17101081 [doi] PST - epublish SO - Insects. 2026 Oct 19;17(10):1081. doi: 10.3390/insects17101081. PMID- 42940985 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 17 TI - Broad-Complex Mediates 20-Hydroxyecdysone-Induced Antimicrobial Peptide Expression in the Silkworm Bombyx mori. LID - 10.3390/insects17101076 [doi] LID - 1076 AB - The steroid hormone 20-hydroxyecdysone (20E) is a key regulator of insect development and has recently emerged as an important modulator of innate immunity. However, the molecular mechanism by which 20E regulates antimicrobial peptide (AMP) expression in lepidopteran insects remains poorly understood. Here, we investigated the role of the 20E signaling pathway in antibacterial immunity in the silkworm Bombyx mori. Pretreatment with 20E significantly increased larval survival following infection with both Escherichia coli and Bacillus bombyseptieus and accelerated bacterial clearance in vivo. Consistently, 20E rapidly induced the expression of multiple AMP genes in the fat body in a dose- and time-dependent manner. Promoter analysis revealed that Broad-Complex (BrC) binding motifs were highly enriched in most AMP promoters. Functional analyses demonstrated that BrC-Z isoforms selectively activated different AMP promoters, with Leb3 showing the strongest response. Site-directed mutagenesis and electrophoretic mobility shift assays confirmed that BrC-Z directly binds to the Leb3 promoter to activate transcription. We further identified a previously uncharacterized splice variant, BrC-N, which lacks the zinc-finger DNA-binding domain and cannot activate transcription independently but markedly enhances BrC-Z-dependent Leb3 activation, likely through BTB/POZ-mediated protein interactions. Together, these findings establish a 20E-BrC-AMP regulatory axis underlying silkworm antibacterial immunity and reveal a previously unknown mechanism by which a noncanonical BrC isoform potentiates hormone-dependent immune gene expression. FAU - Xian, Pengjie AU - Xian P AD - Institute of Basic Medicine, North Sichuan Medical College, Nanchong 637100, China. FAU - Deng, Tingting AU - Deng T AD - Institute of Basic Medicine, North Sichuan Medical College, Nanchong 637100, China. FAU - Li, Xiaoqin AU - Li X AD - Institute of Basic Medicine, North Sichuan Medical College, Nanchong 637100, China. FAU - Zhang, Yuxin AU - Zhang Y AD - Institute of Basic Medicine, North Sichuan Medical College, Nanchong 637100, China. FAU - Guo, Xin AU - Guo X AD - Department of Chemistry, School of Pharmacy and Institute of Pharmacy, North Sichuan Medical College, Nanchong 637100, China. FAU - Wang, Liang AU - Wang L AD - Institute of Basic Medicine, North Sichuan Medical College, Nanchong 637100, China. FAU - He, Xiu AU - He X AD - Institute of Basic Medicine, North Sichuan Medical College, Nanchong 637100, China. LA - eng GR - 82601083/National Natural Science Foundation of China/ GR - 2026YFTX0046/Science and Technology Department of Sichuan Province/ GR - CBY22-QDA16/Doctoral Initiation Fund of North Sichuan Medical College/ GR - 24QNMP055/Health Commission of Sichuan Province Medical Science and Technology Program/ GR - 26MSZX579/Science and Technology Research Special Program of Sichuan Provincial Administration of Traditional Chinese Medicine/ GR - 2023PTZK004/Research and Development Program of the Affiliated Hospital of North Sichuan Medical College/ PT - Journal Article DEP - 20261017 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705130 OTO - NOTNLM OT - AMP OT - broad-complex OT - ecdysteroid OT - immune COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:37 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - insects17101076 [pii] AID - insects-17-01076 [pii] AID - 10.3390/insects17101076 [doi] PST - epublish SO - Insects. 2026 Oct 17;17(10):1076. doi: 10.3390/insects17101076. PMID- 42940984 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 17 TI - Isolation and Evaluation of the Gut-Associated Bacterium Staphylococcus gallinarum KI4: Effects on Survival and Cocoon Traits of Bombyx mori Under Bacillus cereus Challenge. LID - 10.3390/insects17101075 [doi] LID - 1075 AB - Indigenous insect-associated bacteria may provide biological resources for improving disease resilience in insect production. We isolated and functionally screened gut-associated bacteria from the Thai polyvoltine Nang Lai strain of Bombyx mori. Fifty colonies were recovered following alkaline enrichment. Partial 16S rRNA gene sequence analysis and phylogenetic comparison assigned isolates KI1, KI2, KI3, KI4, and KI9 to Staphylococcus gallinarum and KI6 to Enterococcus mundtii; the sequences were deposited in GenBank under accession numbers PZ319142-PZ319147. KI2 and KI4 produced inhibition zones against Bacillus cereus, Bacillus thuringiensis, and Serratia marcescens, whereas KI6 and KI9 showed no detectable inhibition. KI4 was selected for in vivo evaluation because it produced the largest inhibition zone against B. cereus (18.0 +/- 1.1 mm). Under non-challenged conditions, KI4 supplementation did not differ significantly from the untreated control in final larval weight, cocooning, pupation, fresh cocoon weight, or shell weight. B. cereus challenge reduced endpoint survival to 49.33 +/- 2.31%, whereas daily KI4 supplementation initiated before challenge and continued thereafter was associated with higher endpoint survival (77.33 +/- 2.31%) and higher cocooning and pupation than the pathogen-only treatment. The in vitro antagonism and in vivo host response represent separate observations because a non-antagonistic bacterial control was not included. These findings support further evaluation of S. gallinarum KI4 (GenBank accession no. PZ319145) as a candidate host-derived bacterial resource for sericulture. Intestinal persistence, the mechanism and specificity of the host response, biosafety, and field efficacy require further investigation before practical application. FAU - Terenius, Olle AU - Terenius O AUID- ORCID: 0000-0002-9909-1859 AD - Department of Cell and Molecular Biology, Uppsala University, 752 37 Uppsala, Sweden. FAU - Suraporn, Siripuk AU - Suraporn S AD - Department of Biology, Faculty of Science, Mahasarakham University, Kantarawichai District, Maha Sarakham 44150, Thailand. LA - eng GR - Thailand Science Research and Innovation/ PT - Journal Article DEP - 20261017 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705185 OTO - NOTNLM OT - Bacillus cereus OT - Bombyx mori OT - Staphylococcus gallinarum OT - bacterial antagonism OT - gut-associated bacteria OT - host protection OT - resource insect OT - sericulture COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:37 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - insects17101075 [pii] AID - insects-17-01075 [pii] AID - 10.3390/insects17101075 [doi] PST - epublish SO - Insects. 2026 Oct 17;17(10):1075. doi: 10.3390/insects17101075. PMID- 42940983 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 17 TI - Comparative Analysis of Thrips Prey and Pollen Usage by Three Predatory Mites, Amblyseius largoensis, Neoseiulus californicus and Neoseiulus barkeri. LID - 10.3390/insects17101074 [doi] LID - 1074 AB - Phytoseiid mites (PDMs) utilize both herbivorous prey and an alternative food source in agroecosystems. However, biocontrol screening of PDMs has largely focused on their predation function against prey, with their use of alternative food often ignored. Therefore, in the present study, we compared the performance of three PDM species (Amblyseius largoensis (Muma), Neoseiulus californicus (McGregor) and Neoseiulus barkeri Hughes), fed on two food sources: onion thrips (Thrips tabaci) and pollen (an alternative food) on potato plants. All three PDMs concentrated their attacks on the vulnerable prey stages, with early larval instars attacked at the highest rate, while N. californicus proved the most effective egg predator and N. barkeri the least. A. largoensis located and attacked prey the fastest both in leaf arenas and on intact plants, despite the longest per-prey handling time and attacked the fewest prey across a density gradient while leaving the least thrips-induced leaf damage. Prey rather than pollen was the primary driver of survival in every PDM, and A. largoensis persisted longest under food limitation and was the only species to gain further benefit from pollen when prey was already available. This ranking inverted in mixed-species arenas, where N. californicus consistently outsurvived the other two species under prey-present and pollen-only conditions. Foraging efficiency, food resource use and intraguild dominance were dissociated for PDM performance. Thus, a multiple-trait screening can be considered in PDM selection for thrips management in crops. FAU - Munawar, Asim AU - Munawar A AUID- ORCID: 0000-0002-9101-9545 AD - State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. FAU - Kaiyu, Li AU - Kaiyu L AD - State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. FAU - Xuan, Chen AU - Xuan C AD - State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. FAU - Abou El-Ela, Amr S AU - Abou El-Ela AS AUID- ORCID: 0000-0003-2195-4498 AD - State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. AD - Plant Protection Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Egypt. FAU - Zhu, Zengrong AU - Zhu Z AUID- ORCID: 0000-0002-3247-1486 AD - State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. FAU - Fang, Xiaoduan AU - Fang X AUID- ORCID: 0000-0001-8286-4432 AD - Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Institute of Zoology, Guangdong Academy of Sciences, Guangzhou 510260, China. FAU - Wang, Zhizhi AU - Wang Z AUID- ORCID: 0000-0003-0189-1741 AD - State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. FAU - Fu, Jianwei AU - Fu J AD - Institute of Crop Sciences, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China. AD - Institute of Quality Standards and Testing Technology for Agro-Products, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China. FAU - Xu, Ruibin AU - Xu R AD - State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. AD - Institute of Quality Standards and Testing Technology for Agro-Products, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China. AD - College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Zhou, Wenwu AU - Zhou W AD - State Key Laboratory of Rice Biology and Breeding, Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. LA - eng GR - 2023YFC2604500/National Key R&D Program/ GR - W2433069, 32272636, 32572935/National Natural Science Foundation of China/ GR - CARS-21-C05/earmarked fund for China Agriculture Research System/ PT - Journal Article DEP - 20261017 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705122 OTO - NOTNLM OT - Phytoseiidae OT - Thrips tabaci OT - alternative food OT - augmentative biological control OT - intraguild competition OT - prey-stage selectivity COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:37 PHST- 2026/09/15 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - insects17101074 [pii] AID - insects-17-01074 [pii] AID - 10.3390/insects17101074 [doi] PST - epublish SO - Insects. 2026 Oct 17;17(10):1074. doi: 10.3390/insects17101074. PMID- 42940980 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 16 TI - Identification and Behavioral Evaluation of Ketone Attractants for the Stable Fly, Stomoxys calcitrans. LID - 10.3390/insects17101071 [doi] LID - 1071 AB - The stable fly, Stomoxys calcitrans L., is one of the most damaging blood-feeding pests of livestock. Stable flies use odors associated with vertebrate hosts and their environments to locate blood meal and suitable oviposition and larval-development sites. Previous studies have identified behaviorally active gases, phenolic compounds, short-chain fatty acids, alcohols, and sulfur-containing compounds from cattle-associated odor sources, but ketones have received limited behavioral investigation. We first evaluated stable-fly attraction to host- and resource-associated substrates, including chicken manure, wet blood meal and sheep blood and characterized volatile compounds from attractive materials using SPME-GC-MS. We then tested ketones detected in the headspace of these substrates or reported in published studies. Three ketones, 1-octen-3-one, 3-octanone, and 6-methyl-5-hepten-2-one, significantly attracted adult stable flies. Following blend evaluation, 3-octanone and 1-octen-3-one were selected as the two-component K-lure blend which was significantly more attractive than the control in choice assays. Combining K-lure with the previously reported phenol-m-cresol blend elicited the highest response among the treatments tested. In a subsequent sticky-card trapping assay, traps baited with K-lure captured significantly more stable flies than the control traps. These results identify ketones as stable fly attractants and support further evaluation of K-lure in trap-based monitoring and management. FAU - Parepely, Saravan Kumar AU - Parepely SK AD - Apex Bait Technologies, Inc., Santa Clara, CA 95054, USA. FAU - Maurya, Abhinav Kumar AU - Maurya AK AD - Apex Bait Technologies, Inc., Santa Clara, CA 95054, USA. FAU - Liang, Dangsheng AU - Liang D AD - Apex Bait Technologies, Inc., Santa Clara, CA 95054, USA. LA - eng GR - 2023-40000-40953/United States Department of Agriculture/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705007 OTO - NOTNLM OT - Stomoxys calcitrans OT - attractant OT - ketones OT - olfactory lure OT - semiochemicals OT - stable fly COIS- Authors Saravan Kumar Parepely, Abhinav Kumar Maurya, and Dangsheng Liang were employed by Apex Bait Technologies, Inc. during the conduct of this research. The research described in this manuscript concerns semiochemical attractant technology with potential commercial application by Apex Bait Technologies, Inc. Apex Bait Technologies, Inc. plans to file a patent application related to the attractant technology described in this manuscript. The authors declare no other competing financial or non-financial interests. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:37 PHST- 2026/08/25 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - insects17101071 [pii] AID - insects-17-01071 [pii] AID - 10.3390/insects17101071 [doi] PST - epublish SO - Insects. 2026 Oct 16;17(10):1071. doi: 10.3390/insects17101071. PMID- 42940974 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 15 TI - Genetic and Biological Basis of High Royal Jelly-Producing Bees Apis mellifera ligustica: From Larval Signal Perception to Glandular Secretion. LID - 10.3390/insects17101065 [doi] LID - 1065 AB - Royal jelly (RJ), the glandular secretion that determines honeybee caste, is also a high-value apicultural product. China supplies about 90% of the world production volume, almost entirely through high RJ-producing bees (RJBs) selected from Apis mellifera ligustica over four decades, or Chinese-selected a. m. ligustica population; these yield up to ten times more RJ per colony than ITBs. Here, we review and assemble the morphological, proteomic, genomic and behavioural evidence for that unusual selection response at four levels. RJ production may be dominated by a heritable polygenic trait whose acceptance and secretion components carry significant genotypic variance, and the selected candidate genomic architecture combines a large inversion with the tandem mrjp cluster. RJB nurses could detect larval volatiles more sensitively, and cross-fostering suggests that larval acceptance depends on the genotype of the nurse bees, not that of the larvae. RJB hypopharyngeal glands develop larger acini, delayed regression and reinforced protein synthesis, whereas mandibular glands up-regulate the fatty-acid pathway that may yield 10-HDA. An expanded nurse-stage neuropeptidome and a remodelled brain membrane proteome may tune larval recognition, water homeostasis and pollen collection. The proposed three coupled feedback loops and outline the experiments needed to test the model. FAU - Wu, Na AU - Wu N AD - Beijing Houdeli Bee-Products Company, East Dajiaoting Middle Street, Beijing 100124, China. FAU - Ao, Weiran AU - Ao W AD - Beijing Houdeli Bee-Products Company, East Dajiaoting Middle Street, Beijing 100124, China. FAU - Li, Zhiping AU - Li Z AD - Beijing Houdeli Bee-Products Company, East Dajiaoting Middle Street, Beijing 100124, China. FAU - Li, Jianke AU - Li J AD - Beijing Houdeli Bee-Products Company, East Dajiaoting Middle Street, Beijing 100124, China. AD - Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Yumingyuanxi Road, Beijing 100191, China. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705297 OTO - NOTNLM OT - 10-HDA OT - Apis mellifera ligustica OT - artificial selection OT - hypopharyngeal gland OT - larval pheromone OT - neuropeptidome OT - olfaction OT - royal jelly COIS- Author Na Wu, Weiran Ao, Zhiping Li are employed by Beijing Houdeli Bee-products Company. The remaining authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:37 PHST- 2026/09/21 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - insects17101065 [pii] AID - insects-17-01065 [pii] AID - 10.3390/insects17101065 [doi] PST - epublish SO - Insects. 2026 Oct 15;17(10):1065. doi: 10.3390/insects17101065. PMID- 42940969 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 14 TI - Functional Response and Predation Potential of Arma custos (Fabricius) Against Tuta absoluta (Meyrick). LID - 10.3390/insects17101060 [doi] LID - 1060 AB - The tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), is a globally invasive pest causing severe economic losses in tomato production. We evaluated the predatory stink bug Arma custos (Fabricius) (Hemiptera: Pentatomidae) using functional-response assays, a predator-density experiment, behavioral observations, Y-tube olfactometer bioassays, and a greenhouse cage trial. Depletion-aware maximum-likelihood models supported a Type III response for adult females feeding on second-instar larvae, whereas Type II models were retained for fifth-instar nymphs and adults feeding on fourth-instar larvae. At an initial density of 40 larvae, females consumed 16.2 +/- 0.2 second-instar or 17.4 +/- 0.5 fourth-instar larvae within 24 h. With 40 fourth-instar larvae available per arena, increasing female density from one to five reduced per capita consumption from 17.40 +/- 0.51 to 5.44 +/- 0.04 larvae per day. The Hassell-Varley model provided the best fit (m = 0.725; 95% CI, 0.693-0.755). In greenhouse cages, releasing three females per cage reduced cumulative damaged leaves by 60.12% by day 15. No active larvae were detected in release cages at that time, compared with 11.33 +/- 0.33 in controls. Females preferred infested over healthy tomato plants, whereas males showed no significant preference. These findings demonstrate that A. custos is a promising biological control agent for integrated pest management (IPM) of T. absoluta. FAU - Jiang, Yuyang AU - Jiang Y AD - State Key Laboratory of Agricultural and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Zhao, Yan AU - Zhao Y AUID- ORCID: 0000-0002-5266-1252 AD - State Key Laboratory of Agricultural and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Li, Xiujie AU - Li X AD - State Key Laboratory of Agricultural and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Peng, Linli AU - Peng L AD - State Key Laboratory of Agricultural and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Wu, Sheng-Yen AU - Wu SY AUID- ORCID: 0000-0002-1795-3030 AD - State Key Laboratory of Agricultural and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Hou, Youming AU - Hou Y AUID- ORCID: 0000-0001-7984-9688 AD - State Key Laboratory of Agricultural and Forestry Biosecurity, Fujian Agriculture and Forestry University, Fuzhou 350002, China. LA - eng GR - 32361143791/National Natural Science Foundation of China/ GR - U22A20489/National Natural Science Foundation of China/ GR - 2025I0009/International Cooperation Project of Fujian Province/ GR - 2024J01377/Fujian Academy of Environmental Sciences/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705228 OTO - NOTNLM OT - greenhouse cage trial OT - intraspecific interference OT - olfactory preference OT - predatory functional traits OT - the predatory stink bug OT - the tomato leafminer COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:37 PHST- 2026/08/04 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - insects17101060 [pii] AID - insects-17-01060 [pii] AID - 10.3390/insects17101060 [doi] PST - epublish SO - Insects. 2026 Oct 14;17(10):1060. doi: 10.3390/insects17101060. PMID- 42940968 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 14 TI - Odorant Receptors Mediating Benzaldehyde Detection in Mythimna separata: Functional Identification and Ligand Selectivity. LID - 10.3390/insects17101059 [doi] LID - 1059 AB - Benzaldehyde (BA) is a ubiquitous aromatic volatile produced by both plants and insects. In Mythimna separata, it also serves as a male-emitted pheromonal signal that mediates male-male competition by antagonizing the pheromone receptor MsepPR3 and disrupting the perception of the female sex pheromone Z11-16:Ald. Whether M. separata also possesses conventional odorant receptors (ORs) that detect BA as an environmental volatile remains unknown. Here, we investigated the peripheral molecular basis of BA detection in M. separata. Electroantennogram (EAG) recordings revealed robust, dose-dependent responses to BA in both female and male adults, demonstrating that the antennal olfactory system readily detects BA. Candidate ORs were selected based on phylogenetic relationships and functional evidence from homologous receptors in other noctuid moths and subsequently characterized using the Xenopus oocyte expression system. Two BA-responsive ORs with distinct ligand profiles were identified. MsepOR42 displayed a highly selective response to BA, whereas MsepOR29 showed broader tuning and responded to BA, benzyl alcohol and 2-phenylethanol. These results establish the peripheral molecular basis of BA detection in M. separata and reveal that this ecologically important volatile is encoded by ORs with contrasting ligand selectivity. Our findings expand the understanding of aromatic volatile coding in moths and identify candidate molecular targets for odor-based pest management. FAU - Sun, Dongdong AU - Sun D AUID- ORCID: 0009-0007-2116-3983 AD - Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Synthetic Biology Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China. AD - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. FAU - Wang, Xiaoqing AU - Wang X AD - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. FAU - Fan, Yiping AU - Fan Y AD - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. FAU - Zhang, Bo AU - Zhang B AD - Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Synthetic Biology Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China. FAU - Yu, Wenyi AU - Yu W AD - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. FAU - Liu, Yang AU - Liu Y AUID- ORCID: 0000-0002-3424-7111 AD - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. FAU - Wang, Guirong AU - Wang G AD - Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Synthetic Biology Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China. AD - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. LA - eng GR - 32130089 and 32572938/National Natural Science Foundation of China/ GR - CAAS-CSCB-202302/Agricultural Science and Technology Innovation Program/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705561 OTO - NOTNLM OT - Mythimna separata OT - benzaldehyde OT - insect olfaction OT - ligand selectivity OT - odorant receptor COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:37 PHST- 2026/08/29 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - insects17101059 [pii] AID - insects-17-01059 [pii] AID - 10.3390/insects17101059 [doi] PST - epublish SO - Insects. 2026 Oct 14;17(10):1059. doi: 10.3390/insects17101059. PMID- 42940957 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 12 TI - Repeat-Rich Architecture and Gene Duplication Characterize the Expanded Genome of the Chestnut Gall Wasp. LID - 10.3390/insects17101047 [doi] LID - 1047 AB - Dryocosmus kuriphilus, the chestnut gall wasp, is a globally invasive pest that reproduces by thelytokous parthenogenesis. Despite the absence of conventional sexual recombination, the species has spread widely and persists under diverse environmental conditions, but the genomic features associated with this capacity remain poorly understood. Here, a chromosome-level assembly of D. kuriphilus was compared with seven representative insect genomes, with emphasis on repetitive sequences, orthogroups, and gene-duplication patterns. The 2.28-Gb D. kuriphilus genome contained 79.7% repetitive sequences, including 62.3% transposable elements (TEs), most of which occurred in intergenic regions. OrthoFinder assigned 13,271 D. kuriphilus genes to multi-copy orthogroups, representing 61.5% of the protein-coding gene set. Independent MCScanX analysis classified genes into mutually exclusive dispersed, proximal, tandem, and segmental duplication modes, with segmental and dispersed representing the two largest duplicated-gene classes. D. kuriphilus contained substantially more segmental duplicates than the other species examined. Synonymous-substitution distributions revealed low- and high-Ks components representing different levels of synonymous divergence among duplicated genes, and genes in these components showed distinct functional profiles. Numerous local duplications were found near TEs and included genes involved in host recognition, nutrient utilisation, xenobiotic metabolism, and energy production. Compared with the closely related gall wasp Belonocnema kinseyi, D. kuriphilus has a broader reported distribution and host range and contains higher copy numbers in several gene families associated with chemosensation, detoxification, metabolism, and stress responses. Together, these findings show that the expanded D. kuriphilus genome is characterized by extensive repeat accumulation and increased gene copy number, whereas duplication at multiple scales increased and reshaped the functional gene repertoire. These genomic features identify candidate mechanisms that may contribute to genomic and ecological plasticity, although their adaptive significance requires expression, evolutionary, and functional validation. FAU - Ren, Ye-Song AU - Ren YS AD - Laboratory of Insect Behavior and Evolutionary Ecology, College of Life Sciences, Central South University of Forestry and Technology, Changsha 410004, China. FAU - Lv, An-Kang AU - Lv AK AUID- ORCID: 0000-0001-8063-6153 AD - Laboratory of Insect Behavior and Evolutionary Ecology, College of Life Sciences, Central South University of Forestry and Technology, Changsha 410004, China. FAU - Zeng, Yang AU - Zeng Y AD - Laboratory of Insect Behavior and Evolutionary Ecology, College of Life Sciences, Central South University of Forestry and Technology, Changsha 410004, China. FAU - Zhu, Dao-Hong AU - Zhu DH AUID- ORCID: 0000-0003-2442-672X AD - Laboratory of Insect Behavior and Evolutionary Ecology, College of Life Sciences, Central South University of Forestry and Technology, Changsha 410004, China. LA - eng GR - 2026JJ90219/Department of Science and Technology of Hunan Province/ GR - Grant NO. CX20251234/Hunan Provincial Innovation Foundation For Postgraduate/ GR - Grant NO. 2025CX01090/Scientific Innovation Fund for Post-graduates of Central South University of Forestry and Technology/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13704927 OTO - NOTNLM OT - Dryocosmus kuriphilus OT - environmental adaptation OT - gene duplication OT - genome expansion OT - parthenogenesis OT - transposable elements COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:37 PHST- 2026/08/17 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - insects17101047 [pii] AID - insects-17-01047 [pii] AID - 10.3390/insects17101047 [doi] PST - epublish SO - Insects. 2026 Oct 12;17(10):1047. doi: 10.3390/insects17101047. PMID- 42940952 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 10 TI - Host Pre-Adaptation and Sublethal Indoxacarb Interactively Affect Growth and Development of Cnaphalocrocis medinalis, with Potential Involvement of P450 and Juvenile Hormone-Related Genes. LID - 10.3390/insects17101043 [doi] LID - 1043 AB - Host switching alters insecticide responses, but its interplay with sublethal stress remains unclear. We examined if host pre-adaptation modulates sublethal indoxacarb effects on Cnaphalocrocis medinalis and whether P450 and juvenile hormone-related genes are involved. Two long-term laboratory populations with different origins and rearing histories were used: a wheat-reared population (collected 2019 in Nanjing; >20 generations) and a rice-reared population (collected 2021 in Hangzhou; >10 generations). They were reciprocally transferred onto rice and wheat, establishing four host switching treatments (RR, RW, WR, WW). Second-instar larvae were exposed to LC(25) indoxacarb, after which we recorded larval and pupal development, fecundity and egg hatchability, and quantified six P450 genes (CmedCYP49A1, CmedCYP6AE30, CmedCYP12A2, CmedCYP301A1, CmedCYP4V2, and CmedCYP4AE76) and four JH-related genes (CmedAT, CmedJHAMT, CmedJHE, and CmedJHBP1). Host switching was associated with altered susceptibility, with RR more susceptible than RW and WW less susceptible than WR. Developmental effects were instar-specific. Pupal traits were affected by insecticide treatment and population origin, whereas reproductive traits were mainly affected by population origin. P450 and JH-related genes showed gene-specific expression patterns and were associated with some phenotypic changes. Gene expression correlated with phenotypes, suggesting potential trade-offs among detoxification, development, and reproduction. These laboratory findings indicate that host pre-adaptation and sublethal indoxacarb affect life-history traits in trait- and instar-specific ways. P450 and JH genes are potentially involved and merit functional validation, but field validation is needed before management application. FAU - Shen, Sunan AU - Shen S AD - Key Laboratory of Surveillance and Management of Invasive Alien Species, Guizhou Key Laboratory of Agricultural Biosecurity, Department of Biology and Engineering of Environment, Guiyang University, Guiyang 550005, China. AD - State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. FAU - Wen, Ronggen AU - Wen R AD - Key Laboratory of Surveillance and Management of Invasive Alien Species, Guizhou Key Laboratory of Agricultural Biosecurity, Department of Biology and Engineering of Environment, Guiyang University, Guiyang 550005, China. AD - State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. FAU - Yang, Yajun AU - Yang Y AUID- ORCID: 0000-0001-8524-5960 AD - State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. FAU - Xu, Hongxing AU - Xu H AD - State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. FAU - Chen, Yu AU - Chen Y AD - Shengzhou Agricultural Technology Extension Center, Shaoxing 312400, China. FAU - Li, Can AU - Li C AD - Key Laboratory of Surveillance and Management of Invasive Alien Species, Guizhou Key Laboratory of Agricultural Biosecurity, Department of Biology and Engineering of Environment, Guiyang University, Guiyang 550005, China. FAU - Lu, Yanhui AU - Lu Y AD - State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. FAU - Liu, Yan AU - Liu Y AD - Key Laboratory of Surveillance and Management of Invasive Alien Species, Guizhou Key Laboratory of Agricultural Biosecurity, Department of Biology and Engineering of Environment, Guiyang University, Guiyang 550005, China. FAU - Chen, Yuxuan AU - Chen Y AD - Key Laboratory of Surveillance and Management of Invasive Alien Species, Guizhou Key Laboratory of Agricultural Biosecurity, Department of Biology and Engineering of Environment, Guiyang University, Guiyang 550005, China. AD - State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. FAU - Lu, Zhongxian AU - Lu Z AD - State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. FAU - Wu, Yan AU - Wu Y AUID- ORCID: 0000-0002-1115-7332 AD - Key Laboratory of Surveillance and Management of Invasive Alien Species, Guizhou Key Laboratory of Agricultural Biosecurity, Department of Biology and Engineering of Environment, Guiyang University, Guiyang 550005, China. FAU - Guo, Jiawen AU - Guo J AUID- ORCID: 0000-0001-6743-1874 AD - State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. LA - eng GR - LMS26C140002/Zhejiang Provincial Natural Science Foundation/ GR - 2025SZRJJ2104/Natural Science Foundation of Hangzhou/ GR - CARS-01/China Agriculture Research System/ GR - KJLYRC-[2026]063/Guizhou Science and Technology Talent Program/ GR - GCC[2022]005/Guiyang Training Project for "Thousand Level" Talents in Guizhou Province/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705156 OTO - NOTNLM OT - Cnaphalocrocis medinalis OT - P450 OT - host switching OT - indoxacarb OT - juvenile hormone OT - sublethal effect COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:37 PHST- 2026/08/30 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - insects17101043 [pii] AID - insects-17-01043 [pii] AID - 10.3390/insects17101043 [doi] PST - epublish SO - Insects. 2026 Oct 10;17(10):1043. doi: 10.3390/insects17101043. PMID- 42940950 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 10 TI - Development of the First Cytochrome Oxidase I Barcode and Evidence for a Dominant Variant Associated with the Recent United States Invasion of the Pasture Mealybug Heliococcus summervillei (Hemiptera: Pseudococcidae) Complex. LID - 10.3390/insects17101040 [doi] LID - 1040 AB - The Heliococcus summervillei complex is an emerging invasive mealybug that causes severe dieback in grasses in pastures and turfgrass landscapes. It is widespread in Australia and has recently been detected across the Caribbean, Mexico, and the United States. Accurate mealybug identification is challenging because of cryptic and overlapping diagnostic characters and limited taxonomic expertise and literature, making molecular tools essential for regulatory diagnostics and management. We developed the first Cytochrome Oxidase I (COI) sequences for morphology-based Type A and Type B variants of the H. summervillei complex using the Jerry-Pat mid-COI fragment and used them to examine mitochondrial variation across available populations. COI sequences reveal approximately 10% mitochondrial split between the Type A and Type B variants. Phylogenetic, haplotype network, and genetic distance analyses show that all Type B populations share a single mitochondrial haplotype, consistent with a single dominant maternal lineage associated with a recent pasture mealybug invasion of the United States and Australia. Type A in Barbados contains two closely related haplotypes that may represent a historically stable mitochondrial variant corresponding to the holotype's morphological description of H. summervillei, based on morphology. Together, these results establish the first COI reference library for the H. summervillei complex, resolve mitochondrial lineage structure, and provide a practical tool for rapid identification and timely regulatory and pest management responses. FAU - Ahmed, Muhammad Z AU - Ahmed MZ AUID- ORCID: 0000-0003-2486-157X AD - Department of Plant and Environmental Sciences, Pee Dee Research and Education Center, Clemson University, Florence, SC 29506, USA. FAU - Tan, Peilin AU - Tan P AD - Department of Plant and Environmental Sciences, Pee Dee Research and Education Center, Clemson University, Florence, SC 29506, USA. FAU - Yadav, Nisha AU - Yadav N AUID- ORCID: 0009-0000-1597-536X AD - Department of Plant and Environmental Sciences, Pee Dee Research and Education Center, Clemson University, Florence, SC 29506, USA. FAU - Hauxwell, Caroline AU - Hauxwell C AUID- ORCID: 0000-0002-1681-9657 AD - School of Biology and Environmental Science, Queensland University of Technology, Brisbane, QLD 4000, Australia. FAU - Kerns, David L AU - Kerns DL AUID- ORCID: 0000-0002-2992-4851 AD - Department of Entomology, Texas A&M University, College Station, TX 77843, USA. FAU - Wilson, Blake AU - Wilson B AUID- ORCID: 0000-0001-9034-0612 AD - Department of Entomology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA. FAU - Quinn, Nicole AU - Quinn N AD - Indian River Research and Education Center, Department of Entomology and Nematology, Institute of Food and Agricultural Sciences, University of Florida, Fort Pierce, FL 34945, USA. FAU - Esquivel, Isaac L AU - Esquivel IL AD - North Florida Research and Education Center, Department of Entomology and Nematology, Institute of Food and Agricultural Sciences, University of Florida, Quincy, FL 32351, USA. FAU - Rustgi, Sachin AU - Rustgi S AUID- ORCID: 0000-0002-6881-9501 AD - Department of Plant and Environmental Sciences, Pee Dee Research and Education Center, Clemson University, Florence, SC 29506, USA. FAU - Hernandez-Europa, Ynna AU - Hernandez-Europa Y AUID- ORCID: 0009-0006-6752-0918 AD - School of Biology and Environmental Science, Queensland University of Technology, Brisbane, QLD 4000, Australia. FAU - Patrick, Darcy AU - Patrick D AD - School of Biology and Environmental Science, Queensland University of Technology, Brisbane, QLD 4000, Australia. LA - eng PT - Journal Article DEP - 20261010 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13704945 OTO - NOTNLM OT - biosecurity OT - genetics OT - invasive pest OT - pest management OT - regulatory diagnostics OT - turfgrass COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:37 PHST- 2026/08/22 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - insects17101040 [pii] AID - insects-17-01040 [pii] AID - 10.3390/insects17101040 [doi] PST - epublish SO - Insects. 2026 Oct 10;17(10):1040. doi: 10.3390/insects17101040. PMID- 42940948 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 9 TI - Economic Value of Apple Pollination in the European Union, 2000 to 2024. LID - 10.3390/insects17101038 [doi] LID - 1038 AB - Insect pollination of crops is an important ecosystem service. Estimating the economic value of this service is a significant challenge. Apple orchards were selected for this study because apple trees are the main crop in the European Union that requires insect pollination to produce a crop. The aim of the study was to estimate the economic value of pollination as an ecosystem service in European Union countries from 2000 to 2024. The calculations used the economic value of production dependence on insect pollination method. The study assumed a 90% impact of insect pollination on apple yields and the average annual apple prices obtained by producers in individual EU countries. Data from the FAOSTAT database were used. The results indicate that between 2000 and 2024, the economic value of apple orchard pollination averaged 7 billion USD annually, with a range of 5.5 to 9 billion USD. The average pollination value of 1 hectare of apple orchard in the European Union increased from almost 9000 USD in 2000 to almost 14,000 USD in 2024. Over the years and within individual countries, the value of pollination varied significantly (from 0.2 to 95.4 thousand USD per ha). The main factors influencing differences in pollination value across EU countries were apple yields and prices received by producers. FAU - Majewski, Janusz AU - Majewski J AUID- ORCID: 0000-0002-2221-462X AD - Department of International Economics and Agribusiness, Warsaw University of Life Sciences-SGGW, Nowoursynowska 166, 02-787 Warsaw, Poland. FAU - Sobolewska, Agnieszka AU - Sobolewska A AUID- ORCID: 0000-0003-4903-6701 AD - Department of International Economics and Agribusiness, Warsaw University of Life Sciences-SGGW, Nowoursynowska 166, 02-787 Warsaw, Poland. LA - eng PT - Journal Article DEP - 20261009 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705319 OTO - NOTNLM OT - European Union OT - apple orchards OT - economic value OT - economic value of insect pollination method OT - ecosystem service OT - insect pollination OT - pollination OT - pollination value COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:37 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - insects17101038 [pii] AID - insects-17-01038 [pii] AID - 10.3390/insects17101038 [doi] PST - epublish SO - Insects. 2026 Oct 9;17(10):1038. doi: 10.3390/insects17101038. PMID- 42940946 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 9 TI - Do Flower-Rich Windbreak Margins Enhance Predator Communities in Citrus Orchards? LID - 10.3390/insects17101036 [doi] LID - 1036 AB - Conservation biological control is central to sustainable pest management; however, its effectiveness depends on providing natural enemies with adequate floral resources and habitat structure. In Florida citrus, living windbreaks offer potential for improvement through floral resource provisioning. We evaluated whether flower-enriched windbreaks increase predator abundance and group richness in citrus orchards in north and central Florida over two years and assessed the role of wildflowers in supporting predator communities. In central Florida, treatment groves supported higher predator abundance and group richness across both sampling methods (vacuum and sticky trap) and both study years, particularly spiders, ants, minute pirate bugs, long-legged flies, and wasps. In north Florida, floral plantings did not significantly increase overall predator abundance or group richness, although some treatment effects emerged in the second year, suggesting delayed benefits related to plant establishment. Predator-plant interactions were region-specific: blanket flower attracted the highest proportion of predator visitors in both regions, accounting for 66% of visits by ants in central Florida and 62% of visits by syrphid flies in north Florida. Predator abundance was generally higher near windbreak edges in both regions. In central Florida, wasp abundance was significantly negatively associated with Asian citrus psyllid abundance, with each one-unit increase in wasp density associated with an approximately 2.7% decrease in expected psyllid abundance, whereas spider and ant associations were not statistically significant. Floral enhancements can strengthen predator communities, but outcomes vary across regions and years and depend on regional conditions, plant establishment, predator functional traits, and ecological interactions. Therefore, conservation biological control in citrus requires region-specific plant selection and consideration of ecological context beyond abundance alone. FAU - Exilien, Romain AU - Exilien R AUID- ORCID: 0009-0007-9932-9867 AD - Department of Entomology and Nematology, North Florida Research and Education Center, University of Florida, Quincy, FL 32351, USA. FAU - Chuang, Angela AU - Chuang A AD - Department of Environmental Sciences and Studies, Department of Biology, Washington College, Chestertown, MD 21620, USA. FAU - Blaauw, Brett AU - Blaauw B AUID- ORCID: 0000-0001-6165-2713 AD - Department of Entomology, University of Georgia, Athens, GA 30602, USA. FAU - Knox, Gary AU - Knox G AUID- ORCID: 0000-0002-6672-8593 AD - Department of Environmental Horticulture, North Florida Research and Education Center, University of Florida, Quincy, FL 32351, USA. FAU - Liesenfelt, Tracy AU - Liesenfelt T AUID- ORCID: 0009-0007-0511-8031 AD - Department of Entomology and Nematology, University of Florida, Gainesville, FL 32608, USA. FAU - Strzyzewski, Iris AU - Strzyzewski I AD - Department of Entomology and Nematology, North Florida Research and Education Center, University of Florida, Quincy, FL 32351, USA. FAU - Malfa, Kathi AU - Malfa K AD - Department of Entomology and Nematology, North Florida Research and Education Center, University of Florida, Quincy, FL 32351, USA. FAU - Diepenbrock, Lauren AU - Diepenbrock L AUID- ORCID: 0000-0003-2942-2066 AD - Department of Entomology and Nematology, Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, USA. FAU - Martini, Xavier AU - Martini X AUID- ORCID: 0000-0002-0556-3465 AD - Department of Entomology and Nematology, North Florida Research and Education Center, University of Florida, Quincy, FL 32351, USA. LA - eng GR - FP20-017/Southern SARE/ GR - FLA-CRC-006469/United States Department of Agriculture/ GR - FLA-NFC-006275/United States Department of Agriculture/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705309 OTO - NOTNLM OT - Asian citrus psyllid OT - Diaphorina citri OT - conservation biological control OT - floral resource OT - floral strips OT - habitat management OT - natural enemies OT - windbreaks COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:37 PHST- 2026/08/06 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - insects17101036 [pii] AID - insects-17-01036 [pii] AID - 10.3390/insects17101036 [doi] PST - epublish SO - Insects. 2026 Oct 9;17(10):1036. doi: 10.3390/insects17101036. PMID- 42940944 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 9 TI - Phytosanitary Monitoring of Major Lepidopteran Pest and Their Natural Enemies in Maize Agroecosystems of Southeastern Kazakhstan. LID - 10.3390/insects17101033 [doi] LID - 1033 AB - Information on the seasonal dynamics of major maize pests and naturally occurring parasitoids and predators in agroecosystems of southeastern Kazakhstan remains limited. Regional data on the seasonal occurrence of phytophagous insects and their natural enemies are needed to improve phytosanitary monitoring and support the development of environmentally oriented approaches to maize protection. The study was conducted from 2022 to 2025 in commercial maize fields in the Almaty and Zhetysu regions of southeastern Kazakhstan. Phytosanitary surveys included visual monitoring of pests and their developmental stages on selected plants, assessment of overwintering Ostrinia spp. populations in post-harvest maize residues, pheromone monitoring of the seasonal activity of Ostrinia spp. and Helicoverpa armigera, and recording of naturally occurring parasitoids and predators. The major lepidopteran pests recorded were Ostrinia spp. and H. armigera. Pheromone monitoring revealed year-to-year differences in the timing and magnitude of seasonal trap catches of both taxa. For Ostrinia spp., several periods of increased trap catches were recorded during the growing season, primarily from June to August, with the highest mean catch of 23 males per trap recorded in the third ten-day period of June 2024. For H. armigera, the timing of maximum trap catches varied among years, with the highest mean catch of 23 males per trap recorded in the first ten-day period of August 2025. Viable overwintering larvae of Ostrinia spp. were found in all 11 surveyed farms. Maize residues also contained larvae parasitized by Braconidae, tachinid pupae, and individuals showing signs of bacterial infection. During the growing season, representatives of Braconidae, Trichogrammatidae, Chrysopidae, Coccinellidae, and Tachinidae were recorded in maize agroecosystems. The findings provide a regional baseline for improving phytosanitary monitoring of maize in southeastern Kazakhstan. The observed year-to-year differences in seasonal trap catches highlight the importance of accounting for variation among individual growing seasons, while the naturally occurring parasitoids and predators recorded in this study warrant further investigation to assess their potential role in environmentally oriented maize pest management programs. FAU - Adilkhankyzy, Ainura AU - Adilkhankyzy A AD - Department of Horticulture, Plant Protection and Quarantine, Faculty of Agrobiology, Kazakh National Agrarian Research University, Almaty 050010, Kazakhstan. FAU - Alpysbayeva, Karlygash A AU - Alpysbayeva KA AUID- ORCID: 0000-0002-8962-384X AD - Department of Biological Plant Protection, Kazakh Research Institute of Plant Protection and Quarantine Named After Zh. Zhiyembayev, Almaty 050070, Kazakhstan. AD - Department of Technique and Technology of Food Production, International Engineering and Technological University, Almaty 050060, Kazakhstan. FAU - Tleubergenov, Khamitzhan M AU - Tleubergenov KM AUID- ORCID: 0000-0001-5786-2275 AD - Department of Biological Plant Protection, Kazakh Research Institute of Plant Protection and Quarantine Named After Zh. Zhiyembayev, Almaty 050070, Kazakhstan. FAU - Nurmanov, Bauyrzhan B AU - Nurmanov BB AD - Department of Biological Plant Protection, Kazakh Research Institute of Plant Protection and Quarantine Named After Zh. Zhiyembayev, Almaty 050070, Kazakhstan. FAU - Naimanova, Balzhan Zh AU - Naimanova BZ AD - Department of Biological Plant Protection, Kazakh Research Institute of Plant Protection and Quarantine Named After Zh. Zhiyembayev, Almaty 050070, Kazakhstan. FAU - Shissenbayeva, Nurgul Z AU - Shissenbayeva NZ AD - Department of Biological Plant Protection, Kazakh Research Institute of Plant Protection and Quarantine Named After Zh. Zhiyembayev, Almaty 050070, Kazakhstan. FAU - Agabek, Aidana B AU - Agabek AB AD - Department of Biological Plant Protection, Kazakh Research Institute of Plant Protection and Quarantine Named After Zh. Zhiyembayev, Almaty 050070, Kazakhstan. FAU - Tashigul, Nurzhan AU - Tashigul N AUID- ORCID: 0009-0002-3109-3708 AD - Department of Biological Plant Protection, Kazakh Research Institute of Plant Protection and Quarantine Named After Zh. Zhiyembayev, Almaty 050070, Kazakhstan. FAU - Seitzhan, Assel M AU - Seitzhan AM AD - Department of Biological Plant Protection, Kazakh Research Institute of Plant Protection and Quarantine Named After Zh. Zhiyembayev, Almaty 050070, Kazakhstan. FAU - Duissembekov, Bakhytzhan A AU - Duissembekov BA AD - Department of Biological Plant Protection, Kazakh Research Institute of Plant Protection and Quarantine Named After Zh. Zhiyembayev, Almaty 050070, Kazakhstan. FAU - Kaldybekkyzy, Gulzhan AU - Kaldybekkyzy G AD - Department of Biological Plant Protection, Kazakh Research Institute of Plant Protection and Quarantine Named After Zh. Zhiyembayev, Almaty 050070, Kazakhstan. LA - eng GR - AP27511331/Ministry of Science and Higher Education of the Republic of Kazakhstan/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705255 OTO - NOTNLM OT - Helicoverpa armigera OT - Kazakhstan OT - Ostrinia spp. OT - Zea mays OT - biological control OT - natural enemies OT - parasitoids OT - pheromone monitoring OT - predators OT - seasonal dynamics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:37 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - insects17101033 [pii] AID - insects-17-01033 [pii] AID - 10.3390/insects17101033 [doi] PST - epublish SO - Insects. 2026 Oct 9;17(10):1033. doi: 10.3390/insects17101033. PMID- 42940943 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 9 TI - Egg-Stage Daily Temperature Amplitude Constrains Adult Fitness and Population Growth in Tuta absoluta (Meyrick). LID - 10.3390/insects17101034 [doi] LID - 1034 AB - Daily temperature variation associated with climate change can strongly affect the survival, development, and population growth of insect species. However, whether the amplitude of daily temperature fluctuation experienced during the early development of insects leads to carry-over effects across the later life stages remains insufficiently understood. Using the invasive pest Tuta absoluta, we exposed the eggs to different thermal regimes under two mean-temperature backgrounds (22 and 25 degrees C) and measured the immediate egg-stage responses and the subsequent carry-over effects. The immediate responses depended on mean temperature, consistent with the nonlinear shape of thermal performance curves. At 22 degrees C, the wide amplitude accelerated egg development and increased variation in developmental duration without significantly affecting egg survival. At 25 degrees C, the moderate amplitude accelerated development, whereas the wide amplitude partially reversed this acceleration and produced a steeper age-dependent increase in mortality. Temperature conditions experienced during the egg stage also generated pronounced carry-over effects: wide amplitudes reduced larval survival, shortened female adult longevity, and decreased fecundity, whereas male longevity remained comparatively stable-a pattern consistent with sex-specific sensitivity of adult fitness to early thermal history. The wide amplitude during the egg stage also reduced the intrinsic rate of increase and net reproductive rate. Thus, the thermal regime during the egg stage can suppress population growth through cross-stage effects and sex-dependent fitness responses. Models based only on mean temperature may overestimate population growth potential and mischaracterize phenology. Incorporating egg-stage thermal history and daily temperature fluctuation into phenological and demographic models could improve pest forecasting and integrated pest management decisions under climate change. FAU - An, Xiao-Ning AU - An XN AD - Shanxi Agricultural University, Taiyuan 030031, China. FAU - Li, Yang AU - Li Y AD - Shanxi Agricultural University, Taiyuan 030031, China. FAU - Ding, Yue AU - Ding Y AD - Shanxi Agricultural University, Taiyuan 030031, China. FAU - Liu, Yun-Wei AU - Liu YW AD - Shanxi Agricultural University, Taiyuan 030031, China. FAU - Jia, Mei-Qi AU - Jia MQ AD - Shanxi Agricultural University, Taiyuan 030031, China. FAU - Li, Da-Qi AU - Li DQ AD - Shanxi Agricultural University, Taiyuan 030031, China. FAU - Liu, Wan-Xue AU - Liu WX AD - Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. FAU - Guo, Jian-Yang AU - Guo JY AUID- ORCID: 0009-0003-7759-0811 AD - Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. FAU - Xing, Kun AU - Xing K AD - Shanxi Agricultural University, Taiyuan 030031, China. LA - eng GR - 202402140601012/Key Research and Development Program Projects of Shanxi Province/ GR - 2025YFC2609100/National Key Research and Development Program/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705332 OTO - NOTNLM OT - Tuta absoluta OT - carry-over effects OT - daily temperature fluctuation OT - population growth OT - thermal performance curve COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:37 PHST- 2026/09/19 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - insects17101034 [pii] AID - insects-17-01034 [pii] AID - 10.3390/insects17101034 [doi] PST - epublish SO - Insects. 2026 Oct 9;17(10):1034. doi: 10.3390/insects17101034. PMID- 42940938 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 7 TI - Characterization of a Stably Maintained, Highly Beta-Cypermethrin-Resistant Strain of the Bird Cherry-Oat Aphid Rhopalosiphum padi (Hemiptera: Aphididae). LID - 10.3390/insects17101029 [doi] LID - 1029 AB - Insecticide resistance threatens effective pest management. In this study on Rhopalosiphum padi, a predominant wheat pest, a field-derived beta-cypermethrin-resistant strain (Beta-R strain) showed a 4623.50-fold resistance increase after 22 generations of beta-cypermethrin exposure and remained highly resistant (>4000-fold) after 40 generations without exposure. Beta-R strain exhibited a relative fitness of 0.92, high cross-resistance to lambda-cyhalothrin and bifenthrin, low-to-moderate resistance to sulfoxaflor, thiamethoxam, and imidacloprid, and negative or no resistance to omethoate and chlorpyrifos. The cytochrome P450 monooxygenases (P450s) and glutathione S-transferases (GSTs) contribute to this resistance. A homozygous spuer-kdr M918L mutation was identified in the IIS4-S6 region of the voltage-gated sodium channel gene in the Beta-R strain; however, transgenic Drosophila melanogaster assays showed that this mutation alone conferred only a 2.96-fold increase in beta-cypermethrin resistance. These findings show that resistant of R. padi to beta-cypermethrin can be retain high-level resistance without insecticide exposure, while also conferring high-level cross-resistance to other pyrethroids and low or negative resistance to other insecticides with different modes of action, with the resistance phenotype linked to upregulated P450/GST activities and an M918L mutation that makes only a minor contribution. These findings have significant implications for the management of resistance to pyrethroid insecticides in R. padi. FAU - Li, Xinan AU - Li X AUID- ORCID: 0000-0002-9080-9350 AD - Henan Engineering Technology Research Center for Major Invasive Alien Species Prevention and Control, School of Plant Protection and Environment, Henan Institute of Science and Technology, Xinxiang 453003, China. AD - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. FAU - Gong, Peipan AU - Gong P AD - The Ministry of Agriculture and Rural Affairs Key Laboratory of Integrated Pest Management of Tropical Crops, Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China. FAU - Zhao, Zilan AU - Zhao Z AD - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. FAU - Wang, Fengfan AU - Wang F AD - Henan Engineering Technology Research Center for Major Invasive Alien Species Prevention and Control, School of Plant Protection and Environment, Henan Institute of Science and Technology, Xinxiang 453003, China. AD - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. FAU - Li, Qiuchi AU - Li Q AD - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. FAU - Li, Xiangrui AU - Li X AD - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. FAU - Zhang, Yunhui AU - Zhang Y AD - State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. FAU - Zhu, Xun AU - Zhu X AUID- ORCID: 0000-0002-7538-1528 AD - State Key Laboratory for Quality and Safety of Agro-Products, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China. LA - eng GR - 32272604/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261007 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705059 OTO - NOTNLM OT - detoxifying enzymes OT - fitness cost OT - insecticide resistance OT - pyrethroids OT - voltage-gated sodium channel COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/07 CRDT- 2026/10/27 10:37 PHST- 2026/08/30 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - insects17101029 [pii] AID - insects-17-01029 [pii] AID - 10.3390/insects17101029 [doi] PST - epublish SO - Insects. 2026 Oct 7;17(10):1029. doi: 10.3390/insects17101029. PMID- 42940934 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 4 TI - CRISPR/Cas9-Mediated Knockout of the Chorion Gene s36 Impairs Ovarian Development and Abolishes Egg Hatching in the Melon Fly Zeugodacus cucurbitae. LID - 10.3390/insects17101024 [doi] LID - 1024 AB - The melon fly, Zeugodacus cucurbitae (Coquillett), is a globally invasive pest of cucurbit crops whose concealed larval feeding limits chemical control and has driven interest in reproduction-based management. The chorion gene s36 encodes an eggshell protein conserved among dipterans. However, whether s36 is required for female reproduction in Z. cucurbitae remains unknown. We generated a homozygous s36 mutant line by CRISPR/Cas9 and characterized its reproductive phenotype. Disruption of s36 strongly reduced transcript abundance and truncated the predicted protein. Mutant females showed elevated mortality after sexual maturation, fecundity was approximately halved, and none of the eggs laid hatched. Ovaries were markedly reduced in size and were scored as abnormal in every mutant female examined, and eggs were smaller, dull and shriveled rapidly in air, indicating compromised chorion integrity. Both vitellogenin genes were down-regulated in the ovary. Whole-body transcriptomics identified 179 differentially expressed genes enriched in lipid and hormone-related metabolism. Tissue-resolved RT-qPCR showed vitellogenin and yellow-g2 transcripts elevated in the fat body yet depleted in the ovary; whether this pattern reflects impaired delivery of yolk precursors to the oocyte or the secondary loss of the follicular tissue in which these genes are normally expressed remains to be determined. These findings identify s36 as required for normal ovarian maturation and egg viability in Z. cucurbitae, and as a candidate target for genetic control of this pest. FAU - Sun, Lina AU - Sun L AD - Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China. AD - School of Life Sciences, Hangzhou Normal University, Hangzhou 311121, China. FAU - Fan, Xinlin AU - Fan X AD - Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China. FAU - Chen, Ting AU - Chen T AD - Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China. FAU - Shi, Qingxing AU - Shi Q AD - Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China. FAU - Qi, Guojun AU - Qi G AUID- ORCID: 0000-0003-4269-2888 AD - Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China. FAU - Tang, Bin AU - Tang B AD - School of Life Sciences, Hangzhou Normal University, Hangzhou 311121, China. FAU - Chen, Jie AU - Chen J AUID- ORCID: 0000-0002-7029-6674 AD - Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China. LA - eng GR - NYQS202626/Special Funding for the Construction of the High-Level Academy of Agricultural Sciences/ GR - KLGPFS-2026-PT-01/Open Project of Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs/ PT - Journal Article DEP - 20261004 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13704921 OTO - NOTNLM OT - Zeugodacus cucurbitae OT - eggshell permeability OT - female sterility OT - genetic pest control OT - genome editing OT - oogenesis OT - vitellogenin COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/04 CRDT- 2026/10/27 10:37 PHST- 2026/09/08 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/04 00:00 [pmc-release] AID - insects17101024 [pii] AID - insects-17-01024 [pii] AID - 10.3390/insects17101024 [doi] PST - epublish SO - Insects. 2026 Oct 4;17(10):1024. doi: 10.3390/insects17101024. PMID- 42940933 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 3 TI - Identification of Genes Encoding Seminal Fluid Proteins in Coccinella septempunctata and Functional Analysis of the Gene Encoding Trypsin. LID - 10.3390/insects17101023 [doi] LID - 1023 AB - In this study, transcriptomic and proteomic analyses were used to identify genes encoding seminal fluid proteins in Coccinella septempunctata L. A total of 65 SFPs domains containing secretory signal peptides were identified, including trypsin, Kazal_2, His_Phos_2, Peptidase_M14, lipase, Kunitz_BPTI, Ribonuc_2-5A, and Gly-co_transf_90; an additional 22 signal peptide-containing domains were identified that lacked annotations in the Pfam database. Transcript levels of the 65 SFPs accounted for 87.58% of the total expression levels of 211 specifically expressed proteins in the male reproductive tract (MRT). RNA interference (RNAi) was used to analyze the function of the SFP trypsin. RNAi-mediated knockdown of the seminal fluid trypsin gene in male C. septempunctata, followed by mating with females, significantly reduced female fecundity and egg hatching rates. This study enriches our knowledge of insect SFPs and provides a theoretical basis for the artificial rearing of C. septempunctata. FAU - Cheng, Ying AU - Cheng Y AUID- ORCID: 0000-0003-1121-2722 AD - Institute of Plant Protection, Guizhou Provincial Academy of Agricultural Sciences, Guiyang 550006, China. AD - Guizhou Branch of State Key Laboratory for Biology of Plant Diseases and Insect Pests, Guiyang 550006, China. AD - Guizhou Provincial Laboratory of Green Technology and Application Engineering of Plant Protection, Guiyang 550006, China. FAU - Zhou, Yuhang AU - Zhou Y AD - Institute of Plant Protection, Guizhou Provincial Academy of Agricultural Sciences, Guiyang 550006, China. AD - Guizhou Branch of State Key Laboratory for Biology of Plant Diseases and Insect Pests, Guiyang 550006, China. AD - Guizhou Provincial Laboratory of Green Technology and Application Engineering of Plant Protection, Guiyang 550006, China. FAU - Dai, Changgeng AU - Dai C AD - Institute of Plant Protection, Guizhou Provincial Academy of Agricultural Sciences, Guiyang 550006, China. AD - Guizhou Branch of State Key Laboratory for Biology of Plant Diseases and Insect Pests, Guiyang 550006, China. AD - Guizhou Provincial Laboratory of Green Technology and Application Engineering of Plant Protection, Guiyang 550006, China. FAU - Ran, Haiyan AU - Ran H AUID- ORCID: 0009-0005-9445-8104 AD - Institute of Plant Protection, Guizhou Provincial Academy of Agricultural Sciences, Guiyang 550006, China. AD - Guizhou Branch of State Key Laboratory for Biology of Plant Diseases and Insect Pests, Guiyang 550006, China. AD - Guizhou Provincial Laboratory of Green Technology and Application Engineering of Plant Protection, Guiyang 550006, China. FAU - Li, Cao AU - Li C AD - Guizhou Provincial Pollution-Free Engineering Center of Plant Protection, Guiyang 550006, China. LA - eng GR - 32460711/National Natural Science Foundation of China/ GR - QianKeHe ZSYS [2025] 024/Guizhou Key Laboratory of Agricultural Biosecurity/ GR - QianKeHeFuQi [2023]011/Innovative Capabilities Buildup of Green Prevention and Control for Invasive Species in Agriculture/ PT - Journal Article DEP - 20261003 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705487 OTO - NOTNLM OT - ladybug OT - proteome OT - seminal fluid protein OT - transcriptome OT - trypsin COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/03 CRDT- 2026/10/27 10:37 PHST- 2026/08/11 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - insects17101023 [pii] AID - insects-17-01023 [pii] AID - 10.3390/insects17101023 [doi] PST - epublish SO - Insects. 2026 Oct 3;17(10):1023. doi: 10.3390/insects17101023. PMID- 42940932 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 3 TI - Population Genomic Insights into Genetic Differentiation and Environmental Adaptation of the Asian Honey Bee (Apis cerana) in Sichuan, China. LID - 10.3390/insects17101022 [doi] LID - 1022 AB - The Asian honey bee (Apis cerana) is a key native pollinator in Asia, contributing substantially to both natural and agricultural ecosystems. However, climate change and human activities increasingly threaten its pollination services and long-term survival. Understanding patterns of genetic differentiation and local adaptation is therefore essential for protecting native populations. Sichuan Province in China, with its complex mountains, deep valleys, and pronounced climatic heterogeneity, offers an excellent natural laboratory for studying these processes in A. cerana. We collected 139 worker bees from 22 locations across Sichuan and generated whole-genome resequencing data. By integrating these data with genomes from eight well-established subspecies, we show that bees from Xinlong, Jiuzhaigou, and Kangding form three genetically differentiated populations that are distinct from all known subspecies. These populations display moderate to high genetic differentiation and unique demographic histories relative to previously described subspecies. Selective-sweep analyses further identified 200-300 candidate genes under positive selection in each population. These genes are enriched in functions related to chemical sensing, flight musculature, cell-cycle regulation, osmotic balance, and stress response, consistent with adaptation to patchy nectar resources, a short spring reproductive window, and large diurnal temperature swings. Our findings uncover three previously unrecognized genetic resources of A. cerana and provide genomic insights into their local adaptation, offering a valuable foundation for conservation and breeding programs. FAU - Xu, Xiangcheng AU - Xu X AD - College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. FAU - Ma, Dingyuan AU - Ma D AD - College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. FAU - Liu, Bingrong AU - Liu B AD - College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. FAU - Yang, Mingxian AU - Yang M AD - College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. FAU - Lai, Kang AU - Lai K AD - Sichuan Provincial Animal Husbandry Master Station, Chengdu 610041, China. FAU - Yang, Jiandong AU - Yang J AD - College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China. LA - eng GR - 2021YFYZ0004/Science and Technology Department of Sichuan Province/ PT - Journal Article DEP - 20261003 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705124 OTO - NOTNLM OT - Apis cerana OT - environmental adaptation OT - genetic differentiation OT - population genomics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/03 CRDT- 2026/10/27 10:37 PHST- 2026/07/31 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - insects17101022 [pii] AID - insects-17-01022 [pii] AID - 10.3390/insects17101022 [doi] PST - epublish SO - Insects. 2026 Oct 3;17(10):1022. doi: 10.3390/insects17101022. PMID- 42940929 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 2 TI - Large Coccinellidae Collected in the Nebraska Panhandle Reveal High Diversity, Rare Species, and Undocumented Plant Associations. LID - 10.3390/insects17101018 [doi] LID - 1018 AB - Museum records and recent field surveys provide new insights into the diversity, collection methods, seasonal ecology, and plant associations of lady beetles (Coleoptera: Coccinellidae) in western Nebraska. More than 2500 specimens exist, with data on collection methods for 1882 specimens representing museum holdings and surveys conducted between 2012 and 2025. Most specimens were obtained by hand collection (1244; 66%), followed by sweep net sampling (292; 15%) and blacklight traps (261; 13.8%), whereas yellow pan traps produced 85 (4.5%) of all specimens. These proportions represent the composition of the collection and should not be interpreted as estimates of relative sampling efficiency because effort was not standardized among methods. More than half of all museum specimens of Adalia bipunctata, Harmonia axyridis, Hippodamia tredecimpunctata tibialis Timberlake, and Myzia interrupta (Casey) originated from a single continuously operated blacklight trap, while 26% of Coccinella transversoguttata were captured in yellow pan traps. Plant associations were documented for 1070 specimens and revealed recurring species-specific patterns in available records. Notably, 50% of Coleomegilla maculata lengi were collected on knotweeds (Polygonum spp.), 27% of Hippodamia parenthesis were associated with alfalfa, and two Scymnus species were collected from elm. Native sunflowers (Helianthus spp.) were particularly important, with over 75% of Coccinella monticola, C. novemnotata, and Hippodamia convergens collected apparently feeding on this host. Observations of lady beetles associated with sunflowers, in the absence of aphids or other apparent prey, suggest late-season phytophagy or exploitation of plant exudates rather than predation by adult beetles. Focused surveys with broadly similar search effort revealed striking interannual variation in detections, with several species frequently encountered in 2024 detected infrequently or not detected in 2025. Collectively, these records document the persistence of rare native coccinellids in western Nebraska, identify potentially important plant associations and detection methods, and provide baseline occurrence and natural-history information for future standardized monitoring. FAU - Brust, Mathew L AU - Brust ML AD - Department of Biology, Chadron State College, Chadron, NE 69337, USA. FAU - Hesler, Louis S AU - Hesler LS AUID- ORCID: 0000-0002-6469-2865 AD - Hesler Entomological Research & Consulting, Jackson, MS 39211, USA. FAU - Hoback, William Wyatt AU - Hoback WW AUID- ORCID: 0000-0002-8927-9102 AD - Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK 74078, USA. LA - eng PT - Journal Article DEP - 20261002 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705464 OTO - NOTNLM OT - biological control OT - declining OT - invasive OT - ladybug OT - occurrence records OT - polyphagy OT - predator OT - sampling methods COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/02 CRDT- 2026/10/27 10:37 PHST- 2026/09/14 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/02 00:00 [pmc-release] AID - insects17101018 [pii] AID - insects-17-01018 [pii] AID - 10.3390/insects17101018 [doi] PST - epublish SO - Insects. 2026 Oct 2;17(10):1018. doi: 10.3390/insects17101018. PMID- 42940928 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 2 TI - First Detection and Delimiting Survey of the Emerald Ash Borer (Agrilus planipennis Fairmaire, 1888 (Coleoptera: Buprestidae)) in Slovakia. LID - 10.3390/insects17101019 [doi] LID - 1019 AB - The emerald ash borer, Agrilus planipennis Fairmaire, 1888 (Coleoptera: Buprestidae), is an invasive pest in Europe and North America, responsible for extensive mortality of ash (Fraxinus spp.). This study reports its first confirmed occurrence in Slovakia and summarises the subsequent delimiting survey. On 25 June 2026, specimens morphologically resembling A. planipennis were collected from a monitoring trap in a park in Streda nad Bodrogom, south-eastern Slovakia. Eighteen adults were identified as A. planipennis by the national quarantine diagnostics laboratory, with confirmation by the European Union Reference Laboratory in France. Initial field inspections revealed characteristic galleries and D-shaped exit holes on ash trees at the detection site, in nearby forests, and along roads within approximately 7 km. By 31 July 2026, 2250 survey points had been recorded, of which 149 showed evidence of A. planipennis, including adults, larvae, galleries, or exit holes. Old galleries together with living larvae indicate that the pest had probably been established in Slovakia for several years before detection in 2026. Symptom assessment was complicated by widespread ash dieback caused by Hymenoscyphus fraxineus (T. Kowalski) Baral, Queloz & Hosoya, 2014. FAU - Gubka, Andrej AU - Gubka A AUID- ORCID: 0000-0003-0408-6043 AD - National Forest Centre, T. G. Masaryka 2175/22, 960 01 Zvolen, Slovakia. FAU - Dubec, Marcel AU - Dubec M AD - National Forest Centre, T. G. Masaryka 2175/22, 960 01 Zvolen, Slovakia. FAU - Nikolov, Christo AU - Nikolov C AD - National Forest Centre, T. G. Masaryka 2175/22, 960 01 Zvolen, Slovakia. FAU - Darnadyova, Kristina AU - Darnadyova K AD - Laboratory of Quarantine Diagnostics, Central Control and Testing Institute in Agriculture, Letna 3, 041 39 Kosice, Slovakia. FAU - Kurhajcova, Ivana AU - Kurhajcova I AD - Central Control and Testing Institute in Agriculture, Department of Plant Protection, Matuskova 21, 833 16 Bratislava, Slovakia. FAU - Lucaj, Maros AU - Lucaj M AD - Central Control and Testing Institute in Agriculture, Department of Plant Protection, Matuskova 21, 833 16 Bratislava, Slovakia. FAU - Galko, Juraj AU - Galko J AD - National Forest Centre, T. G. Masaryka 2175/22, 960 01 Zvolen, Slovakia. FAU - Kunca, Andrej AU - Kunca A AD - National Forest Centre, T. G. Masaryka 2175/22, 960 01 Zvolen, Slovakia. FAU - Lalik, Michal AU - Lalik M AUID- ORCID: 0000-0003-1794-5222 AD - National Forest Centre, T. G. Masaryka 2175/22, 960 01 Zvolen, Slovakia. FAU - Vakula, Jozef AU - Vakula J AUID- ORCID: 0000-0001-8662-1817 AD - National Forest Centre, T. G. Masaryka 2175/22, 960 01 Zvolen, Slovakia. FAU - Rell, Slavomir AU - Rell S AD - National Forest Centre, T. G. Masaryka 2175/22, 960 01 Zvolen, Slovakia. FAU - Longauerova, Valeria AU - Longauerova V AD - National Forest Centre, T. G. Masaryka 2175/22, 960 01 Zvolen, Slovakia. FAU - Leontovyc, Roman AU - Leontovyc R AD - National Forest Centre, T. G. Masaryka 2175/22, 960 01 Zvolen, Slovakia. FAU - Zubrik, Milan AU - Zubrik M AD - National Forest Centre, T. G. Masaryka 2175/22, 960 01 Zvolen, Slovakia. LA - eng GR - 101059552/European Commission/ GR - 313011S735/European Commission/ GR - APVV-23-0156/Slovak Research and Development Agency/ GR - APVV-24-0425/Slovak Research and Development Agency/ GR - APVV-25-0060/Slovak Research and Development Agency/ GR - APVV-25-0731/Slovak Research and Development Agency/ GR - APVV-25-0088/Slovak Research and Development Agency/ GR - 08V0301/Ministry of Agriculture and Rural Development of the Slovak Republic/ PT - Journal Article DEP - 20261002 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705454 OTO - NOTNLM OT - Agrilus planipennis OT - Fraxinus OT - Slovakia OT - delimiting survey OT - emerald ash borer OT - first record OT - invasive alien species OT - quarantine pest COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/02 CRDT- 2026/10/27 10:37 PHST- 2026/09/11 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/02 00:00 [pmc-release] AID - insects17101019 [pii] AID - insects-17-01019 [pii] AID - 10.3390/insects17101019 [doi] PST - epublish SO - Insects. 2026 Oct 2;17(10):1019. doi: 10.3390/insects17101019. PMID- 42940925 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 1 TI - Tephritid Fly Abundance and Inflorescence Herbivory on a High Alpine Thistle Vary with Environment. LID - 10.3390/insects17101015 [doi] LID - 1015 AB - Little is known about insect-plant interactions in high alpine environments. We studied the insect herbivore feeding guild in inflorescences of Cirsium funkiae (Asteraceae), an alpine thistle endemic to the southern Rocky Mountains, USA. We asked whether site (open talus above treeline, mid-elevation wet meadow, or lower-elevation forest edge just below treeline) and plant characteristics affect insect abundance, guild composition, and intensity of herbivory. We dissected flower heads collected at season's end to quantify fly occurrence and measure herbivory damage. Three tephritid flies-Chaetostomella undosa, Paracantha gentilis, and Terellia occidentalis-strongly dominated the herbivore guild. All three species differed in abundance and composition across habitat sites. Chaetostomella dominated in the high, open talus habitat; Terellia peaked in the mid-elevation meadow site; and Paracantha dominated in the lower forest edge site. While Terellia abundance was strongly associated with flower head diameter, abundances of Paracantha and Chaetostomella were only weakly associated with flower head size, suggesting species-specific flower-head selection. Paracantha and Terellia showed elevated co-occurrence at the mid-elevation alpine meadow site, indicating shared distributional preferences likely related to their occurrence on thistles at lower elevations in this region. Unexpectedly, seed damage was extremely high (91.5-98.5%) across all three sites and highest in the characteristic habitat, open high talus slopes. Despite uniformly high seed damage, the low Forest Edge plants had significantly more viable seeds than did the High site plants (8.2% vs. 1.6%), and the difference was associated with differences in guild composition. These findings demonstrate that tephritid flies dominate floral herbivory on the high alpine Cirsium funkiae, that site differences can shape fly feeding guild structure, and that they have a measurable negative impact on plant seed reproductive success in high alpine environments. FAU - Reville, Raven A AU - Reville RA AUID- ORCID: 0009-0007-9679-4448 AD - Department of Biological Sciences, Ecosystem Science and Stewardship Program, Colorado Mountain College, Breckenridge, CO 80424, USA. FAU - Schultz, Emily L AU - Schultz EL AUID- ORCID: 0000-0002-0426-2513 AD - Department of Biological Sciences, Ecosystem Science and Stewardship Program, Colorado Mountain College, Breckenridge, CO 80424, USA. FAU - Louda, Svata M AU - Louda SM AD - School of Biological Sciences, University of Nebraska, Lincoln, NE 68588, USA. LA - eng PT - Journal Article DEP - 20261001 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705526 OTO - NOTNLM OT - Cirsium OT - alpine herbivory OT - floral herbivory OT - guild structure OT - habitat partitioning OT - pre-dispersal seed predation OT - seed reproductive success OT - tephritid flies COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:37 PHST- 2026/08/28 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - insects17101015 [pii] AID - insects-17-01015 [pii] AID - 10.3390/insects17101015 [doi] PST - epublish SO - Insects. 2026 Oct 1;17(10):1015. doi: 10.3390/insects17101015. PMID- 42940923 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Oct 1 TI - Seasonal Dynamics, Botanical Origin, and Yield of Apis mellifera Propolis in the Mexican Altiplano: Influence of Precipitation, Phenology, and Harvesting Methods. LID - 10.3390/insects17101013 [doi] LID - 1013 AB - Propolis yield and composition fluctuate with environmental factors and hive management. In a two-year longitudinal study (2022-2024), we evaluated the seasonal dynamics of propolis production across two apiaries in the Mexican Altiplano during the dry and rainy seasons. We conducted monthly harvesting using three collector types: black polyurethane collectors (G1), beige polyurethane collectors (G2), and flexible polyethylene mosquito mesh (G3), distributed among 24 standardized Apis mellifera colonies (n = 8 per treatment; 576 repeated monthly harvests). Palynological analysis identified 39 species belonging to 38 genera and 23 families, with a clear predominance of herbaceous and ruderal forms (Brassica rapa as the main pollen type). The overall mean yield was 9.02 +/- 0.58 g/colony/month. Linear mixed-effects models (LMMs) revealed significant temporal variation by month (F(11),(231) = 28.45, p < 0.001) and season (F(1),(21) = 14.12, p = 0.001). Propolis biomass showed a clear bimodal pattern, peaking in April (20.25 g/colony) during vegetative bud burst, dropping in July (6.25 g/colony), and rebounding in September-October (9.77-9.92 g/colony), coinciding with leaf senescence and pre-winter nest sealing. Monthly production showed a moderate negative linear correlation with cumulative precipitation (r = -0.422, p = 0.040, R(2) = 0.178). Conversely, collector design did not result in statistically significant differences in harvested biomass (G1: 6.88 g, G2: 8.98 g, G3: 11.21 g; F(2),(21) = 2.14, p = 0.142). These findings demonstrate that propolis collection in the Mexican Altiplano is primarily synchronized with regional plant phenology and constrained by precipitation regimes rather than collector design. Consequently, the practical selection of harvesting devices should be guided by operational handling efficiency, economic cost, and raw product cleanliness, rather than expectations of higher quantitative yield. FAU - Alcivar-Saldana, Jose Juan AU - Alcivar-Saldana JJ AUID- ORCID: 0000-0003-2803-7705 AD - Departamento de Ciencias Pecuarias, Facultad de Estudios Superiores Cuautitlan, Universidad Nacional Autonoma de Mexico (UNAM), Carretera Cuautitlan-Teoloyucan km. 2.5, Colonia San Sebastian Xhala, Cuautitlan Izcalli C.P. 54714, Estado de Mexico, Mexico. FAU - Rodriguez-Monroy, Marco Aurelio AU - Rodriguez-Monroy MA AUID- ORCID: 0000-0002-6780-0710 AD - Laboratorio de Investigacion Biomedica en Productos Naturales, Carrera de Medicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de Mexico (UNAM), Avenida de los Barrios Numero 1, Colonia Los Reyes Iztacala, Tlalnepantla de Baz C.P. 54090, Estado de Mexico, Mexico. FAU - Corona-Gomez, Lysett AU - Corona-Gomez L AD - Departamento de Ciencias Biologicas, Facultad de Estudios Superiores Cuautitlan, Universidad Nacional Autonoma de Mexico (UNAM), Carretera Cuautitlan-Teoloyucan km. 2.5, Colonia San Sebastian Xhala, Cuautitlan Izcalli C.P. 54714, Estado de Mexico, Mexico. FAU - Diaz-Sanchez, Victor Manuel AU - Diaz-Sanchez VM AUID- ORCID: 0000-0002-9295-8819 AD - Departamento de Ciencias Pecuarias, Facultad de Estudios Superiores Cuautitlan, Universidad Nacional Autonoma de Mexico (UNAM), Carretera Cuautitlan-Teoloyucan km. 2.5, Colonia San Sebastian Xhala, Cuautitlan Izcalli C.P. 54714, Estado de Mexico, Mexico. FAU - Gonzalez-Toimil, Manuel Andres AU - Gonzalez-Toimil MA AD - Centro de Ensenanza Agropecuaria, FES Cuautitlan, Carretera Cuautitlan-Teoloyucan km. 2.5, Colonia San Sebastian Xhala, Cuautitlan Izcalli C.P. 54714, Estado de Mexico, Mexico. FAU - Garcia-Cedillo, Noe AU - Garcia-Cedillo N AD - Subdireccion de Impulso a la Produccion Acuicola, Avicola y Apicola, Secretaria del Campo (SECAMPO), Conjunto SEDAGRO Rancho San Lorenzo S/N, Manzana 001, San Lorenzo, Coacalco C.P. 52140, Estado de Mexico, Mexico. FAU - Canales-Martinez, Maria Margarita AU - Canales-Martinez MM AUID- ORCID: 0000-0002-4292-0476 AD - Laboratorio de Farmacognosia, Unidad de Biotecnologia y Prototipos (UBIPRO), Facultad de Estudios Superiores Iztacala, Universidad Nacional Autonoma de Mexico (UNAM), Avenida de los Barrios Numero 1, Colonia Los Reyes Iztacala, Tlalnepantla de Baz C.P. 54090, Estado de Mexico, Mexico. LA - eng GR - UNAM PAPIIT IN208125/Universidad Nacional Autonoma de Mexico/ PT - Journal Article DEP - 20261001 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705010 OTO - NOTNLM OT - Apis mellifera OT - botanical origin OT - melissopalynology OT - plant phenology OT - propolis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:37 PHST- 2026/08/19 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - insects17101013 [pii] AID - insects-17-01013 [pii] AID - 10.3390/insects17101013 [doi] PST - epublish SO - Insects. 2026 Oct 1;17(10):1013. doi: 10.3390/insects17101013. PMID- 42940919 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Sep 30 TI - Beyond Algorithmic Accuracy: Field Readiness, Ecological Intelligence and Real-World Validation of Artificial Intelligence for Insect Pest Management. LID - 10.3390/insects17101009 [doi] LID - 1009 AB - Artificial intelligence (AI) is rapidly reshaping insect pest identification, monitoring, and decision support, yet field maturity is often judged from algorithmic accuracy on curated or internally split datasets. Such evidence is insufficient for integrated pest management (IPM), where decisions depend on pest abundance, crop phenology, natural enemies, weather, economic thresholds, treatment costs, and asymmetric error consequences. This critical review synthesizes the literature on computer vision, automated counting, smart traps, Internet of Things (IoT), edge AI, multimodal sensing, population forecasting, uncertainty-aware AI, decision support, and precision intervention. We examine the laboratory-to-field generalization gap, small-object and long-tailed recognition, ecological incompleteness of pest-only datasets, sensor and trap bias, domain shift, calibration, human oversight, and operational constraints. We propose layered evaluation metrics spanning technical, quantitative, ecological, generalization, operational, decision, and outcome performance, and introduce a six-level Pest-AI Readiness Framework (PARF), from laboratory recognition to closed-loop precision management. We argue that future pest-AI systems should integrate visual observations with weather, crop phenology, trap history, and natural-enemy information, quantify uncertainty, support expert escalation, and undergo prospective external validation to generate timely, selective, and ecologically sound IPM decisions. FAU - Akbal, Eda Budak AU - Akbal EB AD - Vocational School of Agriculture, Dicle University, Diyarbakir 21280, Turkiye. FAU - Bayhan, Erol AU - Bayhan E AUID- ORCID: 0000-0002-7579-586X AD - Department of Plant Protection, Faculty of Agriculture, Dicle University, Diyarbakir 21280, Turkiye. LA - eng GR - TARIM-MYO.26.002/Dicle University/ PT - Journal Article PT - Review DEP - 20260930 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13704983 OTO - NOTNLM OT - artificial intelligence OT - computer vision OT - decision support OT - insect pests OT - integrated pest management OT - smart traps COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:37 PHST- 2026/08/28 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - insects17101009 [pii] AID - insects-17-01009 [pii] AID - 10.3390/insects17101009 [doi] PST - epublish SO - Insects. 2026 Sep 30;17(10):1009. doi: 10.3390/insects17101009. PMID- 42940918 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Sep 30 TI - Global Future Modeling of the Monophagous Pest Pagiophloeus tsushimanus (Coleoptera: Curculionidae): An Integrated Approach Using Random Forest and the CLIMEX Model. LID - 10.3390/insects17101008 [doi] LID - 1008 AB - Pagiophloeus tsushimanus Morimoto, 1982, a camphor tree weevil, is an emerging invasive wood-boring pest that has resulted in a progressive decline of Cinnamomum camphora plantations in affected areas. Native to Tsushima Island, Japan, P. tsushimanus has also spread to eastern China. With global climate change, the geographic range, occurrence frequency, and severity of this pest are continuously increasing, particularly in affected areas of China. Therefore, to limit the continued spread of this pest, the Random Forest (RF) algorithm and the CLIMEX model were combined to map the potential global distribution of P. tsushimanus. The results indicated that the potential range of C. camphora was jointly regulated by moisture availability (BIO18) and human modification indicators (PD and gHM). In contrast, the geographic distribution limits of P. tsushimanus were primarily defined by cold stress thresholds at higher latitudes and by heat and dry stress limitations in its traditional subtropical core range. Under current climatic conditions, the host-constrained suitable habitat of P. tsushimanus covered 4238.03 x 10(4) km(2), mainly in East Asia, the southeastern United States, southeastern South America, and the Mediterranean coast of Europe. Under future climate change scenarios, P. tsushimanus and its host, C. camphora, exhibit a highly convergent poleward range expansion. New suitability hotspots for P. tsushimanus were projected to emerge in central and northern China, western and central Europe, and the northeastern United States. Owing to increased heat and drought stress in low-latitude subtropical core areas, the global suitable area under future climate change contracted by 17.99% overall, and highly suitable habitat decreased by 39.22%. The suitability maps of P. tsushimanus generated under host-constrained conditions in this study provide a potential early-warning layer for global forest health. We recommend that forestry departments in newly suitable temperate zones implement strict phytosanitary inspections of imported C. camphora nursery stock and that the timber and forest-product industries establish active monitoring programs to prevent the accidental introduction and establishment of this cryptic wood-boring pest. FAU - Wu, Qiang AU - Wu Q AD - Hubei Key Laboratory of Biological Resources Protection and Utilization, Hubei Minzu University, Enshi 445000, China. AD - College of Forestry and Horticulture, Hubei Minzu University, Enshi 445000, China. FAU - Xiao, Kaitong AU - Xiao K AD - Hubei Key Laboratory of Biological Resources Protection and Utilization, Hubei Minzu University, Enshi 445000, China. AD - College of Forestry and Horticulture, Hubei Minzu University, Enshi 445000, China. FAU - Deng, Xiaosu AU - Deng X AD - Hubei Key Laboratory of Biological Resources Protection and Utilization, Hubei Minzu University, Enshi 445000, China. AD - College of Forestry and Horticulture, Hubei Minzu University, Enshi 445000, China. FAU - Hu, Haijian AU - Hu H AD - Hubei Key Laboratory of Biological Resources Protection and Utilization, Hubei Minzu University, Enshi 445000, China. AD - College of Forestry and Horticulture, Hubei Minzu University, Enshi 445000, China. FAU - Li, Juan AU - Li J AD - Hubei Key Laboratory of Biological Resources Protection and Utilization, Hubei Minzu University, Enshi 445000, China. AD - College of Forestry and Horticulture, Hubei Minzu University, Enshi 445000, China. FAU - Lin, Zhenxi AU - Lin Z AD - Hubei Key Laboratory of Biological Resources Protection and Utilization, Hubei Minzu University, Enshi 445000, China. AD - College of Forestry and Horticulture, Hubei Minzu University, Enshi 445000, China. FAU - Ning, Hang AU - Ning H AD - Hubei Key Laboratory of Biological Resources Protection and Utilization, Hubei Minzu University, Enshi 445000, China. AD - College of Forestry and Horticulture, Hubei Minzu University, Enshi 445000, China. LA - eng GR - 2024AFB534/Science and Technology Department of Hubei Province/ PT - Journal Article DEP - 20260930 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705089 OTO - NOTNLM OT - Cinnamomum camphora OT - biosecurity OT - host plant constraint OT - invasive weevil OT - phytosanitary risk OT - shared socioeconomic pathways COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:37 PHST- 2026/09/10 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - insects17101008 [pii] AID - insects-17-01008 [pii] AID - 10.3390/insects17101008 [doi] PST - epublish SO - Insects. 2026 Sep 30;17(10):1008. doi: 10.3390/insects17101008. PMID- 42940913 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Sep 28 TI - Potential Geographical Distribution Prediction and Ecological Niche Change Analysis of Pissodes castaneus. LID - 10.3390/insects17101002 [doi] LID - 1002 AB - Pissodes castaneus is an internationally quarantined pest that damages various pine species. The global distribution patterns and invasion potential of P. castaneus have not been systematically assessed, thus leaving relevant prevention and control systems without a theoretical foundation. In this study, we used an optimized MaxEnt model to predict the global suitable areas of P. castaneus under current and future climate conditions. At the same time, it assesses the changes in the ecological niche of P. castaneus under climate change. The results indicate that the AUC values of the MaxEnt model exceeded 0.9 under both current and future climate scenarios, indicating high predictive accuracy. Under current climate conditions, the total global potential suitable area for P. castaneus is 1.08 x 10(7) km(2). The highly suitable areas are mainly distributed in Western Europe, northern Turkey, southeastern Australia, the west coast of North America, and the east coast of South America. Under the two future climate scenarios (SSP126 and SSP585), the total suitable area initially decreases and then increases, reaching its maximum during 2081-2100. Compared with current conditions, suitable areas shift northward in the Northern Hemisphere. Under both the SSP126 and SSP585 scenarios, although its niche remains highly conservative relative to current conditions, different emission scenarios may affect the niche dynamics of P. castaneus. This study reveals the spatiotemporal dynamics of suitable areas and niche characteristics of P. castaneus on a global scale, providing a basis for worldwide quarantine and inspection efforts and offering scientific data support for future prevention and control of its spread. FAU - Li, Yuxuan AU - Li Y AD - Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China. AD - Sino-France Joint Laboratory for Invasive Forest Pests in Eurasia, Beijing Forestry University, Beijing 100083, China. FAU - Sun, Dongrui AU - Sun D AD - Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China. AD - Sino-France Joint Laboratory for Invasive Forest Pests in Eurasia, Beijing Forestry University, Beijing 100083, China. FAU - Wang, Yiwen AU - Wang Y AD - Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China. AD - Sino-France Joint Laboratory for Invasive Forest Pests in Eurasia, Beijing Forestry University, Beijing 100083, China. FAU - Wu, Zhuojin AU - Wu Z AD - Inner Mongolia Forestry Research Institute, Hohhot 010011, China. FAU - Liang, Te AU - Liang T AD - Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China. AD - Sino-France Joint Laboratory for Invasive Forest Pests in Eurasia, Beijing Forestry University, Beijing 100083, China. FAU - Shi, Juan AU - Shi J AD - Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China. AD - Sino-France Joint Laboratory for Invasive Forest Pests in Eurasia, Beijing Forestry University, Beijing 100083, China. LA - eng GR - 2023YFE0116200/National Key Research and Development Program of China/ GR - 32471870/National Natural Science Foundation of China/ GR - QNTD202510/the Fundamental Research Funds for the Central Universities/ GR - 202510022170/the Beijing Forestry University Undergraduate Training Programs for Innovation and Entrepreneurship/ PT - Journal Article DEP - 20260928 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705476 OTO - NOTNLM OT - MaxEnt OT - Pissodes castaneus OT - climate change OT - niche dynamics analysis OT - potential distribution COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/28 CRDT- 2026/10/27 10:37 PHST- 2026/08/13 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - insects17101002 [pii] AID - insects-17-01002 [pii] AID - 10.3390/insects17101002 [doi] PST - epublish SO - Insects. 2026 Sep 28;17(10):1002. doi: 10.3390/insects17101002. PMID- 42940912 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Sep 28 TI - Dolycoris baccarum (L.) (Hemiptera: Pentatomidae) Infestation Induced Differential Changes in Plant Physiology: Preference and Damaging Nature to Different Sesame (Sesamum indicum L.) Varieties. LID - 10.3390/insects17101001 [doi] LID - 1001 AB - Dolycoris baccarum (Hemiptera: Pentatomidae) is a cosmopolitan stink bug that causes substantial damage to several leguminous crops, including sesame. Herbivorous insect feeding can alter key physiological processes in plants, particularly photosynthesis and chlorophyll fluorescence, thereby affecting plant growth and productivity. This study investigated the effects of D. baccarum feeding on photosynthetic traits, including carbon assimilation rate (A) and stomatal conductance to water vapor (g(sw)), as well as chlorophyll fluorescence signal in different sesame varieties using a photosynthesis system and a portable gas exchange and fluorescence analyzer. Preference of D. baccarum among sesame varieties was evaluated through a choice test using seed pods, and feeding damage was quantified using the acid fuchsin test. Feeding by D. baccarum significantly reduced photosynthetic performance and chlorophyll fluorescence signal in sesame plants. The insect exhibited clear varietal preferences, and feeding damage differed significantly among sesame varieties. A positive association was observed between feeding activity and damage intensity, whereas photosynthetic parameters (A and g(sw), and chlorophyll fluorescence signal) showed negative associations with yield following insect infestation. These findings indicate that D. baccarum feeding adversely affects both the physiological performance and productivity of sesame and highlight the potential for identifying resistant or tolerant sesame varieties for breeding programs. Further studies should examine the physical characteristics and nutrient composition of sesame seed pods and seeds to better understand the host selection behavior of D. baccarum and support the development of sesame varieties with improved resistance or tolerance to stink bug infestation. FAU - Hong, Seo Yeon AU - Hong SY AD - Smart Agricultural Technology Division, Department of Upland Crop Research & Development, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Republic of Korea. FAU - Maharjan, Rameswor AU - Maharjan R AD - Smart Agricultural Technology Division, Department of Upland Crop Research & Development, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Republic of Korea. FAU - Jeon, Jun Hyoung AU - Jeon JH AD - Smart Agricultural Technology Division, Department of Upland Crop Research & Development, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Republic of Korea. FAU - Yi, Hwi Jong AU - Yi HJ AD - Smart Agricultural Technology Division, Department of Upland Crop Research & Development, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Republic of Korea. FAU - Yoon, Young Nam AU - Yoon YN AD - Smart Agricultural Technology Division, Department of Upland Crop Research & Development, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Republic of Korea. FAU - Won, Ok Jae AU - Won OJ AD - Smart Agricultural Technology Division, Department of Upland Crop Research & Development, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Republic of Korea. FAU - Guta, Rahel Dinsa AU - Guta RD AUID- ORCID: 0009-0006-2610-8732 AD - Smart Agricultural Technology Division, Department of Upland Crop Research & Development, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Republic of Korea. FAU - Lee, Jeong Eun AU - Lee JE AD - Upland Crop Breeding Division, Department of Upland Crop Sciences, National Institute of Crop & Food Science, Rural Development Administration, Miryang 50424, Republic of Korea. FAU - Kim, Sung Up AU - Kim SU AD - Upland Crop Breeding Division, Department of Upland Crop Sciences, National Institute of Crop & Food Science, Rural Development Administration, Miryang 50424, Republic of Korea. LA - eng GR - PJ01766102/Rural Development Administration/ PT - Journal Article DEP - 20260928 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705569 OTO - NOTNLM OT - acid fuchsin test OT - chlorophyll fluorescence signal OT - feeding behavior OT - management OT - photosynthesis OT - relationships OT - shield bug COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/28 CRDT- 2026/10/27 10:37 PHST- 2026/09/01 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - insects17101001 [pii] AID - insects-17-01001 [pii] AID - 10.3390/insects17101001 [doi] PST - epublish SO - Insects. 2026 Sep 28;17(10):1001. doi: 10.3390/insects17101001. PMID- 42940905 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Sep 25 TI - Low Risk of Resistance Evolution of Spodoptera frugiperda to Lufenuron and Chlorfenapyr in China. LID - 10.3390/insects17100994 [doi] LID - 994 AB - Spodoptera frugiperda is a highly destructive migratory pest that severely restricts global agricultural production. Chemical insecticides remain the primary means of field population control, yet the resistance evolution risk of S. frugiperda to two commonly applied insecticides, lufenuron and chlorfenapyr, remains poorly clarified in China. In this study, we found that the four field S. frugiperda populations collected from Jiangsu, Guangxi, Guizhou, and Yunnan Provinces exhibited susceptible or low-level resistance to chlorfenapyr, lufenuron, emamectin benzoate, and spinetoram. However, moderate resistance to chlorantraniliprole (resistance ratio > 5) was detected in the Guangxi and Guizhou populations, consistent with regional variation in diamide insecticide pressure. After 10 consecutive generations of indoor resistance screening, the selected resistant strains showed only 2.89-fold and 3.40-fold resistance increases to lufenuron and chlorfenapyr, respectively, indicating an extremely slow resistance development rate. Furthermore, the lufenuron-resistant and chlorfenapyr-resistant strains displayed negligible cross-resistance to emamectin benzoate, chlorantraniliprole, and spinetoram. Significant fitness costs were detected in both resistant strains, with marked declines in multiple growth, development and reproductive indicators. Collectively, these findings confirm that Chinese field S. frugiperda populations have a low evolutionary risk of resistance to lufenuron and chlorfenapyr, and resistance emergence is constrained by obvious fitness trade-offs. This study provides empirical evidence and theoretical guidance for the scientific formulation of S. frugiperda chemical control and resistance management strategies in China, while highlighting the need for region-specific monitoring of diamide resistance. FAU - Wang, Yunchao AU - Wang Y AD - School of Ecology, Zunyi Normal University, Zunyi 563006, China. FAU - Ma, Wenling AU - Ma W AD - Key Laboratory of Agri-Products Quality and Biosafety (Ministry of Education), Anhui Province Key Laboratory of Crop Integrated Pest Management, School of Plant Protection, Anhui Agricultural University, Hefei 230036, China. FAU - Pang, Bo AU - Pang B AD - College of Agriculture and Animal Husbandry of Xizang Autonomous Region, Lhasa 850000, China. FAU - Xiao, Zhongjiu AU - Xiao Z AD - School of Ecology, Zunyi Normal University, Zunyi 563006, China. FAU - Tang, Peizhi AU - Tang P AD - Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China. FAU - Meng, Ying AU - Meng Y AD - Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China. AD - Academy of Dongying Efficient Agricultural Technology and Industry on Saline and Alkaline Land in Collaboration with Qingdao Agricultural University, Dongying 257091, China. FAU - Zhu, Liangting AU - Zhu L AD - Nanjing Hanguang Testing Technology Co., Ltd., Nanjing 210046, China. FAU - Guo, Jiawen AU - Guo J AUID- ORCID: 0000-0001-6743-1874 AD - State Key Laboratory for Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. FAU - Yang, Kun AU - Yang K AUID- ORCID: 0000-0001-9443-6494 AD - Shandong Engineering Research Center for Environment-Friendly Agricultural Pest Management, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China. AD - Academy of Dongying Efficient Agricultural Technology and Industry on Saline and Alkaline Land in Collaboration with Qingdao Agricultural University, Dongying 257091, China. AD - Key Laboratory of Integrated Pest Management on Crops in East China, Ministry of Agriculture and Rural Affairs, Nanjing Agricultural University, No. 1 Weigang, Nanjing 210095, China. LA - eng GR - QKHQN[2025]392, QKHMS[2026]846/Guizhou Provincial Science and Technology Department/ GR - ZUNSHI BS[2023]5/Zunyi Normal University/ GR - No specific grant/project number was provided in the manuscript./Zunyi Science and Technology Association/ GR - XZ202401ZY0034/Xizang Autonomous Region Key Research and Development Program/ GR - QKHZK[2023]480/Guizhou Science and Technology Department/ GR - ZSKHZ(2025)154/Zunyi Municipal Bureau of Industry and Science and Technology/ GR - XZNKY-2026-ZF001/Xizang Academy of Agricultural and Animal Husbandry Sciences/ PT - Journal Article DEP - 20260925 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705116 OTO - NOTNLM OT - Spodoptera frugiperda OT - chemical pesticide OT - fitness cost OT - insecticide resistance COIS- The authors declare that this study received a part of funding from Hanguang Testing. The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article or the decision to submit it for publication. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/25 CRDT- 2026/10/27 10:37 PHST- 2026/07/17 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - insects17100994 [pii] AID - insects-17-00994 [pii] AID - 10.3390/insects17100994 [doi] PST - epublish SO - Insects. 2026 Sep 25;17(10):994. doi: 10.3390/insects17100994. PMID- 42940903 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Sep 25 TI - Life History of the Invasive Big-Headed Ant Pheidole megacephala (Hymenoptera: Formicidae). LID - 10.3390/insects17100992 [doi] LID - 992 AB - The African big-headed ant, Pheidole megacephala (Fabricius, 1793), ranks among the world's 100 worst invasive alien species, yet its basic life history remains poorly documented. We combined laboratory rearing and field observations in subtropical China with light and scanning electron microscopy to characterize the morphology, development, caste differentiation and reproductive behavior of this ant across its life cycle. Larvae passed through three instars. Head capsule width separated the three instars, whereas setal morphology was both instar- and caste-specific, because worker- and soldier-destined third instars remained densely setose while reproductive larvae shed their setae and were nearly glabrous as prepupae, an external marker of caste fate before pupation. At 28 degrees C, non-reproductives developed from egg to adult in 28.73 +/- 2.83 d with marked asynchrony, whereas reproductives shared the early schedule but required longer prepupal and pupal periods, totaling 37.60 +/- 3.20 d. Caste production was seasonal, colonies were polygynous, and oviposition proceeded in discrete batches. Nests were cryptic and associated with moist, sheltered microhabitats. This first integrated life-history account identifies seasonal and microhabitat vulnerabilities that surveillance and control can exploit. FAU - Yuan, Zhengying AU - Yuan Z AUID- ORCID: 0009-0001-0141-2248 AD - Guangxi Key Laboratory of Agro-Environment and Agric-Products Safety, College of Agriculture, Guangxi University, Nanning 530004, China. FAU - Nong, Ning AU - Nong N AD - Guangxi Key Laboratory of Agro-Environment and Agric-Products Safety, College of Agriculture, Guangxi University, Nanning 530004, China. FAU - Qin, Zhifang AU - Qin Z AD - Guangxi Key Laboratory of Agro-Environment and Agric-Products Safety, College of Agriculture, Guangxi University, Nanning 530004, China. FAU - Gao, Qionghua AU - Gao Q AD - Guangxi Key Laboratory of Agro-Environment and Agric-Products Safety, College of Agriculture, Guangxi University, Nanning 530004, China. LA - eng GR - 2026GXNSFAA00641062/Guangxi Natural Science Foundation/ GR - S202310593406 and 202210593041/The Innovation and Entrepreneurship Training Program for College Students of Guangxi University/ GR - 2024GXNSFBA010431/Youth Science Foundation of the Natural Science Foundation of Guangxi Province/ PT - Journal Article DEP - 20260925 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705411 OTO - NOTNLM OT - Pheidole megacephala OT - big-headed ant OT - caste differentiation OT - larval instars OT - larval setae OT - life history COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/25 CRDT- 2026/10/27 10:37 PHST- 2026/08/22 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - insects17100992 [pii] AID - insects-17-00992 [pii] AID - 10.3390/insects17100992 [doi] PST - epublish SO - Insects. 2026 Sep 25;17(10):992. doi: 10.3390/insects17100992. PMID- 42940901 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Sep 25 TI - Molecular Identification of Insects on Imperata cylindrica (Poales: Poaceae) and Exploration of Potential Natural Enemies Using DNA Barcoding. LID - 10.3390/insects17100990 [doi] LID - 990 AB - Imperata cylindrica is a highly invasive grass that causes severe ecological and agricultural damage worldwide, yet effective and sustainable management options remain limited. In this study, we investigated insects associated with the stems and rhizosphere soil of I. cylindrica in four parks in Beijing, China, and evaluated the utility of DNA barcoding for species identification and natural enemy discovery. A total of 16 insect samples were collected, and the mitochondrial COI gene was amplified using universal (LCO1490/HCO2198) and degenerate (KC-COI-F/R) primers. High-quality sequences were obtained from 12 of the 16 individual specimens, representing two classes (Insecta and Symphyla), two orders (Diptera and Coleoptera), and five families (Chloropidae, Muscidae, Rhiniidae, Eumolpidae, and Scutigerellidae). Chloropidae larvae (Diptera) dominated the stem-associated samples, and Pseudocolaspis sp. (Coleoptera: Eumolpidae), Atherigona orientalis (Diptera: Muscidae), Rhyncomya pruinosa (Diptera: Rhiniidae), and a species of Scutigerellidae (Symphyla) were also identified. BLAST analysis assigned the chloropid sequences to Chloropidae sp. BIOUG02404-H03 with 96.93-97.34% identity, and these sequences were recovered from stems at all four sampling sites. These results demonstrate that DNA barcoding effectively identifies morphologically challenging immature stages and soil-dwelling specimens. The abundant chloropid larvae, which feed internally in I. cylindrica stems, represent a promising candidate for further host-specificity testing as a classical biological control agent. This study provides the first molecular characterization of insects associated with I. cylindrica in Beijing and highlights the value of DNA barcoding in biocontrol resource discovery. FAU - Liu, Dianyu AU - Liu D AD - Sino-American Biological Control Laboratory, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China. FAU - Su, Zhihan AU - Su Z AD - Sino-American Biological Control Laboratory, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China. FAU - Xu, Wenyan AU - Xu W AD - Sino-American Biological Control Laboratory, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China. FAU - Chen, Yu AU - Chen Y AD - Sino-American Biological Control Laboratory, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China. FAU - Yan, Xiaoyu AU - Yan X AD - Sino-American Biological Control Laboratory, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China. FAU - Wang, Yichen AU - Wang Y AD - Sino-American Biological Control Laboratory, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China. FAU - Liu, Chenxi AU - Liu C AUID- ORCID: 0000-0002-1208-1309 AD - Sino-American Biological Control Laboratory, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China. FAU - Brookes, Dean AU - Brookes D AUID- ORCID: 0000-0002-6659-2956 AD - USDA ARS Australian Biocontrol Laboratory, Ecosciences Precinct, 41 Boggo Road, Dutton Park, QLD 4102, Australia. LA - eng GR - 2024082216/Commonwealth Scientific and Industrial Research Organisation/ PT - Journal Article DEP - 20260925 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13704918 OTO - NOTNLM OT - COI gene OT - Chloropidae OT - DNA barcoding OT - Imperata cylindrica OT - biological control OT - insect diversity COIS- The authors declare no conflicts of interest. The funders (USDA ARS Australian Biocontrol Laboratory) provided financial support and, through the author Dean Brookes, had a role in sample collection and manuscript review. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/25 CRDT- 2026/10/27 10:37 PHST- 2026/09/01 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - insects17100990 [pii] AID - insects-17-00990 [pii] AID - 10.3390/insects17100990 [doi] PST - epublish SO - Insects. 2026 Sep 25;17(10):990. doi: 10.3390/insects17100990. PMID- 42940900 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Sep 25 TI - Plant-Insect Interactions in Biological Invasions: From Classical Hypotheses to Multitrophic Networks and Biological Control. LID - 10.3390/insects17100989 [doi] LID - 989 AB - Biological invasions are a critical driver of global environmental change, with plant-insect interactions central to invasion success and biological control. This review systematically examines three classical hypotheses-the Enemy Release Hypothesis (ERH), the Novel Weapons Hypothesis (NWH), and the Evolution of Increased Competitive Ability (EICA) hypothesis, within a plant-insect context. Our synthesis shows that ERH support is widespread but highly contingent on environmental context; NWH highlights the negative effects of invasive allelochemicals on native insects and soil biota; and the EICA hypothesis provides an evolutionary framework for understanding shifts in defence-growth resource allocation in invasive plants. We advocate for a multitrophic network perspective that captures ecosystem complexity and elucidates synergistic interactions among hypotheses across space and time. We identify inherent limitations in single-hypothesis biocontrol approaches and propose an integrative management framework grounded in network theory. Emerging tools-including molecular ecology and network modelling-are emphasised for resolving cryptic interactions and forecasting control outcomes. Future priorities include interdisciplinary integration, long-term monitoring, global comparisons, and addressing climate-driven uncertainties. This review aims to advance both theoretical invasion ecology and the development of sustainable, evidence-based biological control strategies. FAU - Li, Qinchun AU - Li Q AD - College of Civil and Environmental Engineering, Hunan Institute of Science and Engineering, Yongzhou 425199, China. FAU - Xu, Fanping AU - Xu F AD - College of Chemistry and Bioengineering, Hunan Institute of Science and Engineering, Yongzhou 425199, China. FAU - Cheng, Wenzhi AU - Cheng W AD - School of Information Engineering, Hunan Institute of Science and Engineering, Yongzhou 425199, China. FAU - Qi, Chengmei AU - Qi C AD - College of Chemistry and Bioengineering, Hunan Institute of Science and Engineering, Yongzhou 425199, China. FAU - Liu, Xiaowen AU - Liu X AD - College of Chemistry and Bioengineering, Hunan Institute of Science and Engineering, Yongzhou 425199, China. LA - eng GR - 24B0755/Education Department of Hunan Province/ GR - Natural Science Foundation of Hunan Province/Natural Science Foundation of Hunan Province/ PT - Journal Article PT - Review DEP - 20260925 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705013 OTO - NOTNLM OT - enemy release hypothesis OT - evolution of increased competitive ability OT - food web OT - novel weapons hypothesis OT - pest management OT - trophic cascade COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/25 CRDT- 2026/10/27 10:37 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - insects17100989 [pii] AID - insects-17-00989 [pii] AID - 10.3390/insects17100989 [doi] PST - epublish SO - Insects. 2026 Sep 25;17(10):989. doi: 10.3390/insects17100989. PMID- 42940899 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Sep 24 TI - Soil-Arthropod Interactions Across Forest Types in a Mediterranean Mountain Landscape. LID - 10.3390/insects17100988 [doi] LID - 988 AB - Ground-dwelling arthropods contribute to decomposition, nutrient cycling, and biological control, but their relationships with soil properties remain insufficiently understood in Mediterranean mountain forests. We evaluated taxonomic and functional structure, seasonal variation, and associations with edaphic gradients across three forest stands-one holm oak (Quercus rotundifolia), one Pyrenean oak (Quercus pyrenaica), and one maritime pine (Pinus pinaster)-in Montesinho Natural Park, northeastern Portugal. Arthropods were sampled with pitfall traps during four campaigns from July 2023 to April 2024, and soil properties were characterized at each trap. We recorded 5511 arthropods, comprising 18 orders, 54 taxa, and six functional groups. Total abundance was lowest in February, highest in April, and lower in the Pyrenean oak stand than in the other stands. Taxon richness, predator abundance, and the abundance of taxa with unresolved or multiple trophic functions varied differently among stands across campaigns. The sampling campaign explained 28.7% of the variation in taxonomic composition. Total abundance and omnivore abundance, the latter dominated by Formicidae, increased along PC1, associated with finer-textured soils, higher pH and C:N ratio, and lower available potassium. Omnivores decreased along PC2, associated mainly with organic matter, roots, and clay-bound nitrogen. PC1 and PC2 jointly explained 33.2% of the variation in taxonomic composition. Overall, the results show that seasonal and edaphic variation are important in structuring the taxonomic and functional components of ground-dwelling arthropod communities in Mediterranean mountain forests. FAU - Villa, Maria AU - Villa M AUID- ORCID: 0000-0001-8353-5438 AD - CIMO, LA SusTEC, Universidade Politecnica de Braganca, Campus de Santa Apolonia, 5300-253 Braganca, Portugal. FAU - Angelico, Diogo AU - Angelico D AUID- ORCID: 0009-0007-1582-0457 AD - CIMO, LA SusTEC, Universidade Politecnica de Braganca, Campus de Santa Apolonia, 5300-253 Braganca, Portugal. FAU - Castro, Marina AU - Castro M AUID- ORCID: 0000-0002-6368-8098 AD - CIMO, LA SusTEC, Universidade Politecnica de Braganca, Campus de Santa Apolonia, 5300-253 Braganca, Portugal. LA - eng GR - Project 10107445-LIFE21-CCA-ESLIFE SILFORE/European Commission/ GR - CIMO, UID/00690/2025 (DOI: 10.54499/UID/00690/2025) and UID/PRR/00690/2025 (DOI: (10.54499/UID/PRR/00690/2025); and SusTEC, LA/P/0007/2020 (DOI: 10.54499/LA/P/0007/2020)./Fundacao para a Ciencia e Tecnologia/ PT - Journal Article DEP - 20260924 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705440 OTO - NOTNLM OT - Mediterranean mountain forests OT - edaphic gradients OT - functional groups OT - ground-dwelling arthropods OT - seasonal variation OT - taxonomic composition COIS- Maria Villa is the Guest Editor of the Special Issue to which this manuscript is submitted and was not involved in the editorial handling, reviewer selection, or decision-making process for this manuscript. The other authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:37 PHST- 2026/08/16 00:00 [received] PHST- 2026/09/08 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - insects17100988 [pii] AID - insects-17-00988 [pii] AID - 10.3390/insects17100988 [doi] PST - epublish SO - Insects. 2026 Sep 24;17(10):988. doi: 10.3390/insects17100988. PMID- 42940889 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2075-4450 (Print) IS - 2075-4450 (Electronic) IS - 2075-4450 (Linking) VI - 17 IP - 10 DP - 2026 Sep 22 TI - Variation in Hygienic Behavior and Its Associations with Colony-Performance and Wing Morphometric Traits Among Honey Bee Colonies from Five Apiaries in Kazakhstan. LID - 10.3390/insects17100978 [doi] LID - 978 AB - Honey bee hygienic behavior is an important colony-level defense trait with potential relevance to selective breeding. The aim of this study was to evaluate variation in hygienic behavior among honey bee colonies from five apiaries in Kazakhstan and to examine its associations with colony-performance traits and wing morphometric characteristics. Hygienic behavior was assessed in 50 colonies using the pin-killed brood assay, with brood removal recorded 24 h after treatment. Significant differences in hygienic behavior were observed among the five studied apiaries (p < 0.001), with the highest mean brood-removal efficiencies recorded in the Nurik and KazNARU apiaries and the lowest in the Baizhigitov apiary. Principal component and hierarchical clustering analyses revealed multivariate differentiation among colonies based on behavioral, performance, and morphometric characteristics. Although the cubital index was strongly correlated with hygienic behavior in the pooled analysis, the within-apiary association was not statistically significant after accounting for apiary identity (beta = 9.42, 95% CI: -2.82 to 21.65, p = 0.128), indicating that the overall relationship was primarily attributable to differences among apiaries. However, because apiary identity is associated with ecological conditions as well as potentially differing management practices and honey bee population backgrounds, these patterns should not be interpreted as independent causal effects of ecological conditions. The findings provide baseline information on variation in hygienic behavior and associated phenotypic traits in honey bee colonies from Kazakhstan and support further studies incorporating broader environmental, management, and genetic characterization before their potential application in selective breeding programs. FAU - Nuraliyeva, Ulzhan AU - Nuraliyeva U AD - Kazakh Research Institute of Livestock and Fodder Production, Almaty 050035, Kazakhstan. AD - Zooengineering and Biotechnology Department, Kazakh National Agrarian Research University, Almaty 050010, Kazakhstan. FAU - Moldakhmetova, Gaukhar AU - Moldakhmetova G AUID- ORCID: 0000-0002-1860-4721 AD - Kazakh Research Institute of Livestock and Fodder Production, Almaty 050035, Kazakhstan. FAU - Khalykova, Gulim AU - Khalykova G AD - Kazakh Research Institute of Livestock and Fodder Production, Almaty 050035, Kazakhstan. FAU - Torekhanov, Merey AU - Torekhanov M AD - Institute of Genetics and Physiology, 93 Al-Farabi Avenue, Almaty 050060, Kazakhstan. FAU - Shokan, Aksholpan AU - Shokan A AD - Institute of Genetics and Physiology, 93 Al-Farabi Avenue, Almaty 050060, Kazakhstan. FAU - Taufikh, Akbota AU - Taufikh A AD - Kazakh Research Institute of Livestock and Fodder Production, Almaty 050035, Kazakhstan. FAU - Toishimanov, Maxat AU - Toishimanov M AUID- ORCID: 0000-0002-6070-4574 AD - Food and Environmental Safety Laboratory, Kazakh National Agrarian Research University, Almaty 050010, Kazakhstan. AD - Department of Food Safety and Quality, Faculty of Food Technology, Almaty Technological University, Almaty 050012, Kazakhstan. LA - eng GR - AP23490810/Ministry of Science and Higher Education of the Republic of Kazakhstan/ PT - Journal Article DEP - 20260922 PL - Switzerland TA - Insects JT - Insects JID - 101574235 PMC - PMC13705145 OTO - NOTNLM OT - Apis mellifera OT - colony performance OT - cubital index OT - honey bee breeding OT - hygienic behavior OT - morphometry OT - pin-killed brood assay COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:37 PHST- 2026/07/21 00:00 [received] PHST- 2026/09/05 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - insects17100978 [pii] AID - insects-17-00978 [pii] AID - 10.3390/insects17100978 [doi] PST - epublish SO - Insects. 2026 Sep 22;17(10):978. doi: 10.3390/insects17100978. PMID- 42940882 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 21 TI - Correspondence Principle Approach to Time-Dependent Decaying Quantized Fields. LID - 10.3390/e28101160 [doi] LID - 1160 AB - We present a correspondence principle framework that maps a classical field-amplitude decay law Gamma(t) onto a vacuum-loss quantum master equation for a single bosonic mode. Under the strict assumption that an initially coherent state remains pure, the ansatz selects a vacuum reservoir loss model rather than fixing general open-system quantum dynamics. While classical attenuation alone does not fix the noise structure accordingly, the derived generator is a compatible quantum model under explicit zero-temperature assumptions, not a consequence of the classical envelope alone-as thermal noise or environmental fluctuations can produce different quantum states with identical mean amplitudes. After reviewing representative classical regimes we derive a generalized master equation for a single bosonic mode whose complex amplitude is alphaGamma(t), where Gamma(t) is a real, positive, strictly monotonic envelope. The resulting dynamics reduces to the standard amplitude-damping Lindblad equation for Gamma(t)=exp(-gammat/2) and preserves the coherent-state form |alphaGamma(t)⟩⟨alphaGamma(t)|. We clarify the exact physical assumptions required for this construction and distinguish CP-divisibility from non-Markovian memory effects. We prove uniqueness of this generator only within the stated pure-loss, single-mode, real-envelope assumptions, clarify its exact equivalence to a time-reparametrized pure-loss channel and discuss the limits of validity in dispersive absorbing media within macroscopic QED. FAU - Ramos-Prieto, Iran AU - Ramos-Prieto I AUID- ORCID: 0000-0001-8838-3541 AD - Instituto Nacional de Astrofisica Optica y Electronica, Calle Luis Enrique Erro No. 1, Santa Maria Tonantzintla 72840, Puebla, Mexico. FAU - Korneev, Nikolai AU - Korneev N AUID- ORCID: 0000-0001-8405-1195 AD - Instituto Nacional de Astrofisica Optica y Electronica, Calle Luis Enrique Erro No. 1, Santa Maria Tonantzintla 72840, Puebla, Mexico. FAU - Moya-Cessa, Hector M AU - Moya-Cessa HM AUID- ORCID: 0000-0003-1444-0261 AD - Instituto Nacional de Astrofisica Optica y Electronica, Calle Luis Enrique Erro No. 1, Santa Maria Tonantzintla 72840, Puebla, Mexico. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13703370 OTO - NOTNLM OT - correspondence principle OT - lindblad operators OT - master equation OT - time-dependent fields COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:37 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - e28101160 [pii] AID - entropy-28-01160 [pii] AID - 10.3390/e28101160 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 21;28(10):1160. doi: 10.3390/e28101160. PMID- 42940878 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 20 TI - A Data Compression Method for Mechanical Vibration Wireless Sensor Networks Based on Data Segmentation and Non-Uniform Quantization. LID - 10.3390/e28101156 [doi] LID - 1156 AB - High-frequency sampling in mechanical vibration wireless sensor networks (WSNs) generates large volumes of raw data, imposing substantial demands on transmission bandwidth and node energy consumption. To address this problem, this paper proposes a two-stage compression method based on data segmentation and non-uniform quantization. First, byte-level statistical characteristics of 24-bit raw analog-to-digital converter (ADC) data are analyzed. The results reveal the limitations of conventional transform-domain thresholding and show distinct statistical characteristics among different bit planes. Accordingly, each 24-bit sample is divided into an 8-bit sign-bit channel and a 16-bit content-bit channel. The sign-bit channel exhibits high temporal redundancy and is therefore compressed losslessly using variable run-length encoding (VRLE). An adaptive repetition-ratio criterion is introduced to select the encoding mode and avoid data expansion for short repetition sequences. The content-bit channel is compressed using an eight-interval non-uniform quantization scheme inspired by the A-Law PCM standard. Finer quantization is assigned to small-amplitude components to reduce reconstruction errors. Range coding is subsequently applied to both encoded streams to further remove statistical redundancy. Experiments on periodic rotor vibration signals achieve a compression ratio of 3.22 and a reconstruction signal-to-noise ratio (RSNR) of 37.4 dB. For aperiodic modal testing signals, compression ratios of 3.16 and 2.66 are obtained for force-hammer excitation and frame response signals, with RSNR values of 46.49 and 54.2 dB, respectively. Furthermore, modal identification using the reconstructed data results in a maximum relative error of only 0.03% for the first five natural frequencies. These results demonstrate that the proposed method effectively reduces vibration data volume while preserving critical dynamic characteristics. FAU - Huang, Yi AU - Huang Y AUID- ORCID: 0000-0002-7494-4771 AD - School of Intelligent Manufacturing, Chongqing University of Arts and Sciences, Chongqing 402160, China. AD - Key Laboratory of Optoelectronic Technology & Systems Ministry of Education, International R & D Center of Micro-Nano Systems and New Materials Technology, Chongqing University, Chongqing 400044, China. FAU - Zhang, Jiaquan AU - Zhang J AD - School of Intelligent Manufacturing, Chongqing University of Arts and Sciences, Chongqing 402160, China. FAU - Mu, Xiaojing AU - Mu X AD - Key Laboratory of Optoelectronic Technology & Systems Ministry of Education, International R & D Center of Micro-Nano Systems and New Materials Technology, Chongqing University, Chongqing 400044, China. FAU - Tian, Dongmei AU - Tian D AD - School of Intelligent Manufacturing, Chongqing University of Arts and Sciences, Chongqing 402160, China. FAU - Mu, Rongtao AU - Mu R AD - School of Intelligent Manufacturing, Chongqing University of Arts and Sciences, Chongqing 402160, China. FAU - Luo, Tianhong AU - Luo T AD - School of Intelligent Manufacturing, Chongqing University of Arts and Sciences, Chongqing 402160, China. AD - Department of Information and Intelligence Engineering, Chongqing City Vocational College, Chongqing 402160, China. LA - eng GR - 52405580/National Natural Science Foundation of China/ GR - KJQN202501303/Chongqing Municipality Education Commission/ GR - KJQN202401359/Chongqing Municipality Education Commission/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13702937 OTO - NOTNLM OT - data compression OT - data segmentation OT - mechanical vibration monitoring OT - non-uniform quantization OT - range coding OT - variable run-length encoding OT - wireless sensor networks COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:37 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - e28101156 [pii] AID - entropy-28-01156 [pii] AID - 10.3390/e28101156 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 20;28(10):1156. doi: 10.3390/e28101156. PMID- 42940877 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 20 TI - Iterative Screening Density Peak Clustering. LID - 10.3390/e28101155 [doi] LID - 1155 AB - Density peak clustering (DPC) is popular in unsupervised clustering due to its intuitive theory and the absence of pre-defined cluster numbers. However, traditional DPC relies on a static single computation strategy. It uses all raw samples to calculate global density and relative distance without iterative correction. Low-density samples in real data distort the density distribution, weaken the peak characteristics of real clustering centers, lead to incorrect center selection and disordered point allocation, and damage the stability and accuracy of clustering. To address this limitation, we propose an Iterative Screening Density Peak Clustering (IS-DPC). A new local density based on binary selection is designed to suppress interference from low-density samples at the computational source. In addition, the iterative mechanism transforms static single-round calculations into dynamic progressive updates. Experiments on real datasets have shown that IS-DPC outperforms several advanced DPC variants in most cases. FAU - Deng, Zilong AU - Deng Z AUID- ORCID: 0009-0009-8196-9481 AD - Centre for Image and Vision Computing, CoE for Artificial Intelligence, Faculty of Computing and Informatics, Multimedia University, Cyberjaya 63100, Selangor, Malaysia. AD - College of Information Technology, Anqing Vocational and Technical College, Anqing 246003, China. FAU - Alobaedy, Mustafa Muwafak AU - Alobaedy MM AUID- ORCID: 0000-0002-6562-2922 AD - Centre for Image and Vision Computing, CoE for Artificial Intelligence, Faculty of Computing and Informatics, Multimedia University, Cyberjaya 63100, Selangor, Malaysia. FAU - Kamal, Shahid AU - Kamal S AUID- ORCID: 0009-0009-7995-175X AD - Center for Advanced Analytics, CoE for Artificial Intelligence, Faculty of Computing & Informatics, Multimedia University, Cyberjaya 63100, Selangor, Malaysia. FAU - Cheng, Fang AU - Cheng F AD - College of Information Technology, Anqing Vocational and Technical College, Anqing 246003, China. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13703416 OTO - NOTNLM OT - density peak OT - image clustering OT - screening COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:37 PHST- 2026/08/13 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - e28101155 [pii] AID - entropy-28-01155 [pii] AID - 10.3390/e28101155 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 20;28(10):1155. doi: 10.3390/e28101155. PMID- 42940872 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 20 TI - Dual-View Complementary Multiscale Entropy Fusion for Rotating-Machinery Fault Diagnosis. LID - 10.3390/e28101150 [doi] LID - 1150 AB - Entropy-based fault diagnosis depends on signal representation and the properties captured by each entropy measure. We evaluated dual-view complementary multiscale entropy fusion (DCMEF), which combines RMS-normalized raw vibration with a normalized logarithmic Hilbert envelope. Three entropy measures at five fixed scales produce 30 candidate features, with imputation, selection, and classifier tuning confined to grouped training folds. In an exploratory comparison against 20 representations across four selected tasks and three classifiers, DCMEF achieved the highest observed accuracy in nine of 12 settings and tied for highest in three. A separate view ablation found that the full bank outperformed raw-only features in two settings, tied in five, and underperformed in five; relative to envelope-only, it outperformed in ten and tied in two. Thus, raw-vibration features were useful across these settings, whereas adding envelope features had inconsistent effects. Independent evaluation is needed to assess performance beyond the selected tasks. FAU - Xiao, Wei AU - Xiao W AD - Business School, Hohai University, Nanjing 211100, China. FAU - Zhang, Yang AU - Zhang Y AD - School of Automation, Xi'an University of Posts and Telecommunications, Xi'an 710121, China. FAU - Xu, Rui AU - Xu R AD - Business School, Hohai University, Nanjing 211100, China. LA - eng GR - B250207085/Fundamental Research Funds for the Central Universities/ GR - 26GLC029/Jiangsu Provincial Social Science Fund/ GR - 2024SJYB0054/Philosophy and Social Science Fund of Education Department of Jiangsu Province/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13702936 OTO - NOTNLM OT - entropy features OT - fault diagnosis OT - feature fusion OT - grouped cross-validation OT - multiscale entropy OT - rotating machinery OT - vibration analysis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:37 PHST- 2026/09/25 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - e28101150 [pii] AID - entropy-28-01150 [pii] AID - 10.3390/e28101150 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 20;28(10):1150. doi: 10.3390/e28101150. PMID- 42940869 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 18 TI - Proof-of-Principle Implementation of Quantum Key Distribution in an Indefinite Causal Order. LID - 10.3390/e28101147 [doi] LID - 1147 AB - In quantum physics, the order in which different operations occur can be placed in superposition. The resulting processes have an indefinite causal order which is of fundamental interest and can be viewed as a novel quantum resource enabling new protocols. Here, we report an experimental proof-of-principle implementation of one such protocol, where we perform BB84-like quantum cryptography by placing Alice and Bob's measurement-and-preparation operations in a photonic quantum SWITCH. By embedding Alice and Bob within the quantum SWITCH, the protocol achieves an average eavesdropper detection probability of 0.15 (0.02) per shared qubit, with eavesdropper detection performed through measurements of the control qubit rather than comparing the key. Unlike standard BB84 and related schemes, which detect eavesdropping by publicly revealing and discarding a fraction of the raw key, our approach requires no disclosure of key material: every retained qubit can, in principle, be tested for eavesdropping while remaining available for key generation. Although the present implementation does not yet constitute a secure quantum key distribution protocol, owing to post-selection required for measurements within the quantum SWITCH, it provides a proof of principle that indefinite causal order can be exploited to detect eavesdropping without sacrificing key bits. FAU - Valibouse, Yann AU - Valibouse Y AUID- ORCID: 0009-0000-9622-1972 AD - Vienna Center for Quantum Science and Technology (VCQ), Faculty of Physics, University of Vienna, Boltzmanngasse 5, 1090 Vienna, Austria. AD - Vienna Doctoral School in Physics, University of Vienna, Boltzmanngasse 5, 1090 Vienna, Austria. FAU - Cladera-Rossello, Marti AU - Cladera-Rossello M AUID- ORCID: 0009-0002-6755-9266 AD - Vienna Center for Quantum Science and Technology (VCQ), Faculty of Physics, University of Vienna, Boltzmanngasse 5, 1090 Vienna, Austria. AD - Vienna Doctoral School in Physics, University of Vienna, Boltzmanngasse 5, 1090 Vienna, Austria. FAU - Antesberger, Michael AU - Antesberger M AD - Vienna Center for Quantum Science and Technology (VCQ), Faculty of Physics, University of Vienna, Boltzmanngasse 5, 1090 Vienna, Austria. AD - Vienna Doctoral School in Physics, University of Vienna, Boltzmanngasse 5, 1090 Vienna, Austria. FAU - Spencer-Wood, Hector AU - Spencer-Wood H AUID- ORCID: 0000-0002-2737-1640 AD - School of Physics and Astronomy, University of Glasgow, Glasgow G12 8QQ, UK. FAU - Simonov, Kyrylo AU - Simonov K AUID- ORCID: 0000-0001-6764-8555 AD - Fakultat fur Mathematik, Universitat Wien, Oskar-Morgenstern-Platz 1, 1090 Vienna, Austria. FAU - Sund, Patrik I AU - Sund PI AUID- ORCID: 0000-0001-7402-1216 AD - Vienna Center for Quantum Science and Technology (VCQ), Faculty of Physics, University of Vienna, Boltzmanngasse 5, 1090 Vienna, Austria. FAU - Bozzio, Mathieu AU - Bozzio M AUID- ORCID: 0000-0003-3661-2810 AD - Vienna Center for Quantum Science and Technology (VCQ), Faculty of Physics, University of Vienna, Boltzmanngasse 5, 1090 Vienna, Austria. FAU - Walther, Philip AU - Walther P AUID- ORCID: 0000-0002-4964-817X AD - Vienna Center for Quantum Science and Technology (VCQ), Faculty of Physics, University of Vienna, Boltzmanngasse 5, 1090 Vienna, Austria. AD - Christian Doppler Laboratory for Photonic Quantum Computer, Boltzmanngasse 5, 1090 Vienna, Austria. AD - Institute for Quantum Optics and Quantum Information (IQOQI) Vienna, Austrian Academy of Sciences, Boltzmanngasse 3, 1090 Vienna, Austria. FAU - Rozema, Lee A AU - Rozema LA AUID- ORCID: 0000-0003-4580-7871 AD - Vienna Center for Quantum Science and Technology (VCQ), Faculty of Physics, University of Vienna, Boltzmanngasse 5, 1090 Vienna, Austria. LA - eng GR - 101071779/ERC_/European Research Council/International GR - 899368/European Union/ GR - 956071/European Union/ GR - 101135288/European Union/ GR - 10.55776/COE1/FWF Austrian Science Fund/ GR - 10.55776/F71/FWF Austrian Science Fund/ GR - 10.55776/FG5/FWF Austrian Science Fund/ GR - 10.55776/PAT4559623/FWF Austrian Science Fund/ GR - FA9550-21-1-0355/United States Air Force Office of Scientific Research/ GR - FA8655-23-1-7063/United States Air Force Office of Scientific Research/ GR - EP/W026910/1/Engineering and Physical Sciences Research Council/ GR - EP/Y014456/1/Engineering and Physical Sciences Research Council/ PT - Journal Article DEP - 20261018 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13703698 OTO - NOTNLM OT - BB84 OT - indefinite causal order OT - photonic quantum information OT - quantum SWITCH OT - quantum key distribution COIS- The authors declare no conflicts of interest. The sponsors had no role in the design, execution, interpretation, or writing of the study. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:37 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - e28101147 [pii] AID - entropy-28-01147 [pii] AID - 10.3390/e28101147 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 18;28(10):1147. doi: 10.3390/e28101147. PMID- 42940863 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 17 TI - Visibility-Graph-Based Relative Strength Indicator for Financial Market Decision-Making. LID - 10.3390/e28101141 [doi] LID - 1141 AB - This study introduces the Visibility-Graph-Based Relative Strength Index (VGRSI), a causal indicator that selects and implicitly weights price increments using backward natural visibility. Independent parameters specify the aggregation window and the backward visibility range. Two aggregation variants use the magnitudes and counts of positive and negative contributions to produce values on a 0-100 scale. The VGRSI was tested in an automated walk-forward experiment based on 30-day optimisation windows followed by 7-day walk-forward windows. This study covered DJI30, EUR/USD, and XAU/USD over 503 trading days in 2024-2025. The VGRSI strategy produced positive cumulative net profits for all three instruments, whereas the tested classical-RSI strategy and the VGRSI variant without visibility selection produced cumulative losses under the same walk-forward procedure and trading conditions. Because the analysis covered only three instruments and one historical period, the results should be treated as preliminary. FAU - Rak, Rafal AU - Rak R AUID- ORCID: 0000-0001-9780-4205 AD - Institute of Physics, Faculty of Exact and Technical Sciences, University of Rzeszow, Pigonia 1, 35-310 Rzeszow, Poland. LA - eng PT - Journal Article DEP - 20261017 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13703518 OTO - NOTNLM OT - algorithmic trading OT - complex networks OT - financial decision-making OT - natural visibility graph OT - relative strength index OT - visibility graphs OT - walk-forward evaluation COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:37 PHST- 2026/09/30 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - e28101141 [pii] AID - entropy-28-01141 [pii] AID - 10.3390/e28101141 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 17;28(10):1141. doi: 10.3390/e28101141. PMID- 42940860 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 16 TI - Coupled Spreading Dynamics of Competitive Information and Epidemics on Weighted Multilayer Networks with Higher-Order Interactions. LID - 10.3390/e28101138 [doi] LID - 1138 AB - The spread of epidemics in real social systems is often coupled with competitive information diffusion, higher-order group interactions, and dynamic contact behaviors. To capture these mechanisms, we develop a bilayer competitive information-epidemic coupled spreading framework. The upper layer is a weighted higher-order network incorporating both pairwise spreading and 2-simplex-driven group spreading, while the lower layer is a weighted activity-driven network describing time-varying physical contacts regulated by a policy relaxation parameter. Based on the microscopic Markov chain framework, we establish node-level evolution equations and derive a spectral condition for the local invasion threshold, which is evaluated numerically. Monte Carlo simulations show good agreement with the predicted steady-state densities. These findings indicate that enhancing the diffusion of the more protective information reduces steady-state epidemic prevalence and raises the epidemic threshold, whereas preference for the less protective information weakens the overall protective effect. Higher-order interactions exert asymmetric effects on competing information, while social tie weights and activity-driven contacts also significantly influence epidemic spreading. Moreover, the epidemic threshold is jointly affected by recovery probability, activity heterogeneity, information-layer topology, and policy regulation. These findings reveal the cross-layer coupling between higher-order information diffusion and temporal physical contacts. FAU - Yin, Qian AU - Yin Q AD - School of Electronics and Information Engineering, Wuyi University, Jiangmen 529020, China. FAU - Li, Haoxian AU - Li H AD - School of Electronics and Information Engineering, Wuyi University, Jiangmen 529020, China. FAU - Wang, Zhishuang AU - Wang Z AD - School of Electronics and Information Engineering, Wuyi University, Jiangmen 529020, China. FAU - Wang, Kaiyao AU - Wang K AD - School of Electronics and Information Engineering, Wuyi University, Jiangmen 529020, China. FAU - Li, Jingmin AU - Li J AD - School of Electronics and Information Engineering, Wuyi University, Jiangmen 529020, China. FAU - Hong, Zhiyong AU - Hong Z AD - School of Electronics and Information Engineering, Wuyi University, Jiangmen 529020, China. LA - eng GR - 62503372/National Natural Science Foundation of China/ GR - 2023A1515110305/Guangdong Basic and Applied Basic Research Foundation/ GR - 2025A1515110184/Guangdong Basic and Applied Basic Research Foundation/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13703884 OTO - NOTNLM OT - activity-driven networks OT - coupled spreading OT - higher-order interactions OT - multilayer complex networks COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:37 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - e28101138 [pii] AID - entropy-28-01138 [pii] AID - 10.3390/e28101138 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 16;28(10):1138. doi: 10.3390/e28101138. PMID- 42940857 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 15 TI - Resilience Phase Transitions in Networks with Heterogeneous Node Growth Rules. LID - 10.3390/e28101135 [doi] LID - 1135 AB - Network growth and node-removal robustness are often compared while node arrivals and link budgets also vary. We hold these two quantities fixed using the email-Eu-core and CollegeMsg temporal communication networks and vary only the selection of older attachment targets. Uniform, degree-only, activity-only, combined degree-activity, and hybrid kernels are evaluated with 30 independent graph realizations per setting. Random failures use 50 deletion orders per graph; intentional attacks use static degree, exact static betweenness, and degree recalculated after every deletion. Uncertainty is summarized by realization-level 95% confidence intervals. Under static degree attack, the mean integrated robustness falls from 0.456 to 0.313 for email-Eu-core and from 0.388 to 0.176 for CollegeMsg when uniform attachment is replaced by the combined kernel. Both single-factor kernels are less damaging than their combination, and the ranking of the five counterfactual rules is unchanged under adaptive degree removal. The combined kernel has higher intact-network global efficiency but lower random-failure robustness than uniform attachment in both data sets; no universal reversal between random and intentional attacks is observed. Susceptibility peaks are finite-network, grid-dependent fragmentation indicators, not estimates of thermodynamic critical points. The results identify how retrospective target-selection counterfactuals redistribute structural vulnerability and separate this effect from dynamical recovery. FAU - He, Wanjing AU - He W AUID- ORCID: 0009-0000-2183-7182 AD - School of Intelligent Engineering and Automation, Beijing University of Posts and Telecommunications, Beijing 100876, China. FAU - Zhuang, Yufeng AU - Zhuang Y AUID- ORCID: 0009-0007-9903-687X AD - School of Intelligent Engineering and Automation, Beijing University of Posts and Telecommunications, Beijing 100876, China. FAU - Hu, Yanzhu AU - Hu Y AUID- ORCID: 0000-0002-0115-3143 AD - School of Intelligent Engineering and Automation, Beijing University of Posts and Telecommunications, Beijing 100876, China. LA - eng GR - 2024YFC3016003/the National Key R&D Program of China/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13703465 OTO - NOTNLM OT - complex networks OT - degree entropy OT - heterogeneous attachment OT - network fragmentation OT - structural robustness COIS- The authors declare no conflicts of interest. The funder had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:37 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - e28101135 [pii] AID - entropy-28-01135 [pii] AID - 10.3390/e28101135 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 15;28(10):1135. doi: 10.3390/e28101135. PMID- 42940854 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 15 TI - Entropy Regularization in the Mean-Field Free-Energy Machine for Combinatorial Optimization. LID - 10.3390/e28101132 [doi] LID - 1132 AB - The free-energy machine (FEM) relaxes discrete combinatorial optimization by minimizing expected energy plus a temperature-dependent negative-entropy penalty over product distributions. This contribution investigates the computational and mathematical role of that entropy term. At high temperature, entropy drives the optimizer toward the uniform product law; explicit relative-entropy and total-variation bounds quantify this effect, while an influence condition and a restricted Hessian characterize uniqueness, contraction, and the first possible loss of stability. As temperature decreases, the entropy coefficient vanishes. Uniform and variational convergence then connect free energy to the original discrete objective, obtaining an inverse-temperature excess-energy bound, ground-state concentration, and entropy selection among degenerate optima. Extraction bounds, path tracking, perturbation stability, and factorwise complexity further distinguish the effects of entropy, annealing, and replica selection. A batched annealed FEM algorithm is evaluated on exactly enumerable Ising, MaxCut, and Max-3SAT instances; the results reveal the predicted entropy-to-energy transition and show problem-dependent benefits of annealing and replication. For empirical-loss energies, a PAC-Bayes inequality additionally calibrates inverse temperature to sample size. The analysis characterizes entropy-dominated, instability, and energy-dominated regimes without attributing global guarantees to the nonconvex optimizer. FAU - Gao, Wei AU - Gao W AUID- ORCID: 0009-0000-7856-9828 AD - School of Mathematics, Hohai University, Nanjing 211100, China. LA - eng GR - B250201225/Hohai University/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13703487 OTO - NOTNLM OT - annealing algorithm OT - combinatorial optimization OT - entropic regularization OT - free-energy machine OT - mean-field approximation COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:37 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - e28101132 [pii] AID - entropy-28-01132 [pii] AID - 10.3390/e28101132 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 15;28(10):1132. doi: 10.3390/e28101132. PMID- 42940851 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 14 TI - An Uncertainty-Robust Decision Framework Based on Fractional Einstein Aggregation for Prioritizing Renewable Power Generation Technologies with Unknown Weights. LID - 10.3390/e28101129 [doi] LID - 1129 AB - Renewable power generation plays a central role in the transition toward sustainable, low-carbon energy systems. However, selecting an appropriate technology remains challenging because technical, economic, environmental, and operational criteria may conflict, while expert judgments may involve uncertainty and diversity. To address these challenges, this study proposes a fractional fuzzy multi-criteria group decision-making framework for evaluating and ranking renewable power generation technologies under uncertain expert assessments. A new family of aggregation operators is developed by incorporating fractional fuzzy information into the Einstein operational framework, providing a mechanism for representing and aggregating uncertain assessments. An entropy-based weighting mechanism is further employed to determine criterion weights from the available decision information. The proposed components are integrated into a unified decision procedure that combines expert weighting, fractional fuzzy aggregation, criterion weighting, and distance-based TOPSIS assessment within a common computational framework. The numerical case study considers technical, economic, environmental, and operational criteria for renewable power generation technology selection. A sensitivity analysis examines the influence of the fractional parameters on the resulting rankings, while a comparative analysis with existing decision-making approaches is conducted to examine the consistency of the obtained results. The findings demonstrate the application of the proposed framework under the specified assessment conditions and illustrate the role of fractional fuzzy information and Einstein-based aggregation in the decision process. The study provides an integrated approach for incorporating uncertain expert information into renewable energy technology selection. FAU - Albaity, Majed AU - Albaity M AUID- ORCID: 0000-0001-6599-5975 AD - Department of Mathematics, Faculty of Science, King Abdulaziz University, P.O. Box 80348, Jeddah 22254, Saudi Arabia. FAU - Rahim, Muhammad AU - Rahim M AUID- ORCID: 0000-0003-0064-0414 AD - Department of Mathematics and Statistics, University of Swat, Swat 19200, Pakistan. LA - eng GR - IPP:1588-130-2025/King Abdulaziz University/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13702905 OTO - NOTNLM OT - Einstein aggregation operators OT - TOPSIS method OT - decision making OT - fractional orthopair fuzzy sets OT - renewable power generation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:37 PHST- 2026/09/15 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - e28101129 [pii] AID - entropy-28-01129 [pii] AID - 10.3390/e28101129 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 14;28(10):1129. doi: 10.3390/e28101129. PMID- 42940848 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 14 TI - FBS26: An Entropy-Based Metric for Assessing Architectural Maturity of UML Class Diagrams. LID - 10.3390/e28101126 [doi] LID - 1126 AB - Entropy-based metrics can provide a quantitative representation of the information content of UML models, but their practical usefulness depends on a systematic and reproducible calibration of the parameters used to construct the underlying probability distribution. This paper presents FBS26, an extension of the FBS metric that introduces calibrated weights for UML element kinds while retaining the normalized Shannon entropy formulation. The proposed calibration procedure combines three criteria: avoiding false lack-of-information cases, reducing non-monotonic behavior of the metric, and achieving a pronounced decrease in the metric for balanced diagram series. The criteria are applied according to a predefined lexicographic priority. The approach is evaluated using a controlled series of UML class diagrams and 123 UML class diagrams from four industrial software projects, selected from a repository containing more than 2000 UML diagrams from 18 projects. Architectural maturity labels, assigned independently by an experienced software architect, serve as reference data for examining empirical thresholds for structural information. The results show that systematic weight calibration removes the undesirable behavior observed for the considered FBS16 configurations and provides a more stable basis for comparing UML class diagrams. The industrial analysis further indicates differences in the structural entropy ranges of commonly distinguished diagram categories. FBS26, therefore, provides a quantitative information-content measure that can support expert-based assessment of the architectural maturity of UML class diagrams. FAU - Daszczuk, Wiktor Bohdan AU - Daszczuk WB AUID- ORCID: 0000-0001-7532-362X AD - Institute of Computer Science, Warsaw University of Technology, Nowowiejska Str. 15/19, 00-665 Warsaw, Poland. FAU - Niepostyn, Stanislaw Jerzy AU - Niepostyn SJ AD - School of Computer Science & Technologies, VIZJA University, Okopowa Str. 59, 01-043 Warsaw, Poland. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13703011 OTO - NOTNLM OT - UML class diagrams OT - information content OT - normalized entropy OT - software architecture metrics OT - software measurement COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:37 PHST- 2026/08/19 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - e28101126 [pii] AID - entropy-28-01126 [pii] AID - 10.3390/e28101126 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 14;28(10):1126. doi: 10.3390/e28101126. PMID- 42940843 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 14 TI - A Novel Gear Fault Diagnosis Method Based on COA-VMD, RCMFE and SSA-BP. LID - 10.3390/e28101121 [doi] LID - 1121 AB - Aiming at the difficulties in extracting gear fault feature information and the unsatisfactory performance of commonly used machine learning classification models in terms of feature separability and stable within-distribution recognition, this paper proposes a fault diagnosis method combining variational mode decomposition (VMD) optimized by the coati optimization algorithm (COA) and a BP neural network optimized by the sparrow search algorithm (SSA). Firstly, a composite index based on permutation entropy and mutual information entropy is adopted as the fitness function, and the COA is used to optimize the key parameters of VMD to improve the decomposition accuracy of the VMD algorithm. Secondly, the intrinsic mode function (IMF) components sensitive to fault information are selected for signal reconstruction according to the time-frequency-domain comprehensive evaluation factor algorithm, and the refined composite multiscale fuzzy entropy (RCMFE) is used to extract the features of the reconstructed signal to form a state feature set. Finally, the SSA is employed to optimize the parameters of the BP model to achieve accurate diagnosis of gear working conditions and fault types. The proposed method is verified using measured gear single and compound fault data and compared with commonly used signal decomposition methods and machine learning classification models. The results show that the proposed method can effectively extract gear fault feature information and has the advantages of high accuracy, good feature separability and stable within-distribution recognition. It can achieve ideal diagnosis accuracy for both single and compound gear faults, providing a certain reference for solving such problems. FAU - Chen, Duowen AU - Chen D AD - Mechanical Engineering College, Beihua University, Jilin 132021, China. FAU - Lv, Haoze AU - Lv H AD - Mechanical Engineering College, Beihua University, Jilin 132021, China. FAU - Li, Xinze AU - Li X AD - Mechanical Engineering College, Beihua University, Jilin 132021, China. FAU - Liang, Wenjie AU - Liang W AD - Mechanical Engineering College, Beihua University, Jilin 132021, China. FAU - Bai, Kailin AU - Bai K AD - Mechanical Engineering College, Beihua University, Jilin 132021, China. FAU - Zhao, Xin AU - Zhao X AD - Mechanical Engineering College, Beihua University, Jilin 132021, China. FAU - Cao, Linlin AU - Cao L AUID- ORCID: 0000-0002-4894-6319 AD - Mechanical Engineering College, Beihua University, Jilin 132021, China. FAU - Zhou, Xiaolong AU - Zhou X AUID- ORCID: 0000-0002-0934-6046 AD - Mechanical Engineering College, Beihua University, Jilin 132021, China. LA - eng GR - 202610202019/the University-level College Students' Innovation and Entrepreneurship Training Program of Beihua University/ GR - YDZJ202601ZYTS224/Natural Science Foundation of Jilin Province (Free Exploration Project)/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13702893 OTO - NOTNLM OT - BP neural network OT - coati optimization algorithm OT - fault diagnosis OT - refined composite multiscale fuzzy entropy OT - sparrow search algorithm OT - variational mode decomposition COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:37 PHST- 2026/08/15 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - e28101121 [pii] AID - entropy-28-01121 [pii] AID - 10.3390/e28101121 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 14;28(10):1121. doi: 10.3390/e28101121. PMID- 42940840 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 13 TI - OneBoot GRU: Validation-Locked Polynomial Calibration for Level-Invariant Encrypted Inference. LID - 10.3390/e28101118 [doi] LID - 1118 AB - Encrypted recurrent inference must preserve both ciphertext depth and the numerical behavior of a feedback model. We develop OneBoot GRU, which couples the validation-locked selection of degree-three Sigmoid and degree-seven Tanh domains with a CKKS GRU cell that refreshes one hidden-state ciphertext after each step. On TwoPatterns, the locked polynomial model completed finite official-test trajectories with 92.431% macro accuracy, versus 98.114% for exact activations, and 0.830% mean domain violations. On SyntheticControl, polynomial and exact macro accuracies were 89.067% and 90.133%. Direct state-error comparisons show that improved domain coverage does not always reduce recurrent error; in a separate exploratory domain extension, screening reduced qualification tail error by 74.1% and 94.6% on ECG200 and ItalyPowerDemand. A parameter profile audited against pinned 128-bit sparse-secret references completed four encrypted recurrent steps, prefix pooling, and the trained head with maximum logit error 2.91 x 10(-6) relative to the plaintext polynomial circuit. In three matched four-step runs, GRU median circuit latency was 226.343 s versus 336.981 s for an implemented polynomial LSTM. Twelve additional distinct 128-step inputs were completed under an intentionally insecure structural profile, with 12/12 prediction agreement against the plaintext polynomial reference. These experiments provide measured calibration, arithmetic-fidelity, and system-cost operating points within their stated parameter and sampling scopes. FAU - Song, Xinxia AU - Song X AD - School of Big Data and Software Engineering, Zhejiang Wanli University, Ningbo 315100, China. FAU - Chen, Zhigang AU - Chen Z AD - College of Artificial Intelligence, Ningbo University of Finance and Economics, Ningbo 315175, China. FAU - Chen, Liqun AU - Chen L AD - Department of Computer Science, University of Surrey, Guildford GU2 7XH, UK. LA - eng GR - LMS26F020025/Zhejiang Provincial Natural Science Foundation/ GR - 2025J185/Ningbo Natural Science Foundation of China/ GR - 1320263702/Ningbo University of Finance & Economics/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13703041 OTO - NOTNLM OT - Bootstrapping OT - CKKS OT - gated recurrent unit OT - homomorphic encryption OT - polynomial domain calibration OT - trajectory fidelity COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:37 PHST- 2026/09/06 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - e28101118 [pii] AID - entropy-28-01118 [pii] AID - 10.3390/e28101118 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 13;28(10):1118. doi: 10.3390/e28101118. PMID- 42940836 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 12 TI - A Nonlinear Three-Mode Model of Solar Activity Based on Coupled Oscillators and Nonlinear Energy Transfer. LID - 10.3390/e28101114 [doi] LID - 1114 AB - Solar activity exhibits a dominant decadal timescale embedded in nonlinear multiscale dynamics. We present a minimal autonomous three-mode hybrid model to investigate whether such a timescale can arise from internal nonlinear energy exchange rather than external periodic forcing or direct inheritance of a prescribed modal period. The model couples a slow internal reservoir, a tachocline-associated transfer mode, and a nonlinear magnetic subsystem described by Duffing and Van der Pol-Duffing dynamics. Magnetic-energy release is implemented through a threshold-triggered reset, while a D-index-based structural energy-exchange index characterizes relative magnetic-energy changes. The system develops a persistent bounded hybrid regime with a dominant period of approximately 10 yr. Initial-condition ensembles and variations in the intrinsic modal periods demonstrate that this timescale does not simply track the prescribed 11 yr tachocline period. Parameter studies identify the release depth and magnetic recovery rate as stronger controls, supporting an accumulation-release mechanism of timescale selection. Release events exhibit organized recurrence and significant tachocline-phase preference, with localized event skipping separating spectral and release timescales. Saltation-based Lyapunov analysis reveals no robust positive largest exponent for the baseline regime over the investigated finite-time horizons. These results demonstrate that nonlinear energy exchange combined with threshold-mediated accumulation and release can autonomously generate a robust decadal collective timescale in a reduced nonlinear system. FAU - Kapytin, Vitaliy AU - Kapytin V AUID- ORCID: 0000-0002-2001-9847 AD - Ionosphere Institute, Almaty 050020, Kazakhstan. FAU - Andreyev, Alexey AU - Andreyev A AD - Ionosphere Institute, Almaty 050020, Kazakhstan. FAU - Zhumabayev, Beibit AU - Zhumabayev B AUID- ORCID: 0000-0002-8402-7934 AD - Ionosphere Institute, Almaty 050020, Kazakhstan. FAU - Yanina, Anastasiya AU - Yanina A AD - Institute of Plant Biology and Biotechnology, Almaty 050040, Kazakhstan. FAU - Chsherbulova, Yekaterina AU - Chsherbulova Y AUID- ORCID: 0009-0004-2518-8027 AD - Ionosphere Institute, Almaty 050020, Kazakhstan. FAU - Utebayev, Stanislav AU - Utebayev S AD - Ionosphere Institute, Almaty 050020, Kazakhstan. LA - eng GR - BR34837416/Aerospace committee of the Ministry of Artificial Intelligence and Digital Development of the Republic of Kazakhstan/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13703418 OTO - NOTNLM OT - Duffing oscillator OT - Van der Pol oscillator OT - d-index OT - magnetic energy release OT - nonlinear dynamics OT - reduced-order model OT - self-organization OT - solar activity OT - solar cycle OT - structural energy exchange COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:37 PHST- 2026/07/28 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - e28101114 [pii] AID - entropy-28-01114 [pii] AID - 10.3390/e28101114 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 12;28(10):1114. doi: 10.3390/e28101114. PMID- 42940834 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 12 TI - Complex Dynamics and Structure-Preserving Characteristics of a Three-Dimensional Discrete Cosine Memristive Map. LID - 10.3390/e28101112 [doi] LID - 1112 AB - A three-dimensional discrete map with state-dependent memristive feedback is constructed to examine complex dynamics persistence under finite parameter variations and perturbations. Fixed-point analysis, bifurcation diagrams, Lyapunov exponents (LEs), the 0-1 test, spectral entropy, and C0 complexity characterize the dynamics. At a=0.8, b=0.6, and c=0.4, positive finite-time maximum Lyapunov exponents support chaotic behavior while simulated trajectories remain finite. The original 1000-point scan yields 233 samples with lambdamax>10-3. A finer scan with step 0.0002 resolves four non-positive-LE samples within the previously reported positive-exponent region, and long-run validation retains their signs; thus, the finite grid does not establish a continuous chaotic interval. Feedback ablation distinguishes the combined model from isolated cosine and exponential components under the tested conditions. Initial-state diagnostics separate periodic initial-state equivalence and central inversion symmetry from translated attractor self-reproduction. Under selected parameter perturbations and Gaussian noise up to sigma=10-3, the principal trajectory structure remains identifiable. Comparisons with the logistic and Henon maps provide context. In National Institute of Standards and Technology (NIST) SP 800-22 testing, 146 of 148 non-overlapping template matching subtests meet the nominal 96/100 threshold, while two yield 95/100. These findings do not prove global boundedness, strict robust chaos, or cryptographic security. FAU - Liu, Zhaokun AU - Liu Z AD - School of Electronic Information, Xijing University, Xi'an 710123, China. FAU - Duan, Jinying AU - Duan J AD - School of Electronic Information, Xijing University, Xi'an 710123, China. LA - eng PT - Journal Article DEP - 20261012 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13703230 OTO - NOTNLM OT - Lyapunov exponent OT - complexity OT - discrete memristor OT - parameter perturbation OT - structure-preserving characteristics OT - three-dimensional chaotic map COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:37 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - e28101112 [pii] AID - entropy-28-01112 [pii] AID - 10.3390/e28101112 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 12;28(10):1112. doi: 10.3390/e28101112. PMID- 42940829 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 11 TI - Stochastic Dynamics of Sensitive and Resistant Bacteria Under Capacity-Limited Antibiotic Treatment. LID - 10.3390/e28101107 [doi] LID - 1107 AB - Antimicrobial resistance is a major challenge in the effective treatment of bacterial infections because antibiotic exposure can selectively suppress sensitive bacteria while allowing resistant populations to persist and expand. In this study, we develop a finite-state stochastic population model for the joint dynamics of antibiotic-sensitive and antibiotic-resistant bacteria under capacity-limited treatment. The model is formulated as a bivariate continuous-time Markov chain with density-dependent logistic growth, sensitive-to-resistant mutation, differential antibiotic susceptibility, finite carrying capacity, and bounded treatment-removal capacity. Because the extinction state is absorbing, the ordinary stationary distribution of the full finite-state process is concentrated at extinction, and long-lived non-extinct behavior is therefore analyzed through transient and quasi-stationary perspectives. Exact first-moment equations are derived, showing that mutation transfers individuals from the sensitive population to the resistant population without creating additional bacterial individuals. Local stability near extinction is characterized by the growth rates rs=lambdas-mu-nu and rr=lambdar-ϵmu. The positive equilibria of the deterministic fluid approximation are then derived under the different treatment-capacity regimes, while capacity-induced critical transitions are characterized through the equilibrium equations and the singularity condition detJ=0, where J is the Jacobian of the deterministic drift. Near a nondegenerate fold, the model exhibits square-root equilibrium scaling and critical slowing down. Gillespie Monte Carlo simulations complement the analytical results by quantifying finite-horizon survival probability, extinction time, conditional bacterial burden, resistant fraction, and cumulative bacterial exposure. The resulting framework provides a mathematically consistent description of how bacterial growth, mutation, differential susceptibility, and limited treatment capacity jointly influence extinction and long-lived persistence. FAU - Almutairi, A AU - Almutairi A AD - Department of Mathematics, College of Science, Qassim University, Buraydah 51452, Saudi Arabia. FAU - Sohaly, M A AU - Sohaly MA AUID- ORCID: 0000-0001-5971-0048 AD - Department of Statistics and Operations Research, College of Science, Qassim University, Buraydah 51452, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261011 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13702962 OTO - NOTNLM OT - Markov population process OT - antimicrobial resistance OT - bifurcation analysis OT - extinction OT - mathematical models simulation OT - mutation-selection dynamics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/11 CRDT- 2026/10/27 10:37 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - e28101107 [pii] AID - entropy-28-01107 [pii] AID - 10.3390/e28101107 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 11;28(10):1107. doi: 10.3390/e28101107. PMID- 42940824 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 10 TI - Robust Augmented-Diffusion Filtered-x Maximum Correntropy Criterion for Distributed Active Noise Control Under Impulsive Noise. LID - 10.3390/e28101102 [doi] LID - 1102 AB - Distributed multichannel active noise control (ANC) is scalable, but filtered-x least-mean-square updates are highly sensitive to impulsive disturbances. This paper proposes a robust augmented-diffusion filtered-x maximum correntropy criterion algorithm (R-ADFxMCC). It keeps the fixed admissible combination of augmented diffusion and replaces the local squared-error update with a normalized correntropy-gated recursion, whose kernel width follows a median-absolute-deviation estimate of the local residual scale. The resulting influence function is bounded and redescending. Under the stated independence and linearization approximations, the analysis gives a combination-weight-independent mean stability condition and a local steady-state excess mean-square error approximation; a symmetry argument proves zero steady-state mean bias without requiring independence between the gate and the regressors. In 30-realization simulations with one validation protocol for all step sizes, the median steady-state gains over augmented-diffusion FxLMS are 25.79, 21.84, 13.80 and 6.46 dB for single-tone, multitone, narrowband and broadband references under localized Bernoulli-Gaussian impulses. The augmented-diffusion M-estimate algorithm reaches the same steady state within 0.45 dB at the validation-selected step sizes, but on single-tone and narrowband references, R-ADFxMCC remains stable at step sizes at which the M-estimate algorithm diverges. Tests with alpha-stable noise, reverberation, secondary-path errors and measured per-sample cost are also reported. FAU - Peng, Dongming AU - Peng D AD - School of Electrical Engineering and Automation, Hefei University of Technology, Hefei 230009, China. FAU - He, Yigang AU - He Y AUID- ORCID: 0000-0002-6642-0740 AD - School of Electrical Engineering and Automation, Hefei University of Technology, Hefei 230009, China. AD - School of Electrical Engineering, Wuhan University, Wuhan 430072, China. FAU - Liu, Qianqian AU - Liu Q AD - School of Electrical Information Engineering, Jiangsu University of Technology, Changzhou 213001, China. LA - eng GR - 2024ZD0801300/the Smart Grid-National Science and Technology/ GR - 62601338/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13703330 OTO - NOTNLM OT - augmented diffusion OT - distributed active noise control OT - impulsive noise OT - maximum correntropy criterion OT - robust adaptive filtering COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:37 PHST- 2026/08/30 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - e28101102 [pii] AID - entropy-28-01102 [pii] AID - 10.3390/e28101102 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 10;28(10):1102. doi: 10.3390/e28101102. PMID- 42940820 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 9 TI - An Improved Classification Method Using a New Generalized Base Belief Function. LID - 10.3390/e28101098 [doi] LID - 1098 AB - Open-world classification must account for samples whose classes are absent from the current frame of discernment. Although generalized evidence theory can represent such possibilities, the conventional base belief function assigns reference support only to nonempty propositions and therefore cannot directly support source-level treatment of unknown information. This work proposes a generalized base belief function that incorporates the open-world proposition into its reference domain and uses it to modify generalized basic probability assignments before fusion. The modified evidence is combined through rules compatible with generalized evidence theory and followed by a known/unknown decision, forming a complete classification procedure for incomplete frames. Experiments on Iris and Seeds show that source-level modification and generalized fusion jointly support known/unknown classification. On Waveform, classification remains stable as controlled perturbation increases in a larger, higher-dimensional, noisy environment. On the Wall-Following Robot dataset, the procedure gives a selective known/unknown decision across different class compositions, preserving known samples while retaining overall discrimination close to GCR. These results support the proposed GBBF-based method for open-world classification under incomplete frames. FAU - Wei, Qi AU - Wei Q AUID- ORCID: 0009-0008-1094-8176 AD - School of Integrated Circuits and Microelectronics, Northwestern Polytechnical University, Xi'an 710072, China. AD - Shenzhen Research Institute of Northwestern Polytechnical University, Shenzhen 518057, China. FAU - Qiang, Shiqi AU - Qiang S AD - School of Integrated Circuits and Microelectronics, Northwestern Polytechnical University, Xi'an 710072, China. AD - Shenzhen Research Institute of Northwestern Polytechnical University, Shenzhen 518057, China. FAU - Li, Hangjun AU - Li H AUID- ORCID: 0009-0005-0788-283X AD - School of Automation, Northwestern Polytechnical University, Xi'an 710072, China. FAU - He, Shan AU - He S AD - School of Integrated Circuits and Microelectronics, Northwestern Polytechnical University, Xi'an 710072, China. AD - Shenzhen Research Institute of Northwestern Polytechnical University, Shenzhen 518057, China. FAU - Liu, Junqiang AU - Liu J AUID- ORCID: 0009-0005-0257-4272 AD - School of Integrated Circuits and Microelectronics, Northwestern Polytechnical University, Xi'an 710072, China. AD - Shenzhen Research Institute of Northwestern Polytechnical University, Shenzhen 518057, China. FAU - Hou, Longxiang AU - Hou L AD - School of Integrated Circuits and Microelectronics, Northwestern Polytechnical University, Xi'an 710072, China. AD - Shenzhen Research Institute of Northwestern Polytechnical University, Shenzhen 518057, China. FAU - Tang, Yongchuan AU - Tang Y AUID- ORCID: 0000-0003-2568-9628 AD - School of Integrated Circuits and Microelectronics, Northwestern Polytechnical University, Xi'an 710072, China. AD - Shenzhen Research Institute of Northwestern Polytechnical University, Shenzhen 518057, China. LA - eng GR - 62301439/National Natural Science Foundation of China/ GR - 2025A1515012205/Guangdong Basic and Applied Basic Research Foundation/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13703660 OTO - NOTNLM OT - Dempster-Shafer evidence theory OT - conflict data fusion OT - evidence modification OT - generalized base belief function OT - open-world classification COIS- The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:37 PHST- 2026/09/07 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - e28101098 [pii] AID - entropy-28-01098 [pii] AID - 10.3390/e28101098 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 9;28(10):1098. doi: 10.3390/e28101098. PMID- 42940818 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 8 TI - Forecasting Explosive Bubble Regimes in Bitcoin Time Series with Multi-Step Ensemble Learning. LID - 10.3390/e28101096 [doi] LID - 1096 AB - Bitcoin bubbles exhibit locally explosive dynamics, but identifying past episodes does not establish their predictability. We combine the generalized supremum augmented Dickey-Fuller test with nine probabilistic learners and three probability-output ensembles to forecast bubble states over one-, three-, and seven-day horizons. Using daily data from January 2016 to April 2026, we identify 30 maximal uninterrupted episodes comprising 455 bubble days. Development-period validation selects the lookback window and oversampling ratios before final-test evaluation; ensemble weights are estimated from development-period rolling forecasts. No model family dominates across horizons. The constrained least-squares ensemble has the lowest final-test mean squared error at one day, whereas logistic regression has the lowest error at three and seven days. The inverse-error-weighted ensemble has the highest average precision at seven days. Moving-block bootstrap intervals overlap substantially, and the 90% Model Confidence Set retains several models at every horizon. A cross-source robustness check yields 99.58% agreement in daily bubble-state classifications. These findings support horizon-dependent probabilistic risk monitoring, while the limited number of independent episodes and low recall at longer horizons constrain the practical interpretation of the forecasts. FAU - Li, Jian-Rong AU - Li JR AD - School of Finance, Yunnan University of Finance and Economics, Kunming 650221, China. FAU - Zhong, Guang-Yan AU - Zhong GY AD - School of Finance, Yunnan University of Finance and Economics, Kunming 650221, China. FAU - Li, Jiang-Cheng AU - Li JC AD - School of Finance, Yunnan University of Finance and Economics, Kunming 650221, China. LA - eng GR - 72663029/National Natural Science Foundation of China/ GR - 72163035/National Natural Science Foundation of China/ GR - 72563033/National Natural Science Foundation of China/ GR - 23YJCZH322/Ministry of Education of Humanities and Social Science Project of China/ GR - 202501AS070167/Applied Basic Research Foundation of Yunnan Province/ GR - 202401AT070276/Applied Basic Research Foundation of Yunnan Province/ GR - 2026Y0827/Yunnan Provincial Department of Education Science Research Fund Project/ PT - Journal Article DEP - 20261008 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13703411 OTO - NOTNLM OT - Bitcoin bubble OT - Model Confidence Set OT - ensemble learning OT - explosive regimes OT - multi-step forecasting OT - time series COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:37 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - e28101096 [pii] AID - entropy-28-01096 [pii] AID - 10.3390/e28101096 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 8;28(10):1096. doi: 10.3390/e28101096. PMID- 42940815 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 8 TI - Measurement Entropy and Conditional Information in Time-Reversed Young Interferometry. LID - 10.3390/e28101093 [doi] LID - 1093 AB - Time-reversed Young (TRY) interferometry holds a detector channel fixed while a source coordinate is scanned or programmed. We describe this measurement through a single accepted source-detector probability law obtained from scalar Fresnel propagation, the programmed source prior, and detector acceptance. The conventional fixed-source Young distribution, the source and detector marginals, and the fixed-detector TRY distribution are then different reductions of that common record. Standard information measures can therefore be assigned without ambiguity to the probability law actually measured. For a symmetric double-slit response with matched source and detector windows, the fixed-source and fixed-detector conditional distributions have the same entropy; the physically useful information from detector conditioning is instead expressed by grid-stable entropy deficits and Kullback-Leibler information gains. The canonical example gives a modest prior-to-posterior information gain and establishes a transparent baseline for more selective source programming, detector modes, or operating points. Finite slit width, finite-source element width, finite detector pixels, and finite-count entropy estimation are included to connect the ideal model with experiments. A phase scan then shows that conditional entropy and Fisher information characterize different properties of the same likelihood, while a two-mode coherence model separates record entropy from optical coherence entropy and distinguishes coherent superposition from incoherent mixture. The framework gives a unified statistical interpretation of fixed-detector, source-programmed Young interferometry while keeping optical reciprocity, Bayesian conditioning, local parameter sensitivity, and quantum state disturbance conceptually distinct. FAU - Wen, Jianming AU - Wen J AUID- ORCID: 0000-0003-3373-7108 AD - Department of Electrical and Computer Engineering, Binghamton University, Binghamton, NY 13902, USA. LA - eng GR - Binghamton University/ GR - 105383/Watson College/ PT - Journal Article DEP - 20261008 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13703189 OTO - NOTNLM OT - Kullback-Leibler divergence OT - Shannon entropy OT - conditional information OT - mutual information OT - optical coherence OT - optical reciprocity OT - statistical optics OT - time-reversed Young interferometry COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:37 PHST- 2026/09/05 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - e28101093 [pii] AID - entropy-28-01093 [pii] AID - 10.3390/e28101093 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 8;28(10):1093. doi: 10.3390/e28101093. PMID- 42940814 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 7 TI - Generalized Weighted Ordinal Pattern Descriptors for the Characterization of Adventitious Respiratory Sounds. LID - 10.3390/e28101092 [doi] LID - 1092 AB - Automatic characterization of adventitious respiratory sounds is essential for developing computer-aided systems that support respiratory disease diagnosis. Commonly used acoustic descriptors, such as Mel frequency cepstral coefficients (MFCCs), primarily characterize spectral information and may not fully capture the nonlinear temporal dynamics of respiratory signals. In this work, we investigate the discriminative capability of Generalized Weighted Ordinal Pattern (GWOP) descriptors for representing adventitious respiratory sounds. Using respiratory recordings from the publicly available SPRSound pediatric database, GWOP descriptors were computed over multiple embedding dimensions, weighting factors, and time delays. An XGBoost classifier was employed exclusively as a supervised validation model to assess the discriminative information contained in the proposed feature representation, while the XGBoost Gain criterion was used to analyze the contribution of individual descriptor components. The proposed representation achieved stable performance across cross-validation folds, outperformed a conventional MFCC representation under the same evaluation protocol, and demonstrated that a reduced subset of Gain selected features preserved, and slightly improved, the classification performance while substantially reducing the dimensionality of the original feature space. These findings indicate that GWOP provides a lighter yet more informative and interpretable representation of adventitious respiratory sounds, capturing nonlinear ordinal dynamics that complement conventional spectral descriptors and offering a promising alternative for respiratory sound characterization. FAU - Lopez Perez, Jorge I AU - Lopez Perez JI AUID- ORCID: 0000-0001-7132-676X AD - Departamento de Ingenieria Electrica y Computadoras, Instituto de Ciencias e Ingenieria de la Computacion, Universidad Nacional del Sur-Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Bahia Blanca 8000, Argentina. FAU - Duarte, Cristina D AU - Duarte CD AUID- ORCID: 0009-0007-8034-4987 AD - Departamento de Fisica, Instituto de Fisica del Sur, Universidad Nacional del Sur-Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Bahia Blanca 8000, Argentina. FAU - Delrieux, Claudio A AU - Delrieux CA AUID- ORCID: 0000-0002-2727-8374 AD - Departamento de Ingenieria Electrica y Computadoras, Instituto de Ciencias e Ingenieria de la Computacion, Universidad Nacional del Sur-Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Bahia Blanca 8000, Argentina. LA - eng GR - PGI 24/K093/Universidad Nacional del Sur/ PT - Journal Article DEP - 20261007 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13703545 OTO - NOTNLM OT - adventitious respiratory sounds OT - generalized weighted ordinal patterns OT - ordinal patterns OT - permutation entropy OT - respiratory sound analysis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/07 CRDT- 2026/10/27 10:37 PHST- 2026/08/27 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - e28101092 [pii] AID - entropy-28-01092 [pii] AID - 10.3390/e28101092 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 7;28(10):1092. doi: 10.3390/e28101092. PMID- 42940806 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Oct 1 TI - Bridging Machine Learning and Algorithmic Information Theory, Part VII: Algorithmic Information Kernels and Kernel Discrepancies on Countable Spaces. LID - 10.3390/e28101084 [doi] LID - 1084 AB - Compression-based dissimilarities such as the normalized compression distance are widely used, but direct exponentiation need not produce a positive semidefinite kernel. We develop a systematic interface between prefix algorithmic information theory and three kernel discrepancy methods on finite or countable spaces: maximum mean discrepancy (MMD), the Hilbert-Schmidt independence criterion (HSIC), and a Markov-operator kernel Stein discrepancy (KSD). Explicit feature realizations guarantee positive semidefiniteness for distance-to-kernel embeddings and for an ideal Solomonoff feature-mixture kernel satisfying kSol,U(x,y) asymptotically equal toU2-KU(⟨x,y⟩). Zero-mass signed measures give the exact characteristicity criterion, and whether the ideal Solomonoff kernel meets that criterion turns out to depend on the universal machine: two universal prefix machines are exhibited, one whose kernel is not characteristic on a two-point set and one whose kernel separates all finite-total-variation signed measures on the countable string space. In the resulting RKHS geometry, the MMD compares compressibility profiles, the HSIC is product-kernel MMD from the joint law to the product of its marginals, and KSDTP,k(Q)=MMDk(QTP,Q) for a target-stationary Markov kernel TP. Expected algorithmic mutual information is connected to the HSIC only through explicit source-description, mutual-information, support, and kernel conditions. Ideal AIT objects, computable feature surrogates, and finite-sample errors are separated explicitly. Reproducible synthetic experiments examine the identities, landmark approximation, and selected testing alternatives at the computable and empirical layers. They provide no evidence about ideal AIT quantities or general superiority over baselines. An algebraic example also shows that a characteristic finite-support kernel can give a constant permutation statistic: identification alone does not guarantee test power. FAU - Hamzi, Boumediene AU - Hamzi B AD - Department of Computing and Mathematical Sciences, California Institute of Technology, Pasadena, CA 91125, USA. AD - The Alan Turing Institute, London NW1 2DB, UK. FAU - Hutter, Marcus AU - Hutter M AUID- ORCID: 0000-0002-3263-4097 AD - Google DeepMind, London N1C 4DJ, UK. AD - School of Computing, Australian National University, Canberra, ACT 2601, Australia. FAU - Owhadi, Houman AU - Owhadi H AUID- ORCID: 0000-0002-5677-1600 AD - Department of Computing and Mathematical Sciences, California Institute of Technology, Pasadena, CA 91125, USA. LA - eng PT - Journal Article DEP - 20261001 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13702998 OTO - NOTNLM OT - Hilbert-Schmidt independence criterion OT - Kolmogorov complexity OT - Solomonoff induction OT - algorithmic mutual information OT - characteristic kernels OT - hypothesis testing OT - kernel Stein discrepancy OT - maximum mean discrepancy OT - normalized compression distance OT - reproducing kernel Hilbert space COIS- Author Marcus Hutter was employed by the company Google DeepMind. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:37 PHST- 2026/08/11 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - e28101084 [pii] AID - entropy-28-01084 [pii] AID - 10.3390/e28101084 [doi] PST - epublish SO - Entropy (Basel). 2026 Oct 1;28(10):1084. doi: 10.3390/e28101084. PMID- 42940805 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Sep 30 TI - The Distribution of Lexical Information Within Dependency Spans: Cross-Linguistic Evidence from 22 Languages. LID - 10.3390/e28101083 [doi] LID - 1083 AB - Dependency distance measures the linear separation between syntactically related words but does not describe how information is distributed across their interval. We analyzed summed XGLM-2.9B word surprisal in 1,346,122 dependency spans from 22 Universal Dependencies languages at distances 4-10. Clustering 154 position-aligned profiles yielded a broadly descending type and a type with a terminal rise; 20 languages retained their assignment across distances. Leave-one-language-out comparison did not support a common three-segment structure: the one-standard-error rule selected two segments for the descending type and three for the rising type. The descending type's initial decline disappeared after separate adjustments for sentence position and preceding context. Standardizing word-length and model subword-count distributions reduced the rising type's mean final-word increase from +1.996 to +0.408 bits (95% interval [-0.024, +0.898]). In held-out sentences, real dependencies in eight languages independently classified by a training-set terminal decrease by 0.928 bits more than nearby nonedge intervals sharing the final part-of-speech tag (95% interval [-1.498, -0.217]). A shared part-of-speech component was negatively associated with terminal surprisal in the original descending type. Dependency-span alignment reveals recurring information profiles and locates a conditional terminal contrast for further tests of syntactic prediction. FAU - Kang, Xiangyu AU - Kang X AD - School of Linguistics and Language Resources (Institute of Linguistic Sciences), Beijing Language and Culture University, 15 Xueyuan Road, Haidian District, Beijing 100083, China. FAU - Yu, Shuiyuan AU - Yu S AD - School of Linguistics and Language Resources (Institute of Linguistic Sciences), Beijing Language and Culture University, 15 Xueyuan Road, Haidian District, Beijing 100083, China. LA - eng PT - Journal Article DEP - 20260930 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13702879 OTO - NOTNLM OT - cross-linguistic comparison OT - dependency distance OT - dependency span OT - lexical composition OT - sentence position OT - word surprisal COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:37 PHST- 2026/08/18 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - e28101083 [pii] AID - entropy-28-01083 [pii] AID - 10.3390/e28101083 [doi] PST - epublish SO - Entropy (Basel). 2026 Sep 30;28(10):1083. doi: 10.3390/e28101083. PMID- 42940803 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Sep 30 TI - Finite-Bandwidth Protection of a Three-Level Quantum Heat Engine Against Parasitic Heat Leaks. LID - 10.3390/e28101081 [doi] LID - 1081 AB - Finite-bandwidth reservoir engineering can suppress unwanted transitions in a quantum thermal machine, but a physical filter also introduces a finite response time. We study this competition in a continuous three-level heat engine whose hot environment couples parasitically to the cold transition. The corresponding three-state rate network is solved exactly, showing that the parasitic transition produces a hot-to-cold thermodynamic short circuit and yielding a closed threshold for the loss of positive-power operation. We then retain a damped auxiliary mode explicitly as a physical spectral filter. Its Lorentzian response suppresses the detuned parasitic transition, whereas excessive narrowing limits the useful energy throughput. Independent local-GKSL and nonsecular Bloch-Redfield calculations both recover the no-leak Markovian engine in the weak-coupling limit and predict a finite maximum-power bandwidth, although its precise location is model dependent. A frequency-resolved Lorentzian-rate reduction, by contrast, has no interior optimum. The resulting design principle is that spectral selectivity can protect the useful thermodynamic cycle, but a real filter cannot be narrowed without a dynamical throughput cost. FAU - Prataviera, Gilberto Aparecido AU - Prataviera GA AUID- ORCID: 0000-0002-0628-2243 AD - Departamento de Administracao, Faculdade de Economia, Administracao e Contabilidade de Ribeirao Preto (FEA-RP), Universidade de Sao Paulo, Ribeirao Preto 14040-905, SP, Brazil. FAU - de Oliveira, Marcos Cesar AU - de Oliveira MC AUID- ORCID: 0000-0003-2251-2632 AD - Instituto de Fisica Gleb Wataghin, Universidade Estadual de Campinas, Campinas 13083-859, SP, Brazil. LA - eng GR - 2024/00998-6/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/ GR - 08884/2024-0/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ PT - Journal Article DEP - 20260930 PL - Switzerland TA - Entropy (Basel) JT - Entropy (Basel, Switzerland) JID - 101243874 PMC - PMC13703888 OTO - NOTNLM OT - Bloch-Redfield equation OT - GKSL master equation OT - finite-bandwidth reservoir OT - open quantum systems OT - parasitic heat leak OT - quantum heat engine OT - reservoir engineering COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:37 PHST- 2026/09/03 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - e28101081 [pii] AID - entropy-28-01081 [pii] AID - 10.3390/e28101081 [doi] PST - epublish SO - Entropy (Basel). 2026 Sep 30;28(10):1081. doi: 10.3390/e28101081. PMID- 42940795 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1099-4300 (Electronic) IS - 1099-4300 (Linking) VI - 28 IP - 10 DP - 2026 Sep 30 TI - Sample-Conditional Mixtures of Entropy Estimators for Short Sequences Under a Uniform Marginal Null. LID - 10.3390/e28101073 [doi] LID - 1073 AB - Entropy estimation from short samples recurs in symmetric cryptography, where the reference distribution is uniform by design and no single estimator in the considered classical comparison set minimizes mean squared error (MSE) across the full range of ratios n/k. We introduce the adaptive sample-conditional entropy diagnostic (ASED), in which a compact network trained offline maps a frequency-of-frequencies descriptor of the sample to convex mixture weights over six classical estimators, at a cost of O(n+k). We prove an oracle inequality bounding the excess risk of such a mixture by the L1 error of its weights and fixed-alphabet consistency for every simplex-valued weighting rule, independently of the distribution used to train the weights. Under the uniform-null protocol, ASED attains an integrated MSE of 1.7x10-3 for bytes, compared with 3.8x10-2 for James-Stein shrinkage, a reduction that depends materially on the aggregation scheme: 95.4% for summed or averaged MSE across sample sizes versus 24% for the mean of per-size ratios. Both figures are reported together throughout, and neither is presented as the headline; the entire advantage is confined to the undersampled regime n/=k. A locked train/validate/test evaluation with an independently written implementation reproduces the reduction at 95.1%. Because a constant output achieves zero error by construction here, we add further checks: in a post hoc analysis, ASED is non-inferior to SHR in detection power at a 0.02 margin, both pointwise and simultaneously, whereas a constant control has none, and the advantage persists on held-out configurations. The 0.02 margin was selected after inspecting the power estimates, and at n=8 and n=16, it is smaller than the variation induced by tie handling at the empirical critical value, so the non-inferiority conclusion is confirmatory only for n>/=32. At n/=45 weeks CGA). Clinical characteristics, treatment modalities, and adverse events were collected and compared between groups. Results: Seventy-five patients were included: 9 received early OP (mean CGA at treatment initiation 38 weeks + 3 days) and 66 that started treatment at or after 45 weeks CGA. Early-treated infants showed a higher prevalence of complicated IH at diagnosis (88.9% vs. 4.5%). No major adverse events, including clinically significant bradycardia, hypotension, arrhythmias, respiratory complications, or treatment discontinuation, occurred in either group. Transient asymptomatic hypoglycemia occurred in five patients, all belonging to the standard-timing group, and resolved with feeding. Treatment duration was comparable between groups. Conclusions: Early OP administration before 45 weeks CGA may represent a feasible and well-tolerated option in selected preterm infants and term newborn with complicated high-risk IH when initiated in a protected neonatal setting with careful monitoring. These findings may suggest individualized risk-based decision-making in selected cases, rather than routine treatment before current age thresholds. FAU - Pensabene, Marco AU - Pensabene M AUID- ORCID: 0000-0003-3187-1901 AD - Pediatric Surgery Unit, Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties "G. D'Alessandro", University of Palermo, 90133 Palermo, Italy. FAU - Patti, Maria AU - Patti M AD - Pediatric Surgery Unit, Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties "G. D'Alessandro", University of Palermo, 90133 Palermo, Italy. FAU - Serra, Gregorio AU - Serra G AUID- ORCID: 0000-0002-2918-9826 AD - Neonatal Intensive Care Unit, Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties "G. D'Alessandro", University of Palermo, 90133 Palermo, Italy. FAU - Baldanza, Fabio AU - Baldanza F AD - Pediatric Surgery Unit, Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties "G. D'Alessandro", University of Palermo, 90133 Palermo, Italy. FAU - Grasso, Francesco AU - Grasso F AUID- ORCID: 0009-0004-4520-7766 AD - Pediatric Surgery Unit, Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties "G. D'Alessandro", University of Palermo, 90133 Palermo, Italy. FAU - Giuffre, Mario AU - Giuffre M AUID- ORCID: 0000-0001-9576-1635 AD - Neonatal Intensive Care Unit, Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties "G. D'Alessandro", University of Palermo, 90133 Palermo, Italy. FAU - Licata Ricottone, Giovanni AU - Licata Ricottone G AD - Pediatric and New-born Unit, Bressanone Hospital, Azienda Sanitaria dell'Alto Adige (SABES), Via Dante 51, 39042 Bressanone, Italy. FAU - Sergio, Maria AU - Sergio M AUID- ORCID: 0000-0003-2433-6281 AD - Pediatric Surgery Unit, Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties "G. D'Alessandro", University of Palermo, 90133 Palermo, Italy. FAU - Corsello, Giovanni AU - Corsello G AD - Neonatal Intensive Care Unit, Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties "G. D'Alessandro", University of Palermo, 90133 Palermo, Italy. FAU - Di Pace, Maria Rita AU - Di Pace MR AUID- ORCID: 0000-0003-2170-8035 AD - Pediatric Surgery Unit, Department of Health Promotion, Mother and Child Care, Internal Medicine and Medical Specialties "G. D'Alessandro", University of Palermo, 90133 Palermo, Italy. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13702952 OTO - NOTNLM OT - beta-adrenergic blockers OT - infantile hemangioma OT - premature infants OT - propranolol OT - vascular tumors COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:37 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - children13101435 [pii] AID - children-13-01435 [pii] AID - 10.3390/children13101435 [doi] PST - epublish SO - Children (Basel). 2026 Oct 19;13(10):1435. doi: 10.3390/children13101435. PMID- 42940750 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Oct 18 TI - Congenital Hyperinsulinism in the Era of Precision Medicine: Genetically Driven Diagnosis and Novel Therapies. LID - 10.3390/children13101431 [doi] LID - 1431 AB - BACKGROUND/OBJECTIVES: Congenital hyperinsulinism (CHI) is the leading cause of persistent hypoglycaemia in infancy and childhood characterized by dysregulated insulin secretion despite low plasma glucose concentrations. Early recognition and treatment are essential to reduce the risk of neurological injury. Advances in molecular genetics have revealed substantial genetic and phenotypic heterogeneity beyond classical K(ATP)-channel disorders. This review examines the expanding genetic landscape of CHI and the translation of molecular discoveries into precision clinical care. METHODS: This narrative review, based on targeted literature searches of PubMed/MEDLINE, Scopus and Embase and expert selection of key publications, synthesizes current evidence on the genetic mechanisms, genotype-phenotype relationships, molecular diagnosis, and clinical management of CHI, with emphasis on established and emerging genetic subtypes and their implications for therapeutic decision-making. RESULTS: More than 30 disease-associated genes have been identified, involving ion channels, metabolic enzymes, transcription factors, membrane transport proteins, mitochondrial pathways, and developmental mechanisms. Major genetic forms involving ABCC8, KCNJ11, GLUD1, GCK, HK1, HADH, SLC16A1, HNF4A, HNF1A, and FOXA2 illustrate diverse mechanisms of dysregulated beta-cell insulin secretion. Recognition of non-coding regulatory variants has further expanded the molecular spectrum and provided new insights into tissue-specific gene regulation. Molecular diagnosis increasingly informs prediction of diazoxide responsiveness, selection for (18)F-DOPA PET/CT, identification of patients at high risk of focal disease, surgical planning, genetic counselling, and long-term surveillance. CONCLUSIONS: Precision medicine is increasingly transforming CHI from a predominantly phenotype-based disorder into a genetically informed clinical entity. Integration of genomic testing with clinical phenotyping and functional assessment can improve diagnostic classification and individualize management. Continued genomic discovery, functional validation, and international collaboration will be important for patients who remain without a molecular diagnosis and for developing mechanism-based therapies. FAU - Contractor, Jaikumar B AU - Contractor JB AUID- ORCID: 0000-0003-4915-4097 AD - Department of Anatomy, GMERS Medical College, Panchmahal, Godhra 389120, Gujarat, India. AD - Congenital Hyperinsulinism India Association, Anand 388325, Gujarat, India. FAU - Bharti, Lalankumar AU - Bharti L AD - Jag Pravesh Chandra Hospital, Government of Delhi, New Delhi 110053, India. FAU - Shah, Ishan AU - Shah I AD - Congenital Hyperinsulinism India Association, Anand 388325, Gujarat, India. FAU - Shah, Stuti AU - Shah S AUID- ORCID: 0009-0001-6512-8828 AD - Congenital Hyperinsulinism India Association, Anand 388325, Gujarat, India. AD - Parul Institute of Medical Sciences & Research, Vadodara 391760, Gujarat, India. FAU - Nimbalkar, Somashekhar AU - Nimbalkar S AD - Congenital Hyperinsulinism India Association, Anand 388325, Gujarat, India. AD - All India Institute of Medical Sciences, Deoghar 814117, Jharkhand, India. FAU - Bajpai, Anurag AU - Bajpai A AD - Regency Center for Diabetes, Endocrinology & Research (CDER), Kanpur 208001, Uttar Pradesh, India. FAU - Kumar, Ravindra AU - Kumar R AD - Hindu Rao Hospital & North Delhi Municipal Corporation Medical College, Delhi 110007, India. FAU - Mohan, Viswanathan AU - Mohan V AUID- ORCID: 0000-0001-5038-6210 AD - Madras Diabetes Research Centre, ICMR Collaborating Centre of Excellence, Chennai 600086, Tamil Nadu, India. AD - Dr. Mohan's Diabetes Specialities Centre, IDF Centre of Excellence in Diabetes Care, Chennai 600086, Tamil Nadu, India. FAU - Radha, Venkatesan AU - Radha V AD - Madras Diabetes Research Centre, ICMR Collaborating Centre of Excellence, Chennai 600086, Tamil Nadu, India. FAU - Shah, Pratik AU - Shah P AUID- ORCID: 0000-0002-4402-8297 AD - Congenital Hyperinsulinism India Association, Anand 388325, Gujarat, India. AD - The Royal London Childrens Hospital, Barts Health NHS Trust, E1 1FR & Queen Mary University of London, London EC1M 6BQ, UK. LA - eng PT - Journal Article PT - Review DEP - 20261018 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13702925 OTO - NOTNLM OT - 18F-DOPA PET/CT OT - KATP channel mutations OT - diazoxide-unresponsive hypoglyacemia OT - genotype-phenotype correlation OT - hyperinsulinism COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:37 PHST- 2026/08/23 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - children13101431 [pii] AID - children-13-01431 [pii] AID - 10.3390/children13101431 [doi] PST - epublish SO - Children (Basel). 2026 Oct 18;13(10):1431. doi: 10.3390/children13101431. PMID- 42940744 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Oct 17 TI - Functional Orthodontic Rehabilitation and Craniofacial Growth in Pediatric Mandibular Condylar Fractures: A Narrative Review. LID - 10.3390/children13101425 [doi] LID - 1425 AB - BACKGROUND/OBJECTIVES: Pediatric mandibular condylar fractures require management that accounts for ongoing craniofacial growth and temporomandibular joint (TMJ) function. This narrative review summarizes contemporary evidence on age- and displacement-sensitive decision-making, functional and orthodontic rehabilitation, imaging, and long-term growth surveillance. METHODS: A structured narrative search of PubMed/MEDLINE, Scopus, Web of Science, and the Cochrane Library was conducted for English-language human studies published from January 2021 through June 2026, supplemented by citation searching for foundational publications. RESULTS: Management ranges from expectant observation and functional appliance therapy to brief closed stabilization and open reduction with internal fixation (ORIF). Predominantly retrospective and heterogeneous evidence suggests that timely restoration of comfortable mandibular mobility is associated with favorable functional recovery and substantial remodeling potential in many growing patients, although outcomes depend on age, fracture morphology, functional loading, and remaining growth. ORIF may be appropriate for selected severe fractures when acceptable function and occlusion cannot be restored by less invasive measures. CONCLUSIONS: Management should integrate skeletal maturity, fracture level and displacement, laterality, occlusal disturbance, mandibular mobility, cooperation, and remaining growth. Across treatment modalities, avoidance of unnecessary prolonged immobilization, structured functional rehabilitation, and longitudinal orthodontic and craniofacial surveillance are important clinical priorities. FAU - Giannini, Lucia AU - Giannini L AUID- ORCID: 0000-0002-3132-1750 AD - Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy. FAU - Gaffuri, Francesca AU - Gaffuri F AUID- ORCID: 0000-0002-2132-4093 AD - Department of Biomedical, Surgical and Dental Sciences, University of Milan, 20122 Milan, Italy. FAU - Cattaneo, Giovanni AU - Cattaneo G AUID- ORCID: 0009-0005-9624-7036 AD - Department of Biomedical, Surgical and Dental Sciences, University of Milan, 20122 Milan, Italy. FAU - Maspero, Cinzia AU - Maspero C AUID- ORCID: 0000-0002-5930-1698 AD - Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy. AD - Department of Biomedical, Surgical and Dental Sciences, University of Milan, 20122 Milan, Italy. LA - eng GR - XXXX/Italian Ministry of Health/ PT - Journal Article PT - Review DEP - 20261017 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703360 OTO - NOTNLM OT - adolescent OT - bone remodeling OT - child OT - fracture fixation, internal OT - mandibular condyle/injuries OT - mandibular fractures OT - orthodontic appliances, functional OT - temporomandibular joint COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:37 PHST- 2026/09/20 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - children13101425 [pii] AID - children-13-01425 [pii] AID - 10.3390/children13101425 [doi] PST - epublish SO - Children (Basel). 2026 Oct 17;13(10):1425. doi: 10.3390/children13101425. PMID- 42940736 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Oct 15 TI - Congenital Diaphragmatic Hernia Requiring ECMO: Current Evidence, Controversies, and Unresolved Questions. LID - 10.3390/children13101417 [doi] LID - 1417 AB - Background/Objectives: Congenital diaphragmatic hernia (CDH) requiring extracorporeal membrane oxygenation (ECMO) remains one of the most challenging conditions in neonatal critical care. Despite advances in prenatal assessment and neonatal management, substantial uncertainty persists regarding ECMO selection, mode and duration of support, and timing of diaphragmatic repair. This review summarizes the available evidence and critically examines the major unresolved questions in the management of infants with CDH requiring ECMO. Methods: A narrative review was conducted based on a literature search in PubMed/MEDLINE, Scopus, Web of Science, and the Cochrane Library for studies published between June 2005 and June 2026. Additional relevant publications were identified through reference screening, and selected foundational studies predating the search period were included when appropriate. Prospective and retrospective studies, registry-based analyses, systematic reviews, meta-analyses, and relevant consensus statements were considered. Evidence was narratively synthesized, with particular attention to disease severity, methodological limitations, and survivor bias. Results: Available evidence highlights substantial heterogeneity in disease severity and management. Prenatal and postnatal markers aid risk stratification, but no single parameter reliably predicts ECMO requirement or defines the optimal management strategy. To date, no clear survival advantage has been demonstrated for either veno-arterial or veno-venous ECMO, although veno-arterial ECMO remains the predominant mode of support in most centers; mode selection should therefore be individualized according to cardiopulmonary physiology, patient characteristics, anatomy, and institutional expertise. Surgical timing remains particularly controversial. While repair after ECMO decannulation has been associated with favorable outcomes, recent observational analyses suggest that early repair during ECMO may be beneficial in selected patients, although the optimal timing remains uncertain. Prolonged ECMO may provide additional time for pulmonary and cardiovascular recovery, although this potential benefit must be weighed against the cumulative morbidity of prolonged support. Conclusions: Management should be individualized according to disease severity, clinical trajectory, cardiopulmonary recovery, and institutional expertise. Current evidence does not support a universal strategy for ECMO initiation, mode, surgical timing, or duration. Prospective multicenter studies using standardized definitions and adequately accounting for disease severity are needed to reduce practice variation and better define optimal management. FAU - Pertierra Cortada, Africa AU - Pertierra Cortada A AUID- ORCID: 0000-0001-8596-7840 AD - Neonatology Department, BCNatal-Centre de Medicina Maternofetal i Neonatologia de Barcelona, Hospital Sant Joan de Deu, Universitat de Barcelona, 08950 Barcelona, Spain. AD - Institut de Recerca Sant Joan de Deu, 08950 Barcelona, Spain. FAU - Carrasco Carrasco, Cristina AU - Carrasco Carrasco C AD - Neonatology Department, BCNatal-Centre de Medicina Maternofetal i Neonatologia de Barcelona, Hospital Sant Joan de Deu, Universitat de Barcelona, 08950 Barcelona, Spain. AD - Institut de Recerca Sant Joan de Deu, 08950 Barcelona, Spain. LA - eng PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703401 OTO - NOTNLM OT - ECMO candidacy OT - ECMO complications OT - ECMO outcomes OT - cannulation OT - congenital diaphragmatic hernia OT - extracorporeal membrane oxygenation OT - surgical repair OT - surgical timing OT - veno-arterial ECMO OT - veno-venous ECMO COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:37 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - children13101417 [pii] AID - children-13-01417 [pii] AID - 10.3390/children13101417 [doi] PST - epublish SO - Children (Basel). 2026 Oct 15;13(10):1417. doi: 10.3390/children13101417. PMID- 42940732 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Oct 15 TI - Telemedicine in Retinopathy of Prematurity: A Review of Current Evidence and Clinical Outcomes. LID - 10.3390/children13101413 [doi] LID - 1413 AB - Background/Objectives: Retinopathy of prematurity (ROP) is a leading cause of preventable childhood visual impairment, particularly in settings where access to specialized ophthalmologic care is limited. Telemedicine offers an alternative approach to ROP screening and monitoring through remote retinal imaging and expert interpretation. This narrative review aimed to evaluate the diagnostic performance, clinical benefits, implementation challenges, and future perspectives of telemedicine in ROP screening and management. Methods: A narrative literature review was conducted with consideration of the Scale for the Assessment of Narrative Review Articles (SANRA) criteria. PubMed, Scopus, and Google Scholar were searched in August and August 2026 for relevant publications from January 2000 to the search date. Two authors independently screened titles and abstracts, followed by full-text assessment according to predefined eligibility criteria. A total of 143 scientific articles and other high-quality sources were included in the final narrative synthesis. Evidence was evaluated qualitatively, without a formal risk-of-bias assessment. Findings: The reviewed literature indicates that telemedicine can expand access to ROP screening, facilitate remote specialist consultation, support longitudinal disease monitoring, and improve the triage of infants requiring urgent assessment. In selected settings, remote retinal image interpretation has demonstrated diagnostic performance comparable to conventional examinations for clinically significant and vision-threatening ROP. Artificial intelligence-based assessment and smartphone-based retinal imaging represent promising approaches to improving screening accessibility and workflow efficiency, particularly in resource-limited settings. However, implementation remains constrained by equipment costs, infrastructure limitations, image quality, workforce training requirements, and ethical and legal considerations. Conclusions: Telemedicine represents a promising complementary strategy for ROP screening and management, with the potential to improve access to timely specialist assessment and help prevent ROP-related visual impairment. Its successful integration into clinical practice requires sustainable infrastructure, standardized protocols, trained personnel, and reliable referral pathways that ensure timely in-person examination and treatment when indicated. Further research is needed to establish the effectiveness, cost-efficiency, and long-term sustainability of different telemedicine-based approaches across diverse healthcare settings. FAU - Iftime, Ilinca-Medeea AU - Iftime IM AD - Faculty of Medicine, "Grigore T. Popa" University of Medicine and Pharmacy, 16 Universitatii Street, 700115 Iasi, Romania. FAU - Treteanu, Andreea-Ramona AU - Treteanu AR AUID- ORCID: 0009-0006-9232-8063 AD - Department of Pediatrics, "Carol Davila" University of Medicine and Pharmacy, 050474 Bucharest, Romania. AD - Center of Innovation and e-Health, "Carol Davila" University of Medicine and Pharmacy, 030167 Bucharest, Romania. FAU - Puscasu, Andreea AU - Puscasu A AD - Department of Pediatrics, "Carol Davila" University of Medicine and Pharmacy, 050474 Bucharest, Romania. AD - Center of Innovation and e-Health, "Carol Davila" University of Medicine and Pharmacy, 030167 Bucharest, Romania. AD - "Alessandrescu-Rusescu" National Institute for Mother and Child Health, Lacul Tei 120, 020395 Bucharest, Romania. FAU - Craiu, Mihai AU - Craiu M AUID- ORCID: 0000-0001-6194-6797 AD - Department of Pediatrics, "Carol Davila" University of Medicine and Pharmacy, 050474 Bucharest, Romania. AD - "Alessandrescu-Rusescu" National Institute for Mother and Child Health, Lacul Tei 120, 020395 Bucharest, Romania. FAU - Cristea, Octaviana AU - Cristea O AD - Department of Pediatrics, "Carol Davila" University of Medicine and Pharmacy, 050474 Bucharest, Romania. AD - "Alessandrescu-Rusescu" National Institute for Mother and Child Health, Lacul Tei 120, 020395 Bucharest, Romania. FAU - Ciolpan, Alina Angelica AU - Ciolpan AA AD - Department of Pediatrics, "Carol Davila" University of Medicine and Pharmacy, 050474 Bucharest, Romania. AD - "Alessandrescu-Rusescu" National Institute for Mother and Child Health, Lacul Tei 120, 020395 Bucharest, Romania. LA - eng PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703176 OTO - NOTNLM OT - diagnosis OT - management OT - retinopathy of prematurity OT - screening OT - tele-ROP OT - telemedicine COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:37 PHST- 2026/08/25 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - children13101413 [pii] AID - children-13-01413 [pii] AID - 10.3390/children13101413 [doi] PST - epublish SO - Children (Basel). 2026 Oct 15;13(10):1413. doi: 10.3390/children13101413. PMID- 42940725 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Oct 14 TI - Successful Nonsurgical Management of Norovirus-Associated Necrotizing Enterocolitis in a Preterm Infant: A Case Report. LID - 10.3390/children13101406 [doi] LID - 1406 AB - Norovirus infection has been increasingly reported in association with necrotizing enterocolitis (NEC) in preterm neonates, although the clinical spectrum remains poorly defined. We report the case of a 29-week preterm male infant who developed abdominal distension on day 35 of life, with a sepsis-like clinical presentation. Stool reverse transcriptase polymerase chain reaction confirmed Norovirus infection. Serial abdominal radiographs showed intestinal pneumatosis from day 2 of illness, consistent with Bell stage IIA NEC. The pneumatosis persisted for three days and completely resolved by day 5, without perforation or clinical deterioration. The infant was managed conservatively with bowel rest, parenteral nutrition, broad-spectrum antibiotics, and mechanical ventilation for 7 days. IVIG (500 mg/kg/day for 4 days) was administered for clinically suspected capillary leak syndrome. Enteral feeding was restarted after 8 days, and the infant was discharged at 71 days of life without gastrointestinal complications. This case highlights the heterogeneous clinical spectrum of Norovirus-associated intestinal disease and suggests that conservative management may be successful in selected clinically stable preterm infants with NEC and intestinal pneumatosis. FAU - Sessa, Linda AU - Sessa L AUID- ORCID: 0000-0003-0317-2011 AD - Neonatology Unit, Ospedale Policlinico Casilino, 00169 Rome, Italy. FAU - Martini, Lucilla AU - Martini L AD - Neonatology Unit, Ospedale Policlinico Casilino, 00169 Rome, Italy. FAU - Tedesco, Marta AU - Tedesco M AD - Neonatology Unit, Ospedale Policlinico Casilino, 00169 Rome, Italy. FAU - Ferrazza, Alessandro AU - Ferrazza A AD - Radiodiagnostic Unit, Ospedale Policlinico Casilino, 00169 Rome, Italy. FAU - Stefanini, Matteo AU - Stefanini M AD - Radiodiagnostic Unit, Ospedale Policlinico Casilino, 00169 Rome, Italy. FAU - Menzella, Nicoletta AU - Menzella N AUID- ORCID: 0009-0009-8023-167X AD - Department of Woman and Child Health and Public Health, Fondazione Policlinico Universitario Agostino Gemelli, IRCCS-Catholic University of Sacred Heart, 00168 Rome, Italy. FAU - Priolo, Francesca AU - Priolo F AUID- ORCID: 0000-0002-0897-4809 AD - Department of Woman and Child Health and Public Health, Fondazione Policlinico Universitario Agostino Gemelli, IRCCS-Catholic University of Sacred Heart, 00168 Rome, Italy. FAU - Vento, Giovanni AU - Vento G AUID- ORCID: 0000-0003-3121-9569 AD - Department of Woman and Child Health and Public Health, Fondazione Policlinico Universitario Agostino Gemelli, IRCCS-Catholic University of Sacred Heart, 00168 Rome, Italy. FAU - Costa, Simonetta AU - Costa S AUID- ORCID: 0000-0003-0980-5297 AD - Neonatology Unit, Ospedale Policlinico Casilino, 00169 Rome, Italy. LA - eng PT - Case Reports PT - Journal Article DEP - 20261014 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703796 OTO - NOTNLM OT - intestinal pneumatosis OT - intravenous immunoglobulin OT - necrotizing enterocolitis OT - norovirus infection OT - preterm infant COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:37 PHST- 2026/09/12 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - children13101406 [pii] AID - children-13-01406 [pii] AID - 10.3390/children13101406 [doi] PST - epublish SO - Children (Basel). 2026 Oct 14;13(10):1406. doi: 10.3390/children13101406. PMID- 42940723 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Oct 14 TI - Children's Perception of Parental Body Size and Its Association with Their Desire to Be Like the Parent. LID - 10.3390/children13101404 [doi] LID - 1404 AB - BACKGROUND: Childhood obesity is a major and growing public health challenge worldwide. Although parental misperception of children's weight is well documented, how children perceive their parents' body size and whether parental body size is associated with children's expressed desire to be like the parent have received little attention. We examined children's perception of parental body size and whether parental BMI was associated with their expressed desire to be like the parent in a primary care sample. METHODS: This single-center, cross-sectional study included 229 parent-child pairs consecutively recruited from a family medicine outpatient clinic in Turkiye between January and March 2026. Children aged 4-8 years rated the accompanying parent's body size and reported whether they wanted to be like that parent, while parents independently rated their own body size. The BMI-based Body Size Guide was used for all figure selections, and the classification derived from objectively measured parental BMI served as the primary reference. Agreement was evaluated using Cohen's kappa coefficients, associations were examined using multivariable logistic regression, and the children's spontaneous explanations were examined using qualitative content analysis. RESULTS: Parents showed moderate weighted agreement with the BMI-derived classification (weighted kappa = 0.536, 95% CI: 0.456-0.612; exact agreement: 59.4%), whereas children showed fair weighted agreement (weighted kappa = 0.315, 95% CI: 0.231-0.392; exact agreement: 42.8%). After adjustment for child age, child sex, child weight status, parent sex, and parent age, parental overweight (aOR = 0.353, 95% CI: 0.170-0.733) and parental obesity (aOR = 0.133, 95% CI: 0.057-0.307) were associated with lower odds of the child reporting a desire to be like the parent. After adjustment for child age, child sex, parent sex, and parent age, parental overweight (aOR = 2.818, 95% CI: 1.101-7.211) and parental obesity (aOR = 4.774, 95% CI: 1.816-12.546) were associated with higher odds of child overweight/obesity. Among the 53 children who provided an explanation, responses referred to body size, weight, health, appearance, affection, and identification with the parent, illustrating the broad interpretation of the question. CONCLUSIONS: Children aged 4-8 years were able to rate parental body size, although their agreement with the BMI-derived classification was limited. Children's expressed desire to be like the parent varied according to parental BMI; however, this broad question may reflect emotional, relational, behavioural, and appearance-related considerations rather than bodily resemblance alone. The open-ended responses provide context by showing that only some children spontaneously referred to body size or weight. These findings support further research using developmentally validated and stigma-sensitive measures to clarify how young children perceive parental body size and interpret questions about identification with a parent. FAU - Kurt, Abdullah Kaan AU - Kurt AK AUID- ORCID: 0000-0002-6302-6035 AD - Department of Family Medicine, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkiye. FAU - Karakullukcu, Serdar AU - Karakullukcu S AUID- ORCID: 0000-0001-7673-7699 AD - Department of Public Health, Faculty of Medicine, Karadeniz Technical University, 61080 Trabzon, Turkiye. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703376 OTO - NOTNLM OT - body image OT - body size OT - child OT - parent-child relations OT - pediatric obesity COIS- The authors declare that they have no competing interests. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:37 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - children13101404 [pii] AID - children-13-01404 [pii] AID - 10.3390/children13101404 [doi] PST - epublish SO - Children (Basel). 2026 Oct 14;13(10):1404. doi: 10.3390/children13101404. PMID- 42940711 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Oct 13 TI - Implementation Determinants and System-Level Barriers in Supplementary Feeding Programs for Children Under Five in Low- and Middle-Income Countries: A Critical Narrative Review. LID - 10.3390/children13101392 [doi] LID - 1392 AB - Background: Supplementary feeding programs are widely used to prevent or manage child undernutrition, but their population-level benefit depends on implementation. Objective: To critically synthesize multilevel determinants of program reach, continuity, utilization, equity and sustainability in low- and middle-income countries (LMICs). Methods: We conducted a critical narrative review of peer-reviewed and grey literature identified through iterative database searching and targeted verification. Sources were charted by setting, design, program, implementation determinant, consequence, evidence directness, limitation and narrative credibility. Direct, related and indirect evidence were weighted differently in the synthesis. Results: Direct and related evidence most consistently linked program performance to financing and supply continuity, workforce preparation and supervision, food acceptability, caregiver understanding, equitable access and monitoring linked to corrective action. Direct evidence was concentrated in South Asia and selected programs in Indonesia, Iran and Ethiopia. Evidence concerning digital delivery, father-inclusive approaches, community-health-worker incentives and donor transition was largely indirect and supports hypotheses rather than established program effects. Conclusions: Reliable delivery systems and effective child-level utilization are necessary for supplementary feeding programs to contribute to nutritional outcomes. Recommendations supported mainly by indirect evidence require prospective evaluation in supplementary feeding settings, particularly in underrepresented LMIC regions. FAU - Patni, Mohamed Anas AU - Patni MA AUID- ORCID: 0000-0001-6948-3619 AD - Department of Community Medicine, RAK College of Medical Sciences, RAK Medical and Health Sciences University, Ras Al Khaimah 11172, United Arab Emirates. AD - Department of Community Medicine, School of Epidemiology and Public Health, Jawaharlal Nehru Medical College, Adani Institute of Higher Education and Research, Wardha 442107, India. FAU - Choudhari, Sonali G AU - Choudhari SG AUID- ORCID: 0000-0003-0642-5096 AD - Department of Community Medicine, School of Epidemiology and Public Health, Jawaharlal Nehru Medical College, Adani Institute of Higher Education and Research, Wardha 442107, India. FAU - George, Biji Thomas AU - George BT AUID- ORCID: 0000-0001-5029-7779 AD - Department of General Surgery, RAK College of Medical Sciences, RAK Medical and Health Sciences University, Ras Al Khaimah 11172, United Arab Emirates. FAU - Salama, Rasha Aziz Attia AU - Salama RAA AUID- ORCID: 0000-0002-1102-9306 AD - Department of Community Medicine, RAK College of Medical Sciences, RAK Medical and Health Sciences University, Ras Al Khaimah 11172, United Arab Emirates. AD - Department of Community Medicine, Kasr El Aini Faculty of Medicine, Cairo University, Cairo 11956, Egypt. FAU - Bahutair, Shadha Nasser AU - Bahutair SN AUID- ORCID: 0009-0002-6427-1355 AD - Department of Obstetrics & Gynecology, RAK College of Medical Sciences, RAK Medical and Health Sciences University, Ras Al Khaimah 11172, United Arab Emirates. FAU - Akikwala, Mushirabanu Sharifmiyan AU - Akikwala MS AD - Department of Pediatrics, Shifa Al Jazeera Medical Centre, Ras Al Khaimah 11172, United Arab Emirates. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703596 OTO - NOTNLM OT - child undernutrition OT - community health workers OT - equity OT - health systems OT - implementation science OT - low- and middle-income countries OT - program sustainability OT - supplementary feeding COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:37 PHST- 2026/08/30 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - children13101392 [pii] AID - children-13-01392 [pii] AID - 10.3390/children13101392 [doi] PST - epublish SO - Children (Basel). 2026 Oct 13;13(10):1392. doi: 10.3390/children13101392. PMID- 42940699 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Oct 12 TI - Handgrip Strength Is Associated with Standing Long-Jump but Not 20-m Shuttle-Run Performance in Saudi Schoolgirls. LID - 10.3390/children13101380 [doi] LID - 1380 AB - Objective: This study examined the associations between aerobic and muscular fitness and evaluated whether handgrip strength (HGS) could serve as a simple indicator of physical fitness, as determined by physical fitness tests, among Saudi girls aged 10 to 14 years. Methods: Four hundred and fifty-seven girls aged 10 to 14 years from elementary and intermediate schools in Buraidah City, Saudi Arabia, were selected to participate in this cross-sectional study. Anthropometric measurements (height, weight, body composition, and fat percentage) were assessed. Physical fitness tests included HGS, standing long jump, and a 20-m shuttle run. Results: Absolute HGS was positively and significantly correlated with all study variables, except the 20mSRT. Absolute HGS was positively correlated with age, height, weight, BMI, fat percentage, and SLJ performance, but not with 20mSRT performance. In mixed-effects regression models that accounted for clustering by school, age, BMI, and HGS were not significantly associated with 20mSRT performance. In contrast, HGS was independently and positively associated with SLJ performance (B = 2.23 cm per kg HGS, 95% CI 1.62 to 2.84, p < 0.001), whereas age and BMI were negatively associated with SLJ. Similar findings were observed in analyses stratified by elementary and intermediate school levels, in which HGS remained a significant positive predictor of SLJ but not of 20mSRT performance. Conclusions: HGS was independently associated with SLJ but not with 20mSRT performance. These findings indicate that HGS was independently associated with standing long-jump performance among Saudi schoolgirls. FAU - Alhabib, Hessa A AU - Alhabib HA AUID- ORCID: 0009-0004-7801-4143 AD - Department of Food Science and Human Nutrition, College of Agriculture and Food, Qassim University, Buraydah 51452, Saudi Arabia. FAU - Alhussain, Maha H AU - Alhussain MH AUID- ORCID: 0000-0003-0168-6721 AD - Department of Food Sciences and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh 11451, Saudi Arabia. FAU - Qaisar, Rizwan AU - Qaisar R AUID- ORCID: 0000-0001-8485-7172 AD - Department of Basic Medical Sciences, College of Medicine, University of Sharjah, Sharjah 27272, United Arab Emirates. AD - Space Medicine Research Group, Research Institute of Medical and Health Sciences, University of Sharjah, Sharjah 27272, United Arab Emirates. AD - Cardiovascular Research Group, Research Institute of Medical and Health Sciences, University of Sharjah, Sharjah 27272, United Arab Emirates. FAU - Aljuhani, Osama AU - Aljuhani O AUID- ORCID: 0000-0001-5336-1721 AD - Department of Physical Education, College of Sport Sciences and Physical Activity, King Saud University, Riyadh 11451, Saudi Arabia. FAU - Saeed, Khalid AU - Saeed K AUID- ORCID: 0000-0001-9679-683X AD - The In Silico Drug Discovery Group, University of Helsinki, 00170 Helsinki, Finland. FAU - Alshehri, Abdullah G AU - Alshehri AG AD - Biomechanics and Motor Behavior Department, College of Sport Sciences and Physical Activity, King Saud University, Riyadh 11451, Saudi Arabia. FAU - Alkahtani, Shaea A AU - Alkahtani SA AUID- ORCID: 0000-0002-1443-6249 AD - Exercise Physiology Department, College of Sport Sciences and Physical Activity, King Saud University, Riyadh 11451, Saudi Arabia. LA - eng GR - ORF-2026- 338/King Saud University/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703414 OTO - NOTNLM OT - Saudi girls OT - children OT - handgrip strength OT - physical fitness OT - standing long jump COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:37 PHST- 2026/08/29 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - children13101380 [pii] AID - children-13-01380 [pii] AID - 10.3390/children13101380 [doi] PST - epublish SO - Children (Basel). 2026 Oct 12;13(10):1380. doi: 10.3390/children13101380. PMID- 42940695 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Oct 10 TI - Tear-Film Stability and Ocular Symptoms During Two Years of Low-Dose Atropine Treatment in Children: A Secondary Analysis of the ASPECT Trial. LID - 10.3390/children13101376 [doi] LID - 1376 AB - Background/Objectives: Long-term tear-film and symptom data for compounded 0.025% atropine remain limited in European children. We assessed changes in first non-invasive tear-film break-up time (fNIBUT) and Ocular Surface Disease Index (OSDI) scores over 24 months. Methods: This secondary analysis of the ASPECT trial included 101 children completing 24 months, all assigned nightly compounded 0.025% atropine with Defocus Incorporated Multiple Segments or single-vision spectacles. Available fNIBUT, OSDI, and corneal endothelial cell density (ECD) measurements at baseline, 12 months, and 24 months were analysed using longitudinal mixed-effects models. The six pairwise contrasts across the primary outcomes of this secondary analysis used Holm-adjusted p-values and simultaneous Bonferroni 95% confidence intervals (CIs). No untreated comparator was available. Results: fNIBUT was numerically higher at 24 months than at baseline (16.9 vs. 14.3 s; mean difference [MD], 2.60 s; 95% CI, -0.37 to 5.57; p = 0.103). OSDI decreased from 14.7 to 9.6 (MD, -5.15 points; 95% CI, -9.93 to -0.36; p = 0.028). This reduction was not statistically significant in all examined missing-not-at-random scenarios or in an analysis restricted to children aged >/=8 years. ECD showed no statistically significant longitudinal changes. The exploratory combined criterion of OSDI >/= 13 and fNIBUT < 10 s decreased numerically from 13/98 (13.3%) to 5/86 (5.8%). Conclusions: No deterioration was detected in the measured parameters among 24-month completers. Selection of completers, differential missingness, limited paediatric symptom assessment, and absence of a concurrent untreated comparator preclude firm conclusions about long-term ocular surface tolerability. FAU - Martin-Garcia, Beatriz AU - Martin-Garcia B AUID- ORCID: 0000-0002-5238-315X AD - Department of Optometry and Vision, Faculty of Optics and Optometry, Complutense University of Madrid, 28037 Madrid, Spain. FAU - Montolio-Marzo, Elena AU - Montolio-Marzo E AUID- ORCID: 0000-0001-6727-7432 AD - Department of Ophthalmology, Hospital Clinico San Carlos, 28040 Madrid, Spain. AD - Health Research Institute of Hospital Clinico San Carlos (IdISSC), Biomedical Research Foundation of Hospital Clinico San Carlos (FIBHCSC), 28040 Madrid, Spain. FAU - Guemes-Villahoz, Noemi AU - Guemes-Villahoz N AUID- ORCID: 0000-0002-9289-1212 AD - Department of Ophthalmology, Hospital Clinico San Carlos, 28040 Madrid, Spain. AD - Health Research Institute of Hospital Clinico San Carlos (IdISSC), Biomedical Research Foundation of Hospital Clinico San Carlos (FIBHCSC), 28040 Madrid, Spain. AD - Ramon Castroviejo Institute for Ophthalmic Research (IIORC), Department of Immunology, Ophthalmology and Otorhinolaryngology, Faculty of Medicine, Complutense University of Madrid, 28040 Madrid, Spain. FAU - Alonso-Abad, Blanca AU - Alonso-Abad B AD - Department of Optometry and Vision, Faculty of Optics and Optometry, Complutense University of Madrid, 28037 Madrid, Spain. AD - Department of Ophthalmology, Hospital Clinico San Carlos, 28040 Madrid, Spain. AD - Health Research Institute of Hospital Clinico San Carlos (IdISSC), Biomedical Research Foundation of Hospital Clinico San Carlos (FIBHCSC), 28040 Madrid, Spain. FAU - Ruiz-Pomeda, Alicia AU - Ruiz-Pomeda A AUID- ORCID: 0000-0002-8318-5914 AD - Department of Optometry and Vision, Faculty of Optics and Optometry, Complutense University of Madrid, 28037 Madrid, Spain. FAU - Talavero-Gonzalez, Paula AU - Talavero-Gonzalez P AD - Department of Ophthalmology, Hospital Clinico San Carlos, 28040 Madrid, Spain. AD - Health Research Institute of Hospital Clinico San Carlos (IdISSC), Biomedical Research Foundation of Hospital Clinico San Carlos (FIBHCSC), 28040 Madrid, Spain. FAU - Hernandez-Garcia, Elena AU - Hernandez-Garcia E AD - Department of Ophthalmology, Hospital Clinico San Carlos, 28040 Madrid, Spain. AD - Health Research Institute of Hospital Clinico San Carlos (IdISSC), Biomedical Research Foundation of Hospital Clinico San Carlos (FIBHCSC), 28040 Madrid, Spain. FAU - Bella-Gala, Rafael AU - Bella-Gala R AUID- ORCID: 0000-0003-1469-718X AD - Department of Optometry and Vision, Faculty of Optics and Optometry, Complutense University of Madrid, 28037 Madrid, Spain. FAU - Gomez de Liano, Nunila AU - Gomez de Liano N AUID- ORCID: 0009-0008-8341-1599 AD - Department of Optometry and Vision, Faculty of Optics and Optometry, Complutense University of Madrid, 28037 Madrid, Spain. FAU - Simbana-Fierro, Joel AU - Simbana-Fierro J AD - Research Methodological Support Unit of Preventive Department, Instituto de Investigacion Sanitaria del Hospital Clinico San Carlos (IdISSC), 28040 Madrid, Spain. FAU - Garcia-Feijoo, Julian AU - Garcia-Feijoo J AD - Department of Ophthalmology, Hospital Clinico San Carlos, 28040 Madrid, Spain. AD - Health Research Institute of Hospital Clinico San Carlos (IdISSC), Biomedical Research Foundation of Hospital Clinico San Carlos (FIBHCSC), 28040 Madrid, Spain. AD - Ramon Castroviejo Institute for Ophthalmic Research (IIORC), Department of Immunology, Ophthalmology and Otorhinolaryngology, Faculty of Medicine, Complutense University of Madrid, 28040 Madrid, Spain. FAU - Gomez-de-Liano, Rosario AU - Gomez-de-Liano R AUID- ORCID: 0000-0001-6952-4521 AD - Department of Ophthalmology, Hospital Clinico San Carlos, 28040 Madrid, Spain. AD - Health Research Institute of Hospital Clinico San Carlos (IdISSC), Biomedical Research Foundation of Hospital Clinico San Carlos (FIBHCSC), 28040 Madrid, Spain. AD - Ramon Castroviejo Institute for Ophthalmic Research (IIORC), Department of Immunology, Ophthalmology and Otorhinolaryngology, Faculty of Medicine, Complutense University of Madrid, 28040 Madrid, Spain. LA - eng GR - Hoya (Japan)/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13702968 OTO - NOTNLM OT - OSDI OT - childhood myopia OT - compounded formulation OT - fNIBUT OT - low-concentration atropine OT - ocular symptoms OT - tear-film stability OT - tolerability COIS- J.S.-F., A.R.-P., B.M.-G., E.M.-M., and N.G.d.L. declare no competing interests. B.A.-A. has received honoraria for lectures and/or educational activities from companies involved in myopia management outside the submitted work. N.G.-V., R.G.-d.-L., E.H.-G., P.T.-G., R.B.-G., and J.G.-F. have received honoraria for lectures, educational activities, advisory board participation, research funding, and/or research collaborations from HOYA Vision Care and/or other companies involved in myopia management outside the submitted work. The other authors declare that they have no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:37 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - children13101376 [pii] AID - children-13-01376 [pii] AID - 10.3390/children13101376 [doi] PST - epublish SO - Children (Basel). 2026 Oct 10;13(10):1376. doi: 10.3390/children13101376. PMID- 42940694 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Oct 10 TI - Pen-Based Graphomotor Measures for Early Identification of Attention-Related Difficulties in Preschool Children: A Conceptual and Critical Narrative Review. LID - 10.3390/children13101375 [doi] LID - 1375 AB - Attention problems may compromise child development, learning, behavioral regulation, and school adjustment. The preschool years are an important period for early identification because attentional control develops rapidly and supports the transition to formal schooling. Existing assessment approaches, however, provide limited information about how attentional regulation unfolds during ongoing behavior. This non-systematic narrative review synthesizes evidence from preschool attention assessment, graphomotor development, digital handwriting, and measurement theory using a targeted, concept-driven approach. No systematic literature search or formal study-selection protocol was employed. It proposes pen-based graphomotor attention measurement (PGAM) as a measurement-development framework for examining how children select relevant information, maintain task goals, monitor movement, and correct deviations during graphomotor activity. PGAM links theoretically specified attentional demands and continuous pen-trajectory data to dynamic patterns of engagement, maintenance, disruption, and recovery during goal-directed behavior. Available evidence supports the technical feasibility of digital graphomotor recording and suggests associations between attention-related functioning and graphomotor organization. Future studies should first establish the reliability and developmental calibration of PGAM. Subsequent work should evaluate construct and incremental validity and decision utility to determine whether these process measures can support attention-specific interpretation and potential screening use. FAU - Ji, Muxian AU - Ji M AUID- ORCID: 0009-0000-0573-1214 AD - Bay Area School of Applied Psychological Sciences, Beijing Normal University at Zhuhai, Zhuhai 519087, China. AD - Faculty of Psychology, Beijing Normal University, Beijing 100875, China. FAU - Du, Tingshu AU - Du T AD - Bay Area School of Applied Psychological Sciences, Beijing Normal University at Zhuhai, Zhuhai 519087, China. AD - Faculty of Psychology, Beijing Normal University, Beijing 100875, China. FAU - Ma, Tingting AU - Ma T AD - Bay Area School of Applied Psychological Sciences, Beijing Normal University at Zhuhai, Zhuhai 519087, China. AD - Faculty of Psychology, Beijing Normal University, Beijing 100875, China. FAU - Hu, Luming AU - Hu L AUID- ORCID: 0000-0003-3604-4905 AD - Bay Area School of Applied Psychological Sciences, Beijing Normal University at Zhuhai, Zhuhai 519087, China. AD - Faculty of Psychology, Beijing Normal University, Beijing 100875, China. LA - eng GR - 2024KQNCX156/the Young Innovative Talents Project for General Universities of Guangdong Province/ GR - 62607009/National Natural Science Foundation of China/ PT - Journal Article PT - Review DEP - 20261010 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703223 OTO - NOTNLM OT - attention OT - digital handwriting OT - early identification OT - graphomotor assessment OT - narrative review OT - preschool children COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:37 PHST- 2026/07/29 00:00 [received] PHST- 2026/09/06 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - children13101375 [pii] AID - children-13-01375 [pii] AID - 10.3390/children13101375 [doi] PST - epublish SO - Children (Basel). 2026 Oct 10;13(10):1375. doi: 10.3390/children13101375. PMID- 42940691 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Oct 10 TI - Exploring Caregivers Lived Experiences in Accessing Comprehensive Cleft Care at Jimma University Medical Center: A Phenomenological Study in Southwest Ethiopia. LID - 10.3390/children13101372 [doi] LID - 1372 AB - Background: Orofacial clefts are one of the most common congenital anomalies worldwide. Comprehensive cleft care interventions aim to address the complex surgical, nutritional, dental, speech, audiology, and psychosocial needs of patients with clefts. While studies from other countries have identified various implementation barriers and facilitators, evidence from Ethiopia remains limited, particularly regarding caregivers' experiences accessing comprehensive cleft care. This study explored caregivers' lived experiences of accessing comprehensive cleft care at Jimma University Medical Center in southwestern Ethiopia. Methods: We conducted a phenomenological study at the Comprehensive Cleft Care Center of Jimma University Medical Center. Participants were purposively selected from caregivers of children with orofacial clefts who had received at least two components of comprehensive cleft care. Data were collected through in-depth semi-structured interviews. Data collection continued until thematic saturation was achieved. Audio recordings were transcribed verbatim, translated into English, and managed in ATLAS.ti version 7.5.18. Data were analyzed inductively using Colaizzi's seven-step descriptive phenomenological method. Trustworthiness was enhanced through member checking, reflexive bracketing, and systematic procedures. Results: Twelve caregivers of children with orofacial clefts participated in the study. The analysis generated eight overarching themes. Participants reported limited initial awareness of cleft conditions, cultural misconceptions, stigma, emotional distress, and delayed care-seeking. Key barriers included poor referral linkages, transportation difficulties, financial constraints, and geographic inaccessibility. Participants reported that engagement with comprehensive cleft care was associated with increased knowledge, caregiving confidence and skills, and trust in healthcare providers. They also described perceived improvements in feeding, nutritional status, speech development, physical appearance, and social integration, alongside reduced stigma and increased caregiver advocacy and community awareness of cleft conditions. Conclusions: Caregivers described a transition from limited awareness of orofacial clefts, emotional distress, stigma, and challenges accessing care toward greater knowledge, confidence, trust in healthcare providers, and perceived improvements in their children's health, functional abilities, and social participation. Persistent barriers related to poverty, transportation difficulties, referral delays, and limited community awareness remained. These findings can inform policy and program planning to strengthen comprehensive cleft care and address inequities in access to care for children with orofacial clefts and their families in similar settings in Ethiopia. FAU - Dejene, Abiy Semunigus AU - Dejene AS AD - Department of Health, Behavior, and Society, Faculty of Public Health, Institute of Health, Jimma University, Jimma P.O. Box 378, Ethiopia. FAU - Auslander, Allyn AU - Auslander A AD - Operation Smile, Virginia Beach, VA 23453, USA. FAU - Gizaw, Abraham Tamirat AU - Gizaw AT AD - Department of Health, Behavior, and Society, Faculty of Public Health, Institute of Health, Jimma University, Jimma P.O. Box 378, Ethiopia. FAU - Ababulgu, Fira Abamecha AU - Ababulgu FA AUID- ORCID: 0000-0002-8311-5609 AD - Department of Health, Behavior, and Society, Faculty of Public Health, Institute of Health, Jimma University, Jimma P.O. Box 378, Ethiopia. FAU - Yesuf, Elias Ali AU - Yesuf EA AUID- ORCID: 0009-0004-0323-5208 AD - Department of Health Policy and Management, Faculty of Public Health, Institute of Health, Jimma University, Jimma P.O. Box 378, Ethiopia. LA - eng PT - Journal Article DEP - 20261010 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703069 OTO - NOTNLM OT - comprehensive cleft care OT - lived experience OT - orofacial clefts OT - phenomenological study COIS- The authors declare no conflicts of interest. The primary researcher and one co-author are staff of Operation Smile. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:37 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - children13101372 [pii] AID - children-13-01372 [pii] AID - 10.3390/children13101372 [doi] PST - epublish SO - Children (Basel). 2026 Oct 10;13(10):1372. doi: 10.3390/children13101372. PMID- 42940686 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Oct 8 TI - Association of Regular Piano Training with Dental Anxiety and Stress in Children Undergoing Pit and Fissure Sealant Application: A Cross-Sectional Study. LID - 10.3390/children13101367 [doi] LID - 1367 AB - Background/Objectives: Regular musical training has been associated with emotional regulation and stress coping, but its relationship with children's dental responses remains unclear. This study evaluated associations between regular piano training and dental anxiety, perceived stress, and physiological responses during fissure sealant application. Methods: Eighty systemically healthy children aged 7-11 years were categorized as a piano group (PG; n = 40) or control group (CG; n = 40) based on pre-existing piano training. All underwent standardized fissure sealant application using Tell-Show-Do. Dental anxiety and perceived stress were assessed before and after treatment using MCDAS(f), FIS, physiological parameters, and PeSSKi. Results: Pre-treatment MCDAS(f) scores did not differ between groups, whereas post-treatment scores were lower in the PG. Overall FIS scores were lower in the PG. PeSSKi scores were lower in the PG at T1 and remained lower at T2, indicating a between-group difference already present before the dental procedure. Significant group x time interactions were observed for heart rate, systolic blood pressure, and MCDAS(f). Post-treatment MCDAS(f), FIS, and PeSSKi differences remained significant after adjustment for baseline scores and parental education. In exploratory within-group analyses controlling for age, longer formal piano education was associated with lower PeSSKi scores at T1 and T2, while greater weekly formal piano lesson duration was associated with lower post-treatment MCDAS(f) and PeSSKi scores. These p values were unadjusted for multiple comparisons and should therefore be interpreted cautiously. Conclusions: Regular piano training was associated with lower dental anxiety and perceived stress and selected physiological responses during fissure sealant application. FAU - Ural, Eda Nur AU - Ural EN AD - Department of Pediatric Dentistry, Faculty of Dentistry, Recep Tayyip Erdogan University, Rize 53020, Turkiye. FAU - Aydinoglu, Sema AU - Aydinoglu S AD - Department of Pediatric Dentistry, Faculty of Dentistry, Recep Tayyip Erdogan University, Rize 53020, Turkiye. FAU - Gunacar, Dilara Nil AU - Gunacar DN AUID- ORCID: 0000-0002-9607-6362 AD - Department of Oral and Maxillofacial, Radiology, Faculty of Dentistry, Recep Tayyip Erdogan University, Rize 53020, Turkiye. LA - eng PT - Journal Article DEP - 20261008 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703332 OTO - NOTNLM OT - dental anxiety OT - music education OT - pediatric dentistry OT - stress COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:37 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - children13101367 [pii] AID - children-13-01367 [pii] AID - 10.3390/children13101367 [doi] PST - epublish SO - Children (Basel). 2026 Oct 8;13(10):1367. doi: 10.3390/children13101367. PMID- 42940685 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Oct 8 TI - Outdoor Environmental Risk Factors for Paediatric Ragweed Sensitisation: A Formal Model Comparison. LID - 10.3390/children13101366 [doi] LID - 1366 AB - Background/Objectives: Ragweed (Ambrosia artemisiifolia) is a major aeroallergen in Central and Eastern Europe. Croatian children in regions with twofold difference in pollen exposure show near-identical sensitisation prevalence (the "Zagreb paradox"), and an earlier analysis found regional pollen and rural residence to be the only associated environmental factors. We re-examined whether air pollution, surrounding landscape and indoor environment contribute to it. Methods: This cross-sectional analysis used 3520 of the 4016 children recruited to the EU FP7 ATOPICA study, who had their complete data re-analysed within the EU Horizon Europe EDIAQI project. Sensitisation was defined as a skin prick test wheal of at least 2 mm. Environmental predictors (particulate matter, PM(10); nitrogen dioxide, NO(2); regional pollen; CORINE land cover within 5 km; rural residence; and indoor exposures) were compared using the Akaike Information Criterion (AIC) in logistic regression models, which were adjusted for age and parental atopy. Results: Sensitisation prevalence was 10.8%. PM(10) and categorical pollen area fitted the data equally well (DeltaAIC 1.7): both describe the same inland-coastal contrast and cannot be distinguished with area-level data. NO(2) added nothing to either. Of the 16 landscape variables, only transitional woodland-shrub remained associated with sensitisation after Bonferroni correction (adjusted odds ratio, aOR 0.93 [95% confidence interval, CI 0.90-0.97]); total green area was nominally associated (aOR 0.92 [95% CI 0.87-0.98]; p = 0.009). Children in rural locations were less often sensitised (aOR 0.69 [95% CI 0.50-0.96]; p = 0.026), as previously reported; a mediation analysis could not determine whether broadleaved forest cover explains this association. Indoor exposures added little to model fit. Conclusions: Area-level data cannot separate particulate air pollution from pollen exposure, so a contribution of air pollution can be neither confirmed nor excluded. Rural residence and, with less certainty, surrounding green space were associated with less sensitisation, and the indoor environment added little to it. FAU - Lovric, Mario AU - Lovric M AUID- ORCID: 0000-0002-3541-9624 AD - The Lisbon Council, 1040 Brussels, Belgium. AD - Institute for Anthropological Research, 10000 Zagreb, Croatia. FAU - Banic, Ivana AU - Banic I AUID- ORCID: 0000-0002-0017-142X AD - Srebrnjak Children's Hospital, 10000 Zagreb, Croatia. FAU - Casni, Kristina AU - Casni K AUID- ORCID: 0000-0001-6069-7020 AD - Know Center Research GmbH, 8010 Graz, Austria. FAU - Sunic, Iva AU - Sunic I AUID- ORCID: 0000-0002-5289-9463 AD - The Lisbon Council, 1040 Brussels, Belgium. FAU - Jerkovic, Ivana AU - Jerkovic I AD - Srebrnjak Children's Hospital, 10000 Zagreb, Croatia. FAU - Turkalj, Mirjana AU - Turkalj M AUID- ORCID: 0000-0002-5339-861X AD - Srebrnjak Children's Hospital, 10000 Zagreb, Croatia. AD - Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, 31000 Osijek, Croatia. AD - Faculty of Medicine, Catholic University of Croatia, 10000 Zagreb, Croatia. LA - eng GR - 101057497/Council of Europe/ PT - Journal Article DEP - 20261008 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13702990 OTO - NOTNLM OT - air pollution OT - green spaces OT - model selection OT - paediatric allergy OT - ragweed sensitisation COIS- Author Kristina Casni was employed by the company Know Center Research GmbH. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:37 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - children13101366 [pii] AID - children-13-01366 [pii] AID - 10.3390/children13101366 [doi] PST - epublish SO - Children (Basel). 2026 Oct 8;13(10):1366. doi: 10.3390/children13101366. PMID- 42940684 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Oct 8 TI - Understanding Cognitive Heterogeneity in Children Referred to Mental Health Services: Evidence from a Large Clinical Sample. LID - 10.3390/children13101365 [doi] LID - 1365 AB - Background: General ability and verbal-performance organisation are established features of Wechsler batteries, while the diagnostic value of subtest profile types remains contested. We examined whether ten WISC-R subtests in a large clinical referral sample contained reproducible profile-based groups beyond the overall cognitive level. Methods: Ten WISC-R subtests from 1261 children in a single-centre child psychiatry archive were analysed using principal components, parallel analysis, an orthogonal bifactor model, Gaussian mixtures with multiple covariance structures, k-means, level-free profile analyses, and 500 subsample stability replications. Results: The first component accounted for 50.4% of the variance and correlated with Full-Scale IQ (r = 0.997). The second accounted for 11.0% and closely tracked PIQ-VIQ (r = 0.942); the parallel analysis retained only the first component. A bifactor model showed a strong general factor (omega-hierarchical = 0.814; explained common variance = 0.767). Raw-score k-means produced a reproducible higher-versus-lower level split (mean Full-Scale IQ 101.4 vs. 71.6; silhouette = 0.261), but, after removing the general level, the full- and equal-covariance mixture models selected one class, and forced k-means solutions had silhouettes of 0.08-0.12. Conclusions: Within the ten WISC-R variables examined, no clearly separated profile-based subtypes were detected beyond graded differences in the general level and verbal-performance balance. These findings concern the internal structure of one battery in one clinical referral sample and do not establish the absence of clinically meaningful subtypes defined by broader functional measures. FAU - Ozkan, Selcuk AU - Ozkan S AUID- ORCID: 0000-0002-7370-1524 AD - Department of Child and Adolescent Psychiatry, Faculty of Medicine, Kutahya Health Sciences University, Kutahya 43100, Turkiye. FAU - Ayaz, Abdullah AU - Ayaz A AUID- ORCID: 0000-0003-1114-9321 AD - Department of Child and Adolescent Psychiatry, Faculty of Medicine, Kutahya Health Sciences University, Kutahya 43100, Turkiye. LA - eng PT - Journal Article DEP - 20261008 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703743 OTO - NOTNLM OT - child and adolescent psychiatry OT - clinical sample OT - clustering OT - cognitive assessment OT - cognitive heterogeneity OT - neurodevelopment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:37 PHST- 2026/09/07 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - children13101365 [pii] AID - children-13-01365 [pii] AID - 10.3390/children13101365 [doi] PST - epublish SO - Children (Basel). 2026 Oct 8;13(10):1365. doi: 10.3390/children13101365. PMID- 42940679 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Oct 8 TI - Claims-Based Diagnostic Registration Patterns and Clinical Burden of Selected Common Aneuploidies in Korean Children: A Nationwide HIRA Study (2009-2023). LID - 10.3390/children13101360 [doi] LID - 1360 AB - BACKGROUND: Selected common aneuploidies, including Down, Edwards, Patau, Turner, and Klinefelter syndromes, are important causes of congenital anomalies and diverse clinical morbidities. However, nationwide evidence regarding the recent diagnostic registration patterns, clinical outcomes, and healthcare burdens in South Korea remains limited. METHODS: We conducted a nationwide descriptive analysis using claims data from the Health Insurance Review and Assessment Service (HIRA) of South Korea between 2009 and 2023. Among individuals aged 0-14 years, we evaluated period-based administrative registration frequency and ratios relative to live births, age at diagnosis, comorbidities including congenital heart disease (CHD) and epilepsy, in-hospital mortality, and first-year healthcare expenditures following the first observed claims-code date. RESULTS: Among the 5,586,770 live births over the 15-year study period, 5900 individuals with one of the five selected common aneuploidies were identified in the HIRA database, corresponding to an overall claims-based diagnostic registration ratio of 105.6 per 100,000 live births. Down syndrome was the most frequently registered disorder, accounting for 59.51% of the cases, followed by Turner syndrome (19.56%), Klinefelter syndrome (14.76%), Edwards syndrome (4.63%), and Patau syndrome (1.54%). The corresponding claims-based diagnostic registration ratios were 62.8, 20.7, 15.6, 4.9, and 1.6 per 100,000 live births, respectively. Edwards and Patau syndromes had the highest clinical burden, with discharge-recorded in-hospital mortality of 34.07% (95% CI, 28.46-40.02) and 32.97% (95% CI, 23.47-43.61), respectively, and the highest median first-year healthcare expenditures of 17,394,435 KRW (Q1-Q3, 1,087,560-61,040,440) and 7,478,665 KRW (Q1-Q3, 510,510-37,524,080), respectively. The prevalence of CHD ranged from 60.7% to 70.3% in Down, Edwards, and Patau syndromes and was approximately 11% in Turner and Klinefelter syndromes. CONCLUSIONS: This nationwide claims-based study demonstrated distinct diagnostic registration patterns and outcome disparities among the selected common aneuploidies in Korean children. These findings support the need for disorder-specific clinical pathways, integrated national surveillance, and targeted supportive care to address the substantial clinical and economic burdens. FAU - Kwon, Hye Eun AU - Kwon HE AD - Department of Pediatrics, Gachon University Gil Medical Center, Incheon 21565, Republic of Korea. FAU - Gwon, So Hyeon AU - Gwon SH AUID- ORCID: 0009-0000-6591-8291 AD - Department of Statistics, College of Natural Science, Keimyung University, Daegu 42601, Republic of Korea. FAU - Son, Nak-Hoon AU - Son NH AD - Department of Statistics, College of Natural Science, Keimyung University, Daegu 42601, Republic of Korea. FAU - Byun, Jun Chul AU - Byun JC AUID- ORCID: 0000-0001-5600-6282 AD - Department of Pediatrics, Keimyung University School of Medicine, Daegu 42601, Republic of Korea. LA - eng PT - Journal Article DEP - 20261008 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703676 OTO - NOTNLM OT - child OT - chromosome disorders OT - diagnostic registration OT - epidemiology OT - healthcare costs COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:37 PHST- 2026/08/11 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - children13101360 [pii] AID - children-13-01360 [pii] AID - 10.3390/children13101360 [doi] PST - epublish SO - Children (Basel). 2026 Oct 8;13(10):1360. doi: 10.3390/children13101360. PMID- 42940670 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Oct 5 TI - Doxapram in Preterm Infants: From Respiratory Stimulation to Individualized Therapy-A Narrative Review. LID - 10.3390/children13101351 [doi] LID - 1351 AB - Background: Apnea of prematurity (AOP) remains one of the most common complications of preterm birth, and despite the established role of caffeine as first-line therapy, a proportion of extremely preterm infants continue to experience clinically significant apnea. Doxapram, a respiratory stimulant first investigated in preterm infants in the 1980s, has remained a potential second-line pharmacological option for more than four decades, yet its place in neonatal practice remains uncertain, with marked variability in use across countries and institutions. Methods: Previous reviews have primarily focused on summarizing the efficacy and safety evidence for doxapram. This narrative review instead examines how knowledge of doxapram's efficacy, safety, pharmacokinetics, and clinical use has evolved over time, situating this evidence within the broader historical transformation of neonatal intensive care. Results: We found that the persistent uncertainty surrounding doxapram reflects not only well-recognized methodological limitations of the available studies, but also the fact that the clinical questions considered relevant have changed more rapidly than the evidence generated to answer them. Conclusions: Doxapram has a relatively consistent short-term respiratory stimulant effect, but evidence that it improves clinically important outcomes remains limited and of low certainty. It should not be regarded as an established therapy for routine use; rather, it may represent a rescue or adjunctive option for selected preterm infants with persistent clinically significant apnea despite optimized caffeine therapy and non-invasive respiratory support. Further adequately powered trials are needed to define its clinical role. FAU - Fazi, Camilla AU - Fazi C AD - Department of Neurosciences, Psychology, Drug Research and Child Health, University of Florence, 50134 Florence, Italy. AD - Division of Neonatology, Careggi University Hospital of Florence, Largo Brambilla 3, 50141 Florence, Italy. FAU - Dani, Carlo AU - Dani C AUID- ORCID: 0000-0002-1639-621X AD - Department of Neurosciences, Psychology, Drug Research and Child Health, University of Florence, 50134 Florence, Italy. AD - Division of Neonatology, Careggi University Hospital of Florence, Largo Brambilla 3, 50141 Florence, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261005 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703389 OTO - NOTNLM OT - apnea of prematurity OT - doxapram OT - preterm infant COIS- C.D. received honoraria from Zoll Medical Inc., Gepa Srl, and Sanofi Italia for scientific consultancy unrelated to the present research. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/05 CRDT- 2026/10/27 10:37 PHST- 2026/08/27 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/05 00:00 [pmc-release] AID - children13101351 [pii] AID - children-13-01351 [pii] AID - 10.3390/children13101351 [doi] PST - epublish SO - Children (Basel). 2026 Oct 5;13(10):1351. doi: 10.3390/children13101351. PMID- 42940665 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Oct 3 TI - Early Extubation After Pediatric Liver Transplantation: From Baseline Vulnerability to Perioperative Readiness. LID - 10.3390/children13101346 [doi] LID - 1346 AB - Early extubation after pediatric liver transplantation (PLTx) is increasingly incorporated into enhanced-recovery pathways, yet definitions, selection criteria, and outcome reporting remain inconsistent. This narrative review synthesizes evidence identified through PubMed/MEDLINE (January 2000 through August 2026), citation screening, and supplementary screening of recent reports. Immediate extubation denotes tracheal extubation in the operating room, whereas early extubation has been defined through 24 postoperative hours; delayed extubation and prolonged mechanical ventilation are similarly heterogeneous. The available evidence informs a dynamic framework in which preoperative characteristics define baseline vulnerability, the intraoperative course reflects whether that vulnerability has been overcome or amplified, and the early postoperative course validates the decision. Young age, low body weight, growth failure, or higher disease-severity scores may increase risk but should not function as absolute exclusions; conversely, low scores may underestimate disease-specific risk in metabolic disorders. Direct readiness indicators include effective ventilation, neurological and quantitative neuromuscular recovery, normothermia, stable hemodynamics without escalating vasoactive support, controlled bleeding, satisfactory abdominal closure, and improving acid-base status and lactate clearance. Early extubation is associated with shorter intensive-care and hospital stays in selected cohorts, but causality remains uncertain because evidence is predominantly retrospective and single-center. Standardized definitions and prospective multicenter validation are required. FAU - Kowdley, Devan AU - Kowdley D AD - School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA. AD - Department of Anesthesiology, Perioperative and Pain Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA. FAU - Martucci, Gennaro AU - Martucci G AUID- ORCID: 0000-0001-8443-2414 AD - Department of Anesthesia and Intensive Care, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), 90127 Palermo, Italy. FAU - Sottani, Valeria AU - Sottani V AUID- ORCID: 0009-0002-4250-1375 AD - Department of Anesthesia and Intensive Care, Faculty of Medicine, University of Palermo, 90127 Palermo, Italy. FAU - Burgio, Gaetano AU - Burgio G AUID- ORCID: 0000-0001-7294-7613 AD - Department of Anesthesia and Intensive Care, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), 90127 Palermo, Italy. FAU - Damian, Daniela AU - Damian D AUID- ORCID: 0000-0001-6935-5043 AD - School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA. AD - Department of Anesthesiology and Perioperative Medicine, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA. AD - Department of Anesthesiology and Perioperative Medicine, UPMC Children's Hospital of Pittsburgh, 4401 Penn Ave, Pittsburgh, PA 15224, USA. LA - eng PT - Journal Article PT - Review DEP - 20261003 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703641 OTO - NOTNLM OT - analgesia OT - early extubation OT - enhanced recovery OT - graft function OT - hemodynamics OT - pediatric liver transplantation OT - transfusion COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/03 CRDT- 2026/10/27 10:37 PHST- 2026/09/03 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - children13101346 [pii] AID - children-13-01346 [pii] AID - 10.3390/children13101346 [doi] PST - epublish SO - Children (Basel). 2026 Oct 3;13(10):1346. doi: 10.3390/children13101346. PMID- 42940659 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Oct 2 TI - Maxillary Labial Frenulum Morphology and Maxillary Anterior Dental Caries in Children: A Scoping Review. LID - 10.3390/children13101340 [doi] LID - 1340 AB - Background/Objectives: The relationship between maxillary labial frenulum (MLF) morphology and caries of the adjacent maxillary anterior teeth remains uncertain. This scoping review mapped the available evidence in children. Methods: Following Joanna Briggs Institute (JBI) guidance and PRISMA-ScR, PubMed/MEDLINE, Scopus, Web of Science, and TR Dizin were searched up to August 2026, plus reference lists. Analytical studies in participants under 18 years of age were eligible. Two reviewers independently screened records and verified charted data; included studies were appraised post hoc using the JBI analytical cross-sectional checklist. Results: Of 68 unique records, six cross-sectional studies (1659 participants aged 6 months-16 years) were included. Five used the Placek classification or an equivalent and one the Kotlow scale; caries outcomes ranged from approximal lesions to ICDAS-based severity. Three studies reported bivariate differences without a consistent gradient, whereas three found no significant association. In the only adjusted analysis, no attachment category differed significantly from the mucosal reference; the others were unadjusted. Conclusions: Evidence is limited and heterogeneous. MLF attachment should not be used as a stand-alone caries risk marker. Current evidence does not support recommending frenectomy solely for the purpose of preventing maxillary anterior caries; this reflects insufficient evidence of benefit rather than evidence of ineffectiveness. Prospective studies with standardized anatomical and functional assessments are needed. FAU - Tuna, Mehmet Ali AU - Tuna MA AUID- ORCID: 0009-0008-5743-0593 AD - Department of Periodontology, Faculty of Dentistry, Ataturk University, 25240 Erzurum, Turkiye. FAU - Naderloo, Faezeh AU - Naderloo F AUID- ORCID: 0009-0003-7190-0700 AD - Department of Periodontology, Faculty of Dentistry, Ataturk University, 25240 Erzurum, Turkiye. FAU - Kesmez, Ufuk AU - Kesmez U AUID- ORCID: 0009-0009-9016-1150 AD - Department of Periodontology, Faculty of Dentistry, Ataturk University, 25240 Erzurum, Turkiye. FAU - Aydin Tanas, Aysegul AU - Aydin Tanas A AUID- ORCID: 0009-0004-4996-0798 AD - Department of Periodontology, Faculty of Dentistry, Ataturk University, 25240 Erzurum, Turkiye. FAU - Simsek Derelioglu, Sera AU - Simsek Derelioglu S AUID- ORCID: 0000-0001-5192-923X AD - Department of Pediatric Dentistry, Faculty of Dentistry, Ataturk University, 25240 Erzurum, Turkiye. LA - eng PT - Journal Article PT - Review DEP - 20261002 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703020 OTO - NOTNLM OT - early childhood caries OT - frenulum attachment OT - maxillary anterior dental caries OT - maxillary labial frenulum OT - pediatric dentistry OT - scoping review COIS- S.S.D. is a co-author of one of the primary studies included in this scoping review, namely Yilgor et al. (2025) [20], To minimize the potential for bias, S.S.D. did not participate in the eligibility assessment, data extraction, or interpretation of that study. This relationship did not influence the eligibility criteria, study selection, synthesis, or conclusions of the review. The authors declare no financial conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/02 CRDT- 2026/10/27 10:37 PHST- 2026/09/13 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:37 [entrez] PHST- 2026/10/02 00:00 [pmc-release] AID - children13101340 [pii] AID - children-13-01340 [pii] AID - 10.3390/children13101340 [doi] PST - epublish SO - Children (Basel). 2026 Oct 2;13(10):1340. doi: 10.3390/children13101340. PMID- 42940649 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Sep 30 TI - Beyond Surgical Repair in Hypospadias: Genetics, Emerging Technologies, and Lifelong Outcomes-A Narrative Review. LID - 10.3390/children13101330 [doi] LID - 1330 AB - Background/Objectives: Hypospadias is a heterogeneous congenital anomaly. This selected narrative review examines emerging domains that may complement conventional surgical care, with particular emphasis on complex, proximal, atypical, familial, or disorder of sex development (DSD)-associated presentations and on long-term outcomes. Methods: A structured narrative search of PubMed/MEDLINE, Scopus, and Web of Science was conducted for English-language human or clinically applicable translational literature published from January 2000 through December 2025, supplemented by reference screening and targeted updating of genetics, non-operative management, and adult-outcome literature. Results: Objective phenotyping may improve anatomical description and research comparability. Endocrine/genetic evaluation should be considered in proximal hypospadias, including boys with descended testes, and is most clearly indicated when accompanied by cryptorchidism, micropenis, undervirilisation, familial disease, or other features suggestive of DSD; isolated distal hypospadias without such features does not generally require genetic or formal multidisciplinary DSD assessment. Limited evidence also supports explicit consideration of non-operative management in selected mild distal cases, while studies of uncorrected distal hypospadias report heterogeneous functional and cosmetic outcomes. Artificial intelligence (AI), quantitative tissue assessment, registries, and biobanks remain investigational. Long-term studies highlight sexual, reproductive, psychosocial, and late surgical outcomes that may extend into adulthood. Conclusions: The literature supports a selective, phenotype-driven framework rather than a universal multidisciplinary model, with particular attention to complex/atypical disease and lifelong outcome assessment. FAU - Alqadrie, Irfan Muhammad AU - Alqadrie IM AUID- ORCID: 0009-0007-3034-1112 AD - Department of Urology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta 10430, Indonesia. FAU - Raharja, Putu Angga Risky AU - Raharja PAR AUID- ORCID: 0000-0003-4951-2827 AD - Department of Urology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta 10430, Indonesia. FAU - Situmorang, Gerhard Reinaldi AU - Situmorang GR AD - Department of Urology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta 10430, Indonesia. FAU - Wahyudi, Irfan AU - Wahyudi I AD - Department of Urology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta 10430, Indonesia. FAU - Rodjani, Arry AU - Rodjani A AD - Department of Urology, Faculty of Medicine, Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta 10430, Indonesia. FAU - Abbas, Tariq AU - Abbas T AUID- ORCID: 0000-0002-8425-4029 AD - Pediatric Urology Section, Sidra Medicine, Doha P.O. Box 26999, Qatar. AD - College of Medicine, Qatar University, Doha P.O. Box 2713, Qatar. AD - Weill Cornell Medicine-Qatar, Doha P.O. Box 24144, Qatar. LA - eng PT - Journal Article PT - Review DEP - 20260930 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703442 OTO - NOTNLM OT - artificial intelligence OT - digital health OT - disorders of sex development OT - genetics OT - hypospadias OT - multidisciplinary care OT - pediatric urology OT - precision medicine OT - psychosocial support COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:36 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - children13101330 [pii] AID - children-13-01330 [pii] AID - 10.3390/children13101330 [doi] PST - epublish SO - Children (Basel). 2026 Sep 30;13(10):1330. doi: 10.3390/children13101330. PMID- 42940642 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Sep 29 TI - Assessment of the Occlusal Support Area in Children with Mixed Dentition: A Clinical Cross-Sectional Study from Oradea, Romania. LID - 10.3390/children13101323 [doi] LID - 1323 AB - Background/Objectives: The integrity of the primary teeth in the occlusal support area is essential for the eruption of the permanent successor teeth and for the development of a functional occlusion. This study aimed to clinically evaluate the health status of the primary teeth in the occlusal support area among children in the early mixed dentition attending an orthodontic practice in Oradea, Bihor County, Romania, and to analyze the pattern and prevalence of damage to this area by age and sex. Methods: This observational, cross-sectional, descriptive-analytical study was conducted at a specialized orthodontic practice in Oradea, Romania, between January and June 2026. A total of 255 patients aged 6-9 years (136 girls, 119 boys) were included, selected through consecutive sampling from an initial group of 308 patients. The evaluated variables included the integrity of the occlusal support area, the presence of caries, premature extractions, their combination, location, and the type of extracted primary molars. Comparisons between age and sex groups were performed using Fisher's Exact Test, with Bonferroni-corrected Z-tests. Results: The occlusal support area was damaged in 72.2% of patients, most frequently through a combination of premature extractions and caries (52.7%). Damage increased significantly with age, from 54.8% at 6 years to 80% at 8 years (p = 0.032). The second primary molar was the most frequently extracted tooth (62%). No significant sex-based differences were observed across the overall sample, except for a significant association at 7 years of age (p = 0.001), where girls more frequently presented isolated premature extractions. Conclusions: The occlusal support area is frequently affected in children with early mixed dentition who attended the orthodontic practice from Oradea, Romania, with damage increasing progressively between 6 and 8 years of age. These findings support the need for active monitoring and early preventive interventions during this critical developmental period. FAU - Cuc, Emilia Albinita AU - Cuc EA AD - Department of Dentistry, Faculty of Medicine and Pharmacy, University of Oradea, 4 Universitatii Street, 410087 Oradea, Romania. FAU - Moca, Rahela Tabita AU - Moca RT AD - Department of Dentistry, Faculty of Medicine and Pharmacy, University of Oradea, 4 Universitatii Street, 410087 Oradea, Romania. FAU - Berechet, Diana AU - Berechet D AUID- ORCID: 0000-0002-9649-197X AD - Department of Dentistry, Faculty of Medicine and Pharmacy, University of Oradea, 4 Universitatii Street, 410087 Oradea, Romania. FAU - Ciavoi, Gabriela AU - Ciavoi G AUID- ORCID: 0000-0003-1417-5963 AD - Department of Dentistry, Faculty of Medicine and Pharmacy, University of Oradea, 4 Universitatii Street, 410087 Oradea, Romania. FAU - Iurcov, Raluca AU - Iurcov R AUID- ORCID: 0000-0002-2953-3842 AD - Department of Dentistry, Faculty of Medicine and Pharmacy, University of Oradea, 4 Universitatii Street, 410087 Oradea, Romania. FAU - Tig, Ioan Andrei AU - Tig IA AUID- ORCID: 0000-0002-4897-9321 AD - Department of Dentistry, Faculty of Medicine and Pharmacy, University of Oradea, 4 Universitatii Street, 410087 Oradea, Romania. FAU - Benchea, Mihai AU - Benchea M AD - Department of Dentistry, Faculty of Medicine and Pharmacy, University of Oradea, 4 Universitatii Street, 410087 Oradea, Romania. FAU - Cuc, George Daniel AU - Cuc GD AD - Cluj-Napoca Emergency Clinical County Hospital, 3-5 Clinicilor Street, 400347 Cluj-Napoca, Romania. LA - eng PT - Journal Article DEP - 20260929 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13702945 OTO - NOTNLM OT - Romania OT - child OT - cross-sectional studies OT - deciduous OT - dental caries OT - dentition OT - mixed OT - tooth OT - tooth loss COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/29 CRDT- 2026/10/27 10:36 PHST- 2026/09/13 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/09/29 00:00 [pmc-release] AID - children13101323 [pii] AID - children-13-01323 [pii] AID - 10.3390/children13101323 [doi] PST - epublish SO - Children (Basel). 2026 Sep 29;13(10):1323. doi: 10.3390/children13101323. PMID- 42940634 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Sep 27 TI - The Role of the Family Microenvironment in Adolescents' Physical Activity and Nutritional Status. LID - 10.3390/children13101315 [doi] LID - 1315 AB - Background: Adolescents' physical activity, BMI-derived weight status, and weight perception are associated with their current and future health. This study examined the associations of family microenvironmental characteristics and perceived social support with these outcomes. Methods: A cross-sectional survey using convenience sampling was conducted among 411 Hungarian secondary school students aged 14-18 years. Physical activity was assessed using HBSC-based self-report items capturing weekly frequency and an operationalized indicator combining activity intensity and duration. Perceived social support was measured using the MSPSS. BMI-derived weight status was determined using age- and sex-specific percentiles calculated from self-reported height and weight. Chi-square tests and logistic regression models were applied. Results: Family background characteristics and perceived social support were not significantly associated with the examined physical activity indicators. Overall, 57.4% of participants had normal weight, while 26.7% were classified as having overweight or obesity. Only 55.2% accurately perceived their BMI-derived weight status. Exploratory coefficients suggested possible associations between selected family characteristics and BMI-derived weight status, although the corresponding overall models were not statistically significant. Higher perceived peer support was associated with lower odds of weight underestimation, whereas the association between perceived family support and weight overestimation did not reach statistical significance. Conclusions: Exploratory findings suggested possible associations of selected family and psychosocial characteristics with BMI-derived weight status and weight perception. Given the cross-sectional design, convenience sampling, self-reported anthropometric data, use of national BMI reference values, and limited explanatory performance of several models, the findings require cautious interpretation and should not be considered evidence of causal relationships. FAU - Karacsony, Ilona AU - Karacsony I AUID- ORCID: 0000-0003-3336-9376 AD - Institute of Basics of Health Sciences, Midwifery and Health Visiting, Faculty of Health Sciences, University of Pecs, 9700 Szombathely, Hungary. AD - Faculty of Health and Sport Sciences, Szechenyi Istvan University, 9026 Gyor, Hungary. FAU - Hideg-Feher, Gabriella AU - Hideg-Feher G AUID- ORCID: 0000-0002-2916-1145 AD - Institute of Physiotherapy and Sport Science, Faculty of Health Sciences, University of Pecs, 7622 Pecs, Hungary. LA - eng PT - Journal Article DEP - 20260927 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703084 OTO - NOTNLM OT - family microenvironment OT - nutritional status OT - overweight and obesity OT - perceived social support OT - physical activity OT - weight perception COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/27 CRDT- 2026/10/27 10:36 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - children13101315 [pii] AID - children-13-01315 [pii] AID - 10.3390/children13101315 [doi] PST - epublish SO - Children (Basel). 2026 Sep 27;13(10):1315. doi: 10.3390/children13101315. PMID- 42940633 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Sep 27 TI - Developmental Disparities and Associated Factors Among Children Aged 36-59 Months in Four Pakistani Provinces. LID - 10.3390/children13101314 [doi] LID - 1314 AB - BACKGROUND/OBJECTIVES: Early childhood developmental attainment varies across Pakistan, but provincial averages may obscure differences across developmental domains and socioeconomic groups. We examined developmental attainment and associated factors among children aged 36-59 months in four Pakistani provinces. METHODS: We analyzed four provincially representative UNICEF-supported Multiple Indicator Cluster Surveys (MICS) conducted between 2017 and 2020. Development was assessed using the original Early Childhood Development Index (ECDI). Survey-design-based analyses estimated overall and domain-specific prevalence, adjusted prevalence ratios (aPRs), and wealth-related inequalities. Province-specific associations were summarized using random-effects meta-analysis. RESULTS: Among 46,067 children, 59.4% met the overall ECDI criteria in Punjab, 54.6% in Khyber Pakhtunkhwa, 47.5% in Sindh, and 39.1% in Balochistan. This ordering persisted after age-sex standardization. Literacy-numeracy was the least frequently attained domain, ranging from 14.6% to 27.2%, whereas physical-domain attainment exceeded 90% in all four provinces. Early childhood education (ECE) attendance was associated with higher overall ECDI attainment (pooled aPR 1.29, 95% CI 1.22-1.37). Stunting and functional difficulty were associated with lower overall attainment, while associations with adult stimulation varied across provinces. Richest-poorest differences in overall ECDI prevalence ranged from 18.8 to 35.6 percentage points. CONCLUSIONS: Among children aged 36-59 months in four Pakistani provinces, developmental attainment differed substantially between provinces and across developmental domains, and these differences persisted after age-sex standardization. Socioeconomic inequalities were evident in every province. ECE attendance was associated with higher developmental attainment, but this association should be interpreted cautiously because the surveys were cross-sectional and may be affected by residual confounding and selection into ECE. FAU - Shah, Syed Sikandar AU - Shah SS AUID- ORCID: 0000-0003-4017-0664 AD - Department of Clinical Pharmacy and Pharmacology, RAK College of Pharmacy, RAK Medical and Health Sciences University, Ras Al Khaimah 11172, United Arab Emirates. FAU - Bukhari, Ashfaq Ahmad Shah AU - Bukhari AAS AD - Department of Physiology, RAK College of Medical Sciences, RAK Medical and Health Sciences University, Ras Al Khaimah 11172, United Arab Emirates. FAU - Ahmad, Jamil AU - Ahmad J AD - Department of Psychiatric Nursing, RAK College of Nursing, RAK Medical and Health Sciences University, Ras Al Khaimah 11172, United Arab Emirates. FAU - Alam, Aftab AU - Alam A AUID- ORCID: 0000-0002-6020-9290 AD - Department of Clinical Pharmacy and Pharmacology, RAK College of Pharmacy, RAK Medical and Health Sciences University, Ras Al Khaimah 11172, United Arab Emirates. FAU - Asghar, Junaid AU - Asghar J AD - Faculty of Pharmacy, Near East University, Near East Boulevard, Mersin 10, Nicosia 99138, Turkiye. LA - eng PT - Journal Article DEP - 20260927 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703014 OTO - NOTNLM OT - early childhood development index OT - early childhood education OT - multiple indicator cluster surveys OT - nurturing care OT - socioeconomic inequality OT - stunting COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/27 CRDT- 2026/10/27 10:36 PHST- 2026/08/19 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - children13101314 [pii] AID - children-13-01314 [pii] AID - 10.3390/children13101314 [doi] PST - epublish SO - Children (Basel). 2026 Sep 27;13(10):1314. doi: 10.3390/children13101314. PMID- 42940631 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Sep 26 TI - Guided Growth as a Component of Joint-Preserving Management of Complex Osteoarticular Pathology in the Growing Knee: A Pediatric Case Report. LID - 10.3390/children13101312 [doi] LID - 1312 AB - Background/Objectives: Valgus alignment shifts load toward the lateral compartment, and even a mild shift may matter when that compartment already contains a torn meniscus and an osteochondral lesion. We describe a skeletally immature knee in which a torn discoid lateral meniscus (DLM), an osteochondral lesion of the lateral femoral condyle and mild valgus coexisted. Case Presentation: A 14-year-old boy presented with longstanding right knee pain, lateral joint line tenderness and a positive McMurray test. The hip-knee-ankle (HKA) axis showed 4 degrees of valgus (contralateral 0.5 degrees ), with a mechanical lateral distal femoral angle (mLDFA) of 84.6 degrees , a medial proximal tibial angle (mMPTA) of 90.0 degrees and mechanical axis deviation (MAD) 10.78 mm lateral. Imaging and arthroscopy showed a torn, centrally shifted DLM and a 1.4 x 1.6 cm ICRS grade 4 lesion of the lateral femoral condyle. Saucerization with repair, microfracture, and temporary hemiepiphysiodesis of the medial distal femoral and proximal tibial physes were performed in one stage. Results: At five months, the HKA was 0.7 degrees varus (mLDFA, 87.2 degrees ; mMPTA, 88.3 degrees ) and the screws were removed; the repaired meniscus measured 9 mm in width without extrusion. At two years, the HKA was neutral (mLDFA, 87.0 degrees ; mMPTA, 87.7 degrees ; MAD, 0 mm) without rebound, and the patient was asymptomatic; no validated patient-reported outcome measure was collected. Conclusions: As a short-term result in a single patient, this case illustrates the feasibility of incorporating temporary hemiepiphysiodesis into a joint-preserving strategy in a selected skeletally immature knee. It is not evidence of efficacy; longer-term follow-up is needed to judge joint preservation. FAU - Kim, Kyeong Baek AU - Kim KB AUID- ORCID: 0000-0002-4308-8503 AD - Department of Orthopedic Surgery, Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, 20 Geumo-ro, Mulgeum-eup, Yangsan-si 50612, Gyeongsangnam-do, Republic of Korea. AD - Pusan National University, School of Medicine, Yangsan-si 50612, Gyeongsangnam-do, Republic of Korea. FAU - Kim, Jung Shin AU - Kim JS AUID- ORCID: 0000-0001-6155-5999 AD - Department of Orthopedic Surgery, College of Medicine, Dong-A University, Busan 49201, Republic of Korea. FAU - Cho, Jae Min AU - Cho JM AD - Department of Orthopedic Surgery, Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, 20 Geumo-ro, Mulgeum-eup, Yangsan-si 50612, Gyeongsangnam-do, Republic of Korea. AD - Pusan National University, School of Medicine, Yangsan-si 50612, Gyeongsangnam-do, Republic of Korea. FAU - Shin, Won Chul AU - Shin WC AD - Department of Orthopedic Surgery, Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, 20 Geumo-ro, Mulgeum-eup, Yangsan-si 50612, Gyeongsangnam-do, Republic of Korea. AD - Pusan National University, School of Medicine, Yangsan-si 50612, Gyeongsangnam-do, Republic of Korea. FAU - Do, Min Uk AU - Do MU AUID- ORCID: 0000-0002-0537-5376 AD - Department of Orthopedic Surgery, Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, 20 Geumo-ro, Mulgeum-eup, Yangsan-si 50612, Gyeongsangnam-do, Republic of Korea. AD - Pusan National University, School of Medicine, Yangsan-si 50612, Gyeongsangnam-do, Republic of Korea. FAU - Bae, Jung Yun AU - Bae JY AUID- ORCID: 0000-0001-8026-9330 AD - Department of Orthopedic Surgery, Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, 20 Geumo-ro, Mulgeum-eup, Yangsan-si 50612, Gyeongsangnam-do, Republic of Korea. AD - Pusan National University, School of Medicine, Yangsan-si 50612, Gyeongsangnam-do, Republic of Korea. FAU - Lee, Sang-Min AU - Lee SM AUID- ORCID: 0000-0001-7331-9539 AD - Department of Orthopedic Surgery, Research Institute for Convergence of Biomedical Science and Technology, Pusan National University Yangsan Hospital, 20 Geumo-ro, Mulgeum-eup, Yangsan-si 50612, Gyeongsangnam-do, Republic of Korea. AD - Pusan National University, School of Medicine, Yangsan-si 50612, Gyeongsangnam-do, Republic of Korea. AD - Shiley Center for Orthopaedic Research and Education at Scripps Clinic, Department of Molecular Medicine, Scripps Research, La Jolla, CA 92037, USA. LA - eng GR - 30-2026-008/Research Institute for Convergence of Biomedical Science and Technology, Pusan National Uni-versity Yangsan Hospital/ PT - Case Reports PT - Journal Article DEP - 20260926 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703123 OTO - NOTNLM OT - discoid lateral meniscus OT - genu valgum OT - guided growth OT - hemiepiphysiodesis OT - meniscal repair OT - osteochondral lesion OT - pediatric knee OT - saucerization COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/26 CRDT- 2026/10/27 10:36 PHST- 2026/08/27 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/09/26 00:00 [pmc-release] AID - children13101312 [pii] AID - children-13-01312 [pii] AID - 10.3390/children13101312 [doi] PST - epublish SO - Children (Basel). 2026 Sep 26;13(10):1312. doi: 10.3390/children13101312. PMID- 42940628 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Sep 26 TI - Learning Curve and Early Outcomes of Thoracoscopic Esophageal Atresia Repair in an Emerging Pediatric Minimally Invasive Surgery (MIS) Program: A Cumulative Sum Analysis. LID - 10.3390/children13101309 [doi] LID - 1309 AB - BACKGROUND: Thoracoscopic repair of esophageal atresia with tracheoesophageal fistula (EA/TEF) remains technically challenging due to the rarity of these procedures and the demanding intracorporeal suturing within the confined neonatal thorax. The aim of this study is to report the learning curve and early outcomes during the transition from open to thoracoscopic EA/TEF repair at a single center, evaluated using the cumulative sum (CUSUM) method. METHODS: We retrospectively reviewed data of 79 consecutive EA/TEF patients operated on between January 2015 and June 2026. Twenty-eight underwent thoracoscopic repair and 51 open repairs. The open cohort served as a historical institutional comparator, reflecting the standard of care prior to the adoption of thoracoscopic technique. The thoracoscopic group was stratified into three learning curve phases: Early (n = 9), Intermediate (n = 9), and Proficient (n = 10). Anastomotic leak was defined by contrast extravasation on postoperative esophagogram. Complications were graded using the Clavien-Dindo classification. RESULTS: The mean operative time was 2.9 +/- 1.2 h (thoracoscopic) vs. 2.3 +/- 1.2 h (open; p = 0.036). CUSUM analysis demonstrated a learning curve of approximately 15 cases, with operative time decreasing from 3.4 h (Phase 1) to 2.5 h (Phase 3; p = 0.034). Anastomotic leak occurred in 9/28 (32.1%) vs. 7/51 (13.7%; p = 0.137; underpowered comparison). Within the thoracoscopic group, long-gap patients (n = 8) had significantly higher stricture rates (37.5% vs. 0%; p = 0.017) and longer hospital stays (66 vs. 30 days; p = 0.005). The three long-gap patients with stricture required 11 total endoscopic dilation sessions. No statistically significant difference in mortality was observed (7.1% vs. 5.9%; p = 1.000). CONCLUSIONS: Thoracoscopic EA/TEF repair is feasible in an emerging MIS program with acceptable complication rates. The CUSUM-based learning curve was 15 cases. Long-gap thoracoscopic repair carries significantly higher stricture risk. FAU - Moga, Andreea AU - Moga A AD - Department of Pediatric Surgery, "Carol Davila" University of Medicine and Pharmacy, 050474 Bucharest, Romania. AD - Department of Pediatric Surgery, "Grigore Alexandrescu" Emergency Hospital for Children, 011743 Bucharest, Romania. FAU - Cimpeanu, Patricia AU - Cimpeanu P AD - Department of Pediatric Surgery, "Carol Davila" University of Medicine and Pharmacy, 050474 Bucharest, Romania. FAU - Balanescu, Laura AU - Balanescu L AUID- ORCID: 0000-0002-0815-0025 AD - Department of Pediatric Surgery, "Carol Davila" University of Medicine and Pharmacy, 050474 Bucharest, Romania. AD - Department of Pediatric Surgery, "Grigore Alexandrescu" Emergency Hospital for Children, 011743 Bucharest, Romania. FAU - Balanescu, Radu AU - Balanescu R AD - Department of Pediatric Surgery, "Carol Davila" University of Medicine and Pharmacy, 050474 Bucharest, Romania. AD - Department of Pediatric Surgery, "Grigore Alexandrescu" Emergency Hospital for Children, 011743 Bucharest, Romania. LA - eng PT - Journal Article DEP - 20260926 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703439 OTO - NOTNLM OT - CUSUM OT - anastomotic leak OT - esophageal atresia OT - learning curve OT - long-gap OT - minimally invasive surgery OT - thoracoscopy OT - tracheoesophageal fistula COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/26 CRDT- 2026/10/27 10:36 PHST- 2026/08/28 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/09/26 00:00 [pmc-release] AID - children13101309 [pii] AID - children-13-01309 [pii] AID - 10.3390/children13101309 [doi] PST - epublish SO - Children (Basel). 2026 Sep 26;13(10):1309. doi: 10.3390/children13101309. PMID- 42940626 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Sep 26 TI - Specific Factors Associated with Iatrogenic Withdrawal Syndrome in Children After Cardiac Surgery. LID - 10.3390/children13101307 [doi] LID - 1307 AB - Background/Objectives: Iatrogenic withdrawal syndrome (IWS) is common but underrecognized in critically ill children, and data from pediatric cardiac intensive care units (pCICUs) remain limited. This study determined the frequency of IWS after cardiac surgery and the factors associated with it. Methods: Retrospective, single-center cohort study of all pediatric patients admitted to a pCICU after cardiac surgery over 15 months. IWS screening was performed using the Withdrawal Assessment Tool-1 (WAT-1) at least three times daily post-extubation. IWS was defined as at least one WAT-1 score of 3 or higher. A prespecified multivariable logistic regression model with ten perioperative variables was fitted in the complete-case population. A subgroup analysis in ventilated children examined sedative and analgesic exposure, including the grouped duration of remifentanil infusion, in an adjusted model. Results: Of 311 patients, 294 (94.5%) were screened, and IWS occurred in 136 patients (46.3%, 95% CI 40.6-52.0). In the complete-case population (n = 286), univentricular physiology (OR 2.51, p = 0.044), suspected postoperative infection (OR 5.28, p = 0.023), and invasive ventilation for 10-100 h (OR 2.75, p = 0.021) or beyond 100 h (OR 5.29, p = 0.003) were independently associated with IWS, whereas postoperative lactatemia (>2 mmol/L for >6 h) was associated with lower odds (OR 0.39, p = 0.039). In mechanically ventilated children (n = 162), after adjustment, no sedative or analgesic exposure was significantly associated with IWS, while odds were substantially higher in patients receiving remifentanil beyond 48 h (OR 2.64, p = 0.114) and midazolam (OR 3.95, p = 0.057). Conclusions: In our institution, IWS was highly frequent after pediatric cardiac surgery, in particular in patients with univentricular physiology. In sedated patients, midazolam and prolonged remifentanil showed substantially higher, though not statistically significant, odds of developing IWS. These hypothesis-generating associations require prospective confirmation. FAU - Huners, Ida AU - Huners I AUID- ORCID: 0000-0002-4066-1116 AD - Department of Congenital and Pediatric Cardiac Surgery, Children's Heart Clinic, University Heart & Vascular Center, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany. FAU - Schumann, Sophia AU - Schumann S AD - Department of Pediatric Cardiology, Children's Heart Clinic, University Heart & Vascular Center, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany. FAU - Schon, Gerhard AU - Schon G AUID- ORCID: 0000-0003-2556-6804 AD - Institute of Medical Biometry and Epidemiology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany. FAU - Biermann, Daniel AU - Biermann D AUID- ORCID: 0000-0002-1895-8570 AD - Department of Congenital and Pediatric Cardiac Surgery, Children's Heart Clinic, University Heart & Vascular Center, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany. FAU - Denecke, Jonas AU - Denecke J AD - Section Pediatric Neurology, Department of Pediatrics, Center for Obstetrics and Pediatrics, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany. FAU - Gottschalk, Urda AU - Gottschalk U AD - Department of Pediatric Cardiology, Children's Heart Clinic, University Heart & Vascular Center, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany. FAU - Jurgensen, Peer P AU - Jurgensen PP AUID- ORCID: 0009-0002-0567-7929 AD - Department of Pediatric Cardiology, Children's Heart Clinic, University Heart & Vascular Center, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany. FAU - Siebel, Lena C AU - Siebel LC AD - Department of Pediatric Cardiology, Children's Heart Clinic, University Heart & Vascular Center, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany. FAU - Jess, Friederike AU - Jess F AD - Department of Pediatric Cardiology, Children's Heart Clinic, University Heart & Vascular Center, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany. FAU - Simons, Sinno H P AU - Simons SHP AUID- ORCID: 0000-0001-5219-5696 AD - Department of Pediatric and Neonatal Intensive Care, Division of Neonatology, Erasmus University Medical Center, 3015 GD Rotterdam, The Netherlands. FAU - Gleitze-Nolting, Carolin AU - Gleitze-Nolting C AD - Department of Pediatric Cardiology, Children's Heart Clinic, University Heart & Vascular Center, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany. FAU - Carstens, Henning AU - Carstens H AUID- ORCID: 0000-0002-9472-1663 AD - Department of Congenital and Pediatric Cardiac Surgery, Children's Heart Clinic, University Heart & Vascular Center, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany. FAU - Hubler, Michael AU - Hubler M AD - Department of Congenital and Pediatric Cardiac Surgery, Children's Heart Clinic, University Heart & Vascular Center, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany. FAU - Kozlik-Feldmann, Rainer G AU - Kozlik-Feldmann RG AUID- ORCID: 0000-0002-6222-2683 AD - Department of Pediatric Cardiology, Children's Heart Clinic, University Heart & Vascular Center, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany. FAU - Reiss, Irwin K M AU - Reiss IKM AD - Department of Pediatric and Neonatal Intensive Care, Division of Neonatology, Erasmus University Medical Center, 3015 GD Rotterdam, The Netherlands. AD - Section Neonatology and Pediatric Intensive Care Medicine, Department of Pediatrics, Center for Obstetrics and Pediatrics, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany. FAU - Harms, Sebastian H AU - Harms SH AUID- ORCID: 0000-0002-9034-344X AD - Department of Pediatric Cardiology, Children's Heart Clinic, University Heart & Vascular Center, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany. LA - eng PT - Journal Article DEP - 20260926 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703752 OTO - NOTNLM OT - cardiac surgery OT - iatrogenic withdrawal syndrome OT - pediatric cardiac intensive care OT - sedation weaning COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/26 CRDT- 2026/10/27 10:36 PHST- 2026/07/17 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/09/26 00:00 [pmc-release] AID - children13101307 [pii] AID - children-13-01307 [pii] AID - 10.3390/children13101307 [doi] PST - epublish SO - Children (Basel). 2026 Sep 26;13(10):1307. doi: 10.3390/children13101307. PMID- 42940621 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Sep 25 TI - From the First 1000 Days to the First 100 Years: Early-Life Biological Programming, Sentinel Phenotypes, and Healthy Aging. LID - 10.3390/children13101302 [doi] LID - 1302 AB - The first 1000 days of life, spanning from conception to approximately two years of age, represent a period of exceptional biological plasticity during which nutritional, metabolic, inflammatory, psychosocial, microbial, and environmental exposures may shape developmental trajectories across the life course. For this narrative review, PubMed/MEDLINE, Scopus, and Web of Science were searched from inception to June 2026, focusing primarily on human cohorts, systematic reviews, meta-analyses, guidelines, and consensus documents, with selected experimental studies included for mechanistic evidence. Within the framework of the Developmental Origins of Health and Disease (DOHaD), we examine the epigenetic, oxidative, inflammatory, mitochondrial, and telomere-related mechanisms proposed to link early-life exposures with lifelong health and biological aging. Because most human evidence in this field is observational, whereas much of the mechanistic evidence derives from experimental models, these relationships are presented as associations and biologically plausible hypotheses rather than as demonstrated causal pathways. We then consider how these processes may become clinically apparent during childhood through four illustrative "sentinel phenotypes"-allergic and atopic disease, respiratory vulnerability, renal-cardiovascular risk, and altered metabolic growth-proposed here as a conceptual framework rather than as an established classification. We also review preventive opportunities during pregnancy, infancy, and early childhood. Overall, the first 1000 days should be viewed as a critical window for developmental health, while the specific contribution of this review is to integrate early-life biological mechanisms with clinically observable pediatric sentinel phenotypes within the broader framework of biological and healthy aging. FAU - Pecoraro, Luca AU - Pecoraro L AUID- ORCID: 0000-0002-9765-8006 AD - Department of Experimental Medicine, Pediatric Section, University of Salento Hospital "Vito Fazzi", 73100 Lecce, Italy. FAU - Salaorni, Sebastiano AU - Salaorni S AUID- ORCID: 0009-0004-4366-7195 AD - Pediatric Division, Department of Surgical Sciences, Dentistry, Gynecology and Pediatrics, University of Verona, 37126 Verona, Italy. FAU - Mozzon, Sara AU - Mozzon S AD - Pediatric Division, Department of Surgical Sciences, Dentistry, Gynecology and Pediatrics, University of Verona, 37126 Verona, Italy. FAU - Russo, Riccardo AU - Russo R AD - Pediatric Division, Department of Surgical Sciences, Dentistry, Gynecology and Pediatrics, University of Verona, 37126 Verona, Italy. FAU - Renzetti, Gabriele AU - Renzetti G AD - Primary Care Pediatrician, Azienda ULSS 9 Scaligera, 37126 Verona, Italy. FAU - Indrio, Flavia AU - Indrio F AUID- ORCID: 0000-0001-9789-7878 AD - Department of Experimental Medicine, Pediatric Section, University of Salento Hospital "Vito Fazzi", 73100 Lecce, Italy. FAU - Piacentini, Giorgio AU - Piacentini G AD - Pediatric Division, Department of Surgical Sciences, Dentistry, Gynecology and Pediatrics, University of Verona, 37126 Verona, Italy. FAU - Pietrobelli, Angelo AU - Pietrobelli A AUID- ORCID: 0000-0002-9912-3232 AD - Pediatric Division, Department of Surgical Sciences, Dentistry, Gynecology and Pediatrics, University of Verona, 37126 Verona, Italy. AD - Pennington Biomedical Research Center, Baton Rouge, LA 70808, USA. LA - eng PT - Journal Article PT - Review DEP - 20260925 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703749 OTO - NOTNLM OT - biological aging OT - chronic inflammation OT - early-life programming OT - epigenetics OT - first 1000 days OT - healthy aging OT - life-course medicine OT - mitochondrial dysfunction OT - oxidative stress OT - telomere length COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/25 CRDT- 2026/10/27 10:36 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - children13101302 [pii] AID - children-13-01302 [pii] AID - 10.3390/children13101302 [doi] PST - epublish SO - Children (Basel). 2026 Sep 25;13(10):1302. doi: 10.3390/children13101302. PMID- 42940618 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Sep 24 TI - Sequential Prediction of Bronchopulmonary Dysplasia from Birth Through the First Week of Life Using Machine Learning. LID - 10.3390/children13101299 [doi] LID - 1299 AB - Background/Objectives: Bronchopulmonary dysplasia (BPD) risk evolves as clinical information accumulates after birth. This study evaluated the incremental predictive value of routinely available data from birth through the first week of life using cumulative, time-aligned feature sets. Methods: The primary analysis of this retrospective cohort study included 2153 preterm infants who survived to 36 weeks' postmenstrual age and had ascertainable BPD status and complete predictor data. The outcome was clinically documented BPD at 36 weeks' postmenstrual age, based on the treating neonatologists' assessment of continued supplemental oxygen and/or respiratory-support requirements. Three cumulative feature sets were constructed using variables available at or immediately after birth (Step 1), additional clinical and laboratory variables available within the first 24 h (Step 2), and further clinical-course variables available through postnatal day 7 (Step 3). Logistic regression, random forest, XGBoost, LightGBM, and CatBoost models were developed using a stratified 70:30 training-test split and five-fold stratified cross-validation. Internal validation assessed discrimination, calibration, and Brier scores in the held-out test set. AUROC confidence intervals and paired differences between prediction windows were estimated using 2000 bootstrap resamples. Predictor contributions were examined using SHapley Additive exPlanations. A multiple-imputation sensitivity analysis evaluated logistic regression and random forest in all 2616 infants with ascertainable BPD status. Results: Of the 2153 infants, 1154 (53.6%) had clinically documented BPD. Logistic regression achieved the highest held-out test-set AUROC at each prediction window: 0.751 (95% CI, 0.714-0.788) in Step 1, 0.897 (0.873-0.919) in Step 2, and 0.923 (0.902-0.942) in Step 3. Paired AUROC increases were 0.146 (95% CI, 0.110-0.181) from Step 1 to Step 2 and 0.026 (0.015-0.036) from Step 2 to Step 3. Logistic regression Brier scores decreased from 0.202 to 0.130 and 0.112, respectively. In the Step 3 logistic regression model, the leading predictors by mean absolute SHAP value were mean SpO(2) during the first 24 h, mean arterial pressure, initial feeding approach, FiO(2) at 24 h, and C-reactive protein. In the expanded sensitivity-analysis test cohort, logistic-regression AUROCs were 0.747, 0.893, and 0.913 for Steps 1-3, respectively. Conclusions: The addition of first-24 h clinical information substantially improved BPD prediction beyond birth variables, with further improvement from data accumulated through postnatal day 7. The primary analysis did not demonstrate an added predictive benefit of the evaluated tree-based algorithms over logistic regression. The primary findings apply to a selected cohort of survivors with complete data and should not be generalized to all initially eligible infants or interpreted as prediction of BPD or death. Temporal and external validation and prospective evaluation of clinical utility are required before implementation. FAU - Kim, Younghyun AU - Kim Y AUID- ORCID: 0000-0002-4287-4123 AD - Department of Pediatrics, Daegu Catholic University School of Medicine, Daegu 42472, Republic of Korea. FAU - Jeong, Ji Eun AU - Jeong JE AUID- ORCID: 0000-0002-3036-6818 AD - Department of Pediatrics, Daegu Catholic University School of Medicine, Daegu 42472, Republic of Korea. FAU - Kwak, Sang Gyu AU - Kwak SG AUID- ORCID: 0000-0003-0398-5514 AD - Department of Medical Statistics, Daegu Catholic University School of Medicine, Daegu 42472, Republic of Korea. LA - eng GR - RD-26-0033/Daegu Catholic University/ PT - Journal Article DEP - 20260924 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703402 OTO - NOTNLM OT - SHapley Additive exPlanations OT - bronchopulmonary dysplasia OT - machine learning OT - prediction model OT - preterm infants OT - sequential risk assessment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:36 PHST- 2026/08/12 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - children13101299 [pii] AID - children-13-01299 [pii] AID - 10.3390/children13101299 [doi] PST - epublish SO - Children (Basel). 2026 Sep 24;13(10):1299. doi: 10.3390/children13101299. PMID- 42940616 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Sep 24 TI - Health-Related Behaviors and BMI Across Three School Years: Annual Bayesian Network Patterns During the PETICA Childhood Obesity Prevention Program. LID - 10.3390/children13101298 [doi] LID - 1298 AB - Background/Objectives: This study examined annual probabilistic network structures among dietary habits, health-related attitudes, lifestyle behaviors, and body mass index (BMI) during implementation of the PETICA school-based program. Methods: This was a three-year prospective, uncontrolled school-based intervention with repeated measurements of the same cohort in Grades 2-4. The BMI analytic samples comprised 915 pupils in Grade 2 (461 boys, 454 girls; mean age 7.9 +/- 0.4), 840 in Grade 3 (429 boys, 411 girls; mean age 8.9 +/- 0.5), and 684 in Grade 4 (341 boys, 343 girls; mean age 9.9 +/- 0.4). BMI was recalculated from measured body mass and height and used as the continuous network outcome. BMI-for-age percentiles were retained for age- and sex-specific descriptive classification and sensitivity analysis. Separate score-based Bayesian networks were estimated for each grade. Network stability was assessed using 200 nonparametric bootstrap samples, with stable adjacency defined as a bootstrap frequency of at least 50%. Results: Fruit and vegetable intake formed a stable adjacency in all three grades, with bootstrap frequencies of 100.0% in Grade 2, 98.0% in Grade 3, and 99.0% in Grade 4. The sex of the pupil and computer/video-game use were stably connected in Grades 3 and 4 (100.0% in both grades). Stable connections among health-related attitudes were more prominent in later grades. In Grade 4, at-home breakfast consumption was connected with water intake (57.5%) and vegetable intake (52.0%). No behavioral adjacency with continuous BMI reached the prespecified 50% stability threshold in any grade. The largest subthreshold behavior-BMI signal was observed for at-home breakfast consumption in Grade 4 (39.0%). Conclusions: Selected relationships among reported health-related behaviors were reproducible across annual assessments, whereas direct behavior-BMI connections were year-specific and did not reach the stability threshold. The greater integration of attitudes and healthy habits in later grades is compatible with cumulative, age-adapted health education, but the absence of an untreated comparison group prevents attribution of these patterns to PETICA. The findings do not establish causal effects or program effectiveness, but may contribute to hypotheses generation for future evaluations of multi-component school-based obesityprevention programs. FAU - Cobal Pravdic, Sara AU - Cobal Pravdic S AUID- ORCID: 0000-0001-6956-6887 AD - Croatian Medical Association, Subiceva 9, 10000 Zagreb, Croatia. FAU - Vranesic Bender, Darija AU - Vranesic Bender D AD - Unit of Clinical Nutrition, Department of Internal Medicine, University Hospital Centre Zagreb, Kispaticeva 12, 10000 Zagreb, Croatia. FAU - Gajdos Kljusuric, Jasenka AU - Gajdos Kljusuric J AUID- ORCID: 0000-0001-6657-7337 AD - Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia. FAU - Kazazic, Ema AU - Kazazic E AD - School of Medicine, University of Zagreb, Salata 2, 10000 Zagreb, Croatia. FAU - Krznaric, Zeljko AU - Krznaric Z AD - Croatian Medical Association, Subiceva 9, 10000 Zagreb, Croatia. AD - Unit of Clinical Nutrition, Department of Internal Medicine, University Hospital Centre Zagreb, Kispaticeva 12, 10000 Zagreb, Croatia. AD - School of Medicine, University of Zagreb, Salata 2, 10000 Zagreb, Croatia. FAU - Kurtanjek, Zelimir AU - Kurtanjek Z AD - Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia. LA - eng GR - Croatian Medical Association/ PT - Journal Article DEP - 20260924 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703612 OTO - NOTNLM OT - BMI OT - BMI-for-age percentiles OT - Bayesian networks OT - dietary habits OT - lifestyle behaviors OT - prospective study OT - public-health program "PETICA" COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:36 PHST- 2026/08/03 00:00 [received] PHST- 2026/09/11 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - children13101298 [pii] AID - children-13-01298 [pii] AID - 10.3390/children13101298 [doi] PST - epublish SO - Children (Basel). 2026 Sep 24;13(10):1298. doi: 10.3390/children13101298. PMID- 42940613 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Sep 24 TI - Procedural Sedation in Pediatric Gastroenterology: A Narrative Review from the Shared Perspective of Pediatric Gastroenterology and Anesthesiology. LID - 10.3390/children13101294 [doi] LID - 1294 AB - Background/Objectives: Endoscopic and interventional procedures in pediatric gastroenterology are increasing in number and complexity, and most require sedation or, in selected cases, general anesthesia (GA); children face a higher risk of serious sedation-related adverse events than adults. Written jointly by pediatric gastroenterologists and anesthesiologists, this review provides a practical, evidence-referenced framework for procedural sedation across pediatric gastrointestinal procedures. Methods: We performed a narrative review of PubMed/MEDLINE (inception to 20 June 2026, the date of the final search), prioritizing society guidelines, systematic reviews, randomized controlled trials, and large registries on pediatric procedural sedation, sedative agents, capnography, and high-flow nasal oxygen (HFNO). Results: Sedation should be matched to the procedure and to a structured risk tier, targeting the lightest depth that allows safe completion. Propofol-, ketamine-, and dexmedetomidine-based regimens dominate; remimazolam and esketamine are emerging, but pediatric data are limited. Capnography detects hypoventilation before desaturation, and HFNO reduces hypoxemia in adults, whereas pediatric-specific evidence remains limited and the pediatric subgroup analysis did not reach statistical significance. Higher-risk groups require tailored planning, monitoring, and rescue readiness; most evidence is observational or extrapolated from adults. Conclusions: Safe pediatric gastrointestinal sedation combines structured risk assessment, rational drug combinations, capnography-supported monitoring, and consistent complication reporting. Who administers deep sedation, and whether general anesthesia is preferred, should depend on patient and procedural risk together with local regulations, institutional policy, training and credentialing, and rescue capability, within a shared gastroenterology-anesthesiology framework. Adequately powered pediatric trials of HFNO and of remimazolam/esketamine, and long-term neurodevelopmental follow-up, are priorities. FAU - Cesur, Sevim AU - Cesur S AUID- ORCID: 0000-0002-8764-1251 AD - Department of Anesthesiology and Reanimation, Faculty of Medicine, Kocaeli University, 41001 Kocaeli, Turkiye. FAU - Uyar Aksu, Nihal AU - Uyar Aksu N AUID- ORCID: 0000-0002-0270-2589 AD - Department of Pediatric Gastroenterology, Hepatology and Nutrition, Faculty of Medicine, Kocaeli University, 41001 Kocaeli, Turkiye. FAU - Uncuoglu, Aysen AU - Uncuoglu A AD - Department of Pediatric Gastroenterology, Hepatology and Nutrition, Faculty of Medicine, Kocaeli University, 41001 Kocaeli, Turkiye. FAU - Cardakozu, Tulay AU - Cardakozu T AD - Department of Anesthesiology and Reanimation, Faculty of Medicine, Kocaeli University, 41001 Kocaeli, Turkiye. FAU - Icli, Dilek AU - Icli D AD - Department of Anesthesiology and Reanimation, Faculty of Medicine, Kocaeli University, 41001 Kocaeli, Turkiye. LA - eng PT - Journal Article PT - Review DEP - 20260924 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703528 OTO - NOTNLM OT - capnography OT - dexmedetomidine OT - gastrointestinal endoscopy OT - high-flow nasal oxygen OT - ketamine OT - pediatric sedation OT - propofol OT - remimazolam OT - risk stratification COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:36 PHST- 2026/08/05 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - children13101294 [pii] AID - children-13-01294 [pii] AID - 10.3390/children13101294 [doi] PST - epublish SO - Children (Basel). 2026 Sep 24;13(10):1294. doi: 10.3390/children13101294. PMID- 42940612 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9067 (Print) IS - 2227-9067 (Electronic) IS - 2227-9067 (Linking) VI - 13 IP - 10 DP - 2026 Sep 23 TI - Development of a Nomogram Model for Predicting Mortality Risk in Critically Ill Children with Influenza. LID - 10.3390/children13101293 [doi] LID - 1293 AB - BACKGROUND: Influenza virus commonly infects children. Children with critical infection may develop influenza-associated encephalopathy or encephalitis, conditions rarely seen in adults and associated with high mortality. This results in growing healthcare resource consumption and family burden. OBJECTIVE: This study aimed to identify the predictors for death in critically ill children with influenza, so as to facilitate early recognition of high-risk patients, guide clinical interventions, and reduce mortality. METHODS: We retrospectively analyzed the clinical data of critically ill children with influenza admitted to the Children's Hospital of Soochow University between January 2018 and January 2026. L1-penalized binomial logistic regression (LASSO) was used for variable selection. Variables selected by LASSO were entered into a Firth penalized logistic regression model to identify independent predictors of mortality, based on which a nomogram was constructed to estimate mortality risk. Model discrimination was assessed using the receiver operating characteristic (ROC) curve and area under the curve (AUC). Bootstrap resampling was performed for internal validation and optimism correction. Model calibration was assessed using calibration curves, and decision curve analysis (DCA) was performed to evaluate the clinical net benefit of the model. RESULTS: A total of 113 critically ill children with influenza were included, of whom 34 died, corresponding to a mortality rate of 30.1%. LASSO regression was used for variable selection, followed by Firth penalized logistic regression to develop a mortality risk prediction model. The final model included five variables: Hgb, PLT, UREA, ALB, and confusion. A mortality risk nomogram was developed based on these five predictors and internally validated using bootstrap resampling. The ROC curve showed good discrimination of the nomogram in internal validation. The calibration curve demonstrated good agreement between the predicted probabilities and observed outcomes. DCA showed that the nomogram provided a higher net benefit across a certain range of threshold probabilities, indicating its potential clinical utility. CONCLUSIONS: Hgb, PLT, UREA, ALB, and confusion are important predictors of mortality in critically ill children with influenza. The nomogram developed based on these predictors showed good discrimination and calibration, as well as potential clinical net benefit, and may serve as an adjunctive tool for early identification of mortality risk and clinical decision-making in critically ill children with influenza. FAU - Ye, Yingying AU - Ye Y AD - Department of Infectious Diseases, Children's Hospital of Soochow University, No. 303, Jingde Road, Suzhou 215003, China. FAU - Zhang, Zhiling AU - Zhang Z AD - Department of Infectious Diseases, Children's Hospital of Soochow University, No. 303, Jingde Road, Suzhou 215003, China. FAU - Chen, Jianlong AU - Chen J AD - Department of Infectious Diseases, Children's Hospital of Soochow University, No. 303, Jingde Road, Suzhou 215003, China. FAU - Sun, Huiming AU - Sun H AD - Department of Respiratory Medicine, Children's Hospital of Soochow University, No. 303, Jingde Road, Suzhou 215003, China. FAU - Bai, Zhenjiang AU - Bai Z AD - Department of Pediatric Intensive Care Unit, Children's Hospital of Soochow University, No. 92, Zhongnan Road, Suzhou 215025, China. FAU - Zhou, Weifang AU - Zhou W AUID- ORCID: 0000-0001-6088-4977 AD - Department of Infectious Diseases, Children's Hospital of Soochow University, No. 303, Jingde Road, Suzhou 215003, China. FAU - Jiang, Yanqun AU - Jiang Y AD - Department of Infectious Diseases, Children's Hospital of Soochow University, No. 303, Jingde Road, Suzhou 215003, China. LA - eng GR - GSWS2023047/Suzhou Municipal Government/ PT - Journal Article DEP - 20260923 PL - Switzerland TA - Children (Basel) JT - Children (Basel, Switzerland) JID - 101648936 PMC - PMC13703509 OTO - NOTNLM OT - critically ill children OT - influenza OT - mortality OT - nomogram OT - predictors COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/23 CRDT- 2026/10/27 10:36 PHST- 2026/07/15 00:00 [received] PHST- 2026/09/03 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - children13101293 [pii] AID - children-13-01293 [pii] AID - 10.3390/children13101293 [doi] PST - epublish SO - Children (Basel). 2026 Sep 23;13(10):1293. doi: 10.3390/children13101293. PMID- 42940600 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 21 TI - Quantitative Workload and Work Engagement Among Community Pharmacy Technicians: The Moderating Role of Perceived Supervisor Support. LID - 10.3390/healthcare14203555 [doi] LID - 3555 AB - Background/Objectives: Research on community pharmacy technicians has rarely examined how workload and supervisor support jointly relate to work engagement. The aim of this study was to examine these relationships among community pharmacy technicians within the Job Demands-Resources framework and to test the moderating role of supervisor support in the workload-work engagement relationship. Methods: This cross-sectional study included 574 community pharmacy technicians recruited from pharmacies selected through stratified random sampling in four provinces of Turkiye. Quantitative workload, perceived supervisor support, and work engagement were measured using validated instruments. Moderation was tested with PROCESS Model 1. Results: When supervisor support was at its mean, higher quantitative workload was associated with lower work engagement (B = -0.290, 95% CI [-0.389, -0.191], p < 0.001). At the mean workload level, supervisor support was positively associated with engagement (B = 0.405, 95% CI [0.295, 0.516], p < 0.001). The interaction between workload and supervisor support was significant but modest in magnitude (B = 0.156, 95% CI [0.041, 0.271], p = 0.008; DeltaR(2) = 0.010). Simple slopes showed a progressively weaker negative workload-engagement association as supervisor support increased. The interaction remained significant in the covariate-adjusted model (B = 0.148, 95% CI [0.030, 0.266], p = 0.014). Conclusions: Higher quantitative workload was associated with lower work engagement, whereas greater perceived supervisor support was associated with higher engagement. The negative workload-engagement association also weakened as perceived supervisor support increased. FAU - Ozkaya, Yagmur AU - Ozkaya Y AUID- ORCID: 0000-0003-2937-0366 AD - Department of Pharmacy Management, Faculty of Pharmacy, Erzincan Binali Yildirim University, Erzincan 24002, Turkiye. FAU - Hakverdi, Gulden AU - Hakverdi G AUID- ORCID: 0000-0002-8078-0451 AD - Department of Biostatistics, Faculty of Medicine, Sivas Cumhuriyet University, Sivas 58140, Turkiye. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704525 OTO - NOTNLM OT - Job Demands-Resources theory OT - community pharmacy OT - perceived supervisor support OT - pharmacy technicians OT - pharmacy workforce OT - quantitative workload OT - work engagement COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:36 PHST- 2026/09/06 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - healthcare14203555 [pii] AID - healthcare-14-03555 [pii] AID - 10.3390/healthcare14203555 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 21;14(20):3555. doi: 10.3390/healthcare14203555. PMID- 42940599 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 21 TI - Stakeholder-Reported Requirements for Future Artificial Intelligence Decision Support in Periodontal Care: A Cross-Sectional Survey of Dentists, Physicians and Patients in Kazakhstan. LID - 10.3390/healthcare14203554 [doi] LID - 3554 AB - Background/Objectives: Clinicians' expectations of artificial intelligence (AI) for periodontal care in Central Asia are largely unknown. We surveyed dentists and physicians in Kazakhstan on preliminary requirements for a future decision-support system and explored patients' self-reported periodontal signs. Methods: Cross-sectional online surveys (July-September 2026; non-probability recruitment) included 33 dentists, 91 primary health care physicians and medical specialists, and 455 adult patients whose periodontal eligibility was not clinically verified; the patient questionnaire did not address AI. Analyses used Wilson 95% confidence intervals (CI), exact tests with Holm adjustment and exploratory logistic regression. Results: Dentists prioritised history-taking (81.8%) and early gingivitis diagnosis (69.7%). Autonomy choices were spread across five clinician-controlled configurations (low autonomy most frequent, 30.3%); no autonomous option was offered. Lack of a legal framework and liability insurance was the most cited barrier (60.6%). Physicians and specialists selected early gingivitis screening (80.2%) but cited insufficient competence (53.8%). Self-reported signs occurred in 49.0% of patients. Associations with high stress (adjusted odds ratio 2.11; 95% CI 1.14-3.89) and family history (1.61; 1.02-2.55) lost significance after excluding 204 responses collected via an inter-university platform. Conclusions: Clinician responses suggest preliminary requirements; patient associations remain exploratory. Instrument and clinical outcome validation should precede system design. FAU - Ayash, Yerbol AU - Ayash Y AUID- ORCID: 0009-0009-3552-6233 AD - Center of Epidemiology and Management, Astana Medical University, Astana010000 , Kazakhstan. FAU - Ismailova, Aigul AU - Ismailova A AD - Center of Epidemiology and Management, Astana Medical University, Astana010000 , Kazakhstan. FAU - Aidarkhanova, Anar AU - Aidarkhanova A AD - Center of Epidemiology and Management, Astana Medical University, Astana010000 , Kazakhstan. FAU - Mussina, Aiman AU - Mussina A AD - Center of Epidemiology and Management, Astana Medical University, Astana010000 , Kazakhstan. FAU - Abdikadyr, Zhanat AU - Abdikadyr Z AD - Center of Epidemiology and Management, Astana Medical University, Astana010000 , Kazakhstan. FAU - Chayakova, Akerke AU - Chayakova A AUID- ORCID: 0000-0002-8398-3684 AD - Center of Epidemiology and Management, Astana Medical University, Astana010000 , Kazakhstan. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704399 OTO - NOTNLM OT - Kazakhstan OT - artificial intelligence OT - clinical decision support systems OT - human-AI collaboration OT - medical liability OT - periodontal diseases OT - periodontal screening OT - primary health care OT - stakeholder survey OT - survey research COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:36 PHST- 2026/09/30 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - healthcare14203554 [pii] AID - healthcare-14-03554 [pii] AID - 10.3390/healthcare14203554 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 21;14(20):3554. doi: 10.3390/healthcare14203554. PMID- 42940596 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 21 TI - Decision-Architecture Dependence and Patient-Gender-Conditioned Allocation in Large-Language-Model-Assisted Nursing Allocation: A Multi-Model Counterfactual Audit. LID - 10.3390/healthcare14203549 [doi] LID - 3549 AB - Background/Objectives: Large language models (LLMs) may support healthcare workforce allocation, but their decisions can be sensitive to demographic cues and decision architecture. This study examined gender-task congruity, Candidate-A dependence under the tested decision architecture, and patient-gender-conditioned nursing allocation. Methods: Three provider-specific LLM configurations were audited across 24 nursing tasks. Male and female nurses were professionally equivalent and counterbalanced across Candidate A/B; patient gender was varied in six intimate-care tasks. Sixty conditions were repeated three times per model, yielding 540 valid outputs. Results: Male nurses were selected in 50.0% of Technical/Procedural and Relational/Educational allocations. Candidate A was selected in all 288 observations, although 285 (99.0%) assigned identical suitability scores. Because A/B assignment was bundled with labeling, presentation order, evaluation order, and response-template anchoring, this pattern indicates Candidate-A dependence under the tested decision architecture rather than an isolated position effect. Changing patient gender from female to male produced 57 female-to-male nurse switches and no reverse switches (p < 0.001). Conclusions: The audited configurations showed no generalized gender-task pattern but demonstrated decision-architecture dependence and patient-gender-conditioned allocation. Fairness audits should assess equivalent-candidate handling, patient preference, and counterfactual stability. FAU - Li, Zhiying AU - Li Z AD - Guangxi Hospital Division of the First Affiliated Hospital, Sun Yat-sen University, Nanning 530022, China. FAU - Zhu, Wenjie AU - Zhu W AUID- ORCID: 0009-0002-6750-4038 AD - Faculty of Humanities, Management and Science, Universiti Putra Malaysia Sarawak, Bintulu 97008, Malaysia. FAU - Jiang, Luodi AU - Jiang L AD - Faculty of Business Administration, University of Macau, Avenida da Universidade, Taipa, Macau 999078, China. FAU - Wei, Xiuzhen AU - Wei X AD - Guangxi Hospital Division of the First Affiliated Hospital, Sun Yat-sen University, Nanning 530022, China. FAU - Zhou, Jie AU - Zhou J AD - Guangxi Hospital Division of the First Affiliated Hospital, Sun Yat-sen University, Nanning 530022, China. FAU - Yang, Wei AU - Yang W AD - The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510000, China. FAU - Zhong, Baoling AU - Zhong B AD - The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510000, China. FAU - Hu, Qiongyan AU - Hu Q AD - The Peoples Hospital of Guangxi Zhuang Autonomous Region, Nanning 530022, China. LA - eng GR - GuiKe AB24010147/Guangxi Science and Technology Department/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704475 OTO - NOTNLM OT - Candidate-A dependence OT - Internet Plus Nursing Service OT - algorithmic fairness OT - counterfactual audit OT - decision architecture OT - gender-conditioned allocation OT - large language models OT - nursing workforce allocation COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analysis, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:36 PHST- 2026/08/17 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - healthcare14203549 [pii] AID - healthcare-14-03549 [pii] AID - 10.3390/healthcare14203549 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 21;14(20):3549. doi: 10.3390/healthcare14203549. PMID- 42940595 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 21 TI - Factors Associated with Transplant-Specific Psychological Adaptation Among Heart and Kidney Transplant Recipients. LID - 10.3390/healthcare14203551 [doi] LID - 3551 AB - BACKGROUND: Psychological adaptation after solid organ transplantation extends beyond graft survival and includes transplant-specific emotional responses that may influence long-term health outcomes. However, factors associated with transplant-specific psychological adaptation remain insufficiently understood, particularly in Central European populations. METHODS: A multicentre cross-sectional study was conducted among 224 adult heart (n = 107) and kidney (n = 117) transplant recipients attending three Polish transplant centers. Participants completed the Transplant Effects Questionnaire (TxEQ), Hospital Anxiety and Depression Scale (HADS), General Self-Efficacy Scale (GSES), Life Orientation Test-Revised (LOT-R), and SF-36. We performed univariable and multivariable linear regression analyses separately for heart and kidney transplant recipients to identify factors associated with transplant-specific psychological adaptation. RESULTS: Overall psychological adaptation was favorable. Adherence demonstrated the highest TxEQ scores, whereas donor-related guilt and disclosure concerns were relatively low. Approximately 20.53% of participants reported clinically relevant anxiety symptoms and 14.2% reported depressive symptoms. In heart transplant recipients, better mental health-related quality of life was independently associated with greater adherence (beta = 0.025, p = 0.003). Among kidney transplant recipients, higher donor-related guilt was associated with transplantation center, while greater disclosure was associated with male sex, transplantation center, and shorter time since transplantation (all p < 0.05). Several psychological variables were associated with selected domains of transplant-specific psychological adaptation, whereas demographic and clinical variables showed fewer significant associations. CONCLUSIONS: These findings indicate that several psychological variables were associated with transplant-specific psychological adaptation in long-term heart and kidney transplant recipients. Routine assessment of anxiety, mental health-related quality of life, and transplant-specific emotional responses may facilitate identification of recipients who could benefit from further psychosocial evaluation. Prospective studies are needed to determine whether these associations translate into improved long-term outcomes. FAU - Milaniak, Irena AU - Milaniak I AUID- ORCID: 0000-0002-6649-7545 AD - Faculty of Health Sciences, Andrzej Frycz Modrzewski Krakow University, 30-705 Krakow, Poland. FAU - Pawlikowska, Roksana AU - Pawlikowska R AD - Faculty of Health Sciences, Andrzej Frycz Modrzewski Krakow University, 30-705 Krakow, Poland. FAU - Debska, Grazyna AU - Debska G AD - Medical Institute, University of Applied Sciences in Nowy Targ, 34-400 Nowy Targ, Poland. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704155 OTO - NOTNLM OT - adaptation OT - psychosocial assessment OT - transplantation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:36 PHST- 2026/08/26 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - healthcare14203551 [pii] AID - healthcare-14-03551 [pii] AID - 10.3390/healthcare14203551 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 21;14(20):3551. doi: 10.3390/healthcare14203551. PMID- 42940579 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 20 TI - Addressing Care Gaps in Chronic Disease Management: A Systematic Review of Nursing Interventions. LID - 10.3390/healthcare14203534 [doi] LID - 3534 AB - Background/Objectives: Chronic diseases are a major contributor to the global burden of disease and are often associated with care gaps that adversely affect health outcomes and healthcare quality. Nurses play a central role in delivering interventions to address these gaps. This systematic review aimed to identify which nursing interventions address care gaps in chronic disease management and to assess the outcomes of their implementation among adults with chronic diseases. Methods: A systematic review was conducted in accordance with PRISMA guidelines. Five electronic databases were searched for studies. Studies involving adults with chronic diseases that evaluated nursing interventions targeting care gaps were eligible for inclusion. Methodological quality was assessed using JBI critical appraisal tools appropriate for the study design, and studies were not excluded based on quality assessment. Data from primary studies and systematic reviews were synthesized narratively and separately to avoid double counting of evidence. Overlap between included systematic reviews and primary studies was assessed and documented. Results: Twenty reports were included and synthesized separately: 17 reports of 16 primary studies (randomized and cluster-randomized controlled trials, quasi-experimental studies, and one cohort study) and three systematic reviews with meta-analysis. In the primary studies, the most frequently targeted care gap was continuity of care, followed by unmet needs, fragmented care, and health literacy or information gaps. The most common interventions were nurse-led case management, telehealth follow-up, and self-management support. Reported improvements, mostly in outcomes related to rather than direct measures of the targeted gaps, included care coordination, self-management behaviors, treatment adherence, quality of life, patient satisfaction, and selected clinical outcomes. Of the 17 primary-study reports, eight showed a statistically significant between-group benefit on the primary outcome, and four showed mixed effects; the remainder showed non-significant, within-group-only, or uncontrolled changes or did not test effectiveness. The three systematic reviews of transitional care and nurse-managed protocols reported reductions in readmissions and mortality and modest improvements in clinical risk factors. Conclusions: Nurse-led interventions are frequently designed to target care gaps in chronic disease management and have been associated with improvements in selected patient, service, and clinical outcomes. However, few studies measured the targeted gaps directly, so evidence that these gaps actually narrowed remains limited. Case management, telehealth follow-up, and self-management support were the most frequently evaluated approaches and warrant rigorous evaluation using direct measures of care gaps. FAU - Cussigh, Barbara AU - Cussigh B AD - Faculty of Health Sciences, University of Maribor, 2000 Maribor, Slovenia. FAU - Gosak, Lucija AU - Gosak L AUID- ORCID: 0000-0002-8742-6594 AD - Faculty of Health Sciences, University of Maribor, 2000 Maribor, Slovenia. FAU - Trajbaric, Tamara AU - Trajbaric T AUID- ORCID: 0009-0002-2898-5924 AD - Faculty of Health Sciences, University of Maribor, 2000 Maribor, Slovenia. FAU - Svensek, Adrijana AU - Svensek A AUID- ORCID: 0000-0003-0323-1720 AD - Faculty of Health Sciences, University of Maribor, 2000 Maribor, Slovenia. LA - eng PT - Journal Article PT - Review DEP - 20261020 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704512 OTO - NOTNLM OT - care gaps OT - case management OT - chronic disease management OT - continuity of care OT - multimorbidity OT - nursing interventions OT - self-management support OT - telehealth COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:36 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - healthcare14203534 [pii] AID - healthcare-14-03534 [pii] AID - 10.3390/healthcare14203534 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 20;14(20):3534. doi: 10.3390/healthcare14203534. PMID- 42940578 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 20 TI - Global Research Trends in Treatments for Ureteropelvic Junction Obstruction: A Multi-Database Bibliometric Analysis of the Past Two Decades. LID - 10.3390/healthcare14203533 [doi] LID - 3533 AB - Background: Ureteropelvic junction obstruction (UPJO) is one of the most common causes of hydronephrosis in children. Over the last several years, treatment methods have evolved toward less invasive procedures. However, bibliometric analysis of UPJO treatment remains limited. We applied a multi-database bibliometric approach to describe the current status and recent and emerging research trends in UPJO management. Methods: We retrieved studies on the treatment of UPJO from PubMed, Scopus, Embase, Web of Science, and Cochrane Library, spanning from 1 January 2006 to 31 December 2025; all five databases were searched on 20 January 2026. Records were merged in EndNote X9 (Clarivate Analytics, Philadelphia, PA, USA), deduplicated using Author, Year, and Title, and manually verified using DOI and minor title/author variants. Web of Science was used as the single source for citation counts and cited-reference data in citation-based analyses. Of the 849 included publications, 736 were matched to Web of Science, and 728 of these contributed cited-reference data to the co-citation analysis. We analyzed data using CiteSpace (v6.2.R.3; one-year slices; g-index k = 25) together with the bibliometrix R package (v4.6). Results: A total of 849 publications, including 761 original articles and 88 reviews, from 55 countries were included in the analysis, with the United States and China leading in publication volume. Annual citation activity generally increased with year-to-year variation. A total of 3545 authors participated in these publications, among whom Lorenzo AJ, Noh PH, and Peters CA had the highest total citation counts. Wroclaw Medical University, Peking University and University of Toronto had the highest publication counts among institutions. The funding analysis indicated that the National Natural Science Foundation of China, United States Department of Health and Human Services, and National Institutes of Health were the top three funding bodies by publication count. Conclusions: Management-related research on UPJO is active, with current emphasis on technological innovation, full-cycle management, and complex case management. Robot-assisted pyeloplasty remains a prominent bibliometric theme in the recent literature. FAU - Xiang, Lei AU - Xiang L AD - Department of Urology, Institute of Urology (Laboratory of Reconstructive Urology), West China Hospital, Sichuan University, Chengdu 610041, China. FAU - He, Qiyu AU - He Q AUID- ORCID: 0000-0002-5414-0207 AD - Department of Urology, Institute of Urology (Laboratory of Reconstructive Urology), West China Hospital, Sichuan University, Chengdu 610041, China. FAU - Lin, Lede AU - Lin L AD - Department of Urology, Institute of Urology (Laboratory of Reconstructive Urology), West China Hospital, Sichuan University, Chengdu 610041, China. FAU - Zhou, Liang AU - Zhou L AUID- ORCID: 0000-0003-3439-0222 AD - Department of Urology, Institute of Urology (Laboratory of Reconstructive Urology), West China Hospital, Sichuan University, Chengdu 610041, China. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704246 OTO - NOTNLM OT - bibliometric analysis OT - surgical plan OT - treatment OT - ureteropelvic junction obstruction COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:36 PHST- 2026/08/14 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - healthcare14203533 [pii] AID - healthcare-14-03533 [pii] AID - 10.3390/healthcare14203533 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 20;14(20):3533. doi: 10.3390/healthcare14203533. PMID- 42940576 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 20 TI - Beyond Individual Fault: A Context-Sensitive Framework for Assessing Medical Liability in Obstetrics and Gynaecology. LID - 10.3390/healthcare14203531 [doi] LID - 3531 AB - BACKGROUND/OBJECTIVES: Previous obstetric and gynaecological malpractice reviews identify recurring concerns but do not specify a sequence for examining conduct before deviation, causation and attribution. We developed a context-sensitive conceptual framework. METHODS: A structured critical narrative review of PubMed/MEDLINE, Scopus and Web of Science Core Collection was combined with analysis of Romanian legal and professional sources. The broad searches yielded 1785 database records before deduplication. The team's legal specialist manually screened the overlapping thematic searches 2A-2D using her subject expertise; duplicates were removed and 223 unique publications were retained. The 347 records reported for thematic search 2A alone are not the input denominator for that corpus. She extracted the information for 40 selected anchor publications, mapping them to eight broad themes. The thirteen-domain Context-Sensitive Liability Assessment Framework for Obstetrics and Gynaecology (CSLAF-OG) was illustrated using three principal Romanian judicial cases and one additional case. RESULTS: The framework orders context, professional standards, autonomy, communication, documentation and organisational conditions before harm, deviation, causation and attribution. CONCLUSIONS: CSLAF-OG is a conceptual aid for retrospective analysis, not a validated instrument or legal test. Independent evaluation remains necessary. FAU - Patru, Larisa AU - Patru L AUID- ORCID: 0000-0002-6897-3381 AD - Department 9, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Comanescu, Maria Cristina AU - Comanescu MC AD - Department of Anatomy, Faculty of Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Andrei, Elena Cristina AU - Andrei EC AUID- ORCID: 0009-0008-1581-8928 AD - Department of Histology, Faculty of Dentistry, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. AD - Research Centre for Microscopic Morphology and Immunology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. AD - Dental Medicine Research Center, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Micu, Daniela-Maria AU - Micu DM AD - Department of Foreign Languages, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Capitanescu, Razvan Grigoras AU - Capitanescu RG AUID- ORCID: 0009-0005-8535-6640 AD - Department 8 Mother and Child, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Patru, Ciprian Laurentiu AU - Patru CL AUID- ORCID: 0000-0003-3672-5989 AD - Department 8 Mother and Child, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. LA - eng PT - Journal Article PT - Review DEP - 20261020 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704618 OTO - NOTNLM OT - causation OT - clinical judgement OT - healthcare systems OT - medical liability OT - medical malpractice OT - obstetrics and gynaecology OT - patient autonomy OT - patient safety OT - standard of care COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:36 PHST- 2026/09/21 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - healthcare14203531 [pii] AID - healthcare-14-03531 [pii] AID - 10.3390/healthcare14203531 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 20;14(20):3531. doi: 10.3390/healthcare14203531. PMID- 42940558 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 19 TI - Clinical Judgment of Home-Visit Nurses with an Extended Scope of Practice in Japan: A Qualitative Study. LID - 10.3390/healthcare14203513 [doi] LID - 3513 AB - Background/Objectives: The super-aged society and shortening of hospital stays in Japan have increased the importance of the role of home-visit nurses. The 2015 Specific Medical Acts Training Program qualified registered nurses with an extended scope of practice (RNs-ESP) to perform specified medical procedures based on physician-prepared protocols. Although the procedural activities of RNs-ESP have been documented, their underlying clinical judgments remain unclear. This study aimed to clarify the clinical judgments of home-visit RNs-ESP in Japan. Methods: This qualitative, descriptive study used participant observation and semi-structured interviews. Five home-visit RNs-ESP with at least 2 years of post-training experience participated. Data were collected from 32 clinical cases during home visits and analyzed using Mayring's qualitative content analysis, utilizing Tanner's clinical judgment model as a conceptual framework for operational definition. Results: The clinical judgments of RNs-ESP were classified into five categories and 18 subcategories: "Interpretation of pathological conditions and prediction of changes", "Directing daily living assistance based on general conditions", "Construction of the home care system in anticipation of pathological changes", "Optimization of pharmacotherapy tailored to pathological conditions and daily life", and "Selection of medical procedures tailored to daily life". RNs-ESP integrated medical reasoning with patients' life perspectives to design and implement optimal, highly individualized care plans. Conclusions: The clinical judgments of RNs-ESP are characterized by the integration of pathological assessment and daily life needs, supported by autonomous intervention authority. These judgments are essential nursing practices that balance continuous safe medical treatment with patient dignity and quality of life in home care. FAU - Gotoh, Tomomi AU - Gotoh T AUID- ORCID: 0009-0002-7304-6600 AD - Graduate School of Nursing, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8672, Japan. AD - Seikyo-Ukima Clinic, Tokyo-Hokuto Health Co-operative Association, 3-22-1 Ukima, Kita-ku, Tokyo 115-0051, Japan. FAU - Saiki, Masatoshi AU - Saiki M AUID- ORCID: 0000-0001-6247-965X AD - Graduate School of Nursing, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8672, Japan. FAU - Ouchi, Motoshi AU - Ouchi M AD - Graduate School of Nursing, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8672, Japan. FAU - Sakai, Ikuko AU - Sakai I AD - Graduate School of Nursing, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8672, Japan. LA - eng GR - FBK230601011/France BED Co., Ltd. (Japan)/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704268 OTO - NOTNLM OT - Japan OT - advanced practice nursing OT - clinical judgment OT - clinical reasoning OT - extended scope of practice OT - home-visit nursing OT - specified medical acts COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:36 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - healthcare14203513 [pii] AID - healthcare-14-03513 [pii] AID - 10.3390/healthcare14203513 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 19;14(20):3513. doi: 10.3390/healthcare14203513. PMID- 42940557 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 19 TI - Changes in Functional and Health-Related Outcomes After a Disability-Delay Program Among Community-Dwelling Older Adults. LID - 10.3390/healthcare14203512 [doi] LID - 3512 AB - Background: Frailty, sarcopenia, and cognitive decline are associated with reduced functional independence and participation among older adults. Occupational therapy uses multidomain and participation-focused approaches to support functional independence and healthy aging; however, evidence regarding functional and health-related changes among community-dwelling older adults participating in structured multidomain programs remains limited. This study aimed to describe pre-post changes in functional and health-related outcomes and to explore associations among concurrent changes in these measures. Methods: This uncontrolled retrospective pre-post program evaluation analyzed routinely collected data from 60 community-dwelling older adults who participated in a 12-week occupational therapist-led multidomain community program at five community sites. The program was delivered once weekly for 2 h and incorporated cognitive, physical, and daily activity-related components. Functional ability, frailty, sarcopenia risk, grip strength, cognitive function, and health-related quality of life were assessed at baseline and program completion. Pre-post differences were examined using paired-samples t-tests, and separate age-adjusted linear regression models were used to examine associations between individual clinical change variables and concurrent changes in functional outcomes. Results: Pre-post differences were observed in activities of daily living, frailty, sarcopenia risk, grip strength, cognitive function, and health-related quality of life. In separate exploratory age-adjusted regression models, changes in frailty (SOF), sarcopenia risk (SARC-F), and cognitive function (SPMSQ) were associated with concurrent changes in selected functional outcomes. These associations were considered exploratory and should not be interpreted as evidence of independent prediction or causality. Conclusions: Pre-post changes in multiple functional and health-related outcomes were observed among community-dwelling older adults participating in the 12-week occupational therapist-led multidomain program. Given the uncontrolled retrospective pre-post design, the observed changes cannot be attributed to the program, and the associations among concurrent changes should not be interpreted as causal. These findings may inform occupational therapy practice and the development of community-based multidomain programs aimed at supporting functional independence and participation in daily life. Further prospective controlled studies are needed to confirm these findings and determine whether such programs produce clinically meaningful changes in functional and health-related outcomes. FAU - Chen, Yu-Chung AU - Chen YC AUID- ORCID: 0009-0008-1534-3483 AD - Department of Physical Medicine and Rehabilitation, Chi Mei Hospital, Liouying, Tainan 736402, Taiwan. FAU - Li, Yi-Chun AU - Li YC AD - Department of Occupational Therapy, College of Medicine, I-Shou University, Kaohsiung 824005, Taiwan. FAU - Hong, Rong-Bin AU - Hong RB AD - Department of Physical Medicine and Rehabilitation, Chi Mei Hospital, Liouying, Tainan 736402, Taiwan. FAU - Shih, Yi-Chen AU - Shih YC AD - Department of Physical Medicine and Rehabilitation, Chi Mei Hospital, Liouying, Tainan 736402, Taiwan. FAU - Chang, Jer-Hao AU - Chang JH AUID- ORCID: 0000-0002-0055-4957 AD - Institute of Allied Health Sciences, College of Medicine, National Cheng Kung University, Tainan 701401, Taiwan. AD - Department of Occupational Therapy, College of Medicine, National Cheng Kung University, Tainan 701401, Taiwan. LA - eng GR - CLFHR11316/Chi Mei Hospital, Liouying/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704230 OTO - NOTNLM OT - activities of daily living OT - community-dwelling older adults OT - functional independence OT - multidomain intervention OT - occupational therapy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:36 PHST- 2026/09/18 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - healthcare14203512 [pii] AID - healthcare-14-03512 [pii] AID - 10.3390/healthcare14203512 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 19;14(20):3512. doi: 10.3390/healthcare14203512. PMID- 42940543 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 18 TI - Screen Culture's Association with Generation Z Adolescents' Posture and Well-Being in Egypt: Cross-Sectional Study. LID - 10.3390/healthcare14203498 [doi] LID - 3498 AB - Background: Egyptian Generation Z adolescents are heavily engaged with screens for several purposes, including education, entertainment, social media, and gaming. This raises red flags about how screen culture may affect sleep quality, posture-related habits, and health-related quality of life. Objectives: To examine how different types and amounts of weekday and weekend screen time are associated with symptoms of insomnia, body awareness of postural habits, and health-related quality of life among Egyptian Generation Z adolescents. Materials and Methods: A cross-sectional English-language online survey was completed by a self-selected convenience sample of Egyptian adolescents aged 13-18 years from multiple governorates; 530 participants were included in the analysis. Participants reported total screen time, the Insomnia Severity Index, Body Awareness of Postural Habits questionnaire, and the Pediatric Quality of Life Inventory. Multiple linear regression models were adjusted for age, gender, body mass index, level of education, and place of residence. Results: After false-discovery-rate adjustment across 30 comparisons, 13 activity-specific associations met the significance threshold. Higher ISI scores were associated with weekday job or internship-related use, weekday and weekend entertainment use, and weekday and weekend social-media use. Lower Q-BAPHYP scores were associated with weekday and weekend entertainment and social-media use. Lower PedsQL scores were associated with weekday job or internship-related use, weekday entertainment use, and weekday and weekend social-media use. The models explained limited variability in the outcomes. Conclusions: The screen use of Egyptian Generation Z adolescents is associated with insomnia, postural awareness, and overall quality of life; additional unmeasured factors probably influence such outcomes as well. FAU - Kora, Alaa Noureldeen AU - Kora AN AUID- ORCID: 0000-0002-7446-1083 AD - Department of Health Rehabilitation Sciences, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk 71491, Saudi Arabia. FAU - Alshehre, Yousef M AU - Alshehre YM AUID- ORCID: 0000-0003-2374-7716 AD - Department of Health Rehabilitation Sciences, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk 71491, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261018 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704529 OTO - NOTNLM OT - Generation Z OT - adolescents OT - insomnia OT - postural habits OT - quality of life OT - screen time COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:36 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - healthcare14203498 [pii] AID - healthcare-14-03498 [pii] AID - 10.3390/healthcare14203498 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 18;14(20):3498. doi: 10.3390/healthcare14203498. PMID- 42940539 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 17 TI - Predicting Demographic Contraction as an Upstream Signal for Health-System Planning: A Persistence-Corrected Machine-Learning Framework. LID - 10.3390/healthcare14203494 [doi] LID - 3494 AB - BACKGROUND/OBJECTIVES: Demographic contraction changes the scale and geography of healthcare demand while weakening the fiscal and workforce base that sustains services. The general objective was to evaluate a persistence-corrected one-year-ahead demographic forecasting framework and to determine whether it can provide a defensible upstream signal for health-system planning; the primary model forecasts demographic change rather than health-system capacity itself. METHODS: The analytic sample retained 672 of 4616 documented country-year observations (14.6%) from 47 countries (2000-2020), representing a coverage-selected panel with sufficiently complete cross-domain data. Requiring consecutive observations produced a 522-row dynamic panel from 45 countries and 167 one-year-ahead forecasts for 2016-2020. DPC-GB learned deviations from the current population-growth rate under expanding-window validation. Analyses compared momentum and cross-domain feature sets, matched gradient-boosting hyperparameters for direct and correction targets, lagged structural covariates, held country groups out of training, and used country-cluster uncertainty estimates. Incident-onset probabilities were examined only as a supplementary exploratory analysis. RESULTS: Among the feature specifications, momentum plus non-health predictors had the lowest observed RMSE (0.20491), followed by momentum only (0.20503), the full DPC-GB model (0.20531), and momentum plus health predictors (0.20680); persistence had RMSE 0.21920. The full model's observed RMSE was 6.34% lower than persistence, but the country-cluster bootstrap interval for the absolute difference [-0.0038, 0.0366] crossed zero (one-sided p = 0.0968), so improved forecasting across countries was not established. With identical gradient-boosting hyperparameters, direct level prediction had RMSE 0.2259 versus 0.2053 for persistence correction, although their country-cluster difference interval included zero. After Holm correction across four exploratory transfer endpoints, evidence remained for fertility forecasting; hospital-bed density was not significant after adjustment (adjusted p = 0.072). Only one incident contraction occurred among 136 eligible forecasts, precluding validation of probability calibration, discrimination, or operating thresholds. CONCLUSIONS: DPC-GB is a retrospective proof-of-concept for persistence correction in demographic forecasting, with possible downstream relevance to health-system planning. These data do not establish improved point forecasting across countries. Generalizability is constrained by complete-case selection. Momentum plus non-health predictors yielded the lowest observed RMSE, but its small numerical advantage over momentum only does not establish superiority; the three-feature momentum-only specification provides a parsimonious alternative to the full 42-feature model. Incident-risk probabilities remain exploratory and cannot support validated early-warning or threshold claims. FAU - Kim, Da Hee AU - Kim DH AD - Department of Medical Management, Chodang University, 380, Muan-ro, Muan-eup, Muan-gun 58530, Jeollanam-do, Republic of Korea. FAU - Na, Hyung Jong AU - Na HJ AUID- ORCID: 0009-0002-7259-4840 AD - Department of Accounting and Taxation, Semyung University, Jecheon 27136, Chungcheongbuk-do, Republic of Korea. LA - eng PT - Journal Article DEP - 20261017 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704099 OTO - NOTNLM OT - demographic contraction OT - fertility OT - health policy OT - health-system planning OT - hospital-bed density OT - machine learning OT - permutation importance OT - persistence correction COIS- The authors declare that they have no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:36 PHST- 2026/09/06 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - healthcare14203494 [pii] AID - healthcare-14-03494 [pii] AID - 10.3390/healthcare14203494 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 17;14(20):3494. doi: 10.3390/healthcare14203494. PMID- 42940536 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 17 TI - Complications and Hospital Resource Utilization Associated with ECMO in COVID-19 ARDS with Acute Kidney Injury: Insights from United States National Inpatient Sample, 2020-2022. LID - 10.3390/healthcare14203490 [doi] LID - 3490 AB - BACKGROUND: Acute kidney injury (AKI) is a common complication of COVID-19-associated acute respiratory distress syndrome (ARDS), yet the impact of extracorporeal membrane oxygenation (ECMO) on outcomes in this high-risk population remains unclear. METHODS: We performed a retrospective, survey-weighted study using the National Inpatient Sample (NIS) database from 2020-2022 to compare outcomes among adults hospitalized with COVID-19-associated ARDS and AKI managed with invasive mechanical ventilation (IMV) alone versus IMV and ECMO. The primary outcome was in-hospital mortality, while secondary outcomes included renal replacement therapy (RRT), complications, hospital length of stay (LOS), and healthcare utilization. RESULTS: Among 198,395 hospitalizations with COVID-19 ARDS complicated by AKI, 4730 received IMV and ECMO. ECMO use was not associated with statistically significantly lower adjusted in-hospital mortality (adjusted odds ratio [aOR] 0.96, 95% CI 0.81-1.12). However, ECMO was associated with greater odds of RRT, intracranial hemorrhage, disseminated intravascular coagulation, cardiogenic shock, vasopressor use, tracheostomy, acute liver failure, deep vein thrombosis, ischemic stroke and ICU-acquired weakness. ECMO was also associated with longer LOS and higher hospitalization charges. CONCLUSIONS: Among hospitalizations with COVID-19-associated ARDS and AKI, ECMO was not associated with statistically significantly lower adjusted in-hospital mortality but was associated with markedly higher complication burden, longer hospitalization, and substantially greater healthcare resource utilization. FAU - Arumairaj, Antony AU - Arumairaj A AUID- ORCID: 0000-0002-3824-1657 AD - Woodhull Medical Center, NYU Langone, Brooklyn, NY 11206, USA. FAU - Dhorajiya, Poojaben AU - Dhorajiya P AUID- ORCID: 0009-0000-8331-4088 AD - University of Kansas Hospital, Kansas City, KS 66160, USA. FAU - Dhanani, Dili AU - Dhanani D AUID- ORCID: 0009-0006-1365-1963 AD - University of Kansas Hospital, Kansas City, KS 66160, USA. FAU - Venkataswamy Reddy, Jyothika AU - Venkataswamy Reddy J AUID- ORCID: 0009-0005-5084-920X AD - Chamarajanagar Institute of Medical Sciences, Chamarajanagar 571313, Karnataka, India. FAU - Mariswamy Arun Kumar, Abhishek Kumar AU - Mariswamy Arun Kumar AK AUID- ORCID: 0009-0006-1299-6185 AD - Department of Internal Medicine, Wright State University, Dayton, OH 45458, USA. FAU - Tashrifwala, Fatema Ali Asgar AU - Tashrifwala FAA AUID- ORCID: 0000-0002-7537-9719 AD - Stamford Hospital, Stamford, CT 06902, USA. FAU - Prajapati, Vipulkumar AU - Prajapati V AD - School of Medicine, Virginia Commonwealth University, Richmond, VA 23298, USA. FAU - Palkamsetti, Sireesha AU - Palkamsetti S AD - Saint Agnes Medical Center, Fresno, CA 93720, USA. FAU - Tamvada, Dheera AU - Tamvada D AD - Golden Valley Health Centers, Merced, CA 95341, USA. FAU - Patel, Digantkumar AU - Patel D AUID- ORCID: 0009-0001-3922-6352 AD - Springfield Clinic, Springfield, IL 62703, USA. FAU - Korpu, Divya AU - Korpu D AUID- ORCID: 0000-0002-1409-5923 AD - Mayo Clinic Health System, Eau Claire, WI 54703, USA. FAU - Mittal, Jayesh AU - Mittal J AUID- ORCID: 0009-0006-2054-8833 AD - Asante Rogue Regional Medical Center, Medford, OR 97504, USA. LA - eng PT - Journal Article DEP - 20261017 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704019 OTO - NOTNLM OT - COVID-19 OT - acute kidney injury OT - acute respiratory distress syndrome OT - extracorporeal membrane oxygenation OT - mechanical ventilation OT - mortality OT - renal replacement therapy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:36 PHST- 2026/08/18 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - healthcare14203490 [pii] AID - healthcare-14-03490 [pii] AID - 10.3390/healthcare14203490 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 17;14(20):3490. doi: 10.3390/healthcare14203490. PMID- 42940532 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 17 TI - Comparison of Midwife-Led and Obstetrician-Led Care Models: Associations with Maternal Bonding and Breastfeeding Self-Efficacy. LID - 10.3390/healthcare14203487 [doi] LID - 3487 AB - Background/Objectives: The postpartum period is important for mother-infant bonding and breastfeeding self-efficacy. This study compared these outcomes between women receiving midwife-led and obstetrician-led intrapartum care. Methods: A prospective longitudinal observational study was conducted in two Lithuanian hospitals between September 2022 and July 2024. Women with low-risk births (n = 349) were assessed at 1-3 days and 6-8 weeks postpartum using the MIBS and BSES-SF. Results: In unadjusted comparisons, total MIBS scores showed a more stable pattern in the MW-led group (between-group difference in change, p = 0.004), and total BSES-SF scores increased within the MW-led group (p < 0.001). In adjusted analyses, the care setting was not independently associated with change in total MIBS or total BSES-SF scores. At 6-8 weeks postpartum, care setting (p = 0.041) and parity (p = 0.017) were associated with total BSES-SF scores. Conclusions: Selected unadjusted outcomes showed more favourable patterns in the MW-led setting, but adjusted analyses did not support an independent association between care setting and change in either principal outcome. Since each model was represented by a different hospital, observed associations cannot be attributed specifically to the professional care model. FAU - Nagineviciute, Milda AU - Nagineviciute M AUID- ORCID: 0009-0003-2212-1823 AD - Department of Nursing, Faculty of Nursing, Lithuanian University of Health Sciences, LT 44307 Kaunas, Lithuania. FAU - Bartuseviciene, Egle AU - Bartuseviciene E AUID- ORCID: 0000-0002-7043-4635 AD - Department of Obstetrics and Gynecology, Faculty of Medicine, Lithuanian University of Health Sciences, LT 44307 Kaunas, Lithuania. FAU - Blazeviciene, Aurelija AU - Blazeviciene A AUID- ORCID: 0000-0003-2130-5615 AD - Department of Nursing, Faculty of Nursing, Lithuanian University of Health Sciences, LT 44307 Kaunas, Lithuania. LA - eng PT - Journal Article DEP - 20261017 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704451 OTO - NOTNLM OT - breastfeeding self-efficacy OT - midwife-led care OT - mother-infant bonding OT - patient-reported outcomes COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:36 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - healthcare14203487 [pii] AID - healthcare-14-03487 [pii] AID - 10.3390/healthcare14203487 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 17;14(20):3487. doi: 10.3390/healthcare14203487. PMID- 42940528 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 16 TI - Effectiveness of Non-Pharmacological Interventions for Fatigue in Long COVID: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. LID - 10.3390/healthcare14203483 [doi] LID - 3483 AB - Background: Persistent fatigue is one of the most common and disabling symptoms of long COVID, yet no pharmacological treatments are currently established. Non-drug strategies are increasingly used, but evidence directly comparing these approaches remains limited. This systematic review and meta-analysis evaluated the effects of non-pharmacological interventions on fatigue in adults with long COVID and explored potential sources of heterogeneity. Materials and Methods: Following PRISMA 2020 and the Cochrane Handbook, we searched four databases from inception to December 2025 for RCTs comparing non-pharmacological interventions with control conditions in adults with WHO-defined long COVID. The primary outcome was pre-specified as fatigue severity; no secondary outcomes were pre-specified. Methodological quality was assessed using the RoB 2 tool. Random-effects models were used to pool SMDs, with exploratory subgroup, sensitivity, and publication bias analyses. Results: Thirteen RCTs (1276 participants) were included. Non-pharmacological interventions significantly reduced fatigue [SMD = -0.43, 95% CI: -0.68 to -0.18, p < 0.001], with substantial heterogeneity (I(2) = 72.1%). Subgroup analyses showed that exercise training yielded significant benefit [SMD = -0.64, 95% CI: -1.11 to -0.18, p = 0.006], whereas cognitive behavioural therapy, non-invasive neuromodulation, and nutritional supplementation did not reach statistical significance. Interventions lasting >/=8 weeks were associated with greater improvement (between-subgroup p = 0.027). Sensitivity analyses confirmed robustness, and no publication bias was detected. Conclusions: Non-pharmacological interventions, particularly exercise-based programmes, are associated with reduced fatigue in people with long COVID, with interventions lasting >/=8 weeks showing greater average improvements. Exercise-based rehabilitation currently has the most consistent evidence base among the evaluated modalities, although the difference in effects between intervention modalities was not statistically significant (p = 0.286). Further high-quality trials are required to determine the comparative effectiveness of emerging approaches and to identify optimal treatment strategies. FAU - Bi, Yanze AU - Bi Y AUID- ORCID: 0009-0002-5605-3884 AD - College of Integrated Chinese and Western Medicine, Changchun University of Traditional Chinese Medicine, Changchun 130117, China. FAU - Yang, Bo AU - Yang B AD - Second Department of Pulmonary Diseases, Affiliated Hospital of Changchun University of Chinese Medicine, Changchun 130021, China. FAU - Chen, Siyu AU - Chen S AD - College of Integrated Chinese and Western Medicine, Changchun University of Traditional Chinese Medicine, Changchun 130117, China. FAU - Li, Qingrong AU - Li Q AD - College of Integrated Chinese and Western Medicine, Changchun University of Traditional Chinese Medicine, Changchun 130117, China. FAU - Xu, Rong AU - Xu R AD - College of Integrated Chinese and Western Medicine, Changchun University of Traditional Chinese Medicine, Changchun 130117, China. FAU - Zhang, Wenbo AU - Zhang W AD - College of Integrated Chinese and Western Medicine, Changchun University of Traditional Chinese Medicine, Changchun 130117, China. FAU - Yang, Yunyi AU - Yang Y AD - College of Integrated Chinese and Western Medicine, Changchun University of Traditional Chinese Medicine, Changchun 130117, China. FAU - Zou, Wenshuang AU - Zou W AD - Department of Gastroenterology, Affiliated Hospital to Changchun University of Traditional Chinese Medicine, Changchun 130021, China. FAU - Hu, Shaodan AU - Hu S AD - Department of Pneumology, Affiliated Hospital of Changchun University of Traditional Chinese Medicine, Changchun 130021, China. LA - eng GR - 20230203078SF/the Department of Science and Technology of Jilin Province/ PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704095 OTO - NOTNLM OT - long COVID OT - meta-analysis OT - non-pharmacological interventions OT - randomized controlled trial COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:36 PHST- 2026/07/04 00:00 [received] PHST- 2026/09/02 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - healthcare14203483 [pii] AID - healthcare-14-03483 [pii] AID - 10.3390/healthcare14203483 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 16;14(20):3483. doi: 10.3390/healthcare14203483. PMID- 42940514 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 15 TI - Current Perspectives and Effectiveness of Fertility Preservation in Cancer Patients-Data from Serbian National Referral Center. LID - 10.3390/healthcare14203469 [doi] LID - 3469 AB - BACKGROUND/OBJECTIVES: Despite numerous fertility-sparing treatments, there are still uncertainties. We present the experience and effectiveness of the oncofertility program from the University Clinical Center of Serbia. METHODS: Study included all malignancy patients undergoing fertility-sparing treatment during a 10-year period. For all patients, we registered malignancy type, therapy type, course and duration, the response to therapy, complications and recurrence, and overall survival. All attempted and achieved pregnancies were recorded. We noted all pregnancy complications and gestational week at pregnancy end and calculated live-birth rates. RESULTS: Fifteen patients with early-stage cervical cancer (squamocellular 11; adenocarcinoma 4) had abdominal radical trachelectomy. Outcomes of our patients were excellent, but just three patients delivered healthy children. In total, 87 patients with early-stage endometrial malignancy (atypical hyperplasia 24, intraepithelial neoplasm 24, carcinoma 39) received hormonal therapy. Complete remission was achieved at 74.7% but only nine achieved pregnancy. Moreover, 52 patients with stage I borderline ovarian tumors were treated. The overall survival rate was 100% and the pregnancy rate was 44.8%. We also treated 39 patients with non-epithelial ovarian cancers (sexcord-stromal tumors 22, malignant germ cell tumors 17). The overall survival rate was 87.2%, but we had just seven successful pregnancies. In our institution, oocytes were cryopreserved in 63, embryos in 6, and ovarian tissue in 8 patients, out of which two achieved pregnancy and one had a term delivery of a healthy child. CONCLUSIONS: Our findings support the feasibility of favorable oncologic and reproductive outcomes in carefully selected oncofertility patients following current guidelines. FAU - Stefanovic, Katarina AU - Stefanovic K AD - Clinic of Obstetrics and Gynecology, University Clinical Centre of Serbia, Dr. Koste Todorovica 26, 11000 Belgrade, Serbia. AD - Medical Faculty, University of Belgrade, Dr. Subotica 8, 11000 Belgrade, Serbia. FAU - Beleslin, Aleksandra AU - Beleslin A AUID- ORCID: 0000-0002-2407-8975 AD - Clinic of Obstetrics and Gynecology, University Clinical Centre of Serbia, Dr. Koste Todorovica 26, 11000 Belgrade, Serbia. FAU - Pilic, Igor AU - Pilic I AD - Clinic of Obstetrics and Gynecology, University Clinical Centre of Serbia, Dr. Koste Todorovica 26, 11000 Belgrade, Serbia. AD - Medical Faculty, University of Belgrade, Dr. Subotica 8, 11000 Belgrade, Serbia. FAU - Likic Ladjevic, Ivana AU - Likic Ladjevic I AUID- ORCID: 0000-0001-9743-2528 AD - Clinic of Obstetrics and Gynecology, University Clinical Centre of Serbia, Dr. Koste Todorovica 26, 11000 Belgrade, Serbia. AD - Medical Faculty, University of Belgrade, Dr. Subotica 8, 11000 Belgrade, Serbia. FAU - Stojnic, Jelena AU - Stojnic J AUID- ORCID: 0000-0003-0359-9568 AD - Clinic of Obstetrics and Gynecology, University Clinical Centre of Serbia, Dr. Koste Todorovica 26, 11000 Belgrade, Serbia. AD - Medical Faculty, University of Belgrade, Dr. Subotica 8, 11000 Belgrade, Serbia. FAU - Micic, Jelena AU - Micic J AUID- ORCID: 0000-0001-5686-0307 AD - Clinic of Obstetrics and Gynecology, University Clinical Centre of Serbia, Dr. Koste Todorovica 26, 11000 Belgrade, Serbia. AD - Medical Faculty, University of Belgrade, Dr. Subotica 8, 11000 Belgrade, Serbia. FAU - Stefanovic, Aleksandar AU - Stefanovic A AD - Clinic of Obstetrics and Gynecology, University Clinical Centre of Serbia, Dr. Koste Todorovica 26, 11000 Belgrade, Serbia. AD - Medical Faculty, University of Belgrade, Dr. Subotica 8, 11000 Belgrade, Serbia. FAU - Didanovic, Aleksandra AU - Didanovic A AD - Clinic of Obstetrics and Gynecology, University Clinical Centre of Serbia, Dr. Koste Todorovica 26, 11000 Belgrade, Serbia. FAU - Stepanovic, Anja AU - Stepanovic A AD - Clinic of Obstetrics and Gynecology, University Clinical Centre of Serbia, Dr. Koste Todorovica 26, 11000 Belgrade, Serbia. FAU - Dotlic, Jelena AU - Dotlic J AUID- ORCID: 0000-0001-9683-9561 AD - Clinic of Obstetrics and Gynecology, University Clinical Centre of Serbia, Dr. Koste Todorovica 26, 11000 Belgrade, Serbia. AD - Medical Faculty, University of Belgrade, Dr. Subotica 8, 11000 Belgrade, Serbia. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704148 OTO - NOTNLM OT - Serbia OT - obstetric outcome OT - oncofertility OT - oncologic outcome COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:36 PHST- 2026/08/19 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - healthcare14203469 [pii] AID - healthcare-14-03469 [pii] AID - 10.3390/healthcare14203469 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 15;14(20):3469. doi: 10.3390/healthcare14203469. PMID- 42940493 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 15 TI - The Impact of an Integrated County-Township-Village Care Model on Type 2 Diabetes Management in Resource-Constrained Areas in China: A Synthetic Control Method. LID - 10.3390/healthcare14203450 [doi] LID - 3450 AB - Objective: The objective of this study is to evaluate county-specific changes in standardized management and glycemic control associated with an integrated county-township-village care model among patients with type 2 diabetes mellitus (T2DM) in resource-constrained areas of China. Methods: Three intervention counties that implemented an integrated county-township-village care model in 2019 were included. County-level quarterly data from 2017 to 2022 were extracted from electronic health record systems. Two outcome measures were assessed: the standardized management rate and the glycemic control rate among patients with T2DM. Synthetic controls were constructed from non-intervention counties within the same prefecture-level city. Sensitivity analyses excluding the highest-weight donor were conducted. Results: The synthetic controls approximated pre-intervention characteristics and outcome trajectories, although fit varied across counties and outcomes. Following implementation, mean standardized management rates exceeded their synthetic counterparts by 4.57, 3.96, and 4.35 percentage points in Counties Q, Y, and F, respectively. Placebo tests provided suggestive evidence for Counties Q and Y (p = 0.100 and p = 0.111) but limited evidence for County F (p = 0.750). Mean glycemic control rates exceeded their synthetic counterparts by 4.61 and 3.81 percentage points in Counties Q and F, respectively, compared with 0.83 percentage points in County Y. Estimated differences remained positive after excluding the highest-weight donor, although their magnitudes were sensitive to donor selection in some models. Conclusions: The integrated county-township-village care model was associated with improvements in selected T2DM management outcomes, with patterns varying across counties. Our findings suggest potential value in strengthening collaboration between county hospitals and primary health care institutions in comparable resource-constrained settings. FAU - Wang, Jingbo AU - Wang J AD - School of Public Health, Fudan University, Shanghai 200032, China. AD - Center for Project Supervision and Management, National Health Commission, Beijing 100044, China. FAU - Xing, Xing AU - Xing X AD - School of Public Health, Peking University, Beijing 100191, China. FAU - Liu, Shimeng AU - Liu S AD - School of Public Health, Fudan University, Shanghai 200032, China. AD - National Health Commission Key Laboratory of Health Technology Assessment, Fudan University, Shanghai 200032, China. FAU - Wei, Yan AU - Wei Y AD - School of Public Health, Fudan University, Shanghai 200032, China. AD - National Health Commission Key Laboratory of Health Technology Assessment, Fudan University, Shanghai 200032, China. FAU - Chen, Yingyao AU - Chen Y AD - School of Public Health, Fudan University, Shanghai 200032, China. AD - National Health Commission Key Laboratory of Health Technology Assessment, Fudan University, Shanghai 200032, China. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704070 OTO - NOTNLM OT - glycemic control OT - integrated care OT - primary health care OT - synthetic control method OT - type 2 diabetes mellitus COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:36 PHST- 2026/07/28 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - healthcare14203450 [pii] AID - healthcare-14-03450 [pii] AID - 10.3390/healthcare14203450 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 15;14(20):3450. doi: 10.3390/healthcare14203450. PMID- 42940488 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 14 TI - Infection Control Knowledge, Job Stress, and Self-Efficacy in Relation to Infection Control Performance Among Intensive Care Unit Registered Nurses: A Descriptive Cross-Sectional Study. LID - 10.3390/healthcare14203443 [doi] LID - 3443 AB - BACKGROUND/OBJECTIVES: Healthcare-associated infections remain a critical patient-safety concern in intensive care units (ICUs), where invasive procedures and immunocompromised patients elevate infection risk. Infection control knowledge, job stress, and self-efficacy have each been linked to infection control performance, but few studies have examined these factors simultaneously among ICU registered nurses (RNs). This study aimed to investigate ICU RNs' infection control knowledge, infection control job stress, self-efficacy, and infection control performance, and to identify factors associated with infection control performance. METHODS: This descriptive cross-sectional study surveyed 191 ICU RNs (of 219 recruited) with >/=6 months of ICU experience at two tertiary hospitals in the Republic of Korea (December 2024-January 2025) using structured, validated questionnaires. Data were analyzed with descriptive statistics, independent t-tests, one-way ANOVA (Scheffe/Games-Howell post hoc), Pearson correlation, and hierarchical multiple regression (SPSS 22.0). RESULTS: Mean scores were 19.16 +/- 1.07 (of 20) for infection control knowledge, 3.23 +/- 0.67 (of 5) for infection control job stress, 3.62 +/- 0.53 (of 5) for self-efficacy, and 4.82 +/- 0.26 (of 5) for infection control performance. Infection control performance was positively correlated with knowledge (r = 0.151, p = 0.037), job stress (r = 0.161, p = 0.026), and self-efficacy (r = 0.178, p = 0.014). Hierarchical regression showed that current ICU department (surgical, cardiac, emergency, trauma, and general ICU vs. internal medicine ICU as reference), infection control job stress (beta = 0.188, p = 0.008), and self-efficacy (beta = 0.157, p = 0.022) significantly predicted infection control performance, together explaining 17.4% of the variance (F = 5.995, p < 0.001); infection control knowledge was a nominally significant predictor in this model (beta = 0.164, p = 0.016), but this association was not robust to sensitivity analyses (Spearman rho = 0.034, p = 0.636; heteroscedasticity-robust regression p = 0.095) or to a covariate-adjusted robustness check (p = 0.091), and should be interpreted with caution. CONCLUSIONS: Infection control job stress, self-efficacy, and current department were robustly associated with infection control performance among ICU RNs across all sensitivity analyses; infection control knowledge showed a comparatively weaker, fragile association that did not survive rank-based, heteroscedasticity-robust, or covariate-adjusted sensitivity checks. Effect sizes were small to moderate overall, and the final model explained only 17.4% of variance. Department-tailored education and organizational support strategies-channeling job stress constructively and strengthening self-efficacy through hands-on training and mentoring-may help sustain and improve infection control performance; recommendations regarding knowledge-related training should be treated as preliminary given the fragility of this association. FAU - Lee, Ji Soo AU - Lee JS AUID- ORCID: 0009-0007-0569-0150 AD - Department of Nursing, Graduate School, Wonkwang University, Iksan 54538, Republic of Korea. FAU - Jeong, Eun Young AU - Jeong EY AD - College of Nursing, Wonkwang University, Iksan 54538, Republic of Korea. LA - eng GR - Wonkwang University/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704700 OTO - NOTNLM OT - healthcare-associated infection OT - infection control job stress OT - infection control knowledge OT - infection control performance OT - intensive care unit OT - registered nurse OT - self-efficacy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:36 PHST- 2026/08/05 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - healthcare14203443 [pii] AID - healthcare-14-03443 [pii] AID - 10.3390/healthcare14203443 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 14;14(20):3443. doi: 10.3390/healthcare14203443. PMID- 42940487 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 14 TI - Demographic Characteristics and Evolution of Catheterization Patterns Among Cancer Patients with Peripherally Inserted Central Catheters in Huangshi, China: A Single-Center Retrospective Study. LID - 10.3390/healthcare14203442 [doi] LID - 3442 AB - Background: We aim to describe PICC placement characteristics, patient demographics, and temporal trends in insertion site selection among cancer patients at a tertiary hospital, and to provide real-world evidence on PICC practice patterns. Methods: Data from 1760 cancer patients who underwent PICC placement between January 2018 and December 2025 were analyzed. Descriptive statistics were used to summarize demographic characteristics and catheter parameters. Between-group comparisons were performed using the Kruskal-Wallis H test and Dunn's test (with Bonferroni correction). Effect sizes (epsilon(2), Cohen's r), 95% confidence intervals (bootstrap method), and the Cochran-Armitage trend test for annual proportions were calculated. A multivariable general linear model (GLM) was employed to identify independent factors associated with catheter insertion length. Results: Among the 1760 patients, the right basilic vein was the most frequently used site. During 2018-2023, the basilic vein predominated; however, from 2024, the proportion of median cubital vein insertions increased substantially and became predominant by 2025. The annual proportions of both veins changed significantly over the study period (Cochran-Armitage trend test, p < 0.001 for both). Digestive and respiratory malignancies were the most common tumor types. Catheter insertion length and upper arm circumference exhibited statistically significant differences across age and diagnostic groups, albeit with small effect sizes. In the multivariable analysis, after adjusting for venipuncture site and other covariates, the magnitude of catheter length differences between the respiratory/thoracic tumor group and other major tumor groups was attenuated, indicating that the choice of anatomical site partially confounded the initial unadjusted findings. Conclusions: During 2024-2025, PICC placement shifted from the basilic vein to the median cubital vein, reflecting a notable change in real-world practice. Although the subgroup differences in catheter length and arm circumference were statistically significant, the clinical significance was limited. These descriptive findings do not directly guide nursing protocols. Future studies need to incorporate data on operator experience, ultrasound utilization, and complications to elucidate the mechanisms and clinical impact of this transition. FAU - Xie, Jumin AU - Xie J AUID- ORCID: 0000-0002-4493-4548 AD - Hubei Key Laboratory of Renal Disease Occurrence and Intervention, School of medicine, Hubei Polytechnic University, Huangshi 435003, China. FAU - Lv, Xinyi AU - Lv X AD - Hubei Key Laboratory of Renal Disease Occurrence and Intervention, School of medicine, Hubei Polytechnic University, Huangshi 435003, China. FAU - Wang, Xuening AU - Wang X AD - Hubei Key Laboratory of Renal Disease Occurrence and Intervention, School of medicine, Hubei Polytechnic University, Huangshi 435003, China. FAU - Jiang, Zejing AU - Jiang Z AD - Hubei Key Laboratory of Renal Disease Occurrence and Intervention, School of medicine, Hubei Polytechnic University, Huangshi 435003, China. FAU - Xie, Yuxuan AU - Xie Y AD - Hubei Key Laboratory of Renal Disease Occurrence and Intervention, School of medicine, Hubei Polytechnic University, Huangshi 435003, China. FAU - Ren, Changhui AU - Ren C AD - Department of Science and Education, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Huangshi 435000, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704004 OTO - NOTNLM OT - PICC placement patterns OT - cancer patients OT - peripherally inserted central catheter OT - retrospective study OT - temporal trends OT - vein selection COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:36 PHST- 2026/08/13 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - healthcare14203442 [pii] AID - healthcare-14-03442 [pii] AID - 10.3390/healthcare14203442 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 14;14(20):3442. doi: 10.3390/healthcare14203442. PMID- 42940477 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 14 TI - Understanding How to Make Type 2 Diabetes Prevention Programs More Inclusive: An Equity-Oriented Qualitative Case Study of Small Steps for Big Changes. LID - 10.3390/healthcare14203432 [doi] LID - 3432 AB - Background/Objectives: Diabetes prevention programs (DPPs) reduce type 2 diabetes (T2D) risk, yet their reach often remains misaligned with the populations most affected by social and structural diabetes risk. This study examined inclusivity barriers within Small Steps for Big Changes (SSBC), a Canadian DPP developed at the University of British Columbia, through an in-depth qualitative case study of a participant whose age, ethnicity and gender identity differed from the program's typical participant profile. Methods: The participant was purposively selected for the present case analysis from individuals enrolled in SSBC and completed the standard 4-week intervention, consisting of one-on-one motivational interviewing-informed counseling, supervised exercise, fitness-tracker- and mobile-app-supported self-monitoring, and planned long-term follow-up. Data were collected from two semi-structured Zoom interviews, six session-day journal entries and two optional non-session journal entries between July and September 2022. Data were analyzed in NVivo using interpretive description. Results: The participant was a young South Asian adult who self-identified as male. Five themes were identified: lack of access, limitations and barriers, lack of support, mental health implications, and SSBC-related takeaways. Barriers included culturally irrelevant nutrition resources, the university food environment, cost constraints, a one-year wait for dietetic care, isolation from peers managing prediabetes, and prediabetes-related guilt and stress. Supportive, non-judgmental coaching strengthened accountability and motivation but did not fully address culture-, age-, student-life-, and mental-health-related needs. Conclusions: This case identifies practical equity targets for DPP redesign, including culturally adaptable dietary resources, peer support, inclusive coaching training and mental health-responsive prevention care. FAU - Der, Sarah AU - Der S AUID- ORCID: 0009-0008-1123-2225 AD - School of Health and Exercise Sciences, Faculty of Health and Social Development, University of British Columbia, Okanagan Campus, Kelowna, BC V1V 1V7, Canada. FAU - Cranston, Kaela AU - Cranston K AD - School of Health and Exercise Sciences, Faculty of Health and Social Development, University of British Columbia, Okanagan Campus, Kelowna, BC V1V 1V7, Canada. FAU - Jung, Mary AU - Jung M AUID- ORCID: 0000-0002-2360-0952 AD - School of Health and Exercise Sciences, Faculty of Health and Social Development, University of British Columbia, Okanagan Campus, Kelowna, BC V1V 1V7, Canada. LA - eng GR - GR022307/CAPMC/CIHR/Canada PT - Journal Article DEP - 20261014 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704304 OTO - NOTNLM OT - South Asian OT - cultural tailoring OT - diabetes prevention program OT - health equity OT - motivational interviewing OT - prediabetes OT - qualitative case study OT - social determinants of health OT - type 2 diabetes OT - young adults COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:36 PHST- 2026/07/30 00:00 [received] PHST- 2026/09/03 00:00 [revised] PHST- 2026/09/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - healthcare14203432 [pii] AID - healthcare-14-03432 [pii] AID - 10.3390/healthcare14203432 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 14;14(20):3432. doi: 10.3390/healthcare14203432. PMID- 42940468 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 13 TI - Psychological Frailty in Later Life: A Critical Narrative Review of Integrative Interventions and the Proposed RESERVE-7 Heuristic. LID - 10.3390/healthcare14203423 [doi] LID - 3423 AB - BACKGROUND: Psychological frailty may describe reduced capacity to adapt to stress in later life, but definitions vary and its added clinical value is unknown. METHODS: We conducted a critical narrative review of MEDLINE via PubMed (1 January 2015 to 29 August 2026), citation chains, and selected guidance. The original core query returned 114 records; the focused reconciliation added construct-specific reports. Forty-nine sources inform this synthesis. RESULTS: Trials have measured psychological-frailty domains, and D-SCOPE permitted a psychological-domain threshold as one of several enrollment routes. Freitag and Schmidt reported small short-term improvements in frailty and mental health; D-SCOPE reported no significant six-month within-group change in psychological frailty; and MIND-STEP reported a psychological-frailty risk difference of -13.87 percentage points at 12 weeks (95% CI -25.04 to -2.69). These measures are not interchangeable with our proposed affective-adaptive definition. We found no trial that exclusively recruited participants using a validated version of that proposed construct. Adjacent evidence concerns defined symptoms and functional outcomes. We propose RESERVE-7 as an unvalidated heuristic for identifying goals, reversible drivers, an initial feasible intervention, treatment burden, and follow-up. CONCLUSIONS: Construct-specific intervention findings deserve explicit consideration, but they do not validate psychological reserve or RESERVE-7. Prospective validation is needed before adoption. FAU - Ibrahim, Fatma Magdi AU - Ibrahim FM AD - Faculty of Nursing, Mansoura University, Mansoura 35516, Egypt. AD - RAK College of Nursing, RAK Medical and Health Sciences University, Ras Al Khaimah P.O. Box 11172, United Arab Emirates. FAU - Shahrour, Ghada AU - Shahrour G AUID- ORCID: 0000-0002-6929-3361 AD - Faculty of Nursing, Jordan University of Science and Technology, Irbid 22110, Jordan. FAU - Elbarbary, Khaled AU - Elbarbary K AD - Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704219 OTO - NOTNLM OT - aging OT - geropsychology OT - integrative care OT - lifestyle intervention OT - mindfulness OT - person-centered care OT - psychological frailty OT - psychological reserve OT - psychotherapy OT - social connection COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:36 PHST- 2026/08/30 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - healthcare14203423 [pii] AID - healthcare-14-03423 [pii] AID - 10.3390/healthcare14203423 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 13;14(20):3423. doi: 10.3390/healthcare14203423. PMID- 42940459 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 13 TI - When the Body Meets Space: The Contribution of Interoception and Emotional Object-Location Memory as Predictors of Health-Related Quality of Life in Aging. LID - 10.3390/healthcare14203414 [doi] LID - 3414 AB - BACKGROUND/OBJECTIVES: Adaptive functioning involves the integration of bodily states with emotional information from the external environment. Interoceptive functioning and spatial cognition may contribute to this integration, but their joint associations with health-related quality of life (HRQoL) across adulthood remain insufficiently understood. METHODS: We examined associations between multidimensional interoceptive functioning, emotional object-location memory, and HRQoL in younger adults (n = 43) and older adults (n = 38). Participants completed an immersive virtual-reality Object Location Memory task assessing object recognition (What), spatial location memory (Where), and object-location binding (What + Where) for positive, negative, and neutral stimuli. Interoceptive functioning was assessed using the Multidimensional Assessment of Interoceptive Awareness (MAIA) and the Body Perception Questionnaire (BPQ), and HRQoL was assessed using the SF-36. For each SF-36 domain and age group, interoceptive predictors were screened using bootstrap-based LASSO selection and subsequently entered into multiple regression linear models together with the prespecified spatial-memory predictors. Model robustness was further examined using bootstrap, leave-one-out cross-validation, and permutation testing. RESULTS: The regression analyses identified associations between interoceptive functioning, emotional object-location memory, and HRQoL. In older adults, selected dimensions of interoceptive functioning, particularly those related to attention regulation, adaptive engagement with bodily sensations, and confidence in bodily signals, were associated with several HRQoL domains. Emotional object-location memory also showed domain-specific associations, with positive- and negative-valence measures contributing differently across HRQoL outcomes. In younger adults, selected associations were also observed between interoceptive functioning, emotional object-location memory, and HRQoL, including associations involving neutral and positive object-location information. CONCLUSIONS: The findings provide exploratory evidence that specific dimensions of interoceptive functioning and emotionally modulated object-location memory are associated with selected aspects of self-reported HRQoL, with distinct patterns of associations emerging in younger and older adults. FAU - Iuliano, Sabrina AU - Iuliano S AUID- ORCID: 0009-0005-9214-6799 AD - Department of Psychology, University of Campania "Luigi Vanvitelli", Viale Ellittico 31, 81100 Caserta, Italy. FAU - Nunziata, Scila AU - Nunziata S AUID- ORCID: 0009-0003-2163-1418 AD - Department of Psychology, University of Campania "Luigi Vanvitelli", Viale Ellittico 31, 81100 Caserta, Italy. FAU - Pasciucco, Maria Rosaria AU - Pasciucco MR AUID- ORCID: 0009-0008-2081-0859 AD - Department of Neuroscience, Imaging and Clinical Sciences, "Gabriele d'Annunzio" University of Chieti and Pescara, Via dei Vestini, 33, 66100 Chieti, Italy. FAU - Ferri, Francesca AU - Ferri F AD - Department of Neuroscience, Imaging and Clinical Sciences, "Gabriele d'Annunzio" University of Chieti and Pescara, Via dei Vestini, 33, 66100 Chieti, Italy. AD - Institute for Advanced Biomedical Technologies, "Gabriele d'Annunzio" University of Chieti and Pescara, Via dei Vestini, 33, 66100 Chieti, Italy. FAU - Costantini, Marcello AU - Costantini M AUID- ORCID: 0000-0002-0504-1296 AD - Department of Neuroscience, Imaging and Clinical Sciences, "Gabriele d'Annunzio" University of Chieti and Pescara, Via dei Vestini, 33, 66100 Chieti, Italy. AD - Institute for Advanced Biomedical Technologies, "Gabriele d'Annunzio" University of Chieti and Pescara, Via dei Vestini, 33, 66100 Chieti, Italy. FAU - Ruggiero, Gennaro AU - Ruggiero G AUID- ORCID: 0000-0002-3940-6740 AD - Department of Psychology, University of Campania "Luigi Vanvitelli", Viale Ellittico 31, 81100 Caserta, Italy. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704354 OTO - NOTNLM OT - aging OT - emotional processing OT - functional ability OT - health-related quality of life OT - interoceptive awareness OT - object-location memory OT - spatial memory COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:36 PHST- 2026/08/07 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - healthcare14203414 [pii] AID - healthcare-14-03414 [pii] AID - 10.3390/healthcare14203414 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 13;14(20):3414. doi: 10.3390/healthcare14203414. PMID- 42940455 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 13 TI - Self-Reported Provision of Community Pharmacy Services and Perceived Barriers in Saudi Arabia: A Cross-Sectional Survey. LID - 10.3390/healthcare14203410 [doi] LID - 3410 AB - Background/Objectives: Community pharmacists can broaden access to medication-related, preventive, and screening services, but implementation in Saudi Arabia remains uneven. This study described self-reported counselling and extended-service provision, perceived barriers, and statistical relationships between pharmacist- and practice-level characteristics and reported service delivery. Methods: A cross-sectional online survey included 600 community pharmacists in Saudi Arabia; an independent pilot included 50 pharmacists. The instrument assessed seven counselling practices, eight extended services, and thirteen barriers. Group comparisons used chi-square or Fisher-Freeman-Halton exact tests with false-discovery-rate adjustment, and threshold-specific logistic regression models evaluated service provision. Results: Agreement with counselling practices ranged from 78.7% to 91.3%. Frequent provision was low for vaccination (6.0%), cholesterol screening (8.2%), glucose screening (10.5%), and blood pressure assessment (11.7%). Leading barriers were insufficient public awareness (66.3%), limited patient time (64.5%), lack of medical-record access (58.3%), inadequate privacy (56.5%), and weak primary-care integration (54.7%). Educational qualification did not show an independent adjusted relationship with extended-service provision. Selected service outcomes differed across practice-setting categories, and exploratory analyses identified an inverse statistical relationship between perceived barriers and extended-service provision. Conclusions: Routine counselling was commonly reported, whereas most extended services were infrequent. Expanding community pharmacy services requires attention to workflow, staffing, privacy, patient information, public awareness, referral pathways, and health-system integration. FAU - Alharbi, Emtenan M AU - Alharbi EM AUID- ORCID: 0009-0009-0842-9534 AD - Department of Clinical Pharmacy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia. FAU - Alharbi, Manal M AU - Alharbi MM AUID- ORCID: 0000-0001-8757-3490 AD - Department of Optometry, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia. FAU - Aldarwesh, Amal Qassim AU - Aldarwesh AQ AUID- ORCID: 0000-0002-0922-8541 AD - Department of Optometry, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Saudi Arabia. FAU - Alotaibi, Moureq AU - Alotaibi M AD - Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia. FAU - Bafail, Arwa Omar AU - Bafail AO AD - College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia. FAU - Alsaegh, Rawabi Rashed AU - Alsaegh RR AD - College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia. LA - eng GR - ORF-2026-2201/King Saud University/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704151 OTO - NOTNLM OT - Saudi Arabia OT - community pharmacy OT - cross-sectional study OT - health services OT - implementation barriers OT - pharmaceutical care OT - screening services COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:36 PHST- 2026/08/11 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - healthcare14203410 [pii] AID - healthcare-14-03410 [pii] AID - 10.3390/healthcare14203410 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 13;14(20):3410. doi: 10.3390/healthcare14203410. PMID- 42940433 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 11 TI - Comparative Effectiveness of Aquatic Versus Land-Based Exercise on Pain, Physical Function, and Quality of Life in People with Knee Osteoarthritis: An Updated Systematic Review of Randomised Controlled Trials. LID - 10.3390/healthcare14203388 [doi] LID - 3388 AB - Background/Objectives: Knee osteoarthritis (OA) causes pain, physical function limitations, and reduced quality of life. This updated systematic review compared the effectiveness of aquatic exercise (AE) versus land-based exercise (LBE) on these outcomes in adults with knee OA. Methods: Randomised controlled trials (RCTs) published between September 2018 and July 2025 were included if they compared supervised AE with LBE. PubMed, Web of Science, Scopus, and PEDro were searched. Methodological quality was assessed using the PEDro scale, and the certainty of evidence was evaluated using GRADE. Owing to substantial clinical and methodological heterogeneity in participant characteristics, intervention content and dose, outcome measures, and reporting methods, quantitative pooling was not considered clinically meaningful; therefore, a structured synthesis without meta-analysis was conducted. Results: Nine RCTs enrolling 700 participants were included, of whom 661 contributed directly to the AE versus LBE comparison. Between-group effects varied across outcomes and studies. Differences in pain measured using the VAS generally ranged from -1.80 to -0.32 scale units in favour of AE; however, clinical importance remained uncertain because applicable thresholds were not available for all measures and the confidence interval around the largest estimate included effects below the contextual benchmark. Evidence for physical function and quality of life was inconsistent, and several confidence intervals included no between-group effect or were wide. Although selected outcomes favoured AE, the observed differences were generally small or imprecise, and their clinical importance was frequently uncertain. The certainty of evidence ranged from very low to high and was predominantly low or very low. Conclusions: The evidence included in this update does not establish consistent clinically important superiority of AE over LBE for people with knee OA. Although some between-group estimates favoured AE, most effects were small or imprecise, and confidence in the findings was limited. Exercise modality should therefore be selected according to individual characteristics, preferences, accessibility, and clinical context. Further well-designed multicentre trials with standardised outcome reporting, longer-term follow-up, and comprehensive adherence assessment are needed. FAU - Amaral, Mariana AU - Amaral M AD - Coimbra Health School, Polytechnic University of Coimbra, Rua 5 de Outubro, 3045-043 Coimbra, Portugal. FAU - Correia, Beatriz AU - Correia B AUID- ORCID: 0009-0003-4347-9760 AD - Coimbra Health School, Polytechnic University of Coimbra, Rua 5 de Outubro, 3045-043 Coimbra, Portugal. FAU - Oliveira, Mariana AU - Oliveira M AD - Coimbra Health School, Polytechnic University of Coimbra, Rua 5 de Outubro, 3045-043 Coimbra, Portugal. FAU - Santos, Beatriz AU - Santos B AUID- ORCID: 0009-0006-4371-980X AD - Coimbra Health School, Polytechnic University of Coimbra, Rua 5 de Outubro, 3045-043 Coimbra, Portugal. FAU - Ferreira, Ricardo Maia AU - Ferreira RM AUID- ORCID: 0000-0003-1596-9685 AD - Coimbra Health School, Polytechnic University of Coimbra, Rua 5 de Outubro, 3045-043 Coimbra, Portugal. AD - Social Sciences, Education and Sport Department, Polytechnic Institute of Maia, N2i, 4475-690 Maia, Portugal. AD - Sport Physical Activity and Health Research & Innovation Center (SPRINT), 4960-320 Melgaco, Portugal. FAU - Goncalves, Rui Soles AU - Goncalves RS AUID- ORCID: 0000-0002-6118-0338 AD - Coimbra Health School, Polytechnic University of Coimbra, Rua 5 de Outubro, 3045-043 Coimbra, Portugal. AD - H&TR-Health & Technology Research Center, Coimbra Health School, Polytechnic University of Coimbra, Rua 5 de Outubro, 3045-043 Coimbra, Portugal. LA - eng PT - Journal Article PT - Review DEP - 20261011 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704367 OTO - NOTNLM OT - aquatic exercise OT - knee osteoarthritis OT - land-based exercise OT - pain OT - physical function OT - quality of life COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/11 CRDT- 2026/10/27 10:36 PHST- 2026/06/29 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - healthcare14203388 [pii] AID - healthcare-14-03388 [pii] AID - 10.3390/healthcare14203388 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 11;14(20):3388. doi: 10.3390/healthcare14203388. PMID- 42940430 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 11 TI - Telemedicine in Disaster Medicine-Use of Paramedics as Tele-Consultants to Guide Personnel with Limited Medical Training at the Disaster Site. LID - 10.3390/healthcare14203385 [doi] LID - 3385 AB - Background/Objectives: Disaster medicine is frequently constrained by shortages of highly qualified medical personnel. Telemedicine may extend advanced expertise to less-qualified responders, but previous disaster telemedicine models have predominantly relied on physicians as remote consultants. This study compared patient care outcomes under telemedical support provided by experienced Paramedics with tele-physician support, conventional on-site physician care, and EMT-only care. Methods: A prospective, simulation-based, controlled quasi-experimental study was conducted during a 7.5-h mass casualty exercise in Hamburg, Germany. Seven treatment tents were assigned to four groups: on-site physician (CONT), EMT-only care (IV 1), tele-physician support (IV 2), and tele-Paramedic support (IV 3). Outcomes comprised time to first treatment order, indicated treatments addressed, analgesia management, and patient contact frequency by the most highly qualified team member. Results: A total of 151 simulated patients were included. Time to first treatment order did not differ significantly between groups (p = 0.09). The proportion of indicated treatments addressed differed significantly (p < 0.001), with rates of 56% in CONT, 8% in IV 1, 57% in IV 2, and 51% in IV 3. Analgesia was addressed in 68%, 7%, 59%, and 60% of patients with an indication, respectively (p < 0.001). Increasing pain intensity significantly increased the likelihood of analgesia in CONT, IV 2, and IV 3 but not in IV 1. Recorded patient contact frequencies were descriptively higher in the telemedicine groups, although contacts were operationally defined differently between telemedicine and non-telemedicine models. Conclusions: Tele-physician and tele-Paramedic support showed similar observed performance across selected outcomes to conventional physician-led care and substantially better rates of indicated treatments addressed and analgesia provision than EMT-only care. The findings indicate that experienced Paramedics may represent a valuable additional resource for telemedical disaster response. Larger studies are needed to confirm these results, define the appropriate scope of practice, and evaluate tiered consultation models in which tele-Paramedics provide first-line support and tele-physicians remain available for escalation of complex cases. FAU - Walter, Ulrike AU - Walter U AUID- ORCID: 0009-0006-3977-8567 AD - Faculty of Medicine, RWTH Aachen University, 52062 Aachen, Germany. FAU - Weber, Lukas AU - Weber L AUID- ORCID: 0009-0004-3051-4376 AD - Faculty of Medicine, RWTH Aachen University, 52062 Aachen, Germany. FAU - Czaplik, Michael AU - Czaplik M AUID- ORCID: 0000-0002-7118-7728 AD - Faculty of Medicine, RWTH Aachen University, 52062 Aachen, Germany. AD - Docs in Clouds Telecare GmbH, 52074 Aachen, Germany. FAU - Larmann, Jan AU - Larmann J AUID- ORCID: 0000-0003-3365-4572 AD - Faculty of Medicine, RWTH Aachen University, 52062 Aachen, Germany. FAU - Follmann, Andreas AU - Follmann A AUID- ORCID: 0000-0001-6817-2472 AD - Faculty of Medicine, RWTH Aachen University, 52062 Aachen, Germany. FAU - Muller, Anna AU - Muller A AUID- ORCID: 0000-0001-8855-6481 AD - Faculty of Medicine, RWTH Aachen University, 52062 Aachen, Germany. LA - eng GR - 061d-2024/Federal Office of Civil protection and Disaster Assistance/ PT - Journal Article DEP - 20261011 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704386 OTO - NOTNLM OT - civil protection OT - disaster medicine OT - mass casualty incident OT - simulation OT - tele-Paramedic OT - tele-consultation OT - tele-physician OT - telemedicine COIS- M.C. (CEO, shareholder) is affiliated with Docs in Clouds TeleCare GmbH, a company developing telemedicine software. A.M. was employed by University Hospital RWTH Aachen and seconded part-time to the German Federal Office of Civil Protection and Disaster Assistance during the study, where she was involved in its implementation. The other authors declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/11 CRDT- 2026/10/27 10:36 PHST- 2026/08/20 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - healthcare14203385 [pii] AID - healthcare-14-03385 [pii] AID - 10.3390/healthcare14203385 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 11;14(20):3385. doi: 10.3390/healthcare14203385. PMID- 42940429 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 11 TI - Synchronous Physical Telerehabilitation Versus Face-to-Face Rehabilitation in Axial Spondyloarthritis: A Focused Narrative Review. LID - 10.3390/healthcare14203384 [doi] LID - 3384 AB - Background and Objectives: Exercise is central to axSpA management, but whether live remote delivery preserves effects of the same face-to-face program is uncertain. Methods: Five databases were searched until 9 September 2026; registry, reference, and citation searches ended on 11 September 2026. Reports from one trial were linked; contextual studies were illustrative. Results: Of 214 database records, 123 unique records were screened, and 11 reports representing eight studies were selected from the five databases. A ClinicalTrials.gov record and doctoral thesis linked to the Karaca trial were obtained separately and were not counted among those 11 database-selected reports. One assessor-blinded RCT compared the same individualized clinical Pilates program by videoconference or in clinic. Fifty participants were randomized, 46 assessed at eight weeks, and 40 at six months. Median BASFI improvement was 3.0 versus 2.5 points at eight weeks (p = 0.587) and 2.0 versus 2.0 points at six months (p = 0.838). Mann-Whitney U tests used alpha = 0.017; between-group effect estimates and 95% confidence intervals were unavailable. All-randomized attendance was 93.8% versus 94.0%. Five participants reported transient symptoms; three serious events were judged unrelated, and no intervention-related serious adverse event was reported. Conclusions: Remote delivery was implementable with high short-term attendance among digitally eligible participants. This trial does not establish equivalence, non-inferiority, preserved effect, broad accessibility, or a safety profile. Adequately powered non-inferiority trials are needed. FAU - Ciumarnean, Lorena AU - Ciumarnean L AUID- ORCID: 0000-0002-8491-8817 AD - Department of Internal Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. FAU - Gavris, Madalina Daiana AU - Gavris MD AUID- ORCID: 0009-0002-7829-3627 AD - Department of Hematology, The Oncology Institute Prof. Dr. Ion Chiricuta, 400015 Cluj-Napoca, Romania. FAU - Vesa, Stefan Cristian AU - Vesa SC AUID- ORCID: 0000-0003-4549-3526 AD - Department of Pharmacology, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. FAU - Milaciu, Mircea Vasile AU - Milaciu MV AUID- ORCID: 0000-0002-5087-9282 AD - Department of Internal Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. FAU - Orasan, Olga Hilda AU - Orasan OH AUID- ORCID: 0000-0003-4435-142X AD - Department of Internal Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. FAU - Para, Ioana AU - Para I AD - Department of Internal Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. FAU - Alexescu, Teodora Gabriela AU - Alexescu TG AUID- ORCID: 0000-0003-2756-5784 AD - Department of Internal Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. FAU - Vlad, Irina Maria AU - Vlad IM AD - Department of Neurology, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. FAU - Dogaru, Gabriela Bombonica AU - Dogaru GB AD - Department of Medical Rehabilitation, Iuliu Hatieganu University of Medicine and Pharmacy, 400012 Cluj-Napoca, Romania. LA - eng PT - Journal Article PT - Review DEP - 20261011 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704107 OTO - NOTNLM OT - ankylosing spondylitis OT - axial spondyloarthritis OT - exercise therapy OT - face-to-face rehabilitation OT - physiotherapy OT - synchronous telerehabilitation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/11 CRDT- 2026/10/27 10:36 PHST- 2026/08/11 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - healthcare14203384 [pii] AID - healthcare-14-03384 [pii] AID - 10.3390/healthcare14203384 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 11;14(20):3384. doi: 10.3390/healthcare14203384. PMID- 42940415 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 9 TI - Multidimensional Factors and Predictors of Vulnerability and Frailty in Older Adults: A Systematic Review. LID - 10.3390/healthcare14203370 [doi] LID - 3370 AB - BACKGROUND: Population ageing is characterized by rising multimorbidity and multifaceted vulnerability. While vulnerability is inherently a biopsychosocial state, clinical research predominantly operationalizes risk through physical frailty phenotypes, constraining holistic geriatric care. Identifying the interplay across biological, functional, and psychosocial domains is essential for comprehensive risk evaluation. OBJECTIVES: To synthesize multidimensional factors associated with vulnerability and frailty in adults aged >/=65, categorizing cross-sectional correlates and prospective longitudinal predictors to inform multidimensional assessment. METHODS: Following PRISMA 2020 guidelines, systematic searches were conducted across PubMed, CINAHL, Scopus, Web of Science, and Google Scholar (2020-2025). Independent reviewers performed screening, selection, extraction, and Joanna Briggs Institute (JBI) critical appraisal (PROSPERO: CRD420251039796). RESULTS: Forty studies (22 cross-sectional, 17 cohort, 1 quasi-experimental) comprising 77,229 cumulative participant observations across community, outpatient, inpatient, and residential care settings were synthesized. Thirty-seven candidate determinants were mapped across five domains: biological/clinical, psychological, socioeconomic/relational, lifestyle, and self-care profile. Advanced age, multimorbidity, and polypharmacy were the most frequent clinical factors; prospective cohorts specifically linked high anticholinergic burden and functional dependence in complex activities to incident frailty. Depressive symptoms, cognitive decline, low education, financial strain, and social isolation consistently delineated vulnerable states. CONCLUSIONS: Vulnerability in older adults is a multifaceted construct requiring assessment tools that extend beyond isolated physical markers. Rather than a definitive feature set, these identified factors provide an evidence-based pool of candidate variables that may inform future risk-stratification and predictive modeling initiatives, supporting integrated, person-centered care. FAU - Pino, Helder AU - Pino H AUID- ORCID: 0000-0003-1632-6948 AD - Comprehensive Health Research Centre (CHRC), Universidade de Evora, 7000-801 Evora, Portugal. AD - Unidade Local de Saude do Algarve (ULS Algarve), 8000-386 Faro, Portugal. FAU - Ramos, Ana AU - Ramos A AUID- ORCID: 0000-0002-4661-0731 AD - Comprehensive Health Research Centre (CHRC), Universidade de Evora, 7000-801 Evora, Portugal. AD - Nursing Research Innovation and Development Centre of Lisbon (CIDNUR), Escola Superior de Enfermagem da Universidade de Lisboa, 1600-096 Lisbon, Portugal. FAU - Fonseca, Cesar AU - Fonseca C AUID- ORCID: 0000-0001-6975-612X AD - Comprehensive Health Research Centre (CHRC), Universidade de Evora, 7000-801 Evora, Portugal. AD - Escola Superior de Enfermagem Sao Joao de Deus, Universidade de Evora, 7000-811 Evora, Portugal. FAU - Alves, Elisabete AU - Alves E AUID- ORCID: 0000-0002-7738-0887 AD - Comprehensive Health Research Centre (CHRC), Universidade de Evora, 7000-801 Evora, Portugal. AD - Escola Superior de Enfermagem Sao Joao de Deus, Universidade de Evora, 7000-811 Evora, Portugal. FAU - Coelho, Anabela AU - Coelho A AUID- ORCID: 0000-0002-1750-1229 AD - Comprehensive Health Research Centre (CHRC), Universidade de Evora, 7000-801 Evora, Portugal. AD - Escola Superior de Enfermagem Sao Joao de Deus, Universidade de Evora, 7000-811 Evora, Portugal. AD - Global Health and Tropical Medicine, Instituto de Higiene e Medicina Tropical, Universidade NOVA de Lisboa, 1099-085 Lisbon, Portugal. LA - eng PT - Journal Article PT - Review DEP - 20261009 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704491 OTO - NOTNLM OT - elderly OT - frailty OT - geriatric assessment OT - predictors OT - systematic review OT - vulnerability COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:36 PHST- 2026/08/25 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - healthcare14203370 [pii] AID - healthcare-14-03370 [pii] AID - 10.3390/healthcare14203370 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 9;14(20):3370. doi: 10.3390/healthcare14203370. PMID- 42940414 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 9 TI - Overweight, Obesity, and Musculoskeletal Symptom Burden in Chile: A Population-Based Cross-Sectional Study. LID - 10.3390/healthcare14203369 [doi] LID - 3369 AB - Background/Objectives: Musculoskeletal symptoms (MSS) are among the leading causes of pain and functional limitation worldwide, yet population-level evidence linking excess body weight to MSS in Latin America remains scarce. This study examined the association between body mass index (BMI) and the prevalence of non-traumatic MSS in the Chilean population. Methods: A cross-sectional secondary analysis was conducted using data from the 2009-2010 Chilean National Health Survey (n = 4689). MSS were assessed with the validated COPCORD CCQ-ILAR questionnaire, covering 12 anatomical regions over the previous seven days. BMI was categorized as <25, 25 to <30, and >/=30 kg/m(2). Survey-weighted negative binomial and logistic regression models were fitted, adjusting for age, sex, educational level, and area of residence. Results: Over half the sample (55.26%) reported at least one MSS. Each one-unit increase in BMI was associated with a 2.8% higher expected symptom count (CR = 1.028; 95% CI: 1.019-1.037). Compared with participants with BMI < 25 kg/m(2), those with overweight and obesity had 55.7% and 98.4% higher odds of reporting >/=1 MSS, respectively. The knee, shoulder, lower back, and ankle showed the steepest prevalence gradients across BMI strata. After correction for multiple comparisons, overweight remained associated with lower-back, finger, and knee symptoms, whereas obesity remained associated with shoulder, wrist, finger, knee, and ankle symptoms. Age and female sex were also independently associated with greater symptom burden. Conclusions: Higher BMI was positively associated with a greater overall MSS burden. Region-specific analyses identified robust associations in selected weight-bearing and upper-limb regions after correction for multiple comparisons. Because of the cross-sectional design, these findings do not establish that reducing BMI would reduce MSS. In addition, as the data were collected in 2009-2010, the prevalence estimates should not be interpreted as representing the current MSS burden in Chile. FAU - Lizana, Pablo A AU - Lizana PA AUID- ORCID: 0000-0002-9366-6930 AD - Laboratory of Epidemiology and Morphological Sciences, Instituto de Biologia, Pontificia Universidad Catolica de Valparaiso, Valparaiso 2373223, Chile. AD - Center for Interdisciplinary Research in Biomedicine, Biotechnology and Well-Being (CID3B), Pontificia Universidad Catolica de Valparaiso, Valparaiso 2373223, Chile. FAU - Lamas-Cirano, Camila AU - Lamas-Cirano C AD - Laboratory of Epidemiology and Morphological Sciences, Instituto de Biologia, Pontificia Universidad Catolica de Valparaiso, Valparaiso 2373223, Chile. FAU - Munoz-Caceres, Ana AU - Munoz-Caceres A AD - Laboratory of Epidemiology and Morphological Sciences, Instituto de Biologia, Pontificia Universidad Catolica de Valparaiso, Valparaiso 2373223, Chile. FAU - Lizama-Perez, Rodrigo AU - Lizama-Perez R AUID- ORCID: 0000-0002-6303-2662 AD - Departamento de Anatomia Normal y Medicina Legal, Facultad de Medicina, Universidad de Concepcion, Concepcion 4070386, Chile. FAU - Munoz-Cofre, Rodrigo AU - Munoz-Cofre R AUID- ORCID: 0000-0001-5690-8956 AD - Centro de Excelencia en Estudios Morfologicos y Quirurgicos, Universidad de La Frontera, Temuco 4811230, Chile. LA - eng GR - DI Regular 039.704/2025/Pontifical Catholic University of Valparaiso/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704324 OTO - NOTNLM OT - Chile OT - body mass index OT - health surveys OT - musculoskeletal symptoms OT - obesity OT - overweight OT - population health COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:36 PHST- 2026/08/09 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - healthcare14203369 [pii] AID - healthcare-14-03369 [pii] AID - 10.3390/healthcare14203369 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 9;14(20):3369. doi: 10.3390/healthcare14203369. PMID- 42940405 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9032 (Print) IS - 2227-9032 (Electronic) IS - 2227-9032 (Linking) VI - 14 IP - 20 DP - 2026 Oct 9 TI - Risk-Informed Complaint Management in Neurosurgery: Development and Temporal Validation of an Early-Warning Model for Dispute Escalation. LID - 10.3390/healthcare14203360 [doi] LID - 3360 AB - Background: Neurosurgery is a high-risk surgical specialty with substantial clinical uncertainty, procedural complexity, and potential for severe adverse outcomes. Although timely risk stratification after an initial complaint may facilitate structured communication, senior review, and coordinated dispute management, practical tools for estimating escalation risk at the first complaint remain limited. Methods: We conducted a single-center retrospective study of neurosurgical complaint events recorded at the Second Xiangya Hospital from January 2016 to December 2025. Time zero was the first documented complaint date. The endpoint was a final institutional severity grade of 7-10, denoting hospital-level handling or beyond, versus grades 1-6. Candidate predictors were restricted to information available on or before time zero and were selected by univariable screening followed by multivariable logistic regression. The sociodemographic support-needs profile was excluded from the model to avoid stigmatization and was used in fairness analyses. Results: Among 975 eligible complaint events, 161 (16.5%) reached high-grade escalation. Four predictors were retained: ICU admission, invasive manipulation failure, a pre-complaint communication-context factor, and anger at the first complaint. The model achieved moderate discrimination and calibration in the apparent cohort (AUC, 0.781; PR-AUC, 0.417; Brier score, 0.115) and similar performance in the 2023-2025 temporal test cohort (AUC, 0.759 [95% CI, 0.687-0.826]; PR-AUC, 0.364; Brier score, 0.119). At a threshold of 0.20, the positive predictive value in the test cohort was 0.333. Exploratory risk strata separated high-grade escalation rates from 5.8% in the low-risk group to 16.6% and 37.3% in the intermediate- and high-risk groups. Conclusions: Routinely available clinical and communication indicators, recorded by the time of the first complaint, can support risk-informed complaint management in neurosurgery. Because two of every three high-risk flags did not escalate, the model should support senior review and early communication rather than serve as a stand-alone classifier; external validation and local recalibration are required before use elsewhere. FAU - Zhang, Mingming AU - Zhang M AUID- ORCID: 0009-0006-3885-621X AD - Hunan Cerebrospinal Vascular Disease Diagnosis and Treatment Center, The Second Xiangya Hospital, Central South University, No. 139 Renmin Middle Road, Furong District, Changsha 410011, China. AD - Department of Neurosurgery, The Second Xiangya Hospital of Central South University, Changsha 410011, China. FAU - He, Jiarong AU - He J AD - Hunan Cerebrospinal Vascular Disease Diagnosis and Treatment Center, The Second Xiangya Hospital, Central South University, No. 139 Renmin Middle Road, Furong District, Changsha 410011, China. AD - Department of Neurosurgery, The Second Xiangya Hospital of Central South University, Changsha 410011, China. FAU - Chen, Yuquan AU - Chen Y AUID- ORCID: 0000-0001-5792-6566 AD - Faculty of Medicine, Nursing & Health Sciences, School of Public Health and Preventive Medicine, Monash University, Melbourne, VIC 3004, Australia. FAU - Cui, Jian AU - Cui J AD - Hunan Cerebrospinal Vascular Disease Diagnosis and Treatment Center, The Second Xiangya Hospital, Central South University, No. 139 Renmin Middle Road, Furong District, Changsha 410011, China. AD - Department of Neurosurgery, The Second Xiangya Hospital of Central South University, Changsha 410011, China. FAU - Zhou, Yu AU - Zhou Y AUID- ORCID: 0000-0001-9369-2082 AD - Department of Neurosurgery, The Second Xiangya Hospital of Central South University, Changsha 410011, China. AD - Hunan Guangxiu HiTech Life Technology Co., Ltd., Changsha 410217, China. AD - National Engineering Research Center of Human Stem Cells, Changsha 410205, China. LA - eng GR - 2026STI363/National Engineering Research Center of Science and Technology Information/ GR - 2026JJ40076/Natural Science Foundation of Hunan Province for Young Scholars/ GR - 2025JJ70010/Natural Science Foundation of Hunan Province/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Healthcare (Basel) JT - Healthcare (Basel, Switzerland) JID - 101666525 PMC - PMC13704672 OTO - NOTNLM OT - dispute escalation OT - early warning OT - neurosurgery OT - patient complaint OT - risk stratification COIS- Author Yu Zhou is employed by Hunan Guangxiu HiTech Life Technology Co., Ltd. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:36 PHST- 2026/09/06 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - healthcare14203360 [pii] AID - healthcare-14-03360 [pii] AID - 10.3390/healthcare14203360 [doi] PST - epublish SO - Healthcare (Basel). 2026 Oct 9;14(20):3360. doi: 10.3390/healthcare14203360. PMID- 42940400 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Development of Sustainable Extraction Processes for the Recovery of Antioxidants from Punica granatum L. Peel. LID - 10.3390/foods15203764 [doi] LID - 3764 AB - BACKGROUND: Pomegranate peel, a major by-product of the food industry, represents an underexploited source of valuable bioactive compounds. In the framework of a circular economy approach, this study aimed to develop a sustainable and solvent-free extraction strategy based on Saccharomyces cerevisiae fermentation for the recovery of antioxidant and antimicrobial phytochemicals from pomegranate peel waste. METHODS: Pomegranate peel was subjected to fermentation for 18, 24, 48, and 72 h, and the resulting extracts (YE_18h, YE_24h, YE_48h, and YE_72h), together with a conventionally obtained chemical extract (CE), were evaluated for their antioxidant activity. Based on its superior antioxidant activity, the extract obtained after 48 h of fermentation (YE_48h) was selected for ellagic acid quantification by HPLC and characterization by UHPLC-HRMS (Ultra-High-Performance Liquid Chromatography coupled to High-Resolution Mass Spectrometry). Antibacterial activity of YE_48h, in comparison with CE, was assessed against six Gram-positive and Gram-negative bacterial strains. RESULTS: Among the fermented samples, the extract obtained after 48 h of fermentation (YE_48h) exhibited the highest antioxidant activity, showing an IC(50) value lower than that of a conventional chemical extract. Quantitative HPLC analysis revealed a remarkable enhancement in ellagic acid content, with YE_48h containing approximately 18-fold higher levels of ellagic acid than the conventional chemical extraction. UHPLC-HRMS fingerprinting further demonstrated the presence of additional bioactive phenolic compounds, including punicalagin isomers I and II and pedunculagin isomer I, which are known for their antioxidant and antimicrobial properties. The antibacterial assay demonstrated that fermentation modulated the antimicrobial profile of the extracts in a strain-dependent manner. CONCLUSIONS: S. cerevisiae-mediated fermentation is an efficient and environmentally friendly approach for the valorization of pomegranate peel by-products, enhancing the release of bioactive compounds while generating extracts with promising antioxidant and selective antibacterial properties. FAU - Sagona, Simona AU - Sagona S AUID- ORCID: 0000-0003-1592-7522 AD - Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy. FAU - Margheriti, Francesco AU - Margheriti F AUID- ORCID: 0000-0003-1185-1954 AD - BioStructures Lab, Istituto Italiano di Tecnologia (IIT@NEST), 56127 Pisa, Italy. AD - Laboratorio NEST, Scuola Normale Superiore, Piazza San Silvestro 12, 56127 Pisa, Italy. FAU - Banti, Matteo AU - Banti M AD - Department of Pharmacy, University of Pisa, Via Bonanno 6, 56100 Pisa, Italy. FAU - Di Ricco, Fabio AU - Di Ricco F AUID- ORCID: 0009-0004-2161-6717 AD - Department of Pharmacy, University of Pisa, Via Bonanno 6, 56100 Pisa, Italy. FAU - Raffellini, Lorenzo AU - Raffellini L AUID- ORCID: 0009-0000-2728-0987 AD - Department of Pharmacy, University of Pisa, Via Bonanno 6, 56100 Pisa, Italy. FAU - Manera, Clementina AU - Manera C AD - Department of Pharmacy, University of Pisa, Via Bonanno 6, 56100 Pisa, Italy. AD - Center for Instrument Sharing, University of Pisa (CISUP), Lungarno Pacinotti 43, 56126 Pisa, Italy. AD - Interdepartmental Research Center Nutrafood, Nutraceuticals and Food for Health, University of Pisa, 56124 Pisa, Italy. FAU - Saccomanni, Giuseppe AU - Saccomanni G AD - Department of Pharmacy, University of Pisa, Via Bonanno 6, 56100 Pisa, Italy. AD - Interdepartmental Research Center Nutrafood, Nutraceuticals and Food for Health, University of Pisa, 56124 Pisa, Italy. FAU - Fratini, Filippo AU - Fratini F AUID- ORCID: 0000-0002-1717-2947 AD - Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy. AD - Interdepartmental Research Center Nutrafood, Nutraceuticals and Food for Health, University of Pisa, 56124 Pisa, Italy. FAU - De Leo, Marinella AU - De Leo M AUID- ORCID: 0000-0002-5544-8457 AD - Department of Pharmacy, University of Pisa, Via Bonanno 6, 56100 Pisa, Italy. AD - Center for Instrument Sharing, University of Pisa (CISUP), Lungarno Pacinotti 43, 56126 Pisa, Italy. AD - Interdepartmental Research Center Nutrafood, Nutraceuticals and Food for Health, University of Pisa, 56124 Pisa, Italy. FAU - Rapposelli, Simona AU - Rapposelli S AUID- ORCID: 0000-0003-0146-6358 AD - Department of Pharmacy, University of Pisa, Via Bonanno 6, 56100 Pisa, Italy. AD - Center for Instrument Sharing, University of Pisa (CISUP), Lungarno Pacinotti 43, 56126 Pisa, Italy. LA - eng GR - Laboratori Farmaceutici Asteriti Srl,/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704383 OTO - NOTNLM OT - Punica granatum peel OT - antibacterial activity OT - antioxidants OT - circular economy OT - fermentation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:36 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - foods15203764 [pii] AID - foods-15-03764 [pii] AID - 10.3390/foods15203764 [doi] PST - epublish SO - Foods. 2026 Oct 21;15(20):3764. doi: 10.3390/foods15203764. PMID- 42940393 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Thermoresponsive PNIPAM Gel-Coated PCL Nanofibers: Characterization, Vapor-Phase Release of Rosemary Essential Oil, and Activity Against Aspergillus flavus. LID - 10.3390/foods15203757 [doi] LID - 3757 AB - Rosemary essential oil (REO) was incorporated into electrospun poly(epsilon-caprolactone) (PCL) nanofibers, which were subsequently modified with a thermoresponsive poly(N-isopropylacrylamide) (PNIPAM)-gel coating using an immersion-based approach. The coated fibers exhibited temperature-dependent surface wetting behavior below and above the lower critical solution temperature (LCST) of PNIPAM. PNIPAM coating reduced water vapor transmission at 25 degrees C and increased the water vapor transmission ratio (WVTR(40)/WVTR(25)) from 1.65 to 2.31, whereas thickness-normalized water vapor permeability did not show a distinct LCST-dependent transition. Headspace analysis by HS-SPME-GC-MS showed increasing accumulation of REO volatiles with incubation time and temperature. After 48 h, PNIPAM coating reduced the total headspace amount of the selected volatile markers by approximately 7.2-fold at 25 degrees C and 4.9-fold at 40 degrees C. A significant coating x temperature interaction (p < 0.05) indicated that the coating modified the temperature dependence of volatile transfer, with the 40/25 degrees C ratio increasing from 1.65 in uncoated fibers to 2.41 in coated fibers. In non-contact antifungal assays against toxigenic Aspergillus flavus, REO-loaded fibers produced transient growth inhibition at 25 degrees C, whereas inhibition persisted throughout the 10-day incubation at 40 degrees C and was greater for the uncoated REO-loaded fibers. None of the fiber systems significantly affected AFB(1) accumulation at 25 degrees C, while AFB(1) was undetected in all samples, including the untreated control, at 40 degrees C. Overall, the PNIPAM-gel coating provided substantial retention of REO volatiles while introducing temperature-dependent modulation of their transfer to the gas phase, although the response did not represent a simple LCST-triggered release switch. FAU - Yener, Elif AU - Yener E AUID- ORCID: 0000-0002-8383-0581 AD - Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, Istanbul 34210, Turkiye. FAU - Karakas, Canan Yagmur AU - Karakas CY AD - Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, Istanbul 34210, Turkiye. FAU - Bozkurt, Fatih AU - Bozkurt F AD - Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, Istanbul 34210, Turkiye. FAU - Ozmen, Mehmet Murat AU - Ozmen MM AD - Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, Istanbul 34210, Turkiye. FAU - Ozer, Hayrettin AU - Ozer H AUID- ORCID: 0000-0002-9785-0030 AD - TUBITAK Marmara Research Center (MAM), Kocaeli 41470, Turkiye. FAU - Karadag, Ayse AU - Karadag A AUID- ORCID: 0000-0001-8615-7321 AD - Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, Istanbul 34210, Turkiye. LA - eng GR - 126O037/Turkiye Bilimsel ve Teknolojik Arastirma Kurumu/ GR - 119G001/Turkiye Bilimsel ve Teknolojik Arastirma Kurumu/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704677 OTO - NOTNLM OT - antifungal activity OT - electrospinning OT - headspace analysis OT - lower critical solution temperature (LCST) OT - poly(N-isopropylacrylamide) COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:36 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - foods15203757 [pii] AID - foods-15-03757 [pii] AID - 10.3390/foods15203757 [doi] PST - epublish SO - Foods. 2026 Oct 21;15(20):3757. doi: 10.3390/foods15203757. PMID- 42940391 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Comparative Analysis of Pre-Gelatinized Potato Starch Prepared by Different Drying Methods and Its Influence on Noodle Quality. LID - 10.3390/foods15203752 [doi] LID - 3752 AB - The present study investigated the effects of degree of starch gelatinization (DSG) and drying methods (FD: freeze-drying; OD: oven-drying) on the multi-scale structure of potato starch and its functionality in noodles. Potato starches with varying DSG (0-91.39%) were prepared by FD and OD and characterized by DSC, XRD, FTIR, RVA, and SEM. The short- and long-range ordered structure decreased significantly as DSG increased. Notably, OD samples showed significantly higher relative crystallinity than FD samples (18.55% vs. 10.90%). Peak, trough, and final viscosities increased, while breakdown decreased with increasing DSG. Microstructurally, FD samples exhibited fragmented flakes, whereas OD samples formed a dense and compacted solid matrix. In noodles, low-DSG FD starch significantly enhanced lightness (L* increased from 75.27 to 83.27), hardness (increased by 15.9%), gumminess, and chewiness, whereas this hardness-enhancing effect was reversed at higher DSG levels. Strong correlations were observed between DSG and structural parameters (r > 0.80). These findings demonstrate that the drying method fundamentally alters the molecular structure of partially gelatinized starch even at comparable DSG levels, and low-DSG FD starch enhanced noodle color and texture. The findings provide a scientific basis for the targeted selection of drying methods to tailor starch functionality for specific noodle applications. FAU - Xu, Fen AU - Xu F AUID- ORCID: 0000-0002-0447-3185 AD - Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China. AD - Tianjin International Joint Research and Development Center for Food Science and Engineering, Tianjin 300134, China. FAU - Wang, Chenxing AU - Wang C AD - Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China. AD - Tianjin International Joint Research and Development Center for Food Science and Engineering, Tianjin 300134, China. FAU - Duan, Hanyi AU - Duan H AUID- ORCID: 0009-0001-4942-0159 AD - Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China. AD - Tianjin International Joint Research and Development Center for Food Science and Engineering, Tianjin 300134, China. FAU - Li, Lingyi AU - Li L AUID- ORCID: 0000-0003-2476-2942 AD - Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China. AD - Tianjin International Joint Research and Development Center for Food Science and Engineering, Tianjin 300134, China. FAU - Liu, Jianfu AU - Liu J AD - Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China. AD - Tianjin International Joint Research and Development Center for Food Science and Engineering, Tianjin 300134, China. FAU - Hu, Honghai AU - Hu H AUID- ORCID: 0000-0003-4361-5470 AD - Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences (CAAS), Integrated Laboratory of Potato Staple Food Processing Technology of the Ministry of Agriculture and Rural Affairs, Key Laboratory of Agricultural Product Processing and Storage of the Ministry of Agriculture and Rural Affairs, Beijing 100193, China. FAU - Blecker, Christophe AU - Blecker C AUID- ORCID: 0009-0001-6871-5702 AD - Department of Food Science and Formulation, Gembloux Agro-Bio Tech, Universite de Liege, Passage des Deportes 2, B-5030 Gembloux, Belgium. LA - eng GR - S2026KFKT-09/Open Fund of Key Laboratory of Agro-Products Processing and Storage, Ministry of Agriculture and Rural Affairs/ GR - Open Fund of Tianjin International Joint Research and Development Center for Food Science and Engineering/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704128 OTO - NOTNLM OT - crystalline structure OT - freeze drying OT - microstructure OT - oven drying OT - retrogradation OT - starch gelatinization COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:36 PHST- 2026/09/20 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - foods15203752 [pii] AID - foods-15-03752 [pii] AID - 10.3390/foods15203752 [doi] PST - epublish SO - Foods. 2026 Oct 21;15(20):3752. doi: 10.3390/foods15203752. PMID- 42940390 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Thermal Processing of Watermeal for High-Protein Gluten-Free Rice Bread: Effects on Functional and Quality Properties. LID - 10.3390/foods15203755 [doi] LID - 3755 AB - Wolffia spp., commonly known as watermeal, is a nutrient-rich aquatic plant with potential as a sustainable food ingredient; however, its high moisture content (approximately 95%) necessitates effective drying to preserve product quality. This study evaluated hot-air oven and vacuum drying at 60 and 70 degrees C and subsequently investigated the effects of incorporating 0-20% watermeal powder into gluten-free rice bread as a product innovation. Vacuum drying at 70 degrees C produced powder with the lowest moisture content and water activity and the highest protein content (26.44 g/100 g), total phenolic content, and solubility. Based on these combined physicochemical and nutritional characteristics, the vacuum-drying-at-70 degrees C powder was selected for incorporation into gluten-free rice bread. The powder contained all nine essential amino acids, although their concentrations varied among drying treatments. Watermeal powder incorporation increased the viscoelastic moduli of the batter and enhanced its structural resistance and recovery behavior. Incorporating 20% watermeal powder increased the protein and crude fiber contents of the bread from 5.65 to 8.15 g/100 g and from 1.33 to 5.08 g/100 g, respectively, while decreasing the specific volume from 2.64 to 1.83 cm(3)/g and loaf height from 43.92 to 39.17 mm. Bread staling was evaluated during 3 days of storage at 25 +/- 2 degrees C. Despite these changes, the bread remained sensorially acceptable, with an overall-liking score of 6.0. These findings demonstrate the potential of dried watermeal as a nutrient-rich functional ingredient for gluten-free bread, providing nutritional enhancement while maintaining acceptable product quality. FAU - Juaming, Sunita AU - Juaming S AD - Division of Product Development Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. FAU - Clarica, Clarica AU - Clarica C AD - Division of Product Development Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. AD - Department of Food Technology, Indonesia International Institute for Life Sciences, Jl. Pulomas Barat Kav. 88, Jakarta 13210, Indonesia. FAU - Phimolsiripol, Yuthana AU - Phimolsiripol Y AUID- ORCID: 0000-0003-3521-1599 AD - Division of Product Development Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. FAU - Jadeja, Ravi AU - Jadeja R AUID- ORCID: 0000-0001-6964-817X AD - Robert M. Kerr Food & Agricultural Products Center, Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK 74078, USA. FAU - Tridtitanakiat, Pavalee Chompoorat AU - Tridtitanakiat PC AUID- ORCID: 0000-0002-4783-3435 AD - Division of Product Development Technology, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. AD - Lanna Rice Research Center, Chiang Mai University, Chiang Mai 50200, Thailand. AD - Sensory Evaluation and Consumer Testing Unit, Faculty of Agro-Industry, Chiang Mai University, Chiang Mai 50100, Thailand. LA - eng GR - Chiang Mai University/ GR - Oklahoma State University/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704453 OTO - NOTNLM OT - functional nutrition OT - gluten-free product development OT - thermal processing OT - watermeal powder COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:36 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - foods15203755 [pii] AID - foods-15-03755 [pii] AID - 10.3390/foods15203755 [doi] PST - epublish SO - Foods. 2026 Oct 21;15(20):3755. doi: 10.3390/foods15203755. PMID- 42940385 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Effects of Steam Explosion Combined with Ultrafine Grinding on the Physicochemical Properties, Antioxidant Capacity, and Volatile Profiles of Morchella sextelata Stipe Powders. LID - 10.3390/foods15203748 [doi] LID - 3748 AB - Morchella sextelata stipe is an underutilized by-product with potential for value-added food applications. This study investigated whether steam explosion (SE) pretreatment could enhance the effects of subsequent ultrafine grinding (UG) on the physicochemical properties, antioxidant capacity, and volatile profiles of stipe powders. Conventional grinding, UG, SE, and combined SE-UG treatments were compared using particle-size analysis, SEM, FT-IR, XRD, antioxidant assays, and HS-SPME-GC-MS. SE-UG reduced the median particle size (D50) from 88.88 to 10.46 mum and increased the water solubility index from 31.20% to 36.55%. It also caused extensive microstructural disruption and altered crystallinity without major changes in functional groups. SE-UG showed the highest DPPH and ABTS radical-scavenging activities, whereas SE and SE-UG exhibited similarly high total antioxidant capacity, with no significant difference between the two treatments. Volatile profiling showed treatment-dependent changes, with several furans and pyrazines occurring at higher relative abundances in SE-containing groups. These findings indicate that SE pretreatment facilitates subsequent UG and improves selected physicochemical and antioxidant properties while markedly modifying the volatile profile, supporting the value-added utilization of M. sextelata stipes. FAU - Jin, Jiaqi AU - Jin J AD - Shandong Key Laboratory of Applied Technology for Protein and Peptide Drugs, School of Pharmaceutical Science and Food Engineering, Liaocheng University, Liaocheng 252000, China. AD - College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China. FAU - Lao, Fei AU - Lao F AUID- ORCID: 0000-0002-9670-1981 AD - College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China. FAU - Kong, Feng AU - Kong F AUID- ORCID: 0000-0003-3963-1072 AD - Shandong Key Laboratory of Applied Technology for Protein and Peptide Drugs, School of Pharmaceutical Science and Food Engineering, Liaocheng University, Liaocheng 252000, China. FAU - Cao, Lele AU - Cao L AD - Shandong Key Laboratory of Applied Technology for Protein and Peptide Drugs, School of Pharmaceutical Science and Food Engineering, Liaocheng University, Liaocheng 252000, China. FAU - Feng, Linhui AU - Feng L AD - Shandong Key Laboratory of Applied Technology for Protein and Peptide Drugs, School of Pharmaceutical Science and Food Engineering, Liaocheng University, Liaocheng 252000, China. FAU - Chen, Jia AU - Chen J AUID- ORCID: 0009-0007-8721-507X AD - Shandong Key Laboratory of Applied Technology for Protein and Peptide Drugs, School of Pharmaceutical Science and Food Engineering, Liaocheng University, Liaocheng 252000, China. FAU - Wu, Jihong AU - Wu J AD - College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China. FAU - Guo, Xingfeng AU - Guo X AUID- ORCID: 0009-0004-3811-2646 AD - Shandong Key Laboratory of Applied Technology for Protein and Peptide Drugs, School of Pharmaceutical Science and Food Engineering, Liaocheng University, Liaocheng 252000, China. AD - College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China. LA - eng GR - SDAIT-07-17/shandong Agricultural Industry Technology System/ GR - 2025KJH169/Higher Educational Youth Innovation Science and Technology Program of Shandong Province/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704706 OTO - NOTNLM OT - HS-SPME-GC-MS OT - mushroom by-products OT - particle size reduction OT - steam explosion OT - volatile compounds COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:36 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - foods15203748 [pii] AID - foods-15-03748 [pii] AID - 10.3390/foods15203748 [doi] PST - epublish SO - Foods. 2026 Oct 21;15(20):3748. doi: 10.3390/foods15203748. PMID- 42940379 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Live and Heat-Treated Lacticaseibacillus paracasei NB23 Improve Selected Functional and Physiological Outcomes in Naturally Aged Mice. LID - 10.3390/foods15203742 [doi] LID - 3742 AB - Live and heat-treated microbial preparations are candidate nutritional ingredients for supporting function during aging. This study evaluated Lacticaseibacillus paracasei NB23 in naturally aged male C57BL/6NCrl mice. Ten young mice served as a reference group, and forty aged mice received vehicle, live NB23 at 2.05 x 10(9) or 6.15 x 10(9) colony-forming units (CFU)/kg/day, or heat-treated NB23 at 6.15 x 10(9) cell equivalents/kg/day for 12 weeks. Relative to aged controls, NB23-treated mice had shorter water-maze acquisition latency, longer treadmill endurance, higher hepatic antioxidant enzyme activities and glycogen, and lower selected whole-brain oxidative-damage markers. High-dose live and heat-treated NB23 also increased grip strength and muscle glycogen. Gastrocnemius myofiber area and selected femoral microarchitectural outcomes improved, whereas rotarod performance and tibialis anterior myofiber area did not. Exploratory analyses of brain and intestinal transcripts, fecal metabolites, and microbiota-host associations did not establish a gut-mediated mechanism. Among 325 taxon-outcome pairs in the exploratory microbiome-host screen, 49 met nominal p < 0.05, but none survived Benjamini-Hochberg correction (minimum adjusted p = 0.08967). Assay-specific subsets comprised four mice per group for micro-CT and five per group for qPCR, fecal metabolites, and microbiome sequencing, with two gene-specific qPCR exclusions. These findings support further evaluation of NB23 as a functional food ingredient, while the small, male-only animal study does not establish clinical anti-aging efficacy or equivalence of the live and heat-treated preparations. FAU - Lee, Mon-Chien AU - Lee MC AD - Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan City 333325, Taiwan. AD - Center for General Education, Taipei Medical University, Taipei 110301, Taiwan. FAU - Lin, Ping-Jui AU - Lin PJ AD - New Bellus Enterprise Co., Ltd., Tainan 720008, Taiwan. FAU - Lin, Ting-Chun AU - Lin TC AD - New Bellus Enterprise Co., Ltd., Tainan 720008, Taiwan. FAU - Chou, Chia-Hsuan AU - Chou CH AD - New Bellus Enterprise Co., Ltd., Tainan 720008, Taiwan. FAU - Hsiao, Chien-Yu AU - Hsiao CY AUID- ORCID: 0000-0003-3301-8808 AD - Department of Nutrition and Health Sciences, Chang Gung University of Science and Technology, Kweishan, Taoyuan City 33303, Taiwan. FAU - Huang, Chi-Chang AU - Huang CC AUID- ORCID: 0000-0003-1446-6787 AD - Graduate Institute of Sports Science, National Taiwan Sport University, Taoyuan City 333325, Taiwan. LA - eng GR - NTSU No. 1141004/University-Industry Cooperation Fund/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704608 OTO - NOTNLM OT - Lacticaseibacillus paracasei NB23 OT - exploratory microbiome associations OT - functional food ingredient OT - heat-treated bacteria OT - naturally aged mice OT - physical performance OT - spatial learning COIS- P.-J.L., T.-C.L. and C.-H.C. are affiliated with New Bellus Enterprise Co., Ltd. The authors disclose their institutional and commercial affiliations in relation to the investigated NB23 preparation. All remaining authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:36 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - foods15203742 [pii] AID - foods-15-03742 [pii] AID - 10.3390/foods15203742 [doi] PST - epublish SO - Foods. 2026 Oct 20;15(20):3742. doi: 10.3390/foods15203742. PMID- 42940378 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Integrated Chemical Profiling and Toxicological Assessment of Total Polyphenols from Cydonia oblonga Mill. Reveals Potential Safety Concerns Under High-Dose Exposure. LID - 10.3390/foods15203737 [doi] LID - 3737 AB - The increasing consumption of polyphenol-enriched functional foods and dietary supplements highlights the importance of systematic safety evaluation of these bioactive ingredients under high-dose exposure conditions. This study comprehensively evaluated the toxicological responses associated with total polyphenols from Cydonia oblonga Mill. (TPCOM) and explored their potential biological mechanisms. Ultra-high-performance liquid chromatography coupled with quadrupole-Orbitrap mass spectrometry identified 89 selected phenolic and related constituents. Acute and 28-day repeated-dose oral toxicity studies were conducted with reference to OECD Guideline 423 and in accordance with OECD Guideline 407, respectively. High-dose TPCOM exposure resulted in dose-associated biochemical and histopathological alterations, with the liver and kidneys identified as major target organs. Integrated systems toxicology approaches, including network toxicology, molecular docking, molecular dynamics simulations, and untargeted plasma metabolomics, revealed potential molecular targets, metabolic disturbances, and candidate biomarkers associated with TPCOM exposure. These findings suggest that excessive intake of TPCOM may be associated with metabolic imbalance, stress-related signaling alterations, and organ injury responses. Further experimental validation is required to confirm the specific molecular pathways involved. This study provides a systematic framework for evaluating the safety of polyphenol-rich functional food ingredients under high-dose exposure conditions. FAU - Aikebaier, Alifeiye AU - Aikebaier A AD - Department of Pharmacology, School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China. AD - Xinjiang Key Laboratory of Natural Medicines Active Components and Drug Release Technology, Urumqi 830017, China. AD - Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices, Urumqi 830017, China. AD - Engineering Research Center of Xinjiang and Central Asian Medicine Resources, Ministry of Education, Urumqi 830017, China. FAU - Baishan, Alhar AU - Baishan A AD - Department of Pharmacology, School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China. AD - Xinjiang Key Laboratory of Natural Medicines Active Components and Drug Release Technology, Urumqi 830017, China. AD - Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices, Urumqi 830017, China. AD - Engineering Research Center of Xinjiang and Central Asian Medicine Resources, Ministry of Education, Urumqi 830017, China. FAU - Abudurousuli, Kayisaier AU - Abudurousuli K AD - School of Brain Science and Brain Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China. FAU - Dilimulati, Dilihuma AU - Dilimulati D AUID- ORCID: 0009-0004-9690-4350 AD - Department of Pharmacology, School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China. AD - Xinjiang Key Laboratory of Natural Medicines Active Components and Drug Release Technology, Urumqi 830017, China. AD - Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices, Urumqi 830017, China. AD - Engineering Research Center of Xinjiang and Central Asian Medicine Resources, Ministry of Education, Urumqi 830017, China. FAU - Paerhati, Yipaerguli AU - Paerhati Y AD - Department of Pharmacology, School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China. AD - Xinjiang Key Laboratory of Natural Medicines Active Components and Drug Release Technology, Urumqi 830017, China. AD - Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices, Urumqi 830017, China. AD - Engineering Research Center of Xinjiang and Central Asian Medicine Resources, Ministry of Education, Urumqi 830017, China. FAU - Maitikasimu, Kuerbannisha AU - Maitikasimu K AD - Xinjiang Institute of Materia Medica, Urumqi 830011, China. FAU - Wusiman, Yilixiati AU - Wusiman Y AUID- ORCID: 0009-0000-0416-596X AD - Department of Pharmacology, School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China. AD - Xinjiang Key Laboratory of Natural Medicines Active Components and Drug Release Technology, Urumqi 830017, China. AD - Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices, Urumqi 830017, China. AD - Engineering Research Center of Xinjiang and Central Asian Medicine Resources, Ministry of Education, Urumqi 830017, China. FAU - Yusufujiang, Nazhakaiti AU - Yusufujiang N AD - Department of Pharmacology, School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China. AD - Xinjiang Key Laboratory of Natural Medicines Active Components and Drug Release Technology, Urumqi 830017, China. AD - Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices, Urumqi 830017, China. AD - Engineering Research Center of Xinjiang and Central Asian Medicine Resources, Ministry of Education, Urumqi 830017, China. FAU - Qiu, Xiaoxiao AU - Qiu X AD - Department of Pharmacology, School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China. AD - Xinjiang Key Laboratory of Natural Medicines Active Components and Drug Release Technology, Urumqi 830017, China. AD - Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices, Urumqi 830017, China. AD - Engineering Research Center of Xinjiang and Central Asian Medicine Resources, Ministry of Education, Urumqi 830017, China. FAU - Zhou, Wenting AU - Zhou W AUID- ORCID: 0000-0003-2610-7634 AD - Department of Pharmacology, School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China. AD - Xinjiang Key Laboratory of Natural Medicines Active Components and Drug Release Technology, Urumqi 830017, China. AD - Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices, Urumqi 830017, China. AD - Engineering Research Center of Xinjiang and Central Asian Medicine Resources, Ministry of Education, Urumqi 830017, China. LA - eng GR - 2022TSYCCX0035/Tianshan Talents-Youth Science and Technology InnovationTalents Training Program of Xinjiang Autonomous Region/ GR - 81660696/Natural Science Foundation for Distinguished Young Scholars of Xinjiang Autonomous Region/ GR - 2021/Fourteenth Five-Year Plan" Key Discipline Construction Project of Xinjiang Autonomous Region/ GR - XJDX1713/Xinjiang Key Laboratory of Natural Medicines Active Components and Drug Release Technology/ GR - 2023/Xinjiang Key Laboratory of Biopharmaceuticals and Medical Devices/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704314 OTO - NOTNLM OT - Cydonia oblonga OT - functional food safety OT - metabolic markers OT - metabolomics OT - network toxicology OT - polyphenols OT - toxicological assessment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:36 PHST- 2026/08/20 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - foods15203737 [pii] AID - foods-15-03737 [pii] AID - 10.3390/foods15203737 [doi] PST - epublish SO - Foods. 2026 Oct 20;15(20):3737. doi: 10.3390/foods15203737. PMID- 42940376 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Screening of Metabolite Biomarkers for Antioxidant and Glycosidase Inhibitory Activities from Oat Resources. LID - 10.3390/foods15203740 [doi] LID - 3740 AB - Oat accessions vary substantially in their bioactive components and associated functional properties, but the metabolites underlying differences in the bioactivities of digested oat samples remain insufficiently characterized. In this study, samples obtained after simulated gastrointestinal digestion of 43 oat accessions were evaluated for 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging and alpha-glucosidase inhibitory activities, and their metabolite profiles were characterized using ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry. Eleven differential metabolites were detected between the groups with high and low DPPH radical-scavenging activity, while nine were detected between groups with contrasting alpha-glucosidase inhibitory activities. Random forest-based feature selection identified tricin 7-O-beta-D-glucoside, myricetin 3-O-glucoside, quercetin 3-O-galactoside, and beta-sitosterol as candidate markers associated with variation in DPPH radical-scavenging activity. The association of beta-sitosterol with the high-DPPH phenotype was interpreted as statistical rather than as evidence of direct radical-scavenging activity. Myricetin 3-O-glucoside, quercetin 3-O-galactoside, and reduced glutathione were identified as metabolic markers associated with alpha-glucosidase inhibition. The results revealed substantial variation in the in vitro functional properties and metabolite profiles of digested oat samples. These metabolic markers provide a new research basis for the efficient development and utilization of functional products. FAU - Zhou, Haitao AU - Zhou H AD - College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China. AD - Zhangjiakou Academy of Agricultural Sciences, Zhangjiakou 075000, China. FAU - Yang, Huiyong AU - Yang H AD - College of Agronomy, Hebei Agricultural University, Baoding 071001, China. FAU - Zhang, Yajie AU - Zhang Y AD - Zhangjiakou Academy of Agricultural Sciences, Zhangjiakou 075000, China. FAU - Hou, Rui AU - Hou R AD - Zhangjiakou Academy of Agricultural Sciences, Zhangjiakou 075000, China. FAU - Zhu, Yingying AU - Zhu Y AD - College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China. FAU - Zou, Liang AU - Zou L AUID- ORCID: 0000-0002-3208-2819 AD - College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China. AD - Key Laboratory of Coarse Cereal Processing of Ministry of Agriculture and Rural Affairs, Chengdu University, Chengdu 610106, China. AD - Chengdu Agricultural College, Chengdu 611130, China. LA - eng GR - CARS-07-B-3/China Agriculture Research System/ GR - 21326305D/Hebei Seed Science and Technology Innovation Team of Coarse Cereals and Food Legumes/ GR - HBCT2024070205/Hebei Agriculture Research System/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704744 OTO - NOTNLM OT - DPPH scavenging activity OT - metabolic biomarker OT - oat OT - alpha-Glucosidase inhibition COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:36 PHST- 2026/08/19 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - foods15203740 [pii] AID - foods-15-03740 [pii] AID - 10.3390/foods15203740 [doi] PST - epublish SO - Foods. 2026 Oct 20;15(20):3740. doi: 10.3390/foods15203740. PMID- 42940375 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Carcass Traits, Meat Quality, Hydrolyzed Amino Acid Composition, and GC-IMS Volatile Signal Profiles in Kazakh Lambs Fed Diets Containing Fermented Cottonseed Hulls. LID - 10.3390/foods15203739 [doi] LID - 3739 AB - Cottonseed hulls, a fibrous by-product of cotton processing, are a potentially valuable unconventional feed resource for ruminants, and microbial fermentation may improve their feeding value. This study preliminarily compared plasma antioxidant status, slaughter performance, meat quality, hydrolyzed amino acids, and tentatively assigned GC-IMS volatile signals among Kazakh lambs fed diets containing wheat straw (WS), cottonseed hulls (CSH), or fermented cottonseed hulls (FCSH). Thirty male lambs were allocated to three groups (10 per group), with one pen per diet; six lambs per group were selected for slaughter. At the animal level, plasma biochemical indices showed no nominal between-group differences, whereas T-SOD activity was nominally higher in CSH and FCSH than in WS. Among slaughter-selected lambs, the FCSH group had nominally higher pre-slaughter live weight and carcass weight than the WS and CSH groups, with no detectable differences in dressing percentage or conventional meat-quality traits. Muscle crude fat was nominally higher in the FCSH group than in the WS group, but not different from the CSH group. TAA, EAA, and EAA/TAA showed no nominal between-group differences. Four individual hydrolyzed amino acids and 11 of 66 GC-IMS signals had unadjusted p < 0.05, but none reached q < 0.05 after Benjamini-Hochberg FDR correction. Because each diet was represented by only one pen, treatment and pen effects cannot be separated, and these animal-level findings should be considered exploratory and require validation with independent pen replication. FAU - Wang, Caidie AU - Wang C AD - Xinjiang Herbivore Nutrition Laboratory for Meat & Milk, College of Animal Sciences, Xinjiang Agricultural University, Urumqi 830052, China. FAU - Yang, Honghai AU - Yang H AD - Xinjiang Herbivore Nutrition Laboratory for Meat & Milk, College of Animal Sciences, Xinjiang Agricultural University, Urumqi 830052, China. FAU - Zhou, Xiaoyan AU - Zhou X AUID- ORCID: 0009-0002-2078-7464 AD - Xinjiang Herbivore Nutrition Laboratory for Meat & Milk, College of Animal Sciences, Xinjiang Agricultural University, Urumqi 830052, China. FAU - Yishake, Hanzuohere AU - Yishake H AD - Xinjiang Herbivore Nutrition Laboratory for Meat & Milk, College of Animal Sciences, Xinjiang Agricultural University, Urumqi 830052, China. FAU - Li, Songcheng AU - Li S AD - Xinjiang Herbivore Nutrition Laboratory for Meat & Milk, College of Animal Sciences, Xinjiang Agricultural University, Urumqi 830052, China. FAU - Liu, Yan AU - Liu Y AD - Xinjiang Herbivore Nutrition Laboratory for Meat & Milk, College of Animal Sciences, Xinjiang Agricultural University, Urumqi 830052, China. FAU - Li, Hongman AU - Li H AD - Xinjiang Herbivore Nutrition Laboratory for Meat & Milk, College of Animal Sciences, Xinjiang Agricultural University, Urumqi 830052, China. LA - eng GR - 277206/2021 Postdoctoral Project of Xinjiang Agricultural University/ GR - 2021YFD1600702-2/National Key Research and Development Program of China/ GR - DXSCX2026064/Undergraduate Innovation Project of Xinjiang Agricultural University/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704721 OTO - NOTNLM OT - GC-IMS OT - Kazakh lambs OT - candidate volatile signals OT - fermented cottonseed hulls OT - hydrolyzed amino acids OT - meat quality COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:36 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - foods15203739 [pii] AID - foods-15-03739 [pii] AID - 10.3390/foods15203739 [doi] PST - epublish SO - Foods. 2026 Oct 20;15(20):3739. doi: 10.3390/foods15203739. PMID- 42940374 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Application of Antioxidants from Agro-Industrial By-Products in Dairy Fat Systems: Oxidation Kinetics of Ghee and Active Packaging for Butter. LID - 10.3390/foods15203736 [doi] LID - 3736 AB - Apple, pear and grape pomaces are underexploited sources of phenolic compounds, potential natural alternatives to synthetic antioxidants. This study evaluated their cold-macerated (4 degrees C) extracts in ghee and in butter packaged in active chitosan films. Extracts were obtained under a 2(2) factorial design (water or absolute ethanol; 24 or 48 h). Ethanol yielded higher total phenolic content (TPC) and antioxidant capacity than water, particularly in grape pomace. The 48 h ethanolic extracts of apple (AP-E), pear (PP-E) and grape (GP-E) pomaces were selected for their higher TPC and antioxidant capacity; GP-E showed an IC(50) at least 94% lower than AP-E and PP-E in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. In ghee under accelerated storage (30, 45 and 60 degrees C, 35 days), extracts were compared to a synthetic Butylated Hydroxyanisole/Butylated hydroxytoluene (BHA/BHT) blend using oxidation kinetics and Arrhenius modeling. GP-E exhibited the lowest oxidation rate constants at all temperatures, significantly below BHA/BHT. PP-E did not differ significantly from the synthetic blend at 45 and 60 degrees C, and AP-E presented no significant effect at 60 degrees C. In butter wrapped in functionalized chitosan films (15 days storage at 21 and 30 degrees C), GP-E films produced the lowest oxidation values. PP-E films did not differ significantly from polyethylene control. These results suggest GP-E, and to a lesser extent PP-E, as a potential natural antioxidant for high-fat dairy matrices. FAU - Hernandez-Montesinos, Itzel Yoali AU - Hernandez-Montesinos IY AUID- ORCID: 0009-0001-3406-2694 AD - Instituto Politecnico Nacional, Centro de Investigacion en Biotecnologia Aplicada, Carretera Estatal Santa Ines Tecuexcomac-Tepetitla, km 1.5, Tepetitla de Lardizabal 90700, Tlaxcala, Mexico. FAU - Carreon-Delgado, David Fernando AU - Carreon-Delgado DF AUID- ORCID: 0009-0008-0682-3952 AD - Instituto Politecnico Nacional, Centro de Investigacion en Biotecnologia Aplicada, Carretera Estatal Santa Ines Tecuexcomac-Tepetitla, km 1.5, Tepetitla de Lardizabal 90700, Tlaxcala, Mexico. FAU - Castro-Lopez, Liliana Del Rocio AU - Castro-Lopez LDR AUID- ORCID: 0000-0002-2491-7433 AD - Facultad de Enologia y Gastronomia, Universidad Autonoma de Baja California, Carretera Transpeninsular Ensenada-Tijuana No. 3917, Colonia Playitas, Ensenada 22860, Baja California, Mexico. FAU - Ramirez-Lopez, Carolina AU - Ramirez-Lopez C AUID- ORCID: 0000-0002-9757-112X AD - Instituto Politecnico Nacional, Centro de Investigacion en Biotecnologia Aplicada, Carretera Estatal Santa Ines Tecuexcomac-Tepetitla, km 1.5, Tepetitla de Lardizabal 90700, Tlaxcala, Mexico. FAU - Lazo Zamalloa, Oxana AU - Lazo Zamalloa O AUID- ORCID: 0000-0002-8176-5907 AD - Instituto Politecnico Nacional, Centro de Investigacion en Biotecnologia Aplicada, Carretera Estatal Santa Ines Tecuexcomac-Tepetitla, km 1.5, Tepetitla de Lardizabal 90700, Tlaxcala, Mexico. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704126 OTO - NOTNLM OT - active packaging OT - agro-industrial by-products OT - ghee OT - oxidative stability OT - phenolic antioxidants OT - pomace extracts COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:36 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - foods15203736 [pii] AID - foods-15-03736 [pii] AID - 10.3390/foods15203736 [doi] PST - epublish SO - Foods. 2026 Oct 20;15(20):3736. doi: 10.3390/foods15203736. PMID- 42940371 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Occurrence of and Dietary Exposure to Toxic Element Impurities in Food Additives: A Probabilistic Exposure Assessment for the Belgian Population. LID - 10.3390/foods15203734 [doi] LID - 3734 AB - Toxic element impurities in food additives may contribute to the dietary exposure to arsenic (As), cadmium (Cd), lead (Pb), and mercury (Hg). This study aimed to quantify these toxic elements in selected food additives available on the Belgian market and to assess their contribution to dietary exposure and associated health risks. Seventeen food additives were analyzed using ICP-MS/MS, direct mercury analysis, and LC-ICP-MS. Dietary exposure to toxic elements originating from food additives was estimated for different age groups using probabilistic modeling based on occurrence data and EFSA intake estimates for the food additives. The highest element concentrations were measured in algae-based additives, notably sodium alginate and carrageenan. Arsenic speciation showed predominantly organic forms in sodium alginate, while carrageenan contained up to 32% inorganic arsenic. Mineral additives and pectin also contributed to toxic element occurrence, whereas additives produced via a purification process showed negligible levels. The exposure to Pb through the combined intake of multiple food additives may reach up to ~18% of total dietary Pb exposure in adults. For children, margins of exposure for Pb and inorganic As were low ( approximately 2-6), indicating that a health concern cannot be excluded. Exposure to Cd and Hg remained well below health-based guidance values. FAU - Vansnick, Celeste AU - Vansnick C AUID- ORCID: 0009-0006-2393-270X AD - Risk and Health Impact Assessment, Sciensano, 1050 Brussels, Belgium. AD - Trace Elements and Nanomaterials, Sciensano, 3080 Tervuren, Belgium. FAU - Cheyns, Karlien AU - Cheyns K AD - Trace Elements and Nanomaterials, Sciensano, 3080 Tervuren, Belgium. AD - Flemish Institute for Technological Research (VITO), 2400 Mol, Belgium. FAU - Montalvo, Daniela AU - Montalvo D AUID- ORCID: 0000-0002-6277-0583 AD - Trace Elements and Nanomaterials, Sciensano, 3080 Tervuren, Belgium. FAU - Waegeneers, Nadia AU - Waegeneers N AUID- ORCID: 0000-0001-6941-1287 AD - Risk and Health Impact Assessment, Sciensano, 1050 Brussels, Belgium. LA - eng GR - RF 23/23/FPS Public Health, Food Chain Safety and Environment/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704562 OTO - NOTNLM OT - Monte Carlo simulation OT - dietary exposure OT - food additives OT - impurities OT - toxic elements COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:36 PHST- 2026/09/09 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - foods15203734 [pii] AID - foods-15-03734 [pii] AID - 10.3390/foods15203734 [doi] PST - epublish SO - Foods. 2026 Oct 20;15(20):3734. doi: 10.3390/foods15203734. PMID- 42940360 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 19 TI - Pea Yogurt Alternatives: Aroma Modification by Starter Selection and Carbohydrate Addition. LID - 10.3390/foods15203723 [doi] LID - 3723 AB - Pea yogurt alternatives integrate the physiological advantages of lactic acid bacteria (LAB) fermentation and the nutritional properties of pea proteins, but their development is limited by the undesirable beany off-flavor of peas. This study aims to improve the aroma profile of LAB-fermented pea milk samples by applying different starter cultures and supplementing carbohydrates. A total of 140 volatile organic compounds (VOCs) were detected using comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GCxGC-TOFMS). Changing starter cultures yielded 26 critical differential VOCs, whereas glucose or lactose supplementation at different levels yielded 27 and 26 critical differential VOCs in fermentations with starters 465 and 061, respectively. The up-regulated critical differential VOCs corresponded to fermentation-generated and biotransformation-generated volatiles, whereas the down-regulated ones were mainly off-odor volatiles in pea milk. Their quantitative patterns were starter culture- and carbohydrate-dependent. Effects of starter selection and carbohydrate addition on sensory characteristics were assessed by summing the relative odor activity values (ROAVs). The creamy, sweet and pungent odors were markedly enhanced by starters used for plant-based yogurt, while glucose or lactose addition at levels of 3% or 5% (w/w) enhanced creamy odor and inhibited the green odor. These findings provide useful strategies for aroma modification of pea yogurt alternatives. FAU - Yu, Gaiwen AU - Yu G AD - College of Agriculture and Biotechnology, Lishui University, Lishui 323000, China. FAU - Zhang, Caimeng AU - Zhang C AD - State Key Laboratory of Food Science and Resources, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. AD - School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. FAU - Li, Xingfei AU - Li X AD - State Key Laboratory of Food Science and Resources, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. AD - School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. FAU - Kong, Xiangzhen AU - Kong X AD - State Key Laboratory of Food Science and Resources, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. AD - School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. FAU - Chen, Yeming AU - Chen Y AD - State Key Laboratory of Food Science and Resources, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. AD - School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. FAU - Hua, Yufei AU - Hua Y AD - State Key Laboratory of Food Science and Resources, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. AD - School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China. LA - eng GR - 32272243 and 32072164/National Natural Science Foundation of China/ GR - 2022CXGC010603/Department of Science and Technology of Shandong Province/ GR - 2020M671342/China Postdoctoral Science Foundation/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704146 OTO - NOTNLM OT - GCxGC-TOFMS OT - carbohydrate supplementation OT - pea yogurt alternatives OT - sensory characteristics OT - starter cultures OT - volatile organic compounds COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:36 PHST- 2026/09/13 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - foods15203723 [pii] AID - foods-15-03723 [pii] AID - 10.3390/foods15203723 [doi] PST - epublish SO - Foods. 2026 Oct 19;15(20):3723. doi: 10.3390/foods15203723. PMID- 42940358 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 19 TI - Biosafety and Fermentation Performance of Wickerhamomyces anomalus Starter Cultures in Fully Washed and Modified Whole-Cherry Fermentation of Coffea canephora. LID - 10.3390/foods15203721 [doi] LID - 3721 AB - Fermentation strongly influences coffee quality, yet spontaneous fermentation often results in inconsistent sensory profiles due to uncontrolled microbial dynamics. This study evaluated the biosafety and fermentation performance of two Wickerhamomyces anomalus starter cultures, YWP1-3 and UV22-2, for controlled Robusta coffee fermentation. Biosafety was assessed based on antifungal susceptibility, hemolytic activity, and cytotoxicity toward Caco-2 cells. The starters were subsequently applied at different inoculum levels (OD(600) 15-45) for 24-72 h using fully washed and modified whole-cherry processes. Both strains exhibited gamma-hemolysis, but Caco-2 cell viability remained relatively high at low-to-moderate concentrations (/= 1 and were selected as candidate aroma-active compounds for further sensory evaluation. Aroma recombination and omission experiments provided further sensory evidence for the relevance of 13 aroma compounds, including ethyl furfuryl ether, 1-propanol, ethyl isovalerate, and acetoin, to the aroma profiles of representative production rounds. Quantitative analysis revealed marked round-dependent variations in higher alcohols, esters, and acids, highlighting dynamic changes in the volatile composition of Sauce-flavor base baijiu across successive production rounds. This work provides a quantitative and sensory basis for understanding aroma variation across production rounds within the investigated Sauce-flavor baijiu production system and may provide a reference for subsequent flavor evaluation and process optimization. FAU - Wang, Na AU - Wang N AD - China National Research Institute of Food and Fermentation Industries Co., Ltd., Beijing 100015, China. AD - College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China. FAU - Shen, Yi AU - Shen Y AD - Langjiu Distillery Co., Ltd., Luzhou 646500, China. FAU - Luo, Sen AU - Luo S AD - China National Research Institute of Food and Fermentation Industries Co., Ltd., Beijing 100015, China. AD - College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China. FAU - Cheng, Wei AU - Cheng W AD - Langjiu Distillery Co., Ltd., Luzhou 646500, China. FAU - Wang, Jing AU - Wang J AD - China National Research Institute of Food and Fermentation Industries Co., Ltd., Beijing 100015, China. FAU - Wang, Jian AU - Wang J AD - China National Research Institute of Food and Fermentation Industries Co., Ltd., Beijing 100015, China. FAU - Liu, Zixuan AU - Liu Z AD - China National Research Institute of Food and Fermentation Industries Co., Ltd., Beijing 100015, China. FAU - Gao, Hongbo AU - Gao H AD - China National Research Institute of Food and Fermentation Industries Co., Ltd., Beijing 100015, China. FAU - Liu, Bing AU - Liu B AD - College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China. FAU - Niu, Jiao AU - Niu J AUID- ORCID: 0000-0002-3756-9626 AD - Langjiu Distillery Co., Ltd., Luzhou 646500, China. LA - eng GR - 2022YFF0606800/National Key Research and Development Program of China/ GR - 2022YFF0606803/National Key Research and Development Program of China/ GR - 2025KYCXZ30/China Graduate Student Independent Innovation Research Project/ PT - Journal Article DEP - 20261017 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704351 OTO - NOTNLM OT - GC-MS/MS OT - Sauce-flavor baijiu OT - flavoromics OT - key aroma compounds OT - molecular sensory science COIS- Authors Na Wang, Sen Luo, Jing Wang, Jian Wang, Zixuan Liu, and Hongbo Gao were affiliated with China National Research Institute of Food and Fermentation Industries Co., Ltd. Authors Yi Shen, Wei Cheng, and Jiao Niu were affiliated with Langjiu Distillery Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:36 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - foods15203698 [pii] AID - foods-15-03698 [pii] AID - 10.3390/foods15203698 [doi] PST - epublish SO - Foods. 2026 Oct 17;15(20):3698. doi: 10.3390/foods15203698. PMID- 42940332 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 17 TI - Selenium Biofortification Enhances Functional Bioactive Components and Nutritional Quality of Edible Mung Bean Sprouts During Germination. LID - 10.3390/foods15203695 [doi] LID - 3695 AB - This work aimed to identify the optimal edible germination period for mung bean sprouts and investigate how selenium biofortification modulates gamma-aminobutyric acid (GABA), phenolic compounds, alpha-ketoglutarate (AKG), carotenoids, and vitamin C (VC), with a focus on improving their nutritional and functional quality as fresh edible vegetables. The results demonstrated that germination promoted the accumulation of GABA, total phenolics, total flavonoids, AKG, total carotenoids, and VC in sprouts. Nevertheless, excessively prolonged germination significantly decreased GABA, AKG, and VC contents (p < 0.05). Meanwhile, excessive lateral root outgrowth and full elongation of sprouts deteriorated both their functional bioactive profile and sensory edible quality. Accordingly, the 3-day germination stage was selected as the optimal harvest point for edible mung bean sprouts. Treatment with an appropriate concentration of Na(2)SeO(3) further enhanced the contents of GABA, AKG, VC, and total carotenoids. In addition, selenium treatment significantly increased (p < 0.05) free total phenolic content (TPC) and total flavonoid content (TFC) while markedly reducing (p < 0.05) bound TPC and TFC across all tested germination stages. UPLC-ESI-MS/MS analysis revealed vitexin and isovitexin as the predominant phenolic compounds in germinated mung beans, detected in both free and bound polyphenol fractions. During germination, free vitexin and isovitexin declined, whereas bound forms of these two compounds increased. Compared with untreated controls, Na(2)SeO(3) biofortification elevated free vitexin and isovitexin and lowered their bound fractions at different germination times. Collectively, these findings provide valuable theoretical foundations to guide the production of selenium-biofortified mung bean sprout products enriched with health-promoting bioactive ingredients for functional food applications. FAU - He, Xiangxiang AU - He X AUID- ORCID: 0000-0003-4488-2474 AD - College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China. AD - Henan International Joint Laboratory of Food Green Processing and Safety Control, Henan University of Science and Technology, Luoyang 471023, China. FAU - Yu, Shibin AU - Yu S AD - College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China. AD - Henan International Joint Laboratory of Food Green Processing and Safety Control, Henan University of Science and Technology, Luoyang 471023, China. FAU - Zhang, Bo AU - Zhang B AUID- ORCID: 0000-0002-5011-1505 AD - School of Basic Medical Sciences, Zunyi Medical University, Zunyi 563000, China. AD - Zunyi Center for Disease Control and Prevention (Zunyi Health Supervision Bureau), Zunyi 563000, China. FAU - Yang, Runqiang AU - Yang R AUID- ORCID: 0000-0002-0191-6315 AD - College of Food Science & Technology, Nanjing Agricultural University, Nanjing 210095, China. FAU - Han, Yizhuo AU - Han Y AD - College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China. FAU - Hao, Hongwei AU - Hao H AD - College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China. FAU - Xiang, Jinle AU - Xiang J AUID- ORCID: 0000-0001-5652-117X AD - College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, China. AD - Henan International Joint Laboratory of Food Green Processing and Safety Control, Henan University of Science and Technology, Luoyang 471023, China. LA - eng GR - 32502094/National Natural Science Foundation of China/ GR - 252300423568/Natural Science Foundation of Henan Province/ GR - 242102110102, 252102111066, 262102110132/Henan Provincial Science and Technology Research Project/ PT - Journal Article DEP - 20261017 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704497 OTO - NOTNLM OT - bioactive compounds OT - germination period OT - mung bean sprouts OT - secondary metabolites OT - selenium biofortification COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:36 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - foods15203695 [pii] AID - foods-15-03695 [pii] AID - 10.3390/foods15203695 [doi] PST - epublish SO - Foods. 2026 Oct 17;15(20):3695. doi: 10.3390/foods15203695. PMID- 42940329 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - Integrated Biorefineries for Food Waste and Food-Processing Side Streams Through Feedstock-Oriented Design and Sustainable Process Integration. LID - 10.3390/foods15203692 [doi] LID - 3692 AB - Food waste represents a renewable carbon resource with considerable potential for the production of bioenergy, platform chemicals, biomaterials, and other high-value products within integrated biorefinery systems. However, the heterogeneous composition of food waste, together with the diversity of available conversion technologies, complicates the selection of efficient and economically viable valorization strategies. Successful food-waste valorization requires a systems-oriented approach that integrates individual technologies with feedstock characteristics, pretreatment, conversion pathways, and resource recovery into coherent biorefinery configurations. This structured critical review synthesizes literature identified through Scopus, Web of Science Core Collection, and ScienceDirect for the period January 2015-September 2026, and critically examines the evolution of food-waste biorefinery concepts from conventional processing to integrated, cascade, circular, and near-zero-waste systems, highlighting their engineering principles, advantages, and limitations. Current pretreatment technologies and biological, chemical and thermochemical conversion pathways are comparatively evaluated with respect to process performance, technology readiness and industrial applicability. Particular attention is given to integrated biorefinery design, emphasizing feedstock-oriented process selection, cascade valorization, process integration and resource recovery. The complementary roles of life-cycle assessment, techno-economic analysis, circularity metrics and technology readiness are discussed as key tools for supporting decision-making and industrial implementation. Future progress will depend on combining mature conversion technologies with digitalization, adaptive process management and integrated system design to maximize resource efficiency while advancing the transition toward a sustainable circular bioeconomy. FAU - Lazar Cosbuc, Ersilia Daniela AU - Lazar Cosbuc ED AD - Department of Environmental Engineering and Management, "Cristofor Simionescu" Faculty of Chemical Engineering and Environmental Protection, "Gheorghe Asachi" Technical University of Iasi, 73 Mangeron Blvd., 700050 Iasi, Romania. FAU - Oprea Blanaru, Paula Sanziana AU - Oprea Blanaru PS AD - Department of Environmental Engineering and Management, "Cristofor Simionescu" Faculty of Chemical Engineering and Environmental Protection, "Gheorghe Asachi" Technical University of Iasi, 73 Mangeron Blvd., 700050 Iasi, Romania. FAU - Ungureanu-Comanita, Elena-Diana AU - Ungureanu-Comanita ED AUID- ORCID: 0009-0006-9283-9568 AD - Department of Environmental Engineering and Management, "Cristofor Simionescu" Faculty of Chemical Engineering and Environmental Protection, "Gheorghe Asachi" Technical University of Iasi, 73 Mangeron Blvd., 700050 Iasi, Romania. FAU - Teodosiu, Carmen AU - Teodosiu C AUID- ORCID: 0009-0003-8058-6282 AD - Department of Environmental Engineering and Management, "Cristofor Simionescu" Faculty of Chemical Engineering and Environmental Protection, "Gheorghe Asachi" Technical University of Iasi, 73 Mangeron Blvd., 700050 Iasi, Romania. FAU - Gavrilescu, Maria AU - Gavrilescu M AUID- ORCID: 0000-0002-0663-0316 AD - Department of Environmental Engineering and Management, "Cristofor Simionescu" Faculty of Chemical Engineering and Environmental Protection, "Gheorghe Asachi" Technical University of Iasi, 73 Mangeron Blvd., 700050 Iasi, Romania. AD - Academy of Romanian Scientists, 3 Ilfov Street, 050094 Bucharest, Romania. AD - Academy of Technical Sciences of Romania, 26 Dacia Blvd., 030167 Bucharest, Romania. LA - eng GR - PN-IV-P6-6.1-CoEx-2024-0056, contract 6 CoEx/02.06.2026, within PNCDI IV/Ministry of Research, Innovation and Digitization, CNCS/CCCDI - UEFISCDI, Romania/ PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704576 OTO - NOTNLM OT - cascade valorization OT - circular bioeconomy OT - food waste OT - integrated biorefinery OT - process integration OT - technology readiness level COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:36 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - foods15203692 [pii] AID - foods-15-03692 [pii] AID - 10.3390/foods15203692 [doi] PST - epublish SO - Foods. 2026 Oct 16;15(20):3692. doi: 10.3390/foods15203692. PMID- 42940328 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - Effects of Brewing Temperature and Time on the Quality Attributes and Chemical Profiles of Cyclocarya paliurus Tea Infusions. LID - 10.3390/foods15203691 [doi] LID - 3691 AB - Brewing temperature and duration jointly modulate the sensory perception, functional properties, and chemical composition of herbal tea infusions, yet their integrated effects on Cyclocarya paliurus tea remain poorly understood. Here, cold-brewed (4 degrees C) and hot-brewed (90 degrees C) C. paliurus infusions prepared over a 5 min-2 h time gradient were systematically characterized using a multi-platform analytical strategy. Brewing mode and time produced clearly differentiated quality profiles: hot brewing yielded darker infusions with stronger bitterness and astringency, higher phenolic and flavonoid contents, and greater 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical-scavenging capacities, whereas cold brewing produced lighter, milder infusions with more gradual time-dependent compositional changes. Volatile and non-volatile profiles were clearly separated between the two brewing modes, and their temporal patterns indicated compound-specific temperature- and time-dependent responses rather than a generalized increase in extraction efficiency at elevated temperature. In total, 61 volatile features and 3666 metabolite features were annotated, from which 50 core differential metabolites were screened; exploratory treatment-level correlation analysis linked tentatively annotated gossypetin 8-glucuronide to radical-scavenging capacity, kaempferol 3-O-arabinoside and sericoside to bitterness-related responses, and epoxypheophorbide a to color variation. These findings clarify how brewing temperature and time jointly shape the quality of C. paliurus tea infusions and provide candidate chemical markers to guide the selection of brewing conditions and the development of differentiated C. paliurus-based herbal beverages. As a single commercial batch of C. paliurus tea was examined, these findings should be interpreted within the context of the tested material and brewing conditions. FAU - Fu, Yanmeng AU - Fu Y AUID- ORCID: 0009-0008-9669-1876 AD - TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Material Medical Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Zhao, Jingyi AU - Zhao J AD - TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Material Medical Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Zhou, Wenhui AU - Zhou W AD - TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Material Medical Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Ye, Jiaxing AU - Ye J AD - TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Material Medical Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Long, Bin AU - Long B AD - TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Material Medical Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Huang, Nan AU - Huang N AD - TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Material Medical Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Wang, Wei AU - Wang W AUID- ORCID: 0000-0003-0876-2205 AD - TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Material Medical Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Tian, Xing AU - Tian X AUID- ORCID: 0000-0002-1110-656X AD - TCM and Ethnomedicine Innovation & Development International Laboratory, Innovative Material Medical Research Institute, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. AD - Department of Food and Drug Engineering, School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. AD - Hunan Engineering and Technology Research Center for Health Products and Life Science, Changsha 410208, China. LA - eng GR - 2023JJ30445/Hunan Natural Science Foundation Program/ GR - B2024012/Hunan Province Traditional Chinese Medicine Research Project/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13703686 OTO - NOTNLM OT - Cyclocarya paliurus OT - cold brewing OT - hot brewing OT - radical-scavenging capacity OT - sensory quality OT - untargeted metabolomics OT - volatile compounds COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:36 PHST- 2026/09/30 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - foods15203691 [pii] AID - foods-15-03691 [pii] AID - 10.3390/foods15203691 [doi] PST - epublish SO - Foods. 2026 Oct 16;15(20):3691. doi: 10.3390/foods15203691. PMID- 42940327 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - Authentication and Quantitative Analysis of Adulteration in Ziziphi Spinosae Semen Based on Near-Infrared Spectroscopy and Chemometrics. LID - 10.3390/foods15203690 [doi] LID - 3690 AB - Ziziphi Spinosae Semen (ZSS) is a traditional Chinese medicinal material used for both medicinal and dietary purposes, but substitution and adulteration can compromise its authenticity and quality. Ten batches of authentic ZSS and one source batch of each of five adulterant materials were used to prepare 325 laboratory samples. This study aimed to develop a rapid method for ZSS identification and adulteration quantification using Fourier transform near-infrared spectroscopy (FT-NIR). Spectral distributions of six sample classes were analyzed using PCA-X, PLS-DA, and OPLS-DA, and the classification performance of SVM, DA, ANN, and KNN was compared. DD-SIMCA was used for one-class authentication, while PLSR was applied to quantify five adulterants using 15 spectral processing approaches. In the internal test set, SVM achieved 98.0% accuracy and 98.4% balanced accuracy. Within the laboratory samples studied, DD-SIMCA achieved 100% accuracy, sensitivity, and specificity. Across the five selected PLSR models, prediction-set R(2) ranged from 0.9272 to 0.9940 and RMSEP from 1.36 to 4.33 percentage points of adulterant mass fraction. These results demonstrate the feasibility of combining FT-NIR with chemometric methods for rapid ZSS classification, authenticity assessment, and adulteration-level prediction. Independent validation with additional source batches is required before practical application. FAU - Zhang, Yanyan AU - Zhang Y AUID- ORCID: 0009-0005-7738-1846 AD - School of Basic Chinese Medicine (School of Qihuang Medicine), Guizhou University of Traditional Chinese Medicine, Guizhou 550025, China. FAU - Pu, Xiang AU - Pu X AUID- ORCID: 0000-0003-3566-5363 AD - School of Basic Chinese Medicine (School of Qihuang Medicine), Guizhou University of Traditional Chinese Medicine, Guizhou 550025, China. FAU - Fan, Zhiliang AU - Fan Z AD - School of Basic Chinese Medicine (School of Qihuang Medicine), Guizhou University of Traditional Chinese Medicine, Guizhou 550025, China. FAU - Li, Yuexing AU - Li Y AD - School of Basic Chinese Medicine (School of Qihuang Medicine), Guizhou University of Traditional Chinese Medicine, Guizhou 550025, China. FAU - Chai, Yihui AU - Chai Y AD - School of Basic Chinese Medicine (School of Qihuang Medicine), Guizhou University of Traditional Chinese Medicine, Guizhou 550025, China. LA - eng GR - (No. Qiankehe Platform ZSYS [2025] 018)/Guizhou Key Laboratory of Miao Medicine/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13703979 OTO - NOTNLM OT - DD-SIMCA OT - Fourier transform spectroscopy OT - ZSS OT - authenticity identification OT - chemometrics OT - near-infrared spectroscopy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:36 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - foods15203690 [pii] AID - foods-15-03690 [pii] AID - 10.3390/foods15203690 [doi] PST - epublish SO - Foods. 2026 Oct 16;15(20):3690. doi: 10.3390/foods15203690. PMID- 42940315 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - The Widening Cross-Country Food Availability Gap. LID - 10.3390/foods15203678 [doi] LID - 3678 AB - National food supplies have improved almost everywhere since 2000, yet progress towards Zero Hunger has stalled. This study examines how the cross-country distribution of food availability evolved and what contributed to its renewed widening, using the FAO indicator of average dietary energy supply adequacy. For 150 countries observed from 2001 to 2023, dispersion declined rapidly through the late 2000s, remained relatively stable until the mid-2010s, and subsequently increased. A continuous three-segment model outperforms both a two-segment V and a constrained plateau model (DeltaAICc = 39.75 and 12.10, respectively) and is selected in 98.7% of three-year moving-block bootstrap samples. The three-phase pattern is also recovered from the latest FAOSTAT vintage, although historical revisions affect individual estimates. Most renewed widening occurs within geographic regions and subregions, while 93% of the 2013-2023 increase in the 90th-10th percentile gap originates below the median. Countries initially occupying the lowest adequacy quintile improved faster than those in the highest, but transition matrices provide no evidence of increasing mobility. Political stability and adequacy exhibit contemporaneous co-movement, although this association cannot be interpreted causally. Food-security monitoring should complement national and regional averages with distributional indicators that capture cross-country disparities. The concentration of renewed divergence within regions and in the lower half of the distribution suggests that food-security policies should target country-specific vulnerabilities rather than rely solely on broad regional classifications. In particular, tracking countries that persistently remain in or newly enter the lowest adequacy group could help prioritise support and better target food-security interventions. Overall, the findings highlight the importance of distribution-sensitive monitoring and policy approaches for advancing SDG 2 without assuming a universal turning year or a common trajectory across countries. FAU - Cati, Enes AU - Cati E AUID- ORCID: 0000-0002-2881-6891 AD - Department of Agricultural Economics, Faculty of Agriculture, Malatya Turgut Ozal University, 44210 Malatya, Turkiye. FAU - Gunduz, Orhan AU - Gunduz O AUID- ORCID: 0000-0002-2357-0802 AD - Department of Agricultural Economics, Faculty of Agriculture, Malatya Turgut Ozal University, 44210 Malatya, Turkiye. FAU - Gultekin, Ufuk AU - Gultekin U AUID- ORCID: 0000-0002-4378-8868 AD - Department of Agricultural Economics, Faculty of Agriculture, Cukurova University, 01250 Adana, Turkiye. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704660 OTO - NOTNLM OT - SDG 2 OT - cross-country divergence OT - dietary energy supply adequacy OT - food availability OT - food security monitoring OT - import dependency OT - political stability COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:36 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - foods15203678 [pii] AID - foods-15-03678 [pii] AID - 10.3390/foods15203678 [doi] PST - epublish SO - Foods. 2026 Oct 16;15(20):3678. doi: 10.3390/foods15203678. PMID- 42940299 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Verification of Official LC-MS/MS Methods and Nationwide Monitoring of Five Prohibited Veterinary Drugs and a Major Metabolite in Livestock Muscle in Korea. LID - 10.3390/foods15203662 [doi] LID - 3662 AB - This study evaluated the fitness for purpose of official Korean Food Code LC-MS/MS methods for five prohibited veterinary drugs and one major metabolite-pyrimethamine, dapsone, monoacetyldapsone, roxarsone, chloramphenicol, and vancomycin-in bovine, porcine, and chicken muscle and applied the evaluated methods to nationwide monitoring of production-stage samples. In 2024, dapsone, monoacetyldapsone, pyrimethamine, and roxarsone were analyzed in 202 muscle samples, whereas in 2025, chloramphenicol and vancomycin were analyzed in 300 muscle samples (total n = 502). Analytical performance was assessed for selectivity, linearity, limits of detection (LOD) and quantification (LOQ), recovery, repeatability, inter-day precision, and between-analyst performance; all analyte-matrix combinations met the predefined acceptance criteria. For chloramphenicol, an LOQ of 0.15 mug/kg was achieved in all three matrices, equal to the European Union reference point for action. An external proficiency test for chloramphenicol in bovine muscle yielded a satisfactory z-score of -0.30. No sample met the regulatory reporting criterion for any target analyte included in the respective annual survey. These findings support the fitness for purpose of the evaluated official methods and provide baseline analytical and occurrence data for surveillance of selected prohibited substances in production-stage meat in Korea. FAU - Park, Sunjin AU - Park S AUID- ORCID: 0000-0002-9553-5133 AD - Livestock Product Export and Sanitation Team, Animal and Plant Quarantine Agency (APQA), 177, Hyeoksin 8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, Republic of Korea. FAU - Kim, Se-Hyung AU - Kim SH AD - Livestock Product Export and Sanitation Team, Animal and Plant Quarantine Agency (APQA), 177, Hyeoksin 8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, Republic of Korea. FAU - Hong, Chung-Oui AU - Hong CO AD - Livestock Product Export and Sanitation Team, Animal and Plant Quarantine Agency (APQA), 177, Hyeoksin 8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, Republic of Korea. FAU - Kim, Do Hui AU - Kim DH AUID- ORCID: 0009-0003-4553-5703 AD - Livestock Product Export and Sanitation Team, Animal and Plant Quarantine Agency (APQA), 177, Hyeoksin 8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, Republic of Korea. FAU - Lee, Seon-Young AU - Lee SY AD - Livestock Product Export and Sanitation Team, Animal and Plant Quarantine Agency (APQA), 177, Hyeoksin 8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, Republic of Korea. FAU - Lee, Soo Kyoung AU - Lee SK AD - Department of Companion Animal Health Care, Kyung-In Woman's University, 63, Gyeyangsan-ro, Gyeyang-gu, Incheon 21041, Republic of Korea. FAU - Kim, Seok-Jae AU - Kim SJ AD - Livestock Product Export and Sanitation Team, Animal and Plant Quarantine Agency (APQA), 177, Hyeoksin 8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, Republic of Korea. FAU - Park, Mi-Young AU - Park MY AD - Livestock Product Export and Sanitation Team, Animal and Plant Quarantine Agency (APQA), 177, Hyeoksin 8-ro, Gimcheon-si 39660, Gyeongsangbuk-do, Republic of Korea. LA - eng GR - B-1543620-2026-28-01/Animal and Plant Quarantine Agency/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13703556 OTO - NOTNLM OT - LC-MS/MS OT - analytical performance verification OT - food safety OT - livestock muscle OT - nationwide monitoring OT - prohibited veterinary drugs COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:36 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - foods15203662 [pii] AID - foods-15-03662 [pii] AID - 10.3390/foods15203662 [doi] PST - epublish SO - Foods. 2026 Oct 15;15(20):3662. doi: 10.3390/foods15203662. PMID- 42940298 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - An Integrated Framework for Green Tea Infusion Chemical Characterization: Process Optimization, PCA-Based Chemometric Evaluation, and Untargeted Metabolomic Profiling. LID - 10.3390/foods15203661 [doi] LID - 3661 AB - This study investigated the optimization and quality evaluation of green tea infusions prepared from three commercial green tea samples from different production origins, namely Anhui Huangshan Maofeng (AH), Shaanxi Ziyang (SX), and Yunnan Xuelong (YN), using three extraction techniques: hot-water extraction (HWE), enzyme-assisted extraction (EAE), and cold brewing (CB). We used water-extractable content (WEC) as the sole response variable in a response surface methodology framework to optimize physical solid yield rather than chemical quality. We developed a principal component analysis (PCA) model that incorporates WEC with six essential functional constituents-epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG), epigallocatechin gallate (EGCG), caffeine, and L-theanine, to thoroughly evaluate infusion quality. Untargeted metabolomics was subsequently performed on three PCA-selected representative SX samples corresponding to PCA ranks 18, 58, and 107. Our findings demonstrated that WEC was significantly influenced by the investigated extraction variables, and validation of the BBD-RSM models showed absolute relative deviations of 0.12-0.81% between the predicted and experimentally measured WEC values. Six targeted components differed significantly among the three tea samples and extraction methods. The PCA score plots showed partial separation among the sample-process combinations, with the first two principal components explaining 78.45% of the total variance. Additionally, the three SX infusion groups were clearly distinguished by orthogonal partial least-squares discriminant analysis (OPLS-DA) in conjunction with KEGG pathway mapping; KEGG pathway mapping shows that the differentially enriched metabolites are primarily annotated to flavonoid, phenolic acid, amino acid derivative, and purine-related pathways. By combining RSM-based optimization, targeted HPLC measurement, PCA scoring, and non-targeted metabolomic profiling, this study created a strong framework for chemical evaluation. This integrated framework offers a valuable reference for selecting appropriate extraction processes and ensuring consistent quality in green tea infusions. FAU - Bai, Yi AU - Bai Y AD - School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China. AD - Ningxia Key Laboratory of Development and Utilization of Specialty Food Resources, Yinchuan 750021, China. FAU - Ma, Wen-Ping AU - Ma WP AD - School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China. AD - Ningxia Key Laboratory of Development and Utilization of Specialty Food Resources, Yinchuan 750021, China. FAU - Ni, Zhi-Jing AU - Ni ZJ AD - School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China. AD - Ningxia Key Laboratory of Development and Utilization of Specialty Food Resources, Yinchuan 750021, China. FAU - Wang, Wei AU - Wang W AD - School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China. AD - Ningxia Key Laboratory of Development and Utilization of Specialty Food Resources, Yinchuan 750021, China. FAU - Thakur, Kiran AU - Thakur K AD - School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China. AD - Ningxia Key Laboratory of Development and Utilization of Specialty Food Resources, Yinchuan 750021, China. FAU - Ma, Run-Hui AU - Ma RH AD - School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China. AD - Ningxia Key Laboratory of Development and Utilization of Specialty Food Resources, Yinchuan 750021, China. FAU - Wei, Zhao-Jun AU - Wei ZJ AUID- ORCID: 0000-0003-1729-209X AD - School of Biological Science and Engineering, North Minzu University, Yinchuan 750021, China. AD - Ningxia Key Laboratory of Development and Utilization of Specialty Food Resources, Yinchuan 750021, China. LA - eng GR - 2024BEE02023; 2025AAC030074; 2025CXTD006/Science and Technology Department of Ningxia/ GR - CYX25271/North Minzu University/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13703727 OTO - NOTNLM OT - PCA comprehensive evaluation OT - extraction optimization OT - green tea infusion OT - non-targeted metabolomics OT - target compounds COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:36 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - foods15203661 [pii] AID - foods-15-03661 [pii] AID - 10.3390/foods15203661 [doi] PST - epublish SO - Foods. 2026 Oct 15;15(20):3661. doi: 10.3390/foods15203661. PMID- 42940292 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Multi-Omics Integration Reveals Molecular Signatures Associated with Pork Quality Differences Between Liaodan Black and Xinjin Pigs. LID - 10.3390/foods15203656 [doi] LID - 3656 AB - This exploratory study investigated meat quality differences and associated molecular characteristics between Liaodan Black (LDM) and Xinjin (XJM) pigs, two local breeds from Northeast China, using meat quality, fatty acid, and total amino acid measurements together with transcriptomic, proteomic, and untargeted metabolomic analyses of the longissimus thoracis et lumborum muscle (n = 3 per breed for all analyses). XJM pigs had higher intramuscular fat content and lower shear force, whereas LDM pigs had higher cooking yield. After correction for multiple testing, XJM pigs had higher oleic acid content. Total amino acid analysis showed higher serine, arginine, proline, methionine, and phenylalanine contents in XJM pigs, whereas isoleucine and leucine contents were higher in LDM pigs. Transcriptomic and proteomic analyses identified 5678 differentially expressed genes and 533 differentially abundant proteins, respectively, while untargeted metabolomic analysis revealed 622 differential metabolites based on putative annotations. These molecular differences were mainly associated with cytoskeletal organization, oxidative phosphorylation, protein turnover, amino acid metabolism, and central carbon metabolism. Transcriptome-proteome integration identified 258 shared differential features, of which 139 showed concordant changes at the mRNA and protein levels. Integrated analysis identified nine candidate molecules. After Benjamini-Hochberg correction, exploratory correlation analysis showed that GOT1 and GLO1 protein abundances were negatively associated with shear force and cooking yield, whereas ESD protein abundance was positively associated with IMF content. qPCR analysis of selected DEGs showed good agreement with RNA-seq results (r = 0.883, p < 0.001). Overall, the differences observed between the sampled LDM and XJM groups were mainly associated with lipid deposition, muscle structure-related processes, and energy metabolism. FAU - Jia, Zhanpeng AU - Jia Z AUID- ORCID: 0009-0000-0899-2989 AD - College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. FAU - Tian, Yujinhe AU - Tian Y AD - College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. FAU - He, Yuqiao AU - He Y AD - College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. FAU - Liu, Xianjun AU - Liu X AUID- ORCID: 0000-0002-5790-6503 AD - College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. FAU - Qiao, Fubo AU - Qiao F AD - College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. FAU - Li, Wen AU - Li W AUID- ORCID: 0009-0009-8561-2308 AD - College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. FAU - Chen, Jing AU - Chen J AD - College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China. LA - eng GR - Grant No. 2023YFD1300200/National Key Research and Development Program of China/ GR - Grant No. 2024JH2/102400009/Key Research and Development Project of Liaoning Province/ GR - Grant No. 24-215-2-20/Shenyang Science and Technology Key Research Project/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13703621 OTO - NOTNLM OT - Liaodan black pig OT - Xinjin pig OT - meat quality OT - metabolomics OT - multi-omics OT - pork COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:36 PHST- 2026/09/20 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - foods15203656 [pii] AID - foods-15-03656 [pii] AID - 10.3390/foods15203656 [doi] PST - epublish SO - Foods. 2026 Oct 15;15(20):3656. doi: 10.3390/foods15203656. PMID- 42940289 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Sensory Characteristics and Consumer Acceptance of Commercial Yakgwa, a Traditional Korean Dessert, Among Vietnamese Consumers: A Check-All-That-Apply (CATA) Approach. LID - 10.3390/foods15203654 [doi] LID - 3654 AB - To explore the overseas potential of yakgwa, a traditional Korean honey confection, this study investigated the sensory characteristics and consumer acceptability of commercial yakgwa among Vietnamese consumers. Four products with distinct sensory characteristics were selected from 12 commercial products. A total of 104 Vietnamese consumers evaluated the samples using a check-all-that-apply (CATA) questionnaire comprising 39 sensory and image attributes, and rated acceptability, familiarity, willingness to eat again, and recommendation intention on 9-point scales. Data were analyzed using Cochran's Q test, analysis of variance, correspondence analysis, and multiple factor analysis (MFA). Samples 537 and 681 received significantly higher ratings than samples 214 and 825 for overall and most other acceptability ratings, familiarity, willingness to eat again, and recommendation intention. In the CATA evaluation, sample 214 was frequently described as oily-looking, crispy, and dry and rough, whereas sample 825 was associated with dark-colored, salty taste, herbal flavor, and cinnamon and ginger attributes. Samples 537 and 681 were more frequently described as light-colored, sweet-tasting, and soft. In the MFA, samples 537 and 681 were positioned closer to the acceptability and behavioral intention attributes than samples 214 and 825. Thus, light color, sweetness, and soft texture may be associated with higher acceptability, whereas oily appearance, dry and rough texture, and strong spice flavors may be associated with lower acceptability. However, this study was conducted in a single region and included many consumers unfamiliar with yakgwa; further research covering broader regions and age groups is needed. FAU - Lee, Juyoun AU - Lee J AUID- ORCID: 0009-0002-4400-5957 AD - Department of Food and Nutrition, Duksung Women's University, 33 Samyang-ro, Seoul 01369, Republic of Korea. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704176 OTO - NOTNLM OT - check-all-that-apply (CATA) OT - sensory evaluation OT - traditional Korean dessert OT - yakgwa COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:36 PHST- 2026/09/27 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - foods15203654 [pii] AID - foods-15-03654 [pii] AID - 10.3390/foods15203654 [doi] PST - epublish SO - Foods. 2026 Oct 15;15(20):3654. doi: 10.3390/foods15203654. PMID- 42940288 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - From Phosphorus Nutrition to Starch Functionality: Effects of Phosphorus Fertilization on Sorghum Starch Biosynthesis, Multiscale Structure, and Processing Properties. LID - 10.3390/foods15203651 [doi] LID - 3651 AB - Phosphorus fertilization can regulate starch accumulation and quality in cereal grains, but its effects on sorghum starch remain insufficiently understood. This study investigated sorghum cultivar Liaoza 82 under four phosphorus levels: 0, 40, 80, and 160 kg P(2)O(5) ha(-1). Phosphorus application significantly affected starch accumulation and the activities of starch biosynthesis-related enzymes, including AGPase, GBSS, SSS, and SBE. Moderate phosphorus, particularly 80 kg P(2)O(5) ha(-1), increased amylose content, granule size, molecular weight, amylopectin branching degree, and medium-to-long amylopectin chains, whereas the highest phosphorus treatment weakened these effects and caused granule surface depressions. Although the A-type crystalline pattern was unchanged, phosphorus modified crystallinity, short-range order, thermal properties, solubility, swelling power, and pasting behavior. Within the phosphorus range investigated, P2 produced a favorable combination of selected structural and thermal characteristics, including lower crystalline order and lower gelatinization temperature and enthalpy, whereas some pasting parameters were highest under P3. These findings indicate that phosphorus fertilization alters sorghum starch structure and functionality, with the response depending on the specific starch characteristic evaluated. FAU - Chen, Baizhi AU - Chen B AUID- ORCID: 0009-0003-0338-6121 AD - Sorghum Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China. FAU - Yang, Linlin AU - Yang L AD - Sorghum Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China. FAU - Ke, Fulai AU - Ke F AD - Sorghum Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China. FAU - Zhu, Kai AU - Zhu K AD - Sorghum Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China. LA - eng GR - 2026YJ1201/Emergency and Strategic Research Project of the Liaoning Academy of Agricultural Sciences/ GR - 2025BS1704/Doctoral Start-up Fund of the Liaoning Academy of Agricultural Sciences/ GR - 2025MD784217/General Program of the China Postdoctoral Science Foundation/ GR - CARS-06-14.5-A11/National Grain Sorghum Modern Agricultural Industrial Technology System for Forage Sorghum Breeding Post Scientist Project/ GR - 2025-BS-0928/Liaoning Provincial Doctoral Research Start-up Fund Program/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13703702 OTO - NOTNLM OT - gelatinization properties OT - phosphorus fertilization OT - sorghum starch OT - starch biosynthesis enzymes OT - starch structure COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:36 PHST- 2026/08/01 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - foods15203651 [pii] AID - foods-15-03651 [pii] AID - 10.3390/foods15203651 [doi] PST - epublish SO - Foods. 2026 Oct 15;15(20):3651. doi: 10.3390/foods15203651. PMID- 42940286 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Traditional Food Use and Ethnomedicinal Contexts of Turnip (Brassica rapa L.): A Scoping Review of Composition, Food Processing, and Health-Related Evidence. LID - 10.3390/foods15203648 [doi] LID - 3648 AB - Turnip (Brassica rapa L.) is widely cultivated for food and has documented ethnomedicinal uses. This scoping review mapped the international literature on turnip, with detailed synthesis focused on turnip materials associated with selected regional food and ethnomedicinal traditions in China. Searches covered seven bibliographic databases. The bibliographic map comprised 771 records, of which 397 were selected for detailed synthesis. Regional uses varied in plant part and preparation method. The clearest evidence concerned composition and processing: constituent profiles differed by provenance, plant part, and analytical method, whereas changes in composition and product quality depended on the product and process. Animal and cell studies reported effects of specific extracts, fractions, and multicomponent formulations on hypoxia tolerance, endurance, tissue injury, inflammation, redox regulation, metabolism, and the gut microbiota, but mechanistic findings did not converge across preparations and models. The few human studies addressed mainly hypoxia or exercise and supportive care during chemotherapy; differences in design, preparations, and outcomes precluded reproducible conclusions about any defined turnip food or ethnomedicinal preparation. Overall, this review clarifies the current evidence on turnip as a food resource, documents its ethnomedicinal contexts, and identifies priorities for future research and product development. FAU - Ye, Kanxin AU - Ye K AUID- ORCID: 0009-0008-5991-804X AD - Department of Development and Evaluation of Dietary Functional Products, Key Laboratory of Modern Preparation of Chinese Medicine, Ministry of Education, Jiangxi University of Chinese Medicine, Nanchang 330004, China. FAU - Zhang, Hua AU - Zhang H AUID- ORCID: 0000-0001-7100-8825 AD - Department of Development and Evaluation of Dietary Functional Products, Key Laboratory of Modern Preparation of Chinese Medicine, Ministry of Education, Jiangxi University of Chinese Medicine, Nanchang 330004, China. FAU - Hu, Huiqiang AU - Hu H AD - Department of Development and Evaluation of Dietary Functional Products, Key Laboratory of Modern Preparation of Chinese Medicine, Ministry of Education, Jiangxi University of Chinese Medicine, Nanchang 330004, China. FAU - Ye, Yaohui AU - Ye Y AD - Nanchang Medical College, Nanchang 330038, China. FAU - Chen, Lihua AU - Chen L AUID- ORCID: 0000-0002-6313-6496 AD - Department of Development and Evaluation of Dietary Functional Products, Key Laboratory of Modern Preparation of Chinese Medicine, Ministry of Education, Jiangxi University of Chinese Medicine, Nanchang 330004, China. FAU - Xiong, Yun AU - Xiong Y AUID- ORCID: 0000-0001-5941-0352 AD - Department of Development and Evaluation of Dietary Functional Products, Key Laboratory of Modern Preparation of Chinese Medicine, Ministry of Education, Jiangxi University of Chinese Medicine, Nanchang 330004, China. LA - eng GR - 2022294558/Science and Technology Department of Xinjiang Uygur Autonomous Region/ GR - 20212AAF01005/Department of Science and Technology of Jiangxi Province/ GR - BX20250218/China Postdoctoral Science Foundation/ PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13703587 OTO - NOTNLM OT - Brassica rapa OT - Qiamagu OT - bioactivity OT - ethnomedicine OT - food processing OT - phytochemicals OT - scoping review OT - turnip COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:36 PHST- 2026/08/27 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - foods15203648 [pii] AID - foods-15-03648 [pii] AID - 10.3390/foods15203648 [doi] PST - epublish SO - Foods. 2026 Oct 14;15(20):3648. doi: 10.3390/foods15203648. PMID- 42940284 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Development of Functional Lamb Pate Enriched with Edible Lamb By-Products and Pulse Ingredients: Physicochemical, Nutritional and Safety Evaluation. LID - 10.3390/foods15203647 [doi] LID - 3647 AB - The growing demand for nutritionally enriched and sustainable meat products has stimulated interest in the utilization of edible animal by-products and plant-based ingredients. The present study aimed to develop lamb pate formulations containing lamb liver, lamb heart, mung bean, and chickpea and to evaluate their physicochemical, functional-technological, nutritional, microbiological, and contaminant characteristics. Four formulations were prepared, including a control without lamb offal and three experimental formulations containing 13%, 15%, and 17% lamb offal. Mung bean and chickpea were maintained at fixed levels of 7.5% each in all formulations; therefore, the experimental design primarily evaluated the replacement of lean lamb meat with increasing proportions of liver and heart. The effects of lamb offal incorporation on proximate composition, water-holding capacity, water-retention capacity, sensory characteristics, amino acid composition, vitamin and mineral composition, microbiological quality, and heavy metal content were investigated. Among the formulations evaluated, O-2 containing 15% lamb offal showed a favorable combination of physicochemical, water-binding, and sensory characteristics, including a protein content of 26.0%, fat content of 24.9%, moisture content of 62.5%, and the highest water-holding capacity (70.0%). O-2 also received the highest numerical sensory scores, although these were not significantly different from O-1 for several sensory attributes. Amino acid analysis confirmed the presence of essential and non-essential amino acids in the selected O-2 formulation. Compared with the control, O-2 showed numerically higher concentrations of the investigated B-group vitamins and significantly higher concentrations of iron, calcium, and potassium. At the investigated time point, O-2 showed a low total viable count (2.1 x 10(2) CFU/g), no detectable pathogenic microorganisms, and compliance with the evaluated microbiological criteria; lead and mercury were not detected under the analytical conditions used. Overall, the results indicate that partial replacement of lean lamb meat with edible lamb liver and heart within the fixed pulse-containing formulation can provide pate with favorable physicochemical, water-binding, sensory, and nutritional characteristics. Further storage studies are required to establish shelf-life stability and microbiological safety throughout storage. FAU - Tokanova, Zhuldyz AU - Tokanova Z AD - Department of Food Technologies, Shakarim University, 20 A Glinka Street, Semey 071410, Kazakhstan. FAU - Amirkhanov, Kumarbek AU - Amirkhanov K AD - Department of Food Technologies, Shakarim University, 20 A Glinka Street, Semey 071410, Kazakhstan. FAU - Kassymov, Samat AU - Kassymov S AUID- ORCID: 0000-0001-7384-6996 AD - Department of Food Technologies, Shakarim University, 20 A Glinka Street, Semey 071410, Kazakhstan. FAU - Assirzhanova, Zhanna AU - Assirzhanova Z AD - Department of Food Technologies, Shakarim University, 20 A Glinka Street, Semey 071410, Kazakhstan. FAU - Iskakova, Zhanat AU - Iskakova Z AUID- ORCID: 0000-0002-2688-0670 AD - Department of Food Safety and Technology, Kazakh National Agrarian Research University, 8 Abay Avenue, Almaty 050010, Kazakhstan. FAU - Baikadamova, Assemgul AU - Baikadamova A AUID- ORCID: 0000-0003-0062-6997 AD - Department of Food Technologies, Shakarim University, 20 A Glinka Street, Semey 071410, Kazakhstan. FAU - Khaimuldinova, Altyngul AU - Khaimuldinova A AD - Department of Standardization, Certification and Metrology, L. N. Gumilyov Eurasian National University, 2 Satpayev Street, Astana 010008, Kazakhstan. FAU - Imanbayev, Alimzhan AU - Imanbayev A AD - Department of Technology and Food Safety, M. Auezov South Kazakhstan State University, 5 Tauke Khan Avenue, Shymkent 160012, Kazakhstan. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13703730 OTO - NOTNLM OT - chickpea OT - edible animal by-products OT - hybrid meat products OT - lamb heart OT - lamb liver OT - lamb pate OT - mung bean COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:36 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - foods15203647 [pii] AID - foods-15-03647 [pii] AID - 10.3390/foods15203647 [doi] PST - epublish SO - Foods. 2026 Oct 14;15(20):3647. doi: 10.3390/foods15203647. PMID- 42940276 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Interpretable Machine Learning in Food Analysis: Closed-Form Chemometric Calibration of Mid- and Near-Infrared Spectra Using Kolmogorov-Arnold Networks. LID - 10.3390/foods15203639 [doi] LID - 3639 AB - A Kolmogorov-Arnold network (KAN) learns a univariate function on every edge, so a trained network can be written as a closed-form equation, unlike a multilayer perceptron (MLP). A KAN was compared with five models, with selection nested within each withheld-lot or withheld-season fold, on tahini adulteration by attenuated total reflectance Fourier-transform infrared (ATR-FTIR) spectroscopy (1554 spectra, released here) and mango dry matter by near-infrared (NIR) spectroscopy (across-season test). No accuracy advantage was found: the KAN's coefficient of determination (R(2)) was comparable to a parameter-matched MLP's but never higher on average, and on withheld lots no difference between the leading models was significant. Most of the accuracy lost in converting the network to an equation was due to the conversion procedure; correcting it raised the median external R(2) of the tahini equation from 0.952 to 0.988. Repeated conversions of one network gave equations scoring -0.76 to 0.96, whereas an elementary edge basis makes the printed equation identical to the network up to rounding. With every lot serving as the blank, detection limits for samples resembling the calibration population were 8.9-21.6% adulterant, and blends at 4-12% were not quantified. The KAN provides an exact, inspectable screening calibration rather than higher accuracy. FAU - Batayhi, Abdulhamid AU - Batayhi A AUID- ORCID: 0009-0007-0374-6755 AD - Department of Mechatronics Engineering, Faculty of Mechanical Engineering, Yildiz Technical University, 34220 Istanbul, Turkiye. FAU - Ozgolet, Muhammed AU - Ozgolet M AUID- ORCID: 0000-0001-5584-097X AD - Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, 34220 Istanbul, Turkiye. FAU - Cankurt, Hasan AU - Cankurt H AD - Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, 34220 Istanbul, Turkiye. AD - Department of Nutrition and Dietetics, Faculty of Health Sciences, Biruni University, 34214 Istanbul, Turkiye. FAU - Arici, Muhammet AU - Arici M AUID- ORCID: 0000-0003-4126-200X AD - Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, 34220 Istanbul, Turkiye. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704426 OTO - NOTNLM OT - FTIR spectroscopy OT - Kolmogorov-Arnold network OT - chemometrics OT - closed-form calibration OT - food adulteration OT - interpretable machine learning OT - near-infrared spectroscopy OT - symbolic regression OT - tahini COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:36 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:36 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - foods15203639 [pii] AID - foods-15-03639 [pii] AID - 10.3390/foods15203639 [doi] PST - epublish SO - Foods. 2026 Oct 13;15(20):3639. doi: 10.3390/foods15203639. PMID- 42940268 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Biodiversity, Survival, and Influence on Quality of Yeast Biota in Virgin Olive Oil. LID - 10.3390/foods15203631 [doi] LID - 3631 AB - The microbial stability and sensory quality of extra virgin olive oil (EVOO) depend largely on its oil-borne yeast biota during storage. Virgin olive oil (VOO) harbors a low-abundance yeast biota in its aqueous microphase, whereas bacteria and molds are generally inhibited by low water activity and high phenol content. The genera Candida (6 species), Yamadazyma (5 species), Brettanomyces (2 species), Kuraishia (2 species), Nakazawaea (2 species), and Ogataea (2 species) dominate, representing 70% of reported yeast diversity. Yeast survival is strongly shaped by water content, oxygen, and phenolic concentration, with populations declining over time unless free water is available. Yeast metabolic activity can be detrimental or beneficial: lipolytic species (e.g., Candida adriatica, Yamadazyma terventina) increase free acidity and oxidative susceptibility, causing sensory defects, whereas oleuropeinolytic yeasts reduce bitterness via beta-glucosidase and esterase-mediated hydrolysis of oleuropein. Since additives and starter cultures are prohibited by EU regulations, controlling process parameters (like blending and decantation) remains the only sound strategy. As demonstrated in trials with Don Carlo and Coratina cultivars, selective management of endogenous yeasts successfully mitigates bitterness while keeping quality parameters within EVOO legal limits, thus balancing sensory profile, shelf life, and commercial value. FAU - Zullo, Biagi Angelo AU - Zullo BA AD - Department of Agricultural, Environmental and Food Sciences, University of Molise, Via De Sanctis, 86100 Campobasso, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704417 OTO - NOTNLM OT - debittering OT - extra virgin olive oil OT - lipolysis OT - oil processing OT - yeast ecology COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:35 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - foods15203631 [pii] AID - foods-15-03631 [pii] AID - 10.3390/foods15203631 [doi] PST - epublish SO - Foods. 2026 Oct 13;15(20):3631. doi: 10.3390/foods15203631. PMID- 42940267 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Development of a Chitosan/Cellulose Nanocrystal-Stabilized Pickering Emulsion Film Containing Pomelo Peel Essential Oil and Cinnamaldehyde for Strawberry Preservation. LID - 10.3390/foods15203630 [doi] LID - 3630 AB - To develop eco-friendly active packaging for food spoilage control, a Pickering emulsion (PPOEs) was prepared by co-encapsulating pomelo peel essential oil (PPO) and cinnamaldehyde (CIN) (1:1, v/v) using chitosan (CS) and carboxylated cellulose nanocrystals (C-CNCs) as composite stabilizers. Among the oil-to-water ratios tested, the emulsion prepared at 1:50 showed good storage stability and was selected for film fabrication. This combination exhibited strong antibacterial activity against four common foodborne pathogenic bacteria (E. coli, S. aureus, L. monocytogenes, and V. parahaemolyticus) and improved sensory acceptability by effectively masking the pungent odor of CIN with the fresh citrus aroma of PPO. The CS composite film containing PPOEs (CS-30%PPOEs) showed enhanced mechanical properties, UV resistance, and reduced water vapor permeability compared to the pure CS film. During strawberry preservation at 27 +/- 4 degrees C for 5 days, the film (CS-30%PPOEs) resulted in 0% decay, whereas a commercial polyvinyl chloride film (PVC; CK group) resulted in 100% decay. In addition, the composite film reduced weight loss and delayed fruit softening, thereby maintaining post-harvest quality. Soil burial tests over 21 days confirmed good biodegradability. Overall, this study provides a feasible strategy for utilizing pomelo peel waste while achieving a balance of antimicrobial efficacy, sensory quality, and environmental friendliness, offering a promising active packaging solution for controlling post-harvest spoilage of perishable fruits. FAU - Li, Juan AU - Li J AUID- ORCID: 0009-0009-1739-3756 AD - Faculty of Food and Health, Zhangzhou City Vocational College, Zhangzhou 363000, China. AD - National Engineering Research Center for Rice and Byproduct Deep Processing, School of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China. FAU - Chen, Qiao-Ling AU - Chen QL AD - School of Biological Science and Technology, Minnan Normal University, Zhangzhou 363000, China. FAU - Hu, Yong-Hua AU - Hu YH AUID- ORCID: 0000-0001-7684-5948 AD - School of Biological Science and Technology, Minnan Normal University, Zhangzhou 363000, China. FAU - Long, Zhao AU - Long Z AUID- ORCID: 0000-0001-8205-9775 AD - National Engineering Research Center for Rice and Byproduct Deep Processing, School of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China. FAU - Fu, Xiang-Jin AU - Fu XJ AD - National Engineering Research Center for Rice and Byproduct Deep Processing, School of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China. LA - eng GR - 2023J01919/Fujian Provincial Department of Science and Technology/ GR - No. JAT220701/Education Department of Fujian Province/ GR - No. ZZ2025JH04/the Natural Science Foundation of Zhangzhou/ GR - No. ZZ2025LH31/the Joint Project of the Natural Science Foundation of Zhangzhou/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13704751 OTO - NOTNLM OT - Pickering emulsion OT - active film OT - biodegradability OT - pomelo peel essential oil OT - strawberry preservation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:35 PHST- 2026/08/24 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - foods15203630 [pii] AID - foods-15-03630 [pii] AID - 10.3390/foods15203630 [doi] PST - epublish SO - Foods. 2026 Oct 13;15(20):3630. doi: 10.3390/foods15203630. PMID- 42940266 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - Automated and Highly Efficient Detection of Lignans in Plant-Derived Foods Based on Magnetic Solid-Phase Extraction Combined with UPLC. LID - 10.3390/foods15203629 [doi] LID - 3629 AB - Lignans, a class of plant-derived polyphenolic compounds, exhibit significant bioactivities and serve as valuable functional food ingredients. However, conventional enrichment methods often suffer from inadequate selectivity and environmental incompatibility, limiting their practical application. In this study, the magnetic metal-organic framework Fe(3)O(4)@ZIF-67 was employed as an adsorbent for magnetic solid-phase extraction (MSPE). Coupled with ultra-performance liquid chromatography (UPLC), this method achieved rapid, selective, and high-efficiency enrichment of lignans-sesaminol, sesamin, and sesamolin. Method validation demonstrated excellent linearity (R(2) >/= 0.9999) over the concentration range of 0.5-100 mug/mL, acceptable recoveries (75.7-107.8%) with relative standard deviations (RSDs) /=6% hydrocolloid, particularly XG:KG ratios between 1:2 to 3:1 demonstrated superior print accuracy (>94%) and met all three dysphagia classifications. Numerical optimization predicted an optimum formulation of 5.263% XG and 3.188% KG with an overall desirability of 0.91. These findings provide a formulation strategy for developing sustainable, dysphagia-friendly 3D-printed foods from C. glomerata. FAU - Phyu, Yonn Pwint AU - Phyu YP AD - Food Science and Technology Program, School of Agro-Industry, Mae Fah Luang University, Chiang Rai 57100, Thailand. FAU - Rawdkuen, Saroat AU - Rawdkuen S AUID- ORCID: 0000-0001-6664-3031 AD - Food Science and Technology Program, School of Agro-Industry, Mae Fah Luang University, Chiang Rai 57100, Thailand. AD - Research Centre of Innovative Food Packing and Biomaterials, Mae Fah Luang University, Chiang Rai 57100, Thailand. FAU - Konsue, Nattaya AU - Konsue N AUID- ORCID: 0000-0001-8367-370X AD - Food Science and Technology Program, School of Agro-Industry, Mae Fah Luang University, Chiang Rai 57100, Thailand. AD - Research Centre of Innovative Food Packing and Biomaterials, Mae Fah Luang University, Chiang Rai 57100, Thailand. LA - eng GR - 692A04028/National Science, Research and Innovation Fund (NSRF)/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13703241 OTO - NOTNLM OT - 3D food printing OT - Cladophora glomerata OT - dysphagia OT - konjac gum OT - response surface methodology OT - texture-modified foods OT - xanthan gum COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:35 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - foods15203595 [pii] AID - foods-15-03595 [pii] AID - 10.3390/foods15203595 [doi] PST - epublish SO - Foods. 2026 Oct 10;15(20):3595. doi: 10.3390/foods15203595. PMID- 42940224 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 9 TI - Stir-Frying Reshapes the Chemical Profile and Metabolic Effects of Raphani Semen in Diabetic Mice: Integrated Analysis of Hepatic Metabolism and Gut Microbiota. LID - 10.3390/foods15203587 [doi] LID - 3587 AB - Stir-frying can alter the chemical composition and biological functionality of Raphani Semen (RS), but whether this processing improves its glucose-regulating properties remains unclear. In this study, a representative laboratory-scale stir-frying condition (160 degrees C for 3 min) was selected through exploratory chromatographic, macroscopic, and organoleptic assessment. Raw RS and stir-fried Raphani Semen (SF-RS) were subsequently compared using chemical and serum-exposure profiling, metabolic phenotyping, liver histopathology, hepatic proteomics and metabolomics, gut microbiota analysis, and targeted molecular validation in a diet/streptozotocin-induced mouse model of type 2 diabetes. Stir-frying altered relative LC-MS chemical response patterns and serum-detectable feature profiles, with selected glucosinolate-related signals showing the most consistent processing-associated differences. Functionally, SF-RS produced significantly greater reductions in terminal fasting glucose, insulin, and HOMA-IR than raw RS at all three matched doses (p < 0.05), while its advantage in OGTT AUC was particularly evident at the low dose. SF-RS also enhanced hepatic PAS-positive glycogen restoration across the tested dose range and improved lipid accumulation and collagen deposition at selected doses. Multi-omics analyses indicated processing-dependent regulation of PI3K-Akt/FoxO, PPAR, lipid, and cofactor metabolic pathways, together with distinct remodeling of the gut microbial community. Targeted validation further showed stronger regulation of PI3K-Akt signaling, Forkhead Box O1 (FoxO1) nuclear accumulation, and PPAR-alpha/CPT1A/ACOX1-associated fatty-acid metabolism after stir-frying. These findings indicate that thermal processing strengthens the glucose- and insulin-related functionality of Raphani Semen and enhances its potential value as a functional food ingredient for metabolic health. FAU - Bai, Chenxi AU - Bai C AD - Key Laboratory of Basis and Application Research of Beiyao, Ministry of Education, Heilongjiang University of Chinese Medicine, Harbin 150040, China. FAU - Su, Fazhi AU - Su F AD - Key Laboratory of Basis and Application Research of Beiyao, Ministry of Education, Heilongjiang University of Chinese Medicine, Harbin 150040, China. FAU - Sun, Minghao AU - Sun M AD - Key Laboratory of Basis and Application Research of Beiyao, Ministry of Education, Heilongjiang University of Chinese Medicine, Harbin 150040, China. FAU - Liu, Meng AU - Liu M AD - Key Laboratory of Basis and Application Research of Beiyao, Ministry of Education, Heilongjiang University of Chinese Medicine, Harbin 150040, China. AD - School of Chinese Materia Medica and Health, Bozhou University, Bozhou 236800, China. FAU - Sun, Yanping AU - Sun Y AD - Key Laboratory of Basis and Application Research of Beiyao, Ministry of Education, Heilongjiang University of Chinese Medicine, Harbin 150040, China. FAU - Wang, Qiuhong AU - Wang Q AD - Key Laboratory of Basis and Application Research of Beiyao, Ministry of Education, Heilongjiang University of Chinese Medicine, Harbin 150040, China. AD - School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China. FAU - Kuang, Haixue AU - Kuang H AUID- ORCID: 0000-0001-9136-8414 AD - Key Laboratory of Basis and Application Research of Beiyao, Ministry of Education, Heilongjiang University of Chinese Medicine, Harbin 150040, China. LA - eng GR - 2018YFC1707100/China National Center for Biotechnology Development/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13703343 OTO - NOTNLM OT - Raphani Semen OT - functional food OT - glucose homeostasis OT - insulin resistance OT - multi-omics OT - stir-frying OT - thermal processing COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:35 PHST- 2026/08/28 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - foods15203587 [pii] AID - foods-15-03587 [pii] AID - 10.3390/foods15203587 [doi] PST - epublish SO - Foods. 2026 Oct 9;15(20):3587. doi: 10.3390/foods15203587. PMID- 42940220 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 9 TI - Coffeehouses as Symbolic Spaces: Materialism and Experiential Motivations in Coffeehouse Selection. LID - 10.3390/foods15203585 [doi] LID - 3585 AB - The demand for coffeehouses has been growing globally; however, little is known about the psychological factors associated with individuals' coffeehouse selection. Drawing on a value-motivation-intention framework, this study examines how materialism-coffeehouse as defining success (M-CDS), as an underlying value orientation, relates to snob, bandwagon, hedonic consumption tendency (HCT), and eudaimonic orientations, and how these orientations are associated with customers' willingness to select coffeehouses. Survey data collected from Turkiye (n = 286) and Italy (n = 315) were analyzed using PLS-SEM. The findings showed that M-CDS was positively associated with all four psychological orientations, each of which was positively associated with willingness to select coffeehouses. Significant indirect effects were also observed through all four orientations, with the eudaimonic pathway showing the largest specific indirect effect. Multi-group analysis identified several between-country differences: the relationship between eudaimonic motivation and willingness was stronger among Italian participants, whereas the relationship between HCT and willingness was stronger among Turkish participants. These findings indicate that coffeehouse selection is associated not only with functional considerations but also with consumers' value orientations and social and experiential motivations. The study extends previous research by incorporating the eudaimonic pathway and examining these relationships across Turkish and Italian samples, while offering practical insights for coffeehouse managers operating across different markets. FAU - Ucuk, Ceyhun AU - Ucuk C AUID- ORCID: 0000-0003-2809-6430 AD - Department of Experimental Psychology, University of Oxford, Life and Mind Building, South Parks Road, Oxford OX1 3EL, UK. AD - Department of Gastronomy and Culinary Arts, Faculty of Tourism, Gaziantep University, 27100 Gaziantep, Turkiye. FAU - Kement, Uzeyir AU - Kement U AD - Department of Gastronomy and Culinary Arts, Faculty of Tourism, Ordu University, 52200 Ordu, Turkiye. FAU - Migliavada, Riccardo AU - Migliavada R AUID- ORCID: 0000-0002-4262-8360 AD - University of Gastronomic Sciences, Piazza Vittorio Emanuele II 9, 12042 Pollenzo, Italy. FAU - Sahin, Nilufer AU - Sahin N AD - Department of Gastronomy and Culinary Arts, Faculty of Tourism, Nevsehir Haci Bektas Veli University, 50100 Nevsehir, Turkiye. FAU - Arman, Onurcan AU - Arman O AUID- ORCID: 0009-0000-4675-5466 AD - School of Exercise and Nutrition Sciences, Faculty of Health, Deakin University, Melbourne 3125, Australia. FAU - Torri, Luisa AU - Torri L AUID- ORCID: 0000-0003-4522-6452 AD - University of Gastronomic Sciences, Piazza Vittorio Emanuele II 9, 12042 Pollenzo, Italy. FAU - Spence, Charles AU - Spence C AUID- ORCID: 0000-0003-2111-072X AD - Department of Experimental Psychology, University of Oxford, Life and Mind Building, South Parks Road, Oxford OX1 3EL, UK. LA - eng GR - AHRC grant 'Rethinking the senses' (Grant Number: AH/L007053/1)./UK Research and Innovation/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13703210 OTO - NOTNLM OT - PLS-SEM OT - coffee OT - coffeehouse OT - consumer behavior OT - cross-cultural analysis OT - materialism COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:35 PHST- 2026/08/23 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - foods15203585 [pii] AID - foods-15-03585 [pii] AID - 10.3390/foods15203585 [doi] PST - epublish SO - Foods. 2026 Oct 9;15(20):3585. doi: 10.3390/foods15203585. PMID- 42940219 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2304-8158 (Print) IS - 2304-8158 (Electronic) IS - 2304-8158 (Linking) VI - 15 IP - 20 DP - 2026 Oct 9 TI - Baobab (Adansonia digitata L.) Fruit Pulp in Nutritional Therapeutics: Mechanistic Insights, Clinical Evidence, and Future Research Priorities. LID - 10.3390/foods15203579 [doi] LID - 3579 AB - The renewed interest in indigenous African foods reflects a shift towards traditional, nutrient-dense alternatives to address the burden of non-communicable diseases and micronutrient deficiencies. This review evaluates the evidence for proposed health benefits of baobab (Adansonia digitata L.) fruit pulp (BFP) across four domains: gut homeostasis, immune modulation, glycemic control, and iron bioavailability. The evidence base is predominantly preclinical. Tier 3 (in vitro) and Tier 2 (animal) studies suggest that BFP fibers (low-methoxylated homogalacturonan, xylogalacturonan, and possibly inulin and fructooligosaccharides) may exert prebiotic-like effects by selectively stimulating Bifidobacteriaceae and Lactobacilli, while BFP polyphenols and organic acids have been associated with enzyme inhibition and enhanced iron bioaccessibility, respectively. The methodological quality of these studies was not assessed. However, human evidence is scarce. Only six human intervention studies were identified, limited to glycemic response in healthy normoglycemic adults and one iron status trial in schoolchildren, which showed increased hemoglobin but no significant improvement in ferritin or soluble transferrin receptor. No human studies have examined gut microbiota modulation, immune outcomes, or effects in diabetic or pre-diabetic populations. Consequently, clinical efficacy has not been established, and BFP cannot currently be characterized as having confirmed prebiotic or therapeutic properties. The current evidence supports potential prebiotic and enzyme-inhibitory activity in preclinical models only. Evidence-based dietary recommendations require high-quality human intervention trials with clinically meaningful endpoints, standardization of BFP preparations, and systematic investigation of real-world dietary contexts. Future research should address these gaps to advance the evidence base for BFP. Practical barriers to translation, including sensory acceptance, product adulteration, and sustainable sourcing, are briefly considered. FAU - Pfukwa, Trust Mukudzei AU - Pfukwa TM AUID- ORCID: 0000-0001-9362-2615 AD - Postharvest and Agroprocessing Research Centre, Department of Botany and Plant Biotechnology, University of Johannesburg, Auckland Park, Johannesburg 2006, South Africa. AD - South African Research Chairs Initiative in Sustainable Preservation and Agroprocessing Research, Faculty of Science, University of Johannesburg, P.O. Box 524, Auckland Park, Johannesburg 2006, South Africa. FAU - Abdulraheem, Mukhtar Iderawumi AU - Abdulraheem MI AUID- ORCID: 0000-0002-6745-378X AD - Postharvest and Agroprocessing Research Centre, Department of Botany and Plant Biotechnology, University of Johannesburg, Auckland Park, Johannesburg 2006, South Africa. AD - South African Research Chairs Initiative in Sustainable Preservation and Agroprocessing Research, Faculty of Science, University of Johannesburg, P.O. Box 524, Auckland Park, Johannesburg 2006, South Africa. FAU - Aremu, Adeyemi O AU - Aremu AO AUID- ORCID: 0000-0002-6602-246X AD - Centre of Excellence in Indigenous Knowledge Systems, Faculty of Natural and Agricultural Sciences, North-West University, Private Bag X2046, Mmabatho 2745, South Africa. AD - South African Research Chairs Initiative in Indigenous Knowledge-Driven Medicinal Plants Utilisation and Conservation Strategies for Human, Animal, and Crop Health (IK-Medplants4HAC), Faculty of Natural and Agricultural Sciences, North-West University, Private Bag X2046, Mmabatho 2745, South Africa. FAU - Fawole, Olaniyi A AU - Fawole OA AUID- ORCID: 0000-0001-5591-4633 AD - Postharvest and Agroprocessing Research Centre, Department of Botany and Plant Biotechnology, University of Johannesburg, Auckland Park, Johannesburg 2006, South Africa. AD - South African Research Chairs Initiative in Sustainable Preservation and Agroprocessing Research, Faculty of Science, University of Johannesburg, P.O. Box 524, Auckland Park, Johannesburg 2006, South Africa. LA - eng GR - RCHDI2411105279212;SPAR231013155231/National Research Foundation/ PT - Journal Article PT - Review DEP - 20261009 PL - Switzerland TA - Foods JT - Foods (Basel, Switzerland) JID - 101670569 PMC - PMC13702918 OTO - NOTNLM OT - baobab fruit pulp OT - gut homeostasis OT - immune system support OT - indigenous fruit OT - nutritional transition OT - symbiotic COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:35 PHST- 2026/09/20 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - foods15203579 [pii] AID - foods-15-03579 [pii] AID - 10.3390/foods15203579 [doi] PST - epublish SO - Foods. 2026 Oct 9;15(20):3579. doi: 10.3390/foods15203579. PMID- 42940214 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 21 TI - Axillary Dacron Graft Versus Direct Femoral Arterial Cannulation for Peripheral VA-ECMO After Heart Transplantation: A Single-Center Cohort Study. LID - 10.3390/biomedicines14102408 [doi] LID - 2408 AB - Background/Objectives: Primary graft dysfunction (PGD) remains a major cause of early morbidity and mortality after heart transplantation, and peripheral veno-arterial extracorporeal membrane oxygenation (VA-ECMO) represents an established rescue therapy for severe cases. Although direct femoral arterial cannulation is commonly used because of its rapid deployment, axillary artery cannulation through a Dacron graft provides antegrade arterial perfusion and may represent an alternative approach. This study compared early clinical outcomes and cannulation-related complications between these two arterial cannulation strategies. Methods: In this retrospective single-center observational study, 42 consecutive adult heart transplant recipients requiring peripheral VA-ECMO for PGD were included. Patients were divided according to arterial cannulation strategy into direct femoral cannulation (DFC, n = 25) and axillary Dacron graft cannulation (ADG, n = 17). Baseline recipient and donor characteristics, perioperative variables, postoperative outcomes, vascular complications, successful VA-ECMO weaning, and survival to hospital discharge were compared. Odds ratios (ORs) with 95% confidence intervals (CIs) were calculated for major clinical outcomes and cannulation-related complications. Results: The primary composite endpoint of cannulation-related complications occurred in 0/17 patients (0%) in the ADG group and 3/25 patients (12.0%) in the DFC group (OR 0.18, 95% CI 0.01-3.80; p = 0.260). Successful VA-ECMO weaning was achieved in 94.1% versus 92.0% (p = 1.000), and survival to hospital discharge was 76.5% versus 64.0% (OR 1.83, 95% CI 0.46-7.32; p = 0.505). Stroke occurred in 5.9% versus 16.0% (OR 0.33, 95% CI 0.03-3.23; p = 0.632), while renal replacement therapy was required in 35.3% versus 52.0% (OR 0.50, 95% CI 0.14-1.79; p = 0.353). No statistically significant differences were detected between groups. Conclusions: No statistically significant differences in successful VA-ECMO weaning or survival to hospital discharge were observed between axillary Dacron graft and direct femoral arterial cannulation. No statistically significant differences in neurological, vascular, or other major postoperative outcomes were detected. Although several complications occurred numerically less frequently with axillary cannulation, the limited sample size and wide confidence intervals preclude conclusions regarding comparative superiority. Axillary Dacron graft cannulation may therefore represent a feasible alternative in selected heart transplant recipients requiring peripheral VA-ECMO for PGD. Larger prospective multicenter studies are warranted. FAU - Giovannico, Lorenzo AU - Giovannico L AD - Cardiac Surgery Unit, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari Aldo Moro, Piazza Giulio Cesare 11, 70124 Bari, Italy. FAU - Fischetti, Giuseppe AU - Fischetti G AD - Cardiac Surgery Unit, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari Aldo Moro, Piazza Giulio Cesare 11, 70124 Bari, Italy. FAU - Parigino, Domenico AU - Parigino D AD - Cardiac Surgery Unit, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari Aldo Moro, Piazza Giulio Cesare 11, 70124 Bari, Italy. FAU - Savino, Luca AU - Savino L AD - Cardiac Surgery Unit, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari Aldo Moro, Piazza Giulio Cesare 11, 70124 Bari, Italy. FAU - Leo, Claudia AU - Leo C AD - Cardiac Surgery Unit, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari Aldo Moro, Piazza Giulio Cesare 11, 70124 Bari, Italy. FAU - Cristiano, Giuseppe AU - Cristiano G AD - Cardiac Surgery Unit, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari Aldo Moro, Piazza Giulio Cesare 11, 70124 Bari, Italy. FAU - Paradiso, Ilaria AU - Paradiso I AD - Cardiac Surgery Unit, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari Aldo Moro, Piazza Giulio Cesare 11, 70124 Bari, Italy. FAU - Ferrara, Federica AU - Ferrara F AD - Cardiac Surgery Unit, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari Aldo Moro, Piazza Giulio Cesare 11, 70124 Bari, Italy. FAU - Broggini, Francesca AU - Broggini F AD - Cardiac Surgery Unit, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari Aldo Moro, Piazza Giulio Cesare 11, 70124 Bari, Italy. FAU - Mancini, Carlo AU - Mancini C AD - Cardiac Surgery Unit, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari Aldo Moro, Piazza Giulio Cesare 11, 70124 Bari, Italy. FAU - Carrozzini, Massimiliano AU - Carrozzini M AD - Cardiac Surgery Unit, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari Aldo Moro, Piazza Giulio Cesare 11, 70124 Bari, Italy. FAU - Padalino, Massimo AU - Padalino M AUID- ORCID: 0000-0002-7535-9670 AD - Cardiac Surgery Unit, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari Aldo Moro, Piazza Giulio Cesare 11, 70124 Bari, Italy. FAU - Bottio, Tomaso AU - Bottio T AD - Cardiac Surgery Unit, Department of Precision and Regenerative Medicine and Ionian Area (DiMePRe-J), University of Bari Aldo Moro, Piazza Giulio Cesare 11, 70124 Bari, Italy. LA - eng GR - PNRR-MCNT2-2023-12378350/Ministry of Health/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13702244 OTO - NOTNLM OT - axillary artery cannulation OT - femoral artery cannulation OT - heart transplantation OT - primary graft dysfunction OT - venoarterial extracorporeal membrane oxygenation (VA-ECMO) COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:35 PHST- 2026/08/06 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - biomedicines14102408 [pii] AID - biomedicines-14-02408 [pii] AID - 10.3390/biomedicines14102408 [doi] PST - epublish SO - Biomedicines. 2026 Oct 21;14(10):2408. doi: 10.3390/biomedicines14102408. PMID- 42940212 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 21 TI - Genetically Proxied SGLT2 Inhibition and Risk of Epilepsy: A Mediation Mendelian Randomization Study of Circulating Metabolites. LID - 10.3390/biomedicines14102406 [doi] LID - 2406 AB - Background: Epilepsy is a chronic neurological disorder that can significantly impair quality of life. Previous studies have demonstrated the positive effects of sodium-glucose co-transporter 2 (SGLT2) inhibitors on various systems, including the nervous system. SGLT2 inhibitors have been shown to reduce seizure activity in pentylenetetrazol-induced epilepsy models in rats. Recent study results showed that glucose-lowering drugs including SGLT2 inhibitors reduced risk of seizures and epilepsy. Methods: Genetic variants of SGLT2 inhibition were selected as exposure instrumental variables (IVs) and used type 2 diabetes (T2DM) as validation. Two-sample Mendelian Randomization (MR) analysis was conducted to explore the causal relationship between SGLT2 inhibition and epilepsy. In addition, we performed a two-step MR to evaluate whether possible circulating metabolites mediate the effects of SGLT2 inhibition on epilepsy. Results: The two-sample MR analysis showed that genetically proxied SGLT2 inhibition was associated with lower odds of epilepsy under MR assumptions [IVW: OR = 0.30 (95% CI: 0.13, 0.71), p = 0.0064]. Exploratory two-step analyses identified 3-methylglutaconate and N-acetylleucine as candidate metabolic signals; however, neither signal remained statistically significant after Benjamini-Hochberg correction. Conclusion: Our study suggests that SGLT2 is associated with a reduced risk of epilepsy, and the underlying mechanism may be the potential antioxidant and anti-inflammatory effects of SGLT2. In addition, circulating 3-methylglutaconate and N-acetylleucine should therefore be regarded as candidate metabolic signals rather than established mediators. FAU - Chang, Mei AU - Chang M AD - Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China. FAU - Li, Jiaxuan AU - Li J AD - Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China. FAU - Gu, Feng AU - Gu F AD - Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China. FAU - Wu, Xin AU - Wu X AD - Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China. FAU - Song, Bingyi AU - Song B AD - Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China. FAU - Chen, Zhouqing AU - Chen Z AUID- ORCID: 0000-0001-7517-7036 AD - Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China. FAU - Wang, Zhong AU - Wang Z AUID- ORCID: 0000-0002-7342-0845 AD - Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, Suzhou 215006, China. LA - eng GR - 82201445/National Natural Science Foundation of China/ GR - 82171309/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13702378 OTO - NOTNLM OT - Mendelian randomization analysis OT - SGLT2 inhibition OT - diabetes OT - epilepsy OT - pharmacological targets COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:35 PHST- 2026/09/29 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - biomedicines14102406 [pii] AID - biomedicines-14-02406 [pii] AID - 10.3390/biomedicines14102406 [doi] PST - epublish SO - Biomedicines. 2026 Oct 21;14(10):2406. doi: 10.3390/biomedicines14102406. PMID- 42940211 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 21 TI - The Translational Value of Preclinical Research: Lessons from More Than Forty Years of Retinal Research. LID - 10.3390/biomedicines14102405 [doi] LID - 2405 AB - The present paper is based on a long personal experience of research on the retina. Over almost forty years, questions that were originally pursued as curiosity-driven physiology progressively acquired unexpected translational implications. The retina provides a particularly instructive setting in which to examine translational potential because its accessible and highly organized neural and vascular architecture has allowed mechanisms identified in experimental models to be tested as potential therapeutic targets. Three research trajectories from our laboratory are discussed as examples of different translational outcomes. Studies of the somatostatinergic system illustrate how a strong mechanistic rationale and convincing preclinical efficacy may not necessarily translate into comparable clinical benefit. Investigations of the uPA/uPAR system and its pharmacological inhibition show how a candidate that does not reach clinical application may nevertheless provide durable insight into disease mechanisms and identify biologically relevant pathways. Conversely, studies of beta-adrenoceptor signaling and propranolol in retinopathy of prematurity illustrate how drug repurposing can shorten the distance between experimental evidence and clinical investigation, while still leaving important questions about dose, safety, and delivery. These experiences also highlight model selection, disease stage, pharmacokinetics, tissue exposure, reproducibility and patient heterogeneity as critical determinants of translational success. Taken together, my experience of research on the retina suggests that translation should be viewed not as a one-way progression from bench to bedside, but as an iterative process in which experimental findings, clinical outcomes and even unsuccessful therapeutic attempts continuously reshape biological questions. FAU - Bagnoli, Paola AU - Bagnoli P AUID- ORCID: 0000-0001-6659-9077 AD - Department of Biology, University of Pisa, 56127 Pisa, Italy. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701875 OTO - NOTNLM OT - drug delivery OT - drug repurposing OT - preclinical models OT - retinal research OT - somatostatin OT - translational medicine OT - uPA/uPAR OT - beta-adrenoceptors COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:35 PHST- 2026/09/24 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - biomedicines14102405 [pii] AID - biomedicines-14-02405 [pii] AID - 10.3390/biomedicines14102405 [doi] PST - epublish SO - Biomedicines. 2026 Oct 21;14(10):2405. doi: 10.3390/biomedicines14102405. PMID- 42940208 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 21 TI - Therapeutic Interventions in Niemann-Pick Disease Type C: A Systematic Review of Clinical Outcomes and Safety. LID - 10.3390/biomedicines14102402 [doi] LID - 2402 AB - Background: Niemann-Pick disease type C (NPC) is a rare, progressive lysosomal storage disorder characterized by impaired intracellular lipid trafficking, leading to systemic, neurological, and psychiatric manifestations. Although supportive care was historically the main treatment approach, several disease-modifying and symptomatic therapies have recently emerged. This systematic review aimed to evaluate the efficacy and safety of current therapeutic strategies for NPC. Methods: A systematic literature search was conducted in PubMed, Scopus, and ClinicalTrials.gov from inception to 1 August 2026. Eligible studies included randomized controlled trials and interventional clinical studies involving patients with NPC of any age or sex. Interventions included miglustat, arimoclomol, N-acetyl-L-leucine (NALL), hydroxypropyl-beta-cyclodextrin (HPbetaCD), vorinostat, N-acetylcysteine (NAC), efavirenz, nizubaglustat and other therapeutic strategies. Outcomes included neurological progression, clinical severity scores, ataxia measures, quality of life, biomarkers, cognition, and adverse events. Due to heterogeneity in study designs and outcome measures, findings were synthesized narratively. Results: Twenty-four studies representing approximately 475 participants, after removal of documented cohort overlap, were included. Miglustat showed evidence of neurological stabilization, particularly when initiated early, with mainly gastrointestinal adverse effects. Arimoclomol demonstrated evidence of slowing neurological progression and was generally well tolerated, although longer-term safety and effectiveness data remain necessary. NALL improved ataxia-related symptoms and was generally well tolerated. HPbetaCD demonstrated biological target engagement, including changes in cholesterol-related biomarkers, but clinical findings were mixed: uncontrolled intrathecal and intravenous studies suggested stabilization or improvement, whereas controlled evidence was less supportive. Hearing impairment remained an important safety concern. Evidence for vorinostat, NAC, efavirenz, and nizubaglustat was limited by small samples and early-phase or uncontrolled designs. Conclusions: The available evidence most consistently supports miglustat, arimoclomol, and NALL for stabilizing neurological progression or improving functional outcomes in NPC. However, differences in study design, outcome measures, and follow-up preclude direct comparison between therapies. HPbetaCD remains investigational because its biological activity has not translated into consistent controlled clinical benefit and requires careful safety monitoring. Larger controlled studies using standardized clinical outcomes and reliable biomarkers are needed to define long-term effectiveness and optimize treatment selection. FAU - Nafees, Zaina AU - Nafees Z AD - RAK College of Medical Sciences, RAK Medical and Health Sciences University, Ras Al Khaimah 11172, United Arab Emirates. FAU - Mohammed, Sara S N AU - Mohammed SSN AD - RAK College of Medical Sciences, RAK Medical and Health Sciences University, Ras Al Khaimah 11172, United Arab Emirates. FAU - Bernhardt, Grisilda Vidya AU - Bernhardt GV AD - Department of Biochemistry, RAK College of Medical Sciences, RAK Medical and Health Sciences University, Ras Al Khaimah 11172, United Arab Emirates. FAU - Kumar, Suresh AU - Kumar S AD - Department of Pharmacology, RAK College of Medical Sciences, RAK Medical and Health Sciences University, Ras Al Khaimah 11172, United Arab Emirates. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13702622 OTO - NOTNLM OT - N-acetyl-L-leucine OT - NPC OT - Niemann-Pick disease type C OT - arimoclomol OT - efavirenz OT - hydroxypropyl-beta-cyclodextrin OT - lysosomal storage disorder OT - miglustat OT - nizubaglustat OT - systematic review COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:35 PHST- 2026/06/12 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - biomedicines14102402 [pii] AID - biomedicines-14-02402 [pii] AID - 10.3390/biomedicines14102402 [doi] PST - epublish SO - Biomedicines. 2026 Oct 21;14(10):2402. doi: 10.3390/biomedicines14102402. PMID- 42940206 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 21 TI - Phenotypic Landscape of Programmed Death and TIGIT/CD155/DNAM-1 Pathways in NK Cells in Ovarian Cancer. LID - 10.3390/biomedicines14102400 [doi] LID - 2400 AB - Objectives: Ovarian cancer (OC) is the gynecological malignancy with the highest mortality rate. Preclinical studies have shown that dual blockade of immune checkpoint proteins (ICPs), such as PD-1 and TIGIT, may be beneficial for OC patients. Thus, the aim of this study was to evaluate the expression of PD-1, TIGIT, and DNAM-1 in NK cells in three OC environments-i.e., peripheral blood (PB), peritoneal fluid (PF), and tumor tissue (TT)-and their soluble forms in plasma and PF to explore their clinical significance. Methods: The expression of ICPs on NK cells was analyzed via flow cytometry. The concentrations of soluble forms of these ICPs were measured in plasma and PF via ELISA. The obtained results were correlated with the clinicopathological features of OC patients. Results: We observed an increased percentage of PD-1(+) NK cells in tumor tissue (p < 0.05), as well as TIGIT(+) (p < 0.001) and DNAM-1(+) NK cells in PF of patients with OC (p < 0.05). Soluble PD-1 (sPD-1) concentration was higher in PF than in plasma (p < 0.0001) and higher in plasma of OC patients than in controls (p < 0.001), while soluble TIGIT (sTIGIT) levels were elevated in plasma of OC patients compared with controls (p < 0.05). NK cell phenotypic alterations were also associated with selected clinicopathological features of OC (i.e., FIGO disease advancement, tumor type according to Kurman and Shih, and grade). The percentage of NK cells in PF was positively associated with age (p < 0.01) and negatively associated with BMI (p < 0.05). Conclusions: OC is characterized by distinct compartment-specific patterns of PD-1, TIGIT, and DNAM-1 expression on NK cells, accompanied by altered soluble ICP profiles. These phenotypic patterns may help define the immune landscape of the ovarian cancer microenvironment. FAU - Pawlowska-Lachut, Anna AU - Pawlowska-Lachut A AUID- ORCID: 0000-0003-1532-2662 AD - Independent Laboratory of Cancer Diagnostics and Immunology, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland. FAU - Skiba, Wiktoria AU - Skiba W AUID- ORCID: 0000-0002-9599-6773 AD - Independent Laboratory of Cancer Diagnostics and Immunology, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland. FAU - Pieniadz-Feculak, Paulina AU - Pieniadz-Feculak P AD - Independent Laboratory of Cancer Diagnostics and Immunology, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland. AD - Department of Virology and Immunology, Institute of Biological Sciences, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland. FAU - Wlodarczyk-Ciekanska, Karolina AU - Wlodarczyk-Ciekanska K AUID- ORCID: 0000-0001-6478-6370 AD - Independent Laboratory of Cancer Diagnostics and Immunology, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland. FAU - Suszczyk, Dorota AU - Suszczyk D AUID- ORCID: 0000-0003-1022-3323 AD - Independent Laboratory of Cancer Diagnostics and Immunology, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland. FAU - Lyczba, Jakub AU - Lyczba J AUID- ORCID: 0000-0002-3420-4832 AD - Second Department of Gynecological Oncology, St. John's Center of Oncology of the Lublin Region, 20-090 Lublin, Poland. FAU - Bilska, Monika AU - Bilska M AD - Second Department of Gynecological Oncology, St. John's Center of Oncology of the Lublin Region, 20-090 Lublin, Poland. FAU - Kazimierczuk, Michalina AU - Kazimierczuk M AD - Second Department of Gynecological Oncology, St. John's Center of Oncology of the Lublin Region, 20-090 Lublin, Poland. FAU - Kotarski, Jozef AU - Kotarski J AD - Second Department of Gynecological Oncology, St. John's Center of Oncology of the Lublin Region, 20-090 Lublin, Poland. FAU - Boniewska-Bernacka, Ewa AU - Boniewska-Bernacka E AD - Department of Biology and Genetics, Institute of Medical Sciences, University of Opole, Oleska 48, 45-052 Opole, Poland. FAU - Tomaszewski, Jacek AU - Tomaszewski J AD - Second Department of Gynecological Oncology, St. John's Center of Oncology of the Lublin Region, 20-090 Lublin, Poland. FAU - Wertel, Iwona AU - Wertel I AUID- ORCID: 0000-0003-3396-1679 AD - Independent Laboratory of Cancer Diagnostics and Immunology, Medical University of Lublin, Chodzki 1, 20-093 Lublin, Poland. LA - eng GR - 2021/41/N/NZ6/01727/National Science Centre/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13702272 OTO - NOTNLM OT - DNAM-1 OT - PD-1 OT - TIGIT OT - dual blockade OT - ovarian cancer OT - tumor microenvironment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:35 PHST- 2026/08/17 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - biomedicines14102400 [pii] AID - biomedicines-14-02400 [pii] AID - 10.3390/biomedicines14102400 [doi] PST - epublish SO - Biomedicines. 2026 Oct 21;14(10):2400. doi: 10.3390/biomedicines14102400. PMID- 42940198 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 19 TI - Decision Limits for Fetal Speckle-Tracking Outputs: Minimal Detectable Change and Threshold Instability in FetalHQ. LID - 10.3390/biomedicines14102392 [doi] LID - 2392 AB - Background: Fetal Heart Quantification (FetalHQ) enables detailed assessment of fetal cardiac morphology and function using two-dimensional speckle-tracking echocardiography. However, previous studies have focused primarily on conventional reproducibility metrics such as the intraclass correlation coefficient (ICC). From a clinical perspective, it is more relevant to determine what magnitude of change exceeds measurement noise and whether a single result may lead to unstable classification relative to normative thresholds. The aim of this study was to evaluate the decision limits of selected FetalHQ parameters, including the minimal detectable change (MDC95), threshold instability, and the theoretical effect of averaging two independent analyses. Materials and Methods: This methodological study included 93 paired analyses (_1 and _2) obtained from the same recorded four-chamber cine loop under the assumption of zero biological variability. The intra-operator subset (n = 34) and inter-operator subset (n = 59) were analyzed separately. For key parameters, we determined the proportion of usable pairs after quality filtering, bias, Bland-Altman 95% limits of agreement, MDC95, classification flip rates for normalized outputs, and the width of the uncertainty zone (grey zone) around diagnostic thresholds. In addition, we estimated the effect of a mean-of-two workflow on measurement error reduction. Results: The best analytical performance was observed for left ventricular volumetric parameters, particularly LV EDV and LV ESV, which showed the highest proportion of usable pairs (88.2-94.9%) and relatively low MDC95 values. Functional and deformation metrics performed substantially worse, including LV EF, LV FRAC, LV GLS, and RV TAPSE, all of which were characterized by lower usability and wider limits of agreement, especially in the inter-operator analysis. Classification instability was greatest for normalized parameters. In the inter-operator analysis, classification flips occurred in 33.3% of cases for LV EF centile <5, 31.4% for RV TAPSE lateral centile <5, 35.7% for LV GLS centile >95, and 39.2% for LV GLS Z-score >2. Under the theoretical assumption of independent random measurement error, the estimated MDC95 for the mean of two analyses was approximately 29% lower than that for a single analysis; this reduction was mathematically derived rather than prospectively observed. Conclusions: FetalHQ showed the highest analytical stability for simpler LV volumetric parameters, whereas functional and deformation outputs remained substantially more susceptible to measurement noise and classification instability, particularly between operators. In practice, this means that a single measurement obtained near normative thresholds may be insufficient for reliable interpretation. A theoretical mean-of-two approach may reduce random measurement uncertainty, but this strategy was not prospectively validated and does not overcome the limitations of the least analytically stable parameters. FAU - Mlodawski, Jakub AU - Mlodawski J AUID- ORCID: 0000-0002-5740-9822 AD - Institute of Medical Sciences, Collegium Medicum, Jan Kochanowski University in Kielce, 25-516 Kielce, Poland. AD - Clinic of Obstetrics and Gynecology, Provincial Combined Hospital in Kielce, 25-736 Kielce, Poland. FAU - Zmelonek-Znamirowska, Anna AU - Zmelonek-Znamirowska A AUID- ORCID: 0000-0002-6610-7965 AD - Institute of Medical Sciences, Collegium Medicum, Jan Kochanowski University in Kielce, 25-516 Kielce, Poland. AD - Clinic of Obstetrics and Gynecology, Provincial Combined Hospital in Kielce, 25-736 Kielce, Poland. FAU - Pawlik, Lukasz AU - Pawlik L AUID- ORCID: 0000-0003-0289-2527 AD - Department of Information Systems, Kielce University of Technology, 25-314 Kielce, Poland. FAU - Mlodawska, Marta AU - Mlodawska M AUID- ORCID: 0000-0001-8867-7638 AD - Institute of Medical Sciences, Collegium Medicum, Jan Kochanowski University in Kielce, 25-516 Kielce, Poland. FAU - Swiercz, Grzegorz AU - Swiercz G AD - Institute of Medical Sciences, Collegium Medicum, Jan Kochanowski University in Kielce, 25-516 Kielce, Poland. AD - Clinic of Obstetrics and Gynecology, Provincial Combined Hospital in Kielce, 25-736 Kielce, Poland. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13702384 OTO - NOTNLM OT - FetalHQ OT - fetal echocardiography OT - fetal speckle-tracking echocardiography OT - minimal detectable change OT - observer variation OT - reproducibility of results OT - threshold instability COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:35 PHST- 2026/09/05 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - biomedicines14102392 [pii] AID - biomedicines-14-02392 [pii] AID - 10.3390/biomedicines14102392 [doi] PST - epublish SO - Biomedicines. 2026 Oct 19;14(10):2392. doi: 10.3390/biomedicines14102392. PMID- 42940196 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 19 TI - Antibody Targeting Soluble NKG2D Ligand MIC Enhances Adoptive T-Cell Therapy in an NK Cell-Dependent Mechanism. LID - 10.3390/biomedicines14102390 [doi] LID - 2390 AB - Background/Objectives: Antigen-specific adoptive CD8(+) T-cell therapy has demonstrated promising clinical activity in selected cancers; however, its broader efficacy against solid tumors remains limited by the immunosuppressive tumor microenvironment (TME), which compromises the persistence and function of transferred T-cells. A clinically relevant component of the human solid-tumor TME is soluble MHC class I chain-related molecules A and B (sMICA and sMICB, collectively termed sMIC), which are tumor-derived soluble NKG2D ligands that are not naturally expressed in mice. sMIC promotes tumor immune evasion through multiple mechanisms, but whether therapeutic clearance of sMIC can enhance the efficacy of adoptive T-cell therapy remains unclear. Methods: B16F10 melanoma cells engineered to express soluble MICB (B16F10-sMICB) were implanted subcutaneously into syngeneic B6/MICB mice. Once tumors became palpable, mice were randomized to four treatment groups: (1) no treatment; (2) adoptive transfer of gp100-specific Pmel CD8(+)T cells; (3) the sMIC-targeting monoclonal antibody B10G5; or (4) B10G5 combined with Pmel adoptive cell transfer. Pmel CD8(+)T cells were administered once intravenously, and B10G5 was administered intraperitoneally twice weekly at approximately 3 mg/kg. Tumor growth and body weight were monitored twice weekly. To determine the contribution of natural killer (NK) cells to the therapeutic effect of the combination treatment, NK cells were depleted using the anti-NK1.1 antibody PK136. The impact of NK cells on CD8(+)T cell function during B10G5/sMIC stimulation was further evaluated ex vivo. Results: Therapeutic targeting of sMIC with B10G5 significantly enhanced the antitumor efficacy of adoptively transferred Pmel CD8(+)T cells and was associated with increased donor T-cell persistence, tumor infiltration, and antigen-specific effector function. NK cell depletion markedly diminished the antitumor efficacy of the B10G5/Pmel combination and reduced the abundance, activation, and effector function of donor CD8(+) T-cells. Consistent with these in vivo findings, ex vivo depletion of NK cells impaired IFN-gamma production by NKG2D(+)CD8(+) T-cells during B10G5/sMIC stimulation, supporting a functional role for NK cells in sustaining CD8(+)T cell responses. Conclusions: These findings provide evidence that therapeutic targeting of tumor-derived sMIC can enhance the efficacy of antigen-specific adoptive CD8(+)T cell therapy by promoting the persistence and function of transferred T-cells. Importantly, our results identify NK cells as critical contributors to the enhanced therapeutic response, revealing functional cooperation between NK cells and adoptively transferred CD8(+)T cells during sMIC-targeted therapy. Together, these findings provide preclinical proof-of-concept for combining sMIC-targeting antibodies with adoptive T-cell therapy to improve antitumor immunity against sMIC(+) solid tumors. FAU - Basher, Fahmin AU - Basher F AUID- ORCID: 0000-0002-6368-6762 AD - Division of Hematologic Malignancies and Cellular Therapy, Department of Medicine, Duke University School of Medicine, Durham, NC 27710, USA. AD - Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29425, USA. FAU - Smith, Tyler A AU - Smith TA AUID- ORCID: 0000-0002-3371-0880 AD - Department of Urology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. FAU - Rubinstein, Mark P AU - Rubinstein MP AD - Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29425, USA. AD - Division of Medical Oncology, Department of Internal Medicine, The Ohio State University, Columbus, OH 43210, USA. AD - Pelotonia Institute for Immuno-Oncology, The Ohio State University, Columbus, OH 43210, USA. FAU - Wu, Jennifer D AU - Wu JD AUID- ORCID: 0000-0002-8925-268X AD - Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29425, USA. AD - Department of Urology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. LA - eng GR - 5683-25/Blood Cancer United/ GR - P50 CA180995/CA/NCI NIH HHS/United States GR - R01 CA284640/CA/NCI NIH HHS/United States GR - W81XWH 21-1-0574/USARMC Award/ GR - T32 CA070085/CA/NCI NIH HHS/United States GR - P50CA180995/SPORE/ GR - T32 AI007476/AI/NIAID NIH HHS/United States GR - 5T32AI007476/NIH/NIAID training/ GR - R01 CA290719/CA/NCI NIH HHS/United States GR - R01CA290719/NIH/NCI/ GR - R01CA284640/NIH/NCI/ GR - T32CA070085/NIH/NCI training/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13702291 OTO - NOTNLM OT - NK cells OT - NKG2D OT - adoptive T cell therapy OT - antibody OT - soluble MIC (sMIC) COIS- J.D.W. is the inventor of the B10G5 antibody and has granted international patents. All other authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:35 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - biomedicines14102390 [pii] AID - biomedicines-14-02390 [pii] AID - 10.3390/biomedicines14102390 [doi] PST - epublish SO - Biomedicines. 2026 Oct 19;14(10):2390. doi: 10.3390/biomedicines14102390. PMID- 42940193 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 19 TI - Genetic Susceptibility Within the Immune System Beyond Conventional Risk Factors: An Integrative Analysis in a Kazakh Cohort. LID - 10.3390/biomedicines14102387 [doi] LID - 2387 AB - Background/Objectives: Genetic factors are known to influence overall immune vulnerability to diseases, such as tuberculosis (TB); however, isolating the genetic component can be challenging as social and environmental causes, such as smoking, alcohol consumption, and unemployment, also drive disease risk. To reduce heterogeneity related to these factors, we performed a targeted immunogenetic association study of TB susceptibility in a Kazakh case-control cohort selected among individuals without the typical socioeconomic and nutritional risk factors linked to TB. Methods: We performed genotyping using an immune-focused targeted array; after quality control at sample and variant level, removal of ancestry-based outliers, and adjustment for population stratification, the final dataset included 164 participants (84 cases, 80 controls) and 181,595 variants. We performed four complementary analyses: single-variant association testing using logistic regression with Firth correction where required adjusted for sex and genetic ancestry, gene-based aggregation testing, functional annotation, and polygenic risk scoring using existing multi-ancestry TB genetic resources. Results: While no variant reached formal significance, the top nominal signal, rs1560036 (OR = 2.98, p = 2.4 x 10(-5)), lies within CPLX2, which ranked among the top genes in gene-based analysis, a result driven largely by the same variant. A nominally associated variant in the classical MHC region, rs9263600, is separately reported in GTEx as a cis-eQTL for HLA-B and HLA-C. Similarly, polygenic risk scores showed directionally consistent but statistically non-significant associations with case status (Asia-base AUC = 0.560; Europe-base AUC = 0.537). Conclusions: Although our study was not adequately powered to detect small-to-moderate genetic effects, it offers an important perspective on TB genetics in a population underrepresented in existing research. These findings provide an openly available dataset and candidate signals for replication in larger, independent cohorts. FAU - Serikzhan, Aygerim AU - Serikzhan A AUID- ORCID: 0009-0004-5577-6540 AD - Center for Life Sciences, National Laboratory Astana, Nazarbayev University, Astana 010000, Kazakhstan. FAU - Abilova, Zhannur AU - Abilova Z AD - Center for Life Sciences, National Laboratory Astana, Nazarbayev University, Astana 010000, Kazakhstan. FAU - Akhmetova, Ainur AU - Akhmetova A AD - Center for Life Sciences, National Laboratory Astana, Nazarbayev University, Astana 010000, Kazakhstan. FAU - Gabdulkayum, Aidana AU - Gabdulkayum A AUID- ORCID: 0000-0003-1551-3637 AD - Center for Life Sciences, National Laboratory Astana, Nazarbayev University, Astana 010000, Kazakhstan. FAU - Daniyarov, Asset Z AU - Daniyarov AZ AUID- ORCID: 0000-0003-3886-718X AD - Center for Life Sciences, National Laboratory Astana, Nazarbayev University, Astana 010000, Kazakhstan. AD - Department of General Biology and Genomics, Institute of Natural Sciences, L.N. Gumilyov Eurasian National University, Astana 010000, Kazakhstan. FAU - Rakhimova, Saule E AU - Rakhimova SE AUID- ORCID: 0000-0002-8245-2400 AD - Center for Life Sciences, National Laboratory Astana, Nazarbayev University, Astana 010000, Kazakhstan. FAU - Kozhamkulov, Ulan AU - Kozhamkulov U AD - Center for Life Sciences, National Laboratory Astana, Nazarbayev University, Astana 010000, Kazakhstan. FAU - Kairov, Ulykbek AU - Kairov U AUID- ORCID: 0000-0001-8511-8064 AD - Center for Life Sciences, National Laboratory Astana, Nazarbayev University, Astana 010000, Kazakhstan. FAU - Akilzhanova, Ainur AU - Akilzhanova A AUID- ORCID: 0000-0001-6161-8355 AD - Center for Life Sciences, National Laboratory Astana, Nazarbayev University, Astana 010000, Kazakhstan. FAU - Yerezhepov, Dauren AU - Yerezhepov D AUID- ORCID: 0000-0002-4161-1348 AD - Center for Life Sciences, National Laboratory Astana, Nazarbayev University, Astana 010000, Kazakhstan. LA - eng GR - BR27199879/Science Committee of the Ministry of Science and Higher Education of the Republic of Kazakhstan/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13702402 OTO - NOTNLM OT - Kazakh population OT - genetic association study OT - immunogenetic array OT - polygenic risk score OT - tuberculosis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:35 PHST- 2026/09/22 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - biomedicines14102387 [pii] AID - biomedicines-14-02387 [pii] AID - 10.3390/biomedicines14102387 [doi] PST - epublish SO - Biomedicines. 2026 Oct 19;14(10):2387. doi: 10.3390/biomedicines14102387. PMID- 42940190 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 19 TI - Heart Transplantation: A Review of Recent Advancements in Pre-, Peri, and Post-Transplant Screening and Management. LID - 10.3390/biomedicines14102384 [doi] LID - 2384 AB - Heart transplantation (HT) remains a critical treatment for patients with end-stage heart failure who remain unresponsive to optimized guideline-directed medical therapy. This review examines recent advancements in pre-, peri-, and post-transplant screening and management aimed at improving patient selection, transplant accessibility, and clinical outcomes. Pre-transplant strategies include comprehensive HT candidate evaluation, risk stratification, donor selection, allocation policies that priotize critically ill patients, and mechanical circulatory support (MCS) to stabilize patients awaiting transplantation. Peri-transplant advances focus on strategies to maintain hemodynamic stability, minimize ischemic injury, and maintain graft viability immediately after HT. Post-transplant management emphasizes individualized immunosuppressive regimens, cardiac allograft rejection surveillance, and the prevention and management of complications to improve graft survival and long-term patient outcomes. Despite substantial progress, significant challenges remain, particularly the shortage of suitable donor organs and the risk of immune-mediated graft rejection. Emerging approaches, including artificial intelligence, xenotransplantation, and regenerative medicine, offer promising opportunities to expand the donor pool, enhance clinical decision-making, and improve HT outcomes. This review examines the research that influences the current policies and practices involved in HT, featuring the pivotal findings as well as any shortcomings present among these studies. Ultimately, continued research and multidisciplinary collaboration in each phase of HT are essential to improve the short and long-term health outcomes of transplant candidates. By integrating established evidence-based practices with emerging technologies, clinicians may further improve equitable access, survival, and quality-of-life for patients with end-stage heart failure. FAU - Ringor, Marc AU - Ringor M AD - Department of Internal Medicine, University of Nevada, Las Vegas, NV 89102, USA. FAU - Nguyen, Darren AU - Nguyen D AUID- ORCID: 0009-0006-3330-7510 AD - Department of Internal Medicine, University of Nevada, Las Vegas, NV 89102, USA. FAU - Boumelhem, Fadel AU - Boumelhem F AD - Department of Internal Medicine, University of Nevada, Las Vegas, NV 89102, USA. FAU - Joseph, Yousef AU - Joseph Y AD - Department of Internal Medicine, University of Nevada, Las Vegas, NV 89102, USA. FAU - Sharma, Divyansh AU - Sharma D AUID- ORCID: 0009-0006-8966-4189 AD - Department of Internal Medicine, University of Nevada, Las Vegas, NV 89102, USA. FAU - Dugal, Jasmine AU - Dugal J AD - Department of Internal Medicine, University of Nevada, Las Vegas, NV 89102, USA. LA - eng GR - University of Nevada, Las Vegas/ PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13702431 OTO - NOTNLM OT - cardiac allograft rejection OT - echocardiography OT - end-stage heart failure OT - heart transplantation OT - immunosuppressive therapy OT - induction therapy OT - left ventricular assist device OT - maintenance therapy OT - mechanical circulatory support OT - surveillance COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:35 PHST- 2026/08/24 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - biomedicines14102384 [pii] AID - biomedicines-14-02384 [pii] AID - 10.3390/biomedicines14102384 [doi] PST - epublish SO - Biomedicines. 2026 Oct 19;14(10):2384. doi: 10.3390/biomedicines14102384. PMID- 42940181 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 18 TI - Stage-Stratified Explainable Machine Learning Identifies a Reproducible Transcriptomic Classifier with Stage-Dependent Feature Importance in Colorectal Cancer: A Multi-Cohort Study with Saudi Population Contextualisation. LID - 10.3390/biomedicines14102375 [doi] LID - 2375 AB - Background: Colorectal cancer (CRC) is molecularly heterogeneous across stages, yet most transcriptomic classifiers treat it as static and rarely test whether the importance of their genes changes with disease stage. Methods: A stage-stratified explainable machine-learning pipeline was applied to the Cancer Genome Atlas colon adenocarcinoma cohort (286 tumour, 41 normal). A ten-gene panel was derived by cross-validated stability selection; six classifiers were benchmarked under a leakage-free protocol; and exact SHapley Additive exPlanations (SHAP) were computed within each American Joint Committee on Cancer (AJCC) stage. Findings were tested in three independent cohorts (GSE50760, GSE39582, GSE17536) and corroborated by literature, GEPIA2, and four-platform pathway enrichment. Results: The ten-gene panel classified tumour versus normal tissue with internal AUC up to 1.000 and external AUC 0.969 (95% CI 0.896-1.000; area under the precision-recall curve [AUPRC] 0.980), robust to class imbalance. Stage-stratified SHAP showed a monotonic shift in feature importance across stages (cross-stage Spearman rho 0.66-0.93) that replicated at three of four stages in an independent cohort (rho up to 0.94); ESM1 rose to top-two late-stage importance in both cohorts, consistent with its role in CRC angiogenesis. The panel recovered known cancer biology, nominated novel candidates, and proved complementary to network-hub genes, and its enriched pathways matched four of five frequently altered Saudi CRC pathways. Survival discrimination was at chance (C-index 0.50-0.58), defining the panel as a classifier rather than a prognostic model. Conclusions: This is, to our knowledge, the first evidence in CRC that an explainable classifier's feature-importance structure is itself stage-dependent and externally replicable. Coupling exact interpretability with reproducible, transparently scoped validation, the panel generalises as a classifier but not a prognostic model, and its convergence with Saudi CRC alterations defines conditions for prospective population-specific validation. Code and processed data are publicly available. FAU - Syed, Asif Hassan AU - Syed AH AUID- ORCID: 0000-0002-7288-3098 AD - Department of Computer Science, Faculty of Computing and Information Technology in Rabigh, King Abdulaziz University, Jeddah 21589, Saudi Arabia. FAU - Alhayyani, Sultan AU - Alhayyani S AUID- ORCID: 0000-0002-6529-9299 AD - Department of Chemistry, Faculty of Sciences and Arts in Rabigh, King Abdulaziz University, Jeddah 21589, Saudi Arabia. FAU - Alromema, Nashwan AU - Alromema N AUID- ORCID: 0000-0001-6208-2863 AD - Department of Computer Science, Faculty of Computing and Information Technology in Rabigh, King Abdulaziz University, Jeddah 21589, Saudi Arabia. FAU - A Almazarqi, Hatem AU - A Almazarqi H AUID- ORCID: 0000-0001-6644-493X AD - Department of Information Technology, Faculty of Computing and Information Technology in Rabigh, King Abdulaziz University, Jeddah 21911, Saudi Arabia. FAU - Taha, Altyeb Altaher AU - Taha AA AUID- ORCID: 0000-0001-9086-3085 AD - Department of Information Technology, Faculty of Computing and Information Technology in Rabigh, King Abdulaziz University, Jeddah 21911, Saudi Arabia. LA - eng GR - IPP: 66-830-2026/King Abdulaziz University/ PT - Journal Article DEP - 20261018 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701872 OTO - NOTNLM OT - ESM1 OT - SHapley Additive exPlanations OT - colorectal cancer OT - differential expression OT - explainable artificial intelligence OT - external validation OT - feature importance OT - machine learning OT - stage stratification OT - transcriptomics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:35 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - biomedicines14102375 [pii] AID - biomedicines-14-02375 [pii] AID - 10.3390/biomedicines14102375 [doi] PST - epublish SO - Biomedicines. 2026 Oct 18;14(10):2375. doi: 10.3390/biomedicines14102375. PMID- 42940166 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 16 TI - Donor-Resolved Analysis of HES1-Associated Fibroblast States in Systemic Sclerosis. LID - 10.3390/biomedicines14102360 [doi] LID - 2360 AB - Background/Objectives: We examined HES1-associated fibroblast states in systemic sclerosis (SSc) and the rationale for testing upstream gamma-secretase inhibition with nirogacestat. Methods: We analysed 325,636 skin cells from 230 donors across five non-redundant cohorts using complete computational transcription-factor screens, donor-level activity and trajectory analyses, multiome data and spatial assays. Donors or independent simulations were the inference units, with cohort adjustment and multiplicity correction where applicable. Three independent 200 ns molecular dynamics runs per condition compared nirogacestat-bound and ligand-deleted gamma-secretase models. Results: HES1 activity was positively associated with model-derived myofibroblast fate in 98.1% of 206 donors (median Spearman's rho=0.282; 95% confidence interval [CI], 0.258-0.309). The adjusted SSc-healthy control activity difference was uncertain (0.21; 95% CI, -0.01 to 0.43; Benjamini-Hochberg q=0.061). HES1 ranked 28/107 and 30/109 in the descriptive screens; SMAD3 showed a stronger association with cell fate. Disease-specific communication and contact-enrichment tests did not provide statistical support. The adjusted Visium association was negative; Xenium showed a weak positive mean without section-level corrected support. Nirogacestat was selected retrospectively through mechanistic and translational evidence despite a weak expression-reversal signal and discordant tofacitinib-signature alignment. Simulations showed lower reference-defined pocket root mean square deviation (RMSD) in bound models, with substantial sampling and endpoint-energy uncertainty. Conclusions: HES1 activity marks a consistent fibroblast-state association, with uncertain causality and SSc specificity. Nirogacestat is a candidate compound for experimental testing of upstream Notch-pathway perturbation in SSc fibroblasts; the computational findings do not establish efficacy. FAU - Fang, Shenyi AU - Fang S AUID- ORCID: 0009-0005-7884-9666 AD - The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310053, China. FAU - Huang, Lin AU - Huang L AUID- ORCID: 0000-0002-8036-8408 AD - Innovation Center for Medical Basic Research of Autoimmune Diseases, China National Ministry of Education, College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China. FAU - Wang, Mingzhu AU - Wang M AD - Innovation Center for Medical Basic Research of Autoimmune Diseases, China National Ministry of Education, College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou 310053, China. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13702352 OTO - NOTNLM OT - HES1 OT - Notch OT - drug repurposing OT - gene-regulatory network OT - myofibroblast OT - nirogacestat OT - systemic sclerosis OT - virtual perturbation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:35 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - biomedicines14102360 [pii] AID - biomedicines-14-02360 [pii] AID - 10.3390/biomedicines14102360 [doi] PST - epublish SO - Biomedicines. 2026 Oct 16;14(10):2360. doi: 10.3390/biomedicines14102360. PMID- 42940164 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 16 TI - Metabolically Unhealthy Obesity: Novel Subtypes Defined by Integrated Metabolomics and Hematology. LID - 10.3390/biomedicines14102358 [doi] LID - 2358 AB - Objectives: The aim was to identify metabolic subtypes within metabolically unhealthy obesity (MUO) using integrated metabolomics and hematology data and evaluate their differential risks for cardiovascular-kidney-metabolic (CKM)-related outcomes. Methods: Among 62,341 obese UK Biobank participants (43,577 MUO; 18,764 non-MUO), least absolute shrinkage and selection operator regression selected 67 biomarkers from 96 metabolites and serological parameters, followed by K-means clustering. Cox proportional hazards models assessed subtype-specific associations with incident CKM-related outcomes over a median 14.81-year follow-up. Results: Four metabolic subtypes were identified within MUO, characterized by distinct amino acid, renal, and lipid profiles. Subtype-specific associations with CKM-related outcomes differed substantially. Compared with non-MUO participants, Subtype II and Subtype IV were associated with higher coronary heart disease risk (HR, 1.34; 95% CI, 1.11-1.61 and HR, 1.37; 95% CI, 1.15-1.64, respectively), Subtype IV was associated with lower atrial fibrillation risk (HR, 0.73; 95% CI, 0.60-0.90), Subtype III with higher heart failure risk (HR, 1.42; 95% CI, 1.10-1.82), and Subtype I with higher chronic kidney disease risk (HR, 1.52; 95% CI, 1.22-1.91). Conclusions: Integrated metabolomic and hematologic profiling identified variation in the metabolic profiles of individuals with MUO. The four subtypes showed different patterns of association with CKM-related outcomes, suggesting that MUO encompasses diverse metabolic profiles that may not be fully captured by conventional metabolic syndrome criteria. FAU - Huang, Xuan AU - Huang X AD - Department of Cardiology, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou University Affiliated Provincial Hospital, Fuzhou 355000, China. AD - Fujian Provincial Key Laboratory of Cardiovascular Disease, Fujian Provincial Center for Geriatrics, Fujian Provincial Clinical Research Center for Severe Acute Cardiovascular Diseases, Fuzhou 355000, China. AD - Fujian Heart Failure Center Alliance, Fuzhou 355000, China. FAU - Liu, Yuhong AU - Liu Y AD - Department of Cardiology, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou University Affiliated Provincial Hospital, Fuzhou 355000, China. AD - Fujian Provincial Key Laboratory of Cardiovascular Disease, Fujian Provincial Center for Geriatrics, Fujian Provincial Clinical Research Center for Severe Acute Cardiovascular Diseases, Fuzhou 355000, China. AD - Fujian Heart Failure Center Alliance, Fuzhou 355000, China. FAU - Zhu, Xiaoqian AU - Zhu X AD - Department of Cardiology, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou University Affiliated Provincial Hospital, Fuzhou 355000, China. AD - Fujian Provincial Key Laboratory of Cardiovascular Disease, Fujian Provincial Center for Geriatrics, Fujian Provincial Clinical Research Center for Severe Acute Cardiovascular Diseases, Fuzhou 355000, China. AD - Fujian Heart Failure Center Alliance, Fuzhou 355000, China. FAU - Lin, Zhennan AU - Lin Z AD - Department of Cardiology, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou University Affiliated Provincial Hospital, Fuzhou 355000, China. AD - Fujian Provincial Key Laboratory of Cardiovascular Disease, Fujian Provincial Center for Geriatrics, Fujian Provincial Clinical Research Center for Severe Acute Cardiovascular Diseases, Fuzhou 355000, China. AD - Fujian Heart Failure Center Alliance, Fuzhou 355000, China. AD - Center for Cardiovascular Epidemiology Research and Prevention of Fujian Provincial Hospital, Fuzhou 355000, China. FAU - Guo, Yansong AU - Guo Y AUID- ORCID: 0000-0001-6607-6938 AD - Department of Cardiology, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou University Affiliated Provincial Hospital, Fuzhou 355000, China. AD - Fujian Provincial Key Laboratory of Cardiovascular Disease, Fujian Provincial Center for Geriatrics, Fujian Provincial Clinical Research Center for Severe Acute Cardiovascular Diseases, Fuzhou 355000, China. AD - Fujian Heart Failure Center Alliance, Fuzhou 355000, China. FAU - Lin, Kaiyang AU - Lin K AUID- ORCID: 0000-0003-1421-2728 AD - Department of Cardiology, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou University Affiliated Provincial Hospital, Fuzhou 355000, China. AD - Fujian Provincial Key Laboratory of Cardiovascular Disease, Fujian Provincial Center for Geriatrics, Fujian Provincial Clinical Research Center for Severe Acute Cardiovascular Diseases, Fuzhou 355000, China. AD - Fujian Heart Failure Center Alliance, Fuzhou 355000, China. LA - eng GR - 2023Y9321/Fujian Provincial Finance Department/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701604 OTO - NOTNLM OT - cardiovascular-kidney-metabolic syndrome OT - metabolically unhealthy obesity OT - metabolomics OT - risk stratification COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:35 PHST- 2026/08/28 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - biomedicines14102358 [pii] AID - biomedicines-14-02358 [pii] AID - 10.3390/biomedicines14102358 [doi] PST - epublish SO - Biomedicines. 2026 Oct 16;14(10):2358. doi: 10.3390/biomedicines14102358. PMID- 42940163 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 16 TI - Baclofen for Spasticity in Multiple Sclerosis: A Comprehensive Narrative Review. LID - 10.3390/biomedicines14102357 [doi] LID - 2357 AB - Baclofen, a GABA-B receptor agonist developed as an anticonvulsant in the 1960s and repurposed for spasticity in the 1970s, is recommended as a first-line oral treatment for spasticity in multiple sclerosis (MS) by UK NICE guidance and is used similarly, though not necessarily designated identically, in many other jurisdictions. It acts at two sites within the spinal stretch reflex arc: presynaptically on Ia afferent terminals in the dorsal horn, where it reduces excitatory neurotransmitter release, and postsynaptically on ventral horn interneurons and alpha-motoneurons, where it hyperpolarises the cell and suppresses firing. Only a small fraction of an oral dose crosses the blood-CNS barrier, so oral dosing requires high systemic doses that cause sedation, ataxia and CNS depression. Intrathecal delivery bypasses the barrier, allowing a much lower dose and fewer brain-mediated side effects. Other drawbacks include a short, variable half-life, GABA-B receptor downregulation contributing to adverse effects and intrathecal tolerance, and withdrawal that has proved fatal. The narrow efficacy-to-side-effect margin is probably intrinsic to GABA-B receptor activation. This narrative review covers baclofen's neuroanatomy, pharmacokinetics, administration routes, efficacy, comparators, long-term outcomes, interactions, special populations, and regulatory history. Long-term follow-up shows durable control without worsening sedation, but the one study using validated quality-of-life measures found no significant improvement despite clear spasticity reduction. Weighing benefit against harm, this review concludes that baclofen offers a net benefit, but conditionally: only for carefully selected, individually titrated, and actively monitored patients. Because this is a narrative rather than systematic review, source selection was not governed by a prespecified protocol, some clinical estimates rely on secondary or aggregate data, and the underlying literature is heterogeneous in design and quality. FAU - Palmer, Alan M AU - Palmer AM AUID- ORCID: 0000-0002-2819-6362 AD - Elixa MediScience Ltd., 24-28 Brockenhurst Road, Ascot, Berkshire SL5 9DL, UK. LA - eng PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701932 OTO - NOTNLM OT - GABA-B OT - baclofen OT - downregulation OT - injections OT - multiple sclerosis OT - muscle spasticity OT - pharmacokinetics OT - receptors OT - spinal OT - substance withdrawal syndrome COIS- A.M.P. is a director of Elixa MediScience Ltd., which is developing neuroprotective compounds for neurodegenerative disorders; these compounds act through mechanisms unrelated to GABA-B receptor signalling and are not competitors of, or complementary products to, baclofen or any other agent discussed in this review. The author has no other competing interests to declare. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:35 PHST- 2026/09/21 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - biomedicines14102357 [pii] AID - biomedicines-14-02357 [pii] AID - 10.3390/biomedicines14102357 [doi] PST - epublish SO - Biomedicines. 2026 Oct 16;14(10):2357. doi: 10.3390/biomedicines14102357. PMID- 42940162 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 16 TI - Integrated Bile Metabolomics and Serum Biomarkers for Differentiating Cholangiocarcinoma from Cholelithiasis. LID - 10.3390/biomedicines14102356 [doi] LID - 2356 AB - Background: We investigated bile metabolites and serum variables for differentiating cholangiocarcinoma (CCA) from cholelithiasis. Methods: Bile from 31 patients with CCA and 48 with cholelithiasis was profiled by high-resolution LC-MS. Candidate metabolites and serum variables were integrated in a seven-variable classifier, and public single-cell data provided tissue-level biological context. Results: Of 2269 annotated metabolite features, 532 met the exploratory screening criteria. The selected signature comprised pyruvic acid, lipoxin A4, CEA, APOA1, 3-chloro-L-tyrosine, ADP-ribose, and glutaric acid; pyruvic acid had the largest contribution in the SHAP analysis. Random forest achieved an AUC of 0.894 in the classifier comparison, a training cross-validation AUC of 0.820, and an AUC of 0.914 in the 16-patient test subset. Because selection used all 79 patients before evaluation, these internal performance estimates may be optimistic. Conclusions: Integrated bile and serum profiling identified a promising seven-variable signature for differentiating CCA from cholelithiasis in this cohort. Added diagnostic value over routine serum markers and performance in other benign biliary conditions have not been established. FAU - Zhang, Jinduo AU - Zhang J AD - Department of General Surgery, The First Hospital of Lanzhou University, No. 1 Donggang West Road, Lanzhou 730030, China. FAU - Wang, Leiqing AU - Wang L AD - The First School of Clinical Medicine, Lanzhou University, Lanzhou 730030, China. FAU - Zhang, Xu AU - Zhang X AD - Endoscopic Diagnosis and Treatment Center, Gansu Provincial Hospital, Lanzhou 730000, China. FAU - Yue, Ping AU - Yue P AD - Department of General Surgery, The First Hospital of Lanzhou University, No. 1 Donggang West Road, Lanzhou 730030, China. FAU - Jin, Boru AU - Jin B AD - The First School of Clinical Medicine, Lanzhou University, Lanzhou 730030, China. FAU - Mi, Ningning AU - Mi N AD - Clinical Research Center, Big Data Center, The Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 518107, China. FAU - Zhao, Jinyu AU - Zhao J AD - The First School of Clinical Medicine, Lanzhou University, Lanzhou 730030, China. FAU - Gao, Long AU - Gao L AD - Department of General Surgery, The First Hospital of Lanzhou University, No. 1 Donggang West Road, Lanzhou 730030, China. FAU - Lee, Dylan AU - Lee D AD - CuraStrategix Health, Aurora, OH 44202, USA. FAU - Lin, Yanyan AU - Lin Y AD - Department of General Surgery, The First Hospital of Lanzhou University, No. 1 Donggang West Road, Lanzhou 730030, China. FAU - Meng, Wenbo AU - Meng W AD - Department of General Surgery, The First Hospital of Lanzhou University, No. 1 Donggang West Road, Lanzhou 730030, China. AD - Gansu Province Key Laboratory of Biological Therapy and Regenerative Medicine Transformation, Lanzhou 730030, China. LA - eng GR - 2022YFC24074/National Key Research and Development Program of China/ GR - lzujbky-2025-it42/Fundamental Research Funds for the Central Universities/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701907 OTO - NOTNLM OT - bile metabolomics OT - cholangiocarcinoma OT - cholelithiasis OT - diagnostic model OT - exploratory analysis OT - pyruvate COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:35 PHST- 2026/08/25 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - biomedicines14102356 [pii] AID - biomedicines-14-02356 [pii] AID - 10.3390/biomedicines14102356 [doi] PST - epublish SO - Biomedicines. 2026 Oct 16;14(10):2356. doi: 10.3390/biomedicines14102356. PMID- 42940160 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 16 TI - Circulating Zonulin in Irritable Bowel Syndrome: A Systematic Review of Conflicting Evidence and the Critical Impact of Assay Specificity. LID - 10.3390/biomedicines14102354 [doi] LID - 2354 AB - Background: Increased intestinal permeability is a proposed pathophysiological mechanism in irritable bowel syndrome (IBS), and zonulin, a putative regulator of tight junctions, has been investigated as a non-invasive serum biomarker. However, the diagnostic utility of circulating zonulin in IBS remains controversial. Objectives: To systematically review studies comparing serum or plasma zonulin concentrations between adult IBS patients and healthy controls, and to evaluate the influence of assay methodology on reported outcomes. Methods: A systematic literature search of PubMed was performed up to April 2026; the most recent eligible study was published in June 2024. Studies that enrolled adults with IBS diagnosed by Rome criteria, included healthy control groups, and measured zonulin by enzyme-linked immunosorbent assay (ELISA) were eligible. Studies lacking a healthy control group, non-original reports, animal studies, and mixed functional gastrointestinal disorder cohorts without extractable IBS data were excluded. Findings were synthesised narratively due to substantial methodological heterogeneity and concerns regarding the validity of commercially available zonulin kits. Risk of bias was assessed using the Newcastle-Ottawa Scale for case-control studies; six studies were at low risk and two at intermediate risk. The review is reported in accordance with the PRISMA 2020 statement, and this abstract follows the PRISMA 2020 for Abstracts checklist. Results: Eight case-control studies were included, comprising 421 IBS patients and 428 controls. Of the seven studies contributing to the primary synthesis, two reported significantly elevated zonulin in IBS, one produced opposite results with two different assays applied to the same samples, and four found no significant difference compared with controls. An eighth study, which combined IBS with inflammatory bowel disease and did not report IBS-specific data, was retained as supportive, non-primary evidence. Crucially, the studies employed different ELISA kits (ALPCO, Cusabio, Immundiagnostik, Zellbio) and different units of measurement. Independent validation studies have demonstrated that the most frequently used commercial kits do not recognise the pre-haptoglobin-2 molecule claimed to be zonulin, but instead detect haptoglobin, complement C3, or properdin. The only study employing a monoclonal antibody specific for pre-haptoglobin-2 observed lower, rather than higher, concentrations in IBS. Conclusions: The existing literature based on commercial ELISAs cannot determine whether pre-HP2/zonulin is altered in IBS, and the single genotype-controlled monoclonal assay suggests lower, not higher, concentrations in IBS. The disparate results are largely attributable to the application of ELISA kits that lack analytical specificity for the proposed target. Future research must use analytically validated assays, such as monoclonal antibody-based methods with concomitant genotyping for haptoglobin polymorphisms, before zonulin can be considered a meaningful biomarker in IBS. Funding: The Article Processing Charges were funded by the University of Medicine and Pharmacy of Craiova, Romania. Registration: The review was not prospectively registered; a retrospective protocol registration is available on OSF [10.17605/OSF.IO/MFA6J]. FAU - Marginean, Iulia Cristina AU - Marginean IC AUID- ORCID: 0009-0005-3968-255X AD - Doctoral School, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Marginean, Cristina Maria AU - Marginean CM AUID- ORCID: 0009-0002-9956-4318 AD - Department of Internal Medicine, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Cazacu, Sergiu Marian AU - Cazacu SM AUID- ORCID: 0000-0001-9623-7683 AD - Research Center of Gastroenterology and Hepatology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Iacob, George Alexandru AU - Iacob GA AUID- ORCID: 0009-0000-4783-7420 AD - Department of Radiology and Medical Imaging, Clinical Emergency County Hospital Craiova, 200349 Craiova, Romania. FAU - Fortofoiu, Dragos AU - Fortofoiu D AUID- ORCID: 0009-0002-4143-844X AD - Doctoral School, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Otoiu, Marian Catalin AU - Otoiu MC AD - Doctoral School, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Popescu, Mihaela AU - Popescu M AD - Department of Endocrinology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. FAU - Vere, Cristin Constantin AU - Vere CC AD - Research Center of Gastroenterology and Hepatology, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania. LA - eng GR - University of Medicine and Pharmacy of Craiova/ PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13702017 OTO - NOTNLM OT - ELISA OT - biomarker OT - intestinal permeability OT - irritable bowel syndrome OT - pre-haptoglobin-2 OT - zonulin COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:35 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - biomedicines14102354 [pii] AID - biomedicines-14-02354 [pii] AID - 10.3390/biomedicines14102354 [doi] PST - epublish SO - Biomedicines. 2026 Oct 16;14(10):2354. doi: 10.3390/biomedicines14102354. PMID- 42940157 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 15 TI - Postprocessing of Structural En Face OCT Images of the Choroidal Haller Layer for Quantitative Assessment: An Exploratory Pilot Study. LID - 10.3390/biomedicines14102351 [doi] LID - 2351 AB - Background/Objectives: To develop a binarization workflow for structural en face OCT images of the choroidal Haller layer and examine how postprocessing parameters affect measurements, using chronological age as an exploratory reference. Methods: We included 66 healthy volunteers (66 eyes; 22-73 years; three age groups). En face Haller layer images from swept-source OCT angiography scans (DRI OCT Triton; 6 x 6 mm) were binarized using retinal vessel masking and Phansalkar thresholding. Radius R (22 values) and coefficients k and r (24 combinations) were varied. Results: Measurements depended strongly on R; age-group separation was similar over R = 13-26 pixels; permutation testing gave p = 0.031 across radii and p = 0.115 across all 45 configurations. Using the representative working configurations (R = 17 pixels; default k = 0.25 and r = 0.50), vessel density (VD), vessel length density (VLD), and inter-vessel distance (IVD) showed modest, non-monotonic nominal age-group differences (eta(2) approximately 0.10); those for VD and IVD were lost after accounting for masked regions. Adjusted for sex and spherical equivalent, VLD decreased and IVD increased with age, whereas VD did not. Conclusions: Measurements depend substantially on postprocessing parameters. The working configuration, selected and evaluated in one cohort, requires repeatability testing and independent validation. FAU - Khalatyan, Anait S AU - Khalatyan AS AUID- ORCID: 0000-0001-6255-5544 AD - Krasnov Research Institute of Eye Diseases, Moscow 119021, Russia. FAU - Yusef, Yusef AU - Yusef Y AD - Krasnov Research Institute of Eye Diseases, Moscow 119021, Russia. FAU - Avetisov, Konstantin S AU - Avetisov KS AD - Krasnov Research Institute of Eye Diseases, Moscow 119021, Russia. FAU - Smolin, Sergey A AU - Smolin SA AUID- ORCID: 0000-0003-2226-0594 AD - Krasnov Research Institute of Eye Diseases, Moscow 119021, Russia. AD - Department of Ophthalmology, Sechenov First Moscow State Medical University (Sechenov University), Moscow 119991, Russia. FAU - Siplivy, Vladimir I AU - Siplivy VI AD - Department of Ophthalmology, Sechenov First Moscow State Medical University (Sechenov University), Moscow 119991, Russia. FAU - Aslamazova, Anna E AU - Aslamazova AE AD - Department of Ophthalmology, Sechenov First Moscow State Medical University (Sechenov University), Moscow 119991, Russia. FAU - Reznikova, Ludmila V AU - Reznikova LV AD - Department of Ophthalmology, Sechenov First Moscow State Medical University (Sechenov University), Moscow 119991, Russia. FAU - Machekhina, Liubov V AU - Machekhina LV AUID- ORCID: 0000-0002-2028-3939 AD - Russian Gerontology Research and Clinical Centre, Pirogov National Research Medical University, Moscow 129226, Russia. FAU - Melnitskaia, Alexandra A AU - Melnitskaia AA AUID- ORCID: 0009-0009-0858-2053 AD - Russian Gerontology Research and Clinical Centre, Pirogov National Research Medical University, Moscow 129226, Russia. FAU - Strazhesko, Irina D AU - Strazhesko ID AD - Russian Gerontology Research and Clinical Centre, Pirogov National Research Medical University, Moscow 129226, Russia. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701795 OTO - NOTNLM OT - Haller layer OT - Phansalkar thresholding OT - aging OT - choroid OT - image postprocessing OT - structural en face imaging OT - swept-source optical coherence tomography COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:35 PHST- 2026/09/24 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - biomedicines14102351 [pii] AID - biomedicines-14-02351 [pii] AID - 10.3390/biomedicines14102351 [doi] PST - epublish SO - Biomedicines. 2026 Oct 15;14(10):2351. doi: 10.3390/biomedicines14102351. PMID- 42940150 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 15 TI - Documented Dry Eye Disease in Patients Receiving Topical Antiglaucoma Therapy: Associations with Treatment Characteristics. LID - 10.3390/biomedicines14102344 [doi] LID - 2344 AB - Background/Objectives: Chronic topical antiglaucoma therapy may affect the ocular surface. We assessed documented dry eye disease (DED) and its associations with active-ingredient burden, treatment duration, and preservative exposure. Methods: This retrospective cross-sectional study included 357 adults receiving topical antiglaucoma therapy at a tertiary center who had complete treatment and ocular-surface data. Ocular-surface testing was prompted by symptoms or clinical judgment. The first eligible visit identified during chronological record review was analyzed for each patient. DED was based on the treating ophthalmologist's documented diagnosis. Logistic regression evaluated treatment-related factors. Results: DED was documented in 171/357 patients within this selected sample (47.9%; 95% CI 42.8-53.1%). Prevalence was higher with at least two versus one active IOP-lowering ingredient (60.6% vs. 23.1%; p < 0.001). Each additional ingredient was associated with higher DED odds after adjustment for age, sex, and glaucoma subtype (adjusted odds ratio [aOR] 2.65, 95% confidence interval [CI] 1.88-3.74). Longer treatment duration was also associated with higher odds (aOR 1.12 per 12 months, 95% CI 1.05-1.20). In the linear extended model, active-ingredient count was not associated with documented DED (aOR 1.02, 95% CI 0.69-1.51), whereas an exclusively preservative-free regimen was associated with lower odds (aOR 0.011, 95% CI 0.001-0.084) and fixed-combination therapy with higher odds (aOR 3.23, 95% CI 1.74-5.98). However, categorical modeling of ingredient count significantly improved extended-model fit (likelihood-ratio p < 0.001), indicating sensitivity to functional form. Documented DED occurred in 1/91 (1.1%) exclusively preservative-free patients versus 170/266 (63.9%) preserved-exposure patients, with sparse PF events limiting interpretation. Conclusions: Documented DED was common in this selected cohort undergoing ocular-surface assessment. Active-ingredient count was associated with DED in the primary adjusted model, but its interpretation in the extended model depended on the functional form used. The marked inverse association with an exclusively preservative-free regimen requires prospective confirmation because sparse data, limited overlap between treatment groups, treatment selection, and residual confounding preclude causal inference. FAU - Jelic Vukovic, Marija AU - Jelic Vukovic M AUID- ORCID: 0000-0002-4450-2657 AD - Department of Ophthalmology, University Hospital Center Osijek, 31 000 Osijek, Croatia. AD - Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, 31 000 Osijek, Croatia. FAU - Kolak, Ena AU - Kolak E AD - Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, 31 000 Osijek, Croatia. FAU - Biuk, Dubravka AU - Biuk D AUID- ORCID: 0000-0002-7269-3534 AD - Department of Ophthalmology, University Hospital Center Osijek, 31 000 Osijek, Croatia. AD - Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, 31 000 Osijek, Croatia. FAU - Susnjara, Petar AU - Susnjara P AUID- ORCID: 0000-0001-9158-2779 AD - Faculty of Kinesiology Osijek, Josip Juraj Strossmayer University of Osijek, 31 000 Osijek, Croatia. FAU - Vidovic, Stipe AU - Vidovic S AUID- ORCID: 0000-0002-3025-6870 AD - Department of Ophthalmology, University Hospital Center Osijek, 31 000 Osijek, Croatia. AD - Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, 31 000 Osijek, Croatia. FAU - Suvaljko, Eleonora AU - Suvaljko E AUID- ORCID: 0000-0001-9068-3084 AD - Department of Ophthalmology, General Hospital Virovitica, 33 000 Virovitica, Croatia. FAU - Peric, Leon AU - Peric L AD - Department of Ophthalmology, University Hospital Center Osijek, 31 000 Osijek, Croatia. AD - Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, 31 000 Osijek, Croatia. FAU - Nemet, Vedran AU - Nemet V AD - Department of Ophthalmology, University Hospital Center Osijek, 31 000 Osijek, Croatia. AD - Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, 31 000 Osijek, Croatia. FAU - Prce, Luka AU - Prce L AD - School of Medicine, University of Zagreb, 10 000 Zagreb, Croatia. FAU - Matic, Suzana AU - Matic S AUID- ORCID: 0000-0002-8040-2784 AD - Department of Ophthalmology, University Hospital Center Osijek, 31 000 Osijek, Croatia. AD - Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, 31 000 Osijek, Croatia. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13702091 OTO - NOTNLM OT - benzalkonium chloride OT - dry eye disease OT - glaucoma OT - medication burden OT - ocular surface disease OT - polypharmacy OT - preservative-free regimen OT - topical antiglaucoma therapy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:35 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - biomedicines14102344 [pii] AID - biomedicines-14-02344 [pii] AID - 10.3390/biomedicines14102344 [doi] PST - epublish SO - Biomedicines. 2026 Oct 15;14(10):2344. doi: 10.3390/biomedicines14102344. PMID- 42940145 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 14 TI - Lipoprotein(a) After Myocardial Infarction: Residual Risk, Measurement, and the Unresolved Therapeutic Question. LID - 10.3390/biomedicines14102339 [doi] LID - 2339 AB - Lipoprotein(a) [Lp(a)] is a highly heritable apoB-containing lipoprotein and a causal contributor to atherosclerotic cardiovascular disease that may contribute to residual cardiovascular risk after myocardial infarction (MI), while the clinical benefit of selective pharmacological Lp(a) lowering remains unresolved. This narrative review examines Lp(a) as a determinant of residual and recurrent cardiovascular risk after MI, integrating molecular biology, human genetics, coronary imaging, post-MI observational studies, measurement considerations, current management, and emerging therapies. Elevated Lp(a) has been associated with coronary plaque progression, high-risk plaque features, oxidized-phospholipid-related inflammatory pathways, recurrent ischemic events, and repeat revascularization, although prognostic associations vary across populations and endpoints. Because concentrations measured during acute MI may underestimate the stable Lp(a) phenotype in some patients, selective repeat measurement after clinical stabilization may be appropriate when classification could influence management or trial eligibility. Contemporary treatment remains centered on intensive control of LDL-C and other modifiable risk factors, while antisense oligonucleotides, small interfering RNAs, and oral particle-assembly inhibitors can produce profound Lp(a) reductions. According to sponsor-reported topline data released in September 2026, pelacarsen lowered Lp(a) but did not significantly reduce the prespecified primary cardiovascular endpoint in the overall Lp(a)HORIZON population; complete peer-reviewed results are not yet available. The sponsor-reported neutral HORIZON topline result raises unresolved questions regarding baseline Lp(a) and absolute reduction, timing of intervention and disease stage, and endpoint composition. Full peer-reviewed HORIZON data and ongoing cardiovascular outcome trials will be required to determine whether and under what conditions selective Lp(a) lowering translates into cardiovascular benefit. FAU - Gogan, Alexandra AU - Gogan A AD - Doctoral School of Medicine, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Sq. No. 2, 300041 Timisoara, Romania. AD - Department of Cardiology, Institute of Cardiovascular Diseases, Str. Gheorghe Adam No. 13A, 300310 Timisoara, Romania. AD - Department V-Internal Medicine 1, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania. FAU - Ardelean, Samuel AU - Ardelean S AUID- ORCID: 0009-0004-8194-4501 AD - Doctoral School of Medicine, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Sq. No. 2, 300041 Timisoara, Romania. AD - Department of Cardiology, Institute of Cardiovascular Diseases, Str. Gheorghe Adam No. 13A, 300310 Timisoara, Romania. AD - Department V-Internal Medicine 1, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania. FAU - Rus, Daniel AU - Rus D AD - Doctoral School of Medicine, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Sq. No. 2, 300041 Timisoara, Romania. AD - Department of Cardiology, Institute of Cardiovascular Diseases, Str. Gheorghe Adam No. 13A, 300310 Timisoara, Romania. AD - Department V-Internal Medicine 1, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania. FAU - Caruntu, Florina Rozina AU - Caruntu FR AD - Department V-Internal Medicine 1, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania. AD - Multidisciplinary Heart Research Center, Faculty of Medicine, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania. FAU - Buz, Bogdan-Flaviu AU - Buz BF AUID- ORCID: 0000-0001-5611-2421 AD - Doctoral School of Medicine, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Sq. No. 2, 300041 Timisoara, Romania. AD - Department of Cardiology, Institute of Cardiovascular Diseases, Str. Gheorghe Adam No. 13A, 300310 Timisoara, Romania. AD - Department V-Internal Medicine 1, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania. FAU - Parvanescu, Tudor AU - Parvanescu T AD - Department V-Internal Medicine 1, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania. AD - Multidisciplinary Heart Research Center, Faculty of Medicine, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania. FAU - Andor, Minodora AU - Andor M AD - Department V-Internal Medicine 1, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania. AD - Multidisciplinary Heart Research Center, Faculty of Medicine, "Victor Babes" University of Medicine and Pharmacy of Timisoara, Eftimie Murgu Square, No. 2, 300041 Timisoara, Romania. LA - eng GR - Victor Babes University of Medicine and Pharmacy Timisoara/ PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13702357 OTO - NOTNLM OT - Lipoprotein(a) OT - Lp(a)HORIZON OT - cardiovascular outcomes OT - coronary artery disease OT - lipid-lowering therapy OT - myocardial infarction OT - residual cardiovascular risk OT - secondary prevention COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:35 PHST- 2026/09/13 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - biomedicines14102339 [pii] AID - biomedicines-14-02339 [pii] AID - 10.3390/biomedicines14102339 [doi] PST - epublish SO - Biomedicines. 2026 Oct 14;14(10):2339. doi: 10.3390/biomedicines14102339. PMID- 42940140 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 14 TI - VGLL4 Suppresses Malignant Phenotypes of Clear Cell Renal Cell Carcinoma Cells Through Modulation of Hippo/YAP and Wnt/beta-Catenin Signaling Pathways. LID - 10.3390/biomedicines14102334 [doi] LID - 2334 AB - Background/Objectives: Clear cell renal cell carcinoma (ccRCC), the most common and aggressive subtype of kidney cancer, continues to face challenges in early diagnosis and treatment, driving the need for reliable biomarkers and therapeutic targets. Vestigial-like family member 4 (VGLL4), a transcriptional cofactor, has been identified as a tumor suppressor in several malignancies. However, its clinical significance, biological functions, and underlying mechanisms in ccRCC remain largely unknown. Methods: In this study, we examined VGLL4 expression in ccRCC and normal tissues at both mRNA and protein levels and analyzed its correlation with clinicopathological features and patient prognosis. Functional experiments were performed to evaluate the effects of VGLL4 overexpression on ccRCC cell proliferation, migration, and invasion in vitro, as well as tumor growth in vivo. Integrated transcriptomic profiling and immunoblot validation were employed to explore the underlying signaling pathways. Results: We found that VGLL4 was downregulated in ccRCC tissues compared to normal counterparts at both mRNA and protein levels, and its low expression correlates with advanced disease stage and poorer overall survival. Functional experiments demonstrated that VGLL4 overexpression significantly suppressed the proliferation, migration, and invasion of ccRCC cells in vitro and effectively hindered tumor growth in vivo. Mechanistically, integrated transcriptomic profiling and immunoblot validation suggest that the tumor-suppressive effects of VGLL4 are mediated through the coordinated modulation of the Hippo/YAP and Wnt/beta-catenin signaling pathways, at least in part by attenuating the activation and transcriptional activities of YAP and beta-catenin. Conclusions: Collectively, these findings establish VGLL4 as a tumor suppressor in ccRCC and highlight its potential as a prognosis-related biomarker and candidate therapeutic target through its dual regulatory role in Hippo/YAP and Wnt/beta-catenin signaling cascades. FAU - Zhang, Yaru AU - Zhang Y AD - Department of Pharmacy, Personalized Drug Research and Therapy Key Laboratory of Sichuan Province, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610072, China. FAU - Wang, Shuyao AU - Wang S AD - Department of Pharmacy, Personalized Drug Research and Therapy Key Laboratory of Sichuan Province, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610072, China. FAU - Zhao, Zhipeng AU - Zhao Z AD - Department of Pharmacy, Evidence-Based Pharmacy Center, West China Second Hospital, Sichuan University, Chengdu 610041, China. AD - Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu 610041, China. FAU - Yuan, Tao AU - Yuan T AD - Department of Pharmacy, Personalized Drug Research and Therapy Key Laboratory of Sichuan Province, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610072, China. FAU - Zhu, Changyu AU - Zhu C AUID- ORCID: 0000-0002-7040-3898 AD - Department of Pharmacy, Personalized Drug Research and Therapy Key Laboratory of Sichuan Province, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610072, China. AD - State Key Laboratory of Neurology and Oncology Drug Development, Nanjing 210023, China. FAU - Zhong, Lei AU - Zhong L AUID- ORCID: 0000-0002-1901-9698 AD - Department of Pharmacy, Personalized Drug Research and Therapy Key Laboratory of Sichuan Province, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu 610072, China. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701626 OTO - NOTNLM OT - VGLL4 OT - YAP phosphorylation OT - ccRCC OT - dual regulation OT - prognostic indicator COIS- The State Key Laboratory of Neurology and Oncology Drug Development is hosted by the Simcere Pharmaceutical Group Limited. The authors have a research collaboration relationship with this laboratory. No other conflicts of interest to declare. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:35 PHST- 2026/08/18 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - biomedicines14102334 [pii] AID - biomedicines-14-02334 [pii] AID - 10.3390/biomedicines14102334 [doi] PST - epublish SO - Biomedicines. 2026 Oct 14;14(10):2334. doi: 10.3390/biomedicines14102334. PMID- 42940130 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 13 TI - Natural Bioactive Agents as Adjunctive Candidates in Periodontitis: Mechanistic Links to Cardiovascular Disease and Translational Challenges. LID - 10.3390/biomedicines14102324 [doi] LID - 2324 AB - Periodontitis is a highly prevalent chronic inflammatory disease that destroys the tooth-supporting tissues and has been consistently associated, in observational studies, with low-grade systemic inflammation and atherosclerotic cardiovascular disease, although whether this reflects a direct contribution to vascular disease, shared risk factors, or both remains unresolved. Subgingival instrumentation is the standard of care. Natural bioactive agents have been investigated as adjuncts because selected formulations exhibit antimicrobial, anti-inflammatory, antioxidant, pro-resolving, or reparative actions. This critical narrative review analyzes mechanistic, preclinical, and clinical evidence and asks whether effects on pathways shared by periodontal and vascular biology constitute a plausible, but unproven, link to cardiovascular health. Clinical trials have reported small, short-term changes in periodontal surrogate outcomes for selected formulations. They do not establish non-inferiority or equivalence to chlorhexidine, and none assessed cardiovascular events. Local gels, nanoparticles, and adhesive local formulations are being developed to address the poor bioavailability limiting several agents, though clinical validation remains preliminary. Formulations may improve periodontal exposure, but they add a second experimental variable and remain incompletely validated in humans. Systemically administered omega-3 fatty acids, melatonin, vitamin D, and polyphenols also require separate consideration because direct extra-periodontal effects and drug interactions can occur independently of periodontal change. Natural bioactive agents should therefore be regarded as investigational adjuncts rather than replacements for mechanical therapy. Their cardiovascular relevance remains a testable translational hypothesis that requires route-specific pharmacokinetics, human target-engagement evidence, adequately powered periodontal trials, and eventual cardiovascular outcome studies. FAU - Reytor-Gonzalez, Claudia AU - Reytor-Gonzalez C AUID- ORCID: 0009-0007-4234-5524 AD - Center for Evidence Ecosystems, Implementation Science, Decision-Making (CIDES), Facultad de Ciencias de la Salud y Bienestar Humano, Universidad Tecnologica Indoamerica, Ambato 180150, Ecuador. FAU - Campuzano-Donoso, Martin AU - Campuzano-Donoso M AUID- ORCID: 0000-0001-5317-8548 AD - Center for Evidence Ecosystems, Implementation Science, Decision-Making (CIDES), Facultad de Ciencias de la Salud y Bienestar Humano, Universidad Tecnologica Indoamerica, Ambato 180150, Ecuador. FAU - Jima Gavilanes, Dolores AU - Jima Gavilanes D AD - Escuela de Medicina, Universidad Espiritu Santo, Samborondon 0901952, Ecuador. FAU - Horowitz, Raquel AU - Horowitz R AD - Valley Health System, Geriatric Medicine, Glen Rock, NJ 07452, USA. FAU - Simancas-Racines, Daniel AU - Simancas-Racines D AD - Center for Evidence Ecosystems, Implementation Science, Decision-Making (CIDES), Facultad de Ciencias de la Salud y Bienestar Humano, Universidad Tecnologica Indoamerica, Ambato 180150, Ecuador. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13702358 OTO - NOTNLM OT - cardiovascular disease OT - drug delivery systems OT - host modulation OT - natural bioactive agents OT - periodontitis OT - polyphenols OT - systemic inflammation OT - translational medicine COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:35 PHST- 2026/08/04 00:00 [received] PHST- 2026/09/10 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - biomedicines14102324 [pii] AID - biomedicines-14-02324 [pii] AID - 10.3390/biomedicines14102324 [doi] PST - epublish SO - Biomedicines. 2026 Oct 13;14(10):2324. doi: 10.3390/biomedicines14102324. PMID- 42940124 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 12 TI - Transcriptomic Assessment of PD-L1 (CD274) in NSCLC: Biological Significance, Clinical Relevance, and Methodological Challenges. LID - 10.3390/biomedicines14102318 [doi] LID - 2318 AB - PD-L1 immunohistochemistry is widely used to guide immune checkpoint inhibitor therapy in non-small cell lung carcinoma (NSCLC), but its clinical performance is limited by spatial and temporal heterogeneity and assay-related variability. Transcriptomic assessment of CD274 may provide complementary information. We conducted a narrative review of PubMed to evaluate the biological and clinical significance of CD274 transcript expression in NSCLC, with additional consideration of PDCD1/PD-1 and methodological sources of heterogeneity. Overall, 29 studies were included, predominantly retrospective cohorts. CD274 transcript levels generally showed a positive, but variable, association with PD-L1 protein expression. Associations with histological subtype, tumour stage, smoking status, molecular alterations, and survival were inconsistent across studies. Treatment-associated findings were more promising in selected immune checkpoint inhibitor-treated cohorts. Associations were more pronounced in selected co-expression-defined subgroups, particularly for CD8A/CD274; however, the incremental value of multimarker models over single-gene assessment has not been consistently established. Differences in cellular composition, spatial heterogeneity, treatment exposure, reference-gene selection, and tissue preservation likely contributed to discordant results. In conclusion, CD274 transcript expression may complement PD-L1 immunohistochemistry in NSCLC, but current evidence is insufficient to support its use as a stand-alone prognostic or predictive biomarker. Prospective validation and methodological standardisation are required. FAU - Baron, Maciej AU - Baron M AUID- ORCID: 0000-0003-4325-5989 AD - Department of Pathomorphology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 40-055 Katowice, Poland. FAU - Jablonska, Iwona AU - Jablonska I AD - IIIrd Radiotherapy and Chemotherapy Department, Maria Sklodowska-Curie National Research Institute of Oncology, 44-102 Gliwice, Poland. FAU - Lewandowski, Piotr AU - Lewandowski P AD - Department of Histology and Cell Pathology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 40-055 Katowice, Poland. FAU - Wachowicz, Malgorzata AU - Wachowicz M AD - Department of Histology and Cell Pathology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 40-055 Katowice, Poland. FAU - Tomkiewicz, Eliza AU - Tomkiewicz E AD - Department of Histology and Cell Pathology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 40-055 Katowice, Poland. FAU - Skrzypiec, Adam AU - Skrzypiec A AUID- ORCID: 0009-0001-0496-2720 AD - Department of Histology and Cell Pathology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 40-055 Katowice, Poland. FAU - Drozdzowska, Bogna AU - Drozdzowska B AD - Department of Pathomorphology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 40-055 Katowice, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701400 OTO - NOTNLM OT - CD274 OT - PD-1 OT - PD-L1 OT - PDCD1 OT - RNA expression OT - biomarkers OT - immunohistochemistry OT - non-small cell lung carcinoma (NSCLC) OT - prognosis OT - transcriptomics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:35 PHST- 2026/08/16 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - biomedicines14102318 [pii] AID - biomedicines-14-02318 [pii] AID - 10.3390/biomedicines14102318 [doi] PST - epublish SO - Biomedicines. 2026 Oct 12;14(10):2318. doi: 10.3390/biomedicines14102318. PMID- 42940122 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 12 TI - From Proteostasis Disruption to Stone Formation: Ubiquitin-Dependent Regulatory Networks in Calcium Oxalate Nephrolithiasis. LID - 10.3390/biomedicines14102316 [doi] LID - 2316 AB - Calcium oxalate (CaOx) nephrolithiasis is a multistep disease process in which urinary supersaturation and crystallization interact with renal tissue responses to promote persistent mineral deposition and stone development. Two related pathological settings are particularly relevant: tubular crystal-epithelial interactions, where crystal-induced cellular stress, inflammation, regulated cell death, and epithelial surface remodeling favor crystal adhesion and retention; and Randall's plaque (RP)-associated papillary mineralization, where interstitial mineral deposition and plaque exposure provide a substrate for CaOx overgrowth and stone initiation. Although pathologically distinct, both involve changes in protein homeostasis and cellular phenotype. The ubiquitin-proteasome system (UPS) and deubiquitinating enzymes (DUBs), through reversible control of protein stability, localization, and signaling, may regulate multiple steps in these processes. This review examines ubiquitin-dependent regulation across tubular injury and crystal retention, inflammatory signaling, cellular stress and organelle homeostasis, regulated cell death, and RP-associated remodeling. Disease-specific studies have identified mechanistically resolved pathways, including the VHL-BICD2-STAT1, Lgals3-TRIM21-PKM2, and PRMT1-UBE2M-NEDD4-PPARgamma axes in CaOx-associated tubular pathology, as well as the OMD-NEDD4-BMP2 and TRAF1-TRAF2/CBLC axes in RP-associated pathology. Other findings implicate ubiquitin-dependent regulation in mitochondrial quality control, inflammation, epithelial remodeling, and cell death, but the evidence remains uneven. Only a limited number of E3 ligase/DUB-substrate relationships have been established directly in CaOx or RP models, while many candidate mechanisms derive from other renal or non-renal disease contexts. Viewed through the lens of proteostasis, altered protein fate may connect cellular stress with epithelial dysfunction, crystal retention, and papillary mineralization without implying a single route to stone formation. Defining CaOx-specific ubiquitin networks will require quantitative ubiquitinomics and ubiquitin-chain analysis integrated with spatial approaches and causal validation in disease-relevant models and human tissues. Such studies may reveal therapeutically tractable targets with sufficient molecular and renal selectivity. FAU - Wu, Guanwei AU - Wu G AUID- ORCID: 0009-0007-3793-1190 AD - Department of Urology, Affiliated Wuhu Hospital of East China Normal University, Wuhu 241001, China. FAU - Liu, Hualin AU - Liu H AD - Graduate School, Wannan Medical University, Wuhu 241001, China. FAU - Zhu, Weiyu AU - Zhu W AD - Department of Ultrasound, Affiliated Wuhu Hospital of East China Normal University, Wuhu 241001, China. FAU - Liu, Yingqing AU - Liu Y AD - Department of Urology, Affiliated Wuhu Hospital of East China Normal University, Wuhu 241001, China. FAU - Qin, Haibo AU - Qin H AD - Department of Urology, Affiliated Wuhu Hospital of East China Normal University, Wuhu 241001, China. FAU - Tao, Lingsong AU - Tao L AD - Department of Urology, Affiliated Wuhu Hospital of East China Normal University, Wuhu 241001, China. LA - eng GR - AHWJ2025Ab40104/Anhui Provincial Health Science and Technology Project/ GR - 2023jc32/Key Project of Wuhu Science and Technology Bureau/ GR - 2024A04/Research Project of the Affiliated Wuhu Hospital of East China Normal University/ PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701867 OTO - NOTNLM OT - calcium oxalate nephrolithiasis OT - crystal adhesion OT - deubiquitinating enzymes OT - proteostasis OT - regulated cell death OT - ubiquitin-proteasome system COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:35 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - biomedicines14102316 [pii] AID - biomedicines-14-02316 [pii] AID - 10.3390/biomedicines14102316 [doi] PST - epublish SO - Biomedicines. 2026 Oct 12;14(10):2316. doi: 10.3390/biomedicines14102316. PMID- 42940114 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 11 TI - Echocardiographic Left Ventricular Hypertrophy Among Patients with Type 2 Diabetes Undergoing Clinically Indicated Echocardiography at a Saudi Cardiac Center: Prevalence and Associated Factors. LID - 10.3390/biomedicines14102308 [doi] LID - 2308 AB - Background/Objectives: Left ventricular hypertrophy (LVH) is an important cardiovascular complication, but regional data on this condition among patients with type 2 diabetes mellitus (T2DM) in Saudi Arabia remain limited. This study determined the prevalence of LVH and associated factors among adults with T2DM without documented cardiac disease undergoing clinically indicated echocardiography at a specialized cardiac center in Saudi Arabia. Methods: This retrospective cross-sectional study included 625 patients with T2DM who underwent clinically indicated echocardiography at Prince Sultan Cardiac Center, Qassim, between January 2023 and December 2025. Of 878 patients with T2DM and no documented cardiac disease assessed for eligibility, 253 (28.8%) were excluded because at least one required clinical variable was missing or unavailable, leaving 625 patients (71.2%) in the final analysis. LVH was assessed according to American Society of Echocardiography/European Association of Cardiovascular Imaging recommendations. Multivariable logistic regression was used to evaluate factors associated with LVH. Results: LVH was identified in 146/625 patients (23.4%; 95% CI, 20.0-26.7). After multivariable adjustment, male sex (aOR = 1.71, 95% CI: 1.11-2.63; p = 0.015) and uncontrolled/elevated blood pressure (aOR = 2.18, 95% CI: 1.26-3.80; p = 0.006) were associated with LVH. Diabetes duration showed a significant nonlinear association with LVH (overall p < 0.001; p for nonlinearity = 0.001). Conclusions: LVH was common among this clinically selected group of patients with T2DM undergoing clinically indicated echocardiography. Male sex and uncontrolled/elevated blood pressure were associated with LVH after adjustment, while diabetes duration showed a nonlinear association. Although causal relationships cannot be established, the observed association between uncontrolled/elevated blood pressure and LVH highlights the importance of optimal blood pressure management as part of comprehensive cardiovascular risk reduction in patients with T2DM. FAU - Alsaud, Jolan AU - Alsaud J AUID- ORCID: 0000-0003-0067-7479 AD - Family Medicine, Qassim Health Cluster, Buraydah 52387, Saudi Arabia. FAU - Aldkheel, Mohammed AU - Aldkheel M AD - Prince Sultan Cardiac Center, Qassim Health Cluster, Buraydah 52387, Saudi Arabia. FAU - Alenazi, Fatimah AU - Alenazi F AUID- ORCID: 0000-0001-5701-9106 AD - Department of Public Health, College of Applied Medical Sciences, Qassim University, Buraydah 52387, Saudi Arabia. FAU - Alharbi, Razan AU - Alharbi R AUID- ORCID: 0009-0008-5310-2437 AD - Department of Family and Community Medicine, College of Medicine, Qassim University, Buraydah 52387, Saudi Arabia. FAU - Alsuhaibani, Reef AU - Alsuhaibani R AD - Family Medicine, Qassim Health Cluster, Buraydah 52387, Saudi Arabia. FAU - Almotairi, Joud AU - Almotairi J AD - Family Medicine, Qassim Health Cluster, Buraydah 52387, Saudi Arabia. FAU - Alharbi, Sali AU - Alharbi S AD - Department of Family and Community Medicine, College of Medicine, Qassim University, Buraydah 52387, Saudi Arabia. FAU - Alharbi, Lyan AU - Alharbi L AUID- ORCID: 0009-0001-0305-7729 AD - Department of Family and Community Medicine, College of Medicine, Qassim University, Buraydah 52387, Saudi Arabia. FAU - Alshalahi, Emtenan AU - Alshalahi E AUID- ORCID: 0009-0006-1303-245X AD - Department of Family and Community Medicine, College of Medicine, Qassim University, Buraydah 52387, Saudi Arabia. FAU - Alamro, Alwaleed AU - Alamro A AD - Department of Family and Community Medicine, College of Medicine, Qassim University, Buraydah 52387, Saudi Arabia. FAU - Algeffari, Metab AU - Algeffari M AUID- ORCID: 0000-0002-0614-2558 AD - Department of Family and Community Medicine, College of Medicine, Qassim University, Buraydah 52387, Saudi Arabia. AD - Abdullah Al Othaim Diabetes Center, Qassim University Medical City, Qassim University, Buraydah 52387, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261011 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13702396 OTO - NOTNLM OT - Saudi Arabia OT - blood pressure OT - cross-sectional studies OT - echocardiography OT - hypertension OT - left ventricular hypertrophy OT - left ventricular mass index OT - type 2 diabetes mellitus COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/11 CRDT- 2026/10/27 10:35 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - biomedicines14102308 [pii] AID - biomedicines-14-02308 [pii] AID - 10.3390/biomedicines14102308 [doi] PST - epublish SO - Biomedicines. 2026 Oct 11;14(10):2308. doi: 10.3390/biomedicines14102308. PMID- 42940106 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 10 TI - Short-Term Clinical Outcomes of Ready-Made Soft Splints Versus Digitally Fabricated, Customized Hard Stabilization Splints in Temporomandibular Disorders: An Exploratory Randomized Study. LID - 10.3390/biomedicines14102299 [doi] LID - 2299 AB - Background/Objectives: Occlusal splints are widely used for temporomandibular disorders (TMDs), but direct evidence comparing ready-made soft splints with customized hard stabilization splints remains limited. This exploratory randomized study compared the short-term clinical outcomes of a ready-made soft splint and a digitally fabricated, customized 3D-printed hard stabilization splint, representing two distinct fabrication and delivery workflows. Methods: Seventy adults with TMDs were randomized 1:1 to a customized hard splint fabricated using intraoral scanning and digital light processing 3D printing or to a ready-made soft splint (n = 35 per group); 68 completed follow-up. Modified Helkimo index scores were assessed at baseline (T0), 4 weeks (T1), and 12 weeks (T2). Results: Scores decreased significantly from baseline in both groups at T1 and T2 (all p < 0.001). No statistically significant between-group difference was detected in change from baseline to T1 (Hodges-Lehmann median difference, hard minus soft = 0.00; 95% CI, -1.00 to 1.00; p = 0.573) or T2 (-1.00; 95% CI, -2.00 to 0.00; p = 0.209). Conclusions: No statistically significant difference in short-term clinical improvement was detected between the two splint groups. This finding should not be interpreted as evidence of equivalence or non-inferiority. Ready-made soft splints may have a limited, hypothesis-generating role as a short-term conservative or bridging option for selected patients, but adequately powered, prospectively registered non-inferiority trials are needed to define their clinical role. Trial Registration: CRIS KCT0012318 (retrospectively registered). FAU - Park, Sang-Yoon AU - Park SY AUID- ORCID: 0000-0001-7624-3373 AD - Department of Oral and Maxillofacial Surgery, Hallym University Sacred Heart Hospital, Anyang 14066, Republic of Korea. AD - Department of Artificial Intelligence and Robotics in Dentistry, Graduate School of Clinical Dentistry, Hallym University, Chuncheon 24252, Republic of Korea. AD - Institute of Clinical Dentistry, Hallym University, Chuncheon 24252, Republic of Korea. AD - Dental Artificial Intelligence and Robotics R&D Center, Hallym University Medical Center, Anyang 14066, Republic of Korea. FAU - Byun, Soo-Hwan AU - Byun SH AD - Department of Oral and Maxillofacial Surgery, Hallym University Sacred Heart Hospital, Anyang 14066, Republic of Korea. AD - Department of Artificial Intelligence and Robotics in Dentistry, Graduate School of Clinical Dentistry, Hallym University, Chuncheon 24252, Republic of Korea. AD - Institute of Clinical Dentistry, Hallym University, Chuncheon 24252, Republic of Korea. AD - Dental Artificial Intelligence and Robotics R&D Center, Hallym University Medical Center, Anyang 14066, Republic of Korea. FAU - Baek, Sae-Hoon AU - Baek SH AD - Department of Oral and Maxillofacial Surgery, Hallym University Sacred Heart Hospital, Anyang 14066, Republic of Korea. AD - Department of Artificial Intelligence and Robotics in Dentistry, Graduate School of Clinical Dentistry, Hallym University, Chuncheon 24252, Republic of Korea. AD - Institute of Clinical Dentistry, Hallym University, Chuncheon 24252, Republic of Korea. AD - Dental Artificial Intelligence and Robotics R&D Center, Hallym University Medical Center, Anyang 14066, Republic of Korea. FAU - Che, Sung-Ah AU - Che SA AUID- ORCID: 0009-0001-8587-5134 AD - Department of Oral and Maxillofacial Surgery, Hallym University Sacred Heart Hospital, Anyang 14066, Republic of Korea. AD - Department of Artificial Intelligence and Robotics in Dentistry, Graduate School of Clinical Dentistry, Hallym University, Chuncheon 24252, Republic of Korea. AD - Institute of Clinical Dentistry, Hallym University, Chuncheon 24252, Republic of Korea. AD - Dental Artificial Intelligence and Robotics R&D Center, Hallym University Medical Center, Anyang 14066, Republic of Korea. FAU - Cho, Seoung-Won AU - Cho SW AUID- ORCID: 0000-0002-3955-5623 AD - Department of Artificial Intelligence and Robotics in Dentistry, Graduate School of Clinical Dentistry, Hallym University, Chuncheon 24252, Republic of Korea. AD - Institute of Clinical Dentistry, Hallym University, Chuncheon 24252, Republic of Korea. AD - Dental Artificial Intelligence and Robotics R&D Center, Hallym University Medical Center, Anyang 14066, Republic of Korea. FAU - Park, In-Young AU - Park IY AD - Department of Artificial Intelligence and Robotics in Dentistry, Graduate School of Clinical Dentistry, Hallym University, Chuncheon 24252, Republic of Korea. AD - Institute of Clinical Dentistry, Hallym University, Chuncheon 24252, Republic of Korea. AD - Dental Artificial Intelligence and Robotics R&D Center, Hallym University Medical Center, Anyang 14066, Republic of Korea. AD - Department of Orthodontics, Hallym University Sacred Heart Hospital, Anyang 14066, Republic of Korea. FAU - Yang, Byoung-Eun AU - Yang BE AUID- ORCID: 0000-0002-4446-6772 AD - Department of Oral and Maxillofacial Surgery, Hallym University Sacred Heart Hospital, Anyang 14066, Republic of Korea. AD - Department of Artificial Intelligence and Robotics in Dentistry, Graduate School of Clinical Dentistry, Hallym University, Chuncheon 24252, Republic of Korea. AD - Institute of Clinical Dentistry, Hallym University, Chuncheon 24252, Republic of Korea. AD - Dental Artificial Intelligence and Robotics R&D Center, Hallym University Medical Center, Anyang 14066, Republic of Korea. LA - eng PT - Journal Article DEP - 20261010 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13702428 OTO - NOTNLM OT - 3D printing OT - digital workflow OT - exploratory randomized study OT - modified Helkimo index OT - occlusal splint OT - soft splint OT - stabilization splint OT - temporomandibular disorders COIS- No author has a personal financial relationship with Balancetech Corp. (Seongnam, Republic of Korea), the manufacturer of the ready-made soft splint used in this study. Balance dental hospital served as one of the two clinical recruitment sites and has a commercial association with Balancetech Corp., which is a legally independent entity. The authors acknowledge this as a potential site-level conflict of interest. To minimize related bias, identical recruitment criteria, independent randomization, standardized outcome assessment protocols, and a baseline- and institution-adjusted sensitivity analysis (Supplementary Table S1) were applied. All other aspects of the study were conducted independently of device manufacturing and supply. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:35 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - biomedicines14102299 [pii] AID - biomedicines-14-02299 [pii] AID - 10.3390/biomedicines14102299 [doi] PST - epublish SO - Biomedicines. 2026 Oct 10;14(10):2299. doi: 10.3390/biomedicines14102299. PMID- 42940102 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 9 TI - Reference Genome Choice Shapes RNA-Seq Quantification and Downstream Interpretation in Chinese Breast Cancer Patients. LID - 10.3390/biomedicines14102296 [doi] LID - 2296 AB - Background/Objectives: Reference genome choice can introduce systematic bias into cancer transcriptomic analyses, particularly for populations underrepresented in standard references. However, its impact on downstream biological interpretation remains insufficiently characterized. Methods: We compared GRCh38, T2T-CHM13, and the East Asian-matched T2T-YAO using bulk RNA-seq data from 148 Chinese breast cancer patients and single-cell RNA-seq data comprising 65,968 cells from paired primary tumors and lymph node metastases. We assessed reference-dependent differences in read mapping, gene expression, and downstream transcriptomic analyses. Results: Compared with GRCh38, YAO reduced multi-mapped reads approximately three-fold and increased confidently exon-assigned reads. Re-mapping YAO-assigned reads to GRCh38 showed that 19.91% changed gene assignment, with a mean of 1016 genes per sample exhibiting more than ten-fold expression differences. Across the cohort, 8432 genes showed such discrepancies, and 68.49% overlapped low-mappability regions in YAO. These genes were enriched in receptor, ion channel, and drug metabolism functions. Reference-dependent expression differences propagated to differential expression, immune cell inference, fusion detection, and classifier performance. In single-cell analyses, T2T references retained more cells after quality filtering and resolved a fibroblast subpopulation missed by GRCh38. Conclusions: Reference genome selection substantially influences RNA-seq quantification and downstream biological interpretation in breast cancer. Population-matched and complete T2T reference genomes can reduce mapping ambiguity and improve the resolution of transcriptomic features, highlighting reference genome choice as an important and underappreciated source of systematic bias in cancer transcriptomics. FAU - Zhang, Ruiyang AU - Zhang R AD - China National Center for Bioinformation, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China. AD - Beijing Institute of Genomics, University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Peng, Yuan AU - Peng Y AD - Breast Center, Peking University People's Hospital, Beijing 100044, China. FAU - Zhang, Mengmeng AU - Zhang M AD - Breast Center, Peking University People's Hospital, Beijing 100044, China. FAU - Chu, Yanan AU - Chu Y AUID- ORCID: 0000-0002-9349-4307 AD - China National Center for Bioinformation, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China. FAU - Shao, Changjun AU - Shao C AD - China National Center for Bioinformation, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China. FAU - Huang, Zhuo AU - Huang Z AUID- ORCID: 0009-0005-1023-1560 AD - China National Center for Bioinformation, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China. AD - Beijing Institute of Genomics, University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Yang, Yiji AU - Yang Y AD - China National Center for Bioinformation, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China. AD - Beijing Institute of Genomics, University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Chen, Jing AU - Chen J AD - China National Center for Bioinformation, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China. FAU - Kang, Yu AU - Kang Y AUID- ORCID: 0000-0001-5196-0376 AD - China National Center for Bioinformation, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, China. AD - Beijing Institute of Genomics, University of Chinese Academy of Sciences, Beijing 100049, China. FAU - Wang, Shu AU - Wang S AUID- ORCID: 0000-0002-8651-4795 AD - Breast Center, Peking University People's Hospital, Beijing 100044, China. LA - eng GR - 2024YFC3405704/National Key Research and Development Program of China/ GR - 32371537/National Science Foundation of China/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701484 OTO - NOTNLM OT - RNA-seq OT - T2T-YAO OT - biomarker discovery OT - breast cancer OT - population-matched reference genome OT - single-cell RNA-seq OT - telomere-to-telomere genome OT - transcriptomic inference COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:35 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - biomedicines14102296 [pii] AID - biomedicines-14-02296 [pii] AID - 10.3390/biomedicines14102296 [doi] PST - epublish SO - Biomedicines. 2026 Oct 9;14(10):2296. doi: 10.3390/biomedicines14102296. PMID- 42940101 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 9 TI - TPD54 Downregulates ERK Signaling in Oral Squamous Cell Carcinoma Cells. LID - 10.3390/biomedicines14102295 [doi] LID - 2295 AB - Background/Objectives: Epidermal growth factor receptor is frequently overexpressed in oral squamous cell carcinoma (OSCC) and is associated with poor prognosis. We previously demonstrated that members of the tumor protein D (TPD) 52 family regulate the malignant behavior of OSCC cells and that epidermal growth factor (EGF) induces TPD52 expression. In this study, we investigated the relationship between the TPD52 family and EGF signaling, focusing on TPD54. Methods: SAS OSCC cells were treated with EGF and gefitinib, and the expression of TPD52 family members (TPD52, TPD53, and TPD54) was analyzed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blotting. Nascent RNA and RNA degradation assays were conducted to investigate the mechanisms regulating TPD52 family expression. TPD54-overexpressing and TPD54-knockdown cells were used to evaluate EGF signaling, cell cycle progression, proliferation, apoptosis, autophagy, migration, and invasion. Results: EGF stimulation increased the mRNA expression of all TPD52 family members. Nascent RNA analysis revealed increased transcription, whereas RNA degradation assays indicated reduced mRNA stability. TPD54 overexpression attenuated EGF-induced ERK phosphorylation and suppressed EGF-induced cell cycle progression. However, under the present experimental conditions, TPD54 had little effect on cell proliferation, apoptosis, autophagy, migration, or invasion. Conclusions: TPD54 is an EGF-responsive protein that suppresses EGF-induced ERK activation and attenuates cell cycle progression in OSCC cells. Although TPD54 exerted minimal effects on cell proliferation, apoptosis, autophagy, invasion, or migration under the tested conditions, these findings suggest that TPD54 functions as a negative regulator of EGF signaling and provide insight into its molecular role in OSCC. FAU - Yamada, Konomi AU - Yamada K AD - Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ota-ku 145-8515, Tokyo, Japan. FAU - Mukudai, Yoshiki AU - Mukudai Y AUID- ORCID: 0000-0002-9600-4430 AD - Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ota-ku 145-8515, Tokyo, Japan. FAU - Nara, Maki AU - Nara M AD - Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ota-ku 145-8515, Tokyo, Japan. FAU - Watanabe, Masataka AU - Watanabe M AD - Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ota-ku 145-8515, Tokyo, Japan. FAU - Kindaichi, Nodoka AU - Kindaichi N AD - Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ota-ku 145-8515, Tokyo, Japan. FAU - Shimane, Toshikazu AU - Shimane T AUID- ORCID: 0000-0002-0816-0223 AD - Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ota-ku 145-8515, Tokyo, Japan. FAU - Shirota, Tatsuo AU - Shirota T AD - Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ota-ku 145-8515, Tokyo, Japan. FAU - Ohba, Seigo AU - Ohba S AUID- ORCID: 0000-0002-0639-1714 AD - Department of Oral and Maxillofacial Surgery, School of Dentistry, Showa University, 2-1-1 Kitasenzoku, Ota-ku 145-8515, Tokyo, Japan. LA - eng GR - 23K16166/Japan Society for the Promotion of Science/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701923 OTO - NOTNLM OT - oral cancer OT - squamous cell carcinoma OT - tumor protein D52 family OT - tumor protein D54 COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:35 PHST- 2026/08/18 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - biomedicines14102295 [pii] AID - biomedicines-14-02295 [pii] AID - 10.3390/biomedicines14102295 [doi] PST - epublish SO - Biomedicines. 2026 Oct 9;14(10):2295. doi: 10.3390/biomedicines14102295. PMID- 42940100 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 9 TI - Endogenous APP-Associated Proteomics in Mouse Brain Defines a Candidate Resource for APP Trafficking and Proteostasis Studies. LID - 10.3390/biomedicines14102293 [doi] LID - 2293 AB - Background/Objectives: Affinity proteomics of amyloid precursor protein (APP) is complicated by proteolytic processing, dynamic trafficking, and purification background. We generated a C-terminal 3xFLAG knock-in at the endogenous App locus to establish a cortical affinity-proteomic resource and prioritize APP-associated candidates for further study. Methods: Eight-week-old male homozygous APP-3xFLAG mice on a C57BL/6J background and wild-type littermates were characterized by biochemical and behavioral assays (n = 6 and n = 7 per genotype, respectively). Cortical material from three APP-3xFLAG males was pooled for anti-FLAG affinity purification and liquid chromatography-tandem mass spectrometry. Protein accessions were filtered by positive Area, unique-peptide support, and control recovery, with Gene Ontology (GO) analyses conducted under alternative backgrounds. Selected candidates underwent targeted co-immunoprecipitation and immunoblotting. Results: Baseline APP, amyloid-beta, and behavioral measurements showed no statistically significant genotype-associated differences. Five shared high-confidence APP regions showed local Calpha RMSD values below 0.3 A in structural-model comparisons. Across APP-IP and control datasets, 1874 accessions were identified. Evidence filtering retained 990 non-bait candidate accessions representing 967 APP-IP protein groups. Two de novo-only spectra supported recovery of the engineered APP-3xFLAG junction peptide. GO analysis identified 21 significant cellular-component terms under the observed-union background, with none under the evidence-eligible background. Targeted anti-FLAG co-immunoprecipitation and immunoblotting supported ANXA2 and RACK1 co-recovery with APP-3xFLAG. Conclusions: The endogenous tagging model, evidence-filtered proteomic dataset, and targeted biochemical findings establish a candidate resource for investigating APP-associated assemblies and prioritizing proteins for studies of trafficking and proteostasis. FAU - Zeng, Yeting AU - Zeng Y AD - Department of Neurology, Fujian Provincial Hospital, Fuzhou University Affiliated Provincial Hospitall, Fuzhou 350001, China. AD - Department of Neurology, Shengli Clinical Medical College of Fujian Medical University, Fuzhou 350001, China. FAU - Wei, Shushan AU - Wei S AD - Department of Neurosurgery, Fujian Provincial Hospital, Fuzhou University Affiliated Provincial Hospital, Fuzhou 350001, China. FAU - Hu, Keyi AU - Hu K AD - State Key Laboratory of Brain Function and Disorders, Institutes of Brain Science, Fudan University, Shanghai 200433, China. FAU - Zhao, Ruoqi AU - Zhao R AD - State Key Laboratory of Brain Function and Disorders, Institutes of Brain Science, Fudan University, Shanghai 200433, China. AD - Department of Neurology, Zhongshan Hospital Affiliated to Fudan University, Shanghai 200433, China. FAU - Yu, Shiwen AU - Yu S AD - State Key Laboratory of Brain Function and Disorders, Institutes of Brain Science, Fudan University, Shanghai 200433, China. AD - Department of Neurology, Zhongshan Hospital Affiliated to Fudan University, Shanghai 200433, China. FAU - Yu, Tong AU - Yu T AD - State Key Laboratory of Brain Function and Disorders, Institutes of Brain Science, Fudan University, Shanghai 200433, China. AD - Department of Neurology, Zhongshan Hospital Affiliated to Fudan University, Shanghai 200433, China. FAU - Huang, Biqing AU - Huang B AD - Department of Nuclear Medicine, Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou University Affiliated Provincial Hospitall, Fuzhou 350001, China. AD - Fujian Research Institute of Nuclear Medicine, Fuzhou 350001, China. FAU - Duan, Lijie AU - Duan L AD - Department of Neurology, Jinshan Hospital Affiliated to Fudan University, Shanghai 201500, China. FAU - Zhao, Xiangteng AU - Zhao X AUID- ORCID: 0009-0008-8760-104X AD - State Key Laboratory of Brain Function and Disorders, Institutes of Brain Science, Fudan University, Shanghai 200433, China. AD - Department of Neurology, Zhongshan Hospital Affiliated to Fudan University, Shanghai 200433, China. LA - eng GR - 32100826/National Natural Science Foundation of China/ GR - 2022M710775/China Postdoctoral Science Foundation/ GR - JSKJ-KTQN-2022-05/the Health Commission of Jinshan District, Shanghai/ GR - 2025J01502/Fujian Provincial Natural Science Foundation/ GR - 2026J001234/Fujian Provincial Natural Science Foundation/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701415 OTO - NOTNLM OT - Gene Ontology OT - affinity proteomics OT - amyloid precursor protein OT - endogenous tagging OT - mouse cortex OT - protein candidates COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:35 PHST- 2026/08/24 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - biomedicines14102293 [pii] AID - biomedicines-14-02293 [pii] AID - 10.3390/biomedicines14102293 [doi] PST - epublish SO - Biomedicines. 2026 Oct 9;14(10):2293. doi: 10.3390/biomedicines14102293. PMID- 42940099 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 9 TI - Pan-Cancer Analysis Identifies LUC7L2 as a Prognostic Biomarker and Potential Functional Regulator of Prostate Cancer Progression. LID - 10.3390/biomedicines14102294 [doi] LID - 2294 AB - Background/Objectives: Cancer imposes a substantial global public health burden, and its initiation and progression involve complex dysregulation of gene expression regulatory networks. Methods: This study systematically evaluated the expression characteristics and prognostic value of LUC7L2 across pan-cancer types, with a particular focus on functional validation in prostate cancer. Using transcriptomic and proteomic data from multiple databases, immunohistochemical staining from the Human Protein Atlas, and single-cell transcriptomic datasets GSE137829 and GSE176031, we analyzed the expression patterns of LUC7L2 in various cancer types. Diagnostic and prognostic potential was assessed through ROC curve analysis, Cox regression, single-cell, and spatial transcriptomics, while the functional role of LUC7L2 in prostate cancer tumorigenesis and progression was validated via in vitro assays. Results: Results showed that LUC7L2 was significantly upregulated in glioblastoma and prostate adenocarcinoma, and downregulated in lung adenocarcinoma and ovarian serous cystadenocarcinoma, with diagnostic AUC values exceeding 0.7 across multiple tumor types. In prostate cancer, high LUC7L2 expression was significantly associated with a shortened progression-free interval (multivariate HR = 3.50, 95% CI 1.95-6.29, p < 0.001), with a meta-analysis of independent cohorts showing a consistent direction of effect (pooled HR = 1.14, 95% CI 0.90-1.44). Moreover, LUC7L2 exhibited strong correlations with microsatellite instability and tumor mutational burden across multiple cancer types. Single-cell analysis revealed that its expression was markedly higher in malignant cells than in immune or stromal cells. In vitro knockdown of LUC7L2 in prostate cancer cells led to significantly suppressed proliferation, increased apoptosis, S-phase cell cycle arrest, reduced invasion and migration capacities, enhanced senescence, and aggravated DNA damage. Conclusions: This study demonstrates that LUC7L2 is specifically overexpressed in malignant prostate cancer cells and independently associated with poor prognosis. It further validates its regulatory roles in proliferation, apoptosis, migration, and DNA damage repair, providing both theoretical and experimental evidence supporting LUC7L2 as a potential biomarker for prostate cancer. FAU - Yuan, Jialin AU - Yuan J AD - Department of Oncology, Dongguan Hospital of Guangzhou University of Chinese Medicine, Dongguan 523000, China. AD - Department of Radiation Oncology, Dongguan Hospital of Guangzhou University of Chinese Medicine, Dongguan 523000, China. FAU - Zhan, Ping AU - Zhan P AD - Department of Surgery, Dongguan Hospital of Guangzhou University of Chinese Medicine, Dongguan 523000, China. FAU - Liu, Jun AU - Liu J AD - Department of Laboratory Medicine, Dongguan Hospital of Guangzhou University of Chinese Medicine, Dongguan 523000, China. FAU - Lu, Guangming AU - Lu G AD - Department of Surgery, Dongguan Hospital of Guangzhou University of Chinese Medicine, Dongguan 523000, China. FAU - Wang, Han AU - Wang H AD - Department of Oncology, Dongguan Hospital of Guangzhou University of Chinese Medicine, Dongguan 523000, China. FAU - Xiao, Meiqin AU - Xiao M AD - Department of Radiation Oncology, Dongguan Hospital of Guangzhou University of Chinese Medicine, Dongguan 523000, China. FAU - Li, Dandan AU - Li D AD - Department of Oncology, Dongguan Hospital of Guangzhou University of Chinese Medicine, Dongguan 523000, China. AD - Department of Radiation Oncology, Dongguan Hospital of Guangzhou University of Chinese Medicine, Dongguan 523000, China. LA - eng GR - 20261433/Traditional Chinese Medicine Bureau of Guangdong Province/ GR - 32202506002/High-level Talent Training Project of Dongguan Hospital of Traditional Chinese Medicin/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13702000 OTO - NOTNLM OT - DNA damage OT - LUC7L2 OT - biomarker OT - multi-omics OT - prostate cancer COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:35 PHST- 2026/08/22 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - biomedicines14102294 [pii] AID - biomedicines-14-02294 [pii] AID - 10.3390/biomedicines14102294 [doi] PST - epublish SO - Biomedicines. 2026 Oct 9;14(10):2294. doi: 10.3390/biomedicines14102294. PMID- 42940098 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 9 TI - The Dark Side of Radiotherapy: Experimental Evidence, Biological Mechanisms, and Preventive Strategies for Radiation-Induced Tumor-Promoting Niches. LID - 10.3390/biomedicines14102292 [doi] LID - 2292 AB - Radiotherapy (RT) is an essential treatment modality for solid tumors and achieves tumor control primarily through radiation-induced cytotoxicity. However, its biological effects extend beyond direct tumor-cell killing. Radiation inevitably affects surrounding normal and tumor-adjacent tissues, inducing inflammatory, stromal, extracellular-matrix, vascular, and cellular remodeling that can persist after treatment. Accumulating experimental evidence indicates that these tissue responses can generate microenvironments permissive to the survival and regrowth of residual tumor cells, local reseeding, and metastatic colonization. This review examines radiation-induced tumor-promoting niches as an unintended consequence of tissue injury and repair. Experimental and clinical evidence supporting their existence is summarized, followed by discussion of the major biological processes underlying their formation, including immune remodeling, fibroblast activation and extracellular-matrix reorganization, vascular injury and hypoxia-driven reparative vasculogenesis, and cellular senescence. Emerging strategies to prevent or attenuate these niches are also discussed, including limiting normal-tissue radiation exposure and selectively targeting persistent inflammatory and stromal responses, post-radiation vasculogenesis, and senescent cells. Although direct causal evidence remains predominantly preclinical and the clinical significance of these responses remains to be established, understanding how irradiated tissues evolve after treatment may reveal opportunities to preserve the therapeutic benefits of RT while minimizing long-term tissue conditions that favor tumor recurrence or metastatic progression. FAU - Choi, Yong June AU - Choi YJ AUID- ORCID: 0009-0009-8661-4815 AD - Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20261009 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701221 OTO - NOTNLM OT - radiation-induced tumor-promoting niches OT - radiotherapy COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:35 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - biomedicines14102292 [pii] AID - biomedicines-14-02292 [pii] AID - 10.3390/biomedicines14102292 [doi] PST - epublish SO - Biomedicines. 2026 Oct 9;14(10):2292. doi: 10.3390/biomedicines14102292. PMID- 42940089 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 8 TI - Lung Ultrasound in Rheumatoid Arthritis-Associated Interstitial Lung Disease: Association with Radiological Patterns, Lung Function Tests, and Disease Activity-A Single-Center Retrospective Cross-Sectional Study. LID - 10.3390/biomedicines14102281 [doi] LID - 2281 AB - Introduction: Interstitial lung disease (ILD) represents one of the most relevant extra-articular manifestations in rheumatoid arthritis (RA), and significantly contributes to morbidity and mortality. High-resolution computed tomography (HRCT) is the gold standard for the diagnosis of ILD; however, its use in daily practice is limited. Lung ultrasound (LUS) is a non-invasive, repeatable, and bedside tool for ILD assessment. This study aimed to evaluate the association between quantitative LUS score and HRCT findings in RA patients. Methods: We conducted a single-center, retrospective cross-sectional study in RA patients with and without ILD. Demographic, clinical, lung function, and disease activity (DAS28-CRP) data were collected, together with LUS and HRCT findings at baseline. Correlation and regression analyses were performed to evaluate associations between LUS score, HRCT patterns, pulmonary function, and disease activity. Results: Thirty-seven RA subjects (M/F: 20/17; mean age 68.8 +/- 10.4 years) were included; ILD was present in 24 patients (64.8%), including 17 (45.9%) with a UIP pattern. LUS score inversely correlated with DLCO (r = -0.413; p = 0.01) and positively with age (r = 0.47; p = 0.003) and DAS28-CRP (r = 0.366; p = 0.02). Higher LUS score were significantly associated with UIP pattern at both uni- (OR 1.09; 95%CI 1.02-1.16; p < 0.01) and multivariable regression analysis adjusted for age (OR 1.07; 95%CI 1.01-1.15; p = 0.02). Likewise, DAS28-CRP was significantly associated with a UIP pattern on chest HRCT in both univariable (OR 3.05, 95% CI 1.30-7.14; p = 0.01) and multivariable analyses adjusted for age (OR 2.35, 95% CI 1.19-6.86; p = 0.02). Conclusions: Overall, higher LUS score values were associated with reduced pulmonary function, higher DAS28-CRP, and the presence of a UIP pattern on chest HRCT. However, these findings should be considered exploratory and require further confirmation in larger prospective studies. FAU - La Blasca, Tiziana AU - La Blasca T AD - Division of Respiratory Medicine, Department PROMISE "Paolo Giaccone" University Hospital, 90143 Palermo, Italy. FAU - Monteleone, Giorgio AU - Monteleone G AUID- ORCID: 0000-0001-7446-3209 AD - Division of Respiratory Medicine, Department PROMISE "Paolo Giaccone" University Hospital, 90143 Palermo, Italy. AD - Center for Interstitial and Rare Lung Diseases, Department of Pneumology, Ruhrlandklinik, University Hospital Essen, University of Duisburg-Essen, 45239 Essen, Germany. FAU - Di Liberti, Rosangela AU - Di Liberti R AD - Division of Respiratory Medicine, Department PROMISE "Paolo Giaccone" University Hospital, 90143 Palermo, Italy. FAU - Barbata, Giacomo AU - Barbata G AD - Division of Respiratory Medicine, Department PROMISE "Paolo Giaccone" University Hospital, 90143 Palermo, Italy. FAU - Lo Turco, Vittorio AU - Lo Turco V AD - Division of Respiratory Medicine, Department PROMISE "Paolo Giaccone" University Hospital, 90143 Palermo, Italy. FAU - La Barbera, Lidia AU - La Barbera L AD - Rheumatology Section, Department PROMISE, "Paolo Giaccone" University Hospital, 90143 Palermo, Italy. FAU - Guggino, Giuliana AU - Guggino G AD - Rheumatology Section, Department PROMISE, "Paolo Giaccone" University Hospital, 90143 Palermo, Italy. FAU - Messina, Riccardo AU - Messina R AD - Division of Respiratory Medicine, Department PROMISE "Paolo Giaccone" University Hospital, 90143 Palermo, Italy. FAU - Scichilone, Nicola AU - Scichilone N AUID- ORCID: 0000-0001-6400-6573 AD - Division of Respiratory Medicine, Department PROMISE "Paolo Giaccone" University Hospital, 90143 Palermo, Italy. LA - eng PT - Journal Article DEP - 20261008 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701566 OTO - NOTNLM OT - LUS score OT - disease activity score 28-C reactive protein OT - interstitial lung disease OT - lung ultrasound OT - rheumatoid arthritis OT - usual interstitial pneumonia pattern COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:35 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - biomedicines14102281 [pii] AID - biomedicines-14-02281 [pii] AID - 10.3390/biomedicines14102281 [doi] PST - epublish SO - Biomedicines. 2026 Oct 8;14(10):2281. doi: 10.3390/biomedicines14102281. PMID- 42940086 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 8 TI - The Impact of Idiopathic Pulmonary Fibrosis on the Incidence and Severity of Radiation Pneumonitis in Non-Small Cell Lung Cancer Patients Following Radiotherapy. LID - 10.3390/biomedicines14102280 [doi] LID - 2280 AB - Background/Objectives: Idiopathic pulmonary fibrosis (IPF) is known to increase the risk of severe pulmonary toxicity in non-small cell lung cancer (NSCLC) patients undergoing radiotherapy. This study aimed to evaluate the impact of comorbid IPF on the incidence and severity of radiation pneumonitis (RP) and overall survival (OS). Methods: This retrospective cohort study included NSCLC patients who received radiotherapy to the lung between 2015 and 2025. Patients were stratified into an IPF-comorbid group and a non-IPF control group. To minimize selection bias, variable-ratio (up to 1:2) propensity score matching with a caliper of 0.2 SD and survey-weighted analyses were utilized. Results: After matching, 196 patients (72 with IPF, 124 without IPF) were analyzed. The IPF-comorbid group had a significantly higher risk of any-grade RP (OR = 2.75, p = 0.001) and grade >/= 2 RP (OR = 2.87, p = 0.002). In multivariable analysis, comorbid IPF was independently associated with any-grade RP (adjusted OR = 3.55, p = 0.001) and grade >/= 2 RP (adjusted OR = 3.79, p = 0.001). With death as a competing event, the 6-month cumulative incidence of RP was 51.8% vs. 25.2%. IPF was independently associated with worse OS (adjusted HR = 2.51, p = 0.006), with a lower 3-year OS rate (44.4% vs. 68.1%). Conclusions: Comorbid IPF was independently associated with an increased risk of RP and overall mortality in NSCLC patients receiving radiotherapy. Because the cumulative incidence of RP diverged within the first months after radiotherapy, close multidisciplinary monitoring is warranted during this early period. FAU - Oh, Byung Wook AU - Oh BW AUID- ORCID: 0009-0004-5643-2694 AD - Department of Internal Medicine, Division of Pulmonology and Critical Care Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, Republic of Korea. AD - Department of Medicine, College of Medicine, Inje University, Gimhae 50834, Republic of Korea. FAU - Kim, Hye Min AU - Kim HM AD - Department of Internal Medicine, Division of Pulmonology and Critical Care Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, Republic of Korea. FAU - Lee, Geun In AU - Lee GI AD - Department of Internal Medicine, Division of Pulmonology and Critical Care Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, Republic of Korea. FAU - Yong, Seung Hyun AU - Yong SH AUID- ORCID: 0000-0002-1949-4798 AD - Department of Internal Medicine, Division of Pulmonology and Critical Care Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, Republic of Korea. FAU - Woo, A La AU - Woo A AD - Department of Internal Medicine, Division of Pulmonology and Critical Care Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, Republic of Korea. FAU - Kim, Eun Young AU - Kim EY AUID- ORCID: 0000-0002-3281-5744 AD - Department of Internal Medicine, Division of Pulmonology and Critical Care Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, Republic of Korea. FAU - Lee, Sang Hoon AU - Lee SH AD - Department of Internal Medicine, Division of Pulmonology and Critical Care Medicine, Severance Hospital, Yonsei University College of Medicine, Seoul 03722, Republic of Korea. LA - eng GR - 6-2025-0156/Yonsei University/ PT - Journal Article DEP - 20261008 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701241 OTO - NOTNLM OT - idiopathic pulmonary fibrosis OT - non-small cell lung cancer OT - radiation pneumonitis OT - radiotherapy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:35 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - biomedicines14102280 [pii] AID - biomedicines-14-02280 [pii] AID - 10.3390/biomedicines14102280 [doi] PST - epublish SO - Biomedicines. 2026 Oct 8;14(10):2280. doi: 10.3390/biomedicines14102280. PMID- 42940081 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 8 TI - Immunophenotypic Features of T-Lymphocyte Distribution Across Histological and Molecular Subtypes of Serous Ovarian Neoplasms: A Retrospective Immunohistochemical Study. LID - 10.3390/biomedicines14102275 [doi] LID - 2275 AB - Background/Objectives: Serous ovarian neoplasms comprise biologically distinct entities with substantial differences in clinical behavior and molecular characteristics. Comparative data on the quantitative and spatial distribution of T-cell populations across the spectrum of serous ovarian neoplasms, particularly borderline ovarian tumors (BOT) and serous cystadenomas (CA), remain limited. The aim of this study was to compare the quantitative and spatial distribution of CD3+, CD4+, CD8+, and FOXP3+ cells across serous ovarian neoplasms and to assess their associations with selected molecular characteristics. Methods: This retrospective, single-center observational study included 227 patients with high-grade serous carcinoma (HGSC; n = 108), low-grade serous carcinoma (LGSC; n = 49), BOT (n = 16), and CA (n = 54). The primary endpoint was the density of CD3+, CD4+, CD8+ and FOXP3+ immunopositive cells per mm(2), evaluated by immunohistochemistry and digital morphometry. Spatial distribution, differences across early pT substages, the CD8+/FOXP3+ ratio, and associations with selected molecular characteristics were additionally assessed. Age-adjusted generalized linear models were used to evaluate whether differences among histological groups were independent of patient age. Results: Compared with CA, HGSC showed an 18.58-fold higher CD3+ cell density and a 9.07-fold higher FOXP3+ cell density (both p < 0.001). Histological type remained significantly associated with CD3+, CD4+, CD8+, and FOXP3+ cell densities after adjustment for patient age (all p < 0.001), whereas age itself was not independently associated with any of the four markers (all p > 0.05). CD3+ cells were predominantly distributed in peritumoral areas, whereas HGSC also demonstrated diffuse intratumoral and stromal infiltration. Comparatively high T-cell densities were observed in BRCA1/2-mutated HGSC and HRD-positive subgroups. No statistically significant differences in immunopositive cell densities were detected across the analyzed pT1 substages. HGSC showed the lowest CD8+/FOXP3+ ratio (Me 1.18 [1.06-1.34]). Conclusions: Serous ovarian neoplasms demonstrate distinct histology-associated patterns of T-cell infiltration and spatial distribution, with additional heterogeneity across selected molecular subgroups. These differences remained evident after adjustment for patient age. Their functional and prognostic significance requires further validation using expanded immunophenotypic panels, multiplex approaches, and longitudinal clinical outcomes. FAU - Demyashkin, Grigory AU - Demyashkin G AD - Department of Digital Oncomorphology, National Medical Research Centre of Radiology, 2nd Botkinsky Pass., 3, 125284 Moscow, Russia. AD - Laboratory of Histology and Immunohistochemistry, Institute of Translational Medicine and Biotechnology, I.M. Sechenov First Moscow State Medical University (Sechenov University), Trubetskaya St., 8/2, 119048 Moscow, Russia. FAU - Dorfman, Sofia AU - Dorfman S AD - Department of Digital Oncomorphology, National Medical Research Centre of Radiology, 2nd Botkinsky Pass., 3, 125284 Moscow, Russia. FAU - Asaturova, Aleksandra AU - Asaturova A AUID- ORCID: 0000-0001-8739-5209 AD - National Medical Research Center for Obstetrics, Gynecology and Perinatology Named After Academician V.I. Kulakov, Oparina St., 4, 117513 Moscow, Russia. FAU - Rogozhina, Aleksandra AU - Rogozhina A AUID- ORCID: 0009-0009-6244-1219 AD - National Medical Research Center for Obstetrics, Gynecology and Perinatology Named After Academician V.I. Kulakov, Oparina St., 4, 117513 Moscow, Russia. FAU - Badlaeva, Alina AU - Badlaeva A AUID- ORCID: 0000-0001-5223-9767 AD - National Medical Research Center for Obstetrics, Gynecology and Perinatology Named After Academician V.I. Kulakov, Oparina St., 4, 117513 Moscow, Russia. FAU - Holdeenko, Vladislav AU - Holdeenko V AUID- ORCID: 0009-0004-7363-4394 AD - Research and Educational Resource Center for Immunophenotyping, Digital Spatial Profiling and Ultrastructural Analysis Innovative Technologies, Peoples' Friendship University of Russia (RUDN University), Miklukho-Maklaya St., 6, 117198 Moscow, Russia. FAU - Shchekin, Vladimir AU - Shchekin V AUID- ORCID: 0000-0003-3763-7454 AD - Department of Digital Oncomorphology, National Medical Research Centre of Radiology, 2nd Botkinsky Pass., 3, 125284 Moscow, Russia. FAU - Kulchenko, Nina AU - Kulchenko N AUID- ORCID: 0000-0002-4468-3670 AD - Research and Educational Resource Center for Immunophenotyping, Digital Spatial Profiling and Ultrastructural Analysis Innovative Technologies, Peoples' Friendship University of Russia (RUDN University), Miklukho-Maklaya St., 6, 117198 Moscow, Russia. FAU - Kononenko, Tamara AU - Kononenko T AD - Department of Digital Oncomorphology, National Medical Research Centre of Radiology, 2nd Botkinsky Pass., 3, 125284 Moscow, Russia. FAU - Vadyukhin, Matvey AU - Vadyukhin M AUID- ORCID: 0000-0002-6235-1020 AD - Department of Digital Oncomorphology, National Medical Research Centre of Radiology, 2nd Botkinsky Pass., 3, 125284 Moscow, Russia. FAU - Amirov, Nazim AU - Amirov N AD - Department of Digital Oncomorphology, National Medical Research Centre of Radiology, 2nd Botkinsky Pass., 3, 125284 Moscow, Russia. FAU - Borovaya, Tatyana AU - Borovaya T AD - Department of Digital Oncomorphology, National Medical Research Centre of Radiology, 2nd Botkinsky Pass., 3, 125284 Moscow, Russia. FAU - Shegai, Petr AU - Shegai P AD - Department of Digital Oncomorphology, National Medical Research Centre of Radiology, 2nd Botkinsky Pass., 3, 125284 Moscow, Russia. AD - Department of Urology and Operative Nephrology, Peoples' Friendship University of Russia (RUDN University), Miklukho-Maklaya Str., 6, 117198 Moscow, Russia. FAU - Kaprin, Andrey AU - Kaprin A AD - Department of Digital Oncomorphology, National Medical Research Centre of Radiology, 2nd Botkinsky Pass., 3, 125284 Moscow, Russia. AD - Department of Urology and Operative Nephrology, Peoples' Friendship University of Russia (RUDN University), Miklukho-Maklaya Str., 6, 117198 Moscow, Russia. LA - eng PT - Journal Article DEP - 20261008 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701458 OTO - NOTNLM OT - CD3 OT - CD4 OT - CD8 OT - FOXP3 OT - T lymphocytes OT - immunohistochemistry OT - ovarian cancer OT - serous ovarian tumors COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:35 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - biomedicines14102275 [pii] AID - biomedicines-14-02275 [pii] AID - 10.3390/biomedicines14102275 [doi] PST - epublish SO - Biomedicines. 2026 Oct 8;14(10):2275. doi: 10.3390/biomedicines14102275. PMID- 42940075 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 7 TI - BMP-2 in Orthopedic Trauma and Limb Reconstruction: Current Evidence, Limitations, and Future Directions. LID - 10.3390/biomedicines14102267 [doi] LID - 2267 AB - Background/Objectives: Recombinant human bone morphogenetic protein-2 (rhBMP-2) is an osteoinductive agent increasingly used in many orthopedic cases such as fracture healing, nonunion, critical-sized bone defects, and limb reconstruction cases. However, its efficacy and safety remain controversial especially regarding its optimal dosing, delivery, and adverse effect concerns. This narrative review aims to summarize the rhBMP-2 evidence in orthopedic trauma and limb reconstruction. Methods: A literature search was conducted on PubMed/MEDLINE, Scopus, and Web of Science up to August 2026. We prioritized meta-analyses, systematic reviews, randomized controlled trials, and clinical studies. Results: rhBMP-2 induces osteogenic differentiation, endochondral ossification, and bone regeneration through canonical Smad-dependent and non-canonical pathways. Current evidence suggests a potential use of rhBMP-2 as an adjunct in selected open fractures, delayed union and nonunion, and complex reconstruction patients, although the results are still heterogeneous. Some studies reported fewer secondary interventions in open tibial fractures, while others failed to reproduce a consistent advantage over standard care or autograft. Hydroxyapatite-based delivery systems have recently demonstrated promising union rates at lower total rhBMP-2 doses, suggesting that the carrier and delivery method may determine the effect. Currently available studies carry clear weaknesses: small samples, few adequately powered trials, heterogeneous indications, no established optimal dose, high cost, and complications that include inflammation and ectopic bone formation. Controlled-release scaffolds, heparin-based microparticles, biomimetic carriers, and gene- or mRNA-based delivery are future research directions currently under investigation. Conclusions: rhBMP-2 is a promising adjunct that may benefit selected high-risk patients, but it does not replace stable fixation, infection control, soft-tissue management, or autologous grafting. Further trials are needed to define appropriate patient selection, the minimum effective dose, and optimal delivery systems. FAU - Junaidi, Muhammad Ade AU - Junaidi MA AUID- ORCID: 0000-0003-4779-8227 AD - Trauma Division, Department of Orthopedics and Traumatology, Dr. Cipto Mangunkusumo Hospital, Faculty of Medicine Universitas Indonesia, Jakarta Pusat 10430, Indonesia. FAU - Budhy, Filberto AU - Budhy F AUID- ORCID: 0000-0003-4768-4000 AD - Department of Orthopedics and Traumatology, Dr. Cipto Mangunkusumo Hospital, Faculty of Medicine Universitas Indonesia, Jakarta Pusat 10430, Indonesia. FAU - Hakam, Mulki AU - Hakam M AD - Department of Orthopedics and Traumatology, Dr. Cipto Mangunkusumo Hospital, Faculty of Medicine Universitas Indonesia, Jakarta Pusat 10430, Indonesia. FAU - Amin, Bany Faris AU - Amin BF AD - Department of Orthopedics and Traumatology, Dr. Cipto Mangunkusumo Hospital, Faculty of Medicine Universitas Indonesia, Jakarta Pusat 10430, Indonesia. FAU - Baskoro, Muhammad Orri AU - Baskoro MO AUID- ORCID: 0009-0006-5706-6520 AD - Department of Orthopedics and Traumatology, Dr. Cipto Mangunkusumo Hospital, Faculty of Medicine Universitas Indonesia, Jakarta Pusat 10430, Indonesia. LA - eng PT - Journal Article PT - Review DEP - 20261007 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701960 OTO - NOTNLM OT - BMP-2 OT - bone defect OT - bone morphogenetic protein-2 OT - bone regeneration OT - fracture healing OT - limb reconstruction OT - orthopedic trauma COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/07 CRDT- 2026/10/27 10:35 PHST- 2026/09/20 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - biomedicines14102267 [pii] AID - biomedicines-14-02267 [pii] AID - 10.3390/biomedicines14102267 [doi] PST - epublish SO - Biomedicines. 2026 Oct 7;14(10):2267. doi: 10.3390/biomedicines14102267. PMID- 42940065 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 6 TI - Enhanced Antileukemic Efficacy of Venetoclax-Loaded PEG-PLGA Nanoparticles Against THP-1 Acute Monocytic Leukemia Cells via Suppression of BCL-2 and Pro-Survival Signaling Pathways. LID - 10.3390/biomedicines14102259 [doi] LID - 2259 AB - Background: Acute myeloid leukemia (AML) presents a significant therapeutic challenge due to the systemic toxicity associated with traditional chemotherapy. Venetoclax, a selective BCL-2 inhibitor, has shown promising antileukemic activity; however, limited pharmacokinetic properties impair its clinical use. Polymeric nanocarriers composed of PEG-PLGA provide a biocompatible delivery system that enhances drug stability, controls drug release kinetics, and increases intracellular drug concentration. Methods: The physicochemical characteristics of VEN-PEG-PLGA nanoparticles were assessed using dynamic light scattering (DLS), scanning electron microscopy (SEM), drug loading analysis, and in vitro drug release studies. The antileukemic potential was evaluated in THP-1 cells using the CCK-8 viability assay, cell cycle profiling, Annexin V-FITC/PI apoptosis quantification, and Western blot analysis of Bcl-2, cleaved caspase-3, cleaved caspase-9, ERK1/2, phospho-ERK1/2 (p-ERK1/2), NF-kB, and phospho-NF-kB (p-NF-kB). Results: The VEN-PEG-PLGA nanoparticles displayed an average particle size of 186 +/- 4 nm, polydispersity index of 0.195 +/- 0.005 with loading efficiency of 89%, and loading capacity of 6.26%. These nanoparticles exhibited a pH-sensitive drug release profile, with approximately 72-74% cumulative release at pH 7.4 and 88-89% at pH 5.5 after 96 h. In THP-1 cells. Both free venetoclax and VEN-PEG-PLGA nanoparticles exhibited concentration-dependent cytotoxicity, although free venetoclax was more cytotoxic with EC50 values of 5.59 x 10(-7) M (95% CI: 1.27 x 10(-7)-2.46 x 10(-6) M) and 1.35 x 10(-6) M (95% CI: 2.64 x 10(-7)-6.96 x 10(-6) M) for the free drug and nanoparticle form, respectively. Cell cycle analysis indicated an increase in the G0/G1 cell population in the control group following treatment with VEN-PEG-PLGA nanoparticles, along with a reduction in the G2/M phase population. Annexin V-FITC/PI analysis demonstrated that VEN-PEG-PLGA nanoparticles increased the ratio of late apoptotic cells, compared with free venetoclax treatment. Western blot analysis showed decreased expression of Bcl-2, p-ERK1/2, and p-NF-kB, along with increased levels of cleaved caspase-3 and cleaved caspase-9 following treatment, with more pronounced changes observed in the group treated with VEN-loaded PEG-PLGA nanoparticles. Conclusions: VEN-PEG-PLGA nanoparticles modulate BCL-2/ERK1/2/NF-kappaB-related apoptotic responses, cell cycle kinetics, and signaling pathways in THP-1 AML cells, providing in vitro evidence supporting preclinical research on this nanoformulation in the context of AML. FAU - Keni Begendi, Nermin AU - Keni Begendi N AUID- ORCID: 0000-0002-7570-1552 AD - Division of Hematology, Department of Internal Medicine, School of Medicine, Afyon Health Sciences University, Afyonkarahisar 03030, Turkiye. FAU - Kaga, Elif AU - Kaga E AUID- ORCID: 0000-0002-2279-6105 AD - Department of Biochemistry, Faculty of Pharmacy, Afyon Health Sciences University, Afyonkarahisar 03030, Turkiye. LA - eng GR - 26.GENEL.008/Afyonkarahisar Health Sciences University/ PT - Journal Article DEP - 20261006 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701444 OTO - NOTNLM OT - BCL-2 inhibition OT - ERK1/2 signaling OT - NF-kappaB pathway OT - PEG-PLGA nanoparticles OT - acute myeloid leukemia OT - apoptosis OT - drug delivery OT - nanomedicine OT - venetoclax COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/06 CRDT- 2026/10/27 10:35 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/06 00:00 [pmc-release] AID - biomedicines14102259 [pii] AID - biomedicines-14-02259 [pii] AID - 10.3390/biomedicines14102259 [doi] PST - epublish SO - Biomedicines. 2026 Oct 6;14(10):2259. doi: 10.3390/biomedicines14102259. PMID- 42940064 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 6 TI - Metabolic Rewiring of GPC3-Targeted CAR T Cells Expressing IL-15 and HER2-Targeted CAR T Cells via the ADA1-CD26 Axis Promotes Tumor Infiltration and Limits Systemic Toxicities. LID - 10.3390/biomedicines14102258 [doi] LID - 2258 AB - Background: Chimeric antigen receptor (CAR) T-cell therapy for solid tumors is limited by inadequate T-cell persistence and tumor infiltration, as well as treatment-associated cytokine release syndrome (CRS). To address these challenges, we developed a metabolically rewired CAR T-cell (MRCAR) platform by co-expressing adenosine deaminase 1 (ADA1) and CD26 in CAR T cells. Methods: MRCAR T cells were generated using either GPC3-targeted CARs for hepatocellular carcinoma (HCC) or HER2-targeted CARs for breast cancer. For HCC, we further evaluated constructs with and without enforced IL-15 expression to boost T-cell function. Results: In vitro, IL-15-expressing GPC3-MRCAR T cells exhibited enhanced proliferation, sustained effector function, reduced exhaustion, and superior cytotoxicity following repeated antigen stimulation compared with conventional CAR T cells. CD26 expression significantly enhanced CAR T-cell invasiveness in transwell assays and promoted tumor infiltration in vivo in both tumor models. ADA1 expression enhanced CAR T-cell persistence and was associated with increased frequencies of memory and stem-like T-cell populations in vivo. Notably, although IL15-GPC3-CAR T-cell treatment increased antitumor activity, it also caused high mortality and systemic inflammation in Huh7 tumor-bearing mice, whereas incorporation of ADA1 and CD26 into MRCAR T cells significantly improved survival and reduced serum levels of IL-6, IFN-gamma, and TNF-alpha. Mechanistically, conventional IL-15-expressing GPC3-CAR T cells induced proinflammatory M1-like macrophage activation, whereas MRCAR T cells markedly suppressed this inflammatory response. Conclusions: Collectively, these results indicate that metabolic rewiring via ADA1 and CD26 enhances CAR T-cell expansion, tumor infiltration, persistence, and safety, supporting the MRCAR platform as a promising strategy to improve both efficacy and tolerability of CAR T-cell therapy for solid tumors. FAU - Hu, Yue AU - Hu Y AUID- ORCID: 0009-0005-2192-846X AD - Center for Inflammatory and Infectious Diseases, Institute of Biosciences and Technology, Texas A&M University, Houston, TX 77030, USA. AD - Department of Translational Medical Sciences, Texas A&M University, Houston, TX 77030, USA. FAU - Song, Kevin AU - Song K AD - Center for Inflammatory and Infectious Diseases, Institute of Biosciences and Technology, Texas A&M University, Houston, TX 77030, USA. AD - Department of Translational Medical Sciences, Texas A&M University, Houston, TX 77030, USA. AD - Department of Biology, University of Houston, Houston, TX 77004, USA. FAU - Wang, Ruoning AU - Wang R AD - Center for Childhood Cancer Research, Nationwide Children's Hospital, Columbus, OH 43205, USA. AD - Hematology, Oncology & Blodd and Marrow Transplant, Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH 43205, USA. AD - Department of Pediatrics, The Ohio State University, Columbus, OH 43205, USA. FAU - Gottschalk, Stephen AU - Gottschalk S AUID- ORCID: 0000-0003-3991-7468 AD - Department of Bone Marrow Transplantation & Cellular Therapy, St. Jude Children's Research Hospital, Memphis, TN 38105, USA. FAU - Heczey, Andras AU - Heczey A AUID- ORCID: 0000-0002-4263-9115 AD - Hematology-Oncology, Seattle Children's Hospital, University of Washington School of Medicine, Seattle, WA 98105, USA. FAU - Song, Xiaotong AU - Song X AUID- ORCID: 0000-0002-2907-2898 AD - Center for Inflammatory and Infectious Diseases, Institute of Biosciences and Technology, Texas A&M University, Houston, TX 77030, USA. AD - Department of Translational Medical Sciences, Texas A&M University, Houston, TX 77030, USA. LA - eng GR - P30 CA125123/CA/NCI NIH HHS/United States GR - R01 AI175004/AI/NIAID NIH HHS/United States GR - R01 CA299046/CA/NCI NIH HHS/United States GR - R37 AI114581/AI/NIAID NIH HHS/United States PT - Journal Article DEP - 20261006 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701173 OTO - NOTNLM OT - ADA1 OT - CAR T cell OT - CD26 OT - IL-15 OT - cytokine release syndrome OT - inflammation OT - invasion OT - macrophage polarization OT - memory T cells OT - persistence OT - solid tumor COIS- X.S. is the founder of Cellula BioPharma, Inc., which holds a licensing agreement with Texas A&M University related to technology described in this article. X.S., R.W. and Y.H. are co-inventors of this related technology. S.G. is a co-inventor on patent applications and patents in the fields of cell and gene therapy for cancer. During the past two years, he has served as a member of the Data and Safety Monitoring Board of Immatics and on the Scientific Advisory Boards of Be Biopharma and Tikva Allocell. The other authors declare no competing interests. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/06 CRDT- 2026/10/27 10:35 PHST- 2026/09/04 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/06 00:00 [pmc-release] AID - biomedicines14102258 [pii] AID - biomedicines-14-02258 [pii] AID - 10.3390/biomedicines14102258 [doi] PST - epublish SO - Biomedicines. 2026 Oct 6;14(10):2258. doi: 10.3390/biomedicines14102258. PMID- 42940061 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 6 TI - Obesity and Oral Health: Clinical Associations, Mechanistic Evidence, and Implications for Dental Care-A Narrative Review. LID - 10.3390/biomedicines14102255 [doi] LID - 2255 AB - Obesity is a heterogeneous multisystem condition characterized by chronic low-grade inflammation, oxidative stress, adipokine dysregulation, and insulin resistance; its effects on oral microbial homeostasis, tissue regeneration, and disease susceptibility vary with metabolic context and with the outcome examined. This narrative review synthesizes evidence on the mechanisms linking obesity to oral disease and on its implications for dental prevention, periodontal care, implant therapy, and oral surgery. MEDLINE via PubMed, Scopus, and Web of Science Core Collection were searched from January 2000 to 31 August 2026, with the final search performed on 10 September 2026. The strength of evidence differs markedly by outcome. Human epidemiological studies support an association with periodontitis; altered salivary flow and composition are reported mainly in cross-sectional studies. Proposed effects on osseous healing and jawbone remodeling rest substantially on experimental and systemic bone research, and specific marrow alterations remain poorly established. Obesity is associated with selected peri-implant inflammatory outcomes, whereas evidence on implant survival is less consistent; these findings establish neither impaired primary osseointegration nor a contraindication to implant therapy. Associations with caries appear largely attributable to shared determinants. The phenotype-aware framework proposed here is hypothesis-generating and requires prospective interventional validation. FAU - Dominiak, Marzena AU - Dominiak M AD - Department of Dental Surgery, Faculty of Dentistry, Wroclaw Medical University, Krakowska 26, 50-425 Wroclaw, Poland. FAU - Niemczyk, Wojciech AU - Niemczyk W AD - Department of Dental Surgery, Faculty of Dentistry, Wroclaw Medical University, Krakowska 26, 50-425 Wroclaw, Poland. FAU - Suwala, Szymon AU - Suwala S AUID- ORCID: 0000-0002-5865-8484 AD - Department of Endocrinology and Diabetology, Faculty of Medicine, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Sklodowskiej-Curie 9, 85-094 Bydgoszcz, Poland. AD - Clinic of Metabolic Rehabilitation, 22nd Military Hospital of Spa and Rehabilitation in Ciechocinek, Wojska Polskiego 5, 87-720 Ciechocinek, Poland. FAU - Gedrange, Tomasz AU - Gedrange T AD - Department of Dental Surgery, Faculty of Dentistry, Wroclaw Medical University, Krakowska 26, 50-425 Wroclaw, Poland. FAU - Geller, Victor AU - Geller V AUID- ORCID: 0009-0003-0446-6721 AD - Department of Dental Surgery, Faculty of Dentistry, Wroclaw Medical University, Krakowska 26, 50-425 Wroclaw, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261006 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701933 OTO - NOTNLM OT - adipokines OT - insulin resistance OT - jawbone OT - metabolic syndrome OT - obesity OT - oral health OT - oral microbiome OT - peri-implantitis OT - periodontitis OT - wound healing COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/06 CRDT- 2026/10/27 10:35 PHST- 2026/08/07 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/06 00:00 [pmc-release] AID - biomedicines14102255 [pii] AID - biomedicines-14-02255 [pii] AID - 10.3390/biomedicines14102255 [doi] PST - epublish SO - Biomedicines. 2026 Oct 6;14(10):2255. doi: 10.3390/biomedicines14102255. PMID- 42940059 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 5 TI - Artificial Intelligence, Multi-Omics, and Multimodal Integration in Precision Radiotherapy for Head and Neck Cancer: From Biological Insights to Treatment Decisions. LID - 10.3390/biomedicines14102252 [doi] LID - 2252 AB - Radiotherapy for head and neck cancer must balance disease control against normal-tissue injury. Artificial intelligence (AI), multi-omics, and multimodal analysis offer complementary ways to characterize tumor and host heterogeneity, but their relevance to treatment decisions depends on how measurements, outcomes, and validation are linked. This narrative review examines these approaches across head and neck cancers, with evidence concentrated in head and neck squamous cell carcinoma and nasopharyngeal carcinoma. Literature was identified through Web of Science and PubMed searches with targeted supplementary searches and reference-list screening, and selected for relevance to radiotherapy biology, clinical outcomes, and methodological or clinical validation. We distinguish joint molecular or multimodal inputs from parallel analyses and post hoc biological annotation, and assess findings according to population, treatment setting, sampling time, and analytical unit. Genomic, proteogenomic, single-cell, and spatial studies identify candidate phenotypes involving deoxyribonucleic acid (DNA) repair, viral regulation, immunity, and metabolism. Clinical studies examine response, recurrence and survival stratification, molecular surveillance, and toxicity prediction. However, associations with outcomes under observed treatment do not establish differential treatment benefit, and improved discrimination does not demonstrate that model-guided care improves patient outcomes. Small patient cohorts, incomplete assay availability, potential data leakage, and limited independent validation further constrain interpretation. Translation requires reproducible measurements, patient-level validation of complete analytical pipelines, calibrated risk estimates, and evaluation within the intended treatment context. Prospective evaluation should prioritize predefined model-guided strategies and assess disease control, patient-relevant harm, and practical implementation together. FAU - Rao, Miao AU - Rao M AD - Guangxi Medical University Cancer Hospital, Nanning 530000, China. FAU - Wang, Yingjian AU - Wang Y AD - Department of Urology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. FAU - Li, Minglun AU - Li M AUID- ORCID: 0000-0002-7312-8350 AD - Department of Radiation Oncology, Klinikum Luneburg, 21339 Luneburg, Germany. FAU - Shi, Run AU - Shi R AD - Brain Metastases Diagnosis and Treatment Center, The Affiliated Brain Hospital of Nanjing Medical University, Nanjing 210029, China. LA - eng PT - Journal Article PT - Review DEP - 20261005 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701673 OTO - NOTNLM OT - artificial intelligence OT - biomarkers OT - clinical validation OT - head and neck cancer OT - multi-omics OT - multimodal integration OT - precision radiotherapy OT - treatment selection COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/05 CRDT- 2026/10/27 10:35 PHST- 2026/09/12 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/05 00:00 [pmc-release] AID - biomedicines14102252 [pii] AID - biomedicines-14-02252 [pii] AID - 10.3390/biomedicines14102252 [doi] PST - epublish SO - Biomedicines. 2026 Oct 5;14(10):2252. doi: 10.3390/biomedicines14102252. PMID- 42940057 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 5 TI - Focused Ultrasound-Mediated Blood-Brain Barrier Opening for Enhanced Drug Delivery in Diffuse Gliomas: A Systematic Review. LID - 10.3390/biomedicines14102251 [doi] LID - 2251 AB - Background/Objectives: The blood-brain barrier (BBB) severely limits the intratumoral delivery of systemically administered therapeutics in diffuse gliomas, including glioblastoma (GBM), contributing to the persistently poor prognosis of these tumors. Focused ultrasound (FUS) combined with microbubble agents enables transient, spatially targeted, and reversible BBB opening (BBBO), offering a non-invasive strategy to enhance drug delivery in this setting. Despite a rapidly growing body of clinical evidence, a systematic synthesis of outcomes across FUS platforms, drug regimens, and study designs is currently lacking. This systematic review aims to critically appraise the available clinical evidence on FUS-mediated BBBO for enhanced drug delivery in adult patients with diffuse gliomas. Methods: A systematic search of PubMed/MEDLINE and Scopus was performed using pre-specified search terms (last search: June 2026). Eligible studies included clinical trials and cohort studies enrolling adult patients (>/=18 years) with histologically confirmed diffuse glioma (WHO grades II-IV) who underwent FUS-BBBO for therapeutic drug delivery enhancement. Two reviewers independently screened records and extracted data using a standardized form. Study quality was assessed using the NIH Quality Assessment Tool for Before-After Studies (single-arm studies) and the ROBINS-I tool (comparative studies). The review was retrospectively registered on OSF. Results were synthesized narratively due to study heterogeneity. Results: Eight publications from seven independent clinical studies were included. Across these publications, 68 patient entries were reported; however, because of overlapping populations across publications, the number of unique patients could not be precisely determined. Two FUS platforms were employed, with the ExAblate Neuro system (220-230 kHz) used in six out of seven cohorts. BBB opening was confirmed in all studies, with target sonication success rates of 92.4-100%. No procedure-related serious adverse events attributable to FUS-BBBO were reported across any study. Transient T2/SWAN hypointense foci were observed in a subset of patients, consistently asymptomatic and histologically benign. Direct intratumoral drug quantification was feasible in two pre-surgical studies, demonstrating a 2.2-fold increase in drug accumulation in FUS-treated versus untreated tissue (p < 0.01). The only comparative study reported a statistically significant improvement in overall survival (median 31.3 vs. 19.3 months; HR 0.50, p = 0.009) and progression-free survival (median 13.5 vs. 8.1 months; HR 0.55, p = 0.020) versus the controls. Conclusions: FUS-mediated BBBO is a safe and feasible approach for enhancing drug delivery in adult diffuse glioma patients across multiple platforms and drug combinations. Preliminary comparative data suggest a potential survival benefit in newly diagnosed GBM; however, these findings are based on a single non-randomized study and require confirmation in prospective randomized controlled trials. FAU - Ciaffi, Gabriele AU - Ciaffi G AD - Department of Neurosurgery, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy. FAU - Martinelli, Renata AU - Martinelli R AUID- ORCID: 0009-0008-0369-0656 AD - Department of Neurosurgery, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy. FAU - Traverso, Andrea AU - Traverso A AD - Department of Neurosurgery, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy. FAU - Burattini, Benedetta AU - Burattini B AUID- ORCID: 0000-0002-7823-1752 AD - Department of Neurosurgery, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy. FAU - Bruzzaniti, Placido AU - Bruzzaniti P AUID- ORCID: 0000-0003-1873-7687 AD - Department of Neurosurgery, San Carlo Borromeo Hospital, 20153 Milan, Italy. FAU - Papacci, Fabio AU - Papacci F AD - Department of Neurosurgery, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy. FAU - Talacchi, Andrea AU - Talacchi A AD - Azienda Ospedaliera San Giovanni Addolorata, Neurochirurgia, 00184 Rome, Italy. FAU - Della Pepa, Giuseppe Maria AU - Della Pepa GM AD - Department of Neurosurgery, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy. FAU - Pennisi, Giovanni AU - Pennisi G AUID- ORCID: 0000-0003-3595-2418 AD - Azienda Ospedaliera San Giovanni Addolorata, Neurochirurgia, 00184 Rome, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261005 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701403 OTO - NOTNLM OT - MRgFUS OT - blood-brain barrier opening OT - drug delivery OT - focused ultrasound OT - glioblastoma OT - glioma OT - microbubbles OT - neuro-oncology OT - systematic review COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/05 CRDT- 2026/10/27 10:35 PHST- 2026/09/05 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/05 00:00 [pmc-release] AID - biomedicines14102251 [pii] AID - biomedicines-14-02251 [pii] AID - 10.3390/biomedicines14102251 [doi] PST - epublish SO - Biomedicines. 2026 Oct 5;14(10):2251. doi: 10.3390/biomedicines14102251. PMID- 42940054 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 4 TI - Association of VCP/p97 Expression with Clinicopathological Features and Overall Survival in Endometrioid Endometrial Carcinoma. LID - 10.3390/biomedicines14102248 [doi] LID - 2248 AB - Background and Aim: Endometrial cancer is considered one of the most common cancers of the female genital system, with endometrioid endometrial carcinoma (EEC) being the most common subtype. Valosin-containing protein (VCP), or p97, acts as an important regulator of cellular protein homeostasis and is thought to play a role in the progression and prognosis of various tumors. This study aimed to evaluate the association of immunohistochemical VCP/p97 expression with clinicopathological features and overall survival in patients with EEC. Methods: This retrospective single-center study utilized hysterectomy specimens obtained from 85 patients diagnosed with EEC between January of 2010 and December of 2022. All cases were retrospectively restaged according to the 2023 FIGO staging system. VCP/p97 expression was evaluated immunohistochemically using a semi-quantitative scoring system based on staining intensity and the percentage of positive tumor cells. Patients with EEC were divided into groups with low and high VCP/p97 expression. Clinicopathological associations, alternative cut-off sensitivity analyses, Kaplan-Meier survival analysis, and Cox regression analyses were performed. Results: Low VCP/p97 expression was detected in 49 cases (57.6%), whereas high expression was detected in 36 cases (42.4%). High VCP/p97 expression was significantly associated with older age, larger tumor size, high FIGO grade, deep myometrial invasion, lymphovascular invasion, cervical and adnexal involvement, pathological lymph node metastasis, distant metastasis, and advanced FIGO stage. Overall survival was significantly worse in the high-expression group (log-rank p < 0.001). In the multivariate Cox regression analysis, high VCP/p97 expression remained associated with poorer overall survival. Conclusions: High immunohistochemical VCP/p97 expression is associated with an adverse clinicopathological phenotype and poorer overall survival in EEC. VCP/p97 may be a considered a candidate tissue-based marker associated with tumor progression features; however, its independent prognostic utility needs to be validated in larger independent cohorts. FAU - Arici, Akgul AU - Arici A AUID- ORCID: 0000-0002-7347-9003 AD - Department of Medical Pathology, School of Medicine, Tokat Gaziosmanpasa University, Tokat 60250, Turkiye. FAU - Akcay, Elif AU - Akcay E AUID- ORCID: 0000-0001-5055-5854 AD - Department of Medical Pathology, School of Medicine, Tokat Gaziosmanpasa University, Tokat 60250, Turkiye. FAU - Arslan, Kubra AU - Arslan K AUID- ORCID: 0000-0002-7399-3627 AD - Department of Medical Pathology, Gaziantep City Hospital, Gaziantep 27470, Turkiye. FAU - Demir, Busra AU - Demir B AUID- ORCID: 0000-0001-7279-5808 AD - Department of Medical Pathology, School of Medicine, Harran University, Sanliurfa 63300, Turkiye. FAU - Koseoglu, Resit Dogan AU - Koseoglu RD AUID- ORCID: 0000-0001-7389-6811 AD - Department of Medical Pathology, School of Medicine, Tokat Gaziosmanpasa University, Tokat 60250, Turkiye. LA - eng PT - Journal Article DEP - 20261004 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701805 OTO - NOTNLM OT - endometrial cancer OT - endometrioid endometrial carcinoma OT - immunohistochemistry OT - tumor progression OT - valosin-containing protein COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/04 CRDT- 2026/10/27 10:35 PHST- 2026/08/19 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/04 00:00 [pmc-release] AID - biomedicines14102248 [pii] AID - biomedicines-14-02248 [pii] AID - 10.3390/biomedicines14102248 [doi] PST - epublish SO - Biomedicines. 2026 Oct 4;14(10):2248. doi: 10.3390/biomedicines14102248. PMID- 42940051 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 4 TI - Diminazene as a Potential Pharmacological Candidate Against Tebuconazole-Induced Cardiotoxicity. LID - 10.3390/biomedicines14102245 [doi] LID - 2245 AB - Background/Objectives: Tebuconazole (TEB) is a fungicide whose exposure has been increasingly linked to adverse cardiovascular outcomes. However, no specific therapeutic approaches are available to counteract TEB-induced cardiotoxicity, underscoring the need for new pharmacological strategies. Diminazene (DIZE) has emerged as a promising cardioprotective agent through its modulatory effects on the renin-angiotensin system (RAS), particularly via activation of the ACE2/Ang-(1-7)/Mas receptor. Methods: This study investigated whether DIZE protects against TEB cardiotoxicity using an in vitro model utilizing H9c2 cardiomyoblasts and an ex vivo model employing Langendorff-perfused rat hearts. Results: In cardiomyoblasts, TEB induced a dose-dependent reduction in cell viability, accompanied by increased apoptotic/necrotic cell death. A concentration of 100 microM TEB, representing an intermediate toxicity profile, was selected for subsequent experiments. Co-exposure to DIZE (100-1000 microM) attenuated TEB-induced cytotoxicity in a concentration-dependent manner, restored cell viability and reduced oxidative and nitrosative stress markers, specifically reactive oxygen species (measured via DHE) and nitric oxide (via DAF-2 DA). Furthermore, TEB showed lower mean ACE2 expression and DIZE prevented the downregulation. In isolated heart preparations, TEB caused pronounced mechanical and electrophysiological dysfunction. Mechanically, TEB reduced left ventricular developed pressure, impaired contractility, increased end-diastolic pressure, and prolonged systolic duration. Electrophysiologically, TEB induced bradycardia, PR prolongation, and QT/QTc and T-wave prolongation, indicating delayed atrioventricular conduction and ventricular repolarization. DIZE markedly attenuated both the mechanical and electrical abnormalities induced by TEB. Conclusions: These findings provide the first experimental evidence of DIZE against TEB-induced cardiac injury and support further investigation of its potential against pesticide-induced cardiotoxicity. FAU - Resende, Ana Luiza Borges AU - Resende ALB AD - Faculdade de Ciencias Medicas de Minas Gerais, Belo Horizonte 30130-110, MG, Brazil. AD - Laboratorio de Investigacoes NeuroCardiacas, Ciencias Medicas de Minas Gerais (LINC CMMG), Belo Horizonte 30130-110, MG, Brazil. FAU - Martins, Fabiana Dos Santos AU - Martins FDS AD - Faculdade de Ciencias Medicas de Minas Gerais, Belo Horizonte 30130-110, MG, Brazil. AD - Laboratorio de Investigacoes NeuroCardiacas, Ciencias Medicas de Minas Gerais (LINC CMMG), Belo Horizonte 30130-110, MG, Brazil. FAU - Marques, Ivan Lobo de Sousa AU - Marques ILS AUID- ORCID: 0009-0004-8343-0088 AD - Department of Physiology and Biophysics, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil. FAU - Teles, Alessandra Silva AU - Teles AS AD - Department of Physiology and Biophysics, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil. FAU - de Almeida, Douglas Lamounier AU - de Almeida DL AUID- ORCID: 0000-0001-6632-7311 AD - Faculdade de Ciencias Medicas de Minas Gerais, Belo Horizonte 30130-110, MG, Brazil. AD - Laboratorio de Investigacoes NeuroCardiacas, Ciencias Medicas de Minas Gerais (LINC CMMG), Belo Horizonte 30130-110, MG, Brazil. AD - Post-Graduate Program in Health Sciences, Faculdade de Ciencias Medicas de Minas Gerais, Belo Horizonte 30130-110, MG, Brazil. FAU - Guimaraes, Antonio Rogerio Dias AU - Guimaraes ARD AD - Faculdade de Ciencias Medicas de Minas Gerais, Belo Horizonte 30130-110, MG, Brazil. AD - Laboratorio de Investigacoes NeuroCardiacas, Ciencias Medicas de Minas Gerais (LINC CMMG), Belo Horizonte 30130-110, MG, Brazil. AD - Post-Graduate Program in Health Sciences, Faculdade de Ciencias Medicas de Minas Gerais, Belo Horizonte 30130-110, MG, Brazil. AD - Laboratorio de Inovacao Tecnico-Cientifica, FELUMA-Ciencias Medicas de Minas Gerais, Belo Horizonte 30130-007, MG, Brazil. FAU - Santos-Miranda, Artur AU - Santos-Miranda A AD - Department of Physiology and Biophysics, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil. FAU - Nascimento, Daniella Santos AU - Nascimento DS AD - Department of Physiology, Universidade Federal de Sergipe, Aracaju 49107-230, SE, Brazil. FAU - Souza, Diego Santos de AU - Souza DS AUID- ORCID: 0000-0002-3508-9776 AD - Department of Physiology, Universidade Federal de Sergipe, Aracaju 49107-230, SE, Brazil. FAU - Kangussu, Lucas Miranda AU - Kangussu LM AUID- ORCID: 0000-0003-3678-118X AD - Department of Morphology, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil. FAU - Joviano-Santos, Julliane Vasconcelos AU - Joviano-Santos JV AUID- ORCID: 0000-0002-5788-6223 AD - Faculdade de Ciencias Medicas de Minas Gerais, Belo Horizonte 30130-110, MG, Brazil. AD - Laboratorio de Investigacoes NeuroCardiacas, Ciencias Medicas de Minas Gerais (LINC CMMG), Belo Horizonte 30130-110, MG, Brazil. AD - Post-Graduate Program in Health Sciences, Faculdade de Ciencias Medicas de Minas Gerais, Belo Horizonte 30130-110, MG, Brazil. LA - eng GR - APQ-01680-23/Fundacao de Amparo a Pesquisa do Estado de Minas Gerais/ GR - Lucas Machado Educational Foundation/ GR - Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/ GR - 406816/2022-0; #409449/2024-5 and #318573/2025-3/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ GR - APQ-05839-23/Instituto Serrapilheira/ PT - Journal Article DEP - 20261004 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701889 OTO - NOTNLM OT - cardioprotective drugs OT - cardiotoxicity OT - diminazene OT - heart OT - renin-angiotensin system OT - tebuconazole COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/04 CRDT- 2026/10/27 10:35 PHST- 2026/09/07 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/04 00:00 [pmc-release] AID - biomedicines14102245 [pii] AID - biomedicines-14-02245 [pii] AID - 10.3390/biomedicines14102245 [doi] PST - epublish SO - Biomedicines. 2026 Oct 4;14(10):2245. doi: 10.3390/biomedicines14102245. PMID- 42940047 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 3 TI - The Mitophagy Vortex: Bridging Inflammatory, Oxidative, Hypoxic, and Aging Insults to Drive COPD Skeletal Muscle Dysfunction. LID - 10.3390/biomedicines14102240 [doi] LID - 2240 AB - Skeletal muscle dysfunction is a frequent extrapulmonary complication of chronic obstructive pulmonary disease (COPD) that limits physical capacity and increases mortality. Systemic inflammation, oxidative stress, hypoxia, and aging are distinct in origin and signaling, yet each has been implicated in COPD muscle deterioration. Whether these four drivers converge on a common pathway remains unclear. This review proposes that mitophagy, the selective elimination of damaged mitochondria, serves as this hub. Rather than acting in isolation, the four drivers converge through two functionally distinct amplification axes. The inflammatory-oxidative axis escalates mitochondrial injury, while the hypoxic-aging axis progressively erodes lysosomal disposal capacity, lowering the threshold for quality-control failure. Once injury load outruns clearance capacity, retained damaged mitochondria release mtDNA and overproduce ROS, which amplify upstream inflammatory signals and further impair lysosomal function, a self-reinforcing disequilibrium in which each iteration amplifies the next. This architecture, which we term the mitophagy vortex, implies that the four factors do not add linearly but multiply through a common quality-control node, explaining why single-target interventions have consistently failed. The framework predicts that restoring lysosomal acidification or autophagic flux, rather than neutralizing individual cytokines or ROS, may carry greater therapeutic weight once the vortex is established. Targeting the mitophagy machinery at multiple levels, including its receptors, enzymes, and lysosomal function, represents a mechanistically coherent strategy for breaking the pathological cycle. FAU - Li, Jian AU - Li J AUID- ORCID: 0000-0002-9421-3116 AD - School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. FAU - Li, Peijun AU - Li P AUID- ORCID: 0000-0002-5303-009X AD - School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. FAU - Zhao, Xianli AU - Zhao X AD - School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. FAU - Jiang, Lele AU - Jiang L AD - School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. FAU - Li, Hui AU - Li H AD - School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. FAU - Chen, Fuying AU - Chen F AD - School of Exercise and Health, Shanghai University of Sport, Shanghai 200438, China. FAU - Chen, Cai-Tao AU - Chen CT AD - Department of Rehabilitation Medicine, Shanghai Fourth People's Hospital, School of Medicine, Tongji University, Shanghai 200434, China. FAU - Wu, Weibing AU - Wu W AD - School of Exercise and Health, Shanghai University of Sport, Shanghai 200438, China. FAU - Liu, Xiaodan AU - Liu X AD - School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. LA - eng GR - 82402964/National Natural Science Foundation of China/ PT - Journal Article PT - Review DEP - 20261003 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701502 OTO - NOTNLM OT - aging OT - chronic obstructive pulmonary disease OT - hypoxia OT - mitophagy OT - oxidative stress OT - skeletal muscle dysfunction OT - systemic inflammation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/03 CRDT- 2026/10/27 10:35 PHST- 2026/09/10 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - biomedicines14102240 [pii] AID - biomedicines-14-02240 [pii] AID - 10.3390/biomedicines14102240 [doi] PST - epublish SO - Biomedicines. 2026 Oct 3;14(10):2240. doi: 10.3390/biomedicines14102240. PMID- 42940046 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 3 TI - Integrative Multi-Omics Reveals TSPAN13 as a Candidate Epithelial-Mesenchymal Transition-Associated Regulator in Prostate Cancer. LID - 10.3390/biomedicines14102241 [doi] LID - 2241 AB - Background: Prostate cancer (PCa) is a malignancy with high metastatic potential and poor prognosis, underscoring the urgent need for effective biomarkers and therapeutic targets to improve clinical management. Methods: An integrative multi-omics analysis combined with machine learning algorithms was performed to identify key diagnostic biomarkers. Candidate genes were validated through extensive functional assays in vitro, including proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) analyses. The tumor-promoting effect of TSPAN13 was further assessed in vivo using xenograft mouse models. Immune cell infiltration patterns were analyzed to explore associations with the tumor microenvironment. Results: An exploratory diagnostic model centered on TSPAN13 was developed. Functional depletion of TSPAN13 significantly suppressed PCa cell proliferation, migration, invasion, and EMT in vitro. These anti-tumor effects were recapitulated in vivo, where TSPAN13 knockdown markedly inhibited tumor growth without significant change in body weight. Furthermore, TSPAN13 expression was correlated with distinct alterations in the immune cell composition within the tumor microenvironment. Conclusions: This study identifies TSPAN13 as a candidate EMT-associated regulator in PCa with potential diagnostic and therapeutic relevance. Our findings provide novel insights into PCa pathogenesis and highlight TSPAN13 as a potential candidate for preclinical exploration, offering potential for strategies targeting epithelial malignancy and modulating the immune microenvironment. FAU - Lu, Shuo AU - Lu S AD - Department of Urology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China. FAU - Li, Houhe AU - Li H AD - Department of Urology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China. FAU - Zhang, Zixiao AU - Zhang Z AD - Department of Urology, Zhaoqing Hospital, The Third Affiliated Hospital, Sun Yat-sen University, Zhaoqing 526071, China. FAU - Yu, Dafu AU - Yu D AD - Department of Urology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China. FAU - Qiao, Yuming AU - Qiao Y AD - Department of Urology, Zhaoqing Hospital, The Third Affiliated Hospital, Sun Yat-sen University, Zhaoqing 526071, China. FAU - Ouyang, Junyu AU - Ouyang J AD - Department of Urology, Zhaoqing Hospital, The Third Affiliated Hospital, Sun Yat-sen University, Zhaoqing 526071, China. FAU - Liu, Shaowen AU - Liu S AD - Department of Urology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China. FAU - Wang, Hua AU - Wang H AD - Department of Urology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China. FAU - Li, Ke AU - Li K AUID- ORCID: 0000-0002-0391-521X AD - Department of Urology, The Third Affiliated Hospital, Sun Yat-sen University, Guangzhou 510630, China. AD - Department of Urology, Zhaoqing Hospital, The Third Affiliated Hospital, Sun Yat-sen University, Zhaoqing 526071, China. LA - eng GR - Program No. 82272896/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261003 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701793 OTO - NOTNLM OT - epithelial-mesenchymal transition OT - machine learning OT - molecular dynamics OT - prostate cancer OT - single-cell RNA sequencing COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/03 CRDT- 2026/10/27 10:35 PHST- 2026/08/11 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - biomedicines14102241 [pii] AID - biomedicines-14-02241 [pii] AID - 10.3390/biomedicines14102241 [doi] PST - epublish SO - Biomedicines. 2026 Oct 3;14(10):2241. doi: 10.3390/biomedicines14102241. PMID- 42940044 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 3 TI - Predictors of Favourable Response to Cardiac Resynchronization Therapy in Patients with Heart Failure with Reduced Ejection Fraction: A Narrative Review. LID - 10.3390/biomedicines14102239 [doi] LID - 2239 AB - Cardiac resynchronization therapy (CRT) is an effective treatment for selected patients with heart failure with reduced ejection fraction, but 30-40% of recipients do not respond. This narrative review summarizes predictors of a favourable response to CRT. Typical left bundle branch block (LBBB) and a QRS duration >/= 150 ms remain the best-established baseline predictors. Sex, etiology, age and comorbidities affect how much benefit is gained rather than acting as yes/no predictors (the benefit is greater in women, partly because of their smaller heart size) and should not by themselves exclude an otherwise eligible patient. QRS duration indexed to left ventricular size, advanced electrocardiographic markers (QRS notching, intrinsicoid deflection, QRmax, QRS area) and echocardiographic markers of mechanical dyssynchrony (apical rocking, septal flash, the classical LBBB strain pattern, systolic stretch index, mechanical dispersion and myocardial work) have been linked to response in observational studies and may add prognostic information. Combining mechanical dyssynchrony with septal viability on cardiac magnetic resonance appears to improve selection further. Procedure-related factors, including left ventricular lead position and a high percentage of biventricular pacing, are critical for success. A multimodal approach that combines clinical, electrocardiographic, echocardiographic and imaging data, supported in the future by machine learning, appears most promising. FAU - Potapowicz-Krysztofiak, Magdalena AU - Potapowicz-Krysztofiak M AUID- ORCID: 0009-0006-1761-8861 AD - Department of Cardiology and Internal Diseases, Military Institute of Medicine-National Research Institute, 04-141 Warsaw, Poland. FAU - Maciorowska, Malgorzata AU - Maciorowska M AUID- ORCID: 0000-0001-5242-7522 AD - Department of Cardiology and Internal Diseases, Military Institute of Medicine-National Research Institute, 04-141 Warsaw, Poland. FAU - Uzieblo-Zyczkowska, Beata AU - Uzieblo-Zyczkowska B AUID- ORCID: 0000-0001-8997-6568 AD - Department of Cardiology and Internal Diseases, Military Institute of Medicine-National Research Institute, 04-141 Warsaw, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261003 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701934 OTO - NOTNLM OT - cardiac resynchronization therapy OT - heart failure OT - left bundle branch block OT - mechanical dyssynchrony OT - predictors OT - responder COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/03 CRDT- 2026/10/27 10:35 PHST- 2026/09/13 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - biomedicines14102239 [pii] AID - biomedicines-14-02239 [pii] AID - 10.3390/biomedicines14102239 [doi] PST - epublish SO - Biomedicines. 2026 Oct 3;14(10):2239. doi: 10.3390/biomedicines14102239. PMID- 42940043 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 3 TI - Gene-Editing in Respiratory Disease: From Pre-Clinical Human Models to Future Therapeutics. LID - 10.3390/biomedicines14102237 [doi] LID - 2237 AB - Chronic lung diseases arise from complex interactions between environmental exposures and host genetic factors. However, these interactions are not fully understood, and despite decades of study, effective curative therapies remain unavailable for many patients. Recent breakthroughs in therapeutic gene-editing have become a reality for some inherited blood diseases and are a promising approach for monogenic lung diseases. Early-phase clinical studies are now ongoing for AATD, primarily through liver-directed editing of hepatocytes to restore circulating functional AAT and for COVID-19 using gene-edited immune cell approaches. However, direct in vivo gene-editing of pulmonary epithelial cells remains largely at the preclinical stage. Expanding this therapeutic pipeline requires overcoming the unique biological barriers of the lung and developing human-specific models that faithfully recapitulate human disease mechanisms. Advances in gene-editing technologies, including CRISPR-Cas9 systems, base and prime editors, and emerging compact nucleases, have increased the feasibility of precise genomic correction. However, safe and efficient delivery directly to the lung remains a major unmet challenge due to mucus, surfactant, epithelial tight junctions, and innate immune surveillance. Delivery systems such as engineered AAVs, lentiviral vectors, selective organ targeting lipid nanoparticles and aerosolizable formulations like nebulised or dry powder nanoparticles are beginning to address these obstacles. In parallel, human preclinical models have evolved from traditional cell lines to more sophisticated platforms such as iPSC-derived airway and alveolar cells, organoids, precision-cut lung slices, air-liquid interface cultures, organ-on-a-chip devices, and patient-derived primary cells. These models enable mechanistic interrogation, variant-specific functional assessment, and preclinical evaluation of gene-editing strategies with increased physiological relevance. In this review, we detail current and emerging gene-editing tools, delivery strategies, and human-relevant disease models. We highlight key translational barriers and discuss how advanced human models combined with improved delivery technologies can accelerate the development of safe and effective gene-editing therapeutics for chronic respiratory disease. FAU - Duffy, Amy AU - Duffy A AD - Department of Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin 9, D09 YD60 Dublin, Ireland. AD - Tissue Engineering Research Group, Royal College of Surgeons in Ireland, Dublin 2, D02 YN77 Dublin, Ireland. FAU - Schweikert, Anja AU - Schweikert A AD - Department of Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin 9, D09 YD60 Dublin, Ireland. AD - Tissue Engineering Research Group, Royal College of Surgeons in Ireland, Dublin 2, D02 YN77 Dublin, Ireland. FAU - Tan, Sze Ying AU - Tan SY AUID- ORCID: 0000-0003-0319-7112 AD - Department of Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin 9, D09 YD60 Dublin, Ireland. FAU - Murphy, Cormac AU - Murphy C AUID- ORCID: 0009-0005-6529-4040 AD - Department of Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin 9, D09 YD60 Dublin, Ireland. AD - Tissue Engineering Research Group, Royal College of Surgeons in Ireland, Dublin 2, D02 YN77 Dublin, Ireland. FAU - Dowling, Catriona M AU - Dowling CM AD - Department of Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin 9, D09 YD60 Dublin, Ireland. FAU - Hurley, Killian AU - Hurley K AUID- ORCID: 0000-0003-4755-5559 AD - Department of Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin 9, D09 YD60 Dublin, Ireland. AD - Tissue Engineering Research Group, Royal College of Surgeons in Ireland, Dublin 2, D02 YN77 Dublin, Ireland. FAU - Ozaki, Mari AU - Ozaki M AUID- ORCID: 0000-0003-0649-7882 AD - Department of Medicine, Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin 9, D09 YD60 Dublin, Ireland. AD - Tissue Engineering Research Group, Royal College of Surgeons in Ireland, Dublin 2, D02 YN77 Dublin, Ireland. LA - eng PT - Journal Article PT - Review DEP - 20261003 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701671 OTO - NOTNLM OT - CRISPR OT - disease modelling OT - gene-editing OT - gene-therapies OT - genetic lung disease OT - iPSCs COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/03 CRDT- 2026/10/27 10:35 PHST- 2026/08/17 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - biomedicines14102237 [pii] AID - biomedicines-14-02237 [pii] AID - 10.3390/biomedicines14102237 [doi] PST - epublish SO - Biomedicines. 2026 Oct 3;14(10):2237. doi: 10.3390/biomedicines14102237. PMID- 42940042 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 2 TI - Total Phenylethanoid Glycosides from Cistanche tubulosa Alleviate 5-Fluorouracil-Induced Fatigue-Like Behavior and Skeletal Muscle Injury in Mice. LID - 10.3390/biomedicines14102236 [doi] LID - 2236 AB - Objectives: This study investigated the effects of total phenylethanoid glycosides from Cistanche tubulosa (CPhGs) on 5-fluorouracil (5-FU)-induced fatigue-like behavior and tissue injury in mice and examined associated molecular changes. Methods: Public transcriptomic datasets (GSE80473, GSE121972, and GSE77745) were analyzed to provide exploratory pathway-level context for hypothesis-driven markers associated with chemotherapy- or cachexia-related skeletal muscle injury. Forty tumor-free BALB/c mice were randomly assigned to a normal control group or treated with 5-FU (20 mg/kg, intraperitoneally) for five consecutive days. The 5-FU-treated mice were subsequently divided into model, low-dose-CPhG (250 mg/kg), and high-dose-CPhG (500 mg/kg) groups and treated orally for 14 days. Exercise endurance, forelimb grip strength, serum markers of tissue injury and inflammation, and histopathological changes in skeletal muscle, epididymal adipose tissue, and liver were evaluated. Proteins involved in Akt/FoxO3a signaling, muscle protein degradation, and mitochondrial metabolism were measured in skeletal muscle. Molecular docking was used to assess the predicted interactions of echinacoside and acteoside with selected proteins. Results: Exploratory transcriptomic analysis suggested alterations in muscle contraction, fatty acid oxidation and energy metabolism, with external cachexia data providing context for FoxO/ubiquitin-proteasome signaling and inflammation in skeletal muscle injury. Compared with control mice, 5-FU-treated mice showed body weight loss, reduced swimming endurance, decreased forelimb grip strength, elevated serum CK, CK-MB, LDH, LDH1, TNF-alpha, and IL-6 levels, and pathological changes in skeletal muscle, adipose tissue, and liver. CPhGs attenuated body weight loss, prolonged swimming time, improved forelimb grip strength, reduced serum markers of tissue injury and inflammation, and improved tissue morphology. CPhGs were also associated with lower skeletal muscle MuRF1 and Atrogin-1 expression and higher p-Akt/Akt and p-FoxO3a/FoxO3a ratios and PGC-1alpha expression. These effects were generally more pronounced at the higher dose. Docking analysis generated exploratory poses of echinacoside and acteoside with AKT1, MuRF1, and IL-6; the docking scores do not establish binding affinity or target engagement. Conclusions: CPhGs alleviated 5-FU-induced fatigue-like behavior and associated skeletal muscle, adipose tissue, and hepatic abnormalities in mice. These effects were associated with regulation of Akt/FoxO3a signaling, lower expression of proteolysis-associated proteins, higher PGC-1alpha expression, and reduced systemic inflammation. The in vivo findings concern the complete CPhG preparation; the contributions and direct molecular targets of echinacoside and acteoside require experimental confirmation. FAU - Chen, Jinjin AU - Chen J AUID- ORCID: 0009-0008-0983-7663 AD - School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China. FAU - Yang, Jianhua AU - Yang J AD - School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China. FAU - Cao, Yexia AU - Cao Y AD - School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China. FAU - Wen, Limei AU - Wen L AD - School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China. FAU - Su, Xufeng AU - Su X AD - School of Public Health, Xinjiang Medical University, Urumqi 830011, China. FAU - Yan, Jiayuan AU - Yan J AD - School of Public Health, Xinjiang Medical University, Urumqi 830011, China. FAU - Hou, Qiang AU - Hou Q AD - School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China. FAU - Zhang, Shilei AU - Zhang S AD - School of Public Health, Xinjiang Medical University, Urumqi 830011, China. FAU - Hu, Junping AU - Hu J AD - School of Pharmacy, Xinjiang Medical University, Urumqi 830017, China. LA - eng GR - 81860735/National Natural Science Foundation of China/ GR - 0103010211/the 14-th Five-Vear PlanDistinctive Program of Public Health and Preventive Medicine inHigher Education Institutions of Xinjiang Uygur Autonomous Region,and Special Funds for Talents of Xinjiang Medical University/ GR - 82160772/National Natural Science Foundation of China/ GR - 2021D01D11/People's Government of Xinjiang Uygur Autonomous Region/ GR - 2022D01D14/People's Government of Xinjiang Uygur Autonomous Region/ GR - XJDX1713/the Key Laboratory of Natural Drug Active Components and Drug Release Technology in Xinjiang/ GR - 2024TSYCLJ0048/the Science and Technology Innovation Leadership Talent Program/ GR - 82660941/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261002 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701381 OTO - NOTNLM OT - Cistanche tubulosa OT - chemotherapy-induced fatigue-like behavior OT - phenylethanoid glycosides OT - skeletal muscle injury COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/02 CRDT- 2026/10/27 10:35 PHST- 2026/09/09 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/02 00:00 [pmc-release] AID - biomedicines14102236 [pii] AID - biomedicines-14-02236 [pii] AID - 10.3390/biomedicines14102236 [doi] PST - epublish SO - Biomedicines. 2026 Oct 2;14(10):2236. doi: 10.3390/biomedicines14102236. PMID- 42940038 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 2 TI - Cytokine and Vasopressor Trajectories During Continuous Renal Replacement Therapy with oXiris, CytoSorb, or Standard Haemofilters in Sepsis-Associated Acute Kidney Injury: An Exploratory Retrospective Cohort Study. LID - 10.3390/biomedicines14102230 [doi] LID - 2230 AB - Background/Objectives: This exploratory study described interleukin-6 (IL-6) and vasopressor-dependency trajectories during continuous renal replacement therapy (CRRT) with oXiris, CytoSorb, or standard haemofilters in sepsis-associated acute kidney injury, and their lagged and prognostic associations. Methods: This retrospective single-centre cohort included 48 adults receiving standard CRRT (n = 22), oXiris (n = 21), or CytoSorb (n = 5; analysed descriptively), assessed at baseline and 12, 24, and 48 h; devices were clinician-selected, implying confounding by indication. The vasopressor dependency index (VDI) was vasoactive-inotropic score/mean arterial pressure. Results: Twenty-eight-day survival was 13.6%, 33.3%, and 40.0%, respectively (p = 0.240). The unadjusted 24 h VDI decline was largest with CytoSorb (p = 0.018), but this group had much higher baseline IL-6 and VDI, and the contrast was attenuated and non-significant after baseline adjustment. No lagged association was detectable between IL-6 and subsequent VDI (beta = 0.04, 95% confidence interval (CI) -0.04 to 0.13) or VDI and subsequent IL-6 (beta = -0.05, 95% CI -0.20 to 0.09). In a 24 h landmark analysis, favourable haemodynamic status was associated with survival (odds ratio 7.57, 95% CI 1.75-32.69) without improving optimism-corrected discrimination beyond baseline severity and age (area under the curve 0.882). Conclusions: These hypothesis-generating findings do not establish a benefit of any device; early haemodynamic response criteria warrant prospective evaluation. FAU - Porosnicu, Tamara Mirela AU - Porosnicu TM AUID- ORCID: 0009-0001-8302-8003 AD - Department of Anaesthesia and Intensive Care, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041 Timisoara, Romania. FAU - Gindac, Ciprian AU - Gindac C AD - Department of Anaesthesia and Intensive Care, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041 Timisoara, Romania. FAU - Elagez, Ahmed AU - Elagez A AD - Mercyhealth Family Medicine Residency Program-Rockford, Mercyhealth Alpine, 7702 North Alpine Road, Loves Park, IL 61111, USA. FAU - Papurica, Marius AU - Papurica M AUID- ORCID: 0000-0002-4065-3650 AD - Department of Anaesthesia and Intensive Care, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041 Timisoara, Romania. FAU - Pescariu, Alexandru Silvius AU - Pescariu AS AUID- ORCID: 0000-0002-1697-311X AD - Department VI-Cardiology, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041 Timisoara, Romania. FAU - Bratosin, Felix AU - Bratosin F AUID- ORCID: 0000-0003-4711-4315 AD - Department of Infectious Diseases, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041 Timisoara, Romania. FAU - Hogea, Elena AU - Hogea E AUID- ORCID: 0009-0008-8805-2046 AD - Department XIV, Discipline of Microbiology, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041 Timisoara, Romania. FAU - Sandesc, Dorel AU - Sandesc D AD - Department of Anaesthesia and Intensive Care, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041 Timisoara, Romania. FAU - Bedreag, Ovidiu AU - Bedreag O AUID- ORCID: 0000-0002-3432-9168 AD - Department of Anaesthesia and Intensive Care, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square 2, 300041 Timisoara, Romania. LA - eng PT - Journal Article DEP - 20261002 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701235 OTO - NOTNLM OT - continuous renal replacement therapy OT - hemoadsorption OT - interleukin-6 OT - sepsis OT - vasoconstrictor agents COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/02 CRDT- 2026/10/27 10:35 PHST- 2026/09/15 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/02 00:00 [pmc-release] AID - biomedicines14102230 [pii] AID - biomedicines-14-02230 [pii] AID - 10.3390/biomedicines14102230 [doi] PST - epublish SO - Biomedicines. 2026 Oct 2;14(10):2230. doi: 10.3390/biomedicines14102230. PMID- 42940036 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 2 TI - Alternative Splicing of Homologous Recombination Genes Is Associated with Homologous Recombination Deficiency and Immunoregulatory Phenotypes in Breast Cancer. LID - 10.3390/biomedicines14102231 [doi] LID - 2231 AB - Background/Objectives: Homologous recombination deficiency (HRD) is an important feature of breast cancer, but the relationship between alternative splicing (AS) of homologous recombination (HR) genes and HRD remains poorly characterized. The study investigated whether coordinated HR-gene AS is associated with genomic HRD and immune phenotypes. Methods: Using The Cancer Genome Atlas Breast Invasive Carcinoma (TCGA-BRCA) cohort, we developed a normal-referenced, module-balanced HR splicing disruption score (HRSD) from 186 AS events across 49 HR genes. Associations with HRD were evaluated across molecularly restricted subsets and by multivariable analysis. HRD-associated splicing features were assessed across six immune phenotypes. A prioritized RAD54B alternative termination event was characterized further and independently evaluated in the Sweden Cancerome Analysis Network-Breast (SCAN-B) cohort. Results: HRSD was positively associated with genomic HRD (rho = 0.338, p = 1.64 x 10(-21)) and remained significant after restriction for HRD-associated molecular features and exclusion of basal tumors. In multivariable models, each 1-SD increase in HRSD was independently associated with higher HRD scores (beta = 3.17, p = 7.08 x 10(-7)) and HRD-high status (OR = 1.6, p = 0.001). HRD-associated splicing converged with immunoregulatory phenotypes, particularly regulatory T-cells and checkpoint activity. RAD54B alternative termination (RAD54B|AT|84553) was higher in HRD-high tumors (p = 3.42 x 10(-14)) and remained independently associated with immunoregulatory phenotypes after adjustment. SCAN-B analysis validated the terminal-exon structure and reproduced subtype-specific termination patterns. Conclusions: HR-associated AS represents a transcript-level dimension of HRD biology in breast cancer. RAD54B alternative termination may provide a candidate link between HRD-associated splicing and immunoregulatory phenotypes, warranting further functional investigation. FAU - Basudan, Ahmed M AU - Basudan AM AUID- ORCID: 0000-0002-6689-7004 AD - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 12372, Saudi Arabia. FAU - Alshuweishi, Yazeed AU - Alshuweishi Y AUID- ORCID: 0000-0002-9476-8197 AD - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 12372, Saudi Arabia. FAU - AlSudais, Hamood AU - AlSudais H AUID- ORCID: 0000-0001-8228-1392 AD - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 12372, Saudi Arabia. FAU - Alfhili, Mohammad A AU - Alfhili MA AUID- ORCID: 0000-0002-3095-9154 AD - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 12372, Saudi Arabia. LA - eng GR - ORF-2026-1553/Ongoing Research Funding program, (ORF-2026-1553), King Saud University, Riyadh, Saudi Arabia/ PT - Journal Article DEP - 20261002 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701930 OTO - NOTNLM OT - RAD54B OT - alternative splicing OT - breast cancer OT - homologous recombination deficiency OT - immunoregulatory phenotypes COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/02 CRDT- 2026/10/27 10:35 PHST- 2026/09/12 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/02 00:00 [pmc-release] AID - biomedicines14102231 [pii] AID - biomedicines-14-02231 [pii] AID - 10.3390/biomedicines14102231 [doi] PST - epublish SO - Biomedicines. 2026 Oct 2;14(10):2231. doi: 10.3390/biomedicines14102231. PMID- 42940034 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 1 TI - EASIX for Long-Term Prognostication in Hospitalized Heart Failure with Preserved Ejection Fraction: Incremental Value Beyond Clinical Risk Markers. LID - 10.3390/biomedicines14102228 [doi] LID - 2228 AB - Background/Objectives: Heart failure with preserved ejection fraction (HFpEF) is a heterogeneous multisystem syndrome in which conventional cardiac measures may not fully capture systemic vulnerability. The Endothelial Activation and Stress Index (EASIX) is prognostic in other cardiovascular populations, but evidence in HFpEF remains limited. We evaluated the association of EASIX with long-term mortality and compared its prognostic value with the Prognostic Nutritional Index (PNI) and Controlling Nutritional Status (CONUT) score. Methods: This retrospective single-center study included 79 adults hospitalized with HFpEF between 2019 and 2025. EASIX, PNI, and CONUT were calculated from admission laboratory values. The endpoint was all-cause mortality. EASIX was modeled using restricted cubic splines. Incremental value beyond age, serum sodium, and B-type natriuretic peptide was assessed using likelihood-ratio tests. Harrell's C-index was internally validated using 1000 bootstrap resamples; conventional and time-dependent receiver operating characteristic analyses were also performed. Results: During a median follow-up of 27.6 months, 45 patients (57.0%) died. EASIX was nonlinearly associated with mortality (overall and nonlinearity p < 0.001) and improved clinical model fit (Deltachi(2) = 24.36; p < 0.001). PNI improved the clinical model without EASIX (Deltachi(2) = 8.74; p = 0.003), but its contribution after EASIX was attenuated (Deltachi(2) = 3.54; p = 0.060); CONUT did not improve model fit (p = 0.859). Optimism-corrected C-indices were 0.728 for clinical + EASIX and 0.732 after adding PNI. Conventional AUCs were 0.753, 0.782, and 0.661 for EASIX, PNI, and CONUT, respectively. Conclusions: In this selected hospitalized HFpEF cohort, EASIX provided incremental prognostic information beyond clinical markers. These exploratory findings require confirmation in larger prospective cohorts with external validation. FAU - Alak, Cetin AU - Alak C AUID- ORCID: 0000-0003-1875-2078 AD - Department of Cardiology, Faculty of Medicine, Bursa Uludag University, 16059 Bursa, Turkiye. FAU - Turhan, Kaan AU - Turhan K AD - Department of Cardiology, Faculty of Medicine, Bursa Uludag University, 16059 Bursa, Turkiye. FAU - Hunutlu, Fazil Cagri AU - Hunutlu FC AUID- ORCID: 0000-0002-4991-9830 AD - Division of Hematology, Department of Internal Medicine, Bursa Sehir Training and Research Hospital, University of Health Sciences, 16009 Bursa, Turkiye. FAU - Kumral, Zeynep AU - Kumral Z AUID- ORCID: 0000-0003-2422-9363 AD - Department of Cardiology, Unye State Hospital, 52300 Ordu, Turkiye. FAU - Senturk, Tunay AU - Senturk T AD - Department of Cardiology, Faculty of Medicine, Bursa Uludag University, 16059 Bursa, Turkiye. LA - eng PT - Journal Article DEP - 20261001 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701619 OTO - NOTNLM OT - CONUT score OT - all-cause mortality OT - endothelial activation and stress index OT - heart failure with preserved ejection fraction OT - prognostic biomarkers OT - prognostic nutritional index OT - risk stratification COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:35 PHST- 2026/08/18 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - biomedicines14102228 [pii] AID - biomedicines-14-02228 [pii] AID - 10.3390/biomedicines14102228 [doi] PST - epublish SO - Biomedicines. 2026 Oct 1;14(10):2228. doi: 10.3390/biomedicines14102228. PMID- 42940027 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 1 TI - Heart Rate Variability in Cardiometabolic Disease: From Molecular Mechanisms to Personalized Exercise Prescription. LID - 10.3390/biomedicines14102221 [doi] LID - 2221 AB - Cardiometabolic diseases are characterized by interconnected disturbances in metabolic, inflammatory, mitochondrial, vascular, endocrine, and autonomic regulation, highlighting the need for integrative biomarkers capable of capturing systemic physiological dysfunction. Heart rate variability (HRV) provides a non-invasive and dynamic measure of cardiovascular autonomic regulation and may reflect the cumulative physiological consequences of these interacting alterations. This narrative review synthesizes current evidence on the role of HRV in cardiometabolic disease, with particular emphasis on the molecular and physiological mechanisms linking autonomic dysfunction with metabolic, inflammatory, mitochondrial, and vascular disturbances, as well as its potential application in individualized exercise prescription. Across obesity, metabolic syndrome, insulin resistance, type 2 diabetes, hypertension, and cardiovascular disease, reduced HRV is consistently associated with impaired autonomic adaptability and increased cardiometabolic risk. However, HRV should not be interpreted as a direct measure of sympathovagal balance or as a molecular biomarker; rather, it represents an integrative physiological signal influenced by multiple biological, behavioral, and environmental factors. Exercise may modulate autonomic regulation while simultaneously influencing metabolic signaling, inflammation, mitochondrial function, vascular health, and recovery, and accumulating evidence indicates that improvements in HRV may accompany favorable adaptations across diverse cardiometabolic populations. Accordingly, repeated HRV assessment may complement clinical, metabolic, functional, and body-composition measures to characterize physiological adaptation and support individualized adjustments in exercise prescription. Overall, HRV represents a promising integrative marker of cardiometabolic health and physiological resilience. Nevertheless, methodological heterogeneity, physiological confounding, and limited causal evidence currently constrain its clinical implementation. Standardized longitudinal and mechanistic studies are warranted to establish clinically meaningful HRV phenotypes, determine their capacity to predict individual exercise responsiveness, and clarify whether HRV-guided interventions can ultimately improve cardiometabolic outcomes. FAU - Fuentes-Barria, Hector AU - Fuentes-Barria H AUID- ORCID: 0000-0003-0774-0848 AD - Centro de Investigacion en Medicina de Altura (CEIMA), Universidad Arturo Prat, Iquique 1110939, Chile. FAU - Aguilera-Eguia, Raul AU - Aguilera-Eguia R AUID- ORCID: 0000-0002-4123-4255 AD - Departamento de Salud Publica, Facultad de Medicina, Universidad Catolica de la Santisima Concepcion, Concepcion 3349001, Chile. FAU - Alarcon-Rivera, Miguel AU - Alarcon-Rivera M AUID- ORCID: 0000-0001-8341-5567 AD - Escuela de Ciencias del Deporte y Actividad Fisica, Facultad de Salud, Universidad Santo Tomas, Talca 3460000, Chile. FAU - Flores-Fernandez, Cherie AU - Flores-Fernandez C AUID- ORCID: 0000-0001-5294-7157 AD - Departamento de Gestion de la Informacion, Universidad Tecnologica Metropolitana, Santiago 7550000, Chile. FAU - Perez, Diego Ignacio Valenzuela AU - Perez DIV AUID- ORCID: 0000-0002-9884-1187 AD - Facultad de Salud y Ciencias Sociales, Universidad de las Americas, Santiago 8370003, Chile. FAU - Angarita-Davila, Lisse AU - Angarita-Davila L AUID- ORCID: 0000-0001-7860-5112 AD - Escuela de Nutricion y Dietetica, Facultad de Medicina, Universidad Andres Bello, Concepcion 3349001, Chile. FAU - Roco-Videla, Angel AU - Roco-Videla A AUID- ORCID: 0000-0001-8850-1018 AD - Direccion de Desarrollo y Postgrado, Universidad Autonoma de Chile, Santiago 7500912, Chile. LA - eng PT - Journal Article PT - Review DEP - 20261001 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701700 OTO - NOTNLM OT - autonomic nervous system OT - cardiovascular diseases OT - exercise therapy OT - heart rate determination COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:35 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - biomedicines14102221 [pii] AID - biomedicines-14-02221 [pii] AID - 10.3390/biomedicines14102221 [doi] PST - epublish SO - Biomedicines. 2026 Oct 1;14(10):2221. doi: 10.3390/biomedicines14102221. PMID- 42940025 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Oct 1 TI - Urinary and Sexual Function Outcomes After Ultrasound-Guided High-Intensity Focused Ultrasound for Uterine Fibroids: Associations of Lower Urinary Tract Symptoms with Fibroid Location and Uterine Morphology. LID - 10.3390/biomedicines14102219 [doi] LID - 2219 AB - Background/Objectives: Uterine fibroids may contribute to lower urinary tract symptoms (LUTS) through pelvic mass effects, but urinary and sexual outcomes after ultrasound-guided high-intensity focused ultrasound (USgHIFU) and their anatomical correlates remain unclear. We evaluated 6-month urinary and sexual outcomes, described urinary changes by dominant fibroid location, and explored associations between uterine morphology and baseline urinary symptom burden. Methods: This retrospective study included 75 women with paired baseline and 6-month urinary assessments (anterior, n = 45; non-anterior, n = 30). Measures included the Overactive Bladder Symptom Score (OABSS), Urogenital Distress Inventory-6 (UDI-6), Incontinence Impact Questionnaire-7 (IIQ-7), International Consultation on Incontinence Questionnaire-Urinary Incontinence Short Form (ICIQ-UI SF), and Female Sexual Function Index (FSFI; n = 63); pretreatment magnetic resonance imaging (MRI) characterized uterine anatomy. Results: At 6 months, OABSS decreased from 3.51 +/- 3.07 to 2.57 +/- 2.29 (p = 0.005); UDI-6, IIQ-7, and ICIQ-UI SF also improved (all p /= 3 prevalence declined from 53.3% to 40.0% (p = 0.002). Mean urinary symptom scores decreased in both location groups. The larger longitudinal-diameter subgroup had higher baseline OABSS (4.20 vs. 2.71; p = 0.034) and OABSS >/= 3 prevalence (70.0% vs. 34.3%; p = 0.003), whereas the corresponding comparisons for uterine volume and anteroposterior diameter were not significantly associated. The mean FSFI total score increased from 21.6 +/- 5.8 to 26.5 +/- 6.6 (summary-derived conservative p-value bound, p < 0.005). Conclusions: USgHIFU was associated with improved urinary symptoms, urinary symptom-related quality of life, and sexual function. The exploratory association between the longitudinal-diameter groups and baseline overactive bladder symptom burden warrants confirmation using continuous measurements and adjusted analyses. FAU - Long, Cheng-Yu AU - Long CY AD - Department of Obstetrics and Gynecology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, Taiwan. AD - Department of Obstetrics and Gynecology, College of Medicine, Kaohsiung Medical University, Kaohsiung 807378, Taiwan. FAU - Jeng, Cherng-Jye AU - Jeng CJ AD - Department of Obstetrics and Gynecology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, Taiwan. AD - Department of Obstetrics and Gynecology, College of Medicine, Kaohsiung Medical University, Kaohsiung 807378, Taiwan. AD - Department of Gynecology, Leo Minimally Invasive International Hospital, New Taipei City 244019, Taiwan. FAU - Ke, Liang-Ying AU - Ke LY AD - Department of Obstetrics and Gynecology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, Taiwan. AD - Department of Obstetrics and Gynecology, College of Medicine, Kaohsiung Medical University, Kaohsiung 807378, Taiwan. AD - Department of Obstetrics and Gynecology, Kaohsiung Municipal Siaogang Hospital, Kaohsiung Medical University, Kaohsiung 812, Taiwan. LA - eng PT - Journal Article DEP - 20261001 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701314 OTO - NOTNLM OT - high-intensity focused ultrasound OT - lower urinary tract symptoms OT - overactive bladder OT - uterine fibroids OT - uterine morphology COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:35 PHST- 2026/09/13 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - biomedicines14102219 [pii] AID - biomedicines-14-02219 [pii] AID - 10.3390/biomedicines14102219 [doi] PST - epublish SO - Biomedicines. 2026 Oct 1;14(10):2219. doi: 10.3390/biomedicines14102219. PMID- 42940024 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 30 TI - Integrated Analysis of Serotonin-Related Biomarkers in Thoracic Aortic Aneurysm Based on Machine Learning and Single-Cell Sequencing Data. LID - 10.3390/biomedicines14102218 [doi] LID - 2218 AB - Background: Serotonin, as an important neurotransmitter and signaling molecule, can affect thoracic aortic aneurysm (TAA) by regulating vascular cell proliferation, angiogenesis, and immune status. This research aimed to conduct a comprehensive analysis to pinpoint serotonin-related biomarkers in TAA and to elucidate their potential molecular mechanisms. Methods: Biomarkers were selected and validated through differential expression analysis, weighted gene co-expression network analysis (WGCNA), machine learning algorithms, and expression verification. Moreover, the constructed nomogram, enrichment analysis, immune infiltration analysis, and compound prediction validated the regulatory roles of the biomarkers in TAA. The single-cell dataset was analyzed to determine key cells and perform pseudo-time analysis. Simulated knockout of biomarkers was performed to determine their underlying functions and related pathways. Finally, reverse transcription quantitative PCR (RT-qPCR) was employed to confirm the expression levels of the biomarkers. Results: Overall, two biomarkers (CD300A and CLU) were determined to be associated with serotonin in TAA. The nomogram showed good performance in predicting TAA risk. These biomarkers were significantly enriched in the hematopoietic cell lineage and natural killer (NK) cell mediated cytotoxicity. Biomarker expression was significantly correlated with the infiltration proportions of M2 macrophages and neutrophils. The biomarkers were stably bound to tetrachlorodibenzodioxin and bisphenol A. Moreover, pseudo-time analysis demonstrated a notable correlation between biomarker expression and the differentiation status of monocytes/macrophages/dendritic cells (MonoMac/DC). Genes perturbed by knockout were enriched in the pyrimidine metabolism and RIG-I-like receptor signaling pathway. Finally, RT-qPCR validated that CLU was downregulated and CD300A was upregulated in the TAA group. Conclusions: Biomarkers (CD300A and CLU) associated with serotonin in TAA were identified, with their expression trends validated by RT-qPCR. This work provides novel bioinformatic insights, and direct functional experiments are required to support future therapeutic development for TAA. FAU - Zhu, Shichao AU - Zhu S AUID- ORCID: 0000-0001-5057-9434 AD - Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China. FAU - Abudupataer, Mieradilijiang AU - Abudupataer M AD - Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China. FAU - Li, Hua AU - Li H AD - Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China. FAU - Zuo, Zheng AU - Zuo Z AD - Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China. FAU - Zhai, Junyu AU - Zhai J AD - Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China. FAU - Yan, Tao AU - Yan T AD - Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China. FAU - Zhang, Hongqiang AU - Zhang H AD - Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China. FAU - Lai, Hao AU - Lai H AD - Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China. FAU - Wang, Chunsheng AU - Wang C AD - Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China. FAU - Zhu, Kai AU - Zhu K AD - Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China. FAU - Huang, Ben AU - Huang B AUID- ORCID: 0000-0002-0015-6721 AD - Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China. FAU - Chen, Nan AU - Chen N AD - Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China. LA - eng GR - 82200525/National Natural Science Foundation of China/ GR - 82300543/National Natural Science Foundation of China/ PT - Journal Article DEP - 20260930 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701274 OTO - NOTNLM OT - biomarker OT - machine learning OT - serotonin OT - single-cell RNA sequencing OT - thoracic aortic aneurysm COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:35 PHST- 2026/08/01 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - biomedicines14102218 [pii] AID - biomedicines-14-02218 [pii] AID - 10.3390/biomedicines14102218 [doi] PST - epublish SO - Biomedicines. 2026 Sep 30;14(10):2218. doi: 10.3390/biomedicines14102218. PMID- 42940015 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 29 TI - Machine Learning Based on Routine Hematological Parameters and Derived Inflammatory Indices for the Diagnosis of Schizophrenia. LID - 10.3390/biomedicines14102209 [doi] LID - 2209 AB - Background: Schizophrenia is frequently underdiagnosed or diagnosed late due to the lack of objective diagnostic markers. This study aimed to develop and validate a machine learning model using routine hematological parameters for the auxiliary diagnosis of schizophrenia. Methods: A total of 150 first-episode drug-naive schizophrenia patients and 150 healthy controls were enrolled. Study parameters included routine hematological parameters and its derived inflammatory markers. Feature selection was performed using Elastic Net regression, followed by the construction of an L2-regularized logistic regression model. Model discriminative performance, calibration, and clinical utility were assessed through internal validation, area under the receiver operating characteristic curve (AUC), calibration curves, and decision curve analysis. An independent cohort of 50 schizophrenia patients and 50 patients with major depressive disorder (MDD) was used for exploratory differential diagnostic evaluation. Results: The final model incorporated 12 features: neutrophil count (NEUT), eosinophil count (EO), mean platelet volume (MPV), hematocrit (HCT), hemoglobin (HGB), neutrophil-to-lymphocyte ratio (NLR), lymphocyte-to-monocyte ratio (LMR), systemic immune-inflammation index (SII), systemic inflammation response index (SIRI), neutrophil-to-platelet ratio (NPR), along with age and sex. The model achieved an AUC of 0.859 (95% CI: 0.780-0.937) on the test set, with an accuracy of 0.767, sensitivity of 0.711, and specificity of 0.822. Calibration curves confirmed good calibration, and decision curve analysis further verified its clinical utility. In the exploratory differential diagnostic analysis, the model showed limited performance in distinguishing schizophrenia from MDD. Conclusions: The model based on routine hematological parameters and L2-regularized logistic regression can effectively differentiate first-episode drug-naive schizophrenia patients from healthy controls, providing a low-cost and easily accessible auxiliary diagnostic tool for clinical practice. FAU - Fu, Xiaomei AU - Fu X AUID- ORCID: 0000-0002-8767-6809 AD - Department of Medical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China. FAU - Jin, Weifeng AU - Jin W AD - Department of Medical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China. FAU - Li, Dan AU - Li D AD - Department of Medical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China. FAU - Li, Zhenhua AU - Li Z AUID- ORCID: 0000-0002-1075-0214 AD - Department of Medical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China. FAU - Chen, Qing AU - Chen Q AD - Department of Medical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China. FAU - Chen, Shuzi AU - Chen S AD - Department of Medical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China. FAU - Ma, Peijun AU - Ma P AUID- ORCID: 0000-0001-7979-3338 AD - Department of Medical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China. FAU - Zhu, Mengyuan AU - Zhu M AD - Department of Medical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China. FAU - Wang, Mengxia AU - Wang M AD - Department of Medical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China. FAU - Qu, Caiwei AU - Qu C AD - Department of Medical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China. FAU - Pan, Ruoxuan AU - Pan R AD - Department of Medical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China. FAU - Chai, Zhiyun AU - Chai Z AD - Department of Medical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China. FAU - Lin, Ping AU - Lin P AD - Department of Medical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China. LA - eng GR - 2024ZZ1032/Shanghai Municipal Health Commission Medical New Technology Research and Transformation Seed Program, Establishment and Application Transformation of Key Technology System for Precise Diagnosis of Cognitive Impairment in the Elderly/ PT - Journal Article DEP - 20260929 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701796 OTO - NOTNLM OT - machine learning OT - major depressive disorder OT - routine hematological parameters OT - schizophrenia COIS- The authors declare no conflicts of interest. The sponsors had no role in the design, execution, interpretation, or writing of the study. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/29 CRDT- 2026/10/27 10:35 PHST- 2026/08/05 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/29 00:00 [pmc-release] AID - biomedicines14102209 [pii] AID - biomedicines-14-02209 [pii] AID - 10.3390/biomedicines14102209 [doi] PST - epublish SO - Biomedicines. 2026 Sep 29;14(10):2209. doi: 10.3390/biomedicines14102209. PMID- 42940010 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 29 TI - Regenerative Strategies for Osteoporotic Craniomaxillofacial Bone Defects: Evidence, Translational Gaps, and Future Directions. LID - 10.3390/biomedicines14102204 [doi] LID - 2204 AB - BACKGROUND: Osteoporosis alters bone remodeling, osteogenic competence, redox and inflammatory homeostasis, and vascular support. These abnormalities may compromise healing of craniomaxillofacial defects, but evidence for regenerative interventions is distributed across mechanistic studies, non-craniofacial osteoporotic models, craniofacial models without osteoporosis, and a smaller body of direct osteoporotic craniofacial evidence. METHODS: A literature search was conducted using PubMed and Web of Science. Search terms combined osteoporosis-related terms with bone regeneration/defect healing, craniomaxillofacial anatomy, and intervention-specific terms. No strict publication-date restriction was applied, and studies were selected according to their relevance to the predefined scope. Evidence was interpreted using two complementary dimensions: directness to osteoporotic craniomaxillofacial regeneration and translational maturity. RESULTS: Direct osteoporotic craniofacial preclinical evidence is available for selected interventions, including melatonin and injectable platelet-rich fibrin, whereas vitamin D, teriparatide, bone morphogenetic proteins, mesenchymal stem cell-based therapies, exosomes, hydrogels, nanomaterials, and 3D-printed constructs are supported by different combinations of indirect osteoporotic, non-osteoporotic craniofacial, mechanistic, and clinical evidence. Teriparatide and recombinant human bone morphogenetic protein-2 have comparatively mature clinical pathways, but their clinical evidence is not synonymous with efficacy in osteoporotic craniomaxillofacial defects. Emerging biomaterials can address delivery, retention, oxidative stress, inflammation, angiogenesis, and osteogenic deficits simultaneously, but remain predominantly preclinical. CONCLUSIONS: Direct evidence for regeneration of osteoporotic craniomaxillofacial defects remains limited and predominantly preclinical. The most useful translational distinction is whether a strategy has demonstrated defect-level regeneration in an osteoporotic craniomaxillofacial setting, whether it has an established delivery pathway, and whether its added complexity produces a clinically meaningful advantage. Anatomically relevant preclinical evidence should be interpreted as proof of concept rather than as evidence of clinical readiness. Future studies should use clinically matched osteoporotic defect models, report concomitant osteoporosis therapy, and standardize direct regenerative endpoints rather than relying on bone mineral density or mechanistic markers alone. FAU - Aydin, Nur Efsan AU - Aydin NE AUID- ORCID: 0000-0002-0950-3283 AD - Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Gazi University, 06000 Ankara, Turkey. FAU - Ozden, Kerem AU - Ozden K AD - Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Gazi University, 06000 Ankara, Turkey. FAU - Kirkpunar, Alperen AU - Kirkpunar A AD - Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Gazi University, 06000 Ankara, Turkey. FAU - Cetiner, Sedat AU - Cetiner S AUID- ORCID: 0000-0001-8377-0155 AD - Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Gazi University, 06000 Ankara, Turkey. LA - eng PT - Journal Article PT - Review DEP - 20260929 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701715 OTO - NOTNLM OT - biological agents OT - bone regeneration OT - bone tissue engineering OT - craniomaxillofacial defects OT - osteoporosis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/29 CRDT- 2026/10/27 10:35 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/29 00:00 [pmc-release] AID - biomedicines14102204 [pii] AID - biomedicines-14-02204 [pii] AID - 10.3390/biomedicines14102204 [doi] PST - epublish SO - Biomedicines. 2026 Sep 29;14(10):2204. doi: 10.3390/biomedicines14102204. PMID- 42940006 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 29 TI - Survival Outcomes with IL-2-Free Dinutuximab Beta Treatment Strategies With or Without 13-cis-Retinoic Acid in Relapsed or Refractory Neuroblastoma: An Exploratory Unanchored, Population-Adjusted Comparison of Two Prospective Cohorts. LID - 10.3390/biomedicines14102200 [doi] LID - 2200 AB - Background/Objectives: Survival outcomes with IL-2-free dinutuximab beta (DB) treatment strategies with and without 13-cis-retinoic acid (RA) have not been directly compared in a randomized study. We compared event-free survival (EFS) and overall survival (OS) between two study-defined cohorts with relapsed or refractory neuroblastoma receiving DB with or without RA. Methods: We retrospectively reanalyzed individual patient data from APN311-304 study (DB without RA, n = 36) and the experimental arm of APN311-202 V3 study (DB plus RA, n = 44). Eligibility and time zero were harmonized, and overlap-weighted Kaplan-Meier and Cox analyses were performed. An exploratory post-end-of-treatment landmark analysis was conducted among patients who remained alive and EFS-event-free through the landmark. Results: In the primary overlap-weighted analysis, no clear difference was detected between the cohorts for event-free survival (EFS; hazard ratio [HR], 0.92; 95% confidence interval [CI], 0.31-2.73) or overall survival (OS; HR, 1.95; 95% CI, 0.46-8.20). Cohort-specific effective sample sizes were 10.15 for no RA and 15.30 for RA versus nominal sizes of 36 and 44, respectively. Exploratory conditional landmark estimates were also imprecise for EFS (HR, 1.24; 95% CI, 0.37-4.12) and OS (HR, 0.44; 95% CI, 0.07-2.71). Conclusions: This exploratory comparison did not provide evidence of more favorable EFS or OS in the RA cohort than in the no-RA cohort. However, the estimates were highly imprecise and compatible with clinically important benefit or harm. The findings should not be interpreted as evidence of equivalence or as evidence that RA provides no benefit and should not alone guide continuation or omission of RA. Because RA use was perfectly aligned with the source study, the comparison cannot isolate a causal RA effect. FAU - Lode, Holger N AU - Lode HN AUID- ORCID: 0000-0002-1201-208X AD - Department of Pediatric Hematology and Oncology, University Medicine Greifswald, 17475 Greifswald, Germany. FAU - Holko, Przemyslaw AU - Holko P AUID- ORCID: 0000-0002-6821-4078 AD - Department of Nutrition and Drug Research, Institute of Public Health, Faculty of Health Sciences, Jagiellonian University Medical College, 31-008 Krakow, Poland. FAU - Wieczorek, Aleksandra AU - Wieczorek A AD - Department of Pediatric Oncology and Hematology, Institute of Pediatrics, Jagiellonian University Medical College, 31-008 Krakow, Poland. FAU - Sladowska, Katarzyna AU - Sladowska K AD - Department of Nutrition and Drug Research, Institute of Public Health, Faculty of Health Sciences, Jagiellonian University Medical College, 31-008 Krakow, Poland. FAU - Siebert, Nikolai AU - Siebert N AUID- ORCID: 0000-0001-6254-1302 AD - Department of Pediatric Hematology and Oncology, University Medicine Greifswald, 17475 Greifswald, Germany. FAU - Valteau-Couanet, Dominique AU - Valteau-Couanet D AD - Children and Adolescent Oncology Department, Gustave Roussy, Paris-Sud University, 94805 Paris, France. FAU - Garaventa, Alberto AU - Garaventa A AD - Oncology Unit, IRCCS Istituto Giannina Gaslini, 16147 Genoa, Italy. FAU - Canete, Adela AU - Canete A AUID- ORCID: 0000-0002-5669-5097 AD - Hospital Universitario y Politecnico La Fe, 46026 Valencia, Spain. FAU - Anderson, John AU - Anderson J AUID- ORCID: 0000-0001-7509-3203 AD - UCL Great Ormond Street Institute of Child Health, London WC1N 1EH, UK. FAU - Yaniv, Isaac AU - Yaniv I AD - Schneider Children's Medical Center of Israel, Petach Tikva 4920235, Israel. FAU - Ash, Shifra AU - Ash S AD - Schneider Children's Medical Center of Israel, Petach Tikva 4920235, Israel. AD - Adelson School of Medicine, Ariel University, Ariel 4070000, Israel. FAU - Moreno, Lucas AU - Moreno L AD - Division of Pediatric Hematology & Oncology, Vall d'Hebron Barcelona Hospital Campus, Passeig Vall d'Hebron 119, 08035 Barcelona, Spain. FAU - Gray, Juliet AU - Gray J AD - Centre for Cancer Immunology, University of Southampton, Southampton SO16 6YD, UK. FAU - Luksch, Roberto AU - Luksch R AD - Pediatric Oncology Unit, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy. FAU - Laureys, Genevieve AU - Laureys G AUID- ORCID: 0000-0002-5302-4298 AD - Department of Pediatric Hematology/Oncology and Stem Cell Transplantation, Ghent University Hospital, 9000 Ghent, Belgium. FAU - Owens, Cormac AU - Owens C AD - Department of Haematology and Oncology, Children's Health Ireland, D12 N512 Dublin, Ireland. FAU - Manzitti, Carla AU - Manzitti C AUID- ORCID: 0000-0002-1501-5518 AD - Oncology Unit, IRCCS Istituto Giannina Gaslini, 16147 Genoa, Italy. FAU - Troschke-Meurer, Sascha AU - Troschke-Meurer S AUID- ORCID: 0000-0002-6185-462X AD - Department of Pediatric Hematology and Oncology, University Medicine Greifswald, 17475 Greifswald, Germany. FAU - Kawalec, Pawel AU - Kawalec P AD - Department of Nutrition and Drug Research, Institute of Public Health, Faculty of Health Sciences, Jagiellonian University Medical College, 31-008 Krakow, Poland. AD - Centrum HTA Sp. z o.o., 31-232 Krakow, Poland. FAU - Ladenstein, Ruth L AU - Ladenstein RL AD - St. Anna Children's Hospital, St. Anna Children's Cancer Research Institute and Department of Pediatrics, Medical University Vienna, 1090 Vienna, Austria. LA - eng GR - NA/Recordati UK Ltd./ GR - NA/Recordati Rare Diseases/ GR - 5M-2019-23680433/Italian Ministry of Health 5 x 1000 project 2019/ GR - M2116/Ricerca Corrente/ GR - Sl 2147/4-1/Deutsche Forschungsgemeinschaft/ GR - DKS 2024.23/Kinderkrebsstiftung/ PT - Journal Article DEP - 20260929 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701175 OTO - NOTNLM OT - 13-cis-retinoic acid OT - dinutuximab beta OT - event-free survival OT - isotretinoin OT - landmark analysis OT - neuroblastoma OT - overall survival OT - propensity score COIS- Author Pawel Kawalec was employed by the company Centrum HTA Sp. z o.o. H.N.L., A.W., K.S., P.K. and P.H. have acted as consultants and participated in advisory boards organized by EUSA Pharma/Recordati Rare Diseases. J.G. has been a member of a DMC for a trial sponsored by YmAbs Therapeutics and has had previous consulting/advisory board roles for EUSA Pharma, YmAbs Therapeutics, Celgene, Norgine, and AbbVie. R.L. (Roberto Luksch) received travel reimbursement from Recordati Rare Diseases. L.M. is a past member of data monitoring committees for clinical trials sponsored by Novartis, Shionogi, and Incyte; has had consulting roles for Novartis, Norgine, Boehringer, YmAbs, Merck, Roche, Bayer, and Shionogi; has participated in educational activities organized by Bayer and EUSA Pharma and received travel expenses from EUSA Pharma; and is President of SIOPEN (European neuroblastoma research cooperative group), an organization that receives royalties from sales of dinutuximab beta. Recordati UK Ltd. provided funding for data extraction and editorial assistance, and Recordati Rare Diseases funded the open-access publication fee. These funders had no role in the study design, selection of data sources, data access, statistical analysis, interpretation of the results, decision to submit the manuscript for publication, or choice of journal. Although editorial assistance was funded by Recordati UK Ltd., the funder had no control over the scientific content, interpretation, conclusions, or final wording of the manuscript. The authors retained full responsibility for the conduct of the analysis, the integrity and interpretation of the results, the content of the manuscript, and the decision to publish. The other funders listed in the Funding section had no role in the design, conduct, analysis, interpretation, or preparation of the study. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/29 CRDT- 2026/10/27 10:35 PHST- 2026/08/05 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/29 00:00 [pmc-release] AID - biomedicines14102200 [pii] AID - biomedicines-14-02200 [pii] AID - 10.3390/biomedicines14102200 [doi] PST - epublish SO - Biomedicines. 2026 Sep 29;14(10):2200. doi: 10.3390/biomedicines14102200. PMID- 42940005 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 28 TI - Selective Dopamine Transporter Inhibitor CE-123 Modulates Region-Specific Intestinal Mucosal and Immune Responses Following Fear Conditioning and Extinction in Adolescent Female Rats. LID - 10.3390/biomedicines14102199 [doi] LID - 2199 AB - Background/Objectives: Post-traumatic stress disorder (PTSD) is associated with systemic alterations that may involve the gastrointestinal tract, yet intestinal responses to fear-related learning remain poorly characterized. We investigated intestinal mucosal morphology, MUC2 immunoreactivity, and local immune responses following fear conditioning and extinction, and assessed their modulation by the selective dopamine transporter inhibitor CE-123. Methods: Twenty-four adolescent female Wistar rats were assigned to Control or fear-conditioned (FC) groups receiving Vehicle or CE-123 (10 mg/kg, i.p.) during the extinction phase. Morphometry and immunohistochemical detection of CD68, CD206, iNOS, IL-6, IL-10, and MUC2 were evaluated in the duodenum, jejunum, ileum, and colon. Results: Fear conditioning was associated with marked region-dependent alterations. Among Vehicle-treated animals, villus height was increased in the duodenum and jejunum, accompanied by enhanced immunoreactivity of several inflammation-associated markers. In contrast, the ileum and colon showed distinct profiles characterized mainly by increased IL-6, reduced selected immunoregulatory markers, and decreased MUC2. CE-123 in fear conditioning (FC) showed its clearest attenuation of inflammation-associated responses in the jejunum, where CD68, iNOS, and IL-6 immunoreactivity were reduced. In comparison, this pattern was not consistently observed in other intestinal regions: in the duodenum, only CD68 immunoreactivity was reduced, whereas in the ileum, a reduction was observed only for IL-6. In the colon, both IL-6 and CD68 showed changes in the opposite direction to those observed in the jejunum. Collectively, these region-specific and divergent responses suggest that CE-123 exerts a modulatory rather than uniformly anti-inflammatory effect on intestinal immune responses. CE-123 also produced independent effects in Control animals, including segment-specific changes in mucosal morphometry, MUC2, and selected immune markers, indicating that its actions were not limited to attenuation of conditioning-associated alterations. Conclusions: These findings demonstrate segment-specific intestinal remodeling following fear conditioning and extinction and indicate a context-dependent immunomodulatory effect of CE-123. FAU - Kras, Katarzyna AU - Kras K AUID- ORCID: 0000-0002-7790-3780 AD - Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin, Poland. FAU - Manastyrska-Stolarczyk, Malgorzata AU - Manastyrska-Stolarczyk M AD - Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin, Poland. FAU - Arciszewski, Kamil AU - Arciszewski K AUID- ORCID: 0000-0002-7200-3580 AD - Clinical Neurology Ward, The University Clinical Hospital No. 4 in Lublin, Jaczewskiego 8, 20-090 Lublin, Poland. FAU - Osiak-Wicha, Cezary AU - Osiak-Wicha C AUID- ORCID: 0000-0001-5630-4968 AD - Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin, Poland. FAU - Grochecki, Pawel AU - Grochecki P AUID- ORCID: 0000-0003-0591-3645 AD - Department of Pharmacology and Pharmacodynamics, Medical University of Lublin, Chodzki 4a, 20-093 Lublin, Poland. FAU - Kotlinska, Jolanta H AU - Kotlinska JH AUID- ORCID: 0000-0002-4178-1515 AD - Department of Pharmacology and Pharmacodynamics, Medical University of Lublin, Chodzki 4a, 20-093 Lublin, Poland. FAU - Slowik, Tymoteusz AU - Slowik T AD - Experimental Medicine Center, Medical University of Lublin, Jaczewskiego 8, 20-090 Lublin, Poland. FAU - Lubec, Gert AU - Lubec G AD - Department of Neuroproteomics, Paracelsus Medical University, 5020 Salzburg, Austria. FAU - Arciszewski, Marcin B AU - Arciszewski MB AUID- ORCID: 0000-0002-3643-3524 AD - Department of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin, Poland. LA - eng PT - Journal Article DEP - 20260928 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701837 OTO - NOTNLM OT - CE-123 OT - MUC2 OT - dopamine transporter OT - fear conditioning OT - fear extinction OT - intestinal mucosa OT - mucosal immune response OT - post-traumatic stress disorder COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/28 CRDT- 2026/10/27 10:35 PHST- 2026/09/07 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/27 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - biomedicines14102199 [pii] AID - biomedicines-14-02199 [pii] AID - 10.3390/biomedicines14102199 [doi] PST - epublish SO - Biomedicines. 2026 Sep 28;14(10):2199. doi: 10.3390/biomedicines14102199. PMID- 42940004 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 28 TI - Integrated Transcriptomic Analysis Reveals Distinct Molecular and Immune Microenvironment Features of Peutz-Jeghers Syndrome-Associated Lobular Endocervical Glandular Hyperplasia and Gastric-Type Endocervical Adenocarcinoma. LID - 10.3390/biomedicines14102197 [doi] LID - 2197 AB - Background: Gastric-type endocervical adenocarcinoma (GAS) is an aggressive, human papillomavirus-independent cervical adenocarcinoma, whereas lobular endocervical glandular hyperplasia (LEGH) is considered a potential precursor. Molecular and immune microenvironment differences between Peutz-Jeghers syndrome-associated LEGH (PJS-LEGH) and GAS (PJS-GAS) remain poorly characterized. This study aimed to characterize these differences through integrated transcriptomic analysis. Methods: Bulk RNA sequencing was performed on tissues from 7 PJS-LEGH and 8 PJS-GAS cases. Differential expression and functional enrichment analyses were integrated with weighted gene co-expression network analysis (WGCNA), molecular interaction network analysis, and machine learning. Immune-related transcriptional patterns were assessed using single-sample gene set enrichment analysis (ssGSEA) and xCell, with CD45/CD4 multiplex immunofluorescence for preliminary tissue-level assessment. Results: We identified 1794 differentially expressed genes, including 976 upregulated and 818 downregulated genes in PJS-GAS. Functional and network analyses highlighted differences involving ciliary biology, epithelial differentiation, chemokine signaling, and immune regulation. ssGSEA revealed higher scores for selected T-cell, dendritic-cell, monocyte, and myeloid-derived suppressor cell signatures in PJS-GAS. xCell provided complementary evidence of higher scores for selected immune-cell populations, together with activation- and inhibition-associated transcriptional signatures. Integrated analyses converged on PTPRC, encoding the pan-leukocyte marker CD45, as a prominent leukocyte-associated feature of PJS-GAS. Multiplex immunofluorescence showed higher CD45(+) and CD4(+) cell densities in the sampled PJS-GAS tissues. Conclusions: This study provides the first transcriptomic comparison of PJS-LEGH and PJS-GAS and identifies distinct molecular features and increased immune-cell-associated transcriptional signatures with coexisting activation- and inhibition-associated expression patterns in PJS-GAS. FAU - Gong, Xiaoqin AU - Gong X AD - Department of Obstetrics and Gynecology, Shanghai Key Laboratory of Reproduction and Development, Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200090, China. FAU - Chen, Yiqing AU - Chen Y AD - Department of Obstetrics and Gynecology, Shanghai Key Laboratory of Reproduction and Development, Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200090, China. FAU - Jiang, Anqi AU - Jiang A AD - Department of Obstetrics and Gynecology, Shanghai Key Laboratory of Reproduction and Development, Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200090, China. FAU - Ye, Yingfei AU - Ye Y AD - Department of Obstetrics and Gynecology, Shanghai Key Laboratory of Reproduction and Development, Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200090, China. FAU - Wang, Hui AU - Wang H AD - Phase One Clinical Trials Unit, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200090, China. FAU - Chai, Ranran AU - Chai R AD - Department of Obstetrics and Gynecology, Shanghai Key Laboratory of Reproduction and Development, Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200090, China. FAU - Xu, Jing AU - Xu J AD - Department of Obstetrics and Gynecology, Shanghai Key Laboratory of Reproduction and Development, Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200090, China. FAU - Jin, Guanqin AU - Jin G AD - Phase One Clinical Trials Unit, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200090, China. FAU - Zhang, Hongwei AU - Zhang H AD - Department of Obstetrics and Gynecology, Shanghai Key Laboratory of Reproduction and Development, Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200090, China. FAU - Tong, Jinyi AU - Tong J AUID- ORCID: 0000-0003-2576-8764 AD - Department of Gynecology, Affiliated Hangzhou First People's Hospital, School of Medicine, Westlake University, Hangzhou 310006, China. FAU - Kang, Yu AU - Kang Y AD - Department of Obstetrics and Gynecology, Shanghai Key Laboratory of Reproduction and Development, Shanghai Key Laboratory of Female Reproductive Endocrine-Related Diseases, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200090, China. AD - Phase One Clinical Trials Unit, Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200090, China. AD - Department of Gynecology, Affiliated Hangzhou First People's Hospital, School of Medicine, Westlake University, Hangzhou 310006, China. LA - eng GR - 22MC1940200/Shanghai Clinical Research Center for Gynecological Diseases/ GR - 2022YFC2705004/the National Key Research and Development Program of China/ GR - BJWS2025027/the Shanghai Oriental Talents Program-Outstanding Projects/ PT - Journal Article DEP - 20260928 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701942 OTO - NOTNLM OT - Peutz-Jeghers syndrome OT - gastric-type endocervical adenocarcinoma OT - immune microenvironment OT - lobular endocervical glandular hyperplasia OT - transcriptomic analysis OT - weighted gene co-expression network analysis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/28 CRDT- 2026/10/27 10:35 PHST- 2026/08/11 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - biomedicines14102197 [pii] AID - biomedicines-14-02197 [pii] AID - 10.3390/biomedicines14102197 [doi] PST - epublish SO - Biomedicines. 2026 Sep 28;14(10):2197. doi: 10.3390/biomedicines14102197. PMID- 42940002 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 28 TI - Antimicrobial Resistance Patterns Among Gram-Negative Bacterial Isolates Recovered from Tracheal Aspirate Cultures in Turkiye: A Systematic Review and Meta-Analysis. LID - 10.3390/biomedicines14102196 [doi] LID - 2196 AB - Background: This systematic review and meta-analysis aimed to evaluate antimicrobial resistance patterns among selected Gram-negative bacterial isolates recovered from tracheal aspirate cultures in Turkiye. Methods: The systematic review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Electronic databases were systematically searched to identify eligible studies reporting antimicrobial resistance patterns among selected Gram-negative bacterial isolates from tracheal aspirate cultures in Turkiye. Random-effects meta-analyses of study-level resistance proportions were performed using the number of resistant and tested isolates reported by each study. Between-study heterogeneity was assessed using Cochran's Q test and the I(2) statistic. Sensitivity analyses and exploratory univariable meta-regression analyses were also performed. Results: Eighteen studies comprising 5436 isolates belonging to five selected Gram-negative bacterial groups recovered from tracheal aspirate cultures were included. The pooled meropenem resistance rates were 93.21% for A. baumannii, 37.7% for P. aeruginosa, 29.3% for K. pneumoniae, and 4.3% for E. coli. Pseudomonas aeruginosa showed a higher pooled meropenem resistance estimate than K. pneumoniae, while both species exhibited substantial resistance to several other commonly used antimicrobial agents. Exploratory meta-regression indicated an increase in meropenem resistance across more recent study periods for K. pneumoniae, whereas no significant temporal trend was observed for A. baumannii or P. aeruginosa. Conclusions: Multidrug-resistant Gram-negative bacteria continue to represent a major problem in tracheal aspirate cultures in Turkiye. In particular, the high prevalence of carbapenem resistance in A. baumannii highlights the need for continuous national surveillance, standardized microbiological methods, strengthened antimicrobial stewardship, and effective infection prevention and control measures. FAU - Kahraman Kilbas, Elmas Pinar AU - Kahraman Kilbas EP AUID- ORCID: 0000-0003-1348-625X AD - Medical Laboratory Techniques Program, Vocational School of Health Services, Fenerbahce University, Istanbul 34758, Turkiye. FAU - Ciftci, Ihsan Hakki AU - Ciftci IH AD - Department of Medical Microbiology, Faculty of Medicine, Sakarya University, Sakarya 54100, Turkiye. FAU - Kilbas, Imdat AU - Kilbas I AD - Dental Prosthesis Technology Program, Vocational School of Health Services, Fenerbahce University, Istanbul 34758, Turkiye. FAU - Didbaridze, Tamar AU - Didbaridze T AD - Department of Microbiology, Tbilisi State Medical University, Tbilisi 0186, Georgia. LA - eng PT - Journal Article PT - Review DEP - 20260928 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701897 OTO - NOTNLM OT - Acinetobacter baumannii OT - Klebsiella pneumoniae OT - Pseudomonas aeruginosa OT - drug resistance OT - prevalence OT - tracheal aspirate COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/28 CRDT- 2026/10/27 10:35 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - biomedicines14102196 [pii] AID - biomedicines-14-02196 [pii] AID - 10.3390/biomedicines14102196 [doi] PST - epublish SO - Biomedicines. 2026 Sep 28;14(10):2196. doi: 10.3390/biomedicines14102196. PMID- 42939994 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 27 TI - Exploratory Integration of Metabolomics and Machine Learning Identifies Candidate Signatures of Spontaneous Preterm Birth. LID - 10.3390/biomedicines14102188 [doi] LID - 2188 AB - Background/Objectives: Spontaneous preterm birth (sPTB) remains a major cause of neonatal morbidity and mortality. This study aimed to characterize first-trimester plasma metabolic alterations associated with sPTB and evaluate their discriminatory potential using metabolomics and machine learning. Methods: This retrospective case-control study included 199 pregnant women (64 sPTB and 135 term births) from the Colombian Cohort for the Early Prediction of Preterm Birth (COLPRET). First-trimester plasma was analyzed by Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (LC-QTOF-MS). Multivariate and univariate analyses were used to characterize metabolic differences. Thirty candidate metabolites were selected through outcome-associated univariate analyses performed on the complete cohort before subsequent machine-learning model evaluation. Six machine-learning algorithms were evaluated using clinical, metabolomic, and integrated predictor sets within a nested stratified cross-validation framework. Results: Lipid-related metabolites, particularly lysophospholipids and phospholipids, predominated among the molecular alterations associated with sPTB. Lysophosphatidylcholine O-14:0 (LPC O-14:0) showed the highest individual discrimination with an area under the receiver operating characteristic curve (ROC-AUC) (AUC = 0.705). Models based on clinical data showed limited discrimination (out-of-fold [OOF] ROC-AUC: 0.500-0.555), whereas metabolomic and integrated models achieved OOF ROC-AUC values of 0.632-0.760 and 0.641-0.770, respectively. Metabolomic and integrated models generally showed higher ROC-AUC estimates than clinical models. However, adding the five clinical variables to the metabolomic predictor set did not significantly improve the ROC-AUC after correction for multiple comparisons (all false discovery rate [FDR]-adjusted q >/= 0.060). Conclusions: First-trimester plasma metabolomics identified predominantly lipid-related alterations associated with subsequent sPTB. Metabolomic information showed greater discriminatory capacity than the five evaluated clinical variables, with no robust evidence of additional discrimination from their integration. These exploratory findings require validation in larger prospective cohorts before clinical utility can be established. FAU - Avellaneda-Arciniegas, Maicol AU - Avellaneda-Arciniegas M AD - School of Medicine, Universidad Industrial de Santander, Bucaramanga 680002, Colombia. FAU - Becerra-Mojica, Carlos H AU - Becerra-Mojica CH AUID- ORCID: 0000-0003-3188-530X AD - School of Medicine, Universidad Industrial de Santander, Bucaramanga 680002, Colombia. FAU - Cala, Monica P AU - Cala MP AUID- ORCID: 0000-0002-8198-726X AD - MetCore-Metabolomics Core Facility, Vice-Presidency for Research, Universidad de los Andes, Bogota 111711, Colombia. FAU - Rincon-Orozco, Bladimiro AU - Rincon-Orozco B AUID- ORCID: 0000-0001-5323-0422 AD - School of Medicine, Universidad Industrial de Santander, Bucaramanga 680002, Colombia. FAU - Hidalgo, William AU - Hidalgo W AUID- ORCID: 0000-0002-9973-2679 AD - School of Chemistry, Universidad Industrial de Santander, Bucaramanga 680002, Colombia. LA - eng GR - 652/Ministerio de Ciencia, Tecnologia e Innovacion/ PT - Journal Article DEP - 20260927 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701544 OTO - NOTNLM OT - first trimester OT - lipids OT - machine learning OT - metabolomic OT - plasma OT - spontaneous preterm birth COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/27 CRDT- 2026/10/27 10:35 PHST- 2026/08/22 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - biomedicines14102188 [pii] AID - biomedicines-14-02188 [pii] AID - 10.3390/biomedicines14102188 [doi] PST - epublish SO - Biomedicines. 2026 Sep 27;14(10):2188. doi: 10.3390/biomedicines14102188. PMID- 42939993 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 27 TI - CD47-SIRPalpha as a Therapeutic Target in Sarcoma: A Systematic Review of Expression, Preclinical, and Clinical Evidence. LID - 10.3390/biomedicines14102187 [doi] LID - 2187 AB - Background/Objectives: Sarcoma comprises mesenchymal malignancies for which PD-1/PD-L1 checkpoint inhibitors have produced few durable responses. The CD47-SIRPalpha innate checkpoint suppresses macrophage phagocytosis through a "don't eat me" signal and is the most clinically advanced myeloid checkpoint, but its evidence base in sarcoma has not been systematically assembled or appraised. Methods: A PRISMA 2020-compliant systematic review (PROSPERO CRD420261452040) was undertaken; three bibliographic databases and three trial registers, without language restriction, were searched on 14 September 2026. Expression, preclinical and clinical studies were appraised and graded separately. Synthesis followed SWiM guidelines and no pooled effect estimate was calculated. Results: Of 391 records identified, 50 studies met the eligibility criteria as separate analysis units (45 journal articles, 4 conference abstracts, 1 registry record), appraised across expression (20), preclinical (38) and clinical (18, including 2 interventional trials) domains. CD47 expression was subtype-dependent and bimodal, consistent in chordoma and angiosarcoma, frequently negative in leiomyosarcoma and Ewing sarcoma, and contested in undifferentiated pleomorphic sarcoma; reported positivity in osteosarcoma varied six-fold between cohorts, and the assay was often incompletely described. In preclinical models, CD47 blockade consistently increased phagocytosis and combination regimens outperformed single-agent use, but few studies used patient-derived or humanized models. Only two interventional trials enrolled sarcoma patients; both were single-arm, one was terminated without posted results, and nine of ten pharmacological strategies had no sarcoma-specific clinical data. Certainty was low for expression and patient-cohort evidence, and very low for preclinical and interventional evidence. Conclusions: The CD47-SIRPalpha axis is biologically well characterized and expressed in a definable subset of sarcomas, but has not been tested in these tumors in a design capable of detecting a treatment effect. Four of the fifty records were conference abstracts, which carry a higher risk of reporting bias and non-publication. The rate-limiting step lies in the assay and the trial architecture rather than in the biology: a harmonized immunohistochemical standard and a biomarker-selected trial with an internal comparator are prerequisites for an informative next study. FAU - Xiao, Misu AU - Xiao M AUID- ORCID: 0000-0001-6847-4972 AD - Department of Pharmacy, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China. FAU - Guo, Cheng AU - Guo C AD - Department of Pharmacy, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China. FAU - Yang, Quanjun AU - Yang Q AD - Department of Pharmacy, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China. LA - eng GR - 21PJ1411900/Science and Technology Commission of Shanghai Municipality/ GR - Shanghai Sixth People's Hospital/ PT - Journal Article PT - Review DEP - 20260927 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701423 OTO - NOTNLM OT - CD47 OT - SIRPalpha OT - innate immune checkpoint OT - macrophage phagocytosis OT - sarcoma OT - tumor-associated macrophage OT - "don't eat me" signal COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/27 CRDT- 2026/10/27 10:35 PHST- 2026/07/23 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - biomedicines14102187 [pii] AID - biomedicines-14-02187 [pii] AID - 10.3390/biomedicines14102187 [doi] PST - epublish SO - Biomedicines. 2026 Sep 27;14(10):2187. doi: 10.3390/biomedicines14102187. PMID- 42939992 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 27 TI - SEMA3A in Proliferative Vitreoretinopathy: Exogenous Activity, Endogenous Regulation, and the MicroRNA Hypothesis. LID - 10.3390/biomedicines14102186 [doi] LID - 2186 AB - Proliferative vitreoretinopathy (PVR) remains a leading cause of surgical failure after rhegmatogenous retinal detachment (RRD) repair. Although PVR involves multiple cell types and signaling pathways, epithelial-mesenchymal transition (EMT) and related plasticity of retinal pigment epithelial (RPE) cells are major contributors to fibrocellular membrane formation and contraction. Semaphorin 3A (SEMA3A) has therefore attracted interest as a potential negative regulator of these pathogenic RPE responses. Recombinant SEMA3A inhibits RPE migration and proliferation and attenuates experimental PVR when administered at model induction. Yet this benefit does not establish that endogenous SEMA3A transcript levels are reduced. Published Source Data from a mouse model of traumatic PVR show significantly increased endogenous Sema3a transcript levels in RPE preparations compared with controls. The question is therefore why PVR progresses despite increased endogenous Sema3a transcript levels in this model. Possible explanations include microRNA-mediated translational repression, altered protein processing or secretion, reduced extracellular availability, and impaired NRP1/plexin signaling. MicroRNAs (miRNAs) are particularly relevant because they can reduce protein output without a parallel decline in mRNA and can regulate multiple features of RPE EMT. This narrative review examines current evidence for SEMA3A in PVR, summarizes miRNA regulation of RPE EMT and related PVR phenotypes, and evaluates direct and indirect evidence for miRNA-SEMA3A interactions. We also compare the miRNA hypothesis with alternative explanations for inadequate endogenous SEMA3A activity and consider its implications for ocular biomarkers and target-site-selective RNA therapy. Current evidence makes miRNA-mediated regulation of SEMA3A a plausible and testable mechanism, although a causal role has yet to be demonstrated in RPE cells or PVR. FAU - Wang, Bohao AU - Wang B AD - Department of Ophthalmology, Peking University People's Hospital, Beijing 100044, China. AD - Beijing Key Laboratory of Ocular Disease and Optometry Science, Peking University People's Hospital, Beijing 100044, China. FAU - Li, Siying AU - Li S AD - Department of Ophthalmology, Peking University People's Hospital, Beijing 100044, China. AD - Beijing Key Laboratory of Ocular Disease and Optometry Science, Peking University People's Hospital, Beijing 100044, China. FAU - Cao, Yu AU - Cao Y AD - Department of Ophthalmology, Peking University People's Hospital, Beijing 100044, China. AD - Beijing Key Laboratory of Ocular Disease and Optometry Science, Peking University People's Hospital, Beijing 100044, China. FAU - Sun, Aimin AU - Sun A AD - Department of Ophthalmology, Peking University People's Hospital, Beijing 100044, China. AD - Beijing Key Laboratory of Ocular Disease and Optometry Science, Peking University People's Hospital, Beijing 100044, China. FAU - Li, Muzi AU - Li M AD - Department of Ophthalmology, Peking University People's Hospital, Beijing 100044, China. AD - Beijing Key Laboratory of Ocular Disease and Optometry Science, Peking University People's Hospital, Beijing 100044, China. FAU - Qu, Jinfeng AU - Qu J AUID- ORCID: 0000-0001-6875-8393 AD - Department of Ophthalmology, Peking University People's Hospital, Beijing 100044, China. AD - Beijing Key Laboratory of Ocular Disease and Optometry Science, Peking University People's Hospital, Beijing 100044, China. LA - eng PT - Journal Article PT - Review DEP - 20260927 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701372 OTO - NOTNLM OT - SEMA3A OT - epithelial plasticity OT - epithelial-mesenchymal transition OT - miRNA therapeutics OT - microRNA OT - molecular staging OT - ocular-fluid biomarkers OT - precision medicine OT - proliferative vitreoretinopathy OT - retinal pigment epithelium COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/27 CRDT- 2026/10/27 10:35 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - biomedicines14102186 [pii] AID - biomedicines-14-02186 [pii] AID - 10.3390/biomedicines14102186 [doi] PST - epublish SO - Biomedicines. 2026 Sep 27;14(10):2186. doi: 10.3390/biomedicines14102186. PMID- 42939989 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 26 TI - Personalizing Inhaled Therapy in COPD and Asthma: Integrating Drug, Device, and Patient Factors. LID - 10.3390/biomedicines14102184 [doi] LID - 2184 AB - The management of chronic obstructive pulmonary disease (COPD) and asthma depends largely on the effective delivery of aerosolized medications to the respiratory tract. Clinical efficacy is determined not only by the active pharmaceutical ingredient but also by the interplay among drug formulation, aerosol particle size, inhaler device characteristics, dosing frequency, and patient-specific factors. This review examines the major inhaled therapeutic platforms, including soft mist inhalers (SMIs), pressurized metered-dose inhalers (pMDIs), and dry powder inhalers (DPIs), and evaluates how their characteristics influence pulmonary drug delivery. We examine the impact of particle size and extrafine formulations on peripheral deposition and small airway disease (SAD), as well as clinically relevant factors including peak inspiratory flow rate (PIFR), inhaled corticosteroid-associated pneumonia risk, dosing frequency and adherence, rescue inhaler requirements, inhaler technique, cost and accessibility, and environmental impact. By integrating inhaler characteristics with real-world clinical considerations, this article provides a structured, evidence-based framework for individualized inhaler selection in obstructive lung disease. However, the available evidence is limited by heterogeneity across devices, formulations, study populations, and assessment methods, highlighting the need for further comparative effectiveness studies and evaluation of emerging inhaler technologies. FAU - Yung, Kenneth AU - Yung K AD - Department of Internal Medicine, Far Eastern Memorial Hospital, New Taipei City 220, Taiwan. FAU - Cheng, Shih-Lung AU - Cheng SL AD - Department of Internal Medicine, Far Eastern Memorial Hospital, New Taipei City 220, Taiwan. AD - Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan 320, Taiwan. LA - eng PT - Journal Article PT - Review DEP - 20260926 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701278 OTO - NOTNLM OT - COPD OT - asthma OT - inhaler devices OT - peak inspiratory flow rate OT - pharmaceutical aerosols OT - small airway disease COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/26 CRDT- 2026/10/27 10:35 PHST- 2026/08/13 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/26 00:00 [pmc-release] AID - biomedicines14102184 [pii] AID - biomedicines-14-02184 [pii] AID - 10.3390/biomedicines14102184 [doi] PST - epublish SO - Biomedicines. 2026 Sep 26;14(10):2184. doi: 10.3390/biomedicines14102184. PMID- 42939983 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 25 TI - Photothrombotic Ischemic Stroke in Rats: A Systematic Review of Experimental Parameters and Therapeutic Applications in Neuroprotection, Neuromodulation, and Post-Stroke Recovery. LID - 10.3390/biomedicines14102177 [doi] LID - 2177 AB - Background/Objectives: This systematic review analyzed the experimental parameters of the photothrombotic ischemic stroke model in rats and their relationship with neuroprotection, neuromodulation, and post-stroke recovery studies. We examined the follow-up periods and the behavioral, histological, and complementary outcomes employed. Methods: The search was conducted in PubMed and Scopus following PRISMA guidelines. Of the 341 records identified, 34 studies met the eligibility criteria. Results: Sprague-Dawley, male, and young adult rats predominated, while Rose Bengal was used in all studies. Intravenous administration and transcranial irradiation without craniotomy were the most frequent approaches, with predominantly unilateral lesions targeting motor and sensorimotor regions. Despite this relative uniformity, the considerable variability in irradiation parameters and incomplete reporting of relevant physical characteristics of the protocols were observed, indicating limitations to interlaboratory reproducibility. Post-stroke recovery was the most frequent application, followed by neuromodulation and neuroprotection. Neuroprotection studies were concentrated in earlier time windows and combined a functional assessment with the characterization of lesion, neuronal survival, cell death, oxidative stress, and inflammation. Neuromodulation studies showed greater diversity in stimulation strategies and integrated behavioral outcomes with cellular, neurovascular, perfusion, neuroimaging, and electrophysiological assessments, supporting the investigation of circuit modulation. In contrast, recovery studies employed longer follow-up periods, with greater emphasis on dexterity, asymmetry, and motor performance, associated with assessments of neurogenesis, angiogenesis, neurotrophic factors, and neuronal reorganization. Conclusions: Overall, the findings demonstrate that neuroprotection, neuromodulation, and post-stroke recovery require distinct temporal windows and assessment strategies, and that the integrated selection of induction parameters and outcomes should be guided by the therapeutic purpose to improve the consistency and comparability of studies using the photothrombotic model. FAU - Raci, Giovanna R AU - Raci GR AUID- ORCID: 0009-0006-0847-2119 AD - Hospital Israelita Albert Einstein, Sao Paulo 05652-000, SP, Brazil. FAU - Oliveira, Fernando A AU - Oliveira FA AUID- ORCID: 0000-0002-7226-1694 AD - Hospital Israelita Albert Einstein, Sao Paulo 05652-000, SP, Brazil. FAU - Valle, Nicole M E AU - Valle NME AUID- ORCID: 0000-0003-4523-1753 AD - Hospital Israelita Albert Einstein, Sao Paulo 05652-000, SP, Brazil. FAU - Dias, Olivia F M AU - Dias OFM AD - Hospital Israelita Albert Einstein, Sao Paulo 05652-000, SP, Brazil. FAU - Alves, Arielly H AU - Alves AH AUID- ORCID: 0000-0003-3570-0827 AD - Hospital Israelita Albert Einstein, Sao Paulo 05652-000, SP, Brazil. FAU - Mamani, Javier B AU - Mamani JB AD - Hospital Israelita Albert Einstein, Sao Paulo 05652-000, SP, Brazil. FAU - Dall'Ovo, Bruna O AU - Dall'Ovo BO AUID- ORCID: 0009-0004-1991-7182 AD - Hospital Israelita Albert Einstein, Sao Paulo 05652-000, SP, Brazil. FAU - Tatai, Beatriz A AU - Tatai BA AUID- ORCID: 0009-0000-0944-0318 AD - Hospital Israelita Albert Einstein, Sao Paulo 05652-000, SP, Brazil. FAU - Nucci, Mariana P AU - Nucci MP AUID- ORCID: 0000-0002-1502-9215 AD - LIM44, Hospital das Clinicas, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 05403-000, SP, Brazil. FAU - Gamarra, Lionel F AU - Gamarra LF AUID- ORCID: 0000-0002-3910-0047 AD - Hospital Israelita Albert Einstein, Sao Paulo 05652-000, SP, Brazil. LA - eng GR - 307318/2023-0, 407003/2025-8, 102035/2024-5/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ GR - 2023/10843-7, 2025/12479-6, 2026/07396-7, 2025/26135-7/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/ GR - 442539/2019-3/SisNANO 2.0/MCTIC/ PT - Journal Article PT - Review DEP - 20260925 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701995 OTO - NOTNLM OT - animal behavior OT - ischemic stroke OT - neuromodulation OT - neuroprotection OT - post-stroke recovery COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/25 CRDT- 2026/10/27 10:35 PHST- 2026/08/28 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - biomedicines14102177 [pii] AID - biomedicines-14-02177 [pii] AID - 10.3390/biomedicines14102177 [doi] PST - epublish SO - Biomedicines. 2026 Sep 25;14(10):2177. doi: 10.3390/biomedicines14102177. PMID- 42939982 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 25 TI - Association of the Charlson Comorbidity Index and Age-Adjusted Charlson Comorbidity Index with Clinical Course and Mortality in Patients with Idiopathic Pulmonary Fibrosis. LID - 10.3390/biomedicines14102176 [doi] LID - 2176 AB - Background/Objectives: Idiopathic pulmonary fibrosis (IPF) frequently coexists with comorbidities that may influence clinical outcomes. The Charlson Comorbidity Index (CCI) quantifies the weighted comorbidity burden, whereas the age-adjusted Charlson Comorbidity Index (ACCI) additionally incorporates age. We evaluated the associations of CCI and ACCI with pulmonary function, disease severity, clinical course, and mortality in IPF. Methods: This retrospective, single-center cohort study included 319 patients with IPF. Comorbidities present at diagnosis were scored using the CCI and ACCI. Associations with pulmonary function, GAP index, acute exacerbations, hospitalization, long-term oxygen therapy, echocardiographic findings suggestive of pulmonary hypertension, incident malignancy, antifibrotic treatment, and mortality were assessed. Correlation analyses were performed using Spearman's rank correlation coefficient, and Bonferroni correction was applied to account for multiple testing. Multivariable Cox proportional hazards regression was used to evaluate whether the CCI or ACCI was independently associated with survival. Time-to-event analyses were performed using Kaplan-Meier survival analysis. Results: The mean age was 71 +/- 8 years, 78.1% were male, and 75.5% had at least one comorbid condition among patients with available comorbidity data. The median CCI and ACCI scores were 2 (IQR, 0-4) and 5 (IQR, 4-7), respectively. Higher CCI and ACCI categories were associated with hospitalization (p = 0.006 and p = 0.011), echocardiographic findings suggestive of pulmonary hypertension (both p = 0.002), and incident malignancy (p < 0.001 and p = 0.005, respectively). After Bonferroni correction, higher CCI and ACCI categories remained significantly associated with echocardiographic findings suggestive of pulmonary hypertension (both p = 0.002) and incident malignancy (both p < 0.001), whereas the associations with hospitalization did not remain statistically significant. A nominal association between ACCI and mortality (p = 0.044) also did not remain significant after Bonferroni correction. In Spearman analyses, ACCI remained significantly correlated with current age, age at IPF diagnosis, and the GAP index after correction, whereas the correlations of CCI and ACCI with baseline DLCO did not. Neither CCI (HR = 1.029, 95% CI: 0.923-1.146; p = 0.610) nor ACCI (HR = 1.035, 95% CI: 0.936-1.144; p = 0.509) was independently associated with survival in multivariable Cox regression analyses. Lower baseline DLCO remained independently associated with survival in both Cox models (both p < 0.001).Kaplan-Meier survival curves were constructed according to the predefined CCI and ACCI score categories (0, 1-2, 3-4, and >/=5), and survival distributions across categories were compared using the log-rank test. Conclusions: CCI and ACCI were associated with selected clinical characteristics in IPF, particularly echocardiographic findings suggestive of pulmonary hypertension and incident malignancy. However, neither index independently predicted survival after multivariable adjustment. These indices may complement, rather than replace, disease-specific physiological assessments in patients with IPF. FAU - Onyilmaz, Tugba AU - Onyilmaz T AUID- ORCID: 0000-0001-7022-4577 AD - Department of Pulmonary Medicine, Faculty of Medicine, Kocaeli University, 41380 Kocaeli, Turkey. FAU - Oksuzler Kizilbay, Gozde AU - Oksuzler Kizilbay G AUID- ORCID: 0000-0002-6462-7094 AD - Department of Pulmonary Medicine, Private Konak Hospital, 41100 Kocaeli, Turkey. FAU - Kaya, Huseyin AU - Kaya H AUID- ORCID: 0000-0002-8838-9502 AD - Department of Pulmonary Medicine, Faculty of Medicine, Kocaeli University, 41380 Kocaeli, Turkey. FAU - Argun Baris, Serap AU - Argun Baris S AUID- ORCID: 0000-0002-4429-9441 AD - Department of Pulmonary Medicine, Faculty of Medicine, Kocaeli University, 41380 Kocaeli, Turkey. FAU - Ozturk, Bengugul AU - Ozturk B AUID- ORCID: 0009-0007-5406-0454 AD - Department of Pulmonary Medicine, Faculty of Medicine, Kocaeli University, 41380 Kocaeli, Turkey. FAU - Boyaci, Hasim AU - Boyaci H AD - Department of Pulmonary Medicine, Faculty of Medicine, Kocaeli University, 41380 Kocaeli, Turkey. FAU - Basyigit, Ilknur AU - Basyigit I AUID- ORCID: 0000-0001-7706-9311 AD - Department of Pulmonary Medicine, Faculty of Medicine, Kocaeli University, 41380 Kocaeli, Turkey. LA - eng PT - Journal Article DEP - 20260925 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701863 OTO - NOTNLM OT - Charlson Comorbidity Index OT - age-adjusted Charlson Comorbidity Index OT - clinical outcomes OT - comorbidity OT - idiopathic pulmonary fibrosis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/25 CRDT- 2026/10/27 10:35 PHST- 2026/08/27 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - biomedicines14102176 [pii] AID - biomedicines-14-02176 [pii] AID - 10.3390/biomedicines14102176 [doi] PST - epublish SO - Biomedicines. 2026 Sep 25;14(10):2176. doi: 10.3390/biomedicines14102176. PMID- 42939981 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 25 TI - Joint Dynamic Trajectories of CEA and CA19-9 After Gastric Cancer Surgery: Prognostic Associations and a 12-Month Landmark Analysis. LID - 10.3390/biomedicines14102175 [doi] LID - 2175 AB - Background: Static single-time-point assessment of carcinoembryonic antigen and carbohydrate antigen 19-9 cannot capture postoperative temporal patterns. We evaluated whether their joint longitudinal trajectories were associated with survival after curative gastrectomy while explicitly addressing the time-dependent bias created when trajectory groups are defined using measurements obtained after surgery. Methods: We analyzed 208 patients with serial carcinoembryonic antigen and carbohydrate antigen 19-9 measurements. Five-time-point trajectories were retained as a descriptive and exploratory analysis. Primary prognostic inference used a 12-month landmark analysis restricted to patients alive and recurrence-free at 12 months with at least three observed paired measurements from the preoperative, 1-month, 6-month, and 12-month time points. Trajectories were re-derived using only pre-landmark information. Overall survival was defined by all-cause mortality. In leakage-controlled fivefold cross-validation, imputation, transformation, cluster selection, trajectory assignment, and model fitting were performed within each training fold. Results: The 12-month landmark cohort included 164 patients and yielded four joint trajectory groups: G1, n = 60; G2, n = 41; G3, n = 32; and G4, n = 31. After adjustment for age, sex, TNM stage, differentiation, tumor site, postoperative chemotherapy, and baseline log-transformed carcinoembryonic antigen and carbohydrate antigen 19-9, G4 was associated with higher point estimates for overall survival and disease-free survival but did not reach statistical significance, with hazard ratios of 1.81 and 1.90, respectively. Adding the landmark trajectory classification increased the apparent C-index from 0.689 to 0.710 for overall survival and from 0.711 to 0.723 for disease-free survival, while likelihood-ratio tests for incremental model fit were not significant. In leakage-controlled cross-validation, the Cox model with CTS4 achieved an out-of-fold C-index of 0.589 and a 24-month post-landmark AUC of 0.635, compared with 0.614 and 0.643 for logistic regression using the same inputs. The original five-time-point surgery-indexed analysis showed stronger associations but was retained only as exploratory because the exposure incorporated future follow-up information. Conclusions: Joint carcinoembryonic antigen and carbohydrate antigen 19-9 trajectories may contain prognostic information, but the association was materially attenuated after correction for exposure timing. These findings should therefore be considered exploratory and hypothesis-generating until prospective external validation is available. FAU - Zeng, Yicong AU - Zeng Y AD - Second Department of Gastrointestinal Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China. AD - National Clinical Key Specialty (General Surgery), Sichuan Clinical Research Center for Digestive Diseases, Sichuan Branch of National Clinical Research Center for Digestive Diseases, Nanchong 637000, China. FAU - Deng, Tong AU - Deng T AD - Second Department of Gastrointestinal Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China. AD - National Clinical Key Specialty (General Surgery), Sichuan Clinical Research Center for Digestive Diseases, Sichuan Branch of National Clinical Research Center for Digestive Diseases, Nanchong 637000, China. FAU - Du, Jiali AU - Du J AD - Second Department of Gastrointestinal Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China. AD - National Clinical Key Specialty (General Surgery), Sichuan Clinical Research Center for Digestive Diseases, Sichuan Branch of National Clinical Research Center for Digestive Diseases, Nanchong 637000, China. FAU - Tang, Shiyu AU - Tang S AUID- ORCID: 0000-0001-6280-4344 AD - Division of Gastrointestinal Surgery, Department of General Surgery, West China Hospital of Sichuan University, Chengdu 610041, China. FAU - Yu, Yueyang AU - Yu Y AD - Second Department of Gastrointestinal Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China. AD - National Clinical Key Specialty (General Surgery), Sichuan Clinical Research Center for Digestive Diseases, Sichuan Branch of National Clinical Research Center for Digestive Diseases, Nanchong 637000, China. FAU - Zhang, Xudong AU - Zhang X AD - Second Department of Gastrointestinal Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China. AD - National Clinical Key Specialty (General Surgery), Sichuan Clinical Research Center for Digestive Diseases, Sichuan Branch of National Clinical Research Center for Digestive Diseases, Nanchong 637000, China. FAU - Li, Lifa AU - Li L AD - Second Department of Gastrointestinal Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China. AD - National Clinical Key Specialty (General Surgery), Sichuan Clinical Research Center for Digestive Diseases, Sichuan Branch of National Clinical Research Center for Digestive Diseases, Nanchong 637000, China. FAU - Jiang, Mengchi AU - Jiang M AD - Second Department of Gastrointestinal Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China. AD - National Clinical Key Specialty (General Surgery), Sichuan Clinical Research Center for Digestive Diseases, Sichuan Branch of National Clinical Research Center for Digestive Diseases, Nanchong 637000, China. FAU - Tang, Yumin AU - Tang Y AD - Second Department of Gastrointestinal Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China. AD - National Clinical Key Specialty (General Surgery), Sichuan Clinical Research Center for Digestive Diseases, Sichuan Branch of National Clinical Research Center for Digestive Diseases, Nanchong 637000, China. FAU - Zhou, Tong AU - Zhou T AD - Second Department of Gastrointestinal Surgery, The Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China. AD - National Clinical Key Specialty (General Surgery), Sichuan Clinical Research Center for Digestive Diseases, Sichuan Branch of National Clinical Research Center for Digestive Diseases, Nanchong 637000, China. LA - eng PT - Journal Article DEP - 20260925 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701777 OTO - NOTNLM OT - CA19-9 OT - carcinoembryonic antigen OT - gastric cancer OT - landmark analysis OT - machine learning OT - survival analysis OT - trajectory analysis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/25 CRDT- 2026/10/27 10:35 PHST- 2026/08/30 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - biomedicines14102175 [pii] AID - biomedicines-14-02175 [pii] AID - 10.3390/biomedicines14102175 [doi] PST - epublish SO - Biomedicines. 2026 Sep 25;14(10):2175. doi: 10.3390/biomedicines14102175. PMID- 42939978 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 25 TI - Safety and Preliminary Clinical Outcomes of Integrating Engineered Synthetic Lipid Vesicles (Biosomes) in a Skin Booster Protocol: A Prospective Observational Study. LID - 10.3390/biomedicines14102172 [doi] LID - 2172 AB - Background: Engineered synthetic lipid vesicles with biomimetic architecture have emerged as promising adjunctive agents in aesthetic medicine. Nevertheless, objective evidence evaluating their integration within standardized, minimally invasive skin booster protocols remains limited. The study aimed to assess the safety, tolerability, and preliminary clinical outcomes of incorporating engineered synthetic lipid vesicles (Biosomes) into a facial skin booster protocol. Methods: This prospective, single-center observational study included ten adult women aged 18-65 years. Participants received three treatment sessions at 21-day intervals, consisting of PRX PLUS application, microneedling and subsequent topical administration of engineered synthetic lipid vesicles (AnteAGE(R) Biosome Solution). Clinical assessments were conducted at baseline and 15 days after the final treatment session. Objective skin quality parameters were evaluated using the VISIA((R)) and Visioscope systems. Results: The combined protocol demonstrated a favorable tolerability profile, with minimal pain, discomfort, and burning sensation. Significant improvements were observed in texture-related parameters, pigmentation features, and redness values. Texture feature counts and scores were reduced by 20.0% and 25.8%, respectively (p 0.60). High-risk patients exhibited differences in cell cycle pathway enrichment and immune infiltration, and FN1 was positively correlated with Tregs (r = 0.60). ScRNA-seq revealed the dynamic expression of CCK, FN1, and PTGS2 in epithelial cells from paired tumor and normal samples. RT-qPCR and IHC confirmed that EGF and SPAG5 are upregulated in PCa tissue, while CCK, FN1, SELE, and PTGS2 are downregulated. Following SPAG5 knockdown, proliferation, migration, and invasion were inhibited in both cell lines. Conclusions: A reliable prognostic model screened from a stress granule-related gene set was established. Experimental data linking this model to the immune microenvironment and epithelial differentiation support the notion that SPAG5 is an oncogenic factor, suggesting that SPAG5 warrants further investigation as a potential prognostic biomarker, though prospective validation is required before clinical translation. FAU - Qin, Zitong AU - Qin Z AUID- ORCID: 0009-0008-1592-3533 AD - The First Clinical Medical College of Lanzhou University, Lanzhou 730000, China. AD - Department of Urology, The First Hospital of Lanzhou University, Lanzhou 730000, China. FAU - Wang, Ting AU - Wang T AD - Department of Urology, The Second Hospital of Lanzhou University, Lanzhou 730000, China. FAU - Liu, Runzhang AU - Liu R AD - The First Clinical Medical College of Lanzhou University, Lanzhou 730000, China. FAU - Chang, Pengcheng AU - Chang P AD - Department of Urology, The First Hospital of Lanzhou University, Lanzhou 730000, China. FAU - Li, Weiping AU - Li W AUID- ORCID: 0009-0002-1739-1565 AD - Department of Urology, The First Hospital of Lanzhou University, Lanzhou 730000, China. LA - eng GR - Grant No. lzujbky-2023-stlt01/the Fundamental Research Funds for the Central Universities/ GR - Grant No. 25JRRA1254/Gansu Province Joint Scientific Research Fund/ GR - Grant No. ldyyyn2022-3/Clinical Basic Research Program of Lanzhou University First Hospital/ PT - Journal Article DEP - 20260924 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701839 OTO - NOTNLM OT - machine learning OT - prostate cancer OT - risk model OT - single-cell RNA sequencing OT - stress granules COIS- The authors report no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:35 PHST- 2026/08/25 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - biomedicines14102165 [pii] AID - biomedicines-14-02165 [pii] AID - 10.3390/biomedicines14102165 [doi] PST - epublish SO - Biomedicines. 2026 Sep 24;14(10):2165. doi: 10.3390/biomedicines14102165. PMID- 42939966 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 24 TI - High-Intensity Focused Ultrasound Technology: Physical Principles, Biological Effects, and Clinical Applications in Facial and Neck Rejuvenation. LID - 10.3390/biomedicines14102158 [doi] LID - 2158 AB - High-Intensity Focused Ultrasound (HIFU) has emerged as a revolutionary non-invasive technology in aesthetic medicine, enabling targeted tissue remodelling and skin rejuvenation through precise thermal and mechanical effects. This narrative review provides a comprehensive examination of the physical foundations of HIFU, including acoustic wave propagation, piezoelectric energy conversion, and transducer design. It also explores its biological mechanisms, including collagen denaturation, neocollagenesis, fibroblast activation, and extracellular matrix remodelling. Clinically, HIFU has shown significant efficacy in improving skin laxity, reducing wrinkles, and enhancing facial and neck contours, with a favourable safety profile supported by multiple studies. The review also examines the limitations of conventional HIFU devices and describes the newest emerging multi-transducer platform with multi-depth cartridges and dynamic applicators. Key gaps in the literature include the scarcity of randomised controlled trials, limited data on higher Fitzpatrick skin phototypes, and the lack of standardised treatment protocols. By synthesising current evidence, this work positions HIFU as an established modality in aesthetic dermatology. A multi-transducer HIFU platform is discussed as a technically interesting emerging platform whose proposed advantages in precision, homogeneity, and comfort remain to be confirmed by independent comparative and randomised studies. FAU - Chacin, Maricarmen AU - Chacin M AUID- ORCID: 0000-0002-5208-9401 AD - Facultad de Ciencias de la Salud, Universidad Simon Bolivar, Barranquilla 080001, Colombia. FAU - Vera, Ivana AU - Vera I AUID- ORCID: 0000-0002-6596-2142 AD - Endocrine and Metabolic Diseases Research Center, School of Medicine, University of Zulia, Maracaibo 4002, Venezuela. FAU - Duran, Pablo AU - Duran P AUID- ORCID: 0000-0002-8030-1780 AD - Endocrine and Metabolic Diseases Research Center, School of Medicine, University of Zulia, Maracaibo 4002, Venezuela. FAU - Gutierrez, Valentina AU - Gutierrez V AUID- ORCID: 0009-0007-9121-2357 AD - Endocrine and Metabolic Diseases Research Center, School of Medicine, University of Zulia, Maracaibo 4002, Venezuela. FAU - Santos, Amurabit AU - Santos A AUID- ORCID: 0009-0007-0023-3305 AD - Endocrine and Metabolic Diseases Research Center, School of Medicine, University of Zulia, Maracaibo 4002, Venezuela. FAU - Contreras-Velazquez, Julio Cesar AU - Contreras-Velazquez JC AUID- ORCID: 0000-0002-5179-5400 AD - Departamento de Productividad e Innovacion, Universidad de la Costa, Barranquilla 080001, Colombia. FAU - Calero, Ana Milena Rojas AU - Calero AMR AD - Facultad de Ingenierias, Institucion Universitaria Antonio Jose Camacho, Cali 760050, Colombia. FAU - Munoz, Lady Giovanna AU - Munoz LG AD - Facultad de Ingenierias, Institucion Universitaria Antonio Jose Camacho, Cali 760050, Colombia. FAU - Lombana-Jimenez, Antony Elias AU - Lombana-Jimenez AE AUID- ORCID: 0000-0001-7290-8462 AD - Departamento de Productividad e Innovacion, Universidad de la Costa, Barranquilla 080001, Colombia. FAU - Londono Arias, Jose Alban AU - Londono Arias JA AUID- ORCID: 0000-0003-2836-5039 AD - Facultad de Ciencias Administrativas y Economicas, Tecnologico de Antioquia Institucion Universitaria, Medellin 050034, Colombia. FAU - Parias, Carlos Hernan Gonzalez AU - Parias CHG AUID- ORCID: 0000-0001-6129-8662 AD - Facultad de Ciencias Administrativas y Economicas, Tecnologico de Antioquia Institucion Universitaria, Medellin 050034, Colombia. FAU - Bermudez, Valmore AU - Bermudez V AUID- ORCID: 0000-0003-1880-8887 AD - Facultad de Ciencias de la Salud, Universidad Simon Bolivar, Barranquilla 080001, Colombia. LA - eng GR - NA/Universidad Simon Bolivar, Vicerrectoria de Investigacion, Extension e Innovacion, Internal funds for Research Strengthening/ GR - NA/Universidad Simon Bolivar, Facultad de Ciencias de la Salud,/ GR - NA/Endocrine and Metabolic Diseases Research Center, School of Medicine, University of Zulia/ GR - NA/Universidad de la Costa, Departamento de Productividad e Innovacion/ GR - NA/Facultad de Ingenierias, Institucion Universitaria Antonio Jose Camacho, Cali, Colombia; and Facultad de Ciencias Administrativas y Economicas, Tecnologico de Antioquia Institucion Universitaria/ PT - Journal Article PT - Review DEP - 20260924 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701621 OTO - NOTNLM OT - HIFU OT - aesthetic medicine OT - facial rejuvenation OT - focused ultrasound COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:35 PHST- 2026/08/13 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - biomedicines14102158 [pii] AID - biomedicines-14-02158 [pii] AID - 10.3390/biomedicines14102158 [doi] PST - epublish SO - Biomedicines. 2026 Sep 24;14(10):2158. doi: 10.3390/biomedicines14102158. PMID- 42939964 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 24 TI - Heart in Distress: How Different Shock States Converge on Cardiac Dysfunction. LID - 10.3390/biomedicines14102157 [doi] LID - 2157 AB - Shock is traditionally classified according to its primary etiology-septic, hypovolemic, obstructive, or cardiogenic-yet these syndromes frequently converge on a common feature: disturbances of cardiac performance, ventricular loading, and tissue perfusion. Despite distinct initiating mechanisms, altered preload and afterload, ventricular interdependence, vascular dysfunction, maladaptive neurohormonal responses, and, in selected states, impaired myocardial contractility may ultimately reduce effective forward flow and tissue perfusion. Echocardiography provides a rapid, noninvasive bedside tool for characterizing these evolving cardiovascular effects. It enables assessment of left and right ventricular function, cardiac filling, preload responsiveness, pulmonary and systemic venous congestion, fluid tolerance, and broader hemodynamic profiles while facilitating recognition of reversible causes such as cardiac tamponade, acute pulmonary embolism, or severe hypovolemia. This allows clinicians to move beyond static etiologic classifications toward a physiology-based assessment of shock. Beyond diagnosis, echocardiography can inform fluid resuscitation and vasoactive or inotropic therapy. Serial assessment also permits evaluation of therapeutic response and evolving hemodynamic phenotypes. This review synthesizes the mechanisms of cardiac involvement across shock syndromes and highlights echocardiography as a unifying tool for their evaluation and management. By integrating pathophysiology, bedside imaging, and hemodynamic treatment, we propose a physiology-based framework that complements traditional shock classification and may improve diagnostic precision and individualized management in critically ill patients. FAU - Zakynthinos, Georgios E AU - Zakynthinos GE AUID- ORCID: 0000-0003-4444-0967 AD - 3rd Department of Cardiology, Thoracic Diseases Hospital of Athens "Sotiria", National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Tripyla, Niki AU - Tripyla N AUID- ORCID: 0000-0003-4011-9607 AD - 3rd Department of Cardiology, Thoracic Diseases Hospital of Athens "Sotiria", National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Kokkinos, Nikolaos K AU - Kokkinos NK AD - 3rd Department of Cardiology, Thoracic Diseases Hospital of Athens "Sotiria", National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Katsarou, Ourania AU - Katsarou O AD - 3rd Department of Cardiology, Thoracic Diseases Hospital of Athens "Sotiria", National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Gialamas, Ioannis AU - Gialamas I AUID- ORCID: 0009-0003-1700-8244 AD - 3rd Department of Cardiology, Thoracic Diseases Hospital of Athens "Sotiria", National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Lysandrou, Antonios AU - Lysandrou A AD - 3rd Department of Cardiology, Thoracic Diseases Hospital of Athens "Sotiria", National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Sarantos, Savvas AU - Sarantos S AUID- ORCID: 0000-0002-5412-9382 AD - 3rd Department of Cardiology, Thoracic Diseases Hospital of Athens "Sotiria", National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Tsoukalas, Dionysios AU - Tsoukalas D AD - 3rd Department of Cardiology, Thoracic Diseases Hospital of Athens "Sotiria", National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Oikonomou, Evangelos AU - Oikonomou E AUID- ORCID: 0000-0001-8079-0599 AD - 3rd Cardiology Department, "Sotiria" General Hospital of Chest Diseases of Athens, Athina Ioanni Martinou Endowed Chair, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Siasos, Gerasimos AU - Siasos G AD - 3rd Department of Cardiology, Thoracic Diseases Hospital of Athens "Sotiria", National and Kapodistrian University of Athens, 11527 Athens, Greece. LA - eng PT - Journal Article PT - Review DEP - 20260924 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701326 OTO - NOTNLM OT - cardiac dysfunction OT - critical care OT - echocardiography OT - fluid resuscitation OT - hemodynamics OT - point-of-care ultrasound OT - right ventricular dysfunction OT - septic shock OT - shock OT - vasopressors and inotropes COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:35 PHST- 2026/07/12 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - biomedicines14102157 [pii] AID - biomedicines-14-02157 [pii] AID - 10.3390/biomedicines14102157 [doi] PST - epublish SO - Biomedicines. 2026 Sep 24;14(10):2157. doi: 10.3390/biomedicines14102157. PMID- 42939957 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 23 TI - Plasma IgG N-Glycosylation as a Novel Biomarker of Disease Severity and Prognosis in Diffuse Large B-Cell Lymphoma, Not Otherwise Specified. LID - 10.3390/biomedicines14102150 [doi] LID - 2150 AB - Background: Diffuse large B-cell lymphoma (DLBCL) is a biologically heterogeneous lymphoma with variable clinical outcomes despite established molecular and clinical prognostic models. Immunoglobulin G (IgG) N-glycosylation regulates antibody effector functions and reflects systemic immune status, but plasma IgG N-glycosylation has not previously been systematically investigated in DLBCL. We evaluated whether plasma IgG N-glycosylation is altered in DLBCL and examined its associations with molecular subtype, established prognostic indices, and clinical outcomes. Methods: Plasma samples were obtained from 54 treatment-naive patients with pathologically confirmed DLBCL, not otherwise specified (NOS), and 54 age- and sex-matched healthy controls. IgG N-glycans were analyzed using capillary gel electrophoresis with laser-induced fluorescence (CGE-LIF), resolving 27 glycan peaks that were summarized into six derived glycosylation traits: agalactosylation (G0), monogalactosylation (G1), digalactosylation (G2), sialylation (S), bisecting N-acetylglucosamine (B), and core fucosylation (CF). Associations with disease status, cell-of-origin subtype, prognostic indices (International Prognostic Index (IPI), Age-Adjusted International Prognostic Index (aaIPI), revised International Prognostic Index (R-IPI), National Comprehensive Cancer Network International Prognostic index (NCCN-IPI), Central Nervous System International Prognostic Index (CNS-IPI) and inflammation-based cumulative prognostic score system (ICPS)) progression-free survival (PFS), overall survival (OS), and relapse were assessed using multivariable regression and Cox proportional hazards models adjusted for age and sex. Multiple testing was controlled using the Benjamini-Hochberg false discovery rate. Results: Compared with healthy controls, patients with DLBCL, NOS exhibited significantly increased agalactosylation and decreased digalactosylation and sialylation, consistent with a pro-inflammatory IgG glycosylation profile. The non-germinal center B-cell-like (non-GCB) subtype showed significantly lower monogalactosylation than germinal center B-cell like (GCB) DLBCL. Across multiple established prognostic indices, increasing clinical risk was consistently associated with increased agalactosylation and reduced digalactosylation. During follow-up, 20 patients (37%) relapsed, and 21 (39%) died. Increased agalactosylation was independently associated with shorter PFS, whereas reduced sialylation predicted both inferior PFS and an increased risk of relapse. Conclusions: Plasma IgG N-glycosylation is profoundly altered in DLBCL, NOS and is associated with molecular subtype, established prognostic indices, and clinically relevant outcomes. These findings identify plasma IgG N-glycosylation as a promising minimally invasive biomarker that reflects disease biology and provides prognostic information beyond conventional clinical risk models. Given the exploratory nature and modest size of this cohort, these findings should be considered hypothesis-generating and require validation in larger prospective cohorts. FAU - Milunovic, Vibor AU - Milunovic V AUID- ORCID: 0000-0003-0069-6688 AD - Division of Hematology, Clinical Hospital Merkur, 10000 Zagreb, Croatia. FAU - Pucic-Bakovic, Maja AU - Pucic-Bakovic M AUID- ORCID: 0000-0003-0866-623X AD - Glycoscience Research Laboratory, Genos Ltd., 10000 Zagreb, Croatia. FAU - Vuckovic, Frano AU - Vuckovic F AD - Glycoscience Research Laboratory, Genos Ltd., 10000 Zagreb, Croatia. FAU - Duvnjak-Oreskovic, Ivana AU - Duvnjak-Oreskovic I AUID- ORCID: 0009-0000-4807-8913 AD - Glycoscience Research Laboratory, Genos Ltd., 10000 Zagreb, Croatia. FAU - Misura Jakobac, Karla AU - Misura Jakobac K AUID- ORCID: 0000-0001-7353-8811 AD - Division of Hematology, Clinical Hospital Merkur, 10000 Zagreb, Croatia. FAU - Kursar, Marin AU - Kursar M AUID- ORCID: 0000-0002-1558-0058 AD - Division of Hematology, Clinical Hospital Merkur, 10000 Zagreb, Croatia. FAU - Mandac Smoljanovic, Inga AU - Mandac Smoljanovic I AUID- ORCID: 0000-0001-5234-9464 AD - Division of Hematology, Clinical Hospital Merkur, 10000 Zagreb, Croatia. AD - School of Medicine in Zagreb, University of Zagreb, 10000 Zagreb, Croatia. FAU - Radic-Kristo, Delfa AU - Radic-Kristo D AD - Division of Hematology, Clinical Hospital Merkur, 10000 Zagreb, Croatia. AD - School of Medicine in Zagreb, University of Zagreb, 10000 Zagreb, Croatia. FAU - Gasparov, Slavko AU - Gasparov S AUID- ORCID: 0000-0003-3592-031X AD - School of Medicine in Zagreb, University of Zagreb, 10000 Zagreb, Croatia. AD - Clinical Department of Pathology and Cytology, Clinical Hospital Merkur, 10000 Zagreb, Croatia. LA - eng GR - Croatian Cooperative Group for Hematologic Disease (KROHEM)/ PT - Journal Article DEP - 20260923 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701574 OTO - NOTNLM OT - DLBCL OT - IgG glycosylation OT - biomarker OT - prognosis OT - prognostic indices COIS- M.P.-B., F.V., and I.D.O. are employees of Genos Ltd., a company that offers commercial service of glycomic analysis and has several patents in this field. None of the other authors have a conflict of interest to share. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/23 CRDT- 2026/10/27 10:35 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - biomedicines14102150 [pii] AID - biomedicines-14-02150 [pii] AID - 10.3390/biomedicines14102150 [doi] PST - epublish SO - Biomedicines. 2026 Sep 23;14(10):2150. doi: 10.3390/biomedicines14102150. PMID- 42939955 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 23 TI - Stimulus-Induced Histone H3 Citrullination and Neutrophil Extracellular Trap Formation Are Impaired in Patients with Proliferative Diabetic Retinopathy. LID - 10.3390/biomedicines14102148 [doi] LID - 2148 AB - Background: Proliferative diabetic retinopathy (PDR) is the most advanced stage of diabetic retinopathy (DR). Although spontaneous release of Neutrophil Extracellular Traps (NETs) has been reported in DR patients, the capacity of neutrophils from PDR patients to undergo inducible NET formation remains unexplored. This study aimed to evaluate inducible NET release and the underlying regulatory mechanisms in PDR. Methods: We performed a case-control study including 9 nondiabetic controls, 17 patients with type 2 diabetes (T2D) without DR, and 15 patients with PDR. Peripheral neutrophils were isolated and stimulated with ionomycin to assess NET release and histone H3 citrullination (H3Cit). Peptidylarginine deiminase 4 (PADI4)promoter methylation and mRNA expression were analyzed, together with the surface expression of the activation markers CD11b and CD66b. An ex vivo high-glucose model was used to examine the effect of persistent hyperglycemia on NET induction. Results: Compared with those from controls and T2D patients, neutrophils from PDR patients exhibited significantly reduced inducible NET release and impaired H3Cit. The NET index (stimulated/nonstimulated ratio) was markedly lower in the PDR group. Despite increased PADI4 mRNA expression in PDR neutrophils, promoter methylation, as well as that of cell activation markers, remained unchanged. Notably, persistent hyperglycemia in healthy neutrophils reproduced the impaired NET release and reduced H3Cit observed in PDR. Conclusions: Neutrophils from PDR patients display selective defects in inducible NET release and histone citrullination, which are likely driven by chronic hyperglycemia and metabolic stress rather than epigenetic silencing of PADI4. These findings are consistent with impaired inducible neutrophil responsiveness in PDR and suggest that altered regulation of PAD4-dependent histone citrullination may contribute to this phenotype, warranting further mechanistic investigation. FAU - Buentello-Volante, Beatriz AU - Buentello-Volante B AUID- ORCID: 0000-0003-4529-990X AD - Cell and Tissue Department, Research Unit, Institute of Ophthalmology Conde de Valenciana, Mexico City 06800, Mexico. FAU - Magana-Guerrero, Fatima Sofia AU - Magana-Guerrero FS AUID- ORCID: 0000-0002-1535-9898 AD - Cell and Tissue Department, Research Unit, Institute of Ophthalmology Conde de Valenciana, Mexico City 06800, Mexico. FAU - Magana-Guerrero, Norma Angelica AU - Magana-Guerrero NA AD - Cell and Tissue Department, Research Unit, Institute of Ophthalmology Conde de Valenciana, Mexico City 06800, Mexico. AD - Department of Biochemistry, Faculty of Medicine, Universidad Nacional Autonoma de Mexico, Av. Universidad 3000, Mexico City 04510, Mexico. FAU - Rodriguez-Loaiza, Jose Luis AU - Rodriguez-Loaiza JL AUID- ORCID: 0009-0005-7122-4591 AD - Department of Retina, Institute of Ophthalmology Conde de Valenciana, Mexico City 06800, Mexico. FAU - Vivanco-Rojas, Oscar AU - Vivanco-Rojas O AUID- ORCID: 0000-0002-7745-7581 AD - Cell and Tissue Department, Research Unit, Institute of Ophthalmology Conde de Valenciana, Mexico City 06800, Mexico. AD - Department of Biochemistry, Faculty of Medicine, Universidad Nacional Autonoma de Mexico, Av. Universidad 3000, Mexico City 04510, Mexico. FAU - Dominguez-Lopez, Alfredo AU - Dominguez-Lopez A AD - Cell and Tissue Department, Research Unit, Institute of Ophthalmology Conde de Valenciana, Mexico City 06800, Mexico. AD - Department of Biochemistry, Faculty of Medicine, Universidad Nacional Autonoma de Mexico, Av. Universidad 3000, Mexico City 04510, Mexico. FAU - Garfias, Yonathan AU - Garfias Y AUID- ORCID: 0000-0003-3753-1035 AD - Cell and Tissue Department, Research Unit, Institute of Ophthalmology Conde de Valenciana, Mexico City 06800, Mexico. AD - Department of Biochemistry, Faculty of Medicine, Universidad Nacional Autonoma de Mexico, Av. Universidad 3000, Mexico City 04510, Mexico. LA - eng GR - 159/2023/Secretaria de Educacion, Ciencia, Tecnologia e Innovacion de la Ciudad de Mexico (SECTEI)/ GR - 138/2025/Secretaria de Educacion, Ciencia, Tecnologia e Innovacion de la Ciudad de Mexico (SECTEI)/ GR - PAPIIT-DGAPA-UNAM IN210224/Universidad Nacional Autonoma de Mexico/ GR - UNAM-DGAPA-PAPIIT- IA208226/Universidad Nacional Autonoma de Mexico/ GR - Institute of Ophthalmology/ PT - Journal Article DEP - 20260923 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701284 OTO - NOTNLM OT - PADI4 OT - histone H3 citrullination OT - immune dysfunction OT - neutrophil extracellular traps (NETs) OT - proliferative diabetic retinopathy COIS- The authors declare that they have no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/23 CRDT- 2026/10/27 10:35 PHST- 2026/07/15 00:00 [received] PHST- 2026/09/15 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - biomedicines14102148 [pii] AID - biomedicines-14-02148 [pii] AID - 10.3390/biomedicines14102148 [doi] PST - epublish SO - Biomedicines. 2026 Sep 23;14(10):2148. doi: 10.3390/biomedicines14102148. PMID- 42939952 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 23 TI - Toward Endotype-Based Bronchopulmonary Dysplasia: Evolving Definitions and the Role of Translational Models. LID - 10.3390/biomedicines14102145 [doi] LID - 2145 AB - Bronchopulmonary dysplasia (BPD) remains one of the most frequent and clinically relevant chronic respiratory complications of extreme prematurity. Although successive definitions have improved disease classification, most are still based on oxygen requirement and respiratory support assessed at 36 weeks postmenstrual age (PMA), an approach that fails to capture the biological heterogeneity, dynamic clinical trajectories and long-term consequences of the disease. This narrative review analyzes the evolution of BPD definitions, from early time-based criteria to contemporary classifications based on severity and respiratory support, highlighting their strengths and limitations for predicting outcomes and translational research. We argue for the need to move beyond BPD as a single clinical entity toward a framework based on phenotypes and endotypes, including the parenchymal, airway, vascular, inflammatory, and mechanical components of the disease. Although non-animal alternatives are rapidly evolving, animal models remain the primary tools for analyzing specific developmental windows, injurious exposures, and molecular pathways involved in impaired alveolarization, abnormal vascular growth, inflammation, and ventilator-induced lung injury. We propose an endotype-based approach to guide the selection of preclinical models, supported by standardized injury protocols, longitudinal multimodal measurements, and the integration of emerging technologies such as organoids, lung-on-a-chip platforms, multi-omics, and predictive modeling. Redefining BPD through a translational and mechanism-based framework may improve risk stratification and support precision neonatal therapies. FAU - Rodriguez-Rey, Marianela AU - Rodriguez-Rey M AUID- ORCID: 0000-0002-8959-2035 AD - Departamento de Neonatologia, Hospital de Clinicas, Facultad de Medicina, Universidad de la Republica, Montevideo CP 11600, Uruguay. AD - Centro de Investigaciones Biomedicas (CEINBIO), Universidad de la Republica, Montevideo CP 11600, Uruguay. FAU - Valez, Valeria AU - Valez V AUID- ORCID: 0000-0001-5937-0215 AD - Centro de Investigaciones Biomedicas (CEINBIO), Universidad de la Republica, Montevideo CP 11600, Uruguay. AD - Departamento de Biologia Odontologica, Subunidad Bioquimica y Biofisica Facultad de Odontologia, Universidad de la Republica, Montevideo CP 11600, Uruguay. FAU - Caballero, Mauricio T AU - Caballero MT AUID- ORCID: 0000-0002-4218-5942 AD - Centro Infant de Medicina Traslacional (CIMeT), Escuela de Bio y Nanotecnologias (EByN), Universidad Nacional de San Martin (UNSAM), General San Martin CP 1650, Provincia de Buenos Aires, Argentina. AD - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires ZIP 1425, Argentina. FAU - Alvarez-Paggi, Damian AU - Alvarez-Paggi D AUID- ORCID: 0000-0002-1507-9685 AD - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Buenos Aires ZIP 1425, Argentina. AD - Centro de Rediseno e Ingenieria de Proteinas (CRIP), Escuela de Bio y Nanotecnologias (EByN), Universidad Nacional de San Martin (UNSAM), General San Martin CP 1650, Provincia de Buenos Aires, Argentina. LA - eng PT - Journal Article PT - Review DEP - 20260923 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701936 OTO - NOTNLM OT - animal models OT - bronchopulmonary dysplasia OT - hyperoxia OT - hyperoxia-induced lung injury OT - premature infant OT - translational research COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/23 CRDT- 2026/10/27 10:35 PHST- 2026/06/16 00:00 [received] PHST- 2026/08/29 00:00 [revised] PHST- 2026/09/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - biomedicines14102145 [pii] AID - biomedicines-14-02145 [pii] AID - 10.3390/biomedicines14102145 [doi] PST - epublish SO - Biomedicines. 2026 Sep 23;14(10):2145. doi: 10.3390/biomedicines14102145. PMID- 42939949 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 22 TI - Plant-Derived Dipeptidyl Peptidase-4 Inhibitors in Cardiometabolic Disorders: A Critical Review of Natural Scaffolds and Experimental Evidence. LID - 10.3390/biomedicines14102141 [doi] LID - 2141 AB - Cardiometabolic disorders, including obesity, insulin resistance and type 2 diabetes mellitus, remain major biomedical problems closely linked to vascular complications. Dipeptidyl peptidase-4 (DPP-4) is a clinically validated therapeutic target because inhibition of this enzyme prolongs the activity of incretin hormones and supports glucose-dependent insulin secretion. This review compiles and critically evaluates plant-derived DPP-4 inhibitors, with emphasis on chemical class, enzyme source, quantitative activity, assay interpretability, and the translational relevance of reported findings. The available literature indicates that flavonoids and polyphenolic compounds dominate the field, although assay heterogeneity, medium effects, potential aggregation, and insufficient selectivity data limit interpretation. Among natural scaffolds, chalcones and aurones, including lanceoletin, leptosidin, xanthoangelol, chalconaringenin 2'-O-beta-D-glucopyranoside, and aureusidin 6-O-beta-D-glucopyranoside, represent comparatively well-supported lead-like scaffolds that warrant further standardized evaluation. The evidence does not support using plant-derived DPP-4 inhibitors as substitutes for approved antidiabetic drugs. Their greatest value lies in expanding chemical space and providing lead-like scaffolds for standardized potency, selectivity, kinetic and preclinical validation. FAU - Zhapar, Kuanysh AU - Zhapar K AUID- ORCID: 0000-0002-9007-9730 AD - Centre for Life Sciences, National Laboratory Astana, Nazarbayev University, Kabanbay Batyr Avenue 53, Astana 010000, Kazakhstan. FAU - Samatkyzy, Diana AU - Samatkyzy D AD - Centre for Life Sciences, National Laboratory Astana, Nazarbayev University, Kabanbay Batyr Avenue 53, Astana 010000, Kazakhstan. FAU - Valishayeva, Diana AU - Valishayeva D AUID- ORCID: 0009-0009-8452-8307 AD - Centre for Life Sciences, National Laboratory Astana, Nazarbayev University, Kabanbay Batyr Avenue 53, Astana 010000, Kazakhstan. FAU - Rakhimova, Saule AU - Rakhimova S AUID- ORCID: 0000-0002-8245-2400 AD - Centre for Life Sciences, National Laboratory Astana, Nazarbayev University, Kabanbay Batyr Avenue 53, Astana 010000, Kazakhstan. FAU - Sarbassov, Dos AU - Sarbassov D AUID- ORCID: 0000-0002-6848-1133 AD - Centre for Life Sciences, National Laboratory Astana, Nazarbayev University, Kabanbay Batyr Avenue 53, Astana 010000, Kazakhstan. AD - Department of Biological and Biomedical Sciences, School of Medicine, Nazarbayev University, Kabanbay Batyr Avenue 53, Astana 010000, Kazakhstan. FAU - Akilzhanova, Ainur AU - Akilzhanova A AUID- ORCID: 0000-0001-6161-8355 AD - Centre for Life Sciences, National Laboratory Astana, Nazarbayev University, Kabanbay Batyr Avenue 53, Astana 010000, Kazakhstan. LA - eng GR - BR24993023, AP23490249, BR24992841/Science Committee of the Ministry of Science and Higher Education of the Republic of Kazakhstan/ PT - Journal Article PT - Review DEP - 20260922 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701426 OTO - NOTNLM OT - DPP-4 OT - aurones OT - cardiometabolic disorders OT - chalcones OT - dipeptidyl peptidase-4 OT - flavonoids OT - natural products OT - plant-derived inhibitors OT - type 2 diabetes mellitus COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:35 PHST- 2026/08/19 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - biomedicines14102141 [pii] AID - biomedicines-14-02141 [pii] AID - 10.3390/biomedicines14102141 [doi] PST - epublish SO - Biomedicines. 2026 Sep 22;14(10):2141. doi: 10.3390/biomedicines14102141. PMID- 42939946 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 22 TI - Gallstone Status Classification Using Adaptive Performance-Diversity Hybrid Ensemble with Pareto-Based Multi-Objective Selection (APDHE-Pareto). LID - 10.3390/biomedicines14102139 [doi] LID - 2139 AB - Background/Objectives: Machine-learning models may support gallstone status classification using heterogeneous clinical and body-composition variables; however, ensemble methods often rely on predefined model combinations or arbitrarily weighted performance criteria. This study proposes the Adaptive Performance-Diversity Hybrid Ensemble with Pareto-Based Multi-Objective Selection (APDHE-Pareto), a leakage-controlled multi-objective ensemble framework that integrates complementary performance measures, prediction diversity, and cross-validation stability. Methods: An open-access dataset comprising 319 observations and 38 predictors was divided into a development set (n = 255) and a held-out internal test set (n = 64). Candidate classifiers were evaluated using Pareto non-dominated sorting across ROC-AUC, balanced accuracy, F1-score, and Matthews correlation coefficient. Hyperparameter optimization, probability calibration, ensemble construction, and threshold optimization were performed exclusively within the development process. Performance was assessed using repeated 10-fold nested stratified cross-validation (100 outer evaluations) and a one-time evaluation on the held-out internal test set. Results: Among the primary model comparisons, APDHE-Pareto achieved the highest mean ROC-AUC during repeated nested cross-validation (0.859). On the held-out internal test set, it achieved the highest ROC-AUC (0.910), balanced accuracy (0.828), F1-score (0.814), Matthews correlation coefficient (0.664), and the lowest Brier score (0.131) among the evaluated models. Formal paired comparison with the prespecified Extra Trees baseline showed no statistically significant difference in classification errors or ROC-AUC. Conclusions: APDHE-Pareto provides a systematic and leakage-controlled framework for multi-objective ensemble selection while achieving competitive predictive performance. Independent external validation is required to confirm its generalizability and potential clinical utility. FAU - Yapici, Irem Senyer AU - Yapici IS AUID- ORCID: 0000-0003-0655-340X AD - Department of Computer Engineering, Zonguldak Bulent Ecevit University, Zonguldak 67100, Turkiye. LA - eng PT - Journal Article DEP - 20260922 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701348 OTO - NOTNLM OT - Pareto optimization OT - ensemble learning OT - explainable artificial intelligence OT - gallstone disease OT - machine learning OT - model stability OT - multi-objective optimization OT - nested cross-validation OT - prediction diversity COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:35 PHST- 2026/08/25 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - biomedicines14102139 [pii] AID - biomedicines-14-02139 [pii] AID - 10.3390/biomedicines14102139 [doi] PST - epublish SO - Biomedicines. 2026 Sep 22;14(10):2139. doi: 10.3390/biomedicines14102139. PMID- 42939944 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 22 TI - Gilteritinib Suppresses Hepatocellular Carcinoma Cells in Association with Reduced Calpain-1 Expression and Inhibition of STAT3/AKT Signaling. LID - 10.3390/biomedicines14102137 [doi] LID - 2137 AB - Background/Objectives: Hepatocellular carcinoma (HCC) is a leading cause of cancer-associated mortality worldwide, and current targeted therapeutic options remain inadequate. Gilteritinib, a clinically approved tyrosine kinase inhibitor, has shown antitumor activity in hematologic malignancies; however, its therapeutic role and underlying mechanisms in HCC remain unclear. Methods: The effects of gilteritinib on HCC were evaluated in Hepa1-6 and Huh-7 cells. Cell proliferation, migration, invasion, apoptosis, and cell cycle distribution were assessed by CCK-8, wound healing, Transwell, and flow cytometry assays. Protein expression and total calpain activity were assessed by Western blotting and enzymatic activity assays. Surface plasmon resonance (SPR) analysis was used to examine the cellular association of gilteritinib with HCC cells. Antitumor efficacy and safety were further evaluated in a Hepa1-6 allograft mouse model. Results: Gilteritinib significantly inhibited the proliferation, migration, and invasion of HCC cells and induced G2/M-phase arrest and apoptosis. Gilteritinib treatment was associated with reduced calpain-1 expression and total calpain activity. These changes were accompanied by decreased phosphorylation of STAT3 and AKT, downregulation of Bcl-2, and upregulation of Bax. In vivo, gilteritinib markedly suppressed tumor growth in Hepa1-6 tumor-bearing mice. Conclusions: Collectively, gilteritinib exerts antitumor effects against HCC both in vitro and in vivo, and these effects are closely associated with reduced calpain-1 expression and inhibition of STAT3/AKT signaling. These findings suggest that gilteritinib has therapeutic potential in HCC and highlight calpain-1 as a candidate downstream correlate worthy of further mechanistic study. FAU - Chen, Sijing AU - Chen S AD - School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China. AD - Gansu Province Key Laboratory of Environment Oncology, Lanzhou University Second Hospital, Lanzhou 730030, China. FAU - Zhao, Yunyang AU - Zhao Y AD - School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China. AD - Gansu Province Key Laboratory of Environment Oncology, Lanzhou University Second Hospital, Lanzhou 730030, China. FAU - Pan, Yunqi AU - Pan Y AD - Department of Cardiovascular Disease, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou 730000, China. FAU - Zhao, Zhihui AU - Zhao Z AD - School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China. FAU - Ma, Zhonghua AU - Ma Z AD - Department of Urology, Minqin County People's Hospital, Minqin 733300, China. FAU - Wei, Yujie AU - Wei Y AD - Department of Cardiovascular Disease, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou 730000, China. FAU - Liu, Ting AU - Liu T AD - School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China. AD - Gansu Province Key Laboratory of Environment Oncology, Lanzhou University Second Hospital, Lanzhou 730030, China. FAU - Shuai, Limei AU - Shuai L AD - School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China. AD - Gansu Province Key Laboratory of Environment Oncology, Lanzhou University Second Hospital, Lanzhou 730030, China. FAU - Gu, Wenjiao AU - Gu W AD - Department of Cardiovascular Disease, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou 730000, China. FAU - Li, Shuangyi AU - Li S AD - Department of Cardiovascular Disease, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou 730000, China. FAU - Shao, Junjie AU - Shao J AD - Department of Cardiovascular Disease, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou 730000, China. FAU - Jiang, Ying AU - Jiang Y AD - Department of Cardiovascular Disease, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou 730000, China. FAU - Maimaitituerxun, Ailifeire AU - Maimaitituerxun A AD - Department of Cardiovascular Disease, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou 730000, China. FAU - Wang, Xinghuai AU - Wang X AD - Department of Cardiovascular Disease, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou 730000, China. FAU - Zhou, Xiaochun AU - Zhou X AD - Department of Nephrology, Lanzhou University Second Hospital, Lanzhou 730030, China. FAU - Tang, Futian AU - Tang F AD - Gansu Province Key Laboratory of Environment Oncology, Lanzhou University Second Hospital, Lanzhou 730030, China. AD - Department of Cardiovascular Disease, The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou 730000, China. LA - eng GR - lzujbky-2022-sp08/Lanzhou University/ GR - 20250050159/Lanzhou University/ GR - lzuyxcx-2022-154；lzuyxcx-2022-141/Lanzhou University/ GR - 81870329；81960673/National Natural Science Foundation of China/ GR - 21JR1RA135；23JRRA1001/Natural Science Foundation of Gansu Province/ GR - WW23B02SF074/Scienceand Technology Program of Wuwei Municipality/ PT - Journal Article DEP - 20260922 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701972 OTO - NOTNLM OT - STAT3/AKT signaling pathway OT - apoptosis OT - calpain-1 OT - gilteritinib OT - hepatocellular carcinoma COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:35 PHST- 2026/08/13 00:00 [received] PHST- 2026/09/12 00:00 [revised] PHST- 2026/09/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - biomedicines14102137 [pii] AID - biomedicines-14-02137 [pii] AID - 10.3390/biomedicines14102137 [doi] PST - epublish SO - Biomedicines. 2026 Sep 22;14(10):2137. doi: 10.3390/biomedicines14102137. PMID- 42939943 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2227-9059 (Print) IS - 2227-9059 (Electronic) IS - 2227-9059 (Linking) VI - 14 IP - 10 DP - 2026 Sep 22 TI - Genetically Predicted Circulating Lipid Traits and Risk of Primary Open-Angle Glaucoma: A Mendelian Randomization Study. LID - 10.3390/biomedicines14102136 [doi] LID - 2136 AB - Background: Observational studies examining the relationship between serum lipids and primary open-angle glaucoma (POAG) have yielded inconsistent findings, limiting causal inference. Mendelian randomization (MR) offers a robust approach to evaluate causality by leveraging genetic variants as proxies for modifiable exposures, thereby minimizing confounding and reverse causation. Methods: A two-sample MR study was conducted using GWAS summary statistics for 179 circulating lipid traits (GCST90277238-GCST90277416) as exposures and POAG cases from the FinnGen consortium (Release R12, endpoint H7_GLAUCOMA_POAG) as the outcome. Genetic variants were selected as instrumental variables at p < 1 x 10(-5) and clumped for linkage disequilibrium (R(2) < 0.001). The inverse variance weighted (IVW) method was the primary analysis. Multiple testing was addressed using both Bonferroni and false discovery rate (FDR) correction. Instrument strength was assessed via F-statistics for all 179 traits. Sensitivity analyses included MR-Egger regression, weighted median, weighted mode, MR-PRESSO, Cochran's Q, and leave-one-out analysis. Results: Of 179 lipid traits analyzed, one, phosphatidylcholine (16:0/18:1), demonstrated a statistically significant protective association with POAG after FDR correction (beta = -0.22, SE = 0.06, OR = 0.80, 95% CI: 0.71-0.90; p = 1.77 x 10(-4), FDR q = 0.032). No other lipid trait survived multiple testing correction. Sensitivity analyses showed no evidence of horizontal pleiotropy or significant heterogeneity. Conclusions: This study provides genetic evidence for a protective association between phosphatidylcholine (16:0/18:1) and POAG risk; however, conditional analyses indicate this association is not fully independent of genetically correlated lipid species, and species-level specificity remains to be formally established. Phosphatidylcholine (16:0/18:1) warrants further investigation as a potential causal factor in glaucoma pathogenesis. FAU - El Ashry, Ahmed AU - El Ashry A AUID- ORCID: 0009-0000-2645-3989 AD - Medical School, University of Bristol, Bristol BS8 1UD, UK. FAU - El Ashry, Mahmoud AU - El Ashry M AUID- ORCID: 0009-0007-5253-6906 AD - Medical School, University of Bristol, Bristol BS8 1UD, UK. FAU - Saeed, Hajirah N AU - Saeed HN AUID- ORCID: 0000-0002-7385-6534 AD - Department of Ophthalmology and Visual Sciences, University of Illinois Chicago, Chicago, IL 60612, USA. FAU - Elhusseiny, Abdelrahman M AU - Elhusseiny AM AUID- ORCID: 0000-0003-1412-375X AD - Department of Ophthalmology, Bascom Palmer Eye Institute, Miami, FL 33136, USA. AD - Department of Ophthalmology, Jones Eye Institute, Little Rock, AR 72205, USA. AD - Department of Ophthalmology, Boston Children's Hospital, Boston, MA 02115, USA. LA - eng PT - Journal Article DEP - 20260922 PL - Switzerland TA - Biomedicines JT - Biomedicines JID - 101691304 PMC - PMC13701849 OTO - NOTNLM OT - genetic lipid indicators OT - genetic risk factors OT - glaucoma OT - lipid OT - lipidomics OT - mendelian OT - open angle OT - phosphatidylcholine OT - traits COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:35 PHST- 2026/08/14 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:35 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - biomedicines14102136 [pii] AID - biomedicines-14-02136 [pii] AID - 10.3390/biomedicines14102136 [doi] PST - epublish SO - Biomedicines. 2026 Sep 22;14(10):2136. doi: 10.3390/biomedicines14102136. PMID- 42939941 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3921 (Print) IS - 2076-3921 (Electronic) IS - 2076-3921 (Linking) VI - 15 IP - 10 DP - 2026 Oct 21 TI - Oxidative Stress and Alveolar-Capillary Barrier Failure in Sepsis- and Severe Acute Pancreatitis-Associated ARDS. LID - 10.3390/antiox15101368 [doi] LID - 1368 AB - Acute respiratory distress syndrome (ARDS) is the final common phenotype of diverse insults, but the route to alveolar-capillary barrier failure matters. Sepsis and severe acute pancreatitis provide an informative contrast: sepsis begins with infection and pathogen-associated molecular patterns, whereas pancreatitis is initially driven by sterile acinar-cell injury, necrosis and damage-associated molecular patterns, before infected necrosis can superimpose sepsis biology. These differences are clinically relevant since they determine whether redox-related signals must be interpreted in the context of pathogen burden and antimicrobial host defense or of initially sterile necrosis with subsequent risk of infected necrosis. In this review, we discuss how these distinct pathophysiological contexts engage NADPH oxidase (NOX)-derived oxidants, mitochondrial reactive oxygen species, reactive nitrogen species (RNS), lipid peroxidation, regulated cell death and impaired antioxidant defenses. We focus on the pulmonary endothelium and epithelium as compartments in which inflammatory and redox signals converge to disrupt barrier integrity, impair alveolar fluid clearance and promote immunothrombosis. We also discuss coronavirus disease 2019 (COVID-19)-associated ARDS as a viral comparator, the analytical limitations of redox biomarkers, and the value of serial, compartment-aware measurements. The central message is that oxidative stress is not a single systemic deficiency that can be corrected by generic antioxidants. Its biological and therapeutic significance depends on oxidant source, cellular compartment, microbial burden and disease phase. However, evidence for many specific redox pathways remains predominantly preclinical, and their clinical relevance requires further validation. At present, no validated redox-directed treatment-selection strategy exists; future approaches will therefore require validated target-engagement markers and phenotype- and time-specific trial designs. FAU - Rusch, Sophia AU - Rusch S AD - Department of Internal Medicine I, Gastroenterology, Hepatology, Endocrinology, Rheumatology, Immunology, and Infectious Diseases, University Hospital Regensburg, 93053 Regensburg, Germany. FAU - Mester, Patricia AU - Mester P AD - Department of Internal Medicine I, Gastroenterology, Hepatology, Endocrinology, Rheumatology, Immunology, and Infectious Diseases, University Hospital Regensburg, 93053 Regensburg, Germany. FAU - Moller, Fabian AU - Moller F AD - Department of Internal Medicine I, Gastroenterology, Hepatology, Endocrinology, Rheumatology, Immunology, and Infectious Diseases, University Hospital Regensburg, 93053 Regensburg, Germany. FAU - Muller, Martina AU - Muller M AUID- ORCID: 0000-0002-8520-4568 AD - Department of Internal Medicine I, Gastroenterology, Hepatology, Endocrinology, Rheumatology, Immunology, and Infectious Diseases, University Hospital Regensburg, 93053 Regensburg, Germany. FAU - Gulow, Karsten AU - Gulow K AUID- ORCID: 0009-0006-2897-3447 AD - Department of Internal Medicine I, Gastroenterology, Hepatology, Endocrinology, Rheumatology, Immunology, and Infectious Diseases, University Hospital Regensburg, 93053 Regensburg, Germany. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Antioxidants (Basel) JT - Antioxidants (Basel, Switzerland) JID - 101668981 PMC - PMC13701611 OTO - NOTNLM OT - acute respiratory distress syndrome (ARDS) OT - mitochondrial dysfunction OT - oxidative stress OT - reactive oxygen species (ROS) OT - sepsis OT - severe acute pancreatitis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:34 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - antiox15101368 [pii] AID - antioxidants-15-01368 [pii] AID - 10.3390/antiox15101368 [doi] PST - epublish SO - Antioxidants (Basel). 2026 Oct 21;15(10):1368. doi: 10.3390/antiox15101368. PMID- 42939930 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3921 (Print) IS - 2076-3921 (Electronic) IS - 2076-3921 (Linking) VI - 15 IP - 10 DP - 2026 Oct 20 TI - Effects of Ellagic Acid on Gut Microbiota and Tryptophan Metabolism in Paraquat-Challenged Piglets Subjected to Ampicillin-Induced Microbiota Perturbation. LID - 10.3390/antiox15101356 [doi] LID - 1356 AB - Oxidative stress (OS) is closely associated with intestinal dysfunction, gut microbial dysbiosis, and metabolic disturbances in weaned piglets. Ellagic acid (EA) is a dietary polyphenol with antioxidant properties. This study investigated the effects of EA supplementation and ampicillin (AMP)-induced microbial perturbation on OS, gut microbiota, and tryptophan (Trp) metabolism in paraquat (PQ)-challenged piglets. Twenty-four weaned piglets were assigned to four treatments in a 2 x 2 factorial design (+/-EA, +/-AMP), followed by a PQ challenge. Neither EA nor AMP affected growth performance or most serum biochemical parameters. EA significantly decreased malondialdehyde (MDA) in AMP-treated piglets. AMP altered ileal morphology and reshaped the microbial community in both the ileum and colon. Moreover, EA and AMP differentially affected Trp metabolism in serum and intestinal segments; AMP altered kynurenine-pathway and microbiota-associated indole metabolites, with significant EA x AMP interactions for some metabolites. Spearman analysis further revealed distinct associations between gut microbiota and Trp metabolites along the intestinal tract. Collectively, AMP-induced microbial perturbation was accompanied by substantial Trp-metabolism alterations and distinct microbiota-Trp associations, whereas EA modulated MDA levels and selected microbial and metabolic responses under AMP treatment. These observations suggest that EA effects under OS may be associated with gut microbiota-Trp metabolism axis modulation, although causality remains to be established. FAU - Deng, Yuankun AU - Deng Y AD - Xiangya School of Nursing, Central South University, Changsha 410013, China. AD - Hunan Provincial Key Laboratory for the Products Quality Regulation of Livestock and Poultry, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China. FAU - Wang, Chengming AU - Wang C AUID- ORCID: 0009-0007-8391-4551 AD - Hunan Provincial Key Laboratory for the Products Quality Regulation of Livestock and Poultry, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China. AD - Department of Animal Science, Shandong Agricultural University, Taian 271017, China. FAU - Meng, Yunlong AU - Meng Y AD - Hunan Provincial Key Laboratory for the Products Quality Regulation of Livestock and Poultry, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China. FAU - Yin, Yulong AU - Yin Y AD - Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China. FAU - Wang, Jing AU - Wang J AD - Hunan Provincial Key Laboratory for the Products Quality Regulation of Livestock and Poultry, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China. FAU - Ye, Qiuping AU - Ye Q AD - Xiangya School of Nursing, Central South University, Changsha 410013, China. AD - School of Nursing, Guiyang Kangyang University, Guiyang 550081, China. LA - eng GR - 32573255/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Antioxidants (Basel) JT - Antioxidants (Basel, Switzerland) JID - 101668981 PMC - PMC13701896 OTO - NOTNLM OT - ellagic acid OT - gut microbiota OT - oxidative stress OT - piglets OT - tryptophan metabolism COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:34 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - antiox15101356 [pii] AID - antioxidants-15-01356 [pii] AID - 10.3390/antiox15101356 [doi] PST - epublish SO - Antioxidants (Basel). 2026 Oct 20;15(10):1356. doi: 10.3390/antiox15101356. PMID- 42939921 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3921 (Print) IS - 2076-3921 (Electronic) IS - 2076-3921 (Linking) VI - 15 IP - 10 DP - 2026 Oct 19 TI - Ultrasound-Assisted Extraction of Pleurotus ostreatus By-Products for the Development of Nutritionally Enhanced Gluten-Free Cookies: Nutritional, Sensory, and Metabolomic Insights. LID - 10.3390/antiox15101347 [doi] LID - 1347 AB - The growing demand for gluten-free (GF) products has highlighted nutritional limitations in commercial bakery products, particularly low fiber, mineral, and bioactive compound content. This study evaluated the valorization of Pleurotus ostreatus by-products as nutritionally valuable ingredients for GF cookies, focusing on the nutritional and metabolomic effects of ultrasound-assisted extraction (UAE). Untreated and UAE-treated by-products were incorporated into GF cookies at 4% and 8% (w/w). Proximate composition, mineral content, antioxidant capacity, untargeted phenolic profile, and sensory characteristics were assessed. UAE modified the nutritional and metabolomic profiles of mushroom by-products, increasing soluble dietary fiber, protein, and essential minerals such as Zn, Cu, and Mn. Untargeted metabolomic analysis revealed that UAE selectively promoted low-molecular-weight phenolic acids. Compared with a commercial GF reference, reformulated cookies exhibited higher protein and total dietary fiber contents, fulfilling the "high fiber" nutritional claim, together with a more balanced mineral profile. All reformulated cookies also showed higher iron content than the commercial GF reference, allowing the "source of iron" nutritional claim. Metabolomic profiling confirmed greater phenolic diversity and abundance in reformulated cookies, while UAE-treated by-products enhanced phenolic acids. Overall, UAE-treated mushroom by-products represent a clean-label strategy to enhance the nutritional quality and bioactive compound profile of GF bakery products. FAU - Quizhpe, Jhazmin AU - Quizhpe J AUID- ORCID: 0009-0005-0965-2835 AD - Department of Food Technology, Food Science and Nutrition, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Espinardo, 30100 Murcia, Spain. FAU - Penalver, Rocio AU - Penalver R AUID- ORCID: 0009-0006-4326-6775 AD - Department of Food Technology, Food Science and Nutrition, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Espinardo, 30100 Murcia, Spain. FAU - Ayuso, Pablo AU - Ayuso P AUID- ORCID: 0009-0008-0712-9903 AD - Department of Food Technology, Food Science and Nutrition, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Espinardo, 30100 Murcia, Spain. FAU - Zhang, Leilei AU - Zhang L AUID- ORCID: 0000-0002-9278-0459 AD - Department for Sustainable Food Process, Universita Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122 Piacenza, Italy. FAU - Garcia-Perez, Pascual AU - Garcia-Perez P AUID- ORCID: 0000-0003-1798-6235 AD - Department of Food Technology, Food Science and Nutrition, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Espinardo, 30100 Murcia, Spain. FAU - Lucini, Luigi AU - Lucini L AUID- ORCID: 0000-0002-5133-9464 AD - Department for Sustainable Food Process, Universita Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122 Piacenza, Italy. FAU - Nieto, Gema AU - Nieto G AUID- ORCID: 0000-0003-2349-2899 AD - Department of Food Technology, Food Science and Nutrition, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Espinardo, 30100 Murcia, Spain. LA - eng GR - PID2021-123628OB-C44/Government of Spain/ GR - RYC2023-044123-I/Ramon y Cajal program of the Ministry of Science, Innovation and Universities, the State Research Agency/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Antioxidants (Basel) JT - Antioxidants (Basel, Switzerland) JID - 101668981 PMC - PMC13701189 OTO - NOTNLM OT - UHPLC-HRMS OT - bioactive compounds OT - functional foods OT - metabolomics OT - mushroom by-products OT - sustainable food processing COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:34 PHST- 2026/09/20 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - antiox15101347 [pii] AID - antioxidants-15-01347 [pii] AID - 10.3390/antiox15101347 [doi] PST - epublish SO - Antioxidants (Basel). 2026 Oct 19;15(10):1347. doi: 10.3390/antiox15101347. PMID- 42939910 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3921 (Print) IS - 2076-3921 (Electronic) IS - 2076-3921 (Linking) VI - 15 IP - 10 DP - 2026 Oct 16 TI - Phenolic Profiles and Antioxidant Potential of Nymphaea and Nelumbo Species: Botanical Authentication, Redox-Modulating Mechanisms, and Translational Perspectives. LID - 10.3390/antiox15101336 [doi] LID - 1336 AB - Natural products are important sources of redox-active phytochemicals, but meaningful interpretation of their biological effects requires accurate botanical and chemical characterization. The vernacular label "lotus" is applied to botanically distinct taxa belonging to the genera Nymphaea (Nymphaeaceae) and Nelumbo (Nelumbonaceae), despite their substantial taxonomic and phytochemical divergence. This review critically evaluates authenticated evidence on botanical identity, phenolic and alkaloid composition, antioxidant activity, redox-modulating mechanisms, translational relevance, and safety across selected species of both genera. Particular emphasis is placed on organ-specific phytochemical specialization, the contrasting distribution of flavonoids and benzylisoquinoline alkaloids, and the limitations of comparing antioxidant activity across heterogeneous extraction and assay systems. Available mechanistic studies implicate interconnected antioxidant-response, inflammatory, metabolic, autophagy, and glucose-handling pathways, but the evidence remains predominantly preclinical. Nelumbo nucifera is substantially better characterized than Nymphaea species, especially with respect to alkaloid chemistry, pharmacology, and toxicology, whereas evidence for Nymphaea remains more fragmented and species dependent. Botanical authentication, chemical standardization, pharmacokinetic characterization, and preparation-specific safety assessment are therefore essential for meaningful translation. Overall, the therapeutic potential of Nymphaea and Nelumbo is scientifically plausible, but current evidence is insufficient for broad clinical generalization or interchangeable use of materials marketed as "lotus". FAU - Czernicka, Maria AU - Czernicka M AUID- ORCID: 0000-0003-2977-5355 AD - Department of Bioenergetics, Food Analysis and Microbiology, Faculty of Technology and Life Sciences, University of Rzeszow, Zelwerowicza 8B St., 35-601 Rzeszow, Poland. FAU - Such, Sebastian AU - Such S AUID- ORCID: 0009-0001-4750-6012 AD - Department of Bioenergetics, Food Analysis and Microbiology, Faculty of Technology and Life Sciences, University of Rzeszow, Zelwerowicza 8B St., 35-601 Rzeszow, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Antioxidants (Basel) JT - Antioxidants (Basel, Switzerland) JID - 101668981 PMC - PMC13702011 OTO - NOTNLM OT - Nelumbo OT - Nymphaea OT - benzylisoquinoline alkaloids OT - botanical authentication OT - phenolic compounds OT - redox signaling OT - safety assessment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:34 PHST- 2026/09/13 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - antiox15101336 [pii] AID - antioxidants-15-01336 [pii] AID - 10.3390/antiox15101336 [doi] PST - epublish SO - Antioxidants (Basel). 2026 Oct 16;15(10):1336. doi: 10.3390/antiox15101336. PMID- 42939905 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3921 (Print) IS - 2076-3921 (Electronic) IS - 2076-3921 (Linking) VI - 15 IP - 10 DP - 2026 Oct 15 TI - Sulfur-Induced Thiol Metabolism Confers Arsenic Stress Tolerance Despite Increased Arsenic Accumulation in Rice Seedlings. LID - 10.3390/antiox15101331 [doi] LID - 1331 AB - Arsenic (As) contamination poses a serious threat to rice production and food safety. Although sulfur (S) nutrition is known to alleviate As toxicity through thiol-based detoxification, the mechanisms underlying the relationship between S-induced As accumulation and tolerance remain poorly understood. We investigated the effects of contrasting S levels (0, 1, and 4 mM) on As accumulation, translocation, oxidative stress responses, thiol metabolite pools, and mineral nutrient profiles in hydroponically grown rice seedlings exposed to 15 microM As for 15 days. S supplementation alleviated As-induced growth inhibition in a dose-dependent manner despite increasing total As accumulation in both roots and shoots, demonstrating that S-mediated tolerance is independent of reduced As accumulation. Elevated S supply reduced oxidative stress markers (O(2)(*-), H(2)O(2), and MDA), while markedly enlarged the pools of gamma-glutamylcysteine (gamma-EC), glutathione, and phytochelatins (PCs), particularly in roots. S supplementation also improved the uptake of major essential nutrients, including nitrogen and potassium in shoots, while selectively modulating micronutrient distributions under As stress. Correlation analysis showed that root As content was strongly and positively correlated with gamma-EC and PC2-PC4, and principal component analysis separated the treatments along the S gradient in both roots and shoots. Collectively, these results suggest that S enhances As tolerance in rice seedlings through thiol-dependent detoxification, thereby decoupling As tolerance from As accumulation rather than by restricting As accumulation. FAU - Jung, Ha-Il AU - Jung HI AUID- ORCID: 0000-0003-2357-101X AD - Soil and Water Environment Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea. FAU - Kim, Myung-Sook AU - Kim MS AD - Soil and Water Environment Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea. FAU - Lee, Eun-Jin AU - Lee EJ AUID- ORCID: 0009-0007-4959-817X AD - Soil and Water Environment Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea. FAU - Lee, Tae-Gu AU - Lee TG AUID- ORCID: 0000-0002-3115-7516 AD - Soil and Water Environment Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea. FAU - Latt, Theint Thandar AU - Latt TT AUID- ORCID: 0000-0002-1178-2496 AD - Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea. FAU - Lee, Jinwook AU - Lee J AD - Department of Plant Science and Technology, Chung-Ang University, Anseong 17546, Republic of Korea. FAU - Lwin, Chaw Su AU - Lwin CS AD - Soil and Water Environment Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of Korea. FAU - Lee, Bok-Rye AU - Lee BR AUID- ORCID: 0000-0002-1912-0390 AD - Institute of Environmentally-Friendly Agriculture (IEFA), Chonnam National University, Gwangju 61186, Republic of Korea. LA - eng GR - PJ017632, PJ017441, PJ014360/Agriculture Science & Technology Development/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Antioxidants (Basel) JT - Antioxidants (Basel, Switzerland) JID - 101668981 PMC - PMC13701989 OTO - NOTNLM OT - arsenic OT - detoxification OT - rice OT - sulfur OT - thiol metabolism COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:34 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - antiox15101331 [pii] AID - antioxidants-15-01331 [pii] AID - 10.3390/antiox15101331 [doi] PST - epublish SO - Antioxidants (Basel). 2026 Oct 15;15(10):1331. doi: 10.3390/antiox15101331. PMID- 42939895 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3921 (Print) IS - 2076-3921 (Electronic) IS - 2076-3921 (Linking) VI - 15 IP - 10 DP - 2026 Oct 13 TI - Circular RNAs in Alzheimer's Disease: Mechanisms, Biomarkers, and EPAC Signalling in Ferroptosis. LID - 10.3390/antiox15101321 [doi] LID - 1321 AB - Alzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by cognitive decline and complex pathogenesis, involving amyloid-beta (Abeta) accumulation, tau pathology, and neuroinflammation, driven by microglial shifts toward pro-inflammatory M1-like states, causing neuronal dysfunction and death, particularly by ferroptosis. Current diagnostic and therapeutic strategies remain limited, highlighting the need for novel molecular regulators. Circular RNAs (circRNAs) are covalently closed non-coding RNAs (ncRNAs), representing promising regulatory molecules and peripheral biomarkers. Evidence suggests that circRNAs regulate AD-related pathways, through microRNA (miRNA) sponging, including modulation of NF-kappaB/BACE1-dependent (nuclear-factor-kappa-B/beta-site APP-cleaving enzyme 1) amyloidogenic APP (amyloid precursor protein) processing and through disruption of NMDA (N-methyl-D-aspartate) receptor-dependent synaptic signaling, exemplified by circRIMS2-driven UBE2K (ubiquitin-conjugating enzyme E2K)-mediated GluN2B (glutamate ionotropic receptor NMDA type subunit-2B) degradation. Additionally, circRNAs represent promising non-invasive diagnostic biomarkers. Plasma hsa_circRNA_050263 showed high diagnostic accuracy for early-stage AD. CircRNAs may offer a therapeutic strategy for modulating cell-death pathways, particularly ferroptosis. Selectively targeting EPAC2 (exchange protein directly activated by cAMP2) may preserve EPAC2-associated neuroprotection, while limiting EPAC1-associated ferroptosis, although challenges such as blood-brain barrier delivery remain to be addressed. This review summarizes circRNA biogenesis, diagnostic potential, and roles in AD pathogenesis, and limitations and AD models, besides investigating the selective circRNA-EPAC axis. FAU - Yuksel, Mert AU - Yuksel M AUID- ORCID: 0009-0000-5269-8349 AD - Department of Molecular Pharmacology, University of Groningen, 9713 AV Groningen, The Netherlands. AD - Faculty of Medicine, Ataturk University, Erzurum 25240, Turkiye. FAU - Schmidt, Martina AU - Schmidt M AUID- ORCID: 0000-0003-3075-0630 AD - Department of Molecular Pharmacology, University of Groningen, 9713 AV Groningen, The Netherlands. AD - Groningen Research Institute for Asthma and COPD (GRIAC), University Medical Center Groningen, University of Groningen, 9713 AV Groningen, The Netherlands. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Antioxidants (Basel) JT - Antioxidants (Basel, Switzerland) JID - 101668981 PMC - PMC13700829 OTO - NOTNLM OT - Alzheimer's disease OT - Amyloid-beta plaques OT - EPAC OT - circular RNA OT - ferroptosis OT - neuroinflammation OT - tau protein COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:34 PHST- 2026/09/06 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - antiox15101321 [pii] AID - antioxidants-15-01321 [pii] AID - 10.3390/antiox15101321 [doi] PST - epublish SO - Antioxidants (Basel). 2026 Oct 13;15(10):1321. doi: 10.3390/antiox15101321. PMID- 42939892 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 2076-3921 (Print) IS - 2076-3921 (Electronic) IS - 2076-3921 (Linking) VI - 15 IP - 10 DP - 2026 Oct 13 TI - IV Vitamin C with Chemotherapy for Cisplatin-Ineligible Bladder Cancer Patients: A Forgotten Group. LID - 10.3390/antiox15101318 [doi] LID - 1318 AB - Purpose: Neoadjuvant cisplatin-based chemotherapy (NAC) is considered the standard of care (SOC) for patients with muscle-invasive bladder cancer (MIBC). However, approximately 50% of patients are cisplatin-ineligible (CI) and often proceed directly to surgery. We initiated a phase Ib/IIa trial to evaluate high-dose intravenous ascorbate (IVC) with gemcitabine/carboplatin (Gem/Carbo) as NAC for patients with CI-MIBC (NCT04046094). Patients and Methods: Patients with newly diagnosed CI-MIBC received a single cycle of Gem (1000 mg/m(2) D1, 8)/Carbo (AUC 5 d 1) and IVC (25-125 g titrated to 350-400 mg/dL blood concentrations 2 x per week for 21 days) followed by definitive cystectomy. The primary outcome was pathological downstaging (/=8-point improvement in overall knee injury and osteoarthritis outcome score (KOOS). Exploratory proteomic profiling was performed in a subgroup of 33 patients (13 responders and 20 non-responders) using platelet-rich plasma-derived platelet lysate (PRP-PL) generated from the same blood-derived preparation used for treatment. Samples were analyzed by data-independent acquisition mass spectrometry, followed by a bioinformatic analysis of differential proteins and enriched pathways. To further investigate selected proteomic findings, IgA levels were quantified in PRP-PL from the entire cohort. RESULTS: Responders showed significant improvements in pain, overall KOOS, and functional outcomes. Proteomic analysis identified a non-responder signature characterized by increased immunoglobulin chains, complement-related proteins, acute-phase molecules, extracellular matrix remodeling proteins, and metabolic stress markers. Functional enrichment revealed the activation of immune, inflammatory, and tissue remodeling pathways. IgA levels in PRP-PL were significantly higher in non-responders, consistent with the immunoglobulin-related proteomic findings. CONCLUSIONS: PRGF non-response is associated with an inflammatory and immune-related phenotype. Higher IgA levels in PRP-PL were associated with reduced treatment responsiveness and may help identify a biological phenotype less likely to benefit from PRGF treatment. FAU - Colombini, Alessandra AU - Colombini A AUID- ORCID: 0000-0002-1800-3424 AD - Orthopaedic Biotechnology Lab, IRCCS Galeazzi-Sant'Ambrogio Hospital, 20157 Milan, Italy. FAU - Raffo, Vincenzo AU - Raffo V AUID- ORCID: 0009-0004-4658-722X AD - Department of Precision and Regenerative Medicine and Ionian Area (Di. Me. Pre-J), University of Bari, 70124 Bari, Italy. FAU - De Vecchi, Elena AU - De Vecchi E AUID- ORCID: 0000-0001-9781-8607 AD - Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi-Sant'Ambrogio Hospital, 20157 Milan, Italy. FAU - Pandolfi, Laura AU - Pandolfi L AD - Laboratory of Clinical Chemistry and Microbiology, IRCCS Galeazzi-Sant'Ambrogio Hospital, 20157 Milan, Italy. FAU - Marasco, Emiliano AU - Marasco E AD - Division of Rheumatology, Bambino Gesu Children's Hospital, 00165 Rome, Italy. FAU - Grieco, Giulio AU - Grieco G AD - Orthopaedic Biotechnology Lab, IRCCS Galeazzi-Sant'Ambrogio Hospital, 20157 Milan, Italy. FAU - Landoni, Simona AU - Landoni S AD - Orthopaedic Biotechnology Lab, IRCCS Galeazzi-Sant'Ambrogio Hospital, 20157 Milan, Italy. FAU - Lo Pinto, Margot AU - Lo Pinto M AUID- ORCID: 0009-0003-0394-9414 AD - Proteomics Group of Ri. MED Foundation, Research Department IRCCS ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), 90133 Palermo, Italy. FAU - Scilabra, Simone Dario AU - Scilabra SD AUID- ORCID: 0000-0001-7300-6153 AD - Proteomics Group of Ri. MED Foundation, Research Department IRCCS ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), 90133 Palermo, Italy. FAU - de Girolamo, Laura AU - de Girolamo L AUID- ORCID: 0000-0002-9979-3092 AD - Orthopaedic Biotechnology Lab, IRCCS Galeazzi-Sant'Ambrogio Hospital, 20157 Milan, Italy. LA - eng GR - Ricerca corrente/Ministry of Health/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Biomolecules JT - Biomolecules JID - 101596414 RN - 0 (Intercellular Signaling Peptides and Proteins) RN - 0 (Biomarkers) RN - 0 (Proteome) SB - IM MH - Humans MH - *Osteoarthritis, Knee/drug therapy/metabolism/immunology/therapy MH - *Proteomics/methods MH - *Platelet-Rich Plasma/metabolism MH - Female MH - Male MH - Middle Aged MH - *Intercellular Signaling Peptides and Proteins/therapeutic use/administration & dosage MH - Aged MH - Biomarkers/blood MH - Inflammation/metabolism MH - Proteome MH - *Blood Platelets/metabolism PMC - PMC13702774 OTO - NOTNLM OT - biomarkers OT - immunoglobulin A OT - inflammation OT - knee osteoarthritis OT - orthobiologics OT - plasma rich in growth factors (PRGF) OT - proteomics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/12 CRDT- 2026/10/27 10:34 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - biom16101482 [pii] AID - biomolecules-16-01482 [pii] AID - 10.3390/biom16101482 [doi] PST - epublish SO - Biomolecules. 2026 Oct 12;16(10):1482. doi: 10.3390/biom16101482. PMID- 42939734 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-273X (Electronic) IS - 2218-273X (Linking) VI - 16 IP - 10 DP - 2026 Oct 12 TI - Role of Extrachromosomal Circular DNA in Sex Reversal of Monopterus albus. LID - 10.3390/biom16101480 [doi] LID - 1480 AB - Extrachromosomal circular DNA (eccDNA) serves as a vital regulator of gene expression and phenotypic plasticity. The Monopterus albus is a protogynous hermaphroditic fish, and the functional role of eccDNA during its female-to-male sex reversal remains poorly understood. We conducted Circle-seq analysis on gonadal tissues of super-female and male individuals and integrated our findings with previously published gonadal RNA-seq data. A total of 62,574 and 34,562 eccDNAs were, on average, identified in the two groups, and 7881 down-regulated and 2738 up-regulated differentially abundant eccDNAs were screened in male gonads. Three candidate eccDNAs, eccDNA(Dmrt1a), eccDNA(Sox9a1) and eccDNA(ZNF239), were selected for subsequent analysis. Quantitative real-time PCR was applied to detect the overall transcriptional levels of their corresponding host genes, covering transcripts derived from both genomic DNA and eccDNA. Spatiotemporal expression profiling and letrozole induction experiments revealed male-biased expression of Dmrt1a and Sox9a1, while ZNF239 was predominantly expressed in female gonads. Complete sex reversal was observed in female M. albus after 90-day letrozole treatment, accompanied by elevated Dmrt1a/Sox9a1 and reduced ZNF239 transcription. Fluorescence in situ hybridization localized these transcripts in gonadal germ cells and somatic cells across developmental stages. This study verifies the production of eccDNA during gonadal development. The results indicate that Dmrt1a and Sox9a1 may facilitate sex reversal and suggest that ZNF239 is likely involved in ovarian development. This work provides novel clues for deciphering the molecular mechanism underlying sex reversal from the eccDNA perspective. FAU - Fang, Junchao AU - Fang J AD - State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. AD - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China. FAU - Li, Guanglve AU - Li G AD - State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. FAU - Luo, Wenyin AU - Luo W AD - State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. FAU - Tian, Haifeng AU - Tian H AUID- ORCID: 0000-0002-8264-5579 AD - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China. FAU - Hu, Qiaomu AU - Hu Q AUID- ORCID: 0000-0002-6497-9735 AD - State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. AD - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China. LA - eng GR - 2023BBB172/Key R&D Program of Hubei Province/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Biomolecules JT - Biomolecules JID - 101596414 RN - 0 (Extrachromosomal DNA) RN - 0 (DNA, Circular) RN - 0 (DMRT1 protein) RN - 0 (Transcription Factors) RN - 0 (SOX9 Transcription Factor) SB - IM MH - Animals MH - Male MH - Female MH - Extrachromosomal DNA/genetics MH - *DNA, Circular/genetics MH - Gonads/metabolism/growth & development MH - *Sex Determination Processes/genetics MH - *Smegmamorpha/genetics MH - Transcription Factors/genetics/metabolism MH - SOX9 Transcription Factor/genetics/metabolism PMC - PMC13702476 OTO - NOTNLM OT - Monopterus albus OT - extrachromosomal circular DNA OT - hermaphroditic fish OT - sex differentiation genes OT - sex reversal COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/12 CRDT- 2026/10/27 10:34 PHST- 2026/07/31 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - biom16101480 [pii] AID - biomolecules-16-01480 [pii] AID - 10.3390/biom16101480 [doi] PST - epublish SO - Biomolecules. 2026 Oct 12;16(10):1480. doi: 10.3390/biom16101480. PMID- 42939733 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-273X (Electronic) IS - 2218-273X (Linking) VI - 16 IP - 10 DP - 2026 Oct 11 TI - Artificial Intelligence-Driven Nanobody Engineering for Pathogen Recognition: From Functional Epitope Prioritization to Multimodal Foundation Models. LID - 10.3390/biom16101479 [doi] LID - 1479 AB - Nanobodies are compact and stable proteins derived from the variable domain of camelid heavy-chain-only antibodies (VHH). Advances in structural biology, molecular modeling, and artificial intelligence (AI) are shifting VHH discovery from empirical selection toward data-driven design. However, predicted binding alone does not ensure biological activity or successful translation, particularly for antigenically variable pathogens. Complementing recent reviews that broadly survey computational VHH modeling and optimization, this review focuses on how antigen biology and functional epitope prioritization can guide the design of pathogen-targeting VHHs. We organize the field along a structure-function-translation continuum, connecting the structural basis of VHH recognition and available data resources with conventional discovery, antigen-guided de novo design, and sequence-structure learning. We further examine how developability, recombinant production, multispecificity, humanization, structural dynamics, and antigenic variation shape the progression from predicted binders to functional VHHs. Finally, we discuss how multimodal foundation models could integrate these dimensions to support the design of functional, manufacturable, and escape-resilient VHHs. FAU - Hu, Rui-Si AU - Hu RS AUID- ORCID: 0000-0003-0587-0542 AD - Innovation Team for Information Science and Biomedicine, School of Medicine, Sichuan University of Arts and Science, Dazhou 635000, China. FAU - Ehsan, Muhammad AU - Ehsan M AUID- ORCID: 0000-0001-5314-5869 AD - Department of Parasitology, Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan. FAU - Luo, Wei AU - Luo W AD - Innovation Team for Information Science and Biomedicine, School of Medicine, Sichuan University of Arts and Science, Dazhou 635000, China. FAU - Zhou, Jian AU - Zhou J AD - Innovation Team for Information Science and Biomedicine, School of Medicine, Sichuan University of Arts and Science, Dazhou 635000, China. FAU - Xiao, Yong-Le AU - Xiao YL AD - Innovation Team for Information Science and Biomedicine, School of Medicine, Sichuan University of Arts and Science, Dazhou 635000, China. FAU - Wang, He-Xin AU - Wang HX AD - Innovation Team for Information Science and Biomedicine, School of Medicine, Sichuan University of Arts and Science, Dazhou 635000, China. FAU - Li, Cheng-Yang AU - Li CY AD - Innovation Team for Information Science and Biomedicine, School of Medicine, Sichuan University of Arts and Science, Dazhou 635000, China. FAU - Gu, Kui AU - Gu K AD - School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing 400054, China. LA - eng GR - 2026NSFSC1448/Natural Science Foundation of Sichuan Province/ PT - Journal Article PT - Review DEP - 20261011 PL - Switzerland TA - Biomolecules JT - Biomolecules JID - 101596414 RN - 0 (Single-Domain Antibodies) RN - 0 (Epitopes) SB - IM MH - *Single-Domain Antibodies/chemistry/immunology/genetics MH - *Artificial Intelligence MH - *Epitopes/immunology/chemistry MH - Humans MH - Animals MH - *Protein Engineering/methods MH - Models, Molecular PMC - PMC13702580 OTO - NOTNLM OT - artificial intelligence OT - de novo design OT - functional epitope prioritization OT - multimodal learning OT - nanobody OT - pathogen recognition COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/10/11 CRDT- 2026/10/27 10:34 PHST- 2026/08/30 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - biom16101479 [pii] AID - biomolecules-16-01479 [pii] AID - 10.3390/biom16101479 [doi] PST - epublish SO - Biomolecules. 2026 Oct 11;16(10):1479. doi: 10.3390/biom16101479. PMID- 42939730 OWN - NLM STAT- MEDLINE DCOM- 20261027 LR - 20261028 IS - 2218-273X (Electronic) IS - 2218-273X (Linking) VI - 16 IP - 10 DP - 2026 Oct 10 TI - Computationally Guided Design and Experimental Validation of ssDNA Aptamers Targeting the SARS-CoV-2 Spike Protein. LID - 10.3390/biom16101476 [doi] LID - 1476 AB - Aptamer development has traditionally relied on iterative, labor-intensive experimental approaches. Recent advances in bioinformatics and computational power now enable the rational in silico design of high-affinity nucleic acid ligands, drastically reducing time and cost. Here, we present an integrated computational pipeline for designing ssDNA aptamers targeting the receptor-binding domain (RBD) of the SARS-CoV-2 Spike (S) protein. A library of 4000 randomly generated 20-nucleotide sequences with defined base ratios (C:G:A:T = 3:3:2:2) was screened based on secondary structure and free energy. The 100 most stable sequences (DeltaG /= 0.90). This divergence was concentrated among enriching trajectories, in which dominant clonotypes reached substantially higher relative abundance in virion ssDNA than in intracellular dsDNA. Antigen-reactive clonotypes were also preferentially shifted toward the ssDNA-biased fraction, with a median log(10)(ssDNA/dsDNA) ratio of 0.44 compared with -0.17 for non-reactive clonotypes at round 3 (p = 6.3 x 10(-8)) and round 4 (0.41 vs. -0.12, p = 1.1 x 10(-6)). CONCLUSIONS: dsDNA and ssDNA tell nearly the same story early in bio-panning but can provide markedly different accounts once selection becomes concentrated. In this selection campaign, template choice materially affected interpretation of antibody phage-display sequencing, suggesting that virion ssDNA or paired-template analysis may be preferable to dsDNA alone when late-round abundance is used for candidate prioritization. Because these results come from a single campaign against one antigen, they are descriptive of this dataset and require validation across antigens, scaffolds and display systems. FAU - Yang, Hyunji AU - Yang H AUID- ORCID: 0000-0002-1385-5177 AD - Department of Biochemistry and Molecular Biology, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea. AD - Cancer Research Institute, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea. AD - Department of Biomedical Science, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea. FAU - Lee, Hwa Kyoung AU - Lee HK AUID- ORCID: 0000-0002-9237-5656 AD - Department of Biomedical Science, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea. FAU - Ryu, Taehoon AU - Ryu T AUID- ORCID: 0000-0003-4634-5189 AD - ATG Lifetech Inc., A-909, Lionsvalley, 168, Gasandigital 1-ro, Geumcheon-gu, Seoul 08507, Republic of Korea. FAU - Yoo, Duck-Kyun AU - Yoo DK AUID- ORCID: 0000-0002-3341-4897 AD - Department of Biomedical Science, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea. FAU - Chung, Junho AU - Chung J AD - Department of Biochemistry and Molecular Biology, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea. AD - Cancer Research Institute, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea. AD - Department of Biomedical Science, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea. LA - eng GR - RS-2024-00397865/Ministry of Science and ICT/ GR - RS-2025-02215541/Ministry of Science and ICT/ GR - RS-2025-25461991/Ministry of Health and Welfare/ GR - 2710086729/RS-2025-02172968/Ministry of Health and Welfare/ GR - N10260100/Ministry of Science and ICT/ PT - Journal Article DEP - 20260922 PL - Switzerland TA - Biomolecules JT - Biomolecules JID - 101596414 RN - 0 (Peptide Library) RN - 0 (DNA, Single-Stranded) RN - 0 (Single-Chain Antibodies) RN - 0 (Complementarity Determining Regions) RN - 9007-49-2 (DNA) SB - IM MH - *High-Throughput Nucleotide Sequencing/methods MH - *Peptide Library MH - DNA, Single-Stranded/genetics MH - *Single-Chain Antibodies/genetics MH - Complementarity Determining Regions/genetics MH - DNA/genetics PMC - PMC13702184 OTO - NOTNLM OT - antibody discovery OT - antibody engineering OT - bio-panning OT - phage display OT - phagemid sequencing COIS- Author Taehoon Ryu was employed by the company ATG Lifetech Inc. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 22:05 PMCR- 2026/09/22 CRDT- 2026/10/27 10:34 PHST- 2026/08/18 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/27 22:05 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - biom16101379 [pii] AID - biomolecules-16-01379 [pii] AID - 10.3390/biom16101379 [doi] PST - epublish SO - Biomolecules. 2026 Sep 22;16(10):1379. doi: 10.3390/biom16101379. PMID- 42939626 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 21 TI - Impact of Sound and Music on Cancer Cell Viability-Current Evidence and Mechanistic Insights. LID - 10.3390/cimb48101088 [doi] LID - 1088 AB - Music therapy is established in oncology as a supportive intervention to reduce anxiety, pain, distress and treatment-related symptom burden. Beyond these clinical effects, emerging experimental evidence suggests that audible sound and music may also exert direct biological effects on non-auditory cells, including cancer cells. This review summarizes current evidence on the effects of audible sound, music and acoustic stimulation on cancer-cell viability and related cellular processes. Sound represents a mechanical pressure wave that may interact with cells through mechanotransductive processes involving the plasma membrane, cytoskeleton, integrins, focal adhesions and mechanosensitive ion channels. Since cancer cells frequently display altered stiffness, cytoskeletal organization, membrane dynamics and mechanosensitivity, they may respond differently to acoustic or vibrational stimulation than non-malignant cells. Available in vitro studies report associations among audible sound, music, acoustic stimulation and cancer-cell viability, proliferation, migration, apoptosis-related signalling, gene expression and extracellular vesicle secretion. These effects have been demonstrated in breast, gastric, prostate, glioblastoma, colon, ovarian and neuroblastoma cell models, but findings appear highly cell-type-specific and dependent on exposure parameters. Proposed mechanisms include intracellular calcium signalling, cytoskeletal remodelling, mitochondrial dysfunction, reactive oxygen species generation and pathways involving PIEZO1, integrins, focal adhesion kinase and YAP/TAZ; however, causal mechanisms are not shown. Much of the mechanistic framework derives from ultrasound-based research and cannot be extrapolated to audible sound or music. Overall, the reported findings of in vitro experiments on the effect of sound on cancer cells remain preliminary, methodologically heterogeneous and do not establish reproducible, causal or cancer-selective effects. Standardized studies are required before these observations can be translated into mechanistically defined or therapeutically relevant concepts in oncology. FAU - Jungraithmayr, Wolfgang AU - Jungraithmayr W AUID- ORCID: 0000-0002-1442-4862 AD - Division of Thoracic Surgery, Rostock University Medical Center, Schillingallee 35, 18057 Rostock, Germany. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703310 OTO - NOTNLM OT - cancer cells OT - music OT - sound OT - viability COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:34 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - cimb48101088 [pii] AID - cimb-48-01088 [pii] AID - 10.3390/cimb48101088 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 21;48(10):1088. doi: 10.3390/cimb48101088. PMID- 42939624 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 20 TI - Diabetes and Cancer-Pathophysiology and Clinical Implications: A Systematic Review. LID - 10.3390/cimb48101086 [doi] LID - 1086 AB - Diabetes Mellitus and cancer are major global health problems with rising incidence worldwide. This systematic review was conducted following PRISMA 2020 guidelines by searching PubMed, Scopus, ScienceDirect, SpringerLink, and Google Scholar for studies published between 2015 and 2026. Studies investigating the association between diabetes mellitus and cancer were selected based on predefined criteria, and data were synthesized using a narrative approach. The findings indicate a strong and mechanistically complex association between diabetes mellitus and multiple cancers, particularly pancreatic, breast, colorectal, and prostate cancers. Hyperglycemia, insulin resistance, and chronic inflammation are consistently implicated in tumor initiation and progression through key signaling pathways, including Wnt/beta-catenin, PI3K/AKT/mTOR, and AMPK. A bidirectional relationship is most evident in pancreatic cancer, highlighting its unique metabolic and oncogenic interplay. Shared molecular mediators such as cytokines, microRNAs, and metabolic regulators further support a common pathogenic link. Metformin, an antidiabetic drug, demonstrated potential anticancer effects via modulation of these interconnected pathways. Overall, this review underscores a biologically intertwined relationship between diabetes mellitus and cancer, supporting the development of integrated therapeutic strategies targeting shared metabolic and signaling mechanisms. FAU - Hasan, Zahid AU - Hasan Z AUID- ORCID: 0009-0004-4236-5451 AD - Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh. FAU - Moni, Zinnat Ara AU - Moni ZA AD - Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh. FAU - Kamal, Tasnima AU - Kamal T AD - Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh. FAU - Hossain, Md Matakabbir AU - Hossain MM AD - Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh. FAU - Kabir, Yearul AU - Kabir Y AD - Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh. FAU - Islam, Farhadul AU - Islam F AUID- ORCID: 0000-0001-5262-4702 AD - Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh. AD - School of Medicine and Dentistry, Griffith University, Gold Coast Campus, Southport, QLD 4222, Australia. LA - eng PT - Journal Article PT - Review DEP - 20261020 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703320 OTO - NOTNLM OT - cancer pathogenesis OT - chronic inflammation OT - hyperglycemia OT - insulin resistance OT - metabolic disorder OT - metformin OT - unfolded protein response (UPR) COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:34 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - cimb48101086 [pii] AID - cimb-48-01086 [pii] AID - 10.3390/cimb48101086 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 20;48(10):1086. doi: 10.3390/cimb48101086. PMID- 42939623 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 20 TI - Direct Oral Anticoagulants in the Cardiorenal Patient: Pharmacology, Kidney Function Estimation and Dose Selection Across the CKD Spectrum. LID - 10.3390/cimb48101085 [doi] LID - 1085 AB - Atrial fibrillation (AF) and chronic kidney disease (CKD) are not two coincident diseases but two expressions of the cardiorenal syndrome, the disorder in which dysfunction of the heart or the kidney induces dysfunction of the other. Their coexistence spans the chronic renocardiac and cardiorenal subtypes of that syndrome, and fits neither cleanly: the relationship is genuinely bidirectional, and each organ accelerates the deterioration of the other. Anticoagulation sits precisely at the point where this bidirectional relationship becomes a therapeutic dilemma: the same patient is simultaneously hypercoagulable and haemorrhagically fragile, and the drug intended to protect the brain is cleared by an organ whose function is both impaired and unstable. Direct oral anticoagulants (DOACs) have redefined therapeutic standards in AF and venous thromboembolism (VTE), offering predictable pharmacokinetics, fewer food and drug interactions and reduced monitoring requirements compared with vitamin K antagonists (VKAs). In the cardiorenal patient, however, these advantages are conditional. Renal elimination varies more than three-fold across the class-from approximately 27% for apixaban to approximately 80% for dabigatran-so that the choice of molecule, and not merely the decision to anticoagulate, becomes a kidney-related decision. The cardiorenal argument in favour of DOACs extends beyond bleeding: VKAs promote vascular calcification through inhibition of matrix Gla protein, are associated with calciphylaxis, and can cause anticoagulant-related nephropathy, an under-recognised cause of accelerated loss of glomerular filtration rate (GFR). Randomised and observational data indicate that GFR declines faster on VKAs than on DOACs, making the kidney an outcome of anticoagulation and not only a route of elimination. A second, largely unresolved problem is metrological rather than pharmacological. Dose thresholds for DOACs were derived from creatinine clearance (CrCl) estimated with the Cockcroft-Gault (CG) equation, whereas contemporary nephrology stages kidney disease with estimated GFR (eGFR) from the CKD-EPI or EKFC equations. The two quantities are not interchangeable, and the discordance between them translates directly into discordant doses in 4-34% of patients depending on the agent, with the largest discrepancies in the oldest patients and in those with the lowest measured function. This review reframes DOAC therapy as a cardiorenal problem. We examine the shared pathophysiological substrate of AF and CKD, the pharmacology of each agent through the lens of renal handling, the evidence across CKD stages G1-G5D including dialysis, the kidney as an outcome rather than only a covariate of anticoagulation, and the clinical consequences of the equation used to estimate kidney function at the point of prescription. FAU - Sorce, Alessandra AU - Sorce A AD - Department of Health Promotion, Mother and Child Care, Internal and Specialistic Medicine, University of Palermo, 90127 Palermo, Italy. FAU - Ciuppa, Maria Elena AU - Ciuppa ME AUID- ORCID: 0009-0007-8245-9219 AD - Department of Health Promotion, Mother and Child Care, Internal and Specialistic Medicine, University of Palermo, 90127 Palermo, Italy. FAU - Germana, Roberta AU - Germana R AD - Department of Health Promotion, Mother and Child Care, Internal and Specialistic Medicine, University of Palermo, 90127 Palermo, Italy. FAU - Altieri, Giancarlo AU - Altieri G AUID- ORCID: 0009-0006-5595-6001 AD - Department of Health Promotion, Mother and Child Care, Internal and Specialistic Medicine, University of Palermo, 90127 Palermo, Italy. FAU - Zinno, Claudio AU - Zinno C AUID- ORCID: 0009-0007-2990-378X AD - Department of Health Promotion, Mother and Child Care, Internal and Specialistic Medicine, University of Palermo, 90127 Palermo, Italy. FAU - Zumbino, Carmelo AU - Zumbino C AD - Department of Emergency, "Paolo Giaccone" University Hospital, 90100 Palermo, Italy. FAU - Puccia, Giuseppe AU - Puccia G AD - Division of Cardiology, "Paolo Giaccone" University Hospital, 90100 Palermo, Italy. FAU - Evola, Salvatore AU - Evola S AD - Catheterization Laboratory, Department of Medicine and Cardiology, Azienda Ospedaliera Universitaria Policlinico "P. Giaccone", 90127 Palermo, Italy. FAU - Mule, Giuseppe AU - Mule G AUID- ORCID: 0000-0002-8500-8671 AD - Department of Health Promotion, Mother and Child Care, Internal and Specialistic Medicine, University of Palermo, 90127 Palermo, Italy. FAU - Carollo, Caterina AU - Carollo C AUID- ORCID: 0000-0002-6767-8823 AD - Department of Health Promotion, Mother and Child Care, Internal and Specialistic Medicine, University of Palermo, 90127 Palermo, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261020 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703630 OTO - NOTNLM OT - CKD-EPI OT - Cockcroft-Gault OT - anticoagulant-related nephropathy OT - atrial fibrillation OT - cardiorenal syndrome OT - chronic kidney disease OT - direct oral anticoagulants OT - glomerular filtration rate OT - renocardiac syndrome COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:34 PHST- 2026/09/18 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - cimb48101085 [pii] AID - cimb-48-01085 [pii] AID - 10.3390/cimb48101085 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 20;48(10):1085. doi: 10.3390/cimb48101085. PMID- 42939619 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 19 TI - Brain Blindness Versus Eye Blindness: Genetic and Molecular Connections Between Alzheimer's Disease and Visual Impairment. LID - 10.3390/cimb48101081 [doi] LID - 1081 AB - Visual dysfunctions were observed among Alzheimer's disease (AD) patients, including posterior cortical degeneration, retinal abnormalities, or coexisting ocular disease. Posterior cortical atrophy (PCA) is a clinical syndrome characterized by progressive higher-order visual impairment and is most frequently associated with AD pathology, although other neurodegenerative causes may be possible. Pathogenic variants in PSEN1 and PSEN2 were reported in individual cases of PCA and may support a possible monogenic contribution to selected early-onset posterior phenotypes. By contrast, variants and common AD risk genes, including GRN, MAPT, TREM2, BIN1, PICALM, ABCA7, and APOE E4 allele, represented broader genetic associations whose specific contribution to posterior cortical vulnerability remains incompletely defined. These forms of evidence should be interpreted separately. Genome-wide association studies identified overlapping loci associated with AD and glaucoma, including loci on chromosome 11 involving MTCH2, NDUFS3, and SPI1. This overlap reflected shared genetic susceptibility, pleiotropy, or putative convergent biological pathways involving mitochondrial function, immune regulation, and cellular stress. However, shared loci and pathway associations did not establish direct signaling, anatomical communication, or a causal relationship between brain and ocular tissues. The opposing tissue-specific effects reported for APOE further indicated that data in the brain may not be extrapolated to the retina. Future studies should clarify the cell-specific effects, causal direction, and clinical significance of shared genetic factors using well-characterized cohorts and tissue-specific experimental models. Overall, current evidence supported the partially overlapping genetic and cellular mechanisms in AD and selected ocular disorders, while further studies should be needed on the biological relationship between the brain and eye. FAU - Bagyinszky, Eva AU - Bagyinszky E AUID- ORCID: 0000-0002-0487-8668 AD - College of BioNano Technology, Gachon University, Seongnam 13120, Republic of Korea. FAU - An, Seong Soo A AU - An SSA AUID- ORCID: 0000-0002-8123-6183 AD - Department of Bionano Technology, Gachon Medical Research Institute, Gachon University, Seongnam 13120, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703017 OTO - NOTNLM OT - Alzheimer's disease OT - age-related macular degeneration OT - genetics OT - glaucoma OT - mutations OT - posterior cortical atrophy (PCA) OT - visual impairment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:34 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - cimb48101081 [pii] AID - cimb-48-01081 [pii] AID - 10.3390/cimb48101081 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 19;48(10):1081. doi: 10.3390/cimb48101081. PMID- 42939617 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 19 TI - Molecular Mechanisms Linking Obesity and Type 2 Diabetes: Pathogenesis, Biomarkers, and Therapeutic Potential. LID - 10.3390/cimb48101079 [doi] LID - 1079 AB - Obesity and diabetes are major global health burdens, with their prevalence projected to continue rising. The comorbidity of obesity and diabetes increases the risk of serious complications and mortality, highlighting the need for early prevention and intervention. However, the precise mechanisms underlying their relationship remain unclear. A review of ten articles selected from PubMed and published from 2016 to 2026 was conducted to study the pathogenesis of type 2 diabetes (T2D) in the context of obesity and to identify biomarkers with potential for novel treatment strategies. Circulating myeloperoxidase (MPO), piezo-type mechanosensitive ion channel component 1 (PIEZO1), branched-chain amino acids, particularly BCKDH phosphatase, and lectins, such as Gal-4, may serve as inflammatory markers in comorbid obesity and T2D. alpha-2-Macroglobulin (alpha2-MG) may be a possible predictive marker in the comorbidity. Angiopoietin-like protein 6 (ANGPTL6) levels may be involved in the T2D pathway. Macrophage and THP-1 exosomes have been shown to be involved in the regulation of inflammation and reduce the expression of proinflammatory genes in mouse models. A thorough understanding of the pathways linking diabetes and obesity is essential for effective prevention, diagnosis, and treatment in T2D patients with obesity. Further research into these biomarkers may contribute to improved patient outcomes and more effective therapeutics. FAU - Han, Jiwon AU - Han J AUID- ORCID: 0000-0002-7857-8000 AD - College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA 91766, USA. FAU - Kamat, Apeksha AU - Kamat A AD - College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA 91766, USA. FAU - Nguyen, Jasmin AU - Nguyen J AD - College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA 91766, USA. FAU - Nguyen, Phat AU - Nguyen P AD - College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA 91766, USA. FAU - Vu, Katelyn AU - Vu K AUID- ORCID: 0009-0004-1439-0350 AD - College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA 91766, USA. FAU - Zhao, Melissa AU - Zhao M AD - College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA 91766, USA. FAU - Venketaraman, Vishwanath AU - Venketaraman V AUID- ORCID: 0000-0002-2586-1160 AD - College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA 91766, USA. LA - eng PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703753 OTO - NOTNLM OT - biomarkers OT - diabetes OT - inflammation OT - obesity COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:34 PHST- 2026/09/12 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - cimb48101079 [pii] AID - cimb-48-01079 [pii] AID - 10.3390/cimb48101079 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 19;48(10):1079. doi: 10.3390/cimb48101079. PMID- 42939615 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 19 TI - Identification of BRD4 as a Shared Diagnostic Biomarker Between Bipolar Disorder and Multiple Sclerosis Using Machine Learning. LID - 10.3390/cimb48101077 [doi] LID - 1077 AB - Bipolar disorder (BD) is closely associated with multiple sclerosis (MS), but their shared molecular mechanisms and specific diagnostic biomarkers remain unclear. This study integrated microarray expression profiling and machine learning to identify shared core genes and underlying mechanisms between BD and MS. Gene expression datasets and peripheral-blood transcriptome datasets were obtained from the GEO database. After per-cohort quantile normalization, shared genes were identified by differential expression analysis and weighted gene co-expression network analysis. A protein-protein interaction network was constructed, and key genes were screened using the MCODE plugin. Three machine learning algorithms were combined to identify the final core gene. A total of 229 shared genes were initially obtained and further filtered to 55 key genes. BRD4 was identified as the final shared core gene. Discriminative performance (AUC, 95% CI) was: BD discovery GSE23848 (20 cases/15 controls) 0.713 (0.54-0.89); MS discovery GSE21942 (12/15) 0.998 (0.99-1.00); BD external GSE39653 (8/24) 0.775 (0.59-0.96); MS external GSE203241 (38/21) 0.828 (0.71-0.93). BRD4 shows moderate-to-good discriminative performance as a non-invasive peripheral biomarker and may aid further investigation of shared BD-MS mechanisms. FAU - Zhou, Xiaoyuan AU - Zhou X AD - College of Clinical Medicine, Capital Medical University Yanjing Medical College, Yanqin Building Room 207, Da dong Road 4, Shunyi District, Beijing 101300, China. FAU - Zhang, Yixuan AU - Zhang Y AD - College of Clinical Medicine, Capital Medical University Yanjing Medical College, Yanqin Building Room 207, Da dong Road 4, Shunyi District, Beijing 101300, China. FAU - Xu, Youzhi AU - Xu Y AD - College of Clinical Medicine, Capital Medical University Yanjing Medical College, Yanqin Building Room 207, Da dong Road 4, Shunyi District, Beijing 101300, China. FAU - Huo, Chunyue AU - Huo C AD - College of Clinical Medicine, Capital Medical University Yanjing Medical College, Yanqin Building Room 207, Da dong Road 4, Shunyi District, Beijing 101300, China. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13702881 OTO - NOTNLM OT - BRD4 OT - biomarker OT - bipolar disorder OT - machine learning OT - multiple sclerosis OT - neuroinflammation COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:34 PHST- 2026/09/04 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - cimb48101077 [pii] AID - cimb-48-01077 [pii] AID - 10.3390/cimb48101077 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 19;48(10):1077. doi: 10.3390/cimb48101077. PMID- 42939608 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 16 TI - The Clinical and Phenotypic Significance of Sub-Threshold JAK2 V617F Signals in Patients Classified as JAK2 V617F-Negative. LID - 10.3390/cimb48101070 [doi] LID - 1070 AB - BACKGROUND: This study investigates the relationship between clinical and hematological parameters and sub-threshold positive findings in cases with suspected hematological malignancies subjected to Janus Kinase 2 (JAK2) V617F qPCR testing. METHODS: Adult patients referred to the Medical Genetics Clinic from the Erciyes University Faculty of Medicine Hematology Department between 2022 and 2024 for JAK2 V617F analysis due to suspected hematological malignancy were evaluated in this retrospective, cross-sectional study. Registered data from 2394 patients were analyzed, and 173 cases with a JAK2 V617F allele burden below 0.1% but above the negative control value were included in the study. SPSS version 25.0 software was used for statistical analysis. RESULTS: More than half of the patients (56.6%) had no definitive hematological diagnosis at initial testing, while 28.9% developed a hematological disorder during follow-up, including overt MPN in 13.3%. Significant differences across VAF groups were observed for leukocytosis (p = 0.004), neutrophilia (p = 0.030), and monocytosis (p = 0.024), with the highest frequencies in Group 3 (0.041-0.099%). ROC analyses identified closely clustered optimal cut-offs around 0.027-0.029%, whereas no independent predictor of higher sub-threshold allele burden emerged in multivariate analysis (p > 0.05). CONCLUSIONS: Even sub-threshold levels of the JAK2 V617F mutation may exhibit specific clinical manifestations and may be associated with constitutional symptoms and leukocytosis in particular. The study findings also showed a lower awareness of the JAK2 test in clinical practice, and that it is significantly employed in off-label cases. In order to accurately interpret low-allele-burden results, molecular findings must be evaluated in conjunction with hematological parameters and appropriate clinical indications. The findings underscore the importance of applying the JAK2 V617F PCR test selectively and based on clinical suspicion. FAU - Unsal, Serife Emre AU - Unsal SE AUID- ORCID: 0000-0003-4581-8722 AD - Department of Hematology, Erciyes University, Kayseri 38280, Turkey. FAU - Akalin, Hilal AU - Akalin H AUID- ORCID: 0000-0002-2580-836X AD - Department of Medical Genetics, Erciyes University, Kayseri 38280, Turkey. FAU - Tan, Busra AU - Tan B AUID- ORCID: 0000-0001-6072-5570 AD - Department of Medical Genetics, Erciyes University, Kayseri 38280, Turkey. FAU - Polat Akyol, Gulsah AU - Polat Akyol G AD - Department of Hematology, Erciyes University, Kayseri 38280, Turkey. FAU - Kiraz, Aslihan AU - Kiraz A AUID- ORCID: 0000-0001-7317-2717 AD - Department of Medical Genetics, Erciyes University, Kayseri 38280, Turkey. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703430 OTO - NOTNLM OT - JAK2 V617F mutation OT - allele burden OT - hematological neoplasms COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:34 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/11 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - cimb48101070 [pii] AID - cimb-48-01070 [pii] AID - 10.3390/cimb48101070 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 16;48(10):1070. doi: 10.3390/cimb48101070. PMID- 42939607 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 16 TI - beta-Boswellic Acid Suppresses Migration and Invasion and Attenuates TGF-beta1-Induced EMT-Related Changes in PC-3 Prostate Cancer Cells. LID - 10.3390/cimb48101069 [doi] LID - 1069 AB - beta-Boswellic acid (BA) has potential antitumor activity, but its effects on migration, invasion, and epithelial-mesenchymal transition (EMT)-related phenotypes in prostate cancer cells remain unclear. In this study, we integrated public transcriptomic data, candidate molecule prediction, machine learning, functional association analysis, and molecular simulations with MTT, wound-healing, Transwell, qPCR, Western blotting, transcriptomic analysis, and data-independent acquisition proteomics to evaluate BA effects on PC-3 cells and explore its potential molecular basis. We identified 22 candidate BA-related molecules and prioritised DPP4, MME, and ABL1 for further analysis. Higher BA concentrations reduced PC-3 cell viability. At the same time, BA suppressed wound closure, migration, and invasion and attenuated TGF-beta1-induced EMT-related changes, accompanied by increased E-cadherin and decreased N-cadherin, Snail, and ZEB1 levels. DPP4, MME, and ABL1 also exhibited BA-associated expression changes. Integrated transcriptomic and proteomic analyses suggested that BA-associated molecular alterations involved the cell cycle, DNA replication, cell adhesion, cytoskeletal remodelling, and TGF-beta-related processes. In conclusion, BA suppresses migration and invasion in PC-3 cells and attenuates TGF-beta1-induced EMT-related changes. Candidate molecules including DPP4, MME, and ABL1, together with the multi-omics findings, provide exploratory clues for further investigation of the potential molecular basis of BA activity. FAU - Song, Kaixin AU - Song K AUID- ORCID: 0009-0009-2195-2127 AD - School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Zang, Kunpeng AU - Zang K AD - School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Yang, Zihan AU - Yang Z AD - School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Tan, Zhirong AU - Tan Z AD - College of Integrated Traditional Chinese and Western Medicine, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Geng, Xiaohui AU - Geng X AD - School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Wang, Jiale AU - Wang J AD - College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Zhu, Lixi AU - Zhu L AD - College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - He, Sijie AU - He S AD - College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China. FAU - Pan, Sian AU - Pan S AD - College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha 410208, China. LA - eng GR - 2024M760897/China Postdoctoral Science Foundation (76th General Program)/ GR - 24B0378/Hunan Provincial Department of Education Excellent Youth Project/ GR - 2025ZYYKF12/Open Fund of Hunan International Joint Laboratory of Traditional Chinese Medicine and Ethnomedicine/ GR - 2024BKS158/Undergraduate Research and Innovation Fund of Hunan University of Chinese Medicine/ GR - Z2023XJYB10/General Project of Hunan University of Chinese Medicine/ GR - S202410541027/Hunan Provincial Department of Education Innovation Training Program for College Students/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703607 OTO - NOTNLM OT - PC-3 cells OT - epithelial-mesenchymal transition OT - invasion OT - machine learning OT - migration OT - multi-omics OT - prostate cancer OT - beta-boswellic acid COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analysis, or interpretation of the data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:34 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - cimb48101069 [pii] AID - cimb-48-01069 [pii] AID - 10.3390/cimb48101069 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 16;48(10):1069. doi: 10.3390/cimb48101069. PMID- 42939606 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 15 TI - Baicalein Synergizes with Docetaxel in TNBC and the Combination Suppresses AKT/GSK3beta/beta-Catenin Signaling. LID - 10.3390/cimb48101066 [doi] LID - 1066 AB - Limited docetaxel efficacy in triple-negative breast cancer (TNBC) necessitates chemosensitizers. Baicalein is a potential chemosensitizer, but its synergy with docetaxel in TNBC is unclear. Here, we investigated the combined effects and underlying mechanisms. The concentrations of baicalein (80 muM) and docetaxel (20 nM) were tested on TNBC cells; synergy was assessed by CCK-8 and Chou-Talalay analysis. Proliferation, migration, invasion, and apoptosis were assessed by colony formation, wound healing, Transwell, Matrigel, and flow cytometry assays. Protein levels of p-AKT, p-GSK3beta and beta-catenin were determined by western blot, and HSP90AA1 mRNA by qPCR. Baicalein-HSP90AA1 binding was analyzed by molecular docking. The prognostic value of HSP90AA1 was analyzed by Kaplan-Meier Plotter. The combination exhibited synergistic anti-tumor activity (CI < 0.9), significantly suppressed proliferation, migration and invasion, and enhanced apoptosis. Mechanistically, the combination reduced p-AKT, p-GSK3beta, and beta-catenin protein levels without affecting HSP90AA1 mRNA. Molecular docking indicated a potential interaction between baicalein and HSP90AA1. High HSP90AA1 expression correlated with shorter distant metastasis-free survival (HR = 1.71, p = 0.0046) and worse overall survival (HR = 2.15, p = 0.021). Thus, baicalein synergizes with docetaxel to suppress TNBC in vitro, accompanied by suppression of AKT/GSK3beta/beta-catenin signaling, potentially through interaction with HSP90AA1, a proposed upstream target. FAU - Yang, Taomei AU - Yang T AD - School of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China. FAU - Chen, Xiangyun AU - Chen X AD - School of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China. FAU - Chu, Chunwei AU - Chu C AD - School of Basic Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China. FAU - Wan, Yonghu AU - Wan Y AD - Guizhou Center for Disease Control and Prevention, Guiyang 550004, China. LA - eng GR - QianKeHe Basic-ZK [2021] General 401/Guizhou Provincial Basic Research Program (Natural Science)/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703623 OTO - NOTNLM OT - AKT/GSK3beta/beta-catenin OT - HSP90AA1 OT - baicalein OT - docetaxel OT - drug synergy OT - triple-negative breast cancer COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:34 PHST- 2026/07/28 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - cimb48101066 [pii] AID - cimb-48-01066 [pii] AID - 10.3390/cimb48101066 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 15;48(10):1066. doi: 10.3390/cimb48101066. PMID- 42939605 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 15 TI - Biomarker-Enriched Host-Directed Trials in Sepsis: Integrating Immune, Endothelial, Coagulation, Redox, and Metabolic Domains. LID - 10.3390/cimb48101068 [doi] LID - 1068 AB - Sepsis is a life-threatening, biologically heterogeneous syndrome in which immune dysregulation, endothelial injury, coagulation activation, redox imbalance, and metabolic reprogramming contribute to microvascular and organ dysfunction. This structured narrative review integrates these five domains to inform biomarker-enriched trials of host-directed interventions, emphasizing the endothelium as an interface between inflammation, immunothrombosis, and impaired cellular bioenergetics. Candidate biomarkers of immune competence, endothelial injury, coagulation, and metabolism are examined alongside investigational immunomodulatory and restorative strategies. The proposed framework distinguishes three functional tiers: screening and biological enrichment; pharmacodynamic target engagement and safety assessment; and patient-centered outcomes. Candidate host-response profiles are hypothesis-generating, may overlap, and require prospective validation, with explicit provisions for mixed and indeterminate classifications. A complementary implementation model separates routine assessments from trial-specific assays and advanced mechanistic substudies, addressing sampling, reporting, missing measurements, and assay harmonization. Evidence remains heterogeneous and largely preclinical, observational, or early-phase; predictive thresholds, therapeutic windows, and patient-centered benefits remain unestablished for most candidate strategies. This framework supports prospective trial design rather than routine bedside treatment selection. Biomarker improvement must be evaluated alongside pathogen control, organ recovery, adverse events, and longer-term outcomes and should not be assumed to indicate clinical benefit. FAU - Izquierdo-Condoy, Juan S AU - Izquierdo-Condoy JS AUID- ORCID: 0000-0002-1178-0546 AD - One Health Research Group, Universidad de Las Americas, Quito 170124, Ecuador. FAU - Saavedra-Torres, Jhan S AU - Saavedra-Torres JS AUID- ORCID: 0009-0002-3643-1737 AD - Facultad de Ciencias de la Salud, Fundacion Universitaria Vision de las Americas, Pereira 660003, Colombia. FAU - Coral Gaon, Betty Liliana AU - Coral Gaon BL AD - Facultad de Ciencias de la Salud, Universidad Tecnologica de Pereira, Pereira 660003, Colombia. FAU - Gaibor-Pazmino, Alice AU - Gaibor-Pazmino A AUID- ORCID: 0000-0002-2760-8791 AD - One Health Research Group, Universidad de Las Americas, Quito 170124, Ecuador. FAU - Gil, Janeth C AU - Gil JC AD - Facultad de Salud, Universidad Santiago de Cali, Cali 760035, Colombia. FAU - Tenorio-Castro, Daniel Armando AU - Tenorio-Castro DA AUID- ORCID: 0009-0000-9047-3036 AD - Facultad de Ciencias de la Salud, Universidad del Quindio, Armenia 630004, Colombia. FAU - Grey Sayas, Angelica AU - Grey Sayas A AD - Facultad de Ciencias de la Salud, Corporacion Universitaria Empresarial Alexander Von Humboldt, Armenia 630003, Colombia. FAU - Perez Giraldo, Andrea AU - Perez Giraldo A AUID- ORCID: 0000-0002-5150-5792 AD - Facultad de Ciencias de la Salud, Corporacion Universitaria Empresarial Alexander Von Humboldt, Armenia 630003, Colombia. FAU - Garcia-Canarte, Susana AU - Garcia-Canarte S AD - Clinica Medica, Hospital Fundacion Sanatorio Guemes, Buenos Aires C1188, Argentina. FAU - Rojas-Cadena, Marlon AU - Rojas-Cadena M AUID- ORCID: 0000-0002-8445-7256 AD - One Health Research Group, Universidad de Las Americas, Quito 170124, Ecuador. FAU - Galecio Tito, Valeria Estefania AU - Galecio Tito VE AD - Departamento de Medicina Interna, Hospital de Especialdiades Eugenio Espejo, Quito 170403, Ecuador. LA - eng PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703029 OTO - NOTNLM OT - biomarkers OT - endothelial dysfunction OT - immunometabolism OT - immunothrombosis OT - mitochondrial dysfunction OT - precision medicine OT - sepsis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:34 PHST- 2026/08/25 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - cimb48101068 [pii] AID - cimb-48-01068 [pii] AID - 10.3390/cimb48101068 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 15;48(10):1068. doi: 10.3390/cimb48101068. PMID- 42939594 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 13 TI - A Programmed Cell Death Protein 1 Pathway-Derived Prognostic Transcriptomic Signature in Bladder Cancer: Development in TCGA-BLCA and Evaluation in Independent Cohorts. LID - 10.3390/cimb48101056 [doi] LID - 1056 AB - Immune checkpoint inhibitors (ICIs) targeting programmed cell death protein 1 (PD-1) and programmed death-ligand 1 (PD-L1) have changed the treatment of advanced bladder cancer, but only a minority of patients benefit. We asked whether expression of PD-1 pathway genes carries prognostic information in bladder cancer. Sixty-eight genes annotated to the PD-1 signaling, CD28 co-stimulation, and cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) inhibitory signaling pathways in Reactome were examined in The Cancer Genome Atlas bladder urothelial carcinoma cohort (TCGA-BLCA). Genes differentially expressed between tumor and normal tissue were entered into a least absolute shrinkage and selection operator (LASSO) Cox model, yielding a seven-gene risk score (FYN, GRAP2, TRIB3, MAP3K8, AKT3, CD274, and CD80). In TCGA-BLCA (n = 404), the risk score was associated with overall survival independently of age, sex, and stage (hazard ratio [HR] per standard deviation 1.24, 95% confidence interval [CI] 1.11-1.39) and modestly improved discrimination over clinical variables (concordance index [C-index] 0.630 to 0.664). With coefficients and standardization fixed in advance, the score was not associated with cancer-specific survival in GSE13507 (n = 165; HR per standard deviation 1.21, 95% CI 0.83-1.77) or with outcome in four further independent cohorts, including an atezolizumab-treated cohort; in a second, smaller ICI-treated cohort it was associated with shorter survival. Gene selection was unstable under bootstrap resampling, and the signature's associations with transcriptome-inferred immune indices were limited. This PD-1 pathway-derived signature should therefore be regarded as exploratory; its prognostic value outside TCGA-BLCA is not supported by the available evidence. FAU - Chang, Hao-Han AU - Chang HH AUID- ORCID: 0000-0002-6541-7566 AD - Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. AD - Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung 80756, Taiwan. FAU - Teng, Pai-Chi AU - Teng PC AUID- ORCID: 0000-0002-1872-466X AD - Division of Urology, Department of Surgery, Cardinal Tien Hospital, New Taipei City 23148, Taiwan. AD - Department of Urology, National Taiwan University Hospital, Taipei 10002, Taiwan. FAU - Liu, Chia-Hsin AU - Liu CH AD - Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. AD - Research Center for Cancer Biology, China Medical University, Taichung 40403, Taiwan. FAU - Lin, Yung-Lun AU - Lin YL AD - Research Center for Cancer Biology, China Medical University, Taichung 40403, Taiwan. FAU - Wang, Shu-Chi AU - Wang SC AUID- ORCID: 0000-0001-9691-7507 AD - Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. FAU - Bao, Bo-Ying AU - Bao BY AUID- ORCID: 0000-0001-5510-6513 AD - Department of Pharmacy, China Medical University, Taichung 40403, Taiwan. AD - Department of Nursing, Asia University, Taichung 41354, Taiwan. FAU - Yeh, Hsin-Chih AU - Yeh HC AUID- ORCID: 0000-0003-4113-629X AD - Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. AD - Department of Urology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. FAU - Chuu, Chih-Pin AU - Chuu CP AUID- ORCID: 0000-0001-7206-7923 AD - Institute of Cellular and System Medicine, National Health Research Institutes, Miaoli 350401, Taiwan. FAU - Geng, Jiun-Hung AU - Geng JH AUID- ORCID: 0000-0003-0610-1278 AD - Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. AD - Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung 80756, Taiwan. AD - Research Center for Environmental Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. AD - Department of Urology, Kaohsiung Municipal Siaogang Hospital, Kaohsiung 81267, Taiwan. FAU - Chang, Li-Hsin AU - Chang LH AUID- ORCID: 0000-0002-1126-7697 AD - Graduate Institute of Animal Vaccine Technology, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan. FAU - Tang, Tsz-Yi AU - Tang TY AUID- ORCID: 0000-0002-2978-6970 AD - Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. AD - Department of Urology, Kaohsiung Municipal Siaogang Hospital, Kaohsiung 81267, Taiwan. FAU - Ou, Hsien-Che AU - Ou HC AUID- ORCID: 0009-0007-6745-1147 AD - Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. AD - Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung 80756, Taiwan. FAU - Huang, Shu-Pin AU - Huang SP AUID- ORCID: 0000-0003-2968-6125 AD - Graduate Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. AD - Department of Urology, Kaohsiung Medical University Hospital, Kaohsiung 80756, Taiwan. AD - Department of Urology, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. AD - Institute of Medical Science and Technology, College of Medicine, National Sun Yat-Sen University, Kaohsiung 804201, Taiwan. FAU - Cheng, Wei-Chung AU - Cheng WC AD - Research Center for Cancer Biology, China Medical University, Taichung 40403, Taiwan. AD - Ph.D. Program for Cancer Biology and Drug Discovery, China Medical University and Academia Sinica, Taichung 40403, Taiwan. FAU - Li, Chia-Yang AU - Li CY AUID- ORCID: 0000-0001-5689-9850 AD - Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. AD - Center of Disease Multi-Omics Research, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. AD - Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 80756, Taiwan. LA - eng GR - NSTC 113-2926-I-037-501-G/National Science and Technology Council/ GR - NSTC 114-2314-B-037-006; NSTC 114-2314-B-037-026/National Science and Technology Council/ GR - KMU-DK(B)-114004-1; KT114P007/Kaohsiung Medical University/ GR - KMUH111-1M45/Kaohsiung Medical University Hospital/ GR - CMU111-IP-04; CMU113-MF-05/China Medical University/ GR - DMR-112-237/China Medical University Hospital/ GR - NSTC 115-2314-B-037-034-MY3; NSTC 115-2321-B-037-008/National Science and Technology Council/ GR - NSTC 113-2311-B-039-001-MY3 and NSTC 115-2311-B-039-002-MY3/Technology Council, Taiwan/ GR - CMU112-MF-25 and CMU114-MF-07/China Medical University/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703632 OTO - NOTNLM OT - bladder cancer OT - external evaluation OT - immune checkpoint inhibitors OT - prognostic signature OT - programmed cell death protein 1 OT - tumor microenvironment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:34 PHST- 2026/09/19 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - cimb48101056 [pii] AID - cimb-48-01056 [pii] AID - 10.3390/cimb48101056 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 13;48(10):1056. doi: 10.3390/cimb48101056. PMID- 42939593 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 13 TI - In Silico Discovery of a Potential PI3Kalpha Inhibitor Incorporating an Imidazole Moiety for Breast Cancer Treatment. LID - 10.3390/cimb48101055 [doi] LID - 1055 AB - Excessive activation of PI3Kalpha is prevalent in breast cancer, aberrantly activating the PI3K/Akt/mTOR pathway to drive tumor progression, thus rendering PI3Kalpha a vital therapeutic target. Two PI3Kalpha inhibitors (alpelisib and inavolisib) have been approved for clinical application. Inavolisib exerts a mutation-selective degradation mechanism and functions by directly eliminating aberrant proteins. To develop novel PI3Kalpha inhibitors, we employed multiple computational strategies including scaffold hopping based on the structure of inavolisib, -CDOCKER docking, Lipinski's rule of five, ADMET prediction, molecular dynamics (MD) simulation and GLORYx metabolic prediction. These tools were used to assess candidate compounds for their predicted binding modes and binding affinity with PI3Kalpha, physicochemical profiles, pharmacokinetic properties, metabolic pathways and generated metabolites. Ultimately, M-288, a computationally prioritized candidate compound and imidazole derivative with favorable predicted properties, was identified. Its alanine moiety formed a H-bond with the key residue Gln859 in the binding pocket, and the oxygen atom on its carboxyl group established a salt bridge with non-conserved Arg770 to enhance binding affinity. M-288 also formed H-bonds with Asp933, Tyr836 and Val851, facilitating its stable binding to PI3Kalpha. This compound was predicted to occupy the ATP-binding pocket. Compared with other screened candidates, it exhibited low predicted toxicity and no inhibitory activity against major hepatic CYP450 isoforms, while satisfying drug-likeness criteria and possessing favorable hepatic metabolic clearance. Molecular dynamics simulations verified its stable binding profile. This study indicates that M-288 serves as a potential PI3Kalpha inhibitor and provides valuable references for subsequent discovery of PI3Kalpha inhibitors. FAU - Jia, Wenqing AU - Jia W AD - School of Chemistry and Chemical Engineering, Qilu Normal University, Jinan 250200, China. FAU - Ma, Yukui AU - Ma Y AD - School of Chemistry and Chemical Engineering, Qilu Normal University, Jinan 250200, China. FAU - Huang, Rong AU - Huang R AD - School of Pharmacy, Binzhou Medical University, Yantai 264003, China. FAU - He, Xi AU - He X AD - School of Chemistry and Chemical Engineering, Qilu Normal University, Jinan 250200, China. FAU - Cheng, Xianchao AU - Cheng X AD - Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703664 OTO - NOTNLM OT - PI3Kalpha OT - PI3Kalpha inhibitor OT - breast cancer OT - scaffold hopping COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:34 PHST- 2026/08/21 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - cimb48101055 [pii] AID - cimb-48-01055 [pii] AID - 10.3390/cimb48101055 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 13;48(10):1055. doi: 10.3390/cimb48101055. PMID- 42939585 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 10 TI - Modeling Parkinson's Disease Through Combined DJ-1 Deficiency and Noradrenergic Degeneration. LID - 10.3390/cimb48101047 [doi] LID - 1047 AB - Parkinson's disease (PD) is characterized by progressive degeneration of the nigrostriatal dopaminergic system while increasing evidence indicates that early pathology also involves the locus coeruleus (LC) and the noradrenergic system. However, whether LC degeneration increases dopaminergic vulnerability in genetically susceptible background remains poorly understood. Here, we investigated the impact of selective noradrenergic depletion on behavioral and neuropathological phenotypes in DJ-1 knockout (KO) mice, a model of familial PD. DJ-1 KO and wild-type (WT) mice received the selective LC neurotoxin DSP-4 (50 mg/kg) to induce noradrenergic degeneration, followed by a battery of motor and non-motor behavioral assessments and histological analysis of the LC and nigrostriatal system. DSP-4 induced approximately 80-85% LC neuronal loss, confirming effective noradrenergic lesioning. While DSP-4-treated WT mice displayed relatively mild behavioral alterations, its combination with DJ-1 deficiency produced marked impairments, including increased anxious- and depressive-like behaviors, enhanced contextual fear responses, and deficits in motor coordination and balance. At the nigrostriatal level, DJ-1 KO mice already exhibited reduced TH-positive neurons in the substantia nigra pars compacta (SNc), whereas combined DJ-1 deficiency and DSP-4 treatment produced a further significant reduction in striatal TH density, without significant additional loss of SNc TH-positive neurons. These findings indicate that LC noradrenergic degeneration selectively exacerbates dopaminergic terminal pathology in the DJ-1-deficient background, suggesting increased vulnerability of the nigrostriatal system to noradrenergic loss. Overall, our data identify LC degeneration as a modifier of both motor and non-motor phenotypes associated with DJ-1 deficiency and provide experimental support for a functional interaction between the noradrenergic and nigrostriatal systems in PD. FAU - Ferreira-Antunes, Filipa AU - Ferreira-Antunes F AUID- ORCID: 0009-0000-5216-9172 AD - Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal. AD - Life and Health Sciences Research Institute (ICVS)/Biomaterials, Biodegradables and Biomimetics Research Group (3B's)-PT Government Associate Laboratory, 4710-057/4805-017 Braga/Guimaraes, Portugal. FAU - Marote, Ana AU - Marote A AUID- ORCID: 0000-0003-0090-9196 AD - Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal. AD - Life and Health Sciences Research Institute (ICVS)/Biomaterials, Biodegradables and Biomimetics Research Group (3B's)-PT Government Associate Laboratory, 4710-057/4805-017 Braga/Guimaraes, Portugal. FAU - Campos, Jonas AU - Campos J AD - Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal. AD - Life and Health Sciences Research Institute (ICVS)/Biomaterials, Biodegradables and Biomimetics Research Group (3B's)-PT Government Associate Laboratory, 4710-057/4805-017 Braga/Guimaraes, Portugal. FAU - Borges, Ines Feio AU - Borges IF AD - Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal. AD - Life and Health Sciences Research Institute (ICVS)/Biomaterials, Biodegradables and Biomimetics Research Group (3B's)-PT Government Associate Laboratory, 4710-057/4805-017 Braga/Guimaraes, Portugal. FAU - Monteiro-Fernandes, Daniela AU - Monteiro-Fernandes D AD - Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal. AD - Life and Health Sciences Research Institute (ICVS)/Biomaterials, Biodegradables and Biomimetics Research Group (3B's)-PT Government Associate Laboratory, 4710-057/4805-017 Braga/Guimaraes, Portugal. FAU - Barata-Antunes, Sandra AU - Barata-Antunes S AD - Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal. AD - Life and Health Sciences Research Institute (ICVS)/Biomaterials, Biodegradables and Biomimetics Research Group (3B's)-PT Government Associate Laboratory, 4710-057/4805-017 Braga/Guimaraes, Portugal. FAU - Mendes-Pinheiro, Barbara AU - Mendes-Pinheiro B AD - Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal. AD - Life and Health Sciences Research Institute (ICVS)/Biomaterials, Biodegradables and Biomimetics Research Group (3B's)-PT Government Associate Laboratory, 4710-057/4805-017 Braga/Guimaraes, Portugal. FAU - Salgado, Antonio J AU - Salgado AJ AUID- ORCID: 0000-0003-3806-9823 AD - Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal. AD - Life and Health Sciences Research Institute (ICVS)/Biomaterials, Biodegradables and Biomimetics Research Group (3B's)-PT Government Associate Laboratory, 4710-057/4805-017 Braga/Guimaraes, Portugal. FAU - Sampaio-Marques, Belem AU - Sampaio-Marques B AUID- ORCID: 0000-0001-6580-0971 AD - Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, 4710-057 Braga, Portugal. AD - Life and Health Sciences Research Institute (ICVS)/Biomaterials, Biodegradables and Biomimetics Research Group (3B's)-PT Government Associate Laboratory, 4710-057/4805-017 Braga/Guimaraes, Portugal. LA - eng GR - LCF/PR/HP20/52300001/Fundacion La Caixa/ GR - UID/06304/2025/Fundacao para a Ciencia e Tecnologia/ GR - LA/P/0050/2020/Fundacao para a Ciencia e Tecnologia/ GR - POCI-01-0145-FEDER-022122/Fundacao para a Ciencia e Tecnologia/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703393 OTO - NOTNLM OT - DJ-1 OT - Parkinson's disease OT - locus coeruleus OT - noradrenergic degeneration COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:34 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - cimb48101047 [pii] AID - cimb-48-01047 [pii] AID - 10.3390/cimb48101047 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 10;48(10):1047. doi: 10.3390/cimb48101047. PMID- 42939578 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 8 TI - Laminin-Derived Peptides and Isoforms: A Multifaceted Review of Their Roles in Cancer, Neurodegeneration, Inflammation, Tissue Regeneration, and Exercise Physiology. LID - 10.3390/cimb48101040 [doi] LID - 1040 AB - BACKGROUND: Laminins are heterotrimeric basement membrane glycoproteins whose chain composition, molecular processing, receptor engagement, and cellular source determine their biological effects. METHODS: We conducted an integrative narrative search of PubMed and manually screened reference lists for studies of laminin isoforms, laminin-derived peptides, and polylaminin in cancer, neurological disorders, inflammation, tissue regeneration, wound healing, and exercise-related adaptation. RESULTS: The evidence is heterogeneous and is predominantly mechanistic, in vitro, or preclinical. Laminin isoforms and derived peptides can produce opposing effects depending on the isoform or sequence, receptor, presentation format, tissue context, disease stage, and experimental model. Polylaminin is an experimental biomaterial with encouraging but preparation-dependent preclinical activity in selected neural repair models and in vitro stem cell culture. CONCLUSIONS: Laminin-based interventions should be developed in an indication-specific manner and require mechanistic validation, controlled delivery, dose-response characterization, safety assessment, reproducible manufacturing, and clinical validation before therapeutic translation. FAU - Silva, Elcirley Luz AU - Silva EL AD - World ParajiuJitsu Federation, Wilmington, DE 19805, USA. FAU - Kocerginsky, Gilberto AU - Kocerginsky G AD - Gilberto Kocerginsky Institute of Medicine and Health, Curitiba 80430-180, Parana, Brazil. FAU - Andrade, Claudia Marlise Balbinotti AU - Andrade CMB AUID- ORCID: 0000-0003-2765-3717 AD - Department of Basic Health Sciences, Faculty of Medicine, Cuiaba Campus, Federal University of Mato Grosso, Cuiaba 78060-900, Mato Grosso, Brazil. FAU - Silva, Romeu Paulo Martins AU - Silva RPM AUID- ORCID: 0000-0002-8368-158X AD - Center for Health Sciences, Postgraduate Program in Nutrition and Health, Maruipe Campus, Federal University of Espirito Santo, Vitoria 29075-910, Espirito Santo, Brazil. FAU - Magalhaes-Neto, Anibal Monteiro de AU - Magalhaes-Neto AM AD - Biologial and Health Science Institute, Araguaia Campus, Federal University of Mato Grosso, Pontal do Araguaia 78698-000, Mato Grosso, Brazil. FAU - Goncalves, Luis Carlos Oliveira AU - Goncalves LCO AD - Institute of Biotechnology, Laboratory of Biochemistry and Molecular Biology (LABIBI), Umuarama Campus, Federal University of Uberlandia, Uberlandia 38408-100, Minas Gerais, Brazil. LA - eng PT - Journal Article PT - Review DEP - 20261008 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703867 OTO - NOTNLM OT - AG73 OT - C16 OT - IKVAV OT - YIGSR OT - laminin gamma2 OT - laminin-111 OT - laminin-332 OT - laminin-511 OT - polylaminin COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:34 PHST- 2026/09/01 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - cimb48101040 [pii] AID - cimb-48-01040 [pii] AID - 10.3390/cimb48101040 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 8;48(10):1040. doi: 10.3390/cimb48101040. PMID- 42939577 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 7 TI - Integrative Single-Cell Transcriptomic Analysis Identifies Candidate Endothelial Activation Signatures in Atopic Dermatitis. LID - 10.3390/cimb48101039 [doi] LID - 1039 AB - Background: Endothelial activation is a recognized histological feature of atopic dermatitis (AD), but its transcriptional programs remain incompletely characterized. Methods: We reanalyzed public single-cell RNA-seq data from the skin of five patients with AD and four healthy donors using AD-associated gene set scoring, CellChat, endothelial hdWGCNA and pseudotime ordering, and examined candidate genes with eight machine learning classifiers and SHAP in public bulk transcriptomes of lesional and non-lesional AD skin. Results: Inferred intercellular interactions were more numerous in AD (1348 vs. 1111), with COLLAGEN, CD99 and MIF showing the largest gains in communication strength. Differences in AD-associated gene set scores between AD and healthy endothelial cells were small and inconsistent between scoring approaches. Among seven endothelial modules, Endo7, an NF-kappaB-responsive immediate-early module containing SELE, ICAM1, IRF1 and NFKBIA, overlapped most strongly with the AD gene set (10 genes; approximately 30-fold enrichment). AD endothelial cells were concentrated at intermediate pseudotime positions (Cohen's d = 0.334). In bulk skin, classifiers based on 14 LASSO-selected genes separated lesional from non-lesional AD skin in a small test set (AUC 0.70-0.98; likely optimistic estimates, as feature selection preceded the training-test split and paired samples from the same patients may span both partitions), with SELE, TOP2A and IRF1 ranked highest by SHAP. Conclusions: This hypothesis-generating analysis identifies AD-associated endothelial activation signatures and candidate genes that require confirmation in independent, clinically characterized cohorts and experimental studies. FAU - Cao, Fang AU - Cao F AUID- ORCID: 0000-0001-6506-9015 AD - School of Clinical Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China. FAU - Zhang, Hui AU - Zhang H AD - School of Clinical Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China. FAU - Guo, Cui AU - Guo C AD - School of Clinical Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China. FAU - Guo, Jing AU - Guo J AD - School of Clinical Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 610072, China. LA - eng GR - 82575065/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261007 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703613 OTO - NOTNLM OT - SHAP OT - atopic dermatitis OT - endothelial cells OT - hdWGCNA OT - machine learning OT - pseudotime trajectory COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/07 CRDT- 2026/10/27 10:34 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:34 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - cimb48101039 [pii] AID - cimb-48-01039 [pii] AID - 10.3390/cimb48101039 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 7;48(10):1039. doi: 10.3390/cimb48101039. PMID- 42939575 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 6 TI - Engineering the Biological Identity of Poly(lactic-co-glycolic Acid) Nanocarriers: Protein-Corona Dynamics, Functional Reprogramming, and Translational Fate. LID - 10.3390/cimb48101037 [doi] LID - 1037 AB - Poly(lactic-co-glycolic acid) (PLGA) nanocarriers can be engineered by varying polymer architecture, particle dimensions, surface chemistry, ligands, coatings, and cargo; however, biological exposure subsequently remodels the interface encountered by cells and tissues. This narrative review examines how this acquired biological identity may help explain translational failure between favorable formulation-level or in vitro properties and downstream in vivo performance. Across experimental and preclinical studies, serum or tissue-fluid composition, competitive protein exchange, physiological flow, complement and immunoglobulin recognition, ligand masking, tissue sequestration, and altered protein accessibility can redirect uptake, clearance, barrier interaction, biodistribution, efficacy, and safety. Corona formation, however, is not uniformly detrimental: selected interfaces can support dendritic-cell activation, blood-cell hitchhiking, or endogenous apolipoprotein-mediated targeting. We organize the literature as a biological stress-test continuum extending from the engineered pre-corona state through the acquired interface, cellular function, tissue transport, and whole-organism fate, while explicitly distinguishing direct PLGA evidence, mechanistically supported PLGA findings, associative PLGA observations, and comparator-only evidence. No clinical data or meta-analysis are included. The central translational implication is that PLGA performance should be judged by the robustness of its intended function after biologically relevant reprogramming rather than by pristine-particle properties alone. FAU - Omidian, Hossein AU - Omidian H AUID- ORCID: 0000-0002-5637-8729 AD - Department of Pharmaceutical Sciences, Barry and Judy Silverman College of Pharmacy, Nova Southeastern University, Fort Lauderdale, FL 33328, USA. FAU - Kianinejad, Nazanin AU - Kianinejad N AUID- ORCID: 0009-0006-0746-5611 AD - Gregory School of Pharmacy, Palm Beach Atlantic University, West Palm Beach, FL 33401, USA. FAU - Cubeddu, Luigi X AU - Cubeddu LX AUID- ORCID: 0000-0002-0154-6342 AD - Department of Pharmaceutical Sciences, Barry and Judy Silverman College of Pharmacy, Nova Southeastern University, Fort Lauderdale, FL 33328, USA. LA - eng PT - Journal Article PT - Review DEP - 20261006 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703797 OTO - NOTNLM OT - PLGA nanocarriers OT - bio-nano interface OT - biological identity OT - protein corona OT - translational nanomedicine COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/06 CRDT- 2026/10/27 10:33 PHST- 2026/09/11 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/06 00:00 [pmc-release] AID - cimb48101037 [pii] AID - cimb-48-01037 [pii] AID - 10.3390/cimb48101037 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 6;48(10):1037. doi: 10.3390/cimb48101037. PMID- 42939571 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 5 TI - Paired Transcriptomic Profiling Identifies Shared Liver Metastasis-Associated Molecular Features in Colorectal Cancer. LID - 10.3390/cimb48101033 [doi] LID - 1033 AB - Colorectal cancer liver metastasis (CCLM) is closely associated with poor patient prognosis, yet patient-specific molecular heterogeneity complicates the identification of recurrent molecular changes during metastasis. To address this, we performed RNA sequencing on paired primary tumors and liver metastases synchronously resected from six patients and analyzed gene expression changes using a paired design that accounted for interpatient variability. This analysis identified differentially expressed genes (DEGs) and metastasis-associated pathways through functional enrichment analysis and gene set enrichment analysis (GSEA). The reproducibility of these changes was further assessed across multiple independent publicly available transcriptomic datasets from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA). Selected candidate genes were additionally evaluated in a metastasis-selected colorectal cancer cell model, in which several genes showed expression changes consistent with the transcriptomic findings. Together, these results indicate that recurrent molecular changes accompany CCLM despite substantial interpatient heterogeneity and provide a strategy for identifying metastasis-associated molecular features and potential therapeutic targets. FAU - Lee, Hye Won AU - Lee HW AD - Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Department of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. FAU - Park, Seok Soon AU - Park SS AUID- ORCID: 0009-0001-6065-4845 AD - Medical Research Institute, Kangbuk Samsung Hospital, Seoul 03181, Republic of Korea. FAU - Kwon, Mi Ri AU - Kwon MR AD - Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Asan Preclinical Evaluation Center for Cancer Therapeutix, Asan Medical Center, Seoul 05505, Republic of Korea. FAU - Ju, Eun Jin AU - Ju EJ AD - Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Asan Preclinical Evaluation Center for Cancer Therapeutix, Asan Medical Center, Seoul 05505, Republic of Korea. FAU - Shin, Seol Hwa AU - Shin SH AD - Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Asan Preclinical Evaluation Center for Cancer Therapeutix, Asan Medical Center, Seoul 05505, Republic of Korea. FAU - Park, Jin AU - Park J AD - Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Asan Preclinical Evaluation Center for Cancer Therapeutix, Asan Medical Center, Seoul 05505, Republic of Korea. FAU - Ko, Eun Jung AU - Ko EJ AD - Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Asan Preclinical Evaluation Center for Cancer Therapeutix, Asan Medical Center, Seoul 05505, Republic of Korea. FAU - Son, Ga Won AU - Son GW AD - Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Department of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. FAU - Yang, Heetaek AU - Yang H AUID- ORCID: 0009-0001-7165-1092 AD - Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Department of Convergence Medicine, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. FAU - Park, Eun Jung AU - Park EJ AUID- ORCID: 0000-0002-4559-2690 AD - Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Division of Colon and Rectal Surgery, Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. FAU - Myung, Seung-Jae AU - Myung SJ AD - Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Department of Gastroenterology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. FAU - Kim, Chan Wook AU - Kim CW AD - Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Division of Colon and Rectal Surgery, Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. FAU - Lee, Jong Lyul AU - Lee JL AUID- ORCID: 0000-0002-5878-8000 AD - Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Division of Colon and Rectal Surgery, Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. FAU - Hwang, Jung Jin AU - Hwang JJ AUID- ORCID: 0000-0003-0912-1055 AD - Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Asan Preclinical Evaluation Center for Cancer Therapeutix, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. FAU - Lee, Shin-Wha AU - Lee SW AUID- ORCID: 0000-0002-5088-1905 AD - Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Asan Preclinical Evaluation Center for Cancer Therapeutix, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Department of Obstetrics and Gynecology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. FAU - Kim, Tae Won AU - Kim TW AD - Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Asan Preclinical Evaluation Center for Cancer Therapeutix, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Department of Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. FAU - Jeong, Seong-Yun AU - Jeong SY AUID- ORCID: 0000-0003-0959-5488 AD - Asan Institute for Life Sciences, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Asan Preclinical Evaluation Center for Cancer Therapeutix, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Department of Convergence Medicine, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. FAU - Song, Si Yeol AU - Song SY AUID- ORCID: 0000-0003-0090-8345 AD - Asan Preclinical Evaluation Center for Cancer Therapeutix, Asan Medical Center, Seoul 05505, Republic of Korea. AD - Department of Radiation Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea. LA - eng GR - RS-2023-00261982/Korea Health Industry Development Institute/ GR - U01 IP000086/IP/NCIRD CDC HHS/United States GR - RS-2023-00227084/National Research Foundation of Korea/ GR - RS-2024-00358981/National Research Foundation of Korea/ GR - 2024IP0086/Asan Medical Center/ PT - Journal Article DEP - 20261005 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703309 OTO - NOTNLM OT - Colorectal cancer OT - liver metastasis OT - metastasis-associated genes OT - tumor heterogeneity COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/05 CRDT- 2026/10/27 10:33 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/05 00:00 [pmc-release] AID - cimb48101033 [pii] AID - cimb-48-01033 [pii] AID - 10.3390/cimb48101033 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 5;48(10):1033. doi: 10.3390/cimb48101033. PMID- 42939569 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 4 TI - Exosomes as Molecular Mediators of Reproductive Health: From Infertility to HPV-Related Disease. LID - 10.3390/cimb48101031 [doi] LID - 1031 AB - This review provides a comprehensive overview of the biological roles of exosomes in reproductive physiology, examines their involvement in mechanisms associated with female and male infertility, and discusses their emerging role in human papillomavirus (HPV)-related reproductive disease. Exosomes are small extracellular vesicles that mediate intercellular communication by transporting proteins, lipids, DNA, messenger RNAs (mRNAs), microRNAs (miRNAs), long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), and other bioactive molecules, thereby regulating the biological functions of recipient cells. Particular emphasis is placed on the roles of exosomes in folliculogenesis, gametogenesis, fertilization, embryo implantation, and spermatogenesis. Alterations in exosomal cargo and function have been reported in several female and male reproductive disorders. In parallel, emerging evidence suggests that exosomes may participate in HPV-associated disease through mechanisms involving viral persistence, modulation of immune and inflammatory responses, and transfer of viral and host-derived molecules between cells. Exosomes are also being investigated as potential non-invasive biomarkers and therapeutic delivery platforms; however, these applications remain largely investigational. Importantly, substantial heterogeneity in extracellular vesicle isolation, purification, and characterization methods limits cross-study comparability and remains a major barrier to clinical translation. Overall, current evidence supports exosome-mediated communication as a biologically relevant mechanism across reproductive physiology, infertility-related disorders, and HPV-associated disease, while further standardized and clinically validated studies are required to establish their diagnostic and therapeutic utility. FAU - Siourtou, Serafoula-Natalia AU - Siourtou SN AD - Medical School, National and Kapodistrian University of Athens, 11528 Athens, Greece. FAU - Stavros, Sofoklis AU - Stavros S AD - Third Department of Obstetrics and Gynecology, University General Hospital "ATTIKON", Medical School, National and Kapodistrian University of Athens, 12462 Athens, Greece. FAU - Daskalaki, Maria-Anastasia AU - Daskalaki MA AD - First Department of Obstetrics and Gynecology, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 11528 Athens, Greece. FAU - Kokkosi, Eriketi AU - Kokkosi E AUID- ORCID: 0009-0007-5382-2170 AD - Department of Midwifery, Faculty of Health and Caring Sciences, University of West Attica, 12243 Athens, Greece. FAU - Zikopoulos, Athanasios AU - Zikopoulos A AUID- ORCID: 0009-0002-9553-6803 AD - Torbay and South Devon NHS Foundation Trust Lowes Brg, Torquay TQ2 7AA, UK. FAU - Mavrogianni, Despoina AU - Mavrogianni D AUID- ORCID: 0000-0003-1220-6594 AD - First Department of Obstetrics and Gynecology, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 11528 Athens, Greece. FAU - Kalogerakou, Vasileia AU - Kalogerakou V AD - Medical School, National and Kapodistrian University of Athens, 11528 Athens, Greece. FAU - Karampitsakos, Theodoros AU - Karampitsakos T AUID- ORCID: 0000-0002-4829-9190 AD - Third Department of Obstetrics and Gynecology, University General Hospital "ATTIKON", Medical School, National and Kapodistrian University of Athens, 12462 Athens, Greece. FAU - Moustakli, Efthalia AU - Moustakli E AUID- ORCID: 0009-0005-3333-2620 AD - Department of Nursing, School of Health Sciences, University of Ioannina, 45500 Ioannina, Greece. FAU - Arkouli, Nefeli AU - Arkouli N AD - Department of Obstetrics and Gynecology, Tzaneio General Hospital, 18536 Piraeus, Greece. FAU - Potiris, Anastasios AU - Potiris A AUID- ORCID: 0000-0001-7716-1521 AD - Third Department of Obstetrics and Gynecology, University General Hospital "ATTIKON", Medical School, National and Kapodistrian University of Athens, 12462 Athens, Greece. FAU - Daskalakis, Georgios AU - Daskalakis G AUID- ORCID: 0000-0001-7108-211X AD - First Department of Obstetrics and Gynecology, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 11528 Athens, Greece. FAU - Drakakis, Peter AU - Drakakis P AUID- ORCID: 0000-0002-8923-0333 AD - Third Department of Obstetrics and Gynecology, University General Hospital "ATTIKON", Medical School, National and Kapodistrian University of Athens, 12462 Athens, Greece. LA - eng PT - Journal Article PT - Review DEP - 20261004 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703754 OTO - NOTNLM OT - exosomes OT - extracellular vesicles OT - human papillomavirus (HPV) OT - precision medicine OT - reproductive medicine COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/04 CRDT- 2026/10/27 10:33 PHST- 2026/07/21 00:00 [received] PHST- 2026/09/03 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/04 00:00 [pmc-release] AID - cimb48101031 [pii] AID - cimb-48-01031 [pii] AID - 10.3390/cimb48101031 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 4;48(10):1031. doi: 10.3390/cimb48101031. PMID- 42939560 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 2 TI - SCN1A-Related Epilepsies: Clinical Spectrum and Mechanisms. LID - 10.3390/cimb48101023 [doi] LID - 1023 AB - Pathogenic variants in SCN1A are associated with a broad and overlapping spectrum of epilepsies, ranging from febrile seizures and genetic epilepsy with febrile seizures plus (GEFS+) to severe developmental and epileptic encephalopathies (DEEs), most notably Dravet syndrome. SCN1A encodes the voltage-gated sodium channel alpha-subunit Nav1.1, which has a critical role in inhibitory interneurons. In classical loss-of-function-associated Dravet syndrome, reduced Nav1.1 function contributes to impaired inhibitory neuronal excitability and network hyperexcitability. However, SCN1A-related disorders are mechanistically heterogeneous, with gain-of-function or mixed-function pathogenic variants being associated with clinically distinct phenotypes, including early-onset DEE and familial hemiplegic migraine type 3 (FHM3). This narrative review integrates functional variant classification with genotype-function-phenotype relationships, clinical diagnosis, and phenotype-specific treatment considerations. Particular emphasis is placed on early molecular testing, careful variant interpretation, and the distinction between chronic sodium-channel-blocker avoidance in classical loss-of-function-associated Dravet syndrome and potential mechanism-informed treatment strategies for selected gain-of-function phenotypes. We also summarize emerging SCN1A-targeted approaches, including antisense oligonucleotide therapy, gene activation, and interneuron-targeted gene replacement. A clearer understanding of variant-specific mechanisms and longitudinal clinical features may improve diagnostic precision, individualized management, and the development of disease-modifying therapies for SCN1A-related epilepsies. FAU - Moon, Ja-Un AU - Moon JU AD - Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea. FAU - Eom, Tae-Hoon AU - Eom TH AD - Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20261002 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703110 OTO - NOTNLM OT - Dravet syndrome OT - GABAergic interneuron OT - Nav1.1 OT - SCN1A OT - developmental and epileptic encephalopathy (DEE) OT - genetic diagnosis OT - genetic epilepsy with febrile seizures plus (GEFS+) OT - genotype-phenotype correlation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/02 CRDT- 2026/10/27 10:33 PHST- 2026/08/12 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/02 00:00 [pmc-release] AID - cimb48101023 [pii] AID - cimb-48-01023 [pii] AID - 10.3390/cimb48101023 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 2;48(10):1023. doi: 10.3390/cimb48101023. PMID- 42939557 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 2 TI - From Biological Activity to Precision Cannabinoid Oncology. LID - 10.3390/cimb48101019 [doi] LID - 1019 AB - Cannabinoids have gained increasing attention as potential anticancer agents because of their ability to modulate diverse cancer-associated signaling pathways and biological processes.: Cannabinoids, including cannabidiol (CBD) and Delta9-tetrahydrocannabinol (THC), can influence tumor proliferation, apoptosis, angiogenesis, metabolism, immune regulation, and tumor-microenvironment interactions. However, clinical translation remains limited, as evidence of anticancer efficacy is derived predominantly from preclinical studies. Major knowledge gaps include the absence of validated predictive biomarkers, unclear criteria for patient selection, and limited evidence regarding optimal therapeutic and combination strategies. Thus, the central challenge is to identify the patients and tumor contexts most likely to benefit from cannabinoid-based therapy. We propose a precision cannabinoid oncology framework that integrates molecular characterization, molecular classification, predictive biomarker development, rational combination strategies, and prospective clinical validation. This approach may provide a conceptual foundation for moving cannabinoid oncology from broadly empirical investigation toward molecularly informed patient selection and prospective validation of cannabinoid-based therapies in molecularly defined patient populations. FAU - Lee, Won Sup AU - Lee WS AUID- ORCID: 0000-0003-0863-1424 AD - Department of Internal Medicine, Institute of Medical Science, Gyeongsang National University Hospital, Gyeongsang National University College of Medicine, 15 Jinju-daero 816 Beon-gil, Jinju 52727, Republic of Korea. FAU - Go, Se-Il AU - Go SI AD - Department of Internal Medicine, Institute of Medical Science, Gyeongsang National University Changwon Hospital, Gyeongsang National University College of Medicine, 11 Samjeongja-ro, Changwon 51472, Republic of Korea. LA - eng PT - Journal Article DEP - 20261002 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703793 OTO - NOTNLM OT - biomarkers OT - cancer therapy OT - cannabinoids OT - clinical translation OT - multi-target activity OT - precision oncology COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/02 CRDT- 2026/10/27 10:33 PHST- 2026/08/18 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/02 00:00 [pmc-release] AID - cimb48101019 [pii] AID - cimb-48-01019 [pii] AID - 10.3390/cimb48101019 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 2;48(10):1019. doi: 10.3390/cimb48101019. PMID- 42939548 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 1 TI - The Potential of Cytokinins as Innovative Components of Multifunctional Biomaterials: Mechanisms of Biological Activity and Potential in Tissue Regeneration-In Vitro Studies-Review. LID - 10.3390/cimb48101010 [doi] LID - 1010 AB - Phytohormones, specifically from the cytokinin family, represent a promising class of sustainable, non-immunogenic alternatives to animal-derived growth factors in skin tissue engineering. This review explores the innovative integration of selected cytokinins-namely kinetin (K), benzyladenine (BA), and traumatic acid (TA)-into biocompatible biomaterials, focusing on their distinct molecular mechanisms and regenerative capacities in human cell models. At the cellular level, these compounds comprehensively modulate cell fate, proliferation, and survival. Benzyladenine and its derivatives regulate cell cycle transitions, enhance D-type cyclin expression, and selectively inhibit phosphodiesterase-4 (PDE4), thereby suppressing tumor necrosis factor-alpha synthesis to deliver crucial intrinsic anti-inflammatory benefits. Concurrently, kinetin functions as an advanced molecular shield and potent free radical scavenger, delaying the morphological features of fibroblast senescence and protecting nuclear DNA against oxidative damage by preventing 8-oxo-dG formation. Furthermore, the plant wound hormone traumatic acid exhibits a dual biological activity; it selectively serves as a significant antioxidant that accelerates extracellular matrix remodeling by driving protein and collagen biosynthesis in normal skin fibroblasts, while shifting to a pro-oxidant, cytotoxic mechanism in cancerous cells. By merging these specialized anti-inflammatory, anti-aging, and matrix-stimulating pathways into biocompatible scaffolds or hydrogels, cytokinin-functionalized biomaterials eliminate the safety and contamination risks associated with mammalian cell products. Ultimately, this approach establishes a highly cost-effective, scalable, and multi-functional platform capable of driving advanced tissue repair, enhancing wound healing dynamics, and ensuring significant biocompatibility. FAU - Jablonska-Trypuc, Agata AU - Jablonska-Trypuc A AUID- ORCID: 0000-0002-7711-3017 AD - Department of Chemistry, Biology and Biotechnology, Faculty of Civil Engineering and Environmental Sciences, Bialystok University of Technology, Wiejska 45E Street, 15-351 Bialystok, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261001 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703551 OTO - NOTNLM OT - benzyladenine OT - biomaterials OT - cytokinins OT - human cells OT - in vitro OT - kinetin OT - regeneration OT - skin OT - traumatic acid COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:33 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/13 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - cimb48101010 [pii] AID - cimb-48-01010 [pii] AID - 10.3390/cimb48101010 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 1;48(10):1010. doi: 10.3390/cimb48101010. PMID- 42939547 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Oct 1 TI - Pan-Cancer Somatic Mutation Landscape and Clinical Prognostic Relevance of Core Circadian Clock Genes Across the TCGA Cohort. LID - 10.3390/cimb48101009 [doi] LID - 1009 AB - Disruption of circadian rhythms is an emerging hallmark of cancer, yet whether somatic alterations in the core clock genes are subject to neutral stochastic processes or adaptive selection remains poorly defined. While complete circadian abrogation can cause genome instability and checkpoint-induced lethality, partial impairment might provide a context-dependent selective advantage by uncoupling cellular metabolic demand from environmental cycles. The somatic mutational landscape, 3D structural mapping, and clinical relevance of the 16 core circadian clock genes were systematically characterized across 10,239 human tumors representing 33 cancer types from The Cancer Genome Atlas (TCGA). A total of 2503 somatic mutation events were identified across 1294 mutated tumor samples (12.6% of pan-cancer cohort). Missense alterations dominated the mutational spectrum (61.2%, 1532/2503), with no deviation from the pan-cancer The Multi-Center Mutation Calling in Multiple Cancers (MC3) background rate (Z = -0.78, p = 0.44). In contrast to catastrophic inactivation, the clock genes were subject to selection at the level of preserved gene architecture. The highest absolute mutational burdens were concentrated in TIMELESS and Period family members (PER1, PER2, PER3) with missense variants mostly restricted to Uterine Corpus Endometrial Carcinoma (UCEC), Skin Cutaneous Melanoma (SKCM) and Colon Adenocarcinoma (COAD). Structural mapping highlighted the non-random accumulation of missense variants in heterodimerization interfaces, including the Per-Arnt-Sim (PAS) and basic helix-loop-helix (bHLH) domains, and in silico prediction identified a high proportion of deleterious substitutions. In survival modeling, associations between clock mutations and favorable prognosis were largely attenuated after multivariate adjustment for tumor mutational burden, highlighting potential lineage-specific confounding. In conclusion, somatic alterations in the mammalian circadian clockwork are predominantly non-synonymous substitutions that preserve overall gene architecture while altering conserved regulatory interfaces. These computational findings provide exploratory genomic observations consistent with the 'functional tuning' hypothesis, and raise the possibility of neoplastic cells selectively rewiring rather than completely abrogating circadian clock properties during oncogenesis. FAU - Civelek, Ilkay AU - Civelek I AUID- ORCID: 0000-0002-8241-1596 AD - Department of Biotechnology, Nigde Omer Halisdemir University, Nigde 51240, Turkiye. LA - eng PT - Journal Article DEP - 20261001 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13702863 OTO - NOTNLM OT - TCGA OT - TIMELESS OT - circadian clock OT - pan-cancer analysis OT - somatic mutations OT - survival analysis OT - tumor mutational burden COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/01 CRDT- 2026/10/27 10:33 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/01 00:00 [pmc-release] AID - cimb48101009 [pii] AID - cimb-48-01009 [pii] AID - 10.3390/cimb48101009 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Oct 1;48(10):1009. doi: 10.3390/cimb48101009. PMID- 42939545 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Sep 30 TI - Structural Parasitology Meets Nanomedicine: Current Structural Evidence and Future Opportunities for Leishmania and Schistosoma Targets. LID - 10.3390/cimb48101007 [doi] LID - 1007 AB - Neglected tropical diseases (NTDs) caused by Leishmania and Schistosoma species continue to impose a significant health burden in endemic regions, largely due to limited therapeutic options, drug resistance, and incomplete understanding of parasite biology at the molecular level. A major restriction in the development of targeted interventions is the structural inaccessibility of parasite membrane-associated proteins, which are central to host invasion, nutrient transport, immune evasion, and survival. Recent advances in cryogenic electron microscopy (cryo-EM) have expanded the structural biology toolkit available for studying difficult parasite proteins, particularly large and membrane-associated macromolecular assemblies. However, experimentally resolved cryo-EM structures remain limited for many of the Leishmania and Schistosoma targets relevant to therapeutic and vaccine development. This review therefore distinguishes experimentally determined structures from partial structural information and computational models and examines how potential cryo-EM approaches may address unresolved structural questions. We discuss current structural knowledge of selected parasite targets, approaches for recombinant expression and membrane-protein stabilization, membrane-mimetic systems for structural characterization, and computational methods that complement experimental structural biology. We further examine how experimentally supported structural information, complemented by computational predictions where appropriate, could contribute to structure-guided drug discovery, vaccine design, and targeted nanomedicine. At present, however, the direct translation of parasite structural information into rational nanotherapeutic design remains limited. Key gaps include the scarcity of experimentally resolved parasite membrane protein structures, challenges in their expression and structural characterization, and limited integration of structural biology with nanoparticle engineering. Resolving these gaps may improve the prospects for structure-informed therapeutic and nanomedicine applications against leishmaniasis, schistosomiasis, and other NTDs. FAU - Awote, Olasunkanmi Kayode AU - Awote OK AUID- ORCID: 0000-0002-0727-3998 AD - Molecular Biophysics and Structural Biology Group, Department of Biochemistry, Faculty of Science, University of Johannesburg, Auckland Park, Johannesburg 2006, Gauteng, South Africa. AD - Drosophila Research, Drug Discovery, and Drug Delivery Group, Department of Biochemistry, Faculty of Science, Lagos State University, Ojo, Lagos PMB 0001, Lagos State, Nigeria. FAU - Onilude, Hammed Ayantola AU - Onilude HA AD - Advanced Research Centre, School of Engineering, University of Glasgow, Glasgow G12 8QQ, UK. FAU - Kappo, Abidemi Paul AU - Kappo AP AUID- ORCID: 0000-0003-2521-8957 AD - Molecular Biophysics and Structural Biology Group, Department of Biochemistry, Faculty of Science, University of Johannesburg, Auckland Park, Johannesburg 2006, Gauteng, South Africa. LA - eng PT - Journal Article PT - Review DEP - 20260930 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703577 OTO - NOTNLM OT - Leishmania OT - Schistosoma OT - cryo-EM OT - lipid nanoparticles OT - membrane proteins OT - nanomedicine OT - structural parasitology COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:33 PHST- 2026/09/08 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - cimb48101007 [pii] AID - cimb-48-01007 [pii] AID - 10.3390/cimb48101007 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Sep 30;48(10):1007. doi: 10.3390/cimb48101007. PMID- 42939543 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Sep 30 TI - JAK/STAT Signaling in Oral Lichen Planus: From Cytokine-Driven Immune Dysregulation to Barrier Dysfunction and Targeted Therapy. LID - 10.3390/cimb48101006 [doi] LID - 1006 AB - Oral lichen planus (OLP) is a chronic, immune-mediated condition in which the oral epithelium acts simultaneously as a target of cytotoxic injury and a driver of persistent mucosal inflammation. This narrative review examines Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling as a network of cell- and cytokine-dependent signaling modules rather than a single linear pathway. Human OLP tissues show elevated expression of JAK1, phosphorylated JAK1, and various STAT-family proteins across both epithelial and inflammatory compartments. Functional modules demonstrate that IFN-gamma-JAK1/JAK2-STAT1 sensitizes basal keratinocytes to cytotoxic attack, while JAK2/STAT3 regulates epithelial survival, tight-junction integrity, and E-cadherin/CD103-dependent intraepithelial lymphocyte retention. Additionally, metabolic and redox inputs converge on the STAT3-CCL5 axis to reinforce T-cell recruitment, with spatial analyses placing cytotoxic T cells directly at the epithelial interface. Although clinical responses to targeted JAK inhibitors (including JAK1, JAK1/2, JAK1/3, JAK3/TEC, and TYK2 agents) are encouraging, most current evidence remains observational. Because inhibitor selectivity is relative, therapeutic efficacy alone cannot identify the primary upstream cytokine driver. Advancing OLP management requires moving beyond broad pathway inhibition toward spatially resolved phospho-endotyping linked with standardized clinical phenotypes, mechanistic biomarkers, and targeted local therapies to enable a true precision treatment strategy. FAU - Polizzi, Alessandro AU - Polizzi A AUID- ORCID: 0000-0001-6717-8899 AD - Department of General Surgery and Surgical-Medical Specialties, School of Dentistry, University of Catania, 95123 Catania, Italy. FAU - Campagna, Roberto AU - Campagna R AUID- ORCID: 0000-0003-2607-2734 AD - Department for the Promotion of Human Science and Quality of Life, San Raffaele Roma University, Via di Val Cannuta, 247, 00166 Rome, Italy. FAU - Tossetta, Giovanni AU - Tossetta G AUID- ORCID: 0000-0001-7003-1230 AD - Department for the Promotion of Human Science and Quality of Life, San Raffaele Roma University, Via di Val Cannuta, 247, 00166 Rome, Italy. FAU - Pesce, Paolo AU - Pesce P AUID- ORCID: 0000-0001-8726-4145 AD - Department of Surgical Sciences and Integrated Diagnostics (DISC), University of Genoa, 16132 Genoa, Italy. FAU - Marmo, Giorgia Maria AU - Marmo GM AUID- ORCID: 0009-0004-7131-5883 AD - Department of General Surgery and Surgical-Medical Specialties, School of Dentistry, University of Catania, 95123 Catania, Italy. FAU - Mascitti, Marco AU - Mascitti M AUID- ORCID: 0000-0002-7304-3752 AD - Department of Clinical Specialistic and Dental Sciences, Marche Polytechnic University, 60126 Ancona, Italy. FAU - Isola, Gaetano AU - Isola G AUID- ORCID: 0000-0003-4267-6992 AD - Department of General Surgery and Surgical-Medical Specialties, School of Dentistry, University of Catania, 95123 Catania, Italy. LA - eng PT - Journal Article PT - Review DEP - 20260930 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703167 OTO - NOTNLM OT - JAK/STAT signaling OT - JAK1 OT - JAK2 OT - JAK3 OT - Janus kinase inhibitors OT - STAT1 OT - STAT3 OT - TYK2 OT - epithelial barrier OT - oral lichen planus COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:33 PHST- 2026/08/29 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - cimb48101006 [pii] AID - cimb-48-01006 [pii] AID - 10.3390/cimb48101006 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Sep 30;48(10):1006. doi: 10.3390/cimb48101006. PMID- 42939541 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Sep 29 TI - Preliminary Pilot Assessment of TSPAN32 Detectability in Peripheral Blood as a Potential Circulating Biomarker in Advanced-Stage Nasopharyngeal Carcinoma Patients Undergoing Chemoradiation. LID - 10.3390/cimb48101003 [doi] LID - 1003 AB - Nasopharyngeal carcinoma (NPC) is highly prevalent in Southeast Asia, including Indonesia, with most patients presenting at advanced clinical stages. Reliable, minimally invasive circulating biomarkers for monitoring treatment response are lacking. This study aimed to identify candidate blood-based transcriptomic biomarkers for advanced-stage NPC through integrative bioinformatic analysis and to evaluate the expression dynamics of a prioritised candidate gene, TSPAN32, in peripheral blood of patients undergoing chemoradiation. Differentially expressed genes (DEGs) were identified from the GEO microarray dataset GSE53819 (18 advanced-stage NPC tissues vs. 18 non-cancerous nasopharyngeal tissues) using GEO2R. Candidate DEGs were prioritised through protein-protein interaction (PPI) network analysis (STRING confidence >/= 0.9), functional enrichment analysis (GO via Enrichr; KEGG pathway via ShinyGO v0.82), and survival analysis (Kaplan-Meier). Overlapping DEGs were identified against a previously published blood-based NPC transcriptomic dataset with 11 early-stage NPC patients, 11 advanced-stage NPC patients, and 11 healthy donors. Analysis of GSE53819 identified 943 upregulated and 1503 downregulated DEGs (|log(2)FC| >/= 1; Padj < 0.05). Functional enrichment of upregulated DEGs was dominated by inflammatory response and cytokine activity pathways, while downregulated DEGs were enriched in B-cell activation and immunoglobulin receptor binding. Protein-protein interaction network construction (STRING confidence >/= 0.9) and hub gene identification using the MCC algorithm identified six candidate genes among the downregulated DEGs: ADRA2A, SELP, TSPAN32, PEAR1, P2RX1, and DGKG. Kaplan-Meier survival analysis demonstrated that lower expression of SELP (HR = 0.57; p = 0.00053), TSPAN32 (HR = 0.56; p = 0.00025), and P2RX1 (HR = 0.48; p = 1.2 x 10(-5)) was significantly associated with shorter overall survival in head and neck carcinoma, supporting TSPAN32 as the primary candidate for clinical validation. RT-qPCR examination demonstrated that TSPAN32 transcript abundance in peripheral blood was significantly upregulated following chemoradiation in nine of ten patients (mean Log(2)FC = 2.08 +/- SD 1.09, 95% CI 1.29-2.86; one-sample t-test t(9) = 6.01, p = 0.0002; Wilcoxon signed-rank test p = 0.002). Primer specificity for TSPAN32 and ACTB was confirmed by melt-curve analysis and in silico validation (Primer-BLAST, OligoAnalyzer, UCSC In-Silico PCR). RBC transfusion status did not significantly modulate the magnitude of TSPAN32 expression change (independent-samples t-test, t = 1.86, p = 0.101). This integrative study identifies TSPAN32 as a candidate circulating biomarker responsive to chemoradiation in advanced-stage NPC. These exploratory findings require confirmation in larger, prospective, longitudinal cohorts before clinical translation. FAU - Mulansari, Nadia Ayu AU - Mulansari NA AUID- ORCID: 0000-0002-2242-7641 AD - Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Central Jakarta 10430, Indonesia. AD - Hematology-Medical Oncology Division, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Central Jakarta 10430, Indonesia. FAU - Paramita, Rafika Indah AU - Paramita RI AUID- ORCID: 0000-0002-8166-4479 AD - Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia, Central Jakarta 10430, Indonesia. AD - Bioinformatics Core Facilities-The Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Central Jakarta 10430, Indonesia. FAU - Wanandi, Septelia Inawati AU - Wanandi SI AUID- ORCID: 0000-0002-7963-8853 AD - Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Central Jakarta 10430, Indonesia. AD - Molecular Biology and Proteomics Core Facilities-The Indonesian Medical Education and Research Institute, Faculty of Medicine, Universitas Indonesia, Central Jakarta 10430, Indonesia. FAU - Sudoyo, Aru Wisaksono AU - Sudoyo AW AD - Hematology-Medical Oncology Division, Department of Internal Medicine, Faculty of Medicine, Universitas Indonesia, Central Jakarta 10430, Indonesia. FAU - Yudharto, Marlinda Adham AU - Yudharto MA AD - Department of Ear, Nose, and Throat Clinic, Faculty of Medicine, Universitas Indonesia, Central Jakarta 10430, Indonesia. LA - eng PT - Journal Article DEP - 20260929 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703889 OTO - NOTNLM OT - TSPAN32 OT - bioinformatics OT - differentially expressed genes OT - molecular markers OT - nasopharyngeal carcinoma OT - transcriptomics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/29 CRDT- 2026/10/27 10:33 PHST- 2026/08/17 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/29 00:00 [pmc-release] AID - cimb48101003 [pii] AID - cimb-48-01003 [pii] AID - 10.3390/cimb48101003 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Sep 29;48(10):1003. doi: 10.3390/cimb48101003. PMID- 42939535 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Sep 27 TI - Phytochemical Diversity and Multi-Endpoint In Vitro Biological Evaluation of Sisymbrium irio L.: Chemical Radical-Scavenging, Antibacterial, alpha-Amylase-/alpha-Glucosidase-Inhibitory, Cytotoxic, and Apoptosis-Associated Activities. LID - 10.3390/cimb48100997 [doi] LID - 997 AB - Sisymbrium irio L. (Brassicaceae) is an indigenous wild edible plant widely distributed in Saudi Arabia and the Mediterranean region and traditionally used in Unani and folk medicine to treat respiratory, digestive, and inflammatory ailments. However, its phytochemical diversity and organ-specific pharmacological potential remain insufficiently characterized. This study evaluated the phytochemical constituents; total phenolic and flavonoid contents; and in vitro antioxidant, antibacterial, cytotoxic/antiproliferative, apoptosis-associated and in vitro alpha-amylase- and alpha-glucosidase-inhibitory activities of methanolic leaf and stem extracts (SILEs and SISEs, respectively). GC-MS profiling putatively identified 82 compounds in SILE and 85 in SISE, revealing distinct organ-specific profiles. SISE showed higher FCRC and TFC (88.12 +/- 1.15 and 45.18 +/- 1.28 mg/g, respectively) and greater DPPH and ABTS(+) radical-scavenging capacities. In contrast, SILE exhibited greater antibacterial activity, particularly against Staphylococcus aureus and Enterococcus faecalis, with MIC values as low as 6.25 microg/mL. Both extracts showed in vitro concentration-dependent cytotoxic/antiproliferative activity against MCF-7 and HepG2 cells, accompanied by changes in the expression of apoptosis-associated genes. These findings indicate apoptosis-associated transcriptional responses but do not establish functional apoptosis or tumor-selective/anticancer activity. SILE exhibited moderate in vitro alpha-amylase- and alpha-glucosidase-inhibitory activity (IC(50) = 26.25 microg/mL (95% CI: 18.53-37.19 microg/mL) and 53.94 microg/mL (95% CI: 47.05-61.83 microg/mL), respectively). Overall, this study provides the first integrated comparison of SILE and SISE across multiple in vitro bioactivities, highlighting distinct organ-specific pharmacological potential that requires more investigation. These results do not prove therapeutic efficacy and should be viewed as first in vitro screening findings. FAU - Aziz, Ibrahim M AU - Aziz IM AUID- ORCID: 0000-0002-6970-5207 AD - Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia. FAU - Alshalan, Rawan M AU - Alshalan RM AD - Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia. FAU - Abdulmanea, Adel A AU - Abdulmanea AA AD - Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia. FAU - Almuqrin, Abdulaziz M AU - Almuqrin AM AUID- ORCID: 0009-0004-7025-6543 AD - Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 12372, Saudi Arabia. FAU - Alghamdi, Amal Khalaf AU - Alghamdi AK AD - Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia. LA - eng GR - ORF-2026-1520/King Saud University/ PT - Journal Article DEP - 20260927 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703188 OTO - NOTNLM OT - Sisymbrium irio L. OT - antibacterial activity OT - apoptosis-associated gene expression OT - chemical radical-scavenging activity OT - cytotoxicity OT - natural products OT - phytochemicals OT - alpha-amylase inhibition OT - alpha-glucosidase inhibition COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/27 CRDT- 2026/10/27 10:33 PHST- 2026/08/30 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - cimb48100997 [pii] AID - cimb-48-00997 [pii] AID - 10.3390/cimb48100997 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Sep 27;48(10):997. doi: 10.3390/cimb48100997. PMID- 42939531 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Sep 25 TI - Targeting Glucose Metabolism in Bladder Cancer: Therapeutic Implications of 2-Deoxy-d-glucose. LID - 10.3390/cimb48100993 [doi] LID - 993 AB - Bladder cancer is characterized by metabolic reprogramming, and enhanced glycolysis contributes to tumor progression and therapeutic resistance. Because the anticancer activity of 2-Deoxy-d-glucose (2-DG) has been reported in several malignancies, this study evaluated the antiproliferative and metabolism-modulating effects of 2-DG and their association with oxidative stress-related signaling in bladder cancer models. Human bladder cancer cell lines were examined using MTT assays, hexokinase (HK) activity assays, ATP measurements, DCF-DA-based reactive oxygen species (ROS) analysis, and Western blotting. RT112 and 253J cells were selected for mechanistic analyses, and the antitumor efficacy of 2-DG was further evaluated in xenograft models. Treatment with 2-DG reduced the metabolic activity of all five bladder cancer cell lines in a concentration-dependent manner and was associated with decreased HK activity and intracellular ATP levels. Increased ROS-sensitive fluorescence was accompanied by upregulation of TXNIP and BAX and downregulation of AKT and BCL-2, indicating a shift toward a pro-apoptotic pattern of apoptosis-related protein expression. In vivo, systemic administration of 2-DG significantly suppressed xenograft tumor growth without apparent body weight loss or histological abnormalities in the liver or kidneys. These findings suggest that 2-DG suppresses bladder cancer growth in association with metabolic disruption, increased ROS-sensitive fluorescence, TXNIP/AKT-related changes, and modulation of BAX/BCL-2 expression. However, additional studies are required to clarify the underlying mechanisms and further evaluate its safety profile. FAU - Zhang, Xia AU - Zhang X AD - Department of Urology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho 761-0793, Kita-gun, Kagawa, Japan. FAU - Taoka, Rikiya AU - Taoka R AUID- ORCID: 0000-0003-3243-499X AD - Department of Urology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho 761-0793, Kita-gun, Kagawa, Japan. FAU - Liu, Dage AU - Liu D AD - Department of General Thoracic Surgery, Faculty of Medicine, 1750-1 Ikenobe, Miki-cho 761-0793, Kita-gun, Kagawa, Japan. FAU - Hossain, Akram AU - Hossain A AD - Department of Urology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho 761-0793, Kita-gun, Kagawa, Japan. FAU - Matsuoka, Yuki AU - Matsuoka Y AD - Department of Urology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho 761-0793, Kita-gun, Kagawa, Japan. FAU - Tohi, Yoichiro AU - Tohi Y AUID- ORCID: 0000-0003-3884-8915 AD - Department of Urology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho 761-0793, Kita-gun, Kagawa, Japan. FAU - Naito, Hirohito AU - Naito H AD - Department of Urology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho 761-0793, Kita-gun, Kagawa, Japan. FAU - Akimitsu, Kazuya AU - Akimitsu K AD - International Institute of Rare Sugar Research and Education, Kagawa University, 2393 Ikenobe, Miki-cho 761-0795, Kita-gun, Kagawa, Japan. FAU - Izumori, Ken AU - Izumori K AD - International Institute of Rare Sugar Research and Education, Kagawa University, 2393 Ikenobe, Miki-cho 761-0795, Kita-gun, Kagawa, Japan. FAU - Sugimoto, Mikio AU - Sugimoto M AD - Department of Urology, Faculty of Medicine, Kagawa University, 1750-1 Ikenobe, Miki-cho 761-0793, Kita-gun, Kagawa, Japan. LA - eng PT - Journal Article DEP - 20260925 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703226 OTO - NOTNLM OT - 2-Deoxy-d-glucose OT - AKT OT - TXNIP OT - apoptosis OT - bladder cancer OT - glycolysis OT - hexokinase OT - oxidative stress OT - xenograft model COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/25 CRDT- 2026/10/27 10:33 PHST- 2026/08/07 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - cimb48100993 [pii] AID - cimb-48-00993 [pii] AID - 10.3390/cimb48100993 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Sep 25;48(10):993. doi: 10.3390/cimb48100993. PMID- 42939522 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Sep 24 TI - Melatonin Differentially Modulates Temozolomide Responses in Glioblastoma and Endothelial Cells: Associations with Global DNA Methylation and Cortistatin Levels. LID - 10.3390/cimb48100984 [doi] LID - 984 AB - Glioblastoma (GBM) is the most aggressive primary brain tumor in adults and remains highly resistant to current therapeutic strategies. Temozolomide (TMZ) is a standard chemotherapeutic agent used in GBM treatment; however, intrinsic and acquired resistance limits its efficacy. This study investigated treatment-associated response patterns to TMZ and melatonin in U87MG glioblastoma cells and human umbilical vein endothelial cells (HUVECs). Relative MTT metabolic activity was assessed using the MTT assay, and cell-cycle distribution was analyzed by flow cytometry. Global DNA methylation was evaluated using a 5-methylcytosine assay, with calibration-derived values expressed as %5-mC. CORT was assessed semi-quantitatively by ELISA, with background-corrected OD values normalized to the corresponding untreated control within each experimental run and expressed as relative CORT levels (% of control). Linear mixed-effects models were used to evaluate TMZ concentration, melatonin exposure, and the TMZ x melatonin interaction, with biological experiment included as a random effect. TMZ and melatonin produced treatment-condition- and cell-type-associated response patterns. In HUVECs, relative MTT metabolic activity remained comparatively high across most TMZ concentrations, whereas a more pronounced decrease was observed at 100 microM TMZ during melatonin co-treatment. U87MG cells showed a non-linear MTT response across TMZ concentrations, and the magnitude and direction of the melatonin-associated effect varied according to TMZ dose. Significant TMZ x melatonin interactions were identified for relative MTT metabolic activity in both cell types. Global DNA methylation-related measurements also showed treatment-associated differences, with distinct response patterns between HUVEC and U87MG cells; calibration-derived %5-mC values were used for quantitative presentation of global DNA methylation. Relative CORT levels likewise varied across treatment conditions, with larger relative changes observed in U87MG cells under several combination conditions. The observed findings should be interpreted as exploratory and hypothesis-generating. The present data do not establish therapeutic selectivity, pharmacological synergy, a causal epigenetic mechanism, or a CORT-mediated stress-response pathway. Further validation using additional glioblastoma models, non-malignant neural controls, fully quantitative and appropriately normalized CORT measurements, and targeted functional pathway analyses is required before mechanistic or clinical conclusions can be drawn. FAU - Gundogan, Gul Ipek AU - Gundogan GI AUID- ORCID: 0000-0002-9438-6113 AD - Department of Histology and Embryology, Faculty of Medicine, Biruni University, 34015 Istanbul, Turkiye. AD - Biruni Advanced Technology Application and Research Center (B@MER), Biruni University, 34015 Istanbul, Turkiye. FAU - Yurttas, Asiye Gok AU - Yurttas AG AUID- ORCID: 0000-0002-6424-7411 AD - Department of Biochemistry, Faculty of Medicine, Istanbul Atlas University, 34408 Istanbul, Turkiye. FAU - Oltulu, Yasemin Musteri AU - Oltulu YM AD - Biruni Advanced Technology Application and Research Center (B@MER), Biruni University, 34015 Istanbul, Turkiye. AD - Department of Medical Biology, Faculty of Medicine, Biruni University, 34015 Istanbul, Turkiye. FAU - Ciraci, Enver AU - Ciraci E AD - Biruni Advanced Technology Application and Research Center (B@MER), Biruni University, 34015 Istanbul, Turkiye. AD - Department of Biochemistry, Faculty of Pharmacy, Biruni University, 34015 Istanbul, Turkiye. FAU - Arslan, Halil Ibrahim AU - Arslan HI AUID- ORCID: 0009-0001-4172-4748 AD - Biruni Advanced Technology Application and Research Center (B@MER), Biruni University, 34015 Istanbul, Turkiye. FAU - Elgun, Tugba AU - Elgun T AUID- ORCID: 0000-0002-1311-6892 AD - Biruni Advanced Technology Application and Research Center (B@MER), Biruni University, 34015 Istanbul, Turkiye. AD - Department of Medical Biology, Faculty of Medicine, Biruni University, 34015 Istanbul, Turkiye. LA - eng PT - Journal Article DEP - 20260924 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13702877 OTO - NOTNLM OT - CORT OT - HUVECs OT - U87MG cells OT - cell cycle OT - glioblastoma OT - global DNA methylation OT - melatonin OT - relative MTT metabolic activity OT - temozolomide COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:33 PHST- 2026/08/14 00:00 [received] PHST- 2026/09/17 00:00 [revised] PHST- 2026/09/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - cimb48100984 [pii] AID - cimb-48-00984 [pii] AID - 10.3390/cimb48100984 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Sep 24;48(10):984. doi: 10.3390/cimb48100984. PMID- 42939520 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Sep 24 TI - Plasma LTA4H as an Inflammatory Target for Monitoring +Gz-Induced Injury in Rabbits. LID - 10.3390/cimb48100982 [doi] LID - 982 AB - Repeated +Gz exposure during high-performance flight can cause inflammatory injury, but whether LTA4H and its related molecules are altered after such exposure and whether LTA4H could serve as a plasma target for monitoring +Gz-induced inflammatory injury remain unclear. Eighteen male New Zealand white rabbits were assigned to control, 2-week, and 4-week groups (n = 6 per group). Plasma proteomics was performed using data-independent acquisition mass spectrometry. LTA4H, LTB4, and IL-6 were quantified by ELISA. Correlations among the three molecules were analyzed by Pearson correlation. Diagnostic performance was assessed by receiver operating characteristic curve analysis. Proteomics identified LTA4H as nominally upregulated in both the 2-week and 4-week groups, but it did not remain significant after false discovery rate correction. It was therefore selected as an exploratory candidate for orthogonal confirmation by ELISA. ELISA confirmed that plasma LTA4H, LTB4, and IL-6 were all significantly elevated after +Gz exposure. Positive correlations were observed in the pooled analysis, but these were attenuated after adjusting for group. LTA4H showed exploratory discrimination of exposure status, with an AUC exceeding 0.9. Repeated +Gz exposure was associated with concurrent elevation of LTA4H, LTB4, and IL-6. LTA4H may serve as a potential plasma target for monitoring +Gz-induced inflammatory injury. FAU - Wu, Yunchong AU - Wu Y AUID- ORCID: 0009-0002-7244-3073 AD - Naval Medical Center, Naval Medical University, Shanghai 200433, China. FAU - Luo, Tailai AU - Luo T AD - Naval Medical Center, Naval Medical University, Shanghai 200433, China. FAU - Zhai, Jia AU - Zhai J AD - Naval Medical Center, Naval Medical University, Shanghai 200433, China. FAU - Wang, Yihe AU - Wang Y AD - Naval Medical Center, Naval Medical University, Shanghai 200433, China. FAU - Wang, Lichao AU - Wang L AD - Naval Medical Center, Naval Medical University, Shanghai 200433, China. FAU - He, Yutao AU - He Y AUID- ORCID: 0009-0001-8566-6375 AD - Naval Medical Center, Naval Medical University, Shanghai 200433, China. FAU - Shen, Jun AU - Shen J AD - Naval Medical Center, Naval Medical University, Shanghai 200433, China. FAU - Yao, Yongjie AU - Yao Y AD - Naval Medical Center, Naval Medical University, Shanghai 200433, China. FAU - Zhu, Wei AU - Zhu W AD - Naval Medical Center, Naval Medical University, Shanghai 200433, China. LA - eng PT - Journal Article DEP - 20260924 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703552 OTO - NOTNLM OT - IL-6 OT - LTA4H OT - LTB4 OT - biomarker OT - hypergravity OT - inflammation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:33 PHST- 2026/08/10 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - cimb48100982 [pii] AID - cimb-48-00982 [pii] AID - 10.3390/cimb48100982 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Sep 24;48(10):982. doi: 10.3390/cimb48100982. PMID- 42939519 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Sep 24 TI - Emapalumab in IFN-gamma-Driven Hyperinflammatory Disorders: Mechanisms, Clinical Evidence, and Emerging Therapeutic Applications. LID - 10.3390/cimb48100981 [doi] LID - 981 AB - Hyperinflammatory disorders are severe clinical syndromes characterized by uncontrolled immune activation, sustained release of inflammatory mediators, and multiorgan injury. Hemophagocytic lymphohistiocytosis (HLH) and macrophage activation syndrome (MAS) are two representative hyperinflammatory syndromes. Growing evidence has identified interferon gamma (IFN-gamma) as an important mediator, contributing to persistent immune activation and amplification of inflammatory responses. Emapalumab is a fully human, high-affinity monoclonal antibody targeting IFN-gamma that exerts its therapeutic effects by selectively neutralizing IFN-gamma and suppressing its downstream inflammatory activity. Clinical studies have established the therapeutic utility of emapalumab in primary HLH, while its potential applications are increasingly being explored in Still's disease-associated MAS, secondary HLH, chimeric antigen receptor (CAR) T-cell therapy-associated hyperinflammatory states, and hematopoietic stem cell transplantation-related settings. This review summarizes the biological basis of IFN-gamma-driven hyperinflammation, the molecular mechanism of action of emapalumab, and current clinical evidence for its use in HLH, MAS, and other hyperinflammatory conditions, while discussing the limitations of existing evidence and priorities for future research. FAU - Qian, Yayi AU - Qian Y AD - Department of Hematology & Oncology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China. FAU - Xu, Xiaojun AU - Xu X AUID- ORCID: 0000-0003-1388-2535 AD - Department of Hematology & Oncology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China. LA - eng GR - 81970122/National Natural Science Foundation of China/ PT - Journal Article PT - Review DEP - 20260924 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13702908 OTO - NOTNLM OT - clinical trials OT - emapalumab OT - hemophagocytic lymphohistiocytosis OT - hyperinflammation OT - interferon-gamma OT - macrophage activation syndrome COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analysis, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:33 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - cimb48100981 [pii] AID - cimb-48-00981 [pii] AID - 10.3390/cimb48100981 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Sep 24;48(10):981. doi: 10.3390/cimb48100981. PMID- 42939517 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Sep 24 TI - Ovarian Microvascular Endothelial Cell Migration Is Dysregulated in PMOS. LID - 10.3390/cimb48100979 [doi] LID - 979 AB - The objective of this study is to investigate whether ovarian microvascular endothelial cells (OMECs) from women with polyendocrine metabolic ovarian syndrome (PMOMECs) exhibit altered angiogenic responses compared with cells from healthy oocyte donors (HOMECs). This is a laboratory-based cross-sectional study using HOMECs and PMOMECs obtained from discarded follicular aspirates. Expression of mRNA and protein for VEGF receptors (FLT1, KDR) and PGE2 receptors (PTGER1-4) were assessed using qPCR and immunofluorescence. OMECs were treated in vitro with VEGFA, PGE2, or receptor-selective agonists. Migration was quantified via porous membrane inserts and live cell tracking. Proliferation was measured via BrdU incorporation. FLT1 and KDR mRNA and protein levels were similar between HOMECs and PMOMECs. PTGER2 mRNA was higher and PTGER3 mRNA was lower in PMOMECs. VEGFA and PGE2 significantly stimulated migration and proliferation in HOMECs. In contrast, PMOMECs failed to exhibit increased migration in response to PGE2 or selective PTGER agonists, while VEGFA-induced migration remained comparable to migration of HOMECs. Live cell tracking revealed that PMOMEC migration resulted in decreased overall net distance traveled and reduced directional persistence (less linear movement) compared with HOMECs. These findings demonstrate that ovarian microvascular endothelial cells from women with PMOS display impaired angiogenic responses to PGE2, which may contribute to altered follicle growth and anovulation in PMOS. FAU - Yeshua, Arielle S AU - Yeshua AS AD - The Jones Institute, Eastern Virginia Medical School, Old Dominion University, Norfolk, VA 23507, USA. AD - Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Old Dominion University, Norfolk, VA 23507, USA. FAU - Sage, Megan A G AU - Sage MAG AD - Department of Biomedical and Translational Sciences, Eastern Virginia Medical School, Old Dominion University, Norfolk, VA 23507, USA. FAU - Duffy, Diane M AU - Duffy DM AUID- ORCID: 0000-0001-9345-2740 AD - Department of Biomedical and Translational Sciences, Eastern Virginia Medical School, Old Dominion University, Norfolk, VA 23507, USA. LA - eng GR - The Jones Family Reproductive Medicine Research Fund./ PT - Journal Article DEP - 20260924 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703554 OTO - NOTNLM OT - PTGER OT - VEGFR1 OT - VEGFR2 OT - angiogenesis OT - microvasculature OT - polycystic ovary syndrome OT - polyendocrine metabolic ovarian syndrome OT - prostaglandin E2 COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/24 CRDT- 2026/10/27 10:33 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - cimb48100979 [pii] AID - cimb-48-00979 [pii] AID - 10.3390/cimb48100979 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Sep 24;48(10):979. doi: 10.3390/cimb48100979. PMID- 42939516 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Sep 23 TI - Age-Dependent Remodeling of Parvalbumin Interneuron-Associated Networks Alters Corticospinal Output in the Motor Cortex. LID - 10.3390/cimb48100978 [doi] LID - 978 AB - The primary motor cortex (M1) controls voluntary movements through coordinated interactions between excitatory pyramidal neurons and inhibitory parvalbumin-expressing interneurons (PV-INs). Although PV-INs are critical to regulating motor output and motor coordination, their role in age-related motor decline remains unclear. Here, we investigated how aging alters PV-IN-associated regulation of corticospinal tract (CST) output in mice. Aged mice (14-24 months) exhibited selective motor impairments, including deficits in balance and hindlimb-supported performance, while general locomotor activity remained largely preserved. We combined optogenetics, ex vivo electrophysiology, retrograde tracing, and in vivo electromyographic (EMG) recordings. In aged M1 slices, optogenetic stimulation of the PV-IN-targeted network produced small extracellular-like voltage deflections recorded with a loose-apposed, non-sealed pipette configuration. These responses persisted during GABA(A) receptor blockade but were abolished by DNQX and APV, indicating engagement of an ionotropic glutamate receptor-dependent network mechanism. Whole-cell recordings obtained in separate experiments demonstrated that conventional inhibitory PV-IN-to-pyramidal transmission remained detectable during repetitive stimulation. In vivo, PV-IN activation increased M1-evoked hindlimb EMG responses in aged mice but had little effect in young animals. Together, these findings identify an age-dependent reorganization of PV-IN-associated motor-cortical network function that alters the recruitment of glutamatergic mechanisms and corticospinal motor output. The present data do not establish the precise synaptic topology of this network response or whether the reorganization is compensatory or maladaptive. FAU - Wei, Xiaofei AU - Wei X AUID- ORCID: 0000-0002-1173-4734 AD - Department of Neurosurgery, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, CA 90095, USA. AD - Department of Neurology, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, CA 90095, USA. AD - Department of Anatomy and Neurobiology, University of California-Irvine, Irvine, CA 92617, USA. FAU - Mody, Istvan AU - Mody I AD - Department of Neurology, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, CA 90095, USA. FAU - Wu, Yixin AU - Wu Y AD - Department of Neurosurgery, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, CA 90095, USA. FAU - Gajjar, Aryan AU - Gajjar A AD - Department of Neurosurgery, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, CA 90095, USA. FAU - Singh, Gurnoor AU - Singh G AD - Department of Neurosurgery, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, CA 90095, USA. FAU - Neerathalingam, Vignesh AU - Neerathalingam V AUID- ORCID: 0009-0008-5711-7900 AD - Department of Neurosurgery, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, CA 90095, USA. FAU - Sun, Adeline AU - Sun A AUID- ORCID: 0000-0001-8131-9352 AD - Department of Neurology, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, CA 90095, USA. FAU - Huang, Ruyi AU - Huang R AUID- ORCID: 0000-0003-3575-3793 AD - Department of Neurosurgery, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, CA 90095, USA. FAU - Holley, Sandra M AU - Holley SM AUID- ORCID: 0000-0002-2643-4961 AD - Department of Neurosurgery, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, CA 90095, USA. FAU - Lu, Daniel C AU - Lu DC AD - Department of Neurosurgery, David Geffen School of Medicine, University of California Los Angeles (UCLA), Los Angeles, CA 90095, USA. LA - eng GR - R01 DA047637/DA/NIDA NIH HHS/United States GR - 5R01DA047637-05/NH/NIH HHS/United States PT - Journal Article DEP - 20260923 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13702875 OTO - NOTNLM OT - aging OT - corticospinal tract OT - electromyography OT - optogenetics OT - parvalbumin interneuron OT - primary motor cortex COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/23 CRDT- 2026/10/27 10:33 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - cimb48100978 [pii] AID - cimb-48-00978 [pii] AID - 10.3390/cimb48100978 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Sep 23;48(10):978. doi: 10.3390/cimb48100978. PMID- 42939512 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Sep 22 TI - Deoxycholic Acid at the Interface of Bile Acid Metabolism and Adipose Tissue Remodeling: From Endogenous Signaling to Injectable Adipocytolysis. LID - 10.3390/cimb48100974 [doi] LID - 974 AB - Deoxycholic acid (DCA) occupies an unusual position at the boundary between endogenous metabolism and therapeutic tissue ablation. Under physiological conditions, DCA is a gut microbial secondary bile acid that enters the enterohepatic circulation, contributes to the composition of the bile-acid pool, and participates in receptor-dependent signaling. When delivered as a concentrated injectable drug into subcutaneous fat, however, DCA acts primarily as an amphipathic cytolytic molecule that disrupts cell membranes, initiates adipocyte death, and provokes an organized inflammatory and reparative response. These two biological identities are often conflated, leading to imprecise descriptions of injectable DCA as a lipolytic, receptor-targeted, or metabolically selective treatment. This critical review integrates evidence from bile-acid biochemistry, microbiology, membrane biophysics, adipose-tissue biology, human histology, pharmacokinetics, and clinical trials. We describe the microbial formation, transport, conjugation, and signaling actions of endogenous DCA; distinguish physiological receptor signaling from concentration-dependent detergent injury; and examine the temporal sequence of adipocytolysis, innate immune recruitment, lipid clearance, fibrovascular remodeling, and volume reduction after local injection. We also assess systemic exposure, clinical efficacy, anatomic determinants of safety, and the limitations of extrapolating submental data to large-volume or non-submental treatment. Finally, we propose a translational metabolomics and lipidomics framework that combines local interstitial sampling, plasma bile-acid profiling, lipid mediator analysis, imaging, and tissue phenotyping. The central conclusion is that injectable DCA is best understood as a controlled local cytolytic perturbation superimposed on a tightly regulated endogenous bile-acid system. Framing the intervention in this way clarifies terminology, identifies measurable biomarkers, and creates a rational path toward dose optimization, patient selection, and safer clinical translation. FAU - Yi, Kyu-Ho AU - Yi KH AUID- ORCID: 0000-0001-5572-1364 AD - Division in Anatomy and Developmental Biology, Department of Oral Biology, Human Identification Research Institute, College of Dentistry, Yonsei, 50-1 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea. AD - You and I Clinic, 470 Gangnam-daero, Seoul 04524, Republic of Korea. FAU - Lee, Han Earl AU - Lee HE AD - Re & Re Plastic Surgery Clinic, 423 Gangnam-daero, Seoul 04524, Republic of Korea. FAU - Kim, Hong Seok AU - Kim HS AUID- ORCID: 0009-0000-1156-7393 AD - VOS Dermatology Clinic, 806 Eonju-ro, Seoul 06214, Republic of Korea. FAU - Techapichetvanich, Thanya AU - Techapichetvanich T AD - Department of Dermatology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand. LA - eng PT - Journal Article PT - Review DEP - 20260922 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703840 OTO - NOTNLM OT - ATX-101 OT - FXR OT - TGR5 OT - adipocytolysis OT - adipose tissue OT - bile acids OT - deoxycholic acid OT - gut microbiome OT - immunometabolism OT - lipidomics OT - metabolomics OT - submental fat COIS- The authors declare no conflict of interest. K.-H.Y. is an employees of You and I Clinic, Seoul, Republic of Korea. H.E.L. is an employee of Re & Re Plastic Surgery Clinic, Seoul, Republic of Korea. H.S.K. is is an employee of VOS Dermatology Clinic, Seoul, Republic of Korea. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:33 PHST- 2026/07/17 00:00 [received] PHST- 2026/09/05 00:00 [revised] PHST- 2026/09/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - cimb48100974 [pii] AID - cimb-48-00974 [pii] AID - 10.3390/cimb48100974 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Sep 22;48(10):974. doi: 10.3390/cimb48100974. PMID- 42939511 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Sep 22 TI - Dissecting the Genetics of Forage Yield and Related Traits in Soft Red Winter Wheat. LID - 10.3390/cimb48100973 [doi] LID - 973 AB - The use of wheat as forage remains underutilized, particularly in the southeastern U.S., despite its potential to fill seasonal forage gaps when warm-season forages are dormant. Genetic improvement of forage yield traits in wheat has been limited. A genome-wide association study on 182 soft red winter wheat lines was conducted to identify quantitative trait loci for five forage yield traits. The panel was evaluated across four environmental-year combinations in Georgia (2023-2025). ANOVA revealed significant phenotypic variation, with heritability ranging from low to moderate in combined locations and from low to high within individual locations. Correlation analysis revealed significant positive associations between growth stages (GS), forage height (FHt), fresh forage weight (FWt), and dry forage weight (DWt). Using 47,339 SNP markers, we identified 192 marker-trait associations. Twenty-two major-effect QTLs explained 10% of the phenotypic variance, of which six were multi-trait. Chromosomes 7A, 1B, and 6B harbor key regions for FHt, FWt, and DWt. Accumulating favorable alleles showed significant increases in FHt (7 cm), FWt (1611.2 kg/ha), DWt (58.6 kg/ha), and germination counts (G) (4.5%), while reducing GS (0.81 units). QTLs (QFht.uga-7A, QFht.uga-4A) were possibly allelic with known loci, and the remaining 20 QTLs appeared novel. Candidate genes were identified for 10 of 22 QTLs related to plant growth and development. This study provides promising candidates for forage yield improvement in wheat under forage-production systems, supporting marker-assisted selection. Upon further validation, these results can be used in breeding for desirable plant architecture to improve wheat potential for forage yield. FAU - Ali, Maria AU - Ali M AD - Department of Crop & Soil Sciences, University of Georgia, Athens, GA 30602, USA. FAU - Missaoui, Ali AU - Missaoui A AUID- ORCID: 0000-0003-1710-1142 AD - Department of Crop & Soil Sciences, University of Georgia, Athens, GA 30602, USA. FAU - Subedi, Madhav AU - Subedi M AUID- ORCID: 0000-0001-8032-7641 AD - Institute of Biotechnology, Cornell University, Ithaca, NY 14853, USA. FAU - Sapkota, Suraj AU - Sapkota S AD - Small Grains and Potato Germplasm Research Unit, Aberdeen, ID 83210, USA. FAU - Babar, Ali AU - Babar A AUID- ORCID: 0000-0001-9951-6856 AD - Agronomy Department, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA. FAU - Mergoum, Mohamed AU - Mergoum M AD - Department of Crop & Soil Sciences, University of Georgia, Athens, GA 30602, USA. LA - eng PT - Journal Article DEP - 20260922 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703133 OTO - NOTNLM OT - GWAS OT - QTL OT - dual-purpose wheat OT - forage yield OT - marker-assisted selection COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:33 PHST- 2026/08/11 00:00 [received] PHST- 2026/09/07 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - cimb48100973 [pii] AID - cimb-48-00973 [pii] AID - 10.3390/cimb48100973 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Sep 22;48(10):973. doi: 10.3390/cimb48100973. PMID- 42939506 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 1467-3045 (Electronic) IS - 1467-3037 (Print) IS - 1467-3037 (Linking) VI - 48 IP - 10 DP - 2026 Sep 22 TI - Targeting PI3Kalpha with Small Molecules: Progress and Challenges in Tumor Treatment. LID - 10.3390/cimb48100968 [doi] LID - 968 AB - Aberrant activation of PI3Kalpha tightly correlates with tumorigenesis and advancement. PI3Kalpha inhibitors can bring significant clinical gains to patients with PIK3CA-mutant breast cancer and other solid tumor patients by inhibiting tumor cell growth and regulating the immune microenvironment. Alpelisib, a selective PI3Kalpha inhibitor, has a more favorable benefit-to-risk ratio compared with earlier-generation pan-PI3K inhibitors, with more manageable risks of severe adverse events, including pneumonitis. CYH33 induces immune activation and synergizes with a FASN inhibitor to further promote antitumor immunity. In addition, inavolisib, which features a mutant-selective degradation mechanism, has already been approved for clinical use, while compounds with distinct mechanisms, such as the wild-type PI3Kalpha-sparing allosteric inhibitor STX-478, have demonstrated favorable preclinical results and are currently under phase I/II clinical research for PIK3CA-mutated advanced solid tumors. Apart from the above therapies, emerging drugs with differentiated mechanisms have shown great potential for clinical application in the years ahead. BEBT-908 not only inhibits the growth of tumor cells but also activates host anti-tumor immunity to strengthen immune checkpoint treatment. Additionally, ZM-PI05 represents a PROTAC agent that degrades the PI3Kalpha protein. Despite the evident clinical advantages of PI3Kalpha inhibitors, toxicities such as hyperglycemia, rash, and gastrointestinal reactions limit their popularization. Current research mainly focuses on designing selective PI3Kalpha inhibitors with improved safety and establishing optimal medication regimens. This paper discusses the biological roles of PI3Kalpha in tumors, summarizes clinical trials, common side effects, and binding characteristics of PI3Kalpha inhibitors, and proposes guidance for their further development and utilization. FAU - Jia, Wenqing AU - Jia W AD - College of Chemistry and Chemical Engineering, Qilu Normal University, Jinan 250200, China. LA - eng GR - 2020KJ186/Tianjin Municipal Education Commission/ PT - Journal Article PT - Review DEP - 20260922 PL - Switzerland TA - Curr Issues Mol Biol JT - Current issues in molecular biology JID - 100931761 PMC - PMC13703482 OTO - NOTNLM OT - PI3Kalpha OT - PI3Kalpha inhibitor OT - clinical trial OT - drug development OT - tumor COIS- The author declares no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:33 PHST- 2026/08/20 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - cimb48100968 [pii] AID - cimb-48-00968 [pii] AID - 10.3390/cimb48100968 [doi] PST - epublish SO - Curr Issues Mol Biol. 2026 Sep 22;48(10):968. doi: 10.3390/cimb48100968. PMID- 42939502 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 21 TI - Grazing Management Regimen Shapes Soil Carbon Stocks and Associated Bacterial Community Composition in a Semi-Arid Rangeland. LID - 10.3390/biology15201901 [doi] LID - 1901 AB - Semi-arid rangelands are highly vulnerable to climate change and overgrazing, yet the functional coupling between soil carbon stabilization and microbial recovery remains largely unquantified. This study was carried out in Northern Mexico (24 degrees 0'33'' N, 104 degrees 42'28'' W, 1990 m.a.s.l.) and evaluated associations between a grazing management gradient comprising continuous grazing (CG), closed non-selective intensive grazing (GE), and open non-selective intensive grazing (SG) on soil carbon stocks and different labile fractions, bacterial community structures, and functional metabolic pathways in semi-arid soils. The results show that SG showed the highest observed organic and inorganic carbon stocks (SOC and SIC stocks, respectively), along with the labile carbon (LC) fraction. Conversely, GE favors the accumulation of stable, non-labile carbon (NLC). Although alpha diversity remained unaffected, grazing management induced differences in beta diversity. Redundancy and LEfSe analyses revealed a strong coupling between carbon fractions and microbial assembly, characterized by the exclusive enrichment of copiotrophic biomarker families (Burkholderiaceae and Sphingobacteriaceae) under SG. Furthermore, predictive functional profiling (PICRUSt2 and ANCOM) showed that pathways such as tRNA charging and nicotinate degradation were enriched under SG and GE, reflecting potential metabolic capacities and inferred microbial adjustments to heterogeneous, excreta-derived nutrient microenvironments during intermediate recovery states. Ultimately, the findings suggest that targeted rotational grazing may contribute to improved soil carbon stocks and microbial community recovery in semi-arid rangelands. FAU - Basave-Villalobos, Erickson AU - Basave-Villalobos E AUID- ORCID: 0000-0002-6743-3623 AD - CIRPAS-Iguala Experiment Station, Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias, Iguala de la Independencia 40000, Guerrero, Mexico. FAU - Sigala, Jose A AU - Sigala JA AD - CIRNOC-Valle del Guadiana Experiment Station, Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias, Durango 34170, Durango, Mexico. FAU - Jacobo-Salcedo, Maria Del Rosario AU - Jacobo-Salcedo MDR AD - National Center of Disciplinary Research in Plant-Water-Soil-Atmosphere Relationship (CENID-RASPA), Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias (INIFAP), Gomez Palacio 35079, Durango, Mexico. FAU - Sanchez-Cohen, Ignacio AU - Sanchez-Cohen I AD - National Center of Disciplinary Research in Plant-Water-Soil-Atmosphere Relationship (CENID-RASPA), Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias (INIFAP), Gomez Palacio 35079, Durango, Mexico. FAU - Constante-Garcia, Vicenta AU - Constante-Garcia V AD - National Center of Disciplinary Research in Plant-Water-Soil-Atmosphere Relationship (CENID-RASPA), Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias (INIFAP), Gomez Palacio 35079, Durango, Mexico. FAU - Sanchez-Ledesma, Judith Abigail AU - Sanchez-Ledesma JA AUID- ORCID: 0000-0003-0989-9086 AD - Tecnologico Nacional de Mexico, Instituto Tecnologico de Torreon, Torreon 27170, Coahuila, Mexico. FAU - Nava-Reyna, Erika AU - Nava-Reyna E AUID- ORCID: 0000-0003-2296-2158 AD - National Center of Disciplinary Research in Plant-Water-Soil-Atmosphere Relationship (CENID-RASPA), Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias (INIFAP), Gomez Palacio 35079, Durango, Mexico. LA - eng GR - 0265435769/Instituto Nacional de Investigaciones Forestales Agricolas y Pecuarias/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701752 OTO - NOTNLM OT - carbon stocks OT - grazing management OT - microbiome OT - rangeland resilience COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:33 PHST- 2026/08/30 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - biology15201901 [pii] AID - biology-15-01901 [pii] AID - 10.3390/biology15201901 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 21;15(20):1901. doi: 10.3390/biology15201901. PMID- 42939495 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 20 TI - Environmental Stress and Feed Intake in Chickens: Neuroendocrine Mechanisms and Mitigation Strategies. LID - 10.3390/biology15201894 [doi] LID - 1894 AB - Environmental stresses negatively affect feed intake in chickens, leading to reduced growth rates and significant economic losses. This review examined the molecular mechanisms of stress-induced loss of appetite, focusing on the hypothalamic regulation of appetite-associated neuropeptides, the role of the HPA axis, elevated CORT levels, and stress-induced inflammatory cytokines that inhibit appetite-stimulating signals. Current mitigation strategies, such as cooling systems, nutritional supplements, early-life programs and genetic selection using QTL mapping, have been shown to provide varying levels of effectiveness. Limited understanding of how multiple stressors act in concert at the cellular level in natural environments is a major limitation. Furthermore, the majority of studies focus on acute stress, while low-grade chronic stress models are underrepresented despite their commercial importance. A significant shortcoming is the absence of validated non-invasive biomarkers to predict stress-induced feed reductions before production losses begin. This review examines the molecular neuroendocrine mechanisms of stress-induced anorexia and the available mitigation strategies, highlighting the disconnect between mechanistic understanding and current interventions, and emphasizing the need for mechanism-based proactive measures to sustainably improve feed intake and productivity in stressed chickens. Therefore, future research should focus on integrated multi-stress frameworks and the evaluation of the lasting epigenetic effects of early-life interventions in chickens. FAU - Adam, Saber Y AU - Adam SY AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. AD - Biomedical Research Institute, Darfur University College, Nyala 63313, Sudan. FAU - Husien, Hosameldeen Mohamed AU - Husien HM AUID- ORCID: 0009-0008-8993-3507 AD - College of Veterinary Medicine, University of Albutana, Rufaa 22217, Sudan. FAU - Mathapo, Madumetja Cyril AU - Mathapo MC AD - Department of Agricultural Economics and Animal Production, University of Limpopo, Private Bag X1106, Sovenga, Limpopo 0727, South Africa. FAU - Ahmed, Abdelkareem A AU - Ahmed AA AD - Biomedical Research Institute, Darfur University College, Nyala 63313, Sudan. AD - Department of Veterinary Sciences, Faculty of Animal and Veterinary Sciences, Botswana University of Agriculture and Natural Resources, Gaborone P.O. Box 100, Botswana. FAU - Essa, Mohamed Osman Abdalrahem AU - Essa MOA AD - College of Veterinary Medicine, University of Albutana, Rufaa 22217, Sudan. FAU - Abdulrahman, Mudathir Y AU - Abdulrahman MY AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. AD - Biomedical Research Institute, Darfur University College, Nyala 63313, Sudan. FAU - Tyasi, Thobela Louis AU - Tyasi TL AUID- ORCID: 0000-0002-3519-7806 AD - Department of Agricultural Economics and Animal Production, University of Limpopo, Private Bag X1106, Sovenga, Limpopo 0727, South Africa. FAU - Cai, Demin AU - Cai D AUID- ORCID: 0000-0003-0500-5292 AD - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. LA - eng PT - Journal Article PT - Review DEP - 20261020 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701440 OTO - NOTNLM OT - climate change OT - corticosterone OT - endocrinology OT - epigenetics OT - microbiome-gut-brain axis OT - poultry welfare COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:33 PHST- 2026/09/13 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/18 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - biology15201894 [pii] AID - biology-15-01894 [pii] AID - 10.3390/biology15201894 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 20;15(20):1894. doi: 10.3390/biology15201894. PMID- 42939486 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 19 TI - Constitutive Expression Differences and Thermal Responses of Stress-Associated Transcripts in Pacific Abalone. LID - 10.3390/biology15201885 [doi] LID - 1885 AB - We examined basal and exposure-associated transcript abundance in Pacific abalone. Pi3k, Akt, mTOR, Hsf1, Hsp70, and Hsp90 were compared across five anatomical samples and between heat-selected and common populations after identical two-week acclimation. Five transcripts were examined during adult exposure to 30 degrees C for up to 72 h and after a 15 min larval heat shock. Basal groups had three animal samples; population and adult groups had two or three analyzed samples. Larval groups comprised three pools of 500 individuals. Qualitative histology used three animals per condition. Welch tests on log2 values used Holm correction. Selected animals had higher hepatopancreatic Pi3k, Akt, mTOR, and Hsp70 levels. Hepatopancreatic Hsf1/Hsp70 means were highest at 12 h and Pi3k/Akt means at 24 h; peak times were not statistically compared. All five transcripts increased within late-veliger samples, without establishing a stage-by-treatment interaction. Histology showed local discontinuities, including in controls. These patterns identify candidate transcript-level indicators. One adult tank per condition, absent time-matched control comparisons, unverified larval culture independence, small samples, normalization uncertainty, population-history confounding, and absent protein endpoints and direct tolerance measurements limit interpretation. FAU - Cui, Xuefan AU - Cui X AD - State Key Laboratory of Mariculture Breeding, Key Laboratory of Marine Biotechnology of Fujian Province, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Lan, Di AU - Lan D AD - State Key Laboratory of Mariculture Breeding, Key Laboratory of Marine Biotechnology of Fujian Province, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Lin, Huaqing AU - Lin H AD - State Key Laboratory of Mariculture Breeding, Key Laboratory of Marine Biotechnology of Fujian Province, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Yang, Weilong AU - Yang W AD - State Key Laboratory of Mariculture Breeding, Key Laboratory of Marine Biotechnology of Fujian Province, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Liu, Xin AU - Liu X AD - State Key Laboratory of Mariculture Breeding, Key Laboratory of Marine Biotechnology of Fujian Province, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Liu, Qiwen AU - Liu Q AD - State Key Laboratory of Mariculture Breeding, Key Laboratory of Marine Biotechnology of Fujian Province, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Nian, Shushan AU - Nian S AD - State Key Laboratory of Mariculture Breeding, Key Laboratory of Marine Biotechnology of Fujian Province, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. FAU - Li, Zhaoxian AU - Li Z AD - Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China. FAU - Chen, Xinhua AU - Chen X AD - State Key Laboratory of Mariculture Breeding, Key Laboratory of Marine Biotechnology of Fujian Province, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. AD - Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China. FAU - Di, Guilan AU - Di G AD - State Key Laboratory of Mariculture Breeding, Key Laboratory of Marine Biotechnology of Fujian Province, College of Marine Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China. LA - eng GR - 2026J002023/Natural Science Foundation of Fujian Province of China/ GR - 2025N0064/Fujian Provincial Science and Technology Special Mission Service Industry Chain Special Project-Agricultural Guiding (Key) Projects/ GR - U23A20253/National Natural Science Foundation of China/ GR - 2021F02/Fuzhou Institute of Oceanography/ GR - MBL26IR08/Independent Research Project of State Key Laboratory of Mariculture Breeding/ GR - 2026KF003/open project of State Key Laboratory of Mariculture Breeding/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701634 OTO - NOTNLM OT - Haliotis discus hannai OT - PI3K-AKT-mTOR OT - constitutive expression OT - heat-shock response OT - hepatopancreas OT - population variation OT - thermal stress COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:33 PHST- 2026/09/22 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - biology15201885 [pii] AID - biology-15-01885 [pii] AID - 10.3390/biology15201885 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 19;15(20):1885. doi: 10.3390/biology15201885. PMID- 42939485 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 19 TI - Host-Response Engineering in Regenerative Cardiology: Mechanistic Boundaries Between Remuscularization and Immune Reprogramming. LID - 10.3390/biology15201884 [doi] LID - 1884 AB - Regenerative cardiology has bifurcated into true remuscularization and host-response engineering. The first uses pluripotent-stem-cell-derived cardiomyocytes or engineered heart muscle to achieve durable contractile replacement; the second comprises adult stromal-cell products, extracellular vesicle preparations and biomaterials that may redirect endogenous repair without generating meaningful myocardium. This mechanistic narrative review reconstructs post-ischemic sterile inflammation as a temporally and spatially organized program and evaluates whether these platforms converge on shared causal pathways. Evidence is classified claim by claim using separate descriptive categories for therapeutic efficacy and mechanistic causal support. The platforms diverge proximally through administered-cell death and clearance, vesicle biodistribution and cargo transfer, or material-tissue interactions. Their most defensible distal convergence is incomplete and centers on monocyte/macrophage balance, efferocytosis, immune-fibroblast signaling and the macrophage-fibroblast-extracellular-matrix axis. Causal support is strong for selected mediators and preparations but cannot be generalized across product classes. No cross-platform convergence on therapeutically meaningful cardiomyocyte generation has been demonstrated. Human studies show limited target-engagement signals without evidence that they localize to myocardium or mediate benefit. Route, timing, disease stage and product identity should therefore be treated as mechanistic variables in preclinical design and clinical translation. FAU - Bonanni, Luca AU - Bonanni L AUID- ORCID: 0009-0004-6360-5653 AD - Department of Medicine, Ospedale dell'Angelo, 30174 Venice, Italy. AD - Department of Medicine, University of Padua, 35122 Padova, Italy. FAU - Ferri, Nicola AU - Ferri N AUID- ORCID: 0000-0001-8898-7441 AD - Department of Medicine, University of Padua, 35122 Padova, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701815 OTO - NOTNLM OT - biomaterials OT - cardiac regeneration OT - efferocytosis OT - extracellular vesicles OT - host-response engineering OT - macrophages OT - mesenchymal stromal cells OT - myocardial infarction OT - remuscularization OT - sterile inflammation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:33 PHST- 2026/08/24 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - biology15201884 [pii] AID - biology-15-01884 [pii] AID - 10.3390/biology15201884 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 19;15(20):1884. doi: 10.3390/biology15201884. PMID- 42939480 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 18 TI - Microbial Rhodopsins: Structural-Functional Mechanisms and the Design of Optogenetic Silencing Tools-With Emphasis on Halorubrum Archaeorhodopsins. LID - 10.3390/biology15201879 [doi] LID - 1879 AB - Microbial rhodopsins are light-sensitive membrane proteins that use retinal as a covalent chromophore and adopt a seven-transmembrane alpha-helical fold. They are widely distributed across archaea, bacteria, algae, and fungi and mediate diverse functions, including light-driven ion transport, light-gated ion conduction, and photosensory signaling. Since the discovery of bacteriorhodopsin in 1971, the microbial rhodopsin family has expanded continuously, and channelrhodopsin-based optogenetics has revolutionized neuroscience by enabling millisecond-scale optical control of neuronal activity. However, compared with excitatory tools, optogenetic silencing tools have developed more slowly and remain limited by insufficient photosensitivity, poor membrane localization, ion-homeostasis perturbation, and phototoxicity. Because these properties are largely constrained by the structural and functional features of the parent microbial proteins, the discovery and in-depth characterization of natural rhodopsins remain essential for tool innovation. This narrative review focuses on ion-pumping archaeorhodopsins, with emphasis on the genus Halorubrum and on HeAR, and describes how the literature was selected. We first summarize the functional classification, structural basis, photocycle, and ion-pumping mechanisms of microbial rhodopsins. We then discuss the diversity of Halorubrum archaeorhodopsins and the structural-functional features of HeAR, a recently characterized archaeorhodopsin from a halophilic archaeon isolated from Ejinoor Salt Lake in Inner Mongolia, China. HeAR absorbs at 550 nm (dark-adapted) and 560 nm (light-adapted), essentially the same maximum as AR3 (559 nm). Its Schiff-base counterion Asp-95 has a pKa of 3.5 +/- 0.1, about one unit higher than BR Asp-85 ( approximately 2.6) and AR3 Asp-95 (2.5 +/- 0.2), but matched by AR4 Asp-85 (3.6); thus, this is a property that HeAR shares with its closest relative rather than one that sets it apart. Its photocycle ( approximately 100 ms) is prolonged-roughly ten times longer than the overall cycle of BR (11 ms), of AR4 ( approximately 10 ms) and of the O-decay of AR3 (tau approximately 14 ms)-while its M intermediate forms and decays faster than in BR; relative to AR3 and AR4, however, the M decay is slower (3.3 ms against approximately 390 micros and 0.55/3 ms) and the overall cycle is longer. These are established in vitro properties of the heterologously expressed protein, and because a pump moves a fixed, small number of charges per photocycle, low-light performance remains a hypothesis to be tested rather than a demonstrated advantage. In this review "red-shifted" is used in one sense only: HeAR's 550-560 nm window lies approximately 80-90 nm to the red of excitatory channelrhodopsins ( approximately 470 nm); relative to BR it is blue-shifted by approximately 18 nm, and relative to AR3 the two absorption maxima coincide. We further review the evolution of optogenetic silencing tools, from inward chloride pumps and outward proton pumps to light-gated anion channels and potassium channels, and discuss structure-guided rational design strategies, including structure prediction, molecular dynamics simulation, directed evolution, and machine learning-assisted engineering. Finally, we outline current challenges and future opportunities in phototoxicity, deep-tissue light delivery, clinical translation and the exploitation of halophilic archaeal resources, and we specify which experiments are required before HeAR can be judged as a silencing tool. Clinically validated optogenetics is kept separate throughout from speculative applications of newly characterized archaeorhodopsins. This review aims to provide a comparative framework linking the structural-functional properties of natural rhodopsins to the rational design of next-generation optogenetic silencing tools. FAU - Chao, Luomeng AU - Chao L AUID- ORCID: 0009-0007-0697-7516 AD - College of Animal Science and Technology, Inner Mongolia Minzu University, Tongliao 028000, China. FAU - Yang, Yuxia AU - Yang Y AUID- ORCID: 0000-0002-1266-9698 AD - College of Computer Science and Technology, Inner Mongolia Minzu University, Tongliao 028000, China. LA - eng GR - 2024ZY0054/Central Government-Guided Local Science and Technology Development Fund Project of Inner Mongolia Autonomous Region/ GR - 2026MS0644/Natural Science Foundation of Inner Mongolia Autonomous Region/ PT - Journal Article PT - Review DEP - 20261018 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701716 OTO - NOTNLM OT - Halorubrum OT - HeAR OT - archaerhodopsin OT - bacteriorhodopsin OT - ion pump OT - microbial rhodopsin OT - optogenetic silencing OT - photocycle OT - protein engineering OT - retinal COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:33 PHST- 2026/09/22 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - biology15201879 [pii] AID - biology-15-01879 [pii] AID - 10.3390/biology15201879 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 18;15(20):1879. doi: 10.3390/biology15201879. PMID- 42939479 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 18 TI - Host-Fungus Interactions Link Autoimmune and Immune-Mediated Inflammatory Diseases to Gastrointestinal Cancer: Lessons from KA/KA and Card9(-/-) Mouse Models. LID - 10.3390/biology15201878 [doi] LID - 1878 AB - Autoimmune diseases, immune-mediated inflammatory diseases, and cancer are distinct pathological conditions that can intersect through immune dysregulation, chronic inflammation, impaired host defense, and altered host-microbe interactions. In selected settings, autoimmune and immune-mediated inflammatory diseases are associated with increased gastrointestinal cancer risk. Although bacterial and viral contributions to inflammation-associated cancer have been extensively studied, the role of fungi remains less investigated. This Opinion article discusses host-fungus interactions through two experimental mouse models that connect altered immune tolerance, innate antifungal responses, and gastrointestinal tumorigenesis. Kinase-dead IKKalpha K44A knock-in mice develop defective central immune tolerance, APECED-like autoimmune manifestations, mucosal fungal infection, epithelial EGFR signaling, and esophageal squamous cell carcinoma. By contrast, Card9(-/-) mice provide a distinct model in which impaired innate antifungal immunity alters intestinal fungal ecology and myeloid-cell responses in colitis-associated colorectal cancer. In the CARD9 setting, fungi may exert pathogenic or protective effects depending on host genotype, immune context, fungal species, and mycobiome structure. Together, these models provide useful tools for investigating how fungi may contribute to gastrointestinal tumorigenesis through cancer-promoting inflammation and immune suppression in a context-dependent manner. However, these findings do not yet validate clinical strategies and should not be interpreted as broadly representative of all autoimmune or immune-mediated inflammatory diseases. Further mechanistic and human studies are needed. FAU - Zhu, Joyce AU - Zhu J AD - Cancer Innovation Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA. FAU - Rawal, Diya AU - Rawal D AD - Cancer Innovation Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA. FAU - Cheng, Adeline AU - Cheng A AUID- ORCID: 0009-0009-0852-7935 AD - Cancer Innovation Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA. FAU - Tross, Debra AU - Tross D AUID- ORCID: 0000-0001-7505-4394 AD - Cancer Innovation Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA. FAU - Li, Xin AU - Li X AD - Cancer Innovation Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA. FAU - Yoshimura, Teizo AU - Yoshimura T AUID- ORCID: 0000-0002-3853-2075 AD - Cancer Innovation Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA. FAU - Chen, Keqiang AU - Chen K AUID- ORCID: 0000-0003-0343-3891 AD - Cancer Innovation Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA. FAU - Hu, Yinling AU - Hu Y AD - Cancer Innovation Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA. FAU - Zhu, Feng AU - Zhu F AUID- ORCID: 0000-0001-9630-273X AD - Cancer Innovation Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702, USA. LA - eng GR - ZIA BC011212/ImNIH/Intramural NIH HHS/United States GR - ZIA BC011391/ImNIH/Intramural NIH HHS/United States PT - Comment PT - Journal Article DEP - 20261018 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701252 OTO - NOTNLM OT - APECED OT - CARD9 OT - IKKalpha OT - autoimmune disease OT - colorectal cancer OT - esophageal squamous cell carcinoma OT - gastrointestinal cancer OT - host-fungus interactions OT - immune-mediated inflammatory disease OT - inflammatory bowel disease COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:33 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - biology15201878 [pii] AID - biology-15-01878 [pii] AID - 10.3390/biology15201878 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 18;15(20):1878. doi: 10.3390/biology15201878. PMID- 42939465 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - Life-Stage and Parasite-Induced Shifts in the Skin Mucus Proteome of Nile tilapia (Oreochromis niloticus). LID - 10.3390/biology15201864 [doi] LID - 1864 AB - Nile tilapia (Oreochromis niloticus) skin mucus serves as a critical primary defense against aquatic pathogens, yet its developmental and infection-induced proteomic dynamics remain poorly characterized. This study utilized liquid chromatography-mass spectrometry (LC-MS)-based comparative proteomics to characterize skin mucus profiles across developmental stages and during monogenean Cichlidarus cichlidarum infection. The analysis identified 7950 proteins, including 1192 core proteins shared by all groups, primarily associated with maintaining physical barrier integrity, responses to oxidative stress, and pathogen recognition. We identified 1328 juvenile-specific proteins enriched in the organophosphate metabolic process, cytoskeletal protein binding and hydrolase activity, complement and coagulation cascades, and phagosome pathways, whereas 833 adult-specific proteins were associated with macromolecule catabolic process, NOD-like (Nucleotide-binding Oligomerization Domain-like) receptor signaling, chemokine signaling, leukocyte transendothelial migration and Toll-like receptor signaling. This may indicate that juvenile tilapia skin mucus is characterized by high metabolic activity and innate immune effectors for broad-spectrum defense against environmental microbes, while adults employ integrated innate-adaptive mechanisms for targeted pathogen clearance. Following C. cichlidarum infection, the markedly increased abundance of 1343 proteins in tilapia skin mucus delineates a highly coordinated defense network. Functional enrichment analysis revealed that these proteins are primarily associated with catalytic activity and are significantly involved in key signaling pathways, including phagosome, C-type lectin receptor signaling, PI3K-Akt signaling, chemokine signaling, and the complement and coagulation cascades. Moreover, 64 C. cichlidarum-derived proteins were identified in the mucus of infected tilapias, including HSC (heat shock cognate protein) 70, serine/threonine protein phosphatase, calmodulin, and thioredoxin peroxidase. These proteins are potentially involved in the host invasion or immune evasion of C. cichlidarum. These results provide insights into the adaptability of fish mucosal immunity and suggest targets for future functional characterization. FAU - Yan, Ning AU - Yan N AD - State Key Laboratory of Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. FAU - Sun, Rui AU - Sun R AD - State Key Laboratory of Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. FAU - Zhuang, Jingyu AU - Zhuang J AD - State Key Laboratory of Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. FAU - Lu, Qianyu AU - Lu Q AD - State Key Laboratory of Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. FAU - Cheng, Jie AU - Cheng J AD - State Key Laboratory of Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. FAU - Xiong, Caijun AU - Xiong C AD - State Key Laboratory of Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. FAU - Liu, Jinyun AU - Liu J AD - State Key Laboratory of Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. FAU - Jiang, Xinyue AU - Jiang X AD - State Key Laboratory of Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. FAU - Huang, Shengfeng AU - Huang S AD - State Key Laboratory of Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. FAU - Chen, Jinsong AU - Chen J AUID- ORCID: 0009-0006-9773-6488 AD - State Key Laboratory of Biocontrol, Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. LA - eng GR - 32373171/National Natural Science Foundation of China/ GR - 2024A1515012750/Guangdong Provincial Academy of Building Research Group/ GR - 2023A04J1420/Guangzhou Institute of Science and Technology/ GR - SML2021SP304/Project of Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai)/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13702020 OTO - NOTNLM OT - Cichlidarus cichlidarum OT - Oreochromis niloticus OT - immunity OT - parasite OT - skin mucus COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:33 PHST- 2026/07/25 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - biology15201864 [pii] AID - biology-15-01864 [pii] AID - 10.3390/biology15201864 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 16;15(20):1864. doi: 10.3390/biology15201864. PMID- 42939463 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - Cold Storage of 1- to 5-Day-Old Mummies and Newly Emerged Adults of Aphelinus maculatus for Biological Control of Myzus persicae. LID - 10.3390/biology15201862 [doi] LID - 1862 AB - Production and field-release schedules are often asynchronous in augmentative biological control. We evaluated the cold-storage tolerance of 1- to 5-day-old mummies and newly emerged adults of Aphelinus maculatus, a parasitoid of Myzus persicae, at 4 and 8 degrees C. Adult emergence from stored mummies and survival of directly stored adults were recorded. Poststorage longevity, host-feeding capacity, parasitism capacity, offspring production, and offspring sex ratio were evaluated for selected cohorts stored at 8 degrees C. Storage at 8 degrees C generally resulted in higher mummy emergence and adult survival than storage at 4 degrees C. Among the tested mummy-age cohorts, 3-day-old mummies showed the highest emergence under the tested conditions. At 8 degrees C, emergence from this cohort was 90.0, 78.3, 64.7, 54.7, and 21.3% after 7, 14, 21, 30, and 60 d, respectively. Directly stored females survived better than males; female survival was 72.0% after 30 d and 20.3% at the 90-day storage endpoint. Increasing storage duration reduced poststorage longevity, host-feeding, parasitism, and offspring production. The female-to-male offspring sex ratio remained relatively stable during the 30 d poststorage assessment period. Under the tested conditions, storing 3-day-old mummies at 8 degrees C provides an option for delayed emergence, whereas direct adult storage at 8 degrees C may be useful for short-term holding. FAU - Wang, Xiaoli AU - Wang X AUID- ORCID: 0009-0006-2467-911X AD - College of Forestry, Inner Mongolia Agricultural University, Hohhot 010019, China. AD - National Field Observation and Research Station (Neimenggu Daxing'anling) for Forest Ecosystem, Genhe 022350, China. FAU - Liu, Qingxian AU - Liu Q AD - Inner Mongolia Autonomous Region Forestry and Grassland Protection Station, Hohhot 010020, China. FAU - Sa, Rula AU - Sa R AD - College of Forestry, Inner Mongolia Agricultural University, Hohhot 010019, China. AD - National Field Observation and Research Station (Neimenggu Daxing'anling) for Forest Ecosystem, Genhe 022350, China. FAU - Zhu, Anqi AU - Zhu A AD - College of Forestry, Inner Mongolia Agricultural University, Hohhot 010019, China. FAU - Dai, Wenhao AU - Dai W AD - Center for Biological Disaster Prevention and Control, National Forestry and Grassland Administration, Shenyang 110034, China. FAU - Yan, Liqiong AU - Yan L AUID- ORCID: 0009-0002-1006-9629 AD - College of Forestry, Inner Mongolia Agricultural University, Hohhot 010019, China. FAU - Zhang, Liyan AU - Zhang L AUID- ORCID: 0000-0002-7215-5138 AD - College of Forestry, Inner Mongolia Agricultural University, Hohhot 010019, China. FAU - Jiang, Haiyan AU - Jiang H AUID- ORCID: 0009-0002-1237-9970 AD - College of Forestry, Inner Mongolia Agricultural University, Hohhot 010019, China. LA - eng GR - BR230106/Inner Mongolia Agricultural University/ GR - 2025LHMS03036/Department of Science and Technology of Inner Mongolia Autonomous Region/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701712 OTO - NOTNLM OT - Aphelinus maculatus OT - Myzus persicae OT - cold storage OT - parasitoid fitness OT - wolfberry COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:33 PHST- 2026/08/04 00:00 [received] PHST- 2026/08/30 00:00 [revised] PHST- 2026/09/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - biology15201862 [pii] AID - biology-15-01862 [pii] AID - 10.3390/biology15201862 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 16;15(20):1862. doi: 10.3390/biology15201862. PMID- 42939461 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 16 TI - Effects of Nepeta cataria and Lactuca sativa on Microbial Community Structure and Nitrogen-Phosphorus Biogeochemical Cycling in Aquaculture Water. LID - 10.3390/biology15201860 [doi] LID - 1860 AB - As the scale of aquaculture continues to expand, aquaculture waters are under increasing environmental pressure, particularly regarding total nitrogen (TN) and total phosphorus (TP). To address this challenge, this study evaluated the efficacy of Nepeta cataria and Lactuca sativa in removing nitrogen and phosphorus from aquaculture water and investigated the underlying microbial mechanisms. The experiment consisted of three groups: the Nepeta cataria group (NC) with Nepeta cataria planted, the Lactuca sativa group (HL) with Lactuca sativa planted, and the control group (CT) without any plants. The results indicated that, compared with the control group, both the Nepeta cataria group and the Lactuca sativa group significantly reduced the concentrations of TAN, nitrite, nitrate, TN, and TP in the aquaculture water (p < 0.01). Plant species exerted a significant regulatory effect on both planktonic and rhizosphere microorganisms. The phyla Proteobacteria, Actinobacteriota, and Bacteroidota were the core dominant phyla across all groups; hgcI_clade, Rhodoluna, and Sediminibacterium were the signature dominant genera in the plant groups' planktonic water. The microbial community is highly coupled with nitrogen and phosphorus nutrients. Nepeta cataria and Lactuca sativa enhanced the nitrification-denitrification coupling process by enriching microbial communities with nitrogen and phosphorus transformation functions, thereby forming a synergistic purification model of "plant absorption-microbial transformation." This study elucidated the regulatory mechanisms by which Nepeta cataria and Lactuca sativa influence microbial communities and nitrogen and phosphorus cycles in aquaculture water, providing a theoretical basis and technical reference for plant selection in aquaponics systems and the ecological purification of aquaculture water. FAU - Wu, Kunjie AU - Wu K AD - School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China. FAU - Hou, Chengliang AU - Hou C AD - School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China. FAU - Zhang, Chen AU - Zhang C AD - School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China. FAU - Ren, Tianyu AU - Ren T AD - School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China. FAU - Meng, Qiang AU - Meng Q AD - School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China. FAU - Yuan, Gaoliang AU - Yuan G AD - School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China. FAU - Ju, Chenxi AU - Ju C AD - School of Fisheries, Xinyang Agriculture and Forestry University, Xinyang 464000, China. LA - eng GR - No.252102111029/People's Government of Henan Province/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701835 OTO - NOTNLM OT - Lactuca sativa OT - Nepeta cataria OT - aquaculture water OT - microbial community structure OT - nitrogen and phosphorus cycles COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:33 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - biology15201860 [pii] AID - biology-15-01860 [pii] AID - 10.3390/biology15201860 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 16;15(20):1860. doi: 10.3390/biology15201860. PMID- 42939455 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Native Entomophages in the Regulation Cameraria ohridella Deschka & Dimic Populations: Potential and Limitations for Control. LID - 10.3390/biology15201854 [doi] LID - 1854 AB - Cameraria ohridella Deschka & Dimic was recorded from Macedonia in the 1980s (Deschka G, Dimic N (1986)). The primary host plant is Aesculus hippocastanum. In Russia, this pest was first discovered in 2003 in Kaliningrad. Since the first introduction of C. ohridella into Europe, many studies have been conducted on C. ohridella parasitoids to identify natural control factors. In this review, we examine the spread of invasive insects identified in recent years, particularly Ohrid horse chestnut leaf miner (Cameraria ohridella Deschka & Dimic), and consider the strategies used by native parasitoids to defeat this phytophage. The review also discusses entomophages that feed on C. ohridella. Studies show that in Europe, C. ohridella is attacked by a diverse range of parasitoids-predominantly native polyphagous Hymenoptera, with the family Eulophidae predominating. The most significant species are M. frontalis, P. saulius, C. trifasciatus, and P. agraules. Significant regional differences in recorded parasitism levels-ranging from 1% to 33.6%-have been identified; these may be attributed not only to biological and ecological factors (such as host developmental stage preferences, as well as spatial, climatic, and phenological conditions) but also to the calculation methodology. The low effectiveness of the C. ohridella parasitoid complex in Europe is largely due to poor developmental synchrony between the parasitoids and their hosts. The composition of the assemblage in urban plantings is also influenced by habitat type and anthropogenic disturbance, particularly the removal of fallen leaves. Finally, we provide an overview of future research prospects, with an emphasis on the influence of parasitoids on C. ohridella population densities. FAU - Bogoutdinova, Liliya R AU - Bogoutdinova LR AUID- ORCID: 0000-0002-3942-4316 AD - N.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Botanicheskaya 4, Moscow 127276, Russia. FAU - Savenko, Ekaterina M AU - Savenko EM AD - N.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Botanicheskaya 4, Moscow 127276, Russia. FAU - Gulevich, Alexander A AU - Gulevich AA AUID- ORCID: 0000-0003-4399-2903 AD - All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, Moscow 127550, Russia. FAU - Baranova, Ekaterina N AU - Baranova EN AUID- ORCID: 0000-0001-8169-9228 AD - N.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, Botanicheskaya 4, Moscow 127276, Russia. AD - All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, Moscow 127550, Russia. LA - eng GR - 25-26-00662/Russian Foundation for Basic Research/ PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701256 OTO - NOTNLM OT - Cameraria ohridella OT - entomophages OT - horse chestnut OT - monitoring OT - parasitoids COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:33 PHST- 2026/08/13 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - biology15201854 [pii] AID - biology-15-01854 [pii] AID - 10.3390/biology15201854 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 15;15(20):1854. doi: 10.3390/biology15201854. PMID- 42939454 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - ARMCX3 Regulates Breast Cancer Progression and Cisplatin Responsiveness Through p53-Related Signaling. LID - 10.3390/biology15201853 [doi] LID - 1853 AB - Despite substantial advances in diagnosis and treatment, breast malignancy continues to cause considerable morbidity and mortality. Cisplatin has demonstrated therapeutic activity in selected patients with advanced breast cancer, but its clinical use may be limited by treatment resistance. Although ARMCX3 is implicated in other cancers, its role and underlying mechanisms in breast cancer remain unclear. We investigated ARMCX3 functions using depletion and rescue experiments, and ARMCX3 depletion reduced cell growth, migration, and invasion and increased cisplatin sensitivity in vitro, while re-expression rescued these phenotypes. Transcriptomic analysis revealed enrichment of the p53 pathway following ARMCX3 depletion. PFT-alpha treatment suppressed the protein level of p53 and attenuated the effects of ARMCX3 knockdown on cell proliferation, migration, invasion, cell-cycle arrest, and cisplatin response. ARMCX3 knockdown was also associated with altered DNA damage response signaling, enhanced cisplatin-induced apoptosis and suppressed tumor growth in vivo, and all these effects were attenuated by PFT-alpha treatment. These findings suggest that p53-associated signaling contributes to the effects of ARMCX3 depletion on breast cancer progression and cisplatin responsiveness. These results may help inform future studies exploring ARMCX3-associated signaling in the context of cisplatin response. FAU - Deng, Xinyan AU - Deng X AD - Sichuan Provincial Engineering Laboratory for Prevention and Control Technology of Veterinary Drug Residue in Animal-Origin Food, School of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China. AD - Experiment Teaching Demonstration Center of Laboratory Medicine, School of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China. FAU - Ma, Haixiang AU - Ma H AD - Sichuan Provincial Engineering Laboratory for Prevention and Control Technology of Veterinary Drug Residue in Animal-Origin Food, School of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China. AD - Experiment Teaching Demonstration Center of Laboratory Medicine, School of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China. FAU - Zhang, Jiajie AU - Zhang J AD - Sichuan Provincial Engineering Laboratory for Prevention and Control Technology of Veterinary Drug Residue in Animal-Origin Food, School of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China. AD - Experiment Teaching Demonstration Center of Laboratory Medicine, School of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China. FAU - Huang, Xiyang AU - Huang X AD - Sichuan Provincial Engineering Laboratory for Prevention and Control Technology of Veterinary Drug Residue in Animal-Origin Food, School of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China. AD - Experiment Teaching Demonstration Center of Laboratory Medicine, School of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China. FAU - Cai, Minghong AU - Cai M AD - School of Bioscience and Technology, Chengdu Medical College, Chengdu 610500, China. FAU - Ge, Fanghui AU - Ge F AD - Sichuan Provincial Engineering Laboratory for Prevention and Control Technology of Veterinary Drug Residue in Animal-Origin Food, School of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China. AD - Experiment Teaching Demonstration Center of Laboratory Medicine, School of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China. FAU - Zhang, Hong AU - Zhang H AD - Sichuan Provincial Engineering Laboratory for Prevention and Control Technology of Veterinary Drug Residue in Animal-Origin Food, School of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China. AD - Experiment Teaching Demonstration Center of Laboratory Medicine, School of Laboratory Medicine, Chengdu Medical College, Chengdu 610500, China. FAU - Wang, Jiandong AU - Wang J AUID- ORCID: 0000-0002-4694-423X AD - School of Bioscience and Technology, Chengdu Medical College, Chengdu 610500, China. LA - eng GR - CYZZD24-09/Chengdu Medical College/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701251 OTO - NOTNLM OT - ARMCX3 OT - breast cancer OT - cisplatin sensitivity OT - p53 signaling COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:33 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - biology15201853 [pii] AID - biology-15-01853 [pii] AID - 10.3390/biology15201853 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 15;15(20):1853. doi: 10.3390/biology15201853. PMID- 42939449 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 15 TI - Genetic Improvement Strategies for Enhancing Feed Efficiency in Aquaculture. LID - 10.3390/biology15201849 [doi] LID - 1849 AB - Feed efficiency (FE) is one of the most economically and environmentally important target traits for genetic improvement since feed accounts for 50-70% of total production expenses in aquaculture worldwide. In addition to lowering production costs, reducing feed consumption per unit of biomass output also reduces emissions of nitrogen and phosphorus, which is in line with global sustainability goals for food production. The genetic framework of FE traits, molecular dissection of the targeted loci, conventional selective breeding frameworks, genomic selection, emerging biotechnological applications, major implementation challenges, and prospects are all covered in this review, which summarizes current developments in genetic improvement of FE in aquaculture. We incorporate empirical data from important farmed finfish and crustacean species, emphasizing that FE-related traits show measurable genetic variation available for selection, although reported heritability estimates vary substantially among traits, populations, phenotyping methods, and production conditions. Compared to conventional breeding techniques, the integration of high-throughput phenotyping, genomic prediction and multi-omics approaches has expanded opportunities to characterize FE variation and improve selection accuracy. However, most reported candidate loci and genes remain supported by associative rather than causal evidence, and functional validation in aquaculture remains limited. Integrated breeding approaches therefore offer feasible pathways to mitigate major constraints to genetic improvement of FE, although their practical application differs substantially among species and production systems. FAU - Abdullahi, Usman AU - Abdullahi U AUID- ORCID: 0009-0002-8493-2077 AD - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China. FAU - Kandeh, Ibrahim AU - Kandeh I AD - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China. FAU - Li, Jianlin AU - Li J AD - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China. AD - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. FAU - Xu, Yuanfeng AU - Xu Y AD - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China. AD - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. FAU - Tang, Yongkai AU - Tang Y AD - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China. AD - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. FAU - Zhao, Yongfeng AU - Zhao Y AD - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China. AD - Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China. LA - eng GR - 2023TD40/China Agriculture Research System/ PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701401 OTO - NOTNLM OT - aquaculture OT - feed efficiency OT - gene editing OT - genetic improvement OT - selective breeding COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:33 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - biology15201849 [pii] AID - biology-15-01849 [pii] AID - 10.3390/biology15201849 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 15;15(20):1849. doi: 10.3390/biology15201849. PMID- 42939446 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Coordination and Variation of Photosynthetic Pigments Across Seedling Ages and Grades Reveal Adaptive Strategies in Pinus yunnanensis. LID - 10.3390/biology15201844 [doi] LID - 1844 AB - Pinus yunnanensis is a vital native species for afforestation in Southwest China, and seedling quality determines restoration outcomes. Seedling grading is widely applied to select robust seedlings, whereas physiological distinctions among seedlings of different grades remain unclear. In the present study, allometric scaling, coefficient-of-variation calculation and correlation analysis are adopted to investigate pigment traits and resource-allocation characteristics of Grade I, Grade II and Grade III seedlings across successive developmental stages. Seedling grade and seedling age exert significant influences on photosynthetic pigment traits. Grade I seedlings exhibit stable pigment composition, steady allometric growth and strong phenotypic stability, with key physiological transitions taking place at 330 d and 510 d. Chlorophyll b showed the highest trait variability. The present results verify the superior adaptability of Grade I seedlings and provide theoretical support for early seedling-quality evaluation and precise seedling cultivation. FAU - Jiang, Ye AU - Jiang Y AD - The Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650233, China. AD - Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Kunming 650224, China. FAU - Lu, Zhuangyue AU - Lu Z AUID- ORCID: 0000-0002-9150-951X AD - The Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650233, China. AD - Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Kunming 650224, China. FAU - Zhang, Qingxue AU - Zhang Q AD - The Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650233, China. AD - Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Kunming 650224, China. FAU - Xue, Tianyi AU - Xue T AD - The Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650233, China. AD - Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Kunming 650224, China. FAU - Kang, Xiangyang AU - Kang X AD - State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. FAU - Xu, Yulan AU - Xu Y AD - The Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650233, China. AD - Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Kunming 650224, China. FAU - Cai, Nianhui AU - Cai N AD - The Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650233, China. AD - Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Kunming 650224, China. FAU - Chen, Lin AU - Chen L AD - The Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Forestry University, Kunming 650233, China. AD - Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Kunming 650224, China. LA - eng GR - 202401BD070001-023/Yunnan Fundamental Research Projects/ GR - 202605AF350003/Yunnan Province Science and Technology Department/ GR - LXXK-2025D01/the Yunnan Provincial First-Class Discipline Construction Fund for Forestry at Southwest Forestry University/ GR - 2024-61/Yunnan Graduate Tutor Team Building Project/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701417 OTO - NOTNLM OT - allometric growth OT - coefficient of variation OT - photosynthetic pigments OT - seedling grading OT - seedling-quality evaluation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:33 PHST- 2026/08/15 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - biology15201844 [pii] AID - biology-15-01844 [pii] AID - 10.3390/biology15201844 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 14;15(20):1844. doi: 10.3390/biology15201844. PMID- 42939441 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - LC-PUFA Biosynthesis in Aquatic Animals: A Hybrid Bibliometric and Thematic Review. LID - 10.3390/biology15201840 [doi] LID - 1840 AB - Long-chain polyunsaturated fatty acids (LC-PUFAs) are essential to the nutrition, metabolism, and physiological performance of aquatic animals. This hybrid review combines bibliometric analysis with focused thematic synthesis to examine three decades (1991-2025) of research on LC-PUFA biosynthesis in aquatic species. Bibliometric trends reveal rising publication output and an expanding research focus on biosynthetic pathways, regulatory mechanisms, and molecular control of LC-PUFA production, with emerging emphasis on metabolomics, environmental regulation, microalgae-derived omega-3 sources, and gene-editing technologies. Thematic analysis reveals that LC-PUFA dynamics are governed by interactions among primary production, trophic transfer, endogenous biosynthetic capacity, and environmental conditions. Marine primary producers serve as major sources of EPA and DHA, whereas freshwater and terrestrial systems often exhibit greater ALA availability and higher n-6/n-3 ratios, influencing LC-PUFA transfer efficiency and consumer adaptation. The review further highlights that aquatic animals have evolved diverse endogenous biosynthetic strategies, including lineage-specific modifications of desaturase and elongase pathways, enabling adaptation to contrasting nutritional environments. LC-PUFA transport across aquatic-terrestrial boundaries through organisms such as emergent insects and amphibians represents an important pathway for transferring essential fatty acids to terrestrial food webs. Furthermore, variation in n-3 LC-PUFA availability contributes to habitat selection and evolutionary diversification, with freshwater environments promoting enhanced biosynthetic capacity through mechanisms such as Fads2 gene duplication. Future directions include structural characterization of biosynthetic enzymes via cryo-electron microscopy and AI-assisted modeling, multi-omics and genome-editing strategies for genetic improvement, and sustainable production approaches including microalgae- and insect-based feeds and integrated multitrophic aquaculture. FAU - Gohar, Faryal AU - Gohar F AD - Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China. AD - International Joint Research Center for the Development and Utilization of Important Mariculture Varieties Surrounding the South China Sea Region, Shantou University, Shantou 515063, China. AD - STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China. FAU - Chao, Bihui AU - Chao B AD - Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China. AD - International Joint Research Center for the Development and Utilization of Important Mariculture Varieties Surrounding the South China Sea Region, Shantou University, Shantou 515063, China. AD - STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China. FAU - Xue, Yongcheng AU - Xue Y AD - Agro-Tech Extension Center of Guangdong Province, Guangzhou 511499, China. FAU - Yuan, Ye AU - Yuan Y AUID- ORCID: 0000-0002-8576-9016 AD - Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China. AD - International Joint Research Center for the Development and Utilization of Important Mariculture Varieties Surrounding the South China Sea Region, Shantou University, Shantou 515063, China. AD - STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China. FAU - Ikhwanuddin, Mhd AU - Ikhwanuddin M AUID- ORCID: 0000-0002-6430-0876 AD - Higher Institute Centre of Excellence (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus 21030, Malaysia. FAU - Ma, Hongyu AU - Ma H AUID- ORCID: 0000-0003-2299-503X AD - Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China. AD - International Joint Research Center for the Development and Utilization of Important Mariculture Varieties Surrounding the South China Sea Region, Shantou University, Shantou 515063, China. AD - STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou 515063, China. LA - eng GR - 2025-MRB-00-001/the 2025 Research on Breeding Technology of Candidate Species for Guangdong Modern Marine Ranching/ GR - 32473150/National Natural Science Foundation of China/ GR - 2026A0202020022, 2023A0505090010/Science and Technology Project of Guangdong Province/ GR - STKJ2024003/Bai/Qian/Wan Project County Innovation Base (Nan'ao County, Shantou) of Guangdong Province/ PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701199 OTO - NOTNLM OT - LC-PUFA biosynthesis OT - aquaculture OT - aquatic animals OT - bibliometric analysis OT - trophic transfer COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:33 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - biology15201840 [pii] AID - biology-15-01840 [pii] AID - 10.3390/biology15201840 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 14;15(20):1840. doi: 10.3390/biology15201840. PMID- 42939438 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Integrated Phenotypic and Transcriptomic Analysis of the Effects of a Novel Arylaminoalcohol on Plasmodium falciparum. LID - 10.3390/biology15201837 [doi] LID - 1837 AB - Malaria remains the leading parasitic infection worldwide, and the effectiveness of antimalarial drugs is increasingly threatened by the emergence of multidrug-resistant Plasmodium falciparum (P. falciparum) strains. In this context, our team previously developed a series of novel aminopyridinemethanol derivatives, among which GBPS336 showed potent nanomolar activity against P. falciparum strains with reduced susceptibility to reference antimalarials. Here, we investigated the effects of GBPS336 on the intraerythrocytic developmental cycle and transcriptional response of the parasite by combining microscopic phenotyping with whole-transcriptome RNA sequencing (Illumina) of synchronized P. falciparum 3D7 cultures exposed or not to the compound. In GBPS336-treated cultures, microscopic analysis revealed reduced parasitaemia, a marked delay and disruption of intraerythrocytic development and morphological alterations indicative of a parasite stress response. Transcriptomic profiling identified 356 differentially expressed genes at H24 post-exposition in ring-stage cultures with enrichment in functional categories associated with erythrocyte invasion and the parasite-host interface. Altogether, these findings demonstrate the antimalarial activity of GBPS336 and highlight invasion-related pathways as a potentially important component of its mechanism of action. Further studies will be required to determine whether these pathways are directly affected and to identify the molecular target(s) underlying the activity of the compound. FAU - Pronnier Lefebvre, Yael AU - Pronnier Lefebvre Y AUID- ORCID: 0009-0001-0972-0747 AD - Service de Parasitologie et Mycologie Medicales, Centre de Biologie Humaine, CHU Amiens-Picardie,1 Rond-Point du Pr Cabrol, 80054 Amiens, France. FAU - Ben Gayed, Ibtissem AU - Ben Gayed I AD - Service de Parasitologie et Mycologie Medicales, Centre de Biologie Humaine, CHU Amiens-Picardie,1 Rond-Point du Pr Cabrol, 80054 Amiens, France. FAU - Aubry, Aurelien AU - Aubry A AD - Agents Infectieux, Resistance et Chimiotherapie (AGIR), UR 4294, Universite de Picardie Jules Verne, Chemin du Thil, 80025 Amiens, France. AD - Service de Virologie, Centre de Biologie Humaine, CHU Amiens-Picardie,1 Rond-Point du Pr Cabrol, 80054 Amiens, France. FAU - Agnamey, Patrice AU - Agnamey P AD - Agents Infectieux, Resistance et Chimiotherapie (AGIR), UR 4294, Universite de Picardie Jules Verne, Chemin du Thil, 80025 Amiens, France. FAU - Mustiere, Romain AU - Mustiere R AD - Agents Infectieux, Resistance et Chimiotherapie (AGIR), UR 4294, Universite de Picardie Jules Verne, Chemin du Thil, 80025 Amiens, France. FAU - Dassonville-Klimpt, Alexandra AU - Dassonville-Klimpt A AUID- ORCID: 0000-0002-2891-9102 AD - Agents Infectieux, Resistance et Chimiotherapie (AGIR), UR 4294, Universite de Picardie Jules Verne, Chemin du Thil, 80025 Amiens, France. FAU - Sonnet, Pascal AU - Sonnet P AUID- ORCID: 0000-0003-0118-9151 AD - Agents Infectieux, Resistance et Chimiotherapie (AGIR), UR 4294, Universite de Picardie Jules Verne, Chemin du Thil, 80025 Amiens, France. FAU - Le Govic, Yohann AU - Le Govic Y AUID- ORCID: 0000-0001-7686-4612 AD - Service de Parasitologie et Mycologie Medicales, Centre de Biologie Humaine, CHU Amiens-Picardie,1 Rond-Point du Pr Cabrol, 80054 Amiens, France. AD - Agents Infectieux, Resistance et Chimiotherapie (AGIR), UR 4294, Universite de Picardie Jules Verne, Chemin du Thil, 80025 Amiens, France. FAU - Totet, Anne AU - Totet A AUID- ORCID: 0000-0002-9784-575X AD - Service de Parasitologie et Mycologie Medicales, Centre de Biologie Humaine, CHU Amiens-Picardie,1 Rond-Point du Pr Cabrol, 80054 Amiens, France. AD - Agents Infectieux, Resistance et Chimiotherapie (AGIR), UR 4294, Universite de Picardie Jules Verne, Chemin du Thil, 80025 Amiens, France. FAU - Damiani, Celine AU - Damiani C AUID- ORCID: 0000-0003-3532-6775 AD - Service de Parasitologie et Mycologie Medicales, Centre de Biologie Humaine, CHU Amiens-Picardie,1 Rond-Point du Pr Cabrol, 80054 Amiens, France. AD - Agents Infectieux, Resistance et Chimiotherapie (AGIR), UR 4294, Universite de Picardie Jules Verne, Chemin du Thil, 80025 Amiens, France. LA - eng GR - Universite de Picardie Jules Verne/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701406 OTO - NOTNLM OT - Plasmodium falciparum OT - RNA-Seq OT - antimalarial compounds OT - arylaminoalcohols OT - gene ontology OT - mechanism of action OT - microscopy OT - transcriptomics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:33 PHST- 2026/09/20 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - biology15201837 [pii] AID - biology-15-01837 [pii] AID - 10.3390/biology15201837 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 14;15(20):1837. doi: 10.3390/biology15201837. PMID- 42939437 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 14 TI - Keystone K-Strategists Shape Microbial Co-Occurrence Networks in Shale-Gas Wellpad Soils in the Field, Sichuan Basin, China. LID - 10.3390/biology15201836 [doi] LID - 1836 AB - Shale-gas extraction imposes persistent, low-intensity, multi-factor disturbances on terrestrial soil ecosystems, driven by flowback-fluid-derived salinization, drilling-residue deposition, and moderate petroleum hydrocarbon contamination. However, microbial community assembly mechanisms and the corresponding ecological strategies under such combined chronic perturbations remain insufficiently understood. This study employed 16S rRNA gene sequencing, quantitative PCR, and molecular ecological network (MEN) analysis to characterize the soil microbiome within the Changning shale-gas field, Sichuan Basin, China. The results revealed that microbial communities in shale-gas wellpad soils formed highly modular (M = 0.786), non-random "small-world" co-occurrence networks, indicating intrinsically stable microbial systems. Via Z(i)-P(i) topological classification, six low-abundance (<0.63%) keystone taxa were identified: three module hubs (OTU11572, OTU14908, OTU11976) and three connectors (OTU8845, OTU13035, OTU8962), phylogenetically affiliated with Acidobacteriota, Chloroflexi, and Actinobacteriota. These keystone organisms exhibited typical K-strategy traits, including high network connectivity (degree = 21-24), metabolic stability, and dependence on inter-taxa cooperative interactions. Significant positive correlations were detected between these K-strategy keystone species and anaerobic functional genes (nirS, nirK, dsrAB) responsible for denitrification and sulfate reduction, highlighting their adaptation to fluctuating redox environments generated by drilling-cutting accumulation. Distance-based redundancy analysis (Db-RDA) further demonstrated that electrical conductivity (representing salinization stress from flowback-water intrusion) and soil organic matter acted as the dominant environmental filters shaping microbial community composition, outweighing the influence of labile inorganic nutrients. Overall, this work provides novel evidence that K-selection dominates microbial community assembly under chronic soil disturbance associated with shale-gas extraction. Keystone K-strategists sustain modular network stability through the combination of metabolic plasticity and anaerobic respiratory adaptation. These findings expand r/K-selection theory to anthropogenically disturbed soil habitats and offer theoretical guidance for soil ecological restoration and risk assessment for shale-gas exploiting regions. FAU - Xu, Jie AU - Xu J AD - Safety and Environmental Protection Supervision and Testing Center, PetroChina Southwest Oil & Gasfield Company, No. 12, Tianfu North Ave., Wuhou District, Chengdu 610051, China. AD - Sichuan Key Laboratory of Shale Gas Geology Evaluation and Efficient Exploitation, National R&D Center of High-Sulfur Gas Reservoirs, No. 218, Tianyan Rd, Shuangliu District, Chengdu 610213, China. FAU - Liu, Yi AU - Liu Y AD - Safety and Environmental Protection Supervision and Testing Center, PetroChina Southwest Oil & Gasfield Company, No. 12, Tianfu North Ave., Wuhou District, Chengdu 610051, China. FAU - Yun, Jian AU - Yun J AD - Research Institute of Safety and Environmental Protection Technology, PetroChina, No. 1, Huanghe North St., Changping District, Beijing 102206, China. FAU - Cheng, Ximing AU - Cheng X AD - Faculty of Education, Monash University, Melbourne, VIC 3800, Australia. FAU - Wang, Erxiao AU - Wang E AD - Safety and Environmental Protection Supervision and Testing Center, PetroChina Southwest Oil & Gasfield Company, No. 12, Tianfu North Ave., Wuhou District, Chengdu 610051, China. FAU - Tang, Chunling AU - Tang C AD - Safety and Environmental Protection Supervision and Testing Center, PetroChina Southwest Oil & Gasfield Company, No. 12, Tianfu North Ave., Wuhou District, Chengdu 610051, China. LA - eng GR - 2024D107-01-06/PetroChina Southwest Oil and Gas Field Company (China)/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701853 OTO - NOTNLM OT - 16S rRNA gene sequencing OT - K-strategy OT - chronic disturbance OT - keystone species OT - molecular ecological network OT - shale-gas wellpad soil COIS- Jie Xu, Yi Liu, Erxiao Wang, and Chunling Tang were employed by the PetroChina Southwest Oil and Gas Field Company (China). Jian Yun was employed by the Research Institute of Safety and Environmental Protection Technology, PetroChina. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The authors declare that this study received funding from PetroChina Southwest Oil and Gasfield Company, China. The funder had no involvement in the study design, data collection, data analysis, data interpretation, the writing of this manuscript, or the decision to submit this work for publication. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:33 PHST- 2026/07/23 00:00 [received] PHST- 2026/09/10 00:00 [revised] PHST- 2026/09/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - biology15201836 [pii] AID - biology-15-01836 [pii] AID - 10.3390/biology15201836 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 14;15(20):1836. doi: 10.3390/biology15201836. PMID- 42939436 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Systematic Screening of Reference Genes for qPCR in Procambarus clarkii: RPL13a Shows Stable Expression Under Non-Ionic Ammonia Stress and Multiple Experimental Conditions. LID - 10.3390/biology15201835 [doi] LID - 1835 AB - Real-time quantitative PCR (qPCR) is a key technique for elucidating the mechanisms underlying environmental stress responses in aquatic animals, and its accuracy relies heavily on stable reference genes for data normalization. In this study, histopathological examination revealed alterations in the gill, hepatopancreas, and muscle tissues of Procambarus clarkii following NH(3) stress. Based on transcriptomic screening and reference genes reported in the literature, 13 candidate reference genes were selected. Their expression levels in 8 different tissues before and after NH(3) stress were detected using qPCR, and their stability was systematically evaluated using multiple algorithms, including geNorm, NormFinder, BestKeeper, Delta CT and RefFinder. The results identified 60S ribosomal protein L13a (RPL13a), ribosomal protein S18 (RPS18) and eukaryotic translation initiation factor 4A (EIF4A) as the three most stable candidates under NH(3) stress, whereas the traditionally used reference genes beta-actin (beta-actin) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) showed relatively low stability under these conditions. geNorm pairwise variation analysis indicated that the inclusion of a third reference gene could further improve normalization. Validation using four NH(3)-responsive genes showed that (RPL13a + RPS18) and (RPL13a + RPS18 + EIF4A) yielded similar log(2) fold-change estimates, supporting their use as normalization options under the tested conditions. It is worth noting that RPL13a remains highly stable across four conditions: different embryonic developmental stages, different ovarian developmental stages, tissues from both males and females, and following infection with white spot syndrome virus (WSSV). These findings contribute to the selecting and validating reference genes for qPCR analyses in Procambarus clarkii under the specific experimental conditions evaluated in this study. FAU - Yuan, Xiudan AU - Yuan X AD - Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410153, China. AD - Yuelushan Laboratory, Changsha 410128, China. FAU - Tian, Xing AU - Tian X AD - Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410153, China. AD - Yuelushan Laboratory, Changsha 410128, China. FAU - Liu, Yuanyuan AU - Liu Y AD - College of Life Sciences, Hunan Normal University, Changsha 410081, China. FAU - He, Zhigang AU - He Z AD - Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410153, China. AD - Yuelushan Laboratory, Changsha 410128, China. FAU - Li, Jinlong AU - Li J AD - Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410153, China. AD - Yuelushan Laboratory, Changsha 410128, China. FAU - Fan, Yunpeng AU - Fan Y AUID- ORCID: 0000-0003-2374-3627 AD - Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China. FAU - Guo, Jiarong AU - Guo J AD - Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410153, China. FAU - He, Yong AU - He Y AD - Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410153, China. AD - Yuelushan Laboratory, Changsha 410128, China. FAU - Deng, Shiming AU - Deng S AD - Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410153, China. AD - Yuelushan Laboratory, Changsha 410128, China. FAU - Wang, Dongwu AU - Wang D AD - Hunan Fisheries Research Institute and Aquatic Products Seed Stock Station, Changsha 410153, China. AD - Yuelushan Laboratory, Changsha 410128, China. LA - eng GR - 2025RC2095/Yuelushan Laboratory Talent Program/ GR - YLS-2025-ZY02044/Yuelushan Laboratory Breeding Program/ GR - CARS-47-39/Earmarked Fund for China Agriculture Research System/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701790 OTO - NOTNLM OT - Procambarus clarkii OT - RPL13a OT - non-ionic ammonia OT - real-time fluorescence quantitative PCR OT - reference gene COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:33 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - biology15201835 [pii] AID - biology-15-01835 [pii] AID - 10.3390/biology15201835 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 13;15(20):1835. doi: 10.3390/biology15201835. PMID- 42939435 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Programmed Frozen Embryo Transfer in the Absence of Corpus Luteum: Impact on Maternal Cardiovascular Adaptation, Endothelial Function and Risk of Preeclampsia. LID - 10.3390/biology15201834 [doi] LID - 1834 AB - Programmed frozen embryo transfer (FET) cycles are an integral part of assisted reproductive technologies (ARTs). They rely exclusively on the administration of exogenous estrogens and progesterone to prepare the endometrium, and they are characterized by the absence of a functional corpus luteum (CL). Pregnancies resulting from FET cycles have been associated with an increased risk of complications related to placental dysfunction, such as preeclampsia and fetal growth restriction (FGR). Besides progesterone production, the CL secretes vasoactive and angiogenic factors essential for the regulatory mechanisms that occur during the early stages of pregnancy. Its absence results in a different endocrine environment, which may be associated with differences in normal maternal cardiovascular remodeling. In pregnant women without CL function, reduced vasodilation, increased systemic vascular resistance, altered cardiac output, and inadequate renal adaptation have been reported. In addition, disturbances in angiogenic balance and trophoblast invasion may contribute to abnormal placentation and the development of preeclampsia. Accordingly, the absence of the CL in programmed FET cycles may represent a critical endocrine disorder, predisposing patients to maternal cardiovascular disturbance and adverse obstetric outcomes. Recognition of this potential endocrine and vascular interaction has important implications for cycle selection, risk stratification, and preventive strategies in the implementation of assisted reproduction protocols. FAU - Gerede, Angeliki AU - Gerede A AUID- ORCID: 0000-0001-9296-3688 AD - Department of Obstetrics and Gynecology, Democritus University of Thrace, 69100 Alexandroupolis, Greece. FAU - Gkaitatzi, Foteini AU - Gkaitatzi F AD - Department of Obstetrics and Gynecology, Democritus University of Thrace, 69100 Alexandroupolis, Greece. FAU - Oikonomou, Efthymios AU - Oikonomou E AUID- ORCID: 0009-0005-2177-953X AD - Department of Obstetrics and Gynecology, Democritus University of Thrace, 69100 Alexandroupolis, Greece. FAU - Papasozomenou, Panayiota AU - Papasozomenou P AUID- ORCID: 0000-0003-2153-2818 AD - School of Health Science, International Hellenic University, 57400 Thessaloniki, Greece. FAU - Kourti, Vasiliki AU - Kourti V AD - Department of Obstetrics and Gynecology, Democritus University of Thrace, 69100 Alexandroupolis, Greece. FAU - Zagorianakou, Nektaria AU - Zagorianakou N AUID- ORCID: 0009-0001-3036-7212 AD - Department of Nursing, School of Health Sciences, University of Ioannina, 45500 Ioannina, Greece. FAU - Tsikouras, Panagiotis AU - Tsikouras P AD - Department of Obstetrics and Gynecology, Democritus University of Thrace, 69100 Alexandroupolis, Greece. FAU - Koutlaki, Nikoletta AU - Koutlaki N AD - Department of Obstetrics and Gynecology, Democritus University of Thrace, 69100 Alexandroupolis, Greece. FAU - Zafrakas, Menelaos AU - Zafrakas M AUID- ORCID: 0000-0001-9357-1950 AD - School of Health Science, International Hellenic University, 57400 Thessaloniki, Greece. FAU - Domali, Ekaterini AU - Domali E AUID- ORCID: 0000-0001-8899-3040 AD - First Department of Obstetrics and Gynecology, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 11528 Athens, Greece. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701501 OTO - NOTNLM OT - corpus luteum OT - hypertensive disorders of pregnancy OT - maternal cardiovascular adaptation OT - preeclampsia OT - programmed frozen embryo transfer COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:33 PHST- 2026/08/30 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - biology15201834 [pii] AID - biology-15-01834 [pii] AID - 10.3390/biology15201834 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 13;15(20):1834. doi: 10.3390/biology15201834. PMID- 42939430 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Living in the Leaf Litter: Integrative Taxonomy of the Trichomycterus nigroauratus Group, a Catfish Clade from an Endangered Tropical Biodiversity Hotspot Supporting Areas of Endemism for Conservation and Two New Species. LID - 10.3390/biology15201829 [doi] LID - 1829 AB - Mountain ranges of southeastern Brazil shelter a great diversity of endemic species, making the local Atlantic Forest among the most important and endangered biodiversity hotspots in the world, but some fish groups are still little known. The primary focus of the current study is the Trichomycterus nigroauratus group, a catfish clade with species living associated with leaf litter, endemic to the upper and middle Rio Paraiba do Sul basin, with all known species described after 2008. We performed an integrative taxonomic approach combining molecular species delimitation analyses and morphology, which inferred a total of six species, two of which are new and here described, in six disjunct areas. Five areas are coincident with distribution areas of other 11 congeners, supporting areas of endemism. We assessed the conservation status by calculating the extent of occurrence and area of occupancy for each of the 16 species occurring in the five areas of endemism. The Serra da Mantiqueira was considered a biodiversity hotspot for trichomycterine catfishes. The approach followed in this study, combining Integrative Taxonomy to delimit areas of endemism with criteria for assessing conservation status, holds great potential as a method for selecting priority areas for biodiversity conservation. FAU - Costa, Wilson J E M AU - Costa WJEM AUID- ORCID: 0000-0002-0428-638X AD - Laboratory of Systematics and Evolution of Teleost Fishes, Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro CEP 21941-902, Brazil. FAU - Barbosa, Maria Anais AU - Barbosa MA AD - Laboratory of Systematics and Evolution of Teleost Fishes, Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro CEP 21941-902, Brazil. FAU - Mattos, Jose Leonardo O AU - Mattos JLO AUID- ORCID: 0000-0002-2890-4955 AD - Laboratory of Systematics and Evolution of Teleost Fishes, Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro CEP 21941-902, Brazil. FAU - Vilardo, Paulo J AU - Vilardo PJ AUID- ORCID: 0000-0002-0033-9421 AD - Laboratory of Systematics and Evolution of Teleost Fishes, Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro CEP 21941-902, Brazil. FAU - Amorim, Pedro F AU - Amorim PF AUID- ORCID: 0000-0001-7029-0275 AD - Laboratory of Systematics and Evolution of Teleost Fishes, Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro CEP 21941-902, Brazil. FAU - Petrungaro, Marina AU - Petrungaro M AUID- ORCID: 0009-0001-7390-0961 AD - Laboratory of Systematics and Evolution of Teleost Fishes, Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro CEP 21941-902, Brazil. FAU - Katz, Axel M AU - Katz AM AUID- ORCID: 0000-0002-2933-7163 AD - Laboratory of Systematics and Evolution of Teleost Fishes, Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro CEP 21941-902, Brazil. LA - eng GR - 301830/2026-6, 403664/2025-0/National Council for Scientific and Technological Development/ GR - E-26/204.305/2024, E-26/203.644/2024, E-26/200.530/2025/Fundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro/ GR - 001/Brazilian Federal Agency for Support and Evaluation of Graduate Education/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701800 OTO - NOTNLM OT - Atlantic Forest OT - conservation status OT - species delimitation COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:33 PHST- 2026/08/13 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - biology15201829 [pii] AID - biology-15-01829 [pii] AID - 10.3390/biology15201829 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 13;15(20):1829. doi: 10.3390/biology15201829. PMID- 42939429 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 13 TI - Transient Pulses and Transcriptomic Rigidity: Dynamic miRNA Expression Patterns in Ovine Ovarian Development Reveal Molecular Strategies Underlying Seasonal Reproduction. LID - 10.3390/biology15201828 [doi] LID - 1828 AB - Ovine fecundity is a key economic trait constraining livestock production efficiency, and the marked differences in reproductive strategies among breeds provide an ideal model for dissecting the molecular mechanisms underlying seasonal reproduction and prolificacy. In this study, we investigated Ujumqin sheep, a typical seasonally breeding, single-litter breed, by collecting ovarian tissues at three critical developmental stages-3 months (pre-pubertal), 6 months (puberty initiation), and 12 months (sexual maturity)-and performed whole-transcriptome sequencing to systematically characterize the dynamic expression trajectories of miRNAs, lncRNAs, circRNAs and mRNA during ovarian development. Unexpectedly, we found that miRNA expression changes did not follow a monotonic linear trajectory, but rather exhibited a highly nonlinear "transient pulse" pattern: pairwise comparisons revealed 42 and 44 differentially expressed miRNAs (DEMs) between 3 vs. 6 months and 6 vs. 12 months, respectively, yet only 21 DEMs between 3 vs. 12 months-an "inverted-V" distribution. Cross-validation by Mfuzz temporal clustering and Venn diagram intersection analyses pinpointed 10 core miRNAs that synchronously elevated during the puberty initiation window (3 --> 6 months) and returned to baseline by sexual maturity (6 --> 12 months). Target gene enrichment of these 10 miRNAs converged significantly in Wnt, cAMP, FoxO, and oxytocin signaling pathways-suggesting that these miRNAs are associated with molecular programs related to follicular development and hormone signaling at the 6-month puberty initiation window. We further constructed a competing endogenous RNA (ceRNA) regulatory network centered on these 10 core miRNAs and resolved two potential functional axes, lncRNA MSTRG.20588-miR-211-CTNNB1 and circRNA3387-miR-320d-ATG7, directly linking the transient pulse signals to follicle dominance selection and granulosa cell quality control. An exploratory Cross-breed comparison revealed that the prolific breed Small-Tailed Han sheep exhibited 227 DEMs at the puberty initiation window (approximately 5.4-fold more than Ujumqin sheep), with the early changes largely sustained into adulthood. In contrast, the majority of differential changes observed in Ujumqin sheep at 6 months were reversed and essentially "reset" by 12 months. Based on these findings, we propose a conceptual framework of "transcriptomic rigidity" versus "transcriptomic plasticity" to distinguish the two breeds: Ujumqin sheep ovaries tend to reset the transcriptome to a quiescent state after completing puberty initiation-a "rigidity" strategy that may represent the molecular hallmark of seasonal breeding breeds. Collectively, this study provides new conceptual tools for understanding the molecular regulation of seasonal reproduction in sheep and offers potential molecular targets for genetic improvement of reproductive performance in single-litter breeds. FAU - Tian, Ruchao AU - Tian R AD - College of Life Science, Jilin Normal University, Siping 136000, China. FAU - Lv, Yingyue AU - Lv Y AD - College of Life Science, Jilin Normal University, Siping 136000, China. FAU - Chen, Yuqian AU - Chen Y AD - College of Life Science, Jilin Normal University, Siping 136000, China. FAU - Wang, Zhidan AU - Wang Z AD - College of Life Science, Jilin Normal University, Siping 136000, China. FAU - Wang, Anqi AU - Wang A AD - College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China. FAU - Gu, Bo AU - Gu B AUID- ORCID: 0000-0003-4281-1621 AD - College of Life Science, Jilin Normal University, Siping 136000, China. FAU - Jiang, Huaizhi AU - Jiang H AD - College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China. LA - eng GR - 2021YFF1000702/National Key Research and Development Program of China/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701545 OTO - NOTNLM OT - Ujumqin sheep OT - ceRNA OT - miRNA OT - ovarian development OT - transient pulse pattern COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:33 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - biology15201828 [pii] AID - biology-15-01828 [pii] AID - 10.3390/biology15201828 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 13;15(20):1828. doi: 10.3390/biology15201828. PMID- 42939423 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - Whole-Genome Resequencing Reveals Genomic Signatures Underlying High-Altitude Adaptation in Ethiopian Indigenous Cattle. LID - 10.3390/biology15201822 [doi] LID - 1822 AB - High-altitude environments impose severe physiological challenges on mammals, yet the genetic basis of adaptation in Ethiopian indigenous cattle remains poorly understood. Here, we analyzed whole-genome resequencing data from 70 Ethiopian indigenous cattle representing seven breeds (Abigar, Barka, Boran, Felata, Fogera, Gojjam-Highland, and Horro) distributed across elevations ranging from 523 to 3410 m, obtained from the NCBI Sequence Read Archive. After alignment to the ARS-UCD2.0 reference genome, we identified 49,196,624 high-confidence SNPs and performed population genomic analyses (PCA, phylogenetic reconstruction, and ADMIXTURE), along with genome-wide selection scans using FST and nucleotide diversity (thetapi), and in silico virtual knockout of candidate genes in bovine lung single-cell transcriptomics. Population structure analyses revealed marked genetic differentiation between low- and mid-to-high-altitude populations, with substantial genetic connectivity and shared ancestry among high-altitude groups. We identified 398 candidate selective regions showing concordant FST and thetapi signals, encompassing 1816 candidate genes, significantly enriched in hypoxia-related and vascular remodeling pathways (phospholipase D, Ras, ErbB, Wnt, Hedgehog). Two hub candidate genes, PIK3CA and RHOA, were prioritized; virtual perturbation of PIK3CA altered extracellular matrix, focal adhesion, and integrin networks, whereas RHOA perturbation activated immune and inflammatory programs (NF-kappaB, phagocytosis, chemokine pathways) with upregulation of TYROBP, IL1B, TLR4, and NLRP3. Our findings indicate that Ethiopian cattle have evolved distinct genomic signatures associated with adaptation to chronic high-altitude environments, in which PIK3CA and RHOA may represent candidate regulators associated with extracellular matrix organization and immune-related transcriptional regulation, respectively, thereby providing new insights into mammalian adaptation to extreme environments. FAU - Ma, Ruoshan AU - Ma R AUID- ORCID: 0009-0008-8451-7045 AD - Yunnan Provincial Key Laboratory of Animal Science and Feed, Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China. FAU - Li, Dongfang AU - Li D AD - Yunnan Provincial Key Laboratory of Animal Science and Feed, Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China. FAU - Lu, Ying AU - Lu Y AUID- ORCID: 0009-0002-6056-3246 AD - Yunnan Provincial Key Laboratory of Animal Science and Feed, Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China. FAU - Wang, Bo AU - Wang B AD - Yunnan Provincial Key Laboratory of Animal Science and Feed, Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China. FAU - Deng, Weidong AU - Deng W AUID- ORCID: 0000-0002-9620-2082 AD - Yunnan Provincial Key Laboratory of Animal Science and Feed, Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China. FAU - Gao, Zhendong AU - Gao Z AD - Yunnan Provincial Key Laboratory of Animal Science and Feed, Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China. LA - eng GR - 202601BC070001; 202501AS070040/Program of Yunnan Fundamental Research Projects/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701653 OTO - NOTNLM OT - Ethiopian cattle OT - high-altitude adaptation OT - single-cell virtual knockout OT - whole-genome resequencing COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:33 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - biology15201822 [pii] AID - biology-15-01822 [pii] AID - 10.3390/biology15201822 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 12;15(20):1822. doi: 10.3390/biology15201822. PMID- 42939422 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - Cigarette Smoke Extract Impairs Regeneration and Tissue Homeostasis in the Planarian Dugesia japonica and May Be Associated with Changes in MAPK-Related Signaling. LID - 10.3390/biology15201821 [doi] LID - 1821 AB - Cigarette smoke extract (CSE) is used to assess tobacco toxicity, but its effects on freshwater planarian regeneration remain incompletely characterized. We investigated CSE toxicity in Dugesia japonica using acute toxicity tests, phenotypic observation, biochemical assays, immunofluorescence, TUNEL staining, and transcriptomic and proteomic analyses. The 96 h median lethal concentration (LC(50)) was 8.55% under the study-specific CSE definition. Exposure to 1% and 5% CSE impaired regeneration, with mortality and morphological abnormalities in the 5% group. CSE exposure also altered antioxidant and cholinergic enzyme activities, increased reactive oxygen species (ROS)-related fluorescence and malondialdehyde (MDA) content, and delayed neural remodeling. Fewer H3P-positive mitotic cells and more TUNEL-positive cells were observed, together with changes in proliferation- and apoptosis-related transcripts. Transcriptomic and proteomic analyses identified molecular responses involving stress, metabolism, and cellular homeostasis, although their overall concordance was low. Selected expression changes suggested mitogen-activated protein kinase (MAPK)-related signaling as a candidate response, but pathway activation and causal involvement were not established. These findings characterize regeneration impairment and associated cellular and molecular responses under laboratory CSE exposure conditions. They do not establish ecological risk at environmental exposure levels. FAU - Zhao, Zelong AU - Zhao Z AD - Zibo Normal College, Zibo 255100, China. AD - School of Life and Medicine, Shandong University of Technology, Zibo 255049, China. FAU - Wang, Ende AU - Wang E AD - School of Life and Medicine, Shandong University of Technology, Zibo 255049, China. FAU - Yang, Kexin AU - Yang K AD - School of Life and Medicine, Shandong University of Technology, Zibo 255049, China. FAU - Zhang, Chunmei AU - Zhang C AUID- ORCID: 0009-0008-7446-4664 AD - School of Life and Medicine, Shandong University of Technology, Zibo 255049, China. FAU - Song, Linxia AU - Song L AD - School of Life and Medicine, Shandong University of Technology, Zibo 255049, China. FAU - Xu, Zhenbiao AU - Xu Z AUID- ORCID: 0009-0004-4888-595X AD - School of Life and Medicine, Shandong University of Technology, Zibo 255049, China. LA - eng GR - 31550005, 31350004/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701927 OTO - NOTNLM OT - Dugesia japonica OT - MAPK signaling pathway OT - cigarette smoke extract OT - multi-omics analysis OT - oxidative stress OT - regeneration COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:33 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - biology15201821 [pii] AID - biology-15-01821 [pii] AID - 10.3390/biology15201821 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 12;15(20):1821. doi: 10.3390/biology15201821. PMID- 42939421 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 12 TI - Multi-Dimensional Characterization of Tetraspanins Reveals TSPAN6 as a Candidate Prognostic Hub in Kidney Renal Clear Cell Carcinoma. LID - 10.3390/biology15201820 [doi] LID - 1820 AB - Kidney renal clear cell carcinoma (KIRC) is a major subtype of renal cell carcinoma with substantial molecular heterogeneity and variable clinical outcomes. Tetraspanins (TSPANs) are membrane proteins involved in cell adhesion, signaling, migration, tumor progression, and interactions with the tumor microenvironment (TME); however, their collective significance in KIRC remains insufficiently characterized. The expression profile of the TSPAN gene family was evaluated in the TCGA-KIRC cohort using GEPIA2. Differentially expressed TSPANs were further investigated through protein-protein interaction network (PPIN) and univariate survival analyses. Prognostically significant TSPANs were assessed for genomic alterations, promoter methylation, and associations with tumor-infiltrating immune cells. Feed-forward-loop (FFL) was constructed and visualized using Cytoscape. Thereafter, the findings were evaluated in an independent KIRC dataset. Several TSPANs displayed significant differential expression between KIRC and normal renal tissues. TSPAN1 and TSPAN6 were significantly associated with overall survival (OS), with higher expression corresponding to more favorable outcomes. Notably, higher TSPAN6 expression was associated with improved OS but a higher estimated risk of relapse indicating an endpoint-dependent and potentially complex prognostic relationship. Promoter methylation of TSPAN1 and TSPAN6 showed associations with selected clinicopathological characteristics, while both genes demonstrated significant relationships with specific immune-cell populations. Network analysis identified a ZNF260-miR-6794-5p-TSPAN6 FFL subnetwork motif, prioritizing TSPAN6 as the hub TSPAN. TSPAN6 was additionally detected among differentially expressed genes (DEGs) in an independent GEO cohort. This study identifies TSPAN6 as a promising candidate biomarker in KIRC and provides a multi-dimensional framework linking TSPAN dysregulation with prognosis, epigenetic regulation, immune infiltration, and regulatory networks. Experimental and clinical validation is warranted. FAU - Alharbi, Hajed Obaid A AU - Alharbi HOA AUID- ORCID: 0000-0003-4566-2932 AD - Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia. FAU - Sarwar, Tarique AU - Sarwar T AUID- ORCID: 0000-0003-2811-220X AD - Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia. FAU - Rahmani, Arshad Husain AU - Rahmani AH AD - Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia. LA - eng PT - Journal Article DEP - 20261012 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701831 OTO - NOTNLM OT - FFL OT - KIRC OT - PPIN OT - TSPAN6 OT - prognosis OT - prognostic biomarker COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:33 PHST- 2026/09/06 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - biology15201820 [pii] AID - biology-15-01820 [pii] AID - 10.3390/biology15201820 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 12;15(20):1820. doi: 10.3390/biology15201820. PMID- 42939411 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 10 TI - Beyond the Liver: Brain Rap1 Signaling in Metformin's Antidiabetic Action. LID - 10.3390/biology15201810 [doi] LID - 1810 AB - Metformin remains a promising therapeutic drug for type 2 diabetes mellitus (T2DM) due to its glucose-lowering effects through hepatic and other peripheral mechanisms. Recent evidence shows that the brain may represent an additional site of action. The small GTPase Ras-related protein 1 (Rap1), potentially connects central metformin exposure to systemic glucose homeostasis. The current review discusses neuronal Rap1 regulation and a role in metformin's antidiabetic effects. Preclinical findings indicate that excessive neuronal Rap1 activity may contribute to metabolic dysfunction, whereas its inhibition is related to improved glucose regulation. Recent experimental evidence further suggests that low-dose metformin reduces Rap1 activity in the ventromedial hypothalamus (VMH). Moreover, metformin also alters the excitability of steroidogenic factor-1-positive neurons that contributes to its hypoglycemic effect. Initial findings also suggest a functional relationship between Rap1 and AMPK, although the molecular mechanisms showing the mTORC1 connection remain to be clarified. This review also considers how central Rap1 signaling might interact with gut-brain-liver pathways that can affect peripheral metabolism. Human central nervous system (CNS) studies and primary RAP1A pharmacogenetic results offer indirect translational support, but direct evidence of hypothalamic Rap1 involvement in patients is currently not documented. Extending beyond previous reviews of the preclinical VMH-Rap1 mechanism, this review integrates dose-CNS exposure relationships with emerging human evidence, particularly CSF pharmacokinetics and RAP1A pharmacogenetics. These findings provide preliminary translational links, although direct hypothalamic Rap1 engagement in humans remains unproven. Future studies should elucidate circuit-specific mechanisms, determine reliable biomarkers, and assess selective pathway modulation. A better understanding of this relationship may help determine whether brain Rap1 could ultimately serve as a therapeutic target to improve metformin responsiveness in T2DM. FAU - Khan, Masood Alam AU - Khan MA AUID- ORCID: 0000-0003-4031-4189 AD - Department of Basic Health Sciences, College of Applied Medical Sciences, Qassim University, Buraydah 51452, Saudi Arabia. FAU - Khan, Mohammad Hamza AU - Khan MH AD - Jawaharlal Nehru Medical College and Hospital (JNMCH), Aligarh 202002, India. LA - eng PT - Journal Article PT - Review DEP - 20261010 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701204 OTO - NOTNLM OT - Rap1 signaling OT - brain OT - diabetes mellitus OT - mTOR OT - metformin OT - neural networks COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:33 PHST- 2026/09/13 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - biology15201810 [pii] AID - biology-15-01810 [pii] AID - 10.3390/biology15201810 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 10;15(20):1810. doi: 10.3390/biology15201810. PMID- 42939409 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 10 TI - Comprehensive Bioinformatic Analysis and Expression Profiling of the Alcohol Dehydrogenase (ADH) Gene Family in Morus alba. LID - 10.3390/biology15201808 [doi] LID - 1808 AB - Alcohol dehydrogenase (ADH) is a key enzyme for ethanol fermentation under anaerobiosis and is vital for plant growth, development, and abiotic stress tolerance. Although the (MDR)-ADH gene family in Morus has not been systematically characterized, this study performs a genome-wide analysis to investigate its evolution and flooding-responsive transcription patterns. In this study, a comprehensive genome-wide bioinformatic analysis of the Morus alba (MDR)-ADH (MaADH) gene family, including physicochemical characteristics, evolutionary relationships, subcellular localization, gene structural organization, cis-regulatory element composition, and interspecific syntenic relationships, was conducted. A total of 20 MaADH genes were identified from the M. alba genome, and all encoded proteins contained the conserved GroES-like alcohol dehydrogenase N-terminal domain (ADH_N) and zinc-binding alcohol dehydrogenase domain (ADH_zinc_N). Phylogenetic analysis grouped these genes into six evolutionary clades. The identified MaADH genes showed an uneven chromosomal distribution across seven chromosomes. Moreover, qRT-PCR analysis of 11 selected MaADH genes showed that most genes are significantly upregulated in the roots and stems under flooding conditions, with a characteristic temporal expression pattern of initial induction followed by attenuation. These results suggest that the differentially expressed MaADH genes may participate in the transcriptional response to flooding stress in M. alba. These results will contribute to further research on the biological function of the MaADH candidates and provide new candidate genes for breeding programs focused on the flood response of M. alba. FAU - Tang, Ju AU - Tang J AUID- ORCID: 0009-0004-6981-9185 AD - College of Biology and Food Engineering, Chongqing Sanxia University of Science and Technology, Chongqing 404100, China. FAU - Xie, Mingyue AU - Xie M AD - College of Biology and Food Engineering, Chongqing Sanxia University of Science and Technology, Chongqing 404100, China. FAU - Zhou, Minxiao AU - Zhou M AD - College of Biology and Food Engineering, Chongqing Sanxia University of Science and Technology, Chongqing 404100, China. FAU - Zhang, Shenghao AU - Zhang S AD - College of Biology and Food Engineering, Chongqing Sanxia University of Science and Technology, Chongqing 404100, China. FAU - Li, Yanjie AU - Li Y AD - College of Biology and Food Engineering, Chongqing Sanxia University of Science and Technology, Chongqing 404100, China. FAU - Hu, Jingtao AU - Hu J AUID- ORCID: 0000-0002-8880-5887 AD - College of Biology and Food Engineering, Chongqing Sanxia University of Science and Technology, Chongqing 404100, China. LA - eng GR - CSTB2024NSCQ-LZX0004/Natural Science Foundation of Chongqing, China/ GR - YJSKY26057/Chongqing Sanxia University of Science and Technology/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13702005 OTO - NOTNLM OT - Morus alba OT - abiotic stress OT - alcohol dehydrogenase (ADH) OT - gene expression OT - genome-wide analysis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:33 PHST- 2026/08/28 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - biology15201808 [pii] AID - biology-15-01808 [pii] AID - 10.3390/biology15201808 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 10;15(20):1808. doi: 10.3390/biology15201808. PMID- 42939405 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 9 TI - Metabolomics Reveals Differences in Muscle Metabolite Profiles Between Daweishan Mini Chickens and Tegel Broilers at Marketable Age. LID - 10.3390/biology15201804 [doi] LID - 1804 AB - Metabolic differences are an important factor influencing the quality of chicken meat. To investigate the differences in muscle flavor precursors between Daweishan mini chickens and Tegel broilers, integrated metabolomics analyses were used to compare the muscle metabolite profiles of the two breeds at marketable age (150-day-old Daweishan mini chickens and 45-day-old Tegel broilers). The differential metabolites (DMs) were significantly enriched in aminoacyl-tRNA biosynthesis; glycine, serine and threonine metabolism; glutathione metabolism; alanine, aspartate and glutamate metabolism; arginine biosynthesis; purine metabolism; pantothenate and CoA biosynthesis; cysteine and methionine metabolism; glycerophospholipid metabolism; and D-glutamine and D-glutamate metabolism, which contributed to the quality differences between the two breeds. Moreover, 3-methylhistidine, L-carnosine, L-anserine, DL-lactate, choline, L-phenylalanine, and L-ascorbic acid were present at higher relative abundances in the muscle of Daweishan mini chickens at marketable age. This study provides basic data on the mechanisms underlying the meat quality of local chickens and a reference for the development and utilization of local chicken breeds and the selective breeding of high-quality broilers. FAU - Huang, Wei AU - Huang W AUID- ORCID: 0009-0008-1624-4794 AD - College of Agronomy and Life Sciences, Kunming University, Kunming 650214, China. AD - Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China. FAU - Ran, Jinshan AU - Ran J AD - College of Agronomy and Life Sciences, Kunming University, Kunming 650214, China. FAU - Wu, Shangwen AU - Wu S AD - College of Agronomy and Life Sciences, Kunming University, Kunming 650214, China. FAU - Li, Jing AU - Li J AD - College of Agronomy and Life Sciences, Kunming University, Kunming 650214, China. FAU - Ning, Tiao AU - Ning T AUID- ORCID: 0009-0004-5953-4637 AD - College of Agronomy and Life Sciences, Kunming University, Kunming 650214, China. FAU - Du, Yanli AU - Du Y AD - College of Agronomy and Life Sciences, Kunming University, Kunming 650214, China. FAU - Yang, Xiujuan AU - Yang X AD - Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China. LA - eng GR - 202501BA070001-074/Yunnan Province Science and Technology Department/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701208 OTO - NOTNLM OT - Daweishan mini chicken OT - Tegel broiler OT - differential metabolites OT - meat quality OT - untargeted metabolomics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:33 PHST- 2026/09/04 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - biology15201804 [pii] AID - biology-15-01804 [pii] AID - 10.3390/biology15201804 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 9;15(20):1804. doi: 10.3390/biology15201804. PMID- 42939401 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 9 TI - Wheat VPE Family: Identification, Evolution and Expression Associated with Grain Colour. LID - 10.3390/biology15201800 [doi] LID - 1800 AB - Vacuolar processing enzymes (VPEs) are legumain-type cysteine proteases regulating plant protein processing, programmed cell death, seed maturation and stress responses. Nevertheless, the VPE family remains poorly characterized in hexaploid wheat (Triticum aestivum L.), and its possible roles in grain pigmentation are unclear. Here, we genome-wide identified 27 TaVPE genes and performed multi-omics analyses. Phylogenetic classification separated TaVPE proteins into four clades, all possessing conserved Peptidase_C13 and legumain_C domains, with similar motif patterns within each clade. These 27 TaVPE genes were unevenly located across 12 chromosomes of homoeologous groups 2, 3, 5 and 6. Collinearity analysis uncovered 26 intra-wheat collinear blocks and 47 orthologous gene pairs between wheat and rice, maize and foxtail millet, while no collinear counterparts were found in Arabidopsis. All duplicated pairs had Ka/Ks values less than 1, suggesting strong purifying selection. Numerous cis-acting elements related to hormones (ABA, MeJA, SA), stresses, and MYB/bHLH transcription factor binding sites were detected in TaVPE promoters. Expression data from public databases and transcriptomes of purple-grained Donghei-1 and white-grained Jinmai-66 (17, 31, 36 and 41 days after flowering) demonstrated predominant TaVPE expression at anthesis and grain-filling stages. Five TaVPE genes were retained as candidate genes associated with purple grain colour: TaVPE6, TaVPE15, TaVPE18, TaVPE25 and TaVPE26. All five showed significantly higher expression in the purple-grained cultivar than in the white-grained one at one or more mid-to-late grain-filling stages. This work establishes the first systematic profile of wheat VPE family and identifies candidate genes potentially associated with anthocyanin accumulation, supporting future functional research and molecular breeding for anthocyanin-enriched wheat. FAU - Wang, Yanzhen AU - Wang Y AUID- ORCID: 0000-0002-7532-2990 AD - Center for Agricultural Genetic Resources Research, Shanxi Agricultural University, Taiyuan 030031, China. FAU - Wang, Yanqi AU - Wang Y AD - Center for Agricultural Genetic Resources Research, Shanxi Agricultural University, Taiyuan 030031, China. AD - College of Agriculture, Shanxi Agricultural University (Institute of Crop Sciences), Taigu 030801, China. FAU - Zhao, Jiangtao AU - Zhao J AD - Seed Industry Development Center of Shanxi Province, Taiyuan 030006, China. FAU - Zheng, Jun AU - Zheng J AD - Zuoquan Modern Agricultural Industry Development Service Center, Zuoquan 032600, China. FAU - Lei, Menglin AU - Lei M AD - Center for Agricultural Genetic Resources Research, Shanxi Agricultural University, Taiyuan 030031, China. FAU - Guo, Ganggang AU - Guo G AUID- ORCID: 0000-0001-9970-5382 AD - State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. FAU - Liu, Xia AU - Liu X AD - Center for Agricultural Genetic Resources Research, Shanxi Agricultural University, Taiyuan 030031, China. LA - eng GR - CXGC202446/Shanxi Agricultural University/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701537 OTO - NOTNLM OT - Triticum aestivum OT - grain colour OT - synteny OT - vacuolar processing enzyme OT - whole-genome duplication COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:33 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - biology15201800 [pii] AID - biology-15-01800 [pii] AID - 10.3390/biology15201800 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 9;15(20):1800. doi: 10.3390/biology15201800. PMID- 42939395 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 9 TI - Effects of Different Formulations of Exogenous Nutrient Bag Waste on Soil Properties and Physiological Activities and Root Transcriptomic Responses of Oat. LID - 10.3390/biology15201794 [doi] LID - 1794 AB - This study used typical chestnut soil from Huangyuan County, Qinghai Province as the experimental soil, and selected three oat varieties, "Baiyan 7", "Qingyan 2", and "Qinghai 444", for mixed sowing. A blank control (CK) and five treatments with different ratios of exogenous nutrient bag waste (PF-R-1 to PF-R-5) were established. Through soil physicochemical analysis, microbial biomass determination, RNA sequencing (RNA-Seq), and weighted gene co-expression network analysis (WGCNA), the effects of exogenous nutrient bag waste with different ratios soil nutrient availability, microbial activity and root gene expression in oats were systematically evaluated. The results showed that most treatments enhanced soil organic matter, nitrogen content, and microbial activity. Transcriptomic analysis revealed that the comparison between PF-R-3 and PF-R-2 treatments exhibited the highest total number of differentially expressed genes (DEGs), with enrichment in pathways related to ribosomal protein translation, oxidative phosphorylation energy metabolism, and DNA damage repair. These enrichment patterns suggest potential changes in energy metabolism and protein synthesis, although direct functional validation was not performed. Moreover, DEGs uniquely identified in PF-R-3 were significantly enriched in nitrogen metabolism and secondary metabolite biosynthesis pathways; WGCNA identified the coral2 module as the core regulatory module, with its genes predominantly enriched in functions related to transmembrane transport and plant hormone signal transduction, which were highly consistent with efficient root nutrient acquisition and the maintenance of metabolic homeostasis. Fluorescence staining confirmed the successful colonization of oat root tissues by Morchella mycelium. Taken together, based on the integration of soil nutrient supply stability, root gene expression regulation, and fungal colonization evidence, the PF-R-3 treatment (wheat + corncob + oat) emerged as a promising candidate among the tested formulations under the experimental conditions. However, further field trials incorporating yield and quality data are needed before a definitive recommendation can be made. This study provides a theoretical basis and technical support for the resource utilization of exogenous nutrient bag waste and the green cultivation of oat in alpine regions. FAU - Gong, Li AU - Gong L AD - Research Institute of Science and Technology, Qinghai University, Xining 810016, China. AD - Key Laboratory of Adaptive Management for Alpine Grassland Ecosystem of Qinghai Province, Qinghai University, Xining 810016, China. FAU - Wang, Le AU - Wang L AD - Research Institute of Science and Technology, Qinghai University, Xining 810016, China. FAU - Su, Liping AU - Su L AD - Research Institute of Science and Technology, Qinghai University, Xining 810016, China. FAU - Sa, Wei AU - Sa W AD - Research Institute of Science and Technology, Qinghai University, Xining 810016, China. FAU - Dong, Quanmin AU - Dong Q AUID- ORCID: 0000-0001-6178-373X AD - Research Institute of Science and Technology, Qinghai University, Xining 810016, China. AD - Key Laboratory of Adaptive Management for Alpine Grassland Ecosystem of Qinghai Province, Qinghai University, Xining 810016, China. LA - eng GR - 2026-NK-Z18/Department of Science and Technology of Qinghai Province/ GR - 2025-GPKY-25/Qinghai University/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701462 OTO - NOTNLM OT - WGCNA OT - exogenous nutrient bag OT - oat OT - transcriptome COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:33 PHST- 2026/08/20 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - biology15201794 [pii] AID - biology-15-01794 [pii] AID - 10.3390/biology15201794 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 9;15(20):1794. doi: 10.3390/biology15201794. PMID- 42939394 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 9 TI - An In Vitro Feline Macrophage Model as a Tool to Study Leishmania Infection and Glucuronoxylomannan-Mediated Modulation. LID - 10.3390/biology15201792 [doi] LID - 1792 AB - Feline leishmaniasis is an emerging zoonotic disease, yet the cellular and immunological mechanisms underlying Leishmania infection in cats remain poorly understood. In this study, we established and characterized Fcwf-4 feline macrophages as an in vitro model of Leishmania amazonensis infection and investigated the immunomodulatory effects of glucuronoxylomannan (GXM), the major capsular polysaccharide of Cryptococcus neoformans. Fcwf-4 macrophages were infected with L. amazonensis promastigotes and evaluated at 2, 24, and 48 h post-infection. Parasite burden, macrophage viability, nitric oxide (NO), and reactive oxygen species (ROS) production were assessed in the presence or absence of GXM. Fcwf-4 macrophages supported L. amazonensis infection throughout the experimental period, with a progressive decrease in the infection index, indicating partial control of parasite burden over time. Notably, the presence of GXM increased the infection index without compromising macrophage viability, suggesting that enhanced parasite persistence resulted from immunomodulation rather than cytotoxicity. LPS stimulation enhanced NO and ROS production, whereas L. amazonensis infection selectively reduced NO production, indicating impairment of macrophage effector responses. Further, GXM suppressed ROS production, including basal ROS levels, suggesting an additional inhibitory effect on macrophage oxidative responses. Collectively, these findings provide experimental evidence that Fcwf-4 could be useful as a tool to study feline macrophage-Leishmania interactions in vitro and co-infection-associated immunomodulation. FAU - Pepino, Fernanda de Paula AU - Pepino FP AD - Departamento de Microbiologia e Imunologia Veterinaria, Instituto de Veterinaria, Universidade Federal Rural do Rio de Janeiro, Seropedica 23897-000, RJ, Brazil. FAU - Figueirinha, Ana Carolina AU - Figueirinha AC AD - Departamento de Microbiologia e Imunologia Veterinaria, Instituto de Veterinaria, Universidade Federal Rural do Rio de Janeiro, Seropedica 23897-000, RJ, Brazil. FAU - Dos Santos, Renata Quintanilha AU - Dos Santos RQ AUID- ORCID: 0009-0008-5719-8843 AD - Departamento de Microbiologia e Imunologia Veterinaria, Instituto de Veterinaria, Universidade Federal Rural do Rio de Janeiro, Seropedica 23897-000, RJ, Brazil. FAU - Borges, Joyce de Meyrelles AU - Borges JM AD - Departamento de Microbiologia e Imunologia Veterinaria, Instituto de Veterinaria, Universidade Federal Rural do Rio de Janeiro, Seropedica 23897-000, RJ, Brazil. AD - Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil. FAU - da Silva-Junior, Elias Barbosa AU - da Silva-Junior EB AUID- ORCID: 0000-0001-7953-6948 AD - Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil. FAU - Previato, Jose Osvaldo AU - Previato JO AD - Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil. FAU - Mendonca-Previato, Lucia AU - Mendonca-Previato L AUID- ORCID: 0000-0003-3927-3244 AD - Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil. FAU - Nascimento, Danielle de Oliveira AU - Nascimento DO AD - Departamento de Microbiologia e Imunologia Veterinaria, Instituto de Veterinaria, Universidade Federal Rural do Rio de Janeiro, Seropedica 23897-000, RJ, Brazil. FAU - Freire-de-Lima, Celio Geraldo AU - Freire-de-Lima CG AUID- ORCID: 0000-0002-4148-3657 AD - Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, RJ, Brazil. AD - Instituto Nacional de Ciencia e Tecnologia em Doencas Infecciosas e Cronicas das Mucosas e da Pele (INCT Mucosas e Pele), Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil. FAU - Decote-Ricardo, Debora AU - Decote-Ricardo D AUID- ORCID: 0000-0001-8761-7641 AD - Departamento de Microbiologia e Imunologia Veterinaria, Instituto de Veterinaria, Universidade Federal Rural do Rio de Janeiro, Seropedica 23897-000, RJ, Brazil. FAU - Pinto-da-Silva, Lucia Helena AU - Pinto-da-Silva LH AUID- ORCID: 0000-0002-7085-8649 AD - Departamento de Microbiologia e Imunologia Veterinaria, Instituto de Veterinaria, Universidade Federal Rural do Rio de Janeiro, Seropedica 23897-000, RJ, Brazil. LA - eng GR - E-26/210.800/2025/Fundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiro/ GR - 408487/2025-9/Conselho Nacional de Desenvolvimento Cientifico e Tecnologico/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701880 OTO - NOTNLM OT - Fcwf-4 feline macrophages OT - Leishmania OT - glucuronoxylomannan (GXM) OT - immunomodulation OT - purified capsular polysaccharide COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:33 PHST- 2026/08/24 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - biology15201792 [pii] AID - biology-15-01792 [pii] AID - 10.3390/biology15201792 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 9;15(20):1792. doi: 10.3390/biology15201792. PMID- 42939393 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2079-7737 (Print) IS - 2079-7737 (Electronic) IS - 2079-7737 (Linking) VI - 15 IP - 20 DP - 2026 Oct 9 TI - Analysis of Spatiotemporal Changes in Jasmonic Acid, Salicylic Acid, and Indole-3-Acetic Acid in Tea Plants Based on Silylation and Gas Chromatography-Mass Spectrometry. LID - 10.3390/biology15201793 [doi] LID - 1793 AB - Phytohormones play a vital role in plant responses to biotic stresses. Although phytohormone determination mainly employs ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), its high costs and technical requirements limit utility, whereas gas chromatography-mass spectrometry (GC-MS) is more widely used. To determine jasmonic acid (JA), salicylic acid (SA), and indole-3-acetic acid (IAA) in tea plants, a GC-MS method was developed, including selection of silylating reagents and establishment of metabolism quenching and purification methods. SA, JA, and IAA contents in tea leaves increased significantly after storage at -80 degrees C or extraction at room temperature. These increases were prevented by immersing samples in a 95 degrees C water bath. Quantification limits for SA, JA, and IAA were 0.020 ng mg(-1), 0.015 ng g(-1), and 0.015 ng g(-1), similar to or exceeding those obtained by UHPLC-MS/MS. Recoveries and RSD were 93.1-98.9% and 2.1-9.9%, respectively. Only 10 mg of tea leaves was sufficient for simultaneous analysis of the three phytohormones. The JA response to geometrids was strong compared with the SA response, whereas the opposite trend was observed for leafhoppers. The systemic responses of JA and SA were rapid, similar to the local response. These results indicate that the proposed method is sensitive and reliable. FAU - Sun, Chenqi AU - Sun C AD - Key Laboratory of Soybean Diseases and Pests Control in Ministry of Agriculture and Rural Affairs, College of Plant Protection, Jilin Agricultural University, Changchun 130118, China. AD - Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou 310008, China. FAU - Hu, Kunzhu AU - Hu K AD - Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou 310008, China. FAU - Bian, Lei AU - Bian L AD - Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou 310008, China. FAU - Luo, Zongxiu AU - Luo Z AD - Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou 310008, China. FAU - Li, Zhaoqun AU - Li Z AD - Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou 310008, China. FAU - Xiu, Chunli AU - Xiu C AUID- ORCID: 0000-0002-6001-9139 AD - Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou 310008, China. FAU - Fu, Nanxia AU - Fu N AUID- ORCID: 0000-0001-6151-9808 AD - Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou 310008, China. FAU - Chen, Zongmao AU - Chen Z AD - Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou 310008, China. FAU - Gao, Yu AU - Gao Y AUID- ORCID: 0000-0002-3369-8578 AD - Key Laboratory of Soybean Diseases and Pests Control in Ministry of Agriculture and Rural Affairs, College of Plant Protection, Jilin Agricultural University, Changchun 130118, China. FAU - Cai, Xiaoming AU - Cai X AUID- ORCID: 0000-0003-1535-8761 AD - Key Laboratory of Biology, Genetics and Breeding of Special Economic Animals and Plants, Ministry of Agriculture and Rural Affairs, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou 310008, China. LA - eng GR - LY24C140002/Zhejiang Provincial Natural Science Foundation/ GR - CARS-18/Modern Agricultural Industry Technology System/ GR - ZDYF2024XDNY245/Key Research and Development Program of Hainan province/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Biology (Basel) JT - Biology JID - 101587988 PMC - PMC13701311 OTO - NOTNLM OT - gas chromatography-mass spectrometry OT - indole-3-acetic acid OT - jasmonic acid OT - metabolism quenching OT - salicylic acid OT - silylation derivatization OT - spatiotemporal change COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:33 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - biology15201793 [pii] AID - biology-15-01793 [pii] AID - 10.3390/biology15201793 [doi] PST - epublish SO - Biology (Basel). 2026 Oct 9;15(20):1793. doi: 10.3390/biology15201793. PMID- 42939390 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 21 TI - Current Video Gaming Is Associated with Visual-Psychomotor Efficiency and Visuomotor Coordination in Adolescents. LID - 10.3390/brainsci16101140 [doi] LID - 1140 AB - BACKGROUND/OBJECTIVES: Video gaming places repeated demands on rapid visual information processing, response selection, and visuomotor coordination. However, it remains unclear whether current gaming in adolescence is associated with broadly enhanced cognitive performance or with selective differences in visual-psychomotor functioning. This study examined associations between current video gaming and visual-psychomotor efficiency, visuomotor coordination, visual search, perceptual performance, and sequential-executive processing in adolescents. METHODS: This exploratory secondary cross-sectional reanalysis used an existing dataset of 120 adolescents aged 14-16 years (60 girls, 60 boys), including 92 current and 28 non-current players. Five principal outcomes were analysed using age- and sex-adjusted regression, HC3 standard errors, and Benjamini-Hochberg correction. Sensitivity analyses examined girls, school membership, and all 121 source records. RESULTS: Current gaming was associated with higher PiM (B = 6.92, 95% CI [1.24, 12.59], q = 0.043) and shorter Number Localization time (9.6%, q = 0.042) in the principal models. PiM estimates remained positive across school and sample sensitivity analyses, although not every leave-one-school-out model met the FDR threshold. Inference for visual-search time was more sensitive to analytical specification. The component analyses described overlapping measures underlying PiM. Intensity, tenure, and genre analyses were inconclusive after correction. CONCLUSIONS: Current gaming was associated most consistently with greater psychomotor throughput. Visual-search estimates generally favoured current players but were less stable across specifications. These exploratory cross-sectional findings do not establish a causal benefit, a general cognitive advantage, or an optimal gaming dose or genre. FAU - Zaleski, Grzegorz AU - Zaleski G AUID- ORCID: 0000-0001-6240-2935 AD - Faculty of Social Sciences and Computer Science, Academy WSB-NLU, 17 Grunwaldzka Street, 33-300 Nowy Sacz, Poland. FAU - Horoszkiewicz, Krzysztof AU - Horoszkiewicz K AUID- ORCID: 0000-0001-6719-256X AD - Faculty of Social Sciences and Computer Science, Academy WSB-NLU, 17 Grunwaldzka Street, 33-300 Nowy Sacz, Poland. FAU - Kopanska, Marta AU - Kopanska M AUID- ORCID: 0000-0003-3936-0698 AD - Department of Medical Communication and Professional Competency Development, Faculty of Medicine, Collegium Medicum, University of Rzeszow, 35-310 Rzeszow, Poland. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702617 OTO - NOTNLM OT - adolescents OT - cognitive performance OT - psychomotor performance OT - video gaming OT - visual attention OT - visuomotor coordination COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:33 PHST- 2026/09/26 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - brainsci16101140 [pii] AID - brainsci-16-01140 [pii] AID - 10.3390/brainsci16101140 [doi] PST - epublish SO - Brain Sci. 2026 Oct 21;16(10):1140. doi: 10.3390/brainsci16101140. PMID- 42939389 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 21 TI - A Comprehensive Narrative Review on Autonomic Nervous System Pathophysiology in Spinal Cord Injury and the Use of Invasive and Non-Invasive Electrical Stimulation for Recovery. LID - 10.3390/brainsci16101139 [doi] LID - 1139 AB - Spinal cord injury (SCI) significantly reduces both quality of life and life expectancy due to the loss of motor and sensory function, as well as its impact on autonomic dysfunction (AD). Complications related to AD include impaired sphincter control and thermoregulation, orthostatic hypotension, respiratory difficulties, sexual dysfunction, and gastrointestinal complications, among others. These complications limit physical rehabilitation capacities, exacerbating SCI-related challenges and increasing healthcare costs. Currently, no effective clinical treatments exist to restore impaired autonomic neural control. However, invasive and non-invasive neuromodulation strategies such as epidural spinal cord stimulation (eSCS) and transcutaneous spinal cord stimulation (tSCS), respectively, have demonstrated promising results in improving autonomic function. Despite these advances, drawing definitive conclusions remains challenging because studies vary in sample size, stimulation parameters, session numbers, and assessment methods. This narrative review outlines the primary consequences of SCI on the autonomic nervous system (ANS) and evaluates the effects of eSCS and tSCS on cardiovascular, respiratory, gastrointestinal, sphincter control, thermoregulation, and sexual function, while highlighting the diversity of approaches in current studies. FAU - Sanchez-Lastra, Karina Yazmin AU - Sanchez-Lastra KY AD - Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Toluca 50180, Estado de Mexico, Mexico. FAU - Attie-Harari, Rossy AU - Attie-Harari R AD - Facultad de Ciencias de la Salud, Universidad Anahuac Mexico, Huixquilucan 50180, Estado de Mexico, Mexico. FAU - Castillo-Cuadros, Maria Fernanda AU - Castillo-Cuadros MF AUID- ORCID: 0009-0004-1268-4750 AD - Facultad de Ciencias de la Salud, Universidad Anahuac Mexico, Huixquilucan 50180, Estado de Mexico, Mexico. FAU - Cuellar, Carlos A AU - Cuellar CA AUID- ORCID: 0000-0001-9404-3914 AD - School of Sport Sciences, Universidad Anahuac Mexico, Huixquilucan 50180, Estado de Mexico, Mexico. FAU - Pliego-Carrillo, Adriana Cristina AU - Pliego-Carrillo AC AUID- ORCID: 0000-0001-7435-7414 AD - Facultad de Medicina, Universidad Autonoma del Estado de Mexico, Toluca 50180, Estado de Mexico, Mexico. LA - eng GR - Universidad Anahuac Mexico/ PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702426 OTO - NOTNLM OT - autonomic dysfunction OT - autonomic nervous system OT - cardiovascular function OT - epidural stimulation OT - invasive neuromodulation OT - neuromodulation OT - non-invasive neuromodulation OT - respiratory function OT - spinal cord injury OT - transcutaneous spinal cord stimulation COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:33 PHST- 2026/08/22 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - brainsci16101139 [pii] AID - brainsci-16-01139 [pii] AID - 10.3390/brainsci16101139 [doi] PST - epublish SO - Brain Sci. 2026 Oct 21;16(10):1139. doi: 10.3390/brainsci16101139. PMID- 42939386 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 21 TI - Intraoperative Measurement of Internal Carotid Artery Blood Flow Using Transit-Time Flowmetry After Carotid Endarterectomy: Results from 196 Consecutive Cases. LID - 10.3390/brainsci16101136 [doi] LID - 1136 AB - Background/Objectives: Carotid endarterectomy (CEA) is a standard surgical treatment for high-grade symptomatic and asymptomatic internal carotid artery (ICA) stenosis. The Transit Time Flowmeter (TTF) provides an easy-to-use tool to quantify intraoperative ICA blood flow in mL/min. This study aimed to (1) describe post-CEA ICA flow quantified with TTF, (2) identify predictors of post-CEA ICA flow, and (3) examine its association with contralateral steno-occlusive disease. Methods: We queried data from our prospective registry of patients who underwent a CEA between January 2019 and July 2024 and had intraoperative registered post-CEA ICA flow measurements using TTF. Preoperative ICA stenosis was graded according to the NASCET criteria. The clinical data was retrospectively collected. Results: Data from 196 CEAs in 190 patients (median age 74 [67-81]; 25% female) were included. Most patients (86%) had a symptomatic ICA stenosis. The median post-CEA ICA flow was 170 mL/min [IQR 140-242; range 40-800]. Post-CEA ICA flow was not associated with age, sex, comorbidities, symptomatic stenosis, left ventricular ejection fraction (LVEF), or ipsilateral stenosis severity but was significantly higher in patients requiring intraluminal shunt placement during CEA (p = 0.010) as well as in those with contralateral steno-occlusive disease (p < 0.001). Conclusions: TTF is a practical intraoperative tool for quantitative flow assessment during CEA. Higher post-CEA ICA flow in patients with an intraoperative shunt placement or with contralateral steno-occlusive disease may reflect a compensatory hyperperfusion, as the ipsilateral ICA serves as a key supplier when collateral pathways are incomplete. FAU - Gonel, Meltem AU - Gonel M AUID- ORCID: 0000-0002-8652-0554 AD - Department of Neurosurgery, Clinical Neuroscience Center, University Hospital Zurich, 8091 Zurich, Switzerland. AD - Faculty of Medicine, University of Zurich, 8032 Zurich, Switzerland. FAU - Inauen, Corinne AU - Inauen C AUID- ORCID: 0000-0002-7786-2720 AD - Department of Neurology, Clinical Neuroscience Center, University Hospital Zurich, 8091 Zurich, Switzerland. FAU - Heesen, Philip AU - Heesen P AUID- ORCID: 0000-0002-5090-4935 AD - Department of Neurosurgery, Clinical Neuroscience Center, University Hospital Zurich, 8091 Zurich, Switzerland. AD - Faculty of Medicine, University of Zurich, 8032 Zurich, Switzerland. FAU - Sebok, Martina AU - Sebok M AUID- ORCID: 0000-0002-7246-3421 AD - Department of Neurosurgery, Clinical Neuroscience Center, University Hospital Zurich, 8091 Zurich, Switzerland. AD - Faculty of Medicine, University of Zurich, 8032 Zurich, Switzerland. FAU - Paruvacattu, Shreyes AU - Paruvacattu S AD - Department of Neurosurgery, Clinical Neuroscience Center, University Hospital Zurich, 8091 Zurich, Switzerland. AD - Faculty of Medicine, University of Zurich, 8032 Zurich, Switzerland. FAU - Schmidlechner, Tristan AU - Schmidlechner T AUID- ORCID: 0009-0006-0915-5983 AD - Department of Neurosurgery, Clinical Neuroscience Center, University Hospital Zurich, 8091 Zurich, Switzerland. AD - Faculty of Medicine, University of Zurich, 8032 Zurich, Switzerland. FAU - Colombo, Elisa AU - Colombo E AUID- ORCID: 0000-0003-0424-1250 AD - Department of Neurosurgery, Clinical Neuroscience Center, University Hospital Zurich, 8091 Zurich, Switzerland. AD - Faculty of Medicine, University of Zurich, 8032 Zurich, Switzerland. FAU - Percuoco, Veronica AU - Percuoco V AUID- ORCID: 0009-0006-6559-5481 AD - Department of Neurosurgery, Clinical Neuroscience Center, University Hospital Zurich, 8091 Zurich, Switzerland. FAU - Fierstra, Jorn AU - Fierstra J AUID- ORCID: 0000-0001-6220-0727 AD - Department of Neurosurgery, Clinical Neuroscience Center, University Hospital Zurich, 8091 Zurich, Switzerland. AD - Faculty of Medicine, University of Zurich, 8032 Zurich, Switzerland. FAU - Globas, Christoph AU - Globas C AUID- ORCID: 0000-0002-2355-1150 AD - Department of Neurology, Clinical Neuroscience Center, University Hospital Zurich, 8091 Zurich, Switzerland. FAU - Wegener, Susanne AU - Wegener S AD - Faculty of Medicine, University of Zurich, 8032 Zurich, Switzerland. AD - Department of Neurology, Clinical Neuroscience Center, University Hospital Zurich, 8091 Zurich, Switzerland. FAU - Luft, Andreas AU - Luft A AUID- ORCID: 0000-0001-9865-7382 AD - Faculty of Medicine, University of Zurich, 8032 Zurich, Switzerland. AD - Department of Neurology, Clinical Neuroscience Center, University Hospital Zurich, 8091 Zurich, Switzerland. AD - Cereneo Center for Neurology and Rehabilitation, 6354 Vitznau, Switzerland. FAU - Regli, Luca AU - Regli L AUID- ORCID: 0000-0003-4639-4474 AD - Department of Neurosurgery, Clinical Neuroscience Center, University Hospital Zurich, 8091 Zurich, Switzerland. AD - Faculty of Medicine, University of Zurich, 8032 Zurich, Switzerland. FAU - Esposito, Giuseppe AU - Esposito G AUID- ORCID: 0000-0001-8687-3109 AD - Department of Neurosurgery, Clinical Neuroscience Center, University Hospital Zurich, 8091 Zurich, Switzerland. AD - Faculty of Medicine, University of Zurich, 8032 Zurich, Switzerland. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702187 OTO - NOTNLM OT - carotid endarterectomy OT - internal carotid artery OT - intraoperative monitoring OT - transit-time flowmetry COIS- The authors declare no conflicts of interest. G.E. and L.R. serve as consultants for Aesculap B. Braun. G.E. is a member of the Board of Trustees of the Brain Disease Foundation. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:33 PHST- 2026/06/21 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - brainsci16101136 [pii] AID - brainsci-16-01136 [pii] AID - 10.3390/brainsci16101136 [doi] PST - epublish SO - Brain Sci. 2026 Oct 21;16(10):1136. doi: 10.3390/brainsci16101136. PMID- 42939382 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 20 TI - Endoscopic Anatomical Study of the Skull Base with Poor Pneumatization: A Quantitative Analysis Based on Cadaveric Specimens. LID - 10.3390/brainsci16101132 [doi] LID - 1132 AB - BACKGROUND: The endoscopic endonasal approach (EEA) has been widely applied in the surgical treatment of skull base tumors. However, poor pneumatization of the skull base-such as inadequate sphenoid sinus pneumatization and dense clival bone-can severely obscure key anatomical landmarks, significantly increasing surgical risks. Systematic quantitative studies on endoscopic anatomical landmarks under conditions of poor pneumatization remain limited to date. METHODS: Using 10 lightly embalmed adult cadaveric head specimens with red-blue silicone injection, we performed a stepwise dissection of the nasal cavity; sphenoid sinus; cavernous sinus (CS); upper, middle, and lower clivus; craniovertebral junction (CVJ); and lateral skull base regions via the expanded endoscopic EEA with a 0 degrees rigid endoscope. Particular emphasis was placed on observing variations in the landmarks within the sphenoid sinus under conditions of poor pneumatization, and morphometric measurements were obtained for key structures, including the clivus and its adjacent areas, occipital condyles (OC), hypoglossal canal (HC), petrous apex (PA), and vidian canal (VC). RESULTS: In poorly pneumatized specimens, the clival recess (CR) within the sphenoid sinus is often not observable, and the optic nerve prominence and the internal carotid artery (ICA) prominence are indistinct. Quantitative data showed that the total length of the clivus was 28.7 +/- 2.1 mm, with gradual widening from superior to inferior (upper clivus: 20.8 +/- 2.0 mm; middle clivus: 22.7 +/- 2.8 mm; lower clivus: 29.2 +/- 3.9 mm). The average drillable area of the entire clivus was 8.6 +/- 1.1 cm(2), and the PA could be safely drilled by approximately 45-48% (about 1.0 cm(3)) via the EEA. The length of the VC ranged from 17.1 to 17.8 mm, and its distance from the foramen rotundum (FR) was approximately 7.8-8.0 mm, which may serve as a useful anatomical reference for localizing the horizontal petrous segment of the ICA. CONCLUSIONS: In cases of poor pneumatization of the skull base, the osseous and vascular prominences are often obscured under EEA, necessitating coordinate-based localization using quantitative anatomical data. The present study provides segmental clival widths, three-dimensional measurements of the OC-HC complex, the VC-FR interval, and the safe drillable volume of the PA, thereby furnishing a quantitative reference for precise and safe surgery in the poorly pneumatized skull base. FAU - Wang, Benlin AU - Wang B AD - Department of Neurosurgery, Yantai Yuhuangding Hospital, Qingdao University, Yantai 264000, China. FAU - He, Qiaowei AU - He Q AD - Department of Neurosurgery, Yantai Yuhuangding Hospital, Qingdao University, Yantai 264000, China. FAU - Chi, Nan AU - Chi N AD - Department of Neurosurgery, Yantai Yuhuangding Hospital, Qingdao University, Yantai 264000, China. FAU - Zhang, Hongtao AU - Zhang H AUID- ORCID: 0009-0002-9952-6100 AD - Department of Neurosurgery, Yantai Yuhuangding Hospital, Qingdao University, Yantai 264000, China. FAU - Song, Xicheng AU - Song X AUID- ORCID: 0000-0002-9789-1318 AD - Department of Otorhinolaryngology, Head and Neck Surgery, Yantai Yuhuangding Hospital, Qingdao University, Yantai 264000, China. AD - Shandong Provincial Key Laboratory of Neuroimmune Interaction and Regulation, Yantai Yuhuangding Hospital, Yantai 264000, China. AD - Shandong Provincial Clinical Research Center for Otorhinolaryngologic Diseases, Yantai Yuhuangding Hospital, Yantai 264000, China. AD - Yantai Key Laboratory of Otorhinolaryngologic Diseases, Yantai Yuhuangding Hospital, Qingdao University, Yantai 264000, China. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702636 OTO - NOTNLM OT - endoscopic anatomy OT - poor pneumatization OT - quantitative analysis OT - skull base tumor COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:33 PHST- 2026/09/19 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - brainsci16101132 [pii] AID - brainsci-16-01132 [pii] AID - 10.3390/brainsci16101132 [doi] PST - epublish SO - Brain Sci. 2026 Oct 20;16(10):1132. doi: 10.3390/brainsci16101132. PMID- 42939376 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 17 TI - EOG Artifact Removal from EEG Using a Gated WGAN with Multi-Scale and Multi-Domain Feature Extraction. LID - 10.3390/brainsci16101124 [doi] LID - 1124 AB - Background: Electroencephalogram (EEG) signals provide important information for brain-computer interfaces (BCIs) and the diagnosis of neurological disorders but are susceptible to electrooculographic (EOG) artifacts. Traditional methods often rely on multichannel data or relatively long windows, limiting online applicability. Deep learning enables single-channel end-to-end artifact removal, but existing methods may not fully exploit features in the time and frequency domains and may unnecessarily modify relatively clean EEG. Methods: We developed a Wasserstein generative adversarial network (WGAN) integrating reconstruction loss, multi-domain and multi-scale feature extraction, and a gating unit. Reconstruction loss improved waveform reconstruction, yielding a reconstruction-loss-assisted artifact-removal WGAN (rarWGAN). Parallel branches with different kernel sizes extracted multi-scale features, while a multi-domain module combined temporal features with spectral features extracted using complex-valued convolutions. Integrating both modules into rarWGAN produced MDS-rarWGAN. Based on discriminator scores, gating routed inputs classified as contaminated to the generator and retained the others unchanged. Results: On EEGdenoiseNet, MDS-rarWGAN yielded relative root mean square errors (RRMSEs) of 0.189 +/- 0.028 and 0.183 +/- 0.027 in the time and frequency domains, respectively, with a correlation coefficient of 0.955 +/- 0.013. Compared with rarWGAN, RRMSEs decreased by 23.2% and 32.5%, respectively, and the correlation coefficient increased by 1.2%. The gating unit yielded false positive and false negative rates of 1.47% and 0.12%, respectively. For EEG containing artifacts, no statistically significant differences in time domain RRMSE were detected with and without gating (all p > 0.05). On BCI Competition IV 2a, reconstruction metrics measured agreement with a reference derived from independent component analysis (ICA), as artifact-free EEG ground truth was unavailable. Using the same EEGNet classifier, EEG processed by gated-MDS-rarWGAN achieved the highest mean motor imagery classification accuracy among the evaluated deep learning methods. Conclusions: The method suppresses EOG artifacts, reduces unnecessary processing of signals without evident artifacts, and supports motor imagery decoding, offering a feasible preprocessing approach. FAU - Xie, Songyun AU - Xie S AD - School of Electronics and Information, Northwestern Polytechnical University, Xi'an 710072, China. FAU - Liu, Xianghui AU - Liu X AD - School of Electronics and Information, Northwestern Polytechnical University, Xi'an 710072, China. FAU - Wang, Xiangming AU - Wang X AD - School of Electronics and Information, Northwestern Polytechnical University, Xi'an 710072, China. FAU - Xie, Xinzhou AU - Xie X AD - School of Electronics and Information, Northwestern Polytechnical University, Xi'an 710072, China. FAU - Tang, Hao AU - Tang H AD - School of Electronics and Information, Northwestern Polytechnical University, Xi'an 710072, China. LA - eng GR - 62220106007/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261017 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702287 OTO - NOTNLM OT - EEG OT - EOG OT - Wasserstein generative adversarial network OT - artifact removal OT - multi-scale feature extraction COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:33 PHST- 2026/08/14 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - brainsci16101124 [pii] AID - brainsci-16-01124 [pii] AID - 10.3390/brainsci16101124 [doi] PST - epublish SO - Brain Sci. 2026 Oct 17;16(10):1124. doi: 10.3390/brainsci16101124. PMID- 42939375 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 17 TI - Tractometric: A Diffusion Tractography Software Engine for Integration into a Neuronavigation System: Technical Development and Preliminary Clinical Feasibility. LID - 10.3390/brainsci16101123 [doi] LID - 1123 AB - BACKGROUND: Diffusion MRI tractography can support patient-specific neurosurgical planning, but implementation may be limited by workflow complexity, software requirements, and resource constraints. We describe Tractometric((R)), a diffusion tractography software engine developed for integration with the Surgimetric((R)) neuronavigation planning environment. METHODS: Tractometric((R)) was developed through a multidisciplinary, iterative co-design process involving neurosurgeons and neuroimaging specialists. The software uses a modular Model-View-Controller architecture implemented in Python (version 3.11) and supports multiple diffusion reconstruction models, deterministic and probabilistic tracking, patient-specific and atlas-derived regions of interest (ROIs), visualization, and interoperable export. The present study was designed as a technical development and preliminary clinical feasibility report. Technical workflow characteristics were described retrospectively across 30 cases, while preliminary clinical feasibility was explored in 8 patients undergoing tractography-assisted surgical planning. Intraoperative direct cortical and subcortical electrical stimulation was used as a qualitative clinical reference rather than as a formal diagnostic-accuracy test. RESULTS: Tractometric((R)) implemented a complete workflow from diffusion data loading and preprocessing to tract reconstruction, filtering, visualization, and export to Surgimetric((R)). The 30-case technical series illustrated variation in processing time and user interaction according to operator experience, reconstruction model, registration, ROI strategy, and manual cleaning. In 8 preliminary clinical cases, reconstructed motor and language pathways were qualitatively concordant with positive intraoperative stimulation findings. No formal sensitivity, specificity, spatial agreement, or test-retest reproducibility estimates were calculated. CONCLUSIONS: Tractometric((R)) provides an integrated technical workflow for diffusion tractography and neuronavigation planning and was feasible to implement in a preliminary clinical series. The current data support technical feasibility and workflow integration, but do not establish anatomical accuracy, reproducibility, clinical efficacy, or cost-effectiveness. Independent, prospective validation using standardized acquisitions, predefined tractography parameters, quantitative spatial metrics, and systematic intraoperative mapping is warranted. FAU - Mendez, William AU - Mendez W AD - Biomedical Engineering, Navimetric Latinoamerica S.A.S., Bogota 111211, Colombia. AD - Neurosurgery Research Department, Navimetric Latinoamerica S.A.S., Bogota 111211, Colombia. FAU - Bohorquez, Alejandra AU - Bohorquez A AD - Biomedical Engineering, Navimetric Latinoamerica S.A.S., Bogota 111211, Colombia. AD - Neurosurgery Research Department, Navimetric Latinoamerica S.A.S., Bogota 111211, Colombia. FAU - Bohorquez, Ximena AU - Bohorquez X AD - Biomedical Engineering, Navimetric Latinoamerica S.A.S., Bogota 111211, Colombia. AD - Neurosurgery Research Department, Navimetric Latinoamerica S.A.S., Bogota 111211, Colombia. FAU - Bohorquez, Oscar AU - Bohorquez O AD - Biomedical Engineering, Navimetric Latinoamerica S.A.S., Bogota 111211, Colombia. AD - Neurosurgery Research Department, Navimetric Latinoamerica S.A.S., Bogota 111211, Colombia. FAU - Shelton, William J AU - Shelton WJ AUID- ORCID: 0000-0002-9892-6690 AD - School of Medicine, Universidad de Los Andes, Bogota 111711, Colombia. FAU - Ordonez-Rubiano, Edgar G AU - Ordonez-Rubiano EG AUID- ORCID: 0000-0003-1179-5825 AD - Department of Neurosurgery, Hospital Universitario Fundacion Santa Fe de Bogota, Bogota 110111, Colombia. AD - Department of Neurosurgery, Fundacion Universitaria de Ciencias de La Salud-Universidad FUCS, Hospital de San Jose-Sociedad de Cirugia de Bogota, Bogota 110221, Colombia. LA - eng GR - Navimetric Latinoamerica S.A.S/ PT - Journal Article DEP - 20261017 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702410 OTO - NOTNLM OT - diffusion tensor imaging OT - low-to-middle-income countries OT - neuronavigation system OT - tractography COIS- William Mendez and Alejandra Bohorquez are employees of Navimetric Latinoamerica S.A.S. Edgar G. Ordonez-Rubiano serves as a clinical consultant for Navimetric Latinoamerica S.A.S. Oscar Bohorquez and Ximena Bohorquez are co-owners of Navimetric Latinoamerica S.A.S. Oscar and Ximena were involved in scientific oversight and final manuscript review. Because the software was developed within Navimetric and several authors participated in software development, implementation, testing, or clinical interpretation, the potential for developer and selection bias should be considered when interpreting the preliminary results. The present study did not include independent external validation. William J. Shelton declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/17 CRDT- 2026/10/27 10:33 PHST- 2026/08/25 00:00 [received] PHST- 2026/09/16 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - brainsci16101123 [pii] AID - brainsci-16-01123 [pii] AID - 10.3390/brainsci16101123 [doi] PST - epublish SO - Brain Sci. 2026 Oct 17;16(10):1123. doi: 10.3390/brainsci16101123. PMID- 42939373 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 16 TI - Neurosurgical Management of Refractory Trigeminal Neuralgia in Older Adults: A Structured Narrative Review. LID - 10.3390/brainsci16101121 [doi] LID - 1121 AB - BACKGROUND/OBJECTIVES: Trigeminal neuralgia (TN) disproportionately affects older adults, in whom medication intolerance, drug interactions, comorbidities, and limited physiological reserve may complicate management. This structured narrative review examines the evidence concerning the selection, effectiveness, durability, and complications of neurosurgical treatments for medication-refractory TN in older adults. METHODS: PubMed was searched from database inception through 1 July 2026 using terms related to TN, older age, and neurosurgical treatment. Eligible publications evaluated microvascular decompression (MVD), radiofrequency lesioning, percutaneous balloon compression (PBC), glycerol rhizotomy, or stereotactic radiosurgery (SRS). Titles and abstracts were screened using predefined eligibility criteria, followed by further assessment of potentially eligible publications and standardized data extraction. Studies reporting separately extractable outcomes for defined older populations were classified as direct evidence; mixed-age studies without age-stratified outcomes were treated as indirect evidence and used primarily for procedural and comparative context. RESULTS: The principal search identified 1324 unique records, and 22 publications were included: six provided direct older-adult evidence and 16 provided indirect contextual evidence. Direct evidence was largely limited to MVD and PBC. In selected older adults, MVD was associated with favorable and potentially durable pain outcomes, whereas PBC generally provided rapid initial pain relief. However, differences in outcome definitions, assessment times, follow-up, and patient selection precluded direct comparison. No included study provided separately extractable outcomes for adults aged >/=65 years undergoing radiofrequency lesioning, glycerol rhizotomy, or SRS. GRADE certainty was low or very low for most procedure-specific and comparative conclusions. CONCLUSIONS: Favorable observational outcomes in selected older patients suggest that chronological age alone should not automatically exclude an otherwise appropriate patient from consideration for MVD. Treatment should be individualized according to TN subtype, physiological reserve, comorbidity and anesthetic risk, imaging findings, pain urgency, previous interventions, baseline facial sensation, and patient preferences. FAU - Najjar, Ahmed AU - Najjar A AD - Department of General and Specialized Surgery, College of Medicine, Taibah University, Almadinah 42353, Saudi Arabia. AD - Department of Surgery, King Faisal Specialist Hospital and Research Center, Almadinah 42353, Saudi Arabia. FAU - Elhassan, Yasir Hassan AU - Elhassan YH AUID- ORCID: 0000-0003-0196-5413 AD - Department of Basic Medical Sciences, College of Medicine, Taibah University, Almadinah 42353, Saudi Arabia. LA - eng PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702137 OTO - NOTNLM OT - balloon compression OT - frailty OT - glycerol rhizotomy OT - microvascular decompression OT - older adults OT - radiofrequency rhizotomy OT - stereotactic radiosurgery OT - trigeminal neuralgia COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:33 PHST- 2026/08/16 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - brainsci16101121 [pii] AID - brainsci-16-01121 [pii] AID - 10.3390/brainsci16101121 [doi] PST - epublish SO - Brain Sci. 2026 Oct 16;16(10):1121. doi: 10.3390/brainsci16101121. PMID- 42939369 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 16 TI - Dynamic Evolution of Quantitative Electroencephalography During Graded Intracranial Hypertension and Screening of Candidate Early-Warning Features in a Rabbit Model. LID - 10.3390/brainsci16101116 [doi] LID - 1116 AB - Background and Objectives: Elevated intracranial pressure (ICP) is common to many encephalopathies, but the evolution of quantitative electroencephalography (qEEG) indices along a pressure gradient-and their relative suitability for early warning-have not been systematically compared. In this exploratory study we established a stepwise, steady-state rabbit model of graded intracranial hypertension and screened candidate early-warning features against prespecified criteria. Materials and Methods: Eight male New Zealand rabbits were studied; two were excluded-one for circulatory failure during catheterization and one for refractory status epilepticus that reached a humane endpoint-leaving six that completed the protocol. Repeated small-volume inflation of a left parenchymal water balloon was used to define five windows: baseline (BL; ICP < 10 mmHg), 15-20, 25-30 and 35-40 mmHg, and an end stage (END). Eight-channel EEG was recorded synchronously, and delta, theta, alpha and beta power, together with the amplitude-integrated EEG (aEEG) upper margin, lower margin and bandwidth, were computed. Seven candidate features and three criteria (evolution timing, individual consistency and strength of association with ICP) were fixed before analysis; selection stability was assessed by leave-one-rabbit-out (LORO) cross-validation. Results: ICP rose in a controlled manner from 5.43 +/- 1.09 to 35.93 +/- 1.48 mmHg (Kendall W = 0.96), without systematic changes in respiratory mechanics. As ICP increased, absolute theta and alpha power declined progressively (rho = -0.77 and -0.53), whereas absolute delta power did not increase and its rising relative share merely reflected redistribution after the loss of faster activity. The three aEEG components changed asynchronously: the lower margin shifted downward early and persistently (rho = -0.87 with ICP; W = 0.86), whereas the upper margin changed only terminally and bandwidth showed no consistent direction. Concurrently, MAP rose through the Cushing response, with heart-rate slowing, but the rise was insufficient and CPP fell from 64.78 to 41.24 mmHg, approaching the rabbit lower limit of autoregulation (~50 mmHg CPP) only in the later windows. Because ICP and CPP were tightly coupled within animals, their independent effects on the EEG could not be separated. In leave-one-rabbit-out cross-validation, the lower margin was selected in five of six folds, with a median out-of-sample rho of -1.00 (a discrete value based on four windows per animal). Conclusions: In this exploratory study in a small cohort (n = 6), qEEG indices evolved asynchronously along the ICP gradient; the aEEG lower margin appeared earliest and remained relatively stable across leave-one-rabbit-out folds, making it a candidate early-warning feature worthy of priority validation. However, because rising ICP and falling CPP were tightly coupled and could not be separated in this model, its early decline cannot be uniquely attributed to either ICP elevation or hypoperfusion. Its alarm threshold and early-warning value still require confirmation in larger independent cohorts with simultaneous CPP and cerebral blood flow monitoring. FAU - Zhao, Jianxiang AU - Zhao J AUID- ORCID: 0009-0004-2170-061X AD - Shengli Clinical Medical College of Fujian Medical University, Fuzhou 350001, China. AD - Department of Critical Care Medicine, Fujian Provincial Hospital, Fuzhou 350001, China. AD - Fuzhou University Affiliated Provincial Hospital, Fuzhou 350001, China. AD - Fujian Provincial Key Laboratory of Emergency Medicine, Fuzhou 350001, China. FAU - Zhang, Yang AU - Zhang Y AD - Shengli Clinical Medical College of Fujian Medical University, Fuzhou 350001, China. AD - Fuzhou University Affiliated Provincial Hospital, Fuzhou 350001, China. AD - Department of Neurosurgery, Fujian Provincial Hospital, Fuzhou 350001, China. FAU - Wang, Kaiyu AU - Wang K AD - Shengli Clinical Medical College of Fujian Medical University, Fuzhou 350001, China. AD - Fuzhou University Affiliated Provincial Hospital, Fuzhou 350001, China. AD - Department of Neurosurgery, Fujian Provincial Hospital, Fuzhou 350001, China. FAU - Chen, Feng AU - Chen F AUID- ORCID: 0009-0008-7216-9282 AD - Shengli Clinical Medical College of Fujian Medical University, Fuzhou 350001, China. AD - Fuzhou University Affiliated Provincial Hospital, Fuzhou 350001, China. AD - Fujian Provincial Key Laboratory of Emergency Medicine, Fuzhou 350001, China. AD - Department of Emergency, Fujian Provincial Hospital, Fuzhou 350001, China. FAU - Chen, Han AU - Chen H AD - Shengli Clinical Medical College of Fujian Medical University, Fuzhou 350001, China. AD - Department of Critical Care Medicine, Fujian Provincial Hospital, Fuzhou 350001, China. AD - Fuzhou University Affiliated Provincial Hospital, Fuzhou 350001, China. LA - eng GR - 2021J01368/Fujian Provincial Department of Science and Technology/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702719 OTO - NOTNLM OT - EEG spectral band power OT - amplitude-integrated electroencephalography OT - candidate early-warning feature OT - intracranial pressure OT - quantitative electroencephalography OT - rabbit COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/16 CRDT- 2026/10/27 10:33 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - brainsci16101116 [pii] AID - brainsci-16-01116 [pii] AID - 10.3390/brainsci16101116 [doi] PST - epublish SO - Brain Sci. 2026 Oct 16;16(10):1116. doi: 10.3390/brainsci16101116. PMID- 42939366 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 15 TI - Intentional Versus Incidental Encoding of Emotional and Navigational Memory: Preliminary Data in Healthy Young Adults. LID - 10.3390/brainsci16101113 [doi] LID - 1113 AB - BACKGROUND/OBJECTIVES: episodic memory refers to the ability to place events within a specific spatial and temporal context, making its association with navigation unsurprising. Incidental episodic memory involves encoding and retrieving information without explicit instruction, a common process in everyday life whose sensitivity to emotional valence warrants further investigation. METHODS: sixty-three college students (mean age = 25 years; 28 females) completed two versions of the same task, differing only in the instructions given: one directed attention to emotionally charged images encountered along a route through a city environment; the other directed attention to the route itself. RESULTS: Mann-Whitney tests on uncontaminated first-session conditions and linear mixed models all indicated an advantage of intentional over incidental encoding for the spatial localization of emotional images. The advantage was valence-selective, present for negative and neutral stimuli but absent for positive ones. Signal detection analyses indicated that intentional instructions selectively increased sensitivity (d'). The response criterion also shifted, but in opposite directions for negative and neutral stimuli, so the sensitivity gain cannot be attributed to a uniform change in response bias. The effect was domain-specific: sensitivity for the spatial localization of emotional images increased, whereas sensitivity to navigational route did not improve. CONCLUSIONS: positive material thus appears to be encoded spontaneously, with potential implications for rehabilitation and clinical assessment. FAU - Di Piero, Andrea AU - Di Piero A AD - Department of Psychology, Sapienza University of Rome, 00185 Rome, Italy. FAU - Bonavita, Alessia AU - Bonavita A AUID- ORCID: 0000-0001-7793-263X AD - Faculty of Law, Giustino Fortunato University, 82100 Benevento, Italy. FAU - Piccardi, Laura AU - Piccardi L AUID- ORCID: 0000-0002-1322-5538 AD - Department of Psychology, Sapienza University of Rome, 00185 Rome, Italy. AD - IRCCS San Raffaele Cassino Hospital, 03043 Cassino, Italy. FAU - Casagrande, Maria AU - Casagrande M AUID- ORCID: 0000-0002-4430-3367 AD - Department of Dynamic and Clinical Psychology, Sapienza University of Rome, 00185 Rome, Italy. FAU - Guariglia, Cecilia AU - Guariglia C AD - Department of Psychology, Sapienza University of Rome, 00185 Rome, Italy. AD - Cognitive and Motor Rehabilitation and Neuroimaging Unit, IRCCS Fondazione Santa Lucia, 00179 Rome, Italy. AD - Department of Medicine and Health Sciences, University of Molise, 86100 Campobasso, Italy. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702390 OTO - NOTNLM OT - emotional landmarks OT - episodic memory OT - familiarity OT - incidental memory OT - landmark-based navigation OT - navigation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:33 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - brainsci16101113 [pii] AID - brainsci-16-01113 [pii] AID - 10.3390/brainsci16101113 [doi] PST - epublish SO - Brain Sci. 2026 Oct 15;16(10):1113. doi: 10.3390/brainsci16101113. PMID- 42939365 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 15 TI - Accuracy of Video Optical See-Through Augmented Reality Surgical System (VOSTARS) to Guide Craniotomy: A Phantom and a Human Cadaver Study. LID - 10.3390/brainsci16101112 [doi] LID - 1112 AB - BACKGROUND: Augmented reality (AR) may support neuronavigation by displaying patient-specific anatomical information and surgical plans directly within the surgeon's view of the operative field. Reliable registration between virtual content and patient anatomy remains a critical requirement. Patient-specific templates offer a non-invasive registration strategy, but positioning errors at the facial support may propagate to the surgical target. METHODS: We performed a preclinical feasibility evaluation of the Video and Optical See-Through Augmented Reality Surgical System (VOSTARS), a head-mounted AR platform for surgical navigation, using patient-specific template-based registration. Six craniotomies were planned using CT datasets from two fresh-frozen human cadaver heads and two corresponding cranial phantoms were generated from the same datasets. Visualization accuracy, reported as target visualization error (TVE(3)D), and trajectory-tracing performance were first assessed on the phantoms. The complete AR-guided planning-to-execution workflow was subsequently evaluated on the corresponding cadaveric specimens by assessing containment of the AR-traced craniotomy trajectories within patient-specific inspection windows corresponding to a tolerance of +/-1.5 mm. RESULTS: In the phantom experiments, the mean TVE(3)D calculated across five of the six planned craniotomies was 1.64 mm, with individual mean values ranging from 1.33 to 1.91 mm. Craniotomy 1 had a mean TVE(3)D of 6.92 mm; the higher error was attributed to incorrect phantom assembly. Four of the six craniotomy trajectories traced on the phantoms under AR guidance showed complete overlap with the corresponding virtual trajectories. For the other two, trajectory overlap was 62.5% and 67.0%, with a mean deviation from the planned trajectory of 0.79 mm in both cases. In the cadaveric experiments, the complete AR-guided planning-to-execution workflow was completed for all six planned craniotomies. Two of the six AR-traced craniotomy trajectories were completely contained within the inspection windows corresponding to the predefined +/-1.5-mm tolerance. Of the remaining four trajectories, two were partially contained, one was minimally contained and one was not contained within the inspection window. CONCLUSIONS: This study supports the technical feasibility of a patient-specific AR workflow for craniotomy guidance across anatomically different cranial approaches. In this exploratory preclinical study, VOSTARS enabled the translation of patient-specific preoperative planning directly to the operative field and provided AR guidance during the initial stages of cranial surgery. The cadaveric findings also highlight template fit and positioning as important determinants of workflow performance. Further quantitative and comparative validation is required before clinical implementation. FAU - Montemurro, Nicola AU - Montemurro N AUID- ORCID: 0000-0002-3686-8907 AD - Department of Neurosurgery, Azienda Ospedaliero Universitaria Pisana (AOUP), 56100 Pisa, Italy. AD - EndoCAS Interdipartimental Center, University of Pisa, 56100 Pisa, Italy. FAU - Carbone, Marina AU - Carbone M AUID- ORCID: 0000-0002-1004-6964 AD - EndoCAS Interdipartimental Center, University of Pisa, 56100 Pisa, Italy. AD - Department of Information Engineering, University of Pisa, 56100 Pisa, Italy. FAU - Viglialoro, Rosanna Maria AU - Viglialoro RM AD - EndoCAS Interdipartimental Center, University of Pisa, 56100 Pisa, Italy. FAU - Simi, Francesco AU - Simi F AD - EndoCAS Interdipartimental Center, University of Pisa, 56100 Pisa, Italy. FAU - Zari, Giulia AU - Zari G AUID- ORCID: 0009-0009-8913-5107 AD - EndoCAS Interdipartimental Center, University of Pisa, 56100 Pisa, Italy. FAU - Condino, Sara AU - Condino S AUID- ORCID: 0000-0001-5235-0414 AD - EndoCAS Interdipartimental Center, University of Pisa, 56100 Pisa, Italy. AD - Department of Information Engineering, University of Pisa, 56100 Pisa, Italy. FAU - Acerbi, Francesco AU - Acerbi F AD - Department of Neurosurgery, Azienda Ospedaliero Universitaria Pisana (AOUP), 56100 Pisa, Italy. AD - Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56100 Pisa, Italy. LA - eng GR - GR-2021-12373198/Ministry of Health/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702260 OTO - NOTNLM OT - augmented reality OT - cadaver study OT - craniotomy OT - head-mounted display OT - image-guided neuronavigation OT - neuronavigation OT - neurosurgery OT - preoperative planning OT - surgical planning COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/15 CRDT- 2026/10/27 10:33 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - brainsci16101112 [pii] AID - brainsci-16-01112 [pii] AID - 10.3390/brainsci16101112 [doi] PST - epublish SO - Brain Sci. 2026 Oct 15;16(10):1112. doi: 10.3390/brainsci16101112. PMID- 42939362 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 14 TI - Analysis of Selected Cognitive Abilities and Temperamental Traits Among Young Adults Playing Video Games. LID - 10.3390/brainsci16101109 [doi] LID - 1109 AB - Objectives: The aim of this study was to analyse cognitive functioning and temperamental traits among young adults differing in their level of engagement in video gaming. Methods: The analysis included 67 male students from the Jozef Pilsudski University of Physical Education in Warsaw, Branch Campus in Biala Podlaska. Based on average daily gaming time, participants were classified as frequent players (n = 22; mean age 22.45 +/- 2.40 years; 6.29 +/- 6.07 h/day) or casual players (n = 45; mean age 21.60 +/- 1.96 years; 0.94 +/- 0.95 h/day). Cognitive functioning was assessed using the Reaction Test (RT), Signal Test (ST), and Determination Test (DT) from the Vienna Test System. Temperament was measured with the Formal Characteristics of Behaviour-Temperament Inventory. Results: Frequent players showed a trend toward shorter reaction time in RT/S3 (U = 356; p = 0.064). In the Signal Test, they demonstrated significantly fewer omitted reactions, more correct responses, higher focused attention, and better total results (all p < 0.01). Regarding temperament, frequent players scored significantly lower on briskness (p = 0.034) and rhythmicity (p = 0.021) than casual players. Conclusions: The present exploratory study suggests a nuanced relationship between media engagement, specific cognitive performance, and temperamental traits. Frequent players demonstrated a robust advantage in parameters of sustained attention, alongside a marginal descriptive trend toward faster psychomotor responses compared to casual players. Regarding temperament, frequent players were characterised by significantly lower self-reported briskness and rhythmicity, while no statistically significant between-group differences were detected for endurance, activity, or emotional reactivity. Crucially, because our data are cross-sectional and the frequent players group comprised exclusively esports students, these differences cannot be interpreted as definitive causal effects or direct consequences of gaming intensity alone. FAU - Krzepota, Justyna AU - Krzepota J AUID- ORCID: 0000-0002-0110-8676 AD - Institute of Physical Culture Sciences, University of Szczecin, 70-453 Szczecin, Poland. FAU - Wrzesniewski, Krzysztof AU - Wrzesniewski K AUID- ORCID: 0000-0003-3443-5638 AD - Department of Psychology, Faculty of Physical Education and Sport, University of Physical Culture in Krakow, 31-571 Krakow, Poland. FAU - Basiaga-Pasternak, Joanna AU - Basiaga-Pasternak J AUID- ORCID: 0000-0002-6875-4875 AD - Department of Psychology, Faculty of Physical Education and Sport, University of Physical Culture in Krakow, 31-571 Krakow, Poland. FAU - Ciesluk, Marek AU - Ciesluk M AUID- ORCID: 0009-0006-0649-7363 AD - Department of Social and Developmental Psychology, Institute of Psychology, University of Szczecin, 70-453 Szczecin, Poland. FAU - Luc, Aneta AU - Luc A AD - Regional Research and Development Center, Faculty of Physical Education and Sport in Biala Podlaska, Jozef Pilsudski University of Physical Education in Warsaw, 21-500 Biala Podlaska, Poland. FAU - Sacewicz, Tomasz AU - Sacewicz T AUID- ORCID: 0000-0002-4417-9220 AD - Department of Biomechanics and Anatomy, Faculty of Physical Education and Health in Biala Podlaska, Jozef Pilsudski University of Physical Education in Warsaw, 21-500 Biala Podlaska, Poland. FAU - Sadowska, Dorota AU - Sadowska D AUID- ORCID: 0000-0002-9823-7991 AD - Institute of Sport-National Research Institute, 01-982 Warsaw, Poland. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702366 OTO - NOTNLM OT - Vienna Test System OT - cognitive abilities OT - gamers OT - temperamental traits OT - the Formal Characteristics of Behaviour-Temperament Inventory OT - video games COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:33 PHST- 2026/08/13 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - brainsci16101109 [pii] AID - brainsci-16-01109 [pii] AID - 10.3390/brainsci16101109 [doi] PST - epublish SO - Brain Sci. 2026 Oct 14;16(10):1109. doi: 10.3390/brainsci16101109. PMID- 42939359 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 14 TI - A Proprioception-Centered Hypothesis Linking Sensorimotor Function, Respiration, and Sleep Across the Day-Night Cycle in Developmental Dyslexia. LID - 10.3390/brainsci16101106 [doi] LID - 1106 AB - Objectives: Developmental dyslexia is a heterogeneous neurodevelopmental condition in which motor, perceptual, attentional, respiratory, and sleep-related differences have been reported alongside reading difficulties. This Perspective proposes a proprioception-centered sensorimotor coherence framework to consider potential interactions among these domains across the day-night cycle. Methods: This hypothesis-generating narrative Perspective traces the progressive development of the proposed framework from clinical observations to experimental investigations and subsequent refinement of a clinical examination based on functional responses to sensory modulation. No systematic review or new cohort analysis was undertaken. The examination explores three principal domains-tonic-motor regulation, spatial localization, and multisensory integration-together with respiratory and sleep-related factors. Sensorimotor modulation includes ocular, oral, plantar, respiratory, manual, and ergonomic approaches selected according to their observed functional effects across domains. A clinical case illustrates the application of this approach. Results: Experimental studies conducted over nearly two decades in our INSERM laboratory objectively investigated postural regulation, proprioceptive acuity, spatial localization, multisensory processing, and action representation in developmental dyslexia. More recent exploratory clinical studies extended the investigation to respiration and sleep. Taken together, these observations suggest that proprioception-centered sensorimotor coherence may represent one functional level through which motor, perceptual, respiratory, attentional, and learning-related processes interact across the day-night cycle as learning develops and continues. Conclusions: The proposed framework is a working hypothesis rather than an established causal model or a validated treatment for developmental dyslexia. It provides a complementary embodied perspective and identifies clinically observable and experimentally testable relationships that warrant further investigation, including through controlled studies examining the contributions of individual sensory and functional components. FAU - Quercia, Patrick AU - Quercia P AUID- ORCID: 0000-0001-8151-7076 AD - CAPS UMR 1093, INSERM, Universite Bourgogne Europe, F-21000 Dijon, France. FAU - Morin, Steeve AU - Morin S AD - Cabinet de Podolologie, F-64120 Amendeuix-Oneix, France. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702614 OTO - NOTNLM OT - child sleep OT - developmental dyslexia OT - embodied cognition OT - motor control OT - multisensory integration OT - neuroplasticity OT - proprioception OT - sensorimotor coherence OT - sleep-disordered breathing OT - sleep-wake cycle COIS- Dr. Patrick Quercia has a long-standing clinical practice involving the assessment and management of children with developmental dyslexia, including the clinical observation of sensorimotor and proprioceptive features. He also holds an unpaid associate research position within INSERM. Steeve Morin sees patients as part of his ordinary professional clinical practice. The research activities underlying the present manuscript were conducted without personal remuneration to the authors. Neither author has any ownership, licensing, royalty, consultancy, or other proprietary financial interest in the techniques, devices, or materials discussed in this manuscript. Relevant institutional affiliations, research support, and professional associations are disclosed separately. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:33 PHST- 2026/07/22 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - brainsci16101106 [pii] AID - brainsci-16-01106 [pii] AID - 10.3390/brainsci16101106 [doi] PST - epublish SO - Brain Sci. 2026 Oct 14;16(10):1106. doi: 10.3390/brainsci16101106. PMID- 42939352 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 12 TI - D-Dimer as a Novel Factor for Recurrent Chronic Subdural Hematoma: Development and Assessment of a New Predictive Nomogram. LID - 10.3390/brainsci16101099 [doi] LID - 1099 AB - BACKGROUND: Chronic subdural hematoma (CSDH) is the most common bleeding disorder in the nervous system and has a high recurrence rate, posing a serious threat to the lives of elderly individuals. Early identification and adjustment of the treatment regimen in the perioperative period is crucial. The aim of this study was to create a perioperative predictive nomogram for the early prediction of recurrent CSDH (R-CSDH). METHODS: A predictive model was developed including 632 patients with CSDH hospitalized between 1 January 2021 and 31 December 2025 from Wuhan University People's Hospital. The 206 patients from the First People's Hospital of Xiantao City who were hospitalized between 1 January 2022 and 31 December 2025 formed an external validation cohort. Minimum absolute shrinkage was used to optimize feature selection, and logistic regression analysis was applied to further select features and construct a risk prediction model for recurrent CSDH. The calibration, discrimination, and clinical utility of the predictive models were assessed using calibration plots, the C-index, the area under the curve (AUC), and decision curve analysis. External validation was performed by an external validation cohort. FINDINGS: Age, CT hematoma size, hematoma location, atrophy, density, surgical procedure and D-dimer were the predictors for constructing the predictive nomogram, and the results showed that a logistic regression model based on the above seven factors had good calibration and discriminative ability (C-index: 0.925; 95% confidence interval: 0.893-0.957). The AUC of this predictive model was 0.92487. The calibration curve of the nomogram was close to the ideal diagonal. In addition, the decision curve analysis showed that the net benefit of the model was significantly better. External validation demonstrated the reliability of the predictive nomogram. INTERPRETATION: D-dimer was found to be a novel factor for recurrent chronic subdural hematoma. Our study provides clinicians with an accurate tool for identification and early intervention and therapy to reduce the incidence of R-CSDH. FAU - Wei, Jiajun AU - Wei J AD - Department of Neurosurgery, Renmin Hospital of Wuhan University, No. 238, Jiefang Road, Wuchang District, Wuhan 430060, China. FAU - Li, Zhiyang AU - Li Z AUID- ORCID: 0009-0009-6034-4095 AD - Department of Neurosurgery, Renmin Hospital of Wuhan University, No. 238, Jiefang Road, Wuchang District, Wuhan 430060, China. FAU - Wang, Wenju AU - Wang W AD - Department of Neurosurgery, Xiantao First People's Hospital Affiliated to Changjiang University, Xiantao 433000, China. FAU - Cai, Qiang AU - Cai Q AUID- ORCID: 0009-0002-5676-6307 AD - Department of Neurosurgery, Renmin Hospital of Wuhan University, No. 238, Jiefang Road, Wuchang District, Wuhan 430060, China. LA - eng PT - Journal Article DEP - 20261012 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702543 OTO - NOTNLM OT - D-dimer OT - chronic subdural hematoma OT - postoperative OT - predictive nomogram OT - recurrence COIS- The authors declare no competing interests. The authors claim that none of the material in the paper has been published or is under consideration for publication elsewhere, and no potential conflicts of interest were disclosed. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:33 PHST- 2026/08/25 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - brainsci16101099 [pii] AID - brainsci-16-01099 [pii] AID - 10.3390/brainsci16101099 [doi] PST - epublish SO - Brain Sci. 2026 Oct 12;16(10):1099. doi: 10.3390/brainsci16101099. PMID- 42939347 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 10 TI - Head-Mounted Augmented Reality in the Planning of Cerebral Cavernous Malformation Resections: Demonstrating Feasibility Through an Initial Case Series. LID - 10.3390/brainsci16101094 [doi] LID - 1094 AB - OBJECTIVES: Lesion localization, associated vascular structures, and corticotomy selection pose key challenges when planning cerebral cavernous malformation (CCM) resections in eloquent areas. Head-mounted augmented reality (HMAR) may improve visualization without the gaze disruptions imposed by conventional neuronavigation. This study investigated the preoperative feasibility of HMAR in surgical plan modifications and educational value. METHODS: We performed a single-center retrospective review of all CCM patients with HMAR-assisted preoperative planning from 1 September, 2022, to 31 August, 2024. Three-dimensional reconstructions of patients' magnetic resonance imaging were viewed preoperatively using HMAR. Registration accuracy was confirmed against conventional neuronavigation. Surgical plan modifications entailed alterations of the operative goal, trajectory, or steps. Educational value of HMAR was appraised by trainees as "not helpful" when only confirming existing plans, "helpful" when facilitating a greater understanding of the lesion, and "very helpful" when spurring additional positioning or plan modifications. RESULTS: Of 19 patients with CCM resections, 14 patients with HMAR-assisted preoperative planning were included. The average age was 38.6 +/- 14.5 years, with 6 males and 8 females. Three of 14 (21%) surgical approaches were modified following HMAR use: two to minimize cortical disruption and one to avoid injuring an associated developmental venous anomaly. Registration was accurate in all cases. Between registration and utilization, HMAR added an average of 2.7 +/- 1.0 min in the operating room. The mean appraisal of the educational value of HMAR was "helpful." CONCLUSIONS: Preoperative planning with HMAR prompted surgical plan modifications and often improved trainees' understanding without introducing significant workflow disruptions. Three-dimensional visualization offered dynamic spatial details beyond traditional two-dimensional multiplanar MRI. FAU - Jouppi, Luke L AU - Jouppi LL AUID- ORCID: 0009-0002-9651-2651 AD - Seattle Science Foundation, Seattle, WA 98122, USA. AD - Spectrum Research Consulting, Tacoma, WA 98405, USA. FAU - Pierre, Clifford A AU - Pierre CA AUID- ORCID: 0000-0001-9074-2435 AD - Seattle Science Foundation, Seattle, WA 98122, USA. AD - Department of Neurosurgery, Swedish Neuroscience Institute, Seattle, WA 98122, USA. FAU - Costa, Matias Luis AU - Costa ML AD - Seattle Science Foundation, Seattle, WA 98122, USA. AD - Department of Neurosurgery, Swedish Neuroscience Institute, Seattle, WA 98122, USA. AD - Department of Neurosurgery, The University of Texas Medical Branch at Galveston, Galveston, TX 77555, USA. FAU - Takroni, Radwan I AU - Takroni RI AD - Seattle Science Foundation, Seattle, WA 98122, USA. AD - Department of Neurosurgery, Aseer Central Hospital, Abha 62523, Saudi Arabia. FAU - Caffes, Nicholas AU - Caffes N AD - Seattle Science Foundation, Seattle, WA 98122, USA. AD - Wellspan Neurosurgery, Wellspan York Hospital, York, PA 17403, USA. FAU - Capek, Stepan AU - Capek S AD - Seattle Science Foundation, Seattle, WA 98122, USA. AD - Department of Neurosciences, Charleston Area Medical Center, Charleston, WV 25301, USA. FAU - Gustin, Aaron J AU - Gustin AJ AD - Seattle Science Foundation, Seattle, WA 98122, USA. AD - St Vincent Regional Hospital Brain and Spine, Billings, MT 59101, USA. FAU - Aljoghaiman, Majid AU - Aljoghaiman M AUID- ORCID: 0000-0002-3381-3564 AD - Seattle Science Foundation, Seattle, WA 98122, USA. AD - Surgery Department, College of Medicine, King Faisal University, Al-Hofuf 36362, Saudi Arabia. FAU - Tymchak, Zane A AU - Tymchak ZA AD - Seattle Science Foundation, Seattle, WA 98122, USA. AD - Department of Neurosurgery, Swedish Neuroscience Institute, Seattle, WA 98122, USA. FAU - Patel, Akshal S AU - Patel AS AD - Seattle Science Foundation, Seattle, WA 98122, USA. AD - Department of Neurosurgery, Swedish Neuroscience Institute, Seattle, WA 98122, USA. FAU - Monteith, Stephen J AU - Monteith SJ AUID- ORCID: 0009-0005-4023-771X AD - Seattle Science Foundation, Seattle, WA 98122, USA. AD - Department of Neurosurgery, Swedish Neuroscience Institute, Seattle, WA 98122, USA. LA - eng PT - Journal Article DEP - 20261010 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702605 OTO - NOTNLM OT - augmented reality OT - case series OT - cavernoma OT - cavernous malformation OT - head-mounted augmented reality OT - microsurgery COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:33 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - brainsci16101094 [pii] AID - brainsci-16-01094 [pii] AID - 10.3390/brainsci16101094 [doi] PST - epublish SO - Brain Sci. 2026 Oct 10;16(10):1094. doi: 10.3390/brainsci16101094. PMID- 42939341 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 9 TI - Fronto-Parietal Brain Metabolites Following Traumatic Brain Injury-A Proton MR Spectroscopy Study. LID - 10.3390/brainsci16101088 [doi] LID - 1088 AB - Background: Conventional structural CT and MRI may be normal after mild traumatic brain injury (mTBI), while magnetic resonance spectroscopic imaging ((1)H-MRSI) can provide regional measures of brain metabolites. We examined supracallosal fronto-parietal metabolite ratios in active-duty service members with military-related TBI and non-TBI military controls, including comparisons by injury mechanism and exploratory clinical associations. Methods: The study used a retrospective cross-sectional cohort. Participants underwent 3T 2D multivoxel (1)H-MRSI covering a 6 x 8 x 1 cm(3) supracallosal fronto-parietal PRESS/CSI volume of interest. Regional N-acetylaspartate (NAA), choline-containing compounds (Cho), and creatine/phosphocreatine (Cr) measures were processed with SIVIC and summarized as NAA/Cr and Cho/Cr ratios. Linear mixed-effects models were adjusted for age and sex, with Bonferroni correction for regional group comparisons. Male-only analyses were performed as a sensitivity analysis. Associations with clinical and neurocognitive measures were exploratory. Results: The final analytic cohort reported in the manuscript comprised 741 service members with TBI (34.3 +/- 8.1 years old; M/F = 711/30; mean 327.4 +/- 226.8 days post-injury) and 75 non-TBI controls (M/F = 53/22; mean age: 33.1 +/- 10.0 years). TBI participants showed lower Cho/Cr area ratios in multiple fronto-parietal regions, including right precuneus (estimate = -0.0781, SE = 0.0176, t = -4.43, pBonf < 0.0001, Cohen's d = -0.36, 95% CI [-0.1127, -0.0435]), left superior frontal gyrus (estimate = -0.0715, SE = 0.0163, t = -4.38, pBonf < 0.0001, Cohen's d = -0.36, 95% CI [-0.1035, -0.0394], and bilateral fronto-parietal WM (right: estimate = -0.0741, SE = 0.0169, t = -4.39, pBonf < 0.0001, Cohen's d = -0.35, 95% CI [-0.1072, -0.0410]; left: estimate = -0.0656, SE = 0.0161, t = -4.08, Cohen's d = -0.33, pBonf = 0.0011, 95% CI [-0.0971, -0.0340]). Injury mechanism stratification revealed a widespread reduction in Cho/Cr area in individuals with blast-related TBI relative to unexposed non-TBI controls over the left superior frontal gyrus (estimate = -0.0941, SE = 0.0176, t = -5.35, pBonf < 0.0001, Cohen's d = -0.44, 95% CI [-0.1287, -0.0596]), right precuneus (estimate = -0.0900, SE = 0.0193, t = -4.66, Cohen's d = -0.38, pBonf = 0.0010, 95% CI [-0.1279, -0.0521]), and right fronto-parietal WM (estimate = -0.0895, SE = 0.0182, t = -4.91, Cohen's d = -0.39, pBonf < 0.0001, 95% CI [-0.1253, -0.0537]). Twelve regional differences remained significant in the primary analysis, and the principal pattern was substantially preserved in the male-only sensitivity analysis. Blast-related and multiple-blast comparisons also showed regional Cho/Cr differences; individuals with multiple blast exposures mainly drove Cho/Cr reduction. Exploratory clinical analyses found inverse associations between Cho/Cr and Post-Traumatic Stress Checklist-Civilian version (PCL-C) and Neurobehavioral Symptom Inventory (NSI) scores, and positive associations between selected NAA/Cr measures and cognitive performance. Specifically, higher NAA/Cr area over the left caudal anterior cingulate correlates positively with verbal fluency performance (r = 0.358, p < 0.0001). Higher NAA/Cr peak heights in the left caudal middle frontal cortex correlated with better response inhibition accuracy (r = 0.278, p = 0.0003). Concurrently, lower Cho/Cr values over default mode network hubs-specifically the left caudal anterior cingulate gyrus (estimate = -0.00144, t = -4.02, uncorrected p < 0.0001, 95% CI [-0.00215, -0.00074]), left posterior cingulate gyrus (estimate = -0.00115, t = -3.76, uncorrected p = 0.00019, 95% CI [-0.00175, -0.00055], and the left precuneus gyrus (estimate = -0.00120, t = -3.62, p = 0.00033, 95% CI [-0.00186, -0.00055])-associated significantly with greater self-reported post-traumatic stress severity on the PCL-C. In addition, Cho/Cr in the left fronto-parietal WM was inversely associated with PCL-C (estimate = -0.00115, t = -3.87, p = 0.00012, 95% CI [-0.00174, -0.00057]) and NSI (estimate = -0.00117, SE = 0.00030, t = -3.92, p < 0.0001, 95% CI [-0.00176, -0.00058]). Injury chronicity did not correlate with metabolic alterations. Conclusions: (1)H-MRSI identified regional metabolite-ratio differences in this military TBI cohort. The findings support further investigation of regional Cho/Cr and NAA/Cr measures as correlates of clinical heterogeneity after TBI. The widespread depression of Cho/Cr suggests persistent membrane depletion and/or chronic axonal degeneration, particularly in TBI individuals with multiple blast exposures. Although the cross-sectional design does not establish causality, diagnostic sensitivity, biomechanical strain patterns, or longitudinal recovery, the results imply the utility of MRSI as a robust translational tool for phenotyping chronic military brain injury and objectively monitoring long-term neural recovery. FAU - Yeh, Ping-Hong AU - Yeh PH AUID- ORCID: 0000-0003-0623-7693 AD - Department of Research, National Intrepid Center of Excellence, Walter Reed National Military Medical Center, Bethesda, MD 20889, USA. FAU - Yeh, Chen-Haur AU - Yeh CH AD - Department of Research, National Intrepid Center of Excellence, Walter Reed National Military Medical Center, Bethesda, MD 20889, USA. FAU - Oakes, Terrence R AU - Oakes TR AD - Department of Research, National Intrepid Center of Excellence, Walter Reed National Military Medical Center, Bethesda, MD 20889, USA. FAU - Ollinger, John AU - Ollinger J AUID- ORCID: 0000-0002-2780-9454 AD - Department of Research, National Intrepid Center of Excellence, Walter Reed National Military Medical Center, Bethesda, MD 20889, USA. FAU - Riedy, Gerard AU - Riedy G AD - Department of Research, National Intrepid Center of Excellence, Walter Reed National Military Medical Center, Bethesda, MD 20889, USA. LA - eng GR - CDMRP to USUHS (CNRM) Grant PT074437 and USAMRMC award #13129004./Congressionally Directed Medical Research Programs/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702281 OTO - NOTNLM OT - N-acetylaspartate OT - blast injury OT - choline OT - post-traumatic stress symptoms OT - proton magnetic resonance spectroscopy imaging (1H-MRSI) OT - traumatic brain injury (TBI) COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:33 PHST- 2026/07/01 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - brainsci16101088 [pii] AID - brainsci-16-01088 [pii] AID - 10.3390/brainsci16101088 [doi] PST - epublish SO - Brain Sci. 2026 Oct 9;16(10):1088. doi: 10.3390/brainsci16101088. PMID- 42939332 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 8 TI - Age-Stratified Serum Biomarkers for Discriminating Clinically Defined Cognitive Impairment in Neurological Inpatients: A Cross-Sectional Study. LID - 10.3390/brainsci16101080 [doi] LID - 1080 AB - Background: With the aging of the global population and the increasing prevalence of cognitive dysfunction worldwide, early identification of cognitive impairment has become an important public health issue. This study aims to investigate the correlation between peripheral serum neurodegenerative biomarkers and age, as well as the discriminative efficacy of cognitive impairment across different age cohorts. Notably, subjects were grouped by clinical cognitive assessment without pathological confirmation via amyloid/tau PET or cerebrospinal fluid testing to define Alzheimer's disease pathology. Methods: A cross-sectional study enrolled 425 inpatients from the neurology department. Nonparametric tests were used to explore the correlation between age and peripheral serum biomarkers, as well as intergroup differences in biomarker levels among different cognitive statuses. Age-stratified logistic regression models were constructed to evaluate the association between biomarkers and cognitive impairment, with odds ratios (OR) and 95% confidence intervals (CI) calculated. Receiver operating characteristic (ROC) curves and corresponding area under the curve (AUC) were adopted to assess the discriminative performance of each indicator. In this study, cognitive impairment was defined clinically based on MoCA and clinical assessment, not biomarker-confirmed Alzheimer's disease pathology. Results: Except for glial fibrillary acidic protein (GFAP) (r = 0.062, p = 0.207), all detected biomarkers were significantly correlated with age. Among them, p-Tau181 (r = 0.288, p < 0.001), p-Tau217 (r = 0.275, p < 0.001), Abeta1-42 (r = 0.132, p = 0.007), Abeta1-40 (r = 0.254, p < 0.001), and NfL (r = 0.200, p < 0.001) were positively associated with age, while Abeta42/40 ratio was negatively correlated with age (r = -0.253, p < 0.001). After age-based grouping, phosphorylated tau protein (p-Tau)181 showed relatively better discriminative performance in the <65 years group (AUC = 0.6212, 95% CI: 0.537-0.705); GFAP (AUC = 0.7776, 95% CI: 0.718-0.837) and NfL (AUC = 0.7329, 95% CI: 0.670-0.796) showed higher AUC values among participants aged >/=65 years. Conclusions: The correlation and discriminative efficacy of peripheral serum biomarkers for clinically defined cognitive impairment showed differences across age groups in this exploratory analysis. Within this inpatient cohort, p-Tau181 showed relatively better discriminative performance in the <65 years group, while GFAP and NfL showed numerically higher AUC values in the >/=65 years group. These exploratory findings suggest age-related differences in biomarker performance patterns that warrant further investigation in independent cohorts. The modest AUC (0.6212) for p-Tau181 in the younger group suggests that while it offers a statistical advantage, its clinical utility as a standalone screening tool remains limited, reinforcing the need for comprehensive clinical evaluation. Our results provide exploratory evidence for age-stratified minimally invasive serum-based assessment of clinically defined cognitive impairment in neurological inpatients, and cannot be directly generalized to community populations or used for definite Alzheimer's disease pathological screening; rather, our results serve as a reference for age-stratified screening in clinical inpatient settings rather than standalone diagnostic criteria. FAU - Gu, Dan AU - Gu D AD - Department of Neurology, People's Hospital of Chongqing Liangjiang New Area, Liangjiang Hospital of Chongqing Medical University, No. 2, Jinkai Avenue, Liangjiang New Area, Chongqing 401121, China. AD - Department of Clinical Medicine, Chongqing Medical University, NO.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China. AD - Chongqing Key Laboratory of Neurodegenerative Diseases, No. 118 Xingguang Avenue, Liangjiang New Area, Chongqing 401147, China. FAU - Lei, Yu AU - Lei Y AD - Department of Neurology, People's Hospital of Chongqing Liangjiang New Area, Liangjiang Hospital of Chongqing Medical University, No. 2, Jinkai Avenue, Liangjiang New Area, Chongqing 401121, China. FAU - Lu, Jiayi AU - Lu J AD - Department of Neurology, People's Hospital of Chongqing Liangjiang New Area, Liangjiang Hospital of Chongqing Medical University, No. 2, Jinkai Avenue, Liangjiang New Area, Chongqing 401121, China. AD - Department of Clinical Medicine, Chongqing Medical University, NO.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China. AD - Chongqing Key Laboratory of Neurodegenerative Diseases, No. 118 Xingguang Avenue, Liangjiang New Area, Chongqing 401147, China. FAU - Yi, Wenmin AU - Yi W AD - Department of Neurology, People's Hospital of Chongqing Liangjiang New Area, Liangjiang Hospital of Chongqing Medical University, No. 2, Jinkai Avenue, Liangjiang New Area, Chongqing 401121, China. AD - Department of Clinical Medicine, Chongqing Medical University, NO.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China. AD - Chongqing Key Laboratory of Neurodegenerative Diseases, No. 118 Xingguang Avenue, Liangjiang New Area, Chongqing 401147, China. FAU - Zhang, Qinbin AU - Zhang Q AD - Department of Neurology, People's Hospital of Chongqing Liangjiang New Area, Liangjiang Hospital of Chongqing Medical University, No. 2, Jinkai Avenue, Liangjiang New Area, Chongqing 401121, China. FAU - He, Yuxuan AU - He Y AD - Department of Neurology, People's Hospital of Chongqing Liangjiang New Area, Liangjiang Hospital of Chongqing Medical University, No. 2, Jinkai Avenue, Liangjiang New Area, Chongqing 401121, China. AD - Department of Clinical Medicine, Chongqing Medical University, NO.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China. AD - Chongqing Key Laboratory of Neurodegenerative Diseases, No. 118 Xingguang Avenue, Liangjiang New Area, Chongqing 401147, China. FAU - Wang, Chuanling AU - Wang C AUID- ORCID: 0000-0002-8144-5188 AD - Department of Clinical Medicine, Chongqing Medical University, NO.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China. AD - Department of Pathophysiology, Chongqing Medical University, NO.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China. FAU - Cai, Zhiyou AU - Cai Z AUID- ORCID: 0009-0008-3080-1681 AD - Department of Neurology, People's Hospital of Chongqing Liangjiang New Area, Liangjiang Hospital of Chongqing Medical University, No. 2, Jinkai Avenue, Liangjiang New Area, Chongqing 401121, China. AD - Department of Clinical Medicine, Chongqing Medical University, NO.1 Yixueyuan Road, Yuzhong District, Chongqing 400016, China. AD - Chongqing Key Laboratory of Neurodegenerative Diseases, No. 118 Xingguang Avenue, Liangjiang New Area, Chongqing 401147, China. LA - eng PT - Journal Article DEP - 20261008 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702234 OTO - NOTNLM OT - age OT - cognitive impairment OT - glial fibrillary acidic protein OT - neurofilament light chain OT - tau protein COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:33 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - brainsci16101080 [pii] AID - brainsci-16-01080 [pii] AID - 10.3390/brainsci16101080 [doi] PST - epublish SO - Brain Sci. 2026 Oct 8;16(10):1080. doi: 10.3390/brainsci16101080. PMID- 42939331 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 8 TI - Non-Invasive Brain Stimulation for Central Neuropathic Pain: An Exploratory Randomized Sham-Controlled Crossover Trial. LID - 10.3390/brainsci16101078 [doi] LID - 1078 AB - Background/Objectives: Central neuropathic pain (CNP) is difficult to manage, and comparative evidence for non-invasive brain stimulation is limited. We explored the short-term efficacy and safety of repetitive transcranial magnetic stimulation (rTMS) and transcranial direct current stimulation (tDCS) in CNP. Methods: In this randomized, sham-controlled crossover trial, 26 patients were allocated to active or sham stimulation and received high-frequency rTMS (10 Hz, 2000 pulses/session) and anodal tDCS (2 mA, 30 min) over the primary motor cortex for five days each, separated by a 4-week washout. The primary outcome was change in Numerical Rating Scale (NRS) pain intensity at 1 and 4 weeks, analysed using factorial linear mixed-effects models with and without age adjustment. Results: At 1 week, the difference between active and sham rTMS was 0.76 points (95% CI -0.005 to 1.53; p = 0.051) and 0.42 points after age adjustment (95% CI -0.37 to 1.21; p = 0.30). Active tDCS did not differ from sham tDCS, and active rTMS did not differ from active tDCS. No significant differences were found at 4 weeks or in responder analyses. No serious adverse events occurred. Conclusions: In this small exploratory trial, neither rTMS nor tDCS showed statistically significant superiority over modality-matched sham, and no difference between modalities was demonstrated. Adequately powered trials are needed. FAU - Kim, Sooyoung AU - Kim S AUID- ORCID: 0000-0002-2917-1618 AD - Department of Neurology, Chungnam National University Hospital, Chungnam National University College of Medicine, 282 Munhwa-ro, Jung-gu, Daejeon 35015, Republic of Korea. FAU - Lee, Eun Kyoung AU - Lee EK AUID- ORCID: 0000-0001-7864-527X AD - Department of Neurology, Chungnam National University Sejong Hospital, Chungnam National University College of Medicine, Sejong 30099, Republic of Korea. FAU - Ko, Youngkwon AU - Ko Y AD - Department of Anesthesiology and Pain Medicine, Chungnam National University Hospital, Chungnam National University College of Medicine, Daejeon 35015, Republic of Korea. FAU - Nah, Hyun-Wook AU - Nah HW AUID- ORCID: 0000-0001-6664-1589 AD - Department of Neurology, Chungnam National University Hospital, Chungnam National University College of Medicine, 282 Munhwa-ro, Jung-gu, Daejeon 35015, Republic of Korea. FAU - Jeong, Hye Seon AU - Jeong HS AUID- ORCID: 0000-0002-2031-5662 AD - Department of Neurology, Chungnam National University Hospital, Chungnam National University College of Medicine, 282 Munhwa-ro, Jung-gu, Daejeon 35015, Republic of Korea. FAU - Jeong, Seong-Hae AU - Jeong SH AUID- ORCID: 0000-0001-5055-9986 AD - Department of Neurology, Chungnam National University Hospital, Chungnam National University College of Medicine, 282 Munhwa-ro, Jung-gu, Daejeon 35015, Republic of Korea. FAU - Lee, Ae Young AU - Lee AY AD - Department of Neurology, Chungnam National University Hospital, Chungnam National University College of Medicine, 282 Munhwa-ro, Jung-gu, Daejeon 35015, Republic of Korea. FAU - Sohn, Eunhee AU - Sohn E AUID- ORCID: 0000-0001-5610-7606 AD - Department of Neurology, Chungnam National University Hospital, Chungnam National University College of Medicine, 282 Munhwa-ro, Jung-gu, Daejeon 35015, Republic of Korea. LA - eng GR - 2025-CF-011/Chungnam National University Hospital/ PT - Journal Article DEP - 20261008 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702139 OTO - NOTNLM OT - central neuropathic pain OT - neuromodulation OT - non-invasive brain stimulation OT - repetitive transcranial magnetic stimulation OT - transcranial direct current stimulation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:33 PHST- 2026/09/08 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - brainsci16101078 [pii] AID - brainsci-16-01078 [pii] AID - 10.3390/brainsci16101078 [doi] PST - epublish SO - Brain Sci. 2026 Oct 8;16(10):1078. doi: 10.3390/brainsci16101078. PMID- 42939329 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 7 TI - Latent Multidimensional Organization of White Matter Microstructural Aging: A Generative Adversarial Network Disentanglement Study in a Clinically Defined Reference Cohort. LID - 10.3390/brainsci16101076 [doi] LID - 1076 AB - Background: White matter (WM) aging is commonly characterized as a largely unitary process of progressive microstructural decline, yet substantial interindividual variation suggests that this view may obscure distinct biological dimensions. Whether WM aging retains a multidimensional organization after minimizing variation attributable to overt clinical pathology remains unknown. Methods: We constructed a clinically defined aging reference cohort (CDAR; n = 17,310) from the UK Biobank (UKB) by excluding overt clinical pathology and analyzed 144 skeleton-based WM microstructural metrics. An additional 10,272 participants with overt clinical diagnoses were included for downstream association analyses, yielding a total analytic sample of 27,582. Surreal-GAN was applied to disentangle latent dimensions of WM aging, with reproducibility-based model selection and cross-sample and cross-sex validation. The biological relevance of the identified dimensions was evaluated through associations with cognitive, lifestyle, peripheral, and disease-related outcomes. Results: Three reproducible and coexisting dimensions of WM aging emerged, comprising distinct spatial configurations rather than a single diffuse aging axis. R(1) displayed an anterior-vulnerable/posterior-preserved gradient, R(2) reflected a widespread diffuse aging load, and R(3) showed a dissociation between the limbic-frontal system and sensorimotor pathways. The three patterns exhibited good cross-sample (r = 0.887-0.935) and cross-sex (r = 0.891-0.937) reproducibility, with strong bilateral hemispheric correspondence (r > 0.86). All three patterns were associated with age but exhibited partially shared and partially independent associations with cognition, lifestyle factors, peripheral phenotypes, and disease risk. Conclusions: WM aging is not adequately captured by a single neuroanatomical process. Even after minimizing overt clinical pathology, it retains a reproducible multidimensional organization, with partially shared and partially independent phenotypic profiles across aging dimensions. These findings suggest that individual differences in WM aging reflect partially separable biological processes rather than variation along a single aging axis. FAU - Li, Yanxue AU - Li Y AD - Department of Biomedical Engineering, College of Chemistry and Life Science, Beijing University of Technology, Beijing 100124, China. FAU - Gao, Hongjian AU - Gao H AUID- ORCID: 0000-0002-9902-893X AD - Department of Biomedical Engineering, College of Chemistry and Life Science, Beijing University of Technology, Beijing 100124, China. FAU - Lin, Lan AU - Lin L AUID- ORCID: 0000-0002-3431-2753 AD - Department of Biomedical Engineering, College of Chemistry and Life Science, Beijing University of Technology, Beijing 100124, China. FAU - Xiong, Min AU - Xiong M AD - School of Science, North China University of Technology, Beijing 100144, China. LA - eng GR - Nos.81971683 and 12572064/National Natural Science Foundation of China/ GR - No. L182010/Beijing Municipality Natural Science Foundation/ PT - Journal Article DEP - 20261007 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702479 OTO - NOTNLM OT - UK Biobank OT - brain aging OT - deep learning OT - diffusion MRI OT - heterogeneity OT - influencing factors COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/07 CRDT- 2026/10/27 10:33 PHST- 2026/08/24 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/07 00:00 [pmc-release] AID - brainsci16101076 [pii] AID - brainsci-16-01076 [pii] AID - 10.3390/brainsci16101076 [doi] PST - epublish SO - Brain Sci. 2026 Oct 7;16(10):1076. doi: 10.3390/brainsci16101076. PMID- 42939325 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 6 TI - Maintenance of Auditory Deviance Processing Following Auricular Vagal Neuromodulation Therapy in Disorders of Consciousness. LID - 10.3390/brainsci16101072 [doi] LID - 1072 AB - Background/Objectives: Auricular Vagal Neuromodulation Therapy (AVNT) constitutes a promising non-invasive therapeutic approach for Disorders of Consciousness (DoCs). However, evidence regarding its effectiveness remains limited. In this context, although the Mismatch Negativity (MMN) is a well-established neurophysiological index associated with recovery in DoCs, its modulation by AVNT has not yet been investigated. This study aimed to evaluate the effect of AVNT on MMN in DoCs. Methods: Forty-four participants with DoCs were evenly assigned to either an experimental or a control group, alongside twenty-two healthy participants used to establish normative data in neurotypical conditions. The latency, peak and peak-to-peak MMN amplitude were assessed as a complement to the Coma Recovery Scale Revised (CRS-R). Subsequently, only the experimental group received AVNT, followed by a second assessment across all groups. Results: The control group showed extensive changes across sessions in both peak and peak-to-peak amplitudes. In contrast, the experimental group exhibited a significant change only in peak amplitude for the fronto-central region, with no changes in peak-to-peak amplitude. Between-session changes in peak and peak-to-peak amplitudes differed between the control and healthy groups in the parieto-occipital region. After adjustment for the inter-session interval, the ANCOVA showed an overall group effect on peak-to-peak amplitude changes in the parieto-occipital and right regions. No differences were detected between the control and experimental groups or between the experimental and healthy groups. Finally, no significant effects were found for MMN latency. Conclusions: These findings could be compatible with a possible effect of AVNT, although the evidence remained insufficient to demonstrate a direct effect of the intervention. FAU - Lopez-Rodriguez, Samuel AU - Lopez-Rodriguez S AUID- ORCID: 0000-0002-7169-9045 AD - NEUROCAP Research Group, Psychology Department and Human Neuroscience Laboratory, Faculty of Psychology and Education, Universidad Loyola Andalucia, Avenida de las Universidades, 2, 41704 Dos Hermanas, Spain. FAU - Postigo-Alonso, Barbara AU - Postigo-Alonso B AUID- ORCID: 0000-0002-6265-6027 AD - NEUROCAP Research Group, Psychology Department and Human Neuroscience Laboratory, Faculty of Psychology and Education, Universidad Loyola Andalucia, Avenida de las Universidades, 2, 41704 Dos Hermanas, Spain. FAU - O'Valle-Rodriguez, Myrtha AU - O'Valle-Rodriguez M AD - IRENEA-Instituto de Rehabilitacion Neurologica, C/ de Callosa d'en Sarria, 12, Jesus, 46007 Valencia, Spain. FAU - Gomez-Herranz, Marta AU - Gomez-Herranz M AD - IRENEA-Instituto de Rehabilitacion Neurologica, C/ de Callosa d'en Sarria, 12, Jesus, 46007 Valencia, Spain. FAU - Navarro-Perez, Maria Dolores AU - Navarro-Perez MD AUID- ORCID: 0000-0002-3741-304X AD - IRENEA-Instituto de Rehabilitacion Neurologica, C/ de Callosa d'en Sarria, 12, Jesus, 46007 Valencia, Spain. FAU - Ferri-Campos, Joan AU - Ferri-Campos J AD - IRENEA-Instituto de Rehabilitacion Neurologica, C/ de Callosa d'en Sarria, 12, Jesus, 46007 Valencia, Spain. FAU - Noe-Sebastian, Enrique AU - Noe-Sebastian E AUID- ORCID: 0000-0002-2547-8727 AD - IRENEA-Instituto de Rehabilitacion Neurologica, C/ de Callosa d'en Sarria, 12, Jesus, 46007 Valencia, Spain. FAU - Galvao-Carmona, Alejandro AU - Galvao-Carmona A AUID- ORCID: 0000-0002-5126-1273 AD - Experimental Psychology Department, Faculty of Psychology, University of Seville, 41018 Seville, Spain. LA - eng GR - 101131344/European Commission (MSCA Staff Exchange)/ GR - FPU2022/00195/Spanish Ministry of Science, Innovation and Universities/ PT - Journal Article DEP - 20261006 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702590 OTO - NOTNLM OT - Auricular Vagal Neuromodulation Therapy (AVNT) OT - Coma Recovery Scale Revised (CRS-R) OT - Disorders of Consciousness (DoCs) OT - Minimally Conscious State (MCS) OT - Mismatch Negativity (MMN) OT - Unresponsive Wakefulness Syndrome (UWS) COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/06 CRDT- 2026/10/27 10:33 PHST- 2026/07/16 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/06 00:00 [pmc-release] AID - brainsci16101072 [pii] AID - brainsci-16-01072 [pii] AID - 10.3390/brainsci16101072 [doi] PST - epublish SO - Brain Sci. 2026 Oct 6;16(10):1072. doi: 10.3390/brainsci16101072. PMID- 42939322 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Oct 5 TI - Chronic Inhalation of Sulphureous Water Mitigates Early Amyloid Pathology and Associated Microglial Changes in 5XFAD Mice. LID - 10.3390/brainsci16101069 [doi] LID - 1069 AB - BACKGROUND: Alzheimer's disease (AD) is a chronic neurodegenerative disorder characterized by progressive cognitive decline and behavioral impairments. Hydrogen sulfide (H(2)S) is an endogenous gasotransmitter that exerts pleiotropic neuromodulatory, anti-inflammatory, cytoprotective, and trophic effects within the central nervous system. Objectuves: This study investigated whether chronic inhalation of naturally H(2)S-rich sulphureous water, a non-invasive and clinically applicable intervention, exerts neuroprotective effects in 5XFAD mice, a transgenic model of severe AD pathology. METHODS: Two-month-old male and female wild-type and 5XFAD mice underwent daily inhalation of saline or sulphureous water for eight weeks. Sulphureous water exposure was well tolerated and did not impair baseline learning or memory in either genotype. A detailed analysis of swim trajectories revealed selective enhancement of spatially accurate navigation strategies in sulphureous water-exposed 5XFAD female mice, with nearly a threefold increase in effective search behaviors, whereas male mice showed no treatment-specific effects. In addition, sulphureous water inhalation significantly reduced the number and area of 6E10(+) and 11A1(+) amyloid-beta plaques in corticohippocampal regions and decreased cortical microglia number. Transcriptional profiling of cortical tissue revealed strong genotype-dependent upregulation of microglial, inflammatory, and stress-response genes. Sulphureous water exposure selectively modulated inflammatory and stress-response genes, including Cd11b, Cst, Sod1, Tgfb1, and Hsp90a, with distinct genotype-dependent interactions. RESULTS: Overall, chronic inhalation of naturally H(2)S-rich sulphureous water during the prodromal phase of AD-like pathology mitigated early amyloid burden, reduced cortical microgliosis, and improved qualitative aspects of spatial navigation, particularly in female mice. CONCLUSIONS: These findings warrant further translational investigation of sulphureous water inhalation as a potential preventive or disease-modifying strategy for AD in humans. FAU - Bodria, Martina AU - Bodria M AD - Department of Biomedical, Metabolic and Neural Sciences, Section of Physiology and Neurosciences, University of Modena and Reggio Emilia, 41125 Modena, Italy. FAU - Daini, Eleonora AU - Daini E AUID- ORCID: 0000-0002-4020-1179 AD - Department of Biomedical, Metabolic and Neural Sciences, Section of Physiology and Neurosciences, University of Modena and Reggio Emilia, 41125 Modena, Italy. FAU - Pioli, Stefano AU - Pioli S AUID- ORCID: 0009-0008-9829-6415 AD - Department of Biomedical, Metabolic and Neural Sciences, Section of Pharmacology and Molecular Medicine, University of Modena and Reggio Emilia, 41125 Modena, Italy. FAU - Piemontese, Monica AU - Piemontese M AUID- ORCID: 0009-0002-9236-5842 AD - Department of Biomedical, Metabolic and Neural Sciences, Section of Physiology and Neurosciences, University of Modena and Reggio Emilia, 41125 Modena, Italy. AD - International School of Advanced Studies, University of Camerino, 62032 Camerino, Italy. FAU - Vandini, Eleonora AU - Vandini E AUID- ORCID: 0000-0001-7581-4437 AD - Department of Biomedical, Metabolic and Neural Sciences, Section of Pharmacology and Molecular Medicine, University of Modena and Reggio Emilia, 41125 Modena, Italy. FAU - Ottani, Alessandra AU - Ottani A AUID- ORCID: 0000-0002-1676-051X AD - Department of Biomedical, Metabolic and Neural Sciences, Section of Pharmacology and Molecular Medicine, University of Modena and Reggio Emilia, 41125 Modena, Italy. FAU - Zoli, Michele AU - Zoli M AD - Department of Biomedical, Metabolic and Neural Sciences, Section of Physiology and Neurosciences, University of Modena and Reggio Emilia, 41125 Modena, Italy. FAU - Giuliani, Daniela AU - Giuliani D AD - Department of Biomedical, Metabolic and Neural Sciences, Section of Pharmacology and Molecular Medicine, University of Modena and Reggio Emilia, 41125 Modena, Italy. FAU - Vilella, Antonietta AU - Vilella A AUID- ORCID: 0000-0003-3820-0115 AD - Department of Biomedical, Metabolic and Neural Sciences, Section of Physiology and Neurosciences, University of Modena and Reggio Emilia, 41125 Modena, Italy. LA - eng GR - Fondazione per la Ricerca Scientifica Termale/ GR - MIUR Dipartimenti di Eccellenza 2018-2022/ PT - Journal Article DEP - 20261005 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702382 OTO - NOTNLM OT - Alzheimer's disease OT - H2S-richwater OT - behavior OT - mouse model OT - neuroprotection COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationship that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/05 CRDT- 2026/10/27 10:33 PHST- 2026/08/31 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/05 00:00 [pmc-release] AID - brainsci16101069 [pii] AID - brainsci-16-01069 [pii] AID - 10.3390/brainsci16101069 [doi] PST - epublish SO - Brain Sci. 2026 Oct 5;16(10):1069. doi: 10.3390/brainsci16101069. PMID- 42939315 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Sep 30 TI - Nerve Injuries in Craniofacial and Skull Base Surgery: Shared Mechanisms, Prevention, and Repair Strategies. LID - 10.3390/brainsci16101062 [doi] LID - 1062 AB - BACKGROUND: Craniofacial and skull base procedures place cranial nerves at risk of injury, with consequences for movement, sensation, hearing, swallowing, vision, and quality of life. METHODS: This narrative review integrates 46 original publications with six targeted references added during revision, addressing injury mechanisms, prevention, postoperative assessment, reconstruction, and rehabilitation across neurosurgery, otolaryngology, and maxillofacial surgery. RESULTS: Recurring mechanisms include traction, compression, ischemia, thermal injury, direct trauma, and transection. Neuromonitoring contributes to localization, functional surveillance, and prognosis when paired with reproducible baseline recordings, appropriate modality selection, technical troubleshooting, and a predefined surgical response to alerts. Preoperative and postoperative electrophysiological testing complements but does not replace intraoperative monitoring. Recognized transection favors immediate tension-free reconstruction when feasible; an anatomically continuous nerve with an unfavorable electrical response requires contextual interpretation rather than automatic sacrifice. Rehabilitation combines target-organ protection, selective motor or sensory retraining, symptom control, and patient-reported assessment. CONCLUSIONS: The practical distinction is between a potentially reversible functional disturbance and confirmed structural discontinuity. Management should link monitoring changes to corrective action, preserve viable nerves, and coordinate timely reconstruction with rehabilitation. Heterogeneous, predominantly observational evidence does not support universal alert thresholds, a single reconstruction deadline, or guaranteed functional recovery. FAU - Chatzistefanou, Ioannis AU - Chatzistefanou I AUID- ORCID: 0000-0001-7463-6106 AD - University General Hospital "Attikon", National and Kapodistrian University of Athens, 12462 Athens, Greece. FAU - Tsokkou, Sophia AU - Tsokkou S AUID- ORCID: 0009-0000-1703-6980 AD - Department of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. FAU - Liatsos, Alexandros C AU - Liatsos AC AUID- ORCID: 0009-0005-1715-1272 AD - Department of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. FAU - Tsolkas, Panagiotis AU - Tsolkas P AD - Department of Oral and Maxillofacial Surgery, Athens Naval Hospital, 11521 Athens, Greece. FAU - Papadopoulou, Kyriaki AU - Papadopoulou K AUID- ORCID: 0000-0002-6138-0970 AD - Department of Neurosurgery, AHEPA University Hospital, Aristotle University of Thessaloniki, Kiriakidi 1, 54636 Thessaloniki, Greece. FAU - Florou, Maria AU - Florou M AUID- ORCID: 0000-0002-2166-4338 AD - Second Department of Pediatric Surgery, "Papageorgiou" General Hospital, Aristotle University of Thessaloniki, 54636 Thessaloniki, Greece. FAU - Pyraki, Martha AU - Pyraki M AD - University General Hospital "Attikon", National and Kapodistrian University of Athens, 12462 Athens, Greece. FAU - Papamitsou, Theodora AU - Papamitsou T AUID- ORCID: 0000-0002-0709-5407 AD - Laboratory of Histology-Embryology, Department of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece. LA - eng PT - Journal Article PT - Review DEP - 20260930 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702288 OTO - NOTNLM OT - cranial nerve injury OT - craniofacial surgery OT - facial nerve OT - facial reanimation OT - inferior alveolar nerve OT - intraoperative neuromonitoring OT - nerve repair OT - skull base surgery OT - trigeminal nerve COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:33 PHST- 2026/09/07 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - brainsci16101062 [pii] AID - brainsci-16-01062 [pii] AID - 10.3390/brainsci16101062 [doi] PST - epublish SO - Brain Sci. 2026 Sep 30;16(10):1062. doi: 10.3390/brainsci16101062. PMID- 42939313 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Sep 30 TI - Siponimod Induces Peripheral T Cell Redistribution Without Affecting Glial Cells or Behavior in Naive Mice. LID - 10.3390/brainsci16101060 [doi] LID - 1060 AB - Background: Siponimod is a selective modulator of sphingosine-1-phosphate receptors 1 and 5, approved for the treatment of secondary progressive multiple sclerosis. Apart from its immunomodulatory effects, it has been proposed to exert pro-regenerative and pro-myelinating effects. However, it is unclear whether the reported neuroprotective and pro-regenerative effects of siponimod reflect direct engagement on glial cells or arise secondary to reduced neuroinflammation. Methods: We investigated the systemic and central effects of siponimod in naive mice treated orally with 3 mg/kg for 19 consecutive days. Peripheral immune cell populations were analyzed by flow cytometry, drug exposure was quantified by liquid chromatography and high-resolution mass spectrometry, glial cell populations were assessed by immunohistochemistry, and functional outcomes were evaluated using behavioral tests. Results: Siponimod treatment induced pronounced T cell lymphopenia, characterized by reduced CD8(+) T cells and increased proportions of double-negative T cells and CD4(+)CD25(+) T cells. Lymphocyte distribution was compartment-specific, with increased splenic T cell and reduced thymic T cell counts, while lymph nodes were largely unaffected. Siponimod penetrated the CNS, with hippocampal concentrations exceeding plasma levels. Despite robust brain exposure, siponimod did not affect oligodendrocyte lineage cell density, proliferation, microglial cell density, or morphology, nor did it affect behavior in mice. Conclusions: Taken together, these findings demonstrate that under physiological conditions, the actions of siponimod lie primarily in its immunomodulatory effects, while direct effects on glial cells and behavior are absent. Furthermore, this study paves the way for studies of siponimod's effect on glial cells and neurons in models with minimal or no blood-brain barrier damage. FAU - Jakobsen, Julie Damgaard Rosgaard AU - Jakobsen JDR AUID- ORCID: 0000-0001-8771-3107 AD - Department of Neurology, Odense University Hospital, 5000 Odense, Denmark. AD - Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, 5000 Odense, Denmark. AD - BRIDGE-Brain Research Inter-Disciplinary Guided Excellence, Department of Clinical Research, University of Southern Denmark, 5000 Odense, Denmark. FAU - Thougaard, Estrid AU - Thougaard E AD - Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, 5000 Odense, Denmark. FAU - Becirovic, Lejla Vahl AU - Becirovic LV AD - Department of Neurology, Odense University Hospital, 5000 Odense, Denmark. AD - Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, 5000 Odense, Denmark. AD - BRIDGE-Brain Research Inter-Disciplinary Guided Excellence, Department of Clinical Research, University of Southern Denmark, 5000 Odense, Denmark. FAU - Phuong, Victoria AU - Phuong V AD - Department of Neurology, Odense University Hospital, 5000 Odense, Denmark. AD - Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, 5000 Odense, Denmark. FAU - Jensen, Amalie Forsberg AU - Jensen AF AD - Department of Neurology, Odense University Hospital, 5000 Odense, Denmark. AD - Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, 5000 Odense, Denmark. FAU - Wellinghof, Tim AU - Wellinghof T AD - Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, 5000 Odense, Denmark. FAU - Nielsen, Pernille Vinther AU - Nielsen PV AD - Department of Neurology, Odense University Hospital, 5000 Odense, Denmark. AD - Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, 5000 Odense, Denmark. AD - BRIDGE-Brain Research Inter-Disciplinary Guided Excellence, Department of Clinical Research, University of Southern Denmark, 5000 Odense, Denmark. FAU - Raffaele, Stefano AU - Raffaele S AUID- ORCID: 0000-0002-2734-5418 AD - Department of Pharmaceutical Sciences, Universita degli Studi di Milano, 20122 Milano, Italy. FAU - Fumagalli, Marta AU - Fumagalli M AUID- ORCID: 0000-0002-0158-842X AD - Department of Pharmaceutical Sciences, Universita degli Studi di Milano, 20122 Milano, Italy. FAU - Fex Svenningsen, Asa AU - Fex Svenningsen A AUID- ORCID: 0000-0002-5651-265X AD - Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, 5000 Odense, Denmark. AD - BRIDGE-Brain Research Inter-Disciplinary Guided Excellence, Department of Clinical Research, University of Southern Denmark, 5000 Odense, Denmark. FAU - Finsen, Bente AU - Finsen B AD - Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, 5000 Odense, Denmark. AD - BRIDGE-Brain Research Inter-Disciplinary Guided Excellence, Department of Clinical Research, University of Southern Denmark, 5000 Odense, Denmark. FAU - Wlodarczyk, Agnieszka AU - Wlodarczyk A AUID- ORCID: 0000-0003-4123-368X AD - Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, 5000 Odense, Denmark. AD - BRIDGE-Brain Research Inter-Disciplinary Guided Excellence, Department of Clinical Research, University of Southern Denmark, 5000 Odense, Denmark. FAU - Nielsen, Flemming AU - Nielsen F AUID- ORCID: 0000-0002-5657-405X AD - Clinical Pharmacology, Pharmacy and Environmental Medicine, Department of Public Health, University of Southern Denmark, 5000 Odense, Denmark. FAU - Lambertsen, Kate Lykke AU - Lambertsen KL AUID- ORCID: 0000-0001-9276-4902 AD - Department of Neurology, Odense University Hospital, 5000 Odense, Denmark. AD - Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, 5000 Odense, Denmark. AD - BRIDGE-Brain Research Inter-Disciplinary Guided Excellence, Department of Clinical Research, University of Southern Denmark, 5000 Odense, Denmark. FAU - Nielsen, Helle Hvilsted AU - Nielsen HH AD - Department of Neurology, Odense University Hospital, 5000 Odense, Denmark. AD - Department of Neurobiology Research, Institute of Molecular Medicine, University of Southern Denmark, 5000 Odense, Denmark. AD - BRIDGE-Brain Research Inter-Disciplinary Guided Excellence, Department of Clinical Research, University of Southern Denmark, 5000 Odense, Denmark. LA - eng GR - Mrs. Lily Benthine Lunds Foundation/ GR - Horslevsfonden/ GR - Direktor Ejnar Johnsens Mindelegat/ GR - AJ Andersens Fond/ GR - CF21-0215/Carlsberg Foundation/ GR - BRIDGE at the Faculty of Health Sciences, University of Southern Denmark/ GR - Familien Hede Nielsens Fond/ GR - Lizzy and Mogens Staal Fonden/ GR - The Danish Multiple Sclerosis Society/ PT - Journal Article DEP - 20260930 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702611 OTO - NOTNLM OT - T cell lymphopenia OT - lymphocyte trafficking OT - neuroimmunology OT - siponimod OT - sphingosine-1-phosphate receptor COIS- JDRJ, TW, PVN, VP, AFJ, ET, LVB, AW, SR, MF, AFS, BF, FN, and KL: nothing to declare; HHN: HHN has served on advisory boards and participated in meetings and conferences sponsored by Merck A/S, Novartis AG, Roche A/S, Neuraxpharm AB, and Sanofi A/S. The funders had no role in the design of the study; in the collection, analysis, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:33 PHST- 2026/09/03 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - brainsci16101060 [pii] AID - brainsci-16-01060 [pii] AID - 10.3390/brainsci16101060 [doi] PST - epublish SO - Brain Sci. 2026 Sep 30;16(10):1060. doi: 10.3390/brainsci16101060. PMID- 42939308 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Sep 30 TI - Impact of Editor's Choice Selections on Review Articles in the "Sensory and Motor Neuroscience" Section of Brain Sciences in 2025. LID - 10.3390/brainsci16101055 [doi] LID - 1055 AB - The leading review articles for 2025 are clustered [...]. FAU - Murphy, Bernadette AU - Murphy B AUID- ORCID: 0000-0001-9832-5740 AD - Faculty of Health Sciences, Ontario Tech University, Oshawa, ON L1G 0C5, Canada. LA - eng PT - Editorial DEP - 20260930 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702148 COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:33 PHST- 2026/08/27 00:00 [received] PHST- 2026/08/28 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - brainsci16101055 [pii] AID - brainsci-16-01055 [pii] AID - 10.3390/brainsci16101055 [doi] PST - epublish SO - Brain Sci. 2026 Sep 30;16(10):1055. doi: 10.3390/brainsci16101055. PMID- 42939307 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Sep 30 TI - Comparative Effects of Acute HIIE and MICE on Executive Function, Prefrontal Oxyhaemoglobin Responses, and Related Biomarkers in Sedentary Young Adults: A Randomized Crossover Trial. LID - 10.3390/brainsci16101054 [doi] LID - 1054 AB - BACKGROUND/OBJECTIVES: To compare the acute effects of high-intensity interval exercise (HIIE) and moderate-intensity continuous exercise (MICE) on executive-function performance, prefrontal oxyhaemoglobin (Oxy-Hb) signals, brain-derived neurotrophic factor (BDNF), and lactate in sedentary young adults. METHODS: Fifteen participants (10 men, 5 women; 22 +/- 2 years; BMI 23.07 +/- 4.12 kg/m(2)) completed HIIE, MICE, and seated control sessions in randomized order with 7-day washouts. Stroop, N-back, and Switching tasks; prefrontal fNIRS; plasma BDNF; and blood lactate were assessed before and after each condition. Correlation and indirect-effect analyses were exploratory. RESULTS: Both HIIE and MICE significantly improved inhibitory control, working memory, and cognitive flexibility relative to baseline. For inhibitory control, both exercise modes reduced reaction times in congruent and incongruent Stroop tasks, but only HIIE improved Stroop interference performance. Both HIIE and MICE improved inhibitory control, working memory, and cognitive flexibility relative to baseline. Both reduced reaction times in the congruent and incongruent Stroop, 3-back, and Switching trials, whereas only HIIE improved Stroop interference. Both exercise modes increased Oxy-Hb in the OFA during Stroop and in the FPA and R-DLPFC during Switching. HIIE additionally increased Oxy-Hb in the FPA during Stroop, PTR during Switching, and R-DLPFC during N-back. Both exercise modes increased circulating BDNF and lactate, with a greater lactate response after HIIE. Improvements in executive function were associated with changes in Oxy-Hb, BDNF, and lactate. CONCLUSIONS: Both exercise sessions were followed by faster reaction times on selected tasks. The small sample, multiple analyses, and potential systemic contributions to fNIRS signals preclude firm conclusions about exercise-mode superiority or biological mediation. FAU - Huang, Junhao AU - Huang J AD - Guangdong Provincial Key Laboratory of Physical Activity and Health Promotion, Guangzhou Sport University, 1268 Middle Guangzhou Avenue, Guangzhou 510500, China. FAU - Huang, Jinglin AU - Huang J AD - Guangdong Provincial Key Laboratory of Physical Activity and Health Promotion, Guangzhou Sport University, 1268 Middle Guangzhou Avenue, Guangzhou 510500, China. FAU - Liu, Ziqing AU - Liu Z AD - Guangdong Provincial Key Laboratory of Physical Activity and Health Promotion, Guangzhou Sport University, 1268 Middle Guangzhou Avenue, Guangzhou 510500, China. FAU - Yang, Xinyue AU - Yang X AUID- ORCID: 0009-0008-5572-132X AD - Guangdong Provincial Key Laboratory of Physical Activity and Health Promotion, Guangzhou Sport University, 1268 Middle Guangzhou Avenue, Guangzhou 510500, China. AD - School of Exercise and Health, Shanghai University of Sport, Shanghai 200438, China. LA - eng GR - 2023ZDZX2033/Department of Education of Guangdong Province/ GR - 2025WCXTD012/Department of Education of Guangdong Province/ PT - Journal Article DEP - 20260930 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702242 OTO - NOTNLM OT - executive function OT - exercise OT - fNIRS OT - sedentary behaviour COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:33 PHST- 2026/08/20 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - brainsci16101054 [pii] AID - brainsci-16-01054 [pii] AID - 10.3390/brainsci16101054 [doi] PST - epublish SO - Brain Sci. 2026 Sep 30;16(10):1054. doi: 10.3390/brainsci16101054. PMID- 42939305 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Sep 30 TI - Non-Invasive Brain Stimulation Techniques and Effects on Neuromodulation and Athletic Performance in Combat Sports: A Systematic Review. LID - 10.3390/brainsci16101052 [doi] LID - 1052 AB - BACKGROUND: Non-invasive brain stimulation (NIBS), including transcranial direct current stimulation (tDCS) and transcranial magnetic stimulation (TMS), has emerged as a potential ergogenic strategy for modulating neural activity and enhancing cognitive and physical performance in sports. However, evidence regarding its application in combat sports remains limited, heterogeneous, and fragmented. This study aimed to systematically synthesize the effects of NIBS on neuromodulation and cognitive, physical, and psychophysiological performance-related outcomes in combat sport athletes. Although NIBS encompasses several stimulation techniques, the evidence identified in combat sport athletes in this review predominantly involved tDCS. METHODS: A systematic review was conducted in accordance with the PRISMA 2020 guidelines and registered in PROSPERO (CRD420251150915). Searches were performed in CAPES, PubMed, Scopus, and SPORTDiscus using predefined keywords related to combat sports and brain stimulation techniques. Studies were included if they involved combat sport athletes and investigated the effects of NIBS on performance-related outcomes. Data extraction included study design, sample characteristics, stimulation protocols, and main findings. Methodological quality was assessed using the Cochrane Risk of Bias 2 (RoB 2) tool for randomized studies and ROBINS-I for non-randomized studies, while certainty of evidence was evaluated using the GRADE framework. RESULTS: Nine studies were included in the final synthesis (eight identified through database searching and one through other methods/manual reference screening), encompassing athletes from taekwondo, judo, boxing, mixed martial arts, fencing, and Brazilian jiu-jitsu. Most studies applied tDCS protocols targeting the primary motor cortex (M1) or the dorsolateral prefrontal cortex (DLPFC), with intensities ranging from 1 to 2 mA and stimulation durations between 10 and 20 min. tDCS demonstrated potential benefits in reaction time, selective attention, memory, and resistance to fatigue, as reflected by increased time to task failure. Additionally, enhancements in neuromuscular activation and reductions in anxiety were observed. However, results were heterogeneous, and some studies reported no significant effects on performance-related variables. Risk-of-bias assessment revealed methodological limitations across studies, and GRADE indicated low-to-very-low certainty of evidence for most outcomes. CONCLUSIONS: tDCS may influence selected cognitive, neuromuscular, and fatigue-related outcomes in combat sport athletes; however, the direction and magnitude of these effects are inconsistent across studies. Given the substantial methodological heterogeneity and the low-to-very-low certainty of evidence, no definitive conclusions regarding efficacy or routine practical application can currently be drawn. Further large-scale, standardized, high-quality randomized controlled trials are needed. FAU - Teixeira, Rafael Pereira Azevedo AU - Teixeira RPA AD - Laboratory of Psychophysiology and Performance in Sports and Combats, Department of Physical Education, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, 540-Cidade Universitaria, Rio de Janeiro 21941-599, RJ, Brazil. FAU - Muller, Vanessa Teixeira AU - Muller VT AD - Laboratory of Psychophysiology and Performance in Sports and Combats, Department of Physical Education, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, 540-Cidade Universitaria, Rio de Janeiro 21941-599, RJ, Brazil. FAU - Lopes, Daniel Rodrigues AU - Lopes DR AUID- ORCID: 0009-0004-8942-8392 AD - Laboratory of Psychophysiology and Performance in Sports and Combats, Department of Physical Education, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, 540-Cidade Universitaria, Rio de Janeiro 21941-599, RJ, Brazil. FAU - Freitas, Marcson Love Maquine de AU - Freitas MLM AD - Laboratory of Psychophysiology and Performance in Sports and Combats, Department of Physical Education, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, 540-Cidade Universitaria, Rio de Janeiro 21941-599, RJ, Brazil. FAU - Herzog, Maycon da Cunha AU - Herzog MDC AD - Laboratory of Psychophysiology and Performance in Sports and Combats, Department of Physical Education, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, 540-Cidade Universitaria, Rio de Janeiro 21941-599, RJ, Brazil. FAU - Soto, Dany Alexis Sobarzo AU - Soto DAS AUID- ORCID: 0000-0001-8479-7149 AD - Magister en Ciencias la Actividad Fisica y Deportes Aplicadas al Entrenamiento Rehabilitacion y Reintegro Deportivo, Universidad Santo Tomas, Puerto Montt 5500000, Chile. FAU - Brito, Ciro Jose AU - Brito CJ AUID- ORCID: 0000-0002-9678-1977 AD - Programa de Pos-Graduacao em Educacao Fisica, Departamento de Educacao Fisica, Universidade Federal de Vicosa, Vicosa 36570-900, Brazil. FAU - Miarka, Bianca AU - Miarka B AUID- ORCID: 0000-0002-7513-7605 AD - Laboratory of Psychophysiology and Performance in Sports and Combats, Department of Physical Education, Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, 540-Cidade Universitaria, Rio de Janeiro 21941-599, RJ, Brazil. LA - eng PT - Journal Article PT - Review DEP - 20260930 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702376 OTO - NOTNLM OT - athletic performance OT - cognitive performance OT - martial arts OT - motor performance OT - neuromodulation OT - reaction time OT - sports neuroscience COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/30 CRDT- 2026/10/27 10:33 PHST- 2026/06/19 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - brainsci16101052 [pii] AID - brainsci-16-01052 [pii] AID - 10.3390/brainsci16101052 [doi] PST - epublish SO - Brain Sci. 2026 Sep 30;16(10):1052. doi: 10.3390/brainsci16101052. PMID- 42939302 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-3425 (Print) IS - 2076-3425 (Electronic) IS - 2076-3425 (Linking) VI - 16 IP - 10 DP - 2026 Sep 29 TI - TMEM164 mRNA in Adult Glioblastoma: Multicohort Overexpression and RIPK1 Co-Expression Without Prognostic Replication. LID - 10.3390/brainsci16101049 [doi] LID - 1049 AB - Background/Objectives: Experimental work has implicated TMEM164 in glioblastoma radioresistance through post-translational inhibition of FASN and suppression of radiation-induced necroptosis, supported clinically by a dichotomised single-database survival analysis. We tested whether the previously reported transcript-level clinical association of TMEM164 is reproducible across independent human glioblastoma cohorts and characterised its relationship with tumour status, necroptosis-related transcripts and recurrence. Methods: Secondary analysis of four public resources: TCGA (n = 153), CGGA-693 (n = 104), CGGA-325 (n = 73), and 104 matched primary-recurrence pairs from GLASS. Overall survival was modelled by continuous Cox regression, pooled by Hartung-Knapp random-effects meta-analysis. Results:TMEM164 occupied a higher within-sample percentile in tumour than in non-tumour brains in all three cohorts (Cliff's delta 0.68-0.85; all p /=50% score reduction from baseline) were achieved by 50.0% of participants for depression (BDI) and 41.7% for anxiety (BAI), with 25.0% demonstrating a concurrent dual response on both measures. Exploratory profiling and multivariable continuous modeling indicated that higher cumulative improvement was descriptively observed in older participants with greater baseline symptom severity, with baseline anxiety showing the strongest continuous standardized association (beta = 0.548, p = 0.104). These are hypothesis-generating observations only. Conclusions: The combined three-component package (tDCS, tcVNS, and concurrent supportive counseling) appears feasible, well tolerated, and associated with pre-post symptom reductions that, in the absence of a control condition, cannot be causally attributed to the intervention, warranting further investigation in adequately powered randomized sham-controlled trials. FAU - Marchis, Maria AU - Marchis M AUID- ORCID: 0009-0005-6401-3729 AD - Faculty of Psychology and Educational Sciences, "Alexandru Ioan Cuza" University, 700554 Iasi, Romania. AD - Faculty of Medicine, "Grigore T. Popa" University of Medicine and Pharmacy, 700115 Iasi, Romania. FAU - Iorga, Magdalena AU - Iorga M AUID- ORCID: 0000-0002-7074-959X AD - Faculty of Psychology and Educational Sciences, "Alexandru Ioan Cuza" University, 700554 Iasi, Romania. AD - Faculty of Medicine, "Grigore T. Popa" University of Medicine and Pharmacy, 700115 Iasi, Romania. FAU - Mihaianu, Iustina-Gabriela AU - Mihaianu IG AUID- ORCID: 0009-0008-9257-8104 AD - Faculty of Medicine, "Grigore T. Popa" University of Medicine and Pharmacy, 700115 Iasi, Romania. FAU - Apostol, Maria-Manuela AU - Apostol MM AUID- ORCID: 0009-0004-6094-7017 AD - Faculty of Psychology and Educational Sciences, "Alexandru Ioan Cuza" University, 700554 Iasi, Romania. AD - Faculty of Medicine, "Grigore T. Popa" University of Medicine and Pharmacy, 700115 Iasi, Romania. AD - Prolife Clinics, 700020 Iasi, Romania. LA - eng PT - Journal Article DEP - 20260922 PL - Switzerland TA - Brain Sci JT - Brain sciences JID - 101598646 PMC - PMC13702339 OTO - NOTNLM OT - anxiety OT - depression OT - neuromodulation OT - transcranial direct current stimulation OT - transcutaneous cervical vagus nerve stimulation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/09/22 CRDT- 2026/10/27 10:33 PHST- 2026/09/03 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/20 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/09/22 00:00 [pmc-release] AID - brainsci16101004 [pii] AID - brainsci-16-01004 [pii] AID - 10.3390/brainsci16101004 [doi] PST - epublish SO - Brain Sci. 2026 Sep 22;16(10):1004. doi: 10.3390/brainsci16101004. PMID- 42939252 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 21 TI - Free-Ranging Cat Predation on Wildlife in East Asia: A Scoping Review of Ecological Evidence and Knowledge Gaps. LID - 10.3390/ani16203321 [doi] LID - 3321 AB - Free-ranging domestic cats (Felis catus) interact with wildlife through predation and other ecological pathways, yet evidence of their associated effects has not been comprehensively synthesized in East Asia. This scoping review mapped empirical evidence on free-ranging cat predation and associated ecological effects in East Asia, characterized its geographic distribution and inferential strength, and identified knowledge gaps. Five databases, including region-specific databases, were searched through June 2026, and reference lists of included full-text sources were hand-searched. Of 9326 records identified, 31 sources met the inclusion criteria: 28 from the pre-specified East Asian region and 3 global or multinational sources with independently extractable East Asia-specific data. Diet- and carcass-based studies documented consumption of diverse wildlife taxa, whereas fewer evaluated prey abundance or other population-level outcomes. Reported predation estimates varied substantially among studies, reflecting differences in cat category, ecological setting, and methodology. Evidence was concentrated in Japan, with the clearest conservation concerns reported from Japanese island ecosystems involving endemic or IUCN-listed threatened species. Human-mediated factors and management practices were recurrent contextual themes; however, direct evidence linking these factors to wildlife outcomes was limited. Overall, the evidence base was geographically and methodologically uneven, underscoring the need for standardization, broader regional coverage, and improved documentation. FAU - Zhou, Michelle Zhibing AU - Zhou MZ AUID- ORCID: 0009-0002-7290-1636 AD - Cranbrook Schools, Bloomfield Hills, MI 48304, USA. FAU - Wright, Christian S AU - Wright CS AD - Berkwood Research Institute, Santa Barbara, CA 93101, USA. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700886 OTO - NOTNLM OT - East Asia OT - Felis catus OT - biodiversity OT - invasive species OT - island ecology OT - predation OT - prey populations OT - scoping review OT - trap-neuter-return OT - wildlife conservation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:33 PHST- 2026/08/07 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:33 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ani16203321 [pii] AID - animals-16-03321 [pii] AID - 10.3390/ani16203321 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 21;16(20):3321. doi: 10.3390/ani16203321. PMID- 42939249 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 21 TI - Genome-Wide Detection of Genetic Diversity and Genomic Structure in Hanwoo Cows. LID - 10.3390/ani16203320 [doi] LID - 3320 AB - This study aimed to utilize genomic information to elucidate the genetic diversity and structure of Hanwoo cows and develop a more efficient improvement system. Genomic DNA was extracted from hair root samples of 7273 Hanwoo cows, and genotypes were determined using a customized Illumina Hanwoo SNP50k BeadChip. A total of 41,884 single-nucleotide polymorphisms (SNPs) covering approximately 2500 Mb of the genome were selected for analysis, along with 7166 Hanwoo cows that passed quality control. The mean observed and expected heterozygosity were 0.358 +/- 0.125 and 0.357 +/- 0.124, respectively. A total of 94,560 runs of homozygosity (ROHs) were observed, with an average of 13.2 per animal. Linkage disequilibrium (LD) was estimated using two parameters, D' and r(2), which were found to be 0.381 +/- 0.309 and 0.054 +/- 0.126, respectively, on autosomes. LD declined sharply from< 50 kb to 50-100 kb. In addition, 1818 haplotype blocks were identified on autosomes, totaling 88.27 Mb (3.53% of the autosomal length). The most common haplotype block size was two SNPs, with 1101 blocks. Finally, the effective population size (Ne) was 2853 at 1691 generations but decreased to 277 at 13 generations. These results provide genomic information for further studies and will be helpful for Hanwoo breeding programs. FAU - Oh, Seungwon AU - Oh S AUID- ORCID: 0000-0003-4547-3779 AD - Department of Animal Science and Biotechnology, Graduate School, Kyungpook National University, Sangju 37224, Republic of Korea. FAU - Yoon, Duhak AU - Yoon D AD - Department of Animal Science and Biotechnology, Graduate School, Kyungpook National University, Sangju 37224, Republic of Korea. AD - Research Institute for Innovative Animal Science, Kyungpook National University, Sangju 37224, Republic of Korea. LA - eng GR - RS-2025-25415283/National Research Foundation of Korea/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700702 OTO - NOTNLM OT - Hanwoo cow OT - genetic diversity OT - linkage disequilibrium OT - runs of homozygosity OT - single nucleotide polymorphism COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:32 PHST- 2026/09/28 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ani16203320 [pii] AID - animals-16-03320 [pii] AID - 10.3390/ani16203320 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 21;16(20):3320. doi: 10.3390/ani16203320. PMID- 42939248 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 21 TI - Genome-Wide Association Study of Body Conformation and Carcass Composition Traits in Landrace Pigs. LID - 10.3390/ani16203316 [doi] LID - 3316 AB - Body conformation and carcass composition are economically important traits in pig production, yet their underlying genetic mechanisms remain incompletely characterized. To dissect the genetic bases of body length (BL), body height (BH), backfat thickness (BFT), and lean meat percentage (LMP), we estimated genetic parameters and performed a genome-wide association study (GWAS) using 50 K SNP array genotypes from 1527 Landrace pigs. Phenotypic variance decomposition revealed moderate-to-high heritability for all four traits, ranging from 0.23 for BH to 0.43 for BL. Genetic correlation analysis uncovered a strong negative correlation (-0.80 +/- 0.05) between BFT and LMP, as well as a moderate positive correlation (0.46 +/- 0.03) between BL and BH. Our GWAS identified 14 SNP-trait associations involving 10 unique SNPs and two genomic regions with overlapping association signals. On Sus scrofa chromosome 1 (SSC1), overlapping signals for BFT and LMP occurred near ABL1 and QRFP, whereas signals for body conformation mapped to SSC17 near BMP2, PROKR2, SLC23A2, and PCNA. These genes are positional and biologically plausible candidates, and these candidate markers hold potential for genomic selection to simultaneously improve body size and meat-production performance. FAU - Deng, Shaoxiong AU - Deng S AD - State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China. FAU - Ruan, Donglin AU - Ruan D AD - State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China. FAU - Pu, Zilun AU - Pu Z AD - State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China. FAU - Cao, Zhibin AU - Cao Z AD - State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China. FAU - Huang, Linhao AU - Huang L AD - State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China. FAU - Lin, Meng AU - Lin M AD - State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China. AD - National and Regional Livestock Genebank, Guangdong Gene Bank of Livestock and Poultry, South China Agricultural University, Guangzhou 510642, China. FAU - Wu, Jia-Jin AU - Wu JJ AD - State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China. AD - Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, China. FAU - Zheng, Enqin AU - Zheng E AD - State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China. AD - Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, China. FAU - Yang, Jie AU - Yang J AUID- ORCID: 0000-0002-7031-2160 AD - State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China. AD - National and Regional Livestock Genebank, Guangdong Gene Bank of Livestock and Poultry, South China Agricultural University, Guangzhou 510642, China. AD - Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, China. FAU - Liu, Langqing AU - Liu L AD - State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China. AD - National and Regional Livestock Genebank, Guangdong Gene Bank of Livestock and Poultry, South China Agricultural University, Guangzhou 510642, China. AD - Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, China. FAU - Zhang, Hui AU - Zhang H AUID- ORCID: 0000-0003-0468-3526 AD - State Key Laboratory of Swine and Poultry Breeding Industry, College of Animal Science and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China. AD - School of Life Science, Anhui Medical University, Hefei 230032, China. LA - eng GR - 2024A1515010967/Guangdong Basic and Applied Basic Research Foundation/ GR - 2026-XPY-01-002/Guangdong Provincial Special Project on Seed Industry Revitalization/ GR - NYQN2025004/Guangdong Special Support Program-Platform of the Provincial Department of Agriculture and Rural Affairs (Agricultural and Rural Revitalization Talents) Project/ GR - 2023B10564001 to L.L./South China Agricultural University discipline construction and development project/ GR - 2408085QH259 to H.Z./Natural Science Foundation of Anhui Province/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700893 OTO - NOTNLM OT - GWAS OT - Landrace pig OT - body conformation OT - carcass composition COIS- The authors declare no conflicts of interest. One pending patent application (application no. 202611561065.2, filed on 10 October 2026, South China Agricultural University) related to the SNP marker reported in this study has been disclosed in the Section 5. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:32 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ani16203316 [pii] AID - animals-16-03316 [pii] AID - 10.3390/ani16203316 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 21;16(20):3316. doi: 10.3390/ani16203316. PMID- 42939245 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 21 TI - Effects of Thymol Supplementation in Health Care Sand on Growth Performance, Serum Biochemical Parameters, Fecal Microbial Diversity, and Fecal Metabolites in Squabs. LID - 10.3390/ani16203314 [doi] LID - 3314 AB - This study aimed to investigate the effects of thymol supplementation in health care sand on growth performance, serum biochemical parameters, fecal microbial diversity, and fecal metabolites in Grey King squabs. A total of 160 healthy 28-day-old Grey King squabs were selected and randomly assigned to four treatment groups: the CON, 2%Thy, 3%Thy, and 4%Thy groups. Each group consisted of 10 replicates with four squabs per replicate, resulting in a total of 40 squabs per group. Under identical feeding management conditions and dietary nutrient levels, squabs in the CON group received the basal diet plus 5 g of health care sand; those in the 2%Thy group received the basal diet plus 5 g of health care sand containing 2% thymol; those in the 3%Thy group received the basal diet plus 5 g of health care sand containing 3% thymol; and those in the 4%Thy group received the basal diet plus 5 g of health care sand containing 4% thymol. The experiment lasted for 30 days. During the experimental period, the pigeons were housed in cages measuring 45 cm x 50 cm x 60 cm. Each pigeon was provided with 40 g of diet and 5 g of health care sand daily and had free access to water. After Benjamini-Hochberg false discovery rate correction, no significant differences were observed in growth performance, body measurements, apparent nutrient retention, or energy metabolizability among treatments (q > 0.05). However, thymol-supplemented groups exhibited significantly increased serum total antioxidant capacity and superoxide dismutase, glutathione peroxidase, and catalase activities, together with decreased malondialdehyde levels (q < 0.05). Fecal microbiome and metabolomic analyses were restricted to the CON and 3%Thy groups, with the 3%Thy group selected after evaluation of the phenotypic and physiological responses rather than being prespecified before the experiment. These omics comparisons were therefore considered exploratory. No statistically significant difference in overall fecal microbial community composition was detected between the two groups, whereas 17 candidate differential fecal metabolite features were observed. These features were mainly putatively annotated as lipids and lipid-like molecules, organic acids and derivatives, organoheterocyclic compounds, and benzenoids. Among the supplementation levels evaluated, the 3%Thy group showed comparatively favorable numerical responses in several measured parameters and may be considered a candidate level for further investigation. However, the present findings do not establish an optimal thymol supplementation level, and the biological significance of the exploratory fecal omics results requires further validation. FAU - Ma, Chen AU - Ma C AD - College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China. FAU - Li, Chao AU - Li C AD - Apiculture Technology Development Center of Xinjiang Uygur Autonomous Region, Urumqi 830001, China. FAU - Li, Haiying AU - Li H AD - College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China. FAU - Liu, Jiajia AU - Liu J AD - Institute of Animal Husbandry, Xinjiang Academy of Animal Husbandry, Urumqi 830011, China. FAU - Liao, Kunyu AU - Liao K AD - College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China. FAU - Li, Yuanhao AU - Li Y AD - College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China. FAU - Ren, Jie AU - Ren J AD - College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China. FAU - Wei, Guoqiang AU - Wei G AD - College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China. FAU - Li, Xiaobin AU - Li X AD - College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China. LA - eng GR - 2026650005000075/The Horizontal Research Project of Xinjiang Agricultural University/ GR - 2023B02036-2, 2023B02036-2-1, and 2023B02036-2-2/The Key Research and Development Program of the Xinjiang Uygur Autonomous Region/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700596 OTO - NOTNLM OT - antioxidant capacity OT - fecal metabolites OT - health care sand OT - squabs OT - thymol COIS- Author Jiajia Liu was employed by Xinjiang Hetian Lanhai Pigeon Industry Development Co., Ltd., China. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:32 PHST- 2026/09/22 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ani16203314 [pii] AID - animals-16-03314 [pii] AID - 10.3390/ani16203314 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 21;16(20):3314. doi: 10.3390/ani16203314. PMID- 42939239 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 21 TI - Estimation of Genomic Breeding Values for Economic Traits in Alxa-Type Cashmere Goats Using Bayesian Methods. LID - 10.3390/ani16203308 [doi] LID - 3308 AB - Conventional breeding has a long generation interval; genomic selection can enable early selection and shorten the breeding process in cashmere goats. This study aimed to estimate genomic breeding values for economic traits in Inner Mongolia (Alxa-type) cashmere goats using Bayesian methods. Whole-genome resequencing at 1x depth was performed on 491 Alxa-type cashmere goats (40 males and 451 females), and genotypes were imputed to 10x using a reference panel with an average imputation accuracy of 97.90%. Fixed effects were determined by analysis of variance, and breeding values for body weight, cashmere fiber diameter, and cashmere yield were estimated using Bayes A, Bayes B, and Bayes C; prediction accuracy and unbiasedness were evaluated by five-fold cross-validation. Measurement year and group affected all three traits highly significantly (p < 0.01); sex affected body weight and cashmere yield highly significantly (p < 0.01) but no significant effect on cashmere fiber diameter (p > 0.05); and maternal age had a significant effect on body weight (p < 0.05), but no significant effect on cashmere fiber diameter or cashmere yield (p > 0.05); group had a highly significant effect on all three traits (p < 0.01). Breeding values were estimated using Bayes A, Bayes B, and Bayes C, with five-fold cross-validation, and the three methods performed comparably overall in this population. FAU - Quan, Jie AU - Quan J AD - Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China. FAU - Wu, Tiecheng AU - Wu T AD - Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China. AD - National and Local Joint Engineering Research Center for Genetic Resources Evaluation and Breeding Technology of Meat Sheep (Inner Mongolia), Hohhot 010031, China. FAU - Wang, Tao AU - Wang T AD - Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China. FAU - Liu, Junyang AU - Liu J AUID- ORCID: 0000-0001-7303-9297 AD - Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China. FAU - Guo, Mengyu AU - Guo M AD - Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China. FAU - Yi, Leqi AU - Yi L AD - Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China. FAU - Liu, Bin AU - Liu B AD - Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China. LA - eng GR - No. 2026YFHH0193/Science and Technology Planning Project of Inner Mongolia Autonomous Region/ GR - No. JBYWF2025-YFGG06/Basic Research Operating Expenses Program/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700610 OTO - NOTNLM OT - Bayesian methods OT - cashmere goat OT - economic traits OT - genomic selection COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/21 CRDT- 2026/10/27 10:32 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - ani16203308 [pii] AID - animals-16-03308 [pii] AID - 10.3390/ani16203308 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 21;16(20):3308. doi: 10.3390/ani16203308. PMID- 42939233 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 20 TI - Genome-Wide Association and Selection Signature Analyses of Hematological and Serum Biochemical Traits in Yunling Cattle. LID - 10.3390/ani16203302 [doi] LID - 3302 AB - Yunling cattle, a native breed native to subtropical regions, exhibit remarkable traits such as heat tolerance, strong immunity, and tick resistance. To characterize the genetic architecture of blood physiological and biochemical traits associated with these adaptations, we performed a genome-wide association study (GWAS) using whole-genome resequencing data from 97 individuals combined alongside 39 blood traits (30 analysed after quality control). Significant association signals were detected for seven traits: albumin (ALB), homocysteine (CYS), white blood cell count (WBC), bilirubin (BIL), indirect bilirubin (I-BIL), low-density lipoprotein cholesterol (LD-CHO), and red cell distribution width (RDW). Notably, loci related to leukocyte parameters overlapped with strong selection signals and were mapped to classical bovine leukocyte antigen (BOLA) genes, including BOLA, BOLA-DQB and BOLA-DQA5, which are located within the MHC region and are known to be involved in immune regulation. For lipid-related traits, the strongest signals were identified near RPL22L1 and SLC7A14 on chromosome 1, while additional associated genes included XPOT, SLC16A7, SCAI, SYT14 and ATP2B2. Additionally, regarding metabolic and protein parameters, NHSL1 showed a strong association with bilirubin; this gene has previously been reported to be associated with hypertension. Red blood cell parameters revealed candidate genes such as NAV3, LSAMP, ACACA, and others linked to erythrocyte variation. For Homocysteine-related traits, CIITA was significantly associated with Homocysteine levels; CIITA is known to be involved in immune regulation. In conclusion, this study provides a genome-wide characterization of the genetic architecture of blood traits in Yunling cattle, identifying several candidate genes (e.g., MOB1B, CIITA and BOLA genes) associated with blood physiological and biochemical traits. These candidate loci provide a foundation for further functional validation; validation in larger and independent populations is needed before any application. FAU - Shen, Jiafei AU - Shen J AD - Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-Agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng 224051, China. FAU - Wang, Yanan AU - Wang Y AD - Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-Agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng 224051, China. FAU - Dong, Yunxin AU - Dong Y AD - Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-Agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng 224051, China. FAU - Lv, Qianlin AU - Lv Q AD - Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-Agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng 224051, China. FAU - Huang, Yiming AU - Huang Y AD - Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-Agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng 224051, China. FAU - Miao, Yingcan AU - Miao Y AD - Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-Agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng 224051, China. FAU - Hanif, Quratulain AU - Hanif Q AUID- ORCID: 0000-0002-9841-8055 AD - Department of Life Sciences, School of Science, University of Management and Technology, Lahore 54770, Pakistan. FAU - Qu, Kaixing AU - Qu K AD - Academy of Science and Technology, Chuxiong Normal College, Chuxiong 675000, China. FAU - Lei, Chuzhao AU - Lei C AUID- ORCID: 0000-0003-1647-1037 AD - Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. LA - eng PT - Journal Article DEP - 20261020 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700815 OTO - NOTNLM OT - GWAS OT - Yunling cattle OT - blood traits OT - candidate genes OT - selection signature COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:32 PHST- 2026/08/24 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - ani16203302 [pii] AID - animals-16-03302 [pii] AID - 10.3390/ani16203302 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 20;16(20):3302. doi: 10.3390/ani16203302. PMID- 42939232 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 20 TI - Deep Learning Models for FAMACHA(c)-Based Anemia Screening in Goats. LID - 10.3390/ani16203301 [doi] LID - 3301 AB - Haemonchus contortus infection is a major cause of anemia in goats and can reduce productivity, reproductive performance, and animal welfare. The FAMACHA(c) system supports on-farm anemia screening by visually assessing ocular conjunctival color, but scoring can vary with evaluator experience, and lighting conditions. This study developed a smartphone-oriented deep learning framework for FAMACHA(c)-based anemia screening in goats using ocular conjunctival images. The dataset included 1995 FAMACHA(c)-labeled images from 141 meat and dairy goats collected across three acquisition campaigns. For the binary screening task, FAMACHA(c) scores of 4-5 were assigned to the anemic screening class, whereas scores of 1-3 were assigned to the non-anemic screening class. The pipeline combined U-Net-based conjunctiva segmentation with classification using six ImageNet-pretrained backbones: ResNet-50, ViT-B/16, Swin-T, EfficientNet-B0, MobileNetV4-Small, and MobileViT-v2-1.0. Models were evaluated using animal-level dataset splitting, nested cross-validation, validation-selected thresholds, goat-level cluster bootstrap confidence intervals and goat-block paired permutation tests. U-Net with a ResNet-34 encoder achieved a foreground IoU of 0.7342 and a Dice coefficient of 0.8372 for conjunctiva segmentation. On the held-out test set, ViT-B/16 achieved the highest anemia recall (0.924), accuracy (0.861), and anemia-class F1-score (0.879). However, MobileNetV4-Small performed closely, with 0.847 accuracy, a 0.861 F1-score, and the highest specificity (0.825). U-Net-ResNet34 combined with MobileNetV4-Small yielded the lowest estimated combined inference latency of 479.04 ms per image, calculated from separately measured segmentation and classification latencies on a Samsung SM-A556E smartphone using TensorFlow Lite. A Pearson's correlation analysis showed a strong positive association between fecal egg count and FAMACHA(c) score (r = 0.7082, R(2) = 0.5015, p < 0.0001). These results suggest that ocular-conjunctival imaging can support FAMACHA(c)-based anemia screening in goats, with ViT-B/16 serving as the strongest recall-oriented model and MobileNetV4-Small as a practical candidate for low-latency on-device inference under the tested smartphone configuration. FAU - Hilaire, Mariline AU - Hilaire M AD - Department of Agricultural and Environmental Sciences, Tuskegee University, Tuskegee, AL 360088, USA. FAU - Bramantyo, Hutama Arif AU - Bramantyo HA AD - Department of Electrical and Computer Engineering, Auburn University, Auburn, AL 36849, USA. FAU - Chamoun, Sam AU - Chamoun S AD - Department of Electrical and Computer Engineering, Auburn University, Auburn, AL 36849, USA. FAU - Sun, Yin AU - Sun Y AUID- ORCID: 0000-0001-6811-984X AD - Department of Electrical and Computer Engineering, Auburn University, Auburn, AL 36849, USA. FAU - Wang, Honghe AU - Wang H AD - Department of Biology, Tuskegee University, Tuskegee, AL 36849, USA. FAU - Chaudhary, Santosh AU - Chaudhary S AD - Department of Agricultural and Environmental Sciences, Tuskegee University, Tuskegee, AL 360088, USA. FAU - Kanyi, Vivian AU - Kanyi V AD - Department of Agricultural and Environmental Sciences, Tuskegee University, Tuskegee, AL 360088, USA. FAU - Ismael, Hossam AU - Ismael H AUID- ORCID: 0000-0002-1882-9760 AD - Department of Agricultural and Environmental Sciences, Tuskegee University, Tuskegee, AL 360088, USA. FAU - Abdo, Heba AU - Abdo H AD - Department of Agricultural and Environmental Sciences, Tuskegee University, Tuskegee, AL 360088, USA. FAU - Min, Byeng Ryel AU - Min BR AUID- ORCID: 0000-0002-4334-8336 AD - Department of Agricultural and Environmental Sciences, Tuskegee University, Tuskegee, AL 360088, USA. LA - eng GR - NR233A7500004G103/National Institute of Food and Agriculture/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700711 OTO - NOTNLM OT - FAMACHA(c), goat anemia OT - deep learning OT - image segmentation OT - ocular conjunctiva OT - vision transformer COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/20 CRDT- 2026/10/27 10:32 PHST- 2026/08/23 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - ani16203301 [pii] AID - animals-16-03301 [pii] AID - 10.3390/ani16203301 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 20;16(20):3301. doi: 10.3390/ani16203301. PMID- 42939225 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 19 TI - Integrating Camera-Trap Monitoring and Community Surveys to Assess Human-Asian Badger (Meles leucurus) Interactions in Agricultural Landscapes of the Meng Mountains, China. LID - 10.3390/ani16203294 [doi] LID - 3294 AB - Human-wildlife interactions can threaten rural livelihoods and complicate wildlife conservation in agricultural landscapes. We combined community surveys and camera-trap monitoring as complementary sources of information to assess human-Asian badger (Meles leucurus) interactions across four sites in the Meng Mountains, China. We surveyed 400 residents and monitored 82 camera-trap stations from April 2024 to August 2026, representing 72,406 trap-nights. Reported encounter frequency differed significantly among study sites (chi(2) = 139.36, p < 0.001), with evening and autumn most frequently reported. Agricultural land ownership was associated with reported crop damage (chi(2) = 16.64, p = 0.0002), while livestock ownership was associated with both reported crop damage (chi(2) = 36.23, p < 0.001) and conflict awareness (chi(2) = 60.47, p < 0.001). Camera traps recorded 103 independent detections at 52 of 82 targeted stations (detection proportion = 0.63). Because stations were selected where badger occurrence was suspected, this proportion should not be interpreted as landscape-wide occupancy. Detections differed among diurnal, crepuscular, and nocturnal periods (chi(2) = 14.99, p < 0.001), with greater representation during diurnal and crepuscular periods. Together, the ecological and questionnaire data provide complementary evidence on badger occurrence, agricultural exposure, livelihood activities, and human perceptions relevant to reported human-badger conflict. Integrating ecological monitoring with community information may help inform targeted, non-lethal conflict mitigation and coexistence planning. FAU - Khan, Kamal Ahmed AU - Khan KA AUID- ORCID: 0000-0001-8403-2322 AD - College of Life Sciences, Shandong Normal University, Jinan 250014, China. FAU - Liu, Tengteng AU - Liu T AUID- ORCID: 0000-0002-0182-2562 AD - College of Life Sciences, Shandong Normal University, Jinan 250014, China. FAU - Gul, Zartasha AU - Gul Z AD - Department of Zoology, Government Girls' Degree College, Khalabat Township, Haripur 22620, Pakistan. FAU - Shi, Shaofei AU - Shi S AD - Mengyin County State-Owned Forest Farm, Linyi 276000, China. FAU - Shao, Changshan AU - Shao C AD - Yimengshan World Geopark Administration, Mengshan Conservation and Development Center, Linyi 276000, China. FAU - Gong, Jianhua AU - Gong J AD - Mengyin County State-Owned Forest Farm, Linyi 276000, China. FAU - Shi, Zenglei AU - Shi Z AD - Mengyin County State-Owned Forest Farm, Linyi 276000, China. FAU - Yang, Guiwen AU - Yang G AD - College of Life Sciences, Shandong Normal University, Jinan 250014, China. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700675 OTO - NOTNLM OT - coexistence OT - diel activity OT - human-wildlife conflict OT - mesocarnivore OT - public perception OT - reported crop depredation OT - rural livelihoods COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:32 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ani16203294 [pii] AID - animals-16-03294 [pii] AID - 10.3390/ani16203294 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 19;16(20):3294. doi: 10.3390/ani16203294. PMID- 42939221 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 19 TI - Conjoined Twins in Guinea Pigs (Cavia porcellus): Case Report. LID - 10.3390/ani16203291 [doi] LID - 3291 AB - This study reports a case of conjoined twins in guinea pigs (Cavia porcellus) from the Peru breed, in the Cajamarca region, Peru. A one-year-old female gave birth to normal offspring and a pair of conjoined twins born dead; The latter were carried out with morphological, radiographic and echographic evaluations, and the blood count and serum biochemical profile of the dam were analyzed. The twins presented cephalothoraco-omphalopagus fusion, characterized by a single skull with cyclopia, arhinia and astomy, ventral thoracoabdominal fusion, the pelvis separated and four extremities (four pairs) completely dislocated. Radiographic and ultrasound evaluations identified two hearts and two pairs of kidneys, with preserved gross sonographic morphology and no evident major structural abnormalities. The maternal blood count showed mild hypochromic macrocytosis and mild thrombocytopenia, with the rest of the hematological and biochemical parameters within normal ranges. It was concluded that the hallmarks are compatible with an early developmental disturbance affecting midline structures, although the precise timing and etiology could not be definitively established, which selectively affected the structures of the medial line. This report expands the scarcity of evidence available on serious congenital malformations in guinea pigs. FAU - Cueva-Rodriguez, Medali AU - Cueva-Rodriguez M AUID- ORCID: 0000-0002-1301-5477 AD - Veterinary Pathology Laboratory, Faculty of Veterinary Sciences, National University of Cajamarca, Cajamarca, Av. Atahualpa, Km. 3, Cajamarca 06003, Peru. FAU - Sanchez-Estela, Jhenny AU - Sanchez-Estela J AUID- ORCID: 0009-0006-4132-9373 AD - Veterinary Pathology Laboratory, Faculty of Veterinary Sciences, National University of Cajamarca, Cajamarca, Av. Atahualpa, Km. 3, Cajamarca 06003, Peru. FAU - Portal-Torres, Jorge AU - Portal-Torres J AD - Veterinary Pathology Laboratory, Faculty of Veterinary Sciences, National University of Cajamarca, Cajamarca, Av. Atahualpa, Km. 3, Cajamarca 06003, Peru. FAU - Reyna-Cotrina, Giussepe AU - Reyna-Cotrina G AD - Veterinary Pathology Laboratory, Faculty of Veterinary Sciences, National University of Cajamarca, Cajamarca, Av. Atahualpa, Km. 3, Cajamarca 06003, Peru. FAU - Mejia, Flor L AU - Mejia FL AUID- ORCID: 0000-0002-1851-1285 AD - Agrostology Laboratory, Institute for Livestock and Biotechnology Research, Faculty of Animal Science, Biotechnology, Agribusiness and Data Science, Toribio Rodriguez de Mendoza National University of Amazonas, Chachapoyas 01001, Peru. FAU - Castillo-Santamaria, Bessy AU - Castillo-Santamaria B AD - Chemistry Laboratory, Faculty of Agricultural Sciences, National University of Canete, San Vicente de Canete 15701, Peru. FAU - Estela-Manrique, Judith AU - Estela-Manrique J AUID- ORCID: 0009-0001-6484-550X AD - National Institute of Agricultural Innovation, Banos del Inca Agricultural Research Station, Cajamarca, Jiron Wiracocha, No. S/N, Los Banos del Inca 06004, Peru. FAU - Bardales Escalante, William AU - Bardales Escalante W AUID- ORCID: 0000-0001-9721-9057 AD - Institute for Research in Livestock and Biotechnology, Faculty of Animal Science, Biotechnology, Agribusiness and Data Science, Toribio Rodriguez de Mendoza National University of Amazonas, Chachapoyas 01001, Peru. LA - eng PT - Case Reports PT - Journal Article DEP - 20261019 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700570 OTO - NOTNLM OT - cephalopagus OT - congenital malformation OT - guinea pig OT - omphalopagus OT - teratology OT - thoracopagus COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/19 CRDT- 2026/10/27 10:32 PHST- 2026/08/13 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - ani16203291 [pii] AID - animals-16-03291 [pii] AID - 10.3390/ani16203291 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 19;16(20):3291. doi: 10.3390/ani16203291. PMID- 42939216 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 18 TI - Mitochondrial Dysfunction Across Multiple Organs in Broilers Exhibiting Wooden Breast Myopathy. LID - 10.3390/ani16203285 [doi] LID - 3285 AB - Wooden breast (WB) in broilers has been linked to impaired mitochondrial function in the breast muscle. When mitochondrial function declines, organs receive less ATP and experience oxidative stress. Muscle development is linked to the optimal function of the gut, liver, and pancreas, which depends on adequate ATP to support nutrient absorption and metabolism. The effects of the WB myopathy on other organs and tissues within broilers are relatively unknown. The aim of this study was to investigate factors linked to mitochondrial function in multiple organs from broilers exhibiting WB. From sixty 8-week-old broilers, ten severe WB and ten normal breast muscles were selected for tissue collection (liver, pancreas, three sections of intestine, and breast muscle). Reduced citrate synthase activity and mitochondrial complex-II activity were observed in the muscle, pancreas, liver and duodenum tissues of WB broilers compared to normal broilers. Broilers with WB had reduced glutathione content in the pancreas and all three sections of the intestine when compared to normal broilers. Whole tissue ATP content was decreased in the muscle and liver of WB broilers. Overall, these data suggest that mitochondrial function may be impaired in tissues beyond just the breast muscle in broilers that exhibit the WB myopathy. FAU - Shakeri, Majid AU - Shakeri M AUID- ORCID: 0000-0002-1857-2133 AD - Oak Ridge Institute for Science and Education, Oak Ridge, TN 37830, USA. AD - USDA-ARS, U.S. National Poultry Research Center, Athens, GA 30605, USA. FAU - Chatterjee, Debolina AU - Chatterjee D AD - USDA-ARS, U.S. National Poultry Research Center, Athens, GA 30605, USA. FAU - Rafieian-Naeini, Hamid Reza AU - Rafieian-Naeini HR AD - Department of Poultry Science, University of Georgia, Athens, GA 30602, USA. FAU - Kim, Woo Kyun AU - Kim WK AUID- ORCID: 0000-0002-0005-352X AD - Department of Poultry Science, University of Georgia, Athens, GA 30602, USA. FAU - Kong, Byungwhi AU - Kong B AD - USDA-ARS, U.S. National Poultry Research Center, Athens, GA 30605, USA. FAU - Bowker, Brian AU - Bowker B AD - USDA-ARS, U.S. National Poultry Research Center, Athens, GA 30605, USA. LA - eng GR - No. DE-SC0014664/United States Department of Energy/ GR - DE-SC0014664/Oak Ridge Associated Universities/ PT - Journal Article DEP - 20261018 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700773 OTO - NOTNLM OT - breast myopathy OT - broiler OT - meat quality OT - mitochondrial function OT - wooden breast COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:32 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - ani16203285 [pii] AID - animals-16-03285 [pii] AID - 10.3390/ani16203285 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 18;16(20):3285. doi: 10.3390/ani16203285. PMID- 42939215 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 18 TI - An Enhanced Genomic Transformer for Multi-Dataset Forward-Validation Benchmarking in Pig Genomic Prediction. LID - 10.3390/ani16203284 [doi] LID - 3284 AB - Genomic prediction models in pig breeding should forecast the performance of future selection candidates, yet random cross-validation does not accurately reflect this sequential setting. Here, we introduce an enhanced genomic transformer (EGT) that compresses genome-wide SNP profiles into compact tokens and incorporates a differentiable scoring term alongside mean squared error (MSE) during training. We constructed 70%/10%/20% forward-validation datasets for three pig datasets and evaluated single-trait and multi-task EGT variants against GBLUP, support-vector regression, random forest, XGBoost, CNN, and RNN across 12 prediction tasks. The better-performing EGT variant achieved a higher test-set Pearson correlation coefficient (PCC) than the baseline for 10 of the 12 prediction tasks, with the largest improvement on LDL-C (+0.057). In BloodLipid ablations, autoencoder-only representation yielded the highest mean PCC (0.285), while Pearson-MSE objective reached 0.293 versus 0.291 for MSE-only training. Integrated-gradient analysis revealed a recurrent candidate region on Sus scrofa chromosome 13 across three lipid traits, and masking the top-attributed features reduced predictive performance more than a marker-count-matched random control for HDL-C, LDL-C and TG, whereas masking increased test-set PCC for TCHOL. This work establishes forward-validation benchmarks across three pig datasets and reports results to inform model selection and region prioritization in pig breeding. FAU - Xiang, Weixi AU - Xiang W AD - Agricultural Information Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China. AD - Key Laboratory of Agricultural Big Data, Ministry of Agriculture and Rural Affairs, Beijing 100081, China. FAU - Li, Zhaoxin AU - Li Z AUID- ORCID: 0000-0001-5450-8131 AD - Agricultural Information Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China. AD - Key Laboratory of Agricultural Big Data, Ministry of Agriculture and Rural Affairs, Beijing 100081, China. FAU - Sun, Qixin AU - Sun Q AUID- ORCID: 0000-0001-6231-2227 AD - Agricultural Information Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China. AD - Key Laboratory of Agricultural Big Data, Ministry of Agriculture and Rural Affairs, Beijing 100081, China. FAU - Chai, Xiujuan AU - Chai X AUID- ORCID: 0000-0002-2757-9900 AD - Agricultural Information Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China. AD - Key Laboratory of Agricultural Big Data, Ministry of Agriculture and Rural Affairs, Beijing 100081, China. FAU - Sun, Tan AU - Sun T AUID- ORCID: 0000-0002-8257-5064 AD - Agricultural Information Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China. AD - Key Laboratory of Agricultural Big Data, Ministry of Agriculture and Rural Affairs, Beijing 100081, China. LA - eng GR - Ministry of Agriculture and Rural Affairs/ PT - Journal Article DEP - 20261018 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700791 OTO - NOTNLM OT - forward validation OT - genomic prediction OT - multi-task learning OT - pig breeding OT - transformer COIS- The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:32 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - ani16203284 [pii] AID - animals-16-03284 [pii] AID - 10.3390/ani16203284 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 18;16(20):3284. doi: 10.3390/ani16203284. PMID- 42939214 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 18 TI - Antimicrobial Resistance Profiles of Staphylococcus spp. Isolated from the Oral Cavity of Captive Wild Mammals at a Zoo in Southern Brazil. LID - 10.3390/ani16203283 [doi] LID - 3283 AB - Antimicrobial resistance in Staphylococcus spp. poses a challenge to One Health due to the potential for transmission among humans, animals, and the environment. This study investigated antimicrobial resistance patterns in Staphylococcus spp. isolates from the oral cavities of non-human primates and wild felids kept in captivity at a zoo in southern Brazil. Samples were collected from Sapajus apella and wild felids (Leopardus pardalis, Leopardus guttulus, Herpailurus yagouaroundi, Leopardus wiedii, Panthera tigris, Puma concolor, Panthera onca). Bacterial isolation and identification were performed using phenotypic methods, followed by antimicrobial susceptibility testing and molecular diagnosis for the detection of Staphylococcus aureus and the mecA gene. Of the Staphylococcus spp. isolates, 64.5% originated from non-human primates and 35.5% from wild felids. S. aureus was identified in 12.9% of the isolates, all from non-human primates. Azithromycin, erythromycin, and sulfamethoxazole-trimethoprim showed 90.3% resistance. Additionally, 80.6% of the isolates were multidrug-resistant (MDR), and 90.3% demonstrated a multiple antibiotic resistance (MAR) index, suggesting possible exposure to antimicrobial selective pressure. These findings demonstrate the circulation of multidrug-resistant Staphylococcus spp. in captive animals and reinforce the importance of antimicrobial resistance surveillance from a One Health perspective. FAU - Bonato, Francieli Gesleine Capote AU - Bonato FGC AUID- ORCID: 0000-0003-3235-5124 AD - Programa de Pos-Graduacao em Ciencia Animal com Enfase em Produtos Bioativos, Universidade Paranaense, Umuarama 87502-210, Parana, Brazil. FAU - Andrade, Ana Claudia Souza AU - Andrade ACS AD - Programa de Pos-Graduacao em Ciencia Animal com Enfase em Produtos Bioativos, Universidade Paranaense, Umuarama 87502-210, Parana, Brazil. FAU - Tanaka, Henrique Susumu AU - Tanaka HS AD - Programa de Pos-Graduacao em Ciencia Animal com Enfase em Produtos Bioativos, Universidade Paranaense, Umuarama 87502-210, Parana, Brazil. FAU - Azevedo, Jorge Fernandes de AU - Azevedo JF AD - Programa de Pos-Graduacao em Ciencia Animal com Enfase em Produtos Bioativos, Universidade Paranaense, Umuarama 87502-210, Parana, Brazil. FAU - Rubio, Kariny Aparecida Jardim AU - Rubio KAJ AUID- ORCID: 0000-0002-5243-2986 AD - Programa de Pos-Graduacao em Ciencia Animal com Enfase em Produtos Bioativos, Universidade Paranaense, Umuarama 87502-210, Parana, Brazil. FAU - Oliveira, Halison Murilo da Silva AU - Oliveira HMDS AD - Programa de Pos-Graduacao em Ciencia Animal com Enfase em Produtos Bioativos, Universidade Paranaense, Umuarama 87502-210, Parana, Brazil. FAU - Marchi, Heloiza de Oliveira AU - Marchi HO AD - Curso de Medicina Veterinaria, Universidade Paranaense, Umuarama 87502-210, Parana, Brazil. FAU - Takahashi, Vitor Yukio AU - Takahashi VY AUID- ORCID: 0009-0002-7493-0350 AD - Curso de Medicina Veterinaria, Universidade Paranaense, Umuarama 87502-210, Parana, Brazil. FAU - Silva, Bruna Pereira Bitencourt da AU - Silva BPBD AD - Zoologico Municipal, Curitiba 81860-270, Parana, Brazil. FAU - Ribeiro, Rodrigo Neca AU - Ribeiro RN AD - Zoologico Municipal, Curitiba 81860-270, Parana, Brazil. FAU - Secundini, Wesley Alves AU - Secundini WA AD - Mestrado Profissional de Plantas Medicinais e Fitoterapicos na Atencao Basica, Universidade Paranaense, Umuarama 87502-210, Parana, Brazil. FAU - Silva, Gabriela Catuzo Canonico AU - Silva GCC AUID- ORCID: 0000-0002-4123-9507 AD - Programa de Pos-Graduacao em Biotecnologia Aplicada a Agricultura, Universidade Paranaense, Umuarama 87502-210, Parana, Brazil. FAU - Bonato, Denis Vinicius AU - Bonato DV AD - Programa de Pos-Graduacao em Ciencia Animal com Enfase em Produtos Bioativos, Universidade Paranaense, Umuarama 87502-210, Parana, Brazil. FAU - Piau Junior, Ranulfo AU - Piau Junior R AUID- ORCID: 0000-0003-4765-6544 AD - Programa de Pos-Graduacao em Ciencia Animal com Enfase em Produtos Bioativos, Universidade Paranaense, Umuarama 87502-210, Parana, Brazil. FAU - Gazim, Zilda Cristiani AU - Gazim ZC AUID- ORCID: 0000-0003-0392-5976 AD - Programa de Pos-Graduacao em Ciencia Animal com Enfase em Produtos Bioativos, Universidade Paranaense, Umuarama 87502-210, Parana, Brazil. AD - Mestrado Profissional de Plantas Medicinais e Fitoterapicos na Atencao Basica, Universidade Paranaense, Umuarama 87502-210, Parana, Brazil. FAU - Barbosa, Lidiane Nunes AU - Barbosa LN AUID- ORCID: 0000-0001-5762-8091 AD - Programa de Pos-Graduacao em Ciencia Animal com Enfase em Produtos Bioativos, Universidade Paranaense, Umuarama 87502-210, Parana, Brazil. AD - Mestrado Profissional de Plantas Medicinais e Fitoterapicos na Atencao Basica, Universidade Paranaense, Umuarama 87502-210, Parana, Brazil. FAU - Goncalves, Daniela Dib AU - Goncalves DD AUID- ORCID: 0000-0001-8322-8905 AD - Programa de Pos-Graduacao em Ciencia Animal com Enfase em Produtos Bioativos, Universidade Paranaense, Umuarama 87502-210, Parana, Brazil. AD - Mestrado Profissional de Plantas Medicinais e Fitoterapicos na Atencao Basica, Universidade Paranaense, Umuarama 87502-210, Parana, Brazil. LA - eng GR - Call PQ 2023 - 310953/2023-5/National Council for Scientific and Technological Development/ PT - Journal Article DEP - 20261018 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700745 OTO - NOTNLM OT - One Health OT - antimicrobial susceptibility OT - captive animals OT - multidrug-resistant bacteria OT - wild mammals COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/18 CRDT- 2026/10/27 10:32 PHST- 2026/09/18 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - ani16203283 [pii] AID - animals-16-03283 [pii] AID - 10.3390/ani16203283 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 18;16(20):3283. doi: 10.3390/ani16203283. PMID- 42939208 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 17 TI - Sleep and Drowsiness in Cattle: A Narrative Review of Physiology, Measurement, and Methodological Challenges. LID - 10.3390/ani16203279 [doi] LID - 3279 AB - Sleep in cattle is a complex physiological state comprising non-rapid eye movement (NREM) and rapid eye movement (REM) sleep, while drowsing represents an intermediate vigilance state between wakefulness and sleep. Research has progressed from invasive electrophysiological recordings to noninvasive polysomnography (PSG), behavioral assessment, accelerometry, and automated classification approaches. However, accurately identifying and quantifying vigilance states under production conditions remains challenging. This narrative review, based on 22 primary studies identified through searches of Web of Science, Scopus and Google Scholar, critically synthesizes current knowledge of sleep physiology in cattle, the approaches used to assess it, and the principal methodological limitations affecting interpretation of the available evidence. Cattle exhibit a polyphasic sleep pattern, with NREM generally accounting for the largest proportion of total sleep. However, reported sleep and drowsing durations vary considerably among studies, and these differences may reflect both biological variation and methodological factors. Drowsing is particularly difficult to characterize because its behavioral and physiological features overlap with quiet wakefulness and light sleep. Rumination represents an additional challenge because associated muscular activity can interfere with electrophysiological recordings and complicate vigilance-state classification. Behavioral observations and accelerometry provide scalable measures of resting and sleep-like behavior but cannot independently establish physiological sleep, whereas PSG provides greater physiological resolution while remaining susceptible to artifacts and practical constraints. Current evidence is insufficient to establish causal relationships between sleep variation and cattle health, welfare, or productive performance. Future research should improve physiological classification during overlapping behavioral states, better validate scalable monitoring approaches, and evaluate the functional consequences of sleep variation across diverse cattle populations and production conditions. FAU - Torres, Pedro H F AU - Torres PHF AD - School of Agricultural and Veterinary Sciences, Sao Paulo State University (UNESP), Jaboticabal 14884-900, SP, Brazil. FAU - Alves, Fernanda F AU - Alves FF AUID- ORCID: 0000-0003-3442-0532 AD - School of Agricultural and Veterinary Sciences, Sao Paulo State University (UNESP), Jaboticabal 14884-900, SP, Brazil. FAU - Lopes, Maria Eduarda M AU - Lopes MEM AUID- ORCID: 0009-0004-5857-547X AD - School of Agricultural and Veterinary Sciences, Sao Paulo State University (UNESP), Jaboticabal 14884-900, SP, Brazil. FAU - Virginio Junior, Gercino F AU - Virginio Junior GF AUID- ORCID: 0000-0001-7437-138X AD - School of Agricultural and Veterinary Sciences, Sao Paulo State University (UNESP), Jaboticabal 14884-900, SP, Brazil. FAU - O'Hara, Bruce F AU - O'Hara BF AUID- ORCID: 0000-0002-5553-8209 AD - Department of Biology, University of Kentucky, Lexington, KY 40508-0225, USA. FAU - Donohue, Kevin D AU - Donohue KD AUID- ORCID: 0000-0003-4423-6296 AD - Department of Electrical and Computer Engineering, University of Kentucky, Lexington, KY 40508-0225, USA. FAU - Watanabe, Daniel H M AU - Watanabe DHM AUID- ORCID: 0009-0001-2166-6149 AD - School of Agricultural and Veterinary Sciences, Sao Paulo State University (UNESP), Jaboticabal 14884-900, SP, Brazil. FAU - Millen, Danilo D AU - Millen DD AUID- ORCID: 0000-0002-1253-7310 AD - School of Agricultural and Veterinary Sciences, Sao Paulo State University (UNESP), Jaboticabal 14884-900, SP, Brazil. LA - eng GR - 2022/16247-4/Sao Paulo Research Foundation/ PT - Journal Article PT - Review DEP - 20261017 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700617 OTO - NOTNLM OT - accelerometry OT - cattle OT - drowsing OT - polysomnography OT - rumination OT - sleep COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/17 CRDT- 2026/10/27 10:32 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - ani16203279 [pii] AID - animals-16-03279 [pii] AID - 10.3390/ani16203279 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 17;16(20):3279. doi: 10.3390/ani16203279. PMID- 42939201 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 17 TI - Allele and Genotype Frequencies of Production Trait SNPs in Crossbred Heifers of the Colombian Caribbean. LID - 10.3390/ani16203270 [doi] LID - 3270 AB - The Colombian Caribbean dual-purpose livestock system (Bos taurus x Bos indicus) is an important source of milk and beef, yet molecular characterization of trait-associated markers remains limited. This study characterized allele and genotype frequencies of 29 single-nucleotide polymorphisms (SNPs) associated with meat quality and milk production traits in 1585 crossbred heifers from five departments of the Colombian Caribbean. DNA was extracted from hair follicles and genotyped with the Axiom Bovine 100K Array. Markers spanned genes for muscle growth and proteolysis (CAPN1, CAST, MSTN), energy metabolism (LEP, DGAT1), and milk protein composition (CSN2, CSN3, PAEP, GH1, GHR, ABCG2). Hardy-Weinberg equilibrium (HWE) was assessed by chi-squared test. Of the 29 markers, 21 were polymorphic and testable: 19 conformed to HWE and 2 deviated (PAEP_239, PAEP_312). The remaining 8 were monomorphic. Approximately 48% of loci showed very high favorable allele frequencies (q > 0.99 or p > 0.99), whereas 52% maintained intermediate heterozygosity. CSN3_526 reached near-fixation of the favorable B allele (q = 0.996), consistent with selection for cheese yield, whereas CAST_282 and CAST_2870 showed high heterozygosity (f(AB) approximately 0.47-0.48), indicating substantial remaining variation at these loci. These findings provide the first descriptive dual meat-and-milk SNP frequency profile for these crossbred heifers and a molecular baseline for the future development of bioeconomic selection indices in tropical dual-purpose systems. Frequencies are reported at the pooled and department-stratified levels; the results are descriptive and require phenotypic validation before any inference on marker effects or suitability for selection. FAU - Ahumada Velasco, Marcos AU - Ahumada Velasco M AUID- ORCID: 0000-0003-4302-4689 AD - CTIE (Centro de Transferencia, Innovacion y Emprendimiento of the Universidad de Cartagena), Universidad de Cartagena, Cartagena de Indias 130001, Colombia. FAU - Rodriguez Serrano, Andres Camilo AU - Rodriguez Serrano AC AUID- ORCID: 0000-0002-8243-2266 AD - CTIE (Centro de Transferencia, Innovacion y Emprendimiento of the Universidad de Cartagena), Universidad de Cartagena, Cartagena de Indias 130001, Colombia. FAU - Corrales Alvarez, Juan David AU - Corrales Alvarez JD AUID- ORCID: 0000-0001-5994-4637 AD - Faculty of Agricultural Sciences, La Salle University, Bogota 111321, Colombia. FAU - Cardenas Beltran, Jesus Maria AU - Cardenas Beltran JM AUID- ORCID: 0000-0002-8381-3044 AD - CTIE (Centro de Transferencia, Innovacion y Emprendimiento of the Universidad de Cartagena), Universidad de Cartagena, Cartagena de Indias 130001, Colombia. FAU - Morelos Gomez, Jose AU - Morelos Gomez J AUID- ORCID: 0000-0002-0334-0575 AD - CTIE (Centro de Transferencia, Innovacion y Emprendimiento of the Universidad de Cartagena), Universidad de Cartagena, Cartagena de Indias 130001, Colombia. LA - eng GR - BPIN Code 2021000100242/Sistema General de Regalias de Colombia/ GR - BPIN Code 2021000100255/Sistema General de Regalias de Colombia/ GR - BPIN Code 2021000100535/Sistema General de Regalias de Colombia/ PT - Journal Article DEP - 20261017 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700787 OTO - NOTNLM OT - Hardy-Weinberg equilibrium OT - allele frequency OT - calpain OT - calpastatin OT - casein OT - dual-purpose cattle OT - genomic characterization OT - selection index COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/17 CRDT- 2026/10/27 10:32 PHST- 2026/07/08 00:00 [received] PHST- 2026/08/05 00:00 [revised] PHST- 2026/08/08 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - ani16203270 [pii] AID - animals-16-03270 [pii] AID - 10.3390/ani16203270 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 17;16(20):3270. doi: 10.3390/ani16203270. PMID- 42939200 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 16 TI - Effects of Grain Source and Emulsifier Supplementation of Reduced-Energy Diets on Performance, Slaughter Traits, Tibia Characteristics, Jejunal Histomorphology and Pancreatic Enzymes in Broilers. LID - 10.3390/ani16203269 [doi] LID - 3269 AB - The purpose of this study was to evaluate the effect of emulsifier addition to reduced-energy maize-, barley- or wheat-based diets on selected indicators in broilers. In this experiment, a total of 540 unsexed (mixed-sex) one-day-old Ross 308 chicks were allocated to six treatment groups using a 3 x 2 factorial design (five replicate pens of 18 birds). Each diet was fed either as a standard-energy diet without emulsifier or as a reduced-energy diet (-50 kcal/kg ME, achieved by lowering soybean oil inclusion) supplemented with 500 g/ton emulsifier. The wheat-based diet reduced body weight and feed consumption but improved feed conversion ratio compared with the barley-based diet. Feeding a maize-based diet increased carcass yield and the breast (meat, skin and bone) ratio but decreased the leg (two thighs and two drumsticks, with meat, skin and bone) ratio compared to feeding a wheat-based diet. The addition of emulsifiers to barley- and wheat-based diets increased carcass yield. The negative effects of barley- and wheat-based diets on the carcass compared to the maize diet were compensated for by the addition of emulsifiers to the diet. The highest values for tibia breaking strength and tibia ash content were obtained with wheat-, maize-, and barley-based diets, respectively. Tibia ash increased with the addition of emulsifiers to barley- and wheat-based diets. Barley- and wheat-based diets resulted in higher pancreatic amylase activity compared to maize-based diets. Pancreatic lipase activity increased with the addition of emulsifiers. The wheat-based diet increased villus surface area compared to other cereals. The addition of emulsifier to the diet increased villus height/crypt depth but decreased other villus measurements. In conclusion, it can be said that adding emulsifiers to reduced-energy broiler diets feed is more effective in increasing carcass yield in barley- and wheat-based feeds, and that emulsifier addition compensates for the energy deficit in the diet. FAU - Olgun, Osman AU - Olgun O AUID- ORCID: 0000-0002-3732-1137 AD - Department of Animal Science, Faculty of Agriculture, Selcuk University, 42130 Konya, Turkiye. FAU - Cufadar, Yusuf AU - Cufadar Y AUID- ORCID: 0000-0001-9606-791X AD - Department of Animal Science, Faculty of Agriculture, Selcuk University, 42130 Konya, Turkiye. FAU - Gokmen, Seyit Ahmet AU - Gokmen SA AD - Department of Plant and Animal Production, Ladik Vocational School, Ondokuz Mayis University, 55500 Samsun, Turkiye. FAU - Gul, Esra Tugce AU - Gul ET AD - Department of Animal Science, Faculty of Agriculture, Selcuk University, 42130 Konya, Turkiye. FAU - Tuzun, Ahmet Engin AU - Tuzun AE AUID- ORCID: 0000-0003-3493-1623 AD - Kocarli Vocational School, Adnan Menderes University, 09970 Aydin, Turkiye. FAU - Selcuk, Bilal AU - Selcuk B AUID- ORCID: 0000-0001-9136-5707 AD - Department of Plant and Animal Production, Ladik Vocational School, Ondokuz Mayis University, 55500 Samsun, Turkiye. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700545 OTO - NOTNLM OT - barley OT - emulsifier OT - histomorphology OT - pancreatic enzymes OT - tibia COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/16 CRDT- 2026/10/27 10:32 PHST- 2026/09/08 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ani16203269 [pii] AID - animals-16-03269 [pii] AID - 10.3390/ani16203269 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 16;16(20):3269. doi: 10.3390/ani16203269. PMID- 42939192 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 16 TI - Refining the Protocol for Equine Umbilical-Cord-Derived Mesenchymal Stem Cell Isolation: A Pilot Study on Resource Efficiency and Cellular Properties. LID - 10.3390/ani16203258 [doi] LID - 3258 AB - Equine mesenchymal stem cells (MSCs) are vital tools for preclinical studies and regenerative medicine, due to their multipotent nature and immunomodulatory potential. Wharton's jelly provides a non-invasive and abundant source of MSCs. However, the isolation from equine umbilical cord remains challenging, as tissue decontamination is difficult and the enzymatic isolation methods often yield variable amounts of cells. In addition, the established protocols are frequently labour-intensive, time-consuming, and costly. In this pilot study we adapted existing protocols with the aim to improve contamination rates, time commitment and costs. A thorough decontamination method and increased collagenase I digestion time from 3 h in the reference method (RM) to 24 h for the novel method (NM) were employed. Trilineage differentiation, RT-qPCR and flow cytometry were used to characterise both groups. Finally, a non-inferiority analysis was performed to confirm the feasibility of the NM. The changes in protocol led to the decrease in overtime and nightshifts, a faster postisolation expansion by reducing the culture duration by 13.5 +/- 11 d, and minimised overall contamination rates (6.3% across 64 samples; RM = 3.1%, NM = 9.4%) compared with most published protocols. Consequently, we were able to reduce the costs per sample by 21 +/- 21%. FAU - Hollender, Annika-Celine AU - Hollender AC AUID- ORCID: 0000-0003-4359-9968 AD - Equine Clinic, Surgery and Radiology, School of Veterinary Medicine, Freie Universitat Berlin, Oertzenweg 19b, 14163 Berlin, Germany. AD - Institute of Veterinary Physiology, School of Veterinary Medicine, Freie Universitat Berlin, Konigsweg 56, 14163 Berlin, Germany. FAU - Stage, Hannah J AU - Stage HJ AUID- ORCID: 0009-0004-4639-3919 AD - Equine Clinic, Surgery and Radiology, School of Veterinary Medicine, Freie Universitat Berlin, Oertzenweg 19b, 14163 Berlin, Germany. FAU - Rausch, Sebastian AU - Rausch S AD - Institute of Immunology, School of Veterinary Medicine, Freie Universitat Berlin, Robert-von-Ostertag-Str. 7, 14163 Berlin, Germany. FAU - Trappe, Susanne AU - Trappe S AUID- ORCID: 0009-0005-2129-3396 AD - Institute of Veterinary Physiology, School of Veterinary Medicine, Freie Universitat Berlin, Konigsweg 56, 14163 Berlin, Germany. FAU - Dahl, Julius AU - Dahl J AD - Institute of Veterinary Physiology, School of Veterinary Medicine, Freie Universitat Berlin, Konigsweg 56, 14163 Berlin, Germany. FAU - Ockler, Tabea AU - Ockler T AD - Institute of Veterinary Physiology, School of Veterinary Medicine, Freie Universitat Berlin, Konigsweg 56, 14163 Berlin, Germany. FAU - Aschenbach, Jorg R AU - Aschenbach JR AUID- ORCID: 0000-0001-5080-1007 AD - Institute of Veterinary Physiology, School of Veterinary Medicine, Freie Universitat Berlin, Konigsweg 56, 14163 Berlin, Germany. FAU - Gehlen, Heidrun AU - Gehlen H AUID- ORCID: 0000-0001-5451-4232 AD - Equine Clinic, Surgery and Radiology, School of Veterinary Medicine, Freie Universitat Berlin, Oertzenweg 19b, 14163 Berlin, Germany. LA - eng GR - 470941103/Deutsche Forschungsgemeinschaft/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700546 OTO - NOTNLM OT - Wharton's jelly OT - cost-efficiency OT - equine OT - mesenchymal stem/stromal cells OT - microbial contamination OT - non-inferiority OT - processing time OT - uc-MSCs OT - umbilical cord COIS- The authors declare no conflicts of interest. The funding agency had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/16 CRDT- 2026/10/27 10:32 PHST- 2026/08/01 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ani16203258 [pii] AID - animals-16-03258 [pii] AID - 10.3390/ani16203258 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 16;16(20):3258. doi: 10.3390/ani16203258. PMID- 42939185 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 16 TI - Potential Feasibility and Perspectives of Fecal Microbiota Transplantation for Restoring Gut Function in Giant Pandas. LID - 10.3390/ani16203254 [doi] LID - 3254 AB - Although the giant panda (Ailuropoda melanoleuca) relies almost exclusively on bamboo, its carnivore-like gastrointestinal tract has limited capacity for fiber digestion. The gut microbiota contributes to bamboo-derived nutrient utilization, although the extent of this contribution remains limited. Captivity-associated dietary and environmental changes frequently disrupt this microbial ecosystem, resulting in impaired digestive function and reduced physiological resilience. Conventional interventions, including antibiotics and single-strain probiotics, are generally insufficient to restore the complexity and stability of the gut microbiota. Fecal microbiota transplantation (FMT), which reconstructs the gut microbial ecosystem by transferring an intact microbial community, has demonstrated therapeutic potential in humans and various animal models. However, its feasibility in giant pandas remains unclear because their short intestinal tract, absence of fermentation chambers, rapid gastrointestinal transit, and host-mediated ecological filtering substantially limit donor microbiota engraftment. This review evaluates the biological rationale and technical feasibility of oral FMT in giant pandas by integrating evidence from gastrointestinal physiology, microbial ecology, and conservation medicine. This review identifies the principal barriers to microbial engraftment. It synthesizes a giant panda-specific framework from the available literature, encompassing function-oriented donor selection, optimized microbial delivery strategies, and multidimensional efficacy assessment. Collectively, this synthesis provides a theoretical foundation for future FMT research in giant pandas and offers a conceptual reference for microbiota-based interventions in the conservation of endangered wildlife. FAU - Fu, Linxuan AU - Fu L AD - College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. AD - China Agricultural University-Sichuan Advanced Agricultural & Industrial Institute, Chengdu 611430, China. FAU - Xue, Yanzi AU - Xue Y AD - College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. AD - China Agricultural University-Sichuan Advanced Agricultural & Industrial Institute, Chengdu 611430, China. FAU - Wang, Yanghan AU - Wang Y AD - College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. FAU - Zhao, Shuoshuo AU - Zhao S AD - College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. FAU - Wang, Qianwei AU - Wang Q AD - College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. FAU - Che, Huilian AU - Che H AUID- ORCID: 0000-0002-1632-5729 AD - College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. AD - China Agricultural University-Sichuan Advanced Agricultural & Industrial Institute, Chengdu 611430, China. LA - eng GR - China Agricultural University-Sichuan Advanced Agricultural & Industrial Institute/ PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700554 OTO - NOTNLM OT - conservation medicine OT - fecal microbiota transplantation (FMT) OT - giant panda OT - gut microbiota OT - microbiome engineering COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/16 CRDT- 2026/10/27 10:32 PHST- 2026/08/08 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ani16203254 [pii] AID - animals-16-03254 [pii] AID - 10.3390/ani16203254 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 16;16(20):3254. doi: 10.3390/ani16203254. PMID- 42939184 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 16 TI - From Microbiome Associations to Deployable Precision Probiotics in Animals: A Critical Narrative Review and Proposed Causal Evidence Framework for Next-Generation Probiotic Development. LID - 10.3390/ani16203253 [doi] LID - 3253 AB - Next-generation probiotics (NGPs) are promoted as precision tools for animal health, yet the term is often applied to uncommon taxa before causality, safety and target-species efficacy are established. This critical narrative review reframes animal NGPs as a development pathway rather than a taxonomic category. A structured PubMed search was last run on 14 September 2026 with a publication date cutoff of 1 September 2026 and was supplemented by citation tracing and targeted searches of authoritative sources. From the screened literature, 45 representative and conceptually informative sources were purposively selected for the final narrative synthesis; the review was not intended to provide systematic or exhaustive coverage. The detailed synthesis focused on terrestrial farm and companion animals, whereas equine, rabbit and aquaculture studies were treated as boundary evidence. We evaluate how strain-resolved metagenomics, culturomics, metatranscriptomics, metaproteomics, metabolomics, host transcriptomics, machine learning, metabolic modeling and perturbation systems can move discovery beyond association. Studies across animal sectors show many biomarkers but limited causal validation, strain tracking, formulation data, multisite trials and ecological safety assessment. We propose an author-developed conceptual framework comprising a cumulative evidence ladder, from reproducible associations to field-ready deployment, and the ANIMAL-NGP reporting checklist (Animal endotype, Native ecological fit, Identity and genomic safety, Mechanism, Administration and manufacturing, and Longitudinal validation). These tools are intended to align claims with evidence and require prospective application and external validation. Multi-omics should therefore function as an evidence-navigation system, not as a substitute for causality. FAU - Ho, Shang-Tse AU - Ho ST AUID- ORCID: 0000-0002-1838-7173 AD - Department of Wood Based Materials and Design, National Chiayi University, Chiayi City 600355, Taiwan. FAU - Chen, Ming-Ju AU - Chen MJ AUID- ORCID: 0000-0003-4403-6112 AD - Department of Animal Science and Technology, National Taiwan University, Taipei 10617, Taiwan. LA - eng GR - NSTC 115-2313-B-002-006-MY3/National Science and Technology Council/ PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700747 OTO - NOTNLM OT - animal microbiome OT - antimicrobial resistance OT - culturomics OT - engineered probiotics OT - metabolomics OT - multi-omics OT - next-generation probiotics OT - one health OT - precision probiotics OT - strain tracking COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/16 CRDT- 2026/10/27 10:32 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - ani16203253 [pii] AID - animals-16-03253 [pii] AID - 10.3390/ani16203253 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 16;16(20):3253. doi: 10.3390/ani16203253. PMID- 42939180 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 15 TI - Transcriptome Analysis Reveals the Effects of Dietary DHA and EPA on the Hepatic Response to Unpredictable Chronic Stress in Atlantic Salmon (Salmo salar L.). LID - 10.3390/ani16203249 [doi] LID - 3249 AB - Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are key long-chain omega-3 fatty acids in fish nutrition, but their differential effects on Atlantic salmon under unpredictable chronic stress (UCS) remain unclear. This study compared hepatic responses to DHA- and EPA-rich diets under unstressed and UCS conditions. Atlantic salmon post-smolts were fed a low-EPA+DHA, DHA-rich, or EPA-rich diet for 10 weeks, followed by 3 weeks of UCS. Liver transcriptomes were analyzed by RNA sequencing, and selected genes were validated by quantitative reverse transcription PCR. Growth did not differ among dietary groups. Under unstressed conditions, the DHA-rich diet induced more differentially expressed genes than the EPA-rich diet (67 vs. 29) and affected pathways related to fatty acid metabolism, autophagy, cholesterol homeostasis, and antioxidant defense. After UCS, the EPA-rich diet induced a broader hepatic transcriptional response than the DHA-rich diet (205 vs. 57 differentially expressed genes), with only 12 genes shared between the two comparisons. Both diets affected fatty acid- and steroid-biosynthesis-related pathways, while gene-level responses were diet-specific. These findings reveal distinct, stress-dependent hepatic responses to dietary DHA and EPA and may inform future optimization of their nutritional provision in Atlantic salmon production. FAU - Deng, Yajie AU - Deng Y AD - College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China. AD - Liaoning Provincial Key Laboratory of Northern Aquatic Germplasm Resources and Genetics and Breeding, Dalian Ocean University, Dalian 116023, China. FAU - Chen, Jian AU - Chen J AD - College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China. AD - Liaoning Provincial Key Laboratory of Northern Aquatic Germplasm Resources and Genetics and Breeding, Dalian Ocean University, Dalian 116023, China. FAU - Su, Hang AU - Su H AUID- ORCID: 0000-0003-0571-0864 AD - College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China. AD - Liaoning Provincial Key Laboratory of Northern Aquatic Germplasm Resources and Genetics and Breeding, Dalian Ocean University, Dalian 116023, China. AD - Chinese-Norwegian Joint Laboratory for Development and Utilization of Marine Bioresource and Sustainable Aquaculture, Dalian Ocean University, Dalian 116023, China. AD - NIBIO-Norwegian Institute of Bioeconomy Research, 1431 As, Norway. FAU - Zhang, Yupeng AU - Zhang Y AUID- ORCID: 0000-0001-9461-8554 AD - NIBIO-Norwegian Institute of Bioeconomy Research, 1431 As, Norway. FAU - Ding, Jun AU - Ding J AUID- ORCID: 0000-0003-2632-7768 AD - College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China. AD - Liaoning Provincial Key Laboratory of Northern Aquatic Germplasm Resources and Genetics and Breeding, Dalian Ocean University, Dalian 116023, China. FAU - Clarke, Jihong Liu AU - Clarke JL AUID- ORCID: 0000-0002-4924-0406 AD - NIBIO-Norwegian Institute of Bioeconomy Research, 1431 As, Norway. LA - eng GR - 336168/The Research Council of Norway/ GR - CHN-2152, 22/0013/Ministry of Foreign Affairs/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700733 OTO - NOTNLM OT - Atlantic salmon OT - DHA OT - EPA OT - fatty acid metabolism OT - highly unsaturated fatty acids OT - oxidative stress OT - unpredictable chronic stress COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/15 CRDT- 2026/10/27 10:32 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ani16203249 [pii] AID - animals-16-03249 [pii] AID - 10.3390/ani16203249 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 15;16(20):3249. doi: 10.3390/ani16203249. PMID- 42939179 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 15 TI - Greenhouse Gas Emissions from Large-Scale Dairy Farms: Interannual Dynamics, Farm-Level Heterogeneity, and Key Determinants. LID - 10.3390/ani16203248 [doi] LID - 3248 AB - The transition to low-emission (LE) dairy production is essential for mitigating agricultural greenhouse gas (GHG) emissions. However, the interannual variability and principal drivers of farm-level GHG footprints across intensive dairy farms remain insufficiently characterized, constraining targeted mitigation strategies. This study aimed to (i) quantify interannual variation in on-farm GHG emission intensity, (ii) characterize management contrasts between high- and low-emission farms, (iii) clarify the underlying mechanisms, and (iv) identify optimization pathways. A supplementary sensitivity analysis encompassing upstream emissions associated with feed production was performed using an expanded system boundary to assess the effects of system boundary selection on the estimated GHG emissions. Using 3-year (2021-2023) field data from 22 intensive dairy farms, a standardized farm-level GHG inventory was used to quantify operational GHG emission intensity per unit of fat- and protein-corrected milk (FPCM). The production-weighted mean on-farm operational GHG intensity was 0.86 kg CO(2)-eq kg(-1) FPCM, with enteric methane constituting the dominant emission source. Incorporating upstream emissions associated with feed production contributed an additional 0.427 kg CO(2)-eq kg(-1) FPCM, yielding a total emission intensity of 1.287 kg CO(2)-eq kg(-1) FPCM under the expanded system boundary, representing a 49.7% increase relative to the operational inventory. Feed upstream emissions accounted for 33.2% of the total emissions estimated within the expanded system boundary. Both herd size and energy consumption exhibited increasing trends throughout the study period, whereas the proportion of lactating cows relating to the total herd declined from 46.01% in 2021 to 44.32% in 2023, suggesting that the expansion of the overall herd exceeded the corresponding growth in the lactating cow population. Under the operational system boundary, high-emission (HE) farms exhibited relatively greater contributions to their GHG emission profiles from enteric methane production, electricity consumption, and manure management activities. Conversely, LE farms attained improved performance through higher proportions of lactating cows, greater feed efficiency, and manure anaerobic digestion. Correlation analysis linked herd size, energy consumption, and dry matter intake positively to on-farm GHG emissions. Dietary starch was associated with manure N(2)O emissions, while feed efficiency was associated with higher energy-related GHG emissions. Overall, herd composition, feeding management practices, energy utilization, and manure management strategies were identified as principal determinants of on-farm GHG emission performance. Furthermore, the sensitivity analysis employing an expanded system boundary demonstrated that incorporating upstream emissions associated with feed production substantially elevates the overall GHG emission intensity expressed per kg FPCM. This study helps address the lack of multi-year farm-level evidence for China's dairy sector and proposes integrated strategies-herd structure adjustment, precision feeding, clean energy exploitation, and manure management-alongside sustained long-term monitoring, given the pronounced interannual variability. These findings highlight the roles of farm-level GHG inventories in identifying targeted on-farm emission mitigation opportunities and expanded system boundary assessments in quantifying the overall GHG burden associated with the entire dairy production supply chain. FAU - Shen, Yueyu AU - Shen Y AD - Institute of Feed Research, Chinese Academy of Agricultural Sciences/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Beijing 100081, China. FAU - Su, Xiaodong AU - Su X AUID- ORCID: 0009-0006-4876-1572 AD - College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China. FAU - Ma, Yadong AU - Ma Y AD - Institute of Feed Research, Chinese Academy of Agricultural Sciences/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Beijing 100081, China. FAU - Liu, Yunlong AU - Liu Y AD - Institute of Feed Research, Chinese Academy of Agricultural Sciences/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Beijing 100081, China. FAU - Zhang, Xin AU - Zhang X AD - Institute of Feed Research, Chinese Academy of Agricultural Sciences/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Beijing 100081, China. FAU - Muhammad, Zeeshan AU - Muhammad Z AUID- ORCID: 0000-0001-9334-802X AD - Institute of Feed Research, Chinese Academy of Agricultural Sciences/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Beijing 100081, China. FAU - Asif, Sana AU - Asif S AUID- ORCID: 0000-0002-4378-9895 AD - Institute of Feed Research, Chinese Academy of Agricultural Sciences/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Beijing 100081, China. FAU - Pei, Di AU - Pei D AD - Beijing Sunlon Livestock Development Company Limited, Beijing 100176, China. FAU - Tu, Yan AU - Tu Y AUID- ORCID: 0000-0002-4324-6188 AD - Institute of Feed Research, Chinese Academy of Agricultural Sciences/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Beijing 100081, China. AD - Beijing Innovation Consortium of Livestock Research System (BAIC05), Beijing 100081, China. LA - eng GR - BAIC05/Beijing Innovation Consortium of Livestock Research System/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700813 OTO - NOTNLM OT - carbon emission intensity OT - greenhouse gas footprint OT - interannual variability OT - life cycle assessment OT - manure management COIS- Author Di Pei is affiliated with Beijing Sunlon Livestock Development Company Limited, Beijing, China. The remaining authors declare that this research was conducted in the absence of any commercial or financial relationships that could be construed as potential conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/15 CRDT- 2026/10/27 10:32 PHST- 2026/08/28 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ani16203248 [pii] AID - animals-16-03248 [pii] AID - 10.3390/ani16203248 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 15;16(20):3248. doi: 10.3390/ani16203248. PMID- 42939174 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 15 TI - Machine-Learning-Based Genomic Prioritization and Genome-Wide Association Analysis of Growth Traits in Central Anatolian Merino Sheep. LID - 10.3390/ani16203243 [doi] LID - 3243 AB - Growth traits are major economic drivers in sheep production, yet their genetic architecture is highly polygenic and influenced by multiple genetic and environmental factors. Here, we integrated machine-learning-based genomic prioritization and prediction with conventional mixed-model genome-wide association analysis to investigate growth traits in Central Anatolian Merino sheep. Phenotypic records for birth weight (BW), weaning weight (WW), six-month weight (W6), and average daily gains before (ADWG1) and after weaning (ADWG2) were obtained from 278 lambs genotyped using the Illumina OvineSNP50 BeadChip. Following genotype quality control, 275 animals and 46,902 SNPs were retained for genome-wide analyses. Machine-learning models were evaluated using five-fold outer cross-validation repeated five times. Within each outer training set, SNP preprocessing, feature prioritization, consensus ranking, marker-panel construction, hyperparameter optimization, and model fitting were performed independently. LASSO, Elastic Net, Random Forest, and XGBoost were used for SNP prioritization, and 50-, 100-, and 200-SNP panels were evaluated using Ridge regression, Random Forest, XGBoost, and Support Vector Regression. Conventional mixed-model GWAS identified no SNP reaching genome-wide significance after Bonferroni or FDR correction, although several suggestive and chromosome-wide signals were detected. Exploratory comparison of ML prioritization and GWAS results revealed concordant genomic signals for related traits, particularly ADWG1-WW and ADWG2-W6; these were interpreted as shared genomic signals rather than evidence of pleiotropy. Candidate gene annotation identified biologically relevant positional candidate genes involved in muscle development, skeletal growth, metabolism, and cellular regulation, including PRMT1, TNC, POU1F1, RASD2, ERCC6L2, SSH2, and DYM. Overall, the complementary use of machine-learning-based genomic prioritization and conventional GWAS provided a conservative framework for investigating the genomic architecture of growth traits. The identified genomic signals and positional candidate genes should be considered exploratory and require validation in larger and independent sheep populations before their potential application in genomic selection. FAU - Arzik, Yunus AU - Arzik Y AUID- ORCID: 0000-0002-3068-8155 AD - Department of Animal Science, Faculty of Veterinary Medicine, Aksaray University, 68100 Aksaray, Turkiye. FAU - Kizilaslan, Mehmet AU - Kizilaslan M AD - Department of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA. FAU - Behrem, Sedat AU - Behrem S AD - Department of Animal Science, Faculty of Veterinary Medicine, Aksaray University, 68100 Aksaray, Turkiye. FAU - Cinar, Mehmet Ulas AU - Cinar MU AUID- ORCID: 0000-0001-5894-5072 AD - Department of Animal Science, Faculty of Agriculture, Erciyes University, 38280 Kayseri, Turkiye. AD - Department of Veterinary Microbiology & Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA 99164, USA. AD - Betul-Ziya Eren Genome and Stem Cell Research Center (GENKOK), Erciyes University, 38280 Kayseri, Turkiye. LA - eng GR - FBAU-2023-12523/Erciyes University/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700748 OTO - NOTNLM OT - Ovis aries OT - SNP prioritization OT - genome-wide association study OT - genomic prediction OT - growth traits OT - machine learning COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/15 CRDT- 2026/10/27 10:32 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - ani16203243 [pii] AID - animals-16-03243 [pii] AID - 10.3390/ani16203243 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 15;16(20):3243. doi: 10.3390/ani16203243. PMID- 42939166 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 14 TI - Rumen-Protected Niacin Supplementation Modulates Uterine Arterial Hemodynamics and Endometrial Microbiome Functional Potential in Postpartum Japanese Black Cattle. LID - 10.3390/ani16203235 [doi] LID - 3235 AB - This study examined whether rumen-protected niacin (RPN) supplementation is associated with uterine hemodynamics and the postpartum uterine environment in Japanese Black cattle. Experiment 1 used a crossover design to evaluate acute uterine vascular responses to RPN in synchronized heifers (n = 6). Experiment 2 was an exploratory evaluation of postpartum cows (n = 6) during long-term RPN supplementation using uterine artery Doppler measurements, uterine inflammatory indicators, and endometrial microbiome analysis. In Experiment 1, high-dose RPN administration was associated with changes in uterine hemodynamic parameters (p < 0.05) and higher baseline-adjusted whole-blood NAD(+) concentrations across the post-administration period (p < 0.001). In Experiment 2, RPN supplementation was associated with time-dependent differences in uterine hemodynamic parameters, whereas uterine inflammatory indicators were not significantly affected. Although broad treatment-associated differences in overall endometrial microbial diversity and community structure were not detected, exploratory differences in specific bacterial taxa were observed between treatment groups, and predicted metabolic pathways showed treatment-dependent temporal changes. Exploratory integrated analysis further identified concurrent within-animal patterns across uterine hemodynamics, microbial community structure, and predicted microbial functional potential. These exploratory findings suggest that RPN may modulate uterine hemodynamics and be associated with selective changes in the postpartum uterine environment, providing a basis for further investigation of the relationships among nutritional intervention, uterine hemodynamics, the endometrial microbial ecosystem, and subsequent reproductive physiology. FAU - Yorozui, Yuki AU - Yorozui Y AD - Department of Animal Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan. FAU - Inoue, Seiji AU - Inoue S AD - Department of Animal Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan. FAU - Mu, Yufei AU - Mu Y AD - Department of Bioresource Sciences, Graduate School of Bioresource and Environmental Studies, Kyushu University, Fukuoka 819-0395, Japan. FAU - Matsuyama, Shuichi AU - Matsuyama S AD - Department of Animal Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan. FAU - Ohkura, Satoshi AU - Ohkura S AUID- ORCID: 0000-0002-0052-8951 AD - Department of Animal Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan. FAU - Morita, Yasuhiro AU - Morita Y AUID- ORCID: 0000-0001-7364-3187 AD - Department of Bioresource Sciences, Graduate School of Bioresource and Environmental Studies, Kyushu University, Fukuoka 819-0395, Japan. LA - eng GR - KEN82/Ito Foundation of Japan/ GR - 20K15647/Japan Society for the Promotion of Science/ GR - 24K09209/Japan Society for the Promotion of Science/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700562 OTO - NOTNLM OT - niacin OT - uterine environment OT - uterine hemodynamics OT - uterine microbiome COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:32 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - ani16203235 [pii] AID - animals-16-03235 [pii] AID - 10.3390/ani16203235 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 14;16(20):3235. doi: 10.3390/ani16203235. PMID- 42939161 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 14 TI - Quantification of Fecal Glucocorticoid Metabolites in Shelter Cats Using LC/MS/MS: Potential Biomarkers of Stress Associated with Shelter Admission. LID - 10.3390/ani16203227 [doi] LID - 3227 AB - Cats can experience physiological stress during transitions to unfamiliar environments, such as shelter admission. To enable non-invasive stress assessment in cats, fecal glucocorticoid metabolites were quantified by liquid chromatography-tandem mass spectrometry (LC/MS/MS) with Girard's reagent T derivatization. Five analytes were targeted: three glucocorticoids (cortisol, cortisone, and corticosterone) and their fecal metabolites, 11beta-hydroxyetiocholanolone and 11-oxoetiocholanolone. Thirty-five cats housed at an animal welfare center were classified into a shorter-admission group (<2 weeks after admission, n = 16) or a longer-admission group (>/=2 weeks, n = 19). The three parent glucocorticoids showed no detectable peak in any sample, whereas both metabolites were detected in 28 of the 35 samples and were strongly correlated in both the shorter- and longer-admission groups (rho = 0.9316 and 0.9699, respectively). Both metabolites were significantly higher in the shorter-admission group (p = 0.0036 and 0.0029, respectively), whereas no significant differences were detected by sex or neuter status. In an incubation experiment, concentration changes within 48 h ( 0.05), whereas chromatin integrity was higher after slow freezing (p = 0.03). Rapid freezing reduced acrosomal area, acrosomal perimeter, and head length, whereas slow freezing preserved morphometric variables and correlations, although correlation patterns should be interpreted as associations among morphometric traits rather than structural preservation. Both cooling curves were well described by linear and cubic models. Slow freezing was associated with better preservation of chromatin integrity and selected morphometric characteristics. FAU - Castaneda-Pastor, Yanez M AU - Castaneda-Pastor YM AUID- ORCID: 0009-0007-6125-4863 AD - Facultad de Ingenieria Zootecnista, Biotecnologia, Agronegocios y Ciencia de Datos, Universidad Nacional Toribio Rodriguez de Mendoza de Amazonas, Chachapoyas 01001, Amazonas, Peru. FAU - Cayo-Colca, Ilse S AU - Cayo-Colca IS AUID- ORCID: 0000-0001-6518-0979 AD - Facultad de Ingenieria Zootecnista, Biotecnologia, Agronegocios y Ciencia de Datos, Universidad Nacional Toribio Rodriguez de Mendoza de Amazonas, Chachapoyas 01001, Amazonas, Peru. FAU - Maicelo-Quintana, Jorge L AU - Maicelo-Quintana JL AD - Facultad de Ingenieria Zootecnista, Biotecnologia, Agronegocios y Ciencia de Datos, Universidad Nacional Toribio Rodriguez de Mendoza de Amazonas, Chachapoyas 01001, Amazonas, Peru. FAU - Maravi-Carmen, Cesar A AU - Maravi-Carmen CA AUID- ORCID: 0000-0003-2603-8632 AD - Facultad de Ingenieria Zootecnista, Biotecnologia, Agronegocios y Ciencia de Datos, Universidad Nacional Toribio Rodriguez de Mendoza de Amazonas, Chachapoyas 01001, Amazonas, Peru. AD - Instituto de Investigacion en Ganaderia y Biotecnologia, Chachapoyas 01001, Amazonas, Peru. FAU - Quispe-Ccasa, Hurley Abel AU - Quispe-Ccasa HA AUID- ORCID: 0000-0003-3146-5372 AD - Direccion de Investigacion y Desarrollo Tecnologico, Instituto Nacional de Innovacion Agraria, La Molina, Lima 15024, Peru. FAU - Briceno-Mendoza, Yander M AU - Briceno-Mendoza YM AUID- ORCID: 0000-0001-5535-8091 AD - Facultad de Ingenieria Zootecnista, Biotecnologia, Agronegocios y Ciencia de Datos, Universidad Nacional Toribio Rodriguez de Mendoza de Amazonas, Chachapoyas 01001, Amazonas, Peru. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700794 OTO - NOTNLM OT - Cavia porcellus OT - epididymal spermatozoa OT - freezing curve OT - refrigeration OT - semen quality OT - sperm cryopreservation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/13 CRDT- 2026/10/27 10:32 PHST- 2026/07/28 00:00 [received] PHST- 2026/08/21 00:00 [revised] PHST- 2026/09/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - ani16203211 [pii] AID - animals-16-03211 [pii] AID - 10.3390/ani16203211 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 13;16(20):3211. doi: 10.3390/ani16203211. PMID- 42939139 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 12 TI - Investigating the Relationship Between Hoof, Liver, and Cardiac Abnormalities in Bos taurus Grain-Fed Beef Cattle < 30 Months in a Natural Program with No Antibiotics or Growth Promotants. LID - 10.3390/ani16203208 [doi] LID - 3208 AB - Our primary objective was to determine the relationship between hoof, liver, and cardiac abnormalities in a group of natural-fed cattle at slaughter. Bos taurus beef cattle (N = 1312), without dairy phenotype, were evaluated for abnormalities at a slaughter establishment in the Great Plains region of the United States (elevation: 563 m). Cattle were enrolled in a vegetarian-fed program that prohibited implants, diet-included hormones, and beta-adrenergic agonists. Generalized linear mixed models were used to determine the prevalence of each abnormality within other abnormalities. There was no detectable difference (p >/= 0.1630) to support an association between the proportion of cattle with each hoof score within each heart or liver score. Cattle with no liver abscesses were more prevalent (p = 0.0355) among cattle with normal cardiac morphology (Heart Score (HS)1) (94.37 +/- 1.19% (mean +/- SE)) than cattle with HS2 (very mild remodeling) (88.6 +/- 1.42%). Further research is needed to understand this observed difference between two similar heart scores and to further explore contributing factors to these production diseases. FAU - Hamilton, Emma M AU - Hamilton EM AD - Department of Animal Sciences, Colorado State University, Fort Collins, CO 80523-1171, USA. FAU - Anderson, Karly N AU - Anderson KN AUID- ORCID: 0000-0003-4997-997X AD - Department of Animal and Food Science, University of Wisconsin-River Falls, River Falls, WI 54022, USA. AD - Humane Handling Institute, University of Wisconsin-River Falls, River Falls, WI 54022, USA. FAU - Vogel, Kurt D AU - Vogel KD AUID- ORCID: 0000-0001-8712-7298 AD - Department of Animal and Food Science, University of Wisconsin-River Falls, River Falls, WI 54022, USA. AD - Humane Handling Institute, University of Wisconsin-River Falls, River Falls, WI 54022, USA. FAU - Grandin, Temple AU - Grandin T AUID- ORCID: 0000-0001-7231-7101 AD - Department of Animal Sciences, Colorado State University, Fort Collins, CO 80523-1171, USA. LA - eng GR - Grandin Livestock Handling Systems/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700788 OTO - NOTNLM OT - cardiac remodeling OT - cattle OT - corkscrew hoof OT - liver abscess OT - natural OT - welfare COIS- The last author is a paid welfare consultant for McDonald's Corporation and other large companies. The author has no conflicts of interest on issues related to the selection of breeding cattle, genetic testing, or treatments for the production diseases discussed in this manuscript. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:32 PHST- 2026/08/15 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - ani16203208 [pii] AID - animals-16-03208 [pii] AID - 10.3390/ani16203208 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 12;16(20):3208. doi: 10.3390/ani16203208. PMID- 42939134 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 12 TI - Superior MAP-EtCO(2) Target Attainment with Rocuronium-Facilitated Controlled Ventilation in Geriatric Small Dogs: A Retrospective Cohort Study. LID - 10.3390/ani16203203 [doi] LID - 3203 AB - Comparative evidence for integrated ventilation-neuromuscular blockade-reversal strategies is limited in geriatric small dogs. This single-center retrospective cohort included 288 dogs aged >/=12 years and weighing /= 60 mmHg and end-tidal carbon dioxide 35-55 mmHg. Adjusted means were A 58.9%, B 59.3%, C 86.6%, D 74.0%, and E 67.8%. Group C exceeded A, B, and D by 27.7 (95% confidence interval, 26.3-29.2), 27.3 (25.9-28.8), and 12.7 (11.2-14.1) percentage points; B and A differed by 0.4 points (-0.8-1.6). Ventilator asynchrony occurred in 3.4% of C versus 73.2% of B. Adjusted mean times to normalized train-of-four ratio >/= 0.90 were 1.41, 2.31, and 7.43 min in Groups C-E, respectively. Observed early respiratory and hard 48 h event risks were 3.4% and 1.7% in C, 40.7% and 18.6% in A, and 44.6% and 17.9% in B. The rocuronium-facilitated strategy was associated with superior concurrent target attainment; nonrandomized strategy selection precludes causal safety conclusions. FAU - Kim, Seung-Hyun AU - Kim SH AUID- ORCID: 0009-0008-9285-9264 AD - KSH Surgical Animal Medical Center, Gwangju 61617, Republic of Korea. FAU - Baek, Eun-Ji AU - Baek EJ AD - KSH Surgical Animal Medical Center, Gwangju 61617, Republic of Korea. FAU - Bae, Chun-Sik AU - Bae CS AUID- ORCID: 0000-0002-3631-7224 AD - Department of Veterinary Surgery, College of Veterinary Medicine, Chonnam National University, Gwangju 61186, Republic of Korea. LA - eng PT - Journal Article DEP - 20261012 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700846 OTO - NOTNLM OT - controlled ventilation OT - end-tidal carbon dioxide OT - geriatric dogs OT - mean arterial pressure OT - quantitative neuromuscular monitoring OT - rocuronium OT - sugammadex COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:32 PHST- 2026/08/14 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - ani16203203 [pii] AID - animals-16-03203 [pii] AID - 10.3390/ani16203203 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 12;16(20):3203. doi: 10.3390/ani16203203. PMID- 42939133 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 12 TI - Spatiotemporal Dynamics and Trend Analysis of African Swine Fever Virus in Romanian Wild Boar Populations (2018-2024). LID - 10.3390/ani16203202 [doi] LID - 3202 AB - Background: African Swine Fever (ASF) remains a critical threat to the swine industry globally, with wild boars (Sus scrofa) acting as a major wildlife reservoir. Romania has been one of the most severely affected European countries since the virus's introduction. Objectives: This study aims to estimate the long-term spatiotemporal trends, environmental persistence, and transmission dynamics of ASF virus (ASFV) in the Romanian wild boar population based on a finalized dataset of 1443 confirmed cases distributed across an 80-month timeline (May 2018-December 2024). Methods: Advanced epidemiological workflows integrating spatial and temporal modeling frameworks were developed. Spatiotemporal cluster detection was performed using SatScan to identify significant disease hotspots. Generalized Additive Models (GAMs) were utilized to model non-linear seasonal trends and spatial patterns of carcass persistence. Furthermore, the time-varying reproduction number (R(t)) was calculated using a Bayesian renewal framework via the EpiEstim package, parameterized with a wild boar serial distribution (mean = 10.3 days; SD = 4.2 days). Results: SatScan identified one most primary cluster in Dobrogea and five other major recurrent hot spots in Muntenia, Transylvania, and Moldova. The GAM analysis revealed a strong, statistically significant seasonal pattern, with case detection from carcasses peaking markedly during the winter months when low temperatures favor environmental virus stability. The Bayesian R(t) reconstruction disclosed three distinct epidemiological phases: a sharp initial invasion peak at a mean R(t) of 2.38 (95% Crl: 1.82-3.02), followed by regional wave-like oscillations, and a distinct long-term stabilization in the late endemic phase, hovering dynamically around a stationary mean of 1.01 (95% Crl: 0.87-1.16). Conclusions: Our findings statistically validate the shift from an acute epidemic wave to a self-sustaining, chronic endemic equilibrium within the natural habitat, driven by constant carcass-mediated environmental re-seeding. These insights underscore that successful disease mitigation must prioritize the rapid, targeted removal of infected carcasses in high-risk forest matrices rather than generic population reduction strategies. FAU - Pascu, Corina AU - Pascu C AUID- ORCID: 0000-0002-1013-0437 AD - Department of Infectious Diseases and Preventive Medicine, Faculty of Veterinary Medicine, University of Life Sciences "King Mihai I", 300645 Timisoara, Romania. FAU - Badea, Corina AU - Badea C AUID- ORCID: 0009-0009-1951-3635 AD - Department of Infectious Diseases and Preventive Medicine, Faculty of Veterinary Medicine, University of Life Sciences "King Mihai I", 300645 Timisoara, Romania. FAU - Herman, Viorel AU - Herman V AUID- ORCID: 0000-0001-7118-6488 AD - Department of Infectious Diseases and Preventive Medicine, Faculty of Veterinary Medicine, University of Life Sciences "King Mihai I", 300645 Timisoara, Romania. FAU - Imre, Kalman AU - Imre K AUID- ORCID: 0000-0002-6057-882X AD - Department of Food Safety and Hygiene, Faculty of Veterinary Medicine, University of Life Sciences "King Mihai I", 300645 Timisoara, Romania. FAU - Barbuceanu, Florica AU - Barbuceanu F AUID- ORCID: 0000-0001-9929-2602 AD - Institute for Diagnosis and Animal Health (IDAH), 050557 Bucharest, Romania. AD - Faculty of Veterinary Medicine, University of Agronomical Sciences and Veterinary Medicine, 050097 Bucharest, Romania. FAU - Tanase, Oana AU - Tanase O AD - Department of Public Health, "Ion Ionescu de la Brad" Iasi University of Life Sciences, 8 Sadoveanu Alley, 707027 Iasi, Romania. FAU - Costinar, Luminita AU - Costinar L AUID- ORCID: 0000-0001-9871-9248 AD - Department of Infectious Diseases and Preventive Medicine, Faculty of Veterinary Medicine, University of Life Sciences "King Mihai I", 300645 Timisoara, Romania. LA - eng GR - ADER 10.1.1./Ministry of Agriculture and Rural Development/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700671 OTO - NOTNLM OT - African Swine Fever OT - Generalized Additive Models (GAMs) OT - Romania OT - SatScan OT - epidemiology OT - spatiotemporal trend OT - wild boar COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:32 PHST- 2026/09/08 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - ani16203202 [pii] AID - animals-16-03202 [pii] AID - 10.3390/ani16203202 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 12;16(20):3202. doi: 10.3390/ani16203202. PMID- 42939127 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 12 TI - Construction and Evaluation of a Ketosis Prediction Model Based on Milk MIR Spectra and Feature Wavenumber Selection in Dairy Cows. LID - 10.3390/ani16203196 [doi] LID - 3196 AB - Ketosis is a common metabolic disorder in dairy cows and occurs mainly during early lactation. Blood beta-hydroxybutyrate (BHB) is widely used for diagnosis, but large-scale testing is limited by sampling requirements and cost. We therefore studied lactating Holstein cows from 12 large-scale dairy farms in central China. Milk samples, milk composition traits, and milk mid-infrared (MIR) spectra were collected through the Dairy Herd Improvement (DHI) testing system from April 2019 to December 2022, and ketosis diagnoses were obtained from farm health records; samples were labeled as healthy or ketotic based on the temporal relationship between diagnosis and sampling. A total of 2206 samples were used to build the calibration and validation datasets, in which five feature wavenumber selection methods and five machine learning algorithms were compared. Ketotic cows had a higher milk fat percentage and fat-to-protein ratio and a lower protein and lactose percentage (p < 0.01). The best combination was PLS-DA based on 104 CARS-selected wavenumbers, which achieved a sensitivity of 0.83, a specificity of 0.79, a balanced accuracy of 0.81 and an area under the receiver operating characteristic curve (AUC) of 0.88 in validation. MIR spectroscopy combined with machine learning can therefore support large-scale ketosis phenotyping within the DHI system. FAU - Ren, Xiaoli AU - Ren X AUID- ORCID: 0009-0005-1656-0840 AD - Frontiers Science Center for Animal Breeding and Sustainable Production, Huazhong Agricultural University, Ministry of Education, Wuhan 430070, China. AD - Henan Provincial International Joint Laboratory for Dairy Health Farming, Henan Dairy Herd Improvement Center, Zhengzhou 450046, China. FAU - Yang, Zhuo AU - Yang Z AD - Frontiers Science Center for Animal Breeding and Sustainable Production, Huazhong Agricultural University, Ministry of Education, Wuhan 430070, China. FAU - Han, Aote AU - Han A AUID- ORCID: 0009-0008-3465-6370 AD - Frontiers Science Center for Animal Breeding and Sustainable Production, Huazhong Agricultural University, Ministry of Education, Wuhan 430070, China. FAU - Fan, Yikai AU - Fan Y AUID- ORCID: 0009-0007-0398-4702 AD - Frontiers Science Center for Animal Breeding and Sustainable Production, Huazhong Agricultural University, Ministry of Education, Wuhan 430070, China. AD - Information Technology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. FAU - Zhang, Yi AU - Zhang Y AUID- ORCID: 0000-0003-1642-5890 AD - College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. FAU - Chen, Shaohu AU - Chen S AD - Dairy Association of China, Beijing 100193, China. FAU - Zhang, Zhen AU - Zhang Z AD - Henan Seed Industry Development Center, Zhengzhou 450046, China. FAU - Yan, Lei AU - Yan L AD - Henan Seed Industry Development Center, Zhengzhou 450046, China. FAU - Cao, Zheng AU - Cao Z AD - Dairy Association of China, Beijing 100193, China. FAU - Liu, Wenjiao AU - Liu W AD - Dairy Association of China, Beijing 100193, China. FAU - Huang, Xiaofeng AU - Huang X AD - Frontiers Science Center for Animal Breeding and Sustainable Production, Huazhong Agricultural University, Ministry of Education, Wuhan 430070, China. FAU - Wang, Dongwei AU - Wang D AD - Frontiers Science Center for Animal Breeding and Sustainable Production, Huazhong Agricultural University, Ministry of Education, Wuhan 430070, China. FAU - Zhang, Guixiang AU - Zhang G AD - National Animal Husbandry Service, Beijing 100125, China. FAU - Yan, Qingxia AU - Yan Q AD - Dairy Association of China, Beijing 100193, China. FAU - Zhang, Shujun AU - Zhang S AUID- ORCID: 0000-0002-5357-445X AD - Frontiers Science Center for Animal Breeding and Sustainable Production, Huazhong Agricultural University, Ministry of Education, Wuhan 430070, China. LA - eng GR - 2023YFD1300400/National Key R&D Program of China/ GR - No. 262102110074/Henan Provincial Science and Technology Research and Development/ GR - XZ202502ZY0002/Science and Teleology Projects of Xizang Autonomous Region, China/ GR - No. 221111111100/Key Research Project of Henan Province/ GR - No. HARS-22-14-S/the Special Fund for the Henan Agriculture Research System/ GR - No. 2026-PC-03/the Beijing Postdoctoral Research Funding/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700896 OTO - NOTNLM OT - dairy cows OT - ketosis OT - machine learning OT - mid-infrared spectra OT - phenotypic detection models COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:32 PHST- 2026/08/07 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - ani16203196 [pii] AID - animals-16-03196 [pii] AID - 10.3390/ani16203196 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 12;16(20):3196. doi: 10.3390/ani16203196. PMID- 42939118 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 11 TI - Chorus Size Shapes Calling Effort in the Asiatic Toad (Bufo gargarizans). LID - 10.3390/ani16203187 [doi] LID - 3187 AB - Acoustic communication is a core component of reproductive behavior in anuran amphibians. Among the factors shaping male calling behavior, the social acoustic environment plays a decisive role, primarily through chorus size; yet its regulatory effect in explosive-breeding species remains poorly understood. To investigate how chorus size affects the calling behavior of the Asiatic toad (Bufo gargarizans), we conducted a controlled experiment with two chorus-size treatments, small (five individuals per box) and large (15 individuals per box), corresponding to densities of approximately 13 and 39 individuals/m(2), each replicated across three nights using unique individuals. We compared calling activity, call composition, and acoustic parameters between treatments. Total call number was significantly higher in large choruses, and the increase was not directly proportional to the number of males present, rising by a factor of 4.2 when group size was tripled. The proportion of two-note calls also increased, with a large effect size, although this difference was not statistically significant after multiple-comparison correction at the present sample size. None of the four acoustic parameters (call duration, dominant frequency, note duration, and note interval) showed a detectable response to chorus size, a result confirmed by mixed models fitted to all 401 recorded calls. Dominant frequency was the most conservative parameter, consistent with morphological constraint and stabilizing selection by female choice. We also show that the coefficient of variation of call duration is inflated when calls differing in note number are pooled, indicating that this metric should instead be assessed within note-number classes. Our study provides the first quantitative description of the advertisement call of B. gargarizans and indicates that males respond to a crowded acoustic environment primarily by increasing calling effort rather than by adjusting the acoustic structure of individual calls. FAU - Hu, Hua-Li AU - Hu HL AD - College of Agriculture and Biotechnology, Lishui University, Lishui 323000, China. AD - College of Life Sciences, Nanjing Forestry University, Nanjing 210037, China. AD - Administration Center of Zhejiang Jiulongshan National Nature Reserve, Suichang, Lishui 323000, China. FAU - Chen, Zhi-Qiang AU - Chen ZQ AD - Yueqing Animal Disease Prevention and Control Center, Yueqing 325600, China. FAU - Li, Jing AU - Li J AD - Zhejiang Environmental Monitoring Engineering Co., Ltd., Hangzhou 311100, China. FAU - Zheng, Wei-Cheng AU - Zheng WC AD - Suichang County Ecological Forestry Development Center, Suichang, Lishui 323000, China. FAU - Ding, Guo-Hua AU - Ding GH AUID- ORCID: 0000-0002-2484-5855 AD - College of Agriculture and Biotechnology, Lishui University, Lishui 323000, China. LA - eng GR - 2026SNJF029/Department of Agriculture and Rural Affairs of Zhejiang Province/ PT - Journal Article DEP - 20261011 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700558 OTO - NOTNLM OT - Bufo gargarizans OT - acoustic communication OT - behavioral plasticity OT - call composition OT - chorus size OT - explosive breeding COIS- Jing Li is employed by Zhejiang Environmental Monitoring Engineering Co., Ltd. The company had no role in the study design; in the collection, analysis, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. All authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/11 CRDT- 2026/10/27 10:32 PHST- 2026/08/11 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - ani16203187 [pii] AID - animals-16-03187 [pii] AID - 10.3390/ani16203187 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 11;16(20):3187. doi: 10.3390/ani16203187. PMID- 42939116 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 10 TI - Clinical Reasoning in Veterinary Ophthalmology: A Pathophysiology-Centered Framework for Canine and Feline Clinical Practice. LID - 10.3390/ani16203185 [doi] LID - 3185 AB - Clinical reasoning enables veterinarians to make clinical decisions for individual patients by integrating clinical information with scientific evidence, knowledge of underlying disease processes, and case-specific circumstances. This review aimed to clarify clinical reasoning in canine and feline ophthalmology, with particular emphasis on pathophysiological interpretation, and to organize major theoretical frameworks of clinical reasoning in relation to the ophthalmic diagnostic process. A narrative and conceptual review was conducted using PubMed and Google Scholar, with literature searched from February 2026 through 31 August 2026. Relevant publications were selected according to their conceptual contribution to clinical reasoning, cognition, decision-making, clinical practice, or clinical education rather than predefined systematic-review eligibility criteria. Fifteen theoretical frameworks were organized into four groups according to their primary theoretical roles: historical and normative theories of reasoning; foundational theories of modern cognitive science; theories of clinical reasoning and decision-making; and theories of clinical education and expertise. The framework was illustrated through three representative clinical scenarios involving glaucoma in a Shiba Inu, a descemetocele with aqueous humor leakage in a Japanese cat, and traumatic proptosis in a Chihuahua. The analysis demonstrates how different theoretical perspectives can contribute to the interpretation of clinical information and its connection to pathophysiological understanding and clinical decision-making. PCCR is proposed as an integrative clinical reasoning framework that places pathophysiological interpretation between clinical information and patient-specific decision-making. Its applicability beyond veterinary ophthalmology remains a conceptual proposition requiring future evaluation. FAU - Kanemaki, Nobuyuki AU - Kanemaki N AUID- ORCID: 0000-0002-8736-6720 AD - School of Veterinary Medicine, Azabu University, 1-17-71 Fuchinobe, Chuo-ku, Sagamihara 252-5201, Kanagawa, Japan. AD - DVMs Animal Medical Center Yokohama, 2-2 Sawatari, Kanagawa-ku, Yokohama 221-0844, Kanagawa, Japan. LA - eng PT - Journal Article PT - Review DEP - 20261010 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700552 OTO - NOTNLM OT - cats OT - clinical decision-making OT - clinical reasoning OT - dogs OT - pathophysiology-centered reasoning OT - theoretical frameworks OT - veterinary ophthalmology COIS- The author declares no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:32 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - ani16203185 [pii] AID - animals-16-03185 [pii] AID - 10.3390/ani16203185 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 10;16(20):3185. doi: 10.3390/ani16203185. PMID- 42939114 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 10 TI - Adaptive Mechanisms of Endemic Fish Triplophysa yarkandensis in the Tarim River Basin of Northwestern China. LID - 10.3390/ani16203183 [doi] LID - 3183 AB - Dryland river ecosystems are among the most susceptible freshwater environments, with climate change, hydrological shifts, salinization and anthropogenic disturbance posing increasing threats to aquatic biodiversity. Endemic fishes in these regions are particularly vulnerable because of restricted geographic range, ecological specialization and narrow environmental tolerance limits, which reduce their capacity to cope with rapid environmental change. Triplophysa yarkandensis has a coordinated set of adaptive responses such as benthic morphological specialization, adaptable trophic modes, osmoregulatory ability to cope with saline-alkaline stress, and thermal tolerance mediated by stress-sensitive genes. Notably, muscle transcriptomic evidence indicates heat-associated expression changes in genes of the IRE1-TRAF2-MAP3K5-JNK signaling axis, suggesting this pathway as a candidate link between endoplasmic-reticulum stress and apoptosis-related responses. But its activation and causal role in apoptosis have not yet been confirmed at the protein and functional level. Nevertheless, these adaptations are increasingly constrained by habitat fragmentation, invasive species, declining water quality, and altered flow regimes, highlighting the limited buffering capacity of endemic species under increasing environmental disturbance. These observations provide a scientific basis for conservation planning, ecological monitoring, and adaptive management in arid inland waters, including habitat restoration, ecological flow regulation, and species-specific protection. Future studies must combine long-term field studies, multi-stressor experimental systems, and genomic-ecophysiological designs to define adaptive limits and improve conservation outcomes under continued climate change. FAU - Bibi, Sadia AU - Bibi S AD - College of Life Sciences and Technology, Key Laboratory of Tarim University, Tarim Research Center of Rare Fishes, Alar 843300, China. FAU - Zhu, Yafan AU - Zhu Y AUID- ORCID: 0000-0003-1252-6619 AD - College of Life Sciences and Technology, Key Laboratory of Tarim University, Tarim Research Center of Rare Fishes, Alar 843300, China. FAU - Liu, Changcai AU - Liu C AD - College of Life Sciences and Technology, Key Laboratory of Tarim University, Tarim Research Center of Rare Fishes, Alar 843300, China. FAU - Yang, Liting AU - Yang L AUID- ORCID: 0009-0004-7562-6590 AD - College of Life Sciences and Technology, Key Laboratory of Tarim University, Tarim Research Center of Rare Fishes, Alar 843300, China. FAU - Wang, Kai AU - Wang K AD - College of Life Sciences and Technology, Key Laboratory of Tarim University, Tarim Research Center of Rare Fishes, Alar 843300, China. FAU - Zhu, Aiai AU - Zhu A AD - College of Life Sciences and Technology, Key Laboratory of Tarim University, Tarim Research Center of Rare Fishes, Alar 843300, China. FAU - Yu, Liu AU - Yu L AD - College of Life Sciences and Technology, Key Laboratory of Tarim University, Tarim Research Center of Rare Fishes, Alar 843300, China. FAU - Song, Yong AU - Song Y AD - College of Life Sciences and Technology, Key Laboratory of Tarim University, Tarim Research Center of Rare Fishes, Alar 843300, China. FAU - Li, Hongmei AU - Li H AD - Shanghai Society of Oceanology and Limnology, East China Sea Ecology Center, Ministry of Natural Resources, Shanghai 200062, China. FAU - Huo, Bin AU - Huo B AD - College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. FAU - Chen, Shengao AU - Chen S AUID- ORCID: 0000-0003-0716-8511 AD - College of Life Sciences and Technology, Key Laboratory of Tarim University, Tarim Research Center of Rare Fishes, Alar 843300, China. LA - eng GR - 2025AB008/Xinjiang Production and Construction Corps/ GR - XARS-08-202614/Xinjiang Agriculture Research System/ GR - 2023TSYCCX0128/Tianshan Talent Training Project of Xinjiang/ PT - Journal Article PT - Review DEP - 20261010 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700832 OTO - NOTNLM OT - conservation biology OT - endemic fishes OT - salinity-alkalinity stress OT - thermal tolerance OT - transcriptomic analyses COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:32 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - ani16203183 [pii] AID - animals-16-03183 [pii] AID - 10.3390/ani16203183 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 10;16(20):3183. doi: 10.3390/ani16203183. PMID- 42939106 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 10 TI - Cortisol in Saliva, Urine, Feces, and Hair as Non- and Minimally Invasive Measures of Adrenocortical Activity in Intensively Housed Pigs. LID - 10.3390/ani16203175 [doi] LID - 3175 AB - Blood cortisol measurements may be confounded by sampling-induced handling and restraint, motivating the use of alternative matrices to assess adrenocortical activity in pigs. This narrative review examines the physiological time windows, collection requirements, analytical validation, and interpretive limitations of saliva, urine, feces, and hair. Saliva captures rapid responses but remains sensitive to collection conditions; urine integrates secretion over bladder filling and requires dilution correction; fecal glucocorticoid metabolites reflect delayed, integrated excretion and require a species-appropriate assay. Hair offers a potentially longer integration window, but controlled validation in pigs remains inconsistent, and clipping generally requires brief restraint. Antibody specificity, sample processing, and normalization constrain comparisons between studies. Published concentration benchmarks should not be interpreted as validated, production-stage-specific reference intervals. The practical matrix-selection framework presented here rests primarily on cross-study inference rather than direct comparisons of all four matrices under a common challenge. Cortisol indicates adrenocortical activation without establishing its cause or emotional valence. Interpretation therefore requires complementary behavioral, clinical, and production measures. A minimum reporting standard and priorities for paired-matrix validation, standardized sampling, and category-specific reference intervals are proposed. These matrices support comparative monitoring; none should be treated as a standalone diagnostic test of compromised welfare. FAU - Kovacs, Levente AU - Kovacs L AD - Department of Animal Husbandry and Animal Welfare, Institute of Animal Sciences, Hungarian University of Agriculture and Life Sciences, Pater Karoly utca 1, H-2100 Godollo, Hungary. FAU - Orban, Marton AU - Orban M AD - Vitafort First Hungarian Feed Production and Distribution Zrt., Szabadsag u. 3, H-2370 Dabas, Hungary. FAU - Gregosits, Balazs AU - Gregosits B AD - Vitafort First Hungarian Feed Production and Distribution Zrt., Szabadsag u. 3, H-2370 Dabas, Hungary. FAU - Erdelyi, Marta AU - Erdelyi M AUID- ORCID: 0000-0003-2928-3545 AD - Department of Feed Safety, Institute of Animal Physiology and Nutrition, Hungarian University of Agriculture and Life Sciences, Pater Karoly utca 1, H-2100 Godollo, Hungary. FAU - Mezes, Miklos AU - Mezes M AUID- ORCID: 0000-0003-2323-833X AD - Department of Feed Safety, Institute of Animal Physiology and Nutrition, Hungarian University of Agriculture and Life Sciences, Pater Karoly utca 1, H-2100 Godollo, Hungary. LA - eng GR - 2023-1.1.1-PIACI FOKUSZ 2024-00050/National Research, Development and Innovation Office/ PT - Journal Article PT - Review DEP - 20261010 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700866 OTO - NOTNLM OT - fecal glucocorticoid metabolites OT - hair cortisol OT - hypothalamic-pituitary-adrenal axis OT - non- and minimally invasive sampling OT - salivary cortisol OT - sow OT - stress OT - urinary cortisol OT - welfare assessment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:32 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/06 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - ani16203175 [pii] AID - animals-16-03175 [pii] AID - 10.3390/ani16203175 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 10;16(20):3175. doi: 10.3390/ani16203175. PMID- 42939100 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2076-2615 (Print) IS - 2076-2615 (Electronic) IS - 2076-2615 (Linking) VI - 16 IP - 20 DP - 2026 Oct 9 TI - Milk Production and Climate Variability in a High-Andean Dairy System: A Longitudinal Case Study. LID - 10.3390/ani16203169 [doi] LID - 3169 AB - Climatic variability can modify milk production through direct and indirect pathways, yet monthly farm time series also exhibit temporal dependence. A longitudinal, observational, single-case study was conducted in the San Francisco dairy system, Carchi, Ecuador, from March 2019 to December 2024. Of 70 calendar months, 64 had complete analytical information, and 58 were eligible for two-lag models. Ten model specifications were compared based on model fit, information criteria, residual diagnostics, seasonality assessment, and temporal validation. Mean daily milk production was 233.49 +/- 47.90 L day(-1). The selected model was a second-order autoregressive model with a calendar trend and mean temperature at t - 2 (adjusted R2 = 0.827; Akaike information criterion = 519.52; Bayesian information criterion = 529.83; root mean squared error = 19.56 L day(-1)). The temperature coefficient was 10.844 L day(-1) degrees C(-1) (p < 0.001 with robust standard errors). Annual harmonic terms did not improve model fit, and precipitation at t - 1 yielded only marginal improvement. The number of cows in production varied across 54 months (26.02 +/- 4.11; range: 18-37). The observed temperature association should be interpreted at the system level; it does not demonstrate direct causality or individual cow productivity and requires external validation. FAU - Montenegro-Arellano, Guillermo Fausto AU - Montenegro-Arellano GF AD - Universidad Politecnica Estatal del Carchi, Antisana St. and Universitaria, Ave., Tulcan 040102, Carchi, Ecuador. FAU - Carvajal-Perez, Luis Alfredo AU - Carvajal-Perez LA AUID- ORCID: 0000-0002-3039-7657 AD - Universidad Politecnica Estatal del Carchi, Antisana St. and Universitaria, Ave., Tulcan 040102, Carchi, Ecuador. FAU - Urgiles-Urgiles, Gladys Primavera AU - Urgiles-Urgiles GP AD - Universidad Politecnica Estatal del Carchi, Antisana St. and Universitaria, Ave., Tulcan 040102, Carchi, Ecuador. FAU - Quishpe-Sinailin, Pablo David AU - Quishpe-Sinailin PD AD - Instituto de Investigaciones Economicas, Universidad Central del Ecuador, Quito 170129, Pichincha, Ecuador. FAU - Herrera-Lana, Anibal Eduardo AU - Herrera-Lana AE AD - Instituto de Investigaciones Economicas, Universidad Central del Ecuador, Quito 170129, Pichincha, Ecuador. LA - eng GR - Research Project DIIN-2026-11/Universidad Politecnica Estatal del Carchi/ PT - Journal Article DEP - 20261009 PL - Switzerland TA - Animals (Basel) JT - Animals : an open access journal from MDPI JID - 101635614 PMC - PMC13700705 OTO - NOTNLM OT - autocorrelation OT - climate variability OT - dairy cattle OT - high-Andean system OT - milk production OT - single-case study OT - temperature OT - time series COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/09 CRDT- 2026/10/27 10:32 PHST- 2026/09/10 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/09 00:00 [pmc-release] AID - ani16203169 [pii] AID - animals-16-03169 [pii] AID - 10.3390/ani16203169 [doi] PST - epublish SO - Animals (Basel). 2026 Oct 9;16(20):3169. doi: 10.3390/ani16203169. PMID- 42939090 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 21 TI - Bridging the Gap Between Experimental and Clinical Applications of Mesenchymal Stromal Cell Therapy in Intervertebral Disc Regeneration. LID - 10.3390/bioengineering13101234 [doi] LID - 1234 AB - Intervertebral disc (IVD) degeneration is a major cause of chronic low back pain and disability worldwide. Although current treatment strategies effectively alleviate symptoms, they do not restore disc structure or reverse the underlying biological processes responsible for degeneration. Mesenchymal stromal cell (MSC)-based therapies have emerged as promising regenerative approaches owing to their immunomodulatory, anti-inflammatory, and extracellular matrix-regenerative properties, mediated predominantly through paracrine signalling rather than long-term cellular engraftment. Despite encouraging preclinical results, clinical translation remains limited by poor cell survival within the hostile disc microenvironment, protocol heterogeneity, and insufficient long-term clinical evidence. This review critically examines the current state of MSC-based therapy for IVD degeneration by integrating experimental findings, clinical evidence, and emerging translational strategies. Particular emphasis is placed on the biological mechanisms of MSC-mediated regeneration, comparison of different MSC sources, advances in extracellular vesicle- and exosome-based cell-free therapies, and the growing role of quantitative magnetic resonance imaging, molecular biomarkers, and precision diagnostics in patient selection and treatment monitoring. In addition, the review discusses the major biological, technical, manufacturing, and regulatory challenges that currently limit clinical implementation. Collectively, the evidence indicates that successful translation of MSC-based therapies will require not only optimised cell products but also standardised manufacturing, biomaterial-assisted delivery systems, advanced diagnostic biomarkers, and well-designed multicentre clinical trials. Integrating these advances will be essential for transforming MSC-based therapies into safe, standardised, and disease-modifying treatments for patients with intervertebral disc degeneration. FAU - Gradisnik, Lidija AU - Gradisnik L AUID- ORCID: 0000-0003-3492-0297 AD - Institute of Biomedical Sciences, Medical Faculty of Maribor, 2000 Maribor, Slovenia. FAU - Prestor, Borut AU - Prestor B AD - Department of Neurosurgery, University Medical Centre Ljubljana, 1000 Ljubljana, Slovenia. AD - Medical Faculty of Ljubljana, 1000 Ljubljana, Slovenia. FAU - Munda, Matic AU - Munda M AD - Department of Neurosurgery, University Medical Centre Ljubljana, 1000 Ljubljana, Slovenia. FAU - Zele, Tilen AU - Zele T AD - Department of Neurosurgery, University Medical Centre Ljubljana, 1000 Ljubljana, Slovenia. AD - Medical Faculty of Ljubljana, 1000 Ljubljana, Slovenia. AD - Alma Mater Europaea University Maribor, 2000 Maribor, Slovenia. FAU - Maver, Uros AU - Maver U AUID- ORCID: 0000-0002-2237-3786 AD - Institute of Biomedical Sciences, Medical Faculty of Maribor, 2000 Maribor, Slovenia. FAU - Kocivnik, Nina AU - Kocivnik N AD - Institute of Biomedical Sciences, Medical Faculty of Maribor, 2000 Maribor, Slovenia. FAU - Velnar, Tomaz AU - Velnar T AUID- ORCID: 0000-0002-6283-4348 AD - Department of Neurosurgery, University Medical Centre Ljubljana, 1000 Ljubljana, Slovenia. AD - Medical Faculty of Ljubljana, 1000 Ljubljana, Slovenia. AD - Alma Mater Europaea University Maribor, 2000 Maribor, Slovenia. LA - eng GR - J3-60058/The Slovenian Research and Innovation Agency/ PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701582 OTO - NOTNLM OT - biomarkers OT - intervertebral disc OT - mesenchymal stromal cells OT - regenerative medicine OT - tissue engineering COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/21 CRDT- 2026/10/27 10:32 PHST- 2026/09/20 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - bioengineering13101234 [pii] AID - bioengineering-13-01234 [pii] AID - 10.3390/bioengineering13101234 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 21;13(10):1234. doi: 10.3390/bioengineering13101234. PMID- 42939089 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 21 TI - International Regulatory Classification of Minimally Manipulated and Borderline Cell and Tissue Products: Implications for Mesenchymal Stromal Cell-Based Interventions. LID - 10.3390/bioengineering13101235 [doi] LID - 1235 AB - Cell and tissue products used in regenerative medicine are classified differently across jurisdictions according to processing, intended use, and manufacturing setting. This review compares regulatory approaches to products including bone marrow aspirate concentrate, adipose-derived stromal vascular fraction (SVF), microfragmented adipose tissue, nanofat, amniotic products, and cartilage-derived products, with particular attention to their implications for mesenchymal stromal cell (MSC)-based interventions. Legislation, regulatory guidance, enforcement documents, judicial decisions, and scientific publications were examined for the United States, European Union, Russian Federation, Eurasian Economic Union, selected Asian countries, and Arab jurisdictions. In the United States, minimal manipulation and homologous use are central classification criteria. In the European Union, substantial manipulation or use for a different essential function may bring a product within the advanced therapy medicinal product framework. Russian and Eurasian rules require distinction among biomedical cell products, individualized products, medicinal products, transplantation materials, and medical procedures. SVF is a heterogeneous cell population, while microfragmented adipose tissue retains tissue structures. Neither should be equated with an isolated or culture-expanded MSC preparation solely because it contains stromal cells. Regulatory assessment and interpretation of clinical evidence require clear reporting of product composition, processing, claimed mechanism of action, and manufacturing conditions. FAU - Bilyalov, Airat AU - Bilyalov A AUID- ORCID: 0000-0002-8888-8395 AD - Petrovsky National Research Centre of Surgery, Moscow 117418, Russia. FAU - Filatov, Nikita AU - Filatov N AUID- ORCID: 0009-0000-9179-515X AD - Petrovsky National Research Centre of Surgery, Moscow 117418, Russia. FAU - Iashina, Natalia AU - Iashina N AD - Petrovsky National Research Centre of Surgery, Moscow 117418, Russia. FAU - Briko, Andrey AU - Briko A AUID- ORCID: 0000-0002-7284-5649 AD - Petrovsky National Research Centre of Surgery, Moscow 117418, Russia. FAU - Chibizov, Pavel AU - Chibizov P AD - Petrovsky National Research Centre of Surgery, Moscow 117418, Russia. FAU - Nadelyaeva, Irina AU - Nadelyaeva I AD - Petrovsky National Research Centre of Surgery, Moscow 117418, Russia. FAU - Tikhomirov, Alexey AU - Tikhomirov A AUID- ORCID: 0000-0002-5989-9044 AD - Petrovsky National Research Centre of Surgery, Moscow 117418, Russia. FAU - Petrikina, Anastasia AU - Petrikina A AD - Petrovsky National Research Centre of Surgery, Moscow 117418, Russia. FAU - Vasilyev, Viatcheslav AU - Vasilyev V AUID- ORCID: 0000-0002-2220-9695 AD - Kazan State Medical Academy, Branch Campus of the Russian Medical Academy of Continuous Professional Education, Ministry of Health of the Russian Federation, Kazan 420012, Russia. FAU - Deev, Roman AU - Deev R AUID- ORCID: 0000-0001-8389-3841 AD - Petrovsky National Research Centre of Surgery, Moscow 117418, Russia. FAU - Eremin, Ilya AU - Eremin I AD - Petrovsky National Research Centre of Surgery, Moscow 117418, Russia. FAU - Kotenko, Konstantin AU - Kotenko K AD - Petrovsky National Research Centre of Surgery, Moscow 117418, Russia. LA - eng GR - FURG-2025-0028/The Ministry of Education and Science of the Russian Federation/ PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701192 OTO - NOTNLM OT - advanced therapy medicinal products OT - biomedical cell products OT - cell therapy OT - homologous use OT - mesenchymal stromal cells OT - minimally manipulated products OT - regenerative medicine OT - regulatory framework OT - stromal vascular fraction OT - substantial manipulation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/21 CRDT- 2026/10/27 10:32 PHST- 2026/09/24 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - bioengineering13101235 [pii] AID - bioengineering-13-01235 [pii] AID - 10.3390/bioengineering13101235 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 21;13(10):1235. doi: 10.3390/bioengineering13101235. PMID- 42939088 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 21 TI - Criterion Validity of Wrist-Worn IMU-Derived Smoothness Metrics During Functional Upper-Limb Tasks. LID - 10.3390/bioengineering13101233 [doi] LID - 1233 AB - Wearable inertial measurement units (IMUs) may facilitate quantitative assessment of upper-limb motor function outside laboratory settings; however, the validity of IMU-derived movement smoothness metrics compared with optical motion capture remains unclear. This study aimed to investigate the criterion validity of wrist-worn IMU-derived smoothness metrics during functional upper-limb tasks. Twenty-seven healthy adults (14 females, 13 males) performed two functional tasks, Reaching and Hand-to-mouth, with both upper limbs. Movements were simultaneously recorded using a marker-based motion capture system and two wrist-worn IMUs, both sampling at 100 Hz. Acceleration profiles were obtained from MoCap-derived wrist trajectories and IMU signals, and four smoothness metrics were calculated: Spectral Arc Length (SPARC), normalized root mean square (nRMS), Normalized Jerk (NJ), and Log Dimensionless Jerk (LDLJ). Criterion validity was assessed through Pearson correlation, estimation error (RMSE and MAE), Bland-Altman analysis, and proportional bias assessment. During the Reaching task, IMU-derived metrics showed significant correlations with MoCap values for all indices (r = 0.41-0.74), with NJ showing the strongest association. SPARC showed the smallest mean bias between systems. During the Hand-to-mouth task, significant correlations were observed only for SPARC and NJ (r = 0.39-0.47), with increased discrepancies for nRMS and LDLJ. Wrist-worn IMUs provide comparable smoothness estimates to MoCap during specific upper-limb tasks, but agreement depends on both the selected metric and movement characteristics. These findings highlight the need for task- and metric-specific validation before translating laboratory-based movement quality assessment into wearable settings. FAU - Cerfoglio, Serena AU - Cerfoglio S AUID- ORCID: 0000-0002-1983-8204 AD - Department of Electronics, Information and Bioengineering, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy. AD - IRCCS Istituto Auxologico Italiano, San Giuseppe Hospital, Strada Luigi Cadorna 90, 28824 Piancavallo, Italy. FAU - Donno, Lucia AU - Donno L AUID- ORCID: 0000-0003-4297-2433 AD - Department of Electronics, Information and Bioengineering, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy. AD - E4Sport-Human Performance Lab, Polo Territoriale di Lecco, Politecnico di Milano, Via G. Previati 1/c, 23900 Lecco, Italy. FAU - Lovato, Mattia AU - Lovato M AUID- ORCID: 0009-0004-9248-5603 AD - Department of Electronics, Information and Bioengineering, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy. AD - E4Sport-Human Performance Lab, Polo Territoriale di Lecco, Politecnico di Milano, Via G. Previati 1/c, 23900 Lecco, Italy. FAU - Francia, Carlalberto AU - Francia C AUID- ORCID: 0009-0009-9454-7181 AD - Department of Electronics, Information and Bioengineering, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy. AD - E4Sport-Human Performance Lab, Polo Territoriale di Lecco, Politecnico di Milano, Via G. Previati 1/c, 23900 Lecco, Italy. FAU - Galli, Manuela AU - Galli M AUID- ORCID: 0000-0003-2772-4837 AD - Department of Electronics, Information and Bioengineering, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy. AD - E4Sport-Human Performance Lab, Polo Territoriale di Lecco, Politecnico di Milano, Via G. Previati 1/c, 23900 Lecco, Italy. FAU - Cimolin, Veronica AU - Cimolin V AUID- ORCID: 0000-0001-6299-7254 AD - Department of Electronics, Information and Bioengineering, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy. AD - IRCCS Istituto Auxologico Italiano, San Giuseppe Hospital, Strada Luigi Cadorna 90, 28824 Piancavallo, Italy. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701579 OTO - NOTNLM OT - criterion validity OT - functional motor tasks OT - inertial measurement units OT - movement smoothness OT - optical motion capture OT - upper-limb kinematics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/21 CRDT- 2026/10/27 10:32 PHST- 2026/09/25 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - bioengineering13101233 [pii] AID - bioengineering-13-01233 [pii] AID - 10.3390/bioengineering13101233 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 21;13(10):1233. doi: 10.3390/bioengineering13101233. PMID- 42939085 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 21 TI - Hemolysis During Ex Vivo Lung Perfusion Is Associated with Endothelial Injury, Cytokine Release, and Primary Graft Dysfunction. LID - 10.3390/bioengineering13101230 [doi] LID - 1230 AB - Primary graft dysfunction (PGD) is a major cause of early morbidity and mortality after lung transplantation. During blood-based ex vivo lung perfusion (EVLP), hemolysis releases plasma-free hemoglobin (pfHb) and other erythrocyte-derived products that may promote endothelial injury and inflammation. We investigated whether EVLP-associated hemolysis is associated with cytokine release, endothelial dysfunction, and grade 3 PGD (PGD3). In this prospective, single-center observational study, end-EVLP perfusate from 57 double-lung transplant recipients supported with the Organ Care System Lung was analyzed for pfHb, 26 cytokines, blood counts, and associations with PGD3 within 72 h after transplantation. A mock EVLP blood-loop model was used to evaluate the effects of red blood cell storage duration and circuit run time on hemolysis. Generated hemolytic perfusates were applied to human lung microvascular endothelial cells to assess barrier injury and inflammatory activation. Among the 57 recipients, 32 (56.1%) developed PGD3 at any time point within 72 h after transplantation. Higher end-EVLP pfHb, interleukin-6, and interleukin-8 levels were associated with PGD3, and pfHb correlated positively with interleukin-6 and interleukin-8. Hemolysis increased with longer blood storage and EVLP duration, with storage duration emerging as the dominant contributor in the blood-loop model. Hemolytic perfusate disrupted endothelial junctions, increased permeability, and induced interleukin-6 and interleukin-8 release. EVLP-associated hemolysis is linked to endothelial barrier injury, inflammatory activation, and PGD3 after lung transplantation. Reducing hemolysis through improved blood-product selection and EVLP circuit management may represent a modifiable strategy to improve posttransplant outcomes. FAU - Chan, Chris H H AU - Chan CHH AUID- ORCID: 0000-0001-7983-5614 AD - Innovative Device & Engineering Applications Lab, The Texas Heart Institute, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Kanchi, Rupa AU - Kanchi R AUID- ORCID: 0009-0006-2571-7077 AD - Advanced Technology Cores, Dan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Mesquita, Fernanda C P AU - Mesquita FCP AUID- ORCID: 0000-0003-1038-010X AD - Regenerative Medicine Research Lab, The Texas Heart Institute, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Lopez, Patricia Da Costa AU - Lopez PDC AD - Advanced Technology Cores, Dan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Wang, Yaxin AU - Wang Y AD - Innovative Device & Engineering Applications Lab, The Texas Heart Institute, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Farooqui, Asma F AU - Farooqui AF AD - Innovative Device & Engineering Applications Lab, The Texas Heart Institute, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Lim, Wilson AU - Lim W AD - Innovative Device & Engineering Applications Lab, The Texas Heart Institute, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Leon Pena, Andres AU - Leon Pena A AUID- ORCID: 0000-0002-4010-9794 AD - Division of Cardiopulmonary Transplantation and Mechanical Circulatory Support, Michael E. DeBakey Department of Surgery, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Hills, Emma E AU - Hills EE AUID- ORCID: 0000-0001-9256-9429 AD - Division of Cardiopulmonary Transplantation and Mechanical Circulatory Support, Michael E. DeBakey Department of Surgery, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Coarfa, Cristian AU - Coarfa C AD - Advanced Technology Cores, Dan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Elsenousi, Abdussalam AU - Elsenousi A AD - Division of Cardiopulmonary Transplantation and Mechanical Circulatory Support, Michael E. DeBakey Department of Surgery, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Mattar, Aladdein AU - Mattar A AD - Division of Cardiopulmonary Transplantation and Mechanical Circulatory Support, Michael E. DeBakey Department of Surgery, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Sharma, Nirmal S AU - Sharma NS AD - Division of Cardiopulmonary Transplantation and Mechanical Circulatory Support, Michael E. DeBakey Department of Surgery, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Fernandez, Ramiro AU - Fernandez R AD - Division of Cardiopulmonary Transplantation and Mechanical Circulatory Support, Michael E. DeBakey Department of Surgery, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Hochman-Mendez, Camila AU - Hochman-Mendez C AD - Advanced Technology Cores, Dan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, TX 77030, USA. AD - Regenerative Medicine Research Lab, The Texas Heart Institute, Baylor College of Medicine, Houston, TX 77030, USA. FAU - Loor, Gabriel AU - Loor G AUID- ORCID: 0000-0002-7275-2434 AD - Division of Cardiopulmonary Transplantation and Mechanical Circulatory Support, Michael E. DeBakey Department of Surgery, Baylor College of Medicine, Houston, TX 77030, USA. LA - eng GR - T32 HL139430/HL/NHLBI NIH HHS/United States GR - 24RDM001/The Roderick D. MacDonald Research Fund at Baylor St. Luke's Medical Center/ GR - 10004413/JLH Foundation/ GR - 3670046125111/Hedleston Heart Transplant Fund/ GR - 25RDM003/The Roderick D. MacDonald Research Fund at Baylor St. Luke's Medical Center/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701639 OTO - NOTNLM OT - cytokine release OT - endothelial injury OT - ex vivo lung perfusion OT - hemolysis OT - primary graft dysfunction COIS- Drs Chan, Wang, and Loor are cofounders of OrganVive, LLC. The remaining authors report no relationships with a commercial entity that has an interest in the subject matter of this manuscript, and they have no other conflicts of interest to disclose. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/21 CRDT- 2026/10/27 10:32 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - bioengineering13101230 [pii] AID - bioengineering-13-01230 [pii] AID - 10.3390/bioengineering13101230 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 21;13(10):1230. doi: 10.3390/bioengineering13101230. PMID- 42939084 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 21 TI - Nested Evaluation of Sparse Multi-Objective Evolutionary Gene-Panel Discovery for Diabetic Nephropathy. LID - 10.3390/bioengineering13101229 [doi] LID - 1229 AB - Background: Diabetic nephropathy gene panels come from small kidney transcriptome cohorts, where screening and adaptive search inflate performance. Evolutionary multi-objective search balances panel size, accuracy, and biological coherence, but dense initialization slows it, and its benefit over ranking or regularized regression is rarely tested under nested evaluation. Methods: Six evolutionary algorithms optimizing leanness, inner balanced accuracy, and Gene Ontology (GO) coherence were evaluated with four repeats of nested five-fold cross-validation on GSE96804. Harmonic initialization was compared with Bernoulli, bounded uniform, and SparseEA initializers; GO was removed or randomized. Comparators were random, top-ranked differentially expressed gene (top-DEG), regularized, and wrapper panels and a permuted-label null. Panels were transferred to GSE30528 and GSE142025. Results: Panels contained 2.10-2.70 genes. Harmonic initialization improved hypervolume over Bernoulli and matched existing sparse initializers. Outer area under the curve (AUC) was 0.973-0.991, comparable to top-DEG and regularized selectors; permuted labels gave 0.510. Real GO raised maximum archive coherence from 0.044 to 0.960, and a GO-aware rule raised representative coherence from 0.098 to 0.620 at equal AUC. External AUC was 0.612-0.726 and 0.849-0.964. Conclusions: Harmonic initialization yields compact Pareto fronts without a size cap, GO guidance supplies coherent panels at equal discrimination, and regularized selection gives the most reproducible panel. FAU - Cong, Yilei AU - Cong Y AD - Department of Endocrinology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. FAU - Wu, Tengfei AU - Wu T AD - Department of Endocrinology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. FAU - Yang, Xinyu AU - Yang X AD - Department of Endocrinology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. FAU - Zhu, Yiyun AU - Zhu Y AD - Ward 2, Department of Nephrology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. FAU - Tang, Min AU - Tang M AD - Department of Endocrinology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. FAU - Yang, Hua AU - Yang H AUID- ORCID: 0000-0002-7035-9911 AD - Department of Endocrinology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. FAU - Liu, Ping AU - Liu P AD - Department of Cardiology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. LA - eng GR - 25ZR1401334/Shanghai Science and Technology Commission/ GR - 20254Y0135/Shanghai Health Commission/ GR - 2026QN113/Shanghai Health Commission/ GR - CXZH2024057/China Science and Technology Development Center for Traditional Chinese Medicine/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701386 OTO - NOTNLM OT - diabetic nephropathy OT - feature selection OT - gene expression profiling OT - multi-objective optimization OT - nested cross-validation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/21 CRDT- 2026/10/27 10:32 PHST- 2026/09/22 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - bioengineering13101229 [pii] AID - bioengineering-13-01229 [pii] AID - 10.3390/bioengineering13101229 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 21;13(10):1229. doi: 10.3390/bioengineering13101229. PMID- 42939082 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 20 TI - Use of Bioimpedance Monitoring and Artificial Intelligence in Heart Failure: A Scoping Review. LID - 10.3390/bioengineering13101227 [doi] LID - 1227 AB - Heart failure (HF) is frequently accompanied by congestion and fluid accumulation, which are key drivers of decompensation and hospitalization. Bioimpedance wearable sensors (BI), coupled with artificial intelligence (AI) and clinical decision-support systems (CDSS), offer an emerging opportunity to move HF volume-status assessment beyond conventional clinical monitoring towards earlier risk detection and personalized decision support. This scoping review mapped the available evidence on these integrated approaches. Following PRISMA-ScR guidance, systematic searches were conducted in Scopus, IEEE Xplore, PubMed, and Embase, with the final search performed on August 2, 2026. Ten studies were included, of which six applied AI techniques, two incorporated a CDSS, and two included both AI and CDSS. BI measurements were acquired using heterogeneous approaches, including implanted devices, wearables, and other non-invasive systems, and they encompassed intrathoracic impedance, thoracic BI, or whole-body bioimpedance analysis. AI applications focused on HF decompensation, fluid accumulation, pleural effusion, and the risks of hospitalization or readmission, whereas CDSS primarily provided risk information, alerts, or structured clinical decision support. Overall, the integration of BI sensors with AI or CDSS represents a rapidly developing transformative approach to HF volume-status management, with the potential to enable earlier intervention and more individualized clinical decision-making. FAU - Puertas-Albarracin, Martin AU - Puertas-Albarracin M AD - Departamento de Tecnologia Electronica, Universidad de Sevilla, 41012 Sevilla, Spain. FAU - Gimenez-Miranda, Luis AU - Gimenez-Miranda L AUID- ORCID: 0000-0003-2588-5557 AD - Instituto de Biomedicina de Sevilla (IBiS-US), 41013 Sevilla, Spain. AD - Hospital Universitario Virgen del Rocio (HUVR), 41013 Sevilla, Spain. FAU - Medrano, Javier AU - Medrano J AUID- ORCID: 0000-0001-5863-344X AD - Instituto de Biomedicina de Sevilla (IBiS-US), 41013 Sevilla, Spain. AD - Hospital Universitario Virgen del Rocio (HUVR), 41013 Sevilla, Spain. AD - Consorcio de Investigacion Biomedica en Red de Epidemiologia y Salud Publica (CIBERESP), 41013 Sevilla, Spain. AD - Departamento de Medicina, Universidad de Sevilla, 41004 Sevilla, Spain. FAU - Romero-Ternero, Maria Del Carmen AU - Romero-Ternero MDC AUID- ORCID: 0000-0001-6965-9485 AD - Departamento de Tecnologia Electronica, Universidad de Sevilla, 41012 Sevilla, Spain. AD - Instituto de Biomedicina de Sevilla (IBiS-US), 41013 Sevilla, Spain. AD - Instituto de Ingenieria Informatica (I3US), Universidad de Sevilla, 41012 Sevilla, Spain. FAU - Oprescu, Andreea M AU - Oprescu AM AUID- ORCID: 0000-0003-3308-4688 AD - Departamento de Tecnologia Electronica, Universidad de Sevilla, 41012 Sevilla, Spain. AD - Instituto de Ingenieria Informatica (I3US), Universidad de Sevilla, 41012 Sevilla, Spain. FAU - Olmo, Alberto AU - Olmo A AUID- ORCID: 0000-0001-6388-4462 AD - Departamento de Tecnologia Electronica, Universidad de Sevilla, 41012 Sevilla, Spain. AD - Instituto de Microelectronica de Sevilla (IMSE-CNM-CSIC), 41092 Sevilla, Spain. LA - eng GR - USECHIP (TSI-069100-2023-001)/Secretary of State for Telecommunications and Digital Infraestructure, Ministry for Digital Transformation and Civil Service and by the European Union-NextGeneration EU/PRTR/ GR - PID2022-141045OBC41, C42,C43/MCIN/AEI /10.13039/ 501100011033/ and FEDER A way of making Europe/ PT - Journal Article PT - Review DEP - 20261020 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701678 OTO - NOTNLM OT - artificial intelligence OT - bioimpedance sensors OT - clinical decision support systems OT - congestion OT - heart failure OT - remote monitoring OT - volume status COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/20 CRDT- 2026/10/27 10:32 PHST- 2026/09/20 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - bioengineering13101227 [pii] AID - bioengineering-13-01227 [pii] AID - 10.3390/bioengineering13101227 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 20;13(10):1227. doi: 10.3390/bioengineering13101227. PMID- 42939079 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 19 TI - Brain-Computer Interfaces for Cognitive Rehabilitation: A Narrative Review. LID - 10.3390/bioengineering13101224 [doi] LID - 1224 AB - Cognitive diseases/disorders are a major cause of disability affecting millions of people worldwide due to pathologies or conditions such as Alzheimer's disease, Parkinson's disease, vascular cognitive impairment, and attention deficit/hyperactivity disorder. The effects of these diseases/disorders can range from subjective cognitive complaints to mild cognitive impairment and even dementia that can affect multiple cognitive domains, causing disability and alterations in daily functioning and quality of life. Cognitive training has been researched as a potential therapeutic option for treating cognitive disability. In particular, cognitive training delivered using brain-computer interfaces (BCIs) represents a promising technology for cognitive rehabilitation. For this reason, this work presents a narrative review aimed at bridging the clinical aspects of cognition performance and the technical features of BCI systems. Additionally, it reports on the current advances of BCIs for cognitive rehabilitation in the pathologies/conditions that are the main contributors of cognitive impairment globally. Specifically, Alzheimer's disease, Parkinson's disease, mild cognitive impairment, cerebrovascular diseases, and attention-deficit/hyperactivity disorder were included in the review; other pathologies that can also cause cognitive impairment such as multiple sclerosis, traumatic brain injury, brain tumors, infectious diseases, and psychiatric conditions such as schizophrenia or autism spectrum disorder, or drug or toxin-related causes were outside the scope of the present review. Across the literature, studies have reported that BCI cognitive training can improve cognition performance for mild cognitive impairment, stroke, and attention-deficit/hyperactivity disorder, showcasing its feasibility as a complementary therapy. However, the amount of evidence is still scarce, with few reported randomized controlled clinical trials and all of the applications comprising non-invasive systems using electroencephalography, while invasive or other non-invasive approaches remain largely unassessed. Therefore, further research regarding the design of BCIs for targeting specific cognitive domains is necessary to allow for the future introduction of these types of systems into clinical rehabilitation environments, rather than only demonstrating their feasibility, as has been mainly done to date. FAU - Cantillo-Negrete, Jessica AU - Cantillo-Negrete J AD - Technological Research Subdirection, Instituto Nacional de Rehabilitacion Luis Guillermo Ibarra Ibarra, Mexico City 14389, Mexico. FAU - Rodriguez-Garcia, Martin Emiliano AU - Rodriguez-Garcia ME AD - Division of Research in Clinical Neurosciences, Instituto Nacional de Rehabilitacion Luis Guillermo Ibarra Ibarra, Mexico City 14389, Mexico. FAU - Carrillo-Mora, Paul AU - Carrillo-Mora P AD - Division of Research in Clinical Neurosciences, Instituto Nacional de Rehabilitacion Luis Guillermo Ibarra Ibarra, Mexico City 14389, Mexico. FAU - Mendez-Gomez, Dalia Danely AU - Mendez-Gomez DD AD - Division of Research in Clinical Neurosciences, Instituto Nacional de Rehabilitacion Luis Guillermo Ibarra Ibarra, Mexico City 14389, Mexico. FAU - Carino-Escobar, Ruben I AU - Carino-Escobar RI AUID- ORCID: 0000-0001-7075-413X AD - Bioelectronics Section, Department of Electrical Engineering, Centro de Investigacion y de Estudios Avanzados del Instituto Politecnico Nacional, Mexico City 07360, Mexico. LA - eng PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701623 OTO - NOTNLM OT - ADHD OT - Alzheimer's disease OT - BCI OT - EEG OT - MCI OT - Parkinson's disease OT - dementia OT - stroke COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/19 CRDT- 2026/10/27 10:32 PHST- 2026/08/12 00:00 [received] PHST- 2026/09/26 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - bioengineering13101224 [pii] AID - bioengineering-13-01224 [pii] AID - 10.3390/bioengineering13101224 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 19;13(10):1224. doi: 10.3390/bioengineering13101224. PMID- 42939077 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 19 TI - A Knowledge Transfer Method from Multi-Channel to Single-Channel EEG Toward Real-World Emotion Recognition Deployment. LID - 10.3390/bioengineering13101223 [doi] LID - 1223 AB - Multi-channel electroencephalography (EEG) provides spatial information for emotion recognition but requires more complex signal acquisition than single-channel EEG. This study proposes a multi-channel-to-single-channel knowledge transfer framework that uses multi-channel recordings during training and one selected physical EEG channel at inference. A 32-channel teacher is pretrained, and Sparsemax-based gating is jointly optimized with a student using classification, logits distillation, frequency-band consistency, and gating regularization losses. The frequency-band objective compares signal-level spectral statistics of the multi-channel recordings and the gated surrogate input. After channel locking, the student is retrained on the selected physical signal. Binary Arousal and Valence classification was evaluated on DEAP using 32-fold leave-one-subject-out cross-validation. The proposed achieved higher subject-averaged balanced accuracy and F1-score than Channel-matched CE in both tasks, although component effects varied across tasks and metrics. These findings support the evaluated training approach under single-channel input constraints and provide a methodological basis for simplifying EEG acquisition. FAU - Gu, Yanru AU - Gu Y AD - School of Management, Fudan University, Shanghai 200433, China. AD - Teaching and Research Department, Shanghai Institute of Science and Technology Management, Shanghai 201800, China. FAU - Ouyang, Hanzhen AU - Ouyang H AD - School of Management, Fudan University, Shanghai 200433, China. FAU - Ouyang, Zihua AU - Ouyang Z AD - School of Management, Fudan University, Shanghai 200433, China. FAU - Yue, Lishengsa AU - Yue L AD - College of Transportation, Tongji University, Shanghai 201804, China. FAU - Mao, Huajuan AU - Mao H AD - The First Affiliated Hospital of Naval Medical University, Shanghai 200433, China. FAU - Dai, Weihui AU - Dai W AUID- ORCID: 0000-0002-9426-5303 AD - School of Management, Fudan University, Shanghai 200433, China. FAU - Kang, Yan AU - Kang Y AUID- ORCID: 0009-0005-6408-1246 AD - School of Humanities and Management Sciences, Southwest Medical University, Luzhou 646000, China. LA - eng GR - 2023YFC3304602/National Key Research and Development Program of China/ PT - Journal Article DEP - 20261019 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701340 OTO - NOTNLM OT - EEG OT - emotion recognition OT - knowledge distillation OT - knowledge transfer OT - single-channel EEG COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/19 CRDT- 2026/10/27 10:32 PHST- 2026/09/25 00:00 [received] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - bioengineering13101223 [pii] AID - bioengineering-13-01223 [pii] AID - 10.3390/bioengineering13101223 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 19;13(10):1223. doi: 10.3390/bioengineering13101223. PMID- 42939074 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 19 TI - Potential Mechanobiological Consequences of Injectable Aesthetic Procedures: Dermal Fillers, Botulinum Toxin, ECM Dynamics, Lymphovascular Drainage, and the Concept of Mechanical Silence. LID - 10.3390/bioengineering13101219 [doi] LID - 1219 AB - Injectable aesthetic procedures are commonly discussed in terms of volumization, muscle relaxation, wrinkle reduction, and adverse events, whereas their potential effects on the dynamic mechanical environment of facial soft tissues have received less attention. The dermis is a mechanosensitive, viscoelastic tissue in which fibroblasts, extracellular matrix (ECM), interstitial fluid, vascular and lymphatic physiology, and repeated facial motion interact. Established evidence demonstrates that appropriately used hyaluronic acid fillers can restore structural support and stimulate collagen remodeling, while botulinum toxin reduces muscle contraction and dynamic rhytides. By contrast, direct clinical evidence that injectable procedures produce sustained abnormalities in tissue mechanics, lymphatic pumping, or mechanotransductive signaling remains limited. This narrative review integrates established clinical evidence, mechanistic evidence from adjacent fields, and hypothesis-generating interpretations to propose the concept of injectable procedure-related "mechanical silence": a localized reduction or distortion of physiological tissue deformation, fluid exchange, and cell-matrix mechanical signaling. The concept is not presented as a demonstrated adverse effect or as evidence that fillers or botulinum toxin accelerate aging. Rather, it provides a testable framework for investigating whether selected patterns of excessive volume, altered local stiffness, persistent edema, or marked reduction in muscle-driven tissue motion are associated with measurable changes in tissue biomechanics. Pressure-injury biology is discussed only as a conceptual example of the consequences of sustained deformation and impaired tissue mobility and is not considered mechanically or clinically equivalent to aesthetic injectable treatment. Future studies should combine structural imaging with quantitative assessment of tissue stiffness, deformation and recoil, filler burden, lymphatic transit, perfusion, muscle excursion, and mechanotransductive biomarkers to determine whether the proposed framework has clinical relevance. FAU - Choi, Wonseok AU - Choi W AD - V Plastic Surgery, Daegu 41940, Republic of Korea. FAU - Yi, Kyu-Ho AU - Yi KH AUID- ORCID: 0000-0001-5572-1364 AD - Division in Anatomy and Developmental Biology, Department of Oral Biology, Human Identification Research Institute, BK21 FOUR Project, Yonsei University College of Dentistry, Seoul 03722, Republic of Korea. FAU - Sydorchuk, Olena AU - Sydorchuk O AUID- ORCID: 0009-0000-8491-7323 AD - Aesthefora Inc., Seoul 04364, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701878 OTO - NOTNLM OT - botulinum toxin OT - dermal filler OT - extracellular matrix OT - facial aging OT - fibroblast OT - lymphatic drainage OT - mechanical silence OT - mechanotransduction OT - pressure injury OT - vascular circulation COIS- Author Olena Sydorchuk was employed by the company Aesthefora Inc. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The company had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/19 CRDT- 2026/10/27 10:32 PHST- 2026/08/09 00:00 [received] PHST- 2026/09/23 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - bioengineering13101219 [pii] AID - bioengineering-13-01219 [pii] AID - 10.3390/bioengineering13101219 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 19;13(10):1219. doi: 10.3390/bioengineering13101219. PMID- 42939073 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 19 TI - Recent Advances in the Application of Nanocatalysts for the Hydrodeoxygenation of Lignin and Its Derivatives to Jet-Fuel-Range Cycloalkanes and Aromatics. LID - 10.3390/bioengineering13101218 [doi] LID - 1218 AB - Lignin is the sole renewable organic carbon resource that is intrinsically rich in aromatic rings. Its catalytic hydrodeoxygenation (HDO) to produce jet-fuel-range cycloalkanes and aromatics represents a pivotal route toward achieving sustainable aviation fuel. The central challenge of HDO lies in achieving selective C-O bond cleavage while precisely controlling the hydrogenation of aromatic rings. This review systematically summarizes recent advances in nanocatalysts for this application, covering molybdenum-based catalysts (edge-site engineering and oxygen vacancy regulation in MoS(2)), nickel-based catalysts (optimization of low-temperature activity in nickel phosphide and hydrogen spillover strategies), bimetallic catalysts (geometric/electronic/stabilization/synergistic effects), and bifunctional catalysts (synergy between acid sites and metal sites), with particular emphasis on the emerging single-atom catalysts (coordination environment and charge density regulation). The functional roles of nanocatalysts in cascade conversion processes are also discussed. Finally, the future directions for the rational design of highly efficient nanocatalytic systems are outlined, aiming to provide insights for the targeted synthesis of lignin-based jet-fuel-range cycloalkanes and aromatics. FAU - Gu, Kexin AU - Gu K AUID- ORCID: 0009-0002-2895-2049 AD - Department of Chemistry, Zhejiang University, Hangzhou 310058, China. FAU - Zhang, Dongyu AU - Zhang D AD - Department of Chemistry, Zhejiang University, Hangzhou 310058, China. FAU - Yin, Meiting AU - Yin M AD - Department of Chemistry, Zhejiang University, Hangzhou 310058, China. FAU - Yan, Shu AU - Yan S AD - Department of Chemistry, Zhejiang University, Hangzhou 310058, China. AD - Center of Chemistry for Frontier Technologies, Zhejiang University, Hangzhou 310058, China. FAU - Fang, Wenjun AU - Fang W AUID- ORCID: 0000-0002-5610-1623 AD - Department of Chemistry, Zhejiang University, Hangzhou 310058, China. AD - Center of Chemistry for Frontier Technologies, Zhejiang University, Hangzhou 310058, China. AD - Zhejiang Key Laboratory of Low-Carbon Synthesis of Value-Added Chemicals, Zhejiang University, Hangzhou 310058, China. FAU - Guo, Yongsheng AU - Guo Y AUID- ORCID: 0000-0001-7609-1891 AD - Department of Chemistry, Zhejiang University, Hangzhou 310058, China. AD - Center of Chemistry for Frontier Technologies, Zhejiang University, Hangzhou 310058, China. AD - Zhejiang Key Laboratory of Low-Carbon Synthesis of Value-Added Chemicals, Zhejiang University, Hangzhou 310058, China. LA - eng GR - 22573091/National Natural Science Foundation of China/ GR - 2025E10097/Zhejiang Key Laboratory of Low-Carbon Synthesis of Value-Added Chemicals/ PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701817 OTO - NOTNLM OT - hydrodeoxygenation OT - lignin OT - nanocatalysis OT - sustainable aviation fuel (SAF) COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/19 CRDT- 2026/10/27 10:32 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - bioengineering13101218 [pii] AID - bioengineering-13-01218 [pii] AID - 10.3390/bioengineering13101218 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 19;13(10):1218. doi: 10.3390/bioengineering13101218. PMID- 42939072 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 19 TI - Infrared Technology for Vein Visualization: Device Development. LID - 10.3390/bioengineering13101217 [doi] LID - 1217 AB - Difficult peripheral venous access can complicate routine venipuncture, especially when superficial veins are poorly visible under normal lighting conditions. This study aims to develop and preliminarily evaluate a low-cost near-infrared imaging prototype for non-invasive visualization of superficial veins. The prototype consists of a camera without an infrared light filter, an infrared LED illumination module, and an image-processing pipeline implemented in MATLAB. Acquired images were processed using median filtering, histogram-based contrast enhancement, binarization, inversion, and masking procedures to improve vein visibility and outline vascular structures. The prototype was tested on three participants, with three anatomical regions examined for each participant, resulting in nine analyzed images. Vein-to-background contrast and contrast-to-noise ratio were calculated before and after image processing. Across the analyzed images, mean contrast increased from 0.14968 to 0.29088, with a mean relative improvement of 113.40%, while mean CNR increased from 0.48179 to 1.00729, with a mean relative improvement of 130.65%. These results indicate that the proposed system improved the objective visibility of superficial veins under controlled acquisition conditions. The study provides an initial technical basis for further development, while further studies are required to evaluate its performance in larger and more diverse participant groups. FAU - Ivanov, Nicholas V AU - Ivanov NV AUID- ORCID: 0009-0007-1772-6018 AD - Department of Electrical Engineering and Measurement, Faculty of Electrical Engineering, Technical University of Cluj-Napoca, 400114 Cluj-Napoca, Romania. FAU - Nicu, Anca-Iulia AU - Nicu AI AUID- ORCID: 0000-0001-9429-9530 AD - Department of Electrical Engineering and Measurement, Faculty of Electrical Engineering, Technical University of Cluj-Napoca, 400114 Cluj-Napoca, Romania. FAU - Roman, Nicolae Marius AU - Roman NM AUID- ORCID: 0009-0002-9846-4884 AD - Department of Electrical Engineering and Measurement, Faculty of Electrical Engineering, Technical University of Cluj-Napoca, 400114 Cluj-Napoca, Romania. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701687 OTO - NOTNLM OT - NIR OT - filter OT - image processing OT - segmentation OT - veins COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/19 CRDT- 2026/10/27 10:32 PHST- 2026/07/16 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - bioengineering13101217 [pii] AID - bioengineering-13-01217 [pii] AID - 10.3390/bioengineering13101217 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 19;13(10):1217. doi: 10.3390/bioengineering13101217. PMID- 42939069 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 19 TI - Advancements in Clinical Applications of Transcranial Magnetic Stimulation Using Different Coil Configurations: A Review. LID - 10.3390/bioengineering13101214 [doi] LID - 1214 AB - The effects of transcranial magnetic stimulation (TMS) are jointly determined by coil geometry, intracranial electric-field distribution, stimulation target, and pulse parameters, with coil design directly governing stimulation depth, spatial focality, and brain tissue coverage. This review compares circular, figure-of-eight, double-cone, and H-coils in terms of geometry, electric-field distribution, effective depth, focality, and current clinical use. Figure-of-eight coils provide high superficial focality and are suited to anatomically defined cortical targets such as the dorsolateral prefrontal cortex, primary motor cortex, and supplementary motor area. Double-cone and H-coils extend effective electric-field coverage to medial and relatively deep networks but reduce spatial selectivity, illustrating the depth-focality trade-off. The maturity of clinical evidence varies across disorders. Conventional repetitive TMS, intermittent theta-burst stimulation, and H1-coil deep TMS have comparatively established evidence in depression, whereas stroke and migraine remain heterogeneous in terms of target selection, dose, and patient selection. Key challenges are to match individualized intracranial electric-field dose, disease-network targets, pulse sequences, and brain state. This work offers valuable guidance for researchers in related fields and professionals engaged in the clinical management of neuropsychiatric disorders. FAU - Zhang, Wentao AU - Zhang W AD - Key Laboratory of Opto-Electronic Technology and Intelligent Control of Ministry of Education, Lanzhou Jiaotong University, Lanzhou 730070, China. FAU - Lu, Mai AU - Lu M AUID- ORCID: 0000-0001-7192-9771 AD - Key Laboratory of Opto-Electronic Technology and Intelligent Control of Ministry of Education, Lanzhou Jiaotong University, Lanzhou 730070, China. LA - eng GR - 52467026/National Natural Science Foundation of China/ GR - 2025-XZ-21/Chinese Academy of Engineering/ GR - 2024CXPT-11/Gansu Education Department/ PT - Journal Article PT - Review DEP - 20261019 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701443 OTO - NOTNLM OT - TMS OT - coil configuration OT - neurological disorder OT - neuromodulation OT - psychiatric disorder COIS- The authors declare no conflicts of interest. The funders had no role in the design of this study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/19 CRDT- 2026/10/27 10:32 PHST- 2026/08/18 00:00 [received] PHST- 2026/10/01 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - bioengineering13101214 [pii] AID - bioengineering-13-01214 [pii] AID - 10.3390/bioengineering13101214 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 19;13(10):1214. doi: 10.3390/bioengineering13101214. PMID- 42939063 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 16 TI - Physiology-Informed Blink Artefact Detection from Bilateral Frontal EEG Under Asymmetric Interference. LID - 10.3390/bioengineering13101208 [doi] LID - 1208 AB - Eye blinks contaminate frontal electroencephalography (EEG), while asymmetric disturbances can cause false detections. We present a physiology-informed detector that combines waveform correlation, peak-time agreement, amplitude similarity, and symmetric-component dominance in an explicit event-verification rule. It uses two frontal channels without supervised model fitting. Evaluation on raw ZuCo 1.0 recordings included 260 eligible blocks from 12 participants, totalling 1291.61 min and 8611 eye-tracker reference intervals. Scalp and periocular pairs were evaluated separately. On the scalp pair, participant-mean precision was 0.731, recall 0.774, F1 0.734, and false positives 1.469 per minute. At fixed settings, false-positive rates were lower than with single-channel thresholding and bilateral averaging (both Holm-adjusted p = 0.005859), and remained lower than those of four rule-based controls across five perturbation conditions. Threshold-selected simple controls achieved higher precision at similar recall. Component ablations quantified the effects of the verification checks on sensitivity and false positives. The offline Python detector required a median of 2.13 ms per minute of recorded data on an Apple M1 Pro. These results establish a computationally inexpensive approach that makes blink-event decisions directly inspectable through bilateral signal measurements. FAU - Zhang, Weiqiang AU - Zhang W AD - College of Biomedical Engineering, Fudan University, Shanghai 200433, China. AD - Human Phenome Institute, Fudan University, Shanghai 201203, China. FAU - Li, Menglong AU - Li M AD - Human Phenome Institute, Fudan University, Shanghai 201203, China. FAU - Zhang, Yujie AU - Zhang Y AD - Human Phenome Institute, Fudan University, Shanghai 201203, China. FAU - Cao, Jingyi AU - Cao J AUID- ORCID: 0000-0002-1420-7489 AD - Department of Cardiovascular Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China. FAU - Shen, Feng AU - Shen F AD - Department of Cardiovascular Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China. FAU - Liu, Xiaohui AU - Liu X AD - Micro-Nano System Centre, School of Information Science and Technology, Fudan University, Shanghai 200433, China. FAU - Chen, Chen AU - Chen C AD - Human Phenome Institute, Fudan University, Shanghai 201203, China. AD - Center for Medical Research and Innovation, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai 201399, China. FAU - Li, Yang AU - Li Y AD - Human Phenome Institute, Fudan University, Shanghai 201203, China. AD - Department of Cardiovascular Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China. LA - eng GR - 82470383/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701908 OTO - NOTNLM OT - bilateral verification OT - blink detection OT - electroencephalography OT - ocular artefacts OT - signal processing COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/16 CRDT- 2026/10/27 10:32 PHST- 2026/08/27 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - bioengineering13101208 [pii] AID - bioengineering-13-01208 [pii] AID - 10.3390/bioengineering13101208 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 16;13(10):1208. doi: 10.3390/bioengineering13101208. PMID- 42939061 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 16 TI - Evaluation of Wearable Sensor Placement for Movement Bout Duration Estimation: A Comparative Feasibility Study. LID - 10.3390/bioengineering13101206 [doi] LID - 1206 AB - Wearable sensor placement can influence movement quantification, yet direct comparisons across anatomical sites remain limited for bout-level temporal outcomes. This study evaluated seven IMU placements for movement bout duration estimation. Twenty-seven healthy adults performed three whole-body tasks (walking, Timed Up and Go, and Step Test) and two upper-limb tasks (reaching and hand-to-mouth) under concurrent optoelectronic motion capture. IMU-derived durations were compared with segment-specific local references and, for whole-body tasks, a common sacral reference used as a proxy for whole-body movement. Agreement was assessed using Spearman's correlation, ICC, Bland-Altman analysis, and MAPE. Participant-level agreement analyses were complemented by trial-level repeated-measures analyses to account for within-participant variability across repeated trials. A descriptive composite ranking combined ICC, MAPE, and limits-of-agreement width against the common reference. Performance varied across placements and tasks, with the largest local-common dissociation observed during the TUG. The sacrum obtained the most favorable overall composite ranking score (2.78), closely followed by the dominant ankle (2.89) and non-dominant wrist (3.11). Both wrists showed high agreement during upper-limb tasks (ICC = 0.879-0.994). Trial-level analyses confirmed a significant effect of sensor placement for all whole-body tasks, while no significant differences were observed between wrist placements during the isolated upper-limb tasks. The non-dominant wrist showed a favorable combination of performance across movement domains, supporting its selection as a promising candidate for further evaluation in subsequent free-living monitoring. FAU - Cerfoglio, Serena AU - Cerfoglio S AUID- ORCID: 0000-0002-1983-8204 AD - Department of Electronics, Information and Bioengineering, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy. AD - IRCCS Istituto Auxologico Italiano, San Giuseppe Hospital, Strada Luigi Cadorna 90, 28824 Piancavallo, Italy. FAU - Donno, Lucia AU - Donno L AUID- ORCID: 0000-0003-4297-2433 AD - Department of Electronics, Information and Bioengineering, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy. AD - E4Sport-Human Peformance Lab, Polo Territoriale di Lecco, Politecnico di Milano, Via G. Previati 1/c, 23900 Lecco, Italy. FAU - Lovato, Mattia AU - Lovato M AUID- ORCID: 0009-0004-9248-5603 AD - Department of Electronics, Information and Bioengineering, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy. AD - E4Sport-Human Peformance Lab, Polo Territoriale di Lecco, Politecnico di Milano, Via G. Previati 1/c, 23900 Lecco, Italy. FAU - Francia, Carlalberto AU - Francia C AUID- ORCID: 0009-0009-9454-7181 AD - Department of Electronics, Information and Bioengineering, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy. AD - E4Sport-Human Peformance Lab, Polo Territoriale di Lecco, Politecnico di Milano, Via G. Previati 1/c, 23900 Lecco, Italy. FAU - Galli, Manuela AU - Galli M AUID- ORCID: 0000-0003-2772-4837 AD - Department of Electronics, Information and Bioengineering, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy. AD - E4Sport-Human Peformance Lab, Polo Territoriale di Lecco, Politecnico di Milano, Via G. Previati 1/c, 23900 Lecco, Italy. FAU - Cimolin, Veronica AU - Cimolin V AUID- ORCID: 0000-0001-6299-7254 AD - Department of Electronics, Information and Bioengineering, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy. AD - IRCCS Istituto Auxologico Italiano, San Giuseppe Hospital, Strada Luigi Cadorna 90, 28824 Piancavallo, Italy. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701408 OTO - NOTNLM OT - free-living monitoring OT - inertial measurement units OT - movement segmentation OT - sensor placement OT - temporal accuracy OT - wearable sensors COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/16 CRDT- 2026/10/27 10:32 PHST- 2026/09/24 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - bioengineering13101206 [pii] AID - bioengineering-13-01206 [pii] AID - 10.3390/bioengineering13101206 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 16;13(10):1206. doi: 10.3390/bioengineering13101206. PMID- 42939060 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 16 TI - Triaxial Accelerometry Sensor for Respiratory-Effort Phase Landmark Detection During Sleep: A Cross-Modal Weak Supervision Approach. LID - 10.3390/bioengineering13101205 [doi] LID - 1205 AB - Reliable respiratory phase sensing is important for respiratory-synchronized hypoglossal nerve stimulation (HGNS), but conventional sensing approaches may impose additional burden. This pilot study investigated whether a surface triaxial accelerometry sensor (TAS) could detect thoracic respiratory inductance plethysmography (RIP) troughs, which were used as operational phase landmarks representing the transition from end expiration to subsequent inspiration, rather than as directly established physiological respiratory onsets. Ten participants underwent synchronized overnight polysomnography and surface TAS recording, providing 5377.3 min of analyzable data and 75,499 automatically generated RIP landmarks. Two complementary Conformer models were trained using Gaussian weak labels and combined using multi-offset dual-conformer fusion. Generalization was evaluated using outer leave-one-subject-out validation, with fusion weights and event thresholds selected from training-side validation data. At a +/-1.0 s matching tolerance, the fusion achieved participant-mean precision, recall, and F1 scores of 0.78, 0.82, and 0.80, respectively, with a 95% confidence interval of 0.75-0.85 for the mean F1 score. It outperformed each component model in 9 of 10 participants; however, its F1 gain over otherwise identical single-grid fusion was 0.0036, below the prespecified exploratory advancement margin. These findings provide preliminary evidence that surface TASs can recover an RIP-trough-derived operational phase landmark in unseen same-center participants. Independent multimodal adjudication, external replication, and full-night causal testing are required before clinical or respiratory-synchronized HGNS applications. FAU - Lv, Mengshuang AU - Lv M AD - Obstructive Sleep Apnea-Hypopnea Syndrome Clinical Diagnosis and Therapy and Research Centre, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, China. AD - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Capital Medical University, Ministry of Education, Beijing 100005, China. FAU - Liao, Jianhong AU - Liao J AUID- ORCID: 0000-0003-2465-3850 AD - Obstructive Sleep Apnea-Hypopnea Syndrome Clinical Diagnosis and Therapy and Research Centre, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, China. AD - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Capital Medical University, Ministry of Education, Beijing 100005, China. FAU - Shi, Yunhan AU - Shi Y AD - Obstructive Sleep Apnea-Hypopnea Syndrome Clinical Diagnosis and Therapy and Research Centre, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, China. AD - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Capital Medical University, Ministry of Education, Beijing 100005, China. FAU - Zhang, Bowen AU - Zhang B AD - Obstructive Sleep Apnea-Hypopnea Syndrome Clinical Diagnosis and Therapy and Research Centre, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, China. AD - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Capital Medical University, Ministry of Education, Beijing 100005, China. FAU - Zeng, Qiying AU - Zeng Q AD - Obstructive Sleep Apnea-Hypopnea Syndrome Clinical Diagnosis and Therapy and Research Centre, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, China. AD - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Capital Medical University, Ministry of Education, Beijing 100005, China. FAU - Li, Yanru AU - Li Y AD - Obstructive Sleep Apnea-Hypopnea Syndrome Clinical Diagnosis and Therapy and Research Centre, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, China. AD - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Capital Medical University, Ministry of Education, Beijing 100005, China. AD - Key Laboratory of Biopharmaceuticals and Medical Devices, Xinjiang Medical University, Urumqi 830054, China. FAU - Han, Demin AU - Han D AD - Obstructive Sleep Apnea-Hypopnea Syndrome Clinical Diagnosis and Therapy and Research Centre, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, China. AD - Key Laboratory of Otorhinolaryngology Head and Neck Surgery, Capital Medical University, Ministry of Education, Beijing 100005, China. AD - Key Laboratory of Biopharmaceuticals and Medical Devices, Xinjiang Medical University, Urumqi 830054, China. LA - eng GR - 2024-XZ- 64/Chinese Academy of Engineering/ GR - XZ202501ZY0060/Tibet Autonomous Region Science and Technology Agency/ GR - L243032/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701435 OTO - NOTNLM OT - respiratory phase-landmark detection OT - triaxial accelerometry sensor OT - weak supervision COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/16 CRDT- 2026/10/27 10:32 PHST- 2026/09/11 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/03 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - bioengineering13101205 [pii] AID - bioengineering-13-01205 [pii] AID - 10.3390/bioengineering13101205 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 16;13(10):1205. doi: 10.3390/bioengineering13101205. PMID- 42939059 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 16 TI - Artificial Intelligence in Bioengineering: Innovations, Challenges, and Future Directions: A Narrative Review. LID - 10.3390/bioengineering13101204 [doi] LID - 1204 AB - Artificial intelligence (AI) is rapidly transforming bioengineering, with applications ranging from diagnosis and biomedical imaging to wearable technologies, rehabilitation, biomaterials, biofabrication, and digital twins. This narrative review aims to examine the major innovations, challenges, and future directions of AI-enabled bioengineering, with particular attention to its clinical and translational development. The literature was searched in Scopus and PubMed, with emphasis on systematic reviews and meta-analyses and consideration of other relevant secondary literature across the main bioengineering domains. A total of 57 secondary publications were included in the principal evidence base. The review process was additionally informed by the ANDJ Narrative Review Checklist and an established qualification and scoring methodology applied to the selected secondary literature. The reviewed evidence shows a broad expansion of AI applications and increasing integration of intelligent technologies into bioengineering systems. However, important challenges remain, particularly regarding data quality and representativeness, validation and generalizability, reproducibility, safety, human oversight, ethical responsibility, equity, and regulatory requirements. Future developments are expected to increasingly involve multimodal, adaptive, connected, and human-centered systems integrating AI with sensing technologies, robotics, digital twins, and longitudinal data. Overall, AI-enabled bioengineering is progressing from promising technological applications toward increasingly integrated and translational systems. Its successful evolution will depend on combining technological innovation with robust clinical evidence, responsible development, and appropriate ethical and regulatory frameworks. FAU - Giansanti, Daniele AU - Giansanti D AD - Centro IATIS, Istituto Superiore di Sanita, 00161 Roma, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701786 OTO - NOTNLM OT - artificial intelligence OT - bioengineering OT - biomedical engineering OT - digital health OT - digital twins OT - intelligent systems OT - machine learning OT - medical imaging OT - robotics OT - wearable technologies COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/16 CRDT- 2026/10/27 10:32 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - bioengineering13101204 [pii] AID - bioengineering-13-01204 [pii] AID - 10.3390/bioengineering13101204 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 16;13(10):1204. doi: 10.3390/bioengineering13101204. PMID- 42939058 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 16 TI - Label-Free Biochemical Characterization and Subtype Classification of Human Breast Pathologies Using Fingerprint Raman Spectroscopy and Machine Learning. LID - 10.3390/bioengineering13101203 [doi] LID - 1203 AB - Breast cancer remains a major cause of cancer-related mortality, highlighting the need for improved molecular characterization of breast pathologies. Raman spectroscopy (RS) provides a label-free, non-destructive approach for probing tissue biochemical composition. This study investigated Raman spectral alterations across five human breast histopathological categories and explored their potential for machine learning-assisted classification. Formalin-fixed, paraffin-embedded (FFPE) tissue specimens were obtained from five patients, with one patient representing each category: normal breast tissue, intraductal papilloma, high-grade ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC), and invasive lobular carcinoma (ILC). A total of 100 Raman spectra (20 per patient) were acquired using 785 nm excitation over the fingerprint region (500-2000 cm(-1)). Spectral preprocessing involved Savitzky-Golay smoothing, asymmetrically reweighted penalized least-squares (arPLS) baseline correction, and vector normalization. The investigated spectra exhibited reduced relative lipid-associated contributions near 1063, 1300, and 1445 cm(-1), accompanied by increased relative protein- and nucleic-acid-associated features near 1003, 1420, and 1600-1650 cm(-1). The phenylalanine-to-lipid intensity ratio (I(1003)/I(1445)) increased from /=1.5 in invasive lesions, suggesting a potential spectroscopic descriptor of biochemical remodeling. Exploratory subtype-associated differences were also observed, including a prominent aromatic C=C-associated feature near 1618 cm(-1) in IDC and an enhanced carbohydrate-associated contribution near 890 cm(-1) in ILC. A support vector machine (SVM) classifier was evaluated using patient-independent leave-one-patient-out (LOPO) cross-validation. Across the five held-out patients, the model correctly classified 89 of 100 spectra, achieving an overall spectrum-level accuracy of 89.0%, sensitivity of 90.0%, specificity of 87.5%, and F1-score of 90.8%. Although these findings demonstrate encouraging discrimination within the investigated cohort, the limited number of biologically independent patients and the representation of each histopathological category by a single patient restrict the generalizability of the results. This study provides preliminary proof-of-concept evidence supporting the integration of Raman-derived biochemical features with machine learning for breast pathology characterization, warranting further validation in larger, multi-patient and multicenter cohorts. FAU - Van Hung, Nguyen AU - Van Hung N AD - Department of Radiation Physics, K Hospital (Tan Trieu Campus), Hanoi 100000, Vietnam. AD - Faculty of Engineering Physics, Hanoi University of Science and Technology, No. 1 Dai Co Viet, Hanoi 100000, Vietnam. FAU - Thuan, Mai Huu AU - Thuan MH AD - Faculty of Engineering Physics, Hanoi University of Science and Technology, No. 1 Dai Co Viet, Hanoi 100000, Vietnam. FAU - Quan, Ngo Duc AU - Quan ND AUID- ORCID: 0000-0001-5654-7134 AD - Faculty of Engineering Physics, Hanoi University of Science and Technology, No. 1 Dai Co Viet, Hanoi 100000, Vietnam. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701730 OTO - NOTNLM OT - Raman spectroscopy OT - biochemical characterization OT - breast pathology OT - leave-one-patient-out validation OT - machine learning OT - support vector machine COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/16 CRDT- 2026/10/27 10:32 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - bioengineering13101203 [pii] AID - bioengineering-13-01203 [pii] AID - 10.3390/bioengineering13101203 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 16;13(10):1203. doi: 10.3390/bioengineering13101203. PMID- 42939056 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 15 TI - Research and Application of Tongue Image Segmentation Method Based on Lightweight Deep Learning. LID - 10.3390/bioengineering13101201 [doi] LID - 1201 AB - Intelligent tongue diagnosis demands high-precision segmentation on resource-constrained mobile devices. All deep learning models outperform conventional approaches, improving mean intersection over union (mIoU) by approximately 9% and accelerating inference by more than two orders of magnitude. Among the evaluated models, UNet++ achieves the highest test mIoU (0.9543) and Dice (0.9764), with paired bootstrap analysis confirming statistically significant advantages over BiSeNet, PP-LiteSeg, and HRNet. BiSeNet achieves the fastest inference speed (310.03 FPS) and the highest mPA (0.9766), making it the most efficient model for real-time deployment. HRNet and PP-LiteSeg provide a favorable accuracy-efficiency balance, suitable for mobile-based tongue diagnosis. Deployment of PP-LiteSeg on three Android devices achieved inference latency of 233-262 ms and throughput of 3.2-3.4 FPS, demonstrating the feasibility of lightweight models on commodity mobile hardware. These findings establish an evidence-based framework for model selection in mobile tongue diagnosis, supporting AI deployment on portable devices for clinical assistance and remote monitoring. FAU - Gou, Yulin AU - Gou Y AD - School of Intelligent Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. FAU - Zhang, Ziyang AU - Zhang Z AD - School of Intelligent Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. FAU - Song, Haibei AU - Song H AD - School of Intelligent Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. FAU - Wang, Alan AU - Wang A AUID- ORCID: 0000-0003-1610-2397 AD - Auckland Bioengineering Institute, The University of Auckland, Auckland 1142, New Zealand. AD - Medical Imaging Research Center, Faculty of Medical and Health Sciences, The University of Auckland, Auckland 1023, New Zealand. AD - Centre for Co-Created Ageing Research, The University of Auckland, Auckland 1023, New Zealand. AD - Centre for Brain Research, The University of Auckland, Auckland 1023, New Zealand. LA - eng GR - 82004504/National Natural Science Foundation of China/ GR - 2021MS199/Sichuan Provincial Administration of Traditional Chinese Medicine/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701977 OTO - NOTNLM OT - lightweight deep learning models OT - mobile device deployment OT - objective representation of traditional Chinese medicine OT - semantic segmentation OT - tongue diagnosis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/15 CRDT- 2026/10/27 10:32 PHST- 2026/08/24 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - bioengineering13101201 [pii] AID - bioengineering-13-01201 [pii] AID - 10.3390/bioengineering13101201 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 15;13(10):1201. doi: 10.3390/bioengineering13101201. PMID- 42939052 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 15 TI - Effects of Group Postural Rehabilitation in Patients with Parkinson's Disease and Camptocormia: Preliminary Data from an Observational Pilot Study. LID - 10.3390/bioengineering13101197 [doi] LID - 1197 AB - Background and Objectives: Parkinson's disease (PD) is frequently associated with postural instability, including camptocormia, an involuntary anterior flexion of the thoracolumbar spine. This retrospective observational pilot study evaluated the effects of a postural group rehabilitation protocol on the stabilometric parameters, depressive symptoms, and quality of life in PD patients with camptocormia, and whether gains were maintained during a subsequent two-month period after completion of supervised group rehabilitation, during which patients were provided with a general home-based exercise program. Materials and Methods: Forty patients with idiopathic PD (Hoehn and Yahr II-III) were assessed at baseline (T0), after two months of twice-weekly rehabilitation (T1). At T2, only stabilometric follow-up was performed after two months without group exercise for 38 patients. Outcomes included SPPB, Tinetti, Berg Balance Scale, Hamilton Depression Rating Scale, PDQ-39, occiput-to-wall distance and stabilometric analysis. Results: At T1, Hamilton scores decreased from 11.44 +/- 4.59 to 3.33 +/- 3.04 and PDQ-39 from 82.22 +/- 22.36 to 69.72 +/- 16.54 (both p < 0.001). Occiput-to-wall distance improved significantly in three of six positions. The EO/EC Perimeter Ratio decreased significantly from T0 to T1 (p = 0.016). Between T1 and T2, EO Perimeter continued improving (p = 0.010), whereas the Perimeter Ratio worsened (p = 0.046), and EC Ellipse area, though non-significant, increased rather than decreased. Conclusions: A two-month protocol was followed by apparent improvements in depression, quality of life, postural alignment, and selected stabilometric parameters. After discontinuation, some parameters appeared to improve while others seemed to worsen, suggesting these effects may not be sustained with uniformity. These preliminary findings should be confirmed with an expanded sample size in larger studies. FAU - Calciano, Rossella AU - Calciano R AUID- ORCID: 0009-0006-9069-5089 AD - Department of Geriatrics, Orthopedics and Rheumatology, Fondazione Policlinico Universitario "Agostino Gemelli" IRCCS, L.go A. Gemelli 8, 00168 Rome, Italy. FAU - Ferrara, Paola Emilia AU - Ferrara PE AD - Department of Geriatrics, Orthopedics and Rheumatology, Fondazione Policlinico Universitario "Agostino Gemelli" IRCCS, L.go A. Gemelli 8, 00168 Rome, Italy. FAU - Cutaia, Alberto AU - Cutaia A AUID- ORCID: 0009-0008-7427-238X AD - University Department of Geriatric and Orthopedic Sciences, Universita Cattolica del Sacro Cuore, L.go F. Vito 1, 00168 Rome, Italy. FAU - Tarallo, Letizia AU - Tarallo L AD - University Department of Geriatric and Orthopedic Sciences, Universita Cattolica del Sacro Cuore, L.go F. Vito 1, 00168 Rome, Italy. FAU - Capolongo, Virginia AU - Capolongo V AD - University Department of Geriatric and Orthopedic Sciences, Universita Cattolica del Sacro Cuore, L.go F. Vito 1, 00168 Rome, Italy. FAU - Cioffi, Rosa AU - Cioffi R AD - University Department of Geriatric and Orthopedic Sciences, Universita Cattolica del Sacro Cuore, L.go F. Vito 1, 00168 Rome, Italy. FAU - Ricciardi, Diego AU - Ricciardi D AD - Department of Geriatrics, Orthopedics and Rheumatology, Fondazione Policlinico Universitario "Agostino Gemelli" IRCCS, L.go A. Gemelli 8, 00168 Rome, Italy. AD - University Department of Geriatric and Orthopedic Sciences, Universita Cattolica del Sacro Cuore, L.go F. Vito 1, 00168 Rome, Italy. FAU - Tassi, Ludovica AU - Tassi L AUID- ORCID: 0009-0000-4287-1849 AD - Department of Geriatrics, Orthopedics and Rheumatology, Fondazione Policlinico Universitario "Agostino Gemelli" IRCCS, L.go A. Gemelli 8, 00168 Rome, Italy. FAU - Lo Monaco, Maria Rita AU - Lo Monaco MR AD - Department of Geriatrics, Orthopedics and Rheumatology, Fondazione Policlinico Universitario "Agostino Gemelli" IRCCS, L.go A. Gemelli 8, 00168 Rome, Italy. AD - Geriatrics Unit, Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Rome, Italy. FAU - Coraci, Daniele AU - Coraci D AUID- ORCID: 0000-0002-7019-9006 AD - Section of Rehabilitation, Department of Neuroscience-DNS, University of Padova, 35122 Padova, Italy. FAU - Ronconi, Gianpaolo AU - Ronconi G AD - Department of Geriatrics, Orthopedics and Rheumatology, Fondazione Policlinico Universitario "Agostino Gemelli" IRCCS, L.go A. Gemelli 8, 00168 Rome, Italy. AD - University Department of Geriatric and Orthopedic Sciences, Universita Cattolica del Sacro Cuore, L.go F. Vito 1, 00168 Rome, Italy. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701349 OTO - NOTNLM OT - Parkinson's disease OT - camptocormia OT - falls OT - postural control OT - postural exercise OT - quality of life OT - stabilometric analysis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/15 CRDT- 2026/10/27 10:32 PHST- 2026/09/04 00:00 [received] PHST- 2026/10/04 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - bioengineering13101197 [pii] AID - bioengineering-13-01197 [pii] AID - 10.3390/bioengineering13101197 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 15;13(10):1197. doi: 10.3390/bioengineering13101197. PMID- 42939048 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 14 TI - Exploring the Acute Effects of Stimulating Different Muscle Combinations During Neuromuscular Electrical Stimulation-Assisted Treadmill Walking in Children with Cerebral Palsy. LID - 10.3390/bioengineering13101193 [doi] LID - 1193 AB - Progressive gait impairments remain a major challenge for children with cerebral palsy (CP), often limiting independence and quality of life. Although neuromuscular electrical stimulation (NMES) has shown promise for improving gait, most NMES-assisted gait studies in children with CP have targeted only a single muscle. In this exploratory study, we characterized acute biomechanical responses of six children with CP to seven NMES protocols during treadmill walking. The protocols comprised multi-muscle stimulation, single-muscle stimulation, and an individualized physical therapist-derived (PT-derived) condition. Each protocol was compared with a no-stimulation baseline and typically developing (TD) reference data. The primary and secondary outcomes were the Gait Deviation Index (GDI) and Coefficient of Variation (CV), complemented by the targeted outcome measures, including lower-limb joint kinematics using phase-by-phase Dynamic Time Warping (DTW), maximum knee extension, and stride length. We found that the comparison between multi-muscle and single-muscle conditions did not identify a statistically significant difference in GDI. CV analyses showed smaller distances from TD reference values for multi-muscle conditions, although not significant. Stimulation conditions significantly affected maximum knee extension and DTW during loading response at the ankle and pre-swing at the hip; however, no individual condition differed significantly from no stimulation after false discovery rate correction. The PT-derived condition showed favorable directional changes in selected outcomes, but no consistent advantage over the other protocols. These preliminary observations characterize acute response patterns and generate hypotheses for future studies; they do not establish the comparative efficacy of multi-muscle, single-muscle, or individualized NMES protocols. FAU - Masaei, Mobina AU - Masaei M AUID- ORCID: 0009-0007-0408-9822 AD - Department of Biomechanics, University of Nebraska Omaha, Omaha, NE 68182, USA. FAU - Zahradka, Nicole AU - Zahradka N AD - Pediatric Mobility Laboratory, Department of Physical Therapy, University of Delaware, Newark, DE 19716, USA. FAU - Verma, Khushboo AU - Verma K AUID- ORCID: 0000-0002-3127-2063 AD - Pediatric Mobility Laboratory, Department of Physical Therapy, University of Delaware, Newark, DE 19716, USA. FAU - Lee, Samuel C K AU - Lee SCK AUID- ORCID: 0000-0002-3749-2665 AD - Pediatric Mobility Laboratory, Department of Physical Therapy, University of Delaware, Newark, DE 19716, USA. FAU - Behboodi, Ahad AU - Behboodi A AUID- ORCID: 0000-0003-4193-4769 AD - Department of Biomechanics, University of Nebraska Omaha, Omaha, NE 68182, USA. LA - eng GR - 5P20GM109090-06P06/NH/NIH HHS/United States GR - P20 GM109090/GM/NIGMS NIH HHS/United States GR - P30 GM103333/GM/NIGMS NIH HHS/United States GR - 71011-PHI,/Shriners Hospitals for Children - Philadelphia/ GR - 5P30GM103333-01P01/NH/NIH HHS/United States PT - Journal Article DEP - 20261014 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701523 OTO - NOTNLM OT - CP OT - Coefficient of Variation (CV) OT - Dynamic Time Warping (DTW) OT - Gait Deviation Index (GDI) OT - NMES OT - gait analysis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/14 CRDT- 2026/10/27 10:32 PHST- 2026/09/03 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - bioengineering13101193 [pii] AID - bioengineering-13-01193 [pii] AID - 10.3390/bioengineering13101193 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 14;13(10):1193. doi: 10.3390/bioengineering13101193. PMID- 42939039 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 12 TI - Uncertainty-Aware Selective Prediction in Brain-Tumour MRI Classification: Selective Risk Reduction and Component-Wise Reliability Findings. LID - 10.3390/bioengineering13101184 [doi] LID - 1184 AB - BACKGROUND: Automated brain-MRI analysis is usually judged by accuracy at a single operating point, yet a model that is accurate on average may still fail without signalling that it is about to fail. Selective prediction addresses this by allowing a model to abstain, but the resulting trade-off between coverage and risk is rarely quantified in medical imaging. OBJECTIVE: This study asks whether confidence- and entropy-based selective prediction produces a measurable reduction in selective risk in a biomedical vision-language classifier by concentrating errors among withheld cases, and at what coverage cost. Three further components of a brain-MRI-analysis framework-volumetric segmentation, glioma grading and structured report generation-are evaluated as complementary evidence about the reliability profiles of different modules, not as independent contributions. METHODS: A LoRA-fine-tuned LLaVA-Med classifier was evaluated on the 1000-image BRISC2025 test partition, with a disjunctive rejection rule based on softmax confidence and predictive entropy. Volumetric segmentation used nnU-Net v2 (3D full-resolution) on BraTS2020 with a three-fold ensemble evaluated on 74 held-out cases; glioma grading (high- versus low-grade) used DenseNet121 on multi-slice representations; report generation used Meta Llama 3.2 3B Instruct fine-tuned with QLoRA on structured findings. All proportions are reported with 95% Wilson score intervals under a single analysis plan documented before the reported analyses were finalised. RESULTS: Withholding 1.80% of predictions (95% CI 1.14-2.83) raised selective accuracy to 97.76% (95% CI 96.63-98.52) and reduced selective risk from 3.40% to 2.24%, a relative reduction of 34.1%. Withheld predictions were strongly enriched for errors (66.7% versus 2.24%; odds ratio 87.3, Fisher exact p = 4.6 x 10(-15)), capturing 35.3% of all errors while issuing 99.38% of the predictions that were correct. Considered as a detector of its own errors, the rule has a balanced accuracy of 67.34%; this describes the binary rejection decision at the realised threshold and does not identify the AUROC of the underlying continuous score, which the single operating point leaves anywhere in [0.351, 0.996]. Because the per-sample probability vectors were not retained, the full risk-coverage curve, the rule ablations and calibration error could not be computed, and the thresholds (tau(c) = 0.6, tau(H) = 1.0) were selected empirically on an undocumented partition; the selective-prediction finding is therefore reported as a single-operating-point case study and is exploratory. Among the complementary components, three-fold ensembling improved held-out mean Dice from 0.7948 to 0.8150 (+0.0202), reported descriptively because the per-case values needed for a paired test were not preserved. Glioma grading did not achieve better-than-chance discrimination: balanced accuracy 49.91%, Cohen's kappa -0.0023, Matthews correlation coefficient -0.0026, low-grade recall 8.96% (95% CI 5.81-13.57), and accuracy (74.03%) below a constant majority-class classifier (79.46%); the associated McNemar statistic is reported descriptively because slice-level observations are not independent. Report generation produced high automatic similarity scores (BERTScore F1 0.9513) that are not interpretable without baselines. CONCLUSIONS: Confidence- and entropy-based abstention produced a measurable reduction in selective risk at low rejection rates, and it did so because errors concentrated among the cases were withheld by the confidence/entropy rule rather than because predictions were discarded indiscriminately. At the realised coverage the rule captured 65.5% (95% bootstrap CI 41.9-87.8) of the reduction achievable by an oracle that rejects only true errors. The component-wise comparison shows that this reliability behaviour cannot be assumed for every module: high aggregate accuracy and useful discrimination came apart in the grading task. These findings are component-wise and do not constitute an end-to-end, externally validated or clinically validated pipeline. The vision-language evaluation is at image level: BRISC2025 carries no patient identifiers, so patient-level independence cannot be established and the reported intervals should be read with that constraint in mind. FAU - Alshamlan, Hala AU - Alshamlan H AUID- ORCID: 0000-0002-8427-5292 AD - Department of Information Technology, College of Computer and Information Sciences, King Saud University, P.O. Box 51178, Riyadh 11543, Saudi Arabia. LA - eng GR - ORF-2026-904/King Saud University/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701398 OTO - NOTNLM OT - 3D segmentation OT - BraTS2020 OT - brain-tumour MRI OT - error detection OT - glioma OT - nnU-Net OT - radiology report generation OT - risk-coverage analysis OT - selective classification OT - selective prediction OT - uncertainty quantification OT - vision-language models COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/12 CRDT- 2026/10/27 10:32 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - bioengineering13101184 [pii] AID - bioengineering-13-01184 [pii] AID - 10.3390/bioengineering13101184 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 12;13(10):1184. doi: 10.3390/bioengineering13101184. PMID- 42939036 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 10 TI - Optimization and Characterization of a Physicochemical Decellularization Protocol for Porcine Tracheal Scaffolds. LID - 10.3390/bioengineering13101181 [doi] LID - 1181 AB - Reconstruction of extensive tracheal defects remains a challenge, and the development of decellularized scaffolds represents a promising strategy for tissue engineering. This study aimed to optimize a porcine tracheal decellularization protocol using sodium deoxycholate (SDC), reducing detergent exposure time without compromising extracellular matrix (ECM) preservation or biomechanical properties. Ten decellularization cycles were evaluated by residual DNA quantification, histological analysis, and residual SDC quantification, and cycles 1, 2, and 10 were selected for ECM characterization, cytocompatibility assessment, and biomechanical analysis. The first cycle was effective for submucosal decellularization, although chondrocytes remained within the tracheal cartilage. ECM architecture and its biomechanical properties were preserved. Additional cycles provided no further benefit to decellularization and were associated with alterations in longitudinal biomechanical parameters. Residual SDC was detected in the scaffolds, and undiluted extracts showed cell viability below 70%. These results indicate that 24 h of SDC exposure achieved effective submucosal decellularization while chondrocytes remained in the cartilage regardless of the number of decellularization cycles. ECM characteristics and tissue biomechanical properties were preserved. FAU - Dos Santos, Elizabete Silva AU - Dos Santos ES AD - Programa de Pos-Graduacao em Cirurgia Toracica e Cardiovascular, Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo 01246-903, SP, Brazil. FAU - da Silva, Ronaldo Soares AU - da Silva RS AD - Laboratorio de Pesquisa em Cirurgia Toracica, Departamento de Cardiopneumologia, Instituto do Coracao (InCor), Hospital das Clinicas HCFMUSP, Universidade de Sao Paulo, Sao Paulo 05403-000, SP, Brazil. FAU - de Morais, Sabrina da Silva AU - de Morais SDS AD - Laboratorio de Pesquisa em Cirurgia Toracica, Departamento de Cardiopneumologia, Instituto do Coracao (InCor), Hospital das Clinicas HCFMUSP, Universidade de Sao Paulo, Sao Paulo 05403-000, SP, Brazil. FAU - da Cruz, Fabiola Matos AU - da Cruz FM AD - Laboratorio de Pesquisa em Cirurgia Toracica, Departamento de Cardiopneumologia, Instituto do Coracao (InCor), Hospital das Clinicas HCFMUSP, Universidade de Sao Paulo, Sao Paulo 05403-000, SP, Brazil. FAU - Kroes, Hector Fugihara AU - Kroes HF AD - Laboratorio de Pesquisa em Cirurgia Toracica, Departamento de Cardiopneumologia, Instituto do Coracao (InCor), Hospital das Clinicas HCFMUSP, Universidade de Sao Paulo, Sao Paulo 05403-000, SP, Brazil. FAU - Maizato, Marina AU - Maizato M AD - Bioengenharia, Instituto do Coracao do Hospital das Clinicas HCFMUSP, Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo 05403-000, SP, Brazil. FAU - Cestari, Idagene Aparecida AU - Cestari IA AUID- ORCID: 0000-0002-4788-8946 AD - Bioengenharia, Instituto do Coracao do Hospital das Clinicas HCFMUSP, Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo 05403-000, SP, Brazil. FAU - Pego-Fernandes, Paulo Manuel AU - Pego-Fernandes PM AD - Laboratorio de Pesquisa em Cirurgia Toracica, Departamento de Cardiopneumologia, Instituto do Coracao (InCor), Hospital das Clinicas HCFMUSP, Universidade de Sao Paulo, Sao Paulo 05403-000, SP, Brazil. FAU - Correia, Aristides Tadeu AU - Correia AT AD - Laboratorio de Pesquisa em Cirurgia Toracica, Departamento de Cardiopneumologia, Instituto do Coracao (InCor), Hospital das Clinicas HCFMUSP, Universidade de Sao Paulo, Sao Paulo 05403-000, SP, Brazil. FAU - Cardoso, Paulo Francisco Guerreiro AU - Cardoso PFG AUID- ORCID: 0000-0003-0749-4165 AD - Laboratorio de Pesquisa em Cirurgia Toracica, Departamento de Cardiopneumologia, Instituto do Coracao (InCor), Hospital das Clinicas HCFMUSP, Universidade de Sao Paulo, Sao Paulo 05403-000, SP, Brazil. LA - eng GR - 88887.647875/2021- 00/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/ PT - Journal Article DEP - 20261010 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701237 OTO - NOTNLM OT - biocompatible materials OT - bioengineering OT - extracellular matrix OT - regenerative medicine OT - tissue engineering OT - trachea OT - tracheal stenosis COIS- The authors declare no conflicts of interest. The funder had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/10 CRDT- 2026/10/27 10:32 PHST- 2026/08/17 00:00 [received] PHST- 2026/09/25 00:00 [revised] PHST- 2026/09/26 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/10 00:00 [pmc-release] AID - bioengineering13101181 [pii] AID - bioengineering-13-01181 [pii] AID - 10.3390/bioengineering13101181 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 10;13(10):1181. doi: 10.3390/bioengineering13101181. PMID- 42939030 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 8 TI - Artificial Intelligence in Healthcare: From Predictive Models to Generative, Multimodal, and Agentic AI. LID - 10.3390/bioengineering13101175 [doi] LID - 1175 AB - Artificial intelligence (AI) is increasingly used across the diagnostic pathway, from screening and disease detection to differential diagnosis, prognostic stratification, and treatment-response assessment. This narrative review examines predictive, generative, multi-modal, and agentic AI through a diagnostic-science lens and evaluates the evidence required for translation into clinical practice. Predictive models can identify complex patterns in images, physiological signals, laboratory data, and electronic health records, but high retrospective accuracy does not establish diagnostic utility. Clinical validity and utility depend on the intended use, target population, disease spectrum and prevalence, reference standard, operating threshold, calibration, and the consequences of false-positive and false-negative results. Generative AI may support problem representation, differential diagnosis, information synthesis, and documentation, yet fluent outputs can omit critical alternatives, amplify false premises, or convey unjustified certainty. Multimodal systems may better reflect clinical reasoning by integrating imaging, text, signals, pathology, and molecular data, but they must demonstrate incremental value over the best single-modality test and remain robust to missing data. Agentic systems can coordinate evidence retrieval, test selection, and sequential workflows, thereby increasing the need for bounded permissions, auditability, error recovery, and human escalation. Across all paradigms, a credible pathway to diagnostic implementation requires independent external and prospective validation, representative consecutive patients, subgroup analysis, workflow and human-AI evaluation, clinical impact studies, and post-deployment surveillance. The field should therefore be judged not by model capability alone, but by whether AI measurably improves diagnostic yield, timeliness, safety, equity, and patient-relevant outcomes in real care settings. FAU - Baydili, Ismail AU - Baydili I AUID- ORCID: 0000-0001-8563-8342 AD - Department of Audiovisual Techniques and Media Production, Vocational School of Technical Sciences, Firat University, Elazig 23119, Turkiye. FAU - Tasci, Burak AU - Tasci B AUID- ORCID: 0000-0002-4490-0946 AD - Vocational School of Technical Sciences, Firat University, Elazig 23119, Turkiye. FAU - Tasci, Gulay AU - Tasci G AUID- ORCID: 0000-0003-2078-0182 AD - Department of Psychiatry, Elazig Fethi Sekin City Hospital, Elazig 23280, Turkiye. FAU - Dogan, Sengul AU - Dogan S AUID- ORCID: 0000-0001-9677-5684 AD - Department of Digital Forensics Engineering, Technology Faculty, Firat University, Elazig 23119, Turkiye. FAU - Tuncer, Turker AU - Tuncer T AUID- ORCID: 0000-0002-5126-6445 AD - Department of Digital Forensics Engineering, Technology Faculty, Firat University, Elazig 23119, Turkiye. LA - eng PT - Journal Article PT - Review DEP - 20261008 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701811 OTO - NOTNLM OT - agentic AI OT - artificial intelligence OT - clinical translation OT - differential diagnosis OT - generative AI OT - medical diagnosis OT - multimodal AI OT - prognostic stratification COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:32 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - bioengineering13101175 [pii] AID - bioengineering-13-01175 [pii] AID - 10.3390/bioengineering13101175 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 8;13(10):1175. doi: 10.3390/bioengineering13101175. PMID- 42939023 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 8 TI - BMA-Net: A Bilateral Attention Network with Retinal Domain Transfer-Learning for CIMT-Based Cardiovascular Risk Classification from Fundus Images. LID - 10.3390/bioengineering13101168 [doi] LID - 1168 AB - Carotid intima-media thickness (CIMT) is an established marker of subclinical atherosclerosis, but its reliance on ultrasound equipment and trained operators limits its suitability for large-scale screening. Retinal fundus photography may offer a more scalable approach to vascular assessment, as it contains retinal vascular features associated with systemic cardiovascular health and can be acquired quickly and non-invasively. This study proposes a two-stage transfer learning framework for CIMT-based cardiovascular risk classification. An attention-enhanced EfficientNet-B4 is first pretrained on the Asia Pacific Tele-Ophthalmology Society 2019 Blindness Detection dataset and subsequently transferred to a weight-sharing Siamese architecture to classify the China Fundus Carotid Intima-Media Thickness dataset. Across ten independent initializations, the proposed model achieved an average macro-F1 score of 77.81% and an area under the receiver operating characteristic curve of 84.08% on the validation set and 78.29% and 85.22%, respectively, on the test set. It achieved overall higher point estimates for performance metrics than those previously reported for the Siamese ResNeXt baseline model with squeeze-and-excitation attention, and demonstrated a reduced disparity in class-wise performance. Grad-CAM analysis further revealed differences in the spatial activation patterns between models trained under different experimental configurations. Overall, these findings support the feasibility of fundus imaging as a scalable and non-invasive approach to CIMT-based cardiovascular risk assessment. FAU - Lyu, Mingze AU - Lyu M AD - Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford OX3 7DQ, UK. FAU - Banerjee, Abhirup AU - Banerjee A AUID- ORCID: 0000-0001-8198-5128 AD - Institute of Biomedical Engineering, Department of Engineering Science, University of Oxford, Oxford OX3 7DQ, UK. LA - eng GR - URF\R1\221314/Royal Society/ GR - RE/24/130024/BHF_/British Heart Foundation/United Kingdom PT - Journal Article DEP - 20261008 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701666 OTO - NOTNLM OT - attention mechanism OT - bilateral image fusion OT - carotid intima-media thickness OT - convolutional neural network OT - retinal fundus imaging COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/08 CRDT- 2026/10/27 10:32 PHST- 2026/09/01 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/08 00:00 [pmc-release] AID - bioengineering13101168 [pii] AID - bioengineering-13-01168 [pii] AID - 10.3390/bioengineering13101168 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 8;13(10):1168. doi: 10.3390/bioengineering13101168. PMID- 42939014 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 3 TI - Designing Protective Systems and Selecting Materials for Motorsport Safety Through Biomechanical Evidence: A Scoping Review. LID - 10.3390/bioengineering13101159 [doi] LID - 1159 AB - Motorsport remains high risk despite advances in crash protection, vehicle design, and medical response. Effective injury prevention requires understanding interactions among crash loading, human injury tolerance, protective-system design, and material behavior. This scoping review searched PubMed, Web of Science, IEEE Xplore, and Scopus for English-language studies from 2000 to July 2026 addressing motorsport safety, injury biomechanics, finite element analysis or modeling, protective-system design, or impact-mitigation materials. Study characteristics were charted, and evidence was narratively synthesized following scoping-review principles. Fifty-six studies were included. Injury prevention increasingly draws on head-kinematic field measurements, injury epidemiology, region-specific finite element analyses, and studies of helmets, restraints, seats, and energy-absorbing structures. The most direct evidence concerns integrated head-neck protection, restraint and seat geometry, and the use of finite-element methods to interpret local injury mechanisms. Emerging material strategies are promising but rely mainly on component-level or simulation-based evidence rather than driver-level injury outcomes. Overall, biomechanics, protective-system design, and material selection are increasingly integrated, but evidence remains uneven. Real-world injury surveillance is limited, simulation studies predominate over field outcomes, and direct evidence linking emerging materials to driver-level injury reduction remains sparse. Progress requires better surveillance datasets, stronger motorsport-specific validation, and more driver-centered protective design. FAU - Zhao, Jiongxiang AU - Zhao J AD - Research Academy of Medicine Combining Sports, Ningbo No. 2 Hospital, Ningbo 315010, China. AD - Faculty of Sports Science, Ningbo University, Ningbo 315211, China. AD - ANTA (China) Co., Ltd., Xiamen 316008, China. AD - Research Center for Molecular Exercise Science, Hungarian University of Sports Science, 1123 Budapest, Hungary. FAU - Shao, Enze AU - Shao E AD - Research Academy of Medicine Combining Sports, Ningbo No. 2 Hospital, Ningbo 315010, China. AD - Faculty of Sports Science, Ningbo University, Ningbo 315211, China. AD - Doctoral School on Safety and Security Sciences, Obuda University, 1088 Budapest, Hungary. FAU - Lu, Zilong AU - Lu Z AD - Research Academy of Medicine Combining Sports, Ningbo No. 2 Hospital, Ningbo 315010, China. AD - Faculty of Sports Science, Ningbo University, Ningbo 315211, China. FAU - Wen, Jun AU - Wen J AD - Research Academy of Medicine Combining Sports, Ningbo No. 2 Hospital, Ningbo 315010, China. AD - Faculty of Sports Science, Ningbo University, Ningbo 315211, China. FAU - Zeng, Chao AU - Zeng C AD - Doctoral School of Materials Sciences and Technologies, Obuda University, 1034 Budapest, Hungary. AD - Institute of Technical Physics and Materials Science, HUN-REN Centre for Energy Research, 1121 Budapest, Hungary. FAU - Goda, Tibor J AU - Goda TJ AD - Doctoral School on Safety and Security Sciences, Obuda University, 1088 Budapest, Hungary. AD - Institute for Natural Sciences and Basic Subjects, Banki Donat Faculty of Mechanical and Safety Engineering, Obuda University, 1088 Budapest, Hungary. FAU - Tafferner-Gulyas, Viktoria AU - Tafferner-Gulyas V AD - John von Neumann Faculty of Informatics, Obuda University, 1034 Budapest, Hungary. FAU - Sun, Dong AU - Sun D AUID- ORCID: 0000-0002-7634-5668 AD - Research Academy of Medicine Combining Sports, Ningbo No. 2 Hospital, Ningbo 315010, China. AD - Faculty of Sports Science, Ningbo University, Ningbo 315211, China. FAU - Gu, Yaodong AU - Gu Y AUID- ORCID: 0000-0003-2187-9440 AD - Research Academy of Medicine Combining Sports, Ningbo No. 2 Hospital, Ningbo 315010, China. AD - Faculty of Sports Science, Ningbo University, Ningbo 315211, China. AD - Research Center for Molecular Exercise Science, Hungarian University of Sports Science, 1123 Budapest, Hungary. LA - eng GR - LR22A020002/Zhejiang Provincial Natural Science Foundation/ GR - 2022Z196/Ningbo key R&D Program/ GR - 2024YFC3607305/Research Academy of Medicine Combining Sports, National Key Research and Development Program of China/ GR - ZKKY2023001/Zhejiang Rehabilitation Medical Association Scientific Research Special Fund/ PT - Journal Article PT - Review DEP - 20261003 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701264 OTO - NOTNLM OT - biomechanics OT - finite element analysis OT - injury prevention OT - motorsport safety OT - protective systems COIS- The authors declare no conflicts of interest. Affiliation 3 reflects the current employment of one author; the company had no involvement in or support for this study. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/03 CRDT- 2026/10/27 10:32 PHST- 2026/08/10 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/30 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - bioengineering13101159 [pii] AID - bioengineering-13-01159 [pii] AID - 10.3390/bioengineering13101159 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 3;13(10):1159. doi: 10.3390/bioengineering13101159. PMID- 42939013 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 3 TI - Periodicity-Driven Adaptive Localization of Respiratory Regions for Non-Contact Respiration Monitoring in Sleeping Infants. LID - 10.3390/bioengineering13101158 [doi] LID - 1158 AB - Respiration is a critical vital sign for assessing the health status of infants during the first year of life. Conventional video-based respiratory monitoring methods often rely on manually defined regions of interest (ROIs) targeting specific anatomical regions, such as the chest or abdomen, or employ pretrained object detection models to localize body regions before extracting respiratory signals. However, in unconstrained sleep environments, infants may adopt diverse sleep postures, making it difficult to assume that respiration-induced movements will consistently appear in the same anatomical region or image location. To address this limitation, we propose an anchor-based respiratory-region localization framework that identifies spatial regions exhibiting prominent respiratory periodicity across the entire video frame without relying on predefined anatomical ROIs or separate body-region localization models. For each grid cell, time-series signals are constructed from inter-frame color/intensity variations and optical-flow-derived motion changes. The magnitude spectra of these signals are then multiplied element-wise to generate a respiratory periodicity map representing the spatial distribution of respiration-related periodicity across the frame. The grid cell with the highest Periodicity Score is selected as the Anchor Grid Cell and used as the primary respiratory region for respiratory-rate estimation. The proposed method was evaluated using 6-fold subject-wise cross-validation on 374 video clips from 15 infants. The single-Anchor Grid Cell approach achieved a mean absolute error (MAE) of 1.9953 breaths per minute (BPM), a root mean square error (RMSE) of 3.9681 BPM, and a Pearson correlation coefficient (PCC) of 0.7287. Moreover, MAEs below 2.5 BPM were obtained for the supine, side-lying, and prone postures, indicating consistent estimation performance across the evaluated sleep postures. These findings suggest that respiration-related information in infants is not necessarily confined to predefined anatomical regions and may instead be expressed at different spatial locations depending on sleep posture. FAU - Jeong, Hyoyeong AU - Jeong H AUID- ORCID: 0009-0006-3218-4184 AD - Department of AI & Informatics, Graduate School, Sangmyung University, Hongjimun 2-gil 20, Jongno-gu, Seoul 03016, Republic of Korea. FAU - Kim, Jinsoo AU - Kim J AD - Department of AI & Informatics, Graduate School, Sangmyung University, Hongjimun 2-gil 20, Jongno-gu, Seoul 03016, Republic of Korea. FAU - Lee, Eui Chul AU - Lee EC AUID- ORCID: 0000-0001-6504-3333 AD - Department of Human-Centered Artificial Intelligence, Graduate School, Sangmyung University, Hongjimun 2-gil 20, Jongno-gu, Seoul 03016, Republic of Korea. LA - eng GR - RS-2026-25529040/Ministry of SMEs and Startups/ PT - Journal Article DEP - 20261003 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701595 OTO - NOTNLM OT - anchor-based localization OT - contactless infant respiration monitoring OT - grid cell OT - periodicity map OT - sleep posture COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/03 CRDT- 2026/10/27 10:32 PHST- 2026/09/08 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/03 00:00 [pmc-release] AID - bioengineering13101158 [pii] AID - bioengineering-13-01158 [pii] AID - 10.3390/bioengineering13101158 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 3;13(10):1158. doi: 10.3390/bioengineering13101158. PMID- 42939010 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 2 TI - Accuracy of Intraocular Lens Power Calculation Formulas for Two Single-Piece Acrylic IOL Models in Eyes with a Shallow Anterior Chamber. LID - 10.3390/bioengineering13101155 [doi] LID - 1155 AB - Introduction: This study evaluated whether the refractive prediction accuracy of conventional and new-generation intraocular lens (IOL) power calculation formulas differs between two single-piece acrylic IOL models in eyes with a shallow anterior chamber depth (ACD). Methods: In total, 100 eyes implanted with Softec HD IOLs (Lenstec) and 102 eyes implanted with SN60WF IOLs (Alcon) were included. All eyes had an ACD of 0.43 D in the Softec group and <0.38 D in the SN60WF group; after optimization, the MAEs were 0.408-0.479 D and 0.334-0.377 D, respectively. Conclusions: The new-generation formulas with ULIB constants showed myopic and hyperopic PEs in the Softec and SN60WF groups, respectively. The SN60WF IOL model demonstrated better refractive prediction accuracy than the Softec model. Formula selection according to IOL model may help improve refractive outcomes in eyes with shallow ACDs. FAU - Wang, Yalan AU - Wang Y AD - Department of Ophthalmology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China. FAU - Lou, Wei AU - Lou W AD - Department of Ophthalmology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China. FAU - Liu, Lu AU - Liu L AD - Department of Ophthalmology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China. FAU - Lu, Xinmin AU - Lu X AD - Department of Ophthalmology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China. FAU - Wu, Qiang AU - Wu Q AD - Department of Ophthalmology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China. FAU - Long, Da AU - Long D AUID- ORCID: 0000-0002-2639-535X AD - Department of Ophthalmology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China. FAU - Hu, Jianyan AU - Hu J AD - Department of Ophthalmology, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China. LA - eng PT - Journal Article DEP - 20261002 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701219 OTO - NOTNLM OT - hydrophilic acrylic OT - hydrophobic acrylic OT - intraocular lens power calculation OT - intraocular lenses OT - shallow anterior chamber COIS- The authors report no conflicts of interest regarding this work. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/02 CRDT- 2026/10/27 10:32 PHST- 2026/09/05 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/02 00:00 [pmc-release] AID - bioengineering13101155 [pii] AID - bioengineering-13-01155 [pii] AID - 10.3390/bioengineering13101155 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 2;13(10):1155. doi: 10.3390/bioengineering13101155. PMID- 42939007 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 2 TI - Interpretable and Calibrated Classification of Clinical Data Using Supervised Feature Binarization. LID - 10.3390/bioengineering13101152 [doi] LID - 1152 AB - Black-box models limit the adoption of artificial intelligence in medicine because their predictions are difficult to interpret and reproduce. We present a statistically grounded framework for interpretable, rule-based clinical classification using the Bernoulli Naive Bayes (BNB) model. Supervised chi-square-guided binarization converts continuous variables into binary indicators by selecting thresholds that maximize association with the clinical outcome within the training folds, which allows BNB to operate on continuous medical data without sacrificing transparency. On three benchmark datasets, Pima Indians Diabetes, Wisconsin Breast Cancer, and Heart Failure Prediction, the framework reached areas under the receiver operating characteristic curve of 0.794, 0.984, and 0.918, respectively. Probabilistic reliability was assessed with a leakage-safe cross-validated calibration analysis reporting Brier score and calibration intercept and slope, and post hoc beta calibration improved probability calibration across datasets. These results indicate that an interpretable, statistically motivated framework can perform comparably to more complex models while providing explicit decision rules expressed in clinical units and risk estimates that are reliable after calibration. A complete worked example further shows that model inference can be reproduced from a printed reference table using only basic arithmetic, without software or proprietary tools, a property that may support auditable use of artificial intelligence in clinical settings. FAU - Garcia, Antony AU - Garcia A AUID- ORCID: 0000-0002-5795-2104 AD - Department of Electrical and Computer Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA. AD - Facultad de Ingenieria Electrica, Universidad Tecnologica de Panama, Panama City 0819-07289, Panama. FAU - Colina, Adrian Noriega de la AU - Colina AN AUID- ORCID: 0000-0001-9933-8164 AD - Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. AD - Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. AD - Department of Neurology and Neurosurgery, McGill University, Montreal, QC H3A 0G4, Canada. AD - Montreal Neurological Institute-Hospital, 3801 University Street, Montreal, QC H3A 2B4, Canada. FAU - Britton, Gabrielle AU - Britton G AUID- ORCID: 0000-0002-1758-2495 AD - Centro de Vacunacion e Investigacion (CEVAXIN), Endpoints Research Sites Network, Panama City 0830-00507, Panama. FAU - Huang, Xinming AU - Huang X AUID- ORCID: 0000-0003-0584-3448 AD - Department of Electrical and Computer Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA. LA - eng PT - Journal Article DEP - 20261002 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701737 OTO - NOTNLM OT - Bernoulli Naive Bayes OT - chi-square thresholding OT - explainable AI OT - feature binarization OT - interpretable machine learning OT - medical classification OT - probability calibration OT - threshold optimization COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/02 CRDT- 2026/10/27 10:32 PHST- 2026/09/16 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/02 00:00 [pmc-release] AID - bioengineering13101152 [pii] AID - bioengineering-13-01152 [pii] AID - 10.3390/bioengineering13101152 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 2;13(10):1152. doi: 10.3390/bioengineering13101152. PMID- 42939006 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Oct 2 TI - Beyond Local Gating: Toward Network-State-Dependent Neuromodulation. LID - 10.3390/bioengineering13101151 [doi] LID - 1151 AB - Brain stimulation is progressively evolving from anatomically targeted interventions toward individualized, adaptive, and network-oriented approaches. Recent developments in brain-state-dependent stimulation have demonstrated that neuromodulation can be optimized by synchronizing stimulation with ongoing neural activity, while adaptive cortico-cortical paired associative stimulation (ccPAS) has extended this principle to distributed cortical networks. However, current approaches continue to define brain state primarily through local electrophysiological features, such as oscillatory phase or power, even though ccPAS is specifically designed to modify communication between interconnected cortical regions. In this opinion paper, we argue that the endogenous variable guiding ccPAS should instead describe the instantaneous physiological state of the targeted cortico-cortical pathway. We propose that online source-space functional connectivity provides a biologically meaningful and technically feasible estimate of this pathway state, thereby enabling a new framework of network-state-dependent neuromodulation. As illustrative applications, we consider pathways spanning different levels of experimental characterization, from established sensorimotor interactions to the less-characterized ventral premotor cortex (PMv)-posterior superior temporal sulcus (pSTS) pathway within the action-observation network (AON). This distinction highlights that the proposed framework is pathway-general, while its experimental implementation requires pathway-specific characterization of stimulation parameters and appropriate physiological readouts. In this framework, resting-state EEG is rapidly reconstructed in source space to estimate pathway connectivity, which is subsequently used to personalize stimulation targets and guide state-dependent ccPAS of a functionally defined cortico-cortical circuit. Finally, we discuss the technological prerequisites, methodological challenges, biological rationale, and open scientific questions that must be addressed before pathway-state-guided neuromodulation can be experimentally validated. We argue that redefining brain state as the instantaneous physiological state of the communication pathway targeted by stimulation may represent the next conceptual step in brain stimulation. FAU - Belardinelli, Paolo AU - Belardinelli P AUID- ORCID: 0000-0002-9290-1804 AD - Department of Psychology and Neuroscience-DIPseN, University of Trento, 38068 Rovereto, TN, Italy. AD - Department of Neurology & Stroke, University of Tubingen, 72076 Tubingen, Germany. AD - Hertie-Institute for Clinical Brain Research, University of Tubingen, 72076 Tubingen, Germany. FAU - Mongiardini, Elena AU - Mongiardini E AUID- ORCID: 0009-0003-4579-6129 AD - Department of Psychology and Neuroscience-DIPseN, University of Trento, 38068 Rovereto, TN, Italy. FAU - Turrini, Sonia AU - Turrini S AUID- ORCID: 0000-0002-4469-9349 AD - Center for Studies and Research in Cognitive Neuroscience, Department of Psychology "Renzo Canestrari", Alma Mater Studiorum Universita di Bologna, Cesena Campus, 47521 Cesena, FC, Italy. FAU - Avenanti, Alessio AU - Avenanti A AUID- ORCID: 0000-0003-1139-9996 AD - Center for Studies and Research in Cognitive Neuroscience, Department of Psychology "Renzo Canestrari", Alma Mater Studiorum Universita di Bologna, Cesena Campus, 47521 Cesena, FC, Italy. AD - Centro de Investigacion en Neuropsicologia y Neurociencias Cognitivas, Universidad Catolica del Maule, Talca 3460000, Chile. LA - eng GR - E63C22003780001/Ministry of Health/ GR - 346/2024/Bial (Portugal)/ PT - Comment PT - Journal Article DEP - 20261002 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701329 OTO - NOTNLM OT - adaptive network-guided neuromodulation OT - cortico-cortical paired associative stimulation (ccPAS) OT - network-state-triggered neuromodulation OT - non-invasive brain stimulation (NIBS) COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:32 MHDA- 2026/10/27 11:33 PMCR- 2026/10/02 CRDT- 2026/10/27 10:32 PHST- 2026/07/25 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:33 [medline] PHST- 2026/10/27 11:32 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/02 00:00 [pmc-release] AID - bioengineering13101151 [pii] AID - bioengineering-13-01151 [pii] AID - 10.3390/bioengineering13101151 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Oct 2;13(10):1151. doi: 10.3390/bioengineering13101151. PMID- 42938997 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Sep 30 TI - Biomechanical Effects of Distal Fragment Translation Guided by Target Postoperative Intermetatarsal Angle in Minimally Invasive Transverse Osteotomy for Hallux Valgus: A Patient-Specific Finite Element Study. LID - 10.3390/bioengineering13101141 [doi] LID - 1141 AB - This study investigated the biomechanical effects of distal fragment translation guided by target postoperative first-second intermetatarsal angle (IMA) in minimally invasive transverse osteotomy (MITO) for hallux valgus using patient-specific finite element models. Three-dimensional models were reconstructed from computed tomography data of three female patients representing mild, moderate, and severe hallux valgus. The distal first-metatarsal fragment was translated laterally to achieve target postoperative IMA values of 9 degrees , 8 degrees , 7 degrees , 6 degrees , and 5 degrees . Maximum von Mises stresses of the first through fifth metatarsals and Kirschner wires, together with mean osteotomy contact pressure and contact area, were evaluated under standardized static standing. As the target IMA decreased, the required translation increased, osteotomy contact area decreased, and mean contact pressure increased in all models. First-metatarsal stress increased most markedly in the severe model, reaching 25.74 MPa at 5 degrees . In the mild and moderate models, further correction at the smaller IMA settings produced relatively limited additional changes in metatarsal stress while fixation stress and osteotomy-interface demands continued to increase. In contrast, the severe model showed continued increases in first-metatarsal and fixation stresses with further correction. These exploratory findings indicate that the mechanical effects of correction depend on both the target postoperative IMA and the distal fragment translation required to achieve it. FAU - Shi, Jiaming AU - Shi J AUID- ORCID: 0009-0005-5443-6066 AD - Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, No. 6 Jiefang Street, Dalian 116001, China. AD - The First Clinical College, Dalian Medical University, Dalian 116011, China. FAU - Jiang, Yongze AU - Jiang Y AD - Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, No. 6 Jiefang Street, Dalian 116001, China. FAU - Xiong, Wanqi AU - Xiong W AD - Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, No. 6 Jiefang Street, Dalian 116001, China. FAU - Zhang, Tao AU - Zhang T AD - Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, No. 6 Jiefang Street, Dalian 116001, China. AD - The First Clinical College, Dalian Medical University, Dalian 116011, China. FAU - Dong, Yujin AU - Dong Y AD - Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, No. 6 Jiefang Street, Dalian 116001, China. FAU - Dong, Xinli AU - Dong X AD - Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, No. 6 Jiefang Street, Dalian 116001, China. FAU - Liu, Jiahe AU - Liu J AD - Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, No. 6 Jiefang Street, Dalian 116001, China. FAU - Liu, Baoyi AU - Liu B AUID- ORCID: 0000-0003-3531-415X AD - Department of Orthopedics, Affiliated Zhongshan Hospital of Dalian University, No. 6 Jiefang Street, Dalian 116001, China. LA - eng GR - 2023RQ034/Dalian Science and Technology Bureau/ GR - DLUXK-2025-QN-008/Dalian University/ GR - NHWPRleft broken bracket2024right broken bracket41/National Health Commission of the People's Republic of China/ GR - 2024JH2/102600062/Department of Science and Technology of Liaoning Province/ GR - CACM-2025-GSKY03-B5/China Association of Chinese Medicine/ GR - YXMJ-QN-21/Liaoning Provincial Health Commission/ PT - Journal Article DEP - 20260930 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701776 OTO - NOTNLM OT - Kirschner wire OT - distal fragment translation OT - finite element analysis OT - hallux valgus OT - intermetatarsal angle OT - minimally invasive transverse osteotomy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/09/30 CRDT- 2026/10/27 10:32 PHST- 2026/09/12 00:00 [received] PHST- 2026/09/27 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - bioengineering13101141 [pii] AID - bioengineering-13-01141 [pii] AID - 10.3390/bioengineering13101141 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Sep 30;13(10):1141. doi: 10.3390/bioengineering13101141. PMID- 42938995 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Sep 30 TI - Extracellular Vesicle Communication in the Central Nervous System: Mechanisms, Disease States, and Therapeutic Opportunities. LID - 10.3390/bioengineering13101140 [doi] LID - 1140 AB - Extracellular vesicles (EVs) are increasingly recognized as regulated mediators of intercellular communication in the central nervous system (CNS), transferring proteins, lipids, metabolites, and nucleic acids among neurons, glia, and other cells. EV communication comprises a complex integrated pathway that includes vesicle biogenesis, selective cargo sorting, secretion, extracellular transport, target-cell recognition, uptake, intracellular trafficking, functional cargo delivery, and recipient-cell responses. These processes are controlled by membrane trafficking machinery, cytoskeletal dynamics, lipid metabolism, calcium signaling, and the endosomal-lysosomal system and are further shaped by the physiological and pathophysiological states of donor and recipient cells. Genetic variation, proteotoxic stress, inflammation, metabolic and redox alterations, neuronal activity, and endolysosomal dysfunction can therefore affect multiple stages of the EV communication pathway in neurodevelopmental and neurodegenerative disorders. EV alterations reflect dynamic states rather than fixed disease signatures, with effects depending on cellular origin, EV subtype, anatomical context, developmental or disease state, and recipient-cell competence. This review examines CNS EV physiology and proposes a state-dependent framework for biomarkers and therapies, distinguishing EV detection from functional cargo delivery, cross-sectional associations from longitudinal progression, and cellular uptake from intracellular target engagement. FAU - Bi, Xiaoning AU - Bi X AUID- ORCID: 0000-0001-7449-7003 AD - College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA 91766, USA. FAU - Gootin, Hanna AU - Gootin H AD - College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA 91766, USA. FAU - Su, Wenyue AU - Su W AD - College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, Pomona, CA 91766, USA. FAU - Baudry, Michel AU - Baudry M AD - College of Dental Medicine, Western University of Health Sciences, Pomona, CA 91766, USA. LA - eng GR - R15 MH101703/MH/NIMH NIH HHS/United States GR - 2R15MH101703-04A1/NH/NIH HHS/United States PT - Journal Article PT - Review DEP - 20260930 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701430 OTO - NOTNLM OT - EV biomarkers OT - EV communication OT - EV therapeutics OT - central nervous system OT - endolysosomal system OT - extracellular vesicles OT - neurodegenerative diseases OT - neurodevelopmental disorders OT - state-dependent signaling COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/09/30 CRDT- 2026/10/27 10:32 PHST- 2026/08/31 00:00 [received] PHST- 2026/09/21 00:00 [revised] PHST- 2026/09/29 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/09/30 00:00 [pmc-release] AID - bioengineering13101140 [pii] AID - bioengineering-13-01140 [pii] AID - 10.3390/bioengineering13101140 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Sep 30;13(10):1140. doi: 10.3390/bioengineering13101140. PMID- 42938993 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Sep 29 TI - End-Effector Robotic Rehabilitation After Spinal Cord Injury: A Systematic Review of Devices, Operational Characteristics Used, Outcome Measures, and Rehabilitation Effectiveness. LID - 10.3390/bioengineering13101138 [doi] LID - 1138 AB - BACKGROUND: End-effector robotic devices are increasingly used in rehabilitation to retrain neural pathways through repetitive, task-specific exercise. However, the influence of movement kinematic variables remains unclear. This systematic review aimed to; (i) identify end-effector robotic systems used in upper- and lower-limb spinal cord injury rehabilitation, (ii) describe their technical specifications and operational use, and (iii) explore associations between device use characteristics and study outcomes. METHODS: A systematic review of five databases was conducted from inception to June 2026. RESULTS: Fifteen studies, ten lower-limb and five upper-limb, met the inclusion criteria. These covered five commercially available lower-limb devices and three upper-limb devices. Most lower-limb studies demonstrated moderate-to-large improvements in balance, ambulation (WISCI-II), walking endurance (6mWT), and independence (SCIM-III). End-effector training produced comparable outcomes to both conventional therapy and exoskeleton-based rehabilitation in controlled comparisons. Upper-limb studies showed substantial heterogeneity in outcome selection, reflecting diverse rehabilitation goals. CONCLUSIONS: WISCI-II and 10MWT were the most used lower-limb functional assessments, with SCIM-III used to assess independence for both upper- and lower-limb motor impairments. Reporting of kinematic settings are often limited and concentrated at study onset, limiting assessment of their contribution to outcomes. Reporting the progression of session-level metrics is needed to better understand how time-varying kinematics affect intervention effectiveness. FAU - Baldock, Michael AU - Baldock M AUID- ORCID: 0000-0002-7678-8549 AD - School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK. FAU - Caggiari, Silvia AU - Caggiari S AUID- ORCID: 0000-0002-8928-2141 AD - School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK. AD - Department of Mechanical Engineering, School of Engineering, University of Southampton, Southampton SO17 1BJ, UK. FAU - Capozio, Antonio AU - Capozio A AD - School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK. AD - Department of Psychology, Faculty of Arts and Sciences, Edge Hill University, Ormskirk L39 4QP, UK. FAU - Lunn, David E AU - Lunn DE AD - Carnegie School of Sport, Leeds Beckett University, Leeds LS6 3QS, UK. FAU - Gawne, Frances AU - Gawne F AD - School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK. FAU - Delis, Ioannis AU - Delis I AUID- ORCID: 0000-0001-8940-5036 AD - School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK. FAU - Astill, Sarah L AU - Astill SL AUID- ORCID: 0000-0002-9443-6934 AD - School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT, UK. LA - eng GR - Nialls Foundation/ PT - Journal Article PT - Review DEP - 20260929 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701345 OTO - NOTNLM OT - end-effector OT - kinematics OT - lower-limb OT - rehabilitation OT - spinal cord injury OT - upper-limb COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/09/29 CRDT- 2026/10/27 10:32 PHST- 2026/08/06 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/18 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/09/29 00:00 [pmc-release] AID - bioengineering13101138 [pii] AID - bioengineering-13-01138 [pii] AID - 10.3390/bioengineering13101138 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Sep 29;13(10):1138. doi: 10.3390/bioengineering13101138. PMID- 42938991 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Sep 28 TI - Multi-Parameter Genomic Evolution of SARS-CoV-2 Across Ten US States During the Vaccination Era, 2020-2025. LID - 10.3390/bioengineering13101136 [doi] LID - 1136 AB - SARS-CoV-2 has adapted continuously since 2019, yet no multi-parameter framework has characterized this trajectory across geographically distinct United States populations. We present a systematic 13-parameter analysis of 137,059 high-quality genomes from ten diverse US states across three phases: pre-vaccination (January-December 2020), early vaccination (January-June 2021), and sustained immune pressure (July 2021-December 2025), the latter dominated by the high-volume Delta period with an early-Omicron contribution. Approximately 5000 genomes were retrieved per state per phase from GISAID EpiCoV under stringent quality filters, and each phase dataset was partitioned into three subsets, giving 90 subsamples in total, to quantify subsampling stability. Parameters spanned genome-wide mutation rate, dN/dS and Ti/Tv, site-specific selection (HyPhy FUBAR), spike and ACE2 contact-residue profiling, insertion-deletion characterization, furin cleavage site evolution, N-linked glycosylation dynamics, lineage-mutation concordance, amino acid physicochemical shifts, and protein-wise dN/dS ranking and mutation load. Genome-wide mutation rate rose monotonically in all ten states, from 0.050% to 0.149%, and dN/dS rose from 0.611 to 1.103, crossing neutrality in nine of ten states by Phase 3. Amino acid mutation load increased 3.95-fold, from 9.12 to 36.03 substitutions per sequence, while Ti/Tv fell from 3.69 to 2.11. Spike position 142 was diversifying in all ten states in Phase 3, with positions 484, 452, and 478 recurring in 70, 60, and 60 percent of states. ORF8 followed a spike-then-collapse trajectory (dN/dS 3.43, 14.23, 1.01), consistent with progressive loss of function. The reproducibility of these effects across ten geographically distinct state populations establishes a state-level baseline for SARS-CoV-2 immune escape. FAU - Thomas, Som V AU - Thomas SV AUID- ORCID: 0000-0001-9182-4002 AD - Department of Biomedical Engineering, University of Cincinnati, Cincinnati, OH 45267, USA. FAU - Chen, Jing AU - Chen J AUID- ORCID: 0000-0002-6227-7393 AD - Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA. FAU - Qian, Dong AU - Qian D AUID- ORCID: 0000-0001-9367-0924 AD - Department of Mechanical Engineering, The University of Texas at Dallas, Richardson, TX 75080, USA. FAU - Spearman, Paul AU - Spearman P AUID- ORCID: 0000-0001-5393-9511 AD - Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA. FAU - Shi, Donglu AU - Shi D AUID- ORCID: 0000-0002-0837-7780 AD - Department of Biomedical Engineering, University of Cincinnati, Cincinnati, OH 45267, USA. AD - The Materials Science and Engineering Program, University of Cincinnati, Cincinnati, OH 45267, USA. LA - eng GR - 2029268/U.S. National Science Foundation/ PT - Journal Article DEP - 20260928 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13702006 OTO - NOTNLM OT - GISAID OT - SARS-CoV-2 OT - dN/dS OT - genomic surveillance OT - immune escape OT - molecular evolution OT - positive selection OT - spike glycoprotein COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/09/28 CRDT- 2026/10/27 10:32 PHST- 2026/08/20 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - bioengineering13101136 [pii] AID - bioengineering-13-01136 [pii] AID - 10.3390/bioengineering13101136 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Sep 28;13(10):1136. doi: 10.3390/bioengineering13101136. PMID- 42938988 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Sep 28 TI - Deep Learning Approaches for Classification of Honeycombing and Ground Glass Opacity in Lung CT Imaging: A Systematic Review. LID - 10.3390/bioengineering13101134 [doi] LID - 1134 AB - Lung diseases such as Honeycombing (HC) and Ground Glass Opacity (GGO) present significant challenges in medical image analysis due to their overlapping visual characteristics and complex structural patterns in Computed Tomography (CT) images. These conditions often exhibit similar appearances to normal lung tissue, making accurate differentiation difficult and highly dependent on imaging quality and expert interpretation. This paper presents a systematic literature review of studies published between 2011 and 2026 on lung disease detection and classification using medical imaging. A total of 98 selected studies is analyzed to examine recent developments in Machine Learning (ML) and Deep Learning (DL) techniques for automated lung disease analysis. The review covers key aspects including imaging modalities such as CT and chest X-ray, publicly available and private datasets, preprocessing techniques, feature extraction methods, classification models, and evaluation metrics. The findings indicate that traditional machine learning approaches were widely used in earlier studies, while recent research has increasingly shifted towards deep learning methods due to their ability to automatically learn discriminative features from medical images. Among these approaches, Convolutional Neural Networks (CNNs), ResNet-based architectures, DenseNet, and EfficientNet are the most commonly adopted models for lung disease classification. Despite significant advancements, several challenges remain, including limited availability of publicly accessible datasets, class imbalance, variability in imaging protocols, and lack of standardized evaluation frameworks. In addition, misclassification between visually similar conditions such as Honeycombing and GGO remains a persistent issue that affects diagnostic reliability. The review identifies key research gaps and highlights the need for standardized datasets, improved preprocessing techniques, and consistent evaluation protocols. Future research should focus on enhancing model generalisation and robustness across diverse datasets to support reliable automated lung disease diagnosis in clinical practice. FAU - Sithiq, Hairul Aysa Abdul Halim AU - Sithiq HAAH AD - Department of Information Systems, Faculty of Computer Science and Information Technology, University of Malaya, Kuala Lumpur 50603, Malaysia. FAU - Shuib, Liyana AU - Shuib L AUID- ORCID: 0000-0002-7907-0671 AD - Department of Information Systems, Faculty of Computer Science and Information Technology, University of Malaya, Kuala Lumpur 50603, Malaysia. FAU - Ahmad, Muneer AU - Ahmad M AUID- ORCID: 0000-0001-5047-1108 AD - Department of Computing, University of Roehampton, Roehampton Lane, London SW15 5PH, UK. FAU - Antony, Chermaine Deepa AU - Antony CD AD - Department of Biomedical Imaging, University Malaya Medical Centre, Kuala Lumpur 50603, Malaysia. FAU - Iftikhar, Mohsin AU - Iftikhar M AD - Faculty of Computer Information Science, Higher Colleges of Technology, Al-Faseel Campus, Fujairah P.O. Box 1626, United Arab Emirates. FAU - Shah, Peer Azmat AU - Shah PA AUID- ORCID: 0000-0001-7591-1704 AD - Faculty of Computer Information Science, Higher Colleges of Technology, Al-Faseel Campus, Fujairah P.O. Box 1626, United Arab Emirates. LA - eng PT - Journal Article PT - Review DEP - 20260928 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701413 OTO - NOTNLM OT - CT imaging OT - Resnet50v2 OT - convolutional neural networks OT - deep learning OT - ground-glass opacity OT - honeycombing lungs OT - lung disease detection OT - medical image classification COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/09/28 CRDT- 2026/10/27 10:32 PHST- 2026/04/07 00:00 [received] PHST- 2026/05/25 00:00 [revised] PHST- 2026/09/15 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - bioengineering13101134 [pii] AID - bioengineering-13-01134 [pii] AID - 10.3390/bioengineering13101134 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Sep 28;13(10):1134. doi: 10.3390/bioengineering13101134. PMID- 42938987 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Sep 28 TI - Artificial Intelligence Algorithms for Early Detection and Risk Stratification of Type 2 Diabetes Mellitus, Multimodal Bioinformatic Phenotyping, and Its Complications: A Technical Review of Classical Machine Learning, Deep Learning, and Post-Hoc Feature Attribution. LID - 10.3390/bioengineering13101132 [doi] LID - 1132 AB - Type 2 diabetes mellitus (T2DM) affects hundreds of millions of people worldwide, and nearly half of all cases remain undiagnosed. This technical review, conducted under conducted under the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and structured around the Population, Intervention, Comparison, Outcome and Context (PICOC) framework, identifies and synthesises the evidence on artificial intelligence (AI) algorithms for the early detection of T2DM and the risk stratification of its complications, evaluating their performance against conventional diagnostic methods and characterising their clinical implementation contexts. A systematic search in Scopus with a four-stage screening process, synthesised across five research dimensions, yielded 255 records, of which 47 studies met the inclusion criteria (2025-2026). Ensemble methods based on gradient boosting, particularly particularly extreme gradient boosting (XGBoost), emerged as the dominant primary modelling approach, while logistic regression was the most frequently reported algorithm, predominantly as a baseline comparator, with area under the receiver operating characteristic curve (AUC-ROC) values ranging from 0.63 to 0.97. AI models consistently outperformed glycated haemoglobin (HbA1c), whose reported sensitivity for prediabetes was only 49%, reaching accuracies of up to 96.7%, although that upper figure comes from a narrowly defined cohort with a high baseline prevalence and does not represent general screening performance. Multimodal approaches achieved the highest discrimination, combining wearables, continuous glucose monitoring, and genomics (AUC 0.96 internal, 0.90 external, for the separation of self-reported glycaemic states), and a real-world clinical deployment integrated into electronic health records improved glycaemic control outcomes among high-risk patients. An assessment of the eight anchor studies with thePrediction model Risk Of Bias ASsessment Tool updated for AI (PROBAST + AI) found high concern regarding quality in six of them, driven mainly by cross-sectional designs and self-reported outcomes. Overall, AI stands out as a viable and scalable complement to conventional screening, although gaps remain in multicentre prospective validation, reporting standardisation, and algorithmic equity across populations. FAU - Orozco-Romero, Kattia AU - Orozco-Romero K AUID- ORCID: 0009-0002-4560-5209 AD - Universidad Tecnologica del Peru, Lima 15046, Peru. FAU - Pariona-Torres, Jonas AU - Pariona-Torres J AUID- ORCID: 0000-0002-8181-2283 AD - Universidad Tecnologica del Peru, Lima 15046, Peru. FAU - Cornejo, Jose AU - Cornejo J AUID- ORCID: 0000-0003-4096-9337 AD - Universidad Tecnologica del Peru, Lima 15046, Peru. LA - eng PT - Journal Article PT - Review DEP - 20260928 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701371 OTO - NOTNLM OT - artificial intelligence OT - deep learning OT - diabetes prediction OT - diabetic complications OT - early detection OT - feature selection OT - machine learning OT - predictive models OT - risk stratification COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/09/28 CRDT- 2026/10/27 10:32 PHST- 2026/08/01 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/09/28 00:00 [pmc-release] AID - bioengineering13101132 [pii] AID - bioengineering-13-01132 [pii] AID - 10.3390/bioengineering13101132 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Sep 28;13(10):1132. doi: 10.3390/bioengineering13101132. PMID- 42938984 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Sep 27 TI - Intelligent and Energy-Autonomous Wearable and Implantable Biosensors: Nanomaterial Interfaces, Energy Harvesting, Edge AI, and Long-Term Reliability. LID - 10.3390/bioengineering13101129 [doi] LID - 1129 AB - Wearable and implantable biosensors are becoming small distributed biomedical systems rather than isolated transducers. Their practical performance depends on how the sensing interface, analog front end, power source, local computation, wireless link, packaging, and therapeutic output interact over time. The analysis focuses on that cross-layer problem, with emphasis on nanomaterial interfaces, energy autonomy, edge intelligence, and long-term reliability. Graphene, carbon nanotubes, MXenes, and transition-metal dichalcogenides are discussed across electrochemical, field-effect, impedance, optical, and radio-frequency transduction. Mechanical nanogenerators, biofuel cells, wireless power transfer, and hybrid storage are compared using the energy actually available after rectification and regulation rather than peak generator output alone. Quantitative re-plots illustrate non-monotonic carbon-nanotube loading in triboelectric layers and voltage-tunable few-layer-graphene microwave components. Edge AI is treated as part of the power and measurement architecture: local inference can reduce radio traffic and latency, but introduces model drift, uncertainty, and update requirements. The final sections connect biofouling, encapsulation, mechanical fatigue, calibration drift, wireless safety, and algorithm lifecycle to a common validation ladder for wearable, insertable, and implantable systems. FAU - Bellucci, Stefano AU - Bellucci S AUID- ORCID: 0000-0003-0326-6368 AD - National Institute of Materials Physics, Atomistilor 405A, 077125 Magurele, Romania. AD - Qi S.r.l., Via Monte d'Oro 2/A, 00071 Pomezia, RM, Italy. AD - Universidad Ecotec, Km 13.5 Samborondon, Samborondon 092302, Ecuador. LA - eng GR - FISA-2023-00229/Ministero dell'universita e della ricerca/ PT - Journal Article PT - Review DEP - 20260927 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701389 OTO - NOTNLM OT - bioelectronics OT - edge AI OT - implantable biosensors OT - nanomaterials OT - neuromorphic computing OT - reliability OT - self-powered sensing OT - triboelectric nanogenerator OT - wearable biosensors OT - wireless power transfer COIS- The author is affiliated with Qi S.r.l. This affiliation did not influence the selection or interpretation of the literature. The author declares no other competing interests. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/09/27 CRDT- 2026/10/27 10:32 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/09/25 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - bioengineering13101129 [pii] AID - bioengineering-13-01129 [pii] AID - 10.3390/bioengineering13101129 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Sep 27;13(10):1129. doi: 10.3390/bioengineering13101129. PMID- 42938982 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Sep 27 TI - Clinical-Radiomics Nomogram Based on (18)F-FDG PET/CT for Distinguishing Lymphoma from Cancer of Unknown Primary in Head and Neck Metastatic Poorly Differentiated Cancer. LID - 10.3390/bioengineering13101127 [doi] LID - 1127 AB - OBJECTIVE: To develop and validate a clinical-radiomics nomogram based on (18)F-FDG PET/CT for differentiating lymphoma from cancer of unknown primary (CUP) in head and neck metastatic poorly differentiated cancer. METHODS: This retrospective study enrolled 192 patients, including 151 CUP and 41 lymphoma cases. Patients were randomly divided into a training (n = 134, 70%) and test cohorts (n = 58, 30%) using a fixed random seed for partitioning. Radiomic features were separately extracted from PET/CT images, and feature selection was performed using the least absolute shrinkage and selection operator algorithm. The optimal radiomics model was constructed via ten-fold cross-validation and comparative modeling with multiple machine learning methods. The clinical-radiomics nomogram was established by combining the radiomics model score and the clinical model score derived from gender and the metabolic heterogeneity index. The class distribution was preserved to reflect real-world clinical prevalence, consistent with recommendations for clinical prediction models. RESULTS: The lymphoma group had a significantly higher female proportion (53.7%) than the CUP group (17.9%). The radiomics model yielded areas under the curve (AUC) of 0.916 in the training cohort and 0.812 in the test cohort. The final clinical-radiomics nomogram achieved higher and more robust performance, with AUCs of 0.924 (95% CI: 0.868-0.980) in the training set and 0.912 (95% CI: 0.837-0.987) in the test set, respectively. The standalone radiomics model exhibited a drop in sensitivity when evaluated in the test cohort, which directly demonstrates the incremental predictive value of integrating well-selected clinical parameters into the final model. CONCLUSIONS: The clinical-radiomics nomogram shows promising potential for differentiating lymphoma from CUP in poorly differentiated cervical lymph node malignancies. As a preliminary, single-center study, these findings warrant further validation in larger, multi-center cohorts before clinical implementation. FAU - Chen, Wen AU - Chen W AUID- ORCID: 0000-0001-8013-0478 AD - Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, Fudan University, Shanghai 200032, China. AD - College of Biomedical Engineering, Yiwu Research Institute, Fudan University, Shanghai 200433, China. AD - Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China. AD - Center for Biomedical Imaging, Fudan University, Shanghai 200032, China. AD - Shanghai Engineering Research Center of Molecular Imaging Probes, Shanghai 200032, China. AD - Key Laboratory of Nuclear Physics and Ion-Beam Application (MOE), Fudan University, Shanghai 200433, China. FAU - Xu, Mingzhen AU - Xu M AUID- ORCID: 0000-0003-4011-8495 AD - Department of Nuclear Medicine, Huadong Hospital Affiliated to Fudan University, Shanghai 200040, China. FAU - Gu, Bingxin AU - Gu B AUID- ORCID: 0000-0001-8813-836X AD - Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, Fudan University, Shanghai 200032, China. AD - Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China. AD - Center for Biomedical Imaging, Fudan University, Shanghai 200032, China. AD - Shanghai Engineering Research Center of Molecular Imaging Probes, Shanghai 200032, China. AD - Key Laboratory of Nuclear Physics and Ion-Beam Application (MOE), Fudan University, Shanghai 200433, China. FAU - Zhang, Jianping AU - Zhang J AD - Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, Fudan University, Shanghai 200032, China. AD - Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China. AD - Center for Biomedical Imaging, Fudan University, Shanghai 200032, China. AD - Shanghai Engineering Research Center of Molecular Imaging Probes, Shanghai 200032, China. AD - Key Laboratory of Nuclear Physics and Ion-Beam Application (MOE), Fudan University, Shanghai 200433, China. FAU - Shi, Xiaoqiang AU - Shi X AD - Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, Fudan University, Shanghai 200032, China. AD - Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China. AD - Center for Biomedical Imaging, Fudan University, Shanghai 200032, China. AD - Shanghai Engineering Research Center of Molecular Imaging Probes, Shanghai 200032, China. AD - Key Laboratory of Nuclear Physics and Ion-Beam Application (MOE), Fudan University, Shanghai 200433, China. FAU - Zhang, Ni AU - Zhang N AD - Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, Fudan University, Shanghai 200032, China. AD - Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China. AD - Center for Biomedical Imaging, Fudan University, Shanghai 200032, China. AD - Shanghai Engineering Research Center of Molecular Imaging Probes, Shanghai 200032, China. AD - Key Laboratory of Nuclear Physics and Ion-Beam Application (MOE), Fudan University, Shanghai 200433, China. FAU - Song, Shaoli AU - Song S AUID- ORCID: 0000-0003-2544-7522 AD - Department of Nuclear Medicine, Fudan University Shanghai Cancer Center, Fudan University, Shanghai 200032, China. AD - Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China. AD - Center for Biomedical Imaging, Fudan University, Shanghai 200032, China. AD - Shanghai Engineering Research Center of Molecular Imaging Probes, Shanghai 200032, China. AD - Key Laboratory of Nuclear Physics and Ion-Beam Application (MOE), Fudan University, Shanghai 200433, China. LA - eng GR - XTCX-CY-2025-017/Shanghai Collaborative Innovation Project/ GR - HCXBCY-2024-001/Shanghai Collaborative Innovation Project/ GR - HNKFHBZ202319(36)/Nuclear Technology R&D Program/ GR - SSF828085/Shanghai Oriental Talents Program Youth Project/ GR - 23TS1400800/Shanghai Science and Technology Commission Project/ PT - Journal Article DEP - 20260927 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701412 OTO - NOTNLM OT - PET/CT OT - cancer of unknown primary OT - heterogeneity index OT - lymphoma OT - machine learning OT - nomogram OT - radiomics COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/09/27 CRDT- 2026/10/27 10:32 PHST- 2026/08/13 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/24 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/09/27 00:00 [pmc-release] AID - bioengineering13101127 [pii] AID - bioengineering-13-01127 [pii] AID - 10.3390/bioengineering13101127 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Sep 27;13(10):1127. doi: 10.3390/bioengineering13101127. PMID- 42938980 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Sep 26 TI - Interferential Current Therapy and Spray-And-Stretch in Musculoskeletal Disorders: A Narrative Review of Mechanisms, Evidence, and Clinical Positioning in the Absence of Direct Comparative Trials. LID - 10.3390/bioengineering13101125 [doi] LID - 1125 AB - BACKGROUND: Interferential current therapy (ICT) and spray-and-stretch are two low-cost, non-invasive modalities that coexist on the treatment menus of outpatient musculoskeletal clinics, yet no published study has directly compared them, and no guidance document provides principled criteria for choosing between them. OBJECTIVE: To characterise the mechanistic basis, clinical evidence, safety profile, and practical implementation of each modality across the full range of musculoskeletal disorders, and to propose a hypothesis-generating framework for modality positioning in the absence of direct comparative trials. METHODS: A narrative review of literature retrieved from PubMed/MEDLINE, Embase, Scopus, the Cochrane Library, PEDro, and Google Scholar from database inception to 1 August 2026, using the search logic (musculoskeletal condition terms) AND (ICT terms OR spray-and-stretch terms), supplemented by hand-searching. The combined search retrieved 3241 records across six databases; 46 primary studies were included after two-reviewer sequential screening. Risk of bias was assessed using the PEDro scale (RCTs) and the Downs and Black checklist (observational studies). No quantitative synthesis was performed. RESULTS: For ICT, multiple randomised trials and meta-analyses across at least four anatomical regions show that stand-alone ICT produces a small and inconsistent analgesic effect versus sham, whereas ICT as a co-intervention yields modest short-term improvements in pain and disability in chronic non-specific low back pain and knee osteoarthritis. For spray-and-stretch, a small number of predominantly fair-quality RCTs, almost all limited to the upper trapezius, show a real but brief effect of the combined procedure; the independent contribution of the vapocoolant has not been isolated in any dismantling study. CONCLUSIONS: The two modalities address mechanistically distinct clinical problems and are not directly comparable on the current evidence. We propose a hypothesis-generating positioning framework-unvalidated and intended to guide future comparative trials rather than to serve as a clinical decision rule-in which ICT is considered for deep, regional, multi-session analgesic adjunction, and spray-and-stretch for single-session restoration of range of motion around a discrete taut band. Direct comparative trial data are needed to test this framework. FAU - Jung, Chul Hee AU - Jung CH AUID- ORCID: 0000-0002-1965-7315 AD - Department of Orthopaedic Surgery, Aerospace Medical Center, Republic of Korea Air Force, Cheongju 28187, Republic of Korea. FAU - Choi, Seok Yeon AU - Choi SY AUID- ORCID: 0000-0003-4720-4580 AD - Department of Rehabilitation Medicine, Aerospace Medical Center, Republic of Korea Air Force, Cheongju 28187, Republic of Korea. FAU - Lee, Dong Ha AU - Lee DH AUID- ORCID: 0000-0001-7826-2148 AD - Department of Orthopaedic Surgery, Aerospace Medical Center, Republic of Korea Air Force, Cheongju 28187, Republic of Korea. AD - Department of Orthopaedic Surgery, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu, Seoul 03080, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20260926 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701244 OTO - NOTNLM OT - cryotherapy OT - electric stimulation therapy OT - evidence-based medicine OT - musculoskeletal pain OT - myofascial pain syndromes OT - pain management OT - physical therapy modalities OT - trigger points COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/09/26 CRDT- 2026/10/27 10:32 PHST- 2026/08/21 00:00 [received] PHST- 2026/09/22 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/09/26 00:00 [pmc-release] AID - bioengineering13101125 [pii] AID - bioengineering-13-01125 [pii] AID - 10.3390/bioengineering13101125 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Sep 26;13(10):1125. doi: 10.3390/bioengineering13101125. PMID- 42938975 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Sep 25 TI - Laser-Fabricated APTES-Functionalized Interdigitated Electrodes with a Portable Readout: A Proof-of-Concept Impedimetric Response to Aqueous Nosema Spore Suspensions. LID - 10.3390/bioengineering13101120 [doi] LID - 1120 AB - Nosemosis, caused by the microsporidian parasites Nosema apis and Nosema ceranae, is a major contributor to honeybee (Apis mellifera) colony losses, yet its diagnosis still relies on destructive sampling followed by light microscopy or nucleic-acid amplification in a laboratory. We describe an impedimetric transducer intended as a first step toward a non-destructive alternative. Interdigitated gold electrodes were patterned on standard microscope slides by nanosecond laser ablation, a route that requires neither photolithography nor cleanroom processing, and were functionalized with (3-aminopropyl)triethoxysilane (APTES) deposited from either ethanol or toluene. Because the 0.01-50 MHz measurement window lies far above the range in which faradaic charge transfer contributes measurably, the response is non-faradaic and is governed by the permittivity and the ionic conductivity of the deposited droplet rather than by interfacial charge transfer. Films deposited from ethanol gave the largest and most reproducible contrast between an aqueous spore suspension (1500 spores/microL) and a deionized-water control. At the structural resonance of the electrode ( approximately 16 MHz) the contrast in impedance magnitude is approximately 10% and is not resolved above the chip-to-chip scatter, which is expected because the magnitude at resonance is set by the series resistance of the electrode path rather than by the droplet. The two liquids are instead separated by the reactive component: the imaginary part of the impedance differs by 15.7 +/- 1.8 MOmega near 6.8 MHz, about nine times the replicate uncertainty, and the phase by 33 +/- 7 degrees at 4.5 MHz. A self-contained readout built around an ESP32 microcontroller reproduced the direction of this change, its output voltage rising by approximately 33% after the suspension was applied. The scope of these observations is deliberately narrow: a single spore concentration in deionized water, no spore-free filtrate control, no calibration series, no selectivity panel and no measurement in a fecal matrix. The contribution is accordingly methodological-a cleanroom-free fabrication route, a quantified solvent dependence of the APTES film, and the transfer of the measurement from a benchtop spectrometer to a portable unit-rather than a validated diagnostic assay. FAU - Cinar, Kamil AU - Cinar K AUID- ORCID: 0000-0002-1192-6947 AD - Faculty of Science, Department of Physics, Gebze Technical University, 41400 Kocaeli, Turkiye. FAU - Mayack, Christopher AU - Mayack C AD - Department of Biology, College of Science and Health, William Paterson University, Wayne, NJ 07470, USA. FAU - Eraslan-Gultekin, Hulya AU - Eraslan-Gultekin H AUID- ORCID: 0000-0002-4257-3419 AD - Department of Control and Automation Engineering, Faculty of Electrical and Electronic Engineering, Istanbul Technical University, 34469 Istanbul, Turkiye. AD - Department of Electrical and Electronic Engineering, Erzincan Binali Yildirim University, 24002 Erzincan, Turkiye. FAU - Ergenc, Ali Fuat AU - Ergenc AF AD - Department of Control and Automation Engineering, Faculty of Electrical and Electronic Engineering, Istanbul Technical University, 34469 Istanbul, Turkiye. FAU - Kefeli, Melike AU - Kefeli M AUID- ORCID: 0009-0008-9586-0666 AD - Department of Neuroscience, Aziz Sancar Institute of Experimental Medicine, Istanbul University, 34093 Istanbul, Turkiye. FAU - Yurttas, Asiye Gok AU - Yurttas AG AUID- ORCID: 0000-0002-6424-7411 AD - Department of Medical Biochemistry, Faculty of Medicine, Istanbul Atlas University, 34408 Istanbul, Turkiye. LA - eng PT - Journal Article DEP - 20260925 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701862 OTO - NOTNLM OT - APTES functionalization OT - Nosema spp. OT - honeybee health OT - impedance spectroscopy OT - impedimetric transducer COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/09/25 CRDT- 2026/10/27 10:32 PHST- 2026/08/24 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/22 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/09/25 00:00 [pmc-release] AID - bioengineering13101120 [pii] AID - bioengineering-13-01120 [pii] AID - 10.3390/bioengineering13101120 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Sep 25;13(10):1120. doi: 10.3390/bioengineering13101120. PMID- 42938973 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Sep 24 TI - Machine Learning-Based Multiclass Classification of Hb E Carrier Status and Thalassemia-Related Hematological Profiles Using Routine Complete Blood Count Parameters. LID - 10.3390/bioengineering13101118 [doi] LID - 1118 AB - Routine complete blood count (CBC) measurements are widely available and may support the initial assessment of thalassemia-related hematological conditions. However, overlapping hematological profiles and class imbalance can complicate differentiation among groups. This study evaluated seven supervised machine learning classifiers for multiclass classification of normal hematological profiles, suspected alpha-thalassemia profiles, Hb E carrier-related profiles, and other thalassemia-related/hemoglobinopathic profiles using a publicly available CBC dataset. An unresampled baseline was compared with four synthetic oversampling strategies: SMOTE, Borderline-SMOTE, SVM-SMOTE, and KMeans-SMOTE. Model development and hyperparameter optimization were performed within five-fold stratified cross-validation, with model selection based on cross-validated Matthews correlation coefficient (MCC). The untouched internal hold-out test set was reserved for final evaluation. CatBoost without resampling yielded the highest cross-validated MCC (0.6378). On the internal hold-out test set, it achieved an accuracy of 0.7950, an MCC of 0.6537, Cohen's kappa of 0.6499, and micro- and macro-average ROC-AUC values of 0.9554 and 0.9227, respectively. However, class-specific sensitivity was heterogeneous, ranging from 0.4028 for suspected alpha-thalassemia to 0.9767 for the normal hematological profile. Synthetic oversampling did not consistently increase overall CV MCC, although sensitivity improved for some minority classes. These findings characterize the performance and limitations of CBC-based multiclass classification and indicate the need for further external and clinically oriented validation before screening or referral applications can be considered. FAU - Basancelebi, Mert AU - Basancelebi M AUID- ORCID: 0009-0003-4611-3326 AD - Department of Electrical and Electronics Engineering, Zonguldak Bulent Ecevit University, 67100 Zonguldak, Turkiye. FAU - Yapici, Irem Senyer AU - Yapici IS AUID- ORCID: 0000-0003-0655-340X AD - Department of Computer Engineering, Zonguldak Bulent Ecevit University, 67100 Zonguldak, Turkiye. FAU - Uzun Arslan, Rukiye AU - Uzun Arslan R AUID- ORCID: 0000-0002-2082-8695 AD - Department of Electrical and Electronics Engineering, Zonguldak Bulent Ecevit University, 67100 Zonguldak, Turkiye. LA - eng PT - Journal Article DEP - 20260924 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701359 OTO - NOTNLM OT - CatBoost OT - Hb E carrier status OT - class imbalance OT - complete blood count OT - hereditary hemoglobin disorders OT - machine learning OT - thalassemia COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/09/24 CRDT- 2026/10/27 10:32 PHST- 2026/08/29 00:00 [received] PHST- 2026/09/18 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - bioengineering13101118 [pii] AID - bioengineering-13-01118 [pii] AID - 10.3390/bioengineering13101118 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Sep 24;13(10):1118. doi: 10.3390/bioengineering13101118. PMID- 42938972 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Sep 24 TI - From Mechanism to Manufacture: A Narrative Review of Insole Interventions Across Foot Deformity, Limb-Length Discrepancy, and Paediatric Orthopaedic Disease. LID - 10.3390/bioengineering13101117 [doi] LID - 1117 AB - Insoles are among the most widely prescribed devices in orthopaedic practice, spanning conditions as mechanically dissimilar as paediatric flexible flatfoot, painful pes cavus, diabetic neuropathy, inflammatory arthritis, medial knee osteoarthritis, limb-length discrepancy, and neuromuscular gait disorders. Despite this breadth, the field is characterised by a persistent and instructive paradox: the biomechanical effects of insoles are reproducible and quantifiable, whereas their symptomatic effects are inconsistent and frequently indistinguishable from sham. This narrative review, drawing on a bounded, non-systematic evidence base of 55 sources across ten conditions, argues that this paradox is at least partly an engineering and measurement problem rather than a purely clinical one. We develop a mechanistic taxonomy in which every insole acts through some combination of four pathways-plantar pressure redistribution, external joint moment modulation, soft-tissue strain reduction, and sensorimotor modulation-and map the controllable design parameters (arch support height, medial posting angle, heel cup depth, thickness, material stiffness, and lattice architecture) onto each pathway using sensitivity data from a small number of heterogeneous finite element and Taguchi-based optimisation studies, several of which are single-model or single-patient analyses with limited experimental validation. We then review the manufacturing pathway from morphological capture through computer-aided design, additive or subtractive fabrication, and in-shoe verification, and summarise the condition-specific clinical evidence across the ten indications. Three hypothesis-generating findings emerge, each requiring prospective confirmation before being treated as established. First, custom devices consistently modify mechanics more than prefabricated devices, yet consistently fail to outperform them on symptom endpoints, indicating that the mechanism-outcome link is mediated by unmeasured variables. Second, the biomechanical response is heterogeneous in several conditions-for example, roughly one-third of patients with medial knee osteoarthritis show an increase rather than a decrease in the knee adduction moment with a lateral wedge-raising the possibility that population-averaged trials may obscure responder-specific effects, although the one trial that acted on this logic by individually tailoring the wedge angle found no clinical benefit. Third, a secondary analysis of a single randomised trial in painful pes cavus found that the redistribution, rather than magnitude, of plantar pressure predicted pain relief; if replicated, this would suggest that peak plantar pressure is an incomplete outcome measure in that specific pain-related context, a claim we do not extend to conditions such as diabetic offloading, where peak local pressure remains a mechanistically appropriate proxy for a different, site-specific outcome. We propose a design-decision framework, presented explicitly as a falsifiable hypothesis rather than a validated protocol, that selects design parameters by target mechanism rather than by diagnostic label, and argue that closed-loop prescription-instrumented insoles providing the continuous verification of both mechanical target attainment and wear adherence-is a promising but as-yet-unproven direction for the field. FAU - Jeon, Se Yeong AU - Jeon SY AUID- ORCID: 0009-0008-7279-4862 AD - Science and Technology Department, Ulsan National Institute of Science and Technology, Ulsan 44919, Republic of Korea. FAU - Jung, Chul Hee AU - Jung CH AUID- ORCID: 0000-0002-1965-7315 AD - Department of Orthopaedic Surgery, Aerospace Medical Center, Republic of Korea Air Force, Cheongju 28187, Republic of Korea. FAU - Choi, Seok Yeon AU - Choi SY AUID- ORCID: 0000-0003-4720-4580 AD - Department of Rehabilitation Medicine, Aerospace Medical Center, Republic of Korea Air Force, Cheongju 28187, Republic of Korea. FAU - Lee, Dong Ha AU - Lee DH AUID- ORCID: 0000-0001-7826-2148 AD - Department of Orthopaedic Surgery, Aerospace Medical Center, Republic of Korea Air Force, Cheongju 28187, Republic of Korea. AD - Department of Orthopaedic Surgery, Seoul National University Hospital, Seoul 03080, Republic of Korea. LA - eng PT - Journal Article PT - Review DEP - 20260924 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701568 OTO - NOTNLM OT - additive manufacturing OT - finite element analysis OT - flatfoot OT - foot orthosis OT - insole OT - limb-length discrepancy OT - paediatric orthopaedics OT - pes cavus OT - plantar pressure OT - smart insole COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/09/24 CRDT- 2026/10/27 10:32 PHST- 2026/08/14 00:00 [received] PHST- 2026/09/12 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - bioengineering13101117 [pii] AID - bioengineering-13-01117 [pii] AID - 10.3390/bioengineering13101117 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Sep 24;13(10):1117. doi: 10.3390/bioengineering13101117. PMID- 42938968 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Sep 24 TI - High-Resolution Ex Vivo Confocal Microscopy to Distinguish Cutaneous T-Cell Lymphoma from Inflammatory Mimickers. LID - 10.3390/bioengineering13101114 [doi] LID - 1114 AB - Cutaneous T-cell lymphoma (CTCL) often clinically resembles inflammatory dermatoses such as eczema, making accurate and timely diagnosis challenging. Since early differentiation is critical for appropriate treatment and prognosis, fast and reliable diagnostic tools are needed. Ex vivo confocal laser scanning microscopy (EVCM) has emerged as a promising technique for rapid, high-resolution tissue assessment. As CTCL is difficult to identify based on clinical presentation alone and is frequently misdiagnosed as eczema, this study assessed the diagnostic accuracy and clinical utility of EVCM in differentiating CTCL from eczema, as well as interobserver agreement among examiners with varying experience. Fifty specimens (25 eczema, 25 CTCL) were imaged using EVCM (Vivascope 2500 M-G4; Vivascope GmbH, Munich, Germany). Three independent, blinded dermatologists with different experience levels classified lesions based solely on digital H&E-like images. Sensitivity, specificity, and predictive values were calculated. EVCM showed high diagnostic accuracy, with a mean concordance rate of 82.67%. The dermatohistopathologist achieved the highest accuracy (84%), followed by two trained dermatologists (82% each). Sensitivity ranged from 0.80 to 0.88 and specificity from 0.76 to 0.84, with comparable predictive values and almost perfect interobserver agreement (Fleiss' kappa = 0.89). Misclassifications mainly involved the same diagnostically challenging cases across both entities. EVCM demonstrated high diagnostic accuracy across examiners of varying experience, supporting its potential as a rapid bedside tool for distinguishing CTCL from eczema. While not replacing histopathology as the gold standard, it may meaningfully complement routine diagnostics. Integration of immunohistochemistry or artificial intelligence could further enhance diagnostic precision. FAU - Hartmann, Daniela AU - Hartmann D AUID- ORCID: 0000-0002-1002-8133 AD - Department of Dermatology and Allergy, LMU Medizin, LMU University Hospital, Ludwig-Maximilians-Universitat Munich, 80337 Munich, Germany. AD - Department of Dermatology and Allergy, Munich Municipal Hospital, 80337 Munich, Germany. FAU - Braun, Aimee AU - Braun A AD - Department of Dermatology and Allergy, LMU Medizin, LMU University Hospital, Ludwig-Maximilians-Universitat Munich, 80337 Munich, Germany. FAU - Kendziora, Benjamin AU - Kendziora B AUID- ORCID: 0000-0002-5234-5380 AD - Department of Dermatology and Allergy, LMU Medizin, LMU University Hospital, Ludwig-Maximilians-Universitat Munich, 80337 Munich, Germany. AD - Department of Dermatology and Allergy, Munich Municipal Hospital, 80337 Munich, Germany. FAU - Wex, Katharina AU - Wex K AD - Department of Dermatology and Allergy, LMU Medizin, LMU University Hospital, Ludwig-Maximilians-Universitat Munich, 80337 Munich, Germany. FAU - Pabst, Paulina AU - Pabst P AD - Department of Dermatology and Allergy, LMU Medizin, LMU University Hospital, Ludwig-Maximilians-Universitat Munich, 80337 Munich, Germany. FAU - Ohlmann, Andreas AU - Ohlmann A AUID- ORCID: 0000-0002-7101-9361 AD - Department of Ophthalmology, LMU University Hospital, LMU Munich, 80336 Munich, Germany. FAU - Sattler, Elke C AU - Sattler EC AD - Department of Dermatology and Allergy, LMU Medizin, LMU University Hospital, Ludwig-Maximilians-Universitat Munich, 80337 Munich, Germany. FAU - Deussing, Maximilian AU - Deussing M AUID- ORCID: 0000-0003-4935-9877 AD - Department of Dermatology and Allergy, LMU Medizin, LMU University Hospital, Ludwig-Maximilians-Universitat Munich, 80337 Munich, Germany. AD - Department of Dermatology and Allergy, Munich Municipal Hospital, 80337 Munich, Germany. LA - eng PT - Journal Article DEP - 20260924 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701392 OTO - NOTNLM OT - bedside histology OT - dermatology OT - digital pathology OT - non-invasive imaging OT - oncology COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/09/24 CRDT- 2026/10/27 10:32 PHST- 2026/09/05 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - bioengineering13101114 [pii] AID - bioengineering-13-01114 [pii] AID - 10.3390/bioengineering13101114 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Sep 24;13(10):1114. doi: 10.3390/bioengineering13101114. PMID- 42938966 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Sep 24 TI - DCD-VMUNet: A Dynamic Multi-Scale and Geometry-Aware Network for Coronary Vessel and Stenosis Segmentation in X-Ray Angiography. LID - 10.3390/bioengineering13101111 [doi] LID - 1111 AB - Accurate segmentation of coronary vessels and stenotic regions in X-ray coronary angiography remains challenging because of low vessel-to-background contrast, vessel overlap, thin branches, and the small, ambiguous appearance of stenotic lesions. In this study, we propose DCD-VMUNet, a structure-aware framework for automated coronary vessel and stenosis segmentation. Built on VM-UNet, the model incorporates a dynamic multi-scale kernel module, a centerline head, and a direction head. The dynamic module adaptively integrates fine boundary details with broader contextual cues, while the auxiliary heads introduce geometric supervision to enhance structural continuity and orientation awareness. DCD-VMUNet was independently trained and evaluated for binary vessel and stenosis segmentation on the ARCADE dataset. It was compared with YOLOv11-X, DeepLab v3+, UNet, UNet++, TransUNet, MALUNet, and VM-UNet using pixel-wise metrics and, for vessel segmentation, the centerline-aware clDice metric. For vessel segmentation, DCD-VMUNet achieved the best performance, with IoU, precision, recall, and F1-score of 0.6838, 0.8418, 0.7846, and 0.8122. For stenosis segmentation, it also ranked first across all metrics, reaching 0.4042, 0.5826, 0.5688, and 0.5757, respectively. Ablation studies confirmed the complementary effects of multi-scale feature extraction and geometric supervision. Additional evaluation on the independent ICA dataset further supported the vessel segmentation performance of DCD-VMUNet beyond the ARCADE benchmark. These results indicate that DCD-VMUNet improves coronary vessel delineation and pixel-level stenosis localization, supporting automated coronary angiographic assessment. FAU - Quan, Youzhuo AU - Quan Y AUID- ORCID: 0009-0000-6056-1414 AD - Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. AD - Hubei Key Laboratory of Biological Targeted Therapy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. AD - Hubei Engineering Research Center for Immunological Diagnosis and Therapy of Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. FAU - Yue, Huaizhi AU - Yue H AD - School of Information Engineering, Chang'an University, Xi'an 710064, China. FAU - Ma, Xiangyang AU - Ma X AD - Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. AD - Hubei Key Laboratory of Biological Targeted Therapy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. AD - Hubei Engineering Research Center for Immunological Diagnosis and Therapy of Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. FAU - Liu, Kun AU - Liu K AD - Department of Cardiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. AD - Hubei Key Laboratory of Biological Targeted Therapy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. AD - Hubei Engineering Research Center for Immunological Diagnosis and Therapy of Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. LA - eng PT - Journal Article DEP - 20260924 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701670 OTO - NOTNLM OT - X-ray coronary angiography OT - coronary stenosis localization OT - coronary vessel segmentation OT - deep learning OT - medical image segmentation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/09/24 CRDT- 2026/10/27 10:32 PHST- 2026/09/05 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/09/24 00:00 [pmc-release] AID - bioengineering13101111 [pii] AID - bioengineering-13-01111 [pii] AID - 10.3390/bioengineering13101111 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Sep 24;13(10):1111. doi: 10.3390/bioengineering13101111. PMID- 42938957 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Sep 23 TI - From Ionic Agitation to Precision Neuroablation: A Bioengineering Perspective on the Mechanisms, Modalities, and Clinical Applications of Radiofrequency Technology in Pain Management. LID - 10.3390/bioengineering13101102 [doi] LID - 1102 AB - Radiofrequency ablation (RFA) is a foundational interventional technology in pain management. Despite the diversity of available systems, conventional thermal, internally cooled, bipolar, and pulsed radiofrequency (PRF) all share one biophysical mechanism: high-frequency alternating current (AC) drives tissue ions to oscillate, generating frictional heat within the tissue surrounding the electrode. This localized heating produces predictable patterns of coagulative necrosis shaped by tissue electrical conductivity, electrode geometry, power delivery, and perfusion-related heat dissipation. Classical and contemporary bioheat models, including the Pennes bioheat equation, describe how these variables determine lesion size and morphology. Advances in device engineering have reshaped lesion characteristics through internally cooled probes, bipolar strip-lesioning systems, and pulsed platforms that modulate neural activity while preserving structural integrity. These innovations broaden therapeutic options by enabling targeting of anatomically variable structures, such as the sacral lateral branches, and intraosseous targets, including the basivertebral nerve (BVN). Emerging technologies-high-voltage PRF, hybrid pulsed-thermal systems, and electroporation-based pulsed-field ablation-reflect a growing emphasis on tissue selectivity, energy efficiency, and safety. This narrative review traces the historical development of radiofrequency neurotomy, examines the biophysical principles governing lesion formation (including the Pennes bioheat equation and the roles of current density, impedance, and perfusion), and details the engineering distinctions among modalities. Clinical applications across spinal, joint, and peripheral nerve targets are presented to illustrate how these physical and engineering differences translate into procedural decision-making, and emerging modalities such as high-intensity focused ultrasound (HIFU) and pulsed-field ablation are discussed in the context of the biophysical principles they extend or depart from. FAU - Poole, Eric E AU - Poole EE AD - Department of Physical Medicine and Rehabilitation, Charles R. Drew University of Medicine and Science, 1731 E. 120th St., Los Angeles, CA 90059, USA. FAU - Odonkor, Charles A AU - Odonkor CA AUID- ORCID: 0000-0002-8280-4015 AD - Department of Orthopedics and Rehabilitation, Division of Physiatry, Interventional Pain Medicine, Yale New Haven Hospital, 20 York Street, New Haven, CT 06510, USA. AD - Department of Therapeutic Radiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA. FAU - Nguyen, Eric T AU - Nguyen ET AUID- ORCID: 0009-0009-4923-248X AD - Department of Physical Medicine and Rehabilitation, Charles R. Drew University of Medicine and Science, 1731 E. 120th St., Los Angeles, CA 90059, USA. FAU - Lee, David W AU - Lee DW AD - Department of Physical Medicine and Rehabilitation, University of California, Irvine, 101 The City Drive South, Orange, CA 92868, USA. FAU - Dodurgali, Mustafa R AU - Dodurgali MR AUID- ORCID: 0009-0000-6468-8009 AD - Department of Orthopedics and Rehabilitation, Division of Physiatry, Interventional Pain Medicine, Yale New Haven Hospital, 20 York Street, New Haven, CT 06510, USA. FAU - Draeger, Emily AU - Draeger E AUID- ORCID: 0000-0003-4664-2642 AD - Department of Therapeutic Radiology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA. FAU - Abd-Elsayed, Alaa AU - Abd-Elsayed A AUID- ORCID: 0000-0002-9890-7860 AD - Department of Anesthesiology, University of Wisconsin, Madison, 600 Highland Avenue, Madison, WI 53792, USA. LA - eng PT - Journal Article PT - Review DEP - 20260923 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701832 OTO - NOTNLM OT - basivertebral nerve OT - bioheat modeling OT - electroporation OT - neuromodulation OT - pain interventions OT - peripheral nerves OT - radiofrequency ablation OT - spine pain COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/09/23 CRDT- 2026/10/27 10:32 PHST- 2026/07/14 00:00 [received] PHST- 2026/09/11 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - bioengineering13101102 [pii] AID - bioengineering-13-01102 [pii] AID - 10.3390/bioengineering13101102 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Sep 23;13(10):1102. doi: 10.3390/bioengineering13101102. PMID- 42938954 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2306-5354 (Print) IS - 2306-5354 (Electronic) IS - 2306-5354 (Linking) VI - 13 IP - 10 DP - 2026 Sep 23 TI - Classifier-Derived Guidance for Diffusion-Based Medical Dataset Distillation. LID - 10.3390/bioengineering13101100 [doi] LID - 1100 AB - Medical image analysis often relies on large annotated datasets, whose collection, storage, and sharing are costly and constrained by privacy concerns. Dataset distillation offers a potential solution by constructing compact synthetic datasets that retain task-relevant information from the original data. However, generative distillation methods may not adequately preserve class-specific information in medical images, where clinically distinct classes often exhibit similar visual characteristics. To address this issue, we propose a classifier-derived guidance framework for diffusion-based medical dataset distillation. The framework uses predictions from a pretrained noise-conditioned classifier to guide diffusion sampling through three objectives. Classifier guidance (CG) promotes class alignment, whereas entropy and margin guidance encourage boundary-oriented sampling. We analyze guidance-strength sensitivity on three medical imaging datasets across images-per-class (IPC) budgets and evaluate validation-selected COVID-19 Radiography configurations on the test subset using three downstream architectures. Classifier diagnostics distinguish prediction-conditioning agreement from downstream utility, while learning curves illustrate differences in validation behavior despite low training loss. These findings characterize the configuration-dependent utility of classifier-derived guidance for compact synthetic medical datasets. FAU - Du, Shuailin AU - Du S AUID- ORCID: 0009-0005-7996-7699 AD - Graduate School of Information Science and Technology, Hokkaido University, N-14, W-9, Kita-ku, Sapporo 060-0814, Hokkaido, Japan. FAU - Li, Guang AU - Li G AUID- ORCID: 0000-0003-2898-2504 AD - Education and Research Center for Mathematical and Data Science, Hokkaido University, N-13, W-10, Kita-ku, Sapporo 060-0813, Hokkaido, Japan. FAU - Togo, Ren AU - Togo R AUID- ORCID: 0000-0002-4474-3995 AD - Faculty of Information Science and Technology, Hokkaido University, N-14, W-9, Kita-ku, Sapporo 060-0814, Hokkaido, Japan. FAU - Ogawa, Takahiro AU - Ogawa T AUID- ORCID: 0000-0001-5332-8112 AD - Faculty of Information Science and Technology, Hokkaido University, N-14, W-9, Kita-ku, Sapporo 060-0814, Hokkaido, Japan. FAU - Haseyama, Miki AU - Haseyama M AUID- ORCID: 0000-0003-1496-1761 AD - Faculty of Information Science and Technology, Hokkaido University, N-14, W-9, Kita-ku, Sapporo 060-0814, Hokkaido, Japan. LA - eng GR - JP24K02942/Japan Society for the Promotion of Science/ GR - JP25K21218/Japan Society for the Promotion of Science/ GR - JP266f0137006/Japan Agency for Medical Research and Development/ PT - Journal Article DEP - 20260923 PL - Switzerland TA - Bioengineering (Basel) JT - Bioengineering (Basel, Switzerland) JID - 101676056 PMC - PMC13701449 OTO - NOTNLM OT - classifier guidance OT - dataset distillation OT - diffusion models OT - medical imaging OT - synthetic data COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/09/23 CRDT- 2026/10/27 10:32 PHST- 2026/08/28 00:00 [received] PHST- 2026/09/19 00:00 [revised] PHST- 2026/09/21 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/09/23 00:00 [pmc-release] AID - bioengineering13101100 [pii] AID - bioengineering-13-01100 [pii] AID - 10.3390/bioengineering13101100 [doi] PST - epublish SO - Bioengineering (Basel). 2026 Sep 23;13(10):1100. doi: 10.3390/bioengineering13101100. PMID- 42938944 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 21 TI - Lymphatic Microvascular Density, Lymphatic Invasion and Endothelial Cell Proliferation as Prognostic Biomarkers in Cutaneous Melanoma: A Systematic Review and Evidence Map. LID - 10.3390/cancers18203412 [doi] LID - 3412 AB - Background/Objectives: Cutaneous melanoma spreads preferentially through the lymphatic system, and sentinel lymph node status is among the strongest prognostic factors. Whether tumour angiogenesis and lymphangiogenesis add prognostic information beyond conventional histopathology has been examined in many immunohistochemical studies, with heterogeneous results. We conducted a systematic review, reported according to PRISMA 2020 and not registered, of blood microvascular density (MVD), lymphatic vessel density (LVD) in the intratumoral and peritumoral compartments, proliferating (Ki-67-positive) lymphatic vessels and lymphatic invasion (LI) as prognostic markers in primary cutaneous melanoma. Methods: PubMed/MEDLINE was searched to 10 September 2026, supplemented by a Web of Science export and by citation tracking of the reference meta-analysis by Pastushenko et al. (2014). Twenty-one cutaneous study units (25 reports) and four ocular studies providing indirect evidence were included, and risk of bias was rated with the QUIPS tool. Results: In the reference meta-analysis, peritumoral LVD and LI, but not MVD or intratumoral LVD, were associated with metastasis. The newer cohorts confirmed this pattern and provided adjusted effect sizes: peritumoral LVD predicted disease-free (HR 2.14) and melanoma-specific survival (HR of 2.74; 95% CI 1.31 to 5.73), immunohistochemically detected LI predicted sentinel node metastasis (33% vs. 11% positive nodes) and overall survival, and a Ki-67-positive lymphatic endothelium predicted melanoma-specific mortality (adjusted HR of 2.03; 95% CI 1.33 to 3.10) in 264 patients. Blood MVD and blood endothelial proliferation were not prognostic. Heterogeneity of markers, counting methods and effect measures precluded re-pooling. Conclusions: Peritumoral LVD, LI and proliferating lymphatics are promising adjunct biomarkers for selecting patients for sentinel node biopsy, adjuvant therapy and closer follow-up, pending standardised, digital-pathology-based quantification. FAU - Dumitrascu, Victor Cristian AU - Dumitrascu VC AD - Doctoral School, Faculty of Medicine, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania. FAU - Ceausu, Amalia Raluca AU - Ceausu AR AUID- ORCID: 0000-0002-0067-8247 AD - Department of Microscopic Morphology, Histology, Angiogenesis Research Center, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania. FAU - Mituletu, Mihai AU - Mituletu M AD - ANAPATMOL Research Center, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania. AD - Department of Microscopic Morphology, Discipline of Cellular and Molecular Biology, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania. FAU - Muntean, Calin AU - Muntean C AUID- ORCID: 0000-0002-0497-0405 AD - Department of Informatics and Medical Biostatistics, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania. FAU - Popescu, Roxana AU - Popescu R AUID- ORCID: 0000-0002-9387-1141 AD - ANAPATMOL Research Center, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania. AD - Department of Microscopic Morphology, Discipline of Cellular and Molecular Biology, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania. FAU - Dumitrescu, Gabi AU - Dumitrescu G AUID- ORCID: 0000-0001-9045-6631 AD - ANAPATMOL Research Center, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania. AD - Faculty of Bioengineering of Animal Resources, University of Life Sciences "King Mihai I" from Timisoara, 119 Calea Aradului, 300645 Timisoara, Romania. FAU - Gaje, Pusa Nela AU - Gaje PN AD - Department of Microscopic Morphology, Histology, Angiogenesis Research Center, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania. FAU - Raica, Marius AU - Raica M AD - Department of Microscopic Morphology, Histology, Angiogenesis Research Center, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania. FAU - Vlad, Cristian Sebastian AU - Vlad CS AD - Department of Biochemistry and Pharmacology, Faculty of Medicine, "Victor Babes" University of Medicine and Pharmacy, 2 Eftimie Murgu Square, 300041 Timisoara, Romania. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702970 OTO - NOTNLM OT - D2-40 OT - Ki-67 OT - cutaneous melanoma OT - evidence map OT - lymphangiogenesis OT - lymphatic invasion OT - lymphatic vessel density OT - microvascular density OT - podoplanin OT - sentinel lymph node OT - systematic review COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/21 CRDT- 2026/10/27 10:32 PHST- 2026/09/24 00:00 [received] PHST- 2026/10/17 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - cancers18203412 [pii] AID - cancers-18-03412 [pii] AID - 10.3390/cancers18203412 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 21;18(20):3412. doi: 10.3390/cancers18203412. PMID- 42938943 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 21 TI - Targeting Polyploid Giant Cancer Cells (PGCCs) to Prevent Cancer Recurrence: Opportunities for Nanomedicine. LID - 10.3390/cancers18203411 [doi] LID - 3411 AB - Background/Hypothesis: Therapeutic resistance and recurrence remain major barriers to durable cancer control because conventional therapies may eliminate proliferating tumor cells while allowing dormant, stem-like, senescence-like, or polyploid populations to persist. This review examines polyploid giant cancer cells (PGCCs) as therapy-tolerant states and evaluates whether their adaptive survival mechanisms create vulnerabilities that could be targeted through ferroptosis-oriented nanomedicine. Methods: We investigated recent evidence (PubMed) linking PGCC persistence with redox buffering, glutathione/GPX4 activity, iron regulation, lipid remodeling, autophagy, mitochondrial adaptation, and metabolic plasticity, in addition to nanomedicine potential to target these pathways. Results and Findings: Emerging studies provide direct proof-of-concept that PGCCs can exhibit ferroptosis susceptibility, while nanomedicine offers potential strategies to improve therapeutic localization and coordinate iron delivery, reactive oxygen species amplification, glutathione depletion, GPX4 inhibition, and combination therapy. However, PGCC-selective nanoparticle efficacy remains largely untested, and important barriers include heterogeneous tumor penetration, off-target toxicity, model limitations, and biomarker uncertainty. Conclusions and Perspective: The specific contribution of this review is to integrate PGCC biology, therapy-tolerant cancer states, ferroptosis, and nanomedicine into a unified framework for targeting residual disease. This perspective is timely as direct evidence of PGCC ferroptosis sensitivity and new characterization of treatment-resistant PGCC states are emerging. By identifying the mechanistic overlap among these fields, this review highlights testable opportunities for eliminating persistent cancer cells that may otherwise contribute to recurrence. FAU - Norton, Jillian AU - Norton J AD - School of Pharmacy, Pacific University, Hillsboro, OR 97123, USA. FAU - Naguib, Youssef W AU - Naguib YW AD - School of Pharmacy, Pacific University, Hillsboro, OR 97123, USA. LA - eng GR - Pacific University/ GR - Faculty Development Seed Grant./Pacific University/ PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702786 OTO - NOTNLM OT - PGCC OT - cancer recurrence OT - cancer resistance OT - ferroptosis OT - nanomedicine OT - polyploid giant cancer cells OT - stress adaptation OT - therapy tolerance COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/21 CRDT- 2026/10/27 10:32 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - cancers18203411 [pii] AID - cancers-18-03411 [pii] AID - 10.3390/cancers18203411 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 21;18(20):3411. doi: 10.3390/cancers18203411. PMID- 42938942 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 21 TI - Context-Dependent Spatial and Functional Roles of CD44 Across Pancreatic Ductal Adenocarcinoma States. LID - 10.3390/cancers18203410 [doi] LID - 3410 AB - Background/Objectives: Pancreatic ductal adenocarcinoma (PDAC) comprises distinct cellular states characterized by different differentiation programs and therapeutic responses. How a cell-surface receptor adapts its function across these states remains poorly understood. Here, we investigated whether the spatial organization and functional output of CD44 vary across PDAC cellular states. Methods: CD44 expression and spatial distribution were examined in human PDAC tissues and PDAC cell models representing different differentiation states. The functional roles of CD44 were investigated using genetic depletion, transcriptomic analysis, migration and invasion assays, and xenograft and orthotopic models. Hyaluronan (HA) responsiveness and downstream signaling were assessed following exogenous HA stimulation, and the therapeutic effects of HA synthesis inhibition with 4-methylumbelliferone (4-MU) were evaluated alone or in combination with gemcitabine. Results: In differentiated PDAC tissues and classical PDAC models, CD44 was predominantly localized to the basolateral membrane and spatially segregated from E-cadherin at cell-cell junctions, consistent with a role in epithelial organization and cell-matrix interactions. In contrast, poorly differentiated PDAC tissues and basal-like models exhibited increased CD44 abundance, E-cadherin loss, and redistribution of CD44 along the cell periphery. Genetic depletion of CD44 preferentially impaired malignant phenotypes in basal-like models. Mechanistically, basal-like cells exhibited enhanced CD44-dependent responsiveness to extracellular HA, accompanied by activation of EMT-associated signaling and destabilization of E-cadherin. Inhibition of HA synthesis with 4-MU preferentially suppressed basal-like PDAC models and enhanced gemcitabine sensitivity, with these effects further supported in orthotopic models. Conclusions: These findings suggest that the functional output of CD44 in PDAC is influenced not only by its expression level but also by cellular context and spatial organization. The context-dependent responsiveness of the HA-CD44 axis may contribute to functional heterogeneity among PDAC states and represents a potential therapeutic vulnerability of basal-like PDAC. FAU - Fu, Bin AU - Fu B AD - State Key Laboratory of Natural Medicines, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, China. FAU - Tan, Yingying AU - Tan Y AD - State Key Laboratory of Natural Medicines, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, China. FAU - Chen, Yaxin AU - Chen Y AD - State Key Laboratory of Natural Medicines, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, China. FAU - Su, Yongren AU - Su Y AD - State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Nankai University, Tianjin 300071, China. FAU - Cao, Mengran AU - Cao M AD - State Key Laboratory of Natural Medicines, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, China. FAU - Tan, Dashan AU - Tan D AD - State Key Laboratory of Natural Medicines, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, China. FAU - Zhang, Meng AU - Zhang M AD - State Key Laboratory of Natural Medicines, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, China. FAU - Chen, Xuewei AU - Chen X AD - State Key Laboratory of Natural Medicines, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, China. FAU - Hu, Rong AU - Hu R AD - State Key Laboratory of Natural Medicines, School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, China. LA - eng GR - 81872337/National Natural Science Foundation of China/ GR - 82073185/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261021 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702738 OTO - NOTNLM OT - CD44 OT - Pancreatic Ductal Adenocarcinoma (PDAC) OT - cellular plasticity OT - hyaluronan OT - spatial organization COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/21 CRDT- 2026/10/27 10:32 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - cancers18203410 [pii] AID - cancers-18-03410 [pii] AID - 10.3390/cancers18203410 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 21;18(20):3410. doi: 10.3390/cancers18203410. PMID- 42938941 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 21 TI - Implant-Based Versus Autologous Reconstruction After Salvage Mastectomy for Ipsilateral Breast Tumor Recurrence in the Previously Irradiated Breast: A Consecutive Institutional Series and a Proposed Autologous-First Algorithm. LID - 10.3390/cancers18203409 [doi] LID - 3409 AB - Background: In postmastectomy radiotherapy, radiation reaches an already-reconstructed breast, and implant-based reconstruction remains feasible in selected patients. Far less studied is reconstruction within a previously irradiated field: after breast-conserving therapy, ipsilateral breast tumor recurrence (IBTR), and salvage mastectomy. We compared implant-based and autologous reconstruction in this setting and propose a hypothesis-generating treatment algorithm. Methods: From 2219 consecutive breast reconstructions (2012-2026), we cross-matched implant-removal, radiotherapy, and local-recurrence registries to identify all immediate total reconstructions of a previously irradiated breast after salvage mastectomy for IBTR (n = 13; 8 implant-based [all stand-alone, without vascularized flap coverage], 5 autologous [all latissimus dorsi-based]). The primary endpoint was reconstruction failure, defined in a modality-neutral manner as definitive loss or abandonment of the reconstruction; implant-based reconstructions of breasts not previously irradiated at the time of reconstruction (n = 689) served as a descriptive institutional reference cohort. Results: Seven of eight implants (87.5%) were removed at 3 to 51 months; the single retained implant has a persistent wound; none achieved complication-free success. Failure modes comprised early envelope necrosis, periprosthetic infection, and late attritional failure; chronic retained-capsule seroma followed removal without capsulectomy. All five autologous reconstructions healed without failure or unplanned reoperation (reconstruction failure, 7/8 [87.5%; 95% CI, 47.3-99.7%] vs. 0/5 [0%; 95% CI, 0-52.2%], p = 0.005; log-rank p = 0.039; median follow-up, 32.0 vs. 10.9 months; failure or major complication, 8/8 vs. 0/5, p = 0.0008). The implant-removal rate also exceeded that of the institutional reference cohort (87.5% vs. 11.2%; unadjusted, descriptive comparison). Conclusions: In this small consecutive series, stand-alone implant-based reconstruction of the previously irradiated breast was associated with a very high failure rate, whereas reconstruction importing well-vascularized autologous tissue healed in every case, a pattern biologically consistent with chronic radiation injury. These findings constitute a strong cautionary signal rather than definitive evidence. Our findings support an autologous-first approach after salvage mastectomy of the irradiated breast; stand-alone implant-based reconstruction should be offered only to carefully selected, fully counseled patients, and the proposed algorithm requires external validation. FAU - Park, Jongmoo AU - Park J AD - Department of Radiation Oncology, School of Medicine, Kyungpook National University, Daegu 41566, Republic of Korea. FAU - Jang, Woosung AU - Jang W AD - Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University, Daegu 41566, Republic of Korea. FAU - Kang, Myeong Jae AU - Kang MJ AD - Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University, Daegu 41566, Republic of Korea. FAU - Kim, Sung Joon AU - Kim SJ AUID- ORCID: 0000-0002-8963-7467 AD - Department of Radiation Oncology, Kyungpook National University Chilgok Hospital, Daegu 41404, Republic of Korea. FAU - Ko, Byoung-Soo AU - Ko BS AUID- ORCID: 0000-0003-1415-4433 AD - Department of Radiation Oncology, Kyungpook National University Chilgok Hospital, Daegu 41404, Republic of Korea. FAU - Lee, In Hee AU - Lee IH AD - Department of Oncology/Hematology, Kyungpook National University Chilgok Hospital, School of Medicine, Kyungpook National University, Daegu 41566, Republic of Korea. FAU - Moon, Joon Suk AU - Moon JS AUID- ORCID: 0009-0009-2880-6472 AD - Department of Surgery, School of Medicine, Kyungpook National University, Daegu 41566, Republic of Korea. FAU - Kang, Byeongju AU - Kang B AD - Department of Surgery, School of Medicine, Kyungpook National University, Daegu 41566, Republic of Korea. FAU - Lee, Jeeyeon AU - Lee J AUID- ORCID: 0000-0003-1826-1690 AD - Department of Surgery, School of Medicine, Kyungpook National University, Daegu 41566, Republic of Korea. FAU - Park, Ho Yong AU - Park HY AD - Department of Surgery, School of Medicine, Kyungpook National University, Daegu 41566, Republic of Korea. FAU - Ryu, Jeong Yeop AU - Ryu JY AD - Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University, Daegu 41566, Republic of Korea. FAU - Choi, Kang Young AU - Choi KY AD - Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University, Daegu 41566, Republic of Korea. FAU - Yang, Jung Dug AU - Yang JD AD - Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University, Daegu 41566, Republic of Korea. FAU - Chung, Ho Yun AU - Chung HY AUID- ORCID: 0000-0001-7359-3044 AD - Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University, Daegu 41566, Republic of Korea. FAU - Lee, Joon Seok AU - Lee JS AUID- ORCID: 0000-0002-1580-0487 AD - Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University, Daegu 41566, Republic of Korea. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702566 OTO - NOTNLM OT - breast cancer OT - breast implant OT - breast reconstruction OT - ipsilateral breast tumor recurrence OT - irradiated breast OT - latissimus dorsi flap OT - radiotherapy OT - salvage mastectomy OT - treatment algorithm COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/21 CRDT- 2026/10/27 10:32 PHST- 2026/09/19 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - cancers18203409 [pii] AID - cancers-18-03409 [pii] AID - 10.3390/cancers18203409 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 21;18(20):3409. doi: 10.3390/cancers18203409. PMID- 42938937 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 21 TI - Optic Nerve Sheath Meningiomas: A Narrative Review of Clinical Features, Imaging Advances, and Current Management. LID - 10.3390/cancers18203405 [doi] LID - 3405 AB - Optic nerve sheath meningioma (ONSM) is a rare orbital tumor that can pose a significant threat to visual function due to its close relationship with the optic nerve and surrounding vascular structures. Early and accurate diagnosis is essential to preserve vision and guide individualized management. The objective of this narrative review is to provide a comprehensive overview of the current evidence on the clinical presentation, diagnostic evaluation, imaging characteristics, and management of ONSM, with a particular focus on recent advances in imaging techniques and their implications for clinical decision-making. This review synthesizes current knowledge on clinical presentation, diagnostic workup, and evolving treatment strategies for ONSM, with particular emphasis on advanced imaging modalities. Magnetic resonance imaging remains the cornerstone of diagnosis, yet functional imaging, especially somatostatin receptor--based techniques such as 68GaDOTATATE PET/CT have shown potential as a useful adjunct in the diagnostic evaluation of ONSM, especially for assessing tumor characteristics, extent, and treatment planning in selected cases. We discuss the emerging role of DOTATATE imaging in differentiating ONSM from other optic nerve and orbital pathologies, as well as its potential application in monitoring therapeutic response. We also highlight contemporary management approaches, including fractionated radiotherapy and the selective role of surgical intervention, underscoring the importance of multidisciplinary decision-making in optimizing visual outcomes. Emerging molecular insights from meningioma research may inform future therapeutic strategies for ONSM; however, clinical evidence specific to ONSM remains limited. FAU - Sadeghi, Nima AU - Sadeghi N AUID- ORCID: 0009-0001-5751-8463 AD - Department of Ophthalmology, The Orbital Center, Amsterdam University Medical Center, 1081 HV Amsterdam, The Netherlands. FAU - Laban, Kamil AU - Laban K AUID- ORCID: 0000-0002-8817-371X AD - Department of Ophthalmology, The Orbital Center, Amsterdam University Medical Center, 1081 HV Amsterdam, The Netherlands. AD - Department of Ophthalmology, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands. FAU - Saeed, Peerooz AU - Saeed P AD - Department of Ophthalmology, The Orbital Center, Amsterdam University Medical Center, 1081 HV Amsterdam, The Netherlands. LA - eng PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702578 OTO - NOTNLM OT - DOTATATE Scan OT - meningioma OT - optic nerve sheath OT - radiotherapy COIS- The authors declare no conflict of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/21 CRDT- 2026/10/27 10:32 PHST- 2026/09/24 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/17 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - cancers18203405 [pii] AID - cancers-18-03405 [pii] AID - 10.3390/cancers18203405 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 21;18(20):3405. doi: 10.3390/cancers18203405. PMID- 42938933 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 21 TI - Immune Cell Engagers and "Masking Immunotherapy" for Solid Tumors: Focus on Head and Neck Cancers. LID - 10.3390/cancers18203401 [doi] LID - 3401 AB - Head and neck squamous cell carcinomas (HNSCCs) are challenging pathologies to treat with immune checkpoint inhibitors (ICIs), as only a minority of patients achieve durable clinical benefit. The highly immunosuppressive tumor microenvironment (TME), the frequent loss of antigen presentation, the marked spatial heterogeneity, and the anatomical proximity of tumors to critical structures limit the efficacy and safety of current immunotherapeutic approaches. Immune cell engagers (ICEs) have emerged as promising strategies to redirect endogenous immune cells toward tumor targets. While conventional surface-antigen-directed engagers can bypass major histocompatibility complex (MHC)-restricted antigen presentation, peptide-HLA-directed constructs remain dependent on antigen processing, peptide presentation, and the appropriate HLA allele. More recently, tumor-restricted activation technologies, collectively referred to as "masking immunotherapy", have been developed to preferentially restore target and/or immune-cell engagement under TME-associated conditions, such as increased protease activity. These approaches have demonstrated enhanced tumor-preferential activity and/or improved tolerability in preclinical models; however, the extent to which these properties translate into clinically meaningful tumor selectivity and an improved therapeutic index remains under investigation. In this review, based on the authors' comprehensive literature knowledge and targeted searches, we critically examine the biological rationale, the engineering principles, the current preclinical evidence, and the early clinical development of ICEs with particular emphasis on their potential application in HNSCC. While early clinical studies have demonstrated acceptable safety profiles, clinical activity has generally been poor. We discuss the major barriers that limit clinical translation and we highlight emerging strategies aimed at overcoming these limitations. We believe that successful clinical development of ICEs in HNSCC will likely depend not only on the identification of optimal target antigens, but also on spatially controlled immune activation, integration with TME-modulating approaches, and incorporation of dynamic biomarkers into future clinical trial design. FAU - Nardo, Giuseppe AU - Nardo G AUID- ORCID: 0009-0006-2085-8882 AD - Head and Neck Medical Oncology Department, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy. FAU - Lenoci, Deborah AU - Lenoci D AUID- ORCID: 0000-0002-5958-8035 AD - Integrated Biology of Rare Tumors, Department of Experimental Oncology, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy. FAU - Lucchetta, Marta AU - Lucchetta M AD - Integrated Biology of Rare Tumors, Department of Experimental Oncology, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy. FAU - Licitra, Lisa AU - Licitra L AD - Head and Neck Medical Oncology Department, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy. AD - Department of Oncology and Hemato-Oncology, University of Milan, 20122 Milan, Italy. FAU - De Cecco, Loris AU - De Cecco L AUID- ORCID: 0000-0002-7066-473X AD - Integrated Biology of Rare Tumors, Department of Experimental Oncology, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy. FAU - Cavalieri, Stefano AU - Cavalieri S AUID- ORCID: 0000-0003-1294-6859 AD - Head and Neck Medical Oncology Department, Fondazione IRCCS Istituto Nazionale dei Tumori, 20133 Milan, Italy. AD - Department of Oncology and Hemato-Oncology, University of Milan, 20122 Milan, Italy. LA - eng GR - IG23573/Italian Association for Cancer Research/ PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13703286 OTO - NOTNLM OT - HNSCCs OT - ICE OT - NKCE OT - T-cell engagers OT - TCE OT - gamma-delta TCE OT - head and neck cancers OT - immune-cell engagers OT - masking immunotherapy OT - phagocytic-engager COIS- Stefano Cavalieri discloses occasional fees for participation as a speaker at conferences/congresses from AccMed; support for attending meetings and/or travel from AccMed, MultiMed Engineers srl, Care Insight sas, unrelated to the content of this work. Lisa Licitra discloses the following conflicts of interest: Research funds donated directly to the institute for clinical trials from Adlai Nortye; Astrazeneca; BMS; Debiopharm International SA; Eisai; Eli Lilly and Company; Exelixis; Hoffman-La Roche Ltd.; Isa Therapeutics; Kura Oncology; Merck Serono; MSD; Merck Sharp & Dome Corp; Nektar Therapeutics; Novartis; Regeneron; Roche; Sanofi; Syneos; Sun Pharmaceutica; Incyte Biosciences International Sarl; Gilead Sciences, Inc.; Genmab; Merck Healthcare KGaA. Occasional fees for, participation as a speaker at conferences/congresses or as a scientific consultant for advisory boards, from MSD IT; Merck Serono Spa Healthcare Professional; Merck Healthcare KGaA; GSK, F. Hoffman La Roche Ltd.; EMD Serono Research & Development Institute, Inc.; Boehringer Ingelhem International GmbH; Simon-Kucher & Partners Strategy & Marketing Consultants; Regenta Therapeutics, Inc; Alentis Therapeutics AG; MedImmune Limited. Advisory board participation at Merck Healthcare KGaA.; Merck & Co. Inc; a; MSD IT; EMD Serono Research & Development Institute, Inc.; F. Hoffman-La Roche Ltd.; Janssen Research & Development, LLC; Seagen International GmbH; Genmab US, Inc.; Astrazeneca UK limited; Abbvie Srl; Simon-Kucher & Partners Strategy & Marketing Consultants; Purple Biotech, Ltd.; LEO Pharma A/S. Travel grants from TAE Life Science. Support for presentations, speakers bureaus, manuscript writing or educational events from Merck Serono Spa; MSD IT; Merck Healthcare KGaA; Adlai Nortye; Bristol Mayers Squibb [K.K.]; ALTIS Omnia Pharma Service Srl. The other authors declare that they have no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/21 CRDT- 2026/10/27 10:32 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/20 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - cancers18203401 [pii] AID - cancers-18-03401 [pii] AID - 10.3390/cancers18203401 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 21;18(20):3401. doi: 10.3390/cancers18203401. PMID- 42938932 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 21 TI - Targeting Peripheral T-Cell Lymphomas with Chimeric Antigen Receptor Therapy: Mechanistic Challenges, Clinical Landscape and Future Engineering Perspectives. LID - 10.3390/cancers18203400 [doi] LID - 3400 AB - Peripheral T-cell lymphomas (PTCL) comprise a biologically diverse and clinically aggressive family of mature T/NK-cell neoplasms, for which outcomes remain poor in the majority of patients treated with conventional anthracycline-based frontline regimens. Contemporary classifications (WHO-HAEM5 and ICC) increasingly incorporate immunophenotypic and molecular information, underscoring that "PTCL-NOS" is not a single disease but rather a diagnostic umbrella that includes biologically distinct entities and molecular subgroups. Among these are the TBX21- and GATA3-driven PTCL-NOS subsets, the latter associated with inferior survival and a distinctive cytokine/microenvironmental program. These advances, many of which were shaped by long-standing contributions from the Bologna group, support a rational shift from empiric chemotherapy toward mechanism-based and immune-based interventions. CAR-engineered cellular therapies have revolutionized the care of B-cell malignancies, yet translation to PTCL has been delayed by lineage-specific barriers: (i) fratricide during manufacturing when targeting shared T-cell antigens (e.g., CD5, CD7), (ii) the risk of profound and prolonged T-cell aplasia (a markedly less "replaceable" toxicity than B-cell aplasia), and (iii) product contamination by malignant T-cells in autologous platforms. Recent progress in genome editing (CRISPR/Cas9 and base editing), intracellular antigen "blocking" strategies, and alternative effectors (allogeneic "universal" CAR-T, CAR-NK, and iPSC-derived platforms) has begun to address these obstacles at the manufacturing level, while their downstream clinical consequences (e.g., prolonged cytopenias, infectious risk and delayed immune reconstitution) remain incompletely resolved. Early-phase clinical trials now demonstrate clinically meaningful responses for select targets, particularly CD7, CD5, TRBC1 and CD30, for the pan-T targets. However, the strongest efficacy data remain largely derived from T-cell lymphoblastic malignancies (T-ALL/T-LBL), whereas evidence in mature PTCL remains limited and at an earlier stage, with target-specific differences in safety, immune reconstitution and relapse patterns. This review synthesizes the biological rationale for antigen selection in PTCL, the emerging clinical evidence for leading CAR targets, and the engineering strategies most likely to improve the therapeutic index. It also proposes an integrated hematology perspective on how CAR-based therapies might be positioned relative to transplant and evolving targeted agents in relapsed/refractory PTCL. FAU - Irimia, Ruxandra AU - Irimia R AD - "Agrippa Ionescu" Emergency Hospital, 1 Arh. Ion Mincu Street, 077015 Bucharest, Romania. FAU - Piccaluga, Pier Paolo AU - Piccaluga PP AUID- ORCID: 0000-0002-9432-0595 AD - Biobank of Research, IRCCS Azienda Ospedaliero-Universitaria di Bologna, Via Massarenti, 9, 40138 Bologna, Italy. AD - Department of Medical and Surgical Sciences, Institute of Hematology and Medical Oncology "L&A Seragnoli", Bologna University School of Medicine, University of Bologna, Via Massarenti, 9, 40138 Bologna, Italy. LA - eng GR - RC-2026-2801358/Governo Italiano/ PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702787 OTO - NOTNLM OT - CAR-NK OT - CAR-T OT - CD30 OT - CD5 OT - CD7 OT - PTCL-NOS OT - TRBC1 OT - fratricide OT - genome editing OT - peripheral T-cell lymphoma OT - tumor microenvironment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/21 CRDT- 2026/10/27 10:32 PHST- 2026/05/29 00:00 [received] PHST- 2026/08/31 00:00 [revised] PHST- 2026/09/10 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - cancers18203400 [pii] AID - cancers-18-03400 [pii] AID - 10.3390/cancers18203400 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 21;18(20):3400. doi: 10.3390/cancers18203400. PMID- 42938931 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 21 TI - Salvage Surgery with Intraoperative Electron Radiation Therapy for Recurrent or Persistent Head and Neck Cancer After Prior Radiotherapy. LID - 10.3390/cancers18203399 [doi] LID - 3399 AB - Background/Objectives: Locoregionally recurrent or persistent head and neck cancer (rpHNC) after prior radiotherapy carries a poor prognosis, and optimal salvage strategies remain uncertain. We evaluated survival, patterns of failure, and 90-day postoperative complications after salvage surgery combined with intraoperative electron radiation therapy (IOERT). Methods: We retrospectively identified 127 previously irradiated patients treated with salvage surgery and IOERT between 1999 and 2023. Overall survival (OS) and disease-free survival (DFS) were estimated using the Kaplan-Meier method, and locoregional failure (LRF) and distant failure were estimated using Aalen-Johansen cumulative incidence with death as a competing event. Associations were evaluated one predictor at a time in age- and sex-adjusted Cox (OS, DFS) and Fine-Gray (LRF) models without multiplicity adjustment, and they are exploratory. Results: With a median follow-up of 70 months, median overall survival (OS) was 20.5 months (95% CI, 14.6-40.2), with 1-, 2-, and 3-year OS rates of 63%, 48%, and 43%, respectively. Median disease-free survival (DFS) was 8.8 months. Positive surgical margins and extranodal extension (ENE) were associated with worse OS in separate age- and sex-adjusted models. The 1-, 2-, and 3-year cumulative incidences of LRF were 34.3%, 44.7%, and 49.8%, respectively. The corresponding cumulative incidences of distant failure were 21.6%, 24.0%, and 26.7%, respectively. Thirty-seven patients had a documented distant failure, of whom 27 (73%) experienced distant failure within one year. Available records documented three deaths within 90 days (3/127, 2.4%). Conclusions: Among rpHNC patients treated with this IOERT-containing multimodality salvage strategy, median OS was 20.5 months. Because the study lacked a salvage-surgery-only comparator, postoperative treatments were selectively administered, and complication reporting was incomplete, neither the efficacy nor the comparative safety of IOERT can be determined from these data. FAU - Elguindy, Ahmed N AU - Elguindy AN AD - Department of Radiation Oncology, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. AD - Department of Radiation Oncology, El-Demerdash Hospitals, Ain Shams University, Cairo 11566, Egypt. FAU - Baliga, Sujith AU - Baliga S AD - Department of Radiation Oncology, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. AD - Department of Radiation Oncology, Winship Cancer Institute of Emory University, Emory University School of Medicine, Atlanta, GA 30322, USA. FAU - Klamer, Brett G AU - Klamer BG AD - Center for Biostatistics, College of Medicine, The Ohio State University, Columbus, OH 43210, USA. FAU - Palmiero, Allison AU - Palmiero A AD - Department of Radiation Oncology, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - Dibs, Khaled N AU - Dibs KN AD - Department of Radiation Oncology, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - Koempel, Andrew AU - Koempel A AD - Department of Radiation Oncology, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - Gogineni, Emile AU - Gogineni E AUID- ORCID: 0000-0001-6222-311X AD - Department of Radiation Oncology, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - Konieczkowski, David AU - Konieczkowski D AD - Department of Radiation Oncology, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - Mitchell, Darrion L AU - Mitchell DL AUID- ORCID: 0000-0003-1733-9644 AD - Department of Radiation Oncology, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - Seim, Nolan AU - Seim N AUID- ORCID: 0000-0002-9227-5313 AD - Department of Otolaryngology-Head and Neck Surgery, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - VanKoevering, Kyle AU - VanKoevering K AD - Department of Otolaryngology-Head and Neck Surgery, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - Haring, Catherine AU - Haring C AD - Department of Otolaryngology-Head and Neck Surgery, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - Miller, Lauren AU - Miller L AD - Department of Otolaryngology-Head and Neck Surgery, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - Kang, Stephen Y AU - Kang SY AD - Department of Otolaryngology-Head and Neck Surgery, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - Agrawal, Amit AU - Agrawal A AUID- ORCID: 0000-0003-4047-5708 AD - Department of Otolaryngology-Head and Neck Surgery, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - Ozer, Enver AU - Ozer E AD - Department of Otolaryngology-Head and Neck Surgery, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - Rocco, James W AU - Rocco JW AD - Department of Otolaryngology-Head and Neck Surgery, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - Carrau, Ricardo L AU - Carrau RL AD - Department of Otolaryngology-Head and Neck Surgery, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - Old, Matthew AU - Old M AD - Department of Otolaryngology-Head and Neck Surgery, University of Washington, Seattle, WA 98195, USA. FAU - Bonomi, Marcelo AU - Bonomi M AUID- ORCID: 0000-0002-9399-3608 AD - Department of Medical Oncology, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - Bhateja, Priyanka AU - Bhateja P AUID- ORCID: 0000-0001-9515-0498 AD - Department of Medical Oncology, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - Chakravarti, Arnab AU - Chakravarti A AUID- ORCID: 0000-0002-5392-8721 AD - Department of Radiation Oncology, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - Grecula, John AU - Grecula J AUID- ORCID: 0009-0007-1817-063X AD - Department of Radiation Oncology, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. FAU - Blakaj, Dukagjin M AU - Blakaj DM AD - Department of Radiation Oncology, The James Cancer Hospital/Wexner Medical Center, The Ohio State University, Columbus, OH 43210, USA. AD - Adjunct Faculty of Medicine, University of Prishtina, St. George Bush, No. 31, 10000 Prishtina, Kosovo. LA - eng PT - Journal Article DEP - 20261021 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702705 OTO - NOTNLM OT - head and neck cancer OT - immunotherapy OT - intraoperative electron radiation therapy OT - locoregional failure OT - recurrent cancer OT - reirradiation OT - salvage surgery OT - treatment toxicity COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/21 CRDT- 2026/10/27 10:32 PHST- 2026/08/13 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - cancers18203399 [pii] AID - cancers-18-03399 [pii] AID - 10.3390/cancers18203399 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 21;18(20):3399. doi: 10.3390/cancers18203399. PMID- 42938930 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 21 TI - Clonal Hematopoiesis Across the Cancer Treatment Continuum: A Scoping Review of Clonal Selection, Evolution, and Clinical Outcomes. LID - 10.3390/cancers18203398 [doi] LID - 3398 AB - BACKGROUND: Clonal hematopoiesis (CH) is characterized by the proliferation of blood cell clones that possess acquired genetic modifications. While therapy-related CH refers to CH detected in the setting of prior or current treatment in cancer populations, therapy-selected CH demands demonstration that treatment preferentially expanded or maintained an extant clone; therapy-induced CH requires evidence of de novo treatment-associated mutation supported by serial sampling and mutational-signature analyses. OBJECTIVE: To map evidence distinguishing therapy-related, therapy-selected and therapy-induced CH across cancer treatment, including clonal dynamics, genomic features, clinical outcomes and surveillance gaps. METHODS: Following Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) guidance, PubMed/MEDLINE, Embase, Scopus, Web of Science Core Collection, Cochrane Library and Google Scholar were searched from inception through 1 June 2026, and updated on 15 September 2026. Primary evidence mapping was limited to original human studies. Reviews and conceptual sources were used only for context or reference tracking and were ineligible for the primary evidence map. Narrative synthesis was employed as critical variation existed in sequencing platforms, variant allele frequency thresholds, timepoints of sampling, filtering strategies, and outcome definitions. RESULTS: Of 684 records identified, 213 duplicates were removed and 471 records were screened. Across both pathways, 121 full texts were assessed, 87 excluded and 34 primary reports mapped; contextual citations were outside the included set. Contextual references were used for definitions and interpretation and were excluded from the included-report count. Six domains were deductively established and inductively refined during charting: terminology; therapy-associated mechanisms of clonal selection/expansion; genomic architecture; longitudinal clonal dynamics; clinical consequences; and study design and methodological heterogeneity. Treatment-associated selection or expansion of pre-existing TP53 and PPM1D clones was the most consistently reported mechanism of therapy-related CH, while CHEK2 was inconsistently detected. Pediatric cohorts and mutational-signature analyses also supported de novo mutagenesis with therapy in select contexts. Progression to therapy-related myeloid neoplasm was the most consistent outcome across studies; however, cytopenia, CAR-T-therapy-associated toxicity, cardiovascular events and mortality were inconsistently reported. CONCLUSION: Current evidence supports a spectrum of mechanisms ranging from selection of pre-existing clones to therapy-induced de novo mutation. Existing literature cannot support universal CH screening recommendations or guide treatment decisions. Prospective serial analyses harmonized on definition, employing sensitive baseline assays with paired germline controls and tumor-contamination checks, are needed to justify clinical implementation and assessment of fixed outcomes. FAU - AlShahrani, Abdullah M AU - AlShahrani AM AUID- ORCID: 0000-0002-9433-1913 AD - Department of Basic Medical Science, College of Applied Medical Sciences, King Khalid University (KKU), Abha 62561, Saudi Arabia. LA - eng GR - RGP1/18/47/King Khalid University/ PT - Journal Article PT - Review DEP - 20261021 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702734 OTO - NOTNLM OT - CHIP OT - PPM1D OT - PRISMA-ScR OT - TP53 OT - cancer survivorship OT - clonal hematopoiesis OT - therapy-related clonal hematopoiesis OT - therapy-related myeloid neoplasms OT - therapy-selected clonal hematopoiesis COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/21 CRDT- 2026/10/27 10:32 PHST- 2026/09/10 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/21 00:00 [pmc-release] AID - cancers18203398 [pii] AID - cancers-18-03398 [pii] AID - 10.3390/cancers18203398 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 21;18(20):3398. doi: 10.3390/cancers18203398. PMID- 42938929 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 20 TI - Measurable Residual Disease (MRD) Monitoring in Myeloproliferative Neoplasms. LID - 10.3390/cancers18203397 [doi] LID - 3397 AB - BCR::ABL1 negative myeloproliferative neoplasms (MPN) are driven predominantly by somatic mutations in JAK2, CALR, and MPL, which underpin diagnosis, prognostication, and measurable residual disease (MRD) monitoring. Advances in molecular assays have improved sensitivity for detecting canonical drivers and additional mutations across the MPN spectrum. In this review we evaluate current and emerging molecular methodologies for polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (PMF). We review the utility of PCR-based approaches, including allele-specific PCR, real-time quantitative PCR, and droplet digital PCR (ddPCR). Advances in ddPCR demonstrate precision and lower limits of detection for JAK2 V617F, CALR, and non-driver mutations, thus supporting the expanding role of ddPCR in MRD monitoring and relapse prediction. Recent advances in NGS techniques provide targeted profiling, enabling identification of possible co-occurring driver mutations and additional mutations associated with disease progression, treatment resistance, and leukemic transformation. Collectively, emerging workflows complement established gold-standard methodologies, while offering enhanced analytical performance and deeper pathological insight across the MPN spectrum. Optimal method selection requires balancing assay sensitivity, cost, and clinical utility. Advances in ddPCR and NGS methodologies have applicability for MRD monitoring in MPN and are powerful tools for detection of heterogenous and co-occurrent mutations across the MPN spectrum. FAU - Markey, Gary E AU - Markey GE AD - Cancer Molecular Diagnostics, St. James's Hospital, 8 Dublin Dublin, Ireland. FAU - Langabeer, Stephen E AU - Langabeer SE AD - Cancer Molecular Diagnostics, St. James's Hospital, 8 Dublin Dublin, Ireland. LA - eng PT - Journal Article PT - Review DEP - 20261020 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702316 OTO - NOTNLM OT - CALR OT - JAK2 OT - MPL OT - NGS OT - ddPCR OT - essential thrombocythemia OT - myeloproliferative neoplasm OT - polycythemia vera OT - primary myelofibrosis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/20 CRDT- 2026/10/27 10:32 PHST- 2026/09/28 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - cancers18203397 [pii] AID - cancers-18-03397 [pii] AID - 10.3390/cancers18203397 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 20;18(20):3397. doi: 10.3390/cancers18203397. PMID- 42938928 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 20 TI - The SP4-NUPR1-SELM Axis Promotes Glycolytic Reprogramming and Stemness-Associated Phenotypes in Bladder Cancer. LID - 10.3390/cancers18203396 [doi] LID - 3396 AB - Background: Metabolic adaptation and stemness-associated programs contribute to bladder cancer growth. This study investigated the regulatory and functional relationships among specificity protein 4 (SP4), nuclear protein 1 (NUPR1), and selenoprotein M (SELM). Methods: TCGA-BLCA analyses were combined with silencing, overexpression, and rescue experiments in 5637 and T24 cells. Viability, migration, invasion, colony formation, glucose uptake, lactate production, extracellular acidification, oxygen consumption, and OCT4/SOX2 expression were assessed. Protein interactions and promoter-associated regulation were examined using co-immunoprecipitation, immunofluorescence, chromatin immunoprecipitation, and reporter assays. Xenograft experiments in female BALB/c nude mice evaluated NUPR1 depletion with or without SP4 overexpression. Results: Higher NUPR1 expression was associated with higher glycolysis- and stemness-associated transcriptional scores in TCGA-BLCA tumors. NUPR1 depletion reduced viability, glycolytic activity, glucose uptake, lactate production, and OCT4/SOX2 expression while increasing oxygen consumption. SP4 interacted with NUPR1 and showed enrichment at the NUPR1 promoter region. NUPR1 promoted SELM expression and reporter activity, with enrichment detected at the SELM promoter region. SP4 or SELM overexpression partially rescued the effects of NUPR1 depletion. SELM silencing attenuated NUPR1-associated increases in viability, migration, invasion, and colony formation. NUPR1 depletion suppressed xenograft growth, which was partially rescued by SP4 overexpression. Conclusions: These findings support functional relationships among SP4, NUPR1, and SELM that connect glycolytic activity with stemness-associated phenotypes in bladder cancer models. The results provide a basis for further investigation of this regulatory network in cancer metabolism. FAU - Deng, Hannuo AU - Deng H AD - Department of Urology & Urology Laboratory, The Third Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China. FAU - Sun, Rui AU - Sun R AD - Department of Urology & Urology Laboratory, The Third Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China. FAU - He, Guangming AU - He G AD - Department of Urology & Urology Laboratory, The Third Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China. FAU - Tan, Yuchen AU - Tan Y AD - Department of Urology & Urology Laboratory, The Third Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China. FAU - Zhang, Lei AU - Zhang L AUID- ORCID: 0000-0002-7988-7585 AD - Department of Urology & Urology Laboratory, The Third Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China. FAU - Gao, Shenglin AU - Gao S AD - Department of Urology & Urology Laboratory, The Third Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China. FAU - Zhu, Yichao AU - Zhu Y AUID- ORCID: 0000-0003-3499-8510 AD - Department of Physiology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, 211166, China. FAU - Zhang, Lifeng AU - Zhang L AD - Department of Urology & Urology Laboratory, The Third Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China. FAU - Zuo, Li AU - Zuo L AD - Department of Urology & Urology Laboratory, The Third Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China. FAU - Tang, Zhiqi AU - Tang Z AD - Department of Radiology, The Third Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China. LA - eng GR - No. ZD202440/Science and Technology Project of Changzhou Health Commission/ GR - 2022CZBJ057/Top Talent of Changzhou "The 14th Five-Year Plan" High-Level Health Talents Training Project/ GR - Y-CXNLMS2026-0004/CSCO-Innovative Research Project on Urothelial Carcinoma/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702499 OTO - NOTNLM OT - NUPR1 OT - SELM OT - SP4 OT - bladder cancer OT - glycolysis OT - metabolic reprogramming OT - stemness OT - tumor progression COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/20 CRDT- 2026/10/27 10:32 PHST- 2026/09/23 00:00 [received] PHST- 2026/10/16 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - cancers18203396 [pii] AID - cancers-18-03396 [pii] AID - 10.3390/cancers18203396 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 20;18(20):3396. doi: 10.3390/cancers18203396. PMID- 42938925 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 20 TI - Drug Delivery Systems for Breast Cancer Treatment: From Design Principles to Clinical Translation. LID - 10.3390/cancers18203393 [doi] LID - 3393 AB - BACKGROUND/OBJECTIVES: Breast cancer is the most frequently diagnosed malignancy worldwide and remains a major cause of cancer-related mortality among women. Despite advances in screening, molecular classification, and systemic therapies, treatment of advanced and metastatic disease remains challenging because of tumor heterogeneity, multidrug resistance, systemic toxicity, limited drug selectivity, and inadequate penetration into the tumor microenvironment. This review aims to critically evaluate advanced drug delivery systems (DDSs) for breast cancer therapy, emphasizing the relationship between carrier design, tumor biology, therapeutic performance, and clinical translation. METHODS: The recent literature on DDSs for breast cancer treatment was comprehensively reviewed, covering liposomes, polymeric nanoparticles and micelles, dendrimers, nanogels, lipid- and inorganic-based nanoparticles, extracellular vesicles, biomimetic platforms, and stimuli-responsive systems. Particular attention was given to active and passive targeting, controlled drug release, strategies for overcoming multidrug resistance and biological barriers, and combination approaches integrating chemotherapy with immunotherapy, gene therapy, and phototherapy. Clinically approved and clinical-stage nanomedicines were also considered. RESULTS: Advanced DDSs can improve drug stability, pharmacokinetics, biodistribution, tumor accumulation, cellular uptake, and controlled release, potentially enhancing therapeutic efficacy while reducing systemic toxicity. Targeted, biomimetic, and stimuli-responsive platforms provide additional opportunities to address tumor heterogeneity, the tumor microenvironment, and therapeutic resistance. Several DDS-based nanomedicines have reached clinical use or advanced clinical development, demonstrating their translational potential. However, manufacturing scalability, reproducibility, long-term safety, regulatory requirements, and patient heterogeneity remain important barriers. CONCLUSIONS: Advanced DDSs represent promising tools for improving the precision and efficacy of breast cancer treatment. Future progress will require biologically informed carrier design, reproducible manufacturing, rigorous clinical validation, and patient stratification. Integrating nanomedicine with precision oncology may accelerate the clinical translation of next-generation DDSs and support more effective personalized therapeutic strategies. FAU - Sescu, Vasile Adrian AU - Sescu VA AUID- ORCID: 0009-0002-6336-4883 AD - Department of Obstetrics and Gynecology, Faculty of General Medecine, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. FAU - Condriuc, Iustina Petra Solomon AU - Condriuc IPS AD - Department of Obstetrics and Gynecology, Faculty of General Medecine, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. FAU - Spridon, Liana Gabriela AU - Spridon LG AD - Cristofor Simionescu Faculty of Chemical Engineering and Environmental Protection, Gheorghe Asachi Technical University of Iasi, 700050 Iasi, Romania. AD - Faculty of Medicine, Apollonia University of Iasi, 700055 Iasi, Romania. FAU - Cadinoiu, Anca Niculina AU - Cadinoiu AN AUID- ORCID: 0000-0002-3708-3992 AD - Faculty of Medicine, Apollonia University of Iasi, 700055 Iasi, Romania. FAU - Miron, Lucian AU - Miron L AD - Faculty of Medicine, Apollonia University of Iasi, 700055 Iasi, Romania. FAU - Atanase, Leonard Ionut AU - Atanase LI AUID- ORCID: 0000-0002-3657-1492 AD - Faculty of Medicine, Apollonia University of Iasi, 700055 Iasi, Romania. AD - Academy of Romanian Scientists, 050044 Bucharest, Romania. FAU - Matasariu, Roxana Daniela AU - Matasariu RD AUID- ORCID: 0000-0002-2862-2069 AD - Department of Obstetrics and Gynecology, Faculty of General Medecine, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. FAU - Socolov, Demetra Gabriela AU - Socolov DG AUID- ORCID: 0000-0002-0979-0200 AD - Department of Obstetrics and Gynecology, Faculty of General Medecine, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. FAU - Carauleanu, Alexandru AU - Carauleanu A AUID- ORCID: 0000-0001-9112-7039 AD - Department of Obstetrics and Gynecology, Faculty of General Medecine, Grigore T. Popa University of Medicine and Pharmacy, 700115 Iasi, Romania. LA - eng PT - Journal Article PT - Review DEP - 20261020 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702740 OTO - NOTNLM OT - breast cancer OT - clinical translation OT - drug delivery systems OT - nanocarriers OT - nanomedicine OT - targeted drug delivery COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/20 CRDT- 2026/10/27 10:32 PHST- 2026/09/07 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - cancers18203393 [pii] AID - cancers-18-03393 [pii] AID - 10.3390/cancers18203393 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 20;18(20):3393. doi: 10.3390/cancers18203393. PMID- 42938921 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 20 TI - FragMIL: A Fragment-Token Multiple-Instance Learning Framework for Cancer Detection from Cell-Free DNA Fragmentomics. LID - 10.3390/cancers18203389 [doi] LID - 3389 AB - Background/Objectives: Cell-free DNA (cfDNA) fragmentomics from low-coverage whole-genome sequencing (lcWGS) is a promising approach for non-invasive cancer detection. However, most existing methods summarize millions of cfDNA fragments into handcrafted sample-level features before classification, potentially discarding informative patterns carried by individual molecules. We aimed to develop and benchmark a framework that learns directly from fragment-level signals. Methods: We developed FragMIL, a fragment-token multiple-instance learning framework that represents each cfDNA molecule as a learnable token integrating end-motif, genomic-position, and fragment-length information, and aggregates thousands of fragment tokens into a patient-level cancer score. FragMIL was evaluated on 934 samples from three public lcWGS cohorts (pan-cancer, lung cancer, and hepatocellular carcinoma) using five repeated subject-level splits, a 288-configuration design-space benchmark, and comparison with four established fragmentomic approaches (coverage, DELFI, end motif, and fragment size). Results: FragMIL achieved the highest mean areas under the receiver operating characteristic curve (AUROCs) in every cohort, with 0.963 +/- 0.009, 0.802 +/- 0.009, and 0.910 +/- 0.015 in the DELFI/Cristiano 2019, LUCAS/Mathios 2021, and Jiang 2015 cohorts, respectively. End-motif encoding provided the strongest standalone discriminative signal, whereas the optimal fragment pool was cohort-dependent, with open chromatin region fragments performing best for pan-cancer detection and tumor-enriched fragments for disease-specific cohorts. Performance was retained across cancer stages and cancer types. Conclusions: Preserving molecule-level fragmentomic information improves cancer detection, supporting fragment-token learning as a promising framework for next-generation cfDNA fragmentomics. FAU - Bui, Tien-Dung AU - Bui TD AD - International Ph.D. Program in Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan. AD - Department of Pathology and Laboratory Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei 111, Taiwan. AD - AIBioMed Lab, Taipei Medical University, Taipei 110, Taiwan. FAU - Lin, Yung-Feng AU - Lin YF AUID- ORCID: 0000-0002-6961-3687 AD - Department of Internal Medicine, Taipei Medical University Hospital, Taipei Medical University, Taipei 110, Taiwan. AD - Taipei Cancer Center, Taipei Medical University Hospital, Taipei Medical University, Taipei 110, Taiwan. FAU - Nguyen, Mai-Hanh AU - Nguyen MH AUID- ORCID: 0000-0002-4945-1334 AD - AIBioMed Lab, Taipei Medical University, Taipei 110, Taiwan. AD - Department of Pathology, 103 Military Hospital, Hanoi 100000, Vietnam. FAU - Tsai, Shih-Feng AU - Tsai SF AD - Institute of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli 350, Taiwan. FAU - Le, Nguyen-Quoc-Khanh AU - Le NQ AUID- ORCID: 0000-0003-4896-7926 AD - AIBioMed Lab, Taipei Medical University, Taipei 110, Taiwan. AD - In-Service Master Program in Artificial Intelligence in Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan. AD - Translational Imaging Research Center, Taipei Medical University Hospital, Taipei 110, Taiwan. FAU - Chou, Teh-Ying AU - Chou TY AD - Department of Pathology and Laboratory Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei 111, Taiwan. AD - Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, Taipei 110, Taiwan. LA - eng GR - 115-2320-B-341-002/National Science and Technology Council/ PT - Journal Article DEP - 20261020 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702332 OTO - NOTNLM OT - cancer detection OT - cell-free DNA OT - deep learning OT - fragmentomics OT - liquid biopsy OT - multiple-instance learning COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/20 CRDT- 2026/10/27 10:32 PHST- 2026/09/18 00:00 [received] PHST- 2026/10/18 00:00 [revised] PHST- 2026/10/19 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/20 00:00 [pmc-release] AID - cancers18203389 [pii] AID - cancers-18-03389 [pii] AID - 10.3390/cancers18203389 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 20;18(20):3389. doi: 10.3390/cancers18203389. PMID- 42938918 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 20 TI - Outcomes of Combined Hepatectomy and Intraoperative Ablation for Multiple Colorectal Liver Metastases: A Single-Center Retrospective Study. LID - 10.3390/cancers18203386 [doi] LID - 3386 AB - Introduction: Radical hepatectomy is the standard curative treatment for colorectal liver metastases (CRLMs). However, extensive resection may be contraindicated in patients with insufficient liver reserve or anatomically complex disease. Although ablation therapy is generally not recommended as a primary curative modality in current guidelines, intraoperative ablation may facilitate parenchymal-sparing, curative-intent treatment in selected high-risk patients. Methods: This retrospective cohort study included 118 patients who underwent curative-intent hepatectomy for multiple CRLM. Patients were categorized into hepatectomy-only (Hx) and hepatectomy plus intraoperative ablation (Hx + Abl) groups, and propensity score matching (PSM) generated 29 balanced pairs. Overall survival (OS) and hepatic progression-free survival (HPFS) were compared using a log-rank test. Cox regression identified predictors of OS, and receiver operating characteristic (ROC) analysis evaluated tumor size associated with local recurrence after ablation. Results: Before PSM, the HX + Abl group showed higher tumor burden and Beppu scores, and demonstrated inferior OS and HPFS. After PSM, oncological outcomes were comparable. Perioperative outcomes, including major complications and post-hepatectomy liver failure (PHLF), were similar. Preoperative chemotherapy was an independent predictor of OS. Among 99 ablated tumors, local recurrence occurred in 12 lesions, and ROC analysis identified tumor size /= 0.11), nor did composite translational (p = 0.27) or rotational (p = 0.70) errors. SGRT showed a mean vertical offset of -5.1 mm relative to control (95% CI -8.9 to -1.3 mm; p = 0.013, Bonferroni-adjusted p = 0.078). Clinically significant pre-correction deviations (>/=5 mm) occurred in 52-65% of fractions in the vertical and longitudinal directions in both cohorts. Mean preparation-for-treatment time was shorter with SGRT (11.0 vs. 13.9 min; median 10.0 vs. 12.5 min; p = 0.036). Conclusions: Initial setup with ExacTrac Dynamic showed no statistically significant difference in setup accuracy compared with tattoo-based setup and shortened preparation time but was associated with a vertical offset. Pre-correction errors were large with both methods, so daily image guidance remains necessary. Because backup tattoos were placed in all patients and equivalence was not formally tested, these results support-but do not establish-tattoo-free daily setup. Prospective, multicenter studies are warranted to validate these findings and assess long-term clinical outcomes. FAU - Krayim, Bilal AU - Krayim B AUID- ORCID: 0009-0005-8338-5720 AD - Helmsley Cancer Center-Shaare Zedek Medical Center, The Hebrew University of Jerusalem, Shmuel Bait 12, Jerusalem 9103102, Israel. FAU - Yaacov, Adar AU - Yaacov A AD - Helmsley Cancer Center-Shaare Zedek Medical Center, The Hebrew University of Jerusalem, Shmuel Bait 12, Jerusalem 9103102, Israel. FAU - Abu Myala, Mohammad AU - Abu Myala M AD - Helmsley Cancer Center-Shaare Zedek Medical Center, The Hebrew University of Jerusalem, Shmuel Bait 12, Jerusalem 9103102, Israel. FAU - Awad, Muhammad AU - Awad M AD - Helmsley Cancer Center-Shaare Zedek Medical Center, The Hebrew University of Jerusalem, Shmuel Bait 12, Jerusalem 9103102, Israel. FAU - Kahla, Dolev AU - Kahla D AD - Helmsley Cancer Center-Shaare Zedek Medical Center, The Hebrew University of Jerusalem, Shmuel Bait 12, Jerusalem 9103102, Israel. FAU - Afifi, Basel AU - Afifi B AD - Helmsley Cancer Center-Shaare Zedek Medical Center, The Hebrew University of Jerusalem, Shmuel Bait 12, Jerusalem 9103102, Israel. FAU - Dabbah, Basil AU - Dabbah B AD - Helmsley Cancer Center-Shaare Zedek Medical Center, The Hebrew University of Jerusalem, Shmuel Bait 12, Jerusalem 9103102, Israel. FAU - Gillis, Roni AU - Gillis R AUID- ORCID: 0000-0001-6226-2573 AD - Helmsley Cancer Center-Shaare Zedek Medical Center, The Hebrew University of Jerusalem, Shmuel Bait 12, Jerusalem 9103102, Israel. FAU - Grinshpun, Albert AU - Grinshpun A AD - Helmsley Cancer Center-Shaare Zedek Medical Center, The Hebrew University of Jerusalem, Shmuel Bait 12, Jerusalem 9103102, Israel. FAU - Abu Ramila, Areen AU - Abu Ramila A AD - Helmsley Cancer Center-Shaare Zedek Medical Center, The Hebrew University of Jerusalem, Shmuel Bait 12, Jerusalem 9103102, Israel. FAU - Abramovich, Amir AU - Abramovich A AD - Helmsley Cancer Center-Shaare Zedek Medical Center, The Hebrew University of Jerusalem, Shmuel Bait 12, Jerusalem 9103102, Israel. FAU - Rosenfield, Hila AU - Rosenfield H AD - Helmsley Cancer Center-Shaare Zedek Medical Center, The Hebrew University of Jerusalem, Shmuel Bait 12, Jerusalem 9103102, Israel. FAU - Honig, Nir AU - Honig N AD - Helmsley Cancer Center-Shaare Zedek Medical Center, The Hebrew University of Jerusalem, Shmuel Bait 12, Jerusalem 9103102, Israel. FAU - Koren, Sion AU - Koren S AD - Helmsley Cancer Center-Shaare Zedek Medical Center, The Hebrew University of Jerusalem, Shmuel Bait 12, Jerusalem 9103102, Israel. FAU - Rigler, Sharon AU - Rigler S AD - Helmsley Cancer Center-Shaare Zedek Medical Center, The Hebrew University of Jerusalem, Shmuel Bait 12, Jerusalem 9103102, Israel. FAU - Pfeffer, Raphael AU - Pfeffer R AD - Helmsley Cancer Center-Shaare Zedek Medical Center, The Hebrew University of Jerusalem, Shmuel Bait 12, Jerusalem 9103102, Israel. FAU - Allen, Aaron M AU - Allen AM AD - Helmsley Cancer Center-Shaare Zedek Medical Center, The Hebrew University of Jerusalem, Shmuel Bait 12, Jerusalem 9103102, Israel. LA - eng PT - Journal Article DEP - 20261019 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702465 OTO - NOTNLM OT - ExacTrac Dynamic OT - breast cancer OT - breast radiotherapy OT - image-guided radiotherapy (IGRT) OT - patient setup accuracy OT - setup reproducibility OT - surface-guided radiation therapy (SGRT) OT - tattoo-free positioning OT - treatment workflow efficiency COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/19 CRDT- 2026/10/27 10:32 PHST- 2026/09/09 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/19 00:00 [pmc-release] AID - cancers18203375 [pii] AID - cancers-18-03375 [pii] AID - 10.3390/cancers18203375 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 19;18(20):3375. doi: 10.3390/cancers18203375. PMID- 42938902 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 18 TI - Clinical and Biological Correlates of Resistance and Relapse After CD19-Directed CAR T-Cell Therapy in Large B-Cell Lymphoma: A Structured Narrative Review. LID - 10.3390/cancers18203370 [doi] LID - 3370 AB - BACKGROUND/OBJECTIVES: CD19-directed chimeric antigen receptor (CAR) T-cell therapy has transformed the treatment of relapsed/refractory large B-cell lymphoma (r/r LBCL), yet primary resistance and relapse remain common. We evaluated how recent clinical and translational evidence refines established resistance frameworks and which knowledge gaps prevent the relative contribution of individual mechanisms from being determined. METHODS: We conducted a structured narrative review of 14 selected clinical and translational studies addressing tumor-, product-, host-, and microenvironment-related dimensions of treatment failure, synthesized across four interpretive domains and compared with nine previous reviews. RESULTS: Durable benefit was associated with favorable tumor and immune transcriptional programs and greater CAR T-cell fitness, expansion, or persistence. Treatment failure was associated with tumor-intrinsic resistance programs, exhausted T-cell states, systemic inflammatory signals, and suppressive tumor-microenvironment features, including PD-L1-expressing macrophages. Antigen escape remained relevant, but incomplete serial CD19 assessment precluded conclusions about its relative contribution. Comparison with earlier reviews showed continuity rather than replacement, with LBCL-focused evidence adding biological resolution. CONCLUSIONS: Resistance and relapse after CD19 CAR T-cell therapy in LBCL are multifactorial. Prospective longitudinal studies integrating tumor tissue, apheresis and manufactured products, peripheral blood, and matched relapse samples are needed to validate predictive biomarkers and guide rational treatment strategies. FAU - Resende, Jean Silva de Souza AU - Resende JSS AD - Instituto de Pesquisa Pele Pequeno Principe, Curitiba 80250-060, PR, Brazil. AD - Faculdades Pequeno Principe, Reboucas, Curitiba 80230-020, PR, Brazil. AD - Bioinformatics and Systems Biology Laboratory, Federal University of Parana, Curitiba 81520-260, PR, Brazil. FAU - Giner, Igor Samesima AU - Giner IS AD - Instituto de Pesquisa Pele Pequeno Principe, Curitiba 80250-060, PR, Brazil. AD - Faculdades Pequeno Principe, Reboucas, Curitiba 80230-020, PR, Brazil. FAU - Custodio de Figueiredo, Maria Gabriela AU - Custodio de Figueiredo MG AUID- ORCID: 0009-0009-1516-6971 AD - Instituto de Pesquisa Pele Pequeno Principe, Curitiba 80250-060, PR, Brazil. FAU - Paraizo, Mariana Martins AU - Paraizo MM AUID- ORCID: 0000-0001-8866-5776 AD - Instituto de Pesquisa Pele Pequeno Principe, Curitiba 80250-060, PR, Brazil. FAU - Lalli, Enzo AU - Lalli E AUID- ORCID: 0000-0002-0584-5681 AD - Institut de Pharmacologie Moleculaire et Cellulaire CNRS UMR7275, Inserm U1423, Universite Cote d'Azur, 06560 Valbonne, France. FAU - Figueiredo, Bonald Cavalcante de AU - Figueiredo BC AD - Instituto de Pesquisa Pele Pequeno Principe, Curitiba 80250-060, PR, Brazil. AD - Faculdades Pequeno Principe, Reboucas, Curitiba 80230-020, PR, Brazil. LA - eng GR - 76.591.569/0001-30/2025/Behring Foundation/ PT - Journal Article PT - Review DEP - 20261018 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702201 OTO - NOTNLM OT - T-cell exhaustion OT - antigen escape OT - cellular fitness OT - immune evasion OT - predictive biomarkers OT - spatial transcriptomics OT - tumor microenvironment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/18 CRDT- 2026/10/27 10:32 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - cancers18203370 [pii] AID - cancers-18-03370 [pii] AID - 10.3390/cancers18203370 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 18;18(20):3370. doi: 10.3390/cancers18203370. PMID- 42938901 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 18 TI - Chloride Channels as Determinants of Anti-Cancer Drug Response: Why the Therapeutic Direction Reverses Between Channels and Between Tissues. LID - 10.3390/cancers18203369 [doi] LID - 3369 AB - Background/Objectives: Resistance to cytotoxic chemotherapy and radiotherapy is usually attributed to DNA repair, drug efflux and apoptotic threshold. Chloride channels intersect these processes but are rarely considered as a group. This review asks whether chloride flux forms a coherent, targetable axis of treatment response and, if not, how the therapeutic direction differs by channel and tissue. Methods: PubMed was searched to 23 August 2026; all free-text searches were re-executed on 22 September 2026 and supplemented by controlled-vocabulary searches. Cited records were checked against NCBI E-utilities and screened for retractions. Claims were graded by whether pharmacological phenotypes were reproduced by genetic perturbation and by the available target-engagement and replication evidence. Results: The therapeutic direction is not uniform. In experimental models, LRRC8A- and LRRC8D-containing channels mediate substantial platinum uptake, and dose-stratified associations between their expression and outcome have been reported in head and neck cancer, supporting prospective evaluation as predictive biomarkers; contrary reports in ovarian, colon and breast models indicate that this direction is established for platinum uptake rather than for cytotoxic resistance in general. CFTR is an intestinal tumour suppressor and the only channel reviewed with an approved potentiator, yet its direction reverses in glioma, with a prostate report of CFTR-dependent cisplatin resistance pointing in the same direction. Conversely, ClC-3, ANO1 and CLIC1 are associated with resistance when gained, although CLIC1 is better supported as a determinant of drug response than of tumorigenesis. Sixteen modulators are compared by potency, assay format, selectivity/counter-screening and clinical status; widely used blockers are often poorly selective. Conclusions: Chloride transport is not a single therapeutic target class. Candidate chloride-channel blockers should be counter-screened against LRRC8/VRAC before combination with platinum therapy, and CFTR potentiation should not be generalised across tumour types. Conflicting evidence is reported alongside the directions it qualifies rather than set aside. FAU - Seo, Yohan AU - Seo Y AUID- ORCID: 0000-0002-9335-8604 AD - Department of Physiology, Dongguk University College of Medicine, 123 Dongdae-ro, Gyeongju-si 38066, Gyeongsangbuk-do, Republic of Korea. LA - eng GR - K-2026-G0002-00007/Dongguk University WISE/ PT - Journal Article PT - Review DEP - 20261018 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702227 OTO - NOTNLM OT - ANO1/TMEM16A OT - CFTR OT - CLIC1 OT - ClC-3 OT - LRRC8 OT - VRAC OT - chloride channel OT - cisplatin OT - drug repurposing OT - drug resistance COIS- The author declares no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/18 CRDT- 2026/10/27 10:32 PHST- 2026/08/25 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - cancers18203369 [pii] AID - cancers-18-03369 [pii] AID - 10.3390/cancers18203369 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 18;18(20):3369. doi: 10.3390/cancers18203369. PMID- 42938899 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 18 TI - Giant Keratinocyte Carcinomas: Clinical, Histopathological, Molecular and Health-System Factors with Therapeutic Implications-A Structured Narrative Review. LID - 10.3390/cancers18203367 [doi] LID - 3367 AB - BACKGROUND/OBJECTIVES: Giant keratinocyte carcinomas, comprising basal cell carcinoma (BCC) and cutaneous squamous cell carcinoma (cSCC) with a maximum diameter >/= 5 cm, pose substantial clinical challenges. This structured narrative review distinguishes giant-specific findings from contextual evidence in advanced or high-risk disease. METHODS: The synthesis uses a retained bibliography assembled from searches described in earlier drafts as covering PubMed/MEDLINE and PubMed Central from January 2015 to March 2026, supplemented by citation and guideline searching and a focused update reported through 4 September 2026. A record-level search and screening log is unavailable; historical selection counts cannot be independently reconstructed. The review protocol was not registered, and exhaustive coverage or full PRISMA compliance is not claimed. Sources were classified by design and directness, with descriptive consideration of methodological limitations. RESULTS: The retained set contains 51 sources: nine containing direct giant-specific clinical evidence and 42 providing contextual evidence. Direct evidence consists principally of case reports, retrospective or comparative studies and a giant-cSCC literature synthesis. It links giant BCC with prolonged lesion duration, ulceration, deeper invasion and reconstructive burden. Giant-cSCC outcomes vary substantially between reported series. Evidence for Hedgehog-pathway inhibition, PD-1 blockade and most molecular mechanisms derives mainly from conventional or advanced disease. CONCLUSIONS: Management requires multidisciplinary assessment integrating size, pathological risk, resectability and patient factors. Giant-specific molecular and therapeutic evidence remains limited, and contextual treatment effects should not be interpreted as effects established specifically in tumours >/= 5 cm. FAU - Ciborek, Andrzej AU - Ciborek A AD - Department of Interprofessional Education, Faculty of Medicine and Health Sciences, Calisia University (Uniwersytet Kaliski im. Prezydenta Stanislawa Wojciechowskiego), ul. Kaszubska 13, 62-800 Kalisz, Poland. FAU - Krauss, Hanna AU - Krauss H AD - Department of Basic Sciences, Calisia University (Uniwersytet Kaliski im. Prezydenta Stanislawa Wojciechowskiego), ul. Kaszubska 13, 62-800 Kalisz, Poland. FAU - Checinska-Maciejewska, Zuzanna AU - Checinska-Maciejewska Z AUID- ORCID: 0000-0001-8884-3069 AD - Department of Food and Nutrition, Faculty of Medicine and Health Sciences, Calisia University, ul. Kaszubska 13, 62-800 Kalisz, Poland. FAU - Jaworek, Andrzej Kazimierz AU - Jaworek AK AUID- ORCID: 0000-0002-7779-2785 AD - Department of Dermatology, Jagiellonian University Medical College, ul. Mikolaja Kopernika 50, 31-501 Krakow, Poland. LA - eng PT - Journal Article PT - Review DEP - 20261018 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702539 OTO - NOTNLM OT - Hedgehog pathway OT - Mohs micrographic surgery OT - PD-1 blockade OT - cemiplimab OT - diagnostic delay OT - giant basal cell carcinoma OT - giant cutaneous squamous cell carcinoma OT - structured narrative review OT - tumour microenvironment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/18 CRDT- 2026/10/27 10:32 PHST- 2026/08/12 00:00 [received] PHST- 2026/09/14 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/18 00:00 [pmc-release] AID - cancers18203367 [pii] AID - cancers-18-03367 [pii] AID - 10.3390/cancers18203367 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 18;18(20):3367. doi: 10.3390/cancers18203367. PMID- 42938898 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 17 TI - Baseline Host Biomarkers and Six-Month Sustained Drug-Free Status After Atezolizumab-Bevacizumab for Hepatocellular Carcinoma: Exploratory Clinical Stratification Using PAN-DF Score. LID - 10.3390/cancers18203366 [doi] LID - 3366 AB - Background/Objectives: Sustained drug-free status is an emerging milestone after atezolizumab plus bevacizumab for unresectable hepatocellular carcinoma. We examined baseline host biomarkers associated with achievement of 6-month sustained drug-free status and evaluated a composite stratification score. Methods: This multicentre retrospective study included patients treated with atezolizumab plus bevacizumab. The endpoint required systemic therapy discontinuation after complete response or no viable disease, followed by at least 6 months without viable or recurrent hepatocellular carcinoma and sustained normalization of initially elevated tumour markers. The PAN-DF score (preserved hepatic reserve, altered immune-inflammatory status, and nutritional-immune reserve for achieving drug-free status) combined modified albumin-bilirubin grade, neutrophil-to-lymphocyte ratio, and prognostic nutritional index. Results: Among 655 patients, 25 achieved the endpoint; median post-discontinuation follow-up was 533 days. In the complete-case cohort (n = 473; 17 events), achievement rates were 0.0%, 2.9%, 3.7%, and 13.6% for scores 0-3. Each one-point increase was associated with higher odds of achievement (odds ratio 2.90, 95% confidence interval 1.51-5.55; p = 0.001), and the area under the curve was 0.719. The examined baseline tumour-related factors were not significantly associated with the primary endpoint, and the examined host-related factors were not associated with objective response. Conclusions: Selected baseline host biomarkers were associated with achievement of 6-month sustained drug-free status. PAN-DF stratified the observed probability of this outcome and may help identify patient groups with a relatively higher likelihood of a sustained drug-free state, supporting early multidisciplinary planning. FAU - Iino, Chikara AU - Iino C AD - Department of Gastroenterology, Hematology and Clinical Immunology, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Aomori, Japan. FAU - Abe, Tamami AU - Abe T AD - Department of Gastroenterology and Hepatology, Division of Internal Medicine, Iwate Medical University, Yahaba 028-3694, Iwate, Japan. FAU - Endo, Kei AU - Endo K AD - Department of Gastroenterology and Hepatology, Division of Internal Medicine, Iwate Medical University, Yahaba 028-3694, Iwate, Japan. FAU - Fujiwara, Yudai AU - Fujiwara Y AD - Department of Gastroenterology and Hepatology, Division of Internal Medicine, Iwate Medical University, Yahaba 028-3694, Iwate, Japan. FAU - Yoshida, Kenta AU - Yoshida K AUID- ORCID: 0000-0002-3897-5546 AD - Department of Gastroenterology, Hematology and Clinical Immunology, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Aomori, Japan. FAU - Sato, Satoshi AU - Sato S AUID- ORCID: 0000-0001-5569-7093 AD - Department of Gastroenterology, Hematology and Clinical Immunology, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Aomori, Japan. FAU - Numao, Hiroshi AU - Numao H AD - Department of Gastroenterology, Aomori Prefectural Central Hospital, Aomori 030-8553, Aomori, Japan. FAU - Igarashi, Go AU - Igarashi G AD - Department of Gastroenterology, Aomori Prefectural Central Hospital, Aomori 030-8553, Aomori, Japan. FAU - Oikawa, Takayoshi AU - Oikawa T AUID- ORCID: 0000-0002-1100-6980 AD - Department of Gastroenterology, Japanese Red Cross Morioka Hospital, Morioka 020-8560, Iwate, Japan. FAU - Ninomiya, Masashi AU - Ninomiya M AD - Division of Gastroenterology, Tohoku University Hospital, Sendai 980-8574, Miyagi, Japan. FAU - Tsuruoka, Mio AU - Tsuruoka M AD - Division of Gastroenterology, Tohoku University Hospital, Sendai 980-8574, Miyagi, Japan. FAU - Sato, Wataru AU - Sato W AD - Department of Gastroenterology, Akita University Graduate School of Medicine, Akita 010-8543, Akita, Japan. FAU - Minami, Shinichiro AU - Minami S AUID- ORCID: 0009-0008-8735-291X AD - Department of Gastroenterology, Akita University Graduate School of Medicine, Akita 010-8543, Akita, Japan. FAU - Abe, Kazumichi AU - Abe K AUID- ORCID: 0000-0001-5359-9465 AD - Department of Gastroenterology, Fukushima Medical University School of Medicine, Fukushima 960-1295, Fukushima, Japan. FAU - Fujita, Masashi AU - Fujita M AD - Department of Gastroenterology, Fukushima Medical University School of Medicine, Fukushima 960-1295, Fukushima, Japan. FAU - Tanabe, Nobukazu AU - Tanabe N AD - Department of Gastroenterology, National Hospital Organization Sendai Medical Center, Sendai 983-8520, Miyagi, Japan. FAU - Katsumi, Tomohiro AU - Katsumi T AUID- ORCID: 0000-0001-5670-7505 AD - Department of Gastroenterology, Yamagata University Faculty of Medicine, Yamagata 990-9585, Yamagata, Japan. FAU - Maki, Keita AU - Maki K AUID- ORCID: 0009-0003-2035-1940 AD - Department of Gastroenterology, Yamagata University Faculty of Medicine, Yamagata 990-9585, Yamagata, Japan. FAU - Suda, Goki AU - Suda G AD - Department of Gastroenterology and Hepatology, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Hokkaido, Japan. FAU - Sho, Takuya AU - Sho T AUID- ORCID: 0000-0001-7943-3842 AD - Department of Gastroenterology and Hepatology, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Hokkaido, Japan. FAU - Yamamoto, Yoshiya AU - Yamamoto Y AD - Department of Gastroenterology, Hakodate City Hospital, Hakodate 041-8680, Hokkaido, Japan. FAU - Baba, Masaru AU - Baba M AUID- ORCID: 0000-0002-4142-3267 AD - Department of Gastroenterology and Hepatology, Japan Community Health Care Organization (JCHO) Hokkaido Hospital, Sapporo 062-8618, Hokkaido, Japan. FAU - Meguro, Takashi AU - Meguro T AD - Department of Gastroenterology, Hokkaido Gastroenterology Hospital, Sapporo 065-0041, Hokkaido, Japan. FAU - Sakuraba, Hirotake AU - Sakuraba H AUID- ORCID: 0000-0002-6075-1282 AD - Department of Gastroenterology, Hematology and Clinical Immunology, Hirosaki University Graduate School of Medicine, Hirosaki 036-8562, Aomori, Japan. FAU - Sakamoto, Naoya AU - Sakamoto N AUID- ORCID: 0000-0003-0061-059X AD - Department of Gastroenterology and Hepatology, Graduate School of Medicine, Hokkaido University, Sapporo 060-8638, Hokkaido, Japan. FAU - Ueno, Yoshiyuki AU - Ueno Y AUID- ORCID: 0000-0001-5623-4250 AD - Department of Gastroenterology, Yamagata University Faculty of Medicine, Yamagata 990-9585, Yamagata, Japan. FAU - Kuroda, Hidekatsu AU - Kuroda H AUID- ORCID: 0000-0002-1471-1087 AD - Department of Gastroenterology and Hepatology, Division of Internal Medicine, Iwate Medical University, Yahaba 028-3694, Iwate, Japan. CN - NORTH Japan HCC Study Group LA - eng PT - Journal Article DEP - 20261017 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702456 OTO - NOTNLM OT - atezolizumab OT - bevacizumab OT - clinical stratification OT - conversion therapy OT - drug-free status OT - hepatocellular carcinoma OT - host biomarkers COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/17 CRDT- 2026/10/27 10:32 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/15 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - cancers18203366 [pii] AID - cancers-18-03366 [pii] AID - 10.3390/cancers18203366 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 17;18(20):3366. doi: 10.3390/cancers18203366. PMID- 42938896 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 17 TI - Molecular Reclassification of Diffuse Gliomas: A Feasibility Study of Clinical Features and Survival Outcomes in Colombia. LID - 10.3390/cancers18203364 [doi] LID - 3364 AB - Background/Objectives: The 2021 World Health Organization Classification of Tumors of the Central Nervous System (WHO CNS5) integrates histological and molecular criteria for adult diffuse gliomas, but comprehensive molecular testing may be difficult to implement in resource-limited settings. This study assessed the feasibility of a sequential, marker-based approach for diagnostic reassessment and described its association with clinical characteristics and overall survival (OS) in adults from Colombia. Methods: We retrospectively analyzed 68 adults with diffuse gliomas treated at two referral hospitals in Bogota, Colombia, between 2015 and 2023. Molecular assessment used IHC, FISH, PCR, and Sanger sequencing according to the available tissue and diagnostic workflow. The study did not include comprehensive NGS or systematic assessment of combined whole-chromosome 7 gain/chromosome 10 loss (+7/-10). Results: The diagnosis changed after molecular reassessment in 51.5% of cases. The proportion classified as glioblastoma increased from 48.5% under the historical histologic classification to 66.2% under the WHO CNS5-based algorithm, whereas FISH-defined oligodendrogliomas decreased from 17.6% to 11.8%. OS differed among historical histologic groups (p = 0.03), but not among the WHO CNS5-based groups (p = 0.13). Female sex was associated with longer OS in unadjusted analysis (p = 0.02), while age was the only independent predictor in the multivariable analysis. Conclusions: Sequential molecular reassessment materially changed diagnostic categorization in this cohort and was feasible using locally available marker-based methods. However, the results are exploratory, limited by small molecular subgroups, incomplete molecular coverage, and the retrospective treatment context; they should not be interpreted as demonstrating improved treatment selection or survival after reclassification. FAU - Ordonez-Rubiano, Edgar G AU - Ordonez-Rubiano EG AUID- ORCID: 0000-0003-1179-5825 AD - School of Medicine, Universidad Nacional de Colombia, Bogota 111321, Colombia. AD - Department of Neurosurgery, Universidad FUCS, Hospital de San Jose-Sociedad de Cirugia de Bogota, Bogota 111411, Colombia. AD - Department of Neurosurgery, Hospital Universitario Fundacion Santa Fe de Bogota, Bogota 110111, Colombia. FAU - Rodriguez, Fabiana AU - Rodriguez F AD - Department of Pathology, Universidad FUCS, Hospital de San Jose-Sociedad de Cirugia de Bogota, Bogota 111411, Colombia. FAU - Siabato-Vargas, Cristian F AU - Siabato-Vargas CF AD - Group of Basic Sciences in Health, Universidad FUCS, Hospital Infantil Universitario de San Jose, Bogota 111221, Colombia. FAU - Gutierrez-Castaneda, Luz Dary AU - Gutierrez-Castaneda LD AD - Group of Basic Sciences in Health, Universidad FUCS, Hospital Infantil Universitario de San Jose, Bogota 111221, Colombia. FAU - Rincon-Arias, Nicolas AU - Rincon-Arias N AUID- ORCID: 0009-0007-4081-5924 AD - Department of Neurosurgery, Universidad FUCS, Hospital de San Jose-Sociedad de Cirugia de Bogota, Bogota 111411, Colombia. FAU - Espinosa, Sebastian AU - Espinosa S AD - Department of Neurosurgery, Universidad FUCS, Hospital de San Jose-Sociedad de Cirugia de Bogota, Bogota 111411, Colombia. FAU - Combita, Alba AU - Combita A AD - School of Medicine, Universidad Nacional de Colombia, Bogota 111321, Colombia. AD - Department of Microbiology, Universidad Nacional de Colombia, Bogota 111321, Colombia. FAU - Baldoncini, Matias AU - Baldoncini M AUID- ORCID: 0000-0001-9323-8306 AD - Department of Neurosurgery, San Fernando Hospital, San Fernando B1646, Buenos Aires, Argentina. FAU - Gomez-Amarillo, Diego F AU - Gomez-Amarillo DF AD - Department of Neurosurgery, Hospital Universitario Fundacion Santa Fe de Bogota, Bogota 110111, Colombia. FAU - Hakim, Fernando AU - Hakim F AD - Department of Neurosurgery, Hospital Universitario Fundacion Santa Fe de Bogota, Bogota 110111, Colombia. FAU - Andrade-Andrade, Pedro AU - Andrade-Andrade P AUID- ORCID: 0009-0005-5872-3580 AD - Department of Neurosurgery, Hospital Universitario Fundacion Santa Fe de Bogota, Bogota 110111, Colombia. AD - School of Medicine, Universidad de Los Andes, Bogota 111711, Colombia. FAU - Payan-Gomez, Cesar AU - Payan-Gomez C AUID- ORCID: 0000-0002-0633-1332 AD - Direccion Academica, Universidad Nacional de Colombia, Sede de La Paz, La Paz 202010, Colombia. FAU - Zorro-Guio, Oscar F AU - Zorro-Guio OF AD - Department of Neurosurgery, Universidad FUCS, Hospital de San Jose-Sociedad de Cirugia de Bogota, Bogota 111411, Colombia. FAU - Patino-Gomez, Javier G AU - Patino-Gomez JG AD - Department of Neurosurgery, Universidad FUCS, Hospital de San Jose-Sociedad de Cirugia de Bogota, Bogota 111411, Colombia. FAU - Riveros-Barrera, Irene AU - Riveros-Barrera I AD - Department of Pathology, Instituto Nacional de Cancerologia, Bogota 111511, Colombia. FAU - Mendoza, Oscar AU - Mendoza O AD - Department of Pathology, Universidad FUCS, Hospital de San Jose-Sociedad de Cirugia de Bogota, Bogota 111411, Colombia. FAU - Parra-Medina, Rafael AU - Parra-Medina R AUID- ORCID: 0000-0002-8428-5837 AD - Department of Pathology, Instituto Nacional de Cancerologia, Bogota 111511, Colombia. AD - Research Institute, Universidad FUCS, Hospital de San Jose-Sociedad de Cirugia de Bogota, Bogota 111411, Colombia. LA - eng GR - (Convocatoria Fomento a la Investigacion) under grant number 1-2023, session of February 10, 2023./Universidad FUCS/ PT - Journal Article DEP - 20261017 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702763 OTO - NOTNLM OT - World Health Organization OT - astrocytoma OT - classification OT - glioblastoma OT - glioma OT - oligodendroglioma COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/17 CRDT- 2026/10/27 10:32 PHST- 2026/08/26 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - cancers18203364 [pii] AID - cancers-18-03364 [pii] AID - 10.3390/cancers18203364 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 17;18(20):3364. doi: 10.3390/cancers18203364. PMID- 42938894 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 17 TI - Convolutional, State-Space and Transformer Liver-Tumor Segmentation Models Trained on Hepatocellular Carcinoma Fail on the Same External Cases: Lesion Size Outweighs Architecture in Cross-Dataset Generalization. LID - 10.3390/cancers18203362 [doi] LID - 3362 AB - BACKGROUND/OBJECTIVES: Architectural innovation is widely assumed to improve the cross-institutional generalization of tumor segmentation models, yet this is rarely tested. We compared three architectural families under an identical, leak-safe protocol in hepatocellular carcinoma (HCC) segmentation. METHODS: Convolutional (nnU-Net ResEnc-M), state-space (U-Mamba Bot) and transformer (SwinUNETR) models were trained on HCC-TACE-Seg (n = 103) with identical patient-disjoint five-fold splits, then applied without adaptation to 3D-IRCADb (n = 15) and LiTS (n = 118). We assessed discrimination, case-level failure concordance, lesion-volume dependence, calibration and selective prediction. RESULTS: Internal performance differed (Dice 0.685, 0.662, 0.479; Friedman p = 0.022) but external performance was low and largely convergent: the convolutional and state-space models were formally equivalent on both external cohorts (pre-specified margin +/-0.05 Dice), whereas the transformer was inferior on LiTS (0.222; p < 0.0001) and inconclusive on 3D-IRCADb. Case-level Dice was highly concordant across all pairings (Spearman rho = 0.84-0.96; 0.67-0.81 on LiTS after adjustment for lesion volume), the same cases failing across architectures. Lesion volume predicted performance in all six architecture x cohort combinations; lesions < 5 mL failed completely in 61-76% of cases and formed 1.9% of the development cohort against 31.4% of LiTS. Neither liver-mask constraint nor ensembling recovered performance. Deferring the 30% least-confident LiTS cases raised Dice to 0.516 (0.450 when only empty predictions were removed). CONCLUSIONS: Under a single-source, TACE-treated HCC training regime, cross-dataset failure of liver-tumor segmentation was governed primarily by lesion size rather than by architectural family; confidence-based selective prediction offers a deployable mitigation. FAU - Kutlu, Huseyin AU - Kutlu H AUID- ORCID: 0000-0003-0091-9984 AD - Department of Biostatistics and Medical Informatics, Faculty of Medicine, Adiyaman University, 02040 Adiyaman, Turkiye. AD - Department of Biostatistics and Medical Informatics, Faculty of Medicine, Inonu University, 44280 Malatya, Turkiye. FAU - Colak, Cemil AU - Colak C AUID- ORCID: 0000-0001-5406-098X AD - Department of Biostatistics and Medical Informatics, Faculty of Medicine, Inonu University, 44280 Malatya, Turkiye. FAU - Akbulut, Sami AU - Akbulut S AUID- ORCID: 0000-0002-6864-7711 AD - Department of Biostatistics and Medical Informatics, Faculty of Medicine, Inonu University, 44280 Malatya, Turkiye. AD - Department of Surgery and Liver Transplantation, Faculty of Medicine, Inonu University, 44280 Malatya, Turkiye. LA - eng GR - Inonu University, Scientific Research Projects Coordination Unit (Bilimsel Arastirma Projeleri Koordinasyon Birimi) grant number TSA-2026-5224/Inonu University/ PT - Journal Article DEP - 20261017 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702596 OTO - NOTNLM OT - computed tomography OT - deep learning OT - domain shift OT - external validation OT - hepatocellular carcinoma OT - lesion size OT - liver-tumor segmentation OT - model calibration OT - selective prediction COIS- The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analysis, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/17 CRDT- 2026/10/27 10:32 PHST- 2026/09/14 00:00 [received] PHST- 2026/10/02 00:00 [revised] PHST- 2026/10/05 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - cancers18203362 [pii] AID - cancers-18-03362 [pii] AID - 10.3390/cancers18203362 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 17;18(20):3362. doi: 10.3390/cancers18203362. PMID- 42938893 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 17 TI - Selection Instability and External Performance of Transcriptomic Models Beyond Age, Sex and Stage in Resected Lung Adenocarcinoma. LID - 10.3390/cancers18203361 [doi] LID - 3361 AB - BACKGROUND/OBJECTIVES: We evaluated molecular selection stability and external prediction beyond age, sex and broad pathological stage in resected stage I-II lung adenocarcinoma. METHODS: Models were developed in 228 patients from two sources and frozen before evaluation in four historical cohorts containing 778 patients. The primary comparison added pathway scores using elastic-net Cox regression. Secondary analyses examined a gene model, 200 development bootstrap resamples, alternative modeling specifications and locally updated baselines. Prediction error was measured by the integrated Brier score over 1-3 years (IBS); negative molecular-minus-clinical differences (DeltaIBS) favored molecular models. Evaluation intervals used 2000 paired bootstrap resamples. RESULTS: The pathway model retained no molecular terms. The final gene model retained 16 genes, whereas both source-held-out fits and 159/200 development resamples retained none. Penalties of 0.5, 0.15 and 0.05 were selected in 160, 38 and 2 resamples. Alternative specifications changed gene retention from 0 to 61 and pathway retention from 0 to 5. Frozen gene-model DeltaIBS ranged from -0.0034 to 0.0055 across cohorts. Its equal cohort mean was 0.0016 (95% CI, -0.0012 to 0.0042), compared with -0.0008 (-0.0024 to 0.0007) after cross-fitted local baseline updating. The clinical model predicted a 3-year mortality of 19.6% in Japan and 22.6% in Moffitt, versus observed values of 9.1% and 28.6%. CONCLUSIONS: Molecular selection was unstable, and incremental performance depended on specification and population. The results characterize these learning procedures and their limited development sample; broader transcriptomic prognostic value and contemporary clinical utility remain unestablished. FAU - Lei, Zhihao AU - Lei Z AD - Department of Statistics and Data Science, Cornell University, Ithaca, NY 14853, USA. FAU - Zou, Chenxi AU - Zou C AUID- ORCID: 0009-0005-0481-3863 AD - Department of Pulmonary and Critical Care Medicine, The First Medical Center, Chinese PLA General Hospital, Beijing 100853, China. FAU - Zhao, Wei AU - Zhao W AD - Senior Department of Pulmonary and Critical Care Medicine, The Eighth Medical Center of Chinese PLA General Hospital, Beijing 100091, China. LA - eng GR - 2024ZD0529503/National Health Commission of the People's Republic of China/ PT - Journal Article DEP - 20261017 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702175 OTO - NOTNLM OT - calibration OT - external validation OT - lung adenocarcinoma OT - machine learning OT - survival analysis OT - transcriptomics OT - transportability COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/17 CRDT- 2026/10/27 10:32 PHST- 2026/09/16 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - cancers18203361 [pii] AID - cancers-18-03361 [pii] AID - 10.3390/cancers18203361 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 17;18(20):3361. doi: 10.3390/cancers18203361. PMID- 42938891 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 17 TI - Prediction Models for Pathological Response to Neoadjuvant Chemoradiotherapy in Locally Advanced Rectal Cancer: A Systematic Review and Meta-Analysis. LID - 10.3390/cancers18203359 [doi] LID - 3359 AB - Background: Accurate pretreatment prediction of pCR is critical for stratifying patients with LARC and selecting candidates for organ-preserving approaches. However, pCR is a postoperative pathological endpoint, whereas watch-and-wait strategies are based on cCR. Despite a rapidly expanding literature encompassing gene expression signatures, MRI radiomics, deep learning, and multi-modal fusion models, no prior systematic review has comprehensively evaluated predictive performance across all modalities using contemporary meta-analytic techniques for diagnostic test accuracy. Methods: Systematic review and random- effects meta-analysis of studies developing or validating predictive models for pCR in LARC treated with neoadjuvant chemoradiotherapy (nCRT) were conducted. Summary AUC was estimated using logit-transformed random-effects models. Heterogeneity was quantified by I(2) and tau(2). Subgroup analyses were performed according to model modality and validation strategy. Publication bias was assessed with Egger's linear regression test. Sensitivity analyses were performed using leave-one-out cross-validation. Results: Forty-three studies with 67,302 patients were included. The pooled AUC across all models was 0.874 (95% CI: 0.855-0.892), with substantial heterogeneity (I(2) = 94.3%, tau(2) = 0.247). Deep learning models showed the highest pooled AUC (0.919, 95% CI: 0.877-0.948), with MRI radiomics (0.885, 95% CI: 0.852-0.911), multi-modal (0.875, 95% CI: 0.813-0.919), gene expression (0.855, 95% CI: 0.797-0.898), and clinical factors (0.820, 95% CI: 0.788-0.849) showing progressively lower pooled estimates. External validation cohorts had a lower pooled AUC (0.864) than internal validation (0.884). Significant publication bias was detected (Egger's test p < 0.001). Leave-one-out sensitivity analysis confirmed robustness of the overall estimate. Conclusions: Deep learning and MRI radiomics models demonstrate promising diagnostic accuracy for predicting pCR after nCRT in LARC. However, the predominance of retrospective, single-center designs with limited external validation represents a critical translational gap, and the lack of studies in the total neoadjuvant therapy era limits generalizability. Prospective, multicenter validation studies incorporating standardized endpoints and class imbalance-appropriate metrics are urgently needed. FAU - Luo, Yun AU - Luo Y AUID- ORCID: 0009-0004-8394-4505 AD - Medical Big Data and Bioinformatics Research Center, First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China. AD - The First School of Clinical Medicine, Gannan Medical University, Ganzhou 341000, China. AD - Ganzhou Key Laboratory of Medical Big Data, Ganzhou 341000, China. AD - Gannan Subcenter for Medical Genomics, National Genomics Data Center, Ganzhou 341000, China. FAU - Ding, Youping AU - Ding Y AD - Medical Big Data and Bioinformatics Research Center, First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China. AD - The First School of Clinical Medicine, Gannan Medical University, Ganzhou 341000, China. AD - Ganzhou Key Laboratory of Medical Big Data, Ganzhou 341000, China. AD - Gannan Subcenter for Medical Genomics, National Genomics Data Center, Ganzhou 341000, China. FAU - Liu, Wei AU - Liu W AD - Medical Big Data and Bioinformatics Research Center, First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China. AD - The First School of Clinical Medicine, Gannan Medical University, Ganzhou 341000, China. AD - Ganzhou Key Laboratory of Medical Big Data, Ganzhou 341000, China. AD - Gannan Subcenter for Medical Genomics, National Genomics Data Center, Ganzhou 341000, China. FAU - Liu, Mengying AU - Liu M AD - Medical Big Data and Bioinformatics Research Center, First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China. AD - The First School of Clinical Medicine, Gannan Medical University, Ganzhou 341000, China. AD - Ganzhou Key Laboratory of Medical Big Data, Ganzhou 341000, China. AD - Gannan Subcenter for Medical Genomics, National Genomics Data Center, Ganzhou 341000, China. FAU - Zhao, Qiming AU - Zhao Q AD - Medical Big Data and Bioinformatics Research Center, First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China. AD - School of Public Health and Health Management, Gannan Medical University, Ganzhou 341000, China. FAU - Ouyang, Minglian AU - Ouyang M AD - The First School of Clinical Medicine, Gannan Medical University, Ganzhou 341000, China. FAU - Guo, You AU - Guo Y AUID- ORCID: 0000-0002-2751-3899 AD - Medical Big Data and Bioinformatics Research Center, First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China. AD - The First School of Clinical Medicine, Gannan Medical University, Ganzhou 341000, China. AD - Ganzhou Key Laboratory of Medical Big Data, Ganzhou 341000, China. AD - Gannan Subcenter for Medical Genomics, National Genomics Data Center, Ganzhou 341000, China. LA - eng GR - 82060618/National Natural Science Foundation of China/ PT - Journal Article PT - Review DEP - 20261017 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702563 OTO - NOTNLM OT - deep learning OT - locally advanced rectal cancer OT - machine learning OT - meta-analysis OT - prediction model COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/17 CRDT- 2026/10/27 10:32 PHST- 2026/09/29 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/17 00:00 [pmc-release] AID - cancers18203359 [pii] AID - cancers-18-03359 [pii] AID - 10.3390/cancers18203359 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 17;18(20):3359. doi: 10.3390/cancers18203359. PMID- 42938886 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 16 TI - Sex Hormone Estrone Sulfate Serendipitously Downregulates Intercellular Tunneling Nanotube Conduits, EMT, and Migration in Breast and Pancreatic Cancer Cells. LID - 10.3390/cancers18203354 [doi] LID - 3354 AB - Aim: Estrone (E1) is of particular interest as most breast cancers are diagnosed after menopause, the period in which E1 becomes the dominant estrogen. Triple-negative breast cancer (TNBC) is a highly invasive cancer that lacks hormone receptors for estrogen (ER), progesterone (PR), and HER2 (human epidermal growth factor receptor-2). TNBC management remains clinically challenging despite recent advances in targeted and immunotherapeutic approaches. This study investigated the effects of the estrogen hormone estrone sulfate (E1S) on cancer cells. Here, E1S engages in non-classical biased G protein-coupled estrogen receptor (GPER) signaling in TNBC MDA-MB-231 cells, estrogen receptor-positive MCF-7 breast cancer cells, and pancreatic PANC-1 cancer cells, which express estrogen, progesterone, and androgen receptors. Methods: We performed sialidase and colocalization assays in breast MDA-MB-231 and MCF-7 cells and pancreatic PANC-1 cancer cells. An epithelial-to-mesenchymal (EMT) transition, a tunneling nanotube assay, and a scratch wound assay were conducted to elucidate the cellular behaviors of E1S. We used a RawBlue reporter macrophage cell line to measure NFkappaB activity using the secreted embryonic alkaline phosphatase (SEAP) assay, and we measured cellular viability and metabolic activity using the AlamarBlue assay. Results: E1S induced NMBR/MMP9/Neu1 signaling in MDA-MB-231, MCF-7, PANC-1 and RawBlue cells. E1S increased E-cadherin expression, reduced N-cadherin and vimentin expression, decreased tunneling nanotube density, and reduced scratch wound migration. E1S increased SEAP activity, suggestive of increased nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1)-mediated transcription. E1S treatment did not affect cell viability and metabolic activity in triple-negative MDA-MB-231 breast cells using the AlamarBlue assay in a dose-dependent manner. Conclusions: E1S binding to GPER potentiates a biased heterodimerization with the GPCR neuromedin B receptor (NMBR) to activate matrix metalloproteinase 9 (MMP9) and neuraminidase 1 (Neu1), a signaling paradigm with previously established receptor tyrosine kinase (RTK) and Toll-like (TLR) receptors. Biased, temporal GPER signaling has significant implications for breast and pancreatic cancer cellular behavior, which may be leveraged to inform therapeutic strategies for aggressive forms of breast and pancreatic cancers. This research study is mechanistic in nature and may have potential translational implications. FAU - Wahab, Izzah AU - Wahab I AD - Department of Biomedical & Molecular Sciences, Queen's University, Kingston, ON K7L 3N6, Canada. FAU - Li, Yunfan AU - Li Y AD - Department of Biomedical & Molecular Sciences, Queen's University, Kingston, ON K7L 3N6, Canada. FAU - Szewczuk, Myron R AU - Szewczuk MR AUID- ORCID: 0000-0001-8471-5481 AD - Department of Biomedical & Molecular Sciences, Queen's University, Kingston, ON K7L 3N6, Canada. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702676 OTO - NOTNLM OT - MDA-MB-231, MCF-7, PANC-1 and RawBlue cells OT - a tunneling nanotube assay OT - epithelial-to-mesenchymal (EMT) transition OT - estrone OT - scratch wound COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/16 CRDT- 2026/10/27 10:32 PHST- 2026/09/05 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - cancers18203354 [pii] AID - cancers-18-03354 [pii] AID - 10.3390/cancers18203354 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 16;18(20):3354. doi: 10.3390/cancers18203354. PMID- 42938885 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 16 TI - A Leakage-Controlled Benchmark of Metaheuristic Multi-Omics Feature Selection for Reconstructing TCGA Molecular Subtypes in Endometrial Cancer. LID - 10.3390/cancers18203353 [doi] LID - 3353 AB - Background: The four molecular subtypes of endometrial carcinoma defined by The Cancer Genome Atlas (TCGA) can be reconstructed from multi-omics data, but these data contain about 460,000 features for 369 patients, the subtypes are imbalanced, and feature selection easily leaks test-set information. Methods: We developed POISE-X, a leakage-controlled, imbalance-aware benchmarking pipeline with an interchangeable metaheuristic search engine. Using RNA-seq, miRNA, DNA methylation, copy-number, mutation, and clinical data from 369 TCGA-UCEC patients, we compared 19 optimizers under nested five-fold cross-validation, with in-fold prefiltering to 2505 candidate features and a macro-averaged F1 fitness function. Results: Performance differed across optimizers in a Friedman test on fold means (p=1.989x10-6). No selected panel exceeded logistic regression on the full candidate pool, which reached a macro-averaged F1 of 0.902; the compact configuration chosen by our selection rule, POISE-GWO, retained 443 features with a macro-averaged F1 of 0.880, and the selection rule itself scored 0.881 under leave-one-fold-out evaluation, and an ANOVA-ranked panel of 450 features reached 0.886. These results held without clinical variables and, in the two searches tested, for parsimony weights of 0.8-0.99. A surrogate of the clinical molecular classification built from POLE exonuclease-domain mutation, mismatch-repair deficiency, and TP53 mutation reached a macro-averaged F1 of 0.924, higher on average than every multi-omics model. Conclusions: In this cohort, wrapper search therefore compressed the feature set without adding discriminative power, and the selected panels reconstructed an existing classification, not a new treatment-relevant signature. POISE-X is a framework for benchmarking feature-selection strategies; any selected panel requires external validation before clinical use. FAU - Cao, Minyue AU - Cao M AD - Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China. FAU - Zhang, Yiqin AU - Zhang Y AD - Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China. FAU - Ding, Yan AU - Ding Y AD - Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China. FAU - Jiang, Genyi AU - Jiang G AUID- ORCID: 0000-0003-1548-5533 AD - Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China. FAU - Yan, Jiayu AU - Yan J AD - Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China. FAU - Sun, Yihan AU - Sun Y AD - Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China. FAU - Zhang, Yanli AU - Zhang Y AUID- ORCID: 0000-0002-8265-3499 AD - Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China. FAU - Kang, Luyao AU - Kang L AD - Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China. FAU - Luo, Jing AU - Luo J AD - Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China. FAU - Li, Bilan AU - Li B AD - Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China. LA - eng GR - 82372925/National Natural Science Foundation of China/ GR - 82172714/National Natural Science Foundation of China/ GR - 82674378/National Natural Science Foundation of China/ GR - 22Y11906300/Natural Science Foundation of Shanghai/ GR - 20ZR1443900/Natural Science Foundation of Shanghai/ GR - SHDC2020CR4086/Clinical Research Plan of SHDC/ GR - QNWS2024042/Shanghai Oriental Talents Program/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702640 OTO - NOTNLM OT - endometrial carcinoma OT - feature selection OT - metaheuristic optimization OT - molecular subtyping OT - multi-omics data integration COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/16 CRDT- 2026/10/27 10:32 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - cancers18203353 [pii] AID - cancers-18-03353 [pii] AID - 10.3390/cancers18203353 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 16;18(20):3353. doi: 10.3390/cancers18203353. PMID- 42938883 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 16 TI - Evaluation of the Diagnostic and Prognostic Potential, and Correlation with the Tumor Immune Microenvironment of a Six-miRNA Signature Associated with Platinum Sensitivity in Patients with Early-Stage Non-Small-Cell Lung Cancer. LID - 10.3390/cancers18203351 [doi] LID - 3351 AB - BACKGROUND/OBJECTIVES: Platinum-based chemotherapy remains a standard-of-care treatment for non-small-cell lung cancer (NSCLC), yet its efficacy is influenced by multiple factors, such as the functionality of DNA damage response pathways. DNA-damaging agents, like cisplatin, contribute to the generation of an "inflamed" tumor microenvironment (TME), which in turn correlates with an active antitumor immune response. MicroRNAs (miRNAs), a family of small, non-coding RNAs, have emerged as critical post-transcriptional regulators of gene expression, playing vital roles in modulating DNA Damage Repair (DDR) pathways and sensitivity to cisplatin. Their functional involvement in these mechanisms underscores their potential as diagnostic and prognostic biomarkers in lung cancer. METHODS: In this study, we investigated the diagnostic and prognostic value of a previously validated six-miRNA signature (hsa-miR-26a, hsa-miR-29c, hsa-miR-34a, hsa-miR-30-5p, hsa-miR-30e-3p and hsa-miR-497) in paired tumor and healthy tissue samples from surgically treated NSCLC patients (n = 76), who later received platinum-based therapy. To explore the translational applicability of this signature, we also compared miRNA expression levels between matched tumor tissue and plasma samples (n = 36). We further examined the presence of an "inflamed" TME and its association with the miRNA signature by examining the mRNA levels of TIGIT, CD8A, FOXP3, CXCL10 and PD-L1, and performing correlation analysis. RESULTS: All selected miRNAs were significantly downregulated in NSCLC tumor tissue compared to healthy samples. Moreover, miRNA expression was differentially regulated in plasma samples, supporting their potential clinical relevance as minimally invasive biomarkers. The complete six-miRNA signature demonstrated good discriminatory ability between tumor and matched non-malignant tissue (AUC = 0.786, 95% CI: 0.713-0.860; p < 0.001), with 72% sensitivity and 77.3% specificity. A significant correlation with relapse-free survival (RFS) (p = 0.039) was also observed. Distinct immune-related transcriptional alterations were observed in tumor tissue, including increased expression of immune checkpoint-associated genes (TIGIT and PD-L1) and reduced CD8A expression. Tumor PD-L1 expression showed modest positive correlations with miR-26a (p = 0.046) and miR-30e-3p (p = 0.031). CONCLUSIONS: Our findings underscore the potential value of this novel six-miRNA signature both as a prognostic and diagnostic biomarker in NSCLC. Its correlation with immune microenvironment characteristics supports its relevance in guiding personalized therapeutic strategies. This signature warrants further clinical validation and may serve as a valuable tool for patient stratification and outcome prediction for patients treated with platinum-based chemotherapy. FAU - Mortoglou, Maria AU - Mortoglou M AUID- ORCID: 0000-0003-4394-4549 AD - Laboratory of Translational Oncology, School of Medicine, University of Crete, 71500 Heraklion, Greece. FAU - Papadaki, Chara AU - Papadaki C AUID- ORCID: 0000-0002-4591-3757 AD - Laboratory of Translational Oncology, School of Medicine, University of Crete, 71500 Heraklion, Greece. FAU - Boukouris, Aristeidis E AU - Boukouris AE AUID- ORCID: 0000-0002-7106-0356 AD - Laboratory of Translational Oncology, School of Medicine, University of Crete, 71500 Heraklion, Greece. AD - Department of Medical Oncology, University General Hospital of Heraklion, 71500 Heraklion, Greece. FAU - Michaelidou, Kleita AU - Michaelidou K AUID- ORCID: 0000-0002-4425-7145 AD - Laboratory of Translational Oncology, School of Medicine, University of Crete, 71500 Heraklion, Greece. FAU - Vorrias, Eleftherios AU - Vorrias E AD - Laboratory of Translational Oncology, School of Medicine, University of Crete, 71500 Heraklion, Greece. AD - Department of Medical Oncology, University General Hospital of Heraklion, 71500 Heraklion, Greece. FAU - Kamaratou, Maria AU - Kamaratou M AD - Laboratory of Translational Oncology, School of Medicine, University of Crete, 71500 Heraklion, Greece. AD - Department of Medical Oncology, University General Hospital of Heraklion, 71500 Heraklion, Greece. FAU - Koronakis, Georgios AU - Koronakis G AD - Laboratory of Translational Oncology, School of Medicine, University of Crete, 71500 Heraklion, Greece. AD - Department of Medical Oncology, University General Hospital of Heraklion, 71500 Heraklion, Greece. FAU - Lagoudaki, Eleni AU - Lagoudaki E AD - Department of Pathology, University General Hospital of Heraklion, 71500 Heraklion, Greece. AD - School of Medicine, University of Crete, 70013 Heraklion, Greece. FAU - Koutsopoulos, Anastasios AU - Koutsopoulos A AUID- ORCID: 0000-0002-8430-7565 AD - Department of Pathology, University General Hospital of Heraklion, 71500 Heraklion, Greece. AD - School of Medicine, University of Crete, 70013 Heraklion, Greece. FAU - Mavroudis, Dimitrios AU - Mavroudis D AUID- ORCID: 0000-0003-0587-1713 AD - Laboratory of Translational Oncology, School of Medicine, University of Crete, 71500 Heraklion, Greece. AD - Department of Medical Oncology, University General Hospital of Heraklion, 71500 Heraklion, Greece. FAU - Agelaki, Sofia AU - Agelaki S AD - Laboratory of Translational Oncology, School of Medicine, University of Crete, 71500 Heraklion, Greece. AD - Department of Medical Oncology, University General Hospital of Heraklion, 71500 Heraklion, Greece. LA - eng GR - Anticancer Research Support Association (ARSA)/ GR - Hellenic Society of Medical Oncology (HeSMO)/ PT - Journal Article DEP - 20261016 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702743 OTO - NOTNLM OT - NSCLC OT - chemotherapy OT - microRNAs OT - non-coding RNAs OT - platinum-based chemotherapy OT - therapeutic resistance COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/16 CRDT- 2026/10/27 10:32 PHST- 2026/09/18 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:32 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - cancers18203351 [pii] AID - cancers-18-03351 [pii] AID - 10.3390/cancers18203351 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 16;18(20):3351. doi: 10.3390/cancers18203351. PMID- 42938882 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 16 TI - The Evolution of Neoadjuvant Treatment for Resectable Esophageal Squamous Cell Carcinoma: Past Achievements, Current Standards, and Future Perspectives. LID - 10.3390/cancers18203350 [doi] LID - 3350 AB - Neoadjuvant treatment has become an integral component of multidisciplinary management for resectable esophageal squamous cell carcinoma (ESCC). Over recent decades, treatment has evolved from surgery alone to postoperative chemotherapy, neoadjuvant chemotherapy, intensified systemic therapy, neoadjuvant chemoradiotherapy, and, more recently, the incorporation of immune checkpoint inhibitors into curative-intent treatment. This evolution has been driven by persistent challenges including systemic recurrence, locoregional failure, treatment-related toxicity, and heterogeneous treatment response. This review traces the evolution of neoadjuvant treatment for resectable ESCC through landmark clinical trials that have shaped current practice. We discuss the transition from postoperative to neoadjuvant chemotherapy, the development of intensified chemotherapy and neoadjuvant chemoradiotherapy, and the emergence of immunotherapy, distinguishing the established role of adjuvant nivolumab from the emerging role of neoadjuvant immunochemotherapy. We then examine the unresolved relative roles of these strategies and the challenges in treatment selection. Finally, we discuss future directions centered on accurate response assessment, circulating tumor DNA-based molecular residual disease assessment, and response-adapted treatment, with particular emphasis on organ-preserving strategies after a clinical complete response. Although active surveillance has emerged as a potential alternative to immediate surgery in carefully selected patients, accurate identification of residual disease and appropriate surveillance remain essential. Future advances may therefore shift the focus from treatment intensification alone toward response-adapted strategies that can maintain oncological safety while potentially enabling organ preservation. FAU - Ujiie, Naoto AU - Ujiie N AUID- ORCID: 0000-0001-9431-0912 AD - Department of Surgery, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Miyagi, Japan. FAU - Taniyama, Yusuke AU - Taniyama Y AUID- ORCID: 0000-0003-2563-247X AD - Department of Surgery, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Miyagi, Japan. FAU - Sato, Chiaki AU - Sato C AUID- ORCID: 0000-0003-1449-2053 AD - Department of Surgery, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Miyagi, Japan. FAU - Okamoto, Hiroshi AU - Okamoto H AUID- ORCID: 0000-0002-9042-7096 AD - Department of Surgery, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Miyagi, Japan. FAU - Ozawa, Yohei AU - Ozawa Y AD - Department of Surgery, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Miyagi, Japan. FAU - Ishida, Hirotaka AU - Ishida H AD - Department of Surgery, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Miyagi, Japan. FAU - Unno, Michiaki AU - Unno M AD - Department of Surgery, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Miyagi, Japan. FAU - Kamei, Takashi AU - Kamei T AD - Department of Surgery, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Miyagi, Japan. LA - eng PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702641 OTO - NOTNLM OT - esophageal squamous cell carcinoma OT - immunotherapy OT - neoadjuvant chemoradiotherapy OT - neoadjuvant chemotherapy OT - response-adapted treatment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/16 CRDT- 2026/10/27 10:31 PHST- 2026/09/28 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/15 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - cancers18203350 [pii] AID - cancers-18-03350 [pii] AID - 10.3390/cancers18203350 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 16;18(20):3350. doi: 10.3390/cancers18203350. PMID- 42938881 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 16 TI - Liquid Biopsy in Hepatobiliary and Pancreatic Malignancies: Current Evidence and Implications for Surgical Practice. LID - 10.3390/cancers18203349 [doi] LID - 3349 AB - Liquid biopsy is an emerging approach for the molecular characterization of hepatopancreatobiliary (HPB) malignancies through the analysis of circulating tumor DNA (ctDNA), circulating tumor cells (CTCs), and extracellular vesicles (EVs). These biomarkers may complement conventional imaging and serum markers by providing additional information on tumor biology and disease evolution. This narrative review examines the available evidence concerning the potential applications of liquid biopsy in HPB surgical practice, with particular emphasis on preoperative prognostic assessment, postoperative molecular residual disease detection, recurrence monitoring, and treatment adaptation. Among the different circulating biomarkers, ctDNA currently has the most developed perioperative evidence base. Studies involving pancreatic ductal adenocarcinoma, hepatocellular carcinoma, and biliary tract cancers have demonstrated associations between ctDNA detection and unfavorable oncological outcomes, particularly postoperative recurrence. Nevertheless, the available evidence remains predominantly observational, with considerable heterogeneity in patient populations, sampling protocols, analytical platforms, and outcome definitions. Although the integration of circulating biomarkers with conventional anatomical criteria has generated interest in the concept of "biological resectability", this framework remains hypothetical and has not been prospectively validated for surgical treatment selection. Similarly, ctDNA-guided modifications of neoadjuvant therapy, adjuvant treatment, or postoperative surveillance have not yet demonstrated clinical benefit in HPB malignancies. Liquid biopsy should therefore currently be regarded as a promising investigational prognostic tool rather than an established determinant of surgical or oncological management. Standardized methodologies and prospective interventional trials are required before its routine clinical implementation. FAU - Caringi, Silvio AU - Caringi S AUID- ORCID: 0009-0008-6007-8834 AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva Delle Fonti, Italy. FAU - Delvecchio, Antonella AU - Delvecchio A AUID- ORCID: 0000-0002-7759-4340 AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva Delle Fonti, Italy. AD - Department of Medicine and Surgery, LUM University, 70010 Casamassima, Italy. FAU - Casella, Annachiara AU - Casella A AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva Delle Fonti, Italy. FAU - Ferraro, Valentina AU - Ferraro V AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva Delle Fonti, Italy. FAU - Stasi, Matteo AU - Stasi M AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva Delle Fonti, Italy. FAU - Tralli, Nunzio AU - Tralli N AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva Delle Fonti, Italy. FAU - Manzia, Tommaso Maria AU - Manzia TM AUID- ORCID: 0000-0002-4636-3478 AD - Transplant and HPB Unit, Department of Surgery Sciences, University of Rome Tor Vergata, 00133 Rome, Italy. FAU - Inchingolo, Riccardo AU - Inchingolo R AUID- ORCID: 0000-0002-0253-5936 AD - Department of Medicine and Surgery, LUM University, 70010 Casamassima, Italy. AD - Unit of Interventional Radiology, F. Miulli Hospital, 70021 Acquaviva Delle Fonti, Italy. FAU - Tedeschi, Michele AU - Tedeschi M AUID- ORCID: 0000-0002-2101-5418 AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva Delle Fonti, Italy. AD - Department of Medicine and Surgery, LUM University, 70010 Casamassima, Italy. FAU - Memeo, Riccardo AU - Memeo R AUID- ORCID: 0000-0002-1668-932X AD - Unit of Hepato-Biliary and Pancreatic Surgery, "F. Miulli" General Hospital, 70021 Acquaviva Delle Fonti, Italy. AD - Department of Medicine and Surgery, LUM University, 70010 Casamassima, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702558 OTO - NOTNLM OT - circulating tumor DNA OT - hepatobiliary and pancreatic malignancies OT - liquid biopsy OT - molecular residual disease OT - surgical decision-making COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/16 CRDT- 2026/10/27 10:31 PHST- 2026/09/01 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/16 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - cancers18203349 [pii] AID - cancers-18-03349 [pii] AID - 10.3390/cancers18203349 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 16;18(20):3349. doi: 10.3390/cancers18203349. PMID- 42938880 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 16 TI - Feasibility Is Not Benefit: Resectable, Irradiable, or Biologically Selected? A Critical Review of the Unresolved Local-Platform Question in Stage III/N2 NSCLC. LID - 10.3390/cancers18203348 [doi] LID - 3348 AB - Stage III/N2 non-small-cell lung cancer (NSCLC) can often be treated with either surgery-based multimodality therapy or definitive chemoradiotherapy. Historical randomized trials showed that surgery improved local control without demonstrating an overall-survival benefit; neither platform demonstrated superiority, although none was designed to establish equivalence. In the immunotherapy era, both pathways have improved through separate evidence streams: perioperative trials compared immunotherapy with chemotherapy-based surgical controls, and PACIFIC compared durvalumab with placebo after chemoradiotherapy. Neither stream determines which complete modern pathway is better for a patient eligible for both, and cross-trial comparisons are confounded by differences in populations, randomization points, and endpoint definitions. This review distinguishes three questions that are often conflated: whether direct comparative evidence exists, how large any relative treatment effect may be, and whether sufficient clinical equipoise exists to justify randomization. Technical resectability, physiological operability, and irradiability define eligibility for a platform, not comparative benefit, and no current biomarker has been shown to modify the relative effect of the two platforms. Comparative uncertainty is most visible in multistation, bulky, or anatomically complex N2 disease, the most practicable starting point for randomization; superiority of either platform, however, has not been demonstrated at either end of the N2 spectrum. A staged randomized comparison of the complete modern pathways, with prespecified analyses of candidate treatment-effect modifiers, is proposed as a reasoned research strategy. FAU - Skarlos, Panteleimonas AU - Skarlos P AUID- ORCID: 0009-0008-4913-3097 AD - Department of Radiation Oncology, University Hospital of Larissa, Medical School, University of Thessaly, 41110 Larissa, Greece. FAU - Georgakopoulos, Ioannis AU - Georgakopoulos I AUID- ORCID: 0000-0002-6354-9805 AD - Radiation Oncology Department, ARETAIEION University Hospital, Medical School, Kapodistrian University of Athens, 11528 Athens, Greece. FAU - Kougioumtzopoulou, Andromachi AU - Kougioumtzopoulou A AUID- ORCID: 0000-0003-3576-5495 AD - Department of Clinical Radiation Oncology, University Hospital ATTIKON, Medical School, Kapodistrian University of Athens, 12462 Athens, Greece. FAU - Liakouli, Zoi AU - Liakouli Z AD - Department of Radiation Oncology, University Hospital of Larissa, Medical School, University of Thessaly, 41110 Larissa, Greece. FAU - Theodorou, Kiki AU - Theodorou K AUID- ORCID: 0000-0002-6571-9433 AD - Department of Medical Physics, University Hospital of Larissa, Medical School, University of Thessaly, 41110 Larissa, Greece. FAU - Zygogianni, Anna AU - Zygogianni A AD - Department of Clinical Radiation Oncology, University Hospital ATTIKON, Medical School, Kapodistrian University of Athens, 12462 Athens, Greece. FAU - Georgiannakis, Emmanouil AU - Georgiannakis E AD - Department of Minimally Invasive Thoracic Surgery, Metropolitan Hospital, 18547 Piraeus, Greece. AD - Department of Thoracic Surgery, Metropolitan General, 15562 Athens, Greece. AD - Department of Basic and Clinical Sciences, Medical School, University of Nicosia (UNIC), Athens Campus, 16777 Athens, Greece. FAU - Kouloulias, Vasileios AU - Kouloulias V AUID- ORCID: 0000-0003-2082-7323 AD - Department of Clinical Radiation Oncology, University Hospital ATTIKON, Medical School, Kapodistrian University of Athens, 12462 Athens, Greece. FAU - Maravelis, George AU - Maravelis G AD - 2nd Department of Radiation Oncology, Metropolitan Hospital, Neo Faliro, 18547 Piraeus, Greece. LA - eng PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702213 OTO - NOTNLM OT - N2 NSCLC OT - TNM ninth edition OT - chemoradiotherapy OT - clinical equipoise OT - durvalumab OT - perioperative immunotherapy OT - randomized trial OT - resectability OT - stage III NSCLC OT - treatment selection COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/16 CRDT- 2026/10/27 10:31 PHST- 2026/09/13 00:00 [received] PHST- 2026/10/13 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - cancers18203348 [pii] AID - cancers-18-03348 [pii] AID - 10.3390/cancers18203348 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 16;18(20):3348. doi: 10.3390/cancers18203348. PMID- 42938879 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 16 TI - Peritumoral Radiomic Deviation on DCE-MRI Exceeds the Bilateral Normal-Appearing Parenchymal Distance in Breast Cancer: A Within-Patient Multi-Metric Study. LID - 10.3390/cancers18203347 [doi] LID - 3347 AB - BACKGROUND: The imaging-defined peritumoral region may contain quantitative imaging information beyond that captured by the tumor core, but its radiomic deviation from normal-appearing breast parenchyma remains insufficiently characterized. This retrospective single-center study investigated whether peritumoral-to-normal radiomic distances exceed the within-patient bilateral normal-appearing parenchymal reference distance. Fifty-six patients with histopathologically confirmed breast cancer and preoperative bilateral dynamic contrast-enhanced MRI were included. METHODS: Single-slice radiomic analysis was performed for four predefined regions: tumor core (T), peritumoral microenvironment (M), remote ipsilateral normal-appearing parenchyma (I), and contralateral normal-appearing parenchyma (C). After quality control, 101 radiomic features were standardized and analyzed using standardized Euclidean, Manhattan, cosine, and Ledoit-Wolf Mahalanobis distances. Peritumoral-to-ipsilateral (dMI) and peritumoral-to-contralateral (dMC) distances were compared with the within-patient bilateral normal-appearing parenchymal distance (dIC). RESULTS: Across all four metrics, both dMI and dMC exceeded dIC, and all eight primary paired Wilcoxon comparisons remained significant after Benjamini-Hochberg correction (q/=3 chemotherapy-related adverse events occurred in 15 patients (50.0%), immune-related adverse events occurred in 11 patients (36.6%), and Grade 3 or higher immune-related adverse events occurred in four patients (13.3%). Pembrolizumab was suspended in six patients (20.0%), while all 30 patients proceeded to definitive surgery. In the dose-dense chemotherapy cohort, the pCR rate was 31.0%, with a grade >/=3 chemotherapy-related adverse event rate of 20.7%, and all patients proceeded to definitive surgery. CONCLUSIONS: In this descriptive real-world single-centre Hong Kong study, the neoadjuvant KN522 regimen was feasible in Chinese patients with early TNBC, although treatment-related toxicity and treatment modification were common. The observed pCR rate was lower than that reported in the original KN522 trial, possibly reflecting differences in patient selection and real-world treatment delivery patterns. Larger multi-centre studies are needed to evaluate outcomes in Chinese and other Asian populations. FAU - Lo, Yuk-Fong Grace AU - Lo YG AD - Department of Clinical Oncology, Pamela Youde Nethersole Eastern Hospital, Hong Kong SAR, China. FAU - Chan, Kuen AU - Chan K AD - Department of Clinical Oncology, Pamela Youde Nethersole Eastern Hospital, Hong Kong SAR, China. FAU - Soong, Sung Inda AU - Soong SI AUID- ORCID: 0000-0002-8911-8798 AD - Department of Clinical Oncology, Pamela Youde Nethersole Eastern Hospital, Hong Kong SAR, China. LA - eng PT - Journal Article DEP - 20261016 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702728 OTO - NOTNLM OT - Chinese OT - Hong Kong OT - KEYNOTE522 OT - neoadjuvant chemotherapy OT - pathological complete response OT - pembrolizumab OT - real-world OT - triple-negative breast cancer COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/16 CRDT- 2026/10/27 10:31 PHST- 2026/09/20 00:00 [received] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - cancers18203339 [pii] AID - cancers-18-03339 [pii] AID - 10.3390/cancers18203339 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 16;18(20):3339. doi: 10.3390/cancers18203339. PMID- 42938871 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 16 TI - Liquid Biopsy-Guided Surgical Decision-Making in Oligometastatic Non-Small Cell Lung Cancer: Toward Dynamic Risk Stratification in the Multidisciplinary Era. LID - 10.3390/cancers18203338 [doi] LID - 3338 AB - Oligometastatic non-small cell lung cancer (NSCLC) is defined anatomically by a threshold of five or fewer metastatic lesions across three or fewer organs. That definition is a proxy for a biological question it cannot answer: whether the metastatic process has been arrested at a stage where eradication of all detectable disease is curative. Randomized phase II trials of local consolidative therapy have shown median progression-free survival gains of 6.2 and 8.0 months and, in one trial, a median overall survival of 41.2 months vs. 17.0 months, yet median progression-free survival with local therapy did not exceed 14.2 months, and the phase II/III NRG-LU002 trial found no significant survival benefit from adding local therapy to maintenance therapy. Circulating tumor DNA (ctDNA) measures molecular disease burden. In resectable NSCLC, preoperative ctDNA detection is associated with a higher risk of relapse; in oligometastatic disease, the baseline sample is instead the quantitative reference for response. ctDNA kinetics during induction therapy track systemic response before any operation, although no response threshold has been validated, and postoperative molecular residual disease anticipates radiological relapse by a median of approximately 5 months. This review synthesizes the evidence linking liquid biopsy to surgical decision-making and proposes, as a hypothesis for prospective testing, a dynamic risk stratification framework anchored on five plasma sampling points. We separate established prognostic evidence from untested hypotheses, address oncogene-driven and other oligometastatic states, and define the assay requirements, confounders and trial designs needed before ctDNA can select patients for or against thoracic resection. FAU - Magouliotis, Dimitrios E AU - Magouliotis DE AUID- ORCID: 0000-0001-5417-6392 AD - Department of Cardiac Surgery Research, Lankenau Institute for Medical Research, Main Line Health, Wynnewood, PA 19096, USA. FAU - Androutsopoulou, Vasiliki AU - Androutsopoulou V AUID- ORCID: 0009-0005-2445-0879 AD - Department of Cardiothoracic Surgery, University of Thessaly, Biopolis, 41110 Larissa, Greece. FAU - Zotos, Prokopis-Andreas AU - Zotos PA AD - Department of Cardiothoracic Surgery, University of Thessaly, Biopolis, 41110 Larissa, Greece. FAU - Abbaker, Namariq AU - Abbaker N AUID- ORCID: 0000-0002-7317-3215 AD - Department of Surgery and Cancer, Hammersmith Hospital, Imperial College Healthcare NHS Trust, London W12 0HS, UK. FAU - Cioffi, Ugo AU - Cioffi U AUID- ORCID: 0000-0002-5321-5828 AD - Department of Surgery, University of Milan, 20157 Milan, Italy. FAU - Minervini, Fabrizio AU - Minervini F AUID- ORCID: 0000-0003-3595-7307 AD - Department of Thoracic Surgery, Cantonal Hospital Lucerne, 6000 Lucerne, Switzerland. FAU - Scarci, Marco AU - Scarci M AUID- ORCID: 0000-0002-4459-7721 AD - Department of Surgery and Cancer, Hammersmith Hospital, Imperial College Healthcare NHS Trust, London W12 0HS, UK. LA - eng PT - Journal Article PT - Review DEP - 20261016 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702110 OTO - NOTNLM OT - circulating tumor DNA OT - dynamic risk stratification OT - liquid biopsy OT - minimal residual disease OT - multidisciplinary team OT - non-small cell lung cancer OT - oligometastatic disease OT - thoracic surgery COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/16 CRDT- 2026/10/27 10:31 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/16 00:00 [pmc-release] AID - cancers18203338 [pii] AID - cancers-18-03338 [pii] AID - 10.3390/cancers18203338 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 16;18(20):3338. doi: 10.3390/cancers18203338. PMID- 42938870 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 15 TI - Machine Learning-Guided Virtual Screening Identifies a Novel MELK-Targeted Antiproliferative Lead Compound with G2/M Arrest Activity in Triple-Negative Breast Cancer and Lung Adenocarcinoma Cells. LID - 10.3390/cancers18203336 [doi] LID - 3336 AB - Background: Maternal embryonic leucine zipper kinase (MELK) is a cell cycle-regulated serine/threonine kinase frequently overexpressed in human malignancies, and represents a promising anticancer drug target. Discovery of structurally novel, highly selective MELK inhibitors remains an unmet need. Methods: In the study, we implemented an integrated computational pipeline combining four machine learning regressors (Random Forest, Gradient Boosting, XGBoost, LightGBM), hierarchical Glide docking (HTVS to SP to XP), MM/GBSA rescoring, ADMET prediction, and 100 ns molecular dynamics simulations to screen a ~15.58 million-compound commercial library, yielding 12 prioritized candidates. Three commercially available compounds (CPD4, CPD6, CPD10) were evaluated in an antiproliferative panel. Results: CPD6 was identified as the most active hit, with IC(50) values of 1.44 muM in MDA-MB-468 and 1.66 muM in H1299, modestly exceeding the reference compound MELK-8a under the same assay conditions (2.48 and 2.51 muM, respectively). Cellular thermal shift assay in cell lysates provided biochemical evidence of MELK engagement, and 100 ns molecular dynamics simulations supported a computationally predicted binding mode in the MELK kinase domain. Additional substrate phosphorylation and MELK overexpression rescue assays further supported MELK-dependent inhibitory activity. Colony formation, EdU incorporation, and cell cycle analyses demonstrated dose-dependent antiproliferative effects and G2/M arrest, with concomitant downregulation of CDC25C, CDK1, and Cyclin B1. Discussion: CPD6 is a structurally novel MELK lead distinct from reported inhibitors. While our data support MELK target engagement, kinome selectivity profiling remains required, and this ML pipeline demonstrates the utility of AI-driven large-scale anticancer lead screening. Conclusions: Using the integrated machine-learning virtual screening pipeline established in this work, we identified CPD6 as a structurally novel MELK-targeted lead that engages MELK, induces G2/M arrest, and exerts low-micromolar antiproliferative activity in triple-negative breast cancer and lung adenocarcinoma models. FAU - Cao, Wanxin AU - Cao W AD - Beijing Key Laboratory of Innovative Drug Discovery and Polymorphic Druggability Research for Cerebrovascular Diseases, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. FAU - Jiang, Bin AU - Jiang B AD - Beijing Key Laboratory of Innovative Drug Discovery and Polymorphic Druggability Research for Cerebrovascular Diseases, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. FAU - Chi, Xiangyin AU - Chi X AD - Beijing Key Laboratory of Innovative Drug Discovery and Polymorphic Druggability Research for Cerebrovascular Diseases, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. FAU - Li, Wan AU - Li W AD - Beijing Key Laboratory of Innovative Drug Discovery and Polymorphic Druggability Research for Cerebrovascular Diseases, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. FAU - Yang, Hong AU - Yang H AD - Beijing Key Laboratory of Innovative Drug Discovery and Polymorphic Druggability Research for Cerebrovascular Diseases, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. FAU - Li, Xiaoxue AU - Li X AD - School of Clinical Pharmacy, Shenyang Pharmaceutical University, Shenyang 117004, China. FAU - Zhao, Yuxuan AU - Zhao Y AD - School of Pharmacy, Henan University, Kaifeng 475004, China. FAU - Zhang, Xu AU - Zhang X AD - Beijing Key Laboratory of Innovative Drug Discovery and Polymorphic Druggability Research for Cerebrovascular Diseases, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. FAU - Meng, Rui AU - Meng R AD - Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan 030006, China. FAU - Cang, Yanyu AU - Cang Y AD - Beijing Key Laboratory of Innovative Drug Discovery and Polymorphic Druggability Research for Cerebrovascular Diseases, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. FAU - Du, Guanhua AU - Du G AD - Beijing Key Laboratory of Innovative Drug Discovery and Polymorphic Druggability Research for Cerebrovascular Diseases, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. FAU - Wang, Jinhua AU - Wang J AUID- ORCID: 0000-0001-8367-0787 AD - Beijing Key Laboratory of Innovative Drug Discovery and Polymorphic Druggability Research for Cerebrovascular Diseases, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China. LA - eng GR - GN2024-31-4700/2024 China Industrial Technology Infrastructure Public Service Platform Project/ GR - 7252202, 25JL008/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702308 OTO - NOTNLM OT - CETSA OT - MELK OT - lung adenocarcinoma OT - machine learning OT - triple-negative breast cancer OT - virtual screening COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/15 CRDT- 2026/10/27 10:31 PHST- 2026/09/02 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/02 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - cancers18203336 [pii] AID - cancers-18-03336 [pii] AID - 10.3390/cancers18203336 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 15;18(20):3336. doi: 10.3390/cancers18203336. PMID- 42938869 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 15 TI - Liver Transplantation for Fibrolamellar Carcinoma: National Registry Analysis and Reported Outcomes. LID - 10.3390/cancers18203335 [doi] LID - 3335 AB - Background/Objectives: Liver transplantation may provide prolonged survival in fibrolamellar carcinoma (FLC), but the frequency and timing of post-transplant recurrence, and the basis for patient selection, remain poorly defined. We described the course from listing through long-term follow-up and tested whether routine pretransplant measurements distinguished the recurrence risk. Methods: Scientific Registry of Transplant Recipients data identified separate listing and first-transplant cohorts during 2002-2025. Competing-risk analyses assessed waiting-list outcomes and reported recurrence of the pretransplant tumour. Exploratory recurrence models were internally validated with 500 patient bootstrap samples, with performance assessed only in omitted recipients. Recorded explant tumour extent was examined in the registry's liver explant file. Results: This study included 61 listed candidates and 51 first-transplant recipients; 42 recipients had a pathology-supported discharge diagnosis of FLC. One-year incidence of transplantation was 76.5% (95% CI 65.9-86.6). After a median potential follow-up of 8.1 years, five-year post-transplant survival was 63.3% (95% CI 46.4-76.2). Seventeen recurrences were reported and the three-year cumulative incidence was 26.1% (95% CI 13.9-39.1). Malignancy was the recorded primary cause in 13 of 23 deaths. Laboratory-component and compact clinical models had mean three-year validation areas under the curve of 0.571 and 0.507, and neither reduced prediction error relative to its matched cohort-average prediction. Conclusions: Transplantation was followed by prolonged survival in selected patients with FLC, although reported recurrence was common and sometimes late. Routine pretransplant variables did not identify reproducible recurrence-risk groups. With 51 recipients and 17 recurrences, these estimates are imprecise and the prediction analyses are exploratory; selection should remain multidisciplinary while tumour extent, treatment response and biological markers are evaluated prospectively. FAU - Gupta, Piyush AU - Gupta P AUID- ORCID: 0000-0002-3860-2512 AD - MedStar Georgetown Transplant Institute, MedStar Georgetown University Hospital, 3800 Reservoir Road NW, Washington, DC 20007, USA. FAU - Moris, Dimitrios AU - Moris D AUID- ORCID: 0000-0002-5276-0699 AD - MedStar Georgetown Transplant Institute, MedStar Georgetown University Hospital, 3800 Reservoir Road NW, Washington, DC 20007, USA. FAU - Mea, Caitlin AU - Mea C AD - Department of Surgery, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA. FAU - Walls, David AU - Walls D AUID- ORCID: 0000-0003-2508-571X AD - MedStar Georgetown Transplant Institute, MedStar Georgetown University Hospital, 3800 Reservoir Road NW, Washington, DC 20007, USA. FAU - Gogna, Shekhar AU - Gogna S AUID- ORCID: 0000-0002-4075-9674 AD - MedStar Georgetown Transplant Institute, MedStar Georgetown University Hospital, 3800 Reservoir Road NW, Washington, DC 20007, USA. FAU - Aguirre, Oswaldo AU - Aguirre O AD - MedStar Georgetown Transplant Institute, MedStar Georgetown University Hospital, 3800 Reservoir Road NW, Washington, DC 20007, USA. FAU - Ziogas, Ioannis A AU - Ziogas IA AD - Department of Surgery, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA. FAU - Nguyen, Brian M AU - Nguyen BM AD - MedStar Georgetown Transplant Institute, MedStar Georgetown University Hospital, 3800 Reservoir Road NW, Washington, DC 20007, USA. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702327 OTO - NOTNLM OT - competing risks OT - fibrolamellar carcinoma OT - liver transplantation OT - prediction model OT - recurrence OT - scientific registry of transplant recipients OT - transplant oncology COIS- D.M. is a Guest Editor of the Special Issue to which this manuscript is submitted. In accordance with the journal's Special Issue guidelines, he was recused from handling this manuscript, an independent Editorial Board Member acted as Academic Editor, and he had no access to the peer review other than in his capacity as an author. The authors declare no other conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/15 CRDT- 2026/10/27 10:31 PHST- 2026/09/21 00:00 [received] PHST- 2026/10/14 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - cancers18203335 [pii] AID - cancers-18-03335 [pii] AID - 10.3390/cancers18203335 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 15;18(20):3335. doi: 10.3390/cancers18203335. PMID- 42938868 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 15 TI - Real-World Management and Survival Outcomes in Patients with Primary or Recurrent Retroperitoneal Liposarcoma: A Single-Center Retrospective Cohort Study. LID - 10.3390/cancers18203334 [doi] LID - 3334 AB - Background/Objectives: Histological subtype and resection completeness are established prognostic factors in retroperitoneal liposarcoma, but evidence often derives from resected primary disease. We characterized management and post-referral survival in a referral cohort. Methods: We retrospectively analyzed 61 patients managed from May 2015 to September 2025. Overall survival (OS) was measured from first presentation at our center. Kaplan-Meier and exploratory Cox analyses were performed, allowing the association between tumor diameter and mortality to vary over time. Results: Forty-two patients had primary disease, and 19 had recurrent disease; 12 had well-differentiated liposarcoma (WDLPS), and 49 had dedifferentiated liposarcoma (DDLPS). Among 24 patients who underwent surgery, 21 had macroscopically complete (R0/R1) resection. Twenty-five patients died. No deaths occurred in WDLPS; median OS was 29.8 months in DDLPS (p = 0.008). In DDLPS, recurrent versus primary disease was not associated with OS (hazard ratio [HR], 0.99; 95% confidence interval [CI], 0.41-2.38). Eastern Cooperative Oncology Group (ECOG) performance status >/= 2 (n = 6 in the entire cohort) was associated with poorer OS (adjusted HR, 8.03; 95% CI, 2.20-29.36), and the association between tumor diameter and mortality varied over time (adjusted interaction HR, 1.44; 95% CI, 1.07-1.95). Conclusions: Observed post-referral survival differed by histological subtype. Among patients with DDLPS, no survival difference between primary and recurrent disease was demonstrated, but the wide CI and referral-related survivor selection preclude an inference of equivalence. Associations involving performance status and time-varying tumor diameter were exploratory and require external validation. FAU - Kijima, Atsuhiro AU - Kijima A AD - Department of Urology, National Hospital Organization Kyushu Cancer Center, Notame 3-1-1, Minami-ku, Fukuoka 811-1395, Japan. FAU - Furubayashi, Nobuki AU - Furubayashi N AUID- ORCID: 0000-0001-9159-493X AD - Department of Urology, National Hospital Organization Kyushu Cancer Center, Notame 3-1-1, Minami-ku, Fukuoka 811-1395, Japan. FAU - Negishi, Takahito AU - Negishi T AD - Department of Urology, National Hospital Organization Kyushu Cancer Center, Notame 3-1-1, Minami-ku, Fukuoka 811-1395, Japan. FAU - Nakamura, Motonobu AU - Nakamura M AD - Department of Urology, National Hospital Organization Kyushu Cancer Center, Notame 3-1-1, Minami-ku, Fukuoka 811-1395, Japan. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702551 OTO - NOTNLM OT - dedifferentiated liposarcoma OT - overall survival OT - performance status OT - prognostic factors OT - real-world evidence OT - retroperitoneal liposarcoma OT - tumor size OT - well-differentiated liposarcoma COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/15 CRDT- 2026/10/27 10:31 PHST- 2026/09/26 00:00 [received] PHST- 2026/10/12 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - cancers18203334 [pii] AID - cancers-18-03334 [pii] AID - 10.3390/cancers18203334 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 15;18(20):3334. doi: 10.3390/cancers18203334. PMID- 42938864 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 15 TI - Early Local Tumour Control and Feasibility of Intratumoral P-32 Microparticle Implantation in Patients with Locally Advanced Pancreatic Cancer: A Prospective Single-Center Observational Cohort Study. LID - 10.3390/cancers18203331 [doi] LID - 3331 AB - Background: Evidence regarding EUS-guided intratumoral phosphorus-32 (P-32) microparticle implantation for locally advanced pancreatic cancer (LAPC) remains limited. This prospective pilot study evaluated its technical feasibility, preliminary safety, and early disease-control outcomes when administered in combination with gemcitabine plus nab-paclitaxel. Methods: Seventeen patients with unresectable LAPC underwent EUS-guided intratumoral P-32 microparticle implantation through a 22-gauge fine-needle aspiration needle during the fourth week of systemic chemotherapy. Intratumoral distribution was assessed using Bremsstrahlung SPECT/CT. Tumour response was evaluated according to RECIST version 1.1, and treatment-emergent adverse events were graded according to CTCAE version 4.0. The main secondary endpoint was local disease control rate at approximately 16 weeks (LDCR16w). All analyses were descriptive. Results: Implantation was technically successful in all 17 patients (100.0%; exact 95% CI, 80.5-100.0%), and SPECT/CT confirmed intratumoral distribution in every case. No implantation-related procedural complications were recorded (0/17; exact 95% CI, 0.0-19.5%). At approximately 16 weeks, CT assessment was available for 16 patients: seven had a partial response and nine had stable disease. The LDCR16w was 16/16 among evaluable patients (100.0%; exact 95% CI, 79.4-100.0%) and 16/17 in the all-treated analysis (94.1%; exact 95% CI, 71.3-99.9%). A reduction in CA19-9 of at least 50% was observed in 13/16 evaluable patients. Four patients underwent subsequent R0 resection (4/17, 23.5%; exact 95% CI, 6.8-49.9%). Adverse events were predominantly grade 1-2; one grade 3 abdominal pain event and no grade 4-5 events were recorded. Only three deaths occurred during a median OS follow-up of 15.6 months, making survival estimates exploratory and statistically immature. Conclusions: In this small, single-center pilot cohort of highly selected patients, EUS-guided intratumoral P-32 microparticle implantation combined with gemcitabine plus nab-paclitaxel was technically feasible and provided preliminary safety information. Because of the absence of a chemotherapy-only comparator, the small sample size, and the limited follow-up, the radiographic, biomarker, resection, and survival outcomes cannot be attributed specifically to P-32 implantation and should be considered descriptive and hypothesis-generating. Larger controlled studies with longer follow-up are required to determine clinical efficacy. FAU - Kontos, Athanasios AU - Kontos A AUID- ORCID: 0009-0008-7514-0434 AD - Endoscopy Unit, Gastroenterology Department, Hospital Universitario Rio Hortega, 47012 Valladolid, Spain. FAU - Riris, Ioannis AU - Riris I AUID- ORCID: 0009-0009-0725-3696 AD - Medical School of Athens, National and Kapodistrian University of Athens, 11527 Athens, Greece. FAU - Kypraios, Dimitrios AU - Kypraios D AUID- ORCID: 0009-0009-0515-1215 AD - Gastroenterology Department, Agios Savvas Hospital, 11522 Athens, Greece. FAU - Alexakis, Nikolaos AU - Alexakis N AUID- ORCID: 0000-0001-7774-054X AD - First Propaedeutic Department of Surgery, School of Medicine, National and Kapodistrian University of Athens, Hippocration General Hospital of Athens, 11527 Athens, Greece. FAU - Zografos, Georgios AU - Zografos G AUID- ORCID: 0000-0002-5429-8776 AD - First Propaedeutic Department of Surgery, School of Medicine, National and Kapodistrian University of Athens, Hippocration General Hospital of Athens, 11527 Athens, Greece. FAU - Toutouzas, Konstantinos AU - Toutouzas K AUID- ORCID: 0000-0002-7830-6945 AD - First Propaedeutic Department of Surgery, School of Medicine, National and Kapodistrian University of Athens, Hippocration General Hospital of Athens, 11527 Athens, Greece. LA - eng GR - Oncosil Medical Europe/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702592 OTO - NOTNLM OT - brachytherapy OT - intratumoral therapy OT - local tumour control OT - pancreatic cancer OT - phosphorus-32 OT - radioactive microparticles OT - surgical resection COIS- The study received funding from OncoSil Medical Ltd. The sponsor had no involvement in the study design, data collection, data analysis, interpretation of the results, manuscript preparation, or the decision to publish the results. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/15 CRDT- 2026/10/27 10:31 PHST- 2026/08/19 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - cancers18203331 [pii] AID - cancers-18-03331 [pii] AID - 10.3390/cancers18203331 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 15;18(20):3331. doi: 10.3390/cancers18203331. PMID- 42938863 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 15 TI - Immunoscore and Post-Neoadjuvant Pathological Features in Microsatellite-Stable Locally Advanced Gastric Cancer: A Retrospective Cohort Study. LID - 10.3390/cancers18203329 [doi] LID - 3329 AB - Background/Objectives: Pathological response after neoadjuvant chemotherapy is an established prognostic factor in locally advanced gastric cancer, but it does not fully reflect the biological heterogeneity of residual disease. The immune microenvironment may provide additional prognostic information. We therefore investigated the relationship between post-neoadjuvant Immunoscore, pathological findings, and disease-free survival in patients with microsatellite-stable locally advanced gastric cancer. Methods: This retrospective, single-center study included patients with locally advanced gastric adenocarcinoma who received four cycles of neoadjuvant FLOT followed by curative-intent gastrectomy between November 2023 and May 2025. Immunoscore was determined in post-neoadjuvant resection specimens based on CD3(+) and CD8(+) T-cell infiltration in the tumor center and invasive margin. Immunoscore assessments were performed by two pathologists blinded to clinical and outcome data, with final evaluations established through consensus review. Associations between Immunoscore and clinicopathological features were examined, including exploratory multivariable analyses adjusting for baseline clinical T stage and histological subtype. Disease-free survival was analyzed using the Kaplan-Meier method and Cox proportional hazards regression. Results: The final analysis included 48 patients. Compared with the low-Immunoscore group, the high-Immunoscore group had lower rates of ypT4 disease (37.5% vs. 75.0%; p = 0.014), ypN3 disease (21.9% vs. 68.8%; p = 0.002), and perineural invasion (43.8% vs. 75.0%; p = 0.041). The association between low Immunoscore and ypN3 disease persisted after adjustment for baseline clinical T stage and histological subtype (adjusted OR 5.89, 95% CI 1.32-26.23; p = 0.020). Tumor regression grade did not differ significantly between the groups (p = 0.211). During a median follow-up of 18.0 months, 17 patients developed recurrence. Disease-free survival was numerically longer in the high-Immunoscore group. The difference was not significant by the log-rank test (p = 0.100), whereas an exploratory Breslow analysis showed a difference between the survival distributions (p = 0.025). However, Cox regression did not demonstrate a statistically significant association with disease-free survival (HR 0.47, 95% CI 0.18-1.21; p = 0.117). Conclusions: A higher post-neoadjuvant Immunoscore was associated with less advanced residual tumor and nodal disease and less frequent perineural invasion, without a statistically significant association with tumor regression grade or disease-free survival in the primary analyses. Immunoscore may therefore complement conventional pathological assessment after neoadjuvant FLOT. These findings require validation in larger prospective cohorts. FAU - Kutlu, Ozlem AU - Kutlu O AUID- ORCID: 0000-0001-5050-3333 AD - Department of Medical Oncology, Izmir City Hospital, Izmir 35540, Turkey. FAU - Gul, Gurkan AU - Gul G AUID- ORCID: 0000-0001-8474-6763 AD - Department of Medical Oncology, Izmir City Hospital, Izmir 35540, Turkey. FAU - Baran, Simge AU - Baran S AD - Department of Pathology, Izmir City Hospital, Izmir 35540, Turkey. FAU - Argon, Asuman AU - Argon A AUID- ORCID: 0000-0001-7406-0610 AD - Department of Pathology, Izmir City Hospital, Izmir 35540, Turkey. FAU - Esim, Damla Gunenc AU - Esim DG AUID- ORCID: 0000-0002-7592-2021 AD - Department of Medical Oncology, Izmir City Hospital, Izmir 35540, Turkey. FAU - Taskaynatan, Halil AU - Taskaynatan H AUID- ORCID: 0000-0002-3671-6540 AD - Department of Medical Oncology, Izmir City Hospital, Izmir 35540, Turkey. FAU - Ozdemir, Ozlem AU - Ozdemir O AUID- ORCID: 0000-0003-2520-5953 AD - Department of Medical Oncology, Izmir City Hospital, Izmir 35540, Turkey. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702203 OTO - NOTNLM OT - disease-free survival OT - gastric cancer OT - immunoscore OT - neoadjuvant chemotherapy OT - pathological response OT - tumor immune microenvironment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/15 CRDT- 2026/10/27 10:31 PHST- 2026/09/12 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/14 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - cancers18203329 [pii] AID - cancers-18-03329 [pii] AID - 10.3390/cancers18203329 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 15;18(20):3329. doi: 10.3390/cancers18203329. PMID- 42938862 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 15 TI - Urinary Biomarkers in Cancer: A Scoping Review. LID - 10.3390/cancers18203328 [doi] LID - 3328 AB - Cancer remains a major global health challenge and a leading cause of mortality worldwide. As incidence continues to rise, early detection remains one of the most effective strategies for improving survival and reducing disease burden. Urine is an attractive source of biomarkers due to its non-invasive collection, ease of repeated sampling, and rich molecular composition. This scoping review evaluates the current landscape of urinary biomarkers for the early detection of cancer and their potential for clinical translation. A detailed literature search was conducted using PubMed, Embase, and Web of Science to identify studies investigating urinary biomarkers for cancer detection. Biomarkers were categorised by analyte class, and diagnostic performance, validation status, and clinical applicability were assessed. Urinary biomarkers demonstrated diagnostic potential across multiple malignancies, including lung, breast, pancreatic, hepatobiliary, colorectal, renal and ovarian cancers, while several urinary assays are already clinically available for bladder and prostate cancers. Promising diagnostic accuracy was reported for multiple biomarker classes, particularly multimarker panels integrating metabolites, proteins, nucleic acids, and epigenetic signatures. However, most studies were exploratory, involving small cohorts, methodological heterogeneity, and limited independent validation. Urinary biomarkers represent a promising and rapidly evolving approach to non-invasive cancer detection. Despite encouraging diagnostic performance, few candidates have progressed to clinical implementation. Large prospective multicentre studies, rigorous validation, and assay standardisation are essential to establish urinary biomarkers as reliable tools for cancer screening, early diagnosis, and risk stratification. FAU - Kurotova, Evelyn AU - Kurotova E AD - Centre for Cancer Biomarkers and Biotherapeutics, Barts Cancer Institute, Queen Mary University of London, London EC1M 6BQ, UK. FAU - Shah, Jayni AU - Shah J AUID- ORCID: 0009-0003-3321-9762 AD - Centre for Cancer Biomarkers and Biotherapeutics, Barts Cancer Institute, Queen Mary University of London, London EC1M 6BQ, UK. FAU - Pueyo Valles, Lucia AU - Pueyo Valles L AD - Centre for Cancer Biomarkers and Biotherapeutics, Barts Cancer Institute, Queen Mary University of London, London EC1M 6BQ, UK. FAU - Burke, Edwina AU - Burke E AD - Centre for Cancer Biomarkers and Biotherapeutics, Barts Cancer Institute, Queen Mary University of London, London EC1M 6BQ, UK. FAU - Berreby, Ruben AU - Berreby R AD - Centre for Cancer Biomarkers and Biotherapeutics, Barts Cancer Institute, Queen Mary University of London, London EC1M 6BQ, UK. FAU - Lu, Yong-Jie AU - Lu YJ AUID- ORCID: 0000-0001-6174-6621 AD - Centre for Cancer Biomarkers and Biotherapeutics, Barts Cancer Institute, Queen Mary University of London, London EC1M 6BQ, UK. FAU - Debernardi, Silvana AU - Debernardi S AUID- ORCID: 0000-0003-3286-8632 AD - Centre for Cancer Biomarkers and Biotherapeutics, Barts Cancer Institute, Queen Mary University of London, London EC1M 6BQ, UK. FAU - Crnogorac-Jurcevic, Tatjana AU - Crnogorac-Jurcevic T AUID- ORCID: 0000-0002-2465-5605 AD - Centre for Cancer Biomarkers and Biotherapeutics, Barts Cancer Institute, Queen Mary University of London, London EC1M 6BQ, UK. LA - eng PT - Journal Article PT - Review DEP - 20261015 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702443 OTO - NOTNLM OT - biomarkers OT - cancer OT - urine COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/15 CRDT- 2026/10/27 10:31 PHST- 2026/08/29 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - cancers18203328 [pii] AID - cancers-18-03328 [pii] AID - 10.3390/cancers18203328 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 15;18(20):3328. doi: 10.3390/cancers18203328. PMID- 42938861 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 15 TI - Incremental Value and Cross-Center Validation of Pretreatment Tri-Modal Ultrasound Deep Learning for Predicting Breast Pathologic Complete Response to Neoadjuvant Chemotherapy: A Multicenter Study. LID - 10.3390/cancers18203326 [doi] LID - 3326 AB - Objective: To evaluate the incremental predictive value of pretreatment tri-modal ultrasound deep learning integrating two-dimensional ultrasound (2DUS), color Doppler flow imaging (CDFI), and contrast-enhanced ultrasound (CEUS) for predicting breast pathologic complete response (bpCR) after neoadjuvant chemotherapy (NAC), and to assess performance stability across independent centers. Methods: This retrospective multicenter study included 1089 patients with primary invasive breast cancer. Center 1 provided the training cohort (n = 572) and internal validation cohort (n = 246), whereas centers 2 (n = 160) and 3 (n = 111) served as independent external validation cohorts. Using pretreatment 2DUS, CDFI, and CEUS within a standardized intratumoral and 10-mm peritumoral framework, single-modality, all three dual-modality combinations, and tri-modal models were developed. The best-performing dual-modality model was identified and locked during training as the primary imaging comparator. A Tri-modal + Clinical model further integrated pretreatment clinical and biomarker variables. Performance was assessed using AUC, Brier score, calibration, decision curve analysis, and ablation experiments. Results: Overall, 383 patients (35.2%) achieved bpCR. CEUS was the strongest single modality, and 2DUS + CEUS was the best-performing dual-modality model. Compared with 2DUS + CEUS, the tri-modal model increased the AUC from 0.895 to 0.919 in the combined external cohort (DeltaAUC, +0.023; 95% CI, +0.010 to +0.038; p = 0.001), with corresponding improvements in Brier score (0.124 to 0.105) and F1 score (0.747 to 0.786). The AUC point estimate increased in both external centers, although the 95% CI for the incremental AUC included zero in external validation cohort 2. Despite modest standalone discrimination, CDFI provided measurable incremental information when added to 2DUS + CEUS. Further integration of pretreatment clinical and biomarker variables increased the combined external AUC to 0.935. Reduced-input retraining showed performance decreases after omission of any modality, with the largest reduction after CEUS omission. Conclusions: Pretreatment tri-modal ultrasound deep learning provided incremental predictive value for bpCR beyond the best dual-modality model, with a consistent direction of benefit across two independent external centers. Clinical and biomarker integration provided additional predictive information, supporting further evaluation of this multimodal framework for individualized pretreatment bpCR prediction. FAU - Wei, Chuanmin AU - Wei C AUID- ORCID: 0009-0003-4665-769X AD - Ultrasound Medical Center, The Second Hospital of Lanzhou University, Lanzhou 730030, China. FAU - Pan, Ting AU - Pan T AD - Department of Ultrasound Diagnosis, Gansu Provincial Maternity and Child-Care Hospital (Gansu Provincial Central Hospital), Lanzhou 730050, China. FAU - Li, Tiangang AU - Li T AD - Department of Ultrasound Diagnosis, Gansu Provincial Maternity and Child-Care Hospital (Gansu Provincial Central Hospital), Lanzhou 730050, China. FAU - Zheng, Chunyao AU - Zheng C AD - Ultrasound Medical Center, The Second Hospital of Lanzhou University, Lanzhou 730030, China. FAU - Duan, Ying AU - Duan Y AD - Department of Ultrasound, Gansu Provincial Cancer Hospital, Lanzhou 730050, China. FAU - Nie, Fang AU - Nie F AUID- ORCID: 0000-0001-9725-1743 AD - Ultrasound Medical Center, The Second Hospital of Lanzhou University, Lanzhou 730030, China. LA - eng GR - 8246070006/National Natural Science Foundation of China/ GR - 82560349/National Natural Science Foundation of China/ GR - 25JRRA599/Natural Science Foundation of Gansu Province/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702602 OTO - NOTNLM OT - breast cancer OT - contrast-enhanced ultrasound OT - deep learning OT - multimodal ultrasound OT - neoadjuvant chemotherapy OT - pathologic complete response COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/15 CRDT- 2026/10/27 10:31 PHST- 2026/10/01 00:00 [received] PHST- 2026/10/11 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - cancers18203326 [pii] AID - cancers-18-03326 [pii] AID - 10.3390/cancers18203326 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 15;18(20):3326. doi: 10.3390/cancers18203326. PMID- 42938858 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 15 TI - Personalized Prediction of Cutaneous Melanoma Risk Based on Polymorphism of the Genes Related to Skin Aging, Phenotypic Characteristics, and Sun Exposure in Lithuania. LID - 10.3390/cancers18203323 [doi] LID - 3323 AB - Background/Objectives: This is the first study in Eastern Europe to integrate skin and iris phenotypic characteristics with selected polymorphisms in genes related to skin aging, melanogenesis and skin pigmentation, and inflammatory regulation in relation to cutaneous melanoma (CM) risk. Methods: This case-control study included 180 patients with primary CM identified from the Hospital of the LSMU Kauno klinikos database and 181 controls without a personal history of skin cancer selected from a population-based study in Kaunas. All participants underwent dermatological and ophthalmological examinations and completed questionnaires assessing sun-exposure habits, including severe sunburn history, and Fitzpatrick skin phototype. The total-body number of common melanocytic nevi (CMN) >2 mm in diameter and the number of iris freckles were evaluated using standardized protocols. Five selected genetic polymorphisms, including TYR rs1126809, SLC45A2 rs26722, SLC45A2 rs16891982, IL6 rs1800795, and TNF-alpha rs1800629, were genotyped. Associations with CM were assessed using multivariable logistic regression. Results: We identified three phenotypical factors independently associated with increased odds of CM (OR; 95% CI): a total-body CMN count >15 (7.89; 4.29-14.53), >/=4 iris freckles (3.70; 2.04-6.74), and a history of severe sunburn (2.76; 1.50-5.07). Compared with Fitzpatrick skin phototype IV, phototypes II (0.35; 0.15-0.82) and III (0.28; 0.11-0.70) were associated with decreased CM odds. In the multivariable model the heterozygous SLC45A2 rs16891982 genotype was associated with increased CM odds (6.64; 1.48-29.83), while the IL6 rs1800795 Mono G genotype revealed an inverse association with CM (0.39; 0.16-0.97). Conclusions: A total-body CMN count >15, >/=4 iris freckles, and severe sunburn history revealed strong independent associations with increased CM risk. However, associations involving SLC45A2 rs16891982 and IL6 rs1800795 in this Lithuanian case-control study were model-dependent. These findings highlight the potential value of integrating selected genetic markers with phenotypic characteristics and sun-exposure history in the development of personalized CM risk prediction models; however, validation in larger populations is required. FAU - Butrimas, Grinvydas AU - Butrimas G AD - Department of Skin and Venereal Diseases, Medical Academy, Lithuanian University of Health Sciences (LSMU), LT-50161 Kaunas, Lithuania. FAU - Semeklis, Lukas AU - Semeklis L AUID- ORCID: 0000-0001-7001-1782 AD - Department of Ophthalmology, Medical Academy, Lithuanian University of Health Sciences (LSMU), LT-50161 Kaunas, Lithuania. FAU - Paukstaitiene, Renata AU - Paukstaitiene R AUID- ORCID: 0000-0002-3827-0984 AD - Department of Physics, Mathematics and Biophysics, Medical Academy, Lithuanian University of Health Sciences (LSMU), LT-44307 Kaunas, Lithuania. FAU - Oguleviciute, Aiste AU - Oguleviciute A AD - Department of Skin and Venereal Diseases, Medical Academy, Lithuanian University of Health Sciences (LSMU), LT-50161 Kaunas, Lithuania. FAU - Miseikyte, Gaile AU - Miseikyte G AUID- ORCID: 0009-0001-4640-6298 AD - Faculty of Medicine, Medical Academy, Lithuanian University of Health Sciences (LSMU), LT-44307 Kaunas, Lithuania. FAU - Luksiene, Dalia AU - Luksiene D AD - Laboratory of Population Studies, Institute of Cardiology, Lithuanian University of Health Sciences (LSMU), LT-50103 Kaunas, Lithuania. FAU - Valiukeviciene, Skaidra AU - Valiukeviciene S AD - Department of Skin and Venereal Diseases, Medical Academy, Lithuanian University of Health Sciences (LSMU), LT-50161 Kaunas, Lithuania. LA - eng GR - 02-020-K-0071/Lithuanian Innovation Agency and UAB "Medicina Practica Laboratorija"/ PT - Journal Article DEP - 20261015 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702231 OTO - NOTNLM OT - cutaneous melanoma OT - phenotypic characteristics of skin and iris OT - risk factors OT - skin-aging polymorphisms COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/15 CRDT- 2026/10/27 10:31 PHST- 2026/09/11 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/13 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - cancers18203323 [pii] AID - cancers-18-03323 [pii] AID - 10.3390/cancers18203323 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 15;18(20):3323. doi: 10.3390/cancers18203323. PMID- 42938857 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 15 TI - Is HER2-Low Breast Cancer a Distinct Prognostic Entity? Real-World Survival Outcomes from a Cohort in Colombia. LID - 10.3390/cancers18203322 [doi] LID - 3322 AB - Background/Objectives: The HER2-low classification has redefined therapeutic strategies, but its prognostic significance remains uncertain. We compared overall survival (OS) and progression-free survival (PFS) across HER2 categories (HER2-low, HER2-negative, HER2-positive) in a real-world cohort. Methods: This was a single-center retrospective cohort study conducted at a high-complexity cancer center in Colombia; it included 373 patients with invasive breast cancer classified as HER2-positive (IHC 3+ or ISH+), HER2-low (IHC 1+ or 2+/ISH-), or HER2-negative (IHC 0). Survival was estimated by Kaplan-Meier. Multivariable Cox models were adjusted for age, clinical stage, histological grade, and hormone receptor status, and PFS was additionally analyzed separately in stage I-III and stage IV disease. Results: The cohort included HER2-low (38.3%), HER2-negative (39.7%), and HER2-positive (22.0%) tumors. HER2-low tumors showed lower grade and higher hormone receptor expression (p < 0.001), without better survival. Three-year OS (81.8%, 76.5%, 62.8%; p = 0.178) and PFS (p = 0.227) did not differ. Advanced stage remained the main determinant of mortality, whereas the association with high grade was attenuated. HER2-low was not independently associated with OS (HR 0.67; 95% CI 0.31-1.45) or PFS (HR 0.77; 95% CI 0.40-1.49). Hormone receptor positivity was associated with lower mortality (HR 0.43; 95% CI 0.21-0.88). PFS did not differ across HER2 categories in stage I-III (p = 0.307) or stage IV disease (p = 0.068). Conclusions: HER2-low status was not independently associated with survival. Its more favorable clinicopathological profile was largely explained by higher hormone receptor expression, which was itself associated with survival once included in the models. FAU - Manrique-Hernandez, Edgar Fabian AU - Manrique-Hernandez EF AD - Epidemiology Department, Hospital Internacional de Colombia (HIC), Fundacion Cardiovascular de Colombia (FCV), Fundacion Universitaria FCV, Bucaramanga 680001, Colombia. FAU - Licht-Ardila, Maricel AU - Licht-Ardila M AD - Epidemiology Department, Hospital Internacional de Colombia (HIC), Fundacion Cardiovascular de Colombia (FCV), Fundacion Universitaria FCV, Bucaramanga 680001, Colombia. FAU - Torres Rosas, Johan Danilo AU - Torres Rosas JD AUID- ORCID: 0000-0003-3931-7753 AD - Oncology Department, Hospital Internacional de Colombia (HIC), Fundacion Cardiovascular de Colombia (FCV), Fundacion Universitaria FCV, Bucaramanga 680001, Colombia. FAU - Rincon Pallares, Jorge Andres AU - Rincon Pallares JA AUID- ORCID: 0009-0000-7378-3681 AD - Oncology Department, Hospital Internacional de Colombia (HIC), Fundacion Cardiovascular de Colombia (FCV), Fundacion Universitaria FCV, Bucaramanga 680001, Colombia. FAU - Villabona Diaz, Michael Steven AU - Villabona Diaz MS AUID- ORCID: 0009-0002-7692-6806 AD - Oncology Department, Hospital Internacional de Colombia (HIC), Fundacion Cardiovascular de Colombia (FCV), Fundacion Universitaria FCV, Bucaramanga 680001, Colombia. FAU - Aldana Galvis, Lady Katerine AU - Aldana Galvis LK AUID- ORCID: 0009-0006-6304-0087 AD - Oncology Department, Hospital Internacional de Colombia (HIC), Fundacion Cardiovascular de Colombia (FCV), Fundacion Universitaria FCV, Bucaramanga 680001, Colombia. FAU - Hoyos, Kendrys AU - Hoyos K AD - Oncology Department, Hospital Internacional de Colombia (HIC), Fundacion Cardiovascular de Colombia (FCV), Fundacion Universitaria FCV, Bucaramanga 680001, Colombia. FAU - Hurtado-Ortiz, Alexandra AU - Hurtado-Ortiz A AUID- ORCID: 0000-0002-3001-2374 AD - Epidemiology Department, Hospital Internacional de Colombia (HIC), Fundacion Cardiovascular de Colombia (FCV), Fundacion Universitaria FCV, Bucaramanga 680001, Colombia. FAU - Calderon Cortes, Carlos AU - Calderon Cortes C AD - Oncology Department, Hospital Internacional de Colombia (HIC), Fundacion Cardiovascular de Colombia (FCV), Fundacion Universitaria FCV, Bucaramanga 680001, Colombia. FAU - Gomez-Abreo, Diego AU - Gomez-Abreo D AUID- ORCID: 0000-0002-4376-4583 AD - Oncology Department, Hospital Internacional de Colombia (HIC), Fundacion Cardiovascular de Colombia (FCV), Fundacion Universitaria FCV, Bucaramanga 680001, Colombia. LA - eng PT - Journal Article DEP - 20261015 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702211 OTO - NOTNLM OT - HER2 protein OT - breast neoplasms OT - cohort studies OT - disease-free survival OT - neoplasm staging OT - prognosis OT - survival analysis COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/15 CRDT- 2026/10/27 10:31 PHST- 2026/08/19 00:00 [received] PHST- 2026/09/09 00:00 [revised] PHST- 2026/09/23 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/15 00:00 [pmc-release] AID - cancers18203322 [pii] AID - cancers-18-03322 [pii] AID - 10.3390/cancers18203322 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 15;18(20):3322. doi: 10.3390/cancers18203322. PMID- 42938854 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 14 TI - Advances in Interventional Oncology for Hepatocellular Carcinoma. LID - 10.3390/cancers18203319 [doi] LID - 3319 AB - Locoregional therapies are used across the hepatocellular carcinoma (HCC) treatment spectrum, including for curative treatment of small tumors, as bridging or downstaging therapy before liver transplant, for treatment of intermediate-stage disease, and as part of combination treatment with systemic therapy in selected patients. This narrative review summarizes recent developments in transarterial radioembolization (TARE), transarterial chemoembolization (TACE), and percutaneous ablation techniques and separates established clinical advances from early investigational work. The strongest evidence supports personalized TARE dosimetry and radiation segmentectomy, selective TACE with reassessment before repeat treatment, and image-guided thermal ablation with objective margin assessment. Phase 3 trials of TACE combined with antiangiogenic and immune checkpoint therapy demonstrated improved progression-free survival in selected patients with unresectable, nonmetastatic HCC, although the survival benefit of these combinations so far extends only to progression-free survival, and the best treatment sequence remains to be defined. Histotripsy, pulsed electric-field ablation, electrochemotherapy, cryoablation, high-intensity focused ultrasound, imageable or alternative radioembolic microspheres, new embolic agents, robotics, and artificial intelligence remain at different stages of clinical development. Most supporting data are derived from single-arm studies, retrospective cohort studies, trials in mixed liver-tumor populations, or studies in preclinical models. Treatment selection should account for tumor burden and location, liver function, portal hypertension, vascular invasion, transplant candidacy, available alternatives, local expertise, and patient preferences. Future studies should focus on durable local control, preservation of liver function, quality of life, transplant eligibility, overall survival, and cost. FAU - Gadani, Sameer AU - Gadani S AD - Interventional Radiology Division, Imaging Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA. FAU - Chou, Edwin AU - Chou E AD - Interventional Radiology Division, Imaging Institute, Cleveland Clinic Foundation, Cleveland, OH 44195, USA. FAU - Chansangrat, Jirapa AU - Chansangrat J AUID- ORCID: 0000-0002-7004-1065 AD - School of Radiology, Institute of Medicine, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand. FAU - Salar, Hashim AU - Salar H AUID- ORCID: 0009-0008-9067-1620 AD - Medical College, Faculty of Health Sciences, Aga Khan University, Karachi 74800, Pakistan. FAU - Kapoor, Baljendra AU - Kapoor B AUID- ORCID: 0009-0001-2647-9596 AD - Department of Radiology, University of Michigan, Ann Arbor, MI 48109, USA. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702686 OTO - NOTNLM OT - combination therapy OT - hepatocellular carcinoma OT - immunotherapy OT - interventional oncology OT - locoregional therapy OT - percutaneous ablation OT - transarterial chemoembolization OT - transarterial radioembolization COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/14 CRDT- 2026/10/27 10:31 PHST- 2026/08/22 00:00 [received] PHST- 2026/09/20 00:00 [revised] PHST- 2026/10/10 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - cancers18203319 [pii] AID - cancers-18-03319 [pii] AID - 10.3390/cancers18203319 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 14;18(20):3319. doi: 10.3390/cancers18203319. PMID- 42938853 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 14 TI - Current Status, Clinical Outcomes and Socioeconomic Challenges of Uniportal Video-Assisted Thoracoscopic Surgery in Japan Compared with Robot-Assisted Thoracic Surgery: A Narrative Review. LID - 10.3390/cancers18203318 [doi] LID - 3318 AB - Multiportal video-assisted thoracoscopic surgery (VATS) was introduced in Japan in the 1990s, with uniportal VATS (U-VATS) and robot-assisted thoracic surgery (RATS) following in the 2010s. U-VATS gained significant traction after the 1st Fellowship of the Japanese Association for Chest Surgery (JACS; currently the Japanese Society for Thoracic Surgery, JSTS) in 2018, leading to the establishment of the Japanese Uniportal VATS Interest Group (JUVIG) in April 2018. JUVIG has since driven wider adoption through extensive activities, including educational programs, publications, and collaborative multicenter studies. As a result, expert surgeons now utilize U-VATS for complex procedures such as bronchoplasty, completion lobectomy, complex segmentectomy, and pediatric surgery. Concurrently, RATS gained insurance coverage in Japan in 2018 and has become more prevalent than U-VATS. Despite the significant costs associated with RATS, its adoption rate surpasses that of U-VATS due to institutional policies, perceived ease of implementation, branding, recruitment incentives, and recent national healthcare insurance reimbursement revisions (add-ons) introduced in 2026, which may create administrative pressure to prioritize robotic cases. Although reduced-port RATS is gaining popularity-blurring the lines with U-VATS-and newly emerging indigenous robotic platforms are expected to further accelerate RATS adoption, multiportal VATS (M-VATS) remains the dominant approach in many institutions. Furthermore, maintaining proficiency in VATS, U-VATS, and open thoracotomy remains indispensable for surgical education, economic viability, and acute intraoperative crisis management. Ultimately, while U-VATS represents a promising minimally invasive option, its widespread adoption for major and complex resections in Japan continues to be restricted by both educational and societal barriers. FAU - Homma, Takahiro AU - Homma T AUID- ORCID: 0000-0001-5117-5570 AD - Department of Thoracic Surgery, St. Marianna University School of Medicine, Kawasaki 216-8511, Japan. FAU - Awane, Shota AU - Awane S AD - Department of Thoracic Surgery, St. Marianna University School of Medicine, Kawasaki 216-8511, Japan. FAU - Tamura, Moeka AU - Tamura M AD - Department of Thoracic Surgery, St. Marianna University School of Medicine, Kawasaki 216-8511, Japan. FAU - Kakizaki, Norifumi AU - Kakizaki N AD - Department of Thoracic Surgery, St. Marianna University School of Medicine, Kawasaki 216-8511, Japan. FAU - Hatakeyama, Takayuki AU - Hatakeyama T AUID- ORCID: 0009-0001-0192-6855 AD - Department of Thoracic Surgery, St. Marianna University School of Medicine, Kawasaki 216-8511, Japan. FAU - Otsubo, Kanji AU - Otsubo K AD - Department of Thoracic Surgery, St. Marianna University School of Medicine, Kawasaki 216-8511, Japan. FAU - Sakai, Hiroki AU - Sakai H AD - Department of Thoracic Surgery, St. Marianna University School of Medicine, Kawasaki 216-8511, Japan. FAU - Marushima, Hideki AU - Marushima H AD - Department of Thoracic Surgery, St. Marianna University School of Medicine, Kawasaki 216-8511, Japan. FAU - Kojima, Koji AU - Kojima K AD - Department of Thoracic Surgery, St. Marianna University School of Medicine, Kawasaki 216-8511, Japan. FAU - Saji, Hisashi AU - Saji H AUID- ORCID: 0000-0003-3490-7658 AD - Department of Thoracic Surgery, St. Marianna University School of Medicine, Kawasaki 216-8511, Japan. LA - eng GR - JP25ck0106050/Japan Agency for Medical Research and Development/ PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702665 OTO - NOTNLM OT - minimally invasive surgery OT - robot-assisted thoracic surgery OT - uniportal OT - video-assisted thoracoscopic surgery COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/14 CRDT- 2026/10/27 10:31 PHST- 2026/09/03 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - cancers18203318 [pii] AID - cancers-18-03318 [pii] AID - 10.3390/cancers18203318 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 14;18(20):3318. doi: 10.3390/cancers18203318. PMID- 42938852 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 14 TI - BCIW-Net: Boundary-Conditioned Adaptive CT Windowing for Hepatocellular Carcinoma Segmentation. LID - 10.3390/cancers18203317 [doi] LID - 3317 AB - Background/Objectives: Hepatocellular carcinoma (HCC) is the most common primary liver malignancy, and accurate tumour segmentation in contrast-enhanced computed tomography is essential for treatment planning, assessment of ablation margins, and post-treatment evaluation. Existing deep learning methods typically use fixed-intensity CT preprocessing, overlooking the adaptive windowing employed by radiologists to enhance tumour visibility. To address this limitation, we propose BCIW-Net, a boundary-conditioned intensity-windowed network that integrates spatially adaptive and differentiable intensity windowing into the segmentation process as a clinically motivated inductive bias. Methods: The proposed framework first generates complementary soft-tissue and tumour-enhancing intensity representations using differentiable sigmoid-based transformations. A primary encoder-decoder module predicts tumour guidance, boundary probabilities, and pixel-wise error proxy maps, which collectively guide the dynamic estimation of spatially varying window centre and width parameters. The resulting adaptively windowed features are selectively blended with fixed-intensity representations through an error-gated mechanism before final refinement in a secondary encoder-decoder network. A multi-task objective jointly optimises tumour segmentation, boundary prediction, error-proxy estimation, and adaptive window regularisation in an end-to-end manner. Results: Experiments on the primary liver cancer (PLC)-CECT dataset demonstrate that BCIW-Net achieved a mean Dice similarity coefficient of 78.68% and an intersection over union (IoU) of 64.85% across five-fold patient-level cross-validation, outperforming the seven evaluated CNN-, Transformer-, and state-space baselines. Evaluation on LiTS2017 without dataset-specific fine-tuning yielded a tumour Dice score of 89.84%, providing preliminary evidence of cross-dataset generalisation. Conclusions: Qualitative feature visualisations show that adaptive windowing enhances tumour representation and boundary delineation, demonstrating the effectiveness and interpretability of BCIW-Net for HCC segmentation. FAU - Singh, Tripti AU - Singh T AD - Department of Computer Engineering and Mathematics, Universitat Rovira i Virgili, 43007 Tarragona, Spain. FAU - Kampel, Julia Rebecca AU - Kampel JR AD - Centre for Cancer Biomarkers & Biotherapeutics, Barts Cancer Institute, Queen Mary University of London, London EC1M 6BQ, UK. FAU - Akram, Farhan AU - Akram F AUID- ORCID: 0000-0003-4109-2645 AD - Department of Pathology and Clinical Bioinformatics, Erasmus MC, 3015 GD Rotterdam, The Netherlands. FAU - Li, Yifan AU - Li Y AD - Centre for Cancer Biomarkers & Biotherapeutics, Barts Cancer Institute, Queen Mary University of London, London EC1M 6BQ, UK. FAU - Tiwari, Akshat AU - Tiwari A AD - Centre for Cancer Biomarkers & Biotherapeutics, Barts Cancer Institute, Queen Mary University of London, London EC1M 6BQ, UK. FAU - Deshpande, Sheetal Santosh AU - Deshpande SS AD - Department of Computer Engineering and Mathematics, Universitat Rovira i Virgili, 43007 Tarragona, Spain. FAU - Bommaraveni, Shriya AU - Bommaraveni S AD - Queen's University Belfast, Belfast BT7 1NN, UK. FAU - Abdel-Nasser, Mohamed AU - Abdel-Nasser M AD - Electrical Engineering Department, Faculty of Engineering, Aswan University, Aswan 81528, Egypt. FAU - Puig, Domenec AU - Puig D AUID- ORCID: 0000-0002-0562-4205 AD - Department of Computer Engineering and Mathematics, Universitat Rovira i Virgili, 43007 Tarragona, Spain. FAU - Singh, Vivek Kumar AU - Singh VK AD - Centre for Cancer Biomarkers & Biotherapeutics, Barts Cancer Institute, Queen Mary University of London, London EC1M 6BQ, UK. LA - eng GR - G-003271/Barts Charity/ PT - Journal Article DEP - 20261014 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702670 OTO - NOTNLM OT - adaptive intensity windowing OT - boundary-aware learning OT - computed tomography OT - hepatocellular carcinoma OT - medical image analysis OT - tumour segmentation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/14 CRDT- 2026/10/27 10:31 PHST- 2026/09/08 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - cancers18203317 [pii] AID - cancers-18-03317 [pii] AID - 10.3390/cancers18203317 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 14;18(20):3317. doi: 10.3390/cancers18203317. PMID- 42938846 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 14 TI - Minimally Invasive Versus Open CRS/HIPEC for Peritoneal Surface Malignancies: Postoperative Recovery and Long-Term Outcomes-A Systematic Review and Meta-Analysis. LID - 10.3390/cancers18203310 [doi] LID - 3310 AB - Background: Minimally invasive surgery (MIS) is increasingly implemented for cytoreductive surgery (CRS) combined with hyperthermic intraperitoneal chemotherapy (HIPEC) for peritoneal surface malignancies (PSM). This systematic review and meta-analysis compared minimally invasive CRS/HIPEC (M-CRS/HIPEC) and open CRS/HIPEC (O-CRS/HIPEC) in terms of perioperative, postoperative, and long-term oncological outcomes. Methods: A systematic review and meta-analysis were conducted according to PRISMA guidelines. PubMed, Embase, Scopus, Web of Science, and the Cochrane Library were searched for comparative studies of M-CRS/HIPEC versus O-CRS/HIPEC in adult patients. Risk of bias was assessed using the Newcastle-Ottawa Scale and RoB 2.0. Pooled relative risks (RR) for dichotomous outcomes and standardized mean differences (SMD) for continuous outcomes were calculated with 95% confidence intervals (CI). Sensitivity analyses were performed to assess the potential influence of disease burden and primary tumor heterogeneity. Results: Twenty-one studies (1 randomized controlled trial and 20 retrospective cohorts) including 1184 patients (452 M-CRS/HIPEC and 732 O-CRS/HIPEC) were analyzed. Operative time did not differ between groups (SMD = 0.195; p = 0.667). Most cytoreductive procedures were comparable, although small bowel resections were less frequent with M-CRS/HIPEC (RR = 0.537; p = 0.018). The minimally invasive approach achieved higher CC0 rates (RR = 1.053; p = 0.045), with lower blood loss (SMD = -1.838; p < 0.001) and fewer transfusions (RR = 0.588; p = 0.002). Postoperative recovery favored M-CRS/HIPEC, with earlier return of bowel function and resumption of oral intake, shorter hospital stay, fewer readmissions, and earlier chemotherapy resumption (all p < 0.05). Overall and major morbidity were reduced, while postoperative mortality and 1-, 3-, and 5-year disease-free and overall survival did not differ significantly between groups. In the disease burden-restricted sensitivity analysis, differences in small bowel resection, CC0 rate, and 90-day readmission were no longer statistically significant, whereas the main perioperative and recovery benefits persisted. No significant differences in DFS or OS were identified in the additional sensitivity analyses at the evaluable time points. Conclusions: This meta-analysis supports a selective, expertise-driven use of M-CRS/HIPEC, offering perioperative and recovery advantages without significant differences in cytoreductive adequacy or long-term outcomes in appropriately selected patients. FAU - Aulicino, Matteo AU - Aulicino M AD - Pancreatic Surgery Unit, Fondazione Policlinico Agostino Gemelli IRCCS of Rome, 00168 Rome, Italy. FAU - Tondolo, Vincenzo AU - Tondolo V AD - Dipartimento di Medicina e Chirurgia Traslazionale, Universita Cattolica del Sacro Cuore di Roma, 00168 Rome, Italy. AD - General Surgery, Department of Medical and Surgical Sciences, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy. FAU - Abatini, Carlo AU - Abatini C AD - Dipartimento di Medicina e Chirurgia Traslazionale, Universita Cattolica del Sacro Cuore di Roma, 00168 Rome, Italy. AD - Surgical Unit of Peritoneum and Retroperitoneum, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Largo Agostino Gemelli 8, 00168 Rome, Italy. FAU - D'Annibale, Giorgio AU - D'Annibale G AUID- ORCID: 0009-0000-3513-8745 AD - Surgical Unit of Peritoneum and Retroperitoneum, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Largo Agostino Gemelli 8, 00168 Rome, Italy. FAU - Barberis, Lorenzo AU - Barberis L AUID- ORCID: 0009-0002-4885-4091 AD - Surgical Unit of Peritoneum and Retroperitoneum, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Largo Agostino Gemelli 8, 00168 Rome, Italy. FAU - Saiz, Maria AU - Saiz M AD - MEMOTEF Department, Sapienza University of Rome, 00161 Rome, Italy. FAU - Fiorillo, Claudio AU - Fiorillo C AD - Pancreatic Surgery Unit, Fondazione Policlinico Agostino Gemelli IRCCS of Rome, 00168 Rome, Italy. AD - Dipartimento di Medicina e Chirurgia Traslazionale, Universita Cattolica del Sacro Cuore di Roma, 00168 Rome, Italy. FAU - De Sio, Davide AU - De Sio D AD - Pancreatic Surgery Unit, Fondazione Policlinico Agostino Gemelli IRCCS of Rome, 00168 Rome, Italy. AD - Dipartimento di Medicina e Chirurgia Traslazionale, Universita Cattolica del Sacro Cuore di Roma, 00168 Rome, Italy. FAU - Quero, Giuseppe AU - Quero G AD - Pancreatic Surgery Unit, Fondazione Policlinico Agostino Gemelli IRCCS of Rome, 00168 Rome, Italy. AD - Dipartimento di Medicina e Chirurgia Traslazionale, Universita Cattolica del Sacro Cuore di Roma, 00168 Rome, Italy. FAU - Alfieri, Sergio AU - Alfieri S AD - Pancreatic Surgery Unit, Fondazione Policlinico Agostino Gemelli IRCCS of Rome, 00168 Rome, Italy. AD - Dipartimento di Medicina e Chirurgia Traslazionale, Universita Cattolica del Sacro Cuore di Roma, 00168 Rome, Italy. FAU - Pacelli, Fabio AU - Pacelli F AD - Dipartimento di Medicina e Chirurgia Traslazionale, Universita Cattolica del Sacro Cuore di Roma, 00168 Rome, Italy. AD - Surgical Unit of Peritoneum and Retroperitoneum, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Largo Agostino Gemelli 8, 00168 Rome, Italy. FAU - Santullo, Francesco AU - Santullo F AD - Dipartimento di Medicina e Chirurgia Traslazionale, Universita Cattolica del Sacro Cuore di Roma, 00168 Rome, Italy. AD - Surgical Unit of Peritoneum and Retroperitoneum, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, Largo Agostino Gemelli 8, 00168 Rome, Italy. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702463 OTO - NOTNLM OT - cytoreductive surgery OT - hyperthermic intraperitoneal chemotherapy OT - meta-analysis OT - minimally invasive surgery OT - peritoneal surface malignancies OT - systematic review COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/14 CRDT- 2026/10/27 10:31 PHST- 2026/08/29 00:00 [received] PHST- 2026/10/05 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - cancers18203310 [pii] AID - cancers-18-03310 [pii] AID - 10.3390/cancers18203310 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 14;18(20):3310. doi: 10.3390/cancers18203310. PMID- 42938845 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 14 TI - Is the MRI Phenotype Associated with Survival in Patients with Different Breast Cancer Subtypes? LID - 10.3390/cancers18203309 [doi] LID - 3309 AB - BACKGROUND: Breast cancer exhibits marked molecular and phenotypic heterogeneity, with important implications for prognosis and therapeutic response. Although magnetic resonance imaging (MRI) is widely used for detection and staging, its potential role as a prognostic biomarker across molecular subtypes remains incompletely understood. This study aimed to evaluate the association between MRI-defined tumor imaging phenotypes and disease-free survival (DFS) among different breast cancer molecular subtypes. METHODS: This retrospective single-center study included 407 patients with invasive breast carcinoma of no special type who underwent pre-treatment breast MRI between October 2015 and May 2021. Tumors were classified into molecular subtypes and three MRI phenotypes based on lesion morphology and distribution. Associations between imaging phenotype, molecular subtype, and DFS were assessed using Kaplan-Meier analysis and Cox proportional hazards models. RESULTS: Type 3 was the most prevalent imaging phenotype overall (60%), followed by Type 2 (21.9%) and Type 1 (18.2%). Imaging phenotype distribution differed significantly among molecular subtypes. Over a median follow-up of 57 months, imaging phenotype was not associated with DFS in Luminal A, Luminal B, or HER2-overexpressed tumors. However, in the triple-negative/ER-low subgroup, imaging phenotype demonstrated an independent association with DFS. In multivariable analysis, patients with the Type 1 phenotype had an 8.16-fold higher risk of recurrence compared with those with the Type 2 phenotype (95% CI: 1.49-44.60; p = 0.015). Type 2 lesions showed the most favorable survival outcomes. CONCLUSIONS: In this exploratory analysis, MRI phenotype was associated with disease-free survival in triple-negative and ER-low breast cancers. Circumscribed, unifocal tumors are associated with improved survival, whereas spiculated or multifocal patterns are associated with worse outcomes. However, given the small number of events in this subgroup, these exploratory findings need to be confirmed in larger studies before MRI morphology can be considered a prognostic imaging biomarker in aggressive breast cancer subtypes. FAU - Tajima, Carla Chizuru AU - Tajima CC AD - Department of Imaging, Beneficencia Portuguesa de Sao Paulo, Sao Paulo 01323-001, Brazil. AD - Graduate Program, A.C. Camargo Cancer Center, Sao Paulo 01509-010, Brazil. FAU - Damiao, Soraia Quaranta AU - Damiao SQ AD - Department of Imaging, A.C. Camargo Cancer Center, Sao Paulo 01509-010, Brazil. FAU - Osorio, Cynthia Aparecida Bueno de Toledo AU - Osorio CABT AD - Department of Pathology, A.C. Camargo Cancer Center, Sao Paulo 01509-010, Brazil. FAU - Sonagli, Marina AU - Sonagli M AD - Department of Breast Surgery, A.C. Camargo Cancer Center, Sao Paulo 01509-010, Brazil. FAU - Sanches, Solange Moraes AU - Sanches SM AD - Department of Clinical Oncology, A.C. Camargo Cancer Center, Sao Paulo 01509-010, Brazil. FAU - Bitencourt, Almir Galvao Vieira AU - Bitencourt AGV AUID- ORCID: 0000-0003-0192-9885 AD - Department of Imaging, A.C. Camargo Cancer Center, Sao Paulo 01509-010, Brazil. LA - eng PT - Journal Article DEP - 20261014 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702313 OTO - NOTNLM OT - breast neoplasms OT - disease-free survival OT - magnetic resonance imaging OT - phenotype OT - prognosis OT - triple-negative breast neoplasms COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/14 CRDT- 2026/10/27 10:31 PHST- 2026/08/06 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - cancers18203309 [pii] AID - cancers-18-03309 [pii] AID - 10.3390/cancers18203309 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 14;18(20):3309. doi: 10.3390/cancers18203309. PMID- 42938841 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 14 TI - Head-to-Head Comparison Between [(18)F]FDG and [(68)Ga]Ga-Pentixafor PET/CT in Multiple Myeloma: A Systematic Review and Meta-Analysis. LID - 10.3390/cancers18203305 [doi] LID - 3305 AB - Background/objective: Multiple myeloma (MM) is a hematological malignancy characterized by the proliferation of clonal plasma cells. While [(18)F]FDG PET/CT is widely established for assessing disease extent, bone involvement, and treatment response in MM, novel molecular imaging targets like CXCR4 (e.g., using [(68)Ga]Ga-Pentixafor) have emerged to offer potentially superior sensitivity and theranostic applications. The aim of this manuscript is to systematically review and compare the diagnostic performance, expressed as detection rates, of CXCR4-targeted PET/CT versus [(18)F]FDG PET/CT in patients with MM. Methods: A systematic literature search was conducted across major databases (PubMed/MEDLINE, Embase, and the Cochrane Library) for head-to-head comparative studies evaluating both [(18)F]FDG and [(68)Ga]Ga-Pentixafor PET/CT in MM patients. Risk of bias and study quality were assessed using the QUADAS-2 tool. Results: Eleven studies were collected, amounting to a total of 347 patients. A patient-based analysis showed that [(68)Ga]Ga-Pentixafor had a higher detection rate than [(18)F]FDG PET/CT (86.3%, 95% CI: 80.3-92.3% vs 60.9%, 95% CI: 42.5-79.4%). In a descriptive analysis, mean lesion SUVmax values reported across individual studies were generally higher with [(68)Ga]Ga-Pentixafor PET/CT than with [(18)F]FDG PET/CT. Conclusions: [(68)Ga]Ga-Pentixafor PET/CT may serve as a robust alternative or complementary imaging modality to [(18)F]FDG PET/CT in MM. It could also be helpful for selection in CXCR4-directed theranostic pathways, but more high-quality studies are warranted. FAU - Albano, Domenico AU - Albano D AUID- ORCID: 0000-0003-0810-6494 AD - Nuclear Medicine, University of Brescia, 25121 Brescia, Italy. AD - Nuclear Medicine Department, ASST Spedali Civili di Brescia, 25123 Brescia, Italy. FAU - Tasevski, Slavko AU - Tasevski S AUID- ORCID: 0000-0001-7847-2778 AD - Nuclear Medicine, University Institute for Positron Emission Tomography, 1000 Skopje, North Macedonia. FAU - Rapa, Marco AU - Rapa M AUID- ORCID: 0009-0000-7726-0255 AD - Department of Experimental, Diagnostic and Specialty Medicine, Alma Mater Studiorum, University of Bologna, 40138 Bologna, Italy. FAU - Nanni, Cristina AU - Nanni C AD - Nuclear Medicine Unit, Istituto di Ricovero e Cure a Carattere Scientifico (IRCCS), Azienda Ospedaliero-Universitaria di Bologna, 40138 Bologna, Italy. FAU - Treglia, Giorgio AU - Treglia G AUID- ORCID: 0000-0001-9808-780X AD - Faculty of Biology and Medicine, University of Lausanne, 1015 Lausanne, Switzerland. AD - Division of Nuclear Medicine, Ente Ospedaliero Cantonale, 6500 Bellinzona, Switzerland. AD - Faculty of Biomedical Sciences, Universita della Svizzera Italiana, 6900 Lugano, Switzerland. LA - eng PT - Journal Article PT - Review DEP - 20261014 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702103 OTO - NOTNLM OT - CXCR4 OT - FDG OT - PET OT - PET/CT OT - meta-analysis OT - multiple myeloma OT - pentixafor COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/14 CRDT- 2026/10/27 10:31 PHST- 2026/09/17 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/14 00:00 [pmc-release] AID - cancers18203305 [pii] AID - cancers-18-03305 [pii] AID - 10.3390/cancers18203305 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 14;18(20):3305. doi: 10.3390/cancers18203305. PMID- 42938837 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 13 TI - Alternative Isoforms as Molecular Modulators of Cellular Senescence in Cancer. LID - 10.3390/cancers18203301 [doi] LID - 3301 AB - Cellular senescence is a stress-induced, stable proliferative arrest that acts as a potent barrier to tumorigenesis, yet the senescence-associated secretory phenotype that accompanies it can paradoxically fuel inflammation, angiogenesis and the phenotypic plasticity contributing to tumor heterogeneity, therapy resistance and cancer stem cell emergence. A largely unexplored molecular layer in the senescence program is alternative pre-mRNA splicing, which generates protein isoforms with functionally divergent effects on cell-cycle arrest, apoptosis and the secretory program. More than 90% of human multi-exon genes are alternatively spliced, so most of the nearly 1000 genes linked to senescence are expected to produce several isoforms, yet fewer than twenty have been examined at this level, and direct evidence is available for only about half of these. We review how alternative splicing, together with related mechanisms such as alternative promoter usage and alternative reading-frame selection, modulates distinct outputs of the senescence program, including commitment, persistence, escape and SASP composition, across gene groups such as the TP53/TP63/TP73 family, MDM2/MDM4 and the androgen receptor locus. This evidence ranges from direct causal manipulation to correlative or mechanistically adjacent findings, classified explicitly by evidence level. Direct causal evidence remains limited, making isoform-resolved functional validation a major unmet need in the field. FAU - Garcia-Heredia, Jose Manuel AU - Garcia-Heredia JM AUID- ORCID: 0000-0003-3095-0050 AD - Departamento de Bioquimica Vegetal y Biologia Molecular, Universidad de Sevilla, 41012 Sevilla, Spain. AD - Instituto de Biomedicina de Sevilla (IBIS), Hospital Universitario Virgen del Rocio (HUVR), Consejo Superior de Investigaciones Cientificas (CSIC), Universidad de Sevilla, 41013 Sevilla, Spain. AD - CIBER de Cancer (CIBERONC), Instituto de Salud Carlos III, 28029 Madrid, Spain. LA - eng PT - Journal Article PT - Review DEP - 20261013 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702666 OTO - NOTNLM OT - SASP OT - alternative splicing OT - cell plasticity OT - cellular senescence OT - protein isoforms OT - therapy resistance OT - tumor microenvironment OT - tumor suppression COIS- The author declares no conflict of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/13 CRDT- 2026/10/27 10:31 PHST- 2026/10/02 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - cancers18203301 [pii] AID - cancers-18-03301 [pii] AID - 10.3390/cancers18203301 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 13;18(20):3301. doi: 10.3390/cancers18203301. PMID- 42938832 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 13 TI - Orlistat Suppresses Small Intestinal Neuroendocrine Tumor Growth Through ATF3-Associated Stress and Ferroptosis-Related Responses-In Vitro and Cell Line Xenograft Evidence. LID - 10.3390/cancers18203294 [doi] LID - 3294 AB - Background/Objectives: The incidence of small intestinal neuroendocrine tumors (SI-NETs) has increased in recent years, while effective therapeutic options remain limited. This study aimed to investigate the antitumor effects of orlistat on SI-NETs and elucidate the underlying mechanisms. Methods: The effects of orlistat were evaluated using STC-1 cells, patient-derived primary SI-NET cells, and xenograft models. Functional assays, RNA sequencing, lipidomic profiling, ATAC sequencing, and molecular analyses were performed to assess tumor-related phenotypes and investigate the underlying mechanisms. Results: Orlistat inhibited cell proliferation, migration, and invasion, induced G1-phase arrest and apoptosis, and suppressed tumor growth in vivo. Mechanistically, orlistat triggered endoplasmic reticulum stress, inhibited PI3K/AKT/mTOR signaling, and upregulated activating transcription factor 3 (ATF3). Lipidomic profiling revealed alterations in lipid metabolism. Increased reactive oxygen species and lipid peroxidation, together with changes in ferroptosis-related proteins, indicated the involvement of ferroptosis-associated pathways. ATF3 overexpression reproduced the antitumor and ferroptosis-associated effects of orlistat. Integrated ATAC-seq and RNA-seq analyses identified CHAC1 as a potential downstream effector of ATF3. Conversely, ATF3 knockdown reduced CHAC1 expression and attenuated the effects of orlistat both in vitro and in vivo. Conclusions: Orlistat suppresses SI-NET progression and is associated with changes in endoplasmic reticulum stress, ATF3 expression, and ferroptosis-related responses, with CHAC1 identified as a potential downstream effector of ATF3. These findings support further investigation of orlistat as a potential therapeutic strategy for SI-NETs. FAU - Zeng, Xiangrui AU - Zeng X AD - Department of Neuroendocrine Tumor, The First Affiliated Hospital with Nanjing Medical University, Neuroendocrine Tumor Diagnosis and Treatment Center of Jiangsu Province, Institute of Neuroendocrine Tumor of Nanjing Medical University, Nanjing 210029, China. FAU - Chen, Sixu AU - Chen S AD - Department of Neuroendocrine Tumor, The First Affiliated Hospital with Nanjing Medical University, Neuroendocrine Tumor Diagnosis and Treatment Center of Jiangsu Province, Institute of Neuroendocrine Tumor of Nanjing Medical University, Nanjing 210029, China. FAU - Long, Qin AU - Long Q AD - Department of Neuroendocrine Tumor, The First Affiliated Hospital with Nanjing Medical University, Neuroendocrine Tumor Diagnosis and Treatment Center of Jiangsu Province, Institute of Neuroendocrine Tumor of Nanjing Medical University, Nanjing 210029, China. FAU - Han, Xu AU - Han X AD - Department of Neuroendocrine Tumor, The First Affiliated Hospital with Nanjing Medical University, Neuroendocrine Tumor Diagnosis and Treatment Center of Jiangsu Province, Institute of Neuroendocrine Tumor of Nanjing Medical University, Nanjing 210029, China. FAU - Li, Weilong AU - Li W AD - Department of Neuroendocrine Tumor, The First Affiliated Hospital with Nanjing Medical University, Neuroendocrine Tumor Diagnosis and Treatment Center of Jiangsu Province, Institute of Neuroendocrine Tumor of Nanjing Medical University, Nanjing 210029, China. FAU - Ge, Jierong AU - Ge J AD - Department of Neuroendocrine Tumor, The First Affiliated Hospital with Nanjing Medical University, Neuroendocrine Tumor Diagnosis and Treatment Center of Jiangsu Province, Institute of Neuroendocrine Tumor of Nanjing Medical University, Nanjing 210029, China. FAU - Ding, Yi AU - Ding Y AD - Department of Neuroendocrine Tumor, The First Affiliated Hospital with Nanjing Medical University, Neuroendocrine Tumor Diagnosis and Treatment Center of Jiangsu Province, Institute of Neuroendocrine Tumor of Nanjing Medical University, Nanjing 210029, China. FAU - Ding, Yixuan AU - Ding Y AD - Department of Neuroendocrine Tumor, The First Affiliated Hospital with Nanjing Medical University, Neuroendocrine Tumor Diagnosis and Treatment Center of Jiangsu Province, Institute of Neuroendocrine Tumor of Nanjing Medical University, Nanjing 210029, China. FAU - Ye, Mujie AU - Ye M AD - Department of Neuroendocrine Tumor, The First Affiliated Hospital with Nanjing Medical University, Neuroendocrine Tumor Diagnosis and Treatment Center of Jiangsu Province, Institute of Neuroendocrine Tumor of Nanjing Medical University, Nanjing 210029, China. FAU - Tang, Qiyun AU - Tang Q AD - Department of Neuroendocrine Tumor, The First Affiliated Hospital with Nanjing Medical University, Neuroendocrine Tumor Diagnosis and Treatment Center of Jiangsu Province, Institute of Neuroendocrine Tumor of Nanjing Medical University, Nanjing 210029, China. LA - eng GR - Nos.82373180 and 82303453/National Natural Science Foundation of China/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702773 OTO - NOTNLM OT - CHAC1 OT - activating transcription factor 3 OT - endoplasmic reticulum stress OT - fatty acid synthase OT - ferroptosis OT - orlistat OT - small intestinal neuroendocrine tumors COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/13 CRDT- 2026/10/27 10:31 PHST- 2026/08/30 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - cancers18203294 [pii] AID - cancers-18-03294 [pii] AID - 10.3390/cancers18203294 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 13;18(20):3294. doi: 10.3390/cancers18203294. PMID- 42938831 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 13 TI - Real-World Utilization of Tissue-Free ctDNA MRD Testing in Non-Small Cell Lung Cancer Across Asia-Pacific and the Middle East: A Descriptive Cohort Analysis and Illustrative Case Series. LID - 10.3390/cancers18203296 [doi] LID - 3296 AB - Introduction: Circulating tumor DNA (ctDNA)-based molecular residual disease (MRD) testing is emerging as a tool to refine relapse risk and guide surveillance after curative-intent therapy for non-small cell lung cancer (NSCLC). Tissue-free (plasma-only) MRD assays may improve feasibility where tumor tissue is limited. Prospective and trial-embedded studies have established that ctDNA MRD detected after curative-intent therapy is prognostic for recurrence in NSCLC, but how tissue-free MRD testing is deployed in routine care in Asia-Pacific and the Middle East (AMEA) has not been described. We characterized ordering patterns and first-test positivity in an AMEA cohort. In this descriptive cohort (159 NSCLC patients; 257 samples), 40.9% of patients underwent more than one MRD test, with a median interval of 4.9 months between the first two tests; first-test MRD positivity was 15.7% (25/159; 95% CI 10.9-22.2) with rates of 0% (0/9; 95% CI 0.0-29.9), 8.8% (5/57; 95% CI 3.8-18.9), 21.5% (17/79; 95% CI 13.9-31.8) and 21.4% (3/14; 95% CI 7.6-47.6), respectively, across stages I, II, III, and oligometastatic stage IV disease. No hypothesis testing was performed, and the stage I and oligometastatic stage IV subgroups are small, so the confidence intervals overlap widely and apparent between-stage differences should not be interpreted as a stage effect. Case Presentation: Six illustrative cases involving patients with stage II-IVB describe the use of MRD testing in routine clinical care. The cases were selected purposively, not consecutively, to span the contexts in which the results were interpreted: a positive result shortly before radiographic nodal recurrence (Case 1); a positive result during long-term adjuvant osimertinib preceding isolated mediastinal relapse (Case 2); a transient positive result with subsequent clearance and radiographic regression (Case 3); a positive result that prompted PET-CT revealing occult metastatic disease (Case 4); serially negative results concordant with sustained radiographic remission (Case 5); and a positive result that prompted comprehensive genomic profiling identifying an actionable KRAS G12C alteration (Case 6). Conclusions: Across Asia-Pacific and the Middle East, tissue-free MRD testing in NSCLC was used primarily during surveillance and often serially; first-test positivity was 15.7% (95% CI 10.9-22.2) overall, with wide and mutually overlapping confidence intervals across stages. The cases illustrate that MRD results may prompt diagnostic evaluation when interpreted alongside imaging and other clinical findings, but they are purposively selected, and treatment changes that followed a result are reported as description, not as evidence of benefit. No assay performance characteristic is estimated. These findings are hypothesis-generating; prospective, MRD-directed trials with standardized timepoints, thresholds, confirmatory strategies and pre-specified clinical actions are needed before MRD results can guide treatment. FAU - Lee, Chun-Hui AU - Lee CH AUID- ORCID: 0000-0002-5276-9896 AD - Department of Oncology, National Cheng Kung University Hospital, Tainan 704, Taiwan. FAU - Jain, Suyog AU - Jain S AUID- ORCID: 0000-0002-2223-9181 AD - Medical Affairs Department, Guardant Health Pte Ltd., Singapore 138567, Singapore. FAU - Chen, Lun-Che AU - Chen LC AD - Department of Internal Medicine, National Taiwan University Hospital Hsinchu Branch, Hsinchu 300, Taiwan. FAU - Peled, Nir AU - Peled N AD - The Helmsley Cancer Center, Shaare Zedek Medical Center, Jerusalem 9103102, Israel. FAU - Cornelio, Gerardo H AU - Cornelio GH AD - Cancer Institute of St. Luke's Medical Center, Bonifacio Global City, Taguig 1634, Metro Manila, Philippines. FAU - Ng, Kenny Kim Pong AU - Ng KKP AD - Medical Oncology, reVIVE Oncology and Cancer Centre, Hong Kong, China. FAU - Jen, Hsiao-Yu AU - Jen HY AUID- ORCID: 0009-0007-5159-0150 AD - Medical Affairs Department, Guardant Health Pte Ltd., Singapore 138567, Singapore. FAU - Oh, Katherina AU - Oh K AUID- ORCID: 0009-0003-6817-6686 AD - Medical Affairs Department, Guardant Health Pte Ltd., Singapore 138567, Singapore. FAU - Castro-Maddela, Orielle Kyra B AU - Castro-Maddela OKB AD - St Luke's Medical Center, Quezon City 1112, Metro Manila, Philippines. FAU - Blanco, Vince Nigel AU - Blanco VN AD - St Luke's Medical Center, Quezon City 1112, Metro Manila, Philippines. FAU - Tsai, Chun-Ming AU - Tsai CM AD - Department of Oncology, Taipei Veterans General Hospital, Taipei City 112201, Taiwan. LA - eng PT - Journal Article DEP - 20261013 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702109 OTO - NOTNLM OT - Asia-Pacific OT - circulating tumor DNA OT - molecular residual disease OT - non-small cell lung cancer OT - surveillance COIS- L.-C.C., N.P., G.H.C., K.K.P.N., and C.-M.T. received honoraria from Guardant Health as speakers. S.J., K.O., and H-.Y.J. are employees of Guardant Health Pte. Ltd. and own shares in Guardant Health. C.-H.L., O.K.B.C.-M., and V.N.B. declare no conflicts of interest. The authors wish to state this relationship explicitly rather than by inference: three authors (S.J., K.O., and H.-Y.J.) are employees of Guardant Health Pte. Ltd. and hold equity in Guardant Health, Inc., the manufacturer of the Guardant Reveal assay that is the subject of this report; five further authors (L.-C.C., N.P., G.H.C., K.K.P.N., and C.-M.T.) have received speaker honoraria from the same company; and the work was funded by that company. The analysis concerns the funder's own commercially marketed assay, and the authors acknowledge that this constitutes a financial relationship with a direct potential to bias interpretation. Four measures were adopted to limit that potential: the analysis was restricted to descriptive utilization endpoints, with no comparative, outcome or benefit claim; no assay performance characteristic (sensitivity, specificity, or positive or negative predictive value) is reported, estimated or implied anywhere in the manuscript; all interpretive statements are framed as hypothesis-generating, and the investigational status of MRD-guided management is stated in the Abstract, Discussion and Conclusions; and the manuscript was reviewed and approved by all authors, including those with no financial relationship to the funder. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/13 CRDT- 2026/10/27 10:31 PHST- 2026/08/18 00:00 [received] PHST- 2026/09/24 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - cancers18203296 [pii] AID - cancers-18-03296 [pii] AID - 10.3390/cancers18203296 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 13;18(20):3296. doi: 10.3390/cancers18203296. PMID- 42938830 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 13 TI - Beyond Platinum Resistance: Reconsidering Platinum Sensitivity as a Dynamic Phenotype in Ovarian Cancer. LID - 10.3390/cancers18203295 [doi] LID - 3295 AB - Platinum-based chemotherapy remains a key treatment for epithelial ovarian cancer, but platinum resistance is a major cause of treatment failure and mortality. Traditionally, platinum resistance has been defined by the interval between the last platinum treatment and disease progression, with progression within six months generally classified as platinum-resistant. This six-month platinum-free interval has long provided a useful framework for treatment selection. However, growing preclinical and clinical evidence suggests that this interval should not be treated as a strict biological boundary. Platinum susceptibility can change as tumors evolve, with some tumors retaining residual sensitivity while others develop stable or potentially modifiable resistance mechanisms. These mechanisms include restoration of homologous recombination, altered DNA-damage tolerance, epigenetic regulation, changes in platinum transport and detoxification, adaptive survival signaling, and alterations in cell-death pathways. Some resistance mechanisms are relatively stable, whereas others may remain biologically plastic and potentially amenable to therapeutic modification. This distinction has important implications for platinum rechallenge. Responses to platinum reintroduction in selected patients previously classified as platinum-resistant indicate that the six-month interval does not fully capture the biology of platinum responsiveness. However, renewed clinical activity after rechallenge should not automatically be interpreted as biological resensitization. True resensitization requires evidence that a defined resistance mechanism has been modified and that this alteration increases platinum susceptibility. Preclinical studies have identified multiple strategies to modify platinum-resistance pathways, but clinical translation remains limited. This perspective therefore proposes that platinum resistance should be understood as a clinically defined phenotype encompassing biologically heterogeneous states, some of which may be stable and others potentially reversible. Recognizing this distinction has implications for biomarker development, treatment selection, trial design, and interpretation of platinum rechallenge. Prospective studies incorporating longitudinal tumor profiling and functional assessments of platinum susceptibility may ultimately identify patients in whom platinum activity can be therapeutically restored. FAU - Goyeneche, Alicia A AU - Goyeneche AA AD - Cancer Research Program, Research Institute, McGill University Health Centre, Montreal, QC H4A 3J1, Canada. FAU - Rosato, Adriana E AU - Rosato AE AUID- ORCID: 0000-0001-6115-6955 AD - Center for Molecular Medicine, MaineHealth Institute for Research, Scarborough, Maine, ME 04074, USA. FAU - Rosato, Roberto R AU - Rosato RR AD - Center for Molecular Medicine, MaineHealth Institute for Research, Scarborough, Maine, ME 04074, USA. FAU - Telleria, Carlos M AU - Telleria CM AUID- ORCID: 0000-0003-1070-3538 AD - Cancer Research Program, Research Institute, McGill University Health Centre, Montreal, QC H4A 3J1, Canada. AD - Department of Pathology, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC H3A 2B4, Canada. AD - Gerald Bronfman Department of Oncology, Faculty of Medicine and Health Sciences, McGill University, Montreal, QC H4A 3T2, Canada. LA - eng GR - McGill University/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702788 OTO - NOTNLM OT - Pt rechallenge OT - Pt resensitization OT - Pt sensitivity OT - biomarkers OT - carboplatin OT - cisplatin OT - ovarian cancer OT - platinum (Pt) resistance OT - tumor evolution COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/13 CRDT- 2026/10/27 10:31 PHST- 2026/09/09 00:00 [received] PHST- 2026/10/06 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - cancers18203295 [pii] AID - cancers-18-03295 [pii] AID - 10.3390/cancers18203295 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 13;18(20):3295. doi: 10.3390/cancers18203295. PMID- 42938829 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 13 TI - Clinicopathologic Characteristics, Molecular Profile, Treatment, and Recurrence in Endometrial Cancer with Pelvic Lymph Node Isolated Tumor Cells. LID - 10.3390/cancers18203293 [doi] LID - 3293 AB - OBJECTIVE: Our objective was to characterize the molecular-pathologic profile and recurrence patterns of endometrial cancer with pelvic lymph node isolated tumor cells (ITCs). METHODS: A single-center retrospective study of patients with endometrial cancer and ITCs was conducted. Patients with concurrent lymph nodes or distant metastasis were excluded. Cases were reviewed by a gynecologic pathologist, and electronic medical records were reviewed. Descriptive statistics were performed using R 4.4.2. RESULTS: A total of 43 patients were included, with a median follow-up time of 33 months. The majority were endometrioid carcinoma (91%) and stage I (72%). In total, 28 (65%) were MMR-proficient and p53-wild-type (MMRp p53wt), nine (21%) were MMR-deficient (MMRd), six (14%) were p53-abnormal (p53abn), and none were POLE-mutated (POLEmut). Additionally, 27 (96%) MMRp p53wt cases showed a microcystic, elongated, and fragmented (MELF) pattern of invasion. Adjuvant therapy was indicated in 38 (88%) patients; 32 (74%) received therapy and six (14%) declined. Four (9.3%) patients recurred, with a recurrence rate of 33% (2/6) for p53abn, 11% (1/9) for MMRd, and 4% (1/28) for MMRp p53wt. All recurrences were to distant sites. In the MMRp p53wt group, 16 (57%) had external beam radiation (EBRT) +/- vaginal brachytherapy (VBT), three (11%) had chemotherapy +/- radiation, and nine (32%) had no adjuvant treatment or VBT alone. CONCLUSIONS: Molecular profile is prognostically relevant in endometrial carcinoma with ITCs. ITCs in MMRp p53wt endometrioid carcinoma present a low risk of disease recurrence. As the majority of our cohort received adjuvant therapy, future work may explore de-escalating treatment in low-risk ITC cases. FAU - Frerichs, Haley D AU - Frerichs HD AUID- ORCID: 0009-0009-7267-7428 AD - Faculty of Medicine & Dentistry, University of Alberta, Edmonton, AB T6G 2R3, Canada. FAU - Lee, Cheng-Han AU - Lee CH AUID- ORCID: 0000-0003-2094-1222 AD - Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2R3, Canada. FAU - Wiebe, Ericka AU - Wiebe E AD - Cross Cancer Institute, Department of Oncology, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, AB T6G 2R3, Canada. FAU - Pin, Sophia AU - Pin S AUID- ORCID: 0000-0003-2264-044X AD - Cross Cancer Institute, Department of Obstetrics & Gynecology, Division of Gynecologic Oncology, Faculty of Medicine & Dentistry, University of Alberta, Edmonton, AB T6G 2R3, Canada. LA - eng GR - Women and Children's Health Research Institute/ GR - University of Alberta/ PT - Journal Article DEP - 20261013 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702782 OTO - NOTNLM OT - endometrial cancer OT - isolated tumor cells OT - sentinel lymph node biopsy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/13 CRDT- 2026/10/27 10:31 PHST- 2026/09/15 00:00 [received] PHST- 2026/10/03 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - cancers18203293 [pii] AID - cancers-18-03293 [pii] AID - 10.3390/cancers18203293 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 13;18(20):3293. doi: 10.3390/cancers18203293. PMID- 42938828 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261029 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 13 TI - Long-Term Outcomes of Cryoablation for Solitary De Novo T1a Renal Cell Carcinoma: A Tertiary Cancer Center Experience. LID - 10.3390/cancers18203292 [doi] LID - 3292 AB - Objective: To assess the long-term oncologic and survival outcomes of percutaneous cryoablation (PCA) for patients with solitary de novo T1a renal cell carcinoma (RCC). Methods: This retrospective study was conducted following the STROBE guidelines. We included patients with a single, biopsy-confirmed, treatment-naive T1a RCC (<4 cm) who underwent PCA between January 2008 and December 2021. Patient demographics, tumor characteristics, and procedural details were collected. Survival analyses were performed using the Kaplan-Meier estimator. Results: Eighty-six patients (57 men and 29 women) with a median age of 69 years met the inclusion criteria. Fifty patients (58%) had a history of an additional non-renal malignancy. In total, 86 renal tumors were treated using PCA, with a median tumor diameter of 2.7 cm. Clear cell RCC accounted for 67.4% of the tumors. Procedural complications classified as Clavien-Dindo grade III or higher occurred in 4.6% of cases. Local tumor recurrence at the ablation site was observed in five patients (5.8%), and four patients (4.6%) developed new lesions elsewhere within the kidney. The estimated median overall survival was 10.34 years. The 10-year local recurrence-free and disease-free survival rates were 93.5% and 84.0%, respectively. Both metastasis-free survival and cancer-specific survival rates at 10 years were 100%. Conclusions: Percutaneous cryoablation provided durable local tumor control with low major morbidity and favorable long-term oncologic outcomes in this selected cohort of patients with solitary cT1a renal tumors. FAU - Abdelsalam, Mohamed E AU - Abdelsalam ME AUID- ORCID: 0000-0003-1066-2641 AD - Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Unit 1471, Houston, TX 77030, USA. FAU - Awad, Ahmed AU - Awad A AD - Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Unit 1471, Houston, TX 77030, USA. FAU - Raymond, Robert J AU - Raymond RJ AD - Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Unit 1471, Houston, TX 77030, USA. FAU - Wang, Zhongya AU - Wang Z AD - Department of Biostatistics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA. FAU - Irwin, David AU - Irwin D AD - Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Unit 1471, Houston, TX 77030, USA. FAU - Habibollahi, Peiman AU - Habibollahi P AD - Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Unit 1471, Houston, TX 77030, USA. FAU - Metwalli, Zeyad A AU - Metwalli ZA AD - Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Unit 1471, Houston, TX 77030, USA. FAU - Lee, Stephen R AU - Lee SR AD - Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Unit 1471, Houston, TX 77030, USA. FAU - Odisio, Bruno C AU - Odisio BC AUID- ORCID: 0000-0002-1771-8233 AD - Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Unit 1471, Houston, TX 77030, USA. FAU - Lu, Thomas AU - Lu T AD - Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Unit 1471, Houston, TX 77030, USA. FAU - Cotta, Brittney H AU - Cotta BH AD - Department of Urology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA. FAU - Karam, Jose A AU - Karam JA AD - Department of Urology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA. FAU - Matin, Surena F AU - Matin SF AUID- ORCID: 0000-0003-1638-1445 AD - Department of Urology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA. FAU - Ahrar, Kamran AU - Ahrar K AUID- ORCID: 0000-0002-7800-2300 AD - Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Boulevard, Unit 1471, Houston, TX 77030, USA. LA - eng GR - P30 CA016672/CA/NCI NIH HHS/United States PT - Journal Article DEP - 20261013 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702555 OTO - NOTNLM OT - ablation OT - outcomes OT - renal cell carcinoma OT - survival rates COIS- The author declares no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/13 CRDT- 2026/10/27 10:31 PHST- 2026/09/23 00:00 [received] PHST- 2026/10/10 00:00 [revised] PHST- 2026/10/12 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/13 00:00 [pmc-release] AID - cancers18203292 [pii] AID - cancers-18-03292 [pii] AID - 10.3390/cancers18203292 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 13;18(20):3292. doi: 10.3390/cancers18203292. PMID- 42938826 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 12 TI - Organoid-Guided Synthetic Micropeptide Therapeutics to Target Ovarian Cancer Stem Cells. LID - 10.3390/cancers18203289 [doi] LID - 3289 AB - Ovarian cancer remains the most lethal gynecological malignancy, largely due to high recurrence rates and the development of therapy-resistant disease. Cancer stem cells (CSCs) contribute to treatment failure by promoting chemoresistance through signaling pathways such as Wnt/beta-catenin, Hedgehog, and Notch, as well as through dynamic interactions with the tumor microenvironment (TME). Ovarian CSC (OCSC)-associated biomarkers, including CD44, CD24, CD133, and ALDH, have expanded opportunities for specific tumor characterization and biomarker identification. Effective targeting of OCSCs requires experimental models that preserve cellular plasticity and hierarchical organization. Development of synthetic matrices and chemically defined media has improved the reproducibility and scalability of three-dimensional (3D) organoid cultures for preclinical drug testing. CSC-derived organoids, established by enriching cells positive for CSC markers directly from patient tumor material, provide a 3D platform for studying OCSC-associated phenotypes and therapeutic responses. This review examines current understanding of ovarian CSC biology and the development of OCSC-derived 3D organoid models that can recapitulate cellular architecture and patient-specific drug responses. These 3D organoid models provide a guided platform for evaluating emerging therapeutics, such as micropeptides encoded by short open reading frames or engineered from functional tumor-suppressor protein domains selected to target OCSCs. Their small size enables modulation of intracellular signaling and highly specific protein-protein interactions. We further review micropeptide stabilization strategies and tumor-targeted delivery systems designed to enhance bioavailability. This review highlights how integrating patient-specific OCSC-derived organoid models with engineered micropeptides may accelerate the development of personalized treatment strategies for ovarian cancer while improving the preclinical evaluation of novel therapies. FAU - Balayoganandh, Bhavana AU - Balayoganandh B AUID- ORCID: 0009-0005-8632-6582 AD - Department of Biotechnology, Faculty of Biomedical Sciences and Technology, Sri Ramachandra Institute of Higher Education and Research, Chennai 600116, Tamil Nadu, India. FAU - Gamit, Naisarg AU - Gamit N AUID- ORCID: 0000-0002-2871-9565 AD - Division of Cancer Stem Cells and Cardiovascular Regeneration, Manipal Institute of Regenerative Medicine, Manipal Academy of Higher Education (MAHE), Bangalore 560063, Karnataka, India. FAU - Kallepalli, Harshit AU - Kallepalli H AUID- ORCID: 0009-0005-4180-7024 AD - Department of Biotechnology, Faculty of Biomedical Sciences and Technology, Sri Ramachandra Institute of Higher Education and Research, Chennai 600116, Tamil Nadu, India. FAU - Dev, Bhawna AU - Dev B AUID- ORCID: 0000-0003-1771-9743 AD - Department of Radiology, Sri Ramachandra Institute of Higher Education and Research, Chennai 600116, Tamil Nadu, India. FAU - Dharmarajan, Arun AU - Dharmarajan A AUID- ORCID: 0000-0002-6715-6005 AD - Trichy SRM Medical College Hospital and Research Center, Trichy 621105, Tamil Nadu, India. AD - School of Human Sciences, Faculty of Life and Physical Sciences, The University of Western Australia, Perth, WA 6009, Australia. AD - Faculty of Health Sciences, Curtin Medical School, Curtin University, Perth, WA 6102, Australia. FAU - Warrier, Sudha AU - Warrier S AUID- ORCID: 0000-0003-3787-5647 AD - Faculty of Life Sciences, Atria University, Bangalore 560024, Karnataka, India. LA - eng GR - BT/PR41903/MED/97/527/ 2021/Department of Biotechnology/ PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702386 OTO - NOTNLM OT - 3D disease modelling OT - chemoresistance OT - nanomedicine OT - ovarian cancer OT - ovarian cancer stem cells OT - patient-derived organoids OT - peptide engineering OT - precision oncology OT - reproductive health OT - synthetic micropeptides OT - targeted drug delivery COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/12 CRDT- 2026/10/27 10:31 PHST- 2026/08/10 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/07 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - cancers18203289 [pii] AID - cancers-18-03289 [pii] AID - 10.3390/cancers18203289 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 12;18(20):3289. doi: 10.3390/cancers18203289. PMID- 42938825 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 12 TI - Defined Extracellular Matrix Contexts Rewire 3D Breast Cancer Cell Models: Effects of Sulfated Hyaluronan in the Expression of Matrix Effector and Matrix-Based Cell Functional Properties. LID - 10.3390/cancers18203288 [doi] LID - 3288 AB - Background: Breast cancer progression is strongly influenced by tumor cell interactions with the extracellular matrix (ECM). Three-dimensional (3D) cell culture models provide more relevant systems for studying aspects of the tumor microenvironment (TME), including tumor architecture, matrix remodeling and cell-ECM communication. Collagen type I (Col I), fibronectin (FN) and hyaluronan (HA) are key ECM components involved in breast cancer progression. At the same time, integrins act as major matrix-sensing receptors that translate ECM cues into cellular responses. In this study, we investigated how defined ECM contexts regulate functional cell properties in shERbeta MDA-MB-231 and Hs578T advanced 3D breast cancer cell models. Methods: Cells were cultured in 2D and 3D systems under matrix-free conditions or in Col I, FN, and mixed Col I/FN environments, in the presence or absence of HA or sulfated HA (sHA). Spheroid growth, matrix-dependent spheroid organization and dissemination were evaluated. In parallel, selected collagen- and fibronectin-associated integrin subunits, together with HA-related molecules including HAS2, CD44, and RHAMM, were analyzed to assess receptor-mediated matrix- and HA-associated responses. Results: Matrix substrates strongly influenced breast cancer spheroid behavior. Col I promoted outward dissemination, whereas FN-containing environments favored compact organization and restricted spreading. HA supported spheroid-like organization in a matrix-dependent manner, whereas sHA disrupted organized spheroid assembly in ECM-rich conditions, resulting in looser structures or smaller cell colonies, depending on the matrix context. Gene expression analysis revealed model-dependent remodeling of matrix- and HA-associated programs, with broader adhesion-related responses in shERbeta MDA-MB-231 cells and stronger dimensionality-driven regulation in Hs578T cells. Conclusions: Overall, these findings demonstrate that defined ECM contexts actively regulate breast cancer spheroid behavior and matrix-associated receptor programs. Col I, FN and mixed Col I/FN environments generated distinct 3D phenotypes, affecting spheroid growth, organization and dissemination, while HA/sHA further modified these matrix-dependent responses. This study supports sHA as a functional matrix molecule with anti-cancer relevance and highlights the value of 3D matrix-based models for investigating tumor-matrix communication in breast cancer. FAU - Vetoulas, Marios AU - Vetoulas M AUID- ORCID: 0009-0004-5964-4692 AD - Biochemistry, Biochemical Analysis & Matrix Pathobiology Research Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26504 Patras, Greece. FAU - Drosinou, Maria AU - Drosinou M AD - Biochemistry, Biochemical Analysis & Matrix Pathobiology Research Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26504 Patras, Greece. FAU - Alexiou, Nikoleta AU - Alexiou N AUID- ORCID: 0009-0007-3499-4022 AD - Biochemistry, Biochemical Analysis & Matrix Pathobiology Research Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26504 Patras, Greece. FAU - Farmaki, Angeliki AU - Farmaki A AD - Biochemistry, Biochemical Analysis & Matrix Pathobiology Research Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26504 Patras, Greece. FAU - Piperigkou, Zoi AU - Piperigkou Z AUID- ORCID: 0000-0002-0472-5389 AD - Biochemistry, Biochemical Analysis & Matrix Pathobiology Research Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26504 Patras, Greece. FAU - Karamanos, Nikos K AU - Karamanos NK AUID- ORCID: 0000-0003-3618-0288 AD - Biochemistry, Biochemical Analysis & Matrix Pathobiology Research Group, Laboratory of Biochemistry, Department of Chemistry, University of Patras, 26504 Patras, Greece. LA - eng GR - Grant agreement ID: 101130985 "CARES-Human ECM-based platform for anti-cancer drug testing/European Union/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702652 OTO - NOTNLM OT - 3D spheroids OT - CD44 OT - HAS2 OT - RHAMM OT - breast cancer OT - collagen type I OT - extracellular matrix OT - fibronectin OT - hyaluronan OT - integrins OT - sulfated hyaluronan OT - triple-negative breast cancer OT - tumor microenvironment COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/12 CRDT- 2026/10/27 10:31 PHST- 2026/09/26 00:00 [received] PHST- 2026/10/08 00:00 [revised] PHST- 2026/10/08 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - cancers18203288 [pii] AID - cancers-18-03288 [pii] AID - 10.3390/cancers18203288 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 12;18(20):3288. doi: 10.3390/cancers18203288. PMID- 42938822 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 12 TI - Photodynamic Bone Stabilization in Pathologic Humeral Fractures: Implant Safety, Pain Relief and Functional Outcomes. LID - 10.3390/cancers18203285 [doi] LID - 3285 AB - Background: Pathologic fractures of the humerus due to metastatic disease cause severe pain, loss of function and reduced quality of life. Surgical treatment is predominantly palliative and aims to achieve rapid pain relief, early functional recovery and minimal treatment-related morbidity. The photodynamic bone stabilization system (IlluminOss((R))) is a minimally invasive, radiolucent, intramedullary device that may address these needs. Methods: This retrospective analysis of prospectively collected data from two centers included 51 patients with 52 pathologic humeral lesions (completed or impending fractures) treated with photodynamic intramedullary stabilization between 2013 and 2025. Baseline variables comprised demographics, Eastern Cooperative Oncology Group (ECOG) performance status, comorbidities, pain (numeric rating scale, NRS) and function (Musculoskeletal Tumor Society Upper Extremity Score, MSTS-UE; Toronto Extremity Salvage Score, TESS). Lesion characteristics, operative details, in-hospital course, follow-up outcomes (TESS, patient satisfaction/quality of life) and long-term endpoints (survival, complications) were recorded. Results: The study cohort (25 women, 26 men; mean age 68.5 years) represented a highly palliative population with advanced, disseminated malignant disease. Of the 52 humeral lesions, 35 (67.3%) presented as pathological fractures, whereas 17 lesions (32.7%) were classified as impending fractures due to extensive osteolysis. Among the impending fractures, Mirels scores ranged from 8 to 10, with three lesions scoring 8, eight lesions scoring 9, and six lesions scoring 10. ECOG performance status was 0-2 in 75.0% and 3-4 in 25.0% of patients. Preoperative pain was high (mean NRS 7.1) and function markedly reduced (median MSTS-UE 9.5/30; median TESS 36.5/100). Postoperatively, pain improved significantly from NRS 7.1 to 2.0 (p < 0.001). MSTS-UE increased from a median of 9.5 to 20 (p < 0.001). Follow-up showed further functional improvement with an increased TESS of 36.5 to 61 (p < 0.001), high patient satisfaction and subjective improvement in quality of life. Kaplan-Meier analysis (n = 44) demonstrated a median survival of 6 months for ECOG 0-2 and 3 months for ECOG 3-4. Implant failure (Henderson type III) occurred four times (7.7%) and was successfully revised with plate and/or screw osteosynthesis or diaphyseal replacement. Conclusions: Photodynamic intramedullary stabilization provides effective treatment for selected pathologic humeral lesions with low implant failure rate, offering rapid pain relief, functional improvement and low perioperative morbidity. Implant failure in complex fracture morphologies highlights the need for morphology-adapted indication and consideration of supplemental fixation in high-risk cases. FAU - Haupt, Julian AU - Haupt J AD - Department for Trauma Surgery and Orthopaedics, Reconstructive and Septic Surgery, and Sports Traumatology, German Armed Forces Hospital Ulm, 89081 Ulm, Germany. FAU - Jung, Beatrice AU - Jung B AUID- ORCID: 0009-0003-8319-6773 AD - Department of Orthopedics and Traumatology, University Medical Center Mainz, 55131 Mainz, Germany. FAU - Farah, George AU - Farah G AD - Department of Orthopedics and Traumatology, University Medical Center Mainz, 55131 Mainz, Germany. FAU - Drees, Philipp AU - Drees P AD - Department of Orthopedics and Traumatology, University Medical Center Mainz, 55131 Mainz, Germany. FAU - Gercek, Erol AU - Gercek E AD - Department of Orthopedics and Traumatology, University Medical Center Mainz, 55131 Mainz, Germany. FAU - Sachsenmaier, Saskia AU - Sachsenmaier S AD - Department of Orthopedic Surgery, University Hospital of Tubingen, 72076 Tubingen, Germany. FAU - Ring, Matthias AU - Ring M AD - Department for Trauma Surgery and Orthopaedics, Reconstructive and Septic Surgery, and Sports Traumatology, German Armed Forces Hospital Ulm, 89081 Ulm, Germany. FAU - Traub, Frank AU - Traub F AUID- ORCID: 0000-0002-6400-3257 AD - Department of Orthopedics and Traumatology, University Medical Center Mainz, 55131 Mainz, Germany. LA - eng PT - Journal Article DEP - 20261012 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702513 OTO - NOTNLM OT - bone metastases OT - multiple myeloma OT - palliative orthopedic oncology OT - pathologic humeral fracture OT - photodynamic intramedullary bone stabilization OT - skeletal-related events COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/12 CRDT- 2026/10/27 10:31 PHST- 2026/08/06 00:00 [received] PHST- 2026/09/28 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - cancers18203285 [pii] AID - cancers-18-03285 [pii] AID - 10.3390/cancers18203285 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 12;18(20):3285. doi: 10.3390/cancers18203285. PMID- 42938821 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 12 TI - Complications of Percutaneous Thermal Ablation of Primary and Metastatic Lung Tumors: Review of Prevention and Management Strategies. LID - 10.3390/cancers18203284 [doi] LID - 3284 AB - Pulmonary malignancies encompassing both primary lung cancer and metastatic lesions are a leading cause of cancer-related mortality globally. Percutaneous thermal ablation of primary and metastatic lung tumors using radiofrequency ablation, microwave ablation, or cryoablation has emerged as an established minimally invasive treatment modality for selected patients unsuitable for surgery or requiring lung preservation. Although most lung ablation complications are self-limiting, some adverse events may result in long-term morbidity or mortality. Post-ablation pneumothorax has the highest reported incidence, with pooled incidences of 37-44%, although rates may vary according to patient cohorts and study definitions. As indications for percutaneous thermal ablation of pulmonary lesions expand to include complex lesions or higher-risk patients, the prevention and management of procedure-related complications remain central to safe clinical practice. Although prior reviews have described the techniques, oncological outcomes, and complication profiles of image-guided lung ablation, less emphasis has been placed on integrating practical complication prevention and management strategies throughout the periprocedural workflow. This narrative review aims to synthesize various complication prevention and management strategies for percutaneous thermal ablation of both primary and metastatic pulmonary lesions. The review focuses on both common and rare complications, and various aspects of complication prevention such as patient/lesion selection, procedural planning, and anesthesia/respiratory control. A comprehensive understanding of complication profiles and mitigation strategies is essential to optimize procedural safety, guide patient selection, and establish ablative techniques as a pillar in multidisciplinary thoracic oncology care. FAU - How, Guo Yuan AU - How GY AUID- ORCID: 0000-0002-2104-899X AD - Department of Diagnostic Radiology, Tan Tock Seng Hospital, Singapore 308433, Singapore. FAU - Pua, Uei AU - Pua U AD - Department of Diagnostic Radiology, Tan Tock Seng Hospital, Singapore 308433, Singapore. LA - eng PT - Journal Article PT - Review DEP - 20261012 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702536 OTO - NOTNLM OT - ablation OT - complications OT - lung cancer OT - prevention COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/12 CRDT- 2026/10/27 10:31 PHST- 2026/09/17 00:00 [received] PHST- 2026/09/29 00:00 [revised] PHST- 2026/10/01 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - cancers18203284 [pii] AID - cancers-18-03284 [pii] AID - 10.3390/cancers18203284 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 12;18(20):3284. doi: 10.3390/cancers18203284. PMID- 42938819 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 12 TI - Development and Internal Validation of the BRICS Score: A Preoperative Clinical and Hematological Model for Predicting High-Grade and Muscle-Invasive Bladder Cancer. LID - 10.3390/cancers18203282 [doi] LID - 3282 AB - BACKGROUND/OBJECTIVES: We developed and internally validated the Bladder Risk Index using Clinical and Systemic biomarkers (BRICS), a preoperative prediction model designed to estimate the likelihood of high-grade (HG) disease and muscle-invasive bladder cancer (MIBC) in patients undergoing their first transurethral resection of bladder tumor (TURBT). METHODS: This retrospective single-center study included 161 patients with newly diagnosed urothelial bladder cancer. The BRICS-HG model included tumor size, neutrophil-to-lymphocyte ratio (NLR), and creatinine-to-hemoglobin ratio (CHR), while the BRICS-MIBC model was based on tumor size and NLR. Model performance was assessed in terms of discrimination, calibration, and Brier score, together with exploratory decision curve analysis. Internal validation was performed using 2000 bootstrap resamples. RESULTS: MIBC was identified in 20 patients (12.4%). BRICS-HG demonstrated limited discriminatory ability, with an apparent AUC of 0.649 and an optimism-corrected AUC of 0.623 (95% bootstrap interval: 0.541-0.703). BRICS-MIBC achieved moderate discrimination, with an apparent AUC of 0.735 and an optimism-corrected AUC of 0.709 (95% bootstrap interval: 0.588-0.826). Across the bootstrap resamples, the model combining tumor size and NLR was selected in 53.9% of cases, reflecting uncertainty in predictor selection. CONCLUSIONS: BRICS-HG should be regarded as an exploratory model, and its current performance does not support clinical application. BRICS-MIBC demonstrated moderate discrimination after internal validation; however, its performance should be interpreted cautiously given the small number of MIBC events. Validation in larger, independent cohorts, ideally across multiple centers, is necessary before the model can be considered for clinical implementation. FAU - Gherasim, Raul-Dumitru AU - Gherasim RD AUID- ORCID: 0009-0002-4778-380X AD - Department of Urology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540139 Targu Mures, Romania. AD - Department of Urology, Targu Mures Clinical Hospital, P-ta Bernady Gyorgy, Nr. 6, 540072 Targu-Mures, Romania. FAU - Porav-Hodade, Daniel AU - Porav-Hodade D AUID- ORCID: 0009-0003-4279-673X AD - Department of Urology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540139 Targu Mures, Romania. AD - Department of Urology, Targu Mures Clinical Hospital, P-ta Bernady Gyorgy, Nr. 6, 540072 Targu-Mures, Romania. FAU - Chibelean, Calin AU - Chibelean C AD - Department of Urology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540139 Targu Mures, Romania. AD - Department of Urology, Targu Mures Clinical Hospital, P-ta Bernady Gyorgy, Nr. 6, 540072 Targu-Mures, Romania. FAU - Voidazan, Septimiu AU - Voidazan S AD - Department of Epidemiology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540139 Targu Mures, Romania. FAU - Todea-Moga, Ciprian AU - Todea-Moga C AD - Department of Urology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540139 Targu Mures, Romania. AD - Department of Urology, Targu Mures Clinical Hospital, P-ta Bernady Gyorgy, Nr. 6, 540072 Targu-Mures, Romania. FAU - Vida, Oliver-Arpad AU - Vida OA AD - Department of Urology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540139 Targu Mures, Romania. AD - Department of Urology, Targu Mures Clinical Hospital, P-ta Bernady Gyorgy, Nr. 6, 540072 Targu-Mures, Romania. FAU - Ghirca, Veronica-Maria AU - Ghirca VM AD - Department of Urology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540139 Targu Mures, Romania. AD - Department of Urology, Targu Mures Clinical Hospital, P-ta Bernady Gyorgy, Nr. 6, 540072 Targu-Mures, Romania. FAU - Vascul, Rares-Florin AU - Vascul RF AD - Department of Urology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540139 Targu Mures, Romania. AD - Department of Urology, Targu Mures Clinical Hospital, P-ta Bernady Gyorgy, Nr. 6, 540072 Targu-Mures, Romania. FAU - Reman, Tibor Lorand AU - Reman TL AUID- ORCID: 0009-0005-8009-3482 AD - Department of Urology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540139 Targu Mures, Romania. AD - Department of Urology, Targu Mures Clinical Hospital, P-ta Bernady Gyorgy, Nr. 6, 540072 Targu-Mures, Romania. FAU - Martha, Orsolya Katalin Ilona AU - Martha OKI AD - Department of Urology, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Targu Mures, 540139 Targu Mures, Romania. AD - Department of Urology, Targu Mures Clinical Hospital, P-ta Bernady Gyorgy, Nr. 6, 540072 Targu-Mures, Romania. LA - eng PT - Journal Article DEP - 20261012 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702414 OTO - NOTNLM OT - BRICS OT - NLR OT - bladder cancer OT - high-grade bladder cancer OT - muscle-invasive bladder cancer OT - prediction model OT - risk stratification OT - systemic inflammation COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/12 CRDT- 2026/10/27 10:31 PHST- 2026/09/01 00:00 [received] PHST- 2026/09/30 00:00 [revised] PHST- 2026/10/04 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - cancers18203282 [pii] AID - cancers-18-03282 [pii] AID - 10.3390/cancers18203282 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 12;18(20):3282. doi: 10.3390/cancers18203282. PMID- 42938816 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 12 TI - Comparative Neoplasm Risk of Mirabegron and Antimuscarinic Therapy in Overactive Bladder: A Multicenter Target Trial Emulation. LID - 10.3390/cancers18203279 [doi] LID - 3279 AB - Background/Aims: Mirabegron, a selective beta3-adrenergic receptor agonist used to treat overactive bladder (OAB), can promote adipose tissue browning and alter systemic metabolism, raising interest in its potential relationship with cancer development. However, clinical evidence regarding cancer risk remains limited and inconsistent. We compared the risks of obesity-related and site-specific cancers between patients with OAB initiating mirabegron and those initiating antimuscarinic medications. Methods: We conducted a multicenter emulated target trial using the TriNetX Global Collaborative Network. Adults aged >/=20 years with OAB or related symptoms who newly initiated mirabegron or an antimuscarinic medication were included using a new-user, active-comparator design. Patients with pre-existing malignancy were excluded. Propensity score matching was performed 1:1, and a 1-year lag period after treatment initiation was applied for cancer outcomes to reduce potential reverse causation from occult malignancy. Cox proportional hazards models estimated hazard ratios (HRs) and 95% confidence intervals (CIs) under an intention-to-treat analog. Results: A 365-day lag/landmark period was applied before propensity-score matching. Patients with a recorded neoplasm or death during this period, or without follow-up extending through day 366, were excluded. Subsequent 1:1 propensity-score matching yielded 106,500 patients in each treatment group. Mirabegron initiation was not associated with the composite of obesity-related neoplasms compared with antimuscarinic initiation (HR, 1.011; 95% CI, 0.954-1.071). The individual neoplasm outcomes also showed no significant between-group differences. However, among patients eligible for the 1-year landmark mortality analysis, mirabegron initiation was associated with higher subsequent all-cause mortality (HR, 1.083; 95% CI, 1.044-1.124). Exploratory negative-control analysis of Hodgkin lymphoma showed no significant association. Conclusions: Among patients with OAB, mirabegron initiation was not associated with a significant difference in overall obesity-related neoplasm risk compared with antimuscarinic initiation. The observed associations with all-cause mortality should be interpreted cautiously given the observational design, multiple comparisons, and potential residual confounding. These findings are hypothesis-generating and require confirmation in prospective studies. FAU - Yen, Fu-Shun AU - Yen FS AUID- ORCID: 0000-0002-4677-2415 AD - Dr. Yen's Clinic, No. 15, Shanying Road, Gueishan District, Taoyuan 33354, Taiwan. FAU - Wei, James Cheng-Chung AU - Wei JC AD - Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan. AD - Department of Allergy, Immunology & Rheumatology, Chung Shan Medical University Hospital, No. 110, Sec. 1, Jianguo N. Rd., South District, Taichung 40201, Taiwan. AD - Graduate Institute of Integrated Medicine, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan. FAU - Hsu, Chih-Cheng AU - Hsu CC AUID- ORCID: 0000-0003-4563-4341 AD - Institute of Population Health Sciences, National Health Research Institutes, 35 Keyan Road, Zhunan, Miaoli County 35053, Taiwan. AD - Department of Health Services Administration, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan. AD - Department of Family Medicine, Min-Sheng General Hospital, 168 ChingKuo Road, Taoyuan 33044, Taiwan. AD - National Center for Geriatrics and Welfare Research, National Health Research Institutes, No. 95-1, Xuefu Rd., Huwei Township, Yunlin County 632007, Taiwan. FAU - Hwu, Chii-Min AU - Hwu CM AUID- ORCID: 0000-0002-8209-9627 AD - Section of Endocrinology and Metabolism, Department of Medicine, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Road, Beitou District, Taipei 11217, Taiwan. AD - Faculty of Medicine, National Yang Ming Chiao Tung University School of Medicine, Taipei 11221, Taiwan. FAU - Hung, Yao-Min AU - Hung YM AD - Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung 81346, Taiwan. AD - Department of Internal Medicine, Taitung Hospital, Ministry of Health and Welfare, Taitung 95043, Taiwan. AD - Master Program in Biomedicine, College of Science and Engineering, National Taitung University, Taitung 95092, Taiwan. FAU - Chang, Renin AU - Chang R AD - Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung 81346, Taiwan. AD - Department of Emergency Medicine, Kaohsiung Veterans General Hospital, Kaohsiung 81346, Taiwan. AD - Department of Recreation and Sports Management, Tajen University, Pintung 90741, Taiwan. FAU - Lin, Tzu-Ping AU - Lin TP AUID- ORCID: 0000-0002-4514-6332 AD - Department of Urology, College of Medicine and Shu-Tien Urological Research Center, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan. AD - Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung 81346, Taiwan. LA - eng GR - NSTC112-2314-B-075B-020/National Science and Technology Council/ GR - KSVGH-114-071/Kaohsiung Veterans General Hospital/ GR - NSTC115-2314-B-075B-003-MY3-1/National Science and Technology Council/ GR - KSVGH115-071/Kaohsiung Veterans General Hospital/ GR - VGHUST115-G3-1-1/Kaohsiung Veterans General Hospital/ PT - Journal Article DEP - 20261012 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702379 OTO - NOTNLM OT - all-cause mortality OT - antimuscarinic medications OT - mirabegron OT - obesity-related neoplasms OT - overactive bladder OT - site-specific neoplasms COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/12 CRDT- 2026/10/27 10:31 PHST- 2026/09/02 00:00 [received] PHST- 2026/10/07 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/12 00:00 [pmc-release] AID - cancers18203279 [pii] AID - cancers-18-03279 [pii] AID - 10.3390/cancers18203279 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 12;18(20):3279. doi: 10.3390/cancers18203279. PMID- 42938814 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 11 TI - Dermoscopy at an Inflection Point: Sharper Criteria, New Optics, and the Arrival of Artificial Intelligence. LID - 10.3390/cancers18203277 [doi] LID - 3277 AB - Dermoscopy improves the diagnosis of melanoma and keratinocyte carcinoma, but its value depends on the observer, the lesion mix, the anatomic site, and the action taken after the examination. Recent reviews have mainly focused on one modality or image-classification accuracy. This narrative review follows the clinical pathway and compares five linked areas: defined diagnostic criteria, sequential digital monitoring, site- and skin-tone-specific interpretation, multimodal imaging, and artificial intelligence (AI). Melanoma and keratinocyte carcinoma are discussed separately because their criteria, diagnostic problems, imaging needs, and management differ. Meta-analyses support dermoscopy over naked-eye examination, although accuracy remains closely related to clinician experience. A randomized trial of three-dimensional total-body photography with sequential dermoscopy led to more procedures and higher costs without finding more malignant tumors over two years. Reflectance confocal microscopy can improve specificity in selected equivocal lesions, while optical coherence technologies add architectural and depth information that is most relevant to keratinocyte carcinoma. AI has moved from retrospective benchmarks to prospective multicenter studies, but false-positive burden, dataset shift, underrepresentation of darker skin tones, limited explainability, and uncertain workflow effects remain. The review also considers foundation models, regulation, and patient-facing tools and uses a SWOT analysis to define research priorities. The field is at an inflection point in evidence, not image quality. Progress should be judged by effects on biopsy decisions, missed-cancer rates, timeliness, cost, access, and patient outcomes in defined populations. FAU - Pham, Dana N AU - Pham DN AUID- ORCID: 0009-0005-6655-2144 AD - Orlando College of Osteopathic Medicine, Winter Garden, FL 34787, USA. FAU - Chan, Warren H AU - Chan WH AD - Reveal Research Institute, Dallas, TX 75235, USA. FAU - Conway, Jade AU - Conway J AUID- ORCID: 0000-0002-3193-5197 AD - Reveal Research Institute, Dallas, TX 75235, USA. FAU - Ali, Iman AU - Ali I AD - Texas Health Dallas, Dallas, TX 75231, USA. FAU - Cockerell, Clay J AU - Cockerell CJ AD - The Departments of Dermatology and Pathology, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. AD - Lake Granbury Medical Center, Dallas, TX 75235, USA. LA - eng PT - Journal Article PT - Review DEP - 20261011 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702169 OTO - NOTNLM OT - artificial intelligence OT - basal cell carcinoma OT - dermoscopy OT - melanoma OT - optical coherence tomography OT - reflectance confocal microscopy OT - squamous cell carcinoma OT - teledermoscopy COIS- The authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/11 CRDT- 2026/10/27 10:31 PHST- 2026/08/18 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - cancers18203277 [pii] AID - cancers-18-03277 [pii] AID - 10.3390/cancers18203277 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 11;18(20):3277. doi: 10.3390/cancers18203277. PMID- 42938813 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20261027 LR - 20261028 IS - 2072-6694 (Print) IS - 2072-6694 (Electronic) IS - 2072-6694 (Linking) VI - 18 IP - 20 DP - 2026 Oct 11 TI - Adjuvant Therapy in Resected Adrenocortical Carcinoma: Current Evidence and Patient Selection. LID - 10.3390/cancers18203276 [doi] LID - 3276 AB - Adrenocortical carcinoma (ACC) is a rare and aggressive malignancy with recurrence rates of 54-70% following curative-intent resection, even after microscopically complete (R0) surgery. In this review, we summarize the current evidence for adjuvant mitotane, platinum-based chemotherapy, and radiation therapy in resected ACC, and discuss patient selection based on prognostic risk stratification. We also highlight the importance of multidisciplinary management at expert centers, remaining evidence gaps, and future directions including biomarker-driven treatment strategies. FAU - Sanchez, Lina Pedraza AU - Sanchez LP AD - Department of Head and Neck-Endocrine Oncology, Moffitt Cancer Center, Tampa, FL 33612, USA. FAU - Rabinowits, Guilherme AU - Rabinowits G AD - Department of Head and Neck-Endocrine Oncology, Moffitt Cancer Center, Tampa, FL 33612, USA. FAU - Monsour, Elio AU - Monsour E AD - Department of Head and Neck-Endocrine Oncology, Moffitt Cancer Center, Tampa, FL 33612, USA. FAU - Dogra, Prerna AU - Dogra P AD - Department of Head and Neck-Endocrine Oncology, Moffitt Cancer Center, Tampa, FL 33612, USA. LA - eng PT - Journal Article PT - Review DEP - 20261011 PL - Switzerland TA - Cancers (Basel) JT - Cancers JID - 101526829 PMC - PMC13702568 OTO - NOTNLM OT - ADIUVO OT - ENSAT stage OT - adrenal cancer OT - adrenal malignancy OT - mitotane COIS- P.D. reports serving on the advisory board for Esteve Pharmaceuticals. R.G. reports serving on scientific advisory boards or as a consultant for Merus, Coherus, Fennec, Fulgent, Bicara, Genmab, Merck, TILT and Janssen. The other authors declare no conflicts of interest. EDAT- 2026/10/27 11:31 MHDA- 2026/10/27 11:32 PMCR- 2026/10/11 CRDT- 2026/10/27 10:31 PHST- 2026/08/22 00:00 [received] PHST- 2026/10/09 00:00 [revised] PHST- 2026/10/09 00:00 [accepted] PHST- 2026/10/27 11:32 [medline] PHST- 2026/10/27 11:31 [pubmed] PHST- 2026/10/27 10:31 [entrez] PHST- 2026/10/11 00:00 [pmc-release] AID - cancers18203276 [pii] AID - cancers-18-03276 [pii] AID - 10.3390/cancers18203276 [doi] PST - epublish SO - Cancers (Basel). 2026 Oct 11;18(20):3276. doi: 10.3390/cancers18203276.